LOCUS       DAOPHV010000001        18452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_3319_length_18452_cov_4.546393, whole genome shotgun sequence.
ACCESSION   DAOPHV010000001 DAOPHV010000000
VERSION     DAOPHV010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 18452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18452
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3319_length_18452_cov_4.546393"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..1071)
                     /locus_tag="PLQ29_00005"
     CDS             complement(88..1071)
                     /locus_tag="PLQ29_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85053.1"
                     /translation="MKGLRRFLGFALYLCMAFPLMLGGLAVASLAPAAGDSAALKTLV
                     VDPRFSELLRSPDLVTMAPETMRLGGSVLNGKAATAAFQASVPTSAVVDTVSGAIDAA
                     YEAFGRGDGFFYFDARPLKAAVSTGAPTFASVYVAKAALPETVTRAPGDPGRAETPIT
                     LPPGDAGKAAVEAVLLGALAAQPDQALIGEAGSRMEIPSVSWSLAASQASLWLLLSGA
                     GLCFASVMVSDGDWRRRLGTLGSRVIAPSAIILSIGLLPRLILPGQALRLSPEIAVAE
                     LPALLDYLRFVASRLGGGFLPAGLIGLGVGTALVSVKRALPLADDDEEDLE"
     gene            complement(1068..2669)
                     /locus_tag="PLQ29_00010"
     CDS             complement(1068..2669)
                     /locus_tag="PLQ29_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HPG85054.1"
                     /translation="MMKLYRLKSAWLCMAALSVCAPALVFSDGAARELVVGMGTYDVE
                     LNPYKSIYAHEMQLFTALYEGLFSYDPQSLDPVRAQAESFEKSADGKTWTFRMRAGAR
                     WADGTPVTAGDFVESWLYLLSPRTKAEYAVFFDIIQGAKDYRTGKSSSPESVGLRAPD
                     DRTLVVELTSPAAYFTRLLCHSAFVPVHRSLRGTRTWEAAAVVGNGPYVIEAMDEGSM
                     LLRRNPTYWDAGNVAVQTIKVLFLDSEKDATRRYDDGEIHWLTDMADLDALVSTDAIQ
                     FAPMFATGYYFWNSSRRPWNDARLRRALALLVPWDRIRTEDNYYAPSSSLVLPFAGYE
                     SPAGIDRRDEEEAMRLLAQAGHPGGEGLPALRFVYYESETHEANLAVIEEAWNAIGVR
                     VERARVPAGESIRDHRQAGFDLSFTSWIGDFADPAAFLLMWTADSGLNEGGYRSREYD
                     ALMARSMEEEGKARLATLAQAEAKLLSDAPLLPLYHSVSFNVIDLDAVTGWFQNPLDI
                     HPFKSMGFGAPKARPYVALAGGTAR"
     gene            complement(2700..4118)
                     /locus_tag="PLQ29_00015"
     CDS             complement(2700..4118)
                     /locus_tag="PLQ29_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85055.1"
                     /translation="MEDSVVYADRLERALSAKIEHMDGSELKQLKDDFKLFQSAFQAI
                     YNVLLKKGLISEDPYKYDLKISEVTTPSESPFAESEKIDQISIRLSQYESYLDFLNNY
                     YQFSVEFLTMGRIKRLASLTKYFAFTQFTENSQHINTRYFAEIVGLVKKGTDPLSTGI
                     INEGLLQLDKASRKIFQTLKALTLIHKERYKLELRRLVMNGLALDRDYAVTHQDDAVR
                     KIRQKFTEVAGDRPFYAELVAEALKEDYGAEGESLRDGILAALKVAEAKSGDKSAERN
                     YKAALLDGARVLIGVGFQLEDAVRKLEENQALLESMDRSFLTKVKRAIREMLGKKGEH
                     VVHEVEYLDPVTSERKNESIDFTAFCREAAKRAQSLAAMVSKSSAAFKRMEGSSEDVI
                     YKFLSKSIEELQAFHRKLSALDEFFQGAIEDPEFKGRVRSVKIELGAIKNAIIKSNQK
                     RHEYIAQKEELEQMKRLGIREA"
     gene            4225..>4579
                     /locus_tag="PLQ29_00020"
     CDS             4225..>4579
                     /locus_tag="PLQ29_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85056.1"
                     /translation="MEPTLSYRLFAARFAPARLFAVAAAAMAAFVACQAASEMEAAIR
                     LPTMPSAWAGFGGAPGAGLWELSWLSASGSGGPFPAAPGAVVALRLPRGEEAAVFCRA
                     VLGAERSLPYGAAWPQ"
     assembly_gap    4580..4589
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4914..6764)
                     /locus_tag="PLQ29_00025"
     CDS             complement(4914..6764)
                     /locus_tag="PLQ29_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG85057.1"
                     /translation="MRRARPGTRTFTALFLLSAAAVAIMAASSVVAMRRLQRDSTLSA
                     LSQAATALANALPGALSGARLQGSAGLAPFAAAVDGFCDAAAAGTSLRVSAIAADGSV
                     LGDSGSDPGKMDNHATRPEFAEALLGRPTTIIRRSATLGLEMAYAAAPIRYGGSVAGA
                     LRVAMGAPDLGDELAPFLALSLALATATIAATAVASRRIGAAITMPLRALVEAAGDWS
                     AGRLDRRVRHSGDPAFEPLADAMNAMAAELAARTASTERQQRELGAILDAMGEAVLST
                     DGDLAIRLANPRARELLGPGAQPGRGLLEATGNASLETLALRAAAGELGDRVELALYG
                     AEARQLLVSGAPLPLEGDRPGAVLVLSDITRLKRLERVRTDFVANVSHELRTPITLIK
                     GFAETLEADPSEAGRFIGIIKRHADRMAAIVDDLLTLARLEGPERGRLETREVLAGPL
                     IERAIESLGDKPARRRAIVAVDAPVGLSLRADEGLLEQALVNLLDNAVKYSPEGGEVN
                     VEAASDGDDVVFTVRDHGPGVPAKDAARLFERFYRVDRARSREFGGTGLGLAIVRHIA
                     LAHGGDASVATREGSGCAFTLRVPRRPPEATEADSVQPASPDGDTARTSS"
     gene            complement(6761..7459)
                     /locus_tag="PLQ29_00030"
     CDS             complement(6761..7459)
                     /locus_tag="PLQ29_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPG85058.1"
                     /translation="MAKAIVLIIEDDPDILELLSYTLGKEGYETTMAATGEAGLAAMR
                     ERAPDLVLLDVMLPGMDGFELLRKAKADPALARVPVLMVSARGEEADVVTGLELGAED
                     YVTKPFSPRVLVARARAALRRSGDRAGDTPEDGPVVRGPCAIDPARHELLIDGRPVDL
                     SATEFAMLELLMRESGRVFTRAQMLDRVRGHDYPVTDRAVDVQILSIRRKLGRHAELV
                     ETIRGVGYRFKDLP"
     gene            complement(7469..8125)
                     /gene="phoU"
                     /locus_tag="PLQ29_00035"
     CDS             complement(7469..8125)
                     /gene="phoU"
                     /locus_tag="PLQ29_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPG85059.1"
                     /translation="MMRMQFNDEMNRLHHDILAMGSRVEEDLRKALIAFKTSDAALAD
                     EVKADDELVNAMQMKIEDQAAMLIATQQPVARDLRELITIFKVTDNLERIGDHAVHLA
                     KTAKRLSNDPYPRTLERLELMAATGVAMLHDAVTAYLDQNEALAREAAARDDQVDAEH
                     KAFVREVLDYLTEHPDKAQQATKLIATSSFLERLGDHVTNLCEAVVFMVTGAHTELND
                     "
     gene            complement(8140..8955)
                     /gene="pstB"
                     /locus_tag="PLQ29_00040"
     CDS             complement(8140..8955)
                     /gene="pstB"
                     /locus_tag="PLQ29_00040"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPG85060.1"
                     /translation="MRLFKKSAPEANRLEATIGSGDSGQALEAKSLSIFYGEFEAVKR
                     LSIGFERKKVTALIGPSGCGKSTALRAFNRMNELIRGVRTEGEVSFRGVNVYAPDVDA
                     VQVRRWIGMVFQKPNLFSKSIYENIAWGARINGYQGDFDELVENSLKQVALWDEVKDK
                     LKESAYALSGGQQQRLCIARAVALKPEILLMDEPTSALDPISTAKIESLLDDLKKRYT
                     IIIVTHNMQQAGRVSDKTGFMLLGELVEYSDTKELFFNPKDPRTENYISGRFG"
     gene            complement(8955..10199)
                     /gene="pstA"
                     /locus_tag="PLQ29_00045"
     CDS             complement(8955..10199)
                     /gene="pstA"
                     /locus_tag="PLQ29_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPG85061.1"
                     /translation="MSGREDFAVNLASRHARGDRWRLMFMLSTLLAIVFLGALILTIV
                     DDAFGYVVMVDEVDQRSLVPDGERLESLDAEALRAVIDERLSPRRYRAIERERPFDER
                     TAADLYDIVLYEIVKPRVAETYTLFESLFARRSIVEETTLAHPDGELVFRSWVNMRFL
                     STSQTARPLTTGVLTAVKGSILTISITILFSFPVGIAAAIWLEEYAGDNRLNRFIQVN
                     IYNLAGVPSIIYGMLGLAIFVRGLERFTSGSAFGASTSGEVANGRTILSAGLTLAILI
                     LPVIIINAQEAIRAIPGSLRQSSLAVGATKWQTIWHHVLPASFDRILTGAVLAVSRAI
                     GETAPLVVVGASTFLTQDPTGVFSKFTTLPIQIYQWTSRPQAEFRHIAAAAIVVLLAL
                     MLSLNAFAIFTRNRLRRQKRFG"
     gene            complement(10196..11137)
                     /gene="pstC"
                     /locus_tag="PLQ29_00050"
     CDS             complement(10196..11137)
                     /gene="pstC"
                     /locus_tag="PLQ29_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPG85062.1"
                     /translation="MKPVQTARYPEEARGALSFGKRFRPHEAVILALLFFCAAVSILT
                     TLGIIVVLGAESSALFKEVSLWEFVSGTSWQPTIGEFGILPLVNATLMTSLAAMLVAL
                     PLGLGVAIYLAEYASERVRETLKPILEVLAGVPTVVHGYFALTVVTPALRGLFGQESI
                     EVYNTLSAGLVMGILILPLISSMCEDALSAVPRSLREAAYAMGATKLETSVTIIVPAA
                     FSGIAAAFILGLSRAVGETMIVALAAGAGPNLTLNPLKAAETMTGHIVRISGGDISYD
                     SMDYRSLFAIALVLFLATLALNMVSRSIVKKYREVYE"
     gene            complement(11191..12165)
                     /locus_tag="PLQ29_00055"
     CDS             complement(11191..12165)
                     /locus_tag="PLQ29_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HPG85063.1"
                     /translation="MKKVAMMVALSLVAASAFAQGKFVWLGKTSDDPDKMLPGVDPAQ
                     VVGTIITAGSSTVFPVSELIASMFKEDGFAGQVTIDSIGSGAGFERFTKTGETDIANA
                     SVAIKQSQIDAAKAIGRTPIAFRLGTDALAVVVSKKNTFAKDMTQAELKLAFSTAVYW
                     SDVRASWPKKEIKRFIPGTDSGTFDYFVEHLYRKDRKPILDAKNLQLSEDDNVLVRGV
                     SGDAYAIGFFGFAYYQENKDKLTVLSIDTVEANAANVDNGTYPLARPIFIYSDAKIMN
                     DKPQVAAFIAYYLTYVNEALKEVGYFPAPAAEQKKAKQAWLDAVKGRY"
     gene            12446..13384
                     /locus_tag="PLQ29_00060"
     CDS             12446..13384
                     /locus_tag="PLQ29_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85064.1"
                     /translation="MKKCMIAVTLAAAVMLVAASCAVEAPVDELAYEAQVDADFEAVV
                     ATLGTADAARAFAGFDEKYGTSLSVDFAEQLSAPRFSSGGTNYAELLDMPFAVDGAVY
                     LSGGGEGLVSSVIGWVAPKSLPGGYYHGAVLDLDKFDPNNLEAASLQTAIAKGAGYES
                     AYDWQRQVNACVLDPVFTVNRARLNSAQSAIDYYCDPDNTDMSYGFFEGYVNIFNLVE
                     KESNDWWYCTKTVWRMYDRYGIDVDSNSPRVDFSDSGLYSLVKAYYNARYFYSSSKAK
                     AAMAAYIADARDTIVMAEEIMLSPYLTKVYEAIRAE"
     gene            13392..14465
                     /locus_tag="PLQ29_00065"
     CDS             13392..14465
                     /locus_tag="PLQ29_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85065.1"
                     /translation="MRSTIDYAGTAGRRSRPPRRGLRAAASLSWSIVAAATAAALFIG
                     CSAAASSPGYPIFALGGRLRSSGPGAPVLADLPSGVKPRALAAAKLGPGVALAYDQGS
                     GRLLSLDGRGRAVRSWPLEAAEAWVSDGLVLTRSAAFIEGRGFRFALYRVSGIGPERL
                     WDGELDCFPSDVAFAAGRAYLAGGDRADAELWLWELSERGAEPLYSRPKRSDFLRVTP
                     VGGRLLAFESQRVRERRPCVVRIVGDSPGGSPGETRDAPIVGLPGDALCLYGYGFEYG
                     GRLVVPVALEGGDLGLAALVDAGGQFEVASLARGAKGVYLPLGPGPDGAYRYLAYDYE
                     GDPTRRYLGRYDGSAVSLEFLGP"
     assembly_gap    14585..14684
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14695..15660)
                     /locus_tag="PLQ29_00070"
     CDS             complement(14695..15660)
                     /locus_tag="PLQ29_00070"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPG85066.1"
                     /translation="MMAAAKPASAAGPGAAADLSVTIRGMTMPNPVGVASGTFGYGEE
                     YAPLVDLDALGAIYTKAVTPEPRPGNPAPRLADTPAGMLNSIGLANPGLDAFVADKLP
                     LLRGRRCAIIVNVAGKTEDDYLRVVERLDGEAGIDGYEINVSCPNVAHGGLAFGTDPA
                     QVERLTRRLADARKAAPGRPIIVKLSPNVTDLTAIAKAAEAGGADALSCINTLVGMVI
                     DVKARRPTLAMGTGGLSGPAVRPVGVACVYRVSRAVSIPVIGLGGIARADDALQYLLA
                     GASAVQVGTALFSRPETPLEVLSGIRAFMEREGVERVGDIRAMLR"
     gene            complement(15657..>16226)
                     /locus_tag="PLQ29_00075"
     CDS             complement(15657..>16226)
                     /locus_tag="PLQ29_00075"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPG85067.1"
                     /translation="RGRGTEYLAALRPGDGLDVLGPLGRGFAKPPRGARPMLVAGGIG
                     VGPMLYLAATLATDAAAGLCEAPILVVGARTAALVPAAMLPAGTAVCTDDGSAGFSGT
                     VTDWLASFDPGLPPAFYACGPTPMMAAVAAVAASRRAPYEASVEQWMACGVGACAGCA
                     VRLRSGEYAKACSDGPVLDGSTVDWEAMA"
     assembly_gap    16227..16326
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16327..16606)
                     /locus_tag="PLQ29_00080"
     CDS             complement(<16327..16606)
                     /locus_tag="PLQ29_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85068.1"
                     /translation="MRRFDTIVTGNRPVADGYRELRFAWPHDDGRRADDPVPGRFFTL
                     RAGGRYDPALRRPFAFSDWRPAGDAACVFQLRGRGTEYLAALRPGDGLD"
     gene            16712..17407
                     /locus_tag="PLQ29_00085"
     CDS             16712..17407
                     /locus_tag="PLQ29_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85069.1"
                     /translation="MHRTYRNGPPSAVAFLAILFASAASAAAQDVGWAVSAACGPAVA
                     FGGALGDELAAFEDIAGADARYATVFGAGLAGGADIGLTGPLRAAASIGLSRLGYAFW
                     SPDAAALSYLSLWTAGVRLGLRLEPGDWLVGAGASLLFPVGPIVQYASLGGASVASHS
                     EATTASTMIPGAYIEGSLALGRPFAIGAALARPRLGLELGLWPGGLVEDSEAWQFALS
                     AVVGLDLERKAAR"
     gene            17404..>18452
                     /locus_tag="PLQ29_00090"
     CDS             17404..>18452
                     /locus_tag="PLQ29_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85070.1"
                     /translation="MKRLVMLVVSIAAASILASCDLFSGLAGGAAGTDDAPFVYFAES
                     SGGSIDLSLSAAAGRSASVVFTTGPYEGLAAPTATARDSSDVPAAASRLSAQDAFSDR
                     LRSYARENANAYIPPIRPRFSTTGDPAHDVAGATPSEASFYVIANSSSSVDFDVISSS
                     CRAVRDDTTFSDTRTRTLSVWVADDCWDLGSTKKHAVNQTMVDAIAEKFFGASSDPDT
                     SIYAWVTGMLGDEWGVHDESTRIGFGTYDLIAKSDNITILLADIGSDDADDGGIVGYF
                     YPVDTFAGADNSNERIMFIIDAVMYSNPDDSGISSNEPGYSDNGWSDEDYWAEQVFST
                     LAHEFQHMIHFYQKGV"
BASE COUNT         2809 a         6032 c         6203 g         3198 t
ORIGIN      
        1 ggacgcgtat gctcgggaag ccgaggctta agaggaagga ttcccccggg tcgcgcgcgg
       61 ctccagggga cggcctatcg tcgggcatca ctccaggtcc tcctcgtcgt cgtccgctag
      121 gggcagggcg cgcttgacag atacgagggc ggtgccgacg ccgaggccga tgaggccggc
      181 cggcaggaag ccgccgccga gcctcgaggc tacgaagcgc aggtagtcca gcaatgccgg
      241 cagctcggcg accgcgattt ccggtgatag cctgagcgct tgcccgggga ggatcagcct
      301 gggtaggagg ccgatggaca ggattatggc gctcggcgcg atgacgcggg agccgagcgt
      361 gcccaggcgc cgtcgccagt ccccatccga caccatgacg ctggcgaagc atagccccgc
      421 gccgctcagg agcagccata gcgacgcctg cgacgcggcg agcgaccagg agaccgatgg
      481 gatctccatc ctcgagcctg cctcgccgat cagcgcctgg tcaggctgcg cggcgagtgc
      541 gccgagcagc accgcctcga cggcggcctt gccggcgtct cccggcggta gcgtgatcgg
      601 cgtctccgcc cgacccggat cgcccggagc ccttgtcacg gtttccggta gcgcggcctt
      661 ggcgacgtag accgaggcga aggtcggcgc gccggtactg acggccgcct tgagcggcct
      721 cgcgtcgaag tagaagaaac cgtcgccgcg gccgaaagcc tcgtaggcag cgtcgatcgc
      781 gcccgagacg gtgtcgacaa cggcgctcgt cggcacggag gcctggaacg ccgcggtcgc
      841 ggccttgccg ttcagcacgg agccgccgag gcgcatggtc tcgggcgcca tcgtcaccag
      901 gtccggcgat cgtagtagct cggagaagcg cgggtcgacg accagggtct tgagcgcggc
      961 gctgtcgccg gccgccggcg cgagcgaggc taccgccagg ccgccgagca tcagcgggaa
     1021 agccatgcag aggtagaggg cgaagcccag gaagcgtctg agtcccttca tcgggcggta
     1081 cctccggcga gagcgacgta gggcctggcc ttgggggcgc cgaagcccat ggacttgaac
     1141 gggtggatat cgagcgggtt ctgaaaccag ccggtgacgg cgtcgaggtc gatgacgttg
     1201 aacgataccg agtggtagag cggtaagagg ggagcgtcgg acagtagctt ggcctccgcc
     1261 tgggcaagcg tcgcgagtcg ggccttgccc tcctcttcca tcgagcgggc catcagcgcg
     1321 tcgtactcgc ggctgcggta gccgccctcg ttcaagccgg agtcggcggt ccacatgagg
     1381 aggaaggccg ccgggtcggc gaagtcgcct atccagctcg tgaaggacag gtcgaagcca
     1441 gcctgccggt ggtcgcggat agactcgccg gcggggaccc gggcgcgctc gacgcgcaca
     1501 cctatagcgt tccaggcctc ctctatgacg gccaggttgg cttcgtgggt ctccgactcg
     1561 tagtagacga agcgtagcgc gggcaggccc tcgccgccgg ggtggccggc ctgagccagg
     1621 aggcgcatcg cctcctcctc gtcgcggcgg tcgatgccgg ctggagactc gtagccggcg
     1681 aagggcagca cgagggagct cgacggcgcg tagtagttgt cctcggtgcg tatcctgtcc
     1741 cagggtacga gcaaggcgag ggcccggcgg agccgcgcgt cgttccaggg ccgcctggac
     1801 gagttccaga agtagtagcc ggtggcgaac atcggggcga actggatggc gtcggtcgat
     1861 accagcgcgt ccaggtccgc catgtcggtc agccagtgga tctcgccatc gtcgtagcga
     1921 cgcgtcgcgt ccttctccga gtccaggaac agcaccttga tggtctggac cgccacgtta
     1981 cccgcgtccc agtaggtagg gttccggcgc agtagcatcg agccctcgtc catggcctcg
     2041 atgacgtagg ggccgttccc gaccacggcc gccgcttccc aggtccgcgt cccgcgcagc
     2101 gagcggtgga ccggcacgaa ggcgctatgg cacaggagcc gcgtaaaata agccgccggc
     2161 gatgtgagct cgacgaccag ggtcctatcg tcgggcgcgc gcagcccgac tgactcgggc
     2221 gagctactct tgccggtccg atagtccttg gcgccctgta ttatatcgaa gaacacggcg
     2281 tactcggcct tggtccttgg cgacaggaga tagagccacg actcgacgaa gtcgccggcc
     2341 gtgaccggcg tgccgtcggc ccagcgggcg ccagcccgca ttctgaaggt ccaggtcttg
     2401 ccatcggcgg acttctcgaa ggactccgcc tgggcgcgta ccgggtccag actctgcgga
     2461 tcgtagctga acagcccctc gtatagcgcg gtaaacagct gcatttcgtg ggcgtaaatg
     2521 gacttatacg ggttcagctc gacgtcgtag gttcccatgc ccacgacgag ctcccgggcg
     2581 gcgccatcgc tgaagacgag cgccggcgcg catacggata gcgcagccat gcatagccag
     2641 gcgctcttga gtcgatacag cttcatcatc gctcctcggt gggtcggcgg aacgcgcgcc
     2701 taggcttccc ggatgcccaa gcgtttcatc tgctcgagct cttccttctg cgctatgtac
     2761 tcatgccgct tctggttcga tttgattata gcgttcttga tggcgcccaa ctcgattttg
     2821 acgctacgga cgcgcccctt gaactcggga tcctcgatcg cgccttggaa gaactcgtcg
     2881 agggctgata gcttgcggtg gaaggcctgt agctcctcga tgctcttgga caggaacttg
     2941 tagatgacgt cctccgagga gccttccatg cgcttgaacg cggcgctcga cttggagacc
     3001 atcgccgcca ggctctgcgc ccgcttggcc gcctcgcggc agaatgccgt gaagtctatc
     3061 gactcgttct tgcgctcgct ggtcaccgga tcgaggtact cgacttcatg gaccacgtgt
     3121 tcgcccttct tgccgagcat ctcgcggatg gctcgcttga ccttggtcag gaacgagcgg
     3181 tccatcgatt cgagcagggc ctggttctcc tcgagcttgc gcacggcgtc ctcgagctga
     3241 aagccgacgc cgatcagcac ccgggcgccg tcgagcaggg cggccttgta gttgcgctcg
     3301 gccgacttgt ccccgctctt ggcctcggct actttgagag ccgccagaat cccgtcgcgc
     3361 agcgactcgc cctcggcgcc gtagtcttcc ttgagcgcct cggcgaccag ctccgcgtag
     3421 aaggggcgat cgccggcaac ttcagtgaat ttctgcctga ttttcctcac ggcgtcgtcc
     3481 tggtgggtca ccgcgtaatc acggtcgagg gccaggccgt tcatcacgag ccgcctgagt
     3541 tccagcttgt agcgctcctt atggatcaac gtcagagcct tgagggtctg gaatatctta
     3601 cggctcgcct tgtcgagctg caggagcccc tcgttgatga taccggtcga cagcgggtcg
     3661 gtacccttct tgacgagccc gactatctcc gcgaagtagc gcgtattgat atgctggctg
     3721 ttttcggtga actgcgtgaa cgcgaagtac ttggtcagcg aagccaggcg tttgatgcgg
     3781 cccatcgtca ggaactcgac gctgaactgg tagtaattat tgaggaagtc caggtacgac
     3841 tcgtactggg acagccgtat cgatatctgg tcgatcttct cgctctcggc gaaggggctc
     3901 tccgaggggg tcgtcacctc ggatatcttg aggtcgtact tatacggatc ctcgcttatg
     3961 aggcctttct tgaggagtac gttatagata gcctgaaacg ccgactggaa cagcttgaag
     4021 tcgtccttga gctgcttgag ttccgagcca tccatatgtt cgatcttggc ggacagggcc
     4081 cgctcgagcc tgtccgcgta cacaacgctg tcttccatcg ttgcgcacag tatggaataa
     4141 aaccgccgcg gaggcaagct cccatcgcca gctcgacgat atgcgggtcg caagcgcgga
     4201 ggcccgcgcc cgcgtgtacg cggcatggaa ccaacgcttt cgtacaggct attcgcggct
     4261 cggtttgcgc cggcccgcct attcgccgtc gcggccgccg ccatggccgc gttcgtcgct
     4321 tgccaggcgg cctctgagat ggaggcggcg atacgcttgc ctaccatgcc gtcggcctgg
     4381 gccggtttcg gcggagcccc tggagccggg ctctgggagc tgtcctggct gtcggcctca
     4441 ggctccgggg gaccgttccc ggcggcgcca ggcgcggtcg tcgcgcttcg cctgcctcgc
     4501 ggcgaggagg cagcggtctt ctgccgggcc gtcctggggg cggagcggag cctgccctat
     4561 ggagcggcct ggccccaggn nnnnnnnnnc ttcgcggccg aagccgccgc ccgcctctat
     4621 gggctcgggc tacggtactc cgggctggac ctcgtacggc tcgcctcgga ggcggaggcc
     4681 aggctcgccg atccctggga catggatccc gccgagctgg cgtcggccgc gctagaaggg
     4741 cgcctgcggg cggaccacct gcgcgagccg gaacgtgtcg agctgaccgt gaccgggcta
     4801 cccgggccgg cttggccaga cagcccctac ggaccccggg tcgagccgag gcccgacggt
     4861 acggcgaccg tctcgctacc ggtcggtcgc gtcaggcgct ggattggcga cggttacgag
     4921 ctcgtcctag cggtatcgcc gtcgggggag gcgggctgga cgctgtcggc ctctgtagcc
     4981 tccggagggc gcctcgggac gcgcaacgtg aaggcgcagc ccgagccctc gcgggtggcc
     5041 accgatgcgt cgccaccatg agccaacgcg atgtgcctga ctatggccag cccgaggccg
     5101 gtgccgccga actcgcggct gcgggccctg tcgacgcggt agaagcgctc gaacaggcgg
     5161 gccgcgtcct tggcaggcac gcccgggcca tggtcgcgta ccgtgaagac cacgtcgtca
     5221 ccatcggacg ccgcctcgac gttcacctca ccgccctcgg ggctgtactt caccgcgttg
     5281 tcgagcaggt tgacgagggc ctgctcgagg aggccttcgt cggcccggag cgatagcccg
     5341 accggcgcgt cgaccgcgac gatcgcgcgc ctgcgggccg gcttgtcgcc gagcgactcg
     5401 atggcgcgct cgatgagcgg cccggccagg acctcccgcg tctcgagccg gccgcgctcg
     5461 ggaccctcga gtcgggcgag ggtcaggaga tcatcgacga tcgcggccat gcggtccgcg
     5521 tgccgcttga tgatgccgat gaagcggccg gcctcggacg ggtccgcctc gagggtctcg
     5581 gcgaagccct tgatcagggt gatgggcgtg cgtagctcgt gcgagacgtt cgcgacgaag
     5641 tcggtacgta cccgctcgag gcgcttgagc ctagtaatat cgctgagtac gagtacggcg
     5701 cctgggcggt cgccctcgag cggtaagggc gcgccgctga ccaggagttg gcgcgcctcg
     5761 gcgccgtaga gcgccagttc gaccctgtca ccgagctcgc ccgcggccgc cctgagagcc
     5821 agagtctcga gcgaggcgtt gcccgtcgcc tcgaggaggc cccggccggg ttgggcgccg
     5881 gggccgagca actccctggc ccgggggttg gccagccgga tcgccaggtc tccgtcggtc
     5941 gacagtaccg cctcgcccat cgcgtcgagt atggccccga gctcgcgctg ctgccgctcg
     6001 gtcgaagccg tccgcgccgc cagttcggcg gccatcgcgt tcatcgcgtc ggcgagcggc
     6061 tcgaaggccg gatcgccgga atgccggacg cggcgatcga ggcggcccgc cgaccagtcg
     6121 ccggcggcct cgacgagggc gcggagcggc atggtgatcg ccgcgccgat gcgtcggctc
     6181 gcgaccgccg tggcggctat ggtagcagtc gctaacgcga gcgacagcgc taggaagggc
     6241 gccagctcgt cgccaaggtc gggcgcgccc atcgccaccc ggagggcgcc ggctaccgac
     6301 cctccgtatc gtatcggggc ggcggcatag gccatctcga ggcccagtgt cgccgatctc
     6361 ctgatgatcg tggtaggcct accgagcagg gcctcggcaa attctggcct ggtcgcgtgg
     6421 ttatccatct tgccggggtc ggagcccgag tcgccgagca ccgagccatc ggcggcaatg
     6481 gccgagacgc gaaggctcgt gccggccgcg gccgcgtcac agaagccgtc cacggccgcc
     6541 gcgaagggcg ccaaaccggc acttccttgt aaccgggccc cggacagcgc gccgggcaag
     6601 gcgttggcca acgcggtggc tgcctgggac agcgccgaca gggtcgagtc gcgctgcagg
     6661 cggcgcatgg cgactaccga gctggccgcc ataatcgcga ccgcggccgc cgataggagg
     6721 aacagcgccg tgaaggtcct cgtccccggg cgggcgcgcc tcacggcagg tccttgaagc
     6781 ggtagccgac gccgcgtatc gtctcgacga gctccgcatg gcggccgagc ttgcggcgta
     6841 tcgataggat ctgcacgtcg acggcgcggt cggtcaccgg gtagtcgtgg ccgcgcaccc
     6901 ggtcgagcat ctgagcgcgg gtgaacacgc ggccgctctc gcgcattaaa agctcgagca
     6961 tcgcgaactc ggtcgccgac aggtcgaccg gccggccgtc gatgagcagc tcgtgcctgg
     7021 cagggtcgat ggcgcagggg cctcggacta cggggccgtc ctcgggcgta tctccggccc
     7081 ggtcgcccga gcgcctgagg gccgccctgg cccgcgccac gagcacgcgc gggctgaacg
     7141 gcttggtcac gtagtcctca gcgccaagct cgaggccggt caccacgtcc gcctcctcgc
     7201 cgcgcgcgct gaccatcagc accggcaccc tggccagggc cgggtccgcc ttggccttgc
     7261 gcaacagctc gaagccgtcc atgccgggta gcatcacgtc gagcaggacg aggtcgggcg
     7321 cgcgctcccg catcgcggcg aggcccgcct cgccggtcgc ggccatcgtc gtctcgtagc
     7381 cttccttgcc gagcgtgtag ctcaggagct cgaggatgtc gggatcgtcc tcgatgatga
     7441 gtactatagc cttggccatg cggcgctcct agtcgttcag ttcggtatgc gccccggtca
     7501 ccatgaagac caccgcctcg cagaggttcg tgacgtggtc gcccaggcgc tcgaggaagc
     7561 tggacgtggc tatcagcttg gtcgcctgct gcgccttgtc aggatgctcg gtcaggtagt
     7621 cgagcacttc ccgcacgaag gccttatgct cggcgtctac ctggtcgtcg cgcgctgccg
     7681 cctccctggc cagcgcctcg ttctgatcga gataggccgt gacggcgtcg tgtagcatgg
     7741 ccacgccggt cgccgccatc agctcgagcc gctccagggt gcgcgggtag gggtcgttgg
     7801 acaggcgctt ggcggtcttg gccaggtgga cggcatggtc gcctatacgc tccaggttgt
     7861 ctgttacttt gaagatggtg atgagctcgc gcaggtccct ggctaccggc tgctgggtcg
     7921 ctatgagcat cgcggcctgg tcttctatct tcatctgcat ggcgttgacc agctcgtcat
     7981 cggccttgac ctcgtcggcc agggccgcgt ccgacgtctt gaacgcgatc agggccttgc
     8041 ggagatcctc ttcgacgcgg ctacccatgg ccaggatgtc gtggtgcaag cggttcatct
     8101 cgtcgttgaa ttgcatgcgc atcatggaag cctccgttat cagccgaagc gacccgagat
     8161 gtagttctcg gtgcgcgggt ccttgggatt gaagaagagc tctttggtgt cggagtactc
     8221 tacgagctcc ccgaggagca tgaagccggt cttgtccgag acccgccccg cctgctgcat
     8281 gttgtgcgtt acgatgatga tagtatagcg cttcttgaga tcgtcgagca gcgattctat
     8341 cttggcggtc gatatcgggt ccagcgcgct cgtcggttcg tccatcagga ggatctccgg
     8401 cttgagcgcg acggcgcgcg ctatgcagag ccgttgctgc tgcccgcccg atagcgcgta
     8461 ggcgctctcc ttgagcttgt ccttgacctc gtcccagagc gcgacctgct tgaggctgtt
     8521 ctcgacgagc tcgtcgaagt cgccctggta accgttaatc ctggcgcccc aggctatgtt
     8581 ctcgtagatg ctcttggaga agaggttcgg cttctggaat accatgccga tccatcggcg
     8641 cacctggacc gcgtcgacgt ccggggcgta tacgttgacg ccgcggaagc tgacctcgcc
     8701 ttcggtccgg actccgcgta taagctcgtt catccggtta aaggcgcgga gagcggtgct
     8761 cttgccgcag ccggatgggc ctatcagcgc cgtgaccttc ttgcgctcga accctatcga
     8821 caggcgcttg accgcctcga actccccgta gaagatggag aggctcttcg cctcgagcgc
     8881 ctgcccggag tcccccgatc ctatcgtcgc ctcgagccgg ttcgcctctg gagcgctctt
     8941 cttgaatagt ctcatcagcc gaacctcttc tgtctgcgca agcggttgcg cgtgaatatc
     9001 gcgaaggcgt tgagcgacag catgagcgcc agcagtacga cgatggcggc cgcggctata
     9061 tgcctgaact cggcctgcgg cctgctcgtc cactggtaaa tctgtatcgg cagggtcgtg
     9121 aacttcgaga atacgcccgt ggggtcctgc gtcaggaagg tcgacgcgcc gacgacgacc
     9181 agcggcgccg tctcgcctat ggcgcgcgag accgccagta cggcgccggt gagtattcgg
     9241 tcgaagctcg ctggcagcac gtggtgccat atcgtctgcc acttggtcgc gccgacggcg
     9301 aggctcgact gtcgaagcga gccgggtatc gcgcgtatcg cctcctgcgc gttgatgatg
     9361 atcaccggca gtatgaggat cgcgagcgtc agcccggccg agaggatggt ccgcccgttg
     9421 gcgacctcgc cggatgtcga cgcgccgaag gcgctgcccg aggtgaaccg ctccaggccg
     9481 cggacgaaga tggcgaggcc gagcatgccg tagatgatcg agggcacgcc ggccaggttg
     9541 tagatgttca cctggatgaa gcggttgagc cggttgtcgc ccgcgtattc ctccagccag
     9601 atcgcagccg ctatgccgac cgggaacgag aagagtatcg tgatcgatat cgtcaatatg
     9661 gatcccttga ccgccgtcag cacgccggtg gtcagcggcc tggcggtctg cgacgttgac
     9721 aggaagcgca tgttcaccca ggagcggaag accagctccc catcggggtg ggctagagtc
     9781 gtctcctcga cgatgctcct cctcgcgaac agggattcga acagggtgta cgtctcggcg
     9841 acccgcggct tgacgatctc gtacagcacg atgtcgtaca gatcggctgc ggtccgctcg
     9901 tcgaacggcc gctccctctc gatcgcgcgg tagcgacggg ggctcagccg ctcgtcgatg
     9961 acggcgcgca aggcctcggc gtccaggctt tcgagcctct cgccgtccgg tacgaggctc
    10021 cgctggtcga cctcgtcgac catgaccacg tagccgaacg cgtcgtcgac gatggtcagt
    10081 atcaacgccc cgaggaacac aatggcgagc agggtcgaca gcatgaacat gagccgccaa
    10141 cggtcgcccc tcgcgtgcct cgaggcgagg ttgacggcga agtcctcgcg gccgctcatt
    10201 cgtacacctc gcggtatttc ttgactatgc tcctggagac catgttgagc gcgagcgtcg
    10261 cgaggaacag cacgagcgct atcgcgaaca ggctgcggta gtccatcgag tcgtaggata
    10321 tgtcgccgcc ggagatcctg acgatgtggc cggtcatcgt ctcggcggcc ttgagcgggt
    10381 tgagcgtgag gttcggcccc gccccggccg cgagcgcgac gatcatcgtc tcgcctacgg
    10441 cgcgcgacag gccgaggatg aaggccgcgg cgatcccgga gaaggccgcc ggcacgatga
    10501 tcgtcacgct ggtctccagc ttggtcgcgc ccatcgcgta ggccgcctcg cggaggctcc
    10561 gcgggacggc ggagagcgcg tcctcgcaca tcgagctgat cagcggcagt atcaggatgc
    10621 ccatcacgag cccggccgat agggtgttgt agacctcgat cgactcctgg ccgaacaggc
    10681 cgcgcaacgc gggcgtgacg accgtcaggg cgaaataccc gtgcacgacg gtaggtacgc
    10741 ctgcgagcac ctcgagtatc ggcttcagcg tctcgcggac gcgctcggac gcgtactcgg
    10801 ccaggtagat ggccacgcca aggcccaggg gcagggcgac gagcatcgcc gcgaggctgg
    10861 tcatcagcgt cgcgttgacg agcggcagga tgccgaactc cccgatcgtg ggttgccagg
    10921 acgtaccgct gacgaattcc cagaggctca cttccttgaa caaggccgac gactcggcgc
    10981 ccagcaccac gatgatgccg agcgtcgtga ggatggatac cgcggcgcag aagaacagga
    11041 gcgccaggat gacggcctcg tggggccgga agcgcttccc gaaggaaagc gcgccgcgcg
    11101 cctcctccgg gtacctcgcc gtctggactg gtttcatcgt tctcgctttc agctggagcc
    11161 gcgcgccctc gctatgaacg gggcttcgtt ttagtagcgg cccttgacgg cgtccagcca
    11221 tgcctgcttg gccttcttct gctccgccgc gggggcgggg aagtaaccga cttccttgag
    11281 cgcctcgttc acgtaggtca ggtagtaggc gatgaaggcg gccacctgcg gcttgtcgtt
    11341 catgatcttc gcgtcgctgt agatgaagat cgggcgggcg agggggtagg tgccgttgtc
    11401 gacgttagcc gcgttggcct cgaccgtgtc gatcgacagc acggttagct tgtccttgtt
    11461 ctcctggtag tacgcgaaac cgaagaagcc tatcgcgtag gcgtcgcctg agacgccgcg
    11521 gaccagcacg ttgtcatcct cggaaagctg gagattcttc gcgtcgagga tcggcttgcg
    11581 gtccttcctg tacaggtgct cgacgaagta gtcgaaggtg ccggaatcgg taccggggat
    11641 gaagcgcttg atctccttct tgggccagct ggcgcggacg tcggaccagt agaccgccgt
    11701 ggagaacgcg agcttgagtt ccgcctgggt catatccttg gcgaaggtat tcttcttgct
    11761 gacgacgacg gcgagggcgt cggtgccgag ccggaacgcg atgggggtgc ggccgatcgc
    11821 cttggcggcg tcgatctggc tctgcttgat ggcgaccgag gcgttcgcta tgtcggtctc
    11881 gccggtcttg gtgaagcgct cgaagccggc gcccgatccg atcgagtcga tcgtgacctg
    11941 cccggcgaac ccgtcttcct tgaacatcga cgcgatcagc tccgataccg ggaataccgt
    12001 cgagctgccg gcggtgatga tggtgccgac gacctgagcg gggtcgacgc cggggagcat
    12061 cttgtccgga tcgtcgctgg tcttacctaa ccagacgaac ttgccctgcg cgaaagcgct
    12121 agccgcgact aggctcagcg cgaccatcat agcgaccttc ttcatctgtt ccctccagtg
    12181 gcgtagattg cggtgttcat tctccgcatc gggcctgagg atacggccgt gccgttcgcc
    12241 tacgatgagg cctttgttaa gaaacggtta atcgcgccgc ggaacggcgg tggggccccg
    12301 gggcttacgc gtgattgaca acgggctaaa acggtttact ctagtcgtgg ctcgctagaa
    12361 gcgatccgcc gttggcgatc cgccgttggc gatccgccgt tggcgatccg cgctcctggg
    12421 cctatccagt aaacggagga agtcaatgaa gaaatgtatg atcgcggtca cgctcgccgc
    12481 ggcagtcatg ctcgtagcgg cctcgtgcgc cgtcgaggcg cctgtcgacg agctcgccta
    12541 cgaggcccag gtcgacgccg atttcgaggc cgtcgtcgcg acgctcggca cggccgacgc
    12601 cgcccgcgcc ttcgccggct tcgacgagaa gtacggtacc tcgctgtccg tcgacttcgc
    12661 cgagcagctg tcggccccgc gcttcagctc gggcgggacg aactacgccg agctcctcga
    12721 catgccgttc gcggtggacg gcgctgtcta cctgtccggc ggcggagagg gcctcgtcag
    12781 ctccgtgata ggctgggtgg cccccaaaag tctgccgggc ggctactacc acggcgccgt
    12841 gctcgacctc gataaattcg acccgaacaa cctcgaagcc gcgagcctcc agaccgccat
    12901 cgccaagggc gccggctacg agagcgccta cgactggcag cgccaggtca acgcctgcgt
    12961 gctcgacccg gtcttcaccg tcaatcgggc ccgcctcaat tccgcccagt cggcgatcga
    13021 ttactactgc gacccggaca acaccgacat gtcctacggc ttcttcgagg gctacgtcaa
    13081 catcttcaac ctggtggaga aggagtcgaa cgactggtgg tactgcacca agaccgtctg
    13141 gcgcatgtac gaccggtacg gcatcgacgt cgactcgaac tcgccgcgcg tcgacttcag
    13201 cgactcgggc ctctatagcc tggtcaaggc ctactacaac gcccgctact tctatagctc
    13261 cagcaaggcc aaggccgcga tggccgccta catagccgac gcccgcgata ccatcgtgat
    13321 ggccgaggag atcatgctgt ctccgtacct gaccaaggta tacgaggcca tcagggccga
    13381 gtgacagggg cttgaggtcg acgatagact atgctggaac ggcgggtcgg cggagccgcc
    13441 cgccgcgccg cggcctgagg gccgcggcgt ccctgtcatg gtctatcgtc gcggccgcta
    13501 ccgcggccgc gctcttcata ggatgctccg ccgccgcttc gagccccggc tatcccatct
    13561 tcgcgctcgg cggccgcctt cgctcctccg gcccaggcgc tccagtgctg gccgacctgc
    13621 cgagcggcgt caagccgcgg gccctggccg cagcgaagct cgggcctggc gtcgcgctcg
    13681 cctacgacca gggctcgggc cggctcctgt cgctcgatgg tcgaggccgc gccgtgcggt
    13741 catggccgct cgaggccgcc gaggcctggg tctcggacgg ccttgtcctg acccgttcgg
    13801 ccgccttcat cgagggtcgg ggctttcgct tcgcgctcta ccgcgtctcc gggatcggac
    13861 ccgagcgcct ctgggacggc gagctcgact gcttcccgtc ggacgtagcg ttcgcggccg
    13921 gccgcgccta cctggccggc ggcgaccgcg ccgacgccga gctctggctc tgggagctgt
    13981 ctgaacgggg cgccgagccg ctctactcgc ggccgaagcg atccgacttc ctgcgcgtca
    14041 caccggtcgg ggggcgactc ctggctttcg aaagccaacg ggtcagggaa cggcggccct
    14101 gcgtcgtgcg gatcgtcggg gactcgcccg gcggctcgcc gggcgaaacc cgcgacgcgc
    14161 ccatcgtcgg gctgcccggc gacgcgctct gcctgtacgg ttacggtttc gagtacggag
    14221 gacggctcgt cgtgccggtc gccctggaag gcggcgacct cggcctcgcg gccctcgtcg
    14281 acgcgggcgg tcagttcgag gtcgcgagcc tggctcgcgg ggccaagggc gtctacctac
    14341 cgctgggccc gggcccggac ggcgcctatc gctacctggc ctacgattac gagggcgacc
    14401 cgacgcgccg ctacctcggc cgctacgacg gctcggccgt gagcctcgag ttcctcgggc
    14461 cgtgaacgag gaccccaggc gccgtcgagc gccgtagcat cgtcgctacc gcagcatcgt
    14521 cgctaccgca gcatcgtcgc taccgcagca tcgctcggat atctccgacg cgctcgacgc
    14581 cctcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagcatc gtcgctaccg
    14701 cagcatcgct cggatatctc cgacgcgctc gacgccctcg cgctccatga aggcgcggat
    14761 gcctgacagc acctcgagcg gggtttcggg cctggagaac agcgccgtgc cgacctgcac
    14821 ggcgctcgct ccggcgagca ggtactggag cgcgtcgtcg gcgcgggcga tgccgccgag
    14881 gcctatcacc gggatcgata ccgcccggct gacccggtag acgcaggcga cgccgaccgg
    14941 gcgtacggcc ggcccggaca gtccgcccgt gcccatcgcg agcgtcgggc gcctggcctt
    15001 gacgtctatc accatgccga cgagggtgtt gatgcacgac agcgcgtcgg cgcctccggc
    15061 ctcggcggcc ttggcgatgg ccgtgaggtc ggtgacgttc ggcgatagct tgacgatgat
    15121 gggtcggccg ggggcggcct tgcgcgcgtc ggccaggcgc cgcgtcaggc gttccacctg
    15181 ggccggatcg gtgccgaagg ccaggccgcc gtgcgccacg ttcgggcagg acacgttgat
    15241 ctcgtagccg tcgatcccgg cctccccgtc gagccgctcg acgacgcgca ggtagtcatc
    15301 ctcggtcttg ccagccacgt tgacgatgat ggcgcagcgc cggccgcgga gaagcggcag
    15361 cttgtcggcc acgaaggcat ccaggcccgg gttggcgagg cctatcgagt tgagcatacc
    15421 cgccggcgta tcggcgaggc ggggggcggg gttgcccggc ctgggctcag gcgtcaccgc
    15481 cttcgtatag atagcgccga gagcgtccag gtcgacgagg ggggcgtact cctcgccgta
    15541 gccgaaggtg ccgctcgcga cgccgaccgg gttcggcatc gtcatgccgc gtatcgtcac
    15601 gctcaggtcc gcggccgctc cggggccggc ggcgctcgct ggcttcgcag cggccatcat
    15661 gccatagcct cccagtcgac cgtgctcccg tcgagcacgg gtccgtcgga gcaggccttg
    15721 gcgtactcgc ctgaacgtag cctgacggcg catccggcgc aggcgccgac gccgcaggcc
    15781 atccactgct ctaccgacgc ctcgtagggc gcgcgcctcg acgcggcgac ggccgcgacc
    15841 gcggccatca tcggtgtggg gccgcatgcg tagaaggccg gcggcaggcc cgggtcaaag
    15901 gaggcgagcc agtcggtcac ggtgccgctg aagccggccg agccgtcgtc ggtgcagact
    15961 gcggtgccgg cgggcagcat ggccgccggc acgagggcgg cggtcctggc gccgacgacc
    16021 aggatgggcg cctcgcagag gccggcggcc gcgtcggtgg ccagcgtcgc ggccaggtac
    16081 agcatcgggc ccacgccaat gccgccggct accagcatcg gcctggcgcc gcgcggcggc
    16141 ttggcgaagc cgcggccgag cggaccgagc acgtcgaggc cgtcgcccgg gcgcagggcg
    16201 gccaggtatt cggtgccgcg gccgcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16321 nnnnnncgtc gaggccgtcg cccgggcgca gggcggccag gtattcggtg ccgcggccgc
    16381 gtagctggaa gacgcaggcc gcgtcgccgg ccgggcgcca gtccgagaag gcgaaggggc
    16441 ggcgcagggc cgggtcgtag cggccgccgg cccgaagcgt gaagaagcgg ccggggaccg
    16501 gatcgtccgc gcggcggccg tcgtcgtgcg gccacgcgaa acgtagctcg cggtagccgt
    16561 cggctaccgg cctgttcccc gttacgatcg tatcgaatct gcgcatggtc cgtaccgctc
    16621 ccgtgccgtc tggatcgccg ccagcgtagc ggtgcgggcg ggcgctgtca acgatgcatt
    16681 atcgtcgcct ttgtgctaga ttccagctac tatgcatcga acgtaccgta atgggccgcc
    16741 ctcggccgta gcgtttctag ccatactgtt cgcgtccgcg gcttcggccg ccgcgcagga
    16801 cgtcggatgg gccgtatcgg cggcctgcgg gccggccgtc gccttcggcg gcgcgctggg
    16861 cgacgagcta gccgccttcg aggacatagc cggcgcggac gcccgatacg cgaccgtgtt
    16921 cggcgcgggc ttagcgggcg gcgccgatat cggccttacg gggcctctgc gagcggccgc
    16981 ctcgatcggc ttgtcgcgcc tgggctacgc gttctggtcg cccgacgccg ccgcgctgtc
    17041 atacctgtcg ctgtggaccg ccggcgtacg gctgggtctg cgcctggaac caggcgattg
    17101 gctcgtcggc gccggcgcct cgctcctgtt cccagtcggt cctatcgtcc aatacgcgtc
    17161 gctcggcggc gcgtccgtag cgtcgcatag cgaggcgacg accgcgagta ccatgatccc
    17221 gggcgcctat atcgagggct ccctggcgct cggccgcccg ttcgccatcg gcgccgccct
    17281 ggcccgtccc aggctcgggc tggagctagg gttatggccg ggcggcctcg tagaggattc
    17341 tgaagcctgg cagttcgccc tgtcggccgt cgtcggcctc gacctggaaa ggaaggccgc
    17401 ccgatgaagc gcctcgtcat gctcgtcgtc tccatcgcag cggcctctat cctcgcgtcc
    17461 tgcgacctct tctccggcct cgccggcggc gccgccggta ccgacgacgc gccgttcgtc
    17521 tacttcgccg agtcgtccgg cggctccatc gacctgagcc tctccgccgc cgccggccgc
    17581 tccgccagcg tcgtctttac caccggcccc tacgaaggcc tcgcggcccc gaccgcgacc
    17641 gccagggact cgagcgacgt accggcggcg gcttccaggc tttccgctca ggatgctttc
    17701 tccgataggc tccgttccta cgcccgcgag aatgcaaacg cgtatattcc gccgatacgg
    17761 cctcgcttct cgacgaccgg agatcctgca cacgacgtcg ctggagcgac tccttcagaa
    17821 gctagtttct atgtcatcgc caattcctcg agctccgttg atttcgatgt aattagctca
    17881 agctgccgtg cggtgagaga cgatacgaca ttcagcgata ctaggacccg tacgctgagc
    17941 gtctgggtcg ccgatgattg ctgggattta ggttcaacca agaaacatgc tgtgaatcaa
    18001 accatggtcg acgccatcgc cgagaagttc tttggtgcct ctagcgatcc cgatacatcc
    18061 atctatgcat gggtcactgg catgctcggg gacgagtggg gcgtgcatga cgaatcgaca
    18121 aggatcgggt tcggaacgta tgatctaatc gccaagagcg acaatatcac catcttgcta
    18181 gcagatatcg ggagtgatga tgcggacgac ggtggcatcg tcggatattt ctatccggtc
    18241 gatacatttg ccggagctga taattctaac gaacgaatca tgttcataat cgatgcagta
    18301 atgtattcga atcctgatga ttccggtatt tcatcgaatg agccaggcta tagcgataat
    18361 ggttggtcag acgaggatta ttgggccgag caggttttct ccaccctcgc ccacgagttc
    18421 cagcacatga tccacttcta ccagaagggc gt
//
LOCUS       DAOPHV010000002        18075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_3434_length_18075_cov_4.880966, whole genome shotgun sequence.
ACCESSION   DAOPHV010000002 DAOPHV010000000
VERSION     DAOPHV010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 18075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18075
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3434_length_18075_cov_4.880966"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLQ29_00095"
     CDS             <1..410
                     /locus_tag="PLQ29_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HPG85071.1"
                     /translation="KRLMAYLADHPVAALPPGDPAWFEAHIMHPASAATGDYEVFIGA
                     EAERPLDAPVELVVKSLPARDYAVLSMRCAEMEPGWEDRVSAEVVGRYGRAIERRWGL
                     QRYDSRFSSLEDPGSIIEMWTPLAPETPDGEAH"
     gene            407..1255
                     /locus_tag="PLQ29_00100"
     CDS             407..1255
                     /locus_tag="PLQ29_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPG85072.1"
                     /translation="MTDADRRLASVGRALDLVEERLRDDLDVAALASAACYSIFHFVR
                     VFTELTGHGPYDYLMRRRVAEAARELVSGDRSILELALDYRFESPDGFSRAFRRCYGL
                     APSEARKAGAFPDGSARTPITREYVHASMNAGLTIRAERLSDVLVSGRSLAPGEAAGS
                     EARLMILGTGRDAFAGRAADEVPDYPTLGTRVPGGDCIVASGQPPLDLGLARERLYRT
                     AIPVGSARVRADFELIRLDSGGGPIAILVPVLLSEPLGVKMGRGDRWNDRPYAPESRI
                     RGPRLR"
     gene            1198..2304
                     /locus_tag="PLQ29_00105"
     CDS             1198..2304
                     /locus_tag="PLQ29_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85073.1"
                     /translation="MERQTIRAGESDPRAEAPVTIASLALLGCGTVGGALIDLIAERA
                     DALAARGVEPRVVAIANSRRMVVAPDGIDLARWREALAAASEPTDPSALMALGPSLPG
                     PVLVDCTASDETPRAYPGFFAAGFDVVTANKRGNAGPLEHYRNLAREARRRGRQYLYE
                     ATVGAGLPVVDNLRRLFMAGDSLLDFTGVLSGSLSFLFGRLEDGQLFSAAVREAMARG
                     FTEPDPRDDLSGADVARKTLLLAREAGMALEPEDVERAGLIPPEYLAMARDEFVARLP
                     ELDEGVAALTRSASGRGAVLRYAGSIAGGRASVGVVEAGPGHPLRSVRDGENALAFHT
                     RYYDPVPLVVRGYGAGALVTAAGVLGDVLRAAGH"
     gene            complement(2394..3563)
                     /gene="metK"
                     /locus_tag="PLQ29_00110"
     CDS             complement(2394..3563)
                     /gene="metK"
                     /locus_tag="PLQ29_00110"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPG85074.1"
                     /translation="MSDKRKLFTSESVGEGHPDKLCDQVSDAVLDACLRDDSESHVAC
                     ECFATTGMVLVGGEITTDTYVDIQTLVRDVVKEIGYTDPDYGIDYESMAILNTIHSQS
                     QDINQGVVGHGLKEYEGQQGAGDQGMMFGFACDETPELMPAPIMYAHKLLLEATALRK
                     SKAIPWLRPDAKSQVTLEYDGHKPIRAEAIVISHQHDPDVSYETIREAVIERIVRPVL
                     GPTGLLDDKTKFYVNPTGRFVVGGPMGDSGLTGRKIIVDTYGGMGRHGGGAFSGKDPS
                     KVDRSAAYMARYVAKNVVAAGLASRCEVQLAYAIGVPFPVSVMLETFGTAKADEEAIV
                     AAVRKTFDLSPAGIVSGLGLKAPIYRRTAAYGHFGRDGFPWERTDKVEALKAALR"
     gene            3861..5351
                     /gene="glpK"
                     /locus_tag="PLQ29_00115"
     CDS             3861..5351
                     /gene="glpK"
                     /locus_tag="PLQ29_00115"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPG85075.1"
                     /translation="MQHNDKYILALDQGTTSSRAIVFNQTGVVIGMKQIAFNQIYPKP
                     GWVEHDPFDIWQSQYDAAVGAIDEAQITPSQIAAIGITNQRETTLIWDRKTGKPVHNA
                     IVWQCRRSSRICQELKALGFEPEIKARTGLILDPYFSGTKLVWLFDQIPGLRDRAERG
                     ELAFGTVDSWLIYNLTGRHATDATNASRTMLFNIREGRWDQEILMMLKIPEAILPEVL
                     PSSADFGYTRKDLFGCEIPVCGCAGDQQAALFGHACFQVGASKNTYGTGCFALMNTGS
                     EPVESRHNLLTTIAWDLGKGYTYALEGSVFIGGAVIQWLRDEMRLLSDSAESERLARL
                     VKDTGGVVLVPAFVGMGAPYWDAEARGAALGVTRGTTKAHFVRAALESVAYQSRDLLD
                     AMERDAGASIPFIKADGGASANSFLMQFQADVLDKPVILPEVGEVTALGAAYLAGLRV
                     GYWSGLDELERNWRRKRDFMPEMAAETRDRLLERWSKAVEVTRLFK"
     gene            complement(5388..6983)
                     /locus_tag="PLQ29_00120"
     CDS             complement(5388..6983)
                     /locus_tag="PLQ29_00120"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPG85076.1"
                     /translation="MTLTKRVMVSVIGLLAWAASTATAQSVSQESGLEAPASATSPAD
                     PFATGYEAAVSAYRAAIDFSAPFEPVVDLPATGAVAGDGLTPWSLDELLALAAGRNPG
                     LRALAEAEAAAAADLAGARARRLPTLRAETSGTYIGNPMGPIALTAGQLGAFQGVSIP
                     PEDVIIYKGMESYNYDFALIGEVPLYTWGKIAIGVDLARAGLDAAGLQRRKAERELAA
                     RMRGDWDALACLRAADEMLGLQATIGRRLVELAEASAEAGFMTRADLAGARIKLKEID
                     IARVRLDERRDKLLAELASMAGLDALSKDDLEMTPPIAGSPRWTEAEAGALALGNSYD
                     LALLEAQLVARRGLRDLAEAEAKGRPDIGLRVELSYGGSRLPFVEKDWFGQDDYQLTF
                     SVGTSGNIFGNAVKEGEAAKARALLAEAAAQRADAERAIRSFVRDTWLGMELGRARLE
                     YAALRQEGWAADLAQKRAALRAGGGSESEYLTGMIEALGSLAEAYGTLAEYRGALVSL
                     DALAGSPSPAAPATAPMEAPTAP"
     gene            complement(6980..7294)
                     /locus_tag="PLQ29_00125"
     CDS             complement(6980..7294)
                     /locus_tag="PLQ29_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85077.1"
                     /translation="MAGRVVDDTDGITGINGVTVELTVKDREYSADSAPVDTTASTEN
                     GRFDLGTIVWEIGDGSGSTDWKVGEVDVTMTATGATLVSGKLDTLKPDTQRNVTLRVT
                     TP"
     gene            complement(7316..8275)
                     /locus_tag="PLQ29_00130"
     CDS             complement(7316..8275)
                     /locus_tag="PLQ29_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85078.1"
                     /translation="MSNLATTRRIAALIIIAVAAGLAASCSAVFRSSIQGSIIDLEDW
                     DDGTTTGVADAKLFLYTDDKARDADYASFVDGDESTLPDGSARTERGYFQSTVTDADG
                     GYDFTGFIWESLFPKYGKTADRDEVFFLIYHPDYGLWKNPTPLYVVSDVTNRLDYIKI
                     EDLWNEGRLAGTVLDWKDDKGLGGVTVNFYVAESWAYDASGGFTDIVYPTAPTATTSS
                     DADGAWVATVRFPMRPDRATHATHNNAPVRITYVLTNYRCNDDEGSTSFTNGTAVMLG
                     ADLDRDGKTAAQGDYEDAYVQATLEYDADEGEAVLGAAPTVTM"
     gene            complement(8268..11402)
                     /locus_tag="PLQ29_00135"
     CDS             complement(8268..11402)
                     /locus_tag="PLQ29_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPG85079.1"
                     /translation="MILSDFSVKHPAVVTILLAGLVVFGLIAGSSLNSEMIPPVGLPI
                     ATVLTVYPGAGAKDVERDITRKIENQLSSLAGVTEMSSSSADSYSTVSLEFRADVDVG
                     SKLPQIRELLNGIANDLPSGIEGAPVVFIAEASGFLPVFSVRVRGDMDAERLTDYLET
                     RLSPALARIPGVSKINLVGGSRREARITLNATELESRGLSALQIYEALRYNNLNVPAG
                     NASYRSRELSFTTDGSFSDLDDLRNLTVGFGDGSYIFLKDVAAITLEPEPRDIRVRSG
                     GDDYVMVDVMKRDEGDTLEISTRARAVLDEIGRESGGTIRYAVISDQSEITSRSLGTV
                     VETALTGLALTILVIVLVLHDIRATIIISLSIPLSILFAILGLYGTGRSLNLLSLSGM
                     TVAIGMIVDNSIVVLENTYQKFKKSGDRRLAAMTGASEVGGAVLASTTTSVCVFAPLL
                     FLTGIIGIIMNDLSLAIVFALAASALVSVIVVPWLASLILKREDQMRRPKALKAVEAA
                     IDRAFDRLERAYRRLLVAALGNKVFTLSSAVALLVASALLLTVLKISFLPPTDTGEFE
                     LHIETPAGYSLERTMAKVDELDALVASLVPEIEASVYYVGATGAISMTGTPNRAFGRI
                     RLVPSEARERTVQEILPLVQDALSRSLPDCDATVLNGGFDSLLALGTGGQGFQMEVYG
                     TDLDAVTETARLARDLLARDPDVFKTDLSVRMDAQQLYAELSQSYMGALGVTPYEAGV
                     TSRILFGGMDAGTLRVGGDDYPIRVISDVASERIDDDTLNRLSIRTQDGRLVSFAAFS
                     TVESRPALSRIDRKDRNFSVEVRGYLRSEDQAGVSSRMEAAMSGLSLPYGVKYRTTGT
                     SELIGESLGSLALALAIAVFLVYSVMVIQFERFMQPLIIMASIPFCLIGVVLGLYLFG
                     SSMSIIAMLAVIALGGTVVNNAIVMVDYINQLRRDYGLELREAILQGCANRLRPILMT
                     ALTTLFGVLPMALAHGNGSEVYAPLGQAIFGGLLSSTVITLVIIPVLYESLERGSRFI
                     QSAATAATELEINE"
     gene            complement(11399..12481)
                     /locus_tag="PLQ29_00140"
     CDS             complement(11399..12481)
                     /locus_tag="PLQ29_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPG85080.1"
                     /translation="MEANSRARRGPAIMTLRWLVPLALVAGATAFWLISSGQEPERPE
                     APPVPVRVMRPAYGDLVKTLKLNAHVESETMVTVVPLVSGILQELPVEVGQTVAAGQV
                     VARIDAQRFELQLKQAEAAYLSAKSSYERVGQLYRANAATQQSYEQAKGQYEAYSSQY
                     ELARIQLDYASVKSPIAGVVLVKHLSAGSIAAPERPLVTIGDLGDLVVRARVPERYYE
                     SFGSMRESMRIAIRREGGDEYPGVIRSVSPFVSAETKNFEVVVSIGGSPELLRPGMFV
                     SVVFELARWPGVHTLPYEALSGDGRLWWVDDGLARSLPFAPDAASDAAFAVPREWAAR
                     DVIVEGQYFASEGSPVTVVAPQEADR"
     gene            complement(12491..13102)
                     /locus_tag="PLQ29_00145"
     CDS             complement(12491..13102)
                     /locus_tag="PLQ29_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPG85081.1"
                     /translation="MLDTTRERILEAATAIIRERGPEGLTVEAAADRAGVSRKTVYNH
                     FDGKYALIDVAAAAWMDGLLADLGVIAADASLPFVTKLNAIVERAYAELKNQRRAGAA
                     GSMAASLDLPGFRADLQARLHDFIAGIVQDAIDEGLVRDDFDARRLTYVIINIVSGLA
                     VLAGIEDEPFTRVDILTDSLKALVGGILTPTGAEAMRGSPLLG"
     gene            13261..13755
                     /locus_tag="PLQ29_00150"
     CDS             13261..13755
                     /locus_tag="PLQ29_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HPG85082.1"
                     /translation="METRIEAVQLSWTDGANIIVGQSHFIKTVEDIAEIMATSVPGAR
                     YGLAFNEASGPCLVRTAGNDEGLVRDATACALATGAGHTFYLVLGAGAYPINVLDRVK
                     ACQEVCRIFCATANPVQAVVARTAQGSGLLGVIDGSSPAGVEGPADKAERIGMLRAFG
                     YKFA"
     gene            13793..14677
                     /locus_tag="PLQ29_00155"
     CDS             13793..14677
                     /locus_tag="PLQ29_00155"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPG85083.1"
                     /translation="MHVSGTTIAFIGTGVMGASMAGHLLRAGATVRVYNRTKSKADPL
                     LSAGALWAESPAEAVKGASVVFTMLGYPSDVEQVYLGSGGIIAASAPGAVLVDFTTSD
                     PALAARISAAAAASGRSAIDAPVSGGDTGARNATLSIMVGGDEATFSAVKPFFDVVGK
                     TAVLQGGPGAGQHTKMANQISVAASLIGAVESMTYARNAGLDPRRVLDSIGGGAAQSW
                     QLVGMAPRMLDGDFKPGFYSKHFLKDLRIALDSAKAMGLELPLLALAESLFERMQEKG
                     FGDLGTQALYRLYQGGGI"
     gene            14677..15762
                     /locus_tag="PLQ29_00160"
     CDS             14677..15762
                     /locus_tag="PLQ29_00160"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HPG85084.1"
                     /translation="MAADSRDEARPPYARDLTDPPPSVVRDGEFVYGCFNAPPALVNM
                     LDVRRPYHYPIPRWTKDARCKEWVAFQFGDGRRFFLAALYEAKSFGLIQFVSWDRDGG
                     RTIESRRVIPLRRFGLGPVLDGRRVSYRDRHGALGFSFDLSGGSITVEASRARSSRGP
                     AYAGSFTFAYNSRQSAPSSVCLPLGLNRAMYSTKVLMPMRGWFETGGEHVEFDSPECM
                     GVMDDHKGYYPYGMRYDWVTGFGLAGKGRRVGFNLTDNQVRDQERYNENVLWVNSRVY
                     PLPPIKVTRPNGVDNPWHVQDTEGLVDLVFVPERKNDLRINLLFASVDYHGPFGTFSG
                     TLRSPDGSVKVDAEGLLGMGEQKYLRA"
     gene            15773..16534
                     /locus_tag="PLQ29_00165"
     CDS             15773..16534
                     /locus_tag="PLQ29_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPG85085.1"
                     /translation="MATRGSFRTVDVARAAGTHENTVRLYVAWGFLAEPERAANGYRR
                     WTAEHIDQMLFARLAMHGMWPGRPIRESALALVRRAATGDLTGAVADARIHAALVDAE
                     LARAESAAAFLERWAAGAAPAAGDEGRLGPREAAAAVGATPGQIRNWERNRLVASVKD
                     PRTGHRVYGPDQIGRLRVVRSLLLAGYSVMAILRMATELDRGRSTGLKDVLNTPRPGE
                     EALTAFDAWLDSLAEQKARAERLIAMLAARIEANR"
     gene            complement(16588..17349)
                     /locus_tag="PLQ29_00170"
     CDS             complement(16588..17349)
                     /locus_tag="PLQ29_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG85086.1"
                     /translation="MRNPGTLIVGRALRRLVSNPAPLLSGLGMSAFFLFVYDAAIGGI
                     GFLPEFGGAGYMAFLLPMGVISLLFGSSAGSAQALSRDIGSGYFMRLALAPVPRSAFV
                     LAAILADAAGIFVSSLIVLGLGALLGAPLLGGAVGVFGTAALATLFGAGISAASAAAV
                     MRTGKVELAGTIGSAVFMLLFLAPTFVPRELMGARWLQVVSAWNPLSYLMEAMRVMVS
                     GLGEPSSVPIAFAVAAVAGLGGTAAACLSVRKVLN"
     gene            complement(17342..>18075)
                     /locus_tag="PLQ29_00175"
     CDS             complement(17342..>18075)
                     /locus_tag="PLQ29_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG85087.1"
                     /translation="GPRALAGVDLDVPEACRFALLGANGAGKSTLVRILCTLSRQDSG
                     RVAIAGRPISGGSQAIRALIGVALQDTQLDPAESARRQLAFQGRLYGFDAARAAGRAE
                     ELLARFGLELVADRKAAELSGGNKRRLHVALALVHRPRVLFLDEPSVGMDPESRAAFW
                     AEMRRLNAEEGACVFFTTQYLEEAEKHGDELAVLDSGLVAYRGTVDGFAAAHAGPGGD
                     LEAGYLNFIESRRRGALSEKEAVGA"
BASE COUNT         2909 a         6282 c         6059 g         2825 t
ORIGIN      
        1 ggaagcgcct gatggcctac ctggccgacc atcccgtggc cgccctgccg ccgggcgatc
       61 cggcctggtt cgaggcccac atcatgcatc cggccagcgc ggcgaccggc gactacgagg
      121 tgttcatagg cgccgaggcg gagcggccgc tcgacgcccc ggtcgagctc gtggtcaagt
      181 cgttgccagc cagagactac gccgtgctct cgatgcgctg cgcggagatg gagccgggct
      241 gggaggatcg cgtctcggcc gaggtcgtcg gccgctacgg acgggcgatc gagcgacgat
      301 gggggctcca gcgctacgat tcccgcttct cgagcctcga ggatccgggc tcgatcatag
      361 agatgtggac gccactagcg cccgagaccc ccgacggaga ggcgcattga ctgacgccga
      421 caggcgcctc gcgtcggtcg gccgagccct ggacctcgtc gaggagcggc tacgcgacga
      481 cctcgacgtg gccgcgctgg cgagcgccgc ctgctactcg atcttccact tcgtgcgggt
      541 cttcaccgag ctgaccggcc acggacccta cgactacctg atgcgcaggc gcgtcgcgga
      601 agcggcgcga gagctcgtct ctggcgaccg gtccatcctc gagctggcac tcgactaccg
      661 cttcgagtcg ccggacggct tctcccgagc cttcaggcgc tgctatggcc tagcgccgag
      721 cgaggcgcgt aaggcaggcg ccttccccga cggctcggcc aggacgccga ttacccggga
      781 gtacgtacac gcatccatga acgccgggct gacgatacgc gccgaacggc taagcgacgt
      841 cctggtctca ggccgctccc tggcgccggg cgaggccgcc ggctccgagg cccggctcat
      901 gatactcggc accgggcgcg acgcattcgc cggccgggcc gccgacgagg tgccagacta
      961 cccgacactc ggcacgcgcg taccgggagg cgactgtatc gtcgcttctg gccagccgcc
     1021 actcgacctt ggcctggccc gcgaacggct gtaccgcacg gccattcccg tcggctcggc
     1081 gcgggtgcga gccgacttcg agcttatccg cctggatagc ggaggcggcc cgatcgctat
     1141 actcgtaccg gtcttgctct ccgagccgct cggcgtcaag atggggcggg gggatagatg
     1201 gaacgacaga ccatacgcgc cggagagtcg gatccgcggg ccgaggctcc ggtgacgata
     1261 gccagcctgg ccctgctcgg ctgcggcacc gtcggcggcg ccttgatcga cctgatagcc
     1321 gaacgagccg atgcgctcgc cgcgcgcggc gtcgagccgc gcgtcgtcgc gatagccaac
     1381 tcgaggcgca tggtcgtcgc cccggacggg atcgacctgg cccgctggcg cgaggccctg
     1441 gcggccgcgt ccgagccgac agacccctcc gcgctcatgg cgctcggccc gtccctgccc
     1501 ggaccggtac tcgtcgactg caccgcgagc gacgagacgc cgcgcgccta tccgggcttc
     1561 ttcgcggcgg gcttcgacgt ggtgacggca aataagcgcg gcaacgcggg gccgctcgag
     1621 cactatcgga acctggcgcg cgaggcgcgg cggcgtggcc ggcagtacct gtacgaggcg
     1681 accgtcggcg ccggcctccc cgtcgtcgac aacctgcgca ggctgttcat ggccggcgac
     1741 tcgctgctcg acttcacggg cgtgctgtca ggctcgctat ccttcctgtt cggccgcctc
     1801 gaggacggcc agctcttctc ggccgccgtt cgcgaggcga tggcccgcgg ctttaccgag
     1861 cccgacccgc gcgacgacct gtcgggcgcc gacgtggccc gcaagacgct gctcctggcc
     1921 cgcgaggccg gcatggcgct cgagcccgag gacgtcgagc gcgccggcct gatcccgccc
     1981 gagtacctcg cgatggcgcg ggacgagttc gtcgcccgac tgcccgagct cgacgaaggc
     2041 gtcgccgcgc tgacccgatc ggcctccggg cgcggcgccg tcctgcgcta cgccgggtcg
     2101 atagccggcg ggcgcgcctc ggtcggcgtc gtcgaggctg ggcccggcca cccgctacgc
     2161 tccgtccgcg acggggagaa cgcgctcgcg ttccataccc gctactacga cccggtgccc
     2221 ctcgtcgtgc gcggctatgg cgcgggcgcc ctcgtcacgg ccgcgggcgt cctcggagac
     2281 gtgctgcgcg cggccgggca ctgacggcca ggcaagtccg ttacccgtca agcgagggct
     2341 gctacgccct cgagtccgac gacgctgaag tccgagccga cctgggcctg gtctcagcgc
     2401 agcgcggcct tgagcgcctc gaccttgtcg gtccgctccc agggaaagcc gtcgcggccg
     2461 aagtggccgt aagcggccgt cctccggtag atcggcgcct tgaggccaag gccgctcacg
     2521 atgccggcgg gcgacaggtc gaaggtcttc cgcaccgcgg cgacgatggc ctcctcgtcg
     2581 gccttggccg tgccgaaggt ctcgagcatc accgacaccg ggaaaggcac gccgatggcg
     2641 taggcgagct gcacctcgca gcgcgaggcg aggcccgcgg cgaccacgtt tttggccacg
     2701 taacgggcca tgtacgcggc cgagcgatcc accttggacg ggtccttgcc ggagaacgcg
     2761 ccgcctccgt ggcggcccat gccgccgtag gtgtcgacga tgatcttgcg gccggtcagg
     2821 ccggagtcgc ccatcgggcc gccgacgacg aagcggcccg tcgggttcac gtagaacttc
     2881 gtcttgtcgt cgagcagacc cgtcgggccg agcaccggcc gcacgatacg ctcgatcacg
     2941 gcctcgcgga tcgtctcgta ggacacgtcc gggtcgtgct ggtgcgaaat gacgatggct
     3001 tccgctctga tcggcttgtg gccgtcgtat tcgagcgtca cctggctctt ggcgtccggc
     3061 cggagccacg gtatcgcctt ggacttccgc aacgcggtcg cctcgagcag cagcttgtgg
     3121 gcgtacatga taggcgccgg catcagctcc ggcgtctcgt cgcaggcgaa gccgaacatc
     3181 atgccctggt cgcccgctcc ctgctggccc tcgtattcct tgaggccgtg gccgaccacg
     3241 ccctggttga tatcctggct ctgcgagtgg atcgtgttga ggatggccat cgactcgtag
     3301 tcgatgccgt agtcgggatc ggtgtacccg atctccttga ccacgtcgcg gacgagggtc
     3361 tggatgtcca cgtacgtgtc ggtggttatc tcgccgccga ccagcaccat gccggtcgtc
     3421 gcgaagcact cgcaggccac atggctctcc gagtcgtcgc ggaggcaggc gtcgagcacc
     3481 gcgtccgaga cctggtcgca tagcttgtcc gggtggccct cgcccacgga ctccgaggta
     3541 aagagcttgc gcttgtctga catggggaaa tctcctcttt tagctgtatc ctcgccgcga
     3601 tggttttcgc tacgagccgc ccgcaatacg gatcaaatcg gcggagcgga cgatcccgcc
     3661 cgggactcgt ccgccgggta aatatcgact atttggccgt cttcggcaag gccccgagct
     3721 acgtcggggc ccgcgcccca cgggcgcgcc gcttaccagc cgtcgcggcc ggcctgagcg
     3781 gcgctcgggc tcggcgcgtt ttacaaagcc ggcgaacggc cctatactga tgccattcca
     3841 tccccaagcg gagcggttcg atgcagcaca acgacaagta catcctcgcg ctggaccagg
     3901 gcaccacgag ctctcgcgcc atcgtcttca accagaccgg cgtcgtcata ggcatgaaac
     3961 agatagcctt caaccagatc tacccgaagc ccggctgggt ggagcacgac cccttcgaca
     4021 tctggcagag ccagtacgac gcggcggtcg gcgccatcga cgaggcgcag atcacgccgt
     4081 cgcagatcgc cgcgatcggt atcaccaacc agcgagagac tacgctgatc tgggaccgta
     4141 agaccggtaa gcccgtgcac aacgccatcg tctggcaatg ccggcgctcg tccaggatct
     4201 gccaggagct caaggccctc ggcttcgagc ccgagatcaa ggcgcgcacc gggctgatcc
     4261 tcgacccgta cttctccggg accaagctcg tctggctgtt cgaccagata ccgggcctac
     4321 gcgaccgggc cgagcgcggc gagctggcgt tcggcaccgt cgactcctgg ctgatctaca
     4381 acctgaccgg caggcacgcg accgacgcca cgaacgccag ccgcaccatg ctgttcaaca
     4441 tccgcgaggg ccgttgggac caggagatcc taatgatgct caagatcccc gaggccatcc
     4501 tgcccgaggt gctgccgagc tcggccgact tcggctacac gcgcaaggat ctcttcggct
     4561 gcgagatacc ggtctgcggc tgcgccggcg accagcaggc cgcgctattc gggcacgcct
     4621 gtttccaggt cggcgccagc aagaacacct acggcaccgg ttgcttcgcg ctgatgaaca
     4681 ccggctccga gccggtcgag tcgaggcaca acctgctcac gacgatagcc tgggacctgg
     4741 gcaagggcta tacctacgcg ctcgagggtt ccgtgttcat aggcggggcg gtgatccaat
     4801 ggctacgcga cgagatgcgc ctcctatccg actccgccga gagcgagcgg ctggcgcggc
     4861 tggtcaagga caccggcggc gtcgtcctcg tgccggcctt cgtcggcatg ggcgcgccgt
     4921 actgggacgc cgaggcccgc ggcgccgcgc tcggcgtcac ccgcggcacg accaaggccc
     4981 acttcgtgcg ggcagccctc gagtcggtag cctaccagtc gcgcgacctg ctcgacgcga
     5041 tggagcgcga cgctggcgcc tcgataccgt tcatcaaggc ggacggcggc gccagcgcca
     5101 acagcttcct catgcagttc caggccgacg tgctcgacaa gccggtcatc ctgccggagg
     5161 tgggcgaggt taccgcgctc ggcgccgcct acctggcagg cctcagggta ggctactggt
     5221 ccgggctgga cgagctggag cgcaactggc gccgcaagcg cgacttcatg cccgagatgg
     5281 cagccgagac gcgcgatcgc ctgctcgagc gctggtccaa ggccgtcgag gtgacgcgcc
     5341 tcttcaagta agccgcgtcc cggaggccgt cggacaccgc cactcggtca cggcgccgtc
     5401 ggcgcttcca tcggagcggt cgccggcgcc gcaggcgacg gcgagcccgc gagcgcatcg
     5461 agcgacacaa gggcgccgcg gtactcggcc agcgtgccgt aggcctcggc cagcgagccg
     5521 agcgcctcga tcatgccggt caggtactcc gactccgagc cgcctccggc ccgcagggcc
     5581 gcccgcttct gcgccaggtc ggccgcccag ccctcctgcc tgagcgccgc gtactcgagc
     5641 cgggcgcggc ccagctccat gcccagccag gtatcgcgca caaaggaccg gatggcccgc
     5701 tcggcgtcgg cccgctgcgc ggcggcttcg gccagcaagg cccgagcctt ggcggcctcg
     5761 ccctccttga ccgcgttccc aaagatattg cccgaggtgc ccacgctgaa ggtcaactga
     5821 tagtcgtcct gcccgaacca gtccttctcg acgaagggca ggcgcgagcc gccgtacgac
     5881 agctccacgc gcaggccgat gtctggccgg cccttcgcct cggcctcggc caggtcgcgc
     5941 aggccgcgcc tggccacgag ctgcgcctcg agcaaggcca ggtcgtagct gttcccgagg
     6001 gccagcgcgc cagcttccgc ctcggtccag cgcggcgagc cggcgatcgg cggagtcatc
     6061 tccaggtcat ccttcgatag agcgtcgagg ccagccatcg acgcgagttc ggcgagcaat
     6121 ttgtccctac gctcgtcgag acgcactctg gctatgtcaa tctccttgag cttgatgcgc
     6181 gcgccggcca agtcggcccg ggtcatgaag ccggcctcag cgctcgcctc ggccagttcg
     6241 acgaggcgtc gcccgatcgt agcctgtagg ccgagcatct cgtcggccgc ccgcaggcag
     6301 gccagcgcgt cccagtcgcc ccgcatcctg gcggccagct cgcgctcggc cttgcgccgc
     6361 tgcaggccag cggcgtccag gcccgcccgg gccaggtcca ccccgatggc tattttgccc
     6421 caggtgtaca gcggcacctc ccctatcagc gcaaaatcat agttgtagct ctccatcccc
     6481 ttgtagatga tcacgtcctc gggcggtatc gagacgccct ggaacgcgcc gagctggccg
     6541 gcggtcagcg cgataggccc catggggttt ccgatatacg tgccggaggt ctccgccctg
     6601 agggtcggca ggcgcctcgc cctcgcgccc gccaggtccg ccgcggccgc cgcctcggcc
     6661 tcggcgagcg cccgcagccc gggatttcgc ccggcggcca acgccagtag ctcgtcgagc
     6721 gaccagggcg taaggccgtc accggccacg gcccccgtgg cgggcagatc gacgaccggc
     6781 tcgaacggcg cagagaagtc tatcgccgcc cggtacgccg agaccgccgc ctcgtagccg
     6841 gtcgcgaacg gatcggccgg ggacgtcgcg gacgcgggcg cctcgaggcc gctctcctgc
     6901 gacacggatt gcgccgtcgc cgtcgacgcg gcccaggcca atagcccgat gacggatacc
     6961 atcacccgct tcgtcagggt catggcgtcg ttacccggag cgtcacgttg cgctgcgtgt
     7021 ccggcttaag agtatccagc tttcctgaga caagcgtcgc gccggtcgcc gtcatcgtca
     7081 catcgacctc gccgaccttc caatccgtac tgcctgaccc atcgcctatc tcccagacaa
     7141 tcgtacccag gtcgaaacgg ccgttctcag tgctcgcggt cgtatcgacc ggagcgctgt
     7201 cggccgagta ctctcgatcc ttaacggtca actcgacggt gacgccgttg attccggtaa
     7261 taccgtcggt gtcatcaacg acacgtccgg ccaccgtcgc gctgaagcgc cagcgctaca
     7321 tcgtcacggt cggcgctgcg ccgaggacgg cctcgccctc gtccgcgtcg tactcgagcg
     7381 tggcctggac gtaggcgtcc tcatagtcgc cctgggccgc cgtcttgccg tcgcggtcga
     7441 ggtcggcgcc aagcatgacg gcggtgccgt tcgtgaacga ggtggagccc tcatcgtcgt
     7501 tgcatcggta gttcgtcaat acgtaggtga tcctgaccgg cgcgttatta tgggtcgcgt
     7561 gcgtcgcgcg gtcgggcctc atcgggaagc gcacggtcgc gacccaggcg ccgtcggcgt
     7621 cggagctcgt cgtcgcggtc ggagccgtgg gatagacgat gtccgtgaag ccgccggagg
     7681 cgtcataggc ccaggactcg gcaacgtaga agttgaccgt cacaccgccg agtcccttgt
     7741 cgtccttcca gtccagcacc gtccccgcga gccgcccctc gttccagagg tcctcgatct
     7801 tgatgtagtc gagccggttg gtcacgtcgg agacgacgta gagcggggtc gggttcttcc
     7861 agaggccgta atcggggtga tagatcagga aaaacacctc gtcgcggtcg gcggtcttgc
     7921 cgtatttcgg gaacagggac tcccagatga agccggtgaa gtcgtagccg ccgtcggcgt
     7981 cggtaacggt gctctggaag tacccgcgct cggttcgcgc cgagccgtcg ggcagggtgg
     8041 actcgtcgcc gtcgacgaag gaagcgtagt cggcgtcgcg ggccttgtcg tcggtataga
     8101 ggaatagctt ggcgtcggcc acgccggtcg tcgtcccgtc gtcccagtcc tccaggtcga
     8161 tgatcgaacc ctggatcgac gagcggaaaa ccgccgagca cgacgcggcc aggccggccg
     8221 ccaccgcgat gatgatgagc gccgcgatcc ggcgcgttgt cgctagatta ctcattgatc
     8281 tcgagctccg tcgcggccgt ggccgccgat tggatgaagc gggagccccg ttcgaggctc
     8341 tcgtatagta cgggtatgat gacgagggtg atgacggtcg aagacaggag cccgccgaag
     8401 atggcctggc cgagcggcgc gtagacctcg gagccgttgc cgtgggccag ggccatcggc
     8461 agcacgccga acagcgtggt cagggcggtc atcaggatgg gcctgagacg gttggcgcag
     8521 ccctgcagaa tagcttcccg cagctccagg ccgtagtccc gcctgagctg gttgatgtag
     8581 tcgaccatga cgatggcgtt gttcacgacg gtgcctccga gcgctatgac cgcgagcatc
     8641 gcgatgatcg acatggacga gccgaacagg tagaggccca gcacgacccc gatcaggcag
     8701 aacggtatcg aggccatgat gatgagcggc tgcatgaagc gctcgaactg gatgaccatg
     8761 accgagtaga ccaggaacac cgcgatggcc agagccagcg caaggctgcc gagcgactcg
     8821 cctatgagct cgctggtgcc ggtcgtccgg tacttgacgc cgtagggcag cgacaggccg
     8881 gacatcgcgg cctccatcct ggagctgacg ccggcctggt cctcgctgcg caggtagccg
     8941 cgcacctcga cggagaaatt acggtccttg cggtctatcc tggacagggc cggccgagac
     9001 tcgacggtgg agaacgcggc gaagctgacg aggcggccgt cctgggtgcg tatcgacagg
     9061 cggttcagcg tgtcgtcgtc gatgcgctcg gaagccacgt cggagatcac gcggatcggg
     9121 tagtcgtcgc cgccgacgcg cagcgtgccg gcgtccatgc ccccgaacag gatgcggctc
     9181 gtgacgccgg cctcgtacgg cgtcacgccg agcgcgccca tgtaggactg gctcagctcg
     9241 gcgtacagct gctgggcgtc catgcgcacc gacaggtccg tcttgaacac gtcggggtcg
     9301 cgggccagga ggtcgcgggc cagcctggcc gtctcggtga cggcgtccag gtcggtgccg
     9361 tacacctcca tctgaaagcc ctggccgccg gtgcccaggg cgagtagcga gtcgaagcca
     9421 ccgttcagca ccgtcgcgtc gcagtcgggc aggctcctcg acaaggcgtc ctgcaccagg
     9481 gggaggattt cctggacggt acgctccctg gcctcgctcg gcacgagccg gatgcgtccg
     9541 aaggcgcggt tcggcgtgcc ggtcatcgag atggccccgg tagcgccgac gtaatagacc
     9601 gacgcctcga tctccggcac gagggacgcg accagcgcat ccagctcgtc gaccttggcc
     9661 atcgtgcgct cgagcgaata gcccgccggc gtctcgatat ggagctcgaa ctcgccggta
     9721 tcggtcggcg gaaggaagct gatcttgagc acggtcagga gcagggcgct ggcgacgagc
     9781 agcgcgacgg ccgaggacag ggtgaacacc ttgttgccca gcgccgcgac gagcagccgc
     9841 cgatatgcgc gctcgagccg gtcgaaggcc cggtcgatcg ccgcctctac ggccttgagg
     9901 gccttgggcc ggcgcatctg atcctcgcgc ttcaagatga gcgacgccag ccacggcacg
     9961 acgatgaccg ataccagagc cgaggccgcc agagcgaaga cgatggccag ggacaggtcg
    10021 ttcatgatga tgccgatgat gccggtcagg aacaggagcg gcgcgaacac gcagacgctc
    10081 gtcgtcgtcg acgccaacac ggcgccgccg acctcgctgg cgccggtcat cgccgccagc
    10141 cggcgatcgc ccgacttctt gaacttctgg taggtattct ccaggacgac gatggagttg
    10201 tcgactatca tgccgatcgc gacggtcatg cccgacagcg acagcaggtt gagcgagcgc
    10261 ccggtgccgt acaggccgag gatcgcgaac aggatcgaca ggggaatgga caggctgatg
    10321 atgatcgtcg cgcgtatatc gtgcagcacg agcacgatca cgaggatggt caaggccagg
    10381 ccggtcagag ccgtctcgac gacggtgccg agcgagcgcg aggtgatctc gctctggtcg
    10441 ctgatcaccg cgtaccgtat tgtgccgcca gactcgcgcc cgatctcgtc gagcaccgcc
    10501 ctggcccgcg tggagatctc cagcgtatcg ccctcgtcgc gcttcatcac gtcgaccatc
    10561 acgtagtcgt cgccgccgga gcgtacgcgg atatcgcggg gctcaggctc cagggtgatc
    10621 gccgcgacgt ccttgaggaa gatgtacgac ccgtccccga agccgaccgt caggttccgg
    10681 aggtcgtcca ggtcggagaa cgatccgtcg gtggtgaagg acagctcgcg ggaccggtag
    10741 ctcgcgttgc cggcgggcac gttcaggttg ttgtagcgca acgcttcgta gatctgcagg
    10801 gccgacaggc cgcgcgactc gagctcggtc gcgttcagcg tgatcctcgc ctcgcgcctg
    10861 gaacctccga cgaggttaat cttggacact ccggggatgc gcgccagggc cgggctcagc
    10921 ctcgtctcca ggtagtcggt caggcgctcg gcgtccatgt ccccccgcac ccggaccgag
    10981 aagaccggca ggaagccgct cgcctcggcg atgaaaacca cgggcgcccc ctcgatgccg
    11041 ctcggcaggt cgttcgcgat gccgttcagt agctcgcgga tctgcggcag tttggaaccg
    11101 acatcgacat cggcgcggaa ctcgagggat accgtcgagt acgagtcagc gcttgacgac
    11161 gacatttcgg tgacgccggc caggctggat agctggttct cgatcttcct ggtgatgtcc
    11221 cgctccacgt ccttggcgcc ggcgccggga tagaccgtca gcacggtcgc gatcggcagg
    11281 ccgaccggcg gtatcatctc ggagttcagc gacgagccgg cgatcaggcc gaataccacc
    11341 aggccagcca gcaggatcgt gacgacggcg gggtgcttga cggagaaatc ggacagtatc
    11401 atcggtcggc ttcctgtggc gccaccacgg tcaccggcga gccctcgctc gcgaagtact
    11461 ggccctcgac gatcacgtcg cgagccgccc attcccgggg caccgcgaac gctgcgtccg
    11521 acgccgcgtc cggcgcgaag ggcaggctcc tggccaggcc gtcgtcaacc caccagagcc
    11581 gtccgtcgcc ggacagcgcc tcgtagggca gggtatggac gccaggccac cgggccagct
    11641 cgaacacgac cgatacgaac atgcccggcc ggagtagctc tggcgagccg cctatcgaca
    11701 cgacgacctc gaagttcttc gtctcggcgg acacgaaggg gctgacgctg cggatgacgc
    11761 cggggtactc gtcgccgccc tcgcgccgta tcgctatgcg catggactcg cgcatggagc
    11821 cgaaggactc gtagtagcgc tccggcacgc gggcccgcac gaccaggtcg cccaggtcgc
    11881 ctatcgtcac gagagggcgc tcgggcgccg ctatgctgcc ggcgctcagg tgcttgacga
    11941 gcaccacgcc ggctatcggg ctcttgacgc tcgcgtagtc gagctggatg cgggccagct
    12001 cgtactggga cgagtaggcc tcgtactggc ccttggcctg ctcgtagctc tgctgggtcg
    12061 cggcgttggc gcggtagagc tgaccgacgc gctcgtacga cgacttggcc gacaggtagg
    12121 ccgcctcggc ctgcttgagc tgtagctcga agcgttgcgc gtctatcctg gccacgacct
    12181 gcccggcggc caccgtctgc ccgacctcga ccggcagctc ctgcaggatg cccgagacga
    12241 gcgggaccac ggtgaccatc gtctcggact cgacgtgggc attcagcttg agcgtcttga
    12301 ccaggtcgcc gtaggcggga cgcatgacgc ggaccggtac cggcggcgcc tcgggccgct
    12361 ccggctcctg gccggacgat atgagccaga acgccgtagc cccggcgacg agcgccaatg
    12421 gcacgagcca gcggagcgtc atgatggccg gaccgcggcg cgcgcggcta ttcgcttcca
    12481 tcgtcgcccc ctatccgagc agcggcgagc cgcgcatggc ctcggctccg gtaggcgtca
    12541 ggataccgcc gaccagagcc ttaagcgaat cggtcaggat gtcgacgcgc gtgaacggct
    12601 cgtcctcgat gccggctagt accgccaggc ccgagacgat gttgatgatc acgtaggtca
    12661 gccggcgcgc gtcgaagtcg tctcgcacga gcccctcgtc gatggcgtcc tgcacgatgc
    12721 ccgcgatgaa gtcgtggagc cgagcctgga ggtcggccct gaaccccggc aggtccaggc
    12781 tggcggccat cgagccggcc gctccggcgc gtcgctgatt cttgagttcg gcgtaggcgc
    12841 gctcgacgat ggcgttgagc ttggtgacga agggtagcga cgcgtctgcg gctataaccc
    12901 ccagatcggc gagcaggccg tccatccacg cggccgcggc cacgtctatc agcgcgtact
    12961 tgccatcgaa atggttgtat acggtcttac ggcttacgcc ggcgcggtcg gctgcagcct
    13021 cgaccgtcag cccctcaggg ccgcgctcgc gtatgatcgc cgtcgccgcc tccaggatcc
    13081 tctctctcgt cgtatcgagc attgtcatag tccttccaca tattacacag ttgctgtgta
    13141 atatgttacc tcctggtaca catttcaata gcttttgtgt aaaatcggtg aaaagctcgc
    13201 gcaaacccga gcttgcggct aaagcgctaa gcggatagac tggctggccg gaggacgcat
    13261 atggagacga ggatcgaggc cgtccaactc tcgtggacgg acggcgctaa catcatcgtt
    13321 ggtcagtcgc acttcatcaa gacggtcgaa gatattgctg aaatcatggc cacctcggtc
    13381 ccgggggcca ggtacgggct agccttcaat gaggcctcag ggccttgcct ggtcaggacc
    13441 gcgggtaacg acgaggggct cgtcagggac gcgacggcct gcgcgctcgc gaccggcgcg
    13501 ggccacacct tctacctggt gctcggtgcc ggggcctatc cgatcaacgt cctggaccgg
    13561 gtcaaagctt gccaggaggt ctgccgcata ttctgcgcca cggccaatcc cgtccaggcc
    13621 gtcgttgcca gaacggccca gggctcagga ttgcttggcg tcatcgacgg ctcgtcgcca
    13681 gccggtgtcg agggccccgc ggacaaggcc gagcgcatcg gcatgctcag agccttcggc
    13741 tataaattcg cgtaaggccg cggtcgcgcc gcgcgttatc tcggagggat tcatgcacgt
    13801 ttcaggcacc accatagcgt tcatcggcac cggcgtcatg ggcgccagca tggccggcca
    13861 cctgctcagg gccggcgcga cggtacgcgt ctataaccgg accaagtcca aggccgaccc
    13921 gctcttgtcg gccggagcgc tctgggccga gtcgccggcc gaggccgtca agggcgcgtc
    13981 ggtcgtcttc acgatgctcg gctatccgtc ggacgtcgag caggtctacc tcggctcggg
    14041 cggcatcata gccgcctcgg cgcccggcgc cgtcctcgtc gacttcacga cgagcgaccc
    14101 cgctctcgcg gcgcgcattt cggccgccgc ggcggcttcc ggcaggtccg cgatcgatgc
    14161 cccggtatcc ggcggcgaca cgggcgcgcg caacgccacg ctatcgatca tggtcggcgg
    14221 cgacgaggct acgttctcgg cggtcaagcc gttcttcgac gtggtcggca agaccgccgt
    14281 gctacagggc gggcccggcg ccggccagca taccaagatg gccaaccaga tatcggtggc
    14341 agcgtcgctc atcggcgctg tcgaatcgat gacctacgcc cggaacgcgg gcctcgaccc
    14401 gcgccgggtg cttgacagca tcggcggcgg ggcggcccag agctggcagc tcgtcggcat
    14461 ggcgccgcgc atgctcgacg gcgacttcaa gcccggattc tattcgaagc acttccttaa
    14521 ggacctgcgg atcgccctcg actccgccaa agcgatgggc ctcgagctgc cgctcctggc
    14581 gctcgccgag agcctcttcg agcgcatgca ggagaagggc ttcggcgact taggcacgca
    14641 ggccctgtac agactctacc agggcggcgg catctgatgg ccgcggactc ccgcgacgag
    14701 gctcggccgc cctacgccag ggatctgacc gacccgcccc cgagcgtggt ccgcgacgga
    14761 gaattcgtct acggctgctt caacgcgccg ccggccctcg tcaacatgct cgacgtgcgg
    14821 aggccgtacc actaccccat cccgcgctgg accaaggacg cccgctgcaa ggagtgggtc
    14881 gcgttccagt tcggcgatgg gcgccgcttc ttcctcgcgg cgctgtacga ggccaagagc
    14941 ttcggtctga tacaattcgt atcgtgggac cgggacggcg gcaggaccat cgagagccgg
    15001 agagtcattc cgctcaggcg cttcggcctc ggccccgtgc tcgatggcag gcgagtctcg
    15061 taccgtgacc gccacggcgc gctcggcttc tcgttcgacc tgtccggcgg gtcgatcacg
    15121 gtcgaggcca gccgcgcccg gtcctctcgg ggtccggcct acgcgggtag ctttaccttc
    15181 gcgtacaact caaggcagtc ggccccgtcg tcggtctgcc tgccgctcgg cctgaaccgg
    15241 gccatgtact cgaccaaggt actcatgccg atgcgcggct ggttcgagac cggcggcgag
    15301 catgtcgagt tcgactcgcc cgaatgtatg ggcgtcatgg acgaccataa gggctactac
    15361 ccctacggta tgcgctatga ctgggttacc ggcttcgggc tggcgggcaa gggccggagg
    15421 gtcggcttca acctgaccga caaccaggtc cgcgaccagg agcgctacaa cgagaacgtg
    15481 ctctgggtca acagccgcgt ctacccgctg ccgcccatca aggtgaccag gccgaacggc
    15541 gtcgacaacc cctggcacgt gcaggacacc gagggcctcg tcgacctggt cttcgtaccc
    15601 gagcgcaaga acgacctgcg gatcaacctg ctcttcgcct cggtcgacta ccacgggccg
    15661 ttcggtacct tctcgggcac gctgcgctcg cccgacggct cggtcaaggt cgacgccgag
    15721 ggcctcctcg gcatgggcga gcagaagtac cttcgggcgt aacggaaggc gcatggcgac
    15781 gagaggctcg ttccgtacgg tggacgtcgc cagggcggcg ggaacgcacg agaatacggt
    15841 caggctctac gtcgcctggg gttttctcgc ggagccagag cgcgccgcca acggctacag
    15901 gcgctggacg gccgagcata tcgaccagat gctattcgca cggctagcga tgcacggcat
    15961 gtggcccgga aggcccatac gagaatccgc gctggcgctc gttcgtcgcg ccgcgacggg
    16021 agacctgacc ggggccgtag ccgacgcccg catccacgcg gccctcgtgg acgccgagct
    16081 agcgcgcgcc gagagcgcgg ccgccttcct ggagcgctgg gcggcgggag cggcccccgc
    16141 cgcgggcgac gaggggcggc tcggcccccg agaggccgcg gcggccgtag gcgcgacgcc
    16201 gggccagatc cggaactggg agcgcaaccg cctcgtagcg tcggtcaagg acccgcgcac
    16261 cggacatcgg gtctacgggc cggaccagat aggccggctg cgggtggttc gatcccttct
    16321 gctcgccggc tacagcgtca tggcgatact gcgcatggcg acggaactcg accggggccg
    16381 ctcgaccgga ctcaaggacg tcctcaacac gccgcgccca ggcgaggaag cgctgaccgc
    16441 cttcgacgct tggctcgact cgctggccga gcagaaggcg cgggcggagc gcctcatagc
    16501 gatgctcgcg gccaggattg aagccaaccg ttagcgcgtt cgcggcctga accacggcgc
    16561 ccatcctccg actcgtcgcc tccagcccta gttcagcacc ttcctgacgg agaggcacgc
    16621 ggccgccgtg ccgccgagcc cggccaccgc cgcgacggcg aacgcgatag gtaccgaact
    16681 cggctctccc aggcccgata ccatgacccg catcgcctcc atcaggtagc tgagcgggtt
    16741 ccacgccgag acgacctgga gccagcgagc gcccatcagc tcccgcggca cgaaggtcgg
    16801 ggcgaggaac agcagcatga agacggccga gcctatcgtc cccgccagct ccaccttgcc
    16861 cgtccgcatc accgcggcag ccgaggccgc ggatatcccg gcgccgaaca gcgtggctag
    16921 agccgcggtt ccgaacacgc cgacggcccc tcccaggagc ggcgccccga gcagcgcgcc
    16981 caggcccagc acgataagcg acgacacgaa gatgcccgcc gcatcggcca ggattgccgc
    17041 gagcacgaag gccgacctcg gtacgggcgc cagcgccagc cgcataaaat agccggagcc
    17101 gatatcccgg ctcagagcct gggccgagcc cgccgacgac ccgaacaaga gcgatatgac
    17161 gcccatgggt agcaggaagg ccatataccc cgcaccgccg aattcaggta ggaagccgat
    17221 gccgcctatc gccgcgtcgt agacgaatag gaagaacgcg ctcatgccca ggccggacag
    17281 tagcggcgcc ggattggaaa cgagccgcct gagcgcccgt ccgactataa gcgttccggg
    17341 attacgcacc gacggcctcc ttctccgata acgcgcctcg gcgccgcgac tcgataaaat
    17401 tcaggtagcc cgcctcgagg tccccgccgg ggccggcgtg cgccgccgcg aagccgtcca
    17461 ccgtgccccg gtacgccacc aggcctgagt ccagtaccgc cagctcgtcg ccgtgcttct
    17521 ccgcctcttc tagatactgc gtcgtgaaga agacgcacgc gccctcctcg gcgttcagcc
    17581 tgcgcatctc cgcccagaac gccgcgcggc tctcgggatc catcccgacc gacggctcgt
    17641 ccaaaaagag gacgcggggc cggtggacga gggccagcgc cacgtgtagg cgccgcttgt
    17701 tgccgccgga cagctcggct gcctttcgat cggcgacaag ctccagcccg aaccgcgcta
    17761 gcagttcttc tgcccgcccg gcggcccggg ccgcgtcgaa gccgtacagt cggccctgaa
    17821 acgctagctg ccggcgcgcg ctctcggctg ggtctagctg ggtatcctgg agcgccacgc
    17881 ctatcagggc gcggatagcc tgcgagccgc ccgagatcgg ccgccccgcg atcgcgacgc
    17941 gtcccgagtc ttggcgcgac agcgtgcaca ggatgcgcac gagcgtcgac ttgccggcgc
    18001 cattggcacc cagaagcgcg aagcggcagg cctcggggac gtcgaggtcg accccggcga
    18061 gggcccgagg cccct
//
LOCUS       DAOPHV010000003        16101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_4123_length_16101_cov_4.207217, whole genome shotgun sequence.
ACCESSION   DAOPHV010000003 DAOPHV010000000
VERSION     DAOPHV010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 16101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16101
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4123_length_16101_cov_4.207217"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PLQ29_00180"
     CDS             <1..656
                     /locus_tag="PLQ29_00180"
                     /inference="COORDINATES: protein motif:HMM:NF017106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HPG85088.1"
                     /translation="AMDSRSAAIKAALAAREEASAAKDAEIAADRESIAADQKGAIKG
                     EVSAAAAKDASGVPLFELVDPNLPFSRLALVDLKSGEVIRRSGINTIRAATALDLGDA
                     FVAIAGQVTGSGGAVRLVRVAKADYSKLTQGADDVFADSRLWKIGPSVYAVVKRGELW
                     AIGRFDPVTLELQAVSAPVSRWTFLTESGGRLVAQGPGGAFLVLEAEGLSTAAEIAR"
     gene            812..2389
                     /gene="pyrB"
                     /locus_tag="PLQ29_00185"
     CDS             812..2389
                     /gene="pyrB"
                     /locus_tag="PLQ29_00185"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HPG85089.1"
                     /translation="MTRNGRFKGRSIGVVGDLSVDEQRYLYRKARELKTELRAGGDAA
                     AFRIADPDYSAYLVFLENSTRTRESFRNAALFHRVRATMFDAATSSFAKNESLTDAIK
                     MLVGYAPESCFVIRSKLEGVCGWLADYLGPWAERSGYARPSFINAGDGKHEHPTQEFL
                     DEFSFLEQLGWRDERIHLALIGDLRHGRTVHSKADGLRVFKEAVVDLVAPPDLAMPPE
                     YVGKMRRNGFEVREWGSLDEYLASGKAAAIWYFTRLQLERMGESVLEKAPALRRAVTF
                     RRDMLDRLPEGARFYHPLPRDRLAPTIPGFLDDTVLNAWDAQSANGYYTRAVQMAMLA
                     GVLGDDFDGEGPAPRADDGAFVVEAAIEPRRKPEYKVGIKPVDSGLVIDHIASGASIE
                     EIWNRVDKIRRVLGLNLRSSHGVYHTNSGAYKGIVSVPDLLSFGEKELKRLGAVSPGC
                     TLNLVKDGAVVKKYRLGMPPRIYNFDDIACRNENCVSHPSHQEHVETSFVRKSGTDTF
                     VCRWCEREHSYSEIWRD"
     gene            complement(2386..3132)
                     /gene="rlmB"
                     /locus_tag="PLQ29_00190"
     CDS             complement(2386..3132)
                     /gene="rlmB"
                     /locus_tag="PLQ29_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPG85090.1"
                     /translation="MTYITGFHAMEEFVRSGKAGDATLLVAAAGPRIKKIIELADKAG
                     VGRKAVRREELDAAAPDHRGAAIALEVAPVAAEPSLEAWLETRRGPEAPARSVVVVLD
                     HIEDPHNLGAILRSADVFAADLVVVPERRAAKETDTVARSSAGAVAYVPTATVPNLAR
                     ALERLKEAGYWTYAADMGGQALPDADLPDRVALVLGAEGSGVSRLLKERCDGALSIPQ
                     YGHVDSLNVSVAAGVFMYELRRRHGAPPSA"
     gene            complement(3166..3987)
                     /locus_tag="PLQ29_00195"
     CDS             complement(3166..3987)
                     /locus_tag="PLQ29_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HPG85091.1"
                     /translation="MAVICIAREFAALGDETTKELAAMTGYKAIDKEYIEARMTEKGI
                     SAETRAKYDEKKPGFWASLSQDRDDYLHFLKTVLYEEAASGDCIIAGRGGFAIFAGVP
                     GCISVKLVAPRDVRLQRIRTRFQCDDKRAELLLRQNDRDRAGFHDYFFGVNWSDPVFY
                     DLTINTGREHPATVARIIDQLRSLIVSQEAEKACLGRMAELRLGQAIITEIVYARRVP
                     VHFVEADVKGSRVTLHGVANTQSAIDAASAAAGAVPGVETVDNAIQIVQEFAVMP"
     gene            4126..5019
                     /locus_tag="PLQ29_00200"
     CDS             4126..5019
                     /locus_tag="PLQ29_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPG85092.1"
                     /translation="MQRIGELAAFGTAICWTVSSMFFERATKRVGVLAVNFYKLVFAF
                     GFLVVAGAASRGMPLPLDATRDAWIFLSLSGLVGFVISDIFLFTAYKTIGSRITLLFV
                     ALSPPMTAALGFLFLGETMRPLAIVGMGLVVVGIVIAVLGRRDQAARTVMSRDDKRGY
                     VFALFSAIGQSVGMILTKRGIGDYDALSATQIRVLVGVVGFAVASVLAERGRNLKAAV
                     RDAPGMKSTFSGAIFGPFLGVVLSLYAVQQTNAGVVSTLMALSPVLIIPPAILVLKQK
                     VRFPEIAGAVIAVAGSAIFFL"
     gene            5069..6478
                     /locus_tag="PLQ29_00205"
     CDS             5069..6478
                     /locus_tag="PLQ29_00205"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPG85093.1"
                     /translation="MAITFYSEGAAREVTGSKHLLDVDGARYLVDCGAFQGKRDESDR
                     KNRELVADPASIEAVILTHAHYDHCGLLPLLSKRGFKGNVYSTPASRDLANLIMMDSA
                     RIQARDAQYLAKQAGKRGQTFDWKPLYDELDAIEAVGQFVTVSYERPMRIGRDVRMTF
                     RDAGHILGSATAHVTATDSSGRSVRIGFSGDLGRPDKPIIRDPQPMDPVDYLVIESTY
                     GDRRHESTTDAMDRLAEAVNVAVERGGKIIIPAFAIERTQELIYYFHLLVDQGRIPKI
                     PIWVDSPMAVNATSIFQVHPECYDLETHEAFIRHHNNPFGFNELKFSTSVDDSKALNR
                     LEGPAVIISADGMCEAGRIQHHLIHNISDERNTILVVGYMAANTLGRRIRDGQKEVRI
                     HGDLFKVKARVEEIKAFSAHADYQETWDWISRMDTSSLKTVFLVHGEDEALEAQKRFL
                     EAKGLRDVRIVEYGERYSL"
     gene            6713..7033
                     /locus_tag="PLQ29_00210"
     CDS             6713..7033
                     /locus_tag="PLQ29_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPG85094.1"
                     /translation="MPVTLINDTTIQTLLVVGPATVAEIDELGDAFRSAVASGAPTVR
                     VDLSGLGKLDVTFLQLLVSLRATLARAGRSLSCLGLGPDHPARRQAAALGLSVAFDGC
                     GGQR"
     gene            7030..9207
                     /locus_tag="PLQ29_00215"
     CDS             7030..9207
                     /locus_tag="PLQ29_00215"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HPG85095.1"
                     /translation="MNFEDNKGLFVEEARELLESIESRLLELEQAPGDEAIIDDVFRS
                     LHTVKGSGAMFGFEEMTAFAHGLESLFDDVRKGRLGVTGEIVGLTLESVDCLSSLLEG
                     RDDEAARARGSIESRLEAVRRGAEPAPEADAKPPAAAGSSATDKAGTAGGSKGIVPIA
                     ADDPSEAGLTNVYRIRFRPDADLLGRGVKLENLFSELAELGAVVAVADASRVPELDAL
                     EPSSLYLSWILTLSTTASEADVSSVFMFVEDYAELKIERLPVVDAEGKPAVPPIGELL
                     AARGALERDSIDEIRGRQKPFGELALEAGKVSKPELEAALAEQRMAKAAGQERENKKE
                     SASIRVRKEKLDYLVDAVGELVILQARLRQAATEAGLDEFSGIAEDLSRLTDELRDAT
                     MNIRMVPLDESFSGFPRLVHDLAAQLGKELTLTLRGGATELDKNIIESLKDPLVHIIR
                     NSADHGVESPEERERAGKPRAGTITIEARQQGSRVEIEIADDGAGLDLERVRERGVER
                     GLIDASERDPERIKSLIFEPGFSTARQTTGVSGRGVGMDVVRSNLRRLRGDVSIATER
                     GRGTAITLSIPLTLVIVDGLLVRVGGVHYVVNLSQVLECVDEPASGRAGRGARGFIDL
                     RGKTIPIVDLRERFGLGCGDERADRRLVIIDDDGSVVALWVDAVVDKQQVVIKPFTMD
                     LRQIASIAGATVLGDGSVALILNSREIIKTLRRDERAQTKECT"
     assembly_gap    9284..9383
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9384..10897
                     /locus_tag="PLQ29_00220"
     CDS             <9384..10897
                     /locus_tag="PLQ29_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG85096.1"
                     /translation="WLGFLSITALGVIIGVVGIINLNTMNMADTRLYEYATLPVQYAG
                     NIEAYIHRLRANLNTSFIETEPERVEYYRERRAGYFAEIEKNSDEYGKYIINDEEREL
                     YAKSLVLLAEFEKQDAAVQRLLEARDFEKAKAVLLKEYRASYDAYNEHSDKIVAFNVN
                     NAKETADGNTALALQSTIILIGVLGVSLAAALLLAWFLSGSITKVVDVIEDNALGVSD
                     GIAQVTGAAESLAQGSSEQAASVEEVSASIEELTATIRQNADNASQTEKIATKSAADA
                     REGGEAVTQTVRAMKDISERVLVIQAIATQTNLLSLNAAIEAARAGEHGRGFAVVASE
                     VQKLAERSSTAAKEIEEMSRSSVGIAEKAGTMLAELVPNIQKTADLVSEINAASGEQA
                     GGVQQINAAIQQLNSVIQENASGSEEMASTAEEISAQTMAMRDEVVFLKTGRRSGAEA
                     APAPVATVARKAPVAAKPAAKPVAKPAEAKPRGAKTAIVPLARDAEDDEFERF"
     gene            10900..11472
                     /locus_tag="PLQ29_00225"
     CDS             10900..11472
                     /locus_tag="PLQ29_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPG85097.1"
                     /translation="MSQANETDRAKAAGSHQYLRFTLAGEDYAFDVVKAREVLALVKI
                     TPLPCSMEHFAGVINLRGSIIPIMDLRKKFAMGSPAADFGSGAGAYIVVIEVGGQDGL
                     SVVGVLVDSVKSVVRFDDAELEPAPKFGMRVDSALIKAIAKRDELFITVLDSDRLFSE
                     KELSQVAAVESGPAAAEALAKARPKAEGEA"
     gene            complement(11481..12857)
                     /gene="creD"
                     /locus_tag="PLQ29_00230"
     CDS             complement(11481..12857)
                     /gene="creD"
                     /locus_tag="PLQ29_00230"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HPG85098.1"
                     /translation="MDGIRRITGSMGFKAALIAFLVLVMLIPMGLVRQVVGERADRAE
                     EAADEIVESWGGELRLAGPLLRIPCVRADEAIRRGDGGAETVERRTTAFDLWVGPTDL
                     GLKAEVASETRSRGVFGVPVFSGPVAFEGRFDAAEALGSLRDNEAAMLGEAELVMAIE
                     SQKGLRGIDGATWGGKPLAFRPGDAGLGLLSGGVKAAAPLESGAGADFALTASVQGGR
                     RLRLLPLGSSTSATVSSDWPAPSFQGEYLPASRGLGDDGFSATWRVSDLSRGIPSYWR
                     SGELPRGAMDRFYFGVDFFEALDRYALSERAAKHALLFVVVPFMTLFLLELFARRAVH
                     PIQYLLAGVANLVFYLLLLSLSEQVPFGAAYLIAASATGGLTFLYSWSTFKDLRKAWC
                     MAPVMGLSYLYLYAALRSEDSALLFGSIGAFAVTALVMFVTRDVDWYGYMKKAPGLAA
                     RGDDGASE"
     gene            13004..13711
                     /locus_tag="PLQ29_00235"
     CDS             13004..13711
                     /locus_tag="PLQ29_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPG85099.1"
                     /translation="MNRIAVVDDEKNIRETIRLALEREGYAVEEYANGEEAWAAFRRR
                     WPDLAILDIMMPRMDGMELCRRVRAESTMPIIFLSSRDDELDRVLGLESGGDDYVCKP
                     FGMRELTARVRAALRRASPEPGTGVAAAYGLRAGRLELDDERYLVRWDGREAALTVTE
                     FRMLWNLAELPGAVRTREQLMRAAFPEDAWPNDKAADSHIKRIRAKLSAVDPGFDGIE
                     TVYGLGYRLGGEGGRAR"
     gene            13708..15372
                     /locus_tag="PLQ29_00240"
     CDS             13708..15372
                     /locus_tag="PLQ29_00240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG85100.1"
                     /translation="MRRPFLGRLSVRLLAFNLLLVFLPLASLLYLDTYEKQLLEMQEA
                     SMIQQGRVFASAMAGVDLAAESTAILARLGARVDSRIRVVDTGGRLVADSAAEPAAAK
                     PRSAPGAEAQAASRKANERMLYRTFVSPINAVRRWLLPPGESYDSGEFYSGAATLLGP
                     EVQAALAGRYGSITRLSTGGQVSVNLYSAIPVFGSASNEVRGAVLVSRSTYGILRRLY
                     EVRLELIKVFVFSLAAALLLSVALSMTITVPIVRLKRQAEGVLDRSGRFRGRFTGLRR
                     KDEIGDLSRSLSDLSARLERRIGFIDGFTADLLHELKNPLAAIRGAVELALSEPGGDD
                     VGGDDDVGGDDAGDPAGDPASGLASGNARVRGLLLRVRDEELRMERLLSRLRELGRID
                     NRMDDEEASLVDLAELAPLILSRYPHKDYPTVRVVFDARVDGPALVRANPDRLTQLLS
                     NPVDNAVSFSPVGSTVTVRLSAGSLSGLSGYLVEVDDDGPGADPASLPRAFDRFYSDR
                     PAAAADEHTGLGLAIAHAIATGYGGACSLENRPEGGCRFSLVLPRA"
     gene            15451..15708
                     /locus_tag="PLQ29_00245"
     CDS             15451..15708
                     /locus_tag="PLQ29_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HPG85101.1"
                     /translation="MTISTIDDLSSMPEVELRALMARCGLDAIAFAVHGVDPATKRRL
                     IRALSPFRGLRLRAMRAYREAVPLASVEAAHAAIVAEANRA"
     gene            15759..>16101
                     /locus_tag="PLQ29_00250"
     CDS             15759..>16101
                     /locus_tag="PLQ29_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPG85102.1"
                     /translation="MRLSPLYRTVLAIAAPSVVELLLTSLTHLVDTIMVGRLGAYAIS
                     AVGLTTQPKFIMLAMFVALNVGSTALVARRYACSVAARYSAASRTQRMLSGIAATGGA
                     GPAAGAGSGAAD"
BASE COUNT         2564 a         5626 c         5353 g         2458 t
ORIGIN      
        1 ccgcgatgga ctcgcggtcg gcggctatca aggcggccct ggccgcacgc gaggaggcct
       61 ccgcagccaa ggacgccgag atagccgccg accgcgagtc catcgcggcc gatcagaagg
      121 gcgccatcaa gggcgaagta agcgccgccg cggccaagga tgcctccggc gtgcccctgt
      181 tcgagctcgt cgacccgaac ctgccgttct cgcgactggc cctcgtcgac ctcaagtccg
      241 gcgaggtgat caggcgtagc ggtatcaata cgatacgcgc cgcgacggcg ctcgacctcg
      301 gcgacgcctt cgtcgcgata gccggccagg taaccggctc gggaggagcg gtcaggctgg
      361 tccgcgtcgc caaggccgac tactccaagc tgacccaggg cgcggacgac gtcttcgccg
      421 actcgaggct ctggaagata ggcccgtccg tctacgccgt cgtcaagcgc ggcgagctct
      481 gggcgatagg gcgtttcgac cccgttaccc tggagctcca ggcggtttcg gccccggtca
      541 gccgctggac cttcctgacg gagtcgggcg ggcgcttggt ggcccagggg ccaggcgggg
      601 ccttcctggt attggaagcg gaaggcttgt ccaccgccgc ggagatagcg cggtgacgag
      661 gatggaaacg gaaggcagcg atgccttccg tttttttttg ccgttcgggt agggtgggcg
      721 ccggcttcgt cgagcgcgcc tcgataagca cggagcatga gccgcttcag ttcggatgca
      781 taaggttcgt agcactatgg aaggagcgcg tatgacgagg aacgggcgat tcaaggggcg
      841 gtcgatcggc gtggtggggg acctatcggt cgacgagcag cgatacctgt accgcaaggc
      901 tcgggagctg aagaccgagc tacgggctgg gggcgacgcg gcggccttca ggatagccga
      961 cccggattac tcggcctacc tcgtgttcct cgagaactcg acccggacca gggagagctt
     1021 ccgcaacgcg gcgctgttcc accgcgtccg ggccaccatg ttcgacgcgg ccacctcgag
     1081 cttcgccaag aacgagagcc tgacggacgc gatcaagatg ctcgtcggct acgcgcccga
     1141 gtcgtgcttc gtcatacgtt ccaagctcga gggcgtctgc ggctggctgg ccgactatct
     1201 cgggccctgg gccgagcgct ccggttacgc gaggccgtcg ttcatcaacg ccggcgacgg
     1261 caagcacgag cacccgaccc aggaattcct cgacgagttc agcttcctcg agcagctcgg
     1321 ctggcgcgac gagcgcatcc acctcgcgct gatcggcgac ctccgccacg gccgcaccgt
     1381 ccactccaag gccgacgggc tacgggtctt caaggaggcc gtcgtcgacc tggtggcgcc
     1441 gcccgacctg gctatgccgc ccgagtacgt cggcaagatg cgccggaacg gcttcgaggt
     1501 gcgcgagtgg ggctccctgg acgagtacct ggcctcgggc aaagccgcgg ccatctggta
     1561 cttcacgcgg ctgcagctcg agcgtatggg cgagtcggtg ctcgagaagg cgcctgccct
     1621 gcgccgggcc gtcaccttcc gcagggatat gctcgaccgc ctgcccgaag gcgcgcgctt
     1681 ctaccacccc ttgccgcgcg accgcctggc gccgaccatc ccgggcttcc tcgacgacac
     1741 ggtgctcaac gcctgggacg cccagtcggc caacggctac tacacccggg ccgtgcagat
     1801 ggccatgctc gccggcgtgc tgggcgatga tttcgacggc gaggggcccg ctccgcgggc
     1861 cgacgacggg gccttcgtcg tcgaggccgc catcgagccg aggcgcaagc ccgagtacaa
     1921 ggtcggcatc aagccggtcg actccggcct cgtcatcgac catatcgcct cgggcgcgtc
     1981 catcgaggag atctggaacc gcgtcgacaa gatacgccgc gtgctcggcc tcaacctgcg
     2041 ctcgtcgcac ggcgtctacc ataccaactc aggcgcctac aagggcatcg tgtcggtacc
     2101 cgacctcctg agcttcggcg agaaggagct caagcggctc ggggcggtat cgcccggctg
     2161 cacgctcaac ctcgtcaagg acggcgccgt ggtcaagaag taccgcctgg gcatgccgcc
     2221 gcgtatctac aacttcgacg acatagcctg ccgcaacgag aattgcgtgt cgcacccctc
     2281 gcaccaggag cacgtggaga cctcgttcgt ccgcaagagc ggcaccgaca cctttgtctg
     2341 ccgctggtgc gagcgcgagc actcgtactc ggagatctgg cgcgactagg cggaaggtgg
     2401 cgcgccgtgg cggcggcgca gctcgtacat gaacacgccg gcggccaccg agacgttgag
     2461 cgaatcgacg tgtccgtact gcggtatgga cagcgcgccg tcgcagcgct ccttgaggag
     2521 ccgcgagacg ccggagccct ccgcgccgag caccagggcc acgcggtcgg gcaggtcggc
     2581 gtcgggcagg gcctggccgc ccatgtcggc cgcgtaggtc cagtagccgg cctccttgag
     2641 ccgctcgagg gctcgggcca ggttcggcac ggtggccgtc ggcacgtacg ctaccgcgcc
     2701 ggccgacgag cgggctaccg tatcggtctc cttggccgcg cggcgctccg gtaccacgac
     2761 gaggtcggcg gcgaacacgt cggccgagcg caggatggcg ccgaggttgt gcggatcctc
     2821 tatatggtcg agtacgacga cgacggaccg cgccggcgct tccggcccgc gccgggtctc
     2881 cagccaggcc tccaggctgg gttcggcggc gactggcgcc acctcgagcg cgatggccgc
     2941 cccgcgatgg tccggggcgg ccgcgtcgag ttcctctcgc cgtacggcct tgcggccgac
     3001 gccggccttg tcggccagtt cgatgatctt cttgatgcgc ggcccggccg ccgcgacgag
     3061 cagggtggcg tcccccgcct tgccggagcg gacgaactct tccatggcgt gaaagccggt
     3121 gatgtacgtc atgcggcgat actatcccga gcgggccgtc cgagcctacg gcatcaccgc
     3181 gaattcctgc acgatctgga tcgcgttatc gaccgtctcg acgccgggta ccgcgccggc
     3241 ggccgcgctg gccgcgtcga tcgcgctctg ggtgttggcg acgccgtgca gcgtcacccg
     3301 cgagcccttg acgtccgcct cgacgaagtg gaccggaacg cgcctggcgt agacgatctc
     3361 ggtgatgatg gcctgaccga ggcgcagctc cgccatgcgc cccaggcagg ccttctcggc
     3421 ttcctgggag acgatcagcg accgcagctg gtcgatgatg cgggctaccg tggccgggtg
     3481 ctccctgccg gtgttgatcg tcaggtcgta gaagaccgga tcggaccagt tgacgccgaa
     3541 gaagtagtcg tggaagccgg cgcggtcgcg gtcgttctgc ctgagcagca gctcggcgcg
     3601 cttgtcgtcg cactggaagc gggtgcgtat gcgctggagc cggacgtcgc gcggcgccac
     3661 gagcttgacc gagatgcagc ccggtacgcc cgcgaagatg gcgaagccgc cgcggccggc
     3721 tatgatgcag tcgccggagg ccgcctcctc gtacaggacc gtcttgagga agtgcaggta
     3781 gtcgtcgcgg tcctgggaca gcgaggccca gaagccgggc ttcttctcgt cgtacttggc
     3841 ccgggtctcg gcggagatgc ccttctccgt catccgcgcc tcgatgtact ccttgtcgat
     3901 agccttgtag ccggtcatcg cggccaattc cttggtcgtc tcgtctccaa gcgcggcgaa
     3961 ttccctagct atacaaatga ctgccatggg gcctccttcc gggaaccggc tagccgttcc
     4021 cccgcctacg ctaaccatag gaccgtccgc ccgaattgtc aaatcgcggc ggcccgggcg
     4081 ccctggcttc ccacgcgcgg cccgcgtcgg tatagtcgag ccatcatgca gcgaatcggc
     4141 gaactggcgg ccttcggtac ggccatctgc tggaccgtat catcgatgtt cttcgagcgg
     4201 gcgacgaagc gcgtcggcgt cctggccgtc aacttctaca agctcgtctt cgcgttcggc
     4261 ttcctcgtcg tcgccggcgc cgcgtcgcgg ggcatgcccc tgccgctcga cgcgacgagg
     4321 gacgcctgga tcttcctctc gctgtcgggc ctcgtgggct tcgtcatctc cgacatcttc
     4381 ctgttcaccg cctacaagac cataggctcg cggatcacgc tgctgttcgt cgccctatcg
     4441 ccgcccatga ccgccgccct gggcttcctg ttcctcggcg agacgatgcg gccgctggcc
     4501 atcgtcggca tgggcctggt cgtcgtcggc atcgtgatcg ccgtgctcgg ccggcgcgac
     4561 caggcggcca ggaccgtgat gagccgcgac gacaaacggg gctacgtctt cgcgctgttc
     4621 tcggccatag gccagtcggt cggcatgatt ctcacgaaac gcggcatcgg cgactacgac
     4681 gccctgtccg ccacccagat ccgcgtgctc gtcggcgtcg tcggcttcgc cgtcgcttcc
     4741 gtcctggccg agcgcggccg caacctcaag gccgccgtcc gcgacgcgcc tggcatgaag
     4801 tccaccttca gcggcgccat cttcgggccc ttcctcggcg tcgtgctgtc gctgtacgcg
     4861 gtgcagcaga ccaacgccgg cgtcgtcagt acgctcatgg cgctgtcccc ggtcctgatc
     4921 atcccgccgg ccatactcgt gctcaagcag aaggtgcgtt tccccgagat agccggggcc
     4981 gtcatcgccg tggccggctc cgccatcttc ttcctctagg ctatcgcgca ggcttgcgga
     5041 tagccagaaa ccggcctatc ctgtacctat ggctatcacc ttctattccg agggagccgc
     5101 ccgagaggtc accggctcga agcacctgct cgacgtggac ggagcgcgct acctggtcga
     5161 ttgcggcgcc ttccagggca agcgcgacga gagcgaccgc aagaaccgcg agctcgtggc
     5221 cgaccccgcc tcgatcgagg ccgtcatcct gacccacgcc cactacgacc actgcggcct
     5281 cctcccgctc ctttccaagc gcggcttcaa gggcaacgtc tactcgaccc ccgcctcccg
     5341 cgacctggcc aacctgatca tgatggactc cgcgcgcatc caggcgcgag acgcccagta
     5401 cctggccaag caggccggca agcgcgggca gaccttcgac tggaagcccc tgtacgacga
     5461 gctcgacgcg atcgaggcgg tcggccagtt cgtcaccgtc tcgtacgagc ggccgatgcg
     5521 gatcgggcgg gacgtgcgga tgaccttccg cgacgccggc catatcctcg gctcggccac
     5581 cgcccacgtg accgcgaccg actcctccgg ccgctccgtg cgtatcggct tctccggcga
     5641 tctcgggcgc ccggacaagc ccatcatccg cgacccgcag cccatggacc cggtcgacta
     5701 cctcgtcatc gagagcacct acggcgatcg ccgccacgag tccacgaccg acgcgatgga
     5761 ccggctggcc gaggcggtca acgtcgccgt cgagcgcggc ggcaagatca tcatcccggc
     5821 cttcgccatc gaacgcacgc aggagctcat ctactacttc cacctgctcg tcgaccaggg
     5881 ccgcataccc aagatcccta tctgggtcga ctcgccgatg gccgtcaacg ccacgagcat
     5941 cttccaggtc cacccggaat gctacgacct cgagacccac gaggccttca tccgccacca
     6001 caacaacccc ttcggcttca acgagctcaa gttctccacg agcgtcgacg actccaaggc
     6061 gctcaaccgc ctggagggcc cggccgtcat catatcggcc gacggcatgt gcgaggccgg
     6121 ccgcatccag caccacctga tccacaacat ctccgacgag cgcaacacga tactcgtcgt
     6181 cggctacatg gccgccaata ccctcggtcg ccgcatccgc gacgggcaga aggaggtccg
     6241 catccacggc gacctcttca aggtcaaggc ccgcgtcgag gagatcaagg ccttcagcgc
     6301 ccacgccgac taccaggaga cctgggactg gatcagccgg atggacacct cgtcgctcaa
     6361 gaccgtgttc ctggtccacg gcgaggacga ggcgctcgag gcgcagaagc gcttcctcga
     6421 ggctaagggc ctccgggacg tgcgcatcgt ggagtacggc gagcgctata gcctctagga
     6481 gccgcccctg gcgacgatat gccgcaccgg cgcgctccgc gctccggccg cctcctgtac
     6541 cggtcgcagc gctggcggcc gcctcgccgc cgccagggac gggcctccac tcccgccccg
     6601 cgcctgggct gagaacgcct cgatgtgaat ggattaaatt aatatatcgt gttaaataaa
     6661 taaattttac attatagtta caagttacgt ataggtgata tacgataccg gtatgccagt
     6721 aactttaatt aatgatacga ctatccaaac cctgctggtg gtcggtccgg cgaccgtcgc
     6781 cgagatcgac gagctcggcg acgccttccg ctcggccgtc gcctcgggcg ccccgacggt
     6841 tcgggtcgac ttgtcaggct taggcaagct cgacgtcacc ttcctgcagc tcctcgtatc
     6901 gctccgcgcc acgctggccc gtgccggccg gtcgctgtcc tgccttggcc tgggacccga
     6961 ccatccggcg cgccgccagg ccgccgccct cggcctttcg gtcgccttcg acggctgcgg
     7021 aggccagcga tgaacttcga ggacaacaag ggcctcttcg tggaggaagc gcgcgagctg
     7081 ctcgagtcca tcgagtcgag gctgctcgag ctcgagcagg cgcccggcga tgaggccatc
     7141 atcgacgacg tgttccgctc gctccatacc gtcaagggct cgggcgccat gttcggcttc
     7201 gaggagatga ccgccttcgc ccatgggctc gagagtctgt tcgacgacgt gcgcaagggg
     7261 cggctcggcg tcacgggcga gatcgtcggc ctgaccctcg agtcggtcga ctgcctgtcg
     7321 tcgctgctcg agggccgcga cgacgaggcg gccagggcgc ggggctcgat agagtcgcgt
     7381 ctggaagccg tcaggcgcgg agccgagcct gcgcccgagg cggacgccaa gcccccggcc
     7441 gcggccggct cgagcgcgac ggacaaggcc ggaaccgcgg gcggttccaa gggcatcgtg
     7501 ccgatagccg ccgacgaccc gtccgaggcc ggcctcacga acgtgtaccg catccgcttc
     7561 cggccggacg ccgacctcct cgggcgcggc gtcaagctgg agaacctgtt ctccgagctg
     7621 gccgagctgg gcgccgtcgt cgccgtcgcg gacgcctcgc gggtgcccga gctcgacgcg
     7681 ctcgagccgt cgtcgctcta cctgtcctgg atcctcaccc tgtccacgac ggcgagcgag
     7741 gcggacgtct cgtcggtctt catgttcgtc gaggactacg ccgagctcaa gatcgagcgg
     7801 ctgcccgtgg tcgacgcgga gggcaagccc gccgtgccgc cgatcggcga gctcctggcc
     7861 gcccggggcg cgctggagcg cgacagcatc gacgagatcc gcggccggca gaagcccttc
     7921 ggcgagctgg cgctcgaggc gggcaaggtc agcaagcccg agctcgaggc cgcgctcgcc
     7981 gaacagcgca tggccaaggc cgcgggccag gagcgcgaga ataagaagga atcggcgagc
     8041 atccgggtcc gcaaggagaa gctcgactac ctggtggacg ccgtcggcga gctcgtcatc
     8101 ctccaggccc gcctgcgcca ggccgcgacc gaggccgggc tggacgagtt ctccggcatc
     8161 gccgaggacc tgagccggct gaccgacgag ctgcgcgacg cgacgatgaa tatccgcatg
     8221 gtgccgctcg acgagtcgtt ctcgggtttc ccgcgcctcg tccacgacct ggcggcccag
     8281 ctcggcaagg agctgaccct gaccctgcgc ggcggggcca ccgagctgga caagaacatc
     8341 atcgagtcgc tcaaggaccc gctcgtgcac atcatccgca actcggccga ccacggcgtc
     8401 gagtcgccgg aggagcgaga gcgagccggc aagccgcggg ccggtaccat taccatcgag
     8461 gcccgccagc agggctcccg cgtggagata gagatagccg acgacggcgc cggcctggac
     8521 ctggagcgcg tacgcgagcg cggcgtcgaa cgcggcctga tcgacgcgtc cgagcgcgac
     8581 cccgagcgca tcaagtcgct gatcttcgag ccgggcttct ccacggctcg ccagaccacc
     8641 ggcgtctcgg gccgcggcgt cggcatggac gtggtccgga gcaacctccg gcgcctgcgc
     8701 ggcgacgtgt ccatagccac ggagcggggc cggggcaccg ccatcaccct gtccatcccg
     8761 ctgaccctcg tgatcgtcga cggcctcctc gtgcgcgtcg gcggggtgca ctacgtcgtc
     8821 aacctgagcc aggtgctcga atgcgtcgac gagccggcct ccgggcgggc gggccgcggg
     8881 gccaggggct tcatcgacct gcgcggcaag accatcccga tcgtcgacct gcgcgagcgc
     8941 ttcggcctcg gctgcggcga cgagcgggcc gaccggcgcc tggtgatcat cgacgacgac
     9001 ggctccgtgg tcgccctctg ggtggacgcg gtcgtcgaca agcagcaggt ggtcatcaag
     9061 ccctttacca tggatctgcg ccagatagcc agcatagccg gcgccaccgt gctcggcgac
     9121 ggctcggtgg ccctcatact caactcgaga gagatcatca agaccctgag gcgcgacgaa
     9181 cgcgcccaga caaaggagtg cacatgaacc tcggaatccg cgcgaagctc tggctgggct
     9241 tcctgtccat cacggccctc ggcgtcatca tcggcgtggt cggnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnntctggct gggcttcctg tccatcacgg ccctcggcgt
     9421 catcatcggc gtggtcggca tcatcaacct gaacacgatg aacatggccg acacgagact
     9481 gtacgagtac gcgaccctgc cggtccagta cgcgggcaac atcgaagcct acatccatcg
     9541 cctccgcgcc aacctgaaca cctcattcat cgagaccgaa ccggaacgcg tcgagtacta
     9601 cagagagcgg cgggccggct acttcgccga gatcgaaaag aacagcgacg aatatggcaa
     9661 gtacataatc aacgacgagg aacgcgaact ctatgcgaag tccctcgtgc tgctggccga
     9721 attcgagaaa caggacgccg cggtccagcg cctgctcgag gcccgcgact tcgagaaggc
     9781 caaggccgtg ctcctcaagg agtatcgggc ctcctatgac gcctacaatg agcattccga
     9841 taagatagtc gcgttcaatg tcaacaacgc caaggagacc gccgacggca acaccgccct
     9901 ggcgctccag tcgacgatca tcctcatcgg ggtcctcggc gtcagcctgg cggcggccct
     9961 gctcctggcc tggttcctga gcggctcgat cacgaaggtg gtcgacgtga tcgaggacaa
    10021 cgccctgggc gtgagcgacg gcatcgcgca ggtgacgggc gcggcggaga gcctggccca
    10081 gggctcgagc gagcaggccg cgagcgtgga ggaggtgtcc gcgagcatcg aggagctgac
    10141 cgcgacgatc aggcagaacg ccgacaacgc tagccagacc gagaagatag cgaccaagtc
    10201 ggcggccgac gcccgcgagg gcggcgaggc cgtcacgcag acggtccgcg cgatgaagga
    10261 catctccgag cgcgtgctcg tgatccaggc catagccacc cagacgaacc tcctgtcgct
    10321 caacgccgct atcgaggccg ccagggccgg cgagcacggc cgcggcttcg cggtggtggc
    10381 gagcgaggtg cagaagctgg ccgagcgcag ctcgacggcg gccaaggaga tcgaggagat
    10441 gtcgcgctcg agcgtcggca tagccgagaa ggccggaacg atgctcgccg agctcgtgcc
    10501 gaacatccag aagaccgccg acctcgtctc cgagatcaac gcggcctccg gcgagcaggc
    10561 cggcggcgtg cagcagatca acgcggccat ccagcagctc aactccgtca tccaggagaa
    10621 cgcctccggc tccgaggaga tggcctcgac cgccgaggag atatccgccc agaccatggc
    10681 catgcgcgac gaggtggtct tcctcaagac cggtcgcagg tcgggcgccg aggcggcccc
    10741 ggccccggtg gcgaccgtgg cccggaaggc tccagtagcg gccaagccgg ccgccaagcc
    10801 ggtcgccaag cccgccgagg ctaagcccag gggcgccaag accgccatcg tacccctggc
    10861 gcgcgacgcc gaggacgacg aattcgagag gttctgagca tgagccaagc caacgagacc
    10921 gatcgcgcca aggcggccgg ctcccaccag tacctacgct ttaccctggc gggcgaggac
    10981 tacgccttcg acgtggtcaa ggcccgcgag gtgctggcgc tggtcaagat caccccgctg
    11041 ccctgctcga tggagcactt cgcgggcgtg atcaacctgc gcggcagcat catccccatc
    11101 atggacctgc gcaagaagtt cgccatgggc tcgccggcgg cggacttcgg ctcgggcgcc
    11161 ggggcctaca tcgtcgtgat cgaggtgggc ggccaggacg gcctgtcggt ggtcggcgtg
    11221 ctggtcgact cggtcaagag cgtcgtgcgc ttcgacgacg ccgagctcga gccggctccc
    11281 aagttcggca tgcgcgtcga ctcggcgctg atcaaggcga tagccaagcg cgacgagctg
    11341 ttcatcacgg tgctcgactc cgaccggctg ttctccgaga aggagctcag ccaggtcgcg
    11401 gccgtcgagt ccggcccggc cgccgccgag gccctggcca aggctcggcc gaaggccgag
    11461 ggcgaggcgt agcccgagcc ttactccgag gctccatcgt cgccccgggc cgcaaggccc
    11521 ggggctttct tcatatagcc gtaccagtcc acgtccctcg tcacgaacat gacgagcgcg
    11581 gtcaccgcga acgcgcctat cgagccgaag agcagggcgc tgtcctccga gcgcagggcg
    11641 gcgtacaggt acaggtacga aagccccatc accggagcca tgcaccaggc cttgcggaga
    11701 tccttgaacg tcgaccacga gtataggaag gtcaggccac ccgtcgccga ggccgcgatc
    11761 aggtaggccg ccccgaacgg gacctgctcc gacagcgaca ggagcaggag gtagaagacc
    11821 aggttcgcca cgccggccag caggtactgg atcggatgca cggcccgacg cgcgaacagc
    11881 tcgagcagga acagcgtcat gaacggcacg acgacgaaca acagggcgtg cttcgcggcc
    11941 cgctcggaaa gcgcgtagcg gtcgagcgcc tcgaagaagt cgacgccgaa gtagaagcgg
    12001 tccatcgcgc cacgcgggag ctcgcccgag cgccagtacg acgggatgcc gcggctcagg
    12061 tcgctgactc gccaggtggc ggagaagccg tcgtcgccga gaccacggct ggccggtagg
    12121 tactcgccct ggaacgacgg ggcgggccaa tcggacgata cggtcgcgga cgtcgacgag
    12181 ccgagcggca gtagcctgag ccggcggccg ccttgcaccg aggccgtcag cgcgaagtcc
    12241 gctccggcgc cggattcgag cggagccgcc gccttgacgc cgcccgacag gaggccgaga
    12301 ccggcgtcgc cgggcctgaa ggcgagcggc ttcccgcccc acgtggcgcc gtcgataccg
    12361 cgcaggccct tctggctttc gatggccatg accagctcgg cctcgccgag catggcggcc
    12421 tcgttgtcgc gcaggctacc gagcgcctcg gccgcgtcga agcggccctc gaaggctacc
    12481 gggccggaga acaccggcac gccgaagacg cctcggctcc tcgtctcgct cgcgacctcg
    12541 gccttcaggc cgaggtcggt cgggcctacc cagaggtcga aggccgtagt ccggcgctcg
    12601 acggtctccg cgccgccgtc tccgcgccgg atcgcctcgt cggcgcgtac gcagggtatc
    12661 ctgaggaggg ggccggccag cctgagctcg ccgccccagg actcgactat ctcgtccgcg
    12721 gcctcctcgg cgcgatcggc tcgctcgccg acgacctgcc tgaccaggcc catcgggatc
    12781 agcatgacca ataccaaaaa ggcgatgagg gccgccttga aacccatgct gccggtgatt
    12841 cttcgtatac cgtccatagt ggctccttcc gggggacttg cgagaggcta ccccgccaat
    12901 cgggccgcag ggtggccgga aggtggcagc attccggctt ccgcccggtt cccggccgtc
    12961 cggggattca cgcggccgac gggccgtgct atcctcgcgg ttgatgaatc gcatagcagt
    13021 cgtcgacgac gagaagaaca tacgcgagac gatacggctg gccctcgagc gcgagggcta
    13081 cgccgtcgag gagtacgcca acggcgagga ggcctgggcg gccttcaggc gccgctggcc
    13141 cgacctggcc atcctggaca tcatgatgcc caggatggac ggcatggagc tgtgccggcg
    13201 cgtgcgggcc gagtcgacga tgcccatcat cttcctgtcg tcgcgcgacg acgagctgga
    13261 ccgggtgctc ggcctcgagt cgggcggcga cgactacgtg tgcaagccct tcgggatgcg
    13321 ggagctgacc gccagggtgc gcgccgccct gcgccgagcc tctccggagc ctggaaccgg
    13381 ggtcgccgcc gcgtacggcc tgcgggcggg caggctggaa ctcgacgacg agcgctacct
    13441 ggtgcgctgg gacggccgcg aggcggcgct caccgtgacc gagttccgca tgctctggaa
    13501 cctggccgag ctaccgggag cggtgcgcac gcgcgagcag ctgatgcggg ccgccttccc
    13561 ggaggacgcc tggccgaacg acaaggcggc cgacagccat atcaagcgga tccgagccaa
    13621 gctgtccgcc gtcgacccgg gcttcgacgg catcgagacg gtctacgggc tcggctatcg
    13681 cctgggcggc gaaggcggcc gggcgcgatg aggcggccgt tcctcgggcg gctctcggtc
    13741 cgcctcctgg ccttcaacct gctgctcgtc ttcctgccgc tcgccagcct cctgtatctg
    13801 gacacctacg agaagcagct cctggagatg caggaggcct cgatgatcca gcagggccgc
    13861 gtgttcgcct cggcgatggc gggcgtcgac ctcgcggccg agtcgacggc gatcctggcc
    13921 cggctcggcg ccagggtcga ctcgaggatc agggtggtcg acacgggtgg gcgcctcgtc
    13981 gccgactccg ccgccgagcc ggccgccgcg aagccgcgct cggcgccggg cgccgaggcc
    14041 caagccgcct cgcgcaaggc taacgaacgc atgctgtacc gaaccttcgt ctcgccgatc
    14101 aacgccgtca ggcgctggct cctgccgccc ggcgagtcgt acgattccgg ggagttctac
    14161 tccggcgccg cgaccctgct cgggcctgag gtccaggcgg cgctggcggg ccgctacggc
    14221 tcgatcaccc gtctctcgac cggcggccag gtgtcggtca acctgtactc ggccataccg
    14281 gtcttcggtt ccgcctcgaa cgaggtacgg ggagcggtgc tcgtctcgcg ctcgacctac
    14341 ggcatactca ggcggctcta cgaggtccgc ctcgagctca tcaaggtgtt cgtcttctcg
    14401 ctcgccgcgg ccctcctcct gtccgtagcg ctgtcgatga ccatcacggt gccgatcgtg
    14461 cggctcaagc gccaggccga gggcgtgctc gaccgctcgg ggcgtttccg tggccgcttc
    14521 accggcctac ggcgcaagga cgagataggc gacctgagcc gttccctgtc cgacctgtcc
    14581 gcgcggctcg agcgccgcat cggcttcatc gacggcttta ccgccgacct gctccacgag
    14641 ctcaagaacc cgctggcggc tatacgcggc gcggtcgagc tggccctgtc cgagccgggc
    14701 ggcgacgacg ttggcggcga cgacgacgtt ggcggcgacg acgccggcga tccggctggc
    14761 gaccctgcga gtggcctcgc gtccgggaac gcccgcgtgc ggggcctgct cctgcgggtg
    14821 cgcgatgagg agctgcgcat ggaacggctc ctgtccaggc tgcgcgagct ggggcgcatc
    14881 gacaaccgga tggacgacga ggaggcctcg ctcgtcgacc tggccgagct cgcgccgctc
    14941 atcctgtcgc gctatcccca caaggactac ccgacggtcc gggtggtctt cgatgcgcgg
    15001 gtcgacggcc cggctctggt ccgcgccaac cccgaccggc tgacgcagct cctgtccaat
    15061 ccggtggaca acgccgtgtc cttttcgccg gtcggctcga ccgtgaccgt caggctgtcg
    15121 gctggctccc tgtccgggct atccggttac ctcgtcgagg tggacgacga cgggccgggc
    15181 gccgatccag cgtccctgcc ccgcgcgttc gaccgcttct actcggaccg cccggcggcc
    15241 gccgccgacg aacataccgg gctgggcctg gccatagccc acgccatagc cacgggctac
    15301 ggcggcgcct gctcgctcga gaaccgaccc gagggcggtt gccgcttcag cctggtcctg
    15361 cctcgcgcct aagcgcccgc ttgcggccgg acggcgacga gcctatgctt atgaggttcc
    15421 gctccagcgc gggaaccgag gagggaaagc atgacgattt caacgatcga cgatctttcg
    15481 tccatgcccg aggtcgagtt acgcgccctg atggccaggt gcgggctcga tgccatcgcc
    15541 ttcgccgtgc atggtgtcga ccccgccacg aaacgccgcc tgatcagggc gctgagcccg
    15601 tttcgtgggc tcaggctgcg cgcgatgagg gcctatcggg aagccgtgcc gctcgcctcg
    15661 gtcgaggcgg cccacgccgc gatcgtggcc gaggctaacc gcgcctaagc gcccgcttgc
    15721 cgcgccgagc gcttcggcta tatagtcaag cattgtctat gcgcctttcc cccctgtacc
    15781 ggaccgtcct ggctatcgcc gctccgtctg tagtcgagtt gctcctcacc tcgctgaccc
    15841 acctggtcga caccatcatg gtgggccgcc tcggcgccta cgccatttcg gccgtcggcc
    15901 tgaccacgca gcccaagttc atcatgctcg ccatgttcgt ggcgctcaac gtcggctcga
    15961 cggcgctcgt cgccaggcga tacgcctgca gcgtggcggc gcggtactcc gcggcgtcgc
    16021 gcacccagcg gatgctgtcg ggcatcgccg cgaccggcgg tgccggtccc gcggcgggcg
    16081 cgggcagcgg cgcggcggat c
//
LOCUS       DAOPHV010000004        13743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_5203_length_13743_cov_4.227572, whole genome shotgun sequence.
ACCESSION   DAOPHV010000004 DAOPHV010000000
VERSION     DAOPHV010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 13743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13743
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5203_length_13743_cov_4.227572"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2831
                     /locus_tag="PLQ29_00255"
     CDS             <1..2831
                     /locus_tag="PLQ29_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPG85103.1"
                     /translation="SSITITFDWEIDGDAARDQVVAAMNGVRSYLPEDLRDAYSIRFR
                     EGENAGFIVMGVVSNATPPEELMPMLQAGVESRLATIDGVDEYGIYGLEELSVEVTLD
                     HEAMLAAGVSMLDVDAAFRAGLSPSPLGTLREGDERYSLRLRRPTAGLESLGRLEIAR
                     DGLAVTRLEDIAEIDVRYDLPSRVFLLGDKPAIQITLTPEDGGNLNRMTEELVVIMEE
                     AKASGRLPADVGFELYLDPARYINRSISNVVQSALLGGLLAVLIVFLILGELRNTLII
                     ALTVPVTIVLSFILMSLFGLTINLISLGGLALAVGMIVDPTIVVMENIHRWRAEARRA
                     GDASGSAAGKDLVADATRQVRGPVIASALTSVAVFLPLTFTAPLAAAILGDQAKTVVF
                     SLLVSLLVSLSFVPVAAYLLFGTGKRAALSEPRGYAKIADAAMDRLVEAYRRALRAVI
                     RTRPRAATFLTLAFAALVVSVGLLLPRVPKEMLPTPSSDRVVVFFRHGDYTTAPEVIE
                     RLMPELRERIAGAMDPIAYKDFASVSGRMNQIFVDLESTEYTDEAIVRLEAEFESEGQ
                     WYFSIASWDPAALPLPQTNDLRLSVHGPDPTVKVALLDEMQRALNDSRLYGRTYARPS
                     PALTREVALETRPGAFDGFPGLGEASMATLVRRSLAGSAGLSLSDGSSEVSVSATYPD
                     GLLDSRDELARFLVPWKGTGVPLKHFFDFEESETVSQVYSENGEAAYRLYANISGEAS
                     DAVRLEKEAAARRLFDSAIEPPAGYGYSFDNPRGELDEAISSLLAALAISIGLVYLLL
                     AFQYDSFGVPLAILVAVPFGFVGVALSLFAFRSTLNLNSLLGTILLCGVVVNNSIIMI
                     DFYLKTRKNHQDNASAIEAAAALRLKPILITTLTTVFGMLPIALGMGSGSNILQPLGI
                     AVSGGLAISTLLTLFAVPALLRFWRFPA"
     gene            complement(2841..4109)
                     /locus_tag="PLQ29_00260"
     CDS             complement(2841..4109)
                     /locus_tag="PLQ29_00260"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPG85104.1"
                     /translation="MRFLPKRYLEEFFLYSSQFVLFFIIILFTSPAIIRPSTSALISL
                     IVFLLLQIALLARFGHRPPARFFFSLMTPLGYTTILTLSKSAQGIDMASFFLWATSLY
                     LGGIQALAILFRRKWPKRIAEIFLSLGTILSFIFFYFYLDLRLTSTKAYEAGELGYAA
                     LVSSLKLSNFGPAFGEFAKTLQHVFFMFGAVMFGSFQVADKIKQVSMKARLDQVYSIV
                     EPPSAEPSMFDQVSDGENREVVILYADLWNFTPIAEKEGARATIGLLNRYYALWDIVA
                     RKHGGVVEQFVGDTAIVVFGLMGEKDAADQAVSCAFDFLYEFSHLQEDLAAHRLPLVK
                     HIGIGVHAGKVAVGELSSASGVQRVGVVGEAVNIAARLDSLCREFKQDLLISQPVYKQ
                     LSLEPQSRFQPMGEVLFRGKTQPQAVYGKK"
     gene            4301..5629
                     /locus_tag="PLQ29_00265"
     CDS             4301..5629
                     /locus_tag="PLQ29_00265"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPG85105.1"
                     /translation="MTALSTIVAACSRSAETTDADGPSLPGGIDGRAAFSAIEALTGP
                     LYAGRIVGGDGSAAARAYLRARLEAVGLAVEGLEFIERVALNDGEAFLRARTPGGATT
                     VFEYRSDFREAPINGFDGGRASGPLKRLTGSGFEPGTILLAPKEARPSEIAAGLAAAG
                     AAGLLFELDKSSPALRPLWPGHEPGALVKVKHGMPILGLSGEAFAALAAMVATAGADP
                     GADAPFIELASPVRFADVIGANLLAVWNGDGGSFEPAYLVMAHYDHVGADPDGSYFPG
                     ALDNASGVGVALALAEAAASYRPRVDLAFLFTDAEEGGLGGSTAFVSAPPFPLGGASV
                     INLDMLGSCAETRYSVYSSGGQAGVSLSLRVERALSSGGIPATSEHPVDVSDHAPFAS
                     AGVAAVTVCEYDTEHYHRKDDLPEYLDTDELDAVGDALYRLITTAAVAGP"
     gene            complement(5590..6687)
                     /locus_tag="PLQ29_00270"
     CDS             complement(5590..6687)
                     /locus_tag="PLQ29_00270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG85106.1"
                     /translation="MKRLAVMLSRGYVLATSAALLAVFMVIGYPALGSSLAAATPDGG
                     AFDLSLFYTPAEAVRKASSYTEAEVRASIRVHWTLDLVFPLAYGLFCASAWAFGLRRL
                     ARPASEPRYGFLLVPLASTAFDLAENVAVAALLVGARQSAPATPGIAAVCASVATTLK
                     WAFAGLAFVGAPCLLVAGAFAGRARGRRASARVVMKRAGLDDLPAVMRLEDAGFGMGI
                     REHAEVFRARMELFPEGFVALRRGGETVGYLCAERWAEVPPAEARWFELGHDPASRHD
                     PDGSVLYLASMTIDPALRGTGLGSRLFNDGIAMIRAAASGIETEVLIVNEAWTGARRI
                     YEAAGFASYGVIDSFFPNLYGPATAAVVMRR"
     gene            6921..9404
                     /locus_tag="PLQ29_00275"
     CDS             6921..9404
                     /locus_tag="PLQ29_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPG85107.1"
                     /translation="MCGIVSLVYKDETRNMGREAAELLRRLEYRGYDSTGASFIGKDR
                     NIVLVKRVGSPTRVCAELNVPSYKGQRFIGQVRWATYGAVSDVNSQPHHVRCKVEMVG
                     AHNGNISNTDSLQASLSARGHKVVSDNDGEIIVHLVEDHYAANKAAPQKALAEMRRAY
                     AASGLSAPVADEVLLIVDAIRKAEAEADGSYAAAVADPQVPGCFAIKSGPSLYAGLGK
                     DEDGSFVVVSSDLTSVIAKTPMLIPLAEGEGLWYTEGRYLVFSLGGEARFAEPRLKRS
                     KLNVRDTGLNPRYKYYMEQEIASAPDNMDAIERYYFADEREAGLASVLEDNRSVAKEL
                     MDKACDLASAANDAALGAAFDELAASSDWRALAAKVNADAAARAPLAGRTSFRSDEAQ
                     LLTDLARLRPAAAADLALLDLAMIWRKRRSVTRYARELSDRIRETRKAGGTVYVVASG
                     TSYHASLTAAYFWNGLAGVPLYPCNPGQFRSAYMDSLGDADLILAVSQSGETKDLVDI
                     LQDVAETRPRIKRAALVNNENSRIPQELADFYLPILCGPEIAVAATKSFVNQLVIMYI
                     LAASYKQPEAAVKASVNRAKGLLSDTLRLSAPAIDEVAERLYLKPSIHMLGTGLFGLA
                     REAALKVREVVLNHSEGYDAAEFKHGPNTILGKNTVFSIAEVVKAMDAALAAGTAGTG
                     AGTGAGKSAAELLAADPSIVEGLFSNYPLVFVCPPDARDARITISQIHTHKIRGADIV
                     LFAEKHPELDLAVKGVPSGRDDYRSAYVEIPASGDRYLFVFAATVALQYLAFRMSVLK
                     MEYLDRLGVRDHGVHPDAPKNVSKSITVD"
     gene            complement(10253..10954)
                     /locus_tag="PLQ29_00280"
     CDS             complement(10253..10954)
                     /locus_tag="PLQ29_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPG85108.1"
                     /translation="MIKRTNDSVPTVAPMVPRSSAQMAAPVGLWLAFILFTVAVGTVD
                     REAIGPDGSVVGLAAMNRLIYELLEAQPFWYALTDWLGLVPVAVACGFACLGAWQLVR
                     RRSLRRVDADIMLLGALYLAVVGYYVLFERLVVNYRPILIGGRLEASYPSSHTMVAVG
                     VMATAVLQFRRRTRSRRLLAALELASAAVIAVTALGRLISGAHWLSDIIGGLLLGSAL
                     AASYRAALARGRPVS"
     gene            complement(11039..11644)
                     /locus_tag="PLQ29_00285"
     CDS             complement(11039..11644)
                     /locus_tag="PLQ29_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPG85109.1"
                     /translation="MEFNEKLRSLRARAGLTQERLADELCVSRVTVSKWESGRGYPNI
                     ESLRLMAKTLSVSIDDLLSGDELLSVAESQSRESSGGLLAVAFGVLDVMMTLLLALPL
                     FGDEIGGRIASVALPRVTGMPPDIVAAYYVAVVATATFGVLQLALQNLRSPAWRRLSL
                     PASVGLSVLALLFFIMTRQPYVSAFSLCLLLLKGILLIKRR"
     gene            complement(11652..12170)
                     /locus_tag="PLQ29_00290"
     CDS             complement(11652..12170)
                     /locus_tag="PLQ29_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85110.1"
                     /translation="MRILSVTLMASSLILVPTLQAQEADGGAEYSIETNLAYIALGAV
                     SCTGGNVFIVAPLYSQLRVFGRFSVDLASAYIYNSHPTLATPSSMILAEAGASYHPSG
                     AIRGWTFGLLPGVTYSFDSRIAGFSISANCGYKWVIGKGLVLGALTGVRYVWIDGYIV
                     LPDLAINLGYRL"
     gene            12350..>13743
                     /locus_tag="PLQ29_00295"
     CDS             12350..>13743
                     /locus_tag="PLQ29_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG85111.1"
                     /translation="MLRTIRARLMAINLLLALLIGSLFAANYLSLAGLGALQRDGRTR
                     SMDAKAVAEASYVGSDLFAVIATLIINRDLAEFGAAWPAAKARASERMDAALAVAASD
                     EDRTLANRARGALDELIAAVEGALAPLAAGTDADRTRISPSMRVLGYQIRLLVDQVER
                     DLKAVSESVEREAVLADEAFAALSARTAATGLALGLVAIALAVGTMMANLRLVLRPLR
                     SQLGLLDDMAGAEADLTRRLDQARDDEYGRLGRSFNAFMERLVASVDAVKSAAAALAG
                     QTAALAERMEDTAAAVEQIGANVESIRKRSEAQALAAGRSAESLERVTTGLTELDALI
                     ASQAASVAESSAAIEQMVANMRSVAGAVESNSASIEELQRSAIDGKEWMDEVASLLRG
                     MLSDSAVLSEAGAVIQGISSQTNLLAMNAAIEAAHAGEAGKGFAVVAEEIRTLSDMAA
                     EGSGSIAAILAETR"
BASE COUNT         2391 a         4673 c         4547 g         2132 t
ORIGIN      
        1 cgagctcgat cacgatcacc ttcgattggg agatcgatgg cgacgcggcc agggaccagg
       61 tcgtcgccgc gatgaacggc gtcaggagct acctgcccga ggacctacgc gacgcctact
      121 cgatacgctt ccgcgagggc gagaacgccg gcttcatcgt gatgggcgtc gtgtcgaacg
      181 cgacgccgcc cgaggagctg atgccaatgc tccaggccgg cgtcgagagc cgcctggcga
      241 cgatagacgg cgtcgacgag tacggcatct atggcctcga ggagctgagc gtcgaggtga
      301 ccctcgacca cgaggcgatg ctggccgccg gcgtttcgat gctcgacgtg gacgcggcct
      361 tcagggccgg cctgtcccca tcgccgctcg gcaccctgcg ggagggcgac gaacgctata
      421 gcctgcgctt acggaggccg acggccgggc tcgagtcgct aggccgcctc gagatagccc
      481 gagacggcct ggcggtcacg cggctcgagg atatcgccga gatcgacgtg cgctacgacc
      541 taccgtcaag ggtcttcctg ctcggcgaca agccggccat ccagatcacg ctgacgcccg
      601 aggacggcgg caacctgaac cgcatgaccg aggaactagt ggtaataatg gaagaagcca
      661 aggcttccgg ccgcctcccg gccgacgtcg gcttcgagct gtacctggac ccggccaggt
      721 acattaaccg ctccattagc aacgtcgtcc agtcggctct gctcggcggc ctcctggccg
      781 tcctgatcgt tttcctgatc ctgggagagc tgcggaacac gctgatcatc gcgctgacgg
      841 tgccggttac catcgtgctc tccttcatcc tgatgtccct gttcggcctg accatcaacc
      901 tcatatcgct cggcggcctg gcgctggccg tcggcatgat cgtcgacccg accatcgtgg
      961 tcatggagaa tatccaccgc tggcgcgccg aggcgaggcg cgccggtgac gcgagcggtt
     1021 cggcggcggg caaggacctg gtagccgacg ccaccaggca ggtacgcggc cccgtcatag
     1081 cgtcggccct gacctcggtg gcggtcttcc tcccgctcac cttcaccgcg ccgctcgcgg
     1141 cggccatcct cggcgaccag gccaagaccg tggtgttctc gctactcgtc tcgctactgg
     1201 tctcgctaag cttcgtgccg gtagccgcgt acctactctt cggtacgggg aaacgcgcgg
     1261 cgctctccga gccgcgcggc tacgcgaaga tagccgatgc cgccatggat cgactcgtgg
     1321 aggcctatcg acgcgcgctt cgcgccgtga tacgaaccag gccgagggcg gccaccttcc
     1381 tgactctagc cttcgcggcg ctcgtggtat cggtagggct cctcttgccg cgcgtgccca
     1441 aggagatgct gccgacgccg agctccgacc gcgtagttgt cttcttccgg cacggcgact
     1501 acacgacggc acccgaggtg atagagcggc taatgccgga actgcgcgaa cggatagccg
     1561 gcgcgatgga cccgatagcg tataaagact tcgccagcgt ctccggtcga atgaatcaga
     1621 tcttcgtcga cctcgagagc acggaatata ccgacgaggc gatcgtcagg ctcgaggcgg
     1681 aattcgagtc cgagggccag tggtacttca gcatagcctc gtgggacccc gccgccctgc
     1741 ccctgccgca gacgaacgac ctcaggctca gcgtccacgg cccggacccc acggtcaagg
     1801 tcgccctcct cgacgagatg caacgagcgc tcaacgactc gaggctgtac ggccggacct
     1861 acgcgagacc ctccccggct cttacgcggg aggtcgcgct cgagacgagg ccgggcgcgt
     1921 tcgacggttt cccgggtctc ggcgaggctt ccatggccac gctcgtcaga aggtcgctgg
     1981 ccggcagcgc cggcctgtcg ctgagcgacg gctcgagcga ggtatcagtc tcggcgacct
     2041 atcccgacgg cctcctcgac tcacgcgacg agctggcccg cttcctcgta ccgtggaagg
     2101 gcacgggcgt cccgctcaag cacttcttcg acttcgagga atccgagacc gtgtcgcagg
     2161 tctactccga gaacggcgag gccgcctaca ggctctacgc caacatctcg ggcgaggcga
     2221 gcgacgcggt gcggctcgag aaggaagcgg cggcccgccg gctgttcgac tcggccatcg
     2281 agccgccggc cggctacggc tattccttcg acaacccgcg aggggagctc gacgaggcca
     2341 tctcgtcact cctggccgcc ctggcgatat ccatcgggct cgtgtacctg ctcctggcct
     2401 tccagtacga ctcgttcggc gtgccgttgg ctatcctcgt agccgtgccg ttcggcttcg
     2461 tcggcgtggc gctgtccttg ttcgcgttcc gctcgacgct caacctcaat tcgctgctcg
     2521 gcaccatcct gctctgcggc gtcgtcgtca ataactcgat catcatgatc gacttctacc
     2581 tgaagacgcg caagaaccat caggacaacg cgagcgccat cgaggcggct gcggccctac
     2641 ggctcaagcc gatactgatc acgacgctga cgacggtatt cgggatgctg ccgatagcgc
     2701 tcggcatggg ctcagggtcg aacatcctgc agccactcgg catagcggtc tcgggcgggc
     2761 tggcgatctc tacgctcctg acgctattcg ccgtgccggc gctgctcagg ttctggaggt
     2821 tccccgccta ggccaggcgg ctacttcttg ccgtagaccg cctgaggctg ggtcttgccg
     2881 cggaacagga cctcgcccat cggctggaag cgggactgcg gctcgagcga caactgcttg
     2941 tagaccggct ggctgatgag caggtcctgc ttgaactcgc ggcacagcga gtcgagccgg
     3001 gccgcgatgt tgaccgcctc gccgaccacg ccgacgcgct gcacgccgct cgccgacgat
     3061 agctcgccga cggccacctt gccggcgtgc acgcctatgc cgatgtgctt gaccaggggc
     3121 aggcgatggg ccgccaggtc ctcctgcagg tgtgaaaact catacaggaa gtcgaacgcg
     3181 cagctgacgg cctggtcggc ggcgtccttc tcgcccatca ggccgaacac gacgatggcc
     3241 gtgtcgccga cgaattgctc gacgacgccg ccgtgcttgc gcgcgacaat gtcccagagc
     3301 gcgtagtagc ggttgaggag gcctatcgtc gccctggcgc cctccttctc ggctatcggg
     3361 gtaaaattcc agaggtcggc gtacagtatg accacctcgc gattctcgcc gtcggacacc
     3421 tggtcgaaca tcgacggctc ggccgaaggc ggctccacga tcgagtacac ctggtcaagc
     3481 ctggccttca tcgacacctg cttgatcttg tcggccacct ggaaggagcc gaacataacc
     3541 gcgccgaaca tgaagaagac gtgctgcagg gtcttggcga actcgccgaa ggccggcccg
     3601 aagttcgaaa gtttgaggct cgagaccaac gctgcgtagc cgagttcgcc ggcctcgtag
     3661 gccttggtgc tggtcaggcg gaggtccagg tagaaataga agaagatgaa cgacaggata
     3721 gtgccgagcg ataggaatat ctcggcgata cgcttgggcc acttgcgccg gaacaggatg
     3781 gccagcgcct ggatgccgcc caggtataga gaggtagccc agaggaagaa gctggccata
     3841 tcgatgccct gggccgactt cgacagcgtc aggatggtcg tatagccgag cggcgtcatc
     3901 agcgagaaga agaacctggc cggcggccgg tgaccgaagc gcgccaggag cgctatctga
     3961 aggagcagga acacgatcag cgatatgagc gccgaggtgc tcggcctgat gatggccggc
     4021 gaggtgaaca ggatgatgat gaagaagagt acgaattggc tcgaatagag gaagaactct
     4081 tctaggtaac gcttcggtag gaatctcatg tagtcgacca tcatacccga taggggcaag
     4141 cctgtcactc cttggaagac gtagggcacg gtggcgcgct atgggagtat actgcatgac
     4201 catgactacc tcgcggcttc ttcatgggag aacgcgtagc ggcgcaccca acatcagggt
     4261 agctcggctc gcgatccatg cagcggtact ggctgtaacg atgacggcgc tatcgacgat
     4321 agtggcggcc tgctcaagaa gcgcggagac gacggacgct gacggcccgt cgctcccagg
     4381 cggcatcgat gggcgagccg ccttctccgc gatcgaggcg ttgaccggcc cgttatacgc
     4441 cggcaggatt gtcggcggcg atggctccgc cgcggcccga gcctacctcc gggcgaggct
     4501 cgaggctgtc gggctggccg tcgaggggct cgaattcata gaacgcgtcg cgctcaacga
     4561 cggcgaggcg ttcctgcgag cgaggactcc gggcggggcg acgacggtgt tcgagtaccg
     4621 atccgatttt agggaggctc cgatcaatgg cttcgacggg ggccgggcct cgggtccgct
     4681 caagcgactc accggctcgg gcttcgagcc gggcaccatc ctgcttgcgc ctaaggaggc
     4741 gcgccccagc gaaatcgccg ccggcctcgc ggcggccggc gccgcgggac tgctcttcga
     4801 gttggacaag tcgtcgcctg cactgcggcc gctatggccc ggccacgagc cgggcgccct
     4861 cgtgaaggtc aagcacggca tgcccatact cggtctatcg ggcgaggcct tcgccgctct
     4921 cgccgcgatg gtcgcgacag ccggcgccga tcccggagcc gacgccccgt tcatcgagct
     4981 ggccagccca gttcgcttcg ccgacgttat cggcgcgaac ctcctggccg tgtggaacgg
     5041 cgacggaggg tccttcgagc ccgcctacct ggtcatggcc cactacgacc atgtcggcgc
     5101 ggatcccgac gggagctact tccccggcgc cctcgacaac gcctccggag tcggcgttgc
     5161 gctcgcgctc gccgaggccg ccgcctcgta ccggcctcgg gtcgacctgg cgttcctgtt
     5221 caccgacgcc gaggagggcg ggctcggcgg gtcgacggcc ttcgtatcgg ctccgccgtt
     5281 cccgctcggc ggagcgtcgg taatcaacct cgacatgcta ggctcatgcg ccgaaacccg
     5341 gtacagcgtc tactcgagcg gcggccaagc cggcgtctcc ctgtcgctac gagtggagcg
     5401 ggccctgagc tcgggcggca tcccggctac atcggagcac cctgtcgacg tttcggacca
     5461 cgcgccgttc gcgagcgccg gcgtcgctgc ggtgaccgtc tgcgaatacg acaccgagca
     5521 ttaccaccgc aaggacgacc tcccggagta cctggatacg gacgagctcg acgccgtcgg
     5581 cgacgccctc taccgcctca tcaccacggc ggcggtcgca gggccgtaga ggttcgggaa
     5641 gaacgagtcg atgacgccgt aggaggcgaa gccagcggct tcgtagatcc ggcgcgctcc
     5701 cgtccaggcc tcgttgacta tcagcacctc ggtttcgatg cccgaggcgg cggctcggat
     5761 catcgcgata ccgtcattga acagcctgga accgagtccc gtgccgcgta gcgcggggtc
     5821 gatggtcatc gacgcgaggt atagtaccga tccatccggg tcatgtcgcg acgctggatc
     5881 gtggccgagc tcgaaccacc gggcctcggc gggcggcacc tctgcccagc gttcagcgca
     5941 caggtagcct acagtctcgc cgcctcttcg cagcgctacg aaaccctcgg gaaagagctc
     6001 catccgcgca cggaacacct ccgcgtgttc gcggatgccc ataccgaagc cggcgtcctc
     6061 gagccgcatc accgccggca ggtcgtctag ccctgcgcgc ttcatgacca cccgggcgga
     6121 agcccgccga ccgcgcgctc tacccgcgaa ggcgccggcg acgaggaggc acggcgcccc
     6181 gacgaaggcc agccccgcga acgcccactt gagggtagtc gcgacgctcg cgcagacagc
     6241 ggcgatgcca ggagtagccg gagcgctttg cctggccccg acgagcagcg cggccacggc
     6301 cacgttctcg gctagatcga acgccgttga ggccagcggc acgagcagga agccgtaacg
     6361 aggctcgctc gctggtcggg ccagtcgccg caatccgaag gcccaggccg aggcgcagaa
     6421 cagtccatag gcgagcggga acaccagatc gagcgtccaa tggacgcgaa tcgacgcgcg
     6481 gacctccgcc tccgtatacg acgacgcctt cctgaccgcc tcggccggcg tgtagaagag
     6541 gctcaggtcg aaggcgccgc cgtcaggcgt agcggccgcg agcgacgagc cgagcgccgg
     6601 atagccgata accatgaata ccgcgagcag cgcggcgctg gtcgcgagga cgtagccgcg
     6661 cgatagcatg acagccagtc gtttcatcgc cccagtataa ccccgccgta agccggccga
     6721 tagccggcgc gcggcccagg gccggcgcgg ccgcccggcc gcggccgcgg cgcagtccgc
     6781 ggctggggga ccggtctatc gtcgtcctcc acgttttaaa aggcgatacg ccgtttcaac
     6841 ggaccgtact ttcctcgcga cggcctcgac tgctatactg agaggagccg cccgcgaggc
     6901 ggcgcgtaag gggggaaccg atgtgcggaa tagtctcgct cgtctataag gacgagacgc
     6961 ggaacatggg ccgtgaggcg gcggagctgt tgcgtaggct cgagtaccgc ggctacgact
     7021 cgacgggcgc ctcgttcata ggcaaggacc gcaacatcgt gctcgtcaag cgcgtcggct
     7081 cgccgaccag ggtctgcgcc gagctgaacg tgccgtcgta caagggccag cgcttcatcg
     7141 ggcaggtccg atgggccacc tacggcgcgg tctccgacgt gaacagccag ccccaccacg
     7201 tgcgctgcaa ggtggagatg gtcggcgccc ataacggtaa catctcgaac accgactcgc
     7261 tccaggccag cctgagcgcc cgcggccata aggtcgtgtc tgacaacgac ggcgagatca
     7321 tcgtccacct ggtcgaggac cactacgccg ccaacaaggc cgcgccgcag aaggccctgg
     7381 ccgagatgcg ccgggcctac gccgcgtccg gcctgagcgc gccggtcgcg gacgaggtgc
     7441 tcctgatcgt cgacgcgata cgcaaggccg aggccgaggc cgacggctcg tacgcggccg
     7501 ccgtcgccga cccccaggtg cccggctgct tcgcgatcaa gtccggcccg tcgctgtacg
     7561 ccggcctggg caaggacgag gacggctcct tcgtcgtcgt atcgagcgac ctgacctccg
     7621 tgatagccaa gaccccgatg ctcataccgc tcgccgaggg cgagggcctc tggtatacgg
     7681 agggtcgcta cctggtgttc agcctcggcg gcgaggcccg tttcgccgag cctaggctca
     7741 agcgctcgaa gctcaacgta cgcgacacgg gcctgaaccc tcgctataaa tactacatgg
     7801 agcaggagat agcctccgcc ccggacaaca tggacgcgat cgagcgctat tatttcgccg
     7861 acgaacgcga agccggactg gcctccgtac tcgaggacaa ccgctcggtg gccaaggagc
     7921 tcatggacaa ggcctgcgac ctggcctcgg cggccaacga cgcggcgctc ggcgcggcct
     7981 tcgacgagct cgccgcctcc tcggattggc gcgcgctcgc cgccaaggtc aatgccgacg
     8041 cggcggcccg cgcgccccta gcgggccgga cctccttccg gtccgacgaa gcccagctgc
     8101 tcaccgatct ggcccgtctc cggccggccg ccgccgccga cctggccctg ctcgacctgg
     8161 cgatgatctg gcgcaagcgc cgttccgtga cgcgctacgc ccgcgagctg tcggaccgca
     8221 tccgggagac ccgcaaggcc ggcggcaccg tctacgtcgt cgcctccggc acctcgtacc
     8281 acgcctccct gaccgccgcc tacttctgga acggcctcgc cggcgtaccg ctctacccct
     8341 gcaacccggg ccagttccgc tcggcctaca tggactcgct cggcgacgcc gacctgatcc
     8401 tggccgtctc gcagtcaggc gagaccaagg acctcgtcga catcctgcag gacgtcgccg
     8461 agacccgccc gaggatcaag cgggccgccc tcgtcaacaa cgagaacagc cgcatccccc
     8521 aggagctggc cgacttctac ctgccgatac tctgcggccc ggagatagcc gtcgcggcga
     8581 ccaagagttt cgtcaaccag ctcgtgatca tgtacatcct ggccgcctcg tataagcagc
     8641 ccgaggccgc cgtcaaggcg tccgtgaacc gcgcgaaggg actgctctcc gacaccctga
     8701 ggctatccgc gccggccatc gacgaggtgg ccgagcggct gtacctgaag ccgtcgatcc
     8761 acatgctcgg taccgggctc ttcggcctgg cccgcgaggc ggccctcaag gtgcgcgagg
     8821 tggtcctgaa ccactcggaa ggctacgacg cggccgagtt caagcacggg ccgaacacca
     8881 tcctgggcaa gaacaccgtc ttctcgatag cggaggtcgt caaggcgatg gacgcagcgc
     8941 tcgcggccgg aaccgccgga acaggcgccg gaacgggagc cggcaagagc gccgcggagc
     9001 tgctcgccgc cgatccgtcc atcgtcgagg ggctgttctc caactacccc ctcgtcttcg
     9061 tctgcccccc ggacgcccgc gacgcgcgca tcaccatcag ccagatccac acccataaga
     9121 tccgcggcgc cgacatcgtg ctgttcgcgg agaagcatcc cgagctcgac ctggccgtca
     9181 agggcgttcc ctccggccgc gacgactacc gctcggccta cgtggagata cccgcctcgg
     9241 gcgaccggta tctcttcgtg ttcgccgcca ccgtcgccct ccagtacctg gccttcagga
     9301 tgtcggtgct caagatggag tacctcgacc gcctcggcgt acgggaccac ggcgtccacc
     9361 cggacgcgcc caagaacgtg tccaaatcca tcaccgtcga ttaagcgcgc gaggtcgacg
     9421 atacacctcg catacggccg ggccctttcg ggcccggctt ctgtttgcgt agcgcggcgc
     9481 tggctatcga gtgggcgccc gggacgggct cgcgggggcc ggaggccgga gcggcggctc
     9541 cgagccggcg tgaaacgagg cgacaggcgg gctatagcgg ccgtcaggac gggcctgagc
     9601 gccgcgaccg cgggtttcag gaacctgaag ttagccgtga acacctcgtt ccactgatcg
     9661 aaggtatggt tctggtagcc gaggatggac tggacttccg cggtgtcgta ccagtccagg
     9721 taatacgatc ccaggttgtc ggggaagagc tcctcgcgcg gcatggcgag ccccatagat
     9781 ccgaaggctc cttcgagcat ggcctggatg cgtatctggc atccgttcgc ggcgccgccg
     9841 gctatgaagc cggtcctacc ccgcatctct cccgcgccgc ggaggttctc gaccgccgag
     9901 accagggtgg aagccgcgtc ggcgtcgtag acgatctcgc agcgcgccgc gagcggcatg
     9961 tcgtagataa tccgtatcat gcccaggacg gtgcccaggc cgacgtagct cggcatcacc
    10021 gccgcggcct tgatcctcgt gtacgcgtcg accgggttgg ccgggtcgcc gatacggacc
    10081 cggacgccga cgcggccgta ccgccgcccc agccgctccg cgcgcggcca ggcactcgtc
    10141 ggtgacgcgc atgccgacat tgccgaatcc gcccgtcacg agtatgcgca tggtcggctc
    10201 tccttatcgg ccggcctgtg gcgcgccggc gcgcttcgat tccccctaca ttctaggata
    10261 ccggtcggcc ccgcgccagg gcggcccggt acgacgcggc cagcgccgag ccaaggagca
    10321 gtccgccgat gatatccgac agccagtggg cgccggagat cagccgcccg agcgcggtaa
    10381 cggcgatgac ggcggccgac gccagttcga gcgccgcgag caggcggcgg ctccgggtcc
    10441 gcctgcggaa ctgcagcacg gccgtcgcca tgacgcctac ggccaccatg gtatggctcg
    10501 acgggtacga cgcctctagc cggccgccga tcaggatagg cctgtaattg acgacgaggc
    10561 gctcgaacag gacgtagtag ccgacgaccg ccaggtacaa cgccccgagc agcatgatat
    10621 ccgcgtcgac gcgccgcagg ctccggcgcc ggaccagctg ccatgccccg aggcaggcga
    10681 agccgcaggc cacggctacc ggcaccaggc ccagccagtc ggtcagggcg taccagaacg
    10741 gctgcgcttc gagcagctcg tagattaatc ggttcatggc cgcgaggccg acgaccgacc
    10801 cgtccggtcc tatcgcctcc cggtcgaccg tgccgaccgc gacggtgaat agtatgaacg
    10861 ccagccaaag gccgaccggc gcggccatct gtgccgacga ccgaggcacc atcggagcca
    10921 cggtcgggac cgagtcattc gttcgcttga tcatgattga tcctccctcc gcgcattagg
    10981 ccgcggacgg gagggtatcg gccagaaacg aagccttaca tcggagatac ttttagtatc
    11041 agcggcgctt gatgagcaga atccccttga gtaggagcag gcagagcgag aaggcgctga
    11101 cgtagggctg gcgggtcatg atgaagaaca gcagcgccag gaccgacagc ccgaccgaag
    11161 cgggcaggct caggcggcgc caggccgggc tccgcaggtt ctgcagggcg agttgcagca
    11221 caccgaaggt cgcggtggcg accacggcga cgtagtacgc cgccacgata tcaggcggca
    11281 tgccggtcac ccggggcaag gccacggacg cgatccgccc gccgatctcg tctccgaata
    11341 gcggcagggc gaggagcagc gtcatcatca cgtccagcac gccgaaggcc acggcgagga
    11401 ggccgcccga cgactccctg gactggctct cggcgacgga caggagttcg tcgccggaca
    11461 gcaggtcgtc gatcgacacc gacagggtct tggccatcag cctgagcgat tcgatattcg
    11521 gatagccgcg ccccgactcc catttggaga ccgtgaccct cgacacgcac agttcgtcgg
    11581 cgagccgttc ctgggtcagg ccggccctgg ccctcaggct ccgcagtttc tcgttgaatt
    11641 ccattcgccg tttatagcct gtatccaagg ttgatcgcca ggtcaggcag gacgatgtac
    11701 ccgtcgatcc atacgtaccg gaccccagtc agcgccccga gaaccaggcc cttgcctatc
    11761 acccacttgt acccgcagtt cgccgagatc gagaacccgg cgatacgcga gtcgaacgag
    11821 taggtgacgc ccggcagcag cccgaaggtc cacccgcgga tcgccccgga cgggtggtac
    11881 gacgcgccgg cctcggcgag gatcatcgag ctcggcgtcg cgagggtcgg gtggctgttg
    11941 tagatatagg cggaggccag atcgacgctg aagcgcccga agacccgcaa ctgggagtac
    12001 aggggcgcga cgatgaagac gttgcctccc gtgcacgaga ccgccccgag cgctatatac
    12061 gccaggttcg tctcgatcga gtactcggcg ccgccgtcag cctcctgggc ctgaagcgtc
    12121 gggacgagga tcaaggacga ggccatcagc gttacggaga gaatacgcat cgtatacctc
    12181 ctagagctag tatccggagc ccgctgtccg ttgcgcgcac cgacgacgcg ccaaagcggg
    12241 gttgtcacgt acagtatagc cgcggacgcg agctaataca ccccgcacag tttgacggta
    12301 cgcagattcc ggtacatact ggccgttccg cgctttggag gtgatacggg tgttacgtac
    12361 gatcagggcc aggctcatgg cgatcaacct gctgctcgcg ctgcttatcg gctcgctatt
    12421 cgccgccaac tacctgtctc tggccggcct cggagcgctt cagcgggacg gcaggacgcg
    12481 ctcgatggac gccaaggccg ttgcggaggc atcctacgta gggagcgacc tgttcgcggt
    12541 catcgccacg cttatcatta acagggacct ggcggaattc ggcgccgcgt ggccggcggc
    12601 caaggcgagg gcctccgagc ggatggacgc cgccctcgcc gtcgcggcca gcgacgaaga
    12661 ccgtacgcta gcgaatcggg cccgcggcgc gctggacgag ctgatcgccg cggtggaggg
    12721 agcgctggcg ccgctggccg ccgggaccga cgcggaccgc accaggataa gcccgtcgat
    12781 gcgcgtgctc ggctaccaga tacggctgct cgtcgaccag gtcgaacggg acctgaaggc
    12841 ggtcagcgag tcggtggaac gagaagccgt gctcgcggac gaagccttcg ccgccctgtc
    12901 agccaggacg gcggcgaccg gcttggcgct cgggctggtc gccatagcgc tggccgtcgg
    12961 gaccatgatg gcgaacctac ggctcgtgct gcgcccgctc cgctcgcagc tgggcctcct
    13021 cgatgacatg gccggggccg aggccgacct gacgcgaagg ctggatcagg ccagggacga
    13081 cgagtacggt aggctcggcc gttccttcaa cgcgttcatg gagcgcctgg tcgcttcggt
    13141 cgacgcggtc aagtccgccg cggcggcgct ggccggccag acggccgccc tggccgagcg
    13201 catggaggat accgcggcgg cggtcgagca gatcggcgcc aacgtggaga gcatccgtaa
    13261 acggtcggaa gcccaggccc tcgccgcggg gcggagcgcc gagtcgctag aacgggtcac
    13321 gaccggcctg accgaattgg acgcgctcat cgcgagccaa gcggccagcg tcgccgagtc
    13381 gtcggccgct atcgagcaga tggtggccaa tatgcggtcg gtagccggcg cggtcgagtc
    13441 gaactccgcg tccatcgaag agctccaacg gtcggccatc gacggcaagg aatggatgga
    13501 cgaggtggct tcgctcctgc gcggcatgct gagcgactcg gccgtcttgt ccgaagcggg
    13561 cgccgtaatc cagggcatct cgagccagac caacctgctg gcgatgaatg cggccataga
    13621 ggcggcccac gccggcgagg cgggcaaggg cttcgccgtg gtcgcggagg agatacgtac
    13681 gctatcggac atggcggccg agggctcggg gtccatagcc gcgatcctcg ccgagacgag
    13741 gga
//
LOCUS       DAOPHV010000005        13163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_5528_length_13163_cov_3.933933, whole genome shotgun sequence.
ACCESSION   DAOPHV010000005 DAOPHV010000000
VERSION     DAOPHV010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5528_length_13163_cov_3.933933"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(594..812)
                     /gene="csrA"
                     /locus_tag="PLQ29_00300"
     CDS             complement(594..812)
                     /gene="csrA"
                     /locus_tag="PLQ29_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HPG85112.1"
                     /translation="MLILSRKLNEKVVIGDGVVVSIVEIRGDQVKLGIEAPRSVKVFR
                     QEVYDAIQDENRLAAGSAAELPALELPQ"
     gene            complement(817..1257)
                     /locus_tag="PLQ29_00305"
     CDS             complement(817..1257)
                     /locus_tag="PLQ29_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HPG85113.1"
                     /translation="MKIQTKILGELDVDERQVIDIPDGLIGFKKFTKYALLDAPQKPY
                     FFLQSTELAELCFILLDPFLFRPDYSVDAADEAMSALGVTGPDDALVLSLVTVPPDGG
                     PVTANLMGPLVIGRKSRRGAQVVLADPRWRTKHDVMAEMAASRK"
     gene            complement(1280..2521)
                     /locus_tag="PLQ29_00310"
     CDS             complement(1280..2521)
                     /locus_tag="PLQ29_00310"
                     /inference="COORDINATES: protein motif:HMM:NF005187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HPG85114.1"
                     /translation="MRRVSSEFMNTDMQYWLRRNEDRMASMQSKLSRQERLESPRDDP
                     MAAARAVRYESFVGRLSRYEENVLYAKDRADIAEGYMRQGMDIMQRVRELAITGATGT
                     FTKEDSQAMAVEVDQLLAELVSLGNARGPDGDFLFAGDKAKTEPFRALQGYAEGAGAA
                     TTVGVDYLGGLGTPKAEIAEGSYLPLNLAGDSVFWSERQRIASAADASAYRVMDSASL
                     FVDGHEVALRPGDTVQAIIAKINGSGAAVKASLDPAKNALVLEATDAHRVRLEDGAGS
                     SVLADLGVLSGSRVPSDYAPTARVSGGSLFDSVIALRDALNKGDFIDVGGRVLASLDA
                     GMENLGRRLAEAGSLSERLDMAALRLNREIPDVTKLLAAEKDLDMTQAITDFKMMEYA
                     RNATLQMAGRVLPKSLLDFLR"
     gene            complement(2711..4585)
                     /gene="flgK"
                     /locus_tag="PLQ29_00315"
     CDS             complement(2711..4585)
                     /gene="flgK"
                     /locus_tag="PLQ29_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HPG85115.1"
                     /translation="MQSTFAGIELGKRGLVAHNQGLQTIGHNLTNAATEGYSRQRVEL
                     GTMEPLYAPHLNREETPGQVGQGVVAVRVERMRDELLDRRIVAQTGGLGYWDTRDKYM
                     RLLEQIHNEPTEVSTRTQMDRFWDAWQELSLHPSELAPREAVVQRGKTLIDSIHGQYQ
                     RLSATRDMVQDDIEISAAQANDLSRQIAALNAEIIKSEAMGDNPNDLYDRRDVLVEKL
                     ATIVPVSVDRRDPDEFMVHVDGHILVQGKIARGFELSTGAESGGYPRVLWADTGDEAV
                     IPGGSLGAMLEMRDVDLKEEIASLDTMTLTFTDLVNEVHRSAYGLNGKTGVDFFTELP
                     SVNNIAGNYDRDGDGAYDHSYVYRLSGGNALDAQEQLGLAGTMRLSGPRGLVDVEYRP
                     TDTVGDLVRRINDSGADVTARLTSTGALQLRAKTASDGESPDFVIRHVEDSGEFLVGY
                     AGLLAASGPDAAYSWDRADAVTGLRGGVDYAVAPLAHPSGWIEVSSAVTRDLTSVAAG
                     FGSNGRPAEAGDGSAAIAIARLRVAPVMVGSQKTFDDYFADAVASVGLKGERAGRMAD
                     TQSRVVKNLTDTRDSISGVNMDEELSNMIKFQHGYSAAAKFVSTMDSMLDTIINRLRG
                     "
     gene            complement(4600..5082)
                     /locus_tag="PLQ29_00320"
     CDS             complement(4600..5082)
                     /locus_tag="PLQ29_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85116.1"
                     /translation="MMTFIARVGEQIEALDAFVEDQRAVAGAVRARDWPALERALERA
                     GASAGRVSSAEALRVAAWESLVFGLGLPADSSVFRASLLLPLEFRSTLNESYRSLRLS
                     AMRARIENDALSGFVGSAASTLREAMEAAFPERKGRIYGRTGLPRHAGSGAMILDTAL
                     "
     gene            5227..6093
                     /locus_tag="PLQ29_00325"
     CDS             5227..6093
                     /locus_tag="PLQ29_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85117.1"
                     /translation="MFKALTNLYWLARGVRVFALVGGSGTGKSFRAKLVAQKFGIDMI
                     IDDGLLISGDAIVAGKSAKREKLYMAAVKTALFHEKAHRDEVARVLQKTRWKKILIIG
                     TSEKMTMKIAERLQIPYPAKTIKIEDIASREEIDTAIRARKVEGKHVIPVPAIEIKRS
                     YPRIFYDSIRVFLQRNLSPGGLTTPKLYEKSVVRPEFSKRGRVAISESALSQMVIHCI
                     DEFDDAIRIQKITVRSDSQGYRLTVVIEVPFGSQLSGKIHNLQQYIIDNIERFTGILI
                     EEVNLVIDRFSK"
     gene            6162..7445
                     /locus_tag="PLQ29_00330"
     CDS             6162..7445
                     /locus_tag="PLQ29_00330"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG85118.1"
                     /translation="MKKILSFAVIALALAVCAGAQDAAGVTNPAPAAAPVAAPAATAE
                     VVAVPAAQPTAFSSASSKRYAVWSDAGKDDAVRLATTLDGLFAVYNEYFRFDESALKA
                     PLTVRKFAEKASFDAYLAKVIGETKDDFVYLHYPTIERSELVIFGKASADDFDASLAH
                     QAFVQFLKAFVPEPPLWIREGFAVFFERTRANPSTGVVAYAENQSWLETIKLLKSQDR
                     LYTVSELMLLSSDEAKSGLDVFYPQSWALVSFFINSPDRRYNRFVWDAIGLLARDATL
                     EQNQNAVRDFFLRWHGQEAAESDFLTYLDGQKTFAELVTSGVRSYGDKDLETAYKAFS
                     QALTKNAASYIPHYYLGLIAYARGDYALAQDYYLKALELGSDAPITNYALGINAYASR
                     RFDEARAYLQKASDGDPTKYQEKAGEIITRISSGM"
     gene            complement(7543..8340)
                     /locus_tag="PLQ29_00335"
     CDS             complement(7543..8340)
                     /locus_tag="PLQ29_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPG85119.1"
                     /translation="MRIRFLGTGTSHGVPVLGCSCPVCSSTDPRDTRYRSSALVTGDG
                     GERILLDAGPEFRLQALAAHLDALDALLVTHAHADHVHGIDDVRPLTRGRPLPVYASG
                     LDASELRHRFSYVFSAGQAGGGKPRLELLDLDGPDGTGSAPRQVSVGSLVVRPVPLSH
                     GGRRVLGYRLDGLAYATDCSSVPDSSKAMLRGLDLLVLGALRLRPHPTHLSVDEALAL
                     ALELRPARLLLTHICHDLSHERLQSYCDDAGLPFPAGPAYDGLELEV"
     gene            complement(8337..9734)
                     /locus_tag="PLQ29_00340"
     CDS             complement(8337..9734)
                     /locus_tag="PLQ29_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85120.1"
                     /translation="MAPLKHDSRPCCRALVAALTMLAALSSVAPARADGAAGSLGGDR
                     PISSMPENDDIRIGAWARLIEGPKAQALAYPGAAARNGWGPWSISVIDGGDAFYVIFA
                     PGSGGAKPGGGPLYAQGSWIIKRSAADGSVLQAKVFVRSDPGTFVRLYPYGSRSRIDV
                     VAYGGVLYHKAIIPLPFEELLRSPFSRIVGLTSDVIDWSRFSPDPALYRDLRGLMASV
                     RAGLPSLRYADDGAIDADGRAVLISTLADQAKPAGLNCSGFVKWLVDGMLHPITGNYL
                     AVGRLKERMLDRRGSSFTIGFEERYDPFFGLDWSRALARAAWSAFYPSLRDESPLAND
                     VAEAPFALRVSDADPVNGGSAYEPYSDNFDDAGLDVRGLKAVLFMLASSEPGRFYLAQ
                     FNARESAPPNLRRYFHIAALFPYFDEDGAFRVAVFESAAETSLDAVTRRGYEFVKLVR
                     MPAASRFEPQALDRP"
     gene            9873..11048
                     /locus_tag="PLQ29_00345"
     CDS             9873..11048
                     /locus_tag="PLQ29_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85121.1"
                     /translation="MHRSTLVSIAVFVSAALVSLGSSSQFSESIGAGPEAASASGQEA
                     AQAAAGSASTSASTSSTAMAMAMATRPATADASSARTAEPYRLFPALLGAVPEPSSEG
                     RVVYGPRPPMSAEDAEALRRSTELAAAEAAERTPVLAGNQVLAFYGKPGAPSMGILGE
                     YTKEQLAPLLDGYARLYDGVNGDLGVVPAFYIIYGTCWPAGEIGILKDSVVRSYIEFA
                     AERGWLVFLDHQLGKYGVEESVRSMLPYLSYPNVHLAIDPEWRTLKPMEEIGSITGAE
                     LNEAQRLIDAYLRDHGLPGIRMLVVHQFKPTMIRDSGSVKAGFDRVVLVHTADGFGSP
                     ALKRLTYASNARLSNMPVKGFKLFFESKVVGAGWDKPLMKPEEVLALDPMPLVIMYQ"
     gene            11120..>13163
                     /locus_tag="PLQ29_00350"
     CDS             11120..>13163
                     /locus_tag="PLQ29_00350"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPG85122.1"
                     /translation="MILRAKPLALIAIASLALSLAAAYDPPSGAETAASLASPALLAG
                     GYSAASTESPMADLANPAASAAQQRLTLDLSYGALVGLADDTGWGSALNLGIAVPRAY
                     GVWTGYLGLLSSPFGSMPLGTVVTGRVGLAKDLYPDLLLGLAVDTRLGSNGGFGWGLV
                     ADLGVIGLAGDVGALKNLVWGASLRNMGKAFEAPGAVGLSGGLASAYDAPFTPQVGAS
                     ADLIRADKAGLKLSANADLWLPTFQNAVFATGLRLGWRDRAFLRLGWDFNLREAIAGV
                     DQGLLPSFGLGASFAIDRESDDSLLSKAGLDRSEIRPSFSAAELYGGVWAFSGGANVP
                     VGVLDRVPPRITVSYPAVEHDAYYISPNSDGARDLLTLPLSITDQRYVQSFSLKVLDE
                     SGAVVKTMANKESRPENQDLKGLLGRLLYVKKGVDVPAELEWNGLTDSGAAAPDGRYF
                     VVIEAVDDNGNVASTERWPVVIDTVPPSVKASGPAGDALIFSPDGDGSKDEFAIAQEG
                     SVEDSWVAVVTDAAGATVRSWTTASAAPSALRWDGKNDSGKVVPDGVYSYRVAAVDRA
                     GNQGSARIDNIIVNTQQPPVGVSIDLAAFSPDGDGVKDSLTLTPNVPVKAGLASWRLA
                     VLDDKGVERWAASGVGADSIEPAYRFSGKDASGAALPEGTYRTRLSVSYVNGHGPVA"
BASE COUNT         2101 a         4469 c         4456 g         2137 t
ORIGIN      
        1 tcgtcgctgg gaacgatttc ccgtcgccga ggagccattc tcagtcgctc gggaacctcg
       61 cgatcgccga ctggtgcggc gatcgccgac cgtcgtttaa cctgactcgc tacgctcgca
      121 ggttaacgcc ggcgcctcgt ccgcatccgc cgactggtgc ggcgatcgcc gaccgtcgtt
      181 taacctgact cgctacgctc gcaggttaac gccggcgcct cgtccgcatc cgccgactga
      241 tacccactcc gctccccggc acgacgcgcc gccccgccga acgtagtacc atcgcccgat
      301 agccacctta tgcactgcac gacgcgccgc cccgagaacc ttgttcgtac aaaggcctcg
      361 catacacgcg gaggcgagtg tccaggagac ggcgcccctc gcgtagcgag gggatcgcct
      421 cctggacagg ccctgccgag atgatcgcga ccgccgactc atcgcggcca tcgccggccg
      481 tcgcttaacc tgactcgctt ccgctcgcag gttaacggcg gcgaggcccg tattcattct
      541 cgaacccggc ccggggcggc gcgtcgtgtg atactcgcgg cgcgccgggt tacttactgg
      601 ggaagctcca gggccggcag ctcggcggcg gagccggcgg ccaggcggtt ctcgtcctgg
      661 atggcgtcgt agacttcctg cctgaagact ttgacgctcc tcggggcctc gatgccgagc
      721 ttcacctggt cgccccggat ctcgacgatc gacacgacca cgccgtctcc gatcacgact
      781 ttctcgttga gcttgcgcga cagtatgagc atcgcgctac ttcctcgagg cggccatctc
      841 cgccatcacg tcgtgtttgg tgcgccagcg ggggtcggcg agcaccacct gggcgccgcg
      901 ccggctcttg cgcccgatga cgagggggcc catcaggttg gcggtgacgg ggccgccgtc
      961 cggcggcacc gtgacgaggg acagcacgag cgcgtcgtcg gggccggtca cgccgagcgc
     1021 cgacatcgcc tcgtccgccg cgtcgaccga gtaatcgggc ctgaacagga acgggtcgag
     1081 caggatgaag cacagctcgg ccagctcggt cgactgcagg aagaagtacg gcttctgggg
     1141 cgcgtcgagc agcgcgtact tggtgaattt cttgaagccg atgaggccgt cggggatgtc
     1201 tatcacctgt cgctcgtcga cgtccagctc gccgaggatc ttggtttgta tcttcatgat
     1261 gccgccaaat ctgccggatt catcgcagga agtcaaggag cgacttgggc agcacgcggc
     1321 cggccatctg gagcgtcgcg ttgcgggcgt attccatcat cttgaagtcg gtgatggcct
     1381 gggtcatgtc gaggtccttc tccgcggcca ggagcttggt cacgtcgggt atctcgcggt
     1441 tcagccgcag ggccgccatg tccaggcgct ccgacagcga gccggcctcg gccaggcgcc
     1501 tgccgaggtt ctccatgccg gcgtcgaggc tggcgagcac gcgcccgccg acgtcgatga
     1561 agtcgccctt gttgagcgcg tcgcgcaggg ctatcaccga gtcgaacagc gagccgcccg
     1621 agacgcgggc ggtcggcgcg tagtccgacg gcacgcgcga gcccgacagc acgccgaggt
     1681 cggcgagcac gctcgagccg gccccgtcct cgagccggac gcggtgggcg tcggtcgcct
     1741 cgagcacgag cgcgttcttg gccgggtcga gggacgcctt gacggccgcc ccggagccgt
     1801 tgatcttggc gatgatcgcc tggacggtgt cgccggggcg gagggccacc tcgtggccgt
     1861 ccacgaacag gctggcgctg tccatcaccc ggtaagccga ggcgtccgcc gccgacgcga
     1921 tgcgctggcg ctcggaccag aagaccgagt cgccggccag gttcaacggc aggtaggagc
     1981 cctcggctat ctccgccttc ggggtgccga ggccgccgag gtagtcgacg ccgaccgtgg
     2041 tcgcggctcc agcgccttcc gcgtagccct ggagcgccct gaacggctcg gtcttggcct
     2101 tgtcgccggc gaacaggaag tcgccgtcgg ggccgcgcgc gttgcccagg ctgacgagct
     2161 cggccaggag ctggtcgacc tcgaccgcca tcgcctggct gtcctccttg gtgaaggtac
     2221 cggtcgcgcc ggtgatggcc agctcgcgga cgcgctgcat gatgtccatg ccctggcgca
     2281 tgtagccctc ggcgatgtcg gcgcggtcct tggcgtacag gacgttctcc tcgtagcgcg
     2341 agagccgccc gacgaacgac tcgtagcgca cggcgcgggc ggcggccatg gggtcgtcgc
     2401 gcgggctctc gagccgttcc tggcgggaca gcttggactg catggacgcc atgcggtcct
     2461 cgttgcgcct gagccagtac tgcatgtcgg tattcatgaa ttccgagctt accctacgca
     2521 tctgaacccc ctgggactgc gatagatagg tatttgaacc acggagacac ggaggcgcgg
     2581 aggaagaaca taagggggat gaagatgaat agaagcatga aaactggaac ccttgattcc
     2641 actcctagtc atctctccct cctctccgtg tctccgtgcc tccgtggcca agacttttag
     2701 tacgcaaacg ctagccgcgc aggcggttga tgatggtgtc gagcatcgag tccatcgtcg
     2761 acacgaactt ggccgccgcg gagtagccgt gctggaactt gatcatattg gacagttcct
     2821 cgtccatgtt gacgccggag atggagtcgc gcgtatcggt caggttcttg acgacgcggg
     2881 actgcgtgtc ggccatgcgg cccgcgcgct cgcccttgag cccgaccgag gctacggcgt
     2941 cggcgaagta gtcgtcgaag gtcttctgcg agccgaccat gaccggcgcg acgcgcagcc
     3001 tggctatcgc gatggcggcg ctcccgtcgc cggcctcggc gggccggccg ttggagccga
     3061 agccggccgc gacgctcgtc aggtcgcggg taacggcgct cgacacctcg atccagccgg
     3121 acgggtgggc gagcggggcc accgcgtagt cgacgccgcc gcgcaggccg gtcaccgcgt
     3181 cggcgcggtc ccaggaatag gcggcgtcgg gtcccgaggc cgcgagcagg ccggcgtagc
     3241 cgacgaggaa ctcgccggag tcctcgacgt gccggatgac gaagtcgggg ctctcgccat
     3301 cggaggcggt cttggcgcgc agctgcaggg caccggtcga ggtcagccgg gccgtcacgt
     3361 cggcgcccga gtcgttgatg cggcggacca ggtcgccgac ggtgtcggtc ggtcggtact
     3421 ccacgtcgac gaggccccgg gggccggaca ggcgcatcgt gccggccagg ccgagctgct
     3481 cctgcgcgtc aagggcgttc ccgcctgaca ggcggtacac gtagctgtgg tcgtaggcgc
     3541 cgtcgccgtc gcggtcgtag ttgcccgcta tgttgttgac gctcggcagc tcggtgaaga
     3601 agtccacgcc ggtcttgccg ttgagcccgt aggcggagcg gtgcacctcg ttgacgaggt
     3661 cggtgaaggt cagcgtcatc gtgtcgagcg aggctatttc ttccttaaga tcgacgtcgc
     3721 gcatctcgag catggcgccg agcgagccgc cgggtatgac ggcctcgtcg ccggtgtcgg
     3781 cccagagcac gcgcgggtag ccgcccgact ccgcgccggt cgacagctcg aagccgcgcg
     3841 cgatcttgcc ctgcaccagg atatggccgt cgacgtgtac catgaactcg tccgggtcgc
     3901 ggcgatccac cgagaccggt acgatggtcg ccagcttctc gacgagcacg tcgcggcggt
     3961 cgtagaggtc gttcgggttg tcgcccatcg cctcgctctt gatgatctcg gcgttgagcg
     4021 cggctatctg cctggacagg tcgttggcct gggcggcgct gatctcgatg tcgtcctgga
     4081 ccatgtcgcg ggtggcggac aggcgctggt actggccatg gatcgagtcg atgagggtct
     4141 tgccgcgctg gacgacggcc tcgcgcgggg cgagctccga ggggtggagc gacagctcct
     4201 gccaggcgtc ccagaacctg tccatctggg tgcgcgtcga cacctcggtc ggctcgttgt
     4261 ggatctgctc gagcaggcgc atatacttgt cgcgggtgtc ccagtagccg agcccgccgg
     4321 tctgggcgac gatgcgccga tccaggagct cgtcccgcat gcgctcgacg cgcaccgcga
     4381 ccacgccctg gccgacctgg cccggagtct cctcgcggtt caggtggggc gcgtacagcg
     4441 gctccatcgt gccgagctcg acccgctggc gcgaatagcc ctcggtcgcg gcgttcgtca
     4501 ggttatggcc tatggtctgg agcccctggt tatgcgcgac gaggccgcgc ttgcctagtt
     4561 caatgccagc gaaagtcgat tgcacgatag cctcctaaat cagagagccg tgtccaggat
     4621 catggcgccc gagccggcgt gcctcggcag gccggtccgg ccgtagatgc ggcccttccg
     4681 ctccgggaag gccgcttcca tcgcctcgcg caatgtcgag gcggcgctgc cgacgaagcc
     4741 cgacagcgcg tcgttctcta tcctggcgcg catggccgac agcctcaggg agcggtagga
     4801 ctcgttcagc gtcgagcgga attcgagcgg cagcaggagc gaggccctga acacgctcga
     4861 gtcggccggc aggcccagtc cgaagacgag gctctcccag gccgcgacgc ggagggcttc
     4921 cgcggacgat acgcgaccgg ccgaggcgcc ggcccgttcg agggctctct cgagcgccgg
     4981 ccagtccctg gccctgaccg cgccggccac ggcccgctgg tcctcgacga acgcgtcgag
     5041 ggcctctatc tgctccccga ctctggctat gaatgtcatc aacgcttcga tgttcatatc
     5101 taccctcgct tcattatcgg tactggaaac gaggagttta cgccgatata gtaggtaggc
     5161 ccgcgtacgg gggcgaattt ctaatggggg acgggggcgc tagccgcttc cgggctcgag
     5221 ggatcgatgt tcaaagcgct gaccaacctg tattggctcg cccgcggcgt gcgcgtcttc
     5281 gcgctcgtcg gcggtagcgg caccggcaag agcttccgcg ccaagctcgt cgcccagaag
     5341 ttcggcatcg atatgatcat cgacgacgga ctgctgatca gcggcgacgc catcgtggcc
     5401 ggcaagtcgg ccaagcgcga gaaactgtac atggcggcgg tcaagaccgc cctgttccac
     5461 gagaaggccc atcgcgacga ggtggcccgc gtgctccaga agacgcgctg gaagaagatc
     5521 ctcatcatcg gcacgtcaga gaagatgacg atgaagatcg ccgagcgcct ccagataccg
     5581 tacccggcca agaccatcaa gatcgaggac atagccagcc gcgaggagat cgacaccgcg
     5641 atccgcgccc gcaaggtcga gggcaagcac gtcatccccg tgccggccat cgagatcaag
     5701 cgcagctacc cccgtatctt ctacgacagc atacgggtgt tcctgcagcg caacctgtcg
     5761 cccgggggcc tcacgacgcc gaagctgtac gagaagtcgg tggtgcgccc cgaattctcg
     5821 aagcgcggcc gcgtcgccat ttccgagtcg gcgctgtcgc agatggtcat ccactgcatc
     5881 gacgaattcg acgatgcgat ccgtatccag aagatcaccg tccggtcgga ctcgcaaggc
     5941 tacaggctga ccgtcgtgat agaggtaccg ttcggctctc agctgtccgg caagatccac
     6001 aatctgcagc agtacatcat cgacaacatc gagcgcttca ccggtatcct gatcgaggag
     6061 gtgaacctgg tcatcgaccg gttctccaag taggatgttc ggtcccgttt gacaacggac
     6121 tggacggggt ttaggataaa cgtaccatgg agggtaccat agtgaagaaa atactgagct
     6181 tcgcggtcat cgccctggcg ctggccgtgt gcgccggcgc gcaggatgcc gcgggcgtga
     6241 ccaatcccgc tccggcggcg gcgccggtag ccgctcccgc ggctaccgcc gaggtcgtgg
     6301 ccgttccggc cgcgcagccg accgctttca gcagcgccag ctccaagcgc tacgccgtct
     6361 ggtccgacgc cggcaaggat gacgccgtca ggctcgcgac gaccctcgac gggctcttcg
     6421 ccgtgtacaa cgagtacttc cgcttcgacg aatcggccct caaggccccg ttgacggtca
     6481 ggaagttcgc cgagaaggcc tcgttcgacg cctacctggc caaggtgata ggcgagacca
     6541 aggacgactt cgtctacctg cactacccga cgatcgagcg ctccgagctg gtcatcttcg
     6601 gcaaggccag cgccgacgac ttcgacgcct cgctcgccca ccaggccttc gtgcagttcc
     6661 tcaaggcctt cgtcccggag cctccgctct ggatccggga aggcttcgcc gtattcttcg
     6721 agcgcacccg ggccaatccg tccaccggcg tcgtagccta cgccgagaac cagtcctggc
     6781 tcgagaccat caagctgctc aagtcgcagg atcggctcta caccgtctcc gagctgatgc
     6841 tcctgtcctc cgacgaggcc aagagcgggc tcgacgtgtt ctacccgcag tcctgggccc
     6901 tcgtctcgtt cttcatcaac agccccgacc ggcgctataa ccgtttcgtc tgggacgcca
     6961 tcggcctcct ggcccgcgac gcgacgctcg agcagaacca gaacgccgtc cgggacttct
     7021 tcctgcgctg gcacgggcag gaagcggcgg agtcggactt cctgacctac ctggacggcc
     7081 agaagacctt cgccgagctg gtgacctccg gggttcgctc ctacggcgac aaggacctcg
     7141 agaccgccta caaggccttc tcccaagccc tgaccaagaa cgccgccagc tacatcccgc
     7201 actactacct gggcctgatc gcctacgccc gcggcgacta cgcgctggcg caggactact
     7261 atctcaaggc cctcgagctc ggtagcgacg cgcccatcac caactacgcg ctcggcatca
     7321 acgcctacgc ctccaggcgc ttcgacgagg cccgggccta cctgcagaag gcctcggacg
     7381 gcgatccgac caagtaccag gagaaggccg gcgagatcat cacccgcatc tccagcggca
     7441 tgtgatacga acgagccgag cgtatcgagc tacaaaagcc gccccgagag gggcggcttt
     7501 tttgtggcga acgcttgcat atggggcttt tccggccggc gcttagacct cgagctcgag
     7561 cccgtcgtag gccgggccgg ccgggaaggg caggccggcg tcgtcgcagt agctctggag
     7621 tcgctcatgg gacaggtcgt ggcagatatg ggtcaggagc agcctggccg gccgcagctc
     7681 gagggccagc gccagcgcct cgtccaccga caggtgcgtc gggtgcggcc tgagcctcag
     7741 cgcgccgagc acgagcaggt cgaggccgcg tagcatagcc ttcgacgagt ccgggacgga
     7801 cgagcagtcg gtcgcgtagg ccaggccgtc gagcctatag ccgaggacgc gccgcccgcc
     7861 gtgcgagagc ggaacggggc ggacgacgag cgagccgacg ctgacctgcc gcggcgcgga
     7921 gcccgttccg tccggcccgt cgaggtcgag gagctcgagc cgcggcttgc cgcctccggc
     7981 ctggccggcc gagaacacgt acgaaaagcg gtgccgcagc tcggacgcgt ccagtccgga
     8041 ggcgtagacc ggtagcggcc ggccccgcgt cagcgggcgc acgtcgtcta taccatgcac
     8101 atggtcggcg tgggcgtggg tgacgaggag cgcgtcgagg gcgtcgaggt gggcggcgag
     8161 cgcctgtaac cggaattcgg ggcccgcgtc gagcaggatg cgctcgccgc cgtcgccggt
     8221 caccagggcg gaggagcggt agcgcgtgtc gcgcgggtcg gtggacgagc atacggggca
     8281 ggagcagccg agcaccggca cgccgtggct cgtgccggtg ccgaggaagc gtatcctcac
     8341 ggtcggtcca gggcctgggg ttcgaagcgg ctagccgccg gcatgcgcac gagcttgacg
     8401 aactcgtagc cgcgccgcgt caccgcgtcg agggacgtct ccgccgcgct ctcgaagacc
     8461 gccaccctga aggcgccgtc ctcgtcgaag tacgggaaga gggcggcgat atggaaatag
     8521 cgccgcaggt tgggtggggc gctctcgcgg gcgttgaact gggcgaggta gaagcggccg
     8581 ggctcggagg atgccagcat gaacagcacg gccttgaggc cgcgcacgtc gaggccggcg
     8641 tcgtcgaagt tgtccgaata cggctcgtag gccgagccgc cgttgaccgg atcggcgtcg
     8701 gagacgcgca gcgcgaacgg cgcctcggcg acgtcgttgg cgagcggcga ctcgtcgcgc
     8761 agcgacgggt agaaggcgga ccaggccgcc cgggccaacg cgcgcgacca gtcgagcccg
     8821 aagaacgggt cgtatcgctc ctcgaagccg atggtgaacg aggagccgcg gcggtcgagc
     8881 atgcgttcct tgagcctgcc caccgccagg tagttccccg taataggatg caacatcccg
     8941 tcgaccagcc atttcacgaa gcccgagcag ttgaggccgg cgggtttcgc ctggtcggcg
     9001 agcgtcgaga tgaggacggc ccgcccgtcg gcgtctatcg ctccgtcgtc ggcgtagcgc
     9061 agcgacggca gcccggcgcg aaccgaggcc atgaggccgc gcaggtcccg atacagcgcc
     9121 gggtcgggcg agaatctgct ccagtcgatc acgtcggagg tcaggccgac gatccgcgag
     9181 aacggcgagc gcaacagctc ctcgaagggg agcggtatga tcgccttgtg gtagaggacg
     9241 ccgccatagg ccacgacgtc gatcctggag cgcgagccgt aggggtacag cctgacgaag
     9301 gtgccggggt cgctccggac gaagaccttg gcctgcagga ccgagccgtc ggcggcggat
     9361 cgcttgatga tccagctacc ctgggcgtag agcggcccgc cgcccggctt ggccccgccc
     9421 gagccgggcg cgaagatgac gtagaaggcg tcgccgccgt cgatcaccga gatcgaccag
     9481 gggccccagc cgttcctggc cgcggcgcca gggtaggcga gcgcctgggc cttggggcct
     9541 tcgatgagcc tggcccaagc gccgatccgt atgtcgtcgt tctcgggcat ggacgagatg
     9601 ggcctgtcgc cgccgagcga gccggccgcg ccatcggccc gggccggcgc gacggacgac
     9661 aaggccgcga gcatcgtgag cgccgctacg agggcgcggc agcaaggtcg actatcgtgc
     9721 ttcaatggtg ccatcgctca tagtatcggc ccgatcggcc gaataggcga gtgccgcctg
     9781 ggccgtagcg tcagcggttc aggttctacc gacccgagcc gatagtctaa gggaataacg
     9841 ccgatcaagg atggtccatg cccgcgtccc ccatgcatag gtcaacgctc gtatctatcg
     9901 cggtgttcgt atccgccgcc ctcgtcagcc tcggttcgtc gtctcagttc tccgagagca
     9961 tcggcgccgg ccccgaagcc gcttcggcct cgggacagga agcggcgcag gcggcggccg
    10021 gctctgcctc gacctcggcc tcgacctcgt cgacggcgat ggcgatggcg atggcgaccc
    10081 ggcccgctac ggcggacgcc tcgtcagcga ggacggcgga gccgtaccgg ctgttcccgg
    10141 ccctactcgg agccgtaccc gagccgagct cggagggccg cgtcgtctac ggtccgcgtc
    10201 cgcccatgag cgccgaggac gccgaggctc tcagacggtc caccgagctc gcggccgccg
    10261 aggcggccga gcgtacgccc gtgctcgccg gcaaccaggt gctcgccttc tacggcaagc
    10321 ccggagcccc ctcgatgggc atactcgggg agtataccaa ggaacagctg gcgccgctgc
    10381 tcgacggcta cgctaggctg tacgacggcg tcaacggcga tctcggggtg gtgccggcct
    10441 tctatatcat ctacggcacc tgctggccgg ccggggagat aggcatcctc aaggactcgg
    10501 tcgtccgctc gtatatcgag ttcgccgcgg agcgcggctg gctcgtcttc ctcgaccacc
    10561 agctcggtaa gtacggggtg gaggagtcgg tccgctcgat gctgccgtac ctgtcctacc
    10621 cgaacgtgca cctggcgatc gacccggaat ggcgaaccct caagccgatg gaggagatag
    10681 gctcgatcac cggcgctgag ctcaatgagg cccagcgcct catcgacgcc tacctgcgcg
    10741 accacgggct gccggggata cgcatgctcg tcgtccatca gttcaagccg acgatgatac
    10801 gcgactcggg gagcgtgaag gccggtttcg accgggtcgt gctcgtccat accgccgacg
    10861 gcttcggctc cccggccctg aagcgcctga cctacgcgag caacgcgcgc ctgtccaaca
    10921 tgccggtcaa gggcttcaag ctgttcttcg agtcgaaggt cgtgggcgcc ggctgggaca
    10981 agcccctgat gaagccggaa gaggtgctgg ccctcgaccc gatgcccctc gtcatcatgt
    11041 accagtgatg agtgtaaccg ttccgcgcgg cattgcatta tactgttaac cgtaccccgt
    11101 cttgagaagg agcgcccgta tgatcctccg agcaaagccc ctggccttga tcgccatagc
    11161 gtcgttggcc ctgtcgctgg cggcggccta cgacccgcct agcggcgccg agacggccgc
    11221 ctcgctcgcc tcacccgcgc tcctcgcagg aggctactcg gccgcctcga ccgaatcgcc
    11281 gatggcggat ctggccaatc cggccgcttc ggccgcgcaa cagcgcctga ccctcgatct
    11341 gagctacggc gccctcgtcg gcctggccga cgataccggt tggggcagcg ccctgaacct
    11401 cggcatagcc gtacctcggg cctacggcgt ctggaccggc tatctcggtc tcctgtcgtc
    11461 gcccttcggc tcgatgcccc tcggcaccgt ggtcacgggc cgcgtcgggc tggccaagga
    11521 tctgtacccg gacctgctgc tcggactggc cgtcgatacg cggctcggct cgaacggagg
    11581 tttcggctgg ggcctcgtcg ccgacctcgg agtgataggc ctggccggcg acgtcggcgc
    11641 gctcaagaac ctcgtctggg gcgcctcgct gcgcaacatg ggcaaggcgt tcgaggctcc
    11701 cggcgcggtg gggctgtcgg gcgggctcgc ctcggcctat gacgctccgt tcaccccgca
    11761 ggtcggcgcc tccgccgacc tcatacgggc cgacaaggcc ggcctcaagc tgtcggccaa
    11821 cgccgaccta tggttgccca ccttccagaa cgccgtcttc gcgacgggcc tgaggctcgg
    11881 ctggcgcgac cgcgccttcc tcaggctcgg ctgggacttc aacctgcgcg aggcgatcgc
    11941 cggcgtcgac cagggcctgc tacccagctt cggcctcggc gcgagcttcg ccatcgaccg
    12001 cgagagcgac gattcgctcc tctcgaaggc cggcctggac cgttcggaga tccgcccatc
    12061 cttctcggcc gccgagctgt acggcggcgt atgggccttc tcgggcggag cgaacgtgcc
    12121 ggtcggcgta ctcgatcggg tcccgcccag gatcaccgtg agctatcccg cggtcgagca
    12181 tgacgcctac tacatcagcc ccaacagcga cggcgctcgc gaccttctga cgctgccgct
    12241 ctcgatcacc gaccagcgct acgtccagag cttctcgctc aaggtactcg acgagtcggg
    12301 cgccgtggtc aagaccatgg ccaataagga gtccaggccc gagaaccagg acctgaaggg
    12361 cctcctggga cgcctgctct acgtgaagaa gggagtcgac gtcccggccg agctcgagtg
    12421 gaacggccta accgactccg gcgccgcggc cccggatggg cgctacttcg tcgtcatcga
    12481 ggcggtcgac gataacggca acgtggcctc caccgagcgc tggccggtcg tcatcgatac
    12541 tgtcccgccg agcgtcaagg cctcgggtcc cgccggggac gccttgattt tcagcccgga
    12601 cggcgatggc agcaaggacg agttcgccat agcgcaggaa gggtccgtcg aggactcctg
    12661 ggttgccgtc gtcaccgacg ccgccggagc gacggtacgc tcctggacga ccgcgtcggc
    12721 cgccccgtcg gccctgcgct gggacggcaa gaacgactcc ggcaaggtgg tgcctgatgg
    12781 cgtgtactcc taccgtgtcg ccgccgtcga ccgggccggt aaccagggct ccgccaggat
    12841 agacaacatc atcgtcaata cccagcagcc gccggtcggc gtgtccatcg acctggccgc
    12901 cttcagcccc gacggcgacg gcgtcaagga ctctctgacg ctgacgccga acgtgccggt
    12961 caaggccggc ctcgcgtcgt ggcgcctcgc cgtcctcgac gacaagggcg tcgagcgctg
    13021 ggccgcctct ggcgtcggcg ccgattcgat cgagccggcc taccgtttct cgggtaagga
    13081 cgcctccggg gcggccctgc ccgaggggac ctaccggacc cggttgtcgg tctcctacgt
    13141 gaacggccac ggccccgtgg cgc
//
LOCUS       DAOPHV010000006        12983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_5648_length_12983_cov_3.743038, whole genome shotgun sequence.
ACCESSION   DAOPHV010000006 DAOPHV010000000
VERSION     DAOPHV010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 12983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12983
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5648_length_12983_cov_3.743038"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..2183)
                     /locus_tag="PLQ29_00355"
     CDS             complement(114..2183)
                     /locus_tag="PLQ29_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HPG85123.1"
                     /translation="MRTARASDLSMVGRLSDFPLLDAVYVGTVRSPVSRGAIRSISVP
                     HLPRGYRSVTADDIPGSARIDSIGASVPVLASGRVAYRGEPVALIVGPDRLRVAEAVS
                     MTAVRVDEEPASFGYEACESRRLVAAYSTDSGSVDAALAKAARVVDGEYRMGPQDHYY
                     PEPQGAAAGFDYDKLIVFSSTQWPFQARDAVAQALGVRKDEVVVRTTSLGSHLDGKLW
                     YPSLLACHAAIAARLSGKNALLLLSRTDDFMYTTKRAPTLVSYRAGLDEAGKLVAMDA
                     SVMINVGAYAPFGAELVERAAKAVAGAYACPSLRVAARAVSTDLPPMGAFMGLGTGPV
                     AFAVERLAEDLADAAGMDPYEWRAMNVARRGDQTLGPPLRKGAPLDAVCDRLLAISDY
                     PRKRSSFELIRKRRPDPSSPPGYGIGLAFGPQRPATALSCRDAQAPSVEVTLSKDSTL
                     TIRSSVASGSGAVTRIWKRIAATSLGLSPEAVEVATPSTDEVPDSGPNVFSRRIGVVT
                     RLIGSACDGLASKRFREALPITVRKSQRARSAKAAPESPLDDASWGAAVVELELDPVD
                     AAPTVRGIWLVAKAGRILSREDAAAALERDAAVALGLCVAECLDLSAGPVDPGDLRRY
                     GLWRIKDAPPIQVEFVDDDAEPKGIGELAYSLVPAAFANALSQALDSPWNALPTCRRS
                     EGVDDDS"
     gene            complement(2180..3349)
                     /locus_tag="PLQ29_00360"
     CDS             complement(2180..3349)
                     /locus_tag="PLQ29_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HPG85124.1"
                     /translation="MNPNTDFIPFARPSIGPEEEEAVLRVMRSGWLTTGAEALAFEKE
                     FADFVGAPRALAVNSATSGLHLALEALGIGPGDVVVTSPYTFTSSAAVIRHLGAEVAF
                     CDVAPGSYNIDSDALGRLLATTKNCRAIVAVHVGGLPCDLRALRALADRYGCALVEDA
                     AHAFPSKTADGYAGAIGDVGVYSFYATKTITTGEGGMLVTRDGRLAARAEKMRMHGFD
                     RAAWDRYTAKKASWRYSVVEAGFKYNLPDLLAAIGRVQLTKAERFLEERRAIAALYDE
                     AFSGVRGLELPPRGDGHAWHLYSLRTLPERGGLARDEFIESLAERGVGTSVHFIPLHT
                     MPYYAGRYGLEPGSMPNAMAMFERTLSLPIWQGMGLDAARRVADAVLAVAEALHA"
     gene            complement(3333..4814)
                     /locus_tag="PLQ29_00365"
     CDS             complement(3333..4814)
                     /locus_tag="PLQ29_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HPG85125.1"
                     /translation="MNPDNPRIYIIGAGFAGRAIAREIRAKAVLGTIVAFLDDDEGKL
                     GRRVDGVPVLGPIRDVVRLLAREPADEAIIAMPSAPRELLRELYFTLKHAGFARIRIL
                     PDVAQIVEGDAHLVQAREIDPQDLLGRSPVSIGLKKSIEWLAGKRVIVTGAGGSIGSE
                     LARQLLSAGVERLYLFGHGENSIYQIDRELRGLQSGGVGVSTAIVPVIGELKDRDYMT
                     DVCRRLKADVVFHAAAYKHVPMMEANTVAAIENNAFGTRNLVSACLASGVKRFVLVST
                     DKAVEPLCVYGASKFLSERLVLDAQDRAGAGEHYMVVRFGNVLGSRGSILPLFKGQVE
                     RGGPVTVTDPAASRYFMTIPEACSLVLKTGGVGLGGESYLLDMGEPILIRELAEQVIR
                     FSGLRPYDDIAIEYIGLRPGERLEERLYSPDESLAATEFHKINRLERHGPRFDLEAAM
                     RALEPICFRSDDRPDAYRNRRRLRAALRSFIPTVEELPDEPEY"
     gene            complement(4795..5355)
                     /gene="loaP"
                     /locus_tag="PLQ29_00370"
     CDS             complement(4795..5355)
                     /gene="loaP"
                     /locus_tag="PLQ29_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator LoaP"
                     /protein_id="HPG85126.1"
                     /translation="MEYFAVQVWTGKEDEFADALSADPRVEAKAMVPKRALEIRRAGK
                     VRREERPLFPGYVFIATDADRLSDDQRWLLRGSRNFVRALPNNAEPRPVKEKDRRLLA
                     HFMSFGKVADISKVRFDADDRIVVVEGPLKGLEGLIVRVDARKRRAKIRLDMCENSFL
                     VDMGFEILDRAAKGTEGSDGDESRQP"
     gene            5510..6028
                     /locus_tag="PLQ29_00375"
     CDS             5510..6028
                     /locus_tag="PLQ29_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85127.1"
                     /translation="MRRLAIAAFFALAAAAPRLVAQTIEVSVMERGVEPDGPASLSSE
                     HVIAGMLDGLFESGFIATNARPAPGDDASFEAFSPGADAVEGFVDFVILVLAEYAPET
                     VVPACSYRILRVPDGAEVSRGKVPAATPVSKASIDIEKACMAVGSAISAACGGALRSL
                     SVSRGDDGYEEA"
     gene            6015..8411
                     /locus_tag="PLQ29_00380"
     CDS             6015..8411
                     /locus_tag="PLQ29_00380"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPG85128.1"
                     /translation="MKRHNIALTLGLAALVALASASSWEGSAMMGAYGDFPSSGYYAA
                     CNSFARNTSIDVVNLENGRSVTVIVTRGLESSGVFMMLSVEAAEALGLQRGGVTRVRA
                     SEPRSAVELAPSAGTSASMDPDINPRLLAAAELRRLGYELAPAAPSTSRQPAVSAVRV
                     QPETPVKAAPTVADPPAPAVSAPAIPVSPTAAAEARPQAAAPAVASAPPHVAAPSAIG
                     REAPAVAPASSRVAIAEPKPIGKETPESLASSKPRPVRTIVLPQLPEPEGPTTKAAVA
                     APVATPQSAPEAPAATAPEAVAMSLPVPDETPLRVAVGATPKRYESPAARPDVAVRGP
                     SAPALRVPAIALADPVTVSEARAAAFALAVPERYKPQASADLRDPMAPSSESASALAL
                     NSPSRASFDGSYAMYDPAVPDAERAEAFARAAPSLGQARLGLELAWPELEADEIPEVM
                     LAGLSAPVHDIPATSLAEGEILVPASVGPSALALETPEFGAAETYVAMEDAELKPEEK
                     PSVVMSGGLGPYGEEVAVELAEAEGKPEESPVVNVAAGLAPMGDEAYELAEAGRQPAV
                     DAAAGLEPYAEKADVALSEAEATATGGPEVEAMATGTPVDGAAGVELAEAGRQPAVDA
                     AAGLEPLAEKADVALADASEQKPETAPYVVEPTEEKTPAVALDSPKEYLVAIEPTGPK
                     PPVQTAPAVETKPAGTKVAPAAPAPAASAPAATIVSGALEKGRYYIQIGAYASEAAAK
                     DAASRLSVGYMVVVQQVTTGGKATWKVFVGPLSRDESGVALVRVRSMGYRDAFVKSGG
                     "
     gene            complement(8418..9605)
                     /locus_tag="PLQ29_00385"
     CDS             complement(8418..9605)
                     /locus_tag="PLQ29_00385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG85129.1"
                     /translation="MPRGAGFFSGKMAVILPSAFINSLAVGCMSLGMLFAVKDLFGAG
                     PGLVASLGALWSTAYLTGCIVLRVPARRLRPRTAMTVMLLGSAASVTAFLAYPGLWQA
                     FASYACFGFLTAFFWPPMMGWLSKGYEGAELNRATSMFSFSWSIGGIISSYMAGLLSE
                     RGKLLPIIVAAALLLVNAAFVTVSRLFVRLDEEPSAEAAEVEAVVDRSTPLRYPAWLG
                     VFLAYAVMGVVINVFPVFASDELGLSESTIGLALTIRAAATTVGFLALGRLTFWQFKR
                     AAIPALSIAAAATVALLAAARNAAGFALGFTVIGLLQAMVYNNSLFYGVSGASDRDKR
                     ASVHESVLTFGQITGSVSGGFVYQALSMPPVFIGLALLLGAGAAAQAVMVYAGHRHPK
                     ARV"
     gene            9809..11740
                     /gene="ligA"
                     /locus_tag="PLQ29_00390"
     CDS             9809..11740
                     /gene="ligA"
                     /locus_tag="PLQ29_00390"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPG85130.1"
                     /translation="MESDETRTARIGELERLVVHHQDLYYNGRPELSDAEFDALWDEL
                     STLAPDSPVLAKVGADAADGFPKARHVLPMGSQAKAADPEAFLAWCSKASLPEYVVEH
                     KLDGASLELQYESGRLVRAVTRGDGRVGDDVTPNARRMAGVPATLPGAWSGAVRGEVV
                     MSRATYRDHFPDKANCRNAANGIMKRKDGVGAERLEVVCYDAAPRGLYDGESGGLFAT
                     APEPPFDDELDKIAWLRAAGFDVAEPEVFATPYEVIDYRARVMDARESLRFDIDGLVV
                     KARKVDVDDMRRARPERQIAFKFPLEEATTILVDVEWSESGANYTPVGIIEPVRLAGT
                     TVRRASLANLSVLRGMGLRLGARVAVVKRGEIIPKIEGLVEDVQGSRDIPVPETCSCG
                     AALADDGTRLFCPNAACPKKRLHRLEKWLATLDVMEFGSTILRRLFDSGRLTTIADLY
                     GLEVGELAAYERMGEASATKIVQNLRARDAVTLAEFVAGFDIEGLGLLSMEKVQAAGY
                     DTLEKLRAATPEELSGVDGFGEVMAEALAAGLVELRDEMDAALATGRLAIKPPSTSSG
                     VLSGLSFCFTGELATMKRSRAQAIVKSLGGSVKGSVAKGLSYLVTNDPASGSEKSRKA
                     AALGVEVIDERAFLAMIGS"
     gene            11801..12961
                     /locus_tag="PLQ29_00395"
     CDS             11801..12961
                     /locus_tag="PLQ29_00395"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPG85131.1"
                     /translation="MSRLYQMRKFVVPETVFGDGAAYLAGRYAKNLGAARPMLVSDQG
                     LVSAGWTSAVVTACEAEGLEPVLFTEVSPNPRSTEVRLGVEAFEDGACDAIVAVGGGS
                     PMDAAKGIVASVANGVDVLAFEGVDKVRLPGPPLVCVASTAGTGSEVSRFAIVNDVDR
                     QVKIAIVSPKMVPDAALVDPRLLSTVPTALAAATGMDALTHAFEAYVSTASSALTDID
                     ARHAVRLIAANLAKLIADPLDDDARGAMALASMLAGRAFSNAGLGVVHAMAHALGGLL
                     DAPHGLCNALLLDAGIEVNFEVAGDRYADLALDLGASPGDIASGGVKARLLELVRGLR
                     ARVGIAAGLSGIGVSRADLSRLAGFASMDACLATNPVSLSADDIERIYERAL"
BASE COUNT         2076 a         4404 c         4483 g         2020 t
ORIGIN      
        1 cctcgcggca atcgctcata aggctcgtca ggtcgaagcg ctcgcgtagg agatcggata
       61 gccgctcgag ggggtcggcc ttcaggctga cgtcctctcc attcaggatg aattcaacta
      121 tcatcgtcga cgccctccga tcggcgacag gtcggcaacg cgttccacgg cgagtcgagc
      181 gcctggctca gcgcgttggc gaaggcggcg ggtacgagcg agtaggccag ctcgcctatg
      241 cccttgggct cggcgtcgtc gtcgacgaac tccacctgaa tgggaggtgc gtccttgatc
      301 cgccagaggc cgtatcgcct gaggtcgcca ggatcaaccg gcccggcgga caggtcgagg
      361 cactcggcga cgcaaagccc gagcgccacg gcggcgtcgc gctcgagcgc cgctgcggcg
      421 tcctcgcgcg acaggatgcg gcccgccttc gccacgagcc agatgccgcg taccgtcggg
      481 gcggcgtcca ccgggtcgag ctcaagctct acgacggcgg cgccccatga cgcgtcgtcg
      541 aggggcgact cgggcgcggc cttggcggac ctagcccgct gcgacttcct gacggtgatc
      601 ggcagcgctt ccctgaagcg cttggaggcc agaccgtcgc aggcggagcc tatcagcctc
      661 gtcacgacgc cgatccgccg cgagaacacg ttcggccccg agtcgggtac ctcgtcggtg
      721 ctgggcgtcg cgacctccac cgcctcgggc gagagtccga gggacgtcgc ggctatccgc
      781 ttccagatcc tcgtcacggc gcccgagccc gacgccacgc tcgagcggat ggtcagcgtc
      841 gagtccttcg acagggtcac ttcgaccgag ggagcctggg cgtcgcggca ggaaagcgcc
      901 gtggccggcc gctgagggcc gaaggccagc ccgatgccgt agccgggcgg ggacgacggg
      961 tcggggcgtc gtttgcgtat cagctcgaac gacgatcgct tgcgcgggta gtcgctgatg
     1021 gcgagcagcc tgtcgcagac cgcatcgagc ggcgcgccct tcctgagcgg tgggccgagc
     1081 gtctggtcgc cgcgcctggc cacgttcatg gcgcgccatt cgtatggatc cataccggcg
     1141 gcgtcggcca ggtcctcggc caggcgctcg acggcgaagg ctaccggccc ggtgccgagc
     1201 cccatgaagg cgcccatcgg cggcaggtcc gtcgagaccg cccgggccgc gacgcgcagg
     1261 gatgggcagg cgtaggcgcc ggcgacggcc ttggcggccc gctcgaccag ctccgcgccg
     1321 aacggcgcgt aggcgccgac gttgatcatc acgctcgcgt ccatcgctac gagtttgcca
     1381 gcctcgtcga ggccggcccg gtacgagacg agggtcggcg cccgtttcgt cgtgtacatg
     1441 aagtcgtcgg tcctcgatag gagcaggagc gcgttcttgc cgctcagcct ggcagcgatc
     1501 gccgcgtggc aggccaggag cgacgggtac catagcttgc cgtccaggtg ggagccgagc
     1561 gacgtggtcc ggaccaccac ctcatccttc ctgactccga gcgcctgggc gacggcgtcc
     1621 ctggcctgga agggccattg agtcgacgag aaaacgatca gcttgtcgta gtcaaagccg
     1681 gccgcggcgc cctggggctc cgggtagtaa tggtcctgcg gacccatcct gtattctccg
     1741 tcgaccaccc gggcggcctt ggccagcgcc gcgtcgacgg agccgctatc ggtcgagtag
     1801 gcggcgacga gtctcctcga ctcgcaggcc tcgtatccga acgacgccgg ctcctcgtcg
     1861 acgcgtaccg ccgtcatcga tacggcctcg gctaccctga gccggtccgg cccgacgatc
     1921 agcgccaccg gctcgccgcg gtacgcgacg cgccccgagg ccaggacggg caccgaggcg
     1981 cctatactgt cgatacgggc cgagcctggg atatcgtccg ccgtgacgct ccggtagccg
     2041 cgtggcaggt gcggcacgct gatggatcgt atggctcccc tcgatacggg cgagcgcacc
     2101 gtcccgacgt agaccgcgtc gaggagaggg aaatccgaca ggcgccctac catcgagagg
     2161 tcgctcgccc gggccgtcct catgcgtgca gcgcctccgc gacggctagg acggcgtcgg
     2221 cgacgcgccg cgccgcgtcg agtcccatgc cctgccagat cggcaggctg agggtgcgct
     2281 cgaacatggc catggcgttg ggcatggatc ccggctcgag gccgtagcgc cccgcgtagt
     2341 agggcatggt gtggagcggg atgaaatgga ccgacgtgcc gacgccccgc tcggcgagcg
     2401 actcgatgaa ctcgtccctg gctaggcctc cgcgctccgg cagggtacgc agcgagtata
     2461 ggtgccaggc gtgcccatcg cccctcggcg gaagttcgag ccctcgaaca cccgagaagg
     2521 cctcgtcgta cagggccgcg atcgcccgtc gctcctcgag gaagcgctcg gccttggtca
     2581 gctgaacccg tccgatcgcg gcgagcaggt cgggtaggtt gtacttgaag ccggcctcga
     2641 cgaccgagta gcgccaggac gccttcttgg cggtataacg gtcccaggcc gcccggtcga
     2701 agccgtgcat acgcatcttc tcggcgcggg ccgccaggcg cccgtcccgc gtgacgagca
     2761 tgccgccctc gccggtcgtg atggtcttgg tggcgtagaa cgagtagacg cccacgtcgc
     2821 ctatcgcgcc ggcgtagcca tcggcggtct tcgacgggaa ggcgtgggcc gcgtcctcga
     2881 cgagagcgca gccgtagcga tcggctaagg cgcgcagggc gcgaaggtcg cagggcagcc
     2941 cgccgacgtg gacggcgacg atcgcccggc agttcttggt cgtcgccagg agcctgccga
     3001 gcgcgtcgga gtcgatgttg tacgatccgg gcgccacgtc gcagaaggcc acctccgcgc
     3061 ccaggtggcg tatcacggcg gcgctcgagg tgaaggtgta ggggctggtg acgacgacgt
     3121 cgcccgggcc gatgccgagc gcctcgagcg ccaggtgcag gccgctcgtg gccgagttga
     3181 cggccagggc gcggggcgcg ccgacgaagt ccgcgaattc cttctcgaag gccagcgcct
     3241 cggccccggt ggtaagccag cccgagcgca tcacgcggag gacggcttcc tcctcctcgg
     3301 gaccgatcga cggccgcgcg aacgggatga agtcagtatt cgggttcatc gggaagctcc
     3361 tctaccgtgg gtatgaagga cctgagcgcg gcccgaagac gcctgcggtt acggtacgcg
     3421 tccggtctat cgtcgctcct gaagcatatc ggctcgagcg cgcgcatggc agcctcgagg
     3481 tcgaagcggg gcccatgccg ctccaggcgg ttgatcttgt ggaattccgt agcggcgagg
     3541 ctctcgtcgg gcgagtacag ccgctcctcg aggcgctcgc cgggcctgag cccgatgtac
     3601 tcgatggcga tgtcatcgta gggtctgagg ccggagaagc gtatgacctg ctcggctagc
     3661 tcgcgtatca gtatgggctc gcccatgtcc aggaggtacg attcgccgcc caagccgacg
     3721 ccgccggtct tgagcacgag ggagcaggcc tcggggatgg tcatgaagta tcggctggcg
     3781 gcggggtcgg tgacggttac cgggccgccg cgctcgacct ggcccttgaa caggggcagg
     3841 atggagccgc gcgaaccgag cacgttgccg aagcgtacga ccatgtagtg ctccccggcc
     3901 ccggcccgat cctgcgcgtc gagcacgagg cgctccgaca ggaacttcga agcgccgtag
     3961 acgcacagcg gctcgaccgc cttatcggta ctgacgagca cgaaacgctt gacgccggac
     4021 gcgaggcagg cgcttacgag gttccgggtg ccgaacgcat tgttctcgat ggcggcgacc
     4081 gtgttggcct ccatcatcgg gacgtgcttg tacgcggcgg cgtgaaacac gacgtcggcc
     4141 ttgagcctgc ggcaaacgtc ggtcatgtag tcgcggtcct tgagctctcc tatcacgggg
     4201 acaatggccg tcgacacgcc gacgcctccc gactggaggc ccctgagctc gcggtctatc
     4261 tggtagatcg agttctcgcc gtggccgaac aggtacaggc gctcgactcc ggccgacagg
     4321 agctgcctcg cgagctcgga gcctatcgaa ccgccggccc cggtgacgat cacgcgcttt
     4381 ccggccaacc actcgatgct cttcttgagt cctatcgaga cggggctgcg cccgagcagg
     4441 tcctgtgggt ctatctcgcg ggcctggacg aggtgggcat cgccctcgac tatctgcgct
     4501 acatccggga gtatgcgtat ccgcgcgaag cccgcgtgct tgagcgtgaa gtacagctcg
     4561 cgcaacagtt ccctcggtgc gcttggcatg gcgatgatgg cttcgtcggc cggctccctc
     4621 gcaagcagcc tgaccacgtc gcggatgggg ccgagtactg gcacgccgtc gacgcgcctg
     4681 cccagcttgc cctcgtcgtc gtcgaggaag gcgacgatgg tgccgaggac cgccttggcc
     4741 cggatctccc gcgcgatcgc gcgtccggcg aagccggcgc cgatgatgta tatcctaggg
     4801 ttgtcgggat tcatctccat cgctgccctc ggttcctttc gccgcccggt cgagtatctc
     4861 gaaacccatg tcgacgagga acgagttctc gcacatgtcc aggcgtatct tggcccggcg
     4921 cttgcgcgcg tcgaccctga cgatcaggcc ctcgagcccc ttgagcgggc cttcgacgac
     4981 gacgatgcga tcgtccgcgt cgaaacgtac cttcgatata tcggcaactt tcccgaacga
     5041 catgaagtgg gcgagtagcc tccggtcctt ctccttgacc ggcctcggct cggcgttgtt
     5101 cgggagcgcc cgtacgaagt tcctcgagcc acgcaataac cagcgctggt cgtcgctcag
     5161 gcgatcggcg tcggtcgcga tgaagacgta gcccgggaag agggggcgct cctcgcgccg
     5221 taccttgccc gcccgccgta tctcgagggc ccgcttgggc accatagcct tggcctcgac
     5281 gcgcggatcg gccgatagcg cgtcggcgaa ctcatcctct ttgccggtcc atacctgtac
     5341 ggcgaaatac tccatcgctc ctccgtgatc gccgttccgc ccgatgaacg aaccctcccg
     5401 aggctagcac gaaggggaga gggccgacaa ggctgagccc cgtatcgacg atacgcctaa
     5461 atcatgtcgt ccgtctggcc gatattccaa gaagaaaagg atgcgaacga tgagaagact
     5521 cgcgattgcg gcttttttcg ccctggcggc ggccgcccct cgcctggtcg cgcagaccat
     5581 cgaggtctcc gttatggagc ggggcgtcga acccgacgga ccggcgtcgc tatcctccga
     5641 gcacgtcatc gccggtatgc tcgacggcct attcgagtcc ggttttatcg ctacgaacgc
     5701 caggccggcc ccgggcgacg acgcgtcgtt cgaggcgttc tctccgggag ccgacgctgt
     5761 cgaggggttc gtcgacttcg tcatcctggt actggccgag tacgcgcctg aaacggtcgt
     5821 gccggcctgc agctacagga tactcagggt acccgacggc gccgaggtat cgcgagggaa
     5881 ggttccggcg gctacgcccg tctccaaggc gagcatcgat atagagaaag cgtgcatggc
     5941 cgtcggatcg gccatttcgg ccgcctgcgg cggcgcgttg cggagcctta gcgtttcgcg
     6001 gggggatgac ggatatgaag aggcataata tcgcgttgac gctcggcctg gcggcgctcg
     6061 tcgccctcgc gtcggcttcg tcatgggaag gcagcgcgat gatgggagcc tacggggatt
     6121 tcccttcgtc gggctactac gccgcgtgca actccttcgc gcgtaatacc tccatcgatg
     6181 tggttaatct cgagaacgga cgctcggtca cggtgatcgt gacgcgcggc ctcgagagct
     6241 ccggcgtctt catgatgctg tccgtcgagg ccgcggaggc gctcggcctg cagcgcggcg
     6301 gcgtgacgag ggtcagggcc tcggagccgc ggagcgccgt cgagctcgcc ccctccgccg
     6361 ggacgagcgc ttcgatggat ccggacatca atccgcgtct cctggcggcc gccgagctca
     6421 ggcgcctcgg ctacgagctg gctcccgcgg ccccgtccac ctccaggcag ccggcggtat
     6481 ccgccgtccg cgtccagccc gagactccgg tcaaggccgc cccgaccgtc gccgaccccc
     6541 ccgccccggc agtctctgct ccggcgatcc cggtttcgcc taccgccgca gcagaggctc
     6601 ggcctcaagc cgcggccccg gccgtcgcct cggctccgcc tcacgtagcc gcgccttcgg
     6661 ccattggccg cgaggctccc gccgtggctc ccgcctccag ccgtgtggcc atagccgaac
     6721 ctaagccgat cggcaaggaa acgcccgaat cgctcgcctc gtcgaagccg aggccggtcc
     6781 gtacgatcgt gttgcctcag ctgcccgagc cagaagggcc gacaaccaag gcagccgtcg
     6841 cggcgcccgt ggctacgcca cagtcggctc ccgaggctcc tgccgcgacc gctccggagg
     6901 cggtggcgat gtcgctgccc gtgccggacg agacgccgct acgcgtcgcg gtcggcgcta
     6961 ccccgaagcg ctacgaatcg ccggccgcca ggccggacgt ggccgtgcgc ggcccatccg
     7021 cccctgcgct acgcgttccg gccatagcct tggccgaccc ggtgaccgtc tccgaggcca
     7081 gggcggcggc cttcgcgctg gccgtacccg agcgatacaa gccgcaggct agcgccgacc
     7141 tgcgcgaccc tatggccccc tcgtccgaga gtgccagcgc cctggcgctg aactcgccgt
     7201 cccgagcgtc gttcgacggg tcgtacgcca tgtacgaccc ggccgtcccg gacgccgaga
     7261 gggccgaggc cttcgcccga gccgcgccgt cgctcggtca ggctaggctc ggtctcgagc
     7321 tggcctggcc cgagctcgag gccgacgaga tacctgaagt catgctggcc ggcctgagcg
     7381 ccccggtcca cgatatcccc gcgaccagcc tggccgaggg cgagatcctg gtacccgcgt
     7441 cggtcgggcc gtccgccctg gcgctcgaga cgccggagtt cggcgccgcc gagacctacg
     7501 tggccatgga ggatgccgag ctaaagcccg aggagaagcc gagcgtggta atgtccggcg
     7561 gcctgggccc ctacggcgag gaggtcgcgg tcgagctggc cgaggccgag ggaaagcctg
     7621 aggagagccc ggtcgtcaac gtggccgccg gactggcacc gatgggcgac gaggcctacg
     7681 aactggccga agccggaagg cagccggccg tcgatgcggc cgccggactt gagccctacg
     7741 ccgagaaggc cgacgtggcg ctcagcgagg ccgaggcgac ggcgacaggt ggtcccgaag
     7801 tggaggcgat ggccaccggt accccggtcg acggcgccgc cggcgtcgag ctggccgagg
     7861 ccggaaggca gccagccgtc gacgcggctg ccggacttga gccccttgcc gagaaggccg
     7921 acgtggcgct ggcagacgcc tcggagcaga agcccgagac cgctccctac gtggtcgagc
     7981 cgaccgagga gaagactccg gccgtagcgc tcgactcgcc caaggagtac ctcgtcgcca
     8041 tcgagccgac aggcccgaag ccgcccgttc agacggcgcc ggccgtggag acgaagccgg
     8101 cgggtaccaa ggtcgctcca gcggctcccg ctccagcggc ttccgctcca gcggcgacga
     8161 tcgtctcggg cgcgctcgag aagggccgct actacataca gataggcgcc tacgccagcg
     8221 aggcggccgc taaggacgcg gcctcgcgtc tgagcgtcgg ctacatggtg gtcgtccagc
     8281 aggtgacgac cggaggcaag gccacttgga aggtcttcgt aggcccccta tcccgagacg
     8341 agagcggcgt ggccctcgtg cgggtcaggt cgatgggcta ccgcgacgcc ttcgtgaaga
     8401 gcggcggctg attgcggtca gacgcgggcc ttcggatgac gatggcccgc gtagaccatg
     8461 acggcctgcg cggccgctcc ggcgcccagt aagagggcca agcctatgaa cactggtggc
     8521 atcgataggg cctggtagac gaagccgccg ctgaccgatc cggttatctg gccgaaggtc
     8581 aggaccgatt cgtgcacgct cgcccgcttg tcgcggtcgg acgccccgct caccccgtag
     8641 aacagcgagt tattgtacac catggcctgg agtagcccga tgaccgtgaa acctaaggcg
     8701 aagcccgcag cgttccgggc cgccgccagg agggcgaccg tggcggcggc ggcgatggac
     8761 agagccggga tggcggctcg cttgaactgc cagaacgtca gcctgccgag cgccaggaac
     8821 ccgaccgtcg tggccgcggc tcgtatcgtc aacgctaagc ctatcgtcga ctcgcttagg
     8881 ccgagctcgt cgctcgcgaa taccgggaat acgttgatca cgacgcccat caccgcgtag
     8941 gcgaggaata cgccgagcca agccggatag cggagggggg tcgagcggtc gacgacggct
     9001 tcaacctcgg cggcctcggc cgatggctcc tcgtccaaac ggacgaataa cctcgagacg
     9061 gtgacgaagg ccgcgttgac gagcaggagg gccgccgcga cgatgatcgg caggagtttg
     9121 ccgcgctcgg acaggaggcc ggccatatac gagctgatga tgccgcctat gctccatgaa
     9181 aagctgaaca tgctggtggc gcggttgagt tcggcgccct cgtagccctt ggacagccag
     9241 cccatcatag gcggccagaa gaaggccgtc aggaaaccga agcacgcgta cgaggcgaag
     9301 gcttgccaca ggcccgggta agccaggaac gccgtcaccg aagcggcgct accgagaagc
     9361 atgacggtca tcgccgtcct cggcctcagg cgcctggccg ggacgcgcag gacgatacag
     9421 cctgtcaggt aggccgtgga ccacagcgca ccgagcgacg ccacgaggcc aggcccggcg
     9481 ccgaacaggt ccttgacggc gaacagcatg ccgagcgaca tgcagccgac ggccaggcta
     9541 ttgatgaacg ccgacggaag aataacggcc atcttaccgg aaaagaaacc agcgcctctc
     9601 ggcatacgtc ccgccttcgg gtcctcgaac gagcccaagc gaacggtagt tcggccgggc
     9661 ccctcgggtc aagcggggat gccttcggcg tagtgccttc ggcgtagtgc cttcggcgta
     9721 gtgccttcgg cgttgaccgg gggcggcggc gcgtctacaa tgccgtttgc gccgagggcg
     9781 cggcagacga cacgcgaggc ggggagcgat ggaatccgat gaaacacgaa cggcgaggat
     9841 aggggagctc gagcggctcg tcgtccatca ccaggacctc tattataacg gtagacccga
     9901 gctatcggac gccgagttcg acgcgctttg ggacgaactg tcgaccctgg cgcctgacag
     9961 cccggtgctg gccaaggtag gcgccgacgc ggccgacggt tttcccaagg ccaggcacgt
    10021 gttgcccatg ggtagccagg ccaaggctgc cgaccccgag gcgttcctgg cctggtgctc
    10081 taaggcctcc ctgccggagt acgtcgtcga gcacaagctc gacggcgcca gcctcgagct
    10141 acagtacgag tccggccgcc tggtgcgcgc ggtcacacgc ggcgacggcc gtgtcggtga
    10201 cgacgttacc ccgaacgccc gacgcatggc cggcgtaccc gcgaccctgc ctggcgcctg
    10261 gagcggcgcg gtacgcggcg aggtcgtcat gtccagggct acttaccgcg accacttccc
    10321 ggacaaggcg aactgccgga acgccgcgaa cggtatcatg aagcgcaagg atggtgtcgg
    10381 cgctgaacgg ctcgaggtgg tctgctacga cgcggcgccg aggggcctct atgacggcga
    10441 gtccggcgga ctattcgcca ctgctcccga gccgcccttc gacgacgagc tcgacaagat
    10501 cgcctggctc cgggccgccg gcttcgacgt ggccgagccc gaggtcttcg cgaccccgta
    10561 cgaggtgatc gactatcgcg cccgcgtgat ggacgcccgc gagtccctgc ggttcgatat
    10621 cgacggcctc gtcgtcaagg ccaggaaggt cgacgtcgac gacatgaggc gcgcccggcc
    10681 cgagcggcag atcgccttca agttcccgct cgaggaagcg acgacgatcc tcgtcgacgt
    10741 cgaatggagc gagtccggcg ccaattatac gcccgtcggc atcatcgagc cggtccggct
    10801 ggccggaacg accgtcaggc gcgccagcct ggccaacctg agtgtgctcc gcggcatggg
    10861 cctcaggctc ggcgccaggg tggcggtcgt caagcgcggt gagatcattc ccaagatcga
    10921 gggcctcgtc gaggacgtgc agggcagccg cgacataccc gtacccgaga cctgttcctg
    10981 cggcgcggct ctcgccgacg acggcacgag gctgttctgc ccgaacgccg cctgtcccaa
    11041 gaagcggctg caccggctcg agaaatggtt ggccacgctc gacgtgatgg agttcggctc
    11101 gacgatcctg cgcaggctgt tcgactccgg caggttgacg acgatcgccg acctctacgg
    11161 gctcgaggtc ggggagctgg cggcgtacga gcgcatgggc gaggcgagcg ccaccaagat
    11221 cgtccagaac ctgcgcgccc gcgacgcggt gacgctcgcg gaattcgtgg ccggcttcga
    11281 catcgagggc ctgggactcc tttccatgga gaaggtccag gcggccggtt acgacactct
    11341 cgagaagctt cgggccgcca cgcccgagga actgtcgggc gttgacggct tcggcgaggt
    11401 tatggccgag gcgctggcgg ccgggctcgt cgagctgcgc gacgagatgg acgccgccct
    11461 ggcgaccggt aggctcgcca tcaagccgcc atcgacctcg agcggcgttc tatccgggct
    11521 gtcgttctgc tttaccggcg agctggcgac catgaagcgt tcgagggctc aggccatcgt
    11581 caagtcgctc ggtggatcgg tcaagggctc ggtggccaag ggcctgtcat acctggtgac
    11641 caacgacccg gcgtccggct cggagaagag ccgcaaggcc gcggccctcg gcgtcgaggt
    11701 gatagacgag cgggctttcc tggccatgat cggttcgtga ccctgcgact ataggacctt
    11761 cgtcgttgcg ctccgctcga agcgcgtcta tagtaatgac atgagccgct tgtaccagat
    11821 gaggaaattc gtggttcccg agaccgtatt cggcgacggc gcggcctatc tggccggccg
    11881 ctacgccaag aacctcggcg ccgcgcgtcc tatgctcgtc agcgaccaag gcctcgtgag
    11941 cgcgggctgg acctcggctg tcgtcacggc ctgcgaggcc gagggactcg aaccggtcct
    12001 gttcaccgag gtgagcccga acccgcgctc gaccgaggta cgcctgggcg tcgaggcctt
    12061 cgaggacggc gcttgcgacg cgatagtcgc cgtgggaggc ggtagcccga tggacgccgc
    12121 gaaaggtatc gtagcctcgg tcgccaacgg cgtcgacgtg ctggccttcg agggcgtcga
    12181 caaggtcagg ctgccagggc cgcctctcgt ctgcgtcgcg tcgacggcgg gtaccggctc
    12241 ggaggtatcg cgcttcgcga tagtcaacga cgtcgaccgt caggtcaaga tagccatcgt
    12301 cagcccaaag atggtcccgg acgcggccct cgtcgatccg aggctcctat cgaccgtgcc
    12361 gaccgcgctg gcggcggcca ccggcatgga cgcccttacc cacgccttcg aggcctacgt
    12421 ttcgaccgcg tcgtcggccc ttaccgatat cgacgcgcga cacgccgtgc ggctgatagc
    12481 cgccaacctg gctaagctca tagccgatcc cctcgacgac gacgcccgtg gggccatggc
    12541 gctggccagc atgctggcgg ggcgcgcgtt ctcgaacgcg ggcctcgggg tcgtgcacgc
    12601 gatggcccac gcgctcggcg gcctcctgga tgcgccccac ggcctctgca acgcgctgct
    12661 cctcgacgcg ggcatcgagg tcaacttcga ggtggcgggc gatcgctacg ccgacctggc
    12721 gctcgacctg ggcgcgtccc ccggcgatat agcgtccgga ggcgtcaagg cccgtctact
    12781 cgagctggtt cgcgggctta gggcccgggt tggcatagcc gccggcctct cgggcatcgg
    12841 agtgtcgcgg gccgacctgt cccggctggc cggtttcgcc tccatggacg cctgtctggc
    12901 caccaacccc gtgtcgttat ccgccgacga tatcgaaagg atctatgagc gggcgctctg
    12961 acctgcccgg cgacttcggg gac
//
LOCUS       DAOPHV010000007        12488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_5990_length_12488_cov_4.112684, whole genome shotgun sequence.
ACCESSION   DAOPHV010000007 DAOPHV010000000
VERSION     DAOPHV010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 12488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12488
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5990_length_12488_cov_4.112684"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLQ29_00400"
     CDS             <1..211
                     /locus_tag="PLQ29_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HPG85132.1"
                     /translation="ATITELANMITAQAVTKLHELGFRFDLTPPAIFTGDNMEVSNQD
                     VEALIVPMEAPQGKVEINVAIRERA"
     gene            222..659
                     /locus_tag="PLQ29_00405"
     CDS             222..659
                     /locus_tag="PLQ29_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPG85133.1"
                     /translation="MKTKEDFPSINERVPEGQRPDGTPFRVLVVDDSMFVAKQITQIL
                     TSEGYDVVGQAADGLQGLERYKELFPNVDLVTMDITMPNMDGITALQKIIEFDKNARV
                     IMISALGKQDLVKQALLTGAKNYIVKPLDRKKVLERIQHVLSK"
     gene            751..824
                     /locus_tag="PLQ29_00410"
     tRNA            751..824
                     /locus_tag="PLQ29_00410"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:785..787,aa:Val,seq:gac)
     gene            complement(899..2893)
                     /locus_tag="PLQ29_00415"
     CDS             complement(899..2893)
                     /locus_tag="PLQ29_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPG85134.1"
                     /translation="MRAICIQPPLVQLNAPYPAVWYLDAWLRSRGVDSRAEDHSIAMA
                     RRVFSRAGLETVFAAARDALRGRQHPDDETARRIGAYFDDEAAYLACVEPMTAFLSGD
                     DPAYAYRIAVGSGLPAGMRAEAVFGDGIGPDDARAFATATLNDLSDFIAYALDPSFGT
                     VRYAERLAASAPDFGPIRRAAHEGWVLREFYRPIARASMAKFKEGLRADDDALVLVTV
                     PFPGCLAGAVVAAEEAKAAFGDRATIVAGGGYVSTELRFLTDPAPFDAFDYLAYDAGY
                     GALASILDVVDGAPRSSLYRVRYREEEGRVVAAGFPADEPRSGATSSGAAGTAPAPAG
                     TASAPADSSKASGYERLEREAIAGSHPDYAGLDVSGYLRVVDSPNPMHRLWNDTPWLK
                     YRLAYGCYWRRCAFCDTQLDYIKRYLASDLDALEEAAGRAAGRTGLYGIHFVDEAMPV
                     NLVRAFAERNARRSRPFTFWGNARFDRAWTDESCAFAAERGLVAVSGGIEIATKKGLE
                     IVDKGFSMVDLVRTLASFKRCGVLVHAYLIYGFPGQSDQDIADSAEMARVLLAEGLVD
                     SAFWHRFVLTRHSAIYAAWERGERPGLEPLAPPSSFALNDLRFAGEERSERWTAPLDA
                     SLAAWIDDGETDRPLAAFLPRGLPRPRLDALALLREAGLA"
     gene            2979..3051
                     /locus_tag="PLQ29_00420"
     tRNA            2979..3051
                     /locus_tag="PLQ29_00420"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3012..3014,aa:Phe,seq:gaa)
     gene            3197..4162
                     /gene="galE"
                     /locus_tag="PLQ29_00425"
     CDS             3197..4162
                     /gene="galE"
                     /locus_tag="PLQ29_00425"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPG85135.1"
                     /translation="MKVLVIGGAGYIGSHVCLEMMEAGHEVTVYDNLSSGLRENLFEG
                     ERFIQGDVLDAEGLARAMSEGFDGVVHLAAFKAAGESMVAPEKYARNNINGTINILNA
                     MCGAGIGRFVFSSSAAVYGEPTYLPVDEKHPTVPENFYGFTKLEIERILGWYDRLKGL
                     RYSALRYFNAAGYDVRGRARGLERNPANLLPVIMEVAAGMRPELRVFGRDYDTKDGTG
                     VRDYVHVNDLAVAHVAALERMERTNESLVVNLGSEEGLSVLEILEAARRITGKPVPAT
                     IEGRRPGDPAKLVASSALAAEKLGWKARYSDVDTLVETSWKAYER"
     gene            4199..5431
                     /locus_tag="PLQ29_00430"
     CDS             4199..5431
                     /locus_tag="PLQ29_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HPG85136.1"
                     /translation="MDYIKQAIDYLDRAEAGVIELEKLLTAIPALAPESGGDGEWKKA
                     EALEAWLRARGIVDIERHDAPDARVSAGKRPNLVATIKGRKPGKRLWVMAHTDVVPEG
                     DRSLWDTEPFEAVVKDGKIYGRGVEDNQQGLVSSVFAALALVENGIAPERDVKLLFVA
                     DEEYGSVYGIQWLLENRELFSPDDLIVVPDSGSADGSEIEIAEKNVCWLKLATKGKQC
                     HASRPDDGANAFLANCELALSLNAMERESFPATDPIFVPDRTTITPTKKDANVPNINT
                     VPADDAFYVDMRILPRYSIDEVLAETAKRAAAVERKYGVKVGVEVVQRNDSPPTPADA
                     PVVTLLKRAIEETYGVAGRAVGVGGGTVGAYLRSAGFPCVVWSRQDETMHAPNEYTRI
                     SNLIGDAKIFARLMLAER"
     gene            5780..6619
                     /locus_tag="PLQ29_00435"
     CDS             5780..6619
                     /locus_tag="PLQ29_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPG85137.1"
                     /translation="MKSAMLFGAKELRRLTGISAETLRHYVDRDVLRPLRVDGNNYRK
                     FSSQNMIDVLHARIYRGLGLPIQSITRKSGSGPEGQDDVLARHQAILEREALELQMKL
                     ARMRQELEFLATARRRIGCVRVRTADEAPSVYRLTLLDAADGMISDAEVGRRVAEWMR
                     YAPHLHVALRVPEAAVLDPSVEELPVSLGLSVRSETASSLGMNAGPPAEYCPNKAHVG
                     TLLALADPLSLRRRDILPLLDTVRERGLRLEGGLIGRLCYIVDAEGGRLYYFSVNLCV
                     VSA"
     gene            6689..8935
                     /locus_tag="PLQ29_00440"
     CDS             6689..8935
                     /locus_tag="PLQ29_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG85138.1"
                     /translation="MMKMRRTYRTLVAAIAILVSVLAGCNSVGRGGSVYAPGTYSVST
                     KGFGGDVTVALTVDARSIVGVEIVGEDETPEIGGMAMERMARDIPAVGEAVEGVSGAT
                     YTSDAVKRALSEALARARGESVSVKLDVAPGSYGASAPGFDLSVPVPVTVGVVDGRIA
                     SIEIGACKETEGKLTAVRDRLIPRIIERQSLAVDAVTGATATSNAVIGAVRASLVAAG
                     ASEAALYAPVPRSSAKEAYTADVVVVGFGASGMTASLAAAEKGAKVIALEKSGRIGGT
                     AVVTSGPMAVNAPSMVGNRIVDWDDPLLKKKRVKEAGEPLVDAGALLRDWLQYTTVDG
                     VQGAKEGIIRRVVDRSGQTLDWLTGFGFEFSDARGFLGNQWALFSPFKGNKALTESFF
                     DKAVARFEKEFGGRCLVETEATSLIIEGGRIAGVRARKSDGTEVTVKAKAVILASGGF
                     GGSDEMMEQYLGESWKMYGMEQNEGDGIRMALSAGAATYNIGMPPMSHFSAPSVILRS
                     FDSDFDNDIPFGMVATSETLAVNKSGVRFINEANIGLDAYLGGSRFYTIYSKEQIDVL
                     RARGFGFAATGRYLNRGGIKADAPLVNIDAVIDAGVEAGFVYKAKSLSALAAAIAADN
                     GVMKAANLERAVAEYGAGIATGKDVLGKPLDRARRLGPVPATSEYYVAVTGAPYIYST
                     CGGLDVNEDMGVLRGDGSVIEGLYAVGNDSMGVLFTDRKGYANYGGVAEGYAFVSGRI
                     AGEAAAHK"
     gene            complement(9116..9979)
                     /locus_tag="PLQ29_00445"
     CDS             complement(9116..9979)
                     /locus_tag="PLQ29_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85139.1"
                     /translation="MGTKARGASAPIAWIVAALLLAPAVSASASPFSARATIDWTAGT
                     LRADVELDLMAAGIRLPSGRSQAERAMEEAVPGLIREAALAITLDSYRSVDDSIKDGT
                     LEPNAFEAFLDGGTRVQSALSRDLSRLTASYEWRLADLAALYVRHSVPIDLPEPARYA
                     PTKDYSGIVVYLSGAFAVRGEHREDRLRPCLFPRLYDEEMNAVIERNLLYPASLRERG
                     AVGYAVGLDDPVIAARAGDSPLRIIASSIFGSNRTDAVISADDALKILGSAANRELAR
                     QGKVVFVIDEP"
     gene            complement(9979..11376)
                     /locus_tag="PLQ29_00450"
     CDS             complement(9979..11376)
                     /locus_tag="PLQ29_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPG85140.1"
                     /translation="MRRPAVAAVAATLATAALAGRAFGLDGQAGTPTPSPAAAAIAAI
                     AAASEPEPPTSAAAAEQAIPRPLRSSGLGAPLYEHHAGPERYTLELPYGEPQFERFRA
                     SYLTAGGRLWLEAVYERSRPYASHVLERIRYYGLPEELFFLPFIESEYSAKAVSRSGA
                     SGLWQFMRNSVGGYDMRIDDWVDERRDFMKSTDGALRKLLSNYERFGDWPLAIAAYNC
                     GAGAMDRAIKAGGGVRDYWVLRERGLLPSETASYIPKFLAVVSVAMHGGRNGLAQSWT
                     PSIEWARVALDRPVDLAMLASASGTPLELLKAGNPELRYGVTPPEGRYAVKVPADYEP
                     AVRKALASRDVLMNVYLHTVASGDTVSALAKHYEVSVAMIAKMNPGLDPGRIRIGQRL
                     VIPAFKDKRPYVAAPKADDGAAFDGAYVVMKGDTLWAISLKYDVQPEILAARNGMSLD
                     AVLREGTSLIVPILE"
     gene            complement(11373..12428)
                     /locus_tag="PLQ29_00455"
     CDS             complement(11373..12428)
                     /locus_tag="PLQ29_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG85141.1"
                     /translation="MLRSPTLALCALCAAMCATLTACSSPRDYFIETPGADAGQLRDL
                     FELLDSGSLGVDERYAVIQKISSSLSDAKDYARLVDFLSEVVARDPEGQYNARHLLTI
                     AWAYSRQGADDIAELYYDRVLKNFPDLTVNGESIHFACLRRLIELSENSARRIEFRRE
                     LIARFPEKIDMGIALFLLGYDYESIGDWDAALDSYRRFLPASGAEVPGHPDAIQYAQQ
                     FIDLATIRKDWTYETLDELLEAVSTAMATGNARSLARIRAKVGFFAMDWYQDKSDGNS
                     QVLFDFAAFTSGTRIQRAPALDPSSNANEAFLRTWGWTERISVWYLYFRKVNFPADPE
                     VHGRWEWAGIYFGEKMQ"
BASE COUNT         2197 a         3968 c         4286 g         2037 t
ORIGIN      
        1 ggcgaccatc accgagctgg ccaacatgat caccgcccag gccgtgacca agctccatga
       61 gctgggtttc cgcttcgacc tgacgccgcc ggcgatattc accggggata acatggaagt
      121 ctcgaaccag gatgtggaag ccctcatcgt gccgatggag gcccctcagg gcaaggtcga
      181 gatcaacgtg gccatccgcg agcgggctta gggggctacg catgaagact aaagaggatt
      241 tcccgtccat caacgagcgg gtgcccgagg ggcagcgccc cgacggcacg cccttcaggg
      301 tactcgtcgt cgacgactcg atgttcgtcg cgaagcagat tacccagata ctgacctcgg
      361 agggctacga cgtcgtcggc caggccgctg acggcctcca gggcctcgag cgctataagg
      421 agctgttccc gaacgtcgac ctggtcacca tggacataac gatgccgaac atggacggca
      481 tcaccgcgct ccagaagatc atcgagttcg ataagaacgc ccgcgtgatc atgatcagcg
      541 ccctcggcaa gcaggatctg gtcaagcagg ctctgttgac cggcgccaag aactacatcg
      601 tcaagccgct cgatcgcaag aaggtgctcg agcgcatcca gcacgtgctg tcgaagtgat
      661 cgcctggcga tcggctggtt gacacgccgg ccgcggctct ggtataaact cccgtcgacg
      721 cgaggcgata gccgaaacgc cgataggttc gggcggttag ctcagttggt tagagcacca
      781 gtatgacacg ctgggggtca caagttcgac tcttgtaccg cccaaaaaac ccatccgtga
      841 ggatgggttt ttttgtccga gtcgcctgac ggtagcgccg ccaagccgag ctgggccgct
      901 acgcgaggcc cgcctctcgt agcagcgcca gcgcgtcgag gcgcggccgc ggtaagcccc
      961 ttggcaggaa agcggccagc ggcctatcgg tctcgccgtc gtcgatccag gcggcaagcg
     1021 acgcgtcgag cggggcggtc cagcgctcgg accgctcctc gccggcgaag cgcagatcgt
     1081 tgagcgcgaa ggagctgggc ggcgccagcg gctcgagccc gggccgctcg ccgcgctccc
     1141 aggccgcgta gatcgccgaa tggcgcgtca gcacgaagcg gtgccagaag gccgagtcga
     1201 cgagcccctc ggcgaggagg acccgcgcca tctcggccga gtcggctatg tcctgatcgg
     1261 actgcccggg gaagccgtag atcaggtagg cgtgcacgag caccccgcat cgtttgaagg
     1321 acgccagggt gcggaccagg tcgaccatcg agaaaccctt atcgactatc tccagaccct
     1381 tcttcgtggc tatctctatg cctccggaaa cggccacgag gccacgctcc gcggcgaagg
     1441 cgcaggactc gtcggtccag gcgcggtcga agcgggcgtt gccccagaac gtgaacggcc
     1501 tcgagcgcct cgcgttgcgc tcggcgaaag ctcggaccag attgaccggc atggcctcgt
     1561 cgacgaagtg gatgccatag aggccggtcc gcccggccgc cctcccggcc gcctcctcga
     1621 gcgcgtcgag gtcggaggcc aggtagcgct tgatgtaatc caactgggtg tcgcagaagg
     1681 cgcagcgccg ccagtaacag ccgtaggcca gccgatactt gagccatggc gtatcgttcc
     1741 agaggcggtg catcgggttc ggcgagtcga cgacgcgcag gtaaccggag acgtcgagcc
     1801 cggcgtaatc ggggtgggag ccggctatcg cctcgcgctc gagccgttcg tagccgctcg
     1861 ccttggacga gtcggccggc gccgaggctg ttccggccgg cgccggggcg gttccggccg
     1921 ctccggagga cgtcgcgcca gatcgaggct cgtcggcagg gaagccggcc gcgacgactc
     1981 ggccctcctc ttcgcggtag cggacgcggt atagcgacga acgtggcgct ccgtccacca
     2041 cgtcgaggat cgaggcgagg gcgccatagc ccgcgtcgta ggccaggtag tcgaacgcgt
     2101 cgaagggcgc ggggtcggtc aggaagcgta gctcggtcga gacgtagccg ccgccggcca
     2161 cgatggtagc ccggtcgccg aaggcggcct tggcctcctc ggcggcgacg acggcgccgg
     2221 ccagacagcc tgggaagggt acggtgacga gtacgagcgc gtcgtcgtcc gcgcggaggc
     2281 cttccttgaa cttggccatg gacgcgcgag cgatgggtcg gtagaactcg cgtaggaccc
     2341 agccttcatg ggcggctcgc cgtatcggcc cgaagtccgg agcgctggca gccaggcgct
     2401 cggcgtagcg caccgtgccg aaggacgggt ccagggcgta ggcgatgaaa tccgacaggt
     2461 cgttcagggt agccgtggcg aaggcccgcg cgtcgtccgg gccgatgccg tcgccgaaca
     2521 cggcctcggc gcgcatgccg gccggcagac ccgagccgac ggctatacgg taggcgtagg
     2581 cgggatcatc gcccgagagg aaggccgtca tcggctcgac gcaggccagg tacgccgcct
     2641 cgtcgtcgaa gtacgcgcct atccgcctgg ccgtctcgtc gtcggggtgt tgcctgcccc
     2701 ggagcgcgtc cctggccgcc gcgaacaccg tctcgaggcc ggcccggctg aagacccgcc
     2761 gcgccatggc tatcgagtgg tcctccgccc gcgagtcgac gccgcgcgag cgtagccacg
     2821 cgtcgaggta ccagactgcg gggtaggggg cgttgagctg gacgaggggc ggctggatgc
     2881 atatggctcg catgggagcg agtatagcgg ctcacgatat tcttcgggta gtccttgacg
     2941 cgatcatggg ttttttgtat aaatatgccc gcaccctagc cgctgtagct cagttggtag
     3001 agcagtggac tgaaaatcca cgtgtcgtca gttcaattct gaccggtggc acgaggcgcc
     3061 tcccgaatgg gaggcgtttt tttttccacg tcgtcgctgc ctcggtgctt gccgcgttcg
     3121 ccgttcccgt ctagtatcct agcggcggcc gcgccttttc ctggactggt ccggcgccgg
     3181 acaaggagac cgagacgtga aagtactggt tatcggaggc gcggggtaca ttggcagcca
     3241 cgtctgcctc gagatgatgg aggccgggca cgaggtcaca gtctacgaca acctgtcgag
     3301 cggcctacgg gagaacctgt tcgaaggcga gcgcttcatc cagggcgacg tcctggacgc
     3361 cgagggcctc gcgcgcgcga tgtccgaggg cttcgatggg gtcgtgcacc tggccgcgtt
     3421 caaggcggcc ggcgagtcga tggtggcccc cgaaaagtat gcgcgcaata acatcaacgg
     3481 caccatcaat atcctcaacg cgatgtgcgg cgccgggatc gggcgcttcg tcttctcaag
     3541 ctcggcggcc gtctacggcg agccgaccta cctgccggtg gacgagaagc acccgaccgt
     3601 cccggagaac ttctacggct tcacgaagct ggagatagag cgcatcctgg gctggtacga
     3661 caggctcaag ggcctcaggt actccgccct gcgctacttc aacgcggccg gctacgacgt
     3721 tcgcggcagg gcgcgcgggc tcgagcgtaa cccggccaac ctgctgccgg tgatcatgga
     3781 ggtcgccgcg ggcatgcggc ccgagctacg cgtgttcggg cgcgactacg atacgaagga
     3841 cggcaccggc gtgcgcgact acgtgcacgt gaacgacctg gcggtcgccc acgtggcggc
     3901 cctcgagcgc atggagcgta ccaatgagag cctcgtcgtc aacctcggct cggaggaggg
     3961 cctgtcggta ctcgagatac tcgaggcggc caggcgcatc accggaaagc cggtacccgc
     4021 gaccatcgag gggcgcaggc cgggcgatcc ggccaagctg gtcgccagct cggctctcgc
     4081 tgcggagaag ctcggctgga aggccaggta ctctgacgtc gacacccttg tcgagaccag
     4141 ctggaaggcc tacgagcgct gagcccgagg ggccggctaa cgaaaaaggg ggaaacgcat
     4201 ggactatatc aagcaggcca tcgactacct ggaccgcgcc gaggcgggcg tcatcgaact
     4261 cgagaagctc ctgacggcca tcccggccct ggcgccggaa tccggcggag acggcgagtg
     4321 gaagaaggcc gaggccctcg aggcctggct acgcgcccgc ggcatcgtcg acatagagcg
     4381 ccatgacgcg ccggacgcca gggtgagcgc cggcaaacgt ccgaacctgg tcgccaccat
     4441 caagggcagg aagccaggca aacgcctctg ggtgatggcc cacaccgatg tggtgcccga
     4501 gggtgaccgc tcgctctggg ataccgagcc tttcgaggcc gtcgtgaagg acggcaagat
     4561 ctacggccgc ggcgtcgagg ataaccagca gggcctggtt tcgagcgtgt tcgcggcgct
     4621 ggctctcgtg gagaacggta tcgcccccga gcgcgacgtc aagctcctgt tcgtcgcgga
     4681 cgaggagtac ggctcggtct acggcatcca gtggctgctc gagaaccgcg agctgttctc
     4741 gccggacgac ctgatcgtcg taccggactc cggctcggcc gacggctccg agatcgagat
     4801 agccgagaag aacgtgtgct ggctcaagct ggcgaccaag ggaaagcagt gccacgcctc
     4861 caggcccgac gacggcgcca acgccttcct cgcgaactgc gaactggccc tgagcctgaa
     4921 cgcgatggag cgcgaatcct tccccgcgac cgaccctatc ttcgtgccgg accgcacgac
     4981 gattacgccg accaagaagg acgccaacgt gccgaacatc aacacggtgc cggccgacga
     5041 cgcgttctac gtggacatgc gcatcctgcc gcgctactcg atcgacgagg tgctcgccga
     5101 gacagccaag cgggcggccg ccgtcgagcg gaagtacggc gtcaaggtcg gcgtcgaggt
     5161 ggtgcagcga aacgatagcc cgccgactcc ggccgacgcg cccgtcgtca ccctgctcaa
     5221 gcgcgccatc gaggagacct acggcgtggc cggcagggcc gtcggcgtgg gcggcggtac
     5281 cgtcggcgcc tacctacgct cggcgggttt cccctgcgtg gtctggagcc gccaggacga
     5341 gacgatgcac gcgccgaacg agtacacgag gatcagcaac ctgatcggcg acgccaagat
     5401 cttcgcccgc ctgatgctcg ccgagcgcta agccggtacg ccgggccggt acgcataagg
     5461 cgtagaagag gataagaaaa taagagcaaa gaaagaggaa atgtaaaaga ttaagagact
     5521 gataaagggg gcgggagggg ctaatacctc ttcaccaccc cctcttttcc gcgtaatcct
     5581 cttgaatccc ctcgtgtcct cttctgctct tcgcggattt tcggtatcgt cgatgacgaa
     5641 ggcgaccgtg cccctgcggg ccagctcgcg ccgatggatg ggcgcctaga ccgcccatcg
     5701 cctgaaccga cggtccgtat cgaccgaccc taagcttctc actaggcgcg gtcatggttt
     5761 tcctgcatta ttcgctacca tgaagagcgc catgctattc ggggctaagg agctacggcg
     5821 gctcacgggc atcagcgccg agacccttcg ccactatgtc gaccgcgacg tcctcaggcc
     5881 gctccgcgtc gacgggaaca attaccgcaa attctccagc cagaacatga tagacgtgct
     5941 ccacgcccgt atctaccggg gtttgggcct gcccatccaa agcatcaccc ggaagagcgg
     6001 ctccgggccg gaggggcagg acgacgtgct ggcgcgccat caggccatcc tcgagcggga
     6061 ggccctcgag ctgcagatga agctcgcccg tatgcggcag gagttggagt tcctcgccac
     6121 ggctcgtcgc aggatcggat gcgtccgggt caggacagcg gacgaggcgc cgtcggtcta
     6181 tcgcctgacc ctgctcgacg cggccgacgg catgataagc gacgccgagg tcggaaggcg
     6241 cgtcgccgaa tggatgcgct acgcccctca cctccacgtc gcgctacggg tgcctgaggc
     6301 cgccgtactc gatccgagcg tcgaggagct tccggtctcc ctcgggctca gcgtgcgctc
     6361 ggagacggcg tcgtccttag ggatgaacgc cggcccgccg gcggagtact gcccgaacaa
     6421 ggctcacgtc ggtaccctcc tggctttggc cgacccgttg agcctacggc ggcgggacat
     6481 cctacccctc ctggataccg tccgggaacg cgggctcagg ctcgagggcg gcctgatcgg
     6541 caggctgtgc tatatcgtcg acgcggaagg gggacgactc tattacttct cggtcaacct
     6601 ctgtgtagtt tccgcttgac cgtgtggtta ccacgcgcat tacgatccca ttcaggcgga
     6661 tagcctcagt accgttataa ggggatccat gatgaagatg cgaaggacgt atcggacgct
     6721 ggtcgcggcg atcgcgatat tggtttcggt cctggccgga tgcaatagcg tcgggcgtgg
     6781 gggctcggta tacgccccgg gcacgtactc cgtgtcgacc aagggcttcg gcggtgacgt
     6841 gaccgtggcg ctcacggtcg acgccaggtc catcgtcggc gtggagatag tcggcgagga
     6901 tgagacgcca gagatcggcg gcatggccat ggagcgtatg gcccgcgata tccctgccgt
     6961 cggcgaggcg gtcgagggcg tcagtggcgc cacgtacacg agcgacgccg tcaagcgggc
     7021 gctctccgag gctctggcgc gggcccgcgg cgagagcgtt tccgtcaagt tggacgtcgc
     7081 gccgggtagc tatggcgcgt cggcgccggg cttcgacctc tccgtcccgg tgcccgtgac
     7141 ggtcggcgtc gtggacggcc ggatcgcttc gatagagata ggcgcgtgca aggagaccga
     7201 gggtaagctc accgcggtgc gcgaccgcct gataccgcgc atcatcgagc ggcaatcgtt
     7261 agccgtcgac gccgtcaccg gggccacggc cacgtccaac gcggtcatcg gagccgtacg
     7321 ggctagcctc gtcgcggccg gagcgtccga ggcggccctg tacgcccccg tccccaggtc
     7381 ctcggccaag gaagcctata ccgccgatgt cgtcgtggtc gggttcggcg cttccggcat
     7441 gacggcctcg ctcgccgccg ccgagaaggg cgccaaggtc atagccctcg agaagagcgg
     7501 tcggattggc ggcacggccg tggtgaccag cggcccgatg gcggtcaacg ctccgagcat
     7561 ggtcgggaat aggatcgtcg actgggacga tccgttactg aagaagaagc gggtcaagga
     7621 agccggcgag ccgctggtcg acgcgggggc cctgctacgc gattggctcc agtacacgac
     7681 cgtcgacggc gttcagggcg ccaaggaagg gatcatacgg cgggtcgtcg accgatcagg
     7741 ccagaccctg gactggctga ccggcttcgg cttcgagttc tccgacgcga ggggcttcct
     7801 ggggaaccag tgggcgctgt tctccccctt caagggcaac aaggccctga ccgagagctt
     7861 cttcgacaag gccgtggcac ggttcgagaa agagttcggc ggccgttgcc tggtagagac
     7921 cgaggcgacc tctctcatta tcgagggcgg ccgcatcgcg ggcgtccggg ccaggaagtc
     7981 cgatgggacc gaggttaccg ttaaggccaa ggcggtcatc ttggcgagcg gcgggttcgg
     8041 cggcagcgac gagatgatgg agcagtacct cggcgagtcg tggaagatgt acggcatgga
     8101 gcagaacgag ggagatggca tacgcatggc cctgagcgcc ggcgcggcta cctacaacat
     8161 cggtatgccg ccgatgtcgc acttctcggc cccgtcggtc atcctcagga gcttcgactc
     8221 cgacttcgac aacgacatcc cctttgggat ggtggccacc agcgagaccc tggcggtgaa
     8281 caagtcgggc gttcgcttca tcaacgaggc caatatcggc ctcgatgcct acctgggcgg
     8341 ctcgcgcttc tacacgatct attccaagga gcagatcgat gtcctcagag ccaggggctt
     8401 cggcttcgcg gcgaccgggc gctacctgaa tcgcggcggt atcaaagccg acgcgccgct
     8461 agtcaacatc gatgccgtca tcgacgccgg cgtcgaggcg ggctttgtgt acaaggcgaa
     8521 gagcctctcg gccctggccg cggccatcgc ggccgacaac ggggtgatga aggccgctaa
     8581 cctggagcgg gccgtcgcgg agtatggtgc cggtatcgcg accgggaagg acgtcctggg
     8641 caagcccctg gatcgagcgc gccgcctcgg cccggtaccc gccaccagcg agtactatgt
     8701 cgccgtcacg ggcgccccgt atatctacag cacctgcggc ggtctcgacg tgaacgagga
     8761 catgggcgtc ctcaggggag acggctcggt catcgaaggg ctctacgccg tcggtaacga
     8821 ctcgatgggc gtactcttca ccgaccgtaa gggctacgcc aactacggcg gcgtggccga
     8881 aggctacgcc ttcgtctcag gccgcatagc cggcgaggct gccgcccata agtaagcgcc
     8941 actcctagag taggataacg aagaggatat aagaataata gcaacgatag cgaaccctga
     9001 ctcaggttaa gagaagcgag taaagggggc ggagagacta tcagctctcc gcccccttcc
     9061 tatcttcact tcccgcttga gttctctcgt atactcttta ttactcttac gataatcagg
     9121 gttcgtcgat gacgaagacg accttgcctt gcctcgccag ttctcggttg gccgccgagc
     9181 cgaggatctt gagcgcgtcg tcggcgctga tcacggcatc cgtacggttc gagccgaaga
     9241 tagacgaggc gatgatgcgg agtggcgagt cgccggccct ggccgctatc accgggtcgt
     9301 cgagcccgac ggcgtagcct acggcgccgc gctcgcggag cgaggcgggg tagaggaggt
     9361 tgcgctcgat gacggcgttc atctcctcgt cgtagagtcg agggaagagg caaggcctca
     9421 ggcgatcctc tcgatgctcg cccctgacgg cgaaggcccc gctcaggtag acgacgatgc
     9481 ccgagtagtc cttggtaggc gcgtagcggg ccggctctgg caggtctatc gggacgctgt
     9541 gcctgacgta gagggccgcg aggtcggcga gccgccactc gtacgacgcc gtcagccggc
     9601 tcaggtcgcg gctcagcgct gactgtacgc gcgtgccgcc gtccaggaag gcctcgaacg
     9661 cgttcggctc gagggtgccg tccttgatgc tgtcgtcgac gctacggtac gagtccaggg
     9721 taatggcgag ggcggcctcc cgtatgaggc ctggcacggc ctcttccatg gcgcgctcgg
     9781 cctgcgacct gccggacggt agccgtatgc ccgccgccat caggtcgagc tcgacgtccg
     9841 ctcgcaacgt acccgcggtc cagtctatcg tcgccctagc gctgaacggg gacgcggaag
     9901 ccgagacggc gggcgccagg agtagggccg cgacgatcca ggctatcggc gccgaggcgc
     9961 ctcgtgcttt ggtacccatc actctagtat cggcacgata agcgacgtac cttcgcgcaa
    10021 cacggcatcg aggctcatgc cgttcctggc cgcgaggatc tccggctgta cgtcgtactt
    10081 gagcgatatg gcccagaggg tgtcgccctt catgacgacg taggctccgt cgaaggcggc
    10141 cccgtcgtcg gctttgggcg cggcgacgta ggggcgctta tccttgaagg ccgggatgac
    10201 gaggcgctgg cctatcctga tgcggcccgg gtcgagcccg gggttcatct tggctatcat
    10261 cgccacggag acctcgtagt gcttggccag cgccgagacg gtatcgccgg aggcgaccgt
    10321 gtgcaggtag acgttcatca gtacgtcgcg cgacgcgagg gcctttcgga cggccggctc
    10381 gtagtccgcg ggtaccttga ccgcgtagcg accctcgggc ggagtgacgc cgtagcgtag
    10441 ctcgggattg ccagccttga gcagctcgag gggagttccc gaggcggacg cgagcatggc
    10501 gaggtcgacc ggccggtcga gcgctacccg ggcccactcg atcgacggcg tccaggactg
    10561 cgcgaggccg ttgcggccgc cgtgcatggc caccgagacc acggccagga acttgggaat
    10621 gtacgaggcc gtctccgacg ggaggaggcc tcgttcccgg agaacccagt agtcgcggac
    10681 gccgccgccc gccttgatcg cgcggtccat cgcaccggcc ccgcagttgt acgccgctat
    10741 agccagcggc cagtcgccga agcgctcgta gttcgacagg agtttacgca gggcgccgtc
    10801 ggtggatttc atgaagtcgc ggcgctcgtc gacccagtcg tcgatacgca tatcgtagcc
    10861 gccgacgctg ttgcgcatga actgccagag cccggacgcg ccggagcggc tgacggcctt
    10921 ggccgagtac tccgactcga tgaacggcag gaagaacagc tcctcgggca ggccgtagta
    10981 gcgtatccgc tcgagcacgt gcgaggcgta gggcctcgag cgctcgtaga cggcttcgag
    11041 ccagagccgg ccgccggccg tcaggtacga ggccctgaag cgctcgaact gcggctcgcc
    11101 gtacggcagc tcgagcgtat agcgctcggg gccggcgtgg tgctcgtata ggggcgcgcc
    11161 gaggccggag gatcggagcg gcctcgggat cgcctgttcg gccgcggccg cggacgtcgg
    11221 cggctcaggc tcggaggcgg ccgcgatggc ggcgatggcc gccgccgccg gggaaggggt
    11281 cggcgtcccg gcctgaccgt ctaggccgaa ggcccgcccc gcgagcgcgg cggtcgccag
    11341 ggtcgcggcg acggccgcga cggccgggcg tctcattgca tcttctcccc gaagtagatg
    11401 cctgcccact cccagcggcc gtgcacctcc gggtcggcgg gaaaattcac cttgcggaag
    11461 tacaggtacc agaccgagat acgctcggtc catccccagg tgcgtaggaa ggcctcgttg
    11521 gcgttcgagc tcgggtcgag ggctggagcc cgctggatgc gcgtaccgga ggtgaaagcc
    11581 gcgaaatcga acagcacctg gctgttgccg tcggacttat cctggtacca gtccatagcg
    11641 aagaagccga ccttggcgcg tatcctggcg agcgatcgcg cgttgcccgt cgccatggcc
    11701 gtcgagacgg cctcgagcag ctcgtcgagt gtctcgtagg tccagtcctt gcgtatggtc
    11761 gccaggtcga tgaactgctg ggcgtactgg atcgcgtcgg ggtggccggg tacctccgcg
    11821 cccgacgcgg gaaggaagcg tcggtacgag tcgagggccg cgtcccagtc tccgatggac
    11881 tcgtagtcat agccgagcag gaacagcgct atacccatat ctatcttttc gggaaatctg
    11941 gcgatgagct cgcgcctgaa ttcgatgcgc ctcgccgaat tctccgataa ttctatcagg
    12001 cggcgcaggc aggcgaagtg gatggactcc ccgttgaccg tcaggtcggg aaaattcttg
    12061 agtacccggt cgtagtacag ctccgcaata tcgtcggcgc cctgcctcga gtaggcccag
    12121 gctatggtca ggagatgcct ggcgttgtac tgcccctccg ggtcgcgcgc gaccacctcg
    12181 gataggaagt cgacgagccg cgcgtagtcc ttggcgtcgg acaacgacga ggatatcttc
    12241 tggatgacgg cgtagcgctc gtcgacgccg aggcttcccg agtcgagcag ctcgaacagg
    12301 tcgcggagct ggccggcgtc cgctcccggc gtctctatga agtagtccct gggtgacgag
    12361 caggccgtga gggtcgcgca catcgccgcg cagagcgcgc agagggcgag cgtcggggaa
    12421 cgaagcatgc cggactatac cacgcgctct tttgcgttgg caaggaatcg ctttgtggat
    12481 acactaga
//
LOCUS       DAOPHV010000008        12474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_6000_length_12474_cov_4.131251, whole genome shotgun sequence.
ACCESSION   DAOPHV010000008 DAOPHV010000000
VERSION     DAOPHV010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 12474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12474
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6000_length_12474_cov_4.131251"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLQ29_00460"
     CDS             complement(<1..323)
                     /locus_tag="PLQ29_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG85142.1"
                     /translation="MSKLSTTGLTKAFSGLVAVNDVSFAMEEGEILGIIGPNGAGKTT
                     FIHLVSGILMPSSGRIEFKGRDVTYLPAHERSRLGIARTYQLIHPLENLKVLENVMVG
                     AVFSRG"
     gene            complement(320..1300)
                     /locus_tag="PLQ29_00465"
     CDS             complement(320..1300)
                     /locus_tag="PLQ29_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPG85143.1"
                     /translation="MKIRGAYIAAAVAAAIMIAFPAFLGAYPLNVARSVMTYMALAVS
                     WDMLLRSGQISFGIAGLFGLGSYASILAVLRGGMDPLLSIAFAALVAGLVAFALGFAI
                     LRLRAMYFSITTLALMEIFRIIMHNLHDFSGGPEGVVLPEVIFGGDASALYWLCLGGL
                     AVTIGASWYFERSKLRLALTAIRNDETSARSNGIDIFKYLLVAFVVTSTLQGMMGAIQ
                     VQSYGFTTPDTAFDANFTLLPLAMALLGGIHSTIGPMAGAVLLGIASEWLKLKIPYGH
                     LVVYGVIIIIVILFMPNGLFGLARKAIRKAGGAGSPGSASNGSDAEEGAR"
     gene            complement(1297..2220)
                     /locus_tag="PLQ29_00470"
     CDS             complement(1297..2220)
                     /locus_tag="PLQ29_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPG85144.1"
                     /translation="MVNKRKTTLVVALIAAAAVIILAWRPTVLIYGLQAAGLYASIAV
                     PMGLILGIVHIVNLAHGEFLMVAAYATYLLCKSLGIDPYLALLPVTAVTAGLGFVVYK
                     LTIRQTLKAPELNQLILTFGLGISMTQIANLLFTSQTRKLSFDYVSASIDIGELSFGV
                     YNFVFLGAALLMVVGLRWYLTRTTAGKAAVAVGQNPRGAAIVGIDVDRTYGFVFAMAT
                     ALVGMMGAMLITRSAVFPTVGSPYTMKSFCLVAMAGIGNIPGILGASILLGVSENLLK
                     AFRGLRGWADIVFFVLIITVILGRSIRGKKA"
     gene            complement(2299..3504)
                     /locus_tag="PLQ29_00475"
     CDS             complement(2299..3504)
                     /locus_tag="PLQ29_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPG85145.1"
                     /translation="MKKALAILMVACLAVPAFAQSGGTIKLGGTWPLGDITGKQGSMA
                     AQQAVDEINAAGGVLGRKLELIVIDDELKADKGAAAVEKLVTVDGVDALIGGMASGVA
                     LGQIPAMKKYGKIVIATGAASYKVEEALGPDANWYFHLHPWDYNQGASYAQGWDEIQK
                     KYPAVKIRKIFLAYEEGAFGKSSFDATKVLFGADKRYSIDGASFKSAALGGGDYGAVL
                     EAAKEFKPDLFLWAGYDADALPMLEQAKAMKFTPGIYLGAPPGWPVDFGASKLSDNVM
                     LYGMWAPSINDVSKVSKAFYNGFVAKYKVEPATYFAPLSYSAVYIYADAVKRAGTTET
                     AAVIKALEATKYASPLGETITFTPSNIIKHQGIKNQKILQWQNGRQEVIWPFEAATAK
                     PVYPFPKWK"
     gene            complement(3599..4339)
                     /locus_tag="PLQ29_00480"
     CDS             complement(3599..4339)
                     /locus_tag="PLQ29_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPG85146.1"
                     /translation="MDRMKGKVCVVTGGARGIGRGIVETFAREGALRVWALDMNEAEL
                     AALERDIPCVKGEVVNVADGAAVEAFVAKAKAEYGRIDVLVNNAGITRDALIQKMTES
                     DWDAVINVNLKGPFLMTKYVAPVMMEGGSGSIVSISSVVGLDGNIGQTNYAATKGGVV
                     AMTKGWAREFSRKGAKIRANCVSPGYTRTPMIETVPEKVLEPIIARTPLGRLAEIQDI
                     ANGVLFLSCDEGSFVNGQVLRVDGGLTL"
     gene            complement(4385..5404)
                     /locus_tag="PLQ29_00485"
     CDS             complement(4385..5404)
                     /locus_tag="PLQ29_00485"
                     /inference="COORDINATES: protein motif:HMM:NF005308.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HPG85147.1"
                     /translation="MMHIGVIGIGTYVPDTFLSAADLAKATGVPEAVVAEKFGVRRKP
                     VAGPDDTTAVMGLRAARAALADAGVEGKDLGLVLWCGAQHKDYPCWLAGLYVANELGA
                     SGAWSFDMEAMCGSMMAALDVAKSLMLARDDLDTVLLVSGYRNNDLIDLSEPTTRWMM
                     DIGSGGSACVLRKNSGRNVILGSSFRGDGSMSLDCVVPTLGSKAWPPRPGDLERAHFS
                     IPDEAGFKAKLGERTMPNFYAVIDRALELSGGLGRADIGYLAMLHFKRSAHDGILAEL
                     GLDESRSTYLDQYGHLGQNDQLLSIRLGLGDGRIRDGDYVVLVGAGLGFVWAASVVRW
                     GPWIE"
     gene            5608..6762
                     /locus_tag="PLQ29_00490"
     CDS             5608..6762
                     /locus_tag="PLQ29_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPG85148.1"
                     /translation="MVQRGADTQRKLVETAIALFASRWYSSISVAEICRAAELSNGVF
                     YRYFPNKEAIFFHILDDVIVRIAAALERTEGGAIEERIRSMTEILVSFSAEHPDLITV
                     FREGQYRYFEYERRLTETYRRILSKTLGRPAGVAEYLVGIGGPRFAAVRSALQGANIS
                     TAALADMAANGLFPGLDWDPERVFGITIAPPAIRLEESSRDRLMKAGKRLFGERGFHA
                     VNIHEVTDAAGLSVGAFYKHFDSKESFFREQIASAGKEVRHFIASNLTPGLNRLEEEM
                     QGMFLFGVYLSLDRWCYNIAREGEFVALPTVKEYYAAFKRGYERLGAAGMDQEAVASD
                     PLYLDSAAEFMMGLSHYYGLEVAFDESPHNARAIVEGVGEYLSHGLARRS"
     gene            6783..7793
                     /locus_tag="PLQ29_00495"
     CDS             6783..7793
                     /locus_tag="PLQ29_00495"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HPG85149.1"
                     /translation="MAKAKILGTGVYAPGAPIGNGELARLAGIELDAARLEEKIGIRA
                     RHIARLRGLDESTADFAERAARSAIADAGLAPDDLDLVIVGTDTPEYVSPATAILVQG
                     RLQGGQRDTAAFDVSASCASFVTALDAAARMMAGDPALRHAVVVGVYNMPAHVRNGDA
                     FGWSIFADGAGAVVLSRTDDDDPSGYITGKFIADGTQWDYVGVYAGGTRKPVTRELLD
                     AGAYGLENLKRLPGDRNLKLWPPVIRALCDKGGVGLEDVAGFVMTQINRSVILEVMDE
                     LGRPRELAPLAMDRYGYTGSACVPMAFHHAIKDGRIRRGDPIVFCASGAGLAVGANLF
                     TY"
     gene            7861..9171
                     /locus_tag="PLQ29_00500"
     CDS             7861..9171
                     /locus_tag="PLQ29_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HPG85150.1"
                     /translation="MSRWDDEYRSRLRPIDEAIQCIESGYDVVVGQCASEPQGTMARL
                     HILAPRVRDVRVFSVLTLKAYDFYLKPEMQGHFELASWFHAPGSRAALKAGTGTVTFV
                     PNMLHRAATDRIHARPPEVFIGTCTPPDHNGFVSLSLGITYEKDILEAARYVILEVNP
                     RLPRTLGDTHLHVSQVTYFVEHEQEVPSLPAPEPSETDLLIGRYIADLVEDGSTIQLG
                     IGGIPNAAALALRGKKDLGVHTEMLVDSMMELYEMGVITNRRKSLRKDKFVATFAMGS
                     RKLYDWLDDNVAVDFERGRWVNDPAVVARNSRMVSINTCLTVDFTGQVASESIGAQQY
                     SGTGGQSDTAQGAVAGFDGLGKSIIACYSTAKGGSLSTIVPTLPEGSAVTLHRSFVDH
                     VVTEHGVAKLRGRTVRERALALIAVAAPEFRDELKSQAKRLGYI"
     gene            9206..10462
                     /locus_tag="PLQ29_00505"
     CDS             9206..10462
                     /locus_tag="PLQ29_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85151.1"
                     /translation="MSKVSIVGAYNTRFGNLVKKDKETGAVSDLASLYELMLEAGQGA
                     LTDAGVQADEIGGVWVGSCAPGLFANQEHVAAFAPEIDPAGLRFKPMTRCEDACASGS
                     VALYDALYAVEAGRVGAALVVGVEKMNLLDTGGVTHALATCSYWPEEGARGMTFPSLF
                     AEYAKGYRDRYGLDDAALARMLASVAALCYRNGLENPLAHFGKGGPSDRLGLVSAEAI
                     LALPPEKNPMIAEPLRLHDCSLVTDGAAAVVIVGPGHPAVKRGRAVRIAGVGHANERI
                     AISKRKNMYELQAAKRATALAFAEAGIGSGDVDIAEVHDCFTINQLLCTEALGLSVDG
                     RAGYDYLDGRFGPDDEAAVNLSGGLKAKGHPVGATGVSMHALVYKQLVGEAIGLAPSK
                     TPELGVTLNVGGSAATNCVTVLRRER"
     gene            complement(10492..11598)
                     /locus_tag="PLQ29_00510"
     CDS             complement(10492..11598)
                     /locus_tag="PLQ29_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HPG85152.1"
                     /translation="MTRDAGPTDSIVDVAGVSVGHATIADGGLQTGVTAVLPHAGNLF
                     RDKAPAACRVFNGFGKSVGLLQVEELGVVESPILLSNTFAMSACVDGALDLLLAENPD
                     IARETGSANVVALECNDGYLSDMRRRAVTREHVAEAIRLARSGSFAREASLRIGAGGP
                     SAGAFARGSVGAGRGMSCYGLKGGIGTASRVALADGRPYTVGTLTLTNFGRLSDFVVH
                     GRLLGKEIEALGGPAAARIRAAAIDGSGVGSPGDGSVIVVLTTDAPLDSRQLRRLCMR
                     AASGLARTGAVIGGGSGELALAFSTAYTVPHDPPPGGGPLPRATLHEDLLDPLFRAAT
                     DCVEAAVLDSLYSAETVVGRDGHERVALSELGLG"
     gene            complement(11595..>12474)
                     /locus_tag="PLQ29_00515"
     CDS             complement(11595..>12474)
                     /locus_tag="PLQ29_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPG85153.1"
                     /translation="SLRRHFNVIGRLTGKGTYFFDYGNSFLKAVYDAGVREVSKNGVD
                     EKDGFVFPSYVEDILGPELFDYGYGPFRWVCLSGKDEDLRKTDAAAMACIDPNRRGQD
                     RDNWAWIRDAEKNRLVVGTKARILYQDAIGRTKIALEFNHMVKKGEIGPVMLGRDHHD
                     CGGTDSPFRETSNIKDGSNVMADMAVQNFAGDAARGMSLVALHNGGGVGIGKSVNGGF
                     GLVLDGSERVDAIISMAMPWDVMGGVARRSWARNEHAVATCAEYNEVYSGSGSITLPY
                     IADDRIVESALGGSGS"
BASE COUNT         2149 a         4175 c         4091 g         2059 t
ORIGIN      
        1 cctcgactga agacggcgcc gaccatcacg ttctcgagca ccttgaggtt ctcgagcggg
       61 tggatcagct gataggtcct ggctatgccc aagcggctac gctcgtgggc cggcaggtag
      121 gtgacgtcgc ggcccttgaa ctcgatccgc ccggagctcg gcatcaggat gccggacacc
      181 aggtgaatga aggtcgtctt gccggcgccg ttggggccga tgatgccgag gatctcgccc
      241 tcctccatcg cgaagctcac gtcgttgacg gccacgaggc cggagaaggc cttggtcagg
      301 ccggtcgtac tcagcttgct catcgcgcgc cctcctcggc gtccgacccg ttcgaggccg
      361 agcccgggct gcccgccccg ccggccttgc gtatcgcctt ccgggccagg ccgaaaaggc
      421 cgttcggcat gaataggatg acgatgatga tgatcacgcc gtagacgacc aggtggccgt
      481 acgggatctt gagcttgagc cattcggacg cgatgccgag caacacggcg ccggccatcg
      541 gccctatcgt gctgtggatg ccgccgagca gggccatcgc gagcggcagc agcgtgaagt
      601 tggcgtcgaa ggccgtgtcc ggcgtcgtga agccgtagga ctgcacctgg atcgcgccca
      661 tcatcccctg cagggtcgag gtgacgacga aggcgacgag caggtacttg aagatgtcga
      721 taccgttcga ccgcgcactc gtctcgtcgt tgcggatagc ggtcagcgcc aggcgcagct
      781 tgctcctctc gaagtaccag gacgcgccta tcgtgacggc caggcctccg aggcacagcc
      841 agtagagggc ggacgcgtct ccgccgaaga tcacctccgg cagcacgacg ccctcgggcc
      901 cgcccgagaa atcgtgcagg ttgtgcatga ttatcctgaa gatctccatc agcgcgagcg
      961 tggtgatgga gaagtacatg gcgcgcagcc tgaggatcgc gaagccgagg gcgaaggcca
     1021 cgagcccggc gaccagcgcc gcgaaggcta tgctcaggag cgggtccatg ccgccgcgga
     1081 gcacggccag gatggacgcg taggaaccga ggccgaacag gccggctatg ccgaacgata
     1141 tctgccccga gcgcagtagc atgtcccagc tgacggccaa tgccatgtag gtcatcacgc
     1201 tgcgcgcgac gttcagcggg taggcgccca ggaaggccgg gaaggctatc atgatggcgg
     1261 ccgctacggc cgccgcaata tacgcgcctc gtatcttcat gccttcttcc ccctgatcga
     1321 gcgtccgagt atcaccgtga tgatcaggac gaagaatacg atatcggccc agccgcgcag
     1381 gccgcggaag gccttgagca ggttctccga cacgccgagc aggatgctgg cgccgagtat
     1441 gcccggtatg ttgcctatgc ccgccatcgc gacgaggcag aagctcttca tcgtgtacgg
     1501 gctgcccacc gtcgggaaca cggccgagcg agtgatgagc atcgcgccca tcatgccgac
     1561 gagggcggtg gccatcgcga agacgaagcc gtaggtcctg tcgacgtcga tgccgacgat
     1621 ggccgccccg cgggggttct gcccgacggc cacggcggcc ttcccggccg tcgtcctcgt
     1681 caggtaccac cgcagcccga ccaccatgag cagcgcagcg ccgaggaaca cgaagttgta
     1741 gacgccgaac gacagctccc cgatatcgat cgacgccgag acatagtcga acgacagctt
     1801 gcgggtctgc gacgtgaaaa gcaggtttgc tatctgggtc atcgatatgc ccagcccgaa
     1861 ggtcagtatc agctggttca gctcgggagc cttgagcgtc tggcgtatgg tcagcttgta
     1921 gacgacgaag ccgaggcccg ccgtcaccgc agtcaccggc aacagcgcca ggtaggggtc
     1981 gatgcccagc gacttgcaca gcaggtaggt cgcgtaggcc gccaccatca ggaactcgcc
     2041 gtgcgccagg ttgactatat gcacgatccc gaggatcaac cccatcggta cggcgatgga
     2101 ggcgtagaga cccgccgcct ggaggccgta gatgagtacg gtcgggcgcc aggcgagtat
     2161 aatcaccgcg gccgccgcta tcagcgcgac gaccagggtc gtcttccgtt tgttcaccat
     2221 ctctatacct ttggtcgatg atagtccgcc ggcccttacg ggccggcggc cttggttcag
     2281 tacccgatcg tcacccggtc acttccactt cgggaaggga tacacgggct tggcagtcgc
     2341 ggcctcgaac ggccagatga cctcctggcg cccgttctgc cactgcagga tcttttggtt
     2401 cttgatgccc tggtgcttga tgatgttcga cggcgtgaag gtgatcgtct cgccgagagg
     2461 cgaggcgtac ttcgtggcct cgagcgcctt gatcaccgcg gcggtctcgg tcgtaccggc
     2521 gcgcttgacc gcgtcggcat agatgtagac tgccgagtac gacagcgggg cgaagtacgt
     2581 ggccggctcg accttgtact tggccacgaa gccgttgtag aaggccttgc tgaccttgct
     2641 gacgtcattg atcgacggag cccacatacc gtagagcatc acgttatcgg acagcttcga
     2701 cgcgccgaag tcgaccggcc atccgggagg cgctccgagg tagataccgg gggtgaactt
     2761 catcgccttg gcctgctcga gcatcggcag ggcgtcggcg tcgtagccgg cccagaggaa
     2821 gaggtccggc ttgaactcct tggcggcctc gagcacggcg ccatagtcgc cgccgccgag
     2881 ggcggcgctc ttgaaggagg cgccgtctat cgagtagcgc ttgtcggcgc cgaaaagcac
     2941 cttggtggcg tcgaaggacg acttaccgaa ggcgccttcc tcgtacgcga ggaagatctt
     3001 cctgatcttg accgccgggt acttcttctg gatctcgtcc cagccctgcg cgtagctagc
     3061 gccctggttg tagtcccagg gatgcaggtg gaagtaccaa ttggcgtcgg ggccgagggc
     3121 ctcctcgacc ttgtacgagg ccgcgccggt cgcgatgacg atcttgccgt acttcttcat
     3181 cgcgggtatc tggccgagcg cgacgccgga ggccatgccg ccgataaggg cgtcgacgcc
     3241 gtccaccgtt acgagcttct cgacggccgc ggcgcccttg tcggccttta gctcgtcgtc
     3301 gatgacgatc agctccagtt tgcggccgag cacaccgccg gcggcgttga tctcgtccac
     3361 ggcctgctgg gcggccatgc tgccctgctt gccggtgatg tcgccgagcg gccaggtgcc
     3421 gccgagcttg atggtcccgc ccgactgggc gaaggcgggg acggcgaggc aggcgaccat
     3481 gaggatcgcg agcgctttct tcattactgt cctccttgaa cctgacggac gcctacgcgc
     3541 ccgaatctat acgtgacggg ccgcggctgc cgtgccggcg cgcccctcga agccttggct
     3601 acagggtcaa tcccccgtcg acgcggagca cctgcccgtt gacgaaggaa ccctcgtcac
     3661 aggataggaa cagcacgccg ttggcgatgt cctggatctc ggcgagcctg ccgagcggag
     3721 tacgcgcgat gatcggctcg agcaccttct cgggaaccgt ctcgatcatc ggggtacggg
     3781 tatagccggg cgacacgcag ttggcccgga tcttggcgcc cttgcgcgag aactcccggg
     3841 cccagccctt ggtcatcgcg acgacgccgc ccttcgtcgc ggcgtagttg gtctgcccga
     3901 tgttgccgtc gagcccgacg acgctcgata tggagacgat ggagccggac ccgccctcca
     3961 tcatcacggg ggccacgtac ttggtcatca ggaacggccc cttcaggttg acgttgatga
     4021 cggcgtccca gtcggactcg gtcatcttct ggatcagcgc gtcccgggtg attccggcgt
     4081 tgttgaccag cacgtcgatg cggccatact cggccttggc cttggcgacg aaggcctcga
     4141 cagcggctcc gtcggccacg ttgacgacct cgcccttgac gcagggaatg tcgcgctcga
     4201 gcgcggctag ttcggcctcg ttcatatcca gggcccatac gcgcagcgcg ccctcgcggg
     4261 cgaaggtctc gacgatgccc ctgcctatgc cccgagcgcc gcccgttacg acgcaaacct
     4321 tacccttcat cctatccatg atcgtgctcc ttatatgcgt ccgccggcgc acggcggcgc
     4381 ggcattattc gatccaaggc ccccagcgga cgacgctggc cgcccatacg aaaccgagcc
     4441 ccgcgcctac gagcacgacg tagtcgccgt cgcgtatccg cccatcgccg aggccgagcc
     4501 gaatcgacag cagctggtcg ttctggccca ggtggccgta ctggtccagg taggtcgacc
     4561 tcgactcgtc gaggccgagc tccgcgagta tcccgtcgtg agcgctgcgc ttgaagtgca
     4621 acatggcgag gtagccgatg tcggcgcggc cgagaccacc cgacagctcg agggccctgt
     4681 cgatgaccgc gtagaagttt ggcatcgtcc gctcgccgag cttggccttg aacccggcct
     4741 cgtcgggtat ggagaaatgc gctcgctcga ggtcgccggg ccggggcggc caggctttgg
     4801 aaccgagggt cggcaccacg cagtcgaggc tcatcgagcc atcgccgcgg aacgacgagc
     4861 cgaggatgac gttccggcct gagttcttcc tgagcacgca ggccgagccg cccgagccga
     4921 tgtccatcat ccagcgggtc gtcggctccg agaggtcaat caggtcgttg ttccggtaac
     4981 ctgagacgag gagcacggta tccaggtcgt cccgcgcgag catcaggctc ttggccacgt
     5041 cgagggcggc catcatcgag ccgcacatag cctccatgtc gaagctccag gctccggacg
     5101 cgccgagttc gttcgccacg tacaggcccg ccagccagca tggatagtct ttatgttggg
     5161 cgccgcacca cagcacgagg ccgagatcct tgccctcgac gccggcgtcc gccagagcgg
     5221 cccgtgccgc cctgaggccc atgacggcgg tcgtatcgtc cgggccggcg accggcttac
     5281 ggcgcacgcc gaatttctcg gctacgacag cctcgggcac gccggtcgcc ttagccaagt
     5341 cggccgcgct caagaacgta tcggggacat acgtaccgat accgataaca ccgatatgca
     5401 tcatcactcc tcggtagagg tgaatcatct ctcatttact tattagtcaa ttttcacgat
     5461 acgtcaagca aattcgcagt actttatggc accagcctcg agtcttcatg gtatctagcg
     5521 aaattacttg ccacaatcgt gttgcaatcc tataattcgg cttgttactg tgaatcgtct
     5581 ctcattttgg ttcagaggag cactccaatg gttcaacgag gcgccgatac ccaacgcaag
     5641 ctcgtggaga cggcgatagc cctcttcgcg tcgcgctggt actcgtcgat atccgtggcc
     5701 gagatatgca gggccgcgga gctgtcgaac ggcgtcttct ataggtattt cccaaataag
     5761 gaagccatct ttttccatat cctcgacgat gtgatcgtgc gcatcgcggc cgccctcgag
     5821 cggaccgagg gcggcgccat agaggagcgc atcaggagca tgaccgagat actcgtcagc
     5881 ttctcggccg agcatccgga cctgatcacg gtattccgcg aaggccagta tcgttacttc
     5941 gagtacgagc gcaggctgac cgagacctac cggcgcatac tgtccaagac actcggccgc
     6001 ccggcgggag tcgccgagta cctcgtcggc ataggcgggc cgcgcttcgc cgcggtgcgg
     6061 tcggccctgc agggcgcaaa tatatcgacg gccgcgctgg cggacatggc ggcgaacggc
     6121 ctgttcccgg gcctggactg ggatcccgag cgggtcttcg ggattaccat cgcgccaccg
     6181 gcgatacgcc tcgaggagtc gagtcgcgac cgcctgatga aggcgggcaa gcggctgttc
     6241 ggggagcgcg gcttccacgc ggtcaacatc cacgaggtaa ccgacgcggc gggcctatcg
     6301 gtcggcgcgt tctacaagca tttcgacagc aaggaaagct tcttccgcga gcagatcgcc
     6361 tcggccggca aggaggtcag gcacttcatc gcgtcgaacc tgacacctgg cctgaaccgg
     6421 ctcgaggagg agatgcaagg catgttcctg ttcggcgtgt acctgtcgct cgaccgatgg
     6481 tgctacaaca tcgcccgcga aggcgaattc gtggcgttgc ccacggtcaa ggagtattac
     6541 gccgcgttca agcggggcta cgagcggctc ggggccgctg gcatggacca ggaggccgtc
     6601 gcctcggatc cgctctacct cgacagcgcc gccgagttca tgatgggcct gtcccactac
     6661 tacggcctcg aggtcgcctt cgacgaatcg ccgcacaacg cgcgggccat agtcgagggc
     6721 gtcggcgagt acctgagcca cggcctggcg cggcgatcat agcacggcat ggaggatgga
     6781 cgatggctaa agcgaagata ctcggcacgg gcgtctatgc gccgggggcg ccgatcggca
     6841 acggggagct agcgaggctc gcaggcatcg agctcgacgc agccaggctc gaggagaaga
     6901 tcggtatcag ggccaggcac atcgccaggt tgcgagggct cgacgagagc accgccgact
     6961 tcgcggagcg cgccgcccgg tccgcgatcg ccgacgcggg cctggcgccg gacgacctcg
     7021 acctcgtcat cgtcggcacc gacacgccag agtacgttag ccccgccaca gcgatcctgg
     7081 tgcagggccg actccagggc gggcagcgcg acacggcggc cttcgacgtt tccgcctcgt
     7141 gcgccagttt cgtgacggcc ctggacgccg cggccaggat gatggcgggc gacccggcgc
     7201 tacgccacgc tgtggtcgtc ggcgtgtata acatgcccgc ccacgtccgg aacggagacg
     7261 ctttcggctg gtccatcttc gccgacggcg ccggggccgt cgtcctgtcc aggacggacg
     7321 acgacgaccc gtccgggtac ataaccggca aattcatcgc tgacggcact cagtgggact
     7381 acgtcggcgt ctacgccggc ggcacccgga agcccgtgac cagggaactg ctcgacgccg
     7441 gggcctacgg cctcgagaac ctgaagcgcc tgccgggaga ccgcaacctc aagctatggc
     7501 cgccggtcat ccgtgctctg tgcgacaagg gtggcgtagg gctggaagac gtggccggct
     7561 tcgtgatgac ccagatcaac cgtagcgtca tcctggaggt catggacgag ctcggtagac
     7621 cgcgggagct cgccccgctg gcgatggatc gctacggcta tacaggctcg gcctgcgtac
     7681 cgatggcctt ccaccacgcc atcaaggacg gacggatacg ccgcggcgac ccgatagtct
     7741 tctgcgcctc gggtgccggc ctggccgtcg gcgcgaacct gtttacgtat tgagaatgta
     7801 atgacttcct gcctcttctc atcgcagtcg cttcgttcgc tttcgaaaag gaggccctat
     7861 atgtccagat gggacgatga ataccgctcg aggctacgcc cgatagacga ggcgatccaa
     7921 tgcatcgagt cgggctacga cgtcgtggtg gggcaatgcg ccagcgagcc ccagggcacg
     7981 atggcgcggc tgcatatcct ggccccccga gtccgcgacg tgcgggtgtt ctcggtgctg
     8041 acgctcaagg cctacgactt ctacctgaag ccggagatgc agggccactt cgagctggcc
     8101 agctggttcc acgccccggg ttcgcgggcc gcgctcaagg ccgggaccgg cacggtcacc
     8161 ttcgtaccga acatgctgca ccgcgcggcc acggaccgca tccacgcgag gccgcccgag
     8221 gtcttcatcg gcacctgcac cccgccggac cataacggct tcgtcagcct atcgctcggc
     8281 atcacctacg agaaagacat actcgaggcc gccaggtacg tgatactcga ggtcaacccg
     8341 cggctgccgc ggaccctcgg cgacacccac ctgcacgtca gccaggtcac gtacttcgtc
     8401 gagcacgagc aggaggtccc gagcctgcca gcgcccgagc cgagcgagac cgacctgctc
     8461 atcggccggt acatagccga cctggtcgag gacggctcga ccatccagct cggcatcggc
     8521 ggcatcccga acgccgccgc gctcgcgctc cgcggcaaga aggacctcgg cgtccatacc
     8581 gagatgctcg tcgactcgat gatggagctc tacgagatgg gcgtcatcac caaccgtcgc
     8641 aagtccctca ggaaggacaa attcgtcgcg accttcgcga tgggcagccg gaagctgtac
     8701 gactggctcg acgacaacgt ggcagtcgac ttcgagcgcg gccgctgggt caacgacccg
     8761 gcggtggtcg cccggaactc gcgcatggtg tccatcaaca cctgcctgac cgtcgacttc
     8821 accgggcagg tggccagcga gtccatcggc gcccagcagt actcaggcac cggcggccag
     8881 tcggatacgg cccagggggc ggtcgccggt ttcgacggcc tcggcaagtc gatcatcgcc
     8941 tgctactcca ccgccaaggg cggttcgctg agcaccatcg tgcccaccct gcccgagggc
     9001 agcgccgtga cgctccaccg ctccttcgtc gaccacgtcg tcaccgagca cggcgtggcc
     9061 aagcttcgcg gccgcaccgt cagggagcgc gccctcgcgc taatcgccgt cgcggccccg
     9121 gaattccgcg acgagctcaa gtcccaggcc aagcgcctcg gctacatcta ggcccgcccg
     9181 agatggcgcg atcaaggaga ccattatgtc gaaggtatcg atcgtaggag cgtataacac
     9241 caggttcggc aacctggtca agaaggacaa ggagaccggc gccgtctccg acctggccag
     9301 cctctacgag ctcatgctcg aggccggcca gggcgccctc acggacgcgg gcgtccaggc
     9361 cgacgaaata ggcggcgtct gggtcggctc gtgcgcgccc ggcctcttcg cgaaccagga
     9421 gcacgtcgcg gccttcgcac ccgagatcga cccggccggg ctacggttca agccaatgac
     9481 ccgctgcgag gacgcctgcg cttccggctc ggtggcgctt tacgacgcgc tgtacgccgt
     9541 cgaggccggt cgcgtcgggg cggccctcgt cgtcggcgtc gagaagatga acctactcga
     9601 caccgggggc gtgacccacg cgctcgcgac ctgctcgtac tggccggagg aaggcgcgag
     9661 gggcatgacc ttcccgagcc tgttcgccga gtacgccaag ggctaccgcg accgctacgg
     9721 cctggacgac gccgcgctcg cgcgcatgct cgccagcgtc gccgccctgt gctaccgcaa
     9781 cggcctcgag aacccgctgg cccatttcgg caagggcggc ccgtccgacc ggctgggcct
     9841 ggtcagcgcc gaggcgattc tcgccctgcc tccggagaag aacccgatga tagccgagcc
     9901 gctgcgcctg cacgactgct cactggtcac cgacggcgcc gcggccgtcg tcatcgtggg
     9961 cccgggccat ccggcggtca agcgaggccg cgccgtgcgc atcgccggcg tcgggcacgc
    10021 caacgagcgc atcgccatct cgaaaaggaa gaatatgtac gagctgcagg ccgccaagcg
    10081 cgcgacggct ctggccttcg ccgaagcggg catcggctcc ggagatgtgg acatagccga
    10141 ggtccacgac tgtttcacga tcaaccagct cctgtgcacc gaggccctcg gcctgtcggt
    10201 cgatggtcgc gcgggctacg actacctgga tgggcgcttc ggcccggacg acgaggcggc
    10261 ggtcaaccta tcgggcgggc tcaaggccaa gggccacccg gtcggcgcca cgggcgtctc
    10321 gatgcacgcc ctcgtctaca agcagctcgt cggcgaggcc atcggcctgg cgccgtcgaa
    10381 gacgccagaa ctaggggtga cgctgaacgt cggaggcagc gccgccacga actgcgtcac
    10441 cgtgctgcgt cgcgaacgct aggcgaacgc taggcgaacg ccatcccgtg cctagccgag
    10501 gcccagctcc gatagggcga cgcgctcatg cccgtcgcgg ccgacgaccg tctcggcgct
    10561 gtacagcgag tcgagcacgg ccgcctcgac gcagtcggtg gccgccctga agagggggtc
    10621 gagcagatcc tcgtggagcg tcgcccgcgg gagcgggccg ccccctggcg gcggatcatg
    10681 cggaacggta taggccgtgg aaaacgccag tgccagctcg cccgagcccc cgccgatcac
    10741 agcgccggtt cgtgccaggc cggaagcagc gcgcatgcac agccttcgta gctggcgcga
    10801 gtccaggggg gcgtccgtag tcaggacgac gatgaccgag ccgtcgccgg gactcccgac
    10861 gccggagccg tcgatagcgg ccgcgcgtat ccgcgcggcc gcgggaccgc cgagcgcctc
    10921 gatctccttt ccgaggagcc taccgtgaac cacgaaatcc gacaggcggc caaagttggt
    10981 caacgtgagc gtgccgacgg tatacggccg tccatcggcc agcgcgacgc ggctggcggt
    11041 gccgatgccg cccttgagtc cgtagcagct catgccgcgg ccggccccga cggagcccct
    11101 ggcgaaggct ccggccgacg ggccgccggc gccgatccgc agcgacgcct cccgggcgaa
    11161 cgagccagag cgggcgagcc gtatagcctc cgctacgtgt tccctcgtca cggccctgcg
    11221 gcgcatgtcc gacaggtagc cgtcgttgca ctcgagcgcg acgacgttgg cgctaccggt
    11281 ctcccgggcg atgtcggggt tctccgctag tagcaggtcg agggcgccgt cgacgcaggc
    11341 cgacatcgcg aaggtattcg atagcaggat cggcgactcg acgacgccga gctcctcgac
    11401 ctgcaagagc ccgaccgact tgccgaagcc gttgaagacc cggcaggcgg ccggcgcctt
    11461 gtcgcggaag aggttgcccg catggggtag caccgcggtc acgccggtct gcaggccgcc
    11521 gtcggcgatg gtcgcgtgcc caaccgagac gccggccacg tcgacgatgg agtcggtcgg
    11581 gcccgcgtcc cgggtcatga cccggagccc ccgagcgccg actcgacgat gcggtcgtcg
    11641 gcgatgtacg gcagcgttat ggacccggag ccggaataga cctcgttgta ctcggcgcag
    11701 gtcgccacgg cgtgctcgtt gcgggcccag gaacgtcggg cgaccccgcc catcacgtcc
    11761 cagggcatcg ccatcgagat gatggcgtcg actcgctcgg agccgtcgag cacgagcccg
    11821 aaaccgccgt tgacgctctt gcctatgccg actccgccgc cgttgtgcag ggcgacgagg
    11881 ctcatgccgc gcgccgcgtc gccggcgaag ttctgaacgg ccatgtcggc catcacgttg
    11941 gaaccgtcct tgatgttgct cgtctcgcgg aaggggctgt cggtgccgcc gcagtcatgg
    12001 tggtcgcggc cgagcatgac cgggccgatc tcgcccttct tgaccatgtg gttgaactcg
    12061 agggcaatct tggtacggcc gatcgcgtcc tggtacagga tacgcgcctt cgtgccgacg
    12121 acgagcctat tcttctccgc gtcccgtatc caggcccagt tatcgcggtc ctgcccgcgt
    12181 cggttcgggt cgatgcaggc catcgcggcg gcgtcggtct tgcgcaggtc ctcgtccttg
    12241 ccggacaggc agacccagcg gaacggcccg tagccgtagt cgaacagttc cggccccagg
    12301 atgtcctcga cgtagctcgg gaagacgaag ccgtccttct cgtcgacgcc gttcttggag
    12361 acctcgcgca cgccggcgtc gtagaccgcc ttgaggaacg agtttccgta gtcgaagaag
    12421 taggtaccct tgccggtcaa gcgcccgatc acgttgaagt gacggcgcag gctc
//
LOCUS       DAOPHV010000009        12452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_6014_length_12452_cov_4.203517, whole genome shotgun sequence.
ACCESSION   DAOPHV010000009 DAOPHV010000000
VERSION     DAOPHV010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 12452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12452
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6014_length_12452_cov_4.203517"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="PLQ29_00520"
     CDS             <1..1315
                     /locus_tag="PLQ29_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPG85154.1"
                     /translation="ASTLLSLAIAALLIAALHGTGAFALIRQLLSPLLAVPHAAAALG
                     LAFLLSPSGWIARALSPWATGWQQPPDLLILNDPWGLSLTFGLITKEAETVGNPAIVK
                     DLVFADEIETAKEMVKILREKEKVDVVVALVHLGMDDVYVPGSKGLAAAVSGIDLIVD
                     GHSHTLPAAPVVVNGTPIVQAGQWGMQVGKGVLTVKDGKVAAFDWEAVTINHNDKDKR
                     PVGPQFEQHPLIQPVLSDYADRVEGLLKEKIGVAAAAFPNAMTRKAETAIGDLVTDAM
                     KFITKAQGVDFAMNNGGGIRADLPAGDVTLKSIYTVLPFDNSVTVIKLKGTDVQAMFD
                     YIATTIGKGAYAQVSDGVSYTINVKAGTCENILIGGKPLDPAKVYTIATNSYLATGGD
                     GYVMFKNALSSYETSMFQRDALIAYVRQLGKDLTPVIAGRVTIVE"
     assembly_gap    1436..1535
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1923..2708)
                     /locus_tag="PLQ29_00525"
     CDS             complement(1923..2708)
                     /locus_tag="PLQ29_00525"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HPG85155.1"
                     /translation="MGRSMRAALRGFLSAFALASRIPAPLRSEPDYSALGFWMPLVGL
                     CAAAVACAGAWLGSLAFGPGPLAALASIVAQYLAFNLFHLDGLLDTADAAGVNGDAER
                     RRTVLKDPRVGSFALFAGFAALAARLGAVTALAERGGPVFWGALCLAPVAGRFGSMLV
                     TAFCEPYPGGGLAAMIGRPSPVSSTVGYAVAAAPAALLFGAAYGPIGAAAAMLAGGLA
                     AVATAAFVGYWYGKRLDGYSGDALGAAVELGELAVLLLAVAVA"
     gene            complement(2693..3394)
                     /gene="cbiR"
                     /locus_tag="PLQ29_00530"
     CDS             complement(2693..3394)
                     /gene="cbiR"
                     /locus_tag="PLQ29_00530"
                     /inference="COORDINATES: protein motif:HMM:NF041277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobamide remodeling phosphodiesterase CbiR"
                     /protein_id="HPG85156.1"
                     /translation="MSSRVSVPSYVIPGSYVENLRFLYERTSQRQVELLFFIYDDDAR
                     AMLRSEAREIESYSADFGYTVHMPDAIEPGHEELLERTADYASAFIVHPPRADDGLRA
                     FVALLDEWRGRYGPDRFLIENTTLARFDAADTAAADSNYGPPRVCADVGHLRAEGRDP
                     AAWIAERADRVGELHVHGFDGSRDHVGFAADEPWILELAPFARGFDGIVELELFSWAE
                     AEAGSAVLRDAWGAL"
     gene            complement(3391..3873)
                     /locus_tag="PLQ29_00535"
     CDS             complement(3391..3873)
                     /locus_tag="PLQ29_00535"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HPG85157.1"
                     /translation="MRVLLTGGVKSGKSSRALELARDFAPDRWFLATATPFDDEMRER
                     IRRHRAERATDFVTVEEPIDIDAAVRERMVVDCVPMWLNNLFFADREDDWEPILDRFI
                     ARLPRDIVIVTNEIGMGVIPADPTSRRYGMALGRANAKLAAAVDSVELLVAGIPMKVK
                     "
     gene            complement(3918..4601)
                     /locus_tag="PLQ29_00540"
     CDS             complement(3918..4601)
                     /locus_tag="PLQ29_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPG85158.1"
                     /translation="MSKEETIFGDDWPYSKAKSSLLRSAAIVIREQGPRSATLKNISG
                     RAGVTEPAIFRHFDGVDGMFESLHAVAALFYARFTACLEGDEPTGMDRLEAGVSRIFD
                     SLAANADFAYVVAKPDPIFRQYPRLKEKDAGMYASLAEAFTDCFKSAKADGSLLPGAD
                     PSALASAALGLFYQVLYAWVDDVEGYSLHRDGMKAFRAYLAIARNPGRAASSGAKAPA
                     KTKGAAKKR"
     gene            complement(4793..5503)
                     /locus_tag="PLQ29_00545"
     CDS             complement(4793..5503)
                     /locus_tag="PLQ29_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG85159.1"
                     /translation="MLRIDDLSVHYGGIHALQGISFEVPEGKIVTLIGANGAGKSTTL
                     KSVVGLVKPSGGSISLDGERVSGLATRDIVRRGVVLAPEGRRIFPDLSTEENLELGAF
                     MRDDKVGVAADKKRMYALFPRLEERRKQKAGTLSGGEQQMLAVARALMSRPKLLMMDE
                     PSLGLAPLIVKMIFDIVKAINAEGTTILLVEQNAKAALEIADFGYVLETGRITMSGPG
                     RELLADDGVRRAYLGESL"
     gene            complement(5503..6234)
                     /locus_tag="PLQ29_00550"
     CDS             complement(5503..6234)
                     /locus_tag="PLQ29_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG85160.1"
                     /translation="MRFGGLTAVSDLNIEIQKNEIVGLIGPNGAGKTTAFNAITGVYR
                     PTEGRVVFKGRDITGQKPHAITKLGIARTFQNIRLWTDMDVLENVLVACHLRMDVGVF
                     ASTLHLPGYRVKERRSREFAMSLLESVGLADQAREAATSLPYGKQRRLEIVRALATEP
                     SLLLLDEPAAGMNPQESLELMDFIGSIRDRYDLTILLIEHHMQVVMGVCTRMYVLDYG
                     VTIAHGTPDEIRRDPKVIEAYLGVD"
     gene            complement(6255..7313)
                     /locus_tag="PLQ29_00555"
     CDS             complement(6255..7313)
                     /locus_tag="PLQ29_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPG85161.1"
                     /translation="MKIKRNTLLTLAGLALLVGAIAAADATLGRFSVRILNLSAIYIV
                     LALSLNLLNGFTGLFSLGHAGFMAVGAYVSALLTMSPAQKDMNFFLEPIMPFLRDIQL
                     PFPLALLAAGACAGLVAVVIGVPALRLRDDYLAIATLGFAEIIRVLITNAQSLTNGAQ
                     GLKAITKYSTTWVVWSVAALSVVFMLALVRSSYGRTMKAVRDDEVAAEAMGVNVFKVK
                     STSFVISSFMAGVGGALLGHMITTIDPKMFTLTLTFNILLIVVLGGNGSITGSVIGAI
                     VVTVLMEALRFLDMPMNFLFIKTNGLPGLRMVIFSILLMIVVLYRQRGLMGNKEFDWD
                     GFIGIFRRKGASSAGGRS"
     gene            complement(7318..8211)
                     /locus_tag="PLQ29_00560"
     CDS             complement(7318..8211)
                     /locus_tag="PLQ29_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPG85162.1"
                     /translation="MELSTFFQQLTNALSLGSLYALIAIGYTMVYGILRLINFAHGDV
                     FMLGAYFAFYAATSLAMPWWVAFIVAIGLTAIFGMGLERVAYRPLRDSPKISVMISAI
                     GASFLIENVGIVVFGGRPKSFPSIPLFDTVLHLGPVSVLSVSLFIPVITAAILVALLW
                     IVNGTKTGLAMRAVSTDLDAARLMAIDVNRTISLTFGIGSLLAGLGGIMWTMKYPQLN
                     PLMGVMPGLKCFIAAVIGGIGNIPGAVLGGFLLGVLEIMIVAFLPELTGYRDAFAFVL
                     LIVVLLVKPSGLLGKHQAVKV"
     gene            complement(8349..9506)
                     /locus_tag="PLQ29_00565"
     CDS             complement(8349..9506)
                     /locus_tag="PLQ29_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPG85163.1"
                     /translation="MKKAILVIVAIMLVASIVGCSKAETKTDANVVKFGVFEPLTGAN
                     AAGGAEELDGVRLAQELYPTVTVGGKEYKIELAIADNKSDKVEAANAGQRLVDSDKVQ
                     VVLGSWGSGLSMAAGPVFKDAKIPAIGLSCTNPLVTKDNPFYFRVCFLDPFQGTVMAN
                     YAFKDVKARKAVIIREVSNDYSVGLAKFFADSFKSLTGDQGAILAELNYNTNDQDFSA
                     QLTQVKSLKPDVIFAPGNYTESALIIKQARELGITAPFLGGDTWEIAEFINVGKQAVE
                     GAGMSAFFDNDAPLTPLSKVFVESYQKKYGKLPSGTAVLGFDGYLLAIDAIKRADSVD
                     PLKIRDAIAATKDFAGAAGYVTLDANGDPIKSAVIKEVKGGKFVFKTTVAP"
     gene            9738..10565
                     /locus_tag="PLQ29_00570"
     CDS             9738..10565
                     /locus_tag="PLQ29_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPG85164.1"
                     /translation="MSESAIKAFDLLFHLSRAGSSSLARLSRETGWDKATALRYLTAM
                     EKRGVVERRDGDWTLGLALLELASRVPITENIANRVKPVLNDLAKATGETANLSALTG
                     SELMYLAKADGGRALRLRSNPGDRMPLYCTAAGKCILSRFSVPLRAQYLAETHLSAIA
                     PKTIVDPEKLAEDIERTIRRGWAIDDEELESGLICYAMPLDLPRYGFSGAISISGPSG
                     RMLQAQEEILSSLRWAVMTLQEDLGGGIGDEGPQLFSPARGGFKSTDWHRPGSETYE"
     gene            10562..10744
                     /locus_tag="PLQ29_00575"
     CDS             10562..10744
                     /locus_tag="PLQ29_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPG85165.1"
                     /translation="MTPARIERARCVTCRRCVAACPEGAISVDGDACVVDRGRCVACG
                     RCAAACRTDAVRLVWA"
     gene            complement(10781..11182)
                     /locus_tag="PLQ29_00580"
     CDS             complement(10781..11182)
                     /locus_tag="PLQ29_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPG85166.1"
                     /translation="MNNLNSIIIEGNLTRDPQLKTIPNGNQVCSFAVASNRYYRHNDQ
                     TEQEVSFFEVETWSKLAQTCGEVLKKGRGVRVVGRLKQDRWEDPNGKTQARIKIVAEH
                     VEFKPVFKRDDEAGAEKAAMVAEDTEAETVF"
     gene            11370..12152
                     /locus_tag="PLQ29_00585"
     CDS             11370..12152
                     /locus_tag="PLQ29_00585"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPG85167.1"
                     /translation="MITYGEGFGPITPRSLKDEFIERFEALILSGKFAPGDKVPSERD
                     LGVLFGISRPVVHEGLRALESRGLVTIESRKGARVNDYRREGSIEMLLSILNYTGGKL
                     SQSLLDGILELRLLFEVETSRLAALRRSASHVDELRSIVARERSMVESDADGRAASSR
                     VASPRAASAREVADIDLELHISIAIASGNEIYPLLMNSFRRIYLAILEEFYADASVVG
                     PVFEYHERLVEAIAASDEDGARGVMLEMLGYSERNLRRILFG"
     gene            complement(12141..>12452)
                     /locus_tag="PLQ29_00590"
     CDS             complement(12141..>12452)
                     /locus_tag="PLQ29_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85168.1"
                     /translation="ARFDGAVLKGENPFWTLTVLPPYALLQIASEASPAVIRYTSISD
                     GEAGLVIDGAGEVGGWSHRYRITILERPAVAPDGATYPLTVTIDWSDGTRYSGWGSLA
                     E"
BASE COUNT         2146 a         4039 c         4023 g         2144 t
ORIGIN      
        1 tgcctccacc ctgctgtcgc tggccatcgc cgccctcctg atcgcggcgc tgcatggtac
       61 cggtgccttc gcgctgatcc ggcagctgct gtcgccgctt ctggcggtgc cccatgccgc
      121 cgccgcgctg ggcctggcct ttctgctgtc gccctcgggc tggatcgcgc gggcgctgtc
      181 gccctgggcg accggctggc aacagccgcc cgatctgctg atcctgaatg atccatgggg
      241 cctcagcctg accttcggcc tgatcaccaa ggaagccgag accgtcggca acccggccat
      301 cgtcaaggac ctcgtgttcg ccgacgagat cgagaccgcc aaggaaatgg tcaagatcct
      361 ccgcgagaag gagaaggtcg acgtcgtggt cgccctcgtc cacctcggca tggacgacgt
      421 ctacgtgccc ggctccaagg gcctcgccgc ggccgtctca ggcatcgacc tgatcgtcga
      481 tggccacagc cacaccctgc ccgcggcccc ggtcgtggtc aacggcaccc cgatcgtcca
      541 ggccggccag tggggcatgc aggtcggcaa gggcgtcctg accgtcaagg acggcaaggt
      601 cgccgccttc gactgggaag ccgtcaccat caaccataac gacaaggaca agcggccggt
      661 cggcccgcag ttcgagcagc acccgctcat ccagcccgtg ctgtccgact acgccgacag
      721 ggtagagggc ctgctcaagg agaagatcgg cgtagccgcc gctgccttcc cgaacgcgat
      781 gacccgcaag gccgagaccg ccatcggcga cctcgtcacc gacgcgatga agttcatcac
      841 caaggcccag ggcgtcgact tcgccatgaa caacggcggc ggcatccgcg ccgacctgcc
      901 cgccggcgac gtcaccctca agtcgatcta cacggtgctg cccttcgaca actccgtcac
      961 ggtcatcaag ctcaagggca ccgacgtgca ggccatgttc gactacatcg ccaccaccat
     1021 cggcaagggc gcctacgccc aggtctccga cggcgtcagc tacacgatca acgtgaaggc
     1081 cggcacgtgc gagaacatcc tcatcggcgg caagcccctc gaccccgcca aggtctacac
     1141 catcgccacc aactcctacc tggcgaccgg cggcgacggc tacgtcatgt tcaagaacgc
     1201 gctgtcctcc tacgagacca gcatgttcca gcgcgacgcc ctcatcgcct acgtccgcca
     1261 gctcggcaag gacctgaccc cggtcatcgc cggccgcgtc accatcgtcg agtaattcca
     1321 ggtcgacgat agacttcgta tgataaggcc gcccctcggg gcggccttct tttcgtccat
     1381 ggcgcggcgt cggcttcgtc gctagcccgg ggcgcgcctc gacatgcacg gagcannnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngctcc tcgccgtcat ccacgccatg
     1561 gacgaaaagc ccggttcgct cggcgcggcg tcggcttcgt cgctagcccg gggcgcgcct
     1621 cgacatgcac ggagcatgcc gtcggcgctc cccgggcgtc gctcctcgcc gtcatccacg
     1681 ccatggacga aaagcccggt tcgctcggcg cggcgccggc ttcgccgcta gcccggggct
     1741 cttatggtgt catgaagctt gtaggtgatg cacgtgtacc catttctatt gcaccacggc
     1801 catcagtatg gagacccggc gtctgcgtac gggctatgag atagtcgtca tccgcctata
     1861 gaatgaaacc gctaagattc gtgacactaa gatcggcctg ctcgttaggc gcagttcggt
     1921 agctaggcga cggcgacggc tagtagcaat acggccagct cgccgagctc gacggcggct
     1981 ccgagcgcgt cgcccgagta gccgtcgagg cgcttgccgt accaatatcc gacgaaggcc
     2041 gccgtggcca cggccgctag gcctccggcc agcatggcgg cggccgcgcc gatcggtccg
     2101 taggccgcgc cgaacagtag cgcggccggc gcagccgcca ccgcgtagcc gacagtcgat
     2161 gacaccggag acgggcgccc gatcatggcg gcgaggccgc cgcccgggta aggctcgcag
     2221 aaggccgtta cgagcatcga accgaagcgt ccggcgaccg gcgctaggca gagggcgccc
     2281 caaaacaccg ggccgccgcg ctcggccagc gccgtgacgg cgccgagcct cgccgccagg
     2341 gcggcgaaac cggcgaacag ggcgaacgag ccgacgcgag gatccttgag taccgtgcgc
     2401 ctccgctccg cgtcgccgtt cacgccggcg gcgtcggccg tatcgaggag cccatccagg
     2461 tggaacaggt tgaaggccag gtattgggcg acgatcgagg cgagcgccgc caggggcccc
     2521 gggccgaagg ccagcgagcc gagccaggcg ccggcgcagg cgacggccgc cgcgcatagc
     2581 ccgaccaggg gcatccagaa tccaagtgcg ctataatccg gttcggagcg gagcggcgcc
     2641 ggtatcctgg aggctagggc gaacgcgctc aggaagccgc gcagggccgc cctcatagag
     2701 cgccccacgc gtcgcggagt acggccgatc ccgcctcggc ctcggcccac gagaatagct
     2761 ccagctcgac aatgccgtcg aagccgcgcg cgaacggggc tagctcgagt atccacggct
     2821 cgtcggcggc gaagccgacg tggtcgcgcg agccgtcgaa gccgtggaca tggagctcgc
     2881 cgacgcgatc ggcccgctcc gcgatccagg cggccgggtc gcgcccctcg gcgcgtaggt
     2941 gccccacgtc ggcgcagacg cgcggagggc cgtagttcga gtcggcggcg gcggtgtcag
     3001 ccgcgtcgaa cctggccagc gtcgtgttct cgatcaggaa gcgatccggt ccgtagcgtc
     3061 cccgccactc gtcgagcagg gcgacgaagg cccggaggcc gtcgtcggcg cgcggggggt
     3121 gcacgatgaa cgccgacgcg tagtccgccg tgcgttcgag cagctcctcg tgcccgggct
     3181 cgatggcgtc gggcatatgc accgtgtagc cgaagtctgc cgagtacgac tcgatctccc
     3241 gggcctcgct acgtagcatg gcgcgcgcat cgtcgtcgta gatgaagaac agcagctcta
     3301 cctgccgctg gctcgtgcgc tcatagagga aacgtaggtt ctcgacgtag ctcccgggga
     3361 tcacgtagga cggcacggag acgcgggagc tcatttgacc ttcatcggga tccccgcgac
     3421 gaggagctcc accgagtcga cggcggcggc cagcttggcg ttggcgcgcc cgagcgccat
     3481 accgtagcgg cggctcgtcg ggtcggcggg gatcacgccc atgcctatct cgttggtgac
     3541 gatgacgata tcgcgcggca ggcgcgcgat gaaccggtcc aggatgggct cccagtcgtc
     3601 ctccctgtcg gcgaagaaga ggttgttgag ccacatcggt acgcagtcga cgaccatgcg
     3661 ctcgcgcacc gcggcgtcta tgtctatagg ctcctcgacg gtgacgaaat ccgtggcccg
     3721 ctccgccctg tgcctgcgga tgcgttcacg catctcgtcg tcgaagggcg tagccgtcgc
     3781 caggaaccat cggtccggcg cgaagtccct ggctagctcg agcgctcggc tcgacttgcc
     3841 cgacttcacg ccgccggtca gtagtactcg catgactcga cagtaacggc cgctcccggg
     3901 cgcgtcaagc cgacgggtca gcgcttcttg gcagcgccct tggtcttggc tggagccttg
     3961 gcgccggacg aggcggcccg gccagggttc ctggcgatag ccaggtaggc ccgaaaggct
     4021 ttcatgccgt ctctatggag gctgtagccc tcgacgtcgt cgacccaggc gtagagtacc
     4081 tggtagaaga ggcccagggc cgccgaggcc agcgccgagg ggtccgctcc gggcaggagg
     4141 ctgccatcgg ccttggcgga cttgaagcag tcggtgaaag cttcagccag ggaagcgtac
     4201 atcccggcgt ccttctcctt gagcctcggg tactgcctga agatcgggtc gggcttggcg
     4261 acgacgtacg cgaagtcggc gttggcggcc agcgagtcga atatacggct gacgccggcc
     4321 tcgagcctgt ccatgcccgt cggctcgtcg ccctcgaggc aggccgtgaa tcgcgcgtag
     4381 aatagcgccg ccacggcgtg caacgactcg aacatgccgt cgacgccgtc gaagtgcctg
     4441 aatatggcgg gttcggtcac cccggctcta ccggagatat tcttgagggt agccgaacgg
     4501 gggccctgtt cacgtataac gatggccgcc gaacggagta gggacgactt agccttcgaa
     4561 tacggccaat cgtcgccgaa aatggtctct tccttgctca tgccgatatg ctatagacat
     4621 ccgcggcgaa tgagaagcga gatcgccaca gtacaggtac aatttactgg ttctttacga
     4681 atatcatatt cctatagcaa caaaaatgaa cgctccgcac ggttcgtccg caaggccggg
     4741 cgacgccggc gtggcgcggg ccggtcccca aggcggccgc cccgctccgc gcctagaggc
     4801 tctccccgag ataggcccta cggaccccgt cgtcggcgag cagctcgcgg cccggccccg
     4861 acatcgtgat gcggccggtc tcgagcacgt agccgaagtc ggctatctcg agggccgcct
     4921 tggcgttctg ctcgacgagc aggatcgtgg tgccctcggc gttgatggcc ttgacgatgt
     4981 cgaagatcat cttgacgatg agcggggcca atccgagcga cggctcgtcc atcatcagga
     5041 gcttggggcg ggacatcagc gcccgcgcca cggcgagcat ctgctgctcg ccgccggaca
     5101 gggtgccggc cttctgcttg cggcgctctt cgagccgcgg gaacagcgcg tacatgcgtt
     5161 tcttatccgc ggccacgccg accttatcgt cgcgcatgaa ggcgccgagc tccaggttct
     5221 cctcggtgct caggtccggg aagatcctgc gtccctcggg cgcgagtacc acgccgcggc
     5281 gcacgatgtc cctggtcgcc agcccggata cgcgctcccc gtccaggctg atcgatccgc
     5341 cgctcggctt gaccaggccg acgacggact tgagcgtcgt gctcttgccc gcgccgttcg
     5401 cgccgatcag cgtgacgatc ttgccctccg gcacttcgaa cgagatgccc tgcagggcgt
     5461 ggatgccgcc gtaatgtacc gacaggtcgt ctatcctgag catcagtcca cccccagata
     5521 ggcttcgatg accttgggat cgcggcggat ctcgtcggga gtcccgtggg ctatcgtcac
     5581 gccgtagtcg aggacgtaca tgcgcgtgca gacgcccatc accacctgca tatggtgctc
     5641 gatcaacagt atggtcaggt cgtagcggtc cctgatggag ccgatgaagt ccatgagttc
     5701 gagcgactcc tgcgggttca tgcccgctgc cggctcgtcg agcaggagga gcgaaggctc
     5761 ggtggccagc gcccgcacta tttcaagccg gcgctgcttc ccgtatggta gcgacgtggc
     5821 cgcctcgcgc gcctggtcgg ccaatccgac cgactcgagc agcgacatgg cgaactcgcg
     5881 cgagcgtcgt tccttgacgc ggtagccggg cagatgcagc gtcgaggcga acaccccgac
     5941 gtccatccta aggtggcagg cgacgagcac gttctcgagc acgtccatgt ccgtccagag
     6001 ccgtatgttc tggaaggtcc tcgcgatgcc gagcttggtg atcgcgtggg gcttctggcc
     6061 ggtgatgtcg cggcccttga agaccacccg cccctcggtc ggcctgtaga cgccggtgat
     6121 ggcgttgaag gcggtggtct tgcccgcgcc gttcgggccg atgaggccga caatctcgtt
     6181 cttctggatc tcgatgttca ggtcggacac ggcggtcagg ccgccaaagc gcatcgagac
     6241 gtccctggtg cttatcacga gcggcctccc gcgctcgacg cgcccttccg ccggaagatc
     6301 ccgatgaagc cgtcccaatc gaattccttg ttacccatga gcccccgctg ccggtatagc
     6361 acgacgatca tcagcagtat cgagaagatg accatgcgga gccccggtag cccgttcgtc
     6421 ttgatgaaca ggaaattcat gggcatgtcc aggaatcgga gcgcctccat cagcacggtg
     6481 acgacgatgg ctcctatcac cgagccggtg atcgagccgt taccgccgag cacgacgatc
     6541 agcaggatgt tgaaggtgag cgtcagcgtg aacatcttgg ggtcgatggt tgttatcatg
     6601 tgcccgagca gggcgccgcc gacgccggcc atgaagctgg agatgacgaa gctcgtcgac
     6661 ttgaccttga acacgttgac gcccatggcc tcggcggcca cctcgtcgtc ccgcaccgcc
     6721 ttcatcgtgc ggccgtagct cgagcgcacg agggccagca tgaacacgac cgatagggcc
     6781 gctaccgacc agaccaccca ggtcgtcgag tacttggtga tggccttgag gccctgggca
     6841 ccgttcgtca ggctctgcgc gttcgtgatg agcacacgga tgatctcggc gaagccgagc
     6901 gtcgcgatgg ccaggtagtc gtccctgagc cggagcgccg gtacgccgat gacgacggcg
     6961 accaggccgg cgcaggcccc ggcggccagg agggccaaag ggaacggcaa ctggatgtcg
     7021 cgcaggaagg gcatgatggg ctccaggaag aaattcatgt ccttctgcgc cgggctcatc
     7081 gtcaggagcg cgcttacgta cgcgccgacc gccatgaagc cggcgtggcc gagcgagaag
     7141 aggcccgtga acccgttgag caggttgagc gacagggcta gcacgatata gatggccgag
     7201 aggttgagta tcctgaccga gaatctgccg agcgtcgcgt cggccgcggc aatggctccg
     7261 accaaaaggg cgaggcccgc gagggttagt agcgtgttcc gcttgatctt catagtgtca
     7321 caccttgacc gcttgatgct tgccgagcag gcccgacggc ttgacgagga gtacgacgat
     7381 cagtagcacg aacgcgaacg cgtcgcggta gccggtcagc tccggcagga acgccacgat
     7441 catgatctcg agcacgccga gcaggaagcc gccgagcacg gcgccgggaa tgttgcctat
     7501 gccgccgatc acggcggcga tgaagcactt gagcccgggc atcactccca tcagcgggtt
     7561 cagctggggg tacttcatcg tccacatgat gccgccgagg ccggccagca gcgagccgat
     7621 gccgaaggtc agcgagatcg tcctgttcac gtcgatggcc atcagcctgg ccgcgtccag
     7681 gtcggtggag acggcgcgca tggccagccc ggtcttcgta ccgttgacta tccagagtag
     7741 cgcgacgagt atggcagccg tgatgaccgg gatgaacagg ctgaccgaca gcaccgacac
     7801 cgggccgagg tgcaacaccg tgtcgaacag cgggatgctc gggaagctct tggggcggcc
     7861 gccgaacacg acgatgccga cgttctcgat caggaaggac gcgccgatcg cgctgatcat
     7921 caccgagatc ttcggcgagt cacggagcgg ccggtacgcg acccgctcca ggcccatgcc
     7981 gaatatcgcc gtcaggccga tcgccacgat gaaggctacc caccacggca tcgcgagcga
     8041 ggtcgcggcg tagaacgcga agtacgcgcc gagcatgaag acgtcgccat gggcgaagtt
     8101 tatcaggcgt aggataccgt agaccatcgt gtagcctatc gctatgaggg cgtagaggct
     8161 accgaggctc aacgcgttgg tcagttgctg gaagaaagtc gatagttcca ttggttcggg
     8221 tcctcgggcg ccgcgatcgc ggctgtaaac gaggacgggc gcctccgtcg gcgtggacgg
     8281 ctgcgcccgt cgcgcgtccg gccgtcgcga ggcggcggac cggctacgct atctatggat
     8341 ggcggccgtc aaggggctac ggtcgtcttg aacacgaact tgccgccctt cacttccttg
     8401 atgacggcac tcttaatcgg gtcgccgttg gcgtcgaggg tcacgtagcc agcggcgccg
     8461 gcgaagtcct tggtggccgc gatggcgtcg cggatcttga gcgggtcgac gctgtcggcg
     8521 cgcttgatcg cgtcgatcgc gagcaggtag ccgtcgaagc cgagcaccgc ggtgcccgag
     8581 ggcaacttgc cgtacttctt ttgatacgac tcgacgaaga ccttggatag cggggtcagc
     8641 ggggcgtcgt tgtcgaagaa ggccgacatg ccggcgccct cgacggcctg cttgccgacg
     8701 ttgatgaatt cggcgatttc ccaggtgtcg ccaccgagga agggcgccgt gatgcctagc
     8761 tcgcgggcct gcttgatgat cagggcgctc tcggtgtagt tgccgggggc gaagatcacg
     8821 tccggcttga ggctcttgac ctgcgtgagc tgggccgaga agtcctggtc gttggtgttg
     8881 tagttgagtt cggcgagtat cgcgccctgg tcgccggtaa gggacttgaa gctgtcggcg
     8941 aagaacttgg ccaggccgac cgaatagtcg ttcgagacct cgcggatgat gaccgccttg
     9001 cgcgccttga cgtccttgaa ggcgtagttg gccatgacgg tgccctggaa cgggtcgagg
     9061 aagcagacgc ggaagtagaa ggggttgtcc ttcgtgacca gggggttggt gcacgagagg
     9121 ccgatggccg ggatcttagc gtctttgaag accggaccgg ccgccatcga gaggcccgag
     9181 ccccatgagc cgagcacgac ctgaacctta tcgctgtcga ccaggcgctg accggcgttc
     9241 gcggcctcga ccttgtcgga cttattgtcg gctatcgcca gctcgatctt atattccttg
     9301 ccgcctacgg tgaccgtcgg gtacagctcc tgagccaacc tgacgccgtc gagctcctcg
     9361 gctccgccgg ccgcgttcgc gcccgtgagg ggctcgaaga cgccgaactt cacgacgttc
     9421 gcgtccgtct tcgtctccgc cttggagcag ccgacgatgg acgcgaccag catgatcgcg
     9481 acgatgacca gaatcgcttt cttcatacgt attcctcctt ggacggatgc gcggcgggcc
     9541 cttgacggct ctgagcgtgc atacatcgta ggaacagata tatccgttgt caaatatttt
     9601 atcaagagaa ttctacaaaa agaaacaaaa tggctagttg tatggtttct attgacaata
     9661 gtgcgaaaat gcaagtacgt tgtaagttgc cgtttcattt tatacgcaat ctattttggc
     9721 catagaaacg tggagatatg agcgaatcag cgatcaaagc tttcgatcta ttgtttcatc
     9781 tatcacgagc tgggagctcg tcattggccc gcctgtcccg agagaccggc tgggataagg
     9841 cgaccgcgct gcgatacttg accgcgatgg agaagcgtgg cgtcgtcgag cgcagggatg
     9901 gcgactggac cctcggcctg gccctactcg agttggccag ccgcgtgcct atcaccgaga
     9961 atatcgccaa ccgggtcaag ccggtgctga acgacctggc caaggccacc ggcgagacgg
    10021 ccaatctgtc ggcgctgacc ggctccgagc tgatgtacct agccaaggcc gacggcggcc
    10081 gcgccctccg cctccgatcc aacccgggcg accgtatgcc cctctattgt acggccgccg
    10141 gaaagtgtat cctgtcccgc ttctcggtcc cgctacgcgc tcagtacctg gccgaaacgc
    10201 acctgtccgc catcgcgccc aagaccatcg tcgatccgga gaaactagcc gaggacatcg
    10261 agcggaccat ccgccgcggc tgggcgatag acgacgagga gctcgagtcc ggactcatct
    10321 gctacgccat gccgctcgac ctaccgcgct atggcttctc cggggcgatc agcatttccg
    10381 gcccgtcggg caggatgctc caggcccagg aggagatcct gtcttcgctc cgctgggcgg
    10441 tcatgacact ccaggaagac ctaggcgggg gcataggcga cgaaggtccg cagctgttca
    10501 gccccgcccg aggcggcttc aagagcaccg actggcacag acccggctcg gaaacctacg
    10561 agtgaccccc gcccgcatcg agcgggccag gtgcgtaacc tgccgccgtt gcgtcgcggc
    10621 ctgtcccgag ggcgccatat cggtcgacgg cgacgcctgc gtcgtcgacc gggggcgttg
    10681 cgtcgcttgc ggccgctgcg ccgctgcctg caggaccgac gccgtccgac tcgtctgggc
    10741 ctgagccggc gaccgagacg ggacggtcgc ttcccgccgc ttagaatacc gtctcggcct
    10801 ccgtgtcctc tgctaccatc gccgccttct ccgctccggc ttcgtcgtcg cgcttgaaaa
    10861 cgggcttgaa ctcgacgtgc tcggcgacga tcttgattcg agcctgcgtc ttgccgttcg
    10921 ggtcctccca gcggtcctgc ttgaggcgcc ctacgacccg gactccgcgg cccttcttga
    10981 gtacctcgcc gcaggtctgg gcgagcttgg accaggtctc gacctcgaag aacgacacct
    11041 cctgctcggt ctggtcgtta tgccggtagt agcggttcga cgcgaccgcg aacgagcaaa
    11101 cctggttgcc gttcgggatc gtcttgagct gggggtccct cgtcaggttg ccttcgatga
    11161 tgatggagtt caggttgttc aacatgcttc ctccctcgac cgaccctagg tcgatcgcca
    11221 acgctttgat cggcgcaacc cgcgcctcgg gccgacgcgc tcggcgtcga cggctcggat
    11281 acgccgcttc aagccacggc gcttgcgcgc gtcggccgtt ttgccatagg attggccggt
    11341 tcctggctat accagattaa gagggtgcga tgatcacata cggggaaggc ttcggtccga
    11401 ttacgcctcg atcgctcaag gacgaattca tcgagcgctt cgaggcgttg atactttccg
    11461 gcaaattcgc cccgggcgat aaggtccctt cggagcgcga cctgggcgta ttgttcggca
    11521 tatcgaggcc tgtcgtccac gaggggctac gcgccctcga gtccagggga ctcgttacga
    11581 tcgagagccg caagggagcg cgcgtcaacg actatcgtcg cgaaggctcg atagagatgc
    11641 tgctgtcgat actgaactac accggcggca agctgtcgca gtcgctgctc gacggcatcc
    11701 tggagctgcg cctcctgttc gaggtggaga cctcccggct cgcggctctg cgacggtccg
    11761 cgtctcacgt cgacgagcta cggtccatcg tcgcccgcga gcgctcgatg gtcgaatcgg
    11821 acgcggacgg cagggccgct tcatcgcgcg tggcgtcacc tcgcgctgcg tcggcccgcg
    11881 aggtggccga tatagacctc gaactgcata tatcgatagc gatagccagc ggcaacgaga
    11941 tctatccact cctgatgaac agcttcaggc gcatctacct cgcgatactc gaggagttct
    12001 acgccgacgc ctcggtcgtc gggccggtgt tcgaatacca cgagcgcctc gtcgaggcga
    12061 tcgccgcgag cgacgaggac ggcgcccgcg gggtgatgct cgagatgctc ggctacagcg
    12121 agcgcaacct gcggcgcatc ctattcggct aggcttcccc agccggagta acgagtgccg
    12181 tcggaccagt ctatcgtcac ggtcagcgga taggtcgctc cgtccggtgc gacggctggg
    12241 cgttccagta tcgtgatcct atagcggtgg ctccagccgc cgacctcgcc ggcgccgtct
    12301 atgacgaggc ccgcctctcc gtcggagatc gacgtgtagc ggatgacggc cggactcgct
    12361 tccgacgcta tctgtagcag cgcgtagggc ggtagcacgg tcagggtcca gaaggggttc
    12421 tcgcccttga gcaccgcgcc atcgaagcgg gc
//
LOCUS       DAOPHV010000010        12297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_6129_length_12297_cov_4.027283, whole genome shotgun sequence.
ACCESSION   DAOPHV010000010 DAOPHV010000000
VERSION     DAOPHV010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 12297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12297
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6129_length_12297_cov_4.027283"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PLQ29_00595"
     CDS             complement(<1..715)
                     /locus_tag="PLQ29_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85169.1"
                     /translation="MGSQERKAVIELPVKVVLTDSGTTYFIKNNKKLRKFKLADNVEE
                     YGILLDHFTPASLQRMMLIDYVSKVEISDSEFVKIRQEVMDISKLVTYSMMYRQYDSY
                     IFQRVLSSDVIKNWNRKNPANIIDEKTKINDVFLQAFIKDKDKEIAEIKRTVLAPMYA
                     LINRNSNLLPEEKNIQLLLSEKFLNTLRPFTWFIIAKFHDSDGYDTLIKDIRTSLAEY
                     MEKAKIAEYVALNVMELAAN"
     gene            827..2410
                     /locus_tag="PLQ29_00600"
     CDS             827..2410
                     /locus_tag="PLQ29_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85170.1"
                     /translation="MFRHLSIAVLAIIASGSLASLTLGAQEASNDPRTVEISGAFDLV
                     SARAIGTGGATVAFVAAAGGEGGAASEAALDAALHALRSASVHGYTAYIMLFDPEPAA
                     IPRASEPGDPLIREPSLARALADMGMDAVVLLDLGPSGGAMGNGSMQGPAVRAASLGM
                     MTPRSVLEAVRVASRAAGLRIRESPVEDFYASAGLSPGSKALAPWLEAGLAAVAIESR
                     PEDIETFAALAPAIAGAVAGQVAALVSEDSAVPGSDDVGYLRYPAPTGPVLVSDAAIT
                     LGTITTTALVAAVLALGLLNGRRRKASLEAVAKEAAGAFALSFIALFGARALSLLAAS
                     AVRALGDPTGPGSPSWAVAVALVLRVAGTLSLYYTASGLASRLGLHGDHRRIDAARAS
                     LGLLCLDALVAVVVFPPVAPFLLAAVIVSALASERAVGAAMGLVAVSLLALPFFDPRV
                     IAAIGSARGAESVAQAMLSAGLRGNAVAAAFASPFGLWLNAASSPETRLRRGARIAPL
                     WLVAAIVCAVGEAVLTASL"
     gene            2421..3605
                     /gene="dinB"
                     /locus_tag="PLQ29_00605"
     CDS             2421..3605
                     /gene="dinB"
                     /locus_tag="PLQ29_00605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPG85171.1"
                     /translation="MTSVFFHIDLDAFFAAAEVLDDPSLAGRPVIVGAAPGHRGVVST
                     CSYEARRYGVHSAMPISEAYRLCPEAAFRPVRMERYAELSARVMAVFEAYTPDVVRVS
                     IDEASLDMTGTERLWGAPREAAEAIRDRVARETGLSISIGIAPNRYVAKVASGVRKPA
                     GLVVVEPGDEAAFMAGLRLKDLWGVGPKTRARLEELGIGDQARLLELSRATLESNFGK
                     AGGEFIYGVARGVDPGIYAGEARSRSISSETTYERDEAGLPRIETTLLGMAEELVARL
                     YADGYSSRCVALKLRYGDFETVGVRETLDEPFFDSGAVYEAARSLLARKWDGRPLRLV
                     GLALACLERGAARQAGLFDDGDDRVARVERAGIEAARRGLGKLTRARLLHGPGPTRPK
                     PS"
     gene            complement(3583..4767)
                     /locus_tag="PLQ29_00610"
     CDS             complement(3583..4767)
                     /locus_tag="PLQ29_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85172.1"
                     /translation="MNVSKTHALILALTLSAALSSCVSTPATDARDADQATPLEGSAP
                     AAPAGEASSTKASASLDAELDALVAKARDAIDANHLSEGIKFYISALAKATNGGRRSR
                     ADEITGTLNAIGARLTIEPHESWLEPDGSQRSGSTRQAARGEGLQPAVYLYESYGYAK
                     SAVPDAFIRFEFVSNGGKLVASVATDAKGMANTSIGAIDSAGSDAVVRAFPIFTSEGY
                     SYAFRSVFRDFSYVAPPNLAIVAALERTPAGYSSNPRVLDAVGQSLKPLGVEVAPYNG
                     VLAPAGFEAAFAGDVRALASLAGTVKAGYFALVYVEVAAPTRMEYGGKVYNIYIANAK
                     ATLRIVRVDGTVVFAEAKDGVRGQGGTEQASIDDCLVKVRDELSAIISGKSEAIRKAL
                     AE"
     gene            complement(4778..5380)
                     /locus_tag="PLQ29_00615"
     CDS             complement(4778..5380)
                     /locus_tag="PLQ29_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85173.1"
                     /translation="MKRSTLLLAAATAILVSSCIGIDADARIAADGSVELTLAYTVSA
                     AVDELGKLGANADYLPLPVGRNDLELAASRAGGELRSWSREDGAESFAIKASLRFPSV
                     ASFAAFLDPAGEGAAYAEAGGRSTLTIRLSEGFDPADDDMLEFVKLAFADYAVNLRIT
                     APRTPTATSGLALSGRVATFSMKAADLYASNSPVTISLSW"
     gene            complement(5431..5709)
                     /locus_tag="PLQ29_00620"
     CDS             complement(5431..5709)
                     /locus_tag="PLQ29_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HPG85174.1"
                     /translation="MLQVIIEIPESFSLKDKRRVVKSVKDRLVSKFRVSCAETDLQES
                     LRFAQLGAALVSNSAEFGEGVMRKALAVIEGELSLRLHDSGIHSERFD"
     gene            complement(5718..6767)
                     /locus_tag="PLQ29_00625"
     CDS             complement(5718..6767)
                     /locus_tag="PLQ29_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85175.1"
                     /translation="MERVIEKTRRSGAVSVIASSTMDALAIVSIVFAFVAAGMAAAGA
                     SWRASAFMAIVGVLFDILFSYEFFLGAIGRPSPFPWLSGLSSALPLLLVSGPFLAGWV
                     GADLGSAAVRGYWLARSPLGGLSVVAALRLLRAARPFLIWRRSGDDETVSPSASRAAS
                     ARAASARAASARAASARAAILGIVVVLAGAAMADALLIPGLSASSASRHEAAMDEIAA
                     ATDDEAMIRAAKAAGAIALRHDGRTLLAATGPISPSRYAVLSRGSLEAWFDVSDEARA
                     RGTAAAIAALASLVAAAGYAATLYGLSPLRALARRATAPTRPDQTAFDGPGAPRRDAP
                     AGREELAGILGKRAP"
     gene            6792..10670
                     /locus_tag="PLQ29_00630"
     CDS             6792..10670
                     /locus_tag="PLQ29_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85176.1"
                     /translation="MIKKRANTAALVRIAALAALCLPWSRARAQALYSVLPEEPPSSL
                     FSTKLGDSEVEAFAQGFWEASILSAGAWSIDSGGSSFGANPPFQFMQTPDLYVFLSLR
                     RTWFFEAYVTNEAADNRFTLAFEGDDGDFIKFARLGNSGISMPSYPFMSFGSPDGSFG
                     AAMSASDAASGVSLDAMVRWDGLRWETRDFFGSSEMIETELAPGDDLRGRRFVLPSGG
                     LVSVELYEARTSGPRRLASDEYSASLASGVILLAAEPSGELTAAYVDSLGARASVTLY
                     AFAEDDDGDPVRRYNRYEARNLYALPDTASARQLFVRDLATGTVDTRFEVGRVGPGLV
                     QVLRSGSGPDPDDDGYMRPFVDLTPWSYDRSNGGGDATVASEGFAIVARSSETVEAIM
                     LGDGAVAGTISVYRDGVASSSFGYDEAGRTLTLSPPPRAGESIRVRYAVSTTDGADGA
                     LAFGVGARFPWLGQSWSTAIGGRWPLFGLGYETGDSMQPAWLGISAGVTGRAGVVDGI
                     AATGAAVPVAYTLEAMARYLRAGSRGLYRVVGMEDYGDSLWLEPFRQVSPEDGGITAS
                     VVVDAELAASDAFGDLLDDLRSGGGPTNRALEIVAGEAGSSSDARFVRYVDPVPLSSY
                     ERLSFFVKATGTGLGSVLTLRVGNGKGTGAVVSLPLGPIAGDGWRKVTMGLDPLAALA
                     IVSGDGESLVIAGASASYAAPDTAGLVELELSGLAAGTGAAVMIDELALEGARDGFSA
                     LAAASLSFGDRTEKRGAYLLAEASGELADAAVVASRLEAGLNAPFAELSLSASPAYAS
                     GQASAGLGYAVALPGRRSPWRVVDEYSVDEALGLYARELQAAARSGLFTASAVAGSSQ
                     EPNAFDQAWKASVGVAGVATATMAASLASSEAVVSGLGFGEAWLDSWSLLLPASEADA
                     DSRRLELAADAAGSRLTLSAYRDYERGSVPASSVVAKASLPTRFGPVSIAPYYSRKAS
                     VERASESGSFAGDVADFYRAVVAAGTLWSSVPIVELWSADAFDGFEAFADGASAASHE
                     AELGVALRRPIGYGLVDLLAPSSATLSYARLIELDADTAVESGRLSLSIAGGAANVFA
                     SSGAAPLLKRAAFDEYSFKHALSLTRYASDGAVLPSLTSSLAMSLEGTGGLGFALTSN
                     LAIETTRSLIPWSESIDAALTTRPTRTWLGDLAGLAIDARRRSVRASADSGADEEPSW
                     VSEWLDATLSEPLALKDGYTAGLTVGRTKSASAPLSERLSLGYSTRATAGGSLSIGLG
                     VGLWQTLAAYVDGALWGFGYSLSLEAKVVF"
     gene            10732..12297
                     /gene="metG"
                     /locus_tag="PLQ29_00635"
                     /pseudo
     CDS             10732..12297
                     /gene="metG"
                     /locus_tag="PLQ29_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968740.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
BASE COUNT         1851 a         4123 c         4223 g         2100 t
ORIGIN      
        1 cgttggccgc cagctccatc acgttgagcg ccacatactc ggctatcttg gctttttcca
       61 tgtattcggc gaggctcgtc cggatatcct tgatcagcgt gtcgtagccg tcggaatcgt
      121 ggaacttggc tatgatgaac caggtaaacg gcctgagcgt gttgaggaac ttctccgaca
      181 ggaggagctg tatgttcttc tcctcgggca ggaggttcga gttgcggttg atcagcgcgt
      241 acatcggggc gagcaccgtg cgcttgatct ccgcaatctc cttgtcctta tccttgatga
      301 aggcctggag gaagacgtcg ttaattttgg tcttctcgtc gatgatgttg gcggggttct
      361 tgcggttcca gttttttatg acgtcgctcg agagtacccg ctggaagata tagctgtcgt
      421 actggcggta catcatcgaa taggtcacca gcttggagat atccatcacc tcctggcgta
      481 tcttgacgaa ctcgctgtcc gatatctcga ccttcgatac gtagtcgatc agcatcatgc
      541 gctgcaggct ggcgggcgta aaatggtcga gcaggatgcc gtattcctcg acgttgtccg
      601 cgagtttgaa ttttcggagt ttcttgttgt tcttgatgaa ataggtggtg ccgctatcgg
      661 tcaggacgac cttgacgggc agttcgatga cggctttgcg ttcctgcgac cccatcctga
      721 ttcatacctc cgttgccgat ccgtcgttgc agtatatccc atcgtcgccc acgtgtaaaa
      781 cggtattgac ctggcgtcgg gctcgcccct aagatcgcca tacgacatgt ttcggcattt
      841 atcgatcgcc gtattagcca ttatcgcctc cggctcgctc gccagcctta cgctcggtgc
      901 gcaggaagcc tcgaacgacc cgaggacggt cgagatctcg ggcgcgttcg acctggtctc
      961 cgcccgggcg atcggcacgg gcggcgctac ggtggcgttc gtcgcggccg ctggcggcga
     1021 aggcggagcc gcgtccgagg cggccctgga cgcggccctg cacgccttgc gatccgcctc
     1081 ggtccacggg tacaccgcct acatcatgct gttcgaccca gagcccgccg cgatcccgcg
     1141 ggctagcgag ccgggagacc cgctgatacg ggagccgagc ctcgcccggg ccctcgccga
     1201 catggggatg gacgcggtcg tgctattgga cctcgggccg tccggcgggg cgatggggaa
     1261 tgggtcgatg caggggcccg cggtcagagc cgcgtccctc ggcatgatga cgccacggtc
     1321 cgtcctggag gcggtccgcg ttgcctcacg ggccgccgga ctgaggatcc gcgagtcgcc
     1381 cgtagaagat ttctacgctt cggccggcct gtcccccgga tcgaaggcgc tcgcaccctg
     1441 gctcgaggcc ggactcgcgg ccgtcgcgat cgaaagccgt ccggaggaca tcgaaacctt
     1501 cgcggcgctg gccccggcca tagccggcgc agtagccggc caggtcgcgg cgctcgtgtc
     1561 tgaggactcg gccgtgcccg gctccgacga cgtcggctac ctgcgctacc cggccccgac
     1621 cgggcccgtc ctcgtctccg acgcggccat cacgctgggc accatcacca cgacggccct
     1681 cgtcgcggcg gtcctggcgc tcggcctcct gaacggccga aggcgcaagg cttcgctcga
     1741 ggcagtggct aaggaagcgg cgggggcgtt cgcgctgtcg ttcatagcgc tgttcggcgc
     1801 cagggccctg tcgctcctgg cggcctcggc cgtccgggcc ctcggcgacc cgaccggccc
     1861 gggctctccg tcatgggccg tggccgtggc gctggtccta cgcgtggccg gaacgctcag
     1921 cctctactat actgcctctg gcctggcctc gaggctcggc ctgcacggcg accatcggcg
     1981 catcgacgcc gcgagggcgt cgctcgggct cctgtgcctt gacgcgctcg tggccgtcgt
     2041 ggtcttcccg ccggtcgcgc ccttcctcct ggcggccgtg atcgtctcgg cgctcgcgtc
     2101 ggaacgggcc gttggcgcgg ccatgggcct cgtggcggtg tcgctcctgg ccttgccgtt
     2161 cttcgacccg agggtgatag cggccatcgg ctcggctcga ggcgctgaga gcgtcgctca
     2221 ggccatgcta tccgccggac tgcgcggcaa cgccgtcgcc gcggctttcg cctcgccatt
     2281 cggtctatgg ctcaacgccg cctcgtcgcc agagacgagg ctcaggcgcg gcgccaggat
     2341 agcgccgctc tggctggtcg ccgccatcgt ctgcgctgtc ggcgaggccg tcctgacggc
     2401 ttcgctatga gcgactcgct atgacgagcg tctttttcca catcgacctt gacgcgttct
     2461 tcgccgcggc cgaggttctc gacgacccgt cgctcgcggg tcggcccgtc atcgtcggtg
     2521 cggccccggg tcatcgcggc gtcgtatcga cctgttccta cgaggccagg cgctacggcg
     2581 tccattcggc gatgcccatc tccgaggcct atcgcctctg ccccgaggcg gccttcagac
     2641 cggtcaggat ggagcgttac gctgagctat cggcccgcgt gatggcggtc ttcgaggcgt
     2701 acacgccgga cgtcgtccgc gtatccatcg acgaagccag cctcgatatg accggtaccg
     2761 agcgcctctg gggagcgccg cgggaggcag cggaagcgat acgcgatagg gtggcgcgcg
     2821 agaccggcct gtccatctcc atagggatag cgccgaaccg ttacgtggcc aaggtcgcct
     2881 ccggcgttcg gaagccggcc ggactggtcg tcgtcgaacc gggcgacgag gcggccttca
     2941 tggccggcct gcggctcaag gacctctggg gcgtcggccc caagaccagg gcgcggctcg
     3001 aggagctcgg catcggcgac caggcccggc tactcgaact atcgagggcg accctcgagt
     3061 cgaacttcgg caaggccggc ggcgagttca tctacggcgt cgcccgtggc gtcgacccgg
     3121 gcatctatgc cggcgaggcc aggtcacggt cgatcagctc cgagaccacc tacgagcgcg
     3181 acgaggccgg ccttcccagg atagagacga cgctgctcgg catggccgag gagctggtcg
     3241 ccaggctcta cgcggacggc tactcgtcgc gctgcgtcgc gctcaagctg cgctacgggg
     3301 acttcgagac ggtcggcgtc agggagacgc tcgacgagcc gttcttcgat tcgggggcgg
     3361 tctacgaggc ggcgcgctcc ctgctggcgc gcaagtggga cgggcggccg ctacgcctcg
     3421 tcgggttggc gctcgcctgc ctggagcgcg gagcggcgag gcaggcgggg ctgttcgacg
     3481 atggcgacga cagggttgcc cgcgtcgagc gggccggcat cgaggcggcg cgccgcgggc
     3541 tcggcaagct tacccgggcg cggctcctac acgggccggg acctactcgg ccaaagcctt
     3601 cctgatagcc tcggacttgc ccgagatgat ggccgacagc tcgtccctga ccttgaccag
     3661 gcaatcgtcg atcgaggcct gctcggtgcc gccttggccg cggacgccgt cctttgcctc
     3721 ggcgaacacg acggtgccgt cgacccggac gatgcgcagg gtggccttgg cgttggctat
     3781 gtagatgttg tagaccttgc cgccgtattc catcctggta ggcgcggcca cttcgacgta
     3841 gacgagcgcg aaatagccgg ccttgaccgt gccggccaac gaggccaggg cccgtacgtc
     3901 tccggcgaag gccgcctcga agccagcggg cgcgagcacg ccgttgtacg gagccacctc
     3961 gacgccgagc ggcttgagcg actggccgac cgcgtcgagc acgcgcgggt tggagctata
     4021 gccggccggg gtccgttcga gcgcggccac gatggcgagg ttcggcggcg cgacgtagct
     4081 gaagtcgcgg aacaccgacc tgaacgcgta gctgtagcct tcggacgtaa agatcgggaa
     4141 ggctctgacg acggcgtcgg aaccggcgga gtctatcgcg ccgatgctcg tgttggccat
     4201 gcccttggcg tcggtcgcga ccgacgcgac gagcttgcct ccgttggaca cgaactcgaa
     4261 gcggatgaag gcgtccggca cggcgctctt cgcgtagcca tagctctcgt agaggtagac
     4321 cgccggctgc aaaccctcgc cgcgagccgc ctgcctggtg ctaccggacc tctgcgaccc
     4381 gtccggctcg agccaggact cgtgcggttc tatagtcagc ctggctccga tcgcgttgag
     4441 cgtcccggtg atctcgtcgg ctctcgatcg cctgccgcca ttcgtcgcct tggcgagggc
     4501 ggatatatag aacttgatgc cctcggacag gtggttcgcg tctatggcgt cgcgggcctt
     4561 ggcgacgagg gcgtcaagct cggcgtcgag cgaggcggag gccttcgtcg agctggcctc
     4621 gccggctggg gccgccggcg cgcttccctc gaggggcgtc gcctggtcgg cgtcgcgggc
     4681 gtctgtcgcc ggcgtcgaga cgcaggacga aagggccgcc gagagggtta gcgcgaggat
     4741 cagggcatgc gttttggata cgttcatggc atcgctccta ccagctcagg ctgatcgtta
     4801 ctggagagtt cgaggcgtac aggtcggccg ccttcatgga gaaggtggca acgcgccccg
     4861 acagcgctag gccgctcgtg gccgtcggcg tccgaggtgc ggtaattctc aggttgacgg
     4921 cgtagtcggc gaaggcgagc ttgacgaatt cgagcatatc gtcgtcggcc ggatcgaagc
     4981 cctcggacaa cctgatcgtc agagtcgaac ggccgccggc ctcggcgtag gcggcgccct
     5041 cgccggcggg gtcgaggaag gccgcgaagg aggccacgct cgggaagcgt agcgaggctt
     5101 tgatcgcgaa cgactcggcg ccgtcctcgc gcgaccagga tcgtagttct ccgccggccc
     5161 gcgaggcggc cagctcgagg tcgttcctgc cgaccggcag cggcaggtag tcggcgttgg
     5221 cgccgagttt gccgagctcg tcgacggcgg ccgacacggt ataggcgagc gtcagctcca
     5281 cggagccgtc cgcggcgatg cgggcgtcgg cgtcgatgcc gatgcacgat gatacaagga
     5341 tggccgtagc ggcggcgagc aatagggtac ttcgcttcat gatagacaac cgtagctcaa
     5401 aatcgccccg ttgtcgagcg cgccgtcacg ctagtcgaag cgctcgctgt ggatcccgga
     5461 gtcgtggagc cggagcgaca gctcgccctc gatgacggca agtgccttgc gcatgacccc
     5521 ctcgccgaat tcggccgagt tcgatacgag cgcggcgccc agctgggcga agcgtagcga
     5581 ttcctgcagg tcggtctccg cgcacgagac gcggaacttg gacacgagcc tatccttgac
     5641 cgacttgacc acgcgacgct tgtccttgag gctaaacgac tcgggtatct cgatgatgac
     5701 ctggagcatg gagacgatca tggggcgcgc ttgccgagga tgccggcgag ctcctccctg
     5761 ccggccgggg cgtcgcggcg aggagcgcct ggcccgtcga acgccgtctg gtcggggcgc
     5821 gtaggagccg tcgcgcgacg tgccaacgcc cggagcggcg aaaggccgta gagcgtcgcc
     5881 gcatagccgg cggcggcgac gaggctggcc agggcggcta tcgcggccgc ggttccgcgg
     5941 gcgcgggctt cgtccgatac gtcgaaccag gcctcgagac tgccgcgcga caggacggcg
     6001 tacctggatg gagagatcgg tccggtcgcg gccaagagcg ttcggccgtc gtgtctcagg
     6061 gcgattgcgc cggcggcctt ggcggcccgg atcatagctt catcgtcggt cgcagccgct
     6121 atctcgtcca tcgcggcctc gtgccgcgac gccgaactcg ccgaaaggcc gggtatgagt
     6181 aatgcgtcgg ccatggcggc gcctgccagc acgacgacga tgcctaggat cgccgcgcgc
     6241 gccgacgctg cgcgcgccga cgctgcgcgc gccgacgccg cgcgcgccga cgccgcgcgc
     6301 gaagcgcttg ggctcaccgt ctcatcgtcg cccgatcggc gccatatcag gaagggcctg
     6361 gccgccctga gcaacctgag cgccgcgacg acagataatc cgccgagcgg ggaacgggcc
     6421 agccagtagc cgcggacggc cgccgagccc aggtcggctc ccacccagcc cgccaggaac
     6481 gggccggaga ccaggagcag gggcagggcc gacgacagcc cggacagcca tgggaagggc
     6541 gaaggacgtc cgatggcccc gaggaagaac tcgtacgaga ataggatatc gaacagcacg
     6601 ccgacgatgg ccatgaaggc cgaggcgcgc cacgacgcgc cggccgccgc catgcctgcg
     6661 gcgacgaacg cgaagacgat cgagacgatc gccaaagcgt ccatggtcga cgaggcgatg
     6721 accgatacgg cgccggagcg gcgcgtcttc tcaataacgc gttccatggg ctacaatgat
     6781 atacgcgaac gatgataaag aaaagagcga atacggcggc cctcgtccgc atagccgcgc
     6841 tggcggcgct gtgcctcccc tggtcccggg cgcgggcgca agccctgtac tcggtcctgc
     6901 ctgaggagcc gccatcgagc ctgttctcga ccaagctcgg ggactcggag gtggaagcct
     6961 tcgcccaggg cttctgggag gcgtcgatcc tgagcgccgg cgcctggtcg atagactccg
     7021 gcggatcgag cttcggtgcc aatccgccgt tccagttcat gcagacgccc gacctatacg
     7081 tattcctgag cctcaggcga acatggttct tcgaggccta tgtgacgaac gaggccgcgg
     7141 ataatcggtt cacgctggcc ttcgagggcg acgatgggga tttcatcaag ttcgcccggc
     7201 tcggcaactc gggcatatcg atgccgtcct atccgttcat gtcgttcggc tctccggacg
     7261 gctctttcgg cgccgcgatg agcgccagcg acgcggcgag cggcgtgtcg ctcgacgcga
     7321 tggtccgctg ggacggtctc cgctgggaga cccgggactt cttcggttcg tcggagatga
     7381 tcgagacgga gctggcccct ggcgacgacc tgcgcggccg gcgtttcgtg ctgccttccg
     7441 gcggcctcgt ctcggtcgag ctgtacgagg cgcgcacgag cggcccgagg cgattggcta
     7501 gcgacgagta ctcggcctcc ctggcctcgg gcgtcatcct gctcgccgcg gagccgtcgg
     7561 gcgagctgac ggcagcctac gtcgattcgc tcggcgctcg ggcgagcgtc acgctctacg
     7621 ccttcgcgga ggatgacgac ggcgacccgg tacgacgata caaccgctac gaggcgcgca
     7681 atctgtacgc gctaccagac acggcctcgg caaggcagct gttcgtccgc gacttggcca
     7741 ccggtaccgt cgatacccgc ttcgaggtcg gtcgcgtcgg cccgggcctg gtacaggtcc
     7801 tgcgctcggg atcgggaccg gatcccgacg acgacggata catgcggccg ttcgtcgacc
     7861 tgactccgtg gagctacgat aggtcgaacg gcggcggcga cgcgacggtg gccagcgagg
     7921 gcttcgccat cgtcgccagg tcatcggaga cggtcgaggc aatcatgctc ggcgacggcg
     7981 ccgtggctgg gacgatctcg gtctaccgtg atggggtcgc ttcgtcatcg tttggctatg
     8041 acgaggcggg ccgaacactc acgctatccc cgccgccgag ggccggcgag agcatacggg
     8101 tgcggtacgc cgtgtcgacc acggacgggg cagacggagc gctcgccttc ggcgtcgggg
     8161 cgcgctttcc ctggctcggc cagagctggt cgacggccat aggcggccgc tggccactct
     8221 tcggactcgg ttacgagacc ggcgactcca tgcagccggc ctggctaggc atatcggccg
     8281 gcgtcaccgg ccgagccggc gtcgtggacg gaatcgccgc aacgggcgcg gccgttccgg
     8341 tggcgtatac cctcgaggcg atggcacgct acctgcgcgc cggttcccgg gggctgtacc
     8401 gggtggtcgg catggaggat tacggcgact cgctctggct cgagccgttc aggcaggtat
     8461 cgccggagga cggcggcata acggcctccg tcgtcgtcga cgccgagctg gccgcgtccg
     8521 acgccttcgg cgacctgctc gacgacctgc gctccggcgg cggtccgacc aaccgcgcgc
     8581 tcgagatcgt cgccggagag gccggatcgt cgtcggacgc ccgcttcgtg cgctacgtcg
     8641 atccggtgcc cctgtcctcg tatgaacggc tgtcgttctt cgtcaaggcg acggggacgg
     8701 ggctcggctc ggttttgaca ctgcgcgtcg ggaatggtaa gggaactggc gccgtcgtca
     8761 gtctgccgct cggccctata gccggcgacg gctggcgcaa ggtgacgatg gggctcgatc
     8821 cgttggccgc actggctatc gtatccggcg acggtgagtc gctcgtcatt gccggcgcct
     8881 ctgcctcgta cgcggcgccg gacacggccg gactcgtcga gctcgagcta tccggcttgg
     8941 cggccgggac cggcgccgcg gtcatgatcg acgagcttgc gctcgagggc gcccgcgacg
     9001 gtttctcggc gttggcggcc gcgagcctat cgttcggcga ccgcacggag aagcgcgggg
     9061 cgtacctgct agccgaggcg tccggtgaac tggccgatgc cgccgtggtg gcttccaggc
     9121 tcgaggcagg cctgaacgcg cctttcgccg agctgtccct aagcgcctcg ccggcctatg
     9181 cttctggcca ggcctcggct ggccttggct acgcggtcgc gctacccggc cggcgttcgc
     9241 catggcgcgt cgtcgacgag tactccgtgg acgaggcgct cggcttatac gcccgcgagc
     9301 tccaggcggc ggctcgctcg ggtctgttca ccgcgagcgc cgttgcagga tccagtcagg
     9361 agccgaacgc gtttgaccag gcctggaagg cgagcgtcgg cgtcgcaggc gtcgcgacgg
     9421 cgaccatggc ggcatcgctc gcctcgtcgg aggccgtcgt ctccggactc ggtttcggcg
     9481 aagcctggct cgattcctgg tcgctcctac taccggcctc cgaggccgac gccgattcca
     9541 ggcgcctgga actggccgcc gacgccgcgg gctcgcgctt gactctgtcg gcttaccgcg
     9601 actacgagcg cggttccgtt cccgcgtcgt ccgtggtcgc caaggcgagc ctgcctacgc
     9661 ggttcggccc cgtgtcgatc gctccgtact attctcgcaa ggcgagcgtg gagcgcgcca
     9721 gcgagtcagg ctccttcgcc ggcgacgtag ccgacttcta ccgggccgtg gtggccgctg
     9781 ggactctctg gtcctccgta cccatcgtcg agctgtggtc ggccgacgcc ttcgacggct
     9841 tcgaggcgtt cgccgacggc gcctcggccg cctcgcacga ggccgaactc ggcgtcgcgc
     9901 tacgccgacc gataggctac ggcctcgtcg acctgctggc cccgagctcc gcgaccctgt
     9961 cctacgcgag gctcatcgag ttggacgccg acacggccgt agagtccggc cggctgtcgc
    10021 tatccatcgc tggaggggcg gccaacgtgt tcgcctcgag cggggccgcg ccgctcctca
    10081 agcgcgcggc gttcgacgag tactcgttca agcacgcgct tagcctgacc cgatacgcct
    10141 ccgacggcgc cgtcctgccg tcgctgacgt cgagcctggc gatgagcctc gagggtacgg
    10201 gcggcctggg cttcgcgctg acctcgaacc tggccatcga gacgacgagg agcctcatcc
    10261 cgtggtccga gtcgatcgac gcggcgctga cgacgcgccc gactcgcacc tggctcggag
    10321 acctggctgg cctggccatc gacgcgagga ggcgctcggt ccgggcctcg gcggactctg
    10381 gcgccgacga ggaaccgagc tgggtgtcgg aatggctcga cgcgacgctc tccgagccgc
    10441 tcgcgctcaa ggacggatat accgccggcc tgaccgtcgg gcggacgaag agcgcctcgg
    10501 cacccttgtc cgagcgcctc tcgctcggct actcgaccag ggccacggcc gggggaagcc
    10561 tttccatcgg cctcggagtc gggctgtggc agacgctcgc ggcctacgtc gacggcgcgc
    10621 tctggggctt cggctactcg ctatcgctcg aggccaaggt agtcttctga gcgcctatcg
    10681 gcgggtaccg ccgttgacag cggtttttac cctccgtata gtaaggccac catgaaacga
    10741 cgcctcgtaa ccagcgcact cccgtacgtc aataacgttc cgcacctcgg caacctgatc
    10801 caggtcctgt cggccgacgt gttcgcccgc gcctgtcggc tccgcggcta cgagaccctc
    10861 tacgtctgcg gcaccgacga gtacggcccg gccaccgaga ccaaggcggc cgaggagggc
    10921 gtgagccccg ccgagctgtg ctcgcgcttc cacgagatcc ataaggccat ctacgaatgg
    10981 ttcggcatag gcttcgataa attcggccgg acctcgacgc ctcgccagaa ggagatcgtg
    11041 cagtcgctgt tcaaggatct cgacgaggcc ggtttcatca ccgagcagac catcgagcag
    11101 ctgtactgcg agccgtgcgg ccgcttcctg gccgaccgct acgtacgcgg cacctgcccg
    11161 cactgcggct acgacgacgc gcgtggcgac cagtgcgagg cctgcggcaa gctactcgag
    11221 ccctcggaac tccagcggcc caagtgctcg acctgcgggg cctcgccgaa gccgcgctcg
    11281 accaggcacc tgtacatcga cctgccggcc atcaagccca ggctcgaggc ctggatgaag
    11341 gacgcgagcg tcaagggctt ctggaccaat aacgccatcc agatgaccca ggcctggata
    11401 cgcgacgggc tcaaaccgcg cgccatcacg cgcgacctca agtggggcat cccggtgccc
    11461 aagccgggct tcgaggacaa ggtcttctac gtctggttcg acgcgcccat cggctacctg
    11521 tccatctccg cctgcggggg cgacgagcgc ggcttcgact ggaaggactg gtggatgaag
    11581 cccgacgagg tggagctgtt ccagttcata ggcaaggaca atatcccgtt ccacacggtg
    11641 atcttcccga gcaccctgct cggttccggc cgggattgga cgatgcttca ccatatgtcc
    11701 agcaccgagt acctgaacta cgagtcgagc aagttctcca agtccaaggg cgtcggcgtc
    11761 ttcggcaccg acgcgatgga gaccggcata ccggccgatg tctggcgctt ctatgtcttc
    11821 tataatcggc ccgagaagag cgacgtggtc ttcacctggg ccgacttcct cgagaaggtg
    11881 aacggcgagc tcatcggtaa cctgggtaac ctggccaacc gtacgctgtc gttcgtgcaa
    11941 cgcttctacg acggcgtcat accggtcggc aagccggacg aggccttcta ggccgaggtg
    12001 cgcgccctcg aggccgaggt tgccgatagg ctcgagcgcg ccgagctgcg cgacgcgttc
    12061 aggacggcct tccagatctc ggacatcgcc aacaagcgct tccaggccga agagccgtgg
    12121 aaggccagga cggccgaacc cgagaaggcc gcgagcctcc tctccgacct ctgcctcgtc
    12181 ctccgcgacc tcgcggtgct catccacccc tacatgcccg cggccgcgga aaagctcgcc
    12241 ggcttcttcc gcaagaccgt gggcggggcc tcggggctat cgtggaagga cctcgcg
//
LOCUS       DAOPHV010000011        12131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_6246_length_12131_cov_4.306641, whole genome shotgun sequence.
ACCESSION   DAOPHV010000011 DAOPHV010000000
VERSION     DAOPHV010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 12131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12131
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6246_length_12131_cov_4.306641"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1148
                     /gene="floA"
                     /locus_tag="PLQ29_00640"
     CDS             159..1148
                     /gene="floA"
                     /locus_tag="PLQ29_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527462.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HPG85177.1"
                     /translation="MLTVALYALVGLVALGVLFMFFKFFGLWFRALLSGAPVGMGRLI
                     GMWLRHVKPGIIVDARIMLSKAGLDVNSDMLETHFLARGNVFKVSQALVAANKADIPL
                     PFQRAAAIDLAGRDVLEAVKTSVNPKVIDCPDPGKGKNTIDAVAKDGIQLQVKARVTV
                     RANIERLVGGATEETIIARVGEGIVTTIGSSESYKSVLENPDLISKRVLGKGLDAGTA
                     FEILSIDIADIDVGANVGAKLQADQAEADMRRFQAEAEKRRAAAQAREQEMLALVQEN
                     RAKVVLAEAEIPLAIAEAFKKGQLGIMDYYKLTNIQADTKMRSSIGGEAPSGA"
     gene            1150..1611
                     /locus_tag="PLQ29_00645"
     CDS             1150..1611
                     /locus_tag="PLQ29_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85178.1"
                     /translation="MEELLENVYVVIPIALLIFARLFADRLRAKRAGEAGATKATAGT
                     RGPGALARFLGRLSGRVPEPSPSPRTRSEPLHFEEPVFRLGERLPPPAAAPIEPHTLM
                     APAPAAKRPGPARPTGAPPTSARLPSSVERLSPLRKAVVMAELLGPPKALR"
     gene            complement(1616..1924)
                     /locus_tag="PLQ29_00650"
     CDS             complement(1616..1924)
                     /locus_tag="PLQ29_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HPG85179.1"
                     /translation="MSDDIGASAPRDDDPLHGVTLRMMVERLSERIGWAGLAEKVPVR
                     CFTNDPSVDSSLKFLRRTPWAREKVELLYLYAFHPKRARELAVKRRGGERPGRLDPRR
                     "
     gene            2058..3095
                     /locus_tag="PLQ29_00655"
     CDS             2058..3095
                     /locus_tag="PLQ29_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HPG85180.1"
                     /translation="MQNEIVSPMDLLDDSGRITREGWARYPYWRYDRAKIRSNALRIK
                     EWDYYSVLSQDGRFGIALTMSDLGYAGLFAICFLDFERRTSHQVDTLSVLPLGRTGFP
                     PVSDEGRISFGDKKLSMTFVYAGGVRTLSFSAPGLKNAEGDTGLEGAIILTQPPELES
                     VNIATSWAENRRAFYYNRKLNCMKASGGFTIGRKRYEFDPAKDFGELDWGRGRWTYKN
                     RWYWGSAAGLAGGVPFGWNIGYGFSDRSPASENAVFYAGKVHKLDEVTFHIDTADYLA
                     PWRFSSSDGRFEMDFSPLVDRSSTMAIGPIKSVQHQVFGEFTGTAILDDGQAIRADRL
                     LGFAEDVLNWW"
     gene            complement(3102..3695)
                     /locus_tag="PLQ29_00660"
     CDS             complement(3102..3695)
                     /locus_tag="PLQ29_00660"
                     /inference="COORDINATES: protein motif:HMM:NF017466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HPG85181.1"
                     /translation="MVASNTASIDIFERIVSLVHEATGHGVNIMGDGGRILASSDPTR
                     VGTVHDGGRRVMSGEVEEVAIDADTAATLRNVKAGYMGAVRMDGKLIACIGIGGEPHE
                     VKPLQKMAALSLKQELERERLAGRERGLLQDVRRDIGDIAERMQVLSLNGAVLAARLG
                     EKGRGFKIVVGEMRELAAQIGEKLVELERREKNVVSE"
     gene            complement(3771..5207)
                     /locus_tag="PLQ29_00665"
     CDS             complement(3771..5207)
                     /locus_tag="PLQ29_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HPG85182.1"
                     /translation="MSDAANAEAIFKAAVDRVDPSRMIERAVRIERDDLVVSAGAESV
                     RYGLGGIDRIFVTGMGKASAKMAAGLERALGSRIAGGLVAVKEGHVEPLERVRLVEAG
                     HPVPDGRSQAAAAAILGLGSSLGVRLTERDLVIVLVSGGGSAILCAPAPGLTLEDKIG
                     TTRLLLASGATINEVNCVRKHLSAVKGGRLAAALAPASVLALVLSDVIGDDLDAIASG
                     PTVPDPTSYADALEVVRRYGIESRLPASVAERLRAGAAGAVGGPGGPGDPGDPGDPGD
                     PGDPGDPGAPTETPKADDPAFARTRTLLVGTNRLALAAAEAEARRLGYNSLVVSSRVS
                     GEAREIAMAFLGIGKDIAASDFPVAKPACVIAGGETTVTLRGGGKGGRNQEMALAFMA
                     ALGRSPKDGERLTFLSAGTDGNDGPTEAAGAVVSLGLYRSAVAAGLDPEAALADNDSN
                     GFFRKAGGLVVTGPTNTNVCDVQVLIVT"
     gene            5409..7085
                     /locus_tag="PLQ29_00670"
     CDS             5409..7085
                     /locus_tag="PLQ29_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPG85183.1"
                     /translation="MTACQFEKIVQASHDFITLIDRDYRYAFVNDSYARELGLPAIDI
                     VGKTVGEIWGDEKFRRRIKPRIDQCFRGDESHDVDKFTFGSDSKYIHVSYYPYGDDGE
                     TSHVMVFSHDISIVKKLESKLLDFEFKDPTTGLFNRRSFDIVLDMELEKARRSGAHGV
                     RAVLFIKLRSISHINAAFGYELGNLLLESTALRIKDALRASDYVFRFDGKEFAVILTT
                     IKRGADIPIVADNVRGKTNFPYSYGGTVINVSCNIGAAVYPSDGDAREVIVSRAMAAM
                     NEAHERDEPLVMFNKELFERGRYVSKLRADFHAAFIERQFTTWFQPIVDAGGMIVGAE
                     ALIRWHHPTLGNVPPSVFIPIAEESGNIAMIGKWVLFQVCRDIRRFGDALGERYISVN
                     LTTAEFSRPTLVDEIRELLETERIRPASLKLEITESQSMKDVDQVIRKIEDLSDIGID
                     VLIDDFGTGYSSLAYLKRLPAKTIKVDKAFVDHIAESDEELEFIRGVMTMIESKKKNV
                     LVEGVTSLNQYKLLRGLGAAYLQGYYFSEPRSGDDFLALLKAGKPLPVSA"
     gene            complement(7126..7911)
                     /locus_tag="PLQ29_00675"
     CDS             complement(7126..7911)
                     /locus_tag="PLQ29_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85184.1"
                     /translation="MNDEKHLYLIMHPNHALIASQLDPERFAKHYTQGSTRYFEGRLI
                     FVEIDPSFRHPYFNIDAAFAELKPHEDGRPKATKFISSYRTFEHMDFNAFGKLYYCNS
                     LGDYVELESAAYDPKMRGDEMRIVLEINPIKMMVLTKYNFLEYAKFITDPDMPKGAPV
                     MFYTQLEFTVDEFLKEFQENPFIRCFVPGIHPARLREAIFEVRAKPGKNTKGLSLDCP
                     VDRISYKFLRHGFMFASATQTKFYPLMSLEDAERKYYKFWKNM"
     gene            complement(7916..9988)
                     /locus_tag="PLQ29_00680"
     CDS             complement(7916..9988)
                     /locus_tag="PLQ29_00680"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPG85185.1"
                     /translation="MNIIAVLLAAAIGGTIAIVYAVAKSRWIRKRSVGDATLERIAGY
                     IAEGAEAFLRREYTVLVPFVALVALFLAVANSGSLRLEALAFVLGALCSAAAGYFGMR
                     TATAANSRTASAASDGIGPALRVAFAGGSVMGMTVVGLALLGIAAVVGLGVALFGASI
                     GALRDTIFPILSGFSLGASTIALFARVGGGIFTKAADVGADLVGKVEAGIPEDDPRNP
                     AVIADNVGDNVGDVAGMGADLFESYVGSIVGCVVLGIGVAASDDMRLRLALLPILMAA
                     MGILASIAGTFLVRGKPGRSPQKSLNAGSFGAAALAAALMYPLARLALGSGTFDGGRN
                     ALSVALAAVFGLVSGTAIGLLTEFFTGTNTAPVRKIERSCDTGAATAIITGLGVGMLS
                     TLPPVLVICGAMLGSYSIAGLYGIGMAALGMLVTLGIQLSVDAYGPIADNAGGLAVMS
                     AMDPHVRDVTDELDAVGNTTAAVGKGFAIGSAALTAIILFSAFAQSAGGSNALVFDVT
                     APKVLVGLLLGGMIPFLFSALSMDAIGASAFAMIEEVRRQFRERPGILEETETPDYRA
                     CVDISTKASLRRMILPGVIAAASPVVVGFAGGIEMLGGLLAGVTVTGVLLAIFMSNSG
                     GAWDNAKKLIESRAHGKGSDAHKAAVVGDTVGDPFKDTAGPALNILIKLMAIISLVIA
                     PMLKSYWG"
     gene            10184..11101
                     /locus_tag="PLQ29_00685"
     CDS             10184..11101
                     /locus_tag="PLQ29_00685"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPG85186.1"
                     /translation="MRHSAHTGALVRGAVTAASTLIVALVVGCGAERPDDGTLPVVVV
                     SIEPQAYFVERIAEGRVSVSVLVGPGQSPHSYEPTPRQMADLSAASMWLCVGAEFEKS
                     LRPKAAALYKSLPIVDTTTGVQYRKLEAHAHDDGDGDHDEEGGLDPHVWLGRQAVKAM
                     AANIRDALAAADPAGEDAYAANHDAFVRDADAAYDELASALAGLRGTTVFVYHPAFGY
                     LLDEFGIAQEAVETGGKEPTQKALAAMIKAAREDGASLIFVQAQFPTSAAEAVAEAIG
                     ATVVPIDPLARDWLDNLRRIGDELARMGP"
     gene            11098..11904
                     /locus_tag="PLQ29_00690"
     CDS             11098..11904
                     /locus_tag="PLQ29_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HPG85187.1"
                     /translation="MTMERSVAIRFESVSFSYPAARVLEAASFHVHAGEFAALVGPNG
                     AGKTTALRLILGLVRPESGRVTVLGRSPEEARNAIGYVPQHASYDPAFPISVSEVVRM
                     GRLRSSTRRYGPEDGRAVERALALADVEALASRPYPALSGGQRRRVLVARALAAEPEL
                     LILDEPTANMDSESEGRLFETLGGLKGQATILIVTHDTGFVTSLTDAVFCVGEGGSGG
                     RIVKHRAVPAAHAASVQYGGSALQVLHDTALPDNGCCSDDRVGHPERNAR"
     gene            11901..>12131
                     /locus_tag="PLQ29_00695"
     CDS             11901..>12131
                     /locus_tag="PLQ29_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPG85188.1"
                     /translation="MNGYIQALLDPTMPFVRNALAAGLLSAVLFGVLGAVVTVKRIAG
                     LAGAIAHAVLGGIGLSLFLAPKILPDGTRNDFR"
BASE COUNT         2113 a         4110 c         3954 g         1954 t
ORIGIN      
        1 caccctccgg ccgtcgggcc gcgccatcat cgacggcggg acctacagcg tcgaaacgga
       61 cgggacctac gtcgaggcgg gcgccgcgct ccgcgtcgac tcggtccgcg gctcgaggat
      121 actcgtatcg gtacgaacgc cagacaagga gtcgtgacat gctgaccgta gccctgtacg
      181 cgctcgtggg cctcgtcgcc ctcggcgtcc tgttcatgtt cttcaagttc ttcggcctgt
      241 ggttcagggc cctgctgtcc ggagcgcccg tcggcatggg ccgcctgatc ggcatgtggc
      301 tcaggcacgt caagccgggc atcatcgtcg acgcgcgcat catgctctcg aaggctggcc
      361 tcgacgttaa ttccgacatg ctggagacgc atttcctcgc gaggggcaac gtattcaagg
      421 tcagccaggc cctcgtcgcg gccaacaagg cggacatccc cctgccattc cagcgcgcgg
      481 ccgcgataga cctggccggc cgcgacgtgc tcgaggcggt caagaccagc gtcaacccca
      541 aggtgataga ctgccccgac cccggcaaag gcaagaacac gatagacgcg gtggccaagg
      601 acggcatcca gctccaggtc aaggcccgcg tcaccgtgcg cgccaacatc gagcgcctcg
      661 tcggcggcgc caccgaggag accatcatcg cccgcgtcgg cgagggcatc gtcacgacca
      721 tcggctcctc ggagtcgtac aagagcgtac tcgagaaccc ggacctgatc tccaagcgcg
      781 tgctcggcaa gggcctggac gccggcaccg ccttcgagat cctgtccatc gatatcgccg
      841 atatagacgt cggcgccaac gtcggcgcca agctgcaggc cgaccaggcg gaggcggaca
      901 tgcgccgctt ccaggcggag gcggagaaac gacgggcggc cgcccaggcc agggagcagg
      961 agatgctggc gctcgtgcag gagaaccgcg ccaaggtggt gctcgccgag gccgagatac
     1021 cgctcgcgat cgccgaggcc ttcaagaagg gccagctcgg tatcatggac tactataagc
     1081 tcacgaatat ccaggccgac accaagatgc gctcgtccat cggcggcgaa gcgccgtcgg
     1141 gcgcctgaca tggaagagct gctcgagaac gtctacgtcg tcatcccgat agcgctactg
     1201 atcttcgcgc ggctgttcgc cgataggctc agggcgaagc gcgccggcga agccggcgcg
     1261 acgaaggcca ccgccggaac gcgagggccg ggggcgctcg cgcggttcct gggccgcctc
     1321 tccggccgcg ttcccgagcc gtcgccgtcg ccgcgaacga gatcggaacc gctccatttc
     1381 gaggagcccg tcttccgtct cggcgagcgc ctgccgcccc cggccgccgc gccgatcgag
     1441 ccgcatacgc tcatggcgcc agctcccgcg gcgaagcggc ccgggccggc ccggccgacc
     1501 ggtgccccgc cgaccagtgc ccgactgccg tcctccgtcg aacggctatc gcccctgcgc
     1561 aaggccgtcg tcatggcgga gctgctcggc ccgcccaagg cgctcaggta ggtcgtcagc
     1621 gccgaggatc gagacgcccc ggacgctccc cgcctcgccg cttgaccgcc agctcgcggg
     1681 cccgtttcgg atggaaggcg tacaggtaga ggagctcgac cttctcgcgg gcccagggcg
     1741 ttcgccgcag gaacttgagg ctcgagtcga cgctcggatc gttcgtaaaa caacggaccg
     1801 gcaccttctc cgcgaggccg gcccagccga tacgctcgga caggcgctcg accatcatgc
     1861 gcagggtaac gccgtggaga gggtcgtcgt cccgcggcgc ggacgcgcca atatcgtctg
     1921 acacggaatg ctccttacgg cgctcgcggg cgccagagca cactatacct cgctacggcc
     1981 ggccgacgcc aggcgtttta cgctggcaaa gccggcgaac gcgtcgtata gtagtcgcat
     2041 cgacggaggc cggcgacatg cagaacgaaa tcgtttcacc gatggaccta ctcgacgact
     2101 cgggcagaat cacccgagag ggctgggccc gctaccccta ctggcgctac gaccgcgcga
     2161 agatacgctc gaacgcgctg cgcatcaagg aatgggacta ctactcggtc ctgtcgcagg
     2221 acggccgctt cggtatagcc ctgaccatga gcgacctggg ctacgccggc ctgttcgcga
     2281 tctgcttcct cgacttcgag cggcgcacgt cccatcaggt cgacacgctc agcgtgctac
     2341 cgctcggccg caccggcttt ccgccagtat ccgacgaagg caggatttca ttcggcgaca
     2401 agaagctgtc gatgaccttc gtgtacgccg gcggcgtgcg caccctatcg ttcagcgctc
     2461 ccgggctcaa gaacgccgaa ggcgatacgg gcctcgaggg cgccatcatc ctgacccagc
     2521 cgcccgagct cgaaagcgtc aacatcgcca cctcctgggc cgagaaccgc cgcgcgttct
     2581 actacaaccg caagctcaac tgcatgaagg cctcaggcgg tttcacgata ggcaggaagc
     2641 gctacgagtt cgaccccgcc aaggacttcg gggagctgga ctggggacgg ggccgctgga
     2701 cctacaagaa ccgctggtac tggggctcgg ccgccgggct cgcgggcggc gtacccttcg
     2761 gctggaacat cggctacggc ttctccgacc gcagcccggc cagcgagaac gccgtcttct
     2821 atgccggcaa ggtccataag ctcgacgagg tgaccttcca tatcgatacg gccgactacc
     2881 tcgccccctg gcgcttctcg agttccgacg gccgtttcga gatggacttc tcgccgctcg
     2941 tcgaccgtag ctcgaccatg gccatcggcc cgatcaagtc ggtccagcac caggttttcg
     3001 gcgaattcac cggcacggcc atactcgacg acggccaggc gatccgggcg gaccgcctcc
     3061 tcggcttcgc ggaggacgta ctcaactggt ggtaggcgtc gtcactcgga gacgacgttc
     3121 ttctcccggc gctcgagctc gacgagcttc tcgcctatct gggcggcgag ctcgcgcatc
     3181 tccccgacga caatcttgaa gccgcggccc ttctcgccga gcctcgcggc gagcaccgcg
     3241 ccgttcaggg acagcacctg catgcgctcg gctatgtcgc cgatgtctcg ccgcacgtcc
     3301 tggagcaggc cgcgttccct accggcgagc cgttcccgct cgagttcctg cttgagcgat
     3361 agcgcggcca ttttctggag cggcttgacc tcgtgaggct cgccgcctat gccgatgcac
     3421 gctatcagct tgccgtccat gcgaaccgcg cccatgtagc cggccttgac gttacgcagg
     3481 gtagctgcgg tgtcggcgtc tatggcgacc tcctcgacct cgccgctcat cacccgccgg
     3541 ccgccgtcat ggacggtacc gacgcgcgtc ggatcggagg aggccaggat gcggccacca
     3601 tcgcccatga tattgacgcc atgaccggtg gcctcatgaa cgagcgagac gattcgttcg
     3661 aatatatcga tagaagccgt attggacgcg accattcagg ttccttaagc gctaggatac
     3721 gcgcaatgta ccatgtatac acgtagtgct caaccaggat tcttagcgga ctaggtgacg
     3781 atgaggacct gtacgtcgca gacgttcgtg ttcgtcggac cggttacgac gaggcccccg
     3841 gctttcctga agaagccgtt ggagtcgttg tcggcgagcg ccgcctccgg atccaggccg
     3901 gcggcgacgg ccgagcggta caggcccagg ctaaccacgg cgcccgcggc ctcggtcggc
     3961 ccgtcgttgc catcggtgcc ggcggacagg aaggtcagcc gctctccgtc cttgggcgag
     4021 cggccgagcg cggccatgaa ggcgagcgcc atctcctggt tacggccgcc cttgccgcct
     4081 ccgcgcaggg tcacggtcgt ctcgccgcct gcgatgacgc aggccggctt ggccaccggg
     4141 aaatcggacg ccgcgatgtc cttgccgatg cccaggaacg ccatggcgat ctctcgggcc
     4201 tcgccagaaa cgcgcgacga cactacgagg ctgttgtagc cgagccgccg ggcctcggcc
     4261 tcggccgccg cgagcgccag ccggttcgtg ccgacgagca gggttctcgt gcgggcgaag
     4321 gccgggtcgt ccgccttggg cgtctcggtc ggcgcgccag gatcgccagg atcgccagga
     4381 tcgccaggat cgccaggatc gccaggatcg ccaggaccgc caggaccgcc gaccgccccg
     4441 gccgccccgg cccgcagccg ctcggcgacc gacgccggta gcctagactc tattccgtag
     4501 cgcctgacga cctcgagggc gtcggcataa ctcgtcggat ccggtaccgt cggcccggag
     4561 gctatggcgt cgagatcgtc gcctatcacg tctgacagga cgagggcgag caccgaggcc
     4621 ggcgccaggg ccgccgcgag acgcccgccc ttgaccgccg acaggtgctt gcgcacgcag
     4681 ttgacctcgt tgatcgtcgc gccggacgcg aggaggagcc gcgtcgttcc gatcttgtcc
     4741 tcgagcgtca gaccgggagc cggggcgcac aggatcgccg agccgcctcc cgagaccagg
     4801 acgatgacga ggtcgcgctc ggtcagcctg acgccgaggc tggagccgag gccgagtatc
     4861 gcggccgccg cggcctggga gcgcccatcg gggacggggt gccccgcctc gacgaggcgc
     4921 acgcgctcga gcggctcgac gtgaccttcc tttaccgcga cgagcccgcc cgctatcctc
     4981 gagccgagcg cccgctcgag acccgcggcc atcttggccg acgccttgcc catgcccgtg
     5041 acgaagatcc ggtcgatgcc gccgagcccg tagcggaccg actcggcgcc ggcggagacg
     5101 acgagatcgt cccgctctat ccgtacggcg cgctcgatca tccgcgaggg atccactctg
     5161 tctaccgccg ccttgaaaat cgcctcggcg ttcgccgcgt cgctcatgtc gatatcctcg
     5221 tcatcttggg ttcgagccgt accgggaacc atcgccgcgt cagccatcgg gccgacgggc
     5281 gccccgcccc tcgctcgatg ataccggatt accgaggata tcgacagccc tgccataggc
     5341 tgatccccga ccggtatggc atttcgcctt cctcgaaact atacttatag caccaatcga
     5401 ggtatagaat gacagcctgc caattcgaga agatcgtcca ggcgtcgcac gacttcatca
     5461 cgctcatcga ccgggactac cgctacgcct tcgtcaatga ctcctacgcc cgcgagctgg
     5521 ggctaccggc gatcgacatc gtcggcaaga cggtcggcga gatctggggc gatgagaaat
     5581 tcaggcggcg catcaagccg cgcatcgatc agtgcttccg cggcgacgag tcccacgacg
     5641 tcgacaaatt caccttcggg agcgactcca agtatatcca cgtctcctac tacccgtacg
     5701 gcgatgatgg agagacctcg cacgtgatgg tcttctcgca cgacatctcc atcgtcaaga
     5761 agctcgagtc caagctgctc gatttcgagt tcaaggaccc gacgaccggg ctcttcaatc
     5821 gacgttcgtt cgacatcgtc ttggacatgg agctggagaa ggccaggcgc tcgggagccc
     5881 acggcgtacg cgcggtactg ttcatcaagc tgaggagcat ctcccatatc aacgccgcgt
     5941 tcggctacga gctcggcaac ctgctcctcg agtcgaccgc gctcaggatc aaggacgcgc
     6001 tacgggccag cgactacgtg ttccgtttcg acggcaagga attcgccgtg atcctgacga
     6061 ccatcaagcg cggcgcagat ataccgattg tcgccgacaa tgtcaggggc aagacgaact
     6121 tcccctacag ctatggcggc acggtgatca acgtgtcctg caacatcggc gccgccgtgt
     6181 acccctccga cggcgacgcg cgcgaggtta tcgtatcgag ggcgatggcc gcgatgaacg
     6241 aggcccacga gcgcgacgag cccctggtca tgttcaacaa ggagctgttc gagcgcggca
     6301 ggtacgtgtc gaagctgcgc gccgacttcc acgccgcctt catcgagcgc cagttcacga
     6361 cctggttcca gcccatcgtc gacgcgggtg gcatgatagt cggtgccgag gcgctgatcc
     6421 gctggcatca cccgacgctc ggcaacgtgc cgccgtcggt cttcatcccg atcgcggagg
     6481 agtccggaaa catcgccatg ataggcaagt gggtgttgtt ccaggtctgc cgcgacatcc
     6541 ggcgcttcgg ggacgcgctc ggcgagcgct atatctccgt caacctgacc accgccgagt
     6601 tctcccgccc gaccctcgtc gacgagatcc gcgagctgct cgagaccgag cgtatccgac
     6661 cggcctcgct caagctcgag ataaccgaga gccagagcat gaaggacgtc gatcaggtca
     6721 tacgcaagat cgaggacctg tccgacatcg ggatagacgt gctgatcgac gacttcggga
     6781 ccggctactc gtcgctcgcc tacctcaagc gcctgccggc caagaccatc aaggttgaca
     6841 aggccttcgt cgaccacata gcggaaagcg atgaggagct ggagttcatc cgcggcgtca
     6901 tgaccatgat cgagagcaag aagaagaacg tgctcgtgga aggcgtgacg tcgctgaacc
     6961 agtacaagct actccgcggg ctcggggccg cgtacctgca gggctactac ttcagcgagc
     7021 cacggtcggg cgacgacttc ctcgccctgc tcaaggccgg gaagccgcta cccgtgtcgg
     7081 cgtgacgatg ggcgcgggac cctgacgagc ccgcgccgca tcttcctaca tgttcttcca
     7141 gaacttgtag tacttgcgct cggcgtcctc gagggacatg agcgggtaaa acttggtctg
     7201 ggtagcggac gcgaacatga agccgtggcg caggaatttg tacgagatgc ggtcgaccgg
     7261 gcagtcgagg gacagtccct tggtattctt acccggcttg gcccgcacct cgaagatcgc
     7321 ctccctgagc ctggcggggt ggatgcccgg cacgaagcag cggataaagg ggttttcctg
     7381 gaattccttg aggaactcgt cgacggtgaa ctcgagctgg gtatagaaca tcaccggcgc
     7441 gcccttgggc atgtccgggt cggtgatgaa tttggcgtac tccaggaagt tgtacttggt
     7501 cagtaccatc atcttgatcg gattgatctc gaggacgatg cgcatctcgt cgcctcgcat
     7561 cttggggtcg taggcggccg actctagctc gacgtagtcg ccgagcgagt tgcagtagta
     7621 cagctttccg aaggcgttga agtccatgtg ctcgaaggtg cggtagctcg agatgaactt
     7681 ggtcgccttc gggcggccgt cctcgtgggg cttgagctcc gcgaaagccg cgtcgatatt
     7741 gaagtacgga tgcctgaagg acgggtcgat ctcgacgaag atcaatcgcc cctcgaagta
     7801 ccgggtcgag ccctgcgtat agtgcttggc gaaacgctcc ggatccagct ggctggcaat
     7861 cagcgcgtgg ttcgggtgca tgatcagata caggtgcttc tcgtcgttca tctcgctacc
     7921 cccaatatga cttgagcatc ggcgcgatca ccagggagat gatcgccatc agcttgatca
     7981 ggatgttgag cgcgggcccg gccgtgtcct tgaacgggtc gccgacggtg tcgccgacga
     8041 ccgccgcctt gtgggcgtcg gagcccttgc cgtgcgcgcg gctctcaatg agcttcttgg
     8101 cgttgtccca ggctccgccc gagttcgaca tgaagatggc caggagcacg ccggtcaccg
     8161 tgacgccggc cagcaggccg ccgagcatct cgatgccccc cgcgaagcct accacgaccg
     8221 ggctggccgc ggcaatgacg ccgggcagta tcatcctgcg cagcgaggcc ttggtcgata
     8281 tgtcgacgca ggcgcggtag tcgggggtct cggtctcctc gagtatgccc ggccgctcgc
     8341 ggaattgcct gcgcacctcc tctatcatcg cgaaggcgga ggcgccgatc gcgtccatcg
     8401 acagcgccga gaacaggaag gggatcatgc cgccgaggag caggccgacg agcaccttgg
     8461 gagcggtgac gtcgaacacg agcgcgttgg agccgccggc ggactgcgcg aaggcgctga
     8521 acaggatgat ggccgtcagc gcggccgagc cgatcgcgaa gcctttgccc accgcggccg
     8581 tcgtgttgcc gacggcgtcg agctcgtcgg tcacgtcgcg tacgtgggga tccatcgccg
     8641 acatgacggc caggcccccg gcgttgtcgg cgatcgggcc gtaggcgtcg acggacagct
     8701 ggatgccgag cgtgacgagc atgccaaggg ccgccatgcc gatgccgtag agcccggcga
     8761 tggagtacga acccagcatg gcgccgcaga tcacgaggac gggcggcagg gtcgacagca
     8821 tgccgacgcc gaggccggta atgatcgcgg tggcggcgcc ggtatcgcat gatcgctcga
     8881 ttttcctgac cggcgccgtg ttggttccgg tgaagaactc ggtcaggagg cctatcgccg
     8941 tgccagagac gaggccgaat accgccgcga gggccacgct gagcgcgttc ctgcctccat
     9001 cgaaggtacc ggaaccgagc gctaggcgcg ccagcgggta cataagcgcg gcggccaggg
     9061 cggccgctcc gaagctaccc gcgttcaagg atttctgggg cgacctgccc ggcttgccgc
     9121 gtacgaggaa ggtacccgct atcgaggcga ggatgcccat cgcggccatc aggatgggca
     9181 ggagagccag gcgtagcctc atgtcgtcgg aggctgccac gccgatgccg agcaccacgc
     9241 aaccgacgat ggagccgacg tacgactcga acaggtcggc gcccatgcca gccacgtcgc
     9301 cgacgttgtc gccgacatta tcggctatga ccgcgggatt gcgcgggtcg tcttcgggga
     9361 tgccagcctc caccttgccg accaggtcgg cgccgacgtc ggccgccttc gtgaagatgc
     9421 cgccgccgac acgcgcgaac agcgctatcg tcgaggcgcc taggctgaag ccggacagga
     9481 tcgggaagat cgtgtcgcgg agcgccccaa tggaggcgcc gaagagggcg acgccgaggc
     9541 cgacgacggc cgcgatgccg agtagggcca gtccgaccac ggtcatgccc atcaccgagc
     9601 ccccggcgaa cgccacgcgc agggccgggc cgatgccgtc cgaggcggcc gaggccgtcc
     9661 tcgagttggc ggccgtcgcg gtacgcatgc cgaagtatcc ggctgccgcc gagcataggg
     9721 cgccaagcac gaaggccagg gcctcgagcc tgagcgagcc ggagttggcc acggccagga
     9781 agagcgcgac cagggcgacg aagggcacga gtaccgtgta ctcgcgccgc aggaaggcct
     9841 cggcgccctc ggctatgtat ccggctatcc gctcgagcgt cgcgtcgccg acgcttcgtt
     9901 tccgtatcca gcgactcttg gctacggcgt agacgatggc tatcgtcccc ccgatcgccg
     9961 cggccagtag caccgcaatg atgttcatcc tgatctacct ccagcacggc gccgtgcgcc
    10021 gcagaacgcg acgcgagcag tataaagtca aaaatcggcc ccgacaatga tttcatgcga
    10081 tgggaccacg aagcttcgta ccaccggggg cttgccagaa cgggcttgag cgagtacctt
    10141 ttgcgaatcg ttcgcactag tcagtttcga tacggagtat gccatgcgcc attcagcaca
    10201 tactggagcg ctcgttcgtg gcgcggtcac cgcggcctcg acgctgatcg tggcgctcgt
    10261 ggtcggttgc ggggcggagc gacccgacga cgggacgctt cccgtcgtcg tggtcagcat
    10321 cgagccccag gcctacttcg tcgagcgcat agccgagggg cgggtatccg tatcggtcct
    10381 cgtcggcccg ggccagagcc cccattcgta cgagcctacg cccaggcaga tggccgacct
    10441 gtcggccgcg tccatgtggc tatgcgtcgg ggccgagttc gagaagagtc tcaggccgaa
    10501 ggccgccgcc ttgtacaaga gcctgcccat cgtcgacacg acgacgggcg tccagtaccg
    10561 caagctcgag gcccatgccc acgacgacgg tgatggcgac catgacgaag aaggcgggct
    10621 cgacccccac gtgtggctag gccgccaggc cgtcaaggcg atggcggcga acatccgcga
    10681 cgcgctcgcg gcggccgatc ccgccggcga ggacgcctac gccgcgaatc acgacgcctt
    10741 cgtgcgcgac gccgacgcgg catacgacga gctggcctcg gctctcgcgg gcctccgggg
    10801 aaccaccgtc ttcgtgtacc acccggcctt cggctacctg ctcgacgagt tcggcatagc
    10861 ccaggaagcc gtggagaccg gtggcaagga gcctacccag aaggccctcg ccgcgatgat
    10921 caaggcggct cgcgaggacg gcgccagcct gatcttcgtg caggcccagt tcccgacgag
    10981 cgccgccgag gccgtagccg aagcgatagg cgccacggtc gtgcccatcg acccgctcgc
    11041 gcgcgactgg ctcgacaacc tgcggcgcat cggcgacgag ctcgccagga tgggcccatg
    11101 actatggagc gttcggttgc tatccgcttc gagtcggtat ccttctccta tccggccgct
    11161 cgggtactcg aggctgccag tttccatgta cacgccgggg agttcgccgc tctggtcgga
    11221 ccgaacggcg cgggcaagac gacggcgctc aggctgatac tcggtctcgt cagacccgag
    11281 tcgggccgcg tgaccgtgct cgggcgctcg cctgaggagg ccaggaacgc gataggctac
    11341 gtgccccagc acgcctccta cgacccggcc ttcccgattt cggtctccga agtggtgcgc
    11401 atgggacgct tgaggtcatc gacgaggcgc tacggccccg aggacgggcg ggccgtcgag
    11461 cgagcgctag cgctggccga cgtcgaggct ctggcgagcc gtccctaccc cgccctctcg
    11521 ggcggccaac gccgcagggt gctcgtcgcg cgggcgctcg ccgccgagcc ggaactgctg
    11581 atactcgacg agccgacggc caacatggac tccgagagcg agggccggct cttcgagacc
    11641 ctcggcgggc tcaagggcca ggcgacgata ctgatagtca cccacgatac cggcttcgtc
    11701 acgtcgctga ccgacgccgt cttctgcgtc ggcgagggag gctccggcgg ccgcatagtc
    11761 aagcaccgcg ccgtgccggc cgcccacgcc gcgagcgtcc agtacggcgg gagcgccttg
    11821 caggtcctcc acgacacggc gctaccggat aacggctgct gctcggacga ccgcgtcggc
    11881 caccctgaaa ggaacgcgcg atgaacggct atatccaggc cctcctcgac ccgacgatgc
    11941 ctttcgtccg caacgccctc gcggccgggc tgttgtcggc ggtactgttc ggcgtgctcg
    12001 gcgcggtcgt caccgtgaaa cgcatagcgg gcctcgccgg agccatagcg cacgcggtgc
    12061 tcggcggcat aggcctgtcg ctgttcctgg cgcccaagat cctgcccgac gggacgcgca
    12121 acgacttccg c
//
LOCUS       DAOPHV010000012        11602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_6662_length_11602_cov_4.594873, whole genome shotgun sequence.
ACCESSION   DAOPHV010000012 DAOPHV010000000
VERSION     DAOPHV010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 11602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11602
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6662_length_11602_cov_4.594873"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..589)
                     /locus_tag="PLQ29_00700"
     CDS             complement(53..589)
                     /locus_tag="PLQ29_00700"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG85189.1"
                     /translation="MTGDVAIRALRADEAEPFLALRKAADAQSRYLLYGAGERRTTVE
                     EQARAIAELLGSGNSTILVAEAEGRDGAGSLVGYLIAKGGGAERNRHAAEIVVAILDA
                     YTGKGIGARLFGELDEWARGAGVTRLGLGLMDGNVRARALYEKMGFREEGRRKAAFLL
                     EGGFVDEILMYKLPSSIL"
     gene            complement(586..1752)
                     /locus_tag="PLQ29_00705"
     CDS             complement(586..1752)
                     /locus_tag="PLQ29_00705"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPG85190.1"
                     /translation="MNVNALPRLTIANLLAGELDRGVAEGVFRCGVVTLTDGGETVMA
                     ACGQARRILDPSESWDAVAADNRSVFDLASLTKIVATLPVSLLTIQSGRMALDDPASR
                     WLPELAAGAGGSWIGAITIRSLLGHFSGLPAWRPYFVRCRDRAAYLSAIADEAASYAP
                     GTTVEYSDLGFMLLAWILERVWDEELPALVDRLVLSPLGMGLTGYPAAARLRFADAAF
                     VPTEAGNEYERSMTLAYAEGRPVVGGPAGRYRIGVAELDGVPWRRGVILGEVHDGNCH
                     YGLGGVSGHAGLFSTAEDLVRFMAFWGPSGPLGPALRAEAFRRQTPDGASPRGLGWIL
                     DGPAATHTGFTGTMLRYRPGDGRALVALTNRVYPSVKDGIGAWRQALVATLEAV"
     gene            1918..3216
                     /locus_tag="PLQ29_00710"
     CDS             1918..3216
                     /locus_tag="PLQ29_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85191.1"
                     /translation="MKRIFVAAAALCALCGLASAQDADSADDYVLQKAREEYAASSGS
                     GDQAATDQDAVMEQSTVIAVPQAPATKTVTTDGDGQSTVITIHIAEDKPSAETDDDER
                     RRRYTPAVIGFVPGLSLPFGVYDTSLSLASVGALTGDVYGFQGSGVFNIADDVGIVQA
                     AGVFNIADSVAGFQGAGVFNIADSIAGFQSAGMFNIADSANGVQAAGLFNIADEIRGV
                     QVAGVVNVADRASGLMIGLVNIADELDGVAIGLVNIIGNGIHDLSVDYQFSTGMAYAT
                     YRSGTPFIYAAFSAGQPATEFMRTPIGLSTGVALGHRFRILFLTADVELGVETPIETA
                     DLALAMRALSNADPSRPETLLPLASAFSPFGSLRASFGFGNRKGFGPYAGIKADFAPS
                     ASGAVPTALRSAFGSAEPYIVPAFGVDLLVWPKWFIGIKF"
     gene            3321..4451
                     /locus_tag="PLQ29_00715"
     CDS             3321..4451
                     /locus_tag="PLQ29_00715"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPG85192.1"
                     /translation="MKRIAIIALALLAVSQTFAQKAAPFGSIDYVEGSAIVVRSGKSL
                     GEANIGDDVLPDDMIKTAADGLVVIALDRSTGMRGTLTIKSKSVAYIRLTPDAAGAKS
                     TIEMVAGQLSSKLAKLSGNPTLQVQTDSVVMGVRGTQFSIAGSVNGSVLVVCTDGAVA
                     CTDGGEPVSVPAGKAVEKKAGERLKLVPVAISSAEQFEKRWIADEIEAFKGNAVRALA
                     DYEKRYTDYMARFNEAFAPLQRSEVLSKWIREDAAGVVPSAGDATTLREKKAIAGDIL
                     ATRKVLFIFERLYYRIVELDQYVMGTTLEKAELRAGLTVGDFVRRVRSDAPALEKRVF
                     LFRYAEKLYEQRNSGGAGLPGMSAPGAGSEDDFFGSSDDWDF"
     gene            4498..5697
                     /locus_tag="PLQ29_00720"
     CDS             4498..5697
                     /locus_tag="PLQ29_00720"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPG85193.1"
                     /translation="MTDYGTPFNYYGPTRLVFGRGVTAGAGALVAGLGVRSVLLVCGS
                     GATRRSAGFAALAASLEAAGVRATVFDKVTPDPHAALVAEAADAARSGSLEAVVAYGG
                     GSPIDCAKSAALAAANGGPILDYVRGKATPSRPALPIVAVTTTAGTGSEMSSAAVTTD
                     LASGQKLGYTNESFFPRIAIVDPENHTSMPTGVTAATGMDALTHAVESYLSLAANPLS
                     DAVNLRVVAMIARSLTRAYRDGADLEARSGMAIASAMAGVAFSQTGLGMVHGFAHPVG
                     ARHGIAHGQANAVILPYVMAALARDAAPRMRDLAAAMGACPSDADPRSGAASLVQAIV
                     DLKKGLGVPEGLSGVGISASDGPTLLTDAIGYRSRPRSPRAFSDAELGRLLDSAIAGD
                     LEAAAAL"
     gene            complement(5710..8751)
                     /locus_tag="PLQ29_00725"
     CDS             complement(5710..8751)
                     /locus_tag="PLQ29_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG85194.1"
                     /translation="MRLRKLTIANIGPYAGLAELDATALGDVFLICGKTGSGKSTIFE
                     AVSYALYGQAAVRRELVSHYAEPEDEAFVELEFSVGPERWRVRRKPARTVPKKRGSGL
                     TDRPAEAVLWRRGLSGWEPRSDRIDDIDATLARVIGLSSKEFTKIVLLPQGEFQRFLE
                     MRTGERTEILEKLFPVDLHDAVSELAKLKAKDAEAKARVLDDRAATMEAALGDDPGAS
                     LAEARSALAEARVADGAALAARDAANAASQLAAVAARAWDEYDAALAERAALEATEAE
                     AASLMARLSRAEAGLGAEAAVKAAGRARAEKAAAESSLAGAEATLAEADAETEGAEKA
                     GLLVAELAEALEAMDREAGLLSARAAAWQRLSAARERLESATAALGAAMAAKSTAETA
                     AAAASATLAAIESGAADAEALVAERSAAAIALESAREAERLAAEAAGFEAETRALEAR
                     TRAAAERSASAETVLASAVDTLAEAEAAVEALRDAQAAFRLASRLGPGSPCPVCGSVE
                     HPAPARPGTMAGGGRGGSSTDDGSGEANALRLEAARAALEAARAEAADAKAALSTLNE
                     AIVARAEGAARYAGALGPEAAEEGLRAARARLVAADRAQAAEAARGRAASAARVEYER
                     LRKILDVAAASVSAAESERSAAAAAAAEASSGSGGDDPGPLLSSLRLRREAAASERAK
                     LESDIELWRRRRGEAVARASEAAARLERAVEALADTEAAVAIALAEAGFETEEAWAAA
                     CLPPPELADARERIRARAAAASSSEARFKAAERSVSGACRPDLAAAEAGRSAADEAYA
                     AARARLDAAAAAERELAAGLDALSAVRAERQAFRERGDRLVALSALLNGNAVGRHVSF
                     KIFTLASYFALVVERASARLREMSDGRYDMRVDDDRSAGRGFVGLDLSVLDSFTGVAR
                     PASSLSGGEKFLASISLALGLSDVIVERAGGVALDSIFIDEGFGSLDDETLDRAMEAL
                     DRVRGERVIGIVSHVAELRSRVPARVEVIKTNAGSSLRVVS"
     gene            complement(8738..10039)
                     /locus_tag="PLQ29_00730"
     CDS             complement(8738..10039)
                     /locus_tag="PLQ29_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HPG85195.1"
                     /translation="MRIFHLADLHLGKTLYERDLLEDQRHALSAVLETARAERPACVV
                     LAGDVFDRAVPSAEAVTLLGEFAASLKAADPNLVVAMIPGNHDSAARLAYLSPVLAMA
                     GVHIAADPGDCVRPVVVERGVERLRLWLLPFLTPGAFGSPAEAASPAPTASTAPTNCQ
                     GELFADDSATEPDAVRGDAADVAPAAEPMAEPTLRSQADLFAEAMRRTGAAMAVARAA
                     DGGARSADVLVCHAFASGGEASESERVFLGNAELVDASGFSAFDYVALGHLHRPQAAG
                     ANGRYPGSLLAYSFSEAGSERGFLSVDVSPGGFSAEFRAVRPLRRMACVSGGFDEVLT
                     DPRYDRYAGDYVEATLSDATAVLNPMDALRRRFPFIMSLRQAAFERGAVEADERRPGR
                     PAASLADDFAVFHREMTGSEPDAATAALFAELYEEASREAP"
     gene            10097..10789
                     /locus_tag="PLQ29_00735"
     CDS             10097..10789
                     /locus_tag="PLQ29_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85196.1"
                     /translation="MTNERTPRRAIVVGDGFLGLAEPAAGDEPDGLLELTAWRAAPDG
                     RTRAVAKRALQPAEIVPLGDVPDAALAAFIPALGPFSFADVDYDPFRSIASSLDRALI
                     RPAVPQLALWAAIEGDLFRFAYDAGFTAALETKRGTAEWARFAALNELYRDLVRAILP
                     FGAAPPGWRPWLTGDGRNGAAARASPAGTASGAAARALARALGRGLSSIRPALMKRAG
                     TANCFTLATIGG"
     gene            10829..>11602
                     /locus_tag="PLQ29_00740"
     CDS             10829..>11602
                     /locus_tag="PLQ29_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG85197.1"
                     /translation="MAIISALGRAARTKASGLLYGLGFFYNVLKETGRFFRRAQVGYK
                     VLIMQILFTGYEALAINAIMAIAIGAAINVIGSSLLPQFGQSQLMYSILIIVITRELG
                     PLLTAFVITARSGTAIATQLGTMVVNHEIEAYMAVGLNPISYLVVPRFIGVVVSMLVL
                     TVYFNIFGLLGSFIVVQVVNPTPIGEYLGALMSTLTVADVLSGLTKALVFGVIVSVVS
                     TYQGFAVNRASTEIPVAGIKAVGSSFILIILADVIVTLVQ"
BASE COUNT         1762 a         4225 c         3894 g         1721 t
ORIGIN      
        1 ccatggcgca gagcgccgcg gcgataagaa cgcgtttctt cattccatcc tcctataaaa
       61 tagagcttgg cagcttgtac atgaggatct catccacgaa gccgccctca aggaggaaag
      121 cggctttcct gcggccctcc tcgcgaaatc ccatcttttc gtagagcgcc ctggcccgga
      181 cattgccgtc catcaggccc agcccgagcc tggtcacgcc ggccccgcgg gcccactcgt
      241 cgagctcccc gaacaatcgg gcccctatgc ccttgccggt atacgcgtcc agtatggcca
      301 cgactatctc cgccgcgtgc ctattgcgtt cggcgccgcc gcccttggcg atcaggtagc
      361 cgacgaggct tccggcgccg tcgcggccct cggcctcggc gaccaggatg gtcgagttcc
      421 ccgagccgag cagctccgcg atagccctag cctgctcctc gaccgtggtc ctgcgctctc
      481 cggctccgta gagcaggtag cggctctggg cgtcggcggc cttgcgcagc gccaggaagg
      541 gctcggcctc gtccgccctc agggcccgta tcgcgacatc gccggtcata ccgcctccag
      601 cgtcgcgacg agggcctggc gccaggcgcc tatgccgtcc ttgacggacg gatacacgcg
      661 gttggtcagc gcgacgagcg cgcggccgtc gcccggccga tagcgtagca tggtaccggt
      721 aaagcccgta tgcgtggccg ccggcccgtc gagtatccag ccgagccctc gcggggaggc
      781 gccgtctggc gtctggcgac ggaaggcctc cgccctcagg gccgggccga gcggacctga
      841 ggggccccag aaggccatga agcggaccag gtcctccgcc gtggagaaca ggccggcgtg
      901 cccgctgacg ccgcccaggc cgtaatggca gttgccgtcg tggacctcgc cgaggatgac
      961 gccgcgccgc cagggcacgc cgtccagctc ggcgacgccg attcgatacc taccggccgg
     1021 gccgccgacc accgggcggc cctcggcgta ggccagggtc atcgaccgct cgtactcgtt
     1081 gccggcctcc gtggggacga acgccgcgtc tgcgaagcgc agccgcgctg ccgcaggata
     1141 gcctgtcagg cccatgccga gcgggctcag cacgagccga tcgaccaggg cgggcagctc
     1201 ctcgtcccat acgcgctcga gtatccaagc cagcagcatg aagccgaggt cgctgtactc
     1261 gaccgtcgtt ccgggcgcgt aggacgccgc ctcgtccgcg atggccgaga ggtacgccgc
     1321 ccggtcccgg cagcgcacga aatacggcct ccacgccgga aggcctgaaa aatgcccgag
     1381 cagcgagcgt atggtgatag cgccgatcca tgagccgccg gcgccagcgg ccagctcagg
     1441 caaccagcgc gaggccgggt cgtccagggc catccggccc gactggatcg tcagcaggct
     1501 gacgggcagg gtcgcgacga tcttggtaag ggacgccagg tcgaagacgg agcgattgtc
     1561 cgccgcgacg gcgtcccagg attccgacgg atcgaggatg cgcctggcct gaccgcaggc
     1621 ggccatgacg gtctcgccgc cgtccgtcag cgtgacgacg ccacagcgga acacgccttc
     1681 cgcgacgccg cgatcgagct cgccagcgag taagttagcg atggtaagcc tagggagagc
     1741 gttgacgttc atggtcccgc tgagtatacg acacaatatt tcatcgagca acaattttta
     1801 ttaaaaggaa ttttcgccga gcaataggat cgttgatttc cgtagccttc gcggtatagt
     1861 acatcatatg cgctccgtac ggaggcataa ccgacactcg ccatagtggg gagaaccatg
     1921 aaacgtatct ttgtagccgc ggcggccttg tgcgccctat gcggcctcgc gtccgcccag
     1981 gatgcggatt cagccgatga ctacgtcctc cagaaagccc gcgaagagta tgcggcctcg
     2041 tctggctcag gcgaccaggc ggctaccgac caggacgccg tcatggagca gtccacggtg
     2101 atcgcggtgc ctcaggcccc ggcaaccaaa accgtcacta cggacggcga cggccagtcg
     2161 accgtcataa ccatccatat cgccgaggac aagccctcag ctgaaacgga cgacgacgag
     2221 cgccggcgcc gctatacgcc cgccgtgata ggcttcgtac cgggcctatc gctacccttc
     2281 ggcgtctacg acacctcgct gtcgctggcc tccgtcggcg ccctgaccgg cgatgtatat
     2341 ggcttccagg gctccggcgt gttcaacatc gccgacgacg tcggcatcgt ccaggccgcc
     2401 ggcgtcttca acatcgccga cagcgtagcc ggcttccagg gcgccggcgt cttcaacatc
     2461 gccgacagca tagccggctt ccagagcgcc ggcatgttca acatcgccga cagcgcgaac
     2521 ggcgtccagg ccgcgggctt gttcaatata gccgacgaga tacgcggcgt gcaggtggcc
     2581 ggcgtcgtca acgtcgccga ccgcgcgtcg gggctcatga taggactcgt caacatcgcc
     2641 gacgagctcg acggcgtggc cataggactc gtcaatatca tcggcaacgg catccacgac
     2701 ctgtcggtcg actatcagtt cagcaccggc atggcctatg ccacctaccg ctcgggcacg
     2761 cccttcatct acgcggcgtt ctcggctggc cagccggcca cggagttcat gcgcacgccg
     2821 atcggcctga gcacgggagt cgcgctgggg caccgcttca ggatactgtt cctgacggcc
     2881 gacgtggagc tcggcgtcga gacgcccatc gagacggcgg acctcgccct ggccatgcgc
     2941 gccctgtcga acgccgaccc ctcgcgcccg gagactctgc taccgctcgc ctcggccttc
     3001 tcgccgttcg gctccctgcg cgcctcgttc ggctttggca accgcaaggg cttcggcccc
     3061 tatgccggca tcaaagcgga ctttgccccg tcggcctccg gcgccgtgcc gaccgcgctc
     3121 cgttccgcct tcggctccgc cgagccgtat atcgtgccgg ccttcggggt cgacctcctc
     3181 gtctggccca agtggttcat cgggatcaag ttctagggcg cggccagcgc tcgaaaaggc
     3241 cgcgctcccg cgttatactc tactcgtcgc ctgcgggcgc cagcccgccg cgcgagtcgc
     3301 gttatccaag gagcgtccat atgaagagaa tcgccataat cgcgctggcc ctactggccg
     3361 tttcccagac cttcgcccag aaggcggcgc ccttcgggtc catcgactac gtcgagggta
     3421 gcgctatagt ggtccgctcc ggcaagtcgc tcggcgaggc caacatcggc gacgacgtgc
     3481 tgcccgacga tatgatcaag accgccgccg acggcctcgt tgtcattgcg ctcgaccgta
     3541 gcacgggcat gcgcggcacg ctgacgatca agtccaagtc ggtcgcttac atccgcctga
     3601 cccccgacgc agctggcgcc aagagcacga tagagatggt ggcgggtcag ctgagttcca
     3661 agctggccaa gctgtccggc aacccgaccc tgcaggttca gaccgactcg gtggtgatgg
     3721 gtgtgcgcgg cacccagttc tcgatagccg gctcggtcaa cggctcggtg ctcgtcgtct
     3781 gtaccgacgg cgcggtcgcc tgtaccgacg gcggcgagcc ggtctccgtg cccgccggca
     3841 aggccgtcga gaagaaggcc ggcgagcgcc tcaagctggt accggtcgcg atctcgtcgg
     3901 cggagcagtt cgagaagcgc tggatagccg acgagatcga ggccttcaag ggcaacgccg
     3961 tcagggccct ggccgactac gagaagcgct ataccgatta catggcccgt ttcaatgagg
     4021 ccttcgcgcc gctccagcgc agcgaggtac tgtccaagtg gatacgcgag gacgccgcgg
     4081 gcgtggtccc ctcggcaggc gacgcgacga cgctacgcga gaagaaggcc atagccggcg
     4141 acatcctggc cacgcgcaag gtcctcttca tcttcgagcg gctatactat aggattgtgg
     4201 aactggatca atacgtgatg ggcacgacgc tcgagaaggc ggagctcagg gccggactca
     4261 cggtcgggga cttcgtccgc cgggtgcgtt ccgacgctcc ggccctcgag aagcgcgtat
     4321 tcctgttccg ctacgccgag aagctgtacg agcagcgcaa ctccggcgga gccggactac
     4381 cgggcatgag cgcgccaggc gcgggctcgg aagacgattt cttcggctcg tccgacgact
     4441 gggacttcta ggcggccctt ccccgccggc ccggttgccc gagccggcgg aggcgatatg
     4501 actgactatg gcacaccctt caactactac ggcccaacca ggctggtctt cgggcgcggc
     4561 gtcaccgccg gcgccggcgc cctcgtagcc gggctaggcg tacgctcggt cctgctcgtc
     4621 tgcggctcgg gcgccacgcg ccggtccgca ggcttcgcgg ctctggcggc ctcgctcgag
     4681 gcggccggcg tccgcgcgac cgtgttcgac aaggtgacac cggaccccca cgcggctctg
     4741 gtcgccgagg cggccgacgc cgcgcgctcg ggctcgctcg aggctgtcgt cgcctacggc
     4801 ggcggtagcc cgatagactg cgccaagagc gcggccctgg cggcggccaa cggcggcccg
     4861 atactcgact acgtgcgcgg caaggcgacg ccatccaggc ccgccctgcc catcgtcgcg
     4921 gtgacgacca ccgccggcac cggcagcgaa atgagctcgg cggccgtcac cacggacctg
     4981 gcgagcggcc agaaactcgg ctacaccaac gaatccttct tcccgcgcat cgccatcgtc
     5041 gacccggaga accacacgtc gatgccgacc ggtgtcaccg cggcgaccgg catggacgcc
     5101 ctgacccacg cggtcgagag ctacctgtcg ctggcggcca acccgctcag cgacgccgta
     5161 aacctgcggg tcgtggcgat gatagcccgg tcgctgacga gggcctatcg cgacggggcc
     5221 gacctggagg ctcgatccgg catggcgata gcgagcgcca tggccggcgt cgcgttctcc
     5281 cagacgggcc tgggcatggt ccatggcttc gcgcacccgg tgggcgccag gcacggaata
     5341 gcccacggcc aggccaacgc cgttatcctg ccctacgtga tggccgcgct cgcgcgggac
     5401 gcggcgcccc ggatgcgcga cctggccgcc gcgatgggcg cctgcccgtc cgacgccgac
     5461 ccgcgctcgg gcgccgcgtc gctcgtacag gccatcgtcg acttaaaaaa gggactaggc
     5521 gtgcccgaag gcctgtcggg agtcgggata agcgcttccg acggcccaac cctgctaacc
     5581 gacgcgatcg gctaccggag ccgccccagg agcccgaggg ccttcagcga cgcggagctg
     5641 gggcgcctgc tcgattcagc gatagccggc gacctcgaag cggccgcggc tctctgagcc
     5701 cgccccggct caggagacga cgcgcaggct cgagccggcg ttcgtcttga tcacctcgac
     5761 gcgggcgggc accctggaac gcagctcggc cacatgagag acgataccta tcacccgctc
     5821 gccgcgcacg cggtccagcg cctccatagc gcggtcgagc gtctcgtcgt ctagggaacc
     5881 gaagccctcg tcgatgaaga tcgagtcgag agccacgccg ccggcccgct cgacaatcac
     5941 gtcggacagg ccgagcgcca gcgatatcga cgccaggaac ttctcgccgc ccgacaggct
     6001 cgacgcgggc cgcgccacgc cggtgaagga gtcgagcacc gagaggtcca gcccgacgaa
     6061 gccgcggcct gcggagcggt cgtcgtccac ccgcatgtcg taacggccgt cggacatctc
     6121 gcgcagccgc gccgacgccc gctcgacgac cagcgcgaag tacgaagcca gcgtgaagat
     6181 cttgaacgat acgtggcgcc cgaccgcgtt gccgttgagc agggccgaca gggcgacgag
     6241 gcggtcgccg cgctccctga acgcctgccg ctccgctcgg accgccgaca gcgcgtcaag
     6301 tccggcggcc agctcgcgct cggcggcggc ggcggcgtcc aacctggccc tggcggccgc
     6361 gtaggcctcg tcggcggccg accgcccggc ctccgcagcg gccaggtccg gccggcatgc
     6421 gcccgagacc gaacgctccg ccgccttgaa ccgggcctcg gacgaggacg cggccgccgc
     6481 cctcgcccgt atccgctccc gcgcgtcggc gagttcgggc ggcggcagac aagccgcggc
     6541 ccaggcctcc tcggtctcga agcccgcctc ggcgagcgcg atcgcgacgg ccgcttcggt
     6601 atcggcgagc gcctcgaccg cgcgctcgag cctcgcggcg gcttccgacg cccgcgccac
     6661 ggcctcgccg cggcgccgcc gccacagctc gatgtcggac tcaagcttcg ctcgctccga
     6721 ggccgcggcc tctcgcctga gtcgcaagga cgagaggagc ggccccgggt cgtcgccgcc
     6781 ggagccgctc gaggcctcgg ccgcggcggc cgcggccgcc gatcgctcgg attcggccgc
     6841 cgataccgag gccgccgcca cgtccaggat ctttctgagc cgttcgtact cgacccgcgc
     6901 ggctgaagcg gcgcgcccac gcgccgcctc ggcggcctga gcccggtcgg ccgccacgag
     6961 ccgcgcccgg gctgctcgca ggccctcctc ggccgcctcc ggcccgaggg caccggcgta
     7021 gcgggccgcg ccctccgctc tcgcgactat ggcctcgttc agggtggaca gcgcggcctt
     7081 ggcgtctgcc gcctcggccc tggccgcttc aagcgcggcg cgggccgcct cgagccgcag
     7141 cgcgtttgcc tcgccggaac cgtcgtcggt cgacgaccct cctcttccac cgcccgccat
     7201 cgtaccaggc ctcgctggcg ccggatgttc gaccgagccg cataccggac aaggcgatcc
     7261 aggtccgagg cgggaggcca gcctgaaggc ggcctgcgcg tcgcgtagcg cctcgacggc
     7321 ggcctcggcc tcggccagcg tatcgaccgc ggaggccagg acggtctcgg ccgaggcgga
     7381 acgctcggcc gcagcccgag tccgcgcctc gagcgcgcgg gtctccgcct cgaagcccgc
     7441 ggcctcggcc gccagccgct cggcctcgcg ggccgactcg agcgctatgg cggcggcgct
     7501 acgctcggct accagggcct cggcgtcggc cgccccggac tcgatcgcgg ccagcgtggc
     7561 tgacgcggcg gcggcggcag tctcggcggt cgacttggcg gccatggcgg cgcccagggc
     7621 ggccgtggcc gactcgagcc gttccctggc ggccgataac cgctgccagg cggccgcgcg
     7681 ggccgacagg agtccagcct cgcggtccat cgcctcgagc gcctcggcca gctcggccac
     7741 gagcaggcca gccttctcgg cgccctcagt ctcggcgtcg gcctcggcca gggtagcctc
     7801 ggctccagcg agagacgact cggcggccgc cttctcggcc cgggcgcgcc cggccgcctt
     7861 cacggccgcc tcggcgccga gcccggcttc ggcccgcgac aacctcgcca tcagcgacgc
     7921 ggcctcggct tcagtggcct cgagggcggc ccgctcggcg agcgcggcgt cgtattcgtc
     7981 ccaggccctg gccgcgacgg cggccagctg ggacgcggcg ttggcggcgt cgcgggcggc
     8041 cagggccgcg ccgtccgcca ccctggcttc ggcgagcgcc gagcgcgcct ccgccaacga
     8101 ggccccagga tcgtcgccga gagcggcctc catcgtagcg gcccggtcgt cgagcactct
     8161 ggccttggcc tcggcgtcct tggccttgag cttggccagc tcgctgacgg cgtcgtgcag
     8221 atcgacgggg aacagcttct cgagtatctc ggtccgctcg ccggtcctca tctcgaggaa
     8281 gcgctggaac tccccctgag gcaacagcac gatcttggtg aattccttgg acgacagccc
     8341 gatcacgcga gcgagcgtag cgtcgatatc gtcgatgcgg tcggagcgcg gctcccaacc
     8401 ggacagtccc cggcgccata ggacggcctc ggccggccga tcggtcagcc ccgagccccg
     8461 cttcttaggc accgtcctgg cgggcttgcg tcgcacgcgc catcgctccg ggcccacgga
     8521 gaactccagc tcgacgaagg cttcgtcctc gggctcggcg taatgcgata cgagctcgcg
     8581 ccggacggcg gcctggccgt atagagcgta cgagacggcc tcgaagatgg tgctcttgcc
     8641 cgagccggtc ttgccgcaga tcaggaacac gtcgccgagg gctgtcgcgt ccagctcggc
     8701 caggccggcg taggggccga tattggctat cgtcagctta cggagcctca cggctagcct
     8761 cctcgtatag ctcggcgaag agcgccgccg tcgcggcgtc cggctcgctc ccggtcatct
     8821 cgcggtggaa cacggcgaag tcgtcggcca gcgaagcggc aggccggccc ggccttcgct
     8881 cgtccgcttc gacggcgccc cgttcgaagg cggcttgccg gagcgacatg atgaacggga
     8941 agcgccggcg cagggcgtcc atcgggttga gtaccgccgt cgcgtccgac agggtagcct
     9001 cgacgtagtc gcccgcgtag cggtcgtagc gcgggtcagt caggacctcg tcgaaaccgc
     9061 cggagacgca agccatgcgc ctgagcggcc gcacggccct gaactctgcc gagaagccgc
     9121 ccggcgagac gtcgaccgac aggaagccgc gttccgaccc ggcctccgag aaactatagg
     9181 caagcagcga tcccggatag cggccgttcg ccccggccgc ctgggggcgg tgcaggtggc
     9241 cgagcgccac gtagtcgaag gcggagaagc ccgacgcgtc gaccagctcg gcgttgccga
     9301 ggaagacgcg ctccgactcg ctcgcctcgc ctcccgaggc gaaggcgtgg cagacgagga
     9361 cgtcggccga ccgggcgccg ccgtcggcgg ccctcgccac ggccatcgcg gcgcccgtcc
     9421 ggcgcatcgc ctcggcgaag aggtcggcct gggaacgcag ggtcggttcg gccatcggct
     9481 cggccgccgg cgctacgtcc gcggcgtcgc cccggacggc gtccggctca gtcgcgctat
     9541 cgtcggcgaa cagctcgccc tggcaatttg tcggcgcggt cgacgcggtc ggcgcgggcg
     9601 acgccgcctc ggccggtgaa ccgaaggcgc caggcgtcag gaagggcagg agccagagcc
     9661 gcagccgctc gacgccgcgc tcgacgacga ccgggcggac gcagtcgcca gggtcggcgg
     9721 ctatatggac gccggccatg gcgagcaccg gcgacaggta agcgagcctg gcggccgagt
     9781 cgtgattgcc gggtatcatc gcgacgacca ggttggggtc ggcggccttc aatgacgctg
     9841 cgaattcgcc gagcagggtt accgcctcgg ccgacggtac ggcccggtcg aacacgtcgc
     9901 cggccaggac cacgcaggcc ggacgctcgg ccctagccgt ttcgagcacg gccgacagcg
     9961 cgtggcgctg atcctcgagc aggtcgcgtt cgtacagagt cttgcccagg tgaaggtcgg
    10021 ccaggtggaa gattctcata tgggtcgatc ctaggcgggc cgtgacagat ggtcaagctc
    10081 ggtataggat gggaccatga cgaacgaacg aacgccgcgc cgcgccatcg tcgtcggcga
    10141 tggcttcctg ggactggccg agccggcggc gggcgacgag cccgacggcc tcctcgaact
    10201 gaccgcctgg cgcgccgcgc ccgacggccg gacccgcgcc gtcgccaagc gggcgctcca
    10261 gcccgccgag atcgtcccgt tgggcgacgt cccggacgcc gcgctcgcgg ccttcatccc
    10321 cgcccttggg cctttctcgt tcgccgacgt cgattacgac cctttccgat cgatagcctc
    10381 gtcgctcgac cgcgccctga tccgtcccgc cgtcccgcag ctggcgctct gggccgcgat
    10441 cgagggagac ctgttccggt tcgcgtacga cgccggcttt accgcggctc tcgagacgaa
    10501 acgcggcacg gccgaatggg cccgcttcgc cgctctcaac gagctgtacc gcgacctggt
    10561 gagggccatc ctgccgttcg gcgcggctcc ccccggttgg cggccctggc tgaccggcga
    10621 cggacgtaac ggagcggccg cccgcgcatc gccggccgga accgcaagcg gcgccgcggc
    10681 gcgggccctg gcccgcgccc tcggtagggg cctatcgtcg atccgtcccg ccctgatgaa
    10741 acgggcagga accgcgaact gcttcacgct cgccacgata ggcggttgaa cggaaggccc
    10801 ggcgggccga tactcgaagc gaggtagcat ggcgatcata tcggcgctcg ggcgcgcggc
    10861 ccgtaccaag gcctcgggcc tcctgtacgg gctcggcttc ttctataacg tcctcaagga
    10921 gaccggtcgc ttcttccggc gcgcgcaggt gggctataag gtcctgatca tgcagatcct
    10981 gttcaccggc tacgaggccc tcgcgatcaa cgcgatcatg gccatagcga taggcgccgc
    11041 gatcaacgtg ataggctcga gcctcctgcc gcagttcggc cagagccagc tcatgtactc
    11101 gatcctgatc atcgtgatca cccgcgagct cgggccgctc ctgaccgcct tcgtgatcac
    11161 tgcgcgctcg ggcaccgcca ttgccaccca gctcggcacg atggtcgtca accacgagat
    11221 cgaggcctac atggccgtcg ggctcaaccc gatatcctac ctcgtggtac cgcgcttcat
    11281 cggcgtggtc gtctcgatgc tcgtgctgac cgtctacttc aacatcttcg ggctcctcgg
    11341 ctcgttcatc gtggtgcagg tggtcaaccc gacgccgata ggcgagtacc tcggcgccct
    11401 catgtcgacg ctgacggtgg ccgacgtcct gtccggcctg accaaggccc tggtcttcgg
    11461 cgtgatcgtc tcggtcgtgt caacctacca gggcttcgcg gtcaaccggg ccagcaccga
    11521 aattccggtc gccggcatca aggcggtcgg ctcgagcttc atcctgatca tcctggccga
    11581 cgtgatcgtc accctggtgc ag
//
LOCUS       DAOPHV010000013        11490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_6759_length_11490_cov_4.109314, whole genome shotgun sequence.
ACCESSION   DAOPHV010000013 DAOPHV010000000
VERSION     DAOPHV010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 11490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11490
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6759_length_11490_cov_4.109314"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1560)
                     /locus_tag="PLQ29_00745"
     CDS             complement(148..1560)
                     /locus_tag="PLQ29_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85198.1"
                     /translation="MEGLPFLLAWPTAGRRACLVDSVAGFVANAAEHGRAVSDVLVAS
                     DGAGEGLEAGVAAGLAELEQRYGFSSTLSNQRTRRAEIADLSVDFEPEVLEYALLPCP
                     DGPGWGVNVNAAMLAGAGGLLVSCDDDVFCRPARPATADYSDDPRFSMSTEYQVGELD
                     YHDSRESLLSSMRQVDVDIVGRYRAVLGMSAGELAGEGAWRDDDSPVMVASPGMYGDS
                     AMGGSRTALSLVGAARERLMAGPYERLRLSRELVRVAPRPTLSPGTQLIMTQTGFDDR
                     SPPPPFLPFGRNPDGLCAVLTRLVYPASLTAYLDFGLLHAPPEPRVAVRADLTSYRVN
                     LAELVMALVIANRPAASVADPAERFRRIAAALVDAAAFGNSALVDALHGSWAPQLGRY
                     VERLEVMLDTHDREPAAWAADVGEHIDAVNALLREPSALFGAAGCGLSIDGVRRHLRL
                     YGTLLACWPELHARHAAPEK"
     gene            complement(1563..2516)
                     /locus_tag="PLQ29_00750"
     CDS             complement(1563..2516)
                     /locus_tag="PLQ29_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85199.1"
                     /translation="MATITGPGVDLSTEAVTVSAGSSAELSFPSVPVRSGLILTVRVF
                     DADGAIVGERVVSDLALDGDQSNVTVGVAPYGAERLSLGTLVELGYSEPILSIDSVED
                     RLYIYAVELPTRGLYSAKLSIDYFTHVDDQPFLLYDEDGIEVGDYVDDDTSRSGFGLF
                     TNDASEPALHYMVFQGASSWVGAEISAHRVFESSMAVLGRLSNDPVGVALIDTTNGMD
                     LGYISPIGDGSHAEQSLRLKNISGKELTIAVCTFESGSSDAFSLPTVPATTIPPGGIS
                     EFIVRFEPTSGGAFSATLLIESDDDADIDFSMSFSGSASAG"
     assembly_gap    2975..3074
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3515..4273)
                     /locus_tag="PLQ29_00755"
     CDS             complement(3515..4273)
                     /locus_tag="PLQ29_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HPG85200.1"
                     /translation="MTLRLLRTGVARGAFNMGLDEAVLGSVAEGRQPPTLRFYGWNPP
                     TVSLGYFQGLDDEVDREACRAAGVDVVRRITGGGAVFHDDELTYSVVIPERHPLARAD
                     IMESYRFLCSGLVDGLARLGLRAAFAPLNDILVDGRKVSGNAQTRRKGCILQHGTVLL
                     SVDPERMFSLLKVPSEKLKGKLIQDIKDRVRGLDQLLGRPIGFDEAADALVAGFAAAL
                     GVELSLAEPTPTELSEAERHAEERYANEAWTSKR"
     gene            complement(4263..4541)
                     /locus_tag="PLQ29_00760"
     CDS             complement(4263..4541)
                     /locus_tag="PLQ29_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85201.1"
                     /translation="MTVQRVFKSPGTKLLRLTVDLEAGVVRSLSMRGDFFAHPEEGFD
                     RVEASLAGVPVGAFEATFRSALEREGVSLYGLDPADVARLIEEAAHDA"
     gene            complement(4538..5452)
                     /gene="lipA"
                     /locus_tag="PLQ29_00765"
     CDS             complement(4538..5452)
                     /gene="lipA"
                     /locus_tag="PLQ29_00765"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPG85202.1"
                     /translation="MSFPVTSSSASEPGSARPLRKPEWLKVAIPATEGSRMMTRLVGR
                     RGLHTVCDSARCPNKAECWGCGTATFMILGDTCTRNCRFCAVPTGKHGDEPNADEPRQ
                     LAEAIAELGLRYAVLTSVDRDDLADRGAGHFAACVRAIKVSNPDVRVEVLTPDYREGE
                     IELILAEGPDALDHNVETVPRLQGVRDARASYAASLHTLELAASWSPGRPAVVKSSLL
                     LGLGETRDEVLASMDDLRAIGCDAIVLGQYLRPTGAQVPVVEYVHPDAFAAYAESARE
                     RGFTAVVAGPLARTSYHAREAFGEGGRA"
     gene            complement(5758..7188)
                     /locus_tag="PLQ29_00770"
     CDS             complement(5758..7188)
                     /locus_tag="PLQ29_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPG85203.1"
                     /translation="MNHTLERRPLFGPAAFYKEVLAVALPVMAQQAIQSLVSLIDNFM
                     VAGLGDAKMAGVNVANQVNFVYMVVMMTLSAAGGIYMSQYRGAKDEEGMRQAYRFKAI
                     MTLGVSALYFAVCQVMPSSLVTMMTIGNAAQAEIVAHGASYLRLVSWTFLPMALSQSI
                     GSAFREIGRPNAPLVISVVATGVNTFFNWVMIYGNLGAPALEIRGAAIATIIARVVEL
                     AAFVTYARAVKAPFYVPLAGLLKARWKLFREILGKSSLMLLSETSWVVSETVTTALYN
                     GRGGAEVVAGMSAGWAIANIFFLVFTGIHVSTGVVVGGLLGADKLDEAKVKARWIMSG
                     SVIGGTVVALLQVASIAAVPIVFMNLTPAATKVTAGLLVVIAVYLPLWSLLNSQFAVS
                     RAGGDTMMGVVVDVGVTYALFIPGAFALALLTPIGPVYMYAIVKLTDLVKWVVACRWL
                     KKERWLRNLAAPADGAAEKAVVNGAG"
     gene            complement(7360..8163)
                     /locus_tag="PLQ29_00775"
     CDS             complement(7360..8163)
                     /locus_tag="PLQ29_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85204.1"
                     /translation="MRANDTRPAAAGSRSMATGARLVAAGSRLVAADLRLVGRDPLLC
                     IMPFVPFLAAIALRLALPPLAAFIAKATGFMLLDYADLIRVVLILFPGTFFGMMSGFL
                     LLDDRDDGVSPYWSVSPVGRSGYLAARLALFCTAALVAGLAVGPAFGLGGRSFAADAS
                     VAVVGALQAPFFALFLAAIASDKVEGLATLKALSALDLAPLAVLLPLPARALAWPFPQ
                     YWAARLALGASLGPGTGVAVGSPEALGLGLAVSAAWLSFLYARYRRRVD"
     gene            complement(8165..8866)
                     /locus_tag="PLQ29_00780"
     CDS             complement(8165..8866)
                     /locus_tag="PLQ29_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85205.1"
                     /translation="MNAALFAADLRLLWRHGFAIAYLVVAVLYAAALSALPRAWADAV
                     LPVLAWSDPTFFCFFFAGASVCLDLAQGTFRSLFASPLRPSAYLIVKALDLGILSFAM
                     AALVSASSRGAGFRLWPLAVSAVAGGAPTALLGATLALRLKSVNRFMMGSIPAFLVLA
                     LPAVQYAAGRYLPPWFGLVADLSPGDGSLAFARAAYTPVPAGELALGALAAAAWTVAI
                     ARFALAPAVNAARGD"
     gene            complement(8863..9786)
                     /locus_tag="PLQ29_00785"
     CDS             complement(8863..9786)
                     /locus_tag="PLQ29_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG85206.1"
                     /translation="MIRVRDLRFTYPKGTSEAIRGIDFAVAPGEVFGFLGPSGAGKST
                     TQKILLGSLRGYSGSATVGSVEASGADSRYRAGIGAAFEFPCFYQKLTGLENVRLFAS
                     FYPGPKVDPRPLFERLGLADAMDWRLAEYSKGMRMRLNLIRAVIHRPRVLFLDEPTSG
                     LDPANARVVRDIVRERAADGAAVFLTTHDMAAADATCDRVAFIVDGRIAACDEPAALK
                     RRYGKRAVVVDREPGPEVGAAAGGSAAGASDGASDGASEKTAQAEYPLDGLADDAAFI
                     AELRKPGLLGVRTLDSSLDGIFLRLTGKELV"
     gene            complement(9783..10508)
                     /locus_tag="PLQ29_00790"
     CDS             complement(9783..10508)
                     /locus_tag="PLQ29_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPG85207.1"
                     /translation="MPTAFDDRERAAIRERLLAAAVDALGRGGLGAASVAELAAAASI
                     AKGSFYLFYDSKEELFMDALEGIERDYRAIFEAAPALPGTAFERVHAAFQAAFAKVTS
                     DPAVTSMDGRTLERLTRALPPERVARHVEADAAAMATIAAAWSAAGLLGPGVGAAELA
                     GAGYAVFLMATGLRNLPEGPRAATVAVVALGLALALAGTEGAIGEGSDERGGRRDSDT
                     LRRDSDTLRRYSDTRRHEERRTR"
     gene            10676..11479
                     /locus_tag="PLQ29_00795"
     CDS             10676..11479
                     /locus_tag="PLQ29_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPG85208.1"
                     /translation="MSFFKPFALERFFAKHEFSAKYLLCSSDCESFPVRELLAMDEGS
                     IDRLLDLRLGYTETRGAPSLREAIAGHYAGLGPEGVFVHSGAEEAILNLYLAALKAGD
                     RVVVNYPCYQSLAELPRSLGCEVIPWELRQVDDGRGKSRWRLDPDELPALSGGKATMI
                     VLNAPHNPTGALPTRDEFEAIVSYARKTGAILFVDEVYRRLERDRAARLPSVCEAYEN
                     GVALDVLSKHSGLAGLRIGWLASRRVDLLDAVAVVKDYNSICSSAPRSR"
BASE COUNT         1887 a         3853 c         3977 g         1673 t
ORIGIN      
        1 cgccgactgg cgcggcgatc gctagccgtc gtttaacctg actcgctgcg ctcgcgggtt
       61 aacgacggca tcgctacgct cgcaggttaa cgacggcatc gctacgctcg cgggttaacg
      121 gcggcgcgtg tggggcggcg tgtcgtgtta cttctcaggg gcggcatgtc gcgcgtgcaa
      181 ctcgggccag caagcgagca gcgttccgta caggcgtagg tggcggcgga cgccgtctat
      241 ggataggccg caaccggccg cgccgaagag ggcgctcggt tcgcggagca gggcgttgac
      301 cgcgtcgata tgctcgccga cgtcggcggc ccacgcggcc ggctcgcggt cgtgggtatc
      361 gagcatgacc tcgagacgct cgacgtagcg gccgagctgg ggcgcccagg atccgtgcag
      421 ggcgtccacg agggcggagt taccaaaggc ggccgcgtcg acgagggccg cggcgatacg
      481 cctgaagcgc tcggccgggt cggcgaccga ggccgccggg cgattggcta tcacgagcgc
      541 catgacgagc tcggccaggt tgacgcgata cgacgtcagg tcggcgcgga cggcgacgcg
      601 cggctcgggc ggcgcgtgga ggaggccgaa gtcaaggtag gccgtcaggc tggccggata
      661 gacgaggcgc gtcagcacgg cgcagaggcc gtcggggttg cgtccgaagg gcaggaaggg
      721 cggcggcggc gagcggtcgt cgaagcccgt ctgcgtcatg atcagctggg taccgggcga
      781 cagggtcggg cgcggcgcta cgcggactag ctcgcgcgac aagcgtagcc gctcgtaggg
      841 gccggccatc aggcgctccc tggccgcccc gacgagcgac agcgcggtgc gggagccgcc
      901 catcgccgag tcgccgtaca tgcccgggct cgctaccatg accggcgagt cgtcgtcccg
      961 ccacgcgccc tcgcccgcca gctcaccggc gctcatcccc aataccgccc ggtatcggcc
     1021 gacgatgtcc acgtcgacct ggcgcatcga cgacaggagg gattcacggc tatcgtggta
     1081 atctagctcg ccgacctgat actcggtaga catggagaag cgcgggtcgt cggagtagtc
     1141 cgccgtggcc ggtcgggccg gccggcagaa cacgtcgtcg tcgcacgaca cgaggagccc
     1201 tccggcgcct gcgagcattg ccgcgttcac gttgacgccc cagccggggc cgtcagggca
     1261 tggcaggagg gcgtactcga gcacctcggg ttcgaagtcg acagacaaat ccgctatctc
     1321 ggcgcgcctc gtcctctggt tcgacagggt cgacgagaaa ccgtagcgct gctccagctc
     1381 ggccagtccc gcggccacgc cggcctcgag cccctcgccc gccccgtccg aggccacgag
     1441 cacgtccgac acggcccgcc cgtgctccgc tgcgttggcc acgaagccgg cgacactatc
     1501 gacgaggcag gccctgcgcc cggccgtagg ccaggcgagg aggaagggaa gtccttccac
     1561 cgctagccag ccgaagcgga tccgctaaag ctcatcgaaa aatcgatatc agcatcatca
     1621 tcgctttcga tgagcaaggt agcgctaaat gcaccaccag aagtcggttc gaaccggaca
     1681 ataaattcac taataccgcc aggcggtata gtcgtcgcgg gtacagtggg cagggagaac
     1741 gcatcgctac taccgctctc gaacgtgcac acggctatgg ttagttcctt gccgctgatg
     1801 tttttcaggc gtagcgattg ctcggcatgc gaaccgtcgc ctatcggcga tatgtatcct
     1861 aggtccattc cattagtggt atcgatcaac gcgacgccaa cggggtcgtt gctgagacgc
     1921 ccaaggacag ccatagaaga ctcgaatact cggtgagcgg agatctccgc gccgacccat
     1981 gatgaggctc cctggaatac catgtaatgg agcgcgggct cggaggcgtc gttcgtgaac
     2041 aatccaaaac cggacctcga ggtatcgtcg tcgacgtagt ctccgacctc gataccgtct
     2101 tcgtcatata ggaggaatgg ctggtcgtcg acatgtgtga agtaatcgat gctcagttta
     2161 gccgaataaa gtccgcgcgt cggtagctct acggcgtaga tatagagccg atcctcgaca
     2221 gaatcgatcg aaagaatagg ctcgctatac cctagctcaa cgagagtccc caaggacagt
     2281 cgctccgccc cgtacggcgc gacgccgacg gttacattgc tctggtcgcc gtcgagggcg
     2341 aggtcgctca ctacccgctc gcctacgatg gcgccgtccg cgtcgaagac ccgtacggtc
     2401 aggatcagtc cggagcgcac gggcacggac gggaagctaa gctcggcgct cgagcctgcg
     2461 ctcaccgtca ccgcctcggt cgagaggtcg acgcccgggc cggtgatagt cgccacggcg
     2521 tacgaggcgt caggatggat gaaacgcggc gaggccgcct cgtcggcgtc caacagttcg
     2581 tcggctatag ccttgcggac ggccgccggc acgtcgctag ataggtcggc cggtacctcg
     2641 atgtttatgc gcagtggttt atagccggaa acggaaacgg gggctcggca ggcggataag
     2701 caggccacgg ccagcgcggt tgacagcgtg attgaaataa gacgtttcat gaatcataac
     2761 tcctatggtc gagtactaaa cgaacgccag tagcggatgg aaccgtgttc gatactaacg
     2821 ataactatac cacgcatgat gagtaaatac cacgatcgcc gactggcgcg gcgatcgtta
     2881 gccgtcgttc aacctgactc gctgcgctcg cgggttaacg gcggcgcgtg cggggcggcg
     2941 cgtgcggggc ggcgcgtgcg gggcggcgcg tgcgnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnncgtgcg gggcggcgcg tgcggggcgg cgcgtgcggg gcggcgcgtg
     3121 cggggcggca tcgctgcgct cgccgtatcg gggggatggc gactgatacc ctacccgcta
     3181 accgccacga cacgccgccc ctttgaacgt tgcgatcgcc gactggcgcg gcgatcgccg
     3241 gccgtcgttt aacctgactc gctgcgctcg caggttaacg gcggctgctc actacgaacg
     3301 gaacatctct ggctgatacc atacccgcta acgttcacga cacgcagctc ctgtgaacgt
     3361 tgctcgtaca caggcctcgc atgcacgcgg aggcggatgt ccaggagacg gcggccctcg
     3421 cgtagcgagg gtcccgcctc ctggacaggc cccgccgaga tgctatacga ggcctgtagt
     3481 aattcccgag cgtcaggggc ggcgtgtcgt atcgctatcg cttcgacgtc cacgcttcgt
     3541 tggcgtaccg ctcctcggcg tggcgctcgg cctcggacag ctcggtgggg gtcggctcgg
     3601 ctagggacag ctcgacgccg agggcggcgg cgaagccggc gacgagggcg tcggccgcct
     3661 catcgaaccc tatcgggcgg cccaggagct ggtcgagccc gcggacgcgg tccttgatgt
     3721 cctggatgag cttgcccttg agcttctcgg aggggacctt gagcaggctg aacatgcgct
     3781 cggggtcgac gctgagcagt acggtaccgt gctgcagtat acagcccttc ctgcgggtct
     3841 gggcgttgcc cgagaccttg cggccgtcga cgaggatgtc gttgagcggc gcgaacgcgg
     3901 cgcggaggcc gaggcgggcg aggccgtcga cgaggccgga gcacaggaag cggtacgact
     3961 ccatgatgtc ggccctggcc agcgggtggc gctcggggat gacgacgctg taggtcagct
     4021 cgtcgtcgtg gaagacggcg ccgccgccgg ttatcctgcg gaccacatcg acgccggcgg
     4081 cccggcacgc ctcgcggtcg acctcgtcgt cgagcccctg gaagtagccg agcgacacgg
     4141 tcggcgggtt ccagccgtag aagcgcagcg tcggcggctg ccgaccctcg gccaccgagc
     4201 cgagcacggc ctcgtcgagg cccatgttga aggcgccgcg cgcgacgccc gtcctgagca
     4261 gcctaagcgt catgggcggc ctcctctatc aacctggcga cgtcggccgg gtcgaggccg
     4321 tacaggctga ctccctcgcg ctcgagggcc gagcggaagg tcgcctcgaa cgcgccgacc
     4381 gggacgccgg ccagcgacgc ctcaacgcgg tcgaagcctt cctccggatg ggcgaagaag
     4441 tcgccgcgca tcgacaggct ccgtacgacg ccggcctcga ggtcgacggt cagcctcagg
     4501 agcttggtgc caggcgactt gaatacgcgc tggacggtca cgcgcgtcct ccctcgccga
     4561 aggcctcgcg ggcgtggtag ctcgtacgcg cgagcggtcc cgcgacgacg gccgtaaacc
     4621 cgcgctcgcg ggcgctctcg gcgtaggcgg cgaaggcgtc ggggtggacg tactcgacga
     4681 cggggacctg ggcgcccgtc ggccgcaggt actgtccgag cacgatggcg tcgcagccga
     4741 tcgcgcgcag gtcgtccatc gaggcgagca cctcgtcgcg ggtctcgccg aggcccagga
     4801 gcaggctgga cttgacgacg gccggccggc ccggggacca cgaggccgcc agctcgaggg
     4861 tatggagcga cgcggcgtag ctcgccctcg cgtcgcgcac gccctgcagg cgaggcacgg
     4921 tctcgacgtt gtggtcgagc gcgtccggcc cctcggccag tatgagctct atctcgccct
     4981 cgcggtagtc gggcgtcagc acctcgacgc ggacatcggg attggacacc ttgatcgcgc
     5041 gcacgcaggc cgcgaaatgc ccagcgccgc ggtccgcgag gtcgtcccgg tcgacgctcg
     5101 tcagcacggc gtagcgtagg ccgagctcgg ctatggcctc cgcgagctgg cgcggctcgt
     5161 cggcgttcgg ttcgtcgcca tgcttgcccg tgggcacggc gcagaagcgg cagttcctgg
     5221 tgcaggtatc gccgagtatc atgaaggtgg cggtgccgca gccccagcac tcggccttgt
     5281 tcgggcagcg ggcggaatcg cagacggtat ggagcccgcg gcggccgacg agccgcgtca
     5341 tcatgcgcga cccctcggta gcgggtatgg ctaccttgag ccactcgggc ttgcggagcg
     5401 gccgggccga accgggctcc gaggcggaac tactcgttac gggaaatgac aaggctacga
     5461 ccctcccggg gccgcgcgcg gcgcccccat cggcagccat cgtagcgtaa aagccgcggg
     5521 ggcgtctatt cgagctgtgg tacgaagcct atgtatgccc gccgctcagc cggcgccgtt
     5581 cacgacggtc ttctccgccg ctcagccggc gccgttcacg acggtcttct ccgccgctcg
     5641 gccggcggct cggccgacgg ctcagccggc gccgttcacg acggccttcc ccgccactcg
     5701 gccggcggct cggccgacgg ctcagccggc gccgttcacg acggccttct ccgccgctca
     5761 gcctgcgccg ttcacgacgg ctttctccgc cgctccgtcc gccggcgccg cgaggttcct
     5821 gagccagcgc tctttcttga gccagcggca tgccacgacc cacttgacca ggtcggtcag
     5881 cttgacgatc gcgtacatgt acacgggccc tatcggcgtc agcagggcca gcgcgaaggc
     5941 gcccgggatg aacagcgcgt aggtcacgcc gacgtcgacg accacgccca tcatcgtatc
     6001 gccgccggcc cgcgacacgg cgaactgaga gttgaggagg ctccagagcg gcaggtagac
     6061 cgcgatgacg acgaggaggc cggcggtcac cttggtcgcg gcgggcgtca ggttcatgaa
     6121 cacgatcggc acggcggcga tgctcgccac ctgcaggagc gcgacgaccg tgccgccgat
     6181 taccgagccg ctcatgatcc agcgcgcctt gaccttggcc tcgtccagct tgtcggcgcc
     6241 caggaggccg ccgacgacga cgccggtcga cacgtggatg ccggtgaaca ccaggaagaa
     6301 gatattcgct atcgcccagc cggccgacat gccggccacc acctcggcgc cgccacggcc
     6361 gttgtacagg gccgtcgtca cggtctccga taccacccag ctcgtctcgg acaggagcat
     6421 caggctggac ttgcccagga tctcgcggaa cagcttccag cgcgccttca agaggcccgc
     6481 gagcggtacg tagaacggcg ccttgaccgc tcgggcatag gtcacgaagg ccgccagctc
     6541 gacgacgcgc gcgatgatgg tcgcgatggc ggcgccgcgt atctccaagg ccggagcgcc
     6601 caggttgccg tagatcatca cccagttgaa gaaggtattg acgcccgtag ccacgaccga
     6661 gatcacgagc ggagcgttcg gccggcctat ctccctgaac gccgagccta tcgactgcga
     6721 cagggccatc ggtaggaagg tccacgagac gagccgtagg tagctggcgc cgtgcgcgac
     6781 gatctccgcc tgggcagcgt taccgatcgt catcatcgtc accaggctcg aaggcatgac
     6841 ctggcagacg gcgaagtaca gcgccgagac gccaagggtc atgatggcct tgaagcggta
     6901 ggcctggcgc atgccttcct cgtccttggc gccgcggtac tgcgacatgt atatgccgcc
     6961 ggcggccgag agggtcatca tcacgaccat gtacacgaag ttgacctggt tggccacgtt
     7021 gacgccggcc atcttggcgt cgccgagccc cgccaccatg aagttgtcta tcagcgacac
     7081 gaggctctgg atggcctgct gcgccatcac cggcaacgcg acggcgagca cttccttata
     7141 aaacgcggct ggcccgaaga gcggccggcg ttcaagcgta tggttcatcg ctcatgtata
     7201 gagaagataa aataaaagga caatagggac aggtcgacta taggcatctt tggacaggcg
     7261 cgtcgccttc gtcgccgcgc ccggccgccg agggcggccg ccgttccgtt cggctcccgg
     7321 tcggctccac gcgcccgggc gccgcgcgcg gcgcggccgc tagtccaccc ggcggcggta
     7381 gcgcgcatat aggaacgaga gccaggccgc gctaaccgcc aggccaagtc cgagcgcctc
     7441 aggcgaaccg acggcgacgc cggttcccgg cccgagcgac gccccgaggg cgaggcgcgc
     7501 cgcccagtac tgcggaaagg gccaggccag cgcccgggcc ggcagcggca gcaacaccgc
     7561 gagcggcgcc aggtcgaggg cgctcaaggc cttgagcgta gccaggccct ccaccttgtc
     7621 ggaggcgatg gcggccagga acagcgcgaa gaacggcgcc tggagggccc cgacgaccgc
     7681 gacgctagcg tcagctgcga acgaccggcc gcctagcccg aaggccggcc cgacggccag
     7741 gcccgcgacg agagccgccg tgcagaacag ggcgagccgc gcggccaggt agccggagcg
     7801 gccgaccggg ctcacggacc agtagggcga cacgccgtcg tctcggtcgt ccagcagcag
     7861 gaagcctgac atcatgccga agaaggtgcc ggggaacagg atcagcacga cgcgtatcag
     7921 gtccgcgtag tcgagtagca tgaaaccggt cgccttagcg atgaacgcgg caagcggggg
     7981 cagagccagc ctgagcgcta tcgccgccag gaacggcacg aacggcatga tgcagaggag
     8041 ggggtctctg ccgaccaggc gcaggtcggc ggctacgagc cgcgacccgg cggctacgag
     8101 ccgcgcgccc gtggccatgg atcgcgaccc ggcggccgct ggccgggtat cgttcgcgcg
     8161 catcctagtc gcccctcgcg gcgttcacgg ccggggccag cgcgaaccgg gctatggcca
     8221 ccgtccaggc ggccgcggcg agcgcgccga gggccagctc gccggccggc accggcgtat
     8281 acgcggcgcg cgcgaaggcc agcgacccgt cgccggggct caggtccgcc acgagcccga
     8341 accacggcgg caggtatcgc ccggccgcgt actggacggc cggcagagcc agtaccagga
     8401 acgccggtat cgagcccatc atgaaacggt tgacgctctt gagcctgagc gccagggtgg
     8461 cgccaagcag ggccgtcggc gcgcctccag ccacggccga cacggcgagc ggccatagcc
     8521 tgaagccagc gccgcgcgac gacgccgaca cgagagcggc catcgcgaac gacaggatcc
     8581 cgagatcgag cgccttgacg atcaggtagg cggatggccg cagcggcgag gcgaacaggg
     8641 agcggaaggt gccctgggcc aggtccaggc agacggaggc gccggcgaag aagaagcaga
     8701 agaaggtagg gtcgctccag gctaataccg ggaggactgc gtcggcccag gcgcgcggca
     8761 gggccgacag cgcggccgcg tacaggaccg ccacgaccag gtaggctatc gcgaagccgt
     8821 ggcgccacaa gaggcgcagg tcggcggcga acaacgcggc gttcatacga gttcctttcc
     8881 ggtcagcctg agaaagatgc cgtccaggct ggagtcgagg gtgcgcacgc cgagcaggcc
     8941 cggcttccgc agctccgcga tgaaggccgc gtcgtcggcc aggccgtcga gagggtactc
     9001 ggcttgggcg gtcttctcgc tggcgccgtc cgaggcgccg tccgaggcgc cagccgcgga
     9061 accgccggcc gcggctccca cctccggccc gggctcgcgg tcgacgacga cggcccgctt
     9121 gccgtagcgc cgcttgaggg cggcgggctc atcgcaggcg gcgatgcggc cgtccacgat
     9181 gaaggcgacg cggtcgcagg tggcgtcggc ggcggccatg tcgtgggtgg tcaggaatac
     9241 cgcggcgccg tccgcggcgc gctcgcgcac gatgtcgcgc acgacgcgcg cgttggccgg
     9301 gtccaggccg ctcgtcggct cgtccaggaa caggacgcgc gggcggtgga tgacggcccg
     9361 gatcaggttg agccgcatgc gcataccctt ggagtactcc gctaagcgcc agtccatcgc
     9421 gtcggccagg ccgagccgct cgaacagcgg gcgcgggtcc accttgggcc ccgggtagaa
     9481 cgacgcgaac agccgtacgt tctcgaggcc ggtcagtttc tggtagaagc aggggaactc
     9541 gaacgccgcg ccgatgccgg cccggtatcg ggaatcggcc ccggacgcct cgacgctccc
     9601 gactgtcgcc gagcccgagt agccgcgcag cgacccgagc aggatcttct gcgtggtgct
     9661 cttgccggcg ccgctcggcc cgaggaagcc gaacacctcg ccgggcgcca cggcgaagtc
     9721 gatgcctcgt atcgcctcgg acgtaccctt cggataggta aagcgcaggt ctcgaacccg
     9781 tatcatctcg tccttctttc ttcgtggcgg cgcgtatcgg aatagcggcg gagcgtatcg
     9841 gaatcgcggc ggagcgtatc ggaatcgcgg cggccgcctc gttcgtcgga gccttcccct
     9901 atggcgcctt ccgttccggc cagggccaag gccaggccta gggcgaccac cgcgaccgtc
     9961 gcggcgcgcg gcccctcggg caggttgcgc aggcccgtcg ccatcaggaa caccgcgtat
    10021 ccggcgccgg ccagctcggc cgccccgacg cccggcccga gcaggcccgc ggccgaccag
    10081 gcggccgcta tcgtcgccat cgcggcggcg tcggcctcga cgtggcgggc cacccgctcg
    10141 ggcggcaggg cgcgggtcag ccgctcgagc gtgcggccgt ccatcgaagt caccgccggg
    10201 tcgctcgtca ccttggcgaa ggccgcctgg aaggcggcgt gtactcgttc gaaggccgtt
    10261 ccgggcaagg ccggcgcggc ctcgaaaatg gcccgatagt cgcgctcgat gccttcgagc
    10321 gcgtccatga acagctcctc cttcgagtcg tagaacaggt agaagctacc cttggctatg
    10381 gaggccgccg ccgccagttc cgccacgctc gccgccccga gcccgccgcg cccgagcgcg
    10441 tcgacggccg ccgccagcag ccgttcccgg atcgcggcgc gctccctatc gtcgaacgcc
    10501 gtaggcatcg atacgtccct ccttcgcgct atgaccattt atacttatat ggtcatacgg
    10561 taatcgccat cgtggcccct gtcaagcctc gccgggccgt tcgtcgcagg gctttcccga
    10621 agccccggac gtggtatagt agccgcggga cgccgagcgt ccacggagga tgcccatgag
    10681 cttcttcaag cccttcgccc tcgagcgctt cttcgccaag cacgagttca gcgccaagta
    10741 cctgctctgt tcgtcggact gcgagtcctt cccggtgcgc gagctcttgg cgatggacga
    10801 gggctcgatt gaccggctac tcgacctcag gctcggctac accgagacca ggggggcgcc
    10861 gtcgctccgg gaggcgatcg cgggccacta cgcggggctc gggccggagg gcgtcttcgt
    10921 ccactcgggc gccgaggagg ccatcctcaa cctgtacctg gccgcgctga aagctggcga
    10981 ccgggtggtc gtcaattatc cctgctacca gtcgctcgcc gagcttcccc gctcgctcgg
    11041 ctgcgaggtg attccctggg agctacgcca ggtcgacgac ggccgcggca agtcgcgttg
    11101 gcggctcgac ccggacgagc taccggccct gtcgggcggc aaggcgacga tgatcgtgct
    11161 gaacgcgccc cataacccca ccggcgcctt gccgacccgc gacgagttcg aggcgatcgt
    11221 gtcgtacgcg cgtaagaccg gcgcgatcct gttcgtcgac gaggtgtatc gcaggctgga
    11281 gcgcgaccgc gcggccaggc taccgtcggt ctgcgaggcc tacgagaacg gcgtggccct
    11341 ggacgtgctc agcaagcact cgggcctcgc cggcctccga ataggctggc tagcgtcgcg
    11401 ccgggtcgac ctgctcgacg ccgtcgcggt cgtcaaggac tacaactcta tctgctcctc
    11461 ggcgccgagg agcagataga gttgtagtcc
//
LOCUS       DAOPHV010000014        11374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_6876_length_11374_cov_4.516212, whole genome shotgun sequence.
ACCESSION   DAOPHV010000014 DAOPHV010000000
VERSION     DAOPHV010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 11374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11374
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6876_length_11374_cov_4.516212"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLQ29_00800"
     CDS             <1..184
                     /locus_tag="PLQ29_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85209.1"
                     /translation="CVNKRGLSKAPWQLDAELAEIPAFYFDFKPEGLHERDDLMRLDT
                     QDLVAAFDAAASEGIR"
     gene            181..822
                     /locus_tag="PLQ29_00805"
     CDS             181..822
                     /locus_tag="PLQ29_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85210.1"
                     /translation="MNPYALVALVVLAIAATAQYYLGTRKNRFLTSRMSGALEELLKP
                     STTNYVNIGGSIGYNFAYSLSGDWTKAKGTMTLNARHSLLYLPFSLLIGVRDRFYVNV
                     FTKKKLRGEAHLVEASYLRRANIDGVDSMDRAEAEAGGKRFVLLWRGADLSGELRAVL
                     ESLGQPERLRHFCAYPETKTFFVHLRPKEGDIRDDLSAMLQRMPTFFVEAKEG"
     gene            825..1109
                     /locus_tag="PLQ29_00810"
     CDS             825..1109
                     /locus_tag="PLQ29_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85211.1"
                     /translation="MDLPMDVRVKFDAILDGVIEPQSELSLAELGLVKKITYYKADTT
                     IVVYMNYARPTVADCPACSVATDMMKSTIERDLSAALLEAFPGWTVRFDQ"
     gene            complement(1155..1337)
                     /locus_tag="PLQ29_00815"
     CDS             complement(1155..1337)
                     /locus_tag="PLQ29_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HPG85212.1"
                     /translation="MAGKFEWFKDKKDKYRFRLKAGNGQIIATSEAYESKDSCLNGIE
                     SVKKNAPDAPIVETEA"
     gene            1439..2110
                     /locus_tag="PLQ29_00820"
     CDS             1439..2110
                     /locus_tag="PLQ29_00820"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG85213.1"
                     /translation="MPKDCIHFAATALLSCAWLAACSWPRDPSVASEAAIGAYAQALE
                     RYSAGDMVGAAARAERSLGRSGRFLPALLLLGKASLLSGDDEKAVAALKRALAATPRA
                     GEAAVWLARAYRASGLAAEASALCAIAVSADPGSVTALRLMASLASDDERLADAKAYL
                     DRALEASAEVGMAFIDRASIRWASGDRTGALADLDAAVTVLPVGSNAQKAAVSLGQRI
                     REPTP"
     gene            2107..3564
                     /locus_tag="PLQ29_00825"
     CDS             2107..3564
                     /locus_tag="PLQ29_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPG85214.1"
                     /translation="MRTSQTLCAPVAAVALTLGACAGYYVDYRYATSVELDALASVRG
                     YPETIIAGEPLGVAEAAAYAIANDQALSSDREALDLREGRWRLAFRSFLPSVTMGASA
                     DETLAAVGAESLSRSMSASIEQALWDGGRLAARRALESVELGLARLELERSTYETGEG
                     AVASYRGVMTARARLEIQTAALDAARLSRAALASELGLGLASADELLEADLEIGDAEI
                     ALSQASLELSEAEGNLAYALGVDKLPPLADGGLGQRPELKLDADELWRHVVELSPELR
                     EARYRIVAKRAEARAAAAGWLPAIGLTASWRLSGDRFPLSSLSWSVGARVEFSGPLAK
                     VGMGLETGGDGRRERNAANTLSAEPVPDPAGIMDIRSARLALRVEEEAYAAALRELRV
                     RTMAATRAYEHSLARRRLCRDAARLASIRFDSVGLRRDLGRATTLEALRAGLERADAE
                     IDLLEACASIVEAEREVERLIGLPPDGLCAFVEGL"
     gene            3561..4919
                     /locus_tag="PLQ29_00830"
     CDS             3561..4919
                     /locus_tag="PLQ29_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPG85215.1"
                     /translation="MSSGFKRMWNSSAKALVASLALSTASCGASAAAATEEVLPVRVA
                     VATSLTLDDEIQGYGAIAYSRKVDLATAQSGVIASLPYREGEKVEKGDVVAVVTNPRV
                     LLALGIAENGVASASAELALAEARLFSGRLNAESRLLDIERERMELSLARLELREAER
                     KQADQEALYAAGSVAEETVRSGRFSLESAKGGIELIEREIASNLVGLRDEDLVARGLR
                     VPEGEAARAAALADLCTESLVAERDAAKASLEAAIKELEAAGLALAELTLVAPISGVV
                     GTLHLEEGERADSGSPVITLIGTDGLRAVFTVREALAVRLSPGLAATVRVDATGGEYA
                     AVVDFVSPMADSGSASFTARVAFVDDPKDLRPGMFARVSVVAGPPTVAVVIPSSSTLG
                     EGDQASAFVVSDGVVWRRSLTLGDVGPGGRVVLSGLKVGEVVVDEPGSRLEEGCRVSI
                     GR"
     gene            4900..6378
                     /locus_tag="PLQ29_00835"
     CDS             4900..6378
                     /locus_tag="PLQ29_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPG85216.1"
                     /translation="MYRSADEKPQGPPRGAAFIVALCACSLAQSACGLVDLRPVAVAT
                     HPSGPYEVVASRDETLWVMFPVAPSRIDAEEAVTVRSSTGPTDGDLSWDGLCLSWRPI
                     EALDPGSRYRFVLSGDVRMEDGRVADLALDLPFFVLRAGSMPRLVSSAPSDGASVAAS
                     ASGSTALVLRFSEPMDEASVESAFSLSPGPDYRFLWGEGGSLMSIRLLERLSPCVCYA
                     WSLGQDARAVDGAPIARAESGCFVTDLDAETPSVLATGPAALVDGRWVELSTDLSETD
                     AGDSVAVRFSEPMDVDSVVGAITMSPARSGQVVAASTELFVYTPREPWTPEEYQRLVV
                     RTAAKDTSGLALPRERVFGFTPRTPYLELSSVSTLVGEESPSPGEGGAFAVTIGDPPE
                     GELTLILRFSDSFAAVAMESATRSVTVSAFFPSGLRSPRLRSAWWPSADTLSMTWEGL
                     EASDADSERYYRLVVRGGEDGLASQRGLRLRADLAQILEAKP"
     gene            6375..7931
                     /locus_tag="PLQ29_00840"
     CDS             6375..7931
                     /locus_tag="PLQ29_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPG85217.1"
                     /translation="MRTMARVSTRLAISLATLLAMLLASANACSLLVPYSFEVVAWSP
                     GQERIDEAGGIIVGVEFSREADRPSSESAFSLSVDGEAVLGAFSWLGARMGFTPYAPL
                     RDGADYELRVGTGAMDQAGVSLERAFEARFSTKAERSRPRFVSSDPPDGGRMVGELGT
                     LTLEFDEAIDPIGFRSSLSIAPRARGSWRLVEGGRRIEFRPTEPWARSVDYTCEADAG
                     LRDEEGNEIGRSVVVRFVAGLEGVAPEVTAAAAVDAEGEVVALLEADEAEDGAYTLNR
                     GWESRWRLRMDFSEPVELAGLASALACDSGPKPLIETIGAYSASVLWRFDEPPEWNGV
                     FTLRVESGVEDQDGDSSDSDIVYRIVADGPGSRPPRFVGLRLPLAPGAEAADRELAAF
                     ALDEPFSTLAIAGGDGRYPIGVPTGTSIELYLELAEGASIDLLSTMGAFSFSATNGSL
                     EFDALRVVAGGFDYAEPHPAWSGLDIVRIDGSMTNRADCGLVTFSLDGGYADSWGNAA
                     KEDQCLSLQK"
     gene            7936..11010
                     /locus_tag="PLQ29_00845"
     CDS             7936..11010
                     /locus_tag="PLQ29_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPG85218.1"
                     /translation="MKRVITSCAGRPVAVAMGLVAIMMAGIGAAIDLPRRSLPDVSVP
                     RVLVEATMPGMSAAAMRSLVTCPLEDALASADGLIGSSSVSRDGRTVITLDFNWGEDA
                     ARMASNVRDIIDATYPGLPEGATKPSVTADDPAASPLVTVTMAPMAGDLAFAKRMAER
                     EGRARLRRVVGVGAVTVVGGRDRELAVAVDERKAAARGMTARDVASALAAECADVPLG
                     SVEGGGLELVVIADGRPKTVAELEAVVAVSPAGSFRLSELARVYERDAPADSLYVLDG
                     IETVALEVYLRPGADPVATAREVVLVAEAMAVEFMDELAIRTVGDSSTAVAASIRELA
                     WAAVIGSIAAAVVLYAMLRDARCAVMVAVSIPICIAATLAALSLAGRSLNRMSLSGLA
                     LAVGIISDNAVVTLDALSTVGETMPRGTSRRMYPTPAEIGEAASSVVGGTFGGMVTTV
                     AVFMPIFYLPGAIGSIFGDLAFAVVVASACGWVCAMTILPAAYRLFGTAGSGGRPPAR
                     LAASYARSLSTAMRRPSRLIICAAIAAAIGVALAATRDVSFMPRGAVSELLVTARFEP
                     GSDAEGIRPEAISLSAILSRVDGVVSTRGWAGAEVGDLSRLADPAYGPERLSLLCALS
                     SKADTETIAREIAAAARAALPPSASLTVAEPPDPAAAIMGLVEGSTIVVNADSVEAAK
                     AAADAFESYLSDLAGDALASVVRSPDGQAGRVLASPDRQRIAGLGVKLAEVEAVIRTA
                     TEGVRVGNMRSDGEDTDIVVFAGGAGSADRGGSLESLRSITVEAGGSAPIHASTVATF
                     DLAYSAAALARLDRRDAIYLTPVAAVSGRGALEAAVERAMIAYGSGARSSVHRSGGSA
                     FGRYAEAMAWTLALVLVLLYLVIGSQFESFVAPLVIMAAIPLALAGSGPTMLIAGVGL
                     DSGSIMGLVALFGTVVNSAILLYEACAARLAAGEAPDSAAFLGAIDRLRPVLATTMTT
                     LVALVPLCLNPEASPQRSMAVSMIGGMAASTALTLFITPTVLAALARRRAAA"
     gene            11007..>11374
                     /locus_tag="PLQ29_00850"
     CDS             11007..>11374
                     /locus_tag="PLQ29_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPG85219.1"
                     /translation="MSDSRARWYGKPVAAVCVAAAAVALSARTLAVTEFGGSPGSGGS
                     VCVVSLSQYGTNAREMESLVAIPLEDMLASLPGVIGTSSSCEYGEARVVARFAEGRDG
                     TEAVSAAAQRLYESLPRSVQR"
BASE COUNT         1749 a         3670 c         4000 g         1955 t
ORIGIN      
        1 ctgcgtcaat aagcgcggcc tgtccaaggc gccctggcag ctcgacgccg agctggcgga
       61 gatcccggcg ttctacttcg acttcaagcc ggaaggcttg cacgagcgtg acgacctcat
      121 gaggctcgac acccaagatt tggtggccgc tttcgacgcg gccgcctcgg aaggcatcag
      181 atgaatccat acgcactcgt ggctctcgtc gtgctggcca tagcggcgac ggcccaatac
      241 tacctgggta ccaggaagaa ccgcttcctg acctcgcgca tgtccggagc ccttgaggag
      301 ctgctcaagc cgagcacgac caattacgtg aacatcggcg gctccatcgg ctacaatttc
      361 gcgtactcgc tgtcgggcga ctggaccaag gccaaaggca cgatgaccct caatgcccgc
      421 cattcgctcc tgtacctgcc attctccctg cttatcgggg tccgcgaccg gttctacgtc
      481 aatgtcttca cgaagaagaa actgcgcggc gaggcgcacc tggtcgaggc ctcgtacctg
      541 cggcgcgcca acatcgatgg cgtcgactcg atggaccgcg ccgaggcgga ggccgggggc
      601 aagcgcttcg tcctgctgtg gcgcggcgcc gacctgtcag gcgagttgcg ggcggtgctc
      661 gagtcgctcg ggcagccgga gcgcttgagg catttctgcg cctatcccga aaccaagacc
      721 ttcttcgtcc acctgcggcc gaaggaaggg gacatacggg acgatctttc ggccatgcta
      781 cagcgcatgc cgacgttctt cgtagaagct aaggaaggct gaccatggat ttgccgatgg
      841 acgtcagggt caagttcgac gctatactcg acggggtgat cgagccgcaa tccgagttgt
      901 cgctggccga gctcggccta gtcaagaaga taacttacta taaggcggat acgaccatcg
      961 tggtctacat gaactacgcc agacctactg tggcggactg cccggcttgc agcgttgcga
     1021 ccgatatgat gaagtcgaca atagagcgcg acctgagcgc ggctctgctc gaggcattcc
     1081 cgggctggac cgtccgcttc gatcagtagc gccggcctcg acgagagcgg cggccgaggt
     1141 ggccgccgct cgcatcatgc ctccgtctcg acgatcggcg cgtcgggagc gttcttcttg
     1201 acgctctcta tgccgtttaa gcacgagtcc ttcgattcgt aggcctcgct cgtcgcgatg
     1261 atctggccgt tgcccgcctt gagcctgaaa cgatacttgt cttttttgtc cttgaaccat
     1321 tcgaattttc cagccatcga tacactcctt catgaagatg tgaaattatt ctaatacgtc
     1381 atcccttccg gcgcaagcga gacggcccca acgggcgtgg atccggcacg gaggagccat
     1441 gccaaaggat tgcatccact tcgccgccac ggctctgcta tcgtgcgcct ggctagccgc
     1501 ctgttcgtgg cctcgggatc cgagcgtggc cagcgaggcg gccatcggcg cgtacgccca
     1561 ggcgctcgag cggtattccg ccggcgacat ggtcggggcg gcggctcggg ccgagcggtc
     1621 gctcgggcgc tccgggcgct tcttaccggc cttgctcctg ctcggaaagg cgtcattgct
     1681 gtcgggggac gacgagaagg cggtagcggc gctcaaacgg gctctagcgg cgacgcccag
     1741 ggcgggagag gcggccgtat ggctagccag ggcctatcgg gcgagcggct tggccgccga
     1801 ggcgtcggca ctctgcgcca tagccgtatc ggctgatccc ggatcggtta cggccctcag
     1861 actcatggcc agcctcgcat ccgatgacga gcggctcgcc gatgccaagg cgtacctcga
     1921 ccgcgcgctc gaggcgtccg cggaggtcgg catggccttc atcgaccgcg cgtcgatacg
     1981 ctgggcgtcc ggggaccgca ccggcgccct cgccgacctc gacgcggcgg ttacggtact
     2041 tccggtcggg tcgaacgcgc agaaggcggc cgtcagcctg ggacaacgga tacgggagcc
     2101 cacgccatga ggacatcgca gacgctatgc gcgccggtcg cggccgtcgc gctgacgctc
     2161 ggagcgtgcg ccgggtacta tgtcgattat cgctacgcga cgtcggttga gctcgacgcc
     2221 ttggcttccg tacgcggcta tccggagacc atcatcgcgg gcgagccgct gggcgtcgcg
     2281 gaagccgcgg cttacgccat agccaacgat caggcgctct cctcggatcg tgaggccctc
     2341 gatctgcgcg aaggccgatg gaggctcgcg ttccgctcgt tcctcccatc agtcacgatg
     2401 ggcgcgagcg ccgacgagac cctggccgcc gtcggcgccg agtcgctgtc ccgttccatg
     2461 tcggccagca tcgagcaggc gctatgggac ggcggacggc tagccgcgcg tcgagctctc
     2521 gagtcggtcg agttgggctt ggccagactc gagttggagc gatcgaccta cgagaccggc
     2581 gagggcgcgg tcgcctcgta tcgcggggtc atgacggcga gggcacgcct ggagatccag
     2641 acagcggcct tggacgcggc tcgcctgagc cgagccgcgc tggcgtccga gctgggtctt
     2701 ggactagcct cggccgacga gcttctcgaa gccgacctgg agataggcga tgccgagata
     2761 gccttatcgc aggcgagtct ggagttgagc gaggcggaag ggaaccttgc gtacgcgctc
     2821 ggcgtcgaca agctacctcc gttggcggac ggcggcctcg gccagcggcc cgagcttaag
     2881 ttggacgcgg acgaactctg gcggcacgtc gtcgagctgt ctcccgaact cagggaggcg
     2941 cgttaccgaa tcgtggcgaa acgagccgag gccagggccg ccgccgcggg atggctcccg
     3001 gccataggcc tgacggcgtc ctggcgacta agcggcgatc ggttccccct cagctctcta
     3061 tcgtggtccg tcggggcgag ggtcgaattc tccggacccc tggccaaggt cgggatgggc
     3121 ttggaaacgg gcggcgacgg aaggcgcgag aggaacgcag ccaatacgct atcggcggag
     3181 ccggtgccgg atcccgcggg catcatggat atacggagcg cccgcttggc cctgcgcgtc
     3241 gaggaggagg cctacgcggc ggctttacgc gagctgcgag tcaggaccat ggctgcgact
     3301 agagcctatg aacactcgtt ggccaggcgg cggctatgcc gagacgctgc gcgcttggcg
     3361 tcgatcaggt tcgactcggt gggcctgaga cgggacttgg gccgggcgac gacgctcgag
     3421 gccctacgcg ccgggctaga acgagctgac gccgagatcg atctgctcga ggcctgcgcc
     3481 tcgatcgtcg aggcggagcg ggaggtcgag cggctaatcg gactgccgcc cgatggccta
     3541 tgcgctttcg tggagggact atgagctccg ggttcaaacg gatgtggaac tcgagcgcca
     3601 aggcgctcgt cgcctcgctc gccctgtcga cggcctcctg cggggcgagc gcggccgccg
     3661 cgaccgagga agtcctgccg gttagggtcg cggtagcgac ctccctcacg ctcgatgacg
     3721 agatccaggg gtacggggcc atcgcgtact cgaggaaagt cgacctggct accgcccagt
     3781 ccggggtaat cgcatcctta ccgtacaggg agggcgagaa ggtagagaag ggggacgtgg
     3841 tcgccgtcgt aacgaacccc cgcgttctac tggccctggg aatagccgag aacggcgtgg
     3901 cgagcgccag cgccgagctc gccttggcgg aggcccgcct gttcagcggt cggctgaacg
     3961 ccgagtcgcg gctactcgac atcgagcgag agagaatgga gctgagcctc gccaggctcg
     4021 agctacgcga ggcggagcgc aagcaggcgg accaggaagc gctgtatgcg gcggggagcg
     4081 tcgccgagga gacggtacga tccggccgct tctcgctaga gtcggctaag ggcggcatag
     4141 agctgatcga acgtgagatc gcgtcgaacc tcgtcgggtt gcgcgacgag gacctcgtgg
     4201 ccaggggcct ccgcgtaccc gagggggaag cggctcgggc cgccgccctg gccgacctat
     4261 gcacggagag cctggtcgcc gagcgcgacg cggccaaggc ctcgctcgag gcggccatca
     4321 aggagctcga ggccgccggc ttggcgttgg ccgagctgac cctagtggcg ccgatcagcg
     4381 gcgtcgtcgg caccctgcac ctcgaggagg gcgagcgcgc cgatagtggg agcccggtga
     4441 taacgctgat agggacggac ggcctgaggg ctgtattcac cgttcgcgag gcgctggcgg
     4501 tacgcttgtc gcccggcctg gctgccacgg tccgcgtcga cgcgaccgga ggcgagtacg
     4561 ccgcggtggt ggacttcgtc tcgccgatgg ctgattcggg cagcgcgtcg ttcacggcgc
     4621 gcgtcgcgtt cgtcgacgat cccaaggatc tgaggcccgg catgttcgcg cgggtaagcg
     4681 tggtagcggg tccgccgacc gtggcggtgg tcatcccgtc gtcgtcgacg ctcggcgaag
     4741 gcgaccaggc gagcgccttc gtggtcagcg acggcgttgt atggaggagg tcgctcacgc
     4801 tcggcgacgt cggccccggc ggccgcgtgg tgttgtccgg cctgaaagtc ggcgaggtgg
     4861 tagtcgacga gccgggttcg aggctcgagg agggatgccg tgtatcgatc ggccgatgag
     4921 aagccccagg ggccgccgcg cggagctgcc ttcatcgtcg cgctctgcgc gtgctcgctc
     4981 gctcagagcg cttgcgggct cgtcgacttg aggccggtcg cggtagcgac ccacccttcg
     5041 ggaccatacg aagtcgtcgc ctcgagggac gagaccctgt gggtcatgtt cccggttgcc
     5101 ccgtcgcgaa tagacgccga ggaggctgtg accgttcgct cctcaaccgg gccgaccgac
     5161 ggcgacctat cctgggacgg cctatgcctg agctggcgac cgatcgaagc gctcgatccg
     5221 ggatcgcgct accgtttcgt gctgtcgggg gacgtgcgca tggaagacgg cagggtggcg
     5281 gacttggcgc tggaccttcc gttcttcgtc ctgcgagccg gctccatgcc tcgcctcgta
     5341 tcctccgcac catcggatgg agcgagtgtc gcggcgtcgg catccggttc cacggccctc
     5401 gtgctacgct tctcggagcc gatggacgag gctagcgtcg agtcagcctt ctcgctcagc
     5461 ccgggaccgg actaccgttt cctttggggc gaaggcgggt cgctcatgtc gatccgcctc
     5521 cttgagcggc tttcgccatg cgtctgctac gcttggtcct taggccagga cgcgcgggcc
     5581 gtcgatggcg ctccgatagc cagggcggaa tctggctgct tcgtaaccga cctcgatgcg
     5641 gagacgccct cggtgctcgc caccgggcct gccgctctcg tcgacgggcg ctgggtcgag
     5701 ctatcgactg acctgtccga gacggacgcg ggcgactcgg tcgccgtcag gttctctgag
     5761 cccatggacg tcgatagtgt cgtcggcgca ataacgatgt cgccggctcg ttccggccag
     5821 gtcgtcgcgg cgtcaacgga gctgttcgtc tatacccctc gagagccttg gacgcccgag
     5881 gaataccagc gcctcgtcgt gaggaccgcg gccaaggaca cgtccgggct cgccctgccg
     5941 cgggaacgcg tcttcggttt cacgccgcgg acgccgtacc tcgagctatc gagcgtctcg
     6001 accctagtcg gtgaggagag tccgtcgccg ggcgaagggg gagcgttcgc cgtgaccata
     6061 ggcgacccgc cggaaggcga actgacactg atcctcagat tctcggacag cttcgcggcc
     6121 gtcgcgatgg aatcggctac gaggtcggtg acggtatcgg cctttttccc gtccgggctc
     6181 cggtcgccgc gcctgaggag cgcctggtgg ccgtcggccg ataccctgtc gatgacctgg
     6241 gaggggttgg aggcgagcga cgccgacagc gagcggtact accgcctggt cgtgcggggc
     6301 ggagaagacg gacttgcgag ccagcgcggc ctacgcctgc gggccgacct ggcgcagatc
     6361 ctggaggcta aaccatgaga acgatggccc gcgtatcgac gcggttagcg atatccctgg
     6421 cgacgctcct ggcgatgctc ctggcgtcgg ccaacgcctg ctctctgctg gtaccgtact
     6481 ccttcgaggt cgtcgcctgg tcgcctggtc aggagaggat cgacgaagcg ggcgggatta
     6541 tcgtcggcgt cgaattcagc cgagaggccg acaggcctag ctctgaaagc gccttctcgt
     6601 tgagcgtcga cggcgaggcc gtcctcggtg cgttctcctg gctgggcgca cggatgggct
     6661 tcacgccgta tgcgccgcta cgagacggcg ccgactacga gctccgcgtc ggtacgggcg
     6721 cgatggacca agccggcgta tcgctcgagc gagccttcga ggcccgcttc tccacgaagg
     6781 ccgagcgctc aaggccgcgg ttcgtgtcga gcgatccgcc ggacgggggg cgcatggtcg
     6841 gcgagcttgg aacgctcacg ctcgaattcg acgaggcaat cgacccgatc gggttccgct
     6901 ccagcctgtc gatcgcgcct cgggcgcgcg gctcctggcg gctcgtcgag ggcggacgac
     6961 ggatcgagtt caggccgacc gaaccctggg cgcgctcggt cgactatacg tgcgaggcgg
     7021 acgccggcct gcgcgacgag gagggcaacg agatcggccg ctcggtagtc gtacgcttcg
     7081 tcgccgggct cgaaggcgtc gcgcccgaag tgacggccgc cgccgccgtc gacgccgaag
     7141 gagaggttgt ggccctgctc gaggccgacg aggccgagga cggcgcgtat accttgaacc
     7201 gcggatggga gtcgcgttgg cgcctccgga tggatttttc cgaaccggtc gagctagccg
     7261 gcctggcctc ggctctcgcg tgcgattccg gtccgaaacc gctcatcgag acgataggag
     7321 cgtattcggc cagtgtgctc tggcgcttcg acgagccgcc ggaatggaac ggcgtattca
     7381 cactgcgagt cgagtccggg gtcgaagacc aggacggcga ctcgtccgac tccgacatcg
     7441 tgtaccgcat cgtggccgac gggcccggct cgaggccgcc tcgttttgtc ggccttcggc
     7501 taccgctggc gccgggggct gaggcggcgg atcgcgagct cgcggctttc gcgctggacg
     7561 agccgttttc taccttggcg atagctggcg gcgatggacg ctatccgatc ggcgtcccga
     7621 cgggcacctc gatcgagctg tacctcgagc tggcagaggg cgcgagcatc gacctccttt
     7681 cgacgatggg cgcgttctca ttttcggcga cgaacggctc gctcgagttc gacgcgctga
     7741 gggtagtagc cggcggtttc gactacgcgg agcctcaccc ggcttggtcc gggctggaca
     7801 tagtccgcat cgacggctcg atgacgaacc gcgccgattg tggcctcgtc accttcagtc
     7861 tcgatggcgg ctacgccgat tcctggggca acgcggccaa ggaagaccaa tgcctgtcac
     7921 tacagaaata gcgggatgaa gcgcgtcatc acgtcgtgcg cgggccggcc ggtggccgtg
     7981 gctatgggct tggtcgccat catgatggcc ggcatcgggg ccgcgataga cctgcccagg
     8041 cggagcctgc ccgatgtgtc ggtaccacgc gtcctcgtcg aggcgacgat gcccggtatg
     8101 agcgcggcgg cgatgcgctc cctcgtcacc tgtccgctcg aggacgcgct cgcctcggcg
     8161 gacgggctca tcgggtcgtc gagcgttagc cgggacggac gtaccgtcat caccctcgat
     8221 ttcaactggg gcgaggacgc agcgaggatg gcgtcgaacg tacgcgatat catcgatgcg
     8281 acctatccgg ggttgccgga aggcgcgacc aagccaagcg ttactgccga tgacccggcg
     8341 gcgtcgccgc tcgtcacggt gactatggcc ccgatggccg gggacctggc cttcgccaag
     8401 cgcatggccg aacgcgaggg ccgggcgaga ttgcggcgcg tggtaggcgt cggcgccgtg
     8461 accgtggtcg gcggcaggga tcgagagcta gccgtcgcgg tggacgagcg taaggcggcc
     8521 gcgcggggca tgacggcaag ggacgtggcc tcggcgctgg ccgccgagtg cgccgacgtc
     8581 ccgctcggtt cggtcgaggg cggaggactc gagctcgtcg tcatagcgga tggtcggccg
     8641 aagaccgtcg ccgagcttga agccgtggta gccgtctctc ccgccggctc ttttcgccta
     8701 tccgagctgg cacgggtcta tgagcgagac gcgccggccg atagtctcta cgtgctcgac
     8761 ggcatcgaga cggtggcgct cgaggtctac cttcgtccag gcgccgaccc ggtggctacg
     8821 gctcgcgagg tggtactggt ggccgaggcg atggcggtcg aattcatgga cgagctggcc
     8881 attagaacgg tcggtgactc gtcgacggcc gtagcggcct cgatacggga actggcctgg
     8941 gcggccgtca tcggctcgat cgcggccgcc gtcgtgctgt acgccatgct ccgggatgcc
     9001 cgctgcgccg tcatggtcgc cgtatcaatc ccgatatgca tcgccgcaac cctggccgct
     9061 ttgtcgctgg cgggccgatc gctcaaccgc atgagcctgt ccggcctcgc cctggccgtg
     9121 ggtatcatat cggacaacgc ggtggttacc ctggatgccc tgtcaacggt gggagagacg
     9181 atgccgcgcg gcaccagtcg gcgaatgtac ccgactccgg ccgaaattgg cgaggccgct
     9241 tcctcggtag tcggcggaac gttcggcggc atggtaacga ccgtggccgt gttcatgccc
     9301 atcttctact tgccgggcgc catcggctcc attttcggcg acctggcctt cgccgtcgtc
     9361 gtcgccagcg cctgcggctg ggtctgcgca atgaccatac taccggcggc gtataggctc
     9421 ttcggaacgg ccggctcggg ggggcggccg ccggccaggc tcgcggcatc gtacgcgcgc
     9481 tcgctgtcaa cggctatgcg caggccgtcg aggctcatca tctgcgccgc tatcgcagcc
     9541 gcgatcggag tcgccttggc ggccacgaga gacgtatcgt tcatgccgcg cggagccgtc
     9601 tccgaacttc tcgtgaccgc tcgattcgag cccggtagcg acgccgaagg catccggccg
     9661 gaggcaatat cgttaagcgc catcctatcg agggtcgacg gcgtcgtgtc gacgaggggt
     9721 tgggccggag ccgaggtcgg cgacctgtcg aggctcgccg atcccgcgta tgggcccgag
     9781 cgcctgagcc tcctctgcgc cctatcctcg aaggcggaca ccgaaacgat agccagggag
     9841 atagcggccg ccgcgagggc tgccctgcct ccttcagcct cgctaaccgt ggcggagcct
     9901 ccggatccgg cggccgccat catggggctc gtcgagggtt cgaccatcgt cgtgaacgcc
     9961 gacagcgtcg aggcggccaa ggcggccgcg gacgcgttcg agtcctacct gtccgacctg
    10021 gccggagacg cgctcgcttc ggtcgtacgg tcgccagatg gccaggccgg acgagtcctg
    10081 gccagccctg ataggcaacg gatagccggc cttggcgtca agctagcgga ggtcgaagcc
    10141 gtcatcagga ccgctaccga gggcgtgcgg gttgggaaca tgcgttccga cggcgaggat
    10201 acggatatcg tcgtcttcgc aggcggagcc ggcagcgcgg atcgcggagg gagcctcgag
    10261 tcactgcgct caatcaccgt cgaagccggg ggctccgcgc ccatacacgc ctcgaccgtc
    10321 gcgaccttcg acctggccta cagcgccgcc gcgctcgcca ggctggaccg gcgggacgcg
    10381 atctacttga cgccggtcgc cgccgtgtcc ggtcgcggcg cgctcgaagc ggccgtcgag
    10441 agggccatga tcgcgtacgg cagcggcgcc aggtccagtg tccatcgttc cggcggctcc
    10501 gccttcggtc gctacgccga ggctatggca tggacgctcg cccttgtgct ggtccttcta
    10561 tacttggtga tcggtagcca gttcgagtct ttcgtggcgc cgttggtcat catggcagcc
    10621 attcctttgg ccctggccgg atcgggtccg acgatgctga tcgccggcgt cggcctcgac
    10681 tcggggtcga tcatgggcct cgtcgccctg ttcggcacgg tcgtcaatag cgcgatactg
    10741 ctctacgagg cctgcgcggc acggctcgcg gccggcgagg cgcccgatag cgccgcgttc
    10801 ctaggggcca tagacaggtt gaggccggta ctggcgacga cgatgacgac cctggtcgcc
    10861 ctcgttccgc tctgcctgaa tccagaggcg tcgccccagc gttccatggc cgtgtcgatg
    10921 ataggcggca tggcggcgtc caccgcgctt accctgttca taacgccgac cgtcctggcc
    10981 gccctggctc gacgaagggc cgcggcatga gcgattcccg cgcccgctgg tacggaaagc
    11041 cggtagcggc ggtctgcgtc gccgccgccg ccgtagcctt gtcggctagg acgctcgccg
    11101 tgaccgaatt tggaggctcg cccggctcgg gtggaagcgt ctgcgtcgtt agcttgagcc
    11161 agtacggcac gaacgcccgc gagatggaga gcctcgtcgc tataccgctc gaggacatgt
    11221 tggcctcgct gccgggtgtc atcggcacgt cgagttcgtg cgaatacggc gaagccaggg
    11281 tggtagcgcg cttcgccgaa ggccgcgacg gaaccgaggc ggtcagcgcg gcggcccagc
    11341 gcctatacga atccttgccg cgctcggtgc agcg
//
LOCUS       DAOPHV010000015        11302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_6948_length_11302_cov_4.198986, whole genome shotgun sequence.
ACCESSION   DAOPHV010000015 DAOPHV010000000
VERSION     DAOPHV010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 11302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11302
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6948_length_11302_cov_4.198986"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..486)
                     /gene="flgN"
                     /locus_tag="PLQ29_00855"
     CDS             complement(10..486)
                     /gene="flgN"
                     /locus_tag="PLQ29_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HPG85220.1"
                     /translation="MPPDGKLSRQELDERVAVLKRFRELLVRQRDKFNEYMSLLERQR
                     TDIESGDVDALVAHVELEQGIVSEIFAVQKVIEPLEDMYRSAYRGAEPEGVAELRSTL
                     SELKDEVVVRNSENRALLKQRMDMLRHEIMSVGNPYAKRKSVYGSAAEPRALDIRG"
     gene            complement(495..929)
                     /gene="fliS"
                     /locus_tag="PLQ29_00860"
     CDS             complement(495..929)
                     /gene="fliS"
                     /locus_tag="PLQ29_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HPG85221.1"
                     /translation="MTQNPLAAYKETRIRTAGPGQLVVMLYEEAVKQCDRAVDFLDTD
                     LKKKPANIEKVNQAICKVQDIVTELMASLDFEAGGDIARDLFSLYVYFNRELTEANIA
                     KDVGRVRAIRAMLAELSSAWAVAATKAQGSGDERRSGVNIAG"
     gene            complement(999..1484)
                     /locus_tag="PLQ29_00865"
     CDS             complement(999..1484)
                     /locus_tag="PLQ29_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPG85222.1"
                     /translation="MDQLFDRFGDLLKSWVQSDPGSSRPFSDPFAGKFSKSSGDPFLD
                     DAMDELDAFLNDDRESQERLRREREARARAEERAARPAAASGPPAKLVAAYKTLGLAY
                     GAPFDQVRAAYKRLLKEHHPDRHGSSPEAQLKATETSARINDAYRIIETWRDTGTLGD
                     E"
     gene            1557..2519
                     /locus_tag="PLQ29_00870"
     CDS             1557..2519
                     /locus_tag="PLQ29_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPG85223.1"
                     /translation="MSGFKPVAAVYGGANIDIQARSRGPYRAGDSNPGEASITHGGVG
                     RNIAENLAGLGVPTELVTVFGGDEQSSGLAEGCRRVGIEVSSSLFLPSEPCSRYVCLL
                     DRDGTLVGAVAAMDSLEAFGPDQLASRFGPGDAAEVVVVDANLPPETVALAAGRWKDK
                     PLILDTVSVAKARRVAPVVGLFGLVKPNRAEARVLLSLDPSERPVDPEAEAVGLTRGL
                     LALGVREAFVSLGARGFLWASAAGAGFARPLAMPVVNVSGAGDAATAALAWAASQGLD
                     TETKARYAVAAASVCAADERAVAAGVSADRLSALIRGVTCERIA"
     gene            2503..3417
                     /locus_tag="PLQ29_00875"
                     /pseudo
     CDS             2503..3417
                     /locus_tag="PLQ29_00875"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
     gene            3414..6446
                     /locus_tag="PLQ29_00880"
     CDS             3414..6446
                     /locus_tag="PLQ29_00880"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPG85224.1"
                     /translation="MTRGRRARGLASLACLAALAAGAVGPAAAADVVRFVNLGLGDGL
                     ANASVSSMVQDAAGFIWIGTQGGLHRWDGLSFTLYENEPFDPESLPHNLIQTLFLDED
                     GETLWIGTYGGLSRFDTRTGVFSSWAHDPADPGSLANDVVVSIARDAEGRLWVGTLGG
                     LDRMHGDSFVHYEASPEAEGALASGVIRALYRDSRGWLWVGTSGGGLYRYLPGTDSFL
                     SVRSASSGLPSDYVFSIREDGEGRLWLALWFYGLSRYDPVGGTFENRRLEDGRAYFVN
                     ADLDGLVYVGTWGGGLFELSLDDGAIRRFTSDGERRWSLPHDTMYSMLVDRAGDAWVG
                     TNGGGFSMLLHDEAGMSLYEHDPSDPGSLSSGKSISVLEDRRGRLWVGAYNGGVDRLD
                     PGSSSFRRYRHDPDDERSLPNDIVVNIYEDGRGGLWFLTNDGLARYDEGRDAFERVRR
                     DRSSTEGDAVRILYDMVEEPGTGRYWIATYTSGLELWDPVDGTVERYPSVPGDPSTPS
                     DNLVYAIAYDGRGRLWAATNAGLCRYEGDGRFVRYEASKTDRSKLPSRSLRDVYVDSL
                     GRLWVSTSGGGVARYVEESDSFEHWTKRDGLQSNIVHGIVEAADGSIWAVSTSGLSVF
                     DAAGGRWRPFSDQGRLRYGEFTSGRFASRSGLLYFGAVNALYVIDPAAIAGSPDAPPI
                     RFTSIKSLNEELDTGLSPWFVDRVRLGWSDPHLSLSFAALDYADPTRVQYAYKLEGFD
                     EDWVYSGARSYASYTNLPGGRSYSFKVRAATAIGDWDGEATELFIEVDKAPWLRWWAI
                     LLYLVAAGLVAFTVRLYASRAMLKSRVGELSALKGQLESANVKLEALAAHDGLTGLHN
                     RRAFNDELAARWQAAGATGEPLAALMIDIDFFKPYNDRYGHQAGDACLAAIAAAVKAA
                     LDRPHDSASRYGGEEFVALLPGTDAAGAARVAEKVRAAIEALGIPHGGSSVASAVTAS
                     VGYASLRPEPGSPPGRLVDLADEALYRAKNGGRNRVSP"
     gene            6496..8310
                     /locus_tag="PLQ29_00885"
     CDS             6496..8310
                     /locus_tag="PLQ29_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG85225.1"
                     /translation="MASKGSASKYDESKIKTLSSLEHIRLRTGMYIGRLGTGANPDDG
                     IYVLLKEVVDNAVDEFIMGCGDRVTVTVQDGSVSVRDRGRGIPLGKVIECVSVINTGA
                     KYSDDVFQFSVGLNGVGTKAVNALSSSFRVVSYREGQFFEACFERGTLMSKREGQARR
                     EPNGTLVEFTPDAQMFGDYAFNMEFVEKRMRHYAYLNSGLTLELNGQEFASQNGLLDL
                     LKDETGDDVLYEAGYWRGERIEFSFTHSNNYGEEYFSFVNGQFTSDGGTHLSAFREGF
                     LKAVNEFFRASYKGEDIREGLAAAVAVKLANPMFESQTKNKLGNGDIKPWIIQEVVRG
                     VDDYLRRNPTAAKRLEEKIAANERLRTELNVVKKEAREAAKKIELKIPKLKDCKLHLG
                     DGPDGDRSTIFLTEGQSATGSMVSCRDVMTQAIFSLRGKVENMHARKRVAIYKNEELY
                     NLMMALGIENDAGGLRYARIVIATDADYDGFHIRNLLLTFFLTFFEELVTAGRVFILE
                     TPIFRVRTKKETRYCYSPKERDEAVGALGSGSEVTRFKGLGEISPSEFGQFIGDDIRL
                     LSVDVQSLKSVPEVLAFYMGKNTPERRDFIMKNLKNDL"
     gene            8318..10210
                     /locus_tag="PLQ29_00890"
                     /pseudo
     CDS             8318..10210
                     /locus_tag="PLQ29_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
     gene            complement(10618..>11302)
                     /locus_tag="PLQ29_00895"
     CDS             complement(10618..>11302)
                     /locus_tag="PLQ29_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG85226.1"
                     /translation="DDGRSRIEEVTSAVSAIAERSGAMLDAVEVIAGIAKSTNLLAMN
                     AAIEAAHAGDAGKGFSVVADEIRRLSEESAERSREIAAALDQVKLSIDEAVGSSGSAT
                     EAFESVQGGVRQVSDLMEEMENAMREQAAGSSQISTAMSAMQADGIRVRDYSGELGAK
                     SAALADTAARLTEANASVSAEVGAMAERVRGAAGAIASVLEHTMANGRLISEIAGEIA
                     GFRVIDERP"
BASE COUNT         1964 a         3795 c         3675 g         1868 t
ORIGIN      
        1 gagtaaggct cagccccgta tgtcgagggc tcgcggctcc gcggccgagc cgtagacgct
       61 cttccgcttg gcgtatggat tgcccacgct catgatctcg tggcgcaaca tatccatacg
      121 ctgcttgagg agagcgcggt tctcggaatt cctgacgacg acctcgtcct tgagttcgga
      181 tagggtggag cgtagctcgg cgacgccttc gggctcggcg ccgcggtagg ccgagcggta
      241 catgtcctcg agcggttcta tcaccttctg gacggcgaag atctcggaga cgatgccctg
      301 ctccagctcg acgtgggcca cgagcgcgtc gacgtcgccc gactcgatgt cggtgcgctg
      361 gcgctcgagc agggacatgt attcgttaaa tttgtcgcgt tggcggacga gcagttccct
      421 gaagcgcttg agtacggcga cccgctcgtc cagctcttgc ctggacagct tcccgtcggg
      481 aggcatccgc gcgcctagcc ggctatgttg acgccggagc gccgctcgtc gcccgagccc
      541 tgggccttgg tggccgcgac ggcccaggcc gatgacagct cggccagcat cgccctgatc
      601 gcgcggaccc ggccgacgtc cttggcgatg ttcgcctcgg tcagctcgcg gttgaaatag
      661 acgtataggg agaacaggtc gcgggcgatg tcgccgccgg cctcgaagtc gaggctggcc
      721 atcagttcgg tgacgatgtc ctgaaccttg caaatcgcct ggttgacctt ctcgatgttg
      781 gcgggcttct tcttgaggtc cgtatcgagg aagtctaccg ccctgtcgca ttgcttgacc
      841 gcctcctcgt acagcatgac gacgagctgg cccgggccgg cggtgcgtat gcgggtttcc
      901 ttgtaggcag cgagcggatt ctgggtcatc tggatctccc cctcctctga tcttcttctt
      961 tatatatcgg aacgggaagg gctggcttga gcataaaatc attcgtctcc cagggtgccg
     1021 gtgtcgcgcc aggtttcgat aatcctgtag gcgtcgttga tgcgcgcgct cgtctcggtg
     1081 gccttgagct gggcctccgg cgacgaaccg tgcctgtccg ggtggtgttc cttgagcagc
     1141 cgcttgtagg cggccctgac ctggtcgaac ggagccccgt aggcgaggcc gagcgtcttg
     1201 taggcggcga cgagcttggc cggaggcccg gaggcggcgg ccggacgcgc ggcgcgttcc
     1261 tcggcgcgag cccgcgcctc gcgctcgcgg cgcaggcgtt cctgggactc gcggtcgtcg
     1321 ttcaggaacg cgtcgagctc gtccatagcg tcgtcgagga acgggtcccc ggaactcttg
     1381 gagaatttcc ccgcgaaggg atccgagaag gggcgcgacg agcccggatc cgattgaacc
     1441 caggacttga gcaggtcgcc gaaccgatcg aagagttgat ccatgctgga catacggact
     1501 atactctaca acaagccggt ccgagaggca agacgagcgg tacgacgagg agcagcatga
     1561 gcgggttcaa gccggtggcg gccgtctacg gcggcgcgaa tatcgatatc caggcgcgta
     1621 gccggggccc ctaccgggcc ggcgactcga atcccggcga ggcgtccatc acgcacggcg
     1681 gcgtcggccg caatatagcg gagaacctgg ccggcctagg cgtgccgacc gagctggtca
     1741 cggtcttcgg cggcgacgaa cagtcgtcgg gcctggccga gggttgtagg cgagtcggca
     1801 tcgaagtcag ctcgtcgctg ttcctgccgt cggagccatg ctcccgctac gtctgcctgc
     1861 tcgatcgcga cggtacgctg gtcggggcgg tagccgcgat ggattcgctc gaggctttcg
     1921 gccccgacca gctggcctcc aggttcgggc caggcgacgc ggccgaggtg gtcgtggtcg
     1981 acgccaacct accacccgaa accgtcgcgc tcgcggccgg ccgctggaag gacaagccgc
     2041 tgatcctcga taccgtctcg gtcgccaagg cccgcagagt cgcgccagtc gtcggcctgt
     2101 tcggtctcgt caagccgaac cgggccgagg cccgggtcct gctgtcgctc gacccctccg
     2161 agcgaccagt cgacccggag gccgaggcgg taggcctgac acgggggctc ctggcgctcg
     2221 gcgtgcgcga ggccttcgtc tcgctcggcg ccaggggctt cctctgggcc tcggcggctg
     2281 gagccggttt cgcgcgaccc ctggcgatgc cagtcgtcaa cgtgtccgga gcgggggacg
     2341 cggccaccgc ggccctggcc tgggccgctt cccaaggctt ggataccgaa accaaggcgc
     2401 gctacgccgt ggcggccgcg tccgtctgcg ctgcggacga gcgcgccgta gcggctggag
     2461 tgagcgccga tagactgtct gcattgatac gaggagtaac ctgtgaacgc attgcttgac
     2521 ctgagcgagg aagtccgcga cgcgatgggc cgaggcgccc ccgtcgtcgc cctggagtcg
     2581 accatcatat cgcacggcat gccctacccg aggaatgccg agacggccat cgccgtcgag
     2641 gccgaggtgc gagccgccgg cgccgtgccg gcgaccatcg cgatcatcgg cggccgcctg
     2701 aaggccggcc tgtccgagtc ggagataggc ctcctcggcg aacggggcca cgccgtagcc
     2761 aaggtcagcc gccgagacat aggtcgtatc gtcgcggcca aggcggacgg ggcgaccacg
     2821 gtggccggca cgatgatcgt ggccgccatg gccggtatca aggtgttcgc gaccggcggc
     2881 ataggcggcg tacaccgcgg cgccgagctg acgatggacg tgtccgccga cctcgacgag
     2941 ctcggccgca ccgatgtcgc cgtcgtctgc gccggcgcca agagcatcct ggatatcccg
     3001 atgacgctcg agtacctgga gaccaagggc gtgcccgtcg tcgtctatag gggcgacgaa
     3061 ttcccggcct tctacacgag ccgctctggc ctgcgcgccg agctacgcat ggactccgcg
     3121 gccgagatag ccgcgatgct caaggccaag tggtcgctcg gcctgtcggg cggcgccgtg
     3181 atagccaacc cgatcccgac cgagtaggag atgcggccgg aggtcataaa cgccgccatc
     3241 gagcgcgccc tcgccgaggc caaggccgcc ggcgtccgcg gcaaggacgt cacgccctac
     3301 atgttggcca aggttaagga tttgaccgga ggcgacagcc tcgaatcgaa catcgcgctc
     3361 gtcatgaaca atgccaggct cgccgccgcg ctggcgatgg agtacgctcg actatgacga
     3421 gggggcgccg cgcccggggc ctcgcctcgc tggcctgcct cgccgccctg gccgcgggag
     3481 cggtaggccc cgcggcggcc gcggacgtcg tgcgcttcgt caacctcggc ctgggcgacg
     3541 gcttggccaa cgcgagcgtc tcgtccatgg tccaggacgc cgccggcttc atctggatcg
     3601 gcacccaggg cgggctccat cgctgggacg ggctatcgtt caccctgtac gagaacgagc
     3661 ccttcgaccc ggagtcgctg ccgcacaacc tgatccagac cttgtttctg gacgaagatg
     3721 gcgaaacgtt atggatcggc acctacggcg gcctctcgcg tttcgacacg aggaccggcg
     3781 tcttctcgtc ctgggcgcac gacccggccg atcccggctc gctggcgaac gacgtggtcg
     3841 tctcgatagc cagggacgcc gagggacggc tatgggtcgg tacgctcggc gggctcgacc
     3901 gcatgcacgg cgactcgttc gtccattacg aggcgagtcc ggaggccgag ggcgccctgg
     3961 cctcgggcgt gatacgcgca ctgtaccggg actcccgagg ctggctctgg gtcggcacgt
     4021 cgggaggcgg cctgtatcgc tacctgcccg ggaccgactc cttcctgtcc gtacgctcgg
     4081 cctcgtccgg cctgccctcg gattacgtgt tctcgattcg tgaggatggc gagggccgcc
     4141 tctggctggc gctctggttc tatgggctgt cgcgctacga ccccgtcgga gggaccttcg
     4201 agaatcgaag gctcgaggac ggccgggcct acttcgtcaa cgccgacctc gacggtctgg
     4261 tctatgtcgg tacctggggc ggcggcctct tcgagctgag cttggacgac ggggcgatcc
     4321 gtcgattcac cagcgacggg gagcgccgat ggtcgctccc ccatgacacg atgtactcga
     4381 tgctcgtcga tcgggccgga gacgcctggg tcggtacgaa cggcggcggc ttctcgatgc
     4441 tattacacga cgaggccggc atgtcgctct acgaacacga cccgtccgac cccggctccc
     4501 tatcgtctgg caagagcatc tccgtgctcg aggaccggcg gggacgactc tgggtcggcg
     4561 cctataacgg cggcgtagac cggctcgatc ccggctcgtc gtccttcagg cgctatcgcc
     4621 atgatcccga tgacgagcgg agcctgccga acgacatcgt cgtcaatatc tacgaggatg
     4681 gccggggcgg gctctggttc ctgaccaatg acggcctggc caggtacgat gaggggcgcg
     4741 acgcgttcga gcgggtgcgc cgggatcgtt cctccaccga gggcgacgcc gtccgtatcc
     4801 tctatgatat ggttgaggag cccggtacgg gcagatactg gatagcaacc tacacctcgg
     4861 gcctcgagct ctgggacccg gtggacggca cggtggagcg ctatccgtcg gtgccgggcg
     4921 acccttccac cccgagcgac aaccttgtgt acgcgatcgc ctacgacggg cgcggccggc
     4981 tctgggccgc gacgaacgcc ggcctgtgcc gctacgaggg cgacgggcgc ttcgtccgct
     5041 acgaggcttc gaagacggac cgctccaagc taccgtcccg gtcgctacgc gacgtctacg
     5101 tagactcgct cgggaggctc tgggtatcga caagcggcgg cggcgtggcc aggtacgtcg
     5161 aggagagcga ctcctttgag cactggacca agcgcgacgg gctccagtcg aatatcgtac
     5221 acggcatcgt cgaggcggct gacggatcga tctgggcggt gtcgacctcc ggcctgtcgg
     5281 tgttcgacgc ggccggaggc cggtggcgcc cgttctccga ccagggccgc ctgcgctacg
     5341 gcgagttcac ctcgggccgc ttcgcctcgc gttccggcct cctgtacttc ggcgcggtca
     5401 acgcgctcta tgtcatcgat ccggccgcga tcgccggctc gccggacgcg ccgcctatca
     5461 ggttcacgag catcaagagc ctgaacgagg agctggacac cggcctgtcg ccgtggttcg
     5521 tcgacagagt caggctcggc tggagcgacc ctcatctgtc gttatccttc gcggccctcg
     5581 attacgccga tccgaccagg gtccagtacg cctataagct cgagggcttc gatgaggatt
     5641 gggtctattc gggcgcgcgc tcgtacgcca gctataccaa ccttcctggc ggcaggtcgt
     5701 attcgttcaa ggtgcgggcc gccacggcta tcggcgactg ggacggagag gcgaccgagc
     5761 tcttcatcga ggtcgacaag gcgccgtggc tacgctggtg ggccatcctt ctgtacctcg
     5821 tcgcggccgg cctcgtggcc ttcacggtca ggctatacgc cagccgggcc atgctcaaga
     5881 gtagggtagg cgaactgtcc gccctcaagg gccagctcga gagcgccaac gtcaagctcg
     5941 aggctctggc tgcccacgac ggcctgaccg gcctccataa tcgtagggcc ttcaacgacg
     6001 aactggccgc gcgctggcag gccgccgggg ctaccggtga accgctcgcg gcgctgatga
     6061 tagatatcga cttcttcaag ccctataacg accgttatgg ccatcaggct ggcgacgcct
     6121 gcctggcggc catagccgcg gccgtcaagg ccgccctcga ccgtccgcac gactcggcct
     6181 cccgctacgg cggcgaggaa ttcgtggccc tgctacccgg gacggacgcg gccggggcgg
     6241 ccagggtagc cgagaaggtc agggcggcca tcgaggccct cggcatcccg catggcggct
     6301 cgagcgtcgc ttctgcggtc acggcgagcg tcggctacgc gtccctgcgg ccggagcccg
     6361 gatcgcctcc gggccgcctc gtcgatcttg ccgacgaggc tctgtaccgc gccaagaacg
     6421 gcggacggaa ccgcgtctcg ccatagcggg ctgcgcaact tctagaagcg ggccgttccg
     6481 tgctatagtc gacccatggc atcgaaaggc tccgcttcaa agtacgacga atcgaagata
     6541 aagactctgt cgtcgctcga gcacatacgg ctacgcaccg gcatgtacat cgggcggctc
     6601 ggcaccggcg cgaatcccga cgacggcatc tacgtcctcc tcaaggaggt cgtggacaac
     6661 gccgtcgacg agttcatcat gggctgcggc gaccgcgtga ccgtgaccgt ccaggacggt
     6721 tcggtgtccg tgcgcgaccg cggccggggc atacccctgg gcaaggtgat cgagtgcgta
     6781 tccgtaatca acacgggcgc caagtattcc gacgacgtgt tccagttctc cgtaggcctg
     6841 aacggcgtcg gcaccaaggc cgtcaacgcc ctgtcgtcga gcttccgcgt cgtctcgtac
     6901 cgcgaggggc agttcttcga ggcctgtttc gagcgcggta cgctcatgtc caagcgcgag
     6961 ggccaggcca ggcgcgagcc gaacggcacc ctggtcgagt tcacccccga cgcccagatg
     7021 ttcggcgact acgcgttcaa catggaattc gtcgagaagc gcatgcgcca ctacgcctac
     7081 ctcaactccg gcctgaccct cgagctgaac ggccaggagt tcgccagcca gaacggcctc
     7141 ctcgacctcc tcaaggacga gacgggcgac gacgtactct acgaggcagg ctactggcgc
     7201 ggcgagcgaa tagagttctc gttcacgcat tcgaacaact acggcgagga gtacttcagc
     7261 ttcgtcaacg gccagttcac ctccgacggc ggcacccacc tgtcggcctt ccgcgagggc
     7321 ttcctcaagg ccgtcaacga gttcttccgc gcctcgtaca agggcgagga catccgcgag
     7381 gggcttgccg cggcggtcgc cgtcaagctg gccaatccca tgttcgagag ccagaccaag
     7441 aacaagctgg gtaacggcga tatcaagccc tggatcatcc aggaggtggt ccggggcgtc
     7501 gacgactacc tcaggcgcaa cccgacggcc gccaagcggc tcgaggagaa gatcgccgcc
     7561 aacgagaggc tccgcaccga gctcaacgtc gtcaagaagg aggcccgcga ggccgccaag
     7621 aagatagagc tcaagatacc caaactcaag gactgcaagc tccacctggg cgacggcccc
     7681 gacggcgacc gatcgacgat cttcctgacc gagggccagt cggccaccgg ttccatggtc
     7741 agctgccggg acgtgatgac ccaggccatc ttcagcctgc gaggcaaggt cgagaacatg
     7801 cacgcccgca agcgcgtggc tatctacaag aacgaggagc tgtacaacct gatgatggcc
     7861 ctcggcatcg agaacgacgc cggggggctg cgctacgcgc gcatcgtcat agcgacggac
     7921 gccgactacg acggcttcca tatcaggaat ctgctcctga ctttcttcct gaccttcttc
     7981 gaggagctcg tcaccgccgg acgcgttttc atcctcgaga cgccgatctt ccgcgttcgc
     8041 accaagaagg agacccgcta ctgctacagc cccaaggaac gggatgaggc ggtcggagcc
     8101 ctcggttccg gctcggaggt gaccaggttc aagggcctcg gcgagatcag cccgagcgag
     8161 ttcggccagt tcataggcga cgatatacgg ctcctgtcgg tcgacgtgca gtcgctcaag
     8221 agcgtacccg aggtgctcgc cttctacatg ggcaagaaca cccccgagcg ccgcgacttc
     8281 atcatgaaga acctcaagaa cgacctgtag gaatcatatg gcatacgtga agaagctgtt
     8341 caacgataat ttcctctact acgcgagcta cgtgatcaag gaccgcgcca tacccgagct
     8401 cgaggacggg ctcaagccgg tccagcgacg catcctccac acgctgttcg aggcggacga
     8461 cggtaagttc cacaaggtgg ccaacatcgt cggctcgtgc atgaagtacc atccgcacgg
     8521 cgacgcctcc atcggcgacg ccctcgtcgt gctggccaac aaggacctgt tcatagacaa
     8581 gcagggcaac tacggcaaca tcctgaccgg cgacgaggcg tcggcggcca ggtacatcga
     8641 gtgccgcgcg tcggccttcg ccaaggatgt cttctacaat cccaagatca ccgagtacgt
     8701 cgagagctac gacggtcgca acaaggagcc cgtcgtcttc ccggccaaga tacccgtcat
     8761 cgccatcacc ggcggcgagg gcatcgcggt cggcatgagc acgaagatcc tgccgcacaa
     8821 ccccgtcgag gtactcgccg ccgagatagc ctgcctgcgc ggtgaggcat tcaaactgta
     8881 ccccgacttc ccgaccggcg gtatcgtcga cgccggcgag taccaggacg gactcggccg
     8941 ggtgcgcgta cgctcgaccc tggacacctc cgaccccaag cgcatcgtca tccgcgacct
     9001 gcccttcggc gtgactacgg agagcctgat cgaaagcgtc gaggccgcgg ccaaggccgg
     9061 ccggctcaag atagcctcga tcagcgactt caccacggac aaggtcgaga tcgaggtcaa
     9121 gctggcccga ggcgtctact cgcgcgagac cgtcgacgcg ttgtacgcct ttacccagtg
     9181 cgagcagtcc atatcgtgca acttcctcgt catcaaggac ggccagccct gcctgatgac
     9241 cgtcaccgag gtcatacgcc accacgccaa gcgcctcctg gcaatcttga agaaggagct
     9301 cgagctccag gtcaaggaac tgctcgacga gctccacgcc cgcaccctcg agcgtatctt
     9361 catcgaggag cgcgtctaca aggccatcga gaccaagaag acccagggcg ccgtcgacaa
     9421 ggccgtgctc gacggcctcg ccccgttcgc cgacgagatc aaacgcgaag tgaccgcgga
     9481 ggacgtcgaa cggctcctac gcattccgat acgccgcatc tcgctgtacg acatcgagaa
     9541 ggccagggct gacatggagc gcatcaaggc cgccctcaag gacgcgcgcc accacctggc
     9601 ccacctgacc gagtacggca tcgccttcct cgagggcctc cgtaagcggc tcgggccggc
     9661 ctgggagcgc aagacgcggg tcgcctcgtt cgaccgagtc gacgccaagg aggtggccag
     9721 gcgcgacata gccctgcgct acgaccccaa gaccggctat ctcggcacca acgtctccgg
     9781 cggcgagtac cgcttcgacg tatcgcccta cgataagatc ctcgtcatcc gtcgttcggg
     9841 catctactcc gtcgtgccgg taccggagcg tctcttcgtc gacaccgaac tcctgtactg
     9901 cggcatcgcc gacaaggacg agctggccga cacggtcttc accttcgtat atgccgatcc
     9961 caagaacggc tcgccctata tcaagcgctg caagatcgag gcgtggatca tgaaccgcga
    10021 ctaccagctc ggcaaagagg gcggctcgct cctgtacttc acgaacgagc ccaagaaggc
    10081 cgtcgtcctt cgctacgcac ccaagccgcg cttcaagatc aacgaggagc gtttcaggat
    10141 agagagcttc ccggtcaagg ggctcaaggc cctcggcgtg cgcatgtcca cgcggcaggc
    10201 cctgtccgcc gcggccgaag gcgagggcgg gctgttcgac gaggggggct cgaagctatc
    10261 aagccgaacc aacgaggcag gagcagctac gtcggctcag tcgagcccaa ggtcgactat
    10321 ggggccgtcc accaaggcgg cgggcaaagc cgccgccgga gccgctccga agggcgccgc
    10381 tcggaaggtc gctccggcca agccggccgc cggcaagtct ccggtcgcca agaaggctac
    10441 ggtatcgaag gctacggcta ccaagaagcc ggccgccacc aagaagccgg caagcgctca
    10501 gaagccggca accgctcaga agccggccac caccaagaag ccggccgcaa aggacgacga
    10561 ccagccccat ggtggcctcc tcgagcgggc ggcagccaag aagaagccga ccaagggcta
    10621 gggccgctcg tcgatcaccc tgaaacccgc tatctcaccg gctatctccg agatcagccg
    10681 cccgttggcc atggtatgct ccagtaccga ggcaatggcc ccggcggccc ccctgacgcg
    10741 ttcggccatc gcgccgacct cggccgagac cgaggcgttc gcctcggtga gtcgagcggc
    10801 ggtgtcggcc agggcggccg atttggctcc gagttctccg gagtagtccc tgacccgtat
    10861 gccatccgcc tgcatcgccg acatagcggt agatatttgg gaagagccgg cggcctgctc
    10921 gcgcatcgcg ttctccatct cctccatgag gtccgatact tggcggacgc cgccctgcac
    10981 cgactcgaac gcctcggtgg ccgagcctga ggagccgaca gcctcgtcta tcgagagctt
    11041 gacctgatcc agcgcggccg caatctcacg ggatcgctca gccgattcct ccgatagtcg
    11101 cctgatctcg tcggccacca ccgagaagcc tttgcccgcg tcgccagcgt gcgcagcctc
    11161 gatcgccgcg ttcatggcca ggaggttcgt gctcttggct atgccggcga tcacctcgac
    11221 ggcgtcgagc atcgcgccgg agcgctcggc tatcgcgctg accgcgctcg tgacctcctc
    11281 gatcctcgag cggccgtcgt cc
//
LOCUS       DAOPHV010000016        11206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_7039_length_11206_cov_3.889427, whole genome shotgun sequence.
ACCESSION   DAOPHV010000016 DAOPHV010000000
VERSION     DAOPHV010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 11206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11206
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7039_length_11206_cov_3.889427"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PLQ29_00900"
     CDS             <1..730
                     /locus_tag="PLQ29_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85227.1"
                     /translation="AMAAGFLLIQIWMMYDLQVLSALFDNLALSLEPPILGFFVIYCV
                     YRPLRHAVLNGLERETPEAGAAPARGASPIQARTRWSLARWAAGIVVMASVLAAWNLV
                     AYGRIGRFVDLAPLSLALFLPLGQLLAGPGPNGLRSAFAALSTDDPSALRSARRIFAF
                     NAGSFAVAAALAFSLGAAYFLEAIGDRMRYGPTLALAELGLLQSAVLALVLALPPLAA
                     ADRALAAADRALAATEPNDRGGRA"
     gene            792..2330
                     /locus_tag="PLQ29_00905"
     CDS             792..2330
                     /locus_tag="PLQ29_00905"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HPG85228.1"
                     /translation="MSERQGARQTKASGGRGAGVISARSFVASLAVLAALMLAAGVLT
                     RLVPAGSYERSAGADGALVVVPGSYVETARPDYPAWRWLTAPFEVLASEDAALAIVII
                     AFIAVVGGAFAAMREAGVLAESVRLLAERFKARRMALLAVLCLFFMAIGSFMGVFEEV
                     VPLVPIAIALSLSFGWDVFAGLGMSILTVGFGFSSAVANPFSVGTAQRLAGVPMLSGS
                     GFRLVVFACVYALYLTFMVGYVRRLERKGLGSTMDRADGSPVSASAYGSGSAGSPGSA
                     GDTGRAGPDQGATSPAAPFVSRGVRFFGWSALVLAILVLLSSVTRVGADYVLPAIALG
                     FLVIGVGAALAAGVRLGAVARSFGTGALGVLPAGLLILMALSVKRIVVAGGIMDTALY
                     AASGLMERASGYGAAALMYGVVLVMNFFIGSASAKAFLLIPVLAPLADLTGLSRQLAV
                     QAFVFGDGFSNMLYPTNAVLLISLGLAGMSWGAWFKKTWRLQLATLALTMGLLMVAVA
                     IGYA"
     gene            2352..3650
                     /locus_tag="PLQ29_00910"
     CDS             2352..3650
                     /locus_tag="PLQ29_00910"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPG85229.1"
                     /translation="MKRLFAVPALAAALAAITFSAFAQAPAVGPASWLGRWDGRLEIG
                     EGGMPFSVRIVEAGALLDLPGEELYGYPSASAEASADGLRLAFRFGGGLLTLTGGLEG
                     GSVSGAFSQGAAGGAFSMERSAAPAAPGTARVVRGADGSALYGTLLEPTGTGRPPLVI
                     LHAGLGAADRDGNNYASRGRHDALRALGEALAPRGVASYRYDKRGSGESAWLVPSEAD
                     QSYEAWIADLAAMVAEFGRSGRYSGVWLLGLNDGAAIAAAAANSLRAAGSPVAGLVVA
                     CASADGVRDAYAKAVARAPEGLRAEGEALVASLSAGSIVDSVSSYYAEAFRPSLQPYL
                     IEAFARDLETELAAYAGPCLLVQGNMDLQATLADLLALRATRPDALVAMPLRMNHVLK
                     DVSQDVEDNRAAFADPSYPVSAELVDAITGFIEGTFVGIP"
     gene            3890..4924
                     /locus_tag="PLQ29_00915"
     CDS             3890..4924
                     /locus_tag="PLQ29_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85230.1"
                     /translation="MQDRQKLVGAMFRAPGFAAVAAATVTLMYRAGLMAWPTVPLLAA
                     IKLPLLAGLVVGLALEAKAAFVISPGEAPSVGSRGVAAGAAVGDIADAEFADAAAVLA
                     SALIGYLAIARLGLNAVLASSLLGLAGALLLPKRAVAIYCGSFAGMAAPAAFETMPSV
                     ALSGVIAGVFYVSCKPCFPGVGGRLGAAAYAGSLGSSLLLGARLTGDELPGLGLGLGI
                     LAYAILGSALTWTVSVRLGRGAVMASAFWGAVAGAVIPALHGPVAGSLLDAAFFCGSF
                     AGMSSAARLRNEAEAGVAGAFCGLSFILALPWLGGAGGKLGTLAFGSTLAYLGLRETV
                     RRALSRLRKT"
     gene            5080..5877
                     /locus_tag="PLQ29_00920"
     CDS             5080..5877
                     /locus_tag="PLQ29_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG85231.1"
                     /translation="MIAVENLRKTYRVKKRPAGLVAAAAALVRPRYDTVEALRGVSFH
                     VSQGEIVGYIGPNGAGKSTTIKVMSGILVPDSGSCVIKGRVPWKERKAHVAGIGVVFG
                     QRCQLWWDVPVIDSLDLLRDIYRVDEAAYRRNLDELVSALDIGGVLGQTARSLSLGQR
                     MRCEIAASLVHAPDILFLDEPTIGLDAVSKLAVRGFIARANRARGLTVMLTTHDMHDI
                     EALASRVILIGKGEILHDGSFGSLVAGRGDGGVESAVADLYGEMGIA"
     gene            5877..6683
                     /locus_tag="PLQ29_00925"
     CDS             5877..6683
                     /locus_tag="PLQ29_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG85232.1"
                     /translation="MGELPAYAGLFRMRLVRGLSYRAAAIASSVTQFFWGFMLISVLR
                     AFARSGGSPLDDGQIASYIWLQQASLVLGAIWFRDGELVRLICSGDAAYELCRPVDLY
                     RFWFARLAGGRLAATALRCLPILAVASFLPEPWRLCLPGSPAVLACFALAMALATVMV
                     VAVSMFSYALTMVTLNTQAGFLFVAPVFELCAGLLVPLPLMPEGVRRVLEVLPFRYAA
                     DLPFRIWMGSIGLAELPGLLAAEIAWLAVIVAGGRLALGAALRRAEAVGG"
     gene            6680..7504
                     /locus_tag="PLQ29_00930"
     CDS             6680..7504
                     /locus_tag="PLQ29_00930"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HPG85233.1"
                     /translation="MRAARLYLRCAGVAVQGQLSRRYNLVLLAFGQLSIAVSALIGVA
                     LLFSRFGSLAGYSFSEVLLCFGVTQTAFSLAEVVARGFDSFSQVLAQGEFDRMLVRPR
                     GSMFQVLASRMELARLGRVSVGLACLASALAALVPGWAADAIAVGQAGLLVLRLATLA
                     LMTAGGMAIFSGIFILGASLAFITTDSLEIVNVLSDGGRETAQYPLTIYERAMRRFFT
                     FVVPFGCVNYLPLEFVLGRPGSAPWSACFPLVGFAFLAASTAVWNASARRYVSTGS"
     gene            7702..8469
                     /locus_tag="PLQ29_00935"
     CDS             7702..8469
                     /locus_tag="PLQ29_00935"
                     /inference="COORDINATES: protein motif:HMM:NF009920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HPG85234.1"
                     /translation="MNDIRYRAFHQFVAFVPDEEIGLRGAKLLDLGRFSPDYAYTIDC
                     CELGEVVYETFNAGSAFIEIEGVTAHPMSAKGVLVNPILVAQDLIALFDRSETPERTE
                     GKEGYVWFNRISGGQSSVSLQASIRDHDLAGYDDRKRRLLAAVEEVGRRYPRARLSIR
                     IDDVYANIANHADRSHPAVSAIYGAMAELGIEPRTVAMRGGTDGSALSARGLVTPNYF
                     TGAHLFHSRYEFLPMRSFVASYRLTRRLIEDAGTRLR"
     gene            8576..10738
                     /locus_tag="PLQ29_00940"
     CDS             8576..10738
                     /locus_tag="PLQ29_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85235.1"
                     /translation="MKNLVVALAICSALLAALVACRSPLHGTNDTTDVSIRIGGVLPP
                     TLSDSATRHGARVIVPAAVQLRVTADWERGGQLIQTSQVAGGQATIRRLPVGVAISLY
                     LEALDSSGTVLSVGTLALAPLGEGLNAAPAVTLKPSPAMATDDLGAMSAGMSSARSVG
                     AQGVRYYTFTAGDGLSGDFQVVFDTGKARWVWLKAYDEDWNELPAICSDASQGWVVVR
                     LAETQTVNLAVSNSVGSPGYPAGAIDGAIVVRPAKFCTADALELGDGSLSAPLTIESI
                     GSMCDSGDSLFVRGGLYDASAQVTSSLGDLYVYGGFSPDWKTRDARLYETRIGSSGEV
                     PDLVRVAGGGLLDGIRITAIPLSGSVMNTCGLILKPQSALVINDCRIYGGTNYEAISS
                     QSIGSVAVNVTSETGGTIEMASCELYAGGANNLDEFPASTYALSVSSSVVYVHDCDID
                     GGFAQSPGGASTHGVWVTNAYVILARSRVWGGIAKSSSVDYDASAAGVFAEYGTRTVA
                     IVNSLVSGGYSSNSAAGGREYLRGVETYQQPIRICGSVIDSGFCPEPVETYSYVSATS
                     TEESGTVQIESSIVITSNATAFGDLFYDAEMPNSATTCYGCLAAHPGEFSYGGSDSAF
                     PEPIAGNEDLPNLALGGFFTAFSNAYTGSYPSDYERWLGGSWRVSPGYSAYIGGGNLP
                     YSGGSVAGFSVADYPALVLDLAGKARPTSGSWGRGAYE"
     gene            complement(10928..>11206)
                     /locus_tag="PLQ29_00945"
     CDS             complement(10928..>11206)
                     /locus_tag="PLQ29_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85236.1"
                     /translation="MMLSARASSGQAAALGAYVASLAFEFDDERSAVIFAPICRLFLY
                     VAATALWPERSATVLDAARWNASGRVVRADGIPLDAEDLAAFVTLGAR"
BASE COUNT         1565 a         3868 c         3728 g         2045 t
ORIGIN      
        1 cgcgatggcc gccggcttcc tgctcatcca gatatggatg atgtacgatc ttcaggtcct
       61 gtccgctctg ttcgacaacc tggccctgag cctcgagccg cctatcctgg gtttcttcgt
      121 gatctactgc gtgtaccggc cgctacgcca cgcggtactg aacggcctcg aacgcgaaac
      181 gcccgaggcc ggcgcggcgc ccgcacgggg cgcgtcgccg attcaagccc ggacgcggtg
      241 gagcctcgca cgctgggcgg cggggatcgt cgtcatggcc tctgtcctag cggcctggaa
      301 cctcgtggcc tatggcagga taggccgctt cgtcgatctg gcgcctctgt ccctggcctt
      361 atttctaccg ctcggccagc tgctcgcggg gccaggcccg aacggtctgc gctcggcctt
      421 cgcggccctg agcacggacg acccctccgc cctgcgttcg gcgcgccgca tcttcgcttt
      481 caacgccgga tccttcgccg tcgccgcggc gctcgcgttc tcgctcgggg ccgcgtattt
      541 cctcgaggcc ataggcgacc gcatgcgcta cggccccacg ctggccttgg ccgagctcgg
      601 cctgctccag tcggccgtcc tggccctcgt cctggccctg ccgcctctgg ccgccgccga
      661 ccgagcgctg gccgccgccg accgagcgct ggcggctacc gagccgaacg atcgcggcgg
      721 ccgcgcgtag cgccgctttt cgcttgcggc ctccgccagg gccgtgtacc ctggatcgcg
      781 gaggcgcgag catgagtgaa cgacaaggcg ctagacagac gaaggcttcg ggcggccggg
      841 gcgccggggt catatcggca cgctcgttcg tcgcgtcgct cgcggtgctc gcggcgctga
      901 tgctggctgc cggtgtcctg acgcggctcg tgccggccgg ttcgtacgaa cgctcggccg
      961 gggccgacgg cgccctcgtc gtggtgccgg gcagctacgt cgagacggct aggccggact
     1021 acccggcctg gcgctggctc acggccccct tcgaggtgct cgcctccgag gacgcggccc
     1081 tggcgatcgt gatcatcgcg ttcatcgcgg tggtcggcgg cgccttcgcc gccatgcgag
     1141 aggccggcgt gctcgccgag agcgtccgcc tcctcgcgga gcgcttcaag gccaggcgga
     1201 tggccctcct ggccgtcctg tgcctgttct tcatggccat aggcagcttc atgggcgtct
     1261 tcgaggaggt cgtgcccctc gtgcccatcg cgatagccct gtccctgtcc ttcggctggg
     1321 acgtcttcgc gggcctcggc atgagcatcc tgacggtcgg cttcggcttc tcgtcggccg
     1381 tggccaatcc cttctccgtc ggcaccgccc aacgcctcgc cggggtaccg atgctgtcgg
     1441 gatcgggctt caggctcgtc gtcttcgcct gcgtctacgc gctgtacctg accttcatgg
     1501 tcggctacgt gaggcgactc gagcggaaag gcctagggtc gacgatggac cgtgctgacg
     1561 gctcgccggt ctccgcctcc gcctacggct ccggctccgc cggttcgccc ggctcggccg
     1621 gggataccgg ccgagccggc ccggaccaag gcgctacctc tccggccgcc cccttcgtct
     1681 cgcgcggggt gcgcttcttc ggctggtctg ccctcgtcct cgctatcctc gtgctcctgt
     1741 cgtccgttac ccgcgtcggc gccgactacg tgctgccggc gatagcgctc ggcttcctgg
     1801 tcatcggggt cggcgccgcc ctggccgccg gcgtaaggct cggcgcggtg gcgcgctcgt
     1861 tcgggaccgg cgccctcggc gtgctgccgg ccgggctcct gattctcatg gcgctgtcgg
     1921 tcaagcgcat cgtcgtcgcc ggaggcatca tggacacggc cctctacgcc gcgagcggtc
     1981 tcatggaacg ggcctcgggc tacggcgccg cggccctcat gtacggcgtc gtgctcgtca
     2041 tgaacttctt cataggcagc gccagcgcca aggctttcct gctcattccc gtgctggcac
     2101 cgctcgccga cctgaccggc ctgtcgaggc agctggcggt gcaggccttc gtcttcggcg
     2161 acggcttctc gaacatgctg tacccgacca acgcggtgct gctcatctcg ctcggtctcg
     2221 ccggcatgtc gtggggcgcc tggttcaaga agacctggag gctacagctc gcgaccctcg
     2281 ccctgacgat ggggctcctg atggtcgccg tggccatagg ctacgcctag cgccttcgct
     2341 atactgccgc catgaagcga ctcttcgcgg tccccgcgct cgccgccgcg ctcgcggcca
     2401 taaccttctc ggccttcgcg caggctcccg ccgtcggccc cgcgtcctgg cttggccgct
     2461 gggacggccg cctcgagatt ggtgagggcg gcatgccctt ctcggtgcgt atcgtcgagg
     2521 ccggcgccct cctggacctg cccggcgagg agctgtacgg ctacccgtcg gcgtcggccg
     2581 aggcctcggc cgacggcctc cggctggcgt tccgtttcgg cggcggcctc cttacgctga
     2641 ccggcggtct cgagggcggt tcggtatcgg gcgccttctc gcagggggcg gccggcggcg
     2701 ccttctcgat ggagcgctcc gcggctccgg cggccccggg aacggctcgc gtcgttcggg
     2761 gcgccgacgg atcggccctg tacggcaccc tgctcgagcc gaccggaacc gggcggccgc
     2821 ccctcgtgat cctgcacgct ggcctcggcg ccgccgaccg cgacggcaac aactacgcct
     2881 cgcgcggccg gcacgacgcc ctccgggcgc tcggcgaagc gctcgcgcct cgcggcgtcg
     2941 ccagctaccg ctacgacaag cggggctcgg gcgagtccgc ctggctcgtg ccgagcgagg
     3001 ccgaccagag ctacgaggcc tggatagccg acctggccgc gatggtcgcg gaattcggcc
     3061 ggtcgggccg ttactcgggc gtgtggctcc tgggcctcaa tgacggcgcg gctatcgcgg
     3121 cggcggccgc gaactcgctc cgggcggccg gctctcccgt agcgggactc gtcgtcgcct
     3181 gcgcctcggc cgacggcgtc cgcgacgcct acgccaaggc cgtagcccgg gccccggagg
     3241 ggctgcgggc ggagggcgag gcgctcgtcg cctcgctgtc ggcggggtcc atcgtcgact
     3301 ccgtctcctc gtattacgcc gaagccttcc gtccgagcct ccagccctac ctgatcgagg
     3361 ccttcgcgcg cgacctggag acggagctcg ccgcgtacgc ggggccgtgc ctgctagtgc
     3421 agggcaatat ggacttgcag gcgacgctcg ccgacctcct ggcgctcagg gcgaccaggc
     3481 ccgacgcgct cgtcgctatg ccgctacgca tgaaccacgt gctcaaggac gtgtcccagg
     3541 acgtcgagga caaccgtgcc gctttcgccg acccgtcgta ccccgtgtca gccgagctgg
     3601 tcgacgccat aaccgggttt atcgaaggca ccttcgtcgg cataccgtaa tcccgcgcga
     3661 cgctgagtac gcgatgcctc gcggaaagac gtaagggcac caagacgcaa cgatcacgaa
     3721 gcgaaggacc ctggaggcga aggcgttgaa caggatggac acgataaagc gatgaagcct
     3781 tggggcttat ccgcttgagc tgttcttcat cctgtcatca tgttcgaagt atcctgatgg
     3841 cggcgagtcg tttgcccgat cggggggcgg cgggtatact ggatccatca tgcaagatcg
     3901 tcagaagttg gtcggggcca tgttccgggc gccgggtttc gcggccgtcg cggcggcgac
     3961 cgtcacgctg atgtaccggg ccggcttgat ggcctggccg accgttccgc tgctggccgc
     4021 catcaagctg ccgctcctcg cggggctcgt cgtcggcctc gcgctcgagg cgaaggcagc
     4081 cttcgtcata tcgcccgggg aagcgccatc cgtcggctca cgaggcgtcg ccgcgggagc
     4141 ggccgtcggc gatatcgccg acgccgaatt cgccgacgcc gccgccgtgc tcgcgtcggc
     4201 cctgatcggc tacctggcga tagcgaggct ggggctcaac gccgtgctcg cctcgtcgct
     4261 cctgggctta gctggcgcgc tgctactgcc gaaacgggcg gtcgcgatct actgcggcag
     4321 cttcgccggc atggccgcgc cggccgcctt cgagacgatg ccgtccgtgg cgctgtccgg
     4381 cgtgatcgcc ggcgtcttct acgtgtcatg caagccgtgc ttccccgggg tgggcggccg
     4441 actcggcgcg gcggcctacg cgggcagcct cggctcgtcg ctcctcctgg gcgcacggct
     4501 caccggagac gagctgcccg ggctcgggct ggggctgggc atcctggcct acgcgatcct
     4561 tggatcggcg ctgacttgga ccgtctcggt gcggctcggc cggggcgcgg tgatggcgtc
     4621 ggccttctgg ggcgccgtgg cgggggccgt cattccggcg ctgcacggcc cggtcgccgg
     4681 cagcctgctg gacgcggcgt tcttctgcgg cagcttcgcc ggtatgagct cggcggcccg
     4741 gctacgcaac gaggcggagg ccggcgtcgc cggcgccttc tgcggcctgt cgttcatcct
     4801 ggcgttaccc tggcttggag gggccggcgg caagctcggc acgctggcct tcggctccac
     4861 gctcgcctac ctggggctca gggaaacggt acggcgcgcg ctctctcgcc tgcgaaagac
     4921 ctaatcatgt catcatattc gtagcagcct agttttcctc ttctccgcgt cctttgcgcc
     4981 ttcggggctt cgcgcgaagc atcgtaatag caatccggcg cgaagggttc ttcctacgca
     5041 cgcccggttc ctgtattgtg tccgcttcgg ggaaaaacca tgatagccgt cgagaacctc
     5101 aggaaaacgt acagggtcaa gaagcgcccg gccgggctcg tcgcggccgc ggcggccctc
     5161 gtgcgcccgc gctacgatac ggtggaagcc ctgcgcggcg tatccttcca cgtgagccaa
     5221 ggcgagatag tcggctacat aggaccgaac ggcgccggca agtccacgac catcaaggtg
     5281 atgagcggca tactcgtacc cgactccggc tcctgcgtca taaagggccg cgtgccctgg
     5341 aaggagcgca aggcccacgt cgcgggcata ggcgtcgtgt tcggccagcg ctgccagctc
     5401 tggtgggacg tgccggtcat agactcgctc gacctcctgc gtgatatcta ccgcgtcgac
     5461 gaggccgcct accggcgcaa cctcgacgag ctcgtgtcgg ccctggacat aggcggcgtg
     5521 ctcgggcaga cggcgcgatc cctgagcctc ggccagcgca tgcgctgcga gatcgcggcc
     5581 agcctcgtgc acgcgccgga catcctgttc ctcgacgagc cgaccatagg cctggacgcc
     5641 gtgtcgaagc tggccgtgcg cggcttcata gcgcgcgcga accgagcgcg cgggctcacg
     5701 gtgatgctca cgacccacga catgcatgac atagaggctc tcgcgtcgcg cgtcatcctg
     5761 ataggcaagg gcgagatact ccacgacggc agcttcggct cgctcgtcgc ggggcggggc
     5821 gacggcggcg tggagtcggc cgtagccgat ctctacggcg agatggggat agcgtgatgg
     5881 gcgagctccc ggcctacgcg ggcctcttcc gcatgcgcct cgtgcgcgga ctgtcctaca
     5941 gggccgcggc gatagccagc tcggtaaccc agttcttctg gggcttcatg ctcataagcg
     6001 tgctcagggc cttcgcccgt tcgggcggct cgcccctgga cgacggccag atagcgtcgt
     6061 acatctggct acagcaggcc tccctcgtgc tcggcgcaat atggttcagg gacggagagc
     6121 tcgtgcggct catatgctct ggagacgcgg cctacgagct gtgccgcccc gtcgacctct
     6181 accgattctg gttcgcgcgg ctggccgggg gacgcctcgc cgccacggcc ctgcgttgcc
     6241 tgcccatact ggcggtggcg tccttcctgc cggagccgtg gcgcctctgc ctacccggga
     6301 gcccggcggt cctcgcctgc ttcgcgctcg ccatggccct ggccacggtc atggtcgtcg
     6361 ccgtgtcgat gttctcgtac gccctgacca tggtcaccct gaatacccag gccggcttcc
     6421 tcttcgtggc gcccgtgttc gagctgtgcg ccgggctcct cgtccccttg cccctcatgc
     6481 ccgaaggcgt ccgccgcgtc ctcgaggtct tgccctttcg ctacgcggcc gacctgccgt
     6541 tcaggatctg gatgggctcg ataggcctcg cagagctacc ggggctcctc gcggcggaga
     6601 tcgcgtggct cgcggtcatc gtggccgggg ggcggctcgc gcttggcgcg gccctaaggc
     6661 gcgccgaggc ggtaggcgga tgagggccgc acggctctac ctgcgttgcg ccggcgtcgc
     6721 ggtacagggt cagctcagcc gacgatacaa cctcgtcctc ctggccttcg gccagctatc
     6781 tatagccgta tcggccctga tcggcgttgc gctgctcttc tcgcgcttcg gctccctcgc
     6841 gggctatagc ttctccgagg tgctgctctg cttcggggtg acccagacgg cgttctccct
     6901 cgccgaggtg gtagcgcggg gcttcgactc gttctcgcag gtactggccc agggagagtt
     6961 cgaccgcatg ctcgtcaggc cgcgcggctc catgttccag gtgctggcat cgaggatgga
     7021 gctggcccgc ctcggccgcg tgtccgtcgg cctggcctgc ctggcctcgg cgctggccgc
     7081 acttgtgccc ggatgggccg cagacgccat cgcggtagga caggcgggcc tcctcgtcct
     7141 gaggctcgcg acgctggctc tcatgacggc cggaggcatg gccatcttct cgggcatatt
     7201 catcctcggc gcgtcgctcg ccttcatcac gacggactcc ctcgagatag tcaacgtcct
     7261 gtccgacggt ggccgcgaga ccgcccagta cccgctcacg atatacgagc gcgcgatgcg
     7321 gcgcttcttc accttcgtgg tacccttcgg gtgcgtgaac tacctgccgc tcgagttcgt
     7381 cctcggccgg ccgggctcgg ctccctggtc cgcatgcttc ccgctcgtgg gcttcgcctt
     7441 cctcgccgcc tccacggccg tctggaacgc cagcgcccgg cgctacgtct ccacgggctc
     7501 gtgagcggcc ccgagcgggg gcgttaccgc gatagaggcg aagacagcga acaagatgat
     7561 catgatggga tggacacgat aagacggaaa agttatggac ttacactaag ccaccgagac
     7621 acggatgatc cgtgcccccg tgacttggtg tacgccagta ctagaatcct caagtcaagc
     7681 caataaggga caacgaacgc tatgaatgat atccgctata gagcttttca tcagttcgtc
     7741 gccttcgtgc cggacgagga gataggcctc aggggcgcca agctcctgga tctggggcga
     7801 ttctcgcccg actacgcgta tacgatcgac tgttgcgagc tcggcgaggt ggtctacgag
     7861 acctttaacg cggggagcgc cttcatcgag atcgagggcg tgacggccca tccgatgtcg
     7921 gccaagggcg tcttggtcaa ccctatcctg gtcgcccagg acctcatcgc cctgttcgac
     7981 aggagcgaga cgcccgagcg taccgagggc aaggagggct acgtctggtt caaccggatc
     8041 tccggcggcc agtcgagcgt ctcgctgcag gcgtcgatac gcgaccacga cctggccggc
     8101 tacgatgaca ggaagcgacg gctcctggcg gccgtcgagg aggtcggtcg gcgctacccg
     8161 agggcgcggc tatcgatccg catcgacgac gtgtacgcca acatcgcgaa ccacgccgac
     8221 cgctcccatc cggcggtctc cgcgatctac ggggcgatgg ccgagctcgg catcgagccg
     8281 aggaccgtgg cgatgcgcgg cggcaccgac ggctcggccc tgtcggcgcg cgggctcgtc
     8341 accccgaact acttcaccgg ggctcacctg ttccattcgc gctacgagtt cctaccgatg
     8401 cgttccttcg tcgcgtccta tcgcctgacg cgcaggctga tcgaggacgc aggtacgagg
     8461 cttcgctgag gcccaacgag ccgccgtggt ttttcgacac tcagtgatag aatagtcatc
     8521 attcaatacg attactttaa ggcttcatca tacttctcgt acagggagtt gagccatgaa
     8581 gaatttggtc gtagcgctgg ctatctgttc cgccttgcta gccgccctcg tcgcctgtcg
     8641 ttcgccgctc catggaacga acgatacgac cgacgtgtcg atacggatag gcggcgtcct
     8701 gcctccgacg ctatcggact cggcgacgcg ccatggcgcg cgggtgatcg tcccggcggc
     8761 cgtccagctt cgggttacgg ccgattggga gcgcggcggc cagctcatcc agacctcgca
     8821 ggtggccgga ggccaggcca cgatacggcg cctgcccgtc ggcgtcgcca tatccttgta
     8881 cctcgaggcg ctcgattcgt ccggtaccgt gctgtccgtc ggcacgctcg ccctggcgcc
     8941 gctcggcgaa ggcctcaacg cagcgccggc cgtcactctc aagccgtcgc cggccatggc
     9001 gaccgacgac ctcggcgcga tgagcgccgg catgtcgagc gcgcgcagcg tgggcgcgca
     9061 aggggttcgc tactatacct ttaccgcggg tgacggcctg tcgggcgatt tccaggtcgt
     9121 cttcgatacc ggcaaggcgc gctgggtctg gctcaaggcc tatgacgagg actggaacga
     9181 gctgccggcg atttgctccg acgcgtcgca gggctgggtc gtcgtacgcc tcgcggagac
     9241 gcagacggtg aacctggccg tgagcaacag cgtcggatcg ccagggtatc cggccggggc
     9301 catcgatggc gctatcgtcg tccggccggc caagttctgc acggccgacg ccctggagct
     9361 tggcgacggc tcgctctccg ctccgctgac gatagagtcg atcggatcca tgtgcgattc
     9421 cggcgacagc ctgttcgtgc gcggcggtct gtacgacgcc tccgcgcagg tcactagttc
     9481 gctcggcgat ctctacgtct acggcggctt ctcgcccgac tggaagactc gcgacgcgag
     9541 gctgtacgag acgcgcatcg ggtcttctgg cgaggtgcct gacctggtac gagtggccgg
     9601 gggcggcctg ctcgacggta tccgtattac tgccattcct ttatcgggaa gcgtgatgaa
     9661 tacctgcggc ctcattctca agccgcagag cgcgctggtc atcaacgatt gccgtatcta
     9721 cggcggcacg aactatgagg ccatctcgtc gcagagtata ggaagcgttg ccgtgaacgt
     9781 cacctcggag acgggcggca cgatcgagat ggcgtcgtgc gagctctatg ccggcggcgc
     9841 caacaacctc gatgaattcc ccgcttcgac ctatgcgctc agcgtttcca gtagcgtcgt
     9901 atacgtgcat gactgcgata tagacggcgg cttcgcccag agccccggcg gcgcgagtac
     9961 ccacggcgta tgggtcacga acgcttatgt catactcgcc cgatcccggg tctggggcgg
    10021 aatcgccaaa tccagctctg tcgattatga tgccagcgcc gcaggcgtat tcgccgaata
    10081 cggcacccgc acggtggcca tagtcaattc gctggtatca ggcggctact cgagcaatag
    10141 cgccgcgggc ggccgcgagt acctgcgcgg cgtcgaaacc tatcaacaac ctattcgtat
    10201 atgcggctcg gtcatcgact cggggttctg ccccgaaccg gtcgagacct acagctacgt
    10261 ctccgccacc tccaccgagg aaagcggtac ggtgcagatt gagtcgagca tcgtcataac
    10321 ctcgaacgcc acggccttcg gcgacctgtt ctatgacgcc gaaatgccga acagcgcgac
    10381 cacctgctac ggttgcctcg cggcgcaccc tggggaattc tcgtatggcg gctcggacag
    10441 cgcgttcccc gaacctatcg cgggcaacga ggatctcccg aacctagccc tcggcggctt
    10501 cttcacggcg ttttcgaacg cctatacggg aagctatccg tccgactacg agcgttggct
    10561 gggcggctcg tggcgcgttt cgccggggta ctcagcctat atcggcggcg gtaacctgcc
    10621 gtattcaggc ggatcggtgg cgggtttctc agtagccgac tatccggcgc tggtcctcga
    10681 cctggcgggc aaggccaggc cgacgagcgg ttcctggggc cgcggcgcct acgaataatg
    10741 aacgccaaga cgctcctacc gtataggcta agactatgaa cactatgctc atgatgggga
    10801 cacgatggac acgatgaatc gattaacctt agggtcttac cttgttcatc gttcctccat
    10861 cctgtcatca tgttcgaatt agcctattcg ctatcttcat cgcgttctga gcgcctccgc
    10921 gaccctgcta tctcgcgccc agcgtgacga aggccgccag gtcctccgcg tcgaggggga
    10981 tgccgtcggc acggactacg cggccggagg cgttccaccg ggcggcgtcg agcacggtcg
    11041 ccgaacgctc gggccagagg gcggtggcgg cgacgtacag gaacagcctg cagatcggcg
    11101 cgaagatcac ggccgagcgt tcgtcgtcga actcgaacgc gagcgacgcg acgtaggcgc
    11161 cgagcgcggc ggcctggccg gaggaggcgc gggccgagag catcat
//
LOCUS       DAOPHV010000017        10886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_7364_length_10886_cov_4.140707, whole genome shotgun sequence.
ACCESSION   DAOPHV010000017 DAOPHV010000000
VERSION     DAOPHV010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10886
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7364_length_10886_cov_4.140707"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..780)
                     /locus_tag="PLQ29_00950"
     CDS             complement(64..780)
                     /locus_tag="PLQ29_00950"
                     /inference="COORDINATES: protein motif:HMM:NF027796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HPG85237.1"
                     /translation="MTDPRNSPFMYAIGAFVALFVTVQSLVFMIRAYREGKRLGLSAA
                     TMRKAIVGSASFSVVPSIAILLAVLTLSGALGLPLPWIRLSVVGAITYELPAAETAAQ
                     AFGASIKSAITDPVVFSAIAWAMSIGCVFAMFLVIFFSRGIQSGIHKAQLKDSVWSGL
                     LISAMFMGLISAFLGSALGGGLVSILTLLSSAATMGVFGLLIKKAKLTSLENFAMPVS
                     MIVGMAAAAVYSSIAGGRTI"
     gene            complement(967..2382)
                     /locus_tag="PLQ29_00955"
     CDS             complement(967..2382)
                     /locus_tag="PLQ29_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPG85238.1"
                     /translation="MMNIDRKSTLRGLVMALCAVALAACQSAPERADEATAESGASVP
                     AAMLGDWDGELVAGAQKLALRLYIAAGGVSLDVPAQRVAGAKASSVIFADGLLVAEFD
                     AFRASVELRAAEGGGLEAIWAQSGARLPLTFARAGDGSAAAVPAAPAVPARRGESVRF
                     KSGQAGVELAGTLRLPDGKGPFPCLILVSGSGAQDRDEAIGTIMPFRDIAEGMAARGW
                     ATLRYDDRGVGESGGTFAGSTSEDFALDAVGAYRFASADGRIDARRVALAGHSEGGLV
                     IAIAENLLGGVYGLVSIAGPGVGGYEILLDQSRDILAGMGAPAEAIESSQAINRALYD
                     MILESDEFPLEAVKAELSKAGLPPDQLEAQLPMLRDPWFKAFLRSNPAGYYSKVRSPV
                     LAINGSKDSQVRAGKNLAAIEAAVKRAGVPVKAVAMDGLNHLFQQANTGMPDEYMGLP
                     PAFSPEAIALMADWLDRLGSR"
     gene            complement(2363..3061)
                     /locus_tag="PLQ29_00960"
     CDS             complement(2363..3061)
                     /locus_tag="PLQ29_00960"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HPG85239.1"
                     /translation="MKAIDKATIWIGALTAALLAATAVLYARLPVSIPIHWDSAGAAD
                     GWAAKPWAFLLASSPVLATALVRIAAAVDPRYSNIKAREGTFVVVVVSVAALAASLHL
                     ITIVTALGLLEGTDLRLVPLATGLAFVAIGNVMPRLKWNYTVGIRLPWTLADEGVWRA
                     THRFGGVAFVLAGLATAATAFMPERAARFVGGGAILSCVAATTAYSYAVHRSRRDLGR
                     NYIDSEGGNDEHRS"
     gene            complement(3058..3321)
                     /locus_tag="PLQ29_00965"
     CDS             complement(3058..3321)
                     /locus_tag="PLQ29_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HPG85240.1"
                     /translation="MNKIWKALDDPTRRRILELLKAGPRSAGELGAEFDMSAASMSHH
                     LGMLADAGLVEREKKGQFVYYELNSTVFQSLLEWIMAMKGQDR"
     gene            3464..4651
                     /locus_tag="PLQ29_00970"
     CDS             3464..4651
                     /locus_tag="PLQ29_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPG85241.1"
                     /translation="MIHTRRTTALAVIQFTLTMLPLAAWSQALPRDASKAGFIATAGK
                     RMASFEVKSLTGSSLSSKDLAGSPALFHIWSARDDPAGNGPALAASLREALLESRPDA
                     AVIVLSADTDDAKAAIRALGSPSAPAWIFVSPVGEIVAYRLGRLSASQAGTAVEALFG
                     LYPSDSRSVAPVAPVAPVAPVAPAAPAPGTAAQAKVDASAPGTAAQAKVDASASIEER
                     ILAELNLARTKPSAYIEVLKEYRTHIRGNYLERPGETTVVLNEGVRAVDEAIAFLSRQ
                     TALPPLTPSAGLRRAAADHAADQGRTGQTGHAGSDRSTMKSRVERYGTWSRTIGENIA
                     YGAETARDVVIQLIVDDGVASRGHRANIFNPGFLVVGIAFGAHPTYRTVCVQDFAGGY
                     TER"
     gene            4725..5465
                     /locus_tag="PLQ29_00975"
     CDS             4725..5465
                     /locus_tag="PLQ29_00975"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPG85242.1"
                     /translation="MMHANEPDDSTRQSEAVSPPDSREPPLRERVYTYLKLKLNDGAL
                     KPGAFLDLNALGVELGLSRTPLRDALLRLEAEGFVTIHPRRGVVLNPLDLQSIRNSYQ
                     LLGAIEAVAIIEVAPTFAPSDVEKMFGLNSQMREALDRNDFDDYYSANLAFHDSYIAR
                     SSNVELKRLARILKDRLYDFPRRESYLSQWERESLKEHEKLATLLMAGSFEEAAAYVR
                     DVHWSFKVQERYIMAYYFAREAALGPML"
     gene            5565..7937
                     /locus_tag="PLQ29_00980"
     CDS             5565..7937
                     /locus_tag="PLQ29_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPG85243.1"
                     /translation="MTDRIARLRRESVETKPSIDIQRALLITEAYASMPDGLPAPIVR
                     ARSFAYLCEHMDIYIGADDLIVGERGRGPKRTSTYPELTCHSVEDLVTLRSRPMTSYD
                     IGDDDIESYRERVIPYWRGRCVRDRAFAGLPAEWLALYEAGVFTEFMEQRAAGHTALD
                     GLLYRKGLRELKSEAAAARSALSPEDPRLEEKVAELEAMATSCDAMIRRAERHADRAE
                     AMAASERNAARALELRDIAAVCRRVPAEAPRTFHEALQAYWFVHLGTLTELNGWDAMS
                     PGHLDQHLEPFYRRDLADGRLTRERAKELLSAFWIKVNNTPAPPKVGITAAESGTYND
                     FTNINLGGLAPNGRDGSGEVTLLALEVLEELRLLQPQANLQVSSRTPDRVLEAACAVV
                     REGMGYPSLFNADEVTLAQVASGKSLRDAREGGTSGCIETGCFGKEAYILHGYLNGPK
                     LLELALNDGIDPASGRRVGSATGPAESFRSTDELFAAWVAQLRYAVDWKARVDGFLDD
                     LYGRSTPAPFLSLYIADCVSRGLDYYRGGARYNSDYIQCVGLGTVTDSLSAIKTQVFR
                     AGKATMAGLVEALHADWAGSDALRSLVRNRTPLFGNDDDEADAIAARVFDAFVDTVER
                     DGGKPRISRRGAPYRANFLSTTCHVYFGTKTGATPDGRKSGEPLSDGTSPSHGADRLG
                     PTAVMNSLGKLDQAKTGGTLLNLRFSPSALAGADGPKRLARLVRGYFRQGGHHVQFNV
                     VDEATLRAAKRSPSDYRSLLVRVAGYSDYFVDLDERHQDEIISRTAHARA"
     gene            complement(7967..8314)
                     /locus_tag="PLQ29_00985"
     CDS             complement(7967..8314)
                     /locus_tag="PLQ29_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85244.1"
                     /translation="MSAELTAALRLLREHAFLRYAPSGAAEYEEKVRTACGMFASAVS
                     RAAGAPVPNPYPAATIDGLGATGPDAGVRAARRVAAALAKASAANPGSDRLRRAADAW
                     RRASEALYAASWA"
     gene            complement(8342..9562)
                     /gene="tyrS"
                     /locus_tag="PLQ29_00990"
     CDS             complement(8342..9562)
                     /gene="tyrS"
                     /locus_tag="PLQ29_00990"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPG85245.1"
                     /translation="MNRALETLKERGFVQQCTDIEGLSALMDQGPVTFYIGCDPTGPS
                     LHIGHMVPFFAYRHLVEAGHVGIALLGGGTARIGDPSGKSEMRKMISYDAIDANAELF
                     RGQLDSFIGFDGKNRLHDNNKHWLADLNYIEFLRDIGRHFSVNRMLTFEAYRQRMETG
                     LSFIEFNYQLLQSYDFLQLYKRHGCRLQIGGDDQWGNIVAGMELIRRVEGAEAFGLTF
                     PLVMTSDGKKMGKTEKGALFLDPAMTSPYDFFQYWRNVSDGDVLRFLLMFTFMPIDEC
                     RALASGNINAAKERLAWEVTALIHGKDEADKALAGAKAAFGGGGDKSAMPTVELARAR
                     FEAGYPVLDLFVDAGLAPTKSEARRLVQQGGAVVGEAQWKDEKTQVGAAALDADGELV
                     LRAGKKRFVRVVAS"
     gene            9977..>10886
                     /locus_tag="PLQ29_00995"
     CDS             9977..>10886
                     /locus_tag="PLQ29_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPG85246.1"
                     /translation="MATRIETLASILRAAPGSVFIQAHDVPDPDAIASAFGLRHLLSS
                     YGVESTIVYEREFEKIDAQRMVELLGIELSLASDVPTIGPDDWAVIIDGQRGNANMVD
                     LACREVAAIDHHALRDDAAYRYADIRPEVGSCAAMIAEYFAEAGMAPPGLVATALMYG
                     IFVDTDNLTRGVSDLDARMFYELRAWADADLIRKLRGSQITLRDLAMYAEALGKVETH
                     GRLAFLGLRDADDSLVGAANDLVQSVDEVDVSLSYSLREDGIKLSTRSLVEAVRADEL
                     AKAIVDGIGFAGGQLGSTRILGERALA"
BASE COUNT         1913 a         3671 c         3494 g         1808 t
ORIGIN      
        1 ccgccatcca ggcccggccg ttccggtgga tggcctcgtc ataggtcgtc ttcgtcgcac
       61 cgctcatatc gtcctcccgc ccgcgatgga gctatagacg gcggccgcgg ccatgccgac
      121 gatcatgctg accggcatcg cgaagttctc gaggctggtc agcttggctt tcttaatcag
      181 gagcccgaag acgcccatcg tcgcggccga ggacagcagg gtcaggatgg agacgagccc
      241 gccgccgagg gcgctgccca ggaaagcgga gatgaggccc atgaacatcg cgctgatgag
      301 gaggcccgac cagaccgagt ccttgagttg ggccttgtgg atgcccgact ggatgcccct
      361 cgagaagaag atgacgagga acatggcgaa gacgcagccg atcgacatgg cccaggctat
      421 cgccgagaag acgaccgggt cggtgatcgc cgacttgatc gacgcgccga aggcctgggc
      481 ggccgtctcg gcggcgggta gttcgtaggt tatggcgccg accaccgaca gccgtatcca
      541 cggcaggggc aggccgaggg cgccggacag agtcagcacg gcgagcagga tggcgatgga
      601 cggaacgacg gagaagctgg ccgagccgac gatggccttg cgcatcgtcg ccgcgctcag
      661 gccgaggcgc ttaccctccc tgtaggccct gatcatgaag accaggctct ggaccgtgac
      721 gaacagggcg acgaaggccc cgatcgcgta catgaacgga ctgttccgcg ggtcggtcat
      781 ggggacgact ccttggttcg attgttttca gcgatagaaa ctgtgatcga ttaaaccgtc
      841 gcatccggcc gctgtcaacg aaaaagcgcc cggacggcgt cggccatccg ggcgctcgaa
      901 caaagatgcg tcgcgcgatg cgtcgcgcgt tcaaaggcgc gcggatcatg cgccccggcg
      961 ttttacctag cgcgacccga gcctgtcgag ccaatcggcc atcagcgcta tcgcctcggg
     1021 cgagaaagcc ggcggaaggc ccatgtactc gtcgggcatg ccggtattcg cctgctggaa
     1081 caggtggtta agcccgtcca tcgcgacggc cttgaccggc acgccggccc gcttgacggc
     1141 cgcctcgatg gccgccaggt tcttgccggc gcgcacctgc gagtccttgg agccgttgat
     1201 cgcgaggacc gggctcctga ccttggagta gtatccggcc gggttcgagc gtaggaaggc
     1261 tttgaaccat gggtcgcgaa gcatcggcag ctgggcctcg agctgatccg ggggcaggcc
     1321 cgccttggac agctcggcct tcaccgcctc gagcgggaac tcgtccgact cgagtatcat
     1381 atcgtagagg gcccggttga tggcctggga gctctcgatc gcctcggcgg gggcgcccat
     1441 gccggcgagt atgtccctcg actggtcgag gagtatctcg tagccgccga cgccggggcc
     1501 ggctatcgat acgaggccgt atacgccgcc gagcaagttc tccgctatgg cgatcacgag
     1561 gccgccctcg ctgtgtccgg caagcgccac gcgcctcgcg tcgatgcggc catcggccga
     1621 cgcgaagcga taggcgccta ccgcgtcgag cgcgaagtct tccgaggtgg agccggcgaa
     1681 ggtcccgccc gactcgccga cgccgcggtc gtcgtagcgc agcgtggccc agcccctggc
     1741 ggccatgccc tcggcgatgt cgcggaaggg catgatggtg ccgatcgcct cgtcgcggtc
     1801 ctgggcgccc gagccgctga ccaggatcag gcagggaaag ggtcccttgc cgtccgggag
     1861 cctgagcgtg cccgccagct cgacgccggc ctggccgctc ttgaagcgga cgctctcgcc
     1921 gcgtcgtgcc gggaccgcag gggccgccgg aaccgcggcc gccgatccgt cgccggcccg
     1981 agcgaaggtc aacggtaagc gagcgccgct ctgggcccag atcgcctcga gcccgccgcc
     2041 ctcggcagcg cgcagttcca ccgaagccct gaacgcgtcg aactcggcga cgagtagccc
     2101 gtcggcgaag atgaccgagg aagccttggc cccggcgacg cgttgggccg gtacgtcgag
     2161 cgagacgccg ccggccgcta tatagagcct gagcgccagc ttctgggcgc cggcgacgag
     2221 ctccccgtcc cagtcgccca gcatggctgc cggtaccgag gcaccgctct ccgcggttgc
     2281 ctcgtcggcg cgctccggag cgctctggca cgcggccaaa gctacggcgc acagcgccat
     2341 gacgaggccg cgtagcgtcg atttacgatc tatgttcatc attccctcct tcagaatcga
     2401 tgtagttccg gccgaggtca cgtcgcgatc ggtgtacggc gtacgagtag gccgtggtcg
     2461 ccgcgacaca ggataggatc gcgcctccac cgacaaaccg agccgcccgc tctggcatga
     2521 aggccgtcgc ggccgtcgcc aggccggcca gcacgaaggc gacgccgccg aagcggtgcg
     2581 tagccctcca gacgccttcg tcggccagcg tccagggcag gcggatgccg accgtgtagt
     2641 tccacttgag gcgcggcatc acgttgccga tggcgacgaa ggccaggccg gtcgcgagcg
     2701 gcacgagcct caggtcggtg ccttcgagca gtccgagggc ggtcacgatc gttatcaggt
     2761 gcagggaggc tgccagcgcg gccaccgata cgacgacgac gacgaaggtc ccctcgcgcg
     2821 ccttgatgtt gctgtagcga gggtcgacgg ccgccgcgat ccggaccagt gcggtcgcca
     2881 gtacgggcga cgaggctagc aggaaggccc agggtttggc ggcccagccg tcggccgcgc
     2941 cggccgagtc ccagtgtatc ggtatcgata cgggcaggcg cgcgtacagg accgcggtcg
     3001 ccgccaagag cgccgcggta agcgcgccta tccagatagt cgccttgtcg atagctttca
     3061 tcgatcctgt cctttcatcg ccataatcca ctcgagcagg ctctggaaca cggtcgaatt
     3121 gagctcgtag tagacgaact gacccttctt ctcccgctcg acgagcccag cgtcagccag
     3181 catgccgaga tggtggctca tcgaggcagc gctcatatcg aattctgcgc cgagctcgcc
     3241 ggcgctcctc ggacccgcct tgaggagctc gagtatccgt cgcctcgtcg ggtcatcgag
     3301 agccttccaa atcttgttca ccgtgttcgc ggctcctatc gcttagatat ttagaatatc
     3361 ttctaaatat cttaagacgt caagccgacg attgaggatt ccgcgctagc cgtttcggcg
     3421 ctatagtaga agcgaacgcg ccgaccgctc aaggaggcga cgaatgatcc atactagacg
     3481 cacgacggcc ctagccgtca tccagtttac cttgaccatg ctaccgctcg cggcctggag
     3541 ccaggcgttg ccccgcgatg cctcgaaggc aggtttcatt gcgaccgccg gtaagcgcat
     3601 ggcttcgttc gaagtcaaga gcctcacggg ctcgtcactg agctccaagg acctggctgg
     3661 ctcgcccgcg ctgttccata tctggtccgc gcgcgacgat cctgccggaa acggtccggc
     3721 cctcgcggcg tccttgcgag aagccctgct cgagtcccgg cccgacgcgg ccgtcatagt
     3781 actgagcgcc gatacagacg acgccaaagc cgcgatccgg gcgctcggta gcccgtccgc
     3841 gccggcctgg atcttcgtct cgccggtcgg cgagatcgtc gcctaccgtc tcggccgcct
     3901 gtccgcgagc caggccggca cggcggtaga agcgttgttc ggcctctatc cgtcggatag
     3961 ccgctcggtc gcaccggtcg caccggtcgc accggtcgca ccggtcgcac cggccgcgcc
     4021 ggcccctgga acggccgctc aggccaaagt cgacgcgtcg gcgcctggaa cggccgctca
     4081 ggccaaagtc gacgcgtcgg cctccatcga ggagcgcatc ctcgccgagc tcaacctggc
     4141 caggacgaag ccttcggcgt acatcgaggt gcttaaagaa tatcgtaccc atatccgagg
     4201 caactacctc gagcgcccag gcgagacgac cgtcgtgctc aacgagggcg tccgggccgt
     4261 cgacgaggcc atagccttcc tatccaggca gaccgccctg ccgccgttga cgccatcggc
     4321 gggcctccgc cgcgcggccg ccgaccacgc ggcggatcag ggcaggaccg gccaaaccgg
     4381 ccacgccggc tccgatcgca gcaccatgaa gtcgcgggtc gagcgttacg gtacgtggag
     4441 ccggaccatc ggcgagaaca tcgcctacgg cgccgagacg gccagggatg tcgtcatcca
     4501 gctgatcgtc gacgatgggg tcgcctctcg cggccaccgg gcgaacatct tcaatcccgg
     4561 gttcctcgtg gtcggcatag ccttcggagc ccatccgacc taccgcacgg tctgcgtcca
     4621 ggacttcgcc ggaggttaca ccgaacgcta ggggcgatgc tccatccctg gtgttctgaa
     4681 gattaaaaga ttgacgaaga ctgatatatc aagataatat cagcatgatg cacgcaaacg
     4741 aaccggacga tagcactcgc cagagcgaag ccgtctcacc gcctgactcg agggagcccc
     4801 cgctacggga gcgggtctac acgtacctta agctgaaact gaatgacggg gcgctcaagc
     4861 ccggtgcctt cctcgacctc aacgcgctcg gcgtcgagct cgggctatcc aggacgcctc
     4921 tgcgcgacgc cttgcttagg ctcgaggccg agggcttcgt gaccatccat ccaaggcgcg
     4981 gcgtcgtgct caatcctctg gatttgcaat cgatacgcaa ctcataccaa ttgctcgggg
     5041 cgatcgaggc ggtcgccatc atcgaagtcg caccgacctt cgccccttca gacgtcgaga
     5101 agatgttcgg cttgaacagt cagatgcgcg aagccctcga ccgtaacgac ttcgacgatt
     5161 actactccgc caatctcgcg tttcacgact cgtatatcgc cagaagctcc aacgttgaac
     5221 tcaagcgcct agcccgcatt ctcaaggata ggttgtacga tttcccccga agggaaagct
     5281 acctgagcca atgggaacga gagagcctca aggagcacga gaagttggct acgctcttga
     5341 tggcgggatc attcgaagag gctgcggcct atgtacgaga cgtccattgg tcgttcaagg
     5401 tccaggagcg ctatatcatg gcctactact tcgccaggga ggccgcactc ggcccgatgc
     5461 tttagcagta tgtataatat tgttaatgaa tttgccgtag gacaggccga acaatgcgct
     5521 tgtgtactat attgcataca tgacgatacg gcatggagac gagtatgacc gataggattg
     5581 cgcgattgcg acgcgagagc gtcgagacga agccgagcat cgatatccag cgtgctctct
     5641 tgatcaccga agcctacgcc tcgatgcccg acggcctacc cgccccaatc gtccgggcgc
     5701 gttccttcgc ctacctctgc gagcatatgg acatctacat cggagccgac gatctgatcg
     5761 tcggcgaacg cgggcgcggc cctaagagga cctcgaccta tcccgaatta acctgccaca
     5821 gcgtcgaaga cctagtgacg ctgcgttcga gaccgatgac cagctacgat attggcgacg
     5881 atgatatcga gtcgtaccgc gaacgggtca tcccctactg gcgcggccgc tgcgtccgtg
     5941 accgcgcctt cgcaggcctt ccggccgagt ggctcgccct ctacgaggcg ggcgtcttta
     6001 ccgagttcat ggaacagcga gccgccggcc ataccgcgct cgacggactc ctctaccgca
     6061 agggcctgcg agagctcaag tccgaagccg cggcggcccg ttcggctcta tcgcccgagg
     6121 atccaaggct cgaggaaaag gtggccgaac tcgaggccat ggccacctcc tgcgacgcta
     6181 tgatcaggcg ggccgagcgc cacgccgatc gggccgaggc gatggccgcc tcggaacgga
     6241 acgccgcccg cgcgctcgag ctacgggaca tagccgcggt atgccgcaga gtaccggccg
     6301 aggccccgag gaccttccat gaggcgctac aggcctattg gttcgtgcat cttggcacgc
     6361 tgaccgagct caacggctgg gacgccatga gtccgggcca tctcgaccag cacctcgaac
     6421 cgttctaccg gcgcgacctc gccgacggcc gcctgacccg cgagcgggcc aaggagctcc
     6481 tgtccgcctt ctggatcaag gtgaacaaca ctccagcccc accaaaggtc ggcatcaccg
     6541 ccgcagagtc gggcacctat aacgacttta ccaacatcaa cctcggcggc ctcgcgccca
     6601 acggccgcga tggctccggc gaggtgacgc tcctggccct cgaggtgctt gaggaactca
     6661 ggctgctgca accgcaggcg aacctgcagg tctcgtccag gacgcccgat cgcgtgctcg
     6721 aggccgcctg cgccgtcgtc cgcgagggca tgggctatcc gtcgctgttc aacgccgacg
     6781 aggtgacgct ggcccaggtc gcctccggga agagcctgcg cgacgcgcgc gagggcggca
     6841 cctcaggctg catcgagacc ggctgcttcg gcaaggaagc ctacatcctc cacggctacc
     6901 tgaacgggcc caagctcctc gagctggccc tgaatgacgg tatcgacccg gccagcggcc
     6961 gacgcgtcgg gtccgcgacc ggcccggccg agtcgttccg ctcgaccgac gagctgttcg
     7021 cggcctgggt cgcccagcta cgctacgccg tcgactggaa ggcccgcgtc gacggcttcc
     7081 tcgacgacct gtacggccgg agcacgcccg ctccgttcct gtcgttgtac atcgccgact
     7141 gcgtctcgcg cggcctcgac tactatcgcg gcggcgcccg ctacaatagc gactatatcc
     7201 agtgtgtcgg cctcggcacc gtgaccgatt cgctgtcggc catcaagacc caggtcttca
     7261 gggccggcaa agcgacgatg gccggcctcg tcgaggcgct ccacgctgac tgggctggta
     7321 gcgacgcact gcgctccctg gtcaggaacc gaacgccgct gttcggcaac gacgacgacg
     7381 aggccgacgc gatcgcggcg cgggtcttcg acgccttcgt cgacacggtc gaacgggacg
     7441 gcgggaagcc gaggatatcg aggcggggcg ccccgtatag ggccaatttc ctgtcgacga
     7501 cctgccacgt ctacttcggt accaagaccg gcgccacgcc cgacgggcga aagtccggcg
     7561 agccgctatc ggacggcacg agcccctcgc acggcgccga caggctcggg cccacggcgg
     7621 tgatgaacag tcttggaaag ttggaccaag ccaagaccgg cgggaccctg ctcaacctgc
     7681 gcttctcgcc gagcgccctg gccggcgccg acggcccaaa gcgcctggcc aggctcgttc
     7741 gcggctactt caggcaaggc gggcatcatg tgcagttcaa cgtcgtcgac gaggccacgc
     7801 tcagggcggc caagcggagc ccgagcgact atcgcagcct cctcgttcgc gtggccggct
     7861 actcggacta cttcgtcgat ctggacgagc gccaccagga cgaaatcatc tcccgcacgg
     7921 cccacgcgcg ggcctgatcc ggaatacgct caggcgcgcc gagggctcaa gcccacgacg
     7981 cggcgtacag ggcctcggaa gcccgccgcc acgcatcggc cgccctccgc agccgatccg
     8041 agcctggatt cgcggcggac gccttggcca gggccgcggc cactcgccga gccgccctga
     8101 cccccgcgtc cggtcccgtg gcaccgaggc cgtctatcgt cgcggcagga tacgggttcg
     8161 gcacgggcgc gcccgcggcc ctcgagacgg ccgacgcgaa cataccacag gcagtgcgca
     8221 ccttttcctc gtactctgcc gcgcccgacg gggcgtagcg caggaacgcg tgctcgcgga
     8281 gcaacctaag ggccgcagtg agttctgccg acaattccga gcgcgtcagg atgccgcctc
     8341 cctacgaggc gaccacgcgg acgaagcgct tctttccggc gcgcagcacg agctcgccgt
     8401 cggcgtcgag cgctgccgct ccgacctgcg tcttctcgtc cttccactgc gcctcgccta
     8461 cgacggcgcc gccctgctgc acgagcctgc gcgcctcgct cttcgtcggc gcgagccccg
     8521 cgtcgacgaa cagatcgagc accgggtagc ctgcctcgaa gcgggcgcgc gccagctcga
     8581 cggtcggcat cgccgacttg tcgcctccgc cgccgaacgc ggccttggcg ccggccaggg
     8641 ccttatccgc ctcgtccttg ccatggatta gcgccgtgac ctcccaagcg agccgctcct
     8701 tggcggcgtt gatgtttccc gaagccaggg cgcggcactc gtctataggc atgaacgtga
     8761 acatgaggag gaacctcagc acgtcgccgt ctgacacgtt ccgccagtac tggaagaaat
     8821 cgtaggggct cgtcatcgcc gggtcgagga acagggcgcc cttctcggtc ttgcccatct
     8881 tcttgccgtc gctggtcatc acgagcggga aggtcagtcc aaaggcctcg gcgccctcga
     8941 cgcggcgaat cagctccatg ccggcgacga tattgcccca ctggtcgtcg ccgcctatct
     9001 ggagcctgca gccgtggcgc ttgtacagct gcaggaagtc gtagctctgt agcagctggt
     9061 agttgaactc gatgaacgac aggccggtct ccatgcgctg cctgtacgcc tcgaaggtca
     9121 gcatgcggtt gaccgagaag tgcctgccta tgtcgcgcag gaactcgatg tagttcaggt
     9181 cggccagcca gtgcttgttg ttgtcgtgca ggcggttctt gccgtcgaag ccgatgaaac
     9241 tgtcgagctg cccgcggaac agctcggcgt tcgcgtctat cgcgtcgtag gagatcatct
     9301 tgcgcatctc gctcttgcct gacgggtcgc ctatcctggc cgtgccgccg ccgagcagcg
     9361 ctatgccgac gtggcccgcc tcgacgaggt ggcggtaggc gaagaacggg accatgtgcc
     9421 cgatatgcag cgatggaccg gtcgggtcgc accctatgta gaaggtgacg ggcccctggt
     9481 ccatcagggc ggagagcccc tcgatgtcgg tgcattgctg tacgaagccg cgttccttca
     9541 gggtttccag ggctcgattc atgccgttcc tccgtagaat actcgtgtgg cgcgcgggga
     9601 ttatagccac tgcgccgaat gcgcggcaag ggccgcgacg cgcgctgggg cgaataaggc
     9661 taactgaacg aagagactat tcgtatacaa acgggaaggc gtatacgacg aacggcggaa
     9721 catgatgtcc agtattttct cgataaaata ggctgccgtt tgtacggtaa gcaaagcacc
     9781 tagacgccat cgtggtcatc atacgccatc cagctcatca tattctgtcg ttccggggcc
     9841 ggtccttcgt agcccgctcc gttgctctgt gcctccgtgg atcaaaatcc ctcgtatcgc
     9901 tcgtacctgc cttgcgcgtt gggggcatcg ttggtagcat ctgtggctag ggacgctcgg
     9961 agcgacggag gcatgcatgg caacgaggat agagacgctc gcttcgatac tgcgggccgc
    10021 gccggggtcg gtgttcatcc aggcccacga cgtgcccgac cccgacgcca tcgcgtccgc
    10081 cttcggcctc cgccacctgc tgtcgagcta cggcgtcgag tcgaccatcg tctacgagcg
    10141 agaattcgag aagatcgacg cccagcgcat ggtggagctc ctgggcatcg agctgtccct
    10201 ggcctcggac gtgccgacga taggcccgga cgactgggcc gtcatcatcg acgggcaacg
    10261 cggcaacgcc aacatggtcg acctcgcctg ccgggaggtg gccgcgatag accaccacgc
    10321 cctccgcgac gacgcggcgt accgatacgc cgacatacgt cccgaggtgg gctcctgcgc
    10381 cgcgatgata gccgagtact tcgccgaggc cggcatggcg ccgcccgggc tcgtggccac
    10441 ggccctcatg tacggcatct tcgtcgacac tgataacctg acccgcggcg tctccgacct
    10501 ggacgccagg atgttctacg agctccgggc ctgggcggac gccgacctga tacggaagct
    10561 acgcggcagc cagattacgc tcagggatct ggcgatgtac gccgaggcgc tcggcaaggt
    10621 cgagacgcat ggtcggctcg ccttcctggg cctgcgggac gccgacgact cgctggtcgg
    10681 agccgccaac gacctcgtgc agtcggtcga cgaggtggac gtctccctgt cgtactcgct
    10741 acgcgaagac gggatcaagc tgtcgacgag gagcctcgtc gaggcggtcc gggccgacga
    10801 gctggccaag gctatcgtcg acggtatcgg tttcgccggc ggccaactcg gctcgacgcg
    10861 gatcctcggc gagcgggcgc tggcgt
//
LOCUS       DAOPHV010000018        10877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_7375_length_10877_cov_4.340233, whole genome shotgun sequence.
ACCESSION   DAOPHV010000018 DAOPHV010000000
VERSION     DAOPHV010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10877
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7375_length_10877_cov_4.340233"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PLQ29_01000"
     CDS             <1..653
                     /locus_tag="PLQ29_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HPG85247.1"
                     /translation="FVQEAKPGFLGRLFGRKRIVTLLEASPEEGKRVARAHQGVEVLQ
                     VDSAEEGVLERSGVGRADLVVCATESQSFNVITAQLAKQLGAKKGVAVILSDRYMPLG
                     AKLDVDALVSIKGVVAAAVLETVRRANIRTIHGFYEDDVELVELTVGAASTVAGRSLR
                     EIDLPKGVLVAFVIHGEAMLVPTGATTLNVGDVLGLVSRKKSIPGLERVFGGDRGA"
     gene            643..2100
                     /locus_tag="PLQ29_01005"
     CDS             643..2100
                     /locus_tag="PLQ29_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPG85248.1"
                     /translation="MELKPLAKALAVLLAIVALFMAGAMVAGLLLGEGSAAVGAFAPS
                     VAIGLGALVATQALIRFRSGLSLSHRSGYLFVTLAWVLSAALGALPFRLYGAVPSYTD
                     AFFETMSGFSTTGASILADIESLPESLLLWRAMTHWLGGMGIVVLTVAIMPLLGLSAR
                     SVMEAEAPGPQVDKFTPRLSQTAKILWLIYLGLTAALTVLLLFGGMSLIDALTHAFAT
                     MATGGFSTRNASVGAFGSTYVDVVLTVFMVLAGVNFSLHWSALRGDLRKVLADTELRV
                     YLAVFAAASVVIVLDLRRAGLFDSVGEAARYASFQASSILTTTGFATADYTLWPPLSQ
                     AVLFTLMFIGGCAGSTGGGVKVSRIVTLFKMGQSEMRYLLNPRGVYGVFLDGRHLKKN
                     VVYDIAAMVFLYLVAAFVSTMAVAAGGYDILTSLSATMACLGNIGPGFALVGPACNYG
                     FMPGALKWWLSFAMLLGRLEVYTVLVLFTRAFWRR"
     gene            complement(2109..3377)
                     /locus_tag="PLQ29_01010"
     CDS             complement(2109..3377)
                     /locus_tag="PLQ29_01010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPG85249.1"
                     /translation="MLFLLAGLSQTGRLRQYERSAVLLGASTVRVAIEGSLNEHISLE
                     RGIVAFVASDTGLDSEAYDRYARALAMDDPIIINLAVLEGTVIKFVYPYDQNANAIGK
                     DLAAVPDQAAAIVRATTTRDPIVSGPHELVQGGYGVISRMGIFPIGPSGPEYWGQASV
                     VVDVNEILRRSGVAEHDALSFLLCAGAEGATDAATIFGDRAVLGQDPVVLDIRLPGAT
                     WTLAAVPKGGWKSFGWLTFGGAWIGAVIGLLAGFALFSLITTRGALKELAYHDQLTNL
                     PNRTLFWDRLHVEAARAERDGQRVCVCMVDLDGFKAVNDDLGHESGDRLLKQAAARMS
                     EALRRSDTVARLGGDEFAVIAQVDSPSGVDEVTARIKGCFAEPFDLGSVKRASSASIG
                     GALYPDDGTDVEAVLAKADHRMYARKHAAR"
     gene            3799..4854
                     /locus_tag="PLQ29_01015"
     CDS             3799..4854
                     /locus_tag="PLQ29_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG85250.1"
                     /translation="MPAIIARDLSKTFKTKVRPSGRLAALRSLVKPEYRDVRAVKGVS
                     FEIDDGELVAFLGPNGAGKSTTIKMLMGILRPTGGSATVLGLDPAEDRVELSRYVGAV
                     FGQKSQLWFHLPPSDSFRLLGSIYEIDDDDRRWREAELIERFGLAPYWDVPVRKLSLG
                     ERIRCELAASLLPSPVALFLDEPTIGLDVVAKREIRQLLAEAARVDDTTIFMTSHDMG
                     DIEKVCKRAIIIHHGEVVVDESMKALKHRSLSRKYVGVRYASPVDLELPGLSPVKRTP
                     TAASFEVDTSRHSLAEVVRALASRGDLEDITVEDEPLENVIAAIYETRSGAEAQALGL
                     NASKGAGAARAGEAASA"
     gene            4847..5677
                     /locus_tag="PLQ29_01020"
     CDS             4847..5677
                     /locus_tag="PLQ29_01020"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HPG85251.1"
                     /translation="MRSRCYAIAASTSMRERAAYPGNFIGSMFTYALFVFIFSRVWAG
                     AYAGASEIAGYTMRMAVWYFIVAEVPSFGFGRFFWTLSRDMKNGQIAYLLARPYDYVG
                     YNFAEKYGGSLVEAGVILVEGLLVGILLVGPPPLAGGAPSLAGGAGVAAEASRALLVG
                     LSLLLAGALNYFLQASIAMTAFWLEENEAFFWIFQKLTLVVGTLIPIEFLPTAAATLA
                     RLTPFPYLSYAPARIMVAYSPGEAWSLVAAQALWLVLAAALARIVFSFGSRRLSVNGG
                     "
     gene            5679..6509
                     /locus_tag="PLQ29_01025"
     CDS             5679..6509
                     /locus_tag="PLQ29_01025"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HPG85252.1"
                     /translation="MLGTGRNGPGRLGGRTRAYARFLRDAFVVNLKATLEYRVNFIMQ
                     FFGMMLNNAAFAAFWLVLIGRTGAVGGYGFSDVMLIWALVSTSFGLAHVVFGNVRQLG
                     RIIMEGGLDVYLLQPKDAWINVLASRTVVSAWGDLAYGFIVLALMNGTNLERLALFTA
                     LAVPGALIFAATFAAAESLTFFMGNSSAVSSALTEFMLSFSLYPEGIYGHGMRWILYS
                     IVPSGFVAFMPLRVFRSLDWGLVPLLYLVAAAYVGLSYWLFRAGLRRYESGNRMDARV
                     "
     gene            6548..7321
                     /locus_tag="PLQ29_01030"
     CDS             6548..7321
                     /locus_tag="PLQ29_01030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG85253.1"
                     /translation="MLVQKARGSALPAEIYALFAVRLVVSAGSFVGPFLAMMLTMKLG
                     YDEARAGAFMSVVSVASALGLVVGGKLGDAFDRPRVLRILQATTAAAYVVCAVMGFNA
                     ATPFVIAFAMGTLNGTWPIINAIVADAAPAERRKEAFSLLYWGNNIGFSIGLFIAGLL
                     FTRAPQALFAGNAAVLFAAAIIVARYVRGGARTEAPVGVPTIDRQATAAAAADGPIAC
                     PADVQPTCSRPRPPRPLRRPSGSCSGVARCCCCTRPDPC"
     assembly_gap    7324..7423
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7424..8036
                     /locus_tag="PLQ29_01035"
     CDS             <7424..8036
                     /locus_tag="PLQ29_01035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85254.1"
                     /translation="VFRRSPVLLLYSAGSVLTAFVYNQHTFALPIFLNDALGEVAGPR
                     VFGAAMMANGLTVVALTALVVLASKRLRSLTSVALASAFYAVGFGAYYLVGGSRYGVP
                     LALGATVVWTVGEILGSTNGNAFVAEKAPPAYRSRVNSVVSFSYIAGGALAPLAAGSI
                     ARAYGSAAVWPFVAASAALSAAFMYGVDRLDKKTGARVTAAGS"
     gene            complement(8017..9171)
                     /locus_tag="PLQ29_01040"
     CDS             complement(8017..9171)
                     /locus_tag="PLQ29_01040"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HPG85255.1"
                     /translation="MNALFALAFLVAPALIVYLCGHFKPLRTIGAVLLCYAAGVVVGN
                     VGVLPEGFAPVQSAMADVSVVLALPLLLFSIDVRAWFKVAGKGMLCMALAALSIIAVT
                     FALQLTFHSGRPDAWQLAGLAVGVYTGGTPNLAAIKAAIGVANDTYILFHTYDAVISF
                     AYIVVMASVARPILLRVARMRGFEPAAGAPETGGDMANDAAGISFREPGAVRGLVLAS
                     LLSAAIVGASAFLGSLAPGTGGTAVTILAVTTLSLAASFIGPVRRVRHTFQAGMYVIY
                     VFCFVVASMTKLDSLVRVDWAVLGYVSASIFGSLVVHALLSKLAGIDVDTFIVTSVSA
                     ICSPPFVPVLAEALKNRAVLMTGLTTGLVGYAAGNYLGVSIAYLFRSLPL"
     gene            9307..10296
                     /locus_tag="PLQ29_01045"
     CDS             9307..10296
                     /locus_tag="PLQ29_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85256.1"
                     /translation="MDDNGFCFRPLRASDKAAVMAISAKIWEGDDYLPHSFDDWVSDP
                     DGLFNGCFVGDRLVGFGRVGFVAPGHAWLEGLRKDPDAGVSGVGRAVAVEGLRFLSGV
                     HDLRSIRFSAYAHNPQTIAVNEALGFRRAGAWSIKSKRLPGSADGKEAFGAPAAAPSD
                     RSGVSVRRVDPLDASAAGRIRAALTTGPWAAGFRLESWKAYPLEASPAIREDDLILEA
                     VGSDGSVLGRLIGSLDPLKAQANVVAFGLEPSSPSDASSASSDDLAAAALFAAFERIA
                     SDAGYAYFEAIAPGLAGQREALDACGYESWESEDDFLLYEWPAEWLGELARPR"
     gene            complement(10293..>10877)
                     /locus_tag="PLQ29_01050"
     CDS             complement(10293..>10877)
                     /locus_tag="PLQ29_01050"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HPG85257.1"
                     /translation="TYCYRVAAAVGLACLPVLRLGAGWYDDWSTILAAPDGFVHRMTS
                     VDGAGVVTEYDDLLSGRVTELGPVLFSLPDVRESGFGDGYNVVVHEMAHKLDERDGGL
                     DGCPPLPRSMSRRAWRAAFGDAYADFQVRVARSSSGRRVGRASRLPLDEYAAESPDEF
                     FAVACEAFFDAPQRLEAAYPEVYELLGRFFSGGA"
BASE COUNT         1597 a         3686 c         3662 g         1832 t
ORIGIN      
        1 gcttcgtcca ggaggcgaag cccggtttcc tggggcggct cttcgggcgc aagcgcatcg
       61 tcaccctgct cgaggcgtcg ccggaggagg gcaagcgcgt ggccagagcc catcagggag
      121 tcgaggtgct ccaggtggac agcgccgagg agggcgtcct cgagcgctcg ggtgtgggca
      181 gggccgacct cgtcgtctgc gccacggagt ctcagtcgtt caacgtgatc accgcccagc
      241 tcgccaagca gctcggcgcc aagaagggcg tcgccgtcat cctgagcgac cgctacatgc
      301 cgctcggcgc caagctcgac gtcgacgcct tggtcagcat caagggcgtc gtggccgccg
      361 ccgtgctcga gacggtacgc cgagcgaaca tcaggacgat ccacggcttc tacgaggacg
      421 acgtcgagct cgtcgagctt accgtcgggg ccgcctcgac ggtcgccggg cggtcgctca
      481 gggagatcga cctgcccaag ggcgtgctcg tggccttcgt gatccacggc gaggccatgc
      541 tggtgccgac cggggccact accctcaacg tcggcgacgt gctcggcctc gtgtcgcgca
      601 agaagagcat tccagggctc gagcgagtct tcgggggcga ccgtggagct taagccgctg
      661 gccaaggccc tggcggtgct gctggccatc gtcgcgcttt tcatggcagg ggcgatggtc
      721 gccggcctcc tcctgggcga gggctccgcc gcggtcgggg ccttcgcgcc gtcggtcgcc
      781 atcggcctcg gggctctcgt cgccacgcag gcgctgatac ggttcaggag cggcttgtcg
      841 ctatcccatc gctccggcta cctgttcgtc accctcgcct gggtcctgtc ggccgcgctc
      901 ggcgccttgc cgttcaggct ctacggcgcc gtgccgtcgt atacggacgc gttcttcgag
      961 accatgtccg gcttctcgac gaccggcgcc tccatcctgg cggacatcga gtcgctgccg
     1021 gagtcgctgc tcctgtggcg cgccatgacc cactggctcg gcggcatggg catcgtagtg
     1081 ctgacggtgg ccatcatgcc gctgctcggc ctgagcgcca ggtcggtcat ggaggccgag
     1141 gcgccgggcc cccaggtgga caaatttacg ccgaggctgt cccagaccgc caagatactc
     1201 tggctgatct acctgggcct gacggcggcg ctgaccgtac tcttgctgtt cggcggcatg
     1261 tctctgatcg acgcgctgac ccacgccttc gcgacgatgg ctaccggcgg tttctcgacg
     1321 cgcaacgcca gcgtcggcgc cttcggctcg acctacgtcg acgtcgtgct gacggtcttc
     1381 atggtgctcg ccggcgtcaa cttctcgctg cactggagcg cgctgcgcgg cgatctcagg
     1441 aaggtgctcg ccgacaccga gctcagggtg tacctggccg tcttcgccgc ggcgtcggtc
     1501 gtcatcgtcc tcgacctgcg ccgggccggc ttgttcgact cggtaggcga ggcggccagg
     1561 tacgcgtcgt tccaggcctc gtccatattg acgacgacgg gcttcgcgac ggccgactat
     1621 acgctctggc cgcctctatc ccaggccgtg ctgttcacgc tgatgttcat aggcggctgc
     1681 gccgggtcga ccggcggcgg cgtcaaggtc tcgaggatag tcacgctgtt caagatgggc
     1741 cagtcggaga tgcgctacct gctcaacccg cgcggcgtct acggcgtgtt cctggacggt
     1801 aggcacctca agaagaacgt ggtctacgac atcgccgcca tggtgttcct gtatttggta
     1861 gcggccttcg tctcgacgat ggcggtagcc gccggcggct acgatatcct gaccagcttg
     1921 agcgcgacga tggcctgcct cggcaacatc ggcccgggct tcgccctcgt cggcccggcc
     1981 tgcaactacg gtttcatgcc cggcgcgctc aagtggtggc tgtccttcgc gatgctgctc
     2041 gggcggctcg aggtctacac ggtgctcgtg ctattcaccc gggccttctg gcggcgctga
     2101 ggctcgcctc agcgcgccgc gtgcttcctg gcgtacatgc ggtggtcggc tttcgcgagg
     2161 acagcctcga catcggtgcc gtcgtcggga tagagcgcgc cgccgatgct cgccgacgag
     2221 gcccgcttga ccgagccgag gtcgaacggc tcggcgaagc aacccttgat ccgggccgtc
     2281 acctcgtcga cgccggacgg ggagtctact tgggctatca ccgcgaattc gtcgccgcca
     2341 agccgcgcca ccgtgtcgct cctgcgcagg gcctcgctca tcctcgccgc cgcctgtttc
     2401 aggagccggt cgcccgactc gtgccccagg tcgtcgttga ccgccttgaa gccgtcgagg
     2461 tcgaccatgc agacgcagac ccgctggccg tcccgctcgg cccgcgccgc ctcgacgtgg
     2521 agcctgtccc agaacagggt ccgattcggc aggttggtca gctggtcgtg gtaggccagc
     2581 tccttgaggg cgccgcgggt cgtgatcagg ctaaagagcg cgaagccggc gaggagcccg
     2641 atcacggccc ctatccacgc accgccgaag gtgagccagc cgaagctctt ccagccgcct
     2701 ttcggcacgg cggcgagggt ccacgtggcg cccggcaggc gaatatcgag aaccaccgga
     2761 tcctgcccga gcacggcccg gtcgccgaag atcgtggcgg cgtccgtggc cccctcggcg
     2821 cccgcgcaga gcaggaagga cagagcatcg tgctccgcga cgccggagcg cctgagtatc
     2881 tcgttgacgt cgacgacgac gctggcctgg ccccagtact cggggccgct cggcccgatc
     2941 gggaagatcc ccatgcggct gatgacgccg tagccgccct gcacgagctc gtgggggccg
     3001 gagacgatcg ggtccctcgt cgtcgtggcg cggacaattg ccgcggcctg gtcgggtacg
     3061 gccgctaaat ccttgcctat cgcgtttgcg ttttgatcgt acggatatac gaatttgatc
     3121 acggtgccct cgagcaccgc caggttgatg atgatggggt cgtccatggc gagggctcgg
     3181 gcgtagcggt cgtacgcctc cgaatccaat cccgtgtccg acgcgacgaa ggcgacgatg
     3241 ccgcgctcga ggctgatatg ctcgttcagg ctaccctcga tggccacgcg gacggtcgag
     3301 gcgccgagca ggacggcgct gcgttcgtat tgcctgagcc ttccggtctg cgacagcccg
     3361 gccaacaaga agagcatgaa aatgccgacg aacgcggcgg ctcccgcgag gacggctctc
     3421 gtcggcttac gaggcgctaa tggtcccagc tgcgtactcg atcgatgcat ggctcaagcg
     3481 tatagtggcc gggcgctcgt tgcaagccac gatcacgtcg tggtagctct ccatatagaa
     3541 acacttcgct cgattgactg aaccgacccg cggggggtaa agattacgta gcactttcga
     3601 gagggcgcct gggggttcct cccgttccga gcgggacggg gatgagaccg agcggcgccg
     3661 gcggccgcgt gacggccgcg acacccacgg gataaaagcc cgaggacgga gtctcgttag
     3721 attacggggt ccgtccgccc gccctaccgt agcgtatggc tcaagcgtcg aacggccccg
     3781 ctctagcgga ggctccccat gcccgcgatc atcgcgcgcg atctatccaa gacctttaag
     3841 acgaaggttc gcccgtcggg gcgactcgcg gctctcaggt cgctcgtcaa gcccgagtac
     3901 cgcgacgtcc gagccgtgaa gggcgtgtcc ttcgagatcg acgacggcga gctcgtcgcc
     3961 ttcctggggc cgaacggcgc gggcaagtcc acgaccatca agatgctcat gggcatcctc
     4021 aggccgactg gaggctcggc gaccgtgctc gggctcgacc cggccgagga ccgcgtcgag
     4081 ctgtcgcgct acgtcggcgc ggtgttcggc cagaagagcc agctgtggtt ccacctgccc
     4141 ccgtccgact cgttcaggtt gctcggctcg atctatgaga tagacgacga cgaccgcagg
     4201 tggcgcgagg ccgagctgat cgagcgcttc ggcctggcgc cgtactggga cgtgccggtc
     4261 cgcaagctgt ccctgggcga gcgtattcgc tgcgagctag ccgcgagcct cctaccgtca
     4321 ccggtcgcgc tgttcctgga cgagcctacc atcggcctcg acgtggtggc caagcgagag
     4381 attcggcagc tgctcgccga ggcggccagg gtggacgaca cgaccatctt catgaccagc
     4441 cacgacatgg gcgacatcga gaaggtctgc aagcgggcca tcatcatcca ccacggcgag
     4501 gtggtcgtcg acgagtccat gaaggccctg aagcaccgct ccctgtcgcg caagtacgtc
     4561 ggcgtgcgct acgcctcgcc cgtggacctc gagctacccg ggctgtcgcc ggtcaagcgc
     4621 acgccgacgg ccgccagctt cgaggtggac acgagccggc attccttggc cgaggtcgtg
     4681 cgggcgctcg cctctcgcgg cgacctggag gacattacgg tagaggacga gccgctcgag
     4741 aacgtcatcg cggccatcta cgagacgcgt agcggcgccg aggcccaggc gctcggcctt
     4801 aacgcctcga agggcgcagg cgcggctcgg gccggggagg ccgccagtgc gtagccgctg
     4861 ttacgccata gccgcatcca cctcgatgcg cgagcgcgcg gcctacccgg gcaacttcat
     4921 cgggtcgatg ttcacctacg ccctgttcgt cttcatcttc tcgcgggtct gggcgggcgc
     4981 ctacgccggg gcctcggaga tcgcgggcta cacgatgcgc atggccgtct ggtatttcat
     5041 cgtcgcggag gtgccgagct tcggcttcgg ccgtttcttc tggaccctgt ccagggacat
     5101 gaagaacggc cagatcgcct acctgctcgc gaggccgtac gactacgtcg gatacaactt
     5161 cgccgagaag tatggcggct cgctggtcga ggccggcgtg atcctggtcg agggactgct
     5221 ggtcgggatc ctgctcgtcg gcccgccccc cctcgccggg ggcgcgccgt cgctcgccgg
     5281 gggcgcaggc gtcgccgcgg aggcctcgcg ggctctcctc gtcgggctat ccctgctgct
     5341 cgccggggcg ctcaactact tcctgcaggc gtccatcgcc atgacggcgt tctggctcga
     5401 ggagaacgag gccttcttct ggatcttcca gaaactcacc ctcgtcgtcg ggacgctcat
     5461 accgatcgag ttcctgccga cggccgcggc gaccctggcc aggctgacac ccttcccata
     5521 cctgtcgtac gcgccggcga ggatcatggt ggcgtactcg cccggcgagg cctggtcgct
     5581 cgtggccgcg caggcgctct ggctggtcct ggcggcggcc ctggccagga tcgtgttctc
     5641 gttcgggagc cgcaggctct cggtgaacgg aggctgacat gctagggacc ggacggaacg
     5701 gccccggccg cctcggcggc cgcactcgcg cgtacgcgcg cttcctcagg gacgccttcg
     5761 tcgtcaacct gaaggcaacg ctcgagtacc gcgtcaactt catcatgcag ttcttcggca
     5821 tgatgctcaa caacgccgcc ttcgcggcct tctggctggt gctcataggc cggaccggcg
     5881 ccgtcggcgg ctacggcttc tcggacgtga tgctgatctg ggccctcgtg tccacctcgt
     5941 tcggcctggc ccacgtcgtc ttcggcaacg tcaggcagct gggccgcatc atcatggagg
     6001 gcggcctcga cgtctacctc ctgcagccca aggatgcgtg gatcaacgtg ctcgcgtcga
     6061 ggaccgtcgt gtcggcttgg ggcgacctgg cctacggctt catcgtgctg gcgttgatga
     6121 acggtacgaa cctggagcgt ctcgcgttgt tcacggccct ggcagtcccg ggtgcgctga
     6181 tcttcgcggc cacgttcgcg gcggccgaga gcctgacctt cttcatgggc aactcgtccg
     6241 ccgtgtcatc cgccctgacc gagttcatgc tgtcgttctc gctgtacccg gagggcatct
     6301 acggccacgg aatgcgctgg atactgtact ccatcgtgcc gtcgggcttc gtggcattca
     6361 tgccgctacg cgtcttccgg tcgctggact ggggcctcgt gccactcctg tacctggtgg
     6421 cggcggccta cgtcgggctg tcgtactggt tgttcagggc gggcctcagg cgctacgagt
     6481 cgggcaaccg tatggacgcg cgggtctagg gtcgatagaa tccgtcgaaa cgcgagagga
     6541 acatccgatg ctcgtacaga aagcgcgggg gtcggcgcta cccgcggaga tctacgccct
     6601 gttcgccgta cggctggtcg tgtcggccgg ctcgttcgtc gggcccttcc tggcgatgat
     6661 gctgacgatg aagctgggct acgacgaggc tcgggccggc gccttcatgt cagtcgtgtc
     6721 ggtggcgtcg gccctcggcc tcgtcgtcgg cggcaagcta ggcgacgcct tcgaccggcc
     6781 tcgcgtactg cggatcctgc aggcgactac cgccgcggcc tacgtcgtct gcgccgtcat
     6841 gggcttcaac gccgccacgc ccttcgtcat agccttcgcg atgggcacgc tcaacggcac
     6901 ctggcccatc atcaacgcca tcgtcgccga cgccgcgccg gccgagcgcc gcaaggaagc
     6961 cttctcgctc ctgtactggg gtaataatat cggtttctcg atagggctgt tcatagctgg
     7021 cctgctgttc acgagggcgc ctcaggcgct gttcgccggc aacgcggccg tgctgttcgc
     7081 ggccgcgatc atcgtggccc gctacgtgcg cggcggcgcc cggaccgaag cgccggtggg
     7141 cgttccgacc atcgaccggc aggccaccgc agccgccgcg gccgacggcc cgatagcctg
     7201 cccggccgac gtgcagccga cgtgcagccg accgcggccg ccgcggccgc tccgtcgtcc
     7261 gtctgggtcg tgttcaggcg tagcccggtg ctgctgctgt actcggccgg atccgtgctg
     7321 acgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgtgttc aggcgtagcc
     7441 cggtgctgct gctgtactcg gccggatccg tgctgacggc cttcgtctac aatcagcata
     7501 ccttcgcgct accgatattc ctgaacgacg cgctcggcga ggtggccggg ccaagggtgt
     7561 tcggagccgc gatgatggcc aacggcctga ccgtcgtcgc gctgacggcg ctcgtcgtac
     7621 tggcttcgaa gcggcttcgc tcgctgactt ccgtggcgct ggcgtcggcg ttctacgcgg
     7681 tcggcttcgg ggcctattac ctggtcggcg gctcgcgcta cggcgtgccg ctcgccctcg
     7741 gcgcgaccgt cgtctggacg gtcggcgaga tactgggcag caccaatggc aacgccttcg
     7801 tcgccgagaa ggcgccgccc gcctaccgta gccgggtcaa ctcggtcgta tcgttctcct
     7861 atatcgccgg cggcgccttg gcgccgctcg cggccggctc gatcgcgcga gcctacggca
     7921 gcgcggccgt ctggcctttc gtggcggcca gcgccgccct gtccgcggcc ttcatgtacg
     7981 gcgtcgaccg gctggacaag aagaccggcg cgcgggtcac agcggcaggc tcctgaacag
     8041 gtaggcgatc gatacgccca ggtagtttcc ggctgcgtag ccgacgaggc ccgtggtcag
     8101 tccagtcatc agcacggccc gattcttaag cgcctcggcg agtaccggta cgaatggcgg
     8161 agagcatata gccgagaccg aggtgacgat gaaggtatcc acgtcgatgc cagccagctt
     8221 ggacaggagc gcgtgtacga ccaggctgcc gaagatcgag gcggacacgt agccgagcac
     8281 cgcccagtcg acgcgaacca ggctgtcgag cttcgtcatc gacgcgacga cgaagcagaa
     8341 tacgtagatg acgtacatgc cggcctggaa cgtatgcctg acgcgcctga ccggcccgat
     8401 aaacgacgcg gccagggaca gcgtggtgac ggccaggatg gttaccgcgg tgccgcccgt
     8461 gccaggcgcg agcgagccga ggaaggccga ggcgccgacg atggccgcgg acaggagcga
     8521 ggccaggacc agcccgcgga ccgcgccagg ctcccggaac gagatgccgg ccgcgtcgtt
     8581 agccatatcg ccgccagtct ccggagctcc cgccgccggc tcgaagccgc gcatccgcgc
     8641 gaccctcagc aggatgggcc gcgctaccga cgccatcacg acgatgtagg cgaacgaaat
     8701 gaccgcatcg taggtgtgga aaaggatata ggtatcgttt gcgacgccga tggccgcctt
     8761 gatggccgcc aggtttggcg ttccgcccgt gtacacgccg acggcgagcc cggccagttg
     8821 ccacgcgtcc ggcctcccgg agtggaacgt cagctgtagc gcgaaggtca ccgcgattat
     8881 ggacagggcg gcaagcgcca tgcacagcat gcccttgccc gccaccttga accatgccct
     8941 gacgtcgatg gagaacagga gcaggggcag ggcgagtacg acgctgacgt cggccatggc
     9001 agactggacg ggcgcgaaac cctccggtag gactccgacg ttgccgacca cgacgccggc
     9061 cgcgtagcat aggagcaccg cgcctatcgt gcggaggggc ttgaaatggc cgcataggta
     9121 gacgatcagc gcgggggcca ctaggaaggc cagggcgaac agcgcgttca tcgattcctc
     9181 ctcgtcggtc gggccgggtc ggtacagtgt agcccgacta tcgcgccggg tcgagggccg
     9241 cgcggctatc cgccgtcgag cggcccaccc cttgccgccg agtcccagcc gtgctaccaa
     9301 tggcccatgg acgataacgg gttctgcttt aggccgctcc gcgcctccga caaggcggcc
     9361 gtgatggcca tcagcgctaa gatctgggaa ggcgacgact acctgccgca ctcgttcgac
     9421 gactgggtct ccgaccccga cggcctgttc aacggctgct tcgtaggcga ccggctcgtc
     9481 ggcttcggcc gggtcggttt cgtcgcgccg ggccacgcct ggctcgaggg cctccggaag
     9541 gacccggacg ccggcgtctc gggcgtcggc cgggccgtag ccgtcgaggg actgcgtttc
     9601 ctgtcgggcg ttcacgatct ccgctcgata cgcttcagcg cctacgccca caatccgcag
     9661 acgatagccg tcaatgaggc gctcggcttc aggcgcgcgg gcgcctggtc gatcaagtcg
     9721 aagcgactgc ctggctcggc ggacggtaag gaggccttcg gcgccccggc cgccgcgccg
     9781 tcagaccgct ccggcgtatc cgtccgccgc gtcgacccgc tcgacgcctc ggccgccggg
     9841 cggattcgag ccgccctgac caccggaccc tgggccgccg gcttcaggct ggagtcgtgg
     9901 aaggcctacc cgctcgaggc gagcccggcg atcagggaag acgacctgat cctcgaggcg
     9961 gtcggtagcg atgggtccgt cctcggacgg ctcatcggct cgctcgatcc gctcaaggcc
    10021 caggccaatg tcgtcgcctt cggcttggag cctagctctc catcggacgc gtcctcggcc
    10081 tcgagcgacg acctagccgc cgccgcgctg ttcgcggcct tcgagcggat cgcctccgac
    10141 gccggctacg cgtacttcga ggccatcgcg ccgggcctcg caggccagcg cgaggccctc
    10201 gacgcctgcg gctatgaaag ctgggagagc gaggacgact tcctcctcta cgagtggccg
    10261 gccgagtggc tgggcgagct ggcgaggccg cgctaggccc cgcccgagaa gaagcgcccg
    10321 aggagctcgt agacctcggg gtaggcggcc tcgaggcgct gcggtgcatc gaagaaggcc
    10381 tcgcaggcga ccgcgaagaa ctcgtcgggg ctctcggccg cgtactcgtc gagcggcagt
    10441 ctcgaggctc ggcccacacg cctcccgctc gacgaacggg ccacccgcac ctggaaatcg
    10501 gcgtaggcgt cgccgaaggc ggcgcgccag gcgcgccggc tcatcgacct gggcaggggc
    10561 gggcagccgt ctaggcctcc gtcccgctcg tcgagcttgt gggccatctc gtgcaccacg
    10621 acgttgtagc cgtcgccgaa gcccgactcg cgcacgtccg gcaacgagaa cagcacgggg
    10681 ccgagctcgg tgaccctgcc ggacagcagg tcgtcgtact cggtcaccac gcctgcgcca
    10741 tcgacgctcg tcatcctatg gacgaagccg tccggggccg ccagtatcgt cgaccagtcg
    10801 tcgtaccatc cggcgccgag cctgagcacg ggcaggcagg cgaggccgac cgccgcggcg
    10861 acgcggtagc agtaggt
//
LOCUS       DAOPHV010000019        10725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_7527_length_10725_cov_4.455764, whole genome shotgun sequence.
ACCESSION   DAOPHV010000019 DAOPHV010000000
VERSION     DAOPHV010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10725
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7527_length_10725_cov_4.455764"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1361
                     /gene="lysA"
                     /locus_tag="PLQ29_01055"
     CDS             93..1361
                     /gene="lysA"
                     /locus_tag="PLQ29_01055"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPG85258.1"
                     /translation="MTMAHLSFDGCDAVALAKEYGTPLYVVSERMIRERLRELRDDFL
                     GRYPNTYALYASKALQTLDVLRIVASEGIGLDVVSGGELFAASKAGFPMDRVYFHGNS
                     KTDAEIRMAVEYGVGRVVVDNLHELETLNRAATALGRRARILFRVAPGVDSHTHEYIS
                     TGRLDSKFGIPLDRRVRDAYVGAALGMEGVELLGFHFHVGSQLLSNESHLKAIGVVCA
                     LMAELKADYGFETAELNLGGGYGVHYHDDERARPLSYFVDPMMAELTAACATANLRLP
                     RVVIEPGRWVVGEAGLTLYTVGSKKVIPEVKTYVGVDGGLPDNPRPALYGAVYEASVA
                     DRHDAPRDEVVTVAGKCCESGDILIRDVALQSPEPGDVLAVFTTGAYNHSMASNYNRL
                     PRPAMVLTDSGEHRLSVRRETFDDLIAREL"
     gene            1363..2379
                     /locus_tag="PLQ29_01060"
     CDS             1363..2379
                     /locus_tag="PLQ29_01060"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPG85259.1"
                     /translation="MRDEYADRGVPALHERLIVIDGHCDTAIPAAGYDFKSERVPPLD
                     LCARNDTGHVDFPRLLEGGVTAQFFALFTDTVFVPEAREHTYRLLDAVESAVGRSGGR
                     ARLATTSGEVLAAKRDGALAAFLTIEGGEAIGESLDELRAFHARGVRLMTLTWNRVNA
                     IGRGAEHPGPDGLTPFGREVVAEMENLGMIVDVSHLCDQALDEVLAVARRPLVASHSN
                     SRAVCDHRRNLSDAQAERIAATGGLIGVTFAGVFVDPDPAKVGVARVVDHIDRLVSVV
                     GVEHVGLGTDFDGFSAPYGLVMPDCSRLGQLTAALQARGYAEADVAAIMGGNWLRVIR
                     DVAG"
     gene            complement(2384..3250)
                     /gene="rlmJ"
                     /locus_tag="PLQ29_01065"
     CDS             complement(2384..3250)
                     /gene="rlmJ"
                     /locus_tag="PLQ29_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HPG85260.1"
                     /translation="MLSYRHGFHAGNHADVLKHTTLVACLRAMRAKETPLLYVDTHAG
                     AGSYALREGFAAQNDEWAGGVERLAAFAGNENPPPVVADYLAAVIGPGGAQAMERYPG
                     SPAIAAAWLRPQDRLALFELHPSDYGPLSAMFSGDRRARVVNADGFSGLRGALPPPSR
                     RALVLVDPSYELAADYERAIEALREALRRFATGVYAVWYPLLERPEAKALPRRLMDLA
                     AEAGPRGAAALDARLRVRGSRPGERGMAGSGLVVFNPPWRLADGLREALPYLARALGD
                     DADASWDLETRE"
     gene            3360..3887
                     /locus_tag="PLQ29_01070"
     CDS             3360..3887
                     /locus_tag="PLQ29_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85261.1"
                     /translation="MKRILSIICALALLTLPLAAAPAKSKPAKQASPTTIRLSGVPAD
                     GPMGLGLFLGQPTGVSFELDLGPASWLDAKAAWNFGGGHGGYSILLQANYEYAFPGML
                     VIEAASFTPFFGLGAFFNLYDGGFGLGARVPGGLAYRFRKAPFELFIEVGLDIPMFPA
                     FGIGASGGLGVRYRF"
     gene            3906..4670
                     /locus_tag="PLQ29_01075"
     CDS             3906..4670
                     /locus_tag="PLQ29_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HPG85262.1"
                     /translation="MATRSASFEALILRSKEVPSGARVATLLTADEGLVEAFVFGGGK
                     SKLRSLASPWHYGRAWIYRDAAKGLVKLSDFDPEREFASVRGSLEAIAAASFASELMI
                     ATSALGGDWPDALELTVAALAAFDEAASRGDQGLLGRAVSLFALRALDAMGLGPDSGE
                     CRSCAGVMGDDAIHSYSRRSGGFVCARCAGREPDAIPLPPGAVAWLGAAARRPFADAV
                     RVGLADEAARALKACALDLAEKAAETPLRTLKSGLV"
     gene            4681..6051
                     /locus_tag="PLQ29_01080"
     CDS             4681..6051
                     /locus_tag="PLQ29_01080"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HPG85263.1"
                     /translation="MRAVDVIMKKRDGGELSRDEIAAIVNGYVSGEVPEYQVSAWLMA
                     VFFRGMSFRETADLTDLMLRSGSRMDLSGIAGPFVDKHSTGGVGDKVSLILAPIAAAC
                     GVRVPMMSGRALGHTGGTLDKLESIPGYTTRLDEARFREYIERDGFAMTGQTDAIVPA
                     DKRLYSLRDVTATVESVPLITASILSKKVAEGAEALVFDVKSGPGAFMKTVDDAERLA
                     SSLVRTGQAMGKRIVGVITDMTEPLGDKVGNFLEVEESIDCLEGRGPADLMEVTYRLA
                     AWMLVAAGNDAAGNDAAGNDAAGNGAAGVAQDVEAATRLCEKAVASGKALELFMRNVR
                     TQGGDVDRMMKMRGSFRSRHVRELRAPAAGYVRSIDAYKIGLAGVYLGVGRNKTSDPV
                     APDVGFVFERRKGAAVAKGDLVATVYGKDEASLDAAWPLVAEALSVGPEAPAASPLVI
                     KEITAL"
     gene            6048..8168
                     /gene="recJ"
                     /locus_tag="PLQ29_01085"
     CDS             6048..8168
                     /gene="recJ"
                     /locus_tag="PLQ29_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPG85264.1"
                     /translation="MRWKKRDVSPQAIRELAERFGLDLLSASVLARRGVLSPEEVLYY
                     LEDDLRFQRNPFLFSQMEDAVDRILLAADEGEKVLVFGDRDADGVSATTLMMEALASL
                     GVDARYRLPTEDEKYGLSRAAVDEFAADYGSLIVTVDCGISNHAEMAYAAEKGVDVIV
                     VDHHVLQADEPPPALAVIDPKLESSGYPFRDLSGCGVAYKLAWALRFAKSGLYKQQVA
                     LLNVRPVKDAYVIEALRLSNLVETGRVVETIVPGMVELERTRLVPFLKDRQIFVWDGE
                     IQKRLLAKALGKAAEVNFYDVRPDVARVIPQAAGASLVRLAEMSKVGKYSDRPSGELD
                     AFANLFVSFALRKAGCQGESDADALQLVALSTIADLMPLRDENRLLVRQGLAALNRKP
                     RKGLAELVARQNLTGKRVGSTDAAWQLTPVINAAGRMGKPEVATRMLLSDDAAERGAQ
                     ADALLELNQERRRLGAECWDAVYPAAREAAEASGGKYVIIGRDDLFRGITGIVASRLA
                     DTFKAPAVAGTFMADGTAVASVRSARGFNVKGLLEYCSELFYDYGGHDAAAGFSLPVG
                     KWPDFERMAAEYLSAVDLEEVEETVEIDAELPHEYLAPSLAGLVERMEPFGEGNPALV
                     FMARSVAILQVDIVGKQEKSHLKLLLDFGAHRWPALWWNAAERYGKDFDESDRLDLAF
                     RLQKNYWNGQETPQLVIVDARKAE"
     gene            complement(8194..9597)
                     /locus_tag="PLQ29_01090"
     CDS             complement(8194..9597)
                     /locus_tag="PLQ29_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPG85265.1"
                     /translation="MTTRAITRPATLAAVSMACLLACLTAGATPAWGQTVAAITQIDG
                     ASLLARQRVRLYVAGIDDGADAVRVQESADGRNWEDRPVEAVTEAANADEGVTFFFLL
                     DDSGSMWSEAGVDEAGSRIGQAKNAARRFIARLGPADRLGLAVFNTRYWRAAEPTADR
                     AAVLAAIDEIARPSRDDAFTELYQSIDRAVLELAAYPGRKAIVVLSDGEDYPYAARTG
                     SPSPEFGEAAVPPDEAAERAIAEGVTCYAVRFGPDRDPDIGAMAERTGGAAFDAADGL
                     ELAEVYATIRNDLLREAAVDYRAGMAPGDRRYVRVSLDDGGNWSAPRYYYAGAVFGQA
                     RSAPGWLDLVPLLLAAAVWLAFWLLKLERPMDRAGLSLLYGPRDARTEVYALSGAKTV
                     IGGSADADITLAGNPAMAADHATILFDELRGEYTIAADAELRVNNRPVKRKRLEPGDV
                     IDMAGTVVVFDEPTKGK"
     gene            complement(9624..10517)
                     /locus_tag="PLQ29_01095"
     CDS             complement(9624..10517)
                     /locus_tag="PLQ29_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPG85266.1"
                     /translation="MSLTLKRLFMALFGLLGALVAWPALSALRFLQPRFPGYLVFTLA
                     QGAAAGLAFGAMLGSIEGVVVSSRAKALRGLGFGALYGALAGAAGAAAGQLAMFVAGE
                     TLFRSASARLGPALALSNGVAWAIIGLAIALTEGLRARSPRKLAAGLAGGTVGGLLGG
                     AVLSALSYARPDDGAALLAGLAIFGLAIGASYAVFENRFSFGALKLLNGPLKNKEYLL
                     ASRRLTIGSDDACDVVLKGYPGVAPLHAVIRAGKDGSRLEPGPDSPRVAVNDEPLGDR
                     PLRQEDVIAIGKAKFIYGYFG"
     gene            complement(10520..>10725)
                     /locus_tag="PLQ29_01100"
     CDS             complement(10520..>10725)
                     /locus_tag="PLQ29_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85267.1"
                     /translation="SLYRFGYWVPLSELPAGTLMTGSVHKFQIEAEGYEPSVYSLSIG
                     NGQDELRIEAALAPFGAAPSAKE"
BASE COUNT         1626 a         3796 c         3677 g         1626 t
ORIGIN      
        1 cgggcgcgcc gacgggccaa ggctccgtcg gcgctagccc tagcgcctcg gccgtggtac
       61 tctttcctgg ccgacaggcg tgaaaggggc gcatgaccat ggcgcatctg agcttcgacg
      121 gctgcgacgc cgtcgccctc gctaaggaat acgggacccc cctctacgtg gtctccgaac
      181 gcatgatacg cgagcgcctg cgcgagctgc gcgacgactt cctgggacgc tacccgaaca
      241 cctacgcgct gtacgcgagc aaggcgctcc agacgctcga cgtcctgcgc atcgtcgcct
      301 ccgagggcat aggcctcgac gtggtctccg gcggcgagct gttcgcggcc tccaaggccg
      361 gcttcccgat ggaccgggtc tatttccacg gcaactccaa gaccgacgcc gagatcagga
      421 tggccgtcga gtacggcgtc ggccgggtcg tcgtcgacaa cctgcacgag ctcgagacgc
      481 tgaaccgggc cgcgacggcc ctgggccggc gcgccaggat actgttccgc gtcgccccgg
      541 gcgtggatag ccacacccac gagtacatct cgaccggtcg gctcgactcc aagttcggca
      601 tcccgctcga ccggcgcgtc cgcgacgctt acgtcggcgc ggccctcggc atggagggcg
      661 tcgagctcct cggcttccat ttccacgtcg gctcccagct cctgtcgaac gaatcccacc
      721 tcaaggccat cggcgtcgtc tgcgcgctga tggccgagct caaggccgac tacggcttcg
      781 agacggccga gctgaacctc ggcggcggct acggcgtcca ctaccacgac gacgagcggg
      841 ccaggccctt gtcgtatttc gtcgacccga tgatggccga gctgaccgcc gcctgcgcga
      901 ccgccaacct gcgcctgccc cgcgtcgtga tcgagcccgg acgctgggtc gtcggcgagg
      961 ccggactcac cctgtacacc gtcggctcca agaaggtcat cccggaggtg aagacctacg
     1021 tcggcgtcga cggcggcctg cccgacaatc cgaggccggc cctatacggc gcggtctacg
     1081 aggcgagcgt ggccgaccgg cacgacgcgc cgcgcgacga ggtcgtcacg gtcgcgggca
     1141 agtgctgcga gtcgggcgac atcctgatac gcgacgtcgc gctccagtcg cccgagcccg
     1201 gcgacgtgct cgccgtgttc acgaccggcg cctacaacca ctcgatggcc agcaactaca
     1261 accgcctacc gcgcccggcg atggtgttga ccgattccgg cgagcaccgc ctgtcggtcc
     1321 ggcgcgagac cttcgacgac ctgatagcca gggagctgtg atatgcgcga cgaatacgcc
     1381 gatagaggag tccccgcgtt acacgaacgc ctgatcgtca tcgacgggca ctgcgacacg
     1441 gccataccgg ccgccggcta cgacttcaag agcgaacggg tgccgccgct cgacctctgc
     1501 gctcggaacg atacgggcca cgtcgacttt ccccggctgc tcgagggcgg cgtgacggcg
     1561 cagttcttcg ccctgttcac cgacacggtc ttcgtgcccg aggcccgcga gcatacctac
     1621 cggctcctgg acgcggtcga gtcggccgtc gggcggtccg ggggcagggc gcggctcgcg
     1681 acgacgtccg gggaggtcct cgccgccaag cgcgatggcg ccctggccgc cttcctgacc
     1741 atcgagggcg gcgaggcgat aggcgagagc ctcgacgagc tgcgcgcctt ccacgcgcgc
     1801 ggcgtgcgcc tgatgacgct gacctggaac cgcgtcaacg ccatcggccg cggcgccgag
     1861 caccccggcc ccgacggact gaccccgttc ggccgagagg tcgtcgccga gatggaaaac
     1921 ctcggcatga tagtcgacgt gtcgcacctc tgcgaccagg ccctcgacga ggtcctggcc
     1981 gtggcgcggc ggccgctcgt cgcgtcgcac tccaactcga gggccgtctg cgaccaccgg
     2041 cgcaacctga gcgacgccca ggccgagcgc atcgccgcga ccggcggcct tatcggcgtc
     2101 accttcgccg gcgtcttcgt cgatcccgac ccggccaagg tcggcgtcgc ccgggtggtc
     2161 gaccacatcg accgcctggt ctcggtcgtc ggcgtggagc acgtcggcct cggtaccgac
     2221 ttcgacggct tctcggcgcc ctacggcctc gtgatgcccg actgttcccg cctgggccag
     2281 ctgaccgcgg ctctccaggc ccgcggctac gccgaggccg acgtcgccgc gatcatgggc
     2341 ggcaactggc tgcgggtcat ccgcgacgta gccggctgat gcctcattcc ctcgtctcca
     2401 ggtcccatga ggcgtcggcg tcgtcgccga gggcgcgagc caggtagggc agggcctcgc
     2461 gcaggccgtc ggccaggcgc cacggcgggt tgaagacgac gagccctgag ccggccatgc
     2521 cgcgctcccc gggccggctt ccgcgtacgc gcagcctcgc gtcgagcgcg gccgccccgc
     2581 gcgggccggc ctcggcggct agatccatta agcgccgagg cagggccttg gcctccggcc
     2641 gctcgaggag cgggtaccag accgcgtaga cgcccgtggc gaagcggcgg agcgcctcgc
     2701 ggagcgcctc gatggcgcgc tcgtagtcgg ccgccagctc gtacgacggg tcgacgagca
     2761 cgagggcgcg ccgagacggc gggggtagcg cgccgcgcag tccggagaag ccgtccgcgt
     2821 tgaccacgcg cgctcggcgg tcacctgaga acatcgccga cagcggcccg tagtcgctcg
     2881 ggtgtagctc gaacaacgcc agccggtcct ggggccggag ccaggcggcc gctatggccg
     2941 gcgagccggg atagcgctcc atcgcctgag cgccgccggg gccgatcacc gctgccaggt
     3001 agtcggccac gaccggcggc gggttctcgt tcccggcgaa ggcggccagg cgctccacgc
     3061 ctccggccca ctcgtcgttc tgggcggcga agccttcccg cagcgcgtac gagccggctc
     3121 cggcatgcgt gtcgacgtag aggagcggcg tttccttggc gcgcatcgca cgcaggcagg
     3181 ctacgagcgt cgtatgcttg agcacgtcgg cgtggttgcc ggcgtggaag ccgtgtctat
     3241 agctgagcat cgcaggtcct ccgcgcggcg ccgcctggcc tcggagcggc cacggctcga
     3301 acaagccggc cggatatgct agcatactat cgacctagcg tcttccaagg agtcttcgca
     3361 tgaaacgcat cctgtctatc atctgcgccc tggctctcct gaccctgccg ctggctgccg
     3421 ctccggccaa gtcgaagccg gccaagcagg ctagccctac caccattcgc ctgtccggcg
     3481 taccggccga cggtccgatg ggcctgggcc tgtttctcgg ccagcctacc ggcgtcagct
     3541 tcgagctgga cctcgggccg gcttcctggc tcgacgctaa agccgcatgg aatttcggcg
     3601 gcgggcacgg cggctactcc atcctccttc aggccaatta cgagtacgcg ttccccggca
     3661 tgctcgtcat cgaggccgcc tccttcacgc cgttcttcgg cctcggagcc ttcttcaatc
     3721 tctacgacgg cggcttcggc ctcggagccc gcgtgcccgg cggcctagcc taccgcttca
     3781 ggaaggcccc gttcgagctg ttcatcgaag tcggcctcga tataccgatg ttcccggcct
     3841 tcggcatagg cgcgtccggc gggctcggcg tccgctaccg cttctagcgg gccgcgggcg
     3901 cggcggtggc gaccaggtcg gcctcgttcg aggcgctgat actgcgctcc aaggaagtgc
     3961 cgtccggggc gagggtcgcc acgctcctga ccgcggacga ggggctggtc gaggctttcg
     4021 tcttcggcgg cggcaagagc aagctgcgct cgctcgcctc gccctggcat tacggccgcg
     4081 cctggatcta ccgcgacgcg gccaagggcc tcgtcaagct aagcgacttc gacccggagc
     4141 gcgagttcgc gtccgtccgc ggctcgctcg aggccatcgc ggccgcgtcg ttcgcgagcg
     4201 agctcatgat cgcgacgagc gcgctcggcg gcgactggcc cgacgcgctc gagctgacgg
     4261 ttgccgcgct cgcggccttc gacgaggcgg cctcgcgcgg cgaccagggc ctgctcggcc
     4321 gggccgtcag tctctttgcc ctgcgggcgc tcgacgcgat ggggctcggc ccggattcgg
     4381 gggaatgccg ctcctgcgct ggcgtaatgg gcgacgatgc gatacactcc tactcccgcc
     4441 gatcgggagg cttcgtctgc gcgcgttgcg ccgggcggga gcccgacgcg atacccctgc
     4501 cgccgggcgc ggtcgcctgg ctcggggcgg cggcgcgcag gccgttcgcc gacgccgtac
     4561 gcgtcgggct ggcggacgag gcggcgcgcg cgctcaaggc ctgcgcgctc gacctggccg
     4621 agaaggcggc ggagacgccg ctacgtacct tgaaatccgg attagtgtaa ggaggccgtc
     4681 atgagggccg tagatgtcat tatgaagaag cgcgacggcg gcgagctgtc ccgcgacgag
     4741 atagccgcga tcgtgaacgg gtacgtctcg ggcgaggtgc ccgagtacca ggtgagcgcc
     4801 tggctgatgg ccgtgttctt ccggggcatg agcttccgcg agaccgccga cctgaccgac
     4861 ctgatgctgc gctccggctc gaggatggac ctgtccggca tcgccggccc cttcgtcgac
     4921 aagcactcga ccggcggcgt aggagacaag gtgtcgctga tactcgcgcc catcgcggcc
     4981 gcctgcggcg tcagggtgcc gatgatgtcg ggccgcgcgc tcgggcacac cggcggcact
     5041 ctcgacaagc tcgagagcat ccccggctat accaccaggc tcgacgaggc gcgcttccgc
     5101 gagtacatcg agcgcgacgg tttcgcgatg accggccaga ccgacgccat cgtgccggcc
     5161 gacaagcgcc tgtactcgct acgcgatgtg accgccaccg tcgagtcggt gccgctgatc
     5221 accgcgagca tcctgtccaa gaaggtggcc gagggcgccg aggccctcgt cttcgacgtc
     5281 aagagcggcc cgggcgcgtt catgaagacc gtggacgacg cggagcgcct ggcctccagc
     5341 ctcgtgcgca ccggtcaggc catgggcaag cgcatcgtcg gcgtgatcac cgacatgacc
     5401 gaaccgctcg gcgacaaggt cggtaacttc ctcgaggtcg aggagtcgat cgactgcctc
     5461 gagggcaggg ggccggccga cctcatggag gtgacctacc gcctggccgc ctggatgctc
     5521 gttgccgcag gcaacgatgc cgcaggcaac gatgccgcag gcaacgatgc cgcaggcaac
     5581 ggagccgcgg gcgtggccca ggacgtcgaa gccgcgacga ggctgtgcga gaaagccgtg
     5641 gcgtcgggca aggcgctcga gctgttcatg cgcaacgtaa ggacccaggg cggcgacgtc
     5701 gaccgcatga tgaagatgcg cggctcgttc cgctcccggc atgtccgcga gctgcgggcc
     5761 cccgccgccg gttatgtgcg ctcgatagac gcgtacaaga tcggcctggc cggcgtctac
     5821 ctcggcgtcg ggcgcaacaa gacgagcgac ccggtcgcgc cggatgtcgg cttcgtcttc
     5881 gagcgcagga agggcgccgc cgtcgcgaag ggcgacctgg tcgccacggt ctacggcaag
     5941 gacgaggcct cgctcgacgc ggcctggccc ctggtggccg aggctctgtc cgtcgggccc
     6001 gaagccccgg ccgcctcgcc cctcgtgata aaggaaatca ccgcgctatg aggtggaaga
     6061 agcgcgacgt ctccccgcaa gccatccgcg agctggcgga gcgcttcggc ctcgacctcc
     6121 tgtctgcctc cgtcctggcc aggcgcggcg tcctgtcgcc cgaggaggtg ctgtactacc
     6181 tcgaggacga cctgcgcttc cagcgcaatc ccttcctatt ctcgcagatg gaggacgcgg
     6241 tcgaccgtat cctgctcgcc gccgacgaag gcgagaaggt cctcgtcttc ggcgaccgcg
     6301 acgccgacgg cgtctccgct acgaccctga tgatggaggc cctcgcctcg ctcggtgtcg
     6361 acgcgcgtta ccgcctgccg accgaggacg agaagtacgg gctgtcgcgg gcggccgtcg
     6421 atgagttcgc cgccgactac ggcagcctca tcgtcaccgt cgactgcggc atatcgaacc
     6481 atgccgagat ggcctatgcc gccgagaagg gcgtcgacgt gatcgtcgtc gaccatcacg
     6541 tgctgcaggc cgacgagccg ccgccggcgc tggccgtcat cgaccccaag ctcgagtcgt
     6601 ccggctaccc cttccgcgac ctgtcgggtt gcggcgtagc ctataagctg gcctgggccc
     6661 tgcgtttcgc caagagcggc ctctataagc agcaggtcgc cctcctcaac gtcaggccgg
     6721 tcaaggacgc ctacgtgatc gaggcgctcc gcctgtccaa cctggtggaa accggccgcg
     6781 tggtcgagac catcgtgccg ggcatggtcg agctggagcg cacgaggctc gtgcccttcc
     6841 tcaaggaccg gcagatcttc gtctgggacg gggaaatcca gaagcgcctc ctggccaagg
     6901 ccctcggcaa ggcggccgag gttaacttct acgacgtcag gcccgacgtc gctcgcgtca
     6961 taccgcaggc cgccggggct agcctcgtcc gcctggccga gatgtccaag gtcggcaagt
     7021 actccgaccg gccgtctggc gagctcgacg ccttcgcgaa tctcttcgta tcgttcgcgc
     7081 tacgcaaggc cggctgccaa ggcgaaagcg acgccgacgc cctgcagctc gtcgccctgt
     7141 cgacgatagc cgacctgatg cctctgcgcg acgagaaccg gctcctggtg cgccagggac
     7201 tggccgccct gaatcgcaaa ccgcggaagg gcttggccga gctcgtcgcc aggcagaacc
     7261 tgaccggtaa gcgcgtcggc tcgaccgacg ccgcctggca gctgactccc gtgatcaacg
     7321 cggccgggcg catgggcaag ccggaggtgg ctacgaggat gctgctgtcc gacgacgcgg
     7381 ccgagcgagg ggcccaggcc gacgcgctgc tcgaattgaa ccaggagcgg cgaaggctcg
     7441 gcgccgagtg ctgggacgcc gtctacccgg ccgcccgcga ggccgccgag gcgtccggcg
     7501 gcaagtacgt gatcatcggc cgagacgacc tcttccgcgg catcaccggc atcgtcgcca
     7561 gccgcctggc cgacaccttc aaggcgccgg cggtcgccgg taccttcatg gccgacggca
     7621 cggccgtggc ctcggtgcgc agcgcccgcg gcttcaacgt gaagggcctc ctcgagtact
     7681 gctccgagct gttctacgac tacggcggcc acgacgcggc cgcgggcttc agcctgccgg
     7741 taggcaagtg gccggatttc gagcgtatgg ccgccgagta cctgtcggcg gtcgacctcg
     7801 aggaggtcga ggagaccgtc gaaatagacg ccgagctgcc gcacgagtac ctggcgccgt
     7861 cgttggccgg gctcgtcgag cgcatggagc cgttcggcga gggcaacccg gccctcgtat
     7921 tcatggccag gtcggtggcc atcctgcaag tcgacatagt cggcaagcag gaaaagagcc
     7981 atctcaagct gctcctcgac ttcggcgccc accgatggcc ggcgctctgg tggaacgccg
     8041 ccgagcgtta cggaaaggac ttcgacgagt cggaccggct cgacctggcc ttccgcctcc
     8101 agaagaacta ctggaacggc caggaaaccc cgcagctcgt tatcgttgac gccaggaagg
     8161 ccgagtaagc ggctcggtcc aggcgcccgc gcctcatttt cccttggtcg gctcgtcgaa
     8221 gaccaccacc gtccccgcca tgtcgatcac gtcgcccggt tccagccgct tgcgcttgac
     8281 cggccggttg ttgacgcgta gctcggcgtc ggcggcgatg gtgtactcgc cgcgaagctc
     8341 gtcgaacagg atggtcgcgt ggtcggcggc catcgcggga ttgcccgcca gggtgatgtc
     8401 ggcatcggcc gagccgccga tcaccgtctt ggcgcccgat agcgcataga cctcggtcct
     8461 ggcatcgcgc ggcccgtaga ggaggctgag gccagcccgg tccatgggac gctccaactt
     8521 gagtagccag aaggccaacc agacggccgc cgcgaggagc agcggcacca ggtcgagcca
     8581 gccgggcgcc gaacgtgcct gcccgaacac ggcgccggcg tagtagtagc gcggcgcgct
     8641 ccagttcccg ccatcgtcga ggctgacgcg cacgtagcgg cggtcgcctg gcgccatgcc
     8701 ggcccggtag tccacggccg cctccctgag caggtcgtta cggatcgtcg cgtagacctc
     8761 cgccagctcc aggccgtcgg ccgcgtcgaa ggccgcgccg ccggtccgtt cagccatggc
     8821 gcctatatcc gggtcgcggt cggggccgaa gcgcacggcg tagcaggtga cgccctcggc
     8881 tatggctcgc tccgccgcct cgtccggagg taccgccgct tcgccgaact cggggctcgg
     8941 gctgccggtc cgggccgcgt aggggtagtc ctcgccgtcc gacaggacga cgatggcctt
     9001 gcggcccggg taggcggcca actcgaggac ggcgcggtct atcgattggt acagctcggt
     9061 gaacgcgtcg tcgcgcgacg gtctggctat ctcgtcgatg gctgccaata cggcggcacg
     9121 gtcagccgtc ggctcggccg cgcgccaata cctggtgttg aacacggcta agcccagcct
     9181 gtcggccggc ccgagcctcg cgatgaagcg cctggccgcg ttcttggcct ggccgatgcg
     9241 cgagccggcc tcgtcgaccc cagcctcgga ccacatcgat cccgagtcgt ccagcaggaa
     9301 aaagaacgtg acgccctcgt cggcgttcgc cgcctcggtc accgcctcga cgggccggtc
     9361 ctcccagttc cggccgtcgg cggactcctg tacgcggacc gcgtcggccc cgtcgtcgat
     9421 cccggcgacg tatagcctga cgcgctgcct ggcgaggagg ctcgcgccgt ctatctgggt
     9481 tatcgcggct acggtctggc cccaggcggg cgtcgcgcca gccgttaggc acgccaatag
     9541 acaggccatc gagacggccg ccagcgtcgc tggtcgggta atcgctcgcg tagtcatggt
     9601 tcgctcctta tcgtcggccc gcttcatccg aagtacccgt agatgaattt ggccttaccg
     9661 atcgctatca cgtcttcctg gcgtagagga cggtcgccga ggggctcgtc gttgacggcg
     9721 acgcgcgggg agtccggccc cggttcgagg cgtgagccgt ccttgccggc gcggatcacc
     9781 gcgtggagcg gcgcgactcc cggatagccc ttgagcacga cgtcgcacgc atcgtccgag
     9841 ccaatcgtca gccgtcggct cgcgagcagg tattccttgt tcttgagggg gccgttgagc
     9901 agcttgagcg ccccgaagga gaaacggttc tcaaagacgg cgtaggacgc gcctatggcc
     9961 aggccgaaga tagccagccc ggcgagcagg gcggctccgt catcggggcg cgcgtacgat
    10021 agcgccgata ggacggcccc gccgagtagc ccacccacag tgccgccggc gagtccggcg
    10081 gccagtttgc ggggagatcg ggcgcgcagg ccctcggtca gcgctatggc caggccgatg
    10141 atggcccagg ccacgccgtt cgacagcgcc aaggccggcc cgagcctggc ggaggccgac
    10201 ctgaatagtg tctcgccggc aacgaacatg gccaactgcc cggcggcggc tcccgcggcg
    10261 ccggcgaggg cgccgtatag ggccccgaac ccgagtcccc gcagcgcctt ggcgcgcgac
    10321 gagacgacga cgccctcgat cgagccgagc atcgcgccga aagccagacc ggctgcggcg
    10381 ccctgcgcca acgtgaaaac caggtagccg gggaagcgcg gctgtaggaa cctgagcgcc
    10441 gacagggccg gccaggcgac cagcgcgccg agcaggccga acagggccat gaagagtcgc
    10501 ttaagcgtca gggacatgat tactccttgg cggacggcgc cgctccgaac ggggccaggg
    10561 ccgcctcgat tcgtagctcg tcctgcccgt tgcctatcga tagcgagtag accgacggtt
    10621 cgtagccctc ggcctctatc tggaatttat gtaccgatcc ggtcatcagc gtgccggccg
    10681 gcagctccga cagcgggacc cagtagccga aacggtacag cgata
//
LOCUS       DAOPHV010000020        10094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_8199_length_10094_cov_4.211575, whole genome shotgun sequence.
ACCESSION   DAOPHV010000020 DAOPHV010000000
VERSION     DAOPHV010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 10094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10094
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8199_length_10094_cov_4.211575"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PLQ29_01105"
     CDS             complement(<1..734)
                     /locus_tag="PLQ29_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPG85268.1"
                     /translation="MTIIQAAILGLFQGIAEFLPISSSGHLLIFKDLMGLAEVPALFD
                     VVLHVATLASIVVVFRRRIGGIIASMARWVARRHGEGDAENLAIVIPAIVATALTGAV
                     GLAIDRIDFSGRPEVASGLLLVTALILIAGSRLKPGATTYRGMTVRHGVIVGLAQGIG
                     VLPGISRSGISISAGLASGLERGTAGEFSFLLAIPAILGALVLKLDELPAMAGSVAVP
                     QLVVGSVVAFAAGVGALLALMPLVRKG"
     gene            986..1531
                     /locus_tag="PLQ29_01110"
     CDS             986..1531
                     /locus_tag="PLQ29_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPG85269.1"
                     /translation="MEIQKDRVVSIDYMLKDDQGQIIDRSEGEPLVYLHGNDNLIPGL
                     ERELHGKVAGDKISCVIAPADAYGELDEEMIFKVAKGDFAEPDKIEVGMQFQAQDASG
                     AHIVTVVGIEGDEVKIDANHPLAGKSLHFDVTVKDVREALPEELEHGHVHADGECGCG
                     DGCCGDGDCGDEGGCGCGGCH"
     gene            1522..1932
                     /locus_tag="PLQ29_01115"
     CDS             1522..1932
                     /locus_tag="PLQ29_01115"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HPG85270.1"
                     /translation="MPLIRRAGATRGRGGFQVRWLDALIVALSAALFLYLAVSVYGSP
                     PSSVLVITSGEHEWIYQLTQEREIDVPGLIGMSVIGIHDGAAHIDDSPCANRTCVASQ
                     PISRAGEWAACLPNGVFIRIEGEEHIDEIDATVR"
     gene            1907..2491
                     /locus_tag="PLQ29_01120"
     CDS             1907..2491
                     /locus_tag="PLQ29_01120"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HPG85271.1"
                     /translation="MRSTRPYARPTFADGGRRLAAFLAALAFFLSTVEYMLPRPVPFM
                     RLGLANLPLLIAVDLLPFGSYIALALVKVVGMSVLTGSLFSYVALFSLAGTLASALVM
                     RGLRAVAGPRSLSYVGLCVAGAVSSNLAQVALARVFVFGDSARFMAPAFVGLGLVSGL
                     ALGAFTQAFASKSAWLAGVLAPPVVADASAGGQA"
     gene            2488..3126
                     /locus_tag="PLQ29_01125"
     CDS             2488..3126
                     /locus_tag="PLQ29_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85272.1"
                     /translation="MRRPAAAARAERREHWEGAFDPTRLALTGVLLSIVLLFQPTLYG
                     RVAMLAAAAAAAWASGRRLSPLMTVFVMAGIVAANLLVPMGRRLATFGPLVVTETALL
                     TGIEKAVTFEALMFISKACLGPALRLPGRFGSFFADALRGYDRILEHKGSVKLDGFMR
                     RIDEILISVYDGFQSGGDRVDTGYRGRPAAFRRGDPALAVIALAAVVSLLIR"
     gene            3181..5310
                     /locus_tag="PLQ29_01130"
     CDS             3181..5310
                     /locus_tag="PLQ29_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG85273.1"
                     /translation="MALSLRSKLLVLIAVPLAMVAAMGAMLILSSMGDKGEIDDAESN
                     LTIMKSLSSLLSAAQDERGIAYLYLSGTAAADRIMSTRNGTGSSWETVQAALAAVPAP
                     ESLETVLTSSVARFQQLRRQVDERSMMAQSAYTGYSELIDLVMAEYVALSATNAAEAS
                     RQLQSIVYLEKAKENAARIQCLASSIFSLDLAIQYTKTIELVDTNAGIAVNLANAGEY
                     CGELASVSLSELEISPEWYELQDAVLSILGNAQYGGYGRDGLALYNATEPVMDKIQAT
                     IDIVMAETDAWFAERSALLTRRIVLIAGALGAAALVVLVVSLLILSNVASRMKEVSEG
                     MRNVASGDADLTLRVASRTQDELGALAGFFNGFAEKLAGIIDRVKAEVGSLGDGMVQL
                     ASNTEETAGAIRQITANIESLKLQSINQSASVTESSATVEQIARNIEQLYRLIERQSE
                     GVSSSSSSIEQMVASIQSVTANIERMGSYYDKLLGKSDAGRGAIATVVAQVREIDSQS
                     ENLQAANQLIAGIAAETNLLAMNAAIEAAHAGEAGMGFAVVADEIRKLAENAAVQSKT
                     IGQTIKGIRGVIEAVVASSGQSASTFEEILEQIRILSRLEEEVKYSMQEQSAGSSQIL
                     ESLASINEVTADVRHSAQEMQEGSNAVLTEMRQLLQLASELENGMSEMASGAEEIRRA
                     AQDTNELTIAASASVKNASAEVAQFKT"
     gene            5338..6627
                     /locus_tag="PLQ29_01135"
     CDS             5338..6627
                     /locus_tag="PLQ29_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HPG85274.1"
                     /translation="MRTLARWLAAALALASATPIAAQPAPLGAAPAIAPAASSPSSSP
                     ASSPYKVTLITILPGKALYSSFGHSGLRVVDARSGRDLFYNYGLSAHPFDAKFALGMF
                     AGRMEFMVGAGATSSVLGFYSGVENRTIIEQDLALDATQAAALVAALRRDARPENRIY
                     NYRYFSDNCTTRVAVMLQRTAGEDPGSRPVDAALTVRASVNGTLAGRPWLIFVINLLM
                     GPTVDRQAGTGDPIFLPGQLMDWAAAAVNGGAAEPSERSRPFVSATRTLYEAEPTTER
                     AGLGPTAFAALLAAASLALTTLRGRLKPLAAAFDAACAASALVVGAAILLFWLCARYD
                     EAAWNLNLLWAGALPLVATILGGRRLPAKARSAAGALYAVSAACAALVAVAGGFGFQT
                     ILPATRLVALALALRWADRAGALGAAARLLRRRSRPE"
     gene            complement(6720..9158)
                     /gene="leuS"
                     /locus_tag="PLQ29_01140"
     CDS             complement(6720..9158)
                     /gene="leuS"
                     /locus_tag="PLQ29_01140"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPG85275.1"
                     /translation="MAKYPFSEIEPKWQRHWEEHRSFRAEEDASFPKEKRRYVLDMFP
                     YPSGAGLHVGHPEGYTATDIYCRYLRMNGYNVLHPMGFDSFGLPAENYAIQTGTHPAV
                     TTRANIDRFRQQIKSLGFSYDWDREVSTCEADYYKWTQWIFIQLYQRGLAYESDAPIN
                     WCPSCKTGLANEEVKDGECDRCGTKVTRKRIRQWMLRITAYADRLLEDLDGLDWPEPV
                     KQMQRNWIGRSEGASVVFTVDGHGDALEVFTTRPDTLFGATYMVLAPEHALVARITSA
                     EQKAAVEAYVDGAAKKSDLERTDLAKDKTGVFTGAYAINPVNGKKIPIWISDYVLASY
                     GTGAIMAVPAHDERDWDFAKAFGLPIVEVVAKPGPDGKAAPCAGEPSECTAADGVAVN
                     SGFLDGLGTEAAKKAMIAWLEEKGLGKGTINYKLRDWLFSRQRYWGEPIPLVHCPKCG
                     VVPVPEDQLPLTLPEVSSYEPSGTGESPLAKIDDWVDTTCPTCGGPARRETNTMPQWA
                     GSCWYYLRYLDPSNPGAFVGRDKVDYWMPVDLYVGGVEHAVLHLLYSRFWHKVLFDLG
                     LVNTAEPFQRLVNQGMILGEDNQKMSKSRGNVINPDDIVREYGADALRLYEMFMGPLE
                     VSKPWMTAGLVGVTRFLERMYALGEKPVVDAGPSAELLKLTHKTVRKVDADTASLNFN
                     TAISAMMVLSSELARLDSLPRAAYEPLVLMLAPYAPHLAEELWEKLGHRESLVRAAWP
                     SYDPALCEDDEDTIVVQVNGKLRGDFKAAKGAGKDALEASAKALAKVAEQLAGKDVVK
                     LVVVPGKLVNFVVR"
     gene            complement(9300..9758)
                     /locus_tag="PLQ29_01145"
     CDS             complement(9300..9758)
                     /locus_tag="PLQ29_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85276.1"
                     /translation="MNANGTNGLSPAVRRRRFAVRVGLVALYFVVGAWVMLSGREHTL
                     LLDNKALADGSAPAFRRVKVYVDGRDPIELYARDRDMIKVRGQRHRIRIETNEAAART
                     EASFTLAFGDDMFLLSVPAMAKGDDGFHEVFRTAEDRPAPEAEATPEPTF"
     gene            complement(9755..>10094)
                     /locus_tag="PLQ29_01150"
     CDS             complement(9755..>10094)
                     /locus_tag="PLQ29_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG85277.1"
                     /translation="MGNMATYNAHRQTGFFAVAAILVGGASVTKATIPNVFVGVVLLH
                     LMYIVMPRAGAELFGSAQIGEYFRDAFSYGAIALALVLHAWRKRRGVEIDRAGLRRNT
                     VSPDAKGGKA"
BASE COUNT         1653 a         3401 c         3335 g         1705 t
ORIGIN      
        1 cccttcctga ccagcggcat gagcgcgagg agggccccga cgccggcggc gaaagcgacc
       61 accgaaccga cgaccagctg cgggaccgcg acgctgcccg ccatggccgg cagttcatcg
      121 agtttgagca cgagggcgcc gagtatggcc ggtatggcca acaagaagga gaattcgccg
      181 gccgttcctc gctcgagccc ggaggcgagg ccggcgctga tcgatatgcc cgatcggctg
      241 atgcccggta gcacaccgat gccctgggcc aggccgacga taacgccgtg ccggacggtc
      301 atgccgcggt aggtcgtggc gccgggcttg aggcgcgagc ccgctatcag gatcaacgcg
      361 gtgaccaaca gcaggcccga ggcgacctcg gggcggcccg agaagtctat ccggtcgatc
      421 gccagcccga ccgcgccggt cagcgccgtc gcgacgatgg ccgggatgac gatggccagg
      481 ttctccgcgt cgccctcgcc atgcctcctg gctacccagc gcgccatcga tgcgatgatg
      541 ccgccgatgc gccgacggaa gaccacgacg atcgaggcga gcgtcgcgac gtgcaagacg
      601 acgtcgaaca gggccggcac ctcggcgagg cccatgaggt ccttgaagat gagcagatgc
      661 ccgctcgacg aaatgggaag gaattcggcg attccctgga aaagcccaag aatagcggcc
      721 tggatgatgg tcatgtcgcc ctccgggagc ggactatagc accatagcga cctaccttca
      781 acgccatccg ccctgtatgt agggtacggt acttgaaaat ctccattcca ataagtaccc
      841 tatgttagac gaaaaggcgt ttctttacgg acgtgatatc ctgggcttcc aggcagggct
      901 tagccgctct agacgcatca tggttcgcat cgttccgttc ggccccgccc gtcgacacac
      961 atactctctc tgtaaggatg gctacatgga aatccagaag gatcgcgtcg tctcgataga
     1021 ctacatgctc aaggacgacc agggacagat catcgaccgc tcggaaggcg agccgctcgt
     1081 ctacctccac ggcaacgaca acctgatccc cggcctcgag cgcgagctcc acggcaaggt
     1141 cgccggagac aagatcagct gcgtcatcgc tccggccgac gcctacggcg agctcgacga
     1201 ggagatgatc ttcaaggtcg ccaagggcga cttcgccgag cccgacaaga tcgaggtcgg
     1261 catgcagttc caggctcagg acgccagtgg cgcccacatc gtcacggtgg tcggcatcga
     1321 gggcgacgag gtcaagatcg acgccaacca cccgctcgcc ggcaagtccc tacacttcga
     1381 cgtgacggtc aaggacgtgc gcgaggcact acccgaggag ctcgagcacg gtcacgtgca
     1441 cgccgatggc gaatgcggtt gcggcgacgg ttgctgcggg gatggcgact gcggcgacga
     1501 aggcggctgc ggctgcggcg gatgccactg atccgacggg ccggcgcgac gaggggccgc
     1561 ggagggttcc aggtcaggtg gctcgacgcg ctgatcgtgg cgctctcggc ggccctgttt
     1621 ctatacctgg cggtctccgt ctacggctcg ccgccctcgt ccgtcctggt catcacctcg
     1681 ggcgagcatg aatggatcta tcagctgacc caggagaggg agatagacgt cccgggcctg
     1741 atcggcatgt cggtgatcgg catccatgac ggtgcggccc atatcgacga ctctccatgt
     1801 gcgaaccgga cctgcgtcgc ctcccagccg attagccggg caggcgaatg ggccgcctgc
     1861 ctacccaacg gcgttttcat ccgaatcgag ggcgaggagc atattgatga gatcgacgcg
     1921 accgtacgct aggccgacct tcgctgacgg cggtcgcagg ctagccgcct tcctggccgc
     1981 cctggccttc ttcctgtcca cggtggagta catgctgcct cggcccgtgc ccttcatgcg
     2041 gctcgggctg gccaacctgc cgctcctgat cgccgtcgac ctcctgccct tcggctcgta
     2101 tatcgcgctg gccctcgtca aggtcgtcgg gatgagcgtg ctgaccggat cgctattctc
     2161 ctacgtggcg ctgttctccc tggccggcac gctcgcgagc gcgctcgtga tgcgcggcct
     2221 cagggccgtg gccgggcccc gctcgctgtc gtacgtcggc ctctgcgtcg ctggagcggt
     2281 atcgtcgaac ctggcccagg tcgccctggc cagggtgttc gtcttcggcg acagcgccag
     2341 gttcatggcg ccggccttcg tcgggctcgg actcgtctcg ggcctggccc tcggtgcctt
     2401 cacgcaggct ttcgcgtcca agtcggcttg gctggcgggc gtgctcgccc cgcccgtcgt
     2461 cgcggacgcc tccgccggag gccaggcatg aggcggccgg cggcggccgc ccgggccgag
     2521 cggcgcgagc attgggaagg ggccttcgac ccgacgaggc tggcgctgac cggagtactg
     2581 ctatcgattg tgctcctgtt ccaaccgacc ctctatggcc gcgtcgcgat gctcgccgcg
     2641 gccgcggcgg cggcttgggc ctcgggccgc aggctatcgc cgctcatgac cgtcttcgtc
     2701 atggccggca tcgtagccgc caacctgctc gtaccgatgg gcaggcgcct cgctactttc
     2761 ggccccctcg tcgtgacgga gacggccctg ctcaccggca tcgagaaggc cgtgaccttc
     2821 gaggcgctga tgttcatctc caaagcctgc ctcgggccgg cgctcaggct gcccggacgc
     2881 ttcggctcgt tcttcgcgga tgccctgcgc ggctacgacc gcatcctcga gcataagggc
     2941 tcggtcaagc tcgatggctt tatgcggagg atcgacgaga tactgatttc ggtctatgat
     3001 gggttccagt ccggtggcga tcgagtcgat actggataca ggggacgtcc ggccgccttc
     3061 aggcgcggtg acccggccct ggccgtcatc gcgctcgccg cggtggtttc gttgctcatc
     3121 aggtaaaaag ccggcccgat agctcagcgg gcaacatcca ggtataccga ggttcgtagt
     3181 atggctcttt ctctgagaag caagctgctc gtactcattg cggttccgct ggccatggtc
     3241 gcggcgatgg gcgcgatgct catactatcg tcgatgggcg acaagggcga gatcgacgac
     3301 gccgagtcga acctgaccat catgaagtcg ctgagctccc tgttgtcggc ggctcaggac
     3361 gagcgcggca tcgcgtacct ctacctttcg ggcaccgccg ccgcggatag gatcatgtcg
     3421 acgaggaacg gcacgggaag ctcctgggaa acggtccagg cggcgctcgc cgccgttcca
     3481 gcaccggaaa gcctcgagac ggtcctgacg tcgtccgtgg cgcgtttcca gcagctgcgc
     3541 aggcaggtcg acgagcgctc gatgatggcc cagtcggcct ataccggcta ctcggagctc
     3601 atcgacctgg tcatggcgga gtacgtggcg ctttcggcca ccaacgccgc ggaggcctcc
     3661 cggcagctcc agtccatcgt ctacctcgag aaggccaagg agaacgccgc ccgtatccag
     3721 tgcctggcca gctccatctt ctcgctcgac ctggccatcc agtacaccaa gaccatcgag
     3781 ctggtcgaca cgaacgccgg tatcgccgtg aacctggcca acgccgggga gtactgcggc
     3841 gagctggcct ccgtgtccct atcggagctg gagatcagcc ccgagtggta cgaactccag
     3901 gacgccgtgc tgtccatcct cggtaacgcc cagtacggcg gctacggccg cgacggcctc
     3961 gcgttgtaca acgcgaccga gccggtcatg gataagatcc aggcgaccat agacatcgtg
     4021 atggccgaga cggacgcctg gttcgccgag cgctcagccc tgctgacgag gcgcatcgtg
     4081 ctgatcgccg gcgcgctcgg cgcggccgcg ctggtcgtgc tggtcgtatc gttgctgatc
     4141 ctgtcgaacg tggcctccag gatgaaggaa gtctcggagg gcatgcggaa cgtggcctcg
     4201 ggcgacgccg acctgaccct acgggtcgcg tccaggaccc aggacgagct cggagcgctg
     4261 gccggcttct tcaacggctt cgccgagaag ctcgcgggca tcatcgaccg ggtcaaggcc
     4321 gaggtcggct cgctcggcga tggcatggtc caactcgcct cgaataccga ggagaccgcc
     4381 ggcgccatca ggcagatcac cgcgaacatc gagagcctca agctgcagag catcaaccag
     4441 agcgcgtcgg tcacggagtc gagcgcgacc gtcgagcaga tagccaggaa catcgagcag
     4501 ctgtaccgac tcatcgagcg gcaatcggag ggcgtatcgt cgtcgtccag ctccatcgaa
     4561 cagatggtcg ccagcatcca gtcggtcacc gcgaacatcg agcgcatggg ctcgtactac
     4621 gacaagctgc tcggaaaatc cgacgccggc cgcggcgcca tcgcgacggt cgtcgcgcag
     4681 gtccgcgaga tcgactcgca gtcggagaac ctgcaggcgg caaaccagct gatagccggc
     4741 atcgcggccg agacgaacct gctcgccatg aacgccgcca tcgaggcggc gcacgccggc
     4801 gaggcgggca tgggcttcgc ggtcgtcgcc gacgagatac gcaagctcgc cgagaacgcc
     4861 gccgtgcagt ccaagaccat cggccagacc atcaagggca tccgcggcgt catcgaggcc
     4921 gtggtcgcca gctccggcca gagcgcttcg accttcgagg agatcctgga gcagatccgt
     4981 atactctcga ggctcgagga ggaggtcaag tactcgatgc aggagcagag cgcgggctcg
     5041 tctcaaatac tcgagtcctt ggccagcatc aacgaggtga ccgccgacgt aaggcactcg
     5101 gcccaggaga tgcaggaagg ctcgaacgcg gtgctgaccg agatgcgcca gcttttacag
     5161 ctggccagcg agcttgagaa cggcatgagc gagatggcct ccggggccga ggagatacgc
     5221 cgcgctgccc aggacacgaa cgaactgacg atagccgcgt cggccagcgt caagaacgcc
     5281 tccgcagagg tcgcccagtt caagacctga taggcttcgc cgttgtatac tcctcccatg
     5341 agaaccctag ctcgatggct cgcggcggcg ctcgcgctgg cctcggccac gccgatcgcg
     5401 gcccagcccg ctccactagg cgcagcgccg gccatagcgc cggcagcttc gtcgccctcc
     5461 tcgtcgcccg cctcgtcgcc gtacaaggta accctgatca ccatcctgcc cggcaaggcc
     5521 ctctactcgt ccttcgggca ttcggggctc cgcgtcgtcg acgcgcgtag cgggcgggac
     5581 ctgttctata actacggcct ctcggcccac cctttcgacg ccaagttcgc gctcgggatg
     5641 ttcgcggggc gcatggagtt catggtcggg gccggtgcca cctcgagcgt gctcggcttc
     5701 tatagcggcg tcgagaaccg taccatcatc gagcaggacc tggccctcga cgcgacgcag
     5761 gcggctgcgc tggtggccgc gctcaggcgc gacgccaggc ccgagaaccg catatataac
     5821 taccgctact tcagcgacaa ctgcacgacc cgcgtcgcgg tgatgcttca gaggacggcc
     5881 ggcgaggatc ccggctccag gccggtcgac gcggcgctga cagtccgggc ctcggtcaac
     5941 ggcacgctcg ccggcaggcc ctggctgatc ttcgtcatca acctgctcat gggcccgacg
     6001 gtggatcgcc aggccgggac cggcgacccg atcttcctgc ccggccagct gatggactgg
     6061 gcggccgcgg cggtcaacgg cggagcggcc gagccatcgg aacgctcgcg gcccttcgtc
     6121 tcggcgacga ggaccctgta cgaggccgaa ccgacaaccg agcgcgccgg cctcggcccg
     6181 accgccttcg ccgcgctgct cgccgccgcc tcgttagccc ttaccaccct ccgcggccgg
     6241 ctcaagcctc tcgccgcggc cttcgacgcg gcctgcgccg cgtcggcgct cgtcgtcgga
     6301 gccgctatcc tcctcttctg gctgtgcgcc cgctacgacg aggccgcctg gaacctcaac
     6361 ctgctctggg cgggcgcctt accactcgtc gcgacgatac tcggcggccg acgcctgcct
     6421 gccaaggcgc gatccgcggc cggagcgctc tacgcggtct cggcggcctg cgccgcgctc
     6481 gtcgcagtcg ccggcggctt cgggttccag actatcctac cagcgacgcg cctcgtcgcg
     6541 ctcgctctgg ccctgcgatg ggccgaccgg gccggcgctc tcggcgcggc ggcgaggctc
     6601 ctgcgtcgtc gatcccggcc ggaataataa aggccgcggg taacgactac ccgcggccca
     6661 tcgtcgctcg ataccggtag gcgcgccccg aaccggcgcg ctcccggact ggcgcccgcc
     6721 tacctgacca cgaaattgac cagcttaccg ggcacgacga cgagcttgac gacatccttg
     6781 ccggccagtt gctcggccac cttggcgaga gccttggcgg aggcttcaag cgcgtccttg
     6841 cccgcgccct tggcggcctt gaagtcgccg cgcagcttgc cgttgacctg cacgacgatc
     6901 gtatcctcgt cgtcctcgca gagggccggg tcgtagctcg gccaagcggc cctgacgagg
     6961 ctctcacggt gcccgagctt ttcccagagc tcctcggcga ggtgcggagc gtagggggcg
     7021 agcatgagca cgaggggttc gtaggccgcc cgtggcaacg agtcgagcct ggcgagctcg
     7081 gaggacagca ccatcatcgc ggagatggcc gtgttgaagt tcaggctggc ggtatcagcg
     7141 tcgaccttgc gcacggtttt atgcgtcagc ttgagtagct cggctgacgg ccccgcgtcg
     7201 acgaccggct tctcgccgag cgcgtacatc cgctcgagaa agcgcgtcac gccgacgagg
     7261 ccggccgtca tccagggctt tgacacctca agcggcccca tgaacatctc gtagagcctg
     7321 agcgcgtcgg cgccgtactc ccgcacgatg tcgtcgggat tgatcacgtt gccacggctc
     7381 ttggacatct tctggttgtc ctcgcccagg atcatgccct gattgaccag gcgctggaac
     7441 ggctcggccg tgttgacgag ccccaggtcg aagaggacct tgtgccagaa gcgcgagtag
     7501 agcagatgga gcaccgcgtg ctcgacaccg ccgacataga ggtcgacggg catccagtag
     7561 tcgaccttgt cgcggccgac gaaggcgccc gggttcgacg gatccaggta acgcaggtaa
     7621 taccagcagg aaccggccca ctggggcatc gtgttggtct cgcgcctggc cggaccgccg
     7681 caggtagggc aggtcgtatc gacccagtcg tcgatcttgg ccaacgggct ttcgccggtc
     7741 cccgacggct cgtagctcga gacctccggc agggtcagcg gcagctggtc ctcgggcacg
     7801 ggtacgacgc cgcacttcgg gcaatggacc agggggatgg gctcgcccca gtaacgctgg
     7861 cggctgaaga gccagtcgcg cagcttgtaa ttgatcgtgc ccttgcccag gcccttctcc
     7921 tcgagccagg ctatcatcgc cttcttggcc gcctcggtgc cgaggccgtc caggaagccg
     7981 gaattgaccg cgacgccgtc ggccgccgta cactccgatg gctcgcccgc gcacggagcc
     8041 gccttgccgt cggggccggg tttggcgacc acctcgacga tgggcaggcc gaaagccttg
     8101 gcgaagtccc agtcgcgctc gtcgtgggcc ggcacggcca tgatggcgcc ggtgccgtag
     8161 gaggcgagca cgtagtcgga gatccatatc ggtatcttct tgccgttgac cgggttgatc
     8221 gcgtaggcgc cggtgaagac gccggtcttg tccttggcca ggtcggttcg ctcgaggtcg
     8281 ctcttcttgg cggcgccgtc gacgtaggcc tcgaccgcgg ccttctgctc ggcgctcgtg
     8341 atcctggcga cgagggcgtg ctcgggagcg agcaccatgt aggtggcgcc gaacagcgtg
     8401 tcgggcctgg tcgtgaacac ctcgagcgcg tcgccgtggc catcgaccgt gaagacgacg
     8461 ctcgcgccct cggagcggcc aatccagttg cgctgcatct gcttgaccgg ctccggccag
     8521 tcgagcccgt cgaggtcctc gaggagacgg tcggcgtacg cggtgatgcg tagcatccac
     8581 tgcctgatgc gcttacgcgt caccttggtg ccgcagcggt cgcactcgcc gtccttgacc
     8641 tcctcgttcg ccaggccggt cttgcaggac gggcaccagt tgatcggggc atcggactca
     8701 taggccaggc cgcgctgata cagctggatg aagatccact gggtccattt gtagtagtcg
     8761 gcctcgcagg tggacacctc gcggtcccag tcgtaggaga aaccgagact cttgatctgt
     8821 tgccggaagc ggtcgatgtt ggccctggtc gtcacggcgg gatgggtacc ggtctggatg
     8881 gcgtagttct cggcgggcaa gccgaacgag tcgaagccca tcgggtgcaa cacgttgtag
     8941 ccattcatgc gcaggtagcg gcagtagata tcggtggccg tgtagccttc ggggtggccg
     9001 acatgcaggc cggcgccgga cgggtacggg aacatgtcga gcacatagcg ccgcttctcc
     9061 ttggggaagg acgcgtcctc ctcggccctg aagctgcgat gctcctccca gtgcctctgc
     9121 cattttggct cgatctcgct gaacgggtat ttagccatgc gtgtcgctcc ggtgctagaa
     9181 tgcgatgagg cggacccgca taggaccgcc tcgtcgtcag gttggctgta tactatcgca
     9241 atacgtagaa acgagacaag cgcggagccg cggcgaccgc gcccgccctt gcgagcctcc
     9301 tagaaggtcg gttcgggcgt cgcctccgcc tccggagccg gcctatcctc ggccgtcctg
     9361 aagacctcgt ggaagccgtc gtcgcccttg gccatggccg gcacggacag gaggaacata
     9421 tcgtcgccga aggccagcgt gaagctcgcc tccgtacgcg ccgcggcctc gttcgtctct
     9481 atccgtatcc tgtggcgctg gccgcgcacc ttgatcatgt cgcggtcgcg cgcgtagagc
     9541 tctatcgggt cccgcccgtc gacgtagacc ttgacgcgcc tgaacgccgg cgccgagccg
     9601 tcggccaggg ccttgttgtc caggagcagt gtatgctcgc ggccgctgag catcacccag
     9661 gctccgacga cgaagtacag cgcgaccagg ccgacgcgta cggcgaagcg cctgcgcctg
     9721 acggccggcg atagaccgtt ggttccgttg gcgttcatgc cttgcctccc ttggcgtccg
     9781 ggctgaccgt gttccggcgc aggcccgccc ggtcgatctc gacgccgcgg cgcttgcgcc
     9841 aggcgtgcag tacgagggcc agcgctatcg cgccgtagct gaacgcgtcg cggaagtact
     9901 cgcctatctg ggccgagccg aacagctcgg cgcccgcgcg cggcatcacg atgtacatca
     9961 ggtgcaggag cacgacgccg acgaacacgt tcgggatcgt agccttggtc accgaggcgc
    10021 cgccaacgag gatggccgcc acggcgaaga agccggtctg ccggtgcgcg ttgtaggtgg
    10081 ccatgttgcc catg
//
LOCUS       DAOPHV010000021         9748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_8629_length_9748_cov_4.031569, whole genome shotgun sequence.
ACCESSION   DAOPHV010000021 DAOPHV010000000
VERSION     DAOPHV010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9748
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8629_length_9748_cov_4.031569"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="PLQ29_01155"
     CDS             complement(<1..834)
                     /locus_tag="PLQ29_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPG85278.1"
                     /translation="MFDDMSPDDLEYVGKKAGRPDMLEKLTGEATFVSDMSLPGMLHV
                     QLKKSPYARARIKGVDVSKAAAMPGVRAVVTGDELDYHIGLYIVDKYILAKGEVRHYG
                     EAVAAVAAETIEQARAAVEAIEVDYEPLAPVLHHMDALKPDAPLVHPDLGTYSYIEAV
                     FTPKPGTNIANHTKLRKGDVEKGFAESTYVVEREYSCPSVQHVPMETHVAIVQWGRGD
                     KVSIWTSAQSPFTVRNLFCHAFGLPHINVRVQIPYVGGGFGGKAGIGVEPLCAVLSRK
                     AG"
     gene            complement(827..1312)
                     /locus_tag="PLQ29_01160"
     CDS             complement(827..1312)
                     /locus_tag="PLQ29_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPG85279.1"
                     /translation="MRIKLTVNGLIYERDCIEHKTLLRFLREDLGLMGTKEGCGMGEC
                     GACTVIMNGRAVNSCMVLAVEADGASIRTIEGEAEGPSLSDIQRAFERNHAVQCGFCT
                     AGMVMSVKELLAEHPKPSADQVKEAIEGNFCRCTGYTQIVEAVLDLTGSPEKKGELEH
                     V"
     gene            complement(1314..2126)
                     /locus_tag="PLQ29_01165"
     CDS             complement(1314..2126)
                     /locus_tag="PLQ29_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPG85280.1"
                     /translation="MVYEFRYLAPRTAAELAAVLDGHKEDGKLLAGGTDLIPGIRNGV
                     MKPKVVIDLKKYSGADRLSYGADGLSIGPAVTINDILRCKEARERYPVLCDCAHELAS
                     HQIRNRATVAGNVVNASPCSDMAPALLCLGAKATIRSATGERVVPFSQFFAGVKKTVL
                     APGEFVSCIMVPAEAAGARGRYLKLKRIAGHDLGIVGVLMTRRDGELRFAVSSAAPTP
                     VLVDSVTEKDSADEAVRKVLAAVSPISDVRGTKEYREHMIGVYARRLFTEVE"
     gene            2332..3285
                     /locus_tag="PLQ29_01170"
     CDS             2332..3285
                     /locus_tag="PLQ29_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPG85281.1"
                     /translation="MVSFPIDTEGGPQVVLELIYRLKVKDVMMRDVITASASSSLRDI
                     QLAMRDRGITGVPIVDGDRLLGIVSIGDVIQALDGGWIHELASERMSTSLIVLEDDMP
                     LSFAITYFNRYGYRRFPVLDKNASLVGIVTAADVLRALLIEMNREVEKLEEGLAGRAA
                     AARDAAATATAPGGEAGLTMSFVSRRFDFENAGKASAELKKALRGLGVDAASIRRAAI
                     VSYELEMNQVIHSDGGVMEFCAAQDSVRIVARDEGPGIADVEAALTEGYSTANEWIRS
                     LGFGAGLGLPNAKRSSDEFELSSRVGAGTTVLAVVKYQERS"
     gene            3282..3614
                     /locus_tag="PLQ29_01175"
     CDS             3282..3614
                     /locus_tag="PLQ29_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85282.1"
                     /translation="MKVSDLSTRLGFEAIYAPAGDADVNDGYASDLLSDVMAKAGDGS
                     VLITIQAHKNTVAVASLIGIPAVIICNDRPVPSDMIEAAEAEGVALLRTPKTQFEVAG
                     TLYPELRG"
     gene            3635..4369
                     /locus_tag="PLQ29_01180"
     CDS             3635..4369
                     /locus_tag="PLQ29_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPG85283.1"
                     /translation="MFVACDFHNHSCLSPCGSLEQSPALLARLARERGLGLVALTDHN
                     SALNAPAFAECAAREGVAALYGMEATSAEEVHVLCLFAEVGAALDFGRFIEARLPRLP
                     YDPGKLGDQVVVDADENVLDLPELYFGAALELDYGELCESAAERGAIVIPAHIDRPMF
                     GAISQLGFLPDGPYAAVEAVRPLPDGAARGYTVITGSDAHYPEHVARRPFGLELADGW
                     ASGGKVDLEAVRVALAEGRVRLPFAT"
     gene            4366..7503
                     /locus_tag="PLQ29_01185"
     CDS             4366..7503
                     /locus_tag="PLQ29_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG85284.1"
                     /translation="MRPTAVMARARGGAVAGIAAALLSLAPIVGRAAALGAVDLDDAT
                     VLAAPPPRVAGSEADYTRVLVDDEASLSDRLLVHVEGPKGLPLTAFTAEPDRTLFSRL
                     PESGRRGVWNALREEPLASSTLWLCFRLDNRSPCAAWALVVEGAGFKPLAMVIVGGDG
                     GIQHYGGEYLDALRMGTSLADLSAYALNVPPESERTVYLRLSEGSLFGPGFGVWSHGL
                     FLERSEARLGLMSGIAGFAVAACVFYLLLSSRGWPVPYSGGVRRADQALSYAMPTAAC
                     GSLLLSLTGGGVRHALALILGAAAAAMECVLAARRISTSPLDDGLGARRVAAAIATRA
                     LAALAFAAVIAFGSQRLASLALPTWLALAVSLAAGERLSAIATRSRASHAESKRLLAR
                     LESETRGGVDFMTATAAALRGPLHGMMGMLEDLEALVQDAPLPSRAASDLAIARAEAA
                     RLDILVSNILSYSGLGPNRLADENLDLASVARSAASLLRVSIAGKGVRVAVDAPVVEI
                     RGDIGVAHRLLYTAMSHASRITGASLVRVSASSDESRVRVYVEDDGGALDSPSGTVVD
                     KGQERIELDIDAVVLGRLAGLLGGTYRFERQSGMNVRVFDLPRLAPPGPADSRPRAMS
                     EFPSSDDDGSGGPARPAGRVLVAGNEPVALLSIKRRLEASGWSVHATVSSADALDRAL
                     GRPPARAAAAQPYDIVIIDSAMPGMSGFEFCESVRRSKSPEALPIVVLLEPGRVDEVE
                     RAFRAGANDYIVRPASGLELAARVKTLVDLSSSVKRELRQATRLAEFDKYRTLAMLSA
                     GVAHEINTPNNAVLRTVPMLKEIWSGIEPAVERLSLEEGGFSLGGFDYDDLRRDVPDM
                     LNDLYMGAQDIKKIVEGLKDYARAPSESASLAVVDANEAIRYAARLLKHTIAVSTDRF
                     SMTLSENLPKVKADRLKLTQVLVNVLENALQAISSRSDGVSVVSEPIDDLELGRSVAI
                     RIADEGAGMDPDVLASVCSPFFTTERDRGGSGLGMSVALGLVQDMRGTIDIQSERGAG
                     TAVTIRIPVWLGAEG"
     gene            7509..8915
                     /locus_tag="PLQ29_01190"
     CDS             7509..8915
                     /locus_tag="PLQ29_01190"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPG85285.1"
                     /translation="MDEKRGSVIVVDDDVGVLRRLDRLLRFEGYGEVIMCERADDAWP
                     ALRASRPIAMILDLMMPGVDGYATLERMREERPDVPVIVATAIDDLDTVVRCMKAGAF
                     DYVPKTAETSRLLASLEHAAMIGRLLEDNKALRDSLLHDDKSSIGFFEGIVTRDKKMS
                     DIFRYLAAIAKSDKPILICGESGTGKELFASAAHAASGRSGELVTVNIAGLDDAMFSD
                     TLSGHKRGAFTGADTDRLGLIEQAKGGTLFLDEIGDLPQQSQVKLLRLIEQRRYYPLG
                     ADMTRSSDALIVAATNRDLERACAAGEFRPDLYFRLETHLIRLPPLRERRDDLPLLVE
                     VFVARAAEKFGKPAPGVPIELYDLLGAYDFPGNVRELESMVHDAVGRADGRMLSLDSF
                     RRRVFKPGARPVAASPVAKNVFSTLATLPTLREASDALIAEALARSGGNQGVAAGLLG
                     LSRTALNRRIKLRIEEDA"
     gene            9443..>9748
                     /locus_tag="PLQ29_01195"
     CDS             9443..>9748
                     /locus_tag="PLQ29_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HPG85286.1"
                     /translation="MSDRVYSAPEVSPARYPLGEEIANAITHGVWALLSIAGLVVLIV
                     SAATRGDAWYVVSFAIFGASAVVLFTMSTIYHAVAAPKAKKVLRVIDHSSVYLLIAGT
                     "
BASE COUNT         1590 a         3196 c         3221 g         1741 t
ORIGIN      
        1 gcccgccttg cgcgacagta cagcgcagag tggctcgacg ccgatgccgg ccttgccgcc
       61 gaagccgccg ccgacgtagg ggatctgcac gcgcacgttg atgtgcggta gcccgaaggc
      121 gtggcagaac aggttccgca cggtgaacgg cgactgggcg ctcgtccaga tcgatacctt
      181 gtctccccgg ccccactgca cgatggctac gtgcgtctcc atcggcacgt gctgcaccga
      241 cgggcaggaa tactccctct cgacgacgta ggtcgactcg gcgaagccct tctcgacgtc
      301 gcctttgcgc agcttggtat ggttggcgat gttcgtgccc ggcttgggcg tgaagacggc
      361 ctcgatgtac gagtacgtgc ccaggtccgg atggaccagc ggcgcgtccg gcttgagcgc
      421 gtccatgtgg tggagcaccg gggccagggg ctcgtagtcg acctcgatcg cctcgaccgc
      481 cgcccgggcc tgctcgatgg tctcggcggc gacggccgcc acggcctcgc cgtagtgcct
      541 gacctcgccc ttggccagga tatacttgtc gacgatatac aggccgatgt ggtagtcgag
      601 ctcgtcgccg gtgacgacgg cccggacgcc cggcatcgcc gcggccttgg agacgtctac
      661 gcccttgatg cgcgcgcgcg cgtaggggct cttcttgagc tgcacgtgga gcatacccgg
      721 cagagacatg tcgctgacga aggtggcctc cccggtcagc ttctcgagca tgtccggccg
      781 tccggccttc ttgccgacgt actcgaggtc gtcggggctc atatcgtcaa acatgctcga
      841 gctctccctt cttctcgggg ctacccgtaa ggtcgagcac cgcctcgacg atctgggtat
      901 aaccggtaca ccggcagaag ttgccctcga tggcctcctt gacctggtcg gcgctcggct
      961 tcgggtgctc ggcgagcaac tccttgaccg acataaccat gccggccgtg cagaagccgc
     1021 actggacggc gtggttacgc tcgaaagccc gctgtatatc ggagagcgac ggtccctcgg
     1081 cctcgccttc tatcgtccgt atcgacgccc cgtccgcctc gacggcgagc accatgcagg
     1141 agttgaccgc tcggccgttc atgatgacgg tgcaggcgcc gcactcgccc atgccgcagc
     1201 cttccttggt gcccatgaga ccgagatcct cgcggaggaa acggagcagc gttttgtgct
     1261 ctatgcagtc gcgctcgtag atgagcccgt tgaccgtaag cttgattctc atatcactca
     1321 acctccgtga acaggcgccg ggcatagacg cctatcatgt gctcgcggta ctccttggtg
     1381 ccgcgaacgt cgctgatcgg cgatacagcg gcgagcacct tgcgtacggc ctcgtccgcc
     1441 gagtctttct ccgtgaccga gtcgacgagg accggcgtcg gggcggccga gctgacggcg
     1501 aagcgtagct cgccgtcgcg cctggtcata aggacgccga cgatgccgag gtcgtggccg
     1561 gcgatgcgct tgagcttgag atagcggccg cgagccccgg cggcttctgc cggaaccatg
     1621 atgcacgata cgaattcgcc cggcgcgagc acggtcttct tgacgcccgc gaagaactgc
     1681 gagaatggca cgacgcgctc gccggtagcc gagcgtatcg tcgccttggc tcccaggcag
     1741 agcagggcgg gagccatgtc ggagcatggc gaggcgttga ccacattacc agccacggta
     1801 gcgcggttgc gtatctggtg gctggcgagc tcgtgagcgc agtcgcatag aaccggatag
     1861 cgctcgcgcg cttccttgca gcgcaggatg tcgttgatgg tgaccgccgg gccgatcgat
     1921 aagccgtcgg ctccgtacga cagcctgtcg gcgccgctat acttcttaag atcgatgacg
     1981 accttcggtt tcatgacgcc gttacggata ccggggatga ggtcggtccc ccccgccaag
     2041 agcttgccgt cttccttatg gccatcgagc accgcggcca gttccgccgc cgtcctaggg
     2101 gccaagtacc tgaactcgta gaccatgtgt gctgcctttc gagcgtattc acgaaacaaa
     2161 aacccggatc atacgaaccg ggcttctaga aggaaatgct actgactcga ctaaagttaa
     2221 gacaccgatc cagcgaggtg tcaaggaaaa tgtactccgg gcggcggacc ggccgcctag
     2281 gcggctcgtg tcggcttgtg gtcgaaacgg tttcatgaga caatcgggcc gatggtctcg
     2341 ttccctatcg ataccgaggg cggcccccag gtcgtcctgg agctcatcta tcgcctcaag
     2401 gtcaaggacg tgatgatgcg cgacgtcatc accgcctccg cctcgtcgag cctgcgcgac
     2461 atccagctag ccatgcggga ccgcggcatc acgggggtgc ccatcgtcga cggcgacagg
     2521 ctgctcggca tcgtgtccat cggggacgtc atccaggccc tcgacggcgg ctggatacac
     2581 gagctcgcgt ccgagcgcat gagcacctcg ctcatcgtgc tcgaggacga tatgccgctg
     2641 tcgttcgcca ttacctactt caaccgctac ggctaccggc gctttcccgt actcgataag
     2701 aacgcctcgc tcgtcggcat cgtcaccgcg gcggatgtgc tgcgcgcgct gctcatagag
     2761 atgaatcgcg aggtcgagaa gctcgaggag gggctggccg gccgggcggc ggcggcgagg
     2821 gacgccgccg ctacggcgac ggcccccggg ggcgaggccg gcctgacgat gagcttcgtc
     2881 tcgcggcgct tcgatttcga gaacgccggc aaggcctccg ccgagctcaa gaaggcgctc
     2941 agggggctcg gcgtcgatgc cgcgtcgata cgacgggccg ccatcgtcag ctacgagctg
     3001 gagatgaacc aggtcatcca ttccgacggc ggcgtcatgg aattctgcgc ggcgcaggac
     3061 tcggtcagga tagtggcacg ggatgaggga ccggggatag cggacgtcga ggccgccctg
     3121 accgagggct actccacggc caacgagtgg atacgttcgc tcggcttcgg cgcgggcctg
     3181 ggcctgccga acgccaagcg atcatcggac gaattcgagc tttcatcgag ggttggcgcg
     3241 gggacgaccg tgctcgccgt cgtcaagtat caggagcgat catgaaggta agcgatctat
     3301 cgacgcgcct cggtttcgag gccatctacg cgccggccgg ggatgccgac gtcaatgacg
     3361 gctacgcgtc cgacctcttg tcggacgtca tggccaaggc tggggacggc tcggtgctca
     3421 tcaccataca ggcgcataag aataccgtcg cggtcgcctc gttgattggc atcccggccg
     3481 tcatcatctg caacgaccgt ccggtgccga gcgacatgat cgaggccgcc gaggccgagg
     3541 gcgtcgccct gctcaggacg cccaagacgc agttcgaggt cgccgggacg ctctatccgg
     3601 agctgcgggg ctaggttcgg cccggccccc cggcatgttc gtcgcctgcg acttccataa
     3661 ccactcgtgc ctatcgccgt gcggtagcct ggagcagtcg ccggcgctac tggccaggct
     3721 ggcccgcgag cgcggcctgg gcctcgtcgc gctgaccgac cataacagcg ccttgaacgc
     3781 tccggccttc gccgagtgcg ccgccaggga aggcgtcgcc gcgctctacg gcatggaggc
     3841 cacgagcgcc gaggaggtgc atgtgctctg cctgttcgcc gaggtgggag ccgccctcga
     3901 tttcgggcgc ttcatcgagg cgcgcttgcc gcgtttgccc tatgatccgg gtaagctagg
     3961 cgaccaggtg gtggtcgatg cggatgagaa cgtactcgac ctgcccgagt tgtacttcgg
     4021 cgcggccctc gagctcgatt atggcgagct gtgcgagtcg gccgccgagc gaggcgccat
     4081 cgtcatcccc gcgcatatcg accgccctat gttcggcgcc atatcgcagc tcggcttcct
     4141 gcccgacggc ccgtacgcgg cggtcgaggc ggtcaggcca ctgcccgacg gggcggccag
     4201 aggctacacg gtcataaccg gctcggacgc ccattatcca gagcacgtgg cgcggcgccc
     4261 cttcgggctc gagctggccg acggctgggc ctccggcggc aaggtcgacc tcgaggccgt
     4321 ccgcgtcgcg ctggccgagg ggcgggtgcg cctgccgttc gccacatgag gccgacggcg
     4381 gtgatggcgc gcgcacgggg cggcgcggtc gcggggatcg cggccgcgct cctctcgctc
     4441 gcgcctatcg tcggccgtgc tgccgcgctc ggcgccgtcg acctcgacga cgctacggtc
     4501 ctggccgctc ccccgccgcg cgtcgcgggc agcgaggccg actatacccg cgtcctggtc
     4561 gacgacgagg cctcgctgtc ggatcgcctg ctggtgcacg tcgaggggcc gaagggcctg
     4621 cccctgaccg cgttcacggc cgaacctgat cggacgctgt tctcgcggct gcccgagagc
     4681 ggtaggcgag gcgtctggaa cgccctacgc gaggagccgc tcgcctcgtc gactctgtgg
     4741 ctatgcttca ggctcgataa tcgctcgccg tgcgcggcgt gggccctcgt cgtcgaaggc
     4801 gccggcttca agcctctggc gatggtcatc gtcgggggcg acggcggtat ccagcattac
     4861 ggcggcgagt acctcgacgc cctgcgcatg gggacgagtc tggccgacct gtcggcgtac
     4921 gcgctaaacg taccgccgga atcggagcgg accgtctacc tgcgcctgtc cgagggctcg
     4981 ctcttcggcc ccggcttcgg cgtatggtcg cacgggctct tcctggagcg gtccgaggcc
     5041 aggctcggcc tgatgagcgg catagccggc ttcgccgtag ccgcctgcgt gttctacctg
     5101 ctcttgtcgt cgcgcggctg gcccgttcca tattccggtg gcgtccgtcg cgccgaccag
     5161 gcgctctcct acgccatgcc gaccgccgcc tgcggctcgc tgctgctcag cctgaccggc
     5221 ggcggggttc ggcatgccct ggccctgata ctcggcgcgg cggccgcggc gatggagtgc
     5281 gtcctggcgg cgagaaggat ttccacttcg cctctggacg acgggctcgg cgctcgtcga
     5341 gtcgcggcgg ccatagcgac cagggcgctt gccgccctag ccttcgcggc cgtcatagcc
     5401 ttcggatcgc aacgactggc ctcgctggcc cttccgactt ggttggcgct tgccgtcagc
     5461 ctggcggccg gcgagaggct gtcggccatc gccacgcggt cccgcgcctc gcacgccgag
     5521 tcgaagcggc tcctggcgag gctggaatcg gagacgaggg gcggcgtcga cttcatgacg
     5581 gcgacggcgg ccgcgctacg tggacccctc cacggtatga tgggcatgct cgaggatctt
     5641 gaagccctgg tccaggacgc tccgttaccg tcccgggccg catcggacct agccatcgct
     5701 cgagccgagg cggctcgcct cgatatactc gtatcgaata tcctcagcta ctcgggtctg
     5761 ggcccgaaca ggctggcgga cgagaacctc gacttggctt ccgtggctcg gtccgcggcg
     5821 agtctccttc gcgtttccat cgccggcaaa ggcgtccgag tcgccgtcga cgcgcccgtc
     5881 gtggagatac gcggagacat aggcgtggcc caccgcctgc tctatacggc gatgagtcac
     5941 gcctcgagga taaccggcgc ctcgctggtc agggtctcgg cttcttccga cgagagtcgg
     6001 gtacgggtct acgtggagga cgacggcgga gcgctcgact cgccatcggg gaccgtcgtc
     6061 gataagggcc aagagcgcat cgagctcgat atcgacgcgg tcgtgttggg ccggctcgcg
     6121 ggcctgctcg gcggcaccta ccgcttcgag cggcaatccg gcatgaacgt acgcgtgttc
     6181 gacctgcccc gcctcgctcc gcccggcccc gcggactcga ggccgcgcgc catgtcggag
     6241 ttcccatcct cggacgacga cgggtcgggc gggccggccc gtccggcggg gcgggtgctc
     6301 gtcgcgggga acgaaccggt cgctctcctg tcgatcaagc gtcggctcga ggcctcgggc
     6361 tggtcggtgc acgctaccgt gtcatccgcc gacgcgctcg atagggcgct cggaaggcct
     6421 ccggctcggg ccgccgccgc ccagccctac gacatcgtca tcatagattc ggccatgccg
     6481 ggcatgagcg gcttcgaatt ctgcgagtcg gtgcgccgca gcaagtcgcc cgaggcgctc
     6541 cccatcgtcg tcctactcga gccgggtcgg gtcgacgagg tggagcgggc cttccgcgcc
     6601 ggcgcgaacg actatatcgt tcgcccggcc tcgggcctcg aactggccgc cagggtcaag
     6661 acccttgtcg acctgtcgtc gagcgtcaag cgcgagctgc ggcaggccac ccgcctggcc
     6721 gaattcgaca agtaccgaac cctggctatg ctgtcggccg gcgtggcgca cgagatcaat
     6781 acgccgaata acgcggtcct gcgcaccgta cccatgctca aggagatatg gagcggcatc
     6841 gagccggccg tcgagcgtct gagcctcgag gagggcggct ttagcctggg cggcttcgac
     6901 tacgacgacc tccggcgcga tgtgcccgac atgctcaacg acctgtatat gggcgcgcag
     6961 gatattaaaa agatagtcga gggcctcaag gattacgcca gggccccgtc agagagcgcc
     7021 agcctggcgg tcgtcgacgc caacgaggcc attcgctacg ccgcgcgcct gctcaagcat
     7081 acgatagccg tatccaccga tcgcttctcg atgacgctgt ccgagaacct gcccaaggtc
     7141 aaggcggaca ggctcaagct gacccaagtg ctcgtcaacg tgctcgagaa cgccctccag
     7201 gccatctcga gcaggtcgga cggcgtctcg gtggtcagcg aaccgatcga cgacctcgag
     7261 ctcggccgct ccgtagccat ccgcatcgcc gacgagggcg ccggcatgga cccggatgtc
     7321 ctggcgtcgg tatgctcccc gttcttcacc acggagcgcg atcgtggcgg ctcggggctc
     7381 ggtatgtccg tcgccctcgg cctggtccaa gacatgcgcg gcaccatcga tatacaatcg
     7441 gagcgcgggg ccggtaccgc cgtgacgata cgcatcccgg tatggctcgg cgcggagggc
     7501 tgatcatcat ggacgagaaa cgcggttcgg tcatcgtcgt cgacgacgac gtcggagtgc
     7561 tgaggcggct ggaccgcctg ctgcgcttcg aaggctacgg agaggtgatc atgtgcgagc
     7621 gggccgacga cgcgtggccg gcgctgcgcg cgtcccggcc gatagccatg atcctcgacc
     7681 tgatgatgcc cggagtcgac ggctacgcga cgctcgagcg gatgagggag gagcggcccg
     7741 acgtaccggt catcgtcgcc acggcgatcg acgacctgga taccgtcgtc cgatgcatga
     7801 aggcgggcgc cttcgactac gtaccgaaga cggccgagac ctcgaggctc ctggcgagcc
     7861 tcgagcatgc cgcgatgata ggcaggctgc tcgaggacaa taaagccctg cgcgacagcc
     7921 tgctccacga cgacaagagc tccatcggct tcttcgaggg catcgtcacg cgcgacaaga
     7981 agatgagcga catcttcagg tacctggcgg ctatcgccaa gagcgacaag cccatcctga
     8041 tctgtggaga gtcgggcacc ggcaaggagt tgttcgcctc ggcggcccac gccgcctcgg
     8101 gacgatcggg agagctcgta acggtcaata tcgccggcct cgacgacgcg atgttcagcg
     8161 atacgctgtc tgggcataag cggggggcct ttaccggagc cgataccgac cggctcggcc
     8221 tgatcgagca ggccaagggc ggcacgctgt tcctcgacga gataggcgac cttccacagc
     8281 agagccaggt caagctactc aggttgatcg agcagcgccg atactaccct ctcggcgctg
     8341 acatgacacg ctcgtccgat gctctgatcg tcgcggcgac gaaccgcgat ctcgagcggg
     8401 cctgcgccgc gggcgagttt aggccggatc tatacttcag gctggagacg cacctgatac
     8461 ggttgccgcc gctccgcgag cgccgcgacg acctgccgct actggtcgaa gtcttcgtcg
     8521 ccagggccgc cgagaaattc gggaagcctg cgccgggcgt gccgatagaa ctctacgact
     8581 tgctcggcgc ctacgatttc cccggtaacg tgcgcgagct cgagtcgatg gtgcacgacg
     8641 ctgtcggacg cgccgacggc agaatgctgt cgctcgattc gttccgtcgc cgggtgttca
     8701 agccgggcgc gcgcccggtc gcggcgtctc ccgtggccaa gaacgtattc tctacgctgg
     8761 ctaccctccc gaccctgcga gaggcgtccg acgcgctgat agcggaagcc ctggcgcgct
     8821 cgggcggcaa tcagggcgta gccgccggtc tcttgggcct gtccaggacg gccttgaacc
     8881 ggcgcatcaa gcttcgtatc gaggaagacg cttaaggcgg attccgggtt gcccgctacg
     8941 tctcagccag tacctatcgc cgccgatgat ggggatggcc atgcaagaag ggtgtgcaaa
     9001 attcgacacg aggcgctttt ggtacatgct gcttatatca ttttctggaa agaagatagc
     9061 tattgatata gcttcttggc agacttgtag tggcaagtag tcgacaacat tgtcgtttcg
     9121 gcgatggttg tcgagccgca taaatcatta atatataaat atatacattg cgttcactcc
     9181 cttgtgatct tggcacggta attgctttat accatgtggt tggttgagcc ccaagtcagg
     9241 taaaaaatgg tatgtaaaca agtgatgtaa acaagtcgcg gtaaacaatg ggggtaatgt
     9301 aaacatcgtg aatccgagat agcggaacgt cagggccgac gttccgcttt ttttgcgtcc
     9361 ggcccggcgc aacctcgccg ggtggccgcg gtcgaacatg gcaaggaagc ggcgctcgac
     9421 gccgccctcg aaaggtcgtt cgatgtcaga tcgcgtttac tccgctccgg aagtctcccc
     9481 cgcccgctat cctctcggcg aggaaattgc caacgccata acgcacggcg tctgggccct
     9541 tctgtctatc gccggccttg tcgtgctgat cgtatcggcc gcgacgcgtg gggacgcatg
     9601 gtacgtcgtt tcgttcgcaa tattcggtgc tagcgccgtc gtgctattca ccatgtcgac
     9661 gatctaccac gcggtcgcag ccccgaaggc caagaaagtc ctccgggtca tcgaccactc
     9721 gtcggtctat ctcctgatag ccggtacc
//
LOCUS       DAOPHV010000022         9710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_8683_length_9710_cov_4.632729, whole genome shotgun sequence.
ACCESSION   DAOPHV010000022 DAOPHV010000000
VERSION     DAOPHV010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9710
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8683_length_9710_cov_4.632729"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1494)
                     /gene="hisE"
                     /locus_tag="PLQ29_01200"
     CDS             complement(139..1494)
                     /gene="hisE"
                     /locus_tag="PLQ29_01200"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HPG85287.1"
                     /translation="MLIPSIDILGGRAVQLVGGERFELDCGDPLELAERFSVLGELAV
                     VDLDAAMGSGSNLELVAELASRWPCRVGGGVRDESTMRRLLDSGARSVMVGTRATPEF
                     LSAFPRERLIAALDFKRGVVMTEGWKTSTGASVADKAAVLAPYVGGIIVTAIEIEGSL
                     SGIDLNASRALVESIRAAGFDGVLTLAGGVSSAAEVATLDAMGVDAQVGMALYKGVLD
                     PADAFAACLSSDRPDGLWPTVVCDESGVALGLAYSDAESLKAAITERSGVYRSRRRGL
                     WRKGESSGDRQTLLRVDADCDRDALRFTVRQSGAGFCHRGTRTCFGAVGAAGFGGDDR
                     GFALVSRTTERRLAEVAAGEAPAGSYTARLFTEPGLLDAKIAEEAAELCEARGPGRSA
                     EEAADLVYFVSVKLASEGTSLADVERVLDRRALRVTRRPGNAKSPYREQGNEAWKTGI
                     H"
     gene            complement(1494..2993)
                     /gene="hisH"
                     /locus_tag="PLQ29_01205"
     CDS             complement(1494..2993)
                     /gene="hisH"
                     /locus_tag="PLQ29_01205"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPG85288.1"
                     /translation="MSAALGQDVIIVDTGRSNAASLAAALERVGARPRLSTDPAAVRV
                     ASFAVLPGVGAFGAVMDRLRAMGVVEALKERVELGLPTLGACLGMQLFFGSSEESPGA
                     VGIGALPGAITAFGPGVRSPQFGWNRLSPPEPSVGGLLAGAEALGGRAACAYFANSYK
                     LDEAPVGWASATAYYGAPFVAAMARGPVLLCQFHPELSGAWGQALLERWLVAGGAPAS
                     APTESSSITAEPSMAEPGTVPRRGTLPRIIPCLDVVGGRVVKGVRFEELRDSGDPAEL
                     AARYEASGADEIVLLDISAGLEGRETAAETVRAVRRRIGIPLCVGGGVRSLADAERVL
                     AAGADKVALNSRGYRDPGLLTAIAREFGRQCCVAAVDATRRGRSSGVALDGAPVVVLD
                     AGRTETATPVTDWIRRAVDAGAGELLLTSRDRDGTRSGYDLELLHSACAAAPGTPVIA
                     SGGAASPEQILEGLQAGADAALLAGALHDGSLTIAAIKDYMRREGAEVR"
     gene            complement(2990..3592)
                     /locus_tag="PLQ29_01210"
     CDS             complement(2990..3592)
                     /locus_tag="PLQ29_01210"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HPG85289.1"
                     /translation="MNDTRSPRAAVVERRTKETDVRVELTLAPGPVDVRTGVGFLDHL
                     LSSLAFHGGLSLSLRCDGDIGVDDHHSAEDCALALGSALKDALGDKVGIRRFGSAFAP
                     LDESLARAVVDLSGRPCAVVDLGLTREALGGLACENVAHVVASLANAAMATIHLDVLR
                     GSNDHHRAEAAFKALALALREAIALDGTYGAAVPSAKGAL"
     gene            complement(3589..4701)
                     /locus_tag="PLQ29_01215"
     CDS             complement(3589..4701)
                     /locus_tag="PLQ29_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPG85290.1"
                     /translation="MTTTETSVRPERPSSRSPWIPASAAYEPRRPDPRIRWFLDANEG
                     RPSPSVVAAIGEALSGATSGVGRYPSFYELESAVAAAWGVEPARVVATAGGDDAISRV
                     VAARLAPGAKMLVHEPAFEMFGAYAASRGGVTLALRWMDGESFPLEATVEAIERCPSL
                     GLVTVVSPSNPTGGIVPEADALTIAAACARSGAAMLFDAAYGEFAETDPSARLVAEGS
                     AYVVRSFSKAYGLAGLRIGYVIAPDAPSAASLRACGMPYPSSGLACAAAIAALADRSA
                     MLSAVATARAERDAMAARFRALGARVADSGANFVLARVRDPAALAAAMARAGVAVRSY
                     GGRAGLADAVRVSCPCDADGLAAVLGALDGAEEYIR"
     gene            complement(4698..5573)
                     /gene="hisG"
                     /locus_tag="PLQ29_01220"
     CDS             complement(4698..5573)
                     /gene="hisG"
                     /locus_tag="PLQ29_01220"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPG85291.1"
                     /translation="MERKDGKLRLALPKGRMQEGVLRLLAEAGLDMRAAERDYRPAAG
                     LDWLDVKVLKPQNIIEMLAAGSRDLGFAGADWVAELGMDLVEAHRVETGIDPVRLVAA
                     APAAMLESGRLPERPLVVASEYEGLARRWIAERGLEAEFVRSYGATEVFPPEDADCIV
                     DNTATGSTLRANGLAVVDEVMSSTTRLYANRRAMDDPSRRDRVEWLAMLCASVLAARG
                     RVMLEVNVDAERLEGLIAILPAMKEPTVSRLLGEAGYAVKVAAPRSSLPALLPEISAR
                     GGTGIVITQPSQIVP"
     gene            complement(5842..6309)
                     /locus_tag="PLQ29_01225"
     CDS             complement(5842..6309)
                     /locus_tag="PLQ29_01225"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HPG85292.1"
                     /translation="MDTNSLRPFFDAAGSVFAQMFGLEPEASAPRALDGAESHDWEVS
                     GLLGIAGSSQGVLALRFTMALVDRLLEASGVESRDEQDRLATASGLVAEMTNIVAGNA
                     IGEIAGQNLEISPPVVIKGKNHQITWPKIAPVVAVDFKTSLGAFELAVCYNGK"
     gene            6562..8532
                     /locus_tag="PLQ29_01230"
     CDS             6562..8532
                     /locus_tag="PLQ29_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPG85293.1"
                     /translation="MTKGAGLEDALRASEGQLGTVRVELGAPPSMADVAAAREHYRKT
                     GMPVAFKAYARPGADSPLEAYSLSVAGDGSPSMLTLLLDEAAAAGQARVSIGGRPALG
                     PSAVGPDRRLVVPITAADSPDAGSGGAVVVELEAGPPESSRTARVAIADASPGEPRVL
                     LATRRTGARSVIEALRPVRTVSYDELGGAGIYRYELIVLDGPDLSSLDGGVAEAIATY
                     VERGAGSLLVAVDSPEVGKPGDCPAIEGLLPVDLSPRALSRLPDVAMAVALDVSGSMY
                     GDKLSLAKAIGLELFANLKATDEAGIILFDEEARWLYPLASVGRLDAERSLAPLRAGG
                     GTRLFPAVAEAIGSLEASGMPEKRLVVVSDGITEPADFDALAARAFRSGIAISAMAVG
                     GEYDKALLTRLSAGSGGRFYRVRDASEVPSLIIEDRKSVSRTVFSESRVAVVDMAGRP
                     AGHIDGMARLGPRPDALAFYSSAAGDPLLATRRVGSRSTVVYASDLYGRYGREFLENP
                     GTLSLLDALLDGISGESAPALTVTETADGLALTLAADYLVEPWAAIADGSGRVVANAP
                     FERVAPGHFHAAPGRLPIGSYTALVEDRGRTIARVALVYNGGMEGRPSDSAAVAAEYR
                     PPFWALPRSGSPWLIAFFASSLVATVILRFRR"
     gene            8529..9440
                     /locus_tag="PLQ29_01235"
     CDS             8529..9440
                     /locus_tag="PLQ29_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85294.1"
                     /translation="MRAVTVRRVPACRFALAAAAAAALAAVPLVAGAQSVAASGSAAA
                     SISPASSLPYAFNEAVVSSRTGLSGRLTVYGLGSDGERYELYRAELGYADAATVTFYA
                     WPSLEAIVTEGLDGDGLPFEARARLERSGFGPSPGAETEPDLSTLEFPMRGRMPGALE
                     SDRRPWPLALLALWAALFSLAPLAVLGGRGRLGTARRSGRYATRRGPAVAETVAAVSA
                     TIAIAVLAFALGHARPDLRSAPDDSGATLLGVRSPLEPLPLSSFDGYARLRFQRPPLV
                     IAGDNGRPALAPAPFQAAWALPGRPRE"
     gene            9433..>9710
                     /locus_tag="PLQ29_01240"
     CDS             9433..>9710
                     /locus_tag="PLQ29_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85295.1"
                     /translation="MSRRLGVASAAMVVGPLLVAALALPGEADVAAAASIAGGLPLAW
                     AAAVSLAASLDATGLAIALAGALPFGAWALASYAPRPSWPDLVALASAA"
BASE COUNT         1364 a         3506 c         3400 g         1440 t
ORIGIN      
        1 cgagctcggc tgccaggcgg gcgagcgctg cctcgccgcc gtcccgtacc tcgtccatga
       61 tgcgcctagc cgtcgcctcg caggccgggt cgtcggccgg tcggccccta cggcctatct
      121 cctcgaggcc gacgcgcctc agtgtatacc ggtcttccac gcttcgttcc cctgctccct
      181 atacggcgac ttggcgttac cgggccgcct ggtcacgcgc agagcgcggc ggtcgagcac
      241 gcgctcgacg tccgcgaggc tcgtcccctc gctggccagc ttcaccgata cgaaatagac
      301 gaggtcggcc gcctcctcgg cggagcgccc cggtccccta gcctcgcata gctcggcggc
      361 ctcctcggct atcttggcgt cgagcaagcc cggttccgtg aagagtctgg ccgtatatga
      421 gcccgccggc gcttcgcccg cggcgacttc ggcgaggcgc cgctccgtcg tcctcgatac
      481 cagagcgaag ccgcgatcgt cgccgccgaa cccggccgcc ccgacggccc cgaagcaggt
      541 ccgcgttccc cggtggcaga acccggcgcc agactggcgc acggtaaaac gtagcgcgtc
      601 tcggtcgcag tcggcgtcga ctcgtaggag agtctggcgg tcgccgctcg actcgccctt
      661 gcgccacagc ccacgcctac gcgagcgata gacgccggag cgctccgtta tggcagcctt
      721 aaggctctcc gcgtccgagt acgcgagccc gagcgcgacg cctgactcgt cgcagacgac
      781 cgtgggccac agcccgtcgg gccggtcgga agacaggcac gcggcgaacg cgtccgcggg
      841 atcgagtacg cccttgtata gggccatgcc gacctgcgcg tcgacgccca tcgcgtcgag
      901 ggtagccacc tcggcggccg agctcactcc accggcgagc gtcagcaccc cgtcgaagcc
      961 ggccgcgcgt atcgactcga caagcgcgcg ggacgcgttc aggtcgatac ccgacagcga
     1021 gccctctatc tcgatagcgg tgacgatgat accgccgacg tacggagcca gcacggcggc
     1081 tttatcggct acgctcgctc cggtgctggt cttccagccc tcggtcatga cgacgccgcg
     1141 cttgaagtcg agggcggcta tgagacgttc cctggggaaa gcggacaaga attccggcgt
     1201 cgccctggta ccaaccatga cggagcgggc gcctgagtcg aggagccggc gcatcgtcga
     1261 ctcatcgcgc acgcccccgc cgactctgca cggccagcga gaagccagct ccgccacgag
     1321 ctcaaggttg gagccggagc ccatggcggc gtccaggtcg acgacggcca gctcgccgag
     1381 tacggagaag cgctccgcga gctcgagcgg atccccgcag tccagctcga aacgctcgcc
     1441 cccgacgagc tggacggccc tgccgcccag gatatcgatc gatggaatca gcatcagcgt
     1501 acctccgcgc cctcgcggcg catgtagtcc ttgatcgcgg caattgtcaa cgagccgtcg
     1561 tgcagggcgc cggcgagcag cgccgcgtcg gctccggcct gtaggccttc cagtatctgc
     1621 tccgggctgg ccgctccgcc cgacgctatc accggggttc cgggcgcggc ggcgcaggcc
     1681 gaatgcagga gctcgaggtc gtagcctgag cgggtgccgt ctcggtcacg gctcgtcaaa
     1741 agaagctcgc cggccccggc gtcgacggcc cggcgtatcc agtccgttac cggggtagcc
     1801 gtctctgtcc ggcccgcgtc cagcacgacg acgggcgcgc cgtcaagcgc gacgccggac
     1861 gaccggccgc gcctcgtcgc atcaaccgcg gcgacgcagc actggcggcc gaactcgcgc
     1921 gcgatcgccg tcaagagacc cgggtcgcga tagcccctgg agttgagggc gaccttgtcg
     1981 gcgccggcgg cgagcacgcg ctcagcgtcg gcgagactcc tgacgccgcc tccgacgcac
     2041 aggggtatgc ctatcctccg cctgacggcc cgcacggtct ccgccgcggt ttcgcgccct
     2101 tcgaggcccg cgcttatgtc gagcaggacg atctcgtcgg ctcccgacgc ctcgtagcgc
     2161 gccgccagct ccgctgggtc gcccgagtcg cgcagctcct cgaagcgtac gcccttgacg
     2221 acgcggcccc cgaccacgtc gaggcatgga atgatcctcg gcagggttcc ccgcctcggt
     2281 accgtgccgg gctccgccat actcggttcc gccgtgatcg atgacgactc ggtcggagcg
     2341 ctcgctggag cgcctcccgc gaccagccag cgctcgagca gggcctggcc ccaggccccg
     2401 gacaattcgg ggtggaactg gcagagcaac acggggccgc gcgccattgc cgcgacgaac
     2461 ggcgcgccgt agtacgcggt cgcggaggcc cagccgacgg gcgcctcgtc gagtttgtag
     2521 gagttggcga aataggcgca cgcggccctt ccgccgagcg cttctgcacc agccagcaga
     2581 ccgccgactg aaggctccgg tggcgacagg cggttccaac cgaactgcgg cgagcggacg
     2641 ccgggcccga acgcggtgat ggcgccggga agcgcgccaa tgcccacggc gccaggcgac
     2701 tcctcgctgg agccgaagaa cagctgcatg cccaggcagg cgccgagcgt cggtaggccg
     2761 agctcgactc gttccttgag cgcctcgact acgcccatgg cgcgcagccg gtccatgacg
     2821 gcgccgaagg ccccgacgcc gggtagcacc gcgaaagaag cgacccggac ggccgcggga
     2881 tcggtgctca gcctcggcct agcgcccacc cgctcgagag cggcggccag gctggccgcg
     2941 ttcgaccgtc ccgtgtcgac gatgatgacg tcttgtccta aggcggcgct catagggccc
     3001 ccttggcgct cggcacggcg gctccgtacg tgccgtcgag cgctatggcc tcgcgcaggg
     3061 ccaaggcgag cgccttgaag gccgcctcgg cccggtggtg gtcgttcgag ccgcgtagca
     3121 cgtccaggtg tatcgtcgcc atggcggcgt tcgcgaggct cgccacgaca tgggcgacgt
     3181 tctcgcaggc caggccgccg agggcctcgc gggtcaggcc gaggtcgacg acggcgcagg
     3241 gccggcccga caggtcgacg acggcgcgcg ccagcgattc gtcgagcggc gcgaaggccg
     3301 aaccgaatcg gcgtatgccg accttgtcgc ccagcgcgtc cttgagcgcc gagccgagcg
     3361 ccagcgcgca gtcctcggcg ctgtggtggt cgtcgacccc gatgtcgccg tcgcagcgta
     3421 aggacaggct gagtccgccg tggaacgcga ggctcgatag gaggtggtcg aggaagccga
     3481 cgccggtacg cacgtcgacc gggccgggcg cgagcgtaag ctcgacccgt acgtccgtct
     3541 ctttggtgcg ccgctcgacg accgcggcgc gaggacttcg ggtatcgttc atcggatata
     3601 ctcctcggcc ccgtccaggg ccccgagtac ggcggccagt ccgtcagcgt cgcaggggca
     3661 gctgacgcgg acggcgtcag cgagccccgc tcggccgccg tagctcctga cggcgacgcc
     3721 cgcgcgggcc atcgctgcgg cgagggcggc cggatcgcga acgcgcgcca gcacgaagtt
     3781 ggcgcctgaa tcggcgaccc gggcgccgag agcgcggaag cgggcggcca tggcgtctcg
     3841 ctcggcgcgc gcggtcgcga ccgcggatag catggcggac cggtcggcca gagccgcgat
     3901 ggcggccgcg caggccaggc cggagctcgg gtacggcatg ccgcaggcgc gaagcgaggc
     3961 ggcggacggg gcatccggcg ctatgacgta gccgatgcgc aggccggcta gcccgtaggc
     4021 cttggagaag ctgcgcacga cgtaggccga gccttcggct accaggcgag cgctcgggtc
     4081 ggtttcggcg aattccccgt aggccgcgtc gaacagcatg gccgcgccgc tcctggcgca
     4141 ggcggccgcg atagtcaacg cgtcagcctc ggggacgatc ccgcccgtgg ggttggacgg
     4201 ggagacgacg gtaaccagcc cgagcgacgg gcaacgctct atagcctcta cggtggcttc
     4261 cagcgggaag gattcgccgt ccatccaacg tagagcgagc gtaacgccgc ccctggaagc
     4321 ggcgtaggcg ccgaacatct cgaaggccgg ctcgtggacg agcatcttgg cgcctggcgc
     4381 gaggcgcgcc gcgacgacgc ggcttatggc gtcgtctccg ccggccgtag ctacgaccct
     4441 cgccggctcg acgccccagg cggccgccac cgcggattcc agctcgtaga acgacgggta
     4501 gcggccgact ccggacgtcg cccccgatag cgcctcgcct atcgcggcga cgaccgaggg
     4561 cgacggccga ccctcgttgg cgtcgaggaa ccaacggata cgcgggtctg gccgcctcgg
     4621 ctcataggcg gcgcttgccg gtatccacgg agagcgcgac gacggccgtt ctggccggac
     4681 ggaggtctct gtcgtcgtca tggcactatc tgcgacggct gggtgatgac gatgccagta
     4741 ccgccccgcg cgctgatctc aggcaggagg gcgggtagcg acgaccttgg cgcggccacc
     4801 ttgacggcgt agccggcctc gcctaagagg cgcgagacgg tcggctcctt catcgccggc
     4861 aggatcgcta tgagcccttc gaggcgctcg gcgtcgacgt tcacctcgag catgacgcgg
     4921 cctcgggcgg cgagtacgct ggcgcacagc atcgcgagcc actcgacgcg atcgcgcctg
     4981 gacggatcgt ccatcgcgcg cctgttggcg tagagccgcg tcgtcgagct catcacctcg
     5041 tcgacgacgg ccaagccgtt ggcgcgcagc gtcgagccgg tcgccgtatt gtcgacgatg
     5101 cagtccgcgt cctccggagg gaagacctcg gtggccccgt aggagcgcac gaactccgcc
     5161 tccaggcccc gctccgctat ccatcgccga gccaagccct cgtactcgct ggcgaccacg
     5221 agagggcgct cgggcagtcg gcccgattcg agcatggccg cgggggccgc ggcgacgagg
     5281 cgcaccgggt cgataccggt ctcgacccgg tgcgcctcga cgagatccat accgagctcg
     5341 gccacccagt cggccccggc gaaaccgagg tcgcggctgc cggcggcgag catctcgatg
     5401 atgttctggg gcttgagcac cttgacgtcg agccagtcga ggccggccgc cggcctgtag
     5461 tcccgctcgg cggccctcat gtccaggccc gcctccgcga ggaggcgcag cacgccctcc
     5521 tgcatccttc ccttgggcag cgccaggcgc agcttcccgt ccttccgttc catgtcgttc
     5581 gctccttccg agatagaagg ccgcgcgcgg ccgcgtccgt ctcgcggagg cccggaagcc
     5641 ccgaagaaac gaaaacgccg gcctcgggcg gccggcgtat cgttccgttc ggttccgata
     5701 ctggaaccta gggtatgacg atcatcgcga ccgcataggc gtggccgtga tgatggagat
     5761 ggcagaactg tacgtatcga gcgttactca tcggagatac gctacacgga acggaggtcc
     5821 cgcgtcaagc gcctatcgag gctacttgcc gttatagcat accgccagct cgaaggcgcc
     5881 gaggctcgtc ttgaagtcga cggcgacgac cggcgctatc tttggccagg ttatctgatg
     5941 attcttgccc ttgatgacca ccgggggcga tatctcgagg ttctgccccg cgatctcgcc
     6001 gatggcgttg ccggcaacga tgttcgtcat ctcggccacg aggccggaag ccgtggccag
     6061 cctgtcctgc tcgtcgcgcg actcgacgcc gctggcctcg agaagcctgt cgacgagggc
     6121 catggtgaac ctgagcgcca gcacgccctg cgacgagccg gctatgccga gcagcccgga
     6181 aacttcccag tcatgggatt ccgccccgtc gagggcccgc ggggccgagg cctccggctc
     6241 gaggccgaac atctgcgcga agaccgagcc ggccgcgtcg aagaacggcc gcaaactatt
     6301 cgtatccatc gaatcgcatc ctcgtgatcg gttttagcat cgcggtttcg gttgtccgcc
     6361 agccgatacc gtattagaat agtgcgaacc atggcgaacc acaagggcga aaaggtaggc
     6421 gacgacacgg gccctcaggc gggcgccgcg agacgcgtaa ggttcgggct cgtcatcgct
     6481 ctccccctcc tgacgctcgt cccctacgcg ctccggcttc ccatgcccct accgcgcctc
     6541 acgcttcctg tccgggacta cgtgacgaag ggggcaggcc tcgaggacgc gctccgcgcg
     6601 tccgaaggcc agctcggcac ggtccgcgtc gagctcggcg cccctccgtc catggccgac
     6661 gtcgccgcgg cgcgggaaca ctaccggaag accggcatgc cggtcgcgtt caaggcgtac
     6721 gcgcgccctg gcgccgattc gccgctcgag gcctattccc tgtccgtcgc gggggacggc
     6781 tcgccgtcca tgttgaccct gctgctcgac gaggcggccg ccgccggcca ggcccgcgtc
     6841 tcgatcggcg gacggcccgc gctcggcccg tcggcggtcg gccctgaccg caggctcgtc
     6901 gtgcccataa ccgccgctga ctcgcccgac gcgggatcgg gcggcgcggt cgtcgtcgag
     6961 ctggaggccg gtccgcccga atcctccagg acggctcgcg tcgcaatagc cgacgcttcg
     7021 cccggcgagc cccgcgtcct cctggccacg cggcggacgg gggccaggag cgtgatcgag
     7081 gcgctgcgcc cggtacgtac ggtgagctac gatgaactcg gcggcgccgg catctatcgg
     7141 tatgaactga tcgtgctcga cggcccggac ctatcgtcgc tcgacggagg cgtcgccgag
     7201 gcgatagcga cctatgtcga gcgcggcgcc ggcagcctct tggtcgcggt cgactcgccc
     7261 gaggtaggaa agcccggcga ctgtccggcg atcgagggtc tcctgccggt cgacctgtcg
     7321 ccgagggcgc tgtcgcgcct gcccgacgtc gcgatggctg tggccctcga tgtgtccggc
     7381 tcgatgtacg gggacaagct gtcgctggcc aaggccatag gcctcgagct gttcgccaac
     7441 ctcaaggcga cggacgaggc cggcatcatc ctgttcgacg aggaagcgcg ctggctgtac
     7501 ccgctggcgt cggtcggacg gctcgacgcg gagcgtagcc tggcgccgct ccgggccgga
     7561 ggcggcacga ggctgtttcc ggccgtcgcc gaggccatcg gctcgctcga ggcgtcgggc
     7621 atgcccgaga agcggctggt cgtcgtctcc gacggcatca ccgagccggc cgacttcgac
     7681 gcactggcgg ccagggcctt ccggtcgggc atcgcgatat cggcgatggc cgtcggcggc
     7741 gagtacgaca aggccctgct gacgcggctg tccgccgggt ccggcggccg gttctaccgc
     7801 gtcagggacg cgtccgaggt gccgtcgctg atcatcgagg accggaagag cgtctcgagg
     7861 acggtgttct ccgagagccg cgtcgccgtc gtcgacatgg ccgggcggcc cgcgggccat
     7921 atcgacggca tggccaggct cgggccgagg ccggacgcgc tggccttcta ctcgagcgcg
     7981 gccggcgacc cgctgctggc gacgaggcgc gtcggctcca ggtcgaccgt cgtctacgcg
     8041 agcgacctct acggccgcta cggccgcgag ttcctcgaga atcccggaac gctatcgcta
     8101 ctcgacgcgc tactcgacgg catatccggc gagtccgcgc cggcgctcac cgtgaccgag
     8161 accgccgatg gcctcgcgct gacgctggcc gccgactacc tcgtcgagcc atgggcggcc
     8221 atagcggacg gttcgggccg cgtcgtcgca aacgcgccgt tcgagcgcgt ggccccgggg
     8281 cacttccacg cggccccggg caggctgccg atcggctcgt acaccgccct cgtcgaggac
     8341 cgcggccgga ccatcgctcg ggtggccctg gtgtataacg gcggcatgga ggggcggccg
     8401 agcgattcgg cggcggtcgc cgccgagtac cgccccccgt tctgggccct gccgaggagc
     8461 ggttcgccct ggctgatcgc gttcttcgcc tcgtcgctcg tagcgaccgt gatcctgagg
     8521 ttcaggcgat gagggccgtg acggttcgcc gcgttccggc ctgccgcttc gctctggcgg
     8581 ccgctgcggc ggccgcgctg gccgccgtcc ccctcgtcgc cggggcgcag tcggtcgcgg
     8641 cgtcgggttc agccgccgcc tcgatatcgc ccgcctcgtc gctaccctac gccttcaacg
     8701 aagccgtggt ctcgagccgt acgggcctat cgggccgctt gacggtctac ggcctcggtt
     8761 cagatggaga acggtatgag ctctacaggg ccgagctcgg ctacgccgac gccgcgaccg
     8821 tcaccttcta tgcctggccc agcctcgagg ccatagtcac cgaaggtctg gacggcgatg
     8881 ggctgccctt cgaggctcgc gcgcggctcg aacgctccgg cttcgggccc agtcccgggg
     8941 cggagaccga gccggacctg tcgacgctcg agttcccgat gcgcgggcgc atgccgggtg
     9001 cgctcgagtc cgacaggcgg ccatggccgc tcgccctgct agcgctctgg gccgccctgt
     9061 tctcgctggc gccgctcgcc gttctcggcg ggcgaggccg acttggtacc gccaggcgct
     9121 cgggacggta cgcgacgagg cgcggccccg cggtcgccga gacggtcgcg gccgtctcgg
     9181 cgacgatcgc gatcgccgtg ctggccttcg cgctcggcca tgcccggccg gacctacgct
     9241 cggccccgga cgatagcggc gcgaccctgc tcggcgtccg ctcgccgctc gaacccctgc
     9301 cgctgtcgtc gttcgacggc tacgcgaggc tgaggttcca gcggccgccg ctcgtgatag
     9361 cgggcgacaa cggccggccg gcgctcgccc cggccccgtt ccaggcggcc tgggcccttc
     9421 cagggaggcc tcgtgagtag gcgtttgggc gtcgcgagcg cggcgatggt ggtgggtccg
     9481 ctcctggttg cggccttggc gctaccgggt gaggccgacg tggctgcggc cgcctcgata
     9541 gccgggggct tgcccctcgc ctgggccgcc gccgtgtcgc tcgcggccag cctggacgcg
     9601 accgggctgg ccatcgccct ggccggcgct ctgccgttcg gcgcctgggc cttggcctcc
     9661 tacgcgccta ggccgtcctg gcccgacctg gtcgcgctgg cctcggcggc
//
LOCUS       DAOPHV010000023         9656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_8761_length_9656_cov_4.455265, whole genome shotgun sequence.
ACCESSION   DAOPHV010000023 DAOPHV010000000
VERSION     DAOPHV010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9656
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8761_length_9656_cov_4.455265"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1939)
                     /locus_tag="PLQ29_01245"
     CDS             complement(<1..1939)
                     /locus_tag="PLQ29_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPG85296.1"
                     /translation="MDHGLLPRHARACVKTIAIVLTLIACQNVNLEMSEKPDRPANLS
                     ATPADAAVMLAWDEAARAEGYEVYWKAGQSVSRDDHAGRLDLTEAEATVSGLINGQSY
                     AFIVVAVNDSGAGEPSQAVTCAPTPPPAAPLDVAASPGDSLAVVSWASSQGADGYVLY
                     YRAGGAVAPGDQGVQRKVDVSSPYTVMGLENGVIHAFAVAAYNAAGEGSPSAIATTTP
                     SNANAPHDPSPADGADGVPLSGPLSLTWLTTNPNGNQLSFDVYLGLGSIPSSPIESGL
                     PTPACSVDGPFEPHAEYRWRVSVNNISQGYRSEGPEWSFMTANTPPPAPTLSDPAQGA
                     TDVDLSTWFHWNHAHDPDEDAVAYDLLIDTANPPATVAATGLTANGYQVNGLTADTLY
                     YWRVVARDGYGGSAESSVRSFRTIDPPAAPTALAASADPRYAKVNLSWTSPGGPVSGY
                     RIYRSMATGGQGSTPIATTTGWSFEDTGFFIGDCAGTYYYRVSAYNAAGEGPMSAESL
                     ATGSAPTIPVPSTELQPHNADYHFQDRYFLAITPNSDGALSSRQYRLNRIGPDPQLSW
                     VSGDVAIYVNPIFAEAAGPVTCTYRLQSRNEWGDSAWSGEFSVVVPAALPIIYPTITY
                     AGAGRLDFTWSNPAGATKLRIF"
     gene            complement(2034..3461)
                     /locus_tag="PLQ29_01250"
     CDS             complement(2034..3461)
                     /locus_tag="PLQ29_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPG85297.1"
                     /translation="MTQTMRSPGAGAGAGPAVAYAAFGYGWAMVFFNYPSAWSSLSTF
                     PAAETIPATGAFFVAACVSMFACSFIGDGLLGVAAVLAGLMASGSAIAMAAWPGTIPL
                     GAIAVAMGAAAGLTYAIGLLFLVHRAPAPFDPRIVGVCIASFSAVSGVLLALLRIRGA
                     AVALAVALPLCSAAAGLRVRRSVEREPIEERGLPVRPALGLVYLIALNYIAYNAATVT
                     SIYGSAGFIAGKPALDWAFRAFVAIAAVATIRRFNVVKMLYGSYIMIMAALAFSVMGP
                     RGRLPYGLLINTAFVLTEFGYASLLVRLGRGSKARPALFALGTLVVALPSLVGNHLGA
                     WLSPRLGEASLLTIGAGLALYCVALPFVPGLLRSIEGAFAATPAGGDDGQESFIRETF
                     FSRLPAERSLDAIESSYDDDYRLSPREREVAALLLDRYDYDAIAARLGISLNTVKSHA
                     RSIYRKYDIRGRKDLIALSTNATNG"
     gene            complement(3626..4627)
                     /locus_tag="PLQ29_01255"
     CDS             complement(3626..4627)
                     /locus_tag="PLQ29_01255"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPG85298.1"
                     /translation="MTNIESLVPDGFSGCVSVSRDGSIVFRKAFGYADKANERLNTVD
                     TRFATASAGKFFVAIAVMRLVEEGRLGLEDPIGSILRFDLRAIDPGVTIRQLLTHTSG
                     VPDYFDESVMDEYADLWVDFPCYRIRRSADLLPLFVDKPMMYPAGQRFQYNNSGFVLL
                     DLAIEAVTGAPFDRFLSQAVFDPAGMSSTGYYETDRLPSRCANAYIYDEEREEYYTNI
                     FSVDAKGTGAGGAFTTAPDVSSLWRALLSGGLLPTARVEELLTARIEDEDEFYGYGVW
                     LAKRNGALVPYFQGCDPGVSFISSLERSRGLEITIESNQCDDVWALERSIYEAMAGR"
     gene            4855..6039
                     /locus_tag="PLQ29_01260"
     CDS             4855..6039
                     /locus_tag="PLQ29_01260"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HPG85299.1"
                     /translation="MKGILDKTRALETSLAEMKELLLGNVVLLGEVPSYAGTWQSDDE
                     MSSVDYSVRARFFADRIGELGVDECTSDPLGNPIGIIKGGEPGRPPILVCAELDSLFN
                     PPGDIHYAIDGDTIIGPGLMDNAIGAAAVMSLPDVLKREGLAFKSTIMLAGIANTMRD
                     SKNLTLVERFLESLGTKPGAAIIVKGGELGRLNYFSEAVVRADILCERRPGARQSPPN
                     MIIVANEIIDRLLELELPQKPETAVTVGMIRGGYKYGTPATTARVGIELRSTSNEALA
                     ALSSRIAGILDQVRHERRVDIEYDVAAALGATSLGWSHPLTSATAAVIQELGLELNVY
                     PSVSELYYFLQRGIPAVTIGVATGSDYHQELASASLRSLYSGMAQLIGVMLAADEEAA
                     DA"
     gene            6032..7024
                     /locus_tag="PLQ29_01265"
     CDS             6032..7024
                     /locus_tag="PLQ29_01265"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HPG85300.1"
                     /translation="MRNGQDWLSSRTFHHSEFSDIQALVERKRERGLSISLCFPTLNE
                     EATIAKEIVIVKSELQMRYPLVDEIVVIDSGSTDRTREVAAEFGAKVYLATDILPCIE
                     PYKGKGENLWKALYVLEGDIIVYVDADIKNIHPRFVYGLVGPLLKNDDTRFVKAFYDR
                     PIALDQKTVKPSGGGRVTELVTRPLFSSFYPELAQFIQPLSGEYAGFRECFDTIPFPI
                     GYGVETSMLMDIYDRFGLEAMAQVDLDKRVHRNQSTVALGRMSFGIIQTFIAKLRASG
                     KATMAGELYKTMIQFELEGGAYRRKESAIDIVERRPIVELPEYLAKFPARAGKA"
     gene            7021..8427
                     /locus_tag="PLQ29_01270"
     CDS             7021..8427
                     /locus_tag="PLQ29_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG85301.1"
                     /translation="MRVLVVHYHLKPGGVTTVIRGQLAALASRGVEAALLVGEAPPDE
                     RWNGAPLAVDPSLAYDEPGSGGLPDAAKVAALASTIQREAWRLASGGGDVVVHVHNPT
                     IRKNASLLPALSALARSGVRLALHVHDLAEDWRPDVYSGQPYPEGATWAAINRRDAEA
                     LSAAGAGSVRFLPNLLPRATAGEPPREPPAGSGLVLYPVRGIRRKNLGEALLLSLFMR
                     PGGSVGVTLPPNSPRDRPYYDGWKAAALELGAPVAFELGLARGLDELYGEARAVVTTS
                     VKEGFGLSYLEPAIRGRATLGRRLPWVVDDFEAEGLAFPALYGSIATPRGLFDGEAFG
                     RRAAEVVREAARAYGLASEALPEAVVGRLMDGDTVDYGRLDELAQLEVLRSVARSPEA
                     RSAIISANPFIDGWDQAAEVAARIAPSALEPWSEAAYADRLDGLYAAALSGGGAAPDK
                     AALLELYLRPEAFHGVGV"
     gene            8411..9418
                     /locus_tag="PLQ29_01275"
     CDS             8411..9418
                     /locus_tag="PLQ29_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPG85302.1"
                     /translation="MASASSLSEALIAELRRSIADNGIGRPQSPDGYERFLSPARLPS
                     ELSRLVGKAPRERPLAIAVDVYGTLLASAIGEIGPSAEWGETDDAAEEAAREAFPSDM
                     AERLRAIVEEDHARSRARGVPWPEVDAPSAFARALGLGLEDGARTCVAWECTINRCAP
                     MPGAAEFLAVCAKKGVPLGIVSNAQFYTRLFIEEAFESDLYRGEADEADEADEADEAD
                     EADDARPEGASGRPCLGFDPELALWSYETGRAKPDRWMFDELARRLSARGIPADRILY
                     IGNDALNDCAAAGEAGLMTALFAGDARSCRPRSGDDRVAAHPPSLVVYSWDDLRRLLC
                     T"
     gene            9409..>9656
                     /locus_tag="PLQ29_01280"
     CDS             9409..>9656
                     /locus_tag="PLQ29_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPG85303.1"
                     /translation="MYLTKTREITRVGFVSTRFQGTDGVSLETWKWNDVLTELGYETF
                     FFSGRSDWFPERSMVVEDAFFGQPRVKELHDEIFDRSTR"
BASE COUNT         1622 a         3187 c         3274 g         1573 t
ORIGIN      
        1 ggaaaatcct gagcttcgtc gctccggcgg gattggacca cgtgaagtcg agccttcccg
       61 ccccggcgta ggtgatcgtc gggtagataa tggggagcgc ggccggcacg acgaccgaga
      121 actcgcccga ccacgccgaa tcgccccatt cgttgcggct ctgcaggcga taggtgcatg
      181 taacgggtcc ggcagcctcg gcgaagatcg ggttgacgta gatggcgacg tcgcccgaaa
      241 cccaggaaag ctgcggatcg ggaccgattc ggttcaggcg gtactgcctg gatgatagcg
      301 ctccatccga attcggagtg atggccagga aatagcggtc ctggaagtga tagtcggcgt
      361 tatgaggctg caactcggtc gacggaaccg gtattgtggg cgccgagccg gtcgcgaggc
      421 tttcagcgct catcggcccc tcgccagcgg cgttataggc gcttacccgg tagtagtacg
      481 taccggcgca gtccccgatg aagaagccgg tatcctcgaa gctccaaccc gttgtcgtgg
      541 ctatcggggt cgagccctgg ccgcctgtag ccatcgatct gtagatccta tacccgctca
      601 cggggcctcc ggggctcgtc caggataggt ttaccttggc gtaccgagga tctgcgctcg
      661 ccgcgagcgc ggtcggcgcc gccggcgggt cgatcgtcct gaaactgcgg accgaactct
      721 cggccgatcc gccgtagccg tccctggcca cgacgcgcca gtagtacagc gtgtcagccg
      781 ttaatccgtt cacctggtac ccgttggcgg taaggcccgt cgcggctacc gtggccggtg
      841 ggttcgcggt atcgatgagc aggtcgtagg cgacggcgtc ctcgtccggg tcatgggcgt
      901 ggttccagtg gaaccaggtc gataggtcta cgtcggtcgc gccctgggcc ggatcggata
      961 gcgtcggggc gggcggcggg gtattcgccg tcatgaagct ccattcgggc ccctcgctac
     1021 ggtagccctg gcttatgttg ttcaccgaga cgcgccagcg atactcggcg tgcggttcga
     1081 acggcccgtc gacgctacag gcgggcgtgg gcagcccgga ttcgataggc gaggatggaa
     1141 tcgagccgag gcctaggtac acgtcgaagg aaagttgatt gccgttcgga ttggtcgtca
     1201 accaggtgag cgacaacggc cccgaaaggg gaacgccgtc ggctccgtcg gccggtgacg
     1261 ggtcgtgcgg cgcgttagcg ttcgacggcg tcgtcgtggc aatcgcgcta ggagagccct
     1321 ctccggcggc gttataggcc gccacggcga acgcgtggat aacgccgttc tcgaggccca
     1381 tgaccgtata cggcgagctg acgtcgacct tgcgctggac accctggtcg cccggcgcga
     1441 cggcgccgcc ggccctgtaa tagagcacgt agccgtcggc cccctggctc gaggcccagg
     1501 aaacgacggc cagcgagtcg cctggcgagg ccgcgacgtc gagcggcgcg gccgggggcg
     1561 gcgtcggagc gcaggtgacg gcctggctgg gctccccggc ccccgagtcg ttgaccgcga
     1621 cgactatgaa ggcgtaggac tgcccgttga tcaggccgct caccgtcgcc tcggcttccg
     1681 tcaggtccag gcgcccggcg tggtcgtcgc ggctgaccga ttggcctgcc ttccagtaga
     1741 cttcgtagcc ttcggccctc gccgcctcgt cccacgcgag cataacggcc gcgtcggccg
     1801 gcgtggcgct caggttggcg gggcggtccg gtttctcgct catctcgagg ttgacgttct
     1861 ggcacgctat gagcgtaagg acgatcgcga tcgtctttac acaggctcga gcgtggcgcg
     1921 gcaataagcc gtgatccatg ggaacctcct gacggccggc gctcggcgcc gtccatcgtc
     1981 cataggtatc ccggtacccg gccgctttca atcacacgaa aagtgtgagc ccatcacccg
     2041 ttcgtggcgt tggtcgagag ggcgatgagg tccttccttc ctcggatgtc gtatttccgg
     2101 tagatggacc tggcgtggct cttgacggta ttcagggata tcccgagcct cgccgcgatg
     2161 gcgtcgtaat cgtaacggtc gagcagcagt gcggcaacct cccgctccct cgggctcagc
     2221 cggtaatcgt catcatagct cgactcgatg gcgtcgagcg accgttccgc ggggagcctg
     2281 gagaagaacg tctcgcggat gaacgattcc tggccgtcgt cgcccccggc gggcgtcgcc
     2341 gcgaaggccc cctctatgga gcgcaggagc ccgggcacga agggcagcgc gacgcagtac
     2401 agcgccagtc cggcgccgat ggtcagcaag gacgcctcgc cgaggcgtgg cgatagccag
     2461 gcgcccaggt ggttgccgac gaggctcggc agcgcgacga cgagcgtgcc gagggcgaac
     2521 agcgctggcc gagcctttga tccgcgccca agccggacga gtagcgacgc atagccgaat
     2581 tccgtaagca cgaaggccgt attgatcagg agcccgtacg ggaggcggcc tctcggaccc
     2641 attacggaaa aggccagggc cgccatgatc atgatatagc tgccgtacag catcttgacg
     2701 acgttgaagc gacgtatcgt agctaccgcc gcgatggcga cgaaggccct gaacgcccag
     2761 tctaaggcgg gcttgccggc tatgaacccc gcgctcccgt agatcgaggt taccgtcgcg
     2821 gcgttatagg cgatatagtt cagcgcgatg aggtagacca agcccagggc cggccgtaca
     2881 ggcaggccgc gttcctcgat cggctcgcgc tcgacggagc gccggacgcg cagcccggcc
     2941 gccgcgctac agagcggtag cgcgacggcg agcgcgacgg ccgcgccacg tatccgtagt
     3001 agcgccagta ggacgccgga taccgcgctg aaggaggcga tgcacactcc gacgatcctc
     3061 gggtcgaacg gcgccggcgc ccggtgcacg agaaacaata agccaatggc gtaggtaagc
     3121 ccggcggcgg cgcccatggc gacggctatg gcgccgagcg ggatcgtccc gggccatgcg
     3181 gccatggcga tagcggagcc ggacgccatg agtcccgcta gaaccgcggc caccccgagc
     3241 agtccgtcgc cgatgaacga acaggcgaac atcgatacgc aggcggccac gaagaacgcg
     3301 cccgtcgccg ggatcgtctc cgcggccggg aacgtcgaga ggctggacca ggcgctcgga
     3361 taattgaaga acaccatggc ccacccgtat ccgaaagccg cgtacgctac cgccggcccc
     3421 gcgccggctc cagcgcccgg cgaacgcatc gtctgggtca tacctacgtc gctccgtcgc
     3481 gacgttcgcc ccggggctgg ctcgacgaag gcccgggggc gaccgatcgc ggatcaggat
     3541 ctgttagctc gtatggtata ccatggcttt cggtggctgg taacgaaaaa aaacaccacc
     3601 gttcgccgct actctggccc tcccgctatc gcccggccat cgcctcgtat atgctccgtt
     3661 caagcgccca gacgtcgtcg cactggttgc tctcgatggt gatctcgagg ccgcgggacc
     3721 gctcgaggct cgagatgaag ctgacgccgg gatcgcagcc ctggaagtag ggaacgagcg
     3781 cgccgttccg tttcgcgagc cagacgccgt agccgtagaa ctcgtcctcg tcctcgatgc
     3841 gcgccgtcag gagctcctcg acgcgagccg tgggcaggag cccgcccgac agcaacgccc
     3901 gccagagcga cgatacgtcc ggggcggtcg tgaaggcccc tccggctccg gtacccttcg
     3961 cgtcgacgct gaagatgttc gtgtagtact cctctcgctc ttcgtcgtag atatacgcgt
     4021 tggcgcagcg cgaggggagg cggtcggtct cgtagtagcc ggtcgacgac atgccggccg
     4081 ggtcgaagac cgcctggctc aggaacctgt cgaacggggc gcccgtgacg gcctcgatcg
     4141 ctaggtcgag cagcacgaag cccgagttgt tgtactggaa ccgctggccg gccggataca
     4201 tcatcggctt gtcgacgaac agcggtagca ggtcggccga gcgcctgata cggtagcagg
     4261 ggaaatcgac ccatagatcc gcgtactcgt ccatcacgct ctcgtcgaag tagtccggca
     4321 cgcccgaggt atgcgtcagg agctgcctga tcgtcacgcc agggtcgata gcgcgcaagt
     4381 cgaagcgcag tatcgagccg atgggatctt cgagccccag ccgcccttcc tcgacgagcc
     4441 gcatgacggc gatggcgacg aagaacttgc cggccgacgc ggtggcgaac ctcgtgtcga
     4501 ccgtgttcag ccgctcgttg gccttgtcgg cgtagccgaa ggccttcctg aacacgatgg
     4561 aaccgtctcg cgatacggag acgcaaccgc tgaagccgtc cggtacgagg ctttcgatgt
     4621 tcgtcataca cgctttcctt attccttgag cttgatcgct tgcgatcatt aaagctcgat
     4681 cggagggccg aggcaagcga ctcatcgtgt aaaccaggaa agtagacggc tcggggctcc
     4741 atcgcagacg gacgtttgcc agctcgggcg ctatcccgta tactggatcg tggccggccc
     4801 gggaatcagc cgtaggccac ggtcgcgagg aggtacagac tggatccagc cgtcttgaag
     4861 ggtatcttgg acaagacgcg ggcgctcgaa acgtcgctcg cggagatgaa ggagctgttg
     4921 ctcgggaacg tggtgctgct cggggaggtg ccgtcgtacg cgggcacctg gcagagcgac
     4981 gacgagatgt cgagcgtcga ctatagcgtg cgcgcgcggt tcttcgccga tcgcatcggc
     5041 gagctcggcg tcgacgagtg caccagcgat ccgctcggca atcccattgg catcatcaag
     5101 ggcggcgagc ccgggcgtcc gcccatactc gtctgcgccg agctcgactc cctgttcaac
     5161 cctcccggcg acatccacta cgccatcgac ggcgatacca tcatcgggcc cgggctcatg
     5221 gacaacgcta tcggcgccgc tgcggtcatg tcgcttccgg acgtgctcaa gcgggagggc
     5281 ctcgcgttca agtcgacgat catgctggcc ggcatcgcca atacgatgcg cgatagcaag
     5341 aacctgacgc tcgtcgaacg cttcctggag agcctcggca cgaagccggg cgcggccatc
     5401 atcgtcaagg gcggcgagct gggtcggctc aactatttct cggaagccgt ggtccgcgcc
     5461 gatatcctct gcgagcgccg gccgggagcg cgccagtcgc cgccgaacat gatcatcgtc
     5521 gccaacgaga tcatcgaccg gctcctcgag ctggagctgc cgcagaagcc ggagacggcc
     5581 gtcacggtgg gcatgatacg cgggggctac aagtacggga ccccggcgac gacggcccgc
     5641 gtcggtatag agctacggag cacctcgaac gaggcgctcg ccgccctgtc gagccgcatc
     5701 gccggcatcc tcgaccaggt ccgccacgag aggcgcgtcg acatcgagta cgacgtggcg
     5761 gccgcgctcg gggcgacgag cctcggctgg tcccatccgc tgacctcggc aacggcggcg
     5821 gtgatccagg agctcggcct ggagctcaac gtctacccga gcgtgtcgga gctgtactac
     5881 ttcctgcagc gcggcatacc ggcggtcacg atcggcgtcg cgaccggcag cgactaccac
     5941 caggagctgg cgtccgccag cctgcgctcc ctgtactccg gcatggccca gctgatcggc
     6001 gtcatgctcg cggcggacga ggaggcggcc gatgcgtaac ggccaggact ggctgtcgag
     6061 ccgcaccttc caccatagcg agttctccga catacaggcc ctcgtggagc gcaagcgcga
     6121 acgcgggctg tccatatcgc tgtgcttccc gacgctcaac gaggaggcga cgatcgccaa
     6181 ggagatcgtc atcgtcaaga gcgagctgca gatgcgctat cccctcgtcg acgagatcgt
     6241 cgtgatcgac tccgggtcca cggaccggac gcgggaggtg gctgccgagt tcggcgccaa
     6301 agtctacctc gccacggata tcctgccgtg catagagccg tacaaaggca agggcgagaa
     6361 cctctggaag gcgttgtacg tcctcgaggg agacatcatc gtctacgtgg acgccgacat
     6421 caagaacatc catccgcgct tcgtgtacgg gctcgtcggc cccttgctca agaacgacga
     6481 cacgcgcttc gtgaaggcgt tctatgaccg ccccatagcc ctcgaccaga agaccgtcaa
     6541 gccttcgggc ggcgggcgag tcaccgagct ggtaacgagg ccgctgttct ccagtttcta
     6601 tcccgagctg gcccagttca tccagccgct gtcgggcgag tacgccggct tccgcgaatg
     6661 cttcgacacg ataccgttcc ctataggcta cggcgtcgag acgagcatgc tgatggacat
     6721 ctacgaccgc ttcggcctcg aggccatggc gcaggtcgac ctggacaagc gggtgcatcg
     6781 caaccagtcg acggtggccc tcggccgcat gtccttcggc atcatccaga ccttcatagc
     6841 caagctcagg gcgtcgggca aggcgacgat ggccggggag ctctacaaga cgatgatcca
     6901 gttcgagctc gagggcggcg cctatcggcg caaggagagc gccatcgaca tcgtcgagcg
     6961 ccggcccatc gtcgagctac ccgagtacct ggccaaattc cccgcgaggg ccggcaaggc
     7021 atgagggtcc tcgtcgtcca ttaccacctg aagcccgggg gcgtgaccac ggtcatacgg
     7081 gggcagctcg cggccctcgc ctcgcggggc gtcgaggcgg cgctcctcgt gggcgaggcg
     7141 cctccggacg agcgctggaa cggggcgccg ttggccgtcg atccgtcgct ggcctacgac
     7201 gagccgggat cgggcgggct tccggacgcc gccaaggtag cggcgctagc ctcgacgatc
     7261 cagcgagaag cctggaggct ggcgtccggc ggcggtgacg tcgtggtcca cgtccataat
     7321 ccgacgatac gtaagaacgc ctccctcctg ccggcgctat cggcgctggc tcgctcgggc
     7381 gtccgcctgg ccctccatgt ccacgacctg gccgaggatt ggcggcccga cgtgtactcc
     7441 ggccaaccct atcccgaagg cgcgacctgg gctgccatca accggcgcga cgccgaggcc
     7501 ctatcggccg ccggggcggg ttcggtacgc ttcctgccca atctcttgcc ccgggccacg
     7561 gccggggaac cgccgcgcga gcccccggcc ggatcaggcc tggtcctgta ccccgtccgg
     7621 ggcatacggc gcaagaacct cggcgaggcc ctgctcctat cgctgttcat gcggccgggc
     7681 ggcagcgtcg gcgtgaccct gccgccgaac agccctcgcg acaggccgta ctacgacggc
     7741 tggaaggccg ccgcgctcga actcggggca ccggtcgcct tcgagcttgg cctcgcgcgt
     7801 gggctcgacg agctgtacgg cgaggcgagg gccgtcgtga cgacctcggt aaaggaaggc
     7861 ttcggcctgt cctacctgga accggccatc cggggcaggg cgaccctggg gcgacgcctg
     7921 ccatgggtcg tcgacgattt cgaggccgag ggcctggcgt tcccggccct gtacggctcc
     7981 atcgctactc cgcgcggcct gttcgatggc gaggctttcg gccgtcgagc cgccgaggtc
     8041 gtgcgcgagg ctgcccgggc ctacggcctc gcctctgagg cgttgccgga agccgtggtc
     8101 ggccgcctga tggacggaga caccgtcgat tacgggcggc tcgatgagct ggcccagctc
     8161 gaggtcctgc gctccgtcgc ccgctcgccc gaagcccgct ccgctatcat ctcggccaat
     8221 ccgttcatcg atggctggga ccaggcggcc gaggttgccg cccgaatcgc gccaagcgcc
     8281 ctcgaaccgt ggtcggaggc cgcctacgcc gaccggctcg atgggctgta tgcagcggcc
     8341 ttgtctggcg gcggcgcggc gcccgacaag gccgcgctgc tcgagctgta cctcaggccg
     8401 gaggcctttc atggcgtcgg cgtctagcct gagcgaggcg ctgatcgccg agcttaggcg
     8461 gtcgatagcc gataacggga taggacggcc gcaatctccc gacggctacg aacgcttcct
     8521 atccccggct cgcctaccgt ccgaactctc ccgtctcgtc ggcaaggcgc cgcgagagcg
     8581 cccgctggcg atagccgtcg acgtctacgg cacgctgctc gccagcgcca taggcgagat
     8641 cgggccgagc gccgaatggg gcgagaccga cgacgccgct gaggaagccg cccgtgaggc
     8701 cttccctagc gacatggccg agcgtctgcg cgccatcgtc gaggaagacc acgctcgctc
     8761 gcgcgcccgc ggcgtaccct ggcccgaggt ggacgccccc tcggcgttcg ctcgcgcgct
     8821 cggactgggg ctcgaggatg gggcgaggac ctgcgtcgcc tgggaatgca cgataaaccg
     8881 ctgcgcgccc atgcccggtg ccgccgagtt cttagccgtc tgcgccaaga agggggtgcc
     8941 cctcggcatc gtatcgaacg cccagttcta cacgcgcctg ttcatagagg aggcctttga
     9001 gtccgatcta taccgcggcg aggcggacga ggcggacgag gcggacgagg cggacgaggc
     9061 ggacgaggcg gacgacgcgc ggcccgaagg cgcctccggc cggccttgcc tcggtttcga
     9121 tcccgagctg gcgctctggt cctacgagac gggccgcgcc aaacccgatc gttggatgtt
     9181 cgacgaactc gcccgacgcc tgtccgcccg cggcatcccg gcggacagga tcctgtatat
     9241 aggcaacgac gcgctcaacg actgcgccgc cgcgggggag gcgggcctga tgacggccct
     9301 gttcgcgggc gacgctcgat cctgcagacc gcggtccggg gatgaccggg tggcggcgca
     9361 tcccccgtcg ttggtcgtgt attcctggga cgacctgagg aggctgctat gtacctgacc
     9421 aagacgcgcg agataacccg cgtcggcttc gtgtcgaccc gctttcaggg tacggacggc
     9481 gtatcgctcg agacttggaa atggaacgac gtgctgaccg agctgggcta cgagacgttc
     9541 ttcttctcgg gcagaagcga ctggttcccg gagcgctcga tggtcgtgga ggacgccttc
     9601 ttcgggcagc cccgcgtcaa ggagctgcat gacgagatct tcgaccggtc gacgcg
//
LOCUS       DAOPHV010000024         9546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_8887_length_9546_cov_4.330945, whole genome shotgun sequence.
ACCESSION   DAOPHV010000024 DAOPHV010000000
VERSION     DAOPHV010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8887_length_9546_cov_4.330945"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..2876
                     /gene="clpB"
                     /locus_tag="PLQ29_01285"
     CDS             267..2876
                     /gene="clpB"
                     /locus_tag="PLQ29_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPG85304.1"
                     /translation="MDYDRFTLKAQEAIQAAHASAQKADHSQIELEHLLIALLDSDGS
                     AVRPLLQRLGASVDELESRLEASLAKKPKVYGHASQVYLSPAAGKALAKAEAEAAALK
                     DDYVAGEHVLLAMLDDDGSVGPLLKEFGVTKTGALEALKSVRGNRSVKDQAAEEKYRV
                     LEKYCRDLTALAHREKLDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LARRIVSGDVPDSLKRKKLLALDLGSLVAGAKFRGEFEERLKAVIEEVQKSDGAVILF
                     IDELHTLVGAGAAEGAMDASNLLKPALARGELKCIGATTLDEYRKHIEKDAALERRFQ
                     PVLCPEPSVEDTIAILRGLKERYEVHHGVRIMDEALIAAATLSDRYITSRFLPDKAID
                     LVDEAASRIKMEIESQPTDLDQIERRILQLTIEKQALSRESDPASLERLAKLDAELTE
                     LTARRDAMRLRWKNEKDRIEVVRRLKEDIERLKAEEQRHERDGNLAKAAEIKYGSLLE
                     AQKRLDEAAGAAKDAAGADGSRLLREEVSEEDIARVVSTWTGIPVSKMMSSEIQKYLE
                     LEKILEKRVVGQEKAIVAVSDAIRRNRAGLSDPNRPLGSFLFVGPTGVGKTELAKTLA
                     EFLFNDEKSLTRIDMSEYMEKHTVSRLIGAPPGYVGYDEGGQLTEAVRRRPYSVVLFD
                     EVEKAHPDVFNALLQLLDDGRLTDGQGRTVDFKNCIVIMTSNLGSELILEAKDMDELR
                     GRVAELLKTAFKPEFLNRIDETVIFERLKPEDMGGIVDIQLAALAKRLADRSLSVSMT
                     DAAKALVARIGYDPRYGARPLKRVMQAEIQNPLSRLVLKGELREGDAVVIDAVKGGAV
                     QDGSFAFKVRRRA"
     gene            2869..3669
                     /locus_tag="PLQ29_01290"
     CDS             2869..3669
                     /locus_tag="PLQ29_01290"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPG85305.1"
                     /translation="MLEVEATGARLGLTDTHAHLSYIAERLGRRAIDELAAAYADAGA
                     MVLDPGVDYDDYPVRKAAFGDLGFVRLAAGIWPDAESIGDAEARVAALEAHLRDPACV
                     AVGECGLDYHWMHGGPSEQAELFSAQAELAVRLGKPLIVHSRDAHRDTLAIVRPVASR
                     IPVIIHCFGYDEEAAREYAAAGCYVSFAGNLSYKKSGALRAACAAVPGERLLLETDSP
                     YMCPEPRRGKDATPLDIGRTYALAAALRGSSIDDLAELVARNAAALFG"
     gene            complement(3735..4472)
                     /locus_tag="PLQ29_01295"
     CDS             complement(3735..4472)
                     /locus_tag="PLQ29_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HPG85306.1"
                     /translation="MKKIIAIVLAFSALASVSAQGTVGEPDASSIGIDTAQQKLQVVT
                     VDKFESAGFWNVYIMPDEGVANGRLFTGGPAAKADEPKPEERYVGIDPKIADVQTYGA
                     RVDFFKRGFNTVYVLAQKPIPIEGITKTISVWVVGRNYNHTLKIIVSDFFNNQFELTM
                     GKLNFHGWKKMSVAIPPQNPDGRTGIIQRNYHYNSQMGLKVVGFKIECDPMEAFGTYY
                     VYFDDLRAVTDLFAEDSRDEDDMVDGW"
     gene            complement(4482..5270)
                     /locus_tag="PLQ29_01300"
     CDS             complement(4482..5270)
                     /locus_tag="PLQ29_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HPG85307.1"
                     /translation="MKQRVVKAICLSFVIVFMLAATGAFADDETVNLESLVVQTFDEP
                     DSQPWFLIGSKFATKGYPKLAYAKSWPVALFGTTGGDKDLRSLGVAMLFDRKEYNWVD
                     VVPGSKSGEGEEATYKPIELPLPGRVKMLDMWIWSGNFDYYIEAYIRDYTGIVHVLPM
                     GDLGHVGWKNFRVNIPNNVPQSKKYLPKSENLTLVKFRIWTRPTEVVASPTKADAPIE
                     QKAVYFYFDQLKVLTDTFESLFDGDSLMDPKVIEETWGSSDSSK"
     gene            complement(5388..6278)
                     /locus_tag="PLQ29_01305"
     CDS             complement(5388..6278)
                     /locus_tag="PLQ29_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85308.1"
                     /translation="MRQVPGSVERRRSARSLRLLATLSAALACSCGGLAGSLAVVDPV
                     LAYLSPAAAEAFGSYAEALVVLPDEGASAALYAAIDTRAPATLFLSPLLASEIPYILA
                     RDGSTRVACVAITAPTPDPRLCAAVFSPADAASVAGAMAAEEAIRLARNASPPVVAAV
                     LSSGDEAREAFVAAYAAAGGPGEPVVELAEGGFSKAIAERLRTMDVRVAYVSVPPDQT
                     ARWLESAFDGYAFRIVAEAFPADGLRPSSDATIAWDLDATLADLAGLLRSGGSGNVPG
                     RWKAETSRPRRANKPVEVGR"
     gene            6392..7390
                     /locus_tag="PLQ29_01310"
     CDS             6392..7390
                     /locus_tag="PLQ29_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HPG85309.1"
                     /translation="MTSYKELGLCNTRELFKKAVAGGDAIPAYNNNNMEQMQAIIQAC
                     VETRSPVILQVSSGARKYANSNLLRNMARGAVEYAAELGYKIPIVLHLDHGDSYELCV
                     DCIESGFSSVMIDGSHLPYDENVALTRKVCEYAHSRTDYVTVEGELGVLAGVEDEVSA
                     EHSSYTQPDEVQDFVSKTGVDSLAISIGTSHGRTKFKPSQCTRSADGVLIPPPLRFDI
                     LAEIEKRLPGFPIVLHGSSSVPMEYVRIIEQFGGKLSDSVGIPEEQLRKAAKSAVCKI
                     NIDSDGRLAMTAMIRKVFAEKPDEFDPRKYLGPARDELKKLYMHKNKEVLGSAGQA"
     gene            7502..8617
                     /locus_tag="PLQ29_01315"
     CDS             7502..8617
                     /locus_tag="PLQ29_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPG85310.1"
                     /translation="MSKRILACLAMATAMVASAAADDELAFGVEGVRVFSGFIPSGGD
                     VSLTYTGFQLYEPGVTKLYLEAGGGYEDRSIVRDLMTGDPYVADASGYEYRSPNFQWE
                     LAFIQGLARRDDGKNLVEAFAYYRGRYDDYLTEADGLSDAVFADIGGLFGTSVMGGLS
                     YDSGSTSGHKQKTGLYAEATAEWGPGFLNPLTDFWRVSAQARYFVPLYDMESDGGNLL
                     NLYAGFFAGVDYSDGESVPIYVNQSFGGRNLRGSVGDCVRGYGWNKFDSSLKAASSAE
                     LRLVGPAIVIADIVPVLFGFVDAGYYRGYSESTTRFDVNGLLASAGGGLAIDVFGFAQ
                     LSFIAGARLVDDELGYQYSGFLGAADAFYGIKFFLHF"
     gene            complement(8794..9255)
                     /gene="rnhA"
                     /locus_tag="PLQ29_01320"
     CDS             complement(8794..9255)
                     /gene="rnhA"
                     /locus_tag="PLQ29_01320"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HPG85311.1"
                     /translation="MNAPYIVYSDGACSGNPGPGGWAWVSLAGGEIREGYGGEPATTN
                     NRMELIGATEALAAIKARVDAGELKPGLVEVRTDSQYVKNGVTSWIASWKRNGWRTAS
                     KQPVKNRDLWERLDALSRELAARWVWVEGHAGEAWNERCDELARRGVAENR"
     gene            complement(9252..>9546)
                     /locus_tag="PLQ29_01325"
     CDS             complement(9252..>9546)
                     /locus_tag="PLQ29_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85312.1"
                     /translation="RLHEEYRKDTLVRQAGELNRVMRLARAALPRVRSRLLVVSGELD
                     ASVPASAAARIASTSRLSPEIRLETLKGVGHLFPFLPDGRHDCARLVEEWFEE"
BASE COUNT         1729 a         3099 c         3075 g         1643 t
ORIGIN      
        1 gcttccagcc gaggttcgtc tccgtgctgg tcgcgaaggc taggccgtcc tgcgcaccga
       61 gcgccgcctt gacggcccgc atcgccgcta tcgcctcgtc cctggtcagg ccgtgcatca
      121 cgaccacctt gccgtccggt atagtcatac cgtcaatcta accaccatgt cgccgattgg
      181 gcaagggcgg gtcggccgga tacgcccgcg cttgccgacc ggagccgtgg cttggtatgt
      241 tctagttcag cgaatggaga acacccatgg attacgatag gttcacgctc aaggcccagg
      301 aggccatcca ggcggcccac gcctccgccc agaaagcgga tcattcccag atagagcttg
      361 agcatctgct gatcgctctt ctcgactccg acggctcggc cgtcaggcca ctgctccagc
      421 gcctcggcgc ctccgtcgac gagcttgagt cgaggctcga ggcctcgctg gccaagaagc
      481 ccaaggtcta cggccatgcc tcccaggtct acttgtcacc ggctgctggc aaggccctgg
      541 ccaaggcgga ggcggaggcc gcggccctca aggacgacta cgtggcgggc gagcacgtcc
      601 tgctcgcgat gctcgacgac gacggttcgg tcggcccgct ccttaaggaa ttcggcgtga
      661 ccaagaccgg cgccctcgag gccctcaagt cggtccgcgg caaccgcagc gtcaaggacc
      721 aggccgccga ggaaaagtac cgcgtcctcg agaagtactg ccgcgatctg accgccttgg
      781 cccaccgcga gaagctcgac ccggtgatag gccgcgacga ggagatacgc cgcgtgatgc
      841 aggtcctctc gaggcgcacc aagaacaacc cggtgctcat aggcgagccg ggcgtcggca
      901 agacggccat cgtcgagggc ctggcccgcc gcatcgtctc cggcgatgtg cccgattcgc
      961 tcaagcgcaa gaagctcctc gccctcgacc tgggttcgct cgtcgccggc gccaagttcc
     1021 gcggcgagtt cgaggaacgg ctcaaggccg tcatcgagga ggtgcagaag tcggacggcg
     1081 ccgtcatcct gttcatcgac gagctccata ccctggtcgg cgccggcgcg gcggagggcg
     1141 cgatggacgc gtcgaacctc ctcaaacccg ccctggcccg cggcgagctc aagtgcatcg
     1201 gcgccaccac cctggacgag taccgcaagc acatcgagaa ggacgcggcc ctcgagcggc
     1261 gcttccagcc cgtgctctgc ccggagccgt cggtcgagga caccatagcc atactgcgcg
     1321 gtctcaagga gcgctacgag gtgcaccacg gcgtccgcat catggacgag gcgctcatcg
     1381 ccgcagccac gctgtccgac cgctacatca ccagccgctt cctgccggat aaggccatcg
     1441 acctggtcga cgaggcggcg agccgcatca agatggagat cgagagccag ccaaccgacc
     1501 tcgaccagat cgagcggcgc atactccagc tgaccatcga gaagcaggcc ctgtcgcgcg
     1561 agtccgaccc ggccagcctc gagcgcctgg ccaagctcga cgccgagctg accgagctga
     1621 cggccaggcg cgacgcgatg cgcctgcgct ggaagaacga gaaggaccgc atcgaggtcg
     1681 tccgcaggct caaggaggac atcgagcggc tcaaggccga ggagcagcgc cacgaacgcg
     1741 acgggaacct ggccaaggcg gccgagatca agtacggtag cctgctcgag gctcagaaac
     1801 gcctcgacga ggcggccgga gccgcgaagg acgcggccgg ggccgacggc tcgcgcctcc
     1861 tccgcgagga ggtctccgag gaggacatag cccgcgtcgt gtcgacttgg accggcatac
     1921 ccgtgtccaa gatgatgtcg agcgagatcc agaagtacct cgagctcgag aagatactcg
     1981 agaaacgggt cgtcggccag gaaaaggcca tcgtcgccgt gtccgacgcc atacgccgca
     2041 accgcgccgg cctgtccgac ccgaaccggc cgctcggcag cttcctcttc gtcggcccca
     2101 cgggcgtcgg caagaccgag ctggccaaga ccctggctga attcctcttc aacgacgaga
     2161 agtcgctcac gcgcatcgac atgagcgagt acatggagaa gcacacggtc agccgcctca
     2221 taggcgcgcc gcccgggtac gtaggctacg acgagggagg ccagctgacc gaggcggtca
     2281 ggcgacggcc gtactcggtg gtgttgttcg atgaggtgga aaaggcccat ccggacgtct
     2341 tcaacgccct cctgcagctc ctcgacgatg gccgcctgac cgacggccag ggccgcaccg
     2401 tcgacttcaa gaactgcatc gtcatcatga cgagcaacct cggctcagag ctgatcctcg
     2461 aggccaagga catggacgag ctacgcggcc gcgtggccga gctccttaag acggcgttca
     2521 agccggagtt cctcaaccgc atcgacgaga cggtcatctt cgagcggctc aagcccgagg
     2581 atatgggcgg catcgtcgac atccagctgg cggcgctggc caaacgcctg gccgaccggt
     2641 ccctgtcagt atcgatgacc gacgcggcga aggctctggt cgcgaggatt ggctacgatc
     2701 cgcgctacgg cgcgcgcccg ctcaagcgcg tgatgcaggc ggagatccag aacccgctgt
     2761 ctcggctcgt gctcaagggc gagctgcgcg agggggatgc ggtggtcatc gacgccgtta
     2821 agggcggcgc cgtccaggac ggctcgttcg cgttcaaggt gcgccgccgt gcttgaagtg
     2881 gaggctacgg gcgcgcggct cggcctgacc gatacgcacg cccatctgag ctacatcgcc
     2941 gagcggctcg gtcggcgggc tatcgacgaa ctggccgccg cgtacgctga cgcgggcgcc
     3001 atggtactag accctggcgt cgactacgac gactatccgg tcaggaaggc cgctttcggc
     3061 gacttgggct tcgtgcgcct ggccgcgggc atctggccgg acgccgagtc aatcggcgac
     3121 gccgaggccc gggtcgccgc gctcgaggcg cacctgcgcg acccagcctg cgtcgcggta
     3181 ggggagtgcg ggcttgacta ccactggatg cacggcggac cgtccgagca ggccgagctg
     3241 ttctccgctc aggccgagtt ggccgtacgc cttggcaagc cgctgatcgt ccactcgagg
     3301 gacgcgcacc gcgatacctt ggcgatcgtg cggcccgtgg cctcgcggat accggttatc
     3361 atccactgct tcggctacga cgaggaagcg gcgcgggagt acgcggcggc cggctgctac
     3421 gtatccttcg cgggtaacct gagctacaag aagtcgggcg cgctcagggc ggcctgcgcc
     3481 gccgtgccgg gcgagcgcct cctcctcgag accgattccc cgtacatgtg ccccgaacca
     3541 cgccgcggca aggacgcgac tccgctcgac ataggcagaa cctacgccct ggccgccgcg
     3601 ctacgcggca gcagcatcga cgatctggct gagctcgtgg ctcggaacgc cgcggctctc
     3661 ttcggctgag gcgcgagacc ataaaaaaag aagaccccgt cgccggggtc ttcttttttt
     3721 atgggaagcg actattacca gccgtcgacc atgtcgtcct catcgcggga gtcctcggcg
     3781 aagaggtcgg tgacggccct caggtcgtcg aagtagacgt agtaggtgcc gaaggcttcc
     3841 atcgggtcgc actcgatctt gaagcccact accttgaggc ccatctggct gttgtagtgg
     3901 taatttcgct ggatgatgcc ggtcctgccg tcggggttct gcggcgggat ggccacggac
     3961 atcttcttcc agccgtggaa gttgagcttg cccatagtca gctcgaactg attgttgaag
     4021 aagtccgata cgatgatctt gagcgtgtgg ttgtagtttc tgcccacgac ccagacggag
     4081 atggtcttgg tgatgccctc gatcgggatg ggcttctggg cgagtacgta gacggtgttg
     4141 aagccgcgct tgaagaagtc gaccctggcg ccgtaggtct gcacgtcggc gatcttgggg
     4201 tcgatgccga cgtagcgctc ctcgggcttg ggctcgtcgg ccttggctgc cgggccgccc
     4261 gtgaagagcc tgccgttggc gacgccctcg tcgggcatga tgtacacgtt ccagaagcca
     4321 gcggactcga acttgtccac cgtgaccacc tggagtttct gctgggccgt atcgatgcct
     4381 atggagctgg cgtcgggctc gccgacggtc ccctgggcag agacggaagc gagggcggaa
     4441 aaagccaata cgatggcgat tatctttttc atggtctccc cttacttaga cgaatcggaa
     4501 ctgccccagg tctcctcgat gaccttggga tccatgaggc tgtcgccatc gaacagcgac
     4561 tcgaaggtat ccgtgaggac cttgagctgg tcgaagtaga agtagacggc cttctgctcg
     4621 ataggggcat cggccttggt cggcgacgcc accacctcgg tcggcctcgt ccagatcctg
     4681 aacttgacga gggtgaggtt ttcgctcttg gggaggtact tcttggactg cggcacgttg
     4741 tttgggatgt tgacgcggaa gttcttccaa ccgacgtggc cgagatcgcc catcggcagc
     4801 acgtgcacga tgcccgtgta gtcgcggatg tacgcctcga tatagtagtc gaagttgccg
     4861 gaccagatcc acatgtcgag catcttgact cggccgggca acggcaactc gataggctta
     4921 taggtcgcct cttctccctc gccggacttg gagccgggga cgacgtcgac ccagttgtat
     4981 tccttgcggt cgaacagcat cgcgacgccg aggctgcgga ggtctttgtc gcctccggta
     5041 gtgccgaaca gggctaccgg ccaggacttg gcgtaggcca gcttgggata gcccttggtg
     5101 gcgaacttgc tgccgatcag gaaccagggc tggctatccg gctcgtcgaa ggtctgtacg
     5161 accagcgatt ccaggtttac ggtttcatcg tcggcgaagg cccccgtcgc cgcgagcatg
     5221 aatacgatga cgaaggacag gcagatcgcc tttacgacgc gctgtttcat caggactact
     5281 ccttaagttt tgcaccatct taacgataaa ccatctgcgg gtggaattat atttttaagc
     5341 catacctttg tcaaacaaat acggtcgccc gcgcacggta tatacgatta tcggccgact
     5401 tcgaccggct tattagctcg ccgaggcctc gaagtctcgg ctttccagcg cccgggcacg
     5461 ttgccggagc ccccggaccg tagcaagccg gccagatcgg ccaacgtcgc gtcgagatcc
     5521 cacgcgatcg tcgcgtcgga cgagggcctt aggccatcgg cggggaaggc ttcggcgacg
     5581 atacggaacg cgtatccgtc gaaagccgat tcgagccacc tggcggtctg atccggtggg
     5641 accgagacgt aggccactcg aacgtccatg gtccgtagcc tctcggctat cgccttcgag
     5701 aagcctcctt ccgccagctc gacgaccggc tctccaggac cgccggccgc ggcgtaggcg
     5761 gcgacgaacg cctcgcgggc ctcgtcgccc gacgacagca cggccgctac gacgggcggc
     5821 gacgcgttcc gagccaggcg gatcgcctcc tcggccgcca tggctccggc gaccgaggcc
     5881 gcgtcggcgg gcgagaatac cgccgcgcag agccgcgggt ccggagtcgg cgccgttatc
     5941 gccacgcagg cgacgcgggt cgagccgtct ctggcgagta tgtacggtat ctctgaagcc
     6001 aggagcgggg ataggaatag cgttgcgggt gcgcgggtgt ctatcgcggc gtagagcgcg
     6061 gccgaggcgc cctcatcggg tagtacgacg agggcctcgg cgtacgagcc gaaggcctcg
     6121 gccgcggcgg gcgacagata ggccagaacg ggatcgacca cggccagcga gccggcgagc
     6181 cctccgcacg agcaggcgag agcggccgac agggtcgcca atagccgtag agatctggcg
     6241 cttcgccgcc gttcaaccga gcccggaacc tgacgcatgc tcgcgatagt atccgagccg
     6301 aggcggcggg acaaggccgg aactgcccta ggccttgacg atactcgacc gggaacctag
     6361 actcgtcctg tactctcatg aaggagcgtt tatgacaagc tacaaagagc tagggctctg
     6421 caatactcgc gagcttttca agaaggccgt cgccggcggg gacgccatcc cggcctataa
     6481 taataataac atggagcaga tgcaggcgat catccaggcc tgcgtcgaga cgaggagccc
     6541 cgtgatcctg caggtctcct cgggcgcccg caagtacgcc aactccaacc tgctgcgcaa
     6601 tatggcgcga ggcgccgtcg agtacgcggc cgagctcggc tacaagatcc cgatcgtcct
     6661 ccacctcgac cacggcgaca gctacgagct atgcgtcgac tgtatcgaga gcggcttctc
     6721 gagcgtgatg atcgacggct cgcacctgcc ctacgacgag aacgtcgccc tgaccaggaa
     6781 ggtctgcgaa tatgcccatt cgaggaccga ttacgtgacc gtcgagggcg agctcggcgt
     6841 tctggccggc gtcgaggacg aggtgtcggc cgagcattcg agctataccc agcccgatga
     6901 ggttcaggac ttcgtctcca agaccggcgt cgactccctg gccatttcga tcggcacaag
     6961 ccatgggcgc acgaagttta agccgtcgca gtgcaccagg agcgccgacg gcgtgctgat
     7021 cccgccgccc ctgcgcttcg acatactcgc cgagatcgag aagcgcctgc ctggtttccc
     7081 gatcgtgctc catggctcgt cgagcgtgcc gatggagtac gtcaggatca tcgagcagtt
     7141 cggcggcaag ctgtccgact cggtcggcat ccccgaggag cagctgcgca aggccgccaa
     7201 gagcgccgtc tgcaagatca acatcgactc cgacggccgc ctcgccatga ccgcgatgat
     7261 acgcaaggtc ttcgccgaga agccggacga gttcgacccg cgcaagtacc tcgggccggc
     7321 ccgcgacgaa ctcaagaagc tgtacatgca caagaataag gaagtgctcg gatcggccgg
     7381 acaggcctaa acgaggaatc gccatagtcg aggggcccgc catgggtccc tctttttttt
     7441 gccgaatcgc gccgtagctg gtattatggc gcaatatcga tagcgttttt ggaggatcac
     7501 gatgagtaaa cgcatactag cgtgcctggc catggcgaca gccatggtcg catcagccgc
     7561 ggcggacgat gagctggcct tcggcgtcga gggcgtgcgc gtcttcagcg gcttcatacc
     7621 gtcgggaggc gacgtatcgc tgacctatac cggttttcag ctctacgagc cgggcgtaac
     7681 gaagctgtac ctcgaggccg gcggcggcta cgaggacagg tccatcgtcc gcgatctgat
     7741 gactggcgac ccctacgtcg ccgacgcctc cgggtacgag taccgatcgc ctaatttcca
     7801 gtgggagctg gccttcatcc aaggcctggc caggcgcgac gatggcaaga acctcgtcga
     7861 ggccttcgcg tactaccgcg gccgctacga cgactacctg accgaggccg acggcctgtc
     7921 cgatgcggtg ttcgccgaca taggcggcct gttcggcaca tcggtcatgg gcggcctgtc
     7981 ctacgactcc ggttcgacga gcggccacaa gcagaaaacc gggctgtacg ccgaggcgac
     8041 cgccgagtgg ggccccggct tcctgaaccc gctgacggat ttctggcgcg tatccgccca
     8101 ggcgcgctac ttcgtccccc tgtacgacat ggaaagcgac ggcggcaacc tgctcaacct
     8161 gtatgccggc ttcttcgccg gagtcgacta ctctgacggc gagtcggtgc ccatctacgt
     8221 gaaccagagc ttcggcggac gtaacctacg cggctcggtc ggcgactgcg tacgcggcta
     8281 cggctggaac aagttcgact cgtcgctcaa ggcggcctcg agcgccgagc tcaggctcgt
     8341 cggcccggcc atcgtgatag cggacatcgt gccggtgctg ttcggcttcg tcgatgctgg
     8401 ctactaccgc ggctacagcg agtcgacgac gaggttcgac gtgaacgggc tgctcgcctc
     8461 ggccggcggc ggcctggcca tcgacgtctt cggcttcgcc cagctcagct tcatcgccgg
     8521 cgctcgcctg gtcgacgacg aactcggcta ccagtactcg ggcttcctcg gcgccgccga
     8581 cgcgttctac ggcatcaaat tcttcctgca cttttaggaa cttcagacta gagaagataa
     8641 ccacggagac acggaggcac ggaggcacgg aggcacggag aatacagaga tagggagaaa
     8701 aagaaaggaa aagagattaa ctcttattac ttctctttct ctcttaaatt actccgtgtc
     8761 tccgtgtctc cgtggtatgc ctttgtaaaa aagctatcgg ttctcggcta cgccgcggcg
     8821 ggccagctcg tcgcaacgtt cgttccaggc ctcgccagcg tggccctcga cccagaccca
     8881 gcgggcggcg agctccctcg acagcgcgtc gagccgttcc cacaggtcgc ggttcttgac
     8941 cggctgcttg ctcgccgtgc gccagccgtt gcgcttccat gacgctatcc agctggtcac
     9001 cccgttcttg acgtactggc tgtcggtgcg tacctcgacg aggccgggct tgagttcgcc
     9061 ggcatcgacg cgcgccttga tggcggccag cgcctcggtc gccccgatca actccatccg
     9121 gttgttcgtc gtggcgggct ccccgccgta tccttcacgg atctcgccac cggcgagcga
     9181 tacccaggcc cagcctcccg gcccggggtt gcccgagcag gctccgtcgc tatagacgat
     9241 gtacggtgcg ttcattcctc aaaccactcc tcgaccaatc gggcgcaatc atgccgtcca
     9301 tcgggcagga atgggaagag gtggccgacg cctttcagcg tctcgagccg gatctcgggg
     9361 gagagccgcg acgtcgacgc tatgcgggcc gccgccgagg ccgggacact cgcgtcgagc
     9421 tcgcccgata cgacgaggag gcgtgagcgt acgcggggca gggcggctcg ggctagccgc
     9481 attacgcggt tcagctcgcc ggcctgcctg accagggtgt ccttacggta ctcctcgtgg
     9541 agcctg
//
LOCUS       DAOPHV010000025         9399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_9103_length_9399_cov_4.602419, whole genome shotgun sequence.
ACCESSION   DAOPHV010000025 DAOPHV010000000
VERSION     DAOPHV010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9103_length_9399_cov_4.602419"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2482
                     /locus_tag="PLQ29_01330"
     CDS             <1..2482
                     /locus_tag="PLQ29_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPG85313.1"
                     /translation="IALRTLETDLTLRDWYKSRYDAIMVDEFQDDNELQKRVLYCLAE
                     RRDRTDPGRGPAVGPGDLEPGVLFFVGDEKQSIYKFRGADVTVFRGLAAELASAPGGS
                     GNHGLDVNWRSEPGLIDFFNATFARVMPAPDDASAQPFEARFSSLGSGPATPGVEPTV
                     AYLESYEPEDGDYLPSSEAEAWRIAELARSIVDDGQIVAAKGPDGRKVARPCRWEDIA
                     ILFRSTASQNVVERYLRLLGVPYTAASTAGLYAESILGDLYAMLRLAVYPDDRLACAA
                     VLRGPFARLSDDSVFSLLSDGEGCALDRDGDGLPPDDRARLAAARETWRGLRSMADRE
                     PLRALVEYLWYGRGLRWNALKDLQTTAFLEHFDYAWSLAAAADARGDRLVDLVSSLEA
                     TIGRLDRFDENVSKESARGVSLMTVHLSKGLEFPVVILPDLDATGRNGLRSPVEPSGR
                     FGSSMRLLGRDDDPADPIAELCSALRKHERGEGDEAMDEDLAETTRLFYVACTRAVSR
                     LYLVGKVPRSADAAGRSFRGLFIRAWPWAGPASLGDEEFLSDRPGDAPPYLGVEYVPP
                     RTKLDYVGLVGSEAADQARGGGSGASRMAASAAAAVRAVPRRKGRWAVTQAAERIAEK
                     SPRVQSAAPPVDAAAEAAESAEGSLRETEFGTLCHQFVEALLARPGEPPELSGPVAVS
                     LARLPKGRADLVRAEALALAEGFVRSERGRAAIAARSAKASEGTVFEMEYPFVWRAAA
                     GQAGAFPIALTGSIDLVYGDRTGLWVVDFKTDRVVAPSRHAFQLAVYRDAAEAMFGRP
                     ARAVVHYLRHGEEREIVESPDLSLLEGE"
     gene            2721..3281
                     /locus_tag="PLQ29_01335"
     CDS             2721..3281
                     /locus_tag="PLQ29_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPG85314.1"
                     /translation="MNDTSPPVKRPGFFSIFGFFFGISAITIGGGYVMVPVIQRALEK
                     RGWLPEDEFYDLFATAQSVPGPLALNTAMFAGRKVAGLPGFLAAFLGILLPPFAAILL
                     LAALLGAIGDWPVVQGFLDGAFAVVPGLTAALAFNMIRKRRWTPLRAVMTVASAVAIT
                     LAGTWAVPTFFAVVAVSAFAERKGAC"
     gene            3275..3790
                     /locus_tag="PLQ29_01340"
     CDS             3275..3790
                     /locus_tag="PLQ29_01340"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPG85315.1"
                     /translation="MLRLALVFLKIGVAAYGGGWTIVGIIRTEIVGRAWMSAEEFAKL
                     VAMAQITPGPVALNAATLVGYRLHGLLGAAVSTLAVVAAPVALSLLIGGFFAKATKSG
                     ARLTEALKTGTIGLIAMTVWAFAPAAVSSGLNVAIALTAFAVSAFTRINPLWIILGSG
                     VIGAATRAFLG"
     gene            3868..5916
                     /locus_tag="PLQ29_01345"
     CDS             3868..5916
                     /locus_tag="PLQ29_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPG85316.1"
                     /translation="MNNERIMIVEDEKIIAIDLQRRLERFGYDVVGMAGEGTEAVAMA
                     LELTPDIILMDIMLAGDMDGVEAAKAVKASKDIPIIFLTAYTDERTLERAKEVEPYGY
                     ILKPFKERELYTTIDIALYKHEMASKLTRQERLFSAILQSINDGIVALDTDLNVSFMN
                     QVAEDIVGTSEREAKGKHVGTILSILDPATKSDMLTGMINVGKRSTFFTDVSLRNLAG
                     QTVVLDGAITKIHEQANSTDGYLITMRDITELRRLSDSLNYQSSHDSLTGLSNREEFS
                     YKLSEILKNVKMVGGSHALLVLDIDRFKAVNDTCGTLAGDELLRQIASHVQTNIQRND
                     LSARIGADEFAIILMNCELEDTINVGNRLQGAVQRQRFQWQATTFPITLSIGIVPIDR
                     DAGDIHAVLAAADDSCHISKEEGGNKITIFSAQDSKFKRRRGDMEWIGKINRAVEENR
                     FLLYYQPIEPLGAGAEPKIEILLRLVNEDGSISGPTDFIPAAERYNLMPQVDRWVLEN
                     SMRAFRLLSDQGSPLADRIFSINLSGPALLDDALIDTILYYLNKYSLKASDFCFEITE
                     TAAIQNLSYATRFMNRLKVEGFTFALDDFGAGFSSFGYLRTLPVDYIKIDGSFVQNID
                     ESPVSYTMVESINSIGHVMGLKTIAEFVKGPAIKDKLVELGVDYGQGYHFSKPLPLFD
                     "
     gene            complement(5918..6994)
                     /locus_tag="PLQ29_01350"
     CDS             complement(5918..6994)
                     /locus_tag="PLQ29_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85317.1"
                     /translation="MGGRMSFDELTTTGLSRAFSARRGLPEAAASTLGVRDSDGLALE
                     LAGRTCCLTMLAGSGSRWVASLEAARAAGRDMGADPRAPRGLYPVRNAMPFGPDPAPI
                     ASYALAAVRDLGHHVIVVRGWEREIEERILGPFGFAPGSYRFATQGAPGGKPRGHGDA
                     AFQTLGLWSGYDYVTVNFGGDASSPLTALASLAAMAALERELGDRAPGLLLPAALVDS
                     PAYPIGLGPGGLPASFGHAKLRGGAAKAGGAVESGAAGYTNVGLRVYRAEALREAIES
                     IRERYWSPDGGYAIPGNAPASDDLGGEFALDNVDALLAAEGRARLLPIAKPEELSPVK
                     SLDDLPRFERDVGSVCRDWSGPGR"
     gene            complement(6998..7894)
                     /locus_tag="PLQ29_01355"
     CDS             complement(6998..7894)
                     /locus_tag="PLQ29_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HPG85318.1"
                     /translation="MPIPEYPEFAPVTVDMRDDLYPALNLLADGVSEFTFSGLYLFRD
                     TYKYTASHISGSTYVISGEKRGERFFFTPCCIPPNATLERLFEDHCYLKNMSESQCKA
                     ERIRLEAAGYEVHEDRDNFDYLYDRKDLAELSGKAYHKKRNLVNAFLNSYSYEQKELD
                     ASNVADAIRVLDAWRVEKGIDGDYAAAREALELYERLGMRGAVYYVDGKPAGYALGEP
                     IAKARMFAVHFEKATGDYKGIYQFINQAFAQALPPYFRQVNREQDLGDEGLRQAKMTY
                     RPSGFVRKYRVMPTKPSFLPEA"
     gene            complement(7990..8226)
                     /locus_tag="PLQ29_01360"
     CDS             complement(7990..8226)
                     /locus_tag="PLQ29_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85319.1"
                     /translation="MPYEGVLAIVSIFIVAPCIVFGFILLAKRGRNRVDLARCAVREK
                     ELELERERLRLEALQLENRKLDLMIEHRIDSLKP"
     gene            complement(8230..>9399)
                     /locus_tag="PLQ29_01365"
     CDS             complement(8230..>9399)
                     /locus_tag="PLQ29_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPG85320.1"
                     /translation="YVVRSEDPAGNKTEKAIQRMVVDTRQTQAFVTASAAGFSPNGDG
                     MFDEMSFGLVVKLTDGIDSWKLAMVGDDGVTRRSFTGKGATIPAKLAWDGKSDDGQLR
                     QGAYTAVFTVDYLKGDRAQASSATFSLDTEGPKVAMTTSPRYFSPDNDGVDDELRISL
                     AVSDASVIDSWRFDIVEVAVSESAGAKRAERVFFSWSGRGKPAERLAWDGRSQKGELV
                     EAATDYPYRLTIVDELGNQTVSEGLISVDVLVIRDGDRLKIKVPSIVFRPDFADFKDL
                     PQETLDRNAEVLKRIAQILNRFKDYKIRVEGHANSIAKMTGAGQPAIDREETRELLPL
                     SENRAALVMQKLIEYGVDPKRLSVRGLGSSEPVVPFTDAENRWKNRRVEFILIKE"
BASE COUNT         1573 a         3181 c         3088 g         1557 t
ORIGIN      
        1 catagcgcta cgtaccctcg agaccgacct gacgctacgc gactggtaca agtcgcgcta
       61 cgacgcgatc atggtcgacg agttccagga cgacaacgag ctccagaagc gggtgctgta
      121 ctgcctggcc gagcgccgcg accgtaccga ccccgggcgt ggcccggccg tcggccccgg
      181 agatcttgag ccgggcgtgc tgttcttcgt cggtgacgag aagcagagca tctacaaatt
      241 ccgcggcgcc gacgtgacgg tgttccgcgg cttggccgca gagctggcct cggcgcccgg
      301 cggctcggga aaccacggcc tggacgtcaa ctggcgctcc gagcccggcc tgatcgactt
      361 cttcaacgcc accttcgcgc gggtcatgcc cgcccccgat gacgcgtccg cccagccctt
      421 cgaggccagg ttctcatcgc tcggctccgg cccggcaacg ccaggcgtcg agccgacggt
      481 ggcctacctc gaatcgtacg agcccgagga tggggattac ctgccttcga gcgaggccga
      541 ggcctggcgc atcgccgagc tggcgcgctc catcgtcgac gatggccaga tcgtggcggc
      601 caagggcccc gatggccgca aagtcgcccg gccctgccgc tgggaggaca tagccatcct
      661 gttccgctcg acggccagcc agaacgtcgt cgagcgatac ctgcgcttgc tcggcgtccc
      721 ctatacggcc gcgtcgaccg cgggcctcta cgccgagtcg attcttggcg acctgtacgc
      781 gatgctcagg ctggcggtct acccggacga ccgcctggcc tgcgccgccg tgctccgcgg
      841 ccccttcgcg aggctctcgg acgattcggt cttctcgctg ttgtccgacg gcgaaggctg
      901 cgcgctggac cgcgacggcg acggcttacc gccggacgat cgcgccaggc tggccgccgc
      961 ccgagagacc tggcggggcc tgcgctccat ggccgaccgc gagccgctgc gcgccctcgt
     1021 cgagtaccta tggtacggac ggggcttgcg ctggaatgcg ctcaaggatc tccagacgac
     1081 ggccttcctc gagcatttcg actacgcctg gagcctggcc gccgcggccg acgcgcgcgg
     1141 cgatcggctg gtcgacctcg tatcgtcgct cgaagcgacg ataggccggc ttgaccgctt
     1201 cgacgagaac gtttcgaagg aaagcgctcg aggcgtatcg ctgatgaccg tccacctgag
     1261 caaggggctc gaattccccg tcgttatact gcctgacctc gacgcgaccg gtcggaacgg
     1321 cctccgctcg ccggtggagc cctcgggccg cttcgggtcg tcgatgcgcc tcctcgggcg
     1381 ggacgacgat ccggcggatc cgatagccga gctatgctcg gctctgcgca agcacgagcg
     1441 cggcgaaggc gacgaggcta tggacgagga cctggccgag acgacgaggc tgttctacgt
     1501 cgcctgtacc agggccgttt caaggctcta tctggtcggg aaggtaccgc ggagcgccga
     1561 cgccgcgggc cggagcttcc gcgggctgtt catacgcgcc tggccctggg ccggcccggc
     1621 tagcctgggc gacgaggagt tcctatcgga tcgcccgggc gatgctccgc cctatctcgg
     1681 ggtcgagtac gtaccgccgc ggaccaagct cgactacgtc gggctcgtcg gtagcgaggc
     1741 cgcggaccaa gcgcgaggcg gtgggtccgg ggcgtcgcgc atggcggctt cggccgccgc
     1801 cgccgtccgg gccgtgccga ggcgaaaggg ccgctgggcg gtcacccagg cggccgagcg
     1861 gatagcggaa aagtcgccga gggtccagag cgcggcaccg cctgtcgacg ccgccgccga
     1921 ggccgccgag tccgccgagg gctcgctacg ggagacggag ttcggcacgc tctgccacca
     1981 gttcgtcgag gcgctcctag ctcggccggg agaaccgccc gagctttccg gcccggtggc
     2041 cgtctcgctc gcccgcctgc ccaagggtcg cgccgacctc gtacgcgcag aggcgctcgc
     2101 gctggccgaa ggcttcgtgc gatcggagcg ggggcgggcc gcgatcgccg cgcggtcggc
     2161 caaagcctcg gaaggaaccg tcttcgagat ggagtacccc ttcgtctggc gggccgccgc
     2221 aggtcaggcc ggggccttcc ccatcgcgct gacgggtagc atcgacctcg tctacggaga
     2281 ccggacaggt ctttgggtcg tcgacttcaa gaccgacagg gtcgtcgccc cgtcgcgcca
     2341 cgccttccag ctagccgtgt accgcgacgc ggccgaggcc atgttcggcc gcccggcgag
     2401 agcggtcgtc cactacctac gccatggcga ggaacgcgaa atcgtcgaat cgccggatct
     2461 atcgctcctc gagggcgagt aatgtttttt tatccagttg ctccaaataa tcaaaaaaat
     2521 actagactaa gaatggatta tctgccatac tgctggaacg aagaatggca cgcggcacta
     2581 caccttcgtt acgccgcgca tccagcccga tgtctgtacc caacacggcc ccgcatccag
     2641 cgtgccgttc ttcgtgcact aaaacatagc aataaattga gcctaggcac ggcgccagcc
     2701 gccgcgcgaa aggtacgccc gtgaacgaca cctcaccgcc ggtcaagcga ccgggcttct
     2761 tctccatctt cggcttcttc ttcggtatct cggccattac cataggcggc ggctacgtca
     2821 tggtgccggt catccagcga gccctcgaga agcgcggctg gctaccggag gacgagttct
     2881 acgacctgtt cgcgaccgcc cagagcgtac ccggccccct ggcgctgaac accgccatgt
     2941 tcgccgggcg caaggtcgcc ggactacccg gcttcctggc cgccttcctc ggcatactgc
     3001 tgccgccgtt cgcggccata ctgctgctcg cggccctgct cggcgctatc ggggactggc
     3061 ccgtcgtcca gggcttcctg gacggggcct tcgcggtcgt tcccggcctg accgccgcgc
     3121 tcgcgttcaa catgatacgg aagcgccgct ggacgccgct cagggctgtc atgaccgtgg
     3181 cgtcggccgt cgcgataacg ctcgcgggca cctgggcggt accgaccttc ttcgccgtcg
     3241 tcgccgtgtc cgccttcgcc gaaaggaagg gcgcatgctg aggctggcgc tcgtattcct
     3301 caagataggc gtcgcggcct acggcggcgg ctggaccatc gtcggcataa tccggaccga
     3361 gatcgtcggg cgggcctgga tgagcgccga ggaattcgcg aagctcgtcg cgatggcgca
     3421 gatcaccccg gggccggtgg ccctcaacgc ggcgaccctc gtcggctaca ggctgcacgg
     3481 gctgctcggc gccgcggtct ccacgctggc ggtcgtcgcg gccccggtcg ccctgtccct
     3541 gctcatcggc ggcttcttcg ccaaggcgac caagagcggc gccaggctga ccgaggcgct
     3601 caagacgggt accataggcc tgatcgcgat gacggtctgg gccttcgcgc cggcggccgt
     3661 ctcgtcaggg ctgaacgtcg ccatagcgct gacggccttc gccgtctcgg ccttcaccag
     3721 gatcaacccg ctgtggatca tcctcggctc gggcgtcatc ggcgccgcga cgcgggcgtt
     3781 tttaggataa tatgcgctcg ctttatacga tagatagcgt atacttgact cattacgcgc
     3841 gcttcgacgc ttcgtggaga cgagtcgatg aataacgaac gcatcatgat agtcgaggac
     3901 gagaagatca tcgcgataga cctccagcgc aggctggagc gcttcggata cgacgtcgtc
     3961 ggaatggccg gcgaagggac cgaagccgtc gcgatggcgc tcgagctcac ccccgacatc
     4021 attctgatgg acataatgct ggccggcgac atggacggcg tcgaggccgc caaggccgtc
     4081 aaggcgtcca aggatatccc gatcatcttc ctgaccgcct acaccgacga gaggacgctc
     4141 gagcgcgcca aggaagtgga gccgtacggc tatatcctga agccgttcaa ggagcgcgag
     4201 ctctatacga cgatagacat agccctctat aagcacgaga tggccagcaa gctgacccgc
     4261 caagagcgct tgttcagcgc catcctgcag tccatcaacg acggcatcgt cgccctggac
     4321 accgacctga acgtcagctt catgaaccag gtggccgagg atatcgtcgg gacgagcgag
     4381 cgcgaggcca agggcaagca cgtcggcacc atcctatcga tcctcgaccc ggcgacgaag
     4441 agcgacatgc tcaccggcat gatcaacgtc ggcaagcgct ccaccttctt caccgacgtg
     4501 tcgctcagga acctggcggg ccagaccgtg gtgctcgacg gggcgatcac gaagatccac
     4561 gagcaggcca actcgaccga cggctacctc atcacgatgc gcgacatcac cgagctcagg
     4621 cgcctatcgg actcgctcaa ctaccagtcg agccacgaca gcctgaccgg cctctcgaac
     4681 cgcgaggaat tcagctacaa gctcagcgag atactcaaga acgtcaagat ggtcggcggc
     4741 tcgcacgccc tgctcgtgct ggacatcgac cgcttcaagg cggtcaacga cacctgcggc
     4801 accctcgccg gcgacgagct cctcaggcag atagccagcc acgtccagac caatatccag
     4861 cgcaacgacc tctcggcgcg cataggcgcc gacgagttcg ccatcatcct gatgaactgc
     4921 gagctcgagg acacgatcaa cgtgggcaac cgcctccagg gcgcggtaca gcgccagcgc
     4981 ttccaatggc aggcgaccac gtttcccatc accctgagca tcggcatcgt gccgatcgac
     5041 cgcgacgccg gcgacatcca cgcggtgctc gcggccgcgg acgattcctg ccacatctcc
     5101 aaggaggaag gcggcaacaa gataacgatc ttcagcgccc aggacagcaa gttcaagcgg
     5161 cggcgcggcg acatggagtg gatcggcaag atcaaccggg ccgtcgagga gaaccgcttc
     5221 ctgctctact accagcccat cgagccgctc ggcgcgggcg ccgagccgaa gatcgagata
     5281 ctactgcgcc tggtcaacga ggacggcagc atctccgggc ctaccgactt catcccggcg
     5341 gccgagcgct acaacctgat gcctcaggtc gaccgctggg tgctcgagaa ctcgatgcgg
     5401 gcgttcaggc tattgtccga ccaggggtcg ccgctggccg accggatctt ctcgatcaac
     5461 ctctcgggcc cggcgctgct cgacgacgcg ctgatcgaca ccatcctgta ctatctgaac
     5521 aagtactcgc tcaaggccag cgatttctgc ttcgagatta ccgagaccgc ggcgatccag
     5581 aacctgtcct acgcgacgcg cttcatgaac cgcctcaagg tcgagggctt caccttcgcc
     5641 ctcgacgact tcggcgccgg tttctcgtcc ttcggctacc tacgtacgct acccgtcgac
     5701 tacatcaaga tcgacggctc gttcgtgcag aacatcgacg agagcccggt cagttacacg
     5761 atggtcgaat cgatcaactc gatcggccac gtgatgggcc tcaagacgat agccgaattc
     5821 gtcaagggcc cggcgatcaa ggacaagctg gtggagctgg gcgtcgacta cggccagggt
     5881 taccacttct ccaagccgct gccgctgttc gactaggtca gcggccgggc ccggaccagt
     5941 ctcgacacac cgagccgacg tcccgctcga agcgcggcag atcgtcgagg ctcttgaccg
     6001 gcgacagctc ctcgggtttg gcaatgggaa ggaggcgcgc gcgcccctcg gccgcgagta
     6061 gcgcgtcgac gttgtccagg gcgaactcgc cgcccaggtc gtccgaggca ggagcgttgc
     6121 ccggtatcgc gtagcccccg tccgggctcc agtagcgttc ccgtatcgac tcgatggcct
     6181 cgcgtagcgc ctcggcgcgg tagacgcgca ggccgacgtt ggtgtagccg gcggcgccgc
     6241 tttcgaccgc cccgcccgct ttcgcggcgc ccccccgcag cttggcgtgc ccgaagctcg
     6301 ccggtaggcc gccgggccct aggcctatcg ggtaggccgg cgagtcgacg agggccgccg
     6361 gcaggaggag ccccggggcc cggtcgccga gttcgcgctc cagcgcggcc atcgcggcga
     6421 gcgaggccaa ggcggtcaac gggctcgagg cgtcgccgcc gaagttcacc gtcacgtagt
     6481 cgtagccgga ccataggccg agcgtctgga acgcggcgtc gccatggccg cgcggcttac
     6541 cgccgggggc gccctgggtc gcgaagcgat acgagcctgg cgcgaagccg aacggcccga
     6601 ggatgcgctc ctcgatctcg cgctcccagc cccgcacgac gatgacgtgg tggccgaggt
     6661 cgcgtacggc cgcgagcgcg tacgaggcga tcggcgccgg gtccggcccg aagggcatgg
     6721 cgttacggac gggatagagg ccgcgcggcg ctctgggatc ggcgcccata tcccggccgg
     6781 ccgccctggc cgcctccagc gaggctaccc agcgcgagcc cgagccggcg agcatggtca
     6841 ggcaacaggt ccgcccagcg agctcgagcg ccagcccgtc cgagtcccgg actcctagcg
     6901 tcgacgcggc cgcctcaggc aggccacgac gggccgagaa ggcccgcgac aggccggtcg
     6961 tcgtcaactc gtcgaagctc atccgcccgc ccatcgccta agcctcgggc aggaaggacg
     7021 gcttcgtcgg catgacgcgg tacttgcgca cgaagccgct ggggcggtag gtcatcttgg
     7081 cctgcctgag cccctcgtcg ccgaggtcct gttcgcggtt gacctgcctg aagtagggcg
     7141 gcagggcctg ggcgaaggcc tggttgatga actggtagat gcccttatag tcgccggtcg
     7201 ccttctcgaa gtgcacggcg aacatgcggg ccttggcgat aggctcgccg agcgcgtagc
     7261 cggccggctt gccgtcgacg tagtagaccg cgccgcgcat gcccaggcgt tcgtacagct
     7321 cgagcgcttc cctggcggcc gcgtagtcgc cgtctatgcc tttctcgacc cgccacgcgt
     7381 ccaggacgcg gatagcgtcg gcgacgttcg aggcgtcgag ctccttctgc tcgtacgagt
     7441 acgagttcag gaaggcgttc accaggttgc gcttcttatg gtacgccttg cccgacagct
     7501 cggcgagatc cttacggtcg tacaggtagt cgaagttgtc gcggtcctcg tggacctcgt
     7561 agccggcggc ctcgaggcgg atgcgctcgg ccttgcactg gctctcggac atgttcttga
     7621 ggtagcagtg atcctcgaac aggcgctcga gcgtggcgtt cggcgggatg cagcacggcg
     7681 tgaagaagaa gcgctccccg cgcttctcgc cggagattac gtaggtggag ccgctgatat
     7741 gcgacgcggt gtacttgtag gtgtcccgga acaggtagag gccggagaag gtgaactccg
     7801 acacgccgtc cgctagcagg ttgagggccg ggtagaggtc gtcgcgcatg tcgacggtca
     7861 ccggggcgaa ttcagggtat tctggtattg gcatgcctac gactatatgc aaaattcccg
     7921 atagaatcaa ggaaaggccc gggccgcgat gaacgcgcgt cccgggcctt gatgaggcga
     7981 tcgccccggc tagggtttga gggagtcgat ccgatgctcg atcatcaggt cgagcttgcg
     8041 gttctcgagc tgcagggcct cgaggcgcag gcgctcgcgc tccagctcga gctccttctc
     8101 gcggacagcg caacgggcca ggtccacccg attgcggccg cgcttggcga gcaggatgaa
     8161 gccgaacacg atgcacggcg ccactatgaa gatggacacg atggccagga ctccctcgta
     8221 cggcatgctc tattccttga tcaggatgaa ttcgacgcgg cggttcttcc accggttctc
     8281 ggcgtcggtg aacggcacga ccggctcgga ggagcccagc ccccgtaccg acaggcgttt
     8341 cgggtcgacg ccgtactcga tgagcttctg catcacgagc gcggcccgat tctccgacag
     8401 cggcaggagc tccctggtct cctcccggtc gatcgccggc tggcctgccc cggtcatctt
     8461 ggcgatcgag ttggcgtggc cctccacccg gatcttgtag tccttgaagc ggttgaggat
     8521 ctgggcgatg cgcttgagca cctcggcgtt gcggtcgagg gtctcctgcg gcaggtcctt
     8581 gaagtcggcg aagtccggcc tgaacacgat cgacgggacc ttgatcttga gccggtcgcc
     8641 gtcgcggatg accaggacgt ccacggagat gagcccctcg gagaccgtct gattgcccag
     8701 ctcgtcgacg atggtcaagc ggtacgggta atcggtcgcg gcctcgacca gctcgccctt
     8761 ctgcgagcgg ccgtcccagg ccaggcgctc ggccggcttg ccgcggccgc tccacgagaa
     8821 gaagacgcgc tccgcccgct tggcgccggc gctctcgctc acggccacct cgacgatgtc
     8881 gaagcgccag gagtcgatca cgctggcgtc ggagacggcg agcgatatcc tgagctcgtc
     8941 gtcgacgcca tcgttatcgg ggctgaaata acggggcgag gtcgtcatgg cgaccttcgg
     9001 cccctcggta tcgagggaga atgtcgcgct ggaggcctgc gcgcggtcgc ccttgaggta
     9061 atcgaccgtg aagacggcgg tataggcgcc ctgcctgagc tgaccgtcgt cggacttgcc
     9121 gtcccaggcc agcttggcgg gaatggtcgc gcccttgccc gtgaacgagc ggcgcgtgac
     9181 gccgtcatcg ccgaccatgg ccagcttcca ggaatcgatg ccgtcggtca acttcacgac
     9241 caggccgaag gacatctcgt cgaacatgcc gtcgccgttc gggctgaagc cggctgcgct
     9301 ggcggtgacg aaggcctgag tctgcctggt atcgacgacc atcctctgga tggctttctc
     9361 ggtcttgttg ccggccgggt cctcggatcg cacgacgta
//
LOCUS       DAOPHV010000026         9297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_9259_length_9297_cov_4.897858, whole genome shotgun sequence.
ACCESSION   DAOPHV010000026 DAOPHV010000000
VERSION     DAOPHV010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9297
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9259_length_9297_cov_4.897858"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLQ29_01370"
     CDS             <1..364
                     /locus_tag="PLQ29_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85321.1"
                     /translation="FHRMLTTIVGNQLLTQVHYLLRDVLKETMKRITVKVGRENGLLY
                     HRRIIEAIEAEGLLSGFRGRTASDLYLYLVKHWDELKRRYGLHYPVSAAARDFRDRYG
                     RGFLKDAKAFLGSLFKKR"
     gene            550..1455
                     /locus_tag="PLQ29_01375"
     CDS             550..1455
                     /locus_tag="PLQ29_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPG85322.1"
                     /translation="MAKNMKSQPKRCAEPFQGRLVVITGATSGVGFHTARKYASMGAR
                     VLMINRNEDKSERVRKELSDEFGASVDYIIADLAVLADIQRAGRYLADLQAPIDALIH
                     NAGLHLTTRSETIDGLEVNFVLHYLSTFIITSMLLDKYRRDGTGRILFVGSEAYRFAA
                     WGLDLDDLQWERRRYAGIKAYGAGKLAQLLSMHRFAAELAPHGVTVNAMHPGMVRTET
                     GKDNGRLYRWYKRNLIDGRSMSPEVSAEALYYLGASSEPARTSDTFFHLTTEEELAPP
                     ARDLAAAEALWARTVELLRRKGVTA"
     gene            complement(1516..3327)
                     /locus_tag="PLQ29_01380"
     CDS             complement(1516..3327)
                     /locus_tag="PLQ29_01380"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HPG85323.1"
                     /translation="MRKAAFALLASLDFSSMAGAQGRNIVILHSYQYDYEWTRLEHAG
                     IMSAFEDSGEGALIIKTDYLDAKRSWNPAKARNAADYLRDTYRDYSADVVVATDNDAF
                     TFLGEYGDGIFGDAPVVFCGVNDFEESMLAGREGRWTGVAEYIDYDSTLAALRSMFPK
                     VDEVRVLVDDSTTGISSRADFLSSIERNRFPLSVTFFAASDFLSALAEAESLPPTTAV
                     ILFTYARDPAYSDRPVSDIVSRLTAACTGPVFSFWDFNFGFGIVGGALTSGFSQGREA
                     GLMALRVLRGESPGSIPVTEARRTPLTFDWRALKRFKVDERNLPAGSVIEFAPQSLYE
                     RDPRTFLFIVVISIAALLVISFLTVALTLVIRARSKLQKNERLLTAALEEQRSLLREV
                     HHRVNNNFQIISSLLSLQASGAAEPRTREALLEISDRVRSMALVHERLYATMNFSSID
                     FIEYLRLLCEALSAERPERSGRIVFRIAPGSLLLDMETAVPLGLICNELIVNSIKYAF
                     PDGRQGLISVFLTEEPDGVGTLTLADDGVGLPPGVSFDSDQSLGFQLIHALACQAGET
                     VSARPVEWGARFDIRIAGRAQAGCQDVAALDSRPISG"
     gene            complement(3332..4258)
                     /locus_tag="PLQ29_01385"
     CDS             complement(3332..4258)
                     /locus_tag="PLQ29_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85324.1"
                     /translation="MDGSTTGLPGGSLAFAKLCGAGNDFIVLDGEAARSAPGAETIRL
                     LCDRRYGVGADGLMLLRRGSGAGAGGDDAAFEVDFYNADGSGGMLCGNGARCALERAR
                     ILGWIEPDRAVGFRFAGRDYRGSSLGEGMARFELDPRVRMDAPRLVRVGDLSLSGRFV
                     DVGSPHFVVDVAAILDGDGRRAFGSLDDVPLDALGQAVRNHPDFGAAGVNANVTELLG
                     ATLFVRTFERGVEAETLSCGTGSASSALVRWRDGELRPPVTVVARSGDELAVDFEAAS
                     DGSLTRLSLSGPAIQTFEGRVVALPLYGQAVY"
     gene            4319..5866
                     /gene="cls"
                     /locus_tag="PLQ29_01390"
     CDS             4319..5866
                     /gene="cls"
                     /locus_tag="PLQ29_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPG85325.1"
                     /translation="MKLRVKPTILRAAILLILIAIQLALMAATIWTTSQASALLNGVL
                     SLVSLLVVLYIVSSNADPSYKLTWSVLILIVPVFGGLFYLFFGLQPSSRKLRRRIAGL
                     DISARPYRRQEGGVVEAIGAMRDDFGRQAAYLSRTAGFPAYDGSSAEYLTSGEAKFEQ
                     LKAELAKAERFIFLEYFIIEAGLMWDSILAILEEKAAAGVEVRVLYDDIGCLLTLPSG
                     YAESLRRKGIHCAAFNPAQGYPSAILNHRDHRKIVVIDGRVAFTGGVNLADEYINEVE
                     KYGHWKDAAVVLRGKAVDGFTLMFLGMWNLETNESDDYPRYLAGRATSSPADAGAARG
                     DYIQPYADSPMDHENVGASVYLNIVNAARRYVYITTPYLIVDHALMTALCLAAKSGVD
                     VRVITPRHWDKLVVHMTTRSYYDELVSAGVRVFEYLDGFMHAKTFVSDDAVAVVGTTN
                     MDFRSLYLHYECGAVLYGGKAVLDVRDDFERTLGRCDAVSPGEYRPRGVLGRLCLQVL
                     RLFAPLL"
     assembly_gap    5961..6060
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6063..7598)
                     /locus_tag="PLQ29_01395"
     CDS             complement(6063..7598)
                     /locus_tag="PLQ29_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HPG85326.1"
                     /translation="MAGTKAFDNETYIAEQASCIRERMRRFDGRLYLEFGGKLLADFH
                     AARVLPGYDPNVKIRLLKELERDAEIVLCIHAGAIEHRKIRADYGITYDADAMKLIDD
                     LGEWGLSVSAVVITRWEGQPAAKAFRTKLENRGVAVYVHEPTKGYPTDVDRIVSDEGY
                     GANPRIATTKPLVVVTGPGPGSGKMATCLSQVYHDRRIGERAMYAKFETFPVWNLPLK
                     HPVNLAYEAATLDLRDFNLIDPFHLEATGEVAVNYNRDVESFPILKGILDRITGEPDA
                     YRSPTEMGVNRVGFAIVDDEAAREAARQEAARRYLRCAAEYMMGMADKAALDKADLLL
                     KELGIGPEYRKVVPAARAAAAEARERAEADPGAAAGGVAACGAALELPSGEIVSGKNS
                     KLLHAATAAVLNAVKALAGVPDQIHLLSPLVIESIARLKREVLGRKSASLDVDEALIA
                     LSISATLTPMVQACVDHLHELRGCQMHLSHLPTPGDEAGLRKLGVNLTADPAYATKRL
                     FVE"
     gene            7766..8746
                     /locus_tag="PLQ29_01400"
     CDS             7766..8746
                     /locus_tag="PLQ29_01400"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HPG85327.1"
                     /translation="MIRNLWYAVLESKEIPKNKPVGFMRLGERMVFWRDEAGEAHCCF
                     DKCAHRGASLALGDLVDGHARCPFHGLEYDGSGRCVRIPANGRDAPVPPAFRMRSYPV
                     FEGWGWVFIWWGDGEPDPAEPPYFEDLKGFTRWGSVQDPWNNHYTRVAENQIDVAHLP
                     FVHHNTIGRGNKTLVEGPGVEWVSDRLFFQYVYNKVDDGSKPRGPNEVPVPDPSRDYK
                     IEFLMPNLWENRISEKLRVSAAFVPVDDEHTLLYLRFYPGFMPFPPLAWLIGKIGSRM
                     NLVIAHQDRRVVNTQLPKSDGTGTGENLFRGDKPIMEFRKKRQELRKALG"
     gene            8811..>9297
                     /locus_tag="PLQ29_01405"
     CDS             8811..>9297
                     /locus_tag="PLQ29_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85328.1"
                     /translation="MTGAFTLAIGERLYGLRLAGGRLGSRPVAGSEAATGRLVRLEPR
                     GPGGPVPGGAAAGVDWDAAEDLLGDAGFWAALSPEDAASLADIAGRVAAELEGGRPGA
                     EAMAAACALELVVSAARLARSYPSARRPWREPDGVWSVDDAVRYIEANYAESFSLDWF
                     VA"
BASE COUNT         1624 a         3063 c         3009 g         1501 t
ORIGIN      
        1 gtttcaccgc atgctgacga ccatcgtcgg aaaccagttg ctcacgcagg tgcactacct
       61 gctgcgcgac gtgctcaagg agacgatgaa gcgcatcacg gtcaaggtcg ggcgcgaaaa
      121 cggcctgctg taccaccgca ggatcatcga ggccatcgag gcggaagggc tactgtcggg
      181 cttccggggg cgtaccgcga gcgacctcta cctgtacctg gtcaagcatt gggacgaact
      241 caagcgccgc tacggcctgc actaccccgt atcggcagcc gcccgcgact tcagggaccg
      301 ctacggccgc ggattcctga aggacgccaa ggccttcctt ggctcgctat tcaagaagcg
      361 ctgatccttc tgaatggcgc ttcaggcgag tgcaaacggg cgcggaatcg ctacaggatc
      421 caaaccaatc ctcatacctg tatcagacgt agcggataca gtagaatctc gtaacgcgag
      481 gagcgcagcg acatgaaccc agcgatacgg cgatattggc ctcagtatac gtggtcgaac
      541 gtgctcgcga tggcgaagaa catgaagagc cagcccaaac gctgcgctga accgttccaa
      601 ggacggctgg tggtcatcac tggcgccacc tcgggcgtcg gtttccatac ggcgcgtaag
      661 tacgcctcca tgggcgctcg agtgctcatg atcaacagga atgaggataa gtccgagcgc
      721 gtgcgcaagg agctgtccga cgagttcggc gcgtccgtgg actacatcat cgcggacctc
      781 gccgtcctcg cggacatcca gcgggccgga cgctacctag cggaccttca agcgccgata
      841 gacgcgctca tccacaacgc cggcctgcac ctgaccacgc ggtcggaaac gatcgacggc
      901 ttggaggtca acttcgtact gcattacctc tcgacgttca tcatcacgtc gatgcttctg
      961 gataaatacc ggagggatgg gacgggacgg atactcttcg tcggctccga ggcgtaccgt
     1021 ttcgcggcct ggggcctcga cctggacgac ctgcagtggg aacgacggcg ctacgccggg
     1081 atcaaggcgt acggagccgg taagctggcg cagctattgt cgatgcaccg cttcgccgcc
     1141 gagctcgcgc cccacggcgt gaccgtgaac gccatgcacc cgggcatggt ccgtacggag
     1201 acgggcaagg acaacggcag gctctaccga tggtacaaga ggaacctcat cgacggcaga
     1261 tcgatgtcgc ccgaggtttc ggctgaagcg ctgtactatc tcggcgcctc gagcgaaccg
     1321 gcccggacga gcgatacgtt cttccacttg acgacggagg aggaactggc gcctccggcc
     1381 cgggatctcg ccgcggcgga ggcgttgtgg gcgcggaccg tcgagctgct tcgccgcaaa
     1441 ggagtgacgg cgtaatgtac gaggcttacg tcgccatggg aggcgtcatg gcgggacggg
     1501 ccgtagcgac gtcggtcatc cgctgatcgg gcgggaatca agcgcggcaa cgtcctgaca
     1561 gccggcctgg gcgcgcccgg cgatacggat atcgaagcgc gcgccccact ctacggggcg
     1621 cgccgacacc gtctcccccg cctgacaggc cagggcgtga atcaactgga aacccaggct
     1681 ctgatcgctg tcgaagctca ccccaggcgg caggcctacc ccgtcgtccg cgagcgtcag
     1741 cgtgccgacg ccgtccggct cttcggtcag gaagaccgat ataagccctt gtcggccatc
     1801 cgggaacgcg tactttatcg agttgacgat gagctcgttg catatcaagc cgagaggaac
     1861 agctgtttcc atgtccagga gcagcgatcc aggcgcgata cggaaaacga tgcgcccgga
     1921 ccgttccggg cgttcggcgc tcagagcttc gcataacagg cggaggtact cgatgaagtc
     1981 gatgcttgag aaattcatcg tggcgtacag tcgttcgtgg accagcgcca tactacgcac
     2041 ccgatcggat atttcgagca gcgcctcgcg cgtacgcggt tccgcggctc ccgaggcctg
     2101 caggctaagc agactggata tgatctgaaa attattattg acgcggtggt ggacctcccg
     2161 tagcagggac cgttgctcct cgagcgcggc cgtcaggagc cgctcgttct tctggagctt
     2221 ggacctggcc cggatcacca gggtgagcgc gacggtcagg aacgatatga cgagcagagc
     2281 cgcgatcgag atgaccacga tgaacaagaa cgtccgcgga tcccgttcgt acaggctctg
     2341 cggcgcgaat tctatcactg aacccgctgg caggttccgt tcgtcgacct tgaagcgctt
     2401 gagcgcccgc caatcgaacg tcagcggcgt acgacgggct tcagtaaccg ggatggagcc
     2461 gggcgactct ccgcgcagga cgcgcaacgc catgagcccc gcctcgcggc cttgggagaa
     2521 gccggaggtc agcgcgccgc cgacgatgcc gaagccgaag ttgaaatccc agaacgagaa
     2581 caccggcccc gtgcatgcgg cggtcaagcg cgagactatg tccgagacgg gccggtcgga
     2641 gtaggccgga tccctcgcat aggtaaacag gataaccgcg gtcgtaggcg gcagggactc
     2701 cgcctcggcg agcgccgaca aaaaatcact cgccgcgaag aaggtcacgg aaagcgggaa
     2761 tcggttcctc tctatcgagg aaaggaaatc cgcccgcgaa ctgattcccg tggtactgtc
     2821 gtccacgagg acgcggacct cgtcgacctt ggggaacatc gagcgcaggg ccgcgagcgt
     2881 cgaatcgtag tcgatatact cggccacgcc cgtccagcgc ccctcgcgtc ccgccagcat
     2941 cgactcctcg aagtcgttga cgccgcagaa tacgacgggc gcatctccga aaatgccgtc
     3001 gccgtactcg ccgaggaacg tgaatgcgtc gttgtcggtg gcgacgacga cgtccgccga
     3061 gtagtcgcgg tacgtatcgc gaaggtagtc ggccgcgtta cgcgccttgg cgggattcca
     3121 cgatcgcttg gcgtcgaggt agtccgtctt gatgatcaga gcgccttcgc cggaatcttc
     3181 gaaggccgac atgatgccgg cgtgctcgag gcgcgtccat tcgtagtcgt actgatagga
     3241 atgcaggatg acgatattcc tgccctgggc tccggccatc gaagaaaagt ctagggatgc
     3301 gagcagggcg aacgcggctt ttctcatgcc cctagtatac agcctgcccg tataacggca
     3361 aggctaccac gcgtccctcg aaggtctgaa tcgccggacc ggagagcgac agcctggtca
     3421 gcgagccgtc cgacgcggct tcgaagtcga cggcgagctc gtcgccgctc cgcgctacca
     3481 cggtgacggg cggccgtagc tcgccgtcgc gccagcggac gagcgcgctg gaagcggaac
     3541 cggtgccgca cgacagggtt tcggcctcga cgccgcgctc gaaggtacgg acgaacagcg
     3601 tagctccgag gagctcggta acgttggcgt tgacgccggc ggcgccgaaa tcggggtggt
     3661 tccgcacggc ttggcccagt gcgtcgagcg gtacatcatc gaggctgccg aaggcgcggc
     3721 ggccgtcgcc gtccagaatg gccgcaacgt cgacgacgaa gtgcggactg cccacgtcga
     3781 cgaagcggcc gctcagggac aggtcgccga cgcgcacgag gcgcggggcg tccatgcgga
     3841 cgcgagggtc gagctcgaag cgggccatcc cctcgccgag ggaggagcct ctgtagtcgc
     3901 ggccggcgaa gcggaagccg acggcccgat cgggctcgat ccagcctagg atcctggccc
     3961 gctccagggc gcagcgtgcg ccgttaccgc agagcatgcc gcccgagccg tcggcgttgt
     4021 agaagtcgac ctcgaaggcc gcatcgtcgc ctccggcgcc ggcgcccgag ccgcgccgga
     4081 ggagcatgag gccgtcggcg ccgacgccat agcggcggtc gcagaggagc cgtattgtct
     4141 cggcccccgg cgctgacctc gccgcctcgc cgtcgagaac gatgaagtcg ttaccggccc
     4201 cgcatagctt ggcgaaagcc agagagcccc cagggagccc cgtcgtcgat ccgtccatgg
     4261 acagggagta tagcacccaa gcttcggaac acgatacaat acaggcggag aacgacaggt
     4321 gaaactacgc gtaaagccga ccatactgcg ggccgccata ctcttgatcc tgattgcgat
     4381 tcagttggcg ctgatggcgg cgacgatctg gacgacgagc caggcctccg cgctactcaa
     4441 cggcgttcta tcgctcgtca gcctgctcgt ggtgctgtac atcgtcagct ccaacgccga
     4501 tccttcctac aagctcacct ggtcggtact catcctgatc gtgccggtct tcgggggcct
     4561 cttctacctc ttcttcgggt tacagccctc gtcgcgcaag ctgcgcaggc gcatcgccgg
     4621 actggacatc tcggccaggc cgtataggcg ccaggagggc ggcgtcgtcg aggccatagg
     4681 cgcgatgcgc gacgacttcg gcagacaggc cgcctacctg agccgaacgg ccggtttccc
     4741 ggcctacgat ggctcctcgg ccgagtacct gacctccggt gaggccaagt tcgagcagct
     4801 caaggccgag ctggccaagg ccgagcgctt catcttcctc gagtacttca ttatcgaagc
     4861 cggcctcatg tgggactcga tcctcgcgat actcgaggaa aaggctgccg ccggcgtcga
     4921 ggtgcgagtg ctctacgacg acatcggctg cctcctgacc ctgccgtccg gttacgccga
     4981 gagcctgcgc cgcaagggca tccactgcgc cgcgttcaac ccggcccagg gctatccatc
     5041 ggccatcctg aaccaccgcg accaccgcaa gatcgtcgtg atcgacggcc gcgtcgcgtt
     5101 taccggcggc gtcaacctgg cagacgaata tatcaacgag gtagaaaagt acgggcactg
     5161 gaaagacgcc gccgtcgtcc tgcgcggcaa ggccgtggac ggcttcacgc tcatgttcct
     5221 tgggatgtgg aacctggaga cgaacgaatc ggacgactac cctcgctacc tggcggggcg
     5281 cgccacctcg tcaccggccg acgcgggggc cgcgcgcggc gactatatcc agccttacgc
     5341 cgacagcccg atggaccacg agaacgtcgg cgcctcggtc tacctgaaca tcgtcaacgc
     5401 ggccaggcgc tacgtctata tcacgacgcc ctacctgatc gtcgaccacg ccttgatgac
     5461 ggccctctgc ctggcggcca agagcggcgt cgacgtgcgc gtcatcacgc cgaggcactg
     5521 ggacaagctc gtggtgcaca tgaccacacg ttcctactac gacgagctgg tatcggccgg
     5581 cgtcagggtc ttcgagtacc tggacggctt catgcatgcc aagaccttcg tgtcggacga
     5641 cgccgtcgcg gtcgtcggaa cgaccaacat ggacttccgt agcctatacc tgcactacga
     5701 gtgcggcgcc gtgctctacg gcggcaaggc cgtcctcgac gtgcgcgacg acttcgagcg
     5761 caccctcggg cgctgcgacg cggtctcgcc gggcgagtat cggccgcgcg gcgtcctggg
     5821 ccggctctgc ctacaggtac tcaggctctt cgcgccgtta ttatgacggc gcgacgccgt
     5881 tattatgacg gcgcgacgcc gttactatga cggcgcgacg ccgttactat gacggcgcga
     5941 cgccgttact atgacggcgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 gcctattcta cgaacagccg cttcgtcgcg taggccgggt cggcggtcag gttgacgccg
     6121 agcttacgca ggcccgcctc gtcgccgggc gtcggcaggt gggacaagtg catctggcaa
     6181 ccgcgcagct cgtgcaggtg gtcgacgcag gcctggacca ttggcgtcag ggtggcactg
     6241 atcgacagcg ctatgagcgc ctcgtccaca tccaggctgg cgctcttgcg gccgagcacc
     6301 tcgcgcttga gcctggctat cgactcgatg accagcggcg acaggagatg gatctgatcg
     6361 ggcacgcccg ccagcgcctt gacggcgttg agcacggcgg ccgtcgctgc gtggagcagc
     6421 ttggagttct tgccgctgac gatctccccg gaaggcagct cgagggccgc cccgcaggcg
     6481 gcgacgcctc cggccgcggc accgggatcg gcctcggcgc gctcgcgggc ctcggccgcg
     6541 gcggccctgg cggccggcac caccttccgg tactccggcc cgatgcccag ctccttgagg
     6601 agcaggtcgg ccttgtcgag ggccgccttg tcggccatgc ccatcatgta ctcggcggcg
     6661 cagcgcaggt aacggcgcgc cgcctcctgc ctggcggcct cccgggccgc ctcgtcgtcg
     6721 acgatcgcga agccgacgcg gttgaccccc atttcggtag gcgagcggta ggcgtccggc
     6781 tcgccggtga tgcggtccag gatgcccttg agtatcggga acgactcgac gtcgcggttg
     6841 tagttgaccg ctacctcgcc ggtcgcctcg aggtggaagg ggtcgatcaa gttgaaatcc
     6901 cgtaggtcga gggtcgccgc ctcgtaggcc aggttgactg ggtgcttgag cggcaggttc
     6961 cagaccggga aggtctcgaa cttggcgtac atcgcccgct ctccgatgcg gcggtcgtgg
     7021 tagacctgcg acaggcaggt ggccatcttg cccgagcccg gccccgggcc ggtcaccacg
     7081 acgaggggct tcgtcgtcgc tatccgcggg ttggcgccgt agccctcgtc ggagacgata
     7141 cggtccacgt cggtcgggta gcccttggtc ggctcgtgca catagacggc cacgccgcgg
     7201 ttctcgagct tggtgcggaa ggccttggcc gccggctgac cctcccagcg cgtgatcacg
     7261 accgccgaga ccgataggcc ccactcgccc aggtcgtcga tcagcttcat cgcgtcggca
     7321 tcgtaggtga tgccatagtc ggcgcggatc ttgcggtgct cgatcgcccc ggcgtggatg
     7381 cacagcacga tctccgcgtc gcgctccagt tccttgagaa gccggatctt gacattcggg
     7441 tcgtagccgg ggagcactcg cgccgcgtga aagtcggcca ggagcttgcc gccgaactcc
     7501 aggtacagcc gcccgtcgaa gcgcctcatg cgctcccgga tgcacgacgc ctgctcggct
     7561 atgtatgtct cgttgtcgaa cgccttggtt cccgccatcg atggagcctc cctgacggga
     7621 cgacagtcta caccggaacg tagccggaac gccagataac ggccgcgagg cgccagaaca
     7681 cgcggcctgc gatttcgtcc ttgcatgccc gaattcgccg gattatagta ccggcaccat
     7741 gagctgaacc tacaggagac ggccgatgat cagaaaccta tggtacgcgg tgctggaatc
     7801 caaggaaatt cctaagaaca agccggtcgg cttcatgcgc ctcggggagc gcatggtctt
     7861 ctggcgcgac gaagccggcg aggcccattg ctgcttcgac aagtgcgcgc accggggcgc
     7921 gtccctcgcg ctcggcgacc tcgtcgacgg ccacgcccgc tgccccttcc acggactcga
     7981 gtacgacggc tcgggccgtt gcgtcaggat acccgccaac ggcagggacg cgccggtgcc
     8041 gccggcgttc aggatgcgct cctacccggt gttcgaaggc tggggctggg tgttcatctg
     8101 gtggggcgac ggcgagcccg acccggccga gcctccgtac ttcgaggacc tcaagggctt
     8161 cacgcgctgg ggcagcgtcc aggacccgtg gaacaaccat tacacccgcg tcgccgagaa
     8221 ccagatagac gtcgcgcacc tccccttcgt ccaccacaac accataggcc gcggcaacaa
     8281 gaccctcgtc gagggcccgg gcgtcgaatg ggtatccgac cggctgttct tccagtacgt
     8341 ctacaacaag gtcgacgacg gctccaagcc ccgtggcccg aacgaggtgc cggtgcccga
     8401 cccgagccgc gactataaga tcgaattcct catgcccaac ctctgggaaa accgcatctc
     8461 cgagaagctc cgcgtgtcgg ccgccttcgt gccggtcgac gacgaacaca ccctgctcta
     8521 cctgcgcttc tatcccggct tcatgccctt cccgccgctg gcctggctca tcggcaagat
     8581 aggcagccgc atgaacctgg tcatagccca ccaggaccga cgcgtcgtca atacgcagtt
     8641 gcccaagtcc gacggcacgg gcaccggcga gaacctgttc cgcggcgaca agcccatcat
     8701 ggagttccgc aagaagcgtc aggagctccg gaaggctcta ggttgacgat agggcctgcc
     8761 cccgagccgg gccgttcggc ccgccccccg gccccgcggg ccggggcgtc atgacgggcg
     8821 cgttcacgct cgccatcggc gagcggctct acggtctccg gttagccggc gggcgcctcg
     8881 gctcgcgccc ggtcgccggt tccgaggccg cgacgggccg gctcgtacgg ctcgagccgc
     8941 gcgggcccgg cggccccgtg cccggaggag cggcggccgg cgtcgactgg gacgccgccg
     9001 aggacctgct cggcgacgcg gggttctggg ccgccctgtc gcccgaggac gccgcgtcgc
     9061 tcgcggacat agccggccgc gtcgccgccg agctcgaggg cgggcggccg ggagccgagg
     9121 ccatggcggc cgcctgcgcg ctggagctgg tcgtgtcggc cgcccgcctg gcccgcagct
     9181 acccgagcgc ccgccggccc tggcgcgagc ccgacggcgt ctggtcggtc gacgacgcgg
     9241 tccgctacat cgaagccaac tacgccgagt ccttcagcct ggactggttc gtcgcca
//
LOCUS       DAOPHV010000027         9247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_9335_length_9247_cov_3.414600, whole genome shotgun sequence.
ACCESSION   DAOPHV010000027 DAOPHV010000000
VERSION     DAOPHV010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9247
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9335_length_9247_cov_3.414600"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLQ29_01410"
     CDS             <1..462
                     /locus_tag="PLQ29_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPG85329.1"
                     /translation="GFQPIAGYNYGAKRFDRVRQSLKTTVLTALAVSLAGYAFMMLAP
                     RAAIGLFTADAELIASSARVLRVMVLFIPLASLQITGSTYFQAVGKKTQSMMLGLSRQ
                     FLLLIPIVLILPLFIGVDGVWLAFPIADLASTVITLALLARELRHLESRSA"
     gene            complement(454..1818)
                     /locus_tag="PLQ29_01415"
     CDS             complement(454..1818)
                     /locus_tag="PLQ29_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPG85330.1"
                     /translation="MKIRSQFYILVFGIVVVPLLAFAGLLAMQGGRESSVVQVPGYDE
                     VAALAGGSFDRASWERLADYMAHKPPTMEFTVLDPERRVAFSTISGHKVGDLVSDEAL
                     LGYMRETSGDYFYQIDSPVRSSDGGLLVLTRIERSERLRPPNPFSRFFSSFAVLLAAL
                     FLFSAGMAVLIARSITQSVLALDAATRRIAAGELERNVEVRGSNEITSLASSLNAMRL
                     ALKDDLARRSRFIMGVTHDLKTPLALIKGYAEAIGDGMDDGADTRERYVGIIGSKVDQ
                     LAGMIDDLIDFVRVDSGEWRERLERAPLAPFLRGLCRRLEEDAVLLGRRAESSIALPD
                     DLAVPFDERLITRALENIVNNALRYTEPGGLVRVAAWLERGADGGDRVAIEVSDDGPG
                     IGEGELPLIFDPFYRGSGSRREQGMGLGLSVVKGVVESHGWELSVRSRPGEGSAFRID
                     APLR"
     gene            complement(1815..2546)
                     /locus_tag="PLQ29_01420"
     CDS             complement(1815..2546)
                     /locus_tag="PLQ29_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPG85331.1"
                     /translation="MKAKVLIIEDVAELSDLVALYLSKEGMETRRAESAEAALAMLRA
                     VGAEAFSPDIIILDINLPGMDGFEFLGEYRKAGAAPVLIVSARDADEDIIAGLGHGAD
                     EFVTKPFSPRVLVARVRAMLRRAQEAPSARDARSIRFGPYSLDLEACVLRRGEERVAL
                     SAKEYAVLAFLAANPGRPLDPETIYSSVWKNAYGDVTAVAVYIQRLRRKLEADPSSPR
                     HIETVYGMGYRFTLDPERGPDGGGR"
     gene            2738..4003
                     /locus_tag="PLQ29_01425"
     CDS             2738..4003
                     /locus_tag="PLQ29_01425"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPG85332.1"
                     /translation="MRIPITLAVALLALAATADASAQDAFNIDEAVSIALEGNRSLAR
                     AKIDREAASRALSLAWNDLVPSLSVGASASASDGRGSVGGSATASLSLSAESLHARSQ
                     AELAYVTAELAYETARRDVELSVRTAFYALLLKQEKIRLAEQNAATARKSYEQAEARR
                     KAGVAPELDALSALVRLEKLGPALESARMDYETSLDSFRQLLGLEPGAPVTLTGSLDA
                     SPVAVRAAAAVDPAAAAVSSPSVRTMERSKASAEASLAKARRAVYSPSLSLSASYRPS
                     RTDASLSGSGSLSASVSLGLDDMLPWSDARLAVASAEDAVAKIDSQLAEASAKAVSGA
                     ASLQRTIAQAAKSLETGRLSESLAARTYALTEEAYRFGTKDLLSLQSAQDSLAEARVS
                     VSESAYALIVALLNLEYALGEPFGSLGRD"
     gene            4006..4989
                     /locus_tag="PLQ29_01430"
     CDS             4006..4989
                     /locus_tag="PLQ29_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPG85333.1"
                     /translation="MRKRNLALLALFALAIAAVVVVPKLAGSGSQAAAMPPGARPTSG
                     AGPRPSGAPGRGAASATEFSVRTVRAERTTLQDYIETNGDVVVDSTVKVYPYVAGKLV
                     SSKASLGSRVTKGQTIAEIDPSTPGSAYTLNAVYAPISGTVTSLPVSVGSTVGTATAI
                     AEIGSIDELEVEARIPERYVGVLKVGLRATVSLEAYPGVSFAATVSSVSPVVDSTSRT
                     KTIRLAFDRYDERVNAGMFAKLRLDTVAYPDCLAVASGGVLQDSTGHYLFVVKDGSTV
                     EKRRVEVGVDIDGLAAITDGLSEGELVVVEGASVLADGARIKDISSPGSGT"
     gene            4986..8087
                     /locus_tag="PLQ29_01435"
     CDS             4986..8087
                     /locus_tag="PLQ29_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPG85334.1"
                     /translation="MSVAKKVVERPVLVAIAFALITVVSLYTLSDLALDLMPDTERPV
                     LMVSASYPGASPESVEKSVTAALESALINVSGLQSLSSTSSEGSSRLELEFEYGTDLD
                     AATNDVRDKLDRIRRSLPDDASDPQIFRFSSSDMPIMRIAVSGERSSEELKVIAEDII
                     QPRLEQIDGIAQADVNGGRERIVRVELSQNRLDAYGLTITGIASKLASANVELGGGSI
                     GEGTKNYLIRTTGEYSSVDEIASTVVGTAGGYAVRLSDLATVAMGYEDESRLVYVNGE
                     PGVYVSLTKRSGSNSVKAADAVYEKLEEIRPLLPSDVSMKIVSDDTLSIRSTLSNLLS
                     SALQGALLAMAVLFLFLRSLKSTVIIGVSIPFSILVTLLCMYFAGITLNMMTMTGLIL
                     GVGMIVDASIVILENIYQYRERGAKPTIAGILGTQEMTSSIVAGNLTTVVVFVPILFF
                     KDKLGMIGMMFGDIIFTIVISIVSSLFVALLLVPVLASTYLPLTTRRERPIRNRALAA
                     MDAALGKALDLLDQAYGKALRAAVAHRPTVVILVVGCFALSLAFIPDMNINMMPNSDD
                     SSINLSVTMPLGTKLGETKAVMLAMEAYAMDELEGVETIITTVGGGMMGGGSSSNGGQ
                     LSVTLPDFSERVDDAVTMQAKLRAHFSEFPNAAFSFSMGRMRQMSGADIDVVIRTQDL
                     EVGIATAHTLIDLLEEKVPDLRDVAMDLNEGLPQVEVIIDRERAYAFGITVQAIAKEI
                     DASVDGVTATTYREAGDEYAVTLVLREADRSKLPDLENIYVQGGMGRVAVANFASLEK
                     GVGPVSISREDQSRVIHVTASITSDRRAGDVEDDIKAVIADNVVLPEGTRLSYEGSWA
                     SVMETGGYFVLIVTMALLLVFGVMAGQYESWKDPIINMFTIPLLLIGVVAVYVITGES
                     LTMFTAMGVVMLIGIVVNNGIVLVDYTNLLVRRGMPVREACVRGGASRLRPVLMTTLT
                     TVLGLLPMALSNGENSQMLKPIGLTVLGGLLSSTLITLFFIPVMYSFFNERRVRVAAK
                     ESA"
     gene            8084..8380
                     /locus_tag="PLQ29_01440"
     CDS             8084..8380
                     /locus_tag="PLQ29_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85335.1"
                     /translation="MTRIEIIANRSVQDDVVDGLESAIDGFYYTVVPVVHGRGREARM
                     LGTATWPEENFMLIAYIDDGQAEVARAVVAKVKARFPTEGLKLFAVGGASSLGS"
     gene            complement(8546..>9247)
                     /locus_tag="PLQ29_01445"
     CDS             complement(8546..>9247)
                     /locus_tag="PLQ29_01445"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605759.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HPG85336.1"
                     /translation="SLALMQVPQGDTLKGKTVSVSGFGNVSWGTCIKASELGAKVVTI
                     SGPDGYIYDPDGISTAEKHKYLEEMLVINRNSCETYAKKFPNAKFFAGKRPWEQKVDI
                     AMCCAIQNEMSGDDAQMLVKNGVKYVVEVSNMGCTPEAWKLFVEKKIPFGPGKAANAG
                     GVATSGLEMSQNSMRLAWTAEEVDQRLHAIMVNIHNTCLEAAKEFGADGNYVVGANIA
                     GFKKVADAMIAQGLV"
BASE COUNT         1546 a         3132 c         3041 g         1528 t
ORIGIN      
        1 ggcttccagc ccatcgccgg atataactac ggcgccaagc gcttcgaccg ggtcaggcag
       61 agcctcaaga ccaccgtact gacggcgctg gccgtgtcgc tggccggcta cgcgttcatg
      121 atgctcgccc cgcgcgcggc catcggcctg ttcaccgccg acgccgagct gatcgcctcg
      181 agcgcgcggg tgctacgggt catggtgctg ttcatcccgc tcgcgtcgct ccagataacc
      241 ggctcgacct acttccaggc cgtcggcaag aagacccagt cgatgatgct cggcctctcg
      301 aggcagttcc tgctgctgat accgatcgtg ctgatcctgc cgctgttcat cggcgtcgac
      361 ggagtctggc tggccttccc gatagccgac ctcgcctcga cggtgattac gctggccctg
      421 ctcgcccgcg agctgcgcca cctcgagagc cgatcagcgt agcggcgcgt cgatacggaa
      481 ggcgctcccc tccccgggcc gggagcgtac cgacagctcc cagccgtgcg actccacgac
      541 gcccttgacc accgacaagc cgagccccat gccctgttct cggcgggagc ccgagccgcg
      601 gtagaacggg tcgaagatga gtggcagctc gccctcgccg atgccggggc cgtcgtcgga
      661 tacctcgatc gctacgcggt cgccgccatc cgcgccgcgc tcgagccagg ccgcgacgcg
      721 cacgagcccg cctggctcgg tgtagcgtag cgcgttattg acgatgttct caagcgccct
      781 cgtgatcagg cgttcgtcga agggcacggc caggtcgtcg ggcagggcta tcgacgactc
      841 cgcccggcgc ccgagcagca cggcgtcctc ctcgagccta cggcagaggc cgcgcaggaa
      901 gggcgccagc ggcgcgcgct cgaggcgttc ccgccactcg cccgagtcga ctctgacgaa
      961 gtctatcagg tcatcgatca taccggccaa ctggtccacc ttcgagccga tgatgccgac
     1021 gtagcgctcg cgggtatctg cgccgtcgtc catgccgtcg cctatcgcct cggcgtagcc
     1081 cttgatcagc gcgagcggcg tcttgaggtc gtgggtcacg cccatgatga agcgagagcg
     1141 cctggccagg tcgtccttga gcgcgagccg catcgcgttg agcgacgagg cgagcgaggt
     1201 gatctcgttc gagcctcgga cctcgacgtt ccgctccagc tccccggcgg ctatgcgtcg
     1261 ggtcgcggcg tcgagcgcca ggaccgactg ggtgatggac ctggcgatca gtacggccat
     1321 ccccgcggag aacaggaaga gtgccgcgag cagtaccgcg aacgacgaga agaagcggct
     1381 gaacgggttc ggcggccgga ggcgctccga acgctctatc ctggtcagga cgagcaggcc
     1441 tccgtcgctc gatcgcacgg gcgagtcgat ctggtagaag tagtcgccgc tcgtctcccg
     1501 catgtagccg agcagggcct cgtcggagac gaggtcgccg accttatgcc cgcttatcgt
     1561 cgagaaggcg acgcggcgct ccgggtcgag cacggtgaac tccatcgtcg gcggcttgtg
     1621 ggccatgtag tcggcgaggc gctcccagga ggcccggtcg aacgagccgc cggccagcgc
     1681 ggccacctcg tcgtagccgg gcacctggac gaccgacgac tcgcgtcccc cctgcatcgc
     1741 gaggaggccg gcgaacgcga gcagcggcac gacgacgatg ccgaacacga ggatgtagaa
     1801 ctggctgcgt atcttcatcg cccgcctccg tccggcccgc gttccggatc gagggtgaac
     1861 cggtagccca tgccgtagac cgtctcgatg tgccgcggcg acgaggggtc ggcctcgagc
     1921 ttcctgcgta gccgctggat atagacggcc accgcggtca cgtcgccgta ggcgttcttc
     1981 cataccgacg agtagatggt ctccgggtcg aggggccggc cggggttggc ggccaggaac
     2041 gcgagcaccg cgtactcctt ggccgataga gcgacgcgct cctctcctcg cctgagcacg
     2101 caggcctcca ggtcgagcga gtacgggccg aagcgtatcg accgcgcgtc cctcgccgac
     2161 ggggcctcct gggcgcgcct gagcatcgct cgcacacggg ccacgagtac ccgcggcgag
     2221 aacggcttgg tcacgaactc gtcggcgccg tggccgaggc ccgcgatgat gtcctcgtcg
     2281 gcgtccctgg ccgatacgat cagcacgggg gccgccccgg ccttgcggta ctctccgagg
     2341 aactcgaagc cgtccatgcc tggcaggttg atgtcgagga tgatgatatc cggcgagaac
     2401 gcctcggcgc cgacggcccg gagcatggcg agggcggcct cggcggactc ggcccgccgg
     2461 gtctccatgc cttccttgga caggtacaga gccacgaggt ccgacagctc cgccacgtct
     2521 tcgatgatca gtaccttcgc cttcatgctc accccacggt tcggagtata cgcgcggccc
     2581 gagcgccggg caacgccgag cggcggcgat gcctccgcct ataacaaaaa attatgtgcc
     2641 tttcatggcg cgtctatagg ccgcgaaccg gtttggacgt atgctcaggc tcgacgatag
     2701 ggtatcctcg gaacgatagg atgacatgga gggatagatg cgaataccga taacgctggc
     2761 cgtggccctg ctcgcgctcg cggccaccgc cgacgcgagc gcccaggatg ctttcaacat
     2821 cgacgaggcc gtctcgatcg cgctcgaggg caataggagc ctggccaggg ccaagatcga
     2881 tagggaggcc gcttccaggg ccctgtcgct ggcgtggaac gacctcgtac cgtccctatc
     2941 ggtcggcgcc tcggccagcg ctagcgacgg ccggggaagc gtcggcggct ccgcgaccgc
     3001 gagcctgagc ctgtccgccg agtcgctcca tgcgcggtcc caggcggagc tggcctacgt
     3061 gacggccgag ctggcgtacg agacggccag gcgcgacgtc gagctgtcgg tacggaccgc
     3121 cttctacgcg ctgctcttga agcaggagaa gatacgcctg gcggagcaga acgcggcgac
     3181 ggccaggaag agctacgagc aggccgaggc caggcggaag gccggcgtgg ccccggagct
     3241 cgacgcgctg tcggccctcg tcaggctcga gaagctcggg cctgcgctcg agagcgccag
     3301 gatggactac gagacgtcgc tcgactcgtt caggcagctg ctcggcctcg agcccggcgc
     3361 gcccgtgacg ctgaccggct cgctcgacgc gtcgccggta gcggtacggg ccgccgccgc
     3421 cgtcgatccc gcggccgccg ccgtctcgtc gccgtcggtt agaaccatgg agcgatccaa
     3481 ggccagcgcg gaggccagcc tcgccaaggc caggcgcgcg gtgtactcgc cgtcgctgag
     3541 cctatcggcc tcgtataggc cgagtcgtac cgacgcgtcc ctgagcggct cgggctccct
     3601 gtcggcctcg gtcagcctgg gcctcgacga tatgctgccc tggtcggatg cgaggctcgc
     3661 cgtagcctcg gccgaggacg ccgtggccaa gatcgacagc cagctggccg aggcttcggc
     3721 caaggccgtc tcgggcgccg cctcactcca gcgcacgata gcccaggcgg ccaagtcgct
     3781 cgagacgggg cgcctgagcg agtcgctggc cgcgcggacc tacgccctga ccgaggaggc
     3841 ctaccgcttc ggaaccaagg atctcctgtc gctccagagc gcccaggaca gtctggccga
     3901 agccagggta tccgtatcgg aaagcgcgta cgcgctgatc gtcgctctac tgaaccttga
     3961 atacgcgctg ggcgagccgt tcggctcgct cgggagggat tgaacatgag gaaacgcaac
     4021 ctagccttac tcgcgctgtt cgcgctcgcc atcgcggcgg tcgtcgtcgt gccgaagcta
     4081 gccggctcag ggagccaggc ggccgcgatg ccacccgggg caaggccgac gagcggcgcc
     4141 ggcccccgtc cttccggagc gccgggccga ggcgccgcct cggcgaccga gttctcggtg
     4201 cgcaccgtca gggccgagcg gacgaccctg caggactata tcgagacgaa cggcgacgtc
     4261 gtcgtcgact ccaccgtcaa ggtctacccc tacgtcgccg gcaagctggt atcgtcgaag
     4321 gcgtccctcg gatcccgcgt gacgaagggc cagacgatag ccgagatcga cccctcgacg
     4381 cccggctccg cctatacgct caacgcggtc tacgcgccga taagcggcac ggtgacctcg
     4441 ctgccggtat cggtcggctc gaccgtcggt accgccacgg ccatagccga gataggctcg
     4501 atcgacgagc tcgaggtcga ggccagaata ccggagcgct acgtcggcgt gctcaaggtg
     4561 gggctcaggg cgacggtatc gctcgaggcc taccccggcg tcagcttcgc cgcgaccgtg
     4621 tcgagcgtgt cgccggtcgt ggactcgacc tcgaggacga agaccatccg cctggccttc
     4681 gaccgctacg acgagcgcgt caacgccggc atgttcgcca agctcaggct cgacacggtg
     4741 gcctaccccg actgcctggc cgtcgcgtct ggcggcgtgc tccaggattc gaccggccat
     4801 tacctcttcg tcgtcaagga cggctcgacc gtcgagaagc gtcgcgtcga ggtcggcgtc
     4861 gacatcgacg gcctcgcggc gatcaccgac ggactgtccg agggcgagct cgtcgtcgtc
     4921 gagggcgcgt cggtgctagc cgacggcgcc aggatcaagg atatttcgtc tccggggtcc
     4981 ggcacatgag cgttgccaag aaagtcgtcg aacggccggt cctggtcgcg atcgccttcg
     5041 cgctaattac ggtcgtcagc ctctacaccc tatccgacct ggctctcgac ctgatgccgg
     5101 acacggagcg gccagtcctg atggtcagcg ccagctaccc cggggcgagt ccagagtcgg
     5161 tcgagaaatc cgtcaccgcc gcgctcgaga gcgcgctgat caatgtctcc ggcctccaga
     5221 gcctctcgtc gacctccagc gagggttcct ccaggctcga actcgaattc gagtacggca
     5281 ccgacctcga cgccgcgacg aacgacgtgc gcgacaagct ggaccggata cgccgtagcc
     5341 tgcccgacga cgcgtccgac ccgcagatct tccggttctc gtcgagcgac atgcccatca
     5401 tgcgcatagc cgtctcgggc gagcgtagct cggaggagct caaggtcata gccgaggaca
     5461 tcatccagcc caggctcgag cagatcgacg gcatcgcgca ggcggacgtc aacggcggcc
     5521 gcgagcgcat cgtacgcgtc gagctgtccc agaaccgcct ggacgcctac gggctgacca
     5581 tcaccggcat cgcctcgaag ctggctagcg ccaacgtcga gcttggcggc ggtagcatcg
     5641 gcgaggggac caagaactac ctgatccgca cgaccggcga atactccagc gtcgacgaga
     5701 tagcctcgac cgtcgtcggc acggcgggcg gctatgccgt ccgcctgtcc gacctcgcca
     5761 cggtggcgat gggctacgag gacgagagcc gcctcgtcta tgtcaacggc gagccgggcg
     5821 tctacgtgtc gctgaccaag cgctcgggct cgaactccgt caaggccgcc gacgccgtct
     5881 acgagaagct cgaggagatc aggcccctcc tgccctccga cgtgagcatg aagatcgtct
     5941 cggacgacac gctatccatc aggagcacgc tgtcgaacct cctgtcgtcc gccctgcagg
     6001 gcgccctgct cgcgatggcc gtcctgttcc tgttcctcag gagcctcaag agcaccgtca
     6061 tcatcggcgt ctcgataccc ttctccatcc tggtcacgct gctgtgcatg tacttcgcgg
     6121 gcatcaccct caacatgatg acgatgaccg gcctgatcct cggcgtcggc atgatagtcg
     6181 acgcgtccat cgtcatcctc gagaacatct accagtaccg ggaacgaggg gccaagccga
     6241 cgatagccgg catcctcggc acccaggaga tgacctcctc gatagtagcc ggcaacctga
     6301 cgaccgtcgt cgtcttcgtg cccatcctgt tcttcaagga caagctcggc atgataggca
     6361 tgatgttcgg cgacatcatc ttcaccatcg tcatctcgat cgtctccagc ctcttcgtcg
     6421 ccctcctcct cgtgccggtg ctcgcgagta cctacctgcc gctgacgacc cgtagagaga
     6481 ggccgatcag gaaccgggcc ctcgcggcca tggatgccgc gctcggcaag gcgctcgacc
     6541 tcctggacca agcctacggc aaggccctgc gcgcggcggt cgcccatcgg ccgaccgtcg
     6601 tgatcctcgt cgtcggctgc ttcgccctgt ccctcgcctt tatcccggac atgaacatca
     6661 acatgatgcc gaactccgac gattcgtcca tcaatctgtc ggtgacgatg ccgctcggca
     6721 ccaagctcgg cgagaccaag gccgtgatgc tcgcgatgga ggcctacgcg atggacgagc
     6781 tcgagggcgt cgagaccatc atcacgacgg tcggcggcgg catgatgggc ggcggctcga
     6841 gtagcaacgg cgggcagcta tccgtcacgc tgcccgactt ctccgagcgc gtcgacgacg
     6901 ccgtcacgat gcaggccaag ctacgcgccc atttctccga attcccgaac gccgccttca
     6961 gcttctccat gggacgcatg cgccagatga gcggcgccga catcgacgtg gtcatacgga
     7021 cccaagacct cgaggtgggt atagcgaccg cgcatacgct gatcgacctg ctcgaggaga
     7081 aggtgcccga cctcagggac gtggcgatgg acctgaacga gggcttgccg caggtcgagg
     7141 tgatcatcga ccgggagcgg gcctacgcct tcggcataac cgtgcaggcc atagccaagg
     7201 agatagacgc gagcgtcgac ggggtgaccg cgacgaccta ccgcgaggcg ggcgacgagt
     7261 acgccgtcac cctggtcctt cgcgaggcgg acaggagcaa gctacccgac ctggagaaca
     7321 tctacgtgca gggcggtatg gggcgtgtcg cggtggccaa cttcgcctcg ctcgagaagg
     7381 gcgtcggccc ggtcagcatc tcgagggagg atcagtcccg cgtcatccac gtgaccgcga
     7441 gcatcacgag cgaccgccgc gccggcgacg tcgaggacga catcaaggcg gtcatagcgg
     7501 acaacgtcgt cctgcccgag ggcacgaggc tatcgtacga gggctcctgg gcgagcgtga
     7561 tggagacggg cggatacttc gtcctgatcg tcacgatggc cctgctcctc gtcttcggcg
     7621 tgatggccgg acagtacgag tcgtggaagg acccgatcat caacatgttc acgataccgc
     7681 tgctgctgat cggcgtcgtg gcggtctacg tgatcaccgg cgagagcctg accatgttca
     7741 ccgcgatggg cgtggtgatg ctcatcggca tcgtcgtcaa caacggcatc gtactggtcg
     7801 actacacgaa cctgctcgtg cgccgaggca tgccggtccg cgaggcctgc gtgcgaggcg
     7861 gcgcgtcgag gctcaggccc gtgctcatga ccacgctgac gacggtgctc ggcctcctcc
     7921 cgatggctct atcgaacggt gagaattccc agatgctcaa gccgataggc ctgacggtac
     7981 tcggggggct cctgtcgagt acgctcatca ccctgttctt catacccgtg atgtactcgt
     8041 tcttcaacga gcggcgcgtc cgcgtggcgg ccaaggagtc agcatgacga ggatagagat
     8101 catagccaac cgctcggtcc aggacgacgt cgtcgacggg ctcgagtccg ccatcgacgg
     8161 cttctactat acggtggtgc ccgtggtcca cgggcgcggc cgcgaggcga ggatgctcgg
     8221 caccgccacc tggcccgagg agaatttcat gctgatagcc tatatagacg acggccaggc
     8281 cgaggtcgcg agagccgtcg tcgccaaggt caaggcgcgc ttcccgaccg agggcctcaa
     8341 gctcttcgcc gtcgggggcg cctcgtcgct cggctcgtag tacggtccgg cgcgccggtc
     8401 ggacggttcg ggagccgagc gaggctccgg attcctcctt gagcccggcg ggcgcgtcga
     8461 tcccatacta taaaaacggg ccgccccggc tagccggggc ggcccttctc cttttttagg
     8521 aaccgatcgg gaaactaccc gaggatcaca cgaggccctg ggcgatcatc gcgtcggcta
     8581 ccttcttgaa gccggcgatg ttcgcgccca cgacgtagtt gccgtcggcg ccgaattcct
     8641 tggccgcctc gaggcaggta ttgtggatgt tgaccatgat ggcatgcagc ctctggtcga
     8701 cttcctcggc cgtccaggcg aggcgcatcg agttctggct catctcgagg ccggaggtgg
     8761 cgacgccgcc ggcgttcgct gccttgcccg ggccgaaggg gatcttcttc tcgacgaaga
     8821 gcttccaggc ctcgggggtg cagcccatgt tcgagacctc gacgacgtac ttgacgccat
     8881 tcttgacgag catctgggcg tcgtcgcctg acatctcgtt ctggatggcg cagcacatgg
     8941 cgatgtcgac cttctgctcc cagggcctct tgccggcgaa gaacttggcg ttcgggaact
     9001 tcttggcgta ggtctcgcag gagttgcggt tgatgacgag catttcctcg aggtacttgt
     9061 gcttctcggc ggtggagatg ccgtcgggat cgtagatgta gccgtccggg ccggagatcg
     9121 tgacgacctt agcgccgagc tcgctggcct tgatgcaggt gccccaggag acgttgccga
     9181 agccggagac ggagacggtc ttgcccttga gcgtgtcgcc ctggggcacc tgcatcaagg
     9241 ccagcga
//
LOCUS       DAOPHV010000028         9218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_9384_length_9218_cov_3.900033, whole genome shotgun sequence.
ACCESSION   DAOPHV010000028 DAOPHV010000000
VERSION     DAOPHV010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9218
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9384_length_9218_cov_3.900033"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PLQ29_01450"
     CDS             <1..667
                     /locus_tag="PLQ29_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPG85337.1"
                     /translation="ADDLCDLGRVDVTEDDVALLSGPRGPEAVNVAWVLGSMFGSSSL
                     VARAARRSAGVVRSLNAYLELSPPDESALVDFDIAEKLLGRYCEAGSTGRTVRFELED
                     GVSAYGDPDRLDLVWENLISNAMFAAGIDGNVTVRARFDGAATVVEVEDDGPGVDPAI
                     RDRLFEPYVSAKALEEGKGLGLDICKRIVDTHRGTITFESRPGRTVFTVRLPGEPDGA
                     DSL"
     gene            664..1071
                     /locus_tag="PLQ29_01455"
     CDS             664..1071
                     /locus_tag="PLQ29_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPG85338.1"
                     /translation="MKSEAILCVDDEAIILMAMKRELKTHFRDRFRFETALDAAEGLR
                     IIDALSEDGVSVILVISDWLMPGMKGDEFLLRIRSLHPDIRTIMVTGHADPASIERTI
                     KEAGAFTVLQKPWNRGELIAAISACVGPERSLA"
     gene            1174..2934
                     /gene="argS"
                     /locus_tag="PLQ29_01460"
     CDS             1174..2934
                     /gene="argS"
                     /locus_tag="PLQ29_01460"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPG85339.1"
                     /translation="MRSVREAWKAAVAATVADMLAEKGADPALSEAVAAEAPPDAAMG
                     DVGFPMFPFAKVLRMGPPQIAAALAERLSAKAGQAPAGTVKAVGPYLNVFLDRASVAE
                     QALSSRSLGASYGRSSSLAGSKVMIEFSSPNTNKPLHLGHLRNDVLGESSARILRANG
                     AEVMKINLVNDRGVHICKSMLSYMAYGGGRTPEDEGKKTDHFVGDYYVKFNELKKEDP
                     RAEEKAQELLVKWEAGDPETIALWKKMNEWAVGGIRRTYERTGVSFDKYYYESQIYLT
                     GKDEVQKGLASGVFFKAEDGSVRVDLTPIGLEEKVLLRKDGTSIYMTQDLGLAIHRHA
                     DWPFDRLVYVVANEQQYHFKVLFYILKRLGYEWADSLFHLSYGLVNLPEGRMKTREGT
                     VVDADSLLDSLRELALEEIQAKGREDAVGDPQDTAEKIALGAVHYYLAPATPSKDMIF
                     NPKESLSFNGDTGPYIQYMGARISSLLRKRDRGEGGAAKGEVRPELLSSPTEWELVRL
                     VSSYPEAVEQAGAEYNPAIVAAHMYAVASAFSKFYHDEPILNCDEPDRAATRLALSEA
                     VLVVLKNGLELLCIPFLEAM"
     gene            3113..3424
                     /gene="rplU"
                     /locus_tag="PLQ29_01465"
     CDS             3113..3424
                     /gene="rplU"
                     /locus_tag="PLQ29_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPG85340.1"
                     /translation="MYAIVEVQGKQYRAEKGAVLRVDRMDGEAGTPVAIDSVLLVSGD
                     TIKIGAPYVQGAQVKATIKAQEKGDKVIVFKYKPKKDYRRTQGHRQRYTLLTVEDIVG
                     A"
     gene            3414..3755
                     /locus_tag="PLQ29_01470"
     CDS             3414..3755
                     /locus_tag="PLQ29_01470"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HPG85341.1"
                     /translation="MGPDRLIVRLSFWPDGALKGARADGHAGDRRAGANLACAAVTVL
                     LRTAYETAAGYEGVTVRGIAPAPGSLSFEIGRYPPAMAERLRGVGDFLTVGLSSVDRE
                     YPGLIDFLLER"
     gene            3768..4007
                     /gene="rpmA"
                     /locus_tag="PLQ29_01475"
     CDS             3768..4007
                     /gene="rpmA"
                     /locus_tag="PLQ29_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPG85342.1"
                     /translation="MPHKGNGRDSNPQHLGVKAYGNELVRAGSIIVRQRGSKIHPGEN
                     VGKGKDDTLFAKADGRVVFCEHRGRKYAGIEPIES"
     gene            4132..5163
                     /gene="obgE"
                     /locus_tag="PLQ29_01480"
     CDS             4132..5163
                     /gene="obgE"
                     /locus_tag="PLQ29_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPG85343.1"
                     /translation="MVHFADETRITVSSGNGGNGCVAFRREAHVPLGGPAGGDGGRGG
                     DVVFEVRRNLRTLVHIRYAKHYRAQAGQDGMGRNRHGKDGEDVVIPVPPGTVIKDSGT
                     GETLMDFGTSGEGRWVFLRGGNGGWGNVHFKSSVNQAPRTALPGKPGESVELRVELQL
                     MADIGFVGFPNAGKSSLLDRCTNARPKIAPYPFTTKIPNLGVLTVAERDIILADIPGI
                     IEGAHEGAGLGIRFLKHISRTAALAFLVDLSDDGFLGAFEVLLAELGGFSAELLAKPR
                     IVIGTKLDLPETAGRLDELRAALPDERVVGLSVFSGEGLGAISQAFLSFVLEAERAQA
                     SGGLAGAEP"
     gene            5160..5732
                     /gene="nadD"
                     /locus_tag="PLQ29_01485"
     CDS             5160..5732
                     /gene="nadD"
                     /locus_tag="PLQ29_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HPG85344.1"
                     /translation="MKLLILGGTFNPIHVGHLMLAEEAALEFGYDRVALVPSSIPPHK
                     RPEGDPGPAARLEMTRAAVDGYGAFIVEDCEIERGGTSYTVDTLERIVAAYPLEGRPG
                     LLIGDDLASGFESWRDPDGVCRLADLIVARRLGGLDALERPHRRASNMLLPISSTDIR
                     SRIRSGRPWTWLVPEPTVRYILLNGLYGSA"
     gene            5770..6945
                     /locus_tag="PLQ29_01490"
     CDS             5770..6945
                     /locus_tag="PLQ29_01490"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPG85345.1"
                     /translation="MGRSRIDISIAFLAGILLIAVAGVLIMVSALRVDPVEEAVAEER
                     IINVALIVERSGKPASTQLLMYYPGNARAAMLDIPSETGLIISSLNRVDSIDALYEPD
                     DPRPFLDEVQRLTGATIDWYLILDEGSLSSLVDLLDGISVFLPERISDMVNEVPVSLP
                     SGFVVLDGRKTLVYASYTQADAMDSELVAKRQSLSQAALRRLGERSDYAMRDDVFPVM
                     RSLFRTNLKPESLRRLFEEFVRLEADRIVLQRLTGANRYVEGKLLLFPYWDGELVKDI
                     VKQSLNALLNADATAIADKVFTLEILNGTATRGLAQKTADIFESFGYEVVSVGNAESS
                     DVAKTSVIDRYGDLVSATSVANVIRCKIVEAEAIPSASPADFVIILGKDFNGRNCGD"
     gene            6935..7342
                     /gene="rsfS"
                     /locus_tag="PLQ29_01495"
     CDS             6935..7342
                     /gene="rsfS"
                     /locus_tag="PLQ29_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPG85346.1"
                     /translation="MATEVREAAFGCAAMLAEHNGGDTVVLDLSKLSTWTDFFVVTSA
                     TSSTHLRGLARRVEEYLGPLGFSPLRRPGLADDEQWCLLDFGDFVVHIMTAEARGFYE
                     LEKLWFGAEITALSAPTAPASTAPASAAPARSD"
     gene            complement(7395..7712)
                     /locus_tag="PLQ29_01500"
     CDS             complement(7395..7712)
                     /locus_tag="PLQ29_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85347.1"
                     /translation="MDPIRDTAMPGNALELALMGVGEYAQVPEAPFVRFDDYEDEYED
                     DFEDEDDFDDFSEEEESFDDDEDDDFDDDEDDDFDDDFDEEDGESGDFDYEDDIDYND
                     FDE"
     gene            7907..8812
                     /gene="ispE"
                     /locus_tag="PLQ29_01505"
     CDS             7907..8812
                     /gene="ispE"
                     /locus_tag="PLQ29_01505"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPG85348.1"
                     /translation="MTKPKIVMAPAKINLHLAVGSSRNDGFHTIASIFQAISLCDRVS
                     LAAIDGDETTLACECDCPVEKNTAWRAAMAFRRAAAVRVVIPALSIVVEKGIPAGAGL
                     GGGSSDAAATLIGLYRMFPGAVDRLELLRIAEGIGSDVPFFMESACAAVSGRGERLTP
                     IKPRLDYALVIVDPGFPISTKDAYDRLDQARAAGRVPPATGDAAMDRELEAMVDSYER
                     VQLAAWPFRNDFFEALVPDYPALEACKAELLASGAVFASMSGSGSVVFGIYASSDHAE
                     HVRASFSKRYRAYTAFPLARLHQSI"
     gene            8855..9133
                     /gene="spoVG"
                     /locus_tag="PLQ29_01510"
     CDS             8855..9133
                     /gene="spoVG"
                     /locus_tag="PLQ29_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HPG85349.1"
                     /translation="MDITDIRIRRVSGEGKLRAYVTVTFDNCFVIHNVKLIDGRAGIF
                     MAMPSRKTKNGDYKDVAHPICPSFRSMMEEKILALYTAGAGVEDDGQD"
BASE COUNT         1658 a         2915 c         3001 g         1644 t
ORIGIN      
        1 ggccgacgac ctgtgcgacc tggggcgggt cgacgtaact gaagacgacg tggcgctcct
       61 gtccgggccc cgcggccccg aggctgtcaa cgtcgcctgg gtgctcggat cgatgttcgg
      121 ctcatcgtcg ctcgtcgcga gggcggcgag gcgttcagcc ggagtcgtac gatcgctgaa
      181 cgcctatctg gagctgtccc cgccggacga gtcggccctc gtcgatttcg atatagcgga
      241 gaagcttctc ggccgctact gcgaggccgg gtccacgggc cgaaccgttc gcttcgagct
      301 cgaggatggc gtgagcgcct acggcgaccc cgatcggctc gacctggttt gggagaatct
      361 catatcgaac gcgatgttcg cggccgggat agacgggaat gtcacggtca gggcgcgttt
      421 cgacggagcg gcgaccgtcg tggaggtgga ggacgacggc ccgggcgtcg atccggccat
      481 ccgcgatcgg ctgttcgagc catacgtatc ggccaaggcg ctcgaggagg gcaagggctt
      541 gggcttggac atatgcaagc gaatagtgga tactcatagg ggtacgatca cattcgagag
      601 ccgtcctgga cgtaccgtct tcacggtgcg cctgccaggc gaacccgacg gagcggacag
      661 tctatgaaga gcgaagcgat cctgtgcgtc gacgacgagg ccatcatcct gatggccatg
      721 aagcgcgagc tcaagacgca ttttagggat cggttccggt tcgagaccgc tttggacgcg
      781 gccgaagggc tcaggatcat cgatgccctg tccgaggacg gggtctcggt catactcgtc
      841 atttccgatt ggctcatgcc cggcatgaag ggcgacgaat tcctcctgag gatacgatcc
      901 ctccatcccg atatacggac catcatggtc actggccacg ccgatcccgc ctccatcgag
      961 cggacgatca aggaggccgg ggcctttacg gtgctccaga agccctggaa caggggcgag
     1021 ctcatagcgg cgatctccgc ctgcgtcggg ccggagcgaa gcttggccta gccccggctc
     1081 gactcgttga aacgagccgc cctattcgtt acactggcgt ccataccatg gcggcccggc
     1141 ctcgagtcgg gtatcgccca gcaggagcca gccgtgagat ccgtacgaga agcgtggaaa
     1201 gccgcggtgg cggcgaccgt agccgatatg ctcgcagaga aaggcgccga cccggccctc
     1261 tccgaggccg tcgcggccga ggcgccgccc gacgccgcca tgggcgacgt cggctttccg
     1321 atgttcccgt tcgccaaggt cctccgcatg gggcctcccc agatcgccgc ggcgctggcc
     1381 gagcgcctgt cggccaaggc cggccaggcg cctgccggca ccgtcaaggc cgtcgggccg
     1441 tacttgaacg tgttcctgga ccgggcctcc gtcgctgaac aggcgctctc ctcgcggtcg
     1501 ctcggcgcgt cgtacggccg ctcgtcgtct ctggccggct ccaaggtaat gatagagttc
     1561 tccagcccga acactaacaa gcccctgcac ctcggccacc tgcggaacga cgtgcttggc
     1621 gagagctcgg cgcgcatcct cagggccaac ggcgccgagg tgatgaagat caatctggtc
     1681 aacgaccgcg gcgtgcatat ctgcaagtcg atgctgtcgt acatggccta cggcggcggc
     1741 cggacccccg aggacgaggg caagaagacc gaccacttcg tcggtgacta ctacgtcaag
     1801 ttcaacgagc tgaagaagga agaccccagg gccgaggaga aggcccagga actgctcgtc
     1861 aagtgggaag ccggcgaccc cgagacgatc gcgctctgga agaagatgaa cgagtgggcc
     1921 gtcggcggca tcaggcggac ctacgagcgg accggcgtat ccttcgacaa gtactactac
     1981 gagagccaaa tctacctgac cggtaaggac gaggttcaga aggggctggc ctccggcgtc
     2041 ttcttcaaag ccgaggacgg ttcggtccgc gtcgacctga cgccgatcgg gctcgaggag
     2101 aaagtgctgc tacgcaagga cggtacctcc atctacatga cccaggacct gggcttggcc
     2161 atccaccggc atgcggactg gcccttcgac cgactggtct acgtcgtcgc caacgagcag
     2221 cagtaccatt tcaaggtgct gttctacata ctgaagcgcc tcggctacga gtgggcggat
     2281 agcctgttcc acctgtcgta tggcctcgtc aacctgcccg agggccggat gaagacccgc
     2341 gagggcaccg tcgtcgatgc cgactcgctg ctcgactcgc tcagggagct ggccctcgag
     2401 gagatccagg ccaagggccg cgaggacgcc gtcggcgacc cccaggatac ggccgagaag
     2461 atagcgctcg gcgccgtgca ctactacctg gccccggcca cgccttccaa ggacatgatc
     2521 ttcaacccca aggagtcgct gtcgttcaac ggcgacacgg gcccctatat ccagtacatg
     2581 ggcgccagga tctcgagcct cctgcgtaag cgcgaccggg gcgagggcgg cgccgcgaag
     2641 ggagaggtac gtccggagct actgtcgtcg ccgaccgagt gggagctcgt caggctcgtc
     2701 tcctcctatc cggaggccgt cgagcaggcc ggcgccgagt acaacccggc tatcgtcgcc
     2761 gcccatatgt acgcggtcgc gagcgccttc tccaaattct accacgacga gcctatcctg
     2821 aactgcgacg agccggaccg ggccgcgacg cgtctggccc tgtcggaggc ggtgctcgtc
     2881 gtgctcaaga acgggctcga gctcctgtgc ataccgttcc tggaggcgat gtagcgagcc
     2941 gtcgccgctt cggttgacat tggcgggagt tgcgtagtat aaaatcgagc cggtacgccg
     3001 ttccgaggcg ccggttcttg gtcgggctcg gccatgatca ttgtacgcgt cggccgatac
     3061 cggatggcaa cgtccctcgg cctcggcata cgaaacataa gaggttgaca ggatgtacgc
     3121 aatcgtagaa gtgcaaggca agcagtaccg cgccgagaag ggcgccgttc tccgcgtgga
     3181 ccgcatggac ggcgaggccg gaaccccggt cgccatcgat tctgttctgc tcgtgtccgg
     3241 tgatacgatc aagatcggcg ctccctacgt tcagggcgcc caggtgaagg cgacgatcaa
     3301 ggcccaggag aagggcgaca aggtcatcgt gttcaagtac aagcccaaga aggactaccg
     3361 ccggacccag ggccataggc agcgctacac gctccttacg gtcgaggata tcgttggggc
     3421 ctgataggct tatcgttagg ctatcgttct ggccggacgg ggcgctcaag ggcgcccgcg
     3481 ccgacggaca cgccggcgac cgccgggcgg gcgccaacct ggcttgcgcc gcagtcaccg
     3541 tcctcctgag gacggcgtac gagacggccg ccggctacga aggcgttacg gtcaggggca
     3601 tagccccggc gccgggtagc ctgagcttcg agattgggcg gtacccgccc gcgatggccg
     3661 agaggctgcg cggcgtcggt gacttcctga ccgtgggatt gtccagcgtg gatcgcgagt
     3721 atccgggctt gatagatttt ctattagaga gatgacggag gttctatatg ccacataagg
     3781 gtaacggccg cgattcgaat ccgcagcatc tgggcgtcaa ggcgtacggc aacgagctcg
     3841 tgcgcgcagg ctcgatcatc gtgcgccagc gcggctccaa gatccatccc ggcgagaacg
     3901 tcggcaaggg caaggacgac acgctgttcg ccaaggccga cggccgggtt gtattctgcg
     3961 agcaccgcgg ccgcaagtac gccggcatcg agccgataga gtcctaaagg cctcgccgcg
     4021 gcgacgcggc cagcatcgag cgcgactttc ggatagtcgc gcctcacgcc gtcgcgtccg
     4081 gccatcggcc gagcggcggc ttttctatct ccggaaggga cgggtagcgc gatggtacat
     4141 ttcgccgacg agacgaggat caccgtatct tccggcaacg ggggcaacgg ttgcgtggcg
     4201 ttcaggcgcg aggcccacgt gccgctcgga ggcccggctg gtggcgacgg tggccgcggc
     4261 ggcgatgtcg tcttcgaggt gcgacgcaac ctgaggacgc tcgtgcatat ccgctatgcc
     4321 aagcattacc gtgcccaggc gggtcaggac ggcatgggtc ggaatcgcca tggcaaggac
     4381 ggagaggacg tcgtcatacc ggtacccccg ggcacggtca tcaaggactc cgggaccggc
     4441 gaaaccctca tggacttcgg cacttccggc gagggacggt gggtcttcct gcgcggcggc
     4501 aatggcggct ggggcaacgt gcacttcaag tcgagcgtca accaggcgcc gcgcacggcc
     4561 ctgccgggca agccgggcga gagcgtcgag ctacgcgtcg agctacagct gatggccgac
     4621 attggcttcg tcggcttccc gaacgcgggg aagtcgagcc tgctcgaccg ctgtacgaac
     4681 gcgcggccga agatagcgcc gtaccccttc acgaccaaga taccgaacct cggcgtgctg
     4741 accgtggccg agcgcgacat cattctggcc gatatacccg gcatcatcga gggcgcccac
     4801 gagggggccg gcctgggcat acgcttcctt aagcatatct cgcgcaccgc ggcgctggct
     4861 ttcctcgtcg acctttcgga cgacggcttc ctgggtgcct tcgaggtgct gctcgccgag
     4921 ctcgggggct tctccgccga gctgctagcg aagccgcgca tcgttatagg caccaagctc
     4981 gacctgccgg agacggccgg ccgcctcgac gagctccgcg cggccttgcc ggacgagcgc
     5041 gtcgtcgggc tctccgtctt ctcgggcgag gggctcggcg ccatctcgca ggcgttcctg
     5101 tcgttcgtac tggaggccga gcgagcccag gcgtccgggg gcctggccgg cgccgaacca
     5161 tgaagctcct gatcctcggc ggcaccttca accccatcca tgtcggtcac ctgatgctgg
     5221 ccgaggaggc cgcgctggaa tttggctacg accgggtggc gctcgtgccc tcgagcatac
     5281 cgccgcataa gaggccggag ggcgatcccg gacccgcggc gcggctcgag atgacccggg
     5341 cggccgtcga cggatacggc gcgttcatcg tggaggactg cgagatcgag cgcggcggca
     5401 cgtcgtatac ggtcgatacg ctcgagcgta tcgtcgcggc gtacccattg gagggacgcc
     5461 cgggcttgct gatcggcgac gacctggcgt ccgggttcga gtcgtggcgg gaccccgacg
     5521 gcgtttgccg cctggctgac ctgatcgtgg ccaggcgcct cggcgggctg gacgccctgg
     5581 aacgccccca taggcgagcc tccaatatgc tactcccaat ctcgtcgacg gatattcgct
     5641 ccaggatacg ttctggacgg ccctggacct ggctggtacc tgagccgacc gtacggtata
     5701 ttctgctgaa cggcttatac ggtagcgcgt agcggtcccg gccgtcgcga gctgaaagag
     5761 taaggtagca tgggtcgatc acggatagac ataagcatcg cgttcctcgc gggtattctc
     5821 ctcatcgcgg tggcgggtgt cctgatcatg gtgtcggcgc tacgcgtcga cccggtcgag
     5881 gaggcggtgg ccgaggagcg gatcatcaac gtcgccctca tcgtcgaacg gtccggtaaa
     5941 cccgcgtcga cccagctgct gatgtactac cccggcaacg cgagggcggc catgctcgac
     6001 ataccgtcgg agaccggcct gatcatctcg tcgctcaacc gcgtcgacag catagacgcg
     6061 ctgtacgagc ccgacgatcc gcggcccttc ctcgacgagg tgcagcgcct gaccggcgcg
     6121 acgatagact ggtacctgat actcgacgaa gggtcgctgt cgtcgctcgt cgatctgctc
     6181 gacggcatct cggtgttcct gcccgagcgg atatccgaca tggtcaacga ggtgccggta
     6241 tcgctgccgt ctggcttcgt cgtgctggat ggacgcaaga ctctcgtcta cgcctcgtat
     6301 acccaggcgg acgcgatgga ctcggagctg gtcgccaaac gccagtcact cagccaggcc
     6361 gcactcaggc ggctaggcga gcggtccgac tacgcgatgc gcgacgacgt gtttccggtc
     6421 atgaggtcgc tgtttaggac aaatctgaag cctgagtcgc tcaggcgcct gttcgaggaa
     6481 ttcgtccgac tcgaagccga caggatagtg ctgcaacgcc tgaccggcgc caatcgctat
     6541 gtcgagggca agttgctcct gttcccgtac tgggacggcg agctcgtcaa ggacatagtc
     6601 aagcagtcgc tcaacgcact gctcaacgcg gacgctaccg ccattgccga caaggtcttt
     6661 acgctcgaga tcctgaacgg caccgcgacg agaggactcg cccagaagac tgccgacata
     6721 ttcgagagct tcggctacga ggtcgtgtcc gtcgggaacg ccgagtcgag cgacgtagcg
     6781 aagaccagcg tgatcgaccg ctacggagac ctcgtctcgg ccacgagcgt ggccaacgtg
     6841 atccgctgca agatcgtcga ggcggaggcg attccgagcg ccagtccagc cgacttcgtc
     6901 ataatactcg gaaaggattt caatgggagg aactgtggcg actgaggtac gagaggcggc
     6961 cttcggctgc gcggccatgc tagccgagca taacgggggc gatacggtcg tcctggacct
     7021 gagcaagctg tcgacctgga ctgatttctt cgtggtaacg agcgcgacca gttcgacgca
     7081 cctgaggggc ctcgcgcgcc gcgtcgagga gtacctcggg ccgctcggct tctcgccgct
     7141 caggcggcct ggcctggccg atgacgagca gtggtgcctg ctcgatttcg gggacttcgt
     7201 ggtgcatatc atgaccgccg aggccagagg cttctacgag ctcgagaaac tgtggttcgg
     7261 agccgagatc acggccctca gcgccccgac ggctcccgcg tccacggctc ccgcgtccgc
     7321 ggctcccgcc cgctcggatt aaaaaaggcc gcgcctccgg tccgggggcg cggcctttcg
     7381 tcgtcttgcc gaggctattc gtcgaaatcg ttatagtcga tatcgtcctc gtagtcgaaa
     7441 tcccctgatt ctccgtcttc ttcgtcgaag tcgtcgtcga agtcgtcatc ctcgtcgtcg
     7501 tcgaagtcgt catcctcgtc gtcgtcgaag ctctcttctt cttcggaaaa gtcgtcgaag
     7561 tcgtcctcgt cttcgaagtc gtcctcgtac tcatcttcgt agtcgtcgaa gcggacgaaa
     7621 ggagcctcgg gaacctgggc gtactcgccg acgcccatga gcgctagctc caacgcgttt
     7681 cccggcatcg ccgtatcccg tataggatcc atttcaatcc tccccataaa gtatcgtcgt
     7741 atgagccctc ttgtggcttc cccgctcccg cacggcctta aaccggacta gtgggtacgg
     7801 agaaatctaa aagagaccag agcggtctgt caactataat cgcgatggaa acatgcgttt
     7861 tattctgcgc ttggcgtagg cggccgtcga tcgctatagt atgcccatga ccaagccaaa
     7921 gatagtgatg gcgcccgcta agatcaacct ccatcttgct gtcggatcgt ccaggaacga
     7981 tggtttccat acaatcgcta gcatcttcca ggccatctcc ctctgtgacc gggtgagcct
     8041 cgcggccatc gacggggacg agacgaccct cgcctgcgag tgcgactgcc cggtagagaa
     8101 gaatacggcc tggcgcgccg cgatggcctt ccgccgggcg gcggcggtac gggtcgttat
     8161 cccggccctg tccattgtcg tagagaaggg tattccggcc ggggcgggac tcggcggcgg
     8221 ctcgagcgac gccgcggcga cgttgatcgg gctctaccgg atgtttcccg gcgccgtgga
     8281 ccggctcgag ttgcttcgta tcgccgaggg tatcggctcc gatgtgccct ttttcatgga
     8341 aagcgcctgc gccgcggtat cgggccgggg cgagaggctg acgccgatta agccgaggct
     8401 cgattacgcc ctggtgatcg tcgacccagg gttcccgatc tcgacgaaag acgcgtacga
     8461 tcgcctcgac caggccaggg cggcgggccg cgtaccgccg gccaccggcg atgcggccat
     8521 ggacagggaa ctcgaggcca tggtcgactc gtacgagcgc gtccagcttg ccgcttggcc
     8581 gttccggaac gatttcttcg aggcgctcgt acccgactat cctgccctcg aggcctgcaa
     8641 ggccgaactc ttggccagcg gagccgtatt cgcctcgatg agcggctccg gtagtgtcgt
     8701 cttcggcatc tacgcctctt cagaccatgc cgaacatgtt cgcgcatcct tttcgaaacg
     8761 ctatcgcgcg tacacggcct ttccgcttgc gcggcttcat cagtcgatat agaattactg
     8821 cgaagtagcg caaacggtaa ggagagagcg ccgcatggac atcaccgata tccgcatccg
     8881 tagggtctcc ggcgaaggaa agctcagggc gtacgttacg gttaccttcg ataactgctt
     8941 tgttatccat aatgtcaagc tcatagacgg ccgtgcgggt atcttcatgg caatgccgag
     9001 ccgtaagacc aagaacggcg attataagga cgtcgcgcat cccatctgcc cgagcttcag
     9061 gagcatgatg gaggagaaga tcctggccct ctacacggcc ggcgccggcg tcgaagacga
     9121 cgggcaggac tagcgaccgt cggagccgcg atacccccgt gacacgataa cgccttttag
     9181 ggtataacta ctggccgtac gtcccttgcg cggcggcg
//
LOCUS       DAOPHV010000029         9110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_9526_length_9110_cov_3.701712, whole genome shotgun sequence.
ACCESSION   DAOPHV010000029 DAOPHV010000000
VERSION     DAOPHV010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9110
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9526_length_9110_cov_3.701712"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLQ29_01515"
     CDS             <1..363
                     /locus_tag="PLQ29_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HPG85350.1"
                     /translation="RWDNPQYITDVRNRELRIDPLYPRSTPFVKFAGFLISRDAAAIG
                     GDFIAYVKDVKILYDKAVLEPVRDIDDEAIWGIIGQKEDDRKRLESQRFGASQVMRYL
                     ERMKQETKTEFTGSGTSE"
     gene            459..1703
                     /locus_tag="PLQ29_01520"
     CDS             459..1703
                     /locus_tag="PLQ29_01520"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG85351.1"
                     /translation="MDASEPIQSRASLERAYKSNAKDIGAILNEMVARIQGALDAVGL
                     FPAYKHRVKSFQSYFAKKIKFTRDAVTNGAPPLPITDLIAVRIICPFIGDLERVEKAL
                     SERFVVREVERKGSERSFKEFGYESTHLLLEIPEDVLAGNTFIDPSVCEVQIRTMLQD
                     AWAEVEHELVYKAEFTPFDDPMKRKLAALNANLTLSDMLFQEIRDYQHQLTNELAKRR
                     VAFFKKIEDAIDKDLYTNFKETPRSASEEPKQPSWLGPWKSLDDLLLEALSAHNRADY
                     IRAIDIYSHILALNPKPEIRALIYKHRGMAFFAESRYDEALEDFTATLELDGKCYKAA
                     YYRGVVHSVKQNYSAAIDDFNDAVAIHPYHFYSTYRRAQAYYHVEDYPKALADCDAAL
                     ELEPENESLKKLKRMVLKKLKM"
     gene            complement(1732..1803)
                     /locus_tag="PLQ29_01525"
     tRNA            complement(1732..1803)
                     /locus_tag="PLQ29_01525"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1768..1770),aa:Glu,seq:ttc)
     gene            1954..2424
                     /locus_tag="PLQ29_01530"
     CDS             1954..2424
                     /locus_tag="PLQ29_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85352.1"
                     /translation="MKLKVTVIALVLLIAVAVPMTFATGVGAAFGLPFGGGLPGSNVM
                     LSLKVSQIPFLMGLGFNISETNFAFGLTGDWWVANANLFSFINYYLGPGFYLGFGNHD
                     FLLGGRFPVGLNVFPIKNLELFLEIAPTLAVGIGDSISFPNWGFQSAFGLRFWF"
     gene            2533..2901
                     /gene="trxA"
                     /locus_tag="PLQ29_01535"
     CDS             2533..2901
                     /gene="trxA"
                     /locus_tag="PLQ29_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPG85353.1"
                     /translation="MTEHLTLAMFKERVFDYENEKEWKYRGELPAIIDFYADWCGPCK
                     MVAPLLEKLSGKYEGKLVIYKVDTEAEQELASMFGIQSIPTILFVPTVGQPRVSMGAI
                     PEKEFERLISELLLPVEAQG"
     gene            complement(2864..4060)
                     /gene="thiI"
                     /locus_tag="PLQ29_01540"
     CDS             complement(2864..4060)
                     /gene="thiI"
                     /locus_tag="PLQ29_01540"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HPG85354.1"
                     /translation="MDALYLIKIGEIRLKEGNRTEFEQRLKHDLKRRLSSIHSEVRHR
                     DGRYYLTVAEEHAGLAEFVLARTPGVNGWARAVMTSKDIDAVRAAVLEAAAAVVARGA
                     ATFKVEARRSDKSFPLSSYEIASGLGADILERFPSLRVDVRAPEATLYVEIREKAYVY
                     TDGYPGVRGLPIGSGGRGLLLLSGGIDSPVAGYRMLCRGLSLESLYFHAYPYTSREAW
                     EKVRDLAATLASFSGGMTLHTASFTDVQLRIKKDAPAEKTTLYLRACMMIAADMVAKA
                     RGLNAIVSGESLGQVASQTAENMRFTGSFTDHAILRPLVGTDKEDTIRTARAIGSYEL
                     SILPYEDCCVLFSPKHPILKADFAAEREAFEALGLREAVAESVATMETLEVPFSPELP
                     RAGAAR"
     gene            complement(4105..5247)
                     /locus_tag="PLQ29_01545"
     CDS             complement(4105..5247)
                     /locus_tag="PLQ29_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPG85355.1"
                     /translation="MHYLDWAATAPPYRDILEAAVGVATERYGNPSSRHGAGRDARAL
                     LESARERLGAAIGAAPSSVVFTSGGSEADAIALLSTLVGKGERSVVISELEHPAVYEQ
                     ARALESLGVRVVRVRPERDGVVSPGRVAEALRPDTAVVAVMAVSNETGAIQPIGRIVE
                     AVRSAAGPRRPFVHCDAVQALGTIGFDARALGVDGAAFSAHKLGGPRGVGALYLSRPI
                     SPLVLGGGQEGGMRPGTHNTAGAWAFAEAAARAAASVEARMERARALERRLFDGLRAI
                     PGATPLPEGRLPGDERYSPYIACLSFPGLAGETMTRLLDEAGVAISTGSACSSSKKER
                     RSLDAMGVDRELSFSSIRVSSGRDTSEADIDAFLEAASSAYIRFRV"
     gene            complement(5308..5634)
                     /locus_tag="PLQ29_01550"
     CDS             complement(5308..5634)
                     /locus_tag="PLQ29_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HPG85356.1"
                     /translation="MAAKSIDDTKRFATMVAAKQGWELNPDADFVGSLIEGLTVNWNR
                     YGYFLCPCRDSDGSREADASVICPCAYARDDVAERGHCFCALYLRAGYAAAGGVPGGI
                     PDRRFS"
     gene            complement(5637..5936)
                     /locus_tag="PLQ29_01555"
     CDS             complement(5637..5936)
                     /locus_tag="PLQ29_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HPG85357.1"
                     /translation="MLETLSYIAVDGPRKVPVITVYALSTCGFCKRAMTFLQTNGFAY
                     RYIHMDAIPIEVKNEAKRALKDIYKADVAFPFATVGAKDYLVGFIEADWKRTLGV"
     gene            6028..7848
                     /gene="lepA"
                     /locus_tag="PLQ29_01560"
     CDS             6028..7848
                     /gene="lepA"
                     /locus_tag="PLQ29_01560"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPG85358.1"
                     /translation="MHDIARTRNFCIIAHIDHGKSTLADRFIERAKLVDQRKMQQQML
                     DNMDIERERGITIKSQAVTLSYVARDGLEYTLNLVDTPGHVDFSYEVSRAISSCEGAI
                     LLVDATQGVEAQTLSNMYMAVEHDLEILPVINKIDMPAADVPEARRQIDHDLGLDGDE
                     ALCVSAKLGTGLDELFEAIVARVPAPSGSKDAPLRALIFDSHYDPYRGVIVHMRVFDG
                     SIVPGMAIRLMYNDAEYEVEETGLFKMGLVKTDGLVAGDVGYFIAGIKTVSDVRVGDT
                     VTGAADPVAEPLPGFREVKPVVYSSIYPMDTADYDELKVAMEKLKLNDASLIYEKDNS
                     AALGFGFRCGFLGMLHLEVVQERLEREYDQAIIMTAPSVRYRLTMSHGGDTVIIDNPA
                     DYPDPSRIDGAEEPYIKAQIITPLAYIGNLITLCINKRGSQTGFTHLDEKRVELTFEM
                     PLAEVLFDFYDKLKSTSRGYASFDYEIIDYRPTELAKLDILINSKPVDALAQLVYRPS
                     AMDRGRKVCERLKDSMDRQQFKIAIQAAIGGSIIARETVNPVRKDVLAKCYGGDISRK
                     RKLLEKQKEGKKRMKMVGDVELTQESFMAVLRENEDDDRR"
     gene            complement(7952..>9110)
                     /locus_tag="PLQ29_01565"
     CDS             complement(7952..>9110)
                     /locus_tag="PLQ29_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HPG85359.1"
                     /translation="RMGRLWTRNVVANLGAVDWPSVGPLRGDGRPIVVCGAGPSLDAA
                     IPALRARRDELFVLACDTASGALALSGVTPDAVVCLEGQIYNLEDFLPLGGRRPRFVA
                     DLSAHPSTFRAATGGTSLVLSEYAETRFLSRLSLAGLPLTAVPPLGSVGVLAVRLASA
                     LGGPVLVAGLDFAFEPGRTHCAGSPADLRSRRLESRLCKAESAWNASFRDGVRPIGAG
                     SGEWLTDPALSMYAELAASELRGLEAYDLRCGAGAPLPIRAPLADVAGSAEAVIDAAV
                     RSARDSASAGRRAGTISVAAGPGACRLRAAAFLRGELELAETVAAALRGGSDEQTLRG
                     LILEADFMYCHFPDPERVTALEDDALRRVAAEAAYWRGRLEAAIETADGRR"
BASE COUNT         1663 a         3051 c         2903 g         1493 t
ORIGIN      
        1 cgctgggaca atccccagta catcaccgac gtacgcaacc gcgagctcag gatcgacccg
       61 ctctatccgc gctcgacccc gttcgtcaag ttcgccggct tcctgatctc cagggacgcg
      121 gccgcgatcg gcggcgactt catcgcctac gtcaaggacg taaagatcct gtacgacaag
      181 gccgtcctcg agcccgtccg cgacatcgac gacgaggcca tctggggcat catcggccag
      241 aaggaagacg atcgcaagcg gctcgagagc cagcgcttcg gcgcctccca ggtcatgcgc
      301 tatctcgagc gcatgaagca ggagacgaag accgagttca ccggttcggg caccagcgaa
      361 taacctcagc gcgcgcaagc atatacggcc acccgcgaca gcgggtggct attttttact
      421 cgcttgcccg tccagccata tcgtcgtatc attgacggat ggatgcatcc gaacccatac
      481 agagccgggc gtcgctcgag cgagcgtata agtccaacgc gaaggatatc ggcgctatcc
      541 tgaacgagat ggtcgccagg atacagggcg ccctcgacgc cgtaggcttg ttccccgcct
      601 acaagcacag ggtcaagtcg ttccagagct acttcgccaa gaagatcaag ttcacgcgcg
      661 acgccgtaac gaacggcgcg cccccgcttc cgataacgga cctgatagcc gtccgcatca
      721 tctgcccgtt catcggcgac ctcgagcgcg tagagaaggc gctatcggag cgcttcgtcg
      781 tgcgcgaggt cgagcgcaag ggctcggagc ggtccttcaa ggaattcggc tacgagtcga
      841 cccacctgct gctcgagata cccgaggacg tgctcgcagg caatacgttc atcgacccgt
      901 cggtctgcga ggtgcagatc cgcacgatgc tccaggacgc ctgggccgag gtcgagcacg
      961 agctggtcta caaggccgag ttcacgccgt tcgacgatcc gatgaagcgc aagctggccg
     1021 cgctcaacgc caacctgacc ctgtcggaca tgctgttcca ggagatacgc gactatcagc
     1081 accagctgac caatgagctg gccaagcgcc gcgtggcgtt cttcaagaag atcgaggacg
     1141 ctatcgacaa ggacctgtat acaaacttca aggagacgcc gaggagcgcc tccgaggagc
     1201 ccaagcagcc gtcctggctc ggcccatgga aatcgctcga cgacctcctg ctcgaggcgc
     1261 tcagcgcgca taaccgggcc gactacatac gcgccatcga catctactcg catatactgg
     1321 cgctgaaccc caagccggag atacgcgccc tgatctacaa gcaccggggc atggccttct
     1381 tcgccgagag ccgctacgac gaggccctcg aggacttcac cgccaccctc gagctcgacg
     1441 gcaagtgcta taaggcggcc tactaccggg gcgtcgtcca cagcgtcaag cagaactact
     1501 cggcggccat cgacgatttc aacgacgcgg tcgcgataca cccgtaccat ttctactcga
     1561 cctataggcg ggcccaggcc tactaccacg tcgaggacta cccgaaggcc ctggcagact
     1621 gcgacgcggc gctggagctg gagccggaga acgagtcgct gaagaagctg aagcgtatgg
     1681 tgctcaagaa gctgaagatg taaaaaccgc ccgcgatagc gggcggccgt acatcccctg
     1741 ggagaattga actcccgttg ccgggatgaa aacccggtgt cctaaccact agacgaaggg
     1801 gacgtgctcg tcggagcgac ggtaccttag caggaaatcc gcgcccgtgt caatcgacgg
     1861 cttttcccga cggccggccg cggcccttcg atccgccttg aaatagcgtt atcgctacgg
     1921 tacgattatc catcatatat tcaaggagga tctatgaagc tgaaagtaac cgtcatcgcg
     1981 ctcgtgttgc tgatcgcggt ggcggtgccg atgaccttcg ccaccggcgt cggcgcggct
     2041 ttcggactgc cgttcggcgg cgggctcccg ggctccaacg tgatgctgtc gctcaaggtg
     2101 tcccagatac cgttcttgat ggggctcggc ttcaacatca gcgagacgaa cttcgccttc
     2161 gggttgactg gcgactggtg ggtcgcgaac gccaatctct tctcgttcat caactactac
     2221 ctcgggccgg gcttctacct cggcttcggc aaccacgact tcctgctcgg tggccgtttc
     2281 ccggtgggcc tgaacgtctt cccgatcaag aacctcgaac tgttcctcga gatcgccccg
     2341 accctcgccg tcggtattgg cgacagcatc tccttcccga actggggttt ccagagcgcc
     2401 ttcggcctcc gtttctggtt ctaatagact cggtcacggc caagccgcct gtaatgggcg
     2461 gctttctttt tagagatata gacgaaatat atgaaaaact atatattaag caggcatcaa
     2521 gaaggaggga ccatgaccga gcacctgacc ctggcgatgt ttaaggaacg cgtcttcgac
     2581 tacgagaacg agaaggaatg gaagtatcgt ggggagctac cggctatcat agacttttat
     2641 gccgactggt gcggcccctg caagatggtc gcgccgctac tcgagaagct atccggcaag
     2701 tacgagggca agcttgtgat ctacaaggtc gacacggagg ccgagcagga gctggcgtcg
     2761 atgttcggta tccagagcat cccgaccatc ctgttcgtcc cgacggtggg ccagccccgc
     2821 gtgtcgatgg gcgctatccc ggagaaggag ttcgagcgcc tcatcagcga gctgctcctg
     2881 cccgtggaag ctcagggctg aacggaactt caagcgtctc catcgtagct accgactcgg
     2941 ccaccgcttc acgcaggccg agtgcctcga aggcctcgcg ctcggcggcg aaatcagcct
     3001 tgaggatggg atgtttcggc gagaacagca cgcaacagtc ctcgtagggc aggatggata
     3061 gctcgtagct gcctatagcc ctggccgtcc gtatcgtgtc ctccttgtca gtgccgacga
     3121 ggggtctgag tatcgcgtga tcggtaaacg agccggtaaa gcgcatattc tcggccgtct
     3181 ggctcgccac ctgccccagg ctctctcccg agacgatggc gttgaggccc ctggccttcg
     3241 ccaccatgtc agcggcgatc atcatgcacg cgcggaggta cagcgtggtc ttctccgcgg
     3301 gcgcgtcctt cttgatgcgt agctgcacgt cggtgaatga cgcggtgtgt agcgtcatac
     3361 cgcccgagaa ggaggcaagc gtggccgcca ggtcgcgcac cttctcccac gcctcgcggg
     3421 aggtgtaagg gtaggcgtgg aagtagagcg actcgagcga gagtcctcgg cacagcatcc
     3481 ggtatccggc gactggcgag tcgatgccgc cagacagcag cagcaggccc cggccgccgg
     3541 acccgatcgg caggcctcgg acgcctggat agccgtccgt gtagacgtac gccttctcac
     3601 gtatctccac gtacagggtc gcctcgggag ctcgcacgtc gacccgtagc gacgggaagc
     3661 gttcgaggat gtccgcgcca agcccgctcg ctatctcata ggagctgagc gggaagctct
     3721 tgtcagagcg cctcgcctcg accttgaagg tagcggcgcc gcgcgcgacg acggcggccg
     3781 ccgcctcgag gacggcggcc cggactgcgt caatatcctt actcgtcatg accgcccgag
     3841 cccagccgtt gacgccgggc gtccgcgcca agacgaattc agcgaggccg gcgtgctcct
     3901 cggccacggt caggtagtag cgcccgtcgc ggtgccgcac ctcgctatgt attgacgaca
     3961 agcgtcgctt gaggtcgtgc ttgagccgct gctcgaattc agtacggttg ccttccttga
     4021 gccgtatctc gcctatcttg atcaggtata gcgcgtccat cgcttcgata ctcctatggt
     4081 cgataacttc aggctcgcgc gctcttagac gcggaagcgg atataggccg acgaggcggc
     4141 ctccaggaag gcgtctatgt cggcttcgct tgtatccctg ccggacgaca cgcgtatcga
     4201 cgagaaggac agctctcggt cgacgcccat cgcgtcgagg ctcctgcgct ccttcttgga
     4261 cgacgaacag gccgagccgg tcgatatggc gacgccggcc tcgtcgagga ggcgcgtcat
     4321 cgtctcgccg gctagaccgg ggaacgagag gcaggctatg taggggctgt agcgctcatc
     4381 gccgggcagc cgcccctcgg gtagcggcgt cgcgcccggt atggcgcgta ggccgtcgaa
     4441 caggcgccgc tcgagggcgc gggctcgttc catgcgcgcc tcgacggacg cggcggcgcg
     4501 ggcggccgcc tcggcgaagg cccaggcgcc ggcggtatta tgcgtaccgg gcctcatgcc
     4561 gccttcctgg ccgcccccga ggaccagcgg actgataggc cggctcaggt acagcgctcc
     4621 gacgccgcgc gggccgccga gcttgtgagc gctgaaggcc gccccgtcga ctccgagcgc
     4681 gcgagcgtcg aagccgatgg tgccgagggc ttgcaccgcg tcgcaatgga cgaagggccg
     4741 cctcgggccg gcggccgaac ggacagcctc gacgatgcgc ccgatcggct ggatagcgcc
     4801 ggtctcgttg ctcacggcca tcaccgccac gacggccgta tcgggccgca gagcctcggc
     4861 cacccgcccg gggctcacga cgccgtcgcg ctcgggccgc acgcgtacca ccctgacgcc
     4921 gaggctctcg agggcgcgcg cctgctcgta gaccgccgga tgctcgagtt cggagatcac
     4981 cacggagcgc tcgcccttgc cgacgagcgt cgataggagc gctatggcgt cggcctcgct
     5041 accgcccgag gtgaacacga cgctcgaagg cgccgcacct atagccgccc ccaggcgttc
     5101 cctggccgac tcgagcaggg ctctggcgtc ccgtccggcg ccgtgccggc tcgaagggtt
     5161 gccgtagcgt tcggtcgcta cgccgaccgc ggcttcgagt atgtctcggt agggaggggc
     5221 ggtggccgcc cagtccagat agtgcatcgg gccatgatac atcggcgcgg acgggcgcgg
     5281 caagcgcgga cgtccgcctg tcccggctca ggagaagcgt cggtcgggaa taccacccgg
     5341 gacgcctccg gctgcggcgt agcctgcgcg aaggtacagc gcgcagaagc agtggccgcg
     5401 ctcggccacg tcgtcgcgcg cgtaggcgca ggggcagatg accgaggcgt cggcttcgcg
     5461 cgaaccgtcg gagtcccggc acgggcatag gaagtagccg taacggttcc agttgaccgt
     5521 caggccctcg atcaggctac ctacgaagtc ggcgtcaggg ttgagctccc agccctgctt
     5581 ggccgcgacc atcgtcgcga agcgcttcgt atcgtcgatg ctcttagcgg ccacagctag
     5641 actcccagag tccgcttcca gtcggcctcg ataaacccga caaggtagtc cttagcgccg
     5701 accgtcgcga aggggaaggc cacgtccgcc ttgtagatgt ccttgagtgc ccgcttcgcc
     5761 tcgttcttga cctcgatcgg gatggcgtcc atgtggatgt agcggtacgc gaagccgttc
     5821 gtctgcagga aggtcatggc gcgcttgcag aagccgcagg tcgacagcgc gtacacggtg
     5881 atgaccggca ccttcctcgg cccgtcgacg gcgatatagc tgagcgtttc taacatggct
     5941 tcgtcctcct gggaaccata gtacgcccgg agccgtcatt tggcaaatcg attgaataac
     6001 tggcccgatt cggtatacat tcgcgctatg cacgacatcg cccgaacccg caatttctgc
     6061 atcatagccc atatcgacca cggcaagtcg accctggccg accgcttcat cgagcgggcc
     6121 aagctcgtcg accagcgcaa gatgcagcag cagatgctcg acaacatgga catcgagcga
     6181 gagcggggca tcaccatcaa gagccaggcc gtcaccctgt cgtacgtcgc cagggacggg
     6241 ctcgagtaca ccctcaacct cgtcgacacc ccggggcacg tcgacttctc gtacgaggtg
     6301 tcgcgcgcca taagctcctg cgagggcgcc atactcctcg tcgatgccac ccagggcgtc
     6361 gaggcccaga ccctgtcgaa catgtacatg gccgtcgagc acgacctcga gatcctgccg
     6421 gtcatcaaca agatagacat gccggccgcc gacgtgcccg aggcgcgccg ccagatagac
     6481 cacgacctcg gcctggacgg tgacgaggcg ctctgcgtct cggccaagct cggcaccggc
     6541 ctcgacgagc tgttcgaggc catcgtcgcc cgggtgcccg cgccgagcgg ctccaaggac
     6601 gcccccctgc gcgccctgat cttcgacagc cactacgatc cctatcgcgg cgtcatcgtc
     6661 catatgcgcg tgttcgacgg gtccatcgtc cccggcatgg cgatccggct catgtacaac
     6721 gacgccgagt acgaggtcga ggagaccggc ctgttcaaga tgggcctcgt caagaccgac
     6781 ggcctcgtcg ccggcgacgt cggctacttc atcgccggca tcaagacggt ctccgacgtg
     6841 cgcgtcggag acacggtgac cggcgccgcg gacccggtcg ccgagccgct gccgggcttc
     6901 cgcgaggtca agccggtcgt ctacagctcc atctacccga tggacaccgc cgactacgac
     6961 gagctcaagg tggcgatgga gaagctcaag ctcaacgacg cgtcgctgat ctacgagaag
     7021 gacaactccg ccgccctcgg cttcggcttc cgctgcggct tcctcggcat gctccacctc
     7081 gaggtggtgc aggagcggct cgagcgtgag tacgaccagg ccatcatcat gaccgcgccg
     7141 agcgtgcgct accggctgac gatgagccac ggcggggata ccgtcatcat cgacaacccg
     7201 gccgactacc ccgacccgtc caggatagac ggcgccgagg agccgtacat caaggcgcag
     7261 atcatcacgc cgctcgcgta catcggcaac ctcatcacgc tctgcatcaa taagcggggc
     7321 tcgcagaccg gcttcaccca tctggacgag aagcgcgtcg agctgacctt cgagatgccg
     7381 ctcgccgagg tgctgttcga cttctacgac aagctcaaga gcacgagccg cggctacgcc
     7441 agcttcgact acgagatcat cgactaccgg ccgaccgagc tggccaagct cgatatcctg
     7501 ataaactcca agccggttga cgcgctcgcc cagctggtct atagaccttc ggcgatggat
     7561 cgtggccgca aggtctgcga gcggctcaag gactcgatgg atcgccagca gttcaagata
     7621 gccatccagg ccgcgatagg cggctcgatc atcgcgcgcg agacggtcaa cccggtccgt
     7681 aaggacgtgc tcgccaagtg ctacggcggt gacatcagcc gtaagcgcaa gctgctcgag
     7741 aagcagaagg aaggcaagaa gcgcatgaag atggtcggcg acgtcgagct gacccaggag
     7801 tcgttcatgg ccgtgctccg ggagaacgag gacgacgata ggcgctgaaa ggcggcgcgc
     7861 ggcaagcgcg cgcctcggcc tcgctccgct ccgccgagcc gttccgttcg cgtacggtcg
     7921 gccctggcga cgccggcggg gccgcctcta gctatcggcg gccatccgcg gtctcgatag
     7981 ccgcctcgag ccggcctcgc cagtacgcgg cctccgccgc gacgcgcctg agggcgtcgt
     8041 cctcgagggc cgtcacgcgc tccgggtctg ggaaatggca atacatgaaa tcagcctcga
     8101 ggatcagccc gcggagcgtc tgctcgtccg agccgccgcg tagcgccgcg gccacggtct
     8161 cggccagctc gagctcgccg cgcaggaagg ccgcggccct gagccggcag gcgccggggc
     8221 cggccgccac gcttatcgtc cccgccctcc gccccgccga cgcggagtcg cgcgcggatc
     8281 gtacggccgc gtcgatcacg gcctccgccg accccgcgac gtcagccagc ggggcgcgga
     8341 tgggcagggg tgcgccagcg ccgcagcgca ggtcgtaggc ttcgagacct cgcagctccg
     8401 aggccgccag ctcggcgtac atggacaggg cggggtcggt aagccattcc ccggatccgg
     8461 cgccaatcgg cctgacgccg tcacggaagc tcgcgttcca ggcggattca gccttgcata
     8521 gccggctctc gagtctcctt gagcggaggt cggccgggct acccgcgcaa tgtgtcctgc
     8581 cgggttcgaa cgcgaagtcc aggccggcga ccaggactgg tccgccgagc gccgaggcaa
     8641 gccgtacggc cagcacgccg accgagccga gcggcggcac ggccgtgagc ggcaggccgg
     8701 ccagggacag gcgcgacagg aaccgggtct cggcgtactc gctcaggacg aggctcgtcc
     8761 cgccggtcgc cgcgcggaag gtcgaggggt gggcgctcag gtccgccacg aagcgcggcc
     8821 tccgcccgcc gagcggcagg aagtcctcca gattgtagat ctgtccctcg aggcagacga
     8881 ccgcgtcggg cgtcacgccg gacagggcga gcgcgcccga cgcggtgtcg caggccagta
     8941 cgaatagctc gtcgcgcctg gctctcaggg cggggatcgc cgcatcgagg ctcgggccgg
     9001 cgccgcagac gacgatcggc ctcccgtccc cgcgtagcgg gcccacggag ggccagtcca
     9061 ccgcaccgag gttcgcgacg acgttcctcg tccagagtcg gcccatcctg
//
LOCUS       DAOPHV010000030         9101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_9542_length_9101_cov_3.813067, whole genome shotgun sequence.
ACCESSION   DAOPHV010000030 DAOPHV010000000
VERSION     DAOPHV010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9101
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9542_length_9101_cov_3.813067"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..486)
                     /locus_tag="PLQ29_01570"
     CDS             complement(145..486)
                     /locus_tag="PLQ29_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPG85360.1"
                     /translation="MRALIIDDDALNRHILRRYLAKLGYVCDEAASGLEGLDACRSAN
                     YDVALVDLILPDLDGSDTATAIREAYASRATPVRLIAVTGARCDERVKRMFDAVVEKP
                     YTLEELSAVIV"
     gene            complement(483..3167)
                     /locus_tag="PLQ29_01575"
     CDS             complement(483..3167)
                     /locus_tag="PLQ29_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPG85361.1"
                     /translation="MRPIVAIAVRNPGVLEHVSRELRSRVTGAEITTIVGDRAFRETI
                     ARGAAASIDALVADAECAELIAALSKPEDLPLVGVIAPASSGAGLPRAIADAGFPMLR
                     LCAEPRPYAEVAAWVNGAVASIARAGGRAHDSVDYGQRYEDLVQALPDIVYELDVDGV
                     ITFINDSVSMLGYAPGDLIGKHYSVLLHDEDAAAVDRDRVLVDYVGYRTGVALSPKLF
                     NERRSIERRTTDLEVRLKRKRGSKNGLPELIGSVISYGEISSAGEYGRDGAREFKGSV
                     GIIRDVTLRRKSEETLRKLYQAIDQLGSCVFVLNHAFEVEYVNPAFFMLTGFAPPDVI
                     GGNVFRFLAFLPDTVDAVRKRVQDGFEAREEALVPRMGGGQFWASLSIAPVRTPSGAI
                     THAIAVMDDISARRSMEELLKGARLEAEEASRSKSRFISSMTHELKTPIASIIAAARS
                     LQGGEGGPVALILDNALRLLDILNGILDYVRSESDDGAVRRVSFRLGSFMERACGPHR
                     ATAVGKGLSFSLEADSDESVESDPDRLGRVIEILLDNAVRYTDSGSIRVTARIERLEG
                     NVPHLIVHVADTGPGIAAQDRERIFKPFSRAEHAARGGPGGSGVGLALARNIMQILGG
                     EVRLDTGFGIGSDFSLILPTASPVRTEAAEAAGYTLLLVDDNEVNLEYMRTLVENRGF
                     RVLCAESAAEAFRVLEAHYVDACILDIQMPGYSGIELAKGIRAYAGPRYSSDLPLFAM
                     TAHDTAALGGDEGLFAAVFPKPTDIAKLSDTVIATMAEQDRVASSAFPMAFCATGFGR
                     TAAVARMRLEVESAMTALILALSGTSDARIDIRAEAASLSSVFNRFSCKSGQAKVRLF
                     IEHYADDDRDALRCLLDRIGRILGAGIDSIANLPGGRE"
     gene            complement(3198..5159)
                     /locus_tag="PLQ29_01580"
     CDS             complement(3198..5159)
                     /locus_tag="PLQ29_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPG85362.1"
                     /translation="MMRYPAGDERLFGISVPLGSIKVHGGWRVGEYPDLVPFAGLCKE
                     LGARLLQLLPLNDTGGQSSPYSALSAFALHPLYIRVRDLPEAAAAPGAVEELESFAAK
                     ATSGERFPYGACLDAKLAALRAVYKASADSIAADAGLASFESDRPWLRTYAVYRRLKS
                     SYGEQAWRSWPDHGRPTRGEIDTLWDDPSARLEQLFHAWMQYRAAEQFGAAAEAVAGM
                     GLVLLGDIPILMNDDSADVWADRGFFDDSLRAGAPPDMYSAVGQNWGFPIYDWAAMAE
                     DGYSFWRARVAEAARYYSAYRIDHVLGFFRIWALGDRDETGCLGRFVPGPELGADELT
                     RSGFSEARLRWLSEPHIPGAELRVAVPATEAASVVDAALERIGDEDLFRFKRSIRGEA
                     DIGALPLGGATRDYLKDRWRDRALLAIGGGRFAPTWSYRGTRAWASLSDGERSVLEAM
                     LGIASASAELDWEDRGRRLLSMLGSASDMLPCAEDLGAVPDCVPRVLSELGIPGLRVP
                     RWMRRWDEPGQPFMPLSEYGELSACTPSVHDTSTLRGWWEREDGRDAFVEAYCPELSP
                     ASALLDPEREGAVLAALAKAPSKLFVVQLQDLLDLSDRYRSDDPSDDRVNVPGSVDEF
                     NWTWRMGPGAEELERDRAWTALVRAGCGR"
     gene            complement(5156..6556)
                     /gene="radA"
                     /locus_tag="PLQ29_01585"
     CDS             complement(5156..6556)
                     /gene="radA"
                     /locus_tag="PLQ29_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPG85363.1"
                     /translation="MQSVVAKAPRTSYECSSCGHREAKWLGRCPACGEWNAMRETSAP
                     AGDGGPKREARFSVPLESVDPAEGARSPSGSAEVDRVLGGGIMRGSSVLIGGEPGIGK
                     STLLLQLAARASVQGRVLYVSAEESAAQIRLRADRLGLKRPGLELLCTGDLSEAVRAL
                     DRVKPALFVVDSLQTIASADAGNVPGTANQVKYCALELADWAREHGSACFLVAHVTKD
                     GMIAGPKAAEHTVDVVLMFEQSETDARFLRSAKNRFGSTDEVGLFTMTERGLEEQTDP
                     SELFLVRRAGEAPAGAAVAATWEGTRALLVEIQALTVPGKASLSRVYSERVEPARVAR
                     VAAVLEKHAGLGLSDQDLYVNVSGGMRLSEPGIDLALAAAIYSARTGLALPPSAALAG
                     ELSLAGEVRPVRRMSGRVRAARAMGFERVVGAASIRGEATVRGEADDPDSWERVSTIG
                     ATIRALFGSGAKNGGR"
     gene            complement(6582..8363)
                     /locus_tag="PLQ29_01590"
     CDS             complement(6582..8363)
                     /locus_tag="PLQ29_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPG85364.1"
                     /translation="MARIRLSGALALAAVMATLAGCAGAPKTEGSAPAPKPAASARDT
                     PSAELGVTKEARGEAAPPPAVMYDYGAAKPSSGAAGSRGVTPSSSGLKAGYSDDNEQF
                     NYFVGFLEKYADVPHYGYDISERITVRVLDSAGKPVANAAVSIRSGGKELAAGLSYGD
                     GSFRFYPAAIGSKDATFELRASAGGLSAGATTRRDGPRTVELRLQGPRSLPDPLPLDL
                     LFVMDTTGSMGEEIERLKATIEIIHDNRAALRPRPLVRLGLVLYKDLGDEYVTRLSPF
                     TADLETFRAALQPVVASGGGDGPEDLESALDDAVNRMDWNEGGLRLAFVVTDAEAHLD
                     YGRDYTYVDAANDARARAIKLYTIGTGGLPLEGEYLLRQVSQLTDARYIFLTYGEAGE
                     SDGGAEGSVSHHTGSNYQTDKLEAIVIRFVKEEVAWLSDTPVSVDEGYFSARPVDEES
                     RDQTLGKLFVESVGNLADYSTFRFGPEAPCVILPVSASGDGLGPSAEYFGERLAMAAS
                     EAGRWKPVERKELQRVLAELELQLSGVADPATAARAGELLGADLAVASSLYLRDGRYE
                     LFMRLVRVSTAEVLSVARAKIEKDLGL"
     gene            complement(8439..8729)
                     /locus_tag="PLQ29_01595"
     CDS             complement(8439..8729)
                     /locus_tag="PLQ29_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85365.1"
                     /translation="MISREDFMFTIGYDGDTAIVDGKAKAQYGKLDTLSLARRGLFRS
                     SFASALYSGSKEELTAFAELYNAASGTSYSTAEEFQRLFGVKREAVRKVLVL"
     gene            complement(8818..8976)
                     /locus_tag="PLQ29_01600"
     CDS             complement(8818..8976)
                     /locus_tag="PLQ29_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPG85366.1"
                     /translation="MEKYICDVCGYIYDPAVGDPDAGVAAGTSFAKLPDDWVCPMCGA
                     GKDNFSLA"
     gene            complement(8992..9096)
                     /locus_tag="PLQ29_01605"
     CDS             complement(8992..9096)
                     /locus_tag="PLQ29_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HPG85367.1"
                     /translation="MKPGDKPEAVFGVSATHVVAREYCNLHGLWKAEA"
BASE COUNT         1544 a         3109 c         2938 g         1510 t
ORIGIN      
        1 ggactcgaac ctacgacctc cgggttatga gcccgacgag ctgccaactg ctctaccctg
       61 cgtcgatctg tgattaagat acagaaaacg ataaagaatg tcaatagact acaagctttg
      121 aactcttgat tgcagcttgg atgcctatac aataaccgcc gacagctcct cgagggtata
      181 cggtttctcg acgacggcgt cgaacatgcg cttgacccgt tcgtcgcagc gcgcgcccgt
      241 gacggcgatc agccgtacag gcgtagcccg cgacgcatag gcctcacgga tcgccgtagc
      301 ggtatcggag ccgtcaaggt ccggcagaat gagatcgacg agagctacgt cgtagttggc
      361 gctacgacag gcgtccaagc cctcgagccc actggccgcc tcatcgcaga cataccctag
      421 cttggctaga tacctgcgta gtatatgccg gttcaaggca tcatcgtcga tgatcagcgc
      481 cctcattcac ggccgcctgg caggttagcg atcgaatcga tgcccgcccc taggatacgc
      541 cctatcctat cgagtaagca gcgtaaggcg tcccggtcgt cgtcagcata atgctctatg
      601 aacagcctta ccttggcctg gcccgacttg cacgagaagc gattaaagac cgacgatagg
      661 gaggccgcct cggcccgtat gtctatccgt gcgtcgctgg tacccgacaa agccaggatt
      721 agggcggtca tggccgattc cacctcgagg cgcatcctcg ccacggcggc cgtgcggccg
      781 aaacccgtcg cgcagaacgc catagggaag gccgagctgg ctaccctatc ctgttcggcc
      841 atcgtagcga tgacggtgtc cgaaagcttg gcgatgtcgg tcggcttcgg gaagacggcg
      901 gcgaacagcc cctcgtcgcc gccgagggcg gccgtatcgt gggcggtcat ggcgaacagg
      961 ggcaggtcgg aggagtaccg tggaccggcg taagcgcgga tgcccttggc cagctcgatg
     1021 ccggaatagc cgggcatctg gatatcgagg atgcaggcgt cgacgtaatg ggcttccaac
     1081 acacggaacg cctccgccgc gctctcggcg caaagcacgc ggaagccgcg gttctccacc
     1141 agggtcctca tgtactcgag gttcacctcg ttatcatcga cgaggagtag cgtatagccg
     1201 gccgcctccg ccgcttccgt cctgaccggc gaagcggtcg gcaggatcag cgagaaatcg
     1261 ctgcctatgc caaaaccggt atcgagccgt acctcaccac cgaggatctg catgatgttc
     1321 ctggcgagcg cgagaccgac gccagaacct ccggggccgc ctcgggccgc gtgctccgcc
     1381 ctggagaagg gtttgaatat gcgctcccta tcctgagccg ctatgcccgg tcccgtatcg
     1441 gccacgtgga cgatcaggtg cggcacgtta ccttcgagtc gctcgattct ggccgttacc
     1501 cgtatcgagc ccgagtcggt gtagcgcacc gcgttatcga gcagtatctc gatgacgcgg
     1561 ccgagtctgt ccgggtcgct ctcgacgctc tcgtccgagt ccgcctcgag cgagaaagat
     1621 agccctttcc ccacggccgt cgcgcgatgt gggccgcagg cccgctccat gaacgagccg
     1681 agccggaagg agacacgtcg tacggccccg tcgtcgctct ccgaacggac gtaatcgagt
     1741 atgccgttca gtatatcgag gagacgtagg gcattgtcga ggatgagcgc tacgggaccg
     1801 ccctcgccgc cctggagcga acgagcggcg gcgatgatgc tcgcgatcgg cgtcttaagc
     1861 tcgtgggtca tgctcgagat gaagcgcgac ttcgatcggc tcgcctcctc cgcttcgaga
     1921 cgcgcgccct tcagtagctc ctccatggac cgcctagcgc tgatgtcgtc catgacggcg
     1981 atggcgtggg tgatcgcccc ggacggcgtc ctgaccggcg caatcgacag ggacgcccag
     2041 aactgtccgc cgcccatacg cgggacgaga gcctcttccc gcgcctcgaa gccgtcctgg
     2101 acccgcttgc ggacggcgtc gaccgtatcc ggcaggaagg ccaggaacct gaagacgttg
     2161 ccgcctatca cgtccggcgg cgcgaagccc gtcagcatga agaaggcagg gttgacgtac
     2221 tcgacctcga acgcatggtt gagcacgaag acgcacgagc cgagctggtc gatcgcctgg
     2281 tacagcttgc ggagcgtctc ctccgacttg cgccgtagcg tgacgtcgcg gattatgccg
     2341 accgagccct tgaattccct ggctccgtcg cgaccgtatt cgccggccga cgagatctcg
     2401 ccgtacgaga tgacgctgcc tatcagctcg gggagcccgt tcttggagcc gcgtttgcgc
     2461 ttcagcctga cctcgagatc ggtcgtacgc cgctcgatgc tacggcgctc gttgaacagc
     2521 ttcgggctga gggcgacgcc ggtccggtaa ccgacgtagt cgaccaggac cctatcccgg
     2581 tccacggccg ctgcgtcctc gtcatggagc aggaccgagt agtgcttgcc tatgagatcg
     2641 cccggggcgt agccgagcat ggagaccgag tcgttaatga aggtgatgac gccatcgacg
     2701 tcgagctcgt agacgatgtc gggcaaggcc tggaccaggt cctcgtatcg ttgcccgtag
     2761 tcgaccgaat cgtgggcgcg ccccccggcc ctggctatcg acgcgacggc gccgttcacc
     2821 caggccgcga cctcggcgta gggcctcggc tcggcgcata gccgtagcat ggggaaaccg
     2881 gcgtccgcga tagcccgagg caggccggca ccgctcgaag cgggcgcgat gactccgacc
     2941 agcggcaggt cctcgggctt cgatagcgcc gctatcagct cggcgcactc cgcgtccgcc
     3001 acgagcgcgt ctatggacgc ggcggcgccg cgcgctatcg tctcgcggaa agccctgtcg
     3061 ccgactatcg tcgttatctc ggctcccgtc acccttgagc ggagctcgcg cgagacgtgc
     3121 tcgaggactc ccggatttct gaccgctatg gcgacgatcg gacgcatcgc atcttttgta
     3181 ctctgagcca tactacctta tcggccgcag ccggcccgta cgagagcggt ccacgcacgg
     3241 tcgcgctcga gttcctccgc gccgggcccc atgcgccagg tccagttgaa ctcgtccacg
     3301 ctgcccggca cgttgaccct atcgtcgctc ggatcgtccg acctatagcg atcggatagg
     3361 tcgagcaggt cctggagctg gacgacgaac aacttggacg gggccttcgc cagcgccgcg
     3421 agcacggcac cttcacgctc cgggtcgagt agcgccgaag cgggactcag ctcgggacag
     3481 taggcctcga cgaaggcgtc cctgccgtcc tcccgttccc accagccgcg aagcgtcgag
     3541 gtatcgtgga cgctcggcgt gcaggcggat agctccccgt attcggacag cggcatgaac
     3601 ggctggcccg gctcgtccca tcgcctcatc cagcgcggta cccgtagccc gggtatgccg
     3661 agttcggaga gcaccctcgg cacgcagtcg ggaacggcgc cgagatcctc tgcgcagggc
     3721 aacatgtcgg aggccgagcc gagcatggac aggagccggc ggccgcgatc ctcccagtcg
     3781 agctcggccg acgccgaagc gatgccgagc atggcctcga gcaccgaccg ctcgccgtcc
     3841 gagaggctcg cccaggccct ggttccgcga tatgaccagg ttggcgcgaa gcgtcctccg
     3901 cctatggcca gcagagccct atcacgccat ctgtccttga gataatccct ggtggctccg
     3961 cccagaggca gggcgcctat atctgcctcg ccccgtatcg atcgcttgaa cctgaatagg
     4021 tcttcgtcgc cgatgcgctc gagcgccgca tcgacgacgc tcgcggcctc cgttgctggt
     4081 acggcaactc ggagctcggc gccgggtata tgcggctcgg acagccagcg tagccttgct
     4141 tccgagaagc cagaccgcgt cagctcgtcc gccccgagct cgggtccggg gacgaagcgg
     4201 ccgaggcagc cagtctcgtc ccggtcgccg agcgcccata tcctgaagaa cccgagaacg
     4261 tggtcgatgc ggtaggccga gtagtacctg gcggcctccg cgacgcgcgc tctccagaag
     4321 gaatagccgt cctccgccat ggcggcccag tcatagatcg ggaagcccca attctggccg
     4381 accgccgaat acatgtccgg aggcgcgccg gcccggagcg aatcgtcgaa gaaaccgcga
     4441 tccgcccaga cgtcggccga gtcgtcgttc atcaggatgg gtatgtcccc taggaggacg
     4501 aggcccattc cggccactgc ctcggctgcc gcgccgaact gttcggcggc ccgatactgc
     4561 atccaggcgt ggaagagttg ctcaagccta gcggaggggt cgtcccacaa cgtgtcgatc
     4621 tcgcctcggg tcggccgccc gtggtcgggc cacgagcgcc aggcttgctc gccataggac
     4681 gacttaagcc gccggtagac ggcgtaggta cgcagccacg ggcgatcgct ctcgaaggag
     4741 gccaagccgg cgtcagcggc tatgctatcg gccgacgcct tgtagacggc cctgagcgct
     4801 gccagcttgg cgtcgaggca cgcgccgtat gggaaacgct cgcccgaggt cgccttggcg
     4861 gcgaacgact ccagctcctc gactgcccca ggcgccgcgg ccgcctccgg caggtcccga
     4921 accctgatgt agagcgggtg tagcgcgaag gccgacagcg cggagtaggg cgagctctgg
     4981 ccgccggtgt cgttgagcgg aagcagctgg agcagcctcg cgccgagctc cttgcatagc
     5041 ccggcgaacg gcacgaggtc cgggtactcg ccgaccctcc agccgccgtg caccttgatc
     5101 gacccgagcg gaaccgagat cccgaacaat cgctcgtcgc cggcggggta acgcatcatc
     5161 gtcctccgtt cttggcgccc gagccgaaga gggcccgtat cgtcgcgcct atcgtcgaga
     5221 cccgttccca agagtccggg tcgtcagcct cgccccgtac cgtggcctcg ccccgaatgg
     5281 aggcggcgcc gaccacccgc tcgaacccca tcgccctggc cgcccgaacg cggccggaca
     5341 tgcgccgcac cggccggacc tcgcccgcga gcgacagctc cccggcgagc gcagcggacg
     5401 gcggcaacgc cagccccgta cgcgccgagt agatcgcggc cgcgagcgcc aggtctatgc
     5461 ccggttccga taggcgcatg ccgcccgaga cgttgacgta caggtcctgg tctgaaaggc
     5521 cgaggcccgc gtgcttctcc agcacggcgg ccacgcgcgc cacccgcgcc ggctcgacgc
     5581 gctcggagta gacgcgtgac aaagacgcct tgcccggcac ggtcaacgcc tggatctcca
     5641 ccagcagggc gcgcgtcccc tcccaggtag ccgccacggc cgctcccgcc ggcgcctcgc
     5701 cagctcggcg cacgaggaac agctcggacg ggtcggtctg ttcttcgagc ccacgctcgg
     5761 tcatcgtgaa cagccctacc tcgtcggtcg agccgaatcg gtttttggcc gagcgtagga
     5821 aacgcgcgtc ggtctcgctc tgctcgaaca tcagcaccac gtcgacggta tgctccgcgg
     5881 ccttgggccc cgctatcatt ccgtccttcg tcacgtgggc gacgaggaaa caggcgctgc
     5941 cgtgctccct ggcccagtcg gccagctcga gggcgcagta cttgacctgg ttggcggtgc
     6001 ccggcacgtt gccggcgtcc gccgaggcta tcgtctggag cgagtcgacg acgaacagcg
     6061 ccggcttgac ccggtcgagc gcccgaacgg cctcggacag gtcgcctgtg cagaggagct
     6121 cgagccccgg gcgcttgagg ccgagcctgt cggcgcgtag ccgtatctgg gcggcgctct
     6181 cctcggccga cacgtacagg acgcggccct gcacgctcgc cctggcggcc aactgcaaga
     6241 gcaacgtgct cttgcctatg cccggctcgc cgcctatgag cacgctcgaa ccccgcatga
     6301 taccgccccc gagcacgcga tccacctctg ccgagcctga aggcgagcgg gcgccctcgg
     6361 ccgggtcgac cgactcgagc ggcacggaga agcgcgcctc ccgcttgggg ccgccgtcgc
     6421 cagctggcgc gctcgtctcg cgcatcgcgt tccactcgcc gcaggccggg caacgcccga
     6481 gccacttggc ttcgcgatgt ccgcaggagg aacactcgta gctcgtcctg ggggccttgg
     6541 ctaccacgga ttgcacccgc tcgcctcgtc tgccgacgga gtcataggcc caggtccttt
     6601 tcgattttag cccgggcgac ggacaacacc tcggcagtcg aaacccgcac gaggcgcatg
     6661 aacagctcgt agcggccgtc gcgcaggtat agcgacgacg ccacggccag gtcggcgccg
     6721 agcagctcgc cggcgcgggc cgccgtagcc ggatcggcta caccggacag ctgtagctcg
     6781 agctccgcga gcacgcgctg cagctcctta cgctcgaccg gcttccagcg gccggcctcg
     6841 gacgccgcca tcgcgagccg ctcgccgaag tactcggcgc tcggcccgag gccatcgccg
     6901 ctcgccgaca cgggcagtat gacgcagggc gcctctggcc cgaaacggaa ggtagagtag
     6961 tcggccaggt tgccgacaga ctcgacgaac agcttgccga gtgtctggtc tcggctctcc
     7021 tcgtcgaccg gcctcgcgct gaagtagccc tcgtcgaccg aaacgggcgt gtccgacagc
     7081 caggcaacct cctccttgac gaagcggatg acgatggcct cgagcttgtc ggtctgataa
     7141 ttcgagccgg tatggtggct tacggagccc tcggcgccgc catccgactc tcccgcctcg
     7201 ccgtaggtga ggaagatgta cctggcgtcg gtcagctgcg atacctgcct gagcagatac
     7261 tccccctcga gcggcaggcc gccggtgcca atcgtataga gcttgatggc cctggcccgc
     7321 gcgtcgttgg ccgcgtcgac gtaggtatag tcgcggccgt agtccagatg cgcttccgcg
     7381 tcggtgacga cgaaggccag cctgaggcct ccctcgttcc agtccatgcg gtttaccgcg
     7441 tcgtccaggg cggactccag atcctcgggg ccgtccccgc ccccggaagc gacgaccggc
     7501 tggagggcgg cgcggaacgt ttcgagatcg gcggtgaacg gcgagagcct cgtcacgtac
     7561 tcatccccta ggtccttgta tagcacgagg ccgagacgca cgagcggtcg cgggcgtagc
     7621 gccgcccggt tgtcgtggat aatctcgatg gtcgccttga gccgctctat ctcctcgccc
     7681 atcgacccgg tcgtatccat cacgaacagt aggtcgaggg ggagtgggtc gggcaacgaa
     7741 cgcggtccct ggagccggag ctcgacggtg cgcgggccgt cccgcctcgt cgtcgcgccg
     7801 gcgctcagcc ctcccgcgga ggccctcagc tcgaaggtcg catccttcga gccgatcgcc
     7861 gcggggtaga agcggaaaga tccgtcgccg tagctcaaac cggccgccag ttccttgccg
     7921 cccgagcgta tcgatacggc ggcgttcgcc accggcttgc cggccgagtc gagcacccgc
     7981 acggttatgc gctccgagat gtcgtagccg tagtgcggca cgtcggcata cttctcgagg
     8041 aagccgacga agtagttgaa ctgctcgtta tcgtcggaat agccggcctt gaggccggac
     8101 gaagaaggag tgacgccgcg cgatcccgcg gctcccgacg acggcttggc tgcgccgtag
     8161 tcgtacatga cggcgggcgg cggggcggcc tctcccctcg cctccttcgt gacgccgagc
     8221 tcggctgagg gcgtatcgcg ggcagaggcg gcgggttttg gagccggggc gcttccttcg
     8281 gtcttcgggg cgccggcaca tccggctaga gtggccatga cggccgcgag cgcgagcgcg
     8341 ccgctcagtc tgatacgagc catagggagc ctcctaaact aatagcttca gtatactacg
     8401 aaggaggccg cctcgccgct ggacctagcg ctctcccgct agagaaccaa gaccttcctt
     8461 acggcttcac gcttgacgcc gaacaggcgc tggaattcct ccgcggtaga gtacgaggta
     8521 ccggaggccg cgttgtagag ctcggcgaag gcagtgagct cttctttgga gccggaatac
     8581 agggccgacg cgaaggagga tctgaacagt ccccggcgag ccagcgatag ggtatcaagc
     8641 ttaccatact gggccttggc cttgccgtcg acgatggccg tatcgccgtc gtagccgatc
     8701 gtgaacatga agtcttccct ggatatcatc acgcctccga aaaaaagagc cggaaccaac
     8761 cctcgggctg gcttccggct tcgatgataa cgcgccgcga gccggcggcg caacgggtta
     8821 ggccaggctg aagttatcct tgccggctcc gcacatcggg cagacccagt catccggcag
     8881 cttggcgaac gaggtgccgg cggccacgcc agcgtcaggg tcgccgaccg ccgggtcgta
     8941 gatgtagccg catacgtcgc agatgtactt ctccatcgtt ttctcctctt cctatgcctc
     9001 ggccttccag aggccgtgca ggttgcaata ctcgcgggcg actacgtggg tcgccgagac
     9061 gccaaagaca gcttcgggct tgtctccggg cttcaggtag g
//
LOCUS       DAOPHV010000031         8912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_9837_length_8912_cov_3.533025, whole genome shotgun sequence.
ACCESSION   DAOPHV010000031 DAOPHV010000000
VERSION     DAOPHV010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8912
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9837_length_8912_cov_3.533025"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1928)
                     /locus_tag="PLQ29_01610"
     CDS             complement(<1..1928)
                     /locus_tag="PLQ29_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85368.1"
                     /translation="MRTKPFILVGLYCIALFISCDFKTTIFGDFNSPNDPRTTEYADY
                     TIEVLAGIGSNPSPETTDATTLRLSHVTDLEASGGYLYVADSLHHRVIRIHETSGAAS
                     AIAGTGVPGYSGDGGPAASARLFAPTAVLAWDDGSLFILDAMNDAVRRVSADGVISTY
                     ADLAALTGEKADLWMQADWNRGCMARLQNDILVYHYHRIYRLSGTIGSVSAVEYPATD
                     FFGTIGGDPNSFGVDDGGALWLSAWLHESQKRVLGRVASGSTEFEVMREFDYGASDSI
                     DFDSSGKVLAYKDQDEIIAYDPSDGSVTNRTDEDGGSVFCINSSGVLYRYNVWCDLGQ
                     HVITKMTAPLTAPAVFAGIASLVPTGDPTDRIQPWHLALDEEDGELYYTDTSQTIVRS
                     VNVSTGQGTLVASGLPGSPKGVSIGPGGEIVFSNNGDSNIYRLSGSSYSLIATYDSPL
                     EDVAVAPNGAVFAASFTFSPSGGFTYFTGLVKRVSGEHSILRVGNGTPIAYADPDGSY
                     STAIPIGAVWAICFGPDGTLYLAEEERRILAVAPTGVVTRIAGGIWTETSPDRVWFDS
                     FEIEDEFMKAREASIRNSPRQIAYGADGCLYIAHQYDAVLRVNAESRIEQLRFHLDGR
                     TVDLCARGIGRPSVRPNG"
     gene            complement(1925..2386)
                     /locus_tag="PLQ29_01615"
     CDS             complement(1925..2386)
                     /locus_tag="PLQ29_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85369.1"
                     /translation="MFKQEIEIVPGSEQSLSIPHLDFSNSWKISDLTIQRSLAFDRLV
                     AIQKARKSRAAISNLGAVIGLAGLGVAVYGIVDGTSAYPEYQVAATPAEALLLRQRLV
                     LDQNYLVFGLAGSGASFLLWTVRFGKQEELRNAQDEIDRLERMIDELRSQE"
     gene            complement(2576..4294)
                     /locus_tag="PLQ29_01620"
     CDS             complement(2576..4294)
                     /locus_tag="PLQ29_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPG85370.1"
                     /translation="MLIAAQLLVAQDRGLAVQARQVTGDPSFDIGRQYAVIIGIDRYQ
                     EWNSLRNAVSEARNVKAILESRYYIDEFIELYDGAATAAGIRKLFAETLPRKIRKEDS
                     LFIFYAGHGYLDSSNTGFWIASDGVKDVFDQRGWIPNQQIRNYLSLLKAQRVLVVADA
                     CFSGDFLNVSRGAGPTIDSAYFAKALKLQARQVLTSGASETVPDESEFGRYFVTTLER
                     NADPILDPHSLYDRIRSNVTKTVPLLGTIPGNEAGASFAFFLRPSYGSVSVAVATSAN
                     VEVDGRGLGAAEPGKPLLLKDLSAGIHELRVIYGDSEERRSILVRSGETSSEGFDYRA
                     PRFGSLTVSAQAEATVLVDGKPVGSIAAGSTLVLPGVAEGERVVTLQYSDNQETRKLV
                     VYPDTANAVSFAYRQESFASVAFEGFPDGAVIYSAGRELGTIAAGRFTAGGLAPGPRS
                     FSVYHESWASPYLVAVDAAARSTATAAFKGGRIVALSVPRNVQIAVDGKVVARGDGLR
                     DEYDLGLFPEGSHSVSFTGDNWDQQRSTASVAHGATVRVTASMTMRPGSSGGQATPSG
                     TAASGS"
     gene            complement(4495..5049)
                     /locus_tag="PLQ29_01625"
     CDS             complement(4495..5049)
                     /locus_tag="PLQ29_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPG85371.1"
                     /translation="MKGNRMYVDIGSMLDEIFDAAKNFGDQMKDNFGPGGPGCATGPG
                     GQRSNPWFGMMDQDENSDWYPSFSYPPMNIFLTQDRSMVFEFALSGFDENDISLSFQG
                     DYMVFSARIGLEAAPEEGVRYFKRRLKLKDIEKQKYYVPAEKFDQAMVKAVFKNGILR
                     VTVPPKESDEINEGIKIEIVKEGE"
     gene            complement(5258..7138)
                     /locus_tag="PLQ29_01630"
     CDS             complement(5258..7138)
                     /locus_tag="PLQ29_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPG85372.1"
                     /translation="MRKSARTILAALAALAIVLAFGSCAHPFEQFAETPLKITFIETT
                     DIHGAIFPYNFITGKAMDTSMAQVATLVGQKRAEGGEIVLMDDGDVLQGQPTVYYYNF
                     EKTDVPHIWSEVLNYLQYDVATIGNHDIEAGHAVYDKLAKEVKAPMICANINKEGTDE
                     PYFPPYAIITRQGVKIAVLGMIEPKITEQLPKAFWEGMEVLDMVETAKRWVPIILEKE
                     KPDLLIGLFHAGVDYTYGGQTKATPKNESASQLIIEQVAGFDLVFVGHDHAGWEGKGY
                     NPETKAKDVAVMDPTGKVVPIYGALSGAQNVAVVDVTMTWDKEAKTWKKDIAGSLVPL
                     KGVAADPAFLAKFEPAKAELMDWVERPIGKMVGSINSDEAMFGDSAFVDLINRIQLEL
                     CADATMGLKPAQISIAAPLDQYAYLPTMQDGTIKVRDMFSLYKYENFMYTMDLTGEQI
                     VKFMEFNYGNQFNTMTGPQDQLIAFVKDKEGKPVYNARYNNYDTITRPYNYDAVAGIK
                     YTVDVSKPAGSRIKVISMADGSPFELGRTYSVAINSYRGSGGGGHLTKGVGLPAEKLQ
                     KLELVTSSTTKDLRFYMLKWFEKQTGPITVTRDDNWKVIPEDWAEAGKANSYPLMYPP
                     KK"
     gene            7350..8129
                     /locus_tag="PLQ29_01635"
     CDS             7350..8129
                     /locus_tag="PLQ29_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85373.1"
                     /translation="MGGRLDRFLSAYDQEGLQLRKKARMVVMTCVAGSVAAALMTATR
                     AVLAFDIVSLAMGLAMIASAIGCALLVRSGRYTFGVNFIMGMFSVGFFALSAVRPAEN
                     ASFLYLSAVYDAFLLSALCLIAIRTYQVAMFTVGYVASLVTLFLLKRASIVDPAALAP
                     VDLVQAVVFTALTGTVGFLVMRMTNGTIVIAEAEAAANEDRAEGIKAVVANLHRGLDS
                     GNDLLAASSSALASLQAVELAVAAYEPRLPVSSTRRIKPGG"
     gene            8126..>8912
                     /locus_tag="PLQ29_01640"
     CDS             8126..>8912
                     /locus_tag="PLQ29_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG85374.1"
                     /translation="MSAETTGKVAAAKSDLAAHDGAVRSAVASVAAIGESIGRLSTLA
                     ARGRDAMTSLVGAASEGERSIDDANAALAAVEKASGEMLGIVNVIAGIASQTNLLAMN
                     AAIEAAHAGAAGKGFAVVADEIRKLAEETDQNSRSIADSLKRNSAGVASSIKTSRLAR
                     ASFGRIAAEVGEANGSMGGILEGLDGVRAEASSLDGDLRRVSEASRAVAASMAGVEAM
                     AAESGSGLSAIFKGAESIASKAAEIASEYGRIAAEMEKVDGIGK"
BASE COUNT         1495 a         2737 c         2917 g         1763 t
ORIGIN      
        1 cttccatttg gacggacgga cggtcgacct atccccctgg cgcataggtc gaccgtccgt
       61 ccgtccaaat ggaagcgtag ctgctcgatc cggctctcgg cgttgacgcg tagcaccgcg
      121 tcgtactggt gcgctatata gaggcacccg tcagcgccat acgcgatctg cctcggcgag
      181 ttgcgtatcg aggcttccct ggccttcatg aattcatcct cgatctcgaa cgagtcgaac
      241 cagacgcgat cgggactggt ctcggtccag atgccgccgg cgatgcgtgt aaccacgcca
      301 gtgggcgcga cggccagaat ccgccgttct tcctctgcga ggtagagcgt cccatcgggc
      361 ccgaagcata tcgcccatac cgccccgatg ggtatagccg tggaatagct accgtcaggg
      421 tcggcatacg ctattggcgt gccgttcccc accctgagta tggagtgctc cccggacacg
      481 cgtttgacca ggcctgtaaa gtaggtgaat cctccggacg gtgaaaaggt gaacgaggcg
      541 gcgaagaccg ccccgttcgg cgctacggcc acgtcctcga gcggactatc gtacgtggcg
      601 atcaacgagt agctactccc cgaaagccta tagatattcg agtcaccgtt attcgagaag
      661 actatttccc cacccgggcc gatggagacc cccttgggcg atcccggaag gccggaagcg
      721 acgagggtcc cctggcccgt cgagacgtta acgctgcgta ctatggtctg gctagtatcc
      781 gtataataga gttccccatc ctcttcgtcc aaggccaggt gccagggttg gattctgtcc
      841 gtcgggtcgc cggtgggaac caaggaagcg atcccggcga atacggctgg ggcggtcagg
      901 ggggcggtca tcttggttat gacgtgttgg cctagatcgc accaaacgtt gtagcggtat
      961 agcacgccgg agctattgat acagaataca gagccaccgt cttcgtcggt acggttggtc
     1021 acggagccgt ccgatggatc gtaggcgata atctcgtctt gatctttgta ggcgagaacc
     1081 ttgcccgatg aatcgaagtc tattgaatcc gatgctccat agtcgaattc gcgcatgacc
     1141 tcgaattcgg tcgagccgct cgctactctg cccaagaccc gcttctggct ctcatgtaac
     1201 caggccgata gccatagcgc tccgccgtcg tcgacgccga acgaattcgg gtcacccccg
     1261 atggtgccga agaaatcggt ggcggggtac tcgaccgcgc tgaccgaacc gatcgtgccg
     1321 cttaaccggt agatgcgatg gtagtgatac accaggatgt cgttctgcag ccgggccatg
     1381 cagcccctgt tccagtcagc ctgcatccaa aggtccgctt tctcgcccgt cagcgcggcc
     1441 aggtcggcgt aggtgctgat gacgccgtcc gcgctcaccc tcctgacggc gtcgttcatg
     1501 gcgtccagta tgaacagcga tccgtcgtcc caggccagca cggccgtcgg agcgaagagc
     1561 ctggccgagg cggccgggcc tccgtcgccg gaatatcccg ggacgccggt ccccgctatc
     1621 gccgatgccg cgccgctcgt ctcgtgtatc ctaatcaccc ggtgatgcaa cgagtcggcg
     1681 acgtacagat agccgcccga cgcttccagg tcggtgacgt gggacaggcg caacgtcgtc
     1741 gcgtcggtcg tctccggaga agggttcgag cctatgccgg ctagtacctc gatcgtgtag
     1801 tctgcgtact cggtggtgcg cgggtcgttc ggactgttga agtcgccaaa gatggtggtc
     1861 ttaaaatcgc aggatataaa gagcgcgatg caatacaggc cgacgagtat gaacggctta
     1921 gttctcattc ctggctcctc agctcatcga tcatccgctc taggcggtca atctcatcct
     1981 gcgcgttgcg caattcctcc tgttttccga agcgtaccgt ccagagcagg aacgaggctc
     2041 cgctacccgc cagcccgaat accaggtaat tctgatcgag gacgagtcgt tgcctgagca
     2101 gcaaggcttc ggccggggtg gcggcgactt ggtactcggg gtaggcgctc gtgccgtcca
     2161 cgataccgta gaccgctacg cctaagccgg ctaaacctat aaccgcgcca aggttagaaa
     2221 tcgccgccct ggacttcctg gctttctgga tggctacgag gcgatcgaac gccaggctac
     2281 gttggattgt taaatccgat atcttccatg agttcgagaa gtccagatgc ggtatcgata
     2341 gcgattgctc gctcccgggt acgatttcga tttcctgctt gaacatccac tcccagtcat
     2401 tgccgagctt tgcctctagg acgtatcggc cggctgagaa tagattggtt tgctccaagc
     2461 ttatcggaag gctatcatcc tctccctctt ttgttatccg cgctacgact tcgtcgctgg
     2521 atccccatag gtacttgacc ctgagcccgc cgaagcccga gccgcccgag tagcctcacg
     2581 agccggaggc ggccgttccc gacggggtcg cctgcccgcc cgacgagccg ggccgcatcg
     2641 tcatgctggc tgtcacgcgg accgtggcgc cgtgggccac gctggcggtc gagcgctgct
     2701 ggtcccaatt gtcgcccgtg aaggacaccg agtgcgagcc ttccggaaag aggcccaggt
     2761 cgtactcgtc gcggaggccg tcgcccctgg cgacgacctt gccgtcgacg gctatctgca
     2821 cgttcctcgg cacggacagg gcgacgatgc gcccgccctt gaaggctgcc gtggccgtgg
     2881 acctggcggc cgcgtcgacg gcgaccaggt acggagaggc ccacgattcg tggtacacgc
     2941 tgaagctgcg cggccccggg gccaggccgc ccgcggtgaa gcgcccggcc gctatcgtcc
     3001 cgagctcgcg gcccgcggag tagatcaccg cgccgtccgg gaagccctcg aacgccacgg
     3061 acgcgaagct ctcctgccgg tacgcgaagg acacagcgtt ggccgtatcc gggtagacga
     3121 ccagcttgcg cgtctcctgg ttatcggagt actgcagggt gacgacgcgc tcgccctcgg
     3181 cgacgccggg cagcacgagt gtcgagccgg cggctatcga gccgaccggc ttgccgtcga
     3241 ccagcacggt cgcctcggcc tgggcgctga cggtcaggct gccgaagcgc ggcgcccggt
     3301 agtcgaagcc ctcgctcgag gtctcgccgg agcgcacgag tatcgaacgc cgctcctccg
     3361 agtcgccgta gatcacgcgt agctcgtgga tgccggcgct caggtccttt aggaggagcg
     3421 gcttgcccgg ttccgcggcg ccgaggcccc tgccgtcgac ctcgacgttg gcgctggtgg
     3481 cgacggcgac cgacacggag ccgtagctcg gccgtaggaa gaacgcgaag ctggcgccgg
     3541 cctcgttgcc ggggatggtg cccaggagcg gcacggtctt cgtcacgttg gacctgatgc
     3601 ggtcgtagag gctatgcggg tcgagtatcg ggtcggcgtt gcgctcgagc gtggtgacga
     3661 agtagcggcc gaactcgctc tcgtccggta cggtctccga ggcgccggag gtgagtacct
     3721 gcctggcctg cagcttgagg gccttggcga agtaggcgct gtctatcgtc ggcccggccc
     3781 cgcgcgacac gttgaggaag tcgcccgaga agcacgcgtc ggcgacgacg aggacgcgct
     3841 gggccttgag cagggacagg tagttgcgta tctgctggtt cggtatccag ccccgctgat
     3901 cgaacacgtc cttgacgccg tccgaggcta tccagaagcc ggtgttcgac gagtcgaggt
     3961 agccgtgccc tgcgtagaag atgaacagcg agtcttcctt ccggatcttg cgcggcaggg
     4021 tttcggcgaa cagcttgcga ataccggccg ccgtcgcggc gccgtcatac agctcgatga
     4081 actcgtcgat gtagtagcgc gactcgagga tggccttgac gttcctggcc tcggagaccg
     4141 cgttgcgtag cgagttccat tcctggtagc ggtcgatgcc gatgatcacg gcgtactggc
     4201 ggccgatgtc gaaggacggg tcgccggtta cctggcgtgc ctggacggcc aggccgcgat
     4261 cctgagcgac gagtagctgg gcggcgatga gcatgaccgg aagcatgacc gataggcggc
     4321 gggagactga cggtccgatc aaagcagggt tgcgcatggg aacccccact tttatcagag
     4381 tcatactata gcccctttat agactcttat aaaggaaaat gtaaaaaaaa gcggccggga
     4441 tgagtcccgg ccgcctcgtc gatctctatc gccggatact accggcccga gcgactactc
     4501 gccctctttc acgatctcga tcttgatgcc ctcgtttatc tcgtcggact ctttcggggg
     4561 cacggtgacg cgtaggatgc cgttcttgaa cacggccttg accatggcct ggtcgaactt
     4621 ctccgcgggc acatagtact tctgcttctc gatgtccttg agcttgagcc ggcgcttgaa
     4681 gtagcgcacg ccctcctcgg gggcggcttc gagccctatg cgtgcggaga agaccatgta
     4741 gtcgccctgg aacgataggc tgatgtcgtt ctcgtcgaag ccggacaacg cgaattcgaa
     4801 gaccatcgaa cgatcctggg tgaggaagat gttcatcggc gggtaggaga agctcgggta
     4861 ccagtcggag ttctcgtcct gatccatcat gccgaaccag gggttggagc gctggccgcc
     4921 gggaccggtg gcgcatccgg gccctcccgg gccgaagttg tccttcatct ggtcgccgaa
     4981 attcttggcg gcgtcgaaga tctcgtcgag catcgacccg atgtcgacgt acatgcgatt
     5041 gcctttcata agtatccccc taaggcggta tcgtctgccc ctccgtacgg agcgggcgtt
     5101 gcggacccga gggcgtccgc gggccggtgc ggctatcgcc gtgccggtat acgtacatga
     5161 tacgaacgct ttccttaaaa ggcaagcccg gatcccatgg cgggatccgg gcttgcctgc
     5221 cgacggggct acagcccgcc ggccgttcgg gagaccgtta cttcttgggc gggtacatca
     5281 gcgggtagga gttggccttg ccggcctcgg cccagtcctc ggggatgacc ttccagttgt
     5341 cgtcacgggt gaccgtgatg gggccggtct gcttctcgaa ccacttgagc atatagaagc
     5401 gcaggtcctt cgtggtgctc gaggtgacca gttccagctt ctggagcttc tcggcaggga
     5461 ggccgacgcc cttggtcagg tggccgccgc cgcccgagcc gcggtacgag ttgatggcga
     5521 ccgagtaggt cctaccgagc tcgaagggcg agccgtcggc catcgagatg accttgatcc
     5581 gggaaccggc gggcttggac acgtcgaccg tgtacttgat gccggcgacg gcgtcgtagt
     5641 tgtagggccg ggtgatcgtg tcgtagttgt tgtaacgggc gttatagacc ggcttgcctt
     5701 ccttgtcctt gacgaaggcg atgagctggt cctgcggtcc ggtcatggtg ttgaactgat
     5761 tgccgtagtt gaattccatg aacttgacga tctgctcgcc ggtcaggtcc atcgtgtaca
     5821 tgaagttctc gtacttgtac aggctgaaca tgtcgcgcac cttgatggtg ccatcctgca
     5881 tggtcggcag ataggcgtac tggtcgaggg gggcggctat cgatatctgg gccggcttga
     5941 gacccatggt cgcgtcggcg catagctcga gctgaatgcg gttgatcagg tcgacgaagg
     6001 ccgagtcgcc gaacatcgcc tcgtcgctgt tgatcgagcc gaccatcttg ccgatcgggc
     6061 gctcgaccca gtccatcagc tcggccttgg cgggctcgaa cttggcgagg aacgccgggt
     6121 cggcggccac gcctttgagc ggcacgagcg agccggcgat gtccttcttc caggtcttgg
     6181 cttccttgtc ccaggtcatg gtcacgtcga cgacggccac gttctgggcg ccggagaggg
     6241 cgccgtagat cggcacgacc ttgccggtcg ggtccatgac ggcgacgtcc ttggccttgg
     6301 tctcggggtt gtagcccttg ccttcccagc cggcgtggtc gtggccgacg aagacgaggt
     6361 cgaagcccgc gacctgctcg atgatgagct ggctggcgct ctcgttcttc ggggtcgcct
     6421 tggtctggcc gccgtaggtg tagtcgacgc cggcgtggaa gaggccgatg agcaggtccg
     6481 gcttctcctt ctcgaggatg atcggtaccc agcgcttggc ggtctcgacc atgtcgagca
     6541 cttccatgcc ttcccagaag gccttgggca gctgctcggt gatcttgggc tctatcatgc
     6601 cgagaacggc gatcttgacg ccctggcggg tgatgatcgc gtacggcggg aagtacggct
     6661 cgtccgtacc ttctttgttg atgttggcgc agatcatcgg cgccttgact tccttggcga
     6721 gcttgtcgta gacggcgtgg ccggcctcga tgtcgtggtt gcctatcgtc gccacgtcgt
     6781 actggaggta gttaagcacc tcgctccaga tgtgcgggac gtcggtcttc tcgaagttgt
     6841 agtagtagac ggtcggctgg ccctggagca cgtcgccgtc gtccatcagc acgatctcgc
     6901 cgccctcggc ccgcttctgg cccacgagcg tggcgacctg cgccatggac gtgtccatcg
     6961 ccttgccggt gatgaagttg tacgggaaga tcgccccgtg gatgtcggtg gtctcgataa
     7021 aggtgatctt gagcggcgtt tcggcgaact gctcgaacgg gtgggcgcac gagccgaagg
     7081 ccaaaacgat cgcgagcgcg gccagggccg cgaggatcgt ccttgctgac tttcgcatag
     7141 attcctcctt ggacattcgt cttccgcgtt cgcggaaggg ataacaagta tatacccgag
     7201 cgggttaata tcaaagcgaa aatgcgttcg tgttccgctt aatcggttca gttcgctctc
     7261 ttatttgttg acggacttgc ggagcggtgc gattatcgac cgggcaattt tttttaaggt
     7321 acggtacgag gattcggagg atcgcgaaca tgggaggacg cctggaccgc ttcttgagcg
     7381 cctatgacca ggaaggtctg cagctacgca agaaggcgcg catggttgtg atgacctgcg
     7441 tggctggtag cgtcgcggcg gcgctgatga cggcgacgag ggccgtcctg gccttcgata
     7501 tcgtcagcct cgcgatgggc ctggcgatga tcgccagcgc catcggttgc gccctgttag
     7561 tccggtccgg gcgctatacg ttcggcgtca acttcatcat gggcatgttc tcggtcggct
     7621 tcttcgccct gtcggccgta cggccggccg agaacgcgtc tttcctgtac ctgagcgcgg
     7681 tctacgatgc cttcctgcta tccgcactct gcctgatagc gatacgaacc taccaggtcg
     7741 ccatgttcac cgtcgggtac gtcgcttcgc tcgtcaccct gtttctgttg aagcgtgcct
     7801 ccatcgtcga tccggcggcg ctcgcgccgg tggacctggt ccaggccgtc gtcttcacgg
     7861 cgctgactgg taccgtcggc ttcctggtca tgcgcatgac caacggcacg atagtaatcg
     7921 cggaggccga ggccgcggcc aatgaggacc gcgccgaggg catcaaggct gtcgtcgcga
     7981 acctccaccg aggcctggac tcgggcaacg acctcctggc cgcctcgagc tcggcgctgg
     8041 cgtcgctcca ggccgtggag ctcgcggtag cggcatacga gccgaggctt ccggtcagct
     8101 cgacgcgacg gataaagccg ggcggttgag cgccgagacg accgggaagg tcgccgccgc
     8161 caaatcggat ctggccgccc acgacggcgc ggtgcgcagc gccgtcgcct cggtggccgc
     8221 tatcggtgag tccatcggcc gactctcgac cctggccgcg cgcggcaggg acgctatgac
     8281 gagcctggtc ggcgccgcgt ccgagggcga gcgcagcatc gacgacgcca atgcggccct
     8341 agcggccgtc gagaaggcct cgggagagat gctcggcatc gtcaacgtga tcgccggcat
     8401 cgccagccag accaatctgc tcgcgatgaa cgccgcgatc gaggccgctc acgcgggcgc
     8461 cgccggcaag ggcttcgccg tcgtggccga cgagatccgt aagcttgccg aggagacgga
     8521 ccagaactcc cgctcgatcg ccgatagcct caagcgcaac tcagcaggcg tcgcctcgtc
     8581 gatcaagacg agccgcctgg cccgcgcgtc cttcggcagg atcgcggctg aggtgggaga
     8641 ggcgaatggc tcgatgggcg gcatcctcga ggggctcgac ggcgtgcggg ccgaggcttc
     8701 gtcgctcgac ggcgacctga ggcgtgtctc cgaggcgtcg cgggcggtgg ccgcctcgat
     8761 ggccggcgtc gaggccatgg ccgccgagtc gggctcgggc ctttcggcca tcttcaaggg
     8821 cgccgagtct atcgcctcca aggctgcgga gatcgcctcc gagtacgggc gcatagccgc
     8881 ggagatggag aaagtcgacg gcatcggcaa gg
//
LOCUS       DAOPHV010000032         8777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_10031_length_8777_cov_4.583811, whole genome shotgun sequence.
ACCESSION   DAOPHV010000032 DAOPHV010000000
VERSION     DAOPHV010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8777
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10031_length_8777_cov_4.583811"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1007
                     /locus_tag="PLQ29_01645"
     CDS             183..1007
                     /locus_tag="PLQ29_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HPG85375.1"
                     /translation="MDSPLESGCDLRIGTSGYDYPDWEGELYRHGLGRSEYLGAYSEA
                     FDTLELDYPLRGGAGSGSAPPLSPAEAVSRARRPMDVTLRAAALPGSASPAAILAAVR
                     ELAAAAAPLAEAGTLCALLLPFSFSFRYGDDERRALDRALRELAAFPVAVELLNAEWL
                     NARAIDGLKARRVCLCAVDLPRIDGLPPVSDLVTSDLACVRFHGRDGEAWLAGDRGSR
                     AGYRYSRDELGSWLPRLEAMSVEARKLRVYFVNRRGGAAPAGARELLSLARSFRLL"
     gene            1018..2211
                     /locus_tag="PLQ29_01650"
     CDS             1018..2211
                     /locus_tag="PLQ29_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85376.1"
                     /translation="MPSGRVLHINAVGLMAELEESLEPSLRRRPFVVANERLARAVVL
                     DVSALAHREGARPGMSLAAARALLPGLAARPPRSELYRDAEDRLWRLALELTPLVERA
                     GRGHLFADLTGTGRLNGSPDDAAQRLRSRILADTGLSPALALSSTKTASKVATRVFRP
                     GGFVALSTAEEGPLVRRQPASLLPGVGPALLRRLALLDIDDIGGIADLDDTRAAAVGP
                     RGRELVARARCVDDSPVDPRPLGSRSASAEAVLEPDTADPETLRLRLLSLSAELAFSL
                     RREGLGFSGASVSLGYTDGLRASASACSGRRLWRDDEALALALEALGRARTRRVRVRS
                     VALTLSRLGAASPELDLFEPGESRRSRLQSALDAIRLRYGSGAIEPCALAELGAPGPS
                     GAIRW"
     gene            2205..5198
                     /gene="dnaE"
                     /locus_tag="PLQ29_01655"
     CDS             2205..5198
                     /gene="dnaE"
                     /locus_tag="PLQ29_01655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPG85377.1"
                     /translation="MVAPLLVASGYSFHYGCATPAELAGRAKALGYEALALADRDGLY
                     GVPAFIESCEAEGLKPLIGAELSLDGGGPGSGRALAIAMDRAGFSRLCRLLSARAEPG
                     FDARAELLRDSVGLAVASDDDRLLGSPGSDGFYALLSPCRRGSWRRLLASGRRPLATG
                     EVRFIEPGDRALQRLLVAIGSGRTVYEVRDDELSPEGATLVDPAGFGAAYAGAPEALA
                     ANEALVGAVSFRSPFDGFVFPRHGGPGEEKGYDPVAALRRLTLEGAVGRYGVGPHGTL
                     PGRVTARIDYELEIIAEKGFADYFLVVRDIVRRASRSCGRGSAAASVVSYALGITDVD
                     PIRHGLYFERFLNPGRADPPDIDVDFAWDERDGLLESVLAEYGEARAARVSNHVRFQA
                     RSALREAARAYGMPDGEISARERSAASADPGGAGRDAPRDGEWAEIARLAARLPGLPR
                     HLGVHSGGVILVPDELASRVPVERSGSGIRVTAWDKDGVEAAGLVKIDLLGNRSLAVV
                     RDALVNLEENGEPIDERAWRPIDDPGTIAMLARGDAMGVFYVESPAMRLLQKKTGRGD
                     FEHLVIHSSIIRPAANRYISEYIDRLNGKPYLPLHPLIGDLLAESYGIMCYQEDVCKV
                     AVALAGFSPAEADKVRKALSKKDAPERLAAYAGRFRDGALARGVDERTIAETWAMIES
                     FSGYSFVKAHSASYAMLSFRSAYLRRHHPAEFMAAVISNRGGYYSTLAYVSEARRMGL
                     RVLPPDVETSRLRCRGRGSELRLGLGMVSTLSSACSEAIVRARRAGGPFRSVDALARR
                     ARVGRPDAEALAGSGALDSLSPGMTRADKLARLLSLCDEGKREAGQGELFAYEPAVPG
                     PARPPGSSGPSGPSGPGARKRLESELLYLGTTLAEHPLALWPKALSMPRALGRDLPRL
                     VGRRVALIGWPITAKPVVTSGGEAMEFVSFEDETALYETVLFPGAFARYRHLLFDERP
                     LIVRGVVEEDRTAVTVTVDSLERLG"
     gene            complement(5221..6333)
                     /locus_tag="PLQ29_01660"
     CDS             complement(5221..6333)
                     /locus_tag="PLQ29_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPG85378.1"
                     /translation="MKEKARTTILIVENDVLSALNVRETLEAAGYATLSTGTGEDAVR
                     LASDSSVGLVLMDISLDGELDGIDAAEAILRASSRPIVFMSSHDDAATMIRLERVDAY
                     GFMPKPFGNASLLATVSMALRRSAAERSAADRMVADYERRLLQSDTRLKESHHRIRND
                     IAAISSILSLQLGEAEHPETVEALSDALGRVESLSTLYEMLTVHDSGGDIEVRPYLER
                     LIGAIMPIFPGGSGIDVDCRVDEFMLDQKRTFTLGIIVNELLTNALKYAFDGRSEGSI
                     RLDVCVYDESVIMTMRDDGKGLPERFDIAESSGLGLTLISMLLDQFDGTLTMRSDHGT
                     VSTALIKAPGLEATAPAAPAGLEAAIVAKRARVNVA"
     gene            complement(6624..7589)
                     /locus_tag="PLQ29_01665"
     CDS             complement(6624..7589)
                     /locus_tag="PLQ29_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPG85379.1"
                     /translation="MRLGIAGSGMIVEDGLRAISEAKGVTAVAICAREKSRDRAEALA
                     ARHGVGSTVYDYDELLGRADVDAVYIALVNDLHYDYAKRALEAGKHAIVEKPFTVTLA
                     EAEALVALARSRGLFLFEAAPTVHMPLFRSIVERAAGIGRIGLVQCSYCQRSSRYDRY
                     LAGDVAPAFDPAHAGGALRDLNVYNLHLAVRLLGLPNTASYSARRGWNGVDTSGSALL
                     GYGGASATCVAAKDADGPGFSLVIGESGWIRADGSPNALSRVEYGAPGLGIAELRDDS
                     GLPRLAHEWIAFERMMREGRLDECYDLLEHSLRVMKVLDSLSRGL"
     gene            complement(7606..8055)
                     /locus_tag="PLQ29_01670"
     CDS             complement(7606..8055)
                     /locus_tag="PLQ29_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPG85380.1"
                     /translation="MIPDKVLSALDAHGLSALEFEPGSTPTVELAAARIGVEPARIAK
                     SLLFKSKDERYVMLVCPGDKRVQSGALKRLAGSKLSMTDAEETERVTGYKPGGVCPFA
                     LTGVEVLLDRELGDYETVYPAAGTDATGVPASLAQLEAATGGRIVDL"
     gene            complement(8052..>8777)
                     /locus_tag="PLQ29_01675"
     CDS             complement(8052..>8777)
                     /locus_tag="PLQ29_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG85381.1"
                     /translation="ARGRGIGTALLSWLEGEAAALAQRDHPGEPSEFAGNASSTETSA
                     TKLLTDAGYRAAYTVAELRLDSSSHAEARAPALPDGLVIVAPGKDDYRKLTGTVVAAF
                     AKEYSEGRFDEPVDPGEYEESLSDDDREPSLWRIVKRGDEIVAVALCDARTDAMAEVV
                     ELAVSPDWRRRGIARALMTATIDAAIAAGFRELRVYTIEQFQTRAIQLYQSLGFARYK
                     DFPRYRKPMPGLPPRTLDARIGA"
BASE COUNT         1254 a         3039 c         3035 g         1449 t
ORIGIN      
        1 cttgccggag tccggatgcg atatgatcgc gaaggtaagc cgtctgcgta gctgggtatc
       61 tagttcgctc atttgtctgt ggtcaaacca taccaagaga ccgggccggg tgtatagtgc
      121 cggaggcgga tcgggctcga cctctccttg catactaaac ggatgtatag tacaactgag
      181 acatggattc gcctcttgaa tcgggctgcg acctgcgcat cggaacctcc ggatacgact
      241 acccagactg ggagggcgag ctgtaccgcc acggcctcgg caggtcggag tacctgggcg
      301 cgtacagcga ggccttcgat acgctcgagc tcgactatcc gcttcggggc ggcgcggggt
      361 ccggttccgc gccgcccctc tcgcccgccg aggccgtatc ccgcgccagg cgcccgatgg
      421 acgttaccct gcgggcggcg gcgctacccg gctccgccag ccccgccgcg atcctggccg
      481 cggtccggga gctggcggcc gccgccgctc ccctcgccga ggccgggacg ctgtgcgcgc
      541 tcctcctgcc gttctcgttc tcgttccgct acggcgacga cgagaggcgc gcgctcgacc
      601 gggcgctccg ggagctggcg gccttcccgg tcgccgtcga actgctcaac gccgagtggc
      661 tcaacgccag ggccatcgac gggctcaagg ccaggcgggt ctgcctgtgc gccgtcgacc
      721 tgccgcggat cgacggcctg ccgccggtgt ccgacctggt aacctccgac ttggcctgcg
      781 tccgcttcca cgggcgagac ggcgaggcct ggctggccgg cgaccggggc tcccgggccg
      841 gctaccgcta tagccgcgac gagctcggct cctggctgcc caggctcgag gctatgagcg
      901 tcgaggcgcg gaagttgcgc gtctatttcg tcaaccgccg cgggggcgcc gccccggcgg
      961 gcgcccgcga gctcctgtcg ctggcgcgct ccttccgcct gctgtgagct gtcggccatg
     1021 ccgtcgggtc gcgtcctgca tatcaacgcc gtcgggctga tggccgagct cgaggagtcg
     1081 ctcgagccgt ccctccgccg ccgccccttc gtcgtggcca acgagcgcct ggcgcgggcg
     1141 gtcgtgctcg acgtgtccgc cctggcgcac cgggagggcg ctcggcccgg catgagcctg
     1201 gccgcggccc gcgcgctcct gcccggcctg gccgcgaggc cgccgcgctc cgagctgtac
     1261 cgggacgcgg aggaccggct ctggcgcctg gcgctcgagc taacgccgct cgtcgagcgg
     1321 gcggggaggg gacacctctt cgccgacctg acgggcacgg gccgcctgaa tggctcgccg
     1381 gacgacgccg cgcagcggct gcgctcgcgc atactcgccg acaccggcct gagcccggcc
     1441 ctggcgctgt cgtccaccaa gacggcgagc aaggtggcga cccgggtctt caggcccggc
     1501 ggcttcgtcg ccctgtcgac cgcggaggaa gggcccctcg tcaggcgcca gccggcgtcc
     1561 ctgctgccgg gcgtcggtcc ggccctcctg cgcaggctgg cgctgctcga catcgacgac
     1621 ataggcggta tcgccgacct ggacgacacg cgggcggccg ccgtcggccc gaggggccgg
     1681 gaactcgtgg cccgcgcccg ttgcgtcgac gactcgcccg tcgacccgcg gcccctcggg
     1741 agccggagcg cgtcggccga ggccgtgctc gagcccgaca cggccgaccc ggaaacgctg
     1801 aggctcaggc tcctgtccct gtcggcggag ctcgctttct cgctccggcg cgaggggctc
     1861 gggttctcgg gcgcctcggt gtcgctcggg tataccgacg ggcttcgcgc ttcggcctcg
     1921 gcctgctcgg gccgccggct gtggcgggac gatgaggccc tcgccctggc cctcgaagcg
     1981 ctcggccgcg ccaggaccag gagggttcgc gtacgctcgg tcgcgctgac gctatctcgc
     2041 ctcggcgccg ctagcccgga gctggacctg ttcgagccgg gcgagtcgcg ccgctcgagg
     2101 ctgcaatcgg cgctcgacgc gatacgcctt cgttacggtt ccggcgccat cgagccctgc
     2161 gccctggccg agctcggcgc gccaggcccg tccggggcga tccgatggta gcgccgctcc
     2221 tggtcgcctc gggctattcc ttccattatg gctgcgcgac gcccgcggag ctggccgggc
     2281 gcgccaaggc cctcggttac gaggcgctcg ctcttgctga ccgcgacggg ctctacggcg
     2341 taccggcctt catcgaatcc tgcgaggccg agggcctcaa gccgctcatc ggcgcggagc
     2401 tgtcgctcga cggcggtggt cccggaagcg gccgggccct cgcgatagcg atggaccgag
     2461 cgggcttctc gcgtctctgc cgcctcctca gcgcccgggc cgaacccggc ttcgacgctc
     2521 gcgcggaatt gctccgcgac tccgtcggcc tcgcggtggc ctccgacgac gatcggctcc
     2581 tcggctcgcc aggctcggac ggcttctacg cgctcctgtc gccgtgccgg cgcggctcct
     2641 ggcgccgcct tctcgcctcc ggccggcggc cgctcgcgac cggagaggtc cgcttcatcg
     2701 agccggggga ccgggccctc cagcgcctgc tcgtcgccat aggctccggg cgaaccgtct
     2761 acgaggtccg cgacgacgag ttgtcgcccg agggcgctac cctcgtcgat ccggccgggt
     2821 tcggcgccgc gtacgccgga gcacccgaag cgcttgccgc caacgaggcg ctcgtcggcg
     2881 ccgtctcgtt ccgctcgccg ttcgacggct tcgtcttccc gcgccacggc gggccgggcg
     2941 aggagaaggg ctacgatccg gtagcggcgc tgcgccgcct gacgctcgag ggcgccgtcg
     3001 gacgctatgg cgtcggcccc cacggaaccc tgcccgggcg cgtaacggcc cggatagatt
     3061 acgagctgga gatcatcgcc gagaagggct tcgcggacta tttcctcgtg gtccgcgaca
     3121 tcgtgcgacg cgcctcgcgc agctgcggcc gcggcagcgc cgcagccagc gttgtctcgt
     3181 acgctctcgg catcaccgac gtcgatccta tacggcatgg cctgtacttc gagcgcttcc
     3241 tcaaccccgg ccgggccgac cctccggata tcgacgtcga cttcgcctgg gacgagcgcg
     3301 acgggctgct cgagtcggtg ctcgccgagt atggcgaggc cagggccgcc agggtctcga
     3361 accacgtccg cttccaggcc cgctccgccc tgcgcgaggc cgcccgagcc tacggtatgc
     3421 ccgacggcga gatctccgcc cgggagcgga gcgccgccag cgctgaccct ggcggggctg
     3481 ggagggacgc tccacgcgac ggcgaatggg ccgagatcgc ccgtctcgcc gccaggctgc
     3541 cgggcctgcc ccgccacctc ggcgtccatt ccggcggcgt catcctggtc cccgacgagc
     3601 tcgcctcgcg cgtgccggtc gagcgctccg gttcgggcat acgcgtgacc gcctgggata
     3661 aggacggcgt cgaggcggcg ggcctcgtca agatagatct gctcgggaac cgctcgctcg
     3721 ccgtcgtgcg ggacgccctc gtcaacctgg aggagaacgg cgagcctatc gacgagcgcg
     3781 cgtggcggcc catcgacgac ccaggaacga tagccatgct cgcgcgcggc gacgcgatgg
     3841 gcgtcttcta cgtcgagtcg ccggcgatgc gcctcctcca gaagaagacc ggccgcggcg
     3901 acttcgaaca cctggtcatc cattcgtcga tcatacgccc ggcggccaac cgctacatct
     3961 cggagtacat cgaccggctg aacggcaagc cctatctgcc gctgcacccg ctgatcggcg
     4021 acctgctcgc cgagagttac ggcatcatgt gctaccagga ggacgtctgc aaggtcgcgg
     4081 tggccctggc cggcttttca ccggccgagg ccgacaaggt gcgcaaggcg ctgtccaaga
     4141 aggacgctcc ggagcgcctg gcagcctacg ccggccgctt ccgcgacggc gcgctggccc
     4201 gcggggtcga cgagcggacg atagccgaga cctgggcgat gatcgagtcg ttctccggct
     4261 actcgttcgt caaggcgcac tcggccagct acgcgatgct gtcgttccgt tcggcctacc
     4321 tgcgccgaca ccatcccgcg gagttcatgg ccgcggtcat ctcgaaccgc ggcggctact
     4381 actcgacgct ggcctacgtc agcgaggcca ggcgcatggg cctccgcgtg ctgccgcccg
     4441 acgtcgagac gagccgcctc cgctgtcgag gccggggctc ggagctacgg ctcggcctcg
     4501 ggatggtctc gacgctgtcg tccgcgtgct ccgaggccat agtccgcgcg cgacgcgccg
     4561 gtgggccgtt ccgctcggtc gacgccttgg cgcgcagggc ccgcgtcggg agacccgacg
     4621 cggaggcgct agccggctcg ggcgccctcg actcgctgtc gcccggcatg acgcgggcgg
     4681 acaagctcgc gcggctcctg tcgctatgcg acgaaggcaa gcgggaggcc ggccagggcg
     4741 agctgttcgc ctacgaaccc gccgtccccg gtccggcgcg gccgccggga tcgtcggggc
     4801 cgtcggggcc gtcggggccg ggcgcgcgca agcggctcga gtcggagctg ctctacctcg
     4861 gcacgacgct ggcggagcat ccgctggccc tatggcccaa ggcgctctcc atgccgcgcg
     4921 cgctcgggcg cgacctgccg cgcctggtcg gccgccgcgt cgcgctgata ggctggccga
     4981 taacggccaa gcccgtcgtc acctcgggcg gcgaggctat ggagttcgtg tcgttcgagg
     5041 acgagaccgc gctgtacgag acggtgctgt ttcccggagc gttcgcgcgg tatcgtcacc
     5101 tgctcttcga cgaacggccg ctcatcgtcc ggggcgtcgt cgaggaggac cggacggccg
     5161 tgaccgtgac cgtggatagc ctggagcggc taggataggg gagggcggcg ccggaacggc
     5221 tcaggcgacg ttgactcggg cgcgcttcgc gacgatggcc gcctcgaggc cagccggagc
     5281 ggccggcgcg gtggcctcga gcccgggcgc cttgatcagc gcggtgctga cagtcccgtg
     5341 gtcgctgcgc atggtgagcg taccatcgaa ctggtcgagc aacatcgata tcagcgttag
     5401 acccaggccg ctcgactcgg ctatatcgaa gcgctcggga aggcccttgc cgtcgtcccg
     5461 catcgtcatg atgaccgact cgtcgtacac gcagacgtcg aggcgtatgg aaccttcact
     5521 gcggccatcg aaggcatact tgagcgcgtt cgtcaggagt tcattgacga tgatgcccag
     5581 cgtgaaggtc cgtttctggt cgagcatgaa ctcgtcgacg cggcaatcga cgtcgatccc
     5641 ggagccgccg gggaagatcg gcatgatggc gcctatgagg cgctccaggt acgggcgcac
     5701 ctcgatatcg ccgcccgagt cgtgtacggt gagcatctcg tagagcgtgc tcaagctttc
     5761 aacgcggccg agcgcgtcgc tgagggcctc gacggtctcg ggatgctcgg cctcgccgag
     5821 ctgcaaagac aggatggacg agatcgccgc tatatcgtta cggatacggt gatgagactc
     5881 cttgagccgc gtgtcgctct gcaggaggcg ccgctcgtaa tcggcgacca tacgatccgc
     5941 cgccgaacgc tcggccgcgg atcgcctgag cgccatcgat acggtagcca ggagcgacgc
     6001 gttgccgaat ggcttgggca tgaagccgta cgcgtcgacg cgctcgaggc ggatcatcgt
     6061 cgctgcgtcg tcgtgggaag acatgaacac gattggtcgc gaggaggcgc gcaggatcgc
     6121 ctcggccgcg tcgatgccat ccagctcccc atcgaggctg atatccatca ggacgaggcc
     6181 gacggacgag tccgaggcga ggcgcacggc gtcttcgccc gtaccggtgg atagcgtggc
     6241 gtatccagcg gcctcgagcg tctcccgtac gttgagcgcg ctcaggacgt cgttctcgac
     6301 gatgaggatg gtcgtgcgcg ccttctcctt cactccgctt gctccatcgg acggattcga
     6361 cgcatacaac gcgatcggcc tagctaggct acgccgatcg cgttcgtacg cactacgttt
     6421 atataaattc ttatgagatg attttgaaag cgttttcatt acgtggatcg tatctccggg
     6481 tgtagagccg atcaagctgt tttttgcccg aagcgtgtag acaattgtct acaagggtca
     6541 aaatcacctc aaattcgtta gaaatatcaa atattatgcg gtgtcatata ggaataaccc
     6601 tatgtctata gaggttcaac gcgtcacagt ccgcggctca ggctatccag taccttcatg
     6661 acccgcaggc tgtgttcgag taggtcgtag cactcatcga gtcggccttc acgcatcatc
     6721 cgctcgaagg ctatccactc gtgggctagc cgaggtaggc cggagtcgtc gcgtagctcg
     6781 gcgatgccta ggcccggcgc tccgtactcg acgcgggaca gcgcgttcgg cgatccgtcg
     6841 gcccgtatcc atcctgattc gccgatgacg aggctgaagc caggtccgtc cgcgtccttg
     6901 gctgccacgc aggtagcgct cgctccgcca tagccgagca gggccgagcc ggaggtgtcg
     6961 actccgttcc agccgcgcct cgctgagtag ctggccgtat tcggcaggcc aaggaggcga
     7021 accgccagat gtagattgta gacgttgagg tcgcggagcg cgccgccggc atgggccgga
     7081 tcgaaggccg gcgccacgtc gcccgcgagg taacggtcgt agcggctcga gcgctggcag
     7141 taggagcact ggacgaggcc gatgcggccg atcccggcgg cccgctcgac gatggagcgg
     7201 aacagaggca tgtgcacggt gggcgccgcc tcgaagagga agaggccgcg ggaacgggcc
     7261 agggcgacca aggcttcagc ctcggccagg gtaacggtga atggcttctc gacgatggcg
     7321 tgcttaccgg cctcgagagc cctcttggcg tagtcgtagt gcaggtcgtt gaccagagcg
     7381 atgtagaccg cgtccacatc ggcccggccg agtagctcgt cgtagtcgta caccgtcgag
     7441 ccgacgccat gacgggcggc cagggcctcg gccctatcgc ggctcttctc gcgcgcgcag
     7501 atggcgacgg cagttacgcc cttcgcctcg gagatggctc gcaggccatc ctcgacgatc
     7561 atgccgctac cggcgatgcc taacctcata gtcgataccc ccgtctcaga gatcgacgat
     7621 cctgccgccc gtggcggcct cgagttgcgc cagactggcc ggaacgccgg tagcgtcggt
     7681 cccggccgcc ggatagacgg tctcgtagtc gccgagctcg cgatcgagca gtacctcgac
     7741 tccggtcagg gcgaaagggc atacgccgcc tggcttatag ccggtgacgc gctcggtctc
     7801 ctcggcgtcg gtcatcgaca gcttggagcc agcgaggcgc ttgagggccc cggattgtac
     7861 ccgcttatcg cccgggcaga cgagcatgac gtagcgctcg tccttggact tgaacagtag
     7921 ggacttggcg atccgggcgg gctcgacgcc gatgcgcgcc gcggcgagct ccaccgtcgg
     7981 ggtggagccg ggttcgaatt cgagagcgct cagcccgtga gcgtcgagcg cgctgagtac
     8041 cttgtcggga atcatgcacc gattctagcg tccagggttc gcggaggcaa gcctggcatc
     8101 ggcttgcggt atcgggggaa gtccttgtaa cgggcgaagc cgagcgattg gtagagctgg
     8161 atcgccctgg tctggaactg ctcgatcgtg tatacgcgta gctccctgaa tccggccgcg
     8221 atcgcggcat cgatggtcgc cgtcatcaag gcgcgggcta tgcccctgcg cctccagtcc
     8281 ggggaaacgg cgagctcgac gacctcggcc atggcgtcgg ttcgagcgtc gcagagcgcg
     8341 accgccacga tctcgtcgcc tcgcttgacg atccgccaga gcgacggctc tcgatcgtcg
     8401 tccgaaaggc tttcctcgta ttcgcccgga tcgaccggct cgtcgaacct gccttccgag
     8461 tactccttgg cgaaggcggc gacgaccgtt ccggtcagct tccgataatc gtccttcccg
     8521 ggggcgacga tgacgaggcc gtccggcagg gccggagccc gagcctcggc gtggctacta
     8581 gagtcgagcc gtagctccgc cacggtatac gccgcgcgat agccggcatc ggtgaggagc
     8641 ttcgtcgcgc tcgtctcggt cgaagaggcg ttgcctgcga attcgctcgg ttctccgggg
     8701 tgatcgcgct gagcgagcgc cgccgcctcg ccctccagcc atgagaggag ggcggtgccg
     8761 atgcctcgcc ccctggc
//
LOCUS       DAOPHV010000033         8776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_10034_length_8776_cov_4.486412, whole genome shotgun sequence.
ACCESSION   DAOPHV010000033 DAOPHV010000000
VERSION     DAOPHV010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8776
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10034_length_8776_cov_4.486412"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLQ29_01680"
     CDS             <1..530
                     /locus_tag="PLQ29_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85382.1"
                     /translation="RRRLIESGKRLDVPLRLNDDPTETRQLAPGEESRDWVAWPMPTI
                     EPEEMRRLLDFEDDATLASLRGIIVFRIKERALWRAKQALATIVTLGPLVLGALAAIG
                     VSRFGLGTAAWLAIFGAVEAIHLAELPAALRAWKKEAERRNAPWSGARTVRAVAATLS
                     CGYPSWVPRKLGVFD"
     gene            620..1945
                     /locus_tag="PLQ29_01685"
     CDS             620..1945
                     /locus_tag="PLQ29_01685"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG85383.1"
                     /translation="MISPIQHNGIFLGLSSFQALAMFRRGIFYTFLAVYLRSHLGLSV
                     TETTLFETVPMALNVLFQTLVWGRLTDRLQKRRTLIIIGELMASVGHVAMWYFHAVAP
                     NPRAAGYVIIAGLTVIEAFWSMSNIGWSAFISDVYTAEERNAVQGRFQSIGGVGRIVG
                     AMAAGLLYDGIGRAYPGWGYGAGGIFFVSAFVMAGSVVPILFLPEGGIAFRSSGAEKA
                     PKADDDARGGARVGGKAPGRSLAVFATFLVAMFLMNSGINTLGAFRGQFLDLPEGFAA
                     PARTISRVVNVESAALIVIGFFVGSLGKRLGITRLLVAGALSGIVSMTLHAVAPSLGV
                     IYLGALFKGVSDGCIAASSYAFASTLIPPEKRGRYFAFYNSTFFLSWGVTATFLTGPL
                     IDALIGAGRGAVLAYRVGLASGAALMAAGLALLVALLAFMRRRRGNADA"
     gene            complement(1942..2637)
                     /locus_tag="PLQ29_01690"
     CDS             complement(1942..2637)
                     /locus_tag="PLQ29_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85384.1"
                     /translation="MEPNTPPEDQAGGACVELDASALAGLRKMPVDVFDDVYLGEVKP
                     DALVLCVDVRNFSDFLCSHDEDEVFALIKDFTSNLLSCVNQFGYGCSYYKLMGDGAIV
                     IWDTFDEASAREALAVFDSYTDFVDEELFKPYPDLGLAGALVVEKVFKYEISAEASQL
                     KYRDYVGYGINLACRLQALARSGELAVNERLASSGLVTAVPDASSGILAELRSMKGLK
                     DEDRTRALLYRKA"
     gene            2701..4758
                     /locus_tag="PLQ29_01695"
     CDS             2701..4758
                     /locus_tag="PLQ29_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG85385.1"
                     /translation="MSNKRLALRVTRNSVVMIIAVYLVMQVFTYFRDGLILGVSGLSD
                     LLAMTAAYMFSVVIPPALVLGLILYLLALRIQRVACALERGETIPDDELERTRKRLIG
                     FSKVVLAENIVGFASGYVIFVVSQGFLHEILRFDRLVVLVSNLAGGVIYASAQNALNE
                     IVFADIRDRLGIREIGERRRERSTTGKQIGLSAALVVYAVTFIQFNVKDTVAFNKVGD
                     DILAGVVSGSILPEDAGTAYRSLLAERLPELGTRSSVGADDVPLPWERADSAVRRQQT
                     MFIMYAAFTLCICCAVQAIVSSSLRRQLQAIANRVKDVLDGGGDLRIRLSIRAMDDLG
                     ELTELLNRLLDRFHSIARDIGHTARRTRESSEAIDRELIRSESLARGTRETVRELETT
                     LQSQAAATRTFVDALRSFRDGVAAADAATEDQKRFVAETSAAMEEMAASIRSISSMTE
                     RAGSLSGRLSESGDAGSAAVRDTRAAIDEINAAAREVLGVLTTLNKISADINLLAMNA
                     AIEAAHAGEHGQGFAVVADEVRTLAGNAAQRTRAIKELIQAMGARVDNGVRQAEASGA
                     ALAGLTAGLGESASISREIAAAAKEQAAGTDAVIGSITQAVRSADTVSAMMGDQARRS
                     DEIAMALEGVLRRLEGLAADASRQTREVTALEESFAAVRKEVDGNLESVGALGSEMAR
                     FSV"
     gene            complement(4772..5500)
                     /locus_tag="PLQ29_01700"
     CDS             complement(4772..5500)
                     /locus_tag="PLQ29_01700"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPG85386.1"
                     /translation="MTVTELLEAISPPRPLPGAAARTVMAPPFRRQELEALACLGDPK
                     PAVPSGAGWREAAVLVLFYESGGGLRFPLIVRPDGPGVHAGQVSLPGGAREPEESPED
                     CALRETREEIGVDPGSVRLVRALTPLHVPPSRFLVRPFAGVAAARPEFRLSGSEVAVL
                     LEPRLDELLDPAGASCVDVRLGVAAGVGDGIADGIADGVAVGVAVGVAEAPCYELAGY
                     SVWGATAMILSELRAMIVGELAAG"
     gene            complement(5652..6296)
                     /locus_tag="PLQ29_01705"
     CDS             complement(5652..6296)
                     /locus_tag="PLQ29_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPG85387.1"
                     /translation="MTVGNRMSRHLVTVGADTAVPEAEALMRREKIHRLPVVGKKGEL
                     LGIVTASDLRRASAPPATSLDIYELHYLISRLKVESVMTKDPVTVSEDLPVEEAARIM
                     DDNRIAGLPVTRGGELVGIITESDLFRLFIDLFGARHRGVRLTLLMPEKRGELAAIAS
                     AIAEAGGNLLSLVAFDGDDGKTGYCTLKVEGVAKDALVSVVEPLVERVMEAREG"
     gene            complement(6313..7020)
                     /locus_tag="PLQ29_01710"
     CDS             complement(6313..7020)
                     /locus_tag="PLQ29_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG85388.1"
                     /translation="MMELRDVCTYYGNIQALRGISLSVEEGEIVTLIGANGAGKTTTL
                     MSICGIAQIRSGQVLLDGEDISRKSPHSIVAMGLSQVPEGRRIFPQLTVLENLDMGAF
                     LRRDKATVRVDLEEVFDLFPRLAERRNQQGGTLSGGEQQMLAISRALMARPRILLLDE
                     PSLGLAPLIVQNIFDIIKRINEERRTTVFLVEQNANMALKIADKGYVLQNGSIKMADS
                     AERLLANEEVRKAYLGL"
     gene            complement(7028..7813)
                     /locus_tag="PLQ29_01715"
     CDS             complement(7028..7813)
                     /locus_tag="PLQ29_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG85389.1"
                     /translation="MSVANPILEIDGLSMVFGGLRAIDDVSLHIREGEICALIGPNGA
                     GKTTIFNCITGVYKPTEGRVTIRRGGGQREAIQGLKPNVINRKGLARTFQNIRLFGNM
                     TVLENVMIGRHNALAAGVIRAIARDAATRDEEARLIDDCRRILVKMKLDPFINETARN
                     LPYGAQRRLEIARALATDPFLLLLDEPVAGMNPHETKELEETIDLIRDEEKVTILLIE
                     HDMSLVMNVSERIYVLDYGRLIAEGTPEEIKGNPEVIRAYLGE"
     gene            complement(7810..>8776)
                     /locus_tag="PLQ29_01720"
     CDS             complement(7810..>8776)
                     /locus_tag="PLQ29_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPG85390.1"
                     /translation="ALGMYHTNIMITALIYVILALGLNIVVGLGGLLNLGYAAFFAVG
                     AYTYGLLWKYADPAFVAAGLDPGWLFWIALPTAGVLAAIFGALLSLPVLRLRGDYLAI
                     ITLAFGEIVRMVLQNSGEVTGGATGISLIPRPWFFGLRLPPRQAATYIYYIAVALVAL
                     TVFVVRRIEDSRVGRALEAMREDEIACEAMGVDLVRNKLITFSLGAFWAGIAGVLLAA
                     QTTYINPDSFTLWESITVLMAVVIGGTGSIPGAIGGALLLKLLPEYFRPLAQYRMLIY
                     GIAMILVIIFKPDGLLPRNRKKYTFDGSPSAVGAPVAAGDQEARR"
BASE COUNT         1467 a         2966 c         2885 g         1458 t
ORIGIN      
        1 cccggaggcg cctgatcgag tcgggcaagc gcctcgacgt gccgctccgg ctgaacgacg
       61 accctaccga gaccaggcag ctcgcccccg gcgaagagag ccgcgactgg gtcgcctggc
      121 cgatgccgac tatcgagccc gaggagatgc gcaggctcct cgacttcgag gacgacgcga
      181 cgctggcctc gctacgcggt atcatcgtct tcaggatcaa ggaacgcgcg ctctggcgcg
      241 ccaagcaggc cctggccacg atcgtgacgc tcgggccgct cgtcctcggg gcactggccg
      301 ccatcggagt ttccaggttc gggctcggca cggcggcctg gttggccatc ttcggtgcgg
      361 tggaggcgat acacctggcc gagctgcccg ccgcgctcag agcctggaag aaagaggccg
      421 agcgccgcaa cgcgccgtgg tcgggggcaa ggaccgttcg cgccgtggcc gccaccctga
      481 gctgcggcta cccgagctgg gtaccgagga agctgggcgt cttcgattaa cccggagccg
      541 cgcggcttga ccttcagagc gctttactag aagcttcctt cggagccgcc gatccggccc
      601 cgaaggaacc gctcatacca tgatatctcc gatccagcac aacggcatct tcctcggtct
      661 ctcctcgttc caggctctgg ccatgttcag gcggggcatc ttctacacct tcctagcggt
      721 ctacctgcgc tcccacctgg gtctgtcggt taccgagacc acgctgttcg agaccgtacc
      781 gatggcgctc aacgtgctgt tccagaccct cgtctggggt cgcctgaccg accggcttca
      841 gaaacgtcgg accttgatca tcatcggcga gctcatggcc agcgtcggcc atgtggccat
      901 gtggtacttc cacgccgtcg cgcctaaccc gcgcgcggcc ggctacgtga tcatagcggg
      961 actgacggtg atcgaggcct tctggtcgat gtcgaacata ggctggagcg ccttcatctc
     1021 ggacgtctac accgccgagg aacgcaacgc ggtacagggc aggttccaga gcataggcgg
     1081 cgtcggcagg atagtcggcg cgatggccgc cgggctcctg tacgacggga tcggccgcgc
     1141 ctatcccggc tggggctacg gggcaggcgg tattttcttc gtctccgcct tcgtgatggc
     1201 cggctcggtc gtgccgatac tgttcctgcc cgagggcggc atcgcgttcc gctcgagcgg
     1261 agcggagaaa gcgccgaagg ccgacgacga cgcgcgcggc ggcgccaggg tgggaggcaa
     1321 ggcgcccggt cgaagcctgg ccgtcttcgc gaccttcctc gtcgccatgt tcctcatgaa
     1381 ctcgggcatc aatacgctcg gcgccttccg cggccagttc ctggatctgc ccgagggctt
     1441 cgccgcgccg gcgaggacca tcagccgcgt cgtcaacgtc gagtcggccg ccctgatagt
     1501 gatcggcttc ttcgtcggct cgctcggcaa gcgcctgggc ataacgcggc tattggtagc
     1561 cggggcgctg tcgggcatcg tctcgatgac gctccacgcc gtcgcgccga gcctcggcgt
     1621 catctacctg ggcgcgctgt tcaagggcgt gtccgacggc tgcatcgcgg cctcgtccta
     1681 cgccttcgcc tcgacgctga taccgccgga gaaacgaggg cggtacttcg cgttctacaa
     1741 tagtaccttc ttcctgagct ggggcgttac ggccaccttc ctgaccggtc cgctgatcga
     1801 cgcgctgatc ggagccggta ggggtgccgt gctggcctac agggtcggcc tggcctcggg
     1861 ggcggcgctg atggcggccg ggctggccct gctcgtcgcg ctgctcgcct tcatgcgacg
     1921 acggcgcggg aacgcggacg cctaggcctt gcggtacagc agggcgcggg tcctgtcctc
     1981 gtccttgagg cccttcatgg agcgcagctc ggccaggata cccgacgagg cgtcggggac
     2041 cgccgtaacg agcccgctcg aggcgaggcg ctcgttgacg gctagctcgc cgctgcgcgc
     2101 cagggcctgg aggcgacagg ccaggttgat gccgtaaccc acgtagtccc ggtacttgag
     2161 ctgcgaggcc tcggcggaga tctcgtactt gaagaccttc tcgacgacga gggccccggc
     2221 cagtcccagg tcgggatagg gcttgaacag ctcctcgtcc acgaagtcgg tataggaatc
     2281 gaagacggcg agggcctctc gcgcggatgc ctcgtcgaac gtatcccata tgacgatcgc
     2341 gccgtcgccc atcagcttat aataggagca accgtagccg aactggttga cgcaggacag
     2401 caggttcgag gtgaaatcct tgatcagggc gaagacctcg tcctcgtcgt gcgaacagag
     2461 gaagtcgctg aagttgcgca cgtcgacgca caacacgagg gcgtccggct tgacctcgcc
     2521 gaggtagacg tcgtcgaaca cgtcgaccgg catcttcctg agtcccgcca gggcggaggc
     2581 atcgagctcg acgcaggcgc cgccagcctg atcctccgga ggtgtattcg gttccatgac
     2641 gccattctac cagaaaggac ccgtccacag atacgccctt ccgtgataga cttcggcacc
     2701 atgtccaaca aacgcctagc cctgcgcgta acccgcaact ccgtagtgat gatcatagcg
     2761 gtctacctcg tcatgcaggt attcacctac ttccgcgacg gcctgatcct cggcgtctcg
     2821 ggcctgtccg acctcctcgc gatgacggcc gcgtacatgt tcagcgtcgt catccccccg
     2881 gccctggtac tcggcctgat cctgtacctg ctggccctgc gcatccagcg cgttgcctgt
     2941 gccctcgaac gcggcgagac cattccggac gatgaacttg agcgaacgag gaagcgcctg
     3001 atcggcttct ccaaggtcgt gctcgcagag aatatcgtag gcttcgcgtc gggttacgtg
     3061 atcttcgtcg tgtcgcaggg cttcctgcac gagatcctgc gcttcgacag gctggtcgtg
     3121 ctcgtttcaa acctagccgg cggcgtgatc tacgcctccg cccagaacgc cctgaacgag
     3181 atagtcttcg ccgatatacg cgaccgcctc ggcatccgcg agataggcga acgccggcga
     3241 gaacgctcga cgaccggcaa gcagatcggg ctatcggccg ccttagtcgt ctacgcggtc
     3301 accttcatcc agttcaacgt caaggatacg gtcgcattca acaaggtggg cgacgatata
     3361 ctagccggag tcgtctccgg ctccatcctg ccggaggatg ccgggaccgc ctaccgctcg
     3421 ctactcgccg agcggctacc cgagctcggc acgcgctcga gcgtcggggc cgacgacgta
     3481 ccgctaccct gggaacgcgc cgatagcgcc gtcaggcgcc aacagacgat gttcatcatg
     3541 tacgccgcct tcaccctatg catctgctgc gcggtgcagg ctatcgtatc gtcgagcctg
     3601 cgcagacagc tacaggccat cgccaaccga gtgaaggacg tgctcgacgg cggcggcgac
     3661 ctgcgcatac gcctgagcat acgagcgatg gacgacctcg gcgagctgac cgagctcctg
     3721 aaccgcctcc tggaccggtt ccactcgata gccagggaca taggccatac ggccaggcgg
     3781 accagggaaa gctccgaggc catagaccgc gagctgatcc gctcggagtc gctggcgcgg
     3841 ggcacccgcg agacggtacg ggaactcgag acgaccctac agtcccaggc ggccgcgacc
     3901 aggaccttcg tcgacgcgct acgctccttc agggacggcg tggccgcggc cgacgccgcc
     3961 accgaagacc agaagcgctt cgtagccgag acttcggccg ccatggagga gatggccgct
     4021 agcatccgct ccatctcctc gatgaccgag cgggccgggt cgctatccgg gcggctttcc
     4081 gagagcggcg acgccggtag cgccgcggtg cgcgacacca gggccgcgat agacgagata
     4141 aacgccgccg cccgcgaggt gctcggcgtg ctgacgacac tcaataagat atcggccgac
     4201 atcaacctac tcgctatgaa cgccgcgatc gaggcggccc acgcgggaga acacgggcag
     4261 ggcttcgccg tggtcgccga cgaggtccgt accctggccg ggaacgcggc ccagcgtact
     4321 cgcgccatca aggagctgat ccaagccatg ggagcgcgcg tcgacaacgg cgtacggcag
     4381 gcggaggcct caggggcggc cctggccggg ctcaccgccg gcctcggcga gtccgcctcg
     4441 atctcgcggg agatagccgc cgccgctaag gagcaggccg ccggcacgga cgccgtgata
     4501 ggctccatca cgcaggcggt cagatcggcc gatacggtca gcgcgatgat gggcgaccag
     4561 gccaggcgta gcgacgagat cgcgatggcc ctcgagggcg tgctacggcg actcgagggg
     4621 ctcgcggccg acgccagccg ccagacgcgc gaggtgaccg cgctcgagga gtcgttcgcg
     4681 gccgtcagga aggaagtcga cggcaacctc gaatcggtcg gcgcgctcgg ctcggagatg
     4741 gccaggttct cggtctagcc cgactgggcg gctaaccggc cgccagctcg ccaactatca
     4801 tcgcgcgcag ctcggacaag atcatcgcgg tggcgcccca gaccgaatag ccggctagct
     4861 cgtagcacgg ggcttcggcg acgccgaccg cgacgccgac cgcgacgccg tccgcgatgc
     4921 cgtccgcgat gccgtccccg acgccggccg cgacgccgag gcgtacgtcg acgcaggacg
     4981 ctccggccgg atcgagcagc tcgtcgagcc gcggctcgag cagcaccgcg acttcgctgc
     5041 cggacaggcg gaattcaggg cgcgcggccg cgacgccggc gaagggccga accaggaacc
     5101 tgctcggcgg gacatggagc ggagtcagcg cccgtaccag gcggaccgag ccggggtcca
     5161 ccccgatctc ctcgcgcgtc tcgcgtaggg cgcagtcctc ggggctttct tcaggctcgc
     5221 gcgccccgcc cggtagcgat acctgtccgg cgtggacgcc cggtccgtcc ggcctgacga
     5281 tcagcgggaa gcgtagtccg ccgccactct catagaacag taccagcacc gccgcctctc
     5341 gccacccggc tccggacgga acggcgggct ttggatcccc gaggcacgcg agcgcctcga
     5401 gctcctgccg gcggaacggc ggcgccatca cggttcgggc cgccgccccc ggcagcggcc
     5461 tgggcgggga gatggcttcg agcagttcgg tcacggtcat gcttcgagta tgaccccgag
     5521 cggaccgatg gggcaaggga gccgcgccca gggaagcgct cggccgcgcc ccgaacctcg
     5581 tctacccctg cgcgcgcggc ctcgccgacg actatgcgga acgattccgc ggcatgagcc
     5641 cggacgaggc cctagccctc ccgcgcctcc atgacgcgct ccacgagcgg ctcgacgacg
     5701 gaaacgagag cgtccttcgc cacgccttcg accttcagcg tgcagtaacc cgtcttgccg
     5761 tcatcgccgt cgaaggcgac gagggacagc aggttgccgc ccgcctcggc aatggcggaa
     5821 gcgatcgccg ccagttcgcc gcgcttctcg ggcatcagca gcgtcagccg cacaccacgg
     5881 tgccgcgcgc cgaagaggtc gatgaacagc ctgaataggt cggactccgt gatgatgccc
     5941 acgagctcgc cgccgcgcgt caccggcagg ccggcgatgc ggttgtcgtc catgatcctc
     6001 gcggcctcct cgaccggcag gtcctcggac acggtgaccg gatccttcgt catcaccgac
     6061 tccaccttga gcctggagat caggtagtgg agctcgtaga tgtcgagcga ggtggccggc
     6121 ggcgccgagg cgcgtcgcag gtccgaggcc gtgacgatgc cgagtagctc gcccttcttg
     6181 ccgaccaccg gcaggcggtg gatcttctcg cggcgcatca gggcttccgc ctccggtacg
     6241 gccgtatcgg cgcctacggt aaccaggtgc cggctcatgc gattgcctac ggtcatgttg
     6301 cctcctcgct gatcatagcc cgaggtacgc cttgcgtacc tcttcgttag ccaacagacg
     6361 ctcggccgag tcggccatct tgatcgagcc gttctgcagc acgtagccct tgtcggctat
     6421 cttgagcgcc atgttggcgt tctgctcgac gaggaacacg gtggtcctgc gttcctcgtt
     6481 gatgcgcttg ataatatcga aaatattctg gacgatcagc ggagccaggc ccagcgacgg
     6541 ctcgtcgagc agcaggatcc gcggcctggc catcagggcc cgagatatcg ccagcatctg
     6601 ctgctccccg ccggacagtg tcccgccctg ctggttccgc cgctccgcga gcctcgggaa
     6661 caggtcgaag acctcctcca ggtcgacgcg gaccgtggcc ttgtcgcgac gcaggaaggc
     6721 gcccatgtcc aggttctcca gcacggtcag ctgagggaag atgcgccggc cctccggcac
     6781 ctgggacagg cccatcgcga cgatggagtg agggctcttc ctcgagatgt cctcgccgtc
     6841 gagcagcacc tggccggagc gtatctgggc gatgccgcag atcgacatca gcgtggtggt
     6901 cttaccggcc ccgttggcgc cgatcagcgt gacgatctcg ccctcctcca ccgacagcga
     6961 gatgccgcgc agagcctgga tattgccata gtacgtgcag acgtccctga gttccatcaa
     7021 agccatgtca ttctcctaaa tacgccctga tgacctcggg attccccttg atctcctccg
     7081 gcgtgccctc ggctatcagc cgaccgtagt cgaggacgta gatgcgctcc gatacgttca
     7141 tcacgaggct catgtcgtgc tcgatgagca gtatcgtcac cttctcctcg tcgcgtatca
     7201 ggtcgatcgt ctcctcgagc tccttcgtct cgtgcgggtt catgcccgcg accggttcgt
     7261 cgagcagcaa gaggaagggg tcggtcgcca gggccctggc tatctcgagc cgccgctggg
     7321 cgccgtacgg caggttcctg gccgtctcgt tgatgaacgg gtcgagcttc atcttgacga
     7381 ggatgcggcg gcagtcgtct atcagccgcg cctcctcgtc gcgcgtcgcg gcgtccctgg
     7441 ctatggctcg gatgacgccg gcggcgagcg cgttgtgccg gcctatcatc acgttctcga
     7501 gcacggtcat attcccgaat agcctgatgt tctggaaggt gcgcgcgagg cccttgcggt
     7561 tgatgacgtt cggcttgagc ccctggatgg cttcgcgctg tccgccgccg cggcgtatgg
     7621 tcacgcggcc ctcggtcggt ttgtacacgc cggtgatgca gttgaagatg gtcgtcttgc
     7681 ccgcgccgtt cggaccgatc aaggcgcaga tctcgccctc gcgaatatgg agcgaaacgt
     7741 cgtcgatggc ccggaggccg ccgaagacca tcgacagccc gtcgatctcg aggatgggat
     7801 tggccacgct catcggcggg cctcctggtc gccggccgcg accggcgcgc cgactgctga
     7861 tggcgagcca tcgaaggtgt acttcttgcg gttcctgggt aacaggccgt cgggcttgaa
     7921 gatgatcaca agtatcatcg cgatcccgta gatcagcatg cggtactggg cgagcggcct
     7981 gaagtactcc ggtagtagct tgagcaggag ggcgccgccg atggcgcccg gtatcgaacc
     8041 ggtgccgccg atcacgacgg ccatcagcac cgtgatcgat tcccagagcg taaagctatc
     8101 cgggttgatg tacgtggtct gcgcggccag gaggacgccg gcgatgcccg cccagaacgc
     8161 cccgagcgag aaggtgatca gtttgttgcg caccaagtcg acgcccatcg cctcgcaggc
     8221 gatctcgtcc tcgcgcatcg cctcgagggc gcggccgacc cgcgaatcct cgatgcgcct
     8281 gacgacgaag acggtcaggg cgaccaacgc gacggcgatg tagtagatat aggtggcggc
     8341 ctgcctgggc ggcaggcgca ggccgaagaa ccacggccgc gggatcaggc tgatgccggt
     8401 cgcgccgccg gtcacctcgc cggaattctg cagcaccatg cgcacgatct cgccgaaggc
     8461 gagcgtaatg atggccaggt agtcgccgcg cagcctgagt accggcaggg acaggagtgc
     8521 accgaaaatg gcggccagga cgcccgcggt cggcagcgct atccagaaca accagccggg
     8581 atcgaggccg gcggcgacga aggccgggtc ggcgtacttc cacaggagcc cgtaggtata
     8641 ggccccgacc gcgaagaacg cggcgtagcc gaggttcagg agccctccga ggccgaccac
     8701 gatgttgagg ccgagcgcca ggatcacgta gatcagcgcc gtgatcatga tgttcgtatg
     8761 gtacatgccg agcgcg
//
LOCUS       DAOPHV010000034         8771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_10039_length_8771_cov_4.121271, whole genome shotgun sequence.
ACCESSION   DAOPHV010000034 DAOPHV010000000
VERSION     DAOPHV010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8771
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10039_length_8771_cov_4.121271"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..7931
                     /locus_tag="PLQ29_01725"
     CDS             324..7931
                     /locus_tag="PLQ29_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85391.1"
                     /translation="MTKGGVDTAGAMADLSSAVEKAAGLAPTWMADELRRWDEAMLDY
                     VVARSVAVGAAPDGSAEAYANKAAERLASARRLGASTRAMSEAGDHGLASLYELAHDS
                     SFSASEASAIRHEALTTIATAIAGMDGDGAALVAARFGYLEQNDMAIVVKDAARIRLE
                     DAWSSWDYAATAEAARLATGPLARIAYTMESMVFAMLERDYGAVSREGLVSLLSPGAD
                     GAASWLGSYYASEGQDAGTTMESLIVAAAGEAGDAWSYEAARSRVTAMVVDSLDTLAS
                     HIREARGHLGDASAPIERYLAAIVASTAPDFEFVSWILPLVGLAGSYGQSLSLGASED
                     AAMASAFIGLGDTAMAFSAASFYLAGLAEAGRTSEFDSLASLVSAEKAERGRAVIDRA
                     EACSAYLGDFSGGIIAWALKASGGDVGMALAMVASIEGASSSNSLEGPFGRWLFALTS
                     LKIDASFAEAERLKDLSAYSAAYDRHIAEAEAAEAGGILHWRELLGSNALRAAIEADA
                     SRLPEPRAPGEPEAEPTETPAVAASWNAGALADGSDAAHRRTLALNDGLASWKRYSES
                     IGMVGGDQLASLVVSMERYTSDPSTPYDASGLPRASSSVARASQAALDAYLAFDSSRA
                     LAAARFGELGSMAGAVGDADAMIELARGLEIEIEAARGGQRVALAAYESAGAKLAEAG
                     AAYDEAYSQASRRYDDLEEANHAYAIEDAIWRWASSAYLYASDEACSGSLYGDPAGEL
                     SYCEASLARASTAMAALRGLYDEGQEVREYASSEYRAAYDEYRESYRRLMLVRKAEAA
                     LADGLTLELAANEKAAAAYRAARSGLATGRTVDDGFAAYLRIGEDGCIRLAYDASFAF
                     SGAMGQSELSAFFSAQAQGDTGSRMSETAFETNLDRLASWMTSVSFDRTRAERWGLAR
                     DYVVKRLAANDSGFASRVSYSESAIDDATIGGHRFTLAGPTLAEILATYREDSLAADQ
                     EAAYASLDGDEKAMFDTYLALQLIGAMKPPKATNDGSSTYDAFSSWSDRAQYAALEAE
                     AERQIAVCRTGIEASAATYAGFLAAAAVAGAFLITIFLVPGLLAAAALALATMTAFGL
                     TMSGVGETRAVYSSAMSDLFAQTESGALRMTDGMPSCVALRSKYLSSCDRLASLEGVT
                     LGGAACDESSLRAALAQSGSLTSAEVDELTSLYGAYASETGATNHGSLEALSALATWS
                     RVSRDDSLGGLEAAYAEDDVERVEAGEIYNAVYAAFIAGRASEEDLALAANAAFGPGA
                     ASAKTHVSNLETVLREGSLSGESAAGSEGFDAARQYSTLSARAVSLRYDAELSAREAE
                     WTCDRQELYERWLAWRRAGELLLSRGESDFDAAFERLESEAGSWAERFAEAYSIKAAA
                     WDYAYADSLEAKRDWAARATMAVDGAASEAIVAMLGVDAAAAGRKLTSFAVTGFDFED
                     SSYGAYERVIGGSGLARLGESLEAWSLSAGGLSAKVRAGLAGGRVWESGSILAAAADF
                     ALSANESLAGIQAGIMASELGRSIEKAKVSLVDRIAYANRGFDDDMNGLFVADGAWRR
                     DGTRYGKDVVVHSTVGQAFITERVEVPVFAYFELSPWSLGDTLSEASLRGSGASIARA
                     RMASAQAAVEEKGREVFGAGDGRGTFGEHVGTSPERLADANPDDGIECFFSDAGSGEL
                     GRLMSALVYWSVVEGRGWAEANKPIYDKDLWDDRDDWLEAPTLREVADVGVSVVTGLA
                     TGGIGALAMNLADDMLFGALDGLCGYTSWAEASLSFGKKAVGSLANYGTAGLFDGAIA
                     SVAGLGTLRSGFDAAVLSGARGFTELASSGLVSSVKLDYEADGSLAGLSIDKDRFVES
                     VFGEGAIAGLVGSMAGAACGSSMDYGGAFEDAVWSGFTDLASIAATESASYATHLAFS
                     SYRGVSGAALFEDAWSHSAGISLNVANARSVLGVAGFLGAVFGDGYDAAARMESERVA
                     SSFGSIGLSISIGSGGVAGSLGGGGYDLIDIGTSIGKGLAFDASLGRFAADRGSTLRG
                     ALETAYAFGDLAAEETVWRLIAGQDELRFDSLAGKAVTVADGLGNRTITVSPAGDDRG
                     QTAMLELAIGLQHEAWRNGRDDGAIGQAYETLSAVTAHTELAMRLAGSSEFGGAMIGL
                     MGLDSIFGADIAHYSGGSFTSYVAEAYDSIGDNWKLTMDGRLLNDGRARVLAEILNDD
                     GSPGWVEVDGSAFESSTAAALVAYLGEARALELFGGSVYDASRYDDQTLQDVLGLDPV
                     DVKAIRRSPSEAASVIALASDESRRRLLGEALMKGAGIVWDASAQGGNGAWIGEGLGL
                     SLTDGEIHGSAALRSIGSGAYERYSITSEVERGYGAYDVWMDGVKHSLEGGLNRLSYT
                     KWDIDSGEQLATLVAGGVWNTVDNSYGQLNAAGVPIGTDVRYSLLDGPTIQAYTIARG
                     SFVSRWSLLGSRKFEGQDVLILSDATTVAGERLDSAGKRVGYPYDDRWLIHSTDWGSS
                     DGCLVYSKTQANVNYYEVLMAQLKSWGLYQGYAIAGILSDESDFVYGGGYKEGTW"
     gene            7931..>8771
                     /locus_tag="PLQ29_01730"
     CDS             7931..>8771
                     /locus_tag="PLQ29_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85392.1"
                     /translation="MSIMTVLLMALCASCLGAQGFVDADYLIPVDHRMGLPGLEPFNY
                     VYPADYFPLHGVLYGGYLIIENHIKPHLYSILTYDDGVTADNGYRYYTTGIVDWHDPA
                     IITSGGDMLLVVERYIYEDPDEHIAGIDREGSGAFFGGSKRFKDAIIQDWDSYYQGWG
                     EFRPGVWEDGIESIVAWSTLTETVKGEIVEYGVDRLRYPFYSFIDAELCFNNAMLFWA
                     EGEPGSGIGGTIHVAFNRSSDHVMILNGAVDFARLNLYKDNNRAKRVLVRADDGSFAF
                     DGFA"
BASE COUNT         1527 a         2564 c         3002 g         1678 t
ORIGIN      
        1 ctgcgacgat cgagtcgttc tcgtccgctc tccagtcgct cgcggccgac gatccgtacg
       61 tcggggaatt cgccagggga cggggcttct tcgccgtggc cggtagcgat ctgtcgtcga
      121 tactgaccat agatgagtcg gcggccctcg agcgtgccag aggcatgctg acggcatacg
      181 aacggtatgg gcgggaatcg ctcgcgttgg agcgggacag gttcgagaag gcgacctcgg
      241 ccttgacaga agcgctcttt ccgctagggc tgaccctagg cgacggacgc tccttgccgg
      301 cgtctcgcga tatcgccgcc gcggtgacga agggcggcgt cgatacggcc ggcgccatgg
      361 cggacctttc ctcggccgtc gagaaggccg ccgggctggc gccgacatgg atggcggacg
      421 aactgcgtcg ttgggacgag gcgatgctcg actacgtggt ggccaggagc gtcgccgtgg
      481 gtgcggcccc ggatggtagc gccgaagcgt acgcgaacaa ggccgccgag aggcttgcgt
      541 cggcccgacg cctcggggcc tcgactcgag cgatgtccga agcaggggac catggactgg
      601 cctcgctcta cgagctcgcc cacgattctt cgtttagcgc gtccgaggca tcggcgatcc
      661 gccacgaggc cctaaccacg atcgcgacag ccatagcggg aatggacggc gatggtgccg
      721 ctctggtggc agcccgtttc ggctacttag agcagaacga catggccata gtcgtcaagg
      781 atgctgcgcg tatacggctc gaggacgcct ggtcgtcgtg ggactacgcg gcgacggcgg
      841 aagccgcccg cctcgccact ggcccgctgg ctcgaatcgc ctatacgatg gagtcgatgg
      901 ttttcgccat gctcgaacga gattatgggg ccgtatcgcg tgagggcctc gtgtcgctcc
      961 tgtcgcctgg agcggacgga gcggcgagct ggctaggatc gtactacgcc agcgaaggcc
     1021 aagacgccgg gacgacgatg gaatcgctca tcgtcgcggc ggccggcgag gccggagacg
     1081 cttggagcta cgaggccgcg cgatctcggg tgacagccat ggtcgtcgat tcgctcgata
     1141 cactcgcatc ccatatacgc gaggctcgtg gacacctggg agacgcgtcg gctccgatag
     1201 aacgctacct ggccgctatc gtcgcctcga ccgctccaga cttcgagttc gtctcgtgga
     1261 tcttgcccct cgtcggactt gctggttcgt atggccaatc tttgtctctc ggagcttcgg
     1321 aagacgcggc gatggcctcg gccttcatcg ggctcggcga tacggcgatg gccttcagcg
     1381 cggcctcgtt ttacctagcc ggtctagccg aggcggggcg aacctccgag ttcgattcgc
     1441 tggcgtcgct cgtaagcgcg gagaaggccg agcgcgggcg ggccgtgatc gaccgcgccg
     1501 aggcttgctc cgcctacttg ggcgatttct ccgggggcat catcgcctgg gccttgaaag
     1561 ccagcggagg tgacgtcggc atggccctcg cgatggtagc gtccatagaa ggggcgtctt
     1621 cttccaactc gttggagggg ccgttcggtc gttggctatt cgcgctgacg agcctgaaga
     1681 tagacgctag cttcgccgag gccgagcgcc tgaaggactt gtcggcctat tcggccgcct
     1741 acgatcgtca tatagcggaa gccgaggcgg ccgaggctgg cggaattctc cattggcgag
     1801 agttgcttgg gtctaacgcc ttacgggcgg ccattgaagc cgatgcgtcg cgcctgcctg
     1861 agcctcgagc tccgggcgag cccgaggccg agcctaccga aacgccggct gtcgcggcct
     1921 catggaacgc cggcgcgctc gccgacgggt cggacgcggc acataggcgg accctggcct
     1981 tgaacgatgg cctcgcctcg tggaagcgat actcggagag tatcggcatg gtgggtggtg
     2041 accagcttgc tagccttgta gtgtccatgg agcggtatac cagcgatcca tccacgccct
     2101 acgacgcctc gggtttgccc cgcgcctcga gctcggtggc ccgagctagt caggcagcct
     2161 tggacgccta tctggccttc gattcgtctc gagctctcgc cgccgcaagg ttcggagaac
     2221 tgggatcgat ggccggggcg gtcggtgatg ccgatgccat gatcgagttg gcgcgcggcc
     2281 tcgagatcga gatcgaggcc gcgcgcggag ggcaacgagt cgccttggcc gcgtacgaaa
     2341 gtgccggcgc caagctcgcc gaggccggag ccgcatatga cgaggcgtat agccaagcct
     2401 cgcggcgata cgacgacctc gaggaggcga accacgcgta tgccatcgag gacgcaatct
     2461 ggcgttgggc tagttcggct tacctctacg cttcagacga agcgtgctca ggctcgctgt
     2521 acggcgatcc agccggggag ctgtcatact gcgaggcgtc gctggctcgt gcctctacgg
     2581 ccatggccgc gctacgcggg ctctatgacg agggccagga ggtgcgcgaa tatgcgtcga
     2641 gcgaataccg tgccgcgtac gacgagtatc gagagagcta tcggcgccta atgctcgtca
     2701 gaaaagccga agcggccctg gccgacggcc tgaccctcga gttggccgcc aatgaaaaag
     2761 ccgccgcggc ctacagagcc gcgaggtcgg gcctggccac tgggcgaacg gtcgacgacg
     2821 gcttcgccgc atacctgcgt attggagagg acggctgtat ccgcctggcc tacgacgcgt
     2881 ccttcgcgtt ctccggcgcg atgggccaaa gcgagctgtc ggcgttcttc tcggctcagg
     2941 cgcagggcga taccggttcg aggatgtccg agaccgcgtt cgagacgaat ctcgataggc
     3001 ttgcctcctg gatgacgagc gtctcgttcg atcgtaccag ggccgagcgc tgggggctcg
     3061 cccgcgatta cgtcgttaag cgcttagccg cgaacgactc aggcttcgcg tcacgagttt
     3121 cgtattccga atcggccatc gacgacgcca cgatcggggg acatcgcttc acgctggcgg
     3181 gtcctacgct tgcggagata ctcgcaacgt atcgggagga ttcgctcgcg gccgaccagg
     3241 aggccgccta cgccagcctc gatggcgacg agaaggccat gttcgatacc tatctggcgt
     3301 tgcagctgat cggggcgatg aagccgccca aggctaccaa cgacggttcg tcgacctacg
     3361 acgccttctc gagttggtct gaccgggccc agtatgccgc gctcgaggcc gaggccgaac
     3421 gtcagatcgc cgtatgccgt accggcatcg aagcgagcgc ggctacgtac gccgggtttc
     3481 tcgccgccgc cgccgttgcc ggagccttct tgatcaccat tttcctcgtt cccgggttac
     3541 tagccgccgc ggctcttgcc ttggccacga tgacggcgtt cgggttaacc atgtccggcg
     3601 tcggcgagac ccgagcggtc tactcatcgg ccatgtcgga cctattcgcc cagaccgagt
     3661 ccggtgccct tcgcatgacg gacggcatgc cttcgtgcgt agcgttacga tcgaagtacc
     3721 tatcctcgtg cgatcggctg gcctcgctcg agggcgtcac ccttggggga gccgcgtgcg
     3781 atgagtcgag cctgagagcc gcgttggcgc agtccggctc gctgacgtct gccgaggtcg
     3841 acgagttgac ctcgttgtat ggcgcctatg cctctgaaac cggggcgacc aaccatggtt
     3901 cgctcgaagc tctgtcagcg ctcgcgacat ggagccgggt gtcacgcgac gactcgctcg
     3961 gcggactgga ggcggcgtat gccgaagacg atgtggaacg cgtcgaggcg ggagagatat
     4021 ataacgccgt ctatgccgcc ttcatcgccg gacgcgcctc ggaagaggac cttgctcttg
     4081 cggccaacgc cgccttcggg ccgggcgcgg cgtccgccaa aacgcatgta tccaatctcg
     4141 agacggtctt gcgtgaagga tccctgagcg gcgaatcggc cgccggttcc gaaggctttg
     4201 acgccgctcg tcagtattcg actttgagcg ctcgagccgt atcgcttcgc tacgacgcgg
     4261 aactgtccgc cagggaggct gagtggacct gcgatcgtca ggagttgtac gagcgatggc
     4321 tagcgtggcg acgggcaggc gaactactgt tgtcgcgcgg ggagtctgac ttcgatgcgg
     4381 cgtttgagag gcttgaatcc gaggccgggt cctgggccga gcgcttcgcc gaggcgtact
     4441 cgatcaaggc ggccgcgtgg gactatgcct acgccgattc gctcgaggcc aagcgggatt
     4501 gggcggccag ggcgacgatg gccgtggatg gagccgcgtc cgaggccatc gttgcgatgc
     4561 tcggagtcga tgcagcggcg gccggcagga agctcacctc gttcgccgtg actggattcg
     4621 attttgagga tagtagctat ggagcttacg agcgtgtcat cggcggtagc ggcctcgcga
     4681 ggctgggtga gtcgctcgag gcctggtccc tatcggctgg cggcttaagc gccaaggtta
     4741 gagcgggact cgccggcggt cgcgtttggg aatcgggaag tatcctcgcg gcggcggccg
     4801 atttcgcgtt gtccgcgaac gagagcttgg ctggtataca ggccgggata atggcttcgg
     4861 aactcgggcg ctctatcgag aaagcgaaag tatcgctcgt cgacagaatt gcatacgcca
     4921 accgaggctt cgacgacgat atgaacggcc tgtttgtcgc cgacggcgcc tggaggcgcg
     4981 acggcacacg ctacggcaaa gacgttgtcg tccactctac cgtcggacaa gcatttataa
     5041 cggaacgcgt cgaggtaccc gtgttcgcgt acttcgagct ctcgccctgg agcctagggg
     5101 atactctttc ggaggcatcg ctacgaggga gcggcgcgtc gatagcgcga gcgcgcatgg
     5161 cgagcgcgca ggccgcggtc gaagagaagg ggcgtgaggt cttcggggcg ggagacgggc
     5221 gcgggacgtt cggagagcac gtcggaacat cgcccgaacg cttggccgat gccaaccccg
     5281 atgacggaat tgagtgtttc ttctcggatg cgggctcggg cgagctcggt cgcttgatgt
     5341 cggctctcgt ctactggtcg gtcgtcgagg gccggggctg ggccgaggct aacaagccga
     5401 tctacgataa ggacctgtgg gacgataggg acgactggct cgaagcgccg acgctacgcg
     5461 aggtcgccga cgtcggcgta tcggtcgtca cgggcttggc gaccggcggc ataggcgcct
     5521 tggcgatgaa cctggccgat gacatgctgt tcggcgcctt ggatgggctg tgcgggtata
     5581 cgtcatgggc tgaggccagc cttagcttcg ggaagaaagc cgtcgggtcg ctggccaatt
     5641 acggcacagc cgggctcttc gacggggcca tcgcgagcgt agccggcctg ggtacgctac
     5701 gctcgggatt cgacgccgcc gtgttgagcg gcgcgcgcgg cttcaccgag ctcgcgtcgt
     5761 ccggcctcgt ctcgtcggtc aagctcgact acgaggccga tggatccctg gcggggctga
     5821 gcatcgataa ggatcgcttc gtggagtctg ttttcggaga aggcgcgatc gccggattgg
     5881 taggtagtat ggcaggagcc gcctgcggat cgtcgatgga ctacggcggc gctttcgagg
     5941 atgcagtatg gtcaggcttt accgacctgg cctcgatcgc ggcgaccgag agcgcctcat
     6001 acgccacgca cctggccttt tcgtcgtacc gcggcgtatc tggggcggcg ctgttcgagg
     6061 acgcgtggtc gcatagcgcg ggaatcagcc tgaacgtcgc taacgctcgt agcgtactcg
     6121 gggtggccgg cttcctcggt gctgtcttcg gagacggcta cgatgcggcc gcccggatgg
     6181 agagcgagcg ggtcgcaagc tccttcggct cgataggcct atccatcagt atcggatcgg
     6241 gcggggttgc gggatcgctc ggaggaggcg gctacgacct gatcgacatc ggaacgtcga
     6301 taggcaaggg cttggccttc gacgctagcc taggtcgctt cgccgcggat cgaggctcga
     6361 cgttgcgcgg cgcgctcgag acggcgtacg ccttcggaga cctggctgcc gaggaaacgg
     6421 tatggcgcct catagcgggc caagacgagc tgcggttcga cagcctggcg ggaaaggcag
     6481 taacggtcgc cgatggcttg gggaatcgta ccatcaccgt cagcccagcg ggcgacgatc
     6541 gcggtcagac ggccatgctt gagctggcca ttggtctaca gcacgaggct tggcgaaacg
     6601 gtcgcgacga cggagcgata gggcaggcct acgagacgct gagtgccgtg acggcccata
     6661 cggaactagc catgaggctc gcgggcagtt ccgaattcgg tggcgcgatg atcggtctca
     6721 tgggcttgga ctcgatcttc ggggcagata ttgctcacta ctcgggcgga agctttactt
     6781 cgtacgtcgc agaggcctat gattctatcg gggataattg gaagctgaca atggacgggc
     6841 gactcctcaa cgacggcagg gcgcgtgtgc tcgcggagat cctgaacgat gacggttccc
     6901 caggctgggt cgaggtcgac ggctctgcct tcgagtcatc gaccgcggcg gccttggtcg
     6961 cgtacttggg agaggcccgc gccctcgagt tgttcggcgg atccgtctac gatgcgtcca
     7021 ggtacgacga ccagaccttg caggacgtac tcggcctcga cccggtcgac gtcaaggcta
     7081 tacgtcgaag ccctagtgag gccgcgagcg tcatagcgct cgccagcgac gagtccagac
     7141 gacgtctgct cggcgaggcg ctcatgaaag gcgctggcat cgtctgggat gcgtcggccc
     7201 agggcggtaa cggcgcctgg ataggcgagg gcctcgggct cagcctgacc gacggtgaga
     7261 tccatggctc agccgccctg cggtcgatag gaagcggcgc gtacgagcgc tattccatca
     7321 cctcggaggt cgaacgcggt tacggggcct atgacgtgtg gatggacggt gtcaagcata
     7381 gcctggaagg gggcttgaat cgcttgagct ataccaagtg ggacatcgac tcgggagaac
     7441 agctagcgac gctcgttgcc ggcggcgtat ggaacaccgt cgacaactcg tatggacagt
     7501 tgaacgctgc cggggtcccg atcggtaccg atgtgcgcta ctcgttgctc gatgggccga
     7561 cgatacaggc atataccata gcgcgcggct cgttcgtctc gcgctggtca ctattaggat
     7621 caaggaaatt cgaaggccag gacgtgctga tcctgtccga cgcgacgacc gtggccggcg
     7681 agcggctcga ctcggcagga aagcgcgtcg gttatccgta tgacgatcgc tggctgatac
     7741 atagtaccga ttggggctct agcgacggct gtttggtata ttcgaagact caagcaaacg
     7801 tcaattacta tgaggttctt atggctcagc tcaagagctg gggactgtac cagggttacg
     7861 ccatagccgg gattctgtcc gatgagtctg acttcgtcta tggcggcggc tataaggaag
     7921 ggacgtggtg atgagcatca tgacggtttt actcatggcg ctctgcgcct cgtgtctcgg
     7981 ggcccagggc ttcgtcgacg ccgactacct gatcccggtg gatcaccgca tgggcctacc
     8041 agggctcgag ccgttcaact atgtgtatcc tgcggattat tttccacttc atggtgtgct
     8101 ttatggcggg taccttatta ttgagaacca tataaagcct cacttatatt caatcttgac
     8161 ctacgatgac ggcgttacag ctgataatgg atataggtat tacaccacag ggattgtcga
     8221 ttggcatgat cctgcaatca ttactagcgg aggcgatatg cttctcgtcg tcgaacgcta
     8281 tatctacgag gatccagatg agcatattgc agggatagat agagaaggtt ctggtgcctt
     8341 tttcgggggc tcgaagcgct ttaaggatgc aattatccaa gactgggatt cgtactatca
     8401 aggctggggc gagttccgac ccggggtctg ggaagacggc atcgaatcga tcgtggcttg
     8461 gtccacgctg accgaaacgg tcaagggtga aatcgtcgag tacggagtcg accggctacg
     8521 ctatccgttc tacagcttca tcgacgccga gctgtgcttc aacaacgcca tgctgttctg
     8581 ggccgaggga gagcccggtt cgggtatcgg ggggacgata cacgtagcgt tcaaccggtc
     8641 gagcgaccac gttatgatcc tgaatggcgc cgtcgacttc gcgcggctca atctctacaa
     8701 ggataacaat agggctaagc gcgtgctcgt gcgtgccgac gacggctcgt tcgcgttcga
     8761 cggcttcgcc g
//
LOCUS       DAOPHV010000035         8740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_10088_length_8740_cov_3.765688, whole genome shotgun sequence.
ACCESSION   DAOPHV010000035 DAOPHV010000000
VERSION     DAOPHV010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10088_length_8740_cov_3.765688"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="PLQ29_01735"
     CDS             complement(<1..1093)
                     /locus_tag="PLQ29_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPG85393.1"
                     /translation="MKVTFKLNGRTVAWDCEPGETLRSCLRRKGVVSVRDGCDGEGSC
                     GLCAVILDGRLVNSCQILAPQAEGKAVLTVDHFSKDRKLSIVQRALIDAHCVQCGYCT
                     PAVVLALHDLLERSPRPTKDETRDALSGTLCRCTGYEQFFDAARIASERLADPNYEVP
                     AGQEFRPDLRHVGKDREKVDAAALARGEKAFVEDRVDADACHLKLLMSPHAHAWIKRI
                     DVTKALEMPGVIDIVTHENCPDRVYTTAGQGYPEPSPYDQRMFPRKVRHVGDRVAAVV
                     AETPEQAEAALKAIVVEYEVLKPVLSIADAKAEGAPVVHHGDVSYVVGEAPKGSSTKG
                     DDRDDPIIYQFPLHADPHRNLAASVADGIG"
     gene            complement(1090..1890)
                     /locus_tag="PLQ29_01740"
     CDS             complement(1090..1890)
                     /locus_tag="PLQ29_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPG85394.1"
                     /translation="MVKEYYRPESLGEALDLLARPGALALAGGTYALAFEARDKPERV
                     VDLASVLPRAVEAKGGALSIGAGATFQELIESEAIPSAIKAAAATMSNRNTRNRATVG
                     GNLGANKSCSSLAPVLLALGASATIAERGKAPATTSLESWIAAPRGVILSVEVRLEPG
                     LKVAALRHSRTACDVATATAACAFRLDGGKVAGLRIALGGFGPRATLRPDLAALFEGR
                     PLPPKTDIEKSVSPLLSAISDVRGSAEYKRLRGAALVADALHAAEDLS"
     gene            complement(1884..3287)
                     /locus_tag="PLQ29_01745"
     CDS             complement(1884..3287)
                     /locus_tag="PLQ29_01745"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HPG85395.1"
                     /translation="MILLKDCFRVVRPRYGAGADAGSVGDDLSGVDVLIDGKRIAKIG
                     KALSVPAGDCKVIDASRHVVMPGLVNTHHHYYQTLTRNVPAVQDAKLFDWLVYLYEVW
                     KGIDEEAVYWSSMLAMAELAKTGCTLSTDHHYLYPRSFTGDLPGIQFKAAADIGLRFA
                     PTRGSMSRSKKDGGLPPDSVVQDEDEILAQSEEALRKYHDAAPDSMRKVALAPCSPFS
                     VSEQSMRDAADLARRYGARLHTHLAETNDEDDFCVQMYGRRPLKVMEDCGFVGKDVWY
                     AHGIHFNDDELDYLARTGTGVAHCPSSNMRLGSGICRVREMLDRGVAVGLAVDGSASN
                     DASDMLGEARQALLLQRVRYGSGGITAREVLGVATIGGARILGYEDAGSMEIGALADI
                     ALFDVMRLEYAGALSDPAAALLFSGYNHGVDHLVVNGEMVVENGRLVGVDEDRIRNEA
                     NRCAKGLYEKAGIQAAW"
     gene            complement(3284..4741)
                     /locus_tag="PLQ29_01750"
     CDS             complement(3284..4741)
                     /locus_tag="PLQ29_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPG85396.1"
                     /translation="MIDLRIDEQARKKNIQRCREKGIILPTYEQMRDPTKIPQSVKDE
                     LKGIGLWDVHSRNLFRVNWHNEPKEQGGQFGEVNYLEIPREITGTKARIVGLVGKWFP
                     TGAHKVGAALSCLLPELVTGRFDPTTKKAVWPSTGNYCRGGAYISRLLSCPSVAILPA
                     GMSKERFDWLRTMAEEVIATPGCESNVKEIFDTCADIEKNRPECVIFNQFDQLPNHLW
                     HYSVTGPAMAEVFKKIAKPGDHVSGAVLSSGSAGTMGSGSYLRDTFPGAKVGVAEALQ
                     CPTILENGFGDHRIEGIGDKHIPWIHNLRDTDAAIGVDDELPIRFIRLFNEPAGHKVL
                     AEMGVPADTIARLNLLGISGVGNLVAAIKMAKYYEMDENDVVFTMFTDSMAMYGSRIS
                     EMNAERGAYGQRQADKDYDRIQGLSVDYVLELSQVDKRRVHQLKYFSWVEQLGKNVDE
                     LRAQWSDYRSYWGGLRAQAADLDAMINDFNAEVVR"
     gene            complement(4899..6101)
                     /locus_tag="PLQ29_01755"
     CDS             complement(4899..6101)
                     /locus_tag="PLQ29_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPG85397.1"
                     /translation="MRWFYKCPGCGAEYEIEPGRYLCDACAARQRPDRPLEGVLECAW
                     EGSFAGSPDDIPLPVAKEHLPPLPVGDTPLWEPHRLRAETGYPRLFLKDDTCEPTGSF
                     KDRASYLVAGFAKERGITEIALASTGNAASSMSGIGAAAGLKITVFLPKKAPVAKRVQ
                     VLQYGAELREVDGTYDVAFDQSLAYSRETGVLSRNTAYNPLTIEGKKTVSFELVRDLA
                     RAREHEAKRSGHLAELAKKDYVAPDHVFVPVGDGVIIAGVYKGFEDLVRLGRIQKVPT
                     IWACQAEGSSAIARALAAGDFGEPVPSDTVADSISVDVPRNGAHALAKLKAHGGKAVV
                     VTDAEILAAQKELAAKAGLFAEPSSSAAWAGFVKAKAGVRPDETVVVMLTGSGLKDVA
                     GAMKGLGL"
     gene            complement(6286..7506)
                     /locus_tag="PLQ29_01760"
     CDS             complement(6286..7506)
                     /locus_tag="PLQ29_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HPG85398.1"
                     /translation="MSVQNGKEIRAKAEAYKDWTAQNLSSIIKVPAMSNKEKERIMFL
                     KGMCEKAGFDEVRIDGLGNLLARVGSGKKILAIDAHIDTVGIGDRSQWKKDPFSGDIE
                     NGLVHGRGTSDQTGGAAAMITAGRILKELKYAGDYSVWFVFSIIEEDCDGMCWKYLIE
                     EEKFVPDFFVSTEPTSLRLYRGHRGRMEMEVHLKGVSCHGSAPERGVSAAYKAARAAL
                     AIEALNAALKPDDDNFLGKGTVTVSQMKVQGPSQCAVPDEAMLYLDRRLTWGETKDLA
                     IQQVKDAVLAAGVDAVEVTMPEYAIPSWTGKDYRQELYFPTWKFPADHPFVKAGVAAH
                     EAVFTAAPVVDKWTFSTNAVAVAGRHGIPSIGFGPGDENQAHAPNEITRVDDLWKAAA
                     FYAALPYALEEAKL"
     gene            complement(7503..>8740)
                     /gene="ssnA"
                     /locus_tag="PLQ29_01765"
     CDS             complement(7503..>8740)
                     /gene="ssnA"
                     /locus_tag="PLQ29_01765"
                     /inference="COORDINATES: protein motif:HMM:NF005540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HPG85399.1"
                     /translation="IVEVGRGLAARHPGAERLSPGGYVSPGLVCSHNHFYSALSRGLM
                     VAIQPSKDFAQQLKNMWWLLDRAIDEGITRASGLAGAADAAMLGVTSVVDHHASPEFI
                     DGSLDALAEGFETVGLRGVLCYETTDRNGREGAQAGIRENERFARRVDDERKAGGDPL
                     IEAAIGAHAPFTVDDETLSGLAAACSSTGRGLHIHVAEDKFDAVDSRYRFGKDICVRL
                     DDAGLLGPKTILGHGLYLTPSEVELVNARDSFIAHNARSNMNNCVGYNDKIGQYRNPV
                     LGTDGIGSDMLEEACFAFFKHHDAGGTRWMDAYLEMLQNGNRLLERYFPGKRFGRVEA
                     GYEADLTFWDYDPPTPLESGNVAGHVAFGMASRMVSGTMVAGRFIVKDRKPVFDAAAI
                     AQRGRDETRRLWQRMEERK"
BASE COUNT         1304 a         2817 c         3007 g         1612 t
ORIGIN      
        1 cgccgatgcc gtcggccacg ctggccgcga ggttgcggtg cgggtcggcg tggagcggga
       61 actggtagat gatcgggtcg tcgcggtcgt cgcccttcgt ggagctgccc ttcggggcct
      121 cgccgacgac gtacgatacg tcgccgtggt gcacgaccgg cgctccctcg gccttggcgt
      181 ccgcgatgga caggacgggc ttgagcacct cgtactcgac gacgatggct ttgagcgcgg
      241 cctcggcctg ctcgggagtc tcggccacga cggccgcgac gcggtcgccg acgtgacgga
      301 ccttgcgggg gaacatgcgc tggtcgtagg ggctcggctc cgggtagccc tggccggcgg
      361 tcgtgtagac gcggtcgggg cagttctcat gggtgacgat gtcgatgacg ccgggcatct
      421 cgagggcttt ggtaacgtcg atgcgcttga tccacgcgtg cgcgtgcggc gacatgagca
      481 gcttgaggtg acaggcgtcc gcgtcgacgc ggtcctcgac gaaggccttc tcgccgcgcg
      541 ccagtgccgc agcgtctacc ttctcgcggt ccttgccgac gtgccggagg tcggggcgga
      601 actcctgccc ggccgggacc tcgtagttcg ggtcggccag gcgctcggag gcgatgcgcg
      661 cggcgtcgaa gaattgctcg taacccgtgc agcggcacag cgtgcccgac agcgcgtcgc
      721 gcgtctcatc cttggtcggc ctcgggctgc gctccagaag gtcgtggagc gccaggacga
      781 cggccggggt gcagtagccg cactggacgc agtgcgcgtc gatgagggcg cgctgcacga
      841 tcgatagctt gcggtccttc gagaagtggt cgacggtcag gaccgccttg ccctcggcct
      901 ggggcgccag gatctggcag gagttgacga ggcggccatc gaggatgacg gcgcagaggc
      961 cgcaggagcc ctcgccgtcg cagccatcgc gcaccgatac gacgcccttg cgcctcaggc
     1021 aggagcgtag cgtctctccg ggctcgcagt cccaggcgac ggttcggccg ttcagcttga
     1081 atgtaacctt catgacagat cctccgcggc gtgtagcgcg tcggcgacga gcgccgcccc
     1141 acgcagccgc ttgtactcgg cgctacccct gacgtcggag atcgccgaca ggagcgggga
     1201 aacggacttc tcgatatcgg tcttgggcgg tagcggcctg ccctcgaaga gtgccgcaag
     1261 gtcgggcctg agcgtcgcac ggggcccgaa gccgccgagc gcgattctga gccccgcgac
     1321 cttgccgccg tcgagcctga acgcgcaggc cgcggtcgcg gtggcgacgt cgcaggccgt
     1381 gcggctatgg cgcagggccg ctaccttgag cccgggctcg agtcgaacct cgacgctcaa
     1441 gataacgccc cgcggcgcgg cgatccacga ttccaggctc gtcgtcgcgg gcgccttgcc
     1501 ccgctcggct atcgtcgccg aggcgccgag cgccagcagg acgggagcga ggctcgagca
     1561 cgacttgttg gcgcccagat tgcccccgac cgtcgcgcgg ttccgcgtgt tgcggttgga
     1621 catggttgcc gcggccgcct taatggcgga cgggatggcc tcggactcga tcagctcctg
     1681 gaaggtcgcg ccggcgccga tgctcagcgc gccgcccttg gcctcgacgg cccgggggag
     1741 gactgacgcc aggtcgacga cgcgctccgg cttgtccctg gcctcgaagg ccagcgcgta
     1801 ggtgccgccg gcgagcgcga gagcgcccgg ccgggcgagc aggtcgagag cctcaccgag
     1861 gctctcgggc ctataatatt cctttaccat gcggcttgta tccccgcttt ctcgtagagg
     1921 cccttggcgc aacggttggc ttcgttacgt atccggtcct cgtcgacgcc gactaagcgc
     1981 ccgttctcca cgaccatctc gccattgacg acgaggtggt cgaccccgtg gttatatcct
     2041 gagaagagta gcgcggccgc cgggtcggac agcgcgccgg cgtactcgag cctcatcacg
     2101 tcgaacagcg cgatgtccgc cagggcgcct atctccatcg agcccgcgtc ctcgtagccg
     2161 agtatgcgcg cgccgccaat cgtcgcgacg ccgagcacct cgcgggccgt aatgccgccc
     2221 gagccgtaac gcacgcgttg caggaggagc gcctgcctgg cctcgccgag catgtcggag
     2281 gcgtcgttcg acgccgagcc gtcgacggcg aggccgacgg cgacgccgcg gtcgagcatc
     2341 tcgcgcacgc ggcaaatgcc ggagccgagc ctcatgttcg atgatgggca gtgcgcgacg
     2401 ccggtgccgg tcctggccag gtagtcgagc tcgtcgtcgt tgaagtgtat gccgtgagcg
     2461 taccagacgt ccttgccgac gaagccgcag tcctccatga ccttgagcgg gcggcggccg
     2521 tacatctgca cgcagaagtc gtcctcgtcg ttcgtctcgg ccaggtgggt atggaggcgg
     2581 gcgccgtacc tgcgcgccag gtcggcggcg tcgcgcatcg actgctcgga gaccgagaac
     2641 ggcgagcacg gcgccagggc gaccttgcgc atcgagtcgg gcgccgcgtc gtggtacttc
     2701 ctgagcgctt cctcgctctg ggcgaggatc tcgtcctcgt cctgtacgac gctgtccggg
     2761 ggcaggccgc cgtccttctt ggagcgcgac atggagccgc gggtcggcgc gaagcgtagg
     2821 cctatgtcgg cggccgcctt gaactggatg ccgggcaggt cgccggtaaa ggagcgcggg
     2881 tacaggtagt ggtggtccgt cgagagcgtg cagccggtct tggccagctc cgccatcgcg
     2941 agcatgctcg accagtagac ggcctcctcg tcgatgccct tccagacttc gtagaggtag
     3001 acgagccagt cgaatagctt ggcgtcctgg acggccggca cgttacgcgt cagcgtctgg
     3061 tagtagtggt ggtgcgtatt gacgagcccg ggcatgacga cgtggcggct cgcgtcgatg
     3121 accttgcagt cgcctgccgg gaccgagagc gctttgccta tcttggcgat gcgcttgccg
     3181 tcgatgagta cgtccacgcc ggacaggtca tccccgacgg aaccggcgtc cgcgccggct
     3241 ccgtagcggg ggcggaccac cctgaagcag tccttgagca gtatcaacgt actacctccg
     3301 cattaaagtc gttgatcatg gcgtcgagat cggcggcctg ggcgcgcagt ccgccccagt
     3361 acgagcgata gtcgctccac tgggcccgga gctcgtccac attcttaccg agctgctcga
     3421 cccagctgaa gtacttgagc tggtgcacgc ggcgcttgtc gacctgagac agctccagca
     3481 cgtagtcgac cgacaggccc tggatcctgt catagtcctt gtcggcctgt cgctggccgt
     3541 aggcgccgcg ctcggcgttc atctcggaga ttcggctacc gtacatggcc atggagtcgg
     3601 tgaacatggt gaagaccacg tcgttctcgt ccatctcgta gtacttggcc atcttgatgg
     3661 ccgcgacgag gttgccgacg cccgagatgc ccaacagatt caggcgggcg atcgtatccg
     3721 caggtacacc catctcggcc agcaccttgt gtccggccgg ctcgttgaac agccggatga
     3781 agcggatggg gagctcgtcg tccacaccga tcgcggcgtc ggtgtcgcga aggttatgga
     3841 tccacggaat gtgcttgtcg ccgatgccct cgatgcggtg gtcgccgaag ccgttctcga
     3901 ggatcgtcgg gcactgcagg gcttccgcga cgccgacctt ggcgccgggg aaggtgtccc
     3961 gaaggtagct gccggagccc atggtcccgg ccgagccgga cgatagcacg gcgccggaga
     4021 cgtggtcgcc gggcttggcg atcttcttga agacctcggc catggccggg ccggtcacgg
     4081 agtagtgcca gaggtggttc gggagctggt cgaactggtt gaagatgacg cactcggggc
     4141 ggttcttctc gatatcggcg caggtgtcga agatctcctt gacgttcgac tcgcacccag
     4201 gcgtggctat cacttcctcg gccatcgtcc tgagccagtc gaaacgctcc ttgctcatgc
     4261 cggcgggcag gatggccacc gaggggcaag acaggagccg ggaaatatag gcgccgccgc
     4321 ggcagtagtt gccggtgctc ggccagacgg ccttcttggt cgtggggtcg aagcggccgg
     4381 tcacgagctc gggtagcagg cacgataggg ccgcgccgac cttgtgggcg ccggtcggga
     4441 accacttgcc gacgaggccg acgatacggg ccttggtgcc ggtgatctcg cgcgggatct
     4501 cgaggtagtt gacctcgccg aactggccgc cctgctcctt gggctcgtta tgccagttga
     4561 cgcggaacag gttgcggcta tggacgtccc agagcccgat gcctttgagc tcgtccttga
     4621 ccgactgggg gatcttggtc gggtccctca tctgctcgta ggtcggaagg ataatgccct
     4681 tttctctgca gcgctggatg ttcttcttgc gcgcctgctc gtctatccgt aaatcgatca
     4741 tgtgtcgctc cttgttagtt gagaaagatt gaaccacgga gacacgaagg cacggagtaa
     4801 tgtaaaagga atgacgtacg aaggaaggag tacacctttc ccatagtatt ttctcctctt
     4861 agctccgtgt ctctgtgcct ccgtggttgt attgttcatt ataatcccag gcccttcatg
     4921 gcgcccgcga cgtccttgag gcccgagccg gtcagcatga cgacgaccgt ctcgtcaggc
     4981 ctgacgccgg ccttggcctt gacgaagccg gcccacgcgg cgctcgagct gggctcggcg
     5041 aagagcccgg ccttggccgc tagctccttc tgggccgcca gtatctccgc gtccgttacg
     5101 acgacggcct tgccgccgtg cgccttgagc ttcgcgagcg cgtgggcgcc gtttcgcggt
     5161 acgtccaccg aaatggagtc cgctaccgtg tcgctcggca cgggctcgcc gaagtccccg
     5221 gcggccaggg cgcgcgcgat ggcgctcgag ccctcggcct ggcaggccca gatcgtcggt
     5281 actttctgga tgcgcccgag cctgaccagg tcctcgaagc ccttgtagac gccggctatg
     5341 atcacgccgt cgccgaccgg cacgaagacg tggtccggcg ccacgtagtc cttcttggcg
     5401 agctcggcga ggtgcccgga gcgcttcgcc tcgtgctccc gcgcgcgggc gaggtcgcgc
     5461 accagctcga agctgacggt cttcttgccc tcgatggtca gtggattgta cgcggtattg
     5521 cgcgacagca cgcccgtctc cctggagtag gcgagcgact ggtcgaacgc cacatcgtag
     5581 gtgccgtcga cctcgcggag ctccgcgccg tactgtagca cctggacgcg cttggcgacc
     5641 ggagccttct tcggaaggaa caccgttatc ttgagtccgg cggcggcgcc gatgcccgac
     5701 atgctcgagg cggcgttgcc ggtgctcgct agcgctatct cggttatgcc gcgttccttg
     5761 gcgaagcccg ccacgaggta cgaggcgcgg tccttgaacg agccggtcgg ctcgcaggtg
     5821 tcgtccttga ggaacagcct cggatagccg gtctccgcac gcagtcggtg cggctcccag
     5881 agcggggtgt ctccgaccgg cagcgggggc aggtgctcct tggctaccgg gagggggata
     5941 tcgtcagggc tgcccgcgaa ggatccttcc caggcgcact cgagtacgcc ctcgagcggt
     6001 cggtcaggcc gttgcctcgc ggcgcacgcg tcgcagaggt agcggccggg ctcgatctcg
     6061 tactccgccc cgcacccggg gcacttataa aaccacctca ccgtatactc cttcgcttaa
     6121 tagagaactt gaaccacgga ggcacgaagg cacagaggta gacaagggga ggatgtaaga
     6181 aaagagatag aaggttgctt cttcatcttc gatgcattca tcatctcttg ctcctcttct
     6241 ttctctttct tctccgtgtc tccgtgtctc cgtggtttta aatttctata acttggcctc
     6301 ttccagcgcg taggggaggg cggcgtagaa ggcggcggcc ttccagaggt cgtccacgcg
     6361 cgtgatctcg ttgggggcgt gggcctggtt ctcgtcgccg gggccgaagc cgatgctcgg
     6421 gataccgtgg cggccggcca ccgctaccgc gttggtcgag aaggtccact tatcgacgac
     6481 cggcgcggcc gtgaagacgg cctcgtgggc ggcgacgccg gccttgacga aggggtgatc
     6541 ggccgggaac ttccaggtcg ggaagtacag ctcctggcgg tagtccttgc cggtccagct
     6601 cgggatggcg tactcgggca tggtcacctc gacggcgtcg acgccggcgg ccaggacggc
     6661 gtccttgacc tgctggatgg ccaggtcctt ggtctcgccc caggtcaggc ggcggtccag
     6721 gtacagcatg gcctcgtccg gcacggcgca ctgcgacggg ccctgcacct tcatctgcga
     6781 cacggtcacc gtgcccttgc ccaggaagtt gtcgtcgtcc ggcttgaggg ccgcgttgag
     6841 cgcctcgatg gcgagggcgg cgcgggcggc cttataggcg gccgagacgc cgcgttcggg
     6901 ggccgaaccg tggcaggaga cacccttcag gtgcacctcc atctccatgc ggccgcggtg
     6961 gccgcgatac aggcggaggc tggtaggctc cgtggagacg aagaagtcag gcacgaactt
     7021 ctcctcctcg atcaggtact tccagcacat gccgtcgcag tcttcctcga tgatggagaa
     7081 gacgaaccag acggaataat cgcctgcgta cttgagctcc ttgaggatgc ggccggcggt
     7141 gatcatcgcg gccgcgccgc cggtctggtc cgaggtgccg cggccgtgga cgaggccgtt
     7201 ctcgatgtcg ccggagaagg ggtccttctt ccactgcgag cgatcgccga tgccgacggt
     7261 gtcgatgtgg gcgtcgatcg cgaggatctt cttgcccgag ccgacgcggg ccaggaggtt
     7321 gccgaggccg tcgatgcgta cctcgtcgaa gccggccttc tcgcacatgc ccttgaggaa
     7381 catgatgcgc tccttctcct tgttgctcat cgcgggaacc ttgatgatgc tcgagaggtt
     7441 ctgcgccgtc cagtccttgt acgcctcggc cttggccctg atttccttac cgttctgcac
     7501 gctcatttcc tttcctccat tcgctgccat aatcgcctgg tctcgtcgcg tccgcgctgc
     7561 gctatggccg cggcgtcgaa taccggcttc ctgtccttga ctatgaatcg tccggccacc
     7621 atcgtgccgg agaccatcct agaggccatg ccgaaggcca cgtgcccggc cacgttgccg
     7681 ctctcgagcg gggtaggcgg gtcgtagtcc cagaacgtca ggtcggcttc gtagccggcc
     7741 tcgacgcggc cgaagcgctt gcccgggaag tatcgctcga gcagcctgtt gccgttctgg
     7801 agcatctcga ggtaggcgtc catccatcgc gtgccgccgg cgtcgtggtg cttgaagaac
     7861 gcgaagcagg cctcctcgag catgtccgag cctatgccgt ccgtgccgag caccgggttg
     7921 cgatactggc cgatcttgtc gttgtagccg acgcagttgt tcatgttcga ccgggcattg
     7981 tgcgctatga acgagtcccg cgcgttgacc aactcgacct cgctcggcgt caggtagagg
     8041 ccgtggccga gtatggtctt ggggccgagg aggcccgcgt cgtcgaggcg cacgcagatg
     8101 tccttgccga agcggtagcg cgagtcgacc gcgtcgaatt tatcctcggc cacgtggatg
     8161 tgcagtccgc gcccggtcga cgagcaggcc gcggccaggc cggagagcgt ctcgtcgtcc
     8221 acggtgaacg gcgcgtgggc gccgatggcc gcttcgatga gcgggtctcc tccggccttg
     8281 cgctcgtcgt cgacgcggcg ggcgaagcgc tcgttctcgc ggatgcccgc ctgagctcct
     8341 tcgcggccgt tgcggtcggt cgtctcgtag catagcacgc cgcgtagacc gaccgtctcg
     8401 aagccctcgg ccagagcgtc aagggagccg tcgatgaact cggggctggc gtgatggtcg
     8461 acgaccgagg taacgccgag catcgccgcg tccgcggccc cggccaggcc ggaagcccgc
     8521 gtgatgccct cgtcgatggc gcggtccagg agccaccaca tgttcttgag ctgctgcgcg
     8581 aagtccttgc tcggctggat ggcgaccatg agtccgcgcg acagggccga gtagaagtgg
     8641 ttgtgggagc agacgaggcc cgggctgacg tagccgccgg gagacaggcg ctcggcgccc
     8701 ggatgcctgg cggcgaggcc gcggcccacc tcgacgatct
//
LOCUS       DAOPHV010000036         8715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_10135_length_8715_cov_3.837413, whole genome shotgun sequence.
ACCESSION   DAOPHV010000036 DAOPHV010000000
VERSION     DAOPHV010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10135_length_8715_cov_3.837413"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /gene="lnt"
                     /locus_tag="PLQ29_01770"
     CDS             complement(<1..1233)
                     /gene="lnt"
                     /locus_tag="PLQ29_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPG85400.1"
                     /translation="MGIARRLFLGLISSLLLALSLPNELFSWGAPALGLVALAPLFVA
                     LAESGSYRESFAIGSTFGGLAHGLSSYWLWFFKDFRFWTLGTTVIAYMIVYGTLGLYL
                     RGTLKRGGLARPLIFAMAWAVFEWGKSSGFLGYPWGLVAYSWNTVLPAVQIAESTGVY
                     GLSFALAFVSASIGEVLCGPPARLPGPTDGVAERTPAGRLSAIVPPRPAWPDGRYASL
                     GNLVVAVLCLVAIYAYGLVALERPREARGTVDVLLVQQNVDPWEGTEAENLAGSIALA
                     SKALDSAGAGADLVLFSESTLRRPWNGFQRYFSRTPAGYPLGSFIRDSGAHFLTGAPE
                     ILDYESFEATNSVILISPEGRQVGSYAKTHPVPFAEAIPFWEVPAVRAFIQDVVGLAS
                     GWTMGVERVLFDLPTARSG"
     gene            1343..1837
                     /gene="coaD"
                     /locus_tag="PLQ29_01775"
     CDS             1343..1837
                     /gene="coaD"
                     /locus_tag="PLQ29_01775"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPG85401.1"
                     /translation="MVKAVFPGSFDPPTFGHLNIIERARTIFERVLVVVAVNRDKRYL
                     FDADERMAFMKELVAPWPNVEVHRCDTLIVDFAREHACRVLLRGVRSVQDFSYEFELS
                     ILNKGVGPEIETVFMPTDQKFFVLRSSAIKELASFGGDVSAMVPPVVAKALERFRVGA
                     HSST"
     gene            1910..2098
                     /gene="rpmF"
                     /locus_tag="PLQ29_01780"
     CDS             1910..2098
                     /gene="rpmF"
                     /locus_tag="PLQ29_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPG85402.1"
                     /translation="MAVPRCNTSKARSRRRRSINMKLVAPTIVSCGNCGNAVLFHRVC
                     PKCGFYRGRQVLTPETNA"
     gene            2111..2344
                     /gene="acpP"
                     /locus_tag="PLQ29_01785"
     CDS             2111..2344
                     /gene="acpP"
                     /locus_tag="PLQ29_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPG85403.1"
                     /translation="MDELYEKIKKIIADKLEVEESKINLDASFRQDLGADSLDTYELV
                     YGIEEELKIRIPDEKANEFETVRDAYEFIKSQV"
     gene            2402..3169
                     /gene="rnc"
                     /locus_tag="PLQ29_01790"
     CDS             2402..3169
                     /gene="rnc"
                     /locus_tag="PLQ29_01790"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPG85404.1"
                     /translation="MSFRYDDIGRDRLEALAAFQRSLRFRFKDLSLLNRALTHRSHVN
                     EDPASRGNNERLEFLGDAVLGMATASRLFIALGDKSEGDLARIKSFVVSEDTLSDIAS
                     RLGVDGVLLVGKGEERSGGRNKKAILADAMEAVFGAAYLDAGFDKAKDLVLSLIDPEI
                     TKVLTDRHRKDYKTLIQEYAQKWHRCYPVYTLERKAGPDHDRTYWIRCELLGASYGPA
                     AGKTKKDAEQASAALAYEAILGRGDAETERLLATKRL"
     gene            complement(3182..4540)
                     /locus_tag="PLQ29_01795"
     CDS             complement(3182..4540)
                     /locus_tag="PLQ29_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HPG85405.1"
                     /translation="MKRRLSVPKDLREFAAVFAKAGHSCYFVGGAVRDGLLGRPVSDW
                     DAATDATPERVQALFRSVIPTGVQHGTVTVRWRGASIETTTFRVDGEYRDGRRPESVA
                     FTGDLLADLSRRDFTINGMAVDPATGEVVDPAGGMADLEAGVVRAIGDPIARFTEDGL
                     RPMRAVRFAARFGFSIDPATFAAIPATLCRFRLVSAERVRDEFSKILTCDRPSDGLRS
                     LYDSGLLAEFLPELAACAGVGQGGQHRHDVLGHSIAACQAAPANLELRLAALLHDVGK
                     PTRRVESADGSLSFHGHELESARLAAAALRRLKYPNAVVDAVSHLVKHHMFDYHDSWT
                     DAAVRRFVSRVGLDWVKPLAILRLADSGGMGAGPADPRSVMPLLERVEALVAKDQAFG
                     LKDLAIGGDELAAAGWPKGPVMGRVLAELLEAVLDDPELNTRERLLDIAGRIKAKYGV
                     GD"
     gene            complement(4553..5569)
                     /gene="queA"
                     /locus_tag="PLQ29_01800"
     CDS             complement(4553..5569)
                     /gene="queA"
                     /locus_tag="PLQ29_01800"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPG85406.1"
                     /translation="MRTTDFSFNLPEVLIAQTPSERRGESRLLVLDRGSGELTHSAVR
                     TIAEFVPSGAVMVFNDSRVRRARLFASTPNGGRVEVLLLRREGPALWAALTTRMAKLT
                     PGKRLELPEGVEAEVVEALADERVFRLSREIDDDYLERNGHLPLPPYIRREDAPTDAE
                     RYQTVYARERGSSAAPTAGLHFTEGILSGLEAAGVVLRFVTLHVGLGTFLPVRSESIE
                     DHRMPREEYSIPAATADAVNAAKRDGRPVVAVGTTSVRTLESAWTEEGLPAAAGSTNI
                     FIYPGYRFKAVDMLFTNFHTPESTLLMLVSAFAGRERILSAYQEAVRERYRFFSYGDA
                     MLIR"
     gene            complement(5544..6560)
                     /gene="ruvB"
                     /locus_tag="PLQ29_01805"
     CDS             complement(5544..6560)
                     /gene="ruvB"
                     /locus_tag="PLQ29_01805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPG85407.1"
                     /translation="MSALVSGSFDPAEDERENALRPRRLTEFLGQASVKANLSVFIEA
                     ALKRSEALDHVFLMGPPGLGKTTLAGILSHELGVDFKVTSAPALEKPKDLAGILTTLA
                     PRSIFFIDEIHRLKPAIEEMLYMAMEDGELDFIIGQGPAARTLRIPLPPFTLVGATTR
                     AGMVGSPLVSRFGISVRLGFYDRADTVAILRRSAGILGMSLDDDAAVRLAEASRGTPR
                     VANRLLRRMRDFAQVAGSDAITEDVVRDGLERLEVDRYGLERHDRDVLAAIIEKFGGG
                     PVGAETLAVSTSESVETLEDYLEPYLIQSGFIMRTPRGRVATPLAYEHLGYRNDHAND
                     GFQF"
     gene            complement(6557..7150)
                     /gene="ruvA"
                     /locus_tag="PLQ29_01810"
     CDS             complement(6557..7150)
                     /gene="ruvA"
                     /locus_tag="PLQ29_01810"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPG85408.1"
                     /translation="MFNAIRGTLCHKGADTIRIDANGVEWEFSVPARSVDAFGRLGDE
                     ARVLCWLLHREDSMRLYGFPSEAERSIFIELIGVDGIGPKQALKILGGIGAGDLERAL
                     ESEDLARLESVPGLGKKTAQKLVFALKGRLPSVSGASEAAPAGPHGDIVRALVDMGYD
                     RRRVAEALARVDAGIPPDHPERERELFRLAIVELSAS"
     gene            complement(7158..7691)
                     /gene="ruvC"
                     /locus_tag="PLQ29_01815"
     CDS             complement(7158..7691)
                     /gene="ruvC"
                     /locus_tag="PLQ29_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPG85409.1"
                     /translation="MPGGTERLRVLGIDPGLAAVGWGVVDLDGARVVYRGHGCFVTKP
                     GVGMAERLALIRDALKDVIAEYGPSEAAMEALYFSRNVTSALAVAEARGVIRLACRDA
                     GLSMSEYGPMDVKKAATGSGRSGKDDVQSFVRLVLGLSAIPKPDHAADALAVALCHCQ
                     GAGVRSALVDASRPGRL"
     gene            complement(7695..8432)
                     /locus_tag="PLQ29_01820"
     CDS             complement(7695..8432)
                     /locus_tag="PLQ29_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPG85410.1"
                     /translation="MSGHSKWATIKHKKGAADAKRGQMFTKLIKEISIAARMGGGDPD
                     TNARLRTAVLKARGANMPKDNIDRAIKKGTGELGAAVYEELVYEAYAPGGIAILIEVL
                     TDNKNRAAAEVRNILTRSGGQLATSNAVLRLFARKGVISLDGERYSEDKVMELALEAG
                     ADDIVNEDGELVVYTSPEAFEAVLNAVNAAGMETDGAEISMIPETYVTPEGDNGGKIQ
                     KLIDKLEESDDVQNVYHNAELPEEAEE"
BASE COUNT         1514 a         2812 c         2931 g         1458 t
ORIGIN      
        1 ccccgaccgg gccgtgggca ggtcgaacag cacgcgttcc acgcccatcg tccaacccga
       61 cgccaggcct acgacgtctt ggatgaacgc gcggacggcc ggaacctccc agaacggtat
      121 ggcctcggcg aagggcacgg ggtgggtctt ggcgtaggag ccgacctgac gaccctcggg
      181 gcttatcaga atgaccgagt tcgtagcctc gaagctctca tagtcgagaa tctctggcgc
      241 gccggtcagg aaatgggctc ccgagtctcg gatgaagctc ccaagcggat agccggccgg
      301 cgtgcgcgag aagtagcgct ggaaaccgtt ccagggccgg cgtagcgtcg actcgctgaa
      361 cagcacgagg tcggcgcccg cgccggcaga gtcgagcgcc ttcgaagcca gggcgatgga
      421 gccggccagg ttctcggcct cggtgccttc ccaggggtct acgttctgct gcaccaggag
      481 gacgtcgacc gtcccgcggg cctccctcgg ccgctcgagc gctaccaggc cgtaggcgta
      541 gatagccacg aggcatagga ctgcgacgac gaggttgcct aggctagcgt agcgaccgtc
      601 cggccaggcc ggccgaggtg gtacgatggc ggagaggcga cccgccggcg tccgctcggc
      661 tacgccatca gtcgggccag gcaagcgcgc gggggggccg cagagaacct cgccgatcga
      721 ggccgagacg aaggccaacg cgaacgacag tccgtacacg ccggttgact cggcaatctg
      781 cacggcggga agcaccgtgt tccagctata ggcgacgagc ccccatgggt agccgagaaa
      841 gccggacgac ttgccccact cgaagacagc ccaggccatc gcgaagatga gcggccgcgc
      901 cagcccacct cgcttgagtg tcccgcgcag gtatagcccc agggtcccgt agactatcat
      961 gtaggcgatc accgtcgtgc cgagcgtcca gaacctgaag tccttgaaga accatagcca
     1021 gtagctggac aggccgtggg ccaggccgcc gaacgtagat cctatggcga acgactcgcg
     1081 gtacgacccg gattccgcca gggcgacgaa cagcggcgcg agcgccacga ggccaagggc
     1141 cggggcgccc catgagaaca gctcgttggg gagcgatagt gccaggagga ggctcgagat
     1201 caagccgagg aagagtcgcc gcgcgatgcc catgggcaca tgatacacgc tgatcgagcc
     1261 cagcgccagc gatggccgtc ggcgggcgcg tatacgtgga aaacaccttg acggcgctcg
     1321 cctacccgga tatggttcgt ccatggtcaa ggccgtcttt cccggttcgt tcgatccccc
     1381 gacgttcggg cacctgaaca tcatcgagcg cgcccgtacg atattcgagc gggtcctggt
     1441 ggtcgtcgcc gtcaaccgcg acaagcgcta tctgttcgac gccgacgagc gcatggcgtt
     1501 catgaaagag ctggtcgcgc cctggccgaa cgtcgaggtg caccgctgcg acaccctgat
     1561 agtcgatttc gcgcgggagc acgcctgcag ggtgctattg cgcggcgtac ggagcgttca
     1621 ggacttctcg tacgagttcg agttgtctat cctgaataag ggagtcggcc cggagatcga
     1681 gacggtcttc atgcctacgg atcagaaatt cttcgtactg cgctcgagcg ccatcaagga
     1741 gctcgcctcc ttcggcggcg acgtcagcgc catggtgccg ccggtcgtgg ccaaggcgct
     1801 cgagcgtttc agggtcggcg cccactcctc gacttgacaa atatccggta tttcgttatt
     1861 gtgcgccggt atccgttaca ggcacaattt gtgggaatga ggttatagta tggctgtacc
     1921 cagatgtaat acttcgaagg cacgttcgcg caggcgccgc tctatcaata tgaagctcgt
     1981 cgcgcccacg atcgtttcct gcgggaactg cggcaacgcc gtactgttcc acagggtttg
     2041 cccgaagtgc ggcttctatc gcggcaggca ggttttgacc cccgaaacca acgcgtaaag
     2101 gagactcgcc atggacgagt tgtatgagaa gatcaagaag ataatcgccg acaagctcga
     2161 ggtcgaagag tcaaagatca acctcgacgc cagctttagg caggatcttg gcgccgacag
     2221 cctcgatacc tacgagctgg tctacggcat tgaggaagag ctgaagatcc gcatccccga
     2281 cgagaaggcc aacgagttcg agaccgtccg ggacgcgtac gaatttatca agtcccaggt
     2341 gtgacgtagc cgggcgggct tgctggcccg tccgcaatcg tcggtcaagg aggccgcgat
     2401 cgtgtccttc cgttacgacg acattggtcg tgatcggctg gaggcgctcg cggcctttca
     2461 gcgttctttg cggttccgct tcaaggatct atcgctcctc aaccgggcgc tgacccaccg
     2521 ctcccacgtc aacgaggacc ccgcgtcgcg aggtaacaac gagcggctcg aattcctcgg
     2581 cgatgccgtg ctgggcatgg ctaccgcctc gcgccttttc atcgccctcg gcgacaagtc
     2641 ggagggcgac ctcgccagga tcaagagctt cgtcgtctca gaggataccc tatcggacat
     2701 agcctcgagg ctaggggtcg atggggtgtt gctcgtaggc aagggcgagg aacgctcggg
     2761 cgggcgcaac aagaaggcca tcctggccga cgcgatggag gccgtcttcg gcgcggccta
     2821 cctcgacgcc ggcttcgata aggccaagga cctcgtactg tcgctcatcg atcccgagat
     2881 aaccaaggtc ctgaccgata ggcaccgcaa ggactacaag acgctgatcc aggagtacgc
     2941 ccagaagtgg caccgttgct acccggtcta tacgctcgag cggaaggccg gaccggacca
     3001 cgaccgcacc tattggatac gctgcgagtt gctcggcgcg agctatggcc cggccgcggg
     3061 caagaccaag aaggacgccg agcaggcctc ggccgccctg gcctatgagg cgatcctcgg
     3121 gcgcggagac gccgagacgg agcgcctcct agcgacgaag agactctagc gccgtccggc
     3181 cctagtcccc taccccgtac ttagccttga tcctaccggc tatatcgagc agacgctccc
     3241 tggtattcag ttccgggtcg tcgagcaccg cctcgagcag ctcggcgagg acccggccca
     3301 tgaccgggcc tttcggccag ccggccgccg ccagctcgtc gccgcctatg gccaggtcct
     3361 tgaggccgaa ggcctgatcc ttggcgacga gagcttccac ccgctctagg agcggcatca
     3421 ccgagcgcgg gtccgccggg cccgcgccca tgccgccgga gtcggccagc ctgaggattg
     3481 cgagcggctt gacccagtcc agccccaccc gcgagacgaa gcgcctgacg gccgcgtcgg
     3541 tccacgagtc gtgataatcg aacatatggt gcttgacgag gtgcgagacc gcgtcgacga
     3601 ccgcgttcgg atacttgagc cgcctcaggg ccgccgccgc gagcctggcc gactcgagct
     3661 cgtgcccgtg aaacgatagg gagccgtccg cggactctac ccgtctggtc ggctttccga
     3721 cgtcgtgtag cagggccgcg agcctgagct ccaggttggc cggggcggcc tggcaggcgg
     3781 cgatcgagtg tccgagcacg tcgtggcgat gctggccgcc ttgcccgaca ccggcgcagg
     3841 cggccagctc cggtaggaac tcggcgagga ggccggagtc gtatagcgat cggagcccat
     3901 cgcttggccg gtcgcaggtc aggatcttcg agaactcatc gcgcacccgc tcggctgata
     3961 cgagcctgaa ccggcaaagc gtcgccggaa tggccgcgaa ggtggccggg tcgatcgaga
     4021 agccaaacct ggcggcgaag cgtacggcgc gcataggccg gagaccgtcc tcggtgaagc
     4081 gggctatagg gtcgccgatg gcgcgaacga cgccggcctc caggtcggcc atgcctccgg
     4141 ccgggtcgac gacctcgccc gtggccggat cgacggccat gccgttgatg gtgaaatcgc
     4201 ggcgggacag gtccgcgagc aggtcaccgg tgaaggcgac ggactcgggg cggcggccgt
     4261 cgcggtactc gccgtcgacg cggaaggtag tcgtctcgat cgaagcgccg cgccagcgca
     4321 ccgtgacggt gccgtgttgg acgccggtcg gtatgaccga ccgaaacaag gcctggaccc
     4381 gctcgggtgt ggcgtcggtg gcggcgtccc agtcgctcac gggcctaccg aggaggccgt
     4441 cgcgcacggc gccgccgacg aagtagcacg agtggccggc tttcgcgaag acggcggcga
     4501 actccctgag atccttgggt acggatagtc gtcgcttcat ggcgctggac ccttagcgta
     4561 tgagcatggc gtcgccgtag gagaagaagc ggtaacgttc ccgcacggct tcctggtaag
     4621 ccgatagaat gcgttcgcga ccggcgaagg cggagacaag catcaagagg gtcgactcgg
     4681 gggtatggaa gttggtgaac agcatatcga cggccttgaa gcggtagccg gggtagatga
     4741 agatgttcgt cgagccagcg gcggccggca acccctcttc ggtccacgcg ctctcgagcg
     4801 tgcggacgct cgtggtgccg acggcgacga cggggcgccc atcccgcttg gcggcgttga
     4861 ccgcgtccgc cgtggcggcc ggaatcgagt actcctcgcg gggcatgcgg tggtcctcga
     4921 tcgactcgga gcgcacgggc aggaaggtcc cgagtccgac gtgcagggtg acgaagcgca
     4981 acacgacccc ggcggcctcg agccctgaca agatcccctc ggtgaagtgt agcccggcgg
     5041 tcggggcggc cgacgaaccg cgctcgcgcg cgtagacggt ctggtagcgc tccgcgtcgg
     5101 tcggcgcgtc ttcccgcctg atatagggcg gtaggggcag gtgcccgttg cgctcgaggt
     5161 agtcgtcgtc gatctccctc gacaagcgga agacccgttc gtcggccagg gcctcgacca
     5221 cctcggcctc gacaccctcg ggcagctcga gccgcttgcc cggcgtcaac ttggccatcc
     5281 gcgtcgtcag cgcggcccag agggccgggc cttcgcgcct gaggaggagc acctcgaccc
     5341 tgccgccgtt cggcgtcgac gcgaagagcc tggcccgcct gacgcgtgag tcgttgaata
     5401 ccatcaccgc tccggagggc acgaactcgg caatagtacg caccgcggag tgcgtcagtt
     5461 ccccggagcc gcggtcgagc acgaggagcc gcgactcgcc gcgccgctcg ctcggcgtct
     5521 gcgcgatgag tacctcgggt aggttaaaac tgaaatccgt cgttcgcatg gtcattcctg
     5581 tagcctaggt gctcgtaggc gagcggcgtg gccaccctgc cccgtggggt acgcatgatg
     5641 aagcccgatt ggatgaggta cggctcaagg tagtcctcga gcgtctcgac gctctcgctc
     5701 gtcgagacgg ccagggtctc ggcgccgacc ggtccgccgc cgaatttctc gatgatcgcg
     5761 gcgagcacgt cgcggtcgtg gcgctcgagc ccgtagcggt ccacctcgag ccgctcgaga
     5821 ccgtcgcgga cgacgtcctc ggtgatagcg tctgagccgg ccacctgggc gaagtcgcgc
     5881 atgcggcgga gcaggcggtt ggcgacgcgg ggcgtgccgc ggctggcctc ggccagcctg
     5941 acggcggcgt cgtcgtcgag actcataccg aggatgcctg ccgagcgccg tagaatggcc
     6001 acggtgtccg cccggtcgta gaagccgagg cgcacgctga tgccgaaacg gctgacgagg
     6061 gggctcccga ccatgccggc gcgtgtcgtc gccccgacga gcgtgaacgg gggcagcggg
     6121 atgcgcagag tcctggccgc cggtccctgg ccgatgatga agtcgagctc gccgtcctcc
     6181 atcgccatgt acagcatctc ctcgatcgcc ggtttgagcc tatggatctc gtcgatgaag
     6241 aagatcgatc tgggcgcgag ggtcgtcagt atgccggcca ggtccttggg cttctcgagt
     6301 gccggggcgc tcgttacctt gaagtcgacg ccgagctcgt gcgacaggat gccggcgagc
     6361 gtcgtcttgc cgaggccggg cggccccatc aagaacacgt ggtcgagcgc ctcgctgcgc
     6421 ttcagggccg cctcgatgaa gaccgacagg ttggccttga ccgaggcctg cccgaggaac
     6481 tcggtcaggc gccggggcct gagcgcgttc tcccgttcgt cttcggccgg gtcgaacgag
     6541 ccggatacca gggcgctcac gaggcgctca gctcgacgat cgcgagcctg aacagctccc
     6601 gttcgcgctc gggatggtcg gggggaatgc cggcgtcgac gcgggccagc gcctcggcga
     6661 cccggcgcct atcgtagccc atgtcgacga gggcccgcac gatgtcgcca tgcggcccgg
     6721 cgggggcggc ctccgatgct cccgataccg acggcaacct gcccttgagg gcgaagacca
     6781 gcttctgggc cgtcttcttg ccaagtccgg ggacggactc gagcctggcc aggtcttcgc
     6841 tctcgagcgc gcgctcgagg tcgccagccc cgatgccgcc aagtatcttg agggcctgct
     6901 tcggcccgat gccgtcgacg cctatcagct cgatgaagat cgagcgctcc gcctcggagg
     6961 ggaagccata gaggcgcatc gaatcctcgc ggtggagtag ccagcataga acgcgcgcct
     7021 cgtcgccgag ccgcccgaag gcgtcgaccg agcgagccgg caccgagaat tcccactcga
     7081 cgccattggc gtctatcctg atcgtgtcgg cgcccttatg gcagagcgtg ccgcgaatag
     7141 cgttgaacat agccggatta tagtcggccc gggcgcgatg catcaaccaa ggccgagcgc
     7201 acgccggctc cctggcaatg gcataacgcc accgcgagcg cgtcggcggc gtggtcgggc
     7261 ttcgggatgg ccgacaggcc gagcaccagc cggacgaagg actggacgtc gtccttgccc
     7321 gagcgccccg agccggtcgc cgccttcttg acgtccatcg ggccgtactc gctcatggaa
     7381 aggccggcat cgcggcaggc gagccgtatc acgcctcgag cctccgcgac ggcgagcgcg
     7441 cttgtcacgt tgcgtgagaa gtatagggcc tccatcgcgg cctccgacgg cccgtactcg
     7501 gcgatcacgt ccttgagcgc atccctgatc aacgccaggc gctcggccat ccccacgccc
     7561 ggcttggtta cgaagcagcc gtgtcccctg tatacgacgc gggcgccgtc taggtcgacg
     7621 acgccccatc ccacggccgc gaggccgggg tctatgccga ggacgcggag cctttcggtt
     7681 ccgcccggca tggactactc ctcggcttcc tcagggagct cagcgttatg gtagacgttc
     7741 tgtacgtcgt cggattcctc gagcttgtcg atgagcttct ggatcttgcc cccgttatcg
     7801 ccctcgggcg taacgtaggt ctccggtatc atggatatct cagcgccgtc ggtttccatg
     7861 ccggccgcgt tgaccgcgtt cagcacggcc tcgaaggcct ccgggctggt atagacgacg
     7921 agttcaccgt cctcgttgac gatgtcgtcg gcgccggcct cgagggcgag ctccatgacc
     7981 ttgtcctcgc tgtaccgctc cccgtcgagc gatatgacgc ccttgcgggc gaacagccgg
     8041 agtaccgcgt tggaggtggc cagctggccg ccggaacggg tcaggatatt gcgcacctcg
     8101 gcggcagcgc gattcttatt gtcggtcagg acttcgatca ggatggcgat gccgccgggg
     8161 gcgtaggcct cgtagaccag ctcctcgtag accgcggccc cgagttcgcc ggtacctttc
     8221 ttgatggccc ggtcgatgtt gtccttgggc atgttagcgc cgcgggcctt gaggacggcg
     8281 gtcctgaggc gggcgttggt atcgggatcg ccgccgccca tgcgcgccgc gatcgagatt
     8341 tccttgatca gcttggtgaa catctggccg cgcttagcgt cggccgcgcc cttcttgtgt
     8401 ttgatcgtcg cccatttgct atgtcctgac attgtgactc cttgccgtgt agaatacgcg
     8461 gttgcgaacc gatcgacgat acgcgcttcc gaggctggaa agcctatgaa aacgggatga
     8521 tccgttcatt ttttgcgagc gtaatactag cacaagccgc gatttactgt caacgattgg
     8581 actatatagc gtaaaaggag ctaaaagcgc agaaaacggc agaacaaact cggctacaag
     8641 ccacggcctc gcggagagcg gaaccgtggc caaggtcgtc gactcagcgc tgagtcgacg
     8701 accttggcca cggtt
//
LOCUS       DAOPHV010000037         8684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_10183_length_8684_cov_4.011821, whole genome shotgun sequence.
ACCESSION   DAOPHV010000037 DAOPHV010000000
VERSION     DAOPHV010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8684
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10183_length_8684_cov_4.011821"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PLQ29_01825"
     CDS             complement(<1..162)
                     /locus_tag="PLQ29_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG85411.1"
                     /translation="MAKVILKDVTKIYDGDVKAVDSANIVIEDKEFVVFVGPSGCGKS
                     TTLRMVAGLE"
     regulatory      283..410
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            406..1875
                     /locus_tag="PLQ29_01830"
     CDS             406..1875
                     /locus_tag="PLQ29_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPG85412.1"
                     /translation="MSRAIALLFTLLSLRLAAYASPLELTYNGASLRLPADGARPMPT
                     GVPGERGYSLGELFPPQLEAWSLDLRSAIGPETMAADDLGERLFDMYAVGTDRGWDLV
                     IGTGQPGQPRRVAGVTAIALRGEPCPERTIEVWVSWEGVPELKAEIRRWAERAGVDAK
                     VVDVPSIKSKLITVLRGGGKAPDLVMVQSDYLPDLQEAGALQPLDGLRLPASAVKGRQ
                     SFELSGRLLAAPFYCDTQLVFYSTGAIREAPRADWTLADMERLAKASGARVGAAWNAY
                     SAYWFLPFVIGYGKESVVGPDGGMNVRHPAYGAALTYLRDSAARGFLTPMERDAMMAY
                     FTSGKAAFILSGSYSIPEFKRLGIPFGVAPFPLVSVDGKRLAPMLDYKGFAVTRTTKS
                     PVLARRLVQYLSSAGVQAAFCGPQGKLPADERAWSLLPADDPYQATIRASWEAGAAVP
                     PDPVYGDFKNASWKLIRLYLGGSTTLGETMEALARIMGE"
     gene            1923..3449
                     /locus_tag="PLQ29_01835"
     CDS             1923..3449
                     /locus_tag="PLQ29_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPG85413.1"
                     /translation="MKRTIWMGGTALVAALALAVLAACVSQDKAVAQEEVPVDATSAA
                     TDGTSGATEAASSSGGAGAKGDRSFQDGSGWAVSLKGIREATLWQSYYEEAAKHASHY
                     VEKKVDKKGVMTSYRGMPLRLAIAMIDGAEKDHAWLFDAALWAAGYDVTLVASDGYSV
                     TFNTKDVAADALIIADREDGTKIPPMVVGDAAKNLWVRDLAMIETSLAPSALVAAAAS
                     FSLDLDINGAKASWSLAELQRLPIYTEGRGAYTTSAGTRYEGVYGGVRLLDLLKTYAD
                     IGADDSVTFVAMDGYEMSYPGKTVLDQADGEWLLAFRLDGDWLPKDPGYIRTIKVGPK
                     TPNIDGHLSVRMIKKIVVKQKDYVDFTLSLSGKMRFDLDRGTVQSCVSCHTRTVTFER
                     KGVVAEYTGFPLWLLLGYVDDPEYAPHKQDTSIKTYMDDAADAGYAIKLSAIDGYSII
                     LDSRDITRNDDIIVAMYRGGEKLPPEESPLVLVWDRAAKKIPEGIKNVKMLASVGATF
                     "
     gene            3551..5617
                     /locus_tag="PLQ29_01840"
     CDS             3551..5617
                     /locus_tag="PLQ29_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPG85414.1"
                     /translation="MALVVLAGAAPFLSALRESFLSGGAYIGLANYRELFADRGFPMA
                     AAITIGWSLLSACAVMVLAYPIASLALGSRRAYAIIFPLLVAMWSVPVYIGAPLWRFV
                     LHGAAGDSVFRALTGIEVNLMESPVAAFVSSALVAAWFRLPQAVFIMLAALGRSRRST
                     DDAARIDGAGPAALAFTVRLPAMAGAIGAVAALEIVSAFKEFTVPFLMTAGGPPLRAG
                     ITERTVVGATTTLELYLYDLFSGYADSGVVSAYAVCLSLVVGAAVAGGFAVRAALKRR
                     SPLSRAESRTVGPAPSPDASPSPRAESRTVGPAPSPDASPSPDASPSPDASPSPRAES
                     RTVGAAVAGGFAVRAALKRRSPLSRAESRTVGPAPSPDASPSPDAVADAGSSPSDLGG
                     SAPSLGGARAPRRIGRVPGRLADAAFAGSSWLVVAVLVAAAAVLAFCILWMAFSDLSI
                     AFIDSPIPRFFTTANFGKAIFDDGLWLALANTVYVSALTAALVGVVAFPAAAWLADRP
                     RARAAAFFVALQALSSAGGVHSLIPLYDLWRRLGLLGGYAPVVLVYLYHSLPVALFAL
                     AEFMRDQPPSFKDAARLEGLGTLGYLWRVQLPLALPAIGAAAMVAFLSAWNGFMAPLV
                     FLDDDATYTIAVKLHAYVGSIASGSPKWNLFAAASIVNVAIIGLLFRRFKKPLATSAL
                     SDQIDD"
     gene            complement(5614..6456)
                     /locus_tag="PLQ29_01845"
     CDS             complement(5614..6456)
                     /locus_tag="PLQ29_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HPG85415.1"
                     /translation="MIAVRKLMTLSPLHRARKAAQVLGHLEADYRQGRTIAGAWIAEL
                     GAAFAADGSLSAELRALAAEASSEAGKSALSSPEAGVRLINKLSRGLEAHAGLSPADW
                     DLREPLGSSLAVSERRVFPGLRAYLEDLRSPFNVGSIFRSADAFGFAELLLSGFTADP
                     AHPRAARSAMGAVEAVGWRRAGLEALEAEREAGGAVLALELRGESVDGFAFPERGIVV
                     VGSEELGVSPEAMALCTATVSIPMLGAKGSLNAGVAFGVLANAWRSSLAVRGVEPVRY
                     SANR"
     gene            complement(6648..7808)
                     /locus_tag="PLQ29_01850"
     CDS             complement(6648..7808)
                     /locus_tag="PLQ29_01850"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPG85416.1"
                     /translation="MADFRFHVPADCYIGQDALYKLPLVLDGGPERALLVADPGLAEA
                     KTAERVAAILEGRGIQLITFDDLAERPSSAAVEEAASLARGARVPLVIGLGGVRAMHV
                     AKAVAALAPAGSSGERGVDAWIDGEPPVRPPLPLALVPTSYRDPFLLSGGLVLTDART
                     GGAVFLQAKRGIETAVVLDPNAFGGLSAKSSAEALLDGAMIAIEGYASAREGFLSDMT
                     LKEAAGLYIRALDATLQRPEDPQARMDAARACFLAGLGLAASSPGLGTAISFAVNARW
                     GVPKANLAAVILPYLLESLSRSRPEKIASLAPLFCEIEPDEGAAGAAARAVESIRTRL
                     GQLKIPSRLKDFELPLERLVETAESARKFDFMNFLPRTMTVDDVFDFIKTVY"
     gene            complement(7923..8201)
                     /locus_tag="PLQ29_01855"
     CDS             complement(7923..8201)
                     /locus_tag="PLQ29_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HPG85417.1"
                     /translation="MTIDRLNPLDPIQPKKPSGAARAERNSGSDSVSLSSEAVEKAEL
                     FKALELAKAAPEIRMDRVAELKAKIDDPDYLNDAILSATADRILDQLL"
     gene            complement(8254..>8684)
                     /locus_tag="PLQ29_01860"
     CDS             complement(8254..>8684)
                     /locus_tag="PLQ29_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPG85418.1"
                     /translation="TPCLSDPGILAAARAREAGHDVVPIPGPSAFAALVSASGIFYKT
                     VAFDGFPSPKPGRRRSRVAELMSRDEAFVMYESPHRIVKLLTDIADIDAERRLCVGRE
                     MTKLHEEFLVGSAAEVRDLLAARPEVKGEIALLVSGAKKG"
BASE COUNT         1357 a         3074 c         2859 g         1394 t
ORIGIN      
        1 ctccaagccg gcgaccatgc ggagcgtcgt cgacttgccg cagcccgacg ggccgacgaa
       61 gacgacgaat tccttgtcct cgatcacgat gttggcggaa tcgaccgcct ttacgtcacc
      121 gtcgtagatc ttggtgacgt ccttgagaat taccttggcc attggccctc ccctgagtga
      181 ggctatatta tgcggtcagt gtacgggcgt tccggcgaat tgtcaaacga aggctcgtgt
      241 tccgccttga caataccccg aagggcccgg agaatgggcc ggtatcgttc cttcgagccc
      301 cctcgcctac cggcgcggcc acatagccgc ggcccacggc gtccgggcca aagggccggc
      361 ggtggaaacg ccccgacctt ccgcgcttga aaaggaggac gcgaggtgag ccgcgctata
      421 gccctgctct tcacgctact atcgcttcgc ctcgctgcgt acgcgtcgcc gctcgaactt
      481 acgtataacg gagcctcgct ccggctcccg gccgacgggg cgcgcccgat gcccacaggc
      541 gttcccgggg agcgaggcta cagcctcggc gaactgttcc cgccgcagct cgaggcctgg
      601 agtctcgacc tgcgttcggc gatcggcccc gagaccatgg cggccgacga cctcggcgag
      661 cgcctcttcg acatgtacgc cgtcgggacg gaccgcggct gggacctcgt gatcggaacc
      721 ggccaaccgg gtcagccccg gcgcgtagcg ggcgtcaccg ccatcgccct gcgcggcgag
      781 ccctgtccag agcgcaccat cgaggtttgg gtgtcgtggg aaggcgttcc cgagctcaaa
      841 gccgagatac ggcgctgggc cgagcgcgct ggcgtcgacg ccaaggtcgt cgatgtgccg
      901 tcgatcaaga gcaagctcat caccgtgctc cgcggaggcg gcaaggcccc cgacctcgtc
      961 atggtccagt ccgactacct acccgacctc caggaggccg gggccctgca gcccctggac
     1021 ggactcaggc taccggctag cgccgtcaaa ggccggcagt ccttcgagct ttcaggcagg
     1081 ctgctcgccg cgccgttcta ttgcgatacc caactcgtct tctactcgac cggggcgata
     1141 cgcgaggccc ctcgcgccga ctggacgctc gccgacatgg agcgcctggc caaggcctcg
     1201 ggagcccgcg tgggcgcggc ctggaacgcg tattcggcgt actggttcct gcccttcgtc
     1261 atcggttacg gtaaggagtc agtcgtcggc cccgacggcg ggatgaacgt acggcacccg
     1321 gcgtacggcg ccgcgctgac ctatctcagg gattcggccg cgcggggctt cctgaccccg
     1381 atggagcgag acgccatgat ggcgtacttc acctcgggca aggcggcgtt catcctgtcc
     1441 gggtcgtaca gcatccccga gttcaaacgc ctcggcatac cgttcggcgt cgcgccgttc
     1501 ccgctggtct ccgttgatgg caagcgcctg gctccgatgc tcgactataa gggcttcgcg
     1561 gtcacgagga ccacgaagag cccggtgctg gccaggcggc tcgtccagta cctgtccagc
     1621 gccggcgtcc aggccgcgtt ctgcgggccg cagggcaagc tccccgcgga cgaacgcgcg
     1681 tggagcctgc taccggccga cgacccgtac caggcgacca tcagggccag ctgggaagct
     1741 ggcgcagcgg tgccgcccga ccccgtatac ggagatttta agaacgcctc gtggaaactg
     1801 atccgtctct acctgggcgg ctcgacgacg ctcggagaga ccatggaggc gctcgcgcgg
     1861 atcatgggcg aatgaccggt cacgtaggga tgacaccaca tatacagtca aggagaacga
     1921 gcatgaaacg aaccatatgg atgggcggca ccgccctggt cgcggcgctg gcgctcgcgg
     1981 tcttagccgc ttgcgtcagc caagacaagg ccgtggccca agaagaagtc cccgtcgacg
     2041 cgacgagcgc cgcgacggac ggcacgagcg gcgccaccga ggccgcctcc tcgtcgggag
     2101 gcgccggagc caagggtgac cggtccttcc aggacggctc gggctgggcg gtgtccctga
     2161 agggcattcg cgaggccacg ctctggcaga gctactacga ggaggccgcg aagcacgcca
     2221 gccactacgt cgagaagaag gtagacaaga agggcgtcat gacgagctac cggggcatgc
     2281 ccctcaggct ggccatcgcg atgatcgacg gcgcggagaa ggaccacgcg tggctcttcg
     2341 acgccgccct gtgggccgcc ggctacgacg tcacgctcgt cgcctcggat ggctactccg
     2401 tcaccttcaa taccaaggac gtggccgccg acgccctgat catcgccgac cgcgaggacg
     2461 gtacgaagat acctccgatg gtcgtcggcg acgctgccaa gaacctctgg gtccgcgacc
     2521 tcgccatgat cgagacctcg ctggccccca gcgcgctcgt cgcggccgcg gcctcgttct
     2581 ccctggacct ggacatcaac ggcgccaagg ccagctggag cctcgccgag ctacagagac
     2641 tgccgatcta cacggagggc cggggagcct acaccaccag cgccggcacc cgctacgagg
     2701 gcgtctacgg cggcgtcagg ttgctcgacc tgctcaagac ctacgccgac ataggcgccg
     2761 acgactccgt gaccttcgtg gcgatggacg gctacgagat gagctatccg ggcaagaccg
     2821 ttctcgacca ggccgacgga gaatggctcc tggcgttccg gctcgacggc gactggctac
     2881 ccaaggatcc cggctacatc cgcaccatca aggtcggccc gaagaccccg aacatcgacg
     2941 ggcacctatc ggtccgcatg atcaagaaga tagtcgtcaa gcagaaggac tacgtcgatt
     3001 tcaccctcag cctctcgggc aagatgcgat tcgacctcga ccgcggcacc gtccagagct
     3061 gcgtcagctg ccataccagg accgtcacct tcgagcgcaa gggcgtcgta gccgagtaca
     3121 ccggcttccc gctatggctg ctcctgggtt acgtcgacga ccccgagtac gcgccgcaca
     3181 agcaggacac ctccatcaag acctacatgg acgacgccgc cgacgcgggc tacgccatca
     3241 agctgtcagc catcgacgga tattcgatta tcctcgactc cagggacatc acccgcaacg
     3301 acgacatcat cgtcgcgatg taccgcggcg gcgagaagtt gccccccgag gaatcgccgc
     3361 tagtcctcgt ctgggaccgc gcggccaaga aaattcccga gggcatcaag aacgtgaaga
     3421 tgctcgccag cgtcggagcg acgttctgac gaagccctcc gaacggaccc gggaccggcg
     3481 ctcggcctcc gtcccgggcc cgctcgccgt cccgggcctg ttcgccgacc acggccgcct
     3541 cgcgccgctc gtggccctcg tcgtcctggc gggggccgcc ccgttcctct cggctctccg
     3601 ggagagcttt ctctcgggcg gcgcgtacat cggcctggcc aactaccgcg agctgttcgc
     3661 cgaccgcggt ttcccgatgg cggcagccat cacgataggc tggagcctcc tgtccgcctg
     3721 cgccgtcatg gttctggcct accccatcgc gtcgctggcg ctcggctcgc ggcgcgcgta
     3781 cgccataatc ttcccgctcc tcgtggccat gtggtcggtg ccggtctaca tcggcgcgcc
     3841 gctctggcgc ttcgtcctgc acggcgcggc cggcgactcg gtgttccgcg ctctgaccgg
     3901 cattgaagtc aacctgatgg agtcgccggt cgccgccttc gtctccagcg ccctggtggc
     3961 ggcgtggttc aggttgccgc aggccgtctt catcatgctg gccgccctcg gccggtcccg
     4021 ccgctccacc gacgacgccg cccggataga cggagcgggt ccggcggcgc tggccttcac
     4081 cgtacggcta ccggccatgg ccggcgccat cggcgcggta gccgccctgg agatcgtatc
     4141 ggccttcaag gagttcaccg tgccgttcct gatgacggcc ggaggccctc cgctccgcgc
     4201 cggcatcacc gagcgcacgg tcgtcggcgc caccacgacg ctcgagctgt acctctatga
     4261 tctcttctcg ggctatgccg actcgggcgt cgtgtccgcc tacgccgtct gcctgtcgct
     4321 cgtcgtcggc gcggccgtgg ccgggggctt cgccgtcagg gccgccctca agcggcgcag
     4381 cccgctatcg cgcgccgagt cccggaccgt cggcccggct ccgtcgccgg acgcctctcc
     4441 gtcgccgcgc gccgagtccc ggaccgtcgg cccggctccg tcgccggacg cctctccctc
     4501 gccggacgcc tctccctcgc cggacgcctc tccgtcgccg cgcgccgagt cccggaccgt
     4561 cggcgcggcc gtggccgggg gcttcgccgt cagggccgcc ctcaagcggc gcagcccgct
     4621 atcgcgcgcc gagtcccgga ccgtcggccc ggctccgtcg ccggacgcct ctccgtcgcc
     4681 ggacgccgtg gcggacgccg gctccagccc gtccgatctc ggcggctccg cgccgtccct
     4741 gggcggcgcc cgcgccccgc ggcggatcgg ccgagtgccc ggcaggctcg cggacgccgc
     4801 cttcgccggc tcgtcctggc tcgtcgtcgc cgtcctcgtc gcggcggccg cggtgctggc
     4861 cttctgtatc ctgtggatgg ccttctcgga cctgtccatc gcgttcatcg actcgccgat
     4921 accgcgcttc ttcaccacgg ccaacttcgg caaggccatc ttcgacgacg gcctctggct
     4981 ggctttggcc aatacggtct acgtctcggc gctgacggcc gcgctggtcg gcgtcgtcgc
     5041 cttcccggcc gccgcctggc tggcggaccg gccccgcgcc agggccgcgg cgttcttcgt
     5101 cgcgctgcag gccctctcca gcgccggagg cgtccactcg ctaattccgc tgtacgatct
     5161 ctggcgacgc ctgggcctgc tcggcggcta cgccccggtc gtcctcgtgt acctgtacca
     5221 cagcctgccg gtcgcgctct tcgcgctggc ggagttcatg cgcgaccaac cgccgtcgtt
     5281 caaggacgcg gccaggctgg aagggctcgg cacgctcggg tacctctgga gggtacagtt
     5341 gccgctcgcc ctgccggcca tcggggccgc agcgatggtc gccttcctat cggcctggaa
     5401 cggcttcatg gcgccgctcg tatttctgga cgacgacgcc acgtacacga tagcggtcaa
     5461 gctacacgcc tacgtcggct ccatcgcgtc aggctcgccg aaatggaacc tcttcgccgc
     5521 ggcgtcgatc gtgaacgtcg cgatcatcgg attgttgttc aggcgtttca agaaacccct
     5581 cgccacctcc gcgttgtcgg accaaatcga cgactagcgg ttcgccgagt accggacggg
     5641 ttcgacgccg cggaccgcga gcgaggagcg ccacgcgttc gcgagcacgc cgaaggccac
     5701 tccggcgttc aggctgccct tagcgcccag catcggtatg ctgaccgtcg cggtgcagag
     5761 ggccatcgcc tcgggcgata ccccgagctc ctcggagccg acgacgacga tgccccgctc
     5821 ggggaacgcg aagccgtcta cgctctcgcc gcgtagctcc agagccagta ccgcgccgcc
     5881 ggcctcccgt tccgcctcga gggcctcgag ccccgcccgc ctccagccga ccgcctcgac
     5941 cgcgcccatc gccgagcggg cggccctcgg gtgcgccggg tccgcggtga agccggacag
     6001 cagcagctcc gcgaagccga aggcgtcggc gctcctgaag atggagccca cgttgaacgg
     6061 cgagcgcagg tcctcgaggt aggcgcgcag gccgggaaac acccggcgct cggatacggc
     6121 gagcgatgag ccgagaggct cgcgaaggtc ccagtcggcg ggagagagtc cggcgtgggc
     6181 ctcgaggccc cgcgatagct tgtttattaa gcgaacgccc gcctccggcg acgatagggc
     6241 ggacttgccg gcctcggacg aggcctcggc cgcgagcgcg cgcagctcag ccgacagcga
     6301 gccgtccgcg gcgaacgcgg cgccgagttc ggctatccac gcgcccgcga tggttcggcc
     6361 ctgcctgtaa tcggcctcca ggtgccccag gacctgggcc gccttgcgcg ccctatgcaa
     6421 aggcgacagc gtcatcaact tccgtacggc tatcatttaa tatgccggat aacaaaaatc
     6481 ggaaccacgg aggcgcggag acacggagta aaaaggaaag gacatagcca agaaaaggtt
     6541 catgacctta tcccaggctt gttattttct cccgcatcct tctttaccat cgctcttcct
     6601 ctccgtgcct ccgtgtctcc gtggcagatt tcctatactg gattacttta ataaaccgtt
     6661 ttgatgaagt cgaagacgtc gtcgacggtc atggtgcgcg gcaggaaatt catgaagtcg
     6721 aacttgcgcg ccgactcggc ggtctcgacg aggcgctcga ggggtagctc gaagtccttg
     6781 agccggctcg gtatcttgag ctgaccgagc ctcgtgcgta tggactcgac ggcgcgcgcg
     6841 gcggctcccg cggcgccctc gtccggctct atctcgcaga acagcggggc cagggaggcg
     6901 atcttctccg ggcgcgaccg agacagggac tcgagcaggt acggcaggat gaccgccgcc
     6961 aggttggcct tgggcacgcc ccagcgggcg ttgacggcga aggaaatggc cgtgccgagg
     7021 ccgggcgagg aggcggccag gccgaggcct gccaggaagc aggcccgggc tgcgtccatc
     7081 ctggcctggg gatcctcggg acgctggagc gtcgcgtcga gggctcgtat atagaggccg
     7141 gcggcctcct tcagggtcat atcggacagg aagccctcgc gcgcggaggc gtagccctcg
     7201 atggctatca tcgcgccgtc gagcagggcc tcggcggacg acttggcgga caggccgccg
     7261 aaggcgttcg ggtcgagcac gacggcggtc tcgatgccgc gcttggcctg caggaacacg
     7321 gcgcccccgg tcctcgcgtc ggtcagcacg aggccgccgg acagcaggaa ggggtcgcgg
     7381 tagctcgtcg gcacgagcgc gagcggtagc ggggggcgca cgggcggctc gccgtctatc
     7441 caggcgtcga cgccgcgctc gcccgatgat ccggccgggg cgagggcggc gaccgccttg
     7501 gccacatgca tggccctgac gccgccaagc cctatgacca acgggacgcg ggcgcccctg
     7561 gcgagcgagg ccgcctcctc gacggcggcc gacgacgggc gctcggccag gtcgtcgaag
     7621 gtgatcagct ggatgccgcg gccctcgagg atggcggcga cgcgctccgc ggtcttggcc
     7681 tcggccaggc cggggtcggc cacgagcagg gcccgctcgg gcccgccgtc gagcaccagg
     7741 ggcagcttat agagggcgtc ctgcccgata tagcagtcag ccggaacgtg gaacctgaaa
     7801 tccgccatcg ccgattctcc tgcactatcg cccggcccgg cgtcctaagc gtcgccggag
     7861 gccgggcaat aaaaaggacg gaagcgcaag cctccgccct cgtttggaca ccatgaagac
     7921 gctcagagca actggtcgag tatgcgatcc gccgtggcgc tcaggatcgc gtcgttcagg
     7981 tagtcggggt cgtcgatctt ggccttgagc tcagcgacgc gatccatcct gatctccggc
     8041 gccgccttgg ccagctcgag agccttgaag agctcggcct tctcgacggc ctccgaggac
     8101 aggctaacgg aatcgctgcc agaattcctc tcggcgcgag cggcgcccga gggtttcttt
     8161 ggctggatcg gatccagcgg gttcagccta tcaattgtca tcggtggccc ctgcctttca
     8221 catactcaat atcggcaccg taaggcgttt agtttacccc tttttcgccc cggacacaag
     8281 tagcgctatc tcgcccttga cctcgggccg cgccgcgagc aggtcccgga cctcggccgc
     8341 cgatccgacc aggaattcct cgtggagctt ggtcatctcg cggccgacgc acaatcgcct
     8401 ctcggcgtca atatcggcaa tatccgtaag gagcttcact atgcggtgcg gcgattcgta
     8461 catgacgaag gcctcgtcgc gcgacatgag ctcggcgacc ctggaacggc gccggccggg
     8521 cttcggcgag gggaagccgt caaaggcgac agtcttatag aagatgcccg acgccgagac
     8581 cagcgcggcg aaggcgctcg ggccggggat aggcacgacg tcgtggccgg cctcgcgggc
     8641 gcgggctgcc gccaggatgc ctgggtcgga gaggcagggc gtgc
//
LOCUS       DAOPHV010000038         8528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_10472_length_8528_cov_4.178921, whole genome shotgun sequence.
ACCESSION   DAOPHV010000038 DAOPHV010000000
VERSION     DAOPHV010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10472_length_8528_cov_4.178921"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLQ29_01865"
     CDS             <1..489
                     /locus_tag="PLQ29_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85419.1"
                     /translation="LICVRGRTGFGARRRRRGRLDPVRRLLRFLGNQELGEGHPDEDH
                     QREREQRASVKSSGSSLLHSCTPRLAGRAAGVGRSISRDFMVLDHAAPGSPPGSSGMA
                     TLIGGKATVLRAMAEKAADHVCMALGVDEVCRTRDYVLPPWREYYSEGNYAEGATLGG
                     RR"
     gene            486..1268
                     /locus_tag="PLQ29_01870"
     CDS             486..1268
                     /locus_tag="PLQ29_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPG85420.1"
                     /translation="MSDTVSASAAIPLRLRIRDGDGYAEFRVEAEPHRTVLDLLERLR
                     SGGSAVPAYRHSCHHGSCGTCGAVIDGVEALMCLTRAADLAAPRPRLPGGPAVPPERD
                     EAGAVIVTLEPLSKMTVVSGIAVVPGPVFAGIPKGASYLRPASADGRASLPPDPAEAV
                     ADPGAWRPDGRVRFEACIECGLCVSACPVAVPFIGPAALAAVNREREKRPEQVSAMVA
                     IAAAPDGVGPCGRHLACSRVCPQAVFPGKHIQLLRNALASLA"
     gene            1369..3290
                     /locus_tag="PLQ29_01875"
                     /pseudo
     CDS             1369..3290
                     /locus_tag="PLQ29_01875"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
     gene            3287..4420
                     /locus_tag="PLQ29_01880"
     CDS             3287..4420
                     /locus_tag="PLQ29_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85421.1"
                     /translation="MIRSALSKITCAVDSRLSAIIDEALSDMAIPEAFIQRAKQVSFI
                     ERSGPFGLAARAALSESRAELYRFYVPAGLESAAARRVAEAADLYLPGRGTLFAEDVE
                     VLSAEAPAYDESAISSACEEGSCSRPLEHYSVLCCIVQRGMGEDLARTVLEMGLCVPI
                     VSFGEGMGLRNKLGLLRITIPVDKEILYFVVPERDTALVEGVAVHKARLDKPGQGFIY
                     RYQVRAYAVNLRVRAGKRSHAASMEQVISVLDELRGSSDWRRLEPVKGRATKGKAGSA
                     ASNLACLSILAEEGSVGGFVRAAMDAGAGGATLVPLTRRRYDGFVEAGADLSSAREGC
                     DLIIRTDLVEAVLGAVGRLGLFEGDARGQAEISAVGHAVTYSA"
     gene            complement(4437..6431)
                     /locus_tag="PLQ29_01885"
     CDS             complement(4437..6431)
                     /locus_tag="PLQ29_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPG85422.1"
                     /translation="MRGMMGKVLIVDLTTGTMEEKAIPEETYRDYLGGVGLAVRLLTD
                     MIPVGADPLGPGNALAFMSGLLTGSGSMMTGRWMVCCKSPLTGGWGDANCGGTFSPEI
                     KRCGWDGIIFRGVSPKPVAFVADENGPRLVDASAWWGMDAIESEDAIKASWSGKKKPA
                     IATIGQAAEKLSLISGISNDGGRYAARSGVGAVMGSKRLKALALAGSKPIVCADPARV
                     RDLSKAFAAKIGKLKLPKVFTGAFMPLLGKMLGLKVVVPVDGILASAILKKWGTIYNN
                     TAGLVNGDSPVRNWGGSVKDYGASRYRSVNPDRLRERESKKYNCFSCPIGCGGICDAT
                     GTGAAGKHMHKPEYETCCAFGALVLNDDLDTIMLCNDICNRSGLDTISAGTVVAFALE
                     CYERGVITKEDADGLELRWGDSGAIVELLRMMAERRGLGDLLADGVAIAAKRLGKGAE
                     ELAVHAGGQEPGMHDGRMDPMMAVSFAADPTPGRHTISAGVYYNVSHLWEYVSWAPKV
                     TKPYPKSKEYAASDEETLKSVAGACMKQLLDATGGCLFALTTGLQHWRFFDWLAAATG
                     DARTPDEWMEAGRRIQTARAAFGARERLADEGKAWSRNYSLANPRIYGDPPLREGPLA
                     GKTVPLAEMVGRYRREFGWDEDSGAPRPETMRTLGLSVHS"
     gene            complement(6451..6684)
                     /locus_tag="PLQ29_01890"
     CDS             complement(6451..6684)
                     /locus_tag="PLQ29_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HPG85423.1"
                     /translation="MKVRVYVPPFADPRAIDDDGFVELADGATLGALLKRLRIPLRPL
                     AATFCLVNYERAGVGHRLADGDTVSFFSLLPGG"
     gene            6840..6921
                     /locus_tag="PLQ29_01895"
     tRNA            6840..6921
                     /locus_tag="PLQ29_01895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6874..6876,aa:Leu,seq:cag)
     gene            7258..>8528
                     /locus_tag="PLQ29_01900"
     CDS             7258..>8528
                     /locus_tag="PLQ29_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPG85424.1"
                     /translation="MVSTGSRRILLVEDQAVIALNERRLLESLGYEVAMAYSGEQAIE
                     AFKADGSIAMAVMDIDLGDGIDGTEAAGEMLKAREVPVVFMSGHAEADIVEKTERVSS
                     YGYIEKNSGPKFVAATLRMAFRLFEARSSMQEANRRLEATLEALPDLLFELDEDGRYL
                     AVHCNDPGSLYAPIDGVIGTSVRDRLPPEAAETVMAALAEAKAGGLSRGRRYDLAFPE
                     GRRSFELSVSRMATGRSRRKFVMICRDITKQAKVEDELRRSKRQAERLLGISAQIIQV
                     VDLDGVVTVLNDSGHRLLGYEPPELIGQRWVDRCVPDESRPGVEAYLSSLFRSGADGI
                     QPHENDVVTKDGRRLRIHWQNSLLRADDGRVVGVLSSGEDVTEYRELEERYRTIVEAS
                     PDNITVTDLQGRMLMFSPKARTMFGVEDGEDR"
BASE COUNT         1280 a         2832 c         2930 g         1486 t
ORIGIN      
        1 ttgatctgcg tccggggacg caccggtttc ggcgcccgac ggcgtcgtcg cggtcgtctc
       61 gatcccgtcc ggaggctgct gcggtttctt gggaatcagg aattgggtga aggccaccca
      121 gacgaggatc atcaacgcga acgcgagcag cgtgcgagcg tcaaatcctc cggatcctct
      181 ttgctccatt catgtactcc acggctcgcc ggacgggcgg ccggcgttgg ccggagtatc
      241 agccgcgact tcatggtgct ggatcacgcc gcgcccggtt cgccgcccgg ttcctcgggc
      301 atggccacgc tgatcggcgg caaggcgacg gttttgcgcg cgatggccga gaaggccgcc
      361 gaccacgtct gcatggcgct cggcgtggac gaggtctgcc gcacccgcga ctacgtcctg
      421 ccgccatggc gcgagtatta ctccgaaggt aactatgccg aaggagctac gctgggaggc
      481 cgaagatgag cgataccgta tccgcctcgg ccgcgatacc gcttcgcctc cgcattcggg
      541 acggggatgg atacgccgag ttcagggtcg aggccgaacc gcaccgcacg gtgctcgacc
      601 tgctcgaacg gctacgatcc ggcgggagcg ccgtgccggc ctatcgtcac tcctgccacc
      661 atggctcctg cggcacctgc ggcgcggtga tcgacggcgt ggaggcgctg atgtgcctga
      721 cgagggccgc cgacctggcc gcgcctcggc ccaggctgcc gggcgggccg gccgttccgc
      781 ccgagcgcga cgaggccgga gcggtcatcg tcacgctcga gccgctgtcg aagatgaccg
      841 tcgtctccgg catagccgtc gtgccggggc cggtgttcgc gggcatcccg aagggggcgt
      901 catacctgcg gcccgcgtcg gccgatggtc gggcgtcctt gccgccggac ccggccgagg
      961 ccgtcgccga tcccggcgcc tggaggccgg atgggcgcgt acgcttcgag gcctgtatcg
     1021 agtgcgggct atgcgtgtcg gcctgtccgg tcgccgtacc cttcatcggg ccggcagcgc
     1081 tggccgccgt caaccgcgag cgcgagaagc gtccggaaca ggtcagcgcg atggtagcca
     1141 tagccgccgc gcccgacggg gtcggccctt gcggcaggca cctcgcctgc tctcgggtct
     1201 gcccccaggc cgtgttcccc ggcaagcata tccagctact caggaacgct ctggcgtcgc
     1261 ttgcatgagc ggcctttttc taggatagtg actctatagc ctatatgagg agcctcccga
     1321 ttgccatcga gcaccgtcgt caagataccg tttaagcgta aactggccat gataggcgcc
     1381 tggggacgat ccaggctcgg agcccaggcc agggccgtcg cgttcgtcgt ggtctacctg
     1441 gtgctggcgc agaccttgct gctgaaagag ccgatacacg atccgctctc ggtcgtcgtc
     1501 ggcatagccg cgacgatagc gggcctggcc ttcttcctcg aggggctctt cctcggggtg
     1561 atgccgctcg gcgagcgatg cggcatcagg ctgcccggcc gggtagggcc gctggtcatc
     1621 gccggcttct ccctggtgct cggcgtgacc gcgacgctgg ccgaacccgc tataggaatc
     1681 ctgaagctcc agggaggctc ggccaggcca tggtcgacgc cgctcctgta tctgctgctc
     1741 aaccgcggct ccgtttggct cgtggcggcg gtggccgtcg gcgtcggcct ggcggtgacc
     1801 ctcggcgtct tccgcttcct gtatcgctgg cccctcaagc cattcatcta cgcgctgata
     1861 ccgctactcg tcgcggtgac cttcttcttc gacgccgacc cgaacctgag ggccgtggcc
     1921 ggtctggcct gggacacggg cggcgtgacc acggggccgg tgaccgtgcc gctggtcatt
     1981 gccctcggca tcggcgtctc gcgcatcgtc ggttcgcgta gcgacgcttc tggcggcctg
     2041 ggcgtggtga cgctcgcctc ggcgttaccg gtggcggcgg tattcgccct ggcggctgcc
     2101 ctggtgccga gcgtgcccgc tcccggaagc ccggccgcgt tcttttcgaa ggctgagcga
     2161 gagcgggccg tgttcgtcgc cggctccgag gaggcgctcg cggaactggc ctccggggcc
     2221 gtggcgtcga gtagcctgac cgcctccgat ttcgtggcgc tgttccccga ctactaccag
     2281 ctcgccggga cctccgccgg cgacgcctct gacggaggga cagcgagcgg gggcctccgc
     2341 gcccgtctcg gcgatagcgc cctgtcggcc ctcaaggccg tgctgccact ggcgttcgtg
     2401 ctgatagtca cgctcgtgtt cctgctcaag gaccggttgg tcggtatcga cgagctcgtg
     2461 ctcggcctgg ttttcgcggt gcttggcatg ttcctgttca acttcggcat ggagaagggc
     2521 ctgaccgcgc tcggtaccca ggccgggtcc agcctgccgc gcgcctacga gcggaccgag
     2581 cggcccgacc gggcgctgct cctgagcggc gttaccgagg cggatgtcgt caaggctgta
     2641 gggcctgacg gtcccgctga atacatctgg atggacggaa cgaacgggcc ggtcgccgtg
     2701 cccttcgacc gggccctatg ggacgaggcg agcggcacgt accgctacgt gccggtcgag
     2761 cgcgccgtct tcgatagatg gggcgagaac gccggcttcg cggcggccct cctgttcgtg
     2821 ttcatcctcg gcttcggcgc gacgctcgcc gagccgtcgc tatcggccct cggcacgacg
     2881 gtcgaggacc tgacgaccgg tacgtataag aaatcgacgc tcgtcggcat ggtcgcgatc
     2941 ggcgtcggcc tcggcctggc cttcggcttc gcgtcgatcc tgttcgccct gcccctacgt
     3001 gggtgcttgg cgtgccctac gccctggccc tcgtcctgac cgccttcgcc ccggaagact
     3061 tcgccgctat agcctgggac tccgccggcg tgacgacggg tccgataacg gtaccgctgg
     3121 tcatcgccac cggcctgggt attggcggcc acgccggcgt cgcctcgagc ttcggcgtcg
     3181 tggcggccgc gagcgtgttc cccgtggtgg ccgtgctcgg ctccgggctg tggcggaccg
     3241 tgcaggcgcg ctccgcgctg gcggcgatgt cccagggggg ccggtcatga tacgatccgc
     3301 gttgtccaag atcacctgcg ccgtcgattc gcggctatcg gccatcatcg acgaggcgct
     3361 ctccgatatg gccataccgg aggccttcat ccaacgggcc aagcaggtat cgttcatcga
     3421 gcgctccggc cccttcgggc tggcggccag ggcggcgctg tcggagtcgc gcgccgagct
     3481 gtaccgtttc tacgtacccg cgggcctgga gtcggcggct gctcggcgtg tcgccgaggc
     3541 ggccgacctg tacctgcccg gccgcggtac gctcttcgcc gaggacgtcg aggtactgtc
     3601 ggccgaggcg ccggcttacg acgagtcggc catctcgtcg gcctgcgagg agggctcctg
     3661 ctcgaggccg ctcgagcact attcggtgct atgctgcatc gtacagcgag gcatgggaga
     3721 ggatttggcg cgcaccgtgc tcgagatggg gctgtgcgtg cccatcgtct ccttcggcga
     3781 gggcatgggc ctgcgtaaca agctcggcct cctgcgcatc acgataccgg tggacaagga
     3841 gattctctat ttcgtcgtgc ccgagcgcga cacggccctg gtcgaggggg tcgcggtcca
     3901 caaggccagg ttggacaagc ctggccaggg ctttatctac cgctaccagg tcagggccta
     3961 cgcggtcaac ctacgggtca gggccggcaa gcgcagccac gcggcgtcga tggagcaggt
     4021 gatcagcgtg ctggacgagt tgcgaggctc gagcgactgg aggaggctgg agccggtcaa
     4081 gggtagagcg acgaaaggca aagccgggag cgccgcgtcg aacctcgcct gcctgtccat
     4141 tctcgcggaa gagggcagcg tgggcggttt cgtgcgcgcc gcgatggacg cgggcgcggg
     4201 cggcgcgacc ctcgtgccgc taaccaggcg ccgctacgac ggcttcgtcg aagcgggcgc
     4261 cgacctgtcg agcgcgcgcg agggctgcga cctgatcatc aggaccgacc tggtcgaggc
     4321 cgtgctcggc gcggtcggca ggctcggcct gttcgagggc gacgcccgcg ggcaggccga
     4381 gatcagcgcg gtcggccacg ccgttaccta tagcgcgtag ccgaccatct cgcgccctag
     4441 ctgtgtaccg ataggccgag cgtcctcatc gtctccggcc tcggggcgcc ggagtcctcg
     4501 tcccagccga actccctgcg gtacctgccg accatctcgg cgagcggcac cgtcttgccc
     4561 gcgagcgggc cctcgcggag cggcgggtcg ccatagatac gaggattggc gagcgagtag
     4621 ttcctggacc aggccttgcc ctcgtcggcg agccgctcgc gggcgccgaa cgccgctcgg
     4681 gccgtctgga tgcggcggcc ggcctccatc cactcgtccg gcgtacgggc gtcgccggtc
     4741 gccgcggcca gccagtcgaa gaagcgccag tgctggaggc cggtcgtcag cgcgaagagg
     4801 caaccgccgg tcgcgtcgag gagctgcttc atgcaggcgc cggcgaccga cttgagcgtc
     4861 tcctcgtcgc tcgccgcgta ttccttggac ttcggatagg gcttcgtcac cttcggcgcc
     4921 cagctgacgt actcccagag gtgcgacacg ttgtagtaca cgccggccga gatggtgtgc
     4981 cgccccggcg tcgggtcggc ggcgaacgat accgccatca tcgggtccat gcggccgtcg
     5041 tgcatgcccg gctcctggcc gcccgcgtgg acggccagtt cctcggcgcc cttgcccagg
     5101 cgcttcgcgg ctatcgcgac gccatcggcc aggaggtcgc cgagaccccg gcgctcggcc
     5161 atcatccgta gcagctcgac gatggcgccc gagtcgcccc agcgaagctc gagcccgtcg
     5221 gcgtcttcct tggtgatcac gccgcgctcg tagcactcga gggcgaaggc cacgacggta
     5281 cccgccgaga tcgtgtccag gccggagcgg ttgcagatgt cgttgcatag catgatggta
     5341 tcgaggtcgt cgttcaggac gagggcgccg aacgcgcagc aggtctcgta ctcgggcttg
     5401 tgcatgtgtt ttccggcggc cccggtgccc gtggcgtcgc agatgccgcc gcagcctatc
     5461 gggcaggaga agcagttgta cttcttgctc tcgcgctcgc gaaggcggtc cgggttgacc
     5521 gagcggtagc gcgacgcgcc gtagtccttg accgagcctc cccagttccg caccggggag
     5581 tccccgttga cgaggcccgc ggtgttgttg tagatcgtgc cccacttctt gagtatcgcc
     5641 gaggcgagga tgccgtcgac cggtacgacc accttcaggc cgagcatctt gccgagtagc
     5701 ggcatgaagg cgccggtgaa gaccttgggt agctttagct tgcctatctt ggccgcgaag
     5761 gccttggata agtccctgac gcgcgctgga tccgcgcaga cgatcggttt ggagccggct
     5821 agggcgagcg ccttgagccg cttggagccc atcaccgcgc cgacgcccga gcgggccgcg
     5881 tagcggccgc cgtcgttcga gatgcccgag atcagcgaca acttctcggc ggcctggcct
     5941 atcgtcgcta tggccggctt cttcttgccg gaccaggagg ccttgatcgc gtcctccgac
     6001 tcgatcgcgt ccatgcccca ccatgccgac gcgtcgacga gtcgcgggcc gttctcgtcg
     6061 gcgacgaagg ccaccggctt gggcgagacg cccctgaaga tgatcccgtc ccagccgcag
     6121 cgcttgatct ccggcgagaa cgtgccgcca cagttggcgt cgccccagcc gccggtcagc
     6181 ggcgatttgc agcagaccat ccatcggccg gtcatcatcg agcctgagcc ggtcaggagg
     6241 ccgctcatga aggcgagcgc gttgcccggc ccgagcgggt cggccccgac cggtatcatg
     6301 tcggtcagca gcctgaccgc gagcccgacg ccgcccaggt agtcccgata ggtctcctcg
     6361 gggatagcct tctcctccat ggtgcccgtc gtcaggtcga cgataaggac cttgcccatc
     6421 atgccgcgca tagaacctcc gatggcagta tcaaccgccg ggtagtagcg agaagaacga
     6481 aacggtgtcg ccgtccgcca gcctatgccc gacgccggcc cgctcgtagt tgaccaggca
     6541 gaaggtcgcg gccagcggcc tgagcgggat gcgcaggcgc ttgagcaagg cgccgagcgt
     6601 cgccccgtca gccagctcga cgaaaccgtc gtcgtcgatg gcgcgcgggt ctgcgaacgg
     6661 cggaacgtag acgcgtacct tcaaggaccc tccgtacggc gtatccggga tcgaaccacg
     6721 ccagctggat catgctataa gccaagccgg tcgatgtcaa acgaaaaatc gagcgaccgc
     6781 tcggcgcgcc gcccgccctt gactctccgc cgccctccag ggtatatact ccctaccacg
     6841 cccagatggt ggaattggca gacacgctag tttcaggtac tagtgccgag aggcatgggg
     6901 gttcaagtcc ccctctgggc aaaaaagcga gatcgcgcta tgcgcggtct cgcttttttt
     6961 ataagcccag agggggactt gaacccgaag cgccctcgcc gcgagagcgg taaaaagcat
     7021 cgcaggatgc gcgtggcagt gtgcgaaggc ggccccgcga cccgtgccta gtattacgaa
     7081 ccttattgat cctgtaatcg catatccggg tatcgtgcaa gatagctcga atcgcatcga
     7141 cgcatcgccc tagcgcagtc tcgagtatcg gctagcgaag catcgtgatc gcatacataa
     7201 ggttcgtaac accgaagaag acttgcatta gctcgtgcct cgtggaacac tatacgcatg
     7261 gtctcaaccg gctcgaggcg tattcttctc gtagaggatc aggccgttat cgcgctgaac
     7321 gagcgccgcc tgctcgagtc gctcggctac gaggttgcga tggcgtattc gggcgaacag
     7381 gccatcgagg cgttcaaggc cgacggatcg atagccatgg ctgtcatgga tatcgacctg
     7441 ggcgatggta tcgacggtac cgaggccgcg ggcgagatgc tcaaggcgcg cgaggttccg
     7501 gtggtcttca tgtctggcca cgcggaggcg gatatagtcg agaagaccga gcgcgtctcg
     7561 tcgtacggct atatagagaa gaactcgggg cctaagttcg tggccgctac cctgcggatg
     7621 gctttcaggc tgttcgaggc caggtcgtcg atgcaggagg ccaatcggcg gctcgaggcg
     7681 acgctcgagg ccctgcctga cctgctgttc gagctcgacg aggacggccg gtacctggcg
     7741 gtgcattgca acgaccccgg aagcctatac gcgccgatcg acggcgtcat cggtacgtcg
     7801 gtccgcgaca ggctaccgcc cgaggcggcc gagacggtca tggctgccct ggccgaggcc
     7861 aaggccggcg ggctgtcgcg cggccggcgc tacgacctgg ccttcccgga gggccggcgc
     7921 tccttcgagc tgtcggtgtc gcgcatggca acaggtcggt cgcggcgcaa gttcgtcatg
     7981 atctgcaggg atatcacgaa gcaggcgaag gtcgaggacg agctacgacg cagtaaacgg
     8041 caggcggagc gcctcctcgg catatcggcg cagatcatcc aggtcgtcga cctggacggt
     8101 gtcgtgaccg tgctgaacga cagcgggcat cggctgctcg gctacgaacc gcctgagctc
     8161 atcggccagc gctgggtaga ccgttgcgta ccggacgagt ctcggcccgg ggtcgaggcg
     8221 tatctgtcat cgctcttccg tagcggcgcg gacggcatcc agccgcacga gaacgacgtg
     8281 gtcaccaagg acggacggcg cctgcgcatc cattggcaga acagcctgct ccgcgccgac
     8341 gacggccgag tcgtcggcgt gttgagctcg ggggaggacg tcaccgagta ccgcgagctc
     8401 gaggagcgtt accggaccat cgtcgaggcg tctcccgaca atatcacggt gaccgacctc
     8461 caggggcgca tgctcatgtt ctcgcccaag gccaggacga tgttcggcgt cgaggacggc
     8521 gaggatcg
//
LOCUS       DAOPHV010000039         8372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_10743_length_8372_cov_4.432728, whole genome shotgun sequence.
ACCESSION   DAOPHV010000039 DAOPHV010000000
VERSION     DAOPHV010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8372
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10743_length_8372_cov_4.432728"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLQ29_01905"
     CDS             <1..403
                     /locus_tag="PLQ29_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85425.1"
                     /translation="HQGGRDPYAISSEERMRRYKDKYAGGQADRGQEPGRPADGQQRQ
                     SQQRQSQQRQGQSRRQGQQPAQGQAQRQQPRQAQRPASQGTKPQASKPQGTGPRAQGS
                     MPQANNAGRPAKPGLIDRIKRAFGLGKRADD"
     gene            419..1537
                     /locus_tag="PLQ29_01910"
     CDS             419..1537
                     /locus_tag="PLQ29_01910"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPG85426.1"
                     /translation="MNDRGDFRRPRLDPEQLFSPFDRALGTLHERLFGRYRLVTVAAS
                     ILIYSAAVLSTGASLGVSTNYFVLLPVICASMGYGLFAGVVAGIVALPGNLLLYRALG
                     HPEFAPASKAMAELSGILVGTVLGYLSDYHRKLEVERYLRKETESELRRALRDREALF
                     REVHHRVKNNLNLIKSIIGLQARRSADPAFKDAAAMLTGRIMSISFVHERLYRTSELS
                     AVAVDEYLKDLVNAVVMAAGGSDTCPTVAFDLSRHDASMDVAVPLGLIVNELVTNSIK
                     HGQPRRGRPLAMEIRLSTDGDELSLSVRDDGVGLPGVDEGRRLAIEEAVLRFPGSLGL
                     TLLDLMASQLGGKAAFSRSDGWTEFNLIFRDAAAAERP"
     gene            complement(1547..1864)
                     /locus_tag="PLQ29_01915"
     CDS             complement(1547..1864)
                     /locus_tag="PLQ29_01915"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPG85427.1"
                     /translation="MQLSLVRPHEPFIIRSVSVGREVGRRLADMGCTEGTEGVIVRRG
                     GFGGPMQVRIHGYDLLIRRDEAARIDVETVGPVRGPGRGMGRGRGRGGRWWAKSGEAE
                     DLS"
     gene            1989..2975
                     /locus_tag="PLQ29_01920"
     CDS             1989..2975
                     /locus_tag="PLQ29_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPG85428.1"
                     /translation="MRRFLKVVIVVSGLALLVTLAASIALASRMRDGARSPDARQGGQ
                     ALFHILAVVPASDAERYYARAVEAMTRTARKRGAVLQTLEYDQDNAPSAIARQLRLAA
                     ELEPDGVVVSAPLDPLVTEAVDTLSKRGVPVVALETDLPGSARAAFIGTNPYSVGVLA
                     AEAVAEDFPGGAEVALVLSRDYADGSARGATIAAGFVYGARDANGVRLAMTRTAREGP
                     SASEAIVRELLAEHASIDVAVFIGARDSEGAARALIEYDRVGELAIIGFDDDPALLEL
                     VHLGVVAASIARSPERSGEVAVEAIMTLAKGGRVSAYIDPGLTVIRRREGER"
     gene            2972..4453
                     /locus_tag="PLQ29_01925"
     CDS             2972..4453
                     /locus_tag="PLQ29_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPG85429.1"
                     /translation="MRRRLLASFRSRMITASLAPIILMGAAATFNLAVSSANLARVAN
                     LFSLDLFIEEALISVDAVVTNLAVYIDTKNSDALRACMHHSKLLSAKAGVLAEGLSYD
                     EDAIIERNLAVLLRRLIEESEAAIRAKRGRNIPEYEARYQDVRMLASFIRDRADALSL
                     ERLGLNLDAFESLAKDGGRAATLLGAMLASAIAFAVAIVLSYSLKLTEPLDALAAAAG
                     RISTGDFSGGPLAASADDEIGQTARAFNVMQASIAAYIDEIRNKAAVESSLMEERLKN
                     LEMQGHLRSAELKALQSRINPHFLFNTLNAGIQLATVEDADRTRVFLERLAGLMRYSF
                     RDLDAPVALRDELACLRDYAYLMTIRFPESIDFDISAEPGAEAAALPKMIIQPLVENS
                     IRHGFADKPLGGAVRVRASLDGGDLVVEVEDNGSGIPDSRAAEIVAAADAGTELGSAE
                     GGLGMANVIARLRLFAGRREVIEFANAVPGARVVVRVPFKEAF"
     gene            4453..5904
                     /locus_tag="PLQ29_01930"
     CDS             4453..5904
                     /locus_tag="PLQ29_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPG85430.1"
                     /translation="MTAKIFIADDERPVIDGVAAAIRKRLPDADICGTAGTGRDAVDG
                     AVRERPDVILMDVRMPGMNGLEALRELRSSAPETVPILLTAYERFDVAKEAFGLGVYD
                     YLVKPVDQDRLVEAIRGALDAAAARKAEAARARNAIEALESRRPLVEEGLVYAALSGD
                     SGGARILDYLSALGFGPEGGDFVAVRRSDSGRRPSDDDERALRASLGYHAACAVGAPA
                     CGVIPVFYPESESASIEGALLTALQEPELRGMTANIGTCRPRDRLAEAWREALAGLAR
                     ATDGSVSHSRSIGVATPRSREPLQAALEAATVGDAAAASAAWAALPDDRLLRAAAAGA
                     IAWSLRHDPEAVVEAAAAVSEGAAPRAAFNSGKPGSGYGATGRVADALRCIEERYAEQ
                     ISLEDVATQVGLSATHLSRVLSNETGKSFIEHLSDYRIARAKAELASGRMSVKEVGAA
                     VGYLDPNYFSRAFKRVTGLTPSDYAKSAGRGDI"
     gene            5901..7004
                     /locus_tag="PLQ29_01935"
     CDS             5901..7004
                     /locus_tag="PLQ29_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPG85431.1"
                     /translation="MIGGGTKGAAARRRRAIMAAAALGVAVLGVAAIALGLALRQGAA
                     RRSREPKPDGAGISIGFSMDSFVEERRARDRDVFVAMARDLGATVNVQIGDEDPSIQE
                     EQILFLLDSGVDVLVVIPSDPAGLGRAVAEAKRRGVPILAYDRLILNGNVDLFLAFDA
                     ERSGELQAEAFLASQGGGRVALYNGRRSDPNAAAMRAGVMNTLRPDIDEGRVIVVADY
                     ESAERRSEEAYQIVDSMLEAGTTFDAVLAVDDMAAEAIIRALSVRRLAGSVFVAGADA
                     DLAACQRIAEGTQSMTVYKPIDQIAAKAAELAVYLARGDRVTVHNAIWDGSYRIPFYE
                     LEPVSVTAAELMATVVKDGFHLEEDVYRNVLDR"
     gene            7181..8191
                     /gene="mglB"
                     /locus_tag="PLQ29_01940"
     CDS             7181..8191
                     /gene="mglB"
                     /locus_tag="PLQ29_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HPG85432.1"
                     /translation="MKKSFAILATLVVVGALLASCGGSADAGGTPKIGVAIYKFDDTF
                     MSYVRNAIEANATGKATIEMVDSQNAQPTQNDQVDAFLSKKAKVIAINPVDRTAAAAI
                     IDKAKGRSTPVVFFNREPLPEDMKKWDKVYYVGAKAEQSGSMQGEIVVDYFKANPKAD
                     KNGDGVIQYIMLKGEPGHQDAELRTEFSIKAVTAAGLKVEKLAEDTAYWDRPKAVEKM
                     KAFYAKFGDRIEVVLCNNDDMALGVIEALRQEGFFSGDKYLPVVGVDATAPALQALEQ
                     GTLLGTVLNDAKNQGKATFDIAYALATGKDPAQAGWTISDGKYVWVPYQKVTKDNYTQ
                     FK"
BASE COUNT         1481 a         2847 c         2734 g         1310 t
ORIGIN      
        1 ccatcaggga ggccgcgacc cctacgccat aagttccgag gagcgcatga ggcgctacaa
       61 ggacaagtac gcaggcggcc aggccgaccg cgggcaggaa ccggggcgtc ccgccgatgg
      121 acagcagagg caaagccaac aacggcaaag ccaacaacgg cagggacagt cgaggcgcca
      181 aggccagcaa cccgctcagg ggcaggcgca gcgccaacag ccgcgccagg ctcaacgccc
      241 ggcatcgcag ggcacgaaac cccaggcctc caaaccgcaa ggaaccgggc ctcgggctca
      301 aggcagcatg ccgcaggcta acaacgccgg gcgtccggcc aagcccggcc tgatcgaccg
      361 gatcaagcgg gcgttcggcc tcggcaagcg cgccgacgac tgagcggccg agggcgcgat
      421 gaacgatcgg ggcgacttca ggcggccgcg cctggatccg gaacagctgt tctccccgtt
      481 cgatcgcgcc ctcgggacgc tccacgagcg gctgttcggc cgctatcggc tggtcaccgt
      541 ggcggcgtcc atactgatct actcagccgc ggtgctatcg accggggcgt cgcttggcgt
      601 ttcaaccaac tacttcgtac tgctccccgt tatctgcgcc tcgatgggct atggtctgtt
      661 cgccggcgtg gtcgcgggta tcgtcgcctt gcccggcaac cttttactct atcgcgcgct
      721 cggtcatccc gagttcgcgc cggcctcgaa ggcgatggcc gagctttccg gcatactggt
      781 cggcaccgtg ctcggctacc tttcggacta ccatcgcaag ctcgaagtcg agcgctactt
      841 gcgcaaggaa accgagagtg agctcaggcg ggccctccgc gaccgcgagg cgctgttccg
      901 cgaggtacac caccgggtca agaacaacct gaacctgatt aagagcatca taggcctcca
      961 ggccaggcgt tccgccgacc cggccttcaa ggacgccgcc gccatgctga ccggcaggat
     1021 catgtcgatc tcattcgtcc acgagcggct ataccgcacc tccgagctgt cggcggtagc
     1081 ggtcgacgag tacctcaagg atctggtcaa cgccgtggtg atggccgccg gcggctccga
     1141 tacgtgcccg accgtcgcgt tcgacctgtc gaggcacgac gccagcatgg acgtcgcggt
     1201 accgctcggc ctgatcgtca acgagctggt cacgaattcg atcaagcacg gccagccgag
     1261 acggggccga ccgctagcga tggagatacg gctaagcacg gacggcgacg agctatcgtt
     1321 atcggtacgt gacgacggag tgggcttgcc cggcgtcgac gagggtcggc ggctcgccat
     1381 cgaggaggcc gtactgcgct tcccgggtag cctcggcctg accttgctcg acctgatggc
     1441 gtcccagctc ggcggaaagg ccgcctttag ccgttccgac ggctggaccg aattcaacct
     1501 tatcttccgc gatgccgcgg cggctgaacg tccctagcca actggctcag gatagatcct
     1561 cggcctcgcc ggatttcgcc caccatcgcc cgcctcggcc tctgcctcgg cccatgcccc
     1621 gtccgggacc gcgaacggga ccgacggtct cgacgtcgat cctggccgcc tcgtcccggc
     1681 ggatcagcaa gtcgtatccg tggatacgca cctgcatcgg cccgccgaag ccgcctcgcc
     1741 tgacgatgac gccttccgta ccctcggtac agcccatatc ggcgagcctt cggccgacct
     1801 cgcggccgac gctgacggat cggatgatga acggttcatg aggccttaca agagaaagct
     1861 gcatcgaatg cttccttaaa gaaagaacgg cggtagaggc cgatattgat tgtcattatc
     1921 atcatcggaa ggcgaatggt caagcgcgct tctttgacgg aagtcgataa cgaggatatg
     1981 cttgctcggt gcgcaggttc ctcaaggtcg tcatagtcgt atccggcctg gccttattgg
     2041 tcacgttggc cgcctccata gccctcgcct cgcggatgcg cgacggcgcg cggtcgccgg
     2101 acgccaggca gggcgggcag gccctcttcc atatactcgc cgtggtgccg gcttcggacg
     2161 ccgagcgcta ctacgcccgc gccgtcgagg ccatgaccag gacggctagg aagcgcggag
     2221 ccgtgctgca gacgctcgag tacgaccagg ataacgcgcc gagcgcgata gccaggcaac
     2281 tacgcctggc tgccgagctc gaaccggatg gcgtggtcgt ctccgcgccg ctcgacccgc
     2341 tggtgaccga ggccgtcgac accctctcca agcggggcgt accggtggtg gccctcgaga
     2401 ccgacctgcc aggctcggcg cgcgcggcct tcatcggcac gaacccctat tcggtcggcg
     2461 tgctcgcggc cgaggccgtg gccgaggact ttcccggtgg cgccgaggtg gcgctcgtac
     2521 tgtccaggga ctacgccgat ggcagcgccc gtggcgcgac gatagcggcc ggcttcgtgt
     2581 acggcgccag ggacgcgaac ggcgtccgtc tggcgatgac caggacggcc cgggaaggcc
     2641 cgtcggcgag cgaggcgata gtcagggagc tgctggcgga gcacgcctcg atcgacgtgg
     2701 ccgtgttcat cggggccagg gactcggaag gcgccgctcg ggccctgatc gagtacgacc
     2761 gggtcggcga gctggccatc atcggcttcg acgacgaccc ggccctgctc gagctcgtcc
     2821 acctcggagt cgtcgccgcg tccatcgccc gctcgcccga gcgctccggc gaggtcgcgg
     2881 tcgaggcgat catgacgctc gccaagggag gccgggtgag cgcctacatc gatccgggcc
     2941 tgaccgtcat acgccgacgg gagggggaac gatgaggcgg cggctcctgg cgtcgttccg
     3001 ctcgcgcatg atcaccgcta gcctggcccc gatcatcctg atgggcgcgg cggcgacctt
     3061 caacctggcg gtgtcgtcgg ccaacctcgc gagggtggcc aacctcttct cactcgacct
     3121 gttcatcgag gaagcgctga tcagcgtcga cgccgtcgtc accaacctcg ccgtctacat
     3181 agacacgaaa aactccgacg ccctgcgcgc ctgtatgcac cactcgaagc tgctctcggc
     3241 caaggccggc gtgttggccg aggggctatc gtacgacgag gacgccatca tcgagcgcaa
     3301 cctcgcggtg ttgctgcgcc gcctgatcga ggagagcgag gctgctatcc gcgccaagcg
     3361 aggccgcaac attccagagt acgaggcgag gtatcaggac gtgaggatgc tcgcctcgtt
     3421 catccgcgac cgggccgacg ccctgtcgct cgaacggctc ggcctcaacc tggacgcgtt
     3481 cgagtccctg gccaaggatg gcggccgggc cgccaccctg cttggcgcga tgctcgcctc
     3541 ggcaatagcc ttcgccgtcg ccatagtctt gagctactcg ctcaagctga ccgagccgct
     3601 cgacgcgctg gccgccgcgg ccggtaggat atcgaccgga gacttctccg gcggcccgct
     3661 ggcggcctcg gctgacgacg agataggcca gaccgcccga gccttcaacg tgatgcaggc
     3721 gagcatagcc gcctatatag acgaaatacg gaacaaggcc gcggtcgagt cgtcgctgat
     3781 ggaggagcgc ctcaagaacc tcgagatgca gggtcaccta cgcagcgccg agctcaaggc
     3841 gctgcagtcg aggatcaacc cgcacttcct gttcaacacg ctgaacgcgg gcatccagct
     3901 ggcgaccgtc gaggacgcgg accgcacgcg cgtgttcctc gagcgcctcg cggggctcat
     3961 gcgctacagt ttccgcgacc tggacgcgcc ggtggcgctg cgcgacgagc tggcctgcct
     4021 gcgcgactac gcctacctga tgaccatacg cttccccgag tcgatcgact tcgacatctc
     4081 ggccgaaccc ggggcggagg cggccgcctt gccgaagatg atcatccagc cgctcgtcga
     4141 gaactccata cgccacggtt tcgccgataa gccgctcggc ggcgcggtac gcgtgcgggc
     4201 gagcctcgac ggcggagacc tcgtcgtcga ggtagaggac aacgggtcgg gcatcccgga
     4261 ctcccgagcc gccgagatcg tcgctgcggc cgacgccggc accgagctcg gttccgccga
     4321 gggaggcctc ggcatggcta acgtgatcgc aaggctcagg ctgttcgccg gcaggcggga
     4381 ggtgatcgag ttcgcgaacg cggtgccggg cgccagggtg gtcgtgcgcg taccgttcaa
     4441 ggaggcgttc tgatgacggc caagatattc atagcggacg acgagcgacc ggtcatcgat
     4501 ggcgtcgcgg ccgcgatccg gaagcgtttg cccgacgcgg acatctgcgg caccgccggc
     4561 accgggcgag acgcggtgga cggcgccgtc cgggagcgtc ccgacgtgat cctgatggac
     4621 gtgcgcatgc ccggtatgaa cggcctcgag gccctacgcg agctacgctc gtcggcgccc
     4681 gagacggtgc ccatactgct gaccgcctac gagcgcttcg acgtcgccaa ggaggccttc
     4741 ggccttggcg tctacgacta cctggtcaag ccggtcgatc aggaccggct cgtcgaggca
     4801 attcgcggag ctctcgacgc cgccgccgcc aggaaggcag aggcggcgcg cgccaggaac
     4861 gcgatcgagg ccctcgaatc caggcggccc ctcgtcgagg agggcctcgt ctacgcggcc
     4921 ctatccggag actcgggcgg cgctaggatc ctggactatc tctccgcgct cggcttcggc
     4981 cccgagggcg gcgacttcgt cgccgtgagg cggagcgatt ccgggcggcg accgagcgac
     5041 gacgacgagc gcgcgcttcg ggcgtcgctc ggctaccacg cggcctgcgc ggtcggagcg
     5101 ccggcctgcg gcgtgatacc ggtcttctat cccgagtcgg aatccgcctc gatcgagggg
     5161 gccctgctga cggcgctcca ggaaccggag ctacgaggca tgaccgccaa cataggcacc
     5221 tgccgccccc gcgaccggct cgcggaggcc tggcgggaag cgctcgccgg cctggcccgc
     5281 gcgaccgacg gttcggtgtc gcattcgcgc tccatcggcg tcgctacccc gcgttcccgg
     5341 gagccgctcc aagcggccct cgaggccgcc accgtcggcg acgccgccgc ggcctcggcc
     5401 gcgtgggcag cgctaccgga tgaccgcttg ctcagggccg ccgcggccgg ggccatagcg
     5461 tggtcgctcc gccacgaccc ggaagccgtc gtcgaggcgg ccgcggccgt atccgagggc
     5521 gcggcgccga gggccgcctt caactcgggt aagcctggct ccggctacgg tgccaccggc
     5581 cgcgtagccg acgccctgcg gtgcatcgag gagcgctacg ccgaacagat atcgctcgag
     5641 gacgtggcga cccaagtcgg cctctcggcc acccacctgt cgagggtact atcgaacgag
     5701 acgggaaaat cgttcataga gcatctatcc gattacagga tagccagggc caaggcggag
     5761 ctggcctcgg gccgcatgag cgtcaaggag gtcggagccg ccgtaggcta cctcgacccg
     5821 aactacttca gccgggcctt caagcgcgtc accggtctga cgccgtccga ctatgccaag
     5881 tctgccggtc ggggtgacat atgatcggcg ggggaaccaa aggcgcggcc gcgaggcggc
     5941 ggcgagcgat catggccgcg gcggccctag gcgtggcggt cctaggcgtg gcggctatag
     6001 cgctcggact ggccctgagg cagggagcgg cgcggcgctc cagggagccc aagcctgacg
     6061 gggcgggcat atcgatagga ttctccatgg attcgttcgt cgaggaacgc cgcgcccgcg
     6121 accgcgacgt cttcgtcgcc atggcgcgcg accttggagc cacggtcaac gtgcagatcg
     6181 gcgacgagga tccgtccatc caggaggaac agatactgtt cctgctcgat tccggcgtcg
     6241 acgttctggt cgtcataccg agcgaccccg ccggtctcgg ccgggccgtc gccgaggcca
     6301 agcgtagagg cgtcccgatc ctcgcctacg acaggctcat cctgaacggc aacgtcgacc
     6361 tgttcctggc cttcgatgca gaacggtccg gagagctcca ggccgaggcc ttcctggcgt
     6421 ctcagggcgg cgggagggta gcgctctaca acggcaggcg atccgacccg aacgccgcgg
     6481 ccatgcgggc cggcgtcatg aataccctga ggccggacat cgacgagggc cgggtcatcg
     6541 tcgtcgccga ctacgagtcg gctgagcgta ggagcgagga agcctaccag atcgtcgact
     6601 cgatgcttga ggcgggcacg accttcgacg cggtactcgc ggtcgacgat atggccgccg
     6661 aggcgatcat ccgcgccctg tcggtgcggc ggctagccgg gtcggtcttc gtggccggcg
     6721 cggatgccga cctcgcggcc tgtcaacgta tcgcggaggg cacgcagagc atgacggtct
     6781 acaagccgat agaccagata gcggccaagg cggccgagct cgccgtctac ctggccaggg
     6841 gcgacagggt gaccgtgcac aacgccatct gggacggctc ctaccgcatc cccttctacg
     6901 agctggagcc ggtatccgtc acggccgcgg agctgatggc caccgtcgtc aaggacggct
     6961 tccaccttga ggaggacgtg taccgcaacg tgctcgatcg ctaactggat tcttctcacg
     7021 agctcgatcg caatcgtgct atctatatgc agtaacattc cggatgaaac cagtttatcc
     7081 agcagaaaga aaaaactgtc ctgtatcgcg tttttaatcg acgatatacc ctgatattca
     7141 cgggccgtac ggcccggcac tatcatagga ggtactcatc gtgaagaagt cattcgcgat
     7201 cctggctacc ctcgtcgtcg tcggcgcgct cctcgcgtcc tgcggcggct cggccgacgc
     7261 aggcggcacg cccaagatcg gcgtagccat ctacaagttc gacgacacgt tcatgtccta
     7321 cgtccgcaac gccatcgagg ccaacgccac cggcaaggcc accatcgaga tggtggactc
     7381 ccagaacgcc cagccgaccc agaacgacca ggtcgacgcg ttcctgtcca agaaggccaa
     7441 ggtcatcgcg atcaaccccg tcgaccgcac cgccgccgcc gcgatcatcg acaaggccaa
     7501 gggccgctcg acgccggtcg tcttcttcaa ccgcgagccg ctgcccgagg acatgaagaa
     7561 gtgggataag gtttactacg ttggcgccaa ggccgagcag tccggctcga tgcagggcga
     7621 gatcgtcgtc gactacttca aggccaatcc caaggccgac aagaacggcg acggcgtcat
     7681 ccagtacatc atgctcaagg gcgagccggg ccaccaggac gccgagctcc gcaccgagtt
     7741 ctccatcaag gccgtcaccg ccgccggcct gaaggtcgag aagctggccg aggataccgc
     7801 gtattgggac cgcccgaagg ccgtcgagaa gatgaaggcc ttctacgcca agttcggcga
     7861 ccgcatcgag gtcgtgctct gcaacaacga cgacatggcc ctcggcgtca tcgaggccct
     7921 gcgccaggaa ggcttcttct ccggcgacaa gtacctcccg gtagtcggcg tcgacgccac
     7981 cgccccggcc ctccaggccc tcgagcaggg caccctgctc ggcaccgtcc tcaacgacgc
     8041 caagaaccag ggcaaggcca ccttcgacat cgcctacgcg ctggccaccg gcaaggatcc
     8101 cgcccaggcc ggctggacca tctccgacgg caagtacgtc tgggtcccgt accagaaggt
     8161 caccaaggac aactacaccc agttcaaata agctgtaacg cctaaggcgt ttacgcttag
     8221 gacgaacgaa gaggaaaaga gatagaagag ggaagcaaga gaagtcaagg aatttgggag
     8281 aaggcgctat ccggctcccc tccttctcct tggcttcctc cctctttcca atcccttatc
     8341 ctcttatact cttcgttatc ttttccctag tt
//
LOCUS       DAOPHV010000040         8354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_10775_length_8354_cov_4.598626, whole genome shotgun sequence.
ACCESSION   DAOPHV010000040 DAOPHV010000000
VERSION     DAOPHV010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8354
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10775_length_8354_cov_4.598626"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PLQ29_01945"
     CDS             complement(<1..769)
                     /locus_tag="PLQ29_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPG85433.1"
                     /translation="MTAPDDIFRASFVQARQAMIVLDERGIPALWNAAFEELFTELAG
                     FVPERLAVPLFDWLEERESFGFSYYVTEVLHGRMGKAVVETGARTATGRRLWLRTTLS
                     PLRLDGDGRWIWCSFQDVTEQKLKERELVSAKEEAEKATQTKSQFLANMSHEIRTPIQ
                     TILGMTELLGETSLDREQADYIRTVRFSADVLLGLINDVLDFSKIEAGKLDIEAADFD
                     LRATLRQAVDLIILEAHKKGLEVLVDVDEALPEVVHGD"
     gene            complement(800..2083)
                     /locus_tag="PLQ29_01950"
     CDS             complement(800..2083)
                     /locus_tag="PLQ29_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPG85434.1"
                     /translation="MAKASVLIIDESDLFRDYLKARLSRAGLVATAAINGLDGISKMR
                     DDPPDLVVMDYHLTRKTCQEVLEEKARNPNTASVPVVLTAHKIDKNRLLELVRYNIRK
                     VFMKPIKMDGFYQIVSEITGARFEVDKTPCIVEAHVNDNIVFVELAKGINLEKVDLLR
                     FKIRELLDLYAIQKPRVLVMLSDMELSFVDGPNLEALLETIAEASRAKPRHIKVLTRS
                     SFAKEYVSGQPRFAEIEVVSSLEYALDGLVAEAARAADAPDDKAAIITDRILSAQGSA
                     SGGESVEMRFEAERVEAPRLSIESARHLGDGLSIAVVDDDFVIQELLKTTFEAIDAKV
                     HTFPNGRAFLTAVRSRVFDLVFLDLRMPEVDGFGVLAELSAQDLEMPIIVLSAVTQRE
                     SVVRAFKAGVKSYLVKPLKPEQILRKTLEILKANF"
     gene            complement(2085..3416)
                     /locus_tag="PLQ29_01955"
     CDS             complement(2085..3416)
                     /locus_tag="PLQ29_01955"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPG85435.1"
                     /translation="MSDDINFTVPDLGARTVRSPLAFSLSSGDAIANYVDDADRVRWN
                     SSARVSGDAEAAANLLEKAGPRERLYFNPAHVHAGVVTCGGLCPGLNDVIRALVRCLW
                     NRYGVRRVSGVRFGYKGFLPEYGLPVKELTPDVVDDIHKIGGTILGSSRGGGERTAEI
                     VDAIERMNLNVLFAIGGDGTQKGAMAIADELERRRLKVAVVGIPKTIDNDLLFVDRSF
                     GFETAVERAADAVTAAHAEAHSSINGVGLVKLMGRDSGFIAVHTALATHEANFVLIPE
                     APFDLGGPNGLLAHLERRLQARNHAVIVVAEGAGQDAIALDPSATDASGNRRLGDVGA
                     WLKDAITAHFKARGLELNLKYIDPSYMIRSAPACPTDSVYCERLGNNAVHAAMAGKTR
                     VLIGMVNNEFVHLPTGVVVTRRNTVDPEGSLYRDAIDATGQPVSMRNAREA"
     gene            3641..>8354
                     /locus_tag="PLQ29_01960"
     CDS             3641..>8354
                     /locus_tag="PLQ29_01960"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPG85436.1"
                     /translation="MGKRAYCILVALFACALSAPIVAQDAPAAKPATEYQGADGLENW
                     TTEYDVSGLKPGTYNLLARAIDAAGNVTFAVPFDLIVDPASDLPIAGIVNPLHLSRVG
                     ADLNVVGTCVDDDAVSRVELRLDDGEWVRAVGSDYWSYYLPTAGVADGLHTISARGVD
                     SNGLVGGESTVSFHLDRTKPLHEVAKPAFGAIASGRLAVTGTVYDANGLSSVSYSSDS
                     GATWLPLRHSYDKKTRTASFSLAVDTRKLDDGPAVLWLKSVDAVGSEGAAVFLYFVDN
                     TVPELVALSPAEGEAVNGSFELRGRVYDTVGLQSLSWAYGKETGAVELVPGNPYFSIP
                     FTAPAKPGPMTMKLTATDVAGNRAVVGVTRRVDPAADLPTVSVAFPAPEASLEGTVRV
                     VGMARDDDGVASVAWSLDGGPETTIATAGAFSLELGSVPSGRRVLALRAVDANGLAGP
                     WLRLPFVYSGAAPAVALERAADASGSRDFAPGVSVSTIDGKASLQGSVRAANPLVELS
                     YTINGGAPMKLPFSKTADGASFALPLSASLPYGVLDLRVEAVDSFGRRGAARAPILAI
                     DYSKARVGPLLDFGLAAEPVSAPAGSALAADEARTVILRGGAPLVGSFVAPFGGEAPR
                     SVRLDPPTSLVEASIDGMFIRVERAADARTAPTAVVVETERGHVFSEGPFIFRTDGVP
                     PAVTIVEPAFGSWLRGRIGLRARATDGDEVASIEYAIDGGDWKPLEAGAAGTGDYGAS
                     IDLTGLEGPVRLELRAVDPSGNASTVSTAFMADSAPPAPVRLLPRPGDRPSGPTRFAF
                     EAGESLRSLGLVELDSGSGYERLEPSALITFTADPRAALRLRLTDRAGNATELNPADG
                     LAPADTPAAAPQPLESIKSNVAALPGTPEGPMALFSGSDANGALSFGSPMVREPAEAA
                     FPDYAKAPIRASGAASLSIAFSGVSPDPKKPLAFWGYGPGELTQPLALKKGAAPDLWT
                     AAVKLAPRPDGRSDVWVAVTEASGELAYARAALEYDSTPPAIEILAPAAVDGKAATPG
                     AFTLVVRADDVAGIASLSYASGSESGEFERAPGSGDAAKRFTFPPKAGGATIHVRAVD
                     GSGNRSQTTLTVAYDAVADAPKVRFLSPAAGAELDAGPAEAFIYATDDDLLASATLVV
                     DGATVEASGPGPLYSLALGEAKRGQRSAQAWATDSDGVQSAKATIAFVQRAAAPILSF
                     TSAIAPVAGAAPEPSAFQAGSALVADGKTILVASAMAANGLAKAEWALNDGAWSSLPI
                     PKPAADGSYELRLALPAGLAYERNLVRLRVSDAFGQAAEAETSFYRVAPERDLERTAG
                     EGVYLYDERVADGRVTLAPGLAIGALWYGRPVASLAVEPPVPYAEASFDGRALRLTGL
                     ADGGVGAEPVVLVVETVDGERFRSSPLGLVVDSGPPALTITEPASGSPTTGSWRLAGE
                     ASDPNGLAELAWSADGGLSWNRLEPGKSASYSAELSPEAIDGPVGLIVRAVDRAGAAS
                     YALTAVARDTAAPAIVFETPRSVDTVNGTVLLAGYAEDALSVAGVAYSPDGLAWETLD
                     GEPRGAAAEPYAPARQSFS"
BASE COUNT         1215 a         2948 c         2880 g         1311 t
ORIGIN      
        1 ggtcgccgtg gacgacctcg ggcagcgcct cgtcgacgtc gaccagcacc tcgaggccct
       61 tcttgtgcgc ctccaggatg atcaggtcga ccgcctgcct gagcgtggct cgcaggtcga
      121 agtcggcggc ctcgatatcg agcttccccg cctcgatctt ggagaagtcg agcacgtcgt
      181 tgatcaggcc gagcagcacg tcggccgaga accgtaccgt gcggatatag tcggcctgct
      241 cccggtcgag cgaggtctcg ccgagcagct cggtcatgcc caggatggtc tggatgggcg
      301 tgcgtatctc gtggctcatg ttggccagga actggctctt ggtctgggtg gctttctcgg
      361 cctcttcctt ggccgagacg agctcgcgct ccttgagctt ctgctcggtg acgtcctgga
      421 acgagcacca gatccagcgt ccgtcgccgt cgagccggag cggcgacagg gtggtccgta
      481 gccagaggcg gcggcccgtg gccgtgcgcg cgccggtctc gacgacggcc ttgcccatgc
      541 ggccgtgcag cacctcggtc acgtaatacg agaagccgaa ggattcgcgc tcctcgagcc
      601 agtcgaacag cggcaccgcc agccgttcgg gcacgaaacc ggccagctcg gtgaacagct
      661 cctcgaacgc ggcgttccag agggcgggta tgccccgctc gtcgagcacg atcatggcct
      721 gcctggcctg gacgaacgag gcgcggaaga tgtcgtcggg cgctgtcatg atcgctcctg
      781 cgccgccacg ggcgactcgc tagaaattgg ccttgagtat ctccagggtc ttcctgagga
      841 tctgctcggg cttgagcggc ttgaccaggt agctcttgac gccggccttg aaggccctga
      901 ccaccgactc gcgctgggtc accgcggaca gcacgatgat cggcatctcg aggtcctggg
      961 cggacagctc ggcgagcacc ccgaacccgt ccacctccgg catgcgcagg tcgaggaaga
     1021 ccaggtcgaa caccctcgag cgcaccgcgg tcaggaacgc cctgccgttc gggaaggtgt
     1081 gcaccttggc gtcgatcgcc tcgaaggtgg tcttgagcag ctcctggatg acgaagtcgt
     1141 cgtcgaccac ggctatcgac aggccgtcgc cgaggtgcct ggccgactcg atcgacagcc
     1201 gcggcgcctc gacgcgctcc gcctcgaagc gcatctccac cgactctccg ccggacgccg
     1261 agccctgggc cgacaggatg cggtcggtga tgatagcagc cttgtcgtcg ggggcgtccg
     1321 cggccctcgc cgcctccgcg accagcccgt cgagggcgta ctcgaggctc gagaccacct
     1381 cgatctcggc gaagcgcggc tggccggaga cgtactcctt ggcgaagctc gagcgggtca
     1441 gcaccttgat gtgcctgggc ttggcgcgcg acgcctcggc gatcgtctcg agcagggcct
     1501 cgaggttcgg cccgtcgacg aagctcagct ccatgtcgga gagcatgacg agcacgcggg
     1561 gcttctggat ggcgtagagg tcgagcagct cgcggatctt gaagcgcagg aggtcgacct
     1621 tctcgaggtt gatccccttg gccagctcga cgaacacgat gttgtcgttc acgtgggcct
     1681 cgacgatgca gggcgtcttg tcgacctcga agcgggcgcc ggtgatctcg gagacgatct
     1741 ggtagaagcc gtccatcttg atcggcttca tgaacacctt gcgtatgttg tagcggacca
     1801 gctcgagcag gcggttcttg tctatcttgt gggcggtgag cacgacgggg acggacgcgg
     1861 tgttcgggtt gcgcgccttc tcctcgagca cctcctggca ggtcttgcgc gtcaggtggt
     1921 agtccatgac gacgaggtcg ggcgggtcgt cgcgcatctt cgagatgccg tcgaggccgt
     1981 tgatcgccgc ggtggccacg aggccggccc ggctcagcct ggccttgaga tagtcgcgga
     2041 acaggtccga ctcgtcgatg atgagcacgc tggccttggc cacgctacgc ctcccgcgcg
     2101 ttgcgcatcg agacgggctg gccggtcgcg tcgatggcgt cgcggtacag gctgccctcc
     2161 ggatccaccg tgttgcgccg ggtcacgacg acgccggtgg gcagatggac gaactcattg
     2221 ttgaccatgc cgatcaggac gcgggtcttg ccggccatag cggcgtggac cgcgttgttg
     2281 cccaggcgct cgcagtagac cgagtcggtc gggcaggccg gagcgctccg tatcatgtag
     2341 ctcgggtcga tgtacttgag gttcagttcg aggccgcgcg ccttgaagtg ggccgtgatg
     2401 gcatccttga gccaagcgcc gacgtcgccg aggcgccggt tgcccgaggc gtcggtcgcc
     2461 gacgggtcca aggcgatcgc gtcctggccg gctccctccg ccacgacgat gacggcgtgg
     2521 ttcctggcct ggagcctgcg ctccaggtgc gcgagcagcc cgttcgggcc gcccaggtcg
     2581 aacggcgcct cggggatcag cacgaagttg gcctcgtggg tggcgagcgc cgtatgcacc
     2641 gcgatgaagc ccgagtcgcg gcccatcagc ttgacgagcc cgacgccgtt gatcgacgag
     2701 tgggcctcgg cgtgggcggc cgtcaccgcg tcggcggccc gctcgacggc cgtctcgaaa
     2761 ccgaagctac ggtccacgaa caggaggtcg ttgtcgatgg tcttgggtat gccgacgacg
     2821 gctaccttga gcctgcgccg ctcgagctcg tcggctatgg ccatcgcgcc cttctgcgtg
     2881 ccatcgccgc cgatggcgaa cagcacgttc aggttcatgc gctctatcgc gtcgactatc
     2941 tcggcggtgc gctccccgcc gccgcgcgac gagccgagta tggtgccgcc tatcttatgg
     3001 atatcgtcga cgacgtcggg cgtcagctcc ttgaccggca ggccgtactc gggcaggaaa
     3061 cccttgtagc cgaagcgcac gccggagacg cgcctgacgc cgtagcggtt ccacaggcag
     3121 cgtaccaggg cgcggatcac gtcgttgagc cccgggcaaa ggccgccgca ggtcacgacg
     3181 ccggcgtgta cgtgcgccgg gttgaagtac agccgctccc tgggcccggc cttctcgagg
     3241 aggttcgccg ccgcctcggc gtcgccggag acgcgggcgc tcgaattcca gcgcacccgg
     3301 tcggcgtcgt cgacgtagtt ggcgatggcg tcgcccgagg acagcgaaaa ggccagcggc
     3361 gagcgtacgg tccgcgcgcc gagatccgga acggtgaagt ttatatcgtc ggacatgtcc
     3421 catccttaga accgggcgcc aaggaggccg agagacgggc ggatccggtt tcacgctggc
     3481 agagagtata gcggataagc actcctttct caatcgaaag aactagcacg gtttatacat
     3541 atttgtgcta caatttcgtc gcatcgatgt agtatgtaac ccatggtagc tgctgccgat
     3601 actagaggca gacacatccg ctcgttccag ggagtccgtc atgggtaaga gggcatactg
     3661 catactcgtc gctctgttcg cctgcgctct atcggcaccg atcgtcgcgc aggacgctcc
     3721 ggccgccaaa ccggctacgg agtaccaggg cgccgacggg ctcgagaact ggacgaccga
     3781 atacgacgta tcgggactca agccaggcac gtacaacctc ctggccaggg cgatcgacgc
     3841 ggccgggaac gtgaccttcg ccgtcccgtt cgaccttatc gtcgatcctg cctccgacct
     3901 gccgatagcc ggcatcgtca acccgctcca tctctcccgg gtcggcgccg acctgaacgt
     3961 cgtcggtacc tgcgtcgacg acgacgcggt gagccgcgtg gagctcaggc tcgacgacgg
     4021 cgagtgggtg cgggcggtcg gctccgacta ctggtcgtac tacctgccca ccgccggcgt
     4081 ggccgacggg ctccatacca tctcggcccg cggcgtcgac tccaacggcc tggtcggcgg
     4141 ggagagcacg gtctccttcc acctggaccg gaccaagccc ctccacgagg tggccaagcc
     4201 ggcgttcggg gccatagcgt cggggcgact ggcggtgacc ggcacggtct acgacgccaa
     4261 cggcctgtcg tcggtatcct actcgagcga ctccggcgcg acctggctac cgctgcgcca
     4321 ttcgtacgac aagaagacca ggacggcctc cttctcgctc gccgtcgaca cgaggaagct
     4381 cgacgacggc ccggcggtgc tctggctcaa gagcgtcgac gccgtcggct ccgagggcgc
     4441 cgccgtcttc ctgtacttcg tcgacaacac cgtaccggaa ctggtagcgc tgtcgccggc
     4501 cgagggcgag gccgtcaacg gcagcttcga gctgcggggc cgggtctacg ataccgtggg
     4561 cctccagagc ctgtcctggg cctacggcaa ggaaaccggc gcggtggagc tcgtgcccgg
     4621 caacccgtac ttctccatac cgttcacggc tcccgccaag cccggcccga tgaccatgaa
     4681 gctgacggcg accgacgtgg ccgggaaccg ggccgtcgtc ggcgtgaccc gccgcgtcga
     4741 cccggcggcc gatctgccga ccgtctccgt ggcgttcccg gcgccggaag cctcgctcga
     4801 ggggaccgtg cgcgtcgtgg gcatggccag ggacgacgac ggcgtcgcgt cggtggcctg
     4861 gagcctcgat ggcggcccgg agacgaccat tgcgacggcc ggagcgttct cgctcgagct
     4921 cggctcggta ccgagcggcc ggcgcgtcct ggccctacgg gccgtggacg cgaacggcct
     4981 ggccgggccc tggctcaggc tgcccttcgt ctactcggga gcggccccgg ccgtggcgct
     5041 cgagcgtgcg gccgacgcct ccggttcgcg ggacttcgcg ccgggcgtct cggtatcgac
     5101 gatagacggc aaagcctcgc tacaaggctc ggtgcgggcg gccaatcccc tcgtcgagct
     5161 atcctatacg atcaacggcg gcgcgcctat gaagctgccg ttctccaaga cggcggacgg
     5221 ggcgagcttc gccctgcccc tctcggcgtc gctcccctat ggcgtgctcg acctgcgcgt
     5281 cgaggcggtc gactcgttcg gtaggcgcgg cgcggcccgc gcgccgatcc tggcgatcga
     5341 ctactcgaag gcgcgggtcg gcccgctcct cgacttcggc ctggccgccg agcccgtctc
     5401 ggcgcccgcc ggctcggccc tcgcggccga cgaggcgcgg acggtgatcc tgcgaggcgg
     5461 cgcgcccctc gtcggctcct tcgtcgcccc gttcggcggc gaagccccgc gctcggtcag
     5521 gctcgacccg ccgacaagcc tcgtcgaggc gtccatagac ggcatgttca tacgcgtcga
     5581 gcgcgcggcg gacgcccgga cggccccgac cgcggtggtc gtggagaccg agcgcgggca
     5641 cgttttctcc gagggaccgt tcatattccg gaccgacggg gtgccgccgg ccgtgaccat
     5701 cgtcgaaccg gccttcggct catggttacg cggccgcata ggcctgcgcg cccgggcgac
     5761 cgacggcgac gaggtcgcct cgatcgagta cgccatcgac ggcggcgact ggaagccgct
     5821 cgaggccgga gcggcgggga cgggcgacta tggcgcctcg atcgatctga cgggcctcga
     5881 gggcccggtt cgcctggagc tgcgcgccgt cgacccgtcg ggtaacgcgt cgacggtatc
     5941 gaccgccttc atggccgact cggcgcctcc cgcgccggtc aggctcctgc cgcggccggg
     6001 cgaccggcca tccggcccca cccggttcgc gttcgaggcc ggcgaaagcc tccgatcgct
     6061 cgggctcgtc gagctggact cgggctcagg ctacgagcgg ctcgagccgt ccgcgctgat
     6121 cacgttcacc gccgatccga gagccgccct ccgcctcagg ctgaccgacc gcgccggtaa
     6181 cgcgaccgag ctgaacccgg ccgacgggct cgcgccggcc gacaccccgg ccgcggcgcc
     6241 gcagccgctc gagtccatca aatcgaacgt ggccgccctg ccgggaaccc cggaaggccc
     6301 gatggcgcta ttcagcggct ccgacgcgaa cggcgcgcta tcgttcggct cacccatggt
     6361 gcgggaaccc gccgaggcgg ccttccccga ctacgccaag gccccgatcc gagcctcggg
     6421 cgcggcgtcg ctgtcgatag ccttctcggg cgtctcgccc gatcccaaaa agccgttggc
     6481 cttctggggc tacggccccg gagagctgac ccagccgctc gccctcaaga aaggcgccgc
     6541 gccggacctg tggacggccg ccgtcaaact ggcgcccagg cccgacggcc gaagcgacgt
     6601 ttgggtcgcc gtcaccgagg cttccggcga gctggcctac gccagggcgg ccctcgagta
     6661 cgactcgaca ccgccggcca tcgagatact ggctccggcc gccgtagacg gcaaggccgc
     6721 cacgcccggc gccttcacgc tggtcgtccg ggccgacgac gtcgcgggca tcgcctcgct
     6781 gtcgtacgcc tcaggctcgg agtcgggcga gttcgagcgc gcgcccggct cgggcgacgc
     6841 cgccaagcgc ttcaccttcc cgcccaaggc cggcggggcg acaatacacg tacgagccgt
     6901 cgatggctcc ggcaataggt cccagacgac gctcaccgtg gcctacgacg ccgtcgcgga
     6961 cgcgcccaag gtccggttcc tgagccccgc cgccggcgcc gaactcgacg ccgggccggc
     7021 cgaggccttc atctatgcga ccgacgacga cctccttgcg agcgcgacgc tcgtcgtcga
     7081 cggcgccacg gtcgaggcga gcggcccagg cccgctctac tcgctcgctc tcggcgaggc
     7141 caagcgcggc cagcgctcgg cccaggcctg ggcgaccgac tcggacggcg tccagtcggc
     7201 caaggcgacc atagccttcg tccagcgcgc agccgcgccg atactttcct ttacgtcggc
     7261 catagcgccg gtcgccggcg cggcgcccga gccttcggcg ttccaggccg gctcggccct
     7321 ggtagccgac ggcaagacca tcctcgtcgc gtcggccatg gccgcgaacg gactggccaa
     7381 ggccgagtgg gccctgaacg acggcgcctg gtcatcgcta cccatcccga aaccggcggc
     7441 cgacggctcg tacgagctac ggctggcctt gcccgccggc ctcgcgtacg agcggaacct
     7501 ggtgcgtctc agggttagcg acgccttcgg ccaagccgcc gaggccgaga cctcgttcta
     7561 tcgcgtcgcg ccggagcgtg acctcgagcg gacggccggc gagggcgtct acctctacga
     7621 cgagcgcgtc gcggacggtc gcgtgaccct agcgcccggc ctggcgatag gcgcgctctg
     7681 gtacggcagg ccggtcgcga gcctggccgt cgagccgccc gtcccctacg cggaggcttc
     7741 cttcgacggc cgggccctgc gcctgaccgg cctggccgac ggcggcgtcg gcgccgaacc
     7801 ggtggtcctg gtcgtcgaaa cggtcgacgg cgagcgcttc cgctcctcgc cgctcgggct
     7861 ggtagtcgat tctggcccgc cggcgttgac cataaccgag ccggcctcgg gaagcccgac
     7921 gaccggctcg tggcgcctgg ccggcgaggc ctccgacccg aacggcctgg ccgagctggc
     7981 ctggtccgcc gatggcggcc tgagctggaa ccggctagaa ccgggcaaaa gcgcgtcgta
     8041 ctccgccgag ctatcgccgg aggcaatcga cgggccggtc ggcctcatcg tgcgggccgt
     8101 cgaccgggcg ggcgccgcga gctacgcgct gacggccgtg gcccgcgata ccgccgcgcc
     8161 ggccatcgtc ttcgagacgc cgcgatcggt cgataccgtc aacggcaccg tgctgctcgc
     8221 gggctacgcg gaggacgcgc tctcggtcgc cggcgtcgca tacagccccg acggcctcgc
     8281 ctgggagacg ctcgacggcg agccccgcgg agcggccgcc gagccgtacg ctccggcgag
     8341 gcaatcgttc tcgc
//
LOCUS       DAOPHV010000041         8320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_10832_length_8320_cov_3.622384, whole genome shotgun sequence.
ACCESSION   DAOPHV010000041 DAOPHV010000000
VERSION     DAOPHV010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10832_length_8320_cov_3.622384"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PLQ29_01965"
     CDS             <1..516
                     /locus_tag="PLQ29_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPG85437.1"
                     /translation="ERYQAGFEQGVRAVYPEARILVDYAGDFAAPDTGQALARKQFNA
                     GAYVIYHASGGTGNGMIQEARDRSARGDLRWAIGVDKDQYSDGLYGGKSAVLTSMMKR
                     VDVAAYEVAKMTLAGQFPGGQTLVFSLKNNGVGIPNENPNLSADIVASVNEYAKKIAS
                     GELVVSEIPAK"
     gene            789..2309
                     /locus_tag="PLQ29_01970"
     CDS             789..2309
                     /locus_tag="PLQ29_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG85438.1"
                     /translation="MAFIIEMLNIVKDFPGIRANDDVTLQVEKGSIHALLGENGAGKS
                     TLMSILFGLYSPDGGSIRIRGETVRISDPNVANELGIGMVHQHFKLVHNFTVTENIIL
                     GLEPKKGPVLDIKSAAVKVAELSKLYGLDVDPFAKIEDISVSMQQRVEILKMLYRDAE
                     ILIFDEPTAVLTPQEIHELMGIMRRLVEEGKTILLITHKLKEIKAVADTCTVLRRGKL
                     IGTVPVAGTSEASMAEMMVGREVSFSVDKGPSHPGEVVLDIKDLCVKNAKGLLGVKNL
                     SLSVREGEILGLCGIDGNGQTELIQALTGLAPIESGSVTVRGVDITDLPVKQRLDSGL
                     GHIPEDRHRHGLVLDFRLDENMVIHSYGSEPFSRHGILDYGAIRKNAERLIDEFDVRS
                     GRGADTKARSMSGGNQQKAILAREIDRSPIAFVIAQPTRGLDVGAIEYIHKRIVAERD
                     KGKAILLASLELDEILDVSDRIAVIYGGEIVGVVDADKTDENELGLMMSGSVRKAG"
     gene            2311..3441
                     /locus_tag="PLQ29_01975"
     CDS             2311..3441
                     /locus_tag="PLQ29_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG85439.1"
                     /translation="MANRKLGDARKGDALLVPIVAVLLGFVVGSVVVMITGKSPLVMF
                     NAMLQGLTGLNVARGSFNPRYIGELVIQAMPIILTALCFGFAARTGLFNIGAEGQLMI
                     GSLAATTVALLVKAPAFIHIPLVLVAGMAAGALWGAIPGYLKARFNVHEVVVTIMLNY
                     VALHMNNLLILNVFGSFDKVKTAPFPQSALLKDPWLESITNGSRLNWGFIPVLIAIVV
                     YWFVIEKTTFGYGLRAVGYNKDAAKYAGMKVNRNIVLSMMIAGAFAGLAGAVITTGTF
                     NFGRALSAAEGYGFDGMAVALVGSNQALGILLAGLLFGMLKAAGPLMQSNGIPKEIGG
                     IIQASIVLFVAMKYGLERLIAMLRLRRDKASSLPVTGEEAGV"
     gene            3438..4391
                     /locus_tag="PLQ29_01980"
     CDS             3438..4391
                     /locus_tag="PLQ29_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG85440.1"
                     /translation="MNLGMILEMFPIALMFGAPIVMAALGGLFSERSGVTNIALEGIM
                     MVGAFAGASVTVVLEPVTAMAPWLGLLTGIAAGMLFSLLHAFTSIHLKADQIISGTAL
                     NILASGLTIYLCQILFNQQRTRAFSYGLRKVTVPILSKIPIIGPMLFYEMYPTFYLAI
                     TLVVVTWFVVFKTPFGLRLRSCGEHPQAAASMGINVYAMRYAGVIASGAFAGLAGATM
                     VLTQDIQYTVTSIHGTGFIALASLVFGKWNPWGVLGAGMFFGFSQTLSYYAKDIKILA
                     SLPLEVFYALPYVLTIVALVVFAGKSVGPKAAGEIYDQGKR"
     assembly_gap    4503..4602
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4701..5759)
                     /locus_tag="PLQ29_01985"
     CDS             complement(4701..5759)
                     /locus_tag="PLQ29_01985"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPG85441.1"
                     /translation="MLNALGSVFSVLLMIALGFVLARRSWFSASGYAGGGANGQAVRE
                     RASKDSDGYAGGGANGQAEDFGGKLISRLVVNVSLPAYMIANLMGGYDRARLLSMLPG
                     LPIPFLSMAAAFASSAAVAALFKIRPERRGTFSSMASLSNTIFIGLPVNMMIFGEESL
                     PYVLIYYIANTVMFWTLGVYGISGDGARLAGNPAPRFLSPTGLRRLLSPPLLGLMVAI
                     VLIMLGVDLPKPLLDTARTIGSMTTPLSMLFIGIVIAKVDWRSIRLERDLVLVALCRF
                     VVSPAIVILLLRLTDLPMLMKQVFFVQSMMPAMTQTPILVATYGADIEFAGLGTSMTT
                     MLSLLVIPACLALSPLVL"
     gene            complement(5803..7017)
                     /locus_tag="PLQ29_01990"
     CDS             complement(5803..7017)
                     /locus_tag="PLQ29_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPG85442.1"
                     /translation="MKSTKGEQVWLAAGAASAAMAAFLVWAFAQPGTLGMYRWGYRAP
                     VLGLIALGALPIVAAAAARALSRTKGAAPRIATGIVFAASAVTLLTAISVSAYIFTNA
                     YGRAASPDRPTTIAPSDGIPRRTGADGEPILRVAVSSDGHYGRDASNAEARSAILRLS
                     EVEHRSGGLDAFINLGDIVEMGMEADDWREAIESIRRDAPTLPFLALLGNHDALIGGY
                     ARWQAAFGPKGGSSAPTGPSPSSWRIDAGRVHFIALDLLWGPEGFGAKEKAWLVAQLT
                     AIPPSDYTVVLSHCFFYSSGYIDEETGMAWYDHADMLREVAPLIAGKADLVISGHNHY
                     MEWIEADGTAWAVVGALGGKPDPVPSYVSPGSVWRNQGVFGRLVLELTPDGLACEFQD
                     QDGTPLFNRVLR"
     gene            7534..7809
                     /locus_tag="PLQ29_01995"
     CDS             7534..7809
                     /locus_tag="PLQ29_01995"
                     /inference="COORDINATES: protein motif:HMM:NF040785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HPG85443.1"
                     /translation="MPYLNADGVLPPELLSEIQKYVQGALVYVPRQGPERLGWGQKNG
                     ARDELDRRNAAIREAKACGRTIDELADEYCLSADGIRKILYCNRRAS"
     gene            complement(7804..>8320)
                     /locus_tag="PLQ29_02000"
     CDS             complement(7804..>8320)
                     /locus_tag="PLQ29_02000"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPG85444.1"
                     /translation="KELAERDELTGLRNRRSGANRLALENARSSRSSAPFCVAIGDID
                     HFKAFNDLHGHETGDLVLKHVAGAVERSVRMEDMAARWGGEEFLILLSGDSLPAALEA
                     SDRIRKAVSAEAVTVGTERLHISMTIGVAEFAKGEPIDELIARADAAMYKGKAAGRDI
                     VVAAPPPRARS"
BASE COUNT         1510 a         2581 c         2642 g         1487 t
ORIGIN      
        1 gagagatacc aggcaggatt cgagcagggc gtcagggccg tctacccgga ggccaggata
       61 ctcgtcgact acgccggcga tttcgcagcc ccggatacgg gccaggccct tgcgcggaag
      121 cagttcaacg ccggcgccta cgtcatctac cacgcgtccg gaggcaccgg gaatggtatg
      181 atacaggagg ctcgggatcg ttccgctagg ggcgacctac gttgggccat cggcgtcgac
      241 aaggaccagt actccgacgg cctctacggc ggcaagtccg ccgtgctgac ctcgatgatg
      301 aagcgcgtcg acgtggccgc ctacgaggtc gcgaagatga ccctggctgg ccagttcccc
      361 ggcggccaga ccctcgtgtt ctccctgaag aacaacggcg tcggcatccc gaacgagaac
      421 ccgaacctgt cggccgacat cgtcgcgtcc gtcaacgagt acgccaagaa gatcgcttcc
      481 ggcgagctcg tcgtctcgga aatccccgcg aagtaattcg cctccctatc gcgctatccg
      541 tcgcctccgg gcgccgggta gcgccgacgg tatccgtcac gtgagcggct agtggcgcga
      601 caggcgtacc gatggtgcgc tcgccgcggc tactgggccg ctcttcgttt ctatgacgaa
      661 ggcgcggcgc gtcgtccgcc ggtcgcccga cggttccttc ccaaagagct ttatttatag
      721 tattctgtat gacccgcgcg ggtgatcccg gcgcggaaga atcccagatg acacaatggg
      781 ggcacgcgat ggcgttcatc atcgagatgc tcaatatcgt caaagacttc ccgggcatcc
      841 gcgctaacga cgacgtgacg ctccaggtag agaagggcag catccatgcc cttctgggcg
      901 agaacggcgc cggtaaatcg acgctgatga gcatcctatt cgggctgtat tcgcccgacg
      961 gaggcagcat ccggatccgc ggggagaccg tccgcatcag cgatccgaac gtagccaacg
     1021 agctgggcat cggcatggtg catcagcatt tcaagctcgt acataatttc acggtgaccg
     1081 agaatatcat cctcggcctc gagccaaaga aggggcccgt gctcgatata aagagcgcgg
     1141 ccgtcaaggt cgccgagctg tcgaagctct acggcctgga cgtcgacccc ttcgccaaga
     1201 tcgaggacat cagcgtgagc atgcagcagc gggtcgagat cctcaagatg ctctaccgcg
     1261 acgccgagat cctgatcttc gacgagccta ccgcggtact gacgccgcag gagatccacg
     1321 agctgatggg catcatgcgt cggctcgtcg aggagggcaa gaccatcctc ctgatcaccc
     1381 acaagctcaa ggagatcaag gcggtcgccg atacctgcac cgtgctcagg cgcggcaagc
     1441 tgatcggtac cgtgccggtc gccgggacca gcgaggcgag catggcggag atgatggtcg
     1501 ggcgcgaagt cagcttctcg gtcgacaaag gcccctccca tccgggcgag gtcgtcctcg
     1561 atatcaagga tctatgcgtc aagaacgcca agggcctcct gggcgtcaag aacctgtcgc
     1621 tgagcgtgcg cgagggcgag attctcggcc tgtgcggcat cgatggcaac ggccagaccg
     1681 aactgatcca ggccctgacc ggccttgcgc ccatcgagtc aggctccgtg acggtccggg
     1741 gcgtcgacat caccgacctg ccggtcaagc agcggctcga cagcgggctc ggccatatcc
     1801 ccgaggatag gcatcgccac ggtctcgtgc tcgacttcag gctcgacgag aacatggtca
     1861 tccattccta tggctccgag ccgttctcga ggcacggcat cctggactac ggcgccatac
     1921 gcaagaacgc cgaacgcctg atcgacgagt tcgacgtgcg ctccgggcgc ggcgccgata
     1981 ccaaggcccg ctcgatgtcg ggcggcaacc agcagaaggc gatactcgcg cgcgagatcg
     2041 accggtcgcc catcgccttc gtcatagcgc agccgacgcg cggcctcgac gtgggcgcta
     2101 tcgaatacat ccataagcgc atcgtcgccg agcgcgacaa gggcaaggcg atcctgctcg
     2161 cctcgctgga gctggacgag atactcgacg tgtccgacag gatcgcggtc atctacggcg
     2221 gcgagatcgt cggcgtggtc gacgccgaca agaccgacga gaacgagctc ggcctgatga
     2281 tgtccggctc ggtcaggaag gccggttaac atggcaaaca gaaaactagg cgacgcgagg
     2341 aagggcgacg cgctcttagt accgatcgtc gcggtgctgc tcggcttcgt ggtcggttcg
     2401 gtggtcgtca tgatcaccgg caagagcccc ctcgtcatgt tcaacgcgat gctacagggc
     2461 ctgaccggcc tgaacgtcgc caggggatcg ttcaacccgc gctacatagg cgagctggtc
     2521 atccaggcca tgcctatcat cctgacggcc ctgtgcttcg gcttcgctgc ccgtaccggc
     2581 ctgttcaata tcggcgccga gggccagctg atgatcggct cgctggcggc taccaccgtg
     2641 gcgctgttgg tcaaggcgcc ggccttcatc catattcccc tcgtgctcgt cgccggcatg
     2701 gcagcgggcg cgctgtgggg agcgatcccg ggttacctga aggcgcgttt caacgtgcac
     2761 gaggtcgtcg tgaccatcat gctcaactac gtggccctgc atatgaacaa cctcctcatc
     2821 cttaacgtgt tcggctcgtt cgacaaggtc aagacggccc cgttcccgca gtcggccttg
     2881 ctcaaggatc cctggctcga gtccataacg aacgggtccc gcctgaattg gggcttcata
     2941 ccggtgttga tcgcgatcgt cgtctactgg ttcgtgatcg agaagacgac cttcggctac
     3001 ggtctcaggg ccgtcggcta taacaaggac gcggccaagt acgccggcat gaaggtcaac
     3061 cgcaacatcg tgctgtcgat gatgatagcc ggcgccttcg ccggactcgc cggcgcggtg
     3121 atcaccaccg gcaccttcaa cttcggtagg gcgctgtcgg cggccgaggg ttacggcttc
     3181 gacggcatgg ccgtggcgct cgtcggctcg aaccaggcgc tgggtatcct gctcgcgggc
     3241 ctcctgttcg gcatgctcaa ggccgccggt ccgctgatgc agtcgaacgg catcccgaag
     3301 gagatcggcg gcatcatcca ggcgtcgatc gtcctgttcg tggccatgaa atacggattg
     3361 gagcgcctga tagctatgct cagactgagg cgcgacaaag cgtcctcgct acccgttacc
     3421 ggcgaggagg cgggcgtatg aacctcggca tgatcctaga gatgttcccg atagcgctga
     3481 tgttcggcgc gcccatagtc atggccgccc tcggcggcct gttcagcgag cgctcgggcg
     3541 tgacgaacat agcgctcgag ggcatcatga tggtcggagc cttcgccggc gcgtcggtca
     3601 cggtcgtgct tgaaccggtt acggcgatgg ccccgtggct cggcctcctg accggcatcg
     3661 cggccggcat gctgttctcg ctcctgcacg cgttcacgag catccatctc aaggccgacc
     3721 agatcatctc cggcacggcg ctgaacatac tggcgagcgg tctgaccatt tacctctgcc
     3781 agatcctgtt caaccagcag cgcactcgcg cgttcagcta cggtctgcgc aaggtgacgg
     3841 ttccgatcct atccaagata ccgatcatcg ggccgatgct attctacgag atgtacccga
     3901 cgttctacct cgcgatcacg ctcgtcgtcg tcacctggtt cgtcgtgttc aagacgccgt
     3961 tcgggctccg cctgcgctct tgcggcgagc atccccaagc ggccgcgtcg atgggcatca
     4021 acgtctacgc gatgcgctac gcaggcgtca tcgcctccgg cgccttcgcc ggattggccg
     4081 gggcgacgat ggtactgacc caggatatcc agtacacggt cacctccatc cacggcaccg
     4141 gcttcatcgc tctagcgtcg ctcgtattcg gcaagtggaa cccctggggc gtcctcggcg
     4201 caggcatgtt cttcggcttc tcccagacgc tgtcgtacta cgccaaggac atcaagatat
     4261 tggcgtcctt gccgctcgag gtgttctacg cgctacccta cgtgctgacc atcgtggcgc
     4321 tcgtcgtatt cgccggcaag tccgtggggc cgaaggccgc gggcgagatt tacgatcagg
     4381 gaaagcgtta ggcgatcgcc gacgcttcgc gcggcgatcg ctcccgcggg ttcaaccgga
     4441 ctcgctggcg ctcgccggtt aactccgcgg gccggtacgg cgggtgaccg agctcgctgc
     4501 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntaactccg cgggccggta
     4621 cggcgggtga ccgagctcgc tggcgctcgc cggtcactga aatgtacttg gggaggggga
     4681 tctatgatcc ccctcttttt ttatagcacg aggggagaga gggctaggca ggctggtatg
     4741 acgagcaggc tgagcatggt cgtcatcgag gtgccaaggc ctgcgaactc gatgtcggcg
     4801 ccataggtgg cgacaaggat cggggtctgg gtcatcgctg gcatcatcga ctgtacgaag
     4861 aagacctgct tcatgagcat gggcaggtcg gttaggcgta gcaataggat gacgatagcc
     4921 ggcgagacga cgaagcggca gagggccacg agtaccaggt cgcgttcgag gcgtatcgag
     4981 cgccagtcga cctttgcgat gacgatgccg atgaacagca tcgaaagggg cgtggtcatc
     5041 gagcctatgg tcctggccgt gtcgaggagt ggcttgggta ggtcgacgcc gagcatgatg
     5101 aggacgatgg cgaccatgag gccgaggagc ggcggagaca gtagcctgcg gagccccgtc
     5161 ggagataaga accgcggggc tgggttcccg gccaggcggg ccccgtctcc tgagatgcca
     5221 tagacgccga gggtccagaa catgacggta ttggcgatat agtagatgag gacgtagggc
     5281 aggctctcct cgccaaagat catcatgtta acgggtaggc cgatgaagat ggtgttcgat
     5341 agcgacgcca tcgacgagaa ggtgccgcgg cgttccggtc gtatcttgaa tagggcggct
     5401 acggcggcac tcgacgcgaa ggcggccgcc atcgacagga aggggatcgg caagcccggt
     5461 agcatcgaca ggagcctggc tctgtcgtag ccgcccatca ggttcgctat catgtaggcg
     5521 ggcagggaca cgttgacgac gagccgcgat atcagcttgc cgccgaaatc ctcggcttgg
     5581 ccgttcgcgc cgcctccggc gtagccgtca ctatccttgg atgcacgttc gcgaacggct
     5641 tggccgttcg cgccgcctcc ggcgtagccg gaggcgctga accaagaccg cctggccagc
     5701 acgaagccga gggctatcat caagaggacc gagaacacgc taccgagggc gttgagcatg
     5761 gcgccatcct atcgggaagg gcgttccatg ctcaagcccg ggctatcgca ggactcggtt
     5821 gaacagcggc gtgccgtcct gatcctggaa ctcgcaggcg aggccgtcgg gcgtcagttc
     5881 gagcacgagc cttccgaaga cgccctggtt ccgccagacc gagcctggcg agacgtagct
     5941 gggcacgggg tcgggcttgc cgccaagcgc gccgactacg gcccacgcgg tgccgtcggc
     6001 ttcaatccat tccatatagt gattgtgccc ggagatgacg aggtcggcct ttccggctat
     6061 gagcggcgct acctcgcgca gcatgtcggc gtgatcgtac caggccattc ccgtctcctc
     6121 atcgatatag ccggagctat agaagaagca gtgcgaaagt acgaccgtgt aatccgacgg
     6181 cgggatagcc gtcagctggg cgacgagcca ggccttctcc ttggcgccga agccttcagg
     6241 accccagagc aggtcgaggg ctatgaagtg gacgcgtccg gcgtcgattc gccatgacga
     6301 tgggctcggg ccggtaggcg ccgaagagcc gcctttcggc ccgaatgcgg cctgccagcg
     6361 ggcgtagccg cctatgagcg cgtcatggtt gccgagcagg gccaggaagg gcagggtcgg
     6421 ggcgtcgcgc cgtatcgact cgatggcctc ccgccaatcg tcagcctcca tgcccatctc
     6481 gacgatgtcg ccgaggttga taaaggcgtc gagcccgccc gagcgatgct ccacctcgga
     6541 tagccgtaga atagcgctcc tggcctcggc gttcgacgcg tcgcggccgt agtgcccatc
     6601 cgaagagacg gctaccctga ggatcggttc gccgtcggcg cccgtacgtc tagggatacc
     6661 gtcgctcggg gctatggtcg tcgggcgatc aggcgatgcc gcccggccgt acgcgttcgt
     6721 gaaaatgtac gcggatacgg agattgccgt caggagcgtg acggccgacg cggcgaagac
     6781 gatgccggtc gctatcctcg gcgccgcgcc cttggttcgg ctcagggccc tggcggccgc
     6841 cgccgcgacg ataggtagcg cgccgagcgc tatcaggccg agcaccggcg ctcggtaccc
     6901 ccatcggtac atgccgagcg tgccgggctg ggcgaatgcc cataccagga acgcagccat
     6961 ggccgcgcta gccgcaccgg cggctagcca gacctgttcg cctttcgtcg atttcatcgg
     7021 agcctccgcg cttagcctac gacgcactcg ccggacggtc aagcgatccg tagtgtcacg
     7081 aagcctattg gtttttaaac aaggcggccg ccccattcgg agcggccgcg gactcgttca
     7141 gagggccgaa gccctcctgg caaagtcgat catcgatcac ctcctttctc cggcgctcgc
     7201 gcgccaagat gttggtaatg ccggcatgat cactatacca cggcgccgcc gcccggaaaa
     7261 gcgacagtac gcgtctatcg gatctttcct ccccggcgag tggaatgatc gattatatcg
     7321 attcgatccg tttacacgta tgtgggcttg tgataggatg ctccgcatct tcgcgataca
     7381 tggaggttca ctatgataat gatccgaaac cgagcagtcg atagccccct gacccatggc
     7441 gccgccgagg cgtagcgaag aggaaagccc accatagctc gttcctcctc gcggtcattg
     7501 gcggatgtaa cgtacatcct tcgaggaacg atcatgccat atttgaatgc ggacggcgta
     7561 ttgcctcccg aattactgag cgaaatacag aagtacgtgc agggcgcctt ggtctatgta
     7621 cccaggcagg ggccggagcg tctcggatgg ggccagaaga acggcgccag ggacgagctg
     7681 gacaggcgca acgcggctat tcgagaggcg aaggcctgcg gacgtaccat cgatgagctg
     7741 gccgacgagt actgcctatc ggccgacggc attaggaaga tcctatactg taaccggcgc
     7801 gcttcatgag cgcgctcggg gcggcggggc tgcgacgacg atgtcgcgtc ccgccgcttt
     7861 tcctttatac atggcagcgt cggccctggc tatcagctcg tcgataggct cgcccttggc
     7921 gaactccgct acgcctatcg tcatcgatat atggagcctc tccgtcccca ccgtaacggc
     7981 ttcggccgac actgccttgc gtattcggtc gctggcctcg agcgcggcgg gcagcgagtc
     8041 acccgagagc aggatcagga attcctcgcc gccccagcgc gcagccatgt cttccattct
     8101 gacgctgcgc tcgacggcgc ccgcgacatg cttgagcacg aggtccccgg tctcgtggcc
     8161 gtgcaggtcg ttgaacgcct tgaagtggtc gatgtccccg atggccacgc agaacggcgc
     8221 ggaggaacgg ctcgaacgcg cgttctcgag cgcaagccgg ttggcccccg atcggcggtt
     8281 gcgtagtccg gtcagttcgt cgcgctcagc cagctccttc
//
LOCUS       DAOPHV010000042         8199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_11041_length_8199_cov_4.110634, whole genome shotgun sequence.
ACCESSION   DAOPHV010000042 DAOPHV010000000
VERSION     DAOPHV010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8199
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11041_length_8199_cov_4.110634"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PLQ29_02005"
     CDS             <1..547
                     /locus_tag="PLQ29_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85445.1"
                     /translation="DYSYFIAPHMDTRLLYDSLYDLDSDQVMQASICLNSAIPDCSPP
                     GTAIVSITAGIRPEAWSCVEAKDYFRIKSLLADKLIGQFEAATGARLRGHIEELEVAT
                     PVTFARYTGAWDGVVYGYEPAPWDSILPRAISLDRERYLAGLDFCGGFSYRCHGYGSS
                     ILSGKAAAERTLARLGARGGR"
     gene            544..1755
                     /locus_tag="PLQ29_02010"
     CDS             544..1755
                     /locus_tag="PLQ29_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding domain-containing
                     protein"
                     /protein_id="HPG85446.1"
                     /translation="MSVTLRGPVRDVLAFVAPFRERSRRFRDAPGEPKAPGHVTELAK
                     ALHPAGLQLRILSVRDDSPTTRTYRLAQADGSACLPPFRAGQYLSVFVEAGEVRASRP
                     YSISSTPDEAEGGGYYELTVKNAPGGFCAPLILGTWEPGTTVRCSAPAGVFYHEPLRD
                     GERLVCLAGGCGVTPFRSIVGDALAHGRASLALIHGATDPTELLFSGYFGELAVRYPE
                     RFSYLPIVERDGPAGVWGGERGYITAALIERLAPDASDAAYFISGPDAMRRFLADELA
                     PWGLPSRRTRLEAAPARFGPDAWPGYPRDASSLAFTLEARIGPETVSVPAAANETLLV
                     AMERAGLRPPSLCRAGECGWCRSRLVEGRCYSPPGPGARAADERFGFVHPCKAFPLSN
                     TILEVPENPDR"
     gene            1770..2633
                     /locus_tag="PLQ29_02015"
     CDS             1770..2633
                     /locus_tag="PLQ29_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPG85447.1"
                     /translation="MVLIADSAVQLNPETLDELGVSVVEYPMFLNGEPYPVSMRMNRA
                     EKDELRRLLKDKDNKVTTSGLREEDLVALYDRHKGERILSLHQSSRASSATAAVVRKI
                     VSERKDLDVTYLDSWHLTGAYSALVLDTARAVRDGLSYEEVIARFERARAATRHLGVV
                     YDLFYLHRTGRIGLAKAVLGSAMKIIAILSSSEEPGTLKSIGKVKNHVQANQRFAAIV
                     KEDMEARGARRLSAVISVIGPHEEEADHLKRAVEGLGYEAAITVSYTNHSNMPHAGPD
                     FYDIGYIAHVD"
     gene            2623..4203
                     /locus_tag="PLQ29_02020"
     CDS             2623..4203
                     /locus_tag="PLQ29_02020"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HPG85448.1"
                     /translation="MSIEALDAEGLRGAFVKAGEYLSLNQAVLDNLNVFPVPDGDTGA
                     NMVATLQAGLRALEDRPASSLRELSESMRRALLRNSRGNSGFIVAWFFSGFLAAVERH
                     ERLTRAELLEGFENGAYQVNSSLFTPVEGTMITIIAAMTRALHECPALDLGGCLRHAL
                     AAARESLFLTPTMLPLLAKAGVVDAGALGFIFIVEGMLRGLTNASVTAEPEDGYRFKP
                     DPAALLDWKPQPEYRYCTEVYVEEPRDCDGGSLRDFLGNRGNSIALVVDPRFIKLHIH
                     TDDPDGVIGYLGALGRIGSSKVEDMREQAAAVVAAHLDDSACAVLACVPGPGFAELFA
                     GLGVERCLEYGKELPSAGAILDALTEIEAPAVIILPNNANIAPAATLARDLCGRDVVV
                     VPTRNVVEGVAAAYGYSENDGLGDNARNMGDCVGLAECLCLYRSAPTPPSGTWPSRRE
                     RTSSCSATRWRPWATTPSARLSRPSRRLAWRAKATSRRTWGGTSTRPCSTVSGRGSPR
                     STRTPSSSRCTAGRAANS"
     gene            4232..4906
                     /locus_tag="PLQ29_02025"
     CDS             4232..4906
                     /locus_tag="PLQ29_02025"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HPG85449.1"
                     /translation="MRYLVATLAVAVSYLSGSVLYAVVVTRLVTGKDIRKQGNLNPGT
                     SNVARTVGKGWGVLVCALDSLKAIVPMLAARLWLFKGDDGASLAFVYAVGMAAVVGHC
                     RPIFYGFKGGGGIGTMLGVSLFMIPVEFAFSLLAGGVVAITAFRHTEHKYAQWTPIMF
                     VILTPFVTLATTLLVDLPLFAHVSIGGHPWPVVACAFAMSLSLLWFNGSFMRNRAAEY
                     QGLKGE"
     gene            complement(4942..5235)
                     /locus_tag="PLQ29_02030"
     CDS             complement(4942..5235)
                     /locus_tag="PLQ29_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HPG85450.1"
                     /translation="MLTSKQRARLSSLANELNPIVFLGKAGPVDGVHEALDRALTDHE
                     LVKLRFVDFKGERKELARELAEKAGAELVRVIGHVAIIYRPSPDPQKRAIKLD"
     gene            complement(5248..5829)
                     /locus_tag="PLQ29_02035"
     CDS             complement(5248..5829)
                     /locus_tag="PLQ29_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HPG85451.1"
                     /translation="MLRTVLGLLSAATSIYMLLCVVRVFMSWLPSARLGKPAELVERA
                     TEPYLSWWRRFPILRSGGVDFSPIAALAALSGLARLFTVASYGGLSLGVALALVVEVV
                     WSPFGFLLGFFAVLVIARIVAYAARWNSLNPLWRAVDAMINPVLFRIKRFVYKDRIVN
                     YMQGLVTGAVALVGARVLLGMAFGLVIGLLQRF"
     gene            complement(5854..7125)
                     /locus_tag="PLQ29_02040"
     CDS             complement(5854..7125)
                     /locus_tag="PLQ29_02040"
                     /inference="COORDINATES: protein motif:HMM:NF015634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0164 family protein"
                     /protein_id="HPG85452.1"
                     /translation="MKRIVGLALLLAILPRAGAEAQNLYADAYEFIYSMFSLGGEDPN
                     EGMTTFLSTLVPVGGSAESMGMAFVAVADDASFLELNPAGSALQDRTQFAFYHNNWIA
                     DTRLEAVVYTMRLGSLGLSLGGKWLYLPFTEYSDFGERVATGYYSEALGTFNASLHLF
                     PGYYFYGVSLGASAKLAFRSMPGYSDDEGGFVSGLSQSALAVMADIGALTRFNLFKLY
                     NSREKNFSVGLALKNVGPAVRDEPLPTVATAGFSYKFLKPILLSADISKPINLVDPSL
                     SESFYWGVGYRMVVTDFWDLQAGFLIKGDNPRISVGAAIDFSPLTLEVNYTLDLTTQF
                     SPLNRMSIEARFDMGDMGRTARAARAEELYLQGLDAYAAGDLPLAISYWNESLRLNPN
                     FDPARENRDTAQEALNLRQRMLELQRLEQGD"
     gene            7281..>8199
                     /locus_tag="PLQ29_02045"
     CDS             7281..>8199
                     /locus_tag="PLQ29_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85453.1"
                     /translation="MKMSRPMALVMLILLCCSSTAWAMGGREDPLAYADTLIAQQKYD
                     EAILYITDFMKRYPDRFDEAQKKLRQITKIRAAYNKSAETLIDVMTNDPTNQEKKLAM
                     IRELEGYERSPNPAVQEFVAKTKDLALFTYNRAKFEEHMAQGRSLIDSGRYVEAARAY
                     EAGFTLYRQEFAEAGLDRGFVDDAFERVASVSGAIITFEEQGEAMKTAFEELAAAYSG
                     GEAASIDAAWLRARSSAASLAATRRFVVDQGRALERSFAELSSKDATATDNSFLPFAF
                     RLVLGRQSEGRLEGVAGAIDARWAAALGSA"
BASE COUNT         1378 a         2786 c         2712 g         1323 t
ORIGIN      
        1 cgactacagc tacttcatcg cgccgcacat ggacacgcgg ctcctgtacg actcgctgta
       61 cgacctggac tcggaccagg tgatgcaggc gtccatctgc ctcaactcgg ccatccccga
      121 ctgctcgccg ccgggcaccg ccatcgtgtc catcaccgcc ggcatcaggc ccgaggcttg
      181 gtcctgcgtc gaagccaagg actacttcag gatcaagtcg ctcctggccg acaagctgat
      241 aggccagttc gaagcggcca ccggcgcgcg gctgcgcggc catatcgagg agctcgaggt
      301 cgcgacgccg gtcaccttcg cgcgctacac cggcgcctgg gacggcgtcg tctacggcta
      361 cgagccggcg ccctgggact ccatcctgcc gcgcgccata tccctggacc gagagcgcta
      421 cctggccggc ctggacttct gcggcggctt ctcgtaccgc tgccacggct acggtagctc
      481 catcctctcg ggcaaggccg ccgccgagcg gaccctggcc aggctcggcg caaggggcgg
      541 acgatgagcg tgacgctacg cggccccgtt cgcgacgtcc tggccttcgt cgcgccgttc
      601 cgcgagcgct cgcggcgctt ccgcgacgcg cccggcgagc ccaaggctcc cggacacgtg
      661 acggagctgg ccaaggccct gcatccggcc gggctccagt tgcggatact gtccgtacgc
      721 gacgacagcc cgacgacgag gacctaccgc ctggcccagg ctgacggttc ggcgtgctta
      781 ccgccgttca gggccggcca gtacctgagc gtattcgtcg aggcgggcga ggtacgcgcc
      841 tcgcggccct actccatatc gtcgaccccg gacgaggcgg aaggcggagg ctactacgag
      901 ctgacggtca agaacgcgcc aggcggcttc tgcgcgccgc tcatcctcgg gacctgggag
      961 ccgggcacga ccgtccgctg ctccgctccg gccggcgtct tctaccatga gccgctacgc
     1021 gacggcgagc gcctcgtctg cctggccggc ggctgcggcg tcacgccgtt caggtccatc
     1081 gtcggcgacg ccctggccca tggcagggcg agcctcgcgt tgatccacgg cgcgaccgat
     1141 cctaccgagc tattgttctc aggctacttc ggcgaactgg ccgtgcgtta tccggagcgc
     1201 ttctcgtacc tgccgatcgt cgagcgggac ggtcccgccg gcgtctgggg cggggagcgc
     1261 ggctacatta ccgccgccct gatcgagcgc ctggcgcctg acgccagcga cgcggcgtac
     1321 ttcatcagcg gcccggacgc gatgcggcgc ttcctggccg acgagctggc gccatggggc
     1381 ctgccgtccc gtcgaaccag gctggaagcc gccccggcgc gcttcggccc ggacgcctgg
     1441 cccggctacc cacgggacgc ctcatcgctg gccttcacgc tcgaagcgcg catcggcccc
     1501 gagacggtct cggtcccggc cgccgcgaac gagaccctgc tcgtggcgat ggagcgcgcc
     1561 ggactgcgcc ctccgtcgct gtgccgcgcg ggggagtgcg gctggtgccg ctcgcgcctc
     1621 gtcgagggcc gctgctactc gccgccgggg ccgggcgcgc gggccgccga cgagcgcttc
     1681 ggtttcgtgc acccgtgcaa ggctttcccg ttgtcgaata ccatactaga ggttccggaa
     1741 aaccccgacc ggtagccagg aggcgagcga tggtactgat agccgattca gccgtgcagc
     1801 tgaatcccga aaccctcgac gagctgggcg tcagcgtcgt cgagtaccct atgttcctga
     1861 acggcgagcc ctaccccgtc tcgatgcgta tgaaccgggc ggagaaggac gagctcaggc
     1921 ggctgctcaa ggacaaggac aacaaggtta ccacctcggg cctcagggag gaggacctcg
     1981 tcgccctgta cgatcgccac aagggcgagc gcatcctctc gctccaccag tcgagccggg
     2041 cctcgtcggc caccgcggcc gtcgtcagga agatcgtctc cgagcgcaag gacctcgacg
     2101 tcacctacct cgactcctgg cacctgaccg gcgcgtacag cgccctcgtg ctggacaccg
     2161 cgcgcgccgt ccgcgacggc ctgtcgtacg aggaagtcat cgcccgcttc gagcgagcgc
     2221 gcgccgcgac gaggcacctc ggcgtcgtct acgacctgtt ctacctgcac cggaccggca
     2281 ggataggcct ggccaaggcg gtcctcggct cagcgatgaa gatcatcgcc atcctgagct
     2341 cttcggagga accgggcacg ctcaagtcga tcggcaaggt caagaaccac gtccaggcca
     2401 accagcgctt cgcggccatc gtcaaggaag acatggaggc gcgcggagcc aggcggctat
     2461 ccgccgtcat cagcgtgatc ggcccccacg aagaggaggc cgatcacctg aagcgagccg
     2521 tagaggggct cggctacgag gccgcgatca ccgtgtcgta caccaaccac agcaacatgc
     2581 ctcacgcggg cccggacttc tatgatatcg gatacatcgc tcatgtcgat tgaggccctc
     2641 gacgccgagg gtctgcgagg cgccttcgtc aaggccggcg agtatctcag cctgaaccag
     2701 gcggtactcg acaacctgaa cgtgttcccg gtaccggacg gcgataccgg cgccaacatg
     2761 gtcgccacgc tgcaggccgg cctccgcgcg cttgaggacc gaccggcctc gagcctccgc
     2821 gagctgtccg aatcgatgcg gcgcgcgctc ttacgcaaca gccggggaaa ctccggcttc
     2881 atcgtcgcct ggttcttctc cggcttcctg gcggcggtcg aacgccatga gcgcctgacc
     2941 cgggcggagc tgctcgaggg ctttgaaaac ggcgcctacc aggtcaactc gtcgctgttc
     3001 accccggtcg agggcacgat gatcaccatc atcgcggcga tgacccgggc cctgcacgag
     3061 tgccccgccc tcgacctagg aggctgtctg cgccacgcgc tggccgccgc tagagagtcg
     3121 ctgttcctga cgccgaccat gctgcccctg ctcgccaagg ccggagtggt cgacgccggc
     3181 gccctcggct tcatcttcat cgtcgagggc atgctgcgcg gcctgaccaa cgcctcggtg
     3241 accgccgagc cggaggatgg gtaccgcttc aagccggatc cggccgccct cctcgattgg
     3301 aagccccagc cggagtaccg ctactgtacc gaggtctacg tcgaggaacc gcgcgattgc
     3361 gacggcggct cgctccgtga cttcctcggc aaccgcggca acagcattgc gctcgtggtc
     3421 gatccgcgct tcatcaagct gcacatccac accgacgacc cggacggcgt catcggctac
     3481 ctgggcgcgc tcgggcgcat cggctcgtcc aaggtcgagg acatgcgcga gcaggcggcg
     3541 gccgtcgtag cggcgcacct cgacgactcg gcctgcgccg tgctcgcctg cgtcccgggg
     3601 cccggcttcg cggagctgtt cgccgggctc ggggtcgagc gctgcctcga gtacggcaag
     3661 gagctaccga gcgccggcgc cattctggac gcgctgaccg agatcgaagc gcccgccgtc
     3721 atcatcctgc cgaacaacgc caacatcgcg ccggcggcca ccttggccag ggacctctgc
     3781 gggcgggacg tggtcgtcgt gccgacgagg aacgtcgtcg agggcgtggc ggcggcctac
     3841 ggttactccg agaacgacgg tctcggcgac aacgcgcgga acatggggga ctgcgtcggc
     3901 ctggccgagt gcctatgcct gtaccgtagc gcgccgactc caccttcggg gacctggcca
     3961 tcccggaggg agcgtacttc gtcatgctcg gcgacgaggt ggcggccatg ggcgacgacc
     4021 ccgtcggcgc ggctatcgag gccctcgagg cggctggcct ggcgggcaaa agcaacgtct
     4081 cggcgtacct ggggcgggac ttcgacccgg ccctgctcga cggtctcagg gcggggatcg
     4141 ccgcgatcaa cccggacgcc gtcttcgagt cgctgtacgg cgggcagggc cgcgaactcc
     4201 tgattatatc gctcgagtaa ggatgggacc catgcgctac ctcgtcgcga cgctcgccgt
     4261 cgccgtcagc tacctatcgg gctcggtgct ctacgccgtc gtcgtgacga ggctggtgac
     4321 cggcaaggac atacggaagc agggcaacct gaaccccggc acctcgaacg tcgcgcggac
     4381 ggtcggtaag ggttggggcg tcctcgtctg cgcgctggac agcctcaagg ccatcgtccc
     4441 catgctcgcg gcgaggctgt ggctattcaa gggcgacgac ggggccagcc tcgcgttcgt
     4501 gtacgcggtc ggcatggcag cggtcgtcgg ccactgcagg ccaatcttct acggcttcaa
     4561 gggcggcggc ggcataggca cgatgctcgg cgtctcgctg ttcatgatac cggtcgagtt
     4621 cgcgttctcg ctactggccg gcggcgtcgt cgcgatcacg gcttttaggc ataccgagca
     4681 caagtacgcc cagtggacgc cgatcatgtt cgtcatcctg acgcccttcg tcaccctagc
     4741 tacgaccctg ctcgtcgacc tgccgctatt cgcgcacgtc tcgataggcg gccacccctg
     4801 gcccgtcgtc gcctgcgcct tcgcgatgtc gctgtcgctc ctgtggttca acggctcgtt
     4861 catgcggaac agggccgccg agtaccaggg cctcaagggc gagtgaacgg cgccgggccg
     4921 gagcgcggcc ccgccgcccg cctaatcgag cttgatcgct cgcttctgcg ggtccgggct
     4981 cggcctgtag atgatggcca catgcccgat cacgcgaacg agctcggccc ccgccttctc
     5041 ggccagctcc ctggccagct ccttgcgctc gcccttgaag tcgacgaaac gtagcttgac
     5101 cagctcgtgg tcggtcaggg cgcggtcgag cgcctcgtgg acgccgtcga ccgggccggc
     5161 cttgccgagg aatacgatgg ggttcagttc gttggcgagc gaggacagcc tggcacgttg
     5221 tttcgatgtc agcatgcgtt gttcctttta aaaccgctgc agaaggccta tgacgaggcc
     5281 gaaggccatc ccgagcagca ccctggcccc cacgagggct acggcaccgg tgacgaggcc
     5341 ctgcatatag ttgacgatcc ggtctttgta gacgaagcgt ttgatcctga acaggactgg
     5401 gttgatcatg gcgtcgacgg cccgccagag cggattgagg ctgttccatc tggcggcgta
     5461 ggcgacgatg cgcgctatga cgagcacggc gaagaagccg agcaggaagc cgaacggcga
     5521 ccagacgacc tcgacgacca gggccagggc cacgccgagg ctcagcccgc cgtacgaggc
     5581 gacggtgaac agcctggcca ggcccgacag cgcggcgagc gcggctatcg gcgagaaatc
     5641 gacgccaccg gagcggagta tcgggaagcg cctccaccag gacaggtagg gctcggtggc
     5701 gcgctccacg agttcggcgg gcttaccgag ccgggcgctc ggtagccatg acatgaacac
     5761 gcggacgacg cacagcagca tgtagatcga cgtggccgcg gatagcagcc caaggacagt
     5821 tctcagcacg atggctccat tcgttccata gtctcagtcg ccctgctcca gccgttgcag
     5881 ctcgagcatg cgctggcgga ggttcagggc ttcctgggcg gtatcgcgat tctccctggc
     5941 ggggtcgaag ttcgggttga gcctgagcga ctcgttccag tacgagatgg ccagcggcag
     6001 gtctccggcc gcgtacgcgt ccaggccctg caggtacagc tcctcggccc gggccgccct
     6061 ggccgtccgc cccatgtcgc ccatgtcgaa gcgcgcctcg atgctcatgc ggttgagcgg
     6121 cgagaactgg gtggtcagat cgagggtata gttgacctcg agggtcaacg gcgagaagtc
     6181 gatggccgcg ccgacgctga tcctcgggtt gtcgcccttg atcaggaagc cggcctgtag
     6241 gtcccagaaa tcggtcacga ccatccggta gccgacgccc cagtagaacg actcgctcag
     6301 ggagggatcg accaggttga tgggcttgct gatgtcggcc gacaggagta tgggcttcag
     6361 gaacttgtac gagaagccgg cggtagcgac ggtcggaaga ggctcgtccc gcaccgcggg
     6421 gccgacgttc ttgagcgcca ggccgacgga gaaattcttc tcgcgggagt tgtagagctt
     6481 gaacaggttg aacctggtca gcgcgccgat gtccgccatc acggccaggg ccgactggga
     6541 cagtccggag acgaagccgc cctcgtcgtc gctatagccg ggcatggacc tgaacgccaa
     6601 cttggcgctg gcgccgagcg agacgccgta gaagtagtag ccggggaaca ggtggagcga
     6661 ggcgttgaag gtaccgaggg cctcggagta gtagccggtg gccacccgct cgccgaagtc
     6721 ggagtactcg gtgaacggca ggtacagcca tttaccaccg agcgacaggc cgagcgagcc
     6781 gaggcgcatc gtatagacga cggcctcgag ccgcgtgtcg gctatccagt tgttgtggta
     6841 gaacgcgaac tgcgtgcgat cctgcagggc gctaccggcc gggttcagct cgaggaagga
     6901 cgcgtcatca gccacggcga cgaaggccat gcccatgctc tcggcgctac cgccgacggg
     6961 caccagcgtc gacaggaagg tggtcatgcc ctcgttcggg tcctccccgc cgagggagaa
     7021 catgctgtag atgaattcgt aggcgtcggc gtacaggttc tgggcttcgg ccccggctcg
     7081 cgggagtatc gctaggagta gcgcaaggcc gactatcctc ttcatggtac aatggaatat
     7141 acagtacgat agagataatt tctatccgta taaacgatag taacgctcag cttagccgcg
     7201 cgcgagccga caatgatact gaggagccgg gcctcggagc gcccggccag ctcgctggag
     7261 gagcctgtag gcgcctatat atgaaaatga gccgcccgat ggcgctggtc atgcttattc
     7321 tcctctgttg ctcctcgacc gcctgggcaa tgggcggccg cgaggatccc ttggcctacg
     7381 cggataccct gatagcccag caaaagtacg acgaggccat cctctacatc accgatttca
     7441 tgaaacgcta tccggaccgc ttcgacgagg cccagaagaa gctcaggcaa attaccaaga
     7501 tcagagcggc ctacaacaag tcggctgaga ccctgataga cgtgatgacc aacgacccga
     7561 ccaaccaaga aaagaaactc gcgatgatcc gcgagctcga gggctacgaa cgctcgccga
     7621 acccggcggt ccaggagttc gtggccaaga cgaaggacct ggccctgttc acctataacc
     7681 gagccaaatt cgaggagcac atggcccagg gccgctcgct gatcgactcg ggccgctatg
     7741 tcgaggcggc cagggcctac gaggccggtt ttaccttgta tcgacaggaa ttcgccgagg
     7801 ccggcctgga ccggggcttc gtggacgacg ccttcgagag agtagcctcg gtctcgggcg
     7861 ccataatcac cttcgaggag cagggcgagg cgatgaaaac ggccttcgag gagctcgccg
     7921 ccgcatactc gggcggcgag gccgcgtcga tagacgcggc ttggctccgg gcccgttcgt
     7981 ccgcggcctc cctggccgcg acccgacgct tcgtcgtcga ccagggacgc gccctcgagc
     8041 gctccttcgc cgaactatcg tccaaggacg cgaccgccac cgacaactcc ttcctcccgt
     8101 tcgccttccg cctcgtgctc ggccgccagt ccgaggggcg gctcgagggc gtcgccggcg
     8161 cgatcgacgc ccggtgggcc gccgctctcg gctcggccc
//
LOCUS       DAOPHV010000043         8135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_11176_length_8135_cov_4.039356, whole genome shotgun sequence.
ACCESSION   DAOPHV010000043 DAOPHV010000000
VERSION     DAOPHV010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11176_length_8135_cov_4.039356"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..860
                     /gene="mazG"
                     /locus_tag="PLQ29_02050"
     CDS             33..860
                     /gene="mazG"
                     /locus_tag="PLQ29_02050"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPG85454.1"
                     /translation="MTRTATPEGAFKRLYDIFTRLRAPDGCPWDREQTPESIRGNLIE
                     EAYECVEAINDGEPNHVKEELGDVMLVATMMAVIHEERGDFTVAEALENVSDKLVRRH
                     PHVFADAEAETPDAVLRQWNDIKENVEGRRKKDSVLDSVSKALPPLERAYKLQKAAAK
                     VGFDWPDPTGPWDKLEEELRESREACAAIEAKAASGGDDSLERAALEDELGDLFFSLV
                     NVARKHRVDPAIAMHRALEKFSRRFRHVEKAMAAAGETMEPGKLALMDRYWDEAKRA"
     gene            complement(867..1625)
                     /locus_tag="PLQ29_02055"
     CDS             complement(867..1625)
                     /locus_tag="PLQ29_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85455.1"
                     /translation="MIGADIATVLETASMAGVRGVEWSDDGFFDPRDPSSVRAAMMAT
                     LRAGLCTVSYATLYRAVAHDRSAFRSALGVARDFNAPMLRLWSGPRGYGGGLAARAFL
                     DESRYLGDEAGKHGITLCFGISRRSVLASYDEATRVLAGTDHPFVKLAWEPASRGGFD
                     EAMEEFRPLSGRIGLIVVRGEDLLPEGGSADALSEEWYQYLDSYDEQCGSPDMARYVV
                     LSSTAGDQGRLAEAAGAIAAWSVMLRRYHRRRVM"
     gene            1793..3793
                     /locus_tag="PLQ29_02060"
     CDS             1793..3793
                     /locus_tag="PLQ29_02060"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF007746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HPG85456.1"
                     /translation="MIQLDAVDDGFVISLNGRRALLHSARVPLLELGTAETSARQRGG
                     AYKLGERRLSVVKAKAWKLATARDDFIDVEFDGLARMAIREAGGALRISLSRYDRAFN
                     RFMMRLPATPGESVFGCGERSSRLDLKGSIAPLWSQERGVAKGRGLAPLLSKARAGGA
                     WHATSLGQPSFVTSERSYVHVGTNARCVFDFRRPRLTALRCWAIPDELVIGFADDMPA
                     AVGALSAITGRQPALPPWTWDGVWLGVEGGMAELRRKLDATRRAGIPVGAVWVRDWSG
                     RVRAGSSSRPRWDWRYDRELYPDLPAEIAALASDGIRFIGYANPYLDVDGELYAEASR
                     KGFCVKRDDGSDYQLAAQARPAAMVDLFNPGAFSWIKSVLIRELLGIGMAGWMADAGE
                     HLPVDAALHGDRDPLLAHNEYPALWARACAEAIVEAGKGAEATFFCRSGWAGSSRHAG
                     AFWAGDRTAGWSRRGGLPSVVPASLSLGFSGAGIWHSDIGGDATTAWARRSDECLMRW
                     AETAAFTPIMRTHEGDRPETNAQPWSNPATLAHFARLSSVWSALAPYHAETLAEYASS
                     GLPPIRHSWLHYEDQPELRVLANQYLYGRDLLVAPVLEPGAELSKAVLPSDHWVHLWT
                     SREFMGGEVTIEAPLGYPPVFYRAASPWASLFESLRRTARKL"
     gene            complement(3794..4273)
                     /gene="ispF"
                     /locus_tag="PLQ29_02065"
     CDS             complement(3794..4273)
                     /gene="ispF"
                     /locus_tag="PLQ29_02065"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPG85457.1"
                     /translation="MRVGQGWDLHRLGSGRPLMLGGVEVPSELGELGHSDGDALFHAI
                     VDAMLGAVAAGDIGRHFPPSDPRWKDAPSRLFVERAAEILAGSGWRVVNVDATVVLER
                     PRLAPLADDIRASVADALGLSVGAVSVKAKTREGLGDVGRSEAIEAQAIVLVERLEP"
     gene            complement(4276..4941)
                     /gene="ispD"
                     /locus_tag="PLQ29_02070"
     CDS             complement(4276..4941)
                     /gene="ispD"
                     /locus_tag="PLQ29_02070"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPG85458.1"
                     /translation="MRFAAVIAAAGRSVRFGGAKKEYELLDGLPVLAHSVRLFIEMPD
                     CAAAAVVVPPGGEAEARAALGPALITRAGPRLLFVEGGAERADSVRAGLLALRGADPE
                     YVLIHDAARPWASAELVRRVLAGAARSGACAPGVPVTDTIKRVAADGTIAEHLERAAL
                     RAVQTPQGFLYRGLLGAYLSVGASSRAATDDAEIWAMAGGSVSVVDGDRANVKITFPE
                     DLR"
     gene            complement(4943..5443)
                     /locus_tag="PLQ29_02075"
     CDS             complement(4943..5443)
                     /locus_tag="PLQ29_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPG85459.1"
                     /translation="MSVSKTTEFGVDQRIVYPSQGVGRIVEIQEKRFKETSVLYYIIY
                     LEFSDMTVMVPVDKARDLGIRPIVPTAEAERALEFIAEEYAPIPSDWKLRYQMNLDLL
                     KKGSVMDIASVVRSLFHRSKIKELPILERKLYDSALNLLQDEVSFSLGKPKDDVAEMI
                     RAKLEG"
     gene            complement(5652..6449)
                     /locus_tag="PLQ29_02080"
     CDS             complement(5652..6449)
                     /locus_tag="PLQ29_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPG85460.1"
                     /translation="MIDPTDPSSAFAGLRDRDYARAGLMVCEGRIVVQKALDCGVRLR
                     SMLCVPADEEEWLGVSGGRFPVAALSRADMADLLGFQFHRGLLALADRPTSPEPAAAP
                     GGPALALWNVTDPDNLGALIRSAAALGAARVYLGPGCADPYSRKALRASMACALGLPV
                     LPLADASELELARGPSARGAVGGAVGGNLLVAAALVPVALTPDGARAAAAGRPVTLIL
                     GNEGWGLPSEVTAACDERVIVPMAGGVDSLNVGAAGAILMWELFRHP"
     gene            6610..8001
                     /locus_tag="PLQ29_02085"
     CDS             6610..8001
                     /locus_tag="PLQ29_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPG85461.1"
                     /translation="MRYENIGRHASRFKEYVELRIQENRSIGISLLNGDVVGNARGAQ
                     TGLSARAFKNGVWGFASSPELDDGAIRAAVDRAVENARFLGAREAAGALTKGPLPSRP
                     GRGDYGLGTAEPRKSQKDLIEFVRSIDAYCQKYYPGLSSRSVSLSCLDMEKTILTADG
                     AEAYSMTPRTIIAVSLTVEEGGEPIDLYEVYGGRGQFEDVFEDPRDLYPNIDALADNL
                     ARKSKGVYARPGVHDVVLDSDLAGILAHEAVGHTVEADGVLGGSVAADAMGRRVASPL
                     VTLVDFANTALGETCPVPIWVDDEGVEARDAVLIEEGVLRSYMHNKESAAHFGAEPTG
                     NARAYGFYDEPLIRMRNTAILPGESKLQDMIASVDDGYYFLRSSNGQADSTSEFMFGV
                     VLGFEIKGGKLGEAVRDTTISGVAFDMLKTVAAVSDDMSWSCGGMCGKKQSIPVGMGG
                     PAVRCRVNVGGRA"
BASE COUNT         1372 a         2857 c         2747 g         1159 t
ORIGIN      
        1 gccggcctcg ccacaatgcc ggcatggaaa cgatgacgag aaccgctacc cccgaaggcg
       61 ccttcaagcg tctctacgat atattcacgc gcctacgcgc ccctgatggg tgcccctggg
      121 accgggaaca gactcccgag agcatccggg gcaacctcat cgaggaagcc tacgagtgcg
      181 tcgaagccat caacgacgga gagccgaacc acgtcaagga ggagctcggc gacgtgatgc
      241 tcgtcgcgac gatgatggcg gtaatccacg aggagcgggg cgactttacc gtcgccgagg
      301 ccctcgaaaa cgtttcggac aagctggtcc gccgccaccc gcacgtgttc gccgacgccg
      361 aggccgagac cccggacgcc gtgctcaggc agtggaacga catcaaagag aacgtcgagg
      421 gacgccggaa gaaggactcg gtcctcgact cggtgtccaa ggccctgccc ccgcttgagc
      481 gggcctacaa gctgcagaag gcggcagcca aggtcggctt cgactggccg gacccgacgg
      541 gcccctggga caagctcgag gaggagctgc gcgagtcgcg cgaggcctgc gccgcgatcg
      601 aggccaaggc cgcttccggc ggagacgact cccttgagcg ggccgcgctc gaggacgagc
      661 tcggcgacct gttcttctcg ctggtcaacg tcgcgcgcaa gcaccgcgtc gaccccgcga
      721 tagcgatgca ccgggccctg gagaaattct cgagacgttt ccgccacgtg gagaaggcga
      781 tggcggcggc cggcgagacg atggagccgg gcaagttggc gctcatggac cgctactggg
      841 atgaggcgaa gcgggcctga tagcggctac atcacgcgac ggcggtgata gcgcctgagc
      901 atgacgctcc aggcggcgat cgcgccggcg gcctcggcca ggcgtccctg gtcgcccgcc
      961 gtcgagctga gcacgacgta gcgcgccatg tccgggctgc cgcactgctc gtcgtacgag
     1021 tcgaggtact ggtaccattc ctccgacagc gcgtcggccg agccgccctc gggcaggaga
     1081 tcctcgccgc ggacgacgat gaggccgatt cgtccggata gcggcctaaa ctcctccatc
     1141 gcctcgtcga agccgccgcg ggacgcgggc tcccaggcca gcttgacgaa gggatggtcg
     1201 gttccggcca ggaccctcgt cgcctcgtcg tacgaggcga gtaccgatcg cctcgatata
     1261 ccgaagcata gggtaatccc gtgcttgccc gcctcgtcgc ccaggtacct cgactcgtca
     1321 aggaaggcgc gcgcggcgag cccgccgccg tagccgcggg gccccgacca gaggcgcagc
     1381 atgggggcgt tgaaatcgcg ggcgacgcca agtgcggagc ggaaggccga gcgatcatgg
     1441 gcgacggcgc ggtacagggt ggcgtacgat accgtacaga ggccggcccg caacgtggcc
     1501 atcatcgcgg cgcgcaccga cgacgggtcg cgcgggtcga aaaagccgtc gtcggaccat
     1561 tcgacgcccc gtacgcccgc catggaggcc gtctcgagta cggtggctat atcggccccg
     1621 atcatcgccg aggaagagag cgccgcatgg atcatcctaa gtccggattc tacctgaagc
     1681 catggctgat ggcaaccttg cgccgccaga atcggtcttg cgggcccgat cgacccggta
     1741 tatggtaccg tatacggtcg gccgagccgg ccgtctggcg ggaggtcttc cattgataca
     1801 gctcgacgcc gtcgacgacg ggttcgtcat aagcctgaac ggccgccgcg ccctgctcca
     1861 ctcggcccgg gtgcccctac tcgaactcgg cacggcggag acctcggcca ggcaacgcgg
     1921 cggcgcctat aagctcggcg agcgccgcct atccgtcgtc aaggccaagg catggaagct
     1981 cgccaccgcc cgcgacgact tcatagacgt cgaattcgat ggcctcgcgc gtatggccat
     2041 ccgtgaggcc gggggcgcgc tcaggataag cctctcccgt tacgaccgcg cctttaaccg
     2101 cttcatgatg cgcctcccgg cgacgccggg cgagagcgtc ttcggctgcg gagagcgatc
     2161 ctcgcggctg gacctcaaag gctcgatcgc accgctatgg tcccaggaac gcggcgtggc
     2221 caagggacga ggcctcgcgc cgctcctgtc gaaggcccgg gccggcggcg cctggcacgc
     2281 cacgagcctc ggccagccgt ccttcgtcac cagcgagcgc tcctatgtcc acgtcggcac
     2341 gaacgcccgc tgcgtcttcg atttcaggcg gccgaggctc accgcgctac gttgctgggc
     2401 aatccccgac gagctggtca taggcttcgc cgacgatatg ccggccgccg tcggcgccct
     2461 atcggcgatc acgggccgcc agccagcgct cccgccgtgg acctgggacg gcgtctggct
     2521 cggagtcgag ggcgggatgg ccgagttgcg gcgaaagctc gatgcgacgc gacgcgccgg
     2581 tattccggtc ggcgccgtct gggtccgaga ctggagcggc cgggtccgcg ccggctccag
     2641 ctcgcggccc cgctgggact ggcgctacga tcgcgagctg tatcccgacc tgccggccga
     2701 gatagccgca ctggccagcg acgggatccg tttcatcggg tacgcgaacc catacctgga
     2761 cgtcgacggc gagctgtacg ccgaggcgtc caggaaaggc ttctgcgtca agcgcgacga
     2821 cggctcggac taccagctcg ccgcccaggc tcgcccggcc gcgatggtcg acctattcaa
     2881 cccgggggcc ttctcgtgga tcaagtcggt actgatccgg gagctgctcg gcatcggcat
     2941 ggccggttgg atggccgacg cgggcgaaca tctgccggtg gacgcggcgc tccacggcga
     3001 ccgcgacccc ctgctcgccc ataacgaata cccggccctc tgggccagag cctgcgccga
     3061 ggcgatagtc gaggcgggca agggggcaga ggccaccttc ttctgccgct caggatgggc
     3121 cggatcatcg cgccacgccg gcgccttctg ggccggcgac cggaccgccg gctggagccg
     3181 ccgcggcggc ctgccgagcg tcgtgccggc gtcgctatcg ctcggcttct ccggtgccgg
     3241 catctggcac tccgacatag gcggcgacgc gacgacggcc tgggcgaggc gctcggacga
     3301 atgcctcatg cgatgggcgg agacggccgc gttcacgccg atcatgcgga cgcacgaggg
     3361 cgaccgaccc gaaacgaacg cccagccttg gtcgaacccc gccacgctcg cacatttcgc
     3421 gcgcctttcc tcggtctggt ccgcgctggc cccctaccac gcagagacgc tggccgaata
     3481 cgcctcgtcg ggcttgccgc ccatccgcca cagctggctg cactacgagg atcagccgga
     3541 gctgcgcgtc ctggccaacc agtacctcta cggccgcgac ctgctcgtcg cgccggtact
     3601 ggaaccgggc gccgagttat ccaaggccgt cttgccgtcc gaccattggg tgcatctctg
     3661 gacgtccagg gaattcatgg gcggcgaggt gacgatagag gcgccgctcg gctatccgcc
     3721 ggtgttctac cgggcggcgt cgccctgggc cagtctattc gagagcctgc gaagaaccgc
     3781 gcggaaactg tagctagggt tccagccgct cgaccaggac gatggcctgg gcctcgatag
     3841 cctcggagcg gccgacgtcg ccgagcccct cgcgggtctt ggccttgacc gacacggccc
     3901 cgaccgacag gccgagcgcg tcggccacgg aggcacgtat atcgtcggcc aggggagcga
     3961 gccgcggccg ctcgagcacc accgtcgcgt cgacgttgac gacgcgccag ccggagccgg
     4021 cgaggatctc ggccgccctc tccacgaaca ggcgggaggg cgcgtccttc cagcgagggt
     4081 ccgacggggg aaaatgccgg ccgatgtcgc ccgcggctac ggcgccgagc atcgcgtcga
     4141 cgatggcgtg gaacagcgcg tcgccgtcgg agtgcccgag ctcgcctagc tccgacggaa
     4201 cctcgacgcc gccgagcatc agcggcctgc ccgagccgag ccggtgcagg tcccagccct
     4261 gccccacccg caggctcacc ggaggtcctc cgggaaggtg atcttcacgt tcgctcgatc
     4321 gccatcgacg accgagacgg agccgccggc catggcccag atctccgcgt cgtcggtggc
     4381 cgcccgcgag gaggccccga ccgacaggta ggcgcccagg aggccgcggt agaggaagcc
     4441 ttgcggcgtc tggacggcac ggagggccgc acgctcgagg tgttcggcga tcgtgccgtc
     4501 ggcggccacg cgcttgatgg tatcggtcac cggcaccccc ggagcgcagg cgcccgagcg
     4561 cgccgctccg gcgagcacgc ggcgaacgag ctcggcgctc gcccatggcc tcgcggcgtc
     4621 gtggatcagc acgtactccg gatcggcgcc gcgcagggcc agaagcccgg cgcgcacgct
     4681 atcggcgcgt tcagcgccgc cttcgacgaa cagcagccgc ggcccggcgc gcgtgatcag
     4741 cgccggcccc agggccgccc tcgcctcggc ctccccgccc gggggtacca cgacggccgc
     4801 ggcggcgcag tcgggcatct cgatgaataa ccgtacggag tgggccagca ccgggagacc
     4861 gtcgaggagc tcgtactcct tcttggcgcc gccgaagcgg acgctcctcc cggcggccgc
     4921 gatgaccgcc gcgaaccgca cgctaacctt cgagcttggc gcggatcatc tccgcgacgt
     4981 cgtctttcgg cttgccgagc gagaacgaga cctcatcctg caggaggttg agggcgctat
     5041 cgtagagctt acgctcgagg atgggtagct cctttatctt ggagcgatgg aagagcgaac
     5101 gcaccacgct ggcgatgtcc atcacggagc ccttcttgag caggtccagg ttcatctggt
     5161 accggagctt ccagtcggac ggtatgggcg catactcctc ggcgatgaac tcgagcgctc
     5221 gctccgcctc cgcggtcggc acgatgggac ggatgcccaa gtcgcgcgct ttgtccaccg
     5281 gtaccatgac ggtcatgtcg gagaactcga gatagatgat atagtagagg acgctcgttt
     5341 ccttgaagcg cttttcctgg atctccacga tcctgccgac gccctggcta ggatagacga
     5401 tacgctgatc tacgccgaat tcagtcgttt tggacacact catacagact ccgattctac
     5461 tcctccgccg cctaaaagtc aaacacgccc atacgcgctc ccatacgcgc tcctatacgc
     5521 gcgccaggga tggcgcgcca caacccggcc cgtccgcagg acgggaacgt cacggatgac
     5581 gatgccgcgc acggatgcgc ggccacaacc cggcccgtcc gcaggacggg aacgtcatgg
     5641 atgacgaaac gtcatggatg acgaaatagc tcccacatga ggatcgcgcc ggccgcgccg
     5701 acgttgagcg aatcgacacc ccccgccatc ggcacgatga cgcgttcgtc acaggccgcc
     5761 gttacctccg agggcaaccc ccagccctcg tttccgagga tcagcgtcac ggggcgaccg
     5821 gcggccgccg ccctcgcccc atccggcgtc agcgccacgg gtaccagggc cgccgcgacg
     5881 agcaggttcc cgcccacggc ccctccaaca gctccgcgag cggacggccc cctggccagc
     5941 tcgagctccg aggcgtcggc tagcggcagt accggcagcc cgagcgcgca ggccatcgag
     6001 gcccggagcg ccttacggct atacgggtcg gcgcagccgg ggccgaggta gacgcgggcc
     6061 gctccgagcg ccgccgcgct ccgtatcaac gctcccaggt tgtcggggtc tgtcacgttc
     6121 cagagcgcga gggccggccc gccaggcgcg gcggcaggct cgggcgacgt cggcctgtcg
     6181 gctagcgcga gcaagccgcg gtgaaactgg aagccgagca agtcagccat gtcggcacgg
     6241 ctcagcgcgg ccacagggaa gcgaccgccc gacacgccga gccactcctc ctcgtcggcc
     6301 ggcacgcaga gcatcgagcg cagccgtacg ccgcagtcga gggccttctg gacgacgata
     6361 cgtccttcgc agaccatcag cccggcgcgg gcgtagtccc ggtcgcgcag gccggcgaag
     6421 gccgaggacg ggtcggtagg atcgatcatc gccggagtat accaaaatcg agacgctagg
     6481 gcgacaggcc tgccgcattg gccgaagccg acccttgccc tcgccgggct atgcaagtat
     6541 ggttagtatc ctacgccctt cgcgggcgcg ccccctcggg gcgtttcgat cacctgcccg
     6601 ggaggccgca tgagatacga gaacataggg cgccacgcgt ccaggttcaa ggaatacgtc
     6661 gagctacgca tccaggagaa ccgctcgata ggcatcagcc tgctcaacgg agacgtcgta
     6721 ggaaacgcgc gcggcgccca gacggggctg tcggcccgcg ccttcaagaa cggcgtctgg
     6781 ggcttcgcct cgtcgcccga gctcgacgac ggcgcgatcc gagcggccgt cgaccgggcg
     6841 gtcgagaacg cccgcttcct cggcgccagg gaggcggccg gcgccctgac gaagggcccg
     6901 ctaccgtcca ggccgggtcg cggcgactac ggcctcggca ccgccgagcc ccgcaagagc
     6961 cagaaggacc tgatcgagtt cgtccgctcc atcgacgcgt actgccagaa gtactatccc
     7021 gggctatcgt cgcgctcggt cagcctgtcc tgtctcgaca tggagaagac catcctgacc
     7081 gccgacggcg ccgaggccta ttcgatgacg ccccggacga tcatcgcggt atcgctgacc
     7141 gtcgaggagg gaggcgagcc gatcgaccta tacgaggtct acggcggccg cggccagttc
     7201 gaggacgtgt tcgaggatcc gcgcgacctg tacccgaaca tcgacgcgct ggccgacaac
     7261 ctggcccgca agtccaaggg cgtctacgcc cgacccggcg tccacgacgt agtgctcgac
     7321 tccgacctag ccggcatcct ggcccacgag gccgtggggc acacggtcga ggccgacggc
     7381 gtgctcggcg ggtcggtcgc cgccgacgcc atgggccgcc gggtcgccag ccccctcgtc
     7441 accctggtcg acttcgccaa cacggcgctc ggcgagacct gtcccgtgcc gatctgggtc
     7501 gacgacgagg gcgtcgaggc gcgcgacgcc gtgctgatcg aggaaggcgt cctgcgctcc
     7561 tacatgcaca acaaagagtc ggccgcccac ttcggcgccg agccgaccgg caacgcccgc
     7621 gcctacggct tctacgacga acccctgatc cggatgcgca ataccgcgat cctgccaggc
     7681 gagtccaagc tccaggacat gatagcctcg gtcgacgacg gctactattt cctgcgctcg
     7741 tccaacggcc aggcggactc gacgagcgag ttcatgttcg gcgtcgtgct gggcttcgag
     7801 atcaagggcg gcaagctcgg cgaggccgtc cgcgacacga cgatctcggg cgtcgccttc
     7861 gacatgctca agaccgtagc ggccgtgtcc gacgacatga gctggtcctg cggcggcatg
     7921 tgcggcaaga agcaatcgat acccgtaggc atgggcggcc cggccgtccg atgccgcgtc
     7981 aacgtgggag ggcgggcgta atggcagaat tcaacggcat ggaactcgtc aggaaggccg
     8041 tccaggccat gagggacgag ggggccgaca aggcccaggc gtcgacgttc agctcgctct
     8101 gctcgccgcg ggccgcatga gcctgtaccg cacca
//
LOCUS       DAOPHV010000044         8093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_11257_length_8093_cov_4.145310, whole genome shotgun sequence.
ACCESSION   DAOPHV010000044 DAOPHV010000000
VERSION     DAOPHV010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11257_length_8093_cov_4.145310"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLQ29_02090"
     CDS             <1..724
                     /locus_tag="PLQ29_02090"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPG85462.1"
                     /translation="NPCTTVCPTESIQLKARKGSILDLPYFEGSDCKGCSACVAACPG
                     LAVSLVRRAGDGMAEVRIPFEFDAGRYAIGDRLPALDADGRFVEDAELVAKQFYKKRR
                     TWVLTLRARPENAARIIGVRVQDEAVTRPLPEPRYEYLPDQAIVCRCERVTVGEMVAF
                     IKANEVRDASQLKSIRVGMGACGSKTCSALLPQVFRKAGVDPREVTDGTLRPLYLEAP
                     LGELAGRVAGPVGADERNGGRA"
     gene            721..1887
                     /locus_tag="PLQ29_02095"
     CDS             721..1887
                     /locus_tag="PLQ29_02095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG85463.1"
                     /translation="MRTCDVLIIGAGSVGVPLALYCAERGLSTIVLEKNASWGRGQNR
                     AAIGGLRATHSDPAKIRLCQESIRIVSGLEAERGIDVEWRAGGYLFVAYDEQRERAFR
                     DLLVTQKAAGLDIDWIGPERVAELCPGIRRDGLLGGTFSPGDGYASPLMTNTAFHRLA
                     RAAGAEFRFGEGVVSMATTPDRVSGARRIASVRTDKDEYSAAVVVDAAGADAADIAGL
                     VDLEIPVHPDCHEAGVTEPCERFLEPMLVDIRPDDESGNYYFYQTVTGQIVFCITPKP
                     QIWGKDTDSTSSFLPLVSRRMIELYPRLRNLKVRRTWRGMYPMTPDGLPIVGYPRECP
                     NLLLAVGMCGQGFMLGPGLGRLLADAIADGPEAAADVFEELSLYRKFEGMELLK"
     gene            complement(2019..2504)
                     /locus_tag="PLQ29_02100"
     CDS             complement(2019..2504)
                     /locus_tag="PLQ29_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85464.1"
                     /translation="MGCLEYFLDVIDAEFFGKSWNGPSLMATLDRLSAAEAADDATWE
                     GYSAWSIALHCAKCKRVVAVDLGVRAPDWDYGEETWFPAPADPSPSAWERDRALLRVM
                     HDLAMGALRALPEERLAEAMPSWKRPWGDVIAWLATHDAFHGAQIRSMGLTAFKEKRH
                     E"
     gene            2683..6768
                     /gene="pgk"
                     /locus_tag="PLQ29_02105"
     CDS             2683..6768
                     /gene="pgk"
                     /locus_tag="PLQ29_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPG85465.1"
                     /translation="MIIDEQAKRPARLDTWEAVLEQLGRVRDAADEAEKIELLVELIV
                     NLRLLGQDQAIPDRRTRSGDMESIYADLERATRAMLDSAVSPSSRFIYYAREVVKKLP
                     IKQDAEDMIEQIRMAAEAFEEERDEDDPMRGFAGDLRKEVHRRLSPRIIHAYLEPYLA
                     LVERGDPEPMIRAGYVALPGRVDPSDERGSFLDLARDLKERLDYLWDYGVGDMAAVKR
                     SLEQSMPLFERCFLRAEVSGFLEMLTPERLAKRHVAADMLKRLASFKRSIKESFTESR
                     ISLYDMLLVDLSLGRLIFLLANDLTNNRFLEVTPRNGKEALGVLAELLNISTVKGLAI
                     GDADRNLLALEELRAQSTYDYVRAKRCLGSICDELQGYLQRDIIDRMSGPLNRALEAY
                     GVPTSRLSQIKTRFFNNFIRRTQVHVLSEFVEKVRSAIDAELERQSVSRQLYADYRLA
                     EGGAAPAREACVAATWRETHDSIRPTLGGKGNGLIDMARLGLAVPPAFILGYPIFAAC
                     DADGELDAGLGAIVDEQLAELERRCGRRLGDPERPLVVSVRSGAPVSMPGVMVTVLNV
                     GLVPEVRRAICRRSDCDFAEALYRRFLDNAAGALAAMPPRTDSDQRERAPAELKSAPT
                     DELEAYLRASLGDGFLSDPREQLMQSIRLVYASRKSSAVDAFSRTLATTIRADTAVTV
                     QRMVFGNLNDASMSGVVITRDPITGADRLFGDFKRRAQGEEVVMGSVDTESIEHVDEA
                     VAAELEACKRRLVDYYRQDLDLEFTVEDGELYLLQTRAAKLGAFAQLVADKEFLERGL
                     IRLEEFHSRLERLEMANASIALPRADFRFRRWNPPLAVGVPINGGVVSGTLVLSDDCL
                     KEAEARRESVVYLAHTTKPTDFTVMNGANAIVTVYPGRTSHAAITAMSINRTCIVGVE
                     SAELDLAARLVRFRLADGSGHVTVAEGERVTADGNTGAIYRGVAPISEFFLPVSVITE
                     AIRREEDPVEAARAVGALIDEKLSGLSRETSMRRSGLDSPAARGMGRVIVRVDANVAL
                     ADGAVADRRSILRFADTLRTLLDRGATPIVCSHLGDPGAITGSVRSREEQYRDYSLRP
                     IADVLSGILGERFTFHETSVATSGLLVTASDMLEGKVNLLENLRFATGEKDNDDAFAM
                     AIAELSDGWYVNDAFNVCHRRHASIVGVPRFCRNRLAGPQVVAELSVIGALLDEPERP
                     FVAVFRGTSQATDLGAQLGVMSAIINRVDAMLVTGPGVEAIRPRLEALGHQAPGRLVV
                     LPSEAELKEALDGAGTVLWSGASGLSDDGWSEPDASGDPVAAALGRAAARNASVVVCS
                     ATDRYGRGAPGSRLHLSSGPAAFLEFLERLSLPGVTALDPD"
     gene            complement(6765..8012)
                     /locus_tag="PLQ29_02110"
     CDS             complement(6765..8012)
                     /locus_tag="PLQ29_02110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG85466.1"
                     /translation="MSAVRRIVNEYIEAFKSFSPNARRFLMGSFLLNVGANQISLLFS
                     LYLQRLAYTEAGIGAVLATRALGSTIIALPASILAARFDPRRLLPVAAAMTAAAYVAQ
                     SLFVSGAAISSAVFMAGALSTVFQVSAGPFFMRNSGEKERMHLFSLNGALSMGTGLIG
                     SLLGGGLKDGLAGLGVDELTAYRVGLLVGAGFVAAAVWPFSRIAPSREPRTMRAPGLR
                     RLDGIDVPLWLKLIFPSFLVGMGAGLTIPYLNLYFKNAFGMSDAAIGAAVAAGQVATF
                     LGIVAGPIIAKRIGKPRAVFWTQVLSVPLILVLGWVRALPLALLAYLARQVLMNMSSP
                     IQDNFALELVPPERQSLLNAVKMLSWTGSWTVAARVSGELIYRGGFKTSFALTAGLYL
                     ASTICYRIFFISGKRVRDGGRGA"
BASE COUNT         1322 a         2878 c         2705 g         1188 t
ORIGIN      
        1 caacccctgc accacggtct gcccgaccga gtccatacag ctcaaggcgc gcaaaggctc
       61 gatcctggac ctgccgtact tcgagggctc ggactgcaag ggctgctcgg cctgcgtcgc
      121 ggcctgcccg ggcctcgcgg tgtcgctcgt ccgccgggcg ggagacggca tggccgaggt
      181 caggataccg ttcgagttcg acgccggccg ttacgcgata ggcgacaggc tgcccgccct
      241 cgacgccgat ggccgtttcg tcgaggacgc cgagctcgta gccaagcagt tctataagaa
      301 gcgccgcacc tgggtgctga cgctgcgggc ccggcccgag aacgccgcca ggatcatcgg
      361 cgtgcgcgtg caggacgagg ccgtcaccag gccgctcccc gagccgcgct acgagtacct
      421 gcccgaccag gctatcgtct gccgctgcga gcgcgtgacg gtcggagaga tggtcgcgtt
      481 catcaaggcc aacgaggtcc gcgacgcgag ccagctcaag agcattcgcg tgggcatggg
      541 cgcctgcggc tccaagacct gctcggcgct cctgccgcag gtcttccgca aggcgggcgt
      601 cgacccgcgc gaggtgaccg acggcacgct caggccgctg tacctggagg cgccgctcgg
      661 cgagctggct ggccgcgtcg ccggccccgt gggagccgat gaacgaaacg gagggcgggc
      721 atgagaacct gcgatgtact gatcataggc gccgggtcgg tcggcgtccc gctcgccctg
      781 tactgcgccg agcgcggcct atcgacgata gtcctggaga agaatgcctc ttggggccgc
      841 ggccagaacc gcgcggccat cggcggcctg cgcgccaccc actccgaccc ggccaagatc
      901 aggctatgcc aggaatcgat ccgcatcgtc tcggggctcg aggcggagcg cggcatagac
      961 gtcgagtggc gcgctggcgg ctacctattc gtcgcctacg acgaacagcg cgagcgcgcc
     1021 ttccgggacc tgctcgtcac gcagaaggcc gccggcctcg acatcgactg gattgggcct
     1081 gagcgcgtcg ccgagctctg cccgggcatc cggcgcgacg gcctcctcgg cggcaccttc
     1141 agcccgggcg acggctacgc ctcgcccctg atgacgaata ccgccttcca taggctggcc
     1201 agggcggccg gcgccgagtt ccgtttcggt gagggcgtcg tttcgatggc gaccacgccg
     1261 gaccgcgtct ccggagcccg ccggatagcc tcggtgcgca ccgacaagga cgagtactcg
     1321 gccgccgtcg tcgtcgacgc cgcgggcgcc gacgccgccg acatagccgg cctcgtcgac
     1381 ctcgagatac cggtgcaccc cgactgccac gaggccggcg tcaccgagcc ctgcgagcgc
     1441 ttcctcgagc cgatgctcgt cgacatccgt cccgacgacg aatcgggcaa ctactatttc
     1501 tatcagaccg tcaccggcca gatcgtcttc tgcatcaccc ccaagcccca gatctggggc
     1561 aaggacaccg actcgacctc gagcttcctg ccgctcgtct cgcgccgcat gatcgaactg
     1621 tacccgcgcc tacgcaacct caaggtcagg cgcacctggc gcggcatgta cccgatgacg
     1681 ccggacggcc tgcccatcgt cggctatccg cgcgagtgcc cgaacctgct cctggccgtg
     1741 ggtatgtgcg gccagggctt catgctcggc ccgggcctgg gcaggttgct cgccgacgcg
     1801 atagccgacg gccccgaggc ggccgcggac gtcttcgagg agctgtcgct gtacaggaag
     1861 ttcgagggga tggagctgct aaaataagcg ctggcggccg ggcgctgtca gaccggccgt
     1921 cagaccggcc gtcagcctag ccgtcagcct agccgtcaga ccggccgtca gaccggccgt
     1981 cagcctagcc gtcggcccga ccgtcatccg cccggccgct actcgtgccg cttctccttg
     2041 aacgcggtga ggcccatcga cctgatctgg gcgccgtgga aggcgtcgtg cgtggcgagc
     2101 caggctatca cgtcgcccca tggccgcttc cagctcggca tggcctcggc cagacgctcc
     2161 tcgggtaggg cgcggagcgc gcccatggcc aggtcatgca tcacgcggag tagcgcgcgg
     2221 tcccgctccc aagccgacgg gctcgggtcg gccggcgccg ggaaccaagt ctcctcgccg
     2281 tagtcccagt cgggtgccct gactccgagg tcgacggcga cgacgcgctt gcacttggcg
     2341 cagtgcaggg cgatactcca ggcgctgtag ccctcccagg ttgcgtcgtc cgctgcctcg
     2401 gcggccgaga ggcggtcgag cgtggccatc agcgacggcc cgttccacga cttgccgaag
     2461 aactccgcat cgatcacgtc caggaaatat tccaggcatc ccatggctct accccctccg
     2521 atcggcggtc catcggcgcc agccggtggc gctccgccag aggcaagtat cgccgaggcc
     2581 agccgcgctg taaagggact atcacccgag gcaagccgga gcgagggtcg agccgttgac
     2641 caggcgcttc ctcctaagcg acatcgggtt tagactagcg ctatgatcat cgacgaacaa
     2701 gcgaaacggc ccgccaggct cgacacctgg gaagccgtgc tcgagcagct cgggcgcgtc
     2761 cgcgacgccg ccgacgaggc ggaaaagata gagctcctcg tcgagctcat cgtcaacctg
     2821 cgcctgctcg gacaggacca ggccataccc gaccgccgca cccggtcggg cgacatggag
     2881 agcatctacg ccgaccttga gcgcgccacc cgggccatgc tcgactcggc cgtctcgccg
     2941 tcgtcgcgct tcatctacta cgcccgggag gtggtcaaga agctacctat caagcaggac
     3001 gcagaggaca tgatcgagca gatacgtatg gcggccgagg ccttcgagga agagcgcgat
     3061 gaggacgacc cgatgcgcgg cttcgccggg gacctgcgca aggaggtgca tcgcaggctg
     3121 tcgccgcgca tcatccacgc ctacctggag ccgtacctgg ccctcgtcga gcgcggcgac
     3181 cccgagccga tgatcagggc cggctacgta gccctgccgg gccgcgtcga cccgtctgac
     3241 gagcggggct ccttcctcga cctggccagg gacctcaagg aacggctcga ctacctatgg
     3301 gactacggcg tcggggacat ggcggccgtc aagcgctcgc tcgagcagag catgcccctt
     3361 ttcgagcgct gcttcctgcg ggccgaggtg tcgggcttcc tcgagatgct gacgcccgag
     3421 cggctggcca agcgccacgt ggcggcggac atgctcaagc gcctcgccag cttcaagcgg
     3481 tccatcaagg agtcgttcac cgagtcgagg atatcgctgt acgacatgct gctcgtcgac
     3541 ctgtcgctcg gtcgcctcat cttcctcctg gccaacgacc tgacgaacaa ccgcttcctc
     3601 gaggtgacgc cgaggaacgg caaggaggcg ctcggggtgc tcgccgagct actcaacata
     3661 tcgaccgtca aaggcctggc tatcggcgac gccgaccgca acctgctggc gctcgaggag
     3721 ctgcgcgcgc aatcgaccta cgactacgtg cgagccaagc gctgcctcgg ctcgatctgc
     3781 gacgagctac agggctacct gcagcgggat atcatagacc gcatgagcgg cccgctcaac
     3841 cgcgcgctcg aggcctacgg cgtaccgacc tcgcgcctgt cccagatcaa gacgcgcttc
     3901 ttcaataact tcatacggcg cacccaggtc cacgtgctgt cggaattcgt ggagaaggtg
     3961 cgctcggcca tcgacgccga gctggagcgg cagagcgtca gcaggcagct ctacgccgac
     4021 tatcgcctgg ccgaaggcgg agcggccccg gcccgcgagg cctgcgtggc cgcgacctgg
     4081 agggagacgc acgatagcat acgcccgacg ctcggcggca agggcaacgg cctgatcgac
     4141 atggccaggc tcggcttggc ggtgccgccg gccttcatac tcggctaccc tatcttcgcg
     4201 gcctgcgacg ccgatggcga gctcgacgcc gggctcgggg ccatcgtcga tgagcagctg
     4261 gccgagctgg agcggcgttg cggccggcgc ctgggcgacc ccgagcggcc tctcgtcgta
     4321 tcggtacgct ccggcgcgcc cgtgtcgatg cccggcgtca tggtaacggt gctcaacgtc
     4381 ggcttagtcc cggaggtcag gcgcgcgatc tgccgtcgct cggactgcga cttcgccgag
     4441 gccctatacc gacgcttcct ggataacgcc gccggggcgc tcgccgcgat gccaccccgg
     4501 accgactcag accaacggga gcgggctccg gccgagctta agtcggcgcc caccgacgag
     4561 ctcgaagcct acctgcgcgc ctcgctcggc gacggcttcc tgtccgatcc gcgcgagcag
     4621 ctgatgcagt cgatccgcct cgtctacgcc tcgcgcaagt cgagcgccgt ggacgcgttc
     4681 tcgaggacgc tcgcgacgac gatacgggcc gacacggccg tgaccgtcca gcgcatggtc
     4741 ttcggtaatc tgaacgacgc ctcgatgagc ggggtcgtca tcacgcgcga cccgatcacg
     4801 ggcgccgacc ggctgttcgg cgacttcaag cggcgggccc agggcgagga ggtcgtcatg
     4861 ggctccgtcg acaccgagtc gatagagcac gtagacgagg ccgtggcggc ggagctcgag
     4921 gcctgcaaac gccgcctcgt cgactactac aggcaggacc tcgacctgga gttcaccgtc
     4981 gaggacggcg aactatacct gctccagacc agggcggcca agctcggggc cttcgcccag
     5041 ctggtcgcgg acaaggagtt cctcgagcgc ggccttatac gcctcgagga attccactcc
     5101 aggctcgagc gcctcgagat ggccaacgcg tcgatcgccc tgccccgcgc cgatttccgc
     5161 ttcaggcgat ggaacccgcc cctggccgtc ggcgtaccga tcaacggcgg ggtcgtgtcg
     5221 gggaccctgg tcctgagcga cgattgcctt aaggaggccg aggccaggcg cgagagcgtc
     5281 gtgtacctgg cccatacgac gaaacccacc gacttcacgg tcatgaacgg cgccaacgcc
     5341 atcgttaccg tctacccggg ccgcacctcg cacgcggcga tcaccgccat gtcgatcaac
     5401 aggacctgca tcgtcggcgt cgagagcgcc gagctcgacc tcgccgcccg cctcgtacgc
     5461 ttccgcctgg cggacggctc cggccacgtc acggtggccg agggcgagcg ggtgacggcc
     5521 gacggcaaca ccggcgccat ctaccgcggc gtggctccga tatcggagtt cttcctgccc
     5581 gtatccgtca taaccgaggc gatacgacgc gaagaagacc cggtcgaggc ggcgcgcgcg
     5641 gtcggcgccc tgatcgacga gaagctgtcc ggcctgagcc gcgagacgag catgcggcgc
     5701 tccggcctcg actcgccggc cgcccggggc atgggccgcg tcatcgtgcg agtcgacgcg
     5761 aacgtggccc tggccgacgg cgcggtagcc gaccgccgta gcatcctgcg cttcgccgac
     5821 accctgcgga ccctgctcga ccgcggcgcc acaccgatcg tctgctcgca cctgggagac
     5881 cccggggcga taaccggctc ggtacgctcc cgggaggagc agtaccgcga ctatagcctg
     5941 cggccgatag ccgacgtcct ctcgggcatc ctcggcgagc gcttcacgtt ccacgagacg
     6001 agcgtggcca cctccggcct cctggtcacc gcttccgaca tgctggaggg caaggtcaac
     6061 ctgctcgaga acctgcgttt cgccacgggc gagaaggaca acgacgacgc cttcgccatg
     6121 gccatcgccg agctgtccga cggctggtac gtgaacgacg ccttcaacgt ctgccaccgc
     6181 cgccacgcga gcatcgtcgg cgtgccgcgc ttctgccgga accggctcgc cggtccccag
     6241 gtcgtcgccg agctgtcggt catcggcgcc ctgctcgacg agcccgagcg gcccttcgtc
     6301 gcggtgttca gggggacgag ccaggccacc gacctcggcg cccagctcgg cgtgatgtcg
     6361 gccatcatca accgggtcga cgcgatgctc gtgaccggcc cgggcgtcga ggctatacgg
     6421 ccgcggctcg aggccctcgg gcaccaggcg ccgggccgcc tcgtcgtcct gccgtcggag
     6481 gcagagctga aggaggcgct ggacggggcc ggcaccgtgc tgtggtcggg cgcgagcggc
     6541 ctgtcggacg acggctggtc cgagcccgac gcctcgggag acccggtcgc ggccgccctc
     6601 gggcgcgcgg cggccaggaa cgcgtcggtg gtcgtctgca gcgccaccga ccgttacggc
     6661 cgaggggcgc caggctcgag gctacacctg tcgagcgggc cggcggcctt cctcgagttc
     6721 ctcgagcggc tgtccctgcc gggcgtcacc gccctcgatc ccgactaggc gccgcgtccg
     6781 ccgtcccgga cgcgcttgcc cgagatgaag aagatccggt agcagatcgt cgaggcgaga
     6841 tagaggccgg cggtcagcgc gaaggaggtc ttgaagccgc cgcggtagat cagctcgcct
     6901 gagacgcgcg cggccaccgt ccagctgccc gtccaggaca gcatcttgac cgcgttgagg
     6961 aggctctgcc gctccggcgg caccagctcg agcgcgaagt tatcctggat gggcgacgac
     7021 atgttcatga gcacctgcct ggccaggtag gccagcagcg cgagcggcaa ggcgcgcacc
     7081 cagccgagca ccaggatcag cggcaccgac agcacctgcg tccagaacac cgcgcgcggc
     7141 ttgcctatcc gcttggcgat gataggaccg gcgacgatgc ccaggaaggt ggccacctgt
     7201 ccggctgcca cggcggcgcc tatcgccgcg tcgctcatgc cgaacgcgtt cttgaagtac
     7261 aggttgaggt acggtatggt cagtcccgcg cccatgccga cgaggaagct cgggaagatc
     7321 agcttgagcc ataacgggac gtcgattccg tcgaggcgcc gcaggccggg cgcgcgcatc
     7381 gtccgcggtt cgcgcgacgg agcgatacga gagaacggcc acaccgccgc cgccacgaag
     7441 cccgccccga cgagcagccc gacccggtac gccgtcagct cgtcgacacc gagccccgcg
     7501 agcccgtcct tgaggccgcc gccgaggagc gagcctatca gccccgtgcc catcgacagc
     7561 gcgccgttca ggctgaacag gtgcatccgc tccttctcgc ccgagttgcg catgaagaac
     7621 ggcccggcgc tcacctggaa caccgtcgac agggcgccgg ccatgaacac ggccgacgat
     7681 atggccgcgc cggagacgaa cagcgactgg gcgacatagg cggccgccgt catcgccgcg
     7741 gcgaccggta gaagccggcg cgggtcgaag cgcgccgcca ggatagacgc gggcagcgct
     7801 atgatcgtcg agccgagcgc cctcgtcgcc agcaccgccc ctatcccggc ctccgtgtag
     7861 gccaggcgct ggaggtacag gctgaagagc aggctgatct ggttggcccc gacgttcagg
     7921 aggaagctcc ccatcaggaa gcgcctcgcg ttggggctga acgacttgaa ggcttctata
     7981 tactcgttga cgattcgtct gacagcgctc accccgcgag cttagccgcg ccggagcccg
     8041 tggtcaatcg gagctggcgt ttctacctag gctcccagga aagatgatat aat
//
LOCUS       DAOPHV010000045         8075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_11292_length_8075_cov_4.535661, whole genome shotgun sequence.
ACCESSION   DAOPHV010000045 DAOPHV010000000
VERSION     DAOPHV010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 8075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8075
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11292_length_8075_cov_4.535661"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PLQ29_02115"
     CDS             complement(<1..456)
                     /locus_tag="PLQ29_02115"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPG85467.1"
                     /translation="MSKKTIVVALGGNALIEEGTEGTIEQQFENTRKSMAAIVGLVKE
                     GHRVVLTHGNGPQAGVHLIRNEAGAAQVAPSSLNVIVADTQGSIGYMIQQSLMNALLK
                     AGVKTEVVTVVTQVVVDKDDPSMINPTKYVGPFFKAEHLDACKTKGWVMK"
     gene            complement(641..1480)
                     /locus_tag="PLQ29_02120"
     CDS             complement(641..1480)
                     /locus_tag="PLQ29_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85468.1"
                     /translation="MRTGLRIVRRAVPTGLVSALCLALLASCFSLPGIGGFNPLADLQ
                     SSVEARASAEVSSAVGLSGLERKVMFNVLYSQIFFIGGFGADFYPLEETQGAVWRIKS
                     VDEDGSFTAVEAERAFLKRLPNGDSWWYLAWRGDGESWEFEALMDKDQMARTIRYYNA
                     DVKRVEAAVFDTSGKAKPGSETAPPEEAPTSELALEDLSGYKKGVETIKVGAGTFRAE
                     RIEWTYYDEDEKTTYRYTWWVDPKASGGLVKYEWAKSGSKESVSGELVSLKKGYKTKF
                     ASF"
     gene            1674..2375
                     /gene="deoD"
                     /locus_tag="PLQ29_02125"
     CDS             1674..2375
                     /gene="deoD"
                     /locus_tag="PLQ29_02125"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPG85469.1"
                     /translation="MATPHNAAKKGDIAPTVLLPGDPLRAKFIAETFLENPVCYNTVR
                     NMFGFTGSYKGRPVSVQGTGMGVPSLSIYVTELIREYGAENVIRIGTCGSIQPDVKVR
                     DVIMAQGSCTDSNVNRIRFGGLDYAPIADFGLMRKAWKAAEDRGVALKVGNILTSDTF
                     YGDDPDSWKLWAAYNVMAVEMETAALYTIAAKFRKRALTLLTVSDSIVTGEATSAEER
                     QSTFKTMMEIALELA"
     gene            2393..3289
                     /gene="ispH"
                     /locus_tag="PLQ29_02130"
     CDS             2393..3289
                     /gene="ispH"
                     /locus_tag="PLQ29_02130"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPG85470.1"
                     /translation="MTVTRARTLGFCPGVRRAVETAERAAAAASASGSSGFVLGQIVH
                     NPRVSARLSGLGMETIADPDDAPGRLAGATVVIRSHGAAPEVFERLRAAGAEVVDATC
                     PRVAANQKAAASYASRGYLVVIAGDAGHGETLGVAGHAPGAVIVSSAAEATAVATSRP
                     LALIAQTTISETEYEAVREALRIPGRELVDVGGVCAASRERRSAIEELAGEVDAIVVV
                     GGKNSANTRRLAELAATLGVPTWHVENAAEIPDELGRYERVGLSAGASTPDEDIDEAE
                     ARLLELSQAASDDAASDAASRA"
     gene            complement(3286..3582)
                     /locus_tag="PLQ29_02135"
     CDS             complement(3286..3582)
                     /locus_tag="PLQ29_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85471.1"
                     /translation="MKRRVPLIVACVLAAILVSSCLPGDGRNTSANPAGFLSGFWHGI
                     VAPVSLVVQIFKPTIRVYEVANTGFWYDLGFWLAIGGGAGGGCAAAGRRKRYRA"
     gene            3810..4430
                     /gene="udk"
                     /locus_tag="PLQ29_02140"
     CDS             3810..4430
                     /gene="udk"
                     /locus_tag="PLQ29_02140"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0046108 - uridine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HPG85472.1"
                     /translation="MAEAKLIGICGGSGSGKTTIVRKISELVADFVFIPQDNYYKSAE
                     YVSNSNITAFNFDHPEAFDNELLWSQLSALKRMEPVEIPTYDFVHHRRAEETIKVTPR
                     KLVIFEGIMVFTNEKVRDLIDLKIFVDTPDDIRFIRRLSRDIKERGRTVDSVIDQYLN
                     VVRPGHFQFIEPTKRFADIIIPEGGANENALEVLVTFIKTILNSEG"
     gene            complement(4414..6447)
                     /locus_tag="PLQ29_02145"
     CDS             complement(4414..6447)
                     /locus_tag="PLQ29_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HPG85473.1"
                     /translation="MSNRPGHPSSPLQTIAYALLAAAIGLAFTAGVYAELAADRRDRL
                     EAGFAFDAAQRTELVREAFAHHGDALESIKRFFENSSGVTNGEFTRFASAWASVKGFR
                     SLVWAVPDDGSDGNPPGGGELRPVYRVPTLDSDAELAWLGLSAVRSAVAEARVSGRLN
                     ASELFELGDARYDPFVALCLPVYAAWDESGVRRFGLVIGLVSLPTVSNALISPELPTG
                     LPFSIRDYGAGPGAPEYRYASRIGAMPPPGHQYRRLSYPRILDFSGRSWFVEVRASTG
                     YVSGVSSDAGPEFFLFAGALASFVLGFAVHSLSRSRARAEASAAAAGEEAELFFQLGP
                     DMFMITELDGRIRRLNSEWTASLGYRVGELEGYLLIDFIHPDDREESIRVCRGVGDGI
                     ALEGYVNRVRTAAGDYRRVEWRARRAPGGGAVFVGARDVTERESMEERLRGSIVEREA
                     LIKEVHHRVKNNMQIISSLLELGSMDEADARFTAVAKEARGRIRSMALVHEQLYKRDS
                     ISSIDLGEYLRELAGRVVGEFASGAVDLVVDAGVVPLDIDAAIPCGLAVNELVTNALK
                     YAGAGGRRASLRISAREEGGWCSVAVEDDGPGFPDGAFERSMAGATLGLSLVSGLASQ
                     LRGELRASSGPGARVELRFPLAPRGAGPGLADSPSDSPSGRPVGAPSGVSPRS"
     gene            6605..>8075
                     /locus_tag="PLQ29_02150"
     CDS             6605..>8075
                     /locus_tag="PLQ29_02150"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG85474.1"
                     /translation="MNRITEHPVIDAGQPRPEVRFSFDGEPLAGYDGEAVSSALFAAG
                     VKRFSEHRKDGAPQGIFCANGQCSQCNVLVDGVVRKACVTALRAGMDVRTVHGVPGLK
                     ADDGAAPEARIETMRTEVLVVGGGPSGLAAAAELAERGFDVILADDKAALGGKLVLQT
                     HKFFGSEEDCYAGTRGYDIAALLEKKVRAYPNVRVLANSPVLAVYKDRKAGVYLDYGR
                     YALIDFQAIVVAAGAREKSILFPGNDLPGVYGAGAFQTLVNRDLVKAARRVFVVGSGN
                     VGLIGSYHALQAGIEVAGICEILPRVNGYKVHADKIRRMGVPIHLSTTVVSVEGDGRV
                     ERVTVADVDADYRPILETARSYEVDTVLVATGLSPCDELYRQAIRFGFAAVKAGDAEE
                     IAEASSAMFGGRIAALSLAKLLGRKVEIDAEWLAKREVLKSRPGEVIAREPVVPGDSW
                     KPVFFCTEEIPCNPCTTVCPTESIQLKARKGSILDLPYFA"
BASE COUNT         1399 a         2723 c         2754 g         1199 t
ORIGIN      
        1 cttcatgacc cagcccttgg tcttgcaggc gtccaggtgc tcggccttga agaaggggcc
       61 gacgtacttg gtcgggttga tcatggacgg gtcgtccttg tcgacgacga cctgggtgac
      121 gacggtgacg acctcggtct tgactccggc cttgagcagg gcgttcatca gcgactgctg
      181 gatcatgtag cctatcgagc cctgggtatc ggcgacgatc acgttgagcg agctcggggc
      241 cacctgcgcg gcgccggcct cgttgcggat caggtgcacc ccggcctggg ggccgttgcc
      301 gtgggtcagg acgacgcggt ggccttcctt gaccaggccg acgatcgcgg ccatgctctt
      361 gcgggtattc tcgaactgct gctcgatggt accctcggta ccctcctcga tcagggcatt
      421 gcccccgagg gcgacgacta tcgtcttctt gctcatctga accttccttc tgcggcggct
      481 cgccgggttg ctatcaggcg ccacgcgccc tacggcgaag cacggtccag tatagtaaaa
      541 cgccgacgcc gcgtacaatc gagggccctt atgcaatcca tattcggcgc gggccgccac
      601 gccgtatacg gggtgcatat cgcggcggcc caggataggc ttagaacgac gcgaacttgg
      661 tcttgtagcc cttcttgagc gagacgagct cgccggacac ggactccttg gagccgctct
      721 tggcccactc gtacttgacc aggcctcccg aggccttggg atcgacccac caggtgtagc
      781 ggtaggtcgt cttctcgtcc tcgtcgtagt aggtccactc gatgcgctcg gcgcggaagg
      841 tgccggcgcc gaccttgatc gtctccacgc ccttcttgta gccggagagg tcctcgagcg
      901 ccagctcgct ggtcggcgcc tcctcgggcg gggcggtctc cgagccgggc ttggccttgc
      961 cgctggtatc gaagacggcc gcctcgacgc gcttgacgtc ggcattgtag tagcggatcg
     1021 tacgggccat ctgatcctta tccatcagcg cctcgaattc ccacgactcc ccgtcaccgc
     1081 gccaggccag gtaccaccag gaatcgccgt tcggcagccg cttgagaaag gctcgctccg
     1141 cctcgacggc cgtgaagctc ccgtcctcgt cgacgctctt gatgcgccat acggcgccct
     1201 gcgtctcctc gagcgggtag aagtcggcgc cgaaaccgcc gatgaagaag atctgcgagt
     1261 acagcacgtt gaacatgacc ttacgctcga gcccgctcaa gccgacggcc gacgagacct
     1321 cggcggaggc cctggcctcg acggacgact gcaggtcggc gagcgggttg aagccgccta
     1381 tgccgggcag cgagaagcac gaggcgagta gcgccaggca gagcgccgat accagtccag
     1441 tcgggacggc gcggcgaacg attcttaatc cggtacgcat tcgataaacc tccgtggctc
     1501 ttccgatcgg cggcgcgatc ggtttgaata gtaagatatt gtaacgcgcc cttggtaaaa
     1561 gaccagcgaa aagtaggtga acgcgcatgg acgaaagggc cggccatgag gtaccttcgt
     1621 acgaggcgcg ccgcgggcgc cgccgccatc cgcgaacctg aggagactag accatggcca
     1681 caccccataa cgcagccaag aagggcgaca tagccccgac cgtgctcctg ccgggcgacc
     1741 cgctccgggc caagttcata gccgagacct tcctggagaa ccccgtctgc tacaacacgg
     1801 tgcgcaacat gttcggcttc acgggcagct acaagggcag gcccgtctcg gtccagggca
     1861 ccggcatggg cgtgccgtcg ctgtcgatct acgtgaccga gctgatccgg gagtacggcg
     1921 ccgagaacgt catacgcatc ggcacctgcg gctccatcca gcccgacgtg aaggtgcgcg
     1981 acgtcatcat ggcccagggt tcctgcaccg actccaacgt caaccggata cgcttcggcg
     2041 gcctcgacta cgcgccgata gccgacttcg gcctgatgcg gaaggcctgg aaggccgccg
     2101 aggatcgcgg cgtggcgctc aaggtgggca atatcctgac ctccgacacc ttctacggcg
     2161 acgaccccga ctcctggaag ctgtgggccg cctacaacgt gatggccgtg gagatggaga
     2221 ccgccgcgct atacacgata gccgccaagt tccgcaagcg ggcgctcacg ctcctgaccg
     2281 tgtccgacag catcgtgacg ggcgaggcga ccagcgccga ggagcgccag agcaccttca
     2341 agacgatgat ggagatagcg ctggagctgg catagggaga gggagaccga aggtgaccgt
     2401 gacgagggcc cgcacgctcg gcttctgccc gggcgtcagg agggcggtgg agacggcgga
     2461 acgggccgcc gcggcggctt cggcctcggg ctcgagcggc ttcgtgctcg gccagatcgt
     2521 gcacaacccc cgcgtctcgg cgaggctctc cggcctgggc atggagacga tagccgaccc
     2581 cgacgacgct cccggtcgcc tggccggcgc cacggtggtc atacggtccc acggcgccgc
     2641 cccggaggtc ttcgagcggc tccgggcggc cggcgccgag gtcgtcgacg cgacctgccc
     2701 gcgcgtggcc gccaaccaga aggcggccgc gtcctacgcc tcgcgcggct acctggtcgt
     2761 gatcgccggc gacgcgggcc acggcgagac cctcggcgtg gccggccacg cgccgggcgc
     2821 cgtcatcgta tcgagcgccg cggaggcgac tgcggtggcg acgagccggc ccctggccct
     2881 gatagcgcag acgacgatct cggagaccga atacgaagcc gtacgggagg cgctccgcat
     2941 ccccgggcgc gaactcgtcg acgtcggggg cgtctgcgcg gccagccgcg agcgccgctc
     3001 cgccatcgag gagctggccg gcgaggtcga cgcgatcgtg gtcgtcggag ggaagaactc
     3061 cgccaacacg aggcgcctgg ccgagctggc cgcgacgctc ggcgtaccga cctggcacgt
     3121 cgagaatgcg gccgagattc ccgacgagct cggccgctac gaacgggtcg gcctgtcggc
     3181 cggcgcctcg acgccggacg aggacataga cgaggccgag gctaggctcc tcgaactcag
     3241 ccaagccgcg agcgatgatg ccgcgagcga tgcggcaagc cgggcttagg cccggtagcg
     3301 cttacgccgg cccgccgcgg cgcatccgcc tccggcgccg cccccgatgg cgagccagaa
     3361 gcccaggtcg taccagaagc ccgtgttggc tacctcgtag acgcggatgg tcggcttgaa
     3421 gatctgcacg acgagcgaca cgggcgcgac gatgccgtgc cagaagcccg agaggaagcc
     3481 ggcaggattg gcgctcgtgt tcctaccgtc cccgggcagg caggacgata caaggatagc
     3541 ggctagaacg caggcgacga tcagcggaac gcgacgtttc attgggtatc ctttcatcga
     3601 aacggagtct acacctatat agcgcgccga gaggccgccc gcaaggctcg gtgcctatgg
     3661 aggtccctgg ggaggcccga gaccccggag cgcggccgcg ggacgcgcga ggaggctcgg
     3721 gccggggggc gcgtccggcc gcggcgaccg ggaggcggac gatggcgccc cccgttccca
     3781 aggcgcgagg agcgtgctat cctcgcgcca tggctgaagc gaaacttata gggatctgcg
     3841 gcggctcggg atccggcaag acgacgatcg tgcgcaagat atccgagctc gtggccgact
     3901 tcgtgttcat tccccaggac aactactaca agtcggcgga gtacgtctcg aactcgaaca
     3961 tcaccgcctt caatttcgac cacccggagg cgttcgacaa cgagctgctc tggagccagc
     4021 tctcggcgct caaacggatg gaaccggtcg agataccgac ctacgacttc gtgcaccacc
     4081 gcagggcgga ggagacgatc aaggtcacgc cgcgcaaact ggtgatcttc gagggcatca
     4141 tggtgttcac gaacgagaag gtgcgcgacc tgatcgacct gaagatcttc gtggacacgc
     4201 cggacgatat tcggttcatc cgaaggctgt cgcgggatat caaggaacgc ggtcgcacgg
     4261 tcgactcggt gatcgaccag tacctgaacg tggtacggcc gggacacttc cagttcatcg
     4321 agccgaccaa gcgcttcgcc gacatcatca tacccgaggg cggagcgaac gagaacgcgc
     4381 tggaggtgct cgttaccttc atcaagacga tactcaactc cgagggctga cgcccgaggg
     4441 agcgcccacc gggcggcccg acgggctgtc cgacgggctg tccgccaagc ccggcccagc
     4501 gccgcgaggc gccagcggga agcgcagctc gacacgggcg cccgggccgg acgacgcgcg
     4561 tagctcgccg cgtagctggg aggccaggcc cgataccagg ctcaggccga gcgtggcgcc
     4621 ggccatcgag cgttcgaagg cgccgtcggg aaagcccggg ccgtcgtcct cgaccgcgac
     4681 ggagcaccag ccgccctcct cgcgcgcgct gatacggagc gaggcgcggc gtccgcccgc
     4741 gccggcgtac ttgagggcgt tggtcacgag ctcgttgacc gccagcccgc agggtatggc
     4801 cgcgtcgatg tcgagcggta cgaccccggc gtccaccacg aggtcgacgg cgcccgaggc
     4861 gaactcgccg accacgcggc cggccaactc gcgcagatac tccccgaggt cgatgctcga
     4921 gatcgagtcg cgcttgtaca gctgctcgtg gaccagggcc atggagcgga tgcggccccg
     4981 ggcctccttg gccacggccg tgaaacgcgc gtcggcctcg tccatcgagc cgagctcgag
     5041 caggctcgag atgatctgca tgttgttctt gacgcggtga tgcacttcct taatgagcgc
     5101 ctctcgctcg acgatcgagc cgcgcagccg ctcctccatc gactcgcgct cggtcacgtc
     5161 cctggcgccg acgaagacgg cgccgccacc gggcgcgcgc ctggcccgcc actcgacgcg
     5221 tcggtaatcg ccggcggcgg tcctgacccg gttgacgtag ccctcgaggg cgatgccgtc
     5281 gccgacgcca cggcacacgc ggatcgattc ctcccggtca tcgggatgga tgaaatcgat
     5341 gaggaggtag ccctccagct cgccgacccg gtagcccagg gaggccgtcc actccgagtt
     5401 gagccgccgt atccggccgt ccaactcggt tatcatgaac atgtcggggc cgagctggaa
     5461 gaacagctcg gcttcctcgc cggcggcggc cgcactggcc tcggcccggg ccctggaccg
     5521 ggacagggag tggacggcga agccgagcac gaaggaggcc agggcgcccg cgaagaggaa
     5581 gaactccggg ccggcgtccg aggagacccc ggatacgtag ccggtggatg cccggacctc
     5641 gacgaaccag gatcgcccgg agaagtcgag tatcctgggg tacgataggc ggcggtactg
     5701 gtgccccggg ggcggcatgg cgccgatccg cgacgcgtag cggtactcag gggcgccggg
     5761 gccggccccg tagtcgcgga tcgagaacgg caggccggtg ggaagctcgg gggagatcag
     5821 cgcattcgaa accgtgggca ggctgaccag cccgatgaca aggccgaagc gccggacacc
     5881 cgactcgtcc cacgcggcgt agaccggtag gcagagcgcg acgaagggat cgtaccgggc
     5941 gtcgccgagc tcgaacagct ccgaggcgtt caagcggccg ctgacgcggg cctcggcgac
     6001 cgcggacctg accgccgaca ggccgagcca ggccagctcg gcgtccgagt cgagggtggg
     6061 cacgcggtag acgggcctga gctcgccgcc gccgggcgga ttcccgtcgg acccatcgtc
     6121 cggcaccgcc cagacgagcg agcggaagcc tttgaccgag gcccaggcgg acgcgaaacg
     6181 cgtgaactcg ccgttcgtca cgccggagga attctcgaaa aagcgcttga tcgactcgag
     6241 ggcgtcgccg tggtgggcga aggcctccct gaccagctcg gtccgctgcg cggcgtcgaa
     6301 ggcgaagccg gcctcgagcc ggtcgcgccg gtcggccgcg agctcggcat agacgcccgc
     6361 cgtgaaggcc aagccgatgg cggcggcgag cagcgcgtag gctatcgtct gcaatgggct
     6421 cgaaggatgt ccgggtcggt tgctcatcgc ccctactata atcgatgaaa cgtagaggcg
     6481 ctatatagac catgtatccg cgcacgggtt tcataaaata aaacccaggt gcgtatactg
     6541 aaatttctgt attgacagtt cgattccgac gtggttccat atccgcgcat cggagacaac
     6601 caccatgaac cgaatcaccg aacatccggt aatcgacgcc gggcaaccgc ggcccgaggt
     6661 ccgcttctcc ttcgacggcg agccgctcgc cggctacgac ggcgaggccg tcagctccgc
     6721 cctgttcgcc gccggcgtca agcgcttctc ggagcaccgc aaggacggag cgccgcaggg
     6781 catcttctgc gccaacggcc aatgctcgca gtgcaacgtg ctcgtcgacg gcgtcgtgcg
     6841 caaggcctgc gttaccgcgc tccgggccgg catggacgtg cgcaccgtgc acggcgtgcc
     6901 cgggctcaag gccgacgacg gcgcggcgcc cgaggcccgg atcgagacga tgcggaccga
     6961 ggtcctggtc gtcggcggcg gcccgtccgg cctggcggcg gcggccgagc tggccgagcg
     7021 cggtttcgac gtgatcctgg ccgacgacaa ggccgccctc ggcggcaagc tcgtgctaca
     7081 gacccataaa ttcttcggtt cggaggagga ctgctacgcc ggcacgcgcg gctacgacat
     7141 agccgccctg ctcgagaaga aggtccgcgc ctacccgaac gtgcgcgtac tggccaactc
     7201 cccggtgctc gccgtataca aggatcgcaa ggccggcgtc tacctggatt acgggcgcta
     7261 cgccctgatc gacttccagg ccatcgtcgt ggcggccggc gcccgcgaga agtccatact
     7321 gttccccggc aacgacctgc cgggcgtcta cggcgccggc gccttccaga ccctggtcaa
     7381 ccgcgacctg gtcaaggccg cgcgccgcgt gttcgtcgtc ggctccggta acgtgggcct
     7441 catcggctcg taccacgccc tgcaggccgg catcgaggtc gcggggatct gcgagatcct
     7501 gcccagggtg aacggctaca aggtccacgc cgacaagatc cggcgcatgg gcgtgccgat
     7561 ccacctgtcg accaccgtcg tgtcggtcga gggcgacggc agggtcgagc gcgtgaccgt
     7621 ggccgacgtc gacgccgatt accggcctat cctcgagacg gcgcgcagct acgaggtgga
     7681 caccgtgctc gtcgcgacgg ggctgtcccc ctgcgacgag ctgtaccgcc aggcgatacg
     7741 cttcggtttc gccgcggtca aggccggcga cgccgaggag atagccgagg cctcgagcgc
     7801 catgttcggg ggccgcatcg ccgccctgtc cctggccaag ctactcggac gcaaggtgga
     7861 gatcgacgcc gagtggctcg ccaagcgcga ggtgctcaag agccgacccg gcgaggtgat
     7921 agcgcgcgag ccggtcgtgc ccggcgactc gtggaaaccg gtgttcttct gcaccgagga
     7981 gataccgtgc aacccctgca ccacggtctg cccgaccgag tccatacagc tcaaggcgcg
     8041 caagggctcg atcctggacc tgccctactt cgcgg
//
LOCUS       DAOPHV010000046         7850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_11775_length_7850_cov_4.139448, whole genome shotgun sequence.
ACCESSION   DAOPHV010000046 DAOPHV010000000
VERSION     DAOPHV010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7850
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11775_length_7850_cov_4.139448"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..321)
                     /locus_tag="PLQ29_02155"
     CDS             complement(88..321)
                     /locus_tag="PLQ29_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HPG85475.1"
                     /translation="MIEAILAIVVAASIYAILARDLLHGVIALSVTSVLSASIFFLAS
                     APDVAITEAAVGAGLSTIIFVWAIRNTERTDRS"
     gene            complement(314..685)
                     /locus_tag="PLQ29_02160"
     CDS             complement(314..685)
                     /locus_tag="PLQ29_02160"
                     /inference="COORDINATES: protein motif:HMM:NF015302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HPG85476.1"
                     /translation="MSQALDAVNLARDLAGMALCAVASVFAIAGTLGMLLQKDPYTRL
                     QASSLVGTTATFSVLLAALVTAPSVAIAARMVVIIAFFLISNPTTTHIIAKYAWNSGI
                     APRTAPRARKPGAKVAGDDDD"
     gene            complement(682..969)
                     /locus_tag="PLQ29_02165"
     CDS             complement(682..969)
                     /locus_tag="PLQ29_02165"
                     /inference="COORDINATES: protein motif:HMM:NF015993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HPG85477.1"
                     /translation="MMAVAFDWTIRIFAACAMAMTLRLAIGPTTVDRLTTLAAVSAIV
                     LAILAISGAMGARAAFLDVALVYDIFGFLGILAIATYTRASRSGSPPEAPR"
     gene            complement(966..1478)
                     /locus_tag="PLQ29_02170"
     CDS             complement(966..1478)
                     /locus_tag="PLQ29_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HPG85478.1"
                     /translation="MTPRKLWTLVRVAATFGFLCGVWLLFSADNGWLSILAGASCAAL
                     IALASYDTFIDEHEAGKNMVFPRLLPALAYPARLVAAMYSSSFATLRTLMTGSAAPRV
                     VHFRTRLKSDLARVVLAEAITFTPGTIVIDLDEDHLIVHWLNSTTRHSRRAGETVKGR
                     LESIIGGMWV"
     gene            complement(1484..1939)
                     /locus_tag="PLQ29_02175"
     CDS             complement(1484..1939)
                     /locus_tag="PLQ29_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPG85479.1"
                     /translation="MMRFADVLEPGRIRVGVEARDKAGALRAASELLSPSGDAAGAAA
                     VEMAMGARETLSSTGVGNGVAIPHALIDEAGSLSMAVLRLGSPVDFGASDGRPVDLVF
                     AIVGPRDGAGEHLRILSKLIRVLHDPAFRVAAREAADGRALGELIMGKG"
     gene            complement(1936..2301)
                     /locus_tag="PLQ29_02180"
     CDS             complement(1936..2301)
                     /locus_tag="PLQ29_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85480.1"
                     /translation="MKLLVFILNKEELLEEVLEAYVEAGLPGATILDSEGLGYFLAYE
                     VPLFAGFKEFLKGNKPYNKTILSVVKDDKAVERLVPILDEITGGISQPGTGVMFTIPV
                     EWGYGFAEPRSSDLKEPTV"
     gene            2412..3704
                     /locus_tag="PLQ29_02185"
     CDS             2412..3704
                     /locus_tag="PLQ29_02185"
                     /inference="COORDINATES: protein motif:HMM:NF019244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HPG85481.1"
                     /translation="MDTTVYSARHHDFEGDSRRGKKLVAALALYLLAIGLTFSGFDPA
                     RLLNGLRVIIVSPDLLIDDYTSIAGVGPAFFNAALCTLVIVLLLKRSRVEVTGPVIAG
                     VFTVAGFSLFGKNIANILPGLLGVVLYAKANNRPFSESVIVALFGTALAPLSSSVAFG
                     LGIPVPWNIPVAVAAGTATGFFLSPIARHVLDFHRGYNLYNMGFAAGFVGTVIMSTLK
                     AFGVPVEGEFHWAQLGPGYVAPCLIALFVALIVYGMIVDRDWRSSYRLLLGSSGRLVS
                     DFVRFYGLGATMVNMGLMGLMAMAAILLIGASWNGPMIGAALTIVGFSAFGKHPRNAL
                     PPMLGVAFMASLSRYGLSSPSSQLAVLFSSTLAPVTGEYGFVPGFISGMLHLVLVQVV
                     GSLHGGLDLYNNGFSGGLAAGLFVPVLEWIREWRKHEA"
     gene            3694..4065
                     /locus_tag="PLQ29_02190"
     CDS             3694..4065
                     /locus_tag="PLQ29_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85482.1"
                     /translation="MKHDAAQLMRVAEEVLKYFYNKGARSVKVSYDFGDQRSFCAVHA
                     KDLVLSPEDEAYIQNVFEAPVQPEIASYYGILVGRRRDEQEMQLVGTMADLEDLKNEE
                     GLGVVIVLSRREMEFRSSKKG"
     gene            4145..4717
                     /locus_tag="PLQ29_02195"
     CDS             4145..4717
                     /locus_tag="PLQ29_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPG85483.1"
                     /translation="MALTSRVATKVARRVVAAEGLVAAIGLVAAIGLVALLASMALAC
                     VPSGGRDEPAEAAAPRPSGKPQSGLATATLRSGAAEIVVELAVSPEEQEIGLMHRTEL
                     ADGRGMLFVYAEDRRLAFWMKHTLIPLSIAYISSDGVIREIRDMEPLSLAPVESERYV
                     RYALEAPLGWFGRVGLAPGDRFDLSGLPGR"
     gene            4769..5254
                     /gene="def"
                     /locus_tag="PLQ29_02200"
     CDS             4769..5254
                     /gene="def"
                     /locus_tag="PLQ29_02200"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPG85484.1"
                     /translation="MDIYTIGADVLRGNAAPVERFDDEFAALIERMFATMKQGKGVGL
                     AAPQVGIPQRFFVMQIDGDKQRVFVNPEIVETSLELDSFEEGCLSIPGVYADLDRPAE
                     IRVQAWNERGRRFVLGADGFLARVIQHELDHLNGVLFVDRLPERTRDRLLKQYEKRMR
                     A"
     gene            5260..6213
                     /gene="fmt"
                     /locus_tag="PLQ29_02205"
     CDS             5260..6213
                     /gene="fmt"
                     /locus_tag="PLQ29_02205"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPG85485.1"
                     /translation="MRVLFAGSPEIALPALEAIGARHELVGVLTNPPSAKGRGLCFSC
                     TPVAEAATALGVPVLSPERLGPAEREAVSSLRPELLAVFAYGRIFGPKFMALFPLGGI
                     NAHPSLLPRWRGCAPIPYAILNRDSVTGVCVQRVAPRMDAGDILARREIRLDGRETTD
                     SLSAVSATVGAELLLEAMAAIEAGTAVGEPQDEAAATYCGALAKDSGLVDWSSPAIDL
                     DARVRAFHPWPGAYTGFGGERLALLEAAPYPDEPGRAEPGTVLAVDKSKGIMVQTGRG
                     LIAVSRLQLRGKKPMDFKDFANGARGLVGTRLDAPPPSAAR"
     gene            6232..7242
                     /locus_tag="PLQ29_02210"
     CDS             6232..7242
                     /locus_tag="PLQ29_02210"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPG85486.1"
                     /translation="MDLRSMFNFKKLDQLDPRHAKAAIVSLVSLVVLMGLSGLIAFFL
                     ALRGEEQTLVPNVVDMELSAALVKLQDKELYPRVSLRFSGDPETRGRILEQDPQPGAI
                     VKAGRRIALVVSRGAAQEAVGDYIGQTVDEVKIHLQTVFGSTRQLITVKEPPVYVYDA
                     SPAGTILEQSPAPNVELSGPTQLSFVVSRGPEKAKVKVPDLVGLALSEAVLQIEKSGV
                     AFEFALRPTEGRERPGTVVAQLPAPGSLLDPGSPVSIVYGAPAKTEGRVSGLFQQALP
                     EYPYPLRAVLYAEPPTGARIPLISVDHPGRSFTMPYDLPVGSVLVLQVLNKVVARVEA
                     GR"
     gene            7239..>7850
                     /locus_tag="PLQ29_02215"
     CDS             7239..>7850
                     /locus_tag="PLQ29_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85487.1"
                     /translation="MSPRMIPSPRRRRKAPTYSVEIVRMALYDIGRSIDLKKAADLLP
                     GVPGMRLARRRDTPTTVTIPAPLCVDLRSGLKKVYSEDSIEGFAASARLYDEGVMSIV
                     VRLKVSATIEELHGIEAKEVSPTIRDLDSYIAERYQAVLEQVGPAVANRREQPESETY
                     LAYCFADVGSAPEDFIKENELAFTRLQETHDHLENAERSGPGHQ"
BASE COUNT         1276 a         2605 c         2650 g         1319 t
ORIGIN      
        1 cgtcggacat gacgaccggc gccagaaccg ccagcgccaa ggacgccgcg gccagaccgg
       61 tcgcggccga gagcagcttg ccgcgcctca cgagcggtcc gtccgttccg tattgcgtat
      121 cgcccatacg aagatgatgg tcgacaggcc ggcgcccacc gccgcctcgg ttatggcgac
      181 gtccggggcc gaggccagga agaatatcga cgccgatagg acgctggtca ccgacagcgc
      241 gatgacccca tggagcaggt ctctggcgag gatggcgtag atcgaggccg ccaccacgat
      301 ggcgagtatg gcctcaatca tcgtcgtctc ccgccacctt cgcccccggc ttcctggccc
      361 gaggcgccgt ccgcggcgct atgcccgagt tccatgcgta cttggcgatg atatgagtcg
      421 tggtagggtt cgatataagg aagaacgcga tgatgacgac catgcgcgcg gctatcgcca
      481 cggacggcgc cgtaacgagc gccgccagta atacagaaaa cgtggcggtc gtgccgacga
      541 gcgacgaagc ctggagcctc gtatagggat ccttctggag tagcatgccg agggtcccgg
      601 cgatcgcgaa gaccgaggcc accgcgcaca acgccatgcc cgctaggtcc ctggccaggt
      661 tgaccgcgtc gagggcttgg ctcatcgtgg cgcctccggc ggcgacccgg aacggctcgc
      721 cctggtatag gtggcgatgg ccaggatgcc gaggaagccg aagatgtcgt agacgagggc
      781 cacgtcgagg aaggccgccc tggcgcccat ggctccgctg atggcgagga tggccagcac
      841 gatggccgag acggccgcga gcgtggtcag gcggtcgacc gtcgtcgggc caatcgccag
      901 gcgcagcgtc atggccatgg cgcaggcggc gaagatccgg atggtccagt cgaaggcgac
      961 ggccatcata cccacatgcc cccgattatc gattccagtc ggcccttcac ggtctcgccc
     1021 gcgcgcctcg agtgcctggt cgtggagttg agccagtgca cgatcaggtg gtcctcgtcc
     1081 aggtcgatga cgatggtgcc cggcgtgaag gtgatggcct cggccagtac gacgcgcgcg
     1141 aggtccgatt tcagccgggt cctgaagtgc acgaccctcg gggcggccga gccggtcatg
     1201 agcgtccgta gcgtggcgaa gctggacgag tacatcgcgg cgaccagcct ggccggatac
     1261 gcgagcgccg gaaggagcct cgggaaaacc atgttcttgc cggcctcatg ctcgtcgatg
     1321 aaggtgtcgt acgaggccag tgcgatcagg gccgcgcacg aggcgcccgc caggatggat
     1381 agccagccgt tgtcggccga gaagaggagc catacgccgc acaggaagcc gaaggtcgcg
     1441 gcgacgcgaa cgagcgtcca gagcttgcgg ggcgtcatgg ggctcagccc tttcccatga
     1501 tcagctcgcc cagggcccgg ccgtcggcgg cttccctggc tgccaccctg aacgccgggt
     1561 cgtgcaggac gcgaatgagc ttcgagagga tgcgcagatg ctcgcccgcg ccgtccctgg
     1621 gcccgactat ggcgaatacg aggtcgacgg gccggccatc ggaggcgccg aagtccaccg
     1681 gcgagccgag cctcaggacg gccatggaca ggctcccggc ctcgtctatc agggcgtgcg
     1741 gtatggccac cccgttaccg acgccagtgg atgaaagcgt ctcccgcgcg cccatggcca
     1801 tctcgacggc ggcggctccg gccgcgtcgc ccgatgggct gagcagctcg ctcgccgccc
     1861 gtagggcgcc ggccttgtcc cgggcctcga cgccgaccct gatccggccc ggctcgagca
     1921 cgtccgcgaa acgcatcata ctgtcggctc cttcaggtcg ctggagcgcg gctcggcgaa
     1981 gccgtagccc cattccaccg gtatggtaaa catgacgccg gtccccggct gggagatgcc
     2041 cccggtgatc tcgtcgagta tcggcacgag gcgctcgacg gccttgtcgt ccttgacgac
     2101 cgacaggatc gtcttgttgt agggcttgtt gcccttgagg aattccttga agccggcgaa
     2161 tagcggtact tcataggcca ggaaatagcc gaggccctcc gagtcgagga tggtggcgcc
     2221 cggtaggccc gcctcgacgt aggcctcgag tacctcctcg aggagctctt ccttgttgag
     2281 tatgaacacg agtagcttca tggatccagt agtatcggcc ggtccggcac gggctgtcaa
     2341 ggaaaagctg ccgagaactc ccgtacgtca agcattgacg gcggggatgc ctacgctata
     2401 gaaaagatgg gatggatacg actgtatact cggcgcggca ccacgacttc gagggcgact
     2461 cacgacgcgg gaaaaagctc gtcgcggccc tcgcgctcta tctgctggcc ataggcttaa
     2521 ccttctccgg cttcgacccg gcccggttgc tgaacggcct gcgcgtcatc atcgtttcgc
     2581 ccgacctgct catagacgac tatacgagca tcgcgggcgt agggcccgcc ttcttcaacg
     2641 cggccctctg cacgctcgtg atcgtcctgc tcctgaaacg ctcgagggtc gaggtgaccg
     2701 ggcctgtcat agccggcgta ttcacggtcg ctggcttctc cctgttcggc aagaacatag
     2761 cgaatatcct gcccggtctg ctcggcgtcg tgctgtacgc caaggcgaac aatcggccgt
     2821 tcagcgagag cgtcatcgtg gccttgttcg ggacggcctt ggcgcccctg tcgagcagcg
     2881 tcgcgttcgg gctgggcata cccgtcccgt ggaacattcc ggtcgcggtc gccgccggta
     2941 cggccaccgg cttcttcctc tcgcctatag cccggcacgt actcgacttc caccgcggct
     3001 acaacctcta taacatgggc ttcgcagccg ggttcgtcgg cacggtcatc atgtcgacgc
     3061 tcaaggcttt cggcgtaccg gtcgagggcg agttccattg ggcccagctc gggccgggct
     3121 acgtcgcgcc ctgcctgatc gccctgttcg tcgcgctgat cgtctacggc atgatcgtcg
     3181 accgcgactg gcgttcgtcc taccgcctgc tactgggatc ctcgggacgg ctcgtcagcg
     3241 acttcgtccg cttctacgga ctcggcgcga cgatggtcaa tatggggctg atggggctga
     3301 tggcgatggc cgcgatcctg ttgataggcg cctcgtggaa cggcccgatg ataggcgccg
     3361 cgctgaccat cgtcggattc tccgcgttcg ggaagcaccc gcgcaacgcc ctgccgccga
     3421 tgctcggcgt ggcgttcatg gcctcgctgt cccgttacgg cctgtcgtcg ccgtccagcc
     3481 agctggctgt gctcttctcg agcaccctgg ctcccgttac gggcgagtac ggcttcgtgc
     3541 cgggcttcat ctccgggatg ctgcacctcg tgctcgtgca ggtggtcgga tcgctgcacg
     3601 gcgggctcga cctgtataac aacggcttct ccggaggcct ggcggccggc ctcttcgtcc
     3661 ccgtgctcga gtggatccgc gaatggagga agcatgaagc atgacgcagc gcagttgatg
     3721 cgggtggcgg aggaagtgct caaatatttc tataacaagg gcgccaggtc cgtaaaggta
     3781 tcgtacgact tcggtgacca gcgctcgttc tgcgccgtgc acgcgaagga cctggtcctg
     3841 tcgcccgagg acgaggcgta catccagaac gtgttcgagg cgccggtgca gcccgagata
     3901 gcgagctatt acggcatcct ggtcggccgc aggcgcgacg agcaggagat gcagctggta
     3961 ggcaccatgg ccgacctcga ggatctgaag aacgaggaag gcctcggcgt cgtcatcgtg
     4021 ctgagccgga gggagatgga gttccgatcg tcgaagaaag ggtagcttgc cccgcgggcg
     4081 ctaacggttt atgctcgcct ctcgggatgg aggtgccatg tatcaggtcg gtcaaaggcg
     4141 ttcgatggcg ctaaccagcc gcgtcgcaac caaggtcgcg cggagagtcg tcgcggccga
     4201 aggcctcgtt gcggctatag gcctcgttgc ggctataggc ctcgtcgcgc tgctcgcgtc
     4261 gatggcgctc gcctgcgtgc cgtcgggcgg gcgtgacgag ccagccgagg ccgccgcgcc
     4321 gaggccgtcc ggcaagccgc agtcgggcct ggcgactgcc accctacgct cgggcgcggc
     4381 ggagatagtc gtcgagctgg cggtcagccc cgaggagcag gagatagggc tgatgcatcg
     4441 cacggagctg gctgacggcc gcggcatgct gttcgtctac gccgaggacc gcaggctggc
     4501 gttctggatg aagcataccc tgatcccgct atcgatcgcc tatatctcct cggatggcgt
     4561 catccgcgag atccgcgaca tggagcccct gtcgctcgcc ccggtcgaga gcgagcgcta
     4621 cgttcgctac gccctcgagg cgccgctcgg ctggttcggc cgcgtcgggc tcgcccccgg
     4681 cgaccgcttc gacctgtccg ggctgcccgg tcggtagtcg aggcggcggc gaagccagta
     4741 tgttcatagt aggagagcca aggccatgat ggatatatat acgataggag ccgacgtgct
     4801 acgcgggaac gccgccccgg tggaacgctt cgacgacgag ttcgcggcgt tgatagagcg
     4861 gatgttcgcg acgatgaagc agggcaaggg cgtcggcctg gccgcgccgc aggtcggtat
     4921 cccgcagcgc ttcttcgtca tgcagataga cggcgacaag caacgcgttt tcgtcaaccc
     4981 ggagatcgtc gagacgagcc tggagctgga cagcttcgag gaaggctgcc tctcgatacc
     5041 cggcgtgtac gccgacctgg accggccggc ggagatacgg gtgcaggcct ggaacgagcg
     5101 cgggcgccgt ttcgtgctgg gggcggacgg cttcctggcc cgggtcatcc agcacgagct
     5161 ggaccacctg aacggcgtcc tgttcgtcga ccggctgccc gagcgcaccc gcgaccgcct
     5221 gctcaagcag tacgagaagc ggatgagggc ctgacgccgg tgcgcgtcct gttcgccggt
     5281 agccccgaga tagccctgcc ggcgctcgag gcgatagggg cccggcatga gctggtcggc
     5341 gtgctgacca atccgccgtc agccaagggg cgaggcttgt gcttctcgtg cacgccggtc
     5401 gcggaggccg cgacggccct cggcgtgccg gtgctgtcgc ccgagcgcct ggggccggcc
     5461 gagcgggagg ccgtctcatc gctacggccc gagctgctcg ccgtcttcgc gtacggccgc
     5521 atcttcgggc ccaagttcat ggcgctgttc ccgctcggcg gcatcaacgc gcacccctcc
     5581 ctgctgccac gatggcgcgg ctgcgcgccg attccctacg ccatactgaa tcgcgactcg
     5641 gtcaccgggg tctgcgtaca gcgcgtcgcg ccgcgcatgg acgcagggga catcctggcc
     5701 cggcgcgaga tccgcctgga cgggcgcgag acgaccgata gcctatcggc ggtctcggcc
     5761 accgtcggcg cggagctgct gctcgaggcg atggcggcca tcgaggccgg tacggcggtc
     5821 ggcgaacccc aggacgaggc ggccgccacc tactgcggcg cgctcgccaa ggactcgggc
     5881 ctcgtcgact ggtcgtcgcc ggccatcgac ctggacgcgc gcgtgcgagc cttccatccc
     5941 tggccgggcg cttataccgg cttcgggggc gagcggctcg ccctcctcga agcggcgccg
     6001 tacccggacg agcccggccg ggcggagccc gggacggtac ttgcggtaga caagtcgaaa
     6061 ggtataatgg tacaaacggg acggggcctc atcgccgtgt cgcgcctcca gcttcgcggc
     6121 aagaagccca tggacttcaa ggacttcgcg aatggcgcgc gcggcttggt cggtacgcgc
     6181 ctggacgcgc ctccgccgag cgccgcccga taaaccggta cgggagcacg catggacctt
     6241 cgttcgatgt tcaacttcaa gaagctcgat cagctcgatc ccagacacgc caaggcggcc
     6301 atcgtgtcgc tcgtgtcgct cgtggtgctg atgggcttgt ccggcctcat cgcgttcttc
     6361 ctggccctgc gcggcgagga acagacgctc gtgccgaacg tcgtcgatat ggagctgtcc
     6421 gcggccctcg tcaagctcca ggacaaggag ttgtatccgc gggtatccct gcgcttctcc
     6481 ggcgaccccg agacccgagg gcgcatcctc gaacaggatc cgcagccggg agccatcgtc
     6541 aaggctggtc gccgcatcgc gctggtcgtc tccaggggcg ccgcccagga ggccgtcggc
     6601 gactatatcg ggcaaacggt cgacgaggtg aagatccacc tccagacggt gttcgggtcg
     6661 acgcgccagc tcataaccgt caaggaaccg ccggtctatg tctacgacgc cagcccggca
     6721 ggcaccatac tcgagcagtc gccggcgcct aacgtggagc tgtccggccc gacccagctg
     6781 agcttcgtcg tatcccgcgg ccccgagaag gccaaggtca aggtgccgga cctcgtcggc
     6841 ctggcgctga gcgaggccgt gctgcagatc gagaagtcgg gcgtggcctt cgagttcgcg
     6901 ctgcggccga ccgaggggcg cgaacggccc ggcaccgtcg tagcccagct gcccgcgccg
     6961 ggtagcctgc tcgaccccgg ctcgccggta tccatcgtct acggggcgcc cgccaagacc
     7021 gagggccgcg tctccgggct gttccagcag gccctgccgg agtatccgta ccccctgcgg
     7081 gccgtcttgt acgccgagcc cccgaccggc gccaggatcc cgctgatctc ggtcgaccat
     7141 ccgggccgtt ccttcacgat gccgtacgac ctgcccgtgg gcagcgtgct cgtgctccag
     7201 gtgctcaaca aggtggtggc tcgggtggag gcgggaaggt gagcccgcgg atgattccct
     7261 cgcccaggcg gaggcgcaag gccccgacct attccgtcga gatcgtccgc atggccctct
     7321 acgatatcgg ccgctccatc gacctcaaga aggcggccga cctgctgccc ggagttccgg
     7381 gtatgcgcct ggccaggcgc cgcgataccc cgacgaccgt tacgatcccc gcgccgcttt
     7441 gcgtcgatct gcgctcgggc ctcaagaagg tgtatagcga ggattccatc gagggcttcg
     7501 ccgcctccgc gcgcctgtac gacgagggcg tcatgtccat cgtcgtcagg ctcaaggtca
     7561 gcgccaccat cgaagagctg cacggcatcg aggcgaagga ggtttctccg accatccgcg
     7621 acctcgactc ctacatagcg gagcgctacc aggccgtgct cgagcaggtc ggccccgccg
     7681 tcgcgaaccg gcgcgagcag ccggagtcgg agacctacct ggcctattgc ttcgccgacg
     7741 tcggctctgc ccccgaggat ttcattaaag aaaacgagct cgcgttcacc aggctccaag
     7801 agactcatga ccatcttgag aatgccgaac gttccggacc aggccaccag
//
LOCUS       DAOPHV010000047         7821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_11835_length_7821_cov_4.290755, whole genome shotgun sequence.
ACCESSION   DAOPHV010000047 DAOPHV010000000
VERSION     DAOPHV010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11835_length_7821_cov_4.290755"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /gene="cysK"
                     /locus_tag="PLQ29_02220"
     CDS             <1..935
                     /gene="cysK"
                     /locus_tag="PLQ29_02220"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPG85488.1"
                     /translation="YAETITHLIGNTPLVRVKAEGAAPGAVVLAKLESFNPFSSIKDR
                     VGLAMIEAAERSGALKKGAVIVEPTSGNTGVALAAVAAAKGYRIILTMPETMSIERRK
                     LLQAYGAELVLTEGPKGMKGAIAKAQELVASTPGAYMPMQFDNPANPEMHARTTAEEI
                     WKDTDGKVDIVVAGVGTGGTVTGIAKTIKARKPAFKAIAVEPVDSPVLSGGAPGPHKI
                     QGIGAGFVPGVYDASLIDEVIRATHQDAGAAARRLAREEGLLVGISSGAALWAALGVA
                     RRPESAGKLIVAILPDSGERYLSTWLFEDPVEVT"
     gene            982..1725
                     /locus_tag="PLQ29_02225"
     CDS             982..1725
                     /locus_tag="PLQ29_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HPG85489.1"
                     /translation="MNFLSRYQRQIAFRGIGEPGQARLALARATIIGVGALGTAIANS
                     LCRAGFGFIRLVDGDRIEESNLQRQMLFDEDDAAAGRYKAEAAAARLGRVNSAALIEP
                     IVARADASNIEALVGDVDVVLDGSDNFEVRYLINDACRRLGVPWIYGGVLGDAGVTMN
                     VLPRGGPCLRCLVPEPPEPGSFATPETVGVLNQITAIIGSVEAVEAMKIVLRSPDVRR
                     SLLSVSLWDASFQEIAIEASEACPVCGRR"
     gene            1864..2901
                     /locus_tag="PLQ29_02230"
     CDS             1864..2901
                     /locus_tag="PLQ29_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HPG85490.1"
                     /translation="MHMADALVSPGVGGALWAATLGLGAYSIRKVTAEVDDKKITLMG
                     VMAAFVFAAQMVNFTIPGTGSSGHLGGGLLLAALLGPYAGFLAMGSVLAIQALCFADG
                     GLLAYGCNVFNLGFFTCFLAYPLIYQPIAGDGSSKTKSFIAAMVAAVVGLQAGAFGVV
                     IETMLSGKTELPFGAFVALMQPIHLAIGVVEGFVTAAVLAYVRSASPELLAPGATVAV
                     SSARPKALLGLILATLVVGIGLSWFASTDPDGLEWSMEKVSGMAELEVEGGAHETAAG
                     IQAVTAFLPDYGFKSAETEEAEPSWPAIDAGTSLSGVVGGVLALGLSALAGLAAFGLR
                     RKPRPAGTGKA"
     gene            2904..3722
                     /locus_tag="PLQ29_02235"
     CDS             2904..3722
                     /locus_tag="PLQ29_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPG85491.1"
                     /translation="MRDPSRTLAAFYTVDELSRRHSPVHDRHPLALSATTAAFMVVIA
                     SFGRREVSTILPLFLYPCLTLALADVRPAELFRRLASVAPLILAIAALSPVFETAPVR
                     VGPWTIAGGWLSFGSLVVKLTLTVTAAFLLVATAGMGGVARGLRAAGVPRLAVVVLQM
                     TYRYVALLLAEVTTMTRAHELRSGGRRGVDRSVLGPMIGGLLLRSYDRAGRVYDAMSL
                     RGFDGEYRVGQASRFGVPDTLYCLAWLGYFAIVRLVDLAEALGRIANRLAGGML"
     gene            3779..4480
                     /locus_tag="PLQ29_02240"
     CDS             3779..4480
                     /locus_tag="PLQ29_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG85492.1"
                     /translation="MLGGLSLSLKPGSKLALVGANGSGKSTLLGLVAGLLQPQSGSID
                     IGGLTLCKRNLKEARQRIGFVFQDPDDQLFTGSVYDDVAFGPRNGGLDQAAVRERADA
                     ALSTVGIADLADRPPFRLSGGEKRMAAIATVLSMDPGLLVLDEPSAALDPKARRRLIS
                     LLSSLSQSMLIATHDLELALELCDETAILFGGAVEAAGRSKDLLADEGLLDRCGLEAP
                     LSVRACPVCGKRAAR"
     gene            4532..5353
                     /gene="larE"
                     /locus_tag="PLQ29_02245"
     CDS             4532..5353
                     /gene="larE"
                     /locus_tag="PLQ29_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HPG85493.1"
                     /translation="MDSITKEQALVASLKKYGSLAIAFSGGVDSSYLCAIAVEALGSR
                     ALAITVDSPLLPRHELEDARRVAAFVGIEHVIMEEGDIDETVAANPPDRCYLCKRIKM
                     SAMLRVAEDRGLAALAEGSNIDDLSDYRPGRLANAELGIRSPLSDAGMTKDDIRELSR
                     RRGLATWDKPAFACLASRVPYGDRLSAPLLARIEAAEDYLRDLGFRQCRVRSHDGTAR
                     IEVAPDERVKFFDETMMDDVSGRLKRLGYDFVSLELEGYRMGSMNRALDTERTEL"
     gene            5350..6663
                     /gene="larC"
                     /locus_tag="PLQ29_02250"
     CDS             5350..6663
                     /gene="larC"
                     /locus_tag="PLQ29_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HPG85494.1"
                     /translation="MKESILYYDCFSGISGDMNLSALVGLGVPEDHLRAELEKLGLDG
                     WSLRLTRDSKHGIFGLRADVELAGHEHNHEHAHEHNHEHGHEHAHEHNHEHEHDHEHD
                     HEHAHRTLTDIKALIEASSLGQRVKANAISIFGVLADAEGKVHGKPASEVSFHEVGAV
                     DSIIDIVGAAICLEYLKPDVVMASSVELGGGFVRCQHGLIPVPAPAVVEVLRGAPVRS
                     GSVPFETTTPTGAAILATAVDEFTDDKRFSIVRTAYGVGHRDMPTPNLLRVFWAEREA
                     PPKTMAKSSYGVRSAAGAETEGVVLECNIDDMNPELHGHVMDALFAAGADDVWITPIV
                     MKKSRSAITLSALCKPAARESVAVAMLAETTSFGFRSYEVGKTALERSVSTVATSLGN
                     VRVKSALSGGKVVRSKPEFDDVKALAEANDLSLVDAYAIVLRELD"
     gene            6665..7423
                     /gene="larB"
                     /locus_tag="PLQ29_02255"
     CDS             6665..7423
                     /gene="larB"
                     /locus_tag="PLQ29_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HPG85495.1"
                     /translation="MEERRLSGILDDYKSGALDLDGAMTLLKDYPEADLGFARLDTQR
                     SVRVGFPEVIYCAGKADAHLRAILERLLQAGGPILATKASPEQYEAVRDLSGELEYAP
                     DARLIVARKPAVASEGSPYIAVVAAGTSDYPIAEEAAITASMFGQRVERVYDVGVAGL
                     HRLVRRLELIRGARVVVAVAGMEGALVSVIGGLVSAPVIAVPTSVGYGASFGGLAALL
                     AMLNACASGVSVVNIDNGFGAGYQAALINRIGGS"
     gene            complement(7420..>7821)
                     /locus_tag="PLQ29_02260"
     CDS             complement(7420..>7821)
                     /locus_tag="PLQ29_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPG85496.1"
                     /translation="CRVALVHGRSPRIISRVIAGEAVGTLFDADAALRNRQRWIKRAR
                     PKGEIAVDDGALAAMRERRSLLPRGVVSVSGTFERGDVVLIGGAAMAVTSLSSVELEA
                     ARGKRSSELGGRDGTGGSRVVARPDDVVFLD"
BASE COUNT         1311 a         2681 c         2534 g         1295 t
ORIGIN      
        1 aatacgccga gacgattacc catttgatag gaaacactcc cctggtacgg gtcaaagccg
       61 agggcgcggc cccgggcgcc gtcgtgctgg ccaagctcga gtcgttcaac ccgttctcca
      121 gtataaagga cagggtgggt ctcgcgatga tagaggccgc tgagcgctcg ggcgcgctaa
      181 agaagggcgc cgtcatagtc gagccgacct cgggcaacac cggcgtcgcc ttggccgccg
      241 tggccgcggc caagggctac cgcataatcc tgacgatgcc ggagaccatg agcatcgagc
      301 gccgcaagct gctgcaggcc tacggcgcgg agctcgtgct gaccgaaggc cccaagggta
      361 tgaagggcgc catagccaag gcccaggaac tcgtcgcgag cacgcccggc gcctatatgc
      421 ccatgcagtt cgacaacccg gccaatcccg agatgcacgc gcggacgacc gccgaggaaa
      481 tctggaagga taccgacggc aaggtcgata tagtcgtagc cggcgtcggc acgggcggga
      541 ccgtaacggg catagcgaag accatcaagg ccaggaagcc cgcgttcaag gctatcgcgg
      601 tagagcctgt agactcgccg gtactgtccg gcggcgctcc gggcccgcac aagatacagg
      661 gcataggcgc gggattcgta cccggcgtct acgacgcgtc gctgatagac gaggtgatac
      721 gggcgacgca tcaagacgcc ggcgccgcgg cgcgccgcct cgcgcgggaa gaaggcctgc
      781 tcgtaggcat atcgtcgggc gctgcgctct gggccgccct cggagtcgcg aggcggcccg
      841 agagcgcggg caagctgatc gtcgcgatac tccccgactc cggcgagcgc tacctgtcca
      901 cgtggctctt cgaggatccc gtcgaggtta catagtgtat actcgacgat aggccgaagg
      961 gcggccgggg ggcggaagac cgtgaatttc ttaagccggt accagcgaca gatagcgttc
     1021 aggggcatag gcgagccggg acaggcccgc ttggcgctcg cccgcgccac gatcataggc
     1081 gtcggggccc tgggtaccgc gatagccaac tcgctgtgcc gcgccggctt cggcttcata
     1141 cgcctcgtgg acggagaccg gatagaggag agcaacctgc agcgtcagat gctgttcgac
     1201 gaggacgacg cggcggctgg ccgatataag gccgaggccg ccgccgcccg cctgggccgc
     1261 gtcaactcgg ccgcgttgat cgagccgatc gtcgccaggg cggacgcttc gaacatcgag
     1321 gccctggtag gcgacgtcga cgtggtgctc gacggctccg acaacttcga ggtccgctac
     1381 ctgatcaatg acgcgtgccg ccgtctcggc gtgccgtgga tatacggcgg agtcctgggc
     1441 gacgcgggcg tcacgatgaa cgtgctaccc cgtggcggcc cctgcctgcg ctgcctcgtg
     1501 ccggaaccgc cagaacccgg ctcgttcgcc acgccggaga ccgtcggcgt cctcaaccaa
     1561 ataacggcta tcatcggctc cgtggaggcc gtcgaggcca tgaagattgt cctccgcagc
     1621 cccgacgtgc gccgctcgct cttgtccgtc agcctgtggg acgcgtcgtt ccaggagatc
     1681 gccatcgagg cgagcgaggc ttgcccggtc tgcggtcgcc gctgagcttt caccggcaca
     1741 gaattgaccg ttatcgattt catctctata ctcacggctc ggccatgaag gccgtgtaca
     1801 taccggttga accggtccgc cccggagacg aactcctcca tccactatcc tcaggagtct
     1861 ctcatgcaca tggctgacgc gctcgtctct cccggcgtgg gcggcgccct ctgggccgcc
     1921 acgctgggcc tcggcgccta ttccatccgc aaggttacgg cggaggtcga cgacaaaaag
     1981 atcaccctca tgggagtcat ggccgccttc gtcttcgccg cccagatggt caatttcacc
     2041 atccccggca cgggctcgag cggacacctc ggcggcggcc tgctcttggc cgcgctactc
     2101 ggcccctatg cgggcttcct ggccatgggg tcggtcctgg ccatccaggc cctctgcttc
     2161 gccgacggcg gcctcctggc ctacggctgc aacgtattca acctcggttt cttcacctgc
     2221 ttcctcgcct accccctcat ctaccagccc atagccggtg acggttcctc caagaccaag
     2281 tcattcatag cggccatggt tgccgccgtc gtcggcctcc aggccggcgc cttcggcgtg
     2341 gttatcgaga cgatgctgtc cggaaagacg gagctaccct tcggggcctt cgtcgccctg
     2401 atgcagccca tacacctggc cataggcgtc gtggagggtt tcgtgaccgc cgccgtcctg
     2461 gcctacgtcc gctcggcctc gcccgaactc ctggctccgg gcgccaccgt agccgtatcc
     2521 tccgcccgcc caaaggcgct cctggggctc attctagcga ccctggtcgt cggcatcggc
     2581 ctgtcctggt tcgcctccac cgatcctgac gggctcgagt ggtctatgga gaaggtctca
     2641 ggcatggccg agctcgaggt cgagggcgga gcgcacgaga cggccgccgg catccaggcc
     2701 gttaccgcct tcttgcccga ctacggcttc aaatcggccg agaccgagga agcggagccg
     2761 tcctggccgg ccatcgacgc tggcacgagc ctgtccggcg tcgtcggtgg cgtcctggcg
     2821 ctcggcttgt ctgccctcgc cggcctggca gcgttcggcc tgcgccgcaa gccacgcccg
     2881 gccggaacag gaaaagcttg aagatgagag acccgtcgcg gaccctggcc gcgttctaca
     2941 ccgtcgacga gctatcgcgg cgacatagcc cggtccacga caggcatccc ctggccctca
     3001 gcgcgacgac ggccgccttc atggtcgtca tagcctcgtt cgggaggcgg gaggtatcga
     3061 ctatcctacc cctgttcctc tacccctgcc tgactctcgc gctggccgac gtccgaccgg
     3121 ccgagctgtt ccgcaggctg gcctccgtag cgcctctcat cctggccata gccgccctca
     3181 gccccgtctt cgagactgcc ccggtcaggg taggtccctg gaccatcgcc ggaggctggc
     3241 tgtcctttgg ttcgctcgtc gtcaagctga cgctgaccgt gacggcggcc ttcctcctcg
     3301 tcgcgaccgc cggcatgggc ggcgttgcca ggggcttgcg cgcggccggc gtaccaaggc
     3361 tggccgtggt cgtcctgcag atgacctatc gctacgtcgc cctcctcctc gcggaggtga
     3421 cgactatgac acgggcccac gaactacgct cgggcggacg gcgcggggtc gatcgttccg
     3481 tcttgggtcc gatgatcggg ggcctcctcc tccgttcata cgaccgcgcc gggcgcgtat
     3541 acgacgccat gagcctacga ggcttcgacg gcgagtaccg cgtcggccag gcctcgcgct
     3601 tcggcgtgcc ggacaccctc tactgcctgg cctggctggg ctatttcgcc atcgtgcgac
     3661 tagtcgacct tgcagaggcg ctcgggcgga tcgctaaccg cctggcggga gggatgcttt
     3721 gaacgcctgc ggactcgatc tgaacgcgat tcgcttctcc tatccagacg ggaggcctgt
     3781 gctcggaggc ctgagcctga gccttaaacc tggctccaag ctggcgctgg taggcgccaa
     3841 cggctcgggc aagtcgacgc tgctcggcct cgtggcggga ctgctccagc cccagtcggg
     3901 tagcatcgac attgggggcc tcacgctatg caagcgcaac ctcaaggaag cgcgccagag
     3961 gatcggcttc gtctttcagg acccggacga ccagctgttc accggtagcg tctacgacga
     4021 tgtggccttc ggcccccgca acgggggact tgaccaggcc gcagtccggg aacgggctga
     4081 tgcggccttg agcacggtcg gcatagccga cctggccgac cggcctccgt tcaggctctc
     4141 gggcggcgag aagcgcatgg cggccatagc gacggtgctg tcgatggatc cgggcctcct
     4201 cgttctggac gaaccgagcg cggcgctcga ccccaaggcc aggcgccggc tcattagcct
     4261 cctgtcgagc ctgtctcaga gcatgctcat agcgacccac gacctcgagc tggccctgga
     4321 gctatgcgat gagacggcca tcctgttcgg aggcgccgtc gaggccgccg gaagatcaaa
     4381 ggacctacta gccgacgaag gtctcctcga tcgctgcggg ctcgaggcgc cgctatccgt
     4441 ccgcgcctgc cccgtctgcg gaaagcgggc ggcgcgctaa cgctcagcgt ggtatactcg
     4501 acccgccacg cacatcaaga aggactacga gatggatagt atcacgaaag aacaggccct
     4561 agtcgcctcg ctcaagaagt acggatcgct cgcgatcgcg ttctccggag gcgtcgattc
     4621 cagctacctc tgcgccatag cggtcgaggc gctcggttcc agggccctgg ccatcaccgt
     4681 cgactcgccg ctcctaccac gtcacgagct ggaggacgcg cgccgggtag ccgccttcgt
     4741 cggtatcgag catgtgatca tggaggaagg cgacatcgac gagaccgtcg ccgccaaccc
     4801 tcccgatcgc tgctatctct gtaagcgcat caagatgagc gccatgctga gagtcgcgga
     4861 agacagaggc ctcgcggccc tcgcggaagg ttccaatatc gacgatctct ccgactaccg
     4921 tcccgggcgc ctcgcgaacg ctgagctcgg tatccgctcg ccgctctccg acgccggcat
     4981 gaccaaggac gatatacgcg agctatcccg gcgccgcggc ctcgccacct gggataagcc
     5041 ggccttcgca tgcctggcct cgcgcgtgcc ctacggtgac aggctaagcg ctcccctcct
     5101 ggcgcgcatc gaggcggccg aggactatct gcgcgacctc ggcttcaggc aatgcagggt
     5161 caggagccat gacggcaccg cccgcataga ggtggccccg gacgaacggg tcaaattctt
     5221 cgacgaaacg atgatggacg acgtatcggg ccggctcaag cgactcggct acgacttcgt
     5281 cagcctcgag ctcgagggct atcgtatggg aagcatgaac agggcgctcg ataccgaaag
     5341 gacggaatta tgaaggaaag catcctatat tatgactgtt tctcgggcat ctccggcgac
     5401 atgaacctct cggccctcgt cggcctcggc gtcccggagg atcacctccg ggccgagctc
     5461 gagaaactcg gtttggacgg ttggtcgcta cgccttaccc gcgactcgaa gcacggcatc
     5521 ttcgggcttc gcgccgacgt cgagctggct ggccatgagc acaaccatga gcacgcccat
     5581 gagcacaacc acgagcatgg ccatgagcac gcccatgagc acaaccacga gcacgagcat
     5641 gatcacgagc atgatcatga gcacgcccac cgcacgctga ccgacataaa ggcgctgatc
     5701 gaggcgagct cgctcgggca gcgggtcaag gccaacgcta tatcgatctt cggggtgctg
     5761 gcggacgccg agggcaaggt ccacggcaag ccggcctcgg aagtcagttt ccacgaggtc
     5821 ggggcggtcg actcgatcat cgatatcgtc ggcgcggcca tctgcctcga gtacctcaag
     5881 cccgacgtgg tgatggcgtc gtccgtcgaa cttggcggcg gcttcgtgcg ctgccagcac
     5941 ggcctgattc cggtgccggc ccctgccgtc gtcgaggtgc tgcgcggcgc cccggtcagg
     6001 agcggatccg taccgttcga gacgacgacc ccgacgggcg cggcgatcct cgcgaccgcc
     6061 gtcgacgaat tcaccgacga caagcgcttt tccatcgtca ggacggccta cggcgttggc
     6121 caccgcgaca tgcctacgcc taacctgttg cgcgtcttct gggccgagcg ggaggcgccg
     6181 ccgaagacga tggccaagtc gagctacggc gtcaggagcg cggccggcgc ggagaccgag
     6241 ggcgtcgtgc tcgagtgcaa catcgacgac atgaatcccg agttgcacgg ccacgtgatg
     6301 gacgccctgt tcgcagccgg cgcggacgac gtctggataa cgccgatagt gatgaagaaa
     6361 tcgcgctccg ccataacgct atcggcgctc tgtaagccgg ccgctcggga gtcggtcgcc
     6421 gtagcgatgc tcgccgagac gaccagcttc gggttcaggt cgtacgaggt cggcaagacc
     6481 gcgctcgagc gcagcgtctc cacggtcgct acgagcctgg gcaacgtgcg ggtcaagtcg
     6541 gcgttgtccg gcggcaaggt agtgcgctcg aaacccgaat tcgacgatgt gaaggctcta
     6601 gccgaggcca acgacctgtc cctcgtcgac gcgtacgcca tagtgctcag ggagctggac
     6661 tgaggtggag gaacgacggc tgtcgggcat actcgacgac tataagtcgg gcgccctgga
     6721 cctcgacggg gcgatgaccc tacttaagga ttatcccgag gcggatctcg gtttcgcgag
     6781 gctggacacc cagcgctcgg ttcgggtcgg cttccccgag gtcatctact gcgccggcaa
     6841 ggccgacgcg cacctgcggg ccatactaga gcgactgctc caggcaggcg gccccatcct
     6901 agccaccaag gcttccccgg agcagtacga ggcggtcagg gacctatcgg gagagctcga
     6961 gtacgccccg gacgcgcggc tgatcgtcgc tcgcaagccc gcggtcgcgt cggaaggctc
     7021 gccgtatatc gcggtcgtcg ccgcgggcac ctccgactat cccatcgccg aggaggcggc
     7081 catcaccgcc tcgatgttcg gccaacgggt ggagcgcgtc tacgacgtcg gcgtcgccgg
     7141 cctgcaccgg ctcgtccggc gcctggagct tatacgcggc gcccgcgtcg tcgtcgcggt
     7201 cgccggcatg gaaggcgcgc tcgtcagcgt cataggaggg ctggtgtcgg caccggtgat
     7261 tgccgtcccg acgagcgtcg gctacggcgc cagcttcggc ggcctcgcgg ccctgctcgc
     7321 catgctcaac gcctgcgctt cgggcgtgtc ggtggtcaac atcgataatg gcttcggcgc
     7381 cggctaccag gcagccctga tcaaccggat aggcgggagc tagtccagga acacgacgtc
     7441 gtcgggcctg gccacgacgc ggctgcctcc ggtgccatcc cggccgccca actcagacga
     7501 acgcttgccc cgcgccgctt cgagctccac cgaagacagg ctcgtcacgg ccatcgcggc
     7561 gccaccgatc agcaccacgt ccccccgctc gaaggtgccg ctgaccgaca ccacgccgcg
     7621 cggcagtagc gagcgccgct cccgcatcgc ggcgagggcg ccgtcgtcca cggctatctc
     7681 gcccttgggc ctcgcgcgct tgatccagcg ctggcggttc cgcaacgccg cgtccgcgtc
     7741 gaacagcgtg ccgacggctt cgccggcgat gacgcgcgag atgatcctcg gcgacctccc
     7801 atggaccagc gcgacgcggc a
//
LOCUS       DAOPHV010000048         7753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_11975_length_7753_cov_3.979605, whole genome shotgun sequence.
ACCESSION   DAOPHV010000048 DAOPHV010000000
VERSION     DAOPHV010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11975_length_7753_cov_3.979605"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="PLQ29_02265"
     CDS             <1..1189
                     /locus_tag="PLQ29_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPG85497.1"
                     /translation="FSQHLPDGCWFGCTMACAHAVDGFELQTGPYKGQKVCVDGPEYE
                     TAAGVGSNLYVYEPEGILELNFYCDTYGIDTISFGTMTGFLMECWELGVLTPERTDGI
                     DLSWGNWKGAAQILHDLADGKRFGVLAGKGVKFLSEYFASEYGADARLMKDIALHGKG
                     LEQSEYISKESLAQQGGYYLTNKGPQHDEAWLIFMDMVNNKLPTFEAKAEALHYFPMF
                     RTWFGLQGLCKLPWNDVEPADNAKWPESNKVPEHVHNYIALYKAITGNDLDWDGLIRQ
                     SERVYNFQRAFNVRQGHGRREEDYPPYRAMGPVLEDEYLAREERYDKQMLEKIGVDPA
                     GKSIAERIRITREYRQKQYDSLIDAVFKRRGWTPQGTPTPEHLKEIGMDLPEVLEVVR
                     KRL"
     gene            1422..2060
                     /locus_tag="PLQ29_02270"
     CDS             1422..2060
                     /locus_tag="PLQ29_02270"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HPG85498.1"
                     /translation="MNLLAHVLLAWATLPDTEGQECTGALMADYFPGQDLAVFPPGIR
                     RGILQHRAVDAFTDAHPAFVELRRSIAAAGAPRFTAGILLDVFWDHALASEWERWGRQ
                     LCGMDLEPFCGEAYARVGRAAEYHNPGFASIFRFLESGAWLSAYARLDGIGETLAMLS
                     EKMSGRPDLGSCVRLLVELDRPVRSGFAAFWPELLAFARAWNDDAPVEALRS"
     gene            complement(2057..3073)
                     /locus_tag="PLQ29_02275"
     CDS             complement(2057..3073)
                     /locus_tag="PLQ29_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG85499.1"
                     /translation="MSAGLIPATNAAPERAPALELRSVVKHFPLRSLAAYGKAVHAMD
                     DVSLTLEAGGSLALVGESGSGKTTTASVIARLYERDSGELIVAGLPARPFRGRAESLA
                     YKKRVQLIFQDPFGSLNPTHTIRSILERPFLIHGLADGKADLEARLSDALEQVGLSPA
                     SEYLRRFPHELSGGQRQRVNIARTFAVEPSIVLADEPTSMLDVSIRMGVMNMMLDLKE
                     RKGVSYLYITHDLAGARYMCGSIAVMYAGMIMEMGPTDEVIRDAVHPYVRLLKSASPS
                     PEAGLVRTPLDAGGEIPSLIDPPPGCRFHPRCPYAEPRCRESVPGLADLGGGRRVRCV
                     LALR"
     gene            complement(3070..4080)
                     /locus_tag="PLQ29_02280"
     CDS             complement(3070..4080)
                     /locus_tag="PLQ29_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG85500.1"
                     /translation="MLEIRNLSAGYATAKGFVRAVDDVSFSLGRGEFLGVAGESGCGK
                     STLAYAIMRLLRDNARIESGSALFKGRDLAAMTAAEVNAHRWTDMAMVFQSAMNALNP
                     VLSVGEQLTDAVHAHRSVSRAEAEALAAEMLALVDIHADRLKSYPHQLSGGMRQRVMI
                     AMALILKPELLIMDEPTTALDVVVQRSIIEKIEELRRRLGFSVIFITHDLSLLVEISD
                     SLGIMYAGRMVEYGPTAELFNDPRHPYTEGLMNSFPSLTGPLRRMDGIEGRTPDLLSP
                     PPGCRFAPRCRYARPTCSAGDPPRLEPLGGGRLSACPYPRRGEAYGCAPAAPAAPEGG
                     SL"
     gene            complement(4097..5074)
                     /locus_tag="PLQ29_02285"
     CDS             complement(4097..5074)
                     /locus_tag="PLQ29_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG85501.1"
                     /translation="MIKDFFKTLVSNTKSLVGLCLLGFFVLVAILAPVIAPYPPKLDR
                     LETVVELGEPSLEEEILSEDYPSDARDVIVYRSTLTREYEKTVDYFPLRAKPSAAHPL
                     GTNHAGNDLFSQLVWGTRVSLTIGLLTGLFVTVLSLTLALLSGYLGGVADDVITFVTN
                     VMLVIPGLPLMIVVAAYVTMKGTLPIVLILGLTGWPYPTRLLRSQVLTLKNRDFVRVS
                     KSLGERTSFVVFREILPNMISIAMAEFFATSMAAILGEATLEFIGLGNVTVVSWGTIL
                     FWAQANGALLSGAWWWFLPPGICIALVGTSFVLINFAIDEISNPRLRKR"
     gene            complement(5071..6051)
                     /locus_tag="PLQ29_02290"
     CDS             complement(5071..6051)
                     /locus_tag="PLQ29_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG85502.1"
                     /translation="MKYFLRKLGLLAFTLFVSVSLNFFLPRMMPGDPARALLDKMENL
                     QPEQLDTIRAAFGLDTDDPLIVQYGRYLRNTLRGDLGTSFSRFPTPVSEVMRVALPWS
                     IGLMGICTVISFSVGTIVGIGAAWRRETKLASITVGFFSFVRSFPYFWLGLVFIYFFA
                     FKARIFPIGSAYDVEAARGSASWWLSVLRHGALPAMTIVVSSLGAWILTMRNNMINVL
                     AEDYITVAMAKGLPLQRIKSMYAAKNAILPSVTGFAMSLGFVIGGGLVTEMVFAYPGI
                     GYMLYQAVNAKDYPLMQAIFLFIAVAVLIANFAADIAVMILDPRVRDGAK"
     gene            complement(6132..>7753)
                     /locus_tag="PLQ29_02295"
     CDS             complement(6132..>7753)
                     /locus_tag="PLQ29_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPG85503.1"
                     /translation="ERFGGRRGRQLTFGQAPKRGGFLRMTPAKQGVLVQNFNPFSPAA
                     LESTVGCFYETLIYFNMVDGSANPWLAEKWSWSKDLKTITFTIRSGVKYNDGSAMTVD
                     DVLYSALLGKDNKALDLSGLWAEGLQTVVAKGNEVSFTFAEVNVTTLEKFGSLVVVPK
                     SVWSKVDPLTWTGNLNPVSTGPFMLEPGSFNEQSYRLVRNPHYWQLGKDKKPLPYIDG
                     IQYVTTTNEQVGFKLLAGEYDWAGYSLPNIDDYVKADPEHNKYWFPEGNLVFLYMNNL
                     KAPFDNVNVRKAIAAAISQRDITRKMSPSPAPATMSAVKSTFSEIAKGAMSKYDIRRD
                     VAKAKKALEAEGYRLNAKGVYEKGGQALSFKLYVPTGWTDWIGAAETVAGQLKDIGVE
                     AVITQAAWPDPYQFALEKGDWDFAISFISVGSTPHTQFTRWLHSSRFAPIGEKAMTFS
                     NMRYKNEAVDKALDAYRSEPSPVKQKQYMATVVEQFMKDTPCVPLFFNPTWFIYSTRN
                     FTGFPSPEDPYAWPIVNGMQKTPILLRLQKR"
BASE COUNT         1340 a         2518 c         2606 g         1289 t
ORIGIN      
        1 gttcagccag cacctgcccg acggttgctg gttcggctgc acgatggcct gcgcccacgc
       61 ggtcgacggc ttcgagctcc agaccggccc gtacaagggc cagaaagtct gcgtcgacgg
      121 ccccgagtac gagaccgctg ccggcgtcgg ctcgaacctg tacgtctacg agcccgaagg
      181 catcctcgag ctgaacttct actgcgacac ctacggtatc gacacgatca gtttcggcac
      241 gatgaccggc ttcctcatgg agtgctggga gctcggcgtc cttacgcccg agcgtaccga
      301 cggcatcgac ctgagctggg gcaactggaa gggcgccgcc cagatactgc acgacctggc
      361 cgacggcaag cgcttcggcg tcctcgcggg caagggcgtc aagttcctct ccgagtactt
      421 cgcctcggag tacggagccg acgcccggct catgaaggac atcgcgctcc acggcaaggg
      481 cctcgagcag agcgagtaca tttcaaagga atccctggcc cagcagggcg gctactacct
      541 gaccaacaag ggtccgcagc atgacgaggc ctggctgatc ttcatggaca tggtcaacaa
      601 caagctgccg accttcgagg ccaaggccga ggccctgcac tacttcccga tgttccgcac
      661 ctggttcggg ctccagggtc tgtgcaagct accctggaac gacgtcgagc cggcggacaa
      721 cgccaagtgg cccgagtcca acaaggtacc cgagcacgtg cacaactaca tcgccctcta
      781 caaggccatc accggcaacg acctggactg ggacggcctg atccgccagt cggagcgcgt
      841 ctacaacttc cagcgcgcct tcaacgtccg ccagggccac ggccgccgcg aggaggacta
      901 cccgccgtac cgcgcgatgg gtcccgtgct cgaggacgag tacctggccc gcgaggaacg
      961 ctacgacaag cagatgctcg aaaagatcgg tgtcgatccc gccggcaaga gcatcgccga
     1021 gagaatacgc atcactcgag agtaccgcca gaagcagtac gactcgctga tcgacgccgt
     1081 cttcaagcgc cgcggctgga ccccccaggg caccccgacg cccgagcacc tcaaggagat
     1141 cggcatggat ctgcccgagg tcctcgaggt ggtcaggaag cgcctgtaac catcgatccg
     1201 ggcgtctccc cctcctccgg ttacggagga gggggccgcg ctctccgctg caagcccaca
     1261 gccgagcgct tccagcgctc ggctgtgggt tttccgctac gatcgctgac gctagtccgc
     1321 ggccgctgta cgcgaacgtc ggggcgagcc gtccggcgtc ggccgccagg cttgccgccg
     1381 gcccgggtcg gcggctatga tggccggatc ggaggccatc tgtgaacctg ctcgcgcacg
     1441 tactgctcgc ctgggctacc ctccccgaca ccgaggggca ggaatgtact ggcgcgctga
     1501 tggccgacta cttccccggg caggatctgg ccgtctttcc gccgggcata cgtcgcggca
     1561 tactccagca ccgcgccgtc gacgcgttca ccgacgccca ccctgccttc gtcgagctgc
     1621 gccgctccat cgccgcggcc ggcgcgccgc gcttcactgc cggtatactc ctcgacgtgt
     1681 tctgggacca cgcgctcgcc tccgagtggg aacgctgggg ccgtcagctc tgcggcatgg
     1741 atctcgagcc gttctgcggc gaggcctacg ccagggtcgg ccgggcggcc gagtaccata
     1801 atccgggctt cgcctccatc tttcgcttcc tggagtcggg ggcgtggctg tcagcctacg
     1861 ccaggctcga cggcatcggc gagaccctgg ccatgctgtc cgagaagatg agcggccgcc
     1921 ccgacctggg cagctgcgtg cggctactcg tcgagctgga ccgtcccgtt cgctcaggct
     1981 tcgccgcgtt ctggccggag ctgctcgcct tcgctcgggc ctggaacgat gacgccccgg
     2041 tcgaggccct ccggtcctag cgcagggcca gtacgcaacg caccctgcgc ccgccgccca
     2101 ggtcggccag cccgggcacc gactcgcggc agcgcggctc ggcgtagggg cagcgcggat
     2161 ggaagcggca tccgggcggc gggtctatca gcgacggtat ctcgccccct gcgtcgagcg
     2221 gggtccggac caggccggcc tccggagagg gcgaggctga cttgagcaac cgtacgtagg
     2281 ggtgcacggc gtcccggatg acctcgtcgg tgggccccat ctccatgatc atgcccgcgt
     2341 acatcaccgc gatggagccg cacatatagc gcgcgccggc caggtcgtgg gttatgtaca
     2401 ggtatgagac gcccttgcgc tccttaaggt cgagcatcat gttcataacg cccatgcgta
     2461 tggacacgtc gagcatgctc gtcggctcgt cggccagcac gatggacggc tcgacggcga
     2521 aggtgcgcgc gatgttcacc cgctgccgct gcccgcccga cagctcgtgc gggaagcgcc
     2581 gcaggtactc cgaggcgggc gacaggccca cctgctccag cgcgtcggag aggcgcgcct
     2641 ccaggtcggc cttgccgtcg gccaagccgt ggatcaggaa gggccgctcc aggatgctcc
     2701 gtatcgtatg ggtcgggttg aggctgccga acggatcctg gaagatcaac tggactcgct
     2761 tcttgtaggc gagcgactcg gcgcggccgc ggaagggccg ggccggcagg ccggccacga
     2821 tcagctcgcc ggagtcgcgc tcgtacagcc tggctatgac gctggccgtc gtggtcttgc
     2881 ccgagccgga ctcgccgacg agggcgagcg agccgccggc ctcgagggtc aggctgacgt
     2941 cgtccatcgc gtgcacggcc ttgccgtagg cggctaggga gcgtagcggg aaatgcttga
     3001 cgaccgagcg caactcgagc gccggcgcgc gctccggcgc cgcgttcgtc gcggggatca
     3061 gtccggcgct cataacgagc ccccttctgg agcggctgga gcggctggag cgcagccata
     3121 cgcctcgccg cgcctcggat acgggcaggc gctcagccgg ccgccgccga gcggctccag
     3181 cctcgggggg tccccggccg aacaggtcgg cctggcgtag cggcaacggg gagcgaagcg
     3241 gcagcccggc ggcggggaca gcaggtcggg cgtccggccc tcgataccgt ccatccggcg
     3301 tagcgggccg gtcagcgacg ggaaggagtt catcaggccc tccgtgtacg ggtggcgcgg
     3361 atcgttgaag agctcggcgg tcggtccgta ctcgaccatg cggccggcgt acatgatgcc
     3421 cagggagtcc gatatctcca cgagcagcga gaggtcgtgg gtgatgaaga tgacggagaa
     3481 gcccagtcgc cgcctgagtt cctcgatctt ctcgatgatg gaacgctgga cgaccacgtc
     3541 cagcgcggtg gtcggctcgt ccatgatgag cagctccggc ttgaggatga gcgccatcgc
     3601 tatcatcacg cgctgccgca tgccgccgga cagctggtgc gggtagctct tgagccggtc
     3661 ggcgtggatg tccaccagcg cgagcatctc cgccgccagg gcctccgcct ccgccctgga
     3721 cacggagcgg tgcgcgtgca ccgcgtcggt cagctgctcg cccaccgaca gcaccgggtt
     3781 gagcgcgttc atcgccgact ggaacaccat ggccatgtcc gtccagcggt gagcgttcac
     3841 ctcggccgcc gtcatggccg ccaggtcgcg gcccttgaac agcgcggaac ccgactctat
     3901 gcgagcgttg tcgcgcagga ggcgcatgat cgcgtaagcc agcgtagact tgccgcaacc
     3961 cgactcgcct gccaccccga ggaattcgcc ccgcccgagc gagaaggaca cgtcgtccac
     4021 ggcgcgcacg aagcccttgg ccgtcgcgta gcccgcgctc aggttccgta tctccagcat
     4081 cgtatcgcgt cctgacctac ctcttccgta atcgcgggtt cgagatctcg tcgatcgcga
     4141 agttgatcag gacgaacgac gtcccgacga gcgcgatgca gatcccgggc ggcaggaacc
     4201 accaccaggc cccggacagc agggcgccgt tggcctgggc ccagaacagg atcgtgcccc
     4261 agctgaccac ggtcacgttg cctaggccaa tgaactccag cgtggcctcg ccgagtatgg
     4321 cggccatgct ggtcgcgaag aactcggcca tcgctatgga gatcatgttg ggcaggatct
     4381 ccctgaacac cacgaacgag gtgcgctcgc cgagcgactt ggataccctc acgaagtcgc
     4441 ggttcttcag ggtcagcacc tgggaacgca gcaagcgggt aggatacggc cagccggtca
     4501 gccccaggat cagcacgatc ggcagcgtgc ccttcatggt cacgtaggcg gccaccacga
     4561 tcatcagcgg caggccgggt atgacgagca tcacgttcgt gacgaaggtg atgacgtcgt
     4621 ccgcgacgcc gcccaggtag cccgagagga gggccagcgt cagggacagc acggtgacga
     4681 acaggccggt caggaggcct atcgtcagcg acacgcgcgt gccccagacc agctggctga
     4741 agaggtcgtt gccggcgtgg ttcgtgccga gcggatgggc cgcgctcggc ttggcgcgga
     4801 gcgggaagta gtcgacggtc ttctcgtact cgcgcgtcag ggtggagcgg tagacgatga
     4861 cgtcccgggc gtcgctcgga tagtcctcgg agagtatctc ctcctccagg gacggctcgc
     4921 cgagctccac gaccgtctcg agtcggtcga gcttcggcgg atacggcgcg atgacgggcg
     4981 cgaggatcgc caccagtacg aagaagccga gcaggcacag cccgaccagg ctcttcgtgt
     5041 tcgaaacgag ggtcttgaag aaatccttga tcatttggcc ccgtccctca ccctcgggtc
     5101 gaggatcatg accgcaatgt cggcggcgaa gttggctatg agcacggcca ccgcgatgaa
     5161 caggaagatg gcctgcatca gcgggtaatc cttggcgttg accgcctggt acagcatgta
     5221 gcctataccc ggataggcga aaaccatctc ggtgacgagc ccgccgccga tgacgaagcc
     5281 gagcgacatg gcgaagccgg tgaccgaggg caggatggcg ttcttggcgg cgtacatgga
     5341 cttgatccgc tggagcggca ggcccttggc catggccacg gtgatgtagt cctcggccag
     5401 cacgttgatc atattattgc gcatggtcag gatccaggcg ccgagcgaac tgacgacgat
     5461 ggtcatggcc ggtagggcgc cgtggcgcag caccgacaac caccaggagg cggagccgcg
     5521 cgcggcctcg acgtcgtagg cgctaccgat cgggaagatc ctcgccttga acgcgaagaa
     5581 gtagatgaac acgaggccga gccagaaata cgggaagctc ctgacgaacg agaagaagcc
     5641 gaccgtgatc gaggccagct tggtctcccg gcgccacgcg gcgccgatgc cgacgatcgt
     5701 gccgacggag aagctgatca cggtgcagat gcccatcagc cctatcgacc agggcagcgc
     5761 gacgcgcatc acctccgaga ccggggtcgg gaagcgcgag aacgaggtgc cgaggtcgcc
     5821 gcgcagggtg ttgcgcaggt agcggccgta ctggactatc agcgggtcgt cggtgtccag
     5881 cccgaacgcc gcccgtatag tgtccaattg ctccggctgc aggttctcca tcttgtccag
     5941 caaggcgcgg gcgggatcgc caggcatcat cctgggcagg aaaaagttga gcgacaccga
     6001 gacgaacagg gtaaaggcga gcagccccag tttccttagg aagtatttca acgcggtcgg
     6061 tctccaatgc cgccctctgc cggaggtagg tttccggaaa gggcggcgaa cggtcagtcg
     6121 ttagcgggag ctcagcgctt ctgcaggcgg agcaggatcg gggtcttctg cataccgttg
     6181 acgatgggcc acgcgtacgg atcctcgggg ctcgggaacc cggtgaagtt gcgagtgctg
     6241 tagatgaacc aggtcggatt gaagaacagg ggcacgcagg gcgtgtcctt catgaactgc
     6301 tcgacgacgg tggccatata ctgcttctgc ttcacgggac tcggctcgga gcggtacgca
     6361 tccagggcct tgtccacggc ctcgttcttg tagcgcatgt tgctgaaggt catggccttc
     6421 tcgcctatcg gcgcgaagcg gctggagtgg agccagcgcg tgaactgcgt gtgcggggtg
     6481 gagcccaccg agatgaagct gatggcgaag tcccaatcgc ctttctcgag cgcgaactgg
     6541 tacgggtccg gccacgcggc ctgggtgatc acggcctcca cgcctatgtc cttgagctgg
     6601 ccggccacgg tctcagcggc gccgatccag tcggtccagc cggtgggcac gtacagcttg
     6661 aacgagaggg cctggccgcc cttctcgtag acgcccttgg cgttgagcct atagccttcc
     6721 gcctcgaggg ccttcttagc cttggccacg tcgcgccgga tgtcgtactt ggacatcgcg
     6781 cccttggcta tctcggagaa ggtggacttt acggcgctca tcgtcgcggg ggccgggctg
     6841 ggcgacatct tgcgcgttat gtcgcgctgc gagatggccg cggcgatggc cttgcggacg
     6901 ttgacgttgt cgaacggggc cttcaggttg ttcatgtaca ggaatacgag gttgccctcg
     6961 gggaaccagt acttattatg ctcgggatcg gccttcacgt agtcgtcgat gttcggtagc
     7021 gagtagccgg cccagtcgta ctcgccggcg agtagcttga agcccacctg ctcgttggtg
     7081 gtggtcacat actggatacc gtctatgtac ggcaggggct tcttgtcctt gcccagctgc
     7141 cagtaatgag ggttccgtac cagccggtac gactgctcgt tgaaggagcc cggctcgagc
     7201 atgaaggggc cggtggagac ggggttcagg ttgccggtcc aggtcagggg gtcgaccttc
     7261 gaccatacgg acttgggcac gacgacgagc gagccgaatt tctcgagcgt cgtcacgttg
     7321 acctcggcga aggtgaagct gacctcgttg cccttggcga cgaccgtctg cagcccctcg
     7381 gcccagagcc cggacaggtc gagggccttg ttgtccttgc ccagcagcgc ggagtacagc
     7441 acgtcgtcca cggtcatggc gctaccgtcg ttatacttga cgcccgagcg gatcgtgaag
     7501 gttatggtct tcaggtcctt ggaccaactc cacttctcgg cgagccaggg attggccgag
     7561 ccgtccacca tgttgaagta gatcagcgtc tcgtagaagc agcctaccgt tgactcgagg
     7621 gcggccgggc tgaagggatt gaagttctgc accagcacgc cctgcttcgc gggcgtcatc
     7681 ctgaggaagc cgcctcgctt cggcgcctgg ccgaacgtga gctgtcggcc gcggcgcccg
     7741 ccaaaccgct cag
//
LOCUS       DAOPHV010000049         7673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_12167_length_7673_cov_4.136781, whole genome shotgun sequence.
ACCESSION   DAOPHV010000049 DAOPHV010000000
VERSION     DAOPHV010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12167_length_7673_cov_4.136781"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..1321
                     /locus_tag="PLQ29_02300"
     CDS             197..1321
                     /locus_tag="PLQ29_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPG85504.1"
                     /translation="MPNRNRLHGEEGRAYWSMVLPAFSIYLLVMAFPIVLSIVLSVSN
                     YSGGKMFGGEPWGFAGLKSYARVFSDPLFWNALKNNFYIVLLSVFGQLPLGFIFAYII
                     YRRLVKWPGFWQGVLYVPNIISVIVVGLLWQTIFSPNGPIAEVMNDLARAGFQSRLEA
                     LFASVGGFNVDDGLVSKILDMAGPAALDWFSDPVPELKELILSYEGAPLSELIGDLCN
                     LFVPRWSPEFLSKPNIAMLPVLFVILWMYTGMYLILFLANMQKIDSQVIEAARIDGAS
                     EGQVMRYVILPALSGTIVNSAILAISGSLSSFSLIFAMTGGGPSRVTEILSIYMYNNA
                     FLGRPNLPLANAISLVMVVISFILIGLTKMAEKRYGGKEE"
     gene            1321..2181
                     /locus_tag="PLQ29_02305"
     CDS             1321..2181
                     /locus_tag="PLQ29_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPG85505.1"
                     /translation="MSDEIKTTALGRIGGAAAKAAAYAMMLLFTAMTLYPIFWLIMNS
                     FKSTREFQVSQLAFPKEPTLSNYVEAWKMGDFGLLFPNSLIYTIGATVGIVFVSLLAG
                     FAFAKIASRATKPIYNGFVIGILITAQSLMIPLFLEVNLLGIYNTRFAVLLVYIGTGL
                     PIGVYLATEYIKAIPSALIESARIDGASFWRIFAKIIVPMSMPVAVTLSILNISNIWN
                     EFALINILVSRTELKSLPLGIYKFSGSLSTDYGKQFAALTIGMVPMLLFYIVFRKQIT
                     RGVAAGAVKG"
     gene            2200..3555
                     /locus_tag="PLQ29_02310"
     CDS             2200..3555
                     /locus_tag="PLQ29_02310"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HPG85506.1"
                     /translation="MTNDRTAQGRGFPEDFLWGCATSSYQIEGGAKDGGRGQSIWDAF
                     CREPGRVALGQSGEVACDSYRRWPEDVRLLSGLGVGAYRFSIAWPRVQPDGSGAVNPA
                     GLDYYSALVDALLAEGIESWVTLYHWDLPLALGERGGWTDRDTAYRFADYAGLVYERL
                     GDRVRHWTTLNEPWCSAFLGYEHGEHAPGLRDRRAALSATHHLLLAHGLAVEAYRATG
                     RRGEIGLVINPATPRPATRREADVQAAGRASVERTGLWLDPVFGRGYPEEYLKARGAT
                     MPTRPGDLERIAAAIDFVGVNYYNEDAVVAAPPTAERPDGYDMAPTARERTEMGWDVA
                     PQGLRRVLGFIADRWPVRALYVTENGAAYPDAAIRDGRAHDADRIAYYRGHIAACADS
                     IGDGVPLRGYFAWSLLDNFEWSHGYGKRFGLVSVDPMTMARRPKDSYYFYRDVIAGFL
                     E"
     gene            3631..4551
                     /locus_tag="PLQ29_02315"
     CDS             3631..4551
                     /locus_tag="PLQ29_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85507.1"
                     /translation="MKSSTISLCRKILYESLTTDAMVRFVRLVEPEYDLHERSGIPVT
                     IPITNQMAAERVVSDILADGRFIDFVEVLVKIDAEGYMGRSYPIKGLGQIVKILEQDG
                     FVLDRSTGQFFENTNERASPDWGRLHEGDERQTSILRLDVVDNSALVQRNRPECVDRA
                     FVALRSIVSRAVVSRFGRVWSWEGDGALAAFMLGRKERSAVLAGMEILNELFFFNRSG
                     NPLDSPLRVRIAAHSGLVRYSDSLMELLKNDAVKDAIQIEASATPADTFSASSNLFLS
                     IDRTIQDRFGPDRRSPIGDIRHYSVNLEKA"
     gene            4548..5549
                     /locus_tag="PLQ29_02320"
     CDS             4548..5549
                     /locus_tag="PLQ29_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85508.1"
                     /translation="MSVYLFSMDASPALSFADDRSAKAVAVLGLEALKSHRPERRDLS
                     YIDLRGLDVEAGRKAVQTLRRRCSGSSWGVFDPEGTVADPAALFFAGASDYIGPAVIR
                     AGVDAARFKAALASTRRRDSTAEGEAEAGSLAAAGCADGAPEGGFGGWKAIRPGSSYP
                     FCFVYATASSQANLKTSLGEAGYVAFRDRLRHIMQQGLAEADALLWMETDSSVLYLVP
                     PYAANASAAIEFCLRTLLGAPLIGYERLCLPFPLDFTFAIHSGTTEFEPPGKTGTIVS
                     EAVNFSYHLGVKRAEPGRVTISADAAGLAVPGRFSDLFVPAGSFEGRSILHSRRFWA"
     gene            5613..6929
                     /locus_tag="PLQ29_02325"
     CDS             5613..6929
                     /locus_tag="PLQ29_02325"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HPG85509.1"
                     /translation="MVTIEKVEAGAAAARAMPRLPRRIKDDALRLLALRLRERAPELF
                     EANEADVAAARASGASEAILTRLVYDGKKLEESIAMIDAVSRLPDPSGRTLESRRLDD
                     GLVLRRVSCPIGLIALIFESRPDALVQMAALAVKSGDAIIVKGGREAERTNRALAAIV
                     AAAGVDAGLPSGWLVQLETRSEVARLLALDGLVDLVIPRGSNEFVRHIMETSRIPVLG
                     HAGGVCHVYVHGDADIAMAVAMVVDSKAQYPAACNAVEVLLVDEAAARSALPELVGAL
                     ERADVALELCPRSMAVVGPGPTRAIKDDDDWSVEYLSLRMAVRVVGSLDEAIAHINRY
                     GSGHTDAIVCSSGEAAARFMAEVDSTSVYRNASTRFADGYRYGLGAEVGISTGKLHAR
                     GPMGLEGLQTYKWLLEGEGHIVSEYSSGARRFIHEDLAVGKGGIHG"
     gene            6922..>7673
                     /gene="proB"
                     /locus_tag="PLQ29_02330"
     CDS             6922..>7673
                     /gene="proB"
                     /locus_tag="PLQ29_02330"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPG85510.1"
                     /translation="MAKANSFLDARLAHEARAALASAKRIVVKIGTATITRQKGQAGR
                     ISDGPDPGGSGIDVEFLHRVAAELADLARDGRQPVLVTSGAVGMGARALGLEKKPKDT
                     ASKQACAAIGQPLLMEEYRRAFDVFGLTAAQLLVTREAWEDREAYLNLRSTVEALLER
                     RAVPVFNENDSVSTAEIAFGDNDRLSAYVASKIDADLLVILSDVDALYDADPRTDPGA
                     RPIPFVREIDPAMMSAAGGKGSEFSTGGMRTKL"
BASE COUNT         1290 a         2520 c         2484 g         1379 t
ORIGIN      
        1 gatggcctca tcgccatcgg tctcggtacc aaggccgccg cccaggtggc cgccgaaacc
       61 caggccgcct tcgagagctg gaaagcctcg aagtaactcg cttgaaaggg cgcgacgccg
      121 cctccggcgc cgcgcccgcc ttcgtcgacc tggcgcggcg cccgaggcgc gcggcgccgc
      181 cgcatgagag gatcccatgc cgaatagaaa ccgactccat ggcgaggaag gccgggccta
      241 ctggtccatg gtgttgccgg ccttctcgat atacctgctg gtgatggcgt tccccatcgt
      301 gctgtccatc gtgctgtccg tatccaatta ttccggcggg aagatgttcg gcggcgagcc
      361 ctggggcttc gccggcctca aatcgtacgc gagagtattc tccgatccgt tgttctggaa
      421 tgcgctgaag aacaatttct atatcgtgct gctgtcggtg ttcgggcagc tgccgctcgg
      481 cttcatcttc gcctatatca tctaccggcg cctggtcaaa tggcccggct tctggcaggg
      541 cgtcctgtac gtaccgaaca tcatctcggt catcgtggtc ggcctcctct ggcagaccat
      601 cttctcgccg aacggcccaa tcgccgaggt aatgaacgac ctggccaggg cgggcttcca
      661 gtcgaggctg gaggcgctgt tcgcctcggt cggcggcttc aacgtcgacg acgggctggt
      721 atcgaagatc ctggacatgg ccgggccggc cgcgctcgac tggttctcgg accccgtacc
      781 cgagctgaag gagctgatct tatcatacga gggggccccg ctatccgagc tcatcggcga
      841 tctgtgcaac ctgttcgtgc ctcgctggag cccggagttc ctgtccaagc cgaacatcgc
      901 catgctaccg gtgctgttcg tcatactgtg gatgtatacc ggcatgtacc tcatcctctt
      961 cctggcgaac atgcagaaga tcgactccca ggtgatagag gctgctcgca tcgacggcgc
     1021 cagcgagggt caagtcatgc gctacgtgat tctgccggcc ctgtccggca ccatcgtcaa
     1081 ctcggccata ttggcgatct ccggatcgct ttcgagcttc tcgctcatat tcgcgatgac
     1141 cggcggcggc ccgtccaggg ttaccgagat cctatccatt tacatgtaca ataacgcgtt
     1201 cctgggcagg ccgaacctgc cgctggccaa cgcgatctcg ttggtcatgg tcgtcatcag
     1261 cttcatcctg atagggctga ccaagatggc cgagaagcgc tacggcggca aggaggaatg
     1321 atgagcgacg agatcaagac caccgcgctc ggccggatag gcggggcggc cgccaaggcg
     1381 gcggcctatg cgatgatgct gctcttcacg gcgatgacgc tgtacccgat cttctggctg
     1441 atcatgaatt ccttcaagtc gacgcgggag ttccaggtga gccagctggc tttcccgaag
     1501 gaaccgacgc tatcgaacta cgtcgaggcc tggaagatgg gcgacttcgg cctcttgttc
     1561 ccgaactcgc tgatctatac gatcggcgcg acggtcggca tcgtgttcgt gtcgctactc
     1621 gccggcttcg ctttcgccaa gatcgcctcg cgcgccacga agcctatcta caacggcttc
     1681 gtgatcggta ttctgatcac ggcgcagtcg ttgatgatac ccttgttcct cgaggtaaac
     1741 ctgctgggca tctacaacac gcgcttcgcg gtactgctcg tctatatcgg caccggcctg
     1801 ccgatcggcg tctacctggc caccgagtac atcaaggcca tcccgagcgc gctgatcgag
     1861 tcggcgcgca tcgacggcgc cagtttctgg cgcatcttcg cgaagatcat cgtgccgatg
     1921 tcgatgccgg tggccgtcac gctgtccatc ctgaacatct cgaacatctg gaacgaattc
     1981 gccctcatca acatcctggt ctcgaggacc gagctgaagt cgttgccgct cggcatctac
     2041 aagttctcgg gctcgctgtc gaccgactac ggcaaacagt tcgccgccct gaccataggc
     2101 atggtgccga tgttgctgtt ctatatcgtg ttccgcaaac agattacccg aggcgtcgcg
     2161 gcaggcgcag tcaagggcta gaggatgagg agtacctaca tgacgaacga ccgaacggcc
     2221 caaggacgcg ggtttcccga ggatttcctg tggggttgcg ccacctcgag ctaccagata
     2281 gagggcgggg ctaaggacgg cgggcgaggc cagtcgatct gggacgcctt ctgccgcgag
     2341 ccggggcgcg tggcgctcgg gcagtccggc gaggtcgcct gcgactcgta ccggcgttgg
     2401 cccgaggacg tgcggctcct gtccgggctg ggcgtcggcg cgtatcgctt ctcgatcgcg
     2461 tggccacggg tgcaaccgga cggctccggg gcggttaacc cggccggcct ggactactac
     2521 tcggccctcg tcgacgcgct cctggccgag ggcatcgagt cctgggtcac gttgtaccac
     2581 tgggacctgc cgctggcctt gggcgagcgt gggggctgga ccgatcgcga caccgcgtac
     2641 cgcttcgccg actatgctgg gctcgtgtac gagcgcctcg gcgacagggt gcgccactgg
     2701 accacgctga acgagccgtg gtgttcggcc ttcctcggct acgagcacgg cgagcacgcg
     2761 cccggcctgc gggacaggcg cgcggccctc tcggctacgc atcacctgct cttggcccac
     2821 ggcctcgcgg tcgaggcata ccgcgccacc ggccgtcggg gcgagatagg ccttgtgatc
     2881 aacccggcga cgccgcgacc cgcgacccgc cgcgaggcgg acgtgcaggc ggccggccgg
     2941 gcctcggtgg agcgtaccgg actttggctt gaccccgtgt tcggaagggg ctaccccgag
     3001 gagtacctga aggccagggg cgctacgatg ccgacgcggc ccggcgatct cgagcggata
     3061 gcggcggcca tagatttcgt cggcgtcaac tactacaacg aggacgccgt cgtcgccgcc
     3121 ccgcccacgg ccgagcgccc cgacggctac gatatggcgc cgacggcccg cgagcggacc
     3181 gagatgggct gggacgtggc gccccagggg ctcaggcgcg tgctcggctt catagccgat
     3241 cgctggcccg tgcgcgccct ctacgtgacc gagaatggcg ccgcctaccc ggacgcggcg
     3301 atccgggacg gccgcgcgca tgacgccgac cgtatcgcct attaccgcgg ccatatcgcc
     3361 gcctgcgccg actccatcgg cgacggcgtg ccgctccggg gttacttcgc ctggagcctg
     3421 ctcgataact tcgagtggtc gcacggctac ggtaagcgct tcggtctcgt gtcagtggac
     3481 ccgatgacga tggcacggcg acccaaggat agctattatt tctaccgtga cgtcatagcc
     3541 ggtttcctgg agtagcgctc tcgttcgcat cgccagccgg cgtgtgctat actatcgtca
     3601 tccgataacg ttggtggcat agcggtgaga ttgaagagct ctaccattag cctctgcagg
     3661 aagatactct acgaatcgct gacgacggac gccatggtcc gtttcgtcag gctcgtggag
     3721 cccgaatacg acctgcatga acgctcgggc attccggtca caatccccat cacgaaccag
     3781 atggcggcgg agcgggtcgt ctccgatatc ctcgccgatg gccggttcat cgatttcgtc
     3841 gaggtcctcg tcaagatcga cgccgagggc tatatggggc gatcgtaccc gatcaagggc
     3901 ctggggcaga tcgtcaagat cctcgaacag gacggcttcg tgctggaccg gtccacgggg
     3961 cagtttttcg agaacaccaa tgagcgcgcc agccccgatt ggggccgact ccacgagggc
     4021 gacgaacggc agacctcgat actcaggctc gacgttgtcg acaattcggc cctggtgcag
     4081 cgcaaccggc cggaatgcgt cgatagggcc ttcgtcgcgc tacgctccat cgtcagccgc
     4141 gccgtcgtct cgcgattcgg gcgcgtatgg tcctgggagg gagacggagc gctcgccgcc
     4201 ttcatgttag gccggaagga acgatcggcc gtgctggcgg gcatggagat cctgaacgag
     4261 ctgttcttct tcaaccgctc aggcaatccg ctcgactcgc cgctacgggt caggatagcc
     4321 gcccattccg gcctcgttcg ctacagcgat agtctcatgg aactactcaa gaacgacgcg
     4381 gtcaaggacg cgatacagat cgaagccagc gcgacgccgg ccgatacctt cagcgcctcg
     4441 tccaacctgt tcctatccat cgatcgcacc atccaggatc gcttcgggcc cgataggcga
     4501 tcccccatcg gggacatccg ccattactcc gtgaacctgg agaaggcatg agcgtctacc
     4561 tattctcgat ggacgcctcg cccgccctga gcttcgcgga cgatcgctcg gccaaggccg
     4621 tcgccgtcct cggcctggaa gcgctcaaga gccatcggcc cgagcggcgt gacctgagct
     4681 acatcgacct gcgcgggctc gacgtagaag ccgggcgtaa ggccgtacag accctccggc
     4741 ggcgatgctc gggttcctcc tggggcgtct tcgatcctga agggaccgtc gccgatcctg
     4801 cggccctgtt cttcgccgga gcctccgact atataggacc ggccgtcatc cgggccggcg
     4861 tcgacgcggc caggttcaag gcggcgctgg cctcgacccg gcgccgcgat tccacggccg
     4921 aaggcgaagc tgaggccggt tcgctcgccg cggccggttg cgccgatggc gctccggagg
     4981 gcggcttcgg cggatggaag gccatacggc ctggctcgtc gtacccgttc tgcttcgtct
     5041 acgcgaccgc atcgtcgcag gccaacctga agaccagcct cggggaagcc ggttacgtgg
     5101 ctttccgaga caggctccgg cacatcatgc agcagggcct ggccgaggcc gacgcgctcc
     5161 tatggatgga aaccgactcg agcgtgctct acctcgtgcc gccatatgcg gcgaacgcct
     5221 cggcggcgat cgagttctgt ctgcggaccc tgctcggagc tccgctgatc ggctacgaac
     5281 gactatgcct gccgttcccg ctcgacttta ccttcgctat ccattcgggg actaccgaat
     5341 tcgagccgcc cggcaagacc ggcaccatcg tctcggaggc cgtcaatttc agctaccacc
     5401 taggcgtcaa acgggccgaa cccggtcgag tgactatatc ggccgacgct gccggcttgg
     5461 ccgtgcccgg tcgattctca gatctcttcg tgccggccgg ctccttcgag gggcgatcca
     5521 tcctccattc caggcgcttc tgggcctgat cttcgccgcg gccgcctata gcccgattgc
     5581 gctcctcgtc gaatttctga tagtttctac gcatggtaac gatagagaag gtcgaagccg
     5641 gagcggccgc ggccagggcc atgccgcgcc tgccgcgccg gatcaaggac gacgcgctgc
     5701 ggctcctggc gctcaggctg cgggaacggg ctcccgaact gttcgaggcg aacgaggccg
     5761 acgtcgcggc ggcgagagcc tcgggcgcct cggaggccat ccttacgagg ctcgtctacg
     5821 acggaaagaa actcgaggag tcgatagcga tgatcgacgc cgtctcgcgg ctaccggatc
     5881 cgagcggtcg aacgctcgag tcgaggcggc tcgacgacgg cctcgtcctc agacgggtct
     5941 cctgcccgat cggcctcatc gccctgatct tcgagagccg cccggacgcc ctcgttcaaa
     6001 tggcggcgct ggcggtcaag agcggcgacg cgatcatcgt caagggcggt cgcgaggccg
     6061 agcgcacgaa ccgcgccctc gcggctatcg tcgcggccgc cggcgtagac gcgggcctgc
     6121 cctcgggctg gctagtccag ctggagacac gaagcgaggt cgcccggctc ctggccttgg
     6181 acggtctcgt cgacctggtc ataccccgcg gctccaacga gttcgtcagg catatcatgg
     6241 agacgagtag gatcccggtg ctcggccacg caggcggcgt ctgccacgtc tacgtccacg
     6301 gcgacgcgga tatagccatg gccgttgcga tggtcgtcga tagcaaggcc caatacccgg
     6361 cggcctgtaa cgccgtcgag gtcctgctcg tcgacgaggc agcggcgcgg tctgcgctac
     6421 cggagcttgt cggagccctg gaaagggccg acgtcgcgct cgagctgtgc ccgaggagca
     6481 tggccgtcgt cggcccgggt ccgaccaggg cgatcaagga cgatgacgac tggtcggtcg
     6541 agtacctgtc actccgtatg gcggtgcgcg tcgtcggctc gctcgatgaa gcgatagccc
     6601 atatcaaccg ctacggctcg ggccataccg acgccatcgt ctgttcgtca ggcgaggcgg
     6661 cggcgcgctt catggccgag gtcgactcga cgtcggtcta ccgcaacgcc agcacccgct
     6721 tcgccgacgg ctaccgctac gggctcgggg ccgaggtcgg gatctccacc ggtaagctac
     6781 acgcacgggg gccgatgggg ctcgagggcc tgcagaccta taagtggcta ctcgagggcg
     6841 aggggcatat agtctcggag tactcgtccg gcgccaggcg ctttatccac gaggacctgg
     6901 cggtcgggaa aggcgggatc catggctaag gctaactcgt tcctcgatgc gaggctagcc
     6961 cacgaggcga gggcggccct ggcctcggct aaacgcatcg tcgtcaagat cggcaccgcg
     7021 acgataacca ggcagaaggg gcaggcgggc aggatatcag atggccccga tccgggaggc
     7081 tccggtatcg acgtcgaatt cctgcaccgc gtcgccgcgg agctggccga cctggcgcgc
     7141 gatggcaggc agcccgtgct cgtgacctct ggcgcggtag gcatgggcgc gagggcgctc
     7201 ggcctcgaga agaagcctaa ggacaccgcc tcgaagcagg cctgcgcggc gatcgggcag
     7261 ccgcttctga tggaggagta ccggagggcg ttcgacgtat tcggcctgac cgcggcgcag
     7321 ctcctggtga cccgcgaggc ttgggaggat cgagaagcct acctgaacct gcgctcgaca
     7381 gtcgaggcgc tgctcgagcg cagggcggtg ccggtgttca acgagaacga ctcggtttcc
     7441 accgccgaga tcgcctttgg cgataacgac cgcctgtccg cgtacgtcgc cagcaagatc
     7501 gacgccgacc tgctcgtcat cctgtcggat gtggacgcct tgtacgacgc cgacccgcga
     7561 accgaccccg gggccaggcc gatacccttc gtgcgcgaga tcgaccccgc catgatgtca
     7621 gccgccggcg gcaagggtag cgagttctct accggcggca tgaggaccaa gct
//
LOCUS       DAOPHV010000050         7635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_12256_length_7635_cov_4.004354, whole genome shotgun sequence.
ACCESSION   DAOPHV010000050 DAOPHV010000000
VERSION     DAOPHV010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12256_length_7635_cov_4.004354"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLQ29_02335"
     CDS             complement(<1..299)
                     /locus_tag="PLQ29_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPG85511.1"
                     /translation="MKQDIVELLYRYAELIDAGDFDGVGVLLGRGSFMGVTGAEAIAG
                     LFAASTRRFPEHGGTPRTRHLVLNPIVDIDGDTARARSTFCVLQQTDTVALQPIVV"
     gene            complement(296..1411)
                     /locus_tag="PLQ29_02340"
     CDS             complement(296..1411)
                     /locus_tag="PLQ29_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPG85512.1"
                     /translation="MKRLVAVAAALLVAIAVPAFSDDTPIPGLHRYRLDNGLELFVLE
                     NHAVPLTRVQITFRCGSITQGPETAGLFHLYEHMLFKGNSKYRTETEFSAAMTDLGVA
                     EWNGGTSTEYVTYYFTVPSDKTEAGLEFWSYAMREPLFDPAELSVEQDVVSNEINGFL
                     GDPDRVYSAAMDKALFGAYPWRRDIGGNEAIIRSATVDTLRGIQSTYYVPNNAALFVG
                     GDVDPEQVYAMAKTWYGAWKRGADPWKKPTPAHPMPGVTEPVYLAYPDESLPAGIAYV
                     EMRFRGPDVITEPKPTFAADVWGYLIQAPEGRFKTGVFDSVPALYNKDYLGGYYYTQR
                     DGGQLSFYTYCFTDTSLPIADRAKALFRDAVVKGQVA"
     gene            1498..2367
                     /locus_tag="PLQ29_02345"
     CDS             1498..2367
                     /locus_tag="PLQ29_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HPG85513.1"
                     /translation="MYFPFRPALGRAASALYRSARGGALWRMVVPGAVAESTLAPLAQ
                     LSQLSAPSPGAVAEPRVSRPPLSLKRAALGVARALALFHVWYIGSTMLIIACFSSVDP
                     AATTLALYRKYVDRWDVATPVAVELRRVPLVMRRMIVSAEDGKFWKHRGVDLEAVARA
                     YRVNQAAGEIRYGASTLTMQTARTLFLLPTRSYLRKYLEVLVALEMELILSKERIFEL
                     YLSWAEWGKGVFGVEAASKRYYQTRADKLTPTQAARLVTVLPSPILNTPATAMKNPRL
                     AARYSFIADRYLR"
     gene            complement(2355..2885)
                     /locus_tag="PLQ29_02350"
     CDS             complement(2355..2885)
                     /locus_tag="PLQ29_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HPG85514.1"
                     /translation="MTLWADADSLPREVKELIGRRAGSSTGDFPIRAVFVANRPLPLP
                     PGKNLQAVIVGKAAAQAGGLSTQPERDTLERDTADDYIIASAAPGDILVTRDIPLAAR
                     ALERGLVALNDRGVIWTADSARERASVRDHMAALRELGVAPPLPKGRLFDRKDLKAFA
                     DALDKAIRAAMNAQRR"
     gene            complement(2882..4105)
                     /gene="manA"
                     /locus_tag="PLQ29_02355"
     CDS             complement(2882..4105)
                     /gene="manA"
                     /locus_tag="PLQ29_02355"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HPG85515.1"
                     /translation="MAIYVLENTVQHYAWGSNDALPALLGKENPSGEPWAELWMGAHP
                     RAPSVAVDPSTGARKPLDALIAAAPGATIGADVARRFGGELPFLFKLLSAARPLSVQA
                     HPSKRKAEHGFAREESKGIPIDAAERNYKDPNHKPETVVALTSFEGLCGFRLIEAIVD
                     ATRLLAPDDWERLIGRLAVDPCKLELSSFIYMIMSIDGEHKARRLSYARSRSERIVAD
                     EPRGSGRSRLFSWVLRLMDEYPNDIGALAPLALNLFELSPGEAINLPPGEPHAYLRGT
                     AAEIMANSDNVLRGGLTSKHVDIPEFISTLGFDSAIIEPLEPTLGDDGFYAYPCGVPD
                     YSLSRAEVSGTLTFGGRPETPEILLCVDGEVSLEAGSGASIELPRGASAFVCADERAY
                     AVSGSGAIYRAGVPV"
     gene            complement(4130..6565)
                     /locus_tag="PLQ29_02360"
     CDS             complement(4130..6565)
                     /locus_tag="PLQ29_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HPG85516.1"
                     /translation="MLAIERFTEEAAAELRDAIADAGFNEVFAAGKLDGRGLVAEIIV
                     GARGGQSSVPALAGFLDRGDVLIHNHPSGFLEPSDADLAVASRAGENGVGCYIVDGDV
                     REVYVVAEPVRARALVAIDSGELAGVLDDGGKLASRLPGYEARLSQLELVKAIADAFN
                     GGYVLAAEAGTGVGKSFAYLLPALAWAARNQERVVVSTATINLQRQLVDKDIPIVSSI
                     FKKKLKAVLVKGRGNYLCRTRLAEAIDEEGLFAGDDHPLRRIAAWAESSATGDRSDLS
                     FWPEDALWSRIRSESDECLNMRCPFRESCFVLRMKRDAADADLIVANHHILFSDLASR
                     AAGSGYESTAVLPPYGSIIFDEAHAIEASATSFFSDEITKFSTLRQVGKLYRERRGRK
                     FGIVVKLQNLKGLPSGLLKRFPDASEALVAAAEAVDARALALLSDAPNLRLTVRSKEL
                     EEELLKPLGELERALLSMAELIKDALAGVPDESSEDQSVREAELALVRLSELAAVCAR
                     YRSFDEEPNEIFWLERRKTSQGEGFAQLMITPLDISFMMNEAVFDQFRTVACLSATLS
                     IGGSFGFWKNRVGLSASGARVECREYPSPFPFERNALLAATSDAPDPSSPLWQAWVND
                     AVYELIAASGGRALVLFTSYTALRAAWDAVKPRLDALGISAFRQGDDERSRLLGSFVA
                     DVSSVLFATDSFWEGIDAPGETLELVIITKLPFRVPTDPVQKARSEDIERRGGNSFMD
                     LSLPEAVIRFKQGFGRLIRHSNDRGAVVVLDVRLLTKRYGRVFVSSLPRTRTRFGPLA
                     EVAGEVRSFIGRP"
     gene            complement(6577..>7635)
                     /locus_tag="PLQ29_02365"
     CDS             complement(6577..>7635)
                     /locus_tag="PLQ29_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85517.1"
                     /translation="RYRATRYLAPRFWFPTAILATGGLESAGLAAVLGDPAERLEASV
                     SAAWDTTARAVDLELDVTFSRFAWPIRLLARDSFTGTVPTDADSIVARSSEAFAGTAG
                     SLYPFSGGALSWNVSGGFGANAKSAPGASPYAVWSVASAALESTIAWSDVSAPLGDPE
                     ARSGYVISLFTRLDAALYPTAALPTAGLEATAEAFVGAGALAVSAHGALAVTDGIAYG
                     PDGRAYAGGWTLGASYPSPDDFSGSGYGAWYASLEASLRLLKAEIQRGAGSFYANRLS
                     LRSGGRAYAIAGQDGTARDWGWSAFARASLTWTPAIGAYAALHPVSSLEFWCRPDLAD
                     DGDGVSPHGLTLSLVASY"
BASE COUNT         1218 a         2473 c         2674 g         1270 t
ORIGIN      
        1 acgacgatgg gttgcagtgc gacggtgtcg gtctgctgga gcacgcagaa cgtcgagcgc
       61 gcccgcgcgg tgtccccgtc gatgtcgacg atcgggttga gcaccagatg ccgggtgcgt
      121 ggcgttccgc cgtgctcggg aaagcgacgg gtggacgccg cgaacagtcc ggcgatcgcc
      181 tcggcgccgg tcacccccat gaacgagccg cgccccagca gcaccccgac accgtcgaag
      241 tcccccgcat cgatcagttc ggcgtagcgg tacagcagct ccacaatgtc ctgtttcatg
      301 cgacctggcc cttgaccacc gcgtcgcgga acagggcctt ggctcgatcg gctatgggta
      361 ggctcgtgtc ggtgaagcaa taggtataga aggatagctg gccgccgtct cgctgggtgt
      421 agtagtagcc gccgaggtaa tccttgttgt atagggcagg cacgctatcg aagacgccgg
      481 tcttgaagcg gccttcggga gcctggatca ggtaacccca gacgtcggcg gcgaaggtag
      541 gcttgggctc ggtgatcacg tccggaccgc ggaagcgcat ctcgacatag gctatgccgg
      601 ctggtagcga ctcgtcgggg taggcgaggt agaccggctc ggtcactccc ggcataggat
      661 gggccggcgt cggcttcttc caagggtcag ctccccgctt ccacgccccg taccacgtct
      721 tggccatcgc gtagacctgc tcgggatcga cgtcgccgcc gacgaacagg gcggcgttgt
      781 tcggcacgta gtaggtcgac tggatgccac ggagcgtatc gacggtcgcc gagcggatga
      841 tggcctcgtt gccgccgatg tcccgtctcc aggggtaggc cccgaagaga gccttgtcca
      901 tggccgcgga gtagacgcgg tccgggtctc ccaggaagcc gttgatctcg ttggatacca
      961 cgtcctgctc gacggatagc tccgccgggt cgaatagggg ctcgcgcatc gcgtaggacc
     1021 agaactcgag gccggcctcg gtcttgtcgg acgggacggt gaagtagtag gtgacgtact
     1081 cggtcgaggt gccgccgttc cactcggcga cgccgaggtc ggtcatggcg gccgagaact
     1141 cggtctccgt cctgtacttc gagtttccct tgaagagcat gtgctcgtaa aggtggaaca
     1201 ggccggcggt ctcgggaccc tgggtgatgg agccgcagcg gaaggtgatc tgcaccctcg
     1261 tcagcggcac ggcgtggttt tccaacacga acagttccag gccattgtcg agcctgtagc
     1321 gatggagacc cggaatcggc gtgtcgtcgg agaacgccgg cacggcgatc gcgacgagca
     1381 gcgccgccgc taccgcaact agacgtttca tatgattgcc tcctcgatag gatgctacag
     1441 ggtaggaggg ggtctccctt gtgtcaagcc tgagtactcg ataggatgtc gcccgtgatg
     1501 tatttccctt tccgccccgc gctcggccgc gcggcctcgg cgctgtaccg ttcggctcga
     1561 ggcggcgccc tatggcgcat ggtagtaccg ggcgctgtgg ccgagtcgac gcttgccccg
     1621 cttgcccagc tttcccagct atccgcgccg tccccggggg cggtcgccga gccccgagtc
     1681 tctaggccgc cgttatccct caagcgcgca gcgctcggcg tcgcccgcgc cctggcgctg
     1741 ttccatgtct ggtacatcgg ctcgaccatg ctgatcatcg cctgcttctc gtccgtcgat
     1801 ccggcggcca cgaccctggc cctgtaccgc aagtatgtag accgttggga cgtggctacg
     1861 ccggttgcgg tagagctacg gcgcgtgccg ctggtgatga ggcggatgat agtctcggcc
     1921 gaggacggta aattctggaa gcaccgcggc gtcgacctcg aggccgtcgc cagggcctac
     1981 cgcgtcaacc aggcggcggg cgagatacgc tacggcgcct cgacgctgac gatgcagacg
     2041 gccaggaccc tgttcctgct accgacgcgt agctacctgc gcaagtacct cgaggtgtta
     2101 gtcgccctgg agatggagct tatcctgtcc aaggagcgca tcttcgagct gtacctgtcc
     2161 tgggccgagt ggggcaaggg tgtgttcggc gtcgaggccg cgtccaagcg ctattatcag
     2221 acgcgggcgg acaagctgac gccgacgcag gccgccaggc tcgtgacggt gctgccgtcg
     2281 cccatcctca atacgcccgc gaccgcgatg aagaatcccc gcctcgcggc acgctattct
     2341 ttcatagccg atcgctatct tcgctgagcg ttcatcgcgg cccgtatcgc cttgtcgagc
     2401 gcgtcggcga aggccttcag gtccttgcgg tcgaatagcc ggcccttggg caggggaggg
     2461 gctacgccca gctcgcgtag cgcggccatg tggtctcgca cggaggcccg ctccctggcc
     2521 gagtccgcag tccagatgac gccgcgatcg ttcaatgcga ccaacccgcg ctccagcgcc
     2581 cgggcggcca gcgggatatc ccgcgtcacc aggatgtctc ccggcgcggc cgacgcgatg
     2641 atgtagtcgt cggcggtatc gcgctcgagg gtatcgcgct cgggctgggt cgacaagccg
     2701 cccgcctggg cagcggcctt gccgacgatg acggcttgca ggttcttgcc cggggggagc
     2761 ggcaatggac ggttggcgac gaagacggcg cggatcggga agtccccggt cgaagacccg
     2821 gcccgcctgc cgatgagctc cttgacctcc cgcggtagcg agtcggcgtc ggcccagaga
     2881 gtcatacggg gacgcccgcc ctatagatgg ccccggagcc gctcaccgcg taggcccgtt
     2941 cgtcggcgca gacgaaggcg gacgcgccgc gcggtagctc gatggatgcg ccggacccgg
     3001 cctcgaggct gacttcccca tcgacgcaaa gcagtatctc cggcgtttcc ggccttccgc
     3061 caaacgtaag ggtcccgctg acctcggccc gggacaggga gtagtccggt accccgcacg
     3121 gataggcgta gaagccgtcg tcgccgagcg tcggctccaa aggctcgatg atggcgctgt
     3181 cgaagccgag agtcgatatg aactcgggaa tgtccacgtg cttgctcgtc agcccgccgc
     3241 ggagcacgtt gtcggagttg gccatgatct cggcggccgt cccgcgaagg taggcgtgcg
     3301 gctcgccggg cggcaggttg atggcttccc ctgggctcag ctcgaacagg ttgagcgcga
     3361 gcggcgccag ggccccgatg tcgttggggt attcgtccat cagcctgagt acccacgaga
     3421 aaagccttga acggccggag cctctcggct cgtcggccac gatgcgctcg ctcctcgacc
     3481 tggcgtagct gagccgcctg gccttatgct ccccgtcaat cgacatgatc atgtagatga
     3541 aggacgatag ctcaagcttg cagggatcga cggcgaggcg gccgatcagg cgctcccagt
     3601 cgtccggcgc taacagccgc gtagcatcga ctatggcttc gatcagcctg aaaccgcaga
     3661 gcccctcgaa cgaggtcagg gccacgacgg tctccggctt atggttcggg tccttgtagt
     3721 tgcgttcggc cgcgtctatg ggtatgccct tcgactcctc gcgggcaaag ccatgttccg
     3781 ccttgcgctt cgacggatga gcctgtactg atagggggcg ggccgccgac agcagcttga
     3841 acaggaaggg tagctcgccg ccgaaacggc gggccacgtc cgcccctatc gtcgcgccgg
     3901 gcgcggcggc tatgagcgcg tcgagcggct tcctggcgcc ggtcgatgga tcgacggcga
     3961 cggacggggc gcgtggatgg gctcccatcc atagctcggc ccatggctcg ccgctcgggt
     4021 tctccttgcc cagcagcgcc ggcagggcgt cgtttgagcc ccaggcgtag tgttgtacgg
     4081 tgttttcgag tacgtagatg gccatggggt attgtacccc ggctgtcgct cacggtctac
     4141 cgatgaacga acggacctcc cccgcgacct cggcgagcgg tccgaagcgg gtacgggtac
     4201 ggggtagcga cgatacgaag acgcggccgt agcgcttggt caggagccgc acgtcgagca
     4261 cgacgacggc gccgcggtcg ttcgagtggc ggatcagccg gccgaagccc tgcttgaagc
     4321 gtatgacggc ctccggtagc gataggtcca tgaaggagtt gccgccccgc cgctcgatat
     4381 cctcggatcg ggccttctgg accgggtcgg tcggcaccct gaacggcagc ttggtgatga
     4441 tgacgagctc gagcgtctcg cccggcgcgt ctatgccctc ccagaacgag tcggtcgcga
     4501 acaggacgct cgagacgtcg gcgacgaagg agccgagcag cctggagcgc tcgtcgtcgc
     4561 cctgcctgaa ggcggagatg ccgagcgcgt cgagccgcgg cttcaccgcg tcccaggccg
     4621 cgcgcagggc cgtatacgag gtgaacagca cgagggcgcg gccgcccgag gccgcgatca
     4681 gctcgtagac ggcgtcgttg acccaagctt gccagagggg cgacgatggg tccggggcgt
     4741 cgctggtcgc ggcgagcagg gcattgcgct cgaaggggaa gggcgagggg tactcgcgac
     4801 actcgactct ggcgccggaa gccgataatc cgacccggtt cttccagaat ccgaacgagc
     4861 cgccgatgga aagcgtcgcc gacaggcagg cgacggtgcg gaactggtcg aacaccgcct
     4921 cgttcatcat gaaggaaatg tcgagcgggg tgatcatcag ctgggcgaag ccctcgccct
     4981 ggctggtctt ccgcctctcg agccagaaga tctcgttcgg ttcctcgtcg aagcttcggt
     5041 agcgcgcgca gacggccgcc agctccgaca gcctgaccag ggccagctcg gcttcgcgta
     5101 cggactggtc ctcggacgac tcgtccggta ccccggcgag cgcgtccttg atcagctcgg
     5161 ccatcgacag gagcgccctc tcgagctcgc cgagcggctt gagcagctcc tcctcgagct
     5221 ccttcgagcg tacggtcagg cgcaggttcg gggcgtccga tagcagggcg agggctcgag
     5281 cgtcgacggc ctcggccgcg gcgaccaggg cttccgacgc gtccgggaag cgcttcaaca
     5341 gcccggatgg caggccttta aggttctgga gcttgacgac gatgccgaat ttcctaccgc
     5401 gccgctcgcg gtacagcttg ccgacctggc ggagcgtgga gaatttcgtt atttcgtcgg
     5461 agaagaagct cgtggcgctg gcctctatcg catgggcctc gtcaaagatg atgcttccgt
     5521 agggcggtaa caccgcggtc gactcgtagc ccgagccggc ggcgcgggag gctaggtcgc
     5581 tgaataggat gtggtgattg gctacgatca ggtcggcgtc cgcggcgtcg cgcttcatcc
     5641 tgagcacgaa gcagctctcc cggaaggggc aacgcatatt gaggcactcg tcggattccg
     5701 agcgtatcct cgaccagagc gcgtcctcgg gccagaacga caggtcggag cggtcgcccg
     5761 tggcgctgga ctcggcccag gcggctatcc ggcggagcgg gtggtcgtct ccggcgaaca
     5821 ggccctcctc gtctatggcc tcggcgagcc tggtccggca gaggtagttg ccgcgaccct
     5881 tgaccagtac ggccttgagc ttcttcttga agatcgacga tacgatcggt atgtccttgt
     5941 cgacgagctg gcgctgcagg ttgatcgtgg ccgtcgagac gaccacgcgc tcctggttgc
     6001 gggcggccca ggcgagggcg ggcaggaggt aggcgaacga cttgccgacg ccggtgcccg
     6061 cctccgcggc cagtacgtag ccgccgttga aggcgtcggc tatcgccttg accagctcga
     6121 gctggctgag cctggcctcg tagccgggta gcctcgaggc cagcttgccg ccgtcgtcca
     6181 gcacgccggc tagctcgccc gagtctatgg cgacgagggc ccgggctcgt accggctcgg
     6241 cgaccacgta gacttcccgt acgtcgccgt cgacgatgta gcagccgacg ccgttctccc
     6301 cggcccgcga ggctacggcc aggtcggcgt cgctcggctc gaggaacccg gacgggtggt
     6361 tgtgtatcag cacgtcgccg cggtcgagga aaccggccag ggccggcacc gacgactgtc
     6421 cgccgcgagc tccgacgatg atctcggcca cgaggccgcg cccgtccaac ttgccagccg
     6481 cgaagacctc gttgaagccg gcgtcggcta tggcgtcgcg cagctcggcc gccgcttcct
     6541 cggtgaagcg ctcgatcgct agcatcggcc cggtcctcag tacgaggcga cgagactcaa
     6601 ggtcaggccg tggggggaca caccgtcgcc gtcgtccgct aggtccggcc tgcaccagaa
     6661 ttcgaggctc gataccgggt gcagggcggc gtaggcgccg atagccggag tccaggtcag
     6721 gctcgccctg gcgaaggcgg accagcccca gtcccgcgcc gttccatcct ggccggcgat
     6781 cgcgtaggca cggccgccgg accgaagcga gaggcggttc gcgtagaagg aacctgcccc
     6841 gcgctggatc tccgccttga gcaacctgag gctggcctcg agcgaggcgt accaggcgcc
     6901 gtagccgctc cccgagaagt cgtccggtga cgggtacgag gcgccgaggg tccagccgcc
     6961 cgcataggcc cggccgtcgg ggccgtaggc tataccgtcg gtgacggcga gcgccccgtg
     7021 ggccgagacg gccagggctc cggcgccgac aaaggcttcc gcggtcgctt cgagcccggc
     7081 ggtcggtagg gcggccgtgg ggtagagggc cgcgtcgagc cgtgtgaaga gcgatatgac
     7141 atagcccgag cgggcttcgg ggtcgccgag cggcgcgctg acgtcggacc aggctatcgt
     7201 cgactcgagg gcggcggagg cgacgctcca gacggcgtac ggagaggctc ccggggcgct
     7261 cttggcattg gcgccgaatc cgccggaaac gttccagctc agcgctccgc ccgagaaggg
     7321 atagagcgag ccggcggtac cggcgaaggc ttccgacgag cgcgcgacga tggaatcggc
     7381 gtccgtcggg accgttccgg taaacgagtc ccgcgcgagc agcctgatgg gccaggcgaa
     7441 gcgcgagaag gtgacgtcga gctccaggtc gacggcgcgc gccgtcgtgt cccaggcggc
     7501 cgagaccgac gcctcgagcc gctcggccgg atcgcctagc accgcggcga gacccgccga
     7561 ctcgaggccg ccagtggcga ggatggccgt ggggaaccag aaacggggcg ccaggtagcg
     7621 tgtcgcccgg tatcg
//
LOCUS       DAOPHV010000051         7565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_12418_length_7565_cov_2.938349, whole genome shotgun sequence.
ACCESSION   DAOPHV010000051 DAOPHV010000000
VERSION     DAOPHV010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12418_length_7565_cov_2.938349"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PLQ29_02370"
     CDS             complement(<1..505)
                     /locus_tag="PLQ29_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85518.1"
                     /translation="MNTIPLTLDAYKPTYTVLGGGRGSGCRDGFPVSVLLLNRGPMLY
                     RAEMIQELVRVGFESIVSMEGIGDSPELEGLASRYPQVRFICLHETANLGVRVNIGMR
                     ESCSPFVFVLWNDQRLATSTLSSRFFDKVVDLDAACLVPTLNDASGSPVPSISHPAQS
                     GKAFRIVP"
     gene            complement(480..2015)
                     /locus_tag="PLQ29_02375"
     CDS             complement(480..2015)
                     /locus_tag="PLQ29_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HPG85519.1"
                     /translation="METLAIINAIGLSEHATSPWADGPSALERAVAFAERLAPGRPGA
                     DRHSRILVLASGPLPELGPRSVVRDTWTMRDVLAEADRFASAEPDCDTVIYLRGDEPF
                     LDAGLTGRLLELHGRYRAEYTFADGYPPGFSPELLSVKTLPNLVELASRHEVKPERGD
                     LFALIQNDINSYDIETHLSPRDMRAYRFSPVCDTRRNAQAAERLWKLGGRTAEDAIRI
                     LPGHPELLRTLPAFLWVQVSEGCAQACAYCPYPSMAGDPRGLEGFMPVERFERLMADA
                     EALCDDLVVDLSLWGEPSAHPDFPALARAVLARPRFTLIVETSGVGWERGQAESLAAE
                     AGSRAQWVISLDAVDDEGYLALRGAGKDEAAAFAERLIAASPGNVHVQAVRMKENEAR
                     LEAFYRGWKQRGAKVIVQKYDSFAGSLPDRSVADLSPLERLPCRHLARDMAVLLDGSV
                     PWCKHCLVPGERLRYAGFAGNAFSDGLGSTWAAMGAWYDRHISGELPEPCGRCDEYHT
                     FNA"
     gene            2152..5637
                     /locus_tag="PLQ29_02380"
     CDS             2152..5637
                     /locus_tag="PLQ29_02380"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HPG85520.1"
                     /translation="MKIDRLFTTAGKGPYEGVRWEKRVSEIRNPDGKAVFRMDGVIVP
                     STWSQIATDIIAQKYFRKAGVPDDKAEAWRAWVPSSQQALAGASPVPGSEHDARQVFH
                     RLAYTWTLWAKESGYFDSKADEKAYYDEMCFMLANQMGAPNSPQWFNTGLHAVYGLEG
                     PAQGHYYVDPKSGQVVASTNAYERPQPHACFILDVQDDLVNDGGIMDLVEREARLFKY
                     GSGTGSNFSKIRGAGEPLSGGGFSSGLLSFLKIADRSAAAIKSGGTTRRAAKMVILDA
                     DHPDIEQFVDWKAGEEYKVACLVAGSKALKQYSSAMRDAAARSDVPASDRLSIDANPW
                     LRSAAKEALDAGVPGSYVYQLLRRIADGDPDVDGPVYDASWEGEAYNTVSGQSSNNSV
                     RVTNEFMEAVAKGKDWALTMRRGGKTLRTIKARSLWDRIAKSAWQCADPGLQFHTTIN
                     DWHTCPADGEIRGSNPCSEYMFLDDTACNLASLKLTAFYDERARSFDVDRYRHAVRLW
                     TLTLEISVAMAQFPSKEIARKSYDYRTLGLGYANLGTLLMLMGLPYDSAEGRAVAAAI
                     SAIMTGEAYAHSARMAAAFGPFARFGANADEMLRVMRNHRRAAYGVAAAEYEKLSVAP
                     QGIDPAKAPAALVESARAAWDEALELGRSHGYRNAQTTAIAPTGTIGLLMDCDTTGIE
                     PDFALVKFKKLAGGGYFKIVNNSVEPALRALGYSEEQIGDIRSFMVGRGSLEGAPGVS
                     LEALRAKGLPEEALAKADKALRDAMGLDAAFNPFTLGQEVYAAVGATEALRADPGLDL
                     LAFLGFAQAEIAQAEAWACGSMGIEGAPHVKKSHYAVFDTATPSGREATRSIAWEAHI
                     AMMAATQPFVSGAISKTINMPDSASFDDVKGVYMTAWKSMLKSIALYRDGSKLSQPLS
                     AIAASSNPLAERLVALQSEPAEPVAAPSAGDQAAAEAPSFVSARHSLPNRRDGYTQKA
                     KIGGHSVFVRTGEYDDGRLGEIFLDMHKEGAAFRSLLNSFAIAVSLGLQYGVPLDEYV
                     DAFTFSRFEPNGMVQGHEYIKMATSVLDYVFRDLAISYQDRFDLGQVKPEDLNATNTQ
                     NGKAARKEAERRALETAERAAPAQSAARPKDDSRKARMKGYEGDPCPVCGHLTLVRNG
                     TCLKCETCGSTTGCS"
     gene            5700..6944
                     /locus_tag="PLQ29_02385"
     CDS             5700..6944
                     /locus_tag="PLQ29_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HPG85521.1"
                     /translation="MDRRLRILSETGRLRRVIVHTPGAEIEAMRPSRAEEELYNDIIP
                     LEVVKREHSQLKGFLAKVAEVSELGDLLVDALGSADKRERFAAMLADHYGAAHRLAEL
                     EAMDAGSLAKASIGGLPVRHVRLASYVRGAEFDIPPLPNMYFMRDAAMVYKDKSISSA
                     MRFPVRRAESLINRFVFENHPDFKGGGRLIDGPAMVGDGFSIEGGDFQVVRGDVVVIG
                     VSERTSARAIDAVAEAIVGSYGEPVTLFAVLLPVARATIHLDMAFTVIDRDACLVYAP
                     VMLGHNRARVVRIDAAPGKEPRLSEPESLLAGLTSVGVDLKPVLTGGSDPLMQEREQW
                     LSGANSFSFAPGKIIMYSCNVRTLESLSAAGFEIRSASDFTAGGEDPLSFKRLAVGFD
                     GVELARGGGGARCMTCPVERED"
     gene            complement(7020..7190)
                     /locus_tag="PLQ29_02390"
     CDS             complement(7020..7190)
                     /locus_tag="PLQ29_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPG85522.1"
                     /translation="MAYKITDACVNCGACEPECPVEAISEKNGARAIDAAKCIDCGSC
                     ASVCPTEAIVQG"
     gene            complement(7275..>7565)
                     /locus_tag="PLQ29_02395"
     CDS             complement(7275..>7565)
                     /locus_tag="PLQ29_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85523.1"
                     /translation="STAMEAIFLLKPMVFTEWSTYNDRYIINFALDYNIGMYCPTPFS
                     FIRAMRAVVEDDRLRVCAENLTELHMRPGTDEVAAFIAGQLGHGGSNVQAPG"
BASE COUNT         1369 a         2575 c         2451 g         1170 t
ORIGIN      
        1 agggcactat cctgaaggcc ttgccagact gggcgggatg gctgatcgac ggcacgggcg
       61 agcccgaggc gtcgttgagg gtcggcacga ggcaggccgc gtccaggtcg accaccttgt
      121 cgaagaagcg gctcgacagc gtgctcgtgg ccaggcgctg atcgttccat agcacgaaga
      181 cgaagggcga gcacgattca cgcatgccga tgttcacgcg aacgccgagg ttcgcggtct
      241 cgtgcaggca gatgaagcgg acctggggat agcgcgacgc caagccttct agctcgggag
      301 aatcgccaat accctccatc gacacgatcg actcgaagcc gacgcgcacc agctcctgga
      361 tcatctcggc gcgatatagc atcggcccgc gattcaggag tagcaccgac acgggaaagc
      421 cgtccctgca accggagcct cggccgccgc cgagcacggt gtaggtgggc ttataggcgt
      481 caagcgttaa aggtatggta ttcatcgcac cgtccgcatg gttcaggcaa ctcgccgctt
      541 atatgcctat cgtaccaggc acccatagcc gcccaggtcg agccgaggcc gtcggagaac
      601 gcgttaccgg cgaagcctgc gtatcgtagg cgttcgccag gcacgaggca atgcttgcac
      661 cagggtaccg agccgtcgag cagtacggcc atgtcgcggg ccaggtggcg gcagggaagc
      721 cgctcgagcg gcgataggtc ggcgacgctc cgatcgggga ggcttccggc gaaggagtcg
      781 tacttctgca ctatcacctt ggcccctcgc tgtttccagc cccggtagaa ggcctcgagt
      841 ctggcctcgt tctccttcat gcgcaccgcc tggacgtgga cgttgccggg cgaggctgcg
      901 atcaagcgct cggcgaaagc cgcggcctcg tccttgcccg ctccgcgcaa cgccaggtag
      961 ccctcgtcgt cgaccgcgtc gagggagatg acccactggg cacgggagcc ggcctcggcg
     1021 gccagggatt cagcctggcc ccgctcccag ccgacgcccg aagtctcgac gatcagcgtg
     1081 aagcgcgggc gcgccaagac ggcccgagcc agcgccggga aatccgggtg cgccgagggc
     1141 tcgccccaga gggacagatc gacgaccaga tcatcgcaga gagcctcggc gtcggccatc
     1201 agccgttcga agcgctcgac cggcatgaag ccctcgagcc cccgcgggtc gccggccatc
     1261 gacgggtacg gacagtaggc gcaggcctgg gcgcagccct cgctaacctg aacccagagg
     1321 aaggccggca gggtcctcag gagctccggg tgcccgggca ggatccggat ggcgtcctcg
     1381 gcggtcctgc cgccaagctt ccagaggcgc tcggccgcct gcgcgttccg cctggtgtcg
     1441 cagacgggcg agaagcgata ggcgcgcata tcgcgaggag acaggtgcgt ctcgatatcg
     1501 tacgagttga tgtcgttctg aatcagcgcg aacagatcgc cccgctccgg cttgacctcg
     1561 tggcgcgacg ccagctccac gaggttcggc agggtcttga ccgatagcag ctccggcgag
     1621 aagccgggcg ggtacccatc ggcgaaggtg tactcggcgc ggtagcgccc gtgcagctcg
     1681 agcaggcgcc cggtcagccc cgcgtcgagg aagggctcgt cgcctctcag gtagatcacg
     1741 gtgtcgcagt caggctcggc ggacgcgaag cggtcggcct cggcgagtac gtcgcgcatc
     1801 gtccaggtat cgcggacgac cgaacgcggc ccgagttccg gcaagggacc tgaagccagc
     1861 acgagtatcc tggaatgcct atcggccccg gggcgccccg gagccaggcg ctcggcgaag
     1921 gcaaccgccc gctcgagggc gctcgggcca tcggcccagg gggaggtcgc gtgctcgctc
     1981 aagcctatcg cgttgattat cgctagtgtt tccatagtca tacttagtat cggcctcgat
     2041 ccaactcgcc gttagccgct aatacaggcc tcgacacata cactatatag cagtataata
     2101 tccaggcaat acaataagtt gtatatttat cgatcgcatg gggaggacat catgaagata
     2161 gacagactgt tcacgaccgc cggcaagggc ccgtacgagg gcgtccgatg ggaaaaacga
     2221 gtctccgaga tacggaaccc cgacggcaag gcggtcttcc ggatggacgg cgtcatcgtg
     2281 ccgtcgacct ggagccagat agccaccgac atcatagccc agaaatactt tcgcaaggca
     2341 ggcgtgcccg atgacaaggc cgaggcttgg cgcgcctggg taccgtcgtc gcagcaggcc
     2401 ctggccggcg cctcccccgt gcccgggtcc gagcacgacg cgcgccaggt gttccaccgc
     2461 ctggcctaca cctggacgct ttgggccaag gaatcgggct acttcgattc gaaagccgac
     2521 gagaaggctt actacgacga gatgtgcttc atgctggcca accagatggg cgctccgaac
     2581 agcccgcagt ggttcaacac cggcctgcac gccgtgtacg gcctcgaggg cccggcccag
     2641 ggccactact acgtcgaccc gaagagcggc caggtcgtcg cctcgacgaa cgcctacgag
     2701 cggccccagc ctcacgcctg cttcatcctg gacgtgcagg acgacctggt caacgacggc
     2761 ggcatcatgg atctcgtcga gcgcgaggcg aggctgttca agtatggctc gggtaccggc
     2821 tcgaacttct cgaagattcg cggcgcgggc gagccgctgt cgggcggcgg cttctcgagc
     2881 gggctcctgt ccttcctcaa gatagccgac cgctcggcgg cggctatcaa gtccggcggc
     2941 acgacccgcc gcgccgccaa gatggtcatc ctcgacgccg accatcccga catcgagcag
     3001 ttcgtcgact ggaaggccgg cgaggagtac aaggtcgcct gcctcgtcgc agggagcaag
     3061 gcgctcaagc agtactcgag cgccatgcgc gacgccgcgg ccaggtccga cgtgcccgcg
     3121 tccgaccggc tgtcgataga cgccaacccg tggctgcgct cggccgccaa ggaagccctc
     3181 gacgccggcg taccgggctc ctacgtctac cagctcctca ggcgcatcgc cgacggcgac
     3241 ccggacgtcg acgggccggt ctacgacgcc agctgggagg gcgaggccta caacaccgtc
     3301 tccggccagt cgtcgaacaa ctcggtccgg gtcacgaacg agttcatgga ggccgtggcc
     3361 aagggcaagg actgggcgct gacgatgcgc cgaggcggca agaccctgcg caccatcaag
     3421 gcccgctcgc tctgggaccg catagccaag tccgcgtggc agtgcgccga tccggggctc
     3481 cagttccaca cgaccattaa cgactggcat acctgccccg ccgacggcga gatacggggc
     3541 tcgaacccct gctccgagta catgttcctc gacgacacgg cctgcaacct ggcctcgctc
     3601 aagctgaccg ccttctatga tgagcgggcc cggtccttcg acgtggatcg ctaccgccac
     3661 gccgtcagat tatggaccct gaccctcgag atatccgtag ccatggccca gttcccctcc
     3721 aaggagatag ccagaaagag ctacgactac cgcacgctcg gcctcggcta cgccaacctc
     3781 ggcacgctcc tcatgctgat gggcctgccc tacgactcgg ccgagggacg agcggtcgcc
     3841 gcggctatca gcgccatcat gaccggggag gcctacgccc actccgccag gatggccgcc
     3901 gccttcgggc ccttcgctcg gttcggggcg aacgccgacg agatgctgcg cgtgatgcgc
     3961 aaccaccggc gagccgccta cggcgtggcc gcggccgagt acgagaagct gtcggtggcg
     4021 ccccagggca tcgacccagc caaggcgccg gccgccctcg tcgagtccgc cagggcggcc
     4081 tgggatgagg ccctcgagct gggccggtcc cacggctacc gcaacgccca gaccacggcc
     4141 atcgcgccga ccggcaccat cggcctcctc atggactgcg acaccaccgg catcgagccg
     4201 gacttcgcgc tggtcaagtt caagaagctg gccggcggcg gctacttcaa gatagtcaac
     4261 aattcggtcg agccggccct gcgcgcctta ggctattccg aggagcagat cggcgacatc
     4321 aggtcattca tggtcggccg cggctccctc gaaggcgctc ccggcgtctc gctcgaggcg
     4381 ctacgcgcca agggcctgcc cgaggaagcg ctcgccaagg ccgacaaggc tctccgcgac
     4441 gcgatgggcc tcgacgcggc cttcaacccg ttcacgctcg gccaggaagt ctacgcggcc
     4501 gtcggcgcga ccgaagcgct gcgggccgat cccggcctcg acctcctggc cttcctcggc
     4561 ttcgcgcagg ccgagatagc ccaggccgag gcctgggcct gcggctcgat gggcatcgag
     4621 ggcgcgcccc acgtcaagaa gtcccactac gccgtcttcg ataccgcgac cccgtccggc
     4681 cgcgaggcga cgaggtccat agcctgggaa gcccatatag cgatgatggc cgccacccag
     4741 cccttcgtct cgggcgccat ctccaagacc atcaacatgc ccgactcggc cagcttcgac
     4801 gacgtgaagg gcgtctacat gacggcctgg aagagcatgc tcaagagcat cgcgctctac
     4861 cgcgacggct caaagctgtc ccagcccctt tcggctatcg cggctagctc gaatccgctc
     4921 gccgagcggc tcgtcgccct gcagtccgaa ccggccgagc ccgtcgccgc accgagcgcc
     4981 ggcgaccagg cggccgccga ggcgccgagc ttcgtcagcg ccaggcattc cctgccgaac
     5041 cggcgcgacg gctacaccca gaaggccaag ataggcggcc actcggtgtt cgtacgcacc
     5101 ggcgaatacg acgacggccg cctgggcgag atcttcctcg acatgcacaa ggagggagcc
     5161 gccttcagga gcctcctcaa ctcgttcgcc atcgccgtct cgctcggcct ccagtacggc
     5221 gtgccgctcg atgagtacgt cgacgcgttc accttcagcc gcttcgagcc gaacggcatg
     5281 gtccagggcc acgagtacat caagatggcc acgagcgtgc tcgactacgt gttccgagac
     5341 ctggctatca gctaccagga ccgcttcgac ctcggccagg tcaagcccga ggacctgaac
     5401 gccacgaaca cccagaacgg caaggccgcc aggaaagagg cggaacgccg ggcgctcgag
     5461 actgccgagc gagccgcgcc ggcccagtcc gcagccaggc ccaaggacga ctcccgcaag
     5521 gccaggatga agggctacga gggcgacccc tgtcccgtct gcggccacct gaccctggtc
     5581 cgcaacggca cctgcctgaa atgcgagacg tgcgggagca ccacaggctg cagctgagcc
     5641 catcgcgggt tgcggattcg aggcatctct tggtatcctg tcgtcaaaag ggaggcttta
     5701 tggacaggag gctgaggata ctctcggaga cgggtcgtct ccgtcgagtc atcgtgcaca
     5761 ctcccggagc ggagatcgag gcgatgcgcc cgagccgcgc cgaggaggaa ctctacaacg
     5821 acatcatccc gctcgaggtg gtcaagcgcg agcactcgca gctcaagggc ttcctggcga
     5881 aggtcgccga ggtcagtgag ctgggtgacc tgctcgtcga cgccctcggc tcggctgata
     5941 agcgcgaacg attcgcggcc atgctcgccg atcactacgg cgcggcccac cgactggccg
     6001 agctcgaggc gatggacgcc ggatcgctcg ccaaggcgtc gataggcggc cttccggtca
     6061 ggcacgtcag gctcgcgagc tacgttcgcg gcgccgagtt cgatataccg ccgctaccga
     6121 acatgtactt catgcgcgac gcggccatgg tctacaagga caagtcgatc tcctcggcga
     6181 tgcgcttccc ggtgcgccgc gccgagtcgt tgatcaaccg cttcgtattc gagaaccacc
     6241 ccgatttcaa gggcggcggg cgcctgatag acggaccggc catggtcggg gacggcttct
     6301 cgatcgaggg cggcgacttc caggtcgtcc gaggcgacgt cgtcgtcatc ggcgtatccg
     6361 agcgcaccag cgcccgggcg atcgacgcgg tggccgaggc catcgtcggc tcgtacgggg
     6421 agccggtcac gctgttcgcg gtgctgctgc ccgtcgccag ggcgacgata cacctggata
     6481 tggcctttac cgtaatcgac cgcgacgcct gcctggtcta cgcgccggtg atgctcgggc
     6541 ataaccgggc ccgggtcgtc aggatagacg ccgcgccggg caaggaaccg cgcctctcgg
     6601 agcccgagtc cctgctcgcc ggcctcacgt cggtcggcgt cgacctgaag ccggtcctga
     6661 ccggcggaag cgatccgctc atgcaggaac gcgagcagtg gctatccggg gccaactcgt
     6721 tcagcttcgc cccgggcaag atcatcatgt actcgtgcaa cgtgcgcacc ctcgagtcgc
     6781 tgtcggcggc cggcttcgag atacgctcgg cgtcagattt caccgccggc ggcgaggatc
     6841 cgctgtcctt caagcggctg gccgtcggct tcgacggcgt ggaactggcc cggggcggcg
     6901 gcggcgcccg ctgcatgacc tgcccggtcg agcgggagga ctgaaccccc attcccgcct
     6961 ccgatactaa aaaaagaacg ctccctcgcg ggagcgttct tcgtatcgac gcgctggcgt
     7021 cagccctgga cgatagcctc ggtcgggcag acggaggcgc aggagccgca gtcgatgcac
     7081 ttggcagcgt cgatggcgcg ggcgccgttc ttctcgctga tggcctcgac gggacactcg
     7141 ggctcgcagg cgccgcagtt cacgcaggcg tcggtaatct tgtatgccat ggtacaatct
     7201 ccttagatgg cttgatgtgc ctcgatacta gcatacgact aaaagcctcg caagatcgtc
     7261 agccagttaa acacctagcc tggagcctgg acgttagacc ctccatggcc cagctggccc
     7321 gcgatgaaag cggccacctc gtcggtgcca ggacgcatat ggagctcggt cagattttcg
     7381 gcgcagacgc gtagcctatc gtcctcgacg accgcccgca tcgccctgat gaacgagaac
     7441 ggcgtcggac agtacatgcc tatattgtaa tcgagcgcga agttgatgat gtagcggtcg
     7501 ttgtaggtcg accactcggt gaacaccatc ggcttcagca ggaagatggc ctccatcgcg
     7561 gtcga
//
LOCUS       DAOPHV010000052         7500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_12570_length_7500_cov_4.153660, whole genome shotgun sequence.
ACCESSION   DAOPHV010000052 DAOPHV010000000
VERSION     DAOPHV010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7500
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12570_length_7500_cov_4.153660"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLQ29_02400"
     CDS             complement(<1..232)
                     /locus_tag="PLQ29_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HPG85524.1"
                     /translation="MAAEKRLMKGNEAMGEAAVRAGCDAYFGYPITPQNELTAYMAKH
                     MIDHKRVFIQAESEVSAINMVYGAAATGARAMT"
     gene            complement(245..460)
                     /locus_tag="PLQ29_02405"
     CDS             complement(245..460)
                     /locus_tag="PLQ29_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPG85525.1"
                     /translation="MATKGKPEIDRERCKGCLLCVRACPVKVLAADTEPNSWGYYPST
                     AVAPEKCIACGNCYAVCPDVAISVYKL"
     gene            complement(548..1444)
                     /locus_tag="PLQ29_02410"
     CDS             complement(548..1444)
                     /locus_tag="PLQ29_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HPG85526.1"
                     /translation="MKTIADILAAARAAGRKRIAVAAAQEASALEAALDARAAGIAEP
                     VLIGDLEAIRSIAAELGADLSGLRLIEEKDYARAAAAAVALVRSGEADVLMKGVLDTS
                     ILLKAALNKEVGLNAGRLTSHVAVIESPHYHKLFIVTDAAINIAPDLQGKLDIVANAV
                     AVSRSIGVELPKVAMLAAVEKVNPEKMPCTADAALITQMNRRGQVKGCVVDGPLALDN
                     AISAESARIKKIVSDVAGDADVLVVPDIEAGNVLYKALLDLGGAKGAGIVAGAAKPIV
                     LTSRADSRETKLASIALAALAG"
     gene            complement(1446..2510)
                     /gene="buk"
                     /locus_tag="PLQ29_02415"
     CDS             complement(1446..2510)
                     /gene="buk"
                     /locus_tag="PLQ29_02415"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPG85527.1"
                     /translation="MSQRILVINPGSTSTKIGVFEDGIESFTESVAHSAEEIARYPAI
                     ADQFEFRERTIRAALEKRGFDIRTVACVVGRGGLLRPIPGGVYAVSETMKDDLRSGRY
                     GEHASNLGGLIADALARELGVPAFIADPVVVDELSDLARLYGHRLFRKQSIFHALNQK
                     AVARRWATERGKRYDEVNLIVAHMGGGVSVGLHRKGRVVDVNNALNGEGPFSPERAGT
                     LPAGALAKLCFSGEYTLPQVMKMINGQGGMVSLAATNDMRVLEAKVAEGDAEAKLVYD
                     AFVYNVGKAIGALAAAADGTIDGIVLTGGIAYGQAVRDGLSRMTGWIAPVTAYPGEGE
                     LEALAAAGARGLAGEAMEYR"
     gene            2728..3753
                     /gene="tdh"
                     /locus_tag="PLQ29_02420"
     CDS             2728..3753
                     /gene="tdh"
                     /locus_tag="PLQ29_02420"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HPG85528.1"
                     /translation="MKALVKKSPEVGLWLEDVPMPEVGDNDLLIKVKKTAICGTDVHI
                     YKWDEWSQRTIKTPMTIGHEFVGEIVEMGRAVQGYKVGERVSAEGHIVCGVCRACRAG
                     KRHLCPNTRGIGVNRDGCFAEYVSVPASNAWHVHDSIPDDIAAIFDPYGNATHTALSF
                     DMVGEDVIITGAGPIGCMAAAIARHVGARNVVVTDVNDYRLELAKRLGATRVVNVARD
                     DLRAVMREIDMVGGFDVGLEMSGSPQAFKQMLDNMYNGGRVAMLGLMPNGAGIDWDKV
                     IFKGLFIKGIYGREIFETWYKMQTMVLSGLDIAPVITHRFSFDDFQKGFDAMLSGQSG
                     KVILSLD"
     gene            complement(4038..4424)
                     /locus_tag="PLQ29_02425"
     CDS             complement(4038..4424)
                     /locus_tag="PLQ29_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPG85529.1"
                     /translation="MRFSSERPIFSQIAELLAEDVLSGRLGPGARLPSARELAASLGV
                     NPNTAARALQALADDGVARCERGTGYFVADGGRDVALGGRRRRFLEEELPRLWKGMDE
                     LGLGLDDLAGYWAARQARGDDERIGS"
     gene            complement(4411..5214)
                     /locus_tag="PLQ29_02430"
     CDS             complement(4411..5214)
                     /locus_tag="PLQ29_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85530.1"
                     /translation="MNRDTLSSRFSAGRVMLLARNRAYDDAPGLGIGLAIVAGLNLLS
                     ILIADRAFMNSEGDPSYAGLIIISGLILSSQAFKGMHGRTGADWILLPATPLEKYAAA
                     FVSYLVVFPLVAAAAAMGLSFVLALVERAAGGPGTGIWHPISLAALRSYGEYAVVAVA
                     FLAGSAAFRKRAFLKTAGLSVAFVLLCSLVVYLGLSLVHEATGSRGFSVSYDSGSLTV
                     LGGDGPGALSVERTLRVLVDVVRLALVPAFSIAFGYLRVAEKEARDEVQ"
     gene            complement(5211..6101)
                     /locus_tag="PLQ29_02435"
     CDS             complement(5211..6101)
                     /locus_tag="PLQ29_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG85531.1"
                     /translation="MVRMESMTFGYGKRPLFEDLDLRLEPGAIHGLLGLNGAGKTSLL
                     KIAAGALRPERGSVEVYGRAPGKREAAALADVCFVPEDPCFPALKPAAWLGRYAAFRP
                     AFDHGRFDELFAELGLDADKPISKFSYGQRKKFALAAAVASGSRLLLLDEPTNGLDIP
                     SKAQLRRVLAAASGPDRVVVVSTHQIRDLELLLDPIIVIDRGRVAFTVTMAELSDRLG
                     AARLPSIEGRPVVYAERDAVGWTALLATAGPGEAAPPDLELVFNAAIAAPDRLAAALR
                     GDALPEYRPEAAALAAGGRA"
     gene            6380..>7500
                     /locus_tag="PLQ29_02440"
     CDS             6380..>7500
                     /locus_tag="PLQ29_02440"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HPG85532.1"
                     /translation="MLGLESFRTPSAPKEAAHLVSTLLDIVEEVAPLPAGRRKDIRRD
                     VLELWRELTSEKSTRQPDYIGEPAKLAAYLRYFLPWNVARLVPLLVDLDLGLKPGDAV
                     LDIGSGPLTVPIALWIARPDLRAMELRVECMDRVRRAMEIGLGLLEGLALRSGQALSW
                     KLTIQKESFSITDAAPRRDFALVTSANVFNESFWRIKGSLSERADMLASSLGARAARG
                     GRILIVEPGDPRSGAMLSALRESVILRGGEPLAPCPHRAACPMAGAFLSSAFRDSDED
                     GRDGGERLAKVVTAKGRSKAPWCHFVIDAGAAPERLVAFSETAGLPKDRLIASWLLLR
                     PDAAEKTPSAQAPSRSVRLVSDAFRLAEGGLGRYACTRTG"
BASE COUNT         1268 a         2637 c         2369 g         1226 t
ORIGIN      
        1 tggtcattgc gcgggcgccg gtcgcggcgg ccccgtagac catgttgatc gcggagacct
       61 cgctctcggc ctgtatgaac acgcgtttgt ggtcgatcat gtgcttggcc atgtacgcgg
      121 tcagttcgtt ctgcggggtg atggggtagc cgaagtacgc gtcgcatcca gcgcggacgg
      181 cggcctcgcc catagcctcg ttgcctttca tcaggcgctt ctctgcagcc atgccggacc
      241 ctccttatag cttatagacg ctgatcgcca cgtccgggca gaccgcgtag caattaccgc
      301 aggctatgca cttctcgggc gctacggccg tggacggata gtatccccac gaattgggct
      361 ccgtatcggc cgcgagcacc ttgaccgggc aggcgcggac gcagagcagg cagcccttgc
      421 atcgttcccg atcgatctcg ggttttcctt tagtagccat ggcgactcct tgtcgtactt
      481 cccgtacgcg tagaatggta tcggggaccg cgcggccccc ggcgattgcc aaatgcgttc
      541 ctcgcggcta gccggcgagc gccgccaggg ctatgctcgc cagcttcgtc tcccggctgt
      601 cggcccggct cgtcagcacg atcggcttgg ccgccccggc gacgattccc gcccccttag
      661 cgccgcccag gtccagcaac gccttgtaca gcacgttgcc cgcctcgatg tccggcacga
      721 ccaatacgtc agcgtcgccg gccacgtcgg agacgatctt cttgatgcgg gcgctctcgg
      781 cgctgatcgc gttgtccagc gccagcggcc cgtcgacgac gcagcccttc acctgtccgc
      841 gccggttcat ctgcgtgatc agggcggcgt cggccgtgca cggcattttc tccgggttga
      901 ccttctccac cgccgccagc atggccacct tcggtagctc cacgccgatc gagcgggaca
      961 cggccaccgc gttcgcgacg atgtcgagct taccctgcag gtcgggcgct atattgatcg
     1021 ccgcgtcggt gacgatgaac agcttgtggt aatgcggcga ctcgatcacc gccacgtggc
     1081 tcgtcaggcg gccggcgttc agaccgacct ccttgttgag cgcggccttg agcaggatcg
     1141 acgtatccaa gacgcccttc atgagcacgt cggcctcgcc cgagcgcacg agtgccacgg
     1201 ccgccgccgc cgccctggcg taatccttct cctcgatcag gcgcaggccc gagaggtcgg
     1261 ccccgagctc ggccgcgatg gagcgaatcg cctcgaggtc gcctatcagc acgggctcgg
     1321 caatgcccgc cgccctggcg tcgagcgccg cctcgagcgc cgaggcttcc tgggcggccg
     1381 ccacggctat ccgcttgcgg ccggcggccc gcgccgcggc cagtatatcg gctatcgtct
     1441 tcatgttacc tgtactccat cgcctcgccg gccagccctc gggcgccggc agcggccaag
     1501 gcctcgagct cgccctcgcc cgggtaggcg gtcaccgggg ctatccagcc ggtcatcctc
     1561 gataggccgt cgcgcaccgc ctgaccgtag gctatgccgc cggtcagcac gatgccgtct
     1621 attgttccgt cggccgcggc ggcgagcgcc ccgatagcct tgccgacatt gtagacgaac
     1681 gcgtcgtaga cgagcttggc ttcggcgtcg ccctcggcta ccttggcctc gagcacgcgc
     1741 atgtcgttcg tagcggccag gctgaccatg ccgccctggc cgttgatcat cttcatcacc
     1801 tgcggcaggg tgtactcccc ggagaagcac agcttggcca gggcgccagc cggcagggtg
     1861 cccgcccgct cggggctgaa cggaccttcg ccgttgagcg cgttattcac gtcgacgacg
     1921 cggcccttcc ggtgcaggcc gaccgagacg ccgccgccca tgtgcgcgac gatcaggttc
     1981 acctcgtcgt agcgcttacc gcgctcggta gcccagcggc gggccacggc cttctggttg
     2041 agcgcgtgga agatgctctg cttgcggaac agcctatgcc cgtagagcct ggccaagtct
     2101 gacagctcat ccacgaccac cgggtcggcg atgaacgccg gtacgcccag ctcgcgggcc
     2161 agcgcgtccg cgataagccc gccgaggttc gaggcgtgct cgccgtagcg ccccgaccgc
     2221 agatcgtcct tcatcgtctc ggagacggcg tagacgcctc ccgggatcgg ccgtagcagc
     2281 ccgccgcgtc cgaccacgca cgcgacggtc cgaatatcga agccgcgctt ctcaagggcc
     2341 gcccggatcg tccgctcccg gaattcgaac tggtcagcta tcgcgggata gcgggcaatc
     2401 tcctcggcgc tatgggccac gctctcggtg aacgattcga tgccgtcctc gaaaacgcct
     2461 atcttcgtcg atgtcgatcc tggatttatg acgaggatcc tctgtgacat gcgaactcct
     2521 tgggggtgaa acgagaatag tactaagcgt tgaaacggag tatacgcaat tgagacggta
     2581 tacgcaagta aaaaacatag attgtaacca ttttttaaaa tggcatttca tattacgaaa
     2641 caatggtgac gattattgat agaatcctct tcaccgacgc ctcgcggaac gctatactgc
     2701 gttcgtagtt ctcttttgga ggcaagcatg aaggctctcg tcaagaaatc gcccgaggtc
     2761 gggttatggc tggaagacgt cccgatgccc gaggtaggcg acaacgatct cctcatcaag
     2821 gtcaagaaga ccgccatctg cggcaccgac gtccacatct ataaatggga cgaatggtct
     2881 cagcgcacca tcaagacgcc gatgaccatc ggccacgaat tcgtcggcga gatcgtcgag
     2941 atgggccgcg cggtccaggg ttacaaggtc ggagagcgcg tctcggccga gggccacatc
     3001 gtctgcggcg tctgtcgcgc ctgccgggcc ggcaagcgcc acctctgccc gaacacgcgc
     3061 ggcatcggcg tcaaccgcga cggctgcttc gccgagtacg tctcggttcc cgcctccaac
     3121 gcctggcacg tccacgactc catccccgac gacatagcgg ccatcttcga cccctacggc
     3181 aacgccaccc acaccgccct gtccttcgac atggtcggcg aggacgtgat catcaccgga
     3241 gccggcccga taggctgcat ggccgccgcg atagcgcgcc acgtcggcgc ccgcaacgtg
     3301 gtcgtcaccg acgtcaacga ttaccgcctc gagctggcca agcgactcgg cgccacgagg
     3361 gtcgtcaacg tcgcgcgcga cgacctccgc gccgtgatgc gcgagatcga catggtcggc
     3421 ggcttcgacg tcggcctcga gatgtccggc tcgccccagg ccttcaagca gatgctcgac
     3481 aacatgtaca acggcggacg cgtcgccatg ctcggcctca tgccgaacgg cgccggcatc
     3541 gactgggaca aggtcatctt caagggcctg ttcatcaagg gcatctacgg ccgcgagatc
     3601 ttcgagacct ggtacaagat gcagacgatg gtcctctccg gcctcgacat cgcccccgtc
     3661 atcacccacc gcttctcctt cgacgatttc cagaagggct tcgatgccat gctctccggc
     3721 cagtccggca aggtcatcct gagcctcgac tgatagccct tctaaccatg ggcgcgaccc
     3781 cgccgtatgg cggggtcggg ctatccgctc caagtcctcg cccgcgtccc cgcttcgcgc
     3841 ggccgcgggt tccgggcttt ccgctcctat ccctcgcgcg tcgcgcacgg acgcgcggcc
     3901 acggcccggc ccgtccgcct tggcggacgg gaaggtcacg gatgacgcct agcccaggaa
     3961 cgctatgacc gccacggccg cgacgatcgc gaccgccgtc gccgcgagga acgtaatcag
     4021 caccttatcg ctcgccttca tgaccctatc ctttcgtcgt cgccgcgcgc ctggcgcgcg
     4081 gcccaatacc cggccaggtc gtcgaggccg agacccagct cgtccatgcc cttccagagg
     4141 cgcggcagct cctcctcgag gaagcgccgc cgccgcccgc cgagcgcgac gtccctgccc
     4201 ccgtcggcga cgaagtagcc ggttccccgc tcgcagcggg cgacgccgtc gtcggcgagg
     4261 gcctgcaggg ccctcgcggc ggtattcgga ttgacgccca gcgaggccgc cagctcccgc
     4321 gccgagggta gccgggctcc cggacccagc cgtccggaga ggacgtcctc cgccaggagc
     4381 tcggcgatct gcgaaaagat cgggcgctcg ctactgaacc tcatcgcggg cttccttctc
     4441 ggcgaccctg aggtagccga acgcgatgga gaacgccggc acgagggcca ggcggacgac
     4501 gtcgacgagg acgcgcagcg tgcgctcgac cgagagggcg cccggcccgt caccgccgag
     4561 gacggtcagc gacccggaat cgtatgatac ggagaagccg cgggaacccg tagcctcgtg
     4621 gaccagcgat agacccaggt acacgaccag ggaacagagg agcacgaaag ctaccgacag
     4681 gccggcggtc ttcaggaagg cgcgcttgcg gaacgccgcg gagccggcca ggaaggccac
     4741 ggctacgacc gcgtactctc cgtaactacg gagcgcggcg agcgagatcg gatgccagat
     4801 cccggttccc ggccctccgg ccgcccgctc gaccagggcc agcacaaacg acagccccat
     4861 agcggccgcc gccgcgacga gcgggaagac gaccaggtac gatacgaatg cggccgcgta
     4921 cttctcgagc ggcgtcgccg gcagcaggat ccagtcggcg ccggtccgcc catgcatgcc
     4981 cttgaaggcc tgcgacgaca ggatgagccc ggagatgatg atcagccccg cgtagctcgg
     5041 atcgccctcg ctgttcatga aggcgcggtc ggcgataaga atagaaagaa gattcagccc
     5101 ggccacgatg gccagcccga tacccaggcc cggcgcgtcg tcataggccc tgttccgcgc
     5161 caggagcatg acccggcccg cgctgaagcg gctcgatagg gtatcgcggt tcatgccctg
     5221 cctcccgcgg ccagcgccgc tgcctcgggc cgatactcgg gtagcgcgtc cccgcgcagc
     5281 gcggccgcga gcctgtcggg cgccgcgatg gccgcgttga acacgagctc caggtccggc
     5341 ggcgccgcct cccccgggcc cgcggtcgcc aggagagcgg tccagccgac cgcgtcgcgc
     5401 tcggcgtaga cgacgggccg gccctcgatc gacggcagtc tggcggcgcc gagccgatcg
     5461 gacagctcgg ccatcgtcac cgtgaaggcc acccggcccc gatctatcac gatgatgggg
     5521 tcgagcagga gctcgaggtc gcggatctga tgggtcgaca cgacgacgac gcggtcgggc
     5581 ccagaagccg ccgccagcac gcgcctcagc tgcgccttgg acgggatatc gagcccgttg
     5641 gtcggctcgt ccaggaggag aagcctggag ccactcgcca ccgccgcggc caaggcgaat
     5701 ttcttacgct ggccatacga gaatttggat atcggcttat cggcgtccag tccgagctcc
     5761 gcgaacagct catcgaagcg cccgtgatcg aaggccggcc tgaacgcggc gtagcgccca
     5821 agccatgcgg ccggcttcag ggccgggaag cacggatcct ccggcacgaa acagacgtcg
     5881 gccagcgcgg ccgcctcccg ctttccggga gcccgtccgt agacctcgac gctgccccgc
     5941 tccggcctga gggcgccggc cgcgatcttc agaagactcg tcttaccggc cccgttgagt
     6001 cccaggagac cgtggatggc tcccggttcc aatcgtaggt cgaggtcctc gaagagcggc
     6061 ctcttcccat acccgaaggt catggattcc atacgtacca ttacctactc cttgagtgta
     6121 ctaggttatt aacacagtaa tacaaacacc gccacccgtc aagtccttgg aacgccgctc
     6181 gtagcgccat ggacgcgcgc cgatattgca tgcgggctca ggacgtgcga tagttcggcg
     6241 tatgaacgaa gaacgaaacg gcgccggccg gcgccgtgac ccgtcgcgcg atcgacggcc
     6301 agaagaacgc aaacagccgg atcaggctcg ccggacaaaa tacgtagcgc cgccgcgaac
     6361 ggtttacgaa ccgtcaagaa tgctcggcct cgagtccttc cggacgccga gcgccccgaa
     6421 agaggcggcg cacctcgtct ccaccctcct cgacatcgtc gaagaggtcg ccccgctccc
     6481 cgccggacgg cgcaaggaca tccgtcgcga cgtgctcgag ctatggcgcg agctgacgag
     6541 cgaaaaatcg acgcggcagc ccgactacat cggcgagccg gccaagctcg cggcctacct
     6601 gcgctatttt ctgccctgga acgtcgccag gctcgtcccg ctactcgtcg atctcgacct
     6661 cggccttaag ccgggcgacg ccgtactcga catcggttct ggaccgctga ccgtcccgat
     6721 agcgctctgg atagccaggc cggacctgcg cgccatggag cttcgcgtcg agtgcatgga
     6781 tagggtcagg cgcgcgatgg agatagggct ggggctcctc gaggggttag cgctacgctc
     6841 ggggcaagcc ctttcctgga agctaacgat acagaaagaa agcttctcga tcaccgacgc
     6901 ggccccgagg cgcgacttcg ccctcgtgac ctccgccaat gtcttcaacg agagcttctg
     6961 gcgcatcaaa ggatcgctct ccgaacgcgc cgacatgctc gcctcgtcgc tcggcgcccg
     7021 agcggctcgg ggcggacgta tcctgatcgt cgaaccgggc gatccgcgct ctggcgcgat
     7081 gctctcggcc ctccgcgaat cggtcatcct gcgcggaggc gagccgctgg cgccctgccc
     7141 acaccgggcg gcctgcccga tggccggcgc cttcctgagc tcggccttca gggactcgga
     7201 cgaagacggc cgcgacggtg gcgagcggct ggccaaggtg gtcaccgcca agggacgatc
     7261 caaggctccg tggtgccact tcgtcatcga tgccggcgcc gcgcctgagc ggctcgtggc
     7321 gttctccgag accgccggcc tacccaagga cagattgata gcctcgtggc tcctgctccg
     7381 gccggacgcc gccgagaaga ctccgagcgc gcaggcgccg tcgcgttcgg tgcgcctcgt
     7441 ctcggacgcg ttcaggctgg cggaaggcgg attaggccgt tatgcctgca cgaggactgg
//
LOCUS       DAOPHV010000053         7497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_12582_length_7497_cov_4.286213, whole genome shotgun sequence.
ACCESSION   DAOPHV010000053 DAOPHV010000000
VERSION     DAOPHV010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7497
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12582_length_7497_cov_4.286213"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..2270
                     /locus_tag="PLQ29_02445"
     CDS             9..2270
                     /locus_tag="PLQ29_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPG85533.1"
                     /translation="MAFVRRVPAAPASWAELPERLDDGLRAALDSRGVERLYSHQRAA
                     WDAVGAGMDIVVVTPTASGKTLCYNLPVAQALIDDPEARALYLFPTKALSQDQQAELN
                     ALVLGGDLPIKAATYDGDTPQSTRKAARDSGRIVISNPDMLHSGILPNHAKWIRFFAG
                     LRYIVVDEMHAYRGVFGSHVANVFRRLLRVAAYYGASPRFILCSATIGNPAELATALI
                     GRPVVAITDNGAPRAPKAVVFYNPPLVDPVQGIRRSSADESMSLAAKLLASGTRTILF
                     ARSRLRVEILASYLNKRFENVYNDNNRIRVEPYRSGLLPNERRQIERGLRDGSIHGVV
                     STNALELGIDIGGLDAAVIAGWPGNLASFWQQAGRAGRRSGASMAVYVASSSPVDQYM
                     IEHPERCFGSAAENARIDPDNPYIYADHVKCAAFELPFAKEERFGSDPGAVLSFLEEE
                     GTVRLAAGRYYWSDSGYPAEKISLRSATADNVVIIDTTAGRNEVLGEMDRPSAKELVF
                     DDAVYIHRGRQFIVLKLDIENRQCLVEERETNYYTDAVVKTDIKVLSEDERLGSAALP
                     IVLGDLLVRSSAEKFKKLRFHTNENIGYGEIYLPPEEMQTRAIALLFPEGGAAGSALA
                     AMGDGERAAALSAFCGILRALSPVELLCDKGDIGATWRLRDDHYAVPAVYVWDRYPGG
                     TGLAEALSSSLVPVLEAASGRIAACDCDDGCPSCVGLADDALQATGRRKSGGLALVRL
                     ALEALDGSSRSGR"
     gene            2275..3417
                     /locus_tag="PLQ29_02450"
     CDS             2275..3417
                     /locus_tag="PLQ29_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HPG85534.1"
                     /translation="MAGNLRARLARIREAAAEPQRPVPIHQASDDIATFPEGWETIGH
                     GLRFHESVRRNDAMAPPHAAIDLAIFSARLSGTIEAGNLLFFDLETTGLSGGAGTVAF
                     LAAFGSLRADGSLSIRQYFMDDYPFEPALIAAMGDEFARAGAVVSYNGSSFDLPLYAV
                     RRTMVGLGPAPDLLHADALHAARRLWRRSMPDCSLACLEAAALGVRRVGDIPGSEVPT
                     VWFDFLRVGPSDRLARVFAHNEQDIASLAALAFAVHDAAHGRRELPSVDAVGLAELRS
                     RVDEALAERTLTAALEAGDARATRPLMRLYRRTGRCGEIAPLVPFLPDDPAGLFSKSV
                     YAERRLRDRREALRLAELAEELGRGALRDRAARRAARLRAGQTAFR"
     gene            complement(3425..4519)
                     /gene="rlmN"
                     /locus_tag="PLQ29_02455"
     CDS             complement(3425..4519)
                     /gene="rlmN"
                     /locus_tag="PLQ29_02455"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPG85535.1"
                     /translation="MSAEPGNARSRRVGVAGLLPAEIASALPLSPAFRGKQVFKWIAK
                     GAAGFGDMTDLPAADRERLSSTPLYSSRVAERLEADDGSVKLKIALEDGAAVEAVLLT
                     DADGRRTACLSTQVGCRMACAFCKTGTLGLLRNLGPDEIVEQFVHLSALRGKPSNIVF
                     MGMGEPLDNLASLRKAIVILMHPEGFGVSKRKITISTCGIVPGIVDLADNGPHVRLAV
                     SLTAAEDALRSSLMPVNRSWNLAALKEALLYYQAKTGDRVTLEAAIMGGENSGVDSAA
                     AMAAWIRPLNAQVNVIPWNPVTGLPHREPARGEAEAFERELERRGVNAVRRARRGRGV
                     SGACGQLGDTLGSALSLESWLGSDDDDEAT"
     gene            complement(4516..5661)
                     /locus_tag="PLQ29_02460"
     CDS             complement(4516..5661)
                     /locus_tag="PLQ29_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG85536.1"
                     /translation="MSDQTNQHDEFEFDAGTAPQQNNALAEIAELSKKGYQYLKENRI
                     SDAERNFRKILEEDPENNYALVGLGDAARKRSSHRDAVDYYKKCLIYHPGNNYALFGL
                     ADCYKALNQYQKAIEIWEQYLLHDNKNITVLTRIADAYRKVRDFRKSKAIYLRVLEME
                     MDNPYALIGLGHLHYDFKEYRDALHYWQRMVDAQGDMVDIRVLTSIGNCYRKLKQFDQ
                     GVPYFEKALVKEPDNFYALFGLADCFRGTGEPQRSLDYWNRILDKDPRNKVILTRAGD
                     AYRSMDRFEEAADYYERALNIEFDVYAVLGLATISRLQGKVESAIESLKRLVQNDAKN
                     YRLHLELAQCYLAVGDRRKAEETLQDFMRLGIRNPHIQDLMAKIQNS"
     gene            complement(5658..5996)
                     /locus_tag="PLQ29_02465"
     CDS             complement(5658..5996)
                     /locus_tag="PLQ29_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85537.1"
                     /translation="MDERLREVFPDGGLIKTVDEPNGELSAEQKAHLNRRGNMLYNGG
                     DLETARRIFQTTGYSDGLIRVGESYMDAGRPVDALKMFKLAHDQARSAELIERAAMAI
                     RKMLDEKDTE"
     gene            complement(6346..6930)
                     /gene="rsmD"
                     /locus_tag="PLQ29_02470"
     CDS             complement(6346..6930)
                     /gene="rsmD"
                     /locus_tag="PLQ29_02470"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPG85538.1"
                     /translation="MSQGASTLRVTGGVLKGRRIQVPQGDYEIRPAMDRMRESIFSIV
                     GPLDGLSFLDLFSGSGIIALEAASRGASPVTCVERDRGKLGSLIKNVSIAPDRVECRC
                     MPVERFILRCERRYSLVFCDPPFPYAHKADIVRSIAERGVVEPGGLVMIHYPAEESLP
                     ESHAGLSLEDERAYGRSTVRFYRMAMAIDPRTAG"
     gene            complement(6927..7388)
                     /gene="smpB"
                     /locus_tag="PLQ29_02475"
     CDS             complement(6927..7388)
                     /gene="smpB"
                     /locus_tag="PLQ29_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPG85539.1"
                     /translation="MMEGVKIIALNRRARFDYVVEETLECGISLQGSEVKSLRIGKVS
                     FPDGYASFEDGELWLRNVHIAEYSCSSIFNHDPDRPRKLLAHAAELKRLERRVNEKGY
                     TLVPLDFHFRKGRVKVELGLCKGKKFADKRESMKERDVKRELQREFRERQR"
BASE COUNT         1264 a         2438 c         2383 g         1412 t
ORIGIN      
        1 cccccaatat tgccttcgtg aggcgggtgc cggccgcccc ggcctcctgg gccgaactgc
       61 cggagcggct cgacgacggg ctacgcgcgg ccctcgactc ccgcggtgtc gaacggctct
      121 acagccacca gcgggccgcc tgggacgccg taggggccgg tatggacatc gtcgtcgtca
      181 cgccgacggc gagcggcaag acgctctgct acaacctgcc ggtggcccag gccctgatcg
      241 acgacccgga agccagagcg ctgtacctct ttccgaccaa ggccctgtcc caggaccagc
      301 aggccgagct gaacgccctc gtgctcggtg gcgacctccc gatcaaggcg gccacctacg
      361 atggcgatac tccccagtcg accaggaagg ccgccaggga ctcgggtcgc atcgtcatca
      421 gcaatcccga tatgctccac tcggggatcc taccgaacca cgccaagtgg atcagattct
      481 tcgcggggct gcgttatatc gtggtcgacg agatgcacgc gtaccggggg gttttcggta
      541 gccacgtcgc caacgtgttc cgcaggctcc tccgcgtggc cgcctattat ggggcctcgc
      601 cgcgcttcat actatgctcc gcgaccatcg gcaatccggc cgagctggcg accgcgctga
      661 tcggccggcc cgtcgtcgcc atcaccgata acggggcgcc tcgggctccc aaggccgtcg
      721 tgttctacaa cccgccgctc gtggacccgg ttcagggcat caggcgcagc tcggccgacg
      781 agtcgatgtc gctggccgcc aagctgctcg cctccggcac gaggaccatc ctattcgccc
      841 gttccaggct gcgcgtcgag atactcgcct cctacctgaa taagcgcttc gagaacgtct
      901 acaacgacaa taatcgaatc cgggtggagc cataccgatc aggcctcctg ccgaacgaac
      961 gcaggcagat tgagcgcggc ctgcgggacg gctccatcca cggcgtcgtc tcgacgaacg
     1021 cgctcgagct gggaatagat ataggcggcc tcgacgcggc ggtgatagcc ggatggccgg
     1081 ggaacctcgc ctcgttctgg caacaggcgg gcagggcagg ccgccgctcc ggcgcctcga
     1141 tggcggtcta cgtcgcctcg agctctcccg tggaccagta catgatagag cacccggaac
     1201 gatgctttgg ctccgcggcc gagaacgcca ggatagaccc cgacaatccg tatatctacg
     1261 ccgaccacgt caagtgcgcc gccttcgagc tcccgttcgc caaggaggag cgcttcggct
     1321 ccgatcccgg ggccgtcttg tcgttcctcg aggaggaggg caccgtcagg ctggccgctg
     1381 ggcgctatta ctggtccgac tcaggctacc cggccgagaa aatatcgctg cgatccgcca
     1441 ccgccgataa cgtggtgatc atcgatacga cagctggcag gaacgaggtg ctcggagaga
     1501 tggaccgccc gagcgcgaag gaactggtgt tcgacgacgc cgtctacata caccgcggta
     1561 gacagttcat cgtgctcaag ctcgacatcg agaaccgcca atgcctcgtc gaggagcgag
     1621 agaccaacta ctataccgac gccgtcgtca agaccgacat caaggttctg tcggaggacg
     1681 agcggctggg ttcggccgcg ctgcccatcg tgctcggcga cctgctggta cgttccagcg
     1741 ccgagaagtt caagaagttg cgcttccata ccaacgagaa tatcggttac ggcgagatct
     1801 atctgcctcc cgaggagatg cagacacggg cgatcgcgct gctcttcccg gaaggcggcg
     1861 ccgccggctc cgccctcgcc gctatgggcg acggcgaacg ggccgcggcg ctatcggcct
     1921 tctgcggcat actacgcgcc ctgtcacccg tcgaactcct atgcgacaag ggagacatag
     1981 gggccacctg gcggttgagg gacgatcatt acgcggtgcc ggccgtgtat gtctgggatc
     2041 gctatcccgg cggcactggc ctggccgagg ccctatcgtc ttccctagtg ccggtgctcg
     2101 aggcggcctc cgggcgtata gccgcttgcg actgcgacga cggctgcccg tcctgtgtcg
     2161 ggctcgcgga tgacgctcta caggcgaccg gacgcaggaa gtcaggcggt ctggccttgg
     2221 tacgactggc cctcgaggcg ctggacggct cgtcccggag cggccgctga gcgtatggcc
     2281 ggtaacctgc gggcccgcct ggccaggatc cgcgaggcgg cggccgagcc gcagcgcccc
     2341 gtccccatcc atcaggcgtc ggacgatatc gccaccttcc ccgaaggctg ggagacgata
     2401 ggccacggcc tcaggttcca cgagagcgta cgacgaaacg acgccatggc cccgccccac
     2461 gccgctatcg acctcgcgat cttctcggcg cggctatcgg ggacgataga agccggaaac
     2521 ctactgttct tcgacctgga gacgacgggc ctatccggcg gagccggcac cgtcgccttc
     2581 ctagccgcct ttggcagctt gcgcgccgat ggctcgctat cgatccgcca gtacttcatg
     2641 gacgactatc cgtttgagcc ggccctaata gccgcgatgg gcgatgagtt cgcccgcgcg
     2701 ggcgctgtcg tcagctacaa cggctcgtcg ttcgacttgc cgctctacgc ggtcaggcgc
     2761 accatggtcg gcctcgggcc ggctcccgac ctcctccacg ccgacgcact gcacgcggcc
     2821 cgtcgtctct ggcggcgatc gatgccggat tgctcgctcg cctgcttgga ggccgcggct
     2881 ctcggcgtgc gtcgcgtcgg cgacattccg ggaagcgagg tgccgacggt gtggttcgac
     2941 ttccttcggg tcggcccgag cgatcgcttg gcgcgggtct tcgcccataa cgagcaggac
     3001 atcgcgtcgc tcgccgctct ggccttcgcc gtgcacgacg ccgcccacgg taggcgggag
     3061 ctaccatccg tcgacgccgt cggtctcgcc gagctacgct ccagggtcga tgaggccctg
     3121 gccgaacgca ccctgaccgc agcgctggag gcgggtgacg ccagggcgac gcgcccgctc
     3181 atgaggctgt accgtcggac cgggcgttgt ggcgagatcg cgccgctcgt gccattccta
     3241 cccgacgacc cggccgggct gttctcgaaa tcggtctacg ctgagcggcg cctccgcgac
     3301 cggcgcgagg cgctcaggct ggcggaactg gccgaggaac tcggtcgagg agccctgcgc
     3361 gatagggccg cgaggcgggc agcccgcctg cgcgcggggc agacggcgtt tcgttgatcg
     3421 ttctctaggt agcttcgtcg tcgtcgtctg agccgagcca ggactcgagg ctcaaggcgg
     3481 atccgagcgt atccccgagc tggccgcagg ctccggaaac acccctgccg cgccgagccc
     3541 tgcgtaccgc gttgacgccc ctacgctcca gctccctttc gaaggcctcg gcttcgcccc
     3601 tggccggctc gcgatggggt aggccggtca ccgggttcca cggtatcaca ttgacctggg
     3661 cgttgagcgg gcgtatccac gcggccatgg ccgcggcgct gtcgactcct gagttctcgc
     3721 cgcccatgat ggccgcctcg agcgtcacgc gatcgccggt cttggcctgg taatagagca
     3781 gggcttcctt gagcgcggcg aggttccacg agcgattgac cggcatcagc gagctacgca
     3841 gagcgtcttc tgccgcggtg agcgataccg ccagccgtac gtgcgggccg ttgtcagcga
     3901 ggtcgacgat gcccggcacg atgccgcagg tcgagatggt tatcttgcgc ttggatacgc
     3961 cgaagccttc cgggtgcatc aggatgacga tggccttcct gagcgacgcg aggttgtcga
     4021 gcggctcgcc catgcccatg aacacgatgt tcgacggctt gcctcgcagg gccgacaggt
     4081 gtacgaactg ctcgacaatc tcgtcgggac cgagattgcg caataggccg agagtaccag
     4141 tcttgcagaa cgcgcaggcc atgcggcatc cgacctgggt ggaaaggcac gccgtgcggc
     4201 ggccgtcggc gtcggtcagc aggacagcct cgaccgcggc tccgtcttcc agcgcgatct
     4261 tgagcttaac cgatccgtcg tccgcctcca gcctttcggc gacgcgcgac gagtagagcg
     4321 gcgtcgacga aagacgctcc ctgtcggcgg ctggaaggtc ggtcatgtcg ccgaaaccgg
     4381 ccgcgccctt ggctatccac ttgaatacct gcttcccgcg gaaggccggc gacagcggca
     4441 gggccgaggc tatctcggcg ggaagcaagc cggcgacgcc gacccgccgg gaccgggcat
     4501 ttccgggctc ggcgctcacg agttctggat cttggccatc agatcctgga tatgcgggtt
     4561 gcggataccg aggcgcatga agtcctgtag cgtctcctcg gccttgcgcc tatcgccgac
     4621 ggcgaggtag cactgggcca gctcgaggtg tagacgatag ttcttggcgt cgttctggac
     4681 caggcgcttg agcgactcga tagccgactc gaccttgccc tgaagcctcg agatggtagc
     4741 caggccgagc accgcgtaca cgtcgaattc gatgttcaag gcgcgctcgt agtaatcggc
     4801 cgcctcctcg aagcggtcca tgctcctata ggcgtctccg gccctcgtca ggatgacctt
     4861 gttgcgtgga tccttgtcca atatccggtt ccagtagtcg agcgaacgct ggggctcgcc
     4921 ggtgccccgg aagcaatcgg ccaggccgaa caacgcgtag aaattgtcag gctccttgac
     4981 cagagccttt tcgaagtacg ggacgccctg atcgaactgc ttgagcttgc ggtagcagtt
     5041 gcctatggag gtcagcaccc tgatgtcgac catgtcgccc tgggcgtcga ccatgcgctg
     5101 ccagtagtgg agcgcgtccc ggtattcctt gaaatcgtag tgaaggtggc cgagaccgat
     5161 aagggcgtag ggattgtcca tctccatctc gagcacgcgc aggtagatgg cctttgactt
     5221 cctgaaatcg cggaccttgc ggtaggcgtc ggctatccgc gtcagtaccg ttatgttctt
     5281 attgtcgtga aggaggtact gctcccagat ttcgatggcc ttttggtact ggttgagcgc
     5341 cttatagcag tcggccaggc cgaagagggc gtagttgttg cccgggtggt agatcagaca
     5401 tttcttgtaa tagtcgacgg catcgcggtg cgacgagcgc ttcctggccg cgtcgccgag
     5461 gccgacgaga gcgtagttgt tctccgggtc ctcctcgagg atcttcctga aattgcgctc
     5521 cgcgtcgctt atccggttct ccttgaggta ctggtatccc tttttggaca gttccgcgat
     5581 ttcggcgagc gcgttgttct gctggggggc cgtcccggcg tcgaactcga actcgtcatg
     5641 ttggttggtt tggtcgctca ttctgtatcc ttctcgtcta gcatttttct tatcgccata
     5701 gccgctctct cgatcagctc ggcgcttcgc gcttggtcat gggctagctt gaacatcttc
     5761 aacgcgtcga ctggccgacc ggcgtccatg taggactcgc cgacgcgaat aaggccgtcg
     5821 gagtaaccgg tcgtctggaa gatgcggcgg gccgtctcca ggtctccacc attgtagagc
     5881 atattcccgc gccggttaag atgagccttc tgttcggccg atagctcgcc gttaggctcg
     5941 tcgaccgtct ttatcagtcc gccgtcgggg aaaacctcac gtaaacgctc gtccatcggg
     6001 actccgtgac cggcgaagcg ccgagaggcg cgccggttgt tttggtattg aagaaaatat
     6061 agttgacttc atataaataa tcaattcact tgcgaggata cggtcttgat gaaaaataat
     6121 cggcgcgtga tatagttttt tttagtaacc ggatgaatca tcatgcgggg cggccgtaac
     6181 gccccccggg acgggagcgc ccgatgctat gtgacgaact cggtctctac gcggctgact
     6241 cgatgtacgc cgaggactcc gaacaggctc tgcctccctc cgagactcgt gtcgtggacg
     6301 agcccatcgg aacggaccgt tccatgctgg actaccggct gatagctatc ctgcggtcct
     6361 tggatcgata gccatcgcca tacggtagaa gcgaaccgtc gatcggccat aggctcgctc
     6421 gtcctcaagc gatagcccgg cgtgcgactc cggcaagctt tcctcggcgg ggtaatggat
     6481 catgacgagc ccgcccggct cgaccacgcc gcgctcggct atggagcgga cgatatcggc
     6541 cttatgggcg tacgggaagg gcggatcgca gaagacgagc gagtagcgcc tctcgcatcg
     6601 cagtatgaag cgttcgacag gcatgcacct gcattcgacg cgatccgggg cgatggaaac
     6661 gttcttgatg agcgagccga gcttgccgcg gtctcgctcg acgcaggtga ccggagacgc
     6721 tccgcggcta gccgcctcga gggctatgat gcccgagccc gagaacagat cgaggaacga
     6781 tagcccgtcg agcggcccga cgatggagaa gatcgactcg cgcatcctgt ccattgccgg
     6841 tcggatctcg tagtcgccct gcgggacctg tatccggcga cccttgagga cgccgccggt
     6901 aaccctgagc gtactggccc cctggctcat cgttgccttt ccctgaactc ccgctgcagc
     6961 tcgcgcttga cgtcgcgttc cttcatcgac tcgcgcttgt cggcgaattt cttgcccttg
     7021 cacaatccta gctcgacctt gacccgcccc ttcctgaagt ggaagtcaag ggggacgagc
     7081 gtgtagccct tctcgttgac cctgcgctcg aggcgcttca gctcggccgc gtgggctagg
     7141 agcttccgcg gccggtcggg atcgtggttg aagatcgaac tgcatgagta ctcggctatg
     7201 tgtacgttcc gtagccaaag ctcgccgtcc tcgaacgagg cgtagccgtc ggggaacgat
     7261 accttgccta tcctaagcga cttgacctcc gagccttgga gcgatatgcc gcattcgagc
     7321 gtttcctcga cgacatagtc gaaacgcgcg cgccggttca gcgctatgat cttgacgcct
     7381 tccatcatcg ctccgccgga accatcgcca cgcggaagcc gaggtacgcg ctacagccgc
     7441 tcgccctgac aggcccgcga gacgacaggg taacgagatc ggcccgattg gcccacg
//
LOCUS       DAOPHV010000054         7479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_12632_length_7479_cov_3.955819, whole genome shotgun sequence.
ACCESSION   DAOPHV010000054 DAOPHV010000000
VERSION     DAOPHV010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12632_length_7479_cov_3.955819"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PLQ29_02480"
     CDS             complement(<1..656)
                     /locus_tag="PLQ29_02480"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPG85540.1"
                     /translation="MKRFVASLALLALGAVAFAQAEPDWYVGKTIKDIRFDGLVVVPM
                     KDLEPIVKEFRGKPFDDQLWMNLLARVYETDYFDAVAPEAVPSDQAATAVIILFKVKE
                     KPAVTSVAIVGNSGIRTQEILDALSVKERTIFNPSRMRLDEISLRRLYQEKGFPEIKV
                     TAASSTLADGGVAVTFTVAEGVQNIVESIRFEGLESVSESSLKGEIKLKEKGLFQSGQ
                     "
     gene            complement(671..5041)
                     /locus_tag="PLQ29_02485"
     CDS             complement(671..5041)
                     /locus_tag="PLQ29_02485"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPG85541.1"
                     /translation="MKLRAPAPIVIAAIVVLAVSAAWAVVAPPLLGRMESAAAALIER
                     EAAYAIRGLGLSASYRAASRRLLGSIELRGVELRGGESVVARVRRLQLRYGLGALLGG
                     RFELTGVRVDGVSMAAELQDAKALVERLASALPGTGSSASEGAASLAVELRRLTLTLE
                     LGDGATATLSLRAADLLLDGPGGVRASAAGSIRASDPSGRYLATAVELPFSCTASVRS
                     PGGQGSGLEASIAAVIAGDSDLGRLSRARIDATLDDTGLSASVEPGAGIERLRFDWNP
                     STGLAKLDLAVTRWSPASLFYPAGRLSGARPWLESSYSGSLRVSSDLSAEGTSVAADL
                     SGVIPLALPGGRPRATLSASGGWDTLRVRSATLRNGSLGAEFSGELSLGRLSADGRLS
                     ASYALGPGLEASADFQLSGAGRSWFAYAPAIRAADATLGDAIVSLELSSSSGALDQAS
                     FYLDASLPTIADADGDPAVEPVDSPGIVEASSARAASRLTIEGSASLGEEPYLEAVAR
                     VGTLSLEAFPGLLTALVGAGAADALAPLSVGGELSLYSDLSGLSYNSSGLLVVYDGAI
                     KGFGMLGFSGGLDRLRISSIDATIGGYSVKGSASLEYGASSGPSFAADLRVEDVPYGL
                     SGAMADGAVFVSGDYGLRFVGNGRDDEYVASLDIDQMPIPLFGSVSFLSAAADGRFSS
                     TDDWNLVFESLALEQPYGSASRLPSVSLAGAFDESGGRVGSLGYRDAVSSLNGSATVT
                     WTLSGGFRVSGNARMAGPAGEYYALDGEYASDGTMDAALSTRKAPLARLYLPWLRGVV
                     DADARIGGSIRDPSARFSFTVNGGQRADNLPFAAGAGTFERGVLSLVDSRVRLGDQAV
                     SGLTLSYGVGDAAASLAANVELAMGRNRLTGRLVAVGASNGAGPSPFGDYLVAGELSG
                     ASWTGGELGDIPFSLGLADGDASLSMGPSGELEASLGAAGELRASLDRSMPISLEAQG
                     VLADGVINVDVRDAVVDLPFLFKLIGLPMLGADSGVGRGAVTIRGKAVDPSVEGSFEL
                     EGFYLSLPDYVSEPIGPLEAPLFFTGRTMETLQTDVPCGDAVLTVALEGVLNQGIPDD
                     VHLTVSTSGLGLVPVSTRLLGLDIDGLARPELVIDANRDRSRMTGSIMLASGDVVITP
                     GVVSGRMQASRGAGILEPVEFTGALDISFGKAVRVYFPDKRLPVIYGLTDPSSRLSVD
                     FDAALGEFNLDGQAVLRGGSVFYIQRNFYLKHATIDFDEDAEQFDPLINAEAETRTSS
                     ESGPVVVTLRAVDSRLSSLSFSLESSPSMAEDTILQMLGQNLIGAQGGSADLGRFVFE
                     NSDLIPQLNVMSVLERNLHGLLGLDLFVVRSQVFQRWLYDVSGLSGSSGASSLADYLD
                     ETAIVGGKYIGDKLFAQLALSLVADPLASSAALSLDSEFSLEWKAPHFTLNWSIEPEH
                     PDSLFIEDQSFSFLWRIPLQ"
     gene            5109..5756
                     /gene="tmk"
                     /locus_tag="PLQ29_02490"
     CDS             5109..5756
                     /gene="tmk"
                     /locus_tag="PLQ29_02490"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPG85542.1"
                     /translation="MQQTRTILEGFVALEGIDGAGTTTQLRRLSARLEAAGVAFSTEC
                     EPTPGPVGRLIRQALSGAFDARPETVARLFAADRGEHLYGPGGILERTAAGSLVVSDR
                     YLFSSLAYQGLTCGPDLPAELNAPFPLPELLLFFELDPEIAAERMAARDSLEIYENLA
                     FQRRVDAAYRAVVSSFEGSGMAVVRVDAAASPDEVERTLWSAIEPISMARARRYS"
     gene            5760..6359
                     /locus_tag="PLQ29_02495"
     CDS             5760..6359
                     /locus_tag="PLQ29_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85543.1"
                     /translation="MKRTVALALVLIFSSLSLSGYPVYFKEQYYRLYHTHYIQYPDDT
                     IENIYWLEQARKADFCNPLYALAKIADERQWEKYRYLFNMHIELKIIEQHLYLGAKFD
                     KRVAYFYNAPWKRENLDSLATAETAYKAALAYWPSAKAWAEKAAGIRFMYLTDVQAWE
                     DEAYRIAEGSLDYGRVIERELERLAKVRAAFEAMDEAGY"
     gene            6356..6979
                     /locus_tag="PLQ29_02500"
     CDS             6356..6979
                     /locus_tag="PLQ29_02500"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HPG85544.1"
                     /translation="MIADRPALLVTGGDAPPFSALSGRLGEFLYICAADSGLDTLRSW
                     GLRADLAVGDMDSLADRSLLDGCAEVLAFDRDKDDTDTELGLRELRARGYGPITMAGG
                     GGGRLDHLLALRALLERPSGPDEWLCAGERVVRLEEKRSFVLAVGATVSVFPLSGGAS
                     GMRSVGLKWPLDGLSWGSGGFGVSNVAVSSMVTIDPGQRPIFVVLPL"
     gene            complement(6980..7465)
                     /locus_tag="PLQ29_02505"
     CDS             complement(6980..7465)
                     /locus_tag="PLQ29_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPG85545.1"
                     /translation="MDYFFNFDKMEYLTEKRPDGCILCLVRDASPEVKRLVVAESEYS
                     MATLNLYPYNPGHLMVFPKRHLSDIRELGDAERADLDALVRRSLDALDSTHGPGGYNV
                     GCNMGLVAGASIEHLHVHIIPRYPREIGIAELMAGKRVLVQDPLETQRLLVEAFARLP
                     G"
BASE COUNT         1300 a         2509 c         2504 g         1166 t
ORIGIN      
        1 aactggccgc tctggaacag ccccttctcc ttgagcttga tctcgccctt gagcgacgac
       61 tcggagacgg actcgaggcc ctcgaacctg atggactcga cgatattctg gacaccctcg
      121 gcgacggtga aggtaaccgc gacgccgccg tctgccagcg tcgagctggc cgccgtcacc
      181 ttgatctcgg ggaagccctt ctcctgatac agccttcgca gcgatatctc gtcgagccgc
      241 atccgcgacg ggttgaagat ggtccgctcc ttgaccgaca gggcgtccag aatctcctgg
      301 gtacgtatgc cggagttgcc gacgatcgcc acgctcgtca cggcgggctt ttccttgacc
      361 ttgaacagga tgatgacggc cgtcgctgcc tggtcggacg gcacggcctc gggggccacc
      421 gcgtcgaaat agtcggtctc gtagaccctg gccaagaggt tcatccagag ctggtcgtcg
      481 aacggcttgc ccctgaattc cttgacgatg ggctcgagat ccttcatcgg gacgacgacg
      541 agcccgtcga acctgatatc cttgatcgtc ttgccgacgt accagtccgg ttccgcctgg
      601 gcgaacgcta cggcgccgag cgctagcagc gcgagagaag ctacaaatcg cttcatatcg
      661 atggccttcc tcattgtagg gggattctcc aaaggaacga aaatgattga tcctctatga
      721 acagcgagtc cgggtgctcc ggttcgatac tccagttcag cgtaaaatgc ggagccttcc
      781 actcgaggct gaactcggag tctagactca aggcggcgct cgatgcaagc ggatcggcga
      841 cgagggacag ggccagctgg gcgaacagct tgtcgcctat gtactttccg ccgacgatgg
      901 ccgtctcgtc gaggtagtcg gccaggctcg aggcgccaga cgagccggaa agccccgata
      961 cgtcgtagag ccagcgctgg aagacctgcg agcgcacgac gaacaggtcg aggccgagga
     1021 ggccgtgtag gttacgctcg agcaccgaca tgacgttcag ctggggtatg aggtcgctat
     1081 tctcgaagac gaagcggccg agatcggcgg agccgccctg ggcgccgatg aggttctgcc
     1141 cgagcatctg caatatcgtg tcctcggcca tgctcggcga cgactcgagc gagaaggata
     1201 ggctcgatag cctgctgtcg accgcgcgga gcgttacgac gacagggccc gactccgagc
     1261 tggtcctcgt ctcggcctcc gcgttgatca acgggtcgaa ctgctcggcg tcctcgtcga
     1321 agtctatcgt cgcgtgcttg aggtagaagt tacgctggat gtagaagacc gagccgccgc
     1381 gtagcacggc ctgcccatcg aggttgaact cgccgagggc tgcgtcgaaa tcgaccgaga
     1441 gccgactcga cgggtcggtc aggccataga tgacgggcag gcgcttatcg gggaagtaga
     1501 cgcggaccgc cttgccgaac gatatatcga gggctccggt aaactccacg ggctcgagga
     1561 tcccggcacc acggctcgcc tgcatccgtc cgctgacgac cccgggcgtg atcacgacgt
     1621 cgccggaagc cagcatgatc gagccggtca tgcgcgatcg gtcgcggttg gcgtcgatga
     1681 ccagctccgg cctcgcgagg ccgtcgatgt cgagcccgag cagcctggtg gacacgggca
     1741 cgagccctag ccccgaggta gagacggtca ggtgaacgtc gtcgggtatg ccctggttca
     1801 agacgccctc gagcgcgacg gtcaggaccg cgtccccgca tggcacgtca gtctgcagcg
     1861 tctccatggt ccggccggtg aagaacagcg gcgcctcgag cgggccgatc ggctcggata
     1921 cgtagtccgg gagcgacagg tagaagccct cgagctcgaa ggagccctcg accgacggat
     1981 cgacggcctt gccgcgtatg gtgacggccc cccggccgac gccggagtcg gcgccgagca
     2041 tcggcaagcc tatcagcttg aacaggaagg gaaggtcgac gacggcgtcg cgaacgtcga
     2101 cgttgatcac gccgtcggcg agcacgcctt gggcttcgag cgatatcggc atggaacgat
     2161 cgaggctcgc cctgagctcg ccggcggctc cgagcgaggc ctcgagctcg ccggacgggc
     2221 ccatcgacag gctcgcgtcg ccatcggcta gcccgaggga gaacggtatg tcgcccagct
     2281 cgccgccggt ccacgaagcc ccggacagct cgcccgcgac taggtagtcg ccgaacggtg
     2341 acggcccggc gccgttggag gcgcctacgg cgaccagcct accggtcagt cggttccggc
     2401 ccatggccag ttcgacgttc gccgccagcg aggccgcggc gtcgccgacg ccgtagctga
     2461 gcgttagtcc ggagaccgct tgatcgccca agcggacgcg agaatcgacc aagctgagca
     2521 ctcccctctc gaaggtcccg gcgccggccg cgaacggcag gttgtcggcg cgttgaccgc
     2581 cgttcacggt gaaactgaag cgggcggatg gatcccgtat cgagccgcct atcctggcgt
     2641 cggcatcgac gacgccgcgt agccagggta ggtacagcct ggcgagcggc gccttcctcg
     2701 tcgatagcgc cgcgtccatg gtcccgtcgc tagcgtactc gccgtcgagc gcgtaatact
     2761 cgccggcggg gccggccatc ctcgcgttgc ccgacactcg aaaaccgcct gacagggtcc
     2821 aggttacggt agccgatccg ttgagcgacg atacggcgtc ccgatagccg agcgaaccga
     2881 cgcggccgcc ggattcgtcg aaagcgcccg ctagggagac cgacggcagg cgcgaggcgg
     2941 agccgtaggg ttgctcgagc gccagcgact cgaagacgag gttccagtcg tcggtcgagg
     3001 agaagcggcc gtcggcggcg gccgacagga aggatactga cccgaacaac ggtatgggca
     3061 tctggtcgat atcgaggctg gcgacgtact cgtcgtcgcg tccgttaccg acgaagcgca
     3121 agccgtagtc cccggagacg aagacggccc cgtctgccat ggcgccagat agcccgtagg
     3181 gtacgtcctc cacgcgtaga tcggcggcga acgacgggcc ggacgaggca ccgtactcga
     3241 gcgaggccga gcccttgacc gagtagccgc cgatcgtcgc gtcgatcgac gatatccgca
     3301 gtcggtccag ccctccggag aagccgagca tgccgaagcc cttgatcgcg ccgtcgtaaa
     3361 cgacgagcag gcccgacgag ttgtaggaca gcccggagag gtcgctgtac agcgacagct
     3421 cgcccccgac gctgagcggc gccaacgcgt ccgcggcgcc ggcgccgacc aaggcggtca
     3481 ggagcccggg aaaagcctcg agcgacaggg tgccgacgcg ggcgaccgcc tcgaggtagg
     3541 gctcttctcc gagcgaggcg gagccctcga tggtcagcct cgaggcggct ctcgccgacg
     3601 aggcctctac gatgcccggg ctgtcgaccg gctcgacagc cggatcacca tcagcgtcgg
     3661 cgatagtcgg tagcgacgcg tcgaggtaga acgaggcctg gtcaagcgct ccggatgacg
     3721 aggacagctc gagcgatacg atcgcgtcgc ctaaggtcgc gtccgcggcg cgtatggccg
     3781 gggcgtaggc gaaccacgac cgcccggccc cggacagctg gaaatcggcc gacgcctcga
     3841 ggcccggacc gagcgcgtag gaagccgata gccgcccgtc ggcgcttagc cgcccgagcg
     3901 acagctctcc ggagaattcg gcgccgagcg agccgttacg cagggtggcc gaacgcacgc
     3961 gcagggtgtc ccagccgccc gaggccgaca acgtggcccg cggcctaccg cctggcaacg
     4021 cgagcgggat gacgccgctc aggtctgccg cgacgctcgt gccctcggcg ctcaggtcgc
     4081 tcgagacgcg gagcgagccc gagtacgacg actcgagcca gggccgtgcg ccggacaggc
     4141 gacccgcggg atagaacagc gaagccgggg accagcgcgt gacggccagg tcgagcttag
     4201 ccagccccgt ggaagggttc cagtcgaagc gtagccgctc aatccccgcg ccgggttcga
     4261 cgctcgcgct caggccggta tcgtcgagcg tcgcgtcaat ccgcgcgcgc gatagccggc
     4321 ccaagtcgct atcgccggcg attaccgcgg cgatgctcgc ctccagcccg gagccctgcc
     4381 cgccggggct ccgtaccgag gcggtgcacg agaacggcag ctcgacggcg gtcgccaggt
     4441 atcgaccgct cggatcggag gcgcgtatcg acccggcggc gctcgcccgt acgccgccgg
     4501 ggccgtccag cagcaggtcg gcggcccgca gcgacagggt cgccgtcgca ccgtcgccca
     4561 gctcgagggt cagggtcagc ctgcgtagct cgaccgcgag gctagccgcc ccctcggacg
     4621 ccgaggatcc ggttccaggc agggcggacg ctagccgctc gacgagggcc ttcgcgtctt
     4681 ggagctcggc cgccatcgac acgccgtcga cgcgtacgcc cgtcagctcg aaccggccgc
     4741 cgagcaaggc gccgagtccg taacgtagct ggagcctccg aacccgcgct acgaccgatt
     4801 cgccgccgcg cagctctact ccacgtagct ctatcgagcc gaggaggcgc cgcgaggcgg
     4861 cccggtagga ggccgacagc ccgagtcccc gaatggcgta ggccgcctca cgctctatca
     4921 gggccgcggc cgccgactcc atgcgcccga gcagcggcgg cgcgacgacg gcccaggccg
     4981 ctgacacggc gagtacgacg atggcggcta tgacgatggg cgccggggct cgaagcttca
     5041 cgggatccac agtaccacag cgccgggcct tgcgccatat cgcgcctcgg ggctatgctc
     5101 cctctccgat gcaacagact agaaccatac tggaaggctt cgtcgcgctc gagggcatag
     5161 acggcgccgg cacgactacc cagctccggc gcctctcggc caggctcgaa gccgcgggcg
     5221 tcgctttctc gaccgagtgc gagccgactc ccggccccgt cgggcgcctg atcagacagg
     5281 cgctatcggg cgccttcgac gcgcggcccg agacggtggc ccgcctgttc gcggccgatc
     5341 gcggcgagca cctctacggt cccggcggca tactcgagcg aaccgccgcc gggagcctcg
     5401 tcgtcagcga ccgctacctc ttctcgtcgc tggcctacca gggtctgacc tgcgggccgg
     5461 acctgccggc cgagctcaac gctcccttcc cgctacccga actactccta ttcttcgagc
     5521 tcgacccgga gatagccgcc gagcgcatgg ccgcacgaga ctccctggag atctacgaga
     5581 acctcgcgtt ccagcgccgg gtcgacgccg cctaccgcgc ggtcgtctcg tccttcgagg
     5641 gctccggcat ggccgtcgtc agggtcgacg ccgccgcgtc gcccgacgag gtcgagagaa
     5701 cgctctggtc ggcgatcgag cctatatcga tggcgagagc gcgccgatac tcgtaatgga
     5761 tgaaacgaac ggtcgccctc gcactagtgc tcatcttctc gtcgctgtcc ctgtcggggt
     5821 atcccgtata cttcaaggaa cagtactacc ggctctacca tacccactac atccagtatc
     5881 ccgatgatac gatcgagaac atctactggc tcgagcaggc caggaaggcg gacttctgca
     5941 atcccctgta cgccctggcc aagatcgccg acgaacgcca atgggagaag taccgctatc
     6001 tgttcaacat gcacatcgag ctcaagatta tagagcagca cctgtacctc ggggctaagt
     6061 tcgacaagcg agtcgcgtac ttctacaacg ccccctggaa gcgggagaac ctcgatagcc
     6121 tggctaccgc ggagaccgcc tataaggcgg ccctcgccta ttggccgtcg gctaaagcct
     6181 gggccgagaa ggccgcaggc atacgcttca tgtacctgac cgacgtacag gcctgggagg
     6241 acgaagcgta ccgcatcgcc gagggtagcc tggactacgg ccgcgtcatc gagcgcgagc
     6301 tcgagcgcct cgcgaaggtc agggccgcct tcgaggcgat ggacgaggca ggatattgat
     6361 agccgatagg cccgccctgc tcgtcaccgg cggcgacgcg cctccgttct ccgccctatc
     6421 cggccgcctc ggggaattcc tctatatttg cgccgccgac tcgggcctcg atacgctgcg
     6481 ttcctggggt ctgcgggccg acctggccgt tggcgacatg gactcgctgg ccgatcgatc
     6541 gctactcgac ggctgcgccg aggtgctcgc ctttgaccgc gacaaggacg atacggatac
     6601 cgaactcggc ctcagggaac tgcgcgcccg cggctacggc ccgatcacca tggccggcgg
     6661 cggcggcggc aggctcgacc acctcctggc gctccgggcc ttgctcgagc ggccgagcgg
     6721 ccccgacgag tggttatgcg ccggggagcg cgtggtcagg ctcgaggaga agcgtagctt
     6781 cgttcttgcc gtcggagcga cggtctcggt gttcccgtta tcaggcggcg cctcgggcat
     6841 gcgtagcgtc ggcctcaagt ggccgctcga tggcttgtcg tggggttcgg gcgggttcgg
     6901 ggtatcgaac gtggccgtct cgtccatggt gaccatagac cccggccaac gcccgatctt
     6961 cgtcgtgctg cccctctagt cagcccggaa gcctcgcgaa cgcctcgacg agtagccgct
     7021 gcgtctcgag cgggtcctgg accagcacgc gcttaccggc catcagctcg gctatgccta
     7081 tctcgcgcgg gtaacgaggt atgatatgga catgcaggtg ctcgatgctg gctccggcga
     7141 cgaggcccat gttgcagccg acgttgtagc cgcccggccc atgcgtcgag tcgagcgcgt
     7201 cgaggctgcg cctgacgagg gcgtcgaggt cggcccgctc ggcgtcgcct agctcgcgta
     7261 tgtccgatag atgccgcttc gggaagacca tcaggtgccc gggattgtag gggtacaggt
     7321 tgagcgtcgc catggagtat tcagactccg cgacgaccag gcgcttgacc tcgggagaag
     7381 cgtctcgcac gaggcagagg atgcagccgt cggggcgttt ctcggtcagg tactccatct
     7441 tgtcgaagtt gaagaaatag tccatgcgtc acttcccgg
//
LOCUS       DAOPHV010000055         7369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_12868_length_7369_cov_3.948728, whole genome shotgun sequence.
ACCESSION   DAOPHV010000055 DAOPHV010000000
VERSION     DAOPHV010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12868_length_7369_cov_3.948728"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1783
                     /locus_tag="PLQ29_02510"
     CDS             <1..1783
                     /locus_tag="PLQ29_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPG85546.1"
                     /translation="VSDVRRESAASDKGAMRLTLIFVIDEGRRYTYGGMGFAGNSLYT
                     SEQLGALVRQQPGAVLNYERLLQDQSRAADLYFENGYIFNGFRLAETRDEELGSIAYT
                     LVIDEKPQAHIEDVIFRGNVKTKEFVLRRLVPLESGDVFSKAKVLESLRNLYNTQYFS
                     SIVPEYEQGSQDLFVDLIISVEEQSTASIQFGMTYSPGTSAGEFPIIGLVNWSDINFL
                     GNGQTVALKTNLAVDSQDLTFSFSDDWLFGKRLSGSLEFAFEHEYLEAAQDSTGVIFP
                     YGDPTRVPDPYNSYEEYVAAGKLVPDGYKMTYDTWTFSLGYSGGYRWNTKLGTAGVVP
                     GVTHELGMKTYDSDLYRPYDSTISENLDTWLFSNTVYARAYLNKLDLWYDPSRGYYAS
                     QRLGLTGWFDGEINHFLRSDTRADAFVTLFDLPITESYSFKGVLGAHSKFSALIAQPW
                     YDDGLVASDSKKLNIDGTFVGRGWSSLGSYYGTALWDNWLEFRMPIVPSVLSLDGFLS
                     AAMLSTESGLLDVAGVDSGSTAYGGTLSELGLNNFAFSAGVGLRFIILQFPFRIYFAK
                     KFIYDDGFAWSSDDDWQFVLSVTTSLD"
     gene            1806..2336
                     /locus_tag="PLQ29_02515"
     CDS             1806..2336
                     /locus_tag="PLQ29_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPG85547.1"
                     /translation="MKKTAIIGLALFALCAQAFGQQITRVGVIDLQKVYTTYYKDSQA
                     VRALEEEKARVNEEIKRLSEEIKELQKRRLGLLSSADAAALKTFDEELYRKAQFLSDF
                     VKIKQADLDAKAESMTKGDAFVQMLYRTVQAISEAEGFSLVISSRNASDVGSSVIWFS
                     PMIDITDKVIQALLGA"
     gene            complement(2347..2838)
                     /locus_tag="PLQ29_02520"
     CDS             complement(2347..2838)
                     /locus_tag="PLQ29_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPG85548.1"
                     /translation="MEEVLYIKETNNRVYIRAQGHITANACPELKARVFDRLDAKPAV
                     EDVIIDLSSCEYMDSTFMGLIVGFNKRFMRFSGRPIRLAGLSETCMKLLKTIGIVRLV
                     TIEKEPPAFPEPLERLGAEAKAGAGLVLRAHEELIELSEENERRFSALRDALKGVAGA
                     DQD"
     gene            2932..5532
                     /gene="mutS"
                     /locus_tag="PLQ29_02525"
     CDS             2932..5532
                     /gene="mutS"
                     /locus_tag="PLQ29_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPG85549.1"
                     /translation="MAEQVPLLEQYDRIKALHRDEILFFRLGDFYEMFYGDAVEASSL
                     LGLTLTHRQEAPMCGIPHHAARGYIARLLRAGKKIAVCEQLSAPGRGKGVIERGVVEV
                     VTPGSVVEDDYLESGANNYIVAIGAFGDDMAIAWADASTGEFKAGSFGRGDPERLRRE
                     LFRLSPREALVRESTLDDDGVARALSERPAIVLNRFPDWTFSLEQGRSTLLGHFGTSS
                     MKGFGFDDGDPALAAAGALLEYLRDSVKARLSQFRTLGGCDDAEFVSIDESSQKNLEL
                     DRNLRDGGAAFTLYGVVNHTKTAMGARALRSRLQRPLRSAAAVNGRLDAVDALYRDQR
                     SLERVRAILGSCRDLERLASRLAMDRASPKDLVALRDTIRAALSLGPALPIAAPTALS
                     TLAEPGDRDAALAFADVIARAVVDDPAMSPADGSVIREGWSSELDGLRSLRSDAHGVL
                     EAYLEEERRSSGLSGLRVRYNKIIGYYLELSKGAAQSAPAHFIRRQSTTNTERFTTER
                     LGQLESDIHGAAERIVELERSLFSALCEELKPSAPLLFETAAELAELDCAASFAWVAT
                     ARGYVRPTVDESLVLEVRGGRHPVVEAYLPSGEFVPNDLLLDGDGTGFALITGPNMAG
                     KSTFLRQNALIALLAQAGSFVPASSARIGVVDRIFCRVGAQDNLARGESTFLLEMHET
                     ASILNNAGARSLVVMDEVGRGTGTLDGLSIAWAVSEHVLDACRSRTLFATHYHELTAL
                     RHERLVDLSMTVEEREGEVVFLKRIRPGAATGSYGIHVARLAGVPLAVVARAMEIKAT
                     LEAGEARLAVDDIPEVGGATLPREPEAGGLFSVEELVLDELRSLDLDRITPIEALSRL
                     AAAQKALRGR"
     gene            5632..6390
                     /locus_tag="PLQ29_02530"
     CDS             5632..6390
                     /locus_tag="PLQ29_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPG85550.1"
                     /translation="MVRPDRVGYASLRALRRAVSTFAPMRCVVCGLPADPDSRYPLCA
                     RCAGRLLDEALESAELSRPAARDPSRCPVCGKLAVSELGPCMRCRSAGYRFDSALPLL
                     RYSGDARAALLSYKAGRTSLASFFADALAVALSGHYSERTVVPVPPRPGKIRRYGWDQ
                     VERIARILERRHGIAVARVLKRAGGHQQKSLGLEARAVNMRGAISLRRGAEAPLAPVL
                     LDDVMTTGATLSACADALKGAGAGRVDAIVIAAD"
     gene            6522..6968
                     /locus_tag="PLQ29_02535"
     CDS             6522..6968
                     /locus_tag="PLQ29_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85551.1"
                     /translation="MTESIELYNDIASLLSGIGVELVELHLSGTHGGTKASAVVYKPG
                     GTGIDECSAAHRLIQARLEVLLGNDDFQLETASPGVDRVIRSPAEYAIFVGRGVRLFL
                     DDEDVVEGRIASSDGEAVVVSTAGGDRSVAIASIRKGKLDYSQEGR"
     gene            6984..>7369
                     /locus_tag="PLQ29_02540"
     CDS             6984..>7369
                     /locus_tag="PLQ29_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HPG85552.1"
                     /translation="MAEAIRQLIYEKGISEDLVLKTIEDALLAAYKKRYGTAENAVVR
                     FTGEYEGVEILAKRTIVDELDDPVLEISLDEAKELVDGAELGDILEIPIDPREFDLQE
                     VMLAKQTTRQSLREISRDTLYAEYKA"
BASE COUNT         1296 a         2385 c         2380 g         1308 t
ORIGIN      
        1 ggtctccgac gtgcgtcgcg agtcggcggc gagcgacaag ggcgcgatgc gcctgaccct
       61 gatcttcgtg atcgacgagg gccgacgcta tacctacggc ggtatgggct tcgccggcaa
      121 ctcgctgtac acgagcgagc agctcggcgc cctcgtccgg cagcagccag gcgccgtgct
      181 caactacgag cgcctgctcc aggaccagtc gcgcgcagcc gacctctact tcgagaatgg
      241 ctatattttc aacggcttca ggctcgctga gacccgcgac gaggaactcg gctccatcgc
      301 gtacacgctc gtgatcgacg agaaaccgca ggcccacatc gaggacgtga tcttccgcgg
      361 caacgtgaag acgaaggaat tcgtgctacg ccggctcgtg cctctcgagt cgggcgacgt
      421 gttctccaag gccaaggtgc tcgagagtct gcgcaacctg tacaataccc agtacttctc
      481 ttcgatagtg cctgaatacg agcagggtag ccaggatctc ttcgtcgacc ttatcatcag
      541 cgtcgaggag cagagcaccg cgtcgatcca gttcggcatg acctactcgc caggcacgag
      601 cgcaggcgag ttcccgatca tcggcctggt caactggagc gacatcaatt tcctcggtaa
      661 cgggcagacc gttgcgctca agaccaacct ggcggtcgac tcgcaggacc tgaccttctc
      721 gttcagcgac gactggctgt tcggcaagcg cctgtccgga tcgctcgagt tcgcgttcga
      781 gcacgagtac ctggaggccg cccaggactc gaccggcgtc atcttcccct acggcgaccc
      841 gacgagggta cccgacccgt acaacagcta cgaagaatac gtggcggcgg gcaagctcgt
      901 gccggacggg tacaagatga cctacgatac ctggaccttc agcctcggct actcgggcgg
      961 ctaccgctgg aataccaagc tgggtaccgc cggcgtcgtg cccggggtta cccacgagct
     1021 ggggatgaag acctacgatt ccgatctgta ccggccctac gactcgacta tctcggagaa
     1081 cctggacacc tggctgttct ccaacacggt ctacgcccgc gcctacctga ataagctcga
     1141 cctatggtac gacccgtcga gaggctacta cgccagccag cgcctcggcc tgaccggttg
     1201 gttcgacggg gagatcaacc acttcctgcg tagcgatacc cgggcggacg ccttcgtcac
     1261 gttgttcgac ctgcccatca ccgaaagcta ttccttcaag ggcgtactcg gcgcccattc
     1321 gaagttctcg gcgctgatcg cccagccctg gtacgacgac ggcctcgtcg cctcggactc
     1381 taagaaactc aacatcgacg gtaccttcgt cggccgcggc tggtcgtcgc tcggctcgta
     1441 ctacggcacc gccctctggg acaactggct cgagttccgt atgccgatcg tgccgagcgt
     1501 gctatcgctc gatggcttcc tgagcgcggc gatgctgtcg accgagagcg gcctcctcga
     1561 cgttgctggt gtcgactcgg gctccacggc ctacggcggg accctgtcgg agctcggtct
     1621 caacaacttc gcgttcagcg ccggcgtcgg cctgcgcttc atcatcctgc agttcccgtt
     1681 caggatctac ttcgccaaga aatttatcta cgacgacggc ttcgcctggt cgtccgacga
     1741 cgactggcag ttcgtcctca gcgtgacgac gagcctcgat tgacgcgcat agcggggggt
     1801 acgatatgaa gaagacggcg atcatcggcc tggcgctatt cgcgctctgc gcccaggcct
     1861 tcggccagca gatcacgcgt gtgggcgtga tagaccttca gaaggtctat acgacctact
     1921 ataaggactc gcaggcggta cgcgcgctgg aggaggagaa ggccagggtc aacgaggaga
     1981 tcaagcgcct gagcgaggag atcaaggagc tgcagaagcg taggctcggg ctcctgtcgt
     2041 ccgccgacgc ggccgcgctc aagaccttcg acgaggagct gtaccgcaag gcccagttcc
     2101 tgtctgactt cgtcaagatc aagcaagctg acctcgacgc caaggccgag agcatgacca
     2161 agggcgacgc gttcgtgcag atgctctata ggaccgtgca ggccatctcg gaggccgagg
     2221 gtttctcgct ggtcattagc tcaaggaacg cgtccgacgt cggctcgtcg gtgatctggt
     2281 tcagccctat gatagacatc accgacaagg tgatacaggc gctcctaggc gcttgagcgg
     2341 cggccttcag tcctgatccg cccccgcgac gcccttgagc gcgtcgcgga gcgcggagaa
     2401 tcggcgctcg ttctcctcgg atagctcaat cagctcctcg tgggccctga gcacgaggcc
     2461 ggcgcccgcc ttggcctcgg cgccaagcct ctcgagcggc tcggggaagg ccggcggctc
     2521 cttctctatc gtcacgagcc tgacgatgcc tatcgtcttg agtagcttca tacaggtctc
     2581 gctcaggccc gcgagccgta tcggccgccc cgagaagcgc atgaagcgct tattgaagcc
     2641 gacgatcagt cccataaagg tcgagtccat gtactcgcac gacgaaaggt cgatgatcac
     2701 atcctcgacc gccggcttgg cgtcgagccg gtcgaacacg cgcgccttga gctccgggca
     2761 ggcgttggcc gttatgtggc cctgagcgcg aatatagacg cggttgttcg tctccttgat
     2821 gtaaaggacc tcttccacga cgacctcgca acgatgaaga tggcgctgcc tctcgcctaa
     2881 cagaatcgcc tcgattgtat cctagtccaa gttaccctat aatgcaacgc catggccgaa
     2941 caggtaccgc tcctcgagca atacgatcgc atcaaggccc tccataggga cgagatactc
     3001 ttcttcaggc tgggcgactt ctacgagatg ttctacggcg acgcggtcga ggcgagctcc
     3061 ctgctcgggc tgacgctgac gcatcgtcag gaagccccga tgtgcgggat acctcatcac
     3121 gcggctcgcg gctatatagc caggctgcta cgggccggca agaagatagc cgtctgcgag
     3181 cagctatcgg ctcccggcag gggcaagggc gtcatcgagc gcggcgtcgt cgaggtggtc
     3241 acgccgggta gcgtggtcga ggacgactat ctggagtcgg gggcgaataa ctacatcgtc
     3301 gccatagggg ccttcggcga cgatatggcc atcgcctggg ccgacgcttc gaccggcgag
     3361 ttcaaggcag gctcgttcgg gcgcggcgat cctgagcggc tgcgcaggga gctgttcagg
     3421 ctgtcgccgc gcgaagccct cgtccgggag tcgaccctcg acgacgacgg cgtggcgcgc
     3481 gccctatccg agcgaccggc tatcgtgctg aaccgattcc cggactggac cttctcgctc
     3541 gagcagggca ggtcgaccct gctcggccac ttcggcacga gcagcatgaa gggcttcggc
     3601 ttcgacgacg gcgacccggc gctggccgcg gctggcgccc tgctcgagta cctgcgcgat
     3661 tcggtcaagg cgcgtctgtc gcagttcagg accctcggcg gttgcgacga cgccgagttc
     3721 gtgtcgatcg acgagtcgag ccagaagaac ctcgagttag accgtaacct gcgcgacggg
     3781 ggggcggcgt tcacgctgta cggcgtcgtc aaccacacga agacggctat gggcgctcgg
     3841 gcgctccgct cgaggctgca gcgcccgctc cggtcggccg ccgcggtcaa cgggcggctc
     3901 gacgccgtcg acgcgctgta tcgcgaccag cgatcgctcg agcgcgtacg cgcgatactc
     3961 ggttcgtgcc gcgacctcga acgcctggcc tcgaggctgg cgatggaccg cgcctcgccc
     4021 aaggatctcg tcgctctgcg ggacacgatc cgggccgcgc tgtcgctggg gccggccctg
     4081 ccgatagccg cgccgaccgc cctgtcgacg ctcgccgaac caggcgatag agacgcggcc
     4141 ctggccttcg ccgacgtcat agcgcgggcc gtggtcgacg acccggccat gtcccccgcc
     4201 gacggctccg tgatacggga gggctggagc tccgagctcg acggtctacg gtcgttgcgg
     4261 tcggacgccc acggcgtgct cgaggcctac ctcgaggaag agcggcggtc ctcggggctt
     4321 tcggggctgc gggtcaggta caacaagatc ataggctact acctggaact gtccaaaggc
     4381 gctgcccagt cggctccggc gcacttcatc cgacggcagt cgacgacgaa caccgagcgc
     4441 ttcacgaccg agcggctcgg ccagctggag tcggatatcc acggcgcggc cgagcgtatc
     4501 gtcgagctgg agagatcgct cttctccgct ctgtgcgagg agctcaagcc gtccgcgccg
     4561 ctgttattcg agacggccgc cgagctggcc gagctggact gcgccgcgtc gttcgcctgg
     4621 gtcgctacgg cccgaggcta cgtccgccct accgtcgacg aatcgctggt tctcgaggtc
     4681 cggggagggc ggcatcccgt cgtcgaggcg tacctgccct cgggcgagtt cgtaccgaac
     4741 gacctactcc tcgatggcga cggaacaggc ttcgccctaa tcaccggccc gaacatggcc
     4801 ggtaagtcga cgttcctcag gcagaacgcg ctcatcgcgc tactcgcgca ggcgggctcc
     4861 ttcgtgcccg cctcgtctgc caggataggc gtcgtcgaca ggatcttctg ccgcgtcggc
     4921 gcccaggaca acctggccag gggcgagtct accttccttc tcgagatgca cgagacggcg
     4981 agcattctga acaacgccgg cgcgcgtagc ctcgtcgtga tggacgaggt ggggcgcggc
     5041 accggcacgc tggacggcct gtcgatagcc tgggccgtca gcgagcacgt cctcgacgcg
     5101 tgccgttcga ggaccctgtt cgccacgcac tatcacgagt tgacggcgct caggcacgag
     5161 cggctcgtcg atctgtcgat gaccgtggag gagcgcgagg gcgaggtggt cttcctcaag
     5221 cggatccggc ccggcgcggc gaccgggtca tacggcatcc acgtggccag gctggccggg
     5281 gtgccgctcg cggtcgtcgc ccgggccatg gagatcaagg cgacgctcga agccggcgag
     5341 gccaggctcg ccgtcgacga tatacccgag gtcggcggcg ctacgctccc gagggaaccg
     5401 gaggccggcg ggcttttctc ggtcgaggaa ctcgtactcg atgagttgcg atcgctggac
     5461 ctggaccgta tcacgccgat cgaggccctt tccaggctgg cggccgccca gaaagccttg
     5521 cgggggcgct gaagcggatg catgagggaa ccggaacgag agccgtaacg gggccggaag
     5581 gggaaccgta acgggtgaat gcgccgtgaa ccttcgcggc gtacaatagg catggtacga
     5641 cctgatcgag ttgggtacgc gtccctccga gcgctccggc gagccgtctc gacgttcgcg
     5701 ccgatgcgtt gcgtcgtctg cggcttgcct gccgaccccg atagccgcta tccgctgtgc
     5761 gcccgatgcg cggggcggct actcgacgaa gcgctcgagt cggcggagct ttcacggcca
     5821 gccgcccgag atccttcgcg ctgccccgtc tgcggcaagc tcgccgtatc ggagctcggc
     5881 ccttgcatgc gctgccggtc ggctggctac cgattcgact cggccctgcc gctcctgcgc
     5941 tattccggag atgcgcgggc cgcgctcctg tcgtataaag ctggacgcac gagcctcgcc
     6001 tcgttcttcg ccgacgcctt ggccgtggcc ctatcggggc attactcaga gagaacggtc
     6061 gtacccgtgc cgcctcggcc cggcaagata cgacgttacg gctgggatca ggtcgaacgc
     6121 atcgcccgta tactggagag acgccacggg atcgccgtag ccagggtgct caagcgcgcg
     6181 ggcggccacc agcagaagag ccttggcctc gaggctcgcg cggtgaacat gcgcggcgcc
     6241 atatcgctac ggcgcggagc ggaagcgcct ctcgcgccag tcctgctcga cgacgtgatg
     6301 acgaccggcg cgaccctgtc cgcctgcgcc gacgcgctca agggcgccgg cgcggggcgt
     6361 gtcgacgcca tcgtcatagc ggccgactga tagatggcca tggcgagggg cctgcggcgt
     6421 attgacttag cgtgacgcga aggctatgat ggctacagac atggtttttc ttagtttgaa
     6481 tgtaagaaga gtcgttccca cggctctttt tttataggtg tatgacggag tcaatagaac
     6541 tctacaatga tatagcctct ctcctctcgg ggattggcgt cgagctggtc gagttgcacc
     6601 tgagcggtac gcacggcggt acgaaggcgt ccgccgtcgt ctacaagccg ggcggcacgg
     6661 gcatcgacga gtgctccgcg gcgcaccgtc tcattcaggc tcgtctggaa gtcctgctcg
     6721 ggaacgacga cttccagctg gagaccgcct ccccaggcgt cgaccgcgta atccgctctc
     6781 cggccgagta tgccatcttc gtcggccgtg gcgttcgtct attcctcgac gatgaggacg
     6841 tcgtcgaggg gcgtatcgcg tcttcggacg gcgaggcagt cgtcgtctcg accgcgggcg
     6901 gagaccgttc agtcgctatc gcaagcattc gaaaaggtaa gctcgattat tcccaggagg
     6961 gtaggtaagc catggcctca gatttggcag aagcgattcg ccagctcata tatgaaaagg
     7021 gtatctcgga ggatctagtc ctcaagacga tcgaggacgc gttgctggcg gcgtacaaga
     7081 agcgctatgg caccgcggag aacgccgtcg tccgcttcac cggcgagtac gagggcgtcg
     7141 agattctcgc caagcgcacc atcgtcgacg aactggacga tccggtactc gagatatcgc
     7201 tggacgaggc caaggagctg gtcgacggcg ccgaactcgg ggatatcctc gagattccga
     7261 tcgacccgag ggagttcgac ctgcaggagg tgatgctcgc caagcagacg acccgtcagt
     7321 cgctccgcga gatatcccgc gacactctgt acgccgagta caaggccaa
//
LOCUS       DAOPHV010000056         7239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_13185_length_7239_cov_4.387389, whole genome shotgun sequence.
ACCESSION   DAOPHV010000056 DAOPHV010000000
VERSION     DAOPHV010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13185_length_7239_cov_4.387389"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..946)
                     /gene="thyX"
                     /locus_tag="PLQ29_02545"
     CDS             complement(116..946)
                     /gene="thyX"
                     /locus_tag="PLQ29_02545"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPG85553.1"
                     /translation="MAHCIVDEAEKVLDAEFKVHDKGFVRLVDYLGGDARIVQSARVS
                     YGDGTKTVREDAGLIDYLLRNEHTSPFEQVVLTFHIKMPIFVARQWIRHRTARVNEMS
                     GRYSVMKDEFWIPAPEAVARQSADNKQGREVDPLSPDEAARVIGLLEEGQRSAYASYK
                     AMVDSGLARELARVNLPLSLYTEWYWQIDLHNLFRFLALRLDAHAQREIRDYAAVILD
                     MTRKVCPLACASFERHLLAGVRLSAEEASAFRRLLDGGEAGLGGRELERLEEKLAKVR
                     "
     gene            complement(983..1876)
                     /locus_tag="PLQ29_02550"
     CDS             complement(983..1876)
                     /locus_tag="PLQ29_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPG85554.1"
                     /translation="MRADLHIHSRFSDGGHWPSDIAGLAAAAGLEAVCLTDHDTFGGC
                     VELAEAAGQLGLASWPAVEIDCEDETLGYKSELLAYFPGGSYELTASMLAVSRAERAA
                     RVEAMFARAAALFAAPELSFGRWVEARMAGRGPGSPRVDLGTLRFAKTDLFLALKRAG
                     RVSADVDYREFKKAYFDTGLFSDLRFAKPSLETVAESVSRDGGTLVVPHIGHEFGDSI
                     ASMRAGTRRLDAMLSRFRDLGVRGVELYYYRNDDREAMNALVTERCERFGFFHTYGSD
                     CHGPDSNKNGLGSFYGDFRGF"
     gene            1960..2871
                     /locus_tag="PLQ29_02555"
     CDS             1960..2871
                     /locus_tag="PLQ29_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HPG85555.1"
                     /translation="MFDLDDAIIEELLFSMENQDIDFMVDATTGAVVVMNDDALDSGL
                     DSLDEGADLLPPPDWTSADGYGLMEAFAAGVGDPIARTSLAAALSRGRGVFKAFKTAI
                     EAYPDIARRWYDYKRCAMAKRVAEWYDEARVARGLERLGPEPEDDDLLADDFSFRIAG
                     REAWLECQPLFRRGLEEALGAFPEPLVEYEYTAIEREVAEGGKEGLVIVLAEAVAGAI
                     AGVAAARRVYVADGSFGKLTYLYVAPEHRRLGLGRRLAEAARERLAKDGIPRFVVDMP
                     FVPAGFGSSMKAFGYEPFGTRYLKTDD"
     gene            complement(2909..3220)
                     /locus_tag="PLQ29_02560"
     CDS             complement(2909..3220)
                     /locus_tag="PLQ29_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPG85556.1"
                     /translation="METIGDRIKATLRLVPPGRAVSYGAVAALAGAPNGARTVARILH
                     SSAAAEGLPWWRVVRADGGIALRRGYGFEEQEARLVDEGVEVGRDGRVDLGRYGWDGA
                     R"
     gene            3344..4264
                     /locus_tag="PLQ29_02565"
     CDS             3344..4264
                     /locus_tag="PLQ29_02565"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPG85557.1"
                     /translation="MKTCILFPGQGAQFVGMGRDLYDTDAETRALFEEASDVARLDLA
                     ALMFEGDEDELKRTRNAQLALALADAAAYAALRKRGVSADGVAGFSLGEWAALCAAGV
                     LPFGRMIALVAERGRLMDQACARSGGSTMSAVLGLEAASIEAALTGAGVTDAWLANLN
                     APTQSVLSGTETGIAKAEAAVKEAGAKRVVRLKVAGAFHSPLMRYAYDGFKEIIADES
                     FADPTIAFYSNVTGGRVSTGTELKELAAEQIIRPVAWTAIERAMAADGYGRELEAGPG
                     SVLAGLWKAAGLEPPVTPAFSADQIAALEP"
     gene            4275..5009
                     /gene="fabG"
                     /locus_tag="PLQ29_02570"
     CDS             4275..5009
                     /gene="fabG"
                     /locus_tag="PLQ29_02570"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPG85558.1"
                     /translation="MEHGMLSGRRCLVTGGSKGIGKAVVEAFLREGAAVDYLSRSKAD
                     DHDSLAAMGPLGWLACDVSDAEALEAVVEEAVKTAPYDVVVNNAGVTRDGLSFRMPLD
                     DWRAVIDTNLTSAFIVSRVAARAMIKRRSGSVINVASVVGLVGNGGQTNYSASKAGLI
                     GLTKSLAREVASRGVRVNAIAPGYIDTAMTEAIPETAKSALRERIPLGRTGAPAEVAS
                     VALFLASDQASYVTGEVIRIDGGMAM"
     gene            5136..>7239
                     /gene="glgX"
                     /locus_tag="PLQ29_02575"
     CDS             5136..>7239
                     /gene="glgX"
                     /locus_tag="PLQ29_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HPG85559.1"
                     /translation="MVRPGHPRPFGATPVPGGVNFALFSRHATSVRLCLFAKADDGEP
                     ILDHRLDPVQNRTGDIWHIHVDGLRPGALYAWRADGPFMPERGYRFNPNKLLVDPYAK
                     ALSGDFEWDMKAALGYDADDPRADLSFSRIDDAATMPKCVVVDDDEFDWEGDKPLNLP
                     LRHCVIYETHVRGLSAHASAGSSHPGTYLGLVEAIPYLKRLGVTSLELLPVQEFDHHE
                     NTRKDPRTGKRLSQYWGYSTLAFFAPKGRYASSSRSGQQVAEFKSMVKALHRAGIEVI
                     LDVVFNHTGEGSELGPTVSFRGLDNPVWYMLDEDPRRYKNYSGCGNTLNCNHPVVRGF
                     IVDCLHYWVADMHVDGFRFDLGSILGRDQKGRLLENPPVIEQIAEDPLLRQTKIIAEA
                     WDAGGAYQVGGFPGGRWAEWNDRYRDDVRRFWRGDEGAVAAIATRLTGSSDLYLRDGR
                     KPFHSINYITSHDGFTLRDLVSYNGKHNERNGERGLDGHDSNFSYNHGHEGPSRAPAV
                     ESLRVRQQKNFLATLLLSIGTPMLLGGDEFGRTQRGNNNAYCQDNETGWYDWSLAVEH
                     DELLRFTARLIALRLAHPAFRRPEFFTGKDANYNALPDIAWYAPDGSAPDWASMPSAL
                     AARLDGSKADIVADRDDDDFLLLLNATAGAIDFRVGAPQAGWAWYRAVDTNLSSPYDA
                     AEPGCEPPVDHETPYRLGP"
BASE COUNT         1264 a         2506 c         2340 g         1129 t
ORIGIN      
        1 ggtccgcgtc agcccgcggg cctcgatgac gttctctggc atttcgtatc ctcctctatg
       61 atagaggagg atacggcgtt ggcagcgggt cggtgcacta ctggagggtt tccttttagc
      121 gaactttggc cagcttctct tccaggcgct cgagctcgcg gcctccgagc cccgcctcgc
      181 cgccgtcgag cagccgcctg aaggctgacg cctcctcggc cgacagcctg actccagcga
      241 gcaggtgccg ttcgaagctg gcgcaggcga ggggacagac cttccgcgtc atatcgagga
      301 tcaccgccgc atagtcgcgg atctcccgtt gcgcgtgggc gtccagcctc agcgccagga
      361 agcggaacag gttgtgcagg tctatctgcc agtaccactc ggtgtacagc gacaggggca
      421 ggttgacgcg ggccagttct cgggccaggc ccgagtcgac catcgctttg tatgaggcat
      481 aggccgacct ctgtccctcc tcgagcaggc cgatgacgcg agcggcctcg tccggggaca
      541 gaggatcgac ctcgcggccc tgcttattat cggcgctctg cctggcgacc gcctcgggag
      601 ccggaatcca gaattcatcc ttcatcaccg agtagcggcc ggacatctcg ttcacgcgcg
      661 cggtacggtg gcgtatccac tggcgcgcga cgaagatcgg catcttgata tggaaggtca
      721 gcacgacctg ctcgaacggc gaggtatgct cattacgcaa caggtagtcg ataagacccg
      781 cgtcctcgcg cacggtcttc gtgccgtcgc cgtatgacac gcgggccgac tggactatcc
      841 tcgcgtcccc gcccaggtag tccaccagcc gcacgaagcc cttgtcgtgt accttgaatt
      901 cggcgtcgag taccttttcc gcctcgtcga cgatgcagtg cgccattgtc ttgtcctttc
      961 ggctaatcgc ggcccgctag acctagaatc ccctaaagtc gccgtagaac gaaccgagcc
     1021 cgttcttgtt cgaatccggc ccgtggcaat cggagccgta ggtgtggaag aagccgaagc
     1081 gctcgcagcg ctcggtgacc aacgcgttca tcgcctcccg gtcgtcgttc cgatagtagt
     1141 acagctcgac gccgcgtacg ccaaggtccc ggaagcgcga cagcatggcg tccagccgtc
     1201 tcgtgccggc gcgcatcgac gcgatcgagt cgccgaactc gtgcccgata tgcggtacga
     1261 cgagcgtgcc gccgtccctg gagaccgact cggccaccgt ctcgaggctc ggcttggcga
     1321 agcgcaggtc ggagaacaac ccggtatcga agtaggcctt tttaaattct cgatagtcta
     1381 cgtcggccga cacgcggccg gcccgcttga gcgccaggaa taggtccgtc ttggcgaagc
     1441 gcagcgtgcc gaggtcgaca cgcggcgacc cggggccgcg gccggccatg cgcgcctcga
     1501 cccagcgccc gaacgacagc tccggagccg cgaacagggc ggcggcgcgg gcgaacatag
     1561 cctcgacgcg cgcggcccgc tcagctcgcg acacagcgag catcgaggcc gtgagctcgt
     1621 aggaaccgcc cgggaaatag gcgagcagct cgctcttata gccaagcgtc tcgtcctcgc
     1681 aatcaatctc gacggcgggc caactggcca gaccaagctg tccggccgcc tcggccagtt
     1741 cgacgcagcc gccgaaggtg tcgtgatcgg tcaggcagac ggcctcgagt cccgccgcgg
     1801 cggcgagccc ggctatgtcc gacggccaat ggccgccgtc ggagaagcgc gaatgtatat
     1861 ggagatcggc tcgcatcgtg gtaccctggg tagtatacta acccgcgagg agcgcggcgg
     1921 acaagccgac gaacgccgcg cccccgaccg gaggacagaa tgttcgacct agacgacgcg
     1981 atcatagagg agctgctgtt ctcgatggag aaccaggaca tagatttcat ggtggacgcc
     2041 acgaccggcg ccgtcgtcgt gatgaacgac gacgcgctcg actcgggcct ggactcgctc
     2101 gacgagggcg cggacctgct accaccgccg gactggacca gcgccgacgg ctacggactt
     2161 atggaggcct tcgcggccgg cgtcggagac ccgatagcca ggacgagtct cgccgccgcc
     2221 ctatcgcgag ggcgaggggt cttcaaggcc ttcaagacgg cgatcgaggc ctaccccgac
     2281 atcgcgcggc gctggtacga ctataagcgc tgcgcgatgg ctaagcgcgt cgccgagtgg
     2341 tacgacgagg cgagggtggc ccgcggcctc gagcgcctcg gcccggagcc cgaggacgac
     2401 gacctcttgg ccgacgactt ctcgttcagg attgccggcc gcgaggcgtg gctcgaatgc
     2461 cagccactat tcagacgcgg cctcgaggaa gcgctcggcg cgttcccgga gcccctggtc
     2521 gagtacgagt acaccgccat cgagcgggag gtagccgagg gcggcaagga ggggctcgtg
     2581 atcgtgctag ccgaggccgt ggcgggggcg atagccggcg tggccgcggc gcgacgggtc
     2641 tacgtggccg atggttcctt cggcaagcta acctacctgt acgtggcgcc ggagcaccgc
     2701 aggcttggcc tcggacgtcg cctggccgag gccgcacgag agcgcctggc caaggacggc
     2761 ataccgcgct tcgtggtaga catgcccttc gtgccggccg gcttcggctc ctcgatgaag
     2821 gccttcggct acgagccctt cggcacgcga tacctgaaga ccgacgactg acggccaccc
     2881 ctcgctgacg acgggctcag cgagagccct agcgcgcgcc gtcccagccg taacggccga
     2941 gatccacccg gccatcgcga cctacctcca cgccctcgtc gaccagccgc gcctcctgct
     3001 cctcgaagcc gtagccgcgg cgtagcgcta tgccgccgtc ggcgcgtacg acgcgccacc
     3061 agggcaggcc ctcggccgcg gcgctcgaat gcagtatccg tgcgaccgtg cgcgccccgt
     3121 tcggcgcacc cgccagcgcg gccaccgcgc cgtagctcac ggctcggccc ggaggtacga
     3181 gccgcaatgt agcctttatc ctgtcgccga tcgtttccat gagcgccagc ataccacagg
     3241 tacggcggcg cgcccgcccc gcgcgggcga tcatgagagg tccattgttg atcttgacat
     3301 attgacacat attgtctcag tatcgagcga ggagctacac cagatgaaga cctgcatcct
     3361 gtttcccggg cagggcgccc agttcgtcgg catgggcagg gacctgtacg atacggacgc
     3421 agaaacacgc gccctgttcg aagaagcgtc agacgtcgcc aggctggatc tcgccgcact
     3481 gatgttcgag ggcgacgagg acgagctcaa gcgcacccgc aacgcccagc tggccctggc
     3541 gctcgccgac gcggccgcct acgccgcgct acgcaaacgc ggcgtctcgg ccgatggcgt
     3601 cgccggcttc agcctcggcg aatgggcggc tctctgcgcc gcgggggttc tgcccttcgg
     3661 ccgcatgata gctctcgtcg ccgagcgcgg ccgcctgatg gaccaggcct gcgcccgctc
     3721 tggcggctcg acgatgagcg ccgtgctcgg gctcgaggcc gcctcgatcg aggccgcgct
     3781 cacgggagcg ggtgtaaccg acgcgtggct cgccaacctc aacgcgccga ctcaatcggt
     3841 gctgtcgggc acggagaccg gcatcgccaa ggcggaagcg gccgtcaagg aggcgggcgc
     3901 caagcgcgtg gtaaggctca aggtagccgg cgccttccac tcgcctctga tgcgctacgc
     3961 ctacgacgga ttcaaggaaa tcatagccga cgagagcttc gcagacccga cgatcgcctt
     4021 ctactcgaac gtgaccggag gcagagtctc gacggggacg gagcttaagg aattagccgc
     4081 cgagcagatt atccgaccgg tggcctggac ggctatcgag cgcgcgatgg cggccgacgg
     4141 ctacggacgc gagctggaag ccgggccggg ctcggtcctg gctgggctct ggaaggcggc
     4201 cgggctcgag ccgccggtaa cgccggcgtt ctcggccgat cagatagcgg cgctggagcc
     4261 gtaggagggc cacgatggaa cacggaatgc tgagcggtag gcgctgcctc gtcacgggag
     4321 gctccaaggg cataggcaag gccgtcgtcg aggccttcct gcgcgagggc gcggcggtag
     4381 actacctgtc gaggtccaag gccgacgacc acgactcgct ggcggccatg ggaccgctcg
     4441 ggtggctggc ctgcgacgtg tcggatgccg aggctctcga ggccgtcgtc gaggaggcgg
     4501 tcaagaccgc gccgtacgac gtggtcgtca acaacgccgg cgtcacacgc gacgggctgt
     4561 cgttcaggat gccgctcgac gactggcgcg ccgtgatcga tacgaacctg acgagcgcct
     4621 tcatcgtctc gagggtggcc gccagggcca tgatcaagcg ccgctccggt agcgtcatca
     4681 acgtggccag cgtcgtcggc ctcgtcggca atggcggcca gacgaactac tccgccagca
     4741 aggccggcct catcggcctt accaagagcc tcgcccgcga ggtcgcgagc cgcggcgtcc
     4801 gcgtcaacgc gatagcgcct ggctacatcg acaccgcgat gactgaggcc attcccgaga
     4861 ctgccaagtc cgccctgcgc gagcgcatac cgctcggcag gacgggcgcc ccagcggaag
     4921 tcgcgtccgt ggcgctattc ctggcctcgg accaggcctc ctacgtaacc ggcgaggtga
     4981 tcaggatcga cggcgggatg gcgatgtagc gccggagtac gccggtgata cataagattc
     5041 gtaacactat aggcggcgtg ctattctcca ctcatcggat tatactggta gcctgaaccg
     5101 acgagccgga ggacagcgtg ggcacacccg aatacatcgt acgtcccggg cacccgcgcc
     5161 ccttcggggc cacgcccgtc ccgggcggcg tcaacttcgc gctgttctcg cgtcacgcga
     5221 cgagcgtacg cctctgcctc ttcgccaagg ccgacgacgg ggagcccatc ctcgaccatc
     5281 gcctggaccc ggtccagaac cgcaccggcg acatctggca catccacgtc gacggcctgc
     5341 ggcccggcgc cctctatgcg tggcgggccg acggtccctt catgccggaa cgcggctatc
     5401 gcttcaaccc taacaagcta ctcgtcgacc cctacgccaa ggcgctgtcc ggtgacttcg
     5461 agtgggacat gaaggcggcc cttggctatg acgcggacga cccgcgcgcc gacctgtcct
     5521 tttccaggat cgacgacgcg gccacgatgc ccaaatgcgt cgtggtcgac gacgacgagt
     5581 tcgactggga gggcgacaag ccgctcaacc tgcccctgcg ccactgcgtg atctacgaga
     5641 cccatgtacg cggcctgtcc gcccacgcga gcgccggctc gagccacccg ggcacctact
     5701 tgggcctcgt cgaggccatc ccctacttaa aacgcctcgg cgtgaccagt ctcgagttgt
     5761 tgccggtcca ggaattcgac caccacgaaa acactcgaaa ggatccgcgc acgggcaagc
     5821 ggctatccca gtactggggc tacagcaccc tcgccttctt cgcgcccaag ggccgctacg
     5881 cctcgtcgag ccgctccggc cagcaggtgg ccgagttcaa gtccatggtc aaggcactgc
     5941 accgagccgg catcgaggtg atactcgacg tggtgttcaa ccataccggc gagggtagcg
     6001 agctcggccc gaccgtgtcc ttccgcggcc tcgacaaccc ggtctggtac atgctcgacg
     6061 aggatccgcg ccgctacaag aactactcgg gctgcggcaa cacgctgaac tgcaaccatc
     6121 ccgtggtccg aggcttcatc gtcgattgtc tccactactg ggtcgcggac atgcacgtcg
     6181 acggcttccg tttcgacctc ggctcgatac tcgggcgcga tcagaagggc cgcctgctcg
     6241 agaacccccc ggtcatcgag cagatagccg aggacccgct actcaggcag accaagatca
     6301 tcgcggaagc ctgggacgcg ggcggcgcct atcaggtcgg cggctttccg ggagggcgct
     6361 gggccgagtg gaacgaccgc taccgagacg acgtgcggcg cttctggcgc ggcgacgaag
     6421 gcgccgtggc cgccatcgcc accaggctaa ccggctcgag cgacctctat ctgcgcgatg
     6481 gccgtaagcc gttccattcg atcaactaca tcaccagtca cgacggcttc accctgcgcg
     6541 acctggtcag ctacaacggc aagcacaacg agcggaacgg cgagcgcggc ctcgacggcc
     6601 acgactccaa cttcagctac aaccacggcc acgagggccc gagccgcgcc ccggccgtcg
     6661 aatcgctgcg cgtgcgccag cagaagaact tcctggccac actgctcctg tccatcggca
     6721 ccccgatgct gctcggcggc gacgagttcg gcaggaccca gcgcggcaat aacaacgcct
     6781 actgccagga taacgaaacc ggctggtacg actggagcct ggccgtcgag cacgatgagc
     6841 tgctacgctt cacggccagg ctcatagccc tccggctagc ccatccggcg ttcaggcgcc
     6901 ccgagttctt caccggcaag gacgccaact acaacgccct gccggacatc gcctggtacg
     6961 cgcctgacgg ttccgcgccc gactgggctt ctatgccgtc ggccctcgcc gctcgcctcg
     7021 acggctccaa agccgatatt gtcgccgacc gcgacgacga cgacttcctt ctcctcctga
     7081 acgcgacggc cggcgccatc gacttccgcg tcggcgcgcc gcaggcgggc tgggcctggt
     7141 accgcgccgt cgacacgaac ctgtcgtcac cctacgacgc ggcggaaccg ggatgcgagc
     7201 cgcccgtaga ccatgaaacg ccctatcgcc tcggcccca
//
LOCUS       DAOPHV010000057         7222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_13234_length_7222_cov_4.637784, whole genome shotgun sequence.
ACCESSION   DAOPHV010000057 DAOPHV010000000
VERSION     DAOPHV010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13234_length_7222_cov_4.637784"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2316)
                     /locus_tag="PLQ29_02580"
     CDS             complement(<1..2316)
                     /locus_tag="PLQ29_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85560.1"
                     /translation="MGSVPAASAILAPLLAFLAARWLALLVGKRYPNGRVGTMAALGV
                     VSSVALSAIPTIRRLEAGAYWLAADAASVAAMGALGATLGARLGVVAKAPGRAVPSDE
                     PAAWRRGLALALPLGVGFAIAAVFGLPTGADGQAVARAAVAFAATLWLAVDARPPALA
                     LAGVALVFAQFRSPAITRPPIQPVVAAIATIAAIAAAAATYKARPARDTAPALASSSP
                     SAQAAEAFQAFAAVALAWSLTAAGLGWPAAGLVAGAVGSLAGRRARHASSASGTGRFK
                     IESAMALAFIAGYGVSAPKAAANLPAALVIAASILVATALVSVLARNGLSRVPVAAGA
                     FAAMLVARRSGLASDAAMAGLVLAYLVAIPVSARASARPGTDDTARRIGTIVGVRPGA
                     DNLAAMALGAALDGRDRPVMAACVAAPEGSPGLGVDQAEETLVRCVAAAASTDVRVVP
                     TLVASRTVAAGLAGLASERGAELLLVGFDDEQNPSGTVDGLLASFPGAVIAVKRPGEF
                     QAAKRLVALAVSGAEASPGFGRAIAAIARAWAGDPKSVEAVMIGAPASAMLEAAEGLL
                     RPSRASSVASWRDVPAAIGTPTSDEAAFVVFSSRPGAKDWNPGHERLPVVMSSSFPDS
                     PTLLWFPPTASDDADKGDDAPAPALPTEPVAASEPYDRLPTIVQAAYAAGRVFVDMGE
                     DSLVDAIRRLTDSIFPFDRVASGRLAAQFSTVARKEPIELAPGILLLHAHAPGVSQTT
                     LAIGSRPDGWPIIALSSPVRVAVALVSPDDSG"
     gene            complement(2382..3311)
                     /gene="murB"
                     /locus_tag="PLQ29_02585"
     CDS             complement(2382..3311)
                     /gene="murB"
                     /locus_tag="PLQ29_02585"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPG85561.1"
                     /translation="MSTLRKFLEKCNIDAEIAYEEPMAAHTSFRIGGPAEAFVKPRTA
                     KATAALLAAARDFGVPVATVGGGANLLVADAGVRGVVLSTELLRGLSRRDDGSLYAEA
                     GLPVVDLVRSAIRDGEAGLEFAAGLPGSVGGAVYMNARCYDREFADALLGIAYLDPLT
                     LEPATATVERDAWAYKRTPFMPGGALAGALILGARFRLTHGDRAALEARAAELEADRA
                     TKGHFDYPCAGSVFKNDRSFGRPTGKILDELGFRGRRVGDAMVSPKHANIFVNAGRAT
                     ASDMSVLIAQAIAEAKAAFGIELEPEVVTLGDF"
     gene            3562..5100
                     /gene="cysS"
                     /locus_tag="PLQ29_02590"
     CDS             3562..5100
                     /gene="cysS"
                     /locus_tag="PLQ29_02590"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPG85562.1"
                     /translation="MGLRLYNTLGRSLQEFEPIQPGKVGFYGCGPTVYNYAHIGNMRM
                     YVFEDTLARALRYLGYEVTHVMNITDVGHLSGDADSGEDKMVKSAQERHKSVLEIARY
                     YTDAFFADEAKLNILRPDVVCNATEHVDDMISLIRRLEANGYTYFAGGNLYFDVSKFP
                     SYGEMAGLKLDELKAGARIEVDANKRNPYDFVLWFTKSKFEDQALQWDSPWGRGYPGW
                     HVECSAMSMKYLGEQFDIHAGGIDHVAIHHTNEIAQSEGATGKKWVNYWLHGEFLVLD
                     KGKMSKSAGGFLTLKTLTDAGYEALDYRYFLLGAHYRSQLQFSYDGLDSARASRRSLV
                     ERVLALREKLHGGEPTPLATLCPAAKARLVAFKAAIEDDLSTPRALAELWGVLKEQGV
                     SAADALGAAFDMDRVLGLGLESLAREAQAAVDPALAAELDALVAERAVAKKAKDYKRA
                     DAIRDELKARGVILEDSPAGTSWKLAPSVAGATPSVAGATPSVTGATPSVAGATPPGS
                     APRA"
     gene            5117..5656
                     /locus_tag="PLQ29_02595"
     CDS             5117..5656
                     /locus_tag="PLQ29_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPG85563.1"
                     /translation="MFDDRGGVDDASFKALYDETFTMLWRVARRITNSDEAAEDVVHD
                     SYIKAVERWSIFPTRDDAKYWLIRVVKNNALNWAKRKGREQKVYQRYFKETERVSESP
                     EAEVVRNESKDDMRRYLDRLPEKFRVVLVLKEYGLLNYKEIGKSLGIAEGNVKVRVFR
                     AREALAKMMREDGTDVSRP"
     gene            5640..6215
                     /locus_tag="PLQ29_02600"
     CDS             5640..6215
                     /locus_tag="PLQ29_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPG85564.1"
                     /translation="MCPDRELLSAWIDGEVPSPWSEAIERHLDGCASCAEAARSLRLV
                     GERLRAESAGIDAAAESAAARVYDRVAPSLRPSAVAGNAVPKASSALWARRLAVPVPA
                     AVAAALAIAAMAFALVTSGRRNAEMRMAMERAYEATPVASAGIESVIDYLSRQNSAVN
                     INITLPPEAFGGSVGDPFIVREADYRGGSGR"
     gene            6212..6751
                     /locus_tag="PLQ29_02605"
     CDS             6212..6751
                     /locus_tag="PLQ29_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85565.1"
                     /translation="MSRRLMAAMAAAGFVASASWAQAGPEPPAPVAAQARESGSLSSI
                     PTAMLGEALTVSIVASIGQPSEAPAWQASDVKYTIPGSAVSVKMIGRDAVIVITVTPY
                     RSKDGGLLLVAQGQVWYKEGDAGLRYRTTVDTLSVRFGERVLFYPFGAHPETGAPLRV
                     ELVMDEYAPGDGTAAEGNP"
     gene            6748..>7222
                     /locus_tag="PLQ29_02610"
     CDS             6748..>7222
                     /locus_tag="PLQ29_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HPG85566.1"
                     /translation="MRAGFSPPRPKRRGGSTVIAVTAVALALAAIVVVPIARDRASRP
                     VVSSIRPAIGDPGGVLTIEGDNFGPLRDEGRVEFDGTAPTASSYLSWSDDRIEVRVPL
                     YADSSLVRVVTPSGRSNATMFMSRALLPTSPSGGGSTSLGPIIESLSSESGAIGSA"
BASE COUNT         1210 a         2431 c         2494 g         1087 t
ORIGIN      
        1 acccgagtcg tccggggaca cgagggccac ggcgacgcgg accggggacg acagggcgat
       61 gatgggccag ccgtccggcc gagagccgat cgccagggtc gtttgcgaga cgcccggcgc
      121 gtgggcgtgc aggagcagga tgccaggagc cagctctatc ggctccttgc gcgcgacggt
      181 ggagaactgg gcggccagcc tgcccgaggc gacccggtcg aacgggaaga tcgagtcggt
      241 caggcgacga atagcgtcga ccagcgagtc ctcgcccatg tcgacgaaga ccctgccggc
      301 cgcgtaggcg gcctggacta tggtcggcag ccggtcgtac ggctcagagg cggccacggg
      361 ctcagtcggt agtgccggag ccggtgcatc gtcgcccttg tccgcgtcgt cgctcgccgt
      421 gggcgggaac cagagcaacg tcggcgaatc ggggaacgac gagctcatga cgactggtag
      481 gcgttcgtgt ccagggttcc agtccttggc gccggggcgg gatgagaaca cgacgaaggc
      541 cgcctcgtcg gaggtcggcg tgccgatagc cgctggaacg tcgcgccacg aggcgaccga
      601 cgaggctcgg gacggtcgaa gcaggccctc ggcggcctcg agcatggcgc tggcaggcgc
      661 gccgatcatg accgcctcga cggacttggg gtcgccggcc caggctcgcg cgatggccgc
      721 tatagcgcgg ccgaaacctg gagacgcctc ggccccggag acggccaggg cgacgaggcg
      781 cttggccgcc tggaactcgc ccggccgctt gactgcgatg acggcgcccg ggaacgaggc
      841 gagcagtccg tcgaccgtgc cggacgggtt ctgctcatca tcgaagccga cgagcaggag
      901 ctcggcccct cgctcgctgg cgaggccggc taatcctgcg gcgacggtgc gcgacgcgac
      961 gagggtcgga acgacgcgca cgtcggtcga ggccgccgcc gccacgcatc ggacgagcgt
     1021 ctcctccgcc tggtcgacgc caagcccagg cgagccctcg ggcgcggcca cgcacgcggc
     1081 catgacgggg cgatcgcgcc cgtcgagggc cgcgcccagg gccatggcgg cgaggttgtc
     1141 cgctccgggg cgcacgccga cgatggtccc gatgcgccta gcggtatcat ctgtccccgg
     1201 acgcgccgag gctcgcgccg atacgggaat ggcgacgagg tacgccagta ccaggccggc
     1261 catcgccgcg tcgctggcca gcccggaccg cctggcgacc agcatggccg cgaaggctcc
     1321 ggcggccacc ggaacacgcg atagcccatt ccgggccagg accgagacca gggccgtcgc
     1381 gacgagtatc gaggcggcta tgaccagggc cgccggcagg ttcgccgcgg ctttcggggc
     1441 ggaaacgccg tagccggcta tgaacgcgag cgccatggcg gactcgatct tgaaacgccc
     1501 cgttccggag gcggaactag cgtgtcgcgc gcgcctgccc gccagactgc cgaccgcccc
     1561 ggccacgagg ccggccgccg gccaacccag tcccgccgct gtgagcgacc aagccagggc
     1621 caccgcggcg aaggcctgga aggcctcggc cgcctgggcc gacgggctgg aggatgcaag
     1681 agccggcgcc gtatcgcggg ccggcctcgc cttgtaggtc gccgcggccg ccgctatggc
     1741 agctatcgtg gcgatagctg cgacgaccgg ttggataggg ggccgggtta tggccggcga
     1801 cctgaactga gcgaacacca gcgctacgcc ggcgagcgcc agcgccggcg gccgagcgtc
     1861 gacggcgagc catagcgtcg ccgcgaaggc gacggccgcc ctggccacgg cctggccgtc
     1921 ggcaccggtt ggcaggccga ataccgccgc tatcgcgaat ccgacgccaa ggggcagggc
     1981 gagcgcgagg ccacgtctcc aggccgcggg ctcgtcactc ggaacggcgc gtccgggagc
     2041 cttggctacc acgccgaggc gcgcgccgag cgtcgctccc agggcgccca tggcggctac
     2101 ggaagctgcg tcggccgcca gccagtaggc gccggcttcg agcctccgta tcgtagggat
     2161 ggccgacagc gccaccgatg agaccacgcc gagcgccgcc atcgtcccga cccgtccatt
     2221 cgggtatcgc ttgccgacga ggagcgcgag ccagcgcgcg gccaggaagg ccaggagagg
     2281 cgctagtata gccgaggccg ccgggacgct acccatatcg taggactaaa actaggcgat
     2341 ccctcgcccc ggcgcaaggg tcgatcggac gcgggtcggc cctagaagtc gccgagcgtt
     2401 accacctcgg gttcgagctc gataccgaac gccgccttcg cctcggctat cgcctgggct
     2461 atcagcacgc tcatatccga ggccgtggcg cggccggcgt tcacgaagat gttcgcgtgc
     2521 ttcgggctga ccatcgcgtc gccgacccgg cgccccctga agccgagctc atcgagtatc
     2581 ttgccggtcg gacggccgaa ggaccggtcg ttcttgaaga cgctcccggc gcagggatag
     2641 tcgaagtggc ccttcgtcgc cctgtcggcc tcgagctcgg cggcccgagc ctcgagggcg
     2701 gcgcggtccc cgtgggtcag cctaaaacgg gcgccaagga tcaaggcgcc ggccagcgcg
     2761 ccgccgggca tgaagggcgt gcgcttgtag gcccaggcgt cgcgctcgac ggtcgcggtc
     2821 gccggctcga gcgtgagcgg atcgaggtag gcgatcccga ggagggcgtc ggcgaattct
     2881 cggtcgtagc agcgggcgtt catgtacacg gcgccgccga ccgagcctgg caggccggcg
     2941 gcgaactcga gtccggcctc tccatcgcgt atcgccgacc gcaccaggtc gacgacgggt
     3001 agcccggcct cggcgtacag ggagccatcg tcgcgccggc tcaggccccg gagcaactcg
     3061 gtggacagca cgacgccgcg cacgccagcg tccgccacga gtagattggc gccgccgcct
     3121 accgtggcga ccggaacccc gaagtcgcga gcggccgcga gcagcgccgc cgtcgccttg
     3181 gcagtcctcg gcttgacgaa ggcctcggcc ggcccgccta tgcggaagct cgtatgcgcg
     3241 gccatcggct cttcgtaggc tatttccgcg tctatattgc atttctccag gaatttccgt
     3301 agtgtactca tgctgttcgt atggtatccc accctgcgaa tagtgttaag atcaagatct
     3361 atacgtttat accacggagg cacggagaca cggaggaggg aagagagaaa gagtagagga
     3421 tggatgccgt cctatgagac tcactaactt tcttaaccaa tcttcttgtt cttgtcctct
     3481 ccgtgtctct gtgtctccgt ggttcaaatg ccgttttatt gtatgagttc tgttcgacgc
     3541 atatcttgaa aggaggaccg catgggtttg cgactctaca acacgctcgg acgttccctg
     3601 caggagttcg agccgataca gccggggaag gtaggcttct acggatgcgg cccgaccgtc
     3661 tataactatg cgcacatcgg caacatgcgc atgtacgtct tcgaggatac gctcgcccgc
     3721 gcgctccgct acctcggcta cgaggttacg cacgtgatga acatcaccga cgtcggccac
     3781 ctgtctggcg acgcggactc gggcgaggat aagatggtca agagcgccca ggagcggcac
     3841 aagtccgttc tcgagatagc gcggtattat accgacgcct tcttcgccga cgaggccaag
     3901 ctcaatatcc tcagacccga cgtcgtctgc aacgccaccg agcacgtgga cgacatgata
     3961 agcctgatac ggcggctcga ggccaacggc tacacctatt tcgccggcgg caacctctac
     4021 ttcgacgtgt ccaagttccc gagctacggc gagatggccg gcctcaagct cgacgagctc
     4081 aaggcgggag cgcgcataga ggtcgacgcc aacaagcgca atccctacga cttcgtcctc
     4141 tggttcacga agtccaagtt cgaggaccag gcgctgcagt gggatagccc ctgggggcgc
     4201 ggctaccccg gctggcacgt cgagtgttcc gcgatgagca tgaagtactt aggggagcag
     4261 ttcgacatac acgccggcgg catagaccac gtggccatcc accacacgaa cgagatagcc
     4321 cagagcgagg gagccaccgg caagaagtgg gtcaactact ggctccacgg cgagttcctc
     4381 gtcttggaca agggcaagat gtccaagtcg gcgggcggat tcctcacgct caagaccctg
     4441 accgacgcgg gctacgaggc tctcgactac cgctacttcc tcctgggcgc ccactaccgg
     4501 tcccagcttc agttctccta cgacgggttg gattccgccc gggcttcgcg gcgttccctg
     4561 gtcgagcgcg tactcgcctt gcgcgagaag ctgcacggcg gggagccgac gccgctcgcg
     4621 acgctgtgcc cggccgccaa ggccaggctc gtcgccttca aggctgccat agaggacgat
     4681 ctgtccacgc cacgggctct cgccgagctc tggggcgtcc tcaaggaaca gggcgtgagc
     4741 gcagccgacg ccctgggcgc agccttcgac atggaccgcg tgctcgggct cgggctcgag
     4801 agcctcgcgc gcgaggccca ggccgccgta gacccggctc tggccgccga gctggacgcc
     4861 ctggtcgccg agcgcgccgt cgccaagaaa gccaaggatt acaagcgcgc cgacgccata
     4921 cgagacgagc tcaaggcgcg cggcgtcatc ctcgaggata gcccggcggg cacctcctgg
     4981 aaactcgcgc cgtcagtagc cggcgcgacg ccgtcagtag ccggcgcgac gccgtcagta
     5041 accggcgcga caccgtcagt agccggcgcg acgccgccgg gaagcgcccc cagggcgtaa
     5101 ctgactggag gctggattgt ttgacgatag gggcggcgtg gacgacgcgt ccttcaaggc
     5161 cttgtacgac gagaccttca cgatgctctg gagggtagcc aggcggataa ccaactccga
     5221 cgaggccgcc gaggacgtcg tgcacgactc gtacatcaag gccgtcgagc gctggtcgat
     5281 cttcccgacc agggacgacg ccaagtactg gctcatccgc gtcgtcaaga acaacgccct
     5341 gaactgggcc aagcggaagg gtagggagca gaaggtctac cagcgctatt tcaaggagac
     5401 ggagcgagtc tccgagtcgc ccgaggcgga ggtcgtcagg aacgagtcga aggacgacat
     5461 gaggcgatac ctcgaccgcc tgccggagaa gttcagggtc gtgctcgtgc tcaaggagta
     5521 cggcctgctt aattacaaag agatcggcaa gtctctcggc atcgccgagg gcaacgtgaa
     5581 ggtgcgcgta ttcagggcgc gagaggcgct cgcgaagatg atgcgggagg atggtaccga
     5641 tgtgtccaga ccgtgagctc ttgtccgcct ggatcgatgg cgaggttcct tcgccatgga
     5701 gcgaggccat cgaacgccat ctggacggtt gcgcttcctg tgccgaggcc gcgcggtcgc
     5761 tacgcctcgt aggcgagcgc ttgcgcgccg aatcggccgg tatcgacgcc gccgccgagt
     5821 cggcggccgc ccgcgtctac gatcgcgtcg cgccgagcct gcgtccgtcg gccgtagccg
     5881 gcaacgcggt tccgaaggct tcgtccgccc tctgggcgcg ccgcctagcg gtgcccgtcc
     5941 ccgccgcggt cgccgcggcc ctggcgatag cggcgatggc cttcgctctc gtgacgagcg
     6001 ggcgtcgtaa cgccgagatg cgcatggcca tggagagggc ctacgaggct acgccagtgg
     6061 ccagcgcggg catcgagtcg gtgatcgact acctgtccag gcagaattct gccgtcaata
     6121 tcaatatcac cctacctccc gaagccttcg gcgggtcggt cggcgatccg ttcatcgtgc
     6181 gcgaggctga ttaccggggc gggagcggcc ggtgagtcgt cgactgatgg ccgccatggc
     6241 ggcggcgggc ttcgtggcct cggcatcttg ggcgcaggcc ggccccgagc cgccggcccc
     6301 ggtcgccgcc caggcgcggg agtcgggttc cctatcgtcg ataccgacgg ccatgctcgg
     6361 cgaggccctg accgtctcca tcgtcgcgtc cataggccag ccttccgagg ccccggcctg
     6421 gcaggcgagc gacgtcaagt acaccattcc gggtagcgcc gtgtcggtca agatgatagg
     6481 ccgcgacgcg gtcatcgtca tcacggtcac gccgtacagg agcaaggacg gcgggctcct
     6541 actcgtggct cagggccagg tctggtacaa ggaaggcgat gccggactgc gctatcggac
     6601 caccgtggac acgctgtcgg tacgcttcgg cgagcgggtg ctgttctacc ccttcggcgc
     6661 ccatccggag accggcgccc cgctacgcgt ggagctcgtg atggacgagt acgcacccgg
     6721 cgacggaacg gcggccgagg gtaatccttg agggccggct tctcgcctcc gcgcccgaaa
     6781 cgacgcggag gctccaccgt catcgccgtc accgcggtag ccctggcgtt ggcggccatc
     6841 gtcgtcgtgc cgatagccag ggacagggcc tcgaggcccg tcgtgtcgtc gatacgtccg
     6901 gccataggcg acccgggcgg ggtgctgacc atcgagggtg acaacttcgg cccgctccgc
     6961 gacgaaggca gggtcgagtt cgatggtacc gcgccgaccg cgtcctcgta cctttcatgg
     7021 agcgacgatc gcatcgaggt gcgcgtgccg ctctacgccg actcgtccct ggtccgcgtc
     7081 gtcacgccgt ccggtaggtc gaacgccaca atgttcatga gccgggccct gttgcccacc
     7141 tcgccttcgg gcggcggctc gacctcgctc ggccccatca tcgagtccct gtcctccgag
     7201 tccggggcca tcggctcggc cc
//
LOCUS       DAOPHV010000058         7190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_13314_length_7190_cov_4.237842, whole genome shotgun sequence.
ACCESSION   DAOPHV010000058 DAOPHV010000000
VERSION     DAOPHV010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7190
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13314_length_7190_cov_4.237842"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="PLQ29_02615"
     CDS             <1..930
                     /locus_tag="PLQ29_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85567.1"
                     /translation="RIIERLMAPFMKPLFGLPGVASLAAVLTFLSDNPAILSLASDKG
                     YASYFKKHQLYSLTNFGTAFGMGLIVITYMMGLGFYREAFVGLAGAVVGAIVSTRLMQ
                     GRVKRRLGVVDVVSPEDQVKFREDNVLRAQDQGLVVRVINALLDGGKSGVQAGFSIIP
                     GVLIISTAVMMITWGPKDAAIGYQGLAYEGVPILPALGRLLSPLFRLLFGFESPEAIA
                     FPITSLGAVGAALGLVPRFMAQGLAHGNDIAVFTAMGMCWSGYLSTHTGMMDAMGRRD
                     LIGDALLSHTVGGLVAGVAAHYVYLGVRLLGWA"
     gene            complement(962..2362)
                     /locus_tag="PLQ29_02620"
     CDS             complement(962..2362)
                     /locus_tag="PLQ29_02620"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HPG85568.1"
                     /translation="MTLIRGGLVVDGTGAPARRADVLVDDERIAAIGAVPKAPPGAAV
                     IDATGAVVAPGFIDAHTHDDVAALDGVNLAKLTQGITTVVVGNCGFSLAPWTPSLRRP
                     TPPEPLGILGGPERFTYPDFASYLAALGPDGVALCGHSSLRALRVADLGRPASPDELA
                     AMVVDLETALDSGCAGLSAGLAYPAAIAASTEEVTALARVAFRRGKAFAIHLRNEYDG
                     VWDALDEAFSIARSALPGSPEPGARLVLSHQKCAGLRQRGRAGELLERIERAARELPV
                     SFDAYPYEAGSTMLEAESVRASSRVLVTWSEPHPELAGLELAEAAERLGLDERAALEA
                     LKPGGGAYFHMDEADVDAILAHPLCMVGSDGLPADAVPHPRLYGAFPRYLSRMVREKG
                     LLGLEEAVRKVTSLPAAVFSLGSKGLLRVGADADVVVFDPERFRDRADWREPRRYCEG
                     IRTALRRGTLERLPGG"
     gene            complement(2359..>2832)
                     /locus_tag="PLQ29_02625"
     CDS             complement(2359..>2832)
                     /locus_tag="PLQ29_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85569.1"
                     /translation="RYDACDGVVGDSETGGDAGRYDACDGVVGDSETGGDADRYDTCD
                     GGATDRDNACDGGAVMRRAKAIFELDLARIEATDIFFYLLDGRVPDEGAAVALGIAYA
                     DKAARRPDRLLLGLHTDGRSAFPDAALNPMLAAALDRVFTDERSLLAYLEGLPRR"
     assembly_gap    2833..2932
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2933..3133)
                     /locus_tag="PLQ29_02630"
     CDS             complement(<2933..3133)
                     /locus_tag="PLQ29_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85570.1"
                     /translation="MLDNKSDDRLNPEGRSMRFYFAAPLFCDAERRYNADLAGKVEAL
                     GYDVFLPQRDGVVGDSETGGDAG"
     gene            3202..3615
                     /locus_tag="PLQ29_02635"
     CDS             3202..3615
                     /locus_tag="PLQ29_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85571.1"
                     /translation="MKRVTTSVCASIVLASVCLLCSCDGLFGPSIPSELHGEWSWTIA
                     HYYERFTITATTVSYFGQSTEYAGYAASWSEEILEVHEADRILEITGDGNGPMYLPWH
                     LSGNTLYLDTTNPGDAYPILADEWWLGSGYKLSRE"
     gene            complement(3640..4092)
                     /locus_tag="PLQ29_02640"
     CDS             complement(3640..4092)
                     /locus_tag="PLQ29_02640"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPG85572.1"
                     /translation="MLEEGSIAPDFTLDDDSGKPVSLSSFRGRKVVLYFYPKDDTSGC
                     TKEACGFRDAYGSFIERGAVVVGVSPDSSGSHARFKAKYGLPFVLLADPEHRALEAYG
                     VWAEKSMYGRKYLGVVRSTYVIGEDGAILRAFPKVSPEGHAAEILAAL"
     gene            complement(4121..6985)
                     /locus_tag="PLQ29_02645"
     CDS             complement(4121..6985)
                     /locus_tag="PLQ29_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPG85573.1"
                     /translation="MDRVHTARPTVIVSSLGLALAGAALFVSLVAASLGAADGFGGFL
                     LDGLSWAAYALPAYPWLGAVIIAMPGFRYDLLIGLLLSLLPFASLAATARFLDDPTHA
                     LASYPAIAPLGILGVAAVGLCLTLGSGALVCLAVVRARAGAVGSAETEAGVDAGPAPT
                     GRRVSWRGSRDDARAERETSLKPELEAPLKRARSAHVAEQAEPPVGTAADGFETRAFD
                     LSYPDLPPVPFLASMAAPEPESVAAESADPSPTITSGSEDVAAMSDDDAAGDDSAAGD
                     DSAAGDDDAAGDDDAAGDDEPELLVDLDDVRPQADEPIPLMFGRTESKAAAQVGLVAS
                     DPALDADFWANAGLVDAEPAEGEPAAADRRASLLPRVEVFQDGEHAEALRSNGEAKRD
                     FDPAAVTPAATLVKVARPVPEETSVPPAKPAPAPPEGEPAASKPRLPGRKPVVAYSMP
                     TEGILTAYPDGQYWIIDDKTRKAAEILRETLQEFGIEAEVTGIRKGPVITMFEILPAP
                     GVRLSKIANLCDNIALRLAASSVRIVAPIPGKHAVGIEVPNERRAIVSFKEIVESETF
                     KNAKMEVPVVLGKDITGDVQVVELTHTPHLLIAGATGSGKSVCVNSIILSILYRRSFN
                     EVKLILIDPKIVELKLYNGIPHLLTPVITEPKRAFQALQYCICEMERRYSLLDAMGVR
                     DIKSYNKKIVEKSMATDKLPYIVVIIDEFADLMATTGKELESTLARLAAMSRAVGIHL
                     VLATQRPSIDVITGLIKANIPSRIAFMVAGKFDSRIIIDMVGAEKLLGRGDMLFSGST
                     DAFPVRMQGAFVSDDEVERVVAHVKTLGEPDYIDEEIFVDDEDDEGPSLFDDEDDDPL
                     YEKALEIVLSQGKASASFLQRKLKIGYNRAARIVELMEEKGIVGKAQGSKPRDLVHNP
                     DMGSRPPNPRARYGPDFSADDDGDDDADMASDGEAEASEN"
BASE COUNT         1128 a         2369 c         2416 g         1177 t
ORIGIN      
        1 cgcatcatcg agcgcttgat ggctccgttc atgaagccgc tgttcggact gccgggcgtc
       61 gcgtcgctcg cggcggtgct gaccttcctg tccgataatc cggccatcct gagcctggct
      121 agcgacaaag gctacgcgag ctacttcaag aagcaccagc tctactcgct gaccaacttc
      181 gggacggcct tcggcatggg cctcatcgtc atcacctata tgatgggcct cggcttctac
      241 cgagaggcct tcgtcggctt ggccggagcg gtcgtcggcg ccatcgtatc gacgaggctc
      301 atgcagggcc gggtcaagcg caggctcggc gtcgtcgacg tcgtgagccc agaggaccag
      361 gtcaagttcc gcgaggacaa cgtgctgcgc gcccaggacc agggactcgt ggtacgcgtc
      421 atcaacgcgc tgctcgacgg cggcaagtcg ggcgtacagg ccggcttctc catcataccc
      481 ggcgtactga tcatctcgac cgccgtgatg atgataacct ggggaccgaa ggacgccgcg
      541 attggctacc agggccttgc ctacgagggc gtgccgatcc taccggccct cgggcgcctc
      601 ctatccccgc tcttcaggct actgttcggc ttcgagagcc ccgaggccat cgcgttcccg
      661 attacgagcc tcggcgctgt cggcgcggcg ctcggcctcg tgccgcgctt catggcccag
      721 ggcttggccc atggcaacga catagcggtc ttcacggcga tgggcatgtg ttggtcgggc
      781 tacctgtcga cccacaccgg catgatggac gcgatgggac gccgcgacct gataggcgac
      841 gcgctcctct cgcacaccgt gggcggcctc gtcgcgggcg tcgccgcgca ctacgtctac
      901 ctgggcgtcc gcctgctcgg ctgggcctag gccggggctt ctcggcgcgc tcagctggcg
      961 ttcagccgcc cggaagacgc tccagcgtac cgcggcgtag cgccgtgcgg atcccttcgc
     1021 agtaccgtct tggctcgcgc caatcggctc ggtcccggaa ccgttccggg tcgaacacga
     1081 cgacgtccgc gtcggcccca acccgcagta ggccctttga gccgagcgag aagacggcgg
     1141 cgggcaggga cgtgaccttg cgtaccgcct cctcgagccc gagcaggccc ttctcgcgta
     1201 ccatgcggct gagatagcgc ggaaacgcgc cgtagagcct cggatgcggc actgcgtcgg
     1261 ccgggaggcc gtcggaaccg accatgcaga ggggatgggc caggatggcg tcgacgtctg
     1321 cctcatccat gtggaagtaa gccccgccgc cgggcttcag agcctcgagg gcggcacgct
     1381 cgtccagccc gagccgttcg gcggcctccg cgagctcaag accggccaac tcgggatggg
     1441 gctccgacca ggtgacgagc acgcggctcg aggcccgtac cgactcggcc tcgagcatcg
     1501 tcgagccagc ctcgtaggga taggcgtcga acgagacggg cagctcgcgc gcggcgcgct
     1561 ctatccgttc gagcagctcg cccgcccggc cgcgttgccg gagtccggcg catttctggt
     1621 gcgacaagac gaggcgcgcg ccgggctcgg gcgagcctgg caaggctgac ctggctatcg
     1681 agaaggcctc gtcgagggcg tcccagacgc cgtcgtactc gttgcgcagg tggatagcga
     1741 aagccttgcc gcgacggaag gccacgcgcg cgagggccgt cacctcctcg gtcgacgccg
     1801 ctatggccgc cggataggcg agccccgcgg acagcccggc gcagcccgag tcgagcgccg
     1861 tttcgaggtc gacgaccatg gccgccagct cgtcgggcga cgccggacgg cccaggtcag
     1921 ccacgcgcag ggcccgtagc gacgaatggc cgcacagcgc cacgccgtcc gggccgagcg
     1981 ccgccaggta cgaggcgaaa tccggatagg tgaagcgctc cggcccgccc aggatgccga
     2041 gcggttcggg cggcgtcggg cgccgaaggc tcggcgtcca cggcgccagc gagaagccgc
     2101 agttgcctac caccacggtc gtgatcccct gcgtcagctt ggccaggtta acgccgtcga
     2161 gcgccgctac gtcgtcatgg gtgtgcgcgt cgatgaagcc aggcgccacg acggccccgg
     2221 tggcgtcgat tacggctgct ccaggcggag ccttcggcac ggcgcctatc gccgcgattc
     2281 gttcgtcgtc gaccagcacg tcggcgcgcc gagcgggcgc cccggtgcca tcgactacca
     2341 gccctcctcg tatgagcgtc atcggcgcgg cagcccttcg aggtaggcca ggagcgatcg
     2401 ctcgtcggta aagacgcggt cgagtgccgc ggcgagcatc gggttcaggg cggcgtcagg
     2461 gaaggcagag cggccgtcgg tatggagccc gaggagcagc cgatccggcc gtcgcgccgc
     2521 cttgtcggcg taggcgatgc cgagagcgac cgctgcccct tcgtcgggca cccggccgtc
     2581 aagcaggtag aagaatatgt cggtagcctc gatgcgtgct aggtcaagct cgaagatggc
     2641 cttggcccga cgcatgacgg cgccgccgtc gcatgcgtta tctcggtccg tggcgccgcc
     2701 gtcgcatgtg tcgtagcggt ccgcgtcgcc gcccgtctcg gaatctccga ccacgccgtc
     2761 gcatgcgtcg tagcggcccg cgtcgccgcc cgtctcggaa tctccgacca cgccgtcgca
     2821 tgcgtcgtag cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcccgcgt
     2941 cgccgcccgt ctcggaatct ccgaccacgc cgtcgcgctg cggcaggaac acgtcgtaac
     3001 cgagggcttc caccttcccg gcgaggtcgg cattatagcg ccgctcggca tcgcagaaca
     3061 ggggagcggc gaaatagaag cgcatggatc gtccttccgg attaagcctg tcgtcgctct
     3121 tgttatcgag catatgcgga ttatactgaa ttgttagaac tacacctaca tcccgacaac
     3181 agtacacgtt aaggagtccg tatgaaacgc gtgacgactt ctgtctgtgc gtcgatcgtc
     3241 ctggcgtccg tctgcctgct ctgttcctgc gacgggctct tcggtccgag catcccgagc
     3301 gagctgcatg gcgaatggag ctggaccatc gcccattact acgagcgctt caccatcacg
     3361 gccacgacgg tcagctactt cggccagtcc acggagtacg cagggtacgc cgcgtcttgg
     3421 agcgaagaga ttctcgaggt gcacgaggcg gatcggatcc tcgagataac tggagacggc
     3481 aatggtccca tgtacctgcc ctggcacctg tcgggcaata cgctctacct ggatacaacc
     3541 aaccccggcg acgcttaccc tatcctcgct gacgaatggt ggctcggctc gggctataag
     3601 ctgtcccgag aatagcggga gggcggcccg acggccggat caaagcgcgg ccaggatctc
     3661 ggccgcgtgg ccctcgggcg acaccttggg aaaggccctc agtatagcgc cgtcctcgcc
     3721 gatgacatag gtcgagcgga cgacgcccag gtatttccgg ccgtacatcg acttctcggc
     3781 ccagacgccg taggcctcga gcgcgcggtg ttccgggtcg gccaacaaga cgaagggcag
     3841 gccgtacttg gccttgaagc gagcgtggga gccgctcgag tccgggctga cgccgacgac
     3901 caccgcgccc cgctcgatga atgagccgta ggcgtcgcgg aagccgcagg cttccttggt
     3961 gcagcccgac gtgtcgtcct tggggtagaa gtacagcacg accttccggc ccctgaacga
     4021 cgataggctc acgggcttcc ccgagtcgtc gtcgagcgtg aaatcaggcg cgatcgatcc
     4081 ttcttcgagc aaaacggact cctttcgccg aagcgtcgcc tcagttctcg gaggcctcgg
     4141 cctctccgtc cgacgccatg tcggcatcat cgtccccgtc gtcgtccgcg gagaagtcgg
     4201 gcccgtagcg ggcccggggg ttcggcggcc tcgagcccat gtcggggtta tgcacgagat
     4261 cgcggggctt actgccctgg gccttgccga cgatgccctt ctcctccatc agctcgacga
     4321 tacgggcggc gcggttgtac ccaatcttaa gcttgcgctg gaggaagctg gcgctcgcct
     4381 tgccctggga cagcacgatc tcgagggcct tctcgtagag cgggtcgtcg tcctcgtcgt
     4441 cgaacaggga cggcccttcg tcgtcttcgt cgtcgacgaa gatctcctcg tcgatgtagt
     4501 cgggctcgcc gagcgtcttg acgtgggcca cgacgcgctc cacctcgtcg tcggagacga
     4561 aggcgccctg catgcggacc gggaacgcgt cggtggagcc ggagaacagc atgtcgccgc
     4621 ggccgagcag cttctcggcg ccgaccatgt cgatgatgat gcgcgagtcg aacttgccgg
     4681 cgaccatgaa cgcgatgcgg ctcgggatgt tcgccttgat caggcccgtg atcacgtcga
     4741 tgctcggccg ctgggtggcg agcacgaggt ggatgccgac ggcgcggctc atcgccgcga
     4801 gcctggccag ggtcgactcg agctccttgc cggtggtggc catcaggtcc gcgaactcgt
     4861 cgatgatcac gacgatgtac ggcagcttgt cggtggccat gctcttctcg acgatcttct
     4921 tgttgtagct cttgatgtcg cgcacgccca tggcgtcgag caggctgtag cgcctctcca
     4981 tctcgcagat gcagtactgc agggcctgga acgcccgttt cggctcggtg atcaccgggg
     5041 tcaggaggtg gggtatgccg ttgtacagct tgagctcgac gatcttcggg tcgatcagga
     5101 tcagcttgac ctcgttgaac gagcgccggt acaggatgga caggatgatg gagttgacgc
     5161 agacggattt ccccgagccg gtggcgccgg cgatcaggag atgcggggta tgggtcagct
     5221 cgacgacctg aacgtcgccg gtgatatcct tgcccagcac gaccggcacc tccatcttgg
     5281 cgttcttgaa ggtctcgctc tcgacgatct ccttgaacga cacgatggcc cgccgctcgt
     5341 tcggtacctc gatgccgacg gcgtgtttgc ccggtatcgg ggcgacgatg cgcacgctcg
     5401 aggctgccag cctgagcgcg atattgtcgc agaggttggc tatcttggag aggcgcacgc
     5461 ccggggcggg cagtatctcg aacatggtga tgaccgggcc cttgcggatg ccggtcacct
     5521 cggcctcgat gccgaattcc tggagcgtct cgcggaggat ctcggcggcc ttccgcgtct
     5581 tatcgtcgat gatccagtac tggccgtcgg gataggcggt caggatgccc tcggtcggca
     5641 tcgagtaggc tacgaccggc ttgcggccgg gtaggcgcgg cttggaggcc gcgggctcgc
     5701 cttcgggggg cgcgggggcg ggcttggcgg gcgggactga cgtctcctcg ggtacgggcc
     5761 tggctacctt gaccagcgtc gcagccggcg tgacggccgc gggatcgaag tcacgctttg
     5821 cctcgccgtt cgatcgcaac gcctcggcgt gctcgccgtc ctgaaaaacc tcgaccctcg
     5881 gtaacaggga cgcccttcga tccgctgccg ccggctcgcc ttcggcgggc tcggcgtcga
     5941 cgagtccggc gttggcccag aaatcggcgt cgagggcggg atcgctggcg acgagtccga
     6001 cctgggcggc ggctttcgac tcggtccggc cgaacatcag cggtatcggc tcgtcggcct
     6061 ggggacggac gtcatcgagg tcgacgagca actcgggctc atcatcgccc gcggcgtcgt
     6121 cgtcgcccgc ggcgtcgtcg tcgcccgcgg cgctgtcgtc gcccgcggcg ctgtcgtcgc
     6181 ccgcggcgtc gtcgtcgctc atggcggcga cgtcctcaga gccagacgtt atcgtcggcg
     6241 atggatcggc cgactcggcc gctacactct cgggctcggg agcggccatg gacgccagga
     6301 aaggcaccgg aggcaggtcc gggtagctca ggtcgaaggc gcgcgtttca aagccgtcgg
     6361 cggccgtacc gaccggcggc tcggcctgct cggctacgtg ggcggaccgc gcccgtttta
     6421 gtggcgcttc tagctccggt tttagcgacg tttctcgctc cgcccgcgcg tcatcgcgcg
     6481 agccgcgcca ggacacccgc ctgccggtcg gagccggccc ggcgtcgact ccggcctcgg
     6541 tctcggcgga gccgacagcg ccggctcgag cgcgaacgac ggccaggcag accagggcgc
     6601 cggagccgag agtcaagcag aggcctacgg cggcgacccc gaggatgccg agcggcgcaa
     6661 tggccggata cgacgccagg gcgtgcgtcg gatcgtcgag gaaccgggcg gtggcggcga
     6721 gcgaggcgaa cgggagcagg ctcaacagga gccctatgag caggtcgtac ctgaagccgg
     6781 gcatcgcgat gatcacggcg cccagccagg ggtacgccgg caacgcgtac gcggcccagg
     6841 acagaccgtc gagcaggaag cccccgaaac catccgccgc tccgagcgag gccgcgacga
     6901 gcgacacgaa cagcgccgcg cctgcgagcg ctaggccgag cgacgagacg ataacggtcg
     6961 gccttgcagt atgaactcta tccaatgacg tagactccga tccttatgta ccagtatcgg
     7021 ctaaacgtca aaggatacaa agcccgatga gccccgcggg tatgagatac gccgcgaacc
     7081 aggccagctt gcccttcctg accagcggca tgagcgcgag gagggccccg acgccagcgg
     7141 cgaaagcgac caccgaaccg acgaccagct gcgggaccgc gacgctgccc
//
LOCUS       DAOPHV010000059         7120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_13496_length_7120_cov_4.068790, whole genome shotgun sequence.
ACCESSION   DAOPHV010000059 DAOPHV010000000
VERSION     DAOPHV010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13496_length_7120_cov_4.068790"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="PLQ29_02650"
     CDS             <1..1248
                     /locus_tag="PLQ29_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85574.1"
                     /translation="LRPAHRAVVAPLCDSLVEIELEDEGQRVKDAPASSFTATAEDKP
                     RLLLGLLARDEDRPFVVFCNLKESAESVARMLRSRGVKTEYVLGNLQRKRAVLDSVVA
                     GEYEALVLTDEGAAGLPGAWAARLVNWDLPLEGEPYLSRLVHLDASAHGARVYNFACD
                     RYSLGIPAIERALGRRLEPTRADESIMSFAGPVEPTAPEPRARAAETPRPDRRKPEPR
                     RSEVREHRGQQDRRDRVGQYDGRNIKAIQDDIAAITGGKPSATPNRARAQLPAGKGVE
                     QPRRGKKARGTADKTPRQDTTTARREAPDRAGKGVTTPAGRRSRGGSESRIRDPYSIS
                     MEERLRLYRERYGSAGEPKPAGGKPSTAQPSRSSQERKRSEPRTAPVQRAPAVESVPV
                     PPPDPMPRGILGAIKGLFGKKDE"
     gene            1532..1690
                     /locus_tag="PLQ29_02655"
     CDS             1532..1690
                     /locus_tag="PLQ29_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85575.1"
                     /translation="MRMRSALAKYEEAIVGIYNPYVTTITFEETPVGANEMGERIVKN
                     GGRLLARF"
     gene            complement(1687..2646)
                     /locus_tag="PLQ29_02660"
     CDS             complement(1687..2646)
                     /locus_tag="PLQ29_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPG85576.1"
                     /translation="MNDWVFGIDGGGTSSRLRAESMDGAVLYEGSGGSTNLNSNPTEA
                     VTATLETLVSGAFAAGLDPARCRAGFIGSAGVDLDADRETLSGAMRVAFVSAATRAGF
                     AATAVAGARFGAANDAEPALVGALDDVEGFLLIAGTGSIAYGRDRSGRHARAGGLGHF
                     LGDEGSAFWVAFEAIKRGLRSIEGRDEPTGLIEEALAFFGFADAREFIPMAYWDFDKA
                     RIASFAPRVAVLAASGDRLARSIMDQAALELAALVSSVYTRLSPGMIRRRLAPYGGFI
                     QRNAELRSAVTAILRESHPDVEVVDAKGDAQTGACELARGLQT"
     gene            complement(2643..3812)
                     /locus_tag="PLQ29_02665"
     CDS             complement(2643..3812)
                     /locus_tag="PLQ29_02665"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HPG85577.1"
                     /translation="MTDRYYSVGLMSGTSVDGIDAAVSEHVVDGDGGVGSRLVAAIGV
                     PLADSARAAVFSLFEDGAGSLDALALLDMELGEAFAGAALAAIREAGLKPSDVAVIGS
                     HGQTIRHVAGSPGSPRRASLQIGSGAMIAARTGIATVCDFRSGDIAVGGTGAPLVPFF
                     DRIASARFDKPIAFQNYGGIGNVCWIPENGPLVAFDTGPANMIADRLVSEMTDGRLGY
                     DRDGLIGSRGSLRRDLLARWLDHPFLAKAPPKSSGREEFGSAFYESSLRPLLGGVPEG
                     QNREALYADLIRTAETFAAASAARAYRELLPALPRLVVVSGGGARNPVIMAELRAALP
                     ECEVRDADEVGLSVDYKEAMAFGLFGLLRLLGRPNTEPEATGAARAISAGALYLP"
     gene            complement(3809..4729)
                     /gene="murQ"
                     /locus_tag="PLQ29_02670"
     CDS             complement(3809..4729)
                     /gene="murQ"
                     /locus_tag="PLQ29_02670"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HPG85578.1"
                     /translation="MIDLSGLMTERENPASLGIDSKGTREILAIINAEDKKVPFAVEP
                     ALDNIAALVDDVVESFRRGGKLVYIGAGTSGRLGVLDASECPPTYGTPPGMVQGLIAG
                     GREALVRSIESAEDREEMGVKDLDGVGFGPGDVLVGITASGQAPYVLGAMRRARELGA
                     VVGAISCNANSGTFALADHRIFLDVGPEVVTGSTRMKSGTAQKLVLNMITTTAMIRLG
                     KVYRNLMIDLRPVNKKLVERSKNLIMRAAECDRATAERAFAESGRKPKIAILMVLLGI
                     GVDEAERLVAANDGHLSKAVDAYRSTGGGA"
     gene            complement(4726..5715)
                     /locus_tag="PLQ29_02675"
     CDS             complement(4726..5715)
                     /locus_tag="PLQ29_02675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG85579.1"
                     /translation="MTIRAVDPEAVAAELAALWNRSAGTAFPLDERLLRQQVALDTDP
                     RLCLAVARPDGSLVAASLVKRAVRPGADGVAPRIGYLSLLAVDEAERRRGLAAGLLAR
                     SVAWLASRGAERLELGGDYYHLVPGRPVEDGAGFEALDAFLRAVGFEGEGFEYDLSAD
                     LTRAAWLSEPERLPNGSYRYALYEPKYRGALLRFIGSSFPGRWSHEIREALDAGIRGA
                     DLMLAVDERDGSVVGFSRIYDKDSPLLGPGVYWRALMGEHPGGLGPIGVDPSRRGLGL
                     GMGLLEHCVRDLARRGVGTMVIDWTDLVDFYGKLGFKVWKRYERMSLRIRLER"
     gene            complement(5717..6595)
                     /locus_tag="PLQ29_02680"
     CDS             complement(5717..6595)
                     /locus_tag="PLQ29_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPG85580.1"
                     /translation="MTSGVALLREIVDSCTESERKIAEYLIENPKTAVFCTIAELARQ
                     ADTNPPAVIRLCKRAGLSGYRELQILLTRDLYATTDQADEEPPPAFELDSSQSVDAIA
                     RTIVSRTTDAVERVLSLLNTSAVEEAATAILGARRVAIFGVGASANVAYDLYQKLSRI
                     GVPCSFAFDADVQVSIACGLGGSDAAVAVSYSGRSAHTVTIVEEAKRSGARLIGITSV
                     GQSPLSRLADTALYVPATEPLLRTGASLSRATQLVMVDILYATIICRSIEVSIPRLER
                     SLKAVHEQDLKAVEPR"
     gene            6747..>7120
                     /locus_tag="PLQ29_02685"
     CDS             6747..>7120
                     /locus_tag="PLQ29_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPG85581.1"
                     /translation="MKTIANMTLEQKIGQMLLIGYPGGAAGLRTLERVVSRRPMGNII
                     LFSRNAKAPGPLRDELAYVRSMIAGQAGVEPLVSIDQEGGIVTRLGAGFTPLPGAMAL
                     AAAVSGGAATTADIEAMATVNGS"
BASE COUNT         1187 a         2478 c         2359 g         1096 t
ORIGIN      
        1 ctcaggccgg cgcatcgggc ggtcgtcgcc ccgctctgcg actcgctcgt cgagatcgag
       61 ctggaggacg agggccagcg cgtcaaggac gctcccgcct cgagcttcac ggccacggcc
      121 gaggacaagc cgcgcctgct gctcggcctg ctcgcgcgcg acgaggatcg gccgttcgtc
      181 gtcttctgca acctcaagga aagcgccgag tcggtggcgc gcatgctgcg atcgcgcggc
      241 gtcaagaccg agtacgtgct cggcaacctg caacggaaac gcgccgtgct cgactcggtc
      301 gtagccgggg aatacgaggc gctcgtgctg accgacgagg gagccgccgg cctgccgggc
      361 gcctgggccg cgcgcctggt caactgggac ctgccgctcg agggcgagcc atacctctca
      421 cgcctcgtcc atctggacgc gtcggcgcac ggcgcccgcg tctataactt cgcctgcgat
      481 cggtactcgc tcggcatccc ggccatcgag cgggccctgg gccgtcgcct cgaaccgacc
      541 cgtgctgacg agtccatcat gtccttcgcc ggacccgtcg agcctacggc ccccgagccg
      601 cgcgctcgtg ccgccgagac gccccgcccc gatcgccgga agcccgagcc gcgtcgctca
      661 gaggtgcgag agcaccgcgg gcagcaggac cgcagggacc gcgtcggcca gtacgacggc
      721 cggaacatca aggcgatcca ggacgacatc gccgccatca ccggaggcaa gccgtccgcg
      781 acgccgaacc gagcgcgggc gcagctgccg gccggcaagg gcgtcgagca accgagacgc
      841 ggcaagaagg ctcggggcac ggccgataag acgcccaggc aggacacgac gaccgcccgc
      901 cgggaagctc cggacagggc cggtaagggc gttacgacac cggcgggcag gcggtcgcgc
      961 ggcggttccg aaagccgcat acgcgatccc tattcgataa gcatggagga acggctacgc
     1021 ctgtaccgcg agcgctacgg ttcagccgga gagccgaagc ccgccggagg caagccttcg
     1081 accgcgcagc cttcccggtc cagccaggaa cgcaagcgat ccgaaccccg cacggccccc
     1141 gtccaacgag cgccagccgt ggagtccgta cccgtgcccc cgccggatcc catgcccagg
     1201 ggtatcctgg gcgccatcaa gggactcttc ggcaagaagg acgagtaggc aagcccgacg
     1261 ctacgctatc cgaccaagcc ttagatcgcc tggctggcct cgagggctcc cgttaccggg
     1321 agctcggctc ttcgttgcgc ttcgcctcag gcagccgccc ttgcgttcct gcttggaggg
     1381 gcgacgacga cgctgccggc gatgtcggcc gtattcggca taaccaaacc gcgcgtcttc
     1441 gccgtatacc tcggctcggc cgtagcggcg gcactactgt ccggcctcgc ctgggccgcg
     1501 ttcttctgat cgctgacgag agaattgaac cgtgcgtatg cgaagcgccc tggccaagta
     1561 cgaggaagcg atagtcggga tctacaatcc gtacgtaacg acgatcacct tcgaggagac
     1621 gccggtaggc gcgaacgaga tgggggaacg catagtcaag aacggcgggc ggctcctcgc
     1681 ccggttctag gtctgtagcc ccctggccag ctcacaggcc ccggtctggg cgtcgccctt
     1741 ggcgtcgacg acctcgacgt ccgggtggga ctcgcgcagg attgccgtga cggccgaccg
     1801 tagctcggcg ttcctctgga tgaagccgcc gtatggagcg aggcgccgac ggatcatgcc
     1861 cgggctgagt cgtgtgtaca ccgacgagac gagggccgcc agctcgaggg cggcctggtc
     1921 catgatggat cgcgccaacc tgtcgcccga cgcggcgaga accgccacgc gcggcgcgaa
     1981 cgaggcgatg cgcgccttgt cgaagtccca gtacgccatc gggatgaact cgcgagcatc
     2041 cgcgaacccg aagaacgcca acgcctcctc gatcagtcca gtaggctcgt cccgcccctc
     2101 gatggatctg agcccccgct tgatcgcctc gaaggccacc cagaaagccg agccctcgtc
     2161 gccgaggaaa tgcccaagcc cgccggccct cgcgtgcctt ccgctccggt cccggccgta
     2221 ggctatcgac ccggtaccgg ctatgagcag gaagccttcg acgtcgtcga gcgcgccgac
     2281 gagggccggc tcggcgtcgt tggccgcgcc gaagcgcgct ccggcgacgg ccgtcgcggc
     2341 gaatcccgcc ctggttgcgg ccgagacgaa ggccacgcgc atcgcgccgg acagcgtctc
     2401 gcggtcggcg tccaggtcga cgccggccga gccgatgaag cccgcccggc agcgcgccgg
     2461 gtcgagcccc gccgcgaagg cgcccgacac gagcgtctcg agcgtggccg tgaccgcctc
     2521 ggtcgggttg gagttgaggt tggtgctgcc gccggagccc tcgtacagga cggccccgtc
     2581 catcgactcg gcgcgcagcc gtgaactcgt cccgccgccg tcgataccga agacccagtc
     2641 gttcatggta ggtagagcgc ccccgcgctg atggcgcgcg ccgcgccggt cgcctccggc
     2701 tccgtgttcg gccgccccag gagccggagc agcccgaaca gcccgaaggc catggcttcc
     2761 ttgtagtcta cggacaggcc gacctcgtcg gcgtctcgaa cctcgcactc cggcaacgcc
     2821 gcgcgcagct ccgccatgat cacggggttc ctggcgccgc cgccggagac gacgacgagc
     2881 ctggggaggg ctgggagcag ctcgcggtag gccctggccg cggacgccgc ggcgaaggtc
     2941 tcggcggtac ggatgaggtc ggcgtacaga gcctcgcggt tctggccttc cgggacgccg
     3001 cccaacaacg gccgtaggct cgattcgtag aacgcggagc cgaattcctc acgcccggag
     3061 ctcttgggag gggccttggc caggaagggg tggtcgagcc agcgtgcgag caggtcgcgt
     3121 cgcaggcttc ccctggagcc gatcagcccg tcgcggtcgt agccgagacg gccgtcggtc
     3181 atctcggaca caagccgatc ggcgatcatg ttggccggtc cggtatcgaa ggccacgagc
     3241 ggcccgttct ccgggatcca gcagacattt ccgatcccgc cgtagttctg gaacgctatc
     3301 ggcttatcga agcgggcgga cgcgatcctg tcgaagaagg gcacgagcgg cgctccggta
     3361 ccgccgacgg ctatatcgcc ggacctgaag tcgcagacgg tcgcgatgcc ggtgcgcgcc
     3421 gcgatcatgg cgcccgagcc tatctgcagg ctggcgcgcc tcggcgagcc cggggaacca
     3481 gccacgtgac gtattgtctg accgtgcgac ccgatgacgg ccacgtccga cggcttcagc
     3541 cccgcctcgc gtatggccgc cagggcggcg ccggcgaacg cctcccccag ctccatgtcg
     3601 aggagcgcca acgcatccag agagcccgcg ccgtcctcga acagcgagaa gacggccgcg
     3661 cgcgccgaat ccgccagcgg gacgccgatg gccgcgacca ggcgcgatcc caccccgccg
     3721 tcgccatcga cgacgtgctc cgataccgcc gcgtcgatgc cgtcgacgct cgtcccggac
     3781 atcagcccga cagaatagta tcgatcggtc acgccccgcc tcccgtcgac cggtaggcat
     3841 ccaccgcctt ggacaggtgg ccgtcgttgg ccgccacgag ccgctccgcc tcgtcgactc
     3901 cgatgccgag gaggaccatc aggatggcta tcttgggctt ccggccggac tcggcgaagg
     3961 cccgctcggc ggtggctcgg tcgcattccg cggccctcat gatcaggttc ttggagcgtt
     4021 cgaccagctt cttgttgacg gggcgcaggt ctatcatcag gtttcggtag accttgccga
     4081 gccgtatcat ggcggtcgtg gtgatcatgt tcaatacgag cttctgggcg gttccggatt
     4141 tcatcctcgt cgatccggtg acgacttcgg ggccgacgtc caggaagatc ctgtggtcgg
     4201 ccagcgcgaa ggtgccggaa ttggcgttgc acgatatggc cccgacgacg gccccgagct
     4261 ccctggcccg gcgcatcgcc ccgagcacgt acggcgcctg gcccgacgcg gtgatcccga
     4321 cgagcacgtc gccgggcccg aaaccgacgc cgtcgaggtc cttgacaccc atctcctccc
     4381 tatcctcggc ggactcgatc gagcggacga gggcctcacg gccgccggcg atcagcccct
     4441 gcaccatgcc gggaggggta ccgtaggtcg gggggcactc ggaggcatcg agcacgccaa
     4501 ggcggcccga ggtgccggcc ccgatgtaga cgagcttgcc gccgcgtctg aaggactcga
     4561 cgacgtcgtc gaccagggcg gctatattgt cgagcgccgg ctccaccgcg aagggcacct
     4621 tcttatcctc ggcgttgatg atagccagta tctcccgcgt acccttcgag tctatgccga
     4681 ggctggcggg attctcgcgc tcggtcatca agccggagag gtcgatcatc tctctagccg
     4741 tatcctcagg ctcattcgtt cgtaacgttt ccataccttg aatcctagct ttccgtaaaa
     4801 atccaccaag tcggtccaat cgatgaccat cgtgccgacg ccccttcgcg ccaggtcgcg
     4861 gacgcagtgc tccagcaggc ccatcccaag cccgagccct cgcctggatg gatcgacgcc
     4921 gatggggccg agcccgccgg ggtgctcccc catcagagcc cgccaataca cccccggccc
     4981 gagcaggggg ctgtccttat cgtatatacg cgagaagccg accaccgatc cgtcgcgctc
     5041 atccacggcc aacatcaggt cggcgccgcg gatacccgcg tcgagggctt cccggatctc
     5101 gtgcgaccag cgccccggga agctcgagcc gatgaagcgc aggagggcgc cacggtactt
     5161 gggctcgtag agcgcatagc ggtacgaacc gttcggcaac ctctcgggct cgctcagcca
     5221 ggcggcgcgg gtcagatcgg ccgacaggtc gtattcgaag ccctcgccct cgaagccgac
     5281 ggcccggaga aaggcgtcga gagcctcgaa gccggcccca tcctcgacgg gccgcccagg
     5341 tacgaggtga taatagtcgc cgccgagctc gagtcgctcg gccccccgcg acgcaagcca
     5401 ggcgaccgac cgggccagga gcccggcggc gagaccccgc ctccgttcgg cctcgtccac
     5461 cgccaggagg gacaggtagc cgatcctggg cgctacgcca tccgcccccg gccggacggc
     5521 ccgcttgacc agcgaggccg ctaccaggga tccgtcgggc ctggcgacag ccaggcatag
     5581 ccggggatcg gtatcgagcg ctacttgctg gcgtagcagc cgctcatcga gcgggaaggc
     5641 ggtcccggcc gagcggttcc acagggccgc cagctcggcg gcgacggctt ctgggtctac
     5701 ggcccgtatc gtcatgctaa cgtggctcca cggctttcag gtcctgctca tgcacggcct
     5761 tcaggctacg ctcgagcctc gggatcgaca cctcgatgct gcggcatatg atggtggcgt
     5821 acaggatgtc caccatgacc agctgggtgg ccctcgacag cgaggcgccc gtacggagca
     5881 gcggctcggt ggccggcacg tagagcgcgg tgtcggcgag cctcgacaga gggctctgac
     5941 cgacgctggt aatgcctatc aacctagcgc cggagcgctt ggcctcctcg acgatggtga
     6001 ccgtgtgggc cgatctgccg gagtacgaca cggcgactgc ggcgtcggac ccgccgaggc
     6061 cgcaggctat gctgacctgg acgtcggcgt cgaacgcgaa ggagcagggg acgcctatgc
     6121 gcgatagctt ctggtagagg tcgtaggcca cgttggccga ggcgcccacg ccgaagatag
     6181 cgacgcgcct ggcaccgagt atcgcggtgg ccgcctcctc gaccgcggag gtattgagca
     6241 ggctgagcac ccgctccacc gcgtcggtcg tcctggagac gatggtcctg gctatcgcgt
     6301 ccaccgactg gcttgaatcg agctcgaacg ccgggggcgg ctcttcgtca gcctggtcgg
     6361 tcgtcgcgta caggtccctg gtcagcaata tctgtagctc ccggtaaccg gatagcccgg
     6421 cccgcttgca gaggcgtatc acggccgggg ggttggtatc cgcctgcctg gccagttcgg
     6481 cgatggtgca gaacaccgcc gttttaggat tttctatcaa gtactcggcg atcttgcgtt
     6541 cagattcggt acatgaatcg acgatctcgc gtagaagcgc cactccgctc gtcatcgttg
     6601 cattgtcctc gccatcgggt tagactcatc acgaatgagc cgtagtatac catgcttttc
     6661 tataagcgtg gaatatcatt tctttaaaac aaggcttttc ataaaatatt gttgcgatcc
     6721 tcttgggaat ggaagatact agcatcatga agaccatcgc gaatatgacg cttgaacaga
     6781 aaatcggaca gatgctcctg ataggctacc cgggaggagc cgccggactc cggacgctgg
     6841 aacgcgtcgt gtcgcgacgg ccaatgggca acatcatact cttctcgcgc aacgcgaagg
     6901 ctcccgggcc gctccgcgat gagctggcct acgtccgctc gatgatagcc ggccaggccg
     6961 gcgtcgagcc gctcgtctcg atagaccagg aaggcggcat cgtcaccagg ctgggggccg
     7021 gcttcacgcc cctgcccggc gctatggcgc tggccgccgc cgtctccggc ggcgcggcga
     7081 cgacggccga catcgaggcg atggccacgg tcaacggctc
//
LOCUS       DAOPHV010000060         6961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_13944_length_6961_cov_4.727773, whole genome shotgun sequence.
ACCESSION   DAOPHV010000060 DAOPHV010000000
VERSION     DAOPHV010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13944_length_6961_cov_4.727773"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PLQ29_02690"
     CDS             complement(<1..737)
                     /locus_tag="PLQ29_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85582.1"
                     /translation="MEKYDICDGVSVVGIPEAGLSVLCGCPENVVKFMIRGGVIRSEM
                     VGGRRFETGPNAILLSELPIQNGRFSNLAEFPVLQMLYRQGLIVPGHPRNTGVRPMLV
                     GLRAQVEAQARYIYAGTYGITEPEALDPDDPTAAAELIRMKRKFSFGAFRSVDELLDV
                     RVIDGPVVGLRGGAFLRRLGVNRYEFICGGSAVDVDLNLGPGASYGAPYELPRSRISH
                     EAFAVVHLGEGDGWDPERPCMCSLVVHA"
     gene            902..1747
                     /locus_tag="PLQ29_02695"
     CDS             902..1747
                     /locus_tag="PLQ29_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPG85583.1"
                     /translation="MRKFSIVVMALAALSVTSAFALGGKEPAAGSGPAAAAAIGASGL
                     PDLGGRVIVAVTENAYTPLNFVDPKTGKAMGWEYDAVNEIARRLNAKVDWQLSSWDLM
                     IQSVRDGQFDVGMDGITITDERKKQVDFSDPYMVSQQFMLVRADEKRFSDAAAFAADP
                     KLLVGAQAGTTNFYVAVYEVLDGDEQNKRIKLFETFGATVQALIAGDVDMVLMDAASS
                     KGYVGANPGKLKSVGGPLGTEEFGFIFPPGSDLVGPFNAALASMKADGTLDRLNTKWF
                     FEYQP"
     gene            1776..2594
                     /locus_tag="PLQ29_02700"
     CDS             1776..2594
                     /locus_tag="PLQ29_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPG85584.1"
                     /translation="MTNGRRAARIAAGVPWWLVLLVGLGAIALGTALADASYRVIFGA
                     VARGFGVTIGVALASYAASLALGLLLGLARSSRLRPLREASTFYIELVRGLPMLVILY
                     YIAFVGAPALAEVLNGLLAPLIRAGAVKAFQARDFSFATRAGLALTLGYAAFIAEIFR
                     AGIESVDKGQIEAAHALGLSHSQTMLRVVLPQAVKNVLPALANEFVAIIKDSALVSVL
                     GVQDITQIGKVYAASTFKFFETYNVVAFFYLCMTVSLSMLVRALERRLKRENAR"
     gene            2591..3337
                     /locus_tag="PLQ29_02705"
     CDS             2591..3337
                     /locus_tag="PLQ29_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HPG85585.1"
                     /translation="MNAFIRVVDAVKTFGDPERGGVRALDGVSLEVAKGEVVLVIGPS
                     GSGKSTLLRAVNRLERLDSGGIWIGDDEVTARRADLRRIREDAGMVFQSFNLFSHMSV
                     LDNVTLAPRLVRKASAAAAREAGRAILERVGLGDKLEAYPRQLSGGQQQRAAIARALA
                     MSPKALLFDEPTSALDPEMIKEVLDVMTALADDGMTMMVVSHEMGFARAAADRVVFMD
                     RGRIVEQGTPDELFASPREERTKEFLGHIL"
     gene            3373..4230
                     /locus_tag="PLQ29_02710"
     CDS             3373..4230
                     /locus_tag="PLQ29_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HPG85586.1"
                     /translation="MSRSLVYQTDFGISDGAVAAMYGVALKVDPALAIFDLTHDIPQY
                     NIWEASYRLVQTVQYWPAGTVFVSVVDPGVGSSRRSVAVKTAGGQIVVTPDNGTLTHL
                     KLLQGVTDARLLVEDANRLSGSELSYTFHGRDVYAYTGARLASGQIAFEDVGPSVEPA
                     SIVELDHVDAVRSGDAIEGTIDVLDVRFGSLWTNIPHSLFIGLGVDYGKRVEVAIRND
                     TREVYRNTMVYARSFAEVSLGEPLVYINSVLNVAAAINQGSFARAYNIGTGLSWKIAI
                     RRAPRIVYD"
     gene            complement(4227..6647)
                     /locus_tag="PLQ29_02715"
     CDS             complement(4227..6647)
                     /locus_tag="PLQ29_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HPG85587.1"
                     /translation="MNRKHSRAPWRFFTVAALFVVATGAFAQSGGSADPHTVWDYADN
                     PAAWSLFPDAIAFGVRAEAAVSDLASFAGGTLPATVDLAYGLPGLVYRYRDDGSSSTH
                     ALTSAFSFGRSFGLGLRFRWDGTTTSAWCVQDLGVMLRPFGYASVSASVDDVLDLSGD
                     GYEAALGLAVRPLAFNPRLGSALTLSADARLGPDGFAMAGAGARFVLADWLSLSGSYS
                     FDDASFGLGFAVSLSGAETSVGGRLNGMHQGGDVSLGQSVRLGRAAKSAERVFGSSVL
                     VIDEPGLFQPNPPRVDMDPGFGDEVALWFDLAVAAIDRAASDASIRALAMFEPPLFDT
                     DARAQEFCRALGRFRVAGKPVYVFAQSMGRLSYVYAAAGAELVALDPNGGLPIVDVSS
                     THLYFKDLLARLGVRTYSLQSHDTKTANNAMTEPSMTGPERAMIERYVGGLASQAWAS
                     LDEARGDRVVGGGRAAIGAGPYLDPRKAVEAGLADELLYRDEFEERLTELTAGAGRVD
                     LRSYARERGLEWGPPPGRKVAVVYLSGSIIEGEGVAGSSIGESAAELLAALRDDPTVA
                     GVIMRVDSGGGSALTSDHIARQVKKLKDSGKPVVVSMAGYAASGGYYISAYADRIIAE
                     AGTLTGSIGVTGFDINLTGTLELLGVGSGVVSAGPSGEFGNPFLPRREADGQAERGMI
                     MYIYERFVNVVAEGRGMDRARVDELGKGQIWLGSEAVENGLVDALGGLVDAKAAMEEL
                     LGAAGRFEVYLPGTLEPGSILSLLGLAKAGGASSSMLASARAFVNELAELGEGALYLA
                     PEYLYRER"
     gene            6751..>6961
                     /locus_tag="PLQ29_02720"
     CDS             6751..>6961
                     /locus_tag="PLQ29_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85588.1"
                     /translation="MPVEHRKPFSAASALRRAAAYAASFPKPIWLLVAATLVESTGRF
                     MVVPYLSLYMKGEGVSLGALGFVLGA"
BASE COUNT         1166 a         2374 c         2369 g         1052 t
ORIGIN      
        1 gcgtggacga cgaggctgca catgcacgga cgctccgggt cccagccgtc gccctcgccg
       61 agatgcacca cggcgaacgc ttcgtggctt atccgcgatc ggggcagctc gtacggcgct
      121 ccgtacgacg ctcccggccc gaggttcagg tcgacgtcga ccgccgaacc gccgcagatg
      181 aattcgtagc gattaacgcc gagccgcctc aggaaggcgc cgcccctcaa ccccacgacg
      241 gggccgtcga tgacgcggac gtcgagcagc tcgtcgacag aacggaacgc gccgaacgag
      301 aatttgcgct tcatgcgtat cagctcggcc gccgccgtcg ggtcgtcggg atcgagcgcc
      361 tcgggctcgg tgatgccgta ggtgccggcg tagatgtagc gggcctgagc ctcgacctgg
      421 gcccggagcc cgacgagcat cgggcggacg ccggtgttgc gcgggtggcc gggcacgatc
      481 aggccctggc ggtacagcat ctggagcacg gggaattcgg cgaggttcga gaaacggccg
      541 ttctggatcg gcagctcgga taataggatg gcgttcgggc cggtctcgaa acgcctgccg
      601 cccaccatct cggaacggat gacgccaccg cgtatcatga acttcacgac gttctcgggg
      661 caaccgcaga gtacggacag cccggcctcc gggatcccga cgacggagac gccgtcgcag
      721 atatcgtact tttccatacg aaagcccccg atccatggac gcggcggctg ggccggacgc
      781 caggccgccg ccacctcgcc tctagtataa tgcattccag tccggcgggc gcccttgcct
      841 cgagccgaga tcggtgcaac aatggtcgca ggcggccccg cgccgttcag gaggtaccga
      901 tatgaggaaa ttcagtatcg tcgtcatggc gctcgcggcg ctgtccgtaa cgtccgcctt
      961 cgccctcggc ggcaaggagc ccgccgccgg atccggcccc gccgcggccg ccgccatcgg
     1021 cgcgtctggc ctgccggacc tcggcgggcg cgtcatcgtc gcggtcaccg agaacgccta
     1081 cacgccgctc aacttcgtcg accccaagac cggcaaggcg atgggctggg agtacgacgc
     1141 cgtcaacgag attgccaggc gtctcaacgc caaggtcgac tggcagctat cgagctggga
     1201 cctgatgatc cagtcggtcc gtgacggcca gttcgacgtg ggcatggacg gtatcaccat
     1261 caccgacgag cgtaagaagc aggtagattt ctccgacccg tacatggtct cgcagcagtt
     1321 catgctcgtg cgcgccgacg agaagcgctt ctccgacgcc gcggccttcg ccgccgaccc
     1381 caagctgctg gtcggcgccc aggccggcac caccaacttc tacgtagccg tctacgaggt
     1441 gctcgacggc gacgagcaga acaagcgcat caagctgttc gagaccttcg gcgccaccgt
     1501 gcaggcgctg attgccggcg acgtcgacat ggtgctgatg gacgcggcct ccagcaaggg
     1561 ctacgtcggg gccaacccag gcaagctcaa gagcgtcggc ggcccgctcg ggaccgagga
     1621 attcggcttc atcttcccgc cgggatctga cctggtcggc ccgttcaacg ccgccttagc
     1681 ctcgatgaag gccgacggca cgctcgatcg gctgaacacc aagtggttct tcgagtacca
     1741 gccataatac cgggcggagc gtagggggac ggaggatgac gaacgggagg agagccgcca
     1801 ggatagcggc gggcgtgccg tggtggctcg tcctgctcgt cggcctcggc gccatagcgc
     1861 tcggcacggc gctggcggac gcgtcgtacc gggtcatatt cggcgccgtg gccaggggct
     1921 tcggcgtgac gataggcgtg gcgctcgcgt cgtacgcggc gtcgctggcg ctcgggctcc
     1981 tgctcgggct ggcccgatcg tcgcgcctgc gccccctgcg cgaggcgtcg acgttctata
     2041 tcgagctggt gcgcggccta ccgatgctcg tcatcctcta ctacattgcc ttcgtcgggg
     2101 ccccggccct ggccgaggtc ctgaacgggc tgctggcgcc tctcatccga gccggcgccg
     2161 tcaaggcctt ccaggcccgc gacttcagct tcgcgacgag agccgggctc gcgctgaccc
     2221 tcggctacgc ggccttcatc gctgagatct tccgcgccgg catcgagagc gtcgacaagg
     2281 gccagataga ggcggcccac gcgctcgggc tgtcgcactc gcagacgatg ctgcgcgtcg
     2341 tgctgccgca ggcggtcaag aacgtgctgc cggcgctggc caacgagttc gtcgctatca
     2401 tcaaggactc ggccctggtg tcggtactcg gcgtgcagga catcacccag atcggcaagg
     2461 tctacgccgc gagcaccttc aagtttttcg agacctacaa cgtcgtggcc ttcttctacc
     2521 tctgcatgac cgtgagcctg tccatgctgg tacgcgccct cgagcgccgg ctcaagcggg
     2581 agaacgcccg atgaacgcct tcatacgagt cgtagacgcg gtcaagacct tcggcgatcc
     2641 cgagcgcggc ggcgtgcgcg ctctcgatgg cgtgagcctc gaggtggcca agggagaggt
     2701 ggtgctcgtg atcggcccct ctggatcggg taagagcacg ctgctacgcg cggtcaaccg
     2761 gctcgagcgg ctcgactcag gcggtatctg gataggcgac gacgaggtga cggcgcggcg
     2821 ggccgatctc aggcgcatcc gcgaggacgc cggcatggtc ttccagagct tcaacctgtt
     2881 ctcgcacatg agcgtgctcg acaacgtgac gctggcgccg cgcctcgtgc gcaaggcgtc
     2941 ggccgccgcc gcacgggagg ctggccgggc catactcgag cgcgtcggcc tcggcgacaa
     3001 gctcgaggcc tacccgcgcc agctgtccgg cggccagcag cagcgcgccg cgatagccag
     3061 ggcgctcgcg atgagtccca aggcattgct gttcgacgag ccgacctcgg ccctggaccc
     3121 ggagatgatc aaggaagtgc tcgacgtgat gaccgcgctg gccgacgacg gcatgacgat
     3181 gatggtcgtt tcgcacgaga tgggcttcgc gagggccgcc gccgaccggg tcgtattcat
     3241 ggaccgcggc aggatagtgg aacagggaac gcccgacgag ctgttcgcca gcccgcgcga
     3301 ggaacgcacc aaggaattcc tcgggcacat cctgtaggac aacggcgccg cgacaggcgg
     3361 tatggaggcg cgatgagccg ttcgctcgtg taccagacag atttcggtat ctcggacggc
     3421 gccgtcgcgg ccatgtacgg agtggcgctc aaggtcgacc cggccctggc catcttcgac
     3481 ctgacgcacg acataccgca gtacaatatc tgggaggcct cgtatcggct cgtacagacg
     3541 gtccaatact ggccggccgg caccgtgttc gtgtcggtcg tcgacccggg cgtcggcagc
     3601 tcgcgacgta gcgtcgcggt caagacagcc ggcgggcaga tcgtcgtgac gccggacaac
     3661 ggtacgctga cccacctcaa gctgctgcag ggcgtgacgg acgctcggct cctggtcgag
     3721 gacgccaacc gcctgtccgg ctccgagctg tcctatacct tccacggccg ggacgtgtac
     3781 gcctacacgg gcgccaggct cgcctccggg cagatagcct tcgaggacgt cggcccgtcc
     3841 gtcgaaccgg cctccatcgt cgagctcgac cacgtggacg cggtccgctc cggtgacgcg
     3901 atcgagggca cgatagacgt gctcgacgtg cgtttcggta gcctatggac caacattccg
     3961 cattcgctat tcatagggct cggcgtcgac tacggcaagc gcgtcgaggt cgcgatccgc
     4021 aacgacacgc gcgaggtcta ccgcaacacg atggtctacg cgcgctcctt cgccgaggtg
     4081 tcgctcggcg agcccctcgt ctacatcaac tcggtgctga acgtcgcggc ggcgatcaac
     4141 cagggctcgt tcgcccgggc ctacaacata ggcaccgggc tgtcctggaa gatcgccata
     4201 cgcagggcgc cgaggatagt ctacgactag cgctcccggt acaggtactc gggagccaag
     4261 tacaaggcgc cctcgcccag ctccgccagc tcgttgacaa aggctcgagc gctagcgagc
     4321 atcgacgacg acgcgccgcc cgccttggcc aagccgagca gcgacaggat cgagcctggc
     4381 tcgagcgtac cgggcagata gacctcgaaa cggcccgccg cgccgagcag ttcctccatc
     4441 gcggccttgg cgtcgacgag ccctcccaac gcgtcgacga gcccgttctc cacggcctcc
     4501 gagccgagcc agatctggcc cttgccgagt tcatcgaccc gggccctgtc catgccgcgg
     4561 ccctcggcca ccacattcac gaagcgctca tagatataca tgatcatgcc gcgctcggct
     4621 tggccgtcgg cctcgcggcg cggtaggaag gggttgccga actcgccgct cgggccggcg
     4681 ctgacgacgc cggagccgac gccgagcaac tcgagcgtgc cggtcaggtt gatatcgaag
     4741 ccggtaacgc ctatcgagcc ggtcagggta cccgcctcgg cgatgatcct gtcagcgtag
     4801 gcggagatgt agtagccccc ggacgcggcg tagccggcca tcgacacgac gacgggcttg
     4861 cctgagtcct tgagcttctt gacctgcctg gcgatgtggt cgctcgtcag cgcggagccg
     4921 ccgcccgagt cgacgcgcat gatcacgccg gccaccgtcg ggtcgtcgcg cagcgcggcg
     4981 agtagctcgg cggcggactc gcctatcgac gagccggcta cgccctcgcc ctcgatgatg
     5041 gagccggaca ggtagaccac ggccaccttc ctgccgggcg gagggcccca ctcgaggccg
     5101 cgctcgcgcg cgtaggaacg caggtcgacg cggcccgcgc cggcggtcag ctcggtaagc
     5161 cgctcctcga actcgtcgcg gtacagtagc tcgtcggcga gcccggcctc gacggccttg
     5221 cgcggatcga ggtacggccc ggcgccgata gcggccctgc cgccgcccac gacgcggtcg
     5281 ccgcgcgcct cgtcgagaga ggcccaggcc tgcgacgcca ggccgccgac gtagcgctcg
     5341 atcatagcgc gctcgggtcc cgtcatggac ggctcggtca tcgcgttgtt ggccgtcttc
     5401 gtatcgtggg actgcaggct gtaggtcctg acgcctagcc tggcgagcag atccttgaag
     5461 tataggtggg tcgacgagac gtcgacgatg ggcaggccgc cgttcgggtc cagggcgacg
     5521 agctcggcgc cggccgcggc atagacgtat gacagccggc ccatcgactg ggcgaacacg
     5581 tagaccggct tgccggctac ccggaagcgc ccgagcgcgc ggcagaattc ctgggcgcgc
     5641 gcgtcggtat cgaacagggg cggctcgaac atggccaggg cccggatcga cgcgtcggag
     5701 gcggcgcggt cgatggcggc gaccgcaagg tcgaaccaga gggccacctc gtccccgaag
     5761 ccggggtcca tgtcgacgcg gggcggattc ggctggaaca gtcccggctc gtcgatgacg
     5821 aggactgacg acccgaagac gcgctcggcc gacttggcgg cccggccgag ccgcacggac
     5881 tggcccaggc tgacgtcgcc gccctgatgc atgccgttca gccgcccgcc gaccgaggtc
     5941 tcggcgccgg acagcgagac ggcgaagccg aggccgaagg acgcgtcgtc gaacgagtac
     6001 gagcccgaga gactgagcca gtcggccagg acgaacctgg cgccggcgcc ggccatagcg
     6061 aagccgtcgg ggccgagccg ggcgtcggcg ctcagcgtga gcgcggagcc gagcctcgga
     6121 ttgaaggcga gcggccggac tgccaggccg agcgcggctt cgtagccgtc gccggacagg
     6181 tcgagcacgt cgtcgaccga ggcggagacg gacgcatagc cgaagggcct gagcatcacg
     6241 ccgaggtcct ggacgcacca ggccgacgtg gtcgtcccgt cccaacggaa gcgcaggccc
     6301 aggccgaagg agcggccgaa gctgaaagcg ctcgtcaggg cgtgggtcga cgagctaccg
     6361 tcgtcgcggt agcgatagac gagtccgggc aggccgtagg ccaggtcgac agtcgccggc
     6421 aaggtaccgc ccgcgaacga ggccaaatcg gatacggcgg cctctgcccg cacgccgaag
     6481 gcgatcgcgt cggggaagag cgaccaggcg gccgggttat cggcatagtc ccagaccgta
     6541 tgcgggtcgg cggatccgcc cgactgggcg aacgcgccgg tcgcgacgac gaacagcgcc
     6601 gcgacggtaa aaaagcgcca tggcgcccga ctatgctttc ggttcatgaa cggtctcctc
     6661 gaggacagta tatcgtacca aaactcgcta ctaccatatc gcgccagacc atagccgacc
     6721 acgtttcgtt gtcgctatac tcgccgcacc atgcccgtcg aacaccgcaa gcctttctcc
     6781 gccgcgagcg cgctgcgccg cgccgccgcc tacgccgcaa gcttcccgaa gccgatctgg
     6841 ctcctcgtcg ccgccactct ggtcgagtcc acggggcgct tcatggtggt gccgtacctg
     6901 agcctgtaca tgaagggcga gggcgtcagc ctcggcgctc tcgggttcgt gctcggcgcg
     6961 g
//
LOCUS       DAOPHV010000061         6850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_14230_length_6850_cov_3.833701, whole genome shotgun sequence.
ACCESSION   DAOPHV010000061 DAOPHV010000000
VERSION     DAOPHV010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14230_length_6850_cov_3.833701"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /gene="ccsA"
                     /locus_tag="PLQ29_02725"
     CDS             <1..770
                     /gene="ccsA"
                     /locus_tag="PLQ29_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPG85589.1"
                     /translation="FAALLASAALQVFFLFKRPAKPDKVAPALMLASAAALLAVIAMR
                     SVRIGFIALTSTYESLAFWAAVVCVVSFVYGRQRRLQYRPGVVFGATMLSVVMLAVAS
                     SPLIPKEALPPIPALRSAWLILHVSFTFVGEAFFVVSFVTALIFLAVKDDDRRLELDR
                     LTYTAIAVGYPFFTAGALVFGAIWAEQAWGRWWSWDPKETWALVTWLVYTAYLHVRLV
                     MRRKDAVPSVLAVVGFICTAFTFFGVNYLLPGLHSYG"
     gene            complement(761..2116)
                     /locus_tag="PLQ29_02730"
     CDS             complement(761..2116)
                     /locus_tag="PLQ29_02730"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HPG85590.1"
                     /translation="MRRIVLWALVFAGAALALAFGISALLRTSHEAEALAQEASHAAL
                     GIEAYSSSVSSLDDPRVVSVIILFAILSASAAFSSSVSALMLRRRVSSQHLELRRKVH
                     ELEALDARHNAMLSALPDLVFVFDAEGRIIDYLKSAINTFAVPPERFIGKTIAEVFGD
                     AELDGRIREAIHRTLSGHGPQILEYTLGGIEPNERWEARLVKATDSSILSVNRDVTER
                     FNAERERESSLLEKEALLKEIHHRVKNNLQIVSSLLSMQSAKARDEYDRRLFMDSQSQ
                     IRAMAAVHDQLYRSSDFSSIAGGAYLTDLVSGLEASWARPGIWVRASFEPGDEGLELD
                     LERAVPIGLIVNELITNAYKYAFEGRHTGSIRASLVKRDDGGLILSVSDDGKGLPDGF
                     DASVGDGGMGFTIINALVMQLGGRLEAGSPEGDTPGSGGTVVTVTVPPRGEPPRKRDQ
                     P"
     gene            complement(2113..3207)
                     /locus_tag="PLQ29_02735"
     CDS             complement(2113..3207)
                     /locus_tag="PLQ29_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPG85591.1"
                     /translation="MDIRIKPKPASALESTPLVQQTTLWARVKRMLGWSAMAFDLDAS
                     GEPAGDVLVVVRDVGGGSSVAYVPYGPEGLPDDGVRGEYLAALSVELRAHLPAECIMT
                     RWDLPWESPYALEEDRYDDEGHWLGAPETRVREARMNFGVRGGALRKAPSDILPPDTL
                     IVDLRREPDELLAAMRPKTRYNIGLAARRGVTVREGGADDLPLWRALYEDTARRNRMS
                     PQPDDYFSAVLRASRGSGPSAGDESITMLVAEKAGRPLAAMFMSVSADRAAYLYGASS
                     SDDRNLMAPYALQWAAMNRARSAGCLSYDLFGVAPRPDPDHSMYGLYVFKSGFGGSML
                     HRQGAWDYAYDEVGYASYVAYEAAGPRFIA"
     gene            3344..4510
                     /locus_tag="PLQ29_02740"
     CDS             3344..4510
                     /locus_tag="PLQ29_02740"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPG85592.1"
                     /translation="MDSSDQPRDGRFPRLAVDLGIIENNARFVRQACARSGIGLVAVV
                     KSVGARIPIVAAMARGGCRSFASSRLEQLALLREAALDPGSGVPPDATTGLLRATGRS
                     EAAAAVALADWSLQSEAAALEATADAAEARGVRHGVTLMLDLGDLREGWFDRQELVAA
                     ALRVERGLPSLRLRGIGTNLGCYGAVLPTRENLGRLLEAAREVEDAIGRRLDVVSGGA
                     TTSLPLVARGRMPAGVNELRIGEAILCGKDLPLFHRVELPGVRGDTITLRAELLEKHV
                     KPSYPIGARLRDAFGACPEYEDRGTRTRLLLGVGKRDIGNHELLRPRDGRIHIVGSSS
                     DYLICEADEPCDDLRYGSILEFDMLYGAMLFLCDANPYLSVDYRAAEGSRPVEP"
     gene            complement(4512..4994)
                     /locus_tag="PLQ29_02745"
     CDS             complement(4512..4994)
                     /locus_tag="PLQ29_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HPG85593.1"
                     /translation="MSEPCPCGSGRDYAECCGPIIAGKAKAPTAEALMRSRYTAYAKG
                     AVDHILSSCVQDDGIDAEATRDWSESADWKGLTIVRTEKGGPGDDEGVVEFVARYEMD
                     GLKEEHHETARFVREGGAWLYDSGEVKTATVVRATPKVGRNEKCPYGSGKKYKQCCGK
                     "
     gene            5131..6048
                     /gene="glsA"
                     /locus_tag="PLQ29_02750"
     CDS             5131..6048
                     /gene="glsA"
                     /locus_tag="PLQ29_02750"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HPG85594.1"
                     /translation="MQDALMRARLASRSACSGGTVASYIPALAGADGSALGLAAVDAD
                     GHVYEAGDSRSRFTIQSISKVLALAYVMTELGEGAVFSRVGKEPTGDPFNSIIRLETS
                     AGRKPFNPLINAGAIVVSSLMPGDGPANKGERFRSFVGGLVGRTSVERDDAVYQSEKS
                     TAARNRSIAWFLKELGLLEGEVEDALDVYFRQCAVAMDAVELARVGAILALDGVDPVS
                     GSRALAARTARVCKSLMVTCGMYDGSGEFAVDVGVPAKSGVGGGIMAAVRDRLGLGVY
                     GPALDERGNSAGGLAALRLLSDELDLRVL"
     gene            complement(6238..>6850)
                     /locus_tag="PLQ29_02755"
     CDS             complement(6238..>6850)
                     /locus_tag="PLQ29_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPG85595.1"
                     /translation="DFHPTQILADLLTIEEHVKKPLNKVKLVYCGDARNNMGNSLMIG
                     SAKMGMHFVALAPKELWPNEKLTAEMRDVAKSTGAVIEFFDISKADEALKGADALYTD
                     VWVSMGEEAKFAERIKQLAAYRVTMDFIRKTGNPDCIFLHCLPAFHDQETEVGRQVGK
                     EHGVMEQEVTDEVFRSRHSVVFDEAENRLHTIKAVMVATIGRQ"
BASE COUNT         1029 a         2313 c         2329 g         1179 t
ORIGIN      
        1 cgttcgcggc attgctcgcc tcggcggcgc tgcaggtgtt cttcctgttc aagcggccgg
       61 ccaagccgga caaggtcgcg ccggcgctga tgctcgcgtc cgccgcggcg ctgcttgccg
      121 tgatcgcgat gcgcagcgtc cgtatcggct tcatcgcgct gacgagcacc tacgagtcgc
      181 tggcgttctg ggcagccgtc gtatgcgtcg tctcgttcgt ctacggccgc cagcggcgcc
      241 tgcaataccg gccgggcgtc gtcttcggcg ctacgatgct gtccgtcgtc atgctcgccg
      301 tcgccagctc gccgctgatc cccaaggaag cgctaccgcc tatcccggcg ctccgctccg
      361 cttggctcat cctgcacgtc agcttcacct tcgtgggcga ggccttcttc gtcgtcagct
      421 tcgtgacggc gctgatcttc ctggcggtca aggacgacga ccgccggctc gagctcgacc
      481 gcctgaccta taccgcgata gccgtcggct acccgttctt caccgccggg gcattggtat
      541 tcggggctat ctgggccgag caggcctggg gccgctggtg gagctgggat cccaaagaga
      601 cctgggccct cgtcacctgg ctcgtctata ccgcgtacct gcacgtgcgc ctcgtgatga
      661 ggcgcaagga cgccgtgccg tcggtgctag cggtcgtcgg cttcatctgt acggccttta
      721 ccttcttcgg ggtcaattac ctgctgcccg gcctgcacag ctacggctga tcccgtttcc
      781 tgggcggttc gccgcgaggc ggtacggtaa ccgtgacgac ggtgccgccg cttccaggcg
      841 tatcgccttc cggcgagccg gcctcgagtc ggccgccgag ctgcatgacg agcgcgttga
      901 tgatggtgaa gcccatgccg ccgtcgccga ccgatgcgtc gaagccgtcg ggtaggccct
      961 tgccgtcgtc gctgaccgat agtatgaggc caccgtcgtc gcgtttcacc aggctcgccc
     1021 gtatcgagcc tgtatgccga ccttcgaagg cgtacttata ggcgttcgta atcagctcgt
     1081 tgacgatgag cccgatcggc accgcccgtt ccaggtcgag ctcaagcccc tcgtcgcccg
     1141 gctcgaagga agcgcgcacc cagatacccg gtcgggccca gctcgcctcg agccccgaga
     1201 ccaggtcggt caggtaggcg ccgccagcta tcgacgagaa gtcgctcgag cggtagagct
     1261 ggtcgtggac agccgccatc gcgcgaatct ggctctggct gtccatgaac agccgccggt
     1321 cgtactcgtc gcgcgccttg gcggactgca tcgacaggag actcgagacg atctgcaggt
     1381 tattcttgac gcgatggtga atctccttga gcagggcttc cttctcgagg agggacgact
     1441 cgcgctccct ctcagcgttg aagcgttcgg tcacgtctcg gttgacgctc aggatggagg
     1501 agtcggtcgc cttgaccagc cgagcctccc agcgctcgtt cggctcgatc ccgccgagcg
     1561 tatactcgag tatctggggg ccgtgcccgc tcagggtccg atggatggcc tcgcgtattc
     1621 tcccgtcgag ctccgcgtcg ccgaagacct cggctatagt cttcccgatg aagcgttcgg
     1681 gcggcaccgc gaaggtattg atggcgctct tgaggtaatc gatgatacgc ccctcggcgt
     1741 cgaagacgaa gaccaggtcg ggaagggcgc tcagcatcgc gttgtgccta gcatcgaggg
     1801 cctcgagctc atgcaccttc cgccggagct cgaggtgctg cgacgagacg cggcgccgta
     1861 gcatgagggc cgataccgac gaactgaagg cggcgctcgc cgacaggatg gcgaacagaa
     1921 tgatcacgct gacgacccgc gggtcgtcga ggctggagac gctcgacgaa taggcctcga
     1981 tgcccaaggc ggcatggctg gcctcttgcg cgagcgcctc ggcctcgtgg ctcgtcctca
     2041 ggagcgcgct aatcccgaac gcgagcgcca gggccgcccc cgcgaagacg agggcccaga
     2101 gcactatcct cctcacgcga tgaaccgcgg cccggcggcc tcgtaggcta cgtaggacgc
     2161 gtaacccacc tcgtcgtagg cgtagtccca ggcgccctgc cgatgcagca tcgagccgcc
     2221 gaagcccgac ttgaagacgt acagcccgta catggagtgg tcgggatccg gcctcggggc
     2281 tacgccgaac aggtcgtagc tcaggcagcc cgctgacctg gcgcggttca tcgcggccca
     2341 ttggagcgcg tagggcgcca tcaggttgcg gtcatcgctc gacgaggcgc cgtagagata
     2401 agcggcgcgg tccgccgata ccgacatgaa catcgcggcg agcggccggc cggccttctc
     2461 ggcgacgagc atcgtgatcg actcgtcccc ggcgctgggg cccgatccgc gtgaggcgcg
     2521 caatacggcc gagaagtaat catccggctg cggcgacatg cggttcctgc gcgccgtgtc
     2581 ctcgtagagc gcccgccaga ggggcaggtc gtcggcgccg ccttcccgga ccgtgacgcc
     2641 acgcctagcg gccaacccga tattgtacct ggtcttgggc ctcatcgcgg cgagcagctc
     2701 gtcaggctcg cgcctgaggt cgacgatcag cgtatccggt ggcaggatgt cgctcggcgc
     2761 cttgcgcagc gcgccgccgc gcacgccgaa attcatgcgc gcctccctga cccgcgtctc
     2821 gggcgcgccg agccagtggc cctcgtcgtc gtagcgatcc tcctcgaggg cgtagggcga
     2881 ctcccagggc aggtcccagc gcgtcatgat gcactccgcc ggaaggtggg cgcggagctc
     2941 cacggacaga gcggccaggt attcgcctcg gacgccgtcg tcggggagac cctcggggcc
     3001 gtagggcacg taggctaccg acgagccgcc gccgacgtcc ctgacgacga cgagcacgtc
     3061 gcccgcgggc tcgcccgagg cgtccaggtc gaaagccatg gccgaccagc cgagcatccg
     3121 tttgacgcgg gcccagaggg tcgtctgctg tacgagaggg gtcgactcga gggccgatgc
     3181 cggcttcggc ttgatacgga tgtccaaacg cagctccaag gatggtattc gcgcgagacg
     3241 acgcgcaggc gagtagagaa taagtatagc ccgtagcgag cccccggtac aaggctcgcc
     3301 cgccgcgctt gtcaagaatg ggcgctcgtg cgacgatgtt ccgatggatt cgagcgatca
     3361 gccccgcgac ggtcgcttcc cgcgcctggc cgtcgacctg ggcatcatcg agaacaacgc
     3421 ccgcttcgtg cgccaggcct gcgcccgctc ggggataggg ctggtggccg tcgtcaagag
     3481 cgtcggggcc cggattccga tcgtggcggc tatggccagg ggcggttgcc gctctttcgc
     3541 gagctccagg ctcgagcagc tggccctcct acgcgaggcg gccctcgatc ccggctccgg
     3601 cgtaccgccg gacgcgacga cgggcctcct ccgcgcgacc ggtcgctcgg aggccgccgc
     3661 ggccgtggcg ctcgcggact ggagccttca gtcggaggct gccgcgctcg aggcgacggc
     3721 ggacgcggcc gaggcccggg gcgtcaggca cggcgtcacc ctgatgctcg acctcggcga
     3781 cctgcgcgag ggctggttcg ataggcagga gctcgtcgcg gcggcgctgc gggtcgagcg
     3841 cgggttaccg tccctgcgcc tgcgcggcat cgggaccaac ctcggctgct acggggcggt
     3901 tctgccgacc cgggagaatc tcggccggct tctcgaggcg gcgcgcgagg tggaagacgc
     3961 gataggccgg aggttggacg tggtctcggg cggggcaacg acgagcctgc ccctcgtcgc
     4021 ccgcggccgt atgcccgccg gcgtcaacga gctgcggata ggcgaggcga tactctgcgg
     4081 caaggacctg cccctgttcc accgcgtcga gctgcccggc gtacgcggcg acacgataac
     4141 cctgcgcgcc gagctgctcg agaagcacgt aaagccgagc tatccgatag gggcgcgcct
     4201 gcgggacgcg ttcggggcct gtccggaata cgaggaccgg ggtacgcgga ccaggctgct
     4261 cctcggcgtc ggcaagcggg acatcgggaa ccacgagctc ctgaggcctc gcgacggccg
     4321 aatccatatc gtcggctcgt cgagcgacta cctgatctgc gaggcggacg agccgtgcga
     4381 cgacctgcgc tacggctcta tcctggaatt cgacatgctg tacggcgcca tgctgttcct
     4441 ctgcgacgcc aacccgtatc tgtccgtcga ttaccgggcg gccgagggga gccgccccgt
     4501 ggagccctag gctacttgcc gcagcactgc ttatatttct tgcccgagcc gtaggggcac
     4561 ttctcgttgc ggccgacctt cggggtggcc ctgaccacgg tcgcggtctt gacctcgccg
     4621 gagtcgtaga gccacgcgcc gccttcgcgg acgaaacgcg ccgtctcgtg gtgctcttcc
     4681 ttgaggccgt ccatctcgta tcgggcgacg aattcgacga cgccctcatc gtctccgggg
     4741 ccgcctttct cggtcctgac tatcgtcagg cccttccagt cggcgctctc gctccagtcc
     4801 cgcgtcgcct cggcgtcgat gccgtcgtcc tgcacgcacg atgacagaat gtgatcgacg
     4861 gcgcccttgg cgtaggccgt atagcgagat cgcatcagcg cctcggcggt cggggccttg
     4921 gccttgccgg cgatgatggg gccgcagcat tcggcgtaat cgcggcctga gccgcaggga
     4981 cagggttcgc tcatcggtat cctccggagg ctacgttgta gcgtgaaacg gccgtcccag
     5041 gcaagagaag gacatggtct cgcaccggcg gccgcgcatg ggcggccgca caggattgca
     5101 gcggatacgg ccgccgtgta caatgccgcc atgcaagacg cactcatgcg agccaggctg
     5161 gcgtcgcggt cggcatgctc cggcggcacc gtcgcctcgt atatacccgc tctagccgga
     5221 gccgacggct ctgccctcgg gctggccgcc gtggatgccg acggccatgt ctacgaggcc
     5281 ggcgactcga ggagccgctt cacgatccag agcatctcca aggtcctggc gctcgcctac
     5341 gtgatgacgg agctcggcga gggggccgtc ttctcccgcg tcggcaagga accgaccggc
     5401 gaccccttca actcgatcat caggctcgag acgagcgccg gccgcaagcc cttcaacccg
     5461 ctgatcaacg ccggggccat tgtcgtgtcg tcgctcatgc cgggcgacgg ccccgcgaac
     5521 aagggggagc gtttccgctc cttcgtcggc ggcttggtcg gccgaacctc ggtggagcgg
     5581 gacgatgccg tctaccagtc cgagaaatcg actgccgcgc gtaaccgctc gattgcctgg
     5641 ttcctcaagg agctcgggct gctcgagggc gaggtcgagg acgcgctcga cgtgtacttc
     5701 aggcaatgcg ccgtggctat ggacgcggtc gagctggccc gggtcggagc tatcctcgcg
     5761 ctggatggcg tcgacccggt gagcggctcc agggccctgg ccgcgcggac ggccagggtc
     5821 tgtaagagcc tcatggtcac ctgcggcatg tacgacggct cgggcgagtt cgccgtcgac
     5881 gtcggcgtgc cggccaagag cggcgtcggc ggcgggatca tggccgccgt gcgcgaccgc
     5941 ctggggctcg gcgtctacgg cccggccctc gacgagcggg gcaactccgc cggcggcctc
     6001 gccgcgctca ggctcctatc cgacgaactg gacctgcggg tgctatgatc cgccgccgcc
     6061 tgcgaccctc ggcgtacgat cggtttggag actgccgaac cgagccaggg ctgatacggc
     6121 aggcgcgcca tagtatccga gccagataga acgttaaaaa gaacggccgc cccgaggata
     6181 cggggcggcc acctagccgg agcgagtcga gcccggcccc ggtcagaacg ggttcgccta
     6241 ctgcctaccg atggtcgcca ccatcacggc cttgatcgtg tgcaggcggt tctcggcctc
     6301 gtcgaagacc acggagtggc ggctgcggaa gacctcgtcg gtcacttcct gctccatgac
     6361 gccgtgctcc ttgccgacct ggcggccgac ctcggtctcc tggtcgtgga aggccggcag
     6421 gcagtggagg aagatgcagt ccgggttgcc ggtcttcctg atgaagtcca tcgtcacgcg
     6481 ataagccgcg agctgcttga tgcgctcggc gaacttggct tcctcgccca tcgacaccca
     6541 gacgtcggta tacagcgcgt cggcgccctt cagggcctcg tcggccttgg agatgtcgaa
     6601 gaattcgatc acggcgccgg tcgatttggc cacgtcccgc atctcggcgg tcagtttctc
     6661 gttcggccac agctccttgg gggccagcgc gacgaagtgc atgcccatct tggcggagcc
     6721 tatcatcagg gagttgccca tgttgttgcg ggcgtcgccg cagtagacca gcttgacctt
     6781 gttgagcggc ttcttgacgt gctcctcgat ggtcaggagg tcggccagga tctgggtcgg
     6841 gtggaagtcg
//
LOCUS       DAOPHV010000062         6824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_14313_length_6824_cov_4.196779, whole genome shotgun sequence.
ACCESSION   DAOPHV010000062 DAOPHV010000000
VERSION     DAOPHV010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14313_length_6824_cov_4.196779"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PLQ29_02760"
     CDS             complement(<1..747)
                     /locus_tag="PLQ29_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPG85596.1"
                     /translation="MSDDMYVCPACGETYSLASMTFRCRCGGLLDLAPFEPRFDPRSS
                     GGERSIFRYASSMPPFSGLEEAWESARSRVTMGEGLTPTVPFGPAEPGLLVKVDYAMP
                     TLSFKDRGAAVLVAAALVAGARRLIQDSSGNAGAAVAAYATRAGLGCDIYTPASTSQR
                     KIAQIEAYGARAVLVPGSRKDTAAAALEAAEFGARDGSAFYASHVYNPLFYQGTKTYV
                     YELFETLGGLPDTLYAPLGNGTLVLGMYHAL"
     gene            complement(805..1068)
                     /locus_tag="PLQ29_02765"
     CDS             complement(805..1068)
                     /locus_tag="PLQ29_02765"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HPG85597.1"
                     /translation="MSGRYGYVLFHTSAAAFRAEKASRESCASASDTLSGARLVPTPR
                     ALSSDCGVSLRFDLAGTDDGGAERAMRVLLDERGIPFDRIAEA"
     gene            complement(1065..2258)
                     /locus_tag="PLQ29_02770"
     CDS             complement(1065..2258)
                     /locus_tag="PLQ29_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPG85598.1"
                     /translation="MGGEGRIVYLDNAATSWPKPACVSDAMAACMRESGGNPGRSGHR
                     LSIAAGRVVAEAREAVAEALGAGDPLRVAFAANATAAINAALLGVLRPGDRVVADSLA
                     HNAVARPLTELGRNGVAVEWIRCGRDGSVDLGHAEALVRGRGGRAARMLIVTHGSNVC
                     GSLAPLGELAAICARHGTTLLVDAAQTAGVEYIGLDSLGDAMVAFTGHKAMLGPGGTG
                     GLALSSRADIERVSPVIRGGTGSASDSEDQPAFMPDRLEAGTTNAPGLAGLAAGLAWI
                     GANGGRESIREHERRLTENLVSGVAALPGAIVYGPEPGRPRCAVMSFTLDGWSCSDLA
                     LALDERFGVLCRVGLHCAPRAHRTLGTFPDGTARFAPGPFTTEADVAYALGALAELAS
                     EGGRS"
     gene            complement(2260..2496)
                     /locus_tag="PLQ29_02775"
     CDS             complement(2260..2496)
                     /locus_tag="PLQ29_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HPG85599.1"
                     /translation="MSDSDDIVKVDARGLACPEPLMLAKSAMNRACGKTVEVTVDTET
                     AKGNIVRLAGREGRDVVTLAEGGAIIVRISGKKA"
     gene            complement(2765..3871)
                     /gene="yedE"
                     /locus_tag="PLQ29_02780"
     CDS             complement(2765..3871)
                     /gene="yedE"
                     /locus_tag="PLQ29_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HPG85600.1"
                     /translation="MKTKSGFMISKAGIIVTGAVIGVLAALLVKLGNPANMGVCVACF
                     ERDIAGALGLHRAAAVQRLRPEIPAFILGSFVASLLAREWKPRTGSAPLARFVLGAFS
                     MIGALVFLGCPWRAWLRLAGGDMNAIFGLLGLFAGVLIGIAFLKRGYSLGRNHPAPAA
                     LGFVMPAIALGLLALAVVMPLFGRGADGQAIGPIFTSASGPGSMAAPIAVSLAVGLVI
                     GYLAQRSRFCTVGAVRDLVLFRDPHLMLGLLSLFAAALVTNLALGQFKLGFAGQPVAH
                     ADGLWNFGGMALSGLAFTLAGGCPGRQLFLSGEGDADAGVFVLGMITGAAFAHNFNLA
                     SSAAGPSPYGPAATIVGLVVVAVIGLAFREKAAE"
     gene            complement(3943..5022)
                     /gene="selD"
                     /locus_tag="PLQ29_02785"
     CDS             complement(3943..5022)
                     /gene="selD"
                     /locus_tag="PLQ29_02785"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4969..4971),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPG85601.1"
                     /translation="MMDKDGIRLTSLCSAAGUAAKMGPEALAQVLRPLAGLVPEQRAS
                     SLISGLDEGDDAAVYRIDDDTCIIFTADFFTPIVDDPYDFGAIAAANAMSDVYATGGE
                     PILALNLAAFPPDLPPSAIAEIVRGGAETASKAGCAVAGGHTIMDPEPKYGMAVVGIA
                     HPDRVTLKSGARPGDAIVLTKALGTGAITTAAKEGSCPPEVLAGAVSSMRLLNRDAMR
                     VVAAHGVRACTDVTGFSLAGHALELADKGGVGLRLYARALRYLDGAAELAAAGFLPGG
                     AFRNRSFYGPAVAFEAGLDEAFRGLFFVPETSGGLLAAVPREEAEACLAELIAAGAAA
                     AIVGVVTERGQGPAMTVVDANTDGG"
     gene            5191..5279
                     /locus_tag="PLQ29_02790"
     tRNA            5191..5279
                     /locus_tag="PLQ29_02790"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5225..5227,aa:Sec,seq:tca)
     gene            5281..6699
                     /gene="selA"
                     /locus_tag="PLQ29_02795"
     CDS             5281..6699
                     /gene="selA"
                     /locus_tag="PLQ29_02795"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HPG85602.1"
                     /translation="MDISERASGIPQVERLLADDEIRAFFPLVSRPLAARTVSAALAA
                     ERSRALADPAYRADPTVCRASVLSALRALELKRLRPVMNGTGIVLNTNLGRSPVSAEA
                     WDDARGANVGYAAVEYDLAAGKRGSRGGLCGELAATLAGAEAALVVNNNAAGVLLALS
                     AIAKGRDVLVARGEQVQIGGGFRVPDVLELAGARFVEVGTTNIVDTDDYRRAVGSDTA
                     CALIVHTANFALRGFCSKPSPAEIVTAMPDGLPVIVDQGSGCYFDGLPGEARVRSYID
                     AGCTLVCFSGDKLLGGPQAGIIAGRADLIAKLARHPLYRAFRPGKTVLSLLERVLVER
                     LNGEGGPAQAAVSYPTDELKKLARRIRGKLPKGCAVIVDTKAAAGGGSGPDEVFDSIG
                     LSIASPSSAGALLAALRKAPTPLVGLVRDERVVIDMITLVGEDPAAVTETLLWGLERS
                     ASLRARADRLSAATPPNAGHQA"
BASE COUNT         1144 a         2417 c         2243 g         1020 t
ORIGIN      
        1 gagcgcgtgg tacatcccga gcaccagagt cccgttgccg agcggcgcgt agagcgtatc
       61 gggtaggccg cccagagtct caaatagctc gtacacatag gtcttggtcc cctgatagaa
      121 aagagggttg tagacgtggc tcgcgtagaa ggccgagccg tcgcgagcgc cgaactcggc
      181 cgcctcgagc gccgcggcgg ccgtatcctt gcggctaccg ggtacgagta cggccctggc
      241 gccgtacgcc tcgatctgag ctatcttgcg ctgagaggtg gaggcaggcg tatagatgtc
      301 gcagccgagg cccgccctgg tcgcgtaggc ggccaccgcc gccccggcgt tgcccgagct
      361 atcctggatc agcctgcgcg ccccggcgac gagggcggcc gccaccagca cggcggctcc
      421 gcggtccttg aacgacaggg tcggcatcgc gtagtcaacc ttgacgagca ggccaggctc
      481 ggccgggccg aagggtacgg tcggcgtgag cccctccccc atggtcacgc ggctccgggc
      541 cgattcccaa gcctcctcca ggccggagaa gggaggcatg gaggaggcat agcgaaagat
      601 ggaccgctcg ccgccggaag atcgcgggtc gaaccgaggc tcgaacggcg ccaggtcgag
      661 gaggccgccg caacggcacc tgaaggtcat cgatgccagg gaataggtct cgccgcaggc
      721 gggacagacg tacatatcgt cgctcatgcc gtcgattatc gccgatcacg gcgactatag
      781 gcaatcgaat tacgaccggc gacgttacgc ctcggctatc ctgtcgaagg ggatgccgcg
      841 ctcgtccagc aggacgcgca tcgcccgctc ggcgccgcca tcgtcggtcc ctgccagatc
      901 gaaacggagc gagacgccgc agtcgctcga cagcgccctc ggcgtcggga ccagcctagc
      961 cccggacagc gtatcggacg cggacgcgca ggactcgcgg gaggccttct cggccctgaa
     1021 ggccgcggcg ctcgtatgga acagcacgta gccgtaacgt ccgctcacga gcggccgcct
     1081 tcggacgcca actcggccag ggcgccgagc gcgtaagcca cgtcggcttc agtcgtaaac
     1141 ggccccgggg cgaacctggc cgtgccgtcc gggaaggtac cgagagtacg gtgcgccctc
     1201 ggcgcgcagt ggaggccaac gcggcacaaa acgccgaagc gctcgtcgag cgctagcgcc
     1261 agatcagagc agctccagcc gtccaaggtg aacgacatga cggcgcatcg gggcctaccg
     1321 ggctccgggc catagacgat ggcgccgggc agcgcggcga cgccggagac gaggttctcg
     1381 gtcagacgac gttcatgttc ccggatcgac tcccggccgc cgttcgcgcc gatccaggcc
     1441 aggccggcgg cgagcccggc gagccccggc gcgttcgtcg tgcccgcctc gagtcggtcg
     1501 ggcatgaagg ccggctggtc ctcgctatcg gacgcgctgc cggtaccgcc ccggatgacc
     1561 ggactgacgc gctcgatatc cgcgcggctc gacagggcca gcccgcccgt gccgcccggg
     1621 ccgagcatcg ccttgtggcc cgtgaacgcg accatggcgt ctcccagcga gtcgagcccg
     1681 atatactcca ccccggcggt ctgcgccgcg tcgacgagca gggtcgtccc gtggcgtgcg
     1741 catatcgcgg ccagctctcc gagcggggcc agcgagccgc aaacgttcga accgtgcgtg
     1801 acgatgagca tcctggctgc gcggccgccc cgaccgcgga caagcgcctc cgcgtggccg
     1861 aggtcgaccg agccgtctcg gccgcagcgt atccactcga ccgccacgcc gttccgtcct
     1921 agttcggtca gcgggcgcgc cacggcgtta tgggccaggc tatcggcgac gaccctgtct
     1981 cccggccgta ggacgccgag cagggccgcg ttgatcgccg ccgtggcgtt ggccgcgaac
     2041 gcgacgcgca acggatcgcc ggcgccgagc gcctcggcta ccgcctcgcg ggcctccgcc
     2101 acgacgcgcc cggcggctat ggataggcga tggccggatc ggcccggatt gccgcccgac
     2161 tcgcgcatgc acgcggccat ggcgtccgag acgcaagctg gcttcggcca gctcgtcgcc
     2221 gcgttgtcca ggtagacgat ccgaccttcg ccgcccacgc tacgccttct taccgcttat
     2281 gcggacgatg atagccccgc cttcagcgag ggtcaccacg tcgcggccct cgcggccggc
     2341 caggcgcacg atgttaccct tggcggtctc cgtgtcgacc gtcacctcga cggtcttgcc
     2401 gcacgccctg ttcatggccg acttggccaa catcaacggt tccgggcaag ccaaccccct
     2461 cgcgtccacc ttcacgatat cgtcactgtc agacatacat actcctttat ataaggacaa
     2521 acgaagagga taagagaaaa agagtttaag aaggagtaaa caagatttaa aaagaggtat
     2581 gcaagcgcgc gcctcgtata ctccttgctc tcttctccgt acccctaatt ccttttcttc
     2641 tcttaccgat tcttctctta ccgacctctt attctctttt cctcttcgcc gtcttacgct
     2701 acacccaaac ttcttctatt ctctttcctg tctcttatcc tcgtatcctc ttcgccgtct
     2761 tacgctactc ggccgctttc tcacggaagg ccaggcctat gacggctacg acgacgaggc
     2821 cgacgatggt cgccgccggt ccgtagggcg acggaccggc ggcggagctg gcgaggttga
     2881 agttatgagc gaaggccgcg ccggttatca tgccgagcac gaacacgccg gcgtcggcgt
     2941 cgccctctcc ggataggaag agctgccgcc cggggcatcc gccggccagc gtgaaggcca
     3001 agcccgatag cgccatgccg ccgaagttcc acagcccgtc agcgtgggcg accggctggc
     3061 cggcgaagcc gagcttgaac tggccgagcg ccaggttggt aacgagagcc gcggcgaaca
     3121 gcgacaggag gccgagcatc aggtgggggt ccctgaagag cacgagatcg cgcaccgctc
     3181 cgaccgtgca gaagcgcgag cgctgggcca ggtagccgat gaccagcccg accgccagcg
     3241 atacggcgat aggcgcggcc atcgaaccgg ggcccgaggc gctcgtgaag atcggaccga
     3301 tcgcctgccc gtcggcgcct cggccgaata gcggcatgac taccgccagg gccagcaggc
     3361 ccagggctat agccggcatg acgaagccga gggcggccgg cgccggatgg ttacgcccga
     3421 ggctgtagcc gcgcttcaag aacgctatgc cgatcagtac gcccgcgaac aggcctaaga
     3481 gcccgaagat cgcgttcatg tcgccgccgg ccagccggag ccaggcgcgc caggggcagc
     3541 cgaggaacac gagcgcgcct atcatggaaa aggcgccgag cacgaacctg gccagcgggg
     3601 cggaaccggt cctcggcttc cattcgcggg ccaggagcga ggcgacgaag ctacccagga
     3661 tgaaggccgg gatctcgggc ctaaggcgct gtaccgcggc ggcgcgatgc aggccgagcg
     3721 ccccggcgat gtcgcgctcg aagcaggcga cgcagacgcc catgttcgcc ggattgccga
     3781 gtttgacgag cagcgcggcc aggacgccta tcacggcccc ggtcactata atgcccgcct
     3841 tcgagatcat gaatccagat ttcgttttca actgtatcct ccttgcataa gcgacgcgcg
     3901 gctatccggc cgcaccccgc ctagcgagga gcgtcggccg gatcagccgc cgtcagtatt
     3961 cgcgtcgacg accgtcatcg ccgggccttg cccccgttcg gtgacgacgc cgactatggc
     4021 cgccgcggct cccgcggcga tcagctcggc gaggcaggcc tcggcctcct cgcgcggtac
     4081 ggcggccagg aggccgcccg aggtctccgg cacgaagaac aggccccgga aggcctcgtc
     4141 caggccggct tcgaacgcca cggccggccc gtagaacgag cggttcctga aggcgccacc
     4201 cggcaggaac ccggccgctg ccaattcggc cgccccgtcc aggtaccgta gcgcccgtgc
     4261 gtacagcctg agcccgacgc cacccttgtc ggccagctcg agcgcgtggc ccgccagcga
     4321 gaagccggtc acgtcggtac aggcgcgcac cccatgggcc gcgacgacgc gcatcgcatc
     4381 ccgattgagg agccgcatcg aagaaaccgc gccggccagg acctcgggag ggcatgaacc
     4441 ctccttggcc gccgtcgtga tcgctccggt gccgagcgcc ttggtcagca ctatcgcgtc
     4501 gcccggccga gcgcccgact tgagcgtcac gcgatcggga tgggcgatgc ccacgacggc
     4561 catgccgtac ttgggctccg ggtccatgat cgtgtggccg cccgcgacgg cgcagccagc
     4621 cttcgacgcc gtttccgccc cgccgcgcac gatctccgct atggccgagg gcggcaggtc
     4681 aggcgggaac gccgccaggt tgagggctag gatgggctcg cctccggtag cgtacacgtc
     4741 ggacatggcg ttggccgccg ctatcgcgcc gaaatcgtac ggatcgtcca ctatcggcgt
     4801 gaagaaatcg gccgtgaaaa taatgcatgt gtcgtcgtcg atccggtaca ccgcggcgtc
     4861 gtcgccctcg tccagcccgg aaataaggga tgacgcgcgc tgttccggca cgagaccggc
     4921 taaaggacgc agaacctgcg ccagggcctc ggggcccatt ttcgccgctc acccggcagc
     4981 ggagcacaac gacgtcagcc tgatgccgtc cttatccatc atccctcctt tccggcgaat
     5041 tacccactcg atgggtatat accgcgacat gcgagcaagc ctatcagcga ttcggcggct
     5101 tggcaagcgc aagcgccgga tccaacgtcg gacgaacgta tacactcgcg ttgacaacgc
     5161 cgtgggcgtc ccgtagtatg cagacgacag ggaagctgat ggggtctggt ggctcccgcg
     5221 gtcttcaaaa ccgttggctg cgcctcgcgc ggtggtgggt tcgattcccg cctcttccgt
     5281 atggacataa gcgaacgcgc atcgggcatt ccacaggtcg agcgcctcct ggccgacgac
     5341 gagatacggg cgttcttccc gctcgtatcg cgacccctag ccgcgcgcac ggtatcggcc
     5401 gcgctggccg ccgagcgctc tcgcgcgctc gccgacccgg cctaccgagc cgacccgacg
     5461 gtatgccggg cctcggtcct atcggcgctc agggcgctcg agctcaagcg cttgcgaccc
     5521 gtcatgaacg gtacgggcat cgtgctcaac acgaacctcg gccggtcccc cgtgtcggcc
     5581 gaggcctggg acgacgcgcg cggcgccaac gtcggctacg ccgccgtcga atatgacctg
     5641 gcggccggaa agcgcggtag tcgcggcggc ctctgcggcg agctggccgc gacgttggcc
     5701 ggcgccgaag ccgcgctcgt cgtcaacaac aacgccgcgg gcgtcctcct cgccctgagc
     5761 gccatcgcca agggccgcga cgtactagta gccaggggcg agcaggtaca gataggcggc
     5821 ggcttccgcg tgccggacgt gctcgagctc gctggcgccc gcttcgtcga ggtaggcacc
     5881 acgaacatcg tcgatacgga cgactatcgt cgcgccgtcg gctcggatac cgcctgcgcc
     5941 ctgatcgtgc acacggccaa cttcgcactc cgaggcttct gctcgaagcc aagccccgcc
     6001 gagatcgtga cggcgatgcc ggacggcctg ccggtcatcg tcgaccaggg ctccggctgc
     6061 tacttcgacg gccttccggg cgaggcgcgc gtccgctcct acatcgatgc aggctgcacc
     6121 ctggtctgct tctccggaga caaactgctc ggcggccccc aggccggcat catcgccggc
     6181 cgcgccgacc tcatagcgaa gctcgccagg catccgctgt accgcgcctt caggccgggc
     6241 aagaccgtcc tgtcgttgct cgagcgcgtc ctcgtggagc gcctcaacgg cgagggcggc
     6301 ccggctcaag cggcggtatc ctatccgacc gacgaactca agaagctggc ccgtaggata
     6361 cgaggcaagc tgcccaaggg ttgcgccgtc atcgtcgata ccaaggcggc ggccggcggc
     6421 ggctccggcc ccgacgaggt cttcgactcc atcggcctgt ccatcgcctc gccgtccagc
     6481 gccggagcgt tgctcgccgc cctgcgcaag gcgccgacgc ccctcgtcgg cctcgtccgc
     6541 gatgagcggg tcgttatcga catgatcacg ctggtgggcg aggatcccgc ggccgtcacc
     6601 gagacgctcc tctggggcct cgagcgtagc gcgtcgctac gagccagggc cgaccgcctg
     6661 tcggccgcga cgcccccgaa cgcgggccat caggcgtagc gtcgtgcgga tcatcggcac
     6721 cgccgggcac gtggaccacg gtaagaccgc cctgatccgc gcgatgaccg gcatcgacgc
     6781 ggatcgcctg cccgaggaga aggcccgcgg catgaccacc gacc
//
LOCUS       DAOPHV010000063         6806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_14377_length_6806_cov_3.647312, whole genome shotgun sequence.
ACCESSION   DAOPHV010000063 DAOPHV010000000
VERSION     DAOPHV010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14377_length_6806_cov_3.647312"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PLQ29_02800"
     CDS             complement(<1..889)
                     /locus_tag="PLQ29_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPG85603.1"
                     /translation="MPEPPNRVALVYGVSTYIEGGSSVNLSLTDDDARAMGAMLSAKG
                     WSVTTRVADSSVATTNEQASRDRIEADIAALAGFDGIVLFYYSGHGAYDVREESAICP
                     YGSIVASGEGYFFDLGAMITASELRAMFETAGLGRVVIVLDSCNSGGFVAAGATTDAI
                     PDSYGVYDDDIAYTWFVGAFGDAVAAFSYDAEPGYVVLSAAGSDEFSWESGDHGIFTR
                     YLLEAGGANSKAADRDGDGYLSTTELYRYALLGIEAKWNRIYDETPTLRWQLYAADYW
                     DDYLPHLSGTTSEYALWSVG"
     gene            complement(988..1569)
                     /locus_tag="PLQ29_02805"
     CDS             complement(988..1569)
                     /locus_tag="PLQ29_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85604.1"
                     /translation="MRVIRLTILASLLASTAAFAAPAPAVSFLASVAPGVALSSGMGS
                     GTAPGAAVSAELGARIGGDRLAAMIRAGVVKQAASAYTDVWTRYRGFSGLSLAAGPNL
                     RLGLFEFGVLAGGALARYDLSYSYFAFPYIEPRASYRAAHLDDRFSLGIGLSLPVYLR
                     ADALSLGFRAVVELRYESPHQERPSTPSAESAP"
     gene            1635..2045
                     /locus_tag="PLQ29_02810"
     CDS             1635..2045
                     /locus_tag="PLQ29_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85605.1"
                     /translation="MYRIERDSMIKTRIAASTALAIVAATTMALAACSVDKNPRVELD
                     ETPIISGGLGWGVVSLSYARLMLEPSLDAADSGTCRRGDVGELLARTRVAKGRDGGVW
                     YRARFDGGTGWLHESALAVFRTENEARNAVEARP"
     gene            2042..3610
                     /locus_tag="PLQ29_02815"
     CDS             2042..3610
                     /locus_tag="PLQ29_02815"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HPG85606.1"
                     /translation="MSLPELGTFLIPPLVGSVIGLFTNWLAIKMLFRPLAEKRLLGLR
                     VPFTPGILPRERERMARSLGETVAVDLLDERTVSERLRSQAFKDAVRSAAYGLGKKAL
                     EAKPGDLVSGLDGKTGKALRDVALELLSGVASSGVFARSVSAGAKAAVAASNAVKLSD
                     VVPGSAVGALAEALTGPEGGARTAAFLAEALMDAFEMAADEGKAVSDIVGADRLVALA
                     EKAVLAAYPSVIEATVGMMADKKVAASMEKAAARVIKRALDRFNSVQRFFISLGQYDK
                     AIMENMPATIADLGDSMRGILSEPSTKAAVLAKAAAAARGFAARPLAGFGFMRLAESR
                     EAARLSLEEALRDALAPLEPRAIAGMISGMLDRASLGEILEALPGVAERIGPALARWL
                     AGLSSKRPPDGSAGGAIAAAFFAAFASSFGSEASGAPLSETVSIDDEALGAIAASAAD
                     GLSELAAAQSGEVIRSIDIRSLVVDKIDSLDMIEVERMILKVVDKELGAITMFGGILG
                     AVIGLFQSLLFMLR"
     gene            3638..4669
                     /locus_tag="PLQ29_02820"
     CDS             3638..4669
                     /locus_tag="PLQ29_02820"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPG85607.1"
                     /translation="MDDDRTILIVDDSRAFALAAAEMVEGEGYDVHLAHSATDALEAA
                     ERVRPRVVFLDVVLPDGEGYEVCSALKRTFMHRPLQVVMMSAVYDAKRMDRLLATGAD
                     DFIKKPFEALEFQLRLKAAHIRLQAQTKLMQEREYYRQAVRQEENLTIKLLDRQLGLK
                     ETLADLESKKAGLERENTGLAAAARFDVLTSLLNRHSLNARLELEARRSDAEDARLSG
                     LMVDVDRFKAVNDSYGHLVGDEALRSVGDALRACLRKEDFAGRYGGDEFFAILPGSGI
                     EAALAIAERIRSTIAAAEIHLGGVRVRLSASIGAAERRKGDSVAEWIGRADAAMYRAK
                     NAGRDRVMS"
     gene            complement(4666..6012)
                     /locus_tag="PLQ29_02825"
     CDS             complement(4666..6012)
                     /locus_tag="PLQ29_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPG85608.1"
                     /translation="MKFEGSDQVYAYLNRFLNFERKLQPTEYRLDRMTRLKEMFGRPD
                     EGYRVYHVAGSKGKGSTAAMLAAMLSASGRRTGLYTSPHLLSFKERIAIDGEPVDDAT
                     LVACAEELSDGVDAERADGYPGGEEPTYFELLTMLAFLCFRRAGCTDAVFEVGLGGRL
                     DSTNVVRPVVCLITPIELEHTELLGDTIAEIAGEKAGILKPGVPAFTSATKPDALRVI
                     RERARGLGCALTVLDEAAELSGVTISERGTEADVRWLAPGASADRLPARLATPMIGSV
                     QLRNAALAALAGASIGLTRDDIVRGLSAARLRARFEILDAGTRVILDGAHTDDSIRAC
                     VDDFSSLFPGGGSLLFGCAADKAAGAMAESLRSAFTEVVITRPGTFKESDTAALERTF
                     IEHGFEVRRIDDTAEAVRTAIDGAIAAGLPLLVTGSFYLCAYAAAELEARRARHQA"
     gene            complement(6009..>6806)
                     /locus_tag="PLQ29_02830"
     CDS             complement(6009..>6806)
                     /locus_tag="PLQ29_02830"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85609.1"
                     /translation="ANGLDYGATVLPPWSTIDDALAFCDGHAALVRGDAPQALYEALE
                     SICPVVVNGAAATWLAGDKAASAERFAAIGARHPRTVAIELCSEEPPLEPPFVLKPRF
                     GKMGRGVELVESLEAWQAIHGRLRDGTGLEAGGYVAQDYVEASRGRDLRFFFARFAGM
                     GDDDEIPPCVSVLREGSGLASNAHAGGSMVPFAAPGDLVAEARRVFVDSGLIYGTVDF
                     LFADETGSSFFVCETNACPGFEALEACTGLDAAGAILRSVSFAGGEP"
BASE COUNT         1174 a         2213 c         2317 g         1102 t
ORIGIN      
        1 ccccgacgga ccagagcgcg tactcgctcg tcgtgcccga cagatggggc aggtagtcgt
       61 cccagtagtc agcggcgtat agctgccacc gtaaagtcgg cgtttcgtca tagatacggt
      121 tccacttggc ctctatgccc agaagcgcgt agcggtacag ttccgtcgtc gagaggtagc
      181 cgtcgccgtc gcggtcggcc gccttggagt tcgcgccgcc cgcctcgagc aggtagcgcg
      241 tgaagatgcc gtggtcgccc gattcccagg aaaattcgtc ggagccggcg gccgatagca
      301 cgacgtagcc gggttccgcg tcataggaga aggccgcgac ggcgtcgccg aacgcaccca
      361 cgaaccaggt ataggctata tcgtcatcgt agacgccgta cgagtcgggg atagcgtcgg
      421 ttgtggctcc ggcggcgacg aagccgccgg aattgcagct gtcgagcacg atcacgacgc
      481 gaccgagccc cgcggtctcg aacatggcgc gtagctccga ggcggtgatc atcgcgccca
      541 ggtcaaagaa gtagccctca ccggatgcta cgatagaacc ataggggcag atggccgatt
      601 cttcgcgaac gtcgtacgcg ccatgaccgg aataatagaa cagcactata ccgtcgaagc
      661 cggccagcgc ggcgatgtcc gcctcgatcc gatcgcggct cgcctgttcg ttggtggtgg
      721 ctacgctcga atcggccacg cgcgtcgtca cggaccatcc cttggccgac agcatcgcgc
      781 ccatggccct ggcgtcgtcg tcggtcaggc tcaggttaac gctcgagccg ccctcgatgt
      841 acgtagacac tccgtagacc agcgcgaccc ggttcggcgg ctctggcatg gaacaggcaa
      901 gcaggctcgc cccggcggcc aaggttgcgg cggccgctcc gaacgcagca cgaagctcgt
      961 ggcccgcgcc gcgtcccagg cgccgactca cggcgcactt tccgcggacg gcgtcgacgg
     1021 ccgctcctga tgcggcgatt cgtagcgcag ctccacgacg gcccggaagc cgagcgacag
     1081 ggcgtcggcg cgcaggtaca ccggcagaga caggcctatc ccgagcgaga atcggtcatc
     1141 gagatgggcc gcgcggtacg aggctctcgg ctctatatag ggaaaggcga agtacgagta
     1201 cgacaggtcg tagcgggcga gcgcgccgcc agcgagtacc ccgaactcga agagaccgag
     1261 gcgaaggttc gggccggcgg ctaggctcag tccggagaag cctcggtagc gcgtccagac
     1321 gtcggtatag gccgaggcag cctgcttgac gacgccggcc cggatcatcg cggctaatcg
     1381 gtcgccgccg atacgagcgc ctagctcggc cgagaccgcg gcgccggggg cggtgcccga
     1441 gcccatgcct gaactcaggg ccacgccggg tgctactgag gctaagaacg atacggccgg
     1501 agcgggcgcc gcgaaggccg ccgtcgaggc caggagcgaa gctagtatgg ttaatcgaat
     1561 aacgcgcata gatgggcttt aacatgcctc gaaatttgga taaattcaac cagtacccaa
     1621 atcgactcag gttcgtgtac cgaatagaaa gggattcgat gattaaaacc cgtatcgccg
     1681 cttccaccgc cttggccatc gtcgccgcaa cgacgatggc gcttgcagcc tgctcggtcg
     1741 ataagaatcc gcgcgtcgag ctcgacgaga cgcctatcat atccggcggc ctcggctggg
     1801 gcgtcgtctc gctgtcctac gccaggctga tgctcgagcc ttcgctcgac gcggctgact
     1861 cgggcacctg ccgacgcggc gacgttggcg agctattggc caggacgcga gtagccaaag
     1921 gccgcgacgg cggcgtctgg taccgcgctc gcttcgacgg agggaccggc tggctgcacg
     1981 aatcggcgtt ggctgtgttc aggaccgaga acgaggccag gaacgcggtg gaggcgaggc
     2041 cgtgagcctt ccggagctgg gcactttcct gatcccgccg ctggtaggtt cggtcatcgg
     2101 cctgttcacc aattggctgg cgatcaagat gctattcagg ccgctggctg aaaaacggct
     2161 cctcggcctg cgcgttccgt ttacgccggg catacttcca cgtgagcgag agcgtatggc
     2221 ccgctcgctc ggcgagaccg tcgcggtcga cctcctcgac gagcgcacag tctcggaacg
     2281 gctgcgtagc caggcgttca aggatgccgt acgctcggcg gcctacggac tcggcaagaa
     2341 ggccctcgag gccaagccgg gagacctcgt atcggggctg gacggcaaga ccggaaaggc
     2401 cctgcgagac gtagcgctcg agttgctgtc cggcgtcgcc tcgagcggcg tcttcgcccg
     2461 ctcggtatcg gccggcgcca aggccgccgt agctgcgtcg aatgccgtca agctgtccga
     2521 tgtggtgcca ggctcggccg tcggggctct agccgaggcc ctgactggac ccgagggcgg
     2581 agccaggacc gcggccttcc tggccgaggc gctcatggac gccttcgaga tggcggccga
     2641 cgagggcaag gccgtctccg atatcgtcgg tgccgacagg cttgtcgccc tagccgagaa
     2701 agccgtactc gctgcctatc cgtcggtgat cgaggcgact gtcggcatga tggccgacaa
     2761 gaaggtcgcc gcgtcgatgg agaaggccgc ggcccgcgtc atcaagcgag ccctggaccg
     2821 tttcaactcg gtgcagcgct tcttcatcag ccttgggcag tacgacaagg ccatcatgga
     2881 gaacatgcca gcgaccatcg ccgacctggg cgattcgatg cgcgggatac tgtcggaacc
     2941 ttcgaccaag gcggccgtac tggccaaggc cgcggccgca gccagggggt tcgccgctag
     3001 gcctttggcc ggtttcggct tcatgcggtt agccgagtcg cgcgaggccg ccaggctgtc
     3061 cctcgaagag gcgctccgcg acgcgctggc tccgctcgag ccgcgagcca tagccggcat
     3121 gatctccggg atgctcgacc gggccagcct cggcgagata ctcgaggccc tgcccggagt
     3181 ggccgagcgc ataggacccg ccctggcccg ttggctggcc ggcctatcct cgaagcgccc
     3241 ccccgacggc tcggctggcg gagccatagc ggccgccttc ttcgcggcct tcgcgtcgtc
     3301 cttcggctcc gaggcgtctg gcgcgccgct gtcggagacc gtgtcgatcg acgacgaggc
     3361 gctcggcgcg atcgcggcct cggctgccga tggcctttcc gagctggcgg cggcccagag
     3421 cggcgaggtg atcaggtcca tagatattcg ttccctcgtg gttgataaaa tcgactcgtt
     3481 ggatatgatt gaagtagaac gaatgatcct gaaagtagtg gataaggaac tcggcgccat
     3541 caccatgttt ggaggcatac tcggggccgt catcggcctt ttccagagtc tcctgttcat
     3601 gctgcgatag cgaggggccc cgaccgtgag gtaaaaagtg gatgatgaca ggacgatcct
     3661 gatagtcgac gactcgcgcg ccttcgcgct cgccgcggcc gagatggtcg agggcgaggg
     3721 ctatgacgtg cacctggccc attcggccac cgacgcgctc gaggctgccg agcgcgtccg
     3781 tcctcgcgtc gtgttcctcg acgtggtgct acccgacggc gagggctacg aggtctgttc
     3841 agccctcaag cgaaccttca tgcacagacc gctccaggtc gtgatgatgt ccgcagtcta
     3901 cgatgccaag cgcatggaca ggcttttggc gactggcgcg gatgacttca tcaagaagcc
     3961 gttcgaggcg ctcgaattcc agctcaggct taaggccgcc catatacggc tccaggcgca
     4021 gacgaagttg atgcaggagc gtgagtatta ccgtcaggcg gtacgtcagg aggagaacct
     4081 gactatcaag ctgctcgaca ggcaactcgg cctcaaggag acgctagccg acctcgagag
     4141 caagaaggcg gggcttgagc gcgagaacac cgggctagcc gctgccgccc gcttcgacgt
     4201 gctgacgagt ctcctcaatc ggcacagcct gaacgccagg ctcgagctcg aggcgcgccg
     4261 ctccgacgcc gaggacgcgc ggctgtccgg cctcatggtc gacgtggacc ggttcaaggc
     4321 cgtcaacgac tcgtatggcc atctcgtcgg cgacgaggcg ctacgctccg tgggcgacgc
     4381 gctacgcgcc tgcctccgca aggaagactt cgcggggcgc tacggcggcg acgagttttt
     4441 cgcgatcctg cccggcagcg gcatcgaagc ggcgctggcg atagccgaac gcatacgctc
     4501 gaccatcgcg gccgcggaga tacacctcgg cggagtcagg gtacgcctca gcgcatcgat
     4561 cggcgcggcc gagcgacgta agggcgattc ggtggccgaa tggatcggcc gcgccgacgc
     4621 cgccatgtac agggccaaga atgctggccg cgaccgggtg atgagctagg cctggtgccg
     4681 ggctcgcctg gcctcgagct cggcggccgc gtacgcgcac aggtagaacg aaccggtcac
     4741 caggagcggt agcccggcgg cgatcgcccc gtcgatcgcc gttcgtacgg cctcggccgt
     4801 atcgtcgatc ctccgcacct cgaagccatg ctcgataaag gttctctcga gagcggcggt
     4861 gtcgctctcc ttgaaggtgc cggggcgcgt gatgacgacc tccgtgaacg ccgatcgtag
     4921 cgattcggcc atggctccgg ctgctttgtc ggcggcgcac ccgaacagca gggagcctcc
     4981 tcccgggaag aggctcgaga agtcgtcgac gcaggcccgg atcgagtcgt cggtgtgagc
     5041 gccgtccagg atgacgcggg tgccggcgtc gagtatctcg aagcgggcgc ggagtctggc
     5101 ggcgcttagg ccgcgaacga tatcgtcgcg ggttagccct atcgaggcgc ccgccaaggc
     5161 cgccagcgcc gcgttcctga gctgcaccga gcctatcatg ggcgtcgcca gcctggcggg
     5221 caggcggtcg gccgacgctc ccggagcgag ccaccgaacg tccgcctcgg tgcctcgctc
     5281 gctgatcgtc acgcctgaaa gctcggccgc ctcgtcgagg acggtcagcg cgcagccgag
     5341 cccccgggcc cgttcacgta tgacccggag cgcgtctggc ttggtcgcgc tcgtgaacgc
     5401 cggcacgccc ggcttgagta tcccggcctt ctcgccggct atctcggcta tcgtgtcgcc
     5461 gagcagctcg gtgtgctcca gttctatcgg cgtgatcagg cagacgaccg gcctgaccac
     5521 gtttgtcgag tcgagccggc cgccgaggcc tacctcgaag acggcgtccg tacagccggc
     5581 tcgcctgaag cacaggaagg cgagcatcgt caacagctcg aaataggtag gttcctcgcc
     5641 cccgggatag ccatcggcac gctccgcgtc gaccccatcc gacaactcct cggcgcaggc
     5701 aacgagcgtg gcgtcgtcga ccggttctcc gtcgatggct atgcgctcct tgaacgacag
     5761 gaggtgcggc gaggtgtaga ggcccgtccg ccggcctgag gcgctcagca tggcggcgag
     5821 catggcggcg gtcgaaccct tgcccttgga gccggcgacg tggtagaccc ggtagccctc
     5881 gtcgggccgg ccgaacattt ccttgagcct ggtcatgcga tcgagccggt actcggtcgg
     5941 ctgcagcttg cgctcgaagt tgaggaagcg gttcaggtag gcgtagacct ggtccgagcc
     6001 ttcgaatttc atggttcacc tccggcgaac gatactgacc tgaggatggc gccggccgcg
     6061 tcgagtccgg tacaggcttc gagggcttcg aagcccgggc aggcgttcgt ctcgcagacg
     6121 aagaacgacg agccagtctc gtcggcgaac aggaagtcga ccgtgccgta gatcaggccc
     6181 gagtcgacga agacgcgcct cgcctcggcc acgagatcgc ctggcgcggc gaagggtacc
     6241 atcgaaccgc cagcgtgggc gttcgaggcg aggccggacc cctcgcgcag caccgagacg
     6301 caaggaggga tctcgtcgtc gtcgcccatt ccggcgaaac gcgcgaagaa gaaacgtagg
     6361 tcgcgcccgc ggctcgcctc gacatagtcc tgcgcgacgt agccgccggc ttccaagccc
     6421 gtaccgtcgc ggagccgccc gtggatcgcc tgccaggctt ccagcgattc gacgagttcg
     6481 acaccgcggc ccatcttgcc gaatcgcggc ttgagcacga agggcggttc gaggggcggt
     6541 tcctccgagc acagctcgat ggcgacggtt ctcgggtggc gcgcccctat cgcggcgaag
     6601 cgttcggccg aagcggcctt atcgccggcg agccaggtcg cggccgcgcc gttgacgacg
     6661 acggggcaga tcgattcgag cgcctcgtac agggcttgag gcgcgtcgcc gcggaccaga
     6721 gccgcgtggc cgtcgcagaa ggccagggcg tcgtcgatcg tcgaccaagg cggcaggacc
     6781 gtcgcgccat agtccagacc gttggc
//
LOCUS       DAOPHV010000064         6801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_14395_length_6801_cov_4.586422, whole genome shotgun sequence.
ACCESSION   DAOPHV010000064 DAOPHV010000000
VERSION     DAOPHV010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14395_length_6801_cov_4.586422"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..983)
                     /locus_tag="PLQ29_02835"
     CDS             complement(69..983)
                     /locus_tag="PLQ29_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPG85610.1"
                     /translation="MAIATTKIRIGQSIGTRIALLFGLSTLAVLSALALIIAQQAFTS
                     AEDLSEAYSVQITDARADELAAIVSKYVRFAQDIAIRDVIIGGDYAEVTNALKSMNRV
                     IEQEVAGGFVAWPSGDYVTALGVPGNIADRDYFQDIMSGKTDVSVSNPVISKALGIPI
                     VVVAAAIKRNGRNDGMVGLQIPLDNLSRQIADVKLSDNSVAILVDGDGMVIAHPDPKI
                     VMNLNILKADEIGYEGLSAAGRIMQRGEPVSMRMKRPDGAMLHGFASPIANTPNWSIW
                     KRRSPRAVPTPCCSTPSPSPSKSATRGR"
     gene            complement(1113..2768)
                     /locus_tag="PLQ29_02840"
     CDS             complement(1113..2768)
                     /locus_tag="PLQ29_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG85611.1"
                     /translation="MRSIRTKIVTLAAAAALVVAVCLSIVFINLLRSSVDEQVAALET
                     TLRDDFDLLIKSEIETAVSMLERLAALRDEGSLGDAEARNLAATLLRELRYGEDGYFW
                     ADTPQGDNVVLLGGTSEGTNRMALKDVNGYELVRAIIDAAMKGGGYTDYWFPRAGETE
                     AAPKRGYSKHAPGFNWVIGTGNYTDDIDQVITAKREAAAASLRRALVLIVVIAAVLGG
                     AMIVVAVLLGDRIAKPLVYAAAKVKDLAAGRLDDDFDGRMAAGHDEAGMLVSSLAATV
                     AKLRDVITNVQGASAMMIDGSAKLSDASKQMAAGIAGIAESSQQLSQGSSEQAASAEQ
                     VSASVEQMSANIKQNADNSFQTERISSKAATDAREGLTAVKDTVAAMRQIAERIAIIE
                     EIASQTNMLSLNASIEAARAGEHGKGFAVVASEVGKLAERSKLAAGEISVLSQRSVEI
                     ADKAGALLEDMAPDIQKTADLVQEISVASREQDTGAQQINLAMTQLDSVIQQNASISE
                     EFSATSEEIAGQARLVADTSTELADQAHRLQALVGFFSIHGKA"
     gene            complement(2909..3619)
                     /locus_tag="PLQ29_02845"
     CDS             complement(2909..3619)
                     /locus_tag="PLQ29_02845"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPG85612.1"
                     /translation="MAHVTVEDRKQTIADKVAFRLERLVLGGELKPGDRLPPERELAE
                     RFSASRNGVREAIRRLETLGLLSTEPQSGTYVRDYLRDASFDLILHVMRRADDLDPAL
                     LLDLLEYRMMLMTTAARLAATRDPMACSADLANMAAALSATRDPAEAAELDFRFHDRI
                     VKGAGNVLFRSMQNTSRELHLFYTRRFYSLPGALEATAAQQLAIAKAIGSGDPEASAA
                     AMMTALEYGRRAIERMLG"
     gene            3806..4126
                     /locus_tag="PLQ29_02850"
     CDS             3806..4126
                     /locus_tag="PLQ29_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trp family transcriptional regulator"
                     /protein_id="HPG85613.1"
                     /translation="MNDETGLVSTNLTEMSKILAATADAGLIEDFMYSLFTQAEADEI
                     AKRWALVKRIAEGVPQRAIAAELGLSLCKITRGSRELKKENSSFRRMLELGGYEIVEK
                     KRKA"
     gene            complement(4224..4727)
                     /locus_tag="PLQ29_02855"
     CDS             complement(4224..4727)
                     /locus_tag="PLQ29_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPG85614.1"
                     /translation="MANPIVNFKTSLGSFDIELFEDKSPVTVKNFTEYVKAGFYDGTV
                     FHRVIPGFMVQGGGMTPGLKEKDTNAPIKNEADNGVGNERGTIAMARTSDPHSATCQF
                     FINLVDNKPLNFRSKDQAGWGYCAFGKVTSGMDVVDAIAKVETGNRFPHQNVPKADVL
                     IESASLA"
     gene            4853..5539
                     /gene="pyrH"
                     /locus_tag="PLQ29_02860"
     CDS             4853..5539
                     /gene="pyrH"
                     /locus_tag="PLQ29_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPG85615.1"
                     /translation="MTKVLSLGGSIVAPDVPDAAFVKAFALTARRWLDADAARRLIVV
                     VGGGGPARAWQKAYRDAADAPDADAQDWIGIMATRLNAQLVRSLFLDLCPHEVVTDPT
                     SVAVFGGRVLVAAGWKPGFSTDFDAVVLAERFQADVVVNLSNIAKVYTADPRTDPNAK
                     AIDDISWKDFRAMVGDEWTPGKNVPFDPVASRRAEQLGLRVVCAAGKDLANLEAILDG
                     KPFVGTTIGA"
     gene            complement(5550..6593)
                     /locus_tag="PLQ29_02865"
     CDS             complement(5550..6593)
                     /locus_tag="PLQ29_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG85616.1"
                     /translation="MTTKGVSGATRRALQATIYHLPGAPYGAPRPVARRYFGRRSRAL
                     ALVAAATLASGWLGSCSSAPKRQTAVVERKNEAAEYAALADGFLASGQYSSALQYYGE
                     ALDASLSVDDVAGAVKSRGSLGRAYLAIGGLDDAAREFGDALEDARALGEPALVALCL
                     SNLGELRYASGDRDAADALFAEAEALAAGDEAIAAVVAHNRGVVAMARADYAAAEAYV
                     LRSAAANEKARRWSELGSNRYALASIANARGDLPGALAWAAKALEADKRAENSPGIGA
                     DLEALARLQLKSGNGAAAFDYYRRAFGLWLSLGRADDAERCLAALSGLASSLGKDGYA
                     ARYEALLARLRER"
     gene            complement(6590..>6801)
                     /locus_tag="PLQ29_02870"
     CDS             complement(6590..>6801)
                     /locus_tag="PLQ29_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HPG85617.1"
                     /translation="AVRSLQGIITGLNGEMPKIAGLLNETKAAIKQAQDVLEGVKNNP
                     LIRGGVTERVEQESLYSAMREGSFE"
BASE COUNT         1070 a         2209 c         2286 g         1236 t
ORIGIN      
        1 gatggcctgt tcccagcagt ccgtcggcag cacgccgtgg ccggccagca cgcccatcag
       61 cacgctgttc accgcccgcg cgtcgccgac ttcgacggcg acggcgatgg cgtcgagcag
      121 cacggcgttg ggacagcgcg cggcgatctg cgcttccaga tcgaccagtt cggggtattc
      181 gcgatcgggg acgcgaagcc gtgcagcatc gcgccgtcgg gccgcttcat gcgcatcgat
      241 accggttcgc cgcgctgcat gatccgtccg gcggcggaca ggccctcgta gcctatctcg
      301 tcggccttga ggatgttcag gttcatgact atcttcgggt ccgggtgcgc gatgaccatg
      361 ccgtccccgt cgaccagtat ggcgacgctg ttgtcgctca gcttgacgtc ggctatctgc
      421 ctggacaggt tgtcgagcgg tatctggagg ccgaccatgc cgtcgttgcg gccgttccgc
      481 ttgatggctg ccgcgacgac gacgatcggt atgccgagcg ccttggatat cacggggttc
      541 gagacgctga catcggtctt gccgctcatg atgtcctgga agtagtcgcg gtcggctatg
      601 ttgcccggca cgccgagcgc cgtgacgtag tcgccgctcg gccatgccac gaacccgccg
      661 gctacctcct gctcgatcac ccggttcatg ctcttgagcg cgttcgtcac ctcggcgtag
      721 tcgccgccta tgatcacgtc gcgtatcgcg atgtcctggg cgaagcggac gtatttcgag
      781 acgatggcgg ccagttcatc ggccctggcg tcggttatct gcaccgagta ggcctcggat
      841 agatcctcgg cgctcgtgaa cgcctgttgc gcgatgatga gcgcgagggc ggacaggacg
      901 gcgagcgttg ataatccgaa cagcaacgct atgcgggttc cgatggattg gccgatacgt
      961 atcttcgtcg tcgcgattgc catggtcgcc ctcctcgatc gtccaatgat catccagcac
     1021 gataattctt acgtatccgt tcgtagaatg caaccaaacg gtgctccgcg agattctata
     1081 aaataatgat attagaattc ctcgataaag cgtcacgcct tgccatggat cgagaagaac
     1141 ccaaccaacg cctgcagcct atgggcctgg tcggcgagct ccgtggaggt atcggcgact
     1201 agccgagctt gcccggctat ctcctcgctc gtagcgctga actcctccga gatggacgcg
     1261 ttctgctgga tgaccgagtc gagctgggtc atcgccaggt tgatctgctg cgccccggtg
     1321 tcctgctcgc ggcttgccac gctgatttcc tggaccaggt cggccgtctt ctggatatcg
     1381 ggggccatgt cctcgagcag cgcgcccgcc ttgtcggcta tctcgacgct acgctgcgaa
     1441 agcaccgata tctccccggc ggctagcttg gagcgctcgg ccagcttgcc cacctcgctc
     1501 gccacgaccg cgaaaccctt gccgtgctcg cccgcgcgag ccgcctcgat gctggcgttc
     1561 agggacagca tgttggtctg gctggcgatc tcctcgatga tggcgatgcg ctcggcgatc
     1621 tggcgcatcg cggccaccgt atccttgacg gccgtcaggc cttctctcgc gtcggtggcc
     1681 gccttggacg agatgcgctc ggtctggaac gagttgtcgg cgttctgctt gatgttggcg
     1741 ctcatctgct cgacgctggc ggacacctgc tcggcgctcg ccgcctgctc cgacgagccc
     1801 tgggacagct gttggctcga ttcggctatg ccggctatgc ccgcggccat ctgcttcgac
     1861 gcgtccgaca gcttggccga gccgtcgatc atcatggcgc tcgccccctg gacgttcgtg
     1921 atgacgtcgc gcagcttggc caccgtcgcg gccagggacg atacgagcat gcccgcctcg
     1981 tcatggccag cggccattct gccgtcgaag tcgtcgtcga ggcgaccggc cgccagatcc
     2041 ttgaccttgg ccgccgcgta gacgagcggc ttggcgatgc ggtcgccgag caggacggcg
     2101 acgacaatca tcgcgccgcc caggaccgcc gcgatgacga cgatgaggac gagggcgcgc
     2161 cggagcgacg cggccgcggc ttcgcgcttg gcggttatca cctggtcgat gtcgtccgtg
     2221 tagttgccgg tgccgatcac ccagttgaaa cccggcgcgt gcttactgta gccgcgcttc
     2281 ggagccgcct ccgtctcgcc tgcgcggggg aaccaatagt cggtgtaccc gccgcccttc
     2341 atggccgcgt cgatgatggc cctgacgagc tcgtaaccgt tgacgtcctt gagagccatg
     2401 cggttcgtac cctcggacgt gccgccgagg agcacgacgt tgtcgccctg gggcgtgtcc
     2461 gcccagaaat acccatcttc gccgtatcta agctcgcgga ggagcgtagc cgccagattc
     2521 cgagcctcgg cgtcgccaag cgagccttcg tcgcgaagcg ccgctaagcg ctcgagcatg
     2581 gagaccgccg tctcgatctc gctcttgatg agcaggtcga agtcgtcgcg aagcgtcgtc
     2641 tcgagcgcgg cgacctgctc gtcgaccgac gagcggagca ggttgatgaa tacgatcgac
     2701 agacagaccg cgacgacgag cgccgcagcc gcggcgagcg tcacgatctt ggttcggata
     2761 gatctcataa gcacggactc ctggtgatcg ataagctcat atatggtaag tgtatgtatg
     2821 aatcgatcga gcgcaaggaa ccgggcctgg cccgctccat cgcgagctta gcgatgtggt
     2881 atccgcatgt gcgctatccg agcgtgcgct atccgagcat gcgttcgatc gcgcggcggc
     2941 cgtactcgag ggcggtcatc atcgcggcgg cggatgcttc ggggtcgccc gagcctatgg
     3001 ccttcgcgat ggcgagctgt tgggcggccg tggcctcgag agcgcccggc agcgagtaga
     3061 agcgccgggt gtagaacagg tgtagctcgc ggctggtgtt ctgcatcgac ctgaacagta
     3121 cgttgcccgc gcccttgacg atgcggtcgt ggaaacggaa atccaactcc gccgcctccg
     3181 ccgggtcgcg ggtcgcggag agcgctgcgg ccatgttggc gaggtcggcg gagcaggcca
     3241 tcgggtccct cgtggcggcg aggcgggccg cggtcgtcat cagcatcatg cggtactcga
     3301 gcaggtcgag gagcagggcc gggtcgaggt catcggcgcg gcgcatcacg tggagtatca
     3361 ggtcgaacga ggcgtcgcgc aggtaatcgc ggacgtaggt gccgctctgc ggctcggtgg
     3421 acaggaggcc gagcgtctcg agcctgcgta tcgcctctcg gacgccgtta cggctggccg
     3481 agaagcgctc cgccagctcg cgctcggggg gcaggcggtc gcccggcttg agttcgccgc
     3541 cgagcacgag gcgctcgagc ctgaaggcta ccttatcggc tatcgtctgt ttcctatcct
     3601 cgaccgttac gtgcgccatg gcggctcctc gcgaaacagt agcatatgtg tcgagcgttc
     3661 gccagatgga tgaatatcct gtaaattatt ttaatataca aaacgacgat gcgcatgttg
     3721 aaataaaatc cgtcaaaaca gcaaaaacct ggtgacaaga ctatcttgac ttagtatact
     3781 gttgctacat ggagaatacg cgagcatgaa cgacgaaacc ggattggtgt cgaccaacct
     3841 gaccgagatg tccaagatcc tcgccgccac ggcggacgcc ggactcatag aggattttat
     3901 gtacagcctt tttacccagg ccgaggccga cgagatagcc aagcgctggg cgctggtcaa
     3961 gcgcatcgcc gagggcgtac cccagcgggc catcgccgcg gagctgggcc tgtcgctctg
     4021 caagatcacc cgcggatcga gggagctcaa gaaggagaat tcttcgttcc ggcgcatgct
     4081 cgagctcggc gggtacgaga tcgtggagaa gaagcggaaa gcctagtcct ggagccgacg
     4141 aggcgggacc atgaaggccg ctcggctatg gcccgggcgg ccttcatcgt ctcggggccc
     4201 gacgcgggtc ccatcgcggg gccttaggcg aggctggcgc tctcgatgag cacgtccgcc
     4261 ttgggcacgt tctggtgcgg gaagcggttg cccgtctcga ccttggctat ggcgtcgacc
     4321 acgtccatgc cgctcgtcac cttgccgaag gcgcagtagc cccatccggc ctggtccttg
     4381 gacctgaagt tgagcggctt gttgtcgacc aggttgatga agaactggca ggtggcgcta
     4441 tgcgggtccg aggtacgggc catcgcgatg gtgccgcgct cgttgcccac gccgttgtcg
     4501 gcctcgttct tgatcggcgc gttcgtatcc ttctccttga ggccgggcgt catgccgccg
     4561 ccctgcacca tgaagccggg gatgacccga tggaacacgg tgccgtcgta gaagccggcc
     4621 ttgacgtact cggtgaagtt cttgaccgtg acggggctct tgtcctcgaa gagctcgata
     4681 tcgaacgagc cgaggctcgt cttgaagttg actatcgggt tggccatgtc ggcctccttt
     4741 cgcgtagggt cagagcctaa cccgatacgg aggcgatagg caaggtcggc cggacctcat
     4801 ggtgacgacc gcggccgttt ggggtactat ctatccatag tggaggacgg cgatgaccaa
     4861 ggtactatct ctcggcggct ctatcgtggc tcccgacgtt ccggacgcgg ccttcgtcaa
     4921 ggcgttcgcg ctgacggcca ggcgctggct ggacgccgac gcggcgcgca ggctgatcgt
     4981 cgtggtcggc ggcggcggcc cggcccgggc gtggcagaag gcctaccgcg acgccgcgga
     5041 cgcgccggac gccgacgccc aggactggat aggcatcatg gccacgagat tgaatgccca
     5101 gctggtgcgc agcctattcc tggacctctg tccccatgag gtggtgaccg acccgacgag
     5161 cgtcgccgtg ttcggcggca gggtactcgt ggcggccggc tggaaacctg gcttctccac
     5221 ggacttcgat gcggtggtgc tggccgagcg cttccaggcc gacgtggtgg tcaacctgtc
     5281 gaacatcgcc aaggtctaca cggccgaccc gaggaccgac ccgaacgcca aggcgatcga
     5341 cgacatctcc tggaaggatt tccgcgcgat ggtcggcgac gagtggacgc cgggcaagaa
     5401 cgtgcccttc gacccggtcg cctcgaggcg ggccgaacag ctcggcctgc gcgtggtttg
     5461 cgccgccggc aaggacctgg ccaacctgga ggccattctg gatggcaagc ccttcgtcgg
     5521 cacgacgata ggcgcctgac gtcgggcggc tagcgctcgc ggagccgggc cagcagggcc
     5581 tcgtagcggg cggcgtagcc gtccttgccg agcgacgagg cgaggccgct caatgcggcc
     5641 aggcagcgct ccgcgtcgtc ggcgcggccg agcgacagcc agagcccgaa ggcccgacgg
     5701 tagtaatcga aggccgccgc gccgttcccc gacttgagct ggagcctggc gagcgcctcc
     5761 aggtcggccc cgatgcccgg cgagttctcc gccctcttgt ccgcctcgag ggccttggcg
     5821 gcccaggcga gcgcgcccgg caggtcgccg cgagcgttgg ctatcgaggc gagcgcgtag
     5881 cggttcgagc cgagctccga ccagcgccgc gccttctcgt tggcggcggc cgagcgcaac
     5941 acgtaggcct cggctgccgc atagtcggcg cgggccatcg cgacgacgcc gcggttgtgc
     6001 gcgaccacgg ccgctatcgc ctcgtctccg gcggccagcg cctcggcctc ggcgaagagc
     6061 gcgtcggccg cgtcccggtc gccgctcgcg tagcgcagct ccccgaggtt cgacaggcac
     6121 agcgcgacga gggccggctc tccgagggcg cgcgcgtcct cgagcgcgtc gccgaactcg
     6181 cgggccgcgt cgtcgaggcc accgatggcc aggtaggcgc ggccgagcga cccgcgggac
     6241 ttgaccgctc ccgcgacatc gtcgaccgac aggctggcgt cgagcgcctc tccgtagtac
     6301 tgcagcgccg acgagtactg gcccgaggcc aggaagccgt cggccagggc ggcgtactcg
     6361 gccgcctcgt tcttgcgctc gacgacggcc gtctgccgct tgggcgcgct cgagcaggag
     6421 ccaagccagc ctgaggccag ggtcgcggcg gctacgagcg ccagggcccg cgaccgtcgc
     6481 ccgaagtacc ggcgcgccac cgggcgcggc gcgccgtagg gcgcgcctgg aagatggtat
     6541 atcgtcgcct gaagcgcccg tctcgtcgcg cccgatacgc ccttcgtcgt cattcgaagc
     6601 tcccctcgcg catcgcgctg tacagcgatt cctgctcgac ccgctcggtg accccgccgc
     6661 ggatcagcgg gttgttcttg acgccctcga gcacgtcctg ggcctgcttg atcgcggcct
     6721 tggtctcgtt gaggaggccg gcgatcttgg gcatctcgcc gttcagcccg gtgatgatgc
     6781 cctggaggct acgcaccgcc t
//
LOCUS       DAOPHV010000065         6763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_14500_length_6763_cov_3.812463, whole genome shotgun sequence.
ACCESSION   DAOPHV010000065 DAOPHV010000000
VERSION     DAOPHV010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14500_length_6763_cov_3.812463"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="PLQ29_02875"
     CDS             <1..1401
                     /locus_tag="PLQ29_02875"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HPG85618.1"
                     /translation="PAEVEEAVAAAAAAYPAWSSTPVTKRVQVLFRLKALMDARLDEL
                     AELTAREHGKVWDEAMGDVLKAIEVVEYACGAPSLMQAPALMDCSPGYDTAQYRESLG
                     VFAGIVPWNFPAMIPMGWMAPLCVATGNTMVIKAASYTPQTAMRIMELWAEAGLPPGV
                     LNLVTCSRAEAELLLRHPAIVGVSFVGSTSVGRHIYETAAAAGKRVQCLTEAKNHALV
                     LSDAAVERTAAGIVNSACGCAGERCMALPVVVAEEAIADELVASLRRRMEGLRIGPAY
                     DKASRLGPLVNAEHRRSVEAWIRKGVDEGAELVLDGRGVSVPGYEGGFYLGPTLFDRV
                     APGMSIGEREIFGPVLCVKRVRDFDEGLAVMNAGEFANGSVIYTRSGYHAREFARRTH
                     GGMVGVNVGIPVPLGLFGFTGRKASFFGDLHAMGPDGVRFYTDLKSVTSHWFSEEEAR
                     EGSVLNSWDGMIATAR"
     gene            complement(1442..2830)
                     /locus_tag="PLQ29_02880"
     CDS             complement(1442..2830)
                     /locus_tag="PLQ29_02880"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPG85619.1"
                     /translation="MKRLRLKTYLIVLPIIAATVVVSGFVAYQESRSSLTRLASRHLA
                     YKAEQLRDFINSEWSVVESLGLGDDHAYRKAQEASFRSYAYALLRSDSEAIAVYGDDG
                     RVRFIVSGSGSEDEEAFPMPGTTAGWFSSSSRSGSRVGIAFPFEPFGWTVALSESADY
                     FYADERDLLRGFGWILSIALSAAVLLTAVYLRRVTGPLERLSDAVARIADTGDLARRV
                     PEEYDDEIGDLAARFNSTLAALEARTSQLQAAAEAERQAHATTEERERETLWLLARIA
                     DYHDEETGAHVQRIGELSAFMLRLLGQGDEEQQLIRAAAPLHDIGKIGVSESLLNKPD
                     RLTADEFEVMKTHAAIGHELLGTCHSRYLAEGAEIALTHHEKWDGSGYPRGLSGEGIP
                     LSGRVVAIMDVYDALVSDRSYKKVWAAADAREYIAGQRGAHFDPRLVDLFLERFDEFA
                     ALRDALADGPSN"
     gene            2902..3921
                     /locus_tag="PLQ29_02885"
     CDS             2902..3921
                     /locus_tag="PLQ29_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85620.1"
                     /translation="MTSHRRGDYSLFVLNAVIVTLAVAIMLVAAARYANDARSETLGS
                     TSYFTTEWRLLRQLKESTDRALSAKDAEIEELRERYQSLQRSGASASLLRSIDEAIRE
                     AEEGRAVILSARVSAYEGRDGAGGVVAGNGGSSSSRAVAAALATVAAVESAKPSPADA
                     EMAPSAGRAPVGQPRPVASPSELLLNWIRDLESSLADEAARADSAEREILALRSALAE
                     ARAFEAFNHGLASDGAAETGASGAPGEMVPGSSGTLASDLMVDVAAELRRLSDAEPLI
                     GMSDLRTRALLRAIVRDPAIRAEYPELFDSLDTYLERVEADAHQRGRAAAYEDILERL
                     GATGR"
     gene            complement(4141..4578)
                     /locus_tag="PLQ29_02890"
     CDS             complement(4141..4578)
                     /locus_tag="PLQ29_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85621.1"
                     /translation="MKRTPPKHTYPLSPIAPIALMALAISSLSVACDEFSFVSLLSLP
                     DQATPDELSLTVAQDSVPAGSSVALEVSGGVPPYTFKPLSGSDLYQPTSNRSLGTISD
                     SVFTAGQAIGKILITVEDAVGYLRFRLLSYSRPLRFLCNPLSE"
     gene            complement(4583..5170)
                     /locus_tag="PLQ29_02895"
     CDS             complement(4583..5170)
                     /locus_tag="PLQ29_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85622.1"
                     /translation="MNGSAAAPEAAPPIAIGGLFSSELLRLEDTLRTTDTRRLEALAR
                     AGVTSFVGGFADYARLGATAALDVAALRLDPAWSLSVGGRASATRAFMNLGVTGGTMY
                     LATIGPDLRAGIGARQAQRLSVGLSGGVAILALALDDGTTLARAEPFVDAGVQAGVPL
                     GRDFFLGLDARFVVVFDPEMLIMGTSPTITLFKEF"
     gene            complement(5167..5658)
                     /locus_tag="PLQ29_02900"
     CDS             complement(5167..5658)
                     /locus_tag="PLQ29_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85623.1"
                     /translation="MAHLNARRGLMVCMTLAFALSVPAFGADAAKAPPRTPISIGFSS
                     PAADSEFEDVFYLSIAIILADRGYPSVKAAPADEPVLLVGYEVSGRSAMVRLELAEGP
                     GRRAASAEGMVRLTLELDADLELLVDDLLGRAVPQAPSPALAGSDEATKTSSGVEAAK
                     RLL"
     gene            5876..>6763
                     /locus_tag="PLQ29_02905"
     CDS             5876..>6763
                     /locus_tag="PLQ29_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85624.1"
                     /translation="MGAGQAFGGVMGQAASTAADGTHIAATGLMAKTGIGGVMARSVW
                     SGMQTLTTSTLSSGINAVYWGENGLDFNDDAFASGIKGGFVSAATGMASSITSGSLGQ
                     WNLQDGNGIGLSSKIFTTEDIGRFNEFAGGLAGQGLNYALTGETTFNLANFDMFGMQG
                     VNGNRVSSGLLELHLGKNGMSMNLGTGGMDASIGAIAASLSGLSETAKIGRSKIAAIF
                     GQQEGLSTLNAVNLLGYTNDGFDEDLGRSIWSGRIRARYGDTGSDWGRYDSEAPDSIL
                     LSDRLLGGGTEEAAKLATVM"
BASE COUNT         1151 a         2095 c         2398 g         1119 t
ORIGIN      
        1 ccagcggagg tggaggaggc cgtcgccgcc gccgcggccg cctatcccgc ctggtcgtcg
       61 accccggtga cgaagcgcgt ccaggtcctg ttcaggctca aggccctgat ggacgcccgc
      121 ctggacgagc tcgccgagct gacggcccgc gagcacggca aggtctggga cgaggcgatg
      181 ggcgacgtgc tcaaggcgat cgaggtggtc gagtacgcgt gcggcgcgcc gtcgctgatg
      241 caggcgcccg cgctgatgga ctgttcgccc ggctacgaca ccgcccagta ccgcgagagc
      301 ctcggcgtgt tcgccggcat cgtgccgtgg aatttcccgg cgatgatacc gatgggctgg
      361 atggcgcccc tctgcgtggc cacgggcaat acgatggtca tcaaggcggc cagctacacg
      421 ccccagacgg cgatgcgtat catggagctc tgggcagagg ccggcttgcc tccgggagtc
      481 ctcaatctgg tcacctgctc gagggccgag gcggagctac tgttacggca cccggcgata
      541 gtcggcgtca gcttcgtcgg ctcgacgagt gtcgggcgcc atatttatga gacggccgcc
      601 gctgcgggca aacgggtgca gtgcctgacc gaggccaaga accacgccct ggtcctatcc
      661 gacgcggccg tcgagcggac ggccgccggc atcgtcaact cggcctgcgg ctgcgccggc
      721 gagcgttgca tggcgcttcc ggtcgtcgtg gccgaggagg cgatagccga cgagctcgtg
      781 gcatcattgc ggcgccggat ggaagggcta cggatcgggc cggcctacga caaggcctcg
      841 aggctcgggc ccctcgtcaa cgcggagcac cgacgctcgg tcgaggcctg gatccggaag
      901 ggcgtcgatg agggcgccga gctggtcctc gacggccgcg gcgtctccgt gcctggttac
      961 gagggcggtt tctacctggg cccgactctc ttcgaccgtg tggcccctgg catgtcgatt
     1021 ggcgagcggg agattttcgg gccggtcctc tgcgtcaagc gcgtgcggga cttcgacgag
     1081 ggcctcgccg tgatgaacgc cggcgaattc gcgaacggct cggtgatcta tactcgctcg
     1141 ggctaccacg cccgggagtt cgcgcgacgc acgcatggcg gcatggtcgg cgtcaacgtc
     1201 ggtattccgg tgccgctcgg cctgttcggg ttcaccggcc gcaaggcgtc gttcttcggc
     1261 gacctgcacg ccatggggcc cgacggcgtg cgcttctata ccgacctcaa gagcgtcacc
     1321 tcccactggt tctccgagga ggaggccaga gagggctccg tgctcaactc ctgggatggc
     1381 atgatcgcga cggccaggtg acggggcggc tcctggaccg gggctggagc cgtagccccg
     1441 gctaattgga ggggccgtcc gcgagggcgt ccctgagcgc cgcgaactcg tcgaagcgct
     1501 cgagaaacag gtcgacgagg cgtgggtcga agtgggcgcc gcgctggccg gcgatatatt
     1561 cgcgcgcgtc cgcggcggcc cacactttct tgtaggaacg gtccgatacg agcgcgtcgt
     1621 agacgtccat gatggcgacg acgcggcccg agagggggat gccttctccg gacaagcctc
     1681 gcgggtatcc cgagccgtcc catttctcgt ggtgcgtcaa cgctatctcg gcgccctcgg
     1741 cgaggtagcg gctgtggcag gtgccaagga gctcgtggcc tatcgccgcg tgcgtcttca
     1801 tcacctcgaa ttcatccgcc gtcagccggt ccggcttgtt gagcaggctc tcgctgacgc
     1861 ctatcttgcc gatgtcgtgg agcggggcgg cggcgcgtat gagctgctgc tcctcgtcgc
     1921 cctggccgag caggcgaagc atgaaagccg acagctcgcc gatgcgctgg acgtgggcgc
     1981 cggtctcctc gtcgtggtaa tcggcgatac gggcgagaag ccagagtgtc tcgcgctcgc
     2041 gctcctcggt ggtggcgtgg gcctgacgct ccgcttcggc cgcggcctgg agctgagatg
     2101 tccgggcctc gagcgcggcg agggtggagt tgaagcgggc ggccaggtcg cctatctcgt
     2161 cgtcgtactc ctccggtacg cgccgcgcca ggtctccggt gtcggctatc cgggcgacgg
     2221 cgtcgctcag gcgctcgagc ggccctgtga cccggcgtaa gtagaccgcg gtcaggagca
     2281 cggccgcgct gagcgcgatc gaaaggatcc agccgaagcc gcggaggaga tcccgctcgt
     2341 cggcgtagaa atagtcggcg ctctcgctga gcgcgacggt ccagccgaac ggctcgaacg
     2401 ggaaggctat gccgacgcgg gaccccgaac gggatgacga ggagaaccat cccgccgtcg
     2461 ttccgggcat agggaaggcc tcctcgtcct cggaaccgga gccgcttaca atgaagcgca
     2521 cgcggccatc gtcaccgtag acggctatcg cttcgctatc gctacgcaag agcgcgtacg
     2581 cgtaggagcg gaacgaggct tcttgtgcct tgcggtacgc gtggtcgtcg ccgagcccga
     2641 gcgactccac caccgaccat tcgctgttga tgaagtcgcg cagttgctcc gccttgtagg
     2701 ccaggtggcg gctggcgagg cgggttagac tggatcggct ctcctgatag gcgacgaagc
     2761 cgctgacgac gacggtggcg gcgatgatag gcagtacgat gaggtaggtc ttgagtcgta
     2821 ggcgcttcat gtcgttagca ttctatagta ttatcataat tctgaccaat tggtatcatc
     2881 acgaggggag cgacgcgtat tttgactagc cataggcggg gcgattattc gttgttcgta
     2941 ctcaacgccg tcatcgtcac gctagcggta gccatcatgt tggtcgccgc ggcccgttac
     3001 gccaacgacg cgcgttctga aaccctcggc tcgacgagct attttacaac cgagtggcgg
     3061 ctgcttcggc agctcaagga gtcgactgac agggccttgt ccgccaagga tgccgagatc
     3121 gaggagctgc gcgagcgcta ccagtccctc cagcgctccg gcgcgtcggc cagcctgctc
     3181 cggtccatcg acgaggcgat acgtgaggcg gaggaaggcc gcgcggtcat cctgtcggcg
     3241 cgcgtcagcg cctatgaagg ccgcgacgga gcgggcggcg tcgtagcagg caatggaggg
     3301 tcctcgtcta gccgggccgt cgcggcggcc ctggcaaccg tcgccgcggt cgagtcggcc
     3361 aagccttcgc cggccgacgc cgagatggcg ccgagtgccg gccgcgcccc cgtaggccaa
     3421 cctcgtccgg tagcgagccc ctccgaactg ctcctgaact ggattcggga cctggagtcg
     3481 tcgctggcgg acgaggcggc gcgggcggat agcgccgagc gcgagatact ggccttgcgt
     3541 tcggccctgg ccgaggctcg ggcgttcgaa gccttcaatc acggcttagc ttccgacggc
     3601 gctgctgaaa ccggcgcgtc tggcgctccg ggagagatgg ttcccggttc gtccggaacg
     3661 ctagcgtccg acctgatggt tgacgtggca gccgagctta ggcgcctgtc cgacgccgag
     3721 ccgcttatcg gcatgtccga cctgcgcacg cgcgcgcttc tgcgagccat cgtccgcgac
     3781 ccggccatcc gagccgagta ccccgagctg ttcgactcgc tcgatacgta cctggaacgc
     3841 gtcgaggcgg atgcccacca gcgcggccgc gccgccgcct acgaggatat actggagcgg
     3901 cttggggcta ctggaagata attgttacgg gcatggactc gtatgtgccc gagagagcga
     3961 agagcctgaa ccctaacgtc gccgccgctg gttgttggtt gaaggtggcg gacacgctat
     4021 ccgcatcgta cgaagctagg aacgagtatt cggagccatc gatagaccgc tcgagccgat
     4081 aaccattgat catggcgacg tcaggatagg accagtaaag ttgtaccgtc gtccctcctc
     4141 ctattcgctt agaggattgc ataaaaaacg gagtgggcgg gagtacgaca atagacgaaa
     4201 acgtagatac cccactgcgt cctccacggt gatcagtatt ttcccgatag cctggccggc
     4261 ggtaaagacg ctgtccgaga tggtgccgag gctacggtta ctggtcggct ggtacaggtc
     4321 ggagccggat agcggcttga aggtatacgg aggtacgccg ccgctcacct cgagggcgac
     4381 tgagctgccg gcgggcacgc tgtcctgggc gaccgtcagc gacagctcgt caggcgtggc
     4441 ctggtcgggc agggacagga gcgacacgaa cgagaattcg tcacaggcga cggacagcga
     4501 ggagattgcg agagccatga gcgcgatcgg cgctatcggg gacagaggat aggtatgttt
     4561 cggaggcgtg cgcttcatac ggctagaatt ccttgaacag cgtgatggtg gggctggtgc
     4621 ccatgatgag catctcgggg tcgaaaacga cgacgaatcg ggcgtctagg cccaggaaga
     4681 agtcgcgccc gagcgggacg ccggcctgga cgcccgcgtc gacgaagggc tccgcccgcg
     4741 ccagggtcgt gccgtcgtcg agggccagtg ccaggatcgc gacgccgccg gacagcccga
     4801 cggacagacg ctgggcctgg cgcgcgccga tcccggcgcg taggtcaggg cctatcgtcg
     4861 ccaggtacat cgtgccgccc gtcacgccaa ggttcatgaa cgccctggtg gcgctcgcgc
     4921 ggccgccgac cgacaggctc caggccgggt cgagcctgag ggccgccacg tcgagggcgg
     4981 cggtggcccc gaggcgggcg tagtcggcga agcccccgac gaaggacgtg acgccggcgc
     5041 gcgcgagcgc ttcgaggcgc cgtgtatcgg tcgttcgcag ggtgtcctcc aggcgcagga
     5101 gctcggacga gaagagtccg ccgatggcga tcggcggcgc cgcttccgga gcggccgcgg
     5161 agccgttcac agaagccgct tcgccgcctc gacgccggag ctagtcttcg tagcctcgtc
     5221 cgaaccggcc agggccggcg atggcgcttg cgggacggct cgcccgagta gatcgtcgac
     5281 gagaagctcc aggtcggcgt cgagctcgag cgtcaggcgg accatgccct ccgcggaggc
     5341 cgctcgtcga cccggccctt cggccagctc gagcctgacc atggccgagc ggccggagac
     5401 ctcgtacccg acgagcaaca cgggctcgtc ggccggcgcc gccttgacgc tcggatagcc
     5461 cctgtccgcc aggatgatcg cgatcgagag gtagaacacg tcctcgaatt ccgagtccgc
     5521 ggcgggcgac gagaagccta tcgagattgg ggtgcgcggc ggagccttcg ccgcgtcggc
     5581 cccgaaggcc ggcacggaaa gggcgaaggc gagcgtcatg cataccatga gcccccgccg
     5641 cgcgttcagg tgtgccatac ggctcaggat accctgaatg gagagccctt gcataccgcc
     5701 gaggcgccta tacggcgcta cgtcgagcct tcgttgacgg cgtcgggagc ccgtcgtact
     5761 attcgggaat gaaacagatc aggatcctgc cgcgtactgg tcggtcaagg aaggcaaggg
     5821 ttggttcgag gccgggctcg atttcgggaa gaaggcgctc agctctgccg tcaacgtggg
     5881 cgcgggacag gcgttcggcg gcgtgatggg gcaggccgcg tccacggccg ccgatggtac
     5941 gcacatcgcg gccactggac tcatggctaa gacgggaatc ggaggcgtga tggcgcgttc
     6001 cgtatggagc ggcatgcaga cgctcacgac gagcacgctg tcgagcggga tcaacgccgt
     6061 gtactggggc gagaacggcc tcgacttcaa cgacgacgcc ttcgcctctg gaatcaaggg
     6121 cggcttcgtg agcgccgcta cgggtatggc gagttctata acgtccggta gcctcgggca
     6181 atggaatctc caggacggca acggtattgg cctgagctcg aagatcttca cgaccgagga
     6241 tatcggacga ttcaacgaat tcgcgggcgg cctcgcgggg caggggctga actacgcgct
     6301 gaccggggag acgaccttca acctcgcgaa cttcgatatg ttcggaatgc aaggcgtgaa
     6361 cggcaaccgc gtttcgagcg ggctcctcga gctgcacctt ggcaagaacg gcatgtccat
     6421 gaacctgggc acgggcggca tggacgcgag cataggggcg atagcggcga gcctgtctgg
     6481 attgtcggag accgcgaaga ttgggagatc gaagatcgcc gcgatcttcg gacagcaaga
     6541 aggattgtcg acgttgaacg ccgtaaacct gctgggttac acgaacgacg ggttcgacga
     6601 ggacctggga cggagcatct ggtcggggcg gatcagggcg cgatacggcg acacgggaag
     6661 cgattgggga cgctacgatt ctgaagcacc cgacagcatc ctcttgtccg atcggctact
     6721 tggcggaggg accgaggaag ccgcgaaact ggctacggtg atg
//
LOCUS       DAOPHV010000066         6544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_15239_length_6544_cov_4.634767, whole genome shotgun sequence.
ACCESSION   DAOPHV010000066 DAOPHV010000000
VERSION     DAOPHV010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15239_length_6544_cov_4.634767"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2126)
                     /locus_tag="PLQ29_02910"
     CDS             complement(<1..2126)
                     /locus_tag="PLQ29_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPG85625.1"
                     /translation="MQKLFKSTLRHPFIVMSLALGITALAVLSVVSGFRMETNLDTYM
                     PRTHPAFVFSDEADERFMIKDSILIAVEHPYSVFNPATLQKLIDIEDDLLALEGLSGA
                     RVNSIHTGDNILATEDGLDVRRFYTDAPIDQEGADRIASAVRGNSMIAGRLVSHDERT
                     ALIIVELPESGFSRDLYDQIQQIADERTGPETLHIAGRPIVEGTLAALGPADMARMGP
                     LVLLVIAIVLLLVLRQVRRTIITMSVVVFSTIWAFGLMSALGVPMYSVSIMIPVMLIA
                     IGVAYAIHLYNQIDHFARETPGAGRRDIAENAITVISSPALFAALTTVAGFVSLVTSR
                     VYPVKYFGLFTAFGVFASFVLTMLLVPAGIMAFGNGDVKKAAGAHAGRIPEHGLGARF
                     ADALKARPGRVYVIVAVLVAASVFGISRVWINTSFLANFDSNSAIVKTDAFVNEHFGG
                     TSSLNVILEAADPDTFKNPEVLRLMDAMQAGALELENVGDTISLTTYLRRLHYSMNAA
                     DPAFDVIPDSSDLVAQYLLLYEMSGDPDNLWKVVDADFRSANVTIQMKSDDTRTIGRV
                     IEYVDAQAADFDRFGITIRYAGSGYKSLIFSDLILQGQMSSLGLSVIIVFILVAFMFR
                     SVLLGLVSTIPVLISIAVNFGVMGLLNVPLTTSTALISSIAVGIGVDYAIHLITHYRE
                     ALKALGDEERAARFAMSHTGRAVLLNA"
     gene            complement(2212..2805)
                     /locus_tag="PLQ29_02915"
     CDS             complement(2212..2805)
                     /locus_tag="PLQ29_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPG85626.1"
                     /translation="MQTSERQKEIIQAAFDLISEQGIQELTIKKIAQVVGVTEPAIYR
                     HFTSKVDILSAVIDEMVAQRNAAFLRCGIGSGTARDMIRSFFAVQASFFESNPSLSIM
                     LFPEDLFRNNADLLGRATSMMDETLARFRELLLAGIDEGSLKPGVDCEAGALMLVGGF
                     RLLVASWRLDAGTGTGAGGLETDTARFMEGVLSLISR"
     gene            2963..4183
                     /locus_tag="PLQ29_02920"
     CDS             2963..4183
                     /locus_tag="PLQ29_02920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG85627.1"
                     /translation="MPMQRTQRTEAPDRLVRTPYLWSLYLLLGLFSFMLSMIGPMVPY
                     LRDEFSLSYGLAGLHQSAFALGMVIMGFLGSSVIRRLGTALSLWGGMADMLAGLLVMI
                     LAHGPAMTLTGVFVMSLGGTVALAAIQTSLANGPAAHRGKLIMEANVMASALTMLVPL
                     VLLAGSRMTMGWRIVFPAMLVSVAATASFGLPATMRHLKSRDERADAGGGRLGGAFWR
                     MWLIVFFGVSVEWAVSFWCMTYLLGLPGNSRELAAAGTVLLGLSAVAGRFFSSRVSHR
                     IPEKRLLYLMMLVVAAGFPLYWLRASTATAFVGLALCGIGSSNFYPLGLSLAVGHAPG
                     NAAKAGSLIPVASGSAIGLAPFLLGRLADLTDMSAALWYIPIGVLLILAIALVDRAFE
                     RRTGLGAADRQRTE"
     gene            4223..6202
                     /locus_tag="PLQ29_02925"
     CDS             4223..6202
                     /locus_tag="PLQ29_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HPG85628.1"
                     /translation="MIAILATVGALPFVVFSMAFLSVIGDRLAEYSETVSTAALQAVI
                     ATARERAIEGPRRDLPALLLQAADEADGSRLTELLRDTLAPRPEYLALIVLDAAGRVV
                     ASYPDTGLSPGYVYRAKAARTASGVTAFSPPFQSDTFGRPVIEAAYAGERRSVVAIID
                     LEWLSAKLYLAMASPQDRVGIVWLDGRYLASSDPSRMRSGERVDARLLGGKPTAYADE
                     RGEYTVLSAPIHGSDWRGVYYRSNLPIRESTATFGLRITMLGLVSFALAGLIAYLVWH
                     RLLTPLSTLTRKIAAIAEGRYGERLELDVPDELMEMARAINSMAESIERRGEELERSE
                     RRYRMTFERSGAPVLLLSADDASVRDANPAALEYYGYAPEEIRRLGIADLDASGRKVQ
                     AYKGADRLSSRHRLSSGEIRDVILYVSPLELGDEELLHALVFDVTDQRIAEERSVAAL
                     EEKTLLLREVYHRVKNNLQVISSLLSMQAAVTADRSVSTALQGAQDRVFAMSLAHEIV
                     YQMPDISSVDAGDYASRLVAYLADEYKVPRDSVLAAYEPMPLSLERAVPFGLLLGEIV
                     SNALKYGIPPGTDGRVRVSLSLDEAGRDRMVCLVVEDDGPGLPPEVADKGSLGISLIA
                     GLADQLRGTVAWERPSGGGTRVTVRFDSSADGKAR"
     gene            6199..>6544
                     /locus_tag="PLQ29_02930"
     CDS             6199..>6544
                     /locus_tag="PLQ29_02930"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85629.1"
                     /translation="MTAGTRLAAMAMAVAVSAAACDQRPIVTLRYAHMSPLDSVAGMQ
                     AEYFARRARELSEGRLVVEVFPASVLGDTAEQLAMARDGVIDIHHTTAGALASLVEGF
                     TVLDAPYRVSDPE"
BASE COUNT         1134 a         2117 c         2138 g         1155 t
ORIGIN      
        1 gcgttgagta gcaccgccct gccggtatgg ctcatcgcga agcgggcggc acgttcctcg
       61 tcgccgaggg ccttcagcgc ctcgcggtaa tgggttatca ggtggatggc gtagtcgacg
      121 cctatgccca ccgcgatgct ggatatgagg gccgtggagg tggtcagggg cacgttgagg
      181 agccccatga cgccgaagtt gaccgctatc gatatgagca ccggaatcgt ggacacgagg
      241 cccaggagca cgctgcggaa catgaaggcc acgagtatga agacgatgat cacggagagg
      301 ccgaggctgc tcatctgccc ctgcagtatg aggtccgaga agatcaggct cttgtaaccg
      361 gatcccgcgt agcggatggt gatgccgaac cggtcaaagt cagccgcctg ggcatcgacg
      421 tactcgatga cgcgcccgat ggtgcgggta tcgtcgctct tcatttggat ggtgacgttg
      481 gcgctcctga aatccgcgtc caccaccttc cagaggttgt ccggatctcc cgacatttca
      541 tacaggagca ggtactgggc caccagatcg cttgagtcgg ggatgacatc gaaggcgggg
      601 tcagccgcgt tcatggaata gtgcaggcgc ctgaggtagg tggtcagcga tatggtgtcg
      661 ccgacgtttt caagctccaa agccccggct tgcattgcgt ccataaggcg aagcacctcc
      721 ggattcttga aggtatccgg atcggccgct tccaggatca cgttaaggct ggacgtgccg
      781 ccgaaatgct cgttgacgaa ggcgtcggtc ttgacgatgg cgctgttcga gtcgaagttg
      841 gccaggaaac tggtattgat ccatacccgg gagattccga atacggaagc cgcgaccagg
      901 accgcgacga tcacgtagac gcgcccgggc ctggccttga gggcatcggc gaaccgtgcg
      961 ccaagtccgt gttcgggtat gcgcccggcg tgggcgccgg cagctttttt gacgtcgccg
     1021 ttgccgaagg ccatgatgcc cgccggcacg aggagcatcg tcaacacgaa tgacgcgaag
     1081 acgccgaacg cggtgaagag cccgaagtac ttgaccgggt agacccgcga ggtgacgagc
     1141 gacacgaacc cggcgaccgt cgtcagcgcc gcgaacaggg ccgggctact tatgaccgtt
     1201 atggcgtttt ccgcgatgtc gcgccggccc gctccaggcg tttccctggc gaagtggtcg
     1261 atctggttat acaggtggat ggcataggcg acgccgatgg cgatgagcat caccggtatc
     1321 atgatgctga ccgagtacat ggggacgccg agcgcgctca taagcccgaa agcccagatc
     1381 gtactgaaca cgacgaccga catcgtgatg atggtacgcc gtacttgcct gaggaccagg
     1441 aggaggacga tagctatgac gagcagtacc aggggtccca tcctggccat gtccgcgggg
     1501 ccgagcgcgg cgagcgtgcc ttcgactatc ggccggccgg ctatgtggag ggtttccggt
     1561 cccgttcgct cgtccgcaat ctgttggatc tgatcataca gatcccgcga gaaaccggat
     1621 tcaggcagtt caacgattat gagggccgtg cgctcgtcat gggagacgag acggccggct
     1681 atcatgctgt tgccccggac ggcgctggct atgcggtcgg cgccttcctg gtctatagga
     1741 gcgtcggtat agaatctgcg cacgtccagt ccgtcttccg tagccaggat gttgtcgccc
     1801 gtatggattg agttgacgcg ggctccggac agtccttcca gcgccaggag gtcgtcctcg
     1861 atgtcgatca gtttctggag cgtcgccgga ttgaacaccg aatagggatg ctccacggcg
     1921 atgaggatcg agtcctttat catgaagcgc tcgtcggctt cgtcgctgaa cacgaacgcc
     1981 ggatgcgtgc ggggcatgta ggtatccagg ttggtttcca tcctgaaccc tgaaaccacg
     2041 gaaagcaccg cgagcgcggt gatgcccaga gctagcgaca tcacgatgaa cggatggcgc
     2101 agggtggact tgaatagctt ctgcattggc aacccctttg gtgtggcaac atgagttagt
     2161 tatcgttcac aaaccttagc agataaaaaa atgatcgcgg caaggacgat gtcatctgct
     2221 gatcagggag agcacccctt ccatgaagcg agctgtatcc gtctcaagac cgccggcccc
     2281 ggttcccgtt ccggcgtcca gccgccagga cgccaccagc aggcggaagc cgccgacgag
     2341 cataagcgcg ccggcctcgc agtcgacgcc gggtttaagc gagccttcgt cgatccccgc
     2401 caacagcagt tcgcggaacc gggcgagggt ctcgtccatc atcgatgtcg ccctgcccag
     2461 caggtcggcg ttgttgcgga acagatcctc ggggaacagc atgatggaca acgatggatt
     2521 gctctcgaaa aagctggcct gtacggcgaa gaacgagcgg atcatatcgc gagccgtacc
     2581 tgaaccgatc ccgcaccgca ggaaggcggc gtttcgctgc gcgaccattt cgtctatgac
     2641 cgcggacagg atatcgacct tcgacgtgaa atggcggtag atggcgggtt cggtgactcc
     2701 gacgacctgg gcgatctttt ttatggtgag ttcctggatg ccctgttcag agatcaggtc
     2761 gaacgccgcc tgaatgatct ctttctgccg ttcgctagtc tgcaccgatt ctccccacca
     2821 agttagtgat cactaaccta cagtacaacg agaaggaacg tcaagacgtc taccaaaaaa
     2881 acgaccccgg agggcacgca agctcgaacc tggatcggag cccctcgatg acagcgcggt
     2941 cgaaataggc tataccttat gcatgcccat gcaacgaaca cagcgaacgg aagcgcccga
     3001 ccgactcgtc cgcacgccgt atctatggtc gctctacctg ctcctcggcc tgttctcgtt
     3061 catgctgtcg atgatagggc cgatggtgcc gtacctgcgg gacgagttct cgctcagcta
     3121 cggcctggcc ggcctccatc agagcgcctt cgcgctcggc atggtcatca tgggcttcct
     3181 cggcagttcg gtgatccggc gcctcggcac ggcgctgagc ctctggggcg gcatggccga
     3241 catgctggcc ggcctcctcg tgatgatcct ggcgcacggc ccggctatga ccctgacagg
     3301 cgtcttcgtg atgagcctcg gcggcaccgt ggcgctcgcc gccatccaga cctcgctggc
     3361 caacggccca gcggcgcatc gcggcaagct gatcatggag gccaacgtga tggccagcgc
     3421 gcttacgatg ctggtgccgc tcgtgctgct cgccggctcg cgcatgacga tgggctggag
     3481 gatcgtgttc ccggccatgc tcgtctcggt agcggccacg gcttccttcg gcctaccggc
     3541 gacgatgcgc cacctcaagt cgcgcgacga gcgcgcggac gccggaggcg gcaggctcgg
     3601 cggcgcgttc tggcgcatgt ggctgatcgt gttcttcggc gtatcggtcg aatgggccgt
     3661 cagcttctgg tgcatgacct atctgctcgg cctgcctggc aactcgcggg aactcgccgc
     3721 ggcgggcacg gtcctgctcg gcctgtcggc tgtcgcgggg cgcttcttct cgagccgcgt
     3781 cagccacagg atacccgaga aacggctcct ctacctgatg atgctcgtcg tcgcggcggg
     3841 cttcccgctg tactggttgc gcgcgagtac ggcgacggcc ttcgtcgggc tggccctctg
     3901 cggtatcggc tcgtcgaatt tctatccgct cgggctgtcg ctcgcggtcg ggcacgcccc
     3961 aggcaacgcc gccaaggccg gatcgctgat accggtcgcc tcgggctcgg ccatcggcct
     4021 ggcgccgttc ctgctcggcc gcctcgccga ccttaccgac atgagcgcgg ccctctggta
     4081 catacccatc ggcgtgctcc tgatcctcgc catcgccctg gtcgaccgag ccttcgagcg
     4141 gaggaccggc ctcggggcgg ccgatagaca gcgcacagaa taggcgtagg ataccatgcc
     4201 aggcggtagc gtacggcggc gtatgatagc catcctcgcg acggtaggcg ccttgccctt
     4261 cgtcgtcttc tccatggcct tcctgtcggt gatcggagac cggctggccg agtactccga
     4321 gaccgtgtcg acggcggcgc tccaagccgt gatcgccacg gccagggagc gcgccatcga
     4381 gggcccgcgc cgagacctgc cggcgctgtt gctgcaggcg gcggacgaag ccgacggctc
     4441 tcggctgacc gagctgctcc gcgacaccct cgccccccgg cccgaatacc tggccctgat
     4501 cgtactcgat gccgccggcc gcgtcgtcgc ctcctacccc gacaccggac tatctcccgg
     4561 gtacgtctac cgcgccaagg cggccaggac ggcctcgggc gtcacggcct tctcgccgcc
     4621 gttccagtcg gacaccttcg gccgcccggt catcgaggcg gcctacgccg gcgagagacg
     4681 gtccgtggtc gccatcatcg acctcgagtg gctatcggcc aagctctacc tggcgatggc
     4741 ctccccgcag gatcgggtcg gcatcgtctg gctcgacggc cgctacctgg cctcgagcga
     4801 cccgtccagg atgcgctcgg gcgaacgggt cgacgcgcgg ctactcggcg gtaagccgac
     4861 agcctacgcc gacgagcggg gcgaatacac ggtgctgagc gcgcccatcc acggctcgga
     4921 ctggcgcggc gtctattaca gatccaacct gccgatacgg gagtctaccg cgaccttcgg
     4981 gctacgtata accatgctcg gcctggtctc cttcgccctg gccgggctga tagcctacct
     5041 cgtgtggcat cggctcctga caccgctctc gacactgacg cgcaagatcg cggcgatcgc
     5101 cgagggccgc tacggcgagc ggctggagct cgacgtaccg gatgagctca tggagatggc
     5161 ccgggccatc aactcgatgg ccgagtcgat agagcgccgg ggcgaggagc tcgagcgcag
     5221 cgagcggcgc taccgcatga ccttcgagcg cagcggcgcg ccggttctgc tgctgtccgc
     5281 cgacgacgcg tccgtccgcg acgcgaaccc ggccgcgctc gagtactacg gctacgcgcc
     5341 ggaggagatc aggcggctcg ggatagccga cctcgacgcc tcgggccgta aggtccaggc
     5401 gtataagggc gccgacaggc tgagctcgcg gcaccgcctg agctccgggg agatacgcga
     5461 cgtgatcctg tacgtcagcc cgctcgagct cggcgacgag gagctgctcc acgctctggt
     5521 cttcgacgtg accgaccaga ggatagccga ggaacgtagc gtcgcggccc tcgaggagaa
     5581 gaccctgctc ctgcgcgagg tctaccaccg cgtcaagaac aacctccagg tcatctcgag
     5641 ccttttgtcc atgcaggcgg ccgtgaccgc ggaccgatcc gtgtcgacgg cgctgcaggg
     5701 cgcccaggac agggtcttcg cgatgtcgct ggcccacgag atcgtctatc agatgccgga
     5761 catctcctcg gtcgacgcgg gcgactacgc gtcccgcctc gtcgcctacc tggcggacga
     5821 gtacaaggta ccccgcgata gcgtcctggc cgcctacgaa ccgatgccgc tgagcctcga
     5881 gcgagccgtg cccttcggac tgctcctcgg cgagatcgta tcgaacgcgc tgaagtacgg
     5941 cataccgccg ggaaccgatg gcagggtccg cgtcagcttg tcgctggacg aggccgggcg
     6001 ggacaggatg gtctgcctgg tcgtcgagga cgacggaccc ggcctgccgc ccgaggtggc
     6061 cgacaagggc tcgctcggca tctcactgat cgccggcctt gccgatcagc tccgcgggac
     6121 cgtcgcctgg gagcgcccga gcgggggcgg cacccgcgtg acggtccgct tcgactcgtc
     6181 cgccgacggg aaggcccgat gacggccggg acgaggctcg ccgcgatggc tatggccgtg
     6241 gccgtatcgg ccgccgcctg cgaccagcgc cccatcgtca cgctacgcta cgcccacatg
     6301 agcccgctgg acagcgtagc gggcatgcaa gccgagtact tcgccagacg cgcgcgcgag
     6361 ctgagcgaag gcaggctcgt cgtcgaggta ttccccgcgt cggtcctcgg cgacacggcg
     6421 gaacagctgg cgatggcgcg cgacggcgtc atagacatcc accatacgac cgccggcgcg
     6481 ctcgcctcgc tggtcgaggg cttcaccgtg ctggacgcgc cctaccgggt ctccgacccg
     6541 gagc
//
LOCUS       DAOPHV010000067         6505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_15368_length_6505_cov_4.640775, whole genome shotgun sequence.
ACCESSION   DAOPHV010000067 DAOPHV010000000
VERSION     DAOPHV010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15368_length_6505_cov_4.640775"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PLQ29_02935"
     CDS             <1..394
                     /locus_tag="PLQ29_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85630.1"
                     /translation="RGPRSAPKAGAGGRPTTAGKTDGRPGGKPDAKPGAAAAPAKRQR
                     LDRPGRGGPLWKVNLARERQEERKSDRRDGARRESRQSDRTSANAPARKPSGAGNGGP
                     AGKRPGGSGSGQRPAGRDSRGPSRKPRG"
     gene            complement(439..2409)
                     /locus_tag="PLQ29_02940"
     CDS             complement(439..2409)
                     /locus_tag="PLQ29_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPG85631.1"
                     /translation="MVRGEDGWFIDEGGRRLILRGCNLGGDCKTPYRARTALPSPGEL
                     YDYRAASFVGRPLPEAEAGEHLDRLARWGFNAVRLLVTWEGLEPTGPGEYDDTYYEYI
                     ERLVAMAAERGLRLFVDPHQDAWSRWTGGDGAPAWTLERVGFDVTRLHRSGAAFVHEE
                     AGDPYPRMRWPSNYSRLACQTMFTLFFAGDDYAPSLRIDGLDSRSFLQGSYLAAVARL
                     AGRLARYDAVIGVDTLNEPSDGYIGLTDLSRLQRAMAKTGPMPSPFEAMATGSGYPTE
                     VDVYGVKGLGQGVVGRETLGEHGVSAWKDGTECLWRREGVWDVACGKPVLKRPDYFAR
                     ARGAEPDFAEHYLRPFARRVGEAVSKAAGAGRSMLFIESVPTVAAPRWDAEDAKASGV
                     SGAANAGHWYDGFTLFMKRRVPFIAYDQEEERVILGRGAVRRYYAEHLGRLKRRGLVD
                     MDGAPTLIGEFGLPYDLNGAAAYRTGDYRVHVEALSAYYDAMDENLLSATIWNYTASN
                     THERGDGWNGEDLSIWCRDDHEAGRTESGDPADAGGRALAGFVRPYARATAGLPRSMC
                     FDRKSGRFEYVFEPRPAGVQARGAGPKAGTEAQALITELYVPEVQYPRGFSIRTTGCA
                     HAVEDRPGHALVLVRAEPGATRCRVVVERARA"
     gene            complement(2411..3484)
                     /locus_tag="PLQ29_02945"
     CDS             complement(2411..3484)
                     /locus_tag="PLQ29_02945"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPG85632.1"
                     /translation="MAGNGTGREADRSKALARMARVAATAAAGGAVGFAAAAIGPIAL
                     AAAAAVLVAAVALGLWNTTPIGFRPKPFYAAPAPARLRWPEAAPIRPSGRHRRLLYCV
                     HGFPSTPADYRTVVEASEARGWDLAAPLLPGCGGEPRDLRGTEWSQYLAAVRDGWAEL
                     RPRYDAACLVGTSMGGSLALVLAEETCADPALAPSAIATIGSPAALAAWLRHGLVVNP
                     LVYLARTIGAFVPSIGAGLPDPERVGEDGDGAWKGYRGLYPRQTYTLQIGLRAMERRL
                     GDVTCPVLVCHARGDRMVDFRNAAVIESGLGSDDIEAYVANMDGFGHMRHNLLLYDSQ
                     RDRVWARILDFFERRAPLTRGRA"
     gene            3617..4393
                     /locus_tag="PLQ29_02950"
     CDS             3617..4393
                     /locus_tag="PLQ29_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPG85633.1"
                     /translation="MADGAASDDAILFDDGDHRFIRLGAGSRGSKGVVQTTQYLIVDK
                     GRGVLLDPGGVHLFPGVVAAASRYISIDDIDAIFFSHQDPDVSSGIALWLGVTKAKIY
                     IAELWLRFIPHFGIVDQARIMPVEGAGKAIRLASGAELAFVPSHYMHSPAAFSLYDRR
                     ARILFSGDIGGAVFPDGQETLFVDDFAAHVRYIEGFHKRLIASNSVAKRWVENARVLG
                     PELIAPQHGAVYRGASVRAFLDWLSGLRCGVDELDALYRM"
     gene            4396..5247
                     /locus_tag="PLQ29_02955"
     CDS             4396..5247
                     /locus_tag="PLQ29_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG85634.1"
                     /translation="MGDESESNYAVAMRRFTTGYNKGVVLSSVTRHSLEILDESMERI
                     DAGLSAVVAAFEEIRATSGSTAANAERIDSMMADVLAGNAGLAAGITERMGDVERGAA
                     VAKDLGARFDGLKAKTEGVAGITGAIQDVSDRTNILAINASIEAARAGSVGKGFRIIA
                     NEVRTLAGQTSDFAKQIEASLGELTGAVGDIAARMDEFVQLFSRFRESFAEVRANSDE
                     NAGSLQRAGDFLRQISGAIREETTALGDGLHSLEAISSSVKETHAVFGGLRSSHEFLD
                     TLLDKES"
     gene            5244..6227
                     /locus_tag="PLQ29_02960"
     CDS             5244..6227
                     /locus_tag="PLQ29_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPG85635.1"
                     /translation="MTAGAGEGRLLAVVTDDRFGGVAEETAVLGPLGVELRVASCSSS
                     AEVAAACADADAVLANLAPVDAAAVAAMRRCRVVSRYGVGLDNVDLAACAARGIAVAN
                     VPGYCDAEVAEHALALLLSLTRGVPGRDAAVRGGAWNLATGQASVAGSVVGVAGFGGS
                     GLAFARAVLGLRPSRLAVWSPNLSRERLDAALGPAAAALGVELRAASFDELLSGSDFL
                     SLHLRLTAETAGLWGEAAFSRMKRGAYLVNTARGGLVEASALARALASGRLAGAGVDV
                     YDVEPPPPGHPLLSAPNCVLTDHCAYRSTRSIAELKRRCAENAARALGLRT"
BASE COUNT          900 a         2380 c         2283 g          942 t
ORIGIN      
        1 ccgcggcccc cgctccgcgc ccaaggccgg cgccggcggc aggccgacga cggccggcaa
       61 gaccgacggc aggcctggcg gcaagcccga cgccaagcct ggcgccgcgg cggctccggc
      121 gaagcgccaa cggctcgacc gtcccggccg cggcggcccc ctctggaagg tgaacctggc
      181 cagggagcgg caggaagaac gaaaatccga ccggcgcgac ggagctcggc gcgaatcgag
      241 gcagtccgac cggacgagcg ccaacgcgcc cgccaggaaa ccgtccggag ccgggaacgg
      301 cggcccggct ggcaagcggc ccggcggttc aggctcaggt cagcgcccgg ccggccgcga
      361 ctcccgcggc ccctcgcgca agccgcgcgg ctgaggcggc ttgcgctgct gaggcggctt
      421 gcgctgctga ggcggcggct aggcccgggc gcgttcgacg acgacccggc aacgggtagc
      481 gccgggctcg gcccgcacga gtacgagcgc gtgtcccggc cggtcctcga cggcgtgggc
      541 gcagcccgtc gtccgtatcg agaagccgcg cgggtactgg acctcgggca cgtacagctc
      601 ggtgatcagg gcctgggcct cggttccggc cttggggccg gccccgcgcg cctggacgcc
      661 ggccggtcgg ggctcgaaga cgtactcgaa gcggccgctc ttccggtcaa agcacatcga
      721 ccggggcagg ccggccgtgg cccgggcgta gggccgcacg aagccggcca gcgcccttcc
      781 accggcgtcg gccgggtcgc ctgactcggt ccggccggcc tcgtggtcgt cgcggcacca
      841 gatcgacagg tcctcgccgt tccagccgtc gccgcgctcg tgcgtgttgc tcgccgtgta
      901 gttccagatc gtcgctgaca gcaggttctc gtccatcgcg tcgtagtacg ccgacagggc
      961 ctccacgtgc acgcggtagt cgcccgtgcg gtaggccgcg gccccgttca ggtcgtaggg
     1021 cagcccgaac tcgcctatca gcgtcggcgc gccgtccatg tcgacgaggc cgcggcgctt
     1081 gagccggcca agatgctcgg catagtagcg cctgaccgcc ccccggccca ggatgacgcg
     1141 ctcctcctcc tggtcgtagg cgatgaaggg cacgcgccgc ttcatgaaca gggtgaagcc
     1201 gtcgtaccag tggccggcgt tcgccgcgcc cgacacgccc gaggccttcg cgtcttcggc
     1261 gtcccatcga ggggccgcca cggtcggcac gctctcgatg aacagcatgg agcggcccgc
     1321 cccggcggcc ttcgacaccg cctcgccgac ccgcctcgcg aagggccgca gatagtgctc
     1381 ggcgaaatcc ggctcggcgc cgcgcgcccg ggcgaagtag tccgggcgct tgagcacggg
     1441 cttgccgcag gcgacgtccc agacgccctc gcgccgccag aggcattccg ttccgtcctt
     1501 ccaggcggag acgccgtgct cgccgagcgt ctcgcggccg acgaccccct gcccgaggcc
     1561 cttgaccccg tagacgtcca cctcggtcgg gtagccggat cccgtcgcca tcgcctcgaa
     1621 gggagagggc atcgggccgg tcttggccat cgcccgctgg agcctcgaca ggtcggtcag
     1681 gcctatgtag ccgtcggacg gctcgttgag ggtatcgacg cctatgaccg cgtcgtagcg
     1741 ggccagccgg ccggccagcc tggccacggc ggctaggtag ctgccctgca ggaaactcct
     1801 ggaatcgagc ccgtcgatcc ggaggctcgg cgcgtagtcg tcgccggcga agaacagcgt
     1861 gaacatggtc tggcaggcca ggcgggagta gttcgacggc cagcgcatgc gcgggtaggg
     1921 atcgcccgcc tcctcgtgga cgaaggccgc tccggagcgg tggaggcgcg ttacatcgaa
     1981 gccgacccgc tcgagggtcc aggccggcgc gccgtcgccg ccggtccagc ggctccaggc
     2041 gtcctggtgc gggtcgacga acaggcggag gccgcgctcg gcggccatcg cgacgaggcg
     2101 ctctatatac tcgtaatacg tatcgtcgta ttcgcctggc ccggtcggct cgaggccctc
     2161 ccaggtgacg aggagcctga ccgcgttgaa gccccagcgc gccaggcggt ccaggtgctc
     2221 cccggcctcg gcctcgggca gcggccgccc cacgaagctc gcggcgcggt agtcgtacag
     2281 ctcgcccggg ctcggtagcg cggtacgcgc gcggtacggc gtcttgcagt cgccgcccag
     2341 gttgcagccg cgcaggatca gcctcctgcc gccctcgtcg atgaaccaac cgtcctcgcc
     2401 ccgtaccatg ctaggccctc cccctcgtca gcggcgcccg ccgctcgaag aaatccagga
     2461 tgcgcgccca gacccggtcg cgctggctgt cgtagagcag caggttgtgc cgcatgtggc
     2521 cgaacccgtc catgttggcc acgtaggcct cgatatcgtc cgagccgagc cccgattcga
     2581 tgacggccgc gttcctgaag tcgaccatgc ggtcgcctcg ggcgtggcag accaacacgg
     2641 ggcaggtcac gtcgccgagc cgccgttcca tggctcgcag gccgatctgg agcgtgtagg
     2701 tctgcctcgg gtagagtccc cggtagcctt tccaggcgcc gtcgccgtcc tcgccgacgc
     2761 gctcgggatc gggcaggccg gcgccgatcg acggcacgaa cgcgccgatg gtccgggcca
     2821 ggtagacgag cgggttgacg acgaggccgt gccggagcca ggccgcgagc gccgccggcg
     2881 agcctatcgt cgctatcgcc gacggggcga gcgccgggtc ggcgcaggtc tcctcggcca
     2941 gcacgagcgc gagcgagccg cccatcgagg taccgaccag gcaggcggcg tcgtagcgcg
     3001 gccggagctc cgcccatccg tccctgaccg ccgcgaggta ttggctccac tcggtgccgc
     3061 gcaggtcgcg cggctcgccg ccgcagccgg gcaagagcgg cgcggccagg tcccagcccc
     3121 gtgcctcgga ggcctcgacg accgtgcggt agtccgccgg cgtcgacggg aagccgtgga
     3181 cgcagtagag gaggcggcgg tgccgtccgg atggccgtat cggcgcggcc tcgggccagc
     3241 gtaggcgcgc gggcgccggc gccgcgtaaa agggcttcgg cctgaagccg atgggcgtcg
     3301 tgttccagag cccgagggcg accgccgcta ccaggacggc cgcggcggcg gcgagggcga
     3361 tcggcccgat cgcggcggcc gcgaagccca cggcgccccc cgcggcggcc gtcgccgcga
     3421 cgcgcgccat ccgcgccaga gccttcgagc gatccgcttc gcgccccgta ccgtttcctg
     3481 ccatccgcgg accgcccccc tcgtggtacg aagcttcgct acgccgtcgc ggcgcgccga
     3541 tcgagtatac tacccatttt cggccatggt atagtatcgt cgtagtacgg gcgatgcccg
     3601 tacacggagg tgatacatgg cagacggcgc ggcgtccgac gacgccatcc tgttcgacga
     3661 tggcgaccat cgcttcatcc ggctcggcgc cggttcgcgc ggctccaagg gcgtggtcca
     3721 gacgacgcag tacctgatcg tcgacaaggg ccgcggcgtc ctgctcgacc cgggcggggt
     3781 gcacctgttc cccggggtcg tcgcggcggc cagccgctac atctcgatcg acgacatcga
     3841 cgcgatcttc ttctcgcacc aggatcccga cgtctcgagc ggcatagcgc tctggctcgg
     3901 cgtcacgaag gccaagatct atatagccga gctttggcta cgcttcatcc cgcacttcgg
     3961 catcgtcgac caggcccgca tcatgccggt cgaaggcgcc ggcaaggcca tcaggctcgc
     4021 ctcgggggcc gagctggcct tcgtgccgtc ccactacatg cactcgcccg cggctttctc
     4081 gctgtacgac cgcagggccc gtatcctctt ctccggggac ataggcggcg ccgtcttccc
     4141 cgacggccag gagacgctgt tcgtcgacga cttcgccgcc cacgtccggt atatcgaggg
     4201 cttccataaa cgcctgatag cctcgaattc cgtggccaaa cgctgggtcg agaacgcgcg
     4261 ggtcctcggc cccgagctga tcgcgcccca gcacggcgcc gtctaccgcg gcgcctcggt
     4321 ccgggccttc ctcgactggc tgtccggcct ccgctgcggc gtcgacgagc tcgacgcgct
     4381 gtacaggatg tagccgtggg cgacgaaagc gaatcgaact acgccgtagc gatgcggcgc
     4441 ttcaccaccg ggtataacaa gggcgtcgtg ctatcgtccg ttacccggca ctccctcgag
     4501 atactcgacg agtcgatgga acgcatcgac gcgggcctgt cggcggtcgt cgccgccttc
     4561 gaggagatca gggcgacgag cggctcgacc gccgccaacg ccgagcgcat cgactcgatg
     4621 atggccgacg tgctcgccgg caacgcgggc ctcgccgccg gcatcaccga gcgcatgggc
     4681 gacgtcgagc gcggcgccgc cgtggccaag gacctcggcg ccaggttcga cggcctcaag
     4741 gccaagaccg agggcgtcgc cggcatcacc ggcgccatcc aggacgtctc cgaccgcacc
     4801 aacatcctcg cgatcaacgc ctcgatcgag gccgctcgcg ccggatccgt aggcaagggc
     4861 ttccgcatca tcgcgaacga ggtccggacc ctcgcgggcc agaccagcga cttcgccaag
     4921 cagatcgagg cgagcctcgg cgagctgacc ggggccgtcg gcgacatagc ggcgcgcatg
     4981 gacgagttcg tccagctgtt cagccgcttc cgcgagtcgt tcgccgaggt cagggccaac
     5041 tccgacgaga acgccggcag cctccagcgc gccggagact tcctccggca gatatccggc
     5101 gccatccgcg aggagacgac ggcgctcggc gacgggctcc actcgctcga ggccatcagc
     5161 tcgagcgtca aggagacgca tgccgtgttc ggcggcctgc gctccagcca cgagttcctg
     5221 gatacgctcc tggacaagga gtcatgacgg ccggcgccgg ggagggccgg ctgctcgcgg
     5281 tcgtcaccga cgaccgcttc ggcggcgtgg ccgaggagac ggcggtgctc gggccgctcg
     5341 gcgtcgagct gcgcgtggcc tcgtgttcga gctctgccga ggtggcggcc gcctgcgccg
     5401 acgccgacgc cgtgctggcg aacctggcgc ccgtggacgc ggccgcggtg gccgccatgc
     5461 ggcgctgccg cgtcgtgtcg cgctacggcg tcgggctcga caacgtcgac ctggccgcct
     5521 gcgccgcccg cggcatcgcc gtggccaacg tacccggcta ctgcgacgcc gaggtggccg
     5581 agcacgccct ggccctgctc ctgtcgctga cccgcggcgt gcccgggcga gacgcggccg
     5641 tgcgcggcgg cgcctggaac ctggcgacgg gccaggcctc cgtggccggc tccgtggtcg
     5701 gggtggcggg gttcggcggc tccgggctgg ccttcgcgag ggccgtcctg ggcctccgcc
     5761 cgtcgcggct cgccgtctgg agcccgaacc tgtcccggga acggctcgac gccgccctcg
     5821 ggccggcggc cgcggccctc ggcgtcgagc tacgcgccgc gagcttcgac gagctgctct
     5881 ccgggtccga tttcctatcc ctgcacctgc gcctgacggc cgagacggcc ggcctctggg
     5941 gcgaggcggc cttctcccgg atgaagcgcg gcgcgtacct cgtcaacacg gcccgcggcg
     6001 gcctcgtcga ggcgtccgcc ctggcgcgcg ccctcgcctc cggcaggctg gctggcgccg
     6061 gagtcgacgt gtacgacgtc gagccccctc cgccgggcca tccgctgctc tccgccccga
     6121 actgcgtgct gaccgaccac tgcgcctacc gctcgacgcg ctcgatagcc gagctcaagc
     6181 gccgctgcgc cgagaacgcc gctcgcgccc tcgggctgcg tacctaggcg aggacgacga
     6241 agcgcaggct gtcccagccg tcgaccgccc cgcggacaac ggctggcgac gttgcggatc
     6301 cccggaacga tggttcgcga tcgatgcgtc atgtttcgtg atcgataggt catgtttcgc
     6361 gatcgaggtg tcatgtttcg tgatcaatgc gtcatgtttc gtgatcgatg tgtcatattt
     6421 cgtgatcaat gcgtcatgtt ccgcgatcga tgtgtcatgt ttcgcgatcg ataggtcatg
     6481 tttcgcgatc gatgagtcat attcc
//
LOCUS       DAOPHV010000068         6467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_15504_length_6467_cov_3.522614, whole genome shotgun sequence.
ACCESSION   DAOPHV010000068 DAOPHV010000000
VERSION     DAOPHV010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15504_length_6467_cov_3.522614"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLQ29_02965"
     CDS             <1..505
                     /locus_tag="PLQ29_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HPG85636.1"
                     /translation="TGNLGDVMKESARAALTYLRAHAEEYGLSPTDFAGRVIHVHVPE
                     GAIPKDGPSAGITLAAAIASALTGSPLSAGWAMTGEITLTGRILAIGGVKEKILSAHR
                     NKIYKALLPEANRKDLDDIPSEVSQAMEFRFCSTIAEAFAVLLPSATPRSPGRPGKAA
                     APRKRPE"
     gene            513..854
                     /locus_tag="PLQ29_02970"
     CDS             513..854
                     /locus_tag="PLQ29_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85637.1"
                     /translation="MKGDVANLTRAMLARLSFAAVAVAAVAVSIYISGSLGSASSEAL
                     SLSSLAAGVAGLLAVGFAVGAIVGTALAPAVGARFFNRTVVGSLAAAALGAAIAATMA
                     VVGAASGGLSF"
     gene            complement(851..1753)
                     /locus_tag="PLQ29_02975"
     CDS             complement(851..1753)
                     /locus_tag="PLQ29_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85638.1"
                     /translation="MSIKRRFDGTMIDVPAFSRMMLFVMPRKSDALVYFEHEVDVTET
                     LEYVHEINRELTKRKSVLTLFELVMCAAVRTIVERPRLNRFVSDYRFYQRNRIEFSFV
                     AKKELSDDGEEVTVKIPYSPFETLESLAVKTKRFIRRAVSDDGMTTEKIIEFVARLPN
                     WMVRLIAGSVDFLDAKRVPLGDLVKTDPMWCSIFFTNVGSFNLDAPYHHLYERGNCPL
                     FVSVGVVRTVRETGPDGELRERKKLTLRYTCDERISEGVYMAKALELMKGYIENPRPL
                     AEAPAIGEAAMVELAIDPSTYSEN"
     gene            complement(1835..2920)
                     /locus_tag="PLQ29_02980"
     CDS             complement(1835..2920)
                     /locus_tag="PLQ29_02980"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPG85639.1"
                     /translation="MVHSLLKIGAAAIVSVALASLLSSCWYAKQAAHFLAERASAVPV
                     ERVASNPGTPPETVAMLETVDRVRRFSVDRVGLSPTRNYTRYVALDRDYVADVVSACA
                     ADSFERYRWRYPVLGELPYKGFYDKADANREAKRLKEAGLDVIVRQVEAFSSLGYFKD
                     PLYSFMVGSDEDELAELVIHESAHATLFVKGADQFNEGFATFVGRRGAELYLDERYGP
                     DSTQAARRRARMADGRAFVGFIRGTAALLEEAYADESVGRDARLERKAAVIAERASLF
                     AAERERLFDGEAYRRFDMSTINNAYIDLYRLYEDDLSLFDAWFDRVAGGSLPVFVSAL
                     SALALTEKKRVTMAMAERLGLASDAVP"
     gene            2985..5141
                     /locus_tag="PLQ29_02985"
     CDS             2985..5141
                     /locus_tag="PLQ29_02985"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPG85640.1"
                     /translation="MARKEQPTGAARYEALLFITAAIWGTGFVAQRLGMVSLGPFSYN
                     AARFMLGSTILVPVLVARRASQAAFKAALGPSMMAGTILAAAASLQQAGLQYTSAGKA
                     GFITGLYVVLVPVAAMVLGRRTPMRTWLGAAFAFGGLYALSFSSIAGVNSGDLLVLAS
                     SVFWTAHILALDRWASRVEPLMLACIQFAVCSSWCWLGALAFESPSITDFTAGAAPIA
                     YGGVLSIGVAYTLQAVAQSKAHPARAAIIMALESPFAAVSGALLLGERMSPGETAGCA
                     LMLAGTFVAQWPARKAASLRLASGPGSLYRVGMETTIPLLHDNHNHVALYAAFASCLD
                     ISSLPPDEALSTLRALPSGTLSVVRGWKSFELPMGRETLSSLPPVLLINFSLHGFVVS
                     DSAIPYLERVVPEVAARRDDQAWCEANVPAIFGAYCGLAGLDEDKLVSYIDAMVRLGI
                     GSTDEMTVPTLEALDACLASRYADRLRLWVSPALYRKLDKSRRGRVMGVKLYLDGAIG
                     ARSAAIVGPWIGSGKAMFTYRDDELVAVLEEIASYGTGVSMHAIGELAIAQAIGSLER
                     LGPSAWPLVRLEHLQFIDRAMADRAKKLGATLSMQPNFTSDSRDYADRLAGAYLKGNN
                     PFRMLIDDAGFVPGADLLFGSDGMPDGIAYAATQALFPPFPSQRLSLEELVAGYGEAI
                     GIAGFARLRIDEEARRVTVLEARQGSSSPKTMAVNR"
     gene            complement(5099..5665)
                     /gene="amrA"
                     /locus_tag="PLQ29_02990"
     CDS             complement(5099..5665)
                     /gene="amrA"
                     /locus_tag="PLQ29_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPG85641.1"
                     /translation="MSLELSNEDKGALLATAREAIESRLLGRQPRWPSGGPALQVRLG
                     AFVTLHKRSSYGRRLLRGCIGRMSADKALVLTVRDMALAAAFEDPRFPPLIDRELEAI
                     DVEISVLSPFEPCAPDDVVPGVHGVLLRRGYSSGVFLPQVATEQGWDREALLEELCVK
                     AGVESGSYRAPDASLYRFTAMVFGELEP"
     gene            complement(5662..>6467)
                     /gene="amrB"
                     /locus_tag="PLQ29_02995"
     CDS             complement(5662..>6467)
                     /gene="amrB"
                     /locus_tag="PLQ29_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPG85642.1"
                     /translation="SSPVGSSSPVGSSSPVGSSTAMVGKALAILAPHAAFDYSIDLQA
                     MSWLAASMSLVDAAPDRVAVIGPTRRCDEHGVYLPEVEAFQTPLGDIEVDVEACEELE
                     SCCTLFSRNDIPHFESHSIELQLPFIRLLFPGARLIPILACGDASVAAGLARSIDAVL
                     GADLGRTLIVASSNMAASIVAADSSRRTDELLQSIARGAWRDVASRDDIGGATAIAAA
                     MAVAPMQDRRFALFGRGDSLGHEKSAAERVVQYASAAWYRLAASPAEAP"
BASE COUNT         1099 a         2121 c         2097 g         1150 t
ORIGIN      
        1 gacgggcaac ctcggcgacg tgatgaagga gagcgccagg gcggcgctga cctatctgcg
       61 ggcccacgcc gaggagtacg gtctgtcgcc gacggacttc gctggccgcg tcatccacgt
      121 tcacgtcccc gagggtgcca taccgaagga cggcccgtcg gccggtatca cgctggccgc
      181 cgccatagcc tcggccctga ccggttctcc gctctcggcc ggctgggcga tgaccggcga
      241 gatcaccctg accgggcgca tcctggccat cggcggcgtc aaggagaaga tcctctcggc
      301 ccataggaac aagatatata aggcgctcct gcccgaggcc aaccgcaagg acctcgacga
      361 catcccctcc gaggtgtcgc aggcgatgga attccgtttc tgctcgacga tagcggaagc
      421 gttcgcggtg ctgttgccgt cagcgacgcc ccggtcgccc ggacggcccg ggaaagcggc
      481 cgctccccgc aagcggccgg agtaggccca ccatgaaggg cgacgtagcc aacctgacgc
      541 gggccatgct cgcgcgccta tccttcgcgg ccgtggccgt agcggccgta gccgtttcga
      601 tatatatatc cggcagtctc ggctccgctt cctccgaggc tctgtcacta tcgtcgctgg
      661 ccgccggcgt agcgggcctg ttggcggtcg gtttcgccgt cggcgccatc gtcggcacgg
      721 cgctcgcccc ggcggtcgga gccaggttct tcaaccggac cgtcgtcggg tcgctcgccg
      781 cggcggcgtt aggcgccgcc attgccgcta cgatggcggt cgtcggagcc gcgtcgggcg
      841 gcctgtcgtt ctagttctcc gaataggtgg acggatcgat ggccagctcg accatggcgg
      901 cctcgccgat ggctggagcc tcggccagcg gccgtgggtt ctcgatatag cccttcatca
      961 gctcgagcgc cttggccatg tagacaccct ctgatatgcg ctcgtcgcag gtatagcgta
     1021 gcgtcagctt cttccgctcc cgtagctcgc catcggggcc ggtttcgcgc acggtacgca
     1081 ccacgccgac cgatacgaac agcggacagt tcccgcgctc gtacaggtgg tggtacggcg
     1141 cgtcgaggtt gaacgagccg acgttcgtga agaaaatgga gcaccacatc ggatcggtct
     1201 tgacgaggtc tccgagcggc acgcgcttgg cgtcgaggaa atcgacgctc ccggctatca
     1261 gcctgaccat ccagttcggt agcctggcca cgaattcaat gatcttctcg gtcgtcatgc
     1321 cgtcgtccga cacggcccgc cggatgaagc gtttggtctt gaccgccagg ctctccaggg
     1381 tctcgaaggg cgagtagggt atcttgacgg taacctcctc accgtcgtcg ctcagctcct
     1441 tcttggccac gaagctgaac tcgatgcggt tcctctggta aaagcgataa tcggaaacga
     1501 agcggttcag gcgagggcgc tcgacgatgg tcctgaccgc ggcgcacatg accagctcga
     1561 acagggtcag caccgacttg cgtttcgtca gctcgcgatt gatctcgtgc acgtactcga
     1621 gagtctccgt cacgtcgacc tcgtgctcga aatagaccag ggcgtcgctc ttgcggggca
     1681 taacgaacag catcatgcga gagaaagccg gcacgtctat catggtgccg tcgaaacgtc
     1741 gctttatgga cacggttcaa atcctttcgg cgggcggctt tcccgggtgg atatccgtcc
     1801 acatgaacat gctattttcc tatgacgtct tgtatcacgg aaccgcatcg ctagccaagc
     1861 cgagtcgctc ggccatggcc atcgtgacgc gcttcttctc ggtaagcgcc agggcgctta
     1921 gggcggagac gaatacaggt agcgatcctc cggcgacccg gtcgaaccag gcgtcgaaga
     1981 ggctcaggtc gtcctcgtag agccggtaga ggtctatata ggcgttgttg atcgtcgaca
     2041 tgtcgaagcg acgataggcc tcgccatcga ataggcgctc ccgctcggcg gcgaacagcg
     2101 acgccctctc ggctatcacg gcggccttgc gctcgagcct cgcgtcgcgg ccgaccgact
     2161 cgtcggcgta tgcctcctcg agcagagcgg ccgtaccgcg tataaaaccg acgaaggcac
     2221 gcccgtcggc catcctcgct ctccgcctcg cggcctgggt cgagtccggg ccgtagcgct
     2281 catcgaggta cagctcggcg ccgcgcctac cgacgaaggt ggcgaagccc tcgttgaact
     2341 ggtctgcccc cttgacgaac agggtcgcgt gcgccgattc gtggatcacc agctcggcaa
     2401 gctcgtcctc gtcgctcccg accatgaacg aatagagcgg atccttgaag tatccgagcg
     2461 agctgaaggc ctcgacctgc ctgacgatga cgtcgagccc cgcctccttg agccgcttcg
     2521 cctcgcggtt agcgtcggcc ttgtcgtaga aacccttgta cggtagctcg ccgagtacgg
     2581 gatatcgcca gcgatagcgt tcgaaggagt ctgccgcgca ggcagagacg acgtccgcca
     2641 cgtagtcgcg gtcgagagcc acgtagcgcg tgtaattcct ggtaggggac aggccgacgc
     2701 ggtccacgga gaaacgcctg acccgatcga ccgtctcgag catggcaacg gtctcaggcg
     2761 gtgtgcccgg attggaggcg acccgctcga ccggcacggc cgaggcgcgc tcggccagga
     2821 aatgcgccgc ctgtttggcg taccagcacg acgataggag ggacgccagg gcgacggaga
     2881 cgatagccgc ggcgcctatc ttgagtagcg aatgaaccat agccgattgt agcaccgttc
     2941 aggacacctt gcacccgtct gaacggcgct gatagactcc catcatggca cgtaaggaac
     3001 agccgaccgg agccgcccgt tacgaggcgc tgctcttcat caccgcggcc atctggggca
     3061 ccggtttcgt cgctcaaagg ctcgggatgg tcagcctcgg tccgttctcg tacaacgcgg
     3121 ccaggtttat gctcggctcg acgatactcg tgccggtatt ggtagcgagg cgcgcctcgc
     3181 aggccgcgtt caaggcagcc ctgggaccga gcatgatggc cggaaccata ttggccgcgg
     3241 cggccagcct acagcaggcg gggctccagt atacaagcgc cggtaaggcg ggctttatca
     3301 ccggcttgta cgtggtgctc gtaccggtcg ccgccatggt tctcgggcgc aggacgccga
     3361 tgcgaacctg gctaggcgcc gcgttcgcct tcggcggcct ctacgcgctg tcgttctcgt
     3421 caattgccgg cgtcaactct ggcgacctcc tcgtgctcgc cagctccgtg ttttggaccg
     3481 cgcatattct cgccctcgac cgctgggcct ctcgggtcga gccgctgatg ctggcctgca
     3541 ttcagttcgc cgtgtgctcc tcgtggtgct ggctgggagc cttggcgttc gaatcgccgt
     3601 caatcacgga ttttaccgcg ggcgcggctc cgatagccta tggcggcgta ctgagcatag
     3661 gcgtcgcgta cacgttgcag gccgtggcgc agagcaaagc ccacccagcc cgcgccgcca
     3721 tcatcatggc gctcgagtct ccattcgcgg ccgtatcggg agccctactc ctcggcgaac
     3781 gcatgagccc gggcgagact gccggctgcg ccctgatgct agccggtacc ttcgtggcgc
     3841 agtggccggc caggaaagcc gcctcgctcc gacttgcgtc gggaccaggt tcgctatatc
     3901 gtgtcggcat ggaaacgact atccccttat tgcacgataa ccataaccac gtcgctctct
     3961 atgcagcgtt cgcgtcctgc ctcgacattt cgagcctgcc gcccgacgag gcgctctcga
     4021 cgctgcgggc ccttccgtcc gggacactat cagtcgtcag gggctggaaa tccttcgagt
     4081 tgcccatggg ccgcgagacc ctgtcgtcgt tgccgcccgt cctcttgatc aatttcagcc
     4141 tgcacggctt cgtcgtcagc gactcggcga taccgtacct cgagcgggtc gtgcccgagg
     4201 tcgccgccag gcgagacgat caggcctggt gcgaggcgaa cgtaccggca attttcggcg
     4261 cgtactgcgg cctggccggc ctcgacgagg acaagctcgt ctcctatatc gacgcgatgg
     4321 ttcgcctcgg tatcggatcg accgacgaga tgaccgtacc gacgctcgaa gccctcgacg
     4381 cgtgcctggc ttcgcgctac gccgataggc tcaggctatg ggtatcgcca gcgttgtata
     4441 gaaaactgga caagtcgagg cgcggacgcg tgatgggcgt caagctgtat ctggacggcg
     4501 ccataggcgc caggagcgcc gctattgtcg gaccgtggat agggtctggc aaggctatgt
     4561 tcacctatag agacgacgag ctcgtagccg ttctcgagga gatcgcttca tacggtacgg
     4621 gcgtgtccat gcacgcgatc ggcgagctgg ccatagccca ggccatagga tcgctcgaga
     4681 ggcttggccc ctccgcctgg cccctcgtca ggctggaaca tctgcagttc atcgacaggg
     4741 ccatggccga tcgcgccaag aagctcggcg cgaccctgtc gatgcagcct aatttcacga
     4801 gcgactcgcg cgattatgcg gatcgactcg ccggggccta cctgaaaggg aacaatccgt
     4861 tcaggatgct catcgacgac gccggcttcg taccgggcgc ggatctgctg ttcggctccg
     4921 acggcatgcc ggacggcata gcctacgcgg ccactcaggc gctgttcccg ccgttcccgt
     4981 cccagcgcct gagcctcgag gaactggtag ccggctacgg agaggccatc ggcatagcgg
     5041 gcttcgcgcg gctacggatc gacgaggagg cgcggcgcgt taccgtgctc gaggcccgtc
     5101 agggctcgag ttccccgaag accatggcgg taaatcgata gagactcgcg tcaggcgccc
     5161 tgtaggagcc gctttcgacg ccggccttga cgcatagctc ctcgagcaga gcctcgcgat
     5221 cccagccctg ctctgtggcg acctggggca ggaagacgcc ggaggaataa ccccgcctca
     5281 ggagcacccc atgcacgcct gggacgacgt catccggagc gcatggctcg aagggcgaca
     5341 gcaccgaaat ctcgacgtct atagcctcca gctcgcggtc gatcaggggc ggaaagcgcg
     5401 gatcctcgaa ggcggcggcc aacgccatgt cgcgcacggt aaggacgagg gccttgtcgg
     5461 cgctcatcct gcctatgcag ccgcgtagca gccgccggcc gtacgacgac cgcttgtgca
     5521 gggtgacgaa ggcgccgagc cgaacctgga gggcgggccc gccgctcggc cagcggggct
     5581 ggcggccaag cagtcgggac tcgatggctt cccgcgccgt cgcgagtagc gcgcccttgt
     5641 cttcgttcga tagctcgagg ctcatggcgc ctccgcgggc gaggcggcga gccggtacca
     5701 ggccgccgag gcgtactgca ccacgcgctc ggcggccgac ttctcgtggc caagcgaatc
     5761 gccccggccg aacagcgcga agcgccgatc ctgcatcggc gcgacggcca tggccgcggc
     5821 tatggccgtc gcgccgccta tatcgtcgcg tgacgccacg tcccgccagg cacctcgagc
     5881 gatgctctgt aagagctcgt cggtacgcct ggacgagtcg gcggcgacga tggacgccgc
     5941 catgttgctc gaggcgacga tgagggtccg cccaaggtcg gctccgagca cggcgtcgat
     6001 ggaccttgcc aggcctgcgg cgactgaggc gtcgccgcag gccagtatcg gaatgagcct
     6061 ggctcccggg aaaaggagcc gtatgaatgg cagctggagc tctatggaat gactctcgaa
     6121 atgcgggata tcgttgcgcg agaacagggt gcagcacgac tcgagctcct cgcaggcctc
     6181 gacgtccacc tcgatatccc cgagcggcgt ctggaacgcc tcaacctccg gtaggtatac
     6241 accgtgctcg tcgcaacgcc tggtcggccc gatcacggca acgcgatcgg gggcggcgtc
     6301 gacgaggctc atggacgccg ccagccagct catggcctgg aggtcgatgg agtagtcgaa
     6361 ggccgcgtgc ggcgcgagga tggccagggc cttaccgacc atcgccgtcg acgagcctac
     6421 tgggctcgat gagcctactg ggctcgatga gcctactggg ctcgatg
//
LOCUS       DAOPHV010000069         6408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_15720_length_6408_cov_3.468598, whole genome shotgun sequence.
ACCESSION   DAOPHV010000069 DAOPHV010000000
VERSION     DAOPHV010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15720_length_6408_cov_3.468598"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..482
                     /locus_tag="PLQ29_03000"
     CDS             255..482
                     /locus_tag="PLQ29_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85643.1"
                     /translation="MNEHGRKMVAPAVIVICLVAYYAAVALLILKLELPRLASVGIVV
                     FSALLSGILIWTLVDRIKEIRKGEEDDLSKY"
     gene            463..777
                     /locus_tag="PLQ29_03005"
     CDS             463..777
                     /locus_tag="PLQ29_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HPG85644.1"
                     /translation="MTLVNTDYISGKELETLGLVKGSTIQSKNIGKDILQAFKSMVGG
                     ELRAYNEMMNEARALATKRMVEEAESLGADAVVNIRYASSSIMQNAAEVIAYGTAVKY
                     RA"
     gene            811..1260
                     /locus_tag="PLQ29_03010"
     CDS             811..1260
                     /locus_tag="PLQ29_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPG85645.1"
                     /translation="MYPVRNSVKALIIKDGSLLCVRKRDEYGAYYLLPGGGQEKGETF
                     PETLRRECLEELGAAVRVGVLRYIREYIGRNHEFAATDDAHQVEFMFECELLGAPDSA
                     LASHVDEGQDGIAWVPLVGAGSERIYPRVLPGRLRDGCAELYWGDVN"
     gene            1298..1759
                     /locus_tag="PLQ29_03015"
     CDS             1298..1759
                     /locus_tag="PLQ29_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HPG85646.1"
                     /translation="MILVSACLAGLRCKWNLESAEHPAMSRLFREGMAIPVCPEQLGG
                     LSTPREPSELRDGRVFAKGGRDVTREYRRGAEETLAIARAFDCRSAVLKSRSPSCGCG
                     RVYDGTFTGRLVPGDGVTAALLKANGIAVYDESAAGEVAAAIGVSTVAEEN"
     gene            1763..2659
                     /locus_tag="PLQ29_03020"
     CDS             1763..2659
                     /locus_tag="PLQ29_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HPG85647.1"
                     /translation="MGIRFYQVDSFATRALEGNPAGVVPDTRGLDETTMRRVAREINA
                     SETAFVLEKPGDGYDVEIRFFTPTREVPVCGHATVAAHYVRALERGYRGGVVRMRTKA
                     GILPVEVLPEGDDLRVVMTQGAIEFGPVLEGDDRTALLAGLGVGPADLEPGLPIRIVS
                     TGHGKVMVPLAGRARLDALEPDDSALAALSGRIGCNGFYAFSLDSGEPGVLASGRMFA
                     PAIGIREDPVTGNANGPLGAYLTRYGRIPALADGYSFRIKQGEAMDRKGYMDVAVFCE
                     DGEPVRVTIGGRAHVAFEATLS"
     gene            complement(2713..3933)
                     /gene="tyrS"
                     /locus_tag="PLQ29_03025"
     CDS             complement(2713..3933)
                     /gene="tyrS"
                     /locus_tag="PLQ29_03025"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPG85648.1"
                     /translation="MDAKKVAERQLRLLERGCDEIVPREALLEKLERSVETGRPLRVK
                     LGIDPTGNAVHIGHMVPMGKLRQFQDLGHMAVLIIGDYTASIGDPTGRNAERPPLSLE
                     RVRLNAERYAEQIFRVVDGRKAELRWQSEWYGSLSLAELLGLAGSFSLAQLLAHETFR
                     KRYDDGARVGLHELLYPVLQAYDSVMVGADVELGGTDQKFNVLAGRELQRDRGLDAQC
                     AMLMPLLPGIDGRKMSKSLGNDIGVDAGPDEQFARVMAIRDEAMDAYRELVLLGTPED
                     SAEAASRLAAGENPMRLKLELASGIVERFNGQGSGRAARAAWERRFSRREAPDDVPLY
                     ACDRATDLRSLMRDAGLASSSSEAARLVAEGAVYSGDTRLSDPGFRIDPDELPEEGLT
                     IRVGRRRYLRVVRG"
     gene            complement(4193..5440)
                     /locus_tag="PLQ29_03030"
     CDS             complement(4193..5440)
                     /locus_tag="PLQ29_03030"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HPG85649.1"
                     /translation="MIDTELDIAWILREHGERDLSASRELFPIEMARKSRKFHRQIPG
                     YRTSPLLALPHLSAMFGVGGIWVKDEASRMELNSFKVLGGSFALYRFIQERLGIPDEK
                     MSYEYLLSKEVKDRLGSITFASATDGNHGRGIAWAAQKLGHPCVIYVHAETSRRRIDA
                     IRGYGATVKIIEGTYDDAVRQIAVDSAKYGWEIISDTSWDGYTAVPTWIMQGYTTMLA
                     EAQEQFSAQGVVKPTHIFVQAGVGALAAAVTGYYHNLFGPDAPVCVVVEPDNAACLFH
                     SAEVDDGKPHSVGGALDTIMAGLACGEPSPIAWDVLRETVDAFVTVPDYVAAKGMRIY
                     ATPLKGDPFIVSGESGAVTLGALVTIMQEKGAKALKEALRLDEDSQVLLINSEGNTDP
                     VQFRRIIWEGSNPVPRQHWSERD"
     gene            5536..6072
                     /locus_tag="PLQ29_03035"
     CDS             5536..6072
                     /locus_tag="PLQ29_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPG85650.1"
                     /translation="MNETKEKSQWRGRIAAVTTFKYLSPSELDFLIDKAAVVTCRPEE
                     TIVREDEVSQYFFAILDGTVNVTVDEASLDGSLRSVYICTLGPGDVFGEAGIFIKVKR
                     TATVSAADEVTLLRLHRDDMSAFIRSKPAGGNKLLLVIIYSLLRKLRAANQELAYERK
                     ADMDQADVDSLMADMLGD"
     gene            complement(6088..>6408)
                     /locus_tag="PLQ29_03040"
     CDS             complement(6088..>6408)
                     /locus_tag="PLQ29_03040"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85651.1"
                     /translation="QRDASTPGNVPASAAGVEASRCRWCEEPPCAKACPAGIDVPGAL
                     RRLECGNEAGAARRVAESDRGRPGREPGRVSCVGCAAPCLEVCVRHDVDGRPVAIAEL
                     LSGL"
BASE COUNT         1026 a         2076 c         2213 g         1093 t
ORIGIN      
        1 aaggcgcacg agctcctgca cacgagctac aagaagctgg aactgtacaa gtaattgccg
       61 gacgttgaag ccaatataaa ccgctatgcg gtttttacgg cttaacgtcc tggtgcggtt
      121 cgtatcgccc gggcctcgcg gcccgggcgt tcttattgta ggaggaacga tacgccggct
      181 cggatcgctg gcgcggtcct gcggcgtatc gtaccgggcg ggtttgtgta tagtataggc
      241 gacggaggcc ggatatgaac gagcatggac ggaagatggt tgcgccggcg gtgatcgtga
      301 tctgtcttgt ggcgtattat gcggcggtgg cgctattgat actcaagctg gagctcccga
      361 ggctcgcgag cgtcggtatc gtagtatttt ccgctctcct atcgggtata ctgatctgga
      421 cgctggtcga caggatcaag gagatccgga agggggaaga ggatgacctt agtaaatact
      481 gattatattt ccggcaagga actcgagacg ctggggctcg tcaagggtag cactatccag
      541 tccaagaata tcggcaagga tattctgcag gccttcaagt cgatggtcgg cggcgagctg
      601 cgggcgtaca acgagatgat gaacgaggcc agggcgctgg cgacgaagcg gatggtcgag
      661 gaagccgagt cgctcggcgc cgacgcggtg gtgaatatcc gctacgcgtc ctcctctatc
      721 atgcagaacg ccgccgaggt catcgcgtac ggtaccgcgg tcaagtatcg cgcgtgacga
      781 agtccggagc gcgatagcgc ggagacggcg atgtacccgg tcaggaattc ggtaaaggcg
      841 ttgattatca aggacggaag cctgctctgc gtccgcaagc gcgacgagta cggcgcgtat
      901 tacctgcttc ccggcggcgg gcaggagaag ggcgagacgt tccccgagac gctcaggcgc
      961 gaatgcctcg aggagctggg cgccgcggtc agggtcgggg ttctgcgcta tattcgggag
     1021 tacatcgggc gtaaccacga gttcgcggcg accgacgacg cgcaccaggt cgagttcatg
     1081 ttcgagtgcg agctactcgg tgcgcccgac tcggcccttg cctcgcatgt cgacgagggg
     1141 caggacggca tagcctgggt gcctctcgtc ggggccggta gcgagcggat ctatccgcgg
     1201 gtgctgcccg ggcgtctgcg cgacggttgc gccgagctgt actggggcga cgtcaattag
     1261 cggcgcatcg agcgcagcgc cgattggggg atgatcgatg atactcgtca gcgcctgcct
     1321 cgcgggcctt cgatgcaaat ggaacctcga atcggccgag cacccggcga tgtcgcgcct
     1381 gttcagggag gggatggcca tcccggtctg cccggagcag ctcggcggcc tatcgacgcc
     1441 gcgcgagccg tccgagttgc gggacggccg cgtgttcgcc aagggcggcc gcgacgtgac
     1501 gagggagtat cgtcggggcg ccgaggaaac gcttgccatc gcgagggcct tcgattgccg
     1561 atccgccgtc ctcaagtcgc gctcgccgtc ctgcggctgc ggccgcgtct acgacggcac
     1621 gttcacgggt cggctcgtgc ctggcgacgg ggtgacggcc gcgctgctca aggccaatgg
     1681 gatcgcggtg tacgacgagt cggccgctgg cgaggtggcc gccgctatcg gcgtatcaac
     1741 cgtcgcggag gagaactagc gcatgggcat acgcttctac caggtggact cgttcgcgac
     1801 gcgcgcgctc gaggggaacc cggccggcgt ggtgccggac acgcgcggac tcgacgagac
     1861 gacgatgcgc cgcgtcgccc gcgagatcaa cgcgtccgag acggccttcg tgctcgagaa
     1921 gcccggcgac ggctacgacg tcgagatccg cttcttcacg ccgacgcggg aggtcccggt
     1981 ctgcggccac gcgacggtgg cggcccacta cgtgcgcgcg ctcgagcgcg gctaccgtgg
     2041 cggcgtcgtt cggatgagga ctaaggcggg gatcctgccc gtggaggtgc tgccggaggg
     2101 cgacgacctg cgggtcgtga tgacccaggg cgcgatcgag ttcggcccag tcctcgaggg
     2161 agacgatagg acggccctgc tggccggcct cggcgtcgga ccggcggacc tcgagccggg
     2221 gctgccgatc cggatcgtat ccaccggcca cggcaaggtg atggtaccgc tcgcgggccg
     2281 ggctaggctc gacgcgctcg aaccggacga ctccgcgctc gcggccctga gcgggcgcat
     2341 cggctgcaac ggcttctacg cgttctcgct cgactcgggg gagccgggcg tgctggcgag
     2401 cggccggatg ttcgcgccgg cgatcggcat acgcgaggac ccggtcacgg gcaacgccaa
     2461 cggcccgctg ggcgcctatc tgacccgcta tggccgcatc ccagcgctag ccgacggtta
     2521 tagcttcagg atcaagcagg gcgaggcgat ggaccgcaag ggctatatgg acgtggccgt
     2581 cttttgcgag gacggcgagc ccgttcgcgt gacgataggc ggccgcgccc acgtggcttt
     2641 cgaggccacg ctgtcgtagc cctcctgcct cgcgggccga ccgccaaccg tctccgagtc
     2701 cgatcccggc ggctagcccc gcaccaccct gaggtagcgg cgcctgccga cccgtatcgt
     2761 gagcccctcc tcgggtagct cgtccgggtc gatgcggaag cccgggtcgg acagtcgcgt
     2821 atcgcccgag tagacggcgc cctcggccac gagcctggcc gcttcgctcg acgacgaggc
     2881 gaggcccgcg tcgcgcatca gcgagcgcag gtcggtcgcg cggtcgcatg cgtagagcgg
     2941 aacgtcgtcg ggcgcctcgc gcctcgagaa gcgccgctcc caggccgctc gcgccgcccg
     3001 ccccgaaccc tggccgttga agcgctccac tataccggac gccagctcga gcttgaggcg
     3061 catcgggttc tctccggccg ccagccttga cgctgcctcg gcgctgtcct cgggcgtgcc
     3121 gagcagcacg agctcccggt aggcgtccat ggcttcgtcg cgtatcgcca tgacgcgggc
     3181 gaactgctcg tccggcccgg cgtcgacccc tatgtcgtta ccgagcgact ttgacatctt
     3241 gcggccgtcg atacccggca agagcggcat cagcatcgcg cactgggcgt caaggccccg
     3301 gtcccgctgt agctccctac cggcgaggac gttgaacttc tggtcggtgc cgcctagctc
     3361 cacatctgcc ccgaccatca ccgagtcgta ggcctggagg accgggtaga gtagctcgtg
     3421 gagcccgacg cgcgctccat cgtcgtagcg ctttcggaag gtctcatggg ctaggagctg
     3481 ggccaggctg aaggatccgg cgaggcccag gagctcggcg agcgacagcg acccgtacca
     3541 ctcgctctgc cagcggagct ccgccttccg cccgtcgacc acgcggaaga tctgctcagc
     3601 gtagcgctcg gcgttcaggc gcacgcgctc cagcgacagc ggcggccgct cggcattgcg
     3661 cccggtcggg tcgccgatcg aggcggtgta gtcgccgatg atcagcacgg ccatatgccc
     3721 gaggtcctgg aactgcctga gcttgcccat gggtaccatg tgcccgatgt gcacggcgtt
     3781 accggtcggg tctatgccca gcttgacccg tagcggcctg cctgtctcga cgctacgctc
     3841 gagcttctcg agtagcgctt cccgggggac gatctcgtcg caaccccgtt cgagtagcct
     3901 gagctggcgt tcagctacct tcttcgcgtc catgtatgcc tcctcgtgga tcgtcgcgaa
     3961 tgagcgcggg tcggcctcgt cggggcgcca gccacggatg ataaaaaaac cgcggccggc
     4021 gccccctcgg ggaaccggcc gcgggctatc gcggcgtatg gactaccgct agcctcgcga
     4081 gatccctcgt gggagtcgcg tcgagtacga gcggtaataa tagatagcgt taacgttcat
     4141 tgcgtccatg gcgtcactgt agcacggcgc tagttcagcg gctaggcccg gtctaatccc
     4201 gttccgacca gtgctgcctg ggcaccgggt tgctaccctc ccagatgatg cgccggaact
     4261 ggacagggtc ggtgttgcct tccgaattga tgagcagcac ctggctgtcc tcgtcgagcc
     4321 ggagcgcctc cttgagcgcc ttcgcgccct tctcttgcat gatggtgacg agggcgccga
     4381 gggtcaccgc gccgctctcg cccgaaacga tgaacgggtc gcccttgagc ggcgtcgcgt
     4441 agatgcgcat gcccttggcc gcgacgtagt cgggcacggt cacgaaggcg tcgacggtct
     4501 ccctgagcac gtcccaggct atcgggctgg gctcgccgca ggccaggccg gccatgatgg
     4561 tgtcgagggc gccgcccacc gagtggggct tgccgtcgtc tacctcggcg ctatggaaga
     4621 ggcaggccgc gttgtccggc tcgacgacga cgcagaccgg cgcgtcgggc ccgaataggt
     4681 tgtggtagta gccggtgacc gccgcggcta gggcgccgac gccggcctgc acgaagatgt
     4741 gcgtcggctt gacgacgccc tgggcgctga actgctcctg ggcctcggcg agcatcgtcg
     4801 tatagccctg catgatccag gtaggcacgg ccgtatagcc gtcccagctg gtgtcgctga
     4861 tgatctccca gccgtacttg gcgctgtcga cggctatctg cctgaccgcg tcgtcgtagg
     4921 tgccctcgat gatcttgacg gtggcgccgt agccgcgtat cgcgtcgatc cggcgtcggc
     4981 tcgtctcggc gtgcacgtag atcacgcagg ggtggccgag cttctgggcg gcccaggcaa
     5041 tgccgcggcc gtggttgccg tcggtggcgc tcgcgaaggt tatggagccg agccggtcct
     5101 tcacctcctt ggacaggagg tactcgtacg acatcttctc gtcggggata ccgagtcgct
     5161 cctggataaa gcggtacaac gcgaatgagc cgccgagcac cttgaagctg ttgagctcca
     5221 tgcgcgacgc ctcgtccttg acccagatgc cgccgacccc gaacatggcc gacaggtgcg
     5281 gcagggcgag gagggggctc gtcctgtacc ccggtatctg gcgatggaat ttcctgctct
     5341 tcctggccat ctcgatcgga aataactcgc gggaggccga gaggtcccgc tccccgtgct
     5401 cgcggaggat ccaggcaata tccagctccg tgtctatcat cgtacgctcc tgatgtccta
     5461 ttgtcgaaat atccacggtc ttgtcaagga cggcgcggga ttgcgctagg atgcgccgtg
     5521 gcttagaatc cgggtatgaa cgaaactaag gaaaagtctc agtggcgcgg gcgcatagcc
     5581 gccgtaacga ccttcaagta cctgtccccg agcgagctcg acttcctgat cgataaggcc
     5641 gccgtggtga cctgccgccc cgaggagacc atcgtccggg aggacgaggt cagccaatac
     5701 ttcttcgcca tcctcgacgg caccgtcaac gtgacggtgg acgaggcctc tctggacggt
     5761 tcgctccgta gcgtatacat ctgcacgctc ggccccggcg acgtcttcgg agaggccggc
     5821 atcttcatca aggtcaagcg gaccgcgaca gtcagcgccg ccgacgaggt gacgctcctg
     5881 cgcctgcacc gagacgacat gagcgccttc atccggagca agccggccgg cgggaacaag
     5941 ctcctgctcg tgatcatcta cagcctgctg cgcaagctgc gcgcggccaa tcaggagctg
     6001 gcctacgagc gcaaggccga catggaccag gccgacgtcg acagcctgat ggccgacatg
     6061 ctcggcgact gacgcgagcc gggccggcta gaggcccgac agcagctccg cgatagccac
     6121 cggacggccg tccacgtcgt gcctgacgca tacctcgagg caaggcgcgg cgcagccgac
     6181 gcacgagacc cggccgggct cgcggcctgg ccgcccgcgg tcgctctccg cgacgcgcct
     6241 cgcggcgccg gcttcgttgc cgcattccag gcgccgcagg gcgcccggta cgtcgatgcc
     6301 ggcggggcag gccttggcgc agggcggctc ctcgcaccag cggcagcgcg acgcctcgac
     6361 gccggcagcg ctcgcgggga cgttgcccgg cgtcgaggcg tcgcgctg
//
LOCUS       DAOPHV010000070         6383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_15817_length_6383_cov_4.210651, whole genome shotgun sequence.
ACCESSION   DAOPHV010000070 DAOPHV010000000
VERSION     DAOPHV010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15817_length_6383_cov_4.210651"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PLQ29_03045"
     CDS             <1..762
                     /locus_tag="PLQ29_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG85652.1"
                     /translation="RIEKVTDSVIKANEYYGALYETSGVGATLLADVIQKIKLVYGQS
                     ETLLETNSIIAGIAAETNLLAMNAAIEAAHAGESGKGFAVVADEIRKLSASTSEQSKS
                     IKGMLAEVAGTIGDIAESSREAGSNFDGIRDLIRTVTRLEEEIRQAMEEQNAGSNRIL
                     SSLDSMKQASRAMDGEAEAVADIAGQISLETEALTRYSTNIQESISGVGTCAASIRQA
                     VAKAEELTTMNKASIDRMDERLGVFRLKGEADEPA"
     gene            complement(759..1748)
                     /locus_tag="PLQ29_03050"
     CDS             complement(759..1748)
                     /locus_tag="PLQ29_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HPG85653.1"
                     /translation="MRGSIATGLRALALCALGLSAVAAGAQPLAGGAEPGGVAFREVW
                     AYLMRGEERFLGAGLPITDLCLFSAEINAYGELVGVPPAASVSSFPGRKHLVVAEIGS
                     YSLTHFCLDPAFPIRAALVDAIVAAAEPFDGVQIDFEAIPVRDRDNFVTFLGQLKAGL
                     GPRILSVAVPARLSAAGDALGYARLAAVADRIVVMAYDEHWSTSEPGPVASMDWCRSV
                     AAYARSVVPVDRLVMGLPFYGRAWGDRRTNRALKYSAVEELIAEKGIGVFFRESGIPR
                     VRYQETVNVDVYYDDAESLLERMRLYRDSGVRAVAFWRLGQEDPAVWKGVGRD"
     gene            complement(1756..>3497)
                     /locus_tag="PLQ29_03055"
     CDS             complement(1756..>3497)
                     /locus_tag="PLQ29_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85654.1"
                     /translation="AAEPVPEAGAEPIAEPIAEPIVEPAAEPIAEPAAEPIAEPAAEP
                     VPEAGAEPIAEPIAEPAVEPIAEAGEAEEALMAEIMRLLAERDSLYEEINELRAENLA
                     LAQAADEAEYYRLRTGELESELERLEAELAAKEAELAAKEAELADADARLDAIGAQLA
                     EEGARLADAEARLALVELDDDALGAALIEKDALLAEKDALLSEKDASLAEKDATIAEK
                     DASLAEKDALLVERDASVAELDARIASLDSELAEARAALAEAESTLYESTERAAPVAT
                     VAPTAAPVRDDGGEASAATIAALEARVAELSAERDAAVATRDAAVATRDASDAMRLAT
                     ERILDETKAELERALADAKASGAIVAAPVPAATPGPSTVARPAPSGGYLSGWAIDSSR
                     FTRSLRSGFDGSAARMGAWKLAGQVASQTDPKQYFSRLEMPLAQGQAATLYRFKARST
                     GDGWVGVGLHLYVEDVKKRHGYGEGRSLLVWFTRDRAARGDDATYLQLYRSDDDVVME
                     RMFDAELSDGIEAWRTVEVLYDPAAEFIAVSVDGVLRIVYKTFFGRDSGATVSLRTLG
                     AGGSFSDFSAWAE"
     assembly_gap    3498..3597
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3598..3826)
                     /locus_tag="PLQ29_03060"
     CDS             complement(<3598..3826)
                     /locus_tag="PLQ29_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85655.1"
                     /translation="MRNRRSYILALGIAAFGIVALGVVWAQDSGSGGEASVQSAYDIF
                     GLSPDDTGDDAGDEAPTGDEALESLATDAVPE"
     gene            4047..4475
                     /locus_tag="PLQ29_03065"
     CDS             4047..4475
                     /locus_tag="PLQ29_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HPG85656.1"
                     /translation="MNILIANDHGAVELKTAIVAALAAKGHRVENLGVDTGDSVDYPD
                     MAKLAAERYLAGGFDFGILCCGSGIGISIAANKVDGIRCAQVFDLYTAEMCKRHNVAN
                     FIAFGGRVEYAVPVVDMIERYMATEFEGGRHRRRVDKLCR"
     gene            complement(4472..5140)
                     /locus_tag="PLQ29_03070"
     CDS             complement(4472..5140)
                     /locus_tag="PLQ29_03070"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPG85657.1"
                     /translation="MRPSNRSGPGALTDAVYARIRDAIVSAELRPNVRLVESELAVRL
                     DVSRTPVREALLRLEQEGFVRRERGWVVRENEPGEVRSRLECRVAIEGYAARLASQRR
                     DERQLASLRELASAMLEGDREPGRLHALNDAFHGLILEASGNGTLAALHAQTKMNYWD
                     LSVPVVFSAEADRLVDEQHAALLAAIADRDAGEAERVARAHVQGTLDIVLRELTARYP
                     GFSA"
     gene            5243..6193
                     /locus_tag="PLQ29_03075"
     CDS             5243..6193
                     /locus_tag="PLQ29_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85658.1"
                     /translation="MTINDFIALDWAETEGPLIEPPPFSPVVADPSFLFPEETPGGDW
                     ELFAHSAWGVRRYSSADGLSWRRRRLVVANAMRPFVRAIGGGFLLYVERYRPLALPLT
                     ALPGRRRWSSAIAVSASTDLDSWSTPRTLLEPTLDWMRDERLGESVSNPCLTNDGDEW
                     RLYFSASLAWIEDCGFCEPRTIGLAVGPSPTGPFEPTREPVIEPAPGDLAAGSIKAIR
                     VDDGWIGLQNRLYVDAEGRSRSAIFALRSEDGRSWLPAMDEPLLAPKPGWASSHVYAC
                     DCRFREADGLWYLYFNARDGWRIRDGRERIGRVVGKPREA"
     gene            complement(6248..>6383)
                     /locus_tag="PLQ29_03080"
     CDS             complement(6248..>6383)
                     /locus_tag="PLQ29_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPG85659.1"
                     /translation="AIPGFYAAGEVTGGIHAGNRLGGNAIADIMVFGRIAGASAASSK
                     "
BASE COUNT          972 a         2206 c         2143 g          962 t
ORIGIN      
        1 aggatagaga aggtgaccga cagcgtcatc aaggccaacg agtactacgg cgcgctctac
       61 gagacctccg gcgtcggggc caccctgctc gccgacgtca tccagaagat caagctggtc
      121 tacggccagt ccgagaccct cctggagacg aacagcatca tcgccggcat cgccgccgag
      181 acgaacctcc tggcgatgaa cgccgccatc gaggcggccc acgccggcga gtcgggcaag
      241 ggcttcgcgg tcgtggcgga cgagatacgc aagctgtcgg cgagcacgag cgagcagtcg
      301 aagagcatca agggaatgct cgccgaggtg gccggcacga taggcgatat tgccgagtcg
      361 tcccgcgagg cggggtcgaa cttcgacggc atacgcgacc tgatacgcac ggtcacgagg
      421 ctcgaggagg agatcaggca ggcgatggag gagcagaacg ccggctccaa ccgcatccta
      481 tcgtccctcg actcgatgaa acaggcgtcc agggcgatgg atggcgaggc cgaggccgtc
      541 gccgacatag cgggccagat atcgctcgag accgaggccc tgacccgcta ctcgacgaat
      601 atccaggaga gcatctcggg cgtcgggacc tgcgcggcca gcatccgcca ggcggtggcc
      661 aaggccgagg agctgacgac gatgaacaag gcgagcatcg accgcatgga cgagcggctc
      721 ggggtgttca ggctcaaggg cgaagcggac gagccggcct agtctcggcc cacgcccttc
      781 catacggccg ggtcctcctg gccgaggcgc cagaacgcta ccgcccggac gcccgagtcg
      841 cggtagagcc gcatccgctc gagcagggat tcggcgtcgt cgtagtacac gtcgacgttg
      901 accgtctcct ggtaccggac gcgcgggatg ccgctctcgc ggaagaacac gccgatgccc
      961 ttttccgcaa tcagctcctc gaccgccgag tacttgagcg cccggttcgt acgccggtct
     1021 ccccaggccc ggccgtagaa cggcagcccc atcacgagcc gatcgaccgg cacgacggaa
     1081 cgggcatagg cggccacgga ccggcaccag tccatggaag ccaccggccc cggctcgctc
     1141 gtgctccagt gctcgtcgta ggccatcacg acgatacggt cggcgacggc cgctagcctg
     1201 gcgtagccga gcgcgtctcc ggctgcggac agcctggccg ggaccgcgac gctcaggatc
     1261 ctggggccga ggcccgcctt gagctgcccg aggaaggtca cgaagttatc gcggtctctg
     1321 accggtatcg cctcgaagtc tatctgcacg ccgtcgaagg gctcggccgc cgcgacgatg
     1381 gcgtcgacga gggccgcccg tatcgggaag gccgggtcga ggcagaagtg cgtcagcgag
     1441 tagctgccta tctcggccac cacgaggtgt ttccgcccag ggaacgacga taccgacgcg
     1501 gccggcggca ccccgacgag ctcgccgtac gcgttgatct ccgcgctgaa taggcacagg
     1561 tcggtaatag gcaggccggc gccgaggaaa cgctcctcgc cgcgcatcag ataggcccag
     1621 acctcgcgga aggctacccc accgggctcc gctcctccgg ccaggggctg ggcgcccgcc
     1681 gcgaccgccg acaggccgag agcgcagagg gccagcgcgc ggagccccgt ggcgatcgat
     1741 cccctcacgg ccgccctact cggcccaggc ggagaagtcg gagaacgaac cgccggcgcc
     1801 gagggtccgt agcgacacgg tggcgcccga gtcacggccg aagaaggtct tgtagacgat
     1861 gcgcaggacg ccatcgacgg agacggcgat gaattcggca gcggggtcgt agagtacctc
     1921 gaccgtgcgc caagcctcga tgccgtcgga cagttccgcg tcgaacatgc gctccatcac
     1981 cacgtcgtcg tcagatcggt acagttgcag gtaggtggcg tcgtcgccgc gggccgcccg
     2041 gtcgcgcgtg aaccacacga gaaggctccg cccctcgccg tagccgtggc gcttcttcac
     2101 gtcctcgaca tagaggtgca gaccgacgcc gacccagccg tcgccggtcg agcgcgcctt
     2161 gaagcggtac agcgtggcgg cctggccctg ggccagcggc atctccagcc gggagaagta
     2221 ctgcttcggg tcggtctggc tcgcgacctg gccggctagc ttccaggccc ccatccgcgc
     2281 cgccgagccg tcgaagcccg agcgcagcga tctggtaaag cgagacgagt cgatggccca
     2341 gccggacagg tagccgccgg acggcgccgg cctcgctacg gtggacggcc caggcgtcgc
     2401 ggccgggacc ggagctgcta cgatggctcc gctcgccttg gcgtcggcca gggcccgctc
     2461 gagctcggcc ttcgtttcat cgaggatgcg ctcggtggcc aggcgcatcg cgtcggaggc
     2521 gtcgcgcgtg gcgaccgccg cgtccctcgt cgctacggcc gcgtcgcgct cggcgctcag
     2581 ctcggccacc ctcgcctcca gggcggctat cgtcgcggcc gaggcctcac cgccgtcgtc
     2641 acgaacgggg gcggccgtgg gcgctacggt tgcgacgggc gccgcccgct cggtcgactc
     2701 gtagagcgtc gactcggcct cggccagcgc ggccctcgcc tcggcgagct cggagtcgag
     2761 cgaggcgatg cgcgcgtcca gctcggcgac gctcgcgtcc cgttcgacga gcagggcgtc
     2821 cttctcggcc agcgacgcgt ctttttcagc tatcgtcgcg tccttctccg cgagcgaggc
     2881 gtccttctcg gatagcaacg cgtctttctc ggccagtagc gcgtccttct cgatcagcgc
     2941 ggcaccgagc gcgtcgtcgt ccagctcgac gagggccagg cgggcctcgg cgtcggccaa
     3001 cctggcgccc tcttcggcta attgggcccc gatggcgtcc aatcgggcgt ccgcatcggc
     3061 caattcggct tccttggcgg ccagctcggc ttccttggcg gccagctcgg cttcaagccg
     3121 ctctagctcc gactccagct ctcccgtgcg cagtctatag tactcggcct cgtcggccgc
     3181 ctgggcgagc gctaggtttt cggcgcgtag ctcgttgatc tcctcgtaga gggagtcgcg
     3241 ctcggcgagt agccgcatga tctccgccat gagcgcttcc tcggcctcgc cggcttcggc
     3301 tatcggctcc acggccggtt cggctatcgg ctcggctatc ggctcggccc ccgcctcggg
     3361 tacaggctcc gcggccggct cggctatcgg ctccgcggcc ggctcggcta tcggctccgc
     3421 ggccggctcg actatcggct ccgctatcgg ctcggctatc ggctcggccc ccgcctcggg
     3481 tacaggctcc gcggccgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngct
     3601 ccggcacggc gtccgtggcc agcgactcga gagcctcgtc ccccgtaggg gcctcgtcgc
     3661 cggcatcatc gcccgtatcg tcggggctca ggccgaagat atcgtaggcg ctctggacgg
     3721 aagcctctcc gccagaacct gagtcctgag cccacacgac cccgagggcg acgatgccga
     3781 aggcggcgat gcccagggcg aggatatacg aacgacgatt gcgcatgagt cggctcctct
     3841 cgggacgggc ggaccctcgc gggatgcccc tgcttcagta tcggcctgga acgccccgtc
     3901 gtttagccgc tatcctaggt tccgggaccg ctacctcggg gaccgcttca tcgggtaccg
     3961 cctgcggata atcgacgaga tacggactga agggggcatc gcccagatcg agcgtccatg
     4021 gtaggatggc gggcggagga cggaacatga acatcctgat agccaacgac cacggcgcgg
     4081 tcgagctcaa gacggccatc gtcgcagccc tcgccgccaa ggggcaccgg gtcgagaacc
     4141 tcggcgtcga cacgggtgac tcggtggatt atccggacat ggcgaagctg gcggccgagc
     4201 gatacctggc cggcggcttc gacttcggca tcctgtgctg cggctccggc attggcatct
     4261 cgatagccgc gaacaaggtc gacggcatac gctgcgccca ggtcttcgac ctgtacaccg
     4321 ccgagatgtg caagcgccac aacgtcgcca acttcatcgc gttcggcggc cgagtcgagt
     4381 acgccgtgcc ggtcgtcgac atgatagagc gctacatggc taccgagttc gagggcggac
     4441 gccaccggag gcgcgtcgac aagctgtgcc gctaggccga gaagcccggg tacctcgccg
     4501 tgagctcgcg gagcacgatg tcgagggttc cctgtacgtg ggccctggcc acgcgctccg
     4561 cctcgccggc gtcgcggtcg gctatggcag cgagcagggc cgcgtgctgc tcgtcgacca
     4621 gcctgtcggc ttcggcggag aatacgaccg gcacgctgag gtcccagtaa ttcatcttgg
     4681 tctgcgcgtg cagcgcggcc agcgtcccgt tgcccgacgc ctcgagtatc agcccatgga
     4741 aggcgtcgtt cagcgcgtgg agcctgcccg gctcgcggtc gccttcgagc atcgcggagg
     4801 ccagctcgcg cagcgacgcg agctgcctct cgtcacgccg ctggctggcc agccgggccg
     4861 cgtagccctc gatggccacg cggcactcga gccttgagcg cacttccccg ggctcgttct
     4921 cgcgtaccac ccagccccgc tcgcgcctca cgaagccttc ctgctccagc cgcaggaggg
     4981 cctcgcgcac cggcgtccgc gagacgtcga gcctgacggc cagctcgctc tcgacgaggc
     5041 ggacgttcgg cctgagctcg gcgctcacga tggcgtctcg gatgcgggcg tagaccgcgt
     5101 cggtcaaggc cccggggccg gatcggttcg atggtcgcat ggtcacacag tataccaacc
     5161 gcggcccagc cgcgctacgg cgtcaaggcg gcgtcgtcgg gcggagcttg cgctcgccta
     5221 tgggcggcgc tatgctttcg ggatgaccat aaacgatttc atagcgttgg actgggccga
     5281 gaccgagggc ccgctcatcg aaccgccgcc cttctcgccg gtcgtcgccg acccgagctt
     5341 cctgttcccc gaggagaccc cgggcggcga ctgggaactg ttcgcccact cggcctgggg
     5401 cgtgcgccgc tactcgtcag ccgacgggct atcgtggcgc aggcgccgcc tcgtcgtggc
     5461 taacgcgatg cggcccttcg tccgcgccat cggcggcggg tttctcctct acgtcgagcg
     5521 ataccgtccg ctcgccctgc ccctgactgc cttgcccggc cggcggcggt ggagttcggc
     5581 catagcggtt tcggcctcta ccgacctcga ctcctggtcg acgcccagaa ccctgctcga
     5641 gcccaccctg gactggatgc gcgacgaacg gctcggcgag tctgtgtcga acccctgcct
     5701 gacgaacgac ggagacgagt ggcgcctgta cttctccgcg tcgctggcct ggatagagga
     5761 ctgcggcttc tgcgagccca ggaccatagg cctggccgtc gggccgtcgc cgaccggacc
     5821 cttcgagccg acgcgggagc cggtcatcga gccggccccc ggggacctcg ccgcgggctc
     5881 gatcaaggcc atacgggtgg acgacggctg gatagggctt cagaaccgcc tctacgttga
     5941 cgccgaaggc cgctcgcgct cggccatctt cgcgctgcgc tccgaagacg gacgctcctg
     6001 gctgccggcg atggacgagc cgctcctggc gccgaagccg ggctgggcct cgtcccacgt
     6061 ctacgcctgc gactgtcgtt tccgcgaggc cgacggactg tggtacctgt acttcaacgc
     6121 gcgcgacggc tggaggatac gcgacggccg agagcgcata ggccgcgtcg tcggcaaacc
     6181 gcgcgaggcc taaaaaaacg ccggccggga agcgttcccg gccggcttcg gtcatatagc
     6241 cagacgatta cttcgacgaa gcggcgctgg cgccggctat ccggccgaag accatgatgt
     6301 cggcgatggc gttgccgccg aggcggttac cggcgtggat accgccggtg acctcgccgg
     6361 cggcgtagaa gccggggatg gcc
//
LOCUS       DAOPHV010000071         6345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_15956_length_6345_cov_3.790938, whole genome shotgun sequence.
ACCESSION   DAOPHV010000071 DAOPHV010000000
VERSION     DAOPHV010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15956_length_6345_cov_3.790938"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLQ29_03085"
     CDS             complement(<1..283)
                     /locus_tag="PLQ29_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85660.1"
                     /translation="MTLLTLNLHKPLAWSTAQASQVGVNQASDAVLNAVSFPEGAELA
                     LSWAWETVVDGRGDDGPRTIRPLAAPESVAGSGLGDAAAALDVSLKTGRY"
     gene            356..2338
                     /gene="dxs"
                     /locus_tag="PLQ29_03090"
     CDS             356..2338
                     /gene="dxs"
                     /locus_tag="PLQ29_03090"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPG85661.1"
                     /translation="MSILERISGPSDLRGLSVAELHRLSGELRSLIVATVTANGGHLA
                     SNLGVVDLTLALHCVFSTPEDAIVWDVGHQCYAHKILTGRRDRFARLRRSGGLSGFPK
                     RSESRHDAFGAGHASTSISAALGLLAANQRLGSAGSVVAVIGDGALTGGMAYEAMSHA
                     GQLGLPLIIVLNDNAMSISRNVGAVSSYLSRLSATTRYQRLRRLIDRVILGVPWFGPR
                     VHPLVLRLKRAVKAVFFKENLFADLGFEYAGPIDGHNIQLLREVLGEARAIHKPVVVH
                     VITKKGKGHVAAEANPSAFHGVSPASSPAMQSSPASSPASSPASSPAMQSSDPLDPPD
                     QPAAKRPTFTEAFGQALLAAASGDDRVVAVTAAMSSGTGLDGFQRAFPERCFDVGIAE
                     EHAVTFAAGMAAGGLRPVVAIYATFMQRAFDQVHHDVALQGLPVVFALDRAGAVPDDG
                     ETHQGIYDIQAYRAVPGLSILAPASSGEVGQALRWALAHDGPTMIRYPKAACPPERPA
                     YAEPFVAGRGVCVRKAEAGAEALFVASGALVDEALAAADACAASGLAADVYHLRFLKP
                     VDSSAFAAMAARYRCVVIAEEGVLTGSVASDLAAAAAGDGRAVVALGFDEKPLAQARR
                     DELLAAAGLSADALAATAIALARPTDSGALAIGTGS"
     gene            2341..3231
                     /gene="folP"
                     /locus_tag="PLQ29_03095"
     CDS             2341..3231
                     /gene="folP"
                     /locus_tag="PLQ29_03095"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPG85662.1"
                     /translation="MAVTGVGGHRSLRLPRGRSLLLSGPAVVMGIINATPDSFYAASR
                     SADVGKAVDQAAAMIGAGAAVLDVGGESTRPGAAYVGADEESARVAPVIAALRARWDI
                     PISVDTRKASVARAALDAGADIVNDVAALLDDPGLAPLCAERGAPVVLMHKKGVPESM
                     QDGPWYDDCVSEVKAFLLAAADAAIASGVSRDAILLDPGVGFGKRLDDNLALMARLDE
                     IAASGYPVLVGLSRKSFIGALSGGGAEDRLPGSLAAACVARARGAVVFRVHDVAETVQ
                     ALAVLDAAMRGWASVRATLE"
     gene            3259..4059
                     /gene="cdaA"
                     /locus_tag="PLQ29_03100"
     CDS             3259..4059
                     /gene="cdaA"
                     /locus_tag="PLQ29_03100"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HPG85663.1"
                     /translation="MGIQAFFNAFTRWARPALDIAILAYLLYAMYRLLIKTQAVQLAK
                     GAALLVFVYAGAFFLKLETLSWVLNLLAPGLVIAMAIIFQPELRKIFIKLGQGGIFKR
                     GQGPRSTQLDAILHAAELLAEKRRGALIAFVRFVALDDIVERGTRIDGEVSSALILSI
                     FEYDTPLHDGALIVKEGRILAAGCFLPLSEQRDIKRSFGTRHRAALGLTESADAVVLI
                     VSEETGAISLAYDARLYYNLGLPALRKRLGQLLESSGKPLGEEAAFDE"
     gene            4052..5023
                     /locus_tag="PLQ29_03105"
     CDS             4052..5023
                     /locus_tag="PLQ29_03105"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HPG85664.1"
                     /translation="MSKSRMELLERLLADWPAKVLSLAGALALSYFYQVNKLEERPMS
                     VPLVVVTNDDFVPASQYPRTVRLVLRGESNAIYAVLEGDIEATLDLRGYTGAGVWRVP
                     VRVEKRGSALGIDPLEISVDPSDVALSIEPRVVKEVPVVPSFRGYLEPGFELAGFRLT
                     PARMEIAGPSSAMEKLADAATDPIELTGRSDNFELRTKVQLRDPLLVLLSSNLVDFSA
                     EVKKALVYRNFQGLELSYVGLRDGLALLEFGTMGAARVAASSVELEAFATGPGLLEAD
                     LSNIEKPGVYTVAVSPRFPAGFDVETWSPMVQTVTVVDSDDAPEVLP"
     gene            5020..5391
                     /locus_tag="PLQ29_03110"
     CDS             5020..5391
                     /locus_tag="PLQ29_03110"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPG85665.1"
                     /translation="MILGVGVDVVHVRRIIHWLSVPGLPERFFHPDELSSARSRGSSV
                     ALSLAARFAAKEAFGKAIGTGLRGIRLADICVINNHNGRPDMRLQGSALAKYEALGCS
                     AVHLSLTHESDNAIAMVVLEA"
     gene            5393..6274
                     /locus_tag="PLQ29_03115"
     CDS             5393..6274
                     /locus_tag="PLQ29_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HPG85666.1"
                     /translation="MQKILTSTKQTKDRKITFYAKEPTRCPVCDASFHREELFSGRVN
                     ADEMTDELHRTYKPLSAFGEVYPLVYEIEVCPACFYAAYRPDFMAAGAKFADALRERT
                     DARVEEAQRVFSPIDYQSPRNLLCGAASYYLAMQCYELFSKEFAPTIKCAISSIRTAW
                     LCHDLHAKAPTENWDYVAGLFYRKARFYYRAAIELEQSGKEPYSSQRNLGPDTDKNYG
                     HEGVLYTAAILELKYGPTDDPAIREERLKAAKVSVARMFGFGKKNKSKPGPLLEKARD
                     LYERLKRELREGDDEDE"
BASE COUNT          946 a         2215 c         2162 g         1022 t
ORIGIN      
        1 ggtagcggcc ggtcttcaga ctgacgtcca aagccgccgc agcgtctccg agaccggagc
       61 cggcgacgct ctcgggggcg gcgagggggc ggatggttcg ggggccatcg tcgccgcgcc
      121 cgtcgacgac ggtttcccaa gcccaggaga gggccagctc ggcgccctcg gggaacgaga
      181 cggcgttcaa gacggcgtcg gacgcctggt tgacgcccac ctgggaggcc tgggcggtcg
      241 accaggcgag cggtttatgg aggttcagcg ttaggagagt catggtggca gtatggtggc
      301 ttttaggggg gcgggtatag tcccctcgac cgctcgcatg gtacactcga gcgtcatgtc
      361 gatccttgaa cgcatatccg gtccgtccga cctgaggggc ctgtcggtcg ccgagctcca
      421 tcgcctgtcg ggggagctcc ggtccctgat cgtagccacc gtcaccgcga acggcggcca
      481 cctggcctcg aacctgggcg tcgtcgacct gacgttggcc ctgcattgcg tcttctcgac
      541 gcccgaggac gccatcgtct gggacgtggg gcaccagtgc tacgcccata agatactcac
      601 cggccggcgc gaccggttcg ccaggcttcg gcgctcgggg gggctgtcgg gctttcccaa
      661 gcggtccgag agccgccatg acgcgttcgg cgccgggcac gcctcgacct cgatctcggc
      721 cgcgctcggc ctgttggccg cgaaccagcg gctcggctcc gcgggttcgg tggtggcggt
      781 gataggcgac ggcgcgctga ccggcggcat ggcctacgag gcgatgagcc acgccggaca
      841 gctcggcctg cccctgatca tcgtgctcaa cgacaacgcg atgtccatct cgaggaacgt
      901 cggcgcggtc tcgagctacc tgtcgcgcct gtcggcgacg acccgctacc agcggctcag
      961 gcgcctcatc gaccgcgtca tactcggcgt cccgtggttc ggcccgcgcg tccacccgct
     1021 cgtgctgagg ctcaagcgcg cggtcaaggc cgtcttcttc aaggagaacc tgttcgccga
     1081 cctcggcttc gagtacgccg ggccgatcga cggccacaat atccagctcc tgcgagaggt
     1141 gctcggcgaa gcccgggcca tccacaagcc cgtggtcgtc catgtcatca cgaagaaggg
     1201 caagggccat gtcgccgcgg aagccaatcc atccgccttc catggcgtgt cgcctgcgtc
     1261 gtcgcctgct atgcagtcgt cgcctgcgtc gtcgcctgcg tcgtcgcctg cgtcgtcgcc
     1321 cgctatgcag tcgtccgacc cgctcgaccc gcccgaccag ccggcggcca agcggccgac
     1381 cttcaccgag gccttcgggc aggccctgct cgcggcggcc tccggcgacg atagggtcgt
     1441 ggcggtgacg gccgcgatgt cctcgggtac cggcctcgac ggcttccaac gggccttccc
     1501 cgagcgttgc ttcgacgtgg gcatagccga ggagcacgcg gtcaccttcg cggccggcat
     1561 ggccgcgggc ggcctcaggc cggtcgtcgc catctacgcc accttcatgc agcgcgcctt
     1621 cgaccaggtg caccacgacg tggcgctcca gggcctgccc gtggtgttcg cgctcgaccg
     1681 tgccggcgcc gtgccggacg acggcgagac gcaccagggc atctacgaca tccaggccta
     1741 ccgggccgtg cccggcctgt cgatactggc cccggccagc tcgggggagg tcgggcaggc
     1801 cctccgctgg gccctggccc acgacgggcc gacgatgatc cgctacccga aggccgcctg
     1861 ccccccggag cggccggcct atgccgagcc gttcgttgcc gggcgcggcg tctgcgtgcg
     1921 caaggccgag gccggggcgg aggcgctgtt cgtagcctcg ggagccctgg tcgacgaggc
     1981 gctcgccgcg gccgacgcct gcgccgcgtc cggcctggcc gccgatgtct accatctgcg
     2041 cttcctcaag ccggtcgata gctcggcctt cgccgcgatg gcggcccgct accgctgcgt
     2101 ggtgatagcc gaggagggcg tgctgaccgg aagcgtcgcc tccgacctgg ccgccgccgc
     2161 cgccggggac ggccgggccg tcgtcgccct cggtttcgac gagaagccgc tggcgcaggc
     2221 ccgccgcgac gagctcctgg ccgccgcggg actatcggcc gacgcgctgg ccgctacggc
     2281 catcgcgctg gccaggccga ccgactccgg ggccctggcc ataggtacgg ggagctgatc
     2341 gtggccgtga ccggtgtcgg cggccatcgc tcgctcaggc taccgcgcgg gcggtcgctc
     2401 ctcttgtccg gccccgccgt cgtgatgggc atcatcaacg cgacgccgga ctcgttctac
     2461 gcggctagcc gctcggccga cgtcggcaag gccgtcgacc aggcggccgc gatgataggc
     2521 gccggggccg ccgtcctgga cgtcggcggc gagagcacgc ggccaggcgc cgcgtacgtc
     2581 ggcgcggacg aggagtcggc ccgggtagcc ccggtgatag ccgcgctacg cgcccgctgg
     2641 gacataccga tctcggtgga cacgcgcaag gcctcggtgg cccgggcggc cctcgacgcc
     2701 ggcgccgaca tcgtcaacga cgtggccgcc ctgctcgacg acccggggct ggccccgctc
     2761 tgcgccgagc gcggcgcccc ggtcgtcttg atgcacaaga agggcgtgcc cgagagtatg
     2821 caggacggtc cctggtacga cgactgcgtc tccgaggtca aggccttcct gctcgcggcc
     2881 gccgacgctg cgatcgcgtc cggggtctcg cgcgacgcca tcctcctcga ccctggcgtc
     2941 ggcttcggca aacggctcga cgataatctc gccctcatgg ctcggcttga tgaaatagcc
     3001 gcctcgggct atcctgtcct cgtcggcctg tcgcggaagt cgttcatagg ggcgctcagc
     3061 gggggcgggg ccgaggatag gctcccgggt agcctggcgg ccgcctgcgt cgccagggcg
     3121 cgcggggcag tcgtcttcag ggtgcatgac gtggccgaga cagtccaggc tctggccgtc
     3181 ttagacgcgg ccatgcgcgg gtgggcctcg gtacgggcca ctctggaata gaacgacgga
     3241 aggcggacgg acgggacgat gggcatacag gcgttcttca acgcgtttac tcgatgggcc
     3301 cgtccggccc tcgacatagc catactcgcg tacctcctgt acgccatgta ccgcctgctc
     3361 atcaagacgc aggcggtgca gctggccaag ggcgcggccc tgctcgtgtt cgtctacgcc
     3421 ggcgccttct tcctgaagct cgagaccctg tcctgggtgc tcaacctgct ggcccccggc
     3481 ctcgtcatag cgatggcgat catcttccag ccggagctgc gcaagatctt catcaagctc
     3541 ggccagggcg gcatcttcaa gcggggccag gggccgcgct cgacccagct cgacgccata
     3601 ctgcacgccg cggagctact cgccgagaag cgccgcgggg cgctgatagc cttcgtgcgc
     3661 ttcgtggccc tcgacgacat agtcgaacgc ggcaccagga tcgacggcga ggtgagctcg
     3721 gccctgatcc tatcgatctt cgagtacgac acgccgctcc acgacggcgc cctgatcgtc
     3781 aaggaaggtc gcatcctggc ggccggctgc ttcctgcccc tgtccgaaca gcgcgacatc
     3841 aagcggagct tcggtacgcg ccaccgggcc gccctcgggc tgaccgagag cgccgacgcc
     3901 gtcgtgctga tcgtatccga ggaaaccggc gccatctcgc tggcctacga cgccaggctg
     3961 tactacaacc tgggccttcc cgcgctacgc aagcggctcg gccagctgct cgagtcgtcg
     4021 ggtaaaccgc tcggcgagga ggccgccttc gatgagtaaa tcgaggatgg agctgctcga
     4081 gcggctattg gccgactggc cggccaaggt cctatcgctc gcgggcgccc tggccctgtc
     4141 gtacttctac caggtcaaca agctcgagga gcggcccatg tcggtgcccc tcgtcgtcgt
     4201 gaccaacgac gattttgtgc cggccagcca gtacccgcgc acggtgcgcc tcgtcctgcg
     4261 aggcgagtcg aacgctatct acgcggtgct cgagggcgac atcgaggcga ccctcgacct
     4321 gcgcggctac acgggcgctg gcgtctggcg ggtgccggtc cgcgtcgaga agcgcggcag
     4381 cgccctcggg atagacccgc tcgagatcag cgtcgacccg tcggacgtcg cgttgtcgat
     4441 cgagccgagg gtggtcaagg aggtaccggt ggtgccgagc ttccgtggct acctggaacc
     4501 gggcttcgag ctggcgggct ttcggctgac gccagccagg atggagatag ccgggccgtc
     4561 gagcgcgatg gagaagctgg cggacgccgc caccgatccc atcgagctga ccggccgctc
     4621 ggacaatttc gagttgcgca ccaaggtgca gttgcgcgac ccgctcctgg tcctcctgtc
     4681 gtccaacctc gtcgacttca gcgccgaggt caagaaggcg ctcgtgtacc gcaatttcca
     4741 gggactcgag ctgtcctatg tcgggttacg cgacgggctg gccctgctcg agttcgggac
     4801 gatgggggcc gcgagggtcg ccgcctcgag cgtcgagctc gaggccttcg cgaccggccc
     4861 cggcctgctc gaggccgatc tgtcgaacat cgagaagccc ggcgtctata cggtcgccgt
     4921 ctcgccgcgc ttcccggccg gcttcgacgt cgagacctgg tcgcccatgg tccagaccgt
     4981 caccgtcgtc gactcggacg atgcgccgga ggtgctaccg tgatactcgg cgtcggtgtc
     5041 gacgtggtgc atgtcaggcg tattatccat tggttatcgg taccgggtct gcccgagcgt
     5101 ttcttccatc cggacgagct gtcgtcggcc cggtcgcgcg gctcgagcgt ggccctatcc
     5161 ctggctgctc gcttcgcggc caaggaggcc ttcggcaagg ccataggcac ggggctgcgc
     5221 gggatcaggc tggccgacat ctgcgtgatc aacaaccata acggccgacc ggacatgcgg
     5281 ctccaaggta gcgccctggc caagtacgag gccctcggct gctctgccgt acacctttcc
     5341 ctgacccacg agagcgacaa cgccatcgcg atggtagtgc tggaggccta gcgtgcagaa
     5401 gatcctgacc tcgaccaagc agacgaagga ccgtaagatc accttctatg ccaaggagcc
     5461 gacgcgctgc cccgtctgcg acgctagctt ccaccgcgag gagctgttct ccggccgcgt
     5521 caacgccgac gagatgaccg acgagctgca ccggacctac aagcccctgt cggccttcgg
     5581 cgaggtgtac ccgctcgtct acgagatcga ggtctgcccg gcctgcttct acgcggccta
     5641 caggccagac ttcatggcgg ccggcgccaa attcgccgat gcgctacgcg agcgcaccga
     5701 cgccagggtg gaggaggccc agcgcgtctt ctcgccgatc gactaccagt cgccgcgcaa
     5761 cctcctctgc ggggcggcca gctactacct ggcgatgcag tgctacgagc tattctccaa
     5821 ggagttcgcg ccgaccatca agtgcgccat atcgtcgata cggacggcct ggctatgcca
     5881 cgacctgcac gccaaggccc cgaccgagaa ctgggactac gtcgccggcc tcttctaccg
     5941 caaggccagg ttctactacc gggccgcgat cgagctcgag cagtcgggca aggagccgta
     6001 ctcgtcgcag cgcaacctcg ggccggacac cgacaagaac tacggccacg agggcgtgct
     6061 ctataccgcg gccatcctcg agctcaagta cggtccgacc gatgatccgg ctatacgcga
     6121 ggagcggctc aaggccgcca aggtctcggt cgccaggatg ttcggcttcg gcaagaagaa
     6181 caagtcgaag ccgggcccgc tcttggagaa ggctcgcgac ctgtacgagc ggctcaagcg
     6241 cgagctcagg gagggtgacg acgaggatga gtgaccgcgt cgtcaagctg accgtggcct
     6301 acgacggcac ggatttctgc gggtggcaac ggcaggccgg cgcgc
//
LOCUS       DAOPHV010000072         6336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_15988_length_6336_cov_3.654036, whole genome shotgun sequence.
ACCESSION   DAOPHV010000072 DAOPHV010000000
VERSION     DAOPHV010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15988_length_6336_cov_3.654036"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLQ29_03120"
     CDS             <1..374
                     /locus_tag="PLQ29_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85667.1"
                     /translation="GADQLADAYLGYRYGPDAMAWSGTIGWDAPGRLRGKASLEYALK
                     GEQGMDTAYATGQEAAALRTPTGTPERIASVGIEAAWKVRPWLELGCGLVWTSVGDYG
                     HVAGATAAALDARVSIAVSYR"
     gene            401..1585
                     /locus_tag="PLQ29_03125"
     CDS             401..1585
                     /locus_tag="PLQ29_03125"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG85668.1"
                     /translation="MTRIEDRERVLFLYLPTGGGHLSNCKAISRRVAERYGAETLAYD
                     PVSARSPLGKLFLEGGYRFMSLRLPWMWNLLFELNDNPFIMRLSQGSARRFSRYGLRY
                     IIDEWKPTRIVCLHHLLSPAVAAALEGREPIPVTVVATDPFVPPKLWAHGHEYPIVCM
                     SEEARDSFLAMGVDESRLRTYSIIIGEKFERRMDEEARAAFKRSVGLDPERPLVLIAG
                     GGDGMRGADRILDELCSSSLDYQVALVCGRDERTRKRAEAKALEYRIRGRRVVVFGFT
                     DRMYELMESADAIVSKAGPAVLGEVLAAGKPNVIAFYIPGQEKPNVRWVEEKGVGVYC
                     PEPARMRAVVERLLTDDEYRAGMEDAIGRLGFKNGLYEIAEAVMSTEALAPAAPAGQE
                     AL"
     gene            1819..2097
                     /locus_tag="PLQ29_03130"
     CDS             1819..2097
                     /locus_tag="PLQ29_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPG85669.1"
                     /translation="MAKKLYVGNLSYGTSEDGLRAAFSQYGEVQSVNIVIDRDSGRSK
                     GFGFVEMPAEDAAAAAISGMNGRELDGRSLRVNEANERPPRRDYGNRW"
     gene            2226..3608
                     /locus_tag="PLQ29_03135"
     CDS             2226..3608
                     /locus_tag="PLQ29_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG85670.1"
                     /translation="MDLLPVVIVVIFLALVVIVGGAVLSKGKGGKSKGGKRIKAKDHA
                     QVLKEANRRLAQNPKDPDALQALGEVYYGEQAWDKAFKAYEVLVEVSAGNPDLDEFEI
                     NKRYGLCAMKLGRLDESYKGLVLAHAVKADDFETNYNLGYLEFQKRQYEKAIFHLRLA
                     GRQNPEHAMTLRYLGHSLFKVKNFKEALSSLKRAVDLQPDDKESLFAMGECFHEIGQE
                     EQAIRLFTHLRTDPALGPSAALFAGTIHLNQRQYQKAIMDFELGLRHPDIKVEVLVEI
                     KYRLAAAYLKGQEIAKAVSLLNEIQQIYPNYKDTPAQLAKYKELNSNRNLQVYLLSAT
                     SDFITLCRKITLTFFPKAKVKITDISVQKNDWADILAEVETSRWSDVVLFRMIRSTGT
                     IGELVLRDFHARIKDLKAGKGYCITAGVFSDESKKFVEARLIDLMEKDKLMTLLNNID
                     SQQKGLLITD"
     gene            3689..5098
                     /locus_tag="PLQ29_03140"
     CDS             3689..5098
                     /locus_tag="PLQ29_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HPG85671.1"
                     /translation="MFEPSVYSGRRRALVAALASRGALDGLVAFPGNNDSPVNYPDNA
                     YRFRQDSCFLYYFGLAEPGLAAAIDLGTGRATLYADALTIDDLVWTGPRPGVDEYRAL
                     CGADAVKPRSAFGTDLAAARGRCLFTPPYRADAAIELGEALGIAPGALSAAASNALIT
                     SVVAQREIKEDRELADIERAVDVTIDMHRAVLAAARPGVTEAAMMAEAYRVAYAGGGM
                     PAFPAIATTKGSVLHNHGYPGTLTSGGLFLLDAGAETAEGYAGDLTSTFPIGGRYDDR
                     QRAVYDIVLRAGEACSSMMAPGVPYRDCHLAAARAIAEGLSGLGVMRGDVDEAVAAGA
                     HACFFPHGVGHQMGLDVHDMENLGEVNVGYDGQPKSAEFGLKSLRLAKPLKAGLVVTV
                     EPGVYFIEGLIAAWKAEKRHASFINYAEAEQWLPVGGIRNEEDWVVVDGKGGGVGSGA
                     RRLGKPFDKSAAAMEGYRA"
     gene            complement(5112..6302)
                     /locus_tag="PLQ29_03145"
     CDS             complement(5112..6302)
                     /locus_tag="PLQ29_03145"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPG85672.1"
                     /translation="MKDSFLELSHHQVSKRGQSAPGDAFQSIKCDDGRVISVLSDGLG
                     SGIKAGVLATLTATMAARCVAADIPLRRVAEFVMRSLPVCSERRISYATFTIVDVAPG
                     GSVRVVEYDNPPYLLLRRGQALLPEKTELRLPRGRGGAKGDAVLKLSSFDAEPGDRVV
                     LYSDGVTQAGMGTRSYPLGWGDEAVKRFATAAVAEAPDGVSARELARTIVHEATAKDA
                     WAPKDDITCGVAYWRHPRRLLVLTGPPVSKDRDREMTRIFASFDGRKAIAGGTTANII
                     ARESGLAIRVDLSNLDPEIPPSSTMPGADLVTEGILTLARVAALLEAGQEEAPGRRNA
                     ATRLLELMLDSDVIQFVVGTRINEAHQDPTMPLELEIRRNVVKRIAAILESRHLKDTR
                     VSFI"
BASE COUNT         1092 a         1999 c         2197 g         1048 t
ORIGIN      
        1 gcggcgcgga ccagctggcc gacgcgtacc taggctatcg ctacgggccg gacgccatgg
       61 cctggtcggg taccattggc tgggacgcgc ctggtaggct acgcgggaaa gcgagcctcg
      121 agtacgcgct caagggcgag cagggcatgg acacggccta cgccacgggc caggaggcgg
      181 cggccctgag gacgccgacc ggcacgcctg agcggatagc gtcggtaggg atagaggcgg
      241 catggaaggt caggccgtgg ctggagctcg gttgcggcct ggtttggacg agcgtgggcg
      301 actacgggca cgtggccggc gcgacggccg cggcgttgga cgcgcgggtg tcgatagcgg
      361 tatcgtatcg ctaggcgcgg gagccgcgcc aaggaagacc atgaccagga tagaagatag
      421 ggagcgcgtg ctgttcctgt acctgcccac cggcggcggg cacttgtcca actgcaaggc
      481 gataagccgc cgcgtggccg agcgctacgg ggccgagacg ctggcctacg atccggtctc
      541 ggcgcgttcg ccgctcggca agctgttcct ggagggcggg taccgtttta tgtccctgcg
      601 cctgccctgg atgtggaacc tcttgttcga gctgaacgat aatcccttca tcatgcggct
      661 gtcgcagggc agcgccaggc gcttctcgcg ctacggtctg cgctacatca tcgacgagtg
      721 gaagccgacg cgcatcgtct gcctgcacca cctcctgtcc ccggcggtgg ccgccgcgct
      781 cgaggggcgg gagccgatac cggtgacggt ggtggccacc gaccccttcg tgccgcccaa
      841 gctctgggcc cacggccacg agtaccccat cgtctgcatg tccgaagagg ctcgcgatag
      901 cttcctggcg atgggcgtgg acgagtcgcg tctgcggacc tattcgatca tcatcgggga
      961 gaagttcgag cggcggatgg acgaggaggc gcgcgccgcc ttcaagcgct cggtagggtt
     1021 ggacccggag cggccgctgg tgctgatagc cggcggaggc gacggcatgc gcggcgcgga
     1081 ccgcatcctg gacgagctgt gctcctccag cctggactac caggtggcgc tggtctgcgg
     1141 ccgcgacgag cgtacgcgca agcgggcgga ggccaaggcg ctcgagtacc ggatccgggg
     1201 ccgccgggtc gtggtgttcg gctttaccga ccgcatgtac gagctcatgg agtcggccga
     1261 cgcgatcgtg tcgaaggccg gccccgcggt gctcggcgag gtgctggctg cgggcaagcc
     1321 gaacgtcata gccttctata tacccggcca ggagaagccg aacgtgcgct gggtcgagga
     1381 gaagggcgtg ggcgtctact gcccggagcc ggccaggatg cgagccgtgg tggagcgcct
     1441 cctgaccgac gacgagtacc gcgcgggcat ggaagacgct atcgggcggc tgggcttcaa
     1501 gaacgggctg tacgagatag ccgaggcggt gatgtccacg gaggctctcg cgccagcggc
     1561 tccggctggc caggaagctt tgtaaaaaag cctttgacaa ggtttatgtt ttgctatata
     1621 tttcgtactg ttggaaacga atcgagtttt agaggaccgt gaatctgtta gattttagcc
     1681 gagttaataa cgtgcctagc tccgcatttc ccccggcgaa tcaagcttcc atgcctagct
     1741 caatttctgg cctcctttcc cgtatccgtt ccacgactat ctcaatcccc ggcaaagatc
     1801 cgggaaaagg accttctcat ggctaagaaa ctctacgtcg gcaacctcag ctacggcacc
     1861 tccgaggacg gcctccgcgc cgcgttctcc cagtacggtg aagtgcagtc cgtcaacatc
     1921 gtcatcgacc gcgattccgg ccgctccaag ggcttcggtt tcgtcgagat gcccgccgag
     1981 gacgccgccg ccgccgccat cagcggtatg aacggccgcg agctcgatgg ccgctccctc
     2041 cgcgtcaacg aagccaacga gcgcccgccc cgccgcgact acggcaaccg ctggtaattc
     2101 gcgcatgggt cgataacgac ccgctgtagt acggattatc cacccctccc ggtctccggg
     2161 aggggttttt tatgccccga ggggtttctt gacaattgct taacctgccg cccatacttg
     2221 gggccatgga tctgttgccg gtcgtcatcg tcgtgatctt cctcgccctg gtcgtcatcg
     2281 tgggcggagc cgtcctgtcc aagggcaagg gaggcaagtc caagggtggc aaaaggatca
     2341 aggccaagga ccacgcgcag gtcctgaaag aggcgaaccg caggctggcc cagaacccca
     2401 aggacccgga tgcgctacag gcgctcggcg aggtgtacta cggcgagcag gcctgggata
     2461 aggccttcaa ggcctacgag gtcctggtcg aggtgtccgc cggcaatccc gatctggacg
     2521 agttcgaaat caacaagcgc tacggcctct gcgcgatgaa gctcggccgc ctcgacgagt
     2581 cctacaaggg cctggtgctg gcgcacgcgg tcaaggccga cgacttcgag acgaactaca
     2641 acctcggcta cctcgagttc cagaagcggc agtacgagaa ggcgatcttc cacctccggc
     2701 tggcggggcg gcagaacccc gagcacgcga tgacgctgcg ctacctgggg cattcgctgt
     2761 tcaaggtcaa gaacttcaag gaggccctat ccagcctgaa gcgcgcggtg gacctgcagc
     2821 cggacgacaa ggaaagcctg ttcgccatgg gcgagtgctt ccacgagata gggcaggagg
     2881 agcaggcgat caggcttttc acgcacctgc gcaccgaccc cgccctcgga ccgtcggcgg
     2941 cgctgttcgc cggcactatc cacctgaacc agcggcagta ccagaaggcc attatggact
     3001 tcgagctggg gcttcggcac ccggacatca aggtcgaggt cctcgtcgag atcaagtacc
     3061 gcctggcggc cgcctacctg aagggccagg agatagccaa ggccgtttcc ctcctgaacg
     3121 agatccagca gatctacccg aactacaagg atacgccggc ccagctcgcg aagtacaagg
     3181 agctgaactc caaccggaac ctgcaggtgt acctgctgtc ggccacgagc gacttcatca
     3241 cgctgtgccg caagataacc ctgacgttct tccccaaggc caaggtaaag atcaccgata
     3301 tctcggtgca gaagaacgac tgggccgaca tcctggccga ggtcgagacg agccgctggt
     3361 cggacgtggt gctgttcagg atgatacgtt cgaccggtac gataggcgag ctggtgctac
     3421 gcgacttcca tgcgaggatc aaggacctga aggccggtaa gggctactgc atcaccgccg
     3481 gcgtcttctc cgacgagtcc aagaaattcg tcgaggccag gctgatcgac ctcatggaga
     3541 aggacaagct gatgacgctc ctcaacaaca tcgactcgca gcagaagggg ctcctgataa
     3601 cggactgatc gcgaggctcc gtccgttgac gcgtcgtcgt tgacgcggcg tcgttgacgc
     3661 gccgccggac gaggggtagc atggctccat gttcgaacca tcggtatatt ccgggcgcag
     3721 gcgcgccctg gtcgcggccc tggcgtcgcg aggcgccctg gacggcctcg tcgcctttcc
     3781 gggcaataac gacagccccg tcaattatcc cgacaacgcg taccgcttca ggcaggactc
     3841 gtgtttcctc tactatttcg gcctggccga gcctggcctg gccgcggcga tcgatctggg
     3901 cacgggccgc gcgacgctgt acgccgacgc tctgacgatc gacgacctgg tctggaccgg
     3961 accgaggccg ggcgtcgacg agtaccgggc gctctgcggg gccgacgcgg tcaagccgag
     4021 gtcggcgttc ggtacggacc tggcggccgc gagaggtcga tgcctgttca cgccgccgta
     4081 tcgggcggac gcggccatcg agctcggcga ggcgcttggc atcgctcccg gagccttgtc
     4141 cgcggcggct tcgaacgcct tgataacgtc cgtcgtcgcg cagcgagaga taaaggaaga
     4201 ccgggagctc gccgacatcg agcgggcggt cgatgtgacg atagacatgc atcgggcggt
     4261 gctagcggcc gccaggccgg gcgttaccga ggcggcgatg atggccgagg cctaccgggt
     4321 ggcgtacgcc gggggaggca tgccggcctt tcccgcgata gcgacgacga agggctcggt
     4381 gctgcacaac cacggctacc cgggaacgct tacgagcggc ggcctgttcc tgctggacgc
     4441 gggcgccgag accgcagaag gctacgcggg cgacctgacg agcaccttcc cgataggcgg
     4501 gcgctacgac gataggcagc gcgccgtcta cgacatcgtg ctacgggccg gcgaggcctg
     4561 ctcgtcgatg atggcccctg gcgtgccgta ccgcgactgc cacctggccg cggcccgggc
     4621 catcgccgag ggcctctccg ggctcggggt gatgcgcggc gatgtggacg aggccgtggc
     4681 ggccggcgcc cacgcctgct tcttcccgca tggggtgggg caccagatgg ggctcgacgt
     4741 gcacgatatg gagaacctcg gcgaggtgaa cgtcggttac gacggccagc ctaagagcgc
     4801 ggagttcggc ctcaagagcc tacgcctggc caagccgctc aaggctggcc tggtcgtgac
     4861 cgtggagccc ggcgtctact tcatcgaggg gctgatcgcg gcctggaagg cggagaagag
     4921 gcatgcgtcc ttcattaact acgccgaggc ggagcagtgg ctgcccgtcg gcggcatccg
     4981 caacgaggag gactgggtcg tcgtcgacgg caagggcggc ggcgtcggga gcggcgcgcg
     5041 caggctcggg aagcccttcg ataagtcagc cgcagctatg gagggctacc gggcctgatc
     5101 ggccgctcgg gctagatgaa gctgacccgg gtgtccttga ggtgcctgga ctcgagaatg
     5161 gcggcgatgc gcttgactac gttgcgccgt atctccagct cgagcggcat ggtcggatcc
     5221 tggtgagcct cgttgatgcg cgtgccgacg acgaactgga tgacgtccga gtcgagcatc
     5281 agctcgagga ggcgggtcgc ggcgttgcgc cggcccggcg cctcctcctg gcccgcctcg
     5341 agcagggccg ccacgcgcgc gagggtaagg atgccttcgg tgaccaggtc ggcgcccggc
     5401 atggtggagg atggcggtat ctccgggtcg aggttggata ggtccacccg gatggccagt
     5461 ccggattccc gggcgatgat attcgcggtc gtgcctccgg ctatggcttt gcggccgtcg
     5521 aacgaggcga agatgcgggt catctcccga tcgcggtcct tggagaccgg cgggccggtc
     5581 agcacgagga ggcgcctcgg gtggcgccag tacgccacgc cgcaggttat gtcatccttc
     5641 ggggcccagg cgtccttggc ggtggcttcg tgcacgatag tcctggcgag ctccctcgcg
     5701 gagacgccgt cgggggcttc cgccacggcc gccgtagcga agcgcttgac cgcctcgtcg
     5761 ccccagccca gggggtaact cctcgtaccc atgcccgcct gcgtgacgcc gtcggagtac
     5821 agcacgacgc ggtctcccgg ctcggcgtcg aagctggaca gcttgagcac cgcgtcgccc
     5881 ttggctccgc cgcggccgcg aggtagcctg agctccgtct tttccgggag gagagcctgg
     5941 ccgcgacgca ggagcaggta cggcggatta tcgtactcca cgacgcgcac cgagccgccg
     6001 ggggccacat cgacgatggt gaaggtcgcg tagctgattc tacgctccga gcagaccggt
     6061 agcgagcgca tgacgaactc ggcgacgcgg cggagcggga tgtcggcggc gacgcaacgc
     6121 gcggccatcg tcgccgtcag cgtggcgagc acgccggcct tgataccgga gcccaggccg
     6181 tcggacagca cggagatgac gcggccgtcg tcgcacttga tcgactggaa cgcgtcgccc
     6241 ggcgcgctct gcccgcgctt ggatacctgg tgatgcgata gttcgaggaa ggagtccttc
     6301 atggccggct agccgcgctt ctggcggtcg cggctc
//
LOCUS       DAOPHV010000073         6322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_16036_length_6322_cov_3.920217, whole genome shotgun sequence.
ACCESSION   DAOPHV010000073 DAOPHV010000000
VERSION     DAOPHV010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16036_length_6322_cov_3.920217"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="PLQ29_03150"
     CDS             complement(<1..1055)
                     /locus_tag="PLQ29_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPG85673.1"
                     /translation="MSFVHLHVHSDYSLLDGAASVAGLVERAVSFGMPGLALTDHGNM
                     FGALKFYKECKRVGINPIIGSEFYVAGATRRDKTGTEGGNKYYHLVLLAKDEIGYRNL
                     TMLSSRSYTEGFYYKPRIDWELLESYHEGLICSTACIAGEVPQYVLLGKPEEAERVAA
                     RYRDLFGAENYFLELQDHAMDEEARVNKALVPMARRLGVGLIATNDVHYLDRSDAAAH
                     DALLCVGTNRKVSDQSRMRFPCPDFYLKGPAEMSDLFKEVPEALSNTLLVNEMVGLEI
                     PLPGPLLPEFEIPSVYSGDDDRQAGPEVESVRAFLANMSDDAVCKPNPRYGQAPVEEL
                     APGVRAALAERLAMPVTR"
     gene            1170..1781
                     /locus_tag="PLQ29_03155"
     CDS             1170..1781
                     /locus_tag="PLQ29_03155"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPG85674.1"
                     /translation="MEKGVSQVKARATSFKDFVDAYAGLFDARPAGGDYVRMAWIVSP
                     YGPLLAGADSSSLRFLDFADRAGIDAHIASLRISCRLPLVPGENALIDRLRDELASYF
                     SGALREFSLPVASAGTPFQELVWRELRGIPYGGTLSYGELARRIGSPGASRAVGAANG
                     SNRVLILVPCHRVVNANGTPGGYSGGSERKRSLLELERAALAT"
     gene            1883..3250
                     /gene="lpdA"
                     /locus_tag="PLQ29_03160"
     CDS             1883..3250
                     /gene="lpdA"
                     /locus_tag="PLQ29_03160"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPG85675.1"
                     /translation="MYDIVILGGGPGGYVAADRAAALGLKTALIERDALGGTCLNRGC
                     IPTKSLLAGAKTYRHAIDSAQMGVTCQGVSYDLAAAMAWKAQTVSRLVQNVEFMMKKR
                     GVEVIRGTGRLTGASSLAIAETGLAVEGRHIVVASGSEPARPPIPGVAGNPKVVTSDE
                     LLAIDRLPESIVVIGGGVIGIEFAAYFSALGVKVTVIEMLPEILPFMDAELAAVYKRA
                     LKGVTIATGATVTCVEGGSVRYRKGDAAEETTATGELVLLATGRRPVLAGIGLEAAGV
                     DFSPKGVKVDDSCRTNVPGVWAIGDATGRSLLAHSASAMGRAVAELVASGDAKCDANL
                     PWKALPWVVYGDPEAAGVGMTEAEALAAGYDAVKSSLPARANGRFVAERGVTGHGLCK
                     LVADKASGRLLGAHIVAPYAGEMIWGMQYAIARGATVGELERTVFPHPTVSELFHDAL
                     SALEL"
     assembly_gap    3358..3457
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3470..4183)
                     /locus_tag="PLQ29_03165"
     CDS             complement(3470..4183)
                     /locus_tag="PLQ29_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG85676.1"
                     /translation="MEFVDVAYRYKGERADALTGFSLSVRKGSVTALLGPNGSGKSTA
                     LGLGAGWLEPSSGSVVKDGQAAFLPQSERLAFSFSCIEYVVFGRAPHLPYLAVPTGDD
                     EARAEAALAEVGMADKAGRRITALSGGELQLVRIARALVQEASYVILDEPSDMLDPVH
                     AATIGRAIRRLADSGAGVLLSTHDLAFALACADEAATLRAGRLLAVGPTADIVEPRGL
                     EALYGTPFELRSLPTPVLD"
     gene            complement(4186..5181)
                     /locus_tag="PLQ29_03170"
     CDS             complement(4186..5181)
                     /locus_tag="PLQ29_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPG85677.1"
                     /translation="MSRAGRRVALAAAALALALAFVAALLFGRYPSPGFTDPRSLLTD
                     DMAARLMVLVRLPRVLSALLLGAALGASGAAMQFVFANPLVDAGFLGVSQGASFGAAL
                     AMLLGGGPFWLFGSAFGFSILALVLSVSMASRIRFGGAVLRLILSGMAVSAFFSALVA
                     LIKYGADPLSTLPEITYWLMGGLSGANWRALSVAAPVALGSVVLLVAMRWRIALLSLD
                     EATAASLGARPALERAVVLGAAAAGVAAVTAMAGVVAWVGLIVPHLARLAFGGDGSPT
                     VPGSALLGAFFVVVCDAISRGLFQGELPLGVTTSLVGTIAFFALLMSRRVHVERG"
     gene            complement(5178..6182)
                     /locus_tag="PLQ29_03175"
     CDS             complement(5178..6182)
                     /locus_tag="PLQ29_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPG85678.1"
                     /translation="MKTKHTMLAIAVMALASAAGAQTPSRVVMGGKAVTMVADAVYMF
                     PSARPRVVAIGGTDQGLGVFLETVAPGFSALPSFPRQAGVEVYASHRPDLVILKSSLK
                     KSLGAGLEALGIRTLYLSLETPDDYYAELALLGRTFGDEKRAAELIGYYQNVVATAAG
                     ASAAATKKGDKPRVLLVQASGDGFEVPPDGWIQTTLVEMAGGQAIWKGANPGGGWAKV
                     GPEQIAAWNPDVLLVVSYSESATDAAARMAADPRFSSLKAAKTGRLHGFAQDFLSWDQ
                     PDARWGLGLLWLTDALHPGSMPGYTAESSARRFYALAYGFDAAAFAAQVAPRLRGVLR
                     "
BASE COUNT         1039 a         2137 c         2130 g          916 t
ORIGIN      
        1 cgggtgaccg gcatggccag gcgctcggcg agggcggccc ggacgccagg cgccagctcc
       61 tccaccggcg cctggccgta cctcgggttg ggcttgcaga cggcgtcgtc cgacatgttc
      121 gccaggaagg cccgcacgct ctccacctcc ggcccggcct gcctatcgtc gtcgcccgaa
      181 tagacgctcg gtatctcgaa ttccggcagg agcgggccag gcagcggtat ctcgaggccg
      241 accatctcgt tgacgaggag cgtgttcgac agggcctccg gcacctcctt gaagagatcc
      301 gacatctcgg cggggccttt gaggtagaaa tcggggcacg ggaagcgcat gcggctctgg
      361 tcggagacct tgcggttcgt gccgacgcag aggagggcat cgtgcgcggc cgcgtcggaa
      421 cgatccaggt agtgcacgtc gttcgtcgct atgagcccga cgccgaggcg cctggccatg
      481 gggacgaggg ccttattgac acgggcctcc tcgtccatcg cgtggtcctg caactccagg
      541 aagtagttct cggcgccgaa caggtcgcgg tagcgcgcgg cgacccgttc cgcttcctcg
      601 ggcttgccga gtaggacgta ctgcggcact tcgcccgcta tgcacgcggt cgagcagatc
      661 aggccctcgt ggtagctctc gagaagctcc cagtcgatgc gcggcttata gtagaagccc
      721 tcggtatacg aacgcgacga cagcatggtc aggttgcgat acccgatctc gtccttcgct
      781 agcagtacca ggtggtagta cttgttaccg ccctcggtgc cggtcttgtc tcgcctcgtc
      841 gccccggcga catagaattc gctaccgatg atggggttga tgcccaccct cttgcattcc
      901 ttgtagaact tgagggctcc gaacatgttg ccatggtcgg tcaaggccag gccgggcata
      961 ccgaacgaga ctgcccgctc tacgagtccg gcgacgctcg cggcgccgtc gaggagggaa
     1021 taatcgctat gtacgtggag gtggacgaac gacatggccg cagtatacgc gtcgggcgcc
     1081 gggccgtaaa gacgaggccg ccggtctttt gtatggagct cgatcacttt tctatagcgc
     1141 gccacggcct ttacggctat catgtggcca tggaaaaagg ggtgtcccag gtaaaggctc
     1201 gagctacctc cttcaaggac ttcgtcgacg cctacgccgg cctcttcgac gccaggccag
     1261 ccggcggcga ctatgtgcgc atggcctgga tagtctcgcc ctacgggccc ctgctggccg
     1321 gggccgattc gtcgtccttg cgctttctcg acttcgccga tcgcgccggg atagacgccc
     1381 atatagcctc gctcaggata tcgtgccgcc tgccgctcgt ccccggggag aacgccctga
     1441 tcgaccggct acgcgacgaa ctggcctcgt acttctcggg cgccctccgc gagttctccc
     1501 taccggtggc ctcggcggga actcccttcc aggagctcgt ctggcgcgag ctacgcggca
     1561 taccctatgg cgggaccctg agctacggag agctggccag gcgcatcgga tcgcccggag
     1621 cctccagggc ggtcggggcg gcgaacggct cgaaccgggt gctgatactc gtaccctgcc
     1681 accgggtggt caacgcgaac ggcacgccgg gcggctactc gggcggctcc gagcgcaagc
     1741 gatcgttgct cgaactcgag cgcgccgcgc tcgccacctg atccgtccgc gctcgccacc
     1801 tgatccgtcc tgcatcgacc cggcggttga ccttccgagc cgcctcgtta gacaatgcgc
     1861 ggtcttctaa aaggaggaga ccatgtacga tatcgtcata ttgggcggag gacccggtgg
     1921 ctacgtcgcc gcggaccggg ccgcggcgct cgggctcaag acagctctga tagaacgcga
     1981 cgcgctcggc ggtacctgcc tgaaccgcgg ttgcatcccc acgaagagcc tgctcgccgg
     2041 ggccaaaacc taccgccacg ccatcgactc ggctcagatg ggcgtcacct gccagggcgt
     2101 ctcgtacgac ctggccgccg cgatggcctg gaaggcccag accgtctcgc gcctcgtgca
     2161 gaacgtcgag tttatgatga agaagcgcgg cgtcgaggtg atacggggca ccggccgcct
     2221 gaccggcgcc tcttccctgg cgatagcgga gaccggcctg gccgtcgagg gccgccatat
     2281 cgtcgtcgcc tccggatccg agccggcccg gccgcccatc cccggcgtcg cgggcaaccc
     2341 gaaggtcgtc acgagcgacg agctcctggc catcgaccga ctgccggaga gtatcgtcgt
     2401 catcggcggc ggcgtgatag gcatcgagtt cgcggcgtac ttctcggccc tcggcgtcaa
     2461 ggttacggtc atcgagatgc tacccgagat cctgccgttc atggacgccg agctggccgc
     2521 cgtctacaag cgcgcgctca agggcgtcac gatagccacg ggcgccacgg tcacctgcgt
     2581 cgaaggcggc tcggtcaggt atcgcaaggg cgacgccgcg gaggagacga cggcgaccgg
     2641 cgaactcgtg ctgctcgcga ccgggcgcag gccggttctc gctggcatcg ggctcgaggc
     2701 ggcgggagtg gacttctccc ctaagggcgt caaggtcgac gactcgtgcc gcacgaacgt
     2761 acccggcgtc tgggctattg gcgacgccac gggccgctcc ctcctggccc actcggccag
     2821 cgcgatgggc cgggcggtcg cggagctcgt tgcctcgggc gacgcgaagt gcgacgcgaa
     2881 cctcccctgg aaggccctgc cctgggtcgt ctacggcgac ccggaggcgg caggcgtcgg
     2941 catgaccgag gccgaagcgc tcgccgccgg ctacgacgcg gtcaagtcgt cgctaccggc
     3001 cagggccaac ggtcgcttcg tcgcggagcg aggcgtgacc ggccacggcc tgtgcaagct
     3061 ggtggccgac aaggcctccg gcaggctact cggggcgcac atcgtggcgc cgtacgccgg
     3121 cgagatgatc tggggcatgc agtacgccat agcgcgcggc gccacggtcg gcgagctcga
     3181 gcggaccgtc tttccccacc cgacggtctc cgagctgttc cacgacgcgc tgtcggcgct
     3241 cgagctgtag cgagtagccg tcccgggcag ccgttcctgg tagttggccc gatagccggc
     3301 cgcggcccga gtcttcagtc caggaccggc gtcggcagcg accgtagctc gaacggcnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg cccgagtctt cagtccagga
     3481 ccggcgtcgg cagcgaccgt agctcgaacg gcgtaccgta cagcgcctcg aggccgcgag
     3541 gctcgacaat gtcggccgtc ggtccgacgg ccaggaggcg cccagcgcgg agcgtcgccg
     3601 cctcgtcggc gcaggccagc gcgaaggcca ggtcgtgggt ggacaggagc acgccggcgc
     3661 ccgagtcggc cagccgcctg atcgcccggc ctatcgtcgc ggcgtggacc gggtcgagca
     3721 tgtcgctcgg ctcgtcgagg atcacgtacg acgcttcctg gacgagggcg cgggcgatac
     3781 gcacgagctg cagctcgccg ccggacaagg cggtgatgcg gcgccccgcc ttgtcggcca
     3841 tgccgacctc ggccagggcc gcctcggccc gcgcctcatc gtctccggtc ggcacggcca
     3901 ggtacggcag gtggggcgcc cggccgaaga ccacatactc gatgcaactg aacgagaagg
     3961 ccaggcgctc cgactgcggc aggaaggccg cttggccatc cttgacgacg gaaccggagc
     4021 tcggctcgag ccagccggca ccgagtccga gggccgtcga cttgcccgag ccgttcggcc
     4081 cgagcagggc cgtgacgctc cctttgcgga cgctcaggct gaagccggtc agggcgtccg
     4141 ccctctcgcc cttgtagcgg tatgcgacgt ctacgaattc catcgtcatc ctcgctccac
     4201 gtggacgcgc cggctcatga ggagggcgaa gaaggctatc gtgccgacga gcgaggtcgt
     4261 cacgccgagc ggcagctcgc cctggaacag cccccgcgag atggcgtcgc agaccaccac
     4321 gaagaacgcg ccgagcaggg ccgagccggg cacggtcggg gagccgtcgc cgccgaaagc
     4381 cagcctggcc aggtggggca cgatcagacc gacccaggcg acgacgccgg ccatcgccgt
     4441 gacggccgcc acgccggcgg ctgccgcgcc aagcacgacc gcccgctcca gggcgggcct
     4501 ggcgccgagc gaagcggccg tcgcctcgtc gagggacagg agcgctatcc tccagcgcat
     4561 cgcgacgagc aggacgaccg agccgagggc taccggcgcg gccacggaca acgctcgcca
     4621 attggcacca gacaagccgc ccatcagcca gtaggttatc tccggcaggg tcgacagggg
     4681 atcggccccg tatttgataa gcgcgacgag ggccgagaag aaggccgaga cggccatacc
     4741 cgataggatc aagcgcaaca cggcgccgcc gaagcggatt cgcgaagcca tggataccga
     4801 caagacgagg gcgagtatcg agaagccgaa ggccgaaccg aacagccaaa acgggccgcc
     4861 gccgaggagc atcgccaggg ccgccccgaa ggacgcgccc tgcgacacgc cgaggaaacc
     4921 cgcgtcgacg agggggttgg cgaagacgaa ctgcatcgcc gcgcccgagg ccccgagcgc
     4981 ggccccgagc agtagcgccg acagtacgcg cggcagcctg acgagcacca tcaggcgggc
     5041 ggccatgtcg tccgtcaata gggaccgagg atcggtgaag cccggagacg ggtagcggcc
     5101 gaacaacagg gcggcgacga aggccagggc cagcgccaac gcggcggccg caagggccac
     5161 ccgtcgtcca gctcggctca ccgaagaacg cctcgcagtc gcggagctac ctgggccgcg
     5221 aaggccgcgg cgtcgaaccc gtacgccaga gcgtagaaac gcctagccga cgattccgcc
     5281 gtgtaaccgg gcatcgagcc gggatggagg gcgtcggtca gccagaggag gccgaggccc
     5341 caacgcgcgt ccggctggtc ccaggacagg aaatcctggg cgaagccgtg gagccgaccg
     5401 gtcttggccg ccttgaggct cgagaagcgc ggatcggcgg ccatgcgtgc ggccgcgtcc
     5461 gtggcgctct cgctatacga cacgacgagg aggacatcgg ggttccaggc cgcgatctgc
     5521 tccgggccca ccttggccca gccgccgccg gggttcgccc ccttccagat cgcctgtccg
     5581 ccggccatct cgacgagcgt cgtctggatc catccgtccg gcggcacctc gaagccgtcg
     5641 cccgaggcct gcaccaggag gacgcgcggc ttgtcgccct tcttcgtagc cgccgccgag
     5701 gcgccggcgg ccgtcgcgac gacgttctgg tagtagccga tgagctccgc ggcccgcttc
     5761 tcgtcgccga aggtcctacc gagcagggcc agctcggcgt agtagtcgtc gggcgtctcg
     5821 agggacaggt acagggtcct gatcccgagc gcttcgagcc cggcgccgag acttttcttg
     5881 agactcgact tgagtatgac gaggtccggc ctatgggacg cgtatacctc gacgccggcc
     5941 tggcgcggga acgacggtag cgcggagaaa cccggcgcaa ccgtctccag gaacacgccg
     6001 aggccctggt cggtgccgcc gatcgctacg acacgcgggc gcgcggaggg gaacatataa
     6061 acggcgtcag cgaccatcgt gacggccttg cctcccatga ccacccgcga gggcgtctgg
     6121 gctcccgcag ccgacgccag cgccatgacg gcgatggcga gcatcgtatg cttcgttttc
     6181 atgccgtaat cctagcacgc caaagcgaat cgacacaagc tcattcagtg agtattctgg
     6241 ctatagcctc tcgatacgcc tcgggcgtat ccaggtcctc gaagatgccg tcgtcgtcca
     6301 cctcgacggc agaccacggt ag
//
LOCUS       DAOPHV010000074         6298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_16129_length_6298_cov_3.826526, whole genome shotgun sequence.
ACCESSION   DAOPHV010000074 DAOPHV010000000
VERSION     DAOPHV010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16129_length_6298_cov_3.826526"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PLQ29_03180"
     CDS             complement(<1..770)
                     /locus_tag="PLQ29_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG85679.1"
                     /translation="MLRLFKYLKPYALMVALALLMEFASSMAELSLPALMSVIVDEGV
                     ATGDIALIWRTGARMLAIAFIGVAANLTGGFFAARSAMGFGKTLRSKVFSRVSSFSLR
                     ELDRFGTSSLITRSTNDITQVQQVTFMMQRMMAKAPIMAIGGIAMALATEPRLAWILL
                     VVIPILGAAIVIIALKGLPLFKSIQAKIDALNRVMRENLSGIRVVRAFDRVEHERARF
                     DGVNADLTATSLKVNRIMAFVPAFITVMMNLTSVMIVW"
     gene            complement(774..1274)
                     /locus_tag="PLQ29_03185"
     CDS             complement(774..1274)
                     /locus_tag="PLQ29_03185"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HPG85680.1"
                     /translation="MIDDIRDERPIAERLFEAFRAIGPFKRRPSLGHAQRPGEFQVMH
                     RLSHEPPESGMRVGDLAAWLSVKPPTVTKAVDALVARGLVERYADEADRRVIRVRLSP
                     DGRTLDSSFREQALSEIRGLVDYLGEDDSATLARLLTRAAEYMNERYGRSCSGAHGRA
                     KERGAD"
     gene            1411..3273
                     /locus_tag="PLQ29_03190"
     CDS             1411..3273
                     /locus_tag="PLQ29_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HPG85681.1"
                     /translation="MSFLPTTRAELAARRLDGLDFVIVTADAYVDHPSFGAALIGRRL
                     ERLGYSVGVIARPDPTDVEAFRALGRPSLAFLVAGGAIDSMVCKYTANRKPRSTDEYA
                     PGGDPSLCRGADGSIRAFAVNGRPASARPDRAVIAFAGKCREAYKGVPVIAGGLEASL
                     RRLSHYDYLSDTVRRPIIVDSKADLIVYGMGERAIAEAARVLAEARSRSDAFDPAALR
                     GIRGTVWRCSRREDVPEGAVSLPDHEAAAADGRAFAESFAAQYRNTDPVSARPLVEAA
                     AGQFVVQEPPQLPLSRAELDELYGLGFERAWHPAYDRYGGVPALAEVRFSIASSRGCY
                     GACAFCALAFHQGRVVTSRSKESIVDEARLLTGLTGFKGYIHDVGGPTANFRGPACDK
                     QAGGGACVDRRCLAPEPCPALKADHRDYVSVLRAVRAVPGVKKAFVRSGVRFDYVMAD
                     SDRTFLRELAAHHVSGQLKVAPEHVSDRVLRLMGKPPRRVYDAFAGEWSRVNAELGLK
                     QFLVPYYIASHPGAELSDAIELAEYLRDSGFVPDQVQDFYPTPGTLATAMYRSGLDPL
                     TMVPVYVARGAKERSYQRALLQYSKPENRKLVIEALRLAGRTDLIGRGPKCLVS"
     gene            3380..6238
                     /locus_tag="PLQ29_03195"
     CDS             3380..6238
                     /locus_tag="PLQ29_03195"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPG85682.1"
                     /translation="MTKRMRVALAPVLAIILALSACVGAPEPAPGVLDNVSTLPGGSA
                     YRPSDPLPLAPELTKRVLPNGLTYYVRRNGNPGGRAVMFLIVNSGSTNELDDQSGYAH
                     FVEHMAFNGTESFPENELVGYLRSIGMDFGAEINAHTTREETLYTLQMPLSDPEFFDT
                     GLRVLKEWATAVTFDPAEVEKEKGVILEELRLTLGPDETARVREVRGLLAGSAHEDRE
                     PIGYEASIKAATAERLRAFYEEHYRPDRMAVIVVGDIDVREVSQKIEREFSFPARDGR
                     VQPRPTFAVEPTRDLGFVATFDPSIERSVVTYRKIVPYEPETVIGDYERLLKIRIAAE
                     AIRMRLSDLVRSGGSPWRDAYFDDDYFYGRTRLYSFSLSSAAGEELGAFGGLAREVER
                     IRRFGFTDSEFNRTADLYRRWLGTLDVEDDDLKSFSFAEEYVRNFMYGEPVPGVVNER
                     VYIKQVLDELTLGELNAAAAEILAADEGFVGVRAKTGTGNAALDEAAFERLLREARTA
                     ALEPLASGGDEVGLFDDLPEPGSIVSDRRLADGITELMLSNGARVLLRPTSYDKDSIS
                     FLAWSAGGYSTLPVGLQTAAAFAPSLLTAAGLGGMSATRVDEVTASLNAGLQWSIGEN
                     GEILAGKTATVDLEAFLRLVYLTSAEPGRDAMAFNAARDRLASQIGPYVRDPSYRFES
                     SWSQDLFGGNARAAPLDEAATRAVDFDETRALAVSALADASEFTYVLVGDFKLDEAKA
                     LVAKHIGAIPAGSPDTPEWIAPLSPRDGGRRVDYRLSREPRASVRMVWAGEAPWSWED
                     EQTLELLAQALNNRLLDALREDLGGTYVVSTRASFAREPIEQFSLIVQFDTDPARVEE
                     LIAEVKAEVASLAAGTFDPIYASQVQAAARRDFGGRSRTNDFWANGLGNAIASGLDLD
                     ILARAERTAGLADPAAFQALAAGLFADDASFVYVMLPE"
BASE COUNT          999 a         2180 c         2086 g         1033 t
ORIGIN      
        1 aaccagacta tcatcaccga ggtcaggttc atcatcaccg tgatgaaggc cggcacgaag
       61 gccatgatgc ggttgacctt gaggctggtg gcagtcaagt cggcgttgac gccgtcgaag
      121 cgggcccgct cgtgctcgac ccggtcgaag gcgcgcacga cccggatgcc ggagaggttc
      181 tcgcgcatca cgcggttgag cgcgtcgatc ttggcctgga tggatttgaa taggggtaga
      241 cccttgagcg cgatgatgac gatggccgcg ccgagtatcg ggatgacgac gagcaggatc
      301 cacgccaggc gcggttcggt agccagcgcc atcgcgatgc cgccgatggc catgatcggc
      361 gccttggcca tcatccgctg catcatgaag gtaacctgct ggacctgggt gatgtcgttc
      421 gtcgacctgg tgatcagcga cgaggtgccg aagcggtcga gttcccggag cgagaacgac
      481 gatacgcgcg agaagacctt ggagcgcagc gtcttcccga agcccatcgc cgacctggcc
      541 gcgaagaagc cgccggtcag gttggccgct acgcctatga acgctatggc cagcatccgg
      601 gcgccggtgc gccagatcag cgcgatatcg ccggtcgcta cgccctcgtc cacgatgacc
      661 gacatcagcg ccggcaagct cagctccgcc atcgagctgg cgaattccat cagaagcgcc
      721 agcgctacca tgagcgcgta cggcttcagg tacttgaaga gtctcagcat gtcttaatcc
      781 gctcctcgtt ccttggctcg gccgtgggct ccgctacagg agcggccgta ccgttcgttc
      841 atgtactcgg cggcccgagt caggagccgc gcgagcgtcg cgctgtcgtc ctcgcccagg
      901 tagtcgacga ggccccgtat ctcgctgagc gcctgctcgc ggaatgagct gtccagcgtt
      961 ctaccatcgg gagacaggcg caccctgatg acccgcctat ccgcctcgtc ggcgtagcgc
     1021 tcgaccaggc ccctggcgac cagcgcgtcg accgccttgg tcacggtcgg cggcttgacc
     1081 gacagccagg ccgccaggtc gccgacgcgc atgcccgact cggggggctc gtgcgacagt
     1141 cggtgcatga cctggaattc gcccggccgt tgggcatgcc cgaggctcgg ccggcgtttg
     1201 aacggtccga tggccctgaa cgcctcgaag agccgctcgg cgatcggccg ctcgtcccgt
     1261 atatcgtcga tcatgttcgt cctcgtagct tcgcatcctc atgcaattag ccttgctaaa
     1321 agttagcctt gctaaatata ttaacgccgg cttgcctggt caaggctatg accacgcgac
     1381 caagaaggcc cgggagggta tactacgggc atgtcgttcc tccccaccac gcgagccgaa
     1441 ctggccgcca ggcgcctcga cgggctcgat ttcgtcatcg tcacggcgga tgcctacgtc
     1501 gaccatccgt cgttcggcgc ggccctgatc gggcgcaggc tcgagcgcct cggctactcg
     1561 gtcggcgtca tagccaggcc cgacccgact gacgtcgagg ctttccgcgc cctcggtcgt
     1621 ccgtccctgg ccttcctcgt cgctggcggc gcgattgact cgatggtctg caagtacacc
     1681 gccaaccgca agccgcgctc gaccgacgag tacgcccctg gcggcgaccc gagcctctgc
     1741 cgcggcgccg acggctcgat acgggctttc gcggtcaacg gccggcctgc gagcgccagg
     1801 ccggaccggg cggtgatcgc cttcgccggc aagtgccgcg aggcctacaa gggcgtgccg
     1861 gtgatagccg gcggcctcga ggcctcgctg cgccgcctgt cgcactacga ctacctgtcg
     1921 gatacggtgc gcaggcccat catcgtcgac tccaaggccg acctcatcgt ctacggtatg
     1981 ggagagcgcg cgatcgccga ggcggcccgc gtcctcgccg aggcccgctc ccgatccgac
     2041 gcgttcgatc cggcggcgct acgaggcatc cgcggtacgg tatggcgctg ctcgcgccgg
     2101 gaagacgtgc cggagggcgc cgtgagccta cccgaccacg aggccgccgc cgcggacggc
     2161 cgggccttcg ccgagtcgtt cgcggcgcag taccgtaaca ccgacccggt atcggcccgg
     2221 cccctcgtcg aggcggccgc cggccagttc gtcgtgcagg agccgcctca gcttccgctg
     2281 tcccgcgcgg agctggacga gctatacggc ctcggcttcg agcgcgcttg gcacccggcc
     2341 tacgaccggt acgggggcgt accggccttg gccgaggtgc gcttcagcat agcctcgagc
     2401 cgaggctgct acggcgcttg cgccttctgc gctctcgcgt tccatcaggg ccgcgtcgta
     2461 acgagccggt caaaggagtc catcgtcgac gaggcgcggc tcttgacggg cttgaccggc
     2521 ttcaagggtt atatccatga tgtcggcggg ccgacagcca acttccgagg gcccgcctgc
     2581 gataagcagg ccgggggcgg cgcctgcgtc gatcggcgct gcctggctcc cgagccatgt
     2641 ccggcgctca aggccgacca ccgggactat gtctccgtgc tgcgggcggt ccgcgccgta
     2701 cccggcgtca agaaggcctt cgtgcgctcg ggcgtgcgct tcgactacgt gatggccgac
     2761 agcgaccgca ccttcctgcg cgaactggcc gctcaccacg tctccggcca gctcaaggtg
     2821 gcgccggagc acgtgtccga ccgggtgctc cgcctgatgg gaaagcctcc gcgccgcgtc
     2881 tacgacgctt tcgccggcga gtggtcgcgg gtgaacgccg agctcggtct aaagcagttc
     2941 ctggtgccgt actatatcgc ctcgcaccct ggcgccgagc tgtccgatgc gatagagctg
     3001 gccgagtacc tgcgcgactc gggcttcgtg cccgatcagg tgcaggactt ctacccgacg
     3061 ccgggtacgc tcgcgacggc catgtaccgg agcggcctcg acccgctgac gatggtgccg
     3121 gtctacgtgg cgcgcggcgc caaggagcga tcctaccagc gcgccctgct acagtactcg
     3181 aagcccgaga accgcaagct ggtcatcgag gcgctcaggc tggccggccg gaccgacctg
     3241 atagggcgcg ggccgaaatg cctcgtgtcg taacattggt cgtcggcgtg cctttacgct
     3301 atattcaaac gacggcctcg ccccgatggc tcggcactaa ggctcggcca ttaggctggc
     3361 gcgtcgataa ggagtaccca tgacgaagcg gatgcgggtc gccctggccc ccgttctagc
     3421 gatcatactg gccctgtcgg cctgcgtcgg cgccccggag ccagctcccg gcgtcctgga
     3481 caatgtatcg accctgcccg gcggcagcgc ctaccggccg tccgacccgt tgccgctggc
     3541 gcccgagctg accaagcgcg tgctgccgaa cggcctgacc tactatgtcc gacgtaacgg
     3601 caaccccggt ggccgagcgg tcatgttcct gatcgtcaac tcgggctcga ccaacgagct
     3661 cgacgaccag tccggttacg cccacttcgt cgagcacatg gccttcaacg gcaccgagag
     3721 cttcccggag aacgagctgg tcggctacct gcgttccata ggcatggatt tcggcgccga
     3781 gatcaacgcg cacacgaccc gcgaggagac gctatacacg ctacagatgc cgctgtcgga
     3841 tcccgagttc ttcgacaccg gtctcagggt gctcaaggaa tgggccacgg ccgtgacctt
     3901 cgaccccgcc gaggtggaga aggagaaggg cgttatactc gaggaactca ggctgacgct
     3961 cgggcccgac gagaccgccc gcgtgcggga ggtccgcggg ctcctcgccg gcagcgccca
     4021 cgaggaccgc gagcctatag gctacgaggc gtcgatcaag gctgcgaccg ccgagcggct
     4081 gcgcgccttc tacgaggagc attaccggcc ggaccggatg gccgtcatcg tggtcggcga
     4141 tatcgacgtc cgcgaggtct cgcagaagat agagcgcgag ttctccttcc ctgcccgcga
     4201 cggacgggtc cagcctagac ctacgttcgc cgtggagccg acccgcgacc tgggcttcgt
     4261 cgccaccttc gacccgtcca tcgagcgctc ggtcgtcacc taccgcaaga tcgtaccgta
     4321 cgagcccgag acggtcatcg gcgactatga gcgcctgctc aagatacgga tagccgccga
     4381 ggccatacga atgcgcctgt cggacctggt gcgatccggt gggtcgccgt ggcgcgacgc
     4441 ctacttcgac gacgattact tctacggccg gacccggctg tactccttct cgctgagctc
     4501 ggccgccggc gaggagctcg gcgccttcgg cggcctggcc cgggaagtcg agcgtatacg
     4561 ccgcttcggc ttcaccgaca gcgagttcaa ccggacggcc gatctctacc ggcgctggct
     4621 cggcaccctc gacgtcgagg acgacgatct caagagcttc agcttcgccg aggagtacgt
     4681 gcgcaacttc atgtacggcg agccggtgcc cggcgtcgtc aacgagcgcg tgtatatcaa
     4741 gcaggtcctc gacgagttga ccctaggcga gttgaacgcg gctgccgccg agatcctggc
     4801 ggcggatgag ggcttcgtgg gagtgcgagc caagaccggc acgggtaatg ccgcgctcga
     4861 cgaggccgcc ttcgagcggc tgctccgcga ggctaggacc gcggccctcg agccgctcgc
     4921 ctccggcggc gacgaggttg gcctgttcga cgacttgccc gagcccggtt cgatcgtatc
     4981 ggaccggcgc ttggcggacg ggatcaccga gctcatgctg tccaacgggg cgcgcgtgct
     5041 actcaggccg acctcctacg ataaggactc gatcagcttc ctggcctggt cggccggcgg
     5101 ctactcgacg ctacccgtgg ggctccagac ggccgcggcc ttcgcgccat cgctcctgac
     5161 ggcggccggc ctcggcggca tgagcgccac cagggtcgac gaggtgaccg cgtcgttgaa
     5221 cgccggcctc cagtggtcca tcggcgagaa cggcgagatc ctggctggca agaccgccac
     5281 cgtggacctc gaggctttcc tgcgcctggt ctacctgacc tcggcggagc ccggccgaga
     5341 cgccatggcc ttcaacgccg cccgcgaccg gctcgcgtcc cagatcggcc cctacgtgcg
     5401 cgacccgtcg taccgcttcg agtcgtcatg gagccaggac ctgttcggcg ggaacgccag
     5461 ggcggccccg ctcgacgagg cggctacccg agccgtcgac ttcgacgaga cgagggccct
     5521 ggcggtctcg gcgctggccg acgcgtccga gttcacctac gtgctcgtcg gcgatttcaa
     5581 gctcgatgaa gccaaggccc tcgtcgccaa gcacatcggc gccataccgg ccggctcgcc
     5641 cgatacgccc gagtggatag cgccgctatc gccgcgggac ggcggccggc gcgtcgacta
     5701 caggctgtcg cgcgagccga gggccagcgt acgcatggtc tgggccggag aggctccctg
     5761 gtcctgggag gacgagcaga ctctcgagct actggcccag gccctgaaca accggctcct
     5821 ggacgccctg cgcgaggacc tcggcggtac ctacgtggtg tcgacgcggg cctcgttcgc
     5881 cagggagccg atcgagcagt tctcgctgat cgtccagttc gacaccgacc cggccagggt
     5941 cgaggagctg atagccgagg tcaaggccga ggtagccagc ctggccgccg gaaccttcga
     6001 cccgatatac gccagccagg tgcaggccgc ggcgcgtcgc gacttcggcg gcaggagccg
     6061 tacgaacgac ttctgggcca acggccttgg caacgcgatt gcgagcgggc ttgacctgga
     6121 tatactggct cgggccgagc ggacggccgg cctggccgac ccggccgcgt tccaggccct
     6181 ggccgccggg ctcttcgcgg acgacgcttc cttcgtctac gtgatgctgc ccgaataaag
     6241 ccgcgcctct gcgcgaaaaa aaaggcctcc gcgcgagcgg aggccttttt tagcgcgt
//
LOCUS       DAOPHV010000075         6297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_16138_length_6297_cov_4.487985, whole genome shotgun sequence.
ACCESSION   DAOPHV010000075 DAOPHV010000000
VERSION     DAOPHV010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16138_length_6297_cov_4.487985"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLQ29_03200"
     CDS             <1..374
                     /locus_tag="PLQ29_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85683.1"
                     /translation="LPLHAGLSVAGGLVYGGLSTWLTSGDEPFPSSRVWLYHLGSVLG
                     SFLGLGVPYVFGAESPGVIYGGMLVGSLAGSATALWLTRDYTEGRNVGNVAFIPELPR
                     PVALGTDGGFGLYLPLLSARY"
     gene            716..1876
                     /locus_tag="PLQ29_03205"
     CDS             716..1876
                     /locus_tag="PLQ29_03205"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HPG85684.1"
                     /translation="MIQNALPYVAFLIFFAGLLVFLEKTLKLKVFKYVPAIVMLYFFA
                     MLLSTFGVWQKTPQITAAYKALKDGLLPAMIFLMLLRCDLRKIFKLGPRMLLGFFAAT
                     ITIMIGFIVTFAIFKGAFEPGSWKAFAALSGSWIGGTGNMVAIQGALDVPDANMGYTL
                     LIDSINYSIWVMFLLWVVSFAPAFNKWTKANSKVLDEVGQKLGAATEGNRTNVEFVDL
                     IVLLGTSLLVSALSIWLGGMLPKSVFFQSSTWTVLIATAAGVICAMTPLAKLPGSNHL
                     SNLMLYTIVGLIASRANFAELTQAPIYILAGFCILGIHAILLALVAKLCKLDLFTCGV
                     ASLANIGGVASAPILAASYGEALVPIGVLMALMGYIVGTGGGLLVGKVLSIL"
     gene            1943..2362
                     /locus_tag="PLQ29_03210"
     CDS             1943..2362
                     /locus_tag="PLQ29_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3870 domain-containing protein"
                     /protein_id="HPG85685.1"
                     /translation="MYDPSTIYVVGNAKTNTENAITTLYNSFYIGFVLDPATGRIVDA
                     SCSATIRTTDEFVRGIFVGKAMGRDDASLDDEVRRRYHGSSQKAILVAYKDAAKKLAE
                     IQARLGTEGPRNEDQGTAARPHIGTAQEALQDGAQDR"
     gene            2283..3371
                     /locus_tag="PLQ29_03215"
     CDS             2283..3371
                     /locus_tag="PLQ29_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HPG85686.1"
                     /translation="MKIKELRLGRISVPLKKPFKTALRTVESVEDVIVELHADSGEVG
                     YGEAPPTGVITGDTTGAIIGAIQDHLSKAVVGLDVENLDEIMARIDASVVHNTSAKAA
                     VDIAVYDLFGKLCGQPLYKLWGGYRKEFATDITISANGAEQMAADAAEAMALGYRTLK
                     IKLGKDPAQDVARMKAIRAAVGYGVDLRVDANQGWKPKEAVRLIRAIEDAGLALELVE
                     QPVAAHDLEGLKHVTDNVSVPVMADESAFSARDAQRIMELRAADIVNIKLMKTGGVHN
                     ALRVCSLAEVYGVELMIGCMLEAKVSVTAAVHLAAAKKVITKVDLDGPVLCSEDPVDG
                     GAVFAESLVKPNEAPGLGITAIRGLRYL"
     gene            complement(3460..4944)
                     /gene="pepD"
                     /locus_tag="PLQ29_03220"
     CDS             complement(3460..4944)
                     /gene="pepD"
                     /locus_tag="PLQ29_03220"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HPG85687.1"
                     /translation="MHIDELQPRSVWKRFFDICAIPHPSHREAALADALMRWAAERGI
                     EAKRDEAGNVAMSRPAVFGMEDHPGVILQAHLDMVPQKAAESGHDFERDPIRPRLDPK
                     NPAWLVADGTTLGADDGIGVAMALALLEDHELRSGRLECLFTVNEEDGMSGARAIEPG
                     FVTGSLLLNLDGEDEAELTIGCAGAVRTIAELSIPATAAPDGLAWFEAAVDGLLGGHS
                     GVDIDKGRANATLALVGILARTRAELRIASISGGTAANAIPREARAIVGVEAARAAEF
                     RAAFQSEATAARIALGDKDPGLAAALASIAVKPAASLAPKDAASMLTMLGALPNGLVA
                     MEPDMPGLVRTSLNLGKAEGAVAPGTDRFRLETLVMIRSSSDDEKEALAATVERRLAG
                     VAEHGWAVEQRRPAVSPAWSPDPGSPLLGKAVAVYRELFGKDPRVTSTHGGLETGLFR
                     PRFPSWDMLSLGPTIRYPHSPDERVEIASVERSYRFARELVSRL"
     gene            complement(5039..6235)
                     /gene="trpB"
                     /locus_tag="PLQ29_03225"
     CDS             complement(5039..6235)
                     /gene="trpB"
                     /locus_tag="PLQ29_03225"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPG85688.1"
                     /translation="MEDYFKSFPDASGNYGDFGGSFIPPDLQREMDKITDAYYSISKS
                     HEFISELRGIRKHFQGRPTPVYFAKTLSDECGGRIYLKREDLNHTGAHKLNHCMGEAL
                     LATYLGKKKLIAETGAGQHGVALATAAAYFGLECEIHMGEVDIAKEAPNVTRMRILGA
                     TVVPVSAGLKTLKEAVDSAFSAYLEDPVSSIYCIGSVVGPHPFPMMVRDFQRVVGIEA
                     REQFFEMTGEHPDDVVACVGGGSNAMGIFSAYLDDEGVRLYGVEPMGRGPETGDNAAT
                     ITYGKPGVIHGFKCYLLQDEKGEPSPVYSIASGLDYPGVGPEHSMLHDLGKVRYVSVG
                     DDECVDAFYELSRKEGIIPALESAHAVAYAKRLAKADPRRSILVNLSGRGDKDIDFVI
                     AKYGYR"
BASE COUNT         1025 a         2116 c         2025 g         1131 t
ORIGIN      
        1 tcttgccgct ccacgccggg ctcagcgtcg ccggaggcct cgtctacggc ggcctgtcca
       61 cctggctgac ctccggcgac gagccattcc cgagttcgag ggtctggcta taccacctcg
      121 gctcggtgct cggttccttc ctcggactcg gcgtgcccta cgtgttcggc gccgagtcgc
      181 cgggcgtgat ctacggcggc atgctggtcg gctcgctggc gggctcggcg acggcgctct
      241 ggttgacgcg cgactatacc gaggggcgga acgtgggcaa cgtcgccttc atcccggagc
      301 taccccggcc tgtcgcgctc ggaaccgacg gcgggttcgg cctctacctg ccgctcctga
      361 gcgccaggta ctagtcgggc accgtcgtcg gggcgcgaaa cgaaaacggt tgcgcgccgc
      421 gagaatcgac cgtatactcg ccttgccgcg ccctctctcg cgcggtttac tgcttggttt
      481 gcttgggtgt ccttccggca tcgtccgtaa acgaagctag gtaaacggct taaggccgta
      541 ttattctagc cgtccgccta cggagtccgc gatgcccctg tctccgctcg aggcttcgcc
      601 cctctccctc cggccggcgg ctcagcgctc cgcgcgcgaa ggaggccgtc cccccgatga
      661 accatcgtcg tctcgacgat caacgccgca caccaacgaa caggagagaa accctatgat
      721 ccagaacgcg ctaccctatg tcgccttcct catcttcttc gcaggcctcc tcgtcttcct
      781 cgagaagacc ctgaagctca aggtcttcaa gtacgtaccg gccatcgtca tgctctattt
      841 cttcgcgatg ctgctgtcga cgttcggcgt ctggcagaag acgccgcaga tcaccgccgc
      901 atataaggcc ctcaaggatg ggctgctgcc ggcgatgatc ttcctcatgc tgctacgctg
      961 cgatctacgc aagatcttca agctcggccc gaggatgctc ctcggcttct tcgccgcgac
     1021 gatcaccatc atgataggtt tcatcgtcac cttcgccatc ttcaagggcg ccttcgagcc
     1081 cggtagctgg aaggccttcg cggcgctgtc cggtagctgg atcggcggca ccggcaacat
     1141 ggtcgccatc cagggcgctc tcgacgtacc cgacgcgaac atgggctaca cgctcctgat
     1201 cgactcgatc aactattcga tctgggtgat gttcctgctc tgggtcgttt cgtttgcccc
     1261 ggcgttcaac aagtggacca aggcaaattc caaggtcctc gacgaggtcg gccagaagct
     1321 cggcgccgcc accgagggca acaggaccaa cgtcgaattc gtcgacctga tcgtgctgct
     1381 cggcacgagt ctcctcgtct ccgccctgtc catttggctg gggggcatgc ttcccaagtc
     1441 tgtattcttc cagagttcga cctggaccgt gctgatcgcg accgccgccg gcgtcatctg
     1501 cgcgatgacc ccgctggcca agctgcccgg ctccaaccac ctgtccaacc tgatgctgta
     1561 caccatcgtc ggcctgatcg cctcccgtgc gaacttcgcc gagctgaccc aggctccgat
     1621 ctacatcctg gccggtttct gcatcctggg catccacgcc atcctgctcg ccctggtagc
     1681 caagctgtgc aagctcgacc tgttcacctg cggcgtcgcg agcctcgcca atatcggcgg
     1741 cgtcgcgtcg gcgcctatcc tggcggcgtc ctacggcgag gccctggttc cgatcggcgt
     1801 gctcatggcg ctcatgggct atatcgtcgg caccggcggc ggcctcctcg tcggcaaggt
     1861 gctttcgata ctataatcag cgctccgggg ccgtcgcgga gacgcggccc cggaggcagc
     1921 tcgtttcgat ccggaggtag ctatgtacga cccctcgacg atctacgtcg tcggtaacgc
     1981 caagaccaat accgagaacg cgataacgac gctctataat tcgttctata tcggcttcgt
     2041 gctcgacccc gccacaggca ggatcgtgga cgcgtcctgc tcggccacga tcaggacgac
     2101 cgacgaattc gtgcgcggca tcttcgtagg caaggcgatg gggcgggacg acgcatcgct
     2161 cgacgacgag gtccggcgcc gctaccacgg atcgtcgcag aaggccatcc tggtcgccta
     2221 taaggacgcc gcgaagaagc tggcggagat tcaggccagg ctgggaacgg aaggaccccg
     2281 taatgaagat caaggaactg cggctcggcc gcatatcggt accgctcaag aagcccttca
     2341 agacggcgct caggaccgtt gagagcgtcg aggatgtcat cgtcgagctg cacgccgact
     2401 cgggcgaggt aggctacggc gaggcgcctc ctaccggcgt catcaccggc gacacgacgg
     2461 gcgccatcat cggcgccatc caggaccacc tgtcgaaggc cgtcgtcggc ctggacgtgg
     2521 agaacctcga cgagatcatg gcgcgcatcg acgcttcggt ggtccacaac accagcgcga
     2581 aggccgccgt ggacatagcc gtctacgacc tgttcggcaa gctgtgcggc cagcccctat
     2641 acaagctctg gggcgggtac cgcaaggaat tcgccaccga cataacgatc agcgccaacg
     2701 gcgccgagca gatggccgcc gacgccgccg aggccatggc tctcggctac cggacgctga
     2761 agatcaagct cggcaaggac ccggcccagg acgtggcgcg catgaaggcc atccgggccg
     2821 ccgtcggcta cggggtcgac ctacgggtgg acgccaacca gggctggaag cccaaggagg
     2881 ccgtcaggct cattcgcgcc atagaggacg cgggcctcgc gctcgagctc gtggagcagc
     2941 cggtggcggc ccacgacctc gaaggcctca agcacgtgac cgacaacgtc tcggtgcccg
     3001 tgatggccga cgagagcgcc ttctcggcca gggacgctca gcgcatcatg gagctgcggg
     3061 cggccgatat cgtcaacatc aagctgatga agaccggggg cgtgcacaac gcgctgcgcg
     3121 tctgctcgct ggccgaggtc tacggcgtcg agctgatgat aggctgtatg ctggaggcca
     3181 aggtcagcgt gacggcggcc gtacacctgg cggccgccaa gaaggtgatc accaaggtgg
     3241 accttgacgg acccgtactc tgtagcgagg accctgtcga cggaggcgcg gtcttcgccg
     3301 agtcgctcgt caagccgaac gaggcgcccg gcctcggcat aaccgccata cggggattac
     3361 gctacctgta acggctgctt gccgccgtcg cgggctagcg gattctagga ggggcgctcc
     3421 ggctcaggcc gaggcgcccc cattctactc atggcgtctt catagcctcg agaccagctc
     3481 ccgcgcgaag cggtaggagc gctcgacgga ggcaatctcg acgcgctcgt cgggcgagtg
     3541 cgggtagcgt atggtcgggc ccagggacag catgtcccag ctcgggaagc gcggcctgaa
     3601 gaggcccgtc tcgaggccgc cgtgcgtcga ggtgacgcgc ggatccttgc cgaacagctc
     3661 ccggtagacg gcgacggcct tgccgaggag cggactgccc ggatcggggc tccaggctgg
     3721 cgataccgcc ggccgcctct gttcgaccgc ccagccgtgc tcggcgacgc ccgctagccg
     3781 cctctcgacg gtcgcggcca gagcctcctt ctcgtcgtcg gacgagctgc ggatcatcac
     3841 gagcgtctcg agcctgaagc gatcggtacc cggagcgacg gctccctcgg ccttgccgag
     3901 gttgagcgag gtcctgacga ggcccggcat gtccggctcc atcgcgacga ggccgttcgg
     3961 gagcgcgccg agcatggtca gcatcgaggc cgcgtccttg ggagcgaggc tggccgccgg
     4021 cttgacggct atggacgcca gcgcggccgc gagcccggga tccttgtcgc cgagggctat
     4081 gcgggccgcg gtcgcctcgg actggaaggc ggcgcggaat tcggcggccc tcgccgcctc
     4141 gacgccgacg atggccctgg cttcccgagg gatggcgttg gccgccgtgc cgcccgatat
     4201 cgaggcgata cgcagttccg cgcgcgtcct ggccaggatg ccgaccaggg ccaacgtcgc
     4261 gttggcccgc cccttgtcga tgtcgacgcc ggaatggccc ccgagcaggc cgtccacggc
     4321 ggcctcgaac caggccaggc cgtccggggc ggctgtcgcc gggatggaca gctcggctat
     4381 cgtcctgacg gcgccggcgc agcctatcgt cagctcggcc tcgtcctcgc cgtccaggtt
     4441 caggagcagc gaacccgtga cgaagcccgg ctcgatggcg cgggctccgc tcataccgtc
     4501 ctcctcgttg acggtgaaca ggcactcgag ccgaccgctc cggagttcgt gatcctcgag
     4561 cagggccagg gccatcgcca cgccgatgcc atcgtcggcg ccgagcgtcg tgccgtcggc
     4621 gacgagccag gcgggattct tggggtcgag ccttgggcgt atcgggtcgc gctcgaagtc
     4681 gtggcccgac tcggccgcct tctgcggcac catgtccagg tgggcctgca ggatcacgcc
     4741 cggatggtcc tccatgccga agacggcggg gcggctcatc gccacgttcc ccgcctcgtc
     4801 gcgcttggcc tctatgccgc gctcggccgc ccagcgcatc aacgcgtccg ccagggcggc
     4861 ctcgcggtgg gacgggtgcg ggatggcgca tatatcgaaa aagcgtttcc agactgatct
     4921 cggttgtagc tcgtcgatgt gcattcgttg tactcctcca ccgtaatagt accacggatg
     4981 ctcggaggag gcgagcggcg gctcgtacgg cgctcgcctc ccatggtcgc cgtcctcttc
     5041 agcggtatcc gtacttcgct atgacgaaat ctatgtcttt gtctccacgc ccggacaggt
     5101 tgacgagtat cgaacgtcgc gggtccgcct tggcgaggcg cttggcgtag gctaccgcgt
     5161 gggcgctctc gagcgccggg atgatgccct ccttgcggct gagctcgtag aacgcgtcga
     5221 cgcactcgtc gtccccgacc gagacgtagc gcaccttgcc gaggtcgtgc agcatggagt
     5281 gctcggggcc gaccccgggg tagtccaggc cgctcgcgat cgaatagacg ggcgacggct
     5341 cgcccttctc gtcctgcagg aggtaacact tgaagccgtg gatcacgccc ggcttgccgt
     5401 aggtgatggt cgcggcgtta tcgcccgtct cggggccgcg gcccatcggc tcgacgccgt
     5461 agagccggac gccctcgtcg tcgagatagg ccgagaagat gcccatcgcg ttggagccgc
     5521 ccccgacgca ggccaccacg tcgtccggat gctccccggt catctcgaag aactgttccc
     5581 gcgcctcgat gccgacgacg cgctggaagt cgcgcaccat catcgggaag gggtgtggtc
     5641 cgacgaccga gccgatgcaa tagatgctgg agaccgggtc ctcgagatag gcggagaacg
     5701 ccgagtccac ggcctccttg agggttttca gtcccgcaga gaccggtacc accgtcgcgc
     5761 ccaggatcct catcctggta acgttcggcg cctccttggc tatgtccacc tcgcccatgt
     5821 ggatctcgca ctccagcccg aaataggcgg ccgccgtggc gagcgccacg ccgtgctgtc
     5881 cggcgccggt ttcggctatc agtttcttct tgccgaggta cgtggcgagc agggcctcgc
     5941 ccatgcagtg gttgagctta tgcgcgccgg tatggttcag gtcctcgcgt ttcaggtaga
     6001 tacgccctcc gcactcgtcc gacagcgtct tggcgaagta gaccggcgtg ggccggccct
     6061 ggaagtgctt gcggatgccg cgaagctcgg aaataaattc gtgtgacttg ctgatcgagt
     6121 agtacgcgtc ggtgatcttg tccatctccc tctgtaggtc gggcggaatg aagctgccgc
     6181 cgaaatcgcc gtagttgccg ctcgcgtccg gaaaggactt gaaatagtct tccatatgta
     6241 tctcctagta gaaacaatag aggcatcata cccacgattg gcgtatctgt caatagt
//
LOCUS       DAOPHV010000076         6273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_16242_length_6273_cov_4.218237, whole genome shotgun sequence.
ACCESSION   DAOPHV010000076 DAOPHV010000000
VERSION     DAOPHV010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16242_length_6273_cov_4.218237"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1331)
                     /gene="gcvPA"
                     /locus_tag="PLQ29_03230"
     CDS             complement(3..1331)
                     /gene="gcvPA"
                     /locus_tag="PLQ29_03230"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HPG85689.1"
                     /translation="MPFIPNTDADREAMLAAVGARSVEELFSDIPADKRFPKLGLPEG
                     LSELEALREIEAMASKNEVASKSAWFLGAGAYNHFIPAAVPALASRGEFLSAYTPYQP
                     EVSQGTLQAIFEYQSMAAELFGVDTVNASHYDGATALAEAVIMALNHGGSGRPRIMLP
                     AALHPEYKDVIRTYLAAFAVELEEYRDEPVVAAARADARTACVVAAYPDFEGRVRDLT
                     GAAEAAHAAGALFVVSADPIMLGLLRSPGSMGADIVTAEGQALGNAMNYGGPFLGMMG
                     ATQKFVRKMPGRIVGQAADHDGRRGFVLTLSAREQHIRREKAVSNICSNQGLAMLQAT
                     IYLEALGKGGLKAVARLCWDKAHYAAGLVARIPGFAVAEGEFFKEFVVRTPVTAASIV
                     EKLERRGVMPGLALSRYYPDRTHELLVCVTEMNTREQIELLARSLEEASV"
     gene            complement(1335..1718)
                     /gene="gcvH"
                     /locus_tag="PLQ29_03235"
     CDS             complement(1335..1718)
                     /gene="gcvH"
                     /locus_tag="PLQ29_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPG85690.1"
                     /translation="MSVDKNSRYRSSHEWARKDGARYLVGISDHAQEALGDIVFVELP
                     EVGKRLKQGEAFGVIESVKAASDVYMPMSGTIVAVNDAVSGDPALVNREPYAGGWLVA
                     IEASAPAEWDGLLDAAAYEAEAGKE"
     gene            complement(1790..2902)
                     /gene="gcvT"
                     /locus_tag="PLQ29_03240"
     CDS             complement(1790..2902)
                     /gene="gcvT"
                     /locus_tag="PLQ29_03240"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPG85691.1"
                     /translation="MNQTRLHDWHLAAGAKMAPFAGYDMPISYPIGSVEEHLVTRRSV
                     GLFDIDHMGQVEVTGPGADAFVSRLVSARVLDMKDGDARYSLLLSEGGTVIDDLFVYK
                     IPGAWWIVVNASNREEDLAWMNSLARAHEREFGQVHIVDRSEETYMIAVQGPRAIELL
                     DLVSDTKVSELVRFTSGNAKVLGVPCLVGRTGYTGEDGVELFFPAEDAVRLWTGLLSE
                     GEKRGIETKAIGLGARDSLRFEAGMPLHGHELSKEISPLEAGFKWACDLEKDFVGKEA
                     VLRLAESGATRKLVGLSVSGGVPREGYEVQDEGGRKVGTVVAGMFCPSVKLYAANAFV
                     EPAVAKSGTALRVIIRGKPAPAVVVKRPLYVPTYRR"
     gene            complement(3068..3484)
                     /gene="arfB"
                     /locus_tag="PLQ29_03245"
     CDS             complement(3068..3484)
                     /gene="arfB"
                     /locus_tag="PLQ29_03245"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HPG85692.1"
                     /translation="MRDDLIEASVLGAAELEYARSSGPGGQNVNKVETKVRARVDLDR
                     VDGLSEAERERVKATLASRLDADGRLFVAVDLERSRARNEAIALSRLTELIAKAAKVP
                     KKRKPTRPTRASKERRLESKRSRAGTKVGRSRPTDD"
     gene            complement(3494..4369)
                     /locus_tag="PLQ29_03250"
     CDS             complement(3494..4369)
                     /locus_tag="PLQ29_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPG85693.1"
                     /translation="MAQRKSETRGGPSKKKGAGCLVWLVMMLVTLLLFVVNWDVIKET
                     LESTGFIEAVGKSGDAQGQGDGPTSGDDQPRGDDQTRGDGSLEPTASPEAPRVVSPSP
                     AGDATAKPTGGEPSDTRSTGRPEATPSTGRPSTEAPATKPIISPMEQVKATRVAVLYF
                     IRVDDDGAISRHETKRTVGASDSPMTDALEALMAGPSADELGRGLISLIPSEARLLSA
                     QVRGSTAYLNFNEAFMYNRYGIEGYAGQLKQIVYTATNFSTVKDVQILIEGQPRDYLG
                     GDGVYIGKPLSRASF"
     gene            complement(4404..5516)
                     /locus_tag="PLQ29_03255"
     CDS             complement(4404..5516)
                     /locus_tag="PLQ29_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG85694.1"
                     /translation="MNTDESPRLPGNIAFIRIPEGMARPIGSFTVDPSIPIPVELGQG
                     APDLSDLSWEMIVAGMLRLLAYDPDNPDGAYYRAFVTAVKPELFAELSEAGILKARNG
                     DLEVAEEIFKALAGLSPEAPEPTLNLAILYEDKADALDRSGKEELADAMRSRAFEAYK
                     RLLAMEPAYPDAFFNAGLFYLKNREYGQAARLFESYAAMGDDEAKLSKAREVLSKLRV
                     RGDADAKFKEAYDFIRMGREEEGIERAMEFNRGNDGVWNGWFLVGWGHRRLGRYEDGR
                     AAFLRALELGADEVDLLNELAICEMELGMLSESRGRLERALRKEPENIKIISNLGVVA
                     RRQGRDEEAAGFFRIVLEMEPGDALARAQLDELTSA"
     gene            complement(5564..>6273)
                     /locus_tag="PLQ29_03260"
     CDS             complement(5564..>6273)
                     /locus_tag="PLQ29_03260"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPG85695.1"
                     /translation="EQWPCVIYSSSFSEVKVLAANRPALLERIAKANNLVSVRLSFKQ
                     SDTSEPVQFFISGRVAGFSPYAQSNGTLQFVSIQLTHRPPDDFVEIMGRLLEANVNSA
                     RRRDDRILLTPDVMRKIGLARKESTIMIDSVPRRCILRDLSFSGAKVIIVGLAKFLVG
                     KSCRLRVDLEDPRETVVVSGSVVRYEDVEGRKDLTAIAVRFDEATVPMSYKMHLNDYL
                     GQKKNQADDDQGGAPAP"
BASE COUNT          998 a         2055 c         2080 g         1140 t
ORIGIN      
        1 gttcatacgg aggcctcctc gagcgacctc gccaggagct cgatctgttc cctggtattc
       61 atctcggtga cgcaaacgag cagctcgtgc gtccgatccg gatagtagcg ggacagtgcc
      121 aaaccgggca tgacgccgcg gcgctcgagc ttctcgacga tggaggccgc cgtcaccggt
      181 gtcctcacga cgaattcctt gaagaactcg ccttccgcca cggcgaagcc cgggatcctg
      241 gcgacgaggc ccgccgcgta atgggccttg tcccagcaca gcctggcgac cgccttgagg
      301 ccgcccttgc cgagggcctc gaggtagatg gtagcctgga gcatggccag gccttggttg
      361 gagcagatgt tcgagacggc cttctcgcgg cggatatgct gctctcgggc cgacagcgtc
      421 agtacgaagc ccctgcggcc gtcatggtcg gcggcctggc cgacgatgcg gccgggcatc
      481 ttgcgtacga atttttgcgt agcgcccatc atgccgagga agggcccgcc gtagttcatc
      541 gcgttgccga gggcctggcc ctcggccgta acgatgtcgg cgcccataga tcccgggctc
      601 ctgagcaggc cgagcatgat cgggtcggcg ctgacgacga acagggcgcc ggcggcgtga
      661 gccgcctcgg ccgccccggt caggtcgcgg acgcggccct cgaaatcggg gtacgcggcg
      721 acgacgcagg cggtacgcgc gtcggctcgg gccgcggcga cgacaggctc gtcgcgatac
      781 tcctcgagct cgaccgcgaa ggcggccagg taggtccgta tgacgtcctt gtactccggg
      841 tgcagggcag cgggcagcat gatccgcggt cggccggagc cgccgtggtt gagcgccatg
      901 atcaccgcct cggcgagcgc ggtcgcgccg tcgtagtggg acgcattgac cgtatcgacg
      961 ccgaagagct cggcagccat cgactggtac tcgaagatgg cctgtagggt gccctgcgac
     1021 acctccggct ggtacggcgt ataggcggac aggaactcgc cgcgcgaggc gagggccggc
     1081 accgccgccg ggatgaagtg gttgtaggcc ccggcgccga ggaaccaggc gctcttggac
     1141 gcgacctcgt tcttcgaggc catggcctcg atctcgcgta gcgcctcaag ctcggacagc
     1201 ccctcgggca gccccagctt gggaaagcgc ttatcggccg gtatgtcgga gaacagctcc
     1261 tcgaccgacc tcgcgccgac ggcggcgagc atggcctcgc ggtcggcgtc ggtattcggt
     1321 atgaagggca cggcttattc cttaccggct tcggcctcgt aggcggcggc atcgaggagc
     1381 ccgtcccatt cggccggggc gctcgcctcg atagcgacga gccatccgcc ggcgtagggc
     1441 tcgcggttga ccagggccgg gtcgccggat acggcgtcgt tcacggcgac gatcgtgccg
     1501 gacatcggca tgtacacgtc gctggcggcc ttgaccgact cgatcacgcc aaaggcctcg
     1561 ccctgcttga gccgcttgcc tacctctggc aactcgacga acacgatgtc cccgagcgct
     1621 tcctgcgcgt gatcggatat accgacgagg tagcgggctc catccttacg ggcccactcg
     1681 tgggaactgc ggtaccgtga attcttgtcg acgctcatgg attcctcctt gtatcaccac
     1741 tgtaacggaa cgagtctata gaccgatagc aaccacggat gtacgggcct tagcggcggt
     1801 aggtcggcac gtataggggc cgcttgacga cgaccgccgg ggcgggtttg ccgcggatga
     1861 tcaccctgag cgctgtgccg gacttggcga cggccggctc gacgaaggcg ttagccgcgt
     1921 acagcttgac ggatgggcag aacatgccgg ccacgaccgt gccgaccttc cggccgccct
     1981 cgtcctgcac ctcgtagcct tcgcgcggta cgccgcccga gacgctcagg ccgacgagct
     2041 tgcgcgtcgc gccggactcg gccaggcgca gcaccgcttc cttgccgacg aagtccttct
     2101 cgaggtcgca ggcccacttg aagccggcct cgagcgggct gatctccttg gacagctcgt
     2161 ggccgtgaag cggcatgccg gcctcgaaac gcaggctgtc cctggcgccg agcccgatcg
     2221 ccttggtctc gataccgcgc ttctcgcctt cggacagaag cccggtccag agcctcaccg
     2281 cgtcctcggc ggggaagaac agctcgacgc cgtcctcgcc ggtatagccg gtacggccga
     2341 cgaggcaggg tacaccgagc accttggcgt tgccggaggt gaagcggacg agctcggata
     2401 ccttcgtatc ggagaccagg tcgagcagct cgatcgccct cggtccctgg acggctatca
     2461 tgtaggtttc ttcggagcgg tcgacgatat gcacctggcc gaactcgcgc tcatgcgcgc
     2521 gagcgagcga gttcatccag gcgaggtctt cctctcggtt cgaggcgttg acgacgatcc
     2581 accaggcgcc ggggatctta tagacgaaca ggtcgtcgat gacggtgccg ccctcggaga
     2641 ggagcagcga atagcgcgcg tcgccgtcct tcatatcgag cacgcgggcg ctgacgaggc
     2701 gggagacgaa ggcgtcggcg ccgggcccgg tcacctcgac ctggcccatg tggtcgatgt
     2761 cgaagagccc gacggagcgc ctggtcacca ggtgctcctc gacggagcct ataggatagc
     2821 tgatgggcat atcgtagccg gcgaacggag ccatcttggc tccggcggcg aggtgccagt
     2881 catgcaggcg agtctgattc acgattgcgt cctcctcttt cggcccgcgg cgcgcagggc
     2941 gtcggccgtt tcgaacggat caattgtagc ttgaaagcaa tccatcgaca aggtcagctc
     3001 gttaaaacga ggtttcatta ttcaaaatta taatgaagca cgactggctt cgatccggct
     3061 tcggcgctca atcgtcggtc ggccgcgagc gcccgacctt ggtgccggcc ctcgaacgct
     3121 tggactccag gcgccgctct ttcgaggctc gtgtcggcct cgtcggctta cgcttcttcg
     3181 gcaccttggc ggccttggcg ataagctcgg tcagcctcga cagggcgatc gcctcgttcc
     3241 gagccctgga gcgctcgagg tccacggcga cgaagagtcg gccgtcggcg tcgagcctgg
     3301 aggcgagcgt cgccttgacg cgctcgcgct cggcctccga gaggccgtcg acgcgatcca
     3361 ggtcgacgcg ggcgcgcacc ttcgtctcca ccttgttgac attctggccg cccggcccgg
     3421 acgacctggc gtactcgagc tcggcggcgc ccaacactga ggcttctatc aggtcgtcgc
     3481 gcatggcgcc accctagaag gacgcccgcg acagcggttt gccgatgtac acgccgtcgc
     3541 cgccgagata gtcgcgcggc tgcccctcga tgagtatctg gacgtccttg accgtggaga
     3601 aattcgtggc agtatagacg atctgcttga gctggcccgc gtagccctcg atgccatagc
     3661 ggttgtacat gaatgcctcg ttaaaattca ggtaggccgt cgagcctctg acctgggccg
     3721 acaggagcct cgcttccgag ggtatcaggc tgatcagccc tcggcccagc tcgtcggcgc
     3781 tcggaccggc catcagggct tcaagggcgt cggtcatcgg cgaatcggag gctccgacgg
     3841 tgcgcttggt ctcgtggcgg ctgatggcgc cgtcatcgtc gacgcgtata aaatacagca
     3901 ccgccaccct ggtcgccttc acctgctcca tcgggctgat gatcggcttg gttgccggag
     3961 cctcggtcga ggggcgtcct gtcgaggggg tggcttcggg gcgtccagtc gatcgcgtat
     4021 cggacggctc gccgccggtc ggctttgcgg tcgcgtcgcc ggctggcgaa gggctgacga
     4081 ccctcggcgc ctcggggctg gccgtcggct cgagactacc gtctccgcgg gtctggtcgt
     4141 cgccccgggg ctggtcgtcc ccgctggtcg gaccgtcgcc ttggccctgg gcgtcaccgg
     4201 acttgccgac ggcctcgatg aagccggtcg actcgagcgt ctccttgatc acgtcccagt
     4261 tgacgacgaa caacaggagc gtaacgagca tcatgacgag ccagacgagg cagcccgcgc
     4321 ccttcttctt gcttggacct ccgcgggtct ccgacttgcg ttgagccatt atgggtcgat
     4381 actagccgcg ctccgcggcg gggtcaagcg gaggtcaatt cgtcgagctg agccctcgcg
     4441 agcgcatcgc ccggctccat ctcgagcacg atcctgaaga acccggccgc ctcctcgtcc
     4501 ctaccctgcc tgcgggccac gacgccgagg ttcgagatga tcttgatatt ctcgggctcc
     4561 ttgcgtagcg cccgctcgag cctgccccgg ctctcggaca gcatgccgag ctccatctcg
     4621 cagatggcca attcgttcag gaggtcgacc tcgtcggcgc ctagctcgag ggcgcggagg
     4681 aacgcggcgc ggccatcctc gtagcgtccg aggcgacggt gtccccagcc gacgaggaac
     4741 cagccgttcc agacaccgtc gttgccgcgg ttgaactcca tggctcgctc gatgccttcc
     4801 tcttcccggc ccatgcgtat gaaatcatag gcttccttga atttcgcgtc ggcgtcgccg
     4861 cggaccctga gcttcgacag cacctcgcgg gccttggaca gcttggcctc gtcatccccc
     4921 atggccgcgt acgactcgaa cagcctggcg gcctggccgt actcccggtt cttgaggtag
     4981 aagaggccgg cgttgaagaa tgcgtcggga tacgccggct ccatggccag gagccgttta
     5041 taggcctcga aggccctgga gcgcatcgcg tctgccagtt cctccttacc ggaacgatcg
     5101 agggcgtcgg ccttgtcctc gtagaggatg gccaggttca gagtcggctc gggcgcctcg
     5161 ggtgagaggc cggcgagcgc cttgaagatc tcctcggcca cctcgaggtc gccgttcctg
     5221 gccttgagaa tgccggcctc ggacagctcg gcgaatagct ccggcttcac ggccgtcacg
     5281 aaggcgcgat aataggctcc gtcggggttg tccgggtcgt acgcgagcag gcgtagcatg
     5341 ccggccacga tcatctccca gctcaggtcg gataggtcgg gcgcgccttg gcctagctcc
     5401 acgggtatcg gtatggacgg gtcgaccgtg aacgagccta tgggcctggc catgccctcg
     5461 ggtatcctga tgaaggctat gttgcccgga aggcgcggcg attcgtcggt attcatcgtt
     5521 tctccgtatt cggccccctc ggggccagtc cgctgggtag ggatcaaggg gcgggagcgc
     5581 cgccctgatc gtcgtcggcc tggttcttct tctgtcccag gtagtcgttc aggtgcatct
     5641 tgtaggacat gggcaccgtc gcctcatcga agcgtacggc gatggcggtc agatcctttc
     5701 ggccctcgac gtcctcgtag cggaccacgg agccggacac gacgacggtc tcgcgcggat
     5761 cctcgaggtc gacgcgcagc ctgcagctct tgccgacgag gaacttggcc agcccgacga
     5821 tgatcacctt ggcgccggag aacgacaggt ctcgcagtat gcaccgacgc ggtaccgagt
     5881 cgatcatgat cgtcgactcc ttgcgggcca ggcctatctt gcgcatcacg tcgggcgtca
     5941 ggagtatgcg atcgtcgcgg cggcgcgcgg aattgacgtt ggcctcgagc agtcggccca
     6001 tgatctccac gaaatcgtcc ggcgggcggt gcgtcagctg tatcgagacg aactgcagcg
     6061 taccgttcga ctgagcgtac ggcgagaagc cggccactcg acccgatatg aaaaactgga
     6121 ccggctcgga ggtgtccgac tgcttgaacg acagccggac actgaccaaa ttgttggcct
     6181 tggcgatgcg ctccagcaag gccggcctgt tggccgccag caccttgact tcgctgaacg
     6241 agctcgagta gatgacgcac ggccactgct ccc
//
LOCUS       DAOPHV010000077         6258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_16300_length_6258_cov_4.558278, whole genome shotgun sequence.
ACCESSION   DAOPHV010000077 DAOPHV010000000
VERSION     DAOPHV010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16300_length_6258_cov_4.558278"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1708)
                     /locus_tag="PLQ29_03265"
     CDS             complement(86..1708)
                     /locus_tag="PLQ29_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPG85696.1"
                     /translation="MPDFFETDDIVKSYDGAIVRRIFAYVKPYKGLLAAATLTLALST
                     IGELVTPVLVQRSIDEALVVSWVSLDTELASGPRLRGIDVPSDAPRSGGRLYLRSGEL
                     ASLSSRVREDLERDGSLDPGPYYLAQVVDAEALPDVVAADLGVPGGPVLADGWLVAPS
                     AYVASLSAEDARALRSEDRRVVRLYGWTLLAVLIVVLFSTFAQAWTTSLIGQNVMKDL
                     RMDLFKRTSRRSIASLSRQPVGRLVTRLTSDVETINEFFTNVVVSFIKDLSLMAGSLV
                     VLVAMSPRLAFIAIATLPPVLVATGVSRRKARDAFRRQRTWLSKVNAFIAEHIAGVAI
                     VKLFGRERASSEAFARDDAELMRANLGEMYVFAVFRPVIEFLSSVSIAVILWFGAGFH
                     GSALITLGTLIAFVNLIRMFYSPLQDISEKYTMLQSAMAGGERVFKLLDDDERIPDEP
                     TASLTVPVRGRIEFDHVWFAYKGEDWVLRDLSFTVEPGETLAIVGYTGAGKSTVGSLL
                     ARLWTLSAARYGWTACRYGSCPSTRYARRYGR"
     gene            complement(1701..3470)
                     /locus_tag="PLQ29_03270"
     CDS             complement(1701..3470)
                     /locus_tag="PLQ29_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG85697.1"
                     /translation="MLKEFKSLLPYLRRYKWHYALGLLSLVVVDAAQLLLPQYLRKAV
                     DSIAADGPRSIVAAIGASMVATAAAIAVGRFLWRYFIHGASRRIETELRDRLFARLMS
                     LPGSYFGEHAAGDIMARATNDMQAIRKATGMAFVAFVDGAFMSVAILAVMIAQNPGTT
                     LLTIAPLPLISVLIVVFGSMVGKRFERVQALYSGMSSVAQETFQGIRVVQSFVKEDEF
                     AKRFSEANDRYRAASMSLVMIYGFFFPLISFLAGLTTLVLMVAGGAAVIENRMTPGAL
                     AAMLSYLEMLIWPMIGAGFTVNMLQQGAASLKRVNEVLHSEPEPRFAPGALQVAVAAP
                     RGGVAFDGLTVLYPGSSRPALDGVELAIGEGRVLGVLGRVGSGKSTLVKTLPRIVEPP
                     RGAVRFGGVDVGSYPLEALRSAIGFVPQDSFLFSDSLRANICFADPDLSASRFEEVVA
                     IAALERDEKLFARGWDTVVGERGLTLSGGQKQRVAIARALARDPELLVLDDSLSAVDA
                     ETEEAIVEALLADRAGKTTIIISNRVSTLRRADLVAVLDGGRLAQFGSPAELAGADGF
                     YAEIAALQALSAEPEARGERADA"
     gene            3606..4490
                     /locus_tag="PLQ29_03275"
     CDS             3606..4490
                     /locus_tag="PLQ29_03275"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HPG85698.1"
                     /translation="MKKFMRFAMAVALALQGCALLDAGDYASRAFMRESDPALAKDAF
                     PTMIKAAEAIQLADPDDQGAATLVASLYVMYANAFLDGEAFLLPDEAFEERLGLARRA
                     NALYLRAAALLAPFVEARAPGALGLAVVTEDPSLARLGKKDVPTLYWAAAAILAAFAS
                     DPMDFDNAARVSGALALFERARTVEPDWNGGALHELAISVYGSLPTELGGDRAKARAA
                     FELAKAASSASSPGAFVAYASSVCAPEGDADGFAQALETALGLPDRPESALMDAIARR
                     KARRLLDDKDLYFYLGDI"
     gene            4487..5473
                     /gene="dctP"
                     /locus_tag="PLQ29_03280"
     CDS             4487..5473
                     /gene="dctP"
                     /locus_tag="PLQ29_03280"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPG85699.1"
                     /translation="MRHLKIVLVALSLVAGLSAASAQAVTLQIGSNAPVNSPWDLGVK
                     RIAAEWSRISEGRVKVVFPKSVANSSMEDLIQKLKFTLDGAVLDTSGLGVIDKDVFFL
                     SMPSVIRDDAEFDAAIAAALPILRKRLGDRYEIVAVAKAGWIRFFSNRPLRSPEDLRN
                     LRMGVNRNMDALTKVMQSLGVRTVQADSASTLLQFNSGAMDAMYSSPLFVGALWSQYR
                     RVVTHMTGFKVAPFFGAIIVNRRSWDRVPEALKPALREAAESICRDIGNEAARLEDEG
                     IAAMTRGGLQVPPYAQADAAAWNELFSSTVEGFMADWYSREFTAAVYEAIGR"
     gene            5475..>6258
                     /locus_tag="PLQ29_03285"
     CDS             5475..>6258
                     /locus_tag="PLQ29_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85700.1"
                     /translation="MPTDETDAGRLGPLLNVAMACAALGAAALTLVPVLNLILARLFW
                     RGLPEAGPLTEHALIALAFFAAAVAGIRGTHLSLGSAGAAKGRLGMTIAGLRSAFSTA
                     VDTTLFFAGLSLVLIGFEPADRAFGVPIAVFASPLAVGYLLMTIADLARPKGRARLAS
                     FAGLAVGVFLSASPIANVLSAFGVYLEPVDGAVAAVGALCGAAAIPLIVLLVALAFRG
                     LPLYAVIAGSAILLYLGAGSAVELMPSEAYSLFMNDSMPAIPL"
BASE COUNT         1046 a         2109 c         2084 g         1019 t
ORIGIN      
        1 gctctccgga tatcgtccgc tccgaggccg agccccaggt cgatattgcc cgcgacggtg
       61 ccggagaaca ggaacacgtc ctgcatcacc ggccgtatcg acgagcgtag cgcgtcgagg
      121 ggcagctccc gtatcggcac gccgtccagc cgtatctcgc cgcgctgagg gtccagagcc
      181 tggccaggag cgagccgacg gtgctcttgc cggccccggt atagccgacg atcgcgaggg
      241 tctcgcccgg ctccaccgtg aacgacaggt cgcgaagcac ccagtcctcg cccttgtacg
      301 cgaaccagac atggtcgaac tcgatgcgcc cccgaaccgg gacggtcagg ctcgccgtcg
      361 gctcgtcggg gatgcgctcg tcgtcgtcca gcagcttgaa gacccgctct ccgccggcca
      421 tcgcgctctg gagcatcgtg tacttctcgg agatgtcctg gagcggcgag tagaacatcc
      481 tgatcaggtt gacgaaggcg atgagcgtgc cgagcgtgat cagggcggaa ccgtggaagc
      541 cggcgccgaa ccacaggatc acggctatcg acaccgacga taggaactcg atgaccggcc
      601 tgaacaccgc gaacacgtac atctcgccga ggttcgcccg catcagctca gcgtcgtcgc
      661 gcgcgaaggc ctcggaagag gcccgctcgc gaccgaacag cttgacgatc gcgacgccgg
      721 ctatgtgctc ggctatgaag gcgttcacct tggacagcca ggtccgctgc ctgcggaacg
      781 cgtcccgggc cttgcgcctg gagacgccgg tcgcgacgag gaccggcggc agggtggcga
      841 tcgctatgaa agccaggcgc gggctcatcg cgaccagcac gacgagggat ccggccatca
      901 ggctcaggtc cttgatgaac gatacgacga cgttcgtgaa gaattcgttg atcgtctcga
      961 cgtcgctcgt cagcctcgtg acgagccggc ctaccggttg cctggacagg ctggctatgg
     1021 agcggcggct cgtccgcttg aacaggtcca tgcgcaggtc cttcatcacg ttctggccta
     1081 tgaggctcgt ggtccaagcc tgggcgaagg tcgagaagag gacgacgatc aggacggcga
     1141 ggagggtcca gccgtacagg cggacgaccc gccggtcctc ggagcgcaac gctcgggcgt
     1201 cctccgctga tagcgacgcg acataggccg acggcgcgac gagccagccg tcggcgagga
     1261 ccgggccgcc cgggactcca aggtcggcgg ctaccacgtc cggcaaggcc tcggcgtcta
     1321 cgacctgcgc caggtagtac gggcccgggt cgagcgagcc gtcgcgttcg aggtcctctc
     1381 gcactcggga cgatagcgag gccagctcgc cggagcgcag gtacagcctg ccgccggatc
     1441 gcggcgcgtc ggacggtacg tctatccctc tcagcctggg gcccgaggct agctcggtgt
     1501 ccagagagac ccacgaaacg acgagggcct cgtcgatgga gcgctggacg agtaccggcg
     1561 tgacgagctc gccgatggtg gacagggcca gtgtcagcgt agccgccgcc aggaggccct
     1621 tgtatggctt gacgtaggcg aagatgcgcc tgactatggc tccgtcgtag ctcttgacga
     1681 tgtcgtcggt ctcgaagaaa tcaggcatcg gctcgctccc ctcgggcctc gggctcggcg
     1741 ctcagggcct ggagcgccgc tatctcggcg tagaagccgt ccgccccggc cagctcggct
     1801 ggcgaaccga actgggcgag ccggccgccg tcgagcacgg ccaccaggtc cgcccgcctg
     1861 agcgtcgaga cccggttgga tatgatgatg gtcgtcttgc cggccctgtc ggccagcaac
     1921 gcctcgacga tggcttcctc ggtctcggcg tccaccgccg acagcgagtc gtcgagcacg
     1981 agcagctccg ggtcgcgcgc caaagccctc gctatggcca cccgctgctt ctggccgccc
     2041 gagagggtca ggccccgctc gcccacgacc gtgtcccagc cgcgggcgaa tagcttctcg
     2101 tcgcgctcga gcgccgcgat ggccacgacc tcttcgaaac gcgaggccga caggtcgggg
     2161 tcggcgaaac atatattggc gcgcaggctg tcggagaaca ggaagctatc ctggggcacg
     2221 aagcctatgg ccgagcgcaa cgcctcgagc gggtacgagc cgacatcgac gccgccgaag
     2281 cggaccgccc cgcgaggagg ctcgacgatg cgcggcaacg tcttgaccag ggtcgacttg
     2341 ccgctgccca cccggccgag cacgccgagg acccggccct cgcctatggc cagctcgacg
     2401 ccgtcgaggg cggggcgcga gctaccggga tacaggacgg tcagcccatc gaacgccacg
     2461 ccgcccctgg gcgcggcgac cgcgacctgg agggcgcctg gcgcgaatct gggctccggc
     2521 tcgctatgca gcacctcgtt gacccgtttg aggctggcgg ctccctgctg gagcatgttc
     2581 acggtaaagc cggcgcctat catcggccag atgagcatct cgaggtacga cagcatcgcc
     2641 gcgagggccc ctggcgtcat gcggttctcg atgacggcgg cgccgccggc gaccatcagc
     2701 acgagggtcg tcagtccggc caggaacgaa atgagcggga agaagaagcc gtagatcatc
     2761 acaaggctca tcgaggccgc gcggtagcgg tcgttcgcct cggagaagcg cttggcgaat
     2821 tcgtcctcct tgacgaagga ctggaccacg cgtatgccct ggaacgtctc ctgggccacc
     2881 gaactcatgc ccgagtacag ggcttggacg cgctcgaagc gcttgccgac catggagccg
     2941 aagacgacga tgagcacgga tatcagcggc agaggcgcga tggtcaacag ggtcgtgccc
     3001 gggttctggg ctatcatcac cgcgagtatg gcgacagaca tgaaggcccc gtcaacgaag
     3061 gcgacgaaag ccataccggt ggccttgcgt atcgcctgca tgtcgttcgt ggccctggcc
     3121 atgatgtcgc ccgcggcgtg ctcgccgaag tacgagccag gtaggctcat gagcctggcg
     3181 aacaatcggt cgcgcagttc cgtctcgatg cggcgcgagg cgccgtgtat gaaatagcgc
     3241 cataggaagc gcccgacggc gatggccgcg gccgtggcga ccatggacgc gccgatggcg
     3301 gcgacgatcg atcgcggtcc gtcggccgct atcgagtcga cggccttacg caggtactgg
     3361 ggaaggagca actgcgcggc gtccacgacg acgaggctga ggaggccgag cgcgtagtgc
     3421 cacttgtaac gcctcaggta cgggagcagg ctcttgaatt cctttagcat cggagaatgg
     3481 tataccgtat tccgtcaagt ttcaacgtat cacgtttctc cggcgcaagc gatatccaca
     3541 ggatcctaac ccgggctgaa gcgaacgcgg ttgacagcca gacgacgagc ataaatacta
     3601 tccgtatgaa gaaattcatg cgtttcgcca tggctgtcgc gcttgccctg cagggttgcg
     3661 cccttctcga tgccggcgac tacgcctccc gcgccttcat gcgcgagagc gacccggccc
     3721 tagccaagga cgcctttccg acgatgatca aggcggcgga ggcgatccaa ctcgccgatc
     3781 ccgatgacca aggggcagcg acgctcgtgg cgtcgctcta cgtgatgtac gccaacgcct
     3841 tcctcgacgg cgaggcgttc ctattgcccg acgaggcctt cgaagagcgc ctggggctcg
     3901 ccaggcgggc caacgcgctg tacctgcgag cggcggccct gctagcgccg ttcgtcgagg
     3961 ccagggcgcc cggcgccctt ggtctggccg tcgtcactga ggacccgtcc ctggcccgcc
     4021 ttggtaaaaa agacgtaccg acgctgtatt gggccgcggc cgctatcctg gccgccttcg
     4081 cgtccgaccc gatggacttc gacaacgccg ccagagtctc gggcgcgctg gccctgttcg
     4141 agcgggcccg aaccgtcgag cctgactgga atggcggagc gttgcacgag ctagccatct
     4201 ctgtgtacgg ttccctgccc accgagctcg gcggggaccg cgccaaggcc agggcggcct
     4261 tcgagctggc aaaggcagcg tcgagcgcct cgtcgcctgg ggctttcgtg gcgtacgcgt
     4321 cgtccgtctg cgccccggag ggagacgcgg acggcttcgc tcaggcgctc gagacggcgc
     4381 tcggcctgcc ggatcggccg gagtccgccc tcatggacgc gatcgccagg cgtaaggcgc
     4441 gaaggctgct cgacgacaag gatctatatt tctacctggg ggatatatga ggcatcttaa
     4501 aatcgtcctc gtcgcgctgt cgctcgtcgc cgggctgtcc gcggcctcgg ctcaggccgt
     4561 cacgttgcag ataggctcga acgcgccggt caacagcccc tgggacctcg gcgtcaagcg
     4621 gatagccgcc gagtggtcga gaatttcgga gggccgcgtc aaggtggtat ttcccaagag
     4681 cgtcgccaat tcgagcatgg aggacctgat ccagaagctc aaattcacgc tcgacggggc
     4741 cgtcctcgac acgagcgggc tcggcgtcat cgacaaggac gtgttcttcc tatcgatgcc
     4801 ctcggtcata cgcgacgacg cggaattcga cgcggcgatc gccgccgccc tacccatcct
     4861 gaggaagcgc cttggcgatc gctacgagat cgtggcggtc gccaaggccg gctggatacg
     4921 cttcttctcg aaccggccgc tacgctcgcc cgaggacctg cgcaacctgc gcatgggcgt
     4981 caaccgtaac atggacgcgc tgaccaaggt gatgcaatct ctgggcgtgc gcaccgtgca
     5041 ggcggactcg gcctcgacgc tactgcaatt caactccggc gccatggacg cgatgtactc
     5101 gagcccgctg ttcgtcggcg cgctctggtc gcagtaccgg cgcgtcgtca cgcacatgac
     5161 cggtttcaag gtcgcaccct tcttcggcgc catcatcgtc aaccgtaggt cctgggacag
     5221 ggttcccgaa gcgctcaagc cggccctgcg cgaagccgcc gagagtatct gccgcgatat
     5281 agggaacgag gcggccaggc tcgaagacga gggcatagcg gcgatgacgc gaggcggcct
     5341 acaggtgccg ccctacgcgc aggcggacgc ggccgcctgg aacgagctgt tctcgtcgac
     5401 cgtggaaggc ttcatggcgg attggtacag ccgggagttc accgccgccg tctacgaggc
     5461 cataggccgt tagcatgccg acggacgaaa cggacgccgg gcgtctcggc ccgctcctga
     5521 acgtggctat ggcctgcgcc gccctaggcg cggccgcgct gacgctggtc cccgtattga
     5581 acctcatcct ggcccgcctc ttctggcgag gcctgcccga agcgggtccg ctgaccgagc
     5641 acgcgctcat agcgctcgcg ttcttcgcgg ctgccgtcgc cggtatccgg gggacgcacc
     5701 tatcgctcgg ctccgccggc gccgcgaagg gccgactcgg gatgacgata gccgggctca
     5761 ggtcggcctt ctccacggcc gtcgatacga cgctgttctt cgccggcctg tcgctcgtac
     5821 tgatcggctt cgagccggcc gatcgggcct tcggcgtccc gatcgccgtc ttcgcctcgc
     5881 cgctggccgt gggatatctc ctcatgacga tcgccgacct cgccaggccc aagggaaggg
     5941 cccgcctggc ctcgttcgcc gggctcgccg tcggcgtctt cctgtccgcc tcgccgatag
     6001 cgaacgtcct gtccgccttc ggtgtctacc tcgagcccgt cgacggcgcg gtagccgccg
     6061 taggcgcgct ctgcggcgcg gccgccatcc ccctcatcgt cctgctcgtc gcgctggcgt
     6121 tcaggggctt gcccctctac gccgtcatcg ccggatcggc catactcctg tacctcggtg
     6181 ctggctcggc cgtcgagctg atgccgagcg aggcctacag cctgttcatg aacgacagca
     6241 tgccggccat cccgctgt
//
LOCUS       DAOPHV010000078         6240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_16373_length_6240_cov_3.950202, whole genome shotgun sequence.
ACCESSION   DAOPHV010000078 DAOPHV010000000
VERSION     DAOPHV010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16373_length_6240_cov_3.950202"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLQ29_03290"
     CDS             complement(<1..564)
                     /locus_tag="PLQ29_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG85701.1"
                     /translation="MKEKKEGFRNYMGLTFLIGFGFFTMGLMDPLYDTYLPAFLGRYI
                     SSKTLVGAVMTLDNVLQLFLIPIVAVWSDRTRTRIGRRMPFILVMLPASAVFFGLIPW
                     SAGVSLGALITMIFLLNMFKTSVRGPVVALMPDTIPGEYRSEANGVINTMGGIGTIIG
                     TLALARLMDLDVVLPIIGRTKDMLPFPL"
     gene            complement(561..1844)
                     /locus_tag="PLQ29_03295"
     CDS             complement(561..1844)
                     /locus_tag="PLQ29_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HPG85702.1"
                     /translation="MHDPDSLLRLPRSREGQELLARWYGGSRVSDQRDRWERLAESTF
                     ALFDPREATFVSSPGRTELGGNHTDHNDGRVLCASVHLDALACVAPRGDLRVELRSEG
                     WDAPFELDLGSLEPVPAERGRPEALIRGVAAAIVRRGGSIGGFSGRMCSSVPAGSGLS
                     SSASVELLFGQIQNALYNRNRLEPVLLAMAGKEAENRHFGKPCGLMDQMACALGGVSA
                     IDFGVPEQPTWSRVSFDFEKAGYAIAIVNAGGSHADLTDDYASIPTEMRAVAELYGLP
                     SLRRLTMDKVIRKAAEIRERLGDRALLRAMHFVAENERAGDMAAALEAGKMKKYLKLV
                     RRSGDSSWRLLQNVTPAGALRDQSLAAAIELSNAFVGKEGASRVHGGGFAGTIQAYVP
                     IDRVPEYSEYMDARFGAGATTIVHIRPDGAGEANV"
     gene            complement(2007..2201)
                     /locus_tag="PLQ29_03300"
     CDS             complement(2007..2201)
                     /locus_tag="PLQ29_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85703.1"
                     /translation="MKTFAIKSTDRAPSVLELVGEIEDGYVVRIVRHRDDWEDVSEEF
                     MTRELFDTCMRTGYIYEMSA"
     gene            2413..4350
                     /locus_tag="PLQ29_03305"
     CDS             2413..4350
                     /locus_tag="PLQ29_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPG85704.1"
                     /translation="MPSLTFSDFGFSAPVLEALARKGFEEPTPIQSLAIPVLLSGSSH
                     LVARARTGTGKTAAFGLPLVERLSTPGPKVRALVLVPTRELAVQVSGEIASFAPGPAP
                     RIATVYGGASMGEQLRRLSRGVDIVVGTPGRVLDHLDRKTLDLSALEWLVLDEADEML
                     DMGFIEDIEKVMDASNPERRVVLFSATMPHPIVRIVRDRLGAFETVEDSTNPVQAGLT
                     DQIWLEVRSRDKLEALCRIVDAEDEFYGLVFCHTKIETDSVARALSERGYAAEALHGD
                     VSQDMRERTLARFRDKKTLILVATDVAARGIDVERLTHVVNFNLPFDPESYTHRIGRT
                     GRAGNKGTAITFVTPEEYRRLFALRRASGDGLHKGSVPTVGDVIEAKRSRILAKAAGA
                     ARETLGLELKRDDEADDAAAVDPAVVGAADAVVAPAVVAVPVAAPAAEDGAAEDGAAE
                     DGAAPACEPTVEPGAAKPKRKAKAKADPAVASFYTLADELLAMLPPREALASLAAAAY
                     SGDLDPRRYGEVRDVSVDSAATARLYVGIGKQDGAWPKDVAAVVKRLTGLPDRLVDDI
                     EVLERFSFVSVPFEAAEKAIAEARKQRGAPPVRMATPKSGPGGPGGASGGKRGYGPRP
                     SYGSKGGYDRPKPYKKPRPRD"
     gene            complement(4357..5166)
                     /locus_tag="PLQ29_03310"
     CDS             complement(4357..5166)
                     /locus_tag="PLQ29_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPG85705.1"
                     /translation="MESETSQPPEDQGLERNGLFGKKVFFLNPPGVIGDVANALAEAE
                     FEVYSTRDHAKLARFLRKEPECLTFVNIDEGDDESVWRAWIKSIKGEEASARAAFGII
                     TMLDDEDKRSYYLMDLGVECGFVVVKIGVAKTTEILLRMLEANEARGRRRYVRAACPP
                     DSTELSCPTDEGLLRGWIKDLSSVGMSVAFAGGATPRQGSRLRDMQLNLKGVRVILNG
                     VVIGSHDAPGIGVISVIMFEPASVNDDKRAKLRAFIRRTLQATMDKALSLA"
     gene            complement(5214..5744)
                     /locus_tag="PLQ29_03315"
     CDS             complement(5214..5744)
                     /locus_tag="PLQ29_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPG85706.1"
                     /translation="MLHAIGDAIPRVARSAFVAWNAEVAGDATIGEDSSVWFGVTVRA
                     DMAPIRIGSGTNIQDGSVVHVADETPCVVGDRVTVGHGVILHGCEVGDDCLVGMGATL
                     LNGSRIGPGSIVAAGALVTQGKAFPPRSMIMGSPAKAVRTISDEELERIRRNGLEYVA
                     MARETASYRRIDGDDD"
     gene            5875..>6240
                     /locus_tag="PLQ29_03320"
     CDS             5875..>6240
                     /locus_tag="PLQ29_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HPG85707.1"
                     /translation="MAPELDDVHEIIQIDSELNKIRDYDILLERVLLEARRVVHADAG
                     SIYVKEGDVISIKFAHNDTKQKSLPPGEKQAYPHFSIPINDKTISGYVAMTGEAVNIP
                     DVYNLPPGLPFSYNTAYDAK"
BASE COUNT         1002 a         2182 c         2023 g         1033 t
ORIGIN      
        1 cagcgggaag ggtagcatat ccttggtgcg gccgatgatg ggtagcacga cgtcgaggtc
       61 catcaggcgc gccagggcca gcgtgccgat gatcgtgcct atgccgccca tcgtgttgat
      121 cacgccgttg gcctcggagc ggtattctcc ggggatggtg tcgggcatca aggcgacgac
      181 cgggccgcgg acgctcgtct tgaacatgtt gaggaggaag atcatcgtga tgagcgcgcc
      241 gagcgatacg ccggcggacc acggtatcag gccaaagaac accgccgagg ccggcaacat
      301 cacgaggatg aagggcatgc gccggcctat cctggtccgc gtccggtcgc tccataccgc
      361 gactatcggg atcaggaaca gctgcaggac gttgtcgagc gtcatcaccg cgccgacgag
      421 cgtcttggac gagatgtacc tgccgaggaa ggccggcagg taggtatcgt acagcggatc
      481 catcaggccc atcgtgaaga acccgaagcc gatcaggaag gtcaggccca tgtagttcct
      541 gaagccctct ttcttttctt tcatacgttc gcctctccgg ccccgtcggg cctgatatga
      601 acaatggtgg tggccccggc gccgaagcgc gcgtccatgt actcggagta ctcggggacg
      661 cgatcgatcg gcacgtaggc ctggatggtg ccggcgaagc cgcccccgtg caccctggac
      721 gcgccttcct tgccgacgaa ggcattcgac agctctatgg ccgccgcgag cgactggtcg
      781 cgcaacgccc cggccggcgt cacgttctgg aggagcctcc aggacgagtc gccggagcgc
      841 ctgacgagct tcaggtactt cttcatcttg ccggcctcga gggcggccgc catgtcgccg
      901 gcgcgctcgt tctcggcgac gaagtgcatc gctcgcagca gggcccggtc gccgagacgc
      961 tcgcgtatct cggccgcctt cctgattacc ttgtccatcg tcagcctgcg caggctcggc
     1021 aggccgtaca gctcggccac ggcgcgcatc tcggtcggta tgctcgcgta gtcgtcagtc
     1081 aggtcggcgt ggctcccccc ggcgttgacg atggctatcg cgtagcccgc cttctcgaag
     1141 tcgaaggaca ccctcgacca ggtcggctgc tcgggtacac cgaagtctat ggccgagacg
     1201 ccgccgagcg cgcaggccat ctggtccatc agcccgcagg gcttgccgaa gtgacggttc
     1261 tccgcctcct tgccggccat cgccagcagg acgggctcga ggcggttccg gttatagagg
     1321 gcgttctgga tctggccgaa cagcagctcc accgacgccg agctcgagag gcccgagccg
     1381 gccggcaccg acgagcacat ccgcccggag aaaccgccta tcgagccgcc gcgcctgacg
     1441 atggcggcgg ccaccccgcg gatcaatgcc tcgggcctgc ctctctcggc cggtacgggc
     1501 tcgagcgagc ccaggtccag ctcgaacggg gcgtcccagc cctcggagcg cagctcgacg
     1561 cgcaggtcgc cgcgcggcgc cacgcaggcc agggcgtcga ggtggaccga cgcgcagagc
     1621 acgcggccgt cgttatggtc ggtatggttg ccgcctagct cggtgcggcc cgggctcgag
     1681 acgaaggtcg cctctcgcgg gtcgaagagg gcgaacgtcg actcggcgag ccgctcccat
     1741 cggtcccgct ggtccgagac ccgcgagcct ccgtaccacc tggccagcag ttcctggccc
     1801 tcgcgcgatc gcggtagccg taatagcgaa tcggggtcgt gcacgttgtc tccctgggcc
     1861 ggcgctgcgc gtccggcgcg ttggtctaga gtagcggaca gcgatagcac gatagcacgg
     1921 tttcgcgcct tgggaaagcg aaggccgccc ctcggggcgg cctcctacgc tggtcgttta
     1981 catggttcga ggtgttttac ggctcgctag gcggacattt catagatata gccggtgcgc
     2041 atgcaggtat cgaacagctc ccgggtcatg aactcctcgc tgacgtcttc ccagtcgtct
     2101 cgatgcctga cgatgcgcac gacgtacccg tcttcgatct cgccgacgag ttcgagtacc
     2161 gaaggcgcgc gatcggtgct ctttatggcg aatgtcttca tcttcatcct ccgatcgacg
     2221 ctgttgcgtc cttacctata atgtcggcgg gccgcgcgcg gcggtttacg cctatttacg
     2281 tgcccggcgg tctggactat cgatccccga aacgctatag tcgtaccatc ccgcgggacg
     2341 ggcttccact cgtcgattcg cacctcgccg aagattcgcc cacgagacca tacgttccct
     2401 ataaggacac cgatgccttc tctaaccttc tccgatttcg gattctccgc gcccgtgctc
     2461 gaggctctcg cccgcaaggg cttcgaggag cccaccccga tccagtcgct ggccatcccg
     2521 gttctgctct cgggctcgtc ccatctcgtt gcccgcgcca ggaccggcac cggtaagacg
     2581 gccgccttcg gccttccgct cgtggagcgc ctgtcgacgc ccggacccaa ggtccgcgcc
     2641 ctcgtcctcg tgccgacccg cgagctggcc gtccaggtct ccggcgagat cgccagcttc
     2701 gctcccggtc cggccccgcg catcgccacg gtctacggcg gcgcctccat gggcgagcag
     2761 ctcaggcgcc tatcccgcgg cgtcgacatc gtcgtcggca cgcccggccg cgtcctggat
     2821 catctcgacc gtaagaccct cgacctgtcg gcgctcgagt ggctcgtact cgacgaggcc
     2881 gacgagatgc tcgacatggg cttcatcgag gatatcgaga aggtgatgga cgcgtcgaac
     2941 cccgagcgcc gcgtcgtgtt gttctccgcc acgatgcccc atcctatcgt gcgcatcgtg
     3001 cgcgaccggc tcggcgcctt cgagacggtc gaagactcga ccaacccagt ccaggccggc
     3061 ctgaccgacc agatctggct cgaggtccgc tcccgcgaca agctcgaggc cctctgccgt
     3121 atcgtcgacg ccgaggacga gttctacggc ctggtgttct gccataccaa gatagagacc
     3181 gattcggtcg cccgcgccct gtcggagcgc ggctacgcgg ccgaggccct gcacggcgac
     3241 gtgtcccagg acatgcgcga gcgcaccctc gcccgtttcc gcgataagaa gacgctgatc
     3301 ctggtggcga ccgacgtggc cgcgcgaggc atcgacgtcg agcggctgac ccacgtggtc
     3361 aacttcaacc tgcccttcga cccggagtcg tatacgcacc gcatcggccg cacgggccgg
     3421 gccggcaaca agggcaccgc cataaccttc gtgacgccgg aggagtaccg tcgcctgttc
     3481 gccctgaggc gcgcctcggg cgacggcctc cacaagggta gcgtgccgac cgtcggcgac
     3541 gtgatcgagg ccaagcgctc gcgcatcctg gccaaggcgg ccggagcggc ccgcgagacc
     3601 ctcgggctcg aactgaagcg cgacgacgag gccgatgacg ccgcggcggt cgatcccgcc
     3661 gtcgtcgggg ccgccgacgc ggtcgtcgct ccagcggtcg tcgccgtacc ggtagccgct
     3721 ccggcggccg aggacggagc cgccgaggac ggtgccgccg aggacggagc cgcccccgcc
     3781 tgcgaaccga ccgtcgagcc aggcgccgcc aagcccaaac gcaaggccaa ggccaaggcc
     3841 gacccagccg tcgcctcgtt ctatacgctg gccgacgagc tgctcgcgat gttgccgccc
     3901 agggaggcgc tcgcctcgct cgccgcggcg gcctactccg gcgacctcga cccgaggcgc
     3961 tacggcgagg tccgcgacgt gtcggtcgac tcggccgcga cggcccgcct gtacgtgggt
     4021 atcggcaagc aggacggcgc ctggcctaag gacgtggccg ccgtcgtcaa gcgcctgacc
     4081 gggctacctg accgcttggt cgacgatatc gaggtgctcg agcgcttctc gttcgtcagc
     4141 gtgcccttcg aggccgcgga gaaggcgata gccgaggcga ggaagcagcg cggcgcgccg
     4201 cccgtgcgga tggccacgcc caagagcggc ccgggcggcc cgggcggagc gtccgggggc
     4261 aagcggggct acggcccgag gccgtcgtac ggctcgaagg gcggctacga ccgacccaag
     4321 ccgtacaaga agcctcgccc gcgcgactag cccggctcag gccagcgaca gggccttgtc
     4381 catcgtcgcc tggagcgtcc gcctgatgaa ggcgcgcagc ttggcgcgct tgtcgtcgtt
     4441 gacgctcgcg ggttcgaaca tgatcaccga tatcacgcct attcccggcg cgtcgtggct
     4501 gccgatcacg acgccgttca ggatcacgcg cacgcccttg aggttcagct gcatgtccct
     4561 gagcctggag ccctgcctcg gcgtggcccc gccggcgaag gcgaccgaca tgccgacgga
     4621 actcaggtcc ttgatccagc cgcgcagcag gccctcgtcg gtggggcagc tcagctcggt
     4681 cgagtccggg gggcaggccg cgcgcacgta gcgcctgcgg ccgcgcgcct cgttggcctc
     4741 gagcatgcgc aggagtatct cggtggtctt cgccacgccg attttcacga cgacgaagcc
     4801 gcactcgacg ccgagatcca tcaggtagta cgagcgcttg tcctcgtcgt cgagcatcgt
     4861 gatgatgccg aaggcggccc gggccgaagc ctcctcgccc ttgatggact tgatccacgc
     4921 gcgccagacg ctctcgtcgt cgccctcgtc gatgttgacg aaggtcaggc actccggctc
     4981 cttgcgcagg aagcgcgcca gcttggcgtg gtcgcgggtc gagtagacct cgaactcggc
     5041 ctcggccagg gcgttggcca cgtcgccgat cacgcccggc gggttcagga agaagacctt
     5101 cttgccgaac agtccattcc gttccagccc ctgatcctcc ggcggctggc tcgtttcgct
     5161 ttccatgcgt agaagagtat cgccgcggcg cggcgagcgc aagggcgggc gccctagtcg
     5221 tcgtccccgt cgatccggcg atacgaggcg gtctccctgg ccatcgccac gtactcgagg
     5281 ccgttccggc gtatgcgctc gagctcctcg tcgctgatcg tccgtacggc cttggccggg
     5341 ctgcccatga tcatcgagcg gggcgggaag gccttgccct gggtgacgag ggcgcccgcg
     5401 gcgacgatgg agccgggccc tatgcgcgag ccgttgagga gggtcgcgcc catgccgacg
     5461 aggcagtcgt cgccgacctc gcagccgtgc aggatgactc cgtgcccgac cgtcacgcgg
     5521 tcgccgacga cgcagggcgt ctcgtccgcc acgtgcacga ccgagccgtc ctggatgttc
     5581 gtgcccgagc cgatgcggat cggcgccatg tcggccctga ccgtcacgcc gaaccaaacg
     5641 gacgagtcct cgcctatggt cgcgtcgccg gccacctcgg cgttccacgc cacgaaggcg
     5701 ctcctcgcga cgcgcggtat ggcgtcgcct atggcatgca gcatagtccc tccgatcagg
     5761 ccgacagtat cgccgccggg ccccggcttg tcaaaatacc gccggcgccg atctttttcc
     5821 tttgcatcga cggacgatag tagataaggt atactagaga gacaaggaga agtgatggcc
     5881 cccgagcttg acgatgtcca cgaaataatc cagatcgatt ccgaattgaa taagatccgt
     5941 gattacgaca tcctgctcga gcgcgtcttg ctcgaggcgc gtcgcgtcgt ccacgccgac
     6001 gccggctcca tctacgtcaa ggaaggcgac gtcatctcga tcaagttcgc ccataacgac
     6061 accaagcaga agagcctccc gccgggcgag aagcaggcct atccccactt cagcatcccc
     6121 ataaacgata agaccatatc gggctacgtg gccatgaccg gcgaggccgt caacatcccc
     6181 gacgtctaca acctgccccc ggggctcccg ttcagctaca ataccgccta cgacgccaag
//
LOCUS       DAOPHV010000079         6223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_16435_length_6223_cov_3.435798, whole genome shotgun sequence.
ACCESSION   DAOPHV010000079 DAOPHV010000000
VERSION     DAOPHV010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16435_length_6223_cov_3.435798"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /gene="rpoN"
                     /locus_tag="PLQ29_03325"
     CDS             <1..1032
                     /gene="rpoN"
                     /locus_tag="PLQ29_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPG85708.1"
                     /translation="DPPVRANASCGAPASRRAGPRWACQTKPAFSSASRAASAITVAT
                     VDKVMAVIRRLEPSGACTRDFRESLLVQAEQAPGAPHGALEIIGDHMDLLERGRHQDI
                     QKRLKLDDDELEAALGFIKGLNPFPGRLYSAEETKYVTPDVQVKLKDGEFVIVINDEE
                     IPVIGINPFFDRLSGEKHDKETDAFVKDNIRKARWFIQSIQQRNRTLLKVVRSIVEFQ
                     RPFFARGPKHLAPLTLKDIAVEVGVHETTISRIAGKKYVQTDWGIFELRYFFTNSISG
                     SGSGGSRFSKGGVKQVIKEIIENETNAMSDQDITDILARKGIKLARRTVAKYRNELNM
                     DSSFGRKRG"
     gene            1071..1361
                     /gene="raiA"
                     /locus_tag="PLQ29_03330"
     CDS             1071..1361
                     /gene="raiA"
                     /locus_tag="PLQ29_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPG85709.1"
                     /translation="MTIDVRSVHFDLGEPSRAYLDKKLERIAYAKDLIVDLLFSFTKE
                     KQFKCECTVNFRWGSSAHVQETDFDLAAGMDKLIDTLEQKINKEKSKVQDKK"
     gene            1510..2499
                     /gene="hprK"
                     /locus_tag="PLQ29_03335"
     CDS             1510..2499
                     /gene="hprK"
                     /locus_tag="PLQ29_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HPG85710.1"
                     /translation="MDEKRFTVLDLLDLDLKEHDALELRCIGGRKGLGRVISAPDINR
                     PGLALSGFFESFAYQRVQLIGHGEAAYLHLLAAEGREEGVSRIFSFPIPCFIFANGEP
                     PAPMFAQAAEAANCPILLSDLPSTELSSRLMRVLTNVFAPRTSVHGVLVEVYGIGILL
                     TGESGVGKSETALELIERGHRLVADDVVEIRCISGNILMGSGANKVIGHHMEIRGLGI
                     INVTHLFGVGAIRDKKQIQLVCSLEEWDASKVYDRIGTEEKTMDILGVHVPQLDIPVK
                     PGRNIPIIVETAAMNERLKKMGYHSAKEFNQNILRWLESENARAVYFGRDDSI"
     gene            2525..2791
                     /locus_tag="PLQ29_03340"
     CDS             2525..2791
                     /locus_tag="PLQ29_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPG85711.1"
                     /translation="MIERMTVIKNRAGIHARPAALIVQTASKFKSKISLQKGDDRINA
                     KSIMGIITLGAGFDTEILIIADGEDEAEAAEAIDRLFKNRFEEV"
     gene            2791..4563
                     /locus_tag="PLQ29_03345"
     CDS             2791..4563
                     /locus_tag="PLQ29_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG85712.1"
                     /translation="MTGKGFTSRRAIDLVVIGLIYAIALSGSLAYLRTATLGDASFPA
                     VQSGAAAALLTLTPTVIGLVLVAAAARFAREYRRKAWGYRLKGRVLASFMAVAAMAAV
                     PPSLFIGILAVEALDAPASATVRSALGDGLDVALTYLADKEAALKYAAENDVAPIVDA
                     RGPDAERVLSRLSAREPSICAVELFSGGASASFAGDPTARGSPGFRPMDKSALLPRLS
                     AGGASYARYFAADAGSSPDRESRVSAIVSLRFDDEAELAASALSSTLATLEVSGRIRG
                     SSSLYIAFFSFSLVAPLLAIATLFALSVSDRLLRPVAALSDAITRIGSGARRAPYLAK
                     PGDEAGDLVESLNALLERIERSRGDELRNEKIGAWRDIARKLAHELRNPLTPIRLSAE
                     RVLKRWKADPSSVGAVLEKSMVAIIQETANMEALLTEFRDFARLPEPQKAWMELKPMV
                     DEAVHLYSASWPELTIDCGAIDPTITLKADRGYLKQALGNLLSNAAAATDGSGHVWIR
                     ADLVKTAESRYCRLQVRDDGYGIPADVRDKVFSPYFTTKPGGTGLGLAIVEHIVSAHG
                     GKIRFDSAEGAGTVFYIDLPAESQ"
     gene            4587..5951
                     /locus_tag="PLQ29_03350"
     CDS             4587..5951
                     /locus_tag="PLQ29_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPG85713.1"
                     /translation="MGAILVIDDEPGIRATVKDILEDEGYEALTAEDGPSGLEALKRD
                     SVDVVVLDVWLPRMGGIDVLKAIKADHPAVETIVVSGHASIDMAVSAVKLGAFDFIEK
                     PLSIERLITAVRNARALSDLRVENARLKRSAVESDDIIGASDPMAYVRDLIYQSARSD
                     ARVLITGENGTGKELVARRIHELSERASGPFVAVNCAAMPDTLIESELFGHEKGSFTD
                     AVARRKGKFETAHTGTLFLDEIADMSLSAQAKMLRAVQEMRFERLGGESTIEVDVRVI
                     AATNKDLRQEIAAGRFREDLFFRLNVIPLRIPALRERADDIPELTAFFLARFSGGRVR
                     RMSDEAMATLKAHDWPGNVRELKNFIERIAVMSDEDVISGETVRHFMLKSDGSSPEAA
                     PDRLDELLNIRYSEAKEAFERGYLHHNLRKHGYNIVKTAEAIGIYPKNLYAKIKKFGI
                     EAGE"
     gene            5948..>6223
                     /locus_tag="PLQ29_03355"
     CDS             5948..>6223
                     /locus_tag="PLQ29_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HPG85714.1"
                     /translation="MNDLTDRQKEVLDYIESFIWENPYPPTIREIAAHFSMSVRGAYD
                     HLKALERKGRIRAGGGRSRKIEIIGKSTRQEHNAAVEVPLLGSVAAGK"
BASE COUNT         1246 a         1994 c         1966 g         1017 t
ORIGIN      
        1 gatccgccag ttcgcgcgaa cgcatcttgc ggcgcaccag catcacgtcg agccggacca
       61 cgatgggcat gtcagacgaa gcccgcgttc tcgtcggcca gccgcgcggc ctcggccatc
      121 accgtggcca cggtggacaa ggtgatggcc gtcatccgca ggctggagcc ctccggcgcc
      181 tgcaccaggg attttaggga atcgctcctc gtccaggctg agcaagcccc cggcgcgccg
      241 cacggggcgc tcgagatcat cggcgaccac atggacctgc tcgagcgggg caggcatcag
      301 gacatccaga aacgcctcaa gctcgacgac gacgagctcg aggcggccct gggcttcatc
      361 aaggggctga accccttccc cggcaggctc tactcggccg aggagaccaa gtatgtcacc
      421 ccggacgttc aggtcaagct gaaggacggc gagttcgtca tcgtcatcaa cgacgaggag
      481 atacccgtca tcggcatcaa cccgttcttc gaccgtctat ccggcgagaa gcacgacaag
      541 gagacggacg ccttcgtcaa ggacaatatc cgcaaggcgc gctggttcat ccagagcatc
      601 cagcagcgga accgtaccct gcttaaggtc gtacgctcca tcgtcgagtt ccagcgcccg
      661 ttcttcgcga ggggacccaa gcacctcgcc ccgttgacgc tcaaggacat cgccgtcgag
      721 gtcggggtcc atgagacgac catctcccgc atcgccggga agaaatacgt ccagaccgac
      781 tggggcatct tcgagctgcg ctacttcttc acgaacagca tatccggctc cggctccggc
      841 ggctcgcgct tctccaaggg cggcgtgaag caggtgatca aggagatcat cgagaacgag
      901 acgaacgcga tgtccgacca ggacatcacc gacatactgg cccggaaggg catcaagctg
      961 gcgaggagga cagtggccaa gtaccgaaac gagttgaaca tggactcatc gttcggccgc
     1021 aagcgcggct aggcgcaatc agccggtctc accggcacaa ggaggcttgc atgactattg
     1081 acgtccgttc cgtgcacttc gacctcggcg aaccgagcag ggcttacctg gacaagaagc
     1141 tggagcgcat cgcgtacgcg aaggatctga tcgtcgacct cttgttctcg ttcaccaagg
     1201 agaagcagtt caagtgcgag tgcaccgtca acttccgctg gggctcgagc gcccacgtgc
     1261 aggagacgga cttcgacctg gccgcgggga tggacaagct gattgatacc ctcgaacaga
     1321 aaatcaacaa ggaaaagtcg aaggtccagg ataagaaata agctccacga gccgctcgat
     1381 cgaaggcccc gccgctcgcg ccgcggggcc ttcgccgtct cgggcgacgg ctccggggca
     1441 ggctatcgcg tcgagccgcc tcagttaata tcgttgcgca agcccgggaa acggagtatg
     1501 ctccgggcga tggatgagaa acgctttacg gtactcgact tgctcgacct cgacctcaag
     1561 gagcacgacg cgctcgagtt gcgctgcatc gggggccgca agggcctggg cagggtcatc
     1621 agcgccccgg acatcaaccg gccggggcta gccctgtccg gcttcttcga aagcttcgcc
     1681 taccagcgcg tccagctgat aggccacggc gaggcggcgt acctgcacct gctcgccgcc
     1741 gagggtcgcg aggaaggcgt ctcgaggatc ttctcgttcc cgataccgtg cttcatcttc
     1801 gccaacggcg aaccgccggc cccgatgttc gcccaggcgg ccgaggccgc caactgcccc
     1861 atcctgctat cggacctgcc ctcgacggag ctgtcgtcca ggctcatgcg cgtgctgacc
     1921 aacgtattcg cgccgaggac gagcgtgcac ggcgtgctcg tcgaggtgta cggcatcggc
     1981 atcctgctga ccggcgagtc gggcgtcggc aagagcgaga ccgcgctcga gctgatagag
     2041 cgcggccatc gcctcgtcgc cgacgacgtc gtcgagatac gctgcatatc cggcaacatc
     2101 ctgatgggtt cgggggccaa caaggtcatc ggccaccata tggagatccg cggcctcggc
     2161 atcatcaacg taacccacct cttcggcgtc ggcgccatca gggacaagaa gcagatccag
     2221 ctcgtctgct cgctggagga atgggacgca tccaaggtct acgaccgcat cgggaccgag
     2281 gaaaagacga tggacatcct gggcgtgcac gtgccgcagc tcgatatccc ggtcaagccg
     2341 ggcaggaaca tccccatcat cgtcgagacg gccgccatga acgagcggct caagaagatg
     2401 ggctaccatt cggccaagga gttcaaccag aatatactcc gctggcttga gagcgagaat
     2461 gcccgagccg tatactttgg gcgggacgat tcaatctagt ctatgtaaga tcggagaggc
     2521 ctccatgatc gagagaatga ccgtcatcaa gaacagggcg gggatccacg cccgacccgc
     2581 ggcgttgatc gtacagacgg ccagtaaatt caagtcgaag atatcgcttc agaagggcga
     2641 cgacaggata aacgccaaga gcatcatggg catcatcacg ctcggggcag gcttcgatac
     2701 cgagatcctc atcatcgccg acggcgagga cgaggcggag gccgccgagg ccatcgacag
     2761 gctgttcaag aaccgcttcg aagaggtgtg atgacgggca agggcttcac gtcgcgccgg
     2821 gcgatcgacc tggtcgtcat cggcctgatc tacgccatag cgctgtccgg ctcgctcgcg
     2881 tacctacgga ccgccacgct cggcgacgcg agcttccctg ccgtccagtc cggcgcggcg
     2941 gccgccctgt tgactctgac cccgacggtg ataggcctcg tgcttgtcgc ggcggccgcc
     3001 cgcttcgccc gggaatacag gagaaaggcc tggggctaca ggctcaaggg cagggtgctg
     3061 gcctcgttca tggccgtcgc cgcgatggcc gccgtgcctc cgtcgctatt catcggcatc
     3121 ctggccgtcg aggccttgga cgcgccggcc tcggcgaccg tccgctcggc gctcggcgac
     3181 gggctcgacg tggctctgac ctacctcgcg gacaaggaag ccgccctcaa gtacgccgcc
     3241 gagaacgacg tggcgccgat agtcgacgct cgaggcccgg acgccgagcg cgtgctgtcg
     3301 cggctgtcgg cccgtgagcc gtccatctgc gccgtcgagc tgttctcggg cggggcttcc
     3361 gcctccttcg ccggcgaccc gaccgcgcgc ggctcgcccg gcttcaggcc gatggacaaa
     3421 agcgcgctcc tacccaggct ctcggcgggc ggggcaagct acgcgcgcta tttcgcggcg
     3481 gacgccggaa gctcacctga cagggaatcg cgggtctcgg ccatagtctc gcttaggttc
     3541 gacgacgagg ccgagcttgc cgcgtcggcc ctatcgtcga ccctggccac gctcgaggtc
     3601 tccggccgca tacgcggctc ctcgagcctg tacatagcct tcttctcgtt cagcctggtc
     3661 gcaccgctcc tggccatagc cacgctgttc gcgctcagcg tatccgaccg gctgctcagg
     3721 ccggtcgcgg cgctctccga cgccataacc aggataggct cgggggccag gcgggccccg
     3781 tacctggcca agcccggcga cgaagccggc gacctggtcg agagcctcaa cgcgctgctc
     3841 gagcgcatcg aacgaagccg cggcgacgag ctacgcaatg agaagatagg ggcctggcgg
     3901 gatatcgcga ggaaactggc acacgagcta cgcaacccgt tgacgccgat ccgtctgtcg
     3961 gccgagcggg tactcaagcg ctggaaggcg gacccgtcgt cggtcggcgc ggtgctcgag
     4021 aagtccatgg tcgccatcat tcaagaaacg gccaatatgg aagcgttgct gaccgaattc
     4081 cgcgattttg cgagattgcc cgagccgcag aaggcctgga tggagctcaa gcccatggtc
     4141 gacgaggcgg tgcacctgta ctcggcctcg tggcccgagc tgaccataga ctgcggcgcc
     4201 atcgatccga ccattacgct gaaggcggat cggggttact tgaagcaagc gctcggcaac
     4261 ctgctgtcca atgccgccgc ggcgaccgat ggctcgggcc acgtgtggat acgggccgat
     4321 cttgtcaaaa cggcggaatc ccgatactgt agactccagg tcagggacga cgggtacggc
     4381 atcccggccg acgtccgaga caaggttttt tcaccgtact ttaccacgaa gcctggcggg
     4441 accggcttgg gcctcgccat cgtcgagcac atcgtttccg cccatggcgg caagatacgt
     4501 ttcgactccg cggaaggcgc gggtacggtt ttctacatcg acctgccggc ggagagtcaa
     4561 taatcgagca gtaaggaaga gtatccatgg gcgccatcct cgtaatcgac gacgagcccg
     4621 gcattcgggc caccgtaaag gacatcctcg aggacgaggg atacgaagcc ctgactgccg
     4681 aggacggccc gtccgggctc gaggcgctca agcgagacag cgtcgacgtc gtcgtcctgg
     4741 acgtatggct accgaggatg ggcggcatcg acgtactcaa ggcgatcaag gccgaccatc
     4801 cggcggtgga gaccatcgtc gtgtcgggcc atgcgtccat cgatatggcc gtcagcgccg
     4861 tcaagctcgg cgccttcgac ttcatcgaga agcccttatc gatcgagcgg ctgatcaccg
     4921 cggtcaggaa cgccagagcc ctgtcggatc tgcgcgtgga gaacgccagg ctgaagcgct
     4981 cggcggtcga gtcggacgac atcataggcg cctcggaccc gatggcctac gtacgcgacc
     5041 tgatctacca gagcgcccgc tcggacgccc gagtgctgat caccggcgag aacggcaccg
     5101 gcaaggaact cgtggccagg cgcatccacg agttgtcgga gcgggcctcg gggcccttcg
     5161 tagcggtgaa ctgcgccgcc atgcccgaca ccctgatcga gagcgagctg ttcggccacg
     5221 agaagggttc cttcaccgac gcggtagcca ggcgcaaggg caaattcgag acggctcata
     5281 ccggcacgct attcctcgac gagatagccg acatgtctct ctcggcccag gccaaaatgc
     5341 tccgggccgt ccaggagatg cgcttcgagc gcctcggagg cgagagcacc atcgaggtgg
     5401 acgtacgggt gatcgccgcc acgaacaagg atctcaggca ggagatagcg gccggccgct
     5461 tcagggagga cctgttcttc agactcaacg tcatcccgct acgtatcccc gccctgaggg
     5521 agcgggccga cgatataccc gagcttacgg ccttcttcct cgctcgcttt tcgggtggaa
     5581 gggtcaggcg catgtccgac gaggccatgg cgacgctcaa ggcccacgac tggccgggga
     5641 acgtgcgcga gctcaagaat tttatcgagc gtatagcggt gatgagcgac gaggacgtca
     5701 tctcgggcga gaccgtgcgg catttcatgc tcaagagcga cggctccagc ccggaagccg
     5761 ccccggaccg gctcgacgag ctattgaata ttcggtattc cgaggcaaaa gaagccttcg
     5821 aacgaggtta tttacaccac aacttgagaa aacatggcta taatatcgtg aagacagccg
     5881 aagcgatcgg gatttacccg aagaacctct acgccaagat caagaaattc ggtatcgagg
     5941 ccggcgaatg aatgacttga cggatcgcca aaaggaagtg ctcgattata tcgagtcctt
     6001 catctgggaa aacccctacc ccccgaccat acgcgagata gccgcccatt tttctatgtc
     6061 agtccgcggc gcgtacgacc acctgaaggc cctcgagcgc aagggccgca tcagagccgg
     6121 cggcggtcgg tccaggaaga tcgagatcat cggtaagtcg accaggcagg agcacaacgc
     6181 cgccgtcgag gtaccgttgc tcggctccgt ggccgcgggc aag
//
LOCUS       DAOPHV010000080         6187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_16557_length_6187_cov_4.005871, whole genome shotgun sequence.
ACCESSION   DAOPHV010000080 DAOPHV010000000
VERSION     DAOPHV010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16557_length_6187_cov_4.005871"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLQ29_03360"
     CDS             <1..470
                     /locus_tag="PLQ29_03360"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HPG85715.1"
                     /translation="CGLAGDILLLDKGRFFKQGVVAFLGGHVLYIAAFALSGLRPTWR
                     MLMPIAGYFGLYAIVLFNILLAKHAKYRPLAIAYLGGVLAMTVSALFADAANAARGAP
                     SYFFEGAFLFTVSDGVLSYRVFQRRFRFVDVAVLSTYYAAQCVIAANALFLAS"
     gene            complement(493..891)
                     /locus_tag="PLQ29_03365"
     CDS             complement(493..891)
                     /locus_tag="PLQ29_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85716.1"
                     /translation="MYHPKTIAWDSQLKAMFDRIDHELEDAYGGRWTLRRNRPERGAT
                     SNPEADGLFNVGTFFTPGYGSELGRGYLVEVILATDEAVPAAQRNEIEERVRDLLLDY
                     LPEYFPDRRLSVDRDGSMYKIHGETSLGRL"
     gene            944..1642
                     /locus_tag="PLQ29_03370"
     CDS             944..1642
                     /locus_tag="PLQ29_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85717.1"
                     /translation="MIILPGLTSTKKERIPAFLADMRRRGVKSIALFPTCLTRSERQA
                     LYAELEDVPGLGIPHVHLRSDCGAEEIGYLARRFGTEAFNIHPRASTHPFGPLPPEYA
                     PAIYVENVDIPPEEAELEGEGGPVPGGLCPDFSHLENARLMGMGEYVSAMDRLFGRFP
                     VGCCHLSAVRVGVPNQWSGQWDHHEFARLSDLDYLAVYRKRLPARWASLELENGLEEQ
                     LEAIDYLGGLLADR"
     gene            complement(1646..2908)
                     /locus_tag="PLQ29_03375"
     CDS             complement(1646..2908)
                     /locus_tag="PLQ29_03375"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPG85718.1"
                     /translation="MSQQMLLIAMTLEFAVFALMLGLLASQLRGKVRGPLEWACHALL
                     SFAASATYILLGGRYSIPLLFLANALLYAGILAIPIGLAYYLGERPRWGWCVFGWALG
                     LAIFFWFMVLQPNTAARVWAYSLFGASVFMFSAGYILKRAPDDMAVPTRIAAAFYAIY
                     SLLNLGRIVVFILRSRQVPVFGRLVGDEVFFVGALFIYPGMVFAELQLVSARLVLDLA
                     KAVDEKALMAREMNHRIKNSLALAESLVELQRGEGDDTAFGEALASIRSRLHSISLVH
                     ARLYHEGADGSIRADEYLGALAADLCYNLGYTDLRTNLEPLSVDAAVAVPLGVITNEL
                     ITNAIKYGNAPGSRRLELSLSERGGAEAILRVADGGPGFSENDKPGLGTTLVDSLAAQ
                     LDGSVRRFTEGGAVVEVTIPLPGRRSSR"
     gene            complement(2913..5102)
                     /locus_tag="PLQ29_03380"
     CDS             complement(2913..5102)
                     /locus_tag="PLQ29_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPG85719.1"
                     /translation="MTSYDRKAMRAIAANRLTGSRLALLAIAFYLCALPCAFAEDTAA
                     PDGRSQPRRVVEAGGDWAYAPFEYIDEAGYPAGFNVDVMRAIGDAVGIGFDIRLSSWA
                     EARERIERGDIDLLMGMYKSPERAEAVDFSSPHFYNVYGLFARKGSSVESLDDLVGHR
                     VAVQDGDRGHDYAVESGLGSNLVALRDWRDVFGAILRGEADYAIASALQGSIAVKQPE
                     FRSIEMVGPPLFSAEYCVAVRKGDEELLALIDEGLARIRSSGEYDTIYMRWFGHDLGP
                     GGNPRLGIVAAVATAALTVIAAALIWALSLRRLLTERTAELSAEQRRSREAGSKLQAA
                     LAEADEAARKAESAAEGRSAFIAWISQELRTPLQGILGAIDLLGRTRLDASQSKSLAM
                     ARSSSEQLNAVLTNILDAMGAERRTLRIEPAEFRYLEFASWLESELRPRAEDQGLGFR
                     FSARGQDRLVNADRRRISQVIMNLCANAIGYTDRGEVELSMALGDEALYVSVKDTGPG
                     LTEEARKHLFQPFYDASRIRKGAGIGLGLAQVKAIVDAMDGSIRYETSPSIGTRFEVS
                     LPIQSVADDGATEGEAGRESVTAEAPALTSGGRVIVAEDEAINRLYLKRLLETSGYTV
                     SPAGNGLDALEAAGSGSWDFILMDVSMPRMDGLEATRRIRAMEADRGLTRTPIIALTA
                     HAYAEDRQACVAAGMDGFLSKPFAEPALWDEVRRVSATVALNRPGQG"
     gene            5192..>6187
                     /locus_tag="PLQ29_03385"
     CDS             5192..>6187
                     /locus_tag="PLQ29_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85720.1"
                     /translation="MTIRTKILLPVIGVSALLFSVAGIVSVLTTVRTAIAGAEEQVVS
                     TSERYSFSLEATLEGPFATVDTLSTLFEGYALMPEHTRRETFAAMLRSIADRNADYVS
                     VWTAWGPGTIDRLDGELAGGSMANESGAFCIEYSKRAGSLSLDSLPDSIRSEKRFMSA
                     YGSLASSIVGPYQPKDGTGTVFSIVAPIIYGGKSLGVVGVEIESFDIVRMVNDLSLQT
                     GREYSLLDNDYVYLAVGDPDLLGRSILSVDPGRVDESEAVRGGLPLRMKFRSDDGESL
                     RVYTPVRISDTGKSWSLAVEVPTSEVRARSGAGSQMFMLFASFGAVLLAQLVVTII"
BASE COUNT         1024 a         2083 c         1991 g         1089 t
ORIGIN      
        1 catgcggcct ggcgggggat atcctgctgc tggacaaggg gcgcttcttc aagcagggcg
       61 tcgtcgcctt cctgggcggc cacgtcttgt atatcgccgc cttcgcgctc tccgggctga
      121 ggcctacctg gcgcatgctc atgcctatcg ccgggtactt cggcctctac gcgattgtgc
      181 tgttcaacat cctgctcgcc aagcacgcca agtatcggcc gctggccatc gcctacctcg
      241 gcggcgtgct cgcgatgacg gtgtcggccc tcttcgccga cgccgctaac gccgcccgag
      301 gcgcgcccag ctatttcttc gagggggcct tcctgttcac cgtgtcggac ggcgtgctgt
      361 cgtaccgcgt cttccagcgg cgcttcaggt tcgtcgacgt ggcggtgctg tccacctact
      421 acgctgccca gtgcgtcatc gcggcgaacg cgctgttcct ggcgtcgtga cgcccccggg
      481 ggccgaggcc ggctagagcc taccgaggct ggtctcgccg tggatcttgt acatcgagcc
      541 gtcccggtcg acgctcagcc tgcggtccgg gaagtactcg ggcaggtaat cgagcaacag
      601 gtcgcgtacc cgctcctcga tctcgttccg ctgggccgcg ggcaccgcct cgtcggtggc
      661 taggatcacc tcgacgagat agccacgccc gagctcgctg ccgtaacccg gcgtgaagaa
      721 cgtgcccaca ttgaagagcc cgtccgcctc ggggttcgag gtggcgccgc gctccgggcg
      781 gttgcgccgg agcgtccacc tgccaccgta ggcgtcctcc agctcatggt cgatgcggtc
      841 gaacatggcc ttgagctgac tatcccaggc gatcgtctta gggtggtaca taggcgtcct
      901 ccggcgcttg acccgccgcg ccggggcgga acatcatggc gccatgatca tactaccagg
      961 tcttaccagc acgaagaaag agcgtatccc cgccttccta gccgatatgc gccgccgcgg
     1021 agtcaagagc atagccctgt tcccgacctg cctgacccgc tcagagcgcc aagccctgta
     1081 cgccgagctc gaggacgtcc ccggcctcgg gatcccccac gtccacctgc gctccgactg
     1141 cggcgctgag gagatcggct atttggcgag gcgcttcgga accgaggcct tcaatatcca
     1201 tccgcgcgcg agcacgcatc cgttcgggcc gctgccgccc gagtacgcgc ccgcgatcta
     1261 cgtcgagaac gtcgatatac cgcccgagga ggcagagctg gaaggcgagg gcggtccggt
     1321 tcccggcggg ctgtgcccgg acttctcgca cctcgagaac gcgaggctga tgggcatggg
     1381 cgagtacgtt tcggctatgg accggctatt cgggcgtttc ccggtcggtt gttgccacct
     1441 gtcggccgtc agggtaggcg tgcccaacca gtggtccggc caatgggacc atcatgagtt
     1501 cgcgcgactg tccgacctgg actacctggc cgtctaccgg aaacgcctgc ccgcgcgctg
     1561 ggcgtccctg gagctggaga acggcctcga ggaacagttg gaggccatcg actacctggg
     1621 cggattgctg gcggatcgat agggcttagc gactcgacct gcggcccggt agcgggatcg
     1681 tcacctcgac gaccgcgccg ccctcggtga agcgcctgac cgaaccatcc aattgggcgg
     1741 ccagcgagtc gaccagcgtc gtgccgaggc cgggcttgtc gttctcggaa aatccggggc
     1801 cgccgtccgc tacacggagg atcgcttcgg ccccgcctcg ctcggacagc gacagctcca
     1861 atcgcctcga gcccggcgcg ttgccgtact tgatggcgtt cgtgatcagt tcgttcgtga
     1921 tgacgcccag cggcacggcc acggccgcgt cgaccgagag cggctcgagg ttcgtcctca
     1981 ggtcggtgta gccgaggttg tagcataggt cggccgcgag cgcacctaag tactcgtcgg
     2041 cgcgtatcga gccgtccgcg ccctcgtggt atagcctggc gtgaacgagg gaaatcgagt
     2101 gcagcctcga gcggatcgaa gcgagcgcct cgccgaaggc cgtgtcgtcg ccctcgcccc
     2161 gctgcagctc gacgaggctc tcggccaggg ccaggctgtt cttgatccgg tggttcatct
     2221 cccgcgccat tagggccttc tcgtccacgg ccttggccaa gtccagcacc agcctggcgc
     2281 tgacgagctg tagctcggcg aagaccatgc cgggatagat gaacagcgcg ccgacgaaga
     2341 agacttcgtc gccgacgagc cggccgaata ccggtacctg tcgcgagcgt aggatgaaga
     2401 cgactatgcg gcccaggttc aggagcgagt agatcgcata gaacgccgcc gctatcctcg
     2461 tcggaacagc catgtcatcg ggagcccgtt tcagtatata gccggcgctg aacatgaaga
     2521 cgctcgcccc gaatagggaa taggcccaga cgcgcgcggc cgtattgggc tggagaacca
     2581 tgaaccagaa aaaaatcgcc aagcccaggg cccagccgaa gacgcaccag ccccatcgcg
     2641 gacgctcgcc gaggtagtac gctagtccga tggggatagc tagtatgcct gcgtagagta
     2701 gggcgttggc caggaatagc agcggaatcg aataccggcc gccaagcagg atgtaggtgg
     2761 ccgatgcggc gaacgacagg agcgcgtggc aggcccattc gagcggcccg cgcaccttgc
     2821 ccctgagctg ggacgccaac aggccgagca tgagcgcgaa cacggcgaat tcgagcgtca
     2881 tggctatcag gagcatctgc tgggacatgc ggctatcctt gtcccgggcg attgagcgcc
     2941 acggtcgccg agaccctgcg cacctcgtcc cagagcgctg gttcggcaaa gggcttggac
     3001 aggaagccgt ccatgccggc ggctacgcag gcctgcctgt cctcggcgta ggcgtgggcc
     3061 gtcagcgcga tgatgggcgt ccgggtaagg cctcggtccg cctccatggc gcggatgcgg
     3121 cgggtagcct cgaggccgtc catcctgggc atgctcacgt ccatcaggat gaagtcccac
     3181 gaacccgacc cggcggcctc gagcgcatcc agtccgttgc cggcaggcga taccgtgtaa
     3241 ccggacgtct cgagcagccg cttcaggtag agcctattga tcgcctcatc ctcggcgacg
     3301 ataacccggc ccccgctcgt gagcgcgggc gcctcggcgg tcactgattc tctccccgcc
     3361 tcgccttcgg tagccccgtc gtcggcgacc gactgtatag gaaggctcac ctcgaagcgg
     3421 gtgccgatgc tcgggctggt ctcgtagcgt atcgagccgt ccatggcatc gacgatggcc
     3481 ttgacctggg ccagcccaag gccgataccg gcgcccttcc gtatcctgct cgcgtcgtag
     3541 aacggctgga acaggtgctt cctggcctcc tcggtcaggc ctgggccggt atccttgacc
     3601 gagacgtata gggcctcgtc gccgagcgcc atcgataact caacctcacc ccggtcggta
     3661 tagccgatgg cgttggcgca gaggttcatg atcacctgcg atatgcgtcg ccggtcggcg
     3721 ttgacgagcc ggtcttggcc acgggccgag aacctgaacc cgaggccctg gtcctccgcc
     3781 ctcgggcgaa gctcggactc gagccatgaa gcgaactcca ggtacctgaa ctccgcgggc
     3841 tctatccgta gcgttctccg ctcggcgccc atcgcgtcga ggatattcgt tagcaccgcg
     3901 ttgagctgct ccgaggacga acgggccata gccaacgatt tcgactggct cgcgtccagc
     3961 cgggtcctac ccaggaggtc gatggcgccg agtattccct gcaagggcgt tcgtagctcc
     4021 tggctgatcc acgcgatgaa ggcggaccgg ccttccgccg ccgactccgc cttgcgcgcc
     4081 gcttcgtcgg cctcggcgag cgccgcctgc agcttcgagc ccgcctcgcg cgagcgcctc
     4141 tgctcggcgg atagctcggc cgtccgttcg gttaggagcc ggcgcagcga cagggcccag
     4201 atcaacgcgg ccgcgattac ggtcagggcc gcggtcgcca cggcggcaac gatcccgagc
     4261 cgcgggtttc ctccggggcc caggtcgtgg ccgaaccatc gcatgtagat cgtgtcgtac
     4321 tcgccgctcg agcgtatccg cgcgagaccc tcgtcgatca gcgccagcag ctcttcgtcg
     4381 cccttcctga ccgccacgca gtactcggcg ctgaatagcg gggggccgac catctcgatc
     4441 gacctgaatt ccggctgctt gaccgcgatg gagccctgca gggccgacgc aatggcatag
     4501 tccgcctctc cccgcaggat ggcgccgaac acgtcgcgcc agtcgcgtag cgcgacgagg
     4561 ttcgagccga gcccgctctc gaccgcgtag tcatggccac ggtcgccgtc ttggacggcg
     4621 actcgatggc cgaccagatc gtcgagcgac tcgacgctcg agcccttgcg cgcgaatagt
     4681 ccgtagacat tatagaaatg cggcgacgag aaatcgaccg cctcggcgcg ctcagggctc
     4741 ttgtacatgc ccatgagcag gtcgatgtcg cctcgctcga tccgctcccg ggcctcggcc
     4801 caggacgata gccgtatatc gaagccgata cccaccgcgt cgcctatcgc cctcatgacg
     4861 tcgacattga agcccgccgg atagcccgcc tcgtcgatgt actcgaaggg cgcataggcc
     4921 cagtctccgc cggcctccac gacgcggcgc ggctggctcc ggccgtccgg ggctgccgta
     4981 tcctcggcga aggcgcatgg caaggcgcac agatagaacg ctatggccag gagcgcgagg
     5041 cggcttcccg ttagccggtt tgcggcgatc gcgcgcatgg cttttctatc gtacgatgtc
     5101 atcgccatga atgatacgtc attccaactc gccgcgcaaa ctattcctac tattgggcaa
     5161 aaacgggtat actgagaggt aggaggcagc cttgaccata cgtacgaaga tactgctacc
     5221 ggtgatcggc gtatccgccc tgctgttcag cgtggcgggg atagtctcgg tgctgactac
     5281 ggtccgtacg gccatcgccg gagcggagga acaggtggtc tccacctcgg agcggtactc
     5341 cttcagcctg gaggcgaccc tcgaggggcc gttcgccacc gtcgacacgc tcagcaccct
     5401 gttcgaaggc tacgcgctga tgccggagca tacgaggcga gaaaccttcg ccgctatgct
     5461 ccgctccatc gccgatagga acgccgacta cgtctccgtc tggaccgcct gggggccggg
     5521 tacgatagac cgtctcgacg gcgagctggc cggcggctcc atggccaatg agagcggtgc
     5581 tttctgtata gagtactcga aacgggccgg aagcctgagt ctcgactcgc tacccgactc
     5641 gatacgctcc gagaagcgct tcatgagcgc gtatggctcg ctcgcctcgt ccatcgtcgg
     5701 cccatatcag cccaaggacg gcaccggcac ggtcttcagc atcgtcgcgc ccatcatcta
     5761 cggcggcaag tcgctcggcg tcgtcggcgt ggagatagag tcgttcgaca tcgtcaggat
     5821 ggtcaacgac ctgagcctgc agaccggcag ggagtactcg ctgctcgaca atgactacgt
     5881 gtacctggcc gtaggcgacc cggatctgct cggccggtcc atcctgagcg tcgatccggg
     5941 ccgcgtcgac gaatcggaag ccgtgcgcgg aggcctgccg ctgcgcatga agttccggtc
     6001 cgacgacggc gagagcctgc gggtctatac cccggtccgc atctccgata ccggcaagtc
     6061 ctggtcactc gcggtcgagg tgccgacgag cgaggttcgc gccaggtccg gggccggctc
     6121 ccagatgttc atgctgttcg cgtcgttcgg cgccgtgctg ctcgcccaac tcgtcgtgac
     6181 gatcatc
//
LOCUS       DAOPHV010000081         6163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_16631_length_6163_cov_5.725933, whole genome shotgun sequence.
ACCESSION   DAOPHV010000081 DAOPHV010000000
VERSION     DAOPHV010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16631_length_6163_cov_5.725933"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLQ29_03390"
     CDS             <1..193
                     /locus_tag="PLQ29_03390"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HPG85721.1"
                     /translation="VWVGALAISMALTMLSLFLSSTFKRVLGVQLLSAIERLMGLVLT
                     AVSIDMIMSGAAAFIEAAI"
     gene            223..504
                     /locus_tag="PLQ29_03395"
     CDS             223..504
                     /locus_tag="PLQ29_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85722.1"
                     /translation="MSVFEIIMLLCFGAAWPVSIAKSLRTRSNGGKSLVFLLIIIVGY
                     AAGIVNKLLYKPDFVVWLYALNTAMVSVDAALWIRNRRLERAATPEARA"
     gene            600..1412
                     /locus_tag="PLQ29_03400"
     CDS             600..1412
                     /locus_tag="PLQ29_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HPG85723.1"
                     /translation="MGLGSCATVPAEPEPSAAIVLSVVGTPVVTAVDLDTVSVSAELL
                     AANTGGRAAALSALRAEATSDGVALAAPAVVELGGPGARAELAPGAERSVPVELVLDA
                     PPGDGPMVSFALLSVLAFSSPSGPGEARLELRGEFPRIMPPAMSISSIRILKDELINT
                     KLGVELVVSNPNAFALTFAELDYRLYGEGRYWASGCLAKPFVVPAGEATKASLYLTMN
                     FTDMSRSLLDQVIKLATVSYRLVGSGRVDTGLEFLPRFELPFDMSGRAEVGR"
     gene            complement(1409..>2192)
                     /locus_tag="PLQ29_03405"
     CDS             complement(1409..>2192)
                     /locus_tag="PLQ29_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase PurH"
                     /protein_id="HPG85724.1"
                     /translation="HLSPCGVAESGVAESGVATTDGESPARALEAAVACDPLSAFGGV
                     VAVNRSFDADSVRALGETFVECVAAPSFTPEARGLLAKRKNARLLEMGDAPSRTAVGY
                     ETRSIRGGFLRQEIDDGDPDGTEWKVVSKRAPTEAELAALRFAWKACVSAKSNAILLA
                     APIDAADPSAGYATVGIGSGQPNRVDSTRIAVGRAGDKARGSVLASDAFFPFPDSIEV
                     AAAAGVSAIAHPGGSIRDGLSVEAADAAGIAMIVTGVRHFRH"
     assembly_gap    2193..2292
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2293..3147)
                     /locus_tag="PLQ29_03410"
     CDS             complement(<2293..3147)
                     /locus_tag="PLQ29_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase PurH"
                     /protein_id="HPG85725.1"
                     /translation="MPIALISVSDKTGLVAFAGRLEKAGWRLLASGGTAKAIAAAGVA
                     VDEVADYTGSPEILGGRVKTLHPAIHGGILSRGTDADRSELAGFGWSELDLVVCNLYP
                     FEATMARPGAAEAACVEEIDIGGVALLRAAAKNYARVTVVCDPADYERVAASLETSVG
                     ESGGLGPELRRELAVKAFALCARYDAAIASWLEPESSVGPFGYAGQKLRYGENPHQKA
                     WLYTDEPGAGPLGGSVLQGKELSYNNLLDLDAAWRTAAGFDGAAAVVVKHLSPCGVAE
                     SGVAESGVA"
     gene            complement(3223..3891)
                     /gene="purN"
                     /locus_tag="PLQ29_03415"
     CDS             complement(3223..3891)
                     /gene="purN"
                     /locus_tag="PLQ29_03415"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPG85726.1"
                     /translation="MSGPGRRLRVVVLASGNGTNLQALLDASAAGALRAEVAAVVSDR
                     TGAYALERARLAGLTAVEAPFERGGDRRAYDAALAEAVATHDPDYVLLLGWMRILSSA
                     FLTRFPGRVINLHPALPGTFPGVRAIERAFEAYRTGAIDRTGVMTHLVPDEGVDSGPV
                     LAVEEVPILDGDDLGSLEARVHAVERRLIVKTVKGLVRKNNHGGTETRRKTEESGGEG
                     YGIP"
     gene            complement(3888..>6163)
                     /gene="purD"
                     /locus_tag="PLQ29_03420"
     CDS             complement(3888..>6163)
                     /gene="purD"
                     /locus_tag="PLQ29_03420"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPG85727.1"
                     /translation="RSPSCGGLVIAPGNPGTAALGRNVPISMTDVVGLVALAEAEAVD
                     LVVVGPEVPLALGLADELAGRGIACFGPSRAAAALESSKAFAKATMASLGVPTADYRV
                     CTDFDEAAAFVRAAPWPLVVKASGLASGKGVVLPDSADEAVGALHAMMRGGRLGDAAR
                     EVVVEERLEGEELSLMAFCDGSRLALMPGARDHKRLLDGDRGPNTGGMGAYAPACTTE
                     LAAEYARLTMEPLVDSMRARGTPFVGVLYAGLIVTPSGPKVLEYNCRFGDPEAQAVLA
                     LFAGDLAATMAACAAGDLRKAPPSWKTGAAACVVMAGDGYPERPVGGAVLSGLEPLAD
                     GRASVARVLHANTRLEGGRLVSGGGRVLSVVAAGDKLPEAVAAAYAKADEVSFPGARY
                     RRDIGARELARLAADKPRRSSAYASSGVDIDAGNEAVRRMSAAVKATYSEAVLAGIGS
                     FGGLYDATSIKTMAQPVLVASTDGVGTKVKLAAQAGSYASIGQDIVNHCIDDILVQGA
                     RPLMFLDYFASSRLDPAMVAEAVGGIAAACRAAGCALIGGETAEMPGVYRDGEFDVAG
                     TILGVVERGSILPAVGVGPGDALVGFASSGPHTNGYSLARKAFESVDLSTVYPELGRP
                     LGEALLAPHRSYLPLLWPVLSGSPGLVKALAHITGGGFIENLPRVLPDGVDAVVRRGS
                     WPVPPLYGRIERLGRVEPDEMYRVFNMGVGMVAVVDSGRVDELRALVGEECWVIGELV
                     GSAQGGKARARLDGERA"
BASE COUNT          891 a         2141 c         2133 g          898 t
ORIGIN      
        1 cgtctgggta ggcgcgctgg ccatctcgat ggcgctgacc atgctcagcc tgttcctctc
       61 ctcgaccttc aagcgcgtcc tcggggtgca gctcctgtcg gcgatcgagc gcctgatggg
      121 actcgtcctg acggcggtgt cgatagacat gatcatgagc ggcgccgcgg ccttcatcga
      181 ggcggcgatc tagggcgggc cgtggcccgt cgaaaggaaa ccatgagcgt attcgagatc
      241 atcatgctgc tatgcttcgg cgccgcctgg cccgtctcga tagcgaagtc gctacggacg
      301 aggagcaacg gaggcaagag cctcgtgttc ctcttgatca tcatcgttgg ctacgcggcc
      361 ggcatcgtca acaagctgct ctacaagccc gacttcgtcg tatggctcta cgcgctcaat
      421 acggcgatgg tgtcggtgga cgccgcgctg tggatacgca accgccggct cgagcgggcg
      481 gctaccccgg aggccagggc gtgagggctt gcggcgtttc gcgattcggg cgccgcgggc
      541 ggcgggccgc gaggcgcctc cggtccggtg cggcctcggc cctcgtattc tgcgccgcgt
      601 tgggcctcgg ctcgtgcgcg acggtcccgg ccgagcccga gccgagcgcc gcgatcgtcc
      661 tgtcggtcgt cgggacgccg gtcgtgacgg ccgtcgacct cgataccgtc tcggtcagcg
      721 ccgagctact cgcggccaat accggcggcc gggcggccgc cctgtccgcg ctgcgcgccg
      781 aggcgacgtc ggacggcgtc gccctggccg cgccagcggt cgtcgagctc ggcggcccgg
      841 gggctcgcgc cgagctggcg ccgggcgccg aacggtccgt acccgtcgag ctcgtcttgg
      901 acgcgccgcc gggggacggg ccgatggtat cgttcgccct gctgtcggtc ctggccttct
      961 cgtcgccctc ggggccgggc gaggccaggc tcgagctccg cggtgagttc ccgaggatca
     1021 tgccgcccgc gatgagcatc tcgtcgatac gcatcctcaa ggacgagctg atcaacacga
     1081 agctcggcgt cgagctggtg gtgagcaacc cgaacgcgtt cgcgctgacc ttcgcggagc
     1141 tggactaccg gctgtacggc gaggggcgct actgggcgtc cggctgcctg gccaagccct
     1201 tcgtcgtccc ggccggcgag gcgacgaagg ccagcctcta cctgacgatg aacttcaccg
     1261 acatgagccg atccctgctc gaccaggtga tcaagctggc gaccgtcagc taccgcctgg
     1321 tcggttccgg ccgcgtcgac accgggctcg agttcctgcc gcgcttcgag ctgcccttcg
     1381 acatgtccgg ccgggccgag gtcggccgct agtgcctgaa gtgcctgacc ccggtgacga
     1441 tcatcgcgat gcccgcggcg tcggccgcct cgacgcttaa cccatcgcgg atggagccgc
     1501 ccgggtgcgc gatcgcgctt acgccggccg ccgccgccac ctctatcgag tcggggaacg
     1561 ggaagaacgc gtccgaggcc agcaccgagc cgcgcgcctt gtccccggcc cgccctacgg
     1621 cgatgcgggt cgagtcgacg cggttcggct ggccggagcc tatgccgact gtcgcgtagc
     1681 cggcgctcgg gtcggccgcg tcgataggcg cggccagcag gatggcgttg gacttggccg
     1741 acacgcaggc cttccatgcg aagcgtagcg ccgcgagctc ggcctcggtc ggagctcgct
     1801 tggagacgac cttccactcg gttccgtcgg gatccccgtc gtcgatctcc tgcctgagga
     1861 aaccgccgcg tatagagcgc gtctcgtatc cgaccgcggt tcgcgagggg gcgtctccca
     1921 tttcgagcag gcgggcgttc ttgcgcttgg cgagtagccc gcgagcctcg ggcgtgaacg
     1981 acggggcggc gacgcactcg acgaaggtct ccccgagcgc gcggactgag tcggcgtcga
     2041 aggaccggtt gaccgccacg acgccgccga aagcggacag cgggtcgcag gcgacggccg
     2101 cttcgagggc gcgagccggc gactcgccgt ccgtcgtggc gacgccgctt tcggcgacgc
     2161 cgctttcggc gacgccgcag ggcgataggt gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnggcgacgc cgctttcggc gacgccgctt tcggcgacgc cgcagggcga
     2341 taggtgcttg acgacgaccg cggccgcccc gtcgaaaccg gcggccgtcc gccaggccgc
     2401 gtccaggtcg agcaggttgt tgtacgagag ctccttgccc tggagcacgc tgccgcccag
     2461 gggcccggcg cccggctcgt cagtgtagag ccaggccttt tggtgagggt tctcgccgta
     2521 gcgcagcttc tggccagcgt aaccgaacgg ccccacgctg gattccggct cgagccacga
     2581 ggcaatggcc gcgtcgtagc gggcgcatag cgcgaaggcc ttgaccgcca gctcgcgcct
     2641 gagctccggc ccgaggcctc cagactcgcc gacgctcgtt tcaagcgagg ccgcgacgcg
     2701 ctcgtagtcc gcgggatcgc agacgacggt gacgcgcgcg tagttcttgg cggcggcgcg
     2761 caggagggcg acgccgccta tgtcgatctc ctcgacgcag gccgcctcgg ctgcgcctgg
     2821 ccgcgccatc gtcgcctcga aggggtagag gttgcatacg accaggtcaa gctccgacca
     2881 gccgaagccg gccagctccg agcggtcggc gtccgtgccg cgcgacagga tgccgccgtg
     2941 gatcgcgggg tgcagcgtct tgactcggcc gcctagtatc tccggcgagc cggtgtagtc
     3001 cgcgacctcg tcgacggcca cgcccgccgc ggcgatggcc ttggccgtac cgccggaggc
     3061 gaggaggcgc cagccagcct tttccagccg ccccgcgaac gcaacgaggc ccgtcttgtc
     3121 ggataccgat atcaacgcta taggcataca cgctcctcga aacaaaaatt aggaatttga
     3181 accaaagaaa cacggaggca cagagaaaaa agaatcaata agtcatggta ttccatatcc
     3241 ttctcctccg ctttcctccg tcttcctccg cgtctccgtg cctccgtggt tgtttttgcg
     3301 taccaagcct tttacggttt ttactataag gcggcgctcg acggcgtgta cccgcgcctc
     3361 gagcgatccg aggtcgtcac cgtccaggat gggcacctcc tcgacggcca ggaccggccc
     3421 ggagtcgacg ccctcgtcgg gtacgaggtg ggtcatgacg ccggtgcggt cgatggcgcc
     3481 ggtccggtag gcctcgaagg cccgctcgat cgcgcgcaca ccggggaagg tgccgggcag
     3541 ggcaggatgc aggttgatga cgcggcccgg gaagcgcgtc aggaaggccg acgataggat
     3601 gcgcatccag ccgagtagca gcacgtaatc agggtcatgc gtcgctacgg cttccgcgag
     3661 cgcggcgtcg taggcccgcc ggtcgccgcc gcgctcgaac ggcgcctcga cggcggtaag
     3721 tcccgccagt ctcgcgcgct cgagcgcgta ggcgccagtc ctgtcggaaa cgacggcagc
     3781 tacctcggcc cgcaaggctc cggcagccga ggcgtcgagc agggcttgca gattcgtgcc
     3841 gttcccggac gcgaggacca cgaccctcag gcgacgtccg gggccgctca cgctcgctcc
     3901 ccatcgagtc gcgcgcgagc cttcccgccc tgcgccgagc cgaccagctc gccgatcacc
     3961 cagcactcct ccccgacgag cgcgcgcagt tcgtcgaccc ggccgctgtc gacgacggcc
     4021 accatgccga cgcccatgtt gaacacgcgg tacatctcgt cgggctcgac gcggccaagg
     4081 cgctcgatac ggccgtacag cggcggtacc ggccaggagc cgcgcctgac cacagcgtcg
     4141 acgccgtccg gcaggacgcg aggcaagttc tcgatgaagc cgccgcccgt gatatgagcg
     4201 agcgccttga cgaggcccgg cgagccggac aggaccggcc acaggagcgg caggtacgag
     4261 cgatgcggcg cgagcagggc ctcgccgagc ggccgaccga gctccgggta gacggtcgac
     4321 aggtcgacgc tctcgaaggc cttgcgcgcc agcgagtagc cgttcgtgtg ggggccggat
     4381 gaagcgaacc cgacgagggc gtcgccgggt ccgacgccga cggccggcag tatggagccg
     4441 cgctcgacga cgccgagtat cgtgccggcc acgtcgaact cgccgtcccg gtagacgccg
     4501 ggcatctccg cggtttcgcc gcctatgagc gcgcaaccgg cggcccgaca agcggccgca
     4561 atgccgccga cggcttcggc gaccatcgcg gggtcgagcc tggagctggc gaagtagtcg
     4621 aggaacatga gcgggcgggc gccctgcacg agtatgtcgt cgatgcagtg gttaacgatg
     4681 tcctggccta tggaggcgta ggagccggcc tgggcggcca gcttgacctt ggtgccgacg
     4741 ccgtccgtcg aggcgaccag tacgggctgc gccatggtct ttatgctcgt agcgtcgtag
     4801 agcccgccga aggagccgat gccggcgagt accgcctccg agtaggtggc cttgaccgcg
     4861 gccgacatgc gcctgactgc ctcgttgccg gcgtcgatgt cgacgccgga gctagcgtac
     4921 gctgaactcc gacgaggctt gtccgcggcg agcctggcga gttcccgggc gccgatgtcg
     4981 cggcgatagc gagcgccggg gaatgatacc tcgtcggcct tggcgtaggc cgcggctacg
     5041 gcctcgggca gcttgtcgcc ggccgcgacg acggatagga cgcgcccgcc gccggagacg
     5101 aggcgcccgc cctccaacct cgtattggcg tgcaggaccc gggcgaccga agcgcgtccg
     5161 tccgccaacg gctcgaggcc ggacagtacg gctccgccga ccggccgctc ggggtacccg
     5221 tcgccggcca tcacgacgca ggcggccgct cccgttttcc aggacggtgg cgccttccgc
     5281 aggtcgccgg ccgcgcaagc cgccatggtg gcggccaaat cgccggcgaa cagcgccagc
     5341 acggcctggg cctccgggtc gccgaagcgg caattgtatt cgagcacctt cgggcccgag
     5401 ggggtgacga tgagccctgc gtacaggacg ccgacgaacg gcgtcccccg cgcgcgcatc
     5461 gagtcgacca gcggctccat cgtcagcctg gcgtactcgg cggccagctc cgtcgtacag
     5521 gctggcgcgt aggcgcccat gccgccggta ttcggccctc ggtccccatc caggagccgc
     5581 ttatggtccc gcgcgccggg catgagcgcc aggcgcgacc cgtcgcagaa ggccatcagg
     5641 ctgagctcct cgccctcgag tcgctcctcg accacgacct cccgggcggc gtcgccaagg
     5701 cgcccgccgc gcatcatcgc gtgcagggcg ccgaccgcct cgtcggccga gtccggcagg
     5761 accacgccct tgccggaggc caggccggag gccttgacga ccagcggcca gggcgcggcc
     5821 cggacgaagg cggccgcctc gtcgaagtcg gtacaaaccc tgtagtcggc cgtcggcacg
     5881 ccgaggctgg ccatcgtcgc cttggcgaag gccttggacg actcgagcgc cgccgccgcc
     5941 cggctcgggc cgaagcaggc tatgccgcga ccggccagct cgtccgcgag gccgagggcc
     6001 agcgggacct cgggcccgac gacgaccagg tcgaccgcct cggcctcggc gagcgcgacg
     6061 aggccgacca catcggtcat gctgatgggc acattacggc cgagcgccgc ggtgcctgga
     6121 ttacccggcg ctatgacgag cccgccgcac gacggcgagc ggc
//
LOCUS       DAOPHV010000082         6140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_16739_length_6140_cov_3.833854, whole genome shotgun sequence.
ACCESSION   DAOPHV010000082 DAOPHV010000000
VERSION     DAOPHV010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16739_length_6140_cov_3.833854"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PLQ29_03425"
     CDS             complement(<1..519)
                     /locus_tag="PLQ29_03425"
                     /inference="COORDINATES: protein motif:HMM:NF016349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HPG85728.1"
                     /translation="MDKLRRELAPISQEAWNEIDAMARETLKASLSGRKIVDVHGPRG
                     FDYAGVPLGRLSVPKQQPSGEVRYGLHQVLPLVETRVDFSLDIWELDNIGRGARDPDL
                     DALVEACRKIAAFEEKAVFDGFKAGSFIGLHEYAKDRKVGAVLNSDAFLDAVAEGQAM
                     MRRDGVDAPANLV"
     gene            complement(537..890)
                     /locus_tag="PLQ29_03430"
     CDS             complement(537..890)
                     /locus_tag="PLQ29_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPG85729.1"
                     /translation="MPDFGNPFSGLKHERKLTHEELIRAVRFMVSAEYEAIQLYQQLA
                     ESTDNGLAQKVLYDIADEEKVHAGEFLRLLKELDPEEEGFYKEGAQEVEEEFLGGSSA
                     SPTSGQGLGIGGIKN"
     gene            complement(1007..2863)
                     /gene="aspS"
                     /locus_tag="PLQ29_03435"
     CDS             complement(1007..2863)
                     /gene="aspS"
                     /locus_tag="PLQ29_03435"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPG85730.1"
                     /translation="METLKRTVTCGALTGADAGKTVTLNGWVNRKRDHGGISFINLRD
                     RYGTTQVVVDADAPESLKAIANELKSEYCVAVEGTVRARPDSMVNPDMATGQIEVVAS
                     NLVVLTKCDVLPFQIDDETDAKEELRLRYRYLDLRSGGMQRRIKLRNDVAFAVREHMI
                     GQGFYEIETPTFIKSTPEGARDYLVPSRLYPGKFYALPQSPQLYKQILMVSGFDKYFQ
                     IARCYRDEDARGDRQPEFTQIDIEMSFVGRDDVLAMVEGLMGHVFRKTMGLELPAAFK
                     RLSYDEALELYGTDKPDLRFGMEMRDFEPYVAASTFQAFKDALATGAAEREANAAKGV
                     TIQGGGVKALVVPGKADYSRKQIEELEAAARIYRAKGMAWMKVVAASGDSSGEGGKPS
                     LEGGASKFFSANASEIIAGLGAKPGDLILLVADAKRRVANVALGAVRTKLGRDLGLCD
                     PKRFEFAWIVDFPMFEWNEDEQKWDTAHHMFTWPQEKYHPTLESDPGSVKGDLYDLVL
                     NGFELASGSIRIHDPALQKRIFKIVGFDEAEGEKKFGFLTEAFKYGAPPHGGIAPGLD
                     RLVMLMAGETSIKEVIAFPKNSFAVSPMDDCPSEVDQKQLDELHLSIRYPEK"
     gene            complement(2977..4236)
                     /locus_tag="PLQ29_03440"
     CDS             complement(2977..4236)
                     /locus_tag="PLQ29_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85731.1"
                     /translation="MLNKRGAVLAFALSAIAIGAWAQAKPTVGVLSVDSPLYSSAVVS
                     ESKVAARLVAGDPLLIVGSQDKKIAIDGVPTLWYQVKTAEGTAGWIPGSRLAFSPTAF
                     KRSAFHSEEQYSAYFLMAVRPGDKVIAARSYEKVAKGDVGYYVSYHDGGLPIAIVWER
                     NLAATPAEEYLPSDFPEALQQFVYYVTFPVVELAGESGVASFSQLAKDLPKRYPSADG
                     FYASEIDEEFPWYTPPEASYGYSDGYDDDYGYYDDYGDEDYSDYDEDYVEGVESYGMI
                     KVGSTVILGKHDDVNGGANWADEMDAYVGTEAVVSSLPGADSQGFLVVRVNGNGYVWR
                     VRNLTLQGRGEAGSYGYQVGDKVIIGAHRYIDEDNNWASDMLEYVGRTATITTMEGTD
                     GSECYIVHVDIDNGDWYWRVETMQPAK"
     gene            complement(4282..5361)
                     /locus_tag="PLQ29_03445"
     CDS             complement(4282..5361)
                     /locus_tag="PLQ29_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HPG85732.1"
                     /translation="MDFNRFSEGVRELVEKTQLFGSEPLEAVLLANPRAGGFSRPARA
                     AALTRDLAQAVSDASALPARSASLSWRVAQTSGPRHATRLASEYLEEAAMKPGRAWLI
                     VLACGDGTSLEFLDELSRAPDELRSRFTVLRLPMGTGNDGSDGRELSDSLSRLTGRGA
                     IAVQPTLRVIPAPGGPASRRAPGGEWRAFNIASVGLDAYVTDTTNKLKTSLPGDSYKL
                     VLDLASVLYDKLYPPREMIITARSSDGTELSRREGRFLLVAMGVSGRRTYGSNKPILP
                     DDDNVCAVDQMPLLRKVALKGPVSSGKIRDFPEASLFSADLVEIAYADRILVQMDGEA
                     ELLVAEDFPLRFEKTAPLIRHIARA"
     gene            5482..5886
                     /locus_tag="PLQ29_03450"
     CDS             5482..5886
                     /locus_tag="PLQ29_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85733.1"
                     /translation="MDFSWKIIIDAGIISAALIVATFLRTKLRFLQRFMVPNSLTAGV
                     LLLPVYNYLMPALGYTQNRLGDLVYHLLNISFIAMTLRSKPKGPAKDSSHSVWAMSTA
                     IISQYSLQALFGLLLTWVLFSTVLPGLNPAFG"
     gene            5907..>6140
                     /locus_tag="PLQ29_03455"
     CDS             5907..>6140
                     /locus_tag="PLQ29_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HPG85734.1"
                     /translation="MGPGQAYAIGKGWEGMGFEGAGSEGLTMAALGYLWACVVGVALV
                     NLGVRCGYLPKNAQTAMADRAMLTGIMPREREKP"
BASE COUNT          974 a         1924 c         2031 g         1211 t
ORIGIN      
        1 gaccaggttg gccggggcgt cgacgccgtc ccggcgcatc atcgcctggc cttcggctac
       61 tgcgtccagg aaggcgtcgg aattgaggac ggcgcctacc ttcctgtctt tggcgtactc
      121 gtgcagcccg atgaaggagc cggccttgaa gccgtcgaag accgccttct cctcgaaggc
      181 cgcgatcttg cggcaggctt cgaccagcgc gtccaggtcc gggtcgcgcg ctccgcggcc
      241 gatattgtcc agctcccaga tatcgaggct gaagtccacc ctggtctcca cgaggggcag
      301 gacctggtgc aggccgtagc gtacctcgcc ggagggttgc tgtttgggta cggatagtct
      361 gccgagcggt actccggcgt agtcgaagcc gcgcggtcca tggacgtcga caatcttacg
      421 ccccgagagg ctggccttga gggtttccct ggccatggcg tcgatctcgt tccacgcttc
      481 ctggctaatg ggggccagtt cccgtcgtag tttgtccatc cgacgctcct ttcgtttcag
      541 tttttgatcc cgccgatgcc gagcccctgg cccgaggtcg gagaagcgct cgatcctccg
      601 aggaattcct cctcgacctc ctgggcgcct tctttataga agccttcctc ttcaggatca
      661 agctccttga gcaggcgcag gaactcgccg gcgtggacct tctcctcgtc ggctatatcg
      721 tagagcacct tttgggccaa gccgttgtcg gtcgattcgg ccagctgctg gtagagctgg
      781 atagcctcgt attcggctga gaccatgaag cgtacggctc ggatgagctc ctcgtgggtg
      841 agcttgcgct catgcttgag accgctgaaa ggattaccga aatccggcat gggtcacctc
      901 catataatgt atacatagaa tagagttgtg aaacgccgtt cgtcaaatga aaagcccggc
      961 gcacggccgg gcttcatact aaatgtcggt ctatcggcca gtccggctac ttctcaggat
     1021 accggatcga caggtgcagc tcgtcgagct gcttctggtc gacctcgctc ggacagtcgt
     1081 ccatcgggga gaccgcgaag gagttcttgg ggaaggcgat gacttccttg atgctcgtct
     1141 cgccggccat cagcatgacg aggcggtcga gcccgggcgc tatgccaccg tggggcgggg
     1201 cgccgtactt gaaggcctcg gtcaggaagc cgaacttctt ctcgccctcg gcctcgtcga
     1261 agccgacgat cttgaagatg cgcttctgga gggccgggtc gtgaatgcgg atggagcctg
     1321 aggccagctc gaagccgttc aggacgaggt cgtagaggtc gcccttgacc gagccgggat
     1381 cggactcgag ggtagggtgg tatttctcct gcggccaggt gaacatgtgg tgggccgtgt
     1441 cccatttctg ctcgtcctcg ttccactcga acatggggaa gtcgacgatc caggcgaact
     1501 cgaagcgctt cgggtcgcag agtcccaggt cgcggccgag cttggtccgt accgcgccga
     1561 gggccacgtt ggccacgcgg cgcttggcgt ccgcgacgag gagtatcagg tcgcccggct
     1621 tagcgccgag gccggctatg atctccgagg cgttggctga gaagaacttg gatgctccgc
     1681 cctcgaggct gggcttgccg ccttcgccgg acgagtcgcc gctagcggcg acaaccttca
     1741 tccaggccat acccttggcg cggtatatcc tggccgcggc ctcgagttcc tcgatctgct
     1801 tgcgcgagta gtcggccttg ccggggacga cgagcgcctt gacgccgccg ccctggatcg
     1861 tgacgccctt ggccgcgttg gcctcccgct cggcggcgcc ggtggcgagc gcgtccttga
     1921 aggcctggaa ggtggacgcg gcgacgtagg gctcgaagtc gcgcatctcc atcccgaagc
     1981 ggaggtccgg cttgtcggtg ccgtacagct cgagcgcctc gtcgtaggac aggcgcttga
     2041 acgcggcggg cagctcgagg cccatcgtct tcctgaacac gtggcccatc aggccctcga
     2101 ccatggccag cacgtcgtcg cggccgacga acgacatctc gatgtctatc tgcgtgaact
     2161 cgggctgcct gtcgccgcgc gcgtcctcgt cgcggtagca gcgggctatc tggaagtact
     2221 tatcgaatcc ggagaccatc aggatctgct tgtatagctg ggggctctgc ggtagcgcgt
     2281 agaacttgcc cgggtacagg cggctcggca cgaggtagtc tcgggctccc tccggcgtgc
     2341 tcttgatgaa ggtcggggtc tcgatctcgt agaagccctg gcctatcatg tgctcgcgga
     2401 cggcgaacgc cacgtcgttg cgcagcttga tgcggcgctg catgccgccg gagcgtaggt
     2461 cgaggtagcg gtagcggagc ctgagctcct ccttcgcgtc cgtctcgtcg tcgatctgga
     2521 agggtaggac gtcgcacttg gtcagtacga cgaggttcga cgcgacgacc tcgatctggc
     2581 cggtagccat gtctgggttg accatactgt cgggcctggc gcgcaccgtc ccctcgaccg
     2641 cgacgcaata ctcgctcttg agctcgttcg ctatcgcctt gagggattcg ggcgcgtcgg
     2701 cgtccaccac cacttgggtg gtgccgtagc ggtcgcggag gttgatgaag ctgattccgc
     2761 cgtggtcccg cttgcgattg acccacccgt tcagggttac cgtcttgcct gcatcggccc
     2821 cggtcagggc gccgcaggtc acggtgcgct tgagtgtttc catagtaacg cgactatatc
     2881 gcggcgccgg gccggtcgtc caggggaggg acgataggcg ccctatatgg aaggagcccg
     2941 cctcgcggcg ggctcctcgg aatcgggatg gcggccttac ttggccggct gcatcgtctc
     3001 gacgcgccag taccagtcgc cgttgtcgat gtcgacgtgg acgatatagc attccgagcc
     3061 gtcggtgcct tccatcgtcg tgatggtggc cgtgcggccg acgtactcga gcatatcgct
     3121 cgcccagttg ttgtcctcgt cgatgtagcg gtgggcgccg attatgacct tgtcgcctac
     3181 ctggtagccg tagctgccgg cctcgcccct gccttggagc gtcaggttgc ggacgcgcca
     3241 gacgtagccg ttgccgttga ccctgacgac caggaagcct tggctgtcgg ctcccggcag
     3301 ggacgagacg acggcctcgg tgccgacgta ggcgtccatc tcgtcggccc agttggcgcc
     3361 gccattgacg tcgtcgtgct tgccgaggat caccgtgctg ccgaccttga tcataccgta
     3421 gctctcgacg ccctcgacgt agtcctcgtc gtagtcgctg tagtcttcgt cgccgtagtc
     3481 gtcgtagtag ccgtaatcgt catcgtagcc gtcggaatag ccgtaggagg cctcgggcgg
     3541 cgtataccag gggaattcct cgtcgatctc ggaggcgtag aagccgtcgg cggaggggta
     3601 gcgcttgggt aggtccttgg ccaactggct gaaggaggct acgcctgact cgcctgcgag
     3661 ctcgacgacg gggaacgtga cgtagtagac gaattgctgc agagcctcgg ggaagtccga
     3721 tggcaggtat tcctcggccg gagtggccgc gaggttgcgt tcccagacga ttgcgatcgg
     3781 caggccgccg tcgtggtagg agacgtagta gccgacgtcg cccttggcca ccttctcgta
     3841 gctgcgagcc gcgatgacct tgtcgccggg cctgacggcc atcaggaagt aggcggagta
     3901 ctgctcctcg ctatggaagg ccgagcgctt gaaggcggtc ggcgagaacg ccagcctgga
     3961 gcccggtatc cagcccgcgg tgccctcggc ggtcttgacc tggtaccaga gcgtcggcac
     4021 gccgtcgatc gctatcttct tgtcctggga gccgacgatg agcaggggat ctccggcgac
     4081 gagcctcgcg gccaccttgg attcgctcac cacggccgac gagtagagcg ggctgtcgac
     4141 ggacagtacg ccgacggtcg gcttggcctg ggcccaggcg ccgatcgcta tcgccgagag
     4201 agcgaacgcc aggaccgcac ctcttttatt aagcatacga ttcctttcta tgcgctcgcg
     4261 gtcggcgaat cgccttcgcc gctatgctcg cgctatgtga cggatgagcg gcgcggtctt
     4321 ctcgaagcgc aacggaaaat cctccgcgac caggagctcc gcctctccgt ccatctgtac
     4381 tagtatacga tcggcgtacg cgatttcaac gaggtccgct gaaaaaagcg aggcctcggg
     4441 gaagtcccgt atcttgcccg atgacacggg acccttgagg gcgaccttgc gtaggagtgg
     4501 catctggtca accgcgcaga cgttgtcgtc gtcgggcaga atgggcttat tcgacccgta
     4561 ggtgcgccgt ccggacacgc ccatggccac caggaggaag cgcccctcgc gcctggacag
     4621 ttcggtcccg tcggacgagc gggccgttat gatcatctcc ctaggcggat acagcttgtc
     4681 gtagagaacc gacgccaggt cgaggacgag cttatacgag tcgccgggta ggctcgtctt
     4741 gagtttgttg gtcgtatcgg tcacgtaggc gtcgaggccg acgctggcga tattgaaggc
     4801 tctccattcg ccgcccggag cgcgccgcga cgcggggccg cccggggcag gaatcacgcg
     4861 gagcgtcggc tgcacggcga tggcgccgcg tccggtaagc ctggagagcg agtcggacag
     4921 ctctcggccg tccgagccgt cgttgccggt acccatgggc agccggagga ccgtaaagcg
     4981 gcttcgtagc tcgtctggcg ccctggacag ctcgtcgagg aactcgaggc tggtgccgtc
     5041 gccgcaggcg agcacgatca gccaggccct gcccggcttc atcgcggctt cctcgaggta
     5101 ctcggaggcg agcctggtgg cgtgcctggg gcccgaggtc tgggctaccc gccacgacag
     5161 gctggcggac cgagccggca gggctgaagc gtcagagacc gcttgcgcga ggtcgcgcgt
     5221 cagcgcggcg gccctggcgg gcctcgagaa accgccggcc ctgggattag cgagcagtac
     5281 cgcctcgagc ggctccgatc cgaagagttg cgtcttttcg actagttcac gaacgccttc
     5341 cgagaagcgg ttaaaatcca tggcagggag tgtactacgg aagcccccgg ttgaaaagcg
     5401 ttctctatcg ccatacgtat ggtagactcg tcgtcgaggc gccgagggcc ggctctggcc
     5461 gttaggcggg aaaggactac tatggacttc tcatggaaga tcatcatcga cgccggcatc
     5521 atctccgcgg cgcttatcgt cgccaccttc ttgcggacga aactccgctt cttgcagcgc
     5581 ttcatggttc cgaattcgct gacggcgggc gttctcctgc tgcccgtcta caattacctg
     5641 atgccggccc tcggctatac ccagaaccgg ctcggcgacc tggtctacca cctactcaat
     5701 atatcgttca tcgccatgac gctacgctcg aagccgaagg ggccggccaa ggactcgtcc
     5761 cactcggtct gggccatgtc gacggccatc atctcgcagt actccttgca ggccctgttc
     5821 gggctcctgc tgacctgggt cctgttctcg accgtgttgc cgggcctgaa cccggccttt
     5881 ggctgacgct accgctcggc ttcgcgatgg gacccggcca agcctacgcg atcggcaagg
     5941 gctgggaagg catgggcttc gagggcgccg gttcggaggg cctgacgatg gccgccctcg
     6001 gttacctctg ggcctgcgtc gtcggcgtgg ccctcgtcaa cctcggcgtc aggtgcggct
     6061 acctgcctaa gaacgcccag acggccatgg ccgaccgcgc catgctgacc ggcatcatgc
     6121 ccagggagcg ggagaagccg
//
LOCUS       DAOPHV010000083         6120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_16822_length_6120_cov_3.550701, whole genome shotgun sequence.
ACCESSION   DAOPHV010000083 DAOPHV010000000
VERSION     DAOPHV010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16822_length_6120_cov_3.550701"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PLQ29_03460"
     CDS             <1..715
                     /locus_tag="PLQ29_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPG85735.1"
                     /translation="LTVSGVYHCGYFDYDAALVFLPESAAGELGRGEARVVGVKLRDR
                     YGDERAMAALAAAGLEGAESWRVYNRAFFGALRMEKSVMLLLVGLIFIVVGVNIFHSM
                     RKAVYGRVEDIATMKALGAGSSSVRRVFMLDGAAAGAGGAIIGLVVGLLVVTNVNGVF
                     GAIEAAVAAVYGALGGRGPGFEFFSPELFYIGDVPVRLSFPETLFIASAGALAAVAAA
                     WAASSRVSRIMPSEVLRDE"
     gene            708..1388
                     /locus_tag="PLQ29_03465"
     CDS             708..1388
                     /locus_tag="PLQ29_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG85736.1"
                     /translation="MSDDVIVCRGVRKVFASEAEDLEILRGVDLSLPSGAIASITGPS
                     GCGKSTFLSILGGLDRPSAGEASVAGVDLIRADEAGLSAFRATAVGFVFQFHYLLKDF
                     TALENAMLPAYMLTGNRAEAIGRARELLEAVGLGGRLDHVPAKLSGGERQRVAIARAL
                     VNAPGVVLADEPTGNLDRQSALAVEDLLFDLAARRGATLLVVTHDPGMAARADRRYIL
                     SDGTLVES"
     gene            1385..2686
                     /locus_tag="PLQ29_03470"
     CDS             1385..2686
                     /locus_tag="PLQ29_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPG85737.1"
                     /translation="MTGSPIAFVATRFFFGRTAADGSRDQARKALAGAVLSVALSLVP
                     LITVMQVADGMIQGISARYVELGTYHAQALPYASADLVGARDAARSTPGVAGAWIETQ
                     SVGVAFANGEREGAAVRGVEPGFLEDPSTLAYLRVEAGEPTLERPYDALVGQAMAEKL
                     GLGPGDVVNLISMRKAADGELLPRVTMLTVRGVVSAGYRDLDAQWLFMRQDTAARVLP
                     KENTRSFVGIKADDPFGDPFAARDAVEGRLPPGFTAYSWREVERNLFESLASTRTMLL
                     LIMAVTVAVAAVNVSSALQTLVLERGQEIAVMQSLGASPSDLARIFTLGGAAIGALGA
                     VLGAAGGLLASVRVNELLAFIEAVANSARSAAARLSGGAAATRFRVLDPAYYLETIPV
                     DVPYGEIAMVVAATVALSFVAALGPARRAASLSPLDGFRRR"
     gene            2770..3978
                     /gene="recA"
                     /locus_tag="PLQ29_03475"
     CDS             2770..3978
                     /gene="recA"
                     /locus_tag="PLQ29_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPG85738.1"
                     /translation="MAKNETIVANQTPDEKLKALEAARLQIEKQYGVGSLMQLGKYDN
                     PLGIEAIPSGSILLDEALGVGGYPRGRIIEIYGPESSGKTTLALHAIAEAQKKGGIAA
                     FIDAEHALDPVYAKNLGVNTDDLWVSQPDNGEQALEIAESLVRSGAVDIVVVDSVAAL
                     TPQAEIEGDMGDAQMGLQARLMSQALRKLTGTLSKSKTILIFINQIRMKIGVMFGNPE
                     TTTGGNALKFYSSIRVEVRRIETIDGSNDEAVGNKVRVKIVKNKVAPPFRRVELDIMF
                     GKGISSIASLLDGAVKTGVADKKGAWYTYGEEKLGQGRENAKATLETNQELADEIEAK
                     VRAIMFPGKSPEAAGGKKPAVGTKAPSPATVPAGTASAQPPRPQPAAKAVPTDGRAAR
                     PAVDEDGGLF"
     gene            3990..4496
                     /gene="folK"
                     /locus_tag="PLQ29_03480"
     CDS             3990..4496
                     /gene="folK"
                     /locus_tag="PLQ29_03480"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPG85739.1"
                     /translation="MGLGSNVGDSLATLRGALEALGAFLSGLRSSSVYRSAPRYVVDQ
                     ADFLNLVALGETDLAPAELLARTQAVEASFGRDRTKERRKGPRSLDVDVLLYGDLVID
                     LPELLVPHPGMTERAFVLVPLAELSPALVDPRDGQPFSHRLGDVADQGIYLHAGPPLY
                     CRNDAWKQ"
     gene            4484..5254
                     /locus_tag="PLQ29_03485"
     CDS             4484..5254
                     /locus_tag="PLQ29_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPG85740.1"
                     /translation="MEAINEASLEPERAGRSYRLAEFEGPLDLLLFLIKKNEVNIYDI
                     PIASITEQFIASLSEAEAADLDDLTEFYMLAATLLYIKSRMLLPVEMNLADEIEDPRR
                     DLVEKLIEYQKFKKLSELMEKKELEVEWTIERTKMQRPLPFGDEELWDKIDVWDLLKS
                     FSGLMGGMSSERIIDMYEEVSINEKTALIHELLESRESFGFHDLVTRSGSLLDIVCAF
                     LAVLEAVKYRVISIHQHRLFGDIQIRRHERRGTDGTEP"
     gene            5238..5789
                     /gene="scpB"
                     /locus_tag="PLQ29_03490"
     CDS             5238..5789
                     /gene="scpB"
                     /locus_tag="PLQ29_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPG85741.1"
                     /translation="MELNLDREAAILETILFLESEPVDKAALSRISGLGIDVTEAALD
                     HLRECLASACHGIELVEMGGGWLLVPKRDLWEGLKDRYGRKNEQKLSRAALETLSIIA
                     YSQPVTRAEVEAIRGVAADTMIRFLLDKGFIKEVGKKDAPGKPIQYGTTKDFLKFFRL
                     GSISDLPKLDELERDRFELEGRA"
     gene            5786..>6120
                     /locus_tag="PLQ29_03495"
     CDS             5786..>6120
                     /locus_tag="PLQ29_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HPG85742.1"
                     /translation="MSSGEDGTRIQALIARAGLASRRAAEECMRAGRVRLNGRVVTEQ
                     GRRVDPDSDAIRVDGARIPPPRRHAYVVFNKPRGVVSTMDDPEGRPTISDYLGKHSRE
                     RLYHVGRLDT"
BASE COUNT         1030 a         2057 c         2063 g          970 t
ORIGIN      
        1 cctgaccgtc tcgggcgtct atcactgcgg ctacttcgat tacgatgcgg cgctcgtctt
       61 cctgcccgag tcggcggcgg gggagcttgg ccgcggtgag gcccgcgtcg tcggcgtcaa
      121 gctgcgcgac cgctacggcg acgagcgcgc catggccgcc ctcgccgccg ccggcctcga
      181 gggcgccgaa tcgtggcgcg tctacaaccg cgcgttcttc ggcgcgctca ggatggagaa
      241 atcggtgatg ctgctactcg tcggcctcat cttcatcgtc gtcggcgtca atatcttcca
      301 ttcgatgcgc aaggcggtct acgggcgcgt cgaggacata gcgacgatga aggccctcgg
      361 cgccggctca tcgagcgtca ggcgcgtgtt catgctcgac ggcgcggccg cgggagccgg
      421 cggggccatc ataggactcg tcgtcgggct gctcgtggtg acgaacgtca acggcgtgtt
      481 cggcgcgatc gaggccgcgg tcgcggcggt ctacggcgcc ttgggcgggc gagggccggg
      541 cttcgaattc ttctcgcctg agctgttcta tataggagac gtaccggttc ggctcagctt
      601 tccggagacg ctgttcatag cgtcggctgg agcgctcgcc gctgtcgccg cggcctgggc
      661 ggccagttcc agggtttccc gcatcatgcc ttcggaggtg cttcgcgatg agtgacgacg
      721 tcatagtctg ccgcggcgtg cgcaaggtgt tcgccagcga ggccgaagac ttggagatac
      781 tgcgcggggt cgacctaagc ctgccgagcg gcgccatcgc ttcgatcacc ggtccgagcg
      841 gctgcggcaa gagtaccttc ctatcgatac tcggcggcct cgatcgcccg agcgctggag
      901 aggcatcggt cgccggcgtc gacctgatac gggccgacga agccgggctg tcggcgttcc
      961 gggctacggc cgtcgggttc gtcttccagt tccactacct gctcaaggac ttcaccgcgc
     1021 tcgagaacgc gatgctgccg gcctacatgt tgaccggtaa ccgcgccgag gcgatcggcc
     1081 gcgccaggga actgctcgag gccgtgggcc tcggcggacg gctcgaccac gtcccggcca
     1141 agctgtcggg cggcgagcgc cagagggtcg ccatcgcccg ggccctcgtc aacgctccgg
     1201 gagtcgtgct cgccgacgag ccgacgggca acctggacag gcagagcgct ctggccgtcg
     1261 aggacctgct gttcgacctg gcggccaggc gcggcgcgac cctattggtc gtcacccacg
     1321 acccgggcat ggcggccagg gccgaccggc ggtacatact gagcgacggg acgctggtcg
     1381 agtcatgacc ggctcgccga tagccttcgt ggcgaccagg ttcttcttcg ggcgcacggc
     1441 cgccgacgga agccgcgacc aggccaggaa ggccctggcc ggagccgtgc tatcggtcgc
     1501 gctgagcctc gtgccgctca tcacggtcat gcaggtcgcc gacggcatga ttcagggcat
     1561 cagcgcccgg tacgtcgagc tcggcaccta ccacgcccag gccctgccgt acgcctcggc
     1621 cgacctcgtc ggggccaggg acgcggcgcg ctcgactccg ggcgtcgcgg gcgcttggat
     1681 cgagacgcag tcggtcggcg tcgcgttcgc caacggcgag cgcgagggcg cggccgtgcg
     1741 cggcgtcgag ccgggcttcc tcgaggaccc gtcgaccctg gcttatctgc gcgtcgaggc
     1801 gggcgagccg acgctcgagc ggccgtacga cgccctcgtc ggccaggcga tggccgagaa
     1861 gttgggtctc ggaccgggcg acgtggtcaa cctgatatcg atgcgcaagg ccgcggacgg
     1921 cgagctcctg ccgcgcgtca cgatgctgac cgtgcgcggc gtggtgtcgg ccggctatcg
     1981 agatctggac gcccaatggc tgttcatgcg gcaggatacg gcggcccgcg tcctgcccaa
     2041 ggagaatacc cgtagcttcg tcgggatcaa ggccgacgac ccgttcggcg acccgttcgc
     2101 cgcccgagac gcggtcgagg ggcgcctgcc tcccggcttc accgcgtact cctggcgcga
     2161 ggtggagcgg aatctcttcg agtcgctcgc aagcacgagg acgatgctgc tcctgatcat
     2221 ggcggtcacc gtcgcggtcg cggccgtcaa cgtgtcgagc gcgctccaga ccctcgtgct
     2281 cgagcggggc caggagatag ccgtcatgca gagcctcggg gcgagcccgt ccgacctggc
     2341 caggatcttc accctgggcg gggcggcgat aggcgcgctc ggggcggtcc tgggcgcggc
     2401 gggcgggctc ctggcgagcg tccgagtcaa cgagctgctc gccttcatcg aggccgtggc
     2461 caactcagcc cgatcggcgg cggcccgcct gtcgggcggc gcggccgcga cgcgcttcag
     2521 agtgctcgac cccgcgtact acctcgagac gataccggtc gacgtaccct acggcgagat
     2581 agccatggtc gtcgccgcga cggtcgcgct gtccttcgtg gcggcgctcg ggccggcacg
     2641 cagggcagcc tcgctgtctc cgctcgacgg tttccgcagg cgatgatgac aattccgccc
     2701 cgccgcgcta tagtggccgg gcgaacgcgt tcgtgatcgt agccgcgtat gcagtaccag
     2761 gaggaccaca tggcaaagaa cgagactata gtcgcgaacc agacccccga cgagaagctc
     2821 aaggctctcg aggcggcgcg cctgcagata gagaagcagt acggcgtcgg ctcgctcatg
     2881 cagctcggca agtatgacaa cccgctcggc atcgaggcta tcccgtccgg ctccatcctg
     2941 ctcgacgagg cgctcggcgt cggcggctac ccgcgcgggc gtatcatcga gatctacggg
     3001 ccggagtcgt ccggcaagac cacgctggcg ctacacgcga tcgccgaggc ccagaagaag
     3061 ggcggcatcg cggccttcat cgacgcggag cacgcgctcg acccggtcta cgccaagaac
     3121 ctcggagtga ataccgacga cctgtgggtg tcgcagccgg acaatggcga gcaggctctc
     3181 gagatagcgg agtctctcgt ccgctccggc gcggtcgaca tcgtcgtcgt cgactcggtc
     3241 gcggccctga cgccccaggc ggagatcgag ggcgacatgg gcgacgccca gatgggcctc
     3301 caggcccgcc tgatgagcca ggccctgcgc aagctcaccg gcacgctctc gaagtccaag
     3361 accatcctga ttttcatcaa ccagatacgc atgaagatag gcgtcatgtt cggcaacccc
     3421 gagacgacga ccggcggcaa cgccctcaag ttctactctt cgatccgtgt cgaggtgcgg
     3481 cgcatcgaga cgatagacgg cagcaacgac gaggccgtcg gcaacaaggt gcgcgtcaag
     3541 attgttaaga acaaggtagc gccgccattc aggcgcgtcg agctcgatat catgttcggc
     3601 aagggcattt cgtcgatagc cagcctcctc gatggagcgg tcaagaccgg cgtcgccgac
     3661 aagaagggcg cctggtacac ctacggcgag gagaagctcg ggcaaggccg cgagaacgcc
     3721 aaggccacgc tcgagaccaa ccaggagctg gcggacgaga tcgaggccaa ggttcgcgcc
     3781 atcatgttcc ccggtaagtc gcccgaggcg gccggcggca agaaaccggc cgtgggaacc
     3841 aaggctccgt ctccggcgac cgttccggcc ggtaccgcgt cggcgcaacc gcccaggccg
     3901 cagccggccg ccaaggctgt gccgaccgac ggccgggctg ccagaccggc cgtggacgaa
     3961 gacggcgggc tcttctgagc ctcgtctacc tggggctcgg ctcgaacgta ggcgacagcc
     4021 tggccaccct gcgcggcgct ctcgaggccc tgggcgcttt cctgtcgggc ctgcgctcct
     4081 cgtcggtcta cagaagcgcg ccgcgctacg tcgtcgacca ggccgacttc ctcaacctcg
     4141 tcgccctcgg cgagactgat ttggcgccgg ccgagctcct ggccaggacg caggccgtcg
     4201 aggcgagctt cgggcgcgac cggaccaagg agcgccgcaa ggggccgcgc agcctcgacg
     4261 tcgacgtcct cctgtacggc gacctcgtca tcgatttgcc cgaactgctc gtgccgcatc
     4321 ctggcatgac ggagcgcgcc ttcgtgctcg tgccgctcgc ggagctgtcg ccggccctcg
     4381 tcgaccctcg ggacgggcag ccgttctccc accgcctcgg cgatgtggcc gaccagggga
     4441 tttacctgca cgccggcccg ccgctatact gccggaacga cgcatggaag caataaacga
     4501 agcatcgctc gagcccgagc gagctgggcg tagctatcgc ctggccgaat tcgagggacc
     4561 cctcgacctc ctgctcttcc tcatcaagaa gaacgaggtc aatatctacg atatacctat
     4621 agcctcgatc accgagcagt tcatcgcctc tctgtccgag gccgaggccg ccgacctgga
     4681 cgacctgacc gagttctata tgctcgcggc gaccctgctg tatatcaaga gccgtatgct
     4741 cctgccggtc gagatgaacc tggccgacga gatcgaggat ccgcggcgcg acctcgtcga
     4801 gaagctgatc gagtaccaga agttcaagaa gctatccgaa ctcatggaaa agaaagagct
     4861 cgaggtcgag tggaccatag agcggaccaa gatgcaacgg cccttgccct tcggcgacga
     4921 ggagctatgg gacaagatcg acgtctggga cctactcaag tcgttctccg gcctgatggg
     4981 cggcatgtcg agcgagcgca tcatcgacat gtacgaggag gtgtcgatca acgagaagac
     5041 ggcgctgatc catgagctgc tcgagtccag ggaatccttc ggcttccacg acctggtcac
     5101 gcgctcgggt tccctgctcg acatcgtctg cgccttcctg gccgtgctcg aggcggtcaa
     5161 gtaccgcgtc atatcgatac accagcatcg cctgttcggc gatatccaga tacgtaggca
     5221 cgagaggcgg ggcaccgatg gaactgaacc ttgatcgcga ggccgccatc ctcgagacga
     5281 tactcttcct tgagtccgag cctgtcgaca aggcagcgct ctcgcggata agcggcctgg
     5341 gtatcgacgt gacggaggcg gccctcgacc acctgcgcga atgcctggct tcggcctgtc
     5401 acggcatcga actcgtcgag atgggcggcg gctggctcct cgtcccgaag cgggacctat
     5461 gggaggggct caaggaccgc tacggccgca agaacgagca gaagctgtct agggcggctc
     5521 tcgagaccct gtcgatcatc gcgtactccc aaccagtgac cagggctgag gtcgaggcca
     5581 tacgcggcgt agcggcggac accatgatcc gcttcctgct cgacaagggc tttatcaagg
     5641 aagtcggcaa gaaggacgcg ccgggaaagc ccatccagta cggcaccacg aaggatttcc
     5701 tcaagttctt cagactcggc agcatctccg acctgcccaa gctcgacgag ttggagcgcg
     5761 atcgcttcga actcgaggga cgggcgtgag ctcgggcgag gacggtacca ggatacaggc
     5821 gctcattgcc cgcgccggcc tggcctcgcg ccgggccgcc gaggaatgta tgcgcgccgg
     5881 cagggtccgg ctgaacggcc gcgtggtcac cgagcagggc cggcgcgtcg accccgactc
     5941 ggatgcgatc cgcgtcgacg gcgcccggat cccgccgccg cgccgccacg cctacgtggt
     6001 gttcaacaag ccgcgcggcg tggtgtcgac gatggacgac cccgaaggcc gccccacaat
     6061 cagcgactac ctcggcaagc actcccgcga gcggctgtac cacgtcggac gcctcgacac
//
LOCUS       DAOPHV010000084         6106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_16874_length_6106_cov_4.483391, whole genome shotgun sequence.
ACCESSION   DAOPHV010000084 DAOPHV010000000
VERSION     DAOPHV010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16874_length_6106_cov_4.483391"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1505
                     /gene="der"
                     /locus_tag="PLQ29_03500"
     CDS             117..1505
                     /gene="der"
                     /locus_tag="PLQ29_03500"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPG85743.1"
                     /translation="MNEETTIRPSRTRWSGLPVVAIAGRPNVGKSTLFNRLLRSRRAI
                     VDPTPGVTRDPIEGEWLPDGADMPVTLVDTGGLKLDRDDMDDRVAKKSWERVASADLV
                     LFLLDAVDITPEDEEFASMLRRYAGKTVLVVNKADSPERDAAAWAHASWGYKDMVFIS
                     AEHGRNIDELAALIVSRLDWSGAKAEDEEHDDVRLAIMGKPNVGKSTLLNKLLGEERS
                     IVSDMPGTTRDVVEGRFEWNGRAFTVLDTAGMRRKAKVTEAVEYYSVNRAVKTLDRAD
                     VVVLMIDASEGLSDQDKKIVKLATDKGRAVLFALNKWDEMPKIKNAFEAARDKLQYFF
                     AQMAYAPVLPLSAKRGEGVDKLLKTAVELFDQLNTRVETSRLNKAVREWVESTPPPAH
                     PRARFKLRYAVQTGVNPVSFAIFATKPEIVGDSYRSYLKNRIRSDLGFKNVPIELELR
                     ASRKRFEDLEKD"
     gene            1509..1865
                     /locus_tag="PLQ29_03505"
     CDS             1509..1865
                     /locus_tag="PLQ29_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPG85744.1"
                     /translation="MSGPYRTGDVERLLGLGEHVLRYWERELPILSPSRSPFGRREWS
                     EADIALLLRVRHLVKVRGLGLSATMDALIAERSGDGAGAAAALSEARAALVSAYFDSR
                     SLAERLATIRHSSKHE"
     gene            1880..3211
                     /locus_tag="PLQ29_03510"
     CDS             1880..3211
                     /locus_tag="PLQ29_03510"
                     /inference="COORDINATES: protein motif:HMM:NF002759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HPG85745.1"
                     /translation="MPALNTKALCDFIDASPTPYHAVDSAAAMLNAVGAVHLDERDAW
                     SLEVGRAYYVSRDGASLIAFRVGRTAPSESGFALAGAHADAPGLRARIEKSVAAKGMD
                     RVAVEAYGGPIHATWLDRPLSLAGRVAVRTPDGIHRRLVNFARPVAVIPNLAIHFNRE
                     MNKGVEYPMNQALLPLVSAAGPQPAGADGQAQVGSWVLRAVAAELGVEPDAIVSAELT
                     FVDAAASAAIGADSEIVSAPRIDNLEGCHAVLSAFVSAPTGGHGQIAALFDNEEIGSA
                     TPRGADSSFLRDIIERIVALTDDSGPEAAHRALARSFSVSVDGAQAWHPAYADRFDED
                     FAPVLNGGPAIKANANVRYATDAIGEAAFRACCEAAGVPCQRFRMRADLSPGTTIGPI
                     SSALLGVRTVDVGVPMLAMHSARETAGAYDHDHMIAALGRFYAIGPSIADC"
     gene            complement(3298..3993)
                     /locus_tag="PLQ29_03515"
     CDS             complement(3298..3993)
                     /locus_tag="PLQ29_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85746.1"
                     /translation="MKRFVISFIALAMMAASVQAQSLADNSYYRKSVELEAAARAAFD
                     EGEYDEAAELAALAQENARLSDEYVAMILAMRKAKTAIESAQARYDWATGVRAELRFA
                     ADYAGATTELAAARAAFQAEAYAEALVHAYTVDAYLAGITDEEALPAYFVVRELSRRA
                     DCLWRIAGLPFVYNDPYKWPVLYEANKRSLPDPANPDLILPGMTLVIPAIANELRDGV
                     WIDGKEYPIFGTE"
     gene            complement(3998..5155)
                     /locus_tag="PLQ29_03520"
     CDS             complement(3998..5155)
                     /locus_tag="PLQ29_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85747.1"
                     /translation="MKIRSLPLIGLAAALLLGACASKPPATPEPEPQIVEPVPEPAKE
                     PEISLEELAALHARVVALRKDAFELGLKDVMPSEYADAEARYVAGKTALDADDRPAAK
                     AELEAAEPLFARLVAEGGLKVAEGRKADALAARDRALWADAETLSPEALAAADAANAD
                     ADAALAAGDAKAAIAAYRAAILAYDAAEKRSTALSVKSTVDELEYGPMDPGNYELAGQ
                     KLAAIDSLLATDGEAARDAAEEALLRYRLVLAKGWELTAGKRREAAERYKLDAEAIKA
                     QVAVKSEYAEAKAVWDSAVAASSAGDNESAAPLYEQSEYMFEAVYQKAAEKRAAAEAA
                     IQAAREKNERSASVAQRCDEQLGVEPAAQAESQATDEAASADGTSTAGEEE"
     gene            complement(5359..>6106)
                     /locus_tag="PLQ29_03525"
     CDS             complement(5359..>6106)
                     /locus_tag="PLQ29_03525"
                     /inference="COORDINATES: protein motif:HMM:NF039585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HPG85748.1"
                     /translation="EILQADAQGKPVRKAVTMGRSLRYQHNLKIRQPLAAVHLVTKDA
                     RERAVLLEMEDIVRDELNVKEVVFREDEEELVEYQAKANFRALGKELGKDMKEGAALV
                     ERLEPRQIASLLAGSSLVVDIAGRQVELTRDKVDVRRVEKASLRVVNEGTLTVALDAE
                     ITQALLDEGNARDLVRGVQTLRKDSGLSVTDRIELSVHGSEALRRSFEAFGDFVSGET
                     LAVALRWEETPGMVDVEAGDDTWKIAIKKA"
BASE COUNT          935 a         2065 c         2055 g         1051 t
ORIGIN      
        1 gacggaggag tacctcgcga agcggctggc cgaccgggtg tcgcgggagg actagggcgg
       61 gagccccggc cgaaccctag tccaatccgc ccgttccggt gtatccttta ccgcacatga
      121 acgaagaaac gaccatcagg ccctcccgca cgcgttggag cggccttccc gtggtagcca
      181 tagccgggcg cccgaacgtc ggcaagtcga cgctgttcaa ccgactcctc cgctcgaggc
      241 gggccatcgt cgacccgacg ccgggcgtca cgcgcgaccc catcgagggc gagtggctgc
      301 ccgacggcgc cgatatgccg gttaccctcg tcgatacggg cggcctcaag ctcgaccgag
      361 acgatatgga cgaccgcgtg gccaagaaga gctgggagcg cgtcgcctcg gccgacctcg
      421 tcttgttcct gctcgacgcc gtcgatatca ctcccgagga cgaggagttc gcgtcgatgc
      481 tcaggcgcta cgccggcaag acggtgctcg tcgtcaacaa ggcggacagc cccgagcgcg
      541 acgcggccgc ctgggcccac gcgtcgtggg gctacaagga catggtcttc atctccgcgg
      601 agcacggtcg caatatcgac gagctcgcgg cgttgatcgt ctcgaggctc gactggtccg
      661 gcgccaaggc cgaggacgag gagcacgacg acgtgcgcct cgccatcatg ggcaagccga
      721 acgtcggcaa gtccacgttg ctcaacaagt tgctcggcga ggagcgttcc atcgtcagcg
      781 acatgcccgg cacgacccgc gatgtggtcg agggccgttt cgagtggaac ggcagggcct
      841 tcaccgtgct cgacaccgcc ggcatgcggc gcaaggccaa ggtgacggag gccgtcgagt
      901 actactcggt caaccgcgcg gtgaagaccc tggaccgggc tgacgtcgtc gtgctgatga
      961 tagacgcctc cgagggcttg tcggaccagg ataagaagat agtgaagctg gccaccgaca
     1021 agggccgggc cgtcctgttc gccctcaata agtgggatga gatgcctaag atcaagaacg
     1081 ccttcgaggc cgcccgcgac aaactgcagt atttcttcgc gcagatggcc tacgcgccgg
     1141 tgctacccct gtcggccaag cgcggcgagg gcgtcgacaa gctgctcaag acggccgtcg
     1201 agctgttcga ccagctcaat acgcgcgtcg agacgtcgag gctcaacaag gccgtgcgcg
     1261 agtgggtgga gtccacgcct ccgcccgctc acccgcgcgc ccgcttcaag ctgcgttacg
     1321 cggtacagac cggagtcaac ccggtctcgt tcgccatctt cgccacgaag ccggagatag
     1381 tcggcgactc gtaccgatcg tacttgaaga accgtattcg ctccgacctc ggcttcaaga
     1441 acgtgccgat agagctcgag ctccgggcgt cacgcaagcg cttcgaggac ctggagaagg
     1501 actaggccgt gagcgggccg taccgtacgg gcgacgtcga acggctcctc gggctcgggg
     1561 agcacgtgct ccgctactgg gagcgcgagc tgcccatact gtcgccgtcc cgctcgccgt
     1621 tcggccggcg cgaatggtcc gaggccgaca tagcgctgct cctacgggtc aggcatctgg
     1681 tcaaggtccg ggggcttggg ctctcggcga cgatggacgc cttgatagcc gagcgctccg
     1741 gcgacggagc cggagccgct gcggccctgt ccgaggcccg ggccgccctc gtctcggcgt
     1801 atttcgattc gcggtcgctc gccgagcggc tggcgactat acggcatagc tcgaaacacg
     1861 aataatctgg aggtaatccg tgccggcact gaacaccaag gccttatgcg acttcatcga
     1921 cgcgtcgccg acgccgtatc acgccgtcga ctcggcggca gccatgctca acgccgtcgg
     1981 ggccgtccac ctcgacgagc gcgacgcgtg gagcctcgaa gtggggcgag cctactacgt
     2041 ttcgcgggat ggggcgtcgc tgatagcctt cagggttggg cgcaccgctc cgtccgagtc
     2101 cggcttcgcc ctggccggcg cccacgccga cgccccgggc ctgcgagccc gcatcgagaa
     2161 gtcggtggcc gcgaagggca tggatcgagt cgccgtcgag gcctacggcg gaccgataca
     2221 cgctacttgg ctcgataggc cgctgtcgct ggccggtcgc gtggccgtcc ggacgccgga
     2281 cggcatccac cgccgcctgg tgaacttcgc gaggcctgtc gccgtgattc ccaatctggc
     2341 catccacttc aaccgcgaga tgaacaaggg cgtcgagtac ccgatgaacc aggcgctgtt
     2401 accgctcgta tcggccgccg gacctcaacc ggccggggcc gacggccagg ctcaggtcgg
     2461 ctcatgggtc ctacgcgccg tggcggccga gctcggcgtc gagccggacg ccatagtctc
     2521 cgccgagctg accttcgtcg acgcggccgc ctcggcggct atcggcgccg actctgagat
     2581 cgtatcggcg ccgcgtatcg ataatctcga gggctgccac gcggtgctgt ccgccttcgt
     2641 atccgccccg actggcgggc acggtcagat cgcggccctc ttcgataacg aggagatagg
     2701 ctcggccacg ccgcgcggcg ccgattcgtc gttcctgcgg gatattatcg agcgcatcgt
     2761 ggcgctgacc gacgattccg gccccgaggc cgcccatagg gcgctcgcca ggtcgttcag
     2821 cgtgtcggtc gacggcgcgc aggcctggca tcccgcctac gccgatcgtt tcgacgagga
     2881 tttcgcgccc gtattgaacg gcggcccggc cataaaggcc aacgccaacg tccgctacgc
     2941 caccgacgcg atcggcgaag ccgccttcag ggcttgctgc gaggccgcgg gcgtcccgtg
     3001 ccagcgtttc aggatgcgcg ccgacctatc gcctgggacg acgataggac ctatcagctc
     3061 ggccctgctc ggcgtacgca ccgtcgacgt cggcgtgccg atgctcgcga tgcactcggc
     3121 ccgcgagacg gccggcgcct acgaccacga ccatatgata gccgccctcg gccgtttcta
     3181 cgcaatcggg ccttcgatcg cggattgcta ggtccgttcg acgaggcgct acacaagggc
     3241 cgcccgatcg ggcggccctt gtgtcatcca ggcgaccgta tcgctgacga acgaatacta
     3301 ttcggttccg aagatcggat actccttgcc gtcgatccag acgccatccc gtagctcgtt
     3361 cgctatcgcc ggtatgacca gggtcatgcc tggcaggatc aggtcggggt tggccggatc
     3421 gggcagagag cgcttattgg cctcgtagag cacgggccac ttgtacgggt cgttgtagac
     3481 gaagggtagg ccggctatac gccacaggca gtccgcgcgc ctggagagct cgcgtaccac
     3541 gaagtaggcg ggtagggctt cctcgtcggt aatgcccgcc aggtaggcgt ctacggtata
     3601 cgcatggacg agagcctcgg cgtaggcttc ggcctggaag gcggcgcggg cggcggcgag
     3661 ctcggtcgtg gcgccggcgt aatccgctgc gaagcgcagc tcggcgcgga cgccggtcgc
     3721 ccagtcgtag cgcgcctggg ccgattcgat cgcggtcttg gccttgcgca tcgccaggat
     3781 catggcgacg tactcgtcgg atagcctggc attctcctgg gccagcgccg ccagctcggc
     3841 ggcctcgtcg tactcgccct cgtcgaaggc ggcgcgggcc gcagcctcga gctcgacgct
     3901 cttgcggtag taggagttat cggccaggct ctgggcctgg accgaggccg ccatcatggc
     3961 gagggctatg aagctgatga cgaatctctt catgtcgtta ctcctcctcg cctgctgtag
     4021 acgtgccatc ggcgctcgcg gcctcatccg tcgcctgact ctcggcttgc gccgcgggtt
     4081 cgacgccgag ctgttcgtcg caccgctggg ctacggaggc gctgcgctcg ttcttctccc
     4141 gggcggcctg gatggccgcc tcggcggccg cgcgcttctc tgcggccttc tggtatacgg
     4201 cctcgaacat gtactctgac tgctcgtaca gcggggccgc cgactcgttg tcgccggccg
     4261 aggaggccgc gacggccgag tcccagacgg ccttggcctc ggcgtactcg gacttgacgg
     4321 cgacctgggc cttgatggcc tcggcgtcga gcttgtagcg ctcggccgcc tcgcgccgct
     4381 tacccgccgt cagttcccag cccttggcaa gtacgagcct atagcgcagc agcgcctcct
     4441 cggcggcgtc tcgggcggcc tcgccgtcgg tcgccaggag cgagtcgata gcggccagct
     4501 tctgcccggc tagctcgtag ttgcccgggt ccatcgggcc gtactcgagc tcgtcgacgg
     4561 tcgacttgac cgacagcgcg gtcgaacgct tctcggcggc gtcgtaggcg agtatggcgg
     4621 cgcggtacgc ggcgatggcc gccttcgcgt cgcccgcggc gagcgcggcg tcggcgtcgg
     4681 cgttcgcggc gtcggccgcg gccagggcct cgggcgacag cgtctcggcg tcggcccaca
     4741 gggcgcggtc gcgggccgcc agggcgtcgg ccttgcggcc ctcggcgacc ttgaggccgc
     4801 cctcggccac gagccttgcg aatagcggct cggcggcctc gagctccgcc ttggccgccg
     4861 gcctgtcgtc ggcgtcgagc gccgtcttgc cggccacgta gcgggcctcg gcgtctgcgt
     4921 attcggacgg catcacgtcc ttgagcccta gctcgaaggc gtccttcctg agcgctacga
     4981 cgcgcgcgtg gagagccgct agctcctcga gcgatatctc gggttccttg gccggctctg
     5041 gaaccggctc gacgatttgc ggttcaggct ccggcgtagc gggcggtttc gaggcgcaag
     5101 ccccgagcag cagcgccgcg gcaaggccga tgagcggtag cgaacgtatt ttcattggta
     5161 cctccattat gcggacggga tgtcggcgat cgactgagaa cggtacggat cgtatcgcct
     5221 tgcgatggat ggtcaagact ctatggtagt ataacagtag cacgtctggg gttttagggc
     5281 aaaccatata agccccaatc aaataaaagg ccgcccctcg gggcggccgg atacggcgat
     5341 tcacggctcg aagcgtgctt acgccttctt gatcgctatc ttccaagtat cgtcgcccgc
     5401 ctccacgtcg accatgcccg gcgtctcctc ccagcgcagc gcgacggcga gggtctcgcc
     5461 ggagacgaag tcgccgaagg cctcgaacga gcgcctgagc gcctcgctgc cgtggacgga
     5521 cagctcgatg cggtcggtga ccgacaaacc ggagtccttc ctgagcgtct ggacgccgcg
     5581 gacgaggtcg cgggcgttgc cctcgtcgag cagggcctgg gtgatctcgg catcgagggc
     5641 gaccgtcaac gtaccctcgt tgacgacgcg taggctggcc ttctcgacgc ggcgtacgtc
     5701 gaccttgtcg cgcgtcagct cgacctgcct gccggctatg tcgacgacga gcgacgaccc
     5761 ggccaggagg ctggcgatct gcctgggctc gagccgctcg accagggcgg ctccttcctt
     5821 catgtccttg ccgagctcct tgccgagcgc ccggaagttg gccttggcct ggtactcgac
     5881 cagctcctcc tcgtcctcgc ggaagacgac ctccttgacg ttgagctcgt cgcggacgat
     5941 gtcctccatc tcgaggagca ccgcccgctc gcgggcgtcc ttggtgacga ggtgcaccgc
     6001 cgcgagcggc tgccgtattt tgaggttgtg ctggtagcgc agggatcggc ccatcgtcac
     6061 ggccttgcgc accggcttgc cttgggcgtc agcctgcaga atctcg
//
LOCUS       DAOPHV010000085         6057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_17096_length_6057_cov_3.843719, whole genome shotgun sequence.
ACCESSION   DAOPHV010000085 DAOPHV010000000
VERSION     DAOPHV010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17096_length_6057_cov_3.843719"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PLQ29_03530"
     CDS             <1..590
                     /locus_tag="PLQ29_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPG85749.1"
                     /translation="FSPPRVRPHLHLSVQSGSDRVLAAMGRRYRRDAVLRAAEAARAA
                     RGDPFIGADIILGFPGEGDDDFADTVDLLERLEPAWVHAFTFSPRPGTRAFDMRPKVP
                     ERVAVERAAAVQAVAERGKAAFAARRAGRVVDAVIESPAESGEPALATSAEYLKLAIR
                     GAPTGFVGACSCRVDPEPPTEGLRDGLDLTASFIA"
     gene            700..1470
                     /locus_tag="PLQ29_03535"
     CDS             700..1470
                     /locus_tag="PLQ29_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85750.1"
                     /translation="MKRTIMIVLAIAVALSMTACSALDALLSVNVFAPFAGVSSGAIE
                     KATPDELITMSGSESFYETLAAKPELKETILKTIDKAMAEAPASSATYQELAVLGANI
                     ELQTSPAGDLINNIGGLFGSLASGDGGEGEDVDFGDMIAGLIPPEVKNADGSLDKEAF
                     VAMIDALVAADAYYERLGAAISAGDVAQAALVSAIIAAIPTPVGYTTGQYLYDLQNDV
                     PGTPEMAEFSMPDMTTGSLYNILAAAGLGELFASQTGE"
     gene            1481..2626
                     /locus_tag="PLQ29_03540"
     CDS             1481..2626
                     /locus_tag="PLQ29_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85751.1"
                     /translation="MKRVIVLAMIALLVGGAAFAQTTLSNFYPKDARSMAMGGAFVAM
                     SDGFQSLYGNPAAFADKKAELTLLSMTPWLYLSPTTENIDAVSGLVSGEGDEQDMFNT
                     LTGLVTKNGFGAGASLGLGWVGKGLGLGLVGGADAYLSGKTLLGATGSVDGQMNAVIG
                     IGVPLNLGPFRLQVGGDVRPYLRMTGDIVGTQLIGMIVGGDDFDPMTLPVDVGFGLAV
                     DLGATLELGKLVSVGLAIRDLSTKQTYARSTIGEVLESEGLPSGTEATYAVMPNITVG
                     AAVTPIPAFLRPLIDVTVIAELQDPIKVIRDNSSMWNLFHLGAEAETLGGLIAVRAGL
                     NKGWLSFGAGLDLLIMEVNVALFTEELGPYPGDRGRTGVSAEVAIRF"
     gene            2680..3420
                     /locus_tag="PLQ29_03545"
     CDS             2680..3420
                     /locus_tag="PLQ29_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85752.1"
                     /translation="MHPTRTRAAALAACLASLALASSCQQFFTTSLAPFLARVSYTIP
                     ADLPVEDAIRLLEQAMAQGDSSAAAALVTPLLAAATAAAEADPESAAYHQAASALLDA
                     AVLSSGVSSAMTTLASGYLALGDEEPTGEQLATMMAAFSSVSLDDTAEDALLLLAAYP
                     PSDMSSEEAYAAGLALMADGLSDAGSSLADMESLTEEQLAALEEDPSFAAAMMFLLYG
                     AAIDEANGETSLVGGLLGGMLPEVEPEP"
     gene            3467..4603
                     /locus_tag="PLQ29_03550"
     CDS             3467..4603
                     /locus_tag="PLQ29_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85753.1"
                     /translation="MKRYALLCAAVVAMALSSHAQGVASFSMPSARMSALGGPHAASV
                     SGVDAIFENPAGFVAEETEFSFGAIVVNPAGPVFDMAGLMLSGGDLLTGAAGLFDDSG
                     RLYFQADLLGPLSFAYAGKGLGFGLFNRTVATVNAASLFNVAYSASEEIMLAGGYAYR
                     FALGDGHTIDLGIMPKGFIRASVGDVASLTDIMALVSDPGSLLASPLTMVSGVGFDAG
                     VQWTYRRSLTVGFVAHDAYSPAMVTTYDSYEAFADSPSSGVSAYAVVPAVLSVGAAYD
                     VPWPLLGRLGADLLVLVDYTDLLDLFSALPRNPILNVSFGAELTLLDILSLRAGVKDA
                     LPTAGFGIDLSVFTFSLAMYGEELGLEPGSRPVFNLLVAFEFKY"
     gene            4667..4739
                     /locus_tag="PLQ29_03555"
     tRNA            4667..4739
                     /locus_tag="PLQ29_03555"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4700..4702,aa:Lys,seq:ttt)
     gene            complement(5221..5883)
                     /locus_tag="PLQ29_03560"
     CDS             complement(5221..5883)
                     /locus_tag="PLQ29_03560"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPG85754.1"
                     /translation="MNAMPASPIGTSSIFGDADGAGVWSRCLDAAIEAFNSGSLGIAA
                     AVVDPGMAIVSLGRNRMDEIPDDPLSISGSSVSHAETNAIHGLGRRSLGDYELSLYTT
                     VEPCPMCVGAIGMSRIRRVVVASRDPHAGSLDLLESSGYLRSKGITTEEPGGAIERVF
                     FGIHYLSIKRLPHIAPDHRIFAAFRTRYGGFMEEIDDGLGTAPLATLRLTYEAIDRLF
                     GP"
BASE COUNT          969 a         2127 c         1920 g         1041 t
ORIGIN      
        1 ctttctcgcc gccgcgcgtg cggccgcatc tgcatctgtc cgtgcaatcc ggctccgacc
       61 gcgtgctcgc ggccatgggc cggcgctacc gccgcgacgc cgtgctgcgc gccgccgagg
      121 ccgcccgggc cgcccgcggc gaccccttca tcggcgcgga catcatcctc ggcttcccgg
      181 gcgagggcga cgacgacttc gccgacaccg tcgacttgct cgagcgcctc gaaccggcct
      241 gggtgcacgc cttcaccttc agcccgcgcc ccggcacccg cgccttcgac atgcggccca
      301 aggtgcccga gcgcgtcgcc gtcgagcgag ccgccgccgt gcaggccgtc gccgagcgcg
      361 gcaaggccgc cttcgccgcc aggcgcgccg gccgcgtcgt cgacgccgtc atcgagtcgc
      421 ccgcggagtc cggcgagccg gccctggcca cctcggccga gtacctgaag ctcgccatcc
      481 gcggcgcgcc gacgggcttc gtcggcgcgt gctcctgccg cgtcgacccg gagcctccga
      541 ccgaagggct acgggatggc ctcgatctta cggccagctt catcgcttag taatggagcg
      601 aatggattaa tttatataaa tctcacttgt tttcttagcg ctaagtgcta cacttacgtt
      661 tagatgaccg tcgccagatc gacggtaagg aggactgcga tgaaacggac catcatgatc
      721 gtcctggcca tcgccgtggc gctgtcgatg acggcctgca gcgcgctgga tgcattgtta
      781 tcggtaaatg tattcgcccc gttcgccggc gtcagttcag gcgccatcga gaaggccacc
      841 ccggacgagc ttataaccat gtccggtagc gaatccttct acgagacgct cgcggccaag
      901 ccggaactca aggagacgat ccttaagact atcgacaagg ccatggccga agcgcctgcc
      961 agctccgcca cgtaccagga gctcgccgtg ctcggggcca atatcgagct ccagacctcg
     1021 ccggccggcg acctgatcaa taatatcggc ggcctgttcg gtagcctcgc ttccggcgac
     1081 ggcggtgagg gcgaagatgt cgacttcggc gatatgatcg ccggcctgat accgcccgag
     1141 gtcaagaacg ccgacggcag cctcgacaag gaggccttcg tcgccatgat cgacgcgctc
     1201 gtggcggccg acgcctacta cgagcggctc ggcgccgcta tatccgccgg cgatgtcgcc
     1261 caggccgccc tcgtgtcggc gatcatcgcc gccatcccga ctcccgtggg ctatactacc
     1321 ggccaatacc tctacgacct gcagaatgac gtgcctggta cccctgagat ggcagaattc
     1381 tcgatgcctg atatgacgac cgggtcctta tacaatatcc tcgccgcggc cggcctcggc
     1441 gagctgttcg cctctcaaac cggcgagtaa ggaggacgac atgaaacgcg ttatagtact
     1501 ggcgatgatc gccctcctcg tcgggggcgc ggccttcgcc cagaccaccc tgagcaactt
     1561 ctatcccaag gacgcccgta gcatggcgat gggcggcgcc ttcgtcgcga tgagcgacgg
     1621 cttccagagc ctctacggca accccgcagc cttcgccgat aagaaggccg agctgaccct
     1681 gctgtcgatg actccctggc tgtacctgag cccgaccacc gagaatatcg acgcggtctc
     1741 cggcctcgtc tcgggcgagg gcgacgagca ggacatgttc aataccctga ccggcctcgt
     1801 caccaagaac ggcttcggcg ccggcgccag cctaggcctc ggctgggtag gcaagggcct
     1861 cggcctcggc ctcgtcggcg gcgcggacgc ctacctgagc ggcaagaccc tgctcggcgc
     1921 caccggctcg gtcgacggcc agatgaacgc cgtgataggc atcggcgtac cgctcaacct
     1981 gggaccgttc aggctgcagg tcggcggcga cgtccgaccc tacctgcgca tgaccggcga
     2041 catcgtcggt acccagctga tcggcatgat agtcggcggc gatgatttcg acccgatgac
     2101 cctgccggtg gacgtcggct tcggcctcgc ggtcgacctc ggcgctaccc tcgagctcgg
     2161 caagctggta tcggtaggcc tcgcgatccg cgacctgtcg accaagcaga cctacgcccg
     2221 ttcgaccatc ggcgaggtac tcgagtcgga gggactgccc tccggtaccg aggcgaccta
     2281 cgcggtgatg ccgaacatca ccgtcggcgc cgccgtcacg cccatcccgg ccttcctccg
     2341 gccgctgatc gacgtgaccg ttatcgccga gctccaggac ccgatcaagg tgatccgtga
     2401 caactcctcg atgtggaacc tgttccacct cggcgccgag gccgagaccc tcggcgggct
     2461 catcgccgtc cgagccggcc tcaacaaggg ctggctgagc ttcggcgccg gactcgacct
     2521 cctgatcatg gaggtcaacg tcgcgctgtt caccgaggag ctcgggccgt atccgggaga
     2581 ccgcggtcgg accggcgtct cggccgaggt ggccatacgg ttctgatgaa aaccggtcgc
     2641 cgcgccgata ctagtactac gacgaaagga gcggcgacca tgcatccgac tagaacgagg
     2701 gcggcggccc tggccgcctg cctcgcgagc ctcgcgctcg cctcatcctg ccagcaattc
     2761 ttcaccacgt ccctggcccc cttcctggcc agggtttcgt ataccatacc ggccgacctg
     2821 cccgtcgagg acgccatccg cttgctcgaa caggcgatgg cccagggcga ctcgtcggcc
     2881 gcggccgccc tcgtaacgcc gctcctggcc gccgctacgg ccgctgcgga agccgacccg
     2941 gaaagcgcgg cctaccatca ggcggcctcg gcgctgctcg acgcggccgt gctgtcgtcg
     3001 ggcgttagct ccgcgatgac cacgctcgcc tccggctacc tggccttggg cgacgaagag
     3061 ccgaccgggg aacagctggc gacgatgatg gccgctttct cgtcggtatc gctcgacgat
     3121 acggccgagg acgccctgct gctcctggca gcctatcctc cctccgacat gagttccgag
     3181 gaggcctacg ccgccggcct cgccctgatg gccgacgggc tgtccgacgc gggctcgtcc
     3241 ctggcggata tggagagcct gaccgaggaa cagctcgccg cgctcgaaga agatccctcc
     3301 ttcgccgccg ccatgatgtt cctgctctac ggcgccgcca tcgacgaggc gaatggcgag
     3361 acgagcctcg ttggcggact cttgggcggc atgttgcccg aggtcgagcc ggagccctaa
     3421 tcggccgata ggtatacagt atgaaaccga tcagcgaggt ccgccgatga aacgatacgc
     3481 ccttctatgc gccgccgtcg tcgcgatggc gttgtcgtcg cacgcccagg gcgtggcatc
     3541 cttctcgatg ccctcggcca ggatgagcgc cctgggcggc ccgcacgccg ccagcgtctc
     3601 cggcgtcgac gcgatcttcg agaatccggc cggcttcgtc gccgaggaga ccgaattcag
     3661 cttcggcgcc atcgtcgtca acccggccgg acccgtcttc gacatggccg gcctgatgct
     3721 ctccggcggg gacctcctga ccggagcggc cgggctgttc gacgactcgg gccgcctgta
     3781 cttccaggcg gacctgctcg ggccgctgtc cttcgcctat gccggtaagg ggctcggctt
     3841 cggcctgttc aaccggaccg tcgccaccgt caacgcggcc tctctcttca acgtcgccta
     3901 ctccgcctcc gaggagatca tgctcgccgg aggctacgcc taccgcttcg cccttggcga
     3961 cggccatacg atagacctcg gcatcatgcc caagggcttc atccgcgcca gcgtcggcga
     4021 tgtcgcgtcc ctgaccgaca tcatggccct cgtctccgac cccggctcgc tgctcgcgag
     4081 cccgctgacg atggtgtcag gcgtcggctt cgacgccggc gtccagtgga cctaccgccg
     4141 ctcgctgacg gtcggtttcg tggcccacga cgcgtactcg ccggccatgg tcaccacata
     4201 tgacagctac gaggcgttcg ccgacagccc gagctccggc gtgtcggcct atgcggtcgt
     4261 gccggccgtg ctgtccgtcg gcgccgccta cgacgtgccc tggccgctac taggccgcct
     4321 cggcgccgac ctcctcgtgc tcgtcgatta caccgacctc ctcgacctgt tctccgcgtt
     4381 gccgaggaac ccgatcctga acgtctcgtt cggcgcggag ctgaccctgc tcgacatcct
     4441 gtcgctacgg gccggcgtca aggacgcgct cccgacggcc ggcttcggta tcgacctgtc
     4501 ggtctttacc ttctcgctcg cgatgtacgg cgaggaactc ggcctcgagc caggtagccg
     4561 gcccgtgttc aacctgctcg tcgccttcga gttcaagtac tgacgcgccc ctcgccgcgg
     4621 cgttgacacg ttgccgcgcc ggagggtatg gtcagggtcg ccccacgagc cgctagctca
     4681 ctcggtagag caacgccctt ttaaggcgtg ggtaccgggt tcgaaccccg ggcggctcat
     4741 cgtcacccat gacgctcctg tccttcggac aggcaattct gtggcgcgcc ctccggatat
     4801 gcttcgcata tccggattcc gcaaggaagc gattgacgtg gccgctgaaa gcggcctgct
     4861 ccaaccttgg cgcgctatcg tatcagcact attgcggcgt gtatctgcta ccgaactccg
     4921 gtgatcatcc cggggttcgc atcgcacgtc gcgcaagcga cgcttttcaa agcaattcct
     4981 tgcgatgccg cgccgaaagg cgcggtaggg ttcgagcgcg gcgcgaatgc gctgaaaaac
     5041 gcgatggagc gtgcaggagg gcgctgtaag cgcgagactg gaggcgcgaa cggatcgtag
     5101 ggagggggat accccctcga gcggcggtat accgccgctc tcacccctgc gatcctgcgc
     5161 accgatatgg tatagctgcg gtgcgtacca gctgtaaccg acccataatc ttcataacac
     5221 tcaagggccg aacagcctgt cgatcgcttc ataggtcagt cggagcgtcg ccaatggcgc
     5281 ggttccgagc ccgtcgtcga tctcttccat gaacccgccg tatcgggtcc ggaaagcggc
     5341 gaagattcga tggtcgggcg cgatatgggg caggcgcttg atggataggt aatggatgcc
     5401 gaagaacacc cgctcgatag ctccgcccgg ctcttcggtc gtgatgccct tggaacgcag
     5461 atagccgctc gactcgagca ggtcgaggct gccggcgtgg gggtcgcgcg aggcgacgac
     5521 gacgcggcgt atcctggaca tgccgatggc gccgacgcac atcgggcagg gctcgaccgt
     5581 ggtgtagagg gacagctcgt agtcgccgag gctcctgcgg ccgaggccgt ggatggcgtt
     5641 ggtctcggcg tgcgagacgg agctgccgct tatggagagc ggatcgtcgg gaatctcgtc
     5701 catccggttc cgcccgaggc tgacgatggc cattcccgga tcgacgacgg ccgccgctat
     5761 gccgagcgat cctgagttga acgcttcgat cgcggcgtcg aggcaccggc tccatacgcc
     5821 ggcgccgtcg gcgtcgccga agatcgacga cgtccctatc ggagacgcgg gcatcgcgtt
     5881 cataacgaga ctggcgctac tcggcggcct gctcgatagc cgcgccggcc tgcggcagcc
     5941 ggaccacggc gccgaggctc gcgctgaaca tgaacgccga cgaggccggt ccgcggccgg
     6001 ccaggctctc gatgccgaat acgtagacgc ccgagtcgag gccggcgacg agggtca
//
LOCUS       DAOPHV010000086         6032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_17187_length_6032_cov_4.486866, whole genome shotgun sequence.
ACCESSION   DAOPHV010000086 DAOPHV010000000
VERSION     DAOPHV010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17187_length_6032_cov_4.486866"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLQ29_03565"
     CDS             <1..685
                     /locus_tag="PLQ29_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85755.1"
                     /translation="APPKGRSSMRRGVSVALAAVVAVVASSSSCGRGPAPATRPSLAA
                     SIKVARGNRLFAEGFYHEAAALYLEAGAGSDPTASYDLANVFSALGEGEAASSMRARA
                     VALGEADGYSGLQASAWFNEGVDAYAAGAYDEAAAAFRKALAEASSVGASVGAFGLGP
                     VARLELSRAYELALAALADKRESSASERGAYEAAQVPGESRPLSLSRIDERTLFAPGS
                     SESAGGEDH"
     gene            685..2520
                     /locus_tag="PLQ29_03570"
     CDS             685..2520
                     /locus_tag="PLQ29_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85756.1"
                     /translation="MRASMRAAAALLCLLAGAAGWAQDERRVAISPDRPAVGSSFVIE
                     AFLPGERAEALEAIEPDILGPARFSGADVRPAQTSGGGPAATITYRFVVEGAGRIDVL
                     GLIAVSGTRTLSLGSWSIETESLDRGPVARIGSWSAPASVYEREVFVIEARGPDGSPV
                     ACPALAVEGLLLDPIPGRTGAYYAVAPAAGAARLPSLRLDDGYGPFELGERPVRMRSL
                     PAEAEGVMAVGGPWRLELSFPGQAGSADKLSFSSTSFSSTSLGSGDTLSWELRAVGSG
                     WPGLAGWPRLTIRAEGIGERRVAEAVRYVERGKGRLDYVAGARGAFAAAEPGVYVIEP
                     EPYAWFDPVAQAVRYAAASAVRVVVTERVSPPWSPPAAVAELARSAASRLARRDDAWV
                     GVAAAVSAAGSPDWGAALDEATRAAASDGGTPRERAALAALSLLADTGSERDRAAAYA
                     ELKRLGRAACILPGLPAALAALDESFGNLDDGADVLPPFGYPAAGAVVLSAAAIAAIL
                     RRRASDRRASGHGSRRRLSLPLGLAVAGAALAAFAALAAAEEARPRFVCLGGVARTVP
                     SAEAGGSAMAAGRGGRVLESAGGWLFVEPAGGSAGWLAADDAVVY"
     gene            complement(2941..3162)
                     /gene="infA"
                     /locus_tag="PLQ29_03575"
     CDS             complement(2941..3162)
                     /gene="infA"
                     /locus_tag="PLQ29_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPG85757.1"
                     /translation="MAKEEAIEVEGIVREALPNTMFRVELKNGGHLILAHLSGKMRKH
                     YIRIVPGDTVRVALSPYDLSRGRIIYRER"
     gene            3369..3725
                     /locus_tag="PLQ29_03580"
     CDS             3369..3725
                     /locus_tag="PLQ29_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HPG85758.1"
                     /translation="MDKDFTEKMRESLTEMKQDILDKLVATNADFRAIVEEMDPKDFA
                     DVASDDVDRKMIEALGSQDLKRLRSIEAALVRIQQGRYGSCMKCSKRIPQERLVALPY
                     AVLCIECQKSEERRNR"
     assembly_gap    3792..3891
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3896..4780)
                     /locus_tag="PLQ29_03585"
     CDS             complement(3896..4780)
                     /locus_tag="PLQ29_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HPG85759.1"
                     /translation="MLTNFHTHCEFCDGKATAAAMADAARRAGFSILGFSSHAPLPFV
                     TRWTMKQASLGDYVDAIRGLGRTHGPDMRILVGLEVDSIEGLCGPADGRFDGAGLDYR
                     IGSVHFVLPDGAPAPTPEALDETGEPTFGFTVDESEEKFDDHLSRFYDGDAEAMVDDY
                     YAAVAACARAGGFDILGHFDLVRKNNRGQRRFSEEGRRYRDAAMAAVEAVAESGAIVE
                     INTGGMARGKTDSPYPAAWILRELKARGVGVCLNADAHETRHLSAHREDGLRAAAAAG
                     YDRLTVIGPDGRFQVELG"
     gene            4930..>6032
                     /locus_tag="PLQ29_03590"
     CDS             4930..>6032
                     /locus_tag="PLQ29_03590"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HPG85760.1"
                     /translation="MALGAIHIRYGRLAHEQKPFKVPHTFVLLFALIIVAVIGSYIIP
                     AGEFDRATDPNSGRTVVVPGTFHRVEQNPQHPFKTFIALQKGSIDAADVVFFVFFVYA
                     SFYIVLQTGALHSFIGGLLRALRGKEVVLIPVFMYIFALGGSVFGMFEETFGFIPLFV
                     GLAIAMGYDAIVGMCMVFLAVGMGFAAAFMNPFTIGVAQKVAELPLFSGMGFRIVSWF
                     VFVTMAVIWTVRYALRIKKDPSKSHLASVDMGKLALNHDELVDKKATGRDRLILAWVV
                     GTIGVLIWGVIAKGWYFDELAGLFVIMGIATGLIADWGPSKIAATFLEGCKDIVFGAL
                     VVGLSRGILVVMREAQIIDTVVYAMSQPLAALPR"
BASE COUNT          871 a         1914 c         2102 g         1045 t
ORIGIN      
        1 cgctccgccg aaaggacggt cgtccatgag gcgcggcgtc agtgtcgcgc tagcggccgt
       61 cgtcgccgtg gtagcctcgt cgtcgtcctg cggtcgaggc ccggcccccg cgacgcggcc
      121 gtctctggca gcgtcgatca aggtggcgcg gggcaatagg ctattcgccg agggcttcta
      181 ccatgaggcc gcggccctgt acctggaggc cggggcaggc agcgatccga cggcctcgta
      241 cgacctggcc aacgtattct cggccctcgg cgagggcgag gcggcctcgt cgatgcgggc
      301 gcgggcggtt gcgctcggcg aggccgacgg ttactccggg ctacaggcca gcgcctggtt
      361 caacgagggc gtcgacgcct acgccgctgg cgcctatgac gaggcggcgg cggctttcag
      421 gaaggcgctg gccgaggcgt cctccgtcgg agcctctgtc ggcgccttcg gcctcgggcc
      481 ggtcgcgcgg ctcgaactat cgcgggccta cgaactggcc ctcgcggccc tggccgacaa
      541 gcgcgagtcg agcgcatccg agcgcggcgc ctacgaggcg gcgcaggtac cgggcgaatc
      601 gcggcctctg tccctgtcca ggatcgacga gcgtactctg ttcgccccgg gctcctccga
      661 gtccgccggc ggggaggacc attgatgcgc gcttcgatgc gggccgcggc ggctttgctg
      721 tgcctgctcg ccggcgcggc cgggtgggcc caggacgagc gtcgtgtggc catatcgccg
      781 gatcggccgg ccgtcggctc gtccttcgtc atcgaggcgt ttctgccggg cgagcgcgcc
      841 gaggcgctcg aggcgatcga accggatatt ctggggccgg ccaggttctc cggggcggac
      901 gtacggccgg cgcagacgtc cggcggcggg ccggccgcga ctattacgta tcgcttcgtg
      961 gtcgaaggcg caggccggat cgacgtgctg ggcctgatcg cggtatcggg gacccgtacc
     1021 ctgagcctcg ggtcttggtc gatcgagacc gagagcctcg atcgggggcc ggtcgcccga
     1081 atcggttcgt ggtcagcccc cgcttccgtc tacgagcgag aggtgttcgt gatcgaggcc
     1141 aggggccccg acggctctcc ggtagcctgt ccggcgctgg ccgtggaggg actgctgctc
     1201 gatccgatac ccggccggac gggcgcctac tacgcggtcg ctccggcagc cggggccgct
     1261 cggttgcctt cgctcaggct cgatgacggc tacggcccct tcgagctcgg tgagcgtccc
     1321 gtgaggatgc gttcgttgcc ggccgaggcc gagggcgtca tggccgtcgg cgggccgtgg
     1381 cggctggagc tttcgttccc ggggcaggcc ggctcggccg acaagctgag cttcagctcg
     1441 acgagcttca gctcgacgag cctaggctcg ggagatacgc tgtcctggga gctacgcgcc
     1501 gtaggctcgg gctggcccgg cctcgccggc tggccgaggc tgacgatacg ggcggagggg
     1561 atcggagagc gtcgcgtcgc ggaggcggta cggtacgtcg aacgcgggaa ggggcgcctc
     1621 gactacgtgg ccggcgcgcg tggcgccttc gcggccgccg agcccggcgt ctacgtgatc
     1681 gagccggagc cgtacgcctg gttcgacccc gtcgcccaag ccgtacgata cgccgccgcg
     1741 tcggctgtca gggtggtcgt gacggagcgc gtctcgccgc cgtggtcgcc gccggccgcg
     1801 gtcgccgagc tggcgaggtc cgccgcgtcg cggctggccc gtcgcgacga cgcctgggtc
     1861 ggggttgcgg ccgccgtctc ggcggcagga agtcccgact ggggcgcggc cctcgatgag
     1921 gcaactcgcg ccgcggcgtc cgacggcgga acgccgcgcg agcgcgcggc gctagccgcg
     1981 ctaagcctgc tggccgatac gggctccgag cgcgatcgcg ccgccgccta cgccgagctg
     2041 aaacggttgg ggagagcggc ctgtatccta ccggggcttc ccgcggccct ggcggcgctc
     2101 gacgagtcgt tcggtaacct cgacgacggc gcggatgttt taccgccgtt cgggtatccg
     2161 gcggcgggcg ctgtcgtcct gtcggcggcc gccatcgccg cgatactccg gcgtcgcgcc
     2221 tcggaccgcc gcgcctctgg tcacggctcg cgtcgtcggc tctcgttgcc gctcggcctg
     2281 gccgtcgcgg gagccgcgct ggctgccttc gccgccctgg ctgcggcgga agaggctcga
     2341 ccccgcttcg tctgcctcgg cggcgtagcg aggaccgtgc cgtcggccga ggccggtggc
     2401 tcggctatgg cagcgggccg cggcgggcgc gtgctcgagt cggccggcgg ctggctgttc
     2461 gtcgagccgg ccggcggttc ggccgggtgg ctggcggccg acgacgccgt ggtatattag
     2521 gccggcggga aggggcgcga ggtggacttc ggcgagatac tcaagttgtg ggacgcgaag
     2581 accggggccg ccgcgttagc cgaagccgga cgcggtgacg gtaaggacgg gccgaaggag
     2641 cgccgcctga ctaagaagga gctagacgcc ttgcccgtgg acgcgaggct cgacctccac
     2701 ggactgaccg ccgcggaggc ggaggacgcg ggcgtccttc ttcgcggcgg cggagcgcgg
     2761 aggctgtcgc aagctgatga tagtccacgg caagggcctg cattcggcgt cggagcccgt
     2821 gttggcgggc gtcgtacgcc gttggctgga acgcaggccc tcggccggca aatccggcca
     2881 cccggactcg gccaatggag ggactggagc tacctgggtt ctattgaagg aacgggagga
     2941 ttagcgctcg cgatagatga tgcgccctcg ggataggtcg tacggcgaca aggctacccg
     3001 gaccgtgtct ccggggacga tacggatgta gtgcttgcgc atcttgccgg aaaggtgggc
     3061 tagaatgagg tgtccgccat tctttaattc gaccctgaac atggtgttcg gcagggcttc
     3121 cctgacgata ccctctacct cgatagcttc ttccttggcc acgatgcctc ctacggagaa
     3181 atgggacgct ctcgcttgag cgcggcgtcc gaagcgctat aatagtacgg gccgcgcgtt
     3241 ggcgtgtcaa cctgcccgtc cggatggttg acagagacac gcaatccatg aatagtaact
     3301 cgctcgtttc tatgacgatc cggcgccgac ggcgcccacg ctgacgcttg agtatcgagg
     3361 agggtccgat ggacaaggat ttcaccgaga agatgcgcga atcgctgacc gagatgaaac
     3421 aggatatcct cgataagctt gtcgcgacga acgccgattt ccgggcgatc gtcgaggaga
     3481 tggatcctaa ggatttcgcc gatgtagcct ccgacgatgt cgacaggaag atgatagagg
     3541 cgctcggctc ccaggacctg aagcggctac gctcgatcga ggccgccctg gtccgtatcc
     3601 agcagggccg ctatggttcg tgcatgaagt gttcgaagcg cattccccag gaacggctcg
     3661 tggccctgcc atacgccgtg ctttgcatcg agtgccagaa gagcgaggaa cgccgcaacc
     3721 gctaggttgc gacaagcgct aggttgcgac aagcgctagg ttgcgacaag cgctaggttg
     3781 cgacaagcgc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naggtttaac
     3901 cgagctcgac ctggaagcgg ccgtctgggc ctatgacggt cagtctgtca tagcccgcgg
     3961 ccgccgccgc ccgaagcccg tcttcgcgat gcgcgctcag gtggcgggtc tcatgggcat
     4021 cggcgttcag gcatacgcct acgccgcgcg ccttcaattc tctcagtatc caggctgcgg
     4081 ggtagggcga gtcggtcttg ccccgcgcca tgccgccggt attgatctcc acgatcgccc
     4141 cgctctcggc gaccgcctcc accgccgcca tcgccgcgtc cctgtagcgg cgcccctcct
     4201 cgctgaagcg cctctggccg cggttgttct tgcggaccag gtcgaaatgc ccaagtatgt
     4261 cgaagccgcc agcgcgcgcg caggcggcca cggccgcgta gtagtcgtcg accatggctt
     4321 cggcgtcgcc gtcgtagaag cgcgagaggt ggtcgtcgaa cttttcctcc gactcgtcca
     4381 cggtgaagcc gaaggtcggc tcgccggttt cgtcgagggc ctccggggtc ggggccggcg
     4441 ctccgtccgg caggacgaaa tggaccgagc cgatgcggta gtcaaggccc gcgccgtcga
     4501 agcggccgtc ggccgggccg catagccctt cgatagaatc gacctccagc ccgacgagga
     4561 tcctcatatc ggggccgtgc gtccgcccca gcccgcgtat cgcgtcgacg tagtcgccga
     4621 ggctggcctg cttcatcgtc cacctcgtta cgaacggcag cggagcgtgg gacgagaatc
     4681 cgagtatcga gaagcccgcc cggcgcgcgg cgtccgccat agcggcggcg gtggccttgc
     4741 cgtcgcagaa ttcgcagtgg gtatggaaat tggtcagcat gggcggagta tagcgcggca
     4801 ggcctcccat aggcaacgaa gggtggcgct gcaaatatgc ttcagatatg caaagaaggg
     4861 gccttgcgcg catggatatg cagaaaatga ttgacccgga ttgtagccgg tgttactgtg
     4921 cgaggaaaca tggcgcttgg cgccatacac atccgctacg ggaggctcgc gcatgagcaa
     4981 aaaccgttca aagtgccgca taccttcgtg cttttgttcg ccttgatcat cgtcgccgtc
     5041 atcggttcgt acatcatccc ggccggtgaa ttcgatcgag ccacggatcc aaattccggg
     5101 cgtacggtcg tcgtccccgg cacgttccat cgcgtcgagc agaatcccca gcatcccttc
     5161 aagaccttca tagccctgca gaagggctcg atcgacgcgg cggacgtcgt gttcttcgtg
     5221 ttcttcgtct acgcctcgtt ctatatcgtg ttgcagaccg gggcgctgca ctcgttcatc
     5281 ggagggctcc tgagggcgct acggggcaag gaagtcgtgc tgatccccgt cttcatgtat
     5341 atcttcgcgc tcggcggctc ggtcttcggt atgttcgagg agaccttcgg cttcatcccg
     5401 ctgttcgtgg gcctggccat cgcgatgggc tacgacgcca tcgtcggcat gtgcatggtg
     5461 ttcctggccg tgggcatggg cttcgcggcg gccttcatga acccgttcac catcggcgtc
     5521 gcgcagaaag tggccgaact gccgctgttc tcgggcatgg gcttccgcat cgtcagttgg
     5581 ttcgtgttcg tgacgatggc cgtgatctgg accgtccgct acgcgctcag gatcaagaag
     5641 gacccgagca agagccacct cgccagcgtc gatatgggca agctggcgct gaaccacgat
     5701 gagctggtgg ataagaaggc taccggacgc gaccggctga tcctggcctg ggtcgtcggc
     5761 accataggcg tactcatctg gggcgtcatc gccaagggct ggtacttcga cgagctggcc
     5821 ggcctgttcg tcatcatggg cattgccacg ggcctgatcg ccgactgggg ccccagcaag
     5881 atcgccgcca ccttcctcga gggctgcaag gacatcgtct tcggcgcgct ggtcgtcggc
     5941 ttgtcgcgcg gtatcctcgt cgtcatgcgc gaggcgcaga tcatcgacac ggtcgtctac
     6001 gccatgtcgc aaccgctcgc cgcgctgccg cg
//
LOCUS       DAOPHV010000087         5995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_17333_length_5995_cov_3.028114, whole genome shotgun sequence.
ACCESSION   DAOPHV010000087 DAOPHV010000000
VERSION     DAOPHV010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17333_length_5995_cov_3.028114"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..2821)
                     /locus_tag="PLQ29_03595"
     CDS             complement(53..2821)
                     /locus_tag="PLQ29_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85761.1"
                     /translation="MKTTTGSLVIRGALALSVLFMASLAASCKSPLFGLGGQVDTAVP
                     SISVSEVEEGGTPRTLVNGDYVRGTITLRGKASDDLGLASVALSFEDNGATIRLDATI
                     DAAAQSWSATVVTTDYLDGDKDFTVTVTDTAGKASTTRFVLYFDNKPPTVLLTVPSKD
                     EGTASDALYGTIDIKGSAADQFGIRKVRVYVYDSNKTLLYTSPDDASIGTNSFALRLD
                     TSSLLGESGAEIGYIYVRAWDRSGNTNVQFYRLDELKALNANLGATVEELHALETGSA
                     TSANGITLAQAAGARRYQPGTGYADAWLIGYSFDQSSNLPIITISNPEQGKAVNLLSK
                     SAKAMGIVQDANGVSLIELQFRSGAQVSGWYSDAIPGDEGADGNLEVSGTGRVVNWSS
                     KVLYESFPDSGFGALDVASYDLRIRATDGDGNVRTSDWVPFTIDADVPYVEISDPKPG
                     DYEGGDPIVISGMAKSYGTTNVSEVAVSINGVFDYQTATILSGVGTQDVTWSFNLTSA
                     LVPTDGTYTIKARVRDSGSKEALYNIVVTIDRTPPHDLAFSSPAKGSSINGYPVLRGQ
                     AKDNILLKEVYLVLEKPGEENKLNDTYNWSYELDAGSFANTDYGYEVSTNVYRVPFRI
                     KAVDEAGNASLSPAFGADPDHPAVGSYYLDVDLDGDYPEISTIISPGDQNASDAPYVI
                     TGSIKVQGTATDDDRLHSVEMALVARSGATESWRSLASGSLYAPVAPNSFYPVTGTSL
                     WSQVLNDGGELYALSDFAGHAGDFRIIVRAVDTKDLVSVNPDPDLSGDEKTVYVRFDN
                     TLPGVINLLPEEGTMRSGTFPLTFTAQDNVRVVLAQISYNNGSNYSDLAIAPAASIPL
                     TVSVNTKTVNSNVFNTSSGTLYMRIRLKDDGNNITEKSFKYLVDNIAPVDVTPPSSTT
                     MMNISNRM"
     gene            complement(3038..3748)
                     /locus_tag="PLQ29_03600"
     CDS             complement(3038..3748)
                     /locus_tag="PLQ29_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPG85762.1"
                     /translation="MSPYGRRGYGSRPGEGRSSGRAGTGPSRRGDGAHGASRLSIDDV
                     DALFSAVGRVRFVAFDFETTGLSPERDRVVEIGAVAFRMALDESGPDGQPWRAIEDGS
                     FETLIHPGRPIPPEVSAIHGIDDLAVSGAPSFAEAAARFLPFIDGSILVAHNAPFDLG
                     FLRAETARAGLENPPNPAYDTIAIAKTAISGLPSYSLKSLASAFGIVQTVAHRGGDDA
                     KVCMNLFSRCINLIYTSK"
     gene            complement(3745..5496)
                     /locus_tag="PLQ29_03605"
     CDS             complement(3745..5496)
                     /locus_tag="PLQ29_03605"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPG85763.1"
                     /translation="MDTNTILERARDYIAKESDPSFKAEVEALIEAKDYAELEDRFYR
                     DLEFGTGGLRGVIGGGYNRMNSLVVRRATQGMCDYLNATVGGKSLSACVAYDSRRYSD
                     VFALDTALIFAANGIKAYLFTSLRPTPELSYAIRKLGADTGVVVTASHNPPAYNGYKA
                     YWNDGAQVTSPHDTGIIDRVNAVKTIKSMPRDEAIAKGLLVMIDKEIDDPYVAMVKSR
                     LFRPELIKTMAKDVKVVYTPLHGTGAMPFERVMGELGLSVVTVPEQREPNGDFPTVSY
                     PNPEESAALKMALELGAKVKADVVMATDPDADRLGIAVPDGKGGFALITGNQLGALHA
                     DYILLSLKELGRMPKRPATIKSIVTTELQARIARAYGAECHDCLTGFKWIADTMRRFE
                     RDGLDFVYATEESYGYLVEDEVRDKDGISAAAMTAEMTMYWRSKGKSLLQRLDELFVE
                     HGFHEERGVNKYFEGPTGMDVMRGIMDAYRADQPKTLGGIPVERIRDIKTGKAWSVAG
                     GPGKAETVKLPTSDVIQWYLEDGTLVTVRPSGTEPKIKYYVLARTAVGPEGLAAARKA
                     SNAKVDAILADIKKVIG"
     gene            complement(5563..>5995)
                     /locus_tag="PLQ29_03610"
     CDS             complement(5563..>5995)
                     /locus_tag="PLQ29_03610"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPG85764.1"
                     /translation="LYEAAGAGESRPYPGTIEALRSLSDAGAALAVVSNKPEPIAARV
                     IGALFPGLRFALVRGGRDGQPFKPDPTGALEAATACGVTPRECAFVGDSDVDMRTAVA
                     AGMLPVGAAWGFRGEAELRDAGAVVIAASMADVPPLLGYSY"
BASE COUNT          961 a         1769 c         2094 g         1171 t
ORIGIN      
        1 ttctcggttc aactcggaac tgaatacgct tgaatgagtt gttcaggaaa ccctacatac
       61 gattgctgat gttcatcatt gtcgttgagg aaggcggcgt tacatccacg ggcgctatgt
      121 tgtcgacgag gtacttgaaa ctcttctcgg tgatgttgtt gccgtcgtcc ttgaggcgta
      181 tgcgcatgta gagggtgccc gaggaggtgt tgaacacgtt cgagttgacg gtcttcgtgt
      241 tgacgctgac cgtcaggggg atggacgcgg ctggcgcgat cgccaggtcc gagtagttcg
      301 agccgttatt gtagctgatc tgggcgagca cgaccctgac attatcctgg gcggtgaagg
      361 tcagcgggaa ggtgccgctc cgcatggtgc cttcctcggg caggaggttg atgacgccgg
      421 gtagggtgtt atcgaagcgc acgtagacgg tcttctcgtc gcctgagaga tccgggtccg
      481 ggttgaccga tacgaggtcc ttggtgtcca ccgcgcgcac gatgatcctg aagtcgccgg
      541 cgtggcccgc gaaatcggac agggcgtaca gctcgccgcc atcgttgagc acctggctcc
      601 agagcgaggt gccggtcacc gggtagaagc tgttcggggc caccggagcg tacaggctgc
      661 cggacgctag gctccgccag ctctcggtag cgccggagcg cgcgacgagg gccatctcca
      721 ccgaatggag ccggtcgtcg tcggtggcgg tgccctgtac cttgatcgag ccggtgatca
      781 cgtacggcgc gtcggaggcg ttctggtcgc cgggggagat gatggtcgat atctccgggt
      841 agtcgccgtc caggtccacg tcgaggtagt aggagccgac cgccggatgg tccgggtcgg
      901 cgccgaaggc cgggctcagg ctcgcgttgc ccgcctcgtc gacagccttg atcctgaagg
      961 gtacacggta gacgttggtg ctcacttcgt agccgtagtc ggtatttgcg aaggagccgg
     1021 cgtcgagctc gtagctccag ttgtaggtat cgttgagctt attctcttcg ccgggtttct
     1081 cgaggacgag gtagacctct ttgaggagga tgttgtcctt ggcctgcccg cgcaatacgg
     1141 gatagccgtt gatgctcgag cccttggccg gcgaactgaa ggccaggtcg tgcggcgggg
     1201 tgcgatctat cgtgacgacg atgttgtaga gggcttcctt actgccggaa tccctgaccc
     1261 gagccttgat cgtataggta ccgtccgtcg gcacgagggc gctcgtcagg ttgaagctcc
     1321 acgtgacgtc ctgcgtgccc actccggata ggatcgtagc ggtttggtag tcgaagacgc
     1381 cgttgatcga gacggccacc tccgaaacgt tcgtggtgcc gtacgacttg gccatgccgc
     1441 ttatcacgat cggatcgccg ccctcgtagt cgccgggctt cgggtcgctt atctcgacgt
     1501 acggcacgtc cgcgtcgatg gtgaagggga cccagtcgga ggtgcgtaca ttcccgtcgc
     1561 cgtcggtggc gcggatgcga aggtcgtagg aggccacgtc gagcgcgccg aagccggagt
     1621 cggggaagct ttcataaagc actttcgagg accagttgac aacccggccc gtgccgctga
     1681 cctccagatt tccgtcagcg ccttcgtcgc cggggatcgc gtcggaatac catccggata
     1741 cttgggcccc gctcctgaac tgcaattcga tgagcgagac gccgttggcg tcctgcacga
     1801 tgcccatggc cttggcgctc ttggacagca ggttgacggc cttgccctgc tccgggttcg
     1861 agatggtgat gatcggcagg ttcgagctct ggtcgaagct gtagcctatg agccaggcgt
     1921 cggcgtagcc ggtgccgggc tggtagcggc gggcgccggc ggcctgggcc agggtgatgc
     1981 cgttggcgct ggtcgccgag ccggtctcga gggcgtggag ctcctcgacg gtggcgccga
     2041 ggttggcgtt gagggccttg agctcgtcga ggcggtagaa ctggacgttg gtgttgcccg
     2101 agcggtccca ggcgcggacg tagatatagc cgatctccgc gccgctctcg ccgagtagcg
     2161 agctggtgtc caagcgcagg gcgaaggagt tggtgccgat cgaggcgtca tcgggcgagg
     2221 tgtagagcag ggtcttgttc gagtcgtaga cgtagacgcg gaccttgcgt atgccgaact
     2281 ggtcggccgc gctacccttg atgtctatgg tgccgtagag ggcgtcggag gcggtgccct
     2341 cgtctttaga cgggacggtc aggagcacgg tcggcggctt gttgtcgaaa tacagcacga
     2401 agcgggtggt ggaggccttg ccggcggtgt cggtaacggt gacggtgaag tccttgtcgc
     2461 cgtcgaggta gtcggtggtg acgacggtgg cgctccagct ctgggccgcg gcgtctatcg
     2521 tggcgtccag cctgatcgtg gcgccgttat cctcgaacga tagcgctacg gaggctaggc
     2581 cgaggtcgtc ggaggctttt ccgcgtaggg ttatggtgcc gcggacgtag tctccgttga
     2641 cgagggttcg cggggttccg ccttcctcga cctcggagac tgagatgctc ggtaccgcgg
     2701 tgtcgacttg gcctccgagc ccgaagaggg ggctcttgca ggaagcggcc agggaggcca
     2761 tgaaaagcac agaaagcgct agcgcgccgc ggattacgag cgacccagtc gtcgttttca
     2821 taccaactcc ctacacggac ggtatcggcg cgcatgcgcc cgattctact atcggcacaa
     2881 tgccggcagg cgaaaacttg ggtcttctat ttaatacaat atcacacagc gacctatttg
     2941 tcgatatcga caaatatgta ccatgataaa gtgtagtttg aaggatggag aaaacaattc
     3001 gacaaatacg tcgatgcgac gaaattgtcg aattgtttta tttgcttgtg tagattaggt
     3061 ttatgcagcg ggaaaacaaa ttcatgcaga cttttgcgtc gtctccaccg cggtgggcta
     3121 ccgtctgcac gatgccgaac gccgaagcga gcgacttgag cgaatagctg ggcaggcccg
     3181 agatggccgt cttcgctata gctatagtat catacgccgg attcggcggg ttttcaagcc
     3241 cggcgcgggc ggtttccgcc ctgaggaagc cgaggtcgaa cggggcgtta tgggcgacga
     3301 gtatcgagcc gtcgatgaag ggcaggaagc gggcggcggc ctcggcgaag ctcggggccc
     3361 cggagacggc caggtcgtcg atgccgtgga tggcgctgac ttccggaggt atcggccggc
     3421 ccggatggat cagggtctcg aacgagccgt cctcgatcgc gcgccacggc tggccgtccg
     3481 gtccggactc gtccagggcc atccggaacg cgacggcgcc tatctcgacg acgcggtcgc
     3541 gctctgggga caggcccgtc gtctcgaagt cgaacgcgac gaagcgaacg cgcccgacgg
     3601 cggagaacag ggcgtcgacg tcgtcgatcg aaagcctcga ggcgccatgg gcgccatcgc
     3661 cgcgcctcga cgggccggtc cccgcccggc ctgaggatcg cccctcgccc ggacgggagc
     3721 cgtagccgcg acggccgtag gggctcaacc gatcactttc ttgatgtcgg ccaggatagc
     3781 gtcgaccttg gcgttcgaag ccttcctggc ggcggcgagc ccctccgggc ccaccgcggt
     3841 gcgggcgagc acgtagtact tgatcttagg ctcggtgccg ctcgggcgca cggtgacgag
     3901 ggtgccgtcc tccaggtacc actggatcac gtcgctcgtc ggcagcttta ccgtctccgc
     3961 cttgccgggg ccgcctgcga cgctccaggc cttgccggtc ttgatatcgc ggatgcgctc
     4021 gaccggtatg ccgccgaggg tcttgggctg gtcggcgcgg taggcgtcca tgatgccgcg
     4081 catcacgtcc atgccggtcg gcccctcgaa atacttgttg acgccgcgct cctcgtggaa
     4141 gccgtgctcg acgaacagct cgtccaggcg ctggaggagg ctcttgccct tggagcgcca
     4201 gtacatggtc atttcggcgg tcatcgcggc ggccgagatg ccgtccttgt cgcggacctc
     4261 gtcctcgacg aggtagccgt agctctcctc ggtggcgtag acgaaatcga gcccgtcgcg
     4321 ctcgaagcgg cgcatcgtgt cggctatcca cttgaaaccg gtcaggcagt cgtggcactc
     4381 ggcgccgtag gcgcgggcta tgcgcgcctg cagttcggtg gtgacgatgc tctttatcgt
     4441 cgccggtcgc ttgggcatgc ggccgagctc cttcagggac aggaggatat agtcggcgtg
     4501 gagggcgccg agctggttgc ccgtgatcag ggcgaagccg cctttgccgt ctggcacagc
     4561 gatgccgagc cggtcggcgt cggggtcggt ggccatgacg acgtcggcct tgactttcgc
     4621 gccaagttcc agcgccatct tgagagcggc cgactcctcc gggttcgggt aggagacagt
     4681 cgggaagtcc ccgttcggct cgcgttgctc cggcacggtg acgaccgaca gaccgagctc
     4741 gcccatgacg cgctcgaagg gcatggcccc ggtgccatgg agcggggtgt agaccacctt
     4801 gacgtccttg gccatggttt tgatcagctc gggcctgaat aacctagact tgaccatggc
     4861 gacgtacggg tcgtcgatct ccttgtcgat catgacgagg aggcccttgg cgatggcctc
     4921 gtccctgggc atcgacttga tcgtcttcac ggcgttcacc cggtcgatga tgcccgtgtc
     4981 gtggggggag gtgacctggg cgccgtcgtt ccagtaggcc ttgtagccgt tgtaggccgg
     5041 ggggttgtgg ctcgcggtga cgaccacgcc cgtgtcggcg ccgagcttcc tgatcgcgta
     5101 ggacagctcg ggggtcggcc gtagcgaggt gaacaggtag gccttgatgc cgttggccgc
     5161 gaagatcaga gccgtgtcga gcgcgaacac gtccgagtag cggcgcgagt cgtaggcgac
     5221 gcaggcggac aggctcttgc cgccaacggt cgcgttcagg tagtcgcaca tgccctgggt
     5281 cgcgcggcgg accacgagcg agttcatgcg gttgtagccg ccgccgatca cgccgcgaag
     5341 gccaccggtg ccgaattcta ggtcgcggta gaagcggtcc tcgagctccg catagtcttt
     5401 ggcctcgatc agggcctcga cctcggcctt gaagctcggg tcgctctcct tggcgatata
     5461 gtcgcgcgct cgttccaata tggtattcgt atccatcatc gttccccttt cggtcggtcg
     5521 ttccaaatat agcttccggc ggtagctaag tgaagcctgg cgctagtaag aatatcccag
     5581 gaggggcggc acgtcggcca tcgaggcggc gatcacgacg gccccggcgt cgcgtagctc
     5641 cgcctcgccg cggaagcccc aggccgcccc gacgggcagc atgccggccg ccacggccgt
     5701 cctcatgtcc acgtccgagt cgccgacgaa ggcgcactcg cggggggtaa cgccgcaggc
     5761 cgtcgcggcc tcgagcgctc ccgtcgggtc cggcttgaac ggctgaccgt cgcggccgcc
     5821 gcgtacgagc gcgaagcgaa ggccggggaa cagggcgccg atcacgcgcg ccgcgatggg
     5881 ctctggctta ttcgagacga cggccagggc cgctccggca tcggacagcg accgcagcgc
     5941 ctcgatggtc ccggggtagg gacgggattc tccggcgccg gccgcctcgt acagg
//
LOCUS       DAOPHV010000088         5951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_17523_length_5951_cov_3.525271, whole genome shotgun sequence.
ACCESSION   DAOPHV010000088 DAOPHV010000000
VERSION     DAOPHV010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17523_length_5951_cov_3.525271"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLQ29_03615"
     CDS             <1..205
                     /locus_tag="PLQ29_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85765.1"
                     /translation="AVFDVLSATDAKTFSELGGDWLKSSIAVAKSLRGLDEATRKVMF
                     DALKLLFKAIRENLPAIMQKRPQ"
     gene            302..1570
                     /locus_tag="PLQ29_03620"
     CDS             302..1570
                     /locus_tag="PLQ29_03620"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPG85766.1"
                     /translation="MRRRPVPLIVPTSAVIAGSYVLALLFFSLAVGASSNHPWFAGTY
                     YGCVAASAALSVLSVALGQAWVSGFIACLRCYLIIVLGYGVSAYLSVKLTLGAGLMVE
                     VCALVPYPANLALCAALGSAIVLAQVFPGFMGASQLVEATARARPDEVLALAALLAMA
                     AGAAALIGRLVSVNDSLGRALRMQERSVDTLAELNKNLQGYARTADEDSAERERARIS
                     REIHDISGYIFTNLIALMDAAGSLPPDDRETLLDLVGTARKQAQEGLQETRVALRKLR
                     DEKPRMADNARAMHKIVSIFRTVAGIDVDLELGNLPRLLPTELNLTLYRAIQEGLTNA
                     VRHGKATAVRIRFWVDDDLVRLSIADNGRGADELTKGIGLSGMEERVGSLGGSVRTGR
                     SPEGGFLLSVDVPLGTGPASTPARKAEATA"
     gene            1567..2259
                     /locus_tag="PLQ29_03625"
     CDS             1567..2259
                     /locus_tag="PLQ29_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPG85767.1"
                     /translation="MIRVLIADDQRLFGESLSTTLRNYAKDIDVVGLASDGAEAIELV
                     GRLSPDIVLMDVFMPRLDGVEATRSIMARWPAVRILMLSTYDEDTKVKEALSLGAAGY
                     LLKDITPVELIASIRALSSGVMQISPQIVARLMQGALAEGDVPDRRAQGGRSAASRLA
                     DSLTGLDALTSREREVLALIADGFDNTHIASELCLAEHTVRNHVSAIYAKLEVQDRFQ
                     IIQMAKAARSDR"
     gene            2384..3691
                     /locus_tag="PLQ29_03630"
     CDS             2384..3691
                     /locus_tag="PLQ29_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPG85768.1"
                     /translation="MKRTRFAAIAMIAIMALTLGALSSCGGQPKMTDTVTVWCWDPNF
                     NLYAMKEAVAVYQKANPGVKVEIVDMAQDIEAKIISGLQAGGAGLPDIALFQDFRIEQ
                     FLSDYPDAFVDLKAEGVDYSQFAPYKVGPMTVGGKVYGIPFDTGSTGLWLRTDYMKEA
                     GIDPDAYLGKNLTWSEVIALGEKIKAKTGKPLLAYQSDNFDMLRIVVQSTGGQFFKDD
                     GSLNLRSEAFKKSLALFKELNDKGLLYQVVGWSDWINAINSDTSAGFLNAIWMVGSVK
                     SRPENAGKYMVLPTPLIEGVPGAANASNNGGSSWYVFLSSKNKARAVDLLKSVWATAT
                     PDNLEFYNKILKDAGAMGTFLPSQSGSNYKAGDDFFYKGQALSKDFASWMANVPVLKY
                     TPNYVPMRDAVAHATLNYFQGKLATIDEAIAAAETEYKQVTGK"
     gene            3779..4672
                     /locus_tag="PLQ29_03635"
     CDS             3779..4672
                     /locus_tag="PLQ29_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPG85769.1"
                     /translation="MKQKASFQRYLNRFGWFCVLPSVILFGVFMLYPILSSLYYVTRR
                     WKGMSNTFIGLGNFIRMAQDKVFLSALGNNFLFMVVQIPLMVFLALVLAVILNQGIRR
                     WKGAFRLAIFLPAVTSLVVVSVLFRVLLQENGMLNNVLLKAHLITQPIGWLNDAAWAK
                     VTIILAMTWRWTGYNMVFFLVGLQSIDPQIYEAAEVDGASKTRQFFTITIPLLIPVIL
                     FSVVTSTSGTMQLFDEPNILTWQGGPANATMTTALYIYRQAFFINADFGYATAMSYVV
                     VLISGIFAFAQMRLLGDRGNS"
     gene            4669..5490
                     /locus_tag="PLQ29_03640"
     CDS             4669..5490
                     /locus_tag="PLQ29_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPG85770.1"
                     /translation="MSMVRSWTLGRVISLVVLLVATAFSVFPFVWMLVGATLNPNEVL
                     RGALRPGAEFVSNWVKATRMYNLPLFFWNSLKIAVVTVLLGLLVNGFAAYGFEKYPSK
                     ARDRVFNVLLLTLIIPQIALVIPMFRMFAFLRLLDSHAAIILPAIMSVFIIFFMRQNF
                     KMFPSEIIEAARADGASELTIFVRIVTPSMQATYVSSAIYIFINQWNSYLWPLITILS
                     DAKKTMPIAISSMMSAYTIEYGALMVIVCISVLPVLVIFLALQRQFVAGLLGSVK"
     gene            5504..>5951
                     /locus_tag="PLQ29_03645"
     CDS             5504..>5951
                     /locus_tag="PLQ29_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HPG85771.1"
                     /translation="MNRHQFFSTRPWESPETQGLGRLPMRAPLLPWPSAGRALAAAAL
                     GPTSGGNDNPLVLSLDGRWRFSLAANPEATPEGFHEPGFDDRAWPYLNVPGTWTLQGF
                     DKPHYTNVVMPFGNVPPAPPAADNPTGLYRVGFELPPSWEGRRVVLH"
BASE COUNT         1052 a         2069 c         1793 g         1037 t
ORIGIN      
        1 cgccgtcttc gacgtcctgt cggcgacgga cgccaagacc ttctccgagc tgggcggcga
       61 ttggctcaaa agttccatag ccgtggcgaa gtcccttcgc ggcctcgacg aggcgaccag
      121 gaaggtcatg ttcgacgcgc tcaagcttct atttaaagct atccgggaaa accttccggc
      181 catcatgcag aaacggcctc agtagcatgg acacgtcgtc gtcgtcgacc gatgctcgcg
      241 ctatgcgact accaggttcc cccgccgaac gccacgagcg gatggcggga taggctcgtc
      301 tatgcgccgc cggcccgttc cgctcatcgt cccgacctcc gccgtcatcg ccggtagtta
      361 cgtcctggcc ctgctgttct tttccctcgc ggtcggagcc agctcgaatc atccgtggtt
      421 cgccggcacc tactacggct gcgtcgcggc atcagcggcg ctcagcgtcc tgtccgtcgc
      481 cctcgggcag gcctgggtct cgggcttcat agcctgcctg cgctgctacc tgatcatcgt
      541 gctcggctac ggcgtctcgg cctacctgtc ggtcaagctg acgctcggag ccgggctgat
      601 ggtcgaggtc tgcgctctcg tgccctaccc ggccaacctg gccctctgcg cggcgctcgg
      661 ctcggccatc gtactggcgc aagtcttccc gggcttcatg ggcgcgagcc agctggtcga
      721 ggccacggcc agggcccgcc cggacgaggt cctggctctc gctgcgctcc tggccatggc
      781 cgccggggcg gctgccctga tcggccgtct cgtctccgtc aatgactcgc tcgggcgagc
      841 cttgcgcatg caggaacgct cggtcgacac cctggccgaa ctcaacaaga acctgcaggg
      901 ctacgcccgc actgcggacg aggattcggc cgagcgagag cgagcccgca tcagccgcga
      961 gatccacgat atctctggat atatattcac gaacctcatc gccctgatgg acgcggcggg
     1021 gagcctgccg ccggacgacc gggaaaccct gctcgacctc gtcggtacgg ccaggaaaca
     1081 ggcgcaagag gggctccagg agacgagggt cgccctgcgc aagctacgcg acgagaagcc
     1141 gcgcatggcc gacaacgccc gggcgatgca caagatcgtc tcgatcttca ggacggtggc
     1201 gggcatcgac gtcgacctcg agctcggcaa cctgccgagg ctgctcccga ccgaactcaa
     1261 cctgacgctg tatcgcgcca tccaggaggg gctgaccaac gcggtacgcc acggcaaggc
     1321 cacggcggta cgcatacgct tctgggtcga cgacgacctc gtccgcctct cgatagccga
     1381 taatggccgc ggggccgatg agcttaccaa gggcataggc ctatcgggca tggaggagcg
     1441 ggtcggctcg ctcggcggct cggtacgcac ggggcggtcg ccggagggag gcttcctgct
     1501 ctccgtcgac gtaccgctcg ggaccgggcc cgcctcgacg ccggccagaa aggcggaggc
     1561 gaccgcgtga tacgggtact catcgctgac gaccagcgcc tgttcgggga gagcctgtcg
     1621 acgacgctca ggaactacgc caaggacatc gacgtggtgg ggctagcctc cgacggggcc
     1681 gaggcgatcg agctggtcgg gcgcctatcg ccggacatcg tgttgatgga cgtcttcatg
     1741 cccaggctcg acggggtcga ggcgacgagg agcatcatgg cgcgctggcc ggccgtccgc
     1801 atcctgatgc tatccaccta cgacgaggat acgaaagtca aggaagccct gtcgctcggg
     1861 gccgccgggt acctcctcaa ggacatcacg ccggtagagc tcatagcgag catccgggcc
     1921 ctgtcgagcg gcgtcatgca gatctcgccg cagatcgtcg ccaggctcat gcagggggcg
     1981 ctcgcagagg gcgacgtgcc tgatcggaga gcccagggcg gccgtagcgc cgccagtcgc
     2041 ctagccgaca gtcttaccgg gctcgacgcc ctgacctcgc gcgagcgaga ggtgctcgcg
     2101 ctgatagccg acggcttcga caacacccat atcgcctccg agctgtgcct cgccgagcat
     2161 acggtgcgca accacgtgag cgccatctac gccaagctcg aggtccagga ccgcttccag
     2221 atcatccaga tggccaaggc ggcccgttcc gacagataga ctcgggacaa ttgtaccgcc
     2281 cgcggtctcc aaaaccgtga caactgtctc ttgcggattc cggttccggc ctcattatgg
     2341 acgcaagcgg cgcacggcgc cggattccca taggaggcta cacgtgaaac gaacccgatt
     2401 cgcggcgatc gccatgatcg ccatcatggc cctgaccctc ggcgccctct cgtcctgcgg
     2461 cgggcagccg aagatgaccg acaccgttac cgtctggtgc tgggacccta acttcaacct
     2521 gtacgccatg aaggaagcgg tcgccgtcta ccagaaggcc aacccgggcg tcaaggtcga
     2581 gatcgtcgac atggcccagg acatcgaggc caagatcatc tccggcctcc aggccggcgg
     2641 cgccggcctc ccggacatcg cgctgttcca ggacttccgc atcgagcagt tcctgtcaga
     2701 ctacccggac gccttcgtcg atctcaaggc cgagggcgtc gactatagcc agttcgcccc
     2761 gtataaggtc ggcccgatga ccgtgggcgg caaggtctac ggcatcccct tcgacaccgg
     2821 ctcgaccggc ctctggctgc gcaccgacta catgaaggag gccggcatcg atcccgacgc
     2881 ctacctcggc aagaacctca cctggtccga ggtgatagcc ctcggcgaga agatcaaggc
     2941 caagaccggc aagcccctgc tcgcctacca gagcgacaac ttcgacatgc tgcgtatcgt
     3001 cgtccagtcg accggcggcc agttcttcaa ggacgacggc agcctgaacc tccgcagcga
     3061 ggccttcaag aagtcgctcg ccctgttcaa ggaactgaac gacaagggcc tgctctatca
     3121 ggtggtcggc tggtccgact ggatcaacgc catcaacagc gacacgagcg ccggtttcct
     3181 gaacgccatc tggatggtcg gtagcgtcaa gtcgcgcccc gagaacgcgg gcaagtacat
     3241 ggtcctcccg acgccgctca tcgagggcgt gcccggagcg gccaacgctt cgaataacgg
     3301 cggctcgagc tggtacgtct tcttgtccag caagaacaag gcccgcgcgg tcgacctgct
     3361 caagtccgtc tgggctaccg ccaccccgga caacctcgag ttctacaaca agatactcaa
     3421 ggacgccggc gccatgggca ccttcctgcc gagccagtcc ggctccaact acaaggccgg
     3481 cgacgatttc ttctataaag gccaggcgct ctccaaggac ttcgcctcgt ggatggcgaa
     3541 cgtgccggtt ctcaagtaca cgccgaacta cgtgccgatg cgcgacgccg tggcccacgc
     3601 cacgctcaac tacttccagg gcaagctagc gacgatcgac gaggccatag cggcggccga
     3661 gaccgagtac aagcaggtga ccggcaaata acggacgccc gataaggagc cgcgccgacg
     3721 ggccttccgc cggcggggtt cccccgcgtt cccccagtcc aggcaaccag gagcccccat
     3781 gaaacagaag gccagctttc aacgttacct gaaccgcttc ggctggttct gcgtcctgcc
     3841 gtcggtcata ctcttcggcg tcttcatgct ctaccccatc ctgtcgtcgc tgtactacgt
     3901 gacgcgccgc tggaagggca tgagcaacac cttcatcggg ctcggcaact ttatccgcat
     3961 ggcccaggac aaggtcttcc tgtccgccct cggcaacaac ttcctgttca tggtcgtaca
     4021 gataccgctg atggtgttcc tggcgctcgt actggccgtg atactcaacc agggcatccg
     4081 gcgctggaag ggagccttca ggctggccat cttcctgccg gccgtcacct cgctggtcgt
     4141 cgtgtcggtg ctgttcaggg tactcctcca ggagaacggc atgctcaaca acgtgcttct
     4201 caaggcgcac ctgataaccc agccgatagg ctggctcaac gacgcggcct gggccaaggt
     4261 caccatcatc ctcgcgatga cctggcgctg gaccggctac aacatggtct tcttcctcgt
     4321 cgggctccag agcatcgacc cgcagatcta cgaggcggca gaggtcgacg gagcgtcgaa
     4381 aacgaggcag ttcttcacga taacgatacc gctcctcata ccggtgatcc tgttctccgt
     4441 cgtcacctcg acgagcggca cgatgcagct cttcgacgag ccgaatatcc tgacctggca
     4501 gggagggccg gccaacgcga cgatgaccac ggccctgtac atctaccggc aggccttctt
     4561 catcaacgcg gatttcggct acgccaccgc gatgtcgtac gtcgtcgtgc tcatctcggg
     4621 catattcgcc ttcgcgcaga tgcgcctgct cggcgacagg gggaactcat gagcatggta
     4681 cgctcctgga cgctcggacg ggtcatcagc ctcgtcgtgc tgctcgtcgc cacggccttc
     4741 agcgtcttcc ccttcgtatg gatgctcgtc ggggccacgc tcaacccgaa cgaggtgctg
     4801 cgcggcgcgc tccggccggg cgcggagttc gtctcgaact gggtcaaggc cacgcgcatg
     4861 tacaacctgc cgctgttctt ctggaactcg ctcaagatag ccgtcgtcac cgtgctactc
     4921 ggcctgctcg tcaacggctt cgcggcctac ggcttcgaga aatacccgtc caaggcgcgc
     4981 gaccgggtct tcaacgtgct gctcctgacg ctcatcatcc cgcagatagc cctcgtcatc
     5041 ccgatgttca ggatgttcgc cttcctccgg ctgctcgact cgcacgccgc catcatcctg
     5101 ccggccatca tgtcggtgtt catcatcttc ttcatgcgcc agaacttcaa gatgttcccg
     5161 tccgagatca tcgaggcggc gcgggccgac ggggcgagcg agctgaccat cttcgtccgc
     5221 atcgtcacgc cgtcgatgca ggcgacctac gtatcgtcgg ccatctacat cttcatcaac
     5281 cagtggaact cgtacctctg gccgctcatc accatactga gcgacgccaa gaagacgatg
     5341 cccatcgcga tcagctcgat gatgagcgcc tatacgatag agtacggagc cctgatggtc
     5401 atcgtctgca tctcggtcct gccggtgctc gtcatcttcc tggccctgca gcgtcagttc
     5461 gtcgccgggc tgctcggatc ggtcaagtaa cgcggaggct cgcatgaacc ggcaccaatt
     5521 cttctcgacg cggccctggg aatccccgga gacgcagggc ctcgggcgcc tgccgatgcg
     5581 cgcccccctc ctgccctggc cgagcgcggg gcgggccctc gctgcggccg ccctcgggcc
     5641 gacctccggc gggaacgaca atccgctcgt cctgagcctc gacgggcgct ggcgcttcag
     5701 cctcgccgcc aaccccgaag cgacgcccga aggcttccac gaaccaggct tcgacgaccg
     5761 ggcgtggccc tacctgaacg tgccgggcac ctggacgctc cagggcttcg acaagcctca
     5821 ctacaccaac gtggtcatgc ccttcggcaa cgtaccgccg gcgcctccgg cggcggacaa
     5881 cccgaccggc ctctaccgcg tcggcttcga gctgcccccg tcgtgggaag gccggcgcgt
     5941 cgtcctccac g
//
LOCUS       DAOPHV010000089         5915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_17674_length_5915_cov_4.725256, whole genome shotgun sequence.
ACCESSION   DAOPHV010000089 DAOPHV010000000
VERSION     DAOPHV010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17674_length_5915_cov_4.725256"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="PLQ29_03650"
     CDS             <1..1181
                     /locus_tag="PLQ29_03650"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG85772.1"
                     /translation="AAPIASVAFGAWGGHLADRWGRKPVQILGVSLSASALFGFAFAG
                     SNVYVLALLNFLNGMTRTFYRPATNAAIADFAPPERRSEAYALHRIAINAAFGWGPLI
                     GVGLFLVAPRTGFIVSGVLNLLVGVFIALAIPESSSPSESASPLKSASPLKSASPLKS
                     ASPSESSSPSESAQVGERLDRLGAATRPAWRAIAADGVFWLWTFGMMLVWGAYDLIQS
                     FLPLHLTEAGVPLWVYGSALTVNALVCVFAQLPASRLLRVAPIGPSAGASKLAFAVGF
                     LGLAFLRSPAWLLAAMLVLSIGEVWGSAVQSRFVPEHASPELLGRYLGLSTLFELGKA
                     FVAPAAGFLMQAAGGSAVFAMAAVLSVAGGSLLYLAGSRHDGSGLVPARSARSTRARA
                     "
     gene            complement(1191..2237)
                     /locus_tag="PLQ29_03655"
     CDS             complement(1191..2237)
                     /locus_tag="PLQ29_03655"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPG85773.1"
                     /translation="MMKDIRRALMGAVLLCCSVGPLVAQTALPGRPDPVASLPDGLYA
                     VIETVRGTVVLELYADKAPFAVASFVGLAEGSFSQDGRPYYDGVSFHRVEPGFVVQGG
                     DPTGTGRGGPGYQFPNEITPELGFNAPGVLGMANAGPDTNGSQFFITLGAANHLNGGY
                     TVFGRVVVGMDAVRSIRKGDAMTRVTVARKGAKARAFKADKARFDADLRAYASGAAER
                     AKAGVEGQFAAVKARIPGLALGADGLWYKALAAGNGAKPATGSMVRILYTLTLADGRR
                     LDSTADRGNEPLEFRLGGGQVIPGFDKAVSAMSYGERRVVVIPPELGYGSRGAGGVVP
                     PDAVLVFEIELLKP"
     gene            2311..4767
                     /locus_tag="PLQ29_03660"
     CDS             2311..4767
                     /locus_tag="PLQ29_03660"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPG85774.1"
                     /translation="MKTDTVEIKGMSCSACARASERAAAGVPGVSNASVNFATEALTV
                     EYDEGATGLAAIAAAIAEAGYEAVLPTAVKSASVPIGGMSCAACAAAVERAVSLVPGV
                     SSASVNFASERLSVSWDPSVARLSSIKLAVKEAGYEALAADSGPAAVDEHGMAKAREA
                     RWQKRRFVVALATALPLLYVSMGHMIGLPLPPFLHPMHEPLNFALAQAALTLPALWAG
                     RKFYTVGVKALLRRAPNMDSLIAIGTGSAMAYSLWSTIQIALGDASAAGHLYFETVAV
                     IIALILLGKYLEARSKGKASEAIKRLMGLAPKTAVVVSESGDLELPVDEVAVGDVIRV
                     RPGERVPVDGVVVEGSTSVDESMLTGESLPVEKSPGDKVSGATVNGQAMFTFRASAVG
                     ADTALARIVRLVEEAQGSKAPIAALADRVSGVFVPVVVAIAFASAVAWLIGGQSFEFA
                     MRVFVAVLTIACPCALGLATPVAIMVGTGRGAELGLLIKGGEALENAHRVTAVALDKT
                     GTVTLGKPAVVEVLAYGHLDEEAALALAASAERGSEHPLGAAIVREAEARSLALSSPD
                     SLKALPGRGIEARLGGAEAIVGNARLMEERGIDVGPAAADLERLAADGKTAMLVAAEG
                     RLVGLVAVADIVRPTSRAAIAALRARGVETTMITGDSRAVAEAIGREVGVDRVVAEVL
                     PEDKSAAVRALQASGKLVAMVGDGINDAPALAAADVGIAIGSGTDIAAESADIVLARG
                     DLEDAVAALDLSRATMRVIRQNLFWAFGYNVLGIPVAAGLLHLFGGPLLNPMIAAAAM
                     SLSSVSVVTNALRLKTWKPI"
     gene            4791..5102
                     /locus_tag="PLQ29_03665"
     CDS             4791..5102
                     /locus_tag="PLQ29_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HPG85775.1"
                     /translation="MDTCDNQADGCSCKKTERPEELKRSLDGRLARIEGQVRGLRRMI
                     EGDAYCDDVMTQIAAARGALGKASLVVLEHHLKHCLIDHVRAGDDDIVDELLDSLAKN
                     F"
     gene            5129..5476
                     /locus_tag="PLQ29_03670"
     CDS             5129..5476
                     /locus_tag="PLQ29_03670"
                     /inference="COORDINATES: protein motif:HMM:NF021498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2220 domain-containing protein"
                     /protein_id="HPG85776.1"
                     /translation="MPQPGASVILGGGYGFSQLADAAWLRGPRLRYWGDLDTHGFAIL
                     DQFRSAFPEAESFLMDRITLLEHREPWTTEPTPTRAELSRLSPEEAGLYDDLRYDSLS
                     ASLRLEQEWLRFS"
     gene            complement(5522..>5915)
                     /locus_tag="PLQ29_03675"
     CDS             complement(5522..>5915)
                     /locus_tag="PLQ29_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HPG85777.1"
                     /translation="EAGFGAVLAAWVPALSRSVRLALVLASAAWLSGGMSPVELRDAL
                     ITILRPLGNRLGGRVARSASLTMAFLPWTAEEARAADEAARLRGSDPSRRPVRHVVAL
                     SVPLVSRSLEKARRSSEALSLRDPRFGE"
BASE COUNT          873 a         2099 c         2026 g          917 t
ORIGIN      
        1 gcgcggctcc gatcgccagt gtcgccttcg gcgcctgggg cgggcacctg gccgaccgat
       61 ggggccgcaa gccggtgcag atactcggcg tatccttgag cgcctcggcc ttgttcggct
      121 tcgcgttcgc cgggtcgaac gtctacgtac tcgcgctact caacttcttg aacggcatga
      181 cgcgcacctt ctatcgtccg gcgaccaacg ccgcgattgc cgacttcgcg ccgcccgagc
      241 gccgatcgga agcctacgcg ctgcaccgca tcgccatcaa cgccgccttc ggctggggcc
      301 cgctgatcgg cgtcgggctg ttcctcgtcg cgccgcggac cggcttcatc gtcagcggcg
      361 tcctgaacct gctcgtcggc gtgttcatag cgctcgccat acccgagtca tcgtctcctt
      421 cggagtcggc gtctcctctg aagtcggcgt ctcctctgaa gtcggcgtct cctctgaagt
      481 cggcgtctcc ttcggagtca tcgtctcctt cggagtcggc gcaggtcggc gagcggctcg
      541 accgcttggg cgccgcgacg aggccagcct ggcgagccat cgcggccgat ggcgtcttct
      601 ggctctggac cttcgggatg atgctcgtct ggggcgccta cgacctgatc cagagcttct
      661 tgccgctcca cctgaccgag gcaggcgtgc cgctctgggt ctacggctcg gcgctgacgg
      721 tcaacgccct cgtctgcgta ttcgcgcagc tgcccgcgag ccgccttctt cgcgtagcgc
      781 cgatcgggcc gtcggcgggc gcgtcgaagc tggctttcgc cgtcggattt ctaggcctgg
      841 ccttcctgcg ctcgccggcc tggctactcg cggcgatgct cgtcctgtcc atcggcgagg
      901 tctggggctc cgcggtgcag tcgcgcttcg tgccggagca cgcgtcgccg gagcttctcg
      961 gccgctacct gggcctaagc acgctgttcg agctcggcaa ggccttcgtg gcgccggccg
     1021 ccggcttcct gatgcaggcc gccggcggct cggccgtgtt cgccatggcg gccgtcttgt
     1081 cggtcgccgg cggctcgctc ctctacctgg ccggctcgcg ccacgacggg agcgggctcg
     1141 tcccggcccg atcggcccgg tcgactcggg cgcgggcctg agccgcgggc tcagggcttc
     1201 agcagctcga tctcgaacac cagcacggcg tccggcggca cgaccccgcc agccccgcgc
     1261 gagccgtaac ccagctccgg cggtatgacg acgacgcggc gctcgccgta gctcatagcg
     1321 gacaccgcct tgtcgaagcc gggtatcacc tggccgccgc ccagcctgaa ctcgagcggc
     1381 tcgttgccgc ggtcggccgt cgagtcgagc cggcggccgt cagccagcgt gagggtatac
     1441 aggatgcgca ccatcgagcc ggtggccggc ttggcgccgt tcccggcggc gagcgccttg
     1501 taccacagcc cgtcggcgcc gagcgccagg ccgggtatcc gcgccttgac cgcagcgaac
     1561 tgcccctcga cgccagcctt ggcccgttcg gcggccccgg acgcgtaggc gcgcaggtcg
     1621 gcgtcgaagc gggccttgtc cgccttgaac gcccgggctt tggctccctt gcgcgcgacg
     1681 gtcaccctgg tcatcgcgtc gcccttgcgg atggagcgga ccgcgtccat accgacgacc
     1741 acgcgcccga agaccgtgta cccgccgttc aggtggttcg cggcgccgag cgttatgaag
     1801 aactggctac cgttcgtatc cggccccgcg ttggccatgc cgagcacgcc cggggcgttg
     1861 aagccgagct ccggggttat ctcgttcggg aactggtagc ccgggccgcc gcggccggta
     1921 cccgtcgggt cgccgccctg gaccacgaag cccggctcca cgcgatggaa gctgaccccg
     1981 tcgtagtacg gccgcccgtc ctgcgagaac gagccctcgg cgaggccgac gaagctggcg
     2041 accgcgaagg gcgccttgtc ggcgtacagc tcgagtacga cggtaccgcg gaccgtctcg
     2101 atcacggcgt acagcccgtc cggcaacgag gcgaccggat ccggccgacc tggcagggcc
     2161 gtttgggcga cgagcggccc gacggaacag cacaatagaa ccgcgcccat caaggcccga
     2221 cggatatctt tcatcatgat cctccgcggc gaggatacgc tatggacgat cgaacggcaa
     2281 gttggtatta tacccccaag gggtatatcg atgaagaccg acaccgtcga gatcaagggg
     2341 atgagctgct ccgcctgcgc cagggcgagc gaacgggccg ccgccggcgt accgggcgtc
     2401 tcgaacgctt cggtcaactt cgccaccgag gcgctaaccg tcgaatacga cgagggcgcg
     2461 acagggctgg cggccatcgc cgcggcgata gcggaggccg gctacgaagc cgtgctaccg
     2521 acggcggtca agagcgcgtc cgtgccgata ggcggcatga gctgcgccgc ctgcgccgcg
     2581 gccgtcgaga gggcggtgtc actggtaccc ggcgtctcga gcgcctcggt gaatttcgcc
     2641 tccgagcggc tgtcggtgtc ctgggacccg tcggtcgccc gcctgtcgtc catcaagctg
     2701 gccgtgaagg aggcgggcta cgaggcgctg gcggccgact caggcccggc cgccgtcgac
     2761 gagcacggca tggccaaggc gcgcgaagcg cgttggcaaa aacggcgttt cgtcgtcgcg
     2821 ctagcgacgg cccttccact cctctacgta tcgatgggcc acatgatcgg attgccgcta
     2881 ccgcccttcc tgcatcccat gcacgagccc ctgaacttcg cgctggccca ggcggcgctg
     2941 accctgccgg cgctatgggc cgggaggaaa ttctacaccg tcggcgtcaa ggccctgctc
     3001 aggcgagcgc ctaatatgga cagcctgatc gcgataggca ccggctcggc gatggcctac
     3061 agcctctggt cgaccataca gatcgccctc ggagacgcct ccgcggccgg acacctgtac
     3121 ttcgagaccg tcgccgtcat catcgccctg atcctgctcg gcaagtacct cgaggcccgg
     3181 tccaagggca aggcatcgga ggccatcaag cggctaatgg gcctggcgcc caagaccgca
     3241 gtggtcgtgt cagaatcggg cgacctggag ctgccggtcg acgaggtggc cgtcggcgac
     3301 gtgatccgcg tgcgccccgg cgaacgagtg ccggtcgacg gcgtcgtcgt cgaaggctcg
     3361 accagcgtcg acgagtccat gttgaccgga gagagcctgc ccgtcgagaa gagcccgggc
     3421 gacaaggtat ccggcgccac cgtcaacggc caggccatgt tcaccttcag ggcgagcgcc
     3481 gtcggcgccg acacggccct ggcccgcatc gtccgcctag tcgaggaggc ccagggctcc
     3541 aaggccccga tagccgcgct cgccgaccgg gtatcgggcg tcttcgtacc ggtcgtcgtc
     3601 gccatagcct tcgcctccgc ggtcgcgtgg ctgatcgggg gacagagctt cgagttcgcg
     3661 atgcgggtgt tcgtggccgt actgacgata gcctgcccct gcgcgctcgg gctggccacg
     3721 ccggtggcca tcatggtcgg caccgggcgc ggcgctgagc tcggcctgct gatcaagggc
     3781 ggcgaggcgc tcgagaacgc ccatcgcgtg acggccgtag cgctcgataa gaccggtacg
     3841 gtgacgctcg gcaaaccggc cgtcgtcgag gtcctcgcgt acggccacct ggacgaggaa
     3901 gccgcgttgg ccctggccgc gtcggccgag aggggatcgg agcacccgct cggcgccgcg
     3961 atcgtccgcg aggcggaagc ccggtcgctc gcgctatcgt cgcccgatag cctcaaggcc
     4021 ctgcccggcc gaggcatcga ggcgcgcctc ggcggcgccg aggccatcgt cggcaacgcg
     4081 aggctcatgg aggagcgcgg gatcgacgtt gggccagccg ccgccgacct tgagaggctc
     4141 gcggccgatg gcaagaccgc catgctcgta gcggcggaag gtcgcctcgt cggcctcgtc
     4201 gcggtcgccg acatcgtcag gccgacgagc cgggcggcga tcgcggcctt aagggcgcgc
     4261 ggcgtcgaga cgacgatgat caccggcgac tcgagagccg tcgccgaggc gataggccgc
     4321 gaggtaggag tcgaccgagt cgtcgcggag gtgctgcccg aggacaagtc ggccgcggtg
     4381 cgggcgctcc aggcctcagg caagctggtg gcgatggtcg gggacggcat caatgacgcg
     4441 cccgccctgg cggcggccga cgtgggcata gcgatagggt cgggtaccga catagcggcc
     4501 gagtcggccg atatcgtgct ggcccgcgga gacctcgagg acgccgtcgc cgccctcgac
     4561 ctgtcccgcg cgacgatgcg cgtcatcagg cagaacctgt tctgggcctt cggctataat
     4621 gtcctcggca taccggtggc ggcgggcctg ctccacctgt tcggcgggcc cctgctcaac
     4681 ccgatgatag ccgccgcggc catgtccctg tcgtcggtgt cggtcgtcac gaacgcattg
     4741 cgcctcaaga cgtggaagcc gatctagcaa gacaacgaaa ggaaccctgt atggatacgt
     4801 gcgataacca ggccgatggt tgctcgtgca agaaaaccga gcggcccgag gagcttaagc
     4861 gatcgctcga cggccgactg gccaggatcg agggccaggt gcgcggcctc cggcgcatga
     4921 tcgagggcga cgcctattgc gacgacgtga tgacgcagat cgccgccgcc cgcggcgcgc
     4981 tcggcaaagc gtccctcgtc gtgctcgagc accacctcaa gcactgcctg atcgaccacg
     5041 tgcgggccgg cgacgacgac atcgtggacg agctgctcga cagcctggcc aagaatttct
     5101 aagcgctcga cgaaccggac gggccttcct gccgcagccc ggcgcgtccg tcatactggg
     5161 cggcggctac ggcttctcgc agctcgccga cgcggcttgg ctccgcggcc cgcgcctccg
     5221 gtactggggc gacctcgaca cccacggctt cgccatcctg gatcagttca ggagcgcctt
     5281 ccccgaggcc gagtccttcc tgatggaccg cataacgttg ctcgagcacc gggagccatg
     5341 gacgacggaa cctactccga cgcgggccga gctgagccgc ctctctcccg aagaggccgg
     5401 cctctacgac gacctccgct acgacagcct gtcggcgtcg ctacgtctcg agcaggaatg
     5461 gctacgcttc tcctgagtcg aggccgcgct cgacggcgcc gcgcgctgag ctcgggtccc
     5521 gctactcgcc gaacctcggg tcgcgcagcg acagcgcctc cgagctcctc ctggccttct
     5581 cgagcgaccg tgagacgagc ggtaccgaca gtgcgacgac gtgtctgacc ggcctgcgcg
     5641 acgggtcgga gccgcgcagc ctagccgcct cgtcggcggc gcgggcttcc tcggccgtcc
     5701 agggcaggaa ggccatcgtc agcgacgcgc tcctggcgac gcggccgccg agccgattcc
     5761 cgagcggccg cagtatcgtt atcagcgcgt cgcgtagctc cacgggactc atgccaccgg
     5821 acagccaggc cgcggacgcc aatacaaggg ccagacgcac gctccgcgac agcgccggaa
     5881 cccaggcggc cagtaccgcg ccgaacccag cttcg
//
LOCUS       DAOPHV010000090         5888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_17793_length_5888_cov_4.029659, whole genome shotgun sequence.
ACCESSION   DAOPHV010000090 DAOPHV010000000
VERSION     DAOPHV010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17793_length_5888_cov_4.029659"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLQ29_03680"
     CDS             <1..196
                     /locus_tag="PLQ29_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPG85778.1"
                     /translation="RLEHAVKGIDIGAENLQAAESRIRDTDMASEMVHYTKDRILVQA
                     SSAMLAQANQKTASVLQLLQ"
     gene            451..1299
                     /locus_tag="PLQ29_03685"
     CDS             451..1299
                     /locus_tag="PLQ29_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPG85779.1"
                     /translation="MIINHNMSAMYANRSLGVTGVELNKNMEKLASGQRINRAGDDAS
                     GLAVSEKLRGQVRGLNQAQRNIENGVSFIQTTEGYLQETQDILHRVRELAVQSANGIY
                     TAEDRMQIQVEVSQLVDEINRIASHAQFNGMNILTGRFSKDGGNIMQLQVGANMDQNE
                     RIFIGTMTATALGLQGAQGTDGMISIEDPEKANASIGNLDAALKTVSKQRADLGAYQN
                     RFEMASKGVAIAAENLQAAESRIRDTDMASTMVDYVKNQILSQAGGAMLAQANTRTQS
                     VMQLLG"
     gene            1477..1809
                     /locus_tag="PLQ29_03690"
     CDS             1477..1809
                     /locus_tag="PLQ29_03690"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HPG85780.1"
                     /translation="MAIDVSAVYTLPNAASALQQEAAKPRAKQGDVMRTEPPVSMDTI
                     IEELVDVSAAFNKRLSFSLNEKLGQVVVKVIDTDTDKVIREIPPAELQHVNERIREVI
                     GILFDERA"
     gene            1835..3838
                     /gene="fliD"
                     /locus_tag="PLQ29_03695"
     CDS             1835..3838
                     /gene="fliD"
                     /locus_tag="PLQ29_03695"
                     /inference="COORDINATES: protein motif:HMM:NF005188.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HPG85781.1"
                     /translation="MSDISIPGVKSKYDTAKIIEGLMAVERIPLTRSQARLEEIRLQR
                     TVWLDLNRRMSSLRESARSLFSFQNPFNERTASSSDESVLTGTATREAIEETKSFVVK
                     RTASADRFMSDAVPADAKVPKGEYSFSVGDKTIRLSYGGGSIKDFADAINRKGAETVR
                     AQLVAIRPDERVLVIESLKTGKDNRLGFGGAAEDFALSVGLVEKASSTLQALPTDNPV
                     RFDQPLDASKAFQKDDALYLAPGAEAALKLPSPSRSEGLVLELEVELRDLPGGQAAPV
                     GPPPGPAIPSTGTIEYEGIRIASAPSEAELPAWSPPPTPPRRDDLAPLYLIDGSGRSV
                     RLPEVRPGDGFKTITVPLPAYVDSFSGVGLRNGNTGRELRVRSARVYDPTETAGLRPK
                     NPVQTAGDAVVVMDGIEVTRSDNAVSDLVPGLTINLWQASDKPVKLKVEPDREAAKEA
                     VIELVGNYNRLMAELNILARKDESVLAEIEYFTADEKKAYAERLGLFQGDTTLSQLRT
                     SMQRTMMDAYPTGGGMSQLAAFGITTDSRRGGSYDAARLRGYLEIDESALDKALQTSF
                     GRVKDLFGFDSDGDLLMDAGAAFKLDAMMKAYVETGGVVTTRTTTLDSQISRQQKEIE
                     TLEDKLAKKEAELKRQYGQMEGALGQMESTSSAWDTFNSSQGK"
     gene            3858..4724
                     /locus_tag="PLQ29_03700"
     CDS             3858..4724
                     /locus_tag="PLQ29_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85782.1"
                     /translation="MEFLMNGTAAEAAITGRTIGDVLAELDERAEAMGAIIVEVDLNG
                     RSLSPDELAEACAGSADAPGTISLVAESAAGLKARGLESLLELVRAASEAAASEDAEA
                     VASARAAWASFQAAFGGIFSAEEASFVEAFGERLGHDAPTVELTRLADGMVAFFGERL
                     AELRDPEEAMLSAARIFDAIKDDLSEVPVRLQTGKDAEAMRTMVLAVELINKTVRIMP
                     EYARASARTPTDIAGMAVPEFYGQLNGVLKELAQAFEAKDGVLIGDLAEYEIRPRLDS
                     FYAAVRAAVGDR"
     gene            4721..5824
                     /locus_tag="PLQ29_03705"
     CDS             4721..5824
                     /locus_tag="PLQ29_03705"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPG85783.1"
                     /translation="MMPAFEARALLAQVYWKQENPAESKYFLIIAGALFLILIGYSII
                     KNAVADKGAGLGQRRPQNLSGGAFRRRAEEAGFSSAEAEFLEFYARKLGVTSPQSVFG
                     SKTLLDAFMRNAFKYIERHAETEDMAEEQKHRLFAIREALGSRSSSGSAPRSTRQLKP
                     KTPLSIVTSKEAHYSSILIVNESRAMYLEPALDAFGSPIRIQYGSKLTLYFYSGNHVG
                     YSFQSRSRGMVDIDGKKFLSIAHSDRIKPLPARRNQRSEVRLSGRFYLVHVRAARDKG
                     KVVKTVQVERAAVAGIITDLSGGGLSLQTMSPANVGEFIKVEFDLGTGQRSAYATVVR
                     VSKTRNGALMHLKFVKASRKTINEIRSVVYGYD"
BASE COUNT         1171 a         1946 c         1878 g          893 t
ORIGIN      
        1 caggctcgaa cacgccgtca agggcatcga catcggcgcg gagaacctcc aggccgccga
       61 aagccgcatc cgcgacacgg acatggcgtc ggagatggtc cactacacga aggaccgtat
      121 cctcgtccag gcctcgagcg cgatgctcgc ccaggcgaac cagaagaccg cttccgtctt
      181 gcagttgctc cagtaaggct gtatactaaa aaaagatagg gccgtcccct cccgaccggg
      241 aaagggacgg ccgacggatc tttgacaaac accctcgtgc gcatgccggc ggagatgccg
      301 gccctcccct cagggagggc cggttccccc gccgtatggg gcatgaacgg acgggaacgg
      361 gcgcgagaac acggaatccc ggcctcgacg ccatgcgcgc gggacgcgcg gcaaggaatg
      421 ccgcgcagta cgtcctgcaa ggagggtcat atgatcatta accacaacat gagcgcgatg
      481 tacgccaaca ggtcgctagg cgttaccggg gtcgaactca acaagaacat ggagaagctc
      541 gcctccggtc agcgcatcaa ccgcgccggc gacgacgcct cgggcctcgc ggtttccgag
      601 aagctccgcg gccaggttcg cggcctcaac caggctcagc gcaacatcga gaacggcgtt
      661 tcattcatac agaccacgga gggttacctc caggagacgc aggacatcct gcatcgcgtg
      721 cgcgaactgg ccgtccagtc ggccaacggc atctataccg ccgaagaccg catgcagatc
      781 caggtcgagg tttcccagct ggtcgacgag atcaaccgga tcgccagcca cgctcagttc
      841 aacggcatga acatcctgac cggccgcttc tcaaaggacg gcgggaatat catgcagctg
      901 caggtcggtg ccaacatgga tcagaacgag aggatcttca taggcaccat gaccgccacg
      961 gctctcgggc tacagggcgc tcagggtacg gacggtatga tctccatcga ggatccggag
     1021 aaggccaacg cctccatcgg caacctcgac gccgccctca agacggtttc gaagcagcgc
     1081 gccgacctcg gcgcctacca gaaccgcttc gagatggctt ccaagggcgt cgcgatagcc
     1141 gccgagaacc tccaggccgc cgagagccgc atccgcgaca ccgacatggc ctcgacgatg
     1201 gtcgattacg tcaagaacca gatcctgtcc caggccggtg gggcgatgct cgcccaggcc
     1261 aataccagga cccagtccgt gatgcagctg ttgggttaat acaaccgttc atagattcat
     1321 tgcggagagc ttcctggacg tcgtctttcc gcgacggagt ctgatctgac cggaaggggg
     1381 gggatcgcgc cctcccccct tcatctttac gaaccgggcg cgcccccgcg acagggagtc
     1441 ggaacgggag cgctcccatg agacaggagg aaacgtatgg caatcgacgt ttctgccgta
     1501 tatacgctcc ccaacgccgc gtcggccttg cagcaggaag ccgctaagcc gcgcgccaag
     1561 cagggcgacg tgatgaggac cgagcctccc gtatcgatgg ataccatcat cgaggagctc
     1621 gtggacgtca gcgccgcgtt caacaagcgg ctatcgttca gcctgaacga gaagctcggc
     1681 caagtcgtcg tcaaggtaat cgataccgat accgacaagg taataaggga aattccgccc
     1741 gccgagcttc agcacgtaaa cgagcgtata cgggaggtaa tcggcatcct gttcgacgag
     1801 agggcgtaac ccggaaggag ggcctcgagg ccccatgtcg gatatcagca tccccggcgt
     1861 caagagcaag tacgataccg ccaagataat cgaaggcttg atggccgtcg agcgcattcc
     1921 gctcacgcgc tcccaggccc gcctcgagga gatacgcctc cagcgtacgg tctggctcga
     1981 cctcaaccgc cgcatgtcgt cgctccggga gagcgctcgt tcgctcttct ccttccagaa
     2041 ccccttcaac gaacgcacgg ccagctcgag cgacgagtcg gtattgaccg gcaccgccac
     2101 cagggaggcg atcgaggaga cgaagtcctt cgtcgtcaag cggacggcga gcgccgaccg
     2161 tttcatgtcg gacgccgtac ccgccgacgc caaggtgccc aagggagagt attccttctc
     2221 ggtaggcgac aagacgatac gcctgtcata cggcggcggg tcgatcaagg acttcgccga
     2281 cgcgatcaac cgcaagggcg ccgagaccgt gcgcgcccaa ctcgtcgcga tacggcccga
     2341 cgaacgggtg ctcgtcatcg agtcgctcaa gaccggcaag gacaaccggc tgggcttcgg
     2401 cggcgccgcg gaggacttcg cgctatcggt cgggctcgtc gagaaggcca gctcgacgct
     2461 ccaggcgctg ccgaccgata acccggtccg cttcgatcag ccgctcgacg cctccaaggc
     2521 gtttcagaaa gacgacgccc tctacctggc gcctggcgcc gaggccgcgc tcaagctgcc
     2581 ctccccgagc cggagcgaag gcctcgtcct cgagttggag gtcgagctcc gcgacctccc
     2641 gggcggtcaa gcggctcccg tcgggccgcc gccgggccca gccatcccct cgaccgggac
     2701 catcgagtac gagggtatac gcatcgcctc ggccccgtcg gaagccgagc taccggcctg
     2761 gtctcccccc ccgacgccgc ccaggcgcga cgacctcgcg cccctgtacc tgatcgacgg
     2821 atctggccgc tccgttcggt tgcccgaggt tcgacccggc gacggcttca agacgatcac
     2881 cgtgccgcta ccggcctacg tcgactcgtt ctcgggcgtc gggctacgga acggtaatac
     2941 cggccgcgag ctacgcgtgc gctcggcgcg ggtctacgac ccgacggaga cggccggcct
     3001 gcggcctaag aacccggtgc agacagccgg cgacgccgtc gtcgtcatgg acggcatcga
     3061 ggtgacccga tcggacaacg ccgtctccga cctcgtgccg ggcctcacga tcaacctctg
     3121 gcaagcctcc gacaagccgg tcaagctcaa ggtggagccc gaccgcgagg ccgccaagga
     3181 agcggtcatc gagctcgtag gcaattacaa taggctgatg gccgagttga acatcctcgc
     3241 ccgcaaggat gagagcgtgc tggcggagat cgagtacttt accgcggacg agaagaaggc
     3301 ctacgccgag aggctcggcc tgttccaggg agacaccacg ctatcccagc tacggacgag
     3361 tatgcagcgc acgatgatgg acgcctaccc gaccgggggc ggcatgtccc agctggccgc
     3421 cttcggcatc accaccgact ccagacgcgg cggctcctat gacgcggcca ggctcagggg
     3481 ctacctcgag atcgacgagt cggctctcga caaggccctg cagacgagct tcggccgggt
     3541 caaggaccta ttcggcttcg actcggacgg cgacctgctc atggacgccg gcgcggcctt
     3601 caagctcgac gccatgatga aggcctacgt ggagaccggc ggcgtcgtca cgacgaggac
     3661 cactaccctc gactcgcaga tcagccggca acagaaggag atagagacgc tcgaggacaa
     3721 gctcgccaag aaggaagccg agctcaagcg tcagtacggt cagatggaag gcgcgctcgg
     3781 acagatggaa agcacctcga gcgcatggga tacgtttaat tcgagccaag gcaaatgatg
     3841 atgggtagga gcgatcgatg gaattcctaa tgaatgggac ggccgccgag gcggccataa
     3901 ccgggcgcac gataggcgac gtactggccg agctcgacga gagggccgag gctatgggcg
     3961 ccatcattgt cgaggtggac ctgaacggcc gctcgctgag ccccgacgag ctggcagagg
     4021 cctgcgccgg gagcgccgac gcgcccggga cgatcagcct ggtcgccgag agcgccgccg
     4081 gtctcaaggc gcgcggcctc gagtcgctgc tcgagctggt gcgcgccgcc tccgaggcgg
     4141 cggcctccga ggacgccgag gccgtcgcgt cagctcgggc cgcctgggcg tcgttccaag
     4201 ccgcgttcgg cggcatcttc tccgccgagg aggcgtcctt cgtcgaggcg ttcggcgagc
     4261 gcctcgggca tgacgcgccg acggtcgagc tgacccgcct ggccgacggc atggtcgcct
     4321 tcttcggcga gcgactcgcg gagctacgcg acccggagga ggcgatgctt tccgcggccc
     4381 gcatcttcga cgccatcaag gacgatctgt cagaggtgcc cgtacgccta cagaccggca
     4441 aggacgcgga ggccatgcga acgatggtgc tcgccgtcga gctgatcaac aagaccgtac
     4501 ggatcatgcc ggagtacgcc cgcgcgtcgg ccaggacgcc gaccgatatc gccgggatgg
     4561 cggtacccga gttctacggt cagctgaacg gagtgctcaa ggaactggcc caggccttcg
     4621 aggccaagga cggcgtgctc atcggcgact tggcggaata cgagatcagg ccgaggctcg
     4681 attcattcta tgccgccgtc agggcggccg tcggggatcg atgatgccgg cgttcgaggc
     4741 tcgcgccctc ttggcccagg tctattggaa gcaggagaac ccggcggaga gcaagtactt
     4801 cctgatcatc gccggagcct tgttcctgat cctcatcggt tactcgatca tcaagaacgc
     4861 cgtcgccgac aagggcgcag gactcgggca gcggagaccg cagaacctat ccggaggcgc
     4921 gttcaggagg cgcgccgagg aagctggctt ctcctcggcg gaggccgaat tcctcgaatt
     4981 ctacgcccgg aagctgggcg tgacgagccc gcagtcggtg ttcggcagca agacgttgct
     5041 cgacgctttc atgcgcaacg ccttcaagta catcgagcgc catgccgaga ccgaggacat
     5101 ggccgaggag cagaagcata ggctgttcgc catacgcgag gccctcggct cccgctcgag
     5161 ctcagggtcg gccccgcgct cgacgaggca gctcaagccg aagacgccgc tgtcgatcgt
     5221 cacatccaag gaggcccact actcgtcgat cctgatagtc aacgagtccc gggccatgta
     5281 cctggaacca gccctggatg ccttcggctc gccgatacgc atccagtacg gctccaagct
     5341 gacgctgtac ttctactctg gaaaccacgt cggctacagc ttccagtccc gctcgcgcgg
     5401 catggtcgac atcgacggca agaagttcct gtccatagct cacagcgacc gcatcaagcc
     5461 gctgcccgcg aggcgcaacc agcgtagcga ggtcaggcta tcgggcaggt tctacctggt
     5521 gcacgtacgg gccgcgcgcg acaagggcaa ggtcgtcaag acggtgcagg tcgagcgggc
     5581 ggcggtcgcc ggcatcatca ccgacctctc gggaggcggg ctatcgctcc agaccatgag
     5641 cccggccaac gtcggagaat tcatcaaggt ggagttcgac ctgggcaccg gccagcgctc
     5701 ggcctacgcg acggtcgtgc gcgtcagcaa gacccgcaat ggagccctga tgcacctgaa
     5761 gttcgtcaag gcctcgcgca agacgatcaa cgagatacgc tcggtcgtct acggctacga
     5821 ctgacgcgag cctgggttgc gaaggcgccg tcgtcccgcg ttgtcccgcg ttgtcccgcg
     5881 tcgtcccg
//
LOCUS       DAOPHV010000091         5887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_17796_length_5887_cov_4.542695, whole genome shotgun sequence.
ACCESSION   DAOPHV010000091 DAOPHV010000000
VERSION     DAOPHV010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17796_length_5887_cov_4.542695"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PLQ29_03710"
     CDS             complement(<1..998)
                     /locus_tag="PLQ29_03710"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPG85784.1"
                     /translation="MQAFMDLGLNDALAKAFVSLGFEDPTEIQKLAIPAILAGRDAYV
                     SSATGTGKTFAYLAPILDSLLSSAEDSPASVQAIVVAPTHDLAVQIEREAGRLAEAAG
                     FKLRAMCALGSVPLKRQLSRLADKPQLIVGSAGRIRDLALGRYIDLSSCRWAVLDEAD
                     RLFENEAIDVTTELLSALPDACGRLLVSASLPNRTVDRSAAWFRNPLRLIVDPSEALR
                     TSIEHWCFHSVSRSKLDFLRRFEAAVKPERCLLFASSNASIFNIQKRLDYLGFPAVVL
                     KSDKEGEERKNALADFTSGAARWMITTDLGARGLDLPDVSHVISFDLPEEPTVYMHR"
     gene            1106..2035
                     /locus_tag="PLQ29_03715"
     CDS             1106..2035
                     /locus_tag="PLQ29_03715"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002679668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HPG85785.1"
                     /translation="MNLEAFILGCGGMMPLPHRHLTSVLLRREGDLFLFDAGEGTQVA
                     LRRLNLRWKKISAIFISHTHADHVTGLPGLLMLSSQVDRDEPLYIYGPPRIAEYVETS
                     RKVLDMYINYEIVVKEVQAPAVCYEGDGFVVRTFPLRHTKTCYGYAMEESPRPGAFHP
                     ERATELGVQRGPLWSRLQAGEVVQASDGREVRPEDVLGPTRSGRKFSFVTDSLYLPEI
                     APEVAGSDLFVCEGMFERELLQSAVEKKHMAAFQAATIARDAGGVKKLALIHYSPRYA
                     DQELRVLQDEARAIFPETVLSKDRMRFSLDYVD"
     gene            2100..3068
                     /locus_tag="PLQ29_03720"
     CDS             2100..3068
                     /locus_tag="PLQ29_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG85786.1"
                     /translation="MVEVSALVKRYGSFEAVRGVSFSVGSGKVLGLLGPNGAGKTTIM
                     KVLTGYHRPSSGRAVLDGRNVVEDPEGVKASLGYLPEGVPLYLDMTVAEYLDFVAAAR
                     GLGGKERRAARKAAIERAVDACGLSGVAGIRLERLSKGFRQRVGLAQAIVHDPAILIL
                     DEPTTGLDPNQILEIRSLIRSLGAEKTVILSTHILQEVEALCSEVIILNDGLVVAMGS
                     AEAIGAGLQGDERLECAVKGGFDPVEALAAIRAIPNVLSAEPTQAASGETTRFAVTAG
                     KGRGDDVAEGLFDWAAANGTKLIELKRERLSMEDIFVRLTREEPKR"
     gene            3065..3787
                     /locus_tag="PLQ29_03725"
     CDS             3065..3787
                     /locus_tag="PLQ29_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG85787.1"
                     /translation="MSGALTIAKRELSAYFNSPVAYIFIVAFLLSCGTLFFFVGDFFA
                     AGQATMRGYFSLMPFVMSVLVPALTMRLWAEERKQGTYELLLTMPLMDGQLVVGKYLA
                     ALAVVAAALALSLPVPLLASMFGRFDSGTIVAEYLGALLMASAASAIGQAVSSASKNQ
                     ISAFMATVMLLLALSLMSRLTALLSLPGWLAGLVNWVSLSYHFGSFSRGVIDTRDVAY
                     FCVITVSCLYATARLISAGKWR"
     gene            3791..5404
                     /locus_tag="PLQ29_03730"
     CDS             3791..5404
                     /locus_tag="PLQ29_03730"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HPG85788.1"
                     /translation="MIETLRKRERLLLALIVVGLVLAAVASTMYYARLDLTSTRMFTL
                     SDAAKALRNEIPESVRISYFVSKSLADRHPGPGEIEDFLRELESVSKGKVRVRVVDPS
                     DDPTEVDGFGVAAQQMQVVERSEQRVAIVYTGIVVEYLDRHETIPSVLSTATLEYELV
                     KAVRAVVSNDVQVIGLLVGDADKSLAGDYQTLRGNLARAGYETRELERGRAVDDDVDV
                     LFVLGNAAMDRYDSAFVDAFVMRGGKTFFAVKGVDVRADYGLAASAVPDGGLLSMLSS
                     YGFDVDRSLVLDQSSLTVPFQTQGPTGSYQIQYLRYPFWIIVDERFVSDDSPMTARFG
                     GLDLFWPSPLRLRPVEGVEYVELAKTSPKAWLQTERFAAGPDDQALYALERDGTGGQY
                     LLAASASGSFRSAFSAGDPPYRDGAPPLAPTRVESEPTRMVVVSSADFLSDLMRMSDS
                     GFNASFALNAADWLASSDDLIAIRSRAVADTRLNRIDDDGLRDFLVAVTYIVCLGIVP
                     FGVVAFAFARSARRRGAEAASRPSRGGAA"
     gene            5401..>5887
                     /locus_tag="PLQ29_03735"
     CDS             5401..>5887
                     /locus_tag="PLQ29_03735"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HPG85789.1"
                     /translation="MIDYTKRVRLVAAADAALALLVSLGLILSPTSSGSKESRRDLLD
                     DASAVTSIRISGGEPVELVKTGTGWEAVDGDAWLPVDSSRVAAFLKAVDSIERAELVA
                     EDASSQPALGLGAGARKVTLLGEGGKELCAFALGSYASSPGTVYISIGDGPESYAVAS
                     GM"
BASE COUNT          960 a         1869 c         1993 g         1065 t
ORIGIN      
        1 cgatgcatgt agacggtcgg ctcctcgggc aggtcgaagc tgatgacgtg ggacacgtcc
       61 ggcaggtcga ggccgcgagc gcctaggtcg gtggtgatca tccacctggc ggctccgctc
      121 gtgaagtcgg ccagggcgtt cttgcgttcc tcgccctcct tgtcggactt gaggacaacc
      181 gcgggaaagc ccaggtagtc cagccgtttc tggatgttga agatggacgc gttcgacgag
      241 gcgaatagca ggcagcgctc gggcttgacc gccgcctcga agcgccggag gaagtcgagc
      301 ttcgatcggg acacggagtg gaagcaccag tgctcgatgc tcgtgcgtag cgcttcggag
      361 gggtcgacga tgagcctgag cgggttccgg aaccaggccg ccgaccggtc gacggtgcgg
      421 ttcggcaggg atgccgagac gaggagccgc ccgcaggcgt cgggcagagc cgataagagc
      481 tcggtggtga cgtcgatggc ctcgttctcg aacaggcggt cggcctcgtc gagcacggcc
      541 cagcggcacg acgacaggtc gatgtatcgg ccgagcgcca ggtcgcgtat gcggccggcg
      601 gagccgacga tcagctgggg cttgtcggcc agccgcgaca gttgccgttt gagcggtacc
      661 gagccgaggg cgcacatggc tcgcagcttg aagccggcgg cctcggcgag gcgaccggcc
      721 tcgcgttcga tctggacggc caggtcgtgg gttggcgcta cgacgatggc ctggaccgag
      781 gccggggagt cttccgccga ggataggagg gagtcgagta tcggcgcgag gtacgcgaag
      841 gtcttgccgg taccggtcgc ggagctgacg taggcgtccc tgccggcgag tatggccggg
      901 atggccagct tctggatttc agtcggatct tcgaatccaa gggagacgaa ggccttggct
      961 aaggcatcgt tcagcccgag gtccataaat gcttgcatct taatatattc gcataaaagc
     1021 ggagcgatac gcaatagtcg ggcgagcgtg acgtcacgac ttctccgttg catcgtatgc
     1081 cgctttttac gtatagtagt cccacatgaa cctagaagcc tttatcctcg gctgcggcgg
     1141 catgatgccc ctgccgcatc ggcaccttac cagcgtcctt ctcaggcgcg agggcgacct
     1201 gttcctcttc gatgccgggg agggtacgca ggtggccctc cggcggctta acctccgctg
     1261 gaagaagatc agcgccatct tcatctcgca tacccacgcc gaccacgtca ccggcctgcc
     1321 tggcttgctc atgctgtcga gccaggtcga ccgggacgag cccctgtaca tctacggccc
     1381 tccgcgtata gccgagtacg tcgagactag ccgtaaggtg ctcgacatgt acattaacta
     1441 tgagatcgtg gtcaaagagg tacaggcgcc ggccgtctgc tacgagggcg acggcttcgt
     1501 cgtcaggacc ttcccgctca ggcataccaa gacctgctac ggctacgcca tggaagagtc
     1561 gccgcgtccc ggcgctttcc acccggagag ggctaccgag ctgggcgtcc agcgtggacc
     1621 gctctggtcg aggctgcagg ccggcgaggt cgtgcaggcc tcggacggcc gggaggtcag
     1681 gccggaagac gtgctcggcc cgacgaggag cggcaggaaa ttcagcttcg tcaccgactc
     1741 gctgtacctg cctgagatcg cgcccgaggt ggccggctcc gacctgttcg tctgcgaggg
     1801 catgttcgag cgggagctat tgcagagcgc cgtcgagaag aagcatatgg cagccttcca
     1861 ggccgcgacc atcgcccgcg acgcaggcgg ggtcaagaag ctggccctga tccactatag
     1921 cccgcgctac gccgatcagg agctgcgcgt actgcaggac gaggcgcggg cgatattccc
     1981 cgagaccgta ctctccaagg ataggatgcg cttctcgctc gactacgtgg actgacgcgg
     2041 agcggtaacc acccgaaccc cgggttgttt cagtcccgga ttatgttctg gaggctcgta
     2101 tggtagaagt atcggctctc gtcaagcgct acggctcctt cgaggccgtg cgcggcgtga
     2161 gcttctccgt cggctcgggc aaggtgctcg gcctgctcgg cccgaacggg gctggcaaga
     2221 cgacgatcat gaaggtgctg accgggtatc accggccgag ttccggtcgc gccgtcctcg
     2281 atggacggaa cgtcgtcgag gatccggagg gagtgaaggc ctccctaggc tacctgcccg
     2341 agggtgtacc cctctacctc gatatgaccg tggccgagta tctcgacttc gtcgccgccg
     2401 cccgcgggct cggaggcaag gagaggcgcg ccgccagaaa agccgcgatc gagcgggccg
     2461 tcgacgcctg cggcctgtcg ggcgtagcgg gcattcggct cgagcggcta tccaagggct
     2521 tcaggcagcg cgtcggactg gcccaggcca tcgtgcatga tccggccata ctcatcctcg
     2581 acgagccgac gaccggcctc gacccgaacc agatcctcga gatccgttcg ctgatccgtt
     2641 ccctcggggc ggagaagacg gtcatactgt cgacccatat actccaggag gtcgaggcgc
     2701 tctgctccga ggtgatcatc ctcaacgacg gactcgtcgt cgccatgggt agcgccgagg
     2761 ccataggcgc gggcctgcag ggcgacgaac ggctcgaatg cgccgtcaag ggcggtttcg
     2821 accccgtcga ggctctggcg gctatacgcg ccatcccgaa cgtcctgtcc gcggagccga
     2881 cccaagccgc gagcggcgag acgacccgat ttgccgtgac cgccggcaag ggccggggag
     2941 acgacgtcgc cgaggggctg ttcgattggg ccgccgccaa cggcacgaag ctcatcgaac
     3001 tcaagcgcga gcgcctctcg atggaggata ttttcgtgcg actgaccagg gaggagccaa
     3061 agcgatgagc ggcgccctta ctatagccaa gagggaactg tcggcctatt tcaactcgcc
     3121 ggtggcctat atcttcatcg tcgcgttcct gctctcttgc gggacgctgt tcttcttcgt
     3181 cggcgatttc ttcgccgccg ggcaggcgac gatgcgcggg tacttctcgc tgatgccgtt
     3241 cgtcatgtcg gtcctcgtcc cggcgctgac gatgaggctc tgggccgagg aacgcaagca
     3301 gggtacctat gagctgctcc tgaccatgcc attgatggac ggacagctcg tcgtcggcaa
     3361 gtacctcgcg gccctggccg tcgtggccgc cgcgctcgcg ctgagcctgc cggtgccgct
     3421 cctggcctcg atgttcggcc gcttcgactc aggcaccata gtcgccgagt acctgggcgc
     3481 cctcctgatg gcctcggccg cctcggccat aggccaggcg gtgtcgagcg cgtcgaagaa
     3541 ccagatcagc gccttcatgg ccacggtgat gctgctcctg gcgctcagcc tcatgtccag
     3601 gctcaccgcg ctcctatcgc tgcccggctg gctggccggc ctcgtcaact gggtgtcgct
     3661 gtcgtaccat ttcggctcgt tctcgagagg tgtgatcgat acgagggatg tggcgtactt
     3721 ctgcgtcata accgtatcgt gcctctacgc cacggccagg ctcatctcgg ccgggaagtg
     3781 gaggtaggcg atgatcgaaa cgcttcgtaa acgggagcgc ctgctcctcg ccctgatcgt
     3841 cgtcgggctg gtcctcgcgg cggtggcttc gacgatgtat tacgctcggc tcgacctgac
     3901 ctcgacgcgc atgttcacgc tgtcggatgc ggctaaggcc ctgcggaacg agataccgga
     3961 gagcgtgagg atcagctatt tcgtctcgaa gagcctggcc gaccgccatc ccggacccgg
     4021 cgagatcgag gacttcctgc gagagctcga atcggtcagc aagggcaagg tgcgcgtgcg
     4081 cgtcgtcgac cctagcgacg acccgaccga ggttgatggc ttcggcgtgg cggcgcagca
     4141 aatgcaggtg gtcgagcgct cggagcagcg cgtggccatc gtctataccg gaatcgtcgt
     4201 ggagtacctg gaccgccatg agaccatacc gtccgtgctg tcgaccgcca cgctcgagta
     4261 cgagctggtc aaggccgtca gggcggtcgt atcgaacgac gttcaggtga tcgggcttct
     4321 cgtcggcgac gccgataaga gcctggcggg ggactatcag acgctacggg gcaacctcgc
     4381 gagggccggc tacgagacgc gcgagctgga gcgcggccgg gccgtcgacg acgacgtcga
     4441 tgtgctgttc gtactcggta acgcggcgat ggatcgttac gactcggctt tcgtagacgc
     4501 cttcgtcatg cgcggaggca agaccttctt cgcggtcaag ggtgtcgacg tgcgggccga
     4561 ctacgggctg gcggcgtccg cggtaccgga cggcggcctc ttgtcgatgc tgtcgtccta
     4621 cggcttcgac gtggatcgct cgctggtcct cgaccagtcg agccttaccg tgccgttcca
     4681 gacccagggg ccgaccggct cgtaccagat ccagtacctg cgctacccgt tctggatcat
     4741 cgtcgacgag cgtttcgtat cggacgactc gccgatgacg gcccgcttcg gcggtctcga
     4801 cctgttctgg ccgagcccgc tacgcctccg acccgtcgaa ggcgtcgagt acgtcgagct
     4861 ggccaagacc agcccgaagg cctggctgca gaccgagcgc ttcgccgccg gtccggacga
     4921 ccaggccctg tatgcgctcg agcgcgacgg cacgggcggt cagtacctgc tcgcggcctc
     4981 ggcgagcgga tccttcagga gcgccttctc ggccggagat ccgccgtaca gggacggagc
     5041 cccgccgcta gcgccgacga gagtcgagtc cgagccgacc cgtatggtcg tcgtctcgtc
     5101 cgcagacttc ctaagcgacc tgatgcgcat gagcgactcg ggcttcaacg cgtcgttcgc
     5161 gctgaatgcg gccgattggc tcgcctcgag cgacgacttg atcgcgatcc ggtccagggc
     5221 cgtcgccgac acgcggctca accgcatcga cgacgacggc ctgcgggatt tcctggtcgc
     5281 cgttacctat atagtctgcc tcggcatcgt gccattcggc gtcgtggcct tcgcctttgc
     5341 gcgctcggcg cgcaggcgcg gcgccgaagc cgcctcgagg ccttcgaggg gaggagcggc
     5401 atgatcgact atacgaagcg cgtacggctc gtcgccgcgg ccgacgcggc gctggccctg
     5461 ctcgtgtcgc tcggcttgat actatccccg acttcgtctg gctcgaagga gtcgcggcgc
     5521 gacctcctcg atgacgcgtc tgccgtaacc tcgatacgca tatccggggg cgagccagtc
     5581 gagctcgtca agacaggtac cggctgggaa gcggtcgacg gcgacgcgtg gctaccggtg
     5641 gattcatccc gtgtggctgc ctttctgaag gcggtcgatt cgatcgagcg ggccgagctt
     5701 gtcgccgagg acgcttcgtc gcagccggcc ctcggcctcg gcgcgggagc gcgcaaggtg
     5761 actctgctgg gcgaaggcgg gaaggagcta tgcgcgttcg ccctcggttc gtacgcttcg
     5821 tcgcccggta cggtctatat atcgataggc gatgggccgg agtcgtacgc cgtcgcctcc
     5881 ggcatgg
//
LOCUS       DAOPHV010000092         5869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_17879_length_5869_cov_4.072583, whole genome shotgun sequence.
ACCESSION   DAOPHV010000092 DAOPHV010000000
VERSION     DAOPHV010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17879_length_5869_cov_4.072583"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PLQ29_03740"
     CDS             complement(<1..499)
                     /locus_tag="PLQ29_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85790.1"
                     /translation="MNVHARLAIVSAVLLAALASSCAGREGWALVLWPPEGSALGYGD
                     VAPIHFKSNITKTYAVGVPDSDAKEELELWRVERYPSKKKAREAADGYGDLAPVLGVA
                     MRDGLILRTEPSNVRSEQVYRLRLGQPVKLLRRVEGATVETGGERLEAGAIANHLRKA
                     LQPGFV"
     gene            600..2132
                     /locus_tag="PLQ29_03745"
     CDS             600..2132
                     /locus_tag="PLQ29_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPG85791.1"
                     /translation="MSVRTEAVIEIGSTGIRVLVANVSDDGTYEILERAGKPIALGRD
                     VFTTGLVSRDLVGECIAVLRSFRELLRAYGVEPTAARVVATSALREAENRDAFVDRVA
                     MRTGFRVDVIEAIEESHYMFLAVQRALGGDAGYLSRTNSMIIEVGGGSTELMLLRRGR
                     IVAAHSLRLGTVRVDEQSKAVMGSSGYLMRFLADNVRTACDGLEDDLPLSGVRSFVAI
                     GNDARFAASVAGVEARSDYTIVPREAFLGLVDRLKGLTVDDIVSSYRVPYSDAEALLP
                     GLVILSLFIERTGAEELVVPRVSLRDGLLIAMSGGSSDDIQAELHKQIMASVIGLGRR
                     YRFDEDHASHVVEHALFLFDRLGEAHGMGRRERLLLEAAAYLHDVGNFIRTSGHQRHS
                     EYIVINSEIFGLQRDELTIVANLVRYHRKAGPSPSHANYMSLPREDRAVVLKLSALLR
                     VADALDRGHTQRIKLVDLEMKEDHLVLHSDSSGDLSLERLSLAEKDDMFEDVFGLKVV
                     LI"
     gene            2129..4264
                     /gene="ppk1"
                     /locus_tag="PLQ29_03750"
     CDS             2129..4264
                     /gene="ppk1"
                     /locus_tag="PLQ29_03750"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPG85792.1"
                     /translation="MSHKPDREFYLSRELSWLEFNARVLEEGLDGANPLLERLRFLSI
                     VSSNFDEFFMVRVAAVKARARSGDADQDYAGLGPEELLSAISRRAREIAGKQYAALTN
                     ELLPALAAEGLWLMRPAEYDVESRRWLEDYFMGQVAPALTPLAVPVPEEGSPVAFPTT
                     GNLRIHAAFLLRSPGGEEERLAIVQVPPNLGRFVRLPSGPGARYALLDDLVMDFGHRL
                     FPGYAVTERCLFKVTRDADIGVDEDRDDDFVAAMEEVLVNRQNSWPVRLTISSDSEEL
                     EARLREALGLGQDDVYALPGPIDLKSFMEIANLKGFDRLRYKPRSRFSPLALSEDNTV
                     WDEIRKRDLVLHLPYESFQPIVDFVEAAASDPDVLAIKATLYRTSGDSPVVKALTRAA
                     RAGKQVTVVVELKARFDEERNIVWASRLEQAGAIVVFGAARLKVHAKAMLVVRREEDG
                     LVRRYVHLSTGNYNDKTARLYGDLSLFTANPTICAETGAFFNMITGLSARTELRNLSM
                     APFDLKRRVISMIEREAERSSPESPGLIAAKMNSLCDKEIIDALYRASAAGVRVMLNV
                     RGICQLVPGLKGLSENITVVSIVGRYLEHARVCYFRNGGSEELYLSSADWMPRNLEKR
                     IELMFPVFGEEARDRVYDALMSYFLDNVKARALGPSGHWRRVKPLEGEAPFSVHDYFY
                     EDSLKRLERASATSATSDAKMIQVRRRAP"
     gene            complement(4245..>5869)
                     /gene="malQ"
                     /locus_tag="PLQ29_03755"
     CDS             complement(4245..>5869)
                     /gene="malQ"
                     /locus_tag="PLQ29_03755"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPG85793.1"
                     /translation="PLPIGDDLTWTKSTRAMVERVDSPIILMLLDDFFFRSRVNTARV
                     EGLVRDFERLDAAYLRLVPNPPPDLRLARFPDVGEIAPGAPYRSSLQAALWRKSDLLE
                     LLRDEELEAARDTRGGPVDYGRVIPAKERLARLAAERLLSSGRFASELDAFRLANAGW
                     LDDYCLFTVIKRERAVAAAAAGVDDSSWNAYWPRTLARREPEAIAAFRAGHGRELSLI
                     EAEQFLFRRQWDELRERAAGRGLSIVGDLPIFVAPDSADAWAHPELFRLDASGRPIEV
                     AGVPPDYFSRDGQLWGNPLYDWERHEATGFSWWIARVEAALSLYDMVRVDHFRGLAAC
                     WAVPAGRKTARRGEWKAAPGAALLSALRARLGDELPILAEDLGLITPDVVALRDSFGL
                     PGMRILQFGFDARESGRGLDPDNPFLPHTYVPGCVAYTGTHDNDTLAGWLASASQAER
                     AFVSGYLGYDPPDPVRALVREAMKSAAGLAVFPMQDLLGLGSEARMNRPGATGGNWAW
                     RLESLPGPDLADELRAMTRLYSRLPAPTAPCA"
BASE COUNT          929 a         1997 c         2005 g          938 t
ORIGIN      
        1 ccacgaagcc cggctgcagg gccttgcgga ggtggttggc gatggcgccg gcctcgagcc
       61 gttcgccgcc ggtctcgaca gtcgctcctt cgactcggcg gagcagcttg accggctggc
      121 cgagtctgag gcggtagacc tgctcggagc ggacgttcga cggctcggtc ctgagtatga
      181 gcccgtcgcg catggccacg ccgagcaccg gcgccagatc gccgtagccg tccgcggcct
      241 cccgggcctt cttcttggac gggtagcgct cgacccgcca cagttcgagc tcctccttgg
      301 cgtccgagtc cggaacgcct acggcgtacg tcttcgtgat gttcgacttg aagtgtatgg
      361 gagccacgtc cccgtagccg agcgccgagc cttcgggcgg ccagagtacg agggcccagc
      421 cctcccggcc ggcgcaggac gaggcgagcg ccgctagtag gacggccgat acgatagcca
      481 gccttgcatg tacgttcatc gatgccatgc tacattatat aacgaagggc acccgccttg
      541 tcacgcccgc ttgcgctccg gtccgggctc gggcatacta gatggctcga ggaggttccg
      601 tgagcgtcag gacagaggcc gtcatcgaga tcggctccac cgggatcagg gtcctcgtgg
      661 ccaacgtctc ggacgacggg acctacgaaa tcctcgaacg ggcgggcaag ccgatcgccc
      721 tcggccgtga cgtctttacg accggcctcg tcagccgcga cttggtgggg gagtgcatcg
      781 cggtgctccg ctcgttccgc gagctcctgc gcgcctacgg agtcgagccg acggcggccc
      841 gcgtcgtggc gacgagcgcc ctgcgcgagg cggagaatcg cgacgccttc gtcgaccggg
      901 tagcgatgcg caccggcttc cgcgtcgacg tcatcgaggc gatcgaagag agccactaca
      961 tgttcctggc cgtccagcgg gccctcgggg gcgacgccgg ctatctttcc aggaccaact
     1021 ccatgatcat cgaggtcggc ggcggctcta ccgagctgat gctgctccgc agggggcgga
     1081 tcgtcgcggc ccactcgctc aggctcggca cggtccgcgt cgacgagcag tccaaggccg
     1141 tcatgggttc gtcgggctac ctgatgcgct ttctcgccga caacgtcagg acggcctgcg
     1201 acgggctcga ggacgacctg ccgctgtcgg gcgtgcgctc gttcgtcgcc atcggcaacg
     1261 acgctcgctt cgccgcctcc gtcgccgggg tcgaggcgcg gtccgactac acgatcgtgc
     1321 cgcgcgaggc cttccttggc ctcgtcgacc ggctcaaggg cctgacggtc gacgacatcg
     1381 tctcgtcgta ccgcgtgccg tactcggacg ccgaggcgct actgccaggc ctcgtgatcc
     1441 tgtcgctgtt catcgagcgc accggcgccg aggagctggt cgtgcccagg gtcagcctac
     1501 gcgacggcct cctgatagcc atgtccggag gctcgagcga cgacatccag gccgagctcc
     1561 ataagcagat catggcgtcg gtcatcggac tcggccgccg ctaccgcttc gacgaggacc
     1621 acgcctccca cgtcgtcgag cacgcgctgt tcctgttcga ccgcctcggt gaggcccacg
     1681 gcatgggccg cagggagcgc ctcctgctcg aggccgccgc ctacctgcac gacgtcggca
     1741 acttcatccg tacgagcggc caccagcgcc actcggaata catcgtcatc aacagcgaga
     1801 tcttcgggct gcagcgcgac gaactgacca tcgtcgccaa cctcgtccgc taccaccgca
     1861 aggcgggccc atccccgtcc cacgccaact acatgagcct gccgcgcgag gaccgggcgg
     1921 tcgtgctcaa gctgtccgcc ctgctccgcg tcgcggacgc tctggaccgg ggccatacgc
     1981 aacggatcaa gctcgtggac ctggagatga aggaggatca cctggtcctc cattccgata
     2041 gctccggcga cctgtccctg gagcggctgt cgctcgcgga gaaggacgac atgttcgagg
     2101 acgtattcgg cctcaaggtg gtgctgatat gagccataag cccgaccgcg aattctacct
     2161 gagccgcgag ctgtcctggc tggaattcaa cgcccgcgtg ctcgaggaag gtctcgatgg
     2221 ggcgaacccc ttgctggagc gcctacgctt cctttccatc gtctcgagca acttcgacga
     2281 gttcttcatg gtgcgcgtgg cggccgtcaa ggccagggcc cgctccggtg atgccgacca
     2341 ggactacgcc gggctcgggc ccgaggaact gctgtcggcc atctcgcgta gggcccgcga
     2401 gatagccggc aagcagtacg ccgccctgac gaacgagctg ctgccggccc tggccgccga
     2461 gggcctctgg ctgatgcggc cggccgagta cgatgtcgag tcgaggcgct ggctcgagga
     2521 ttacttcatg ggccaggtag cgccggccct gacgccgctg gccgtaccgg tcccggagga
     2581 aggttcgccc gtcgccttcc cgacgaccgg caacctgcgc attcacgccg ccttcctgct
     2641 gcgttcgccc ggcggcgagg aggagcgcct ggccatcgtc caggtgcctc ccaacctggg
     2701 gcgcttcgtc cggctgcctt ccggtccggg agctcgctac gcgttgctcg acgacctggt
     2761 gatggacttc ggccaccggt tatttccggg ctacgccgtg acggagcgtt gcctgttcaa
     2821 ggtgaccagg gacgccgaca tcggcgtcga tgaggaccgt gacgacgact tcgtcgcggc
     2881 catggaggag gtcctcgtca accggcagaa ttcgtggccg gtcaggctga ccatctcctc
     2941 cgactccgag gagctcgagg cgcggctccg ggaagcgctc ggcctgggtc aggacgacgt
     3001 gtacgcgttg cccggcccca tcgaccttaa aagcttcatg gagatcgcca acctgaaggg
     3061 cttcgaccgg ctgcgctaca agcccaggag ccggttctcg ccgctggccc tgtcggagga
     3121 caataccgtt tgggacgaga tacgcaagcg agacctggtg ttgcacctgc cctacgagtc
     3181 gttccagccc atcgtcgact tcgtcgaggc cgcggcctcc gaccccgacg tgctagcgat
     3241 caaggctacc ctataccgga cctccggcga ctcgcccgtg gtcaaggcgc tcacgcgggc
     3301 cgcgagggcc ggcaagcagg tcacggtcgt cgtcgagctg aaggcgcgct tcgacgagga
     3361 gcgcaacatc gtctgggcgt ccaggctgga gcaagccggc gcgatcgtcg tgttcggagc
     3421 tgcccggctc aaggtgcacg ccaaggcgat gctggtcgtc aggcgcgagg aggacgggct
     3481 ggtccggcgc tacgtgcacc tgtccaccgg caactacaac gacaagaccg cccggctgta
     3541 cggcgacctg tcgctgttca cggccaaccc gaccatctgc gcggagaccg gggcgttctt
     3601 caacatgatt accgggctgt ccgctcgtac cgagctgcgg aacctatcga tggcgccgtt
     3661 cgacctgaag cgccgcgtca tatcgatgat agagcgcgag gccgagcggt ccagcccgga
     3721 gtcgcccggg ctcatcgccg ccaagatgaa ctcgctgtgc gacaaggaga tcatcgacgc
     3781 gctgtaccgg gcctccgcgg ccggcgtacg cgtgatgctc aacgtgcggg gcatctgcca
     3841 gctcgtgccg gggctcaagg gcctgtccga aaatattacg gtcgtgtcga tcgtcgggcg
     3901 atacctggaa cacgcgcgcg tctgctactt ccgcaacggc ggctccgagg agctgtacct
     3961 atcgagcgcc gactggatgc cgcggaacct cgagaagcgc atcgagctga tgttccccgt
     4021 gttcggcgag gaggcgcgcg atcgagtcta cgacgcgctg atgtcgtact ttctggacaa
     4081 cgtcaaggcc agggcgctcg gcccgtcggg gcactggcga cgagtcaagc cgctcgaggg
     4141 cgaggcgccc ttctcggtgc atgactattt ctacgaggac agcctcaagc ggctggagcg
     4201 ggcgagcgcg acgagcgcga cgagcgacgc caagatgata caggtcaggc gcagggcgcc
     4261 gtaggcgcgg ggagccggct atagagccgc gtcatggcgc gtagctcgtc ggccaggtcc
     4321 gggcccggta ggctctccag ccgccaggcc cagttgcctc cggtcgcgcc cggacggttc
     4381 atcctggcct ccgagcccag gccgagcaga tcctgcatcg ggaagaccgc caggccggcc
     4441 gcgctcttca tcgcctcgcg cacgagcgcg cgtaccgggt ccggcggatc gtagcccagg
     4501 tagccggaga cgaaggcgcg ctccgcttgg ctggccgacg cgagccagcc ggccagcgta
     4561 tcgttgtcgt gggtgccggt ataggctacg catccaggaa cgtaggtatg gggcaggaac
     4621 gggttgtccg ggtcgaggcc tcggcccgat tccctggcgt cgaagccgaa ctgcagtatc
     4681 ctcatgcccg gtagcccgaa gctatcgcgt agcgcgacga cgtcgggggt gatgaggccg
     4741 aggtcctcgg ccagtatcgg cagctcgtcg cccagcctag ccctgagcgc ggacaagagg
     4801 gccgcgccgg gagccgcctt ccactcgccg cgcctggccg tcttgcgtcc ggctggtacc
     4861 gcccagcacg cggcaaggcc tctgaagtgg tcgacgcgga ccatgtcgta gagcgatagc
     4921 gccgcctcca ccctcgctat ccaccacgag aacccggtcg cctcgtggcg ctcccagtcg
     4981 tagagcgggt tgccccagag ctgtccgtcc ctcgagaagt agtcgggcgg aacgccggcc
     5041 acctctatcg gcctgcccga ggcgtctagc ctgaacagct cgggatgggc ccaagcgtcc
     5101 gccgagtccg gcgccacgaa gatgggcagg tcgccgacga tggacagccc gcggcccgcg
     5161 gcccgctcgc gtagttcgtc ccattgccgg cggaacagga actgctcggc ctcgatcagc
     5221 gacagctcgc gcccatgccc agcgcgaaag gccgcgatgg cctccggctc gcgcctggcg
     5281 agggtgcgcg gccagtacgc gttccacgac gagtcatcga cgccggccgc cgccgccgcg
     5341 acggcgcgct cgcgcttgat aaccgtgaat aggcaataat catcgagcca gcccgcgttc
     5401 gccagcctga aggcgtcgag ctccgaggcg aaccttccgg acgagaggag ccgttcagcg
     5461 gccagccgag ccaggcgctc cttggccggg atgacgcgcc cgtagtccac cggcccgccg
     5521 cgggtatcgc gcgccgcctc gagttcctcg tcgcggagga gctcgaggag gtcgctcttg
     5581 cgccacaggg ccgcttgcag cgacgagcgg tagggggcgc cgggcgcgat ctcgcccacg
     5641 tcggggaagc gcgcgaggcg gaggtcaggc ggggggttgg ggacgaggcg gaggtacgcg
     5701 gcgtcgaggc gttcgaagtc gcggacgagg ccctcgacgc gcgcggtgtt cacccgcgag
     5761 cggaagaaga aatcgtcgag gagcatgagg atgatcggcg agtcgacgcg ctcgaccatc
     5821 gcgcgggtgc tcttcgtcca ggtgaggtcg tcgccgatgg ggagcgggg
//
LOCUS       DAOPHV010000093         5832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_18020_length_5832_cov_4.337199, whole genome shotgun sequence.
ACCESSION   DAOPHV010000093 DAOPHV010000000
VERSION     DAOPHV010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18020_length_5832_cov_4.337199"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2424
                     /locus_tag="PLQ29_03760"
     CDS             <1..2424
                     /locus_tag="PLQ29_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85794.1"
                     /translation="IDGVFLRTTREPAKSKTTDFVKVIVDLYSNLGGFAAVQAQLASP
                     GPFSSTTAFAGIGLSRSVFGAADGYTPFVSAGDYASVWNGVDLLGTELPFRFGWELAS
                     SLSAGPVKLSFSLPFYSDAYYNVDFRDRSEQMNWLQFLDQDDDETTVSKLSSFTDSLS
                     LSASLPSASLPSWLSTASLTKLNTSLAWNSVVKPTPSDSTEAKLFAVDPTREFFAPYE
                     WTVLDSSASLSGSLFKYPRKAGKAVPTASGPTAAASATDARGDEGGEGDERVPDAVDR
                     ALAALPSLEAPWATSAALEKAEKAEKAEKAAAPGFSAPALASPETYADRTPLSASMSW
                     SLSPTFAWKRRFLTTAWTEPELVDWSALYETRTSRAAGSVNLVGAAYDGLLALTLGLS
                     ASSQAQDRPSISSDAAYASESLKTTWAKQDAQYRNDKLSAQLKLTSSPFQDLWLWSPT
                     SFGYSLSSTLYEYAFKEMDATAPTDASKAVYETVTADWTDETVSAHALSLDLGLQPWG
                     YKQVLSLQADLPPRLGGYTGRLSLKAPWATLTVGSEYSMPSEDEEYVWSPLSSSLTLG
                     EAPWPKLTGSYVWDIEDEKPTALSLQVAWNGFTASASAKEAVAYRLSYGAGWSSVGDP
                     SFMVNSMSMAYKTSWKPPAAWRRRIQWTMDVNAAAQQSFLRFTDSYLNFVYGLTFKVH
                     ELLDLSLSSTSRNASLWRYFPAAFEIPEEIALDPVNPLTDLLASFNFFDGTGEARRAS
                     LFKLKALELTATHHLHDWDLALKLTASPVLENLEYVFKTDFSVTLSWNSVSQIKSSYS
                     KKGDTVSWD"
     gene            2480..3445
                     /locus_tag="PLQ29_03765"
     CDS             2480..3445
                     /locus_tag="PLQ29_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPG85795.1"
                     /translation="MVPTAAVGIALDDTRLLADVCGVNDYNLRVLERLLGVPVFVRGN
                     EISLESPRPDVRELFAAVIEQLLSSIDDGLPASPELITAIHASLTPDGQAAPSGASFK
                     DSAIQVPGAFSKVYPRTANQALYLRGLSSHDISVAVGPAGTGKTYLAVAWALREVLSK
                     ARRRLVLTRPVVEAGESLGFLPGDLGQKISPYLRPLYDAMEALVPYDVVKRLEDNHTI
                     EIAPLAYMRGRSLNNCVVILDEAQNTTKEQMKMFLTRLGEGSVAVVTGDATQIDLPRK
                     SESGLVHALKVLSDIDDIFVCRLDGRDVVRNPLVRKIIDAYESEI"
     gene            3429..5630
                     /locus_tag="PLQ29_03770"
     CDS             3429..5630
                     /locus_tag="PLQ29_03770"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPG85796.1"
                     /translation="MSQRSDSYGRLDRLRAAFSDRAMVRRYGAIAVAALVCVAAYIGF
                     GAASGAAGKGGFEAGKVADRDVVAERDVVYIDVRATKIRTEAELRLVPAVFSLDDAAT
                     RAALAELEAFRLGFASLAGEIEGREELALRLRGRFPDLAATDLPERLTNYPAPSNAIS
                     QGRAVLTALLGAGVFAIPDSGLEQYNPESVELRRWTDGPLVYEQVPLESATTTRRLKA
                     AALDIAKSLKLSATMSALAADLAVAFARENAFFDAEQSLRRLESARARVEPVMRKLAK
                     GDKVIREGFIVTAEDLERLAALRGSEMEFDALYALAGVLLLAALGVASVALLGRGSTG
                     IRVEGASYWFVLAVAVGYFVASSALSAFLPPVLAAHIDAFLPTALVSMLVAILIGERF
                     ALMFTLIIGSSLVLVTGLDAFVPAQAIASGLAGIVLVRRADKRIDLVRAGSQLAAARF
                     AIGFALAALADRGAVAALSTAFWSAVNGFLCGVLALSFLPVLEHMLNAPTVFRLQELS
                     DLNAPALKRLLSVAPGTYSHSVTVAHLAESACREIGADALLARVGAYYHDIGKIEKPE
                     YFIENQSGYNKHDEINPRLSATVLRGHVKFGLERADALRLPESVKAIIAEHHGTSLIS
                     YFFAQAQKESMEASSEEYRYPGPLPSSKEAAVVMLADCVEAASRTLKRPTIGRLEQFV
                     KELVMDRFGNGQLSMSALTFRDLELIMNSFVRVLSGHFHSRIEYPKTKEQG"
     gene            5732..5832
                     /locus_tag="PLQ29_03775"
                     /pseudo
     CDS             5732..5832
                     /locus_tag="PLQ29_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNAse YbeY"
BASE COUNT          885 a         2005 c         1975 g          967 t
ORIGIN      
        1 atcgacgggg tgttcctccg gacgacgcgg gagccggcga agtctaagac gaccgacttc
       61 gtcaaggtga tcgtcgacct gtactcgaac ctgggcggct tcgcggcggt ccaggcccag
      121 ctcgcctcgc ccggcccgtt ctcgtcgacg acggccttcg cggggatagg gctatcgcgc
      181 tcggtcttcg gcgcggccga cggctacacg cccttcgtct cggccggcga ctacgcgtcg
      241 gtctggaacg gcgtggatct gctcggtacg gagctgccgt tccgcttcgg ctgggagctg
      301 gcctcgagcc tgtcggccgg gccggtcaag ctgagcttct cgctgccgtt ctacagcgac
      361 gcgtactaca acgtggactt ccgtgaccgc agcgagcaga tgaactggct ccagttcctc
      421 gaccaggacg acgacgagac gacggtatcg aagctatcgt ccttcacgga ttcgctcagc
      481 ctgtcggcgt cgctgccctc ggccagcctg ccgtcgtggc tgtcgaccgc gtcgctgacc
      541 aagttgaaca cctcgctggc ctggaattcg gtggtcaagc cgacgccgag cgactcgacc
      601 gaggccaagc tgttcgcggt cgacccgacg cgcgagttct tcgctccgta cgagtggacg
      661 gtgctcgact cgagcgccag cctgtcgggc tcgctgttca agtacccgag gaaggccggg
      721 aaggccgtcc ccacggcctc gggcccgacg gccgcggcct ccgcgacgga tgcccgaggc
      781 gacgaaggcg gcgaaggcga cgagcgcgtt ccggacgccg tcgaccgggc gctcgcggcc
      841 ctgccgagcc tcgaggcgcc ctgggccacc tccgcggcgc tggaaaaggc ggagaaggcg
      901 gagaaggcgg agaaggcggc cgcccccggc ttctccgcgc cggccctggc ctcgcccgag
      961 acctacgctg accgtacgcc gctatccgcc tcgatgtcgt ggtcgctcag cccgaccttc
     1021 gcctggaagc ggcgcttcct gaccacggcc tggaccgagc cggagctcgt ggactggtcg
     1081 gcgctgtacg agacgaggac ctcgagggcg gctggctcgg tcaatctggt aggcgcggcc
     1141 tacgacggtc tgctcgccct gacgctcggc ctgtcggcgt cgagccaggc ccaggatagg
     1201 ccgagcatct cgtcggacgc ggcctacgcg agcgagtcgc tcaagacgac ctgggccaag
     1261 caggacgcgc agtaccgtaa cgacaagctg agcgcgcagc tcaagctgac gagctccccg
     1321 ttccaggacc tgtggctctg gtcgccgacc agcttcggct actccctctc gtcgacgctg
     1381 tacgagtacg cgttcaagga gatggacgcg acggccccga cggacgcctc gaaggccgtc
     1441 tacgagacgg ttacggccga ctggaccgac gagacggtca gcgcgcacgc gctgtcgctc
     1501 gaccttggcc tgcagccctg gggctataag caggtgctct cgctccaggc ggacctgccg
     1561 ccccgactcg gcggctatac gggcaggctc agcctcaagg cgccctgggc caccctgacg
     1621 gtcggctccg agtactcgat gccgtccgag gacgaggagt acgtctggtc gcccctgagc
     1681 tcgagcctga cgctcggcga ggctccctgg ccgaagctga ccgggagcta cgtctgggat
     1741 atcgaggacg agaagccgac cgcgctgtcg ctgcaggtag cctggaacgg gttcaccgcc
     1801 tcggcgagcg ccaaggaggc ggtcgcgtac cggttgagct acggcgccgg ctggtcgtcg
     1861 gtcggcgacc cgtcgttcat ggtcaactcg atgtcgatgg cctacaagac gagctggaag
     1921 ccgccggcgg cctggcgccg ccgtatccag tggacgatgg acgtcaacgc ggccgcgcag
     1981 cagagtttcc tgcgcttcac cgactcgtac ctgaacttcg tctacggcct gaccttcaag
     2041 gtgcacgagc tcctggacct gagcctgtcg tcgacgagca ggaatgcgtc gctgtggcgc
     2101 tattttcccg ccgcgttcga gatacccgag gagatagccc tcgatccggt caatccgctg
     2161 accgacctcc tggcgtcgtt caatttcttc gatggtacgg gcgaggcgcg cagggcgtcg
     2221 ctgttcaagc tcaaggcgct cgagctgacc gctacccacc acctgcacga ctgggacctg
     2281 gcgctcaagc tgacggccag cccggtgctc gagaacctcg agtacgtgtt caagaccgac
     2341 ttctcggtga cgctgtcatg gaattcggtg tcccagatca agtcctccta ttccaagaag
     2401 ggcgatacgg tctcctggga ctgacaggcg ggcccggctt ccttgatttt ttcccccggc
     2461 ggggttagga tcggacaccg tggttcccac agcggccgta ggcatcgccc tggacgacac
     2521 gaggctccta gccgacgtct gcggcgtcaa cgattacaac cttcgcgtgc tcgagcgcct
     2581 cttgggggtg cccgtgttcg ttcgcggcaa cgagatctcg ctcgagtcgc cgcgtccgga
     2641 cgtccgggag ctgttcgcgg ccgtgatcga acagctcctg tcgtcgatcg acgacggcct
     2701 cccggcctct cccgagctca tcaccgcgat ccacgccagc ctgacgcccg atggccaggc
     2761 ggccccctcc ggcgcgtcct tcaaggactc ggcgatccag gtgcccggcg ccttctccaa
     2821 ggtctaccca cgcacggcca accaggccct gtacctgcgc gggctctcga gccacgacat
     2881 aagcgtggcg gtcggtccgg ccggcacggg caagacctac ctggcggtgg cctgggccct
     2941 gcgcgaggtg ctgtccaagg cgcgcaggcg gctggtgctg acgcggccgg tggtggaggc
     3001 gggcgagagc ctcggcttcc tgcccggcga cctggggcag aagatcagcc cctacctgcg
     3061 gcccctgtac gacgcgatgg aggccctggt gccctacgac gtggtcaagc ggctcgagga
     3121 taaccatacc atagagatag ccccgctcgc gtacatgcgc ggccggagcc tcaataactg
     3181 cgtcgtcatc ctggacgagg cgcagaatac caccaaggaa cagatgaaga tgttcctgac
     3241 gaggctcggg gaggggtcgg tggccgtggt gaccggcgac gcgacccaga tagacctgcc
     3301 tcgtaaatcg gagtccgggc tcgtccatgc gctcaaggtg ctgtcggaca tcgacgacat
     3361 cttcgtgtgc cgtctcgacg gcagggacgt ggtcaggaat cccctggtca ggaagataat
     3421 cgacgcctat gagtcagaga tctgatagtt acggccggct cgaccgcctg cgcgccgcgt
     3481 tcagcgaccg cgccatggtc cgccgctacg gggcgatagc cgtcgcggcc ctcgtgtgcg
     3541 tcgccgccta tatcggcttc ggcgccgcct cgggcgcggc cggcaagggc ggcttcgagg
     3601 cgggcaaggt ggccgaccgc gacgtggtcg ccgagcgcga cgtggtctat atcgacgtca
     3661 gggcgaccaa gatacgcacc gaggccgagc tccggctcgt gccggcggtc ttctcgctcg
     3721 acgacgccgc cacccgggcg gccctggccg agctggaggc cttcaggctc ggcttcgcct
     3781 ccctggccgg cgagatagag ggccgcgagg agttggcgct ccggctgcgc ggccgcttcc
     3841 ccgatctggc cgccacggac ctgcccgagc ggctgaccaa ctatccggcc ccgtccaacg
     3901 ctataagcca gggccgcgcg gtgctgaccg ccctcctcgg cgctggcgtg ttcgcgatac
     3961 cggactcggg cctcgagcaa tacaatcccg agagcgtcga gctgcgccgc tggaccgacg
     4021 gtccgctggt ctacgagcag gtgccgctgg agtcggcgac gacgactcgt cggctcaagg
     4081 ccgccgccct cgatatcgcc aagtcgctca agctgtcggc cacgatgtcc gcgctcgcgg
     4141 ccgacctcgc cgtcgcgttc gccagggaga acgccttctt cgacgcggag cagagcctcc
     4201 ggcgcctcga gtcggcccga gcgcgggtgg agccggtcat gcgcaagctc gccaagggcg
     4261 acaaggtgat acgggagggc ttcatcgtca cggccgagga cctcgagcgg ctcgccgccc
     4321 tgcgcggctc cgagatggag ttcgacgcgc tgtacgcgct ggctggcgtc ctgctgctcg
     4381 cggcgctcgg ggtagcgtcg gtcgccctgc tcggcagggg ctctaccggc atccgagtcg
     4441 aaggcgccag ctactggttc gtcctggccg tggctgtcgg ctatttcgtc gcgtcgagcg
     4501 ccctgtcggc cttcctgccg cccgttctcg cggcccatat cgacgccttc ctgccgacgg
     4561 ccctggtgtc gatgctggtc gccatcctga taggcgaacg cttcgcgctg atgttcacgc
     4621 tgatcatcgg ctccagcctc gtgctggtga ccggcctcga cgccttcgtg ccggcccagg
     4681 cgatagcctc cggactggcc ggtatcgtgc tcgtcaggcg cgccgacaag cgcatcgacc
     4741 tggtgcgcgc gggctcgcag ctggcggccg cgcgcttcgc cattggcttc gcgctggcgg
     4801 cgctggcgga ccgcggcgcg gtggcggccc tgtccacggc cttctggtcg gcggtcaacg
     4861 gcttcctctg cggcgtgctc gcgctgtcgt tcctgccggt gctcgagcat atgctcaacg
     4921 cgccgacggt cttcaggctc caggagctgt cggacctgaa cgcgccggcg ctcaagcgac
     4981 tcctgtcggt ggccccgggc acctacagcc actcggtgac ggtcgcccac ctggcggagt
     5041 cggcctgccg cgagataggc gccgacgcgc tcctggccag ggtcggcgcg tactaccacg
     5101 acatcggcaa gatcgagaag cccgagtact tcatcgagaa ccagtcgggc tacaacaagc
     5161 acgacgagat caacccgcgg ctgtcggcca cggtcctgcg cgggcacgtc aagttcggcc
     5221 tcgagcgggc ggacgcgctc aggttgcccg agtcggtcaa ggccatcatc gcggagcacc
     5281 acggcacctc gctcatcagc tacttcttcg cccaggcgca gaaggagtcg atggaggcga
     5341 gctccgagga atataggtac ccgggcccgc tgccctcgag caaggaggcg gccgtcgtga
     5401 tgctcgccga ctgcgtcgag gcggcctcga ggacgctcaa gcgcccgacc atcgggcggc
     5461 tcgagcagtt cgtcaaggag ctcgtgatgg accgcttcgg gaacggccag ctgtccatgt
     5521 cggcgctgac cttcagggac ctcgagctga tcatgaactc gttcgtgcgg gtgctgtccg
     5581 ggcacttcca ctcgaggata gagtatccga agaccaagga gcaggggtga acgccgtagc
     5641 cgtcgactat cgcgacatgg aggagccggc ctggctcgag cgcgccgcgg ccttcgccgt
     5701 ccgcgcgatg gacgggctcg ggctcgagga ctgggacctg tcgctctatt tctgcggcga
     5761 ggccttcatg gccggcctga acgccgagta ccgcggcaag gacggcccga ccgacgtgct
     5821 gtcgttctcg ct
//
LOCUS       DAOPHV010000094         5779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_18249_length_5779_cov_4.306080, whole genome shotgun sequence.
ACCESSION   DAOPHV010000094 DAOPHV010000000
VERSION     DAOPHV010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18249_length_5779_cov_4.306080"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PLQ29_03780"
     CDS             complement(<1..763)
                     /locus_tag="PLQ29_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HPG85797.1"
                     /translation="MDDSQNALARARAAVVSGLGSGLKTSLYLLRIMIPVSLAVALLK
                     WCGALDWAARFMAPAMGFVGLPGEASLVIVSSMLLNVYSAVAVIETLPLTMREITILA
                     VLCLSAHNLIIETAVMRKSGSSAIKMLSLRILGGVALAYALNLLLPGDASSVAASTAD
                     TARAALLPALGAWGLSTLRLVVKILLLVNGIMIAQRLMEAFRVLDFLSRLAAPLMRVF
                     GLSDNSSFLWIVINVVGYAYGAAIIMERVKGGAMKP"
     gene            complement(812..1729)
                     /locus_tag="PLQ29_03785"
     CDS             complement(812..1729)
                     /locus_tag="PLQ29_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPG85798.1"
                     /translation="MFSVTIWGDRGSMPVPGADTVLFGGNTACIEIRCGKRLIIIDAG
                     SGIRSLGDWLVRNELKKGPIDADVFLTHTHWDHIMGFPMFTPIYVPGTTLRIRGPVNF
                     EDETLEQIIGSQLSYRYWPVRQDELAATISYQSLKETQLDLGDGIVVRTKYLNHPVLC
                     LGYRVEYEGKAFATAYDHEPFSNLFPTDPADPDYDPVIAEEGQKAAREENERVEAFYR
                     GVDLLVHDTQYSAKEYKAGKMGWGHSSFEYAINAANRAGVGRLLLFHHDPNRTDTELQ
                     ELELFYQAKVAGKTRLRVDTSREGMTIQL"
     gene            complement(1746..2612)
                     /locus_tag="PLQ29_03790"
     CDS             complement(1746..2612)
                     /locus_tag="PLQ29_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPG85799.1"
                     /translation="MPGSANANRIKNYIISMPSLPTTVAKVMEICNNPKASPIDLDRV
                     ISLDPVLMGKVLKLINSAYYGLQNQVTSLVRAIIMLGVNTVKNLALSTAVLDKLADKS
                     GFRALNMEGFWRHSLSVGVVAKMIAKKKGVEAQRLDEFFAAGLLHDIGKIPLNNALSD
                     EYVRAMSLADRERIPLHLAEKRSIDLDHAESGAMIAEAWHLDGPIADAIRYHHNLELY
                     EGKSEEIVLAVAVADYYANREGIGFSGCLYPEKLPPEVFERLGVPKVWLEDIDAAVAA
                     EIEKASVFLKIR"
     gene            complement(2596..3273)
                     /locus_tag="PLQ29_03795"
     CDS             complement(2596..3273)
                     /locus_tag="PLQ29_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85800.1"
                     /translation="MDEELPPDEEIVELPLEDEGADESVEETPEPVLESEPEPEPNEE
                     PEDETEPDEESLEEDLECAPEYIDEPAFDDEADELITEEEDERPWDEPEAEPEPEPES
                     PPQPESAPKPAHEPRSELDEPRSELDEPRSEPEHPKDSIDPFGEEAAKLYVYLKDLTR
                     SLPDDKREAMEKSGVASKLDAIIDRVTMPSKAPPIPNEIMGVPISPRLAKLIEFMRRE
                     KRHAGKR"
     assembly_gap    3530..3629
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3630..5555)
                     /locus_tag="PLQ29_03800"
     CDS             complement(<3630..5555)
                     /locus_tag="PLQ29_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG85801.1"
                     /translation="MSTAKKKTDSLALAQNALRLRDFETAERYSRKHLLADPKSSEAR
                     MVLGTVYLRTDRASDALAAFSSAVEADASDLRALEGMAVSYLRLGKPLDALAALERAI
                     DLNPNDAELLFNLGNAHKALGNLRAAEMSFAKAIELDGSHVMAYNNLGNVYLSLGDAS
                     KAVQVYWRGLAIDPNYPSFHYNLGMAYEKLGKPSDAITEYAAAVKGRPGWIDAMTRYG
                     ECLARNAESKRAIDVFTQIIAIDPLNAQARNGIGLAYAEAGKPDEAVSWFRKALEADP
                     RFLLAAVNIQDVLEKTGRYAEAVGEIQALVDLMPDNDGLRVQLARLYKALGHLDLAMD
                     TITDVLRRSPNDIEALKLYGTLLQALGKPAKAKEVYKKIVKLDPKQASFRIELARLYR
                     EAGDPASAIQELDEYLVSRPKDQTARIELARAYADKGEHAHAAQILRELIESRPDDAE
                     AWLELSAAYQALGDKDRAVEAADRLVNIRGGRGRPDDMDGLNDSIAAYEKAVRAYSYD
                     MREAWERNIEVLRESLRKDAEGAGDIRGDEPDTIFDLGSADSIPVDEAEELIFLDERE
                     EPLDGVEEQDEEPIDEEPIDDDEPRVDALLEGEELYGDERPQGPQSEPTKEPEQAAPQ
                     PAPPSQQPQQPQQPFQQP"
     gene            complement(5552..>5779)
                     /locus_tag="PLQ29_03805"
     CDS             complement(5552..>5779)
                     /locus_tag="PLQ29_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85802.1"
                     /translation="VVYYGSAPGDYFGADAREGRSPIFVPGGSASGLTLTGLKNGSLY
                     FLAVAAYDGAHPPHIGDSSREISARPSRVSP"
BASE COUNT          849 a         1738 c         1930 g         1162 t
ORIGIN      
        1 gcggcttcat cgctccgccc tttacccgct ccatgatgat cgcggccccg taggcgtagc
       61 cgacgacgtt aatgacgatc cacaggaagc tggagttatc cgagaggccg aagacgcgca
      121 tgagcggggc ggccagcctc gaaaggaagt cgagcacgcg gaacgcttcc atgagccgct
      181 gcgctatcat gatgccattg acgagcagca ggatcttgac gaccagcctg agcgtcgaca
      241 ggccccacgc gccgagagcc ggaaggagcg cggcccgcgc cgtatcggcc gtcgacgcgg
      301 cgacggagga tgcgtctccc ggtagcagca gattgagcgc gtaggcgagc gcgacgccgc
      361 cgagtatcct gagtgacagc atcttgatcg ccgaggagcc ggacttccgc atcacggcgg
      421 tctcgatgat caggttgtgg gcggacaggc acagcacggc cagaatcgtt atctcacgca
      481 tcgtcagcgg tagcgtctcg atcaccgcca cggcgctgta cacgttgagc aacatcgagc
      541 tgacgatcac gagcgaggcc tcgccgggca ggccgacgaa gcccatggcc ggagccatga
      601 agcgggcggc ccagtccagc gcgccgcacc acttgagtag cgctacggcc aggctgaccg
      661 gtatcatgat gcgtagcagg tataacgagg tcttgagccc cgagcccagt ccgcttacga
      721 cggcggcgcg cgcccgggcg agcgcgttct gagagtcatc catcgggccg gattctcctc
      781 gcataggccc ctcctgtcaa ggtcgggcgg gctatagctg tatcgtcatg ccctctcggc
      841 tcgtatccac ccgtagccgc gtcttgcctg cgaccttggc ctggtagaac agctcaagct
      901 cctgcagctc ggtgtcagtg cggttcgggt cgtggtggaa gagcaggagc cgcccgacgc
      961 cggcgcggtt ggcggcgttg atggcgtact cgaacgacga atggccccaa cccatcttgc
     1021 cggccttgta ctccttggcg ctgtactgag tgtcgtgcac gagcagatcg acgccgcggt
     1081 agaaggcctc gacgcgctcg ttctcctcac gtgcggcctt ttggccttcc tccgctataa
     1141 ccgggtcgta gtcaggatcg gccggatcgg tggggaacag gttcgagaat ggctcgtggt
     1201 cgtaggccgt ggcgaaggcc ttgccctcgt attcgacgcg gtagccgagg cagagcactg
     1261 gatggttcag gtattttgtc ctgacgacga tgccatcgcc gaggtcgagt tgcgtctctt
     1321 tgagactctg gtagctgatc gtggctgcga gctcgtcctg cctgaccggc cagtatcgat
     1381 acgacagctg ggagccgatg atctgctcga gtgtctcgtc ctcgaaattg accggaccgc
     1441 ggatgcgcaa cgtggtgccg ggcacgtaga tcggcgtgaa catcgggaaa cccatgatat
     1501 ggtcccagtg ggtatgcgtc aggaagacgt cggcgtcgat agggcccttt ttgagctcgt
     1561 tgcggaccag ccagtcgccg aggctcctga tgcccgagcc ggcgtcgatg atgatgagcc
     1621 gcttcccgca ccgtatctcg atacaggcgg tattgccgcc gaacagcacc gtgtcggcgc
     1681 caggtaccgg catcgagccg cggtcgcccc agatcgtgac gctgaacata gtgggcctcc
     1741 agcctttatc taatcttcag gaagaccgag gccttctcga tctcggccgc caccgcggcg
     1801 tcgatgtcct cgagccagac cttcggcacg ccgagccgct cgaagacctc gggtggcagc
     1861 ttctcggggt agaggcagcc ggagaagccg atgccctcgc ggttggcgta gtagtcggcg
     1921 accgcgacgg ccaggacgat ttcctcgctc ttgccctcgt acagctccaa gttgtgatgg
     1981 taccgtatcg catccgcgat ggggccgtcc aggtgccagg cttcggctat catcgcgccg
     2041 gattcggcgt ggtcgagatc gatgctccgc ttctcggcca ggtggagcgg gatccgctcg
     2101 cggtcggcca ggctcatggc tcgcacatac tcgtcggaca gcgcgttatt gagcggtatc
     2161 ttgccgatgt cgtgaagcag gccggctgcg aagaactcgt cgaggcgctg agcctcgacg
     2221 cccttcttct ttgcaatcat cttggctacc acgccgacgg acagggagtg gcgccagaag
     2281 ccctccatgt tgagcgcccg gaagccggac ttgtcggcta gcttgtcgag gaccgccgtg
     2341 ctgagcgcca ggttcttgac cgtgttcacg ccgagcatga tgatggccct gaccaggctg
     2401 gtcacttggt tctggaggcc gtagtaggcc gagttgatca gcttgagcac cttgcccatc
     2461 aaaacagggt cgagggagat gacccggtcc agatcgatcg ggctggcctt cgggttgttg
     2521 catatttcca tgaccttggc gacggtcgtc ggcaggctgg gcatcgagat tatgtagttc
     2581 ttgatcctat tggcgttagc gcttcccggc atgtctcttc tcccggcgca tgaactcgat
     2641 cagcttggct agcctcggcg aaatgggtac gcccataatc tcgttcggga tgggcggcgc
     2701 cttggacggc atggtgacgc ggtcgatgat ggcgtcgagc ttgctggcca cgccgctctt
     2761 ctccatggcc tcgcgcttgt cgtccggcaa ggatcgtgtc agatccttca ggtagacgta
     2821 cagcttggcc gcttcttcgc cgaatgggtc gatcgagtcc tttggatgtt caggttcgct
     2881 tcgcggctca tcgagctcgc ttcgcggctc atcgagctcg cttcgcggct cgtgcgccgg
     2941 tttcggagcc gattccggct gtggcggcga ctctggttcc ggttccggtt ccgcctctgg
     3001 ttcatcccag gggcgctcgt cttcctcttc ggtgatcagc tcgtcggctt cgtcgtcgaa
     3061 ggctggttcg tctatgtact ccggagcgca ttcgaggtct tcttcgagcg actcctcgtc
     3121 gggctcggtc tcgtcttcag gctcttcatt aggctctggt tccggctctg attcaagtac
     3181 tggttccggc gtctcctcga ccgattcgtc ggcgccttcg tcctcgagcg ggagctcgac
     3241 gatctcttca tcgggcggaa gttcctcgtc caacggcaga tcttcttcag gctgagacaa
     3301 gtctggcggg tcgaggctcg gaggcttttg ccgttccggc gctcgaggcg gcggatacga
     3361 ggggaaaggc gggaactgcg ggtacggcgg atactgaggc ggcgcgagcg gtggcgcgta
     3421 ggacggctgt tggaaggccg gttggggcac ctggggctgc tggtacggct gctggggcga
     3481 ttggggttgc tggaacggtt gttggggctg ctggggctgc tgagacggcn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ggttgctgga acggttgttg gggctgctgg
     3661 ggctgctgag acggcggcgc gggctggggc gcggcctgct cgggttcctt ggtcggctcc
     3721 gactgcggac cctgcggccg ctcgtcgccg tacagctcct cgccctcgag cagcgcgtcc
     3781 acgcgcggct cgtcgtcgtc gatcggctcc tcgtcgatcg gttcctcgtc ctgttcctcg
     3841 acgccgtcga gcggttcctc tcgctcgtcg aggaagatca gctcctccgc ctcgtcgacc
     3901 ggtatggaat ccgcgctacc taggtcgaag atcgtatcgg gctcgtcgcc gcgtatatcg
     3961 ccggctcctt cggcgtcctt gcgcagcgac tctcgcagca cctcgatatt tcgttcccag
     4021 gcctcgcgca tatcgtagga gtaggcgcgt accgccttct cgtaggcggc gatcgagtcg
     4081 ttgagcccgt ccatatcgtc gggccggccc ctgccgcccc tgatgttgac gaggcggtcg
     4141 gcggcctcga cagccctgtc cttgtcgccg agggcctggt aggcggcgga cagttcgagc
     4201 caggcctcgg cgtcgtcggg cctcgactcg atcagctccc tgagtatctg ggcagcgtgg
     4261 gcgtgctcgc ccttgtcggc gtaggctctg gccagctcga tacgggcggt ctggtccttg
     4321 ggccggctga cgaggtattc gtccagctcc tggatggcgg aagccggatc gcccgcctcc
     4381 cggtacagcc tggccagctc gatcctgaac gacgcctgct tcgggtcgag cttgacgatt
     4441 ttcttgtaga cttccttggc cttggcaggc ttgccgagtg cctggagcag ggtgccatag
     4501 agtttgagcg cttcgatgtc gttgggcgac cggcgaagca cgtcggtgat cgtatccatg
     4561 gccaggtcga gatgcccgag cgctttgtac aggcgcgcca gctggacgcg aagtccgtcg
     4621 ttatcgggca tcaggtcgac gagggcctgg atttccccta ccgcctcggc gtagcggccg
     4681 gtcttctcga gcacgtcctg gatgttcacc gcggcgagta ggaagcgggg atcggcctcg
     4741 agggccttgc ggaaccagga gacggcctcg tccggcttgc cggcctcggc gtaggcgagc
     4801 ccgatgccgt tcctggcctg ggcgttgagc gggtctatgg cgatgatctg ggtaaacacg
     4861 tcgatggcgc gcttcgactc ggcgttcctg gccaggcact cgccgtatcg cgtcatcgcg
     4921 tcgatccagc cgggtcgccc cttgacggcg gccgcgtatt cggtgatcgc gtcggacggc
     4981 ttccccagct tctcgtaggc catgccgagg ttgtagtgga acgacgggta gttcgggtcg
     5041 atcgccaggc cgcgccagta gacctggacg gccttggagg cgtccccgag cgataggtag
     5101 acgttgccga ggttgttgta ggccatcacg tggctgccgt ccagttcgat ggccttggcg
     5161 aacgacatct cggcggcgcg gaggttgccg agggccttgt gggcgttacc gaggttgaac
     5221 agtagctcgg cgtcgttcgg gttcaggtct atggcccgct cgagcgcggc cagggcgtcc
     5281 aggggcttgc cgaggcgcag gtacgacacg gccatgccct cgagcgcgcg taggtcggag
     5341 gcgtcggcct cgaccgcgct ggagaacgcg gcgagcgcgt cgctcgcccg gtcggtgcgt
     5401 aggtacaccg tgccgagcac catgcgggcc tccgaggatt tcgggtcggc gagtaggtgc
     5461 ttgcgcgagt atcgttccgc cgtctcgaag tcgcgtagcc tgagcgcgtt ctgggcgagg
     5521 gcgaggctgt cggttttttt cttcgctgtg ctcatggcga gaccctcgat ggccgcgcgc
     5581 tgatctcgcg cgatgaatcc ccgatatggg gaggatgagc gccatcgtac gcggcgacgg
     5641 ccaaaaaata tagcgacccg ttcttgaggc cggtcagcgt caggcccgaa gcgctaccgc
     5701 cgggcacaaa gatcgggctg cgtccctcgc gggcgtcagc gccgaagtag tcgcctggcg
     5761 cggagccata gtataccac
//
LOCUS       DAOPHV010000095         5779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_18252_length_5779_cov_3.882075, whole genome shotgun sequence.
ACCESSION   DAOPHV010000095 DAOPHV010000000
VERSION     DAOPHV010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18252_length_5779_cov_3.882075"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PLQ29_03810"
     CDS             complement(<1..401)
                     /locus_tag="PLQ29_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HPG85803.1"
                     /translation="MSDNRRVLKETFTTNLSDYTVAIAAVAVVLMIIIPVPTVLLDVL
                     MAFNVALSLMILLLVLSTRRAVDFSVFPTMLLVSTIFGLALNISSTRLILTKGQDFDG
                     RIVRAFSTFVIGAGGTEGLVVGFIIFIVIIAV"
     gene            complement(445..1596)
                     /gene="flhB"
                     /locus_tag="PLQ29_03815"
     CDS             complement(445..1596)
                     /gene="flhB"
                     /locus_tag="PLQ29_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HPG85804.1"
                     /translation="MSDGRRAPDDEILAIHLQWFAPEDEGKTEEPTEYRLKKEREEGR
                     VPKSPDLVAAIGLLLASVALAIIGPWIFYNLREMLRWFLEASIEIDPTTEAGPIAARF
                     FSYFAKLILPIASVSMIAGVFGNVLQSGFLFTTKPLKPDFKKVVPKFGQYFKRTLLSG
                     EALFNLAKSIFKIVVIGVVAFLNIRGELPRLTRLFSSTVWNSSVYLAGIVIRIVIEAA
                     ILMLALAIPDYIFQRAQFKKRLRMTKQEVKEERKMFEGDPMVKGRLKDRMRDIMSRNM
                     AVNVPKADVVVTNPTHFAIAVEFDPMTMAVPTVTAKGADEMAFRIRSLAKDAGVPIVE
                     NRPLARALYAEADVGDAIPEAYYEAISKILAHVAKIDRRVAAKYAAMGR"
     gene            complement(1593..2405)
                     /gene="fliR"
                     /locus_tag="PLQ29_03820"
     CDS             complement(1593..2405)
                     /gene="fliR"
                     /locus_tag="PLQ29_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HPG85805.1"
                     /translation="MLNAIVADADLFFIVAVRVLAMVETAPLLSGEGIPQVAKVGLAG
                     FAAAAVFPWVKEAGYAMPVGAGSYVAVVAGEAMIGVIIGFFLTMVFNAFTTAGQFFSL
                     QMGFGASETYDPLAQIEIPVVGQFLNLIAMFVFVSSNGFQRLFLVGVRGSFQAIRAAD
                     LLARRDDFMAYVVGSLGALFQNALILAFPIFGTLMLISVTTGLLSKAAPQINLLTEGF
                     PISILVGFFLMVVAMPYMIEAFSSLVDIGFENVAAILQGVAAGRVVSPGGGL"
     gene            complement(2410..2679)
                     /gene="fliQ"
                     /locus_tag="PLQ29_03825"
     CDS             complement(2410..2679)
                     /gene="fliQ"
                     /locus_tag="PLQ29_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HPG85806.1"
                     /translation="MNTAQAAALMSGAVLEIVTLAAPVLLTALVVGLIVSILQATTSI
                     QEQTLTFVPKIASIMLVLALLGGWMFTHLAEYTKRLFDLIPTLAG"
     gene            complement(2676..3587)
                     /gene="fliP"
                     /locus_tag="PLQ29_03830"
     CDS             complement(2676..3587)
                     /gene="fliP"
                     /locus_tag="PLQ29_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520315.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HPG85807.1"
                     /translation="MRRAIAVLILLLSAAGAWAQDAAAYPAVPADTARSVPIPSAGTT
                     RATTGAGSGTSSFPSGAASATTAIPRNLPVVPFVDLSVRSPETGGEVAFSLQLLLLLT
                     VLSLAPSLIILVTSFLRVSIVLDFVKRALALQQVPPGQVLMGIALFLTLFIMWPTFDE
                     VYTQSLKPLADGQIGVEEAYRAAEKPFRLFMYSQMRGDAENIRLFMSMRGLPKPESLA
                     DVPTYVLVPAFILSELTIAFKIGILLYIPFIIIDMVVASVLMSMGMIMLPPVMISTPF
                     KLILFVLVDGWNLMARQLIESFMRNAL"
     gene            complement(3611..4207)
                     /locus_tag="PLQ29_03835"
     CDS             complement(3611..4207)
                     /locus_tag="PLQ29_03835"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HPG85808.1"
                     /translation="MDRCIRIACISLALALTAVAAFAQGAPDRASPDQAAAVDETSLV
                     FDATAVPGAETPADSSIFPYVIRMILVLALILGCIYGLYALIKRSGKPGVATDSYLRV
                     LASTPLAVGRNLHVVALGERAWLVGSTDASVSLIAEVADKELIDALALRAAEAPQAPR
                     KDFGAMLMEMLGRKNAKAGFAPRSGDFLSGQRDRLRKF"
     gene            complement(4317..5414)
                     /locus_tag="PLQ29_03840"
     CDS             complement(4317..5414)
                     /locus_tag="PLQ29_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HPG85809.1"
                     /translation="MTDGRLDSPDRPALNLIERLSNAAGAPGFEDDVLAVLRDAAGPA
                     SAVTEDSVRNLYLRRDGFGKNGPVVMLDAHTDEVAFMVQAIKPNGTLRFVPLGAWVPN
                     TVPAHRVLVRDADGRYIPGVIASKPPHFMTEAEKAKPLDIEDLAIDIGATSKAEAEDV
                     FRVRIGEPVVPDVDFRYDAARDLMTGKAFDCRLGCAAVIETMRRASGLPLRVEVVGAL
                     ASQEEVGMRGAGVTARAVNPAAAIVFEGCPADDTFADDYMVQTALKRGPMLRFIDRMM
                     ITNPRFQRFALDLGAELGIPVQASVRSGGATNGGPIHLSGPGVPVIVIGLPVRYIHTH
                     YGIASYADFENSVRLALAVVSRLDEAVIRGF"
     gene            complement(5434..>5779)
                     /gene="fliN"
                     /locus_tag="PLQ29_03845"
     CDS             complement(5434..>5779)
                     /gene="fliN"
                     /locus_tag="PLQ29_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HPG85810.1"
                     /translation="MYQSPIMGQPNVQPVQFANLGLGNPGQESGNIGLIMDVFMELTV
                     ELGRTKKLIKDILGIGEGTIIELDKLAGEPVDILVNHKLIAKGEVVVIDENFGVRVTE
                     IVSPMERMADLS"
BASE COUNT         1099 a         1792 c         1915 g          973 t
ORIGIN      
        1 acggcaatga tcacgatgaa gatgatgaaa ccgacgacga ggccctcggt accgccggcg
       61 ccgatgacga aggtgctgaa cgcccgtacg atgcggccgt cgaaatcctg gcccttcgtc
      121 aggatcagcc tggtcgacga tatgttgaga gccaagccga agatggtcga gacgagcaac
      181 atggtcggga agaccgagaa gtcgacggcc cggcgagtcg acaacacgag cagcaggatc
      241 atcagcgaca gcgccacgtt gaaggccatc agcacgtcga ggaggaccgt cggtaccggg
      301 atgatgatca tcagcacgac ggctaccgcg gctatcgcca cggtatagtc cgagaggttg
      361 gtggtgaagg tttccttgag tactcgcctg ttatccgaca tgtcgccttc ccatgctatc
      421 ctaaaaagcg cgtttcaaca atgatcacct gcccatcgcg gcgtacttgg cggcgaccct
      481 ccggtcgatc ttggcaacgt gggcgagtat cttcgagatg gcctcgtagt aggcctcggg
      541 gatcgcgtcc ccgacgtcgg cctcggcgta gagcgcccgc gcgagcggtc tattctccac
      601 gatcggtacg ccggcgtcct tggccagcga gcgtatgcgg aaggccatct cgtcggcgcc
      661 cttggccgta accgtaggca cagccatcgt catcgggtcg aactcgacgg ctatggcgaa
      721 gtgagtcggg ttggtcacga ccacgtcggc cttcggcacg ttgaccgcca tgttcctgga
      781 catgatatcg cgcatcctgt ccttgagcct gcccttcacc atcgggtcgc cctcgaacat
      841 cttgcgctcc tccttgacct cctgcttggt catcctgagg cgcttcttga actgggcgcg
      901 ctggaagatg tagtctggaa tcgccagggc cagcatcagg atcgcggcct cgatgacgat
      961 gcgtatgacg atgcccgcca ggtagacgct cgaattccat accgtcgagg agaacaacct
     1021 cgtcaggcgc ggtagctcgc cgcgtatgtt gaggaacgcg acgacgccta tcacgacgat
     1081 cttgaagatc gacttggcca gattgaacag cgcctcgccg gacaacagcg tccgcttgaa
     1141 gtactgaccg aacttgggca cgaccttctt gaagtcaggt ttgagcggct tggtcgtgaa
     1201 caggaagccc gactgcagca cgttgccgaa cacgccggcg atcatcgaca cggacgctat
     1261 cggcaggatg agcttggcga agtacgagaa gaaccgggcc gcgatgggac cggcctcggt
     1321 cgtggggtcg atctctatgg acgcttcgag gaaccagcgg agcatctccc tgaggttata
     1381 gaagatccac gggccgatga tggcgagggc gaccgaggcc aggagaagcc ctatcgcggc
     1441 cacgaggtcg gggctcttcg gtacccggcc ctcctctcgc tccttcttaa ggcgatactc
     1501 ggtcggctcc tcggtcttgc cctcgtcctc gggagcgaac cactggaggt gtatcgcgag
     1561 gatctcgtcg tcgggagccc ggcggccgtc gctcacaggc cgcctcccgg cgatacgacg
     1621 cggccagccg cgacgccttg caggatggca gccacgttct cgaagccgat gtcgacgagc
     1681 gacgagaagg cctcgatcat gtacggcatc gccaccacca tcaggaagaa cccgacgagt
     1741 atcgatatgg ggaagccctc ggtcaggagg ttgatctggg gcgcagcctt ggacaggaga
     1801 ccggtcgtca ccgagatgag catcagcgta ccgaagatcg ggaaggccag gatcagcgcg
     1861 ttctggaaca gcgcgccgag agagccgacg acgtaggcca tgaagtcgtc gcggcgagcc
     1921 aacagatcgg cggcccggat ggcctggaag ctgcccctga cgccgacgag gaacagtcgc
     1981 tggaagccgt tcgacgagac gaatacgaac atggctatca ggttgaggaa ctggccgacc
     2041 acgggtatct cgatctgagc caggggatcg taggtctcgg aggcgccgaa gcccatctgc
     2101 agggagaaga actggccggc ggtcgtaaac gcgttgaaga ccatagtcag gaagaagccg
     2161 atgatcacgc ctatcatcgc ctcgcccgcg acgacggcga cgtacgagcc ggcgccgacg
     2221 ggcatggcgt agcccgcttc cttgacccag gggaagacgg ccgcggcggc gaagccggcc
     2281 aggccgacct tggctacctg cggtatgccc tcgccggaca gcaggggcgc cgtctcgacc
     2341 atggccagca ctcgtacggc cacgatgaag aacaagtcgg cgtcggcgac gatcgcgttc
     2401 agcacgcggc tagcccgcca gcgtcggtat gaggtcgaag agccgcttcg tgtactcggc
     2461 caggtgcgta aacatccagc cgccgagcag ggcgagcacc agcatgatcg acgcgatctt
     2521 gggcacgaag gtgagcgtct gctcctggat gctcgtcgtc gcctgtagga tgctgacgat
     2581 gagaccgacg acgagagccg tcaagagcac cggcgccgcc aacgtgacga tctcgagtac
     2641 cgccccgctc atcagggccg cggcctgggc ggtgttcata gggcattcct catgaacgac
     2701 tcgatgagct gcctggccat cagattccag ccgtcgacga gcacgaacag gatcagcttg
     2761 aacggcgtcg atatcatgac cggcggtagc atgatcatgc ccatcgacat gagcacggag
     2821 gctaccacca tgtcgatgat gatgaacgga atatagagga ggatgcctat cttaaaggcg
     2881 atggtcagct cgctgaggat gaaggccggc acgagcacgt aggtcggcac atcggcgagc
     2941 gactccggct tgggcaggcc gcgcatgctc atgaacagcc tgatgttctc tgcgtcgccc
     3001 ctcatctggg agtacatgaa cagcctgaac ggcttctccg cggctcggta ggcctcctcg
     3061 acgccgatct gaccatcggc gaggggtttc agcgactggg tatagacctc gtcgaaggtc
     3121 ggccacatga tgaacagggt gaggaacaag gcgatgccca tcagcacctg ccccggcggc
     3181 acctgctgta gcgccagggc ccgcttcacg aagtcgagca cgatggacac gcggaggaaa
     3241 ctcgtcacga ggatgataag gctcggcgcc agggatagca ccgtcaggag caggagcagc
     3301 tgcaggctga aggcgacttc gccgccggtc tcgggcgagc gtacggacag gtcgacgaag
     3361 ggcacgaccg gcaggttccg cggtatggcc gtggtggcgc tagcggctcc cgacgggaac
     3421 gaggaggttc ccgagcctgc acccgtcgtg gcccgcgtcg ttccggcgct cggtatgggc
     3481 acgctcctgg cggtgtcggc cggcacggcc ggataggccg ccgcgtcctg ggcccaggcc
     3541 ccggcggccg atagcagcag tatgagtacg gcgatggctc ttcgcacgcg ttcctcccgc
     3601 ccgtcccagg ctagaatttc ctgagccggt cgcgctgacc gctcaggaag tcgccggaac
     3661 gcggcgcgaa cccggccttg gcgttcttgc ggccgagcat ctccatgagc atcgcgccga
     3721 aatccttacg aggcgcctga ggcgcctctg ccgccctcaa cgccagcgcg tctatcagct
     3781 ccttatccgc tacctccgcg atcagcgata ccgaggcatc ggtcgagccg acgagccagg
     3841 cccgttcgcc gagcgcgacg acgtggagat tccttcctac agcgagcgga gtcgaggcga
     3901 gcacgcggag gtacgagtcg gtggcgacgc cgggcttgcc tgaacgcttg atcagtgcgt
     3961 acagtccgta gatgcatccg aggatcagcg ccagtacgag gatcatgcgt atgacgtacg
     4021 ggaagattga cgagtcggcg ggcgtttcgg cgccgggtac tgcagtggcg tcgaagacca
     4081 ggctcgtctc gtcgacggcg gctgcctggt cgggagacgc tcgatcgggc gccccttgcg
     4141 cgaacgcggc gacggccgtc agggcgagcg cgagcgatat gcacgcgatg cggatacacc
     4201 tgtccatgtc ccctccgctg gcattgtaca gctaagcggg gagaatggca acaagtcttt
     4261 gagcaataag gaatacctta gccagcctaa aggaggggcg aaggcccggc cggggcctag
     4321 aaacctcgta tcaccgcttc gtcgaggcgc gataccacag ccagggccag gcgtaccgag
     4381 ttctcgaaat cggcgtacga ggcgatgccg tagtgcgtat ggatgtagcg gaccggtagc
     4441 ccaatgacga tgaccgggac gcccgggccg gacaggtgaa tcggcccgcc gttggtcgcg
     4501 ccgcctgaac ggaccgacgc ctgtaccgga atgccgagct cggcccctag gtcgagggcg
     4561 aagcgctgga aacgggggtt ggtgatcatc atacggtcta tgaaacgcaa catcggaccg
     4621 cgtttcagcg ccgtctggac catgtagtcg tcggcgaagg tatcgtcagc ggggcagcct
     4681 tcgaacacga tggcagccgc agggttcacc gcccgcgcgg tcactccggc gccgcgcatg
     4741 ccgacctcct cctgcgaggc cagcgcgccg accacctcga cgcgtagcgg cagcccgctc
     4801 gcccgcctca tcgtctcgat gacggcggcg cagccgagcc ggcagtcgaa ggccttgccc
     4861 gtcatcaggt cgcgagcggc gtcataccgg aagtcaacgt cgggcaccac cggctcgccg
     4921 atcctgacgc ggaagacgtc ctcggcctcc gccttgctcg tcgcccctat gtcgatcgcc
     4981 aggtcctcaa tgtcgagcgg cttggctttc tcggcctcgg tcatgaagtg aggcggcttg
     5041 gaggcgatga cgccgggtat gtagcggccg tcggcgtcgc gcacgagcac gcggtgggcg
     5101 gggacagtgt tcggtaccca agcgccgagc ggcacgaagc gcagcgtacc gttcggcttg
     5161 atggcctgga ccatgaaggc cacctcgtcg gtgtgcgcgt cgagcatcac gaccgggccg
     5221 ttcttgccga acccgtcgcg gcgaagatag aggttgcgta cgctgtcctc ggttacggcg
     5281 ctcgcggggc cagcggcgtc ccggagtacg gccaggacgt cgtcctcgaa gcccggagcg
     5341 ccggccgcgt tcgacaggcg ctctatgagg ttgagcgccg ggcggtcggg cgagtcgagc
     5401 cgtccgtcgg tcatggaggt cgggtccccg ggtttacgac aggtccgcca tgcgctccat
     5461 cggagaaacg atctccgtga cgcgcacgcc gaagttctcg tcgatgacga cgacctcgcc
     5521 cttggctatc agcttgtggt tgacgagtat gtcgaccggc tcgccggcca gcttatcgag
     5581 ctcgatgatc gtgccctcgc ctatgcccag gatgtccttg atcagcttct tggtgcgccc
     5641 gagctccacg gtcagctcca tgaagacgtc catgatcagg ccgatattgc cggattcctg
     5701 cccgggattg ccgagaccca ggttggcgaa ctgtacgggc tgcacgttcg gctggcccat
     5761 gataggcgat tggtacata
//
LOCUS       DAOPHV010000096         5777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_18262_length_5777_cov_4.440930, whole genome shotgun sequence.
ACCESSION   DAOPHV010000096 DAOPHV010000000
VERSION     DAOPHV010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18262_length_5777_cov_4.440930"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1568
                     /locus_tag="PLQ29_03850"
     CDS             <1..1568
                     /locus_tag="PLQ29_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85811.1"
                     /translation="EDNAAYPRPVTINYLDYNPESSWETYGTAFPTGSGSVTAAIATE
                     LPSANGTRYVYAKLIDEAGNATGYMYDTIILDTVAPTVSFSINGGATTTPSLSFFLTL
                     TASDNLSTDSQLEVRDRNIYGSGWMPYRAYATSMLSDFQWPASAGNKTTYVGVRDAAG
                     NEAQASDSITLEVPVPTYATKGTYSGGYSYVYYEPVTDPAGGSYTTRYYVYSTTDPDA
                     SPNSGDPVTYRYNTTSASSSYAPIPNGELHYFFVRAYDSDTGGYGPYSAASVLGFSSN
                     VTVVYDDDEPADVTRAEALKAILEDDQGITGLANIYGAMPTWSVTLLPEDLISATYST
                     EARIYGDPIIVTHGSSFTTASTNDGRVRNIAASSKGTIAMGTGGAYFIYRVDQNWSAW
                     ALTGTQPSDIDSGNQMGLNAIRSSKTVASSASEDIWYTPLYYGVLYNSYRESSVAVNI
                     FSADTERRGIYISSGANPTGGYIYAGDAYSGTYFPVVRQGEYCYYGYYEIPDAPATGQ
                     VFLINLVSRMASF"
     gene            1674..2624
                     /locus_tag="PLQ29_03855"
     CDS             1674..2624
                     /locus_tag="PLQ29_03855"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPG85812.1"
                     /translation="MHVHNPILKASLALAVATLFAVAASSCSPKRGAEPLRLGIMPDA
                     DSLPFIVAEAEGLFEKRGVEVELVSFANAQERDAAIQAGRLDGAVSDVLAAAFLAAGG
                     FDTRITSATDGRYGIAAAPGSGLGSAAELAGKRVGLSTNTIIQYSVDAIVGASGLAPD
                     AYTAVAIPKMPVRMEMLLAGQVDAAGLPEPFLTTAVERGATLVGTTEQFHIDAAVVVF
                     SEAVLNARLKDVLRLYEAYADASGRVNAAPESYRAFLVEKARFPEDAAGAYRFVTYRK
                     PTMPPLGQVEAAVSWLRERNLLSKPVDPSALLDDRAVVAW"
     gene            2618..3484
                     /locus_tag="PLQ29_03860"
     CDS             2618..3484
                     /locus_tag="PLQ29_03860"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG85813.1"
                     /translation="MVELRELTIDYPGGSSPGWTGRLGRPGPRVPAVAGVDAVFGSGA
                     ISAIVGPSGCGKSSLVGAMAGLLAPSAGEALIDGEPVRGVRPRTAVIFQDYGLLPWLT
                     VEGNAELPLAVRGIGRSERRRRVGPILHELGLSSFGGFYPSRLSGGMKQRVAIARALA
                     SEPDLLLMDEPFSSLDALTREAAQEALLEVRRSRPMTIIVVTHAIEEAAYLAEAVFVM
                     GGRNPGTIRARFDIPENAARVRFAPGAAGDFRSASRYLELCGAIRSALRGSGPTDDSA
                     SPGMAPIPEARR"
     gene            3508..4251
                     /locus_tag="PLQ29_03865"
     CDS             3508..4251
                     /locus_tag="PLQ29_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG85814.1"
                     /translation="MATRRAASIALAVAVIVAVWSAAAAALGKPFLPSPATTFAAFGR
                     LAATGSLGRHAAASASRVLWALAASFFPAAALGLAAGRSRRVDAVVAPVVYVLHPLPK
                     VAFLPVIMLVFGLGEASKVILVALIVFSQILVAARDAARRIPRPLVDSVRSLGASRLD
                     LAFRVVVPSTLPDLLTSLRVSLGTAVAVLFLAETFATETGLGYLIVDAWARVAYAEMY
                     AAIVALSLLGLGFFMAVDLAERLLCPWRS"
     gene            complement(4277..>5777)
                     /locus_tag="PLQ29_03870"
     CDS             complement(4277..>5777)
                     /locus_tag="PLQ29_03870"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPG85815.1"
                     /translation="IATAIALAWIVIDRASVRTHLEMAERNRALQVELARNAMEEGFE
                     RLLQENRVLAAYSFPEYARGLRGRASMEALLEAEGDAYPESLSYCYLTGPGRLELAWA
                     RPGAEETMEALVFASVAQWDGFLGMSDPVVLSSRSNRPEPYFMALFPVHVGGDMVGIL
                     ATAIGLGRSIDTYLSPLAEGEGRRSFLMFGAGRVLWASDDRNPSLFELDQGSLMTSRS
                     FALGDGEFTIIADESRASLLSELEAIDRPRFLVGAMGLLTMVAALFFANRLYLERRGR
                     QALASEERRLSARVAAGERELRALLDDRELLLRELHHRVKNNFQYLDSLIELQKGGAS
                     ADAAAALSNIQARIAALAAAYLINADRPESLRVDARDYLTALAGKVVDGAGVELVIEA
                     EDIPVSLDSAVPLGLLFHELLTNAVRHGYAGKRGYAGGHGDRVAVRFYRDGGTAVLEV
                     RDFGTGLEAGTADGLGLTIVRALVAQLNGGFSLENREPGVIAEARFPLA"
BASE COUNT          935 a         2050 c         1867 g          925 t
ORIGIN      
        1 cggaagacaa cgcggcgtac ccgaggccgg tcacgatcaa ctacctggac tacaaccccg
       61 agtcgagctg ggagacgtac ggcacggcct tcccgaccgg ttccggctcg gtcaccgccg
      121 cgatcgccac cgagctcccg agcgccaacg gcacgaggta cgtctacgcc aagctgatcg
      181 acgaggccgg gaacgccacc ggctacatgt acgacaccat catcctcgac acggtggctc
      241 cgacggtttc attcagcatc aatggcggcg cgacgacgac gccgtccctg tcgttcttcc
      301 tgacccttac ggccagcgac aacctgtcga cggacagtca gctcgaggtc agggaccgga
      361 atatctacgg gagcggatgg atgccgtacc gggcctacgc cacctcgatg ctatcggact
      421 tccaatggcc cgcctcggcc gggaataaga cgacgtacgt cggcgtacgc gacgcggccg
      481 gtaacgaggc ccaggcgtcc gacagcatca ccctcgaggt accagtgccg acctacgcca
      541 cgaagggcac gtactccggc ggctactctt acgtctatta cgagcccgta accgaccccg
      601 ccggcggtag ctacaccacc aggtactacg tctactccac gaccgatccc gacgcgtccc
      661 cgaactcggg cgacccggtc acctatcggt ataacacgac gagcgcctcg agtagctacg
      721 cgccgatccc gaacggcgaa ctgcactact tcttcgtgcg cgcctacgat agcgataccg
      781 gaggctacgg cccgtacagc gccgcgtccg tcctgggctt ctcgtcgaac gtcacggtcg
      841 tctacgatga tgacgagccg gcggacgtga ctcgagcgga ggcgctcaag gcgatacttg
      901 aggacgacca gggcatcacg ggactggcca acatctacgg cgccatgccg acctggagcg
      961 tcacgctact accggaagac ctgatctctg cgacatactc gacggaagcg aggatctacg
     1021 gcgacccgat catcgtcacg cacggctctt ccttcacgac ggcctcgacc aacgacgggc
     1081 gcgtcaggaa catcgcggct tcgagcaagg gaaccatcgc gatgggtacg ggtggcgcct
     1141 acttcatcta ccgcgtcgac cagaactgga gcgcatgggc cctgaccggg acccagccgt
     1201 cggatataga ctcgggcaac cagatgggcc tcaacgccat tcgctcgtcc aagaccgtcg
     1261 cctcgtccgc ctcggaggac atctggtaca cgccgctata ctacggcgta ctctacaact
     1321 cgtaccggga gtcgtcggtg gcggtcaata tcttctcggc ggacacggag cgccgcggga
     1381 tctatatctc atccggagcc aacccgacag gcggctacat ctacgcgggc gacgcgtact
     1441 ccgggacgta cttcccggtc gtcaggcagg gcgagtactg ctactacggc tattacgaga
     1501 tacccgacgc gccagcgacc ggacaggtgt tcctgatcaa tctcgtctcg cggatggcca
     1561 gcttctaagg cggttattgc cttaggagcc gggctccggt agactctgag gctcgggcgc
     1621 ggcgcctgac aatcgatcgc ggtcgtccgg cgccacgccg acaggagcct ccgatgcatg
     1681 tacacaatcc cattctcaaa gcgtcgcttg ccttggccgt agcgaccctg ttcgccgtgg
     1741 ccgcctcgtc ctgttccccg aagcgagggg cggagccgct ccggctcggc atcatgccgg
     1801 acgccgactc attgcccttc atcgtcgccg aggccgaggg tctattcgag aagcgcggcg
     1861 tcgaggtgga actcgtcagt ttcgcgaacg cgcaggagcg cgacgccgcc atccaggccg
     1921 gccggctaga cggcgccgtc tcggacgtac tcgccgcggc ctttctcgcg gccggcggtt
     1981 ttgacacgag gatcacgagc gcgaccgacg gtcgctacgg catcgccgcg gcccccggtt
     2041 cgggcctggg ctcggcggcc gagttagccg gcaagcgcgt cgggctgtcc accaacacga
     2101 tcatccagta ctcggtcgac gccatcgtcg gagcctcagg cctcgccccg gacgcctata
     2161 cggccgtggc catacccaag atgccggtac gcatggagat gctgctcgcc ggccaagtgg
     2221 acgccgccgg cctacccgag cccttcctga ccacggccgt cgagcgcggc gccacgttgg
     2281 tcggcacgac cgaacagttc cacatcgacg cggcagtcgt cgtgttctcc gaggccgtgc
     2341 tgaacgccag gctcaaggac gtgctcaggc tctacgaggc ctacgccgac gcctcaggcc
     2401 gcgtcaacgc ggcgcccgag tcgtaccgcg ccttcctcgt cgagaaggcc cgcttccccg
     2461 aggatgccgc cggcgcgtac cgcttcgtga cctatcggaa gcctaccatg ccgccgctcg
     2521 gccaggtcga ggcggccgta tcgtggctca gggagcggaa tctgctctcc aaacccgtcg
     2581 atccatcagc cctgctcgac gacagggccg tcgtggcgtg gtagagctcc gtgagctgac
     2641 tatagactac ccgggcggct catcgcccgg atggaccggg cggctcggcc gccccggacc
     2701 ccgcgttccg gccgtcgcgg gcgtggacgc cgtcttcggc tcgggcgcga tctcggccat
     2761 cgtcggtccg tcgggctgcg gcaagtcgag cctggtaggc gcgatggccg gtctgctcgc
     2821 gccgagcgcc ggcgaggcgc tgatcgacgg cgagccggta cgcggcgtac ggccccgtac
     2881 cgcggtgatc ttccaggact acggcctcct cccctggctg acggtcgagg gcaacgccga
     2941 actgccgctg gccgtgcgcg gcatcggccg gtcggagcgg aggcgccggg taggccccat
     3001 actccatgag ctcggcctgt cttccttcgg gggcttctac ccgagccgcc tctcgggcgg
     3061 catgaagcag cgcgtcgcga tagcccgcgc cctggcctcc gagcccgacc tgctcctgat
     3121 ggacgagcct ttctcgagcc tcgacgcgct gacacgcgag gccgcccagg aagccctgct
     3181 cgaggtcagg cgctcgagac cgatgacgat catcgtcgtg acccatgcga tagaggaggc
     3241 ggcctatctg gccgaggcgg tcttcgtgat gggcggtcgc aacccgggaa ccatccgcgc
     3301 ccgtttcgac attcccgaga acgcggctcg ggtccgcttc gcgccgggcg ccgccgggga
     3361 cttcaggtcg gcctcgcgct acctggagct atgcggcgcg atccgctcgg cgctcagggg
     3421 ctccggcccg accgacgact cggcgagccc cggaatggcg ccgatcccgg aggcgcgaag
     3481 atgaggctcg gagggcggaa cggcgccatg gcgacgagaa gggccgcctc catcgcgctg
     3541 gccgtcgcgg tcatcgtcgc cgtctggtcg gcggccgccg ccgcgctcgg gaaacccttc
     3601 ctgccgagcc cggcgacgac cttcgcggcg ttcggccgcc tggccgccac tggctcgctc
     3661 ggtcggcatg ccgccgccag cgccagccgc gtactctggg ccctggccgc gagcttcttt
     3721 ccggcggcgg ccctcgggct ggcggccggc cgctcgcgcc gggtcgacgc cgtcgtcgct
     3781 ccagtcgtct acgtcctcca cccgctaccc aaggttgcct tcttgcccgt catcatgctg
     3841 gtcttcgggc tcggcgaggc ctcgaaggtc atcctcgtcg cgctgatcgt cttcagccag
     3901 atactcgtgg cggcccgcga cgcggccaga cgcataccgc ggccgctcgt cgactcggtc
     3961 cgctcactcg gcgcgtcccg gctcgacctg gcgttccggg tcgtcgttcc gtcgacgctg
     4021 cccgatctgc tgacctcgct acgagtcagc ctcggcacgg cggtcgcggt gctgttcctc
     4081 gctgagacct tcgctaccga gacggggctc ggctacctga tcgtcgacgc ctgggccagg
     4141 gtggcgtacg cggagatgta cgccgccatc gtcgcgctaa gcctcctggg gctcggcttc
     4201 ttcatggcgg tggacctggc cgagcggctg ctctgcccat ggcgctcgta gagatgcccg
     4261 tagaggcttt tccgggtcag gccaggggga atcgggcttc cgcgatgacg cccggctctc
     4321 gattctcgag gctgaaaccg ccattgagct gcgcgacgag ggcccggact atcgtcaggc
     4381 ccaggccgtc cgccgtaccg gcctcgaggc cggtaccgaa atccctgacc tcgagcaccg
     4441 ccgtcccccc gtctcggtag aatcggacag ctaccctgtc tccgtgcccc ccggcatagc
     4501 cgcgtttccc ggcgtaaccg tggcgtacgg cgttggtcag gagctcgtgg aacaggagac
     4561 cgagcggcac cgccgaatcg agagagacag gtatgtcctc ggcctctatg acgagctcga
     4621 cgcccgctcc atcgacgacc ttgcccgcca gggcggtcag gtagtcgcgg gcatcgaccc
     4681 ggagcgactc cgggcgatcg gcgttgatca ggtaggcggc ggcgagcgcg gctatccgag
     4741 cctggatatt cgatagcgcg gcggccgcat cggctgaggc gccgcccttc tgtagctcga
     4801 tgaggctatc caggtactgg aagttgttct tcacgcgatg atggagctcc cgtaagagca
     4861 gctcgcggtc atcgagcaag gccctgagct cccgctcgcc ggccgccacc ctggcggaca
     4921 ggcgccgctc ctcgctcgcg agggcctgcc gcccccggcg ctcgaggtac aggcggttcg
     4981 cgaagaacag agcggcgacc atggtcagca gtcccatggc gccgaccagg aagcgcggcc
     5041 tatcgatggc ctcgagctcg gacaataggg acgcgcggga ctcgtcggct atgatcgtga
     5101 actccccatc gccgagcgcg aacgagcgcg aggtcatcag gctgccctgg tccagctcga
     5161 agaggctcgg gttacgatcg tcggaggccc agagtacgcg acccgccccg aacatcagga
     5221 acgaacgccg cccctcgcct tcagccagcg gcgacaggta cgtgtcgatg ctgcgtccga
     5281 ggccgatagc ggtcgcgagg atacccacca tgtcgccacc gacgtgcacc gggaacagcg
     5341 ccatgaaata cggctcgggc cgattcgacc ggcttgaaag cacgaccggg tcgctcatgc
     5401 ccaggaagcc atcccattgg gcgacggagg cgaagaccag ggcctccatg gtctcctcgg
     5461 ctcccggccg cgcccaggct agctcaagcc tgcctggccc ggtcaggtag cagtacgata
     5521 gcgactcggg atacgcatcg ccctcggctt cgagcaaggc ctccatggaa gccctgccgc
     5581 gcaggccacg cgcgtactcg gggaacgaat acgcggccag cacgcggttc tcctggagca
     5641 agcgctcgaa gccctcttcc atcgcgttac gcgccagttc gacctgcagg gcgcgattac
     5701 gctcggccat ctcgaggtgc gtgcgcacgg acgccctgtc gatgacaatc caggcgagcg
     5761 ctatcgccgt cgcgatc
//
LOCUS       DAOPHV010000097         5772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_18296_length_5772_cov_4.389890, whole genome shotgun sequence.
ACCESSION   DAOPHV010000097 DAOPHV010000000
VERSION     DAOPHV010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18296_length_5772_cov_4.389890"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="PLQ29_03875"
     CDS             complement(<1..1093)
                     /locus_tag="PLQ29_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG85816.1"
                     /translation="MNMGIRAKLWFGFISIAAIGVIIGAVGIVALTSMNKADTALYEK
                     ATLPVQYSGNFSTLVGRLNGAILASIIDKTPGALERYTTARGNYFKQFYETIRLYNAA
                     VETEADHKRLAELTKLVETLEREDAMVYAFIAAGNLDAAAKLWAGEYLVAYYAVQDVT
                     NSMADENVEKAKATSEGNDRLAIQSTIILLASIAAGLAVALLMSWLLSRSILKIVNVI
                     DSNAQGVAEGIGQVSNASEMLAQGSSEQASSIEEVSASIEELSATIRQNADNAGQTEK
                     IATKSANDARESGEAAAQTVTAMREISERILVIQSIASQTNLLSLNAAIEAARAGEHG
                     RGFAVVASEVQKLAERSSTAAREIEELSKS"
     gene            complement(1090..3243)
                     /locus_tag="PLQ29_03880"
     CDS             complement(1090..3243)
                     /locus_tag="PLQ29_03880"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HPG85817.1"
                     /translation="MNFDDNKELFVEEARDLLAKMESALLSLEKDPGSGELINEVFRA
                     LHTIKGSGAMFGFDGMSAFAHGFENMFDDMRKGLVGVSRDIIGLSLRSLDCLSAWLDS
                     GEAHKDQAAIERELEAIKATLRSGSSPAATKPAAEPAAEPAVAPTVGRGRADVDSDGL
                     TNLYRIRFKPNRDLLTRGVKVENLFGDLAELGQYYAYADVDAVPCLDAIDPTSLYLSW
                     IIDLSTAAPVEDIRSVFIFVEDYAEIGIERLEVVDQTGKPSVPRIGEILANRGALAPD
                     DIDAIHRKQKPFGELAVESGKLSKPALEAALAEQRMAKAAAGERESRKESSNIRVRKE
                     KLDYLIDAVGELVILEARLRQEARVAGATAFESIAENLGRLTSDLRDATMNIRMVPLE
                     ESFSGFHRLVRDLASQLGKEIDLVISGANTEMDKNIIEALKDPLVHIIRNSADHGIEA
                     PELRAKLGKSRAGTIRIEARQQGSKVEIAISDDGAGLDLERIKAKGVERGLIDATEKD
                     PKRIMPLIFEPSFSTAARTTGVSGRGVGMDVVKSNLERLRGEIAISSESGLGTRILLS
                     IPLTLVIVDGLLVRVGDVMYVLALNQVEECVDVACSEVLGDGRRGIINLRGKTIPIID
                     LRDAFGLDAADDAGLQRLVIVDAEGMTVGFRVDGVIGKQQVVIKPFTMGLRQVPTIAG
                     ATILGDGSVAFILNIRELFKSQRQSEPLARERATT"
     gene            complement(3240..3494)
                     /locus_tag="PLQ29_03885"
                     /pseudo
     CDS             complement(3240..3494)
                     /locus_tag="PLQ29_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
     gene            complement(3637..5055)
                     /locus_tag="PLQ29_03890"
     CDS             complement(3637..5055)
                     /locus_tag="PLQ29_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPG85818.1"
                     /translation="MIRAHHSPDDPGGEGSGLSFSADVRKNDGNIEITVSADMMTATA
                     TLYPPIGDGAPLTPDYAAELLARMGIHSGLLWDELSERIFDVNADRRVIRDVVVARGS
                     PPVSEHPEHIVVDDRFLPGFRPVSDEEHTVDWKAISQVLVVKQGEGIGRVIPRRDGAN
                     GCDVTGNPIAFGKDQVTTYALGKNVERDGDDIRAVCDGRVTLDGQRISVEEVLVIKGD
                     VDYRVGHIMFPGDVVIEGGVAAGFKVYSGGSISIKETMDAFDVSAKRDLICAQGIIGK
                     DLGQVRVGGSLKAKFIENARVAVRGDVEVPGSIVGSSIYTLGRLAMGDKGRIVGGEAF
                     ATHGVSCGFLGGGTKPVTIVNVGIDFTIQQKLEQANAALRALTIRLARLQNVRKLRPD
                     DEPLAKAVEEARAKLQSLTDNVADLTRRVDIDEGASVEVKGIVYPGVIVTICHIRVNV
                     DEPLKKARFRLDRAANKIIIER"
     gene            5160..5636
                     /locus_tag="PLQ29_03895"
     CDS             5160..5636
                     /locus_tag="PLQ29_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HPG85819.1"
                     /translation="MAKRRERLKVILKLLRENRVESQEEMLVLLEAHNIFITQATLSR
                     DLKLLKASKVGAGSEGYFYAVPSDDELRRREEIHGQDFIRGYVSIDWNDAFVVIKTFS
                     GHSAPVALALDNLGLEGVLGTIAGQDNVVFAALRNGYSGEDFLRDLRARVPELDED"
BASE COUNT          919 a         1854 c         1877 g         1122 t
ORIGIN      
        1 tggatttgga cagctcctcg atctccctcg cggcggtcga gctacgctcg gccagcttct
       61 gcacctcgct cgccaccacg gcgaaacctc gcccgtgctc tccggccctg gcggcctcta
      121 tggccgcgtt gagcgacagg aggttcgtct ggctggctat cgactggatg accaggatac
      181 gctccgatat ctcccgcatc gccgtcacgg tctgcgccgc cgcctctccc gactcgcggg
      241 cgtcgttggc cgacttggtg gctatcttct cggtctggcc ggcgttgtcg gcgttctgac
      301 gtatcgtcgc cgacagctcc tcgatgctcg ccgatacctc ctcgatgctc gaggcctgct
      361 cgctcgagcc ctgggcgagc atctctgacg cgttcgacac ctggcctatc ccctcggcga
      421 cgccctgggc gttcgagtcg atgacattga cgatcttcag tatggaacgg ctcaggagcc
      481 acgacatcag gagcgcgacg gctaggccgg cggcgatgga cgccaagagt atgatcgtcg
      541 actggatcgc gagcctgtcg ttgccctcgg acgtggcctt ggccttctcg acgttctcgt
      601 cggccatgga gttggtaacg tcctgtaccg cgtaataggc gaccaggtac tcgccggccc
      661 agagcttggc ggccgcgtcc aggttgccgg ccgctatgaa ggcgtagacc atggcgtcct
      721 cgcgctcgag ggtctcgacg agcttggtaa gctcggccag gcgcttatgg tcggcctcgg
      781 tctcgacggc cgcgttgtag agcctgatcg tctcgtagaa ctgcttaaag tagttgccgc
      841 gagccgtcgt atagcgctcg agggccccgg gcgtcttgtc gatgatcgac gcgaggatgg
      901 cgccgttcaa gcgaccgacc agggtggaga agttgcccga gtactgtacg ggcagggtag
      961 ccttctcgta cagcgccgtg tcggccttat tcatgctggt cagcgccacg atgccgaccg
     1021 cgccgatgat gacgccgatg gcggcgatgg agatgaaccc gaaccatagc ttggcgcgta
     1081 tacccatgtt catgtcgttg ctctttcccg ggcaagcggc tcagattgcc gttgggactt
     1141 gaaaagctct ctgatgttca agatgaaggc gaccgagccg tcgccgagta tcgtcgcgcc
     1201 ggctatcgtc ggcacctgcc tgaggcccat cgtgaagggc ttgatgacca cctgttgctt
     1261 accgatgacg ccgtcgacgc ggaagccgac cgtcatgccc tcggcgtcga cgatgaccag
     1321 gcgctggagt cccgcgtcgt cggccgcgtc caggccgaag gcgtcgcgca ggtcgatgat
     1381 ggggatggtc ttgccgcgca ggttgatgat gccgcgccga ccgtcgccga gtacctcgga
     1441 gcatgcgacg tcgacgcatt cctccacctg gttgagcgcc agcacgtaca tcacgtcgcc
     1501 gacccgcacg agtaggccgt cgacgatgac gagggtgagc ggtatggaca ggaggatgcg
     1561 cgtgcccagg cccgactcgg aactgatagc tatctcgccg cgtagccgct cgaggttgct
     1621 cttgaccacg tccatgccga cgccgcggcc cgagacgccg gtcgtcctgg cggcggtcga
     1681 gaacgacggc tcgaagatga gcggcatgat gcgtttgggg tccttctccg tcgcgtcgat
     1741 cagaccgcgc tcgacaccct tggccttgat ccgctcgaga tccagaccgg cgccgtcgtc
     1801 cgagatggct atctcgacct tcgagccctg ctgcctggcc tcgatgcgta tcgtgccggc
     1861 acggctcttc ccgagcttgg ctcgcagctc cggcgcctcg atgccgtggt ccgccgagtt
     1921 tcggatgatg tgcacgagcg ggtctttgag cgcctcgatg atgttcttgt ccatctcggt
     1981 gttggcgccg gagatgacca ggtcgatctc cttgcctagc tgcgaggcta gatcgcgtac
     2041 caggcggtgg aagcccgaga acgactcctc gagcggtacc atgcgtatgt tcatcgtggc
     2101 gtcgcgcagg tcggaggtca gccgcccgag gttctcggcg atgctctcga aggcggtagc
     2161 cccggcgacc ctcgcctcct ggcgtagccg ggcttcgaga atcaccagct ctccgaccgc
     2221 gtcgatcagg tagtcgagct tctccttgcg aacgcggatg ttcgacgact ccttgcgcga
     2281 ctcgcgctcg ccggccgccg ccttggccat gcgttgctcc gccagggcgg cctccagggc
     2341 cggtttcgac agcttgccgc tctccacggc cagttctccg aacggcttct gtttccggtg
     2401 gatggcgtcg atgtcatccg gggccagggc gccgcggttc gccagtatct ctccgatgcg
     2461 ggggacgctc ggcttgccgg tctggtcgac gacctcgagc ctctcgatcc cgatttcggc
     2521 gtagtcctcg acgaagatga acaccgaccg tatatcctcg acgggggcgg ctgtcgacag
     2581 gtctatgatc catgagaggt acaggctggt cggatcgatc gcgtcgaggc acggcaccgc
     2641 gtcgacgtcg gcgtatgcat agtactgacc gagctcggcg aggtcgccga acaggttctc
     2701 gaccttgacg ccgcgcgtca gcaggtctcg gttcggcttg aaccgtatgc ggtacaggtt
     2761 cgtcaagcca tcggagtcga cgtcggcgcg gccccggcct acggtcggcg cgacggccgg
     2821 ttcggcggca ggttcagcgg ccggtttcgt ggccgcgggg gacgacccgg atcgtagcgt
     2881 cgccttgatc gcctcgagct cgcgctcgat cgcggcctgg tccttgtgcg cctcgcccga
     2941 gtcgagccag gcggataggc agtcgagcga acggaggctg agcccgatga tgtcgcggct
     3001 aacgccgacg aggcccttgc gcatgtcatc gaacatgttc tcgaagccat gggcgaaggc
     3061 ggacatgcca tcgaagccga acatggcgcc tgaacccttg atggtgtgca gggcgcggaa
     3121 cacctcgttg atcagctcgc cgctacccgg atccttctcg agcgacagca gggcggactc
     3181 catcttggcc aggagatcgc gggcttcctc gacgaatagc tccttgttgt cgtcgaaatt
     3241 catggcgacg ggctcgtggt ccgctcgata tgatcgaagt ggaagcctat ggtctcgagc
     3301 gacgcgcgta gcggatggcg ctcgtccagc tcgacgatgc ggaattccct gccggcggcc
     3361 tcgagggtct ttgaaagcgc tacgagcaac tgtggtaccg tcacgtcgta gcgcccgaga
     3421 gcgctcaggt ccacggcgac gcgcgccgag gcggacccgg ccgcctcgcg taaggaagag
     3481 gcgagccccg cggcgtcccg gacggtcgac tctcaggtga gctcgatcgt cgtcgtgtcg
     3541 cctttcgcgc ttgttcttgt cggcatgcta ctaggataag ccaggcaacc agtgggtgca
     3601 agcgcggacg gcgaatttta ttgagcggcg agccggctag cgctcgatga tgatcttgtt
     3661 ggccgcccgg tccagcctga acctggcctt cttgaggggt tcgtcgacgt tgaccctgat
     3721 atggcagatg gtgacgatga cgccggggta gacgatgccc ttgacctcga ccgaggcgcc
     3781 ttcgtctatg tcgacgcgcc tggtcaggtc ggccacgttg tcggtcagcg attgaagctt
     3841 ggcccgagcc tcctcgacgg ccttagccag gggctcgtcg tcaggccgta gcttacggac
     3901 gttctgcaac ctcgcgaggc gtatggtcag cgcgcgcagc gcggcgttcg cctgctccag
     3961 cttctgctgg atggtgaagt cgatgccgac gttgactatg gtgactggct tcgtgccgcc
     4021 gcctaggaag ccgcagctga cgccgtgggt ggcgaacgcc tcgccgccta cgatgcggcc
     4081 cttatcgccc atagccaggc gcccgagcgt atagatcgag gagccgacga tggagcccgg
     4141 cacctccacg tcgccgcgta ccgccacgcg tgcgttctcg atgaacttgg ccttgagcga
     4201 gccgccgacc ctgacctggc ccaggtcctt gccgatgatg ccctgggcgc agatcaggtc
     4261 gcgcttggcg ctgacatcga acgcgtccat cgtctccttt atggagatcg agccgcccga
     4321 gtagaccttg aagccggccg cgacgccacc ctcgattaca acgtcgccgg ggaacatgat
     4381 gtggccgacg cgatagtcga cgtcgccctt gatgacgagc acctcctcga cgctgatgcg
     4441 ctggccgtcc agggtcacgc ggccatcaca tacggcgcgg atatcgtcgc catcgcgctc
     4501 gacgttcttg ccgagcgcgt acgtggttac ctggtccttg ccgaaggcta tcggattgcc
     4561 ggtcacgtcg caaccgttag cgccgtcgcg gcggggaatg acgcggccta tgccttctcc
     4621 ttgcttgacg acgaggacct ggctgatagc cttccagtcg acggtatgct cctcgtcgga
     4681 cacgggcctg aagccgggca ggaagcgatc gtcgaccacg atatgctccg ggtgttccga
     4741 caccgggggc gagcccctgg ccacgacgac gtcgcggatg acgcggcgat cggcattgac
     4801 gtcgaatata cgctcggaca gctcgtccca gagcaggccg gaatgtatgc ccatgcgcgc
     4861 cagtagctcg gcggcgtagt ctggagtcag gggcgcccca tcgccgatgg gaggatacag
     4921 ggtggcggtg gcggtcatca tgtcggccga aaccgttatc tcgatgttgc cgtcgttctt
     4981 gcgcacgtcg gccgagaacg agagtccgga accctctccg ccgggatcgt cggggctatg
     5041 atgggcgcga atcacgatgg cgttcctccc tcgttatact atcggtacgg aatcgctccg
     5101 gcttgccaaa aaaccgccgt cgcggcatac tgtcgtatcg tatgcgcgac gcttcatcga
     5161 ttgcgaaaag gcgggaacgc ctcaaggtga tactcaagct cctcagggag aacagggtcg
     5221 agtcccagga ggagatgctc gtcctcctcg aggctcacaa tatatttata actcaggcga
     5281 cgttgtcacg cgacctgaaa ctgctcaagg ccagcaaagt cggggccggc tccgaaggct
     5341 acttctacgc ggtgccctcg gacgacgaac tcaggcgccg cgaggagatc cacggccagg
     5401 acttcatccg cggttacgtc tcgatagact ggaacgacgc cttcgtcgtg atcaagacct
     5461 tctccgggca ttcggcgccc gtggccctgg ccttggacaa cctggggctc gagggcgtcc
     5521 tcggcaccat agcaggccag gacaacgtcg tgttcgccgc cctgcgcaac ggctactcgg
     5581 gcgaggactt cctgcgcgac ctacgggccc gcgtgcccga gctggacgag gactgaccgc
     5641 cccggctacc ctcatggctc ccctttgggc tcccctttgg gccggccgcc gggcgcctta
     5701 tagcctgtca tagcctcgtg ctcctcgagc acgtgctcga gaaggtcgcc tagcttccag
     5761 ccgaggttcg tc
//
LOCUS       DAOPHV010000098         5723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_18525_length_5723_cov_4.380381, whole genome shotgun sequence.
ACCESSION   DAOPHV010000098 DAOPHV010000000
VERSION     DAOPHV010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18525_length_5723_cov_4.380381"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLQ29_03900"
     CDS             complement(<1..325)
                     /locus_tag="PLQ29_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPG85820.1"
                     /translation="MGTSDAARIREEYEKQIFDLKQLLEVSKSLNSTLDYGILIESIL
                     YTIMGQMKVLKAALYAKKGIDSTRFNLHRNYKGFEIEHGRDYSIPEDAPAMRAFARET
                     RCWTMA"
     gene            complement(382..621)
                     /locus_tag="PLQ29_03905"
     CDS             complement(382..621)
                     /locus_tag="PLQ29_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85821.1"
                     /translation="MARAARAWSSSPDAALSAASGCLGVEARTDALELAEAAAPDGAG
                     RSGYIVAIRREDRERLEETMSGLRVRWIGETIRWR"
     gene            complement(735..2519)
                     /locus_tag="PLQ29_03910"
     CDS             complement(735..2519)
                     /locus_tag="PLQ29_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85822.1"
                     /translation="MRISAFGRAAAMAFVYIAVFVVLVMVQFPSGGPITEVSGGVSFK
                     GLPDGDGVRSAELVANGLRLVFSERYPLMLSGGAGPDGALYPVAYEAVDDGFIVRFDD
                     GTRLFVNADDEGRASWRLDAKRSKKASATMRYELAYGAALLAPGDDGSIRLSFGDATY
                     RVSGVTTGSEPRTLSLKATGGAFRAFASIRETKDAAETPAQFLAQAPMDPALWSREIG
                     AWRDKAWSSVSGADFDAASGTWSGAFDEPSFVLYLAEAMRRDLRDAAAALVAVARSSH
                     ADSLSWKSAPFAGKTATSMAAFEAANLAEVKATERLVQARAGTMFYRRGIVPLLFDRA
                     PYSLAQEAMSLARSTDFSKADATQAAALLEAYLDAAGYLDEADNPFSRAVELVDRTIA
                     PAIKKAEGGFFLQTDGDGRCDLLTGLYAGKALIRLSESSGKAIYAGIGQSLVTSAVKL
                     AAADGSIPASVTATGGMLTKSPERLSAAMIYPVVADSPYYPRAVSLYRQLGPGAWAWT
                     CSPSVKAQASPETTVISADYPVGSSHYMALYGVKPYVKIQLYGLDYNMDASFENYNAS
                     GYFYKKAAGAMYLKMRHKVQGEEIRLFY"
     gene            2664..4106
                     /locus_tag="PLQ29_03915"
     CDS             2664..4106
                     /locus_tag="PLQ29_03915"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG85823.1"
                     /translation="MRLRTATRSAAALAVILLASCAGLGSRSGPTEPPSLPGAAGTLV
                     PAELSAEEKERMALDALGRAMDSGDYESALGRLVELVELDPGNRSYPALRSALCLSLG
                     RTDEARALLSAELAAYPDNVEALYALAELERFVGNTGAHRAAIEAIVARHPGDSDALT
                     SLGDIHYDAKNYRKAEESYDAALAAEPGNAEALKGLARVKFRRDDAKGALALLDKAVA
                     AAPDDAIAYLDRSRALYELGRYAECEADMDKAVELAPGSSWNYVERGRLYLDTGRLEL
                     AEADLSESIRLNPDYFLPYVYRASIRENSGDDEAALADYKAAARLYPDYWYSLESMGV
                     LAYRLGDWKTAFASFDGAAGYTKAHPEYYVAAALSLYRGGDARAAKEYAGKKLPLIDR
                     GKHQVHWLALRLVYDQGEMTAEAELAIGAEKSFDIKAGALFYLGSYWIAKGRQELGLK
                     YLRLSYDSERIGTIERRMAEADLARLAAGD"
     gene            4128..5318
                     /locus_tag="PLQ29_03920"
     CDS             4128..5318
                     /locus_tag="PLQ29_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPG85824.1"
                     /translation="MSDTIKALELSGRRIVLVGTAHVSRESVDEVAATIRDEAPDGVC
                     VELDEARWRSMSAPDAWEKTDIVKVIKEGRAFLLLANLALSSFQRRMGMDSGVKPGAE
                     MVAAIRAAEAAGIPFGFCDRDVQTTLRRAWGKSNLWNKSKLLAQLVAAAFSNEKPDAE
                     EIERLKVRSELDEMMGELADYLPSVKEVLIDERDRYIASKIWTMRGERLVAVIGAGHA
                     PGVEAWLARFAEGAPGGDTPGTDVSDLELVPPPGLLARSAGWLVPALIVGFIALGFFT
                     SGAEASLRLLLRWVALNGTLAAIGSVLCLSHPLTVLVSFAAAPIATLNPLVAVGFFSG
                     IVEAWLRKPQVSDFQTLSTDVSSLKGFYRNKVTHILLVFFLSSLGGALGNFIALPFLA
                     GGAF"
     gene            complement(5315..>5723)
                     /locus_tag="PLQ29_03925"
     CDS             complement(5315..>5723)
                     /locus_tag="PLQ29_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPG85825.1"
                     /translation="VPACAPGYPAPDGPARRRRTEAAVRFGDVEIDPERAEVRKAGIP
                     VTLTVQEFRLLMFLVENEGRTLSRACLLEEVWGYDGAIASRTVDVHISSLRQKLGDEP
                     SDSRRIVTIRGFGYRFDSNPQGEPRREPRREPR"
BASE COUNT          917 a         1908 c         1928 g          970 t
ORIGIN      
        1 cggccatggt ccaacagcgg gtctcccgcg cgaaggcgcg catcgccggg gcgtcctccg
       61 ggatgctgta gtcgcgcccg tgctcgatct cgaagccctt atagttacgg tgcaggttga
      121 agcgggtcga gtctatgcct ttcttggcgt acagggcggc cttgagcacc ttcatctggc
      181 ccatgatcgt gtagagaatc gactctatca ggatgccgta gtcgagggtg gaattcaggc
      241 ttttagatac ctcgaggagc tgcttcaggt cgaatatctg cttttcgtac tcttctcgta
      301 tacgcgcggc gtcgctggtt cccatctatc gtctcacatt ctgaatagtg gctttacgca
      361 tagtatacgt cggcatcatg attagcgcca ccggattgtc tcgcctatcc agcgcacccg
      421 caggccgctc atggtctcct cgaggcgctc gcggtcttcc cgcctgatgg cgacgatata
      481 cccgctccgt ccggcgccat ccggcgccgc ggcctcggcc agctcgagcg cgtcggttcg
      541 ggcctctacg ccaaggcagc ccgacgccgc cgacaacgcg gcgtcgggcg agcttgacca
      601 ggccctggcg gcccgggcca cgcggccggc ggccgccctg gctatggccg cttcgacggc
      661 ctcggaccga tctccttcga cgaggtagac gagctcgccg gccgtctgga aggtcgaggg
      721 gaggggacgg ccccctagta gaatagccgt atctcttctc cctggacctt gtgccgcatc
      781 ttgaggtaca tggccccggc cgccttctta tagaaatagc ctgaggcgtt gtagttctcg
      841 aagctggcat ccatgttgta gtcgagcccg tagagctgga tcttcacgta gggcttcacg
      901 ccgtagagcg ccatatagtg cgacgagccg acagggtagt cggcgctgat caccgtggtc
      961 tcggggctgg cctgcgcctt gaccgacggc gaacaggtcc aggcccaggc gccggggccg
     1021 agctgtctgt acaggctgac ggcccgcggg tagtacggcg agtcggcgac gactggatag
     1081 atcatcgcgg cagagagccg ctcgggcgat ttggtaagca tgcctccggt cgccgtgacc
     1141 gaggccggta tggagccgtc ggccgcggcc agcttgacgg cgctcgtcac gaggctctgg
     1201 cctatgccag cgtagatagc cttgcccgac gactcggaca gacggatcag ggccttcccg
     1261 gcgtagaggc cggttaggag gtcgcaacgg ccgtcgccgt cggtctgcag gaagaatccg
     1321 ccctcggcct tcttgatagc cggcgctata gtcctgtcga cgagctcgac agcccggctg
     1381 aaggggttgt cggcttcgtc gaggtagccg gccgcgtcga ggtaggcctc gagcagggcc
     1441 gcggcctggg tggcgtcggc cttggagaag tcggtcgacc gggccaagga catggcttcc
     1501 tgggccagcg agtagggggc gcggtcgaag aggagcggca ctatgccgcg acggtagaac
     1561 atcgtgccgg cgcgggcctg tacgagccgc tcggtcgcct tgacctccgc caggttggcc
     1621 gcctcgaagg cggccatcga ggtagcggtc ttgccggcga acggcgcgct cttccagctc
     1681 aggctgtcgg cgtgcgatga gcgcgcgacg gcgaccaggg ccgcggcggc gtcgcgcagg
     1741 tcgcggcgca tggcctccgc caggtacagg acgaaggacg gctcgtcgaa cgcgccggac
     1801 caggtcccgc tcgcggcgtc gaaatcggcg cccgacacgg aagaccaggc cttgtcgcgc
     1861 caggcgccta tctccctgga ccagagggcc gggtccatcg gggcctgggc aaggaactgg
     1921 gccggggtct ccgccgcgtc cttggtctcg cgtatcgaag cgaaggccct gaacgccccg
     1981 ccggtcgcct tgagcgacag cgtgcgcggc tcggaaccgg tggtgacgcc ggatacccgg
     2041 taggtagcgt cgccgaagct cagccgtatg gaaccgtcgt cgcccggggc gagcagagcc
     2101 gcgccatagg ccagctcgta gcgcatcgtc gcggaggcct tcttggagcg cttggcgtcg
     2161 agccgccagg aggcccgtcc ctcgtcgtcg gcgttgacga acagtctcgt gccgtcgtcg
     2221 aaccggacga tgaaaccgtc atcgactgcc tcgtaggcta ccggatacaa cgcgccgtcc
     2281 ggacctgcgc caccgctcaa catcagcggg tagcgttccg agaagacgag cctgaggccg
     2341 ttcgcgacga gctccgcgga tcgtacgccg tcgccgtccg gcagcccctt gaacgatacg
     2401 cctcccgaca cctcggtgat gggccccccg gacgggaact ggaccatcac gagcaccacg
     2461 aagacggcga tataaacgaa ggccatcgcc gcggcccttc cgaacgcgct tattctcata
     2521 tcgatgggac tatacacgtt gccagttcgc ttgcgcaacc gcggactccc gttccggcgg
     2581 ccgttccgtc ggttccctcg cttgctaaag cgctataaac cggctaccat gacgggaatt
     2641 cgaaccctcg accggagaga cgcatgagac tacgaacggc gacgcgctcg gccgccgccc
     2701 tggccgttat actcctcgct tcctgcgcgg ggctcgggag ccgatccggg ccgacggagc
     2761 ctccgtcgct acccggcgcc gccggcacgc tcgtcccggc cgagctgagc gccgaggaga
     2821 aggagcggat ggccctcgac gcgctcggcc gcgccatgga ttcgggcgac tacgagtccg
     2881 ccctcggacg cctggtcgag ctggtcgagc tggatcccgg caaccgctcg tatccggcgc
     2941 tacgctcggc gctatgcctg tcgctcggtc ggaccgacga ggctcgcgcc ttgctctcgg
     3001 cggagctggc cgcctacccg gataacgtcg aggctctgta cgcgctggcg gagctcgagc
     3061 gcttcgtcgg aaacaccggg gcccaccgcg ccgcgatcga ggccatcgtc gcgaggcatc
     3121 caggagattc cgacgccctt acctccctcg gcgatatcca ttacgacgca aagaattacc
     3181 gaaaggccga ggagtcgtac gacgcggcgc tcgccgccga gccaggcaac gccgaggccc
     3241 tcaagggttt ggctcgggtc aagttccgca gggacgacgc gaaaggcgcc cttgccctgc
     3301 tcgacaaggc cgtcgcggca gcaccggacg acgcgatcgc ctacctggac cgcagccgcg
     3361 ccctctatga gctgggccgc tacgccgagt gcgaggccga catggacaag gccgtcgagc
     3421 tcgcgcccgg ctcgtcctgg aactacgtcg agcgcggccg gctctacctg gataccggcc
     3481 gcctcgaact cgcggaggcg gatctctccg agtccatccg cctgaatccg gattatttcc
     3541 tgccctacgt ctacagggcg tcgatacgcg agaattccgg cgacgatgag gcggcgctcg
     3601 ccgattacaa ggcggcggcc cggctgtacc ccgattactg gtactcgctc gagtcgatgg
     3661 gcgtgctcgc gtaccggctc ggcgactgga aaacggcctt cgcgtcgttc gacggagcgg
     3721 ccggttatac caaggcgcat cccgagtact atgtggccgc ggctctgtcg ctgtaccggg
     3781 gcggcgacgc gagggccgcc aaggagtacg cgggcaagaa gctgccgctc atagaccgcg
     3841 gcaagcatca ggtccactgg ctggcgctgc gcctcgtcta cgaccagggc gagatgacgg
     3901 ccgaggcgga gctggcgatc ggcgccgaga agagcttcga catcaaggcc ggcgcactgt
     3961 tctacctggg ctcctactgg atagccaagg gccggcagga gctcggcctc aagtacctgc
     4021 gtctatcgta cgactccgag cgtatcggca cgatagagcg ccgcatggcc gaggccgacc
     4081 tggcccggct ggcggccggc gattgaatcc gcacgaacgg agtagcgatg tctgatacga
     4141 tcaaggcgct cgagctgtcg gggcgcagga tagtgctggt gggcaccgcc cacgtctccc
     4201 gggagagcgt cgacgaggtg gccgcgacga tacgcgacga ggcgcccgac ggcgtctgcg
     4261 tcgagctcga cgaggcgcgc tggcgctcga tgagcgcgcc ggacgcctgg gaaaagaccg
     4321 acatcgtcaa ggtgatcaag gagggcaggg ctttcctgtt gctcgcgaac ctggcgctgt
     4381 cgtccttcca gcgcaggatg ggcatggact ccggcgtcaa gccaggagcc gagatggtcg
     4441 ccgccatacg cgccgccgag gccgccggca tcccgttcgg tttctgcgac cgcgacgtcc
     4501 agacgacgct gcgcagggcc tggggcaagt cgaatctctg gaacaagtcg aagctcctgg
     4561 cccagctcgt cgcggccgcc ttctcgaacg agaagcccga cgccgaggag atagagcggc
     4621 tcaaggtgcg ctccgagctc gacgagatga tgggcgagct ggccgattac ctgccgagcg
     4681 tcaaggaagt gctcatcgac gagcgggacc gctatatcgc ctccaagatc tggacgatgc
     4741 gcggcgagag gctggtagcc gtgatagggg ccggccatgc gccgggcgtc gaagcctggc
     4801 tcgcccgatt cgccgaaggc gcgccaggcg gcgacactcc gggcaccgac gtatcggacc
     4861 tcgagctggt gcccccgccc ggcctgttgg ccaggagcgc cggctggctc gtaccggccc
     4921 tgatcgtcgg cttcatcgct ctcggcttct ttacctccgg cgccgaggcc agcctgcgcc
     4981 tcctcctgcg ctgggtggcg ctcaacggca cgctggcggc catcggatcc gtcctctgcc
     5041 tgtcccaccc gctgacggtg ctggtatcgt tcgcggcggc gcccatcgcc accctgaacc
     5101 cgctggtcgc ggtcggcttt ttttctggaa tcgtcgaggc ctggctccgc aagccccagg
     5161 tgtccgattt ccagactctg tcgaccgacg tgtcgagcct gaagggcttc tatcggaaca
     5221 aggtcacgca catcctgctg gtcttcttcc tgtcgagcct tggcggcgcg ctcggcaact
     5281 tcatcgcgct acctttcctg gcgggcgggg cgttctagcg gggttccctt cggggttccc
     5341 ttcggggttc cccttggggg ttcgaatcaa aacggtagcc aaagccgcgg atggtgacga
     5401 tccggcgcga gtcggacggt tcgtcgccga gtttttgcct gagcgacgaa atgtgtacgt
     5461 cgaccgtcct cgaggcgatc gcgccgtcgt aaccccagac ctcctcgagc aggcacgcac
     5521 gggacaacgt gcggccctcg ttctcgacca agaacatcag tagccggaat tcctggacgg
     5581 tcagcgtgac cggtatcccg gccttgcgga cctcggcccg ttccggatcg atttcaacgt
     5641 cgccgaagcg caccgccgcc tcggtcctcc ggcggcgggc cgggccatcc ggcgcagggt
     5701 atccgggtgc gcatgctgga acg
//
LOCUS       DAOPHV010000099         5717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_18543_length_5717_cov_3.685447, whole genome shotgun sequence.
ACCESSION   DAOPHV010000099 DAOPHV010000000
VERSION     DAOPHV010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18543_length_5717_cov_3.685447"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="PLQ29_03930"
     CDS             complement(<1..1229)
                     /locus_tag="PLQ29_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPG85826.1"
                     /translation="MVTNDRAGGLRLDFDELPPDPVFEDGIRRAPRREARLTRAQEAL
                     AVKNALRYVPERWHARLAPEFADELRERGRIYGYRFRPEGRLYGKPIDAYAGACVEGR
                     AFQVMIDNNLDFDVALYPYELVTYGETGQVCQNWMQYRLIRQYLERLDQSTTLVIESG
                     HPLGLFKSHELAPRVVITNGLMIGMFDNQKDFNHAAALGVANYGQMTAGGWMYIGPQG
                     IVHGTFNTLLNAGRLKLGVARDGDLRGKLFVSSGLGGMSGAQGKAAVIAGASSIIAEV
                     DESRVVTRHSQGWVDRVEKTVAGAFARAEEARSKGEPIAVAYLGNIVDLLEYADSHGV
                     RIDLLSDQTSCHAAYEGGYCPAGASFEERTRLLAEWFDLLRIPSVGADDAALPHCARA
                     AAWLKRWLKRTGFDAEVL"
     gene            complement(1309..2862)
                     /gene="hutH"
                     /locus_tag="PLQ29_03935"
     CDS             complement(1309..2862)
                     /gene="hutH"
                     /locus_tag="PLQ29_03935"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HPG85827.1"
                     /translation="MKTIVLDGRGLAIGDVALAARFGAKAELAREAIPRMQASRAVVE
                     RCVDESLIRYGITTGFGKFCDVVISRESNAELQRNLIMSHAVGVGDPLPEEVVRAMML
                     LRVNALAVGNSGARLSTAQALLDMLNAGVTPVVPEKGSLGASGDLAPLSHVAIALCGM
                     GECCYKGRRMSSAEALAGAGLAPVELAAKEGLALINGTQCMTALAALAAYDAERLALT
                     ADVVAALTSQALRGIVDAYDPRVHEVRGQPGQIACAARLRALLDGSSYTTRQGQERVQ
                     DAYALRCVPQVHGASRDAIRYVVDAVSRELNAATDNPLIFANPDDPSGGDIISGGNFH
                     GQPVALAMDFLGIALAELANVSERRLERLVNPALSNGLPAFLVRDGGLNSGFMIVQYT
                     AASLVSENKVLAHPASVDSIPSSANQEDHVSMGTIAARKAREILGNARTVLALELLAA
                     CQAIDLLGGPDGLSPATRAAYRALRSVAPMVERDRVMQDDIKAAEALVSDGAALAAAE
                     AAMGFASVG"
     gene            complement(2983..3567)
                     /locus_tag="PLQ29_03940"
     CDS             complement(2983..3567)
                     /locus_tag="PLQ29_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85828.1"
                     /translation="MSRKELEAALDFILNRADPSEFEVLAKACERRRRDMGKYAGLGG
                     LNPGALADKMAASVQEGVGRSLDGLRDTLRDYVARLVRQKEPGATDEQIEALLDECLP
                     DRSDPSSGARDVPAADSLPPEAVAMMVRDFVDYSLGLMPPSRQRELWEGLDAWQDKYW
                     QAFSPELRAFVKARLEGRLAEDEFWSAVFSLLGL"
     gene            3709..>5717
                     /locus_tag="PLQ29_03945"
     CDS             3709..>5717
                     /locus_tag="PLQ29_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85829.1"
                     /translation="MPAKRDFGVTLDRRSPSERGRLIAYINLKLSALGLPVYGKEGTA
                     FLELASDMLLNYREKARLLADYLPPADARIQAFLDSYLADLPDGEAPRLPSNTLTLDR
                     YGMARELSLPPDAHEYRSPTMSSYRIRNGILHNPKNDRRTTKGVFHIAEGGLPVPLDK
                     LAVPKLAFARMLTAALAPPDELLVLPFTAQEAEPARCFLSLLMRPTVRPEVPGLYEER
                     SMETRFFAPASLAANLDFVESIFGNSGDPYVSANDAALDPLHWSGHTGCVILATHLVG
                     MPKKALGLPSWDEATERQRRDGMAWKDPSEPYNGGQPFKLCARDERGVIVTIIADNYF
                     GYSKKEIKTQISYSSNLLGLTEEEHAGGALVLPSFNLGNRFVPDTNLRSKGQRFDDVV
                     RLLGDRIELRPEGYAIDAVFPSVVYLPEDAVLSIDAQKATWRRDGVEQSLRILPGETY
                     VHPTGYRIAMNQHPKTGAWRLVGTQADGLLCHKPCTVSGGGKSEISKQITDAMSDGPI
                     VTGDFAADMAMVREVIEYPYGTRFKDPGENHGSASRSILSDKRSLGSVIKLLSPSELY
                     TEAYNAWLASIPERVKALVFLVKRFYRPEWGDDWAGHFSTDVVNGAAGNVVKYDGRPV
                     QGSYLRVGFTPDGARSIHKLRQDYMPAAKIQWEDDITASVTVPARG"
BASE COUNT          938 a         1996 c         1882 g          901 t
ORIGIN      
        1 aggacttcgg cgtcgaagcc cgtccgcttg agccaccgtt tgagccaagc cgctgcgcgc
       61 gcgcagtgcg gcagcgccgc gtcgtcggcc ccgaccgacg ggatgcggag caggtcgaac
      121 cactccgcca gcaggcgggt gcgctcctcg aacgaggcgc ccgcggggca gtacccgccc
      181 tcgtacgcgg cgtggcagct cgtctggtcg gacaggaggt cgatccgcac gccgtgggag
      241 tcggcgtact cgagcaggtc gacgatattg cctaggtagg cgaccgctat cggctccccc
      301 ttcgaacggg cctcctccgc ccgggcgaag gcgccggcca ccgttttctc gacgcggtcg
      361 acccagccct gggaatgcct cgtgacgacg cggctctcgt cgacctcggc gattatggag
      421 ctggccccgg ctatgacggc ggccttgccc tgggcgccgg acatgccgcc aaggccggac
      481 gagacgaaca gcttgccgcg gaggtcgccg tcacgcgcga cccccagctt gaggcggccg
      541 gcgttgagca gcgtattgaa ggtaccgtgg acgatgccct gcgggcctat gtacatccag
      601 ccgccggcgg tcatctggcc gtagttggcc acgccgaggg ccgccgcgtg gttgaagtcc
      661 ttctgattgt cgaacatgcc tatcatcagg ccgttcgtga ttacgacgcg cggcgccagc
      721 tcgtggctct tgaacaggcc gagcggatgg ccggactcga tgacgagggt cgtcgactgg
      781 tcgagccgct cgaggtactg cctgatgagc cggtactgca tccagttctg gcagacctgg
      841 ccggtctcgc cgtaggtgac gagctcgtag ggatagaggg ccacgtcgaa gtcgaggtta
      901 ttgtcgatca tcacctggaa ggccctaccc tcgacgcagg cgccggcgta ggcgtctatc
      961 ggcttgccgt agagcctgcc ctccggcctg aagcgataac cgtagatgcg gccccgctcc
     1021 cggagctcat cggcgaactc gggggccagg cgcgcgtgcc atcgctcggg cacgtagcgg
     1081 agggcgtttt tgacggcgag cgcctcctgg gctctcgtca ggcgggcctc gcgccgaggc
     1141 gcacggcgga tgccgtcctc gaacacggga tcgggcggca actcgtcgaa atccagcctc
     1201 aggcctccgg ctctatcgtt cgtgaccatg gtatctcctc gcatcgcagt ctatccatca
     1261 tagcaccccc ggcgatggct gaacagaacg cccgccggcg ataacccctc agccgacgga
     1321 cgcgaatccc atcgccgcct cggcggctgc cagcgctgcc ccgtcggaca cgagggcttc
     1381 ggcggccttg atgtcgtcct gcatcacgcg gtcccgctcg accatcggcg ccaccgagcg
     1441 cagggcgcgg tacgcggccc tcgtggcggg cgagaggccg tccgggccgc cgagcaggtc
     1501 gatggcctgg cacgcggcca gcaactcgag ggccagcacc gtcctggcgt tgccgagaat
     1561 ctctcgcgcc ttacgggccg ctatcgtccc catggagaca tggtcttcct ggttggcgct
     1621 cgacggtatc gagtcgacgc tggccggatg ggcaaggacc ttgttctcgg agacgagcga
     1681 ggcggcggta tactgcacga tcatgaaacc ggagttcagg ccgccatccc gtacgaggaa
     1741 ggcgggcagg ccgttcgaca gggccgggtt gacgaggcgc tcgaggcggc gctccgacac
     1801 gttggccagc tcggcgagag cgatgcccag gaagtccatc gcgagcgcta ccggctggcc
     1861 gtggaagttg ccgccggaga tgatgtcgcc gcccgatggg tcgtcggggt tggcgaagat
     1921 aagcgggtta tcggtcgcgg cgttcagctc ccgcgacacc gcgtcgacga cgtagcgtat
     1981 ggcgtcgcgc gaggcgccgt ggacctgcgg cacgcagcgc agggcgtagg cgtcctgaac
     2041 gcgttcctga ccctgcctgg tcgtatacga cgagccgtcg agcagggcgc ggaggcgggc
     2101 cgcgcaggct atctggcccg gctgcccgcg tacctcgtgc acgcgcgggt cgtaggcgtc
     2161 gacgataccg cgtagcgcct gcgaggtcag cgccgccacc acgtcggcgg tcagcgccag
     2221 ccgctcggcg tcgtaggcgg cgagcgcggc gagggcggtc atgcattggg tgccgttgat
     2281 gagcgcgagg ccctccttgg cggcgagctc gaccggtgcc agacctgcgc cggcgagcgc
     2341 ctcggcggac gacatccgcc ttcccttata gcaacactcc cccataccgc agagagctat
     2401 ggcgacatgg gacaaggggg cgaggtcgcc cgatgcgccg agggagcctt tctcgggcac
     2461 caccggcgtc acgccggcgt tcagcatgtc gagcagggcc tgggcggtgg acagcctagc
     2521 cccagagttg cccacggcga gggcgttgac gcgcaggagc atcatcgctc ggaccacctc
     2581 ctcggggagc gggtcgccga cgccgaccgc atggctcatg atgaggttcc gctggagctc
     2641 ggcgttgctc tcccggctga tgacgacgtc gcagaacttt ccgaagccgg tcgtaatacc
     2701 gtagcgtatc agcgactcgt cgacgcagcg ctcgacgacg gccctggagg cctgcatcct
     2761 cgggatagct tcccgcgcca gttcggcctt cgcgccgaaa cgggccgcca gcgcgacgtc
     2821 gcctatggca agccctcgtc cgtcaagcac tatagtcttc atcgggaacc tcttccgggc
     2881 cccgcctcgc gcgggaccgc gtctcaagct ggatcgggcc gcagtatacc acggggcaga
     2941 ggccggggcg aggcgtcgag gcgattctat cgagcgcggc gctcatagcc cgagcagact
     3001 gaacacggcc gaccagaatt cgtcctcggc gagtcgtccc tcgagcctgg ccttgacgaa
     3061 ggcccgtagc tccggcgaga aggcctgcca gtacttgtcc tgccaggcgt cgaggccctc
     3121 ccatagctcc cgctgcctcg agggcggcat cagcccgagc gagtagtcga cgaagtccct
     3181 gaccatcatc gcgacggcct cgggcggcag gctgtcggcc gctggaacgt cccgggcgcc
     3241 cgacgaaggg tcggacctgt cgggcaggca ttcgtcgagc agcgcctcga tctgctcgtc
     3301 ggtcgcgccg ggctccttct ggcgaacgag gcgggccacg tagtcgcgca gggtgtcgcg
     3361 cagcccgtcc aggctacggc cgacgccctc ctggaccgag gcggccatct tatcagccag
     3421 ggcgccggga ttgagaccgc caagcccggc gtacttgccc atatcccgcc gcctgcgctc
     3481 gcaggccttg gcgaggacct cgaactcgga cgggtccgcc cggttcagga tgaagtcgag
     3541 cgccgcttcc aattctttcc tggacataac gccactatac catcgttcga tcgccgagtc
     3601 tgtcgtcgac cgggcgacta ggcgccctcc tggcaaaaag tcgtcgttca tactacgatg
     3661 gaccggaggc cctgacggcc cgtcccatac gcatgaacgg aggctcatat gccggcaaaa
     3721 cgagacttcg gagtcaccct ggatcgacgc tcgccgtcgg aacggggaag gctcatcgca
     3781 tacatcaacc tgaagctatc ggcactgggc ctcccggtct acggcaagga aggtaccgcg
     3841 ttccttgagc tggccagcga catgttgctc aactaccggg agaaggcgcg gctgctcgcc
     3901 gactatctcc ctccggcaga cgcgcgcatc caggcgttcc tcgactcgta cctggccgac
     3961 ctgcccgacg gcgaggcgcc tcgcctgccc tcgaacacgc tgacgctcga ccggtacggc
     4021 atggccaggg agctgtcgct gccgccggac gcccacgagt accgctctcc cacgatgtcg
     4081 tcgtatagga tacgtaacgg tatcctccat aatcccaaga acgaccggcg cacgaccaag
     4141 ggcgtcttcc atatagccga aggcggcctg ccggtcccgc tcgacaagct ggccgtgccc
     4201 aagctagcct tcgccagaat gcttaccgcc gcgctggccc ccccggatga actcctcgtc
     4261 ctaccgttca cggcgcagga ggcggaacca gcccgctgct tcctgtccct cctgatgcgg
     4321 cctacggtac ggcccgaggt gcccggcctc tacgaggaac ggtcgatgga gacgcgcttc
     4381 ttcgccccgg cctcgctcgc ggccaacctc gacttcgtcg agagcatttt cggcaactcg
     4441 ggcgacccct acgtctcggc caacgacgcc gcgctcgacc cgctccattg gtcgggccac
     4501 accggctgcg tcatcctggc cacgcacctg gtcggcatgc ccaagaaggc gctcggcctg
     4561 ccatcctggg acgaagccac cgagcgccag aggcgcgacg gcatggcctg gaaggatccg
     4621 tccgaaccgt acaacggcgg ccagccgttc aagctctgcg cccgcgacga gcgcggtgtc
     4681 atcgtcacca tcatcgcgga taactacttc gggtactcca agaaggagat caagacgcag
     4741 atctcctact cgtcgaacct gctcggcctg accgaggagg agcacgccgg tggcgccctc
     4801 gtgctgccca gcttcaatct cggcaaccgc ttcgtgcccg acaccaacct gcgctccaag
     4861 ggccaacgct tcgacgacgt ggtgcgcctg ctcggcgacc gcatcgagct acggccggaa
     4921 ggctacgcga tcgacgcggt cttcccgagc gtggtctacc tgcccgagga cgccgtactg
     4981 tccatcgacg cccagaaggc gacctggcgg cgcgacggcg tcgagcagag cctgcgtatc
     5041 ctgcccggcg agacctacgt gcacccgacc ggctaccgca tcgccatgaa ccagcacccg
     5101 aagaccggcg cctggcggct cgtgggcacc caggccgacg gcctcctgtg ccacaagccg
     5161 tgcacggtct cgggcggcgg caagtccgag atctccaagc agatcaccga cgccatgagc
     5221 gacggcccca tcgtcaccgg cgatttcgcc gccgacatgg ccatggtcag ggaggtcatc
     5281 gagtatccct acggcacccg cttcaaggat cccggggaga accacggctc cgccagccgg
     5341 tcgatcctat cggacaagcg ttcgctcggt agcgtcatca agctgctgtc gccttccgag
     5401 ctgtacaccg aagcgtacaa cgcgtggctg gcctcgatcc ccgagcgcgt caaggcgctc
     5461 gtcttcctgg tcaagcgctt ctaccgcccg gagtggggcg acgactgggc cggccatttc
     5521 tccaccgacg tcgtcaacgg agcggccggc aacgtggtca agtacgacgg acggccggtc
     5581 cagggttcgt atctgcgcgt cggcttcacg cccgacggcg ccaggtccat ccacaagctc
     5641 aggcaggact acatgccggc cgcgaagatc cagtgggagg acgacatcac cgccagcgtc
     5701 accgtgcccg cgcgcgg
//
LOCUS       DAOPHV010000100         5650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_18828_length_5650_cov_4.943164, whole genome shotgun sequence.
ACCESSION   DAOPHV010000100 DAOPHV010000000
VERSION     DAOPHV010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18828_length_5650_cov_4.943164"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLQ29_03950"
     CDS             <1..335
                     /locus_tag="PLQ29_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85830.1"
                     /translation="DVYVIPSAGVRLGWFELTGGAAFKVYDAPEPPNYIEASTEGASP
                     FLRAGLDVPIGPVSVGLGARLMVADTYTESTAETVGDAIGEGIAAAIVAVFSIFQLDL
                     GLSYRLTF"
     gene            complement(349..2256)
                     /locus_tag="PLQ29_03955"
     CDS             complement(349..2256)
                     /locus_tag="PLQ29_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPG85831.1"
                     /translation="MNILSLDGVSKTLIGTPLFKDVSLGIEEGERLGLVGRNGAGKST
                     FLRVLSGALEPDTGSIARRRGLRVSVLPQQPAAKPGTKLSEFLYEGDSELVRLVRDYE
                     AAVSGKTGATDRALEALYARMETEGGFLLERRYASLCSEFGLPDIGAPMDGMSGGMVK
                     KAALARTLADSSDLVLLDEPTNHLDLDAIELLERKLGSAAFAFVVVTHDRAFLDAVAG
                     RILEIDRSSVFSYPGDYSAFVAMKAERWNALEKADSRRETILKIEMKWLMRGARARAT
                     KSERRKELIRGMQDSALERPTPMGAFSSKSRRLGKKVLELRGAAKRYGGRLVFEPFSY
                     EFVKGDRIGVVGPNGSGKTTLLGLIAGRLAPDEGSVERGVNTVCSYYGQTAEGLPMDA
                     RMLDFIRSRAELTAMGDGSLLDPERLLERFLFDRTMHDQRLSTLSGGELRRLQLVSVL
                     AEHPNLLILDEPTNDLDIDTIELLEDYVESFDGCVIVVSHDRAFLDGVTSTTIAIDGS
                     GGASLYPYAYGAYREYLEAEAEARGEAERVAAERRQPGVGQQRGRDRSDRSDRAGEPR
                     KPTWAERKEYEGILDEIQALEDEKSSLEALFSSGASGDDLERAGRRYAELCPLIDSRT
                     ARWEELAALIG"
     gene            2353..3378
                     /locus_tag="PLQ29_03960"
     CDS             2353..3378
                     /locus_tag="PLQ29_03960"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HPG85832.1"
                     /translation="MSHRTDPAAPRADYSDLTPELALSAVGEAFGIEPDGSFFAYPSY
                     ANRVYGLRADDGTEYVAKFYRPGRWTPEAILEEHAFVAELEAAEIPAVAPLATPDGDT
                     LPVLAIEGMAGETEYPFALFPKRGGRLFDAERDEDWLRLGALAGRIHAVGQARPFSLR
                     VGIGPGLAGRYADEIVDGGALDPDAVDRVAPLLRRSAALVDEALARAPAPIRLHGDFH
                     RGNVLDRADGGLLAIDFDDASYGPAVQDLWLLLPGPASECRRELGLIVEGYEGFMPFD
                     SRSLALVEPLRLLRMVHFIAWQARQRSDRGFAERWGDWGSKAYWMERALDLADQIERV
                     ESAGPGF"
     gene            complement(3358..4971)
                     /locus_tag="PLQ29_03965"
     CDS             complement(3358..4971)
                     /locus_tag="PLQ29_03965"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPG85833.1"
                     /translation="MAMPVNRSIRSSVPFQFAAVTLFFTALVALLESLLVVRAFSSIE
                     LNGMRDRVAQFDAFLGRERESLANLAKSYAEWGDSYSFMSTRDPAYLENNYNADWIGS
                     QGLDYVLIVDDDGRVVWDSGSSAPAFFPGSAGAWLYRSPLLFSPEYGSRPSASVRSLV
                     ELDGEPVLYASWPITDDLVTVAPRGVLVFARRLDPDALAEYVAGGEFSMGWRSARGSG
                     TLPEPGPVKVSDGGRAYLRGAEPVRDAAGAIIGYWEFSQERNWIRTIRQMVAWFALIG
                     AAGGLGIYAVLHRRVRRLIVVPLMAIKDRLGGFSRTLEALEPLPPFGYEELDELANHV
                     YALTERVCSQTMELDRLASTDGLTGLPNRRSLEKTMDEFRRRRATGKLGVDCRSTSKR
                     GTIAFGMIDVDFFKNYNDLYGHAGGDATLRAIGLVIRECVKRPDDLPSRYGGEEFAVI
                     LPDTDEEGAVAVLERVRQAIEALELPHAGSSAANVVTVSCGAAALRPDGPDGDLDQIM
                     EMADKALYAAKAFGRNRVIAYSSLKPGAS"
     gene            5083..>5650
                     /locus_tag="PLQ29_03970"
     CDS             5083..>5650
                     /locus_tag="PLQ29_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPG85834.1"
                     /translation="MDYSELSMHPDLAKGVQDAGYVTCMPVQAAVFPHAFAGEDIYAQ
                     SQTGTGKTAAFLVSIFQRIMSDSASRHRKVLILAPTRELAVQIEEEAKTLGKHLPISI
                     GSFYGGVAYGPQLDMLRDNVQMLIGTPGRIIDLVQQGKMLLRDVGFLVVDEADRMFDM
                     GFIDDLRKLLRYLPQPKERQTFLFSATLN"
BASE COUNT          911 a         1954 c         1861 g          924 t
ORIGIN      
        1 cggacgttta cgtgattccg agcgccggcg tacggctcgg ctggttcgag ctcacgggcg
       61 gcgccgcctt caaggtctac gacgccccgg agccgccaaa ctacatcgag gcgagcaccg
      121 agggggcctc gccgttcctt cgggccgggc tggacgtgcc tatcggcccc gtctccgtag
      181 gcctgggcgc gagactcatg gtggccgaca cgtatacgga gagcacggcc gagacggtcg
      241 gcgatgcgat cggcgagggc atcgcagccg cgatcgtcgc cgtcttcagt atcttccaac
      301 tggatctcgg cctgagctac aggctgacgt tctagcggcg acgacccgct agcctatcag
      361 ggccgccagc tcctcccagc gggcggtccg cgagtcgatg agcgggcata gctcggcgta
      421 gcgccggccc gcccgctcca agtcgtcgcc ggaggcgcct gacgagaata gcgcctcgag
      481 cgatgacttc tcatcctcca gcgcctggat ctcgtccaag ataccctcgt attccttgcg
      541 ctcggcccag gtgggcttcc tcggttcgcc agcgcgatcg gatcgatcgg atcgatcgcg
      601 gccgcgctgt tggccgacgc cgggctgccg cctctcggcg gcgacgcgct cggcctcgcc
      661 tctcgcctcg gcttcggctt ccaggtattc gcgataggcc ccgtaggcgt acgggtagag
      721 cgacgcgccg ccggagccgt cgatcgctat ggtcgtcgac gtgaccccgt ccaggaaggc
      781 ccggtcgtgg gacacgacga tcacgcagcc gtcgaacgac tcgacatagt cctcgagcag
      841 ctcgatcgtg tcgatatcca ggtcgttggt cggctcgtcc aggatcagca ggttcgggtg
      901 ctcggccagg accgacacca gctggagtcg cctgagctcg ccgcctgata gcgtggacaa
      961 gcgctggtcg tgcatcgtgc ggtcgaacag gaagcgctcg agcaggcgct cggggtcgag
     1021 gaggctgccg tcgcccatgg ccgtcagctc ggcgcgagag cggatgaagt ccagcatcct
     1081 ggcgtccatg ggcaggccct cggcggtctg gccgtagtac gagcagaccg tgttgacacc
     1141 gcgctccacc gagccctcgt cgggcgccag ccggccggct atcaggccga gcagcgtcgt
     1201 cttgccggag ccgttcgggc cgaccacgcc gatgcggtcg cccttgacga attcgtacga
     1261 gaacggctcg aagacgagcc ggccgccata gcgcttggcc gctcccctga gctcgagcac
     1321 cttcttgccg agccgtctgg acttcgacga gaaggcgccc atcggcgtcg ggcgctcgag
     1381 agcggagtcc tgcatgcctc ggatcagctc cttgcgccgc tcgctcttgg tagcgcgggc
     1441 cctggcgccg cgcatcagcc atttcatctc gatcttcagg atggtctcgc gcctggagtc
     1501 ggccttctcg agcgcgttcc agcgctcggc cttcatcgcg acgaaggcgg aatagtcgcc
     1561 cgggtaggag aagaccgacg accggtctat ctcgaggatg cgaccggcga cggcgtccag
     1621 gaaggcgcgg tcatgggtga cgacgacgaa ggcgaacgcc gccgagccga gtttacgctc
     1681 gaggagctcg atcgcgtcca ggtccaggtg gttcgtcggc tcgtcgagca ggaccaggtc
     1741 ggacgagtcg gccagcgtcc gggccagggc ggccttcttg accataccgc cggacatgcc
     1801 gtccatcggg gcgcctatgt cgggcaggcc gaattcggag cacagcgacg cgtagcgccg
     1861 ctcgaggagg aagccgccct cggtctccat cctggcgtac agcgcctcga gggcgcgatc
     1921 cgtggcgccc gtttttccgg ataccgctgc ctcgtagtcg cgtaccaggc ggaccagctc
     1981 cgagtcgccc tcgtacagga actccgacag cttggtcccg ggcttggccg ccggctgttg
     2041 cggcaggacc gataccctga ggccgcgcct gcgggctatg gagccggtgt cgggctcgag
     2101 agccccggac agcacgcgta ggaaggtgga cttgccggcg ccgttgcgcc cgacgaggcc
     2161 gaggcgctcg ccttcctcga taccgagcga gacgtccttg aacagcgggg tgccgatgag
     2221 cgtcttggag acgccgtcga gggatagaat attcatgcgt agccggacta tagcccgttt
     2281 cggcccgcag tgtcacctcg ccgcgcggcc cggggcgata ctcccctccc ccggccgtgc
     2341 tatactcggc cgatgagcca ccgcaccgac ccggccgcgc cgcgcgcgga ctacagcgac
     2401 ctgaccccgg agctggccct atccgccgtc ggcgaggcct tcgggataga gccggacggc
     2461 tcattcttcg cgtatccgag ctacgccaac cgagtctacg ggctccgcgc cgatgacggg
     2521 accgagtacg tggccaaatt ctaccgcccc ggccgctgga cgccggaggc catcctcgag
     2581 gagcacgcct tcgtcgcgga gctggaggct gccgagatac cggccgtggc gcccctagcg
     2641 acgccggacg gcgacacgct accggtgctg gctatcgagg gcatggccgg agagaccgaa
     2701 tacccgttcg cgctgtttcc gaaacgcggc ggccgcctct tcgacgctga gcgcgacgag
     2761 gactggctca ggctcggcgc cctcgcgggc cgcatccacg cggtcggcca ggcgcgcccg
     2821 ttctcgctgc gcgtcggcat cggtccaggc ctggccggcc gttacgccga cgagatagtc
     2881 gacggcggcg ccctcgatcc cgacgccgtc gaccgcgtcg cgccgctcct caggcgctcg
     2941 gccgccctcg tcgacgaggc gctcgcccgg gctcccgccc cgatacggct ccacggcgac
     3001 ttccaccggg gcaacgtgct cgatcgcgcc gacggcgggc tcctggcgat cgacttcgac
     3061 gacgcctcct acgggcctgc ggtccaagac ctgtggctct tgctaccggg cccggccagc
     3121 gagtgccgcc gggagctcgg cctgatcgtc gagggctacg aaggcttcat gcccttcgac
     3181 agccgatcgc tggccctcgt cgagccgctc aggctactca ggatggtcca cttcatcgcg
     3241 tggcaggcca ggcaacgttc cgaccgcggc ttcgccgaac gctggggcga ctggggctcc
     3301 aaggcctact ggatggagcg agcgctcgac ctggccgacc agatcgagcg ggtcgagtca
     3361 gctggccccg ggttttaggg acgaataggc gataacgcgg ttacgcccga acgccttggc
     3421 cgcgtacagc gccttgtcgg ccatctccat gatctggtca aggtcgccgt cggggccgtc
     3481 tggccgtagg gccgccgcgc cgcagctgac ggtcacgacg ttcgccgcgc tcgacccagc
     3541 gtgcggcagc tcgagcgcct ctatcgcctg tctgacgcgc tcgagcaccg ccacggcgcc
     3601 ttcctcgtcg gtatcgggca ggatcacggc gaactcctcg ccgccgtagc ggctcggcag
     3661 gtcgtccggc cgcttgacgc attccctgat cacgagcccg atcgccctga gcgtcgcgtc
     3721 gccgccggcg tggccgtaca gatcgttgta gttcttgaag aagtcgacgt ctatcatgcc
     3781 gaaggctatc gtgccgcgct tgctcgtcga gcggcagtcg acgccgagct tacccgtggc
     3841 gcgcctacgc ctgaattcgt ccatggtctt ctcgaggctg cgccggttcg gcaggcccgt
     3901 caggccgtcg gtgctcgcca gcctatcgag ctccatggtc tgcgagcata cccgctccgt
     3961 gagcgcgtaa acgtggttcg ccagctcgtc cagctcctcg tacccgaacg gcggtaaggg
     4021 ttcgagcgcc tccagagtcc gcgagaagcc tccgaggcga tccttgatgg ccatcagagg
     4081 gacgacgatc aggcgcctga cgcgtctatg cagcacggcg tagatgccga gtccgccggc
     4141 cgcgccgatc agcgcgaacc acgcgaccat ctggcgtatc gtccgtatcc agttccgctc
     4201 ctggctgaat tcccagtagc cgattatggc gccggctgcg tcgcgcaccg gctcggcgcc
     4261 tcggagatag gcccgccccc cgtcgctgac tttgacgggg ccaggctccg gtagcgttcc
     4321 agagccgcgg gccgagcgcc atcccatgct gaactcgccg ccggcgacgt actcggcgag
     4381 cgcgtccgga tcgagccgcc gagcgaagac gaggaccccg cgcggcgcga ccgtgaccag
     4441 gtcgtccgtg atgggccagg aggcgtacag gacgggctcg ccatcgagct cgacgaggct
     4501 ccggacggac gccgacggtc tggagccgta ttccggggag aatagcaggg gcgagcgata
     4561 gagccacgcg cccgccgagc cagggaaaaa cgcgggcgcc gaggagcccg agtcccagac
     4621 gacgcgcccg tcgtcatcga cgatgaggac atagtccagg ccctgcgaac cgatccagtc
     4681 ggcgttgtag ttattctcca ggtacgccgg gtcgcgcgtc gacatgaacg agtagctgtc
     4741 gccccactcg gcgtatgact tggcgaggtt cgccagggac tcgcgctccc ggccgaggaa
     4801 ggcgtcgaac tgggccacgc gatcccgcat gccgttcagc tcgatcgacg agaaggcccg
     4861 tacgacgagt agcgactcca atagcgccac caaggcggtg aagaacagcg ttaccgccgc
     4921 gaactggaat ggcaccgacg acctgatcga tctgtttacc ggcatggcca tggttccagt
     4981 ataggcgacg cccgaaccga aataaagctt cggcgaggac ttaacagaga cggccttaaa
     5041 gtgtatccta atagtacttt attgaaggat cgagtgtaac gaatggatta ttcagaactc
     5101 agcatgcacc ccgacctggc caagggcgtt caggacgccg gctacgtgac ctgcatgccg
     5161 gtacaggcgg cggtattccc gcacgccttc gcgggcgagg acatctacgc ccagtctcag
     5221 acgggcaccg gcaagacggc cgcgttcctc gtctccatct tccagcgcat catgagcgac
     5281 tccgccagcc gccatcgcaa ggttctcata ctcgcgccga cgagggagct ggccgtccag
     5341 atagaggaag aggctaagac cctcggcaag cacctcccta tatcgatcgg cagcttctac
     5401 ggcggtgtcg cctacggccc ccagctcgac atgctccgcg ataacgtgca gatgctcatc
     5461 ggcacgcccg gtcgcatcat cgacctcgtc cagcagggca agatgctgct ccgcgacgtc
     5521 ggcttcctcg tcgtcgacga ggccgaccgc atgttcgaca tgggcttcat cgacgacctg
     5581 cgcaagctgc tccgttacct accccagccc aaggaacggc agacgttcct attctcggcc
     5641 accctgaact
//
LOCUS       DAOPHV010000101         5628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_18942_length_5628_cov_5.486991, whole genome shotgun sequence.
ACCESSION   DAOPHV010000101 DAOPHV010000000
VERSION     DAOPHV010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18942_length_5628_cov_5.486991"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(430..1914)
                     /gene="araA"
                     /locus_tag="PLQ29_03975"
     CDS             complement(430..1914)
                     /gene="araA"
                     /locus_tag="PLQ29_03975"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HPG85835.1"
                     /translation="MIDLKEYEFWFVVGSQDLYGDQVLKTVDEHSRKMVQSLDQRLPC
                     RLVWKPVVLGSDEILRLVRAANADPLCAGIVTWMHTFSPSKMWIAGLSELKRPLCHLH
                     TQFNRDIPLDSIDMDFMNLNQSAHGDREHGFIGARMRIPRKVVVGHWEDPAVVGRLSG
                     WMRAAAAFADGKALKVARFGDNMREVAVTEGDKVEAQIKFGWSVNGYGVGDLVESVAS
                     VREPDVDALVQAYGREYELAPEARSGKGAERVREQARLELGMRAFLEAGGFGAYTDTF
                     QDLHGLPQLPGLASQRLMADGYGFGAEGDWKTAGVVRAMKVMAAGLGGVSFLEDYTYN
                     LDPAGPSALSAHMLEVCPSIAAGKPRLEVHPLGIGGKADPARLVFCGEPGPAINATLV
                     DMGGRMRMIVNEVDAIEPKPMPKLPVARVLLRPRPDLARAAECWIIAGGAHHTGYSRV
                     VKTEWMRDWAEMAGVEFLLIDAKTEPEELRKEIRWNEVAYRLGI"
     gene            complement(1911..3581)
                     /locus_tag="PLQ29_03980"
     CDS             complement(1911..3581)
                     /locus_tag="PLQ29_03980"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HPG85836.1"
                     /translation="MTNKKGRDYVIGIDFGSDSVRALVVDAADGEPLGTAVSSYRRWA
                     KGMYCDPKANRFRQHPLDYVESLEECVVAALTNAGAGAASRVRGLTVDTTGSTPCLAD
                     ESGSPLALWPEFSDDPDAMFMLWKDHTSVRQAERINAVARTWGGEDYTKYIGGIYSSE
                     WFWAKALHLVEDGAKAARVAATVIEHCDWMPSLLTGVTRASDIRRGRCAMGHKAMWHP
                     SWGGYPSAAFFDRLDPRLSELAASLGTETYTAEEAAGRLCPEWAGRLGLSTDVVVGVG
                     AFDAHIGSVGGDIAPGTLVKIMGTSTCDVMIGPKPVAGAPERLVAGICGQVDGSVVRG
                     MIGYEAGQSAFGDCFAWLRHLLAWPARAGLLGSGVDPEAFEDRILPALESEAAAIDPA
                     RSGVVALDWLNGRRTPDADQGLTGAIAGLSLGTDAPSLYRALIEAAAFGSRAIVERMR
                     SEGLPVERVVGIGGVARKSPLVMQISADVLGMDIAVSSSDQSCALGGAMFAACVAGLY
                     PSALDAQASMRAPIERTYSPEPGCRAVYDALYAKYKALGAFAEKEMRK"
     gene            complement(3638..4726)
                     /locus_tag="PLQ29_03985"
     CDS             complement(3638..4726)
                     /locus_tag="PLQ29_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPG85837.1"
                     /translation="MEEKITVKEIARLAGVSIGTVDRVLHDRGGVSSKTQARIREIIS
                     SLGYEPDVLARQLSLKKNFTFRVILPRSDQDSGYWALCLQGIRRAELDLARYRCDVRV
                     DEFDRYDEAAYRVLLESVLEDPCDGLLLAPAMPEILCGALARLGSRAGGDGTRDEPGS
                     TPYVFFDGILECASPLAVVAQDPFRGGYLAGRVMSLLSPGAGRLVALSAHAEDRHIAR
                     RIDGFRSFFSDHHSERRIDAVDCPGLDGSSAKEGALERIFGNAADIAGILVANSSGHL
                     VGDWLAARGLKTKLSLVSWDAVPDNVRALADGSIDCLVSQRPAEQAREALELLFHAVV
                     LGEGALASRRPAEIPLELFFRENIPLGE"
     gene            complement(4735..>5628)
                     /locus_tag="PLQ29_03990"
     CDS             complement(4735..>5628)
                     /locus_tag="PLQ29_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG85838.1"
                     /translation="TLGLSIVMIGGGIDISVGAVTGLVTMCCAVFLESGTGSVGGSML
                     LALGIGLAFGLLQGFLVAYLEIQPFIITLSGMFLANGLLTTIHKDPLNVTLPEFVLLR
                     DFELVVPWLGTTNRLGNFIPLAIKPGTVVAVALIALLAVLMRWSRFGRNLYAVGGNSQ
                     SALMLGINVKRAKFGSYVLSGLLSGVAGFVFIMTTGAGNIGNAAGFEMKAIAGSIIGG
                     TMLSGGVGNLIGSPIGALTLLTINELIRAAGVNSNFQSIVSGLLLCIFIVLQSVVLWL
                     RGARGLRRALPAWLTPSGKRG"
BASE COUNT          914 a         1854 c         1901 g          959 t
ORIGIN      
        1 tgtctgggtt gagttctccg tagccaaatc ccgacggctt gatggcgaag actcccgagg
       61 ctggatcgaa ggcggaggcg tttccccagg tgaggatggc cagcctgagc ctgtctatct
      121 cgaggttggc cgcgcaggcc tcctccctga tcgcgtcaaa tttacccaaa gtacacctcc
      181 gcgtaccata atccgattca aaccacggag ccagcgtgag cagtgcggaa cgctgggaga
      241 cacggaggca ataaggagag agaagagaat gagggatacc caagtggtgc atggagtctt
      301 tccatgctct ggcttcgatc atggtttcct atcacgcaag gttttatagt tttcctcttc
      361 acttgggttc ctccctccat ttttttctcc gtgcctctgt gcctccgtgg tatgtatctc
      421 ttcgtatgtc tagatcccca gcctgtaggc cacttcgttc cagcgtattt ccttgcggag
      481 ctcctcgggc tcggtcttcg cgtcgatgag gaggaactcg acgccggcca tctcggccca
      541 gtcgcgcatc cattcggtct tgacgacgcg ggagtagccc gtgtgatggg cgccgccggc
      601 gatgatccag cactcggccg cgcgggccag gtccggcctc ggcctgagga ggacgcgcgc
      661 taccggaagc ttgggcatcg gcttcggctc gatcgcgtct acctcgttga cgatcatgcg
      721 catgcggccg cccatgtcga cgagggtggc gttgatcgcc gggcccggct cgccgcagaa
      781 caccaggcgc gcgggatcgg ccttgccgcc gatgccgagc gggtggacct cgaggcgggg
      841 cttgcccgcc gctatcgacg ggcagacctc gagcatgtgg gcgctcaggg ccgacggccc
      901 cgccgggtcg aggttgtacg tgtagtcctc gaggaaggat acaccgccca gtcctgcggc
      961 catgaccttc atcgcgcgta cgacgccggc ggtcttccag tccccctcgg cgccgaagcc
     1021 gtagccgtcg gccatcaagc gctggctcgc taggcccggg agctgcggca ggccgtggag
     1081 atcctggaag gtgtcggtgt aggccccgaa gccgccagcc tcgaggaagg cccgcatgcc
     1141 gagctcgagc ctggcctgct cgcgcacgcg ctcagcgccc ttgccgctcc gggcctcggg
     1201 ggccagctcg tactcgcgcc cgtaggcctg tacgagggcg tcgacgtcgg gctcgcgaac
     1261 cgaggctacc gactcgacga ggtcgccgac gccgtagccg ttgaccgacc agccgaactt
     1321 gatctgggcc tcgaccttgt cgccctcggt gaccgccacc tcgcgcatgt tgtcgccgaa
     1381 acgggctacc ttgagcgcct tgccgtcggc gaaggccgcg gcggcgcgca tccatcccga
     1441 tagcctgccg acgacggccg ggtcctccca gtggccgacc acgaccttgc gcggtatcct
     1501 catgcgcgcc ccgatgaagc catgctcgcg gtcgccgtgg gccgactggt tcaagttcat
     1561 gaagtccatg tctatcgagt cgagcgggat gtcgcggttg aactgggtgt ggagatggca
     1621 gagcgggcgc ttgagctcgg agaggccggc tatccacatc ttggacgggc tgaaggtgtg
     1681 catccaggtg acgatgccgg cgcagagcgg gtcggcgttg gccgcgcgca cgagcctgag
     1741 tatctcgtcg gagccgagca cgacgggctt ccagacgagg cggcagggca gcctctggtc
     1801 gagtgactgg accatcttcc tcgaatgctc gtcgacggtc ttgaggacct ggtcgccgta
     1861 gaggtcctgg gagccgacga cgaaccagaa ctcgtattcc ttcaggtcta tcatttcctc
     1921 atctccttct cggcgaaggc gccgagggcc ttgtatttgg cgtagagcgc gtcgtagacg
     1981 gcgcggcatc ccggttcggg cgagtaggtc cgctctatcg gggcccgcat cgacgcctga
     2041 gcgtcgaggg cgctcggata gaggccggcg acgcacgcgg cgaacatcgc gccgccgagc
     2101 gcgcatgact ggtcgctcga cgatacggct atgtccatcc cgaggacgtc ggcgctgatc
     2161 tgcatgacga gcgggctctt gcgcgccacg ccgcctatcc cgacgacgcg ctcgaccggc
     2221 aggccctccg agcgcatcct ctccacgatg gccctgctcc cgaaggcggc ggcttcgatc
     2281 agcgcgcggt agagcgacgg cgcgtccgtg ccgaggctga gcccggctat cgctccggtc
     2341 aggccctggt cggcgtccgg cgtgcggcgc ccgttgagcc agtcgagagc cacgactccc
     2401 gagcgcgccg ggtctatcgc ggcagcctcg gactcgaggg ccgggagtat cctgtcctcg
     2461 aaggcctcgg ggtcgacgcc cgatccgagg aggccggccc gggccggcca cgcgagcagg
     2521 tgcctgagcc aggcgaagca gtcgccgaag gctgactgcc cggcctcgta gccaatcata
     2581 ccgcgtacga ccgaaccgtc gacctgcccg cagataccag ccacgagcct ctcgggcgcg
     2641 ccggccaccg gcttgggccc gatcatgacg tcgcaggtgc tcgtgcccat gatcttgacg
     2701 agggtccctg gcgctatatc gccgccgacc gagccgatat gggcgtcgaa ggcgccgaca
     2761 ccgacgacga cgtcggtcga cagcccgagc cgcccggccc attccgggca gagcctgccg
     2821 gcggcttcct ccgcggtgta ggtctccgta cccaggctag cggctagctc ggagaggcgg
     2881 gggtcgaggc ggtcgaagaa ggcggcgctc gggtagcctc cccaggacgg gtgccacatg
     2941 gccttgtgtc ccatcgcgca tctgccgcgc cgaatgtccg aggcccgcgt gacgccggtc
     3001 agcagggagg gcatccagtc gcagtgctct atcacggtgg ccgcaacccg ggcggcctta
     3061 gccccgtcct cgacgaggtg gagcgccttg gcccagaacc actcggacga gtagatgccg
     3121 ccgatgtact tggtgtagtc ctcgccgccc caggtcctgg ccaccgcgtt gatccgctcc
     3181 gcctggcgca ccgacgtgtg atccttccag agcatgaaca tggcgtccgg atcgtccgag
     3241 aactccggcc acagcgcgag gggagagccc gactcgtcgg cgaggcaggg cgtggagccg
     3301 gtggtgtcga cggtgaggcc gcgtaccctg gacgccgcgc cggctccggc gttcgttagc
     3361 gctgctacca cgcattcctc gaggctctcg acgtagtcga ggggatgctg gcggaaacgg
     3421 ttcgccttgg ggtcgcagta catgcccttg gcccaccgtc gataggagct aaccgcggtg
     3481 ccgagcggct cgccgtcggc cgcgtcgacg accagggccc ggaccgagtc cgacccgaag
     3541 tctatgccga tgacgtaatc ccggcctttt ttgttagtca tctcgagcct cctcgcggtc
     3601 ccagtcgccg gaagggcggc ggcccggatc gcgtattcta ttctcccagc gggatattct
     3661 ctctaaagaa cagttcgagc ggtatctccg ccggacggcg cgacgccagc gccccttcac
     3721 cgagcaccac cgcgtggaag agaagctcga gcgcctcgcg cgcttgctcg gccgggcgct
     3781 gcgacacgag gcagtcgatc gagccgtcgg cgagggcccg cacgttatcc ggtacggcgt
     3841 cccatgagac gagcgatagc ttggttttga gccccctggc cgcgagccag tccccgacga
     3901 ggtggcccga ggaattggcc acgaggatgc cggcgatatc cgcggcgttt ccgaagatcc
     3961 tctcgagcgc tccctccttg gccgagctac cgtcgagtcc ggggcaatcg acggcgtcga
     4021 tgcgccgttc cgagtggtgg tcgctgaaga aggatcggaa cccgtcgatg cgccgggcga
     4081 tatgcctatc ctcggcatgc gccgagaggg ccacgagccg gccggcgccc ggcgacagga
     4141 gcgacatgac gcgcccggct aggtagccgc ctcggaacgg gtcctgggcc acgacggcga
     4201 gcggcgaggc gcactcgagg atgccgtcga agaagacgta gggcgtcgag ccgggctcgt
     4261 cccgggttcc atcgcccccg gcgcggcttc cgagcctggc cagggctccg cagagtatct
     4321 ccggcatggc gggagcgagg aggaggccgt cgcagggatc ctcgaggacg ctctcgagca
     4381 atacccggta ggccgcctcg tcgtagcggt cgaactcgtc gacgcgcacg tcgcagcggt
     4441 atcgggcgag gtcgagctcg gctcgcctga tgccctggag gcagagcgcc cagtaacccg
     4501 aatcctgatc cgatcgcggc aggatcacgc ggaaggtgaa gttcttcttg agcgatagct
     4561 gcctcgcgag gacgtcgggc tcgtagccga gcgacgatat gatctcgcgt atcctcgcct
     4621 gcgtcttgga ggagacgccg ccccggtcgt ggagtacgcg gtcgacggtg cctatggaca
     4681 ccccagcgag gcgggctatc tcctttacgg tgatcttttc ttccatgtct ctatctatcc
     4741 gcgtttgccg cttggcgtca gccatgcggg tagggcgcgc cgtaggccgc gcgctccgcg
     4801 tagccagagg acgacgctct gcagcacgat gaagatgcac aggagcagcc cgctcacgat
     4861 ggactggaaa ttcgagttga cgccggcggc gcgtatgagc tcgttgatcg tgagcagggt
     4921 cagcgccccg atgggcgagc ctatcaggtt cccgacgccg ccagagagca tcgtaccccc
     4981 gatgatcgaa ccggctatcg ccttcatctc gaacccggcc gcgttcccga tgttccccgc
     5041 tcccgtcgtc attatgaaga cgaagcccgc gacgccggag agtagcccgc tgagcacgta
     5101 ggagccgaat ttggcccgct tcacgttgat gccgagcatc agcgcgctct ggctattgcc
     5161 accgacggcg tagaggttcc gcccgaagcg cgaccatcgc atcagtacgg cgagcagggc
     5221 gatgagtgcc accgcgacga cggtgcccgg cttgatcgcg agcgggatga agttgccgag
     5281 ccggttcgtg gtgccgagcc acgggacgac gagctcgaag tccctgagga gcacgaactc
     5341 cggcagggtc acgttgagag gatccttgtg tatggtcgtc aggagcccgt tggccaggaa
     5401 catgcccgat agcgtaatga tgaacggctg gatttccagg tacgctacga gaaagccttg
     5461 gaggaggccg aacgcgaggc ctatgcccag cgcgaggagc atcgagcctc cgacgctccc
     5521 cgtcccggat tccaagaaaa ccgcgcagca catggttacg aggccggtca cggcgccgac
     5581 cgagatgtcg atgccgccgc ctatcatgac gatgctcagg ccgagggt
//
LOCUS       DAOPHV010000102         5604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_19064_length_5604_cov_4.305821, whole genome shotgun sequence.
ACCESSION   DAOPHV010000102 DAOPHV010000000
VERSION     DAOPHV010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19064_length_5604_cov_4.305821"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2795
                     /locus_tag="PLQ29_03995"
     CDS             <1..2795
                     /locus_tag="PLQ29_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85839.1"
                     /translation="LEDFGSLLETEEATEAASTDDAPTEEPTEPIEPIEPIEPIESLE
                     ATESETTGLDDFTVPDSLLDGAADETSQERPEMTGEAVRANDDFSFDDLESLESEGDS
                     EAAKPDDGFSIPAFDDMGLAEAEPEAPGSGEAAAPGPDPEARGAESAGFDLGSESMEG
                     DSFDQFDLGGGSPEVAVPEMGDSDFGGEFSDLGSESAAADTFSLGGDWGGDFTIPGFE
                     MGNEVKPTTAKPAASKEARPGKAPSSSLAGAFGSGEARVSAERKARPVELTENQADAL
                     QDTLLSYPLNLRLAIEDIVANQKGTELQQSDLVWLLVERASAKDAAKLAGKVLKRYIE
                     VPASFAKRTGAAFEAEKGSFRYLLVHSILPVLQVLLLVAAGAGAAFYLGYNFAYKPIK
                     AYSLYAEGHRQIGLSQFGESLELFERAEDEWSMKGWHYRYAEAYADAGQYPRAEAMYE
                     RLLARWPKEKRAAKDYATMEMDLLSFDTAEKVLQTYILSRDYFDKDALLLSARNLLAW
                     ADFEEQGYDPADPAEVARLYEKARLQLATLMERHGRSDGYLELMLIYLMRTERASGSD
                     KSEDALPLAKYFVENNRSAWAASTLAELAEYLLDRDETEYVNRILLSAVDRDGTVPET
                     HAAMARWNRRSGFPKDELKALEFAARFYAEADARSGLAPKRVKGYIDSMMRLGELRRQ
                     DGRGLDAEEAFNIAIDRYERALTSRQLRRDARFGRAYSLLADIYYAERMDFDGALELY
                     AKAEVNGFTSPQTDYRRGYIHYLSPKDDGSAALRFFYRAGLDLDASPYLQWATANALY
                     QRDDYFAAQGYLSTLANRLQFELDTITLPSPQTKPSHSEVVELLMMTRNNLGASLYRV
                     AERMGDTKRRAEAMVEFTESAKLYDSLARDQARMVRPESKNLGFLNLDFILHPQRGMD
                     LVIYKAIPVEMDYPRK"
     gene            complement(2954..3028)
                     /locus_tag="PLQ29_04000"
     tRNA            complement(2954..3028)
                     /locus_tag="PLQ29_04000"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2994..2996),aa:Val,seq:cac)
     gene            3470..4102
                     /locus_tag="PLQ29_04005"
     CDS             3470..4102
                     /locus_tag="PLQ29_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPG85840.1"
                     /translation="MSDVLRTNNTITIARPWSSEMRTELVRKSIHLLIGLVPTLASWN
                     FGVTVASLSAGILVYAYSESMRLHGYEVAVISKITAIAARRRDSGKFVLGPVTLGIGA
                     LLALVLYPEPAASIAIYALAFGDGLSSLVGKAFGSIRIPFTGGKSVEGSLTCFLAVLC
                     SAYAVTGDAPRSALIAGIATVTEALPSKDADNILLPTVVGLAAVFIFGLA"
     gene            complement(4125..4928)
                     /locus_tag="PLQ29_04010"
     CDS             complement(4125..4928)
                     /locus_tag="PLQ29_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85841.1"
                     /translation="MTIRLRERLYRAFFAAAASATILAVVAIWIPGLDGLERVRATAF
                     PSLDAVGFYSALAASGICAVYAAVVSGLIVFRSGRTVSVEIFFFSLWAFCQVFELARI
                     GSALLGMANANIGAYSLMTRFALFGRYSGSIGLFAGSLFSVGLKQERALPSMTFAFMA
                     GLLFASIHPLNSVAPGADLLADRGIEPLARSFELAILALALIDYCLAWRSVKDKAYLL
                     AAAGLTMCVVATTTLRAMATPWAVIAATPILVAGTWLYIKSMHDYYLWR"
     gene            5035..>5604
                     /locus_tag="PLQ29_04015"
     CDS             5035..>5604
                     /locus_tag="PLQ29_04015"
                     /inference="COORDINATES: protein motif:HMM:NF021529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2259 domain-containing protein"
                     /protein_id="HPG85842.1"
                     /translation="MKRWIAIVVLMLAFVSAAFAGDVATLVNLGFSPDSAYFMFGFYG
                     LNVANGKPYAEVYLVDTAKNEFVPGGMLKGMYGAELKPGWDPAGGFYKLFSDATPIAR
                     KHRIDHLAQGRLVYLLINGEDGPDVLSFKDFDTGIQWDVALKETVEDKAGAISSSFWL
                     EVSVESGSKKSSIRAGNPTVKRKGVTNYTI"
BASE COUNT         1151 a         1756 c         1703 g          994 t
ORIGIN      
        1 tgctcgagga tttcggttcg ttgctcgaga cggaagaggc taccgaagcg gcctcgacgg
       61 acgacgctcc tacggaggaa ccgactgagc cgatagaacc catcgagccg atagaaccca
      121 tcgagtccct cgaggcgacc gagtcggaga cgacggggct agacgatttc acggtaccgg
      181 attcgctcct ggacggtgcc gccgatgaaa cgtcgcaaga gcggcccgag atgaccggcg
      241 aggccgtaag agcgaacgat gatttcagct tcgacgatct ggagtcgctc gagagcgaag
      301 gcgattcgga ggcggccaag cccgacgacg gtttctccat accggccttc gacgacatgg
      361 gcctggcgga ggccgaaccg gaagcgcctg ggtccggcga ggcggcagca cccggccctg
      421 atcccgaggc gcggggtgcc gagtccgcgg gcttcgatct cggctccgaa tccatggaag
      481 gggactcctt cgaccagttc gacctgggcg gagggtctcc cgaagtcgcc gtaccggaga
      541 tgggcgatag cgacttcggc ggcgaattct ccgacctggg gagcgagtcg gcggcggccg
      601 acaccttctc cctgggcggc gactggggtg gcgactttac catccccggc ttcgagatgg
      661 gcaacgaggt caagccgacg accgccaagc ccgcggcgag caaggaagcg cggcccggca
      721 aagccccttc gtcatccctg gccggcgcct tcggttccgg cgaggccagg gtctccgccg
      781 aaaggaaggc ccgcccggta gagctgaccg agaaccaagc ggacgcgctc caggatacgc
      841 tcctatccta tccgctcaac cttcggctag ccatagagga cattgtcgcc aaccagaagg
      901 gcaccgagct ccaacagtcc gacttggtct ggctcctcgt cgagcgcgcc agcgccaagg
      961 atgcggcgaa actggccggc aaggtactca agcgctatat cgaggttccg gctagcttcg
     1021 ccaagcgtac cggcgcagcc ttcgaggccg agaagggcag ctttcgctac cttctggtac
     1081 attcgatcct gccggtgcta caggtactcc tactcgtagc ggccggggcc ggcgcggcct
     1141 tctaccttgg ctacaacttc gcgtataagc ctatcaaggc ctactcgctc tacgccgaag
     1201 ggcacaggca aataggatta tctcaattcg gagaatcgct cgagctgttc gagcgggccg
     1261 aggacgaatg gtcgatgaag ggctggcatt accgctatgc cgaggcctac gccgacgccg
     1321 gtcagtaccc gcgggccgag gccatgtacg agcggctgct cgccagatgg cccaaggaaa
     1381 agcgggcggc caaggactac gcgacgatgg agatggacct cctgtccttc gataccgccg
     1441 agaaagtgct acagacctac atcctgagcc gcgactactt cgacaaggac gcgttgctgc
     1501 tatcagcgcg caacctattg gcttgggccg acttcgagga gcagggctac gacccggccg
     1561 atccggccga agtcgcgagg ctgtacgaga aagcgcggct acagctggcc acgctcatgg
     1621 aacggcacgg ccgctccgac ggttacctcg agctgatgct gatctatctc atgcgtacgg
     1681 agcgcgcgag cggctcggat aagtccgagg acgcgcttcc cctggccaag tacttcgtcg
     1741 agaacaatcg gagcgcctgg gccgcctcga ccctggctga actggccgag tacctcctcg
     1801 acagggacga gaccgagtac gtcaaccgca tactgctatc ggccgtcgac cgcgacggta
     1861 cagtgcccga gacgcacgcg gccatggctc gctggaaccg caggtcgggt ttcccgaagg
     1921 acgagctcaa ggcgctcgaa ttcgccgccc gcttctacgc cgaggctgac gcacgttccg
     1981 ggctggcgcc gaagcgcgtt aagggctaca tcgactccat gatgcgcctc ggggagctac
     2041 gcaggcagga cggccgcggc ttggacgccg aggaagcctt caatatagcg atagaccgct
     2101 acgagcgagc cttgacctcg aggcaactcc gccgcgacgc gcgcttcgga cgggcctaca
     2161 gcctgctcgc cgatatatac tacgctgaac gaatggactt cgacggcgcg ctcgagctgt
     2221 acgccaaggc cgaggtgaac ggctttacca gtccgcagac ggactaccgg cgcggctaca
     2281 ttcattacct atcgcccaag gacgacggaa gcgcggctct gaggttcttc tatcgcgccg
     2341 gactcgacct cgacgcttcg ccgtacctgc aatgggccac cgccaacgcg ctctatcagc
     2401 gcgacgacta tttcgccgcc cagggctacc tatcgaccct cgctaacagg ctgcagttcg
     2461 agctcgatac cattacctta cccagccctc agaccaagcc gtcgcatagc gaggtcgtag
     2521 agctcctgat gatgacgagg aacaacctcg gcgcgagcct gtaccgggtt gccgagcgca
     2581 tgggcgacac gaagcgacgc gccgaggcga tggtcgagtt taccgagtcg gccaagctct
     2641 acgattccct ggcgcgcgac caagcgcgca tggtgaggcc ggagtcgaag aatctagggt
     2701 tcctcaacct ggacttcatc cttcaccctc aacgaggcat ggacctggtg atctacaagg
     2761 ctatacccgt cgagatggat tatccgagga agtagcgctt aacggctgta ctcggacgct
     2821 gaaccagtga cttcgacgct cgcgttcgct cgctaacgcg caaggtattg gtattgtagt
     2881 aattcgcctt cgactaatca tcgcgtaccg tgtgaaggtt cggcaagcgc aacccacaat
     2941 aaaaaggcgt ttttggtggg cgatactggg attgaaccag tgacttctac cgtgtgaagg
     3001 tagcactctc ccgctgagtt aatcgcccac caaaaacgcc atatgtaggg agggttgcgc
     3061 ttgccgcgca ctctcccgct gagttaatcg cccatagaac gcactatgta gagaaggtac
     3121 gcttcatgcg cacgctcccg ccgatcatag aagtatcaaa cagagcctgc tccataacgc
     3181 cttgcgcgat cgaggagcgc tcagatagta gctttgacgg ccggtgcatg tcaacatctt
     3241 cgtagccttg gctccagagg atagcgacac cgttccatcg ctttgaatag cgcattgacg
     3301 acactgagtg tattaattat tagacgatga acgattcaaa ggcgcaacgg agaacaggta
     3361 cactcgacta tttatagact caactatgat ttatttttcc cccacagcaa tactatattt
     3421 tggatcgctt cttgcatatt atagcgtaga acgatttgca aggatgcgta tgtccgacgt
     3481 actgagaacc aataacacca ttaccatagc tcgtccgtgg tcttcggaga tgaggacgga
     3541 gctcgtacgc aagagcatcc acttgttgat cggcctcgtc cctaccctgg cctcgtggaa
     3601 tttcggcgtg acagtcgcgt cgctatcggc cggcatcctg gtctacgcgt atagcgaatc
     3661 gatgcgtctt cacggctacg aagttgcggt catcagcaag atcaccgcca tagccgcccg
     3721 caggcgggac tcgggcaaat tcgtgctcgg ccccgtgacc ctcggcatcg gcgccctact
     3781 ggccctggtg ctctatcccg agccggccgc ctcgatagcg atctatgcgt tggccttcgg
     3841 cgacggccta tccagcctgg taggcaaggc cttcggctcg atacgtatcc cctttaccgg
     3901 cggcaagtcg gtcgaaggta gccttacctg cttcctcgcg gtcctgtgtt cggcctacgc
     3961 cgtcaccggc gacgctcccc gttcagcact gatcgcaggt atcgcgaccg ttaccgaagc
     4021 gctcccctcc aaggacgccg acaacattct gttgccgacc gtcgtcgggc tcgccgccgt
     4081 cttcatcttc ggcctagcct aggtcgagcc agccctcaga cggcctatcg ccacaggtag
     4141 taatcgtgca tgctcttgat atatagccac gtcccggcga cgagtatcgg ggtagccgcg
     4201 atcacggccc acggcgtcgc catcgcacgc agcgtcgtgg tggcgacgac gcacatagtc
     4261 agcccggccg cggccaggag atacgcctta tccttgaccg agcgccaagc gagacagtaa
     4321 tcgatcaatg ccagcgccag gatcgccagc tcgaagctgc gggcgagcgg ctcgatcccc
     4381 ctgtcggcca ggaggtcggc gcccggggcg accgagttga ggggatggat cgaggcgaac
     4441 aagagccccg ccatgaaggc gaaggtcatc gacggcaagg cgcgctcctg cttcaggccg
     4501 acggagaaca ggcttccggc gaacagcccg atcgaaccgg agtaccggcc gaacagggcg
     4561 aacctcgtca tcaggctata cgcgccaata ttcgcgttgg ccatgcctag gagcgccgag
     4621 ccgatcctcg cgagctcgaa gacctgacag aaggcccaga gcgagaagaa gaatatctcg
     4681 acggagacgg tcctcccgct ccggaagacg atgaggccgg agacgaccgc cgcgtagacg
     4741 gcgcagatac cggaggcggc caacgccgaa tagaagccga cggcgtcgag cgatgggaac
     4801 gcggtggccc ggacccgttc caggccgtcc agccccggta tccagatcgc caccacggct
     4861 agaatagtcg ccgaggcggc cgccgcgaag aacgcgcggt ataatcgttc cctgagtcgt
     4921 atcgtcatgc gactacccta ctaccagaag gcttttctct caaggccgat cgattcgagc
     4981 cgatacttgt agggagggtg accgccccga aggccacccg gggggagaca atcgatgaag
     5041 agatggatag ctatcgtcgt cttgatgctg gcgttcgttt cggccgcctt cgccggggac
     5101 gtcgcgacgc tcgtcaacct cggcttctct cccgattcgg cctatttcat gttcggcttc
     5161 tacggactga acgtggcgaa cggcaagccg tacgccgagg tgtacctggt cgatacggcc
     5221 aagaacgaat tcgtcccagg cggcatgctc aagggcatgt acggcgccga gctcaaaccg
     5281 ggctgggatc ccgccggcgg tttctacaag ctattctccg acgcgacccc gatagccagg
     5341 aagcaccgca tcgaccactt ggcccagggc cggctggtct acctgctgat caacggagag
     5401 gacgggcccg acgtcctgtc gttcaaggac ttcgataccg gcatccagtg ggacgtggcg
     5461 ctcaaggaga ccgtcgagga taaggccggc gcgatctcct cgtcgttctg gctggaggtc
     5521 tcggtggagt cggggtcgaa gaagtcgtcc atcagggccg gcaatccgac ggtcaagcgc
     5581 aagggcgtca ccaactacac catc
//
LOCUS       DAOPHV010000103         5604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_19065_length_5604_cov_3.815823, whole genome shotgun sequence.
ACCESSION   DAOPHV010000103 DAOPHV010000000
VERSION     DAOPHV010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19065_length_5604_cov_3.815823"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..586
                     /locus_tag="PLQ29_04020"
     CDS             35..586
                     /locus_tag="PLQ29_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPG85843.1"
                     /translation="MKKACVILANGCEEVEAITPIDYLRRAGVEVVAAGLDGREAVGA
                     HGLIIGADAVLDDIDDEEFDCIVVPGGGGGARAIAGSMVAIAMLKRQAVAGKPIAAIC
                     AAPALVLGEACGLLAGRRFTCYPGLEGRVPEGRFEPSRVVVDRELITARGPGCAGEFA
                     VAIARALVGDEKADELADGVLLS"
     gene            complement(583..972)
                     /locus_tag="PLQ29_04025"
     CDS             complement(583..972)
                     /locus_tag="PLQ29_04025"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HPG85844.1"
                     /translation="MTKVDGSDVTVRISGGEGCASCTAGHDCSIVGKELVGDAAAGYA
                     FAAGDEVELLVPDSASAAGALWLLAVPLGLFFAGYAAAGALWPGRGEGLRALLGLGAM
                     ALGLLFAAVVARRGRMSRRPTVVPLVA"
     gene            complement(959..1786)
                     /locus_tag="PLQ29_04030"
     CDS             complement(959..1786)
                     /locus_tag="PLQ29_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPG85845.1"
                     /translation="MGIRILYIGEIVGRAGVFAVKRLLPTLRRETAADLVVACANGAT
                     GGSGIGKAHSVYLRKLGIDVLTTGEAAFYKKDIVEAFPKSPWLLRPLNYPPGVPGRGF
                     RAYQTPKGPVVVAQLLGQAGFPRVHLENPFHVLDAALPTLSEGSKAVLLDFRAATTAE
                     RRAMARYADGRVSAVLGSYGRALSADAEVSAAGTASMTDTGRTGSLMSVGGMDAATRI
                     HEYRSGVPVWAKDSSEAPELQGCVIELGADGRAVSIETLRVPCEEEFVEGTRHSDQG"
     gene            complement(1834..2325)
                     /locus_tag="PLQ29_04035"
     CDS             complement(1834..2325)
                     /locus_tag="PLQ29_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HPG85846.1"
                     /translation="MPEIAYSRPTWDEYFMEVCRAIAKRATCDRGRSGCVIAKDNQIL
                     ATGYVGSPSGLPHCDELGHQFRKTVHKDGSESTHCVRSVHAEQNAICQAARRGIAIEG
                     ATLYCKMTPCRACAMMIINCGVVRVVCERKYHAGAESEEMFSKVGILVEYVEDEVERY
                     ERQ"
     assembly_gap    2357..2456
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2561..3583
                     /locus_tag="PLQ29_04040"
     CDS             2561..3583
                     /locus_tag="PLQ29_04040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPG85847.1"
                     /translation="MRFIKTGAMAAVLLAFASAVSCQSGPKLPDGLYAVIDTVKGSLT
                     ISLDYERAPLAVANFVGLAEGTLDSNAGKRFYDGLSFHRVEPGFVVQGGDPLGDGSGD
                     PGYNFPDEFDPAVRHDAPGVVAMANHGPDTNGSQFYITLDAAPFLDDSYTAFGRVVDG
                     MDVVQRIAAGDVMTKVTIRRVGDAAKAFETDQAAWNRYYGPAAEASKLRALASRQTSV
                     DRILEKWPGLERQPTGILSKVLKEGSGPTMRRGYLVNVSYKGMLPDGRVFDQSILHGG
                     PIEFELGTGQVIVGWDLTIEQMRKGERRLVAIPPELAYGTQGAGGVIPPNSYLVFELE
                     VTGYTE"
     gene            complement(3653..4594)
                     /gene="ricT"
                     /locus_tag="PLQ29_04045"
     CDS             complement(3653..4594)
                     /gene="ricT"
                     /locus_tag="PLQ29_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPG85848.1"
                     /translation="MDDDMDYMQGDEDLSHLEDDAVPAADAGVHCGELPDPLYRLRAD
                     PYNESYYACFDTPLEPGARVVAPTRYGKDICTVLGTVKAPGFVRVDDIVRIDRVSTPE
                     DEARRVADEQKEKKAFELCQEKIKAHNLPMKLVFAHYLFDEQKVLFFFTADSRVDFRN
                     LVKDLVSIFKMRIELRQIGVRDEARVVGGCGVCGRVLCCHSVTDKLVPVSIKMAKDQN
                     LSLNSLKISGPCGRLLCCLAYEYGFYREARRGMPSEGVRIPYDGTVFRVVEVNVPAGR
                     LRLSGEDGRYVEAPSSRFVQRDGRWAMLDEEAAESAD"
     gene            complement(4627..5130)
                     /locus_tag="PLQ29_04050"
     CDS             complement(4627..5130)
                     /locus_tag="PLQ29_04050"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF327 family protein"
                     /protein_id="HPG85849.1"
                     /translation="MEKVGLPDNLISSLVPGLSGQRADKKKEATKASPKALRFRSDLK
                     EAEEIDRTPAAEGLPFSEAEAAELLDAVHASGEELKRDPDPARVKAYKAAVRDFVHYV
                     VGRAYDVVEKSSGGNILKRKKFTTVVVIDEKLERLAAEVLSAQRDKLDILRRLDEIHG
                     LLVDLLR"
     gene            complement(5118..5588)
                     /locus_tag="PLQ29_04055"
     CDS             complement(5118..5588)
                     /locus_tag="PLQ29_04055"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPG85850.1"
                     /translation="MPAPRDVLVNTLLGPDSSFRGDLVVDGFIRVDGDVRGTIRSSGK
                     IVISQAARCDASIVARSAVVGGVVRGDVCVTERLTILDGGVIVGNVFTPRLDADGEIL
                     IHGDVQVAPSGEGVEEAMLTFIRRHGSTIRPFGFDAPSDERRGEAVSSGAAWKR"
BASE COUNT          924 a         1861 c         1831 g          888 t
ORIGIN      
        1 tgatcgaggc ccggacctcg ccgagcagga gagcatgaag aaggcttgcg tgattctggc
       61 taacggctgc gaggaagtcg aggctatcac cccgatcgac tacctacgta gggccggcgt
      121 cgaggtcgtg gcggccgggc tggacggccg cgaggccgtc ggggcgcacg gcctgatcat
      181 cggggccgac gccgtactcg acgacatcga cgatgaggag ttcgactgca tcgtcgtgcc
      241 gggcggcggg ggcggggccc gggccatagc cggaagcatg gtggcgatcg ccatgctcaa
      301 gcgccaggct gtcgccggca agccgatagc ggcgatctgc gccgccccgg ccctggtgct
      361 cggcgaggcc tgcgggctac tggccgggcg ccgttttacc tgctatcccg ggctcgaagg
      421 ccgggtcccc gagggccggt tcgagccaag ccgcgtcgtc gtcgaccggg agctcataac
      481 ggccagggga ccgggctgcg ccggggaatt cgccgtagcg atagccaggg ccctcgtcgg
      541 cgacgaaaag gccgacgagc tggccgacgg cgtacttttg tcctaggcga cgagggggac
      601 gaccgtcggc cgcctggaca tgcggccgcg gcgcgccacg acggcggcga acaggaggcc
      661 gagggccatc gccccgagcc cgagcagcgc tcggagcccc tcgccgcggc caggccacag
      721 cgcgcccgcc gcggcgtagc cggcgaagaa cagcccgagc ggcaccgcca acagccagag
      781 cgccccggcc gcgctggccg aatccggcac cagcagctcc acctcgtcgc ccgcggcgaa
      841 ggcgtaaccc gcggcggcgt cgccgaccag ttccttgccg acgatcgagc aatcatggcc
      901 ggcggtgcag gacgcgcagc cctctccgcc ggaaatacgg acggtgacgt cgctaccgtc
      961 aaccttggtc actatgcctc gttccctcaa cgaattcctc ctcgcacggc acgcgtagcg
     1021 tctcgatcga cacggcgcgc ccgtccgctc ctagctcgat gacgcagccc tgaagctcgg
     1081 gtgcctccga cgagtccttg gcccagacgg gtaccccgct ccggtactca tgaatcctcg
     1141 tagcggcgtc catgcccccg actgacatca ggctgccggt ccggccggta tcggtcatgc
     1201 tggcggtacc ggctgccgac acctcggcgt cggcgctcaa cgcgcggcca taggagccga
     1261 gcacggcgct gaccctgccg tccgcgtacc tggccattgc ccggcgctcc gcggtcgtgg
     1321 cggcgcggaa gtcgagcagt accgccttcg atccctccga cagggtcggc agagcggcgt
     1381 cgagcacatg gaacgggttc tcgaggtgta cccgcgggaa gcccgcctgg ccgagcagct
     1441 gcgcgacgac gaccgggccc ttgggcgtct ggtaggcccg gaagcccctg ccaggcacgc
     1501 cgggcgggta gttcagcggc cgcagcagcc atggcgactt cggaaaagcc tcgacgatat
     1561 ccttcttgta gaacgccgct tcgccggtgg tcagcacgtc tatgcccagc ttgcgcaggt
     1621 agaccgaatg cgccttgcct atgccggaac caccggtggc gccgttggca cacgcgacga
     1681 ccaagtcggc ggcagtctct cgcctgagcg tcggcaggag tcttttgacg gcgaagacgc
     1741 cggcgcggcc gactatctcg cctatgtaca ggatgcgtat acccacgcgg acaaggtacc
     1801 tgaaacggta ccggggttca agccggaacg cgcctactgg cgctcgtagc gctccacctc
     1861 gtcctcgacg tactccacga gtatcccgac cttcgagaac atctcctcgc tctcggcgcc
     1921 ggcgtggtac ttgcgttcgc agaccacgcg gacgacgccg cagttgatga tcatcatcgc
     1981 gcaggcgcgg catggcgtca tcttgcagta cagcgtggcg ccctcgatgg ctatgcccct
     2041 acgagcggcc tgacagatgg cgttctgctc cgcatggacg ctacgcacgc aatgagtgga
     2101 ctcggagccg tccttgtgga cggttttcct gaactgatgg ccaagctcgt cgcaatgggg
     2161 caggcccgat ggagaaccga cgtagccggt ggccaggatc tggttgtcct tggcgatcac
     2221 gcagcctgag cgccctcggt cgcaggtggc gcgcttcgcg atcgcacggc agacctccat
     2281 aaaatactcg tcccaggtcg gcctcgagta cgcgatttca ggcatgggtt cctccacgga
     2341 gcattatacg gccgcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcgc
     2461 gacgaacgga gtgaggcgcg acgaacggag tgaggcgatg aaccttgccc ccgcggccgt
     2521 tttatggtag cgtcgtcacg ttcattccgg aggtacatcg atgcgcttca tcaagaccgg
     2581 cgccatggcg gcggttctcc tggctttcgc ctcggccgta tcatgccagt cgggccccaa
     2641 gctacccgac ggcctctacg ccgtgatcga tacggtcaaa ggatcgctca cgatatcgct
     2701 cgactacgag cgcgcccccc tcgcggtcgc aaatttcgtc ggcttggccg agggtaccct
     2761 cgattcgaac gccggcaagc gcttctacga cggcctgtcg ttccaccgcg tggaaccagg
     2821 cttcgtggta cagggcggcg acccgctcgg cgacggctcg ggcgacccgg gctacaactt
     2881 cccggacgag ttcgacccag ccgtccggca tgacgcgccc ggcgtcgtcg cgatggccaa
     2941 ccacgggccg gacacgaacg gcagccagtt ctacataacg ctcgacgccg ccccctttct
     3001 cgacgactcg tataccgcct ttggccgagt cgtcgacggg atggacgtcg tccagcggat
     3061 agccgcaggc gacgtcatga ccaaggtgac gatacgcagg gtcggcgacg cggcgaaggc
     3121 cttcgaaacc gaccaggccg cctggaaccg ctactacgga ccggcggcgg aggcctcgaa
     3181 gctgcgcgcc ctggcctcga ggcagaccag cgtggacagg atactcgaga agtggccggg
     3241 cctcgagcgg cagcccacgg gcatcctttc gaaggtgctc aaagaaggct ccggaccgac
     3301 gatgcgcagg ggctacctcg tcaatgtctc ctacaagggc atgcttccgg acggccgcgt
     3361 cttcgaccag tcgatcctac acggcgggcc gatcgagttc gagctgggca ccggccaggt
     3421 aatcgtaggc tgggacctga ccatcgagca gatgaggaag ggcgagcgcc gcctggtggc
     3481 cataccaccg gagctggcct acggcaccca gggcgcaggc ggcgtcatcc ccccgaactc
     3541 gtacctggtc ttcgaactcg aggtgaccgg ctacaccgag tagcctgagc gacgatacga
     3601 cgaggccccg gaaccaaacg gatccggggc ctcgtcgcgt atacggcagg cgttagtccg
     3661 cggactcggc cgcctcctcg tcgagcatgg cccagcggcc gtcgcgctgc acgaagcggg
     3721 aggacggagc ctcgacgtag cggccgtcct ctcccgacaa gcgtagccgt cccgccggca
     3781 cattcacctc cacgaccctg aaaaccgtgc catcgtacgg gatcctgacg ccctcgctcg
     3841 gcatgccacg cctggcctcg cggtagaagc cgtactcgta ggcgaggcag cagagcaggc
     3901 gcccgcaggg gccggatatc ttgagcgagt tgagcgacag gttctggtcc ttggccatct
     3961 tgatcgacac gggcacgagc ttgtcggtga cgctgtgaca gcagagcacc cgcccgcaga
     4021 ccccgcagcc gccgacgacc cgcgcctcgt cgcgcacgcc gatctggcgt agctcgatac
     4081 gcatcttgaa aatggatacc agatccttga ccaggttgcg gaagtccacc ctcgagtcgg
     4141 cggtgaagaa gaacagcacc ttctgctcgt cgaacaggta gtgggcgaag acgagcttca
     4201 tcggcaggtt atgggccttg atcttctcct ggcacagctc gaaggctttt ttctccttct
     4261 gctcatcggc caccctgcga gcctcgtcct ctggcgtcga gacgcggtct atccggacga
     4321 tgtcgtcgac gcgcacgaag ccgggcgcct tgaccgtgcc gagcacggtg cagatgtcct
     4381 tgccgtagcg agtcggcgcg acgactcggg cgcccggctc gagaggggta tcgaagcagg
     4441 cgtagtacga ctcgttgtag ggatcggccc ggagccggta caggggatcg ggcagttcgc
     4501 cgcagtgaac gcccgcgtcc gcggccggta cggcgtcgtc ttcgaggtgg ctgaggtcct
     4561 cgtcgccctg catatagtcc atatcgtcat ccatgatcgt ctctcctagc ggccctgacg
     4621 gccgattcag cgcaggaggt ccacgagcaa gccgtggatc tcgtcgaggc gccgcagaat
     4681 gtcgagcttg tcgcgctggg ccgagagtac ctccgcggcc aggcgctcga gcttctcgtc
     4741 tatcacgacg acggtggtga acttcttccg tttcaggata ttgccgcccg aactcttctc
     4801 gaccacatcg taggcgcggc cgacgacgta gtggacgaag tcccgcaccg ccgccttgta
     4861 cgccttgaca cgggcggggt cgggatcgcg cttgagttct tcgccggagg cgtggaccgc
     4921 atcgagcagc tcggcggcct ccgcctcgga gaacggcagc ccctcggcgg ccggcgtacg
     4981 gtcgatctcc tcggcctctt tgaggtccga cctgaagcgc agggccttgg gagacgcctt
     5041 ggtggcctct ttcttcttat cggcgcgctg gcccgacagc ccgggaacga ggctcgagat
     5101 caggttgtcg gggaggccta ccttttccat gcggccccgg aggagacggc ctctcctcgg
     5161 cgctcgtccg aaggcgcgtc gaagccgaac ggcctgatcg tcgagccgtg tcggcggatg
     5221 aaggtgagca tggcctcctc gacgccctcg cccgatgggg cgacctggac atcgccgtgg
     5281 atcaggatct cgccgtcggc gtccagcctc ggggtgaaga cgttgccgac gatcacgccg
     5341 ccgtccagga tggtcaggcg ctcggtgacg cagacgtcgc cgcggacgac gccgccgacc
     5401 acggcggacc tggctacgat cgaagcgtcg caacgggccg cctggcttat cacgatcttg
     5461 cccgagctgc gtatggtgcc gcggacgtcg ccgtcgacgc gtatgaaacc gtcgacgacc
     5521 aggtcgccac ggaacgagct gtcggggccg aggagcgtat ttacgagaac gtcgcgaggg
     5581 gccggcatgc cagtatccta cccg
//
LOCUS       DAOPHV010000104         5589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_19151_length_5589_cov_4.641308, whole genome shotgun sequence.
ACCESSION   DAOPHV010000104 DAOPHV010000000
VERSION     DAOPHV010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19151_length_5589_cov_4.641308"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..93)
                     /locus_tag="PLQ29_04060"
     CDS             complement(<1..93)
                     /locus_tag="PLQ29_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HPG85851.1"
                     /translation="MMRSLYSGVAGLQNHQVRMDVLGNNIANVNT"
     gene            complement(106..540)
                     /gene="flgD"
                     /locus_tag="PLQ29_04065"
     CDS             complement(106..540)
                     /gene="flgD"
                     /locus_tag="PLQ29_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HPG85852.1"
                     /translation="MDIDLRMSQADAARTSYQVDALNKELSGGKAFKTELDKDDFLKI
                     LITQLQHQDPTNPMQDKEFIAQMAQFSSLEQMTNMATSFGKLSTVLNSSEAQSLLGRT
                     VEVLDGDKAIYGKVSQVVRGEFPLVMVNGSFYDLDQVSKVEE"
     gene            complement(556..1818)
                     /locus_tag="PLQ29_04070"
     CDS             complement(556..1818)
                     /locus_tag="PLQ29_04070"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HPG85853.1"
                     /translation="MLALTRTVTAQSGLGASPPKADSAKAASVQGNPFTRLLVAAERG
                     VARAGTNAAKADGAGASRGVARRAVSAPSGAGGSNAALRAPAPAEASAGAEAARAKAT
                     ATKRSAGDARGIAGKHPDEAPVSNVAAFQRRSVAGDEASARDGASALAAIAARDASGA
                     QAAKPTLVPGRDGSDQRRSGGENGSSGIETVGVGAERRADGAKVTVVDLRLKATRGAV
                     SRREAGNEADDRGGLASTAKPAGVADAANPGLALGEPVAETAGSAKQPIEAVVAPTPP
                     TAEGLASRLREGAADIVRSAQIVLRGGDSGVIRLRLEPESLGGVKIELKMAEKQISGK
                     IVVESDIAAEAFRSSLDSLKDAFAECGFETSALEVEVRNGMASGGDGRAGSERDDGPY
                     WSRSLRELDAAVPQLVGSGRDGLLDVVV"
     gene            complement(1880..2467)
                     /locus_tag="PLQ29_04075"
     CDS             complement(1880..2467)
                     /locus_tag="PLQ29_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlbB"
                     /protein_id="HPG85854.1"
                     /translation="MLIMILALAIGGLFWFDYLGIVDAKAFFAPALRLAGLRTRTGEA
                     LPSDSPTLLDDERFAKQLGAVEAMRQELSERERQAEERQAAVEAMAQEIDDRARELDE
                     REISFKQMAERYENRRANVEQNARYLTGMPPADAVRILAASDDQTVIDVLRAVEELAA
                     KSGEASVVAYWLSLLPAERSATLQRKMNEKPASLD"
     gene            complement(2537..2977)
                     /gene="fliJ"
                     /locus_tag="PLQ29_04080"
     CDS             complement(2537..2977)
                     /gene="fliJ"
                     /locus_tag="PLQ29_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HPG85855.1"
                     /translation="MRRFAFKLERVLELREYDEKVAEAVLGEKTAVCSRLTLALEANA
                     RAAVEAARERFRPGSDASDHRAGELYAVRLSQERERLFKSLAAAELEREAARVDYVEA
                     TKARRLVTKLREREEAVYYKAVAREETKAMDDLAAGAHARADAR"
     gene            complement(2974..4410)
                     /locus_tag="PLQ29_04085"
     CDS             complement(2974..4410)
                     /locus_tag="PLQ29_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HPG85856.1"
                     /translation="MELIAKYLDAVERSDPIKYTGRVVKVQGLLVESRGPQAVVGELC
                     QILIPRLGKVAWAEVAGLRGETVQLMSYSGLDGIEVGCSVIATGEMLRVPVSDKLLGR
                     VLDSMGRPSDGKGDIASPDFYPAVAIPPDAMTRKRIKTRIGSGVRSIDGLLPLGRGQR
                     IGIFAGSGVGKSTLLGMMARNTNADVNVIALIGERGREVREFIENDLGPEGLARSVLI
                     VSTSDTPPLARLRGAYVATAVAEYFRDRGLDVLLLFDSVTRFARAQREIGLAIGEPPT
                     TRGYTPSVFDSMPKLLERSGTSDKGSITGVYTILVDGDDMDEPIADTVRGILDGHVVL
                     SRKLAERYHYPAVDVLKSISRLSQAVTGPITQKAMGVVRRLMATYDEAEDMINIGAYV
                     KGTNPAIDEAIAKRAAIEEFLIQGVAEKAPIADTIKRLGDIAGLSVPASELTDFAEPA
                     RPIAPPRASRDDGSGGVSAAGPEAGAEP"
     gene            complement(4414..5331)
                     /gene="fliH"
                     /locus_tag="PLQ29_04090"
     CDS             complement(4414..5331)
                     /gene="fliH"
                     /locus_tag="PLQ29_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HPG85857.1"
                     /translation="MAKTVFRPQETVPLSSAYVLHAPGGDIVVEDVEAVAPALEYEGP
                     TADDLRAEAELFSQNWEREKEAMMAAARAEAQDIVAKAEAAAFEEVRRKNDQALKIRR
                     EAESAAEKAIAEAEAKIQAFEAQAKARVDEVTKGARKEGFDQGREEGFKEGKLEVERL
                     VDRLHVILDRAMDKRAEILEQTEAQVVELVLLIARKVVKTISENQKNVVLSNITQALR
                     KLKTRSDVIVRVNIADLQLTTEHAKDFIEAAETAKKLSIVEDSSVDRGGCVIETDFGE
                     IDARIQSQLHELEEKILDISPIKARGKAQ"
     gene            complement(5345..>5589)
                     /locus_tag="PLQ29_04095"
     CDS             complement(5345..>5589)
                     /locus_tag="PLQ29_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HPG85858.1"
                     /translation="VDTQDLTRALKGVDTEVQEKVFQNVSKRAAQALKEDMEYMGPVR
                     IKDVEEAQQKIVAVIRHLEDTGEIIIARGEDDQLIQ"
BASE COUNT          805 a         1929 c         1775 g         1080 t
ORIGIN      
        1 ggtgttgaca ttggcgatgt tattgccgag cacgtccatg cggacctggt ggttctggag
       61 gccggcgacg cctgaataca acgagcgcat cataacggcg cctccttact cttcgacttt
      121 actgacctgg tcgaggtcgt agaacgaacc attgaccatg acgagcggga attcgccgcg
      181 tacaacctgg gataccttgc cgtagatcgc cttatcgccg tcgagcacct cgacggtacg
      241 gccgagcaac gactgggctt cgctcgagtt gagcacagtc gacagcttgc cgaagctcgt
      301 cgccatgttg gtcatctgct cgagcgagga gaactgggcc atctgcgcga tgaactcctt
      361 gtcctgcatc gggttggttg gatcctggtg ctggagctgg gtaatcagga tcttcaggaa
      421 gtcgtccttg tccagctcgg tcttgaaagc cttcccgccg gacagttcct tgttcagggc
      481 gtccacctgg tagctggtcc tggccgcgtc cgcctggctc atccgtaggt ctatatccat
      541 cgatcgctcc ttactttaca ccactacgtc gaggaggccg tcccggcccg acccgaccaa
      601 ttgcggcacc gccgcgtcaa gttcgcgcag cgagcggctc caatagggac cgtcgtcgcg
      661 ctcgctcccg gcacgcccat cgccgccgga ggccatgccg ttccggacct ctacctccag
      721 cgcgctcgtc tcgaagccgc attcggcgaa cgcgtccttg agcgaatcga gcgacgacct
      781 gaaggcctcg gccgctatat ccgattctac gactatcttg ccgctgattt gtttttcggc
      841 cattttgagc tctatcttga cgcctccgag cgattcaggc tccagccgga gccgaatgac
      901 ccccgaatcg cctccgcgca ggactatctg agccgagcgg acgatatcag cggctccctc
      961 gcgcaacctg gacgccaggc cctcggccgt gggaggagtc ggagccacaa cggcttcaat
     1021 cggctgcttg gcgcttccag cggtttcagc gaccggctcg cccaacgcga ggcccgggtt
     1081 cgcggcatcg gctacgcccg ccggtttggc ggtcgaggcg agcccgccgc gatcgtcggc
     1141 ctcgttgccg gcctctcgcc tcgagacggc tcctcgggta gccttgagcc gcaggtccac
     1201 cacggttact ttggccccgt cagctcttcg ctcggcgccg acgccgacgg tctcgattcc
     1261 cgaggaaccg ttctcgccgc ccgagcgccg ctggtcggag ccatcccgcc ccggaaccag
     1321 ggtgggcttc gcggcttggg cgccggaagc gtcccgcgcg gcgatagccg ccagcgcgga
     1381 ggctccgtcg cgcgccgacg cctcgtcacc cgcgacggag cggcgctgaa aggcggcgac
     1441 gttcgagacg ggggcctcgt cgggatgctt cccggctatt ccgcgcgcat cgccggcgct
     1501 ccgtttcgta gcggtggcct tggctcgcgc ggcttcggct ccggcgctcg cctcggcggg
     1561 cgctggagct ctcagggcgg cattcgaccc gccggctccg gagggggcgg aaacggcccg
     1621 ccgggcaacg ccgcgggacg cgcctgcccc gtcggctttg gccgcgttgg tccctgccct
     1681 ggcgacgccg cgctcggccg cgaccagcag cctagtgaat ggattgcctt gtacgctcgc
     1741 cgccttagcg gagtcggcct tcgggggcga ggcccccagg ccgctctggg cggttacggt
     1801 acgtgttagc gccagcatgt atgcgggctc cttccgcgtc cggctaggcc gaaccgctcg
     1861 ggaggagtcg gctcgggcgc tagtccagcg acgctggttt ctcgttcatc ttgcgctgca
     1921 gcgtcgcgga acgctccgcg ggcaacagcg acagccaata cgccacgacg gaggcttctc
     1981 ccgacttggc ggccaactcc tccaccgctc tcagcacgtc gatgaccgtc tgatcgtccg
     2041 aggccgcgag tatcctcaca gcgtcggccg gcggcatgcc ggtcagatac ctggcgttct
     2101 gctcgacgtt cgctcttcta ttctcgtacc gttcggccat ttgtttgaat gaaatctcgc
     2161 gctcgtcgag ttccctggcc ctatcgtcga tttcctgagc catagcctcg accgcggcct
     2221 gcctctcctc ggcctgacgc tcgcgctcgg atagctcctg acgcatcgct tcgacggcgc
     2281 cgagctgctt cgcgaagcgc tcgtcgtcga gcagcgtcgg ggaatcggat ggtagcgcct
     2341 cgccggtacg cgtcctgagg ccggccagcc tcagggccgg ggcgaagaaa gccttggcgt
     2401 ccacgatccc gaggtaatcg aaccagaata agccacctat ggcgagggcg aggatcatga
     2461 tgagcatgac gactacccta ccgaaaacgc gcctcttacc gaacgatgcc atactgcccc
     2521 tctacgttcc agatgcctag cgtgcgtcgg cacgggcgtg cgccccggcg gccaggtcgt
     2581 ccatcgcctt cgtctcttcg cgggcgaccg ccttatagta cacggcttct tctcgctccc
     2641 tcaacttggt gacgaggcgg cgggccttgg tagcctcgac gtagtcgacg cgcgccgcct
     2701 cgcgctcgag ctcggcggcc gccaacgact tgaacagccg ctcgcgctcc tgcgacagcc
     2761 tgaccgcgta cagctcgccg gcccggtggt ccgacgcgtc gctacccggc ctgaagcgct
     2821 ccctggcggc ctccaccgcg gcgcgggcgt tagcctcgag cgccagggtc aggcgggagc
     2881 agacggccgt cttttcgccc aggacggcct ccgcgacctt ctcgtcatat tctcgcagct
     2941 caagcacgcg ctcgagcttg aacgcgaaac gcctcacggc tcggctccgg cctcgggacc
     3001 ggcggccgag acgcctccgg agccgtcatc gcggctggct ctcggtggcg cgatcggccg
     3061 cgccggctcc gcgaagtcgg tcagctcgct cgccggtacc gaaagcccgg cgatatcgcc
     3121 gagccgcttg atcgtgtccg ctatcggcgc cttctcggcg acgccctgga tcaggaactc
     3181 ctctatagcg gcgcgcttgg ctatggcctc gtctatcgcc gggttggtac ccttgacgta
     3241 cgcgccgata ttgatcatgt cctcggcctc gtcgtaggtg gccatcagcc gcctgaccac
     3301 gcccatcgcc ttctgggtga tcggcccggt gacggcctgg gacagcctcg agatggactt
     3361 gagtacgtcg acggcagggt aatggtatcg ctcggccagc ttgcgcgaca gcacgacgtg
     3421 gccgtcgagg atgccgcgga ccgtatcggc tatcggctcg tccatatcgt caccgtcgac
     3481 gagtatcgta tagacgcccg tgatcgagcc cttgtccgag gtgcccgagc gctcgagtag
     3541 cttgggcatc gagtcgaaga cgctcggagt atagcctcgc gtcgtcgggg gctctccgat
     3601 cgccagccct atctcgcgct gggccctggc gaagcgggtg accgagtcga acaggaggag
     3661 cacgtcgagg ccgcggtcgc ggaagtactc ggcgaccgcg gtagccacgt aggcgccgcg
     3721 gagcctggcc agaggcgggg tatccgaggt ggagactatg agcaccgagc gcgccaggcc
     3781 ctcggggccg aggtcgttct cgatgaattc gcggacctcg cggccgcgct cccctatgag
     3841 cgctatgacg ttgacgtcgg cgttcgtgtt gcgcgccatc atgccgagca gcgtcgactt
     3901 gccgactccg gagccggcga aaatgccgat gcgctggcca cggccgagcg gcaggaggcc
     3961 gtcgatcgag cgcacgcccg agccgatgcg cgtcttgatg cgcttacggg tcatcgcgtc
     4021 gggcgggatg gccaccgccg ggtagaaatc gggcgaggcg atgtcgccct tgccgtccga
     4081 cggtcggccc atcgagtcga gcacgcgccc gagcaacttg tccgacaccg gcacgcgtag
     4141 catttcgccg gtcgctatca cgctgcagcc cacctcgatg ccgtcgaggc cggaatacga
     4201 catcagctgt acggtctcgc ctcgcaggcc ggccacctcg gcccaggcca ccttgccgag
     4261 cctcggtatc agaatctggc atagctcgcc gacgacggcc tgcgggcctc ggctctcgac
     4321 gaggaggccc tgtaccttga cgacgcgacc ggtgtacttg atcggatccg agcgctcgac
     4381 cgcgtcgagg tacttggcta tgagttccac ggcctactgc gccttcccgc gcgccttgat
     4441 cggcgatatg tcgaggattt tctcctcgag ctcgtggagc tggctctgga tcctcgcgtc
     4501 tatctcgccg aaatcagtct ctatgacgca accgccgcgg tcgaccgatg aatcctcgac
     4561 gattgaaagc ttcttggctg tctcggccgc ctcgatgaag tccttggcgt gctcggtagt
     4621 caactggaga tcggctatat tgacgcgcac tatcacgtcg ctgcgggtct tgagcttgcg
     4681 cagggcctga gtgatgttcg agagtacgac gttcttctgg ttctcggaaa tggtcttgac
     4741 gaccttgcgg gctatgagca gcaccagctc gacgacctgg gcctcggtct gctcgagtat
     4801 ctcggcgcgc ttgtccatcg cccggtcgag tatcacgtgg agccggtcga cgaggcgttc
     4861 gacctcgagc ttgccctcct tgaacccttc ctctcggccc tggtcgaagc cctccttgcg
     4921 agcccccttg gtcacctcgt cgaccctggc cttggcctgg gcctcgaagg cctggatctt
     4981 ggcctcggcc tcggcgatcg ccttctcggc ggccgactcg gcctcgcggc gtatcttcag
     5041 ggcctggtcg ttcttgcgcc gaacctcctc gaaggcggcc gcctcggcct tggccacgat
     5101 gtcctgcgcc tccgcccggg cggcggccat catggcctcc ttctcgcgtt cccagttctg
     5161 gctgaacagc tcggcctcgg cccggagatc gtctgcggtc ggcccctcgt actcgagcgc
     5221 gggcgcgacg gcctccacgt cctcgacgac gatgtcgccg cctggggcgt ggagcacgta
     5281 ggcgctcgag aggggcacgg tctcctgcgg cctgaacacg gttttagcca tacgctacct
     5341 tttcctactg gatcagctga tcgtcctcgc cgcgggcgat gatgatctcg cccgtgtcct
     5401 cgaggtggcg gattaccgcg actatcttct gctgggcctc ttcaacgtcc ttgatgcgta
     5461 ccgggcccat gtactccatg tcctccttga gcgcctgcgc ggcgcgcttg gacacgttct
     5521 ggaacacctt ctcctgcacc tcggtgtcga cgcccttgag ggcgcgcgtg aggtcctggg
     5581 tgtcgacct
//
LOCUS       DAOPHV010000105         5573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_19241_length_5573_cov_4.185393, whole genome shotgun sequence.
ACCESSION   DAOPHV010000105 DAOPHV010000000
VERSION     DAOPHV010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19241_length_5573_cov_4.185393"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PLQ29_04100"
     CDS             <1..693
                     /locus_tag="PLQ29_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85859.1"
                     /translation="SALLRGEEPRASSSGADPALSLLPLHPAADLIRGAIRSEAPDVV
                     VEAVFLWRRRGPATADEVLLVYNALRSIGSLEGIQYYSASRGRTRTLYERSTLLAGPN
                     SDRALTDTRLQALPAGGETLYALQRDTTFGENRYRVELSAGDGYVAQSSSNLTGMRLG
                     LVPVAGPGDVVVRVLVIQVDEGLLFYVASSAKAAVVPGVRGSLERSFANRAAAAFAWF
                     SGIMDAAAPSGR"
     gene            complement(690..2345)
                     /locus_tag="PLQ29_04105"
     CDS             complement(690..2345)
                     /locus_tag="PLQ29_04105"
                     /inference="COORDINATES: protein motif:HMM:NF036553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HPG85860.1"
                     /translation="MGILQAILSFFRGLLGLEIGAKGELKRIYSVISPIKPPYYRFKN
                     NLVMTGFAQDLYLFCQAIKPLMDLADRTLAHPDLRVSRRYFDYLVDLELPPQELEKKE
                     SFAYDGMKARIENAVRDDEEQEAVAKDFQHFLKSLEALSDGPLNTDLHEVERFIEVCR
                     HDWERTLGFFDPGASLDDSRYRPDFQPCAGEQVLPELIDAYYLLIGFSFSENLLRKTM
                     KVFERHAPATAASQAGKITKLFSTLNKILSYRLSSDNLLALIRLTKRDAIYEPDTKRQ
                     RTDHIEEYRKRVVTQYERDRERLLRERHENAVSLDIRELFGGTEVLVLDGYNEEVDSY
                     LRRESPNGFTHVKPMGILKTFVKGVFETQIKETVKKVLVEGYFDNKNFQNSLANILFQ
                     CDRTGPRVEAFEEGLKSNARVSVAAVKRYVEEMRHGKDIMPFLTKLVDEINYKAREIC
                     EDETGLFQMLAETLGELLADYKKPSPDLVTNIRSLGGVRNKELLATLSEGKRKAETFV
                     RVMRNFAFVKTRDGLPPAPGALSAIRSDAPGRVEEAVEVEPLP"
     gene            complement(2434..2874)
                     /locus_tag="PLQ29_04110"
     CDS             complement(2434..2874)
                     /locus_tag="PLQ29_04110"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPG85861.1"
                     /translation="MSEELVRVRIRSVEMPSEDASPVVVLEEASGGGEFSVAVGAAEA
                     GAILLELEGIATPRPLTHELMVQILREQGLTIRRIEIYGLYGADEDGFLARLEYSKGF
                     RSWIRDVRPSDALALAVASGAPVFAHRSLLYGASDDWLEEARRA"
     gene            2975..3421
                     /locus_tag="PLQ29_04115"
     CDS             2975..3421
                     /locus_tag="PLQ29_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85862.1"
                     /translation="MKRSPLFLAFSGLELARYFALVHAVRVFCSAAPAAGPALRIAAA
                     PNALFAAAFLFLGLDTERYAGFKPLLVVGKALSLFGGLVALPGMLRLGAAGQSESVAA
                     YLLVGIAAWDALSALPLLVPAVDRGRSESVPPPMPPNAEPERVEPD"
     gene            3421..4566
                     /locus_tag="PLQ29_04120"
     CDS             3421..4566
                     /locus_tag="PLQ29_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HPG85863.1"
                     /translation="MRIIPVASGKGGVGKSLVSANLAIALAQAGRRVVLADLDLGASN
                     LHLILGFKAYTHGLGTYLSGAKIRFEDIIYDTDYPNLRFIPGDAEIPGLANIAYRQKT
                     QLAKRLLSVEDADYLILDLGAGTGANILDFFLLSGRGIVVTTPTLTATLNAYLFLKNA
                     VFRLMWDSFKPKSKARALLESLKKDGASLQRAYIPEILGRVSELDPASRAAFDMRMRK
                     LSPRLVLNMLEDPKDAEKAQKIRRSCREYLGMDLEHLGIVYRDDMQDVALGSRIPIIR
                     YKPQSILSQAIYRLADKLLSSEDDDDAFLDPEYLDDSYGAADTEAEADFDAKLDYVEE
                     LLSSGALTQGELIETIRMQQYELGQLRKENQFYKAKMAKALAQGFKA"
     gene            4611..>5573
                     /locus_tag="PLQ29_04125"
     CDS             4611..>5573
                     /locus_tag="PLQ29_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85864.1"
                     /translation="MLVDELGLRASFRFEPSADGPEYGVDGLARKLTEARIAGVQGRR
                     LDEIAKTFAVAKAPVTETVASGHAPEAGLPEAAEWAEPSTPDEYLPFAAEALSAAPPP
                     ELYRVRVERVARERIVKKTSALPFLPPKEEKVVDYEKIELREPIAVDTRVIMSFWAPK
                     GATVAALAPPRPGKPGKSVYGKPIPAPRVEDSAFYLGKGLSRVRNEVKAEVGGFVRVG
                     ERWADLIPFGSGDYSVRLSDDGATALLDYTPGDKRLPPPDAAAILEAAHALGLGAETL
                     IGAEETASALLRSTRSGQPLVGFSLSCDRDASARVDVSPDRLRAT"
BASE COUNT          910 a         1837 c         1819 g         1007 t
ORIGIN      
        1 tccgccctgc tccggggcga ggagcctcgc gcctcgtcgt ccggcgccga cccggcgctg
       61 tcgctgctac cgctgcatcc cgccgccgac cttatccgcg gcgctatccg ttccgaagcc
      121 cccgacgtgg tcgtcgaggc ggtattcctg tggcggaggc gcggcccggc gacggcggac
      181 gaggtcttgt tggtctataa cgccctcagg tcgatcggga gcctcgaggg tatccagtac
      241 tactcggcca gccgaggccg gacgcgcacg ctctacgagc gctccaccct gctggccggc
      301 ccgaactcgg atcgggcgct caccgacacc cggctccagg cactgccggc cggcggcgag
      361 acgctctatg cgctccagcg tgatacgacc ttcggcgaga accgttaccg cgtcgagctg
      421 tctgccggcg atggctatgt ggcgcagtcg tcgtcgaacc tgaccgggat gcggctagga
      481 ctcgtccccg tggcggggcc gggcgacgtg gtcgtacggg ttttggtcat ccaggtcgac
      541 gaggggctgc tattctacgt cgcctcgagc gccaaggccg ccgtggtacc gggggtccgg
      601 ggcagtctcg agcgatcctt cgcgaaccgg gcggccgcgg ccttcgcgtg gttctccggc
      661 atcatggacg ccgccgcgcc gagcggccgc tagggaagcg gttccacctc gacggcttct
      721 tcgacccggc ccggagcgtc ggaacgtatc gcgctgagcg ctccaggcgc cggcggcagg
      781 ccatcccgtg tcttgacgaa ggcgaagttg cgcatgaccc gcacgaaggt ctcggccttg
      841 cgcttgccct cggatagggt ggccaggagc tccttgttgc gtacgccccc gaggctcctg
      901 atgttggtga cgaggtcggg gctcggcttt ttatagtcgg ccagcagctc gccgagggtt
      961 tcggccagca tctggaacag gcccgtctcg tcctcgcaga tctcccgggc cttatagttg
     1021 atctcgtcga ccagcttggt caggaagggc atgatgtcct tgccgtggcg catctcttcg
     1081 acgtagcgct tgaccgccgc gacgctgact cgggcgttgc tcttgaggcc ctcctcgaag
     1141 gcctcgaccc gcgggccggt cctgtcgcac tggaacagga tgttggccag gctgttctgg
     1201 aaattcttat tatcgaaata gccctcgacg aggactttct tgaccgtctc cttgatctgc
     1261 gtctcgaaga cgcccttgac gaaggtcttg aggatgccca tcggtttgac gtgggtaaag
     1321 ccgttcgggc tctctcgccg caggtaggag tcgacctcct cgttatagcc gtcgaggacg
     1381 agcacctcgg tgccgccgaa cagctcgcga atatccaggg atacggcgtt ctcgtggcgc
     1441 tcgcggagca gcctttcccg gtcgcgctcg tactgcgtga cgacgcgctt gcggtattcc
     1501 tcgatatggt ccgtcctctg ccgcttggta tcgggctcgt agatggcgtc gcgcttggtc
     1561 agcctgatca gggcgagcag gttatcgctg gataggcggt acgagagtat cttgttcaag
     1621 gtcgagaata gcttggttat cttgccggcc tggctggcgg cggtcgccgg cgcgtgccgc
     1681 tcgaagacct tcatcgtctt ccggagcagg ttctccgaga aggagaagcc gatgagcagg
     1741 tagtaggcgt cgatgagttc gggcaggacc tgctctccgg cgcagggctg gaaatcgggt
     1801 cggtagcggg agtcgtcaag actggcgccg ggatcgaaga agccgagggt gcgctcccag
     1861 tcgtgcctgc agacctcgat gaagcgctcg acctcgtgca ggtcggtgtt gagaggaccg
     1921 tccgacagcg cttcgaggct tttgaggaaa tgctggaagt ccttggccac ggcttcctgt
     1981 tcctcgtcgt ccctgacggc gttctcgatg cgggccttca tgccgtcgta ggcgaagctc
     2041 tccttcttct caagctcctg gggcggcagc tcgaggtcga cgaggtagtc gaaatagcgt
     2101 ctggagacgc gcaggtccgg atgggccagg gtccggtcgg ccagatccat gagcggtttg
     2161 atcgcctggc agaacaggta cagatcctgc gcgaagccgg tcatgacgag attgttcttg
     2221 aagcggtaat acggcggctt gatcggcgag atgaccgagt agatgcgctt cagctcgcct
     2281 ttcgcgccta tctcgagacc gaggaggccg cggaagaacg ataggattgc ctgtaggatg
     2341 cccatggcca tctattctac ggcaggatgg gctcggcgac aagcgtgagc ggcccctggt
     2401 gttacgaacc ttattggtcc ccggcgatga aggctaagcc cggcgagctt cctccagcca
     2461 gtcgtcagac gcgccgtaga gcaaggagcg gtgggcgaag accggcgctc ccgaggcgac
     2521 cgccagggcg agcgcgtccg aaggccgcac gtcgcggatc cagctacgga atcccttcga
     2581 gtactcgagc ctggctagga agccgtcctc gtcggctccg tacagtccgt agatctcgat
     2641 ccgacgtatc gtcaggcctt gctccctgag gatctggacc atcagctcgt gggtaagcgg
     2701 tcgcggcgtg gcgatgccct cgagctcaag cagtatcgcg cccgcctcgg ccgcgccgac
     2761 cgcgaccgag aactcgccgc cgccagaggc ttcctcgagc actacgacgg gggaggcgtc
     2821 ctccgatggc atctcgacgc tccgaatcct gacgcgcacc aattcttcgc tcataccgga
     2881 aagtatagta cggcttccgg gtttttcacg cccgggccgg gacgcgagct tccttgacta
     2941 ctcggcgagc agccgagtac acttccattc tattatgaag cggtcaccac tcttccttgc
     3001 gttctcgggc ttggaactcg ccaggtattt cgcgctcgtc cacgccgtcc gggtattctg
     3061 ctcggccgcc ccagcggcag gccccgcgct caggatagcc gcggctccga acgcgctgtt
     3121 cgcggctgcc ttcctgttcc tagggctcga tacggagcga tacgccggct tcaagccgct
     3181 cctggtcgta ggtaaggccc tgagcctgtt cggcggcctc gtggcgttac ccgggatgct
     3241 cagactcggc gccgcgggtc agtccgagtc ggtcgccgcc tacctcctcg tcgggatagc
     3301 cgcgtgggac gcgctgtccg ccttgccgct cctggtcccg gccgtggacc gaggccgaag
     3361 cgaatcggtt ccaccgccca tgccgccgaa cgccgagcca gagcgcgtgg agccggactg
     3421 atgcgtatca tacccgtagc gagcggcaag ggcggcgtcg gcaagtccct cgtctccgcc
     3481 aacctggcta tcgccctggc ccaggccggc cgcagggtcg tactggccga tctcgacctc
     3541 ggcgcgtcga acctgcacct gatactcggc ttcaaggcct atacgcatgg cctgggcacc
     3601 tacctgtcgg gcgccaagat acgcttcgag gatatcatct acgataccga ctacccgaac
     3661 ctgcgcttca tccccggcga cgcggagatc cccgggctgg ccaatatcgc gtaccggcag
     3721 aagacgcagc tggccaagcg gctcctgtcc gtcgaggacg ccgactacct gatactcgat
     3781 ctcggcgccg gcaccggggc caatatcctc gacttcttcc tgctgtccgg ccgcggcatc
     3841 gtcgtgacga ccccgacgtt gacggccacc ctcaacgcgt acctgttcct gaagaacgcg
     3901 gtgttcaggc tcatgtggga cagcttcaag cccaagtcca aggcccgcgc cctgctcgag
     3961 tcgctcaaga aggacggcgc ctcgctccag agggcctata tccccgagat actcgggcgc
     4021 gtctccgagc tcgatcccgc gagccgcgcc gccttcgaca tgcgtatgag gaagctgtcg
     4081 cccaggctcg tgctcaacat gctcgaggat ccgaaggacg ccgagaaggc ccagaagatc
     4141 cgccgatcct gccgcgaata cctcggcatg gatctcgagc acctcggcat cgtctaccgc
     4201 gacgacatgc aggacgtggc gctcggtagc cgcataccga tcatccgcta taagccgcag
     4261 tccatcctgt cgcaggctat ctaccgcctg gcagacaagt tgctctcgtc cgaggacgac
     4321 gacgacgctt tcctcgaccc cgagtacctc gacgacagct acggcgccgc cgatacggag
     4381 gccgaggcgg acttcgacgc caagctcgac tatgtcgagg agctcctgtc gtccggcgcg
     4441 ctgacccagg gcgagctgat cgagacgata cgtatgcagc aatacgagct cggccagtta
     4501 cgtaaggaga atcagttcta caaggccaag atggcgaagg ccctagctca gggcttcaag
     4561 gcctaggagc cgaagcgatg gccggagcga cctttcgagg ggagacgacc ttgctcgtgg
     4621 acgagctcgg gctgcgggct tcgttccgtt tcgagccctc ggccgacggc ccggagtacg
     4681 gcgtcgatgg gctggccagg aagctgactg aggctaggat cgccggcgtc cagggccgca
     4741 ggctcgacga gatcgctaaa accttcgcgg ttgccaaggc gcccgtgacc gagaccgtag
     4801 cctccggaca cgcgcccgag gccggattgc ccgaggccgc cgagtgggcg gagccgagca
     4861 cgcccgatga gtacctgcct ttcgcggcgg aggccctctc cgccgcgccg cccccggagc
     4921 tgtaccgcgt cagggtcgag cgcgtagcca gggagcggat cgtcaaaaaa acgtcggcgc
     4981 tacctttcct gccgccgaag gaggagaagg tcgtcgacta cgagaagatc gagctgcgcg
     5041 agccgatagc tgtcgacacg agggtcatca tgagcttctg ggcgcctaaa ggagcgacgg
     5101 tggccgccct cgcgccgccc cgcccgggca agccgggcaa gagcgtctac ggcaagccga
     5161 tcccggcgcc acgggtcgag gactcggcct tctacctcgg caagggcctt tcccgagtta
     5221 ggaacgaagt gaaagccgaa gttggcggtt tcgtccgggt cggcgagcgc tgggccgacc
     5281 ttatcccctt tggctccggg gactactcgg tgcggctgtc ggacgacgga gccaccgccc
     5341 tgctcgacta tacccccggc gacaagcgcc tgccgcctcc cgacgccgcc gcgatactcg
     5401 aggctgccca cgccctcggc ctgggcgccg aaaccctgat aggcgccgag gagacggcct
     5461 cggcgctgct caggtcgacg aggagcggcc agcccctcgt cggcttcagc ttgtcctgtg
     5521 accgcgacgc ctcggcgagg gtcgacgttt cgcccgacag gctcagggcc acg
//
LOCUS       DAOPHV010000106         5564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_19285_length_5564_cov_4.069704, whole genome shotgun sequence.
ACCESSION   DAOPHV010000106 DAOPHV010000000
VERSION     DAOPHV010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19285_length_5564_cov_4.069704"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PLQ29_04130"
     CDS             <1..541
                     /locus_tag="PLQ29_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPG85865.1"
                     /translation="GRVRITKIVGDIEKNIDILSAPEIFGEMAILEGAPRSATAIAVE
                     TVKVLEFNRANFEILMMGNPQIALRLLKVFTKRIYDQKRRFMILTLDDPQAKIADVFL
                     MLDETQGALNQGEELGDKRDFRVTVDDVAHWAGMNPAEVQTLLSHFSNQRRIELYSDH
                     IVVKNINDFIRFVNSKRKK"
     gene            617..689
                     /locus_tag="PLQ29_04135"
     tRNA            617..689
                     /locus_tag="PLQ29_04135"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:650..652,aa:Thr,seq:cgt)
     gene            850..2085
                     /locus_tag="PLQ29_04140"
     CDS             850..2085
                     /locus_tag="PLQ29_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPG85866.1"
                     /translation="MKRARLILSVAMALVLVAGAAAQTITVGSWRTDDVAQWKGLIAD
                     FNKSYPGIEVRFDPTNPPDYNATLRLQLESGTGPDVFYARSGDVGKTLFKDGFMFDLS
                     AEPFMSKFDAASKFFWQSDDGRSFGMPLFAISQGIYYNVKLFRDYKIAIPQTWEELLA
                     AAKTLKSKGVIPFANGTKDEWDINEVLFQTLAPTYVGGAAGRKALESGAKKWNDASVA
                     AVFSAVKDLSPYLPRGYAAVDYNSSKVMFQLGKAAMMFDGSWTIPEHMKAAGLEYGVF
                     AAPAPKGMKRAQVFHADTGIAINPKSKNVAAAKTFVAWLVSDAGIASVAENFTGFFPM
                     AKASKAIPFSNPVVKSFLAMNDGVETDVRFSWTRLNTGNPSGYDLITANTIAVLGGKK
                     TPKQAADDFAADLAKWYVY"
     gene            2183..3121
                     /locus_tag="PLQ29_04145"
     CDS             2183..3121
                     /locus_tag="PLQ29_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPG85867.1"
                     /translation="MARSGRRSPWGVGIYRRPSWFLYILPALAVYVAFMAFPLLSSLK
                     LSFYSGSGFTLDTFVGFDNYVRLFADADVAPRFWGAFGNTWVFFFVHMLVQNTLGLMF
                     ALILSDKRLRGAGFFRTVIFIPTTLAIVVTGFLWKLMLDPNFGPINLLLDRVGLGALA
                     LPWLGLEETTLPIISLVSSWQWVGIPTMIFTAALQSIPEDYFEAAQIDGASGWQVTRL
                     IKLPIIVPMMGVVGVLTFTGNFNAFDVVYSMAGANGAPNYAADILGTYFYRTGIAGKH
                     PVGIPDMGLGAAIATVTFVTLLAGVMVIRRLTMGRK"
     gene            3132..3989
                     /locus_tag="PLQ29_04150"
     CDS             3132..3989
                     /locus_tag="PLQ29_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPG85868.1"
                     /translation="MPRVNDGGSARRRLRASRMGSYLVLCAWAVVVLVPLYLMIVNSF
                     KSTSDIFMSPFSFPKNPVFDGFRMVFEEGHFLLYLFNSVLITVLAIVGVMLLAALASY
                     ALVYWDVRLARAVSLFFLLGLMIPMRIASINLIIMLKGMGLLDKLLGLLPIYLAMGMP
                     MGVMILTEYFRTIPRELVQAAYIDGAGSFRIFRSVMLPLARPAMATVAIFNLVTIWND
                     LWFPLILIRSEGQRTLMLGVTRLFGQFQTDWTSVLATLTLATAPILALYLLLSRQFIE
                     GLTAGAIKE"
     gene            complement(4002..4655)
                     /locus_tag="PLQ29_04155"
     CDS             complement(4002..4655)
                     /locus_tag="PLQ29_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPG85869.1"
                     /translation="MTRILIVDDQRLFAESLRTVLEGTQDDFRVCALAGDGAQAVAAV
                     AEHQPDIVLMDVSMPAMDGVEAARIIHNRYPELPILMLTTFEDDGYVREAIAYGARGY
                     LLKNIPSRELVASIRAVLSGAMLLDPRVVGALVGKGRDGPASGARAASPEWFDSLSRR
                     ERQILGLIVDGLQNDEIAQRLCVAPQTVRNYVSRLYDKTATGDRLRLIRLARASGLF"
     gene            complement(4652..>5564)
                     /locus_tag="PLQ29_04160"
     CDS             complement(4652..>5564)
                     /locus_tag="PLQ29_04160"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPG85870.1"
                     /translation="LPRPWDLAGAAIAIASALGTQAGRKVWETIVEPASIDELAFLAA
                     LPALVGLVAGGYKDALLGFVRSAREVERLRDGSEAIIMANVRFQEQARELEDESSDRE
                     RKRISREIHDIVGYAITNQAMAMEAALVLLDKGDREDELRTLLEESRDQSREGLAEVR
                     AALYRLREQRAPYPDFQNRMIHICRTFGKATGVEVSCEVLGEDARIPAGLDAALYRIV
                     QAGLTNSFLHGKARKVRVLIQSQDGTLHLSIQDDGVGAEQVYEGIGLAGMRERLAPYG
                     GTLAYENTGYGFLVRASAPLDGTREGS"
BASE COUNT          932 a         1849 c         1762 g         1021 t
ORIGIN      
        1 cgggcgggta cgcatcacca agatcgtcgg cgacatcgag aagaacatag acatactctc
       61 ggctccggag atattcggag agatggccat actcgagggc gcgccccgtt cggctaccgc
      121 catagccgtc gagacggtca aagtgctcga gttcaaccgg gccaacttcg agatcctgat
      181 gatgggcaac ccccagatcg cgctccggct actcaaggtg ttcacgaagc gcatctacga
      241 ccagaagcgt cggttcatga tcctgacgct cgacgatccg caggccaaga tagccgacgt
      301 attcctgatg ctcgacgaga cccagggggc gctgaaccag ggcgaggagc tcggcgacaa
      361 gcgcgacttc agggtgacgg tcgacgatgt cgcccactgg gccggcatga accccgccga
      421 ggtgcagact ctgctcagtc acttttcgaa ccagcgccgc atcgaattgt actcggacca
      481 tatcgtcgtc aagaacatca acgacttcat ccgcttcgtc aactcgaagc gcaagaagta
      541 ggcggcgctc cctttcccgg cgttcccttt ccccttgcat cgcagggccc gtttcaggta
      601 tagtctcgcg cgtatcgccg agatagctca cttggcagag cggcgcactc gtaatgcgca
      661 ggtaccgagt tcgattctcg gtctcggctt gaggccgcgc cttccgtacg gaaggcgcgg
      721 ccttttcttt ccggagcggc cgtcgacccc ggtacaaatc gagtacaatc cgagtactcg
      781 gcaaccatat cgcgatatac gatacgcccc tacacttcgg atagcgaaac ctaagtaaag
      841 gagctaccga tgaaacgagc aaggcttatc ctgtccgtcg ccatggcgct ggtcctggtc
      901 gccggagccg cggcgcagac catcacggtc ggatcgtgga ggaccgacga cgtcgcccag
      961 tggaaggggc tcatcgccga cttcaataag tcgtaccctg gcatcgaagt caggttcgac
     1021 ccgaccaacc cgcccgacta caacgctacg ctacggctcc agctcgagag cggcaccggc
     1081 ccggacgtgt tctacgcccg ctccggcgac gtgggcaaga ccctgttcaa ggacggcttc
     1141 atgttcgatc tgagcgccga gcccttcatg tccaaattcg acgccgcctc caagttcttc
     1201 tggcagtccg acgacggccg ctccttcggc atgccgctgt tcgcgatcag ccagggcatc
     1261 tactacaacg tcaagctatt ccgcgactac aagatagcca tcccccagac ctgggaggag
     1321 ctgctcgcag cggccaagac gctcaagtcc aagggcgtca tccccttcgc caatggcacg
     1381 aaggacgagt gggacatcaa cgaggtgctg ttccagaccc tggccccgac ctacgtcggc
     1441 ggagcggccg gccgcaaggc tctcgagtcg ggcgccaaga agtggaacga cgcgtcggtc
     1501 gcggccgtct tctccgcggt caaggacctg tccccctacc tgccgcgcgg ctacgcggcg
     1561 gtcgactata actcgtccaa ggtgatgttc cagctgggca aggccgcgat gatgttcgac
     1621 ggctcctgga ccattcccga gcacatgaag gccgccggcc tcgagtacgg cgtcttcgcc
     1681 gccccggcgc ccaagggcat gaaacgggcc caggtcttcc acgccgacac cggcatcgcg
     1741 atcaacccga agtccaagaa cgtggccgcg gccaagacct tcgtcgcctg gcttgtcagc
     1801 gacgcgggta tcgcgtcggt ggccgagaac ttcaccggct tcttcccgat ggccaaggcc
     1861 tcgaaggcga tcccgttctc gaacccggtc gtcaagtcct tcctggccat gaacgatggc
     1921 gtcgagaccg acgtacggtt cagctggacc cgcctgaaca ccggcaatcc ctccggctac
     1981 gacctgatca ccgcgaacac catagcggtg ctcggcggca agaagacccc gaagcaggcc
     2041 gcggacgatt tcgcggccga cctggccaag tggtacgtct actgatccgg cgcacctggc
     2101 tctagtgttc aagtagcccc ggtcgcagcc ggccggggcg tattccttac tgaggatgga
     2161 cccgatgacg cagactcccg gtatggccag atccggccgc cgatcgccct ggggcgtggg
     2221 gatctaccgt aggccgagct ggttcctcta tatcctgccg gcgctggccg tctacgtggc
     2281 cttcatggcc ttcccgctcc tgtcgtcgct caagctgagc ttctacagcg gtagcggctt
     2341 cacgctcgat accttcgtcg gcttcgataa ctacgtcaga ctgttcgccg acgccgacgt
     2401 ggctccccgg ttctggggcg ctttcggcaa tacctgggta ttcttcttcg tgcatatgct
     2461 cgtacagaac acgctcgggc tcatgttcgc gttgattctg agcgacaagc ggctcagggg
     2521 cgctggcttc ttcaggactg tcatcttcat tccgacgacc ctcgccatcg tcgtcaccgg
     2581 cttcctctgg aagctgatgc tcgatcccaa cttcggcccg atcaacctcc tgctcgatag
     2641 ggtgggcctc ggcgccctgg ccctgccgtg gctcgggctc gaggagacga ccctgccgat
     2701 catctcgctc gtgtcgagct ggcaatgggt cggcatcccg acgatgatct ttaccgccgc
     2761 gctccagagc atcccggagg actatttcga ggcggcccag atcgacggcg cctccggctg
     2821 gcaggtgacg aggctcatca agctgccgat catcgtaccg atgatgggcg tggtcggcgt
     2881 gcttaccttt acggggaact tcaacgcgtt cgacgtggtc tactcgatgg ccggtgcgaa
     2941 cggggcgccg aactacgcgg ccgacatact cggcacgtac ttctaccgga ccggtatcgc
     3001 cgggaagcat cccgtcggta tccctgacat gggcctcggg gccgcgatcg cgacggtcac
     3061 cttcgtcacg ctgctcgccg gcgtgatggt catacggcgc ctgaccatgg gcaggaagta
     3121 agggggcgac gatgccgaga gtgaacgatg gcgggagcgc ccggaggcgg ctccgcgcct
     3181 cgaggatggg ctcctacctg gtactctgcg cgtgggcggt cgtcgtgctc gtgccgctct
     3241 acctgatgat agtcaactcg ttcaagtcga cttcggacat cttcatgtct cccttcagct
     3301 tcccgaagaa cccggtcttc gacggcttcc gcatggtctt cgaggagggg catttcctcc
     3361 tgtacctgtt caactcggtg ctcatcacgg tgctggccat cgtcggcgtg atgctcctcg
     3421 cggcgctcgc gagctacgcg ctggtatact gggacgtgcg gctcgccagg gccgtatcgc
     3481 tgttcttcct gctcgggctg atgatcccga tgcgcatagc gagcatcaac ctgatcatca
     3541 tgctcaaggg gatgggcctg ctcgacaagc tgctcgggct cctgccgatc tacctggcga
     3601 tgggcatgcc catgggcgtg atgatactga ccgagtactt ccggaccata cccagggagc
     3661 tcgtccaggc cgcctacatc gacggcgccg gctcgttccg catcttcagg agcgtgatgc
     3721 tgccgctcgc tcgcccggcg atggcgacgg tggcgatctt caacctggtg acgatctgga
     3781 acgacctgtg gttccccctc atcctgatac ggagcgaggg ccagcgcacc ctgatgctcg
     3841 gcgtgaccag gctgttcggg cagttccaga ccgactggac gagcgtgctc gcgacgctga
     3901 ctctggcgac ggcgccgatc ttggcgctct acctgctcct gtccaggcag ttcatcgagg
     3961 gcctgacagc cggcgcgatc aaggaatagg cgggacgcta gctagaacag gccgctggcg
     4021 cgggccagcc gtatgagcct gaggcggtcc ccggtggccg tcttgtcgta gaggcggctg
     4081 acgtagttcc ggacggtctg cggcgcgacg cagagccgct gggctatctc gtcgttctgg
     4141 aggccgtcca ctatgaggcc gagtatctgg cgctcgcggc gcgacagcga gtcgaaccac
     4201 tccgggctag cggcgcgagc ccccgacgcc ggtccatcgc gccctttccc gacgagggcg
     4261 ccgacgacgc gcgggtcgag cagcatggcg ccgctcagca cggcccggat gctcgccacg
     4321 agctcacggc tcgggatgtt cttaagcagg tagccgcggg cgccgtaggc tatcgcctcg
     4381 cgcacgtagc cgtcgtcctc gaaggtggtc agcatcagga tgggaagctc ggggtagcgg
     4441 ttgtggatga tcctggccgc ctcgacgcca tccatcgccg gcatgctgac gtccataagc
     4501 actatgtccg gctggtgctc ggcgacggcg gccacggctt gagcgccgtc gcccgccaac
     4561 gcgcagaccc tgaaatcatc ctgcgtgccc tcgagcacgg tcctgaggct ctcggcgaac
     4621 agccgctggt cgtccacgat caggatcctg gtcatgagcc ctcccgggta ccgtcgagcg
     4681 gcgccgaggc cctgaccagg aagccgtagc cggtgttctc gtaggcgagc gtcccgccat
     4741 acggggccag ccgttcgcgc atgccggcca gccctatgcc ttcgtagacc tgctccgcgc
     4801 cgaccccgtc gtcctggatc gacaggtgga gcgttccgtc ctggctctgg atgagtacgc
     4861 gtaccttcct ggccttgccg tgcaggaacg agttggtcag cccggcctgg acgatgcggt
     4921 agagcgccgc gtcgaggccc gccgggatcc gcgcgtcctc gccgagcacc tcgcaggaga
     4981 cctcgacgcc ggtagccttg ccgaaggtgc ggcagatatg gatcatgcgg ttctggaagt
     5041 cggggtaggg ggcgcgctgc tcgcggagcc tgtacagcgc ggcgcgaacc tcggcgaggc
     5101 cctcgcgcga ctggtcccgc gactcctcga gcagggtccg gagctcatcc tcgcggtcgc
     5161 ccttgtcgag cagcacgagg gccgcttcca tcgccatggc ctggttggtg atcgcgtagc
     5221 cgacgatgtc gtggatctcg cggctgatgc gtttgcgctc gcggtcggag ctctcgtcct
     5281 cgagctccct ggcctgctcc tggaagcgca cgttggccat gatgatggcc tcgctaccgt
     5341 cgcgcaggcg ctcgacctcg cgggcgctgc ggacgaagcc gagcagggcg tccttgtagc
     5401 cgccggcgac cagcccgacg agggccggca gggcggcgag gaaggccagc tcgtcgatcg
     5461 acgcgggctc gacgatggtc tcccagacct tgcggccggc ctgcgtgccg agggcggagg
     5521 ctatggcgat ggccgcgccg gccaggtccc agggccgcgg cagg
//
LOCUS       DAOPHV010000107         5545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_19372_length_5545_cov_4.232240, whole genome shotgun sequence.
ACCESSION   DAOPHV010000107 DAOPHV010000000
VERSION     DAOPHV010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19372_length_5545_cov_4.232240"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLQ29_04165"
     CDS             <1..412
                     /locus_tag="PLQ29_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85871.1"
                     /translation="RPSRPRNVCDHDLDIMGNPTTGSMLVEYREDATVLWFTGAPYAC
                     ANLFKPVLLSNGEFVPLWTDYDYVEGSGGGEAYWKARRAATRPLWKRPKGGHDTALVE
                     AQAALSAMVDETASPGAGSAVVNDIVRDWDARPL"
     gene            complement(414..1274)
                     /locus_tag="PLQ29_04170"
     CDS             complement(414..1274)
                     /locus_tag="PLQ29_04170"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPG85872.1"
                     /translation="MKARVRHFALRVAISYAAIAFLWIAFSDRLATLIAEDYAHLASF
                     QTAKGFFFVAATTALLYFFSKRQFETIVSVSTQRELDSQARLREKETLLREINHRVKN
                     NLQVILSLMRLTPKDERGFTDLDRKIRSIALAQDLLTSSPDMSSIRARDFAQSLASTF
                     AGGFIPSELRLSGSGDDTALSADAAVAFGILVAEACSNAARYGRSGSAPVSVCVSIRS
                     VDGSVAATVRDDGPGFPLEVLSRQLAADTSLGISLMEALAVQVSGELSLRNEPGATVE
                     LRIPGPGLAD"
     gene            complement(1325..2506)
                     /locus_tag="PLQ29_04175"
     CDS             complement(1325..2506)
                     /locus_tag="PLQ29_04175"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HPG85873.1"
                     /translation="MSDAESLLHLGRSEAIDLAYRARFSFDVPPDAIESAAAQLRSLG
                     HVQIDTISVVERAHHHVFWSGDDAYGPECLPALERPPRRALEYWAHAAAYLPIEDYRY
                     CLPRMRRIAETGHQWFPTDPEVVRAVLDRIRAEGPLRSCDFAGGDGPRGPWWDWKPAK
                     AALEFLFMSGVILVAGRPGFQKLYDLAERVLPPDIDDRTPSDDEMADWYVRRAACAYG
                     VFCAADAAYQRKEATAGVGAALERAAESGSIVEARLEGDEARRYWVSTKALECPRRPD
                     DRPVRLLSPFDNYIIDRKRARRLLGLEYTLECYVPEAKRAFGYFALPIVWRGRPAGLV
                     DAAADRKRKALVIKRVDLRPRPEELPGASIDEFRSMLIRELRAFAAFNGCDTIVGEPA
                     I"
     gene            complement(2503..3063)
                     /locus_tag="PLQ29_04180"
     CDS             complement(2503..3063)
                     /locus_tag="PLQ29_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HPG85874.1"
                     /translation="MKTIRSYLYGRVEVAVGDITLYDGDAIVNAANSALAGGGGVDGA
                     IHRAAGPELSAECRALRMGSLPDGLPTGRAVATGAGRLAVRGVIHAVGPIWRGGTADE
                     SRLLASAYTEALSIAEARGWDRVAFPAISTGVYGYPKALAARVAFEAVSSYLAARDRP
                     RLVSLVFFSQADAEAFMASIGEPEAS"
     gene            3120..3962
                     /locus_tag="PLQ29_04185"
     CDS             3120..3962
                     /locus_tag="PLQ29_04185"
                     /inference="COORDINATES: protein motif:HMM:NF015546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85875.1"
                     /translation="MLQEDGSYPLIEPFDDFHDCADRLAAAGADEFAFFPFIDSDAVE
                     RAALGRFMPMGLSVELEGRRCDLLLPKLHLERCLLDPCDTRVTRTARRESRRYSLSVN
                     RAFGAVLSSCVATHGADWLVGDLERTFLELHEQRFQRRVAFVSVELWVGSGPDLVAGE
                     IGYVVGGAYASLTGYTARSGAGTVQLMALGGLLAKLGLRVWDLGMAMDYKLALGGRAL
                     PRESFMRELDRAYRDDDAPGNAAIRAVSGLVPARAFLDASASLPEGFLADSTNVPDTG
                     DHHE"
     assembly_gap    3993..4092
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4093..4491
                     /locus_tag="PLQ29_04190"
     CDS             <4093..4491
                     /locus_tag="PLQ29_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85876.1"
                     /translation="VAKAIGTLRLGVYFSLGAGPTGLFALLTGVGPGPSLAWSAGAGL
                     FIATLSKALRAFIRKDLDSSIKPAEFIMDEAIVTVPVLPGEMGKATVRSYGKETDLYV
                     RSKDARATFPKGSAVRIVDFDDDCFWIEPV"
     gene            4540..>5545
                     /locus_tag="PLQ29_04195"
     CDS             4540..>5545
                     /locus_tag="PLQ29_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPG85877.1"
                     /translation="MLSEFFSGFQGVLFGALGVVAFLALVGLLKATIRVASPDRILVV
                     TGRKTRRHGREFGFTVDRGRTVVIPYFQAVGTLDLGVFPINVRVEGVNSANGITMGAD
                     ATACVCIDDDDEAMLYSAVERLMGKDVRQIHEQIQQTLVGNFRGALNKATPLQAIGME
                     DVNSDDGNVSADLGERAQFRAELLADINSDLRSFGMKVVSVSLQKIWDTSNYIANLAQ
                     KTLAEKRQAVEIEESRLRAIADQAESEARKRIEVARSQADEAIISARQELEVYRRESS
                     GLIEEAKLKADQAIAEARNSGESAVQAQLVELQKLRNQSSVTLVAAAREEAARILAEG
                     E"
BASE COUNT          925 a         1736 c         1844 g          940 t
ORIGIN      
        1 tcgcccctcc aggccccgca acgtttgcga ccatgacttg gatatcatgg gcaacccgac
       61 gaccggctcg atgctggtgg agtatcgcga agacgcgacc gtactgtggt tcacgggcgc
      121 tccgtatgcc tgcgccaacc tgttcaagcc tgtgctcctg tccaacggcg aattcgttcc
      181 actctggacc gattacgact atgtcgaggg ctcgggcggc ggggaggcct actggaaagc
      241 gaggcgggcc gcgacgcgac ctctgtggaa acggccgaag ggtggccatg acaccgccct
      301 cgtcgaggcg caggcggctc tgtcagccat ggtcgacgag accgcgtccc cgggggccgg
      361 ctcggcggtc gtgaacgata tcgtacggga ctgggacgct cgcccgctct gagtcagtcc
      421 gctaagccgg gccctgggat acgtagctcc accgtggcgc caggctcgtt gcgtagcgac
      481 agctcgcccg agacctggac ggccagggcc tccatcagcg atatgccgag cgacgtatcg
      541 gcggcgagct gcctcgagag cacctcgagg gggaatcccg gcccgtcgtc gcgcacggtc
      601 gccgcgacag agccgtcgac cgaacgtatc gacacgcata ccgagacggg agcggacccg
      661 ctacggccgt atctcgccgc gttcgagcag gcctcggcta ccaggatgcc gaacgccacg
      721 gcggcatccg ccgatagcgc ggtgtcgtcg cccgagccgc tcaggcgaag ctcggagggg
      781 atgaagccgc cggcgaaggt cgacgcgagg ctctgggcga aatcccgggc cctgatcgaa
      841 gacatgtcgg gcgacgaggt caggaggtcc tgagccaggg cgatggagcg gatcttgcgg
      901 tcaaggtcgg tgaagccccg ttcgtccttg ggcgtgagcc tcatcaggct gagtatcacc
      961 tgtaggttgt tcttgactcg gtggttaatc tcgcgaagca gggtttcctt ctcgcgcaag
     1021 cgggcctgcg agtcgagctc ccgctgtgtc gaaaccgata cgatcgtctc gaactggcgc
     1081 ttcgagaaaa aatagaggag agctgtcgtc gcggcgacga agaaaaagcc cttggcggtc
     1141 tggaagctgg ccaggtgggc gtagtcctcg gcgatcagcg tcgccagcct gtccgaaaag
     1201 gctatccaga ggaaggctat ggccgcgtaa ctaatagcga ctcgtagggc gaaatgccgt
     1261 actcgagctt tcatctatcc ctccacgccc tccgttatat cgataagcgc tcggcccgct
     1321 tttgtcaaat cgccggctcc ccgacgatgg tatcgcagcc gttgaaggcg gcgaaggctc
     1381 gcagctcccg gatgagcatc gagcggaatt cgtcgatcga cgcgcctgga agctcctcgg
     1441 ggcgcgggcg cagatcgacg cgcttgatga cgagggcttt gcgcttccta tcagccgcgg
     1501 cgtcgacgag gcccgccgga cgtcccctcc agacgatcgg cagggcgaag tagccgaatg
     1561 cgcgcttggc ctcggggacg tagcattcga gcgtgtattc gaggccgagt agcctgcgcg
     1621 cccgtttccg gtcgatgatg tagttgtcga acggggatag caggcggacc ggcctgtcgt
     1681 ccggtcgccg cggacattcc agggctttcg tcgagaccca gtagcgcctg gcttcatcgc
     1741 cctcgagcct ggcctcgacg atggagcctg actccgcggc tctctccagg gccgcgccga
     1801 cgccggccgt ggcttccttc cgctggtagg ccgcgtcggc cgcgcagaag acgccgtagg
     1861 cgcatgcggc gcggcgtacg taccaatcag ccatctcgtc gtcgctcggc gtcctgtcgt
     1921 ctatatccgg cggaaggacg cgctcggcca ggtcgtacag cttctggaaa cccgggcggc
     1981 cggccacgag tatgacgcct gacatgaaca ggaactcgag cgcggccttg gctggcttcc
     2041 aatcccacca gggacctcgg ggaccgtctc cgccggcgaa gtcgcaggag cgtagcggtc
     2101 cctcggcccg tatcctatcg agcacggccc gtactacctc gggatccgtc gggaaccact
     2161 ggtgcccggt ttcggctata cgccgcattc gcgggaggca gtaacgatag tcttcgatgg
     2221 gcaggtaggc ggccgcgtgg gcccagtact cgagcgcccg cctgggcggc cgctccagag
     2281 ccggtaggca ctcgggcccg tacgcgtcgt ctccgctcca gaacacgtgg tggtgggccc
     2341 gttcgacgac cgagatggta tctatctgga cgtggccgag cgatcgcagc tgggccgccg
     2401 cggattcgat cgcgtcgggc ggtacatcga aactgaatct ggcccggtac gcgaggtcta
     2461 tcgcttcgga acggccaagg tggaggaggc tctcggcatc gctcatgagg cttccggctc
     2521 cccgatcgac gccatgaagg cctcggcgtc ggcttgagag aagaagacca agctgacgag
     2581 tcgcggcctg tcgcgggcgg ccaggtacga cgatacggcc tcgaaggcca ccctcgcagc
     2641 gagcgccttc gggtagccgt agacgcctgt ggagatggcc gggaaggcga cgcggtccca
     2701 gccccgtgcc tcggcgatcg acagggcctc ggtataggcc gacgccagca ggcgcgactc
     2761 gtcggcggtt ccgcctcgcc agatcggccc gacggcgtgg atcacgccgc ggacggccag
     2821 ccggcccgct ccggtcgcta cggcgcgccc cgtaggcagt ccgtcgggca aggatcccat
     2881 gcggagggcg cggcattctg ccgacagctc tggcccggcg gctcggtgga tggccccgtc
     2941 gacgccgccg cccccggcca gcgccgagtt ggccgcgttg actatggcgt cgccgtcgta
     3001 gagcgtgata tcaccgaccg cgacctcgac gcgtccgtag aggtacgagc gtatggtttt
     3061 catggccgat agtatatcgt atatactggt cgtatccgat cgcgcctcga gggatttaga
     3121 tgctccagga agacggctcc tatccgctca tagaaccctt tgacgacttt cacgactgtg
     3181 ccgaccggct ggccgcggct ggagccgacg agttcgcctt cttcccgttc atcgattcgg
     3241 acgccgtcga acgggcggcg ctcggccgct tcatgcctat ggggctgagc gtcgagctgg
     3301 aaggtcggcg atgcgacttg ctcctgccca agctgcacct cgaacgctgc ctgctcgacc
     3361 cctgcgatac gcgcgtgacc aggaccgcca ggcgcgagtc gcggcgatac tcgctgagcg
     3421 tcaaccgcgc cttcggggcg gtgctgtcgt cgtgcgtggc gactcacggc gccgactggc
     3481 tcgtcggcga cctggagcgg accttcctgg aactgcacga gcagcgtttt cagcggcgcg
     3541 tggccttcgt ctccgtcgag ctatgggtcg gtagtgggcc ggacctggtc gccggcgaaa
     3601 taggctatgt ggttggcggc gcctacgcca gcctgaccgg atacacggcc cgctctggcg
     3661 caggcacggt gcagctcatg gcgctcggag gcctgctcgc caagctgggc ttacgggtct
     3721 gggatctggg catggcgatg gactacaagc tggctctcgg cggcagagcc ctgcctcgcg
     3781 agagcttcat gcgggaactg gacagggcgt atcgggacga cgacgccccg ggcaacgcgg
     3841 cgatacgggc cgtatccggc ctggttccgg ccagggcctt tctggacgcc tcggcgtcgc
     3901 taccggaagg ctttcttgcc gattcgacga atgtacctga tactggagac catcatgaat
     3961 agcttgttcg ttctatacac ggccctggcc gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nngtcgccaa ggctatcgga accctgcgcc tgggcgtcta tttctcgctc
     4141 ggcgccggac cgaccggcct gttcgcgttg ctgacaggcg tcggacccgg tcccagcctg
     4201 gcttggagcg ctggcgccgg cctgttcatc gcgacgctct cgaaggcgct acgggccttc
     4261 atacgcaagg atctggattc gtccatcaag cccgccgagt tcatcatgga cgaagccatc
     4321 gtcacggtgc cggtgctgcc cggcgagatg ggaaaggcga cggtccgcag ttatggcaag
     4381 gagaccgatc tgtacgttcg gtcgaaggac gcccgcgcga ccttcccgaa gggcagcgcc
     4441 gtgcgtatcg tcgatttcga cgacgactgc ttctggatcg agccggtgtg atactatacg
     4501 tgcatatgaa gatggctgca tgaaaaagga ggggttggga tgctgagcga attcttctcc
     4561 gggttccaag gcgtgttgtt cggggccctc ggggtcgtcg ctttcttagc cctggtcggg
     4621 ctgctcaagg cgaccatacg cgtcgcgtcg ccggaccgca tactcgtcgt gaccggccgt
     4681 aagactaggc ggcacggccg cgagttcggt ttcacggtag accgcggcag gacggtggtc
     4741 ataccgtatt tccaggctgt cggtacgctc gacctcggcg tgttcccgat caacgtgcgg
     4801 gtcgaaggcg tgaacagcgc caatggcatc acgatgggcg ccgacgccac ggcctgcgtc
     4861 tgcatcgacg atgacgacga ggcgatgctc tacagcgccg tcgagcgcct gatgggcaag
     4921 gacgtgcgtc agatccacga gcagatccag cagaccctcg tcggcaactt ccggggcgcg
     4981 ctcaataagg ccacgccgct ccaggccatc ggtatggagg acgtcaactc cgacgacggt
     5041 aacgtatcgg ccgacctcgg tgaacgggcg cagttccgtg ccgagttgct cgccgacatc
     5101 aactcggatc tgcgctcctt cggcatgaag gtcgtatcgg tgtcgctcca gaagatctgg
     5161 gatacttcga actacatagc caacctggcc cagaagacgc tggccgagaa gcgccaggcc
     5221 gtcgagatcg aggaatcgcg gctacgcgcc atagcggacc aggccgagag cgaggcgcgt
     5281 aaacgcatcg aggtggccag gagccaggcc gacgaggcga tcatcagcgc tcgccaggaa
     5341 ctcgaggtgt accgccgcga gtcgtccggc ctgatcgagg aggccaagct caaggccgat
     5401 caggcaatag ccgaggcgcg caactccggc gagtcggcgg tgcaggccca gctcgtcgag
     5461 ctacagaagc tacgcaacca gtcctcggta acgctggtcg cggccgcccg ggaggaagcc
     5521 gccaggatac tcgccgaggg cgagc
//
LOCUS       DAOPHV010000108         5520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_19480_length_5520_cov_4.160293, whole genome shotgun sequence.
ACCESSION   DAOPHV010000108 DAOPHV010000000
VERSION     DAOPHV010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19480_length_5520_cov_4.160293"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="PLQ29_04200"
     CDS             <1..1225
                     /locus_tag="PLQ29_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG85878.1"
                     /translation="QAKQHAHALEAKDTDTAELERLEKDIAIKNSDLGKRQEELKKKI
                     DKLGSLQTELERLHLGLAQTETEIRNVQDNFREQYGRELVEFEERMYELRSQAAELRD
                     RLSDRKQRLKDLGSVNLMAPEEYTEVKERYEFLSTQLADLQKARDDLRRITQEIRDES
                     AEMFLNTYNRVKKNFHNMFRRLFGGGRGELRLSDPDHVLESGIEIFAQPPGKKLENIT
                     LLSGGEKSLTAIALLFATYMVRPSPFCFLDEIDAALDEQNVIRFVNLLREFGRTSQFI
                     VITHNKKTVTCADALLGVTMEESGVTKVIAVRLERSDGQPITMPAPSEPFIDEDVEYE
                     AGRELTLEPEAAKPEKARAKADEATARASDHEETDVVSEAVAIVATTDDTVADVEAIR
                     DDGLQASGDAGTTEP"
     gene            1222..1725
                     /locus_tag="PLQ29_04205"
     CDS             1222..1725
                     /locus_tag="PLQ29_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85879.1"
                     /translation="MRFPPALAARLGAVAKPALGLARAARAAVGKALAALAEKLRGLS
                     PRGKAIARLSVVGCLAFATAIALIAMTAGSNARRDGARLAPSAEPSVASVRSEPTVPG
                     SGPALASMLLIPGDGERPWPLALEPKPRYTDADAAELSPDLGAVEIGDLSRRRKVALE
                     TIYGAVH"
     gene            1733..3190
                     /locus_tag="PLQ29_04210"
     CDS             1733..3190
                     /locus_tag="PLQ29_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85880.1"
                     /translation="MIAAALATLSAFSCAGGPEPLAADEAVVAAEPIAASDVPALATE
                     RLSPEVLTARRPVAYPGAVILPTLPEPALPVPVAPSTSPETIQAASARPDTGSTGPVV
                     DPGIPSDVTVLSTAPSYGTISLAEPEIPEPAPTKETAPVAPVAPVAAAKPVAPVAASK
                     PAAPKPAVPKPAAPEAAAPEASPVVPAAKATPAPEPTKAPEPEASAEPVEPAYVLPAA
                     PTSSARSVAAAAVPVAETRLETARGQGFELRFPGSGWVYLGDEDGMDGIKYNARRFED
                     SQAVFSLNPERSGEYLLRFHRQNPIDGSTEVSLVRVVVTDKAAMVPASVPASVPASVP
                     ASVPATASAASTATVPTAPATSAPVASTPSASSRVSTPTVPAAPAPEAPAPASSALSD
                     PEALLRLARDELDAKRVQSAIEALDRYLSLFPYGGDEAYYLYALACEQDTPFRDIKRS
                     YEYYKRVRDQYPRSPRWKAAAERVAYLERHYFGLR"
     gene            3256..4473
                     /locus_tag="PLQ29_04215"
     CDS             3256..4473
                     /locus_tag="PLQ29_04215"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HPG85881.1"
                     /translation="MATIRRYYERPAIAELDSVVTGVSERADGASVVTLSETIFYPEG
                     GGQPCDLGDLAGLPLEAALEEGDEVAHILRADPVALAAAGVCPGATVRSVVDLGRRID
                     HSEQHTAQHLLSSILLRAMGARTLSFHLGERHSFIDLNIAPFGRDDADAIEDEVTRAA
                     LDDYGVTTHLCPPEDPASFPLRKEPSVEAAVLRVIEIDGIEYSACCGTHVASTRSLEP
                     FRILRAEKYKGGCRVYFVAGGRARADYRRVAGIAVRAAAAAGCADDELEAAVRSSRER
                     QKELERSLAVALDEVAAARAEALDGSGDRSGDDSPAEPVFAVAASYDAGNRLARALAR
                     RGRVAVVDCGDELKVVACSPTGGPAVDAAYSQAAMAAGGKGGGGKAYFQAAFPDRASL
                     EAFVAAARAAVVS"
     gene            complement(4476..>5520)
                     /locus_tag="PLQ29_04220"
     CDS             complement(4476..>5520)
                     /locus_tag="PLQ29_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPG85882.1"
                     /translation="DHFDFSKPGRRILVIGPMGSGKTEYSARVWRDSRVALRKGERVA
                     ARTTTAGADRRRMEFVRFGIDQDRFKDYPDDALPYRGGYERCGDSIGCAADSFGLEAL
                     IAERPGSGTWILDEASFYDERVAYLIRNESESRGLVFILPTLVLNFRKEIFNQAARLL
                     LENATDVFPLTAYCEHPDCIADSLYTYRYYSVEGRECPALYFDPLIIVGGDRLKDDPR
                     EPNYCTRCDEHHYLPGKEYTFFTLKPLGEAASRGDLKPLLAELAAIAGDVERSRLYAS
                     LKRLYLDGPRPRPENMAALEVPCIAEKALCYLFAEQNLLSAEQLRRVVADLGLDRDYL
                     RKRLSDNRRPLEP"
BASE COUNT          931 a         1836 c         1871 g          882 t
ORIGIN      
        1 gcaagccaag cagcacgcgc acgcgctgga ggccaaggac accgacaccg ccgaactcga
       61 gcgcctcgag aaggatatag ccatcaagaa cagcgacctc ggcaagcggc aggaggagct
      121 gaagaagaag atagacaagc tcggttcgtt gcagacggag ctcgagcggc tgcacctcgg
      181 cctggcccag accgagaccg agatacgcaa cgtccaggac aacttccgcg agcagtacgg
      241 ccgcgagctc gtcgagttcg aggagcggat gtacgagctg cgctcccagg ccgccgagct
      301 ccgcgacagg ctgtccgacc ggaagcagcg gctcaaggac ctcggctcgg tcaacttgat
      361 ggcgccggag gagtataccg aggtcaagga acgctacgag ttcctgtcga ctcagctcgc
      421 cgacctccag aaggcccgcg acgacctcag gcgcatcacc caggagatac gcgacgagag
      481 cgcggaaatg ttcctgaata cctacaaccg cgtcaagaag aacttccata acatgttcag
      541 gcgcctcttt ggcggcggcc gaggcgagct acgcctgagc gacccggacc acgtactcga
      601 gtctggcatc gagattttcg cgcagccgcc cgggaagaag ctcgagaaca tcacgctgct
      661 gtcgggcggc gagaagagcc tgaccgccat cgcgctgctg ttcgccacct acatggtcag
      721 gccgtcgccg ttctgcttcc tggacgaaat cgacgcggcg ctcgacgagc agaatgtcat
      781 ccgcttcgtc aacctgctgc gggaattcgg ccgtacgagc cagttcatcg tcatcaccca
      841 caacaagaag acggtcacct gcgccgacgc cctgctcggc gtgacgatgg aggagtccgg
      901 cgtgaccaag gtgatagccg tcaggctgga gcgctccgac ggccagccca tcacgatgcc
      961 cgctccgtcc gaacccttca tcgacgagga cgtcgagtac gaagctggtc gcgaactgac
     1021 cctggaacct gaggccgcca aacctgagaa ggccagggcc aaggccgacg aggcgaccgc
     1081 gcgagcttcc gaccacgagg agaccgatgt cgtatccgag gccgtcgcta tcgtcgccac
     1141 gacagacgac acggtggccg acgtcgaagc gatccgcgat gacggccttc aggcctcagg
     1201 cgacgcgggg acgacggaac catgaggttt ccgccggccc tggcggctcg gttaggtgcc
     1261 gtagccaagc cggcgctagg cctcgcgcga gcggcgcgcg cggccgtcgg caaggcgctg
     1321 gcggcgttgg ccgagaagct gcgcggcctg tcgcctcggg gtaaggccat cgcgcgcctc
     1381 tccgtcgtcg gctgcctggc gttcgctacg gccatcgcgc tcatagcgat gacggccggg
     1441 tcgaacgctc gccgagacgg cgctcgactc gcgccgtccg cggaaccgag tgtcgcttcg
     1501 gtacggtcgg agccgaccgt gccgggctcc ggcccggccc tcgcgtcgat gctcctgatt
     1561 ccaggcgacg gcgaacgccc ctggccgctg gcgctcgagc cgaagccgag gtatactgac
     1621 gcggacgcgg cggaactgag cccggaccta ggcgccgtcg aaatcggcga cttgagccgt
     1681 cgcaggaagg tagcacttga aacgatctac ggcgcggtcc actagcggct acatgatagc
     1741 tgcggcctta gcgaccctga gcgcgttctc gtgcgcgggc ggcccggaac cgctggcggc
     1801 ggacgaggcc gtcgtggcgg ctgaaccgat cgctgcctcg gacgttccgg ccctggctac
     1861 cgagcgtctt tcgcccgagg tcctgaccgc tagacggccc gtcgcctatc cgggcgccgt
     1921 gatcctgccc accctgcctg aaccagcgct acccgtacca gtagcgccat ctacctcgcc
     1981 cgagacgatc caggcggcct cggcgaggcc ggataccggc tcgaccggac cggtcgtcga
     2041 tccaggcata ccaagcgacg tcacggtgct gtcgacggca ccgtcgtacg gtaccatcag
     2101 cctagccgag ccggagatac cggaacccgc cccgacgaag gaaaccgccc cggtcgctcc
     2161 ggtcgctccg gtcgccgccg cgaagcccgt ggccccggtg gctgcctcga agcccgccgc
     2221 gcccaaacct gccgtgccca aaccagccgc gcccgaggca gccgcgcccg aggcatcgcc
     2281 ggtcgtgccc gctgcgaagg cgacccccgc tcccgagccg accaaggctc ccgaaccgga
     2341 agcgtcggcc gagcctgtgg aacccgctta cgtactgccg gccgcgccaa cgtcctcggc
     2401 cagatcggtc gccgcggctg ccgtaccggt cgccgagacc aggctcgaga ccgcgagagg
     2461 tcagggcttc gagctacgct ttcctggctc cggttgggtc tatctcggcg acgaggacgg
     2521 gatggacggt atcaagtata acgccaggcg attcgaagat tcgcaggcgg tgttctctct
     2581 gaacccggag cgttcgggcg agtatctcct acgcttccat cggcagaatc cgatcgacgg
     2641 ctcgaccgaa gtcagcctcg tacgcgtcgt cgtgaccgac aaggcggcta tggttccggc
     2701 ttccgttccg gcttccgttc cggcttccgt tccggcttcc gttccggcta ccgcatcagc
     2761 cgcatctacc gctaccgttc ccaccgctcc agcgacaagc gctcctgtag caagcactcc
     2821 ctcggcgtcg tctcgagtct cgactccgac tgttcccgcg gctcccgctc ccgaagctcc
     2881 cgctcccgct tcatccgcgc tgagcgatcc agaggctttg ctcaggctgg cccgcgacga
     2941 gctcgacgca aagcgggtgc agtccgcgat cgaggcgctc gatcgctact tgtcattgtt
     3001 cccgtacgga ggcgacgagg cctactactt gtacgcgctc gcctgcgagc aggacacgcc
     3061 gtttagggat atcaagcgat catacgagta ctataagcgt gtgcgcgacc agtacccgag
     3121 gagcccgcgc tggaaagcgg cggccgagcg cgtcgcctat ctcgaacggc actatttcgg
     3181 cctgcggtag cgggcgtccg cccgacgcgt ggtcgcgtct agtgttacga accttgttta
     3241 ggatatgctc gatccatggc aaccatcagg cgatactacg agcgaccggc catcgcggag
     3301 ctcgactcgg tcgtaaccgg agtgagcgag cgggccgacg gcgccagcgt cgtgaccctg
     3361 tcggagacga tcttctatcc cgaaggcgga ggacagccct gcgacctcgg cgacctggcc
     3421 ggactgccgc tggaggccgc gctcgaggag ggcgacgaag tcgcgcatat cctacgcgcc
     3481 gatcccgtcg cgctagccgc ggctggcgtc tgccccggcg ccacggttcg gtccgtcgta
     3541 gaccttgggc gaaggatcga ccatagcgaa caacatacgg ctcagcacct cctgtcgtcg
     3601 atactcctgc gcgcgatggg tgcgaggacc ttgtcgttcc acctaggcga gcggcactcc
     3661 ttcatagatc tgaatatcgc tccgttcgga agggacgacg ccgacgcgat cgaggacgag
     3721 gtgacgcgcg cggccctcga cgactatggg gtgactacgc acctatgccc gcccgaggat
     3781 ccggcgtcct tcccgctacg caaggagccg tcagtcgagg cggcggtgtt gcgtgtgatc
     3841 gagatagatg gcatcgagta ctcggcctgt tgcggcaccc atgtagcgtc gacgagatcg
     3901 ctcgagccgt tcaggattct tagggcggag aagtacaagg gcggctgccg ggtctacttc
     3961 gtggccggcg gcagggccag ggcggactat aggcgcgtcg cggggatagc ggttcgggcg
     4021 gccgccgcgg ccggctgcgc cgatgacgag ctcgaggcgg ccgtcaggtc gtctagggag
     4081 cgacagaagg agctggagcg ttccctggcg gtggcgctcg acgaggtcgc ggcggccaga
     4141 gccgaggcgc tcgacgggtc gggcgacagg tcgggcgacg attcgccggc cgagccggtc
     4201 ttcgcggtgg cggccagcta tgacgcgggc aaccggctgg ccagagcgct cgccaggcgc
     4261 ggccgggtag ccgtggtcga ctgcggcgac gagctcaagg tcgtggcctg ctcgccgacc
     4321 ggcggtccgg ccgtcgatgc ggcttatagc caggcggcca tggcggccgg gggcaagggc
     4381 ggcggcggca aggcttattt ccaggcggcc ttcccggatc gcgcatcgct cgaggccttc
     4441 gtcgcggctg cccgggccgc tgtcgtcagc tagcgctacg gctccagcgg gcggcgattg
     4501 tccgatagcc gcttgcgcag gtagtcgcgg tcgagcccga ggtcggcgac gaccctgcgt
     4561 agttgttccg ccgacaagag gttctgttcg gcgaataggt agcacagcgc cttctccgcg
     4621 atacacggta cttcgagcgc tgccatgttc tccggcctag gtcgcggtcc gtcgaggtag
     4681 agccgcttga ggctggcgta gagcctggat cgctcgacgt cgcccgctat cgcggcaagc
     4741 tcggcgagca ggggcttgag gtcgccgcgc gaggcggcct cgccgagcgg cttgagcgtg
     4801 aagaaggtgt attccttgcc cggcaggtag tggtgttcgt cacagcgcgt gcagtagttc
     4861 ggttcgcgcg ggtcgtcctt gagccggtcg ccgccgacga tgatcagcgg gtcgaagtat
     4921 agcgcggggc actcgcgccc ctcgacacta tagtagcggt aggtgtacag cgagtcggct
     4981 atgcagtcgg gatgctcgca gtatgcggtc agcgggaaca cgtcggtcgc gttctcgagg
     5041 agtagccgcg ccgcctggtt gaatatctct ttgcggaagt tgagcacgag cgtcggcagg
     5101 atgaacacga ggccgcgcga ttcggactcg ttgcgtatca ggtaggctac gcgttcgtcg
     5161 tagaaggatg cctcgtcgag tatccaggtg cccgagccgg gacgctcggc tatcagcgcc
     5221 tcgagcccga acgagtcggc cgcgcagcct atcgagtcgc cgcaacgctc gtagccgccg
     5281 cggtagggca gggcatcgtc cgggtagtct ttgaagcggt cctggtcgat gccgaagcgt
     5341 acgaactcca tgcgccgccg gtcggccccg gcggtcgtcg tgcgcgcggc tacccgctcg
     5401 cccttgcgca gggccacgcg ggagtcgcgc cagactctgg cgctgtactc ggtcttgccc
     5461 gagcccatcg gcccgatcac gaggatgcgg cggccgggct tggagaagtc gaagtggtcg
//
LOCUS       DAOPHV010000109         5518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_19495_length_5518_cov_4.719019, whole genome shotgun sequence.
ACCESSION   DAOPHV010000109 DAOPHV010000000
VERSION     DAOPHV010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19495_length_5518_cov_4.719019"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..145
                     /locus_tag="PLQ29_04225"
     CDS             11..145
                     /locus_tag="PLQ29_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HPG85883.1"
                     /translation="MADFPVVNEVYGARFKGDKPARSTVAVAGLPKGGLVEIEAILSL
                     "
     gene            149..1321
                     /locus_tag="PLQ29_04230"
     CDS             149..1321
                     /locus_tag="PLQ29_04230"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HPG85884.1"
                     /translation="MYDFDSPIDRRGTDSVKWMAGGACHSSIWPDVIPMWVADMDFAS
                     PPAVALAIRRRAEHPVYGYSMKTEAFFHSYLDWMGSRHGLAIEREWLSFSPGIVPAIA
                     TAVRALTEPGDGVAIMPPVYHPFRNMVEKNGRIVVEAPLSRSDGRYGIDLGALDAACS
                     RSRLLVLCSPHNPVGRVWTRDELEAVFDIAERRDVIVFSDEIHGDLVYDDGRMVPSLA
                     LGGGATDRLIAAWAPSKTFNIAGLQASFIVTPDPAIRARFDAESEATGIGSPNCLAQD
                     AVIAAYREGSAWLDEALRHMRGNYDALVSGLEARVPGIRAYPLEGTYLAWLDFRGVGL
                     QGDVGAEIVERAGLWLDAGLRFGSAGAGFARMNLGCPRRTVETAVERLAAAFGPGA"
     gene            complement(1350..2129)
                     /locus_tag="PLQ29_04235"
     CDS             complement(1350..2129)
                     /locus_tag="PLQ29_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85885.1"
                     /translation="MGSDSATVAEALRMALATATFFVGLAGAAGLRSILRHGDGGGTR
                     RANAAMAAASVAVAIAAWGFVTATRAAVAAPAGLSWYVAAGGVAGLLVGMFPRAAGVP
                     LALAAFASAALLSAALSGWLPWEPGAEAASITVYSADSGGSSITLRYAVPGGRMVEQD
                     LSFGPGDLWLTFELVDIRGPASLAYGRRRYRLAGASDGGDGAIIKGDRGPLLDAAGGR
                     RLAGALGCSVAELEAPPFEPEPLAVVAYLLHDDGAVVMKER"
     gene            complement(2119..3636)
                     /locus_tag="PLQ29_04240"
     CDS             complement(2119..3636)
                     /locus_tag="PLQ29_04240"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HPG85886.1"
                     /translation="MKPIERLRTLDAEAKLVEHIGAALGWDQETYMPAGAIDERADQL
                     AYVETLAHEKATAPEIGRLLDELGSTGAEPSGPVSLPTIDRAYLRVMRRRYDQATKLP
                     AELVSEMARATSLSQAAWVDARKHNDFKAFAPHLSKMLEYNKKVAACLDPSRKPYDVL
                     LDRFEEGASEEQVASIFGPLREELVSLLDRIRGKPQIDDSFLRRRCPAAAQAEASAYF
                     MRALAYDEKRGRLDTTAHPFTTTLGESDVRITTRYLEDYFPSSLFSTIHESGHALYEM
                     GIDPAPEYRRTSLAEASSMAVHESQSRLWENTVGRSLGFWLQHLPALRQIVAPVLDGV
                     GLEEFYRAINKVEPSLIRTEADEVTYGLHVILRFELEGRMLSGDLAVDELPAAWNDGM
                     RRLLGVTPSNDAEGCLQDIHWPMGSFGYFPSYALGNLYGAQWWDAMKAQGLDPKAAVE
                     LGDLGSILSWLRSNVHRCGSMYRPGELVERVTGAPLDPSHFVRYLNDKYAGVYGL"
     gene            3762..>5518
                     /locus_tag="PLQ29_04245"
     CDS             3762..>5518
                     /locus_tag="PLQ29_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85887.1"
                     /translation="MPTIDGSGDGAAATVDDGKGRKGRRPLRAVAWVLGIVVGAPALA
                     AAGLLVVSVLSRSDPAAHMPPGFQAYASLPSAGTFVREALDLKALDAILSGPELGAAR
                     GLVRSLRAQPFLRTAAFDRLADLRVDAALYEGGGFVAAASLGYRSAALRLAPLAARLV
                     PGLLAKVPGLSWVPEATPSRFELKAGEATIYAVRYRDALVVASSVELLAAAREPTGRG
                     VDPALAAALAEPSSGSLRFLVDPATLTAGLASGGGLAAGLLSALEFPSLAVVDLRLAN
                     ERVELSVNLTSTVRDSGLAALFGRRSRTPSILSRFPESASYFTLLAAGEPSGLWKALS
                     PALGDSVVSAFDTADRASKLALGLGLDALLFSWMGDELGVYGSSLGSEPVFFARIADE
                     KARRRVFDTIFGSLLVGRDVSAMVGDTRVPRIVFPPFLRSFLEQLGVGLVEPFYLVED
                     GFLYVCQSAELLAAAVAETRDGRLLVKTDRWKDGAAGVSPESSASLFYSLDRSVPFFM
                     RGNAGLASALKLYGRGVASVRLSPGSVRLELSAVPAAKGVKATLPGFPIDAGGRMGSD
                     PVLGRTASGVPVAFWTSGQ"
BASE COUNT          753 a         1913 c         1944 g          908 t
ORIGIN      
        1 cctcaaggat atggccgact tcccggtggt caacgaggtc tacggcgccc gcttcaaggg
       61 tgacaagccc gcccgatcga ccgtcgcggt cgcgggctta cccaagggcg ggctcgtgga
      121 gatcgaggcg atactgtcgc tgtaggccat gtacgacttc gatagcccga tagaccggag
      181 aggcaccgac tcggtcaagt ggatggccgg cggcgcctgc cattcgtcga tctggcccga
      241 cgtgatcccg atgtgggtgg ccgacatgga tttcgcctcg ccgccggccg tcgccctggc
      301 gatcaggcgg agggccgagc atccggtcta cggctactcg atgaagaccg aggcgttctt
      361 ccattcgtac ctcgactgga tgggctcgcg ccacggctta gccatcgagc gcgagtggct
      421 gtccttctct cctggcatcg tcccggcgat cgcgacggcc gttcgagcgc tgaccgagcc
      481 gggcgacggc gtcgccatca tgccgccggt ctaccacccg ttcaggaaca tggtcgagaa
      541 gaacggccgg atcgtcgtcg aggcccctct ctccaggtcc gacggccgct acggtatcga
      601 cctgggagcc ttggacgcgg cttgctcccg ttcgaggctc ctcgtgctct gtagcccgca
      661 caacccggtc gggcgcgttt ggacgaggga cgagctcgag gccgtattcg acatagccga
      721 gcgccgcgac gtgatcgtgt tctccgacga gatccatggc gacctggtct acgacgacgg
      781 gcgcatggtg ccgtcgctcg ccctgggcgg cggcgcgact gatcgcctga tcgccgcctg
      841 ggcgccgagc aagactttca atattgccgg cctccaggcg tcgttcatcg tcacgcccga
      901 tccggccatc cgcgctcgat tcgacgccga gagcgaggcc acgggcattg gctcgccgaa
      961 ctgcctggcc caggacgcgg tcatagcggc gtaccgcgag ggctcggcct ggctcgatga
     1021 ggcgctccgc catatgcgcg gcaactatga cgccctcgta tccggcctcg aggcccgcgt
     1081 tcccggcata cgggcctatc cgctcgaggg cacgtacctg gcctggctgg atttcagggg
     1141 cgtcgggctt cagggcgacg tcggcgcgga gatcgtcgag cgggccggcc tgtggctgga
     1201 tgcgggcctc cgcttcggtt cggccggcgc cggcttcgct cgtatgaacc tcggctgccc
     1261 gaggcgcacg gtcgagacgg ccgtggaacg cctggccgcg gccttcggac ccggcgccta
     1321 gcgcctccgg cccggccgac gccgggcctc tagcgctctt tcatgacgac ggcgccgtcg
     1381 tcgtgcagca ggtaggcgac gacggccagg ggctccggct cgaagggcgg cgcttccagc
     1441 tcggccaccg agcagccgag cgccccggcc agccgtcgcc cgcccgcagc gtcgaggagc
     1501 ggcccgcggt cgcccttgat gatcgccccg tcgccgccgt ccgacgcccc ggcgagcctg
     1561 tagcgccgcc ggccgtaggc gagcgaggcg gggccgcgta tgtcgaccag ctcgaaggtc
     1621 agccagaggt cgcccggccc aaaggacagg tcctgttcga ccatcctgcc gccgggaacg
     1681 gcgtatcgta gcgttatcga cgagccgccc gagtcggccg aatacaccgt gatcgaggcg
     1741 gcctccgctc ccggctccca gggcagccaa cccgatagtg ccgccgacag cagggcggcc
     1801 gacgcgaacg cggccaaggc gagcggaacg ccggccgccc tcgggaacat cccgacgagg
     1861 aggccagcta cgccgccagc ggctacgtac caggagagtc cggcgggcgc ggcgacggcg
     1921 gccctcgtcg ccgtgacgaa gccccaggcc gcgatggcga cggccacgct agcggcggcc
     1981 atcgcggcgt tggcccggcg cgtaccgccg ccgtcgccgt gcctcaggat cgagcgcaaa
     2041 ccggccgcgc ccgccaatcc gacgaagaac gtggccgtcg cgagggccat gcgcagcgcc
     2101 tctgcgaccg ttgcggaatc agagcccata gaccccagcg tacttgtcgt tcaggtagcg
     2161 cacgaagtgc gacgggtcga gcggggctcc ggtgacccgc tccacgagct cgccgggcct
     2221 gtacatcgag ccgcagcggt gtacgttcga cctgagccag gataggatcg aaccgaggtc
     2281 gccgagctcg acggccgcct tcgggtcgag tccctgggcc ttcatcgcgt cccaccactg
     2341 ggcgccatag aggttgccca gggcgtagct cgggaagtag ccgaaggagc ccatcggcca
     2401 gtgtatgtcc tggaggcagc cctcggcgtc gttcgacggc gtgacgccga gcaggcgccg
     2461 catgccgtcg ttccaggccg ccggcagctc gtcgaccgcc aggtcgcccg atagcatccg
     2521 tccttcgagc tcgaagcgca ggatcacgtg gaggccgtag gtcacctcgt cggcctcggt
     2581 gcgaatcagc gacggctcga ccttgttgat ggcgcggtag aactcctcga gcccgacgcc
     2641 gtcgaggacg ggtgcgacga tctgcctgag agccggcagg tgctgtagcc agaagccgag
     2701 cgagcggccg acggtgtttt cccagagccg cgactgcgac tcgtggacgg ccatgctgga
     2761 agcctcggcg agcgaggtgc ggcggtactc cggcgccggg tctatgccca tctcgtatag
     2821 cgcgtgcccg ctctcgtgga tggtgctgaa caggctcgac gggaagtaat cctcgaggta
     2881 gcgcgtggtg atgcgtacgt ccgactcgcc gagcgtggtc gtgaacggat gggccgtggt
     2941 gtcgagccga ccacgcttct cgtcgtaggc cagcgcgcgc atgaagtacg cgctcgcctc
     3001 cgcctgggcg gcggccgggc agcgccggcg caggaacgag tcgtcgatct gcggcttgcc
     3061 gcgtatccta tcgagcaggg agacgagctc ctcgcgtagc ggcccgaaga tcgaggcgac
     3121 ctgttcctcg ctagcgcctt cctcgaagcg gtcgagtagc acgtcgtagg gcttcctgga
     3181 cggatcgagg caggcggcca ccttcttgtt atattcgagc atcttggata ggtgcggggc
     3241 gaaggctttg aagtcgttat gcttcctggc gtcgacccag gccgcctggg acaggctcgt
     3301 cgccctggcc atctccgaga cgagctcggc cggcagcttg gtcgcctggt cgtaccggcg
     3361 ccgcataacg cgtaggtagg ctcggtctat cgtcggcagg ctcacggggc cggacggctc
     3421 ggcccccgtc gaacccagct cgtcgagcag gcggcctatc tccggcgcgg tggccttctc
     3481 gtgcgccagc gtctcgacgt aggcgagctg gtcggccctc tcgtcgatgg cgcccgcggg
     3541 catgtaggtt tcctgatccc agccgagcgc cgcgccgatg tgctccacga gcttggcctc
     3601 ggcgtcgagg gtccgtagtc gttctatagg cttcatatac gctcctcttt ccgtaccgtg
     3661 catatcagcg aggtgatgat cgcacgcggc gggcgttgac gcaaccgcgg gtttcggtcg
     3721 acaatcggcg ggcttgccag taccatgttc cggaggacga cgtgccaaca atcgacggat
     3781 cgggagacgg agcggccgcg acggtcgatg atggaaaggg gcggaagggg cgaaggccgc
     3841 tacgggccgt ggcctgggta ctcgggatcg tcgtcggcgc gcccgcgttg gccgccgccg
     3901 gcttgctcgt cgtttcggtc ctgtcgagga gcgatccggc cgcccacatg cctcccggct
     3961 tccaggccta cgcgagcctg ccttcggccg ggaccttcgt ccgcgaggcc ctggacctca
     4021 aggcgctcga cgcgatcctg tcggggccgg aactcggcgc ggcgcggggc ctcgtgcgct
     4081 cgctgcgggc ccagcccttc ctgcggacgg ccgccttcga ccgcctggcc gacctcaggg
     4141 tggacgccgc gctctacgaa ggcggcggct tcgtggcggc ggcctcgctc gggtaccggt
     4201 cggcggcgct acgcctggcg cccctggccg cgaggctagt cccgggcctg ctggccaagg
     4261 ttcccggcct gtcgtgggtg cccgaggcga cgccgtcgcg cttcgagctc aaggcgggcg
     4321 aggcgacgat ctacgccgtc aggtaccgcg acgcgctcgt cgtcgcctcg agcgtagaat
     4381 tattggccgc ggcccgcgag ccgaccggcc gcggcgtcga cccggccctg gcggcggccc
     4441 tggccgagcc gtcatcgggt tcgttgcgct tcctcgtcga tccggcgacg ctgaccgccg
     4501 gcctggcctc gggaggcgga ctggccgccg ggctcctgtc cgcgctcgag ttcccgtcgc
     4561 tcgccgtcgt cgacctgagg ctcgcgaacg agcgcgtcga gctgtccgtc aacctgactt
     4621 cgaccgtacg cgactccggc ctggcggcct tgttcgggcg ccgctccagg acgccgtcga
     4681 tcctgtcccg tttccccgaa tcagcctcgt acttcacgct actggcggcc ggcgagccgt
     4741 ccgggctgtg gaaggccctg tccccggcgc tcggcgactc ggtcgtatcc gccttcgata
     4801 ccgccgacag ggcctcgaaa ctggcgctcg gcctcggcct ggacgccctc ctgttctcgt
     4861 ggatgggcga cgagctgggc gtgtacggta gctcgctcgg ctcggaaccg gtcttcttcg
     4921 cgcggatcgc cgacgagaag gcgcgcaggc gggtcttcga taccatcttc ggctcgctcc
     4981 tggtcggccg cgacgtgtcg gcgatggtcg gcgatacccg cgtaccacgc atcgtgttcc
     5041 ctccgttcct gcggtcgttc ctggagcagc tcggcgtcgg cctcgtcgaa ccgttctacc
     5101 tggtcgagga cggcttcctg tacgtctgcc agagcgccga gctgctagcc gccgcggtcg
     5161 ccgagacgag ggacggccgg ctcctggtca agacggaccg gtggaaggac ggagcggccg
     5221 gcgtctcgcc cgagtcgtcg gcctcgctgt tctattcgct cgaccggagc gttccgttct
     5281 tcatgcgcgg caacgcgggg ctcgcctcgg cgctcaagct gtacgggcgg ggggtcgcct
     5341 cggtccgact atcgcccggt tccgtcaggc tcgagctgtc ggcggtaccg gccgccaagg
     5401 gcgttaaggc gacgctgccg ggcttcccta tcgacgcagg cggcaggatg ggctcggacc
     5461 cggtcttagg ccggacggcc tccggcgtgc cggtggcctt ctggacgagc ggccagaa
//
LOCUS       DAOPHV010000110         5517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_19501_length_5517_cov_4.331197, whole genome shotgun sequence.
ACCESSION   DAOPHV010000110 DAOPHV010000000
VERSION     DAOPHV010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19501_length_5517_cov_4.331197"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PLQ29_04250"
     CDS             <1..183
                     /locus_tag="PLQ29_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OST-HTH/LOTUS domain-containing protein"
                     /protein_id="HPG85888.1"
                     /translation="AYLGPVGQYVANRQPDFDPRNYGFRKLAELIRSCGWFEVEERSS
                     PTDTGKQIYIRKRQGR"
     gene            complement(190..2412)
                     /locus_tag="PLQ29_04255"
     CDS             complement(190..2412)
                     /locus_tag="PLQ29_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85889.1"
                     /translation="MLGFGADGASLDALTAAGARLWEGCSQYVRLGKAPLRSTIDPGA
                     ALRRLVADFSSAGRYDEFRYLIEYVERSVASGRLVIGSVDDVPPLPGFWPARVGAAKR
                     AIVVNADELASLSQDDAVYRSSFLRAAAALYYADASPAVPGVHSGRNAAAELVAMMSG
                     LYVESIYLLEVLRLAEPEGTAIDYIDLLLGSAVYDNLRGAASALFRVDLDYAYDAVNA
                     ATKAASARGLADYLERAARELVELEAIISTASLRASPGREDLEPAGRARAALIALPWA
                     AREAAQGSYGSDSSVRRALAGLVEALSSMASAYAPLDPLAMEETRRFLNDLRFRAPNP
                     LAPVVGEPLPDYVEPVGPSGPPAFPGSAEERWYRLCLYDNPYEPVGELPIDFEAAMLG
                     AAYRFSYGSEGGLLAIEHFIYGRLRPSDLLQWAARIRTGRDATGAWSVWESSYGYPMM
                     NEAGYATRRESRTRNGLSLSFYATDGRRVPDCSNTYIVGIEPRNGGYDLSYRTADGRL
                     WSGYYGYALDSRRIAQGGEETRSHFDELGRPVVTLSLGFHRSVSVKERRGGALVVDMA
                     FFDEAGAPAITFEGYYRQRVRLDEEGRTYELLDADGEPMTSCLGWAAERIDWDRGLPV
                     RLAFSAPDGGAVISMEGFHEARYELSPDGSIVGLSFYGTEGEPRSSYEGYHRREGEYD
                     DKGALIGVEYFAADGSPSSDRSGASRIRWYLGADGSVESVRVWDAHGALIAGEPSRAL
                     "
     gene            complement(2482..4449)
                     /locus_tag="PLQ29_04260"
     CDS             complement(2482..4449)
                     /locus_tag="PLQ29_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85890.1"
                     /translation="MPIWSDIERALTPSREDTALAADLAAAIDRGLPLAITQSVLIAA
                     SDAAMASLYATLEARSSPPEPRRLRSDSSWMLESDFCWINVRACASGSLPGTFIRAAK
                     LLPAIASDAILLAPFHPTQFDLCYAPETMTMADPTLFDRELVGAGVSAENQLRAFVSA
                     CHLLGKCVGYELLPYAAQFSRIAMERPQLFRWVALDDERRGLAHADPAFPYRSEDRLR
                     DAEQVSDIVATVKEDYGIRSFRVIEGDAPEVVDAKVKAYYSAIRLCADRGLWPVPAHA
                     RAGVGVPSFLRCDSSDDFPIFSYRDIDGADIGEDAYSVVAPFAFYDDMPPNSIPTEPV
                     RRNDEAIGYYAEVFSYWRDSFGFDFVRYNAADRVFSESVDDEGSVPSCDRPTSEVVAQ
                     AIRASRDGAPGVGAISPSKGDETGAFGALGFDLLMGGEALRRVDAPLLRAAFSLYDSL
                     AAERTGGARTSVCFAVDLPESSAPRLWGSPLRRVMGPERMRLRHVAARFLSVGAGRRP
                     MFETMGFQDGSTGLYEAGLSVRGLDWADDAVFAAGYAAIERFYVGLRPFLASAAIIDR
                     SVDDGLAWWQIRAGDSARLVVVALSLETAEGTAPGRITIPIGTALGELSGTAYRLPDL
                     AGAPIDAAGSLVVELGFLDSVVVDLVPAYYR"
     gene            complement(4456..>5517)
                     /locus_tag="PLQ29_04265"
     CDS             complement(4456..>5517)
                     /locus_tag="PLQ29_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPG85891.1"
                     /translation="SLEASVVCSFTALDEYFGVSPAPPRFERFDGDIVGLAKATDGLS
                     FPGLPYAEAVGGGPASRVLYRCSDGEAGAYPQTALTRLPAKRSFDARGGVYESLSEPT
                     PSPRQASPELILFEAAALAARYRYEPDPSYLPETPSGLPVEYQRDLLDLVVTGARPDK
                     GFELLRRSGFVDEYWPEVAELGSVSQAKDYHPEGDAWRHTMETFGHRKAPDLVLSLAL
                     LLHDTGKPDAVPAGGRRFDGHSELGERAARRLLGRLGYPGDVVDSVAFLVRYHMLPAA
                     LPRIPPSSVGRALDSPLFPTLLELYRCDELSTFRGPDGYYEACAAYKAYLKNARNPYR
                     DDDGKRRGDSLHRARPRTL"
BASE COUNT          941 a         1868 c         1934 g          774 t
ORIGIN      
        1 gcctatctcg ggccggtcgg ccagtacgtg gccaaccggc aaccggactt cgacccgcgc
       61 aactacggct tccgcaagct ggccgagctg atacgctcct gcggctggtt cgaggtcgag
      121 gagcgttcca gcccgaccga caccggcaag cagatctata tccgcaagcg ccagggacgc
      181 tagcctcgct tacagcgccc tcgacggctc tcccgctatc agggcgccat gcgcgtccca
      241 gacccgcacc gactcgacgg agccgtccgc gccgaggtac catcgtatcc tcgaggcgcc
      301 gctcctgtcg gacgagggcg agccgtcggc cgcgaagtac tcgacgccga tcagggcgcc
      361 cttatcgtcg tactcgcctt cacgtcggtg atagccctcg taggacgaac gtggctcgcc
      421 ctcggtgccg tagaacgaca ggccgacgat ggagccatcg ggcgacaact cgtagcgagc
      481 ctcgtggaag ccctccatcg agatgaccgc gcctccatcc ggagcgctga aggccaggcg
      541 gaccggcagg ccgcgatccc agtcgatccg ctcggcggcc cagccaaggc agctcgtcat
      601 cggctcgccg tcggcgtcca acagctcgta ggttcggcct tcctcatcga gcctgacgcg
      661 ctgtcggtag tagccctcga aggtgatagc cggagcgccc gcctcgtcga agaacgccat
      721 gtcgacgacc agcgccccgc cccgccgctc cttaaccgag acggacctgt ggaaaccgag
      781 cgacagggtg acaaccggtc ggccgagctc gtcgaagtgc gaccgggtct cctcgcctcc
      841 ctgggcgatg cgccgcgaat cgagcgcgta gccgtaatag ccgctccaga gccggccatc
      901 ggcggtccga tagctcagat cgtagcctcc gttccgaggc tcgatgccga cgatatacgt
      961 attcgagcag tccggcacgc gacggccgtc cgtcgcgtag aaactcaggc tcaggccgtt
     1021 ccgcgtccgg ctctcgcgcc gcgtcgcgta gccggcctcg ttcatcatcg gatagccgta
     1081 cgagctctcc cagaccgacc aggcgcccgt cgcgtcgcgt ccggttcgga tcctggcggc
     1141 ccactggagc aggtcggacg gcctcaggcg gccgtagatg aaatgctcga tcgccaggag
     1201 accgccttcc gaaccatacg aaaagcggta cgcggcaccg agcatggccg cctcgaagtc
     1261 gataggcagc tcccccaccg gttcgtacgg gttgtcatag aggcagagcc tataccagcg
     1321 ttcttctgcg gagccgggga aagccggagg cccggacggt ccgacgggct cgacgtagtc
     1381 gggcaaaggc tcgcccacga caggagcgag cgggttcggc gccctgaacc gcagatcgtt
     1441 caggaagcgc ctcgtctcct ccatcgcgag cggatcgagc ggcgcgtagg ccgaggccat
     1501 cgacgacagc gcctcgacga ggcctgcgag cgcccgccgg acggacgagt cggaaccgta
     1561 gctgccctgg gccgcctcgc gcgcggccca gggcagcgct atgagcgcgg cgcgggcccg
     1621 gccggccggc tcgaggtcct cacgccccgg cgaggcccgt agcgacgcgg tcgagattat
     1681 cgcctcgagc tcgacgagct cgcgcgcggc gcgctccagg tagtcggcga gcccccgtgc
     1741 cgacgcagcc ttggtggcgg cattgacagc gtcgtaggca tagtcgaggt ccaccctgaa
     1801 cagggcggac gccgcgccgc gcaggttatc gtagacggcg ctgccgagca gcaagtcgat
     1861 ataatcgatg gccgtgccct ccggctcggc caggcgcaac acctcgagca ggtagatcga
     1921 ttcgacgtag aggccgctca tcatggcgac cagctcggcg gcagcgttgc gccccgaatg
     1981 gacgccaggc accgccggcg aggcgtcggc atagtagagg gccgcggccg cccgcaggaa
     2041 cgaggagcgg tagacggcgt cgtcctggga caggctagcc agctcgtcgg cgttgacgac
     2101 gatggcccgc ttcgcggcgc cgacgcgggc cggccagaag ccgggcaggg gcggcacgtc
     2161 gtcgaccgag cctatgacga ggcggcccga cgcgacggaa cgctccacgt actcgatcag
     2221 gtagcggaac tcgtcgtagc gccccgcgga cgagaaatcg gccaccaagc gcctgagcgc
     2281 ggcacccgga tcgatcgttg accggagagg cgccttgccc aggcgtacgt actgcgagca
     2341 gccctcccag agtctggcac cggcggcggt cagagcgtcg aggctcgctc catcggcccc
     2401 gaaccccagc attgcggtta cggccgcgaa caaaatgagc gcaatgcaat gccgtactct
     2461 gcgaatcatg ggccctccgg gctatcgata gtaggccggg acgagatcga ccacgaccga
     2521 atccaggaaa ccgagttcca ctacgagcga tcctgccgcg tcgatgggag cgccggcgag
     2581 gtcgggcagg cgataggccg taccgctcag ctcgccgagc gccgtaccga tagggatggt
     2641 gatcctgccg ggcgcggtac cttccgccgt ctcgagcgac agggcgacga cgacgagcct
     2701 ggcgctgtca ccggcgcgta tctgccacca ggccaggccg tcgtcgacgg agcgatcgat
     2761 tatggcggcc gaggccagga acggccgcag gccgacgtag aagcgctcga tggcggcgta
     2821 gccggcggcg aagaccgcgt cgtcggccca gtcgagcccg cgtacggaca gtccggcctc
     2881 gtagagtccg gtcgagccgt cctggaagcc catcgtctcg aacatcggcc gcctgcccgc
     2941 cccgacggac aggaagcggg ccgcgacgtg ccgtagccgc atcctttcgg gccccatgac
     3001 gcgtcgtagg ggcgagcccc agagcctagg ggcggacgac tcgggcaggt cgaccgcgaa
     3061 gcataccgag gttcgcgctc cgccggtcct ctcggccgcc agcgagtcgt acagcgagaa
     3121 cgcggcgcgc aggagcggcg cgtcgacgcg gcgcagcgcc tcgccgccca tcagcaggtc
     3181 gaagccgaga gcgccgaagg ccccagtctc gtcgcccttg ctcggcgaga tggccccgac
     3241 gcctggcgct ccgtccctgg acgcgcggat ggcctgggcc acgacctcgg aggtcgggcg
     3301 gtcgcaggac ggcaccgatc cctcgtcgtc gacgctctcg gagaatacgc gatcggcggc
     3361 gttgtacctg acgaaatcga agccgaagga atcgcgccag taggagaaga cctcggcgta
     3421 gtatccgata gcctcgtcgt tacggcggac cggctcggtc gggatggagt tcggcggcat
     3481 atcgtcatag aacgcgaacg gcgcgacgac gctataggcg tcctctccga tatcggcgcc
     3541 gtcgatgtcg cggtacgaga agatcgggaa gtcgtcggac gagtcgcagc gtaggaacga
     3601 cggaacgccg acgccggccc gcgcgtgggc cggcacgggc cagaggcctc tgtcggcgca
     3661 gagcctgatc gccgagtaat aggccttgac cttggcgtcg acgacctcgg gggcgtcgcc
     3721 ctcgatcaca cggaagctac ggatgccgta atcctcctta accgtcgcca ctatgtcgga
     3781 gacctgctcg gcgtcgcgga gcctgtcctc ggagcggtac gggaacgcgg ggtcggcgtg
     3841 agccaagccc cgccgctcgt cgtcgagcgc gacccagcgg aacagctggg gccgttccat
     3901 cgcgatgcgg ctgaactggg cggcgtacgg cagaagctcg tacccgacgc acttgccgag
     3961 cagatggcaa gccgacacga aggcgcggag ctgattctcc gcgctgaccc ccgcgccgac
     4021 gagctcccgg tcgaacaggg tcgggtcggc catcgtcatc gtctcgggcg cgtagcacag
     4081 gtcgaactgg gtcgggtgaa agggcgctag caggatggcg tcggaggcta tagccggtag
     4141 cagcttggcg gccctgatga aggttccggg caagcttccg gacgcgcagg cgcgtacgtt
     4201 gatccagcag aagtcggact cgagcatcca ggatgagtcg ctacgcagcc tacgcggctc
     4261 gggaggagaa ctcctggcct cgagcgtcgc gtacagcgac gccatggccg cgtcgctcgc
     4321 cgcgatcaat acgctctgcg tgatcgcgag cggcaggccg cggtcgatgg cggccgccaa
     4381 atcggcggcc agcgccgtat cttcgcggga gggcgtcagc gccctctcga tatcgctcca
     4441 tatcggcata cggcactata gcgtccgggg gcgcgccctg tgaagcgagt cgccgcggcg
     4501 cttgccgtcg tcgtcgcggt acggattcct ggcgttctta aggtaggcct tgtacgcggc
     4561 gcaggcctcg tagtagccgt ccggccccct gaaggtcgac agctcgtcgc aacgatacag
     4621 ctcgaggagc gtcgggaaca gcgggctgtc gagcgcccta ccgacgctcg acggggggat
     4681 gcgcggcagg gcggcgggca gcatgtggta gcgtacgagg aaggcgaccg agtcgacaac
     4741 gtcgcccggg tagccgagcc gcccgagcag ccgcctggcg gcccgctcgc ccagctcgga
     4801 atgcccgtcg aaacggcgtc cgccggccgg tacggcatcg ggtttcccgg tatcgtggag
     4861 tagcagggcg agcgacagca cgaggtcggg cgccttgcgg tggccgaagg tctccatcgt
     4921 atgccgccac gcgtctccct cggggtggta atccttggcc tgggacacgc tcccgagctc
     4981 ggcgacctcg ggccagtatt cgtccacgaa gccggagcgc cggagcagct cgaagccctt
     5041 gtcggggcgg gccccggtga cgacaaggtc gagcaggtcg cgctggtact cgacaggcag
     5101 gccggacgga gtctcgggta ggtaggacgg gtcgggttca tagcggtacc gggccgcgag
     5161 cgccgccgcc tcgaacagga tcagctcggg cgacgcctgg cgcggagaag gtgtcggctc
     5221 ggacaggctc tcgtacacgc ccccgcgcgc gtcgaaggag cgtttggcag ggagcctcgt
     5281 aagcgcggtc tggggatagg ctccagcttc gccgtcggaa cagcggtaga gcacgcggct
     5341 ggccgggccg ccgccgacgg cctcggcgta aggcaggccc gggaaggaca ggccatcggt
     5401 tgccttggcc aggccgacga tatcgccatc gaagcgctcg aagcgcggcg gggccgggct
     5461 gacgccgaaa tactcgtcga gcgccgtgaa cgagcaaacc accgaggcct ccaggct
//
LOCUS       DAOPHV010000111         5510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_19540_length_5510_cov_4.853346, whole genome shotgun sequence.
ACCESSION   DAOPHV010000111 DAOPHV010000000
VERSION     DAOPHV010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19540_length_5510_cov_4.853346"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PLQ29_04270"
     CDS             <1..187
                     /locus_tag="PLQ29_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85892.1"
                     /translation="EGGRVTVTVGDDGPGMPEPPPSDASGGGFGLTLVSLLATQLRAD
                     IARESDGGTVVRLSFEL"
     gene            complement(243..1016)
                     /locus_tag="PLQ29_04275"
     CDS             complement(243..1016)
                     /locus_tag="PLQ29_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPG85893.1"
                     /translation="MIHSARPGRPGLVVVGLLFMALTLVGVRAEAQTRVLMVTDTWPP
                     FRMEETSFGTIKDGIDFQIIRRIGDELGLELEIQYHPWARALAMLRSGQADIMTGVAY
                     SPERDEYLRYVPTAYAAVRPVFYTRTGMAASLRSYDDLAGKTIGQSASSVYFEPYDSD
                     ATLGKLSLQYEEQVLRMLALGRIDVAVGTEPNIGWDALRLGLSGQVEPALFRPDASTE
                     LFIVVSERSPLVARSGEIDAILRRMLASGEIDAIMAAYR"
     gene            1201..1710
                     /locus_tag="PLQ29_04280"
     CDS             1201..1710
                     /locus_tag="PLQ29_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HPG85894.1"
                     /translation="MIKKKLCLLGSLGVGKTSLIRKFVLNVYSETYLCTVGVKVDKKI
                     VRLRDGEDVILMIWDMEGQDDFSSLADTYLRGLSGYLCVADGTRPETLRTALSICSSM
                     NSMFQGVSSSLLVNKCDLTDRWAVTEEECHDLAPGCGRILRTSAKLGIGVEEAFVDIA
                     GRMVERPHG"
     gene            1703..3274
                     /locus_tag="PLQ29_04285"
     CDS             1703..3274
                     /locus_tag="PLQ29_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85895.1"
                     /translation="MADRRAGRPIDERLRELDPRERQNLDALAKRMRGARLTPRLVGE
                     TLPEAVLIGDEGSRPLSRSMTGITERAIAASVEDNPEALSTALFPIIGAAIRKALNKM
                     LAEMMESMNSGLERAFSFKRLGWRFESWKTGTPFVEIVLRETLRYRVEHVFLIHSKTG
                     LLLREASPPGVVSPDSDMVASMLEAVREYIKDSLALAKTDAVQSIRTGSYALLVEDGP
                     YASIALFVRGSPDPSLRNAAQEAVETIHLRLGSRLKSFSGDVGPFEEADPALRRCLLS
                     QDTRAPGGKPVYAIVALSAILLAAMTAVGFGIAAARSREAFERALDEEPGIVLVDSRR
                     SRGRRVLRVLRDPGAATVAELAEGMGVDAAAFVVEEEPYWSPELRPGVPVAEDAPLVV
                     PEELLELARRLGGIVILFEQDSGELRTGQQAAIRAAGDLLSELIGKSRAYGLDVSVDI
                     VGHAAGEVQDEAGLRVSEERAQKALAMFAELNAPLVDYVRPVGVGVAEPVAPTDGAED
                     ERALNRSVTFKAIFR"
     gene            complement(3255..4124)
                     /locus_tag="PLQ29_04290"
     CDS             complement(3255..4124)
                     /locus_tag="PLQ29_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85896.1"
                     /translation="MANAIARETRKAEDDPRKRVEAIVRAMALAFDSPLTGLELGARD
                     EYEALLAPDGGYNRATIRRVASVLNAYLALFGQDGVARAGARAASRVCEENDELLRYL
                     ASLEPPDRVAERKAGFVPANLGASFVRKGLGLPLFGRFRPSAPPTAPGLILAYRSAER
                     DGTARLYVTRNGPGAAITYAGLGAELSSAARAMECAIVPADVYEPEAGCDAHDFGRYV
                     VVMARRPSRPRRRPVTTLWDPAEYGFLYYIPDDGLAADRLARFLRDLLSGMFGVPHRE
                     AERLAALFSERSP"
     gene            complement(4129..5232)
                     /locus_tag="PLQ29_04295"
     CDS             complement(4129..5232)
                     /locus_tag="PLQ29_04295"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPG85897.1"
                     /translation="MLVNGTDILEPLGICYESLDGDGRIVPANERFWAMADSPMPSGP
                     VSLDEGFAELVGLERELDALMHEGSGQVRLSGFRRGERYLNLTILPGVAVLQDATGEM
                     LAKLTVVQSKNEMAILDEALHSKNAELLEAYGRLDELMNTIRTHNHDLELEVRKRTSE
                     LLRSRLSVITTLARVAEFRDTDTGRHTFRIGQASVMIGRELGLPEDRCEQLYYASLLH
                     DIGKIGIPDSILLKPGRLDESETRVMQGHTRIGAEILDRKDHSLFDTAKRVALYHHER
                     WDGSGYPEGLAGEAIPLVGRICAVADVFDALLSKRPYKEPWPLDEALGEIAARGGTQF
                     DPTIVAAFMRVADAIASLHDDDETGELLPPELD"
     gene            complement(5321..>5510)
                     /locus_tag="PLQ29_04300"
     CDS             complement(5321..>5510)
                     /locus_tag="PLQ29_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HPG85898.1"
                     /translation="KDDERRRLLERLPAGSRDRVRAAYEALGRARMSDCDAAGFRIVE
                     IIKALEREGRIGIVRQGD"
BASE COUNT          892 a         1798 c         1835 g          985 t
ORIGIN      
        1 cgagggcggc cgggtgacgg tgacggtggg cgacgacggg ccaggtatgc ccgagccgcc
       61 gccgagcgac gcttccggcg gcggtttcgg gttgaccctc gtgtccttac tggctacgca
      121 actccgagcc gatattgctc gcgagtcgga cggcggcacg gtcgtgcgcc tgtctttcga
      181 gctgtaatcg gctacgcccg cgtcctggag gcacgcgctg gatatcgatc cggaggaggc
      241 gtttatcgat aggcggccat gatcgcgtct atctcgccgc tggccagcat gcggcggagg
      301 atcgcatcga tctcgccgga tctcgcgacg aggggcgagc gctccgacac gacgatgaac
      361 aattccgtac tggcatctgg tctgaagagg gccggctcga cctggcccga gagtccgagc
      421 cggagcgcat cccagcctat gttcggctcg gtgcctacgg cgacgtcgat gcggcctagg
      481 gcgagcatgc gcaatacctg ttcctcgtat tgcaggctca acttgccgag ggtagcgtcg
      541 gagtcatacg gctcgaaata tacggagcta gcgctctggc ctatcgtctt accggccagg
      601 tcgtcgtagg accgtagcga cgcggccatg cccgtccgcg tgtagaagac aggccggacc
      661 gcggcatagg ccgtcggcac gtagcgcagg tactcgtcgc gctccggcga gtaggccacg
      721 ccggtcatga tgtccgcctg gccggacctt agcatcgcga gcgcccgcgc ccagggatga
      781 tactggatct cgagctcaag gccgagttca tcgccgatcc gcctgatgat ctggaagtct
      841 atcccgtcct taattgtacc gaagcttgtt tcttccatcc ggaacggcgg ccaagtgtcg
      901 gtgaccatga ggacacgcgt ctgggcctcc gcccgaacgc cgacgagcgt gagcgccatg
      961 aacaggagac cgacgaccac gaggccgggg cggcccggtc gcgctgagtg gatcatgtat
     1021 ctagtctagg tttccccgac accgtagtca aatgtaggtt agtaggcggc tttggggcgc
     1081 ctacccggtt aggaccggga tgcggatcag ggtttggcga tgacggcagc ctccttggga
     1141 tagcttcctt tacgcttcgc gtattctagg cgatactggt acccggagag cagaaaacgg
     1201 atgataaaga agaagctgtg ccttctaggt tcgctcggcg tcggcaagac gagcctgata
     1261 cgaaaattcg tcctgaacgt gtattccgaa acctacctgt gcactgtcgg cgtcaaggtc
     1321 gacaagaaga tcgtgcgcct gcgcgacggc gaggacgtga tcctgatgat ctgggacatg
     1381 gaagggcagg acgatttcag ctctctggcc gacacctacc tacgcggcct gtccggctat
     1441 ctgtgcgtgg ccgacggcac gcggccggag acgctcagga cggccctgtc catctgttcg
     1501 tcgatgaact cgatgttcca aggcgtcagc tcatcgctac tcgtcaacaa gtgcgacctg
     1561 acggatcgct gggcggtgac cgaggaggag tgccatgacc tggctcccgg ctgcggccgg
     1621 atcctaagga cgagcgccaa gctcggtatc ggcgtcgagg aagccttcgt cgacatcgcc
     1681 ggccgcatgg tcgagcgccc gcatggctga tcgccgggcc ggtcggccca tcgacgagcg
     1741 cctgagggag ctggacccgc gggagcgcca gaacctggac gcgctggcaa agcggatgcg
     1801 tggcgccagg ctgacgccgc gcctggtagg cgagacgctg cctgaagccg tgctcatagg
     1861 cgacgagggc tcgcggcccc tgtccaggtc gatgacggga ataaccgaac gggccatagc
     1921 cgcgtccgtc gaggataatc ccgaggcgct ctcgactgcc ttgttcccga taatcggcgc
     1981 cgccatacgc aaggccctga acaagatgct ggccgagatg atggagtcga tgaacagcgg
     2041 gctcgagcga gccttctcgt tcaagcgcct gggctggcga ttcgagtcgt ggaagaccgg
     2101 aacgcccttc gtcgaaatcg tgctcaggga gacgctacgc taccgcgtcg agcacgtgtt
     2161 cctgatccat tcgaagaccg gactccttct tcgcgaggcg tcgcctcccg gcgtcgtatc
     2221 gcccgacagc gacatggtcg cctcgatgct cgaggcggtc agggagtata tcaaggactc
     2281 gctcgcgctg gccaagacgg acgccgtcca gtccatcagg actggctcct atgctctgtt
     2341 agtcgaggat ggtccctacg cgtccatagc cttgttcgtc cgaggctcgc ccgacccgtc
     2401 gctccggaac gccgcccagg aggccgtcga gacgatccac ctgcggctcg gctcgcggct
     2461 caagagcttc tcgggcgacg tcggcccctt cgaggaagcc gatccagccc tgcgccgctg
     2521 cctgcttagc caggataccc gggcgccggg tggcaagccc gtctacgcca tcgtcgccct
     2581 gagcgcgatc ctcctggcgg ccatgaccgc ggtcggcttc ggcatagcgg cggcgaggtc
     2641 ccgggaggcg ttcgagcgcg cgctcgacga ggagcccggc atcgtcctgg tcgactcgcg
     2701 gcgatcgcgc ggccggcgcg tgctacgggt gctccgcgac cccggggccg cgaccgtagc
     2761 cgaactggcc gagggcatgg gcgtcgacgc tgctgcgttc gtcgtcgagg aggagccgta
     2821 ctggtcgccc gaactcaggc ccggcgtccc ggtagccgaa gacgccccgc tcgtcgtgcc
     2881 ggaggagctt ctcgagctgg ccaggcggct tggcggcatc gtcatcctgt tcgagcagga
     2941 ctcgggtgag ctacggacgg ggcagcaggc ggccatccgc gcggccggcg acctcttgtc
     3001 cgagcttata ggcaagtcgc gcgcatacgg cctggacgtg tcggtggata tagtcgggca
     3061 cgccgccggc gaggttcagg acgaggccgg tctccgcgtc agcgaggagc gggcgcagaa
     3121 ggccctggcc atgttcgccg agctgaacgc cccgctcgta gactacgtgc ggccggtcgg
     3181 cgtcggcgtc gcggagcccg tggcgccgac tgatggtgcc gaggacgaac gcgccctgaa
     3241 ccgtagcgtc accttcaagg cgatctttcg ctgaacaggg ccgctagcct ttccgcctcg
     3301 cggtgcggta ccccgaacat gccgctgagc agatcccgta ggaaacgggc aagcctgtcg
     3361 gccgcgagtc cgtcgtcggg tatgtagtat aggaagccgt attcggccgg atcccagaga
     3421 gtcgttaccg gtcgccgtct gggccgagac gggcggcggg ccatgacgac gacgtaacgg
     3481 ccgaagtcgt gggcatcgca ccccgcttcc ggctcgtata cgtcggccgg tacgatggcg
     3541 cattccatcg cgcgcgccgc gctcgacagc tcggctccca atccagcata cgtgatcgcg
     3601 gcacccggtc cgttgcgcgt cacgtatagg cgagccgtgc catcccgctc ggccgaacga
     3661 tacgccagga tcagccccgg ggccgtgggc ggcgcgctcg gccggaatcg gccgaagagc
     3721 ggtaggccca agcccttccg aacgaaagac gctcccagat tggccggtac gaagcccgct
     3781 ttgcgctcgg cgacccggtc cggcggttcg agcgatgcca ggtagcgcag tagctcgtcg
     3841 ttctcctcgc atacccggct cgcggccctg gctccggccc tggcgacgcc gtcctggccg
     3901 aataacgcga gataggcgtt gagtaccgac gcgactctgc gtatcgtggc gcggttgtag
     3961 ccgccgtccg gggcgagcaa ggcctcgtat tcgtctcgag cgccgagttc gaggccggtg
     4021 agcgggctgt cgaaggccag ggccatggcg cgtacgatcg cctcgacgcg ttttctagga
     4081 tcgtcctcag ccttgcgcgt ctcacgggcg atggcgttcg ccatggcgtc aatccagttc
     4141 cggcggcaat agctcgcccg tttcgtcgtc gtcatggagg ctcgcgatgg cgtcggctac
     4201 gcgcatgaac gccgcgacga tggtcgggtc aaactgcgta ccgcctcgcg ccgctatctc
     4261 cccgagcgcc tcgtcgagcg gccacggctc cttgtacggg cgcttggaca ggagcgcgtc
     4321 gaagacgtcg gccacagcgc agatacgccc gacgagcggt atggcctcgc cggccagccc
     4381 ctccggatag cccgaaccgt cccaccgctc gtggtgatag agcgcgactc gtttggccgt
     4441 gtcgaagagg ctgtgatcct tacgatccag tatctccgcg cctatgcgcg tatggccctg
     4501 catgacgcgc gtctcgctct cgtccagccg gcccggcttg agcaggatgg agtccggtat
     4561 gcctatcttg cctatgtcgt ggagcaggct cgcgtagtag agctgctcgc agcggtcttc
     4621 cggcagcccg agctcccggc caatcatgac gctggcctgg cctatcctga aggtgtgcct
     4681 gcccgtatcg gtgtccctga attccgccac gcgggcgagc gtcgttatga ccgacagcct
     4741 cgagcgcaac agctccgagg ttcgcttcct gacctccagc tccagatcgt ggttgtgggt
     4801 cctgatcgtg ttcatcagct cgtccagccg accgtaggcc tcgagcagct cggcgttttt
     4861 cgaatgcagg gcctcgtcga gtatggccat ctcgttcttg gactgaacga cggtgagctt
     4921 ggcgagcatt tcgcccgtcg cgtcctgcaa cacggccaca ccgggtagta tggtcaggtt
     4981 caggtagcgc tcgccgcgcc tgaagccgct cagcctgact tgtccgctcc cctcatgcat
     5041 cagggcgtcc agttcgcgct cgagtccgac cagttcggcg aagccctcgt ccaggctgac
     5101 tggcccggag ggcatgggcg aatcggccat ggcccagaag cgctcgttgg ctggaacgat
     5161 ccggccatca ccgtcgaggc tctcgtagca aattcccaaa ggctccagta tgtctgttcc
     5221 attgacgagc atactttgat tgtaggccgt catgccccta cggtcaacgc gaggcggatc
     5281 ggaccggtac gaccggaccc gggccgcctc gtgaaccgcg ctagtctccc tgcctgacta
     5341 tgccgatgcg tccttcccgc tccagcgcct tgatgatctc cacgatcctg aagccggcgg
     5401 cgtcgcagtc ggacatgcgc gcgcggccga gcgcctcgta ggccgccctg acgcggtcgc
     5461 ggctcccggc cggcaggcgc tcgagaaggc ggcgccgctc gtcgtccttc
//
LOCUS       DAOPHV010000112         5476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_19703_length_5476_cov_3.531267, whole genome shotgun sequence.
ACCESSION   DAOPHV010000112 DAOPHV010000000
VERSION     DAOPHV010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19703_length_5476_cov_3.531267"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..451
                     /locus_tag="PLQ29_04305"
     CDS             11..451
                     /locus_tag="PLQ29_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85899.1"
                     /translation="MKRFLIILIAVSIAAGAFAQGATGSSGDSGRDKVWNAVMESDLY
                     PVRVDVSRVYSHAQGYRVVYRKGATSFAEVYIPVEWFVAGGKGMLLRGRGPQYPYMVV
                     YYRPDGGFSHVKLFVLANMRDASWGVIEGDPGDRFKVDSIKLEF"
     gene            559..1542
                     /locus_tag="PLQ29_04310"
     CDS             559..1542
                     /locus_tag="PLQ29_04310"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPG85900.1"
                     /translation="MTQEKLIQGSGILRFIDTHGRFILVGHKEPDGDCVGSQLALASF
                     LERIGKRVYLLSSGPFTRTEISGYASRFSDKADPSMAGEGSAIIVLDCSSLSRIGDIR
                     LSLPEGLPLAFIDHHASGNASGDAVLVDPRAPAVTYLVQSIIEAHSGRPTKEEAELLL
                     FGLCTDTGFFRHLDETSGDVFASVGRLVEAGASPKRAFDMMNGGKPFASRKLMGELLS
                     KAETRYDGKLIVTSMSLADQERYGLVGRDSDMLYQLLLTVAGMEVAVVVRQESPTDCT
                     VGFRSREAVDVASVAASFGGGGHRLAAGLHITGQAADIVERVIEAFKPLLA"
     gene            1742..2605
                     /locus_tag="PLQ29_04315"
     CDS             1742..2605
                     /locus_tag="PLQ29_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85901.1"
                     /translation="MSNEELNDLVDRKKHGELGLERRILELMATDVYKRPWVYGLKSE
                     DEVGEVFVRYWTRIGNLANRYEDTGYRFEAFAISSFRYMALSLKRQRVCRYDRDGAVI
                     DDEVFEAWEDLRRCEYSAPDDDIPGSTGPAFPCRDDVGVVATSFRRRIVFLCVKCAHL
                     LDDGQVAAIAEEMGFDLAELLGLLERARNAYPVINPRIASKRRGRNVAWIRMGAAARR
                     LAREADEDTRRYLRGTVERERGLYLRAARRIAHMNRLIPNKAVAELLGIPKGTVDCGV
                     GRVLRMYSGYS"
     gene            2643..3242
                     /gene="rdgB"
                     /locus_tag="PLQ29_04320"
     CDS             2643..3242
                     /gene="rdgB"
                     /locus_tag="PLQ29_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPG85902.1"
                     /translation="MTIYIATGNAHKLDEIRPALPGVDVRLPSDAGVDFDYEETADDF
                     IGNSVGKALTLWRAVGAPVLADDSGLCVDALGGRPGVLSARYGSPDGVTPLGAAERNA
                     RLLAEMRGRSDRSCRFVCCMTLVLSRDRFFVIQEACEGVLLEEPRGSGGFGYDPLVLL
                     PELGRSVAELSVEEKNRVSHRGRALVRVSSLLGSLVAAP"
     gene            complement(3339..3542)
                     /gene="rpmE"
                     /locus_tag="PLQ29_04325"
     CDS             complement(3339..3542)
                     /gene="rpmE"
                     /locus_tag="PLQ29_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPG85903.1"
                     /translation="MKSGIHPKYEFTTITCACGNVIETRSTVKNIQVEICSACHPFFT
                     GKQKLVDTAGRIDRFRRKYGIKE"
     gene            complement(3618..5318)
                     /gene="rho"
                     /locus_tag="PLQ29_04330"
     CDS             complement(3618..5318)
                     /gene="rho"
                     /locus_tag="PLQ29_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPG85904.1"
                     /translation="MAILRKSQSDETSSGAPEAKPQGELGLEISTESLEQSDRDDGTA
                     KPAPRKRIVRRPVVKQDEQSPAQPQVQVATEPEAATTQGGYPPSNGAHAGFSHSQRHE
                     ADEGRRFEGRGGLVPGFVRKQESFEKAKEDAPKQEQGEARSKLSINELTSMGFKELRE
                     LGGGTGLNHEEMMVLKKQELIFQILKAHTERGGIIYAYGSLEILPDGYGFLRSPQNSY
                     LPASDDIYISPSQIRLFNLKTGDTVFGQIRSPKEGERFFAMLRVEQVNFDEPAVAQNR
                     IPFENLTPLYPNQRLNLETTTAETSTRIINLFCPIGKGQRALIVSPPKTGKTILLQKI
                     ANAITANHPEVYLIVLLIDERPEEVTDMERTVKAEVISSTFDEQATRHVQVSEMVLDK
                     AKRLVEHGKDVVILLDSITRLARAYNQTVPTSGKILSGGVDSNALHKPKRFFGAARNI
                     EGGGSLTIIATALVDTGSRMDEVIFEEFKGTGNMEINLDRRMSDRRLFPAINVKKSST
                     RKEELLLASDELQKIWILRKALSPMDDLEATELLIDKMSKTKNNEAFLRSMNTPLGDS
                     "
BASE COUNT          952 a         1628 c         1738 g         1158 t
ORIGIN      
        1 tggaggaagc atgaaacgat tcttgattat cctcatcgct gtatccatag cggctggagc
       61 cttcgcgcag ggtgctaccg gctcctccgg cgattcgggc cgcgacaaag tatggaatgc
      121 cgtgatggag tccgatctct atccggtacg cgtcgacgtc tcgagggtat attcccacgc
      181 tcagggctat agggtcgtct ataggaaggg ggctacctcg ttcgccgagg tatacatacc
      241 ggtcgaatgg ttcgttgcag gtggcaaggg catgctactg cgtgggcgcg ggccccagta
      301 tccgtatatg gtcgtctact atcgccccga cggcggtttc agccatgtca agctattcgt
      361 actggccaac atgcgcgacg cgagctgggg cgtcatagaa ggcgacccgg gagaccggtt
      421 caaggtcgat tcgatcaagc tcgagttcta aggcggccta gcccgaccat cagctcttcg
      481 atatactagg gcgaccccaa gccggtggcc ggggtcgccc attcgcgcgg caggcaactc
      541 aactatccag gatgtatcgt gacgcaggaa aagctcattc agggatcagg tattcttcgg
      601 ttcatcgata cgcacggccg cttcatactg gtcggccaca aggaaccgga cggcgactgc
      661 gtcggtagcc agctggcgct cgcttcgttc ctcgagcgga tcggcaagcg cgtctatctc
      721 ctatcgtccg gcccgttcac caggaccgag atatcgggct acgcgtcccg cttctcggac
      781 aaggccgatc cctccatggc gggcgagggg agcgcgatca tcgtcctcga ctgctcgtcc
      841 ttatccagga taggcgacat ccggctgagc ctgcccgagg gactgcctct ggccttcata
      901 gaccatcatg cctccggcaa cgcgtcgggc gatgcggtgc tcgtcgaccc gagggctccc
      961 gccgttacct atctcgtgca gtccatcatc gaggcccatt cggggcggcc gaccaaggag
     1021 gaagccgagc tgctactctt tggcctatgt accgataccg ggttcttcag gcacctggac
     1081 gagacttccg gcgacgtctt cgcatccgtc ggccgcctcg tcgaggcagg cgcctcgccg
     1141 aagcgagcct tcgacatgat gaacggcggc aagcccttcg cctccaggaa gctgatgggc
     1201 gagctcctgt cgaaggcaga gacgcgctac gacggtaagc tgatcgttac cagcatgagc
     1261 ctggctgacc aggaacgcta cggtctcgtc ggccgcgact ccgacatgct gtaccagttg
     1321 ctgctcaccg tcgccggtat ggaagtggcc gtagtcgtca ggcaggagag cccgacagat
     1381 tgcaccgtcg gtttcaggtc gagggaggcc gtcgatgtcg cttcggtcgc agcgtcgttt
     1441 ggcggaggcg ggcatcgcct agccgccggg ctccacataa ccggccaggc cgccgatata
     1501 gtcgagcggg ttatcgaagc cttcaaaccg ctgctggcgt aggcgaatgt aactacttgt
     1561 aacagccgaa tcgatgccgt cgggatttag catctcggcg gcctttactt ttggctcgta
     1621 agtcgatgct gaggcattac ttggatctcg atcaagggat agccacctcc atgtaggtac
     1681 gcttcttgca ataactgatt tcagctattg tcggcgttct tcacagaaac gaggtgcgtc
     1741 tatgtctaat gaggagctga acgacctggt cgatcggaag aagcacggcg agttgggctt
     1801 ggaacggcgc atcctggagc tgatggctac ggatgtctat aagcggcctt gggtctacgg
     1861 actcaagtcg gaagacgagg tcggcgaggt gttcgtacgc tactggacca ggataggcaa
     1921 cctggccaat cgctacgagg ataccggcta tcgcttcgag gccttcgcga tcagctcgtt
     1981 ccggtacatg gccctttcgc tcaagaggca gcgcgtctgc cgctatgata gggacggcgc
     2041 cgtcatcgac gacgaggtct tcgaagcctg ggaagaccta cggcgttgcg agtactcagc
     2101 ccccgacgac gatatccctg gcagtaccgg accggcgttc ccctgcaggg acgatgtagg
     2161 ggtagtcgcc acttctttca ggcgacgtat cgtgttcctt tgcgtcaagt gcgcgcatct
     2221 cctcgacgac ggccaggtcg ccgcgatagc cgaagagatg ggcttcgacc tcgccgagct
     2281 gctcggcctc ctcgaacggg cgcggaacgc ttaccccgtg ataaacccgc gcatcgcctc
     2341 gaagcgtcgg ggcaggaacg tggcgtggat caggatggga gccgcggcgc gtaggctcgc
     2401 gcgcgaggcg gacgaggaca ccaggcgata tctacgcggc accgtggaac gggagcgtgg
     2461 actgtacctc cgggccgctc ggcggatcgc ccatatgaac aggctcatac ctaacaaggc
     2521 cgtcgcggaa ctgctcggca tacccaaggg caccgtcgac tgcggcgtcg gtcgcgtgct
     2581 caggatgtac tcgggctatt cgtagacctg ttcggcgggc tagaatgccg gtcggaggaa
     2641 cgatgaccat ctacatagcg accggcaacg cccataagct cgacgagata cgcccggccc
     2701 tgccgggcgt cgacgtacga ctaccatcgg acgcgggcgt cgacttcgac tacgaggaga
     2761 cagccgacga tttcatcggc aattccgtgg gcaaggccct gaccctctgg cgcgcggtcg
     2821 gggccccggt cctcgccgac gattcgggcc tctgcgtcga cgcgctcggc ggcaggccgg
     2881 gagtgctgtc ggcgcgctat ggctcccctg acggcgtaac gccgctcggc gccgccgagc
     2941 gcaacgcgcg gttactcgca gagatgcgcg gacggagcga ccgctcctgc cgtttcgtat
     3001 gctgcatgac gctggtcctg tctagggata ggttcttcgt gattcaggag gcctgcgagg
     3061 gcgtcctgct cgaagaacca cggggatccg gcggtttcgg ctatgacccg ttggtactgc
     3121 tacccgaact cggccgtagc gtcgcggagc tgtcggtcga ggaaaagaac cgagtgtcgc
     3181 atcgcggaag ggccctcgtc cgggtctcgt cgctactggg tagcctggtc gcggctccat
     3241 aatccggcga ccgactcgcg gccccaaaaa aaaggccggc agctctaccg agcgccggcc
     3301 ttcttcgtta cgctcccgcg ggcgcaagcc ccggggcgtt actccttgat accgtacttc
     3361 ctgcggaagc ggtcgatgcg tcctgcagtg tctacgagct tctgctttcc ggtaaagaag
     3421 gggtggcagg cggagcaaat ttccacctgg atattcttta ccgtcgaacg ggtctcaatg
     3481 acattgccgc acgcgcacgt gatcgtggtg aactcgtact tgggatgtat cccgctcttc
     3541 atcgtgatct cctcgcgtgc cgctcataca tgccagaata ccggcttgat gacggctaaa
     3601 agtcaggtcc cgccgactca ggaatcgcct aagggcgtat tcatggatcg taggaatgcc
     3661 tcattattct tagttttgga catcttgtca atcagcagct cggtagcctc gaggtcgtcc
     3721 atcgggctca gggccttgcg tagtatccat atcttctgga gctcgtccga ggcgaggagt
     3781 agctcctcct tgcgggtgct cgacttcttg acgttgatcg ctggaaagag cctacggtcc
     3841 gacatccgcc tgtccaggtt gatctccata ttgccggtgc ccttaaattc ctcgaagatc
     3901 acctcgtcca tcctggaacc cgtgtcgacg agggccgtcg cgatgatggt caacgagccg
     3961 ccgccctcta tgttgcgcgc ggcgccgaag aatcgcttgg gcttgtggag ggcgttcgag
     4021 tcgacgccgc ctgacagtat cttgccggag gtcggcacgg tctggttata ggcccgcgca
     4081 agcctcgtga tcgagtcgag caggatcacg acgtccttgc cgtgttcgac gaggcgcttg
     4141 gccttgtcca agaccatctc ggagacctgt acgtggcggg tggcctgctc gtcgaaggtc
     4201 gacgatatga cctccgcctt gacggtgcgt tccatgtcgg tcacctcctc cggccgctcg
     4261 tcgatgagca gcacgatcag gtagacttcg gggtggttgg ccgtgatggc attggctatc
     4321 ttctgtagaa gtatcgtctt accggtcttc ggcggggaga cgatcagcgc tcgctgtccc
     4381 ttgccgatcg gacagaacag gttgatgatg cgcgtggagg tctcggccgt ggtcgtctcg
     4441 aggttgagac gctggtttgg atagagcgga gtcaggttct cgaacggtat gcgattctgg
     4501 gcgacggccg gctcgtcgaa gttgacctgc tcgacgcgga gcatggcgaa gaatcgctcg
     4561 ccttccttgg ggctacggat ctgcccgaag acggtatcgc cggtcttcag gttgaagagc
     4621 ctgatctggg acgggctgat gtagatatcg tccgaggcgg gcaggtagct gttctgggga
     4681 gaacgcagga agccgtaacc gtccggcagt atctcgagcg agccataggc gtagatgatg
     4741 ccgccgcgct cggtatgggc cttcagaatt tggaagatga gttcctgttt tttcaggacc
     4801 atcatctcct cgtggttcaa gccagtaccg ccaccgagtt cgcgtagctc cttgaagccc
     4861 atgctcgtta gctcgttgat ggacagcttg gatcgggcct cgccttgctc ctgcttcggc
     4921 gcgtcctcct tggctttttc gaagctctcc tgcttgcgta cgaagccggg cactaagccg
     4981 cctcggcctt cgaaacggcg gccttcgtca gcctcgtggc gctggctgtg cgagaagccc
     5041 gcgtgggctc cattggacgg cggatagccg ccttgcgtgg tcgcggcttc cggctccgtc
     5101 gcaacttgga cctggggttg agcgggcgac tgctcgtctt gcttgacgac ggggcggcgt
     5161 acgatgcgct tgcgcggagc cggcttggcc gtcccgtcgt cgcgatccga ctgctctagg
     5221 ctttctgtgc tgatctccaa accaagttct ccttggggct tcgcttcggg cgctccggac
     5281 gaagtctcgt ccgactggct cttgcgtaga attgccatca gttactccaa taggttgtgt
     5341 gagccgaccg ggatcgtcgc gggcgccata cgcccgagac cgcgcgtttc cgtgcggttc
     5401 ggttggctcg ggaggcgtgc ttccgagcgt ccctgattct gtctatgctt tttccgtttc
     5461 cggtcggaag atagcg
//
LOCUS       DAOPHV010000113         5457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_19795_length_5457_cov_3.932432, whole genome shotgun sequence.
ACCESSION   DAOPHV010000113 DAOPHV010000000
VERSION     DAOPHV010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19795_length_5457_cov_3.932432"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PLQ29_04335"
     CDS             complement(<1..617)
                     /locus_tag="PLQ29_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85905.1"
                     /translation="MDKDFGSRDDKIDAIITSSGYPLAVKSLLQRLDESDVIASVSLG
                     SGRDAVLLAAVAARLQAEPSLRALVIAPSDREVAGLERTFEGVAGPGGPSSAAIGREA
                     SMEDARASIGSLDAIASRIAAGTLDPDDFALIALSDLDPFAGAASVAMLRHALGSGSM
                     ERRLIGFAEELRPAHRAVVAPLCDSLVEARCLLMASPRPVPPKRRV"
     gene            767..1567
                     /locus_tag="PLQ29_04340"
     CDS             767..1567
                     /locus_tag="PLQ29_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPG85906.1"
                     /translation="MIRFRRMIALIPIVLMAACGSDGAEAEAKAPAVDPRLRIVEEAL
                     GSALPDQAATVFAAVSASPNRFFELLDAAVAESAAAGRLLVLVDKRRPLDPGYEPDDL
                     VSLNDYPLAVTRRDLRLRKAIMGAVLEMDAAARADGVRLVFASSYRSYEYQDGLFKRY
                     VASYGEAEASRFSARPGTSQHQLGAAIDFDPIDEAFASTDAGRWMEANAWRFGFSLSF
                     PRGMESVTGYIWESWHFRYVTKAGATLEREYFGGVQQYMLEFLDAYSR"
     gene            complement(1564..3090)
                     /locus_tag="PLQ29_04345"
     CDS             complement(1564..3090)
                     /locus_tag="PLQ29_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPG85907.1"
                     /translation="MAGNELVVDVNKIKKAAHSAIPLTITTYTLPHEIEVYIEEVLAV
                     FLGELGQARIKDYLVYCLRELAVNAKKANTKRVYFETKGLDLRNPAEYEEGMRTFKAD
                     TMENIAYYLQKQKERQYYIKIIFQARGSNVVLEVRNNVELTRTEYLRIQDKLAHSRKY
                     ETLEEAMSQVLDASEGAGLGLVILVLMLKKMSLDEECFDIHTDNGETVARITVPVAAA
                     KIESLSTLTDELVRQITRLPHFPENILTIQRLISDPKSEMVDIARNISTDPSMTADLL
                     KLVNSAQFMLPRRVDNIVDAVKLVGLRGIKNLLYSYGSQKILADERGMDQRALWDHSY
                     RCAYYSYNLAKNFKPGNRALQDDAYVGGMLHDMGKIIFSAVHPELLDHIKAFCDAKGI
                     DKATFEDLAGGMNHSEIGARVAEKWNFPESLVAAIRFHHEPSLMTTDFKDVVYTVYLA
                     NVFCEYENGGIAYDQIDRDVLQDFGFLSDKQFGKTVEQFSEGFRQESIKQREAAATKR
                     "
     gene            3280..4494
                     /locus_tag="PLQ29_04350"
     CDS             3280..4494
                     /locus_tag="PLQ29_04350"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPG85908.1"
                     /translation="MKEIVIIRGDDADRRFLQVLLSGRYAVRAFGTVAEAAGAALPSG
                     PRAIVVGSEDCSGGGTVDPIAACARVDAGAGVVCVSSSGGPEGASVPPGGEYRVLGRP
                     LLAEALYAAVRLAVAESTLGVEARRRTAAGQAAAEAATAARIEAAPLSPFGRKLLGEG
                     PAMCKVRERIELYAGNEAPVLIVGESGTGKELAAEAIHLGSRRSARRFLPIDCATLSE
                     SLAESTLFGSVKGAFTDAVDKRGAFESARGGTVFLDEIGELALTVQAKFLRTLESGSG
                     SRVGSVDQRRYDVRIVSATNASSLGDARRFRPELFHRISTLVIEMPALRAHKEDLPLL
                     VGALLAEYASEKHVTADTMAKLWGWDWPGNVRELRNVVRRAALLSGGRETILPADIEF
                     DCGARPWQGCLF"
     gene            complement(4517..4978)
                     /locus_tag="PLQ29_04355"
     CDS             complement(4517..4978)
                     /locus_tag="PLQ29_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85909.1"
                     /translation="MKRMMLATAIAALALTVAAAQSAPAGEPSYVGRIELGVSIAGLS
                     ALAETGDEAALAALSDRAILLLGTLSAPSVAVDEPFEAVVEFMQGEWRDPSTLLLHRV
                     FIVFRGEVFRSYLQGSAGKRAVVVARKPTLGPSPHGGYAVYLEVVSARTAS"
     gene            complement(5026..>5457)
                     /locus_tag="PLQ29_04360"
     CDS             complement(5026..>5457)
                     /locus_tag="PLQ29_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HPG85910.1"
                     /translation="SSAAIVFGNERDGLSDAELSLCDEAVRIDTSDLFPSLNLSHAVQ
                     IACWELREAVLATSSREGPGTDRPGRTAAPRSSAARAAARMADAMQAAGLYKLAGRPD
                     AEAFLREVAARAALSETELERLSSLFIRLGALGSLDRGPGR"
BASE COUNT          895 a         1706 c         1831 g         1025 t
ORIGIN      
        1 acccgccgct ttggcggcac cggcctcggg ctggccatca gcaagcacct ggcctcgacg
       61 agcgagtcgc agagcggggc gacgaccgcc cgatgcgccg gcctgagctc ctccgcgaag
      121 ccgatgaggc gccgctccat cgagcccgag ccgagcgcat gcctgagcat cgccacgctc
      181 gcggcgccgg cgaacggatc gaggtcgctc agggctatca gcgcgaagtc gtcgggatcg
      241 agggtgccgg cggctatcct cgaggctatg gcgtcgagcg agccgatcga ggccctcgcg
      301 tcctccatag aggcttcgcg gcctatcgcc gcggaggaag ggccgccagg cccggcgacg
      361 ccttcgaagg tccgctcgag gccggcgacc tcgcggtcgg acggggcaat gaccagcgcc
      421 ctgaggctcg gctcggcttg gagcctggcg gctacggcgg cgagtaacac ggcgtctcgc
      481 cccgagccga ggctgacgct ggcgatgacg tcgctctcat cgagcctctg gagcaggctc
      541 ttgaccgcca gcggatatcc cgacgacgtg atgatcgcgt cgatcttatc gtctcttgaa
      601 ccgaagtctt tatccatgat tatcccgtgg ctattcgttt ctcagcgcgc ctgatacggc
      661 gcacgtatac tgattataca atacaatgcc ataaaaacat ctggaaggct aggcgatcgc
      721 cgctctcgag ttgcccgcga gggcgattat cgtatagagt cgagcgatga tacggttcag
      781 gcgcatgata gcccttattc ccatagtctt gatggcggcg tgcggaagcg acggcgccga
      841 ggccgaagcg aaggccccgg ccgtcgaccc tcggctccgg atcgttgagg aagcgctcgg
      901 atcggccctg cccgatcagg cggcgacggt attcgccgcc gtatcggcgt cgccgaaccg
      961 tttcttcgag ctgctcgacg ccgccgtagc cgagtccgcg gcggccggaa ggctactcgt
     1021 actcgtcgac aagcgccgac cgctcgaccc cggctacgaa ccggatgacc tggtatcgct
     1081 caacgactat ccgctcgccg tgacgaggag ggacctgcgg ctcaggaagg ccattatggg
     1141 cgcagtactg gagatggacg cggccgcgag ggccgacggc gtcaggctgg ttttcgcctc
     1201 gagctaccgc tcgtacgagt accaggacgg gctgttcaag cgttacgtcg cctcgtatgg
     1261 cgaggccgag gcatcgaggt tctccgccag gcccggaacg tcccagcacc agctcggggc
     1321 cgctatcgac ttcgatccga tagacgaggc cttcgcgtct accgacgccg gccgatggat
     1381 ggaggccaac gcctggcgct tcggcttctc gctatccttt ccccggggaa tggagtccgt
     1441 cacgggctat atctgggaaa gctggcattt ccgctacgtg acgaaggcgg gcgcgacgct
     1501 ggaacgcgag tacttcggcg gcgtccagca gtatatgctc gaattcctgg acgcctatag
     1561 ccgttagcgc ttggtcgccg ccgcctctcg ttgcttgatc gactcctgcc tgaagccctc
     1621 ggagaactgc tcgacggtct tgccgaactg cttgtcgctc aggaagccga agtcctggag
     1681 cacgtcccgg tctatctggt cgtaggcaat gccgccgttc tcgtactcgc agaacacgtt
     1741 cgcgaggtag acggtgtata ccacgtcctt gaagtcggtc gtcatcaggg acggctcatg
     1801 gtggaagcgt atggccgcga ccagcgactc ggggaagttc cacttctcgg ctacccgcgc
     1861 gcctatctcc gaatggttca tgccgccagc cagatcttcg aaggtggcct tgtctatacc
     1921 cttggcgtcg cagaaggctt tgatgtggtc gagcagctcg gggtgaaccg ccgagaatat
     1981 gatcttgccc atgtcatgca gcatgccgcc gacgtaggca tcgtcctgga gggcccggtt
     2041 gcccggcttg aagttcttgg ccaggttgta cgagtagtag gcgcagcgat agctgtggtc
     2101 ccagagcgcc cgttggtcca tgccccgttc gtcggccagg atcttctgcg agccgtagga
     2161 gtagagcagg ttcttgatgc ctcgcaggcc cacgagcttg accgcgtcga cgatgttgtc
     2221 gaccctccgc ggcagcatga actgggccga gttcaccagc ttcaggaggt cggcagtcat
     2281 cgatgggtcg gtggagatgt tgcgcgctat gtcgaccatc tcgctcttgg ggtcggagat
     2341 caggcgctgg atggtcagga tgttctcggg gaaatgcggt agcctcgtga tctgcctgac
     2401 cagctcgtcg gtcagagtgg ataggctctc gatcttggcg gccgccaccg gcaccgtgat
     2461 gcgagcgacg gtctctccgt tgtccgtgtg gatatcgaag cattcctcat cgaggctcat
     2521 cttcttgagc atcagcacca ggatgacgag gccgaggccc gcgccctccg aggcgtccag
     2581 tacctgagac atggcctcct cgagggtctc gtacttcctc gagtgcgcca gcttgtcctg
     2641 gatgcgcagg tactcggtcc gggtcagttc gacgttgttg cgtacttcga ggaccacgtt
     2701 cgagccgcgg gcctggaaga ttatcttgat gtagtactgc cgctccttct gcttctggag
     2761 gtagtaggcg atgttctcca tcgtgtcggc cttgaaggtg cgcattccct cctcgtactc
     2821 ggccgggttc ctgaggtcca agcccttcgt ctcgaagtag acgcgcttcg tgttggcttt
     2881 cttggcgttg acggccagct cgcgcaggca gtagaccagg taatccttga tcctggcttg
     2941 gcccagctca ccgaggaaca ccgcgaggac ttcctcgatg tagacctcta tctcgtgggg
     3001 gagcgtatac gtggttatcg taagcggaat cgcgctatgc gccgccttct tgatcttgtt
     3061 gacgtctacg accagttcgt tgcctgccat gcttctctcc gttttcagaa taggacgacg
     3121 cgcctattag attactttat cccggctagt cgtgcaaccg ggataatcga aggcgcggcc
     3181 tgggtggtgc ctcttatgcc cttccgggag tatcgtgtaa tcaggtatcg aaaccttatg
     3241 gatcattgac agcctctcgg cgcggctaga cttagcgata tgaaggaaat agtaataatt
     3301 aggggagatg acgccgatcg gaggttcctg caggttctat tgagcggtcg ctacgccgtt
     3361 cgggctttcg gtactgtcgc cgaggccgcc ggtgccgcgc tgccttccgg gccgcgagcg
     3421 atcgtggtcg gctcggagga ctgttccggc ggtggaaccg tcgatccgat cgcggcctgc
     3481 gccagagtcg acgcgggggc tggcgtcgtt tgcgtgtcct cgtccggcgg gcccgaggga
     3541 gcgtcggttc ctcctggcgg cgagtaccgt gtcttggggc ggccgttact ggcggaagcc
     3601 ctgtacgcgg cggtcaggct cgccgtcgcc gagtccacgc tcggagtgga ggcccggcgt
     3661 cggactgccg cgggccaggc ggccgccgaa gccgccacag ccgctaggat agaggcggcg
     3721 cctctgtccc cattcgggcg gaagctgctc ggagagggcc ccgcgatgtg caaggtccgg
     3781 gagcgcatcg agctctatgc cggcaatgag gcacccgtgc tgatcgtcgg cgagagcggg
     3841 accggtaagg aactcgccgc cgaagcgata cacctaggct cgcgccgtag cgctaggcgg
     3901 ttcctcccga tcgactgcgc cacgctgtcc gagagcctcg ccgaatcaac cctgttcggt
     3961 tcggtcaaag gcgcgttcac cgatgccgtc gataagcgcg gggccttcga gtcggcgcga
     4021 gggggcacgg tcttccttga cgagataggc gagctggctc tcacggttca ggccaagttc
     4081 ctgcggacgc tcgagtcggg aagcggctcg cgcgtaggct cggtggatca gaggcgctac
     4141 gatgtgcgga tcgtctcggc gacgaacgcc tcgagcctgg gcgacgccag gcgcttccgc
     4201 ccggagctgt tccaccgtat cagcacgctg gtgatagaga tgccagcgct cagggcgcat
     4261 aaggaggacc tgccgctgct cgtcggcgcc ctgctggccg agtacgcgtc cgagaagcac
     4321 gtcacggccg atacgatggc caagctatgg ggctgggatt ggcctgggaa tgtcagggaa
     4381 ctcaggaacg tcgtgaggag ggccgccctg ctatcgggcg gccgggagac gatactgccg
     4441 gccgacatcg aattcgactg cggcgcaagg ccgtggcaag gctgcctttt ctagacgtag
     4501 gtcggtcggg gtggcgtcag gacgcggtcc tggccgatac gacctcgagg tatacggcat
     4561 agccgccgtg tggcgacggc ccgagggtcg gcttccgcgc gacgaccacg gcccgcttgc
     4621 ctgccgagcc ttgcaggtac gaccggaaca cctcgcctcg gaatacgatg aacacccggt
     4681 gcaggagtag ggtcgacgga tccctccact cgccttgcat gaactcgacc actgcctcga
     4741 agggctcgtc gacggcgacg ctcggcgccg atagcgtgcc gaggagcagg atggcgcggt
     4801 cggacagggc cgccaaggcc gcctcgtcgc ccgtctccgc caaggccgag aggccggcga
     4861 tgctgacgcc gagttcgatc ctgccaacgt agctaggctc gccagccgga gccgactgcg
     4921 cggcggcaac cgtcagggcc aaggcggcga tcgccgtagc gagcatcatt cgtttcattt
     4981 cgccctccgt cgcgcggtga tccccgcccg tatacgagaa gcctactatc gcccggggcc
     5041 gcggtcaagc gagcctaagg cgccgagccg gatgaatagc gacgataggc gttccagctc
     5101 cgtctccgaa agggccgccc gagccgcgac ctcccgcagg aaggcctcgg cgtcgggccg
     5161 gccggccagc ttgtacaggc cggccgcctg catagcgtcg gccatcctgg cggccgcccg
     5221 cgcggcggac gagcgtggcg ccgccgttct gccgggccgg tcggtccctg gaccctcgcg
     5281 cgacgaggtg gccaggacgg cctcgcgtag ctcccagcag gcgatctgca cggcgtggga
     5341 caggttcagt gaggggaaga ggtccgaggt gtcgatgcgt acggcctcgt cgcagaggga
     5401 cagctcggcg tcggatagcc cgtcgcgctc gttaccgaat acgatggcgg ccgagct
//
LOCUS       DAOPHV010000114         5435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_19914_length_5435_cov_4.015985, whole genome shotgun sequence.
ACCESSION   DAOPHV010000114 DAOPHV010000000
VERSION     DAOPHV010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19914_length_5435_cov_4.015985"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..745
                     /gene="fdhD"
                     /locus_tag="PLQ29_04365"
     CDS             17..745
                     /gene="fdhD"
                     /locus_tag="PLQ29_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HPG85911.1"
                     /translation="MHEYKLADETPVELVANGERVAILLCTPINLDDLAVGHLFSRGM
                     LRDRAKVLAVGACADLRTMSVVAPGGVGPDRYGLGTVMATGAGPAAVRADASGLRTVG
                     SGLGVGMNELKAWARAMFEAAAIYRQSGGVHCAALVTRPSADGAPYFVVREDVGRHNA
                     VDKVLGRGFMDGVDFSSACALTSGRVAADMILKIISAGIPVIVSRSIPTTTAAELADA
                     AGVTIVGRIGDQSPVVYTRPERVV"
     gene            complement(742..2865)
                     /locus_tag="PLQ29_04370"
     CDS             complement(742..2865)
                     /locus_tag="PLQ29_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPG85912.1"
                     /translation="MYDSASPSEPSITALFGRNSFSNAVMREWLPKSCFVELLDVQAG
                     RKELSLEVADIVADAMKSWAIQNGATHYTHWFHPLNGLTAEKHESFIAPTGDGRVLME
                     FSGKELIKGEPDASSFPSGGLRATFEARGYTAWDVTSPAFLKQDATGTTLCIPTAFFS
                     YYGHALDKKVPLLRSMDALSKQAMRVLRALGNETSRCVIPTVGPEQEYFLVDKAYFDA
                     RPDLMLTGRTVFGSMPAKGQEMEDHYFGAINARVAAFMRTLNNELWKVGVAAKTQHNE
                     VAPNQFELAPIFASSNVAVDANQLVMETIKRAANQHGMEALLHEKPFDGVNGSGKHNN
                     WSIATDDGVNLLEPGDNPESNARFLLFITAIVEAVDRYAPLLRAAAAIAGNDHRLGAD
                     EAPPAIVSIFFGSLLSELLDSIADGKPLAGTGSAHRIELGVTTIPALPVDISDRNRTS
                     PFAFTGNKFEFRMVGSSQSIATPNTYLNTAVAQVLSEFADALEAAPDRLSAIQDIIRA
                     SYSSHRRVVFNGNGYSDEWIKEAERRGLPNVRSTVDALAILAREETIGLFETHKVLSR
                     AESVSRYHIYLEKYSKQVNIEAGVMADMARRTIFPATGAYAAALARDAAALAAIGAAS
                     AAIEKRAKRIAELAGDIAGDTERLEAILGEAQGIDDVGLRAKAYRDGVVPQMGALRGR
                     VDAAEKLVDKKAWPLPTYEELLFKL"
     gene            complement(3053..4669)
                     /locus_tag="PLQ29_04375"
     CDS             complement(3053..4669)
                     /locus_tag="PLQ29_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPG85913.1"
                     /translation="MIDRNDQYDELRVLRLLFGISRVLDRTPDLRGALAPMLDLLESE
                     AGLGRGVVTLLDRSLGVLRIEAAPSLSDEERGQGLYRLGEGLIGRVFETGAPVTVRDL
                     SAEPRLLNRAGCRAPGELAGVSCHVVPIHSGDTVIGTLSAERKAVVRPPASQAEPDDA
                     TLEADRSLLEKVAELLADSAKLRERLQEDRIKVGVDSAARADDGRYPGLGPSGPLPPS
                     LSGGIVGTSAAMRAVFELVTQVAPSDATVLVSGESGTGKELVAAELHRLSRRADGPFV
                     TVNCSALAESVIESELFGHERGAFTGAVGRRKGRFELAHGGTLFLDEIGELSPQVQIK
                     LLRVLQEREIERVGGSASLKVDVRVIAATNRRLDEEVKAGRFREDLYYRLNVFPIRIP
                     PLRERKSDIVLLADHFTEKYALRNDKPIKRISSPALDLFTSYLWPGNVRELENCIERA
                     VILSNDMVIHSYHLPPSLQSAGSTNTGPTTTLSAALSRLEKELIVEALKMSGGNMAQA
                     ARHLGITERQMGLRVRQYCLNWKQYRPTYS"
     gene            complement(4792..>5435)
                     /locus_tag="PLQ29_04380"
     CDS             complement(4792..>5435)
                     /locus_tag="PLQ29_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPG85914.1"
                     /translation="EANFALTGMAALLSALMHAPLTGIFLIAELTGGYELFVPLMIAS
                     ASAYGVHRAFSAHSIYTEDLAAENALVTWDKDAAVLLELDPYALVDEKAPRVSPREPL
                     SSIRRLLAERPAYLLAVVDEAGVYLGDLRFDDIRPLLFDDASGAREAGEFATKDPKTL
                     GLGLRPAEVLDSLDDARDGALPVVDGRGTFLGYLWREAALNEYRRLMIAMTKD"
BASE COUNT          893 a         1684 c         1847 g         1011 t
ORIGIN      
        1 agtgtaagga gagtcaatgc atgaatacaa gctggccgac gagacgcccg tagaactcgt
       61 cgccaacggc gagcgcgtgg ccatcctctt gtgcacgccg atcaacctcg acgacttggc
      121 cgtggggcat ctattctcgc gcgggatgct gcgcgacagg gccaaagtgc tcgctgtcgg
      181 cgcttgcgcc gaccttagga cgatgtcggt cgtcgccccc ggcggcgttg gtccagaccg
      241 atacgggcta gggacggtca tggcgaccgg cgctgggcca gccgccgtaa gggcggacgc
      301 gagcgggctt cgaactgtcg gatctggcct cggcgtgggc atgaacgagc tgaaggcctg
      361 ggcacgagcc atgttcgaag ctgccgccat ctatcgtcag agcggaggcg tacactgcgc
      421 cgccctggtg acgaggccgt ccgcggacgg ggctccgtac ttcgtcgtgc gcgaggacgt
      481 cggtcggcat aacgccgtag ataaagtgct cggtcgcggc ttcatggatg gcgtggattt
      541 ctcctcggcc tgcgcgctga cgagcggccg cgtcgcggcc gatatgatcc tcaagatcat
      601 atcggccggc attccggtca tcgtgtcgag gagcattccg acgacgaccg cggcggagct
      661 ggccgacgcg gccggcgtga ccatcgtcgg ccgcatcggc gaccagtcgc cggtcgtcta
      721 tactcgcccc gagcgcgtcg tctaaagctt gaacagcagc tcctcgtagg tcggcaaggg
      781 ccacgctttc ttgtcgacca gcttctcggc agcatcgact ctgccgcgca gggctcccat
      841 ctgcgggacg actccgtcgc ggtaggcctt ggcccgcagg ccgacgtcgt cgatcccctg
      901 ggcctcgccg agtatcgctt cgaggcgctc ggtatcgccg gctatatcgc cggcgagctc
      961 cgctatgcgc ttggcccgct tctcgatcgc cgccgacgcg gccccgatcg cggcgagggc
     1021 ggcggcatcg cgggccagag ccgccgcgta ggcgccggta gccgggaaga tcgtacgcct
     1081 ggccatgtcg gccatcactc cggcctcgat attgacctgc ttcgagtact tctcgagata
     1141 gatatggtag cggctgacgg actccgccct cgacagcacc ttgtgcgtct cgaacagccc
     1201 tatcgtctcc tcgcgcgcga gtatcgccag tgcgtcgacc gtcgagcgga cgttcggcag
     1261 ccctcgacgc tcggcttcct tgatccattc gtccgaatag ccgttgccgt tgaacacgac
     1321 cctgcggtgc gacgaatagg aagccctgat gatgtcctgt atagccgaga gccggtcggg
     1381 ggccgcctcg agcgcgtcgg cgaactcgga caggacctgg gcgaccgcgg tgttcagata
     1441 ggtgttcggc gtggctatgg actgggaaga gccgaccatg cggaactcga atttattgcc
     1501 ggtgaaggcg aaggggctgg tccggttgcg gtcggatatg tcgacaggca gggcagggat
     1561 cgtggtcacg ccgagctcta tccgatgcgc cgagccggtc ccggcgagcg gcttgccgtc
     1621 ggctatgctg tcgagcagct cggagaggag actgccgaag aatatcgata cgatcgccgg
     1681 cggcgcctcg tcggcgccga gccggtggtc gttgcccgct atcgcggccg ccgcgcgtag
     1741 gagcggagcg tagcgatcga ccgcctcgac gatggccgtg atgaacaaga ggaaacgggc
     1801 gttcgactct gggttatcgc caggctcgag taggttgacg ccgtcgtcgg tcgctatcga
     1861 ccaattgttg tgctttcccg agccgttgac gccgtcgaag ggcttctcgt ggagcaaggc
     1921 ttccataccg tgctggttgg ccgcccgctt gatggtctcc atcacgagct ggttcgcgtc
     1981 cacggcgacg ttcgagctgg cgaagatcgg cgccagctcg aattggttcg gcgcgacctc
     2041 gttatgctgg gtcttggcgg cgacgccgac cttccacaac tcgttgttca acgtgcgcat
     2101 gaaggccgca acccgcgcgt tgatcgcgcc gaagtagtgg tcttccatct cctggccctt
     2161 ggccggcatc gagccgaaga ccgtcctgcc ggtcagcatc aggtcgggcc tggcgtcgaa
     2221 ataggccttg tcgacgagga aatattcctg ctcgggcccg accgtcggga tgacgcaacg
     2281 ggaagtctcg ttgcccaggg cccggagcac gcgcatcgcc tgtttgctga gcgcgtccat
     2341 cgaccggagg agcggcacct tcttgtccag cgcgtggccg tagtagctga agaaggccgt
     2401 cgggatgcac agggtcgtgc ccgtggcgtc ctgcttgagg aaggcggggc tcgtcacgtc
     2461 ccaggcggtg tagcccctgg cctcgaaggt ggcgcgtagc ccgccggacg ggaagctcga
     2521 cgcgtcgggc tcgcccttga tcagttcctt gcccgagaat tccatcagca cgcggccgtc
     2581 gcctgtcggc gcgatgaacg actcgtgctt ctcggcggtc aggccgttga gcgggtggaa
     2641 ccagtgggtg tagtgggtcg cgccgttctg gatggcccag cttttcatcg cgtcggcgac
     2701 gatgtcggcc acctcgagcg acagttcctt gcggccggcc tgtacgtcga gcagttcgac
     2761 gaagcacgat ttgggtagcc actcgcgcat cacggcgttc gagaagctgt tgcggccgaa
     2821 taaggcggta atggatggtt cggatgggct cgcgctgtcg tacatgggtg ctcctgatgg
     2881 atcgaagatg ctatttttaa gcaagaagca tgccaggtaa tggtattttg aagtagaaga
     2941 gtccggatga ggctacgata cggtatggat aatgagaatt taagctctgc ggataatata
     3001 accagtgtga gatgagaata aactacattt gcgtaggaat tgtgcgttaa acctacgagt
     3061 atgtaggtct atactgtttc cagttcaggc agtactgacg gacgcgtagc cccatctgac
     3121 gctccgtgat acccagatgc cgggccgcct gggccatgtt cccgccgctc atcttgagcg
     3181 cctcgacgat cagctctttc tcgagcctgg acagggcggc tgacagggtg gtcgtcgggc
     3241 cggtgttcgt agatccggcg gactggaggc tcggaggcag gtggtacgag tgtatgacca
     3301 tgtcgttcga caggataacc gcccgctcga tacagttctc gagctcgcgc acgttcccgg
     3361 gccagagata gctcgtaaac aggtcgagcg ctgggctcga gatacgcttg atcggcttgt
     3421 cgttcctgag cgcgtacttc tcggtgaagt gatcggcgag caacacgatg tcgctcttgc
     3481 gctcgcgtag cggcggtatg cgtatcggga agacgttcag gcggtaatac aggtcctcgc
     3541 ggaaccgacc ggccttcacc tcctcgtcga gcctgcggtt cgtggccgct atcacccgca
     3601 cgtcgacctt gaggctggcg ctaccgccca cgcgctcaat ctcgcgttcc tggaggacac
     3661 ggagcagctt aatttggacc tggggcgaca gctcgcctat ctcgtcgagg aagagcgtgc
     3721 cgccgtgggc cagctcgaag cggcccttgc gccggcctac ggcgccggtg aacgcgccgc
     3781 gctcatggcc gaacagctcg ctctcgatca ccgactcggc caaggcagag caattgaccg
     3841 tgacgaaggg cccgtcggcc ctgcgcgaca atcggtggag ttccgcggcg accagctcct
     3901 tgcccgtgcc gctctcgccg gagacgagca ccgtcgcgtc gctcggggct acctgcgtca
     3961 ccagctcgaa cacggcgcgc atggccgcgc tcgtgccgac gatgccgccc gacaggctcg
     4021 gtggcagcgg ccccgacggt ccgagaccgg gatagcgccc gtcgtcggcc ctcgcggccg
     4081 agtccacgcc taccttgatg cgatcctcct ggagccgctc gcgtagtttc gccgaatcgg
     4141 ccagtagctc ggctaccttt tcgagcagcg atcggtccgc ctcgagcgtc gcgtcgtcgg
     4201 gctcggcctg gctcgcaggc ggcctgacta ccgctttccg ctcagcgctc agcgtgccga
     4261 tgaccgtatc gccggaatgt atcggtacga cgtggcatga aacgccggcc agctcgcccg
     4321 gcgctcggca gccggctcgg ttgagtagcc gcggctcggc tgacaggtct cgtacggtca
     4381 ccggggcacc cgtctcgaat acacggccga taaggccctc gccgagcctg tagaggccct
     4441 ggccgcgctc ttcgtcgctc aggctcgggg cggcctcgat acgcagcacg ccgagggatc
     4501 ggtcgagcag ggtgacgacg ccccggccga ggccggcttc cgactcgagt aggtcgagca
     4561 tcggcgcgag cgcgccccgc aggtcgggcg tccggtcgag cacgcgcgag atgccgaaga
     4621 gtagccgtag taccctgagt tcgtcgtatt ggtcgtttct atcgatcacg acgccatgat
     4681 gctacgaaaa tgttggaaag ttcaatgcct gttgtcctag cttcttccat tgacgcgagg
     4741 tgggagaagc cgtgggcggc ggcgaggcta tctccgtcga tgcgaggcga gtcagtcctt
     4801 cgtcatcgcg atcatcaggc ggcggtactc gttcagggcc gcctcgcgcc ataggtaccc
     4861 gaggaatgtt cctcggccgt cgacgacggg cagggcgccg tcgcgggcgt cgtcgaggct
     4921 gtccagcacc tcggccggcc tcaggccgag cccgagcgtc ttcgggtcct tggtagcgaa
     4981 ttcgccggcc tcgcgagcgc cggaggcgtc gtcgaagagc agggggcgaa tatcgtcgaa
     5041 gcggaggtcg ccgaggtaga cgcccgcttc gtcgaccacg gcgagcaggt aggccggccg
     5101 ctcggccagc aagcgtcgaa tcgacgatag gggttcccgg ggcgataccc tgggggcctt
     5161 ctcgtcgacg agggcgtagg ggtcgagctc caggagtacc gcggcgtcct tgtcccaggt
     5221 gacgagggcg ttctcggcag ccagatcctc ggtatagatg ctgtgggcgc tgaaagcgcg
     5281 gtggacgccg taggcgctgg cgctggcaat catcagaggg acgaacagct cgtagccgcc
     5341 cgtcagctcg gctatcaaga agataccggt caggggcgcg tgcatcaggg cagacaagag
     5401 ggcggccatg cctgtcagcg cgaagttagc ctcgg
//
LOCUS       DAOPHV010000115         5434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_19920_length_5434_cov_5.129764, whole genome shotgun sequence.
ACCESSION   DAOPHV010000115 DAOPHV010000000
VERSION     DAOPHV010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19920_length_5434_cov_5.129764"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLQ29_04385"
     CDS             complement(<1..253)
                     /locus_tag="PLQ29_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85915.1"
                     /translation="MLDETVIRLTVFRWLQDRDRWNGGIFSGRELNAGIVVEGRNVTL
                     KGQTGIWFPAGFTTPISITTSNQGPYELDDIGPDGLLTYA"
     gene            complement(482..1873)
                     /gene="purB"
                     /locus_tag="PLQ29_04390"
     CDS             complement(482..1873)
                     /gene="purB"
                     /locus_tag="PLQ29_04390"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPG85916.1"
                     /translation="MSDHSVYQSPFSWRYGSQSMRELWSDERKRRLWRSIWVSLARVE
                     SEYGLVTSAQVDELAAHAEELDVARSLEIEADIRHDLMAEVRAFAERCPTAGGVIHLG
                     ATSMDIEDNADALRLRDALGLVVGSLDRLLAALADKIDEYAGLPVMAFTHIQPAEPTT
                     LGYRLAVWAQDLLDDRERIARERDAVRGKGFKGAVGSSASYVELVGPENVGRFEERLA
                     EELGLGFYPIATQTYTRKQDYAVVSALAGLGASLHKFALDLRILQSPPIGELSEPFGK
                     HQVGSSAMPFKRNPINAEKIDSLARSLSVMPQVAWQNAAQSILERTLDDSANRRSMLP
                     EAFLICDEILATATRIVTGLVVSEEAIGRTMAAYGPFAATERVLMAMGKAGADRQEGH
                     ERLRRHALDAWAALRSGASNDLAERVASDPWFSGTLGRDGVLALMVADAHTGSAPGRA
                     REMAKAIRSEIGV"
     gene            1997..3022
                     /locus_tag="PLQ29_04395"
     CDS             1997..3022
                     /locus_tag="PLQ29_04395"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HPG85917.1"
                     /translation="MTVQRFLAPVPGLALAGALATVAYVAGKAAPIIGGPVFALAFGI
                     LVGLAPRPACFDSGQRLASKKTLQVAVALLGFGMDFGRALSVGSSSIVVMLSTLLASF
                     ATAFLVSRALRVRGKTVGLIAIGTAICGASAIAAAAPAMDADDDETAYAISTIFAFNV
                     AAVLIFPYLGRFLGLSPEGFGVWAGTAVNDTSSVVAVGMAFGPEALEVATVVKLTRTL
                     FIVPIVLALSARTARLARRSSSAATGPRAESALGRSASAFPWFVIGFVVASALGTSGI
                     VPADAASALASIGKALVLVAMAGVGLGVKPAMFRAAGWRPLALGLACWAAVAASSLAA
                     QAALGRW"
     gene            complement(3146..3802)
                     /gene="pcp"
                     /locus_tag="PLQ29_04400"
     CDS             complement(3146..3802)
                     /gene="pcp"
                     /locus_tag="PLQ29_04400"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: protein motif:HMM:NF009676.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HPG85918.1"
                     /translation="MRVLITGFEPFGDEAINPAWEAVKRLPDTISGASIVKLLVPTAF
                     GACGAILYEALERHAPDAIVGVGQAGGRCDLSVERVAINVADGRIPDNEGRLPVDEPI
                     QPDGPAAYFSTLPIKAIVARVRRRGVPASVSNSAGTYVCNYLMYLALHAVATGRPGTG
                     PGTLAGFVHVPYLPAQAVDKPATTPTMPVETMAAALEAAVEAIVEPGGATTAPEGTIC
                     "
     gene            3953..4462
                     /locus_tag="PLQ29_04405"
     CDS             3953..4462
                     /locus_tag="PLQ29_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPG85919.1"
                     /translation="MTLNAFAEARRFEAVVAVLDRHGRDPSKLIPILQAVQDEYRYLP
                     EEIMTYVATALDVSPAKVYGVATFYGHFSLEPKGKHVLKICDGTACHVKGSGALVDAL
                     RSELRLEGKAKTTPDLLFTLETVSCLGACGLAPVLVVGDDVHGLVTKDEALRIVRDIK
                     RAEASDGGN"
     gene            4449..>5434
                     /locus_tag="PLQ29_04410"
     CDS             4449..>5434
                     /locus_tag="PLQ29_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPG85920.1"
                     /translation="MAGTDTGRIRPGELEAIASAYAAASGAVRRRVVVCGGTGCIANG
                     STRVFERLRAALEAAGERVVVQLDVHDCSGASAHLSKSGCQGFCQVGPLVTIEPDGIL
                     YTRVKEADVDEIVASTFGRGEVVERLLYRDPVSGAAKRGKRDIDFYAKQERRVMHDCG
                     AIDPEDLNEYIARGGYRAARKAIFDLAPAEICALVKDSGLRGRGGAGFPTGRKWELAL
                     RQSSPDKYIICNGDEGDPGAFMDRSVLEGNPHAVIEGMLIGARATGATIGYVYVRAEY
                     PLAVQRVRKAVAAAMAAGFLGKDVMGSGIDFELRIMEGAGAFVCGEASAMVSS"
BASE COUNT          832 a         1775 c         1910 g          917 t
ORIGIN      
        1 acgcgtaggt cagaagtccg tcggggccga tatcgtcaag ttcatagggg ccttgattcg
       61 aggtggttat cgaaatcggc gtcgtgaacc cggcggggaa ccagatgccc gtctgccctt
      121 taagggtgac gttgcggccc tcgaccacga tcccggcatt gagctcgcga ccgctgaaga
      181 taccgccatt ccaacgatcg cgatcctgga gccatcggaa taccgtgagc ctgatcacag
      241 tctcatcaag cacgacgagc ctccatgaac cttatcccgg acaatcattg cgtaacgcgc
      301 ggacggacag ccattggaag agcgccatcg ggcggcggta gccatgtatc ctagccccgc
      361 cctaactata tggcaagctc tcgttaagga tcgaagcgtc aggcggctga tcgagtagca
      421 cctggaactc aagatagggt tcccgacggg gccgcggcgg gtggcaagtt caagccgttc
      481 cctacacacc gatctccgac ctgatcgctt ttgccatctc cctggcccgc ccgggggcgc
      541 tgcccgtatg ggcgtcggcg accatcaggg ccagaacccc gtcgcggccg agcgtcccgg
      601 aaaaccacgg gtcggaggcg acgcgctcgg ccaggtcgtt cgaggcgccc gaacggaggg
      661 cggcccaggc gtcgagcgcg tggcgcctga gccgctcgtg gccttcctgc cggtcggccc
      721 cggccttgcc catggccatc agcacgcgct cggtggcggc gaaggggccg taggcggcca
      781 tcgtccgccc tatcgcctcc tcggagacga cgaggcccgt cacgatcctc gtcgcggtcg
      841 ccagtatctc gtcgcagatc aggaaggcct cgggcagcat cgagcggcgg ttggccgagt
      901 cgtcgagggt gcgctccagt atcgactggg ccgcgttctg ccaggccacc tgcggcatca
      961 ccgacagcga gcgggccagc gagtcgatct tctccgcatt gatcgggttg cgcttgaacg
     1021 gcatcgccga cgagccgacc tggtgcttcc cgaacggctc ggacaattcg cctatcggcg
     1081 gcgactgcag gatacgcagg tcgagcgcga atttgtggag cgaggcgccg aggccggcca
     1141 gcgccgagac gaccgcgtag tcctgcttgc gcgtgtaggt ctgcgtggct atcgggtaga
     1201 agcccagccc gagctcctcg gctagccgct cctcgaaacg gccgacgttc tcggggccga
     1261 ccagctccac gtacgacgcg gagctgccga ccgcgccctt gaagcccttg ccgcgtaccg
     1321 cgtcccgctc gcgggcgatc cgctcccggt cgtcgagcag gtcctgggcc cagaccgcca
     1381 gccggtagcc gagcgtcgtc ggctcggccg gctggatatg cgtgaaggcc attaccggta
     1441 gcccggcgta ctcgtcgatc ttgtccgcca gcgcggcgag cagccggtca agagaaccga
     1501 cgaccaggcc gagggcgtcg cgaaggcgca gggcgtcggc gttgtcctcg atgtccatac
     1561 tggtcgcccc gaggtggatg acgccgccgg ccgtcgggca gcgctcggcg aaggcccgga
     1621 cctcggccat caggtcgtgg cgtatgtccg cctcgatttc gagggagcgg gcgacgtcga
     1681 gctcctcggc gtgggcggcc agctcgtcga cctgggccga ggtgaccagt ccgtactccg
     1741 actcgaccct ggccaggctg acccagatgg agcgccagag gcggcgcttg cgctcgtcgc
     1801 tccacagctc gcgcatggac tggctaccgt agcgccagct gaacggtgac tggtacaccg
     1861 aatggtcgct catgggcgga tagtagcaga ggacggcctg aggggctagc gggtcgccgg
     1921 gtctcgatcc gatgaccagg tcatcctcgg ctataatggt atttttttat gtatcgtatt
     1981 tcgttacact ctcggcatga cggtacaacg attcttggca cccgttccgg ggctcgcgct
     2041 ggccggtgcc ttggcgacgg tagcgtacgt cgccggcaag gccgcgccga tcatcggcgg
     2101 gccggtgttc gcgctggcct tcggcatcct ggtagggctg gccccgaggc cggcctgctt
     2161 cgattccggc cagcggctgg cgtccaaaaa aacgctacag gtagccgtag cgctgctcgg
     2221 attcggcatg gacttcgggc gggccctgag cgtaggctcg tcgtcgatcg tcgttatgct
     2281 atcgacgctc ctcgcatcgt tcgcgacggc tttcctggtc tcgagagcgc tgcgggtccg
     2341 gggtaagaca gtcgggctga tagcgatagg taccgccatc tgcggtgctt cggcgatagc
     2401 cgccgcggct ccggcgatgg acgcggacga cgacgagacg gcgtacgcca tatcgaccat
     2461 tttcgccttc aatgtggctg ccgtgcttat cttcccctac ctgggacggt tcctgggttt
     2521 gagccccgag ggcttcggcg tctgggccgg caccgcggtc aacgatacct cgtcggtcgt
     2581 cgctgtcggc atggcgttcg ggcccgaggc gctcgaggtc gcgaccgtcg tcaagctcac
     2641 gcgtacgctc ttcatcgttc cgatcgtact ggcgctatcg gcccggacgg ccaggctcgc
     2701 gcggcgttcc tcctcggcgg ctacggggcc gcgcgcggag tcagcgctcg gtcggagcgc
     2761 ttcggcgttc ccctggttcg tcataggctt tgtcgtggca tccgcgctcg gtacgtcggg
     2821 tatcgtgccg gccgacgccg cttcggcgct cgcctcgatc ggtaaggccc tcgtcctcgt
     2881 ggctatggcc ggggtcggcc tcggcgtcaa gccggccatg ttccgggccg ccggctggag
     2941 acccctggcg cttggcctgg cctgctgggc cgcggtcgcg gcctcctcgc tcgccgcgca
     3001 ggcggcgctc ggcaggtggt agctcggggc cgggcctaag gtgtcgatcg cgcaggcggc
     3061 gctcggcagt tggtagcccg ggaccgggcc taaggtgtcg atcgcgcagg cggcgctcgg
     3121 caggtggtag gcccgaccgg ccgtctcagc agatcgttcc ctcgggcgcg gtcgttgcgc
     3181 cgccgggctc gacgatggcc tcgacggcag cctcgagcgc ggcggccatg gtctcgactg
     3241 gcatcgtcgg cgtggtcgcc ggcttgtcga cggcttgggc cggtaggtac ggcacgtgga
     3301 cgaagccggc gagcgtgccc ggacccgtgc ccgggcgacc cgtcgccacg gcgtgcagcg
     3361 ccaggtacat caggtagttg cagacgtagg tgcccgcgct gttcgacacg ctggccggga
     3421 cgccccgcct gcggaccctg gcgacgatgg ccttgatcgg cagcgtcgag aagtaggccg
     3481 cggggccgtc gggctggata ggctcgtcca ccggtagccg cccctcgttg tcggggatac
     3541 ggccgtcggc cacgttgatc gcgacgcgct ccaccgatag atcgcaacgg ccgccggcct
     3601 gcccgacgcc tactatggcg tccggggcgt ggcgctcgag cgcctcgtac aggatggctc
     3661 cgcaggcgcc gaaggccgtc ggcacgagta gcttgacgat agaggcgccc gagatcgtat
     3721 cgggcagccg cttgaccgcc tcccaggccg gattgatcgc ctcgtcgccg aacggctcga
     3781 agccggtaat cagtacgcgc atcgctaacg ctcctcgccg caagtatagc atccatcctc
     3841 gcttgacatg atcgtcgatt gtatagtaaa aaatatatcg ataagacgta tcaagaatac
     3901 caccgacgag ccgcggccga gcggcggccg gcgagaacat aaggagtgat agatgacgtt
     3961 gaacgccttc gcggaggcca ggcgcttcga ggcggtggtg gccgtgctcg accgccacgg
     4021 ccgggatccg tccaagctga taccgatcct gcaggccgtg caggacgagt accgctacct
     4081 acccgaggag atcatgacct acgtggccac ggccctcgat gtatcgccgg ccaaggtcta
     4141 cggggtggct accttctacg gccacttctc cctcgaaccc aagggcaagc acgtgctcaa
     4201 gatctgcgac ggcacggcct gccacgtgaa gggctcgggc gcgcttgtcg acgcgctccg
     4261 ctccgagttg cggctggagg gtaaggccaa gacgacgccg gacctcctgt tcacgctgga
     4321 gacggtctcc tgtctcggcg cctgcggctt ggctccggtg ctggtggtcg gcgatgacgt
     4381 gcacggcctg gtcacgaagg acgaggcgtt gcgcatcgtc agggatatca agcgagcgga
     4441 ggcctccgat ggcgggaact gatacgggac ggatacggcc cggcgagctc gaggcgattg
     4501 cttcggccta cgcggccgcg agcggggcgg tgaggcggcg agtcgtcgtg tgcggcggta
     4561 cgggctgcat cgcgaacggc tcgacgcgcg tgttcgagcg cctgcgggcc gccctggagg
     4621 cggctggcga gagggtcgtc gtccagctgg acgtccatga ctgctcggga gcctcggccc
     4681 acctgtccaa gagcggctgc cagggcttct gccaggtggg gcccctcgtg acgatagagc
     4741 ccgacggcat cctgtacacc cgcgtgaagg aggccgacgt cgacgagatc gtcgcatcga
     4801 ccttcggccg tggcgaggtc gtcgagaggc tcctgtaccg cgaccccgtc tcgggcgcgg
     4861 ctaagcgggg caagcgcgac atcgacttct acgccaagca ggaacgccgc gtgatgcacg
     4921 actgcggcgc catcgacccc gaggacctga acgagtacat agcgcgcggc ggctataggg
     4981 cggcccgtaa ggccatcttc gacctggcgc cagccgagat ctgcgccctg gtcaaggatt
     5041 ccgggctgcg cggccgaggc ggcgccggct tcccgacggg ccgcaagtgg gagctggcgc
     5101 tcaggcagtc gagtcccgac aagtacataa tctgcaacgg ggacgagggc gaccccggcg
     5161 ccttcatgga ccgcagcgtg ctcgagggca acccgcacgc ggtgatcgag ggcatgctga
     5221 taggagcgcg cgcgaccggg gcgacgatag gctacgtcta cgtgcgcgcg gagtacccgc
     5281 tggcggtaca gcgcgtgcgc aaggccgtcg cggcggcaat ggcggcgggc ttcctcggta
     5341 aggacgtgat gggctcgggg atagacttcg agctgcgcat tatggagggc gccggcgcct
     5401 tcgtctgcgg cgaggccagc gcgatggtgt cgtc
//
LOCUS       DAOPHV010000116         5427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_19969_length_5427_cov_3.607781, whole genome shotgun sequence.
ACCESSION   DAOPHV010000116 DAOPHV010000000
VERSION     DAOPHV010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19969_length_5427_cov_3.607781"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PLQ29_04415"
     CDS             complement(<1..771)
                     /locus_tag="PLQ29_04415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPG85921.1"
                     /translation="MQTIMKYALVTGASGGIGKEFAGKLAAEGWAIILVGRNEGRLEA
                     VRAGLSGANASASVVVPCDLSQPGAAGRLYRACAERGLSVDLLVNNAGSGVFGDSLSQ
                     SPEAVEAMINLNVSSLTNLCALFGRDMKARGSGRILNVGSFAGLNATPYFASYAASKS
                     YVLDYTLALRAELSGSGVVVTCLLPGYVRTDFDDNAGIESEAYRKFSRSNSLGAEEVA
                     DIGLAALERGKAWTIAGARNRLAAFFFGLLPRTAPPAIM"
     gene            955..1248
                     /locus_tag="PLQ29_04420"
     CDS             955..1248
                     /locus_tag="PLQ29_04420"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPG85922.1"
                     /translation="MTIASIGEGDEFIVDSIRLCRETGKRLADMGFTKGARGRIVRRG
                     FLGGPLQVRLGDCDIMIRASEADGVPIEQVIGAGRGRGGRGRAGHRGWLANGR"
     gene            1280..3439
                     /gene="feoB"
                     /locus_tag="PLQ29_04425"
     CDS             1280..3439
                     /gene="feoB"
                     /locus_tag="PLQ29_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPG85923.1"
                     /translation="MRDDTSAGAKGDLRIALAGNPNAGKTTIFNALTGAHHKVGNYPG
                     VTVEKREGSRSRNGRRYDITDLPGIYSLTAYSIDEVVTRDFLLDHRPDIIVDVMDSTN
                     LSRHLYLCLQFQELGIPVIGALNMSDEAAAKGIRIDEKALGEVLGIPMIKTVGPKGSG
                     MDDLLDAIDRTADTMNGKTRKEVHYGAEIEEKLAALEAAVSADAAFIAKFPARWLAVK
                     LLEKDEHAFEKLAGHVDGAMIAARAKEASAWIDGHFGKDAEIVMSEQRYGYIRGAVKE
                     AITIEKKPDFSLTEAIDKVVMNRFLALPIFVGVLWSVFQITFALGEYPMAWFESLFAW
                     LGEAATTIMPDGFLRSLVVDGIIGGVGGVFSFVPLIVILFFLLSLLEDIGYMSRAAFA
                     TDKILHTFGLHGQSVFPMMLGFGCSVPAIMAARTLKSPRDRIVTILVIPFMSCGAKLP
                     VHVLLAAAFFPNNAGNMVMLIYAIGVLLALGSAWLLKKTVLRGEATPFVMELPPYRAP
                     TVRGVLWHVWEKTWHYIQKAGTIILASSVLIWAVTTFPSYEPTDSELASAEATFLAGS
                     PGATDDEVAARLETVTAELALANSAAGTFGRFIEPVFRPLGFDWKLGVATVTGFAAKE
                     VVVSTLGVLYSVGTEESEESEGLREALRNDPNWTPLLAFVMMLFTLVIPPCFAALAAI
                     KAELGWKWLGFAFAFMLGLGWALGAAIYQIGSLAGLA"
     gene            3436..3636
                     /locus_tag="PLQ29_04430"
     CDS             3436..3636
                     /locus_tag="PLQ29_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85924.1"
                     /translation="MSAVIQGAAVVLIVAAAAAYVLRNAVRALKGERRSCCDSGGAEP
                     AGGSSRPPCASCAGCSGCSMKR"
     gene            complement(3720..4739)
                     /locus_tag="PLQ29_04435"
     CDS             complement(3720..4739)
                     /locus_tag="PLQ29_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG85925.1"
                     /translation="MATIIGDAKSAVTALKQSSSELDAGMAEASEAARRIDQAIDGVI
                     ERTVNQAASVEEVSSTVEQITRNIESLDRMIERQKDGVAESSASIEEMVGAIGSIARN
                     VEAFGDYMKRLIEASDDGRGKLGGVSELVKDISTRSQGLSDANKVIQAIAARTNLLAM
                     NAAIEAAHAGEAGAGFAVVAAEIRSLAEQSQLRSKEIAGSVAGIRGGIDKVVASTVDA
                     ERAFENIVGHVRKVGELESEIKATVAEQGSGSRVVLESLSSIRDITDEVRGASAEMTQ
                     GAAAAGEEMRRLLELTEELKRSMQDIGKESESIKGVTAKVSALGVRNAELVGQVESGT
                     NRFKV"
     gene            4740..>5427
                     /locus_tag="PLQ29_04440"
     CDS             4740..>5427
                     /locus_tag="PLQ29_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85926.1"
                     /translation="MRILKTDAQGNLLWSQNFGLADYKEAGYGITVTANGDIVAVGER
                     SKTDNKIYVVRLNSAGTLQWENTYSIVAGESGQSIVETENGGFVIAGSTRIPNTSIQQ
                     GLLLKITGDGSPIPVWLHTFSSNAQILNDVENDGTGGFIVTGRTPEIVGLQNETYIAR
                     FSAQGSLIWENSAGKLGLSEGLAVARAINGGFVAVGYASTSTSPFQPQRYAYMVRTDA
                     DGNIFTNYLKG"
BASE COUNT          995 a         1762 c         1681 g          989 t
ORIGIN      
        1 catgattgct ggcggcgcgg tccgaggcag gagcccgaag aagaacgccg ccaaccggtt
       61 cctggcgccg gctatggtcc aggccttgcc ccgctcgagg gccgccaatc cgatatcggc
      121 cacttcctcg gcgccgaggc tgttcgaccg ggagaacttc ctgtacgcct cgctctcgat
      181 cccggcgtta tcgtcgaagt cggtgcgcac gtagccgggg agcaggcagg taacgacgac
      241 gcccgagccc gatagctcgg cccgcagggc cagcgtatag tcgagcacgt agctcttgct
      301 ggccgcgtac gaggcgaagt acggggtggc gttgaggccg gcgaacgagc cgacgttcag
      361 gatgcggccg gaaccgcgcg ccttcatatc gcggccgaac agcgcgcaga ggttcgtcag
      421 cgagctgacg ttcaggttga tcatcgcctc cacggcctcg ggcgactgcg acaggctgtc
      481 gccgaacacg ccggaaccgg cgttgttgac cagcaggtcg accgacaggc cgcgctcggc
      541 gcaggcccgg tacagccgcc cggccgcgcc cggctgagac aggtcgcagg gcacgaccac
      601 cgacgccgag gcgttggcgc ccgacaaacc ggctctgacg gcctccaacc tgccctcgtt
      661 ccgtcccacg aggatgatgg cccatccctc ggccgccagc ttgccggcga actccttgcc
      721 gataccgccc gaggcgcccg ttacgagcgc gtacttcatg atcgtctgca tcgctccttt
      781 atagactaga tacgcaagct aggaaagtat cattaagcgc ctgtccagcg ggtcagggag
      841 gcgccccgac gggcgtacga cctcggggtc ttgacagact acgttaaatt agataatgat
      901 aatcattatc atattgaatg cgaagccaac ggtattgtct tgagggagga atccatgacc
      961 atagcatcga tcggcgaagg cgacgagttc atcgtcgata gcatccgcct ctgccgcgag
     1021 accggcaagc ggctcgcaga catgggcttt accaagggcg cccgaggacg tatcgtgcgc
     1081 cgcggcttcc tcggcggccc gctccaggta cggctcggcg actgcgacat catgatacgc
     1141 gcgtccgagg ccgacggcgt gcccatcgaa caagtcatcg gagccggacg gggtcgcggg
     1201 ggacggggcc gtgcaggcca tcgcggctgg ctcgcgaacg gccgatgaac ggccgatgaa
     1261 cggcgaagga gccacgacga tgagagacga cacaagcgcc ggcgcgaagg gcgacctgag
     1321 gatagctctc gccggcaacc cgaacgctgg caagacgacc atcttcaatg ccctgaccgg
     1381 cgcccatcac aaggtgggta actatcccgg cgtgacggtc gagaagcgcg agggctcgag
     1441 gagccggaat ggccgccgtt acgacataac cgacctgcca ggcatctaca gcctgaccgc
     1501 ctactccatc gacgaggtcg tgacgcgaga tttcctgctc gaccacaggc cggacatcat
     1561 cgtcgacgtg atggactcga ccaacctatc gaggcacctc tacctctgcc tccagttcca
     1621 ggagctgggc ataccggtga tcggcgcgct gaacatgagc gacgaggccg cggccaaggg
     1681 catcaggata gacgagaagg ccctcgggga ggtgctcggc ataccgatga tcaagaccgt
     1741 cggccctaag ggctccggca tggacgacct gctcgacgcg atagaccgta cggccgacac
     1801 gatgaacggc aagacccgga aagaagtcca ttacggggcc gagatcgagg agaagctagc
     1861 cgcgctcgag gcggccgttt ccgccgacgc ggctttcata gccaagttcc ccgcgcgctg
     1921 gctagccgtc aagctactcg agaaggacga gcacgccttc gagaagctcg cgggccacgt
     1981 cgacggcgcc atgatagccg cccgggccaa ggaggccagc gcctggatcg acgggcactt
     2041 cggcaaggac gccgagatcg tgatgtcgga gcagcgctat ggctatatac gcggagcggt
     2101 caaggaagcc atcaccatcg agaagaaacc ggatttttcg ctgaccgagg cgatcgacaa
     2161 ggtcgtcatg aaccgcttcc tggccctgcc gatcttcgtc ggcgtactct ggtccgtgtt
     2221 ccagatcacc ttcgcgctcg gcgagtaccc gatggcctgg ttcgagtcgc tgttcgcctg
     2281 gctcggcgag gccgctacga cgatcatgcc cgatggcttc ctacgctcgc tcgtggtaga
     2341 cggtatcatc ggcggcgtcg gcggcgtctt ctcgttcgtg ccgctgatcg tcatcctgtt
     2401 cttcctcttg tccctgctcg aggatatcgg ctacatgtcc agggccgcct tcgcgaccga
     2461 taagatcctc cataccttcg gactgcacgg ccagtcggtc ttcccgatga tgctcggctt
     2521 cggctgctcg gtgccggcca tcatggcggc ccgcacgctc aagagcccgc gggaccgcat
     2581 cgtcaccatc ctcgtgatac ccttcatgag ttgcggcgcc aagctaccgg tgcacgtcct
     2641 gctcgccgcc gccttcttcc cgaacaacgc cggcaacatg gtcatgctga tctacgccat
     2701 cggcgtgctc ctggccctcg gctccgcctg gctgctcaaa aagaccgtcc tgcgcggcga
     2761 ggcgacgccc ttcgtgatgg aactgccgcc ctaccgggcc ccgaccgtgc gcggcgtgct
     2821 ctggcacgtc tgggagaaga cctggcacta tatccagaag gccggcacca tcatcctggc
     2881 ctcgtcggtg ctcatctggg cggtcacgac ctttccgtcc tacgagccga ccgattccga
     2941 gctggcgagc gccgaggcga ccttccttgc cggaagtccc ggcgccaccg acgacgaggt
     3001 ggcggcgagg ctcgagaccg tcacggcgga gctcgccctg gccaacagcg ccgccggtac
     3061 cttcggccgt ttcatcgaac cggtcttcag gccactcgga ttcgactgga agctcggcgt
     3121 cgccaccgtt accggcttcg cggccaagga ggtcgtcgtc tcgaccctcg gcgtgctgta
     3181 cagcgtcggt accgaggaaa gcgaggagag cgagggactg cgggaagccc tgaggaacga
     3241 cccgaactgg acgccgctcc tggccttcgt gatgatgctc ttcacgctgg tgataccgcc
     3301 gtgcttcgcg gcgctcgccg cgatcaaagc cgagctaggc tggaagtggc tcggcttcgc
     3361 cttcgcgttc atgctcggcc tcggctgggc gctcggggcc gctatctacc agatcggctc
     3421 gctggcgggg ttggcgtgag cgccgtcata caaggcgcgg cggtcgtact gatcgtggcg
     3481 gcggccgccg cctacgtcct gcggaacgcc gtccgggcgc tcaagggcga gcgccgttca
     3541 tgttgcgatt cgggcggcgc ggaaccagcc ggcggttcgt cgcgcccccc ctgcgcgagc
     3601 tgcgcgggtt gctccggctg ctctatgaag cgttagtggc gaaacgatgt aagaaccgta
     3661 ccaagccagc gacggcggcc gcgcgagcgg tcgccgtttt cggctgatcg ggacggttct
     3721 tacaccttga agcggttggt acccgactcc acctgaccta ccagctccgc gttgcgcacg
     3781 ccgagcgcgg acaccttcgc ggtcacgccc ttgatgctct cggactcctt gccgatatcc
     3841 tgcatgcttc gtttcagctc ctcggtcagc tcgagcaggc gccgcatctc ctcgccggcg
     3901 gcggcggcgc cctgggtcat ctcagcggag gccccgcgca cctcgtcggt tatatcgcgg
     3961 atcgacgaaa gcgactcgag caccacgcgg gagccggagc cctgctcggc cacggtggcc
     4021 ttgatctccg actcgagctc gccgaccttg cgcacgtggc cgacgatgtt ctcgaacgcc
     4081 cgctccgcat cgaccgtgga agcgaccacc ttgtcgatcc cgcccctgat gccagccaca
     4141 ctaccggcta tctccttgga acggagctgc gactgctcgg ccagggaccg gatctccgcg
     4201 gcgacgacgg cgaacccggc gcccgcctcg ccggcgtgcg ccgcctcgat ggcggcgttc
     4261 atcgcgagca ggttggtcct ggcggctatg gcctggatca ccttgttggc gtccgacaag
     4321 ccctgcgacc gggtcgagat gtccttgacc agctcggaga cgccgccgag cttgcccctg
     4381 ccgtcgtccg acgcctcgat cagccgcttc atatagtcgc cgaaggcctc gacgttcctg
     4441 gctatcgagc cgatcgcgcc gaccatctcc tcgatcgagg ccgacgactc cgcgacgccg
     4501 tccttctgcc gctctatcat gcggtcgagc gactcgatgt tcctggtgat ctgctcgacc
     4561 gtcgaggaga cctcctcgac gctggcggcc tggttgacgg tgcgctcgat gacgccatct
     4621 atggcctggt ctattctgcg agccgcctcg gacgcctccg ccatgcccgc gtccagctcg
     4681 gaggagcttt gcttgagcgc ggtcaccgcc gacttggcgt ctccgatgat ggtggccaaa
     4741 tgcgcattct taaaaccgac gcccagggca acttgctttg gagccagaat tttggccttg
     4801 cagattataa agaagcgggt tacggcatta cagtgactgc taacggtgac attgttgcgg
     4861 ttggcgagcg cagcaaaaca gacaataaaa tatatgtagt gcgcctcaat tccgcaggca
     4921 cgcttcaatg ggaaaataca tacagcattg tagctgggga atccggccaa agtattgttg
     4981 aaacggaaaa tggtggtttt gtgattgctg gatccacccg cattccgaat acctctattc
     5041 aacagggatt attactaaaa atcacgggtg atggcagtcc gattccggta tggctgcaca
     5101 ctttctccag caatgcacaa attctcaatg acgtagaaaa tgacggcacc ggaggattta
     5161 ttgtaacggg tcgcacccca gaaatcgtag gcttacaaaa cgaaacctat attgctcgtt
     5221 ttagcgccca gggaagcctt atatgggaaa attccgccgg aaaactgggt ctttctgaag
     5281 gtttggctgt tgcacgggcg atcaatggcg gttttgtagc ggtaggttat gccagcacct
     5341 ccaccagccc tttccaaccg caacgttatg cctacatggt acgcacggat gctgatggaa
     5401 atatctttac aaactatctg aaaggca
//
LOCUS       DAOPHV010000117         5416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_20009_length_5416_cov_4.633277, whole genome shotgun sequence.
ACCESSION   DAOPHV010000117 DAOPHV010000000
VERSION     DAOPHV010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20009_length_5416_cov_4.633277"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1178
                     /locus_tag="PLQ29_04445"
     CDS             120..1178
                     /locus_tag="PLQ29_04445"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPG85927.1"
                     /translation="MRRTTIAPFLAIPLFLSACVRCDTSVAADTGIVVMSYNLQTLFD
                     PVDQGGEYDGFVVADGDWTEAAYRSRIKALASAVLAATPPVGDSAADGPDVLVVQEAE
                     NARALRDLAEALGGYGYVLSSPDEEATLSCGILSRVPVTAFRAHRVRQPESSPSSVPR
                     YLLEAELDAGGRRLVVMAAHLKSKLGGAADTESERRAAAAFISSIVADRLAEDPTLAV
                     VVAGDFNENPDERERVGAAYPTALMPAKAGPGPWLLISGDRAGLEPRDGQPVLFCPWD
                     ESGGYSYRYDGADERIDQLLLSPGPLAAGDCPWAYSGFSAEPPEFAVDDSGQPLGWRN
                     GSGYSDHLPIRVSLSPSP"
     gene            1229..2908
                     /locus_tag="PLQ29_04450"
     CDS             1229..2908
                     /locus_tag="PLQ29_04450"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPG85928.1"
                     /translation="MKNRPLIAQSDQSLLLDAHDPAFDEARAELSAFATLEKSPEHFH
                     AYRLDALSLWNAASAGLSADAVVATLERWSRYELPASLGQAIRDTMARFGRIVMSEGP
                     DERTLRLACDDRFVRAELAGSKKLGKYLSPDGDGFLVALTERGTVKRELIKLGWPALD
                     TAPLLDGDPFPLALRATRSNGSPFSLRPYQEDAAAAFSGRGAPGTGYGVVVMPCGAGK
                     TVVGMATMAAIGRKTLIVTTNVAAVHQWMDELLDKTDISRDDLGEYTGAAKDVRPVTI
                     ATYQVLTWRPDKESDFPHFDLFRRERWGLIIYDEVHLLPAPVFRVVAEIQAIRRLGLT
                     ATLVREDGLQDDVFSLIGPKRYDVPWKELEAKGFIAEAFCREIRVPMADDERMRYVAC
                     GTRERHRVAAENPIKSKVVREILDNHPQDLVMVIGQFLDQLRTLAAELNAPLITGATP
                     NPERERIYADFRTGRVRTIVVSKVANFAIDLPDASVAIQVSGTFGSRQEEAQRLGRVL
                     RPKEKNSYFYSIVSRYTIEEEFADNRRRFLTEQGYRYTIEAWEEAEWAPTR"
     gene            2890..4956
                     /locus_tag="PLQ29_04455"
     CDS             2890..4956
                     /locus_tag="PLQ29_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85929.1"
                     /translation="MGADALNRWRDALLVDRDDTAIAAAKRWLGPVRTPFNKHELIAR
                     LEAFLKKPETASAVVGLLDRTERRILALALYSGTAPTDGLPRLELAKLASDEPAFEAS
                     SLERIRSLHDRLALYSYRDGRGRERVAVAPPLLGRLAAELSPSDALSSAKACESPRAP
                     DPFAAFCALVSACSHAKPAFKGRRELSKRASELLEAAIPGLAQDKERLAALLAGLEAC
                     GAFGVGDDGRPAAYPRRFVELCAEAGAAAPLALAAGCVWRGQALFGVHDDGHLVPATL
                     RASIEALPIGLAYSPADLRRLCAMAIRRRLEALAAAGVPGAEEALDHGLAGAVSATAE
                     ALGRLGVVALGGDGLVRTTSAAAALLDPPAGSGPGNVIVEESHELRILPEADPATRSF
                     IASVARLESAGLVWSAYLDKAAAKTAYAYGFRATGIAAELERLSGLPLPQSVRFSLDA
                     WEDESRSARLRVGIVLALDGHLAGMLEHSPKAEGLVRERLADGVYLLAAGDAAEAERL
                     LKAAGIDVDMRQDPRRDGGEARPLWASSSIDLSPSNATPPLAFRPRNDTLIDEGAPLV
                     ERLLAALDRLELPTEEKARFEAGVRARLVLGEEEFDRARAEEEPDSVGATDYPGKVRL
                     LEKAMKEGSRVELDYIDDTGRRLSAAGVPRDIRRTSSGALVTVSTDNGAQAVIPIRAI
                     TAARRT"
     gene            4979..>5416
                     /locus_tag="PLQ29_04460"
     CDS             4979..>5416
                     /locus_tag="PLQ29_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPG85930.1"
                     /translation="MRKTTDYDAGQLKLAVLIDADNTQPSIAEGLLAEVAKYGVASVK
                     RIYGDWTTTKLTQWKAKLLEYAIQPIQQFAYTSGKNATDSAMIIDAMDLLYTEKLDGF
                     CVVSSDSDFTRLAARMREAGKLVLGFGERKTPKPFVSAVDKFIY"
BASE COUNT          889 a         1904 c         1807 g          816 t
ORIGIN      
        1 gggctggtct ccaggatcgt gtcgacgagg gacgaactcg gcctctagcc ggggtcgcgc
       61 gccataagcg catgcgccat ggcgcggagc cccgttaccc atgcaggtaa gggggttcca
      121 tgcgccgtac caccatcgcg ccgttcctgg ccattccgct gttcctgtca gcctgcgtcc
      181 gttgcgatac gtccgtagcg gcggacacgg gcatcgtcgt catgtcttat aacctgcaga
      241 ccctgttcga cccggttgac cagggcggcg agtacgacgg cttcgtcgtc gccgacggcg
      301 actggaccga ggccgcgtac cgctcgcgca tcaaagcgct cgcctcggcg gtattggcgg
      361 ccacgcctcc ggtcggcgac tcggccgccg acggcccgga cgtgctcgtc gtgcaggaag
      421 ccgagaacgc cagggcgctc cgcgacctgg ccgaggccct cggcggttac ggatatgtcc
      481 tatcgtcgcc ggacgaggag gcgacgctat cctgcggcat actgtcgcgc gtccccgtga
      541 ctgccttccg agcccatcgc gtccgccagc ccgaatcatc gccatcgtcg gtgccgcgct
      601 acctgctcga ggccgagctg gacgcgggcg gaaggcgtct cgtcgtcatg gcggcccatt
      661 tgaagagcaa gctcggcggc gccgcggata ctgaaagcga gcgtagggcg gccgccgcct
      721 tcatatcgtc catcgtcgcc gacaggctcg cggaggaccc gacgctggcc gtcgtcgtgg
      781 ccggcgactt caacgagaac ccggacgaac gcgaacgcgt cggagccgcc tacccgaccg
      841 ctctgatgcc ggccaaggcg gggccgggcc cctggctcct gatatcgggc gaccgcgccg
      901 gactggagcc tcgcgacggc caaccggtgt tgttctgccc ctgggacgag tcgggtggct
      961 atagctaccg ctatgacgga gccgacgagc ggatagacca gctgctcctg tcgcccgggc
     1021 cgctcgccgc cggcgactgc ccctgggcct actcgggctt cagcgccgag ccgcccgaat
     1081 tcgccgtaga cgactcgggc caacccttgg gctggcgtaa cggctcgggc tactcggacc
     1141 acctaccgat acgggtatcg ctgtccccgt cgccctgagc cgccgcgtct ttgacagcgc
     1201 ggggcggcgc gtggtacaaa gaccggatat gaaaaaccgc ccgttgatcg cccagagcga
     1261 ccaaagcctg ctcctcgacg cgcacgaccc cgccttcgac gaggccaggg cggaactatc
     1321 ggccttcgcc accctcgaga agagccccga gcatttccac gcctaccgac tggacgcgct
     1381 atctctctgg aacgccgcga gcgccggcct atcagccgac gccgtcgtcg cgacgctcga
     1441 gcgctggtcc cgctacgaat tgccggcctc gctcggccag gccatacggg acacgatggc
     1501 ccgcttcggc cgcatcgtca tgtccgaggg accggacgag agaacgctca ggctggcctg
     1561 cgacgaccgc ttcgtccggg cggagctggc cggttccaag aagctcggga agtacctatc
     1621 gcccgacggc gacgggttcc tggtcgcgct gaccgagcgc ggcaccgtca aacgcgaact
     1681 gatcaagctc ggctggccgg cgctcgacac ggccccgctg ttggacggcg accccttccc
     1741 gctcgccctc agggcgacgc ggtccaatgg ctcgcctttc tcgctcaggc cctaccaaga
     1801 ggacgcggcg gccgccttct cggggcgcgg cgcgcccggc accggctacg gcgtcgtcgt
     1861 gatgccctgc ggcgccggca agaccgtcgt cggtatggcg acgatggccg ccataggccg
     1921 caagacgctc atcgtcacca cgaacgttgc cgccgtccat caatggatgg acgagcttct
     1981 cgacaagacg gacatctcgc gggacgacct cggcgagtac acgggcgcgg ccaaggatgt
     2041 gaggccggtc accatcgcca cctaccaggt gctcacctgg cggcccgaca aggaatcgga
     2101 ctttccgcac ttcgacctat tcaggcggga acgctggggc ctgatcatct acgacgaggt
     2161 gcatctgcta ccggctccgg tcttccgggt cgtcgccgag atccaggcca tacggcgcct
     2221 cggccttacc gccacgctcg ttcgcgagga cggcctccag gatgacgtct tcagcctgat
     2281 cgggcccaag cgctacgacg tgccctggaa ggagctcgag gccaagggtt tcatagccga
     2341 ggctttctgc cgcgagatac gcgtgccgat ggccgacgac gagcggatgc gctacgtcgc
     2401 ctgcggcacg cgggagcgcc accgggtggc cgccgagaac ccgatcaagt ccaaggtcgt
     2461 ccgcgagatc ctggacaacc acccccagga tttagtcatg gtcataggcc agttcctcga
     2521 ccagcttcgt acgctggccg ccgagctgaa cgcgccgctg atcaccggcg ccacgccgaa
     2581 ccccgagcgg gagcgcatct acgccgactt caggaccggc cgcgtgcgga ccatcgtcgt
     2641 atccaaagtg gcaaacttcg ccatcgacct acccgacgcc tcggtggcca tccaggtctc
     2701 gggtaccttc ggctcgcgcc aggaggaggc gcagcggctc ggcagggtac tgcgcccgaa
     2761 ggagaagaac tcctacttct actcgatcgt ctccaggtac acgatcgagg aggaattcgc
     2821 cgacaaccgc cggcgattcc tgacggaaca gggctaccgc tacacgatcg aggcctggga
     2881 ggaagccgaa tgggcgccga cgcgctgaac cgctggcgcg acgcgctgct ggtcgaccgc
     2941 gacgatacgg ctatagctgc ggccaagcgc tggctcggcc cggtcaggac gccgttcaac
     3001 aagcacgagc tgatagccag gctcgaggcg ttcctgaaaa agccggagac agcctcggcc
     3061 gtcgtcgggc tgctcgaccg gacggagcgc cggatactgg cgctcgccct gtactctggt
     3121 acggccccga ccgacgggct accgaggctc gagctggcca agctcgcctc cgacgagccg
     3181 gccttcgagg ccagctcgct cgagaggata cgaagcctgc acgaccggct cgcgctctac
     3241 agctaccggg acggcagggg acgcgagagg gtcgccgtgg cgccgccgct gctcgggcgg
     3301 ctggcggcag agctgtcgcc gtcggacgcg ctgtcgtcgg ccaaagcctg cgagtcgccc
     3361 cgtgcgcccg atcctttcgc tgccttctgc gcgctggtat cggcctgctc ccacgccaag
     3421 ccggccttca agggccggcg cgagctaagc aagcgggcgt cggagttgct cgaggccgcg
     3481 atcccgggcc tggcccagga caaggagcgc ctcgcggccc tactcgccgg gctcgaggcc
     3541 tgcggcgcct tcggcgtcgg ggacgacggg cgtccggccg cctacccgcg ccgcttcgtg
     3601 gaactgtgcg cggaggccgg ggcagccgcg ccgctcgcgc tcgctgccgg ttgcgtctgg
     3661 cgcgggcaag ccctattcgg cgtccacgac gacgggcacc tcgtaccggc gacgctacgc
     3721 gcttcgatcg aggccttgcc gatcggcctg gcctattctc cggccgactt gcgcaggctc
     3781 tgcgcgatgg ccatcaggcg gcgcctcgag gccctcgccg cggccggggt ccctggcgcc
     3841 gaggaggcgc tcgaccacgg cctggccgga gccgtctcgg ccacggcgga ggcgctcggc
     3901 cggctcggcg tcgtcgcgct cggcggagac ggccttgtca ggacaacctc cgcggccgcg
     3961 gccctgctcg acccgccagc cggatcgggc ccaggcaacg tcatcgtcga ggaatcccat
     4021 gagctccgga tcctgcccga ggccgacccg gcgacgcggt ccttcatcgc ctcggtcgcg
     4081 aggctcgaga gtgccggtct cgtctggtcg gcctacctcg acaaggccgc ggccaagacc
     4141 gcttacgcgt atggctttcg cgccacgggg atagcggccg agctggaacg cctctcgggc
     4201 ctcccgctac cgcagagcgt gcgcttctcc ctggacgcct gggaggatga gtcgcgttcg
     4261 gctaggctca gggtcggcat cgtcctcgcc ctggacggac acctggcggg catgctcgag
     4321 cattcgccga aggccgaggg tctcgtgcgg gagcgcctgg ctgacggcgt ctacctgcta
     4381 gcggccggcg acgccgcaga ggccgagcgc ctgctcaagg cggccggcat cgacgtagac
     4441 atgcggcagg accccaggcg ggacggcggc gaggccaggc cgctctgggc ctcctcatcg
     4501 atcgacctct cgccttccaa tgctacgccg ccccttgcgt tcaggccgcg gaacgataca
     4561 ctcatagacg agggcgcgcc gctcgtcgaa aggttgctcg cggctctcga tcggctcgag
     4621 ctaccgaccg aggagaaggc gcggttcgag gcgggcgtca gggcgaggct cgtgctcggc
     4681 gaggaggagt tcgaccgcgc ccgtgccgaa gaggagccgg actcggtagg cgccacggac
     4741 tatccgggca aggtccggct actcgagaag gctatgaaag aaggctctag ggtcgaactc
     4801 gactatatcg acgatacggg ccggagactc tcggccgctg gcgtaccgcg ggacatccgc
     4861 cgcacgtcat ccggagccct ggtaacagta tcgaccgata atggagccca agccgtcata
     4921 ccgatacgcg ccataacggc cgcgcgtcgg acgtaacaga ccgaatttgg agtaacgaat
     4981 gagaaagact accgattacg acgccgggca gctgaaactg gccgtgctca tcgacgccga
     5041 caacacgcaa ccgagcatcg ccgagggcct gctcgccgag gtggccaagt acggcgtggc
     5101 cagcgtcaag cgcatctacg gcgactggac gaccaccaag ctgacccaat ggaaggccaa
     5161 gctgctcgag tacgccatcc agcctatcca gcagttcgcc tacaccagcg gcaagaacgc
     5221 cacggacagc gccatgatca tcgacgcgat ggatctgctg tacaccgaga agctcgacgg
     5281 cttctgtgtc gtatcgagcg actcggactt cacgcgcctg gccgcgcgca tgcgcgaggc
     5341 gggcaagctc gtgctcggct tcggcgagcg caagacgccc aagcccttcg tgtcggccgt
     5401 ggataaattc atctat
//
LOCUS       DAOPHV010000118         5381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_20203_length_5381_cov_4.485167, whole genome shotgun sequence.
ACCESSION   DAOPHV010000118 DAOPHV010000000
VERSION     DAOPHV010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20203_length_5381_cov_4.485167"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="PLQ29_04465"
     CDS             <1..801
                     /locus_tag="PLQ29_04465"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG85931.1"
                     /translation="FLLTGPTGVGKTAFAERLAKALSGRDDALFRIDMSEFSDGHTAS
                     RLLGAPPGYIGYDDAPLLSKAVDACSGGVLLLDEFEKAHQQVHRVFLQILDAGRATDS
                     TGRTLSFSGLTIVATCNVGGDAGPALGFLAGTEAAALAASGTVPMAALRRAFPIELLN
                     RFDAIVPFRALDRDDARAILTGVVVRDANENLRREYGVELSFTDAALELLLAEGYSVE
                     LGARDLQRAFRRLVGVPLASMVSGLAGRGELRVDAAGATVSIAPTGVP"
     gene            834..1370
                     /locus_tag="PLQ29_04470"
     CDS             834..1370
                     /locus_tag="PLQ29_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85932.1"
                     /translation="MAKHVIDEVRGFYSIIELKPLKRGSDVALDIMPMEYLSHIDGIE
                     RVVHRRFALSLGSVGEVERPWYMHACQEDTLLVLHGSRSVELYAARHRRIESFVVEPE
                     RVVHDGETVFDGPAVLMWHAGVFHRSTTGDAGSASINFAARLEGFDPRKNYNIYDLDV
                     ATAKATLLRPGHLDTSPG"
     gene            complement(1381..1698)
                     /locus_tag="PLQ29_04475"
     CDS             complement(1381..1698)
                     /locus_tag="PLQ29_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPG85933.1"
                     /translation="MRAGKSPRFFCEHCGTEVGRDERACPVCGRFFSAVRCPRCGYSG
                     PAGRFAAGCPVCGYSMSPDGASDGLGSRPRRDGPAAPLPVWVWLLALGLLAAAVAALW
                     SIL"
     gene            complement(1695..2027)
                     /locus_tag="PLQ29_04480"
     CDS             complement(1695..2027)
                     /locus_tag="PLQ29_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPG85934.1"
                     /translation="MPTYEYECRACGYMFERVQSMSDEPVRECPECGHEVRRLIFGGS
                     GVIFKGSGFYVNDSKGKNPAAPKAKPGASESAEPKAAEAKSGSDGVAPSKPSVPADPK
                     PAKAKESV"
     gene            2404..2640
                     /locus_tag="PLQ29_04485"
     CDS             2404..2640
                     /locus_tag="PLQ29_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85935.1"
                     /translation="MYVIFEQRERDAPGAAFVDGAWDSFFRELDRLLPTYRAATAQAR
                     RANSPARAAGRPDEEREEPTDDASGRSFQAPGAA"
     gene            complement(2637..3740)
                     /locus_tag="PLQ29_04490"
     CDS             complement(2637..3740)
                     /locus_tag="PLQ29_04490"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPG85936.1"
                     /translation="MPARKPLSRAPRKAKPSGKRVGVLGAGAWGTAVAKALAENGHDV
                     TIWAREGELAAAINERHENGAYLPGVALPERLRASTEPLDAANGSEALFIAVPSPYML
                     QTVKRVLTSPDIMEGRTLVAVLTKGFVATERGPRLIVETLEDYLPGFYRGNLVYVSGP
                     SHAEEVSRGLMTGLAAASVNGRNAIRVRELLQSQSLLVFPSLDVIGVQVCGAVKNVVA
                     IAFGMLDALKAESDAFGDNTESMLLAAGLNEIQAFGQALGATHPETFTSIAGVGDLDV
                     TCRSIHGRNRRFGREIVQKGVLAPFANAADLIARIGELGYLPEGAPASGFVDRLAAEK
                     RLSMPICRGVHRILDKELTPREFIEEYLGSLSG"
     gene            3810..4790
                     /locus_tag="PLQ29_04495"
     CDS             3810..4790
                     /locus_tag="PLQ29_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85937.1"
                     /translation="MNIEFHYYAVYALALEAGFDERTAFILAASSQEVDASTTPLAFD
                     APRGRVDLAVTQNYLFWDEAVKRDVYLPFHFVPGDQAKAAFARADGLSSAYTVTPNSD
                     NVKELLVAAFKDKDPYLMGIAAHAFADSWAHQNFCGLQDPTNDIVASLAASSLPPAGH
                     LQALSSPDEPDVVWHDRRLKAGSERVVNRERFAAAAIKLYRYLRVFLGKPFSDDELVV
                     ARLGAIWAKPSKDERLADYVICWNIRPYEHGLWRREAGSPPDASPFAGVRHYDKLAWA
                     KAQLSRATGTGQAAAVRTDSSFYRSDLYRWHEAALEHRKRATALLRKKGL"
     gene            4787..>5381
                     /locus_tag="PLQ29_04500"
     CDS             4787..>5381
                     /locus_tag="PLQ29_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85938.1"
                     /translation="MMPRTKRRLSRAASTLIAAFATLAAGSCASVVRAPADVEAERLL
                     PVGAAAYARLDRAALSAVLASMPGVDANGASAIADRTDSMTLALVGAKDGSAVVVAVA
                     EGRYPAGAASMRLSSDPSWRKDGQAWSTRDGRLHLAFASSGRAFVGTAPLDAVLAAAA
                     EPNPQPVPARWADEWAAPIAVYLPRPMDALRARLPLGD"
BASE COUNT          825 a         1928 c         1775 g          853 t
ORIGIN      
        1 ttcctcctga ccggcccgac gggcgtcgga aagacggcct tcgccgagcg actggccaag
       61 gccctatccg gccgcgacga cgcgctgttc cgcatcgaca tgagcgagtt ctcagacggc
      121 cataccgcct cgcgcctcct cggcgcgcct cccggctaca tcggctacga cgacgccccg
      181 ctcctgtcca aggcggtcga cgcgtgctcc gggggggtgc tcctcctcga cgagttcgag
      241 aaggcccatc agcaagtgca ccgcgtcttc ctgcagatac tcgacgcggg ccgcgcgacc
      301 gattctaccg gcagaacgct gtcgttctcg ggcctgacca tcgtggccac ctgcaacgtc
      361 gggggagacg ccggccccgc gctcggcttc ctggccggca cggaggcggc cgcgctcgcg
      421 gcttcgggca cggtgccgat ggcggccctg cgtagggcct tcccgatcga gctgctcaac
      481 cgcttcgacg ccatcgtgcc gttccgtgcc ctcgacaggg acgacgctcg cgccattctg
      541 accggcgtcg tggtccgcga cgccaacgag aacctgcgac gcgagtacgg cgtcgagcta
      601 tcgttcacgg acgccgccct ggagctcctg ctcgccgaag gctactcggt cgagctgggc
      661 gcgcgcgacc tgcagcgcgc gttcaggcgc ctcgtcgggg tgccgctcgc ctcgatggtc
      721 tccggcctgg ccgggcgagg cgaactacgc gtcgacgccg ccggggctac cgtcagcatc
      781 gccccgaccg gcgttccttg attccatcgt cgccatgcgc aagactgtag cgtatggcga
      841 agcacgtgat agacgaggta cggggcttct atagcatcat cgagctcaag ccgctcaagc
      901 gcggttcgga cgtggcgctc gacatcatgc cgatggagta cctgagccac atcgacggta
      961 tcgagcgcgt cgtgcaccga cgcttcgcgc tgtccctggg ttccgtgggc gaggtcgagc
     1021 gcccctggta catgcacgcc tgccaggaag acacgctgct cgtcctccac ggctcgcgta
     1081 gcgtcgagct gtacgcggcc aggcatcgcc gcatcgagtc gttcgtggtg gagccggagc
     1141 gcgtcgtcca tgacggcgag accgtcttcg acggcccggc cgtgctgatg tggcacgccg
     1201 gcgtgttcca caggtcgacc acgggcgatg ccggttcggc gtcgatcaac ttcgccgcga
     1261 ggctcgaagg cttcgaccct cgcaagaact acaatatcta cgacctcgac gtggccacgg
     1321 ccaaggccac gctacttcgc cctggtcacc tcgacacgag tcccggctga cgagatccgg
     1381 ctagagtatc gaccagagcg ccgcgacggc agccgcgagc aggccgaggg cgagaagcca
     1441 gacccaaacg ggcagcggag ccgccgggcc atcgcggcgc ggccgggagc cgaggccatc
     1501 cgaggcgccg tcggggctca tgctgtaccc gcagaccggg caaccggccg cgaacctgcc
     1561 ggccggcccc gagtagccgc aacgcgggca acggaccgcc gagaagaagc ggccgcatac
     1621 tgggcaggcc cgctcgtcgc gccccacctc cgtcccgcag tgttcgcaga agaagcgggg
     1681 cgacttaccc gccctcagac gctttccttg gctttggcgg gcttcgggtc cgccggcacg
     1741 ctaggcttgg agggagcgac accgtcggaa ccggacttgg cctctgcggc cttcggctcg
     1801 gcggactcgg aggcgccggg cttggccttc ggggcggccg ggttcttgcc cttcgaatcg
     1861 ttgacgtaga aacccgaccc cttgaagatg acccccgagc ctccgaagat cagcctgcgg
     1921 acctcgtgtc cgcattcggg acactcgcgg accggctcgt cggacatgct ctggacccgc
     1981 tcgaacatgt aaccgcacgc tctgcattcg tattcgtacg tcggcatcgg aaacctcgtg
     2041 gtactctctt gatgatgatt gagtgagcat aggataccga caaaggacgg ttcgagtaaa
     2101 gtgtttctgt ccacttgccg cgcgcgccag tttctgagac tatgatgcgg gttcggacag
     2161 gccggtcgga tctcagggcc gctcccgcgg ccgacgacgg caatccagga cctcggcgca
     2221 cggaggcgct caatgatgat atcggtactc gacgccgcca aggcaggccg ctgaggggag
     2281 tctccccgac ttccaccgct accgctcccc tccgcatcat ctcataggta tcagacggta
     2341 aaaccggcgc tccggcgccg gaggatcgag cctagggccg atccagacgg aatggatccc
     2401 atcatgtacg ttatattcga gcaacgcgag cgagacgcgc caggcgcggc cttcgtggac
     2461 ggagcctggg acagcttctt ccgcgagctg gacaggctac tgcccacgta tcgcgcggcg
     2521 acggcccagg cgcggcgcgc caattcgccg gcgcgggccg ctggacggcc cgacgaggaa
     2581 cgggaggagc cgacggacga tgcgtccggc cgctccttcc aagccccagg cgccgcctag
     2641 cccgacaggc ttcccaggta ttcctctatg aactcgcgcg gcgtcagctc cttgtcgagg
     2701 atgcggtgca cgccgcggca aataggcatg gacaggcgct tctcggcggc taaccggtcg
     2761 acgaagccgc tcgccggcgc tccctcgggc aagtagccga gttcgcctat cctggctatc
     2821 aggtcggcgg cgttcgcgaa cggcgccagc acgcccttct gaacgatctc gcggccgaag
     2881 cgacggttgc gcccgtggat cgagcggcag gtcacgtcga ggtcgccgac cccggctatc
     2941 gaggtgaagg tctccgggtg cgtggcgccg agagcctgcc cgaaggcctg gatctcgttg
     3001 agcccggccg cgagcagcat cgactcggta ttgtcgccga acgcatccga ctccgccttg
     3061 agcgcgtcca gcatgccgaa ggctatcgcg acgacgttct tgaccgcgcc gcagacctgc
     3121 acgcctatca cgtcgagcga cgggaagacg agcaggcttt ggctctgcag gagctcccgc
     3181 acgcggatgg cgttgcgccc gttgacgctc gccgccgcga ggccggtcat cagcccccgc
     3241 gacacctcct cggcgtggct cggccccgag acgtagacca ggttgccgcg gtagaagccg
     3301 ggaaggtaat cctcgagcgt ctcgacgatc agcctcggcc cgcgctcggt cgcgacgaag
     3361 cccttggtca gtacggcgac gagggtacgc ccctccatga tgtccggcga ggtgagcacc
     3421 cgcttgaccg tctgcagcat gtacggcgac ggcaccgcga tgaacagcgc ctcgctgccg
     3481 ttcgcggcgt cgagcggctc ggtgctggcg cgtagccgct ccggcagggc gacgccgggc
     3541 aggtacgcgc cgttctcatg ccgctcgttg atggcggccg ccagctcgcc ctcgcgggcc
     3601 catatcgtca cgtcgtggcc gttctccgcc agggccttgg ccaccgccgt gccccaggcc
     3661 ccggcgccga gtacccccac ccgcttgccg gacggcttgg ctttcctggg cgccctcgat
     3721 agcggctttc gtgcaggcat atcgcctcct cggtatagtc ccggaccgtg catagccgcg
     3781 ggccgggcgt atgagtatct ttgcgatcga tgaatataga attccattac tacgcggtct
     3841 acgcgctcgc cctggaagcc ggcttcgacg agcggaccgc cttcatcctg gccgcctcgt
     3901 cgcaggaagt cgacgcgtcg acgaccccgt tggctttcga cgcgccgcgc ggccgcgtcg
     3961 acctggcggt aacccagaac tacctgttct gggacgaggc ggtcaagcgc gacgtctacc
     4021 tgcccttcca cttcgtgccg ggggatcagg ccaaggccgc ctttgcccgg gcggacggct
     4081 tatcgtcggc ctataccgtg acacccaact cggataatgt caaggaactg ctcgtcgccg
     4141 ccttcaagga taaggacccc tacctgatgg gcatcgcggc gcacgccttc gccgacagct
     4201 gggcccacca gaatttctgt ggcctccagg acccgaccaa cgacatcgtc gcctcgctcg
     4261 cggcctccag cctaccgccc gccggccatc tccaggccct gtcgtcgccc gacgagcccg
     4321 atgtcgtatg gcatgaccgc aggctcaagg ccggctcgga gcgggtcgtc aacagggagc
     4381 gattcgccgc cgcggcgatc aagctctacc gctacctccg ggtgttcctc ggcaagccct
     4441 tctccgacga cgagctcgtc gtcgccaggc tcggggcgat ctgggctaag ccgtccaagg
     4501 acgagcgttt ggcggattac gtgatatgct ggaacatacg gccctacgag cacgggctat
     4561 ggcgccgcga ggcgggctcg ccacccgacg cctcgccgtt cgccggcgtc aggcactacg
     4621 acaagctggc ctgggccaag gcccagctgt cgagggctac tggaaccggg caggccgcgg
     4681 ccgtacggac ggattcgtcg ttctaccgga gcgacctgta ccgctggcac gaggcggcgc
     4741 tcgagcaccg gaagcgcgcg accgcgctcc tgcgcaagaa aggactatga tgccgagaac
     4801 gaagcggagg ctatcacgcg cggcctccac cctcatcgcc gccttcgcga ccctggccgc
     4861 cggctcctgc gcgagcgtcg tgcgagcccc cgccgacgtc gaggccgagc gcctcctgcc
     4921 ggtcggcgcc gcggcctacg ccaggctcga ccgggccgcc ttgtcggccg tgctcgcctc
     4981 gatgcccggt gtggacgcca atggcgcgtc ggccattgcc gaccgcaccg actcgatgac
     5041 cctcgccctc gtcggcgcga aggacggttc ggccgtcgtc gtcgccgtgg cggagggccg
     5101 ctaccccgca ggggccgctt cgatgcggct gtcgtccgac ccgtcgtggc gcaaggacgg
     5161 ccaggcctgg tcgacccgcg acggcaggct ccacctggcc ttcgcctcgt cggggcgcgc
     5221 cttcgtcggc accgcgccgc tcgacgcggt gctggccgcg gccgcggagc cgaaccccca
     5281 gccggtgccc gcgcgctggg ccgacgagtg ggccgctccc atagccgtct atctgccgcg
     5341 cccgatggac gccctgcgcg ccaggctgcc gctcggcgac g
//
LOCUS       DAOPHV010000119         5375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_20239_length_5375_cov_3.853947, whole genome shotgun sequence.
ACCESSION   DAOPHV010000119 DAOPHV010000000
VERSION     DAOPHV010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20239_length_5375_cov_3.853947"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="PLQ29_04505"
     CDS             complement(<1..1370)
                     /locus_tag="PLQ29_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HPG85939.1"
                     /translation="MKKKQALAIAVFALFAIGQAWSQRKSADYYEMYAGEVTTLNYLI
                     SGSENEHIMFANVIDNLIEYDRYGVVQPGLAESWTTSPDGLVWTFKLRKNLRWYTWQG
                     KEYGDVTAQDWVDAAKYILTKANASRIVEPLSSVVKNADAYYEGKLSDFAQVGVRALD
                     KGTLEYTLEKPVPYFLSMLSYVTYLPVSGKFLAEAGAAFGRDQTRILYNGAYIMTEFE
                     PQVTRVLTKNKKYWDQKNVHIGRLIYRYNKEYATLAPELFLRGQTSYAAIPSTILDTW
                     MKDPAKKAQIHPARTSSYTYFYGFNFKPTFAAEYEPDNWKVAVNNKNFRKAVFHAFDR
                     KAAMLTAEPYAPSRRLQSTVTPRGFVSAGGLDYVATGSLLAIANGDSFDKAKALQYKA
                     AAMAELAGKATFPVKVMMPYNSSGSEWTNRVQVVEQQLEALLGKDFIDVIPVSFPPTG
                     FLNATRR"
     gene            1530..2552
                     /locus_tag="PLQ29_04510"
     CDS             1530..2552
                     /locus_tag="PLQ29_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG85940.1"
                     /translation="MLGCRFDRFYKEECRRDGRASGGQGQMLKYTVRRLLQSLVTLLI
                     VVSAVFLLMRLLPVEGYFGDGYDKLSESQREAVLQKMGLLDPWYVQLGSFYAGLLRGD
                     LGVSTIYRQDVPVLEVVAPRIPYSMRFGLVAIAIALVAGLAMGVSMAKHKGRALDKLW
                     TGYVVFVSAVPLAVYLLFIQLYVTELFGLPLLFDKYRPSSWILPALSMSLGSIAGYAM
                     WMRRYMVDELNKDYIRLAMAKGLPRSAIMVRHVMRNAYVPMAQYLPASILMTISGSIY
                     IESLYSIPGMGGLLVDVIQRQDNPMVQGLVLIFSSIGIVGLFLGDLLMALFDPRIKLA
                     KSEASR"
     gene            2549..3547
                     /locus_tag="PLQ29_04515"
     CDS             2549..3547
                     /locus_tag="PLQ29_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG85941.1"
                     /translation="MMRDRMSAGPMSPEAIRAAADGLDASLFEPAEFDASSGERGGYS
                     NYSYWRSTARVFIKNRVALVFLALLVTLLVFTFVQPLLPGQKSPTKIHIDPATGMQYR
                     NVEPGAEFWFGTNTIGQDLWSRIWSGTRTSLLIGLIVGAVEMVVGIAFGAVWGYVRRL
                     DGVITELYNVLNNIPTTIVLMLLSYILRPSFRTMVIAMCATGWLGLARFVRNQIVIIR
                     DREYNLASRCLGTPTRRIIFKNLLPYLVSVIMLQTALSIPGTIGWEVFLTYIGLGLPV
                     SIPSLGNLVNEGRQIMMSPELRYQLLFPTVVLSMITISFYVLGNAFSDASDPRNHV"
     gene            3559..4641
                     /locus_tag="PLQ29_04520"
     CDS             3559..4641
                     /locus_tag="PLQ29_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG85942.1"
                     /translation="MANGPAILEAKGLVIKFNLRGRTLTAIRGAGLELYEGETLAIVG
                     ESGSGKSVLTKSLIGMLDRNGWVDSGSAMFEGRDLARIKEERDWLGVRGKKISMVFQD
                     PMTALNPLLTIGAQIREVFELHHGLSGKKADHAVVEMLERVGIDNPEERRRQYPHEFS
                     GGMRQRVVIAIAVACKPRILICDEPTTALDVTIQAQILGLIKALQRDLAFTTVYITHD
                     LGVVANVADRVAVMYAGRIVETGLAEEVFYDSRHPYTWALLSSLPQLGVKGRPLYSIS
                     GTPPNLFNEVRGDAFAPRNPKALKVDFVAEPPEFEVSPTHRASTWLLDPRAPRIEPPP
                     AILAMRAKLGGAKLGGAKLGAVGGAL"
     gene            4638..>5375
                     /locus_tag="PLQ29_04525"
     CDS             4638..>5375
                     /locus_tag="PLQ29_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HPG85943.1"
                     /translation="MSGARETVLELRDLEVSFRLKGGGVFKAVKGVSFSIYKGETFGL
                     VGESGSGKTSIGRAIVRINDASSGSILYKGREISGRIPKELDREVVRKIQMIFQDPMA
                     SLNERAKVDYIVSEGLYNIGVKRSEGERRAMVAKALLEVGLLPEFASRFPHEFSGGQR
                     QRIGIARALIMEPELVVADEPISALDVSIRAQVLNLLSAIQKAKGLTYLFIAHDLSVV
                     RFITDRIAVINKGEIVELAETEELFRNP"
BASE COUNT          910 a         1723 c         1734 g         1008 t
ORIGIN      
        1 cgcctagtcg cgttgaggaa gcccgtcggc gggaatgaca ccggtatcac gtcgatgaag
       61 tccttaccga gcagggcctc gagctgctgc tcgacgacct ggacgcggtt cgtccactcg
      121 gagccgctgg agttgtacgg catcatcacc ttgaccggga acgtggcctt gccggccagc
      181 tcggccatcg cggcggcctt gtactggagc gccttggcct tgtcgaacga gtcgccgttg
      241 gcgatggcca gcagggatcc ggtggcgacg tagtcgagcc cgccggcgct cacgaagccg
      301 cgcggcgtca cggtcgactg gagcctgcgg ctcggcgcgt acggctcggc ggttagcatc
      361 gcggccttgc ggtcgaacgc gtgaaagacc gccttgcgga aattcttgtt gttgacggcg
      421 accttccagt tgtccggctc gtactcggcg gcgaaggtcg gcttgaagtt gaagccgtag
      481 aagtaggtgt aggagctggt ccgagccggg tgtatctggg ccttcttcgc cgggtccttc
      541 atccaggtgt ccaggatggt cgacgggatg gccgcgtacg aggtctgccc gcgcaggaac
      601 agctcggggg ccagagtcgc atactcctta ttgtagcgat agatcaggcg cccgatatgg
      661 acgttcttct ggtcccagta cttcttgttc ttggtcagca ccctcgtcac ctggggctcg
      721 aactcggtca tgatgtaggc gccgttgtat aggatcctcg tctggtccct gccgaaggcg
      781 gcgccagcct ctgccaggaa cttgccgctg accggtaggt aggtgacgta gctgagcatc
      841 gacaggaagt acggtaccgg cttctcgagg gtatactcga gcgtgccctt gtcgagcgcc
      901 cgtacgccga cctgcgcgaa gtccgatagc ttgccctcgt agtaggcgtc ggcgttcttg
      961 acgaccgagg atagcggctc gacgatgcgc gaggcgttcg ccttggtcag gatgtacttg
     1021 gccgcgtcga cccagtcctg ggccgtcacg tcgccgtatt ccttgccctg ccaggtgtac
     1081 cagcgtaggt tcttcctgag cttgaaggtc cagacgagtc cgtcgggcga ggtcgtccac
     1141 gattcggcga ggcccggctg cacgacgccg taccggtcgt actcgatcag gttgtcgatg
     1201 acgttggcga acatgatatg ctcgttttcc gagcccgaga tcaggtagtt gagggtcgtt
     1261 acctcgcccg cgtacatctc gtaataatcg gcggacttcc gctgggacca tgcctggccg
     1321 atcgcgaaca gcgcgaatac ggctatcgcc agagcctgtt tcttcttcat gcgtcgtttc
     1381 cttatccgat cagaaatcgt agggcggtaa caccgtcgtc gctagaagta ttccatcgat
     1441 agtaacgctc gtcaaacgaa acgtttcctc ccgtaggaac gatgccatga tccggccatg
     1501 aaatttcccg gcttcccatc ggccaaaccg tgttaggatg tcgcttcgac cgcttctaca
     1561 aggaggagtg ccgccgcgat ggccgggcca gcggcggaca gggtcagatg ctcaagtata
     1621 ccgtccggcg gctcctgcag tcgctcgtca ccctgctcat cgtcgtctcg gcggtgttcc
     1681 tgctgatgcg cctcctgccc gtcgagggct acttcggcga cggctacgat aagctcagcg
     1741 agagccagcg cgaggccgtg ctccagaaga tggggctcct ggatccctgg tatgtccagc
     1801 tcggctcgtt ctacgccggc ctcctgcgcg gggatctcgg cgtgtcgacg atataccgtc
     1861 aggacgtgcc ggtcctcgag gtcgtcgcgc cgaggatacc gtactccatg cgcttcggcc
     1921 tcgtcgccat agcgatcgcc ctcgtcgccg gcctggcgat gggcgtgtcg atggccaagc
     1981 acaagggccg cgcgctggac aagctctgga ccggctacgt cgtgttcgtc agcgccgtgc
     2041 cgctcgccgt gtacctgctg ttcatccagc tgtacgtaac cgagctcttc ggcctaccgt
     2101 tactgttcga caagtaccgg ccgtcaagct ggatactgcc cgccttgtcg atgtcgctcg
     2161 gcagcatcgc cggctacgcc atgtggatgc gacgttacat ggtcgacgag ctcaacaagg
     2221 actacatccg cctggcgatg gccaaggggc tgccgcgctc cgcgatcatg gtcaggcacg
     2281 tgatgcgcaa cgcctacgtg ccgatggccc agtacctgcc cgcgtcgatc ctgatgacca
     2341 tatccggctc catctatatc gagtcgctgt actcgatacc gggcatggga gggctcctcg
     2401 tcgatgtgat ccagcgccag gacaacccga tggtccaggg cctcgtgctc atcttctcgt
     2461 cgataggcat cgtcgggctg ttcctcgggg acctcctgat ggcgctcttc gacccgcgca
     2521 tcaagctggc caagagcgag gcctcgcgat gatgcgggac aggatgagcg ccgggccgat
     2581 gagccccgag gcgatccgcg ctgccgcgga cggcctcgac gcctccctgt tcgagcccgc
     2641 cgagttcgac gcgagctcgg gcgagcgcgg cggctactcc aactactcgt actggcgctc
     2701 gacggcgcgc gttttcatca agaacagggt ggccttggtc ttcctcgcgc tgctcgtcac
     2761 gctgctcgtc ttcaccttcg tgcagccgct cttgcccggg cagaagagcc cgaccaagat
     2821 tcatatagac ccggccaccg gcatgcagta caggaatgtc gagccgggcg ccgagttctg
     2881 gttcggcacg aacacgatag ggcaggacct ctggtcgagg atctggagcg gcacgcgcac
     2941 ctcgctcctg atcggcctga tcgtcggcgc cgtcgagatg gtagtcggca tcgccttcgg
     3001 ggccgtctgg ggctacgtgc gcaggctcga cggcgtgatc accgagctct acaacgtgct
     3061 caacaacata ccgacgacca tcgtgctgat gctgctgtcg tatatcctcc ggccgagctt
     3121 caggaccatg gtcatagcga tgtgcgcgac cggctggctg gggctcgcca ggttcgtgcg
     3181 caaccagatc gtcattatca gagaccggga gtacaacctc gcgtcgcgct gtctcggcac
     3241 gccgacgcgg cgtatcatct tcaagaacct gctaccctac ctggtatcgg tgatcatgct
     3301 gcagaccgcc ctgtcgatac cgggtaccat aggctgggag gtattcctga cctacatagg
     3361 cctgggcctg ccggtcagca tcccgtcgct cggcaacctg gtcaacgagg ggcggcagat
     3421 catgatgtcc ccggagctgc gctaccagct gctgttcccg acggtcgtgc tgtcgatgat
     3481 aaccatctcg ttctacgtgc tcggcaacgc gttctccgac gcgtccgacc cgcgtaacca
     3541 cgtgtaagga cggcggacat ggcgaacgga ccggcgatac tcgaggccaa gggcctcgtc
     3601 atcaagttca acctgcgcgg ccgcaccctg accgccatcc ggggcgcggg cctcgagctg
     3661 tacgagggcg aaacgctcgc gatcgtcggg gagtctgggt ccggcaagtc ggtgctgacc
     3721 aagtcgctca tcggcatgct cgaccggaac ggctgggtcg actcgggctc ggcgatgttc
     3781 gagggccgcg acctggcgag gatcaaggaa gagcgcgact ggctcggcgt gcgtggcaag
     3841 aaaatatcga tggtgttcca ggatccgatg acggcgctca acccgctcct gacgataggc
     3901 gcccagatcc gcgaggtctt cgagctgcac cacgggctgt ccggcaagaa ggccgaccac
     3961 gccgtcgtcg agatgctcga gcgcgtaggc atcgataatc ccgaggagcg gcgcaggcag
     4021 tacccccacg aattctccgg cggcatgcgc cagcgcgtgg tcatagcgat agcggtggcc
     4081 tgcaagccgc gcatcctgat ctgcgacgag ccgacgaccg cgctcgacgt gaccatccag
     4141 gcccagatcc tcggcctgat caaggccctg cagcgcgacc tggccttcac gacggtctac
     4201 atcacccatg acctcggcgt ggtggccaac gtggccgacc gggtcgcggt gatgtacgcg
     4261 ggtaggatcg tcgagaccgg gctggccgag gaggtgttct acgattccag gcacccgtat
     4321 acgtgggccc tcctgtcgtc gctgccgcag ctcggcgtca agggccggcc gctgtactcg
     4381 atatccggga cgccgcccaa cctgttcaac gaggtcaggg gcgatgcgtt cgcgccgcgc
     4441 aaccccaagg cgctcaaggt cgacttcgtc gccgagccgc cggaattcga ggtgagcccg
     4501 acccatcggg cgtccacctg gctgctcgac ccgcgcgcgc cgaggattga gccgcccccg
     4561 gccatcctcg cgatgcgcgc caaactcggc ggcgccaagc tcggcggcgc caaactcggc
     4621 gccgtgggag gagcgctatg agcggcgccc gcgagacggt gctcgagctg cgcgacctcg
     4681 aggtaagctt caggctgaag ggcggcggcg tcttcaaggc ggtcaagggc gtgtcgttct
     4741 cgatctacaa gggcgagacc ttcggcctcg tcggcgagtc gggctccggc aagaccagca
     4801 tcggccgcgc catcgtgagg atcaacgacg ccagctccgg ctcgatcctg tacaagggcc
     4861 gcgagatcag cggccgcatc cccaaggagc tcgaccgcga ggtggtgcgc aagatccaga
     4921 tgatcttcca ggacccgatg gcatcgttga acgagcgggc caaggtcgac tacatcgtct
     4981 cggagggcct gtacaatatc ggcgtcaaga ggagcgaggg ggagcgacgg gccatggtcg
     5041 ccaaggccct gctcgaggtc ggtctcctgc ccgaattcgc ctcgcgcttc ccgcacgagt
     5101 tctccggcgg ccaacgccag cgcatcggca tagcccgggc gctgatcatg gagccggaac
     5161 tggtggtggc cgacgagccg atctcggccc tcgacgtgtc gatacgcgcc caggtgctca
     5221 acctgctgtc ggccatccag aaggccaagg gcttgaccta cctgttcatc gcccacgacc
     5281 tgtcggtggt ccgcttcatc acggaccgta tcgcggtgat caacaagggc gagatcgtcg
     5341 agctggcgga gaccgaggag ctgttcagga acccg
//
LOCUS       DAOPHV010000120         5342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_20434_length_5342_cov_4.209003, whole genome shotgun sequence.
ACCESSION   DAOPHV010000120 DAOPHV010000000
VERSION     DAOPHV010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20434_length_5342_cov_4.209003"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLQ29_04530"
     CDS             complement(<1..399)
                     /locus_tag="PLQ29_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPG85944.1"
                     /translation="MAVAQIEFSKELQSFVETWKDKPGNLIMILHRVQQEFSYIPREA
                     AERLSRDLSMPLAKIYGVITFYHYFKTTKPGKYRMSICLGTACYLKGGQGLVDEAESI
                     LGIKGHEVTEDGLFSIDAVRCVGCCGLAPVL"
     gene            665..2236
                     /locus_tag="PLQ29_04535"
     CDS             665..2236
                     /locus_tag="PLQ29_04535"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPG85945.1"
                     /translation="MMAKSLSASAGVMLPAESPWLLAFWAALLAFALALGVYRKQTRV
                     YVAAASFFAAMAASQLRAMAPGLSGSLEAALGILSVAALGASRVALAHAADKACDGLT
                     RRSWRFASYAAAGLVIAIAVADSLLGPSLDATLQTGCVAVLAAHATLTIPFVVAAAKE
                     SPAAVRLLGGAIPWAIAWLAAAVLALARAFGLDLGVSLDGYAPIALAAADGLAGAALM
                     LAAMMELAKPAPEDTALDEYAAKMDASIKRFIPVEFLEHLEIQDFFDLKLGDHVRKDM
                     TIFFSDIRAFTELSEQLTPEESFAFINSYLSRVVPVINAHDGFIDKYMGDGIMALFAK
                     NGGADQAVESAVEMQAKMIEYNGHRANMNYRPISMGIGIHTGPLMLGVVGVSDRMEGT
                     VISDAVNLSSRLQSIAKAFNIPLVISERTFMQLDDPGRYKYRFIGKVRVKGKDAPVSV
                     FEIFDGLPEELFERKMKSNTFFEQGMMAYYQKDYSQGVFYFKRALESVPDDGASRFYL
                     ETCLRKAMSPKPTEK"
     gene            2248..4245
                     /locus_tag="PLQ29_04540"
     CDS             2248..4245
                     /locus_tag="PLQ29_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPG85946.1"
                     /translation="MNQPGLEFGVDWYPEHWDENRWRTDVERMAGYGFSCVRLMEFAW
                     TIVEPEPGRFDFSLFDRAIGLLAEAGLKAIVGTPTATPPHWLSDRPVFRVSPTGASHA
                     YGTRRNLCYNAPEYREASRRVVAAIAKRYGGDTRVAGFQIDNEIGHETSDRCVCEHCR
                     HEWPHWLERRYGDIASLNRAWGAVFWNTSYASFDQVPMASIQPSTIQNPALVLDYDRF
                     SSDSAVSFAREQTDALRAASSPGQWVTTNLFPPPLSNAIDMEALTDGMDFASWDNYPV
                     WGDQDEPLPWQFNATAQAYVRGLRDNVPFTVMEEMSGFQGHVCLGHLPPERQVALWAV
                     QAVARGANRVVFFRWRTAPFGQEQLCHGLVDGDDRETERLRVIAATMRRAKAELSSLA
                     SVPVESPVCLAYSKDDARVLREQYLSKGLLLRVSPTVQAGYDKELASWYAPFPTLGVG
                     ADAAPIGSLEPERYRVISLPLYQMADSAVVDRLGHWVESGGSLVLGYRAGARDALDHN
                     VEATLPGLFAELAGIRVPRFESLNETSAALRVGVVPGRGGVWADIIEPDGAETVAVWT
                     DRSKFYRGSPAVTVNRVGKGLVWYLGTSPNPIAMLALYRRILKAAGLCPRFAGADVEA
                     IERRDTDGKTWRLVLNHSPRRRRAYGATLEPWGWVKLPVSE"
     gene            complement(4415..>5342)
                     /locus_tag="PLQ29_04545"
     CDS             complement(4415..>5342)
                     /locus_tag="PLQ29_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85947.1"
                     /translation="AVVAASGSDIDNAAIKLERNANKARAVLAKLRFVAVDAPTAAGL
                     GKDYAKPFKARLVFGEGDSAPGIPGAEVYVAYQRRQSSGRVVTRTERAMTDADGVVSF
                     APPPPDFVGKASLRFSLNLDSTSELLERVPAAYDAYVAAIADELARRSVTFDYVVSSA
                     AKTVPTGVVIVDLGDDGQPLGTTIAQGGLFETLAKEKFKAGLAPLSAAMIASFDDAAI
                     LKAAKAAYGAGLSRLIYGAARIERSEKDGSMWQATARMTVRCVDFATGAILYSGEKTA
                     VSVAADEASAKRSALLQVARDGVAKDLMANLP"
BASE COUNT          942 a         1738 c         1691 g          971 t
ORIGIN      
        1 cagcaccggc gcgaggccgc agcagccgac gcacctgacc gcgtcgatcg agaagagccc
       61 atcctcggtg acctcgtggc ccttgatgcc caggatgctc tccgcctcgt cgacgaggcc
      121 ctggcctccc ttgaggtagc aggcggtgcc gaggcagatg gacatgcggt acttaccggg
      181 cttggtggtc ttgaagtagt gatagaacgt gatcacgccg tagatcttag ctaagggcat
      241 gctcaggtcc ctcgacagcc gttccgccgc ttcgcgcgga atgtagctga actcctgctg
      301 gactcgatga aggatcatga tcaagttacc aggcttgtcc ttccacgttt ccacgaagct
      361 ctggagttct ttcgagaact cgatttgagc cacagccatt atattccccc cttgttgatc
      421 gatatatata tatcgatagt aacagcggtt aattgtaccc ggtcgtccca taaacttcaa
      481 tcgggtatgg tcctagaaac gccaaaaagc acttaaaatt ttagtattta atctcgaaat
      541 ttcatatagc gaaatatacc aaagcgaaga agcgtgaaac gcggacgaga gccgcggcgt
      601 tccagaggct gtccgagggc tgatggcgat ggaaaagaac ggggtctgct gttattatag
      661 gagcatgatg gcaaaaagtc taagcgcttc cgccggggta atgctcccgg ccgagtcgcc
      721 atggttgctg gccttttggg cggcgttgct cgcgtttgcc ctggccctag gcgtttatcg
      781 gaagcagacg cgcgtctacg tcgccgcggc gtcgttcttc gcggccatgg cggcgtcgca
      841 gctacgcgcc atggcaccag gactgtcggg ctcgctcgag gccgcgctcg ggatcctatc
      901 cgtcgcggcg ctcggcgcgt cgagggtggc cctggcccac gcggccgaca aagcctgcga
      961 cggcctgact cgccgttcat ggcgtttcgc ttcctacgcc gcggccgggc tcgtgatcgc
     1021 gatcgccgtg gccgattccc tgctcggccc atcgttggac gcgaccctcc agaccggctg
     1081 cgtcgcggtt ctggccgccc acgccacgct taccatcccc ttcgtcgtcg cggcggccaa
     1141 ggaatcgccg gccgccgtcc ggctactcgg cggggccata ccctgggcta tcgcctggct
     1201 ggccgccgcc gtcctggccc tggcgcgcgc attcggcctg gatctcggcg tttccctcga
     1261 cggctacgcc ccgatagctt tggcggcggc cgacggcttg gccggcgccg ccctgatgct
     1321 cgccgcgatg atggagctgg ccaagccggc gccggaggat accgccctcg acgagtacgc
     1381 cgccaagatg gacgcatcca tcaagcgctt catccccgtg gaattcctcg agcacttgga
     1441 aatacaggac ttctttgacc tgaagctcgg cgaccatgta cgtaaggaca tgacgatatt
     1501 cttctcggac atccgcgcct tcaccgagct atcggagcag ctgacgccgg aagagagctt
     1561 cgccttcatc aattcgtacc tgtcccgcgt ggtgcccgtc atcaacgccc acgacggctt
     1621 catcgataag tacatgggcg acggcatcat ggcgctgttc gccaagaacg gcggcgccga
     1681 ccaggccgtc gagagcgccg tggagatgca ggccaagatg atcgagtaca acggccaccg
     1741 ggcgaacatg aattataggc cgatctcgat gggcataggc atccacacgg ggccgctgat
     1801 gctcggcgtc gtcggcgtct ccgaccggat ggagggcaca gtcatctccg acgctgtcaa
     1861 cctatcgtcg cgccttcaga gcatagccaa ggccttcaat ataccgctcg tcatcagcga
     1921 acgcaccttc atgcagctcg acgacccggg gcgctacaag taccgcttta tcggcaaggt
     1981 gcgcgtcaag ggcaaggacg cccccgtatc ggtcttcgag atcttcgacg ggctacccga
     2041 ggagctgttc gagcgcaaga tgaagtcgaa caccttcttc gagcagggca tgatggccta
     2101 ctaccagaag gactactcgc aaggcgtctt ctacttcaag cgcgccctgg agtcggtgcc
     2161 cgacgacggc gcctcccgct tctacctgga aacctgcctg cgtaaggcca tgtcgccgaa
     2221 gccaactgag aaatgatggg ggatacgatg aaccaacctg ggctcgaatt cggcgtcgac
     2281 tggtatccgg aacattggga cgagaaccgg tggagaaccg acgtcgagcg catggccggc
     2341 tacggcttct cgtgcgtccg gctgatggaa ttcgcctgga ccatcgtcga gccggagccc
     2401 gggcgcttcg acttctcgct gttcgaccgg gccataggct tattggcgga ggccgggctc
     2461 aaggcgatcg tcgggacgcc gaccgcgacc cctccccatt ggctgtcgga tcgcccggtc
     2521 ttccgggtat cgccgaccgg tgcgtcccac gcctacggca ccaggcgcaa cctgtgctac
     2581 aacgcgcccg agtatcggga ggcgtcccgg agggtcgtcg cggcgatcgc gaaacgctac
     2641 ggcggcgaca cgcgggtggc cggtttccag attgacaacg agataggcca cgagacgtcg
     2701 gatcgctgcg tctgcgagca ttgtcgccac gagtggccgc attggctcga gcggcgctac
     2761 ggcgacatcg ctagcctgaa tcgcgcctgg ggcgccgtat tctggaatac gagctacgcg
     2821 agcttcgacc aggtacccat ggcgtccatc cagccatcga cgatacagaa cccggccctg
     2881 gtgctcgact acgaccgctt cagctccgac tcggccgtca gcttcgcgag ggagcagacc
     2941 gacgccctgc gcgccgcttc gtcccccggt cagtgggtca cgacgaacct gttcccgccg
     3001 cccctgtcca acgcgatcga tatggaagcg ctgacggacg gcatggactt cgcctcgtgg
     3061 gataactacc cggtctgggg cgaccaggat gagccgctac cgtggcagtt caacgcgacc
     3121 gcccaggcct acgtccgagg actacgggac aacgtcccgt tcacggtgat ggaggagatg
     3181 tcaggcttcc agggccacgt ctgcctcggt cacctgcctc cggaacgcca ggtcgctctt
     3241 tgggccgtcc aggccgtcgc ccgcggcgcg aaccgcgtcg tattcttccg ctggcgcacc
     3301 gctcccttcg gacaggagca gctgtgccac ggcctcgtcg acggcgacga ccgggagacg
     3361 gagcggctac gggtgatcgc cgcgaccatg cgccgcgcca aggccgagtt gtcgtctctg
     3421 gcttcggtcc cggtcgagag tcccgtctgc ctagcgtatt ccaaggacga cgcgagagtc
     3481 ctgcgggagc aatacctgtc caaggggcta ctccttcgcg tctctccgac ggtccaggct
     3541 ggctacgaca aggagctcgc ctcgtggtac gcgccgttcc cgacgctcgg agtcggcgcc
     3601 gacgcggccc cgataggatc gctcgagccc gagcgctacc gcgtcatatc gctaccgctc
     3661 taccagatgg ccgactccgc agtggtcgac cggctcggcc attgggtcga aagcggcggt
     3721 agcctggtcc tcggctatcg agccggcgcc cgggacgccc tcgaccataa cgtcgaggcg
     3781 accctaccgg gcctgttcgc cgagctggcg ggcatcaggg tgccacgctt cgagtcgctc
     3841 aacgagacga gcgccgccct gcgcgtcggc gtcgtgccag gccgcggcgg cgtctgggcc
     3901 gacatcatcg agccggacgg cgccgagacg gtggccgtct ggaccgaccg ttcaaagttc
     3961 tatcgtggca gcccggccgt caccgtcaac cgggtcggca agggcctcgt ctggtacctc
     4021 ggcaccagcc cgaacccgat agcgatgctg gccttgtacc ggcgtatcct caaggccgcc
     4081 ggcctgtgcc cgcgtttcgc cggcgcagac gtagaggcta tcgagcgccg cgacaccgac
     4141 ggtaagacgt ggcgactggt cttgaaccat tcgccgaggc gcagacgagc ctacggggcg
     4201 acgctcgaac cctggggctg ggtcaagttg cccgtcagcg aatagacgga ataaaacgga
     4261 tttgccgagg cgacgaagag gaaaaaggat acgagatacg aaaggggaag tctgaagaaa
     4321 gagctttaag gcttttggtc ctttcaaagc ttttctcttt ctctcctcta atactcgtat
     4381 cctcttcacc gccttaggcg tttagatcta tcgtttacgg cagattggcc atcagatcct
     4441 tggccacgcc gtccctggcc acctggagca gggccgagcg ttttgcactc gcctcgtcgg
     4501 cggcgaccga gacggcggtc ttctcgccgc tatacaggat ggccccggtg gcgaagtcga
     4561 cacagcgcac cgtcatcctc gcggtagcct gccacatcga gccatccttc tcggagcgct
     4621 ctatcctagc cgcgccgtag atcagcctag agagtccggc cccgtacgcg gccttggccg
     4681 ccttgaggat ggccgcatcg tcgaaggacg cgatcatcgc ggcgctgagc ggggctaagc
     4741 ctgccttgaa cttttccttg gccagagtct cgaagagacc gccctgggca atggtcgtgc
     4801 cgaggggctg gccgtcgtcg ccgaggtcca cgatcacgac gccggtcggc accgtcttgg
     4861 cggccgacga tacgacgtag tcgaaggtca cggagcgtcg ggccagctcg tccgctatgg
     4921 ccgcgacgta ggcgtcatag gccgcgggca cgcgttcgag cagctcgctc gtcgaatcaa
     4981 ggttcagcga aaagcgcagg ctcgccttgc cgacgaagtc gggcggcggc ggggcgaagg
     5041 acaccacgcc atcggcgtcg gtcatggcgc gctcggtcct ggtcacgacg cgtccggagc
     5101 tctgccttcg ctggtaggcg acgtacacct cggcgccagg tatgcccggg gcggagtcgc
     5161 cctcgccgaa gacgagcctg gccttgaacg gcttcgcgta gtccttgccg agaccggccg
     5221 cggtcggcgc gtcgacggcg acgaagcgca gcttggcgag cacggcgcgc gccttgttgg
     5281 cgttgcgctc cagcttgatg gcggcgttat cgatatcgga gccggaggca gcgacgacgg
     5341 cc
//
LOCUS       DAOPHV010000121         5341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_20439_length_5341_cov_4.492622, whole genome shotgun sequence.
ACCESSION   DAOPHV010000121 DAOPHV010000000
VERSION     DAOPHV010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20439_length_5341_cov_4.492622"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PLQ29_04550"
     CDS             <1..682
                     /locus_tag="PLQ29_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPG85948.1"
                     /translation="FLAAKGIAPGLPPIVGALVFAVIAAAVIGFVSLRAKQREDTVIN
                     AIWAIGMSVGVLFIAKTPGYADPMSYLFGNILLVSGRDLALLAALDAVVLFLAWRFYP
                     QIEASAFDEEFAKVRGVPTDAVFVATLAVTAVAVVLLQTFVGIVMVIAMLTLPSGTAG
                     FAARSLAAMMAGGAALSAAFTVAGLAASWSLDLPAGAVVVVIAGAAFLAVSAAVAVKA
                     RKARGGRA"
     gene            679..1059
                     /locus_tag="PLQ29_04555"
     CDS             679..1059
                     /locus_tag="PLQ29_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPG85949.1"
                     /translation="MTRARAAVLAALESTVEPLSAAGVASRMAGACDQATVYRALHHL
                     EEAGLAESFVLHCAMHGVERYYAATSSPHRHWFHCESCHRFVDLGACRLGGLVSELER
                     ERGLRVTRHTMYLTGVCGDCAARE"
     gene            complement(1107..2930)
                     /locus_tag="PLQ29_04560"
     CDS             complement(1107..2930)
                     /locus_tag="PLQ29_04560"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HPG85950.1"
                     /translation="MNLDCIRHPRLREWIDENVAMCMPDDVVVCDGGHEEADRLAERM
                     LRSGSFKRLEKRPNSFAVRSDPGDVARVENNTFICSRFREEAGPTNNWRDPQEMKGEL
                     RQRFFGCMRGRTMYVIPFSMGPVGGPLSQIGVELTDSVYVVLNMRIMTRMGEAVLKKL
                     DRDKPFVKCLHSVGYPLTLGRHELPWPCDSNNKYIVHFPEERSIWSYGSGYGGNALLG
                     KKCYALRIASVMARDQGWLAEHMLILGITNPDGKKKYIAAAFPSACGKTNLAMLVPNL
                     PGWKVETVGDDIAWLRWGDDGRLYAINPEYGFFGVAPGTSLKSNPNAMRSTEKNCIFT
                     NVATTPDGDVWWEGIGGEPPAGLVDWLGEPYDPSKGKPAAHPNSRFTAPASQCPVIDQ
                     AWEDPRGVPISAILFGGRRAGTVPLVHEAMDWEHGVFMGSIVSSETTAAAAGAVGQLR
                     RDPFAMLPFCGYNMGDYFHHWLRMGERSGVKHPRFYYVNWFRKGADGKFLWPGFGDNA
                     RVLKWIFERCDGAGEAVETPIGNVPAPGALDLDGLDLSPEAMAELLTVREDEWLADVP
                     GIRAFFGSFHDGFPEELVARLDWLERRLRHPAAKRHRSAIA"
     gene            complement(3021..4241)
                     /locus_tag="PLQ29_04565"
     CDS             complement(3021..4241)
                     /locus_tag="PLQ29_04565"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPG85951.1"
                     /translation="MNPIDIYVNKSVPLKFKGQEMSFELSHALFSSFDIDAGSRLLLK
                     LVAKNVEDGLVGSILDVGSGVGVLGVACARGYPGASLRLRDRDALACAFSERNARRNK
                     VRGATVDRALFLDGLGSERFDLALCNVPAKAGPPVLDRFLRDLPGVLTDSGRGAVVVV
                     QTIAEAAAESIRGSGAEIVATERGPGHTALLFARGGAAAYADDGSSYAVERSERRGLR
                     AGKSVYAIKGYWGLPEFDTSSFATELAVELFEHASAGLLLRRAAVVNPGVGALASYVG
                     VRAKGATIDLCGRDSLALVASARNLALATRSGSEPGAAFAFQADLPDSAYDLLVEFPD
                     LTPRVDTTGDSWETAARVLKRGGAFVASMPSTAMDRFERRRPKGFTRLAEKKKKGFAC
                     AAWRFDGLEVSAEA"
     gene            complement(4313..4822)
                     /locus_tag="PLQ29_04570"
     CDS             complement(4313..4822)
                     /locus_tag="PLQ29_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPG85952.1"
                     /translation="MKESVFNQYLTFTLHDEHYAVSVGKVREVLEYTRITKLPRTADY
                     MKGIINLRGKGVPVIDLRLKFGMDETPVAKDTAIIVLDVEGDDGEVIVGALADSVHEV
                     VEIAGDRIEPAPRFGTKLAAEFIDGIGKRDDAFIVILNIDRIFNSDEVAMLRAQDAAA
                     VAETVDTAK"
     gene            complement(4846..>5341)
                     /locus_tag="PLQ29_04575"
     CDS             complement(4846..>5341)
                     /locus_tag="PLQ29_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HPG85953.1"
                     /translation="ETMEDSGAPSGCGSCRACADACPTGAIGPGGVDLDRCLQAWATR
                     PGTMPEAAARAWGSRLYGCDACVLACPRSARAYERRPGSDGGPDGGPSPAQAAEALLL
                     PAERRPGPFVSSRLVTGSGDAELSAFFRKTALGLSWIRGEELRRNAAIALGAGAFDAD
                     ADRR"
BASE COUNT          806 a         1829 c         1828 g          878 t
ORIGIN      
        1 gttcctggcg gccaagggca tagcgcccgg cctgccgccc atcgtcggcg cgctggtctt
       61 cgcggtgatc gccgcggcgg tgataggctt cgtgtcgtta cgcgccaagc agcgcgagga
      121 cacggtgatc aacgcgatct gggccattgg catgagcgtc ggcgtgctgt tcatcgccaa
      181 gacccccggc tacgccgacc cgatgagcta cctgttcggc aacatcctgc tcgtatccgg
      241 ccgagacctc gcgctgctcg ccgcgctcga cgcggtcgtg ctgttcctcg cctggcgctt
      301 ctatccgcag atagaggcga gcgccttcga cgaggaattc gccaaggtgc gcggcgtgcc
      361 gaccgacgcc gtcttcgtgg cgaccctcgc cgtcacggcc gttgcggtcg tgctgctcca
      421 gaccttcgtc ggcatcgtga tggtcatcgc gatgctgacc ctgccctcgg gcaccgccgg
      481 cttcgccgcc aggagcctgg cggcgatgat ggccgggggc gccgcgctct cggccgcctt
      541 cacggtcgct ggcctggccg ccagctggtc gctcgacctg ccggccggcg ccgtcgtcgt
      601 cgtgatagcc ggcgccgctt tcctcgcggt atcggcggcg gtcgccgtca aggccaggaa
      661 ggcccgcggg ggccgcgcgt gacccgggcc agggccgcgg tgctggccgc cctggagtcg
      721 accgtcgagc cgctatcggc ggctggcgta gcctcgcgga tggccggcgc ctgcgaccag
      781 gccacggtgt accgcgcgct ccatcacctc gaggaggcag ggctggcgga gtcgttcgtg
      841 ctgcactgcg cgatgcacgg cgtcgagcgc tactacgccg cgaccagctc gccccaccgg
      901 cactggttcc actgcgagag ctgccatcgc ttcgtcgacc tcggcgcgtg caggctgggc
      961 gggctcgtct ccgaactgga gcgcgagcgc ggcctacgcg tcacgaggca cacgatgtac
     1021 ctgaccggcg tctgcggcga ttgcgccgcc cgggagtgag gacggccccg ggccgagccc
     1081 tgaggccgtc ccggggctgg ggatactcag gctatcgccg agcggtgacg cttggcggcg
     1141 ggatggcgca ggcgccgctc cagccagtcg agccgagcga cgagctcctc cgggaacccg
     1201 tcgtggaagc taccgaagaa ggcgcggata cccggcacgt cggccagcca ttcgtcctcg
     1261 cgcaccgtca ggagctcggc catcgcctcg ggcgacaggt cgaggccgtc gaggtcgagg
     1321 gcgcccggcg cgggcacgtt gcctatcggc gtctccaccg cctcgccggc tccgtcgcag
     1381 cgctcgaaga tccacttgag cacgcgcgcg ttgtcgccga agcctggcca gaggaatttg
     1441 ccatcggctc ccttgcggaa ccagttgacg tagtagaacc tagggtgctt gacgccggag
     1501 cgctcgccca tcctgagcca gtggtggaaa tagtcgccca tgttatagcc gcagaacggc
     1561 agcatcgcga aggggtcgcg cctgagctgg ccgacggcgc cagcggcggc ggcggtggtc
     1621 tcgctcgaga ctatggaacc catgaacacg ccatgctccc agtccatcgc ctcatggacg
     1681 agcggtacgg tacccgccct gcgcccgccg aacaggatgg cgctgatcgg cactcccctg
     1741 ggatcctccc aggcctggtc gatcacgggg cactgggacg cgggggcggt gaagcggctg
     1801 ttcgggtggg cggccggctt gcccttggac gggtcgtagg gctcgccgag ccagtcgacg
     1861 aggccggccg gaggctcccc gcctatgccc tcccaccaga catcgccgtc cggcgtcgtg
     1921 gccacgttcg tgaagatgca attcttctcc gtggagcgca tcgcgttggg attggacttg
     1981 aggctggtgc ccggcgcgac gccgaagaag ccgtactccg ggttgatggc gtagagccgc
     2041 ccgtcgtcgc cccagcgcag ccaggcgatg tcgtctccga ccgtctccac cttccagccc
     2101 ggcaggttcg gcacgagcat ggccaggttg gtcttgccgc aggccgaagg gaaggccgcc
     2161 gcgatgtact ttttcttccc atcgggattc gtgatgccga ggatgagcat atgttcggcg
     2221 agccagccct gatcccgcgc catgacactg gcgatgcgta gcgcgtagca tttcttgccg
     2281 agcagcgcgt tgccgccgta gcccgagccg tacgaccaga tcgagcgttc ctcggggaag
     2341 tggacgatat acttattgtt cgagtcgcag ggccagggta gctcgtgccg gccgagcgtc
     2401 agcgggtagc ctaccgaatg caggcacttg acgaagggct tgtcgcggtc gagcttcttg
     2461 agcacggcct cgcccatgcg cgtcatgatg cgcatgttca gcacgacgta gacgctgtcg
     2521 gtcagttcga cgcctatctg ggacaggggg ccgcctacgg ggcccatcga aaacggaatg
     2581 acatacatgg tgcgaccgcg catgcacccg aagaagcgct gcctgagctc gcccttcatc
     2641 tcctggggat ctcgccagtt gttcgttggg ccggcctctt cgcggaaacg cgagcagatg
     2701 aaggtattgt tctcgacgcg ggcgacgtct cccgggtcgc tacggaccgc gaacgagttg
     2761 ggacgcttct ccagccgctt gaagcttccg gagcggagca tgcgctcggc cagcctatcg
     2821 gcctcctcat gcccgccgtc gcagacgacc acgtcgtccg gcatgcacat ggccacattc
     2881 tcgtctatcc actcgcggag gcgtggatgc ctgatgcaat cgaggttcat accgagggtc
     2941 tccttcttcg cggacagttt taaacccgaa acgcgtattg cacaagataa aacgatcgcg
     3001 gccggaacgg cgcctggacc ttaggcttct gccgagacct ccaggccgtc gaagcgccac
     3061 gcggcgcagg cgaagccctt cttcttcttc tcggccaggc gcgtgaagcc cttgggcctc
     3121 cgccgctcga aacggtccat cgccgtcgac ggcatcgagg cgacgaaggc cccgccgcgc
     3181 ttgagcaccc tggcggccgt ctcccacgag tcgccggtcg tgtcgacgcg cggcgtgagg
     3241 tcggggaact cgacgaggag atcataggcc gaatcgggca ggtcggcctg gaacgcgaag
     3301 gcggcgcccg gctcggaacc ggaccgggtg gccagcgcca ggttcctggc gctggcgacg
     3361 agcgccagcg agtcgcggcc gcacaggtct atcgtcgcgc ctttggcgcg gaccccgacg
     3421 taactggcca acgccccgac tcccggattg acgacggccg cccgcctgag cagaaggccg
     3481 gccgaggcgt gctcgaacag ctcgacggcg agctccgtgg cgaaggacga ggtgtcgaac
     3541 tcggggaggc cccagtagcc cttgatcgcg tagacggact tgccggcccg caggccccgt
     3601 cgttccgaac gctcgacggc gtacgacgac ccgtcgtctg catacgcggc ggcgcctccc
     3661 ctggcgaaca gcagggccgt atgcccgggc ccgcgctcgg tcgccacgat ctcggcccct
     3721 gaaccgcgga tggactcggc cgcggcctcg gctatcgtct gcacgacgac gacagccccg
     3781 cgccccgagt cggtcaggac gccgggaagg tcgcgcagga agcgatccag gaccggcggg
     3841 ccggccttgg ccggcacgtt gcacagcgcc aggtcgaagc gctcggaacc gagcccgtcg
     3901 aggaacaggg cccggtcgac cgtggcgccg cgaaccttgt tgcgcctggc gttgcgctcg
     3961 ctgaaggcgc aggcgagggc gtcgcggtcg cgcaggcgca gactggcgcc cgggtagccc
     4021 ctggcgcagg cgactcccag cacgccgacg ccggagccga cgtcgagtat cgagccgacg
     4081 aggccgtcct cgacgttctt ggccacgagc ttgaggagca gcctggagcc cgcgtctatg
     4141 tcgaaggagc tgaacagggc gtgggacagc tcgaagctca tctcctggcc cttaaatttg
     4201 agcgggacgc tcttgttgac gtatatgtcg atagggttca tctcggccat agtacgacgg
     4261 cccggccttc gtagcaagcg ccgggcgggc ccgccctgca cgggcgtata ggctacttcg
     4321 ccgtatcgac ggtctcggcg accgcagccg cgtcctgggc tcgcagcatg gccacctcgt
     4381 ccgagttgaa gatgcggtcg atgttcagga tgacgatgaa cgcgtcgtcg cgcttgccta
     4441 tgccgtctat gaactccgcc gcgagcttgg taccgaatcg gggagccggc tcgatacggt
     4501 ccccggctat ctcgaccacc tcgtggaccg agtccgccag ggcgccgact atcacctcgc
     4561 cgtcatcgcc ctcgacgtcg agcacgatga tggccgtatc cttggcgacg ggcgtctcgt
     4621 ccatgccgaa cttgagccgc aggtcgatca ccggcacgcc tttgccgcgc aggttgatga
     4681 tgcccttcat gtagtcggcg gtgcgcggca gtttggtgat acgggtatat tcgaggacct
     4741 cccgcacctt gccgaccgat acggcgtagt gctcgtcgtg taacgtgaag gtcaggtact
     4801 ggttgaagac actctctttc atcgctcggt actcctgccc taactctagc gcctgtccgc
     4861 gtccgcgtca aatgcgccgg ctcccagggc tatcgctgcg ttgcgcctaa gctcctcgcc
     4921 gcgtatccac gaaaggccga gcgcggtctt cctgaagaag gccgaaagct cggcgtcgcc
     4981 cgagccggtg acgaggcggg acgatacgaa gggtccgggc cggcgctcgg ccggcaagag
     5041 gagggcctcg gccgcctggg ccgggctcgg gcctccgtcc gggcctccgt ccgagcccgg
     5101 gcgccgttca taggcgcgcg ccgagcgtgg gcaagccagg acgcaggcat cgcacccgta
     5161 taacctgctt ccccaggcgc gcgccgccgc ctcgggcatg gttccgggcc gggtcgccca
     5221 ggcctgcaga cagcggtcca ggtcgacgcc gccgggacct atcgcgccgg tcgggcaggc
     5281 gtcggcgcag gcccggcagg atccgcagcc gctcggcgcg ccagagtctt ccatagtctc
     5341 t
//
LOCUS       DAOPHV010000122         5327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_20511_length_5327_cov_4.706753, whole genome shotgun sequence.
ACCESSION   DAOPHV010000122 DAOPHV010000000
VERSION     DAOPHV010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20511_length_5327_cov_4.706753"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PLQ29_04580"
     CDS             complement(<1..795)
                     /locus_tag="PLQ29_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG85954.1"
                     /translation="MKTGSNRYDVCVIGAGVCGANIARRLSAYELDVALVDKEADVSF
                     GTSKANSGIVHGGFHHNKKYLKARLELQGMMMFDRLQQELDFPFKRCGIVVAALHEDE
                     MRAIEQLYMQGVDNGVIGIEMCSRERILELEPKLNPDTLGGLWVPGGGIVEPYRFVFA
                     LVESARKNGVEIMTGFKVEHAARAGDEWTVRAADGRVVKARYVVNAAGLYADEVSRAF
                     GAEEFEIKPRKGEYYLLDRLTKAKPERVIFPVPTSVSKGMLVIPTVE"
     gene            907..2115
                     /locus_tag="PLQ29_04585"
     CDS             907..2115
                     /locus_tag="PLQ29_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HPG85955.1"
                     /translation="MGFIQRSCVRSIHGLLASHRLVLVEGPPRSGRSTIAAQVAAGAP
                     GGGILVDARRPEGRDVLADPASASAARLIVVDGAGRREAERVAASLSAFGEAERTPRF
                     ALIGGPFGHAAASRAATGRAPASRVAATLDAPILRVCPLSLFEAGSASMRRLWLRGGF
                     PQAFMADSDQAALAWLRDYAASLAGDSLSAWGVPRDAALVSGLLDALAASSGRAFNEN
                     AAARSLGVSRPTIERAVAAVERAGLLLRVPAWRPRDDIARDDGPPGTARAIRSPAIHL
                     ADSGLLHALHGAAPTLETAATAASWTSFVIGQALSVLRPGTAIFHYASADGAALELVV
                     AMDGRPVVAAAARRHRPSSAERAVSYAARAIGATERYIVVPEGGGAKLPGGFIVTGLR
                     AFLDRLAAVP"
     gene            2146..2862
                     /locus_tag="PLQ29_04590"
     CDS             2146..2862
                     /locus_tag="PLQ29_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG85956.1"
                     /translation="MLEVRGFVKRFGSRTVLDHLDAVVDEGRVTALVGPNGSGKSTLL
                     KALWGEAPADSGKLLWRGEPIDARSTAWKRQVGAVPDDDALIEGLSIGGHFALCGGLS
                     GLSPATVEDRSERLVRLFALEDAMATTRMADEASRGNRKRLACALALLGEPELLLMDE
                     PFSGLDAERAAALTSILRALASRGLTILLSCHDDGITRRVADRFLLLRDGKAVTGQIG
                     ELPRGPATVPDTEDLLPWLG"
     gene            2850..4064
                     /locus_tag="PLQ29_04595"
     CDS             2850..4064
                     /locus_tag="PLQ29_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85957.1"
                     /translation="MARLSRTRPTGLVRLRLALSSLRSADDELRSRDRPGSALRAVSA
                     YLVGFAIMRTATNAYLFLSTLGRLASPAEIRGSAAVLVALACAPLLSAACLSGDRTGA
                     ERRLALLPLSRPLRLTIAALSPAASAATIALLASILPAIAAAPFALRSIGLWLAVAAW
                     SSFTATAAGLGLRSAASAITAATRRSRPRDRGHGRRVALLAAMLASAAANPSPSMAAG
                     RLVVTLFGSVELAAADLANLAGRLSVDSMAALAAVAGSFALAAAIAEDAVLSGGRRAE
                     PRRRPARSPSSGRGASLPGGRLAYPLAMAAAAARGRRLGPALAASLAFSSLCVSQGAP
                     TAIPLTAAAAFLAVTLGSALASMATDSQTTRRFAMLGDGAAERGYLATSATLAMVTAA
                     PLCAAALAAAFR"
     gene            4145..4828
                     /locus_tag="PLQ29_04600"
     CDS             4145..4828
                     /locus_tag="PLQ29_04600"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HPG85958.1"
                     /translation="MAACTIGMNGSRISVAILAGGESRRMGRDKGLLPLGGKCMIEAV
                     AERLSPYADELIIGSNDAAAYAFTGFRVVPDLERGHGPMMGVLSCLEAARNGSVLFAP
                     CDVPVIPDAFVRLMLETAMDADIAMPLDELGRPEPLLAAYSRRTIPAIRRLLEGGVRR
                     MAALMGDDLRSEYGLTLSLARYPDMGWYRNVNTMADYESLLAELAGAPAVERAELAEA
                     PAMERAERP"
     gene            complement(4835..5218)
                     /locus_tag="PLQ29_04605"
     CDS             complement(4835..5218)
                     /locus_tag="PLQ29_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85959.1"
                     /translation="MILIEGRVVEGATRVLVAGGATGLEGLKAPLDGFDALVTAERGL
                     SDLAAASGLSVFSPGESGAFIERYLSGGSMESRDVTVTTGGVEVPINPFIKETIENVV
                     VGLVKPLKKTDIDGEIVIRIGPART"
BASE COUNT          750 a         1875 c         1853 g          849 t
ORIGIN      
        1 ctcgacggtc ggtatgacga gcatgccctt ggagacgctc gtcggcacgg ggaagatcac
       61 gcgctcgggc ttggccttgg tcagccggtc caggaggtag tactcgccct tgcgcggctt
      121 gatctcgaac tcctcggcgc cgaacgcgcg agacacctcg tcggcataga ggcccgcggc
      181 gttgaccacg tagcgcgcct tgacgacgcg gccatccgcc gcgcgtaccg tccactcgtc
      241 gccggcgcga gccgcgtgct cgaccttgaa gccggtcatg atctcgacgc cgttcttgcg
      301 cgcgctctcg acgagggcga acacgaagcg atacggctcg acgatgccgc cgccggggac
      361 ccatagtccg cccagggtgt cggggttgag cttgggctcc agttcgagga tgcgttctcg
      421 ggagcacatc tcgatgccga tgaccccgtt gtcgacgccc tgcatgtaca gctgctcgat
      481 ggcgcgcatc tcgtcctcgt gcagggccgc gacgacgatg ccgcaacgct tgaacgggaa
      541 gtcgagctcc tgctgcaacc tgtcgaacat catcatgccc tgcaactcga gccgtgcctt
      601 gaggtacttc ttgttgtgat ggaagccgcc gtgcacgatg cccgagttag ccttggacgt
      661 gccgaaggat acgtcggctt ccttgtcgac gagcgccacg tcaagctcgt aggccgacag
      721 gcgcctcgct atattggcgc cgcagacgcc ggcgccgatc acgcagacgt cgtagcgatt
      781 cgatccagtc ttcacccctg acccccttgc tacctatcag tataggacga aaatggaccc
      841 tagcaagatg aacgctcgat gctagccact tttattaccg cattttaaac gctatgcttt
      901 catacaatgg ggtttattca gcgttcctgt gtccgaagca tccatgggct gctcgccagc
      961 catcggctcg tgctcgtcga ggggccgccg cgctccggca ggtcgacgat agcggcgcag
     1021 gtggcggccg gcgctccggg cggcggcata ctcgtcgacg cgcgcaggcc ggaggggcgc
     1081 gacgtcctcg ccgatccggc gtccgcctcg gccgctcgac tgatcgtggt cgacggcgcc
     1141 gggcggcgcg aggccgagcg cgtagccgcc agcttaagcg ccttcggcga ggctgagcgt
     1201 acgccgcgct tcgctcttat aggcgggccg ttcggccatg cggccgcgag ccgggcagct
     1261 acaggtcggg cacctgcgag ccgcgtggcc gcgacgctcg acgcgcccat actccgcgtc
     1321 tgccccttga gcctgttcga ggcaggctcg gcctcgatgc ggcgcctatg gctacgaggc
     1381 ggctttccgc aagccttcat ggcggacagc gaccaggccg cgctcgcctg gctacgcgac
     1441 tacgccgcca gcctagccgg agactcgctg tcagcctggg gcgtaccgcg cgacgcggcc
     1501 ctcgtctcgg ggctgctcga cgccctggca gcctcgagcg ggcgcgcctt caacgagaac
     1561 gccgcggcca ggagcctcgg cgtttcaaga ccgacgatag aacgcgccgt ggcggccgtc
     1621 gagcgggccg ggctcctgct acgcgtgccg gcctggcggc cgcgagacga tattgcgcga
     1681 gacgacggtc cgccgggcac ggcgcgtgcc atccgatcgc cggcgatcca cctggccgac
     1741 tcggggctgc tccacgcact gcacggcgcc gccccgacgc tcgagactgc ggccacggcc
     1801 gcctcgtgga cgtccttcgt tatcgggcag gccctgagcg tccttaggcc tggaacggct
     1861 atcttccatt acgccagtgc ggacggcgcg gccctcgagc tggtcgtggc catggacggc
     1921 cggcccgtcg tcgcggcagc cgcgcgccgc catcgtccga gttccgcgga gcgggccgta
     1981 tcgtacgcgg cgcgggcgat cggcgccacg gagcgctaca tcgtcgtgcc cgagggaggc
     2041 ggcgcgaagt tgcccggtgg cttcatcgtc accggcctgc gggccttcct ggatcgactc
     2101 gcggccgtac cttgaccggc gtggagagcg gacgcacact aagccatgct cgaagtacgt
     2161 ggattcgtca agcgcttcgg tagccggacg gtgctcgacc acctcgacgc tgtcgtcgac
     2221 gagggccggg tgacggccct tgtcggcccg aacggctccg gcaagtcgac cctgctcaag
     2281 gcgctctggg gcgaggctcc ggccgactcg ggcaagctac tctggcgtgg cgagcctatc
     2341 gacgctcgct cgacggcttg gaagcgccaa gtgggcgcgg tacccgacga cgacgcgctg
     2401 atcgagggcc tgtcgatagg cggccacttc gcgctgtgcg gcggactgtc cggactgagc
     2461 ccggcgaccg tcgaggatcg gtctgaacgg ctcgtcaggc tgttcgcgct cgaggacgcc
     2521 atggcgacga ccagaatggc cgacgaggcc tctcgcggca accgtaagcg cctggcctgc
     2581 gcgcttgccc tgctcggcga gccggagcta ctcctcatgg acgagccgtt ctccgggctc
     2641 gacgcggaac gggccgccgc gctgacgtcc atcctccggg ccttggcgtc ccgaggattg
     2701 acgatcctgc tatcctgcca cgacgacggc atcacgcgga gagtagccga ccgcttcctg
     2761 ctcctgcggg acggcaaggc agtgaccggc cagataggcg agttgccgcg cggccccgcg
     2821 actgtcccgg acacggagga cctgctgccg tggctcggct gagccgcacc aggccgacgg
     2881 ggctcgtccg gctgcggcta gcgctgtcat cgctacgctc cgccgacgac gagctacgct
     2941 cgcgggaccg gcctggctcg gctctccggg ccgtgagcgc ctacctggta ggcttcgcga
     3001 tcatgaggac cgcgacgaac gcgtacctgt tcctgtctac gctcgggcgc ctggcgagcc
     3061 cggctgagat ccgcggttcg gcggccgtac tcgtcgcgct ggcctgcgcg cccctcctgt
     3121 cggccgcctg cctgtcgggc gaccgtaccg gagccgagcg caggctggcg ctcctgccgc
     3181 tatcgaggcc gctcaggctg accatagccg cgctttcacc cgcggcctcc gccgcgacga
     3241 tagccctgct cgcctcgatc ctgccggcca tagcggccgc gccgttcgca ttacgctcga
     3301 tcggcctctg gttggccgtc gcggcatggt catcgttcac ggcgacggcg gccgggctcg
     3361 gcctacgctc ggccgcctcg gcgataaccg ccgcgacgcg ccgatcgcgg ccgcgggacc
     3421 gcggccacgg acgccgcgta gcgctgctcg ccgcgatgct ggcctcggcg gcggccaacc
     3481 cgagcccgag catggcggcg ggccggctcg tcgtgacgct gttcggttcc gttgagctgg
     3541 ccgccgccga cctggcgaac ctcgccggcc gcctgagcgt agactcgatg gcggccctcg
     3601 ccgcggtggc cgggtccttc gcgctggcgg cggccatcgc cgaggacgcc gtactgagcg
     3661 gcgggcgccg tgccgaaccc cgacggagac cggccaggtc gccatcgtca ggacgcggcg
     3721 ctagcctgcc gggtggccgg ctagcctatc cgctggcgat ggcagccgcg gccgcccgtg
     3781 gacggcgctt gggcccggcc ctggccgcgt cgctagcctt ctcgtcgctg tgcgtctcgc
     3841 agggagcgcc gacggccatt ccgcttacgg cggccgcggc tttcctcgcc gtgacgctcg
     3901 gctcggcgct cgcctccatg gccaccgata gccagacgac gcgtcgcttc gccatgctcg
     3961 gcgacggcgc ggcggagcgg ggctacctgg cgacctcggc caccctcgcc atggttacgg
     4021 ccgccccgct ctgcgcggcg gccctggccg ccgcgttccg ctaatggtat atcgtcgcgg
     4081 cggccggaag gccgccgcca agccaagcca tgacgaagcg accgagggcc ggtatagtag
     4141 gcccatggca gcgtgtacca tcgggatgaa tgggtctagg atcagcgtcg cgatcctggc
     4201 tggcggggag agccggcgga tgggacggga taaggggctc ctcccgctcg gcgggaagtg
     4261 catgatcgag gcagtcgcgg agcggctgtc gccctacgcc gacgagctga tcatcggctc
     4321 caacgacgcg gctgcctacg cgttcaccgg cttccgcgtg gtgccggatc tggagcgcgg
     4381 ccacgggccg atgatgggcg tgctgtcctg ccttgaggcg gcgcggaacg gctcggtgct
     4441 gttcgcgccg tgcgacgtac cggtgatacc ggacgccttc gtacggctca tgctcgagac
     4501 ggccatggac gcggatatag cgatgccgct cgacgaactc ggccgaccgg aacccttgct
     4561 cgcggcgtac tcgaggcgga cgatcccagc gatcaggcgg ctcctggaag ggggcgtgcg
     4621 acgcatggcc gcgctgatgg gcgacgatct acgctcggag tacggcctga cgctatcgct
     4681 cgcgcgctac ccggacatgg gctggtaccg caacgtgaac actatggccg actacgagtc
     4741 gctactggcc gagctggcag gggcgcctgc cgtggaacgg gccgagctgg cagaggcgcc
     4801 agccatggaa cgggccgagc gaccgtagcg cccgctacgt ccgcgccggg ccgatgcgga
     4861 tgacaatctc gccgtcgata tcggtcttct tgagcggttt gacgaggccg acgacgacgt
     4921 tctctatcgt ctccttgatg aaggggttga tcggcacctc gacaccgccg gtcgtcacgg
     4981 tcacatccct gctttccatg cttcctcccg ataggtagcg ttctatgaag gccccggact
     5041 cgccggggga gaagactgac aggccggagg cggccgcgag atccgaaagg ccgcgctcgg
     5101 cggtaacgag ggcgtcgaag ccgtcgaggg gcgctttgag cccctcgagc cccgtcgcgc
     5161 cgccggcgac caggacccgc gtcgcgcctt cgacgactcg gccctcgatc aggatcacgt
     5221 cggcggaggg gaacagcgac tcgacccagg cgcggcccca atcgggaggc gtcctcgcgc
     5281 agggtgccca cgctcacccg tccgcccagc agttcgtaca ggccggg
//
LOCUS       DAOPHV010000123         5280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_20759_length_5280_cov_3.798278, whole genome shotgun sequence.
ACCESSION   DAOPHV010000123 DAOPHV010000000
VERSION     DAOPHV010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20759_length_5280_cov_3.798278"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLQ29_04610"
     CDS             <1..193
                     /locus_tag="PLQ29_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85960.1"
                     /translation="LTGRRLARLDRKVDAMATASLGPPAARAETPPAFLAIVRDEGRL
                     VEWPAERPSRARSPGYSPG"
     gene            complement(239..1366)
                     /locus_tag="PLQ29_04615"
     CDS             complement(239..1366)
                     /locus_tag="PLQ29_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HPG85961.1"
                     /translation="MYGGFMMEKPLGRVGGDPGMLVSLALLVGVGLACLYSASYGFAL
                     SLGKPGGYFAVRQLIWMAPAAAIFAFGAFLPLDAARRWIGVAVIGSLAMLVLPFVPGI
                     GITKNGASRWFGFGGQMFQPSELFKPVIVLYLAHILSKKGEKLGDVVNGVMPPLIVVM
                     AGALIVLAQNDFSTAALVITLAVVMFWISGVPLSFFAAIGSVGLPLVALSVLTSDYRL
                     KRVIGFVAPGYDPADLSYQVNGSLRAIRSGGFWGKGIGQGSLKLRSIPEVQSDFVFAA
                     WVEETGFAGVLAFAALWAVFLWRAYRVAFRSEDTFRSHLAFGIACYLGIQTLLNVMVA
                     SGAAPATGIPLPFFSSGGSSLVSAALSAGLLFNVSRSVNHG"
     gene            complement(1378..2460)
                     /gene="mraY"
                     /locus_tag="PLQ29_04620"
     CDS             complement(1378..2460)
                     /gene="mraY"
                     /locus_tag="PLQ29_04620"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPG85962.1"
                     /translation="MFLEWIYPLVKYFTPLNVFRYLTFRSAYSAVTALLVAFLFGPAI
                     IERLRRLKFGQSVRLDGPQSHLIKSGTPTMGGLLIIAAAVSATLLWVDLSNRYVWICL
                     GSTLGFGALGFLDDWLKIKRKNSDGVSAKAKLIGQAVIALCAALALYFDPSGEMTKLY
                     VPFFKAPVVDLGIFWIPLAVVYVMSWSNAVNLSDGLDGLATGLVIMALIAFSLLTYLT
                     GRADWSEYLGIPFVRGSGELTVFCLALLGACVGFLWFNAHPAEVFMGDVGSLSLGGVL
                     AVLALIVKKELLFFIIGGVFVLEEASVAIQVLYFKATKGKRVFRMAPLHHHFELSGWK
                     ETKVVTRFWILGGLFAIIALSTLKIQ"
     gene            complement(2450..3859)
                     /gene="murF"
                     /locus_tag="PLQ29_04625"
     CDS             complement(2450..3859)
                     /gene="murF"
                     /locus_tag="PLQ29_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPG85963.1"
                     /translation="MAEDRGACDAAWFAGAAGGYIASGSNASIGRVETDSRKAGPFAL
                     FVAIRGERSDGHDYARRAADAGSPAALVSASWWAIHGPAAFAGSPCAVIVVPDTLAAL
                     QKAAASYRARFPSLLRFGVTGSAGKTSAKELLAAIVGASRPTVKNPGNLNSDIGLPAS
                     MFLARPEHEAAVFEMGINRPGEMDELAAVYEPDCALITNVGTAHVGVLGGTRRAIAEQ
                     KKRIASRFDGRQTLIVWEDDDLRDTLMSGVRGRVETYGPRSTDGFEGARDLGLEGWAL
                     RYRGVEARLRLPGAHNLLNALAAIRAATLYGAGPEHVAAGLEAVSPLSGRTSVIRGEY
                     TVVDDSYNANAESAIAAIGFCDGANVTGGRIYVLGSMKELGAETVPSHRRVGEAVAGS
                     KADAVFFYGDEARDAYEAALAARPDGEYRWFGDYGELESAVAAAVTPGDLILVKASRS
                     MALERLVARLTGTGGDHVS"
     gene            complement(3852..4097)
                     /locus_tag="PLQ29_04630"
     CDS             complement(3852..4097)
                     /locus_tag="PLQ29_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85964.1"
                     /translation="MLILTAAQGARYYAVTVELRRLEAAQADWVEENRRLLADIAVAK
                     SRARVGASMAEAEGYRMVGPKTTLRIRVKPGAEKRDG"
     gene            complement(4254..5246)
                     /gene="rsmH"
                     /locus_tag="PLQ29_04635"
     CDS             complement(4254..5246)
                     /gene="rsmH"
                     /locus_tag="PLQ29_04635"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPG85965.1"
                     /translation="MSDQKPSFGHVPVMLNDALERLVPPSPDCLLIDGTLGLAGHSSA
                     FLSRYPGLRLIGVDADAEVQAIARARLEPYGDRVSLVNLFFDEFFSGYAAAVARGEAE
                     RPGLVLFDFGVSMFHFRESGRGFSLQGDERLDMRLSSGAERTAADLVAELDERELLRI
                     ISEYGEEPFAYHITKAIVRERAAARIDSTARLADIVRGAVPAKARYGRIHPATRTFQA
                     LRIAVNDELGRIERVLPAAISCLAEGGLIACISFHSLEDRIVKRTFRSLELRKERAYF
                     KEAGDRPIPVGEAERGLVILTKKPVEPADDEVAANPASRSAKLRVARAVGGARS"
BASE COUNT          964 a         1775 c         1768 g          773 t
ORIGIN      
        1 gctgactggc cgccggctgg cgcgcctgga ccgcaaggtc gacgcgatgg ccacggccag
       61 cctcgggccc ccggccgcgc gggccgagac gccaccggcg ttcctggcca tcgtgcgcga
      121 cgagggtcgg ctcgtggagt ggcccgcgga gcgaccgtcc cgagcgcgct cgcccgggta
      181 ctcgcccggg tagcctagcg ccctcggagg ggcgtaatcc ctgtccgcca tcgcaagctc
      241 agccatggtt tacgctcctc gaaacattaa atagaagccc ggcgctaagc gcggcggaca
      301 cgagcgaaga gccgcccgac gagaagaacg gtagcgggat acccgtagcc ggagccgcgc
      361 ccgaggccac catcacattg aggagcgtct gtatgcctaa atagcaagcg atcccgaagg
      421 ccaggtggct ccggaacgta tcctcggaac ggaatgcgac ccggtacgcc ctccagagga
      481 ataccgccca cagggcggcg aaggccagta cgcccgcgaa gccggtctcc tcgacccagg
      541 ccgcgaagac gaagtccgac tgcacctcgg gtatgctcct gagcttgagc gaaccctggc
      601 cgatgccctt gccccagaag cctcccgagc ggatggcgcg aagggagccg ttcacctggt
      661 acgacaggtc cgccgggtcg tagcccggcg ccacgaagcc gatcacgcgt ttgagcctgt
      721 agtcgctcgt cagcaccgac aaggcgacga gcgggaggcc gaccgagccg atggcggcga
      781 agaacgacag cggcaccccg gagatccaga acatgacgac ggccagggta atgacgagcg
      841 ccgcggtcga gaagtcattc tgggccagca cgatcagggc gccggccatc accacgatca
      901 gcggaggcat cacgccgttg acgacgtcgc cgagcttctc gcccttcttg gaaagaatat
      961 gggccaggta gagcacgatg accggcttga acagctccga tggttggaac atctgcccgc
     1021 cgaagccgaa ccagcgagag gcgccattct tggtaatgcc gatgccgggc acgaagggca
     1081 ggacgagcat ggccaggctg ccgatcacgg cgacgcctat ccagcgtcga gccgcgtcca
     1141 ggggcagaaa ggccccgaac gcgaagatgg cggcggccgg cgccatccag atcagctgcc
     1201 gtacagcgaa atacccgccc ggcttgccga gcgacagggc gaagccgtac gacgcggaat
     1261 agagacaggc caagccgacg ccgaccagca gcgcgagcga gacgagcatc ccggggtctc
     1321 ccccgacacg gccgaggggc ttctccatca tgaatccgcc gtacatggct ccgtctccta
     1381 ctgtatcttg agcgtcgaca gcgcgatgat cgcgaacagc ccgccgagta tccagaaccg
     1441 cgtgaccacc ttggtctcct tccagccgga cagctcgaag tggtggtgga gcggcgccat
     1501 gcggaagacc cgcttgccct tggtcgcctt gaagtacagc acctggatgg ctaccgaagc
     1561 ctcctcgagg acgaatacgc cgccgatgat gaagaacagc agctctttct tgacgatcag
     1621 ggccagcacg gccagcacgc ccccgaggct cagcgagccg acgtcgccca tgaatacctc
     1681 ggcagggtga gcattgaacc agaggaaacc gacgcaggcg ccgagcaggg ccaggcagaa
     1741 caccgtcagc tccccggagc ctcggacgaa gggtatgccc aggtactcgg accaatcggc
     1801 gcggccggtc aggtaggtca ggagcgaaaa ggcgatcagc gccatgatca cgaggccggt
     1861 cgccaggccg tccaagccat cggacaggtt gaccgcgttc gaccagctca tcacgtagac
     1921 cactgcgagc gggatccaga atatccccaa gtcgacgacg ggcgccttga agaacggcac
     1981 gtacagcttg gtcatttcgc cggacgggtc gaagtacagg gctaaggccg cgcagagcgc
     2041 tatgacggcc tggccgatca gcttggcctt ggccgagacg ccgtcggaat tcttgcgctt
     2101 gatcttgagc cagtcgtcca ggaagccgag cgccccgaag cccagcgtcg agccgaggca
     2161 gatccagacg taacggttcg acaggtcgac ccacaggagc gtcgccgaga ccgccgcggc
     2221 tatgatgagg aggccgccca tcgtcggcgt gccggacttg atcaggtggc tctgcggccc
     2281 atccaggcgg acggactggc cgaacttgag tcggcgcagg cgctcgatga tagccggccc
     2341 gaataggaag gctacgagca gagccgtcac ggccgagtag gcggaccgga aggtcaggta
     2401 gcggaatacg ttcaatggag taaaatactt gaccagcggg tagatccact caagaaacat
     2461 gatcgcctcc agtcccggtc aggcgggcga cgaggcgctc gagcgccatg gaccggcttg
     2521 ccttaacgag tatcaggtcg cccggggtta cagcggccgc gacggccgac tccagctcgc
     2581 catagtcgcc gaaccagcgg tactcgccgt ccggacgggc cgccagggcg gcctcgtagg
     2641 cgtcgcgagc ctcgtcgccg tagaagaaga cggcgtccgc cttggagccg gccaccgcct
     2701 cgccgacgcg gcggtgcgac gggacggtct cggctccgag ctccttcatg gagccgagca
     2761 cgtagatccg cccgccagta acgttcgccc catcgcagaa gccgatggcc gcgatggcgc
     2821 tctcggcgtt cgcgttatag gagtcgtcga cgacagtgta ctcgccgcgg atcacgctcg
     2881 tccggccgga cagcggcgag accgcctcca accccgcggc gacgtgctcg ggtccggcgc
     2941 cgtacagcgt cgcggcgcgg atcgccgcga gcgcattgag caggttatgg gcgccaggta
     3001 gcctgagcct ggcctcgacg ccgcggtagc gtagcgccca gccctcgagc cccagatcgc
     3061 gcgcgccctc gaagccgtcg gtcgagcggg gcccgtaggt ctcgacgcgg ccccggacgc
     3121 cggacatgag ggtgtcgcgg agatcgtcgt cctcccagac tatcagggtc tgcctgccgt
     3181 cgaagcggga ggctatgcgc ttcttctgct cggcgatggc ccttcgggta ccgccgagca
     3241 cgccgacgtg ggccgtgccg acgttcgtga tgagggcgca gtccggctcg tacaccgccg
     3301 ccagctcgtc catctcgccg ggccggttga tgcccatctc gaacaccgcg gcctcgtgct
     3361 ccggccgggc gaggaacatc gatgccggaa ggcctatgtc ggaatttagg ttgcccgggt
     3421 tcttgacggt ggggcgagac gcgccgacta tcgcggcgag cagctccttg gcgctggtct
     3481 tgccggccga gccggtgacg ccgaaacgca ggagcgaggg gaagcgcgcc cggtaggagg
     3541 cggccgcctt ctgcagggcg gcgagcgtgt ccgggacgac gatgaccgcg cagggagaac
     3601 ccgcgaacgc ggccgggccg tggatggccc accaggaagc cgagacgagc gcggctggcg
     3661 agcccgcgtc cgccgcccgg cgggcgtagt cgtggccgtc gctccgctcg ccgcgtatag
     3721 cgacgaagag cgcgaacgga ccggccttgc gggaatcggt ctcgacgcgc cctatggacg
     3781 cgttcgatcc gctcgcgatg tatccgccgg ccgcgcccgc gaaccaagcc gcgtcgcagg
     3841 ctcccctatc ctcagccatc ccgtttctcc gccccgggct taacccgtat cctcaacgtc
     3901 gtcttagggc cgaccatacg gtagccctcg gcctcggcca tcgacgcgcc caccctggcg
     3961 cgcgacttgg ccacggctat atcagcgagc aaccttctgt tttcctctac ccaatccgcc
     4021 tgcgccgcct ctaggcgccg caactcgacc gtcacggcgt agtacctcgc gccctgggcg
     4081 gccgtcagta tcagcatgca cggaatagac aggactaaga gcgtcagcca gactttcctc
     4141 accgtacgac cttccccggc ggggcacggg agggatgcag gctatggccg cgacgctcag
     4201 cgccactacg gccaccgctt ccatgatgaa ctcggccatg ccgatcgttc ctatcatgat
     4261 cgcgcccctc ctacggctct cgcgacccgg agcttggcgc tcctggacgc gggattggcc
     4321 gcaacctcgt catcagccgg ttccaccggc ttcttggtaa ggatgacgag gccgcgctcc
     4381 gcctctccta caggtatcgg ccgatcgccc gcctctttga agtaggcccg ttccttgcgt
     4441 agctccagcg agcggaaggt gcgcttgacg atgcggtcct cgagcgagtg aaagctgatg
     4501 caagcgatga gcccgccctc ggcgaggcag gagatggcgg ccggcaggac ccgctcgata
     4561 cggccgagct cgtcattgac cgcgatgcgt agagcctgaa aggtcctggt cgccggatgg
     4621 atgcgcccgt agcgggcctt ggccggcaca gcaccgcgta ctatgtcggc aaggcgcgcc
     4681 gtcgagtcta tcctggcggc ggcgcgctcc ctgacaatgg ccttcgtaat atggtaggcg
     4741 aagggttcct cgccgtactc ggagatgatc cgcaggagtt cccgctcgtc cagctccgcg
     4801 acgaggtcgg cggcggtccg ttcggcgccg gacgatagcc tcatatcgag cctctcgtcg
     4861 ccctggaggc tgaagccgcg cccggactcc ctgaagtgga acatcgagac gccgaagtcg
     4921 aacagcacga ggccaggccg ctcggcctcg ccccgagcta cggcggcggc gtacccggag
     4981 aagaactcgt cgaaaaacaa gttgaccaag ctaacccgat cgccgtaggg ctcgagcctg
     5041 gccctggcta tggcctggac ctcggcgtcg gcgtcgacgc ctatcaggcg tagccccggg
     5101 tagcgcgaca ggaaggcgct cgagtggccg gccaggccga gggtgccgtc tatcaggagg
     5161 caatccggcg acggcgggac gaggcgctcg agggcgtcgt tcagcatgac tggcacgtgt
     5221 ccgaaggacg gcttctggtc gctcataggt tctgtatgtc cagggtctcg gaggcggcgg
//
LOCUS       DAOPHV010000124         5207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_21170_length_5207_cov_3.875388, whole genome shotgun sequence.
ACCESSION   DAOPHV010000124 DAOPHV010000000
VERSION     DAOPHV010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21170_length_5207_cov_3.875388"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1301)
                     /gene="corA"
                     /locus_tag="PLQ29_04640"
     CDS             complement(201..1301)
                     /gene="corA"
                     /locus_tag="PLQ29_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPG85966.1"
                     /translation="MSGSRPARRRRIEPLFTSLADGREGSVPGSPVYIGDKTPTEAAF
                     SLIQYDKDELSIVTPENIEEVLAMLEEGAVNWININGLGDQDAIKRLGGYFKLDPLTV
                     EDILNTEHRPKLEDFGRYLFIITKTMSRRPDDSIEYEQISVVLTSNTVITIQESPGDC
                     YGPVRERLRTGAGRLRRMGASVLAYALLDVTVDHYFSLLEWLGTRLEEFEDTAAVASE
                     ATAFMSGLQDVKADLNRFKRILWPIRETVAELRHSESPLLDDSLEPFLRDLHDNSVQV
                     IEAIESYRETASGIQEVFLSSVSNRMNEVMKVLTIISTIFIPLTFIAGVYGMNFRHMP
                     ELDQPWAYPAAWGLMVAIAMGMITFFKKKKWF"
     gene            complement(1298..1681)
                     /locus_tag="PLQ29_04645"
     CDS             complement(1298..1681)
                     /locus_tag="PLQ29_04645"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPG85967.1"
                     /translation="MDEERRNEKRFRLSRMVAYDMEREQYIRAHGFEISKGGMAFLST
                     EYVEPDCVVWLSFSIPEEDGSWRQLEAEGRVREVSSIPSGTRFGVAFTRMADEDRVAL
                     DAFLDRLEREDGEPSDAGVPDGPSA"
     gene            complement(1775..4198)
                     /locus_tag="PLQ29_04650"
     CDS             complement(1775..4198)
                     /locus_tag="PLQ29_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPG85968.1"
                     /translation="MELNDEFIAGLEVGKADLLNKIASQLGIRASQAAAVVDLTAEGC
                     TVPFISRYRKERTGELDEVQVRDVVHLHQSLTNLETRRIEVIQAIHGQGKLTEELYGN
                     IRKCATLTEIEDIYAPYKRKKKTRGMIAVEKGLEPLAELMVSKDAAAVEAAAPGFVRE
                     DAEHPELAVASAEEAIKGAMDIVAERVSQDPENRAAVKKRYLRDGFMVVKGVGDDQKK
                     ESSTYQMYWDYRESLASLKPHRVLAINRGEREGELDVSLEVDEDDASALLKGRYAIAN
                     PYHADAIEDALRRLVSPAVLREIRSEAMDEADGHGISVFSENLKNLLLSAPLKGTRVL
                     GVDPGIRTGTKCAAIDETGKYLGYFVIYQEQKPDEARKALAKAIRDYRIELVAVGNGT
                     ASHEVQKLVADALGDLGGEAKYAVVDEDGASVYSASDVAREEFPELDLTIRGAISIGR
                     RIQDPLAELVKIDPKSIGVGLYQHDVNQKRLSETLDEVVESVVNRVGVNINTASHMLL
                     KYVSGINSSLAKKIVKYRDENGTITSRDQLKGVSGMGDKSFEQCAGFLKIPESPNPLD
                     NTWVHPENYALASELLEGVRAGKDPDKAQRDALKAKYGVGDTTIDDIVAELKKPNRDP
                     RDDYPKPVLQQGVLAFEDLREGMKVTGKVKNVVDFGAFVDIGIKESALIHVSELSDQF
                     VKDPMDVLKVGDVKEFRIISLDVARKRIGLSLKSERASGGQSFGNQRPSGYGGAQPGA
                     AQSPDSGTRRVVVAKKPAGGQSSPVGGQRSEKNPYSVGGRPGGRPSQDRDDDGTSYNP
                     FAELLKNRK"
     gene            complement(4213..4431)
                     /locus_tag="PLQ29_04655"
     CDS             complement(4213..4431)
                     /locus_tag="PLQ29_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85969.1"
                     /translation="MDEFRSLLEASELADRYRVSFFESTPENLARNADVQRLLSMASL
                     EPVICIGGKIAYLGGFSPEGLLAELRKR"
     gene            complement(4623..5120)
                     /locus_tag="PLQ29_04660"
     CDS             complement(4623..5120)
                     /locus_tag="PLQ29_04660"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPG85970.1"
                     /translation="MDDSRVRKASDLLGALLSPEAAAKADTWSKFFSFWNRAAGENLA
                     AHSRPVDLRNGIVFVEATHPGWIQLLQMRQTQILPTIRRSFPDLEVSGIAFRLAKDPS
                     LPGPAKMARPPEPIGAEGQRIDAVPEAASETPSVSETIGKVEDESFRSILSSLAETLG
                     GAGDE"
BASE COUNT          853 a         1636 c         1646 g         1072 t
ORIGIN      
        1 gcgacgctga gtttggggct atgaacgggt acggtgatgg cgctcgcata cgccgcgggc
       61 gagccttcgg ctagctctgc tgagacggtt cagccgcggg tacgagcagg acgggcccta
      121 tcgtagcgcg agcggcgtat accgtcaagc atcgcttttt acagtatata aatagaaaac
      181 taacgataat ctttaagaga ttagaaccac ttcttttttt taaagaatgt gatcattccc
      241 atagctatcg ccaccatgag gccccacgcc gcgggatacg cccacggctg atcgagttcg
      301 ggcatatgcc ggaaattcat gccgtacaca ccggcgatga aggtcaaggg aatgaagatc
      361 gtcgaaatga tcgtcaacac cttcatgacc tcgttcatgc ggttcgatac cgacgagagg
      421 aagacctcct ggatgcccga cgcggtctcc cgatagctct cgattgcctc tatcacctgc
      481 acggagttgt cgtgcaggtc gcggaggaac ggctcgaggc tgtcatccag gagcggcgac
      541 tcggaatggc ggagctcggc gacggtctcg cgaatcggcc ataggatgcg cttgaaccga
      601 ttcaggtccg ccttgacgtc ctgcaggccg ctcatgaaag cggtcgcctc gctcgccacc
      661 gcggcggtat cctcgaattc ctcgagccgc gtgccgagcc actcgaggag agagaaatag
      721 tgatccaccg tcacgtcgag gagggcgtag gccaggacgc tggcgcccat gcgccggagc
      781 cgccctgctc cggtccgcag gcgctcgcgt acgggcccgt agcagtcgcc agggctctcc
      841 tgtatcgtga tcacggtatt ggaggtcagg acgacggaaa tctgctcgta ctcgatgctg
      901 tcgtcgggac gccggctcat cgtcttggtg ataatgaaca ggtagcggcc gaaatcctcg
      961 agcttcggac ggtgttcggt gttgagtatg tcctcgacgg tcaagggatc gagcttgaag
     1021 tagccgccga gccgcttgat cgcgtcctgg tcgccgaggc cgttgatgtt gatccagttg
     1081 accgcgccct cctcgagcat ggccagcact tcctcgatgt tctcaggcgt gacgatcgac
     1141 agctcgtcct tgtcgtactg gatcagcgag aacgcggcct ctgtcggcgt cttatcgccg
     1201 atgtagaccg gactacctgg aacggaacct tcccgaccgt cggcgaggct tgtgaataga
     1261 ggttcaatgc gccgacggcg cgccggtctc gagccgctca tgccgagggg ccgtccggaa
     1321 cgcccgcgtc gctcggctcg ccatcctctc gttcgagccg gtcgaggaag gcgtcgagcg
     1381 cgaccctatc ctcgtcggcc attctggtaa aggctacgcc gaaacgagtc ccgctgggga
     1441 tgctgctaac ctcgcggacg cgcccttctg cctccagctg ccgccatgac ccgtcctcct
     1501 cgggaatgga gaacgatagc catacgacgc aatccggctc gacgtactcg gtcgacagaa
     1561 aggccatacc ccccttcgat atctcgaacc cgtgggcgcg gatatactgc tcccgctcca
     1621 tatcgtaggc gaccatccgt gagaggcgaa agcgcttttc attccgtcgc tcttcatcca
     1681 tgccataact atactaccgt tgtcggacga acgctataac aaacacgggc ccggccgagg
     1741 cacgttccgc gactgacccc tcgtcctcga ggagctattt cctgttcttc aggagctccg
     1801 cgaacggatt gtagctcgtg ccgtcgtcgt cccggtcctg tgacggacga cctccgggtc
     1861 tgccgcctac ggaatagggg tttttctccg acctctgtcc cccgacgggc gagctctgtc
     1921 ccccggccgg tttcttagcg acgacgacgc gccttgttcc ggaatcgggg ctctgcgccg
     1981 cgcccggctg cgcgccgccg taacccgatg gccgctggtt tccgaagctc tgtcccccgg
     2041 aggctcgttc gctcttgagc gacaggccga tgcgcttgcg ggcgacgtcg aggctgatga
     2101 tgcggaattc cttgacgtca ccgaccttga gcacgtccat aggatccttg acgaactgat
     2161 cggacagctc cgatacgtgg atcagggcgc tttccttgat gccgatgtcg acgaaggcgc
     2221 cgaagtcgac gacgttcttg accttgccgg tcaccttcat gccttcgcgg aggtcttcga
     2281 aggccaggac gccctgctgg agcaccggct tcggatagtc gtctcgcggg tcgcgattcg
     2341 gtttcttgag ctcggcgacg atatcgtcga tcgtcgtgtc gccgacgccg tacttggcct
     2401 tgagcgcgtc ccgctgggcc ttgtccgggt ccttgcccgc gcgcacgccc tcgagcagct
     2461 cggaggcgag ggcgtagttc tcggggtgca cccaggtatt gtcgagcggg ttcgggctct
     2521 cggggatctt gaggaagcct gcgcactgct caaagctctt gtcgcccatg cctgagacgc
     2581 ccttgagctg gtcccggctc gtgatcgtgc cgttttcgtc gcggtacttg actatcttct
     2641 tggcgaggga cgagttgatg cccgacacgt acttgaggag catatggctg gcggtattga
     2701 tgttaacgcc gacccggttg accacggact cgaccacctc gtcgagggtc tcggaaaggc
     2761 gcttctggtt gacgtcgtgc tggtagaggc cgacgccgat gctcttgggg tcgatcttga
     2821 cgagctcggc gagcgggtcc tggatgcggc ggcctatcga tatggcgcca cgtatggtca
     2881 ggtcgagctc ggggaactcc tcgcgagcca cgtcgctcgc ggaatagacc gaagcgccgt
     2941 cctcatcgac gaccgcgtac ttggcctcgc cgcccaggtc gccgagggcg tcggccacga
     3001 gcttctgcac ctcgtggctc gcggtgccgt tgccgacggc cacgagctct atcctatagt
     3061 cgcggatggc tttggcgagc gccttacgcg cctcgtcggg tttctgctcc tggtagatga
     3121 cgaagtagcc caggtacttg cccgtctcgt cgatcgctgc gcacttggta ccggtgcgta
     3181 tgccagggtc gacgccgagc acgcgcgtgc ccttgagcgg cgcggacagg agcaggttct
     3241 tgaggttctc gctgaacacc gagatgccgt gcccgtcggc ctcgtccatc gcctcggagc
     3301 ggatctcgcg taacacggct ggggagacga gccgcctgag cgcgtcttcg atcgcgtcgg
     3361 catggtacgg attcgcgatc gcgtagcgcc ccttgagcag cgccgaggcg tcgtcctcgt
     3421 cgacttcgag gctcacgtcc agctcgccct cgcgctcgcc gcggttgatc gccagcacgc
     3481 ggtgtggctt gagcgaggcg agcgactcgc ggtagtccca gtacatctga taggtcgagg
     3541 attccttctt ctggtcgtcg ccgacgccct tgacgaccat gaagccgtcg cgcaggtagc
     3601 gcttcttgac ggccgcgcga ttctcgggat cctgcgagac gcgctccgcg acgatatcca
     3661 tcgcgccctt gatcgcctcc tcggcgctcg cgacagccag ctcgggatgc tcggcgtcct
     3721 cacggacgaa accgggcgcg gcggcttcta ccgcggccgc gtccttggat accatcagct
     3781 cggcgagcgg ttcgaggccc ttctcgaccg cgatcatgcc gcgagtcttc ttcttgcgct
     3841 tgtacggcgc gtagatgtcc tcgatctcgg tcagcgtggc gcacttgcgg atattgccgt
     3901 agagttcttc ggtcagcttg ccctggccgt ggatggcctg gatcacctcg atgcgcctgg
     3961 tctccaggtt ggtcaggctc tggtgcaggt gcaccacatc gcggacctgc acctcgtcga
     4021 gctcgccggt gcgctccttg cggtagcggc tgatgaatgg cacggtacag ccctcggcgg
     4081 tcagatcgac gaccgcggcc gcctgactgg cccgtatgcc gagctgggag gctatcttgt
     4141 tgagcaggtc ggccttgccg acctcgaggc cggctatgaa ttcgtcgttc agttccatgc
     4201 tggtacctta ccttatcgct tgcgtagttc agcgagcaga ccttcgggac tgaagccgcc
     4261 gaggtaggct atcttgccgc cgatgcagat gaccggctcg aggctggcca tggacaggag
     4321 gcgctgcacg tcggcattgc gcgctaggtt ctccggcgtc gactcgaaaa agctcaccct
     4381 gtaccgatcg gccaactcgg acgcctcgag gaggctccgg aattcatcca ccagggcgag
     4441 ggtcgccctc ttcgcgcccg gcgcgcaact ggagcacgcg ccatcggccg agccctcgct
     4501 tccgtgagaa caaccggcgc agatgagctc tgacgctttg ccgtatacgt ctatatcgtg
     4561 tgtttggttc ataggcttcg tagaatcgtc acgatacggg gaactcctat aaaaatcaag
     4621 cgctattcgt ccccggctcc gcccagcgtc tccgcgagcg acgacagtat gctcctgaac
     4681 gactcgtcct cgaccttacc gatggtctcg ctgacactcg gcgtctccga cgccgcctcg
     4741 gggactgcgt cgatcctctg tccctcggcg cctatcggct cgggtggcct ggccatcttg
     4801 gcggggcctg gcaggctcgg gtccttggcc agcctgaagg ctatgccgga gacctcgagg
     4861 tccgggaagc tacggcgtat cgtcgggagg atctgggtct gcctcatctg gaggagctgg
     4921 atccatcccg ggtgggtggc ctcgacgaag acgatgccgt tccggaggtc gaccgggcgc
     4981 gagtgggcgg cgaggttctc tcctgcggcg cggttccaga acgagaagaa cttggaccag
     5041 gtgtcggctt tggcggccgc ctcgggggac aggagggcgc cgagcaggtc ggaggcttta
     5101 cgtacgcgag aatcgtccat cgtccgtcca gtatacgata gtgccatcgc gcaggtaatc
     5161 ccggtatggc tccccgggta agaaggtaaa gaacgactgc tctgctt
//
LOCUS       DAOPHV010000125         5205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_21182_length_5205_cov_4.677087, whole genome shotgun sequence.
ACCESSION   DAOPHV010000125 DAOPHV010000000
VERSION     DAOPHV010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21182_length_5205_cov_4.677087"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..849)
                     /locus_tag="PLQ29_04665"
     CDS             complement(4..849)
                     /locus_tag="PLQ29_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPG85971.1"
                     /translation="MNELRGLLQGLDGSLAGADDLSPSERTQLALCRSAIAFVLEGRL
                     AGEASANAPGTTAMKGAEGAGRIVIVEDDERLRRALVAELEPGSACEGYADAESALAG
                     LARDPAVAGFLVDFRLPGMDGLEFIREAFRRFPLGGTPAIVMTSLDDPALEERAYGAG
                     SVNFVRKPCEPRALRLRIEAAIASAHDGEERARRYLKARISRALEDEGAAASPLDEGA
                     ASRYGLSPRELDVARLLAGGLQAKEIADGLGLSIHTVMNHMRNGYAKCGVGNRIEFIR
                     RLSSP"
     gene            complement(846..2555)
                     /locus_tag="PLQ29_04670"
     CDS             complement(846..2555)
                     /locus_tag="PLQ29_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HPG85972.1"
                     /translation="MQYATPQLRSIDSLVRQGRFLQARSDALAYAAETGDGADRQARG
                     QLAFVLGKIYRNIGDYGDAEREYLSAAETFRVAGDDESTADALSGAALARWQLCDFAR
                     ATELQLEALRTYERIGDRLGLANAHNNLGLIAWKREDFSRAIESFEAALALYEELSHA
                     QGLAATVNNLGGVVQDAGDLERSAGYYGEAERLYRRAEDEAGAALAAGNLAWTLARLG
                     KPGEAAPFAERAVSGFRRAGDKAGLYRALMQRAAVLAAEGKPRDAAIDLYRAAHGARD
                     LGCAELESVTLQDLVEVLGSLGRHRRAAAWYGRILSLEKRIRDEASEREIARLRLDLE
                     LEFRKEEEERLKAALRDRDRLLSTLVHDARAATSRLRGSIASLRFELPSLDPGGLRSR
                     LGAVEEQAARLEAGLDSALAWARARDGSMQPAVEALPVLPALRSALAEVRQAMADKGM
                     ACGLDCPDPGPSVLADRRILGIVLGNALSNAVEHGAGPLAVSVRREGSVVSVDLRNAV
                     PGRPSLEASDASGASIRGLGLPLCAELCTRMGGSFSFVAKDGLALARVGLPCVEGRLS
                     STS"
     gene            complement(2587..4515)
                     /locus_tag="PLQ29_04675"
     CDS             complement(2587..4515)
                     /locus_tag="PLQ29_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPG85973.1"
                     /translation="MNPILRPRLCSPWGERLDPSSPLPEYPRPQLARPDWLSLNGPWD
                     YAIRPSGTCALADEVAPAFVAGDRDGSIVVPFAVETALSGVGKGLRPDETLWYRRTIR
                     VPEDWASRRIMARFEAVDYVAAVFVDGRLVGEHRGGYLPFAFELPPAGPGDVAAEREI
                     VVAVRDPSDAGLQQRGKQALKPAGILYTATSGIWQTVWLEPLPAGNAILGFRADATAG
                     LDGVTAVVDAERPAEVVLRASLPDGGVIEARGRSGEPIAVSVPDVRRWSPDDPYLYGV
                     EVELLGPDGDIVDRADSYFAIRRVDLGSAHGSPSGVPSGSPSGSRADERPFVRLNGEP
                     ILIHAPLDQGYWPESGMTPPADEALVFDIEAMRALGFNGLRKHVKVESRRFYWHCDRL
                     GTLVIQDAVSGGVNRSAGNVKVALPMIFGIHLGDRSGRALRLAGRSDPDNRAEFESDL
                     AGMMDLLRCHPSVVMWTVFNESWGQFESERVVEAVRRRDPTRLVDAVSGWHDQGGGDF
                     RSRHTYLVKLRRPPRRDRRPYFVSEYGGYNLAVPGHMWDDEARFGYRFYDDGPALQEG
                     YARLIRDELIPLVRSGLRVAVYTQVSDVEIESNGFYTYDRKVLKYDAATVRALNEELY
                     AASATVEAVRYEPRFPLA"
     gene            4596..>5205
                     /locus_tag="PLQ29_04680"
     CDS             4596..>5205
                     /locus_tag="PLQ29_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HPG85974.1"
                     /translation="MKHERLIAILDCLLNEGKKTAAELAGRFEVSERTIYRDIDSLCM
                     AGVPVVAEPGSGGGFEMDPSYRVDRSFLTRDEIADLSTLLRGMPEALKDGHIERSLGK
                     LSSLGAGAADRARRDEANGLPPPFIATLSPWGSPGPDPMTVLSLRGAIADRALVSFQY
                     VDGEGKPSERVVEPFSIVFGGGVWYLHGFCRLRSAWRIFKLSR"
BASE COUNT          752 a         1785 c         1857 g          811 t
ORIGIN      
        1 ggcctagggg gacgataggc ggcgtatgaa ttctatgcgg ttcccgacgc cgcatttggc
       61 gtagccgttg cgcatatggt tcatgaccgt atggatcgac agcccgaggc cgtcggctat
      121 ctccttggcc tggagcccgc cggcgaggag cctcgcgacg tcgagctcgc gggggctcag
      181 cccgtagcgc gaggccgcgc cctcgtctag gggcgaggcg gcggcgccct cgtcctcgag
      241 cgccctcgat atcctggcct tcaggtagcg cctggcgcgt tcctcgccgt catgggcgct
      301 cgcgatcgcg gcctcgatgc gtagcctcag cgcgcggggc tcgcagggct tccggacgaa
      361 gttgacgctt ccggccccgt aggccctctc ctcgagcgcc gggtcgtcca gggaggtcat
      421 cacgatcgcg ggggtcccgc cgagcgggaa gcggcggaag gcctcgcgga tgaattccag
      481 gccgtccatg cctggtagcc tgaagtccac caggaagccg gcgacggccg ggtctcgggc
      541 caggccggct agcgccgact cggcgtcggc gtagccctcg cacgcgctgc cgggctcgag
      601 ctccgcgacg agggcccgcc ggagccgctc gtcgtcctcg acgatgacta tccggcccgc
      661 gccctcggcg cccttcatcg cggtcgtgcc cggggcgttc gcgctcgcct cgcccgcgag
      721 ccggccctcg agcacgaagg ctatggcgct gcggcataag gccagctgcg tcctctcgga
      781 cgggctcagg tcgtcggccc cggctagcga gccgtcgagt ccctggagta gtccgcgtag
      841 ctcgttcacg aggtcgatga tagcctccct tcgacgcaag gcagtccaac gcgagccaag
      901 gccaggccgt ctttagctac gaagctgaag ctgccgccca tcctggtgca gagctcggcg
      961 cagagaggca gtcccaggcc cctgatcgag gcgccggacg cgtcgctggc ctcgaggctc
     1021 ggccgccccg gtacggcgtt gcgcaggtcg accgatacga ccgatccttc ccgccggacg
     1081 ctcacggcta gcggcccggc gccgtgctcg accgcgttcg atagcgcgtt ccccagtact
     1141 atccccagta tgcgccgatc cgccaggacc gacggcccgg ggtcggggca gtcgagcccg
     1201 caggccatgc ctttgtcggc catcgcctgg cggacctcgg cgagcgcgga ccgaagcgcg
     1261 ggtagcacgg gcagggcctc gacggcgggt tgcatggaac cgtcgcgggc ccgggcccac
     1321 gccagggccg agtccaagcc ggcctcgagc ctcgccgcct gttcctcgac ggcgccgagc
     1381 ctgctcctca gtccgccagg gtcgagggac ggaagctcga agcgaaggct cgcgatcgag
     1441 ccgcggagcc tggacgtcgc cgccctcgcg tcgtgaacca gcgtcgacag cagcctatcc
     1501 ctgtcgcgga gcgcggcctt gagccgttcc tcttcttcct tcctgaattc tagctccagg
     1561 tcgagcctga gcctggctat ctctcgctcg ctcgcctcat ctcgtatccg tttctcgagg
     1621 gacaggattc gtccgtacca ggccgccgcg cgcctatggc gcccgagcga gcccaggacc
     1681 tcgacgaggt cctggagcgt taccgactcg agctcagcgc agcctaggtc cctggccccg
     1741 tgggcggccc tatataggtc gatggcggcg tcgcgcggct tgccctcggc cgcgagtacc
     1801 gccgcacgtt gcatgagggc ccggtagagg ccggccttgt cgcccgcccg cctgaagccc
     1861 gataccgcgc gctcggcgaa gggggcggct tctccgggct tgccgagccg cgccagggtc
     1921 cacgccaggt tgccggctgc gagggccgcc cccgcctcgt cctcggcgcg cctatagagc
     1981 cgctcggcct cgccgtagta gccggcgcta cgttccaggt cgcctgcgtc ctggacgact
     2041 cctccgaggt tgttgacggt ggcggccagg ccttgggcgt gggatagctc ctcgtagagc
     2101 gcgagggccg cctcgaagct ctctatggca cgggagaagt cctcccgctt ccaggcgatg
     2161 agaccgaggt tattgtgggc gttggccagt cctagccgat cgccgatgcg ttcgtaggtc
     2221 cggagcgcct cgagctgcag ttcggtggcg cgggcgaagt cgcatagctg ccagcgcgcg
     2281 agggccgcgc cgctcagcgc gtcggccgtg gattcgtcgt cgcccgcgac acggaaggtc
     2341 tcggccgccg acaggtactc ccgctcggcg tcgccgtagt cgccgatgtt acgatatatc
     2401 ttgccgagca cgaaggccag ctggccccgg gcctggcggt ccgcgccgtc gcccgtctcg
     2461 gcggcgtacg ccaaggcgtc cgagcgcgcc tgaaggaagc gtccttgccg aacgagcgag
     2521 tcgatggaac gtagttgtgg cgtcgcgtat tgcatggatc ggcaccccgg ccttcgcaac
     2581 agtatatcac gcgaggggga aacgcggttc atatcgcacg gcctccaccg tcgcactcgc
     2641 cgcgtacagc tcctcgttga gcgcgcggac cgtcgcggcg tcgtacttga ggaccttgcg
     2701 gtcgtaggta tagaagccgt tgctctcgat ctcgacgtcg gagacctggg tgtagaccgc
     2761 gacgcggagg ccgctacgga cgagcggtat gagctcgtcg cggatcaggc gcgcgtagcc
     2821 ctcctggagc gcggggccgt cgtcgtagaa gcggtagccg aagcgcgcct cgtcgtccca
     2881 catgtggccg ggcacggcga ggttgtagcc gccgtactcg gagacgaagt acggccgccg
     2941 gtcgcgcctc ggcggtcggc gcagcttgac caggtaggta tggcggctac ggaagtcgcc
     3001 gccgccctgg tcgtgccagc cggagacggc gtcgacgagg cgggtcgggt cccggcgccg
     3061 tacggcctcg acgacgcgct cagactcgaa ctggccccat gactcgttga agacggtcca
     3121 catgacgact gatgggtggc agcgtagtag gtccatcatg ccggccaggt cggattcgaa
     3181 ctcggcacgg ttatcggggt cggagcggcc agcgagccgc agggcgcggc cggatcggtc
     3241 gccgaggtgg atcccgaata tcatcggcag ggctaccttg acgttcccgg ccgatcggtt
     3301 gacgcccccg gatacggcgt cctggatgac gagcgtgccg aggcggtcgc agtgccagta
     3361 gaagcggcgc gactcgacct tgacgtgctt gcggaggccg ttgaagccga gcgcccgcat
     3421 ggcctcgata tcgaagacga gtgcctcgtc ggccggcggg gtcatgcccg actccggcca
     3481 gtagccctgg tcgagcggcg cgtggatcag gatgggctcg ccgttcaggc gcacgaaggg
     3541 gcgctcgtcg gctcgcgatc ccgaagggga gcccgaaggg acgcccgaag gggagccgtg
     3601 cgccgagccg aggtcgacgc ggcgtatcgc gaagtacgag tcggcgcggt cgactatatc
     3661 gccgtccggc ccgagcagct cgacctccac gccgtacagg tacggatcgt cgggagacca
     3721 gcggcgcacg tccgggacgg atacggcgat aggttcgccg gatcggccgc gcgcctcgat
     3781 gacgccgccg tccggcaggg acgcgcggag aacgacctcg gccggccgct ccgcgtctac
     3841 gacggccgtg acgccgtcga ggcccgccgt agcatcggcg cggaagccga gtatggcgtt
     3901 gcccgcgggg agcggctcga gccagacggt ctgccagatg ccgctggtcg ccgtatagag
     3961 tatgccggcg ggcttgagcg cctgcttgcc gcgctgctgt agccccgcgt cgctcgggtc
     4021 gcgcaccgcg acgactatct cgcgctccgc ggcgacgtcg cccggtccgg cgggcggcag
     4081 ctcgaacgcg aacggcaggt agccgccgcg gtgctcgcct acgaggcggc cgtcgacgaa
     4141 cactgccgcg acgtagtcga ccgcctcgaa gcgcgccatg atgcgtcggc tcgcccagtc
     4201 ctcgggcacg cgtatcgtcc tgcggtacca gagcgtctcg tcgggacgta ggcccttgcc
     4261 gacgccggac agcgccgtct cgacggcgaa cggcacgacg atactgccgt cgcgatcgcc
     4321 ggccacgaag gcgggcgcga cctcgtcggc cagcgcgcac gtaccggacg ggcgtatcgc
     4381 gtagtcccac gggccgttga gcgacagcca gtccggcctg gcgagctgcg gcctcggata
     4441 ctcgggcagg ggcgacgacg ggtcgaggcg ctcgccccac ggggagcaga ggcgcgggcg
     4501 taggataggg ttcacggcgg ctccatgcgt atcgttttac gtgatagtat tagcgcttca
     4561 cgaactagtc aacgcgacga cgagagggaa ccggaatgaa gcatgagcgg ctcatcgcca
     4621 tcctcgactg cctgctcaac gagggcaaga agaccgcggc cgagctagcc gggcgcttcg
     4681 aggtgtcgga gcggaccata taccgcgaca tcgactcgct gtgcatggcc ggcgtgccgg
     4741 tagtcgccga accgggctcg ggcggcggtt tcgagatgga ccctagctac cgcgtcgaca
     4801 ggagcttcct gacgagggac gagatcgcgg acctgtctac cctgctgcgc ggcatgcccg
     4861 aggcgctgaa ggacggccat atcgaacgct cgctgggcaa gctgtcgtcg ctcggggccg
     4921 gagcggccga ccgcgctcgc cgcgacgagg cgaacgggct accgccgccg ttcatagcca
     4981 cgctgtcgcc gtggggctcg cccggccccg atcccatgac cgtgctgagc ctgcgcggag
     5041 ccatagccga ccgcgccctc gtgtcgttcc agtacgtcga cggggagggt aagccgagcg
     5101 agcgcgtcgt cgagccgttc tcgatcgtct tcggcggagg ggtctggtac ctacacgggt
     5161 tctgccggct ccgttccgcc tggcgtatat tcaagctgtc gcgcg
//
LOCUS       DAOPHV010000126         5179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_21327_length_5179_cov_3.806596, whole genome shotgun sequence.
ACCESSION   DAOPHV010000126 DAOPHV010000000
VERSION     DAOPHV010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21327_length_5179_cov_3.806596"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..2299)
                     /locus_tag="PLQ29_04685"
     CDS             complement(5..2299)
                     /locus_tag="PLQ29_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPG85975.1"
                     /translation="MIDEKKLGAIVAAAAAEGRDALTEPEGVEILGLMGLRVPVSLAV
                     RGSSEVARLASVPGDRVVVKVVSPEILHKTEAGGVAMVDNEPEAIRAAIADMEGRFGS
                     YRVDGYTINEYVRFEPRLGHEIIVGYRFARDFGPLVSFGPGGIYTEFLAKSFKPGAAT
                     LFLSPDGASRDSVRAALRGNAVRELLCGGLRGTKATIDEERLVDLVWAFVEAAPAFAR
                     AGITELEVNPFVVVRGADGSGSVVALDALAKLGRIAGDELSLAPDGSVVNRAQASRPV
                     DKIDRLLRPASAAVIGVSEKGMNNGRIILRNLIDNGFDRSRLYVVKPGCAEVDGVRCV
                     PDVASLPERVDLFVLSIPAAQTPGCIAELAERDKAEAVIVIPGGLEEKSGTEAIVGRM
                     RDALAASRGTKGRGPILNGGNCLGIRSVPGRYNTLFIPEYKLPMPSGQVDPVAFISQS
                     GAFAISRVSKHPDVNPKYLVTCGNQMDVTVGDYLERLADDEAIMLYAVYVEGFKPLDG
                     IKTLRAVRRIAASGRRVVFYRAGRTAAGAAASASHTASIAGDYPVTRALLRQAGAAVV
                     ESLDDFDDALTLFTAFVGKRPGPRLGAVSNAGFECVAMADNLGAMTLAELSAGTNERL
                     AKVIEAAKVGEIVDIHNPLDITPTGDDDAYVRSFEAIIDDGGVDVGLVGLVPLSGNIN
                     TLAAGSAHGEDVSRPGALPERLAELFRRSAKPWVVVVDSGELYDPLARELSRRGVPTF
                     RTADRALRMLNLWLEAAASPQTRA"
     assembly_gap    2792..2891
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3507..4859)
                     /locus_tag="PLQ29_04690"
     CDS             complement(3507..4859)
                     /locus_tag="PLQ29_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HPG85976.1"
                     /translation="MKLSKYSGDLSGIDYLSLLVIDINGAVRSVSLPRAYVSDKVLKG
                     GIGFDASNFGYAQVHKSDMVAVPDLSTGFVEEKDGFRILHAFCDVWTTDGEPFDQYPR
                     TVARKAMDALRSSGVGDDAKMLVELEFYAFDDARYSTTAGHSYYYVESAEGIGDDYAD
                     APRFGISKGYHRLAPDDRYGQLRNEAVRAMEAAGIPVKYHHHEVAASQLEIELNFISL
                     ASAGDAVVLAKWIVRSVAEELGLFVTFMPKPMYKVAGSGMHVHQYVEKDGKSVFPGDG
                     LYGLSGTGLAYTAGVLSHALSGSLLAWSNPSTNSYRRLVPGYEAPVSATFAQGSRAAA
                     VRIPGYLKKGEARIEFRTGDATANTYYFLAAMLLAGLDGVARGLDPVKLGYAVTKETP
                     KNTFPTGLFHVLSGLKKDCAYLQAAFPMELIDSWIAMKRQEAEYVYNAPVPQEYELYF
                     "
BASE COUNT          880 a         1702 c         1540 g          957 t
ORIGIN      
        1 acgttcaggc ccgcgtctgg gggctggcag ccgcctcgag ccagaggttc agcatccgca
       61 gggccctgtc ggcggtcctg aacgtgggga cgccacggcg ggacagctcg cgcgcgagcg
      121 ggtcgtacag ctcgcccgag tcgaccacca ccacccaggg cttcgcgctc cgcctgaaca
      181 gctccgcgag ccgctccggt agcgcgcccg gacggctcac atcctcgccg tgagccgagc
      241 cggctgccag cgtgttgatg ttaccggaca gcggcacgag gcccacgagg cccacgtcga
      301 cgccgccgtc gtcgatgatc gcctcgaagg accggacgta cgcgtcgtcg tcgccggtcg
      361 gcgtgatgtc gagaggattg tggatatcga cgatctcacc gaccttggcc gcctcaatca
      421 ccttggccag gcgctcgttc gtacccgcgg acagctcggc cagcgtcatg gctcccaggt
      481 tgtcggccat cgccacgcac tcgaagccgg cgttcgacac ggcgccgagc cttgggccgg
      541 gccgcttccc gacgaaggcg gtgaacagag tcagcgcgtc gtcgaagtcg tccaggctct
      601 cgaccacggc cgcgccggcc tgcctgagca gagcccgcgt caccgggtag tcgccggcta
      661 tgctcgcggt gtggctcgcg ctcgcggccg ccccggccgc cgtcctgccg gcccggtaga
      721 acaccacccg gcggccgctc gccgcgatgc gcctgaccgc ccgcagcgtc tttatgccat
      781 cgagcggctt gaagccctcg acgtacacgg cgtagagcat gatcgcttcg tcgtcggcca
      841 ggcgctccag gtaatcgccg acggttacgt ccatctggtt gccacaggtt accaggtact
      901 tcggattcac gtcggggtgc ttggacacgc ggctgatcgc gaaggccccg gactgcgata
      961 tgaaggccac cgggtccacc tggccgctcg gcatcggcag cttgtactcg ggaatgaaca
     1021 gcgtgttgta gcggcctggc accgaccgga tgcccaggca gttgccgccg ttcaggatgg
     1081 ggcctctccc cttcgtcccg cgggaggcgg ccagcgcgtc gcgcatcctg cctacgatgg
     1141 cctcggtgcc ggacttctcc tccaggccgc cgggtatcac gatgacggcc tcggccttgt
     1201 cgcgctcggc cagctcggcg atgcagccgg gcgtctgcgc ggcggggatg ctcaacacga
     1261 acagatcgac gcgctcgggc agcgacgcga cgtcgggcac gcagcgcacc ccgtccacct
     1321 cggcgcagcc tggcttgacc acgtagagcc gcgatcggtc gaagccgttg tcgatcaggt
     1381 tacgcaggat gatgcggcca ttgttcatgc ccttctccga cacgcctatc accgcggcgc
     1441 tcgccgggcg gagcaggcgg tcgatcttgt ccaccggcct cgaagcctgc gcgcggttga
     1501 ccaccgaccc gtcgggcgcc agggacagct catccccggc tatgcggccg agcttggcca
     1561 gcgcatccag cgcgacgacg gagccggaac cgtcggcgcc tcgtaccacc acgaagggat
     1621 tcacctcgag ctcggtgatg ccggccctgg cgaacgccgg cgccgcctcc acaaaggccc
     1681 ataccaggtc gacgaggcgc tcctcgtcta tggtggcctt cgtgccgcgc aggccgccgc
     1741 acagcagctc gcgcaccgcg ttgccgcgta gggcggcccg caccgagtcg cggctggccc
     1801 cgtcggggct caggaacagc gtcgccgcgc ccggcttgaa gctcttggcc aggaactccg
     1861 tatagatgcc gccgggcccg aacgacacga gcggcccgaa gtcccgcgcg aagcggtaac
     1921 ctacgatgat ctcgtgcccg agcctgggct cgaagcgcac gtactcgttg atcgtatagc
     1981 cgtcgacgcg gtaggagccg aagcggccct ccatgtcggc tatcgcggcg cggatcgcct
     2041 caggctcgtt gtccaccatg gcgacgccgc cggcctcggt cttatggagt atctcggggc
     2101 tcaccacctt gaccacgacc cggtcaccgg gcaccgatgc aagcctcgcg acctcggacg
     2161 atccccggac ggccaggctg accggcacgc gtaggcccat cagcccgagt atctccacgc
     2221 cctccggctc cgtcagcgcg tcccgcccct cggccgccgc cgcggcgaca atggcgccta
     2281 gcttcttctc gtcaatcatc gcgacactcc tgcgaaatag cttttacacg atctatcaca
     2341 ctaaggcacc aaggcacgaa gtaccaaaaa ttgtctcgcg cggagacgcg gagacgctaa
     2401 gtacgctata tacgcaaaaa catgaacaag atgtcaggat agaagataga tgaacaagat
     2461 aaaacctagg ccttggcttt tcttatcttg tccatcctgt ccacatcttg gtcatcctgt
     2521 tccacctttt cctaattcct ccgtgccttc gtgtaagaat tacttgagtt cctcacacaa
     2581 aggcactaag ccacaaagga cacgaagaaa acagaacagg atgtcaggat gaaagatgga
     2641 taaacaagat aaaggccaaa gccttagctt ttcttatcct gtccatcctg tccaaatctt
     2701 gtccatcttg ttccgccttt tctctatccc cctccgcgcc tttgcgcctt tgcgcctttg
     2761 cgcctttgcg cctttgcgcc tttgcgcctt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn ncgcctttgc gcctttgcgc ctttgcgcct ttgcgccttt gcgcctttgc
     2941 gcctttgcgc ctttacgcga aatcttctta aaaatgtctc gcgcagagac gcggagacgc
     3001 taagtacgca aaaacataaa caagatgtca ggatgaaaga aagatgaaca agatgaagac
     3061 ctaagcttta gccttagctt ttcttatctt gttcatcctg tccacatctt gtccattttg
     3121 ttccgccttt tccctattct tttgtgcctt cgtgcctccg tgtgagggtt tttacgattc
     3181 ctcacactaa ggcaccaagg caccaagaca cgaagtttta aaatgtctcg cgcagagacg
     3241 cggagacgct aagtacgcaa aaacataaac aagatgtcag gatggaagaa ggatgaacaa
     3301 gatgaaagac taatccaaag ccctcttcat cctgtccatc ctgtccaact cgtgatcatc
     3361 ctaatcttcg cccattaatg cctctcagaa gtacagctcg tactcttggg gtacgggggc
     3421 gttgtcgtcg tccatgacgt tcccgggcta atcgcccggg cgatgcttag gcgccgcatc
     3481 cgtgcggcgt caacgcgccc tccattctag aagtacagct cgtactcttg gggtacgggc
     3541 gcgttgtaca cgtactcggc ttcctgccgc ttcatcgcga tccagctgtc gatgagctcc
     3601 atcgggaagg ccgcctgcag gtacgcgcag tccttcttga ggccggacag cacatggaac
     3661 agcccggtcg ggaaggtgtt cttcggcgtt tccttagtca cggcgtagcc gagcttgacc
     3721 gggtccagcc cgcgcgccac gccgtcgagg ccggcgagca gcatggccgc caggaagtag
     3781 taggtgttcg cggtcgcgtc gccggtgcgg aactcgatgc gcgcctcgcc cttcttgagg
     3841 tagccgggta tgcgcacggc ggccgcgcgg ctgccctgcg cgaaggtggc ggaaaccggc
     3901 gcctcgtagc cgggtaccag gcggcggtag ctgttcgtgc tcgggttgga ccaggccagc
     3961 agcgagcccg acagcgcgtg gctcagcacg ccggccgtgt aggccaggcc ggtccccgac
     4021 agcccgtaca ggccgtcgcc ggggaacacg ctcttgccgt ccttctcgac gtactggtga
     4081 acgtgcatgc cggagccggc gaccttgtac atcggcttgg gcatgaaggt cacgaagagg
     4141 cccagctcct cggccaccga cctgactatc cacttggcca gcaccaccgc gtcgccggcg
     4201 ctcgccagcg atatgaagtt cagctcgatc tccagctggc tcgcggccac ctcgtggtgg
     4261 tggtacttca ccggtatgcc ggccgcctcc atcgcgcgca ccgcctcgtt gcgcagctgt
     4321 ccgtagcggt cgtcgggcgc caggcggtga tagcccttgg agatgccgaa gcgcggcgcg
     4381 tcggcgtagt cgtcgcctat gccctcggcg ctctcgacgt agtagtacga gtggccggcc
     4441 gtcgtcgagt agcgcgcgtc gtcgaacgcg tagaactcga gctccacgag catcttggcg
     4501 tcgtcgccga cgccgctcga gcggagcgcg tccatcgcct tgcgggcgac cgtgcggggg
     4561 tactggtcga agggctcccc gtcggtggtc cagacgtcgc agaacgcgtg caggatcctg
     4621 aagccgtcct tctcctcgac gaagccggtg gacaggtccg gtacggcgac catgtcgctc
     4681 ttgtgtacct gggcgtagcc gaagttgctc gcgtcgaagc cgatgccgcc cttgagcacc
     4741 ttatcgctca cgtaggcccg aggtagcgat accgaccgta ccgcgccgtt gatatcgatg
     4801 accaggagcg acaggtagtc gatgcccgat aggtcgccgg aatacttcga gagcttcatg
     4861 ctcgtctcct tttgcgggaa agggcgatcc ggccgacgtg gcgcgcgaat cgtcgttcga
     4921 gcccggaatg gggcctttcg tagaatagta gcggctcgaa ggcgcttgcg caagtatttt
     4981 gcgctacacc tggttcatta tacgacactg tgtttcgatt attgaaaata ttgacgatat
     5041 agcggccaga aagccggcct cgtcggccgg ccccgccggg gcgggccgat ccgttacgct
     5101 accagccggc cttccggccg gccgctaccc gtgccgtcga ccggccatcg ccgctagctc
     5161 gtcccgccgc gggacacgt
//
LOCUS       DAOPHV010000127         5176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_21348_length_5176_cov_3.653583, whole genome shotgun sequence.
ACCESSION   DAOPHV010000127 DAOPHV010000000
VERSION     DAOPHV010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21348_length_5176_cov_3.653583"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLQ29_04695"
     CDS             complement(<1..211)
                     /locus_tag="PLQ29_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPG85977.1"
                     /translation="MSEQRKRTFEHVIRERWCKGCNVCVSFCPKTVLELRNGKVFAAR
                     PADCIGCRMCELRCPDYAIEIRETGL"
     gene            642..1370
                     /locus_tag="PLQ29_04700"
     CDS             642..1370
                     /locus_tag="PLQ29_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPG85978.1"
                     /translation="MPTSDTPVRSIERALDIMDCFQPDRLELSLTEIATGIELAMSTT
                     SRIVATLEKRHYLSRNPDTQRYSLGSRISQIGALGLSNMDLRRVALPFMKELNALYNE
                     GVSLYIERDNERICIERVESTLPLRRVINVGDRHPLTRGAAGRVLLAYMPADRRQALL
                     SEDPFTTEESLAELRHGGYTVSLGEREEGVTSIAAPIQDAQCAVIAALSMSGPSVRFE
                     GSGFSDKIAKVKRCAELISNALGR"
     gene            1469..3151
                     /locus_tag="PLQ29_04705"
     CDS             1469..3151
                     /locus_tag="PLQ29_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85979.1"
                     /translation="MAKRLLIRDLTLRDGQQSQFATRMNQDQVDRVLPFYREARFYAM
                     EVWGGAVPDSVMRFLNESPWTRLETIRDGVAGASKLTALSRGRNLFGYNPYPDSVIEG
                     FCRNSVRSGIDIMRVFDALNDVDNMTSTIRFVKENGGMADCAVCYTVDPKFTFGDRVG
                     AFFKGKKIPGTLFGVPYFVRKAKELAAAGADMITIKDMAGLVDPATSAELIKALKAEV
                     GVPIDLHTHCTPGYGTASVLMAMVSGVDIVDTVVMNFSGGPAAPAFEIVQLLADRLGI
                     DTGVDLAAVAAINRELGGIRAELSKFDQFKRLPPDIDLTAYEPSPELRSLFDGAAEAA
                     TAGRIDETLRLCQAIEAAFNYPEPDEIVKRAQIPGGMYTNMMSQLKEAKLEQHLEEVL
                     QIVPQVRLDAGVPPLVTPTSQIVGVQAVNCIVDKAQGKPFYSNVSKNFVELVKGSYGR
                     TPFPVDPAFREKVAGVRDEVPYDTSKYKKQDNPELADCGGLRLALNEKEELLLELFPS
                     VADKFLKGRRQAEWQAMEAARLAALPPEPDPDEGARYPSKFWEEELQLAIEG"
     gene            complement(3324..4403)
                     /locus_tag="PLQ29_04710"
     CDS             complement(3324..4403)
                     /locus_tag="PLQ29_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPG85980.1"
                     /translation="MRLTLTENQNAIAPVFLQSRFWADFKVEAGWSYVRYDARLDDGP
                     YAGSIFTLTVMERGLGVGFRFAYVPHGPSVALEPAGRTGLLVALAKALRGLVSGSCLF
                     IRFDPVWYEAEDASGEASSDRLATPPRPVFSAPLRKASDVQPPDSVVLDIARPDDDLL
                     AGMKPKWRYNIRLAEKKGVTVASEGAGSLGEFYALYEATAARDRIAIHPASYYERLFG
                     LEVAGSAEPKPDIRLWMARHEGLALAGIVTVFYGDEATYLYGASGDEHRNLMPAYALQ
                     WAAVKAARDAGCRSYDFYGIPPTDDPKHAMSGLYRFKTGFGGEIRHYAGAWDYVLRPA
                     AYAAFRAAERARLFWHKALRKRLGR"
     gene            complement(4517..>5176)
                     /locus_tag="PLQ29_04715"
     CDS             complement(4517..>5176)
                     /locus_tag="PLQ29_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPG85981.1"
                     /translation="AELRERYSGSQKERAARDADSGKVAAQAKPEKPADKPADKPAEP
                     KPIPYAELPLEERFARLVDLRVAKIVKVERHPKADKLYVETLDDGSGAERVIVSGLVP
                     FYAEEDLLGKHIVLVNNLKPAKLRGVESRGMLLAASRSGDEGKEVVEALEAPWAAPGT
                     RVVLEGQDASVPADAEIDVDAFFSVPIVAKGNTPMVGVKALTADGKNLATTKIPDGEV
                     G"
BASE COUNT          993 a         1690 c         1562 g          931 t
ORIGIN      
        1 cgagtcccgt ctccctgatc tcgatggcgt agtcgggaca cctgagttcg cacatccggc
       61 agccgatgca atcggccggc ctggcggcga aaaccttgcc attgcgtagc tccagtacgg
      121 ttttcgggca gaaggatacg catacattgc atcccttgca ccaacgctcg cgaatgacat
      181 gctcgaaggt acgtttcctt tgttcgctca tatcctgctc tctatggatg atgcgccgtt
      241 acggcgatac gtgattactg cttatacact gcccggctcg tccgaacgga ggcgcgatcg
      301 tcgatacgag gccgtcgttc aacgatagtc ccaagcgatc ggcttgtcaa aaacctcccg
      361 ggcggcgtcc aacgacctcg tttcggcgtc gtctctcgat cgactccgag tttgttaagc
      421 gcggattcca cctggtggaa cccatcaata ccttttccat ttattaaagc atagacctcg
      481 accagagcat tgtcaagtga agctagcacc tattgaaacc actatttcat ctattgacaa
      541 gtgtcactac agacgtgata cttccattat gcgtgactat gttccaccaa gtggaatgat
      601 agccaggcgc cacataaacc aggctatata gaggaacaca tatgccgacg agcgatacac
      661 ccgtcaggtc catcgagcgc gcgctggaca tcatggactg tttccagccg gacaggctgg
      721 agttatcgct gacggagatc gccaccggca tcgaactggc catgagtacg acctcgcgca
      781 tcgtggccac tctcgagaag cgccattacc tgtccaggaa ccccgacacg cagcgctact
      841 cgctcggctc gaggatatcc cagatcggag cgctcggttt atccaacatg gacctgcgca
      901 gggtggccct gccgttcatg aaggagctga acgcgctcta caacgagggt gtcagcctat
      961 atatagaacg cgacaacgag cggatctgca tcgagcgggt cgaaagtacg ctaccgctca
     1021 ggcgagtgat caacgttggc gaccgccatc ccctgaccag gggagccgcc ggcagggtgc
     1081 tcctggccta catgccggcc gatcgtcgtc aggccctact gtccgaagat ccgttcacta
     1141 ccgaggagag cctcgcggag ctgcgccatg gcggctacac ggtcagcctc ggcgagcggg
     1201 aggaaggtgt cacctctatc gcggcgccca tccaggacgc ccagtgcgcc gtgatcgccg
     1261 ccctgtcgat gtcgggcccg tcggtccgct tcgagggctc gggcttctcc gacaagatag
     1321 ccaaggtcaa gcgctgcgcc gagctcatat cgaacgcgct cgggcgatag cggtccgatc
     1381 ggctggatca tcgtccgggc atcctgtata ctggaaggac ggtcgcgtcc tttcgactcg
     1441 cgaccatcat gatactacgg agcatacgat ggctaagcgt ctcttgattc gcgacctgac
     1501 cctgcgcgac ggccagcaat cccagttcgc cacccgaatg aaccaggacc aggtcgaccg
     1561 cgtcctgccg ttctatcgcg aggcccgatt ctacgccatg gaagtctggg gcggcgcagt
     1621 ccccgattcg gtgatgcgct tcctgaacga gagcccctgg accaggctcg agaccatccg
     1681 ggacggcgtg gccggcgcct ccaagctgac cgccctatcc cgcgggcgca acctgttcgg
     1741 ctacaacccc taccccgact ccgtcatcga gggcttctgc aggaactccg tgaggtcggg
     1801 catcgatatc atgcgcgtgt tcgacgcgct gaacgacgtc gacaacatga cgagtaccat
     1861 acgcttcgtc aaggagaacg gcggcatggc cgactgcgcg gtctgctata ccgtcgaccc
     1921 gaagttcacc ttcggggacc gcgtcggcgc cttcttcaag ggaaagaaga tacccggcac
     1981 gctattcggc gtgccctatt tcgtgcgcaa ggccaaggag ctagcggccg ccggcgccga
     2041 catgatcacc atcaaggaca tggccggcct cgtcgacccc gcgaccagcg ccgagctgat
     2101 caaggccctc aaggccgagg tcggcgtgcc catagatctg cacacccact gtacgcccgg
     2161 ttacgggacg gcgagcgtcc tgatggccat ggtctcgggc gtcgacatcg tcgacacggt
     2221 ggtcatgaat ttctccggag gccccgcggc gccggccttc gagatcgtcc agctcctggc
     2281 cgaccggctc ggcatcgata ccggcgtcga cctggcggcc gtagcggcta tcaaccgcga
     2341 gctaggcggc atacgcgccg agctgtccaa attcgaccag ttcaagcgcc tacccccgga
     2401 tattgacctg accgcctacg aaccgagccc ggagttgcgt tccctgttcg acggggcggc
     2461 cgaggccgct acggccggta ggatagacga gacgctcagg ctctgccagg ccatcgaggc
     2521 cgcgttcaac taccccgagc ccgacgagat cgtcaaacgc gcccagatcc ccggcggcat
     2581 gtacacgaac atgatgtcgc agctcaagga agccaagctc gagcagcacc tcgaggaagt
     2641 gctccagatc gtgccgcagg tcaggctcga cgccggcgta ccgccgctcg tcaccccgac
     2701 cagccagatc gtcggcgtgc aggcggtcaa ctgcatcgtc gacaaggccc agggcaagcc
     2761 cttctattcg aacgtctcca agaacttcgt cgagctggtc aagggctcct acggacgcac
     2821 gcccttcccg gtcgacccgg ccttccgcga gaaggtagcc ggcgtacgag acgaggtgcc
     2881 ctacgacacg agcaagtaca agaagcagga caatcccgag ctggccgact gcggtggcct
     2941 caggctcgcg ctcaacgaga aggaggaact cctcctcgag ctgttcccga gcgtggccga
     3001 caaattcctg aagggtcgga ggcaggccga atggcaggcg atggaggcag cgcgactagc
     3061 cgccttgccg cctgaaccgg atccggacga aggcgcgcgg tatccgtcca aattctggga
     3121 agaggaactg cagctggcca tcgagggctg atagcgatag gcggagaacg aaggaggcgg
     3181 tcgaaaggcc gcctcctcca ttcaatcccc gctagcgatt ccctatggtc gacggtacct
     3241 tcgaactaat ccaggacaca tcgaaacctc gtagtgtgga cgctttcatc cagataccgg
     3301 catagaggcg atcggtctcc gtatcatctc cccagccgct tcctaagcgc tttatgccag
     3361 aacagtctcg ctcgctcggc cgccctgaac gccgcgtagg ccgccggcct gagcacgtag
     3421 tcccaagcgc ccgcatagtg gcggatctcg ccaccgaagc cggtcttgaa gcgatagagg
     3481 ccggacatcg catgcttcgg gtcgtcggtc gggggtatac cgtagaagtc gtagctacgg
     3541 caccccgcgt ccctggcagc cttgacggcg gcccattgga gcgcgtaggc gggcatcagg
     3601 ttgcgatgct cgtcgcccga cgcgccgtac aggtaggtgg cctcgtcgcc gtagaagacg
     3661 gtgacgatac cggccaaggc cagtccttcg tggcgcgcca tccacagcct gatatccggc
     3721 ttcggttcgg ccgagcccgc gacctcgagt ccgaacagac gctcgtaata cgaggcggga
     3781 tggatggcta tccgatcgcg tgccgccgtc gcctcgtaga gcgcgtagaa ctctcccagc
     3841 gagccggctc cctccgaggc taccgtcacg cctttcttct ccgcgagcct gatattgtag
     3901 cgccactttg gcttcatgcc ggcgagcagg tcgtcgtcag gcctcgcgat gtcgaggacg
     3961 acgctgtcag gcggctgcac gtcggaggcc ttgcgcagcg gcgcgctaaa gaccggccta
     4021 ggaggcgtag cgagtctgtc cgaggacgct tcgcctgaag cgtcctcggc ctcgtaccag
     4081 accggatcga agcgtatgaa caggcaggat cccgatacga ggccgcgcaa ggccttggcc
     4141 agggccacga gaagccccgt ccgtccggcc ggctccaagg ccacggacgg tccgtgcggt
     4201 acgtaggcga aacggaaacc gaccccgagg ccgcgttcca tcacggtcag cgtgaagatc
     4261 gagccggcgt atggcccgtc gtcgagccgc gcgtcgtagc ggacgtagct ccaaccagcc
     4321 tccaccttga aatccgccca gaatcgactc tggagaaata cgggagcgat ggcgttttga
     4381 ttctcagtta gggtcaggcg catctgcatg tatccttttt aagacgaagg ggcggcgaaa
     4441 tcagccttga ttcgccgccc cgacatacat ttctgacatc acgaggctta tttcagccgc
     4501 tgagcctatc ggaagctcag cccacctcgc cgtcgggaat cttcgtggta gcgaggttct
     4561 tgccatcagc cgtcagggcc ttgactccga ccatcggcgt attgcccttg gccacgatag
     4621 gcaccgagaa gaacgcgtcc acatcgatct ccgcatcggc cggaacggag gcgtcctgcc
     4681 cctcgagcac gacgcgggtg ccgggcgccg cccagggcgc ctcgagcgct tcgacaactt
     4741 ccttgccttc gtcgccggaa cgcgaggccg ccaggagcat gccccgggat tcgacgccgc
     4801 gtagcttggc cggcttgagg ttgttgacga gcacgatgtg cttgccgagt agatcctcct
     4861 ctgcgtagaa cggcacgagg cccgatacta tcacgcgctc ggcgccggag ccgtcgtcga
     4921 gcgtctcgac atacagcttg tcggccttgg ggtggcgctc gactttgact attttcgcca
     4981 cccgcaggtc gacgagccgg gcgaagcgct cctcgagcgg cagctcggcg tatgggatgg
     5041 gcttcggctc cgcgggcttg tccgcaggct tgtccgcggg cttctcaggc ttagcctgag
     5101 cggcgacctt ccccgagtcg gcgtcgcgag ccgcccgctc cttctgcgag cccgaatagc
     5161 gctcgcgcag ctcggc
//
LOCUS       DAOPHV010000128         5168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_21395_length_5168_cov_3.505965, whole genome shotgun sequence.
ACCESSION   DAOPHV010000128 DAOPHV010000000
VERSION     DAOPHV010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21395_length_5168_cov_3.505965"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PLQ29_04720"
     CDS             complement(<1..640)
                     /locus_tag="PLQ29_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HPG85982.1"
                     /translation="MNHSLLEHHSEEELWAAYRQRKDPLIREAFIKQYAPLVKYVAGK
                     VVANMPSSVEFDDLVGFGVFGLIDAIDKYDPDKNVKFKTYAVTRIRGAIYDELRSIDW
                     VPRSVRQKTREIEEAIVQTEAKLGRPATDQEIAESLGLSDDEFSKTMLKISSTSILSL
                     NDVWYTADESEHMTIGDSVESPGSMSPDSITEREEIRRVIVEAIQELPDKEKK"
     gene            complement(641..1333)
                     /locus_tag="PLQ29_04725"
     CDS             complement(641..1333)
                     /locus_tag="PLQ29_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85983.1"
                     /translation="MDSSLRASAFAGLGAFALSIIVAAFSRVPFGALVLRALLSGLAF
                     AGLCYGAIMVLRRFLPELFGDVAATAYTGQDESVGSIVDIVLPGGEAERDVQAAEAGE
                     SAPAVAAEPRREPAATAVAAGELEREVADLRSDSFVSSDPGEAPSPNGAPRPSVSLDE
                     LDTLPDLDGFSDAFTTTLAGEGEAPAPDASYLAGPSDSMPSAGAPDAGRDPAVLAKAV
                     QTLLRRDQKGQL"
     gene            complement(1419..2285)
                     /locus_tag="PLQ29_04730"
     CDS             complement(1419..2285)
                     /locus_tag="PLQ29_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HPG85984.1"
                     /translation="MEDQAQKLRELMSAKPQPQQAAKKTRVITVASGKGGVGKTNLSV
                     NLAIAYARTGKRVMVMDADLGLANVNIMLNMIPKYNLYHVIRKQKSMRDIILDTEYGI
                     QILAGASGFSKIANLNEEERQNFISELYALTNVDIIIIDTSAGVSNNVLSFVAAADDA
                     IIITTPEPTAITDAYGIIKIIATEVENLNIGLKLVVNRVKTVTEGRKVAERIINISGQ
                     FLNLKVEYLGCIYEDPIVQQSVLRQRPFIVQDPKSKAAISVQHLVNRIEKTEFVDDRG
                     ISRLIKKLFSKE"
     gene            complement(2311..3489)
                     /gene="flhF"
                     /locus_tag="PLQ29_04735"
     CDS             complement(2311..3489)
                     /gene="flhF"
                     /locus_tag="PLQ29_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HPG85985.1"
                     /translation="MQYLVEQGSSHREVLEKVRARYGDAAQVLSHRTVRSGGFLGLFA
                     REGVEITFYLKDDAVREGEKKKADIEEEKRRLLERVNQERAIQEVLSEVRSLKDTLAE
                     SVARPAAQDGEHPTLAALDALLVDNDFSPWYRAELLARARDEFSLGELEDYAAVEEAF
                     LEWIGDTVRIPAPARPVKPRVLVLVGPTGVGKTTTIAKLAALHSLGIGGLPAGAVRMI
                     TIDSYRIGAVEQIEKYAGIMRVPVSVVETAQDLRETIAMYRDNDLILVDTIGKSPRDA
                     IRLAEMQQVLDACGPAAEVYLAVAATTKTQDMLDIFRQFEPFRYRGVVVTKMDETSRV
                     GNVLSVLAETDKAVAWITDGQKVPQDIARANPLRFLMNLEGFRPNRPKLEERYGSTTT
                     "
     gene            complement(3493..5168)
                     /locus_tag="PLQ29_04740"
                     /pseudo
     CDS             complement(3493..5168)
                     /locus_tag="PLQ29_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189099.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
BASE COUNT          834 a         1633 c         1653 g         1048 t
ORIGIN      
        1 ccttcttctc cttatccggt agctcctgga tggcctcgac gatcacgcgt cgtatctcct
       61 cgcgctcggt gatcgagtcg gggctcatgc tgccggggct ctcgacgctg tcgcctatcg
      121 tcatgtgctc ggactcgtcc gcggtatacc agacgtcatt gagcgacagg atcgaggtgc
      181 tcgatatctt gagcatcgtc ttggagaatt catcgtcgga caggccgagc gactcggcga
      241 tctcctgatc cgtagcgggg cggccgagct tggcctcggt ctgtacgatg gcttcttcga
      301 tctccctggt cttctgacgc accgagcgtg ggacccaatc gatactgcgc agctcgtcgt
      361 agatggcgcc cctgatacgg gtgacggcgt aggtcttgaa tttaacgttc ttatcggggt
      421 cgtacttgtc tatggcgtcg ataaggccga acacgccgaa cccgaccaga tcgtcgaact
      481 cgaccgatga cggcatgttc gcgacgacct tgcccgccac atacttgacc agcggcgcgt
      541 actgcttgat gaaggcttcg cggataaggg gatccttgcg ctgccggtag gcggcccaga
      601 gctcctcttc gctgtgatgc tccagcaggg aatgattcat ttataattgc cctttctggt
      661 ctctgcgcag taatgtctgt acggccttgg cgagtaccgc cggatccctt ccggcgtccg
      721 gcgccccggc gctaggcatg ctgtccgacg gccccgccag gtacgaggcg tcgggagccg
      781 gcgcctctcc ttcgccggcc agggtcgtcg tgaaggcgtc gctaaacccg tccaagtcgg
      841 gcaacgtgtc cagttcgtcg agagagaccg acggccgcgg cgccccattc ggcgagggcg
      901 cctcgccggg gtcgctcgag acgaagctat cgctccgcag atcggctacc tcccgctcta
      961 gttccccggc ggcgacagcc gtggcggcgg gctctctccg aggctcggcg gccacggccg
     1021 gggccgattc gcccgcctcg gcggcctgta catcgcgttc ggcctccccg ccgggcagga
     1081 cgatgtcgac gatggagccg acagactcgt cttgccccgt gtaggccgtc gccgcgacgt
     1141 ctccgaacag ctcggggagg aatcgtctca gtaccatgat ggcgccgtag catagccccg
     1201 cgaaggctag gcccgacagc agggcgcgga ggaccaacgc gccgaaaggg acgcgcgaga
     1261 aagcggcgac gatgatcgat aacgcgaacg cgccaagccc cgcgaaggcg gacgcgcgaa
     1321 gactcgagtc caccgtttca gtaccctccc atagccgaca acattaaggc ataatacctt
     1381 gtctcgtcaa gctttggctc tcggtacgct ccgcggcgct attctttcga gaacagcttc
     1441 ttgatgaggc ggctaatgcc gcggtcgtcg acgaattcgg tcttctcgat acggttcacc
     1501 aggtgctgca ccgagattgc ggccttggac ttgggatcct ggacgatgaa gggccgctgc
     1561 ctgagcaccg actgctggac gatggggtct tcatagatgc agccgaggta ttcgaccttc
     1621 aggttgagga actgccccga gatgttgatg atacgttcgg ccaccttgcg gccttcggtc
     1681 acggtcttca cgcggttgac gaccagcttg aggcctatgt tcaggttctc gacctcggtg
     1741 gctatgatct tgatgatgcc gtaggcgtcg gtaatggcgg tcggctccgg ggtggtgata
     1801 atgatagcgt cgtcggccgc ggcgacgaag gacagcacgt tgttcgagac gccggcgctc
     1861 gtatcgatga tgatgatgtc gacattggtc agagcgtaca gctcggatat aaaattctgc
     1921 cgttcttcct cgttcaggtt ggcgatcttg gagaagcccg acgccccagc caggatctgg
     1981 atcccgtact ccgtatccag gatgatgtcg cgcatagatt tctgcttacg tatcacatgg
     2041 tacaggttgt acttgggaat catgttgagc atgatgttga cgttggccag gccgaggtcc
     2101 gcgtccatta ccatcacgcg cttgccggtc cgggcgtagg ctatggctag gttgacggac
     2161 aggttcgtct taccgacgcc gcccttgccg ctggcgacgg tgatgacgcg ggtcttcttg
     2221 gccgcctgct ggggctgcgg cttagcgctc atcagctccc gcagcttctg cgcctggtct
     2281 tccatacgag cactccatcg atacggcggg ttacgtagtc gttgatccgt atctctcttc
     2341 tagtttcggt cgattcggcc taaaaccttc gagattcatg aggaatctga gaggatttgc
     2401 ccgcgcgatg tcctggggca ccttctggcc gtcggtaatc caggccacgg ccttgtcggt
     2461 ctcagcgagc accgaaagca cgtttccgac gcggctcgtc tcgtccatct tcgtaacgac
     2521 cacaccccga taccggaacg gctcgaactg cctgaagata tcgagcatgt cctgagtctt
     2581 ggtcgtagcg gccaccgcga ggtagacctc ggcggccggc ccgcaggcat cgagcacctg
     2641 ctgcatctcg gccagccgta tcgcgtcccg ggggctcttg cctatcgtat cgaccagaat
     2701 aaggtcattg tcacggtaca tcgctatggt ctcgcgcagg tcctgggccg tctcgaccac
     2761 ggagaccggc acgcgcatga tcccggcgta cttctcgatc tgctccaccg ccccaatgcg
     2821 gtagctgtct atggtgatca tgcgcacggc gccggccggc aggccgccaa tgccgaggct
     2881 gtgcagggcc gccagcttgg cgatggtcgt cgtcttgccg acgccggtcg ggcctacgag
     2941 cacgagcacc cgcggcttga ccggcctcgc gggcgcgggg atacgtacgg tatcgcctat
     3001 ccattcgagg aaggcctctt cgaccgccgc gtagtcctcg agttcgccga ggctgaattc
     3061 gtcccgggcc ctggccagga gctcggcgcg gtaccagggc gagaaatcat tgtcgacgag
     3121 cagggcgtcg agggcggcga gcgtcggatg ctcgccgtcc tgggccgcgg gcctcgcgac
     3181 gctctcggcg agcgtatcct tgagcgagcg cacctcggag agtacctcct ggatggcccg
     3241 ctcctgattg acgcgctcga gcagcctgcg cttctcctcc tcgatgtcgg ccttcttctt
     3301 ttcgccctcg cgcaccgcgt cgtccttcag gtagaaggtt atctcgacgc cctcgcgggc
     3361 gaacaggccg aggaagccgc cggatcgtac ggtacgatgg ctcaggacct gggccgcgtc
     3421 gccgtagcgg gcgcggacct tctccaggac ctcccgatga ctggagccct gctctaccag
     3481 gtattgcata atttatcctt caacgcgtat ctctccgatg gcctctacct ggatgtcggc
     3541 cacgatctcc ggcaccgata gcacgacgag gtcgggtatc tcccgctccg tgctcgactt
     3601 gacgagggcc ctggccgcct cggagcagag gatcacgggg aaatgcccct gtttctgcac
     3661 cgcggtgacg gcgagcgtca aggccttgat ccattggcgc tgggtcgccg gctccagggc
     3721 cgacatcgcg ccggcgggcg tgtccacccg cgcgtcgatg atacgctgct cgagtgaggg
     3781 ctctatggtc agcacgcgca tcgtcttgtc ctggtcggcg tactggaggc aaatctggcg
     3841 gcccagggac tgcctggact tttcgacgag gaagcccggg tccttcgtga tacctgcgta
     3901 gtcggcgagg gtctcgagta tggcgacgag gttgcgtatc gatacctgct cgcgcaggag
     3961 gccctgaagc accttctgga tctcgccgag cgacaggtgc ttctgcgcgt cctcgacgac
     4021 cgccggatag tcttttttga gggcgtcgag catgccctgg atctcctgcc gcccgaggat
     4081 atcggcggcg tggcgcttga tgatctcggt caggtgagtc gcgacgatgc tcggcgcgtc
     4141 gacgaccgtg tagcccgccc gctcggcccg atcgcgcgcg tcttcagata tccagaccgc
     4201 gggcagcccg aaggcggggt ccttggtgcg ctcgccctga acctcctcgg acacgccgcc
     4261 ggggttgatc gccaggtagt agccgaggcg tatgacgccg cgccccatct cgacgccgcg
     4321 tatcttgaag cagtactccg acggctcgag ccgcatgttg tcgatgatgc ggatacgagg
     4381 gacgacgagg cccatgtcta tggcgctctc cttgcgtatc cttgtgacgc gctcgagcag
     4441 ctccgcgccc ttgtccttgt cgacgagcgg tatgaggccg tagccgagct cgaggctgat
     4501 cgggtcgagc ggcacgaccg gcgacagctc ctgggacgac tcgggcttct tggcgccgcc
     4561 ctcggcagcc ctcagcttgg ccttgtcgga ctcgaggaca gccttcttcg agaggttcca
     4621 ggccaggtac gccatagcgg ccgccatcgg caggagtacg taccacggga agccgggcag
     4681 gagcgcgagg gcgctcagca ccgccgcgcc cacccagtag atcctggcgt tctgcgtgaa
     4741 ctgcgcggtg acctcggagc cgaaggtgcc ttggctattg gcccgcgtga cgatgatgcc
     4801 ggtggagacg gagatgagca gcgcaggcag ctgggagacg agcccgtcgc ctatcgtcag
     4861 cgaggtatag gtgttcagtg ccgaggcgaa cggctcgcca tggagcacca tgccgacgat
     4921 gaagccgcct atcaggttga tcacgctgat gaagatgccg acggtcacgt tgcccgagac
     4981 gaacttgctg gcaccgtcca tctggccgta gaagtcgacc tcgcgctgga cgtcgtcctt
     5041 gcggcgcctg gcctcttcct cgctgatggc gcccgagttg tattctgcct cgatggccat
     5101 ctgcttgacg gcatgccgtc cagggtgaag cgggccgcca cctcggcgat acgcgtcgcg
     5161 cccttggt
//
LOCUS       DAOPHV010000129         5150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_21521_length_5150_cov_3.766438, whole genome shotgun sequence.
ACCESSION   DAOPHV010000129 DAOPHV010000000
VERSION     DAOPHV010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21521_length_5150_cov_3.766438"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLQ29_04745"
     CDS             <1..292
                     /locus_tag="PLQ29_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85986.1"
                     /translation="EDPEGEPLAGVFELERLFGRFASVPGYCLRFVGEGDDASFNAVQ
                     TPLLLAELESIDATAFGAAERAELVRLVALCRKYAGKERTHIRFYGEASSSE"
     gene            289..1197
                     /gene="nudC"
                     /locus_tag="PLQ29_04750"
     CDS             289..1197
                     /gene="nudC"
                     /locus_tag="PLQ29_04750"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HPG85987.1"
                     /translation="MSEPNDTMDTVASAIAEASPSETTRRFAFRGTEALVRASAFDGY
                     PAPDRGTMAGASPWEAPGDELLAVALDSSAAPLPEPGDSLTVPGGALAASYPAETDAP
                     DGWEWKPFRSLINALPEPLWLPAARAMAYANWRASSRFCGRCGSPNGDKPDEIARLCP
                     VCGALSFPRLSPAVLAAVHKDDRLLLARNALNRTGAWSILAGFVEPGESFEGCVGREV
                     MEEVGIVVRTTGYRGSQPWPFPDQLMVGFTAEWVSGELTPDGVEIAEAGWFGPDDHPP
                     LPMRGSLSRRLIEAAFEDIRTAGTRR"
     gene            complement(1172..1894)
                     /locus_tag="PLQ29_04755"
     CDS             complement(1172..1894)
                     /locus_tag="PLQ29_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HPG85988.1"
                     /translation="MTRTIEQAVDGLVVKALRTWDMIRSGDRVLIGASGGKDSAVMAR
                     DLALKKRHGRLDAELVGLHIRNDFSPNALSRRLEDEFAEWGIPLVTVDVAVEGRLKPG
                     RKMNCYWCSTQRRTELIRYAMAEGFSAVALGHHLDDALETLFMNMMRKGELSTMPPVV
                     SYRKYPLRVIRPLYLVEERQVVELAEALGLASATCSCGYNDRSERDRVRDRVEALTGG
                     SSRAKRNLLESLRHVNAEYLPS"
     gene            2042..3073
                     /locus_tag="PLQ29_04760"
     CDS             2042..3073
                     /locus_tag="PLQ29_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPG85989.1"
                     /translation="MGNETTASSGAFGETELTPRHSNGLAILALNILLMIGALVLFIV
                     CVIALDGGGATPLAIIGVIGSLLWLCVVGPILFGGIKVLKPNEAMVFTLFGKYYGVLR
                     EPGIFFVNPFASAVHPASTVELSTASTVDKAVATGRPAGTTVKTVSRTLSLKAMTLNN
                     DKQKINDSLGNPIIIGIVVVWKVVNPTKAVFNVDNYAEYLSIQCDSALRNIVRLYPYD
                     SSDSEGETSLRGSSQEIAEKLRAEIQAKAEAAGIEILEARITHLSYAPEIAAAMLQRQ
                     QASAVIAARQMIVDGAVGMVELALDKLKANNIVDLDEERKAAMVSNLLVVLCSNKDVS
                     PIVNSGSIY"
     gene            3086..3280
                     /locus_tag="PLQ29_04765"
     CDS             3086..3280
                     /locus_tag="PLQ29_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HPG85990.1"
                     /translation="MSGDKEKDKKQILLRLSPSLWRELAAWAEDDFRSINGQIEFLLT
                     ESVRRRRKGDATDWDDGRDE"
     gene            3302..4042
                     /locus_tag="PLQ29_04770"
     CDS             3302..4042
                     /locus_tag="PLQ29_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG85991.1"
                     /translation="MIELKGISKTYAKSAVKAVDDLSLTIEDGEIFGFLGPNGAGKTT
                     TIKVLTGVLRPDSGRASVDGVDIAVDPLEAKRRIGYVTDNPELFARLKAAEYLNFVGD
                     VYGVDSETRRARVAEYAGLFGIRDALGGSIGSFSHGMKQKLIVTASLLPDPPNWVLDE
                     PMVGLDPKAAFALKEVMRARASAGKTVFFSTHVMEVAERLCDRLAIINKGRIVFVGTL
                     AELKSSHGADGSLESLFLELVDDQGEGA"
     gene            4039..>5150
                     /locus_tag="PLQ29_04775"
     CDS             4039..>5150
                     /locus_tag="PLQ29_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85992.1"
                     /translation="MKSFLSLARLYLQSFYNLPAKKAPGAGRDAKALLKAVGIAALVV
                     IVVGEFGFIFVMLNLGMYEGLAAAGMQGMLILNAVVIATMMTLVVGFMTALSTYYVND
                     MELQLLSMPIRPRAMFGAKFLAVYVSEALFSLFFMGIAMVIFGVKEGPGPLFYLWGLV
                     AALLLPLPPLAAAYIIQIPLMSFARFLKNKRVIVMLGGVLGLVFALGFNLYYQRMMVK
                     LGDPAALAAMVGGTDSLVSRIGYSYPPAAFAWQAMSEPASGRAVLSMLGLVGTCVALP
                     ALALLFLSGAYVRSLVGFNEAHIKKLTRAGADAFIVRRIRAGSPFANLVAREFNMMNR
                     EPMYLLNGPFIIVLMPVIIGIMLVAQKDAFMSDPDMA"
BASE COUNT          871 a         1681 c         1659 g          939 t
ORIGIN      
        1 cgaggatccg gagggagagc cgctcgccgg agtcttcgag cttgagcgtc tgttcgggcg
       61 tttcgcgtcg gtacccggct actgcctccg cttcgtgggt gagggcgacg acgcatcgtt
      121 caacgcggtc cagaccccgt tgctgttggc ggagctcgag tccatcgacg ccaccgcgtt
      181 cggcgcggcg gagcgggccg agctggtgcg tctcgtcgcg ctgtgccgta aatacgcggg
      241 caaggagcgc acgcatatcc gcttctacgg cgaggcgtcg tcatcggaat gagcgagccg
      301 aacgatacga tggatacggt agcttcggcc atcgccgaag ccagcccgag cgagaccacg
      361 cgacgcttcg cgttccgagg caccgaggcg ttggtccggg cctccgcgtt cgacggctac
      421 ccggcgcccg atagagggac gatggccggc gcgtcgccgt gggaggcgcc aggggacgag
      481 cttctcgccg tggcgctgga cagttccgcc gcgccgctgc ccgagcccgg ggacagcctg
      541 accgtacccg gcggcgcctt ggccgcgtcg taccccgccg agaccgacgc gccggacggc
      601 tgggagtgga aaccgttccg ctccctgatc aacgccctgc ccgagcccct ttggctaccg
      661 gccgcccggg ccatggccta cgccaattgg cgggcctcga gccgcttctg cggccgctgc
      721 ggctctccga atggcgacaa gcccgacgag atagccaggc tctgcccggt ctgcggcgcc
      781 ctgtcgttcc cgcgcctatc gcccgcggtg ctcgccgctg tacataagga tgaccgttta
      841 ctactagcgc gcaacgcgct gaaccgaact ggcgcgtgga gcatcctggc cggcttcgtc
      901 gagcccggcg agagcttcga gggctgcgtc ggtcgcgagg tgatggaaga ggtcggcatc
      961 gtggtccgta cgaccgggta ccgcggctcc cagccctggc ccttccccga ccagctgatg
     1021 gtcggcttca cggcggagtg ggtctcgggc gaactcactc cggacggcgt cgagatagcc
     1081 gaggcgggct ggttcggtcc cgacgaccat ccgccgctac cgatgcgcgg cagcctgtcg
     1141 cggcggctca tcgaggcggc cttcgaggat atcaggacgg caggtactcg gcgttgacgt
     1201 gcctgagcga ctcgagcagg ttgcgcttgg cacgcgagga gccgccggtc aacgcctcga
     1261 cccgatcgcg tacgcggtcg cgctccgagc ggtcgttgta gccgcagcta caggtcgccg
     1321 acgcgagtcc cagcgcctcc gccagctcga cgacctgccg ttcctccacc aagtacagtg
     1381 ggcgtatcac ccggagcgga tacttgcgat agctcaccac cggcggcatc gtcgacagct
     1441 cgcccttgcg catcatgttc atgaacagcg tctcgagcgc gtcgtccaag tgatggccga
     1501 gcgccaccgc ggagaagccc tcggccatag cgtagcgtat caactcggtg cgccgctggg
     1561 tggagcacca gtagcagttc atcttccggc ccggcttgag cctgccctcg accgccacgt
     1621 cgaccgtgac cagcggtatt ccccattcgg cgaattcgtc ctcgagtcgc ctcgacaggg
     1681 cgttcggcga gaagtcgttg cgtatatgga ggccgaccag ctcggcgtcc aggcgtccat
     1741 ggcgcttctt aagcgccagg tcccgcgcca tgacggccga gtccttgccg cccgaggcgc
     1801 cgatcagtac gcgatcgccc gaccgtatca tgtcccaggt cctaagcgcc ttgacgacga
     1861 ggccgtcgac cgcctgctct atggttcgtg tcacggttcg ttctactatc gatgcgcggc
     1921 ccgccgcaag cccagtccgc atcgaattcg ccataggcct cttgaattcc ggcgcgcgta
     1981 acgctattat ctagatgata tcatattgat atcttttagg tatcgttata aggagacctc
     2041 catgggtaac gaaacgactg catcttcggg agccttcggc gagacggagc tgacgcctcg
     2101 acattcgaac gggctggcca ttctggctct gaatatcctg ttgatgatag gggcgctcgt
     2161 cctgttcatc gtatgcgtga tcgccctgga cggcggaggc gcgactccgc tcgctattat
     2221 cggagtgata ggttccctgc tctggctgtg cgtcgtcgga cccatcctgt tcggcggcat
     2281 caaggtgctc aagccgaacg aggcgatggt cttcacgctg ttcggcaagt actatggcgt
     2341 gctccgggaa cccggcatct tcttcgtgaa cccattcgcg tcggcggtcc atccggcctc
     2401 gaccgtcgag ctgtccaccg cctcgaccgt cgacaaggcg gtcgcgacgg ggcgtccggc
     2461 cgggacgacg gtcaagaccg tctccaggac tctgtcactc aaggcgatga cgctcaataa
     2521 cgacaagcag aagatcaacg actcgctcgg caacccgatc atcatcggca tcgtggtcgt
     2581 ctggaaggtg gtcaacccga ccaaggcggt gttcaatgtc gacaactacg ccgagtacct
     2641 atcgatccaa tgcgactccg cgctgcggaa catcgtgcgc ctgtacccct acgattccag
     2701 cgactccgag ggcgagacct cgctccgggg cagcagccag gaaatcgccg agaagctcag
     2761 ggccgagatt caggccaagg ccgaggcggc cggcatcgag atcctcgagg cgcgcatcac
     2821 gcacctgtcg tacgcgccgg agatcgccgc cgcgatgctg cagcgccagc aggcttcggc
     2881 ggtcatcgcc gccagacaga tgatcgtcga cggcgcggtc ggtatggtgg aactggcact
     2941 ggacaagctc aaggcgaaca acatcgtcga cctggacgag gagcggaagg ccgcgatggt
     3001 cagcaacctg ctcgtcgtgc tctgctcgaa taaggacgtg tcgccgatcg tgaattctgg
     3061 ctcgatctat taggcgtagg gactcatgag cggcgacaag gaaaaggata aaaagcagat
     3121 actcttgcgg ctgtcgccgt cgttgtggcg ggagctcgcc gcctgggccg aggacgactt
     3181 caggtccatc aatgggcaga tcgagttcct gctgaccgag agcgtgcgcc gccgccgtaa
     3241 gggcgatgcg acggattggg acgatggccg cgacgagtag cggccggtgg ggagagacat
     3301 catgatagaa ctgaagggca tttctaagac ctacgccaaa agcgccgtca aggcggtgga
     3361 cgacctgtcg ctgaccatcg aggacggcga gatcttcggc ttcctgggcc cgaacggagc
     3421 gggcaagacg acgacgatca aggtgctgac cggcgtactg cgccccgact cgggccgcgc
     3481 ctcggtcgac ggagtcgaca tagcggtcga cccgctcgag gcgaaacgcc gcatcggcta
     3541 cgtgaccgac aatccggagc tgttcgccag gctcaaggcc gccgagtacc tcaacttcgt
     3601 cggcgacgtg tacggcgtcg attccgagac gcgccgggcc agggtggcgg agtacgccgg
     3661 cctgttcggc attcgcgacg cgctcggagg cagcatcggc agcttctccc acggcatgaa
     3721 gcagaagctc atcgtgacgg cgagcctcct gcccgacccg ccgaactggg tgctcgacga
     3781 gccgatggtc ggccttgacc cgaaggcggc cttcgccctc aaggaggtca tgcgggctag
     3841 ggcgagcgct ggcaagacgg tcttcttctc gacgcacgtg atggaggtgg ccgagcgcct
     3901 gtgcgacagg ctcgccatca tcaacaaggg caggatcgtg ttcgtcggta ccctggccga
     3961 gctcaagtcg agccacgggg ctgacgggtc gctcgagagc ctgttcctgg agctggtcga
     4021 cgaccagggg gagggggcat gaaatccttc ctgagccttg ctcggctcta cctccagagc
     4081 ttctataacc tgccggccaa gaaagcgccc ggagccggac gcgatgccaa ggccctgctc
     4141 aaggcggtcg gcatagccgc gctggtcgtc atcgtggtcg gcgaattcgg cttcatcttc
     4201 gtcatgctca acctgggcat gtacgagggc ctcgccgccg cgggcatgca gggcatgctc
     4261 atcctgaacg ccgtggtcat cgcgacgatg atgaccctgg tcgtcggctt catgaccgcg
     4321 ctgtcgacct actacgtgaa cgacatggag cttcagctcc tgtcgatgcc gatacggcct
     4381 cgagccatgt tcggcgcaaa gttcctagcg gtctatgtgt ccgaggcgct gttctccctg
     4441 ttcttcatgg gcatcgcgat ggtcatcttc ggcgtgaagg aaggcccggg gccgctgttc
     4501 tacctctggg gcctcgtagc agcgctactc ttgcccctgc ctccgctcgc ggcggcgtac
     4561 atcattcaga tacctctgat gtcgttcgcg cgtttcctca agaacaagcg cgtcatcgtg
     4621 atgctcggcg gcgtgctcgg cctcgtcttc gcgctcggct tcaatctcta ctaccagcgt
     4681 atgatggtca agctcggcga tccggccgcc ctcgccgcca tggtcggcgg cactgactcc
     4741 ctggtctcca ggataggata ctcctatccc ccggccgcgt tcgcgtggca ggccatgtcg
     4801 gagcccgcta gcggccgggc cgtcctgtcg atgctcggcc tcgtcggcac ttgcgtagcc
     4861 ctaccggccc tggccctgct gttcttgtcc ggggcctacg tgcgtagcct cgtcggcttc
     4921 aacgaggccc atatcaagaa gctgacgcga gccggcgccg acgcctttat agtcaggcgc
     4981 atccgagcgg gcagcccctt cgcgaacctc gtggcgcgcg agttcaacat gatgaaccgc
     5041 gaacccatgt atctgctcaa cggccctttc atcatcgtgc tcatgccggt catcatcggc
     5101 atcatgctcg tggcgcagaa ggatgccttc atgtccgacc cggacatggc
//
LOCUS       DAOPHV010000130         5145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_21554_length_5145_cov_3.821022, whole genome shotgun sequence.
ACCESSION   DAOPHV010000130 DAOPHV010000000
VERSION     DAOPHV010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21554_length_5145_cov_3.821022"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PLQ29_04780"
     CDS             <1..526
                     /locus_tag="PLQ29_04780"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPG85993.1"
                     /translation="RLLADGMTPNPLLTRIGLNTGDMVVGNMGTERKMDYTIMGNAVN
                     LAARLEGVNKQYGSWILMSDATYQEAGEEFLARRLDRVRVVGINTPVQLWELVGLKAD
                     TDEGTLDFLARFEEAHKTFDGRDWKKAARLLSALAAERPDDGPTKSYIKKCEVFTLKP
                     PASDWDGVFSLTEK"
     gene            686..1594
                     /locus_tag="PLQ29_04785"
     CDS             686..1594
                     /locus_tag="PLQ29_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPG85994.1"
                     /translation="MHDYRAVRLIRGAKAVVEGAGVRLYRAVGLGDPEECDPFLLLDD
                     FRSDDPRDYSRGFPWHPHRGIETITYVLKGTVEHEDSIGNKGVIGPGDVQWMTAGSGI
                     FHQEMPRGDGDGSMHGFQLWANLPASDKMKEPRYRGIRGEDIPKVTTPNGVVVKIIAG
                     AIDGTTGPIVDVVNLPEYYDVRLPENVEYAHRTTLGSTVLIYVYEGSGSIEGSGSIVG
                     SGASGGSGGLEIGNRSLILFEDGRDIAIKSGAAGLKYLYVSGAPLREPIAWRGPIVMN
                     TREELRLAYEELDNGTFIKTNTRNRH"
     gene            complement(1621..2397)
                     /locus_tag="PLQ29_04790"
     CDS             complement(1621..2397)
                     /locus_tag="PLQ29_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG85995.1"
                     /translation="MKTKETRSRVMDDPVLDRLFTEAYRDALARVGGLEQEKRLADHV
                     EAYAELFERDLDARDLSDSERREMVAAFKRRLASVREERDALRRRRRGRALIGGLIAA
                     CVLATGGLYLASAQPFKAAAEIERELGLYYAKVDAGTGAYAKRYFALLAKHGSKLGSM
                     KAEDYGRSMRAELDEHFNEYLDRVVAGELQYADDAKAWAKLFPDKEEREERYRAVDNA
                     FQAGLGKAVGATWDTVKKETGSLFEEAGEAVKKLVDTLKD"
     gene            complement(2443..4146)
                     /locus_tag="PLQ29_04795"
     CDS             complement(2443..4146)
                     /locus_tag="PLQ29_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HPG85996.1"
                     /translation="MKNDGAFVSSECRALPALILCMTCMLVALTAVSCGRSKPVAAAG
                     AVASSSSADTATEDAAAAEAAEAAAIAAAEAAKAAALEASETAAVGQAEAARIAAIAV
                     PAPVVSGPAEGVYAVAVTVSASVALPEARLVYTLDGSTPSGRNGTPYTKPLEIAKSAT
                     VRFAAYVPGGNTSSIVSLEYAIGEVCAAPGGRGDGRRGAPFGSVSEAVTKARSLGIGL
                     VKLAAGASFMESVSLDAPITLSGGWAKGFSSRSGGYAVVIGLESDSSSKKAPAYAIRI
                     SGKAADAGVRIERVEFRGGAGSYSAGIVVDGGAAPVFSDCKASGGDGSYGYGAAVVGG
                     AAPRFDSCRLSGGDGATSYGLSVDSAKAVAAGSFLLAGSGAVGGYGLSATDAVIRVAS
                     SVLAGSPANVSYGAAFYNCRDSALESSTVVGGSGKDSAGVFISASDPSIVNCIIGSYG
                     SAKSYGIFDNYGSSAPSRLESVAFIGCSGGVYYDADSKRAYTAVDASGVPVDADGKAL
                     AKPKAAGCIVASFSLSATDGYATPASAAMPAGKALTGDAAVDIVGKARSEPWTIGAYE
                     L"
     gene            4396..>5145
                     /locus_tag="PLQ29_04800"
     CDS             4396..>5145
                     /locus_tag="PLQ29_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPG85997.1"
                     /translation="MYEPVDPKASFPSMEESVLAFWNDRDVFKRSISERDGSPEYVFY
                     DGPPFATGLPHFGHFVPGTIKDVIPRYKTMKGFKVERRFGWDCHGLPVENLIEKELGL
                     DSKTDIERFGVANFNEACRASVQRYVKEWRTIMTRAGRWVDFDNDYKTMDPDYMESIW
                     WVVKNLWDQGLVYEGHYILPYCPRCSTVLSNHELALGGYKDVHDPAITIKFKVLEAPA
                     GLEGLADGSTYLLAWTTTPWTLPSNLALCLGP"
BASE COUNT          955 a         1641 c         1675 g          874 t
ORIGIN      
        1 gcgcctgctc gccgacggca tgacgcccaa cccgctcctg acccgcatcg ggctgaacac
       61 cggcgacatg gtcgtcggca acatgggcac cgagcgcaag atggactaca ctatcatggg
      121 caacgcggtg aacctcgccg ccaggctcga gggcgtcaac aagcagtacg gcagctggat
      181 actgatgtca gacgcgacct accaggaggc cggagaggag ttcctggcca ggcgcctcga
      241 tcgggtcagg gtggtcggca tcaatacgcc ggtccagctc tgggagctgg tcggcctcaa
      301 ggccgatacg gacgaaggga cgctcgactt cctggcccgc ttcgaggaag cgcacaaaac
      361 cttcgacggg cgagactgga agaaggccgc gaggttgctg tcggccctgg ccgccgagcg
      421 cccggacgac ggtccgacga agtcgtatat aaagaagtgc gaagtcttca ccctgaagcc
      481 tccggcgtcc gactgggacg gggtgttcag cttgacggaa aaataagcga tcgctgacga
      541 gctcgcgctc cggcggcgcc ggatcgcgta ctcgcagcga aagccgcctg aaattgaagc
      601 ggtcgtgcgc ggtagcgctc gccgcatact ttcggggcgg gtggcgagga tgcttatacg
      661 gcttcgttga ggagtgaggt acatcatgca tgactatcgg gcagttcgct taattcgagg
      721 ggccaaggcg gtcgtcgagg gcgcgggagt acgcctctat agagccgtcg ggctgggcga
      781 tcctgaggag tgtgatccgt tcctcttgct cgacgatttc cgctcggacg atccgaggga
      841 ctatagcagg gggtttccgt ggcacccgca taggggcata gagacgataa cgtacgtgct
      901 caagggcacc gtggagcacg aggacagtat cggcaataag ggcgtgatcg ggccaggcga
      961 tgtccaatgg atgaccgcgg ggagcggcat cttccatcag gagatgccgc gcggtgacgg
     1021 cgatggctcg atgcacggct tccagctatg ggctaacttg ccggcgagcg ataagatgaa
     1081 agagccgagg tacaggggca tacgcggcga ggatatcccg aaggtaacga ctcctaacgg
     1141 cgtggtcgtc aagattatcg ccggcgctat cgacggcacg accgggccaa tcgtcgatgt
     1201 cgtcaactta ccggaatact acgatgtacg ccttccggag aatgtcgagt atgcgcatcg
     1261 gacgacactc gggagtaccg tccttatcta cgtgtacgag ggctcgggct ccatcgaggg
     1321 ctcgggctcc atcgtgggct cgggggcgag cggcggctcg ggtgggctcg agatagggaa
     1381 ccgttcgctc atcctcttcg aggacgggcg ggatattgcg atcaagagcg gggcggccgg
     1441 gctcaagtac ctgtacgttt ccggtgcgcc gctccgcgaa cccatcgcgt ggaggggccc
     1501 gatagtcatg aacaccaggg aagagctacg gctcgcgtac gaggagctag ataacgggac
     1561 gttcatcaag accaacacga ggaatcggca ctagagaacg tcggtatcgg gcggcatgcg
     1621 tcagtccttg agcgtgtcca cgagcttctt gaccgcttcg ccggcctcct cgaacaagga
     1681 gccggtctcc ttcttgacgg tgtcccaggt agcgccgacg gccttgccta ggccggcctg
     1741 gaaggcgttg tccacggcgc gatagcgttc ctcgcgctcc tctttatcgg ggaagagctt
     1801 ggcccaggcc ttggcgtcgt cggcgtactg gagctcgccc gcgactaccc ggtcgaggta
     1861 ctcgttgaaa tgctcgtcca gctccgcgcg catggagcgt ccgtagtcct cggccttcat
     1921 cgagcctagc ttggagccat gcttggcgag cagcgcgaag tagcgtttgg cgtaggcccc
     1981 ggtcccggcg tcgaccttgg cgtagtatag accgagctcg cgctcgatct ccgccgctgc
     2041 cttgaacggc tgggccgagg ccaggtagag gcctccggtg gccaggacgc aggccgctat
     2101 aaggccaccg atcaaggccc gcccgcgcct acgtctgcga agggcgtctc gctcctcccg
     2161 aacagaagcc aagcgacgct tgaaggcggc taccatctcg cgccgctccg agtcggatag
     2221 gtcgcgggcg tccaaatcac gctcgaaaag ttcggcgtac gcttcgacgt ggtcggccag
     2281 gcgcttctcc tgctcgaggc cgcctacccg cgccagggcg tcccgatacg cctccgtgaa
     2341 gagcctatcg aggacgggat cgtccatgac ccgtgacctg gtctctttcg tcttcatggc
     2401 actactatac cgacaaggcg ccgccgcgcc aacgcggccg gcctacagct cgtaggctcc
     2461 tatggtccag ggttcgctcc gggccttgcc gacgatgtcg acggcggcgt cgcccgttag
     2521 ggccttgccg gcgggcatgg cggccgaggc cggcgtcgcg tagccatccg tggcgctcag
     2581 ggagaacgac gcgacgatgc agcccgcggc cttaggcttg gccagggcct tgccgtcggc
     2641 gtccacgggg actccggacg catcgacggc ggtatacgcg cgttttgagt cggcgtcgta
     2701 gtagacgccg cccgagcagc caatgaaggc cacggactcg aggcgcgacg gggcggacga
     2761 gccatagtta tcgaaaatgc cgtaggactt ggcgctcccg taggagccaa tgatacagtt
     2821 gacgatggag ggatcggagg cggagatgaa gacgccagcc gagtccttgc cggagccgcc
     2881 gacgacggta ctggattcga gcgccgagtc cctgcaattg tagaacgcgg cgccgtagct
     2941 gacattggca gggctgccgg ccagcaccga gctggcgacg cgaatgacgg cgtcggtggc
     3001 tgacaggccg tagccaccga cggcacccga gccggcgagc aggaacgatc cggccgcgac
     3061 cgccttggcc gagtcgaccg ataggccgta ggaggtggcg ccgtcgccgc ccgacaggcg
     3121 gcacgagtcg aagcgcgggg cggccccgcc gactacggcc gcgccgtaac catagcttcc
     3181 gtcgcctccc gaggccttgc agtcgctgaa gaccggcgcc gcgcccccgt cgacgacaat
     3241 gcccgccgaa taggagccgg ctccgccgcg gaactcgacg cgctcgatcc gtacgcccgc
     3301 gtcggccgct tttcccgaga tccgaatggc gtaggctgga gctttcttgg acgacgagtc
     3361 cgactcgagg ccgataacga cggcatagcc gcccgagcgc gaagaaaagc ccttcgccca
     3421 gccgccggag agggttatgg gcgcgtcaag gctcacggac tccatgaacg aggctccagc
     3481 ggccagcttg acgagcccta tgccgagcga cctggccttg gtaacggcct cggagacgct
     3541 tccgaacggg gcgccgcgac gtccatcgcc cctgcctccg ggagcggcgc agacctcgcc
     3601 tatcgcgtac tcgagcgata cgatgctcga ggtattgccc cctggcacat aggccgcgaa
     3661 gcgtacggtc gcgctcttcg ctatctcgag cggcttcgta tagggcgtac cgttccggcc
     3721 gctcggggtc gatccgtcga gcgtgtagac gagcctggcc tcgggtagcg ccacggacgc
     3781 ggacaccgtc acggccaccg cgtaaacgcc ctcggccgga ccggagacta caggcgctgg
     3841 cacggctatc gccgcgatcc gcgcggcctc cgcctgcccg acggctgccg tctccgacgc
     3901 ttccaaggcc gcggcctttg ccgcctccgc ggccgctatc gccgcggcct ccgccgcttc
     3961 cgccgccgcg gcatcctcgg tggccgtatc ggccgaggat gatgaagcca cggccccagc
     4021 ggccgccacc ggcttcgatc gaccgcagga tacggcggtc agcgcgacga gcatacacgt
     4081 catgcatagg atgagggccg gaagcgctcg gcattccgac gatacgaagg ctccatcgtt
     4141 tttcatacgc tctccacgat accgccgccg ctaaaaagta ccgccttgac cgtacaagga
     4201 taggtcaagg cggctccgcg acgcgacgat gcatatagaa atataacact tggcaatact
     4261 attttcacgc aatcgctgaa atgactatat tcatatcgat agagtttgac tagggcgcga
     4321 ctggtgtcgt accgctggaa ctgacgcgcc gctttatggc ataatcggca gtcacatcgt
     4381 cccggaggag catcaatgta cgagcctgta gatccgaaag ccagtttccc gtcgatggag
     4441 gagtccgtcc tcgcgttctg gaacgatcgc gacgtcttca agcggtccat ctccgagcgg
     4501 gatggctctc ccgaatacgt attctatgac ggcccgccct tcgccaccgg cctcccccac
     4561 ttcggccatt tcgttccggg cacgatcaag gatgtcatcc cccgctacaa gacgatgaaa
     4621 ggcttcaagg tcgagcgccg cttcggctgg gactgccacg gtctgccggt cgagaacctc
     4681 attgagaagg agctcggcct cgactccaag accgacatcg agcgcttcgg cgtggccaac
     4741 ttcaatgaag cctgccgagc ctcggtacag cgctacgtca aggaatggcg caccatcatg
     4801 acccgcgccg gacgatgggt cgacttcgac aacgattata agacgatgga tcccgactat
     4861 atggaatcga tctggtgggt cgtcaagaat ctctgggacc agggcctcgt ctacgagggt
     4921 cactatatcc taccgtactg cccccgttgc tcgacggtgc tgtcgaacca cgagctggcg
     4981 ctcggcggtt acaaggacgt gcacgacccc gccatcacga tcaagttcaa ggtcctcgag
     5041 gcgccggccg ggctcgaagg gctcgccgac ggctctacct acctcctcgc gtggacgacg
     5101 actccatgga ccctgccgtc gaacctggcg ctctgcctcg gcccg
//
LOCUS       DAOPHV010000131         5123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_21691_length_5123_cov_4.051105, whole genome shotgun sequence.
ACCESSION   DAOPHV010000131 DAOPHV010000000
VERSION     DAOPHV010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21691_length_5123_cov_4.051105"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLQ29_04805"
     CDS             <1..639
                     /locus_tag="PLQ29_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPG85998.1"
                     /translation="RCLEMILAEGERQYGLASADAAGVAEGAERGVAARVGAVVEAAL
                     DAGERLLGIERGPGDSIERLYRIRQIGWDRVFLPPEEDPRNVSGMRRAVLDRRAGEAW
                     YAMRHMELADFAWYLRSGPPAADGGLTTGPIVEYAQNLWDFANRLAGGAISGRREVRP
                     KRAVVTASAPIDLSARLEDYRRDRKSAQAAIMDELKRSYTACIKEFINEERG"
     gene            623..1984
                     /locus_tag="PLQ29_04810"
     CDS             623..1984
                     /locus_tag="PLQ29_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG85999.1"
                     /translation="MKSAVETAGRLPVRTKVGFGIADLGGNLFFTAMGFWSMFYLTDG
                     VGIPAAMAGLAVMIGKIWDAVTDPMMGYISDRTDSKLGRRRPYIIFGALPLMATMVYF
                     FKVPAFTNPVALTVWATAALCLLNTAYTVVNVPYASLTPELTKDYNERTSLNGYRFAF
                     AAVGTMLGASIVQPIIGMAKTKAAGFLLVGLIFGAVMAVTSLITGFSVRERHREGEIP
                     HGFIKTYSSVFKNRPYLILLFTYALNLTGLNFLQGILAYYFKYIVGDEAMTTMAMVLL
                     IGVAMAFIPISVAIAKKVGKKIVYQLCFGTLTAVCAVIFFFGHRMPMPALLVVMAFGG
                     VGIGFGYVPPYAMLPDAIEVEAARSGKRNEGAYYGIWTLMSKLGTSAATALLGFVLAL
                     ARYVPDVEQAPEALFAIRLLVGPIPMVFLIGAAVLVQFYPLDEKTYEAMIAGSRREDG
                     SAV"
     gene            complement(1933..2457)
                     /locus_tag="PLQ29_04815"
     CDS             complement(1933..2457)
                     /locus_tag="PLQ29_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86000.1"
                     /translation="MKSLGNEPYLGIVSPLPSPLEQYEDQELRAEFRLALRRARLRST
                     LGAIIDGFCGLYERLGLGRPRVRPAPDAPATTPPATIGIDQVAGTIAPDGRLVAGPPR
                     LSRRDYAAWAVAYAAVREHGPSPIHGRYVDGGFYLDTGRSLAVRVEAARALGYEALQT
                     ADPSSRRDPAIMAS"
     gene            complement(2522..3529)
                     /locus_tag="PLQ29_04820"
     CDS             complement(2522..3529)
                     /locus_tag="PLQ29_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPG86001.1"
                     /translation="MKKPRLRSIDDLAKLAGVSKSTVSRALNDSPLVGVATKDKILAL
                     AKDYDFRPSVTARALSTRSSRTVAFVIDAYDPECGLHSPFSLEIMGGAATGLHELGYG
                     MLIVQADGARGDWAEEYLVSGRVDGFILMTYENKRRHVQALVDLEAPFTVWGSCDMDP
                     CCSVSTDNRLGGRLAGEHLVATGRRRIGFIGGSMDDSEVVQRLLGFSEAVAIDRSLHT
                     PGLEAFGDFSEPSGFVAMNELLDRDGSVDAVFAAGDMMAIGAIRALATRGRRVPDDVA
                     VVGYDDLFVSAFTTPSLTTISQHIADTGRTLASRLVANIERGVVESAVVPVELIKRDS
                     A"
     gene            3628..4818
                     /locus_tag="PLQ29_04825"
     CDS             3628..4818
                     /locus_tag="PLQ29_04825"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPG86002.1"
                     /translation="MPNHSSLAAVMFVELAGFSDILAASERTALSLLGEYRRLADPIV
                     AEHSGETVDATNSELLVVFSSAVAAVQCALHLVLALAPLSAEGRDDVIAEPRVGLHLG
                     EIWRDSGRVYGNGVNVAARVMQAAPAGAVYLTEDVFRQVSSKLDLNSRPVGAVSMKNI
                     DRPIALYELDAGRGFVEGVAADGASAAGGDRPCEPETASNGPAESGGNLSDDAAAIAA
                     GIASLKQLKNLKSLKDLGSLKALEGLKGLKALEKLEELDRLDKDGELDGLGESIARRV
                     EANLKGIPGVTARTDDGTLDVSFEIGSGKVAGAAGRQAVDPGAAADKARARIAAGLRK
                     MLMSFGLGAAFGYGYASTDQWLFLAGAAAFGLFPFISGAKRLISAASELRLIEKRRKR
                     GAGA"
     gene            complement(4879..>5123)
                     /locus_tag="PLQ29_04830"
     CDS             complement(4879..>5123)
                     /locus_tag="PLQ29_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPG86003.1"
                     /translation="LKRRIAKRGRDFEASISDEYLAGLNRLYEEWIDGFDLAPVLVVP
                     GDRLDFVAESKDLDSIVRTIEGRLRDKQGSLFPVGM"
BASE COUNT          870 a         1608 c         1731 g          914 t
ORIGIN      
        1 cgttgcctcg agatgatcct ggccgagggc gagcgtcagt acggcctcgc gtcggccgac
       61 gccgctggcg tcgcggaagg cgccgagcgc ggagtggcgg ccagggtcgg cgccgtggtc
      121 gaggcggccc tggatgccgg ggaacggctt ctcggtatag agcgggggcc tggcgactcg
      181 atcgagcggc tgtaccgtat caggcagata ggctgggatc gcgtgttcct gccgccggaa
      241 gaggatcctc ggaacgtttc gggcatgcgg agagctgtac tggaccggag agccggagag
      301 gcttggtacg cgatgcggca tatggagctg gccgacttcg cctggtacct gcgctcgggg
      361 cctcccgccg ccgacggcgg cctgacgaca gggcctatcg tcgagtacgc ccagaacctc
      421 tgggacttcg cgaatcggct agccggcggc gcgatatccg ggcggcgcga ggtgcggccg
      481 aagcgggccg tcgtgaccgc gtccgcgccg atagacctga gcgcgcggct cgaagattat
      541 aggcgcgacc ggaagagcgc ccaggcggcc atcatggacg agcttaaacg atcgtatacg
      601 gcatgcataa aggagttcat aaatgaagag cgcggttgaa acggccgggc gcttgcccgt
      661 ccgcacgaaa gtaggcttcg gcattgccga cctgggcggt aacctgttct ttaccgcgat
      721 ggggttctgg tccatgttct acttgaccga cggcgtcggg atcccggccg cgatggccgg
      781 actggccgtg atgatcggca agatctggga cgcggtgacc gacccgatga tgggttatat
      841 ctccgaccgg acggattcca agttgggccg gcgccggccg tacatcatct tcggcgcctt
      901 gccgctgatg gcgacgatgg tctatttctt caaggtaccg gccttcacga acccggtcgc
      961 gctgaccgtc tgggcgaccg cggcgctctg cctcctcaat accgcctaca cggtcgtcaa
     1021 cgtgccctac gcgtcgctga cgccggagct gaccaaggac tataacgagc ggaccagtct
     1081 gaacggctac cgcttcgcct tcgccgccgt cggcacgatg ctcggcgcga gtatcgtcca
     1141 gccgatcatc ggcatggcca agacgaaggc ggcaggcttc ctgctcgtcg gcctgatctt
     1201 cggcgcggtg atggccgtga cttcgctgat cactggcttc tcggtgcgcg agcggcatcg
     1261 cgagggagag ataccgcacg gcttcatcaa gacctactcg tcggtattca agaaccggcc
     1321 gtacctgatt ctcctgttca cctacgcgct caacctgacg ggcctcaact tcctgcaggg
     1381 catcctggcc tattacttca agtacatcgt cggggacgag gcgatgacga cgatggccat
     1441 ggtgctactg atcggcgtgg cgatggcctt tatcccgata tcggtagcca tcgccaagaa
     1501 ggtcggtaag aagatcgtct accagctgtg cttcggcacg ctgacggccg tctgcgcggt
     1561 catcttcttc ttcggccacc gtatgccgat gccggccctg ctcgtggtga tggctttcgg
     1621 cggcgtgggc ataggcttcg gctacgtgcc gccctacgcg atgctgccgg acgccatcga
     1681 ggtggaggcc gcgaggagcg gcaagcgcaa cgagggcgcc tactatggca tctggaccct
     1741 gatgtcgaag ctcggtacct cggcggcgac ggccctgctc gggttcgtgc tggccctggc
     1801 ccgctacgta ccggacgtgg agcaggcgcc agaggcccta ttcgccatcc ggttgctcgt
     1861 cggccctatc ccgatggtgt tcctgatcgg cgccgccgtg ctcgtgcagt tctaccccct
     1921 cgatgagaag acctacgagg ccatgatcgc cggatctcgg cgcgaggacg ggtcggcggt
     1981 ctgaagcgct tcatagccga gggccctggc ggcttctacc ctgacggcga ggctcctgcc
     2041 agtgtccagg tagaagccac cgtccacgta gcggccgtgt atcggggagg ggccgtgctc
     2101 ccgtacggcg gcgtaggcga cggcccaggc cgcgtagtcg cgtcgcgata gcctcggcgg
     2161 cccggccacc aggcggccgt cgggggcgat cgtgccggct acctggtcta taccgatcgt
     2221 cgccggcggg gtggtggccg gcgcgtcggg ggccgggcgc accctaggcc ggccgaggcc
     2281 caggcgctca tagagaccgc agaagccgtc gatgatggcc cccagcgtcg aacgtagcct
     2341 ggcccgcctg agcgccagac ggaattctgc ccgaagctcc tgatcctcgt actgctcgag
     2401 cggtgaaggt agcggcgata ctatccccag gtatggttcg tttcctagcg atttcatata
     2461 tacctccttc tcattgtgca accggttgca aaaccgagaa aaaaaagatg caggcatcag
     2521 atcaggccga atcgcgtttg atcagctcca ctggaacgac ggcgctctcg acgacgccac
     2581 gctcgatgtt cgccacgagg cggcttgcga gggtacgccc agtatctgct atatgctggc
     2641 ttatcgtcgt cagcgacggc gtcgtgaacg ccgagacgaa caggtcgtcg tagccgacga
     2701 cggcgacgtc gtcgggcacg cgcctgcccc tggtcgccag ggcgcgtatc gcgccgatgg
     2761 ccatcatgtc tccggcggcg aacacggcgt ctaccgaacc gtctcggtca aggagctcgt
     2821 tcatcgctac gaagccggac ggctctgaaa aatcgccgaa cgcctcgagc ccgggcgtgt
     2881 ggagggacct gtctatggcg acggcctcgg agaagccgag gagccgctgc accacctcgc
     2941 tatcgtccat cgacccgccg atgaagccga tgcgacggcg accggtggcg acgaggtgct
     3001 cgcctgccag gcgtcccccg aggcggttgt cggtcgagac ggagcagcac ggatccatgt
     3061 cgcaggaacc ccagacggtg aatggggcct cgaggtcgac gagagcctgc acgtgtcgcc
     3121 gcttattctc gtaggtcatt aggatgaagc cgtctactcg gcctgagacc aggtactcct
     3181 cggcccagtc gcctcgcgcc ccgtcagcct gaacgatcaa catgccgtaa cccagctcgt
     3241 ggaggcccgt ggcggctcct cccatgatct ccaggctgaa cggcgagtgg aggccgcatt
     3301 cggggtcata ggcgtctatc acgaaggcta ccgtacggct ggagcgcgtc gaaagggcgc
     3361 gggcggtgac gctcggtcta aaatcgtagt ccttggcgag cgcgaggatc ttatccttcg
     3421 tggcgacgcc gacgagaggg ctgtcgttga gcgctctcga gacagtggac ttggagacgc
     3481 cagcaagctt ggcgaggtcg tctatcgacc taagtcgtgg tttcttcata ttgcaaccgg
     3541 ttgcaatata tgattgcccc gactcatctg tcaagtggcg agcgatgtca cttgccaaca
     3601 acacgcccgg ctctttacat ttatgtgatg ccgaaccata gctcgctggc cgccgtgatg
     3661 ttcgtcgaac ttgccggttt ctccgatatc ctggccgcga gcgagcgcac ggccctgtcg
     3721 ttgttgggcg agtatcggcg cctagccgat cccatcgtcg ccgagcattc gggcgagaca
     3781 gtcgacgcga cgaattccga gctactcgtc gtgttctcga gcgcggtggc ggcggtacag
     3841 tgcgccctgc acctggttct ggcgctggcg ccgctgtcgg cggaaggtcg ggatgatgtg
     3901 atcgccgagc ctcgcgtcgg cctgcacctt ggagagatat ggcgcgattc cggccgcgtc
     3961 tatggaaatg gcgtcaacgt ggccgcccgc gtgatgcagg ccgccccggc cggcgccgtc
     4021 tacctgacgg aggacgtgtt ccgccaggta tcgagcaagc tcgacttgaa ctcgaggccc
     4081 gttggcgccg tatcgatgaa gaatatcgat aggcctatag cgctctacga gctcgacgcc
     4141 gggcgcggct tcgtcgaagg cgttgcggcc gatggcgcgt cggccgcagg cggagatcgg
     4201 ccatgcgaac cagaaacggc ttcgaacggg cccgccgaaa gcggtgggaa cctctccgac
     4261 gacgcggcgg cgatcgccgc cggtatcgcg agcctcaaac agctcaagaa cctcaagagc
     4321 ctaaaggacc tgggctcgct caaagccttg gaaggcctca agggactcaa ggctctggag
     4381 aagctcgagg agctcgaccg gctcgacaag gacggcgagt tggacggcct cggcgagagc
     4441 atagcccgta gggtcgaagc caacctgaaa gggattcctg gcgtgacggc caggacggac
     4501 gacggtacgc tggacgtctc gttcgagatc ggttcaggga aggtcgccgg cgcggccggt
     4561 cgccaagccg tggatcccgg ggccgcggcc gacaaggcta gggcgaggat agccgccggc
     4621 ctgcggaaaa tgctcatgag cttcggcctc ggagccgcgt tcggctacgg ctacgcgtcg
     4681 accgaccagt ggctgttcct ggcgggggcg gcagccttcg ggctcttccc gttcatctcc
     4741 ggcgccaagc gcctgatctc ggccgcctcg gagctacgtc tgatcgagaa gcgccggaag
     4801 cgcggcgccg gggcctagcg ctcggcgcgg gggcctagcg cgcggcgcgg gggcctagcg
     4861 cgcggcgtag gatgccggtt acatgcctac cgggaatagg ctgccctgct tgtcgcgcag
     4921 tcgaccttct atagtgcgca cgatcgaatc gaggtccttc gactcggcga cgaagtccag
     4981 ccggtcgccc ggcacgacga gcaccggcgc gaggtcaaag ccgtctatcc attcctcgta
     5041 aagccggttg agcccagcca ggtactcgtc ggatatgctc gcctcgaagt cgcggccgcg
     5101 cttggctatg cgcctcttga gcg
//
LOCUS       DAOPHV010000132         5117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_21721_length_5117_cov_4.560253, whole genome shotgun sequence.
ACCESSION   DAOPHV010000132 DAOPHV010000000
VERSION     DAOPHV010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21721_length_5117_cov_4.560253"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1002)
                     /locus_tag="PLQ29_04835"
     CDS             complement(67..1002)
                     /locus_tag="PLQ29_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HPG86004.1"
                     /translation="MIAPDLRDDELRRRLDGLAPDGLTIFTLEGDSIRGALVSATTMV
                     AEMRAAHGLGILEAEVLGRAYVAAALLSATIKGDDRLRIQVDGNGPAGGFSVECSANG
                     DVRGRLYNAPFELDETPASLDTAPLVGSGTISLTRFMAGKTEPVTGSAALRSGRLAED
                     LAWFYHISEQTRSSFTIGLHFDAEGRVAGAGGLFLQALPGAPEEALDRVERLVYGIAP
                     LGETFASGATRSDVCLRSFPFFDFNHLDDRPVRFRCSCSKERLGSYLASLPGGELDDM
                     AANGPFPVELTCHNCGSTYGFSRDELAAMAGRRHG"
     gene            1069..2535
                     /locus_tag="PLQ29_04840"
     CDS             1069..2535
                     /locus_tag="PLQ29_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86005.1"
                     /translation="MGYVELGYCILVLAVALDCAIARAAPYTSAPLAARRPLPFTRFD
                     IGMLAAFAAFLCFIVTDSLDWRLDAAAVAMFALWSVSFAASTRRRSRAGADETRGAAI
                     GRRVAGSAGALAAIFSLAAVSVFPIRDLEPPTGPHAVGTASFVVVDGSRTGVYLDAPG
                     TPRRVMAQAWYPAAPGAEDYPRARWIPDEALERAYAAYAGLPALTMSHLSRIRANGRV
                     EAPALGGSPAEDGSFPVVILSHGWTGSRTMHADLAEELASRGIVAIAVDHPYGALAVA
                     FPDGEVKNLYRGALPSRSDGVEAFDRASTFLEETYAADIAAVVGVVRGGRAVPGLEGR
                     LDGGRIALVGHSTGGGAALLAAMIAETAAESAAVSAATTTESGVVGFLGVVGLDAWVE
                     PIAARLDEGLATPQLHVGSASWAGGRNAELLGRLRAASGEWSETRIPGSGHADFAMLR
                     YVTSAGSVTGLAGERDGFAFAADVTPLVCDWLEARLSR"
     gene            2748..3161
                     /locus_tag="PLQ29_04845"
     CDS             2748..3161
                     /locus_tag="PLQ29_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86006.1"
                     /translation="MSVPCDRQLFRLALASVMTASMVLALGAQDVRVRVAPSLLVPLA
                     DTGTYGVGGGGSFVMDMDLLGFLAPYAGGDLSYVSMTADDFDGSLTLATGGAGLGVFL
                     FPLPRLKLAASGGSGVYVGSYTDPERESAMVGNVF"
     gene            3414..4838
                     /locus_tag="PLQ29_04850"
     CDS             3414..4838
                     /locus_tag="PLQ29_04850"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG86007.1"
                     /translation="MTIKNAESAEIRDVEVWFDAEGYTTGPALCASFPYLRKGAIASA
                     PLVAAFSDQVMTITENVRVRGEVRVAYTLLGAPRVARGETTLSIMNRNALTWSDARIL
                     ASFVSPNDPAVLDSSKFLAGVVRSKARMELDSNLQYALGIFEGLRLSGIAYAADPQTP
                     YAAMRARPDQVDYIQYPYQTLAYRGGDSDDIAALYAAELESIGVPAALVLLDDEVLVA
                     FRMSGGESTTRGAFSDAGDFIFVDGEAWAPIRVAMLREGFLRAWSDGSAVVKKYPEAR
                     DRFFRLTDAWRRFPPAGVPGIAASTRKPSEDQVRAAFDNAVSLVVAKEVAPRAERMRL
                     SFGSGGGTGKQHNSLGVLYARYGMYAEALTEFQAAAALGYTAVAVNIGNVAFLTGDYA
                     TAAAWFDKAAKDRPEDVAAIIGLARSLYELDRYDEADALFRKATNYMPELAERYGYLS
                     ARLTGTTARASAVMDRGGGMLWDE"
     gene            4876..>5117
                     /locus_tag="PLQ29_04855"
     CDS             4876..>5117
                     /locus_tag="PLQ29_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86008.1"
                     /translation="MKNRKGTGFLLAAVGSVAGVGAQGLPETGGQALDDARIGTPLAD
                     RFGVSAYADGWFPRALDGASAIEYGLGGTAELSFSAA"
BASE COUNT          765 a         1794 c         1731 g          827 t
ORIGIN      
        1 ccgcgggaca cgttacccgt gccgtcgacc ggccatcgcc gctagctcgt cccgccgcgg
       61 gacacgttac ccgtgccgtc gaccggccat cgccgctagc tcgtcccgcg agaacccgta
      121 tgtcgagccg cagttgtgac aggtcagctc caccgggaac ggaccgttcg cggccatgtc
      181 gtcgagctcg ccgcccggca gcgacgccag atacgagccg aggcgctcct tggaacagga
      241 gcagcgaaag cggaccggcc ggtcatccag gtggttgaag tcgaagaacg gaaaggagcg
      301 caggcagaca tcggaacggg tcgcgccgga cgcgaaggtc tcgccgagcg gcgcgatgcc
      361 gtagacgagg cgctcgacgc ggtccagcgc ctcctcgggg gcgccgggca gggcctgcag
      421 gaagaggccg ccggcgccgg ccacccggcc ctcggcgtcg aagtgaaggc cgatggtaaa
      481 cgagctgcgc gtctgctccg agatatggta gaaccaggcc aggtcctcgg ccaggcggcc
      541 ggagcgtagc gcggcgctcc cggtgaccgg ctccgtcttg ccggccatga aacgggtcag
      601 cgagatggtg cccgacccga cgagcggcgc ggtatccagg ctggccggcg tctcgtccag
      661 ctcgaagggc gcgttataga gtcggccgcg cacgtcgccg ttcgcggagc actccaccga
      721 gaagccgccg gccgggccgt tgccgtccac ctggatacgc agacggtcgt cgcccttgat
      781 cgtggccgac aggagggcgg ccgccacgta ggcccggccg aggacctcgg cctcgaggat
      841 accgaggcca tgggcggcgc gcatctcggc caccatcgtc gtcgccgata ccagggcgcc
      901 gcgtatcgaa tcgccctcga gcgtgaatat ggtcaagccg tcgggcgcga ggccatcgag
      961 cctccgccgc agctcgtcgt cgcgcaggtc gggtgctatc atcgtatcct ccgtagacga
     1021 tacagtatat cgaaaacagt cgtctcgggc ctacgtgaaa ggataaccat gggctacgtg
     1081 gaactgggct actgcatcct cgtcctcgcc gtcgcgctcg actgcgcgat cgcccgagcc
     1141 gcgccgtaca cgagcgctcc gctcgccgct cgacgccccc tgccgttcac ccgcttcgac
     1201 atcggcatgc tcgcggcctt cgccgctttc ctatgcttca tcgttacgga tagcctcgac
     1261 tggcgactcg acgccgccgc cgtggcgatg ttcgcgctct ggtccgtctc gttcgccgcg
     1321 tcgacccgca ggcgctcgag ggctggcgcg gacgagacgc gcggggccgc tataggccgc
     1381 cgcgtcgcag gctcggccgg cgcgctggcc gccatcttct cgctggcggc ggtctcggtc
     1441 ttcccgatcc gcgacctcga gccgccgacc ggacctcacg cggtcggcac ggccagcttc
     1501 gtcgtggtcg acggctccag gaccggcgtc tacctggacg cgccgggtac gccgcgccgc
     1561 gtgatggccc aggcctggta cccggccgct ccgggagccg aggactatcc gcgcgccagg
     1621 tggatacccg acgaagcgct cgagcgggcc tatgccgcgt acgcgggact gccggcctta
     1681 accatgtctc acctgagccg tatccgcgcc aacggccgcg tcgaggctcc cgccctcggc
     1741 gggtcgccag ccgaggacgg gagcttcccg gtcgtcatcc tgtcgcatgg ctggaccggc
     1801 tcgcgcacga tgcacgccga cctggccgag gaactggcca gccggggcat cgtcgcgatc
     1861 gccgtcgacc atccctacgg cgcgctcgcc gtcgccttcc cggacggcga ggtcaagaac
     1921 ctgtaccgcg gcgctctgcc gtcgcggtca gacggcgtgg aggccttcga ccgcgcttca
     1981 accttcctcg aggagaccta cgcggccgat atagccgccg tcgtcggagt cgtccgcggc
     2041 ggccgcgccg ttccggggct cgaaggccgc ctggacggcg ggcgcatcgc cctggtcggc
     2101 cactccacgg gaggcggcgc ggccctgctc gccgcgatga tcgccgaaac ggcggccgaa
     2161 tcggcggccg tttcggcggc gacgacgacc gagtcgggcg tagtcggctt cctgggcgtg
     2221 gtcggcctcg acgcctgggt cgagcccata gccgcgcggc tcgacgaagg cctggccacg
     2281 ccacagctcc acgtcggtag cgcctcctgg gccggtggac gcaacgccga gctgctcggg
     2341 cggctgcgcg cggccagcgg cgagtggtcc gagaccagga tacccggctc cggccacgcc
     2401 gacttcgcga tgctacgata cgtgacctcg gccggctcgg tgacgggcct ggccggcgag
     2461 cgagacggct tcgccttcgc cgccgacgtc acgccgctcg tctgcgactg gcttgaggcg
     2521 aggctgagcc gctagcgcgc tctgtatgaa gccgcacgcc gtaaacgccg gcgtcgcggc
     2581 gactcgacaa ttgcgtgcat caaatttatc gttttctggt aaaatttgaa tgtatgctac
     2641 gatatctgat gatatactga tacataacag taatgtgtcg cctcgcgtag catccagcct
     2701 gttcttccgc ctcgacgagt ttcgtatatg tcctgaaagg agccgcgatg agcgttcctt
     2761 gtgataggca gctattccgc ctcgccctgg cgtccgtgat gacggcgtcc atggtcctcg
     2821 ccctcggcgc ccaagacgtc cgcgtccgcg tggccccgag cctgctcgtc ccgctcgctg
     2881 acaccggcac ctacggtgtt ggtggcggcg ggtccttcgt catggacatg gatctgctcg
     2941 gcttcctggc accctacgcc ggaggcgacc ttagctacgt gtccatgacg gctgacgact
     3001 tcgacgggtc gctcacgctg gccaccggcg gagcggggct cggcgtcttt ctattcccgt
     3061 tgcccaggct caagctggcc gcgtccggag gctcgggcgt ctacgtcggt tcctacaccg
     3121 atcccgagcg cgagtccgcg atggtcggca acgtgttcta gcgggccggg gccgagctcg
     3181 gctaccgttt cagtccgtcg ttcacgctat cggccggggc ctcgtacctg gacctgctcc
     3241 agaaggacga cggcgtcgtc gactcgttct ataagggcct ggcgctatcg ctggtagcgg
     3301 acattggctt cgagtcgaag aacgccgagg gccgggtcgc cctacagtca tcagagtcgt
     3361 cgcccgtcta cccgattgta tccgtcgatt acgccaagga gcccttcggc tccgtgacga
     3421 ttaagaacgc cgagagcgcc gagatccgcg acgtggaggt ctggttcgat gccgagggct
     3481 acacgaccgg tccggcgctc tgcgccagct tcccgtacct gcgcaagggc gccatcgcca
     3541 gcgctccgct cgtggccgcc ttctccgacc aggtcatgac gatcaccgag aatgtacgcg
     3601 tgcgcggcga ggtacgcgtc gcctacaccc tgctcggcgc tccccgcgtc gcccgcggcg
     3661 agacgactct gtcaatcatg aaccgcaacg cgctcacctg gtcggacgcc aggatcctcg
     3721 cctcgttcgt cagcccgaac gacccggccg tgctcgactc gagcaagttc ctggccggcg
     3781 tcgtacgctc caaagccagg atggaactgg actcgaacct gcagtacgcc ctcggcatct
     3841 tcgaggggct caggctctcc ggaatcgcct acgccgccga cccgcagacg ccctacgccg
     3901 cgatgcgcgc caggcccgac caggtcgact atatccagta cccgtaccag accctcgcct
     3961 accgcggcgg cgactccgac gacatcgcgg cgctctacgc ggcggagctc gagtcgatag
     4021 gcgtaccggc cgcgctggtg ctactcgacg acgaggtgct cgtcgcgttc aggatgtcgg
     4081 gcggcgagtc gacgaccagg ggcgcgttct ccgacgcggg cgacttcatc ttcgtcgacg
     4141 gcgaggcctg ggcgccgata cgcgtggcga tgctgcgcga aggcttcctg cgcgcctgga
     4201 gcgacggctc ggccgtcgtc aagaagtatc ccgaggctcg cgatcggttc ttccggttga
     4261 ccgacgcttg gcggcgcttc ccgccggccg gcgtgcccgg catcgccgcg tccacgagga
     4321 agccgtccga ggaccaggtc cgcgccgcct tcgacaacgc ggtgtcgctc gtcgtggcca
     4381 aggaggtcgc gccgcgcgcc gagcgcatgc gcctatcgtt cggctcgggc gggggcacag
     4441 gcaagcagca caactcgctc ggcgtgctct acgcgcgcta cggcatgtac gccgaggccc
     4501 tgacggaatt ccaggcggcc gccgcgctcg gctacacggc cgtcgcggtc aatatcggca
     4561 acgtcgcgtt cctgaccggc gattacgcga ccgccgcggc ctggttcgac aaggccgcca
     4621 aggacagacc cgaggacgtc gctgccatca taggtctcgc gcgctcgctc tacgagctcg
     4681 accgctacga cgaggccgac gcgcttttcc gcaaggcgac gaactacatg ccggagctgg
     4741 ccgagcgcta tggctatcta tcggcgaggc tgaccggcac cacggccagg gcgtcggccg
     4801 tgatggacag aggcggaggc atgctctggg atgaatgagc ccgggggaga ccttcgggcg
     4861 taagggtggc tattcgtgaa aaatcgcaag ggaaccggat tcctgctcgc ggccgtcggg
     4921 agcgtcgcgg gagtcggggc gcagggtttg cccgagacgg gcggacaagc gctggatgac
     4981 gctcggatcg gtacgccgct ggcggaccgc ttcggcgtgt cggcctacgc cgatggctgg
     5041 ttcccccgcg ccttggacgg cgcgtcggcc atcgagtacg gactcggtgg caccgccgag
     5101 ctatcgttct cggcagc
//
LOCUS       DAOPHV010000133         5110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_21755_length_5110_cov_3.876558, whole genome shotgun sequence.
ACCESSION   DAOPHV010000133 DAOPHV010000000
VERSION     DAOPHV010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21755_length_5110_cov_3.876558"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1854
                     /locus_tag="PLQ29_04860"
     CDS             <1..1854
                     /locus_tag="PLQ29_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86009.1"
                     /translation="PASASPAVADPAEADEEFESLDIDLQFDDTIPSAESEEATDTFS
                     SEDDILGSSSEFESIDLDSIGLDEPKHKPAPVAVREAPIASEPTDDSEGISLDSFIDS
                     DDDASIGIIPDLEIESVSIGSGESGFDDIQAVSDDLASSAPSSDLLRQIASELSSIKQ
                     ELVSLRDQLSSIKAAGASVGEAASAEGVEDELADASGGFFDDEDDDTIALTGDELDNI
                     LNTADFTEETVSEEPEDEAPELSIPDDVELLPEDGVYAEASEPGIETIELAEEATSFA
                     DADLSGEGIEAVEPIDGVTPIIPGPEDTSFLDDDTEARDFQELEGMPIEDVPLVEPSE
                     TDLDIDLAPAFEGDDEDLPVIEAADDESELVLDIDAGDEPVVSTVDSFDEPIDEVEDA
                     MDLEEVIEPEELGGLELHAEKSTEPVEELLPSDDVLEELTLDDFGDDSVDELVVEAPL
                     ETPQASDLSYHPDEIATSLDDSLFVGLEQDEVAEELELIDDDTLADEAESAVEEIVAD
                     EPLADEADSAVEEIVGDEPLARFEPELAVDEAPVTKAPPRAEAAPTTHSRAEAEATAD
                     STPDKLKRDVKSVLVYLDQLLASLPEEKIEEFASSEYYDTYKRLFDDLGLL"
     gene            2297..2710
                     /locus_tag="PLQ29_04865"
     CDS             2297..2710
                     /locus_tag="PLQ29_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86010.1"
                     /translation="MHQVEPASAEELIRIGSAKQFSFVSLAMLRTLELSSAAVRRCPL
                     SSPSSLNEELMACVSSILGTSGRAFGLADGSCLMAFYTHNAGDAELVAAQVARTLRRA
                     LAVEPSWPLATGPYTSLKLSGPDAEAKIRSFIDGL"
     gene            2700..4271
                     /locus_tag="PLQ29_04870"
     CDS             2700..4271
                     /locus_tag="PLQ29_04870"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HPG86011.1"
                     /translation="MGCEARIGSPTFPEPDPTSFHSRYAPEREAARHVRACIGSKRPA
                     IVYIIGGGMGYIHEAVSELLPRARLVSLQPCDLFHAHELGKPAITWRPGVAEPLESVL
                     ARSLADGRMAGGVSVIEWTPVTRRFTAQSERIRLALRDALESASSDAATSAYWSAGWL
                     RNAARFAAAARRQVRIRNGDGPIVIACAGPSLMRFLPELSRRRDDIALWALASAVPAL
                     VAAGLTPDLAVATDPGFWNALHLRTITELGIPIALSPSAFATEDALYRCPILALDTGL
                     PFERAAVEAAGFEDHRCSASGSAAGTALSLALACTGGPVTLAGYDLAAFGHEDHCRPY
                     PFDSLDLALASRTRPVTSAGASRTFDGYQAAVGAWRLSRAFSAYALSIRPPLGDSARA
                     FRLSDSPVPTPVPRIARLDDLPPTRGEPPVVTPLDDGSRDSSLDSAAIRREGYLDRVS
                     SSLEASLAGATEALASSVPMPFDAMTVYRALAPEATAPLVASMARGEATERELLAVHS
                     AAEQALFSMRGGCDG"
     gene            4264..>5110
                     /locus_tag="PLQ29_04875"
     CDS             4264..>5110
                     /locus_tag="PLQ29_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HPG86012.1"
                     /translation="MAEANLAHEPLDAQLFERNMLAVASCHGPVCADRIRSARSDPRV
                     GVQTSRSGHPVPFVGLGSRQALMHSRFDPVREAGRLASSYSKAAFYVFLGLGAGYEAA
                     AMLEADLVTRGLIVEYGATTLRAVLERVDLTHVLGDRRLRLLVDPEEAELNEVIAGCY
                     VPAVTGDIVSVPLRGRIDASPEPFHRAADAIRRSIDAISDDYSVQSFFGKRWFSNIVR
                     NLELSNAPTPPVGPIRSAYITAAGPSLERYAEEIRLRPKDAFLIATDTSLRALLGRGI
                     RPDAVI"
BASE COUNT          927 a         1709 c         1549 g          925 t
ORIGIN      
        1 ccagcttcgg ccagcccagc tgtagccgat ccggccgaag ctgacgagga gttcgaatcc
       61 ctcgatatcg accttcagtt cgacgacacc atcccatcgg cggaatcgga ggaggcgacc
      121 gataccttct cctccgaaga cgacatcctg ggctcgagct cggaattcga atcgatagat
      181 ctcgattcca taggcctcga cgaacccaag cacaagcccg ctcccgtagc cgttcgcgag
      241 gcaccgatcg cgagcgagcc gaccgacgat tccgagggca tatcgctcga ctccttcata
      301 gattccgacg acgacgcgag catcgggatc atccccgacc tcgagataga gagcgtatcc
      361 atcggttccg gcgaatctgg cttcgacgac atccaggccg tatcggacga cctcgcctcc
      421 tcggctccgt cctccgacct gctcaggcag atcgctagcg aactgtcatc gattaagcag
      481 gagctcgtga gccttcgcga ccagctgagc agcatcaaag cggctggagc ctccgtcggt
      541 gaagcggcct ccgcggaagg cgtcgaggat gagctagccg atgcatccgg tggcttcttc
      601 gacgacgagg atgacgacac gatagccttg accggcgacg agcttgacaa catcctcaat
      661 acggccgact tcaccgagga gaccgtttcg gaggaacccg aggatgaggc tcccgaactg
      721 tcgatacccg acgacgtcga gctattgcct gaggacggcg tctacgccga ggcctcggag
      781 cccgggatcg aaaccatcga gctagcggag gaagctacga gcttcgcgga cgctgacctt
      841 tcgggcgagg ggatcgaagc cgtcgagccc atcgatggag ttacgccgat catccccggg
      901 cccgaggaca cgagcttcct ggacgacgat accgaggctc gggatttcca ggagctcgag
      961 ggcatgccca tagaggacgt tcctctcgtc gaaccgagcg agactgatct cgatatcgac
     1021 ctcgctcccg ccttcgaagg ggacgacgag gacctgccgg tcatagaggc cgccgacgac
     1081 gaatcggagc tggtactcga catagacgct ggagacgaac ctgtcgtctc gacggtggac
     1141 tcgttcgacg aacccatcga cgaggtagag gacgcaatgg acctcgagga ggtcatcgag
     1201 cctgaagagc taggcgggct cgagctccat gccgagaagt cgaccgagcc ggtcgaggag
     1261 ctgctaccaa gcgatgacgt cctcgaggaa ctcacgctcg atgatttcgg cgacgattcc
     1321 gtcgatgagc tcgtcgtgga ggctccgctc gaaacgcccc aggcatccga cctgtcctac
     1381 catccggatg aaatagccac gagtctcgac gattccctat tcgtaggcct ggaacaggat
     1441 gaggtagccg aggagctcga gctaatcgat gacgatacgc tcgccgacga agccgagagt
     1501 gcagtcgagg agatcgtcgc ggatgagccg ctcgccgacg aagccgatag tgcagtcgag
     1561 gagatcgtcg gggatgagcc gctcgccagg ttcgagcccg agctagccgt cgatgaagcg
     1621 cctgtcacca aggctccgcc gcgcgccgag gcggctccca caacgcactc cagagctgaa
     1681 gcggaagcca cggccgattc gacgcccgac aagctgaagc gcgacgttaa gtcggtgctg
     1741 gtctatcttg accagctact cgcctcgttg ccggaagaga aaatcgagga gttcgcctcg
     1801 agcgagtatt acgatacgta caaacggcta ttcgacgatc tggggttgct gtgatgggac
     1861 ttttagagaa ggcggcgagc cgttcagacg cgctcgccgc taggaatgat ggccttctgg
     1921 ccataagcca aaaaaaaaag tctccgcggt atatcacgag tctatcgata ggctcctcgg
     1981 gggcatagat aaccgtcatc taggtctccg gctagcgatc gaggccttag ccgccgcggg
     2041 ctatccttgg gtcggttatt actcgaagcg ggtcgagtct gtagtccgct tcgccgcgta
     2101 cggcgccgat ctacccgact ccatgtccgc agaatcgcta ccctccctca aagtaggtgg
     2161 cgagcccgtc gagacgctac gccttgaggg cgaccagttc attataacgc cctcctcgac
     2221 atctctatcc gactcggtac aaaataccct caggcgcata ggacggacgt cgcccgccgc
     2281 tacgttcagg atagccatgc atcaggtcga gcccgcgtca gccgaggagc tcatcaggat
     2341 agggagtgcc aaacagttct ccttcgtgtc gctggccatg ctgaggaccc tggagctatc
     2401 aagcgccgcc gttcggcgct gccccctatc gtcgccttct agcctgaatg aagagctgat
     2461 ggcctgcgta tcgtcgatac tcggtacgag cgggcgagcc ttcggcctgg cggacggctc
     2521 gtgcctaatg gccttttata cgcataacgc cggcgacgcc gagctggtcg cggcccaggt
     2581 cgctcgtacg ctgaggcgcg cgctggccgt agagccttct tggccgttgg ctaccggacc
     2641 atatacatcg ttgaagctat ctggccccga tgccgaagcc aagatccgct ccttcatcga
     2701 tgggctgtga ggcgcgtatc gggagcccga cgttccctga gcccgatcca acctcgttcc
     2761 actcccgata cgccccggag cgggaggccg ccaggcacgt tcgcgcctgt atcggctcga
     2821 aacgcccggc catcgtctac atcattggcg gcggcatggg ctatatccac gaggccgtat
     2881 cggaactcct ccctagggcg cgattagtct cgcttcagcc ctgcgacctc ttccatgcac
     2941 atgagctcgg caagcccgcc attacctggc gcccaggcgt ggcagagccg ctcgaatccg
     3001 tcctggcccg atccttggcg gacggccgca tggctggcgg cgtgtcggtc atcgagtgga
     3061 cgccggtgac caggcgcttc accgcgcaga gcgagcgcat caggctcgcc ctgcgtgacg
     3121 cgctcgagtc agccagctcc gacgccgcga cgagcgcgta ctggtcagcc ggatggctac
     3181 gcaatgcggc ccgtttcgcc gccgccgccc ggagacaggt ccgtatacgc aacggcgacg
     3241 gccctatcgt catcgcctgc gccggcccga gccttatgcg gttccttccc gagcttagcc
     3301 gtcgccgcga cgatattgcc ttgtgggccc ttgcctcggc cgttccggcg ctcgtcgccg
     3361 ccggcctgac gcccgaccta gccgtagcca ccgatcccgg tttctggaac gccctgcacc
     3421 ttcggaccat cacggaactc ggaatcccga tcgcgctgtc cccgagcgcc ttcgccaccg
     3481 aggacgccct ctatcgatgc cctatcctag cgctcgatac tggcttaccg ttcgagcgtg
     3541 cggccgtcga ggctgcaggt ttcgaagacc atcgctgttc ggcgtccggt agcgccgccg
     3601 gcaccgcctt atcgctcgcg ctcgcatgta ccggaggtcc ggtaaccctc gccggctacg
     3661 acctggccgc cttcgggcat gaggaccatt gcaggcccta ccccttcgac tcgctcgacc
     3721 tagcgctggc ttcaagaacg aggcccgtaa cgtcggccgg cgcttccagg acgttcgacg
     3781 gctatcaggc ggccgtaggc gcttggcgcc tctctcgcgc attctccgcc tacgccttgt
     3841 cgattcggcc gccgctaggc gatagcgcca gggcgttcag gctctccgac tcccccgtgc
     3901 cgactccggt gcctcgcatt gccagactcg acgaccttcc gccgacgagg ggtgagccgc
     3961 ccgttgttac accgctcgac gatggctcgc gcgactcgag tctggactcg gcggccattc
     4021 ggcgtgaagg ctacctcgac cgcgtatcgt cgtcgctcga agcctcgctc gcaggcgcaa
     4081 ccgaggctct ggcctcgtcg gttccgatgc cgttcgatgc catgacggta tacagggccc
     4141 tcgctcccga ggccaccgcg cccctcgtcg cctcgatggc caggggcgag gcgacagaac
     4201 gcgaactcct cgccgtccac tcggcggccg agcaagccct cttttcgatg cgcggaggct
     4261 gcgatggctg aggctaacct ggctcacgag ccgctcgacg cgcagctctt cgagcgcaat
     4321 atgctggccg tggcctcgtg ccacgggcca gtctgcgctg acaggatacg ctcggcgcga
     4381 tctgatccgc gcgttggcgt ccaaacctcc cgttcggggc atccggtacc cttcgtcggc
     4441 ttaggctccc gccaagccct tatgcattcc aggttcgacc cggtgcgaga ggccgggcga
     4501 ctcgcctcga gctacagcaa ggcggcgttc tatgtcttcc tcgggctcgg ggcaggctat
     4561 gaggccgccg ccatgctcga ggccgacctc gtgaccaggg gtctgatcgt cgaatacggg
     4621 gccaccacgt tacgcgccgt gcttgaacgc gtcgacctaa cccatgtgct cggcgatcga
     4681 aggctcaggc tcctcgtcga tccggaggag gccgaactga acgaggttat cgccgggtgt
     4741 tacgtgccag cggtgaccgg cgacatcgtc tcggttcccc tgcgcggcag gatagacgcg
     4801 tcgcccgagc cgttccatag ggccgccgat gccatacgcc gatccataga cgccatatca
     4861 gacgactact cggttcaatc gttcttcggc aagcgctggt tctcgaatat cgtccgtaac
     4921 ctcgagctgt ccaacgcgcc gacgccgcct gtcgggccca tacgctcggc ctatattact
     4981 gcggccggcc cgtctctcga acgatacgcc gaggagatcc gccttaggcc caaggacgcg
     5041 tttctgatcg ccaccgacac gagcctgcgc gccttgctcg gtcggggcat tcggcccgac
     5101 gcggtgataa
//
LOCUS       DAOPHV010000134         5102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_21795_length_5102_cov_3.868437, whole genome shotgun sequence.
ACCESSION   DAOPHV010000134 DAOPHV010000000
VERSION     DAOPHV010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21795_length_5102_cov_3.868437"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLQ29_04880"
     CDS             <1..253
                     /locus_tag="PLQ29_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPG86013.1"
                     /translation="RAVDETGWKPAKVGQNGEGGRAFPLDQALPLKEAKRRMELAYIE
                     RQVELAGGSVSRAAERLGVLPNNLSRRIGELRETDSRAD"
     gene            complement(266..1159)
                     /locus_tag="PLQ29_04885"
     CDS             complement(266..1159)
                     /locus_tag="PLQ29_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86014.1"
                     /translation="MRASDTPLPKGGAAYLVMTVVARWSTLRAAASFAATVIGRFFLD
                     QYAHAARLRKGSSPVRVKNVEHPLDAIIPIRYEAARVYLTFVKLWMDALGYFRHRLGK
                     AFDGVVIEFLAGLGRCYSDAGSVYGRCLSTTRRPDTAPSLRLAFVYAVDPHLFCVPSL
                     HVLVVCYTYFRLRELLAATGEAERFGRELGALRSRAVEITESILYVRQHSVNCIPTAL
                     AMLSVIAPGYDEVEAKAFMAELFAREAEVEPGARARVLDYMTSLYDQVAPAGGSPEDR
                     YNAIIDFLKAYGEPVLELAEA"
     gene            complement(1156..2895)
                     /locus_tag="PLQ29_04890"
     CDS             complement(1156..2895)
                     /locus_tag="PLQ29_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPG86015.1"
                     /translation="MSDPEKNATIGSEAGGPGLDFIREAVAEDLRSGRFAYVRTRLPP
                     EPNGWMHIGHAKAFSIDYYVAKDFGGTCNLRFDDTNPAKEDMSYVEAIKRDTFWLGLD
                     CRDRVLFASDYYERMYELAVKLIKKGVAYVDDLSAEQMSEYRGTAVADKNNVTHTPPG
                     RDSPYRNRSVEENLDLFERMRAGEFADGAKTLRAKIDMAHPNLVMRDPPMYRIKREPH
                     YRTGNAWCIYPMYDFQEAMSDAIEGVTHSLCSLEYEIHRPLYDWFIREAEVFPSRQIE
                     FSRLNISHTVLSKRWLLRLVQDGVVSGWDDPRMPTIAGLRRRGYTPEAIRDFLGRIGI
                     AKTDSLVDIQLLEHCLREDLNRRADRVMAVLRPLKLIIENWPAGKVDELEAVNNPEDP
                     SAGARKVPFSGELWIERDDFAEVPPPKYFRLFPGNSVRLRYGYIVTCTGFDKDPATGE
                     VVAVRCTYDPETRGGNAPDNRKVKGTIHWVSAAHAVPMEARLFDHLFVAERPMDVPDG
                     VDWVTTVNPGSLEVLSGYAEPRLASAEPGRSFQFERLGYFCCDPDTRPGAPVFNRSVS
                     LRDTWAKIEKKQA"
     gene            complement(2963..4477)
                     /gene="gltX"
                     /locus_tag="PLQ29_04895"
     CDS             complement(2963..4477)
                     /gene="gltX"
                     /locus_tag="PLQ29_04895"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPG86016.1"
                     /translation="MKARVRYAPSPTGLQHIGSVRTALFNYLFARSVGGTFILRLEDT
                     DRTRSQDIYVKNLYDTFRWLGFYWDEGPDVGGPVGPYTQSARFALYREHAERLVDMGK
                     AYYCFCSDDKSAAKDAEKSGQDQAEQEGDAQASGGFGYPRTCRGLDPAEAKRRAASGE
                     PHVIRLKIPLDGVTGFDDILLGRIEWKNADVNPDPVLLKSDGFPTYHLANVVDDHLMG
                     ITHVMRAQEWIPSAPLHMIMYEAFGWEPPRLCHLPMVLGDDGHKLSKRHGATAVDEFR
                     RRGYLEAALINYVAMLGCSYEDGRDLYSLEDLARLFSMDHINKAPAVFDYVKLEWFNG
                     QYIRMLSDEELAARITPFMAEAGLVSAKPSALEVARLLAAAPLVKERLKYLSDAPEAL
                     RFLFAEPAVPPVEELLPKKMDAAGAARALGLAMELLEANDPADHDACEAAFRSGGAEA
                     GLKLGDLLMPLRVAVTGTKVSPPLFQSIAVLGKATAIQRAGKALAALKAANERP"
     gene            4590..>5102
                     /locus_tag="PLQ29_04900"
     CDS             4590..>5102
                     /locus_tag="PLQ29_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86017.1"
                     /translation="MKAIKPAIASVLLALAALSPVLAQDGEGSVFAPYPSRIRIGVRG
                     REIVLSWEDSSDIVSGYAVYRHDEFPDASNFERALLLGYSDSGSKGFAYAPVDDRPYY
                     YFVLGRVPEPVPEGSPAEYRIFIPLRNVPIEPVALAAQAVPAAAPTVAASSERPKAAK
                     PRLSGILARAD"
BASE COUNT          834 a         1628 c         1749 g          891 t
ORIGIN      
        1 gcgcgccgtc gacgaaacgg gctggaagcc ggccaaggtc ggccagaacg gagagggcgg
       61 gagagccttc ccgctcgacc aggccctgcc gctcaaggaa gccaagcgcc gcatggagct
      121 ggcctacata gagcgacaag tcgagctggc gggcggctcg gtaagtcggg ccgccgagcg
      181 gctcggcgtg ctaccgaaca atctttcgcg caggatcggg gagctccgag aaacggatag
      241 tcgggccgat taggccgtta ccgcctcaag cctctgccaa ctcgaggacg ggctcgccgt
      301 aggccttgag aaagtcgatt atggcgttat agcgatcctc gggactgcct ccagccggcg
      361 cgacctggtc gtacaggctc gtcatgtagt cgagcacgcg ggctctggca ccgggctcga
      421 cctcggcctc gcgcgcgaac agctcggcca tgaaggcttt ggcttcgact tcgtcgtagc
      481 ccggcgcgat gaccgacagc atcgctaagg cggtcgggat acagttgacg ctgtgctggc
      541 gcacgtagag tatcgactcg gtgatctcga cggctctcga gcgcagggcc cccagctcac
      601 ggccgaaacg ttcggcctcc cccgtcgcgg ccaacagctc gcgtagcctg aagtacgtgt
      661 agcagacgac gagtacgtgc aggctcggca cgcagaacag gtgcgggtcg accgcgtaga
      721 cgaaggccag cctgaggctc ggcgccgtat cgggccgtct cgtcgtcgac aggcagcgcc
      781 catagaccga gccggcgtcg gaatagcagc gtccgaggcc ggccaggaac tcgatgacga
      841 cgccatcgaa agccttcccg aggcggtggc ggaagtaacc gagcgcgtcc atccacagct
      901 tgacgaaggt cagatagacc ctggccgcct cgtagcgtat cggtatgatc gcgtcgagcg
      961 gatgctcgac gttcttgacc cgcacggggc tcgagccctt gcggagcctg gcggcgtggg
     1021 cgtactggtc gaggaagaag cgcccgatga cggtggcggc gaacgaggcg gccgcccgga
     1081 gcgtactcca ccgggcgacg accgtcatga cgaggtaggc cgcgcccccc ttcgggagcg
     1141 gggtgtccga ggccctcaag cctgcttctt ctctatcttg gcccaggtat cgcgcaacga
     1201 taccgagcgg ttgaataccg gggcgccggg cctcgtgtcc gggtcgcaac agaagtagcc
     1261 gagccgctcg aactggaacg agcgacccgg ttcggccgag gccaagcgag gctcggcgta
     1321 gcccgacagg acctcgaggg agccggggtt gaccgtcgtt acccaatcga cgccatcggg
     1381 cacgtccatc gggcgctccg ctacgaacag gtggtcgaaa agcctggcct ccatcggcac
     1441 ggcgtgggcc gcggataccc agtggatggt gcccttgacc ttgcggttgt ccggggcgtt
     1501 gccgcctcgg gtctccgggt cgtaggtgca gcggaccgcg acgacctcgc ccgtggccgg
     1561 gtccttatcg aagccggtgc aggtgacgat atagccgtag cgcaggcgta ccgaattgcc
     1621 ggggaagagc ctgaagtact tgggcggcgg aacctcggcg aagtcgtcgc gctcgatcca
     1681 tagctcgccg gagaacggta ccttcctggc gcctgcggac gggtcctcgg ggttgttgac
     1741 cgcctcgagc tcgtcgacct tgccagcggg ccaattctcg atgatcagct tgagcggcct
     1801 caagacggcc atgacgcgat cggcgcgccg gttcaggtcc tcgcgcaggc agtgctcgag
     1861 cagctggatg tccacgaggc tatcggtctt ggcgatgccg atgcgtccga ggaagtcgcg
     1921 tatagcctcc ggcgtgtagc ctcggcgcct gagaccggct atcgtcggca tgcgcggatc
     1981 gtcccagccg gagaccacgc cgtcctgcac gagcctgagg agccagcgct tactgagcac
     2041 cgtgtggctg atgttgaggc gcgagaactc gatctgccgc gaggggaaga cctcggcctc
     2101 gcggatgaac cagtcataca gcggtcggtg tatctcgtat tccagggaac agagcgagtg
     2161 tgtcacgccc tcgatggcgt ccgacatcgc ttcctggaag tcgtacatcg ggtagatgca
     2221 ccaggcgttg cccgtcctgt agtggggctc gcgcttgatg cggtacatcg gcgggtcgcg
     2281 catcaccagg ttcgggtggg ccatgtctat cttggccctg agcgtcttgg cgccgtcggc
     2341 gaactcgccg gcccgcatgc gctcgaagag gtcgagattc tcctcgacgg agcgattacg
     2401 gtacgggctg tcgcgaccgg gtggcgtgtg ggtgacgttg ttcttgtccg ccacggcggt
     2461 gccgcggtac tcgctcatct gctccgcgct caggtcgtcg acgtaggcga cgcctttctt
     2521 gatcagcttg acggccagct cgtacatccg ctcgtagtaa tccgacgcga acaggacgcg
     2581 gtcgcggcag tcgaggccga gccagaaggt atcgcgcttg atcgcctcga cataggacat
     2641 gtcttcttta gcaggattgg tgtcgtcgaa gcgcaggttg caggtgccgc cgaagtcctt
     2701 ggccacgtag tagtcgatgc tgaaggcctt ggcatggccg atgtgcatcc agccgttcgg
     2761 ctcgggcggc aggcgcgtcc gcacataggc gaagcgtcca ctcctcaggt cctcggcgac
     2821 ggcctcgcgt atgaagtcga ggccgggacc gccggcttcc gaccctatcg tcgcgttctt
     2881 ctcgggatcg ctcatgggta gctccttgta ccgcgcttca ggggcgcggc ggagtcagta
     2941 tgccccgcgc ccccgaaggc ggtcaaggac gctcgttggc agctttgagc gcggcgaggg
     3001 ccttcccggc gcgctggatg gccgtggcct tgcccagcac ggctatgctc tggaacagag
     3061 gagggctgac cttggtaccg gttaccgcca cgcggagcgg catcaggagg tcgccgagct
     3121 tgagtccggc ctccgcgccg cccgagcgga aggccgcctc gcaggcgtcg tggtcagccg
     3181 ggtcgttcgc ctcgagcagc tccatggcca gtccgagcgc ccgcgcggcg ccggcggcgt
     3241 ccatcttctt gggcagaagc tcctcgaccg gcggcacggc cggctcggcg aacaggaacc
     3301 gcagggcttc cggcgcgtcc gacaggtact tgaggcgctc cttgaccagc ggcgccgccg
     3361 ccagcaacct ggctacctct agcgcgctcg gcttggcgga tacgagtccg gcctcggcca
     3421 tgaagggagt aatcctcgcg gccagctcct cgtcggacag catgcggatg tactggccgt
     3481 tgaaccactc gagcttgacg tagtcgaaga cggccggcgc cttgttgata tggtccatgc
     3541 tgaacaaccg ggccaggtcc tcgagcgagt agaggtcgcg gccgtcctcg taggagcaac
     3601 cgagcatggc cacatagttt atcagcgccg cctcgaggta accgcgcctg cggaactcgt
     3661 cgacggccgt ggcgccgtgg cgtttactga gcttatgtcc gtcgtcgccg agcaccatcg
     3721 gcaggtggca gagccggggc ggctcccagc cgaaggcctc gtacatgatc atgtggagag
     3781 gagccgacgg tatccactcc tgggcgcgca tcacatgggt gatgcccatc aggtggtcgt
     3841 cgacgacgtt ggccaggtgg taggtcggga agccgtcgct cttcaggagc accgggtcgg
     3901 ggttgacgtc ggcgttcttc cactcgatac ggccgagcag gatgtcgtca aagccggtca
     3961 cgccgtcgag cggtatcttg agccgtatca catgtggctc gcccgaggcg gcgcgacgct
     4021 tggcctcggc cggatcgagg ccgcggcagg tgcggggata gccgaagcca cccgaggcct
     4081 gggcgtcgcc ctcctgctcg gcttggtctt ggccggactt ctcggcgtcc ttggcggccg
     4141 acttgtcatc ggagcagaag cagtagtagg ccttgcccat gtcgacgagg cgctcggcgt
     4201 gctcgcggta cagcgcgaag cgcgccgact gcgtatacgg gccgacgggg ccgccgacgt
     4261 ccggaccctc gtcccagtag aagccgagcc agcggaaggt gtcgtacagg ttcttgacgt
     4321 atatgtcctg cgaccgggtg cgatcggtgt cctcgagcct gaggatgaag gttccgccga
     4381 ccgacctggc gaacaggtaa ttgaacaggg cggtgcgaac gctccctata tgctgcaggc
     4441 ccgtcggcga cggggcgtat ctgactctgg ccttcatcgt ggctatcctc cgttgctgag
     4501 tactcagacc gcgagtatat cggcctccgc ggggcctgat caatcctgcg ctatggagcg
     4561 agcttcaatt ttccgatact ataggggata tgaaagcgat aaaacccgcg atcgcgtcgg
     4621 tgctgctagc gctggcggct ctgtcccccg tcctggccca ggacggcgaa ggctcggtgt
     4681 tcgcgcccta cccgtcacgc atacggatag gcgtgcgcgg ccgcgagatc gtgctgtcct
     4741 gggaggacag ctccgatata gtgtcgggct acgcggtcta ccgccacgac gagttccccg
     4801 acgcgtcgaa tttcgagcgg gccctgctcc tcggctattc cgatagcggc tccaagggtt
     4861 tcgcctacgc gccggtcgac gataggccat actactattt cgtgctcggg cgcgtccccg
     4921 agccggtccc cgaaggctcg ccggccgagt accgtatctt catcccgcta cgcaacgtac
     4981 cgatcgagcc ggtggccctg gcggcccagg ccgtcccggc ggccgcaccg acagtcgcgg
     5041 cgagctccga gaggcccaag gccgcgaagc ctcgcctctc cggcatcctg gccagggccg
     5101 at
//
LOCUS       DAOPHV010000135         5082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_21904_length_5082_cov_4.532922, whole genome shotgun sequence.
ACCESSION   DAOPHV010000135 DAOPHV010000000
VERSION     DAOPHV010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21904_length_5082_cov_4.532922"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2744)
                     /locus_tag="PLQ29_04905"
     CDS             complement(<1..2744)
                     /locus_tag="PLQ29_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86018.1"
                     /translation="MRLKSSFSVLSTTVAMYVMAFAMAPRVARADEPPVIGFDTVSYE
                     HAVAMADYEQGSGRWMLVARTKADRELARWELLASESYPDDRDVARARADLADWSDEY
                     IAERYAAWLEDRFFGEESGRLRGLVSEAASRANRAFLYVCDDDGMVSFDDAGDPIMLA
                     RGADGTIASDKEAWLEMTESSMREALARFDLDAEASLNAYLPELSAYIGPDLSRYESI
                     AASAATSARDRVVSELDALIAREERLFVARREADSWSLRKRSDGEAADSIVESLIAEV
                     HGACGAALESLRERLDGPSGTTSELDLSGSDWLERFEEQFDAGLELWTEAEERFMVRR
                     LEWERDAATAFADGGAAWSKAYNRLVAERSAWEAKAGELLLSGERAFGGASERLESSI
                     AEARAEFERDAAERGLASSARASAWADVYVQSGQAAMGAKSGAEYWLSSLGAVGLLVG
                     SAELTAWLDGAEAGASASRLASIAKARECIERYDEFMARCSEARGRLIDDFGLAIGSG
                     AGSLVDVLAEGADSLSFCLDEYQVELLRAEAVEGYWRSRVSIAEAVSAYAEDLSSGRA
                     TAGDTAAGLAAARSAYDAAASAYDQAKSDLSEAGSRIGEASEGIGFAFDALQESGLRL
                     ERAASEYSSLMVELASGGNGEWLRSELLTRYRDLVESAGLSADTSAASEAELLAAYYE
                     RAVECGQFKAIEAAGRAMRSAVEGVGEEPSLRTLANAAAAVVEPSSLYDWSSEGVGSG
                     ELFAASYGLSTADPAYASLLALHDHTASALTGASSDDRRAELIASSLELAGRLARRAD
                     SLAESALEARLDAVALLGAVDSAAWYGSRTGGSLGDLSLEDRLRSDEAGALIAWLEAR
                     ARLELDAILVLEGSMRPDLASDEAMALCRAFLSLGGSASLVGRHGTLEAIIGLIDLNR
                     S"
     gene            complement(2746..>5082)
                     /locus_tag="PLQ29_04910"
     CDS             complement(2746..>5082)
                     /locus_tag="PLQ29_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPG86019.1"
                     /translation="VIPGGTGEAIRLGSIARVDSRQREPESLSRVDGRRAVTIAVIPT
                     GDANLAMLSRDIACEAAKAASACGIRFTVLSDRGELAARSLKRVISAAIEAVVVVSVA
                     AALVIGPRLYLGRSRVRSLAMVASAAMVPFILVVSAAILSCLMRGLSVGALAGLAIGL
                     GSSIDCSILVAERLGSSKTVGSGAASVRELAPALAAGTATTILALAPMFGLEAVSPGV
                     GAVAAGIAVTCGVSYVATIAIMPPVVLWGGDMVGLRRRSVEMARRLHRLRVGRPTTPR
                     ARRQSARLLAAAIAVCAERPRLVLAWSAAIGLAGVVAISISPKDVGTRTEGRDLYARL
                     EFDQGLPLEAVDSRLADYAASLARLPGLVSTQSSARRGSANLLLTYDDDVADSASLAA
                     SAKLAAPAGSFLWIESRAPDERVWELLVRGDDDETCRSMAAAAAERIAALPLALEVAL
                     NFKVSAPAIVVKPDRDRSSAMGLPFSIVAGAMRRSVQAPVAYKRVGDTGDTDVRIGAS
                     RRVDEEALGSIVVASRGGATLEAASLVRIGREERIARIQRRDRRRSASLSIRTRRMDP
                     EKAARETMAALEGLVTPPGYTLTFDRDAIEAARRLRRVTLSFVAAVALAYMAMAALSE
                     SFGLPLVALAAVPPSLAAPALILVATGSPLDASMACAFVAISGVAVNASVLMADYIRK
                     AKKVGFVASAMALYRVVRSRIVIVSATAATTVVGAIPLLVLDTGDMGMVRALAFVTAC
                     GGAASFMAAVTVVPALAAAAPRLFDRFGPSAPPRDGGD"
BASE COUNT          902 a         1800 c         1583 g          797 t
ORIGIN      
        1 gagcgattca ggtctatcag gccgatgata gcctcgagcg taccatgtcg tccgacgagc
       61 gaagccgacc cgccgaggct caggaacgcg cggcatagcg ccatcgcttc gtcactcgcc
      121 agatccggtc gcatggagcc ctcgaggacc aggatcgcat cgagctccaa gcgggccctg
      181 gcctcgagcc aggcgatgag cgcgccagct tcatcagacc tgagcctgtc ctcgaggctc
      241 aggtcgccga gcgaaccgcc ggtcctcgag ccgtaccagg ccgcggagtc gacagcgcca
      301 agcaaggcta ctgcgtcaag gcgagcctcg agcgccgact cggccaggct atcggctcgt
      361 ctggccaggc gaccggcgag ttctaggctt gaggcgatca gctcggctct tctgtcgtcg
      421 ctagacgcgc ctgtgagcgc cgacgcagta tggtcgtgca gggccaggag cgaggcgtag
      481 gctggatccg cggtcgacag cccgtagcta gcggcgaaga gctcccccga accgacgccc
      541 tcggagctcc aatcgtatag ggaagacggt tcgactacgg ccgcggcggc gttcgccaac
      601 gtacgtaggc tgggctcttc gccgacgcct tcgacagctg aacgcatggc ccttcccgcg
      661 gcctcgatcg ctttgaactg accgcattcc acggccctct cgtagtacgc cgcgagcagc
      721 tccgcttcgc tggcggccga cgtgtcggct gataagccag ccgattcgac gaggtcgcgg
      781 tatcgggtca acagctcgga tctcagccac tcgccgttcc cgccggaggc caattcgacc
      841 atcagcgatg agtactcgga cgccgcacgt tcgagcctca gcccggattc ttgaagcgcg
      901 tcgaaggcga agccgatgcc ctcggaggct tcgccgatac ggctacccgc ttcggataaa
      961 tccgacttgg cctggtcata ggccgaggcc gccgcgtcgt aggctgaccg cgccgccgcc
     1021 agtcctgcgg ccgtatcccc ggcggtagcc ctgcccgagc tcagatcctc cgcgtaggcg
     1081 ctcacggctt ccgctattga caccctcgag cgccagtatc cttcgacggc ctcggccctg
     1141 agcaactcca cttggtactc gtccaggcag aacgacagac tgtctgcccc ttcggctagc
     1201 acgtcgacca aggagccggc tcccgagcct atggccagcc cgaagtcgtc gatcagccga
     1261 cctcgagcct cggagcatct ggccatgaac tcgtcataac gttcgatgca ttcgcgcgcc
     1321 ttcgctatcg aggccagcct agacgccgat gccccagcct cggcgccgtc tagccaagcc
     1381 gtcagctcag ccgagccgac cagtagccca acggcgccga gcgacgatag ccagtattct
     1441 gcgccagact tggcgcccat ggccgcctgc ccgctctgga cgtacacgtc ggcccaggcg
     1501 ctggccctcg cggagcttgc cagcccccgt tccgcggcgt ctcgctcgaa ctcggccctc
     1561 gcctcggcga tggacgactc gagtcgctcg ctagcgccgc caaacgcccg ttcgccgctc
     1621 aggagtagct cgccggcctt agcttcccat gccgaccgtt ccgccacgag acgattgtac
     1681 gccttggacc atgccgcgcc accgtccgcg aacgccgtag cggcgtctcg ctcccattcc
     1741 agcctgcgca ccatgaaacg ctcctcggct tcggtccaaa gctcgaggcc ggcgtcgaac
     1801 tgctcctcga atcgctccag ccaatccgat ccggataggt ccagctcgct ggtcgtgcct
     1861 gaggggccat ccaggcgctc gcgtagcgac tccaaggccg cgccgcaggc cccgtgaacc
     1921 tcggctatca gcgactcgac aatcgaatcg gcggcctcgc cgtcactccg cttcctgagg
     1981 ctccacgagt ccgcttccct tcttgcgacg aacaggcgct cctcgcgggc gatcaaggcg
     2041 tcgagctcgc taactaccct gtcgcgggcg ctggttgcgg cggaggcggc tatcgactca
     2101 tatcgcgaaa ggtctgggcc gatatacgcc gagagttctg gcaagtacgc gtttaggctt
     2161 gcctcagcgt ccaagtcgaa ccgcgctaga gcctcgcgca tggaggactc ggtcatctcg
     2221 agccaagcct ccttgtccga ggctatagtc ccgtcggcgc cgcgagccag cataataggg
     2281 tcgccggcat cgtcgaacga caccattccg tcgtcatcgc atacatatag gaacgcgcga
     2341 ttcgccctgg aggccgcctc cgacacgagc ccacgtaggc gccccgattc ctcgccgaag
     2401 aatctatcct cgagccatgc ggcgtaacgc tcggcgatat actcgtcgct ccaatcggcg
     2461 aggtcggctc gggcgcgggc cacgtcgcga tcgtccgggt acgactcgga cgcgagtagc
     2521 tcccaacgtg cgagctccct gtcggccttc gtcctggcga cgagcatcca tcgcccggag
     2581 ccctgttcgt aatcggccat ggcgaccgcg tgttcatacg ataccgtgtc gaagccgatc
     2641 accggcggct cgtcggcgcg agcgacgcga ggcgccatgg cgaaggccat gacgtacatg
     2701 gcaaccgtgg tcgacaagac gctgaacgac gacttgagcc gcatactagt cacctccatc
     2761 gcgtggtggg gccgaaggcc cgaatctgtc gaacaggcgc ggcgctgccg ccgccagggc
     2821 cggtacgacg gtaacggcgg ccatgaagga agccgctcca ccgcaggcgg tcacgaaggc
     2881 taacgccctg accatcccca tgtcgcctgt atcgagaacg aggagaggta tcgctccaac
     2941 gacggtcgta gccgcggtcg ccgatacgat gactatcctc gaacgcacta cgcggtagag
     3001 agccatggcg cttgcgacga aaccgacttt tttggccttt cgtatatagt cagccatgag
     3061 caccgaggcg ttgacagcta cgccggatat tgccacgaag gcgcaggcca tcgatgcgtc
     3121 gagcggcgag ccggttgcca ccagtatcag ggccggcgcg gccagcgaag gaggcaccgc
     3181 ggcgagcgcg acgaggggca gtccgaacga ctcgctcaac gccgccatgg ccatgtaggc
     3241 cagcgccacc gccgctacaa aggacagcgt gacccgcctc agccgcctgg cagcttcgat
     3301 cgcgtctcgg tcgaaggtca gcgtatagcc gggaggcgta acgagcccct cgagcgcggc
     3361 catcgtctcc cgcgcggcct tctccggatc catgcgtctc gtccggatcg acagggatgc
     3421 cgacctgcga cggtctcgtc tctgtatcct ggctatccgc tcctcgcggc cgattcggac
     3481 cagggacgcg gcctccaggg tcgcgccgcc ccgcgaggcg accacgatcg agccgagcgc
     3541 ctcctcgtcg actcgacggg aagcgcctat gcgaacatcg gtgtcgcccg tatcgccgac
     3601 ccgtttatac gcgaccggcg cctggaccga tcgccgcatg gctcctgcca ctatcgagaa
     3661 cggcaacccc atggcggacg acctatcgcg gtcgggcttg acgacgatag ccggcgccga
     3721 gaccttgaaa ttcagggcga cctcgagagc caggggtagc gccgcgatac gctctgcggc
     3781 ggccgccgcc atcgagcgac aggtctcgtc atcgtcgccg cgaacgagca gttcccagac
     3841 ccgttcgtcg ggcgcccggc tctctatcca caggaagctc cctgccggag cggccagctt
     3901 cgcagacgcc gcgaggctgg cactatcggc gacgtcgtca tcgtacgtca acaataggtt
     3961 ggctgagccg cgcctggcgc tcgactgagt cgagaccagg ccgggtagcc tagccaacga
     4021 cgccgcatag tcggcaagcc tcgagtcgac cgcctcgagt ggcaggccct ggtcgaactc
     4081 gaggcgcgcg tataggtcgc gcccctccgt tcgggttccg acgtctttcg gagagatcga
     4141 gatggcgacg acgccggcca gcccaatcgc ggccgaccag gcgagtacga gccgcggcct
     4201 ctcggcgcat accgcgatag cggccgccag aagccgagcc gactgccttc tcgctcgcgg
     4261 agtggttggg cggccaaccc tgagacggtg aagacgccgt gccatttcta ccgatcgtcg
     4321 cctcaacccg accatatcgc caccccacag gacgacgggc ggcatgatcg ctatcgtcgc
     4381 cacgtacgag acgccgcagg tgacggctat gcccgccgct accgcgccga cgcccggcga
     4441 gacggcctcg agtccgaaca tcggagccag cgccaagata gtggtggcag tacccgcagc
     4501 cagggccggc gccagctccc ggaccgaggc cgcgcccgag cccacggtct tggatgagcc
     4561 gagacgttcg gccacgagta tcgagcaatc gatcgaagaa ccgagcccga tggccaagcc
     4621 ggctagagcg ccgacgctca gtccgcgcat caggcaggac aggatcgccg cggagacaac
     4681 caatatgaac gggaccatag cggccgaggc gaccatagcg aggctacgca cgcgcgaacg
     4741 cccgaggtag agcctgggtc cgattacgag cgcggccgcg accgatacga cgacgaccgc
     4801 ctctatcgcc gccgagataa cgcgcttcag cgatctggcg gccagctccc ccctgtcgga
     4861 cagtacggta aaacggatcc cgcaggcgga agcggccttc gccgcctcgc acgcgatatc
     4921 tctcgacagc atggcgaggt tggcatcgcc ggtcggtatg acggcgatgg tcacggccct
     4981 gcgcccgtcg acgcgggaca ggctctcagg ctccctttgg cggctatcca ccctggctat
     5041 cgagccgagg cgtatagcct cccccgtgcc gcccgggata ac
//
LOCUS       DAOPHV010000136         5050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_22114_length_5050_cov_4.827828, whole genome shotgun sequence.
ACCESSION   DAOPHV010000136 DAOPHV010000000
VERSION     DAOPHV010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22114_length_5050_cov_4.827828"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2134
                     /locus_tag="PLQ29_04915"
     CDS             <1..2134
                     /locus_tag="PLQ29_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HPG86020.1"
                     /translation="LVIDAAEALASPGCVRVITAADVPGSARFGQIEKDYPIFCSGRV
                     RSYGDVCALVVAETRAAAKAAAALVRVTATPVRPVLTIDEALEPGAPVVPSYGETNVV
                     THHRLRHGDPEPVFAGCELVLEEEFSTQAIEHAYMEPETAVCVPRSDGVVEVYGSMQH
                     PFSTRRFVAALLGESLSNVEVYTIAVGGGFGGKDDTAAAVCARAALAARLTGRPVKLR
                     YDREWSFRESYKRHPYRLRYKVGVSSEGRVLAVKATIHADSGAYLSVTPWVTWRSTAQ
                     CFGPYVVDAIHADVYGVATNNVFTGAMRGFGAPQVNFAVEQLMDMAAHELKIHPLGMR
                     RINMVKQDSVTVTGQKLDGHVVSMEDVMNRVVHEIGYRKKYEHCSFGTPSEEGSDELY
                     GIGLAISYRGASLGAEGMDFCSCIINGQYDGSILLETGIHENGQGAESAMTLILAEEL
                     GVDLSRVRYKRSSTSSIPDSGTTVATRGTIMGGSAVVVAARQYRELLSRHLADRLRCR
                     PEDVTFHDDKIWGLNYEYSLSWEEAMRELFLQRVTPYAFGTFQAPPVSWDDTTGQGDA
                     YFTYVYSCQAVELTVDRKTGKIRLRSIVAGHDIGKAVNRALLLGQVYGGVTQGVGMAL
                     MEDFAYEGGRPVCENFDSYLLPRATDLPEMTAVIVENHDPASLTGAKGIGEPALELIA
                     PAIANAVYNATGVRCRDLPIRLRPEDLA"
     gene            2131..2637
                     /locus_tag="PLQ29_04920"
     CDS             2131..2637
                     /locus_tag="PLQ29_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPG86021.1"
                     /translation="MNQITINGVAHTVGDEERALSLLEYLRERRFLTGTKNGCEKGLC
                     GSCTVVVDGKALRSCRIPVEKALGASVVTIEGMEGPGGEPHPIQQAFMDAGAIQCGFC
                     TPGMVMSAYALLLRDPDPSRDDIRKALKGNLCRCTGYQQIVDAVRLAAKVMREQALPV
                     EAGCLPRR"
     gene            complement(2623..3696)
                     /locus_tag="PLQ29_04925"
     CDS             complement(2623..3696)
                     /locus_tag="PLQ29_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPG86022.1"
                     /translation="MSRTTNRPARSAFASTLCAALCMALCAVAAASCSAAPRDRADLV
                     IGTVCSVRILDGGDRRALDAAFERLRGIEASMSANAEGTVVAAINAGAGAAPVPAPAD
                     VRYVIRKALSYAELSGGAFDPTIGPVVKLWNIGMDGERIPAPEEIAEALPLVDASAVA
                     VDDAAGTVFLPRAGMMLDLGAIAKGYAADEVARVLLEHRVKAAVVDLGGNVKVVGRKP
                     DGSPWRIGIQDPFDDRGSRIGIAELEGGATVVTSGVYERYFIGDDGAHYHHILDPATG
                     YPARNGLVSVTVISSSSIDADGLSTTLFSLGLERGMALVETLDHVEAVFIDEDRRVYQ
                     SSGAGKLFKLDNLAFTLTALPSR"
     gene            complement(3713..4366)
                     /locus_tag="PLQ29_04930"
     CDS             complement(3713..4366)
                     /locus_tag="PLQ29_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPG86023.1"
                     /translation="MSVGPIGENVYIVENPGSPVLVVDPGAEPERLAAEVLTAVRRAG
                     TDRVIVALTHGHLDHVAALPGLLAALAAAGIPVRTYAPEADRDYFGDKARETNERVFS
                     GIGAMGFLRKQWAPIPEADEYFGDGFAFPGLDAVAVHTPGHTRGSSCFLVEGGSSLLA
                     GDTLFRDGRGRTDNFDSDEAAIVHSIRERLCPLHDAVRVWPGHGEPTTIGREKRYYS"
     gene            4432..>5050
                     /locus_tag="PLQ29_04935"
     CDS             4432..>5050
                     /locus_tag="PLQ29_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86024.1"
                     /translation="MFVPVTLAILAAILFYALVPIAGAFVVRQQWRLFRKSVAEASTL
                     PELDSRSAARSTLGRVRALGEVDAISGLDELWIRCEGAACAVDLKDAWVYLLVGRSGD
                     DTVERRRWSDLRAITPGSRAFVAGEAEMRDGRVVMAQRDKSPPLVILHDGDDDRVVRR
                     AIWAGRHENEYWNPVTQVSLAFGAAAMSAIVPLALPGRMPSLISAL"
BASE COUNT          830 a         1763 c         1695 g          762 t
ORIGIN      
        1 cctcgtcatc gacgcggcgg aggcgctggc gagcccgggc tgcgtgcgcg tgatcaccgc
       61 ggccgacgtg cccggctccg cgcgcttcgg ccagatcgag aaggactacc ccatcttctg
      121 ctccggccgc gtccgatcct acggcgacgt atgcgccctg gtcgtcgcgg agacgagggc
      181 cgccgccaag gccgccgccg ccctcgtacg ggtcacggcc acgccggtca ggccggtgct
      241 gaccatcgac gaggccctcg agcccggagc gccggtcgtg ccgtcctacg gcgagacgaa
      301 tgtcgttacc caccaccgcc tgcgccatgg cgaccccgag ccggtcttcg ccggctgcga
      361 gctcgtgctg gaggaggaat tcagcaccca ggcgatcgag cacgcgtaca tggagccgga
      421 gacggccgtc tgcgtgccgc gatccgacgg cgtggtcgag gtctacggca gcatgcagca
      481 cccgttcagc accaggcgct tcgtcgccgc gctgctcggc gagagcctat ccaacgtcga
      541 ggtgtacacg atagcggtcg gaggcggctt cggcggcaag gacgataccg cggcggccgt
      601 ctgcgcccgc gcggccctcg ccgcccgcct gaccggccgt ccggtcaaat tgcgctacga
      661 ccgcgagtgg tcgttccgcg agagctacaa gcgccatccc taccggctgc gctacaaggt
      721 cggcgtctcg agcgagggcc gcgtcctggc ggtcaaggcc acgatccacg ccgactcagg
      781 cgcctacctg tcggtgacgc cctgggtcac ctggcgctcg acggcccagt gcttcggccc
      841 ctacgtcgtc gacgccatcc acgccgacgt ctacggcgtc gcgacgaaca acgtattcac
      901 cggcgccatg cgcggcttcg gcgccccgca ggtcaacttc gccgtcgagc agctcatgga
      961 catggcggcc cacgagctca agatccaccc gctcggcatg cgccgcatca acatggtcaa
     1021 acaggactcg gtcaccgtca ccgggcagaa gctggacggc cacgtcgtca gcatggagga
     1081 cgtgatgaac cgcgtcgtcc acgagatagg ctaccgcaag aagtacgagc attgctcgtt
     1141 cgggacgccg tcggaggagg gctccgacga gctgtacggc ataggcctgg ccatcagcta
     1201 ccggggcgca tccctcggcg ccgagggcat ggacttctgt tcctgcatca tcaatggcca
     1261 gtacgacggc tccatcctgc tggaaaccgg catccacgag aacgggcagg gcgccgagtc
     1321 ggcgatgacg ctgatcctcg ccgaagagct cggcgtcgat ctcagccgcg tccgttacaa
     1381 gcgctcgtcg accagctcca tccccgactc gggcaccacc gtcgccacgc gcggcaccat
     1441 catgggcggc tccgccgtcg tggtcgcggc caggcagtac cgcgagctcc tgtcgcggca
     1501 cctggccgac cgcctgcgct gcaggcccga ggacgtcacc ttccacgacg acaagatctg
     1561 gggcctgaac tacgagtaca gcctgagctg ggaggaggcg atgcgcgagc tgttcctgca
     1621 gcgcgtcacc ccctacgcct tcggcacctt ccaggctccg ccggtgagct gggacgacac
     1681 gaccggtcag ggcgacgcgt acttcaccta cgtctattcg tgccaggcgg tcgagctgac
     1741 ggtcgatcgg aagaccggca agatcagact acggagcatc gtggccggcc acgacatcgg
     1801 caaggcggtc aaccgggccc tcttgctcgg ccaggtatac ggcggcgtga cccagggcgt
     1861 cggcatggcg ctcatggagg acttcgccta cgagggcggc aggcccgtct gcgagaattt
     1921 cgactcgtac ctgctgccga gggcgactga tctgccggag atgaccgccg tgatcgtcga
     1981 gaaccatgac ccagcctccc tgactggagc caagggcatc ggggagccgg cgttggaatt
     2041 gatagcgccg gccatcgcca acgccgtgta caacgccacc ggcgtgcgtt gccgcgacct
     2101 gccgattcga ctcagaccgg aggacctcgc gtgaaccaga taacgatcaa cggcgtcgcg
     2161 catacggtgg gcgacgagga gcgcgcgctg tcgctcctcg agtacctgcg cgagcgccgc
     2221 ttcctgacgg gcaccaagaa cggctgcgag aagggtctct gcggctcctg caccgtcgtc
     2281 gtcgacggta aggccctccg atcctgccgg attcccgtgg agaaggcgct cggcgcgtcg
     2341 gtggtgacca tagagggcat ggagggcccc ggcggagagc cgcacccgat ccagcaggcc
     2401 ttcatggacg ccggagccat ccagtgcggc ttctgcacgc ccggcatggt gatgagcgcc
     2461 tacgctctcc tgctacgcga ccccgacccg agccgcgacg atatccgcaa ggccctcaaa
     2521 ggcaacctct gccgctgtac cggctatcag cagatcgtcg acgcggtccg gctagccgcc
     2581 aaggtcatgc gcgagcaggc gctaccggtc gaggctggct gcctaccgag acggtagcgc
     2641 tgtcagggtg aacgcaagat tatcgagctt gaacagcttc ccggcgccgg agctctggta
     2701 gacgcgccgg tcctcgtcga tgaagacggc ttcgacgtgg tcgagcgtct cgacgagggc
     2761 catgccccgc tccaggccca gggagaacag cgtcgtcgac aggccgtcgg cgtcgatcga
     2821 cgacgagctg atcaccgtga ccgagacgag gccattcctc gccggatagc cggtggccgg
     2881 atccaatata tggtggtagt gcgctccatc gtccccgatg aaatagcgct cgtagacgcc
     2941 gctcgtcacg accgtcgcgc cgccctcgag ctcggctatg ccgatgcgcg agcctcggtc
     3001 gtcgaaggga tcctggatgc caatgcgcca gggagagccg tccggcttcc ggccgaccac
     3061 cttgacgttg ccgccgaggt cgacgacggc ggccttgacc cgatgctcga gcaggacgcg
     3121 ggccacctcg tcggcggcgt agcccttggc gatggcgccg aggtcgagca tcatgccggc
     3181 ccgaggcagg aagacggtgc cggccgcgtc gtccacggcg acggcgctcg cgtcgacgag
     3241 gggcagggcc tcggctatct cctccggagc ggggatccgc tcgccgtcca tgccgatgtt
     3301 ccacagcttg acgacggggc cgatggtcgg gtcgaaagcg ccgcccgaca gctccgcgta
     3361 cgatagcgcc tttcgtatga cgtagcggac gtcggccggg gcgggtaccg gagcggcccc
     3421 ggcgccggcg ttgatcgccg cgaccacggt accctcggcg ttggcgctca tcgaggcctc
     3481 tatgccgcgc agccgctcga aggccgcgtc gagcgccctc cggtcgccgc cgtcgagaat
     3541 gcgcaccgag catacggtgc cgatcaccag gtcggcccgg tcccgcgggg cggccgaaca
     3601 cgatgcggcg gctaccgcgc ataacgccat gcataacgcc gcgcacaacg tcgacgcgaa
     3661 cgctgaccga gccggtcggt ttgtcgttct gctcatcggg tatcctccgg gatcaggaat
     3721 agtagcgctt ctcgcggcct atggtcgtcg gttcgccatg gcccggccag acgcgtacgg
     3781 cgtcgtgcag ggggcacagc ctttccctga tggaatggac gatcgccgcc tcatccgagt
     3841 cgaagttgtc ggtacggccg cgaccgtcgc ggaacagcgt atcgccggcc agcagcgacg
     3901 agccgccctc gaccaggaag cacgacgaac cgcgcgtatg ccccggcgta tgcacggcga
     3961 ccgcgtccag ccccgggaac gcgaagccgt cgccgaagta ctcgtcggct tccggtatgg
     4021 gcgcccactg cttgcgcagg aagcccatcg ccccgatgcc ggagaacacg cgctcgttcg
     4081 tctccctggc cttgtcgcca aagtagtcgc gatccgcctc gggcgcgtag gtgcggacgg
     4141 gaattccggc cgccgccagg gcggccagca gaccgggcaa agccgcgacg tggtccaggt
     4201 ggccgtgggt cagggcgacg atgacccggt ccgtcccggc ccgccggacg gccgtcaaga
     4261 cctcggccgc caggcgctca ggctcggcac cgggatcgac gaccagtacg ggagagcctg
     4321 gattctcgac gatatagacg ttttccccta tggggcctac actcatgctg cggatcatgc
     4381 ccggcgatac tacccggccg ggcgcctcga tgacaagcgg agcgctaccc gatgttcgta
     4441 ccggtgaccc tggccatact ggccgccata ctcttctacg cgctcgtgcc catagccggc
     4501 gccttcgtcg tacgccagca gtggcgcctc tttaggaaat cggtcgccga ggcctcgacg
     4561 ctgcccgaac tggactcgcg atcggccgcc cgatcgacgc tcggccgcgt ccgcgcgctg
     4621 ggcgaggtcg acgccatcag cggcctggac gaactctgga tacgctgcga gggcgccgcc
     4681 tgcgcggtcg acctgaagga cgcctgggtc tacctactcg tcggcagatc gggggacgac
     4741 actgtcgagc gccgacgatg gagcgacctt cgggccatca cgccgggttc cagggccttc
     4801 gtcgccggcg aagccgagat gcgggacggc cgcgtcgtca tggcccaacg ggacaagtcg
     4861 ccgccgctgg tcatcctgca cgatggggac gacgaccggg tcgtcaggcg ggccatctgg
     4921 gcgggccgtc acgagaacga gtactggaac ccggtcaccc aggtatcgct agccttcggg
     4981 gcggcggcga tgagcgccat agtaccgctc gcgttacccg gcaggatgcc ttcgctgatc
     5041 agcgcgctca
//
LOCUS       DAOPHV010000137         5047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_22131_length_5047_cov_4.445513, whole genome shotgun sequence.
ACCESSION   DAOPHV010000137 DAOPHV010000000
VERSION     DAOPHV010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22131_length_5047_cov_4.445513"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1990)
                     /locus_tag="PLQ29_04940"
     CDS             complement(71..1990)
                     /locus_tag="PLQ29_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPG86025.1"
                     /translation="MSPAVKSLVSRSMPGYAGAAARAAAIARAGGLDEALASGALQRD
                     QDVPLAEALVLGLLRQGVHKYVGVFGHGSTAVGEALRIYEAAGLVRAFNVRSEIEASH
                     VAAALRWQYGETAAVFTSIGPGALQALAASIAPASNGLGLYYLFGDETSQDEGYNMQQ
                     IPRQEQVCFLKAASAMGAAYSLLDPWALPSALKRGATTVFAADRAAPFYLFLPMNAQA
                     AVLPSFNLEELPRRPSFQRLAPADDAPFVEAARMIAAAERVTLKAGNGARGVDPRLLD
                     ELLRRADAAYVHGPSAVGVLPGSHPRNMGVGGSKGSISGNAAMARAELAIVVGARAVC
                     QWDSSGTAWASARSVIAINARPEDAAHYNRSLPLVGDAAVLVERLVQALRDAGVDKGS
                     SPTRWYGECRERRAEWDALIAGRLAREKLYDSKLGREAMTQPAAIAVVAAFAREKGAV
                     KYFDAGDVQANGFQLVEDETLGATFTETGASYMGFAASGILASALADSPSYPIAFTGD
                     GSFMMGNQVIIDAVEYGLRGMVVVFDNRRMAAISSLQRAQYCVDYRTDDRAVVDYAAM
                     ASSVEGVLGVHGGYSVLELRAALEAAYAHTGLSVIHVPVYAGSDDEGGLGAYGDWNVG
                     AWCERVQCEKHRLGW"
     gene            complement(1987..2619)
                     /locus_tag="PLQ29_04945"
     CDS             complement(1987..2619)
                     /locus_tag="PLQ29_04945"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF006753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HPG86026.1"
                     /translation="MRLAILSETSAADRNADILRALEPHGYELLNCGMRRSGEAPELS
                     YLHTGLMAALLLGLGRVDFVIGGCGTGQGFLNAALLYPGVSCGHVLCPLDAWLFARIN
                     GGNCVSLALNQGYGWAGDVNLRLVFEQLFTPERGAGYPEHRSMPQRASRELLGRLSNL
                     THRPMAELVSALPDEVALPALRYPGFLEAALTGRPKDEALAAAMAARAEA"
     gene            complement(2616..3584)
                     /locus_tag="PLQ29_04950"
     CDS             complement(2616..3584)
                     /locus_tag="PLQ29_04950"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPG86027.1"
                     /translation="MSAGVAQRAAFGRALVELAERYPKMVVLDPDVCPSTQTSLFRAA
                     YPDRFYPMGIAEANAVCVAAGLASCGFVPWVSAFAVFLATRAADQVRVSVAHARQPVK
                     LNGAYGGLPSGRGGATHSAVEDIAVMRTMPNMIVLTPADAAEAGAMVELAMETSAPVY
                     LRTMRCELPDLFGVGWRPDIGRAVELAPGDDVAILSEGMMSARALEAAAILRRDGIAA
                     RVLHFGTIKPFDAEAVAKAARECGALVSVENHSRIGGLGSAVCEAAAESDPAVVVRLG
                     FPDCFLESGDDERIFSRYGLDPAGIAAGAREALRRNGSDVSIRRAT"
     gene            complement(3581..4435)
                     /locus_tag="PLQ29_04955"
     CDS             complement(3581..4435)
                     /locus_tag="PLQ29_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPG86028.1"
                     /translation="MNESCYLDGQASLEGLTVLAGRARRLILEMTHRAGMGHTGGSLS
                     EVELLVALYFGGMRSIDPSRPDRPDRDRFILSKGHATPGYYSVLALRGYFSPELLETF
                     DELGTPLQAHPDMHKLPGVDMSSGSLGQGLSCGIGMAMAAMADPSLGSFRTFVLMGDG
                     ELQEGQVWEAAGYAGALRLPGIVAIVDCNAVQLSTATGPFQAPDAVAARFRAFGWDAS
                     GVDGHDLAALMPAIASARERAGRGPVVLVATTVKGKGVSFMEGRCEWHGKAPDDGELA
                     RALAELGS"
     gene            4523..>5047
                     /locus_tag="PLQ29_04960"
     CDS             4523..>5047
                     /locus_tag="PLQ29_04960"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPG86029.1"
                     /translation="MPKTDGAMIQSVERALALLERIADHPEASFSLTDLTEYMSLDKS
                     SVFRLLSTLGKYGLVRQEESRKGYLLGFGIYGLAASLRQQLKITELASPVLKRLAFAT
                     KENAHLAVRSGRLAVFIDRERAAKTIAANTDIGETEELYCTAVGRCLICRMDAKELGE
                     LFAGTELTRYTEATI"
BASE COUNT          896 a         1743 c         1667 g          741 t
ORIGIN      
        1 cgagtacatg caggtcggag atacggccgc agccggcgaa gacgaccctc aacgcgtcca
       61 tcccatcctc ctaccatccg agccgatgct tctcgcactg cactcgctcg caccaggcgc
      121 cgacgttcca atcgccgtaa gcgccgaggc caccctcgtc gtcgctcccg gcgtagacgg
      181 gcacatggat caccgacaga ccggtatgcg cgtaggccgc ttccagggcc gccctcagct
      241 ccagaaccga gtacccgcca tgcacgccca ggacgccctc gaccgacgag gccatcgcgg
      301 cgtagtcgac gacagcgcgg tcgtcggtgc gatagtcaac acagtactgc gctcgctgta
      361 gcgagctgat cgcggccatc ctccgattgt cgaagacgac gaccatgccg cgtaggccgt
      421 actcgaccgc gtcgatgata acctgattgc ccatcatgaa gctcccgtcg cccgtaaagg
      481 ctatcggata ggacggcgag tccgccaggg ccgaggcgag gatgccggac gcggcgaacc
      541 ccatgtacga ggcgccggtc tccgtaaagg tcgcccccaa cgtctcgtcc tcgacgagct
      601 ggaagccgtt ggcctgaaca tcgccggcat cgaagtactt gaccgcgccc ttctcccggg
      661 cgaacgcggc taccaccgct atggcggccg gctgtgtcat cgcctcacga cccaatttcg
      721 agtcgtacag cttctcgcga gctaggcgcc cggcgatgag ggcgtcccat tcggccctac
      781 gctcgcggca ctcgccgtac catcgcgtgg gcgaggagcc tttgtcgacg ccggcgtcgc
      841 ggagcgcctg gacaagccgt tcgaccagga cggcggcgtc gccgacgagg ggcaagctac
      901 ggttgtagtg cgctgcgtcc tcgggccttg cattgatcgc gatgaccgac ctcgccgagg
      961 cccaggccgt gcccgacgag tcccactggc agacggccct agccccgacg acgatggcca
     1021 gctccgcccg ggccatcgcc gcattacccg agatcgagcc ctttgagcct ccgacgccca
     1081 tgttacgcgg gtgggagccg ggcaagacgc cgacggccga cggcccgtgc acgtaagccg
     1141 cgtccgcccg ccgtagcagt tcgtcgagga gacgggggtc gactccgcgg gccccgttgc
     1201 ccgccttgag cgtcacacgc tccgccgcgg ctatcatgcg agcagcctcg acgaagggag
     1261 cgtcgtcagc cggcgcgagt cgctggaagg acgggcgccg agggagctcc tcgaggttga
     1321 acgaagggag gacggcggcc tgggcgttca tcggtaggaa caggtagaac ggcgcagcgc
     1381 gatccgcggc gaagaccgtc gtcgcgccgc gcttgagggc ggagggcagg gcccaagggt
     1441 cgaggaggct gtacgcggcg cccatagccg aggcggcctt gaggaagcaa acctgttcct
     1501 ggcgcgggat ctgctgcatg ttatagccct cgtcctggct cgtctcgtcc ccgaacaggt
     1561 agtagagccc gagcccgttc gaggccgggg ctatcgacgc cgccaaagcc tgcagggcgc
     1621 ccggacctat cgaagtaaag accgcggcgg tctcgccgta ctgccacctg agcgcggcgg
     1681 ccacgtgcga cgcctcgatc tcgctacgga cgttgaacgc ccgcacgagg ccggcagcct
     1741 cgtagatcct gagagcctcg cctacggccg tcgagccgtg tccgaagacg ccgacgtact
     1801 tatgtacgcc ctggcgcaac agccccagca cgagggcctc ggccagcggc acgtcttggt
     1861 cgcgttgtaa cgcccccgag gcgagcgcct cgtcaagccc gccggccctc gcgatggcgg
     1921 ctgcccttgc ggccgcgccg gcgtagcctg gcatagagcg gctcacgagg ctcttcacag
     1981 cgggactcac gcctcggccc tcgccgccat cgcggcggcc agggcctcat ccttcggccg
     2041 cccggtcagc gccgcttcca agaagcccgg atagcgtaga gcggggaggg ccacctcgtc
     2101 gggcagggcc gacacgagct cggccatcgg gcgatgagtc aggttcgaca gccgcccgag
     2161 cagctcgcgc gaggcgcgct ggggcatgct acggtgttcc ggatagcccg cgccgcgctc
     2221 cggcgtgaac aactgctcga acacgaggcg gagattgacg tcgccggccc agccgtagcc
     2281 ctggttcaag gccaggctta cgcagttacc gccgttgatg cgggcgaaca gccacgcgtc
     2341 aaggggacag aggacgtggc cgcaggacac gcccgggtag aggagggccg cgttcaggaa
     2401 gccctggccc gtgccgcagc cgccgatgac gaaatcgact cggccaaggc ccaggagcag
     2461 ggccgccatc aggcccgtat ggaggtagga tagctcgggc gcctcgcccg agcggcgcat
     2521 gccgcagttc aggagctcat acccgtgcgg ctcgagggcg cgaagaatat cagcgttccg
     2581 gtcggccgcg ctcgtctcgc tcagtatggc tagcctcatg tcgccctcct aatagagacg
     2641 tcggaaccgt tccggcgtag cgcttccctg gcgccggccg caatgccggc cgggtcgagc
     2701 ccgtaccgag agaaaatgcg ctcgtcatcg cccgattcca agaaacagtc cgggaagccc
     2761 agccggacga cgacggcggg gtccgactcc gccgccgcct cgcagacggc ggagccgagg
     2821 ccgccgatcc gcgaatggtt ctcgacgctg acgagcgccc cgcactcgcg ggcggccttc
     2881 gccacggcct cggcgtcgaa cggcttgatg gtaccgaaat ggagcaccct cgcggcgatg
     2941 ccgtcacggc gcaggatggc cgcggcctcg agggcgcgcg cgctcatcat cccctccgat
     3001 aggatagcca catcgtcgcc cggcgccagc tccaccgcgc gacctatatc ggggcgccaa
     3061 ccgacgccga acaggtcggg cagctcgcaa cgcatcgtcc gcaagtagac cggggcggag
     3121 gtttccatcg ccagctcgac catcgctccg gcctcggccg cgtcggcggg cgtcagcacg
     3181 atcatgttcg gcatcgtccg catcacggct atatcctcga cggccgagtg agtcgcccca
     3241 ccccggcccg aaggaaggcc gccatacgcc ccgttgagct tgaccggctg gcgggcatgg
     3301 gcgaccgaga cgcggacctg gtccgcggcc ctggtcgcca ggaagacggc gaaggcggag
     3361 acccagggca cgaagccgca gctcgccagg ccggcggcga cgcagacggc gttggcctcg
     3421 gctatgccca tcggatagaa gcggtcggga taggcggcgc ggaacagcga ggtctgcgtc
     3481 gagggacaga cgtccgggtc gaggacgacc attttcggat accgctccgc cagctcgacg
     3541 agggcccgtc cgaaggcggc ccgttgcgcg acgccggcgc tcatgacccg agctccgcca
     3601 gcgcgcgggc aagctcgccg tcgtcgggcg ccttgccgtg ccattcgcag cgtccttcca
     3661 tgaacgagac gcccttgccc tttacggtcg tagcgaccag gacgacggga ccgcgcccgg
     3721 ctcgctcgcg cgccgacgcg atagccggca tgagggcggc cagatcgtgg ccatccactc
     3781 ccgaggcgtc ccagccgaag gcccgaaagc gagcggcgac ggcgtcgggg gcctggaagg
     3841 ggccggtggc cgtagatagc tgcacggcgt tacagtcgac gatggcgacg atgccgggca
     3901 ggcgcaaggc tccggcgtaa ccggcggctt cccagacctg gccttcctgg agctcgccgt
     3961 cgcccatcag cacgaaggtc cggaaggatc caagcgacgg gtcggccatc gcggccatcg
     4021 ccataccgat gccacaggag aggccctggc ccagggagcc ggaggacata tcgacgccgg
     4081 gcagcttatg catatccggg tgagcctgga gcggggtacc cagttcatcg aaggtctcca
     4141 ggagctccgg cgagaaatag ccgcggagcg ccagcaccga gtaatacccg ggcgtggcgt
     4201 gccccttgga caggatgaag cggtcccgat caggcctgtc aggccttgag ggatcgatgg
     4261 aacgcatccc gccgaagtac agcgccacga gtagctcgac ctcggacagg gatccaccag
     4321 tatggcccat gccggctcga tgggtcatct cgagtatcaa acgccgcgcc cgtccggcga
     4381 ggaccgttaa accctcgagc gaagcctgcc cgtccagata acatgactcg ttcatattgt
     4441 tttatataca acccggttgc gtgcttgtca atgtttattt atatcgcttg tcacttcatg
     4501 cgctgacgac tatcatcggc tcatgcccaa gaccgatggc gccatgatcc agtccgtgga
     4561 gcgggcgctc gcccttctcg agcgcatcgc ggatcatccc gaggcttcgt tcagcttaac
     4621 ggatctcacc gagtacatgt cgctcgacaa gagctcggtg ttcaggctcc tatccaccct
     4681 gggcaagtat gggctggtca ggcaggaaga gagtcgcaag ggctacctgc tcggcttcgg
     4741 catctacggc cttgcggcct cgctcaggca gcagctcaag atcaccgagc tcgcgtcgcc
     4801 cgtactcaag cgcctcgcct tcgcaacgaa ggagaacgcc cacctggccg tgcgttcggg
     4861 acgactagca gtattcatag accgagagcg cgccgccaag acgatcgcgg ccaacacgga
     4921 catcggcgaa accgaggaac tatactgtac tgccgtcggc cgatgcctca tctgccgtat
     4981 ggacgctaaa gaactcggag agctcttcgc ggggacagag ctgacacgct acaccgaggc
     5041 cacgatc
//
LOCUS       DAOPHV010000138         5043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_22168_length_5043_cov_3.101443, whole genome shotgun sequence.
ACCESSION   DAOPHV010000138 DAOPHV010000000
VERSION     DAOPHV010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22168_length_5043_cov_3.101443"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PLQ29_04965"
     CDS             <1..484
                     /locus_tag="PLQ29_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86030.1"
                     /translation="GFRLALKFTVSVLTAAFFAVTVRDIAALLRDRSASPVMMLPARF
                     RTAINAMIRAGTGSRALIFGAFVSGVVVSVLELACTGQVYFPAISAMVQAGVSGSGLL
                     YLTLYNLAFVLPLLAVLAASLLGLRQEALRSFFKRHLVASKAAQAVLFAGLSIAIWLA
                     "
     gene            547..1446
                     /locus_tag="PLQ29_04970"
     CDS             547..1446
                     /locus_tag="PLQ29_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86031.1"
                     /translation="MESIEERQRRTKRHLGDRYDGRRVRSLNPFYQITPYIMPTRVDA
                     HDYFEDRIEISHTEAWLRRQRDAGYPEMGYLHVFIAAYVRMISQKPRLNRFIAGKKIF
                     ARNEITFSLALKKRLHEDSPETTVKMRFNPEDTIYDVMRTVNEAIGQNKETAATNATD
                     KVARLIMLCPGFLVSMLIGLLRVLDFYGIMPRVIHRASPFHTSVFITDLGSLGIKPIY
                     HHLYNFGTTSIFVAFGAKERQREILKDGTIVERRYIGMKVVNDERICDGHYYASAFKL
                     LLSYFKDPSQLERPPEAVVRDQD"
     gene            complement(1443..1817)
                     /locus_tag="PLQ29_04975"
     CDS             complement(1443..1817)
                     /locus_tag="PLQ29_04975"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HPG86032.1"
                     /translation="MKIAYLLVVLVVAILAVLFAAQNSAVVAISFFSWSTSGSLSLVL
                     ILTLSIGILIGVLIMAPSVFRRAFQSSGLRRRLKRLEKEKTALGDMAGAADAKATAKA
                     DEAPTEPTAPKKGKLTTGTDLG"
     gene            complement(1886..4078)
                     /locus_tag="PLQ29_04980"
     CDS             complement(1886..4078)
                     /locus_tag="PLQ29_04980"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG86033.1"
                     /translation="MQKGLGIRLRTLLVLGAIQLVILGGLVAVVTIRSRDAIASLSYY
                     STEYLSKSYAAEIERRLETAQTVASSASAAISGLQDAGLSRADSIDVLSRFVSVSDSL
                     FAAWAVFEPGAYGDADATYASSPGHDADGRLSPYWSLEDGEPSLSSALGPSGGASAPS
                     FYAEPGRFGATRFSAPHIRMQGQRQVTLISASVPIVSRGSVVGVAGVDLNLASLSRLV
                     QTLRPFDSGKAFLADASGRILAHYDAALTGSTIASIYGDDMMAEAAAATGRAQSFSTL
                     ASDEKGLPVYVLMTPVRLGSSGQAWSLGTAIPMSILLVAAKNLMLSVLTISITALAIL
                     AVSLWLSLGPILKPLKAAGRAIRDIAEGDADLTRSIELKRDDEVGELVNDFNRFIAKL
                     RAIVVNLKQAQSQLVSIGDSMAASSIESSSATSQILANIEGVRKQTVHQSGSVENASS
                     AIEEVAKNIESLDRMVATQAAGVTEASAAIEQMLGNIGAVTATIGRMAERFEGLRLSS
                     EEGKEKQSAVEAIVREISGQSELLLDANEAIASIASQTNLLAMNAAIEAAHAGDAGKG
                     FSVVADEIRSLAETAAEQSRGIGSELAKVKASIEAVVDASGASAASFGTVAQGIESLG
                     SLVKEIEGAMLEQKAGSNQILESLGEMNSITSEVQAGAREMTAGNEQVIGAMRELAEI
                     SQTIAGSMDEMAAGAQEIGTSAQDVSEIAERTRDSIRAMEDEIGRFKV"
     gene            complement(4174..>5043)
                     /locus_tag="PLQ29_04985"
     CDS             complement(4174..>5043)
                     /locus_tag="PLQ29_04985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG86034.1"
                     /translation="KIKQYNPDICICTHGMPAEVISHLIMENKLQTNLGIVVTDYYVH
                     AFWLTDVFTRFFVAKDESKAQLTRLGLPSDRVVVSGIPVLRAFAEKADKGELRARYGY
                     DVSKPLVLLSAGAFGVMTGDDIMQILDQIKTPCHMIVVCGSNKRLKDSLDAYIVKNKG
                     ANASDYTILGYTDAMHDYLRMADVFIGKPGGLSTSECLAAGVPMIIWDPIPGQEVYNT
                     YHVLEHGAGVMPNNAITIGYKVDRILSDPERQKRMSQAALALARPDAARVIVDEMLAH
                     EDETPVKAFKKLV"
BASE COUNT          875 a         1580 c         1579 g         1009 t
ORIGIN      
        1 aggattccgc ctggccctca agtttaccgt cagcgtgctg accgcggcct tcttcgccgt
       61 gaccgtcagg gatatcgcag cgctactgcg ggaccgctcg gcttcgccgg tcatgatgct
      121 gcccgcccgg ttccgcacgg ccatcaatgc gatgatccgc gccggaacgg ggagccgcgc
      181 gctgatcttc ggcgcgttcg tctcgggagt cgtcgtctcg gtgctcgagc tcgcctgtac
      241 cggtcaggta tatttcccgg ccatatcggc catggtccag gcgggagtct cgggctccgg
      301 cctcctgtac ctgaccctgt acaacctggc gttcgtcttg cccctcctgg cggtcctcgc
      361 cgcctcgctc ctgggcctgc gccaggaagc gctgagatct ttcttcaaga ggcatctcgt
      421 cgcgtccaag gccgcccagg ctgtgttgtt cgcggggctt tctatcgcga tatggctggc
      481 ctgaccaagg tcgccatcta gtaaacgagc ctccgagcga gtagaatggc ctctttcgga
      541 ggatcgatgg aatctattga agagcgccaa agacggacca agcggcacct cggcgaccga
      601 tacgacggca ggagggttcg ttccctcaat ccgttctacc agatcacgcc ctatatcatg
      661 cccaccaggg tcgacgccca tgattatttc gaagatagga ttgagatatc gcacaccgag
      721 gcctggctga ggcgccagcg cgacgccgga tatccagaga tgggctatct gcatgtcttt
      781 atcgcggcct acgtgcgcat gatctcccag aagccgaggc tcaaccgctt catagccggc
      841 aagaagattt tcgcgcgcaa cgagatcacc ttctcgctgg ctctcaagaa gcggctccac
      901 gaggacagcc cggaaaccac ggtaaagatg cgcttcaacc cagaagacac catctatgac
      961 gtgatgcgga ccgtcaatga ggcgataggt cagaacaagg aaaccgccgc gactaacgcc
     1021 accgacaagg tcgccaggtt gatcatgctc tgccctggct tcctcgttag catgctgata
     1081 ggacttttac gcgtcctgga tttctacggg atcatgccca gggtaatcca ccgggccagc
     1141 ccattccata ccagcgtatt cataaccgac ctgggctcgc tcggtatcaa gccaatctac
     1201 catcacctgt acaatttcgg caccacgtct atcttcgtcg ccttcggagc taaggagagg
     1261 caacgagaga tcctgaaaga tggtacgatc gtagagcgcc gctacatcgg catgaaggtc
     1321 gtcaacgacg agcgcatctg cgacggccat tactacgcca gcgcgttcaa gctgctccta
     1381 agctacttca aggacccgag ccagcttgag cgaccgcccg aggcggtcgt cagggaccag
     1441 gattagccca ggtcggttcc ggttgtaagc ttccctttct ttggagccgt gggctcggtc
     1501 ggcgcctcgt cagccttcgc ggtagccttc gcgtcagccg cgcccgccat gtcgccgagc
     1561 gcggtcttct ccttctccag ccgcttgagc cggcgcctga gccccgatga ctggaacgcg
     1621 cgcctgaaga ccgatggcgc catgatcagc acccctatga ggatgcctat cgataacgtc
     1681 agtatgagca cgagggacag ggatccggag gtggaccacg agaaaaagct gatcgcgacc
     1741 accgcgctat tctgggccgc gaataggacc gcgagtatag ctaccacgag tacgaccagc
     1801 aggtacgcta tcttcatact atctccttag ctcctcgtca tgcgccccgc gggcgtatga
     1861 attctatgta ctgttcggcg tgcatctaaa ccttgaagcg acctatctcg tcctccatcg
     1921 cgcggatgct gtccctcgta cgctcggcta tctccgagac gtcctgggct gacgttccta
     1981 tctcctgggc gccggccgcc atctcgtcca tgctgccggc tatggtctgg gatatctcgg
     2041 cgagttcgcg catggccccg atcacctgct cgttgcccgc ggtcatctcg cgggccccgg
     2101 cctgaacctc gctggtgatg ctgttcatct cgcctagcga ctcgaggatc tggttcgagc
     2161 ctgctttctg ctcgagcatc gccccctcga tctctttgac caggctgccc agcgactcga
     2221 tgccctgggc gaccgtgccg aagctggccg ccgaggcccc ggacgcgtcc acgaccgcct
     2281 cgatcgacgc cttgaccttg gccaattccg agccgatgcc cctggactgt tccgcggccg
     2341 tctccgcgag cgatcgtatc tcgtcagcga cgaccgagaa tcccttgccg gcgtcgccgg
     2401 cgtgagcggc ctcgatcgcg gcgttcatcg cgagcaggtt cgtctgagag gctatcgagg
     2461 cgatggcctc gttcgcatcg aggagcagct cggattgccc ggaaatctcg cggactatgg
     2521 cctcgacggc cgactgcttc tccttgcctt cctccgacga gaggcgcagg ccctcgaagc
     2581 gctcggccat cctgccgatg gtcgcggtca cagcaccgat gttcccgagc atctgttcga
     2641 tggcggccga ggcctccgtg acgccggcgg cctgggtggc caccatccgg tccagcgact
     2701 cgatgttctt ggcgacctcc tcgatggcgc tggaggcgtt ctcgacgctg ccgctctggt
     2761 ggacggtctg cttgcgaacg ccttcgatat tggcgaggat ctgtgaggtg gcgctcgatg
     2821 actcgatgga cgaggcggcc atgctatcgc cgatcgagac gagctggctc tgcgcctgct
     2881 tgaggttgac gacgatggcg cgcagcttcg ctatgaagcg gttaaaatcg ttgaccagct
     2941 cgcctacctc gtcgtcgcgc ttaagctcga tcgatcgggt aaggtcggcg tcgccctcgg
     3001 ctatgtccct gatcgccctg cccgcggcct tgagcggttt caggatgggg ccgagcgata
     3061 gccatagcga tacggccagg atggccaggg cggttatgct gatggtaagg acagacagca
     3121 tcaggttctt cgcggccacg aggaggatgg acatcggtat ggctgtgccg aggctccagg
     3181 cctgtccgga cgagccgagc cgtaccggcg tcatcagtac gtatacgggc agacccttct
     3241 cgtcggaggc gagtgtcgag aaggattggg cgcgcccggt agcggctgcg gcctcggcca
     3301 tcatatcgtc gccgtagatg gaagctatgg tcgaaccggt cagggcggcg tcgtaatggg
     3361 ccagtatccg gccggacgcg tcggcgagga aggccttgcc cgagtcgaac ggccgcaggg
     3421 tctgcaccaa tctggatagc gacgcgaggt tcaggtcaac gccggcgacg ccgacgaccg
     3481 agccgcggct gactatgggt acggaggccg agatgagcgt gacctgtcgc tggccctgca
     3541 tacggatatg aggcgcgctg aagcgagtcg cgccgaagcg cccgggctcg gcgtagaatg
     3601 acggagcgct cgcgccgccg gacggtccga gcgcggacga tagcgacggc tccccgtcct
     3661 cgaggctcca gtacggtgat agcctgccgt cggcatcgtg gccgggagac gacgcgtagg
     3721 tagcgtccgc gtcaccatag gcgccgggct cgaagaccgc ccaggccgcg aatagggagt
     3781 cgctcaccga gacgaaacgg gatagcacgt ctatcgagtc cgccctcgat aggccggcgt
     3841 cctgtaggcc cgatatcgcc gccgaggcgc tcgaagcgac ggtctgggcg gtctcgagcc
     3901 ggcgctcgat ctcggcggcg taactcttgg agaggtactc ggtcgaatag tacgagaggg
     3961 acgcgatcgc gtctctcgac cggatcgtca cgacggccac gagcccgccg agaatgacga
     4021 gctggatggc cccgaggacg agcagcgtcc tgagccgtat gcctagtccc ttttgcatcg
     4081 atcccctttc agtcgtcacg ggaaagcgcc cgctcgtcgt tcgcccggtt tggacgtgcc
     4141 tgacctgaga gggggcccat cgcccgcgat agcttatacg agtttcttga acgccttgac
     4201 cggcgtctcg tcctcatggg cgagcatctc gtctacgatc acacgggccg cgtccggcct
     4261 ggccagggcc agcgcggctt ggctcatgcg cttctggcgt tccgggtcgg acaggatacg
     4321 gtcgaccttg tatcctatcg taatggcgtt gttcggcatg acgccggccc cgtgttccag
     4381 gacatggtag gtattataga cctcctggcc ggggatgggg tcccaaatga tcatgggtac
     4441 gccggcggcg aggcattcgc tcgtcgaaag ccccccgggt ttgccgatga atacgtcggc
     4501 catcctcagg tagtcgtgca tcgcgtcggt atagccgagt atggtgtagt cggaggcgtt
     4561 cgcgcccttg ttcttgacta tgtacgcgtc aagcgagtcc ttcagtcgct tgttgctacc
     4621 gcagacgact atcatgtggc agggcgtctt gatctggtcc aggatctgca tgatgtcgtc
     4681 tccggtcatc acgccgaagg ctccggccga taggagtacg agcggcttgc tcacatcgta
     4741 gccgtagcgg gcgcgcagct cgcctttgtc ggccttctcc gcgaaggctc ggagtaccgg
     4801 gatgcctgat acgacgacgc ggtcggaggg caggcctagc ctggtcaact gcgccttgct
     4861 ctcgtccttg gcgacgaaga agcgcgtgaa cacgtctgtc aaccagaacg cgtgcacgta
     4921 gtagtcggtg acgacgatgc cgaggttggt ctggagcttg ttctccatga tcaggtggga
     4981 gatgacctcc gcgggcatgc cgtgggtgca gatgcagatg tcggggttgt attgcttgat
     5041 ctt
//
LOCUS       DAOPHV010000139         4972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_22613_length_4972_cov_4.342282, whole genome shotgun sequence.
ACCESSION   DAOPHV010000139 DAOPHV010000000
VERSION     DAOPHV010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22613_length_4972_cov_4.342282"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="PLQ29_04990"
     CDS             complement(<1..1182)
                     /locus_tag="PLQ29_04990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG86035.1"
                     /translation="MENKPAAPSPRRRRRTRIERYRSRFLAFNVLNSFSFMLLSGSIP
                     TLFALDLGASGTYIGLLGSLNFITYFFMPLGRRAIRGRPIIRVFGWSWMLRYWVMAPA
                     IAAPFVAMAGYPSIGLALLLLGSLAFNVFRGVGLIGNNPVLGMMAGERDRGAFLSSVQ
                     ITNGLSAIGTSALTMLALALWSGSPLYAGILALGVASGSIASAILLSLPEPEAYRPDP
                     GSSFAATVREAWADPRLRRFVAVFAPLSFAGGTARTFVVTHASVLYGQSPALVMAYSV
                     AFNLGGVAVGYLSRKLMDRLGAKPLYVLFAVAMALAMAPVAWSPATPSALSAAAYLAL
                     VSFIVGAGVAGQENAGQTYFFSIVKPSHMLDLGVLYYVIFGVGGALGSTGNPVGPVPT
                     AR"
     gene            complement(1215..2576)
                     /locus_tag="PLQ29_04995"
     CDS             complement(1215..2576)
                     /locus_tag="PLQ29_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPG86036.1"
                     /translation="MKDLTQGGETRTIIAFALPMLIGNVFQQFYNMVDSIVVGNFVGT
                     TALAAVGTSFPVIFLMISLVMGLTMGTTVLVSQYFGAKDLAKVRAAVDTGYIILFWSG
                     LAMSIIGVLSTNAILTALKVPADVFDEAATYLRIIFGGMLATFGYNAISAVLRGLGDS
                     KTPLYLLVGASLLNIALDLLFVVVFGWGVAGVAWATIISQGASFVGGLVYVAKRNPLV
                     SLKPRSLGFDRDVFKHSVRIGLPTGIQQTMVALGMMALTRLVNGQGTSTIAAFAAASR
                     LDTFASMPAMNLSQALSTFTGQNMGAGKTERVKRGHLSASLVGAAISVAVGVAVILFG
                     RGLMGMFTRDAEVMAIGARYLAIVGATYLLFSTMFINNGVMRGAGDAFIPMINTVLAL
                     WVVRIPCAVLFSGPLGMGSDGIWWSVPAGWLMGAVFSTWYYLGGRWKRKAVVRAGPEV
                     AGR"
     gene            2682..3500
                     /locus_tag="PLQ29_05000"
     CDS             2682..3500
                     /locus_tag="PLQ29_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86037.1"
                     /translation="MNIPFLKLRVADCPWIVFDRSTTMTGPSAPTSADDDRPWPAVAR
                     AICDRHRGAAAVGVVAIEGDGGRLGVRTWDADGEERSPSPAALLCASRLLFDAGRAGA
                     ESLELEGGGGTAEILVLDSRSFGLSVGLPATDDGQPLDRRYGSGGLGLAAAPGAGVAM
                     SLRVGGERVEVALYDGPRGVRGGARRGRSESARVDAIAIARHELRMRRGSADPILAAA
                     ASTAAAVVADYADRETVVVVGGDRLVVQWPEGGPVFVATAPEYCLSGEFWLPEE"
     gene            complement(3506..4240)
                     /locus_tag="PLQ29_05005"
     CDS             complement(3506..4240)
                     /locus_tag="PLQ29_05005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG86038.1"
                     /translation="MAVLLPAGGSAFPVLGGSYEGGGDDAAEALGGLIRSRAVRTYRP
                     AACVGASDHVAALEAALGWRPALEVEYDAMRLLPEARPAGGRRLDGGRRLAAHGFRSG
                     PAEIRRAGPDDLDALYPIAAAYEQAEVMTELHAFDPEACRAAQARSLASQLVYIAIVG
                     GRPVARAQTNARGLATDQIGGVYVEPERRGLGLGRLVVEALVAAILSDGRAASLFVKK
                     TNAIARSLYLSMGFACARDYRVSYFA"
     gene            complement(4432..>4972)
                     /locus_tag="PLQ29_05010"
     CDS             complement(4432..>4972)
                     /locus_tag="PLQ29_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86039.1"
                     /translation="LPLGRDGVRPDDAPEAAMRSEESRAFFAERAMRSATVLGRSELP
                     LPETARVLVAGPFGDFTAEASARVPRATLYRFSYQPSADGLASELASFRARLPAADAV
                     VVCVANPEGAAFARAARKAGKRLYVVSALNPAYAMEFKDDATVVAVYSYARESFKAAF
                     AVLTGDIEAIGRLPIEAGS"
BASE COUNT          823 a         1666 c         1777 g          706 t
ORIGIN      
        1 cctggccgtg gggacaggtc cgacggggtt gccggtcgat ccgagcgcgc cgcctacgcc
       61 gaagataaca taatagagga cgccgaggtc gagcatgtgg ctcggcttga cgatggagaa
      121 gaaataggtc tggccggcgt tctcctggcc cgccaccccg gctccgacga tgaagctgac
      181 cagggccagg taggcggcgg cgctcagggc gctaggcgtc gccgggctcc aggccaccgg
      241 cgccatcgcc agggccatcg ccacggcgaa gagtacgtac aggggcttgg ctccgagccg
      301 gtccatgagc ttgcgcgaca ggtagccgac cgcgacgccg ccgaggttga aggcgaccga
      361 gtaggccatg acgagggccg ggctctggcc gtagagcacg ctggcgtggg tgacgacgaa
      421 ggtgcgggcg gtgccgccgg cgaacgacag cggggcgaac accgcgacga agcgcctgag
      481 ccgcgggtcc gcccaggcct cgcgcacggt cgccgcgaac gacgatccgg ggtcgggcct
      541 gtaggcctcc ggctcgggca gcgacaggag tatcgccgag gcgatcgagc cggaggccac
      601 gccgagcgcc aggatgccgg cgtacaaggg gcttccagac cagagtgcga gcgccagcat
      661 ggtcagcgcg ctcgtgccta tggccgataa gccgttcgtg atctggacgc tcgacaggaa
      721 ggccccgcgg tcgcgctctc ccgccatcat gccgagcacc gggttgttgc ctatcaaccc
      781 gacgcccctg aagacgttga aggcgaggct accgagcagc agcagggcga gccctattga
      841 tgggtagccg gccatggcca cgaagggggc cgctatcgcc ggcgccatga cccagtaacg
      901 cagcatccat gaccagccga acaccctgat gatgggcctg ccgcgtatgg cgcgcctgcc
      961 gagcggcatg aagaagtagg taatgaagtt gagcgacccg agcaggccta tgtaggttcc
     1021 ggacgctcca agatccaacg cgaacagcgt cggtatactg cccgatagca gcatgaacga
     1081 gaacgaattg agcacattga aggccaggaa acgggagcgg tagcgctcga tcctggtcct
     1141 tcgcctccgt cgcggcgaag gggccgcggg cttattttcc atggatagag tatagagcgt
     1201 cctaagcgga taggctatct ccccgcgacc tcgggacccg cccgtaccac ggcctttcgc
     1261 ttccagcggc cgcccaggta gtaccaggtc gagaacaccg cgcccatcag ccagccggcg
     1321 ggcacgctcc accagatgcc gtccgagccc atgccgaggg ggccggagaa cagtacggcg
     1381 caggggatac gcacgaccca gagggcgagc accgtgttga tcatcggtat gaacgcgtcg
     1441 ccggcgccgc gcatgacgcc gttgttgatg aacatcgtcg agaacagcag gtaggtagcg
     1501 ccgactatgg ccaggtaacg ggcgccgatc gccatcacct cggcgtcgcg ggtgaacatg
     1561 cccatcagac cgcgcccgaa taggatgacc gcgacgccca cggccacgga gatcgcggcg
     1621 ccgaccaggc tggccgagag gtggccgcgc ttgacccgtt cggtcttgcc ggcccccatg
     1681 ttctggcccg tgaaggtgga caaggcttgg ctcaggttca tcgcgggcat cgaggcgaag
     1741 gtgtccagcc tggaagccgc ggcgaaggcg gcaatcgtcg acgtgccctg cccgttgacc
     1801 agcctggtca gggccatcat gccgagcgcc accatggtct gctggatgcc ggtaggcagg
     1861 ccgatgcgca cgctgtgctt gaatacgtcc cggtcgaagc ccaggctcct cggtttcagg
     1921 ctgaccaagg ggttccgctt ggccacgtag acgagaccgc cgacgaagga ggcgccctgc
     1981 gagatgatgg tggcccaggc cacgccggcg acgccccagc cgaagacgac cacgaacagc
     2041 aggtcgagcg ctatgttgag caggctggcg cccacgagca ggtacaaagg cgtcttggag
     2101 tcgccgaggc cgcgcaggac ggccgagatg gcattgtagc caaaggttgc cagcatgccg
     2161 ccgaagatga tgcgcaggta ggtcgcggcc tcgtcgaaca cgtcggcggg caccttgagg
     2221 gcggtcagga tggcgttcgt cgatagcacg ccgatgatcg acatggccag gcccgaccag
     2281 aacaggatga tgtagccggt gtcgacggcg gcccgcacct tggccaggtc cttggcgccg
     2341 aagtactggg acacgagtac cgtcgtcccc atggtcagcc ccatgaccag cgagatcatc
     2401 aggaagatga ccgggaacga ggttccgacc gccgcgaggg ccgtcgtccc gacgaagttg
     2461 ccgaccacga tactgtcgac catgttgtag aactgctgga acacgttgcc gatcagcatc
     2521 ggcagggcga acgcgatgat ggtccgcgtc tcgccgccct gggtcagatc cttcatgtaa
     2581 cctcgtcgtt tacggtctag cgataattgt aacgacggcg ctcccggtgg acaaggacgc
     2641 gggccttgat cgcctcgccc cggcgcgcta gggtagtcgt catgaatatc ccgtttctca
     2701 agctacgcgt agccgactgc ccgtggatcg tgttcgatag gtcgacgacg atgaccgggc
     2761 cgtcggcgcc gacctcggcc gacgacgacc ggccttggcc ggcggtcgcc cgggccatct
     2821 gcgaccgcca tcgcggcgcg gccgccgtgg gcgtcgtcgc gatcgagggc gacggcggcc
     2881 ggctgggcgt gcggacctgg gacgcggacg gcgaggaacg ctcgccgagt ccggccgcgc
     2941 tgctctgtgc gtcccgcctg ctgttcgacg cgggccgcgc cggcgccgag tccctggagc
     3001 tggagggcgg cggcgggacg gccgagatac tcgtgctcga ctcgaggagc ttcggcctgt
     3061 cggtcggcct gcccgccacc gacgacggcc agccactcga ccggcgctat ggttccggtg
     3121 gcctcggcct cgcggccgcc cccggcgccg gcgtggccat gtcgctgaga gtcggcgggg
     3181 agcgggtgga ggtggcgctg tatgacggcc cgaggggcgt tcggggcggt gcccggcgcg
     3241 gccggtcaga gtcggcgagg gtggacgcga tcgccatcgc caggcacgag ctacggatgc
     3301 gccgaggttc cgcagatccc atactggcgg ccgccgcctc gacggcggcc gccgtggtcg
     3361 ccgactacgc cgaccgggaa accgtcgtcg tcgtgggagg cgaccgcctc gtcgtccagt
     3421 ggcccgaggg cgggcccgtc ttcgtcgcta cggctccgga gtactgcctg tccggggaat
     3481 tctggctgcc cgaggaatga ccggttcagg cgaagtacga gactcggtag tcccgggcgc
     3541 aggcgaagcc catcgacagg tagagcgagc gggcgatggc gttcgtcttc ttgacgaaca
     3601 gcgaggccgc cctcccatcc gataggatag ccgcgacaag cgcctcgacg acgagcctcc
     3661 cgaggccgag tccccggcgc tccggttcga cgtagacgcc gccaatctgg tcggtcgcta
     3721 ggccgcgggc gttcgtctgg gccctggcta ccgggcggcc ccctacgatc gcgatataga
     3781 ctagctggct cgcaaggctc cgcgcctggg cggcgcggca ggcctcgggg tcgaaggcgt
     3841 gcagctcggt catcacctcg gcctgctcgt aggcggccgc gatggggtag agcgcgtcca
     3901 ggtcgtcggg cccggctcgt ctgatctcgg ccggaccgga tctgaagccg tgagccgcca
     3961 gccgccggcc gccgtccagc cgccgaccgc cggccggccg ggcttcgggc aagaggcgca
     4021 tcgcgtcgta ctcgacctcg agcgccgggc gccagcccag ggccgcctcg agggcggcca
     4081 cgtggtcgga cgccccgacg caggcggccg ggcggtaggt ccgtaccgcc cgcgagcgga
     4141 ttagcccgcc gagcgcctcc gcggcgtcgt cgccgccgcc ttcgtagctc ccgccgagca
     4201 ccgggaacgc cgagcctccg gccggcaaca gcaccgccat cgacaagcga tcggcgcggt
     4261 cctcgtcggg acgtacgaac aggccgcccc gatcgggacg gatacgagtc agctcgccat
     4321 gggccgagaa cgccttatac gagaatcctg tggcgtagcc ctcgcggggc tccaggaagc
     4381 gccggagggc gggcgcgtcg ccgtccgcgg ccctccgcca gccctcgggg ctcaggatcc
     4441 ggcctctatg ggcaggcgac ctatcgcctc tatgtctccc gtcagtaccg cgaaggccgc
     4501 cttaaacgat tctcgcgcgt aggaatagac cgccacgacc gtggcgtcgt ccttgaactc
     4561 catcgcgtaa gccgggttga gcgccgaaac gacgtagagc cgcttgcccg ccttgcgggc
     4621 cgcccgcgcg aaggccgcgc cctcggggtt ggccacgcag acgacgaccg cgtcggccgc
     4681 cgggagccga gccctgaacg acgccagctc cgaggccagt ccgtcagcgc tcggctggta
     4741 cgagaatctg tagagcgtag cccgaggcac gcgggcggac gcttcggcgg tgaagtctcc
     4801 gaaggggccg gcgaccagca cccgggccgt ctccgggagg ggtagctcgg agcggccgag
     4861 caccgtggcg ctgcgcatcg cccgctcggc gaagaacgcg cgtgattctt cgctgcgcat
     4921 cgcggcttcc ggggcgtcgt cgggcctgac cccgtctcgg ccgagtggca gg
//
LOCUS       DAOPHV010000140         4953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_22738_length_4953_cov_3.840343, whole genome shotgun sequence.
ACCESSION   DAOPHV010000140 DAOPHV010000000
VERSION     DAOPHV010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22738_length_4953_cov_3.840343"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLQ29_05015"
     CDS             complement(<1..346)
                     /locus_tag="PLQ29_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86040.1"
                     /translation="MSIQIADIGKAVIETQYAVRGPIVARAQELERAGRRIIYCNIGN
                     PQALGQAPLSWTRRLLALCEYPGLATAAPDAFPSDAVDAARALIAGSKHGVGAYSESK
                     GLKVVRDAVAAFI"
     gene            547..1815
                     /gene="hflX"
                     /locus_tag="PLQ29_05020"
     CDS             547..1815
                     /gene="hflX"
                     /locus_tag="PLQ29_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPG86041.1"
                     /translation="MSELHDTAARPERAFLVGVRDVEVRAPEAESLLRELSGLASSLG
                     IETVGSAMVGLRARVASTMLGSGKVEEIIAAAKAEGADSIIFDKALSPVQQRNWEQLS
                     GLDVYDRAELIIDIFASRAKTREASLQVELARLRYSLPRLAHSYGDLHRQRGGRYGTK
                     GAGEQKIELDRRQIAKRIADIEEELVVVRRSRATQRKRRERLGLKRAAIVGYTNAGKS
                     SLLNAIASTADVVAEDKLFATLDPTTRRLDTSSGSVLVTDTVGFVRNLPHGLVEAFKA
                     TLEEAADADLLVHVVDASDPEAAAQFATTMKVLAEIGATAETLLVLNKIDRLADRSVP
                     SLPFEGVSVLTSAVTGEGLPALVASIEKALTADALELTLRVPPEDYAVVPLLYREATV
                     LSEDHDGEATIVRCRVPERLGSRVEKYRVD"
     gene            complement(1875..3443)
                     /locus_tag="PLQ29_05025"
     CDS             complement(1875..3443)
                     /locus_tag="PLQ29_05025"
                     /inference="COORDINATES: protein motif:HMM:NF036553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HPG86042.1"
                     /translation="MPTNNDPASRDDGDSSSGFFQRLLGLFGMSSPDADKKRLLKNLA
                     KDLSRSRYKFYRPKSGEALPALARFFYETYKVIAPAQLMLTNASQSGVLKSFVIESFL
                     TPEQRQMSECMTDAYIQEKAKTMNVKDLQEHVKQVMLSFFAAFDAEKTAQIDGAYSTL
                     LSFINFINFDFFFLLKKFDSNVAERNFSYNPKFDAISADYVADDLHDFLEVFLPLNLD
                     ADWKRIFTALKEYRSMDVIQVDTWVKLVPALRDVRESGVLESVVMHAKKDPTLTVAPR
                     AASERIVESFIEKSKNQTAMTLQKIAQERRNSKIDELAKAVFGTTIVLRMKNYTDKSN
                     VAFAKKMLGGYTQTQALNFLKAYLIDYFKKDIREIVDVLLIRGQWSTNVQSQQLSDGY
                     HALLEVSEGILQFDDSLADDGELGNKLRNVLGKADRDKDALKYVRQILKDVNEKALGL
                     VNKAALNLITVGRHLKALIDDLGKPHHELILNWKEVEGAATVPMKEAMTESYKKIYYM
                     VQLLQYYVKEESAH"
     gene            complement(3477..4223)
                     /locus_tag="PLQ29_05030"
     CDS             complement(3477..4223)
                     /locus_tag="PLQ29_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPG86043.1"
                     /translation="MSRLARFFVASTTSTMDEARRLSAGRERGVVSAGTQSAGRGRLA
                     GRTWVDEPGASLLATFWLPAADYSGAPPSLVAGLLVLEAVRAWVASAGARLGPEPRLK
                     WPNDLLVGGRKLAGVLCESSGGLVYAGVGVNCGQLAFPPGLRTEPTSLRLETGLAPAP
                     EALLDALAEAIADRSAFASGWKARYEAAMAWRGEPVEFRPGLDGEPILGTLVGVDATG
                     AALIAPDDVRRAPAAGSATAYASGELRRRY"
     gene            complement(4220..>4953)
                     /locus_tag="PLQ29_05035"
     CDS             complement(4220..>4953)
                     /locus_tag="PLQ29_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPG86044.1"
                     /translation="ERESFAVYYQARVDAATRRVESAEALVRWIDGDAVIPPSEFIPL
                     AEDNGLIVPIGEWVLARALSDLRSWLERDPSLRVSVNLSARQFRLPDIGERVDRALSS
                     AGVPYDRLELELTESVAMHDVARSVAVMSDLDRRGLSFSLDDFGTGYSSLYYLNRLPI
                     RWLKIDQSFVRDIRQPDASPSNAIVNTIIDMARSLGLGTIAEGCETEEQFGFLSRRGC
                     SQVQGYLFSRPAPAAEFEQLIGRAL"
BASE COUNT          770 a         1589 c         1757 g          837 t
ORIGIN      
        1 cgatgaaggc ggctacggcg tcgcggacga ccttgaggcc cttgctctcg gagtaggcgc
       61 cgacgccgtg cttggagccg gcgatcagcg ccctcgccgc gtcgacggcg tcggagggga
      121 aggcgtccgg agcggccgtc gcgagtccgg ggtactcgca gagggccagg agccgcctgg
      181 tccacgacag cggggcctgg ccgagggcct ggggattgcc gatgttgcag tagatgatcc
      241 tgcgcccggc ccgctctagc tcctgggccc tggcgacgat ggggccgcgc acggcgtact
      301 gcgtctcgat gactgccttg ccgatgtcgg ctatctggat ggacatgggg actccttttc
      361 gatcgcgcgg cgccgggccg cgcgcgccca taatagcatc gctcggggcg ggcgccaagg
      421 cggggcgtcg tatccggcgt atagtatcgc ctcggaaccg aagccgcgga gcgcgggccg
      481 cggagcgaac gggtatagcc gaactggccc tcgaccgagg gcggccttcg aggctagtat
      541 aggttcgtga gcgaattaca cgatacggcc gcccgtccgg aacgggcctt cctcgtcggc
      601 gtgcgcgacg tggaggtacg ggcgcccgag gccgagagcc tcctccgcga gctgtccggg
      661 ctggcctcga gcctcggcat cgagacggtc ggcagcgcca tggtcggcct gcgggcccgc
      721 gtcgcctcga cgatgctcgg ctcgggcaag gtcgaggaga tcatcgccgc cgccaaggcc
      781 gagggcgccg actccatcat cttcgacaag gcgctcagcc ccgtgcagca gcgcaattgg
      841 gagcaactat ctggcctgga cgtgtacgac cgggccgagc tgatcataga catcttcgcc
      901 agccgcgcga agacccggga ggcgtcgctc caggtggagc tggccaggct acgctactcg
      961 ctgcctcgcc tggcccactc ctacggcgac ctgcaccggc agcgaggcgg ccgctacggc
     1021 accaagggcg ccggcgagca gaagatcgag ctcgaccgcc ggcagatagc caagcgcata
     1081 gccgatatcg aggaggagct cgtggtcgtg cgccgctcga gggctacgca gcgtaagcgt
     1141 cgcgaacggc tcgggctcaa gcgggcggcc atcgtcggct ataccaacgc cggcaagtcg
     1201 tcgctgctca acgcgatagc ctcgaccgcc gacgtcgtcg ccgaggacaa gctgttcgcc
     1261 acgctggacc cgacgacgcg ccggctggac acctcgtcgg gctcggtgct ggtcaccgac
     1321 acggtcggct tcgtgcgcaa cctgccccac ggcctcgtcg aggccttcaa ggccacgctc
     1381 gaggaggccg ccgacgccga cctgctcgtc cacgtcgtcg acgcgtcgga cccggaggcc
     1441 gcggcgcagt tcgcgacgac gatgaaggtg ctcgccgaga taggcgcgac ggccgagacg
     1501 ctgctcgtcc tcaacaagat agaccggctg gcggaccgtt ccgtgccgag cctgccgttc
     1561 gagggcgtct cggtgctgac ctcggccgtg accggggagg ggctacccgc cctggtcgcc
     1621 tccatcgaga aggcgctgac cgccgacgcg ctcgaactga cgctgcgggt gccgcccgag
     1681 gactacgccg tggtgccgct cctgtaccgc gaggcgacgg tgctgtccga ggaccacgac
     1741 ggcgaggcga cgatcgtgcg ttgcagggtg cccgagcgcc tcggctctcg ggtggagaag
     1801 tacagggtcg actgatcgcg ccgctcaggc ggcgcgtcgg ctctcatgag ccacggcgct
     1861 aatgcgccac ggctctaatg agccgattct tctttgacgt agtactgaag cagctggacc
     1921 atatagtaga tcttcttata cgactcggtc atcgcttcct tcatcgggac ggtcgcggcg
     1981 ccctccacct ctttccagtt caggatcagc tcgtggtggg gcttgccgag gtcgtcgatc
     2041 agcgccttga ggtggcgccc gacggtgatc aggttgagcg cggccttgtt gacgaggccc
     2101 agggccttct cgttgacgtc cttgagtatc tgcctgacgt acttgagcgc gtccttgtcg
     2161 cggtcggcct tgcccagcac gttgcgtagc ttgttgccga gctcgccgtc gtcggccagg
     2221 ctgtcgtcga actggagtat gccctccgat acctcgagca gggcgtggta gccgtccgac
     2281 agctgctggg actggacgtt ggtcgaccac tggccgcgta tcagcaacac gtcgacgatc
     2341 tcgcggatgt ccttcttgaa gtagtcgatc aggtaggcct tgaggaagtt gagcgcttgg
     2401 gtctgggtgt agccgccgag catcttcttg gcgaaggcga cgttcgactt atcggtatag
     2461 ttcttcatgc gcagcacgat ggtcgtaccg aagaccgcct tggccagctc gtcgatcttg
     2521 gagttgcgcc gttcctgggc gatcttctgc agggtcatcg cggtctggtt cttggatttc
     2581 tcgatgaagc tctcgacgat gcgctccgac gcggcccggg gggccacggt cagcgtcggg
     2641 tccttcttgg cgtgcatcac gacgctctcg agcacgcccg actcccgcac gtcgcgcagg
     2701 gcggggacga gcttgaccca ggtgtcgacc tggatcacgt ccatcgagcg gtattccttg
     2761 agcgcggtga agatgcgctt ccagtcggcg tcgaggttga gcggcaggaa gacctcgagg
     2821 aagtcgtgca ggtcgtcggc cacgtagtcc gcggagatgg cgtcgaactt ggggttgtac
     2881 gagaagttgc gctcggccac gttcgagtcg aatttcttga gcaggaagaa gaagtcgaag
     2941 ttgatgaagt tgatgaagga caatagcgtg gagtaggcgc cgtctatctg ggccgtcttc
     3001 tccgcgtcga acgccgcgaa gaacgatagc atcacctgct tgacgtgctc ctggaggtcc
     3061 ttgacgttca tcgtcttggc cttctcctgg atgtacgcgt cggtcatgca ctccgacatc
     3121 tggcgctgct ccggcgtcag gaagctctcg atcacgaagg acttgagcac gccgctctgc
     3181 gacgcgttcg tcagcatcag ctgggcgggc gcgatgacct tgtaggtctc gtagaagaag
     3241 cgggcgagag cgggcagggc ttcgcctgac ttgggcctgt agaacttgta gcgggagcgc
     3301 gacaggtcct tggcgaggtt cttgagcagg cgcttcttgt cggcgtcggg gctactcatg
     3361 ccgaagagac ctagcagcct ctggaagaat ccagaggacg agtcgccgtc atcccggctg
     3421 gccgggtcgt tgttcgtggg catacgcgga aaccttacta tgtcctggtt tactcgtcaa
     3481 taacgacgac ggagctcgcc cgaggcgtac gcggtcgccg atcctgcggc cggggccctc
     3541 cgtacgtcgt caggggcgat cagcgcggcg ccggtagcgt cgacgccgac gagcgtcccg
     3601 aggatcggct ctccgtccag gccgggcctg aactcgaccg gctcgccgcg ccaggccatc
     3661 gcggcctcgt agcgggcctt ccagccgctc gcgaaggcgc tcctatccgc tatcgcctcg
     3721 gcgagcgcgt cgaggagcgc ctcgggggcg ggggccaggc cggtctcgag ccgcagcgag
     3781 gtcggctcgg tccggaggcc cggtgggaag gctagctggc cgcagttgac gccgacgccg
     3841 gcgtagacga gcccgcccga cgactcgcag agcactccgg cgagcttgcg gccgcctacg
     3901 aggaggtcgt tcggccactt gagcctgggt tcaggcccga ggcgggcgcc ggcggaggcg
     3961 acccaggcgc ggacggcctc cagcacgagc agcccggcga cgagggacgg cggcgcgcct
     4021 gagtagtcgg cggcgggtag ccagaacgtg gccaggaggc tggcgcccgg ctcgtcgacc
     4081 caggtccgcc cggccagccg gccgcgaccg gcgctctggg tgcccgcgct tacgacgccg
     4141 cgttcgcgcc cggccgatag gcggcgcgcc tcgtccatcg tgctggtcgt cgacgcgacg
     4201 aagaagcgcg cgaggcggct cacagcgcgc ggcctatcag ctgctcgaat tcggccgccg
     4261 gggccggcct cgagaagagg tagccctgga cctgggaaca gccgcgcctc gagaggaagc
     4321 cgaactgctc ctcggtctcg catccctccg ctatggtgcc gaggccgagg ctcctggcca
     4381 tgtcgatgat cgtgttgacg atggcgttcg acggcgaggc gtccggctgg cgtatgtcgc
     4441 gcacgaagga ctggtctatc ttgagccagc gtatgggcag gcggttcagg tagtagagcg
     4501 acgagtagcc ggtgccgaag tcgtcgaggc tgaaggatag gccgcggcgg tcgagatcgc
     4561 tcatcacggc gaccgagcgc gccacgtcgt gcatggcgac gctctcggtc agctcgagct
     4621 cgagcctgtc gtagggcacg cccgcagacg acagggcccg gtcgacgcgc tcgccgatgt
     4681 cgggtagcct gaactggcgg gcggacaggt tgaccgagac gcgcagcgac gggtctcgct
     4741 cgagccatga gcgcaggtcg gatagggccc gggcgagcac ccactcgcct ataggcacga
     4801 tcaggccgtt gtcctcggcg agcggtatga actccgacgg cggtatgacg gcgtcgccgt
     4861 cgatccagcg gaccagggcc tcggcgctct cgacccgcct ggtcgccgcg tcgacgcggg
     4921 cctggtagta cacggcgaac gactcgcgct cga
//
LOCUS       DAOPHV010000141         4932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_22884_length_4932_cov_4.914497, whole genome shotgun sequence.
ACCESSION   DAOPHV010000141 DAOPHV010000000
VERSION     DAOPHV010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22884_length_4932_cov_4.914497"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..637
                     /locus_tag="PLQ29_05040"
     CDS             38..637
                     /locus_tag="PLQ29_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPG86045.1"
                     /translation="MRTRTPLIYFREVQSHRYQAIWPVIVLEDVQERLCVRAAIDPAY
                     AELRPGLSFSDIAESPLDLRRYAWTQTRHRLHQGAFRELVVSAYEERCAICRLNHPEL
                     LDAAHIIPDSDERGTPIVANGLSLCKIHHAAYDQGILGIDADYRVHIREDILLERDGP
                     MLKHGIQELDGSTLIVPRHADERPDRDRLAERYSRFFVA"
     gene            complement(650..1060)
                     /locus_tag="PLQ29_05045"
     CDS             complement(650..1060)
                     /locus_tag="PLQ29_05045"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HPG86046.1"
                     /translation="MVCNAVGALPRLIVVAAVIELDGRILCAQRKAGGELSLKWEFPG
                     GKVEPGETRERALEREIDEELGLRILTGPLFLTVRHWYPAFAVTMHAYRATILEGRPE
                     LREHSAIRWLRPDELGTLDWAPADVPVAKKMIGL"
     gene            1164..1715
                     /locus_tag="PLQ29_05050"
     CDS             1164..1715
                     /locus_tag="PLQ29_05050"
                     /inference="COORDINATES: protein motif:HMM:NF018813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HPG86047.1"
                     /translation="MAIGATVCKVTLSVSDLDRHYYAEHRLTVSREPSEPDERFMARV
                     LAFALNADDGLAFARGQAEADEPELWRRGLDGGIGLWIELGLPDPKRLRKASARADEV
                     LLYLYHGRQASLWWEQHGAELSALPKLRVVEIDGASVRSLAAMAAKSLELSCLVQDGQ
                     AWIDDGDTRLELELRALKERSDR"
     gene            1821..4823
                     /gene="purL"
                     /locus_tag="PLQ29_05055"
     CDS             1821..4823
                     /gene="purL"
                     /locus_tag="PLQ29_05055"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HPG86048.1"
                     /translation="MEVTTDVYRFIVRPKERPGGRRRHEACPLAADARALGLSSVRAI
                     ERAALYFVRGTMAPKELCLLGRFLFSDPLVEDCSWSRVGDEEPDEAAAPGDDGGDDGG
                     DDGDAMRRVEAHLVETALRPGVTDPVADEAMRAAAELGVAGVEAVSSGEAFFIHGDGL
                     SDEELSLLAERLLCNAVIQRWAVGAIEPAWPDEAAGSGAVERFELAAMDDEALLALSA
                     ERRAALDLAEMAAIRGYYRREGRACTDAEFETIAQTWSEHCVHKTFKARIEVRADGEA
                     AAPYPPVVDNLLKSYIKRATDEIAAPWVLSAFVDNAGVIEFDGEHELSFKVETHNHPS
                     AVEPFGGANTGVGGVIRDVMGVSARPIAATDVLCFGPPDASPEDLPVASLHPRRVASG
                     VIAGVQDYGNKMGVPTVNGGIHYDPGYAANPLVYCGCAGIAPRGRHPRSQAAGDRVVV
                     LGGRTGRDGLRGATFSSMVMDSSTGELSGASVQIGAPIVQKKVSEALVLARDAGLYTA
                     VTDCGAGGLSSAVGEMAAELGADVDLAHAPLKYPGLAPWEIWLSEAQERMVVAVPPDR
                     LDELRSICSGLDVELTDLGRFSGDRRLVVRYDGAVVIDLDCGFLHAGPPQRSLVASPP
                     KDGPADGERKAAEPTASVARATAPSSGEALLAILAHPAVRSREATVRLYDHEVQGATM
                     LGPYAGLKADGPQDAAVLRPRESRGQVAVSLSNGFNHRYGAADPYRMAVSALDEAVRN
                     AVAVGADPDRIAVLDNFCLGDPTVPETMWTLLEAARGCYDAATAHRTPFISGKDSFNN
                     AYLAPDGRRVSVPPSLLISAIGIVPDAGAVPGSDLKAPGDRLYLVGTMEPSFGGSVYA
                     DLYGVPDGAAPGVPAASPGAPMAYRALHRAIAAGLVRACHDLSDGGLATALAEMCLGG
                     RLGAKATAPGDGLVAAALFGETNGCLVVEVADTAAAEFERIMAGIECRRIGETDGGGR
                     LELVAGDEGFAAALDDLAVAFGGTRSER"
BASE COUNT          791 a         1714 c         1667 g          760 t
ORIGIN      
        1 cccggatcat cgggataacg taggccttcg taacgcaatg agaacgagga cgccgctgat
       61 ctatttcagg gaagtgcaga gccatcgcta tcaggcgatc tggcccgtca tcgtcctcga
      121 agacgtccag gagcgtttgt gcgtacgcgc cgcgatagat cccgcgtacg ccgaactacg
      181 tcccggccta tcattctccg atatcgccga atcgccgctg gacctacggc gctacgcctg
      241 gacccagacg aggcataggc tccatcaagg cgcgttccgc gagctggtcg tttctgccta
      301 cgaagaacgc tgcgccattt gccggctgaa ccatcccgag ctgctcgatg cggcgcatat
      361 catccccgat tcggacgagc gaggcacgcc gatcgtggcc aacgggctgt cgctgtgcaa
      421 gatacaccat gccgcctatg atcaaggcat cctcggcatc gacgccgatt atcgcgtcca
      481 tatcagggag gatattctcc tagaacgcga cggcccgatg ctcaagcacg gcatccagga
      541 gctcgacggt tccacgctca tagtacctcg gcacgcggat gagcgccccg accgagaccg
      601 gctggccgaa cgatactcga ggttcttcgt cgcttaaggt cgttcgtgcc tacagcccta
      661 tcatcttctt cgcgacgggt acgtcggctg gcgcccagtc gagggttccg agctcgtcgg
      721 gacgtagcca tcgtattgcc gaatgttcgc gcagctcagg cctcccctcg agaatggtag
      781 cccggtacgc gtgcatggtg acggcgaagg cggggtacca gtgccgaacg gtcagaaaca
      841 acggccccgt caggatacgg aggccgagtt cctcgtcgat ctcgcgctcg agcgcccgct
      901 cgcgcgtctc gcccggctct accttgccgc cggggaattc ccatttcagc gacagctccc
      961 cgccggcctt tcgctgagcg cataggatcc tgccgtccag ctcgatgacg gccgctacta
     1021 ctatgagtcg cggaagggct ccgacggcgt tacataccat ggtcgccagc gtacacccgg
     1081 aacgccggcg cgtccatgcg gcctcggatt gcgctgccca gccatcctcg ccatactcct
     1141 taccgacccg gggaggatag tccatggcca tcggagcgac agtctgcaag gttactttga
     1201 gtgtatcgga cctagaccgg cattactacg ccgagcatag gttgaccgta tcgcgcgagc
     1261 cgtcggagcc ggacgagcgc ttcatggcgc gcgtcctggc cttcgcgctg aacgccgacg
     1321 atggtctggc cttcgccagg ggacaggcgg aggccgacga gccggagctg tggcgccgcg
     1381 gcctcgacgg cgggataggc ctctggatag agctcggcct gcccgatccc aagcgcctgc
     1441 gcaaggcctc ggcccgggcg gacgaggtgc tcctctacct gtaccacggc cgccaggcct
     1501 cgctctggtg ggagcagcac ggcgcggagc tgtcggcctt gccgaagcta cgcgtcgtcg
     1561 agattgacgg cgcgagcgtc cgtagcctgg ccgcgatggc cgccaagtcg ctcgagctgt
     1621 cctgcctcgt ccaggacggc caggcctgga tcgacgacgg cgatacgcgg ctcgagctgg
     1681 agctgcgcgc cctcaaggag cgctccgacc gctgatcggg cagcttaccg ccgccgcctc
     1741 gtcgctcggc cgatcgctag acgcaaacaa catcctgcgg ggtacttgac gacgctacgc
     1801 gataggcagt agtaatcctc atggaagtca cgacggacgt ttaccggttt atcgtcaggc
     1861 ccaaggaacg gccaggcggg agacgcaggc acgaggcctg cccgctcgcc gcggacgcgc
     1921 gcgcgctcgg cttgtcgagc gtccgcgcga tagaacgcgc ggccctatac ttcgtgcgcg
     1981 gcacgatggc gcccaaggag ctctgcctcc ttgggcgctt tctttttagc gaccccctcg
     2041 tcgaggactg ctcatggtcg cgtgtcggag acgaggagcc cgatgaggcg gcggccccgg
     2101 gggacgatgg aggagacgat ggaggagacg acggggacgc gatgaggcgc gtcgaggccc
     2161 atctcgtaga gacggcgctc cggccgggcg tgaccgaccc ggtcgccgac gaggcgatgc
     2221 gagcggccgc cgagctcggc gtggccggcg tcgaggccgt atcgtcgggc gaggcatttt
     2281 ttatacacgg cgacggcctg agcgacgagg agctgtccct gctcgccgag cgcctcctgt
     2341 gcaacgcggt gatccagcgc tgggccgtcg gcgcgatcga accggcctgg cccgacgagg
     2401 cggccggctc cggcgcggtg gagcgcttcg agctggccgc gatggacgac gaagcgctgc
     2461 tcgcgctgtc cgccgagcgc cgggcagccc tcgacctggc cgagatggcg gctatacgcg
     2521 gctactaccg ccgcgagggc cgcgcctgca ccgacgccga gttcgagacg atagcgcaga
     2581 cctggagcga gcactgcgtg cacaagacct tcaaggcccg aatcgaggta cgggccgacg
     2641 gcgaggcggc ggcgccctac ccgcccgtcg tcgacaacct actcaagagc tacatcaaac
     2701 gggccaccga cgagatcgcc gcgccctggg tcctatcggc cttcgtcgac aacgccggcg
     2761 tcatcgagtt cgacggcgag cacgagctgt cgttcaaggt ggagacgcac aaccacccgt
     2821 ccgcggtcga gccgttcggc ggcgccaaca ccggcgtcgg cggcgtcata cgcgacgtga
     2881 tgggcgtgtc ggccaggccg atagcagcta ccgacgtgct gtgcttcggc ccgccggacg
     2941 cgtcgccgga ggacctgccg gtagccagcc tgcatccgcg gcgcgtcgcc tccggcgtga
     3001 tagccggcgt ccaggactac ggcaacaaga tgggcgtacc gacggtcaac ggtggcatcc
     3061 actacgaccc cggctatgcg gccaacccgc tcgtatactg cggttgcgcc ggcatcgcgc
     3121 cgcgcggcag acatccgcgc tcgcaggcgg ccggcgatcg cgtggtcgta ctgggcggcc
     3181 gcaccggccg cgacggccta cgcggcgcga ccttctcgtc gatggtgatg gactcgagca
     3241 ccggagagct atcgggggcg tcggtgcaga tcggcgcgcc tatcgtacag aagaaggtat
     3301 ccgaggccct cgtcctggcg cgcgacgccg ggctctatac ggccgttacc gactgcggcg
     3361 ccggaggcct ctcgtcggcg gtcggcgaga tggcggccga gctcggcgcc gacgtcgacc
     3421 tggcccatgc gccgctcaag taccctggcc tagcgccatg ggagatctgg ctatccgagg
     3481 cccaggagcg tatggtcgtc gcggtaccgc ccgacaggct cgacgagctg cgctcgatct
     3541 gctccggcct cgacgtcgag ctgaccgacc tcggccggtt ctcgggcgac aggcgcctcg
     3601 tcgtgcgcta cgacggcgcc gtcgtcatag acctcgactg cggcttcctg cacgcggggc
     3661 cgccgcagcg ctccctcgtc gcctcgccgc cgaaagatgg gccggccgac ggcgaacgca
     3721 aggcggccga gccgacggcg agcgtcgctc gggctacggc tccatcgagc ggtgaggccc
     3781 tgctcgccat cctggcccat ccggcggttc gttccagaga agccaccgtg aggctctacg
     3841 accacgaggt gcaaggcgcc accatgctcg gcccctacgc gggcctcaag gccgacggcc
     3901 cgcaggacgc cgccgtgctc cggccgcgcg agtcgagggg tcaggtggcg gtctccctgt
     3961 cgaacggctt caaccatcgc tacggcgcgg ccgaccccta tcgcatggcg gtctccgccc
     4021 tcgacgaggc cgtacgcaac gcggtggccg tcggcgccga ccccgaccgg atcgcggtgc
     4081 tcgataactt ctgcctcgga gacccgacgg tacccgagac gatgtggacg ctcctcgagg
     4141 cggcccgcgg ttgctacgac gcggcgacgg cgcaccggac gcccttcata tccggcaagg
     4201 actccttcaa taacgcctac ctggctccgg acggccgcag ggtatcggtg cctccctcgc
     4261 tcctgatctc ggctatcggc atcgtgcctg acgccggggc cgtgccaggt tcggacctca
     4321 aggcgccggg cgacaggctg tacctcgtag gcaccatgga gccgtcgttc ggcggctcgg
     4381 tctacgccga cctgtacggc gtccccgacg gcgccgcccc gggcgtaccc gccgcctcgc
     4441 caggggcgcc catggcctat cgcgccctcc accgggccat cgccgccggc ctcgtacgcg
     4501 cctgccacga tctatccgac ggcggcctgg cgacggctct cgccgagatg tgcctcggag
     4561 ggcggctcgg cgccaaggct accgcgcccg gcgacggctt ggtcgccgcg gcgttgttcg
     4621 gcgagacgaa cggctgcctc gtcgtcgagg tcgcggatac ggccgccgcc gagttcgaac
     4681 gcatcatggc cggtatcgaa tgccgcagga taggcgagac cgacggtgga ggccgcctcg
     4741 aactggtcgc gggcgacgaa ggcttcgccg ccgcgctcga cgacctggcc gtggccttcg
     4801 gcggaacgag gagcgaacga tgagacgacc caaggcgata gtgctacacg ccgcaggaac
     4861 gaacagggac cccgacgcgt gcgtcgcgct ggagctggca ggcgccgatc cggaggtcgt
     4921 acacgttaac cg
//
LOCUS       DAOPHV010000142         4928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_22913_length_4928_cov_5.249538, whole genome shotgun sequence.
ACCESSION   DAOPHV010000142 DAOPHV010000000
VERSION     DAOPHV010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22913_length_4928_cov_5.249538"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2932
                     /locus_tag="PLQ29_05060"
     CDS             <1..2932
                     /locus_tag="PLQ29_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86049.1"
                     /translation="LSYSMTVTDYAGNSVTRNSGATGSVTVDRTPPTVHVASAGNKTI
                     GRYGETISFTATFVDTNGIDETTAPRIIIGGSSHTMTKSTNLVWTYDYPVPTGDSAPS
                     FAVTIDATDVAGNDNAVETGVQSVIVDTTRPTTSLTSPALPAYPSSAATSITAASSAD
                     VSATYYTTGTSITFAWAGADTSSGIAGYYVDGTFTADTDGSASVPLAASATAHVVYVV
                     DNAGNESYRLNITVTQDTHAPELTSIGAPSGAGIHFNGSSTYFTTAATLSFNPTATDT
                     DTGDSGLKGFNLDGTTTTVALPLSLSAGASTHTIYAVDHVGRITPSAAITVTRDQVRP
                     TTSLTSPATGVYPASAATSITATSEANVSATYYTTGDSVTFAWAGADTSSGIAGYYVD
                     GTFTADTDGSASIELAASATAHVVYAVDRVGNESYRMNVTVTKDTTRPTTSLTSPALP
                     AYPSSAATSITAASSADVSATYYTTGTSITFAWAGADTSSGIAGYYVDGTFTADTDGS
                     ASVPLAASATAHVVYVVDNAGNESYRLNITVTQDTDKPTIGSDSLVDADYPTVALRTN
                     GTMTLTFTPTDTGSGISRYTVEGACSSVAVTSGLTSGSPVERGLSGITASGDIVLTVY
                     DGVGNNETKTYTVTFDNSPPANVTGFTATANAGSSITLKWTDPTASDFDHVHISWTIN
                     GSAGSSADIEAEEEEYTISDLTENDVVVFTVSTVDTIGNASSGDVMGGSTTVLASRYS
                     SQTSSRASPTASTASTASTAFTASTASTAAAAAATTSARPGSRAITSPKATSPETKSA
                     SWVTPPTPAGRARSEAVSATTGAARADASTAARAAASSRSEAAEAKAPTTAATASDEP
                     DATPPATVVRHSTAQSVSAAGGSGAAATPSSAASSVAFAGSTVGGPATAAFNGSAQAV
                     ADGPGGSNAEAAPTTSGLGMPSDTPDAPRAPDGTGSRRPVQAVMPGRGGCGRRDEEME
                     GDER"
     gene            3050..3847
                     /locus_tag="PLQ29_05065"
     CDS             3050..3847
                     /locus_tag="PLQ29_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86050.1"
                     /translation="MSQKRVCIAAALALAATIGAFAAPVVVAFVDGDAQALAGGSWKR
                     LDFDDKLDSAQSVKLAAGAVLELRSQAGSTIAIAAPGTYVIDALFKPKAEASALSTVA
                     GKLEKLAKGGPADQTVAGVRGSEAVVAKGTMWAGGTVEADEAAKAAFEAASVGDHAKA
                     YSLYMDAFQLYVDAFDPTGAARVAYSASLSAIAFGSGARALAALRSASPEDAGALRSS
                     YALALAALSARYGAAPDAKALLKKAIDGGWFDDPASLADAKSLLAGL"
     gene            3969..4790
                     /locus_tag="PLQ29_05070"
     CDS             3969..4790
                     /locus_tag="PLQ29_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPG86051.1"
                     /translation="MDHSDDTPKPIDAHFHADDLYAVAPGFVDEYRELGVLGLASVHD
                     DAGFEATRRIMAGSRAAPVSFGLHPQLPVMDGADALAARAAAGDIAAVGECGFDFYGD
                     SPATTRTPESEAVQRAVFEYQLELAEQRGLPIVLHLRRADDLLFEYAGRLARLRAVIL
                     HSWPGPANEALDFLKRCPSALFSFGLPLCNGNKKAAASAAALPASALLTETDAPYQPP
                     RSPASEGGRPLRAWSTTGDLAGVVARLATLRGKDERSVAAAVEANFREAFGHALR"
BASE COUNT          837 a         1839 c         1522 g          730 t
ORIGIN      
        1 gctcagctac tcgatgacag tcaccgacta cgccggcaac agcgtgacga ggaactccgg
       61 cgctaccggg tccgttaccg tcgaccgaac gccgccgacc gtccacgtgg cgagcgccgg
      121 gaacaagacc atcggccgat acggcgagac catttcgttc accgccacct tcgtcgatac
      181 caacggcatc gacgagacca cggcgccgcg aataatcatt ggcggttcgt ctcatacgat
      241 gaccaagtcg acgaacctcg tttggaccta tgattacccg gtgccgacgg gcgactcggc
      301 tccaagcttc gcggtaacca tcgacgccac ggacgtggcg ggcaacgata acgccgtcga
      361 gacaggcgtc cagagcgtaa tcgtggatac cacacggccg accacctcgc tgacgtcccc
      421 ggcgctaccg gcgtacccgt cgagcgccgc cacctcgatc acggcggcct cctcagccga
      481 cgtctcggcg acctactaca ccaccggcac gagcataacc ttcgcctggg ccggcgccga
      541 cacgtcctcg ggcatagccg gctactacgt cgacggaacc ttcaccgccg acaccgatgg
      601 ctccgcgagc gtgccgctcg ccgcgtccgc gacggcgcac gtcgtctacg tcgtcgataa
      661 cgccggcaac gagtcatacc gcctgaacat caccgtaacc caggataccc acgcgcctga
      721 gctgacctcg atcggcgccc cgagcggggc gggcatccac ttcaacggct cgtcgaccta
      781 cttcaccacc gccgccactc tctcgttcaa cccgaccgcc accgataccg ataccggcga
      841 ctccgggctg aagggcttca acctcgacgg cacgaccacg accgttgcct taccgctctc
      901 cctctcagcc ggggcttcga cccataccat ctacgcggtg gaccacgtcg ggcgcatcac
      961 cccaagcgca gccatcacgg tcaccaggga ccaggtcagg ccgaccacct cgctgacctc
     1021 cccggccacc ggcgtctacc ccgcgagcgc cgccacttcg atcacggcta cctccgaggc
     1081 caacgtctcg gcgacctact acaccacggg cgacagcgtc accttcgcct gggccggcgc
     1141 cgacacatcc tcgggcatag ccggctacta cgtcgacggc accttcaccg ccgacaccga
     1201 cggctccgcg agcatcgagc tcgcagcgtc cgccacggcg cacgtcgtct acgccgtcga
     1261 cagggtcggc aacgagtcgt accgcatgaa cgtcaccgtc acgaaggata ccacacggcc
     1321 gaccacctcg ctgacgtccc cggcgctacc ggcgtacccg tcgagcgccg ccacctcgat
     1381 cacggcggcc tcctcagccg acgtctcggc gacctactac accaccggca cgagcataac
     1441 cttcgcctgg gccggcgccg acacgtcctc gggcatagcc ggctactacg tcgacggaac
     1501 cttcaccgcc gacaccgatg gctccgcgag cgtgccgctc gccgcgtccg cgacggcgca
     1561 cgtcgtctac gtcgtcgata acgccggcaa cgagtcatac cgcctgaaca tcaccgtaac
     1621 ccaggataca gacaaaccga ccatcggttc cgactccctc gtcgacgccg actacccaac
     1681 ggtagcgcta cggacgaacg gaaccatgac acttaccttc acaccgaccg ataccggtag
     1741 cggtatctcg cgctacacag tcgaaggtgc gtgtagtagc gtagcagtca cgagcggcct
     1801 cacgagcggc tcgccagtag agagaggtct ttcaggtata accgcatccg gggatatagt
     1861 gctcacggtg tacgacggcg tcggcaataa cgagacgaag acttacacgg ttaccttcga
     1921 caatagcccg ccggctaacg ttaccggatt cactgctacc gccaacgccg gttcaagcat
     1981 tacgctgaag tggaccgacc cgaccgcctc cgacttcgat catgtacaca tttcgtggac
     2041 gatcaatgga tcggccggat cgagcgccga tatcgaggca gaagaagaag agtatacgat
     2101 aagcgatctt actgaaaatg atgtcgttgt ctttaccgta tcgaccgtag ataccatagg
     2161 caacgccagc tccggcgacg ttatgggagg gagcacgacg gtcctcgcgt ctcgctactc
     2221 atcccaaacc tcatctcgag cttcgcctac cgcctccacc gcctccaccg cctccaccgc
     2281 cttcaccgcc tccaccgcct ccaccgcggc cgccgcggcc gccacgacct ccgcccgccc
     2341 gggctcgcgg gcgattacat cgccgaaggc gacctccccc gagaccaagt ccgcctcctg
     2401 ggtaacgcct ccgacgcccg ccggccgggc caggtccgaa gcggtgagcg cgacgacggg
     2461 agcagccagg gcggacgcct cgacggccgc gcgcgccgcc gcgtcgagcc gctccgaggc
     2521 cgcggaagcg aaggctccga cgaccgccgc gacggcgtcc gacgagcccg atgctacgcc
     2581 gccggcgacc gtggtaaggc actcgactgc ccagtccgtt agcgcggccg gcggttcagg
     2641 cgccgcggcg acgccctcca gcgccgcttc gagcgtggcc ttcgccggct cgacggtcgg
     2701 cggtcccgct acggcagcct ttaacggttc cgctcaggcg gtggccgacg gccccggcgg
     2761 ctcgaatgcc gaggccgcgc ctacgacaag cggcctgggc atgccatccg acaccccgga
     2821 cgcgcctcgg gcgcccgatg gaacgggctc ccgccggccg gttcaggccg tgatgccagg
     2881 cagaggcggc tgcggacgcc gcgacgaaga gatggaagga gacgagcgat agtatcgtaa
     2941 gtcttttcca gttagggttt tgaaacggtg tccgatccgc gctatactat gcgggtcgac
     3001 cgaataatcg accggggcgg ccatgcagcg cctccgaaag gtgaagcgta tgagtcagaa
     3061 acgtgtatgc atagcggcgg ccttagcgct cgccgccacg atcggcgcct tcgccgcgcc
     3121 ggtcgtggtc gctttcgtcg acggcgacgc ccaggctctc gccggaggct cgtggaagag
     3181 gctcgacttc gacgacaagc tcgactcggc ccagtcggtc aaattggcgg ccggcgcggt
     3241 actcgagctg cgctcgcagg ccggctcgac catcgccata gccgcgccgg gcacctatgt
     3301 gatcgacgcc ctgttcaagc ccaaggccga agcgagcgcc ctttcgacgg tggcgggcaa
     3361 gctcgagaag ctggccaagg gcggcccggc cgaccagaca gtggcgggcg tacgcggttc
     3421 cgaggccgtc gtcgccaagg gtaccatgtg ggccggcggc acggtcgagg ccgacgaggc
     3481 cgccaaggcc gccttcgagg cggcgagcgt cggcgatcac gccaaggcct actcgctata
     3541 catggatgcc ttccagctct acgtcgacgc attcgacccg accggcgcgg cccgcgtggc
     3601 ctacagcgcc tcgctatccg ctatcgcctt cggctcgggc gcccgcgcgc tggccgcgct
     3661 caggtccgcc tcgcccgagg acgccggagc cctgcgcagc tcgtacgcgc tggccttggc
     3721 cgcgctctcc gcccgctacg gagccgcccc cgatgccaag gccctgctca agaaggccat
     3781 agacggcggc tggttcgacg accccgcctc gctggccgac gccaagagcc tactcgccgg
     3841 gctctagcgt tcgagcctcc gcctcggaac gaaggccgcc tccagcccgg cggcggcctt
     3901 tcctttccgc ccctcgatgc tcgcggccgt cttcgcgtgc cgcccgatag tcgttatact
     3961 catcgtcgat ggaccatagc gacgatacgc ccaaaccgat agacgcccac ttccacgcgg
     4021 acgacctgta cgcggtcgcc cccggcttcg tcgacgaata ccgggagctc ggcgtcctcg
     4081 gcctcgcgtc ggtgcacgac gacgccggct tcgaggcgac tcggcggatc atggccgggt
     4141 cgcgcgccgc gccggtgtcg ttcgggctgc acccgcagct accggtgatg gacggggcgg
     4201 acgcgctcgc cgcccgggcg gcggctggcg atatcgcggc cgtaggcgag tgcggtttcg
     4261 acttctacgg cgactccccg gcgacgacga ggacgcccga gtcagaggcc gttcagcggg
     4321 cggtcttcga gtaccagctc gagctggccg agcagcgcgg cctgcctatc gtgctgcacc
     4381 tgcgccgagc ggacgacctg ctcttcgagt acgccgggcg cctggcccgg ctacgcgcgg
     4441 tgatcctgca ttcctggccg ggacctgcca acgaggctct cgatttcctc aagcgctgcc
     4501 cgagcgcgct gttctcgttc ggcctccccc tgtgcaacgg caacaaaaag gctgccgcga
     4561 gcgccgcggc cctgccggcc tcggcgttgc tgaccgagac ggacgccccc taccagccgc
     4621 ctcgctcgcc ggcttcggag ggcggacggc ccctgcgcgc ctggtcgacg acaggcgacc
     4681 tcgccggcgt ggtcgcccgt ctggccacgc tacgcggaaa ggacgagcgg tccgtggcgg
     4741 ccgccgtcga ggctaacttc agggaggcgt tcggccatgc ccttcgatga gcgcacgagg
     4801 atactgctcg gcgacgaggg ggccgacgcg ctggccaccg cgcgggcctg cgtgttcggc
     4861 ctcggtggcg tcggcgccgc cgcggcgatg gacctggtcc gcgccggcgt cggctcgctc
     4921 gtcggcgt
//
LOCUS       DAOPHV010000143         4857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_23357_length_4857_cov_4.539359, whole genome shotgun sequence.
ACCESSION   DAOPHV010000143 DAOPHV010000000
VERSION     DAOPHV010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23357_length_4857_cov_4.539359"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="PLQ29_05075"
     CDS             complement(<1..1218)
                     /locus_tag="PLQ29_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPG86052.1"
                     /translation="MKRMIAAILLIAVAAVGGFAQEKVTLTVMNYQQADQAGYPEDVK
                     IWQDFQAANPDITLNIEVLFNDPYHQKLQAYAAAGTMPDVFYVWPGARSAVIHEKKLA
                     KDLNQLLGAAYLKDFSASATNPANQLGGYMAMLPQSFTYTSVMYVNTKLLADNGFKPP
                     KTYADLKKMVPKLKAKGIAVAMLPNKDAWPMQSCLFSTVVGRMAGDDFLDAILAGKAK
                     FTDKPFVAALEVIQNLYKDGILSREDVQIGYGEAAGMFASGKAAIYIDGDWRAGAYIT
                     DKASGKALISPAAQDKDFALMPFPAIPGEKNRGVVSAVAGTGWAVSASLPAGSAKEKA
                     ATRLIQYLYSNDVQAIRFNTGAYVPTRKGVKADVEPLVAKVPVFYAENPATCYVIDGV
                     LDPAVYNVINDGLI"
     gene            complement(1289..2422)
                     /locus_tag="PLQ29_05080"
     CDS             complement(1289..2422)
                     /locus_tag="PLQ29_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HPG86053.1"
                     /translation="MATGTVPRTAARVFRSVWRKPGISRIELAGALGLDKSTVTNQVN
                     HLIDLGLILETEEGESSVKGGRRPIQLVINRKFGRVIGIEVQLGMYVAVVVDLSGEIL
                     GERRGRLDITEGNFAENVLRIVAETTAEFGCADDLLGVGVGIGGLVDSKKGRIRYSVP
                     IGITRPIDFGKAVASRLTVPCSVENDANCCAWGELAFNRGDEPRDFLFALVEYRRPLA
                     LSLRGGVGVGFGIVLGGKVHAGVHGNAGEFRSVFCDGPGELQFSLREDELSRLDTDVD
                     ALGRTADELARNMAMLVNTMDFDRVYIGGDIEALNVDLPGALRLRLEENWMYPAPKDV
                     DIRYSSLGGKAVAYGAAGMIFDRLVTERLLPGLGADPPSGHTM"
     gene            2552..>4857
                     /locus_tag="PLQ29_05085"
     CDS             2552..>4857
                     /locus_tag="PLQ29_05085"
                     /inference="COORDINATES: protein motif:HMM:NF036809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HPG86054.1"
                     /translation="MVRIDLAGTWRLERLSDHSVRPMAAPGDIMSALVASGEIPDPYY
                     DREELGLQWIGREDWRLYRSFDATERLLKRRRVRLEIDSVDTIAEISLNGRIVGASDN
                     MFLRFSADVTELLRPGENELSVTLRSPERAAADRAGRLRYPVPASVYPVSSPHRNLVR
                     KAQCMGGWDWGPCLMTGGLYDGIRLVGADGPMIDYVRYAATPEAGSGRDGAWTVELAI
                     DIDSPEAAEASLSLSLAGASLEKTVAVDAGRSVLRERLRVESPERWWPAGYGDQRLYE
                     LAAEIGPAGSTGPAATTDPDASYNASIRVGFRELHVDTTEDAHGKPMTFVVNGRPVFA
                     KGANWIPADALPSRWTRARYESLLRSAAEANMNCVRVWGGGRYESDAFYELCDELGLL
                     VWQDCMFSCALYPSSKEFLSSVEAETRHQVRRLASHPSLALWCGNNEALGAITWYDES
                     RNSPARYIVDYDRLTEGTVGRVIRELDPGRTWWPSSPSAGPDDFADNWHSDSRGDMHY
                     WSVWHEGRPFSDYLSVRPRFCSEFGFQSFPSYRTVASFAPDGERNVSSPAMEHHQRHP
                     RGNALIVETMLRYFRMPRGLRETLYLSQVQQAMAIKTAVEYWRCNRPRCMGALYWQLN
                     DVWPGASWSSLEYDGSWKLLHHEAKRFFDPLIAAAFVRDGSVQVYAVNDGAEALEAEA
                     RLTLRRFDGSAAAELATFDAVLGAEAATPLWSAPVAGLPTSPGEAFLDVELSARRAGR
                     VETRRTALFLTEPKACAVRDAGIRTNVDA"
BASE COUNT          803 a         1635 c         1589 g          830 t
ORIGIN      
        1 gatgaggcca tcgttgatca cgttatacac ggcggggtca aggacgccgt cgatcacgta
       61 gcaggtagcc gggttctcgg cgtagaagac cggaaccttg gcgacgagcg gctcgacgtc
      121 ggccttgacg cccttgcggg tcgggacgta ggcgccggtg ttgaagcgga tagcctggac
      181 gtcgttcgag tacaggtact ggatcagcct ggtcgcagcc ttctccttgg cggagccggc
      241 ggggagcgac gcggaaacgg cccagccggt gccggcgacc gcggagacga cgccgcggtt
      301 cttctcgccg gggatagccg ggaacggcat cagcgcgaag tccttatcct gggcggccgg
      361 ggagatcagg gccttgccgg aagccttatc ggtgatgtag gcgccggccc tccagtcgcc
      421 gtcgatgtag atggcggcct tgccggacgc gaacataccg gcggcctcgc cgtagccgat
      481 ctgcacgtcc tcgcgggaga ggataccgtc cttatacagg ttctggatca cctcgagggc
      541 cgcgacgaac ggcttatcgg tgaacttcgc cttaccggcc aggatcgcgt cgaggaaatc
      601 gtcgcctgcc atgcgtccga ccacggtgga gaacaggcag gactgcatcg gccaggcgtc
      661 cttgttcggg agcatggcga cggcgatgcc cttggccttg agcttgggca ccatcttctt
      721 gaggtcggcg taggtcttgg gcggcttgaa gccgttatcg gccaggagct tcgtgttcac
      781 gtacatcacc gaggtgtagg tgaaggactg gggcagcatg gccatgtagc cgccgagctg
      841 gttggccgga ttggtggcgg acgccgagaa atccttgaga taggcggctc cgaggagctg
      901 gttcaggtcc ttggccagct tcttctcgtg gatcaccgcg gaacgtgcgc ccggccagac
      961 gtagaacacg tcgggcatgg tgccggctgc cgcgtaggcc tggagcttct ggtggtaggg
     1021 gtcgttgaac agcacctcga tattgagggt gatgtcggga ttggcggcct ggaaatcctg
     1081 ccagatcttg acgtcctccg ggtagccggc ctggtcggcc tgctggtagt tcatgacggt
     1141 cagcgtcacc ttctcctggg cgaaaccgcc gacggccgcg accgcgatca ataggatcgc
     1201 cgcgatcatt ctcttcattc tagcctcctt gataacgggc ccgcggcccg cctcagaaac
     1261 cgttctatat cgacccctac ggggacgttc acatggtatg cccgctcggc ggatcggcgc
     1321 cgagccccgg gagcagccgc tccgttacca gtcggtcgaa gatcatgccg gccgcgccgt
     1381 acgcgacggc cttaccgccg agactcgagt agcggatgtc cacgtccttc ggggccggat
     1441 acatccagtt ctcctcgagc ctgagcctga gcgcgccggg cagatccacg ttcagagcct
     1501 cgatgtcgcc gcctatgtag acgcggtcga agtccatcgt gttgacgagc atcgccatgt
     1561 tcctggccag ctcgtcggcg gtcctcccga gggcgtcgac gtcggtatcg agcctggaca
     1621 gctcgtcctc gcggagcgag aactgcagct cgcccggacc gtcgcagaac acgctgcgga
     1681 actcgccggc gttaccgtgc acgcccgcgt gcaccttgcc gccgagcacg atgccgaagc
     1741 ccacgccgac gccgcctcgc aggctcaggg ccagaggcct ccgatactcg acgagcgcga
     1801 acaggaaatc gcgcggctcg tcgccgcggt tgaacgccag ctcgccccag gcgcagcagt
     1861 tcgcgtcgtt ctcgacggaa cacggcaccg tgagcctcga ggcgaccgcc ttgccgaaat
     1921 ctatgggcct ggtgatgcca atcggaacgg aatagcggat tcggcccttc ttcgagtcga
     1981 ccaggccgcc gatgcctacg ccgacgccga gcaggtcgtc cgcgcagccg aattcggcgg
     2041 ttgtctcggc tacgatgcgc aacacgttct cggcgaaatt gccctccgtg atgtcgaggc
     2101 ggcctcgcct ttccccgagg atctcgcccg acaggtcgac cacgacggcc acgtacatgc
     2161 cgagctgaac ctctatgccg atcacgcggc cgaacttccg gttgatcacg agctggatgg
     2221 gccgacgacc gcccttgacc gacgactcgc cctcctcggt ctcgaggatc aggccgaggt
     2281 ctatgaggtg gttgacctgg ttggtgacgg tggatttatc gaggcccagc gcgccggcca
     2341 gctcgatacg gctgatgcct ggcttacgcc atacggacct gaaaaccctc gcggccgtcc
     2401 tcggaaccgt tccagtcgcc accgctcctc ctccagcgcc tcacaatata agttggttga
     2461 tctaaccaac caatcatgct ataccatgag ttcagataac cgtcaacatc gtttttattg
     2521 catcgactaa aaaccctgaa cggaggagac gatggtacga atcgatctgg ccggaacctg
     2581 gaggctcgag cgcctgtccg atcacagcgt acggccgatg gcggctcccg gcgacatcat
     2641 gtcggccctc gtcgcctcgg gggagatacc cgacccgtac tacgaccgcg aggagctcgg
     2701 cctccaatgg ataggacgcg aggattggcg gctgtaccga agcttcgacg cgacggagag
     2761 gctccttaag cgacgacgcg tccgcctcga gatagacagc gtcgatacga tagccgaaat
     2821 cagcttgaac ggccgtatcg tcggcgcgag cgacaacatg ttcctacgct tctccgccga
     2881 cgtcacggag ctgctacgcc ccggcgagaa cgagctatcg gtgaccctgc gctcgcccga
     2941 gcgggcagcc gccgatcgcg ccggccgcct ccgctatccc gtaccggcct cggtctaccc
     3001 tgtcagctcg ccgcaccgca acctcgtacg caaggcgcag tgcatgggcg gttgggactg
     3061 ggggccctgc ctgatgacgg gcggcctcta cgacggcata cggctagtcg gcgctgacgg
     3121 cccgatgatc gactacgtac gctatgcggc taccccggag gcgggctccg ggcgcgacgg
     3181 cgcctggacc gtcgagctgg ccatcgatat agactcgccc gaggccgccg aggcgagcct
     3241 gagcctgtcg ctggccggcg cctcgctgga gaagaccgtc gccgtggacg ccggccgctc
     3301 cgtgctccgg gaacgactgc gggtcgagtc gccggaacgt tggtggccgg ccggctacgg
     3361 ggaccagcgc ctgtacgagc tcgctgccga gatcggaccg gccggctcga ccggcccggc
     3421 cgccacgact gaccctgacg caagctacaa cgcctcgatt cgggtcggct tccgcgagct
     3481 tcacgtcgac acgacggagg acgcgcacgg caagcctatg accttcgtcg tcaacggccg
     3541 ccccgtcttc gccaagggcg cgaactggat cccggccgac gccctgccgt cccggtggac
     3601 ccgcgcccgc tacgagtcgc tccttcgctc ggccgccgag gccaacatga actgcgtccg
     3661 ggtatggggc ggcggtcgct acgagtcgga cgcgttctac gaactgtgcg acgagctcgg
     3721 cctcctcgtc tggcaggact gcatgttctc ctgcgcgctc tatccgtcct cgaaggagtt
     3781 cctgtcctcg gtcgaggcgg aaacccgcca ccaggtcagg cgcctcgcca gccacccgtc
     3841 gctggcgctc tggtgcggca acaacgaggc gctcggcgcg atcacctggt acgacgagtc
     3901 gcggaacagc cccgccaggt atatcgtcga ctacgaccgc ctgaccgagg gcactgtcgg
     3961 ccgcgtcatc cgcgaactcg acccggggcg gacctggtgg ccgtcgtcgc cgtcggccgg
     4021 cccggacgac ttcgccgaca actggcactc cgactcccgc ggcgacatgc actactggtc
     4081 cgtctggcac gagggacgcc ccttctcgga ctacctgagc gtgcggccgc gtttctgctc
     4141 ggagttcggc tttcagtcct ttccatcgta ccgcacggtc gcctccttcg cgccggacgg
     4201 cgagcgcaac gtctcgtcgc cggcgatgga acaccaccaa cgtcaccctc gcggtaacgc
     4261 gctgatcgtc gagacgatgc tacgctactt caggatgcca cgcggcctca gggagacgct
     4321 gtacctgtcc caggtgcagc aggcgatggc catcaagacg gccgtggagt actggcgctg
     4381 taatcggccg cgctgtatgg gcgcgctata ctggcagctc aacgacgtat ggccgggcgc
     4441 cagctggtcg agcctcgagt acgacggctc atggaagctc ctgcaccacg aggccaagcg
     4501 cttcttcgac ccgctcatcg cggcggcctt cgtacgcgac ggttcggtac aggtgtacgc
     4561 cgtcaacgac ggcgcggagg cgctcgaggc cgaggcgagg ctgacactgc gccgcttcga
     4621 cggctcggcg gccgcggagt tggcaacgtt cgacgcggtc ctgggcgccg aggctgccac
     4681 gccgctatgg agcgccccgg tcgccggcct gcccacctct ccgggcgagg ccttcctcga
     4741 cgtggagctg agcgcccggc gcgctggcag ggtcgagacc cgccgtacgg ccctgttcct
     4801 gaccgagccc aaggcctgcg ccgtccggga cgcgggcatc cggaccaatg tcgacgc
//
LOCUS       DAOPHV010000144         4843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_23467_length_4843_cov_3.493525, whole genome shotgun sequence.
ACCESSION   DAOPHV010000144 DAOPHV010000000
VERSION     DAOPHV010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23467_length_4843_cov_3.493525"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PLQ29_05090"
     CDS             <1..428
                     /locus_tag="PLQ29_05090"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HPG86055.1"
                     /translation="AFNESAARLGFDGAYPLRGEAMADVPDEIAPRPGDAIIDKATYS
                     GFSTPRLEETLRGLGVSAVVMTGLATSQCVDTTARQASERGFTVVHVEDGQADYSQDE
                     HDAALFASRGVCGGHVVESEFLAAAPLRLVRAYVESEAR"
     gene            complement(444..3434)
                     /locus_tag="PLQ29_05095"
     CDS             complement(444..3434)
                     /locus_tag="PLQ29_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86056.1"
                     /translation="MSAAGLRSLTAVALFAAAALCHADPDTAASYEPAPGVLGPSPGA
                     YAATQVLVPATLSAGARAAYRFSGQEAWLPFDRPLYLSAFPGEERHYSIEFGNPRSPS
                     PTTLSYVIDLRPPEPPTFSAPPGDAGAFLSVSVASDYDVFVSVNGGPFAQAQVGSNLE
                     FRAPPDATAVVSLAAYAVDAAGNASRVASSRWRLCPEGVAPSFPFDASAPVRSVIMAD
                     AGAYLAAEVADLVGSARLTVRAPAGTVPCVAVNASETPSPASFVELSGGSSSASCVVP
                     YPWGYEIPIEIRYGYIEGGVVHMAADRLRLLPRFPAEEASMAPAEAAAPEIYAYGSAA
                     FVHWPAGPWAIMYSIADGDFIQGAAPLRVELGAQPIPIRYYALGQDGVRSAVSMATLP
                     PKASSLPPPLGGVTHGGTYGGTVIVVPGGEPRPRYELSDDGSAPRAISGSSPVLPAGG
                     LSIAGRPGETVFYRLRVAAEAESERFVSFAIDREPPSVPAIGRSLRSYAAADTLVSFR
                     EQEGAVFVSVSEDGSGPFARYDEPIAIAGSDEGRKRYVVRAYAEDEFGNRSAEMPRFE
                     LMIDRSTLYVDARGRPGASGAPDDPIAGLDDAIEEAQRAGKRFVYVRGGGSIGRSVVV
                     SGRLEVRGGFDDSWNEVPGAATAVSVRVPPSSSSYAFVVDGGALTLSSMTFSLKADGA
                     GGLALARSGSLELTRCSIESTGGIEVAAVRSTGADVTVSSSTLRSSSAVTGRGIDLSG
                     GRLVVSDSTIACDRSVRLFDAIRVSDAEAFVSGLRIDAEPSHAFSAFSASRSRASIER
                     CIVNVSGGSSSCRAFSAGSSELTASSVYVHAAWKGSVELFSAISGSTMRLAHVTAIAD
                     APAALFASATGSSVEIVNSIASFSGGGSAFLRLDGGAGSVRLASNCLWGFERLAELSS
                     HRRLIATVAELNGAFSSGAAPNRLERPSEIFQSTIKGLYRLSRSSVCVDGATAVPWAA
                     GSDLFGSPRPTGEGAAVSIGAEEL"
     gene            complement(3434..4039)
                     /locus_tag="PLQ29_05100"
     CDS             complement(3434..4039)
                     /locus_tag="PLQ29_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HPG86057.1"
                     /translation="MSKEFISYNQVRNNALKLAHRIHLDGFVPDIIYVSLRGGAYLGN
                     VMSEYFKAVRSDARPVFYAAVVARSYTDIHKNEQVRVDGWTYSPEYLRNGDKVLLVDD
                     IYDTGRTINHLASVILEKGVPRRDLKIAVHDYKIVDYRGEQPAIKPDYWCRLHEIRKP
                     EDERWIHYMSHELIGLTEEELKEYYYPEDPELQEILSVLKG"
     gene            complement(4070..>4843)
                     /locus_tag="PLQ29_05105"
     CDS             complement(4070..>4843)
                     /locus_tag="PLQ29_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPG86058.1"
                     /translation="INRGNSGGALADIDGRVIGINTWIASNTGGSIGLGFSIPINNAK
                     KVIDDIISGGSVRYGWLGVLLSDAPKATLTELALGSTKGAFIGHVFTDGPAAKGGIKP
                     GDYVVEIDGKTVSSMDQLVRIVGDLPVGSKSSFAVIRDGKRLQLRVTIDERKEASAAD
                     YSKLWPGLEIAPIDAAAREELGLDKRSGGALVTSVIAKSPASTLGLKAGDVVTEVNGV
                     AIGSVADYYRVLNDPKTQKFQFTVLRDGQTLTTLALIRK"
BASE COUNT          831 a         1564 c         1664 g          784 t
ORIGIN      
        1 gggccttcaa cgagtcggcc gcccggctgg gtttcgatgg cgcctacccg ctccgcggag
       61 aggcgatggc cgacgtaccg gacgagatcg ccccgcgccc gggcgacgcg atcatagaca
      121 aggccaccta ctccggattc tcgacgccgc ggctcgagga gacgctccga ggcctcggcg
      181 tctcggccgt cgttatgact gggctggcca cgagccagtg cgtcgatacc acggccaggc
      241 aagcgtccga gcgcggcttc accgtggtgc atgtggagga cggacaggcc gactacagcc
      301 aggacgagca tgacgcggcc ttgttcgcct cgagaggcgt ctgcggaggg catgtcgtcg
      361 agtccgagtt cctggccgcg gctccgctac ggctggtgcg agcctacgtg gagtccgagg
      421 cgcgctgaat cgccacggct cgtttatagc tcctcagccc cgatggacac ggcggccccc
      481 tcgcccgtcg gcctgggcga gccgaacagg tcgcttccgg cggcccaagg cacggccgtc
      541 gcgccgtcga cgcagaccga cgagcgggac aggcgataca gacccttgat cgtggattgg
      601 aaaatctcgg acgggcgttc gagcctgttc ggagccgcgc cagaactgaa cgcaccgttc
      661 aactcggcga cggtagcgat gaggcgacga tgggacgata gctcggcgag gcgttcgaag
      721 ccccagaggc agttcgacgc cagccggacc gacccggcgc cgccgtcgag gcgcaggaac
      781 gccgagccgc cgcccgagaa cgaggctatc gagttgacta tctcgacgct cgacccggtc
      841 gccgacgcga acagggccgc cggcgcgtcc gctatggccg tgacgtgggc cagtcgcatc
      901 gtcgacccgg atatggcgct gaatagctcg accgagcctt tccaggcggc gtggacgtag
      961 accgacgagg cggtaagctc cgaggagccg gcgctgaagg cccggcacga cgaggaaccg
     1021 cccgatacgt tgacgatgca tcgctcgatc gaggcccgcg agcgtgacgc gctgaatgcc
     1081 gagaacgcgt gcgacggttc ggcgtcgatg cgcaggccgg agacgaaggc ctcggcgtcg
     1141 ctcacgcgga tcgcgtcgaa cagccgaacc gaccggtcgc aggctatcgt cgagtccgag
     1201 acgacgagcc tgcctccgga cagatctata cccctgcccg tgaccgccga ggaggagcgt
     1261 agagtcgacg acgagacggt gacgtcggcc ccggtggacc tgacggccgc cacctcgatg
     1321 cctcctgtag actctatcga gcaacgggtc aattcgagcg agcctgagcg cgccagcgcg
     1381 agccccccgg cgccgtcggc cttgagcgag aacgtcatcg acgacagggt cagcgcccct
     1441 ccgtcgacga cgaaagcgta tgatgacgac gacggaggca ctcggaccga aacggcggtg
     1501 gccgcgcccg gcacctcgtt ccagctatcg tcgaagccac ccctgacctc gagccggccg
     1561 ctcacgacca cgctacggcc gattgaaccg cccccacgta cgtagacgaa tcgcttcccg
     1621 gctcgctgag cttcctcgat cgcgtcgtcg agcccggcta tcgggtcgtc gggcgcgccc
     1681 gaggcgccgg gtcggccgcg agcgtcgacg tacagggtcg aacggtctat catcagctcg
     1741 aatcgaggca tctccgcgga acgattaccg aactcgtcct cggcgtaggc gcgcacgaca
     1801 tagcgcttgc ggccttcgtc cgaacccgcg atggcgatcg gttcgtcgta tcgcgcgaac
     1861 ggacccgatc cgtcctcgga gaccgatacg aacaccgcgc cttcctgctc tctaaaggac
     1921 acgagcgtgt cggcggccgc gtagctacgc aggctccggc cgatggcagg gacggacggg
     1981 ggctcccggt ctatcgcgaa gctgacgaag cgttcagact cggcttcggc ggcgacgcgt
     2041 agcctataga ataccgtctc gccggggcgc cccgctatcg aaaggccgcc ggccggcagg
     2101 accgggctgg agccgcttat cgcccgtggg gcagagccgt cgtcggatag ctcgtatcgc
     2161 ggtctcggct cccctccggg gacgacaatg accgtgccgc cgtacgtgcc gccatgcgtc
     2221 acgccgccga gtggaggcgg caacgacgag gccttgggcg gtagcgtcgc catcgagacc
     2281 gccgagcgga cgccgtcctg cccgagggcg taatagcgta tcggtatggg ctgtgcgccg
     2341 agctcgacgc gcagtggcgc cgcgccttgg ataaagtcgc cgtcagcgat ggaatacatg
     2401 atggcccacg gacccgccgg ccaatggacg aaggcggccg acccgtaggc gtatatctcc
     2461 ggcgcggcgg cctcggccgg agccatcgac gcctcctcgg cggggaagcg cggtagcagg
     2521 cgtagcctat cggccgccat gtggacgacc ccgccttcga tgtagccgta gcgtatctcg
     2581 atcgggatct catagcccca ggggtacggc acgacgcagg aagcggacga cgatccgccc
     2641 gacagttcga cgaaggacgc tggcgacggc gtttccgagg cgttgacggc gacgcagggt
     2701 acggtgccgg cgggagccct gactgttagc ctggccgatc cgacgaggtc ggcgacctca
     2761 gccgccaagt aggctccggc atcggccatg atgacgctac gtaccggggc cgaggcgtcg
     2821 aacgggaacg acggcgccac gccttcgggg cacagacgcc accgagatga cgccaccctg
     2881 gacgcgttac cggcggcgtc gacggcgtag gccgcgagcg aaaccacggc ggtggcgtcg
     2941 ggtggcgcgc ggaactccag gttcgagccg acctgcgcct gagcgaaggg tccgccgttc
     3001 acgctgacga aaacatcgta atcggacgct acggataccg acaggaaagc gcctgcgtca
     3061 cccggaggcg cgctgaaggt cggcggctcg ggcgggcgca ggtctatgac atacgacaag
     3121 gtcgtggggg acggggaacg tgggttgccg aactctatcg agtagtggcg ctcctcgccc
     3181 gggaaggccg agaggtagag cggccggtcg aacggcaacc acgcctcctg accggaaaag
     3241 cggtatgcgg cgcgggcgcc ggccgatagg gtcgccggta cgaggacctg tgtagccgcg
     3301 taggcgccgg ggctcggacc gagcaccccg ggagccggct cgtaggaggc ggccgtatcg
     3361 gggtcggcgt ggcatagggc ggccgcggcg aacagcgcta cagccgttag tgatcgaagc
     3421 ccggcggccg acatcagccc ttcagtaccg agaggatctc ctggagctcc ggatcctcgg
     3481 gatagtagta ttccttgagt tcctcttcgg tcagcccgat cagctcgtgg ctcatgtagt
     3541 gtatccagcg ctcatcctcg ggcttgcgta tctcatggag ccggcaccag tagtctggct
     3601 tgatggccgg ctgctcgccg cggtagtcga ctatcttgta gtcgtgcacg gcgatcttga
     3661 ggtcgcggcg gggtacgccc ttttcgagta tgacgctcgc caggtggttg atggtccgac
     3721 cggtatcgta gatgtcgtcg acgagcagca ccttgtcgcc gttcctcagg tactcggggc
     3781 tataggtcca gccgtcgacg cggacctgct cgttcttgtg aatgtcggta taggagcggg
     3841 cgacgaccgc cgcgtagaac accggccgcg cgtcgctcct gaccgccttg aagtattcgc
     3901 tcatgacgtt gccgagatag gctccgccgc gcaaggaaac gtagatgatg tcggggacga
     3961 acccgtccag gtgtatccta tgcgccagct tgagggcgtt gttccgcacc tggttgtacg
     4021 atatgaattc cttgctcatc ggaacctctc tttgttcttt actgtagcac tacttgcgta
     4081 tcaaggcaag cgtggtcagc gtctggccat cgcgcagcac ggtgaactgg aacttctggg
     4141 tcttagggtc gttgagcacg cggtaatagt cggcgacgga gccgatcgcg actccgttca
     4201 cctcggtgac gacgtcgccg gccttgaggc cgagagtcga cgcggggctc ttggcgatga
     4261 cgctcgtgac gagcgcgccg ccggaacgct tatcgaggcc gagctcctcc ctggcggcgg
     4321 catcgatcgg cgctatctcg aggccgggcc atagcttcga gtagtcggcc gcgctcgctt
     4381 ccttgcgctc gtctatcgtc accctgagct ggagccgctt gccgtcgcgg atcaccgcga
     4441 aggacgactt ggagccgacc ggcagatcgc cgacgatgcg cacgagctga tccatcgagg
     4501 agacggtttt gccgtcgatc tcgacgacgt agtcgcccgg tttgatgccg cccttggccg
     4561 cggggccgtc agtgaacaca tggccgatga aggcgccttt cgtcgagccg agggctagct
     4621 ccgttagcgt cgccttgggc gcgtccgaca ggagtacgcc gagccagccg taacggacgg
     4681 aaccgcccga gataatatcg tctatcacct ttttggcatt attgatcggg atggagaatc
     4741 ccaagccgat gctgccgccg gtgttcgagg ctatccaggt gttgatgccg atgaccctgc
     4801 cgtcgatgtc ggccagggcg ccgcccgagt tgcctcggtt gat
//
LOCUS       DAOPHV010000145         4813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_23684_length_4813_cov_4.458386, whole genome shotgun sequence.
ACCESSION   DAOPHV010000145 DAOPHV010000000
VERSION     DAOPHV010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23684_length_4813_cov_4.458386"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PLQ29_05110"
     CDS             complement(<1..630)
                     /locus_tag="PLQ29_05110"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPG86059.1"
                     /translation="MSRAIEELEWVYGFWRGLSPYAFARTEDEVVILPPNLVYKTNRT
                     GAALLEYLGTGGRFEDLPGFDDEKAAQVGSFFLSLKAAYEGRPVELERVRYGFDFTRL
                     PVLGEIAITYRCNNRCLFCYAGCGDSSGLALAQGDGASELDTAEWIRVLDLFKDEAKI
                     PFFSFTGGEPLMRPDVEELAAYAVSRGLSVNLVTNGRLATPERAASLYAS"
     gene            complement(627..2312)
                     /locus_tag="PLQ29_05115"
     CDS             complement(627..2312)
                     /locus_tag="PLQ29_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86060.1"
                     /translation="MSSSSTRRAYGRFRWKPSLAALLLLLAALAVGSASALASDLASV
                     DITVNLRPDGRGEIFYTLEWRVSSGDMSGFYFQGEAFAPVWNLTRCYADLDGGRRVPL
                     RVDDLGSGRYDVVLEGGRRFSGTAYYVMNYAGDFAGAGLVGLTESPERGSLVYFDWAP
                     VEWDEALEYRAVRLVLPVRVSGERLGDDEKRSVPMLTESWVNEQNRIDYYGSQGDDGA
                     YYLTALFYQEAPRANAEQRLGLYFPEGYLDLDAALGSRATTDGIGRGERGDRTGGPAA
                     TIGLLAALGAAIAAALLLYRRRATKHAERASLVEAIAWAGDAWTPPKLFAGSYQVPGK
                     VPEDLHPVEAALLLELALPRIASIMVDALVAQGLVAVEREDPLKLRFLSERAAADEIE
                     ERFMASFDADGNVLPGLLADFFEGAIKSLQEKVWDCDLEATKDHYRKKLLEAGRRADE
                     VASWDESRRTAYYREHRAWMYWRSYAVISTRPDRLAGVRLPEGMTAGYADFMRSAVCY
                     SGCFTPDNVGTVGSCHSACHNACHDACHSACHSACHSACHSACHSACVSGSSH"
     gene            complement(2309..2923)
                     /locus_tag="PLQ29_05120"
     CDS             complement(2309..2923)
                     /locus_tag="PLQ29_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86061.1"
                     /translation="MDLRRYLGLYAVSPLGLAMSAASVAVGAAVGFAASPLAGVAACL
                     VTEATAFAITTASGLGPKAAVAEGERRAWAEASERLEAVKRDRDRLATMRLPDDELGS
                     LVRLAATRGASYLAASRNAKSRVPQAEEALSDCLSVADLYLKELDVASTERRHGLDDA
                     DPFADAKGRAKAALVDKAAIIEKATRDLSGGLSPADAMDIKERL"
     gene            complement(2967..3395)
                     /locus_tag="PLQ29_05125"
     CDS             complement(2967..3395)
                     /locus_tag="PLQ29_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPG86062.1"
                     /translation="MPVPGGAVTASPSPASQPGGAFELARGERGVVMLLYDLRFVADG
                     DELLASERGRLDAIAEALGRLPDRTFLVEGHTADLGKPAGQYELSERRARRIVDELAA
                     RGIPENRFVYRGLGADRPIAPNDSEANRARNRRVEITILD"
     gene            3370..>3608
                     /locus_tag="PLQ29_05130"
     CDS             3370..>3608
                     /locus_tag="PLQ29_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86063.1"
                     /translation="MTAPPGTGTPVPGADEPGADEPGADEPGEAPADGVPVAPADADE
                     PGEALAVGVPVAPADADGVPVAPADGVPVAPADADG"
     assembly_gap    3609..3708
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3709..4550)
                     /locus_tag="PLQ29_05135"
     CDS             complement(<3709..4550)
                     /locus_tag="PLQ29_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86064.1"
                     /translation="MTATRGLRCAIRVHRCVSRGLACALALSAACVAQAQAGLTPFNP
                     PEGRVIVTERSDFSRYEDGRYVGHAYREARLDLSLTRTAEGLARYSGEALVLEETMRD
                     ERAVARRLDEAVPVEFTAGPDGTLRFSVDRGYPILRGVPTSPPEDVAIGGRWTGYGSV
                     VVRPRPGHSATRVRVLVEYEYRGPTNYAGRAAEAIRARYAVRYDGRDPSGDPDMSGAT
                     GGRTADIVIDAEDGSTLFIRETIDESYTYRTYPTVRLKGFILHFHRGSLPGDRDRVVA
                     TITGA"
     gene            complement(4547..>4813)
                     /locus_tag="PLQ29_05140"
     CDS             complement(4547..>4813)
                     /locus_tag="PLQ29_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HPG86065.1"
                     /translation="GSCCYVVDEARARAFESEFGSTAVDRPDGRPRLNLVEANRALAD
                     ALGLVDPSIAGDCTCCDGRFGSFRRQGPSSFTRMAVAIGHTESV"
BASE COUNT          755 a         1624 c         1643 g          691 t
ORIGIN      
        1 ggaggcgtac agcgacgcgg cccgttccgg cgtcgcgagg cgcccgttcg tgaccaggtt
       61 gacactgagc ccgcgcgaga cggcgtaggc agccagctcc tcgacgtcag gtctcatgag
      121 aggctcgccg ccggtgaacg agaagaacgg aatcttggcc tcgtctttga acaggtcgag
      181 gacgcgtatc cactccgcgg tatcgagctc cgacgcgccg tcgccctgcg cgagggccag
      241 gccgctcgag tcgccgcaac cggcgtagca gaacaggcag cggttattac aacgataggt
      301 gatcgctatc tcgccgagca cgggtagccg cgtgaagtcg aagccgtagc gcactcgctc
      361 cagctcgacc gggcggccct cgtaggccgc cttcagggac aggaagaacg agccgacctg
      421 agccgccttc tcgtcgtcga agccgggcaa gtcctcgaag cgtccgcccg tgccgaggta
      481 ctcgagcagg gcggcgccgg tccggttggt cttatagacc aggttgggcg gcaggatgac
      541 cacctcgtcc tcggtccgcg cgaaggcgta gggcgatagc ccccgccaga agccgtagac
      601 ccactcgagt tcttcgatcg cgcggctcaa tgcgaactcc ccgaaacgca ggcggaatga
      661 caggcggagt ggcaggccga atggcaagcc gagtggcagg ccgaatggca ggcgtcgtgg
      721 caggcgttat ggcaagccga atggcacgag ccaaccgtcc cgacgttgtc gggcgtgaag
      781 cagccggagt agcagaccgc cgagcgcatg aagtccgcgt agccggccgt catgccctcg
      841 ggcaagcgca cgccggccag tcgatccggc ctcgtcgaga tgacggcgta gctacgccag
      901 tacatccagg cgcggtgctc gcgatagtag gccgtgcgac gggactcgtc ccagctcgcg
      961 acctcgtcgg cccgcctgcc ggcctcgagc agcttcttgc ggtaatgatc cttcgtcgcc
     1021 tccaggtcgc agtcccagac cttctcctgg aggctcttga tggcgccctc gaagaagtcg
     1081 gcgaggagcc ccggcaggac gttcccgtcg gcgtcgaagg aggccatgaa acgctcctcg
     1141 atctcgtcgg cggccgcgcg ctcagacagg aagcgcagct tgagcgggtc ctcccgctcg
     1201 acggccacga ggccctgggc gacgagggcg tcgaccatga tcgaggcgat gcgcggaagg
     1261 gccagctcga ggaggagcgc agcctcgacc ggatgcagat cctccggtac cttgcccggt
     1321 acctggtagc tgccggcgaa cagcttgggc ggcgtccatg catcgccggc ccaagcgatg
     1381 gcctcgacca gagaggcgcg ctcggcgtgc ttggtcgcgc gccggcgata gaggagcagg
     1441 gccgcggcga tcgccgcgcc gagggccgcg agaaggccga tggtcgcggc gggtccgccg
     1501 gtccgatcgc cccgctctcc ccggccgatg ccgtcggtcg tcgcgcgact accgagcgcc
     1561 gcgtcgaggt caaggtagcc ctcggggaaa tagaggccaa ggcgctgctc cgcgttggcc
     1621 cgaggcgcct cctgatagaa gagcgccgtc aggtagtagg ccccgtcgtc accctgggag
     1681 ccgtagtagt cgatgcggtt ctgctcgttg acccatgact cggtgagcat cggcacggag
     1741 cgcttctcgt cgtcccccaa gcgctcccca gagacgcgga cgggcagcac gagcctgacg
     1801 gcccggtatt cgagcgcctc gtcccattcg accggagccc agtcgaagta gaccagggag
     1861 ccgcgctccg ggctctcggt caggccgacg agcccggccc cggcaaagtc gccggcgtag
     1921 ttcatcacgt agtaggccgt gcccgagaac ctgcggccgc cctcgagcac gacgtcgtag
     1981 cgtcccgagc ccaggtcatc gacgcgtagc gggacgcgcc gcccgccgtc gaggtcggcg
     2041 tagcagcgcg tcaggttcca gaccggcgcg aaagcctcac cttggaaata gaagccgctc
     2101 atatcacccg acgacacgcg ccactcgagc gtatagaaga tctcgcccct gccgtcgggc
     2161 cgcaggttga ccgtaatatc gaccgacgcc aggtcgctcg ccaacgccga ggcgctaccg
     2221 acggccaggg ccgcgagtaa gaggagaagc gccgccaggc tcggcttcca gcggaagcgc
     2281 ccgtacgcgc ggcgagtact gctactgctc atagtcgctc cttaatatcc atggcgtcgg
     2341 cgggtgaaag gccgcccgac aggtcgcgcg tggccttctc gatgatggcc gccttatcga
     2401 ccagggccgc cttcgcgcgt cccttggcgt cggcgaaggg atcggcgtcg tcaaggccgt
     2461 gcctcctctc ggtcgaggcc acgtccagtt ccttgaggta caggtcggcg actgaaaggc
     2521 agtcggacaa agcctcctcg gcctggggga cgcggctctt ggcgttccgg ctggccgcca
     2581 ggtaggaggc gcccctcgtc gcggcgagtc tgacgagcga gcccagctcg tcgtcgggca
     2641 ggcgcatcgt cgcgaggcgg tcgcggtcgc gcttgacggc ctcgagccgc tcgctcgctt
     2701 cggcccaggc gcggcgctcg ccctcggcga cggcggcctt ggggccgagg ccgctcgccg
     2761 tggttatcgc gaaggcggtc gcctcggtta cgaggcaggc ggccacgcca gcgagcggcg
     2821 aggcggcgaa gccgacggcc gccccaacgg cgaccgaggc cgccgacatg gcgagaccga
     2881 gcggggagac ggcgtatagg ccaaggtatc gtcgtagatc catggcgata ctgtagcgcc
     2941 gcgccaagtc agtatcaagc ctaccctcag tcgagaatcg ttatctcgac cctgcgattg
     3001 cgcgcgcggt tggcctcgct gtcattcggc gcgatgggcc gatcggcacc gagcccgcga
     3061 tagacgaacc gattctccgg gatgccccgc gcggccagtt cgtcgacgat gcgcctggcc
     3121 ctccgctccg aaagctcgta ctggccggcc ggcttgccaa ggtcggcggt atgaccctcg
     3181 acgaggaagg tacgatcggg taggcgtccg agcgcctcgg ctatcgcgtc cagtcggccg
     3241 cgctcggagg cgagcagctc gtcgccgtcg gccacgaagc gcaggtcgta cagcaacatc
     3301 acgacgcctc gctcgccccg ggccagctcg aacgcccctc caggctggct cgctggcgac
     3361 gggctcgccg tgacggcgcc gcccggtaca ggcactccgg ttcctggcgc cgacgagcct
     3421 ggcgccgacg agcctggcgc cgacgagcct ggcgaggctc cggccgacgg cgtcccggtt
     3481 gcgccggcgg acgctgacga gcctggcgag gctctggccg tcggcgtccc ggtggcaccg
     3541 gcggacgccg acggtgtccc ggtggctccg gccgacggcg tcccggttgc gccggcggac
     3601 gctgacggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc gccggtgatc
     3721 gtcgcgacga cccgatcccg gtccccgggt aagctgccgc gatggaaatg caggatgaaa
     3781 cccttgagcc tgacggtcgg atacgttcga taggtatagc tctcatcgat cgtctcgcgg
     3841 atgaacagcg tggagccgtc ctcggcgtcg atgacgatat cggcggtacg gccgccggtg
     3901 gcgccgctca tgtccgggtc gcccgaggga tcgcgcccgt cgtatcggac ggcgtagcgc
     3961 gccctgatcg cctcggcggc gcgaccggcg tagttcgtgg ggcctcgata ctcgtactcg
     4021 acgagcaccc gcaccctcgt cgcgctatgg ccgggccgtg gcctgacgac gaccgagcca
     4081 tagccagtcc agcggccgcc tatggccacg tcctcgggtg gcgaggtcgg cactccgcgg
     4141 agtatcggat agcctcggtc gaccgagaag cgtagcgtac cgtccggccc ggcggtgaac
     4201 tcaaccggca cggcctcgtc gaggcgcctg gccaccgccc tctcatcgcg catcgtctcc
     4261 tcgagtacga gggcctcgcc cgagtagcgg gccaggccct cggcggtcct ggtcagcgac
     4321 aggtcgagcc tcgcctcccg gtaggcatgt ccgacgtagc ggccgtcctc gtagcgcgag
     4381 aagtccgatc gctcggtgac gatgactcga ccctcgggcg ggttgaaggg cgtgaggcca
     4441 gcttgagcct gcgcgacaca ggcggccgat agcgccagag cgcacgcgag accgcgcgac
     4501 acgcatctat gaacgcgtat cgcgcatctg aggccgcggg ttgcggtcat accgactccg
     4561 tatgcccgat ggccacggcc atcctggtga acgacgacgg tccctgccgc ctgaaggagc
     4621 cgaagcggcc gtcgcaacag gtacagtcgc cggcgatcga tgggtcgacc agcccgagcg
     4681 cgtcggccag cgcccggttc gcctcgacga ggttcagccg gggccttccg tccggcctat
     4741 cgaccgctgt agagccgaat tccgactcga aggcgcgagc ccgggcctcg tcgaccacgt
     4801 agcaacacga acc
//
LOCUS       DAOPHV010000146         4789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_23856_length_4789_cov_4.290030, whole genome shotgun sequence.
ACCESSION   DAOPHV010000146 DAOPHV010000000
VERSION     DAOPHV010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23856_length_4789_cov_4.290030"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PLQ29_05145"
     CDS             <1..728
                     /locus_tag="PLQ29_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86066.1"
                     /translation="QLTAVLPECFSGLTYFFDPAEILKPCFYRLYKVEADVYLYLLRV
                     DLLPKPFDADTIEPGTNDRTSAYSTRRVYLESELIPLEAVMWEAGRVKAFRIKQLISQ
                     TWIGETGKGYLVRGVWMDSDLSKFFTKLFLPSGKRVYPFYPLFCKYKTLCCMPPLLSA
                     DGRRSLIPLLHKAIQFLAPEIGRIQEVLRNAKFDESLSDFKSLRDRVPESWKAPLAGY
                     SILAYLNDREQKEYSLDHAKDPT"
     gene            709..2097
                     /gene="murD"
                     /locus_tag="PLQ29_05150"
     CDS             709..2097
                     /gene="murD"
                     /locus_tag="PLQ29_05150"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPG86067.1"
                     /translation="MQKIRLEDIPGLRVTVMGLGLNGGGVASARFFAERGALVTVTDM
                     KDETALAESVAALSGLGIRYALGRHDEADFSGADLVIKNPAVRPDNPYLLAAKRVETD
                     LSMFLRFCPARIAAVTGSKGKSTTATAMAWMLKAGGLDAYLGGNITVSPLGFLDSATA
                     DSVVVLELSSWQLGDLRGSGVLKPRVAALTRIVPDHLDRYGTMEAYVADKRLIYADQD
                     SSDWTVFDLDDPYGRSFAAETGASVLGYGAILPPGTYGVRVPADGSPCIATLPDVGEA
                     ELVPERLLVPGAHARRNLAAAAVSAWALGVEPTRIAAAAATFPGVEHRLEPIRDRRGV
                     RWFNDSAATVPEAAKAAVEAFDAPVVLITGGTDKKLEFETARSAYARAASVVLLSGTG
                     TDKILPLLKADGVPYSGPYSDIGLAVAEADRLALPGSVVVLSPGCTSFGMFKNEFDRG
                     RRFKAAVAELPD"
     gene            complement(2125..2955)
                     /gene="amrB"
                     /locus_tag="PLQ29_05155"
     CDS             complement(2125..2955)
                     /gene="amrB"
                     /locus_tag="PLQ29_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPG86068.1"
                     /translation="MAIRARCLPDGWYPLSGAEAGAKLDAWARLDEQTRIGGAVAAIA
                     PHAGWYYSGRIAWSAWMSAGEADVVVIIGGHLPAGADFRMYREDGFDTPFGAAPADAE
                     LREVLARAVQVQGERGTDNTVEVHLPMAAHRFPGTPLVCLRAPNDERAARLGSELADY
                     QVSSGRRLFVLGSTDLTHYGKAYGFEPGGPAPGGFAWAGRANKDITDAFLAMDEAEAI
                     RQAELCGSACSVGAAVAAMSFARRLGASVSRLVMRGSSDELAPGSDSSVGYCAVTYSR
                     "
     gene            3008..4321
                     /locus_tag="PLQ29_05160"
     CDS             3008..4321
                     /locus_tag="PLQ29_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPG86069.1"
                     /translation="MKREPLLIAICSFVVIGALVAWPVLRVAKALDSGEEAARASFSE
                     LRRGALNTIQSPEDSVGGVWRAKVADAWAAQPNLLAVVIRDPDGEVLYARPDSSPYYA
                     RSDEGLVFERPEGTTTRFSGPISAGMSVEALYVTLTQEAAFYPIRDGAVALASLLALL
                     VAWLLAAANAKPEATNKSLRESPEAVTDDWEPSIPELIPPESSLYAEDPFGDSTSSPE
                     PAADEPAADEPAAYEPVAEDAAADEPTARTAPPGPKSAFIGQNDLPEGCLEGPRGLFD
                     AETGLGWEAYLRERLSAELKRSASFEQDLSLLIASIDGAERGDEEFNLFASSVRDYFT
                     FKDMAFLFGDNGAALILPNMDVDHALRMCGELMKKLSASMAVRRAGGTGVYMGLSSRA
                     GRLVDADRIIGESMAALSKARSDGQGGIMAFRPDPEKFRAYLAGQ"
     gene            complement(4322..>4789)
                     /locus_tag="PLQ29_05165"
     CDS             complement(4322..>4789)
                     /locus_tag="PLQ29_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86070.1"
                     /translation="VASGMASYALGRRSSWLDLRAWSSPPTPDDVEEVLLRGSLDGAG
                     GSAVSLDYMIARSGKGWMSGGEALDAMKVDAMIRAIAALRGDDYADADEPAGNAVVEL
                     ELRLGNGRTLNLAVEPVRADGRYPARSSQRERGMYLPAWSLTEALKPIGELRP"
BASE COUNT          833 a         1651 c         1512 g          793 t
ORIGIN      
        1 gccagctcac ggccgttttg cccgaatgct tctcgggcct tacctacttc ttcgacccgg
       61 ccgagatcct gaagccatgc ttctaccgac tctacaaagt cgaggccgac gtctacctct
      121 acctgctcag ggttgacctc ctccccaagc cgttcgacgc cgataccatc gagcccggaa
      181 ccaacgaccg gacttccgcc tactcgacgc ggcgagtcta cctcgagtcg gagctgatcc
      241 cgctcgaggc ggtcatgtgg gaggccggca gagtcaaggc cttcaggatc aagcagctga
      301 tctcgcagac ctggataggt gagaccggca agggctacct cgtcaggggc gtctggatgg
      361 acagtgacct gtcgaagttc ttcacgaagc tgttcctgcc gtccggcaag cgcgtttatc
      421 cattctaccc cctgttctgc aagtacaaga cgctctgctg catgccgccc ctgctcagcg
      481 ccgacggtag gcggagcctc atcccgctgc tccataaggc tatccagttc ctggcccccg
      541 agataggccg gatacaggaa gtcctcagga acgccaagtt cgacgaatcg ctgtcggatt
      601 tcaagtcgct cagggacagg gtaccggagt cgtggaaggc tccgctcgcg ggctatagca
      661 tcctagcgta cctcaacgac cgtgaacaga aggagtactc gctcgaccat gcaaaagatc
      721 cgacttgaag acatcccagg gctccgcgta accgtgatgg gcctcggcct gaatggaggc
      781 ggtgtggcgt cggcgcgctt cttcgcggag cgcggcgccc tcgtcaccgt aaccgacatg
      841 aaggacgaga cggcgctcgc tgagtcggtg gcggccctct ccggcttagg catccgatac
      901 gcgctcggcc gccacgacga agccgatttc tccggcgccg accttgtcat aaagaatccg
      961 gccgtcaggc cggacaatcc gtacctgctc gcggcgaagc gggtcgagac cgacctgtct
     1021 atgtttctac gcttctgccc tgcgcggata gccgcggtga cgggctccaa gggcaagtcg
     1081 accaccgcga ccgcgatggc gtggatgctc aaagctggcg gcctcgacgc ctatctcggc
     1141 ggcaacataa ccgtctcgcc gctcggcttc ctcgatagcg ccacggccga ctcggtcgtc
     1201 gtgctcgagc tgtcgagctg gcagctcggc gacctccgcg gctccggagt cctcaagccc
     1261 cgcgtcgccg ccctgacccg catcgtgccc gaccacctgg accgatacgg cacgatggag
     1321 gcctacgtcg ccgacaaacg gctcatctac gctgatcagg actcgtccga ctggaccgtg
     1381 ttcgatctcg acgaccccta tggacgttcc ttcgcggccg agaccggagc cagcgtgctt
     1441 ggctacgggg cgatcctgcc gcccggaacc tacggcgtac gggtgccggc cgacgggagt
     1501 ccctgcatcg ctacgctgcc ggatgtcggc gaggcggagc tcgtcccgga gcggctcctc
     1561 gttcccgggg cccatgccag gcgcaacctg gccgccgcgg cggtctccgc ttgggccctc
     1621 ggcgtcgagc cgaccaggat cgccgcggcc gcggctacgt tccctggcgt cgagcatcgg
     1681 ctcgagccca tacgcgatag gcgcggcgtc cgctggttca acgattcggc ggccaccgtg
     1741 cccgaggccg cgaaggcggc cgtagaggcg ttcgacgctc ccgtcgtgct cataacaggc
     1801 ggcaccgaca agaagctcga gttcgagacg gcccgctccg cgtacgccag ggccgcctcg
     1861 gtcgtgcttc tttcgggaac gggtaccgac aagatactac cgctactcaa ggccgatggt
     1921 gtgccgtaca gcggccccta ttccgatatc ggactggccg tcgccgaggc ggatcgcctg
     1981 gcgcttcccg gttctgtcgt ggtgctatcg cccggatgca cttcgttcgg catgttcaag
     2041 aacgaattcg accgcggcag gcgcttcaaa gccgccgtgg ccgagctacc ggactgacgg
     2101 gccgtaagcc ctggcaggcg ccagctagcg ggagtacgtc acggcgcaat aaccgacgga
     2161 cgaatcgcta ccgggcgcca gctcatcgct cgagccgcgc atgactaggc gggagacgct
     2221 ggccccgagt cgcctggcga atgacatggc ggccacggct gccccaaccg agcaggcgga
     2281 gccgcacaac tccgcctgtc tgatagcctc ggcttcgtcc atggccagga aggcgtcggt
     2341 aatatcctta ttggcgcgcc cggcccaggc gaagccacct ggagccgggc ctcccggctc
     2401 gaagccgtag gccttgccgt aatgcgtaag gtcggtggat ccgagcacga agaggcggcg
     2461 gccagacgag acctggtagt cggcgagctc cgagccaagc cgagcggccc tctcgtcatt
     2521 cggggcgcgc aggcatacga gcggagtccc ggggaatcga tgggcagcca taggcaggtg
     2581 cacctcgacg gtgttgtcgg tgcctcgttc gccttggacc tgtacggcac gggccagaac
     2641 ctcgcgaagc tcagcgtccg cgggggcagc gccgaacggc gtgtcgaagc cgtcttcccg
     2701 gtacatcctg aaatcggcgc ccgccggcag gtgcccgcca atgatgacga cgacatcggc
     2761 ttcacctgcc gacatccagg ccgaccaggc tatcctgcca gaataatacc atccggcgtg
     2821 gggcgcgatg gccgctaccg cgccgccgat tcgagtctgc tcatccagcc tagcccaggc
     2881 gtcgagcttt gctccggcct cggcccccga taaaggatac catccgtccg gcagacaacg
     2941 ggcgcgtata gccatggggc ctcctctcgg ttccaaaaaa gggatattag gattatactg
     3001 catcactatg aaacgcgaac cattacttat agccatatgc tcgttcgtcg ttataggcgc
     3061 tctcgtcgcc tggccggtcc tacgcgtcgc caaggccttg gactcgggag aagaagcggc
     3121 gcgcgcctca ttctccgagc ttcgccgcgg cgcgctgaac acgatccagt ctccggagga
     3181 ttcggtcggc ggcgtctgga gggccaaggt cgccgacgcg tgggcggctc aaccgaacct
     3241 tttggccgtc gtcatcagag acccggacgg cgaggttctg tacgccagac cggactcgtc
     3301 accatactac gctcgaagcg acgaggggct agtcttcgag cgtcccgaag gcacgaccac
     3361 gcgtttcagc ggccccatct ccgcgggcat gagcgtcgag gcgctctacg tgaccctgac
     3421 gcaggaagcc gcattctacc cgatccgcga cggagccgtg gccctcgcct cgctcctggc
     3481 cctactggtc gcatggctcc tcgcggcggc gaacgccaag ccggaagcta cgaacaagag
     3541 tctccgcgag tcgccggaag ccgttaccga cgattgggag ccaagcattc cggagctcat
     3601 accgcccgag tcgtcgctat acgccgagga cccgttcggc gacagcacat catcgcccga
     3661 accagcggcc gacgaacccg cggccgacga acccgcggcc tacgaacccg tggccgaaga
     3721 tgccgcggcc gacgagccga ccgcgagaac cgctcctccc gggccgaagt ccgcgttcat
     3781 cggccaaaac gacctgccgg aaggttgcct cgagggccca cgcggactgt tcgacgccga
     3841 gaccggcctc ggctgggaag cctacctgcg agagcgactc agcgccgagc tcaagcgctc
     3901 cgcctcgttc gagcaggacc tatcactgct gatcgcgtcc attgacgggg ccgagcgcgg
     3961 cgacgaggag ttcaacctct tcgcgtcgtc cgtgcgcgat tactttacct tcaaggacat
     4021 ggctttcctg ttcggggaca acggagccgc gctcatcctg ccgaacatgg acgtggacca
     4081 cgcgctcaga atgtgcggcg agctgatgaa gaaactgtcc gcgtcgatgg cggtcaggcg
     4141 agccggcggg accggcgtct acatgggcct gtcctcgagg gctggacgcc tcgtcgacgc
     4201 cgaccgtatc atcggcgagt cgatggccgc cctgtccaag gcgcgctccg acggccaggg
     4261 cggcatcatg gccttccgcc ccgatcctga gaagttccgc gcctatctcg ccggacaata
     4321 gctagggccg tagctccccg atcggcttga gggcctcggt aaggctccag gccggcaggt
     4381 acatcccgcg ctcccgctgc gacgaccggg ccggatagcg cccatcggcg cgaaccggct
     4441 cgacggccag gttcagcgtc ctgccgttcc cgagtcgcag ctccagctcg acgaccgcgt
     4501 ttcccgcggg ttcgtcggcg tcggcgtaat cgtcgccgcg tagcgcggcg atcgcccgaa
     4561 tcatcgcgtc gaccttcatt gcgtcgagag cctcgccgcc gctcatccag ccctttccgg
     4621 atctcgcaat catataatcg aggctcacgg ctgagccgcc agcgccgtcg agcgagccgc
     4681 ggagcagtac ctcctcgacg tcgtcggggg tcgggggcga cgaccaggcc cgaaggtcga
     4741 gccagctcga gcgacggccg agcgcgtagg aggccatgcc cgaggcgac
//
LOCUS       DAOPHV010000147         4785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_23883_length_4785_cov_4.002748, whole genome shotgun sequence.
ACCESSION   DAOPHV010000147 DAOPHV010000000
VERSION     DAOPHV010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23883_length_4785_cov_4.002748"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLQ29_05170"
     CDS             <1..518
                     /locus_tag="PLQ29_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPG86071.1"
                     /translation="LMLDPRGLANQAVNWIAMTPGIDHQWLSSATMVQVSTMVVYVWK
                     YLGYFTILFVTGIGKIPDSVNEAAMIDGAGPVRRFFSITLPLLRPTTVLVSVMILIQC
                     MKTFSTQYLFTQSGAPLAPINVITLNIYNTAMRDLNIGRASVMSTILFLFMLALTWVQ
                     LRVSKGDEAES"
     gene            515..1369
                     /locus_tag="PLQ29_05175"
     CDS             515..1369
                     /locus_tag="PLQ29_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPG86072.1"
                     /translation="MRRGIKILDRVGGAAGAVLVAAACAVVVVPLVFMIGSSFMPAMD
                     IMQMPYRWIPETWKPANYLRALAGNDGQWMYLRNILNSVIVSTTIAITTVVLSSMVGY
                     GLAKFRFRGRNAVFMAIMGTMMIPFETVMIPLYLIIVKAGLQNSYAGLILPFLMNAFG
                     VFIMRQYLVTFPDEMLDAARIDGCSEPRIFRDMVFRNSGPAAATLAILSFRQQWDNLL
                     WPLLVAQEERMKTIPAYIVKFTAEKHADEGAMMAVAVIASLPVFVLFFTLSKYFLGGS
                     AVYQAGKE"
     gene            1366..1971
                     /locus_tag="PLQ29_05180"
     CDS             1366..1971
                     /locus_tag="PLQ29_05180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPG86073.1"
                     /translation="MSLFIFDMGGVMVGSFDVLPEAALRLGLPETELRRLAAPDLDEL
                     MAGRMAADDYWARFSVASGLSVGEDYWSTLFAPSLDPVMVGLARSLRRRGRVVCGTNT
                     IASHYDYLRATGMYDVFDAVYASHLMGVCKPDPDFWRLILEAEGVAAPDAVFVDDMAE
                     NAEAAGALGLRAHLFDGVAGLEAFLDRLDDEPEAADRRLEA"
     gene            1968..3011
                     /locus_tag="PLQ29_05185"
     CDS             1968..3011
                     /locus_tag="PLQ29_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPG86074.1"
                     /translation="MTRRSAHGRIGLVVVVAAALAASCARADPVKAVPEADLVVFSSH
                     PDELAKTIIDEFRERTGLRVRLVLDGTGALLSRLSAGERADVLWGGGAESLVANLGLF
                     QPYLSPERDAVPAALKDEDGYWTGLTVLPMVIMVNARLVPPEAVPAGWSDLLAPELAG
                     GVAFANPVSSASSYTILRTMMVAARARAGGDQDSDWRFVEGFIDAIGGSTLSESAMVY
                     RVVAAGEYLVGLTYENAGAESMRLGSDVRIVYPADGTSTVPDGVAILRDAAHDEAARR
                     FIDFVLGHDVASVVSTRFKRRSARVDVPAPDGLPPLESVRLLDYDFDAAVTEREATMA
                     RFTTMLQEAGQRR"
     gene            complement(3008..3832)
                     /locus_tag="PLQ29_05190"
     CDS             complement(3008..3832)
                     /locus_tag="PLQ29_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPG86075.1"
                     /translation="MRRLVIAEDEELARRELETTMPWERWGYMLVGSAEDGLHAWDLV
                     VERRAEVVLTDIRMPGLDGLALMRKAEAELAPLERPLFIIVSGHADFDYAKEAVRLGA
                     FDYLIKPVDDGELEDAMLRAAKRAGERERLAGLERAATADQAYGLLRDLTRERTAESG
                     DSYVEAAIADLGERFMTDLSADSVAGRLGISGDHLSRLLKRATGLTFNEYLTRLRMKR
                     AMELLRDPTVRVGEVADLSGYRDARYFSTLFRRVIGMTPSEFRSGRIRRDTGDPSP"
     gene            complement(3829..>4785)
                     /locus_tag="PLQ29_05195"
     CDS             complement(3829..>4785)
                     /locus_tag="PLQ29_05195"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPG86076.1"
                     /translation="AEAPSGLYDGFGRSARNVALAGLLAAAGLASVLSLRASASVTEP
                     VLEMARSMRLVEDGDLSIRIKPSGDDELGDLARSFNAMTAEVDALLRREVERQELLRE
                     AELRALAAQMNPHFLHNTLASIKSLAKLGRSGEIAEVVSRLGKLLRSGSGRRDGLSTV
                     SESLSSVRDYLSIEKLRFGERFSFELDVDPALESVTLPPLTLEPLAENALTHGLERKR
                     GPGSLRIEGRLDGGDAVISFADDGPGMDAEAIARLSEALEEAKAPAGEHGMGLLGTNR
                     RLALEYGAGYGIRIRNGGPSGAGFVATVRIPSGLATPKGEAP"
BASE COUNT          749 a         1619 c         1583 g          834 t
ORIGIN      
        1 tcctgatgct cgaccctcgc ggccttgcca accaggcggt caactggata gcgatgacgc
       61 cgggaataga ccaccagtgg ctgtcgagcg cgacgatggt ccaggtctcg acgatggtcg
      121 tgtacgtctg gaagtacctg ggctatttca ccatcctgtt cgtcaccggc atcggcaaga
      181 tacccgattc ggtcaacgag gcggcgatga tagacggcgc ggggccggtc cggcgcttct
      241 tctcgatcac cctgccgctc ctcaggccga ccaccgtgct ggtctcggtg atgatactga
      301 tccagtgcat gaagaccttc agcacccagt acctgttcac ccagagcggg gcgccgctgg
      361 ccccgatcaa cgtgatcacg ctgaacatct acaacacggc catgcgcgac ctcaacatag
      421 gcagggcgag cgtgatgagc accatcctgt tcctgttcat gctcgcgctg acctgggtcc
      481 agctgcgcgt gtccaagggc gacgaggccg aatcatgagg cgcggtatca agatcctcga
      541 ccgggtaggc ggcgccgccg gggcggttct ggtcgcggcg gcctgcgccg tcgtcgtcgt
      601 gccgctcgtc ttcatgatag gctcgtcgtt catgccggcg atggacatca tgcagatgcc
      661 gtaccgctgg ataccagaga cctggaagcc ggccaactac ctgcgcgcgc tcgccggcaa
      721 cgatggccag tggatgtacc tgcgcaacat cctcaactcg gtcatcgtct cgacgacgat
      781 cgcgatcacg acggtcgtgc tgtcgtcgat ggtcggctac ggcctggcga agttcaggtt
      841 caggggtcgg aacgccgtgt tcatggccat catgggcacc atgatgatcc ccttcgagac
      901 ggtgatgata ccgctgtacc tgatcatcgt gaaggcgggg ctccagaaca gctacgccgg
      961 cctgatcctg cccttcctga tgaacgcctt cggggtgttc atcatgcggc agtacctggt
     1021 caccttcccc gacgagatgc tcgacgccgc caggatagat ggctgctcgg agccgcgtat
     1081 cttccgcgac atggtcttca ggaattccgg ccccgcggcc gcgactctgg ccatcctgtc
     1141 gttcaggcaa cagtgggaca acctcctatg gccgctcctc gtcgcgcagg aggaacggat
     1201 gaagaccata ccggcctata tcgtcaagtt caccgcggag aaacacgccg acgaaggcgc
     1261 gatgatggcc gtggcggtca tcgcctcgct accggtcttc gtgctattct tcaccctatc
     1321 caagtatttc ttagggggat cggccgtcta ccaggccggt aaggaatgag tctcttcatc
     1381 ttcgacatgg gcggcgtgat ggtcggctcg ttcgacgtac tacccgaggc cgcgctcagg
     1441 ctaggcctgc ccgagaccga gctacggcgc ctggcggcgc ccgacctgga cgagctaatg
     1501 gccgggcgca tggctgccga cgactactgg gcgcggtttt cggtcgcatc cgggctgagc
     1561 gtcggcgagg actactggag cacgctgttc gcgccatcgc tcgacccggt gatggtcggc
     1621 ctggcccgta gcctacgccg ccggggccgc gtggtctgcg gcaccaacac gattgcttcg
     1681 cattacgact acctccgggc caccggcatg tacgacgtct tcgacgcggt ctacgcgtcc
     1741 cacctgatgg gcgtctgtaa gccggatccc gatttctggc ggctcatcct tgaagcggag
     1801 ggcgtagcgg ccccggacgc ggtcttcgtc gacgatatgg ccgagaacgc ggaggcggcg
     1861 ggcgccctcg ggcttcgcgc ccacctgttc gacggcgtgg ccgggctcga ggcgttcctg
     1921 gatcggctcg acgacgagcc cgaggcggca gaccggaggc tcgaggcgtg actcgccgct
     1981 cagcgcacgg tcggatcggc ctcgtcgtcg tcgttgcggc agcgttggcc gcctcgtgcg
     2041 cccgcgccga tccggtcaag gccgttcccg aggccgacct ggtcgtcttc tcgtcccacc
     2101 ctgacgaact cgccaagacc atcatagacg aattcaggga gcgcaccggg cttcgggtaa
     2161 ggcttgtatt ggacggcacc ggcgcgctgc tctccaggct atcggctggc gagcgggccg
     2221 acgtgctctg gggcggcggc gccgagagcc tcgtggcgaa cctggggctg ttccaaccct
     2281 acctgtcgcc ggaacgcgac gccgtcccgg ccgcgctcaa ggacgaggac ggctactgga
     2341 ccggcctgac cgtgctaccg atggtcatca tggtcaacgc gcgacttgtg ccgcccgagg
     2401 ccgtaccggc agggtggtca gacctgctgg ctccggagct agcaggcggc gtggcgttcg
     2461 cgaaccccgt ctcttccgcc agctcctata ccatcctgcg cacgatgatg gtcgcggcca
     2521 gggctcgggc ggggggcgac caggactccg actggcgttt cgtcgagggc ttcatcgacg
     2581 ccatcggcgg ctcgaccctg agcgagtcgg ccatggtata ccgcgtcgtg gccgcgggcg
     2641 agtacctggt cgggctgacc tacgagaacg ccggggccga gtcgatgcgc ctcggctcgg
     2701 acgtcaggat agtctatccg gccgacggca cctcgaccgt gcccgacggc gtggccattc
     2761 tgcgagacgc cgcccacgac gaggcggcca gacgcttcat cgacttcgtc ctcggccacg
     2821 acgtggccag cgtcgtttcg acccgcttca agcggcgttc ggcccgtgtg gacgtgccgg
     2881 caccggacgg tctgccgccg ctcgagagcg tgcggttgct cgactacgac ttcgacgctg
     2941 cggtgactga gcgcgaggcg acgatggcgc gcttcacgac gatgctccag gaagccggcc
     3001 aaaggcgcta gggcgatggg tcccccgtgt cgcggcgtat cctgccgcta cggaattcgc
     3061 tcggggtcat gccgatgacc cgcctgaaca gagtgctgaa atagcgcgcg tcacggtacc
     3121 cggacaagtc cgccacctcc ccgacgcgca cggtaggatc gcgtaagagc tccatcgccc
     3181 tcttcatgcg cagtcgtgtc aggtactcgt tgaaggtcag gcccgtggcc cgcttgagca
     3241 gcctggacag gtggtcgccg gagatgccga gccgcccggc gaccgagtca gccgacaggt
     3301 cggtcatgaa gcgctcacca aggtcggcga tggccgcctc cacgtacgag tcgcccgatt
     3361 cggccgtacg ctcccgggtt aggtcgcgta ggaggccgta ggcctggtcg gccgtcgccg
     3421 cccgctcgag cccggccagc cgttcgcgct ccccggcgcg cttggccgcc cggagcatcg
     3481 cgtcctccag ctcgccatcg tcgacgggct tgatcagata gtcgaacgcg ccaagtcgta
     3541 ccgcctcctt cgcgtagtcg aagtcggcgt gcccggaaac gatgatgaat agcggccgtt
     3601 ccagaggcgc cagttccgcc tcggccttgc gcatcagggc gagcccatcg agtcccggca
     3661 tgcgtatgtc ggtgagcacc acctcggctc ggcgctcgac gacgaggtcc caggcgtgga
     3721 ggccgtcctc agccgagcct acgagcatgt agccccagcg ttcccagggc atcgtcgtct
     3781 cgagctccct gcgggcgagt tcctcgtcct cggctatgac gagccgcctc atggggcctc
     3841 ccccttggga gtcgcgagtc ccgacgggat cctgaccgtc gcgacgaagc ccgcgccgga
     3901 cggtccgccg ttccgtatcc tgatgccgta gcctgcgccg tactcgaggg ccaggcgtcg
     3961 gttcgtgccc aggagtccca tgccgtgttc gcccgccgga gctttggctt cttcgagggc
     4021 ctccgatagc cgggctatcg cctcggcatc catacctgga ccgtcgtcgg cgaaggagat
     4081 cacggcgtcg ccgccatcca accggccctc gatcctcagg gatccgggcc cgcgcttacg
     4141 ctccagcccg tgcgtcaggg cgttctccgc gagtggctcg agcgtcagcg gtggtagcgt
     4201 cacggactcc agggccgggt cgacgtccag ctcgaagctg aagcgctctc cgaagcgaag
     4261 cttctcgatg gagaggtaat cgcgcaccga cgataggctc tcgctcacgg tggacaggcc
     4321 gtcgcgtcgc ccggaaccgg atcgcaggag cttgccgagc ctggagacta cctccgcgat
     4381 ctcgccggaa cggcctagct tggccagcga cttgatcgag gcgagcgtgt tgtggaggaa
     4441 gtgcgggttc atctgggccg ccaaggcgcg tagctccgcc tccctgagca gctcctgacg
     4501 ctccacttcg cgccggagga gcgcgtccac ctcggcggtc atcgcgttga acgagcgggc
     4561 caggtcgcct agttcgtcgt ccccggaagg cttgatacgg atcgagaggt cgccgtcctc
     4621 gacgaggcgc atcgagcggg ccatctccag caccggctcg gtaactgacg ccgaggcgcg
     4681 cagggacaga accgacgcca ggcccgccgc ggcgagcagc ccggccagcg cgacgttgcg
     4741 cgccgagcgg ccgaagccgt cgtagaggcc gctcggcgcc tcggc
//
LOCUS       DAOPHV010000148         4726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_24277_length_4726_cov_4.111753, whole genome shotgun sequence.
ACCESSION   DAOPHV010000148 DAOPHV010000000
VERSION     DAOPHV010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24277_length_4726_cov_4.111753"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="PLQ29_05200"
     CDS             <1..1668
                     /locus_tag="PLQ29_05200"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86077.1"
                     /translation="SFDIAVADRVGAVSILVEAAGPGGRVSNQRVDVPIRSASVPVTT
                     VSSALIAGKATATVNAALPGMPGSNEAWLELSLVPPVDLSGRLAYLIGYPHGCGEQTV
                     SKAFPQLFLADAVALSPEQAEEARSNVAAAITKMAGFQTASGGFVFWQGSYDESAWLT
                     AYTTHFLAMARKQGYSVPDAMLGAAIAKIRGQASGWNAQADYSKAEQAYRLYVLALAG
                     KPDVASMNRFMEYGPHQAAALYQVAAAYALAGMSDRARSILRDGPADVTRYDGMRRVY
                     GSVLRDRAIVLDAFNALGDQARGLPLFKRIAEDLSSGSGYSTQDLSYALIASLPYMKA
                     ASSGAATVRYSYDGVSGSAAITKAITRVQLRAGADPLSVHLENASGAAVYARVAITGT
                     PKPGSEPYRSEGLQLSARYLDASEQSVDPARLPFGSDMIVELAVRNTSGDELSDVALT
                     FRAASGWEIANLRVGKTGDDGSSGSSYDYQDTRDDRVMTYLGLKRGETKRFRFYVNKT
                     YEGEFFLPAFTAEAMYDPTVFAVLPGRPLPKPVAEQRANPNSRGQRP"
     gene            1686..4088
                     /gene="pbpC"
                     /locus_tag="PLQ29_05205"
     CDS             1686..4088
                     /gene="pbpC"
                     /locus_tag="PLQ29_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HPG86078.1"
                     /translation="MALAWATTAAKARRGATIVLAWAARPDTAPALAGAALFVLLFMA
                     FEPPTLAPAAYSSALYAASGELLGAKVAPDGQWRFEPSGEVPERYAVALVEYEDGRFY
                     GHAGFDPLAIARAARLNARSGRVVSGGSTVTMQAARLSKAPGPRDLGAKIVELWTAVR
                     LEFGQTKAEILAGYAGMAPYGGNVVGLEAAGFRWFGRPSEALTWAEAATLAVLPNSPG
                     LVHPGRSRDELHRKRDRLLSSLADAGRIDAEALAVALAEPLPEEPLPMPDLAPHLLAK
                     TGPGRTVSTIDGRIQTVAVEIASRHSRRLGRNGVANLAIVVLRVDDGSVAAYVGNSPG
                     AGADEARWVDCADSPRSSGSILKPFLYAAMIDSGELAPGRLVLDIPTRVGSYSPENNL
                     KTYSGAVRADEALARSLNVPFVRLLRSYGVERFASLLKRLGFSTLSRAPADYGLPLIL
                     GGAEATLIDAASAYASLARMAKADDPAPPVQSAGATWRSGDDAGDDAGDGAGRSMFEP
                     QFSAGAAWLTLEALVSVARPGEEASWQEFASSRRVAWKTGTSFGSRDAWAIGATPEYV
                     VAVWAGNADGSGRPELKGTDAAAPILFDTFQALPRSGWFAEPVRSLRWETVCAESGYP
                     AGPDCATTDRVLVPAASKADQPCPYCRTVSLSADGRYRVRAEEVGGAELLVERRFALP
                     PAVEWYYARSSPGYRPLPPWLPGFAGAEADEIEFLAPELGASLYVPIELDGTPGKAVF
                     RAAHRDRAARLFWHLDGVYLGETVVDHRVEARPAPGFHELVVVDGHGSTASRRFEVYD
                     RE"
     gene            4373..>4726
                     /locus_tag="PLQ29_05210"
     CDS             4373..>4726
                     /locus_tag="PLQ29_05210"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPG86079.1"
                     /translation="MEIRETLSYDDVLLEPGYSAILPKDASVRSRLAGELYLNAPILS
                     AAMDTVTESELAIALALQGGAGVIHRNLSPADQADQVASVKRFLNWIIESPVTVEEDR
                     TIRDVRAIMDRHRVTG"
BASE COUNT          696 a         1699 c         1598 g          733 t
ORIGIN      
        1 tcgttcgaca tagccgtcgc cgaccgggtc ggcgcggtca gcatcctggt ggaggcggcc
       61 ggccccggcg gccgggtctc gaaccagcgc gtcgacgtgc ccatccggtc ggcctccgtg
      121 ccggtcacga ccgtcagctc cgccctgatc gccggcaagg ctacggccac cgtcaacgcg
      181 gcgcttcccg gcatgcctgg ctcgaacgag gcctggctcg aactgtcgct cgtgccgccg
      241 gtcgacctgt ccggtcgtct cgcctatctc atcggctatc ctcacggctg cggcgagcag
      301 acggtcagca aggccttccc gcagctgttc ctggccgacg ccgtcgcctt gagccccgag
      361 caggccgagg aggccaggtc gaacgtcgcg gccgccatca cgaagatggc gggctttcag
      421 accgcctcgg gcggcttcgt cttctggcag ggttcgtacg acgagagcgc ctggctgacg
      481 gcctatacga cccatttcct ggccatggcc aggaagcagg gctactccgt gccggacgcg
      541 atgctcggcg cggctatcgc caagatacgc gggcaagcct ctggctggaa tgcccaggcc
      601 gactattcga aggccgagca ggcctaccgg ctctacgtgc tcgccctcgc cggaaagccc
      661 gacgtcgcgt cgatgaaccg cttcatggag tacggaccgc accaggccgc ggccctgtac
      721 caagtagccg cggcctacgc cctggccggc atgagcgacc gcgccaggtc gatcctgcgc
      781 gacgggcctg ccgacgtgac caggtacgac ggcatgcgcc gggtctacgg ctcggtgcta
      841 cgtgaccgag ccatcgtgct cgacgcgttc aacgctctcg gcgaccaggc ccgcggcctg
      901 ccgttgttca agcgcatcgc cgaggacctg tcgtccggct caggctactc gacccaggac
      961 ctgagctacg cgctgatcgc ctcgctcccg tatatgaagg cggcctcgag cggcgcggct
     1021 accgtacgct atagctacga cggcgtgtca ggtagcgctg ccatcaccaa ggccattacc
     1081 agggtgcagc tacgcgccgg tgcggatccc ctgtcggtac acctcgagaa tgcctcaggg
     1141 gccgcggtct atgcccgcgt cgcgatcacc ggtacgccca agccgggtag cgagccgtac
     1201 cgctccgagg gcctgcagct ctcggcccgc tacctcgacg cctccgagca atcggtcgat
     1261 ccggccaggc tacccttcgg tagcgacatg atagtcgaac tggccgtgcg caacacctcg
     1321 ggtgacgagc tgtccgacgt agctctaacc ttcagggcgg cctccggttg ggagatcgcg
     1381 aacctgcgcg tcggcaagac cggtgacgac ggttcgtcgg gctcgagcta cgattaccag
     1441 gatacgaggg acgacagggt catgacctac ctcggcctca agcgcggcga gaccaagcgc
     1501 ttccgcttct acgtgaacaa gacctacgag ggcgaattct tcctaccggc cttcaccgcc
     1561 gaggcgatgt acgacccgac ggtgttcgcg gtgctgccgg gccggccctt gcccaagccc
     1621 gtcgccgagc agcgggccaa cccgaatagc cgcggccaaa ggccttgaac cgtaggcttg
     1681 cgccgatggc gctggcctgg gcgacgacgg ccgccaaggc ccggcgcggt gcgacgatag
     1741 ttctggcctg ggcggcccgg cccgacacgg ccccggcctt ggccggggcc gccctgttcg
     1801 tcctcctgtt catggcgttc gagccgccga ccctggcgcc ggcggcgtat tcgagcgccc
     1861 tgtacgccgc gtcgggcgag ctactcgggg ccaaggtggc tccagacggg cagtggcggt
     1921 tcgagccgtc gggcgaggtg cccgaacgct acgcggtcgc cctcgtcgag tacgaggacg
     1981 ggcgcttcta cggccacgcc ggcttcgacc cgctggccat cgcccgagcc gccaggctca
     2041 acgccaggag cggccgcgtg gtctccggag gatcgaccgt caccatgcag gccgccaggc
     2101 tgtccaaggc tcccgggccg cgcgatctcg gggccaagat cgtcgaatta tggaccgccg
     2161 taaggctgga attcggccaa accaaggcgg agatcctggc cgggtatgcc ggcatggcgc
     2221 catacggcgg taacgtcgtc gggctcgagg ccgccggttt ccgctggttc ggccggcctt
     2281 ccgaggcgct cacctgggcc gaggccgcca cgctcgccgt gttgccgaac agtcccggcc
     2341 tcgtgcaccc cggccgctcg cgggacgagc tgcaccgcaa gcgggaccgg ctcctgtcct
     2401 ccctggccga cgcaggccgt atcgacgccg aggcgctcgc cgtcgccctg gccgagcccc
     2461 tgcccgagga gcccctcccc atgcccgacc tcgcgcccca cctgcttgcc aaaaccggcc
     2521 cggggcgcac cgtctcgacc atcgacggca ggatccagac cgtggcggtg gagatcgcct
     2581 cgaggcactc gaggcgcctc gggcggaacg gcgtcgccaa tctcgccatc gtcgtcctac
     2641 gcgtcgacga cggtagcgtc gcggcctacg tcggcaactc gccaggggcc ggggctgacg
     2701 aggcccgctg ggtggactgc gccgactctc cccgtagctc ggggagcatt ctcaagcctt
     2761 tcctgtatgc tgccatgatc gactcggggg agctagcgcc cggccgcctt gtcctcgata
     2821 taccgacgcg ggtcggctcg tacagcccgg agaacaatct caagacctat tcgggcgcgg
     2881 tgcgggccga cgaggcgctt gccaggtcgc tcaacgtgcc cttcgtcagg ctgctccgct
     2941 cgtacggagt cgagcgtttc gcctcgctcc tcaagcggct cggcttctcg acgctgtcga
     3001 gagcgccagc cgactacggt ctgcccctga tactcggcgg cgcagaggcg accctcatcg
     3061 acgcggcctc ggcctacgcc agcctcgcgc ggatggccaa ggccgacgat cctgcgccgc
     3121 ccgtgcagtc tgccggcgct acctggcgtt cgggcgacga cgcaggcgac gacgcgggcg
     3181 acggcgcggg ccgctccatg ttcgagccgc agttttcggc cggcgcggcc tggctaacgc
     3241 tcgaggccct cgtgagtgtg gccaggccag gcgaggaggc ctcctggcag gaattcgcca
     3301 gctcacggcg cgtcgcctgg aagaccggca ccagcttcgg ctcgcgcgac gcctgggcca
     3361 taggcgcgac gccggagtac gtcgtcgccg tctgggcggg caacgccgac ggctcgggcc
     3421 gaccggagct caagggcacc gacgccgccg cgcccatcct gttcgatacg ttccaggcgc
     3481 taccccgctc gggctggttc gccgagccgg tgcgatccct gagatgggag acggtctgcg
     3541 ccgagtccgg ctatcccgct ggccccgact gcgcgacgac cgatcgcgta ctcgtaccgg
     3601 cggcctcgaa ggcagaccaa ccctgtccgt actgccggac ggtatcgctg agcgccgacg
     3661 gccgctaccg cgtcagggcg gaggaagtcg gcggcgcgga actgctcgtc gagaggcgtt
     3721 tcgcgttgcc tcccgcggtc gagtggtact atgctcggtc gagcccgggc tatcggccgc
     3781 taccgccctg gctgcccgga tttgccggag ccgaagctga cgagatcgag ttcctagcgc
     3841 cggagctcgg ggcgtcgctg tacgtgccga tcgagctcga cgggacgccg ggtaaggccg
     3901 tgttccgcgc ggcccatcgc gatcgggcgg caaggctgtt ctggcacctc gacggcgtct
     3961 acctcggcga gacggtcgtc gaccaccgcg tcgaggcgag gccggctccg ggcttccatg
     4021 agctggtcgt cgtcgacggt catggctcga ccgcctcgcg gcgcttcgag gtctacgacc
     4081 gggagtaacc ggtctagcgc gccggttcgc atccccgagc ggttcgtgcc tgccgggctt
     4141 gcgcccgtcg actattccga tgtataaccc gctatgcacc ccatcgaaca tttcagaggc
     4201 cccgtccgtc gacgccgaca gagcgtcggt ttgggcacgc ccggggctac gggacttggg
     4261 cgccggctct cgggagccgg acgcacctag gcggacacgc cgcgccgagc gcggtgggag
     4321 ggtccgcggg cgatcgtcgg cggacgccga tacgattgac cgaggaggcc ccatggagat
     4381 tcgcgagacc ctttcgtacg acgacgtgct gctcgagccc ggatattcgg cgatcctgcc
     4441 caaggacgcg agcgtccgat cgcgcctggc gggcgagctc tacctgaacg cgccgatcct
     4501 gagcgccgcg atggacaccg tcaccgagtc tgagttggcc atcgctctgg ctctgcaggg
     4561 cggagccggc gtcatccacc gcaacctgag ccccgcggac caggcggacc aggtcgcctc
     4621 cgtaaagcgc ttcctgaact ggatcatcga gtcgccggtc accgtcgagg aagaccgcac
     4681 catccgcgac gtgcgcgcca tcatggatcg ccaccgcgtc accggc
//
LOCUS       DAOPHV010000149         4705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_24451_length_4705_cov_4.055484, whole genome shotgun sequence.
ACCESSION   DAOPHV010000149 DAOPHV010000000
VERSION     DAOPHV010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24451_length_4705_cov_4.055484"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PLQ29_05215"
     CDS             <1..914
                     /locus_tag="PLQ29_05215"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HPG86080.1"
                     /translation="LRRSASLENVGDAFKVVAGYLAARRVLRGLRPALLFSKGGYVSV
                     PPCMAAAALGIPVFTHESDLSTGLATRLNARRAERILVSWERTLDDLPESQRARAIAV
                     GNPVRRAIRAGNAAAGRRWLGFADDLPIVLALGGSQGARQVNDLIGAILPALAGKARV
                     AHQCGPGNPVCRPADGDYKGFEFVSGELPDLYAAADVIVGRAGAGTLWEGAASGKPMV
                     FIPLGSGSRGDQVENARLLASRGGAVCLAGDEATPEALLAALTPLLSNPEARSTMAAA
                     AGETARPDAAAAIAGMILDRVLKGSTR"
     gene            911..1390
                     /locus_tag="PLQ29_05220"
     CDS             911..1390
                     /locus_tag="PLQ29_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPG86081.1"
                     /translation="MNVLDYVLLAVIAIAALRCWFRGIVGEVLSMAAVVGGLLAGIFF
                     FRPVGAWISNLKPLGGFEVVVGFVASFAVVFIVVKIVEKSLRSVLENLNLDILDSVLG
                     LIFGVVEGVIISAIVVMILHYQPVFDVEALLDGSWIARLLLPLVVEALPAARSSISG"
     gene            1396..2694
                     /locus_tag="PLQ29_05225"
     CDS             1396..2694
                     /locus_tag="PLQ29_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86082.1"
                     /translation="MFENILWQPNATEALKAELDRGALPGSLLFEGPPLSAKLSSALE
                     LSRALSCERDAAWNCPCQQCSRHRTLSHQDTLLLGPKSFREELAIGAAMMERAPGSGS
                     RFFFTRAVKKLTRRFDQELYAGEEARLSKALPLVRSALEGVDACAPASPTCRSSALSD
                     DEAVKEAGKLLGVCAKLEDLLPAATPVFQVRAMEFWARLAPYGRRKTIIIEHADRMLD
                     ASRNALLKILEEPPEHAVFVLTSSRKAALIPTVLSRVRPYRFAQRTGDEARAVIERIF
                     KDDAAGAPTLGEYVSRYRPSSTGAVDGLARDLAAAIVAAAERRRGPFTEEALSSLASS
                     GGPVQAAIAAAQSATANFGSADEAFAWAFPALMDSVGSIFAAMLRLPGTGLETQRAAE
                     LYASLARDAMIRYSSYNLQPTALVERLADAFADGCQDGRP"
     gene            2691..3896
                     /locus_tag="PLQ29_05230"
     CDS             2691..3896
                     /locus_tag="PLQ29_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG86083.1"
                     /translation="MRKFLERAMEKVSRMNEESIRGLLRVLADENERLDAVLDSMLDG
                     IVVCDEDHAPILVNKAAERLLPMAQAELFDLPLWESVRDDELAGFFDAALNGEESVID
                     REFALDAKAGTRIISVSVTPLVRDKRIRGTLIHVEETTDKRRREARLRRAENLASLTT
                     LAAGVAHEIKNPLGSISIHVQLMRKALKGLADEPPIGRYLDVVTEEIDRLNKIVVDFL
                     FAVRPMDIAPINDDLNALLRELAEFMRVEVESAGIELSVDYGKDVPPFPFDRRYLKQA
                     LLNLVKNAAAAMPDGGSLRLTSEASAEEARITVSDTGVGITEDRLGKIFEPYYTTKEN
                     GTGLGLTLTYKIVKEHGGDITVTSKAGQGSTFVLALPIPQRDRRMITGACPDGEACEH
                     EPLPDTEEG"
     gene            3893..>4705
                     /locus_tag="PLQ29_05235"
     CDS             3893..>4705
                     /locus_tag="PLQ29_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPG86084.1"
                     /translation="MRFTVLVVDDEKNIREGLGEYLRLDGYDVELAADGREGVALADR
                     GGVDLVITDLRMPALSGGELLRHVVSRYPSVPVIVLTGHGTVEDAVDAMRSGAYDFIT
                     KPVNLDHLSILVKRALERRELSRRNEELLEEIETQRRTSSIIGRSPAISRIMELVRRV
                     APTKASVLITGESGVGKELVADAIHNLSPRADGPFIKVHCAALAESLLESELFGHEKG
                     SFTGAQARKKGRFELANEGTLFLDEIGEINQNVQIKILRVLQDKRFERVGGEE"
BASE COUNT          790 a         1622 c         1550 g          743 t
ORIGIN      
        1 agctccgccg atcggcgtcg ctcgagaacg tcggcgatgc cttcaaggtg gtcgctggct
       61 acctggccgc gaggcgcgtt ctacgcgggc tacggccggc cctgctgttc tccaagggcg
      121 gctatgtcag cgtgccgccg tgcatggcgg cggcggcgct cggcataccg gtgttcacgc
      181 acgagtcaga cctgtcgacc ggcctggcga cgcggctcaa cgccaggcgg gccgagcgca
      241 tcctggtcag ctgggaacgc accctcgacg atctacccga gtcccagcgc gctcgcgcca
      301 tcgccgtcgg caaccccgtg cggcgcgcta tccgcgccgg gaatgcggcc gcaggccgca
      361 ggtggctcgg cttcgcggac gacctgccga tcgtgctcgc gctcggcggt agccagggcg
      421 cgcggcaggt gaacgacctg atcggcgcta tccttccggc gctggccggc aaggctcgcg
      481 tcgcccacca gtgcggcccg ggcaaccccg tatgcaggcc ggcggacggc gactacaagg
      541 gcttcgagtt cgtctccggt gagctacccg acctctatgc cgcggccgac gtgatagtcg
      601 gcagggccgg cgccggtacg ctctgggaag gcgcggcctc gggcaagccg atggtcttca
      661 taccgctcgg ttcgggtagc cgcggcgacc aggtcgagaa cgcgcgcctg ctcgcgtcgc
      721 gcggcggggc cgtctgcctg gcgggcgacg aggcgacgcc cgaggccctg ctcgccgcgc
      781 tgacgccact cctgtccaat ccggaggcca ggtcgacgat ggccgccgcc gccggcgaga
      841 ccgccaggcc ggatgccgcg gccgccatag ccgggatgat actcgatcga gtactgaaag
      901 gaagcacgag atgaacgttc tagactacgt attgctggcc gtcatcgcca tcgccgcgct
      961 tcgttgctgg ttcaggggca tcgtcggaga ggtactgtcg atggcggctg tcgtgggcgg
     1021 cctcctagcc ggtattttct tcttccgacc agtcggcgcc tggatatcga acctgaagcc
     1081 gctcggcggg ttcgaggtcg tcgtcggctt cgtggccagc ttcgccgtcg tattcatcgt
     1141 cgtcaagatc gtcgagaaat cgctccgtag cgtactcgag aacctgaacc tcgacatact
     1201 cgacagcgtg ctcggcctta tcttcggcgt cgtcgaaggc gtgatcatct cggccatcgt
     1261 cgttatgatt ctccattacc aaccggtctt cgacgtcgaa gccctgctcg acggaagctg
     1321 gatcgccagg ctcctcctcc cgctcgtcgt cgaggcgcta cccgccgccc gctcgtcgat
     1381 ctccggctga gccctatgtt cgagaacatc ctgtggcagc ctaacgcgac cgaggccctc
     1441 aaggccgagc tggaccgggg agccctacca ggctcgctcc tgttcgaggg cccgcccctg
     1501 tcggcgaagc tcagctccgc cctcgagctg tccagggctc tgtcctgcga gcgcgatgcc
     1561 gcctggaact gcccctgcca gcagtgctca cggcacagga cgctatcgca ccaggatacg
     1621 ctgttgctcg gccccaagtc cttcagggag gaactcgcca taggcgcggc catgatggag
     1681 cgcgcccccg gctcgggctc gaggttcttc tttacccgtg cggtcaagaa gctgacgagg
     1741 cgattcgacc aggagctata cgccggcgag gaagcgaggc tctccaaggc cctgccgctg
     1801 gtgcgttccg ctctcgaggg cgtcgacgcc tgcgcgccgg cttccccgac gtgccgctcg
     1861 agcgccctat ccgatgacga ggccgtcaag gaagcgggta agctgctcgg cgtctgcgcc
     1921 aagctcgagg acctgctccc ggcagccacg ccggtttttc aggttcgagc catggaattc
     1981 tgggccaggc tcgcgcccta cggcaggcgc aagaccatca taatcgagca cgcggaccgc
     2041 atgctcgacg cctcaaggaa cgcgctgctc aaaatactcg aggagccgcc cgagcacgcc
     2101 gtgttcgtcc tgacctcgag ccgtaaggcg gcgctgatac cgaccgtact ctcgagggtc
     2161 agaccatatc gcttcgccca gcggacgggc gacgaggccc gagccgtgat cgagcgcatc
     2221 ttcaaggacg acgcggccgg cgccccgacg cttggcgagt acgtctcccg ctacaggccg
     2281 tcgtcgaccg gcgcagtgga cggcctggcc agggacttgg cggcggccat agttgcggcc
     2341 gccgagcgga ggcgcggccc cttcaccgag gaagcgctat cgtcgctcgc ctcgtctggc
     2401 gggccggtcc aggcggcgat cgccgcggcc cagtcagcca ccgccaactt cgggtcggcc
     2461 gacgaggctt tcgcgtgggc cttccccgcc ctgatggact cggtcggctc gatcttcgcg
     2521 gctatgctcc ggctaccagg caccgggctc gagacccagc gcgccgccga gctgtacgcg
     2581 tccttggcgc gagacgcgat gatccgctac tcgtcataca acctccagcc gacggccctc
     2641 gtcgagcgcc tggccgacgc cttcgccgac ggttgccagg acggccgccc atgaggaaat
     2701 tcctcgagcg ggcgatggag aaggtctccc gcatgaacga ggagtccatc cgcgggctcc
     2761 tgcgcgtgct ggccgacgag aacgagcggc tcgacgcggt gctcgactcg atgctcgacg
     2821 gcatcgtcgt ctgcgacgag gaccacgcgc ccatcctggt caacaaggcc gccgagcgac
     2881 tcctgccgat ggcgcaggcc gagctgttcg acctgccgct ctgggagagc gtgcgggacg
     2941 atgagctcgc cggtttcttc gacgccgcgc tgaacggcga ggagtcggtc atcgacaggg
     3001 aattcgcgct cgacgccaag gccggcacgc gcatcatatc cgtgtcggtt accccgctcg
     3061 tccgggacaa gcgtatccgc ggcaccttga tccacgtcga ggagacgacc gacaagcggc
     3121 gcagggaggc caggctacgc agggccgaga acctggcgtc gctgacgacc ctggccgccg
     3181 gcgtcgcgca cgagatcaag aacccgctcg gctccatcag catccacgtc cagctcatgc
     3241 gcaaggctct caagggcctg gccgacgagc cgccgatagg ccgctacctc gacgtcgtca
     3301 ccgaggagat cgaccgcctc aacaagatag tcgtcgactt cctgttcgcc gtccggccca
     3361 tggacatagc ccccatcaac gacgacctga acgcgctcct gcgggagctt gccgagttca
     3421 tgcgcgtcga agttgaaagc gccggcatcg agctgtcggt cgactacggg aaggacgtac
     3481 cgcccttccc gttcgaccgg cgctacctca agcaggccct gctcaacctg gtcaaaaacg
     3541 cggccgcagc catgcccgac ggcggatcgc tccggctgac gagcgaagcc tcggccgagg
     3601 aagccaggat aaccgtctca gatacgggcg tcggcatcac cgaggatcgc ctcggaaaga
     3661 tcttcgaacc gtactatacg accaaagaga acgggaccgg cctcggcctg accctgacct
     3721 ataagatagt caaggagcac ggcggcgaca tcaccgtcac ctcgaaggcg ggccagggct
     3781 cgaccttcgt ccttgcgcta ccgataccgc agcgagaccg ccgcatgatc accggggcct
     3841 gccccgacgg cgaggcttgc gagcacgagc cgctacccga tacggaggaa ggatgaggtt
     3901 taccgtactc gtcgtagacg acgagaagaa catacgcgag ggtctcggcg agtacctgcg
     3961 cctggacggc tacgacgtcg agcttgccgc cgacggcagg gagggcgtgg cgctggcgga
     4021 ccgcggcggc gtcgacctcg tcatcaccga cctgcgcatg cccgccctct ccgggggcga
     4081 actgctgagg cacgtcgtat cgcgctaccc ctcggtcccg gtcatcgtgt tgaccggcca
     4141 cggcaccgtc gaggacgccg tcgacgcgat gcgctccggc gcctacgact tcatcacgaa
     4201 gccggtcaac ctcgatcacc tgtcgatcct ggtcaagcga gcccttgagc ggcgtgagct
     4261 gtccaggcgg aacgaggagc tgctcgagga gatagagacg cagcggcgca cctcgagcat
     4321 catcggcagg agccccgcga tcagccgcat catggagctg gtgcgccggg tggctcctac
     4381 caaggcgagc gtgctgataa ccggcgagtc cggcgtcggc aaggagctgg tcgccgacgc
     4441 gatccacaac ctgtcgccgc gagccgacgg gccgttcatc aaggtgcact gcgcggctct
     4501 cgccgagtcc ctgctggaga gcgagctgtt cgggcacgag aagggttcgt tcaccggcgc
     4561 ccaggccagg aagaagggcc gcttcgagct cgccaacgaa ggcacgctct tcctcgatga
     4621 gattggcgag atcaaccaga acgtgcagat caagatactg cgggtgctgc aggacaagcg
     4681 cttcgagcgc gtcggcggcg aggag
//
LOCUS       DAOPHV010000150         4680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_24630_length_4680_cov_3.924324, whole genome shotgun sequence.
ACCESSION   DAOPHV010000150 DAOPHV010000000
VERSION     DAOPHV010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24630_length_4680_cov_3.924324"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PLQ29_05240"
     CDS             complement(<1..524)
                     /locus_tag="PLQ29_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPG86085.1"
                     /translation="MLEHIVRTKPAIQAAVKERLAAYAAEYAAVSGDFGASTAARLDE
                     FSSRGKMLRGCLVPFGYELAAGREPAGDDGRATILAGAAMELFQSGLLVHDDIMDRDR
                     IRRGGPTVHAAYEAELERGRYADPGHNGQSLGICSGDVAFFAAFRILSELPVGAEAAR
                     RVVTLLKGANEYSD"
     regulatory      620..701
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            720..1994
                     /locus_tag="PLQ29_05245"
     CDS             720..1994
                     /locus_tag="PLQ29_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPG86086.1"
                     /translation="MALSLAEIIILCLLADWAFKAFKIPGLIGMLAAGAVLGPAVLDL
                     IDPALLSIGADLRMIALIVILLRAGFELSRNTLHRVGKQALLLAFLPALCEALAVTAL
                     GPRLLGLSIMESAILGCVLGAVSPAVVVPMMVRFNQERRGVAKGIPTLVLAGASIDDV
                     TVIVAYSVLVGIYTGGRVNVAWKILGIPLSIISGIAVGLALGLALYLLFERFNPRATK
                     RALVVIGASILLVQAGDRLEALDVPFAALIAVMAIGFVILEKKESMAHELSAKLGKIW
                     VFAEIILFSMVGAQVNFRAAADAGLGGVALILIALVSRSIGTLLCTAGAGLTTREKLF
                     VVISYLPKATVQAAIGSAPLAAMAAAGMPTAPGETILAVAVMSIALTAPIGAWAIAWA
                     GRNLLAVDEQGSGDSDRVAAYESDALVDDEDR"
     gene            complement(1991..2587)
                     /locus_tag="PLQ29_05250"
     CDS             complement(1991..2587)
                     /locus_tag="PLQ29_05250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPG86087.1"
                     /translation="MSVIKILIGSTRPHRFGPAFASWIASEATAYTEANPASPRFELV
                     DLADLGLPLLDEAIPASMRKYEHDHTRRWSKVVDEADGFLFLVPEYNHGISGALKNAI
                     DYLFHEWGHKPAAFASYGGGAGGARAVEQLREICGELKVYDLRESVLVPNYWNQLDSS
                     GSFVPDEGQKKAAQAMLHQLAYWTDAMKAPREALAAKG"
     gene            complement(2685..>3368)
                     /locus_tag="PLQ29_05255"
     CDS             complement(2685..>3368)
                     /locus_tag="PLQ29_05255"
                     /inference="COORDINATES: protein motif:HMM:NF020672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HPG86088.1"
                     /translation="EEAGLASAGPGGTTAPTGTTAPTGTTAPTGTTTLFDPGRLSAFG
                     KAAAAKAGEPGGLQRAMAESLFASVFGAGKAAPGRAQAVEAAMASLEASGAVVRNGSA
                     WSTPGAEKRLTPADSALLGRLEAAGKAGLEPGKDCPQTDARGLKALCSLGLAMPLDGG
                     IFFSKAVYDACMVAILRGLGPGCRFSVPEAKARSGLSRKYILPLLNRMESQGLVKRSG
                     DERVVLKTP"
     assembly_gap    3369..3468
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3469..4666)
                     /locus_tag="PLQ29_05260"
     CDS             complement(<3469..4666)
                     /locus_tag="PLQ29_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPG86089.1"
                     /translation="MTTDLGFAWYRGGDGLDIGVVDVPGHERYLRNMVAGAWGLDLVV
                     LVVAADDGWMPQTGLHAAIVASMAEAAVVIAVTKADAVAPGRAATVALEAEDRATALF
                     GVRPPSVAVSSVSGDGITELKSAIDSRLASLPPLEPMAARLFVDRSFSPRGGGVVVTG
                     SLSGGPVGVGDEVLRWPSGDRLRVRGIQSYRESVSSAEPNCRAALSVAAPKGGFARGD
                     LLAAPGAAVLSGDEFIVRLLPLPGSGSLSAVDAKGKPALRPGVMVEFAVGSARRDAEL
                     WPIGGTGFARLTLSEPVACPLGAPFALLRLGGAELIGRGVAVRSGKTKVDDRKALLAA
                     LPRAAAAADAMTDAGWGIRGAVALALAIETLVRGWAAAPVGFPARAAEEAGLASAGPG
                     GTTAPTG"
BASE COUNT          715 a         1583 c         1558 g          724 t
ORIGIN      
        1 atatccgaat actcgttggc gcccttcagc agggtgacga cgcgccgcgc cgcctccgcg
       61 ccgacgggca gctccgagag tatcctgaac gcggcgaaga acgccacgtc tcccgagcag
      121 atgccgagcg actggccgtt atggcccggg tcggcgtagc ggccccgctc gagctcggcc
      181 tcgtaggcag catgcacggt cggcccgccg cggcggatcc ggtcccggtc catgatgtcg
      241 tcatggacga gcagaccgga ctgaaacagc tccatggcgg cgccggcgag tatcgtcgcg
      301 cgcccgtcgt cgccggccgg ctcccggccg gcggccagct cgtagccgaa gggcacgagg
      361 caaccgcgca gcatcttgcc gcgcgaggag aattcgtcga gcctggcggc cgtcgacgcg
      421 ccgaagtccc ctgagactgc ggcgtactcg gccgcatagg cggccaggcg ttccttgacg
      481 gcggcctgga tagcgggctt cgtcctgact atatgctcga gcactggact ctcctcggtt
      541 cgccagtcta gccccgcgct tcggcggggt caagcgagcc cgtgcccgat attgacgaac
      601 gattacggca tcgttaccgt gtgcgcgtgg cgatggagtt cgccctggcc ctcggtcaga
      661 accgtacgcc gaccggcggt accgatgact cctaccgaac ccccatcgat ggagtatcca
      721 tggccctaag cctcgctgaa atcatcatcc tgtgcctgct cgccgattgg gcgttcaagg
      781 ccttcaagat ccccggcctg atcggcatgc tcgccgccgg cgccgtgctc ggaccagccg
      841 tcctggatct gatcgatcca gcgctcctat cgatcggcgc cgacctcagg atgatagcgc
      901 tgatcgtcat cctcctgcga gccggcttcg agctgtccag gaataccctg caccgcgtcg
      961 ggaagcaggc cttgctcctg gcgttcctgc ccgccctgtg cgaggccctg gccgtcaccg
     1021 cgctcgggcc gcgcctgctc gggctgtcga tcatggagtc ggccatactc ggctgcgtgc
     1081 tgggggccgt atcaccggcc gtcgtcgtac cgatgatggt ccgcttcaac caggaacgcc
     1141 gcggcgtggc caagggtatc ccgaccctcg tactggccgg ggcgagcatc gacgacgtga
     1201 cggtcatcgt cgcgtatagc gtcctcgtcg gcatctatac cggaggcagg gtcaacgttg
     1261 cctggaagat cctcggcata ccgctttcga tcatctccgg tatcgccgtc ggattggccc
     1321 tcggcctcgc gttatacctg ctcttcgaac gcttcaaccc gcgggcgacg aagcgcgccc
     1381 tcgtcgtgat cggcgcgtcc atcctgctcg tccaggccgg cgaccgactg gaggcgctcg
     1441 acgtgccctt cgccgcattg atcgccgtga tggccatagg cttcgtcatc ctggagaaga
     1501 aggagagcat ggcccacgag ctgtcggcca agctcggcaa gatatgggtc ttcgccgaga
     1561 tcatcctgtt ctcgatggtc ggcgcccagg tgaacttcag ggccgccgcc gacgccggcc
     1621 tcggcggcgt cgccctcatc ctgatagccc tcgtgtccag gtccatcgga accctgctat
     1681 gcacggcggg ggccggcctg acgacgcgcg agaagctatt cgtcgtcatc agctacctgc
     1741 cgaaagccac ggtccaggcc gccatcggtt ctgccccgct cgccgcgatg gcggccgctg
     1801 gcatgccgac ggcgccgggc gagaccatac tcgccgtcgc cgtcatgagc atcgcgctga
     1861 cggccccgat cggggcctgg gcgatagcct gggccggccg aaacctgctg gccgtggacg
     1921 agcagggctc aggcgactcg gaccgcgtcg ccgcctacga gagcgacgcc ctcgtcgacg
     1981 acgaggaccg ttagcccttc gcggcgagcg cctccctcgg ggccttcatc gcgtcggtcc
     2041 agtaagccag ctggtggagc atggcctggg cggccttctt ctggccctcg tccggtacga
     2101 aggagccgct cgagtcgagc tggttccaat aattgggtac gagcaccgac tcgcgcaggt
     2161 cgtagacctt gagctcgccg cagatttctc gcaattgctc cacggctctg gcgccgccgg
     2221 cacctccgcc gtagctggcg aacgccgcgg gcttgtggcc ccactcgtgg aacaggtagt
     2281 cgatggcgtt cttaagcgct ccgctaatgc catggttgta ctccgggacc aggaacagga
     2341 agccgtcggc ctcgtcgacg accttggacc agcgtctggt gtgatcgtgc tcgtacttgc
     2401 gcatgctcgc ggggatggct tcgtcgagca agggaaggcc gaggtcggcc aggtcgacga
     2461 gctcgaagcg cggggaggcg ggattcgcct cggtgtaggc ggtcgcctcg gaggcgatcc
     2521 acgaggcgaa ggcaggcccg aaacgatgcg gacgggttga acctatcagt atcttgatga
     2581 cgctcatgct gactcctcca tatggctgat tgatgtctta tgtaatatag taaaaataat
     2641 atgtaaaaag gcaagcgaac tccgggctaa ggcgcccaag tcgatcaggg cgtcttgagt
     2701 acgacccgct cgtcgcccga gcgcttgacc aggccctgcg actccatgcg gttgagcagg
     2761 ggcaggatgt acttccgcga gaggcccgag cgcgccttgg cctccggcac cgagaaacgg
     2821 cagcccgggc ccaggccccg gagtatggcg accatgcagg cgtcgtagac ggccttggag
     2881 aagaagattc ccccgtcgag gggcatggcc aggccgagcg agcagagcgc cttgagtccg
     2941 cgtgcgtcgg tctggggaca gtccttgccc ggctcgagcc ccgccttgcc cgccgcctcg
     3001 agacggccaa gcaaggcgga gtcagccggc gtgagacgct tctcggcccc gggcgtcgac
     3061 caagccgagc cgttccgtac gacggcgccg ctcgcctcga gcgaggccat cgcggcctcg
     3121 acggcttgag cgcgtcccgg agccgccttg ccggcgccga ataccgaggc gaatagcgac
     3181 tccgccatgg cccgctggag ccccccgggc tcgccggcct tcgcggccgc ggctttgccg
     3241 aaggccgaga gtcggccggg gtcgaacagg gtggtcgtac cagtaggcgc ggtcgtgcca
     3301 gtaggcgcgg tcgtgccagt aggcgcggtc gtgccgcccg ggccggcgga ggccagccca
     3361 gcctcctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg ccagtaggcg
     3481 cggtcgtgcc gcccgggccg gcggaggcca gcccagcctc ctcagccgct ctggctggaa
     3541 atcctaccgg agcggccgcc cagccgcgta ctagcgtctc gatagccagg gctagggcca
     3601 cggcgccgcg gatgccccag ccggcgtcgg tcatcgcgtc tgccgcggcg gcggcgcgcg
     3661 gaagcgcggc caaaagcgcc ttcctatcgt cgaccttggt cttcccggag cgaacagcca
     3721 cgccgcggcc tatgagctcg gcgcccccga gcctcaggag cgcgaacggc gcgccgaggg
     3781 ggcaggcgac cggctccgac agcgtaagcc gggcgaagcc cgtgccgcct atgggccata
     3841 gctccgcgtc gcgcctggcg ctaccgacgg cgaactcgac catcacgccg gggcgcaggg
     3901 ctggcttacc cttggcgtcg accgccgata gcgagcccga gccgggtagc ggaaggagcc
     3961 ggacaatgaa ttcgtctccc gatagcacgg cggcccctgg agcggcgagg aggtctccgc
     4021 gggcgaagcc cccctttggc gcggcgacgg atagcgccgc cctgcaatta ggctctgcgc
     4081 tggacacgct ctcgcggtag ctctggatgc cgcgaaccct gaggcgatcg ccgctcggcc
     4141 accgtagtac ctcgtcgccg acgccgacgg ggccgcccga tagcgagccg gtgaccacga
     4201 cgcctccgcc gcgagggctg aaagaccggt cgacgaagag cctggcggcc atcggctcaa
     4261 gcgggggtag ggaggccagg cggctgtcga tagccgactt gagttccgta ataccatcgc
     4321 cggagacgct ggacaccgcg acggacggcg ggcggacgcc gaacagagcc gtcgcccgat
     4381 cctcggcctc gagcgcgacc gtggccgctc gtcccggcgc cacggcatcc gccttggtca
     4441 cggcgatgac gacggccgcc tcggccattg aggcgacgat ggccgcgtgg aggccggtct
     4501 gaggcatcca gccgtcgtcg gctgcgacga cgagcacgac gaggtccaga ccccaggcgc
     4561 cggcgaccat gttgcgcagg tagcgctcgt ggccgggaac gtcgacgacg ccgatgtcca
     4621 ggccgtcgcc accccggtac caggcgaagc cgaggtcggt ggtcatgccg cgggccttct
//
LOCUS       DAOPHV010000151         4653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_24826_length_4653_cov_3.535233, whole genome shotgun sequence.
ACCESSION   DAOPHV010000151 DAOPHV010000000
VERSION     DAOPHV010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24826_length_4653_cov_3.535233"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLQ29_05265"
     CDS             complement(<1..181)
                     /locus_tag="PLQ29_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86090.1"
                     /translation="MERAGRGSLTRGGPRWTALAIAAGVSATLGVAAIVAIALSLRPG
                     SDRSFGALLARIDASP"
     gene            complement(154..579)
                     /gene="nusB"
                     /locus_tag="PLQ29_05270"
     CDS             complement(154..579)
                     /gene="nusB"
                     /locus_tag="PLQ29_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPG86091.1"
                     /translation="MASRRKARILAFQAIYAWDASSVGYDELLSFSWLGEDSLASSDE
                     ELKAFAGLIVSGTIENVEAVDAAIKRHLEHWAFERLKRVDLAILRVSVYSLVYQRDIP
                     AQITIDEAIEIAKEFGSEDSYRFINGVLDAVWKERGAEA"
     gene            complement(579..1544)
                     /locus_tag="PLQ29_05275"
     CDS             complement(579..1544)
                     /locus_tag="PLQ29_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPG86092.1"
                     /translation="MKGTSLGAPLATVKLIKTDIVADKAFKDDVAKLEKSLGKTLSAD
                     ERKAYLDDVINDLLFYQMCERDGVKVSDGEIDSYIAKLRSQRPAGESDQQFAAFLASQ
                     GIPFSELKDYYRKQVLIQRWLMTARSSELSSIKPISGDEIVSTYELYKARLVRPDTVK
                     VAFLFYQYRDSSAAERAKGAEVMKGLSARLAKGESFDVLRLTAKDGGYAANQDPVYFE
                     RSDVFLSRFGKAFYDTVFALSDGENSAAFENEAGWWIVRRLEYLPQKQLELSDPYRLG
                     QAGTVQDYIGQLLAQQRESEFLQKTFAELFVKLRAQAEIKILGTP"
     gene            complement(1707..2519)
                     /locus_tag="PLQ29_05280"
     CDS             complement(1707..2519)
                     /locus_tag="PLQ29_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPG86093.1"
                     /translation="MKDYYAILGIHQEASLKEIKSAFRKRAKARHPDIVGDDDEAMRL
                     SIEAYKVLSDPASRREYDRGRLRIVKGEAEGSFDYRRWLMERLDQNEYVAKLVFYDLL
                     HDREDEALQLYERIRDSDDGRLERFFERPEAMDAEYCIAEEYASRGRFVDAFRVMLKL
                     IAMEQRSSGFGYFYEVVLARFRRLVLEDLYRVLEPEAYLETLGEALELRSSAENDAQF
                     LRRRAELLLRLGRPSEAKSAMARAWALAPRMPGLKNLARKLSSGMAKPASLE"
     gene            complement(2530..3018)
                     /locus_tag="PLQ29_05285"
     CDS             complement(2530..3018)
                     /locus_tag="PLQ29_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPG86094.1"
                     /translation="MPSRPLSLSDLFPRDRICVDLASADKEALFVELVDLIGSTGSTH
                     FDREDALACVLERESRMSTGIRPGVALPHGKTDGVDRFFGAIGVSRLGIDYDAPGGEP
                     VRIAFMLISPRSMASDHLAILKQLAGLLDAPGFVSGMIAASDPSEAHRIIERYEAQSI
                     KV"
     gene            complement(3026..3364)
                     /gene="secG"
                     /locus_tag="PLQ29_05290"
     CDS             complement(3026..3364)
                     /gene="secG"
                     /locus_tag="PLQ29_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPG86095.1"
                     /translation="MGVFGIVLLVLFAIVCLLLIFMVVIQDQEGEGLGGLFSGAGNAA
                     FGSRSSSVVVRFTYILGGLFFVIAFGLAVLNKGSVGNVEDAVRAQGGQATEWWSQESA
                     PATETAPAAE"
     gene            complement(3548..4300)
                     /gene="tpiA"
                     /locus_tag="PLQ29_05295"
     CDS             complement(3548..4300)
                     /gene="tpiA"
                     /locus_tag="PLQ29_05295"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPG86096.1"
                     /translation="MRNYYIAGNWKMHKTVAESVALAKALVAELGSCGERVMIAPAFT
                     ALSAVSEAIKGSRIVLGAQNMGPETQGAHTGEVSPWMLKDLGVRAVILGHSERRHSYG
                     ESDALVNKKVLLALAEGLEPILCVGETLEEREAGRLEAVVGTQVREGLKGVRAEDLAA
                     VTIAYEPVWAIGTGKTATPDDADAVHAYIRGVIAGLYGEAAAKAMCIQYGGSVKPENA
                     AELMAKPNIDGALVGGASLKAENFGPIANFRS"
     gene            complement(4333..>4653)
                     /gene="pgk"
                     /locus_tag="PLQ29_05300"
     CDS             complement(4333..>4653)
                     /gene="pgk"
                     /locus_tag="PLQ29_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPG86097.1"
                     /translation="GLMGLDVGPKTLVAYGEAIFQAKSVVWNGPVGVFEFDAFAGGTE
                     AIARMVADATGRGVVTVVGGGDSVAAVNKFGLADRMSHVSTGGGASLELLEGKKLPGI
                     EVCR"
BASE COUNT          840 a         1500 c         1436 g          877 t
ORIGIN      
        1 cgggcgacgc gtctatcctg gccaacaagg cgccgaagga tctatcggag cctggccgta
       61 gcgatagcgc gatcgcgacg atcgccgcga cgccgagggt ggcggatacc cccgccgcga
      121 tcgcgagcgc ggtccatcgc gggccgcccc gcgtcaggct tccgcgcccc gctctttcca
      181 tacggcatcc agcacgccgt ttatgaagcg gtacgaatcc tcggagccga attccttcgc
      241 gatctcgatc gcctcgtcaa tcgttatctg ggccggtatg tcccgttggt aaacgagcga
      301 atagaccgac acgcgcagga tggctagatc gaccctcttg agccgctcga aggcccaatg
      361 ctcgaggtgc cgcttgatcg cggcgtcgac ggcttcgaca ttctctatcg tacctgagac
      421 gatgagcccg gcgaaagcct taagctcctc gtcgctactg gccaggctat cctcgccgag
      481 ccacgagaag gacaggagct cgtcgtagcc gacggaggag gcatcccaag cgtagatggc
      541 ttggaacgcg agtatcctcg ccttacgcct cgacgccatc agggagtccc caggatcttg
      601 atctcggcct gggcgcgtag cttgacgaac agctcggcga aggttttttg taggaattcg
      661 ctctcgcgct gctgcgccag gagctggccg atatagtcct gcacggtgcc cgcctgtccg
      721 aggcggtatg ggtccgacag ctcgagttgc ttctgaggca ggtactcgag ccgcctgacg
      781 atccaccagc cggcctcgtt ctcgaaggcg gccgagttct ctccgtccga gagggcgaac
      841 acggtatcgt agaaggcctt gccgaacctc gacaagaaca cgtcgctacg ctcgaaatag
      901 accggatcct ggttggcggc gtaaccgcca tccttggccg tcagcctcaa gacgtcgaac
      961 gactcgccct tcgctaggcg agccgacagg cccttcatga cctcggcgcc cttggcgcgt
     1021 tcagcggcgc tcgaatcgcg gtactggtag aacaggaacg cgaccttgac cgtgtccggc
     1081 ctgacgaggc gggccttgta cagctcatag gtcgatacga tctcgtcgcc ggatatcggc
     1141 ttgatcgacg atagctcgga tgagcgagcc gtcataagcc agcgctggat gagtacctgc
     1201 ttgcggtaat agtccttcag ctcgctaaag ggtatgccct gggaggcgag gaaggccgcg
     1261 aactgctggt cactctctcc ggctggccgc tgggagcgta gcttggcgat ataggagtcg
     1321 atctccccgt ccgagacctt gacgccgtcg cgctcgcaca tctggtagaa caggaggtcg
     1381 ttgataacgt cgtccaggta ggccttgcgc tcgtcggcgc tcagggtctt gcctaggctc
     1441 ttctccagct tggctacgtc gtctttgaag gccttgtcgg caacgatatc ggtcttgatc
     1501 agcttgacgg tggcgagcgg ggcgccgagg ctcgtgccct tcatgggttg agcgatcgaa
     1561 ggaacggccg caaggcacga aagggcgatc gcgatcagtc tagtacgcat cgtacactcc
     1621 aggacacatc gatttgtgcg ccggttaaac gccgacgacg catgataaca aacgggagac
     1681 gcctcaggtt aatggaaacc gcgagcctat tcaaggctcg ccggcttcgc catgccggac
     1741 gacaacttcc tggccaggtt tttgaggccg ggcatcctcg gagccagagc ccaggccctg
     1801 gccatggccg acttggcctc ggaaggtcgg ccgaggcgta gcagcagctc ggctcgtctt
     1861 cgcaggaatt gggcgtcatt ctcggcggag ctccgtagct cgagggcctc cccgagcgtc
     1921 tccaggtagg cttcaggctc cagcacgcga tagaggtcct cgaggaccag ccgcctgaac
     1981 cgtgccagca ccacctcgta gaagtatccg aagccggacg accgctgttc catggcgatg
     2041 agcttcagca tgacgcggaa cgcgtcgacg aagcgccctc gggaggcata ttcctcggcg
     2101 atacagtact cggcgtccat cgcctccggc cgctcgaaga aacgctcgag gcgcccgtcg
     2161 tcggaatcgc ggatcctctc gtacagctgg agcgcctcat cctccctgtc atggagcaaa
     2221 tcatagaaga cgagcttggc cacatactcg ttctggtcga ggcgttccat cagccagcgc
     2281 cggtaatcga aggagccctc ggcctcgccc ttgactatac gcagtcgccc cctatcgtac
     2341 tcccgcctcg aggcaggatc ggataggact ttgtacgcct cgatcgatag gcgcatcgct
     2401 tcgtcgtcgt ccccgacgat gtctggatgg cgagccttgg cccgtttcct gaacgcgctc
     2461 ttgatctctt tcagggaggc ttcttgatgg atcccgagga tggcgtagta atctttcacg
     2521 ttcgtttctt taaaccttta tgctctgcgc ttcatagcgc tcaatgatcc tatgggcttc
     2581 cgatggatcc gaggcggcga tcatcccgga gacgaagccc ggggcgtcga gcagccccgc
     2641 gagctgtttc aggatggcca aatgatcgct cgccatcgac cgcggcgata tgagcatgaa
     2701 ggcaatccgt acgggctcgc cgcccggagc gtcataatcg atcccgagcc ttgagacgcc
     2761 tatcgccccg aagaaacggt cgacgccatc ggtcttgcca tggggtagcg cgacgccggg
     2821 gcgtataccg gtggacatgc ggctctcccg ctccagtacg caggcgagcg cgtcctctcg
     2881 gtcgaaatgc gtcgagcccg tcgatcctat cagatcgacg agctcgacga acaaggcttc
     2941 cttgtcggcg ctcgcgaggt cgacgcatat cctgtcccgg gggaacaggt cgctgagtga
     3001 gagcggcctc gaaggcacgt gcgtcctatt cggcggcggg agcggtctcg gtagcggggg
     3061 cggattcttg gctccaccat tccgttgcct ggccgccctg ggcgcgcacg gcgtcctcga
     3121 cgttgccgac ggagcccttg ttcaggacgg cgaggccgaa cgctataacg aagaatagtc
     3181 cgccaagtat gtaggtgaag cggacgacca cgctcgacga tcgcgaaccg aacgccgcgt
     3241 tgccggctcc ggaaaagagc ccgccgaggc cctcgccttc ctgatcctgg atgacgacca
     3301 taaatatgag caacaggcag acgatcgcga agagaacgag aagcactatg ccgaaaacac
     3361 ccatctgatt gactccgatt tgtattgggt ctggcttacg cgacctatcc gacgcgccgc
     3421 gccggattaa ttcggcgcga tgcgccgggt agacgccgcc tcgcgcgata ctataccaga
     3481 tcgaagcggc cgcgcaaggg gcgtcatgac gaaaagccgc gggcgccagg cccgcggctt
     3541 cgcacgttca cgacctgaaa tttgctatcg ggccgaagtt ctcggccttg agcgaggcgc
     3601 ccccgacgag cgcgccatcg atgttcggct tggccatcag ctcggcggcg ttctccggct
     3661 tgaccgagcc gccgtactgg atgcacatgg ccttggctgc ggcctcgccg tacaaaccgg
     3721 ctatcacgcc gcgaatatac gcgtgaaccg cgtcggcatc gtcgggcgtg gccgtcttgc
     3781 cggtgccgat ggcccagacc ggctcgtagg cgatggtgac cgcggccagg tcttcggctc
     3841 tcacgccctt gagaccctcg cggacctgcg tgccgaccac ggcctcgagc cttccggcct
     3901 cgcgctcctc gagcgtctct ccgacgcaga gtatcggctc gagcccctcg gctagcgcca
     3961 gcaggacctt cttgttaacc agagcgtcgc tttcgccgta cgagtgcctg cgttccgagt
     4021 gaccgagtat gacggctcgg accccgaggt ccttgagcat ccatggcgag acctcgccgg
     4081 tatgggcgcc ctgcgtctcc gggcccatat tctgggctcc gagcacgatc cggcttccct
     4141 tgatggcctc cgacacggcg gatagagccg tgaaggccgg cgctatcatc acgcgttcgc
     4201 cgcacgaacc tagctcagcg acgagcgcct tggccagggc cacggactcg gcaacggtct
     4261 tatgcatctt ccagttgccg gcgatatagt agttcctcac gatagtactc ctgattagtg
     4321 cgtagccgcg gcttagcggc agacttcgat gcccggcagc ttcttgccct cgagcagctc
     4381 caatgacgcg cctccgccag tggatacgtg gctcatcctg tcggccaggc cgaatttatt
     4441 gacggccgcg accgagtcgc cgccgcccac gacggtgacg accccgcgcc cggtggcgtc
     4501 ggcgaccatc ctggctatcg cctcggtgcc gccggcgaaa gcgtcgaact cgaagactcc
     4561 gacggggcca ttccagacga cgctcttggc ctggaagatg gcctcgccgt aggcaacgag
     4621 ggtcttcgga ccgacgtcga ggcccatgag ccc
//
LOCUS       DAOPHV010000152         4648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_24856_length_4648_cov_4.632702, whole genome shotgun sequence.
ACCESSION   DAOPHV010000152 DAOPHV010000000
VERSION     DAOPHV010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24856_length_4648_cov_4.632702"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PLQ29_05305"
     CDS             complement(<1..464)
                     /locus_tag="PLQ29_05305"
                     /inference="COORDINATES: protein motif:HMM:NF037644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPG86098.1"
                     /translation="MWAALAEGSSALEGSGSGSPFLDAALLLGHTLGVGRDRVLASMP
                     DELGASALEAYQAFVARRSSGEPIAYIVGYKEFYGRRYTVDPRVLVPRPDTELLVEVA
                     LSLLPPVSRTRGGSARVRCHDAFTGSGCIGVTLAAERPDVEVSLSDASPAAL"
     gene            complement(493..1563)
                     /gene="prfA"
                     /locus_tag="PLQ29_05310"
     CDS             complement(493..1563)
                     /gene="prfA"
                     /locus_tag="PLQ29_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPG86099.1"
                     /translation="MNERIEAVASRLRQLEDLVSDPGLSRDPKRYKDAMRERSRLAEI
                     VEAYDGVRRIQAEIAGTKAMLHDEADPELKELARDELRGLEDGLEAAEKRVSELLVPR
                     DPLDDKNTIMEIRAGTGGDEAALFVADLYRMYTHYADEKGWKIEVMSSSETGLGGFKE
                     IILSISGDQVFGKLRWESGVHRVQRVPATEGSGRIHTSAVTVAVLPEVEETEVEIRPE
                     DLRVDVMRAGGPGGQCVNTTDSAVRLTHIPTGLVVHCQDEKSQIKNKAKAMRILRARI
                     YELEEEKKNAERAAERRGQIGSGDRSERIRTYNFPQNRVTDHRIDVTLYKLDLFMQGD
                     MDEIVQGLAIRAREDAMKAEGQ"
     gene            complement(1560..1739)
                     /locus_tag="PLQ29_05315"
     CDS             complement(1560..1739)
                     /locus_tag="PLQ29_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86100.1"
                     /translation="MKLDMNPRGNGACPLCVSHGSCRVQRKLIENLSLVGPDDGEGME
                     IVIYSCPYFKEKIAP"
     gene            2025..3299
                     /gene="serS"
                     /locus_tag="PLQ29_05320"
     CDS             2025..3299
                     /gene="serS"
                     /locus_tag="PLQ29_05320"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPG86101.1"
                     /translation="MLDFKYIKDNLEAVKKNIDDRFMKADADLVASLYDKRNELLKRL
                     EDERRARNENAAAMKGKLEQAVRERLIAEGKRLKDSIAAIEAEAERILASLDDQVRRI
                     PNMAHPDAPVGKEDKDNLEVKRVGEPTAFDFEPKDHVQLGQDLDIIDFDAGTKVSGTK
                     FYYLKNEGVFLELGLVRYALDILARKGFTPFITPDVAKTEILEGIGFNPRGAESNVYS
                     VEGEGSCLVGTAEITLGGYYSDTILPKDRLPLRMAGLSHCFRREAGSSGQFSKGLYRV
                     HQFTKLEMFVYCLPEDSDRLHEELRLIEEEIFAGLGLPFRVVDTCTGDLGAPAYRKWD
                     LEAWMPGRNGGEWGEVTSTSNCTDYQARRLNIKYKDDDGRNKYVHTLNGTAVAISRAL
                     VALLENYQQADGSVMMPAVLVPYVGFEVIRKK"
     gene            complement(3336..>4648)
                     /locus_tag="PLQ29_05325"
     CDS             complement(3336..>4648)
                     /locus_tag="PLQ29_05325"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPG86102.1"
                     /translation="PARFWAYMAAWLALLTIGTYVYDSFAVRAIGASVFIIAASVEFL
                     VAVRGVGEGIPPIIRRAAWGVVLAFIACHLVRVGLVAANANARLMDDAFVNAYTFSFT
                     LIFSIAMAGLILIIDVSHLVSLLEKKNAKLESLAMCDGLTGLRNRHALELKLEGELER
                     ASRYRVPLSVILFDIDYFKRVNDTWGHQAGDGVIKRMAELTSALVRQPDDLFRWGGEE
                     FMLVSPHTDRAGATALAEKLRAAIEAESFPEAGTVTASFGVAEWKPGEDCDEWFKRVD
                     RALYRAKNTGRNRVVGIDAGEAAPAAMVRIDWKAEWSSGNRLIDGEHRELLGLSNNLI
                     DLSLVGADATAMLEAFDALIGHIQKHFGDEERVLAELVYPGLAGHALLHAKLVSESLE
                     LRAKVEAGELDHGALFDFLVDKVVVEHLVREDSQFFEYTHRPSA"
BASE COUNT          852 a         1532 c         1476 g          788 t
ORIGIN      
        1 tcgagggcgg ccggggacgc gtccgatagc gagacctcga catcgggacg ctcggccgcc
       61 agcgtcacgc cgatgcagcc agagccggta aaggcgtcgt ggcaccgtac ccgggccgat
      121 cctccgcgcg ttcgcgatac cggcgggagt agcgacagag ctacctccac gagcagctcg
      181 gtatccgggc gcggtaccaa tacgcgcggg tcgacggtat atcgtcgtcc atagaattcc
      241 ttatacccga cgatatacgc gataggttcg ccggaggacc tgcgggccac gaaggcctgg
      301 tacgcctcga gcgccgaggc gccgagctca tccggcatgg aggcgaggac cctatcgcgc
      361 cccacaccga gtgtgtgacc gaggagtagg gcggcgtcca ggaaaggcga tcccgagccg
      421 ctcccctcga gggcgctgga tccttccgcg agcgccgccc acacggtcac ggggtcggct
      481 ccgatcgggg gatcactgcc cctccgcctt catcgcgtcc tcgcgggcgc ggatggcgag
      541 gccttgcacg atctcgtcca tgtcgccttg catgaacagg tccagcttgt acagcgtcac
      601 gtcgatacga tggtcggtga cgcggttctg cgggaagtta taggtacgga tgcgctcgga
      661 acgatcgccc gagcctatct ggccgcggcg ctcggcggcc cgctccgcgt tcttcttctc
      721 ttcctccagc tcgtagatgc gcgcccggag gatgcgcatc gccttggcct tgttcttgat
      781 ctggctcttc tcgtcctggc agtggacgac gaggccggtc ggtatatggg tcagccggac
      841 cgccgagtcg gtcgtgttga cgcactgtcc gccagggcct ccggcgcgca tcacgtcgac
      901 gcgcaggtct tcgggccgga tctcgacctc ggtctcctcg acctcgggca gcaccgcgac
      961 cgtaacggcg ctcgtatgga tgcggccgga gccctcggtg gccggtacgc gttgcacgcg
     1021 gtgcacgccc gactcccagc gaagcttgcc gaagacctgg tctccggaga tcgacaggat
     1081 gatctccttg aaaccgccga ggccggtctc cgagctggac ataacttcga tcttccagcc
     1141 cttctcgtcc gcgtagtgcg tatacatacg gtagaggtcc gccacgaaca gggcggcctc
     1201 gtcgccgccg gtaccggcgc ggatctccat aatggtgttc ttatcgtcga gcgggtcgcg
     1261 aggtacaagt agctccgaaa cacgcttctc ggccgcctcg aggccgtcct cgaggccacg
     1321 caactcatca cgggccagtt ccttgagctc ggggtccgcc tcgtcgtgca gcatggcctt
     1381 ggtgcccgct atctcggcct ggatacgcct tacgccgtca taggcctcga cgatctcggc
     1441 caggcgggag cgctcgcgca tggcgtcctt ataacgcttg gggtcgcgcg ataggcctgg
     1501 atcggagacg aggtcttcca gctgcctaag gcgggacgcg accgcttcga ttcgctcgtt
     1561 cacggcgcga tcttttcctt gaaatacggg caggagtaga tgacgatttc catgccttcg
     1621 ccgtcgtcag ggccgacgag gctcaggttc tcaatcagct tgcgctggac gcggcacgac
     1681 ccgtgcgata cgcacagggg gcaggcgccg ttgccgcggg ggttcatatc gagcttcatg
     1741 gcgctagggt acaaaaaaaa cgcttggaat acaagacgcg agccggacgg cgcgcgggac
     1801 cgttgacgcg acgacccggt tgcctacctg ccgcgccgca ggatactatg cggcatgggc
     1861 cgagtcgacg tagccggaat cacgaaacct tcctcgtcat cctcctccag tcgaggctcc
     1921 tccgccgacc ggccgggcgg ggcgagcgat agccgttcct aaggccggac gagcgcgccg
     1981 gcgcaagcca cgccaagacc gacatccaga ccaaggagaa cagcatgctc gactttaaat
     2041 atatcaagga caacctcgag gcggtcaaga agaacatcga cgaccgcttc atgaaggcgg
     2101 acgccgacct ggtcgcgtcg ctgtacgata agcgcaacga gctcctcaag cggctcgagg
     2161 acgagaggcg cgcccgcaac gagaacgccg ccgcgatgaa gggcaagctc gagcaggccg
     2221 tacgcgagcg gctgatagcc gagggcaagc gcctcaagga ctcgatagcc gcgatcgagg
     2281 ccgaagccga gcggatactc gcgagcctcg acgaccaggt gcgtaggata ccgaacatgg
     2341 cccatccgga cgccccagtc ggcaaggaag acaaggacaa cctcgaagtc aagcgggtgg
     2401 gcgagccgac ggccttcgat ttcgaaccca aggaccatgt ccagcttggt caggacctcg
     2461 atatcatcga cttcgacgcg ggcaccaagg tatcgggcac gaaattctac tacctcaaga
     2521 acgagggcgt gttcctcgag ctcgggctcg tgcgctacgc gctggacatc ctggccagga
     2581 agggcttcac gccgttcatc acgccggacg tcgccaagac cgagatactc gagggcatag
     2641 gcttcaaccc gcgcggcgcc gagtcgaacg tctactcggt cgagggcgag ggctcctgcc
     2701 tcgtcggcac cgccgagatc acgctcggcg gttactactc cgatacgata ctgcccaagg
     2761 atcggttgcc gctccgcatg gccggcctgt cccactgctt ccgtcgcgag gcgggctcct
     2821 cgggccagtt ctccaagggc ctctatcgcg tgcaccagtt caccaagctg gagatgttcg
     2881 tctactgcct gcccgaggac tccgaccgcc tgcacgagga gttgcgcctg atcgaggagg
     2941 aaatcttcgc cgggctcgga ctgcccttcc gcgtggtgga cacctgcacc ggcgacctcg
     3001 gcgcgccggc ctaccgcaag tgggacctcg aggcctggat gcccggccgc aacggcggcg
     3061 aatggggcga ggtgacgagt acctccaact gtaccgacta ccaggcccgg cggctcaata
     3121 tcaagtacaa ggacgacgat ggcaggaata aatacgtgca caccctcaac ggcacggccg
     3181 tcgccatctc gcgggccctc gtcgccctac tcgagaatta ccagcaggcc gacggcagcg
     3241 tcatgatgcc ggccgttctc gtaccgtacg tcggcttcga ggttatccgc aagaagtgag
     3301 ccgccggcct acggtctgag cggatccggc gacgctcagg ccgaaggccg atgcgtgtac
     3361 tcgaagaact ggctgtcctc tcggaccagg tgctcgacga cgaccttgtc gacgaggaag
     3421 tcgaacaggg cgccgtggtc gagttctccg gcctcgacct tggcgcgcag ctcgagcgat
     3481 tctgatacca gcttggcgtg caggagcgcg tgccctgcga gccccggata gacgagctcg
     3541 gccaagaccc gttcctcatc gccaaagtgc ttctggatat ggccgatgag ggcgtcgaac
     3601 gcctcgagca tggccgtcgc gtcggcaccg acgagcgaca ggtctatcag gttgttcgat
     3661 aggccgagca gctcgcggtg ctcgccgtcg atgagccgat tgccggacga ccactcggcc
     3721 ttccagtcga tgcgcaccat cgccgccggt gcggcctctc cggcgtcgat gcccacgacg
     3781 cgattgcgtc cggtattctt ggcccggtag agcgcccggt ccacccgctt gaaccattcg
     3841 tcgcaatcct cgccgggctt ccactccgcg acgccgaaac tggccgtcac cgtgccggcc
     3901 tccgggaagc tctcggcctc gatggcggcg cgcagcttct cggcgagcgc cgtcgcgccg
     3961 gccctatcgg tgtgcggcga cacgagcatg aattcctcgc cgccccagcg gaacaggtcg
     4021 tcaggctggc gtaccagcgc gctcgtcagt tcggccatac gcttgatcac cccgtcgccc
     4081 gcctggtgtc cccaggtatc gttgactcgc ttgaagtagt cgatatcgaa gagtatgacc
     4141 gacaaaggca ctcgatacct cgaggcgcgt tccagctctc cctcgagttt cagctccagc
     4201 gcatgccggt tccgcaggcc cgtcagcccg tcgcacatag ccaggctctc gagcttagcg
     4261 ttcttcttct caagcaggct cacgagatgg gacacgtcga tgatcaggat gagccccgcc
     4321 atcgctatcg agaagatcag ggtgaacgag aacgtatagg cgttgacgaa agcgtcatcc
     4381 ataagtctgg cgttggcgtt agccgcgacc agtccgacgc gaaccaggtg gcaggcgatg
     4441 aacgccagta cgacgcccca ggcggcgcgc cggattatag gcggtatccc ctctccgacg
     4501 ccccggacgg cgacgagaaa ctcgacgcta gccgcgatga tgaagactga agcgccgatg
     4561 gcccgtacgg cgaatgaatc gtagacgtag gtacctatgg tcaacagagc aagccatgcg
     4621 gccatgtagg cccagaaccg ggcgggcc
//
LOCUS       DAOPHV010000153         4644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_24884_length_4644_cov_4.554587, whole genome shotgun sequence.
ACCESSION   DAOPHV010000153 DAOPHV010000000
VERSION     DAOPHV010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24884_length_4644_cov_4.554587"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..689)
                     /locus_tag="PLQ29_05330"
     CDS             complement(192..689)
                     /locus_tag="PLQ29_05330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG86103.1"
                     /translation="MIRTATTDDIPRIAEIQIGGWRYAYRGLLSDHELFSERLVVRAV
                     KATAARLEAGCEFVVFDDPADGVVKGFAWHGRCRDDDAADSYEINAIYVQPEFCRGGI
                     GGSMLGFVADYARARGYGELIVWVLDGNAKGLGFYEKHGFIADGKRKLIEKWGREEIR
                     MVKRL"
     gene            complement(702..1217)
                     /locus_tag="PLQ29_05335"
     CDS             complement(702..1217)
                     /locus_tag="PLQ29_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86104.1"
                     /translation="MELRRFFSPSTVGLGTAIAAASSALSFLFAFIGLGRPYYASVFM
                     PLAMICLLALAWFMYLRDDGLARRPPASGRPSGASGAPATGAESEPWSAEPVAVGGLD
                     LDGGAAGSAAPYSVLFVPRSSGLVPRAPVAAPAGRRLSVRGACVWAAFELGAASAVLY
                     AATGLGASFFQ"
     gene            complement(1304..1801)
                     /locus_tag="PLQ29_05340"
     CDS             complement(1304..1801)
                     /locus_tag="PLQ29_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86105.1"
                     /translation="MAKRRVLLVAALVILAALAFAEPVAKDGMIIAPGTLDVNVGIGY
                     GYVWGLDIGGGAEFAIGKFMIGESLPFSYGVAARAGLGIGDTFPLSLAALGTVHFCWG
                     AIEWPDELAWLANVDSYIGLGLQILPGLYIASIGGSSYFINEHFAINVEGGLRASYLG
                     VLYKF"
     gene            complement(1838..3049)
                     /locus_tag="PLQ29_05345"
     CDS             complement(1838..3049)
                     /locus_tag="PLQ29_05345"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HPG86106.1"
                     /translation="MAYRTGFSLHRAADGAFDSWYLSGCSRRNGRLTLDPSAPPVSGE
                     SGSGGDGLPAAFVVGEATSPVLAPDFPFRSCVPSWNADVPDGSWLELSLRTRSGGRWS
                     GWKSLGPWAPNDSTVRRHSIAGQDDDEARVATDTVEPTSPADAFQTRFRLYAAVGADG
                     RPVSAAMPGLRSFSLAYSDPKPVDVEPSAAVSGDAVVIEGVPRSSQMVYPDGGKVWCS
                     PTSVSMVLGYWAGDRSPDGAAREARVRAAVAGVYDHVYGGHGNWSFNVAYAGSAPGME
                     AYVARFEGLDRLEPWLSAGVPLVLSVAWDTGAGRPITAAPVPRSSGHLTTLVGFTARG
                     DAVMHEPASPSNDSVRRVYDRRELESRWLAASGGAAYVIFPSAAGPDDRSRLFASTIG
                     RRLAGIDGMAL"
     gene            complement(3053..4237)
                     /locus_tag="PLQ29_05350"
     CDS             complement(3053..4237)
                     /locus_tag="PLQ29_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPG86107.1"
                     /translation="MSEYLRLAGIVDEAIALKAAEAIALSDAMAADPEISEREFRSSE
                     AHVAFLRGCGFEVEYPFFGIPTAYNGKVSRGSGGRVALLAEYDALPGIGHACGHNVHG
                     AMSLLAGAGLASVLEELGGELRVVGTPAEETNGAKITMAAGGAFDGLDLALMIHSDND
                     DCRVRYRCLAMDGIEFTFRGKASHAAASPWEGRNALNGAQLFFHAIDMLRQHVRPDVR
                     MHGIYVDGGEACNIVPETAVVRFYFRSSRRAQLNDILRKAYAAAKGAAMATETEVEWR
                     QFELSFDELVPNEAAEAMMEGVYDELGVPYGPVSGPEGSSDVGNVSMRCPTLQPTLSI
                     VDRPFALHTREFAAATTAPMAHDAIATGAKILARAALRTFLDPGLRDAMRACMEAGRE
                     KA"
     gene            complement(4311..>4644)
                     /locus_tag="PLQ29_05355"
     CDS             complement(4311..>4644)
                     /locus_tag="PLQ29_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HPG86108.1"
                     /translation="PRWIAAEGMLVVQTLINFFIPSGSGQAATTMPIMAPLSDLLGIS
                     RQTAVLAFQYGDGFSNLLWPTTLLPVVCSIAKIPIQKWWRFFVPFFGLLLVAQVIFIA
                     VSVMIGLA"
BASE COUNT          799 a         1558 c         1638 g          649 t
ORIGIN      
        1 tcggccgcta tcgcgtaggg taagaccgta tcgccgacgt agcgcggcga tacggcagcc
       61 gttgttcaag cggtcgagct agcgctcgcc gcttaacggc atggagcgga ccttcggccg
      121 ctatcgcgta gggtaagacc gtatcgccga cgtagcgcgg cgatacgggt actgcgacgc
      181 ggcggcggcc gttagagccg cttgaccatc cgaatctctt ccctgcccca tttttcgatg
      241 agcttgcgct tgccgtcggc gatgaagccg tgcttctcgt agaagccgag ccctttggcg
      301 ttgccgtcca ggacccacac gatcagctct ccatagcccc gcgccctggc gtagtcagcc
      361 acgaagccga gcatggagcc gcctatcccg ccgcggcaga attccggctg cacgtagatc
      421 gcgttgatct cgtacgagtc ggccgcgtcg tcgtcgcgac agcgaccgtg ccatgcgaag
      481 cccttgacga cgccgtccgc cgggtcgtcg aagacgacga attcgcagcc cgcctcgagg
      541 cgcgccgccg tcgccttgac ggcccggacg accaggcgct cggagaacag ctcgtggtcg
      601 gatagcaggc ctcggtacgc gtagcgccag ccgcctatct ggatctcggc tatcctgggt
      661 atgtcgtccg tagtcgcggt ccgtatcatg ggtgcttctt actattgaaa gaagctggcg
      721 ccgagcccgg tcgcggcgta cagaacggcc gacgcggcgc ccagctcgaa ggcggcccag
      781 acgcaagcgc cccggacgct gagacgacgg ccggcgggag cggcgaccgg ggcccggggt
      841 acgaggccac tggaacgggg aacgaacagg acggagtacg gcgcggccga gcctgcggca
      901 ccaccgtcca agtcgaggcc gccgacggct acgggttcgg ccgaccacgg ttccgactcg
      961 gccccggtgg cgggcgcgcc ggacgcgccg gacggccgcc ccgaggcggg cggcctcctg
     1021 gccagtccat cgtcccgaag gtacatgaac caggccaggg cgaggaggca gatcatggcg
     1081 agcggcatga aaactgaggc gtagtacgga cggccgaggc cgatgaacgc gaacaggaag
     1141 gacaaagccg acgaggcggc cgcgatggcg gtgccgaggc cgaccgtgct cgggctgaag
     1201 aaacgccgaa gctccatgga gcggcagtat ggtggcgcgg cgagcgcttg tcaataaaaa
     1261 cgggcgccag gaggcgcccg ttcagtctac cgtgacgcct ggatcagaac ttgtagagca
     1321 cgccgaggta cgaggcgcgc aggccgccct caacgttgat ggcgaagtgc tcgttgatga
     1381 agtagctcga gccgcctatc gaggcgatat agaggccggg gaggatctgc aggccgaggc
     1441 cgatgtagct gtcgacgttg gccagccagg ccagctcgtc gggccactcg atggcgcccc
     1501 agcagaagtg caccgtgccg agggcggcga gcgacaaggg gaaggtatcg ccgatgccga
     1561 ggccggcccg ggcagcgacg ccatagctga agggcaggct ctcgcctatc atgaacttgc
     1621 cgatggcgaa ctccgccccg ccgcctatgt cgaggcccca gacatagccg tagccgatgc
     1681 ccacgttcac gtcgagcgtg ccgggagcta tgatcatgcc gtccttggcg actggctcgg
     1741 cgaacgcgag agcggccagg atgacgagcg ccgcgaccag gagaactctt cttttagcca
     1801 tacgtacctc cgaagggatg aagtgaatgt accatcacta tagagccatc ccgtcgatcc
     1861 cggcaagccg tcggccgatg gtcgaggcga agaggcgcga gcggtcatcc gggccggcgg
     1921 ccgacgggaa gatgacgtac gccgctccgc ccgaggccgc cagccatcgc gattctagct
     1981 cgcgccggtc gtagacgcgg cggacgctat cgttcgacgg cgaggccggc tcgtgcatca
     2041 cggcgtcgcc gcgggcggta aagccgacca gcgtggtcag gtgaccggag gatctgggca
     2101 ccggcgcggc cgtgatgggg cggcccgcgc cggtatccca ggcgaccgac agcaccaacg
     2161 gcacgccggc cgacagccag ggctcgaggc ggtcgaggcc ctcgaagcgg gccacatagg
     2221 cctccatgcc cggcgccgag ccggcgtacg cgacgttgaa cgaccagttg ccgtgcccgc
     2281 cgtagacatg gtcgtagacg ccggcgaccg cggcccggac gcgggcctcc cgggcggccc
     2341 cgtcgggact gcggtcgccg gcccagtagc cgagcaccat cgatacggag gtcgggctgc
     2401 accagacctt gccgccgtcc ggatagacca tctgcgagct gcgcggcacg ccctcgatga
     2461 cgacggcgtc gcccgaaacc gcggcggagg gctcgacgtc gaccggcttg ggatctgagt
     2521 acgcgagcga gaacgaacgg agcccgggca tggcggccga gacggggcgg ccgtcggcgc
     2581 cgaccgccgc gtagaggcgg aagcgcgtct ggaaggcgtc ggcggggctc gtgggctcga
     2641 cggtgtcggt cgcgacgcgg gcctcgtcgt cgtcctgccc cgcgattgaa tggcgcctga
     2701 cggtcgagtc gttcggagcc cagggcccga gcgacttcca gccgctccaa cgcccgccgg
     2761 agcgggtacg cagcgatagc tcgagccacg agccgtcggg cacgtcggcg ttccaggacg
     2821 gcacacagga gcggaacggg aaatcgggcg ccagaacggg gctcgtcgcc tcgccgacga
     2881 cgaacgcggc cgggaggccg tcgccgcccg acccggactc gcccgagacg ggcggcgcgg
     2941 acggatcgag cgtcaggcgg ccgttccgcc tcgagcatcc ggacaggtac cacgagtcga
     3001 acgccccatc ggcggcccga tggagcgaga aaccggtacg gtacgccacg gcctacgcct
     3061 tttcccggcc cgcttccatg caggcgcgca tcgcgtcccg gaggcccggg tccaggaagg
     3121 tcctgagcgc ggcgcgggcc aggatcttgg ccccggtggc gatcgcgtca tgcgccatcg
     3181 gcgcggtcgt ggcggcggcg aattcccggg tatgcagggc gaagggccta tcgacgatcg
     3241 acaacgtcgg ctggagcgtc gggcaacgca tactgacgtt cccgacgtcc gacgagcctt
     3301 ccgggcccga gaccggaccg tagggcacgc ccagctcgtc gtagacgcct tccatcatcg
     3361 cctcggcggc ctcgttcggc acgagttcgt cgaaggacag ctcgaactgg cgccactcca
     3421 cctcggtctc cgtggccatc gcggctccct tcgccgcggc gtaggccttc cgcaggatgt
     3481 cgttcagctg ggcgcgacga gaggagcgga aatagaagcg cacgacggcg gtctccggca
     3541 cgatgttgca cgcctcgccg ccgtcgacgt agatgccgtg catacggacg tcgggccgga
     3601 cgtgctgcct gagcatgtcg atggcgtgaa agaacagctg cgccccgttg agcgcgttgc
     3661 gaccctccca gggcgaggcg gccgcgtgcg aggccttgcc acggaacgtg aactcgatgc
     3721 cgtccatcgc caggcagcgg tagcgcaccc ggcagtcgtc attgtccgaa tggatcatca
     3781 gcgcaaggtc cagcccgtcg aaggcgccgc ccgcggccat ggtgatcttg gcgccgttcg
     3841 tctcctcggc cggggtgccg acgacccgga gctcgccgcc gagttcttcc aggacggacg
     3901 ccaggccagc gccggccaag aggctcatcg cgccgtgcac attatgcccg caggcgtggc
     3961 ctatgcccgg tagggcgtcg tactcggcca ggagcgccac gcgcccgccg gagccgcggg
     4021 acaccttgcc gttataggcc gtcgggatgc cgaagaaggg gtactccacc tcgaagccgc
     4081 agccccggag gaacgcgacg tgagcctcgc tgctccggaa ctcccgctcc gatatctcag
     4141 gatccgccgc catcgcgtcg ctcagggcga tcgcctcggc ggctttcagc gctatagcct
     4201 cgtcgacgat cccggccagc ctcagatact cgctcacgtc gtctcctttc cgccgcggcg
     4261 cccctccgtc cgtgaagggc gccgtcgtca gtgtagaacg gttctggccg tcaggccagc
     4321 cctatcatga ccgagaccgc gatgaagatg acctgggcga ccaagaggag gccgaagaac
     4381 gggacgaaga agcgccacca cttctggatc gggatcttgg cgatggagca gacgaccggc
     4441 aatagcgtcg tcggccacag caggttggag aagccgtcgc catactggaa cgccagcacc
     4501 gcggtctgcc tcgagatgcc caggaggtcg gacagcgggg ccatgatcgg catggtcgtg
     4561 gcggcctggc ccgagccgga cgggatgaag aagttgatca gggtctgcac gacgagcatg
     4621 ccctcggcgg ctatccatcg cggc
//
LOCUS       DAOPHV010000154         4631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_24984_length_4631_cov_3.556818, whole genome shotgun sequence.
ACCESSION   DAOPHV010000154 DAOPHV010000000
VERSION     DAOPHV010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24984_length_4631_cov_3.556818"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLQ29_05360"
     CDS             complement(<1..204)
                     /locus_tag="PLQ29_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86109.1"
                     /translation="MRRIRTIATAGLALAVMLVAAACQAPVWENFGLLEFPDLEAYRG
                     PLGGRLGEVAFGDILLRLFYRLKR"
     gene            complement(201..869)
                     /locus_tag="PLQ29_05365"
     CDS             complement(201..869)
                     /locus_tag="PLQ29_05365"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HPG86110.1"
                     /translation="MMIQVYLSFVVPLAFLLLVVTGEGRGVMASFAFGLSAALAVYGL
                     TGFISEAVVPLPMQLAFVIPAIEEAAKVLPLVYLLLRSKKTGKYSLVRYAMAIGIGFS
                     ILENYMYLTMAADSGVSSTIVFTLLRSLTASLLHGSTTALSGFCFQLMVNRSRFSPAL
                     ALGTLLGGCAVHSLYNALLLNAEHGAWAIVLPIASFGFVLFGVNGFGIVGSPRNRRKE
                     GASA"
     gene            complement(869..1651)
                     /locus_tag="PLQ29_05370"
     CDS             complement(869..1651)
                     /locus_tag="PLQ29_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86111.1"
                     /translation="MSGASAAAPKRPLTAPSMPTWLYASLGAFLALAYAVLVAPGRFE
                     AIAGLGGPSELILFWLDFGLPIVLLAAMLIAAGPRRLEGSMALAMLAMLAAAASGSLY
                     ALSGRLEFVPEAVKQAASPTGVVVSLIWVGRAGLYPGDEALVEAPVGRRATIAAVLVA
                     LVVALALYARPDGARGYYALALLAPQTLVVAIAALRFRRLSAAWLAPLVAYWLAYLAA
                     YAAGLLPEAAAAWIPAAWRHAPSVCAVAAIAALSLSDAEARP"
     gene            complement(1648..3012)
                     /locus_tag="PLQ29_05375"
     CDS             complement(1648..3012)
                     /locus_tag="PLQ29_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86112.1"
                     /translation="MALAKPRTGGTYLLGFDFSGDLSSAYELLDRYDSPTQYKLTQLY
                     DWPLEFGLKGLLANKGKSSSSGYSLDYDFSYTPNSYRWVTDTSQEPLLKYADAVSNDA
                     DVMSHAATLRGDWSGKLGVFAVSAAAGYRFGLDDRSRKYLAVDTDGDDLDDSVMLYKD
                     WSALTEASGGPQYTTTQFDQKDLAFSHRVDLDVLARLDLSKKLELLLDVGYTPVDATM
                     REYYTHVLNTQVLDDKSVNEELLLSDFGNVDAVATFRLKELKKGLAWRYALGYARTGY
                     TLKRDGAQASGLAFYSSENPGNFPELSLGLNPVNGSIVSSGEDPSAWVTHSALVRLGL
                     EWKPAKDVALTSSLGLTGSVSTLYYRAFNTDTRSVWQETVASDSIALGLSPKLCLTVP
                     AGEKALLSFSLAPDTGSGSYARGRETAAFDLTLNRQSDDGSVDLTGSSELSLSLGLSL
                     VIRR"
     gene            3170..3307
                     /locus_tag="PLQ29_05380"
     CDS             3170..3307
                     /locus_tag="PLQ29_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86113.1"
                     /translation="MVLVVVLGVQRLVVQAGEHGRVVLGRRGLVGQQRYDGGHAAGHA
                     S"
     gene            complement(3315..>4631)
                     /locus_tag="PLQ29_05385"
     CDS             complement(3315..>4631)
                     /locus_tag="PLQ29_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86114.1"
                     /translation="KNRVALAMIAAALIASAVSCSFLLGPEPTESMIDYYSKTVEYSR
                     YVDPAQNYYVPVERRMYDRYGRLSGIYRHTYEPVAGDTDYWVLKRTDLYEVSTTGVEG
                     LAEYYVYSYVPDDYTAFDDDDVAYAVHDYKLTHGDSYTPAGYNWHYYAVAYQDLPNQP
                     DRYTSIVDYARDSGGAFGVIARQESTYRLIYEDPVLDYDYYDYVTEKFFLSSTPGGTL
                     KLSEEFASWYNATAQWTHEIYHTFRGDDAANEVWYYTKYDWNEAGQVFAQQDFYYDPS
                     DITTIATYGVQTISPAWINPAGAMPSLTFANIGALAESLTKSYDELGNVSLKVRSALG
                     AEYERIVYRYEGDKETSMLRYVNGKHYAYDRTETRYYDIDRDGVTYGVAEELVYHTGE
                     TGEADEESASMHARWSRALSGIGRGSAISSIINNPIVRPLRSNHDR"
BASE COUNT          798 a         1428 c         1645 g          760 t
ORIGIN      
        1 gcgcttcagg cggtagaaca ggcgcagcag tatatcgccg aaggctacct cgccgagccg
       61 gccgcccagt ggcccgcgat aggcctcgag gtcggggaac tcgagcaggc cgaagttctc
      121 ccagacgggg gcctgacagg ccgcggccac gagcatgacg gccagcgcga ggccggcggt
      181 cgcgatagtg cgtattcgcc tcatgcggag gctccttcct tgcgccgatt cctgggggag
      241 ccgacgatgc cgaagccgtt gacgccgaat agcacgaagc cgaagctcgc tatcggcagc
      301 acgatggccc aggcgccgtg ctcggcgttc aggagcagcg cgttgtacaa ggaatgaacg
      361 gcgcagccgc caagcagggt gcccagcgcc agagccggcg agaatctgga tcgattgacc
      421 atcagctgga aacagaagcc ggagagggcc gtcgtcgagc cgtgcaggag cgaggcggtc
      481 aggctacgta gcagggtaaa gacgatggtc gacgagacgc ccgagtcggc cgccatggtc
      541 aggtacatgt agttctccag tatggagaag cctatgccta tggccatcgc gtagcgtacc
      601 agagagtact tgcccgtctt cttgctccgc aggagcaggt agacgagcgg caggaccttg
      661 gccgcctcct cgatggccgg gatgacgaag gccagctgca taggcagggg cacgacggcc
      721 tcgcttatga agccggtcag accgtagacc gccagggccg cgctcagccc gaacgcgaac
      781 gaggccatca ccccccgccc ttcgccggtc acgacgagga ggaggaaggc cagcggcacc
      841 acgaagctca ggtagacctg tatcatcatc acgggcgggc ctccgcgtcc gacagggata
      901 gggccgcgat cgcggcgacg gcgcagaccg acggggcgtg gcgccaagcg gccgggatcc
      961 aggcggcggc cgcctcgggg aggaggccgg cggcgtacgc ggccaggtaa gccagccagt
     1021 acgcgacgag cggcgccagc caggccgccg acagccgcct gaagcgtagc gccgcgatcg
     1081 cgacgacgag ggtctgcggc gcgaggagag cgagcgcgta gtagccccgg gcgccgtctg
     1141 gtctggcgta gagcgccaag gcgacgacga gcgccaccaa gacggcggct atcgtcgcgc
     1201 gccgtccgac gggcgcctct acgagcgctt cgtcgcccgg gtagaggccg gccctaccca
     1261 cccagatcag cgagacgacg acgccggtcg gcgaggccgc ctgtttgacc gcctcgggta
     1321 cgaactcgag ccgcccggac agcgcataga gcgagcccga ggcggccgcc gcgagcatcg
     1381 ccagcatcgc gagcgccatg gagccttcaa ggcgcctcgg gccggccgcg atcagcatcg
     1441 cggccagcaa gacgatcggc agcccgaagt cgagccagaa cagtatcagc tcggacggtc
     1501 cgccgagccc ggcgatcgcc tcgaatcttc caggcgcgac cagcaccgcg taggccaggg
     1561 ccaggaaggc cccgaggcta gcgtacagcc aggtcggcat cgacggcgcg gtgagcggcc
     1621 gcttcggagc ggccgcggag gctccgctca tcggcgtatc accaacgaga ggccgaggct
     1681 cagggacagt tcgctcgagc cggtcaggtc gacgctgccg tcatcggact gccgattgag
     1741 ggtcaggtcg aaggcggcgg tctcgcgtcc gcgggcatag gagcccgagc cggtatcggg
     1801 ggccagcgag aagctcagga gcgccttctc gccggccgga acggtcaggc acagcttggg
     1861 cgacagtccg agcgctatcg agtcagaggc gacggtctcc tgccagacgg agcgggtgtc
     1921 ggtattgaaa gcgcggtagt acagggtgga gacggagccg gtcagcccga gcgacgaggt
     1981 cagcgccacg tccttggcgg gcttccactc gaggccgagc ctgaccaggg cgctatgcgt
     2041 cacccaggcc gatgggtcct cgccgctcga tacgatggag ccgttgaccg ggttcaggcc
     2101 caggctcagc tcggggaagt tgcccgggtt ctcgctgctg tagaacgcga gtccggaggc
     2161 ctgggcgccg tcgcgcttga gcgtatagcc ggtccgagcg tagccgaggg cgtagcgcca
     2221 ggccaggcct ttcttgagct ccttgagcct gaaggttgcc accgcgtcga cgttgccgaa
     2281 gtcgctcagg agcagctcct cgttaacgct cttgtcatcg agcacctggg tattgagcac
     2341 gtgggtatag tactcgcgca tcgtggcgtc gaccggcgta tagccgacgt cgagtagcag
     2401 ctccagcttc ttggacaggt cgagcctggc gaggacgtcg aggtcgacgc ggtgcgagaa
     2461 cgccaggtcc ttctggtcga actgggtcgt cgtgtactga gggccgcctg aggcctcggt
     2521 cagggcggac cagtccttat acagcatcac cgagtcatcc aggtcgtcgc cgtcggtatc
     2581 gacggccagg tacttgcggc tgcggtcgtc gaggccgaag cggtagcccg ctgccgcgct
     2641 cacggcgaag acgcccagct tgccagacca gtcgccgcgg agcgtggcgg cgtgcgacat
     2701 gacgtccgcg tcgttcgaca cggcgtcggc gtacttgagc agcggctcct ggctcgtgtc
     2761 ggtcacccag cggtagctgt taggcgtata cgagaagtcg tagtcgagcg agtagcccga
     2821 cgacgatgac ttgcccttat tcgccaggag ccccttgagg ccgaactcga gcggccagtc
     2881 gtagagctgg gtcagcttgt actgggtcgg cgagtcgtag cggtcgagca gttcgtacgc
     2941 ggacgaaagg tcgcccgaga agtcgaagcc caggaggtag gtgccgccgg tccggggctt
     3001 ggcgagcgcc aacgacaggt ctccgccgag gcctatggcc cggttcaggt cggaaccgcc
     3061 ggcgatgcct accgagtcgt cgagcctcgt ggccctggta tccgagtaat cgaagtacgc
     3121 gttgccgtcg atgacggtag gcgcccggga gcgagctgat ggccgtagga tggtcctcgt
     3181 agtcgtactc ggggtacagc gcctcgtcgt acaggccggc gaacacggcc gggtagtcct
     3241 gggccgtcgc ggcctggtag gccagcagcg ctatgacggc ggccatgccg cgggccacgc
     3301 gtcgtgaagc tatgttagcg gtcatggttg ctcctcaggg gacggacgat gggattgttt
     3361 atgatcgacg atatggccga gccgcggccg atcccggaca gcgcccggct ccagcgcgcg
     3421 tgcatcgagg ccgactcctc gtcggcctcg ccggtctcgc cggtatggta gaccagctcc
     3481 tcggccaccc cataggtgac gccgtcgcgg tctatgtcgt agtaacgcgt ctcggtgcgg
     3541 tcgtaggcgt agtgcttgcc attgacgtag cgcaacatcg aggtttcctt gtcgccttcg
     3601 tagcggtaga cgatgcgctc gtactccgcg ccgagcgcgc tacgtacctt gagcgatacg
     3661 ttaccgagct cgtcgtagct cttggtcagc gactcggcga gcgcgccgat gttggcgaag
     3721 gtcaaggacg gcatggcgcc tgccgggttg atccaggcgg ggctgatggt ctggacgccg
     3781 taggtggcta tcgtcgttat gtcgctcggg tcgtagtaga agtcctgctg ggcgaagacc
     3841 tggccggctt cgttccaatc gtacttggtg tagtaccaga cctcgttggc ggcgtcgtcg
     3901 ccccggaagg tatggtagat ctcgtgggtc cattgggccg tggcgttata ccagctggcg
     3961 aattcctcgc tcagcttgag ggttcccccg ggcgtgctcg aaaggaagaa cttctcggtc
     4021 acgtagtcgt agtaatcgta atcgaggacc ggatcctcgt agatgagccg gtaggtgctc
     4081 tcctgcctgg ctatgacgcc gaaggcgccg ccggagtccc gggcgtagtc gacgatgctc
     4141 gtgtagcggt ccggctggtt cggcaggtcc tggtacgcca ccgcgtagta atgccagttg
     4201 tagccggccg gcgtgtagct gtcgccgtgg gtcagcttgt agtcatgtac ggcataggcc
     4261 acgtcgtcgt cgtcgaacgc cgtgtagtcg tccggcacgt agctgtacac gtagtactcg
     4321 gccaggccct cgacgccggt ggtcgagacc tcgtagaggt cggtgcgctt gagcacccag
     4381 tagtcggtgt cgccggcgac cggctcgtag gtatggcggt agatgccgga gagcctccca
     4441 tagcggtcgt acatgcggcg ctcgaccggc acgtaatagt tctgcgccgg gtcgacgtaa
     4501 cggctgtact cgacggtctt ggagtagtag tcgatcatcg actcggtagg ctcaggcccc
     4561 aagaggaagc tacaggagac ggccgaggcg ataagcgccg ccgctatcat ggctaacgct
     4621 acccggttct t
//
LOCUS       DAOPHV010000155         4603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_25205_length_4603_cov_4.652595, whole genome shotgun sequence.
ACCESSION   DAOPHV010000155 DAOPHV010000000
VERSION     DAOPHV010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25205_length_4603_cov_4.652595"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLQ29_05390"
     CDS             complement(<1..350)
                     /locus_tag="PLQ29_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86115.1"
                     /translation="MRLRFVSGVVVSACAVACAVYLSGGRVVPGYVALDLALITLLPL
                     GAAMAAFGPRAVGRAFTVTTDSIAAGASERRDAALRDAALRDADVMLRWLARATWLAM
                     AAGFLLIQIWMMYDL"
     gene            complement(385..801)
                     /locus_tag="PLQ29_05395"
     CDS             complement(385..801)
                     /locus_tag="PLQ29_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86116.1"
                     /translation="MTGKDVDRLLGAYYARRLEKGDGLYGKDDGLARRVGRGERLKPV
                     DCIRADAARADAARADAARADAAHADAARADGRLSAVLAAAALVMMLGFGVLGSPSGD
                     ESGARNGLVASMSLDLAGSRFEEAVIAIGDSWPRKN"
     gene            complement(798..1376)
                     /locus_tag="PLQ29_05400"
     CDS             complement(798..1376)
                     /locus_tag="PLQ29_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPG86117.1"
                     /translation="MNMRNDHGCLDGWPELRPRLELYLASFSALGAEDREDALQATMI
                     AYGAGGASAGGDRRAWAYRVARNAAIDAIRRRNRERRLIERVALGERVALGVGEALAA
                     TEEAGGRSVEPESPYRGPEAQAIDAEDEAFVARFIAGLGDAEREMLHLAYAEGLTYAE
                     IAATVGAPLGTVKWRLASVKRRLKAAYEREYL"
     gene            1488..2951
                     /locus_tag="PLQ29_05405"
     CDS             1488..2951
                     /locus_tag="PLQ29_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPG86118.1"
                     /translation="MHVVGIVALVLGSALLALLATMLARAAACHRAPAPGRLDASRLD
                     AYAGAEGRLAALIRVPTVSRFDPAEEDDGAFTRLKAEALRLYPLAASRLLRSEPGDRA
                     MLFEWPGRDASLEPVILTAHYDVVPPGDEGAWDRPPFSGEIADGYVHGRGAQDVKIMM
                     AAALEAAERLLRSDFVPRRTVFLAFGGDEETGGVRGAASIAAVLRERGVRASFLLDEG
                     GFVAEGMLGFADRPLALVGVAEKGYVDVAIVASGSAGHASMPPRHTAAGLVAKAVSLS
                     EARPFPARITATLARFLGDLAPYAPFGYRLLFRNLIVTAPLVKAAFSAAPTTNALVRT
                     TCAATMLSGSDKENVLPDRASAVLNVRVLPGDDVAGTMARLDAIAARAGARADFAHHG
                     YAVEPLPESPVDHDGYRLLKAAIGEVFPEAGAVPFMFTAGTDTKHYVGLCEAIYRFEP
                     ILQSADDLKGVHGPNERVSVDNARRCCLFYETLLRSL"
     gene            2948..3520
                     /locus_tag="PLQ29_05410"
     CDS             2948..3520
                     /locus_tag="PLQ29_05410"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPG86119.1"
                     /translation="MTPGDDGGTGYELIRPGLEQLRTLYDWKAAESRRERFTCRPVKP
                     LGPFDEYAAKLSSWLREPMNLLFALVASGSPEPLGEVKGFDWNERNHSLEFGYYLPPG
                     NRGKGYGRILARLFVERVFGDGEAGVNKLYATTSAENAPSIRTLEGLGFRLDGRNREH
                     YWMDGERYDQCVYSLLASEWLASGDERRGP"
     gene            complement(3517..4200)
                     /locus_tag="PLQ29_05415"
     CDS             complement(3517..4200)
                     /locus_tag="PLQ29_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86120.1"
                     /translation="MMGGPVDRPAARALALCMATLCAAICVGSCGRGDDGPHVAARAA
                     AADPLAAPGAPGRPDAPGRPDAPGRTDAPGRPGVGDVTLGPGAALLIEGSAAVSGLPG
                     FGRNAFKGLRGVYAYRPDEGEARYALRVWYTSEPLLLGDGWAAVACPRLPSPWRALAA
                     AAHESAPEGSSFWALSSGGTTLLMEFPEDMPEPCRFAAALVDRFAFFSRYAQGPGDLS
                     FPATLEVGR"
     gene            complement(4197..>4603)
                     /locus_tag="PLQ29_05420"
     CDS             complement(4197..>4603)
                     /locus_tag="PLQ29_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPG86121.1"
                     /translation="LYPIALEGALKLKEISYIHAEGYASGEIKHGPIALVSPETPSVF
                     LVSNDHLREKTISNIREIKARGGPVIALAQEGDDEVAGLADEFIPVPAVGSRFQPFVM
                     VPPLQLLSYFCALELGRNVDQPRNLAKSVTVE"
BASE COUNT          694 a         1675 c         1569 g          665 t
ORIGIN      
        1 agatcgtaca tcatccatat ctggatgagc aggaagccgg cggccatcgc gagccaggtc
       61 gcccgggcca gccagcgcag catgacgtcc gcgtcgcgca gggccgcgtc gcgcagggcc
      121 gcgtcgcgtc tctcgctggc gcccgcagct atcgagtcgg tagtgacggt gaaggccctt
      181 cctacggctc gaggcccgaa ggccgccatg gcggcgccga gaggcaggag cgtgatcaag
      241 gccaggtcga gggccacgta gccgggaacc acgcgtccgc cggacaggta cacggcgcag
      301 gcgacggcgc aggccgatac gacgacgccg gaaacgaatc tcagtctcat gttttcctcc
      361 atcacacgct ttaagaaagc gctatcagtt cttgcgcggc cagctatcgc ctatggcgat
      421 gacggcctcc tcgaaacgcg aaccggccag gtcgaggctc atcgacgcga ccaggccgtt
      481 tctcgcgccg ctctcgtcac cgctcgggga gccgagaacg ccgaagccta gcatcatgac
      541 cagcgccgcc gcggcgagga cggccgacag ccggccatcg gcgcgcgcgg cgtcggcgtg
      601 cgcagcgtcg gcgcgcgcag cgtcggcgcg cgcagcgtcg gcgcgcgcag cgtcggcgcg
      661 aatacagtct accggcttca ggcgctctcc gcggccgacg cgccgggcca gtccgtcgtc
      721 ctttccatag agcccgtccc ccttctcgag cctccgcgcg tagtacgcgc cgagcaggcg
      781 gtcgacgtcc tttccagtca taggtactcc cgttcatagg cggccttgag ccgacgcttg
      841 accgacgcca gccgccactt gaccgtgccg agcggagcgc cgacggtggc cgctatctcc
      901 gcgtacgtca aaccctcggc ataggccagg tgcagcatct cgcgctcggc gtccccgagc
      961 cccgctatga agcgtgcgac gaaggcctcg tcctcggcgt cgatcgcctg ggcctcgggt
     1021 ccgcggtacg gcgactcggg ctccacgctc cggccgccag cctcttcggt agcggcaagc
     1081 gcttcaccaa cgccgagcgc tacgcgctcg ccgagcgcta cacgctcgat gagcctccgc
     1141 tcccggttac gccgcctgat agcgtcgatc gcggcgttcc tggccacgcg gtaggcccag
     1201 gcccgacggt cgccgccggc tgacgcgccc ccggctccgt aagcgatcat cgtcgcctgc
     1261 agggcgtcct cccgatcctc ggctccgagg gcggagaagg aggcgaggta gagttccagt
     1321 cgcggccgca gctcgggcca gccgtcaagg caaccgtggt cattgcgcat gttcatcgca
     1381 tactaccacg ctcgagcctc gaatccggtt ggttcataat cgcgcttcct atagcccgga
     1441 cggaaggcta tactggtggc agtcgtcaaa cgaagcggga ggcaagcatg cacgtcgtcg
     1501 gaatcgtggc cctggtcctg ggatcggccc tgctcgccct gctcgcgacg atgctcgccc
     1561 gcgccgccgc ctgccatcgc gcgccggcgc cgggccggct cgacgcttcg cggctcgacg
     1621 cgtacgccgg agccgagggc cgcctggccg cgctgatacg cgtgccgacc gtatcgcgct
     1681 tcgacccagc cgaggaggac gacggcgcct ttacccggct caaggccgag gcgttgcgcc
     1741 tctacccgct cgccgcctcg cgcctcctac ggagcgagcc gggcgaccgc gcgatgctgt
     1801 tcgagtggcc gggccgcgac gcctcgctgg agccggttat cctgacggcc cactacgacg
     1861 tggtgccgcc cggcgacgag ggggcctggg accggccgcc gttctcgggc gagatcgccg
     1921 acggctacgt gcacggccgc ggcgcccagg acgtcaagat catgatggcc gccgcgctcg
     1981 aggcggccga gcgcctcctc cgctcggact tcgtaccgag gcgtaccgtg ttcctggcct
     2041 tcgggggcga cgaggagacc ggcggcgtgc gcggcgccgc gtccatcgcg gcggtcctgc
     2101 gcgagcgcgg cgtccgcgcg tcgttcctgc tcgacgaggg tggcttcgtc gccgagggca
     2161 tgctcggctt cgccgaccgc ccgctcgccc tcgtaggcgt cgccgagaag ggctatgtcg
     2221 acgtcgcgat cgtcgcctcg ggctcggccg gacacgcctc gatgccgccg cgtcacacgg
     2281 ccgccggcct ggtcgccaag gccgtctccc tgtccgaggc cagacccttc ccggccagga
     2341 tcaccgcgac cctggcgcgc ttcctcgggg acctggcgcc ctacgccccc ttcggctatc
     2401 ggctgctgtt caggaacctg atcgtcaccg cgcccctcgt caaagccgcc ttctccgccg
     2461 cgccgacgac caacgccctc gtgcgcacga cctgcgccgc gacgatgctg tccggctccg
     2521 acaaggagaa cgtgctgccc gaccgcgcca gcgcggtgct gaacgtgcgg gtgttgcccg
     2581 gcgacgacgt ggccgggacg atggccaggc tcgacgcgat cgccgccagg gccggcgcca
     2641 gggccgactt cgcccatcac ggctacgccg tcgagcccct gcccgagtcg ccggtagacc
     2701 acgacggcta tcgcttactg aaggcggcca tcggcgaggt cttcccggag gccggcgcgg
     2761 tgccgttcat gttcaccgcg ggcaccgaca ccaagcacta cgtcgggcta tgcgaggcga
     2821 tctaccgctt cgaacccatc ctccagtcgg ccgacgacct caagggagtg cacggcccga
     2881 acgagcgcgt ctcggtcgac aacgcgcggc gttgttgcct gttctacgag acgctcctga
     2941 ggtctctatg acgcctggcg acgacggcgg caccgggtat gagctcatac ggccgggcct
     3001 cgaacagcta cgtacgctgt acgactggaa ggccgccgag agccggcgcg agcgcttcac
     3061 ctgcaggccg gtcaagcctc tcggcccctt cgacgagtac gccgccaagc tgagttcgtg
     3121 gctacgggag cctatgaacc tgctcttcgc gctcgtcgcg tcgggatcgc cggaaccgct
     3181 cggcgaggtg aagggcttcg actggaacga gcgcaaccat agcctcgagt tcggctacta
     3241 cctgcctcca ggcaaccggg gcaagggtta cggcaggatc ctggcgcgtc tcttcgtcga
     3301 gcgcgtcttc ggcgacgggg aggccggcgt caacaagctc tacgcaacca cctcggccga
     3361 gaacgcgccg tccatccgta cgctcgaggg tctcggcttc aggctcgacg gaaggaatcg
     3421 cgaacactat tggatggacg gcgaacgcta cgaccaatgc gtctattcgc tcctagcaag
     3481 cgagtggctg gcctccggcg acgagcggcg aggcccctaa cgcccgacct cgagcgtagc
     3541 cggaaacgat aggtcgcccg gcccctgggc gtagcggctg aagaaggcga aacggtcgac
     3601 gagggccgcc gcgaagcggc agggctcggg catatcctcg gggaactcca tgagcagcgt
     3661 cgtccctccg gacgatagcg cccagaacga tgagccttca ggcgcggatt catgcgcggc
     3721 cgccgcgagc gcccgccagg gcgagggcag ccgagggcat gcgaccgccg cccagccgtc
     3781 gcccaagagg agcggctcgg aggtatacca gacgcggagc gcgtaccgcg cctcgccctc
     3841 gtccggccgg taggcgtaga cgccacgcag gcccttgaac gcgttccggc cgaagccggg
     3901 cagcccgctc accgcggcgg aaccctcgat cagcagggcc gcgcccggcc cgagcgtcac
     3961 gtcgccgacg cctggccgcc cgggagcatc tgtccgcccg ggagcatctg gccgcccggg
     4021 agcatctggc cgcccgggag cgccgggagc cgccagcggg tcggcggcgg ccgcccgggc
     4081 cgcgacgtgg ggcccgtcgt cgccgcgacc gcacgagccg acgcagatcg cggcgcagag
     4141 cgtcgccata cagagcgcca gggcccgggc cgccgggcga tcgacgggtc cgcccatcat
     4201 tccaccgtca cgctcttggc caggttgcgc ggctggtcga cgttgcgccc gagctcgagc
     4261 gcgcagaagt acgagagcag ctgcaggggc ggcaccatca cgaagggctg gaagcgagag
     4321 ccgaccgccg gcaccgggat gaactcgtcg gccaggccgg ccacctcgtc gtcgccttcc
     4381 tgggccagcg ctatcaccgg gcccccgcgc gccttgatct cgcggatgtt cgagatggtc
     4441 ttctcgcgca ggtggtcgtt ggacacgagg aacacgctcg gcgtctccgg gctgacgagg
     4501 gctatcggcc cgtgtttgat ctcgcccgag gcgtagccct ccgcgtggat gtacgaaatc
     4561 tccttgagct tgagcgcgcc ctcgagggct atcgggtaca gga
//
LOCUS       DAOPHV010000156         4602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_25216_length_4602_cov_5.428194, whole genome shotgun sequence.
ACCESSION   DAOPHV010000156 DAOPHV010000000
VERSION     DAOPHV010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25216_length_4602_cov_5.428194"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1717
                     /locus_tag="PLQ29_05425"
     CDS             <1..1717
                     /locus_tag="PLQ29_05425"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HPG86122.1"
                     /translation="PKPFSVVFLTAAAGPGTRAAPFGAARVASALKAWSAPQPAASAG
                     SNDAPSGPDRPIHASIVEAEPDESPEALAARTLALSPDLVGLSLYSWNSVRLEAAGAA
                     LKAARPGLVIVAGGPDASADPEALAARGPADVVVAGEGERVIVDLVAAMLSGKPLPGP
                     VVRAAPVDPAGLSSPWLDGTLDPGRWGGGALELTRGCPYRCAFCFESKGEARLRRFPL
                     ETAGRELERFEAAMVEEVFVLDPTFNADPKRMAAAVRSFSERGPNLRYFLEIRAELLS
                     AEQARLLGSIDCSVQIGLQSADPAVLALVDRTLDPALFARKTRLLDEEGVVYGLDLIY
                     GLPGDTLAGFRRSLDYALGLGPNHLDVFRLAVLPGTALHDRASGLGLDWDRSTPYLVR
                     STPSFGDAELDRAERLAVATDVLYTRGRAVMWFRSVAALAKAKPSALLAAFADFMDQE
                     RSGPLAGIIAEARADAARHDDIELLQTAFFGDWFKRRPTRKPDHALAAVDLIRASGAW
                     TRAFAEGEPTTLELGWPPEDLLDYASFDVAEFADETSRSPRRWTCEPGRDGPRFAPAK
                     VKPGR"
     gene            complement(1714..2697)
                     /locus_tag="PLQ29_05430"
     CDS             complement(1714..2697)
                     /locus_tag="PLQ29_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPG86123.1"
                     /translation="MNPVVAAIADTPLFAGMSGLELNAVSAFLEPRRYAAGAVIFRRG
                     EPGTEMFIVRAGRVASLALDRDGNERQLYEFGPGRFFGEMAIIENEPRSATCVALETT
                     ELLVLEGIDFYRLVWEHPMIGSRMLASMAKVMAGWLDEASGFLTDLVRWGETARKRAV
                     TDELSGLFNRRFIEEASRSRFARNSSEPPLCSALMLDLDRFHALNERFGTMAGDSIIS
                     TAGAAYSRLIRDGDVAARLAGDEFAFFMPDAGPEEALAAAERIRSETESLYLEFRSGP
                     GATPQRVSLSASIGVASSPFDATDSAGLFAAADKALFSAKEAGRNRVVRFR"
     gene            complement(2694..3719)
                     /locus_tag="PLQ29_05435"
     CDS             complement(2694..3719)
                     /locus_tag="PLQ29_05435"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPG86124.1"
                     /translation="MDGKEYGLADIVPLLKRSDFFSRLLPDDMYWLASKSELVARPSG
                     AVLFRPGDRAVRFFIVKSGSVAVSRLDAGGRAEEMARFVAGDVVGDFDFSRSAAYDAA
                     AVCAEDSEILSFPGRGATMDDLARERPDVSARILLRTVAMISSRVRSTQALISDNAPW
                     VRELRRQMYTDSATGLWSRSFLDDELARSIEPPAAVLLCKPDRFKELCDGWTHAAGDA
                     AMERIAALLKDEARSLRRAWAVRMRSNETAVIAAGYGLEQAAALAERIRAAYAAMDLS
                     PITGDSGFHLSASVAIAVWPDDGTDFKELVEQAYGLMTRAWKDGGDRVYRLGSFEPSP
                     GGPEGRR"
     gene            4048..>4602
                     /locus_tag="PLQ29_05440"
     CDS             4048..>4602
                     /locus_tag="PLQ29_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86125.1"
                     /translation="MKRTILVVALLAAASASSFAEDSVLDLFFPDHTSSIAYFDAGVV
                     YAPIGTVASGGIGLNFNAGFNIGRLFHEKLVLSPFAGVTPCFLNAIKPGFIRDLADNY
                     ETPERLAYLQEHQDIEEERAYLDATSLLDYLMAGEALEKFSITMRYGVLVQLPWRFFP
                     PIALYGLYSGTAIVGSETRTGYDTV"
BASE COUNT          735 a         1614 c         1528 g          725 t
ORIGIN      
        1 cccgaaaccg ttctcggtcg tcttcctgac ggcggccgcg gggccgggca cgcgggccgc
       61 gccgttcggt gccgcccgcg tcgcgtcggc gctcaaggcc tggtccgcgc cacagcccgc
      121 ggcgagcgcc ggatcgaacg acgccccatc cggccccgac cgtccgatac acgcgtccat
      181 cgtcgaggcc gagcccgacg agtcgcccga ggccctcgcg gcccggacgc tcgccctctc
      241 gcccgacctc gtcgggctgt cgctgtactc gtggaactcg gtccggctcg aggccgccgg
      301 ggcggctctc aaggccgctc gccccggcct cgtcatcgtc gccggagggc cggacgccag
      361 cgccgatccc gaggcgctcg cggcgcgcgg cccggccgac gtcgtggtgg ccggcgaagg
      421 cgaacgggtc atcgtcgacc tcgtcgccgc catgctctcc ggaaagccac tgccgggacc
      481 cgtcgtgcgg gccgcacccg tggacccggc cggcctatcg tcgccctggc tcgacggcac
      541 cctcgacccc gggcgctggg gcggcggcgc gctcgagctg acgcgcggct gtccctaccg
      601 ctgcgccttc tgcttcgagt cgaagggcga ggcgaggctc aggcgcttcc cgttggagac
      661 ggcgggccga gagctcgagc gcttcgaggc ggcgatggtc gaggaggtct tcgtgctcga
      721 cccgaccttc aacgccgacc cgaagcgcat ggcggccgcc gtcaggtcgt tctccgagcg
      781 cggtccgaac ctacggtact tcctggaaat ccgcgccgag ctactaagcg cggagcaggc
      841 caggctcctc ggctcgatcg actgttcggt gcagatagga ctccagagcg ccgacccggc
      901 ggtgctggcc ctcgtcgacc ggacgctcga cccggccctg ttcgcgcgga agaccaggct
      961 actcgacgag gagggcgtcg tctacggcct cgacctgatc tacggcctgc ccggcgacac
     1021 gctcgccggc ttcaggcgca gcctcgacta cgccctcggc ctcggcccga accacctgga
     1081 cgtgttcagg ctggcggtac tcccgggcac ggccctccac gaccgcgcct cgggcctcgg
     1141 gctcgactgg gatcgctcca ctccctacct cgtgcgctcg acgccgagct tcggcgacgc
     1201 cgagctcgac cgggccgagc ggctcgccgt cgcgaccgac gtcctctaca cgcgcggccg
     1261 agccgtgatg tggttccgct cggtcgcggc gctggccaag gccaagccgt ccgccctgct
     1321 cgccgccttc gccgacttca tggaccagga acgatccggg ccgctcgccg gcatcatcgc
     1381 ggaggcgagg gccgacgcgg cgcgccacga cgatatagag ctgctgcaga ccgcgttctt
     1441 cggcgactgg ttcaaacgca ggccgacgag gaagcccgac cacgcgctcg ccgccgtcga
     1501 cctgatccgc gcctccggcg cctggacgag ggccttcgcc gagggcgaac cgacgacgct
     1561 ggagctcggc tggccgccgg aggatctact cgactatgcc agcttcgacg tcgcagaatt
     1621 cgccgacgag acgagtcgtt cgccgcggcg ctggacctgc gaacccggcc gagacgggcc
     1681 gcgcttcgcg ccggccaagg tcaagcccgg ccgctagcgg aagcgcacga cgcggttgcg
     1741 gcccgcttcc ttggcgctga acagggcctt gtcggcggcg gcgaacaggc cggccgaatc
     1801 ggtcgcgtcg aaggggctcg aggccacgcc gatgctggcg ctcaggctga cgcgctgggg
     1861 ggtagccccc gggccggagc ggaactccag gtacagcgac tcggtctccg agcggatgcg
     1921 ctcggcggcg gccaaggcct cctcggggcc ggcatccggc atgaagaacg cgaactcgtc
     1981 gcccgccagt ctcgcggcca cgtcaccgtc gcgtatcagc cggctgtacg cggccccggc
     2041 ggtcgagatg atcgagtcgc cggccatcgt cccgaagcgc tcgttgagcg cgtgaaagcg
     2101 gtccaggtcg agcatcagcg cggagcatag cggcggctcc gaagagttgc gcgcgaagcg
     2161 cgagcgcgag gcctcctcga tgaagcggcg gttgaacagg ccggatagct cgtcggtcac
     2221 cgcgcgcttc ctggccgtct cgccccagcg caccagatcg gtcaggaagc ccgaggcctc
     2281 gtccagccag ccggccatca ccttggccat cgaggcgagc atcctcgagc ctatcatcgg
     2341 atgctcccag acgaggcggt agaaatcgat gccctcgagc accaggagct cggtcgtctc
     2401 gagcgccacg caggtggccg agcgcggctc gttctcgatg atggccatct cgccgaagaa
     2461 gcggcccggg ccgaattcgt agagctggcg ctcgttgccg tcgcggtcga gggccagcga
     2521 cgcgacgcgc ccggccctca cgatgaacat ctccgtgccc ggctcgccgc gcctgaagat
     2581 cacggcgcct gccgcatagc gcctcggctc caggaaggca gagacggcgt tcagctcgag
     2641 gccgctcatg ccggcgaaca gcggggtgtc ggctatcgcc gcgacgacgg ggttcatcga
     2701 cgcccctccg gcccgccggg actcggctcg aagctaccga gccggtagac cctgtcgccg
     2761 ccatccttcc aggcccgcgt catcagcccg taggcctgct cgacgagctc cttgaagtcc
     2821 gttccgtcgt cgggccagac ggctatcgcg acgctggcgc tcaggtgaaa accggagtcc
     2881 ccggtaatgg ggctcaggtc catcgcggcg taggcggcgc ggatccgctc ggccagggcc
     2941 gcggcctgct cgagcccgta gccggcggct atcacggcgg tctcgttgct gcgcatcctg
     3001 acggcccaag cccgcctgag cgatcgggcc tcgtccttga gcagggccgc gatgcgttcc
     3061 atcgccgcgt ctccggccgc gtgagtccag ccgtcgcaca gctccttgaa gcggtccggc
     3121 ttgcagagca gcacggcggc cggcggctcg atcgagcggg ccagctcgtc gtcgaggaag
     3181 gagcgcgacc aaaggccggt cgccgagtcg gtgtacatct ggcgcctgag ctcgcgcacc
     3241 cagggcgcgt tgtccgatat gagcgcctgg gtcgatcgca cccgcgacga tatcatcgct
     3301 acggtgcgga ggaggatgcg cgccgacacg tcgggacgct cgcgagccag gtcgtccatc
     3361 gtcgccccgc ggcccgggaa cgacaggatc tccgagtcct cggcgcagac ggcggcggcg
     3421 tcgtaggcgg ccgagcgcga gaagtcgaag tcgccgacca cgtcgccggc cacgaagcgg
     3481 gccatctcct cggcacggcc gccggcgtcc agcctggaca ccgcgaccga gcccgacttg
     3541 acgatgaaga aacgcaccgc ccggtcgccg ggcctgaaca gcacggcgcc ggacggacgg
     3601 gcgaccagct ccgacttgga ggccagccag tacatatcgt ccgggaggag gcgcgagaag
     3661 aaatccgaac gcttgagcag cggcacgatg tcggccagcc cgtactcttt cccatccatg
     3721 ctccgtgaag tataaacggc aatccgccgt aatggaatag aatcggagcg cccgggcgct
     3781 cttcatcgtt tcgagacaat gataaagccc cggcagggtt aatccttccg gtcatggagt
     3841 tgcctgtatg agtaagggtt ttctaggaaa cgccttttcg acttcgcttg acaaataaat
     3901 atagctacct aagctacttg acgagaagct tgatttaagg aagcgaactt cagtcccgcc
     3961 cgtttccata gccgctcgac tgaatttcgt cgcttgggtc gtgccggagc catatacgaa
     4021 acgttttgcc aaagccggag cataaaaatg aaacggacaa tacttgttgt agcgttactc
     4081 gcggcggcgt cagcctcttc attcgccgaa gattcggtac tggatttatt tttccccgac
     4141 catacttcga gcatcgccta cttcgacgct ggcgtcgtgt acgcgccgat cggtaccgtg
     4201 gcgtcgggag ggatcgggct gaacttcaac gcggggttca atatcggccg cctgttccac
     4261 gagaagctgg tcctatctcc tttcgcgggc gtcaccccct gttttctgaa tgccatcaag
     4321 cccggtttca ttcgcgacct ggcggacaac tacgagactc ccgagaggct agcctatctc
     4381 caagagcatc aggacatcga ggaggagcgc gcatacctgg atgccacctc gctgctcgac
     4441 tatctcatgg ccggcgaggc tctggagaaa ttttccatta ccatgcgcta cggcgtcctc
     4501 gtgcagctgc cctggcggtt ttttcctccg attgccctct acggcctgta tagcggaacg
     4561 gctatagtgg ggagcgagac gagaacggga tacgacacgg ta
//
LOCUS       DAOPHV010000157         4596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_25257_length_4596_cov_3.562872, whole genome shotgun sequence.
ACCESSION   DAOPHV010000157 DAOPHV010000000
VERSION     DAOPHV010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25257_length_4596_cov_3.562872"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PLQ29_05445"
     CDS             <1..834
                     /locus_tag="PLQ29_05445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPG86126.1"
                     /translation="AVVLAAAIVSSFAFDWPADSGRYRFSFGSPRDGFKRGLEFGSSG
                     GIVRAADDGELTFAASGRSLPGGYPLIGGSMVVVSHASQISTIYAGLEPGSVSSYLRN
                     VRKGDIIGRIEPVGGLGRGAAFYAYDAKERRYINPLILMPSLSDDKPPVIRSAILVSD
                     GKETALAASKVVKQGSYLLLVDAFDPSPTGEQAAPYEVRVMLDGSERDRVLYDAAWAE
                     GGRSTLFSGARLGEHEYLASDGRMRFGPYVLARGRVVISVVVGDFAGNKREQTYSIIV
                     Q"
     gene            974..1714
                     /locus_tag="PLQ29_05450"
     CDS             974..1714
                     /locus_tag="PLQ29_05450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPG86127.1"
                     /translation="MFRQDPQPELEAVLRRYGDEYLEYETERHLEYRAISLLSLEEAG
                     LRPEDAGRADGAPRSILEVGCATGALLSSFHEAGWRAQGVEVGPSMAGYARERFGLDV
                     ETATIESATYEIGSFDAVVATHLIEHLNDPRSFLARARAALRPEGRLYLITPNADGLQ
                     ARLMGSRWRSAIRDHLFLFSARTLGAMLADEGYYVEYVGTWGGWPAGMRPVSLKRPMD
                     RLAKRYGLGDVMVVRASQATVPTPLEVR"
     gene            1711..2424
                     /locus_tag="PLQ29_05455"
     CDS             1711..2424
                     /locus_tag="PLQ29_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPG86128.1"
                     /translation="MTDDYRITVAQAVENVLERMGAACARAGRSADEVRLLAVTKFHP
                     YEAVEAAYAAGVRLFGENRVQEAEAKFSAGTAPKGAFVHLLGHLQSNKAKRAAALFDC
                     VQSIDSIEVAAELAKRAAALGKTLDVLLELHTGEESKTGFASEDALLRALEAIMPMPS
                     LKPRGLMTMAPFSDDAAVVRASFRTCRRAFEVAMKRFAPVDFSVLSMGMSNDFELAVE
                     EGSTMVRVGTAIFGSRRLR"
     gene            2421..2816
                     /locus_tag="PLQ29_05460"
     CDS             2421..2816
                     /locus_tag="PLQ29_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86129.1"
                     /translation="MRLARSVAALSLALMAATAAFPQSASTPAESSLPEPYRAGEFPP
                     WLDAARRFEIVGLGVFPILLFYTRFASDAARYVRNGFDATYAPWPFKNEQSYEPSDDE
                     QWKNVLTAAGLSVAFAALDALVLWRVQLE"
     gene            2877..3947
                     /locus_tag="PLQ29_05465"
     CDS             2877..3947
                     /locus_tag="PLQ29_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPG86130.1"
                     /translation="MHRQAEARDATVLAVDVGGTNTSCAVVDVRDGYFTTTFERRYST
                     KAEPSLYEPLGRFIDEASSAGVALPELLCVSGAGPVVDRRIALTNAPWGIDGDGLSER
                     LGLPTYVINDLSAIAWGVLLLDPTDPNELAQLSAPDGAAPDPYGPVVVIGAGTGLGFG
                     YALRDDGSVRVYPSEGGHVCLPVYDDESRAFSRWLEARYGFAAGAEAGVSGQGIAAIF
                     EFLSASEPSLSEAARAALSAPAEDRPALVSTAADSGDALCLRVMDMFVRLYARVAADA
                     ASVFMPSGGIYLAGGIAAKNLARFTEGDRFMRSFGQGYREHVRAIAARTPVFVVKDYA
                     ISMYGAANAAVHLADGSRWRPC"
     gene            3932..>4596
                     /locus_tag="PLQ29_05470"
     CDS             3932..>4596
                     /locus_tag="PLQ29_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86131.1"
                     /translation="MAAVLSDALRSDMVAKGVDVETTLSILADLNAGLYDGQKAVRAT
                     GVPPVDGASIVPSAKDMAYRIGADEALSRLSDLGLELPDGARADGDSLLFDRAALEDL
                     GERLYPVAAWGVLNGGSATSYADRKKNAALGPGVFEAIRSGFELLAPLCDGRPKGVTP
                     AYINPDGSPGESFLLLKMRAALLRAERYVERFGMPARPALPFFQMTSDGTDAQLADAY
                     AVYA"
BASE COUNT          718 a         1554 c         1536 g          788 t
ORIGIN      
        1 gccgtcgtcc tggccgccgc catcgtgtcc tccttcgcgt tcgattggcc agccgattcg
       61 ggccgttacc gcttcagttt cggctcgcct cgggacgggt tcaaacgagg cctggagttc
      121 ggttcgtccg gcgggatcgt cagggccgca gacgacgggg agctgacctt cgccgcatcc
      181 ggtcggagcc tgcccggcgg ctatcccctg atcggtggct ctatggtggt cgtctcgcac
      241 gcctcccaaa tatcgaccat atacgccggc ctggagcccg gttccgtctc ttcgtacctt
      301 aggaacgtcc ggaagggcga tatcatcggc cgtatcgagc cggttggcgg ccttgggcgt
      361 ggggccgcgt tctacgcgta cgacgccaag gagcgccgct atatcaaccc gctcattctg
      421 atgcccagcc tctccgacga caagccgccg gtaatcaggt ctgccatcct ggtcagcgac
      481 ggcaaggaga cggctctcgc cgcgtccaag gtcgtcaagc agggatcgta cctgctgctc
      541 gtagacgcgt ttgacccgtc gccgaccggc gagcaggcgg cgccgtacga ggttcgggtc
      601 atgctggacg gttcggagcg ggatcgagtg ctgtacgacg ccgcctgggc cgagggcgga
      661 cgttcgactc tgttcagcgg ggccaggcta ggcgagcacg agtacctggc gagcgacgga
      721 aggatgcgct tcgggccgta cgtcctcgcc aggggccgtg tcgtcatcag cgtggtcgtc
      781 ggcgatttcg cgggcaacaa gagggagcag acatattcga tcatcgtcca gtgatggagc
      841 catacggacc ttctcgacga gcgcccgggc ggagccggcg cggccatcgc cgtgtccgct
      901 ctgcggctcg tccgctatcg gacgcggttg ggatgcagac ggcgctcggt tcgtacgatg
      961 cccgtcctgc ggtttgttca ggcaggaccc gcagccggag ctcgaggcgg tgctacgccg
     1021 ctacggcgac gagtacctcg agtacgagac cgagcggcac ctggagtacc gcgccatctc
     1081 gcttttgagc ctcgaagagg ctggccttcg gccagaggac gccgggcgcg ccgacggggc
     1141 gccgcgcagc atcctcgagg tcgggtgcgc gacgggagcc ctgctatcgt cgtttcacga
     1201 ggcgggatgg cgcgctcagg gcgtcgaggt cggcccctcg atggccggct acgcgcgcga
     1261 gcgattcggc ctggacgtcg agaccgccac gatagagtcc gccacctacg agatcggctc
     1321 cttcgacgcg gtagtcgcca cgcaccttat cgagcacctc aacgatccgc gctccttcct
     1381 ggccagggcg agggccgccc tgcgccccga aggccgactg taccttataa cgccgaacgc
     1441 cgacggttta caggccaggc tgatgggctc tcgatggcgc agcgccattc gcgatcacct
     1501 attcttgttc tcggcgagga cgcttggcgc gatgctggcg gacgagggct attacgtcga
     1561 gtacgtcggc acctggggcg gttggcccgc cggcatgcgc cccgtcagcc tgaaacggcc
     1621 catggatagg ctggccaagc ggtacggctt gggcgacgtc atggtcgtca gggcttctca
     1681 ggccacggtt ccgacgcctc tggaggtacg atgaccgacg actacaggat aaccgtcgcc
     1741 caggcggtgg agaacgtgct cgagaggatg ggagcggcct gcgccagggc cggacggtcg
     1801 gccgacgagg tacgcctact cgccgtcaca aaattccatc cctacgaggc ggtggaagcg
     1861 gcctacgccg ccggtgtcag gctattcggc gagaaccgcg ttcaggaagc cgaggccaaa
     1921 ttttccgccg gcacggctcc gaagggcgcc ttcgtccacc tgctcggcca cctccagagt
     1981 aacaaggcca agcgcgccgc ggccctgttc gactgcgttc agtcgataga ctcgatagag
     2041 gtcgccgccg agcttgccaa gcgcgccgcg gcgctcggca agaccctcga cgtgctcctt
     2101 gaactgcata ccggcgagga atccaagacc ggcttcgcga gcgaagacgc gctcctacgc
     2161 gccctggagg ccatcatgcc gatgccgtcg ctcaagcctc gcggactcat gaccatggcg
     2221 cccttcagcg atgacgccgc ggtcgtacga gcgtcgttca ggacctgccg tagggctttc
     2281 gaggtggcca tgaaacggtt cgccccggtc gatttctccg tgctgtccat gggcatgtcg
     2341 aacgatttcg agctggccgt ggaggaaggc tcgacgatgg tgcgcgtcgg cacggccatc
     2401 ttcggaagca ggcggcttcg atgaggctcg ccaggagcgt cgcggcgcta tcgctcgcgc
     2461 tcatggcggc gaccgcggcc ttcccgcagt cggcctcgac tccggctgaa tcgtcgctgc
     2521 ccgagccgta ccgagcggga gaattccccc cgtggctcga cgcggccagg cgattcgaga
     2581 tcgtcggcct cggcgtcttc cccatcctgc tcttctatac gcgcttcgcg agcgacgccg
     2641 ctcgctacgt acggaacggt ttcgacgcga cgtacgcgcc gtggccgttc aagaacgagc
     2701 aatcctacga gccgagcgac gacgagcaat ggaagaacgt attgacggcc gccggcctgt
     2761 ccgtagcgtt cgcggcgttg gacgcactcg tactctggcg cgttcagctg gagtagccga
     2821 tggcgcagcg acccggtact taacagggta ggctgacctg cattagtatt gcgggtatgc
     2881 atagacaagc ggaagcgcgc gacgcgacgg tgctggccgt cgacgtcggc gggacgaata
     2941 ccagttgcgc cgtcgtcgac gtccgtgacg gctactttac gacgactttc gagcgtcgct
     3001 attcgactaa agccgagccg tcgctgtacg agccgctcgg ccgctttatc gacgaggctt
     3061 cctccgccgg agtagccctg cccgagctcc tctgcgtctc cggcgccggc cccgtcgtcg
     3121 accgccgcat agcgcttacc aacgcgccct ggggcatcga cggggacggc ctctccgagc
     3181 gcctcgggct gccgacctac gtcatcaacg atttaagcgc catcgcctgg ggcgtgctac
     3241 tcctcgatcc aaccgacccg aacgagcttg cccagctttc ggctcccgac ggcgccgctc
     3301 ccgatccgta tggcccggtc gtggtcatcg gcgcgggtac cgggttaggc ttcggatacg
     3361 cgctacgcga cgacggatcc gtcagggtct atccgtccga gggcgggcac gtctgcctac
     3421 cggtctacga cgacgagtcg cgcgcgttct cgcgctggct cgaggctcgc tacggcttcg
     3481 cggccggcgc cgaggccggc gtctccggac agggcatcgc cgccatcttc gagttcctgt
     3541 ccgcctccga gcccagcctg tccgaagccg cgcgcgcggc cctatccgcg ccggccgaag
     3601 ataggccggc cctcgtgtcc accgccgccg actcaggcga cgcgctatgc ctacgggtta
     3661 tggatatgtt cgtgcgactg tacgcgcgag tcgcggccga cgccgcctcg gtcttcatgc
     3721 cgtcaggagg catctacctg gcaggcggca tcgccgccaa gaacctggcg cgatttaccg
     3781 agggcgatcg cttcatgcgc agcttcggcc agggctatcg ggagcacgtc cgggcgatcg
     3841 cggctaggac gccggtcttc gtcgtcaagg actacgctat atcgatgtac ggggcggcga
     3901 acgccgcggt ccacttggcc gacggatcga gatggcggcc gtgctgagcg acgcgctgcg
     3961 atctgacatg gtcgccaagg gagtcgacgt cgagacgacc ctgtccatct tggccgacct
     4021 gaacgcgggc ctctatgacg gtcagaaggc cgtgcgggct accggcgtgc cgccagtcga
     4081 cggcgcatcc atcgtgccct ccgccaagga catggcctac aggataggcg ctgacgaggc
     4141 gctgagtcgg ctctcggacc tcggcctcga gcttcccgat ggcgcgcgag ccgacggcga
     4201 cagcctgctg ttcgaccggg ccgccctcga ggacctcgga gagcggctgt acccagtcgc
     4261 cgcctggggc gtactcaacg gcggctcggc gacgagctac gccgaccgca agaagaacgc
     4321 cgccctcggc cccggcgtgt tcgaagcgat acggtcgggc ttcgagctcc tggcgcctct
     4381 atgcgacggc aggcccaagg gcgtcacccc ggcctacatc aacccggacg gtagccccgg
     4441 cgagagtttc ctgctcctga agatgcgggc ggccctcctg cgcgccgaac gctacgtcga
     4501 gcgcttcggg atgccggcgc ggccggcgct cccgttcttc cagatgacga gcgacggcac
     4561 cgacgctcag ctcgccgacg cctatgccgt gtatgc
//
LOCUS       DAOPHV010000158         4581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_25372_length_4581_cov_3.786346, whole genome shotgun sequence.
ACCESSION   DAOPHV010000158 DAOPHV010000000
VERSION     DAOPHV010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25372_length_4581_cov_3.786346"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLQ29_05475"
     CDS             complement(<1..485)
                     /locus_tag="PLQ29_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HPG86132.1"
                     /translation="MNALGTTGACSEALHLPLRGANAPVAVMSLAAAGDMGFGDDGNR
                     SRRRAWLAARGFDPGAAASVDLVHSRTVLEALEPSGQVGREADGLVTSGAGPVRVLVM
                     TVADCMPIFVYDRDSGAFGLLHSGWKGTGILAEAVRLMGRLYGSRPGSLSVAFGPCIG
                     S"
     gene            complement(482..2200)
                     /locus_tag="PLQ29_05480"
     CDS             complement(482..2200)
                     /locus_tag="PLQ29_05480"
                     /inference="COORDINATES: protein motif:HMM:NF036553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5312 family protein"
                     /protein_id="HPG86133.1"
                     /translation="MASASTFEALVLDLSPSERSEMLERLQRSCTVSTEPLFERGHLQ
                     EPRIDYRAAYSELGLVTRIIITLRSIFGGGSKEDLVKERFLKAIVKRVESTSAGLIDA
                     KRRVLQEAFYRELATLRQAARYFYDLLDRTLEKNRAPFFAFLASLEFEEIHRELSTDA
                     DPYLVAEQNSTMLDSDVRIAVLAAMESALGRIDEDKRRLMYKDVRNLHVLKSLSSFLF
                     DRMLAAFQTNVSGLKELSMYAAAEQLAELAAILCSLDQPPSARLMEAILGFELNDDVG
                     REGFDLDEAVTTELASAEKALAAIRGFNARVPIEDILKVANEDPNWQCATTGGGEDWF
                     AIFKSYWRERIDRRYQCFSAERRIAQLDSDIQALVGRDAPTWFQYLSESGGERIPPMR
                     YARALRFLEAFYHHTFLSDINKSLKLVLLEGEFYKRDNRLDFTDAYNEILQIGDGLKR
                     LDSRLSPDGELGGAYGQARTELSSAQIKRRKIDPAAKAADTEAEALLSKSADAMRRMN
                     EILKAILSRETRGRYDSLSNLGRIEGKANKEFQKSLESSKDKLEKANFLLSELIKTAV
                     GGLDQA"
     gene            complement(2230..3051)
                     /locus_tag="PLQ29_05485"
     CDS             complement(2230..3051)
                     /locus_tag="PLQ29_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPG86134.1"
                     /translation="MDTEFDPIAEPRVVYEGDGVIVVAKPSGMHCAPAGRLAPAGPGS
                     TLSDWLFERYPMAAAVAGRGRGEGGLLHRLDGATSGLVAFATDDGSFAAMSAAAARGA
                     FVKTYRALCLPAPVGLAGSRPELYAPPGADPDRWRSCLLRGDAPALASMLAGASIESR
                     FRPYGPGAARVACATIGPGPASRAKDWTRDSYLSDVVTARPFSSGLLVEVRLARGFRH
                     QIRAHMAWLGLPLEGDDLYGDGGNRAAELRLRAFRLSFPSPRGSGPVVVELTEPC"
     gene            3169..>3359
                     /locus_tag="PLQ29_05490"
     CDS             3169..>3359
                     /locus_tag="PLQ29_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86135.1"
                     /translation="MSTTTKIDAARESARRGGTRLTAPTLVASLRRVARETSRSGLVS
                     LFAAFFGAIVAFGLIVWAM"
     assembly_gap    3360..3459
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3460..4355
                     /locus_tag="PLQ29_05495"
     CDS             <3460..4355
                     /locus_tag="PLQ29_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HPG86136.1"
                     /translation="AAFFGAIVAFGLIVWAMERTDPAGGISTPIDGLWWAVVTLATVG
                     YGDTYPASALGKAAAMAFMIGGFVLSAIVSGTVASIFVERRIREGKGLQDIRLKGHFV
                     VCGWNVHADAVLDGLEAESRGAPIVLINGLEPERFDAIRSAHPDVDLRFVRGDHTLES
                     VLRKASVQQCRSCILLPDESGGSGEANADERTRLAALAVKSIARDVAVRAAILKAESE
                     QHLRRAEVDDVVLHGEFTGFLSDSSGAIDDFIKRKFAEAAIDLGSGDKPEGKARLAPG
                     PDYVIGGTDVAFVVGGSVTHG"
     gene            4348..>4581
                     /locus_tag="PLQ29_05500"
     CDS             4348..>4581
                     /locus_tag="PLQ29_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPG86137.1"
                     /translation="MAKDAKLLRRTSLLDGLSSDAVALVLASGSFVRFKAGETVMHEG
                     EKSDTMYLFLEGDVDVTKDLTLKISGKGFGHAEK"
BASE COUNT          752 a         1508 c         1500 g          721 t
ORIGIN      
        1 cacgaaccga tgcatggtcc gaaggcgacg ctcaggctcc cgggcctgga accgtagagc
       61 cgccccatga gccggaccgc ctcggccagg atgcccgtcc ccttccagcc agaatggagg
      121 agcccgaagg cgcccgagtc ccggtcgtaa acgaagatcg gcatgcagtc ggcgaccgtc
      181 atcacgagga cgcggacggg accggcaccg ctagtcacga ggccgtccgc ctcgcggccg
      241 acttggcccg acggctcgag ggcctcgagc accgtcctcg agtgcaccaa gtcgaccgac
      301 gcggccgccc cgggatcgaa gccccgcgcg gccagccagg ctcgtcgcct cgaacggttg
      361 ccgtcgtcgc cgaagcccat gtcgcctgcg gccgccaggc tcataacggc aaccggggcg
      421 ttcgcgccgc ggagcggtag gtgtagcgcc tcggagcagg ctccggtcgt cccgagtgcg
      481 ttcacgcttg atccaggccg ccgacagccg tcttgatcag ttccgaaagc aggaagttcg
      541 ccttctcgag cttgtccttg gaagattcaa ggctcttctg gaactccttg ttcgccttgc
      601 cctcgatgcg gccgaggttc gatagcgagt cgtagcgccc gcgggtctcc cgggagagga
      661 tggctttgag gatctcattc atgcgcctca tcgcgtccgc cgacttggac agcaaggcct
      721 ccgcctcggt atccgccgcc ttggcggcgg ggtctatctt acggcgcttg atctgcgccg
      781 acgacagctc cgtgcgcgcc tggccgtagg cgccgccgag ctccccgtcc ggcgacagcc
      841 tcgagtccag gcgtttgaga ccatcgccaa tctgcaggat ctcgttgtac gcgtcggtga
      901 agtcgagcct attgtcgcgc ttgtagaatt caccctcgag cagcaccagc ttgagcgact
      961 tgttgatgtc gctcaggaag gtatgatggt agaacgcctc gaggaagcgt agcgccctcg
     1021 cgtagcgcat cggcgggatc cgctcgccgc cggactcgga caggtactgg aaccaggtcg
     1081 gggcgtccct gccgacgagg gcctggatat cggaatcgag ctgggcgata cgccgctccg
     1141 cggagaagca ttggtatcgg cggtcgatcc gctcgcgcca atacgacttg aagatggcga
     1201 accagtcctc gccgccgccc gtggtcgcgc actgccagtt cgggtcttcg ttggcgacct
     1261 tgaggatgtc ctcgatgggc accctcgcgt tgaagccgcg aatggccgcg agggcctttt
     1321 cggcgctggc gagctcggtt gtaaccgcct cgtcgaggtc gaagccctcc cggccaacgt
     1381 cgtcgttcag ctcgaagcct aggatcgctt ccatcaaccg ggccgagggc ggctggtcca
     1441 gcgagcagag tatcgccgcg agctccgcga gctgctcggc ggccgcgtac atcgacagct
     1501 ccttgagccc ggagacattg gtctgaaacg ccgcgagcat gcgatcgaac aggaagctcg
     1561 aaaggctctt gagcacgtgg agattgcgca cgtccttgta catcaggcgg cgcttatcct
     1621 catcgatgcg gccgagcgcg gactccatcg ccgcgaggac cgctatgcgc acgtcggagt
     1681 cgagcatggt cgagttctgc tcggcgacga gatacgggtc cgcgtcggtc gatagctccc
     1741 ggtgtatctc ctcgaactcc agggacgcga ggaacgcgaa gaacggcgct cggttcttct
     1801 cgagagtgcg atcgaggagg tcgtagaagt acctggcagc ctgcctgagg gtcgcgagct
     1861 cccgatagaa ggcttcctgg agcacgcgcc gcttcgcgtc gatcaacccc gcgctcgtcg
     1921 actcgacgcg tttgactatg gccttgagga agcgttcctt gacgaggtct tccttgctgc
     1981 cgccgccgaa gatcgaccgc agggtgataa tgatgcgcgt caccaagccc agctcactat
     2041 aggcggcccg gtaatcgatg cgcggctcct ggaggtgtcc ccgctcgaac agcggctcgg
     2101 tcgagaccgt gcacgagcgc tggagccgct cgagcatctc cgagcgctcc gaggggctga
     2161 gatcgaggac gagggcttcg aacgtcgatg cgctggccat atccgtaatc ctatccggcg
     2221 aatggcgtat tagcaaggct ccgttagctc gacgacgacg ggccccgagc ctcggggcga
     2281 cgggaacgac agcctgaagg cccggagccg tagctcggcg gcccggttcc cgccgtcgcc
     2341 gtataggtcg tcgccctcga gcggcagtcc aagccaggcc atatgcgcgc ggatctggtg
     2401 cctgaagccg cgcgcgagcc gtacctcgac caggagcccc gacgagaacg gccgagcggt
     2461 tacgacgtcg ctgaggtacg agtcgcgagt ccagtccttg gcgcgagacg ctggcccggg
     2521 cccgatcgtc gcgcaggcga cgcgggccgc tcccgggcca tagggcctga agcggctctc
     2581 gatcgacgcg ccggccagca tcgacgccag ggccggggcg tcgcctcgga gcaggcagga
     2641 gcgccaacgg tcaggatcgg ctccaggcgg cgcgtacagc tccggccgcg aaccggcgag
     2701 gccgaccgga gccgggaggc atagcgcgcg ataggtcttg acgaaggcgc cgcgcgccgc
     2761 cgcggcgctc atcgcggcga agctcccgtc gtcggtcgcg aacgccacga gacccgaggt
     2821 ggcgccgtcg agccgatgca gtagcccacc ctcgccccta ccccggcccg ccaccgcggc
     2881 cgccatggga tagcgctcga acagccagtc gctcagggta gagccagggc cggccggagc
     2941 gagccgcccg gccggagcgc agtgcatgcc ggatggcttg gcgaccacga tcacaccgtc
     3001 gccttcgtag accacgcgcg gctccgctat aggatcgaac tctgtatcca cgcgacctcc
     3061 gagaacggaa gcctatacgc gtctccgggg taatgcaagg gacccgcgcc gcacctgccg
     3121 acatggcggc tcatcgggca acggcctcct gccgatattg aggaggtgat gagtaccacc
     3181 acgaagatcg acgcggcgcg cgaatcggcc aggcgcggcg ggacgcggct cacggcgcct
     3241 accctcgtag cgtcgctacg ccgcgtcgcc agggagacga gccgctccgg cctcgtctcc
     3301 ctgttcgccg ccttcttcgg cgccatcgtc gccttcggcc tgatcgtatg ggccatggan
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cgccgccttc ttcggcgcca
     3481 tcgtcgcctt cggcctgatc gtatgggcca tggagcgcac cgatccggcc ggcggcatct
     3541 cgacaccgat tgacggtctg tggtgggcgg tcgtgacgct ggcgaccgta ggctatggag
     3601 acacgtaccc ggcgagcgcc ctcggcaagg cggcggcgat ggcgttcatg ataggcggct
     3661 tcgtgctgag cgccatcgtc tcgggtaccg tggcctcgat cttcgtcgag cgccgcatca
     3721 gggagggcaa gggcttgcag gacatacggc tcaaggggca tttcgtcgtc tgtggctgga
     3781 acgtccatgc cgacgcggtg ctcgatggac tcgaggccga gtctcgcggc gcgcccatag
     3841 tgctgatcaa cgggctcgag cccgagcgct tcgacgccat caggtcggcc cacccggacg
     3901 tggacctcag gttcgtccgc ggcgaccata ccctggagtc ggtgctccgc aaagcctcgg
     3961 tccagcagtg ccgctcgtgc atactcctgc ccgacgaatc gggcggttcc ggggaagcca
     4021 acgccgacga acgcacgaga ctcgcggccc tggccgtcaa gtcgatagcc agggacgtcg
     4081 ccgtgcgcgc cgccatactc aaggcggaga gcgagcagca cctcaggcgg gccgaggtcg
     4141 acgacgtggt gctccacggg gagttcaccg gcttcctctc cgacagctcc ggcgccatcg
     4201 acgacttcat caagcgcaaa ttcgccgagg cggcgataga tctaggctct ggcgacaagc
     4261 ccgagggcaa ggcccgcctc gcgccaggcc ccgactacgt gatcggcggc accgacgtgg
     4321 ccttcgtcgt cggcggaagc gtcacccatg gctaaggacg ccaaactgct gcgcaggacc
     4381 tcactcctcg acggactatc ctccgacgct gtcgccctcg tactggcctc cggtagcttc
     4441 gtgcgcttca aggcgggcga gacggtgatg cacgagggcg agaaatcgga taccatgtac
     4501 ctgttcctcg agggcgacgt cgacgtgacg aaggatctga cgctcaagat ctccggcaag
     4561 ggcttcggcc acgcggagaa a
//
LOCUS       DAOPHV010000159         4558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_25554_length_4558_cov_4.944259, whole genome shotgun sequence.
ACCESSION   DAOPHV010000159 DAOPHV010000000
VERSION     DAOPHV010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25554_length_4558_cov_4.944259"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PLQ29_05505"
     CDS             complement(<1..647)
                     /locus_tag="PLQ29_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPG86138.1"
                     /translation="MRKRNIVAAAVVALVLASCGGEKQADVIKVGWFGALTGDQAVWG
                     NNEFNTVKMLFEEINAAGGVTVGGKAYTLEAVGYDNKGDAQEAVNVVRRLTSQDKVVA
                     ILGPNASGNAIPIAPILESAKVPDIATVATNPKVTVLDGKVKPYNFRVCFIDPYQGAV
                     AAGYAFDVLGARKAAVLYDVSDDYSQGLREFFIQNFEKKGGTIVADESFKSGDVDR"
     gene            825..2300
                     /locus_tag="PLQ29_05510"
     CDS             825..2300
                     /locus_tag="PLQ29_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86139.1"
                     /translation="MNETMIFGNELPKRALRKADRSLKSMARRFSFDPAVYPRLSAEP
                     MPGAYEAFGVMKLEEAKPGATVVPPDPGKGIALGTIRMGFGHYRIGLAIASAARSMGL
                     TPYWLDLMSFPDSAASKTIKYLEDLYNLGSRLSQRSRLFDRYVWDRITSSAARRLSYT
                     ARDRALSTLFEPILRDLPKDMPFMSTHPWTGQAAVRAGMKGVVALIPDNYPLAFHLVE
                     GAVHTVQAPSLYMGYRTLRDMGGGEELFHALPKGDIRCVGHYVDHEIVSGVEEDCAAR
                     LRRLKDRGTRRFLLTMGGAGAQARRFADIAHACKGAIEAGKVSLLINMGDHAGRWAEL
                     KGWLDRDGIPYEMHGDWVGTKAFAEEARTAPVKGVHVFLHDNFFAAVYTTNILMRVVD
                     LMITKPSELSVYPVPKLFIQHVGRHEVWGAIRGSELGDGTLETESDASLRQALRLLVE
                     EDDLLEMYCGNILKNYRAGVYDGAYGAVHLALERRSDGAGR"
     gene            2424..2948
                     /locus_tag="PLQ29_05515"
     CDS             2424..2948
                     /locus_tag="PLQ29_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HPG86140.1"
                     /translation="MDKYRFTYQAIHRTVRDLADRVLAAGYETDIIVAIGSGGFIPAR
                     MLRTFINKPILAVGIAYYDQDNQPTDNPRKVQWIEEAERQLKGKRILLVDEVDDSRVT
                     LEYCVRELLSHHPESVAVAVLHNKLKEKRGAMPPEVKLYLSGLDIEDKWVCYPWDAQD
                     IDRHDRDAATTGQA"
     gene            2945..3583
                     /locus_tag="PLQ29_05520"
     CDS             2945..3583
                     /locus_tag="PLQ29_05520"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPG86141.1"
                     /translation="MIAPDEGRRDVAALKAALRKSAKAALAAVTPERRAVASADAAAR
                     ASAHPAWPGAELVLAFLSMPSEIDTAPVVEAALAAGKRVAVPRIDGGDIAFVELAPGW
                     RAWPRDRWDIPAPPAETPSIPPAEIAGLATLALVPGLAFDRTGGRLGRGKGYYDRFLA
                     SVAAARSAAAARSSGPSSPFVAIGYGYESQIVDSVPTDERDLPLDGLSLGQA"
     gene            3635..4111
                     /locus_tag="PLQ29_05525"
     CDS             3635..4111
                     /locus_tag="PLQ29_05525"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HPG86142.1"
                     /translation="MQAKKRVALVAHDNRKLDLVGWVEYNWRSLLKHELVCTGTTGRL
                     VSETIERKRSTEGGAGAPASSITLLKSGPLGGDQQLGAMIAEGRIDAVIFFWDPMSPQ
                     PHDVDVKALLRIAVVYNIPMACNRSTADFMISSPLFERAYDPIVKDYSAYIQRKVD"
     gene            complement(4108..>4558)
                     /locus_tag="PLQ29_05530"
     CDS             complement(4108..>4558)
                     /locus_tag="PLQ29_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86143.1"
                     /translation="ARLKGLGYRRLGLSVSLNAPDDGLRSTLMPVGGAHGLGELAAAL
                     AAYPSRRNFALGVNYCLIPGVNDGPGAADGVAAFVRAAGRALVNVIPYNPGTAPIGRA
                     PTEPEVDAFMASLTERGLEARVRAPRGRGLAAACGQLGGPNATVGVP"
BASE COUNT          787 a         1563 c         1478 g          730 t
ORIGIN      
        1 cggtcgacgt cgccggactt gaacgactcg tcggctacga tcgtgccgcc cttcttctca
       61 aaattctgga taaaaaactc gcggaggccc tgcgagtagt cgtcggacac atcgtagagc
      121 accgcggcct tgcgggcgcc gagcacgtcg aacgcgtagc cggccgcgac agcgccctgg
      181 taggggtcga tgaagcagac gcggaagtta tacggcttga ccttgccgtc gagcaccgtg
      241 accttcgggt tggtcgcgac ggtggcgatg tccggtacct tggcgctctc gaggattggc
      301 gctatcggaa tggcgttgcc ggaggcgttc gggccgagga tagccacgac cttatcctgg
      361 ctggtcaggc gcctaaccac gttcaccgct tcctgagcgt cgcccttgtt gtcgtagccc
      421 acggcctcga gcgtgtaggc cttaccgccc accgtcacgc cgccggcggc gttgatctcc
      481 tcgaagagca tcttgaccgt gttgaattcg ttgttgcccc agaccgcttg gtcgccggtg
      541 agggctccga accagccgac cttgatgacg tcggcctgct tctcgccgcc gcacgacgcg
      601 aggacgaggg cgactacggc cgccgccacg atgttccgtt tgcgcatggt gcctccaaaa
      661 atagatgttt ctacctatcg ctataatcgc tcattcgtca aggtcgcatt tttatacaac
      721 tcttctgtat atttatgcaa gccctccggg tagacaacag gcaggcttgc caaacggctc
      781 ggcgcgtata tagtcgaggt agtggatgat gcacggaggt tcgcatgaac gagacgatga
      841 tcttcggaaa cgagctaccc aagcgcgcgc tcaggaaggc cgaccgatcg ctcaagtcga
      901 tggccaggcg cttctcgttc gacccggccg tctatccccg actgagcgcc gagccgatgc
      961 ctggcgccta cgaggcgttc ggcgtgatga agctcgagga agcgaagccc ggcgcgaccg
     1021 tcgtcccgcc cgaccccgga aagggcatag cgctcggcac catacgcatg ggcttcggcc
     1081 actaccgcat cggcctggcc atagcctcgg ccgcgcgatc gatgggccta acgccatact
     1141 ggctcgacct gatgtccttc ccggacagcg ccgcgagcaa gaccatcaag tacctcgagg
     1201 acctgtacaa cctcggctcc aggctgtcgc aacgctccag gctgttcgac cgctacgtct
     1261 gggatcgcat cacctcgagc gcggccaggc gtttgtcgta cacggcgcgg gaccgggcgc
     1321 tctcgactct gttcgagcct atcctgcgcg acctgcccaa ggacatgccc ttcatgtcga
     1381 cccacccctg gaccggccag gccgccgtcc gcgccggcat gaagggcgtc gtcgcgctga
     1441 taccggacaa ctacccgctc gcgttccatc tggtcgaggg cgccgtgcat acggtccagg
     1501 cgccatcgct gtacatgggc taccgcacgc tgcgtgacat gggcggcggc gaggagctgt
     1561 tccatgctct gccgaagggc gacatacgct gcgtcggcca ctacgtcgac cacgagatag
     1621 tctcgggcgt cgaggaggac tgcgccgcga ggctcaggcg cctcaaggac cgcgggacca
     1681 ggcgcttcct cctgacgatg ggcggcgccg gagcccaggc caggcggttc gccgatatag
     1741 cccacgcctg caagggcgcc atcgaggccg gcaaggtatc gctactcata aacatgggcg
     1801 accacgcggg gcgctgggcc gagctcaagg gctggctcga ccgggacggc attccgtacg
     1861 agatgcacgg cgactgggtg ggcaccaagg ccttcgcgga ggaggcgagg acagctccgg
     1921 tcaagggcgt gcatgtcttc ctgcacgaca acttcttcgc ggccgtctat accacgaaca
     1981 tcctgatgcg cgtcgtcgac ctgatgatca ccaagccgag cgagctgtcg gtctacccgg
     2041 tgcccaagct gttcatccaa cacgtcggcc gtcacgaggt ctggggcgcc atacgcggct
     2101 cggagctcgg cgacggtacg ctcgagaccg agtcggacgc cagcctcagg caggcgctca
     2161 ggctccttgt cgaggaggac gacctcctgg agatgtactg cggcaacata cttaaaaact
     2221 accgcgccgg cgtctacgac ggggcctacg gcgcggtgca cctcgcgctg gaacggcgat
     2281 cggacggggc tgggcggtag cggcacagca tggcgcggcc gaggcggccg aggcttctag
     2341 ctccagcagt tccttggtgg agctcgatac gccctcgtca tgactatctt gcgcccgacc
     2401 gcttgacggg cgtatgatcg gacatggaca agtaccgatt cacctaccag gccatccacc
     2461 gcacggtacg cgacctagcg gatcgcgtcc tggcggccgg atacgagacg gacatcatcg
     2521 tcgccatagg ctcgggcggc ttcatcccgg cccgcatgtt gcgcaccttc atcaataagc
     2581 ccatcctcgc cgtcggcatc gcctactacg accaggataa ccagccgacg gacaacccgc
     2641 gcaaggtgca gtggatcgag gaagccgagc gccagctcaa gggtaagcgc atcctgctcg
     2701 tcgacgaggt cgacgactcc cgcgtcaccc tcgagtactg cgtgcgcgag ctcctatccc
     2761 atcatcccga gtcggtggcc gtggcagtac tgcacaacaa gctcaaggag aagcgcggcg
     2821 ccatgccgcc cgaggtcaag ctctacctat ccggcctcga catcgaggat aagtgggtct
     2881 gctatccctg ggacgcccag gacatagacc ggcacgaccg cgacgcggcg actaccggac
     2941 aggcatgatc gccccggacg aggggcgacg cgacgtcgcc gcgctcaagg ccgcgctccg
     3001 aaagtcggcc aaggccgccc tcgcggccgt gacgcccgaa cggcgagcgg tagcgagcgc
     3061 cgacgcggcc gcccgggcta gcgcccaccc cgcgtggccg ggagcggagc tcgtgctcgc
     3121 cttcctatcg atgccgtcgg agatcgatac cgccccggtc gtcgaggccg cgctcgccgc
     3181 gggcaagcgg gtagccgtgc cgcgcatcga cggcggcgac attgccttcg tcgaactcgc
     3241 gccgggctgg cgggcctggc cgcgcgaccg ctgggacatc cccgcgccgc cggccgaaac
     3301 tccgtccata ccgccggccg agatcgccgg gctggcgacg ctggcgctcg tgccagggct
     3361 ggccttcgac cggaccggcg gacgcctggg gcgaggcaag ggctactatg accgcttcct
     3421 ggcttctgtg gcagcggcgc ggtctgcggc cgcggcgcgg tcgtccggcc cctcgtcgcc
     3481 cttcgtcgcg ataggctacg gctacgagtc ccaaatcgtc gattcggtgc cgaccgacga
     3541 acgcgacctg ccgctcgacg gactatcgct cggccaggcg taggcccgga gccggccgtt
     3601 ccatcgttct agcgaacctg aagaggagat atcgatgcaa gccaagaaac gcgtggcgct
     3661 ggtggcccac gacaaccgca agctggatct ggtcggctgg gtcgagtaca actggcgatc
     3721 gttgctcaag cacgaactcg tctgtaccgg cacgaccggc cgcctggtat cggagacgat
     3781 agagcgcaag cgctcgaccg agggcggcgc cggggctccg gcgtcgagca tcaccctgct
     3841 caaatccggc ccgctcggcg gcgaccagca gctcggcgcg atgatagcgg agggccggat
     3901 cgacgcggtg atcttcttct gggacccgat gtcgccccag ccgcacgacg tggacgtcaa
     3961 ggcgctcctg cgcatcgcgg tggtctacaa catcccgatg gcctgcaacc gctccaccgc
     4021 cgacttcatg atctccagcc cgctgttcga gcgggcctac gatcctatcg tcaaggacta
     4081 tagcgcctat atacagagga aggtcgacta gggaacgccc acggtcgcgt tcgggccgcc
     4141 gagctggccg caggcggccg ccaggccgcg accccgcggc gcccgaacgc gcgcctccag
     4201 gccgcgttca gtcagcgagg ccatgaacgc gtccacctcc ggctccgtgg gggcccggcc
     4261 tatcggcgcc gttcccggat tgtacggtat cacgttgacg agcgcccgcc ccgcggcccg
     4321 gacgaaggcc gcgacgccgt cggccgcccc cggcccgtcg ttgacgcccg gaatcaggca
     4381 gtagttgacc ccgagcgcga agttgcgcct ggacggatag gcggccaggg ccgcggcgag
     4441 ctcaccgagg ccgtgagccc cgccgaccgg catcagcgtc gatcgcaacc catcgtccgg
     4501 cgcgttcagg ctgaccgata ggccgagcct tcggtacccg aggcccttga gccgagcg
//
LOCUS       DAOPHV010000160         4547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_25648_length_4547_cov_4.259795, whole genome shotgun sequence.
ACCESSION   DAOPHV010000160 DAOPHV010000000
VERSION     DAOPHV010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25648_length_4547_cov_4.259795"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..976)
                     /locus_tag="PLQ29_05535"
     CDS             complement(167..976)
                     /locus_tag="PLQ29_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HPG86144.1"
                     /translation="MADFLADSDFELYRKLIYDESGIHFSATNRSILESRLRERLREN
                     KLQTPQEYYAILVKDKEELKVLLDSVTTNLTRFFRNQAHFDAIEHYVVPALLKLRQAE
                     RKIRVWSAGCSTGEEPYTIALLLKELLPAGWKAEITASDISLKSLMVGKEGFYPETRI
                     QGIPEPYLPKYFERRGNGYQVKDEIKNSIRFDYHNLKNDSGQRGLDVVFCRNVLIYFD
                     EAAQKATIERFWEAMAPKSFLFIGHSESLFGMNTKFEFVKTDWACFYKKDI"
     gene            1117..2616
                     /locus_tag="PLQ29_05540"
     CDS             1117..2616
                     /locus_tag="PLQ29_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPG86145.1"
                     /translation="MKNPNDRGRDRAAWGVVAVVSAFCVALSFSAPSAYAQDSKAESQ
                     RYFSIFQSAFNFILENYVEEVDPKVLYEGAMQGMFDSLGDPYSSFLTDAMMSDLNDTT
                     TGRFGGIGLYISKPLADAKAPDRRLFVEVVSPIEDTPGWRAGIMPGDQITHIDGEPTE
                     PLTMDEVLGRLRGEPGSTVTITLLRGAGLVFDERITRAVIEVPTVKRAMLPGGAGYLR
                     IIEFTPQTVSRVSEAIEWFKSQRYTSLVIDLRNNPGGLLQSVIQIADLFLDRGVIVST
                     KSRNRYENAVYQAKPPLAVPKDLRVVVLINRGSASASEILAGALKDQKRAYLIGEKSY
                     GKGSVQQVLPIGETGFKLTMSRYYTPSDANIDKVGIAPDLEVKEPELSPEEEESLSGL
                     IERDVFAAFAAANPNASPQQVDQYVEGLRAAGSPVSERVLKKLVRNQLERTSISPVYD
                     LEYDLQLNAALDALDDDDFWSRISEAYSVMELQALAAGAQAAGAVTAGR"
     gene            2624..3469
                     /locus_tag="PLQ29_05545"
     CDS             2624..3469
                     /locus_tag="PLQ29_05545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPG86146.1"
                     /translation="MRQFVLPGSWDGGPVCELEGRDARRLLTVLRLSPGDSFPAIGPD
                     GAPYSCRIESAGRGRLTLSVAPGALASAGYKPDVRAGRPASLVAGLAAGPSRDEAVAV
                     PRLILAVGLLKGSKLDEVARIAAEAGVEAIVPLCTARALPRDHADGRIERLRRVVAEA
                     IGQSGSGVATRVEAPTTVAGLCVARPGRSPGRLGLFFHEAPLAQSSIHRYCTDVPDEI
                     VACVGPEGGFDDTEVGALSDSGYKAAWLGPTVLRAETAAIFAVASVRIVCLERPSWST
                     TESRE"
     gene            3442..4188
                     /locus_tag="PLQ29_05550"
     CDS             3442..4188
                     /locus_tag="PLQ29_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HPG86147.1"
                     /translation="MVHDRVTRISVLKVPVDIVEPDDLEEVVKAMYSDGKNHQIVLLS
                     AADLMRARRSGEFRTMIAGASLVVPISMSIVRAAKFLKRRAPVRYEPFDFIVRLLSIL
                     ERWGKSAYLFGGSPKGLARASKNIKATFPGLRVVGGHSSRFPKHFHPKIVEAIRKAAP
                     TLLLVGRGVPGAERWIPRNMRNFNAGLQLWCSDVFEVFAERRNRPPSAMFSKGLEWLY
                     YLPRRPWIVFRLWTQLRMKVISLWYRIRGK"
     gene            4258..>4547
                     /locus_tag="PLQ29_05555"
     CDS             4258..>4547
                     /locus_tag="PLQ29_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86148.1"
                     /translation="MKRLVVVAIALAAVSCTTRPASLPEAGWEATAPAQVRKEYQREV
                     KVPVVVKETVSYADGVVDRIVTHEYDEGLMYLLASYSSKPSSLALASRVSYG"
BASE COUNT          798 a         1433 c         1450 g          866 t
ORIGIN      
        1 ggcatcgggg tatcttctgc agcgcgaaca gcccgttcat cgccttctcg gcggtacgga
       61 gccctggcgt cgagtcgact atcctggaga tgaggttcct catcaaggcc gagtcgtcga
      121 ctacgagtac ggaaatagga tcgggcacgt cattcctccg cgtcggctag atgtctttct
      181 tgtagaagca ggcccaatcg gtcttgacga actcgaattt cgtgttcatg ccgaagagcg
      241 actcggaatg cccgatgaac aggaaggatt tcggggccat cgcttcccag aaccgctcga
      301 tcgtggcctt ctgggccgcc tcatcgaaat agatcagcac gttgcggcag aataccacgt
      361 cgaggcccct ctgtcccgag tcgttcttca ggttgtggta gtcgaagcgt atcgagtttt
      421 tgatctcatc tttgacttgg tagccgttgc cgcgccgctc gaaatacttg ggcaggtacg
      481 gctcggggat gccctggatc ctcgtctcgg ggtaaaagcc ctccttgccg accatcaggg
      541 acttgaggct gatgtcgctt gccgtgatct ccgccttcca gcccgcgggc agtagctcct
      601 tgaggagcag cgcgatggta tacggctcct ctccagtcga gcaaccggcg ctccagaccc
      661 gtatcttgcg ctcggcttgg cgcagcttga ggagggcggg aaccacgtag tgctcgatgg
      721 cgtcgaaatg agcctggttg cggaaaaagc gggtcaggtt ggtcgttacc gagtcgagga
      781 gcaccttgag ctcctcttta tccttgacta ggatggcgta gtattcctgc ggcgtctgca
      841 gcttgttctc gcggaggcgc tcgcggagcc ggctctcgag gatggaacgg ttcgtcgcgg
      901 agaagtgtat gccgctctcg tcgtatatca gcttgcgata cagctcgaaa tctgaatcag
      961 ccaggaaatc cgccatcgct tgcctctccg ccgaacgtgc atggagagcg catggccctc
     1021 gcttcgtcga agtctagtgc cgagaattct aggctgtcaa tagaagggcc ttggaaggcg
     1081 gttgactgtc ggcccgccaa agaggatagt tacgctatga agaacccgaa tgatcgaggt
     1141 cgcgaccggg ccgcttgggg cgtcgtcgcg gtagtctcag cgttctgcgt cgctctgtcc
     1201 tttagcgcgc cgtccgccta tgcccaggat tccaaggccg agagccagcg atatttctcc
     1261 atattccaga gcgcgttcaa cttcatcctc gagaactatg tcgaggaggt cgatccgaag
     1321 gtcctgtacg agggcgccat gcagggcatg ttcgactcgc tcggggaccc ctactcgtcg
     1381 ttcctgacgg acgcgatgat gagcgacctg aacgatacga ccaccggcag gttcggcggt
     1441 ataggcctct atatttcgaa gcccctggcc gacgccaagg ctccagaccg tcggctattc
     1501 gtcgaggtcg tctcgcccat cgaggacacg cccggctggc gcgcaggcat catgcccggc
     1561 gaccagatca ctcatataga cggtgagccg accgagccgc tgactatgga cgaggtgctg
     1621 ggccggctac gcggcgagcc gggctcgacg gtgaccatta ccctgctccg tggcgccggc
     1681 ctcgtgttcg acgagaggat caccagggcg gtcatcgagg tgcccacggt caagcgggcg
     1741 atgctacccg gcggcgccgg ctacctgcgc atcatagagt tcaccccgca gaccgtctcc
     1801 agggtcagcg aggctatcga gtggttcaaa tcgcagcggt atacgtcact cgtcatcgac
     1861 ctgcgcaaca accccggcgg cctgctccag tcagtcatcc agatagcgga cctgttcctc
     1921 gaccgcggag taatcgtgtc gaccaagtcg cgcaaccgct acgagaacgc cgtttaccag
     1981 gccaagccac ccctggcggt gcccaaggat ctgcgcgtcg tcgtgctgat caatcgcggt
     2041 tcggcgagcg cctcggagat cctggccggc gccctcaagg accagaagcg cgcctacctg
     2101 ataggggaga agagctacgg caagggctcg gtgcagcagg tgttgccgat cggcgagacc
     2161 ggcttcaagc tgaccatgtc caggtactac acgccgagcg acgcgaacat cgacaaggtc
     2221 ggcatcgcgc ctgacctcga ggtcaaggag ccggaactca gccccgagga ggaagagtcc
     2281 ctgtccggcc tgatcgagcg cgacgtcttc gcggccttcg cggcggccaa cccgaacgcc
     2341 tcgccacagc aggtcgacca gtacgtcgag gggctccgcg ccgcgggctc accggtgtcc
     2401 gagcgcgtgc tcaagaagct ggtacggaac cagctcgagc gaacgtcgat cagcccggtc
     2461 tacgatctcg agtacgacct gcagctgaac gcagcgctcg acgcgctcga cgacgatgat
     2521 ttctggtcgc gcatatcgga agcgtactcg gtgatggagc tacaggctct ggccgccggc
     2581 gcccaggcgg ccggcgcggt caccgcgggc cgctgaccag gggatgcgcc agttcgtcct
     2641 gcccgggagc tgggacggcg gtcccgtctg cgagctcgag ggccgcgatg cgcggcgcct
     2701 cctgaccgtg ctcagactgt ctcccggcga ctcgttcccg gccatcggcc cggacggcgc
     2761 gccgtactcg tgccggatcg agtccgccgg gcgcggtcgg ctgaccctgt cggtcgctcc
     2821 cggggccctc gcatccgcgg gctacaagcc ggatgtcagg gccggtcggc cggcaagtct
     2881 cgtggcaggt ctcgcggccg gtccgagccg tgacgaggcc gtagccgtac cgcggttgat
     2941 cctggccgtc ggcctgctca agggctccaa gctcgacgag gtggccagga tagccgccga
     3001 ggcaggagtc gaggccatcg tacccttatg caccgcgagg gcgcttccgc gcgaccatgc
     3061 cgacggccgt atcgagaggc tcaggcgcgt ggtggccgaa gctatcggcc agtccggttc
     3121 cggcgtggcc acgcgcgtcg aggcgccgac gaccgtcgcg ggtttatgcg tggccaggcc
     3181 gggtcgatcg ccgggccggc tcgggctgtt cttccacgaa gctccccttg cgcagtcgtc
     3241 gatccatcgc tattgtaccg acgtgccgga tgagatcgtc gcgtgcgtcg ggcccgaggg
     3301 cggattcgac gacacggagg tcggggcgtt gtccgatagc ggctacaagg ccgcctggct
     3361 cggcccgacg gttctgcgcg ccgagaccgc cgcgatcttc gccgtcgcgt cagtacgaat
     3421 cgtctgcttg gagcgtccct catggtccac gaccgagtca cgagaataag cgtcctgaag
     3481 gtgccggtcg acatcgtcga gccggacgac ctcgaggaag tcgtcaaggc gatgtactct
     3541 gacggcaaga accaccagat agtcctgtta tcggccgccg acctgatgcg ggcgcgccga
     3601 tcgggcgaat tcaggacgat gatagccggt gccagcctcg tcgtccccat atcgatgtcc
     3661 atcgtacggg cggcaaaatt cctcaagcgt cgagcgccgg tgcgctacga gcccttcgac
     3721 ttcatcgtca ggctcctgtc catcctcgag cgctggggca agtcggctta cctttttggc
     3781 ggtagcccca aaggcctagc gcgcgcctcc aagaacatca aggccacctt ccccggcctg
     3841 cgcgtggtgg gcggtcattc atcgaggttc ccgaagcatt tccaccctaa aatcgtcgag
     3901 gccatacgca aggccgcccc gactctgttg ctcgtcgggc ggggtgtgcc tggcgccgag
     3961 cgctggatac cccgtaacat gaggaacttc aacgccggcc tgcagctctg gtgctccgac
     4021 gtgttcgagg tcttcgccga gcgtcgtaac cgtcctccct ccgccatgtt ctccaagggc
     4081 ttggagtggt tgtactacct gcctaggcgc ccatggatcg tattcaggct ttggacccag
     4141 ctcaggatga aggtcatcag cctctggtac cgcatccgcg ggaaatagtc ttttttctat
     4201 acttccaaca ctaaaagtcg gctatatcat ctcagtttcc actgatcgga ggaacgaatg
     4261 aagcgattag tcgtggtcgc gatcgccctg gcggcggtct cgtgcactac gcggccagcc
     4321 tcgctaccgg aagccgggtg ggaagcgacg gccccggctc aggtgcgcaa ggaatatcag
     4381 cgagaggtca aggtccccgt cgtcgtcaag gagacggtat cgtatgcgga cggcgtcgtc
     4441 gataggatcg tgactcacga gtacgacgag gggctgatgt atctcctcgc ctcgtacagc
     4501 tccaagccgt cgtcgctggc gctcgcgagc cgcgtttcct acgggta
//
LOCUS       DAOPHV010000161         4537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_25748_length_4537_cov_4.687416, whole genome shotgun sequence.
ACCESSION   DAOPHV010000161 DAOPHV010000000
VERSION     DAOPHV010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25748_length_4537_cov_4.687416"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="PLQ29_05560"
     CDS             <1..1244
                     /locus_tag="PLQ29_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HPG86149.1"
                     /translation="EVRAKIAYYKDLLAHPEKILGVIKDETKVISDKFGDDRRTEIVH
                     DEVEAINIEDLITKEEMVIMLSNKGFVKRVPVSAYRSQSRGGKGSNSASLLDDDYVQQ
                     IFVANTHDYLMYISSAGKAYWQKVHEIPEASRAARGGHLKSLLAIGANEEITTVIDLK
                     GFSDDTYLMLGTLRGVVKRVATSQFVNAKTRGIIAIHLDEGDRLVSACRTRGNDEVVL
                     ITRLGQALRISERDVRVMGRGSRGVCGIKLADADELNAMLRVNPEEQMLLISETGYGK
                     RTDYDNFNAHGRATRGQRIYDPDEKSGEIVCAITVKENDDVVVMTSLGKTLKTRAADV
                     RLCGKTARGVHIVNIDSPDFVIGMDRIINEEAEIAVSKQAHDDDAKNRESEDEPVDTV
                     AYDDEPEELPPDADDGEVLDD"
     gene            1926..2843
                     /locus_tag="PLQ29_05565"
     CDS             1926..2843
                     /locus_tag="PLQ29_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPG86150.1"
                     /translation="MTTDGKRRLKGILLVNVATCAWATNIMIGRLVRDQIGPLTLTAT
                     RYVVASIVFAFVLRARPIQERRLGKDWLLLGAMSLTGVVLFAPTLYFGLSYTQAINGT
                     LINGIGPLLTAAFAAWLLREPFSGRQLFGSVLALGGVVVLLFGSASGPSKALSLNRGD
                     LLIVVAVTFWALYSVATRRVVRDRSPISATALSMFIGLPVLLVFAVLEHGLQPVAWTP
                     RLIGLVVYVGLVPAALGFLCWNAGVKLLGAGGAMVFYNTLPLYGVLFGFLLLGETLGL
                     AHLAGGALILGGGLVAAISKRASHLAVNK"
     gene            2871..3572
                     /locus_tag="PLQ29_05570"
     CDS             2871..3572
                     /locus_tag="PLQ29_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPG86151.1"
                     /translation="MANRFIRGIDDGDITTEGELRSAFRAQALATHPDLGGGNDGSAA
                     GNDFMRVRTEYEAAVRYLARPAGDQRGALSRSAASAGFDRGLFYADLAILIKAGFPKR
                     PRHDQERRKYSRLRLNLRSSLSRLAAETLADPLGAFDAFEAALFSLRDSGNDEATVIV
                     DRVRSLLEDMIDYEACGLTPLRAAIAIEYSRLRVLLGTELGGSGAALLAFLGALLEEL
                     DPRTIDARGVPYRAT"
     gene            3650..>4537
                     /locus_tag="PLQ29_05575"
     CDS             3650..>4537
                     /locus_tag="PLQ29_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPG86152.1"
                     /translation="MEARNQAARSAAVLAVLLLIVFGAVATASFIERDFGAVAVSRVR
                     IPVRTNDGLDAYIPGKLYVPALASEANPVPAVLALHGYQNDKDTSAAFALELARRGIA
                     ALAIDQYGHGESPLGLRHRGYDRTKGGPARFKLFMSFSALNGAGVDGLVDSSMGATAA
                     FRWLRDRDYVRGDKVGVTGHSMGTWSAVTVARENPEHAAIVLQCGQPYGPVYGADGSV
                     ELKNYLMLQARYDEFDYFRDYELTTGRLNEAALRYGVFAGQDSPIAWNTTYGRFDDGS
                     ARRMEMIETVHRGVTHSSRA"
BASE COUNT          819 a         1359 c         1470 g          889 t
ORIGIN      
        1 ccgaggtccg cgccaagata gcctactaca aggatctgct cgcccacccc gagaaaatac
       61 ttggcgtgat caaggacgag accaaggtga tctccgacaa attcggcgac gaccgcagaa
      121 cggagatcgt ccacgacgag gtcgaggcta tcaatatcga ggacctgatc accaaggaag
      181 agatggtgat catgctgtcg aacaagggct tcgtgaagcg cgtgccagtc agcgcctatc
      241 ggagccagag ccgcggcggc aaaggctcga attcggcctc gctgctcgac gacgactacg
      301 tgcagcagat cttcgtcgcg aatactcacg actacctgat gtacatctcg agcgccggca
      361 aggcgtactg gcagaaggtt cacgagattc cggaggcctc gcgagcggcc cgcggcggcc
      421 acctcaagag cctcctcgcg atcggggcga acgaggagat tacgaccgtc atcgatctca
      481 agggcttctc cgacgatacc tacctgatgc tcgggacgct gcgcggcgtc gtcaagcgcg
      541 tggcgaccag ccagttcgtc aacgcgaaga ccaggggcat catcgccatt cacctcgacg
      601 agggagaccg gcttgtatcc gcctgcagaa cgcgcggtaa cgacgaggtc gtgctcatta
      661 cccgtctcgg gcaggcgctt cgtatcagcg agcgcgatgt gcgtgtgatg ggccggggta
      721 gccgaggtgt ctgcggcatc aagctggcgg acgccgatga actgaacgcg atgctcaggg
      781 tcaatcccga agaacagatg ctcctgatca gcgagaccgg ttacggaaag cgcaccgact
      841 acgataactt caacgcccac gggagagcga cgcggggaca gaggatctac gatccggacg
      901 agaaatccgg agagatcgtc tgcgccatca ccgtcaagga gaacgacgac gtcgtcgtga
      961 tgaccagcct gggtaagacg ctcaaaacca gggccgccga cgtacggctc tgcggcaaga
     1021 ccgcccgcgg cgtgcatatc gtcaatatcg acagccccga cttcgtgatc ggcatggacc
     1081 gcatcatcaa cgaagaggcc gagatagcgg tctcgaagca ggctcatgac gatgacgcca
     1141 agaaccgcga gtcggaggac gagccagttg atacggtagc gtacgacgat gaacccgagg
     1201 agctaccccc tgacgccgat gacggcgagg tcctggacga ttagaggctc ctgaaaagct
     1261 tctgcctgac acggccgcga ttccacatgg gatcgcggcc tttttaaatg gctagccctg
     1321 gatgtttcac gtgaaacagg cgacgaagct ctgtccccga gacgagcctc tgtccctctc
     1381 gcgatgtttc acgtgaaacg ctagacttgt gctgtgagca tgtggatcgg aatcagtatc
     1441 ggctcgatgc gcgtgaacgg atgggctcga tacgaggcgt gttcgacacg gataggtcga
     1501 tggcgaacgc cgcgatacgg ctcggaatga tcgcacccgg ggctcgtctc tgcgtggatg
     1561 gctataccgt gccgttcccg ctatggaagt atactctcgg tgtgccgtta ttcgttgcga
     1621 cagacatgct attacgagac gttggcacgt cgcatgggcg cttctccggc acggacgggt
     1681 gtacgctcgc gctttaaagc ctggccaacg gccttgagcc cgtcgctatc ggcgagctga
     1741 cgatagtcct cgacgcgctc agtgtgttta gcgctatact cgcggcggcg ggctccctgg
     1801 tcgaggggtt cgacggcgac gcgatagcga gttcggattc ggcgctcggt tctctgagct
     1861 atccgccagg atgggctata ccgactacga ctcgccatag tacggtgcga cgaggaggaa
     1921 tagcggtgac taccgatggg aagcggcgac tcaagggaat tctgctcgtt aacgtcgcga
     1981 cctgcgcctg ggcgacgaat ataatgatcg gccggctcgt gcgagatcag atcggcccgc
     2041 tgacgctgac cgctacgcgc tatgtcgtcg cctctatcgt cttcgcgttt gtcctacgcg
     2101 cgagaccgat ccaggaacgg cgtctcggaa aggattggct cctgctcggg gcgatgtcgc
     2161 tgaccggtgt cgtgttgttc gcccctaccc tatatttcgg cttatcctat acgcaggcga
     2221 tcaatggtac cctgatcaac ggcatcggtc cgctcttgac cgcggcgttc gcggcctggt
     2281 tgctgcgaga gccattttcc ggacggcagc tgttcgggtc ggtcctggcg ctcggcggcg
     2341 tcgtggtcct gttgttcggc tcggcctcgg gtccaagcaa agctctgtcc ctcaaccgcg
     2401 gcgacctgct gatcgtagtg gccgtgacgt tctgggcgct ctattcggta gcgacgcggc
     2461 gcgtggttcg cgaccgttcg ccgatttctg ccacggccct gtcgatgttc atcggcttgc
     2521 ccgtgttgct cgttttcgcg gtcctcgagc atgggcttca gccggtggcc tggacgcctc
     2581 gccttatcgg gctggtcgtg tacgtcgggc ttgtgccggc ggcgctcgga ttcttgtgct
     2641 ggaacgctgg tgtcaagctg cttggcgccg ggggcgccat ggtgttctat aataccttac
     2701 cgctctatgg cgtgctgttc ggtttcctgt tgctcggcga gacccttggg ctcgcgcatc
     2761 tcgccggagg cgcgttgata ctcggcggcg gactcgtcgc ggccatttcg aagcgggcct
     2821 cgcacttggc cgttaataaa taataatgtc attggattcg gctcgacgtc atggctaatc
     2881 gtttcattcg cggaatcgac gacggtgaca tcacgaccga gggcgagctt cgctctgcct
     2941 tcagggcgca ggcgctcgcg acgcatcccg acctgggggg cggaaacgat ggatcggccg
     3001 ccggaaacga tttcatgcgc gtccggacgg agtacgaggc ggctgtacga tatcttgcta
     3061 ggccggctgg agaccagcgg ggagctctgt cgcgcagcgc ggcgagcgcc ggtttcgacc
     3121 gtgggttgtt ctacgccgac ctggcgatcc tgatcaaggc cggctttccc aaacggccgc
     3181 gccacgatca ggagcggcgc aaatactcgc gacttcgcct caacctgcgt tcctctctgt
     3241 ccaggctggc cgccgagacg ctcgccgatc ctctcggggc gttcgacgcg ttcgaggcgg
     3301 cgctattctc gctcagggac agtggaaacg acgaagctac cgttatcgtc gaccgcgttc
     3361 gttccctgct cgaggatatg attgactacg aggcctgtgg cctgacgcca ttgcgagcgg
     3421 ctatagcgat cgaatactcg aggttgcgag tcttgctggg gacagagctg gggggctccg
     3481 gcgcggcgct cctcgccttc cttggcgcgc tactcgagga gctggatccg aggacgattg
     3541 atgcgcgagg ggtgccgtac cgtgctacgt agccgcccgc ctgcgcttgt cttccgcgta
     3601 gagtgtgcga tactgatcgc atgtcgtttc tgcttacaag gacgtcgaca tggaagcacg
     3661 taaccaggcg gcgcggtcag ccgcggttct cgccgtcttg ctcttgatcg tcttcggggc
     3721 cgtcgcgacg gcgtcgttca tcgaacggga cttcggcgcg gtggccgtgt cgcgcgtacg
     3781 catccccgta cggacgaacg acgggctcga cgcctatatt ccgggcaagc tctacgttcc
     3841 cgcgttagcc agtgaggcga atcctgtacc cgccgtgctg gcgctacacg gctatcagaa
     3901 cgataaggat acctccgccg cgttcgcgct cgagctggcc aggcgcggca tcgcggctct
     3961 cgcgatagac cagtatggcc atggcgagag tccgctcggc ctgaggcata ggggctacga
     4021 tcggacgaag ggcggtccgg cgcgtttcaa gctgttcatg agcttctcgg cgctgaacgg
     4081 ggccggcgtc gacgggctcg tcgactcgtc gatgggcgcc accgcggcct tccgctggtt
     4141 acgcgaccgg gactacgtgc gcggtgacaa agtcggggtt accgggcact ctatgggtac
     4201 ctggagcgcg gtcacggtcg ctagggagaa ccccgagcac gcggccatcg ttctccagtg
     4261 cggccagccg tacggcccgg tctacggggc ggacggatcg gtcgagctca agaactacct
     4321 gatgctccag gcccgctacg acgaattcga ctacttcagg gactacgagc tgacgacggg
     4381 gaggctgaac gaagcagcgc tacgctacgg cgtcttcgcc ggtcaggact cgcccattgc
     4441 ctggaatact acctacggcc gcttcgacga tggtagcgcc aggcgcatgg agatgataga
     4501 gaccgtgcac cggggcgtga cccatagctc gcgtgct
//
LOCUS       DAOPHV010000162         4535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_25764_length_4535_cov_4.835045, whole genome shotgun sequence.
ACCESSION   DAOPHV010000162 DAOPHV010000000
VERSION     DAOPHV010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25764_length_4535_cov_4.835045"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..1504)
                     /locus_tag="PLQ29_05580"
     CDS             complement(188..1504)
                     /locus_tag="PLQ29_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPG86153.1"
                     /translation="MATENAKKKGALDWYFKTNLLRRILIGLVLGAIVGVVLGFFPDA
                     VGPYVGAIKFFGDLFIRLLKMIVVPVILFSLITGAASIAPSRLGRVGVKVMIYYLATS
                     AFAVIIGLVFANIFRPGLGFNIVGAAGVAGKAAAAPTISQILLNIVPTNPVESLTKGD
                     VLPIIFFAVVFGLGISFVKDSKNEKIAKGADTLYDVCNAAAETMYKIVGGIMQYAPVG
                     VFVLISIVFAQQGPKAIGPLLIVTLTVYIGLLVHLFGVYGGILAVNKLGFIRFWKGAN
                     EAIITAFVTRSSGGTLPITLRVTEENLGVPRTISSFSLPLGATINMDGTAIYLGVCAM
                     FIGYATGNPLTFNQQLTVVITATLASIGTAGVPGAGAIMLLMVLESIGLKVTEGSAVA
                     AAYAMILGIDALLDMGRTALNVAGDMVGTAIVAKSEKELDLAKWGK"
     gene            complement(1616..2284)
                     /locus_tag="PLQ29_05585"
     CDS             complement(1616..2284)
                     /locus_tag="PLQ29_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPG86154.1"
                     /translation="MSLGAGAIVTIAIVEDQREVREGLTYLLGLDGRVRVIGGFDAAE
                     GLLDWMAGAEKRDEPDIVLMDIHLPGMDGIEATRVLRSARPNMVVLILTVFEEPDTIL
                     AAFRAGARGYLLKNTRPEALLDQVLSALDDGSPISPTVARRLLDELRRDADEGASADY
                     GLTAREREVLRDIVDGLTYKELAEKHHIAGSTAKKHMLHIYQKLNVSSKAEIVRKALS
                     EHLV"
     gene            complement(2281..4071)
                     /locus_tag="PLQ29_05590"
     CDS             complement(2281..4071)
                     /locus_tag="PLQ29_05590"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPG86155.1"
                     /translation="MEKGRWGISHKFIALVSAAVAAFMLSAFLLTRTMLERYALEASD
                     EAAELILDQTDKRLVAFFSELEALARSLASTAVVRSADRAGMRDLFVSSVLARRGYLR
                     AIYLGGSDGRMHEWGVGAEFVDYEPSFPPGYDPRLRPWYLSALERDGFSISAPYRYAS
                     VDDIGITCVLPVTGRDGSPVGVLGLDILLSSLAAVLDGLQIPKDGKAIVLGSNGEIIA
                     SQFHGDRPEGMILKRFAAGDALGSDAGSFVGSVDGMDTQYVYRRVDRLGWIVMVAMPL
                     EPIRESVKALLNLIGVTELALMVALVVALAGISSRLIVSPLGHIVSVINRVEAGQRGL
                     RAQVASSDEFGLLATEFNRLLDAVEENSSSLEGKVRLRTAELGRLQRENTRLKVIEER
                     RRIYRDLHDTIGAKLTNIFFCNGVALDLAKDGPERLKAMLEGVESNCLQAVDSLKRVI
                     LGMGEDDANAGSFSLFVSAGVRQRLAARGMAFDCVIKDKRAIDALPAGLRDELERILD
                     ELVSNALKHSEAGSVRLRLHLRAGALHLAFSDDGVGFDPRARSSGSGLGNIRYRVEGL
                     GGTLSVDTAEGQGSGYRIVVPVDGLPEARS"
     gene            complement(4073..>4535)
                     /locus_tag="PLQ29_05595"
     CDS             complement(4073..>4535)
                     /locus_tag="PLQ29_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPG86156.1"
                     /translation="GLPDTLYAPLGNGTLVLGMYHALKELRSLGLIVSYPKLVAVQAE
                     RCAPVYRASVAGRLSVDPVRNDGTEAEGIAIAAPLRGSQVLAAIRELGGEVAIAPEAG
                     LSPAKKAAAARGFYIETTSAACLAAYFDRRARGVDEGRVALPLCGSGLKSA"
BASE COUNT          787 a         1477 c         1520 g          751 t
ORIGIN      
        1 tggcgtgcaa cgccacgccg gccatcggcc ccactccgcc gcctatgacc acgaccttct
       61 cagcttccat cgtcgcctcc gatcccatag cggcctccga tcccacagta gcctccgaga
      121 aaaagccggg cacccatgat cgggcgcccg gccactatac gcttctacgc gactcggcgg
      181 atggacctta cttgccccac ttggccaagt cgagttcctt ctcgctcttg gcgacgatag
      241 ccgtcccgac catgtcgccg gcgacgttca gggcggtgcg ccccatgtcg agcagggcgt
      301 cgatgccgag tatcatcgcg taggcggcgg cgacggccga gccctcggtg accttgaggc
      361 cgatcgactc gaggaccatg agcagcatga tggcgccggc gccgggcacg ccggccgtgc
      421 cgatcgacgc gagcgtggcg gtgatgacga ccgtgagctg ctggttgaag gtcagcgggt
      481 tgccggtggc gtagcctatg aacatggcgc agacgccgag gtagatcgcg gtgccgtcca
      541 tgttaatggt cgcgccgagc ggcaggctga acgagctgat cgtgcgcggc acgccgaggt
      601 tctcctcggt gacgcgcagg gttatcggca gggtgccgcc ggacgagcgg gtgacgaagg
      661 cggtgatgat cgcctcgttc gcgcccttcc agaagcggat gaagccgagc ttgttgacgg
      721 ccaggatgcc gccgtacacg ccgaacaggt ggacgagcag gccgatgtag acggtgagcg
      781 tgacgatcag gagcgggccg atggccttgg gaccctgctg cgcgaacacg atggagatca
      841 ggacgaacac gccgacgggc gcgtactgca tgatgccgcc gacgatcttg tacatcgtct
      901 cagcggccgc gttgcatacg tcgtagagcg tatcggcgcc tttcgctatc ttctcgttct
      961 tcgagtcctt gacgaacgag atgccgaggc cgaagacgac agcgaagaaa atgatcggca
     1021 gcacgtcgcc cttggtcagc gactcgaccg ggttggtggg gacgatgttg agcaggatct
     1081 gggagatggt aggggccgcg gccgccttgc cggccacgcc ggccgcgccg acgatattga
     1141 aaccgaggcc cggcctgaag atgttggcga acacgaggcc gatgatcacc gcgaaggcgc
     1201 tcgtcgccag gtagtagatc atcaccttga cgccgacgcg cccgaggcgc gacggggcga
     1261 tgctcgcggc gccggtaatc agcgagaaca ggatgaccgg tacgacgatc atcttgagaa
     1321 gcctgatgaa taagtctccg aagaacttga tggccccgac gtaggggccg accgcgtcgg
     1381 ggaagaagcc gagcacgacg ccgactatgg cgccgagcac gagaccgatg aggatgcgcc
     1441 tgaggagatt ggtcttgaag taccaatcga gcgcgccctt cttcttggcg ttttctgtag
     1501 ccatggatgc ctccggatac gtttggtacg cccccggcaa ggaggcgtgg atcgatacgc
     1561 gaagtgtaag ccctgatttc ggatcgcgat atgcaccaaa aggagtattt tacgcctaga
     1621 ccaggtgctc gctcagcgcc ttgcgtacga tctcggcctt ggacgatacg ttcagcttct
     1681 gatagatatg gagcatgtgc ttcttggccg tggagccggc gatgtggtgc ttctcggcca
     1741 gctccttgta ggtcaacccg tcgacgatgt cgcggagcac ctcgcgctcc ctagcggtca
     1801 ggccgtagtc ggccgaagcg ccctcgtcgg cgtcgcggcg caactcgtcg agcagccgcc
     1861 tggccacggt ggggctgatc ggcgagccgt cgtcgagggc gctcaacacc tggtcgagca
     1921 gcgcctcggg gcgggtattc ttgagaaggt agcccctcgc tccggccctg aaggccgcca
     1981 gtatggtatc gggttcctcg aacacggtca ggatcagcac gaccatgttc ggacgggcgg
     2041 accgcaggac gcgggtcgcc tcgatgccgt ccatgccggg cagatggata tccatgagta
     2101 cgatatccgg ctcgtcgcgc ttctcggccc cggccatcca atcgagcagc ccttcggccg
     2161 cgtcgaagcc gccgattacc cgcacccgcc catccagccc gagcaggtag gtcaggcctt
     2221 cacgcacctc gcgctgatcc tcgacgatgg ctatggtcac aatcgcgccg gctccgaggc
     2281 tcatgatcgc gcctccggca ggccgtcgac cggcacgacg atgcggtagc cggagccttg
     2341 gccctcggcc gtatcgacgc tcagcgttcc gcccagtcct tccacccgat accgaatatt
     2401 cccgaggccc gaaccggagg acctggccct cgggtcgaag ccgacgccgt cgtcggagaa
     2461 cgccagatgc agggcgccgg ctctcaggtg cagacgcagc ctgaccgagc ccgcctcgga
     2521 gtgcttgagc gcgttgctga ccagctcgtc caggatgcgc tccagttcgt cgcgcagccc
     2581 ggcgggcagt gcatctatgg cgcgcttgtc cttgatgacg cagtcgaacg ccatgccgcg
     2641 agcggccagg cgctgcctga cgcccgccga tacgaatagg gagaacgagc cggcattcgc
     2701 gtcgtcctcg cccataccca ggatcacgcg cttgaggctg tccaccgcct gtaggcagtt
     2761 cgactcgact ccctccagca tggccttgag ccgctcgggc ccatccttgg ccaggtcgag
     2821 ggccacgccg ttgcagaaga atatattcgt cagcttggcg ccgatggtgt catgaaggtc
     2881 gcggtaaatg cgtcttcgct cctcgatcac cttgagcctc gtgttctccc gctgcagccg
     2941 cccgagctcc gcggtgcgca gccgcacctt gccctccagg ctcgacgagt tctcctcgac
     3001 cgcgtccagg aggcggttga actcggtggc caaaaggccg aactcgtcgg agctcgctac
     3061 ctgagcccgt agcccccgct ggccggcctc gacgcggttg atgaccgata cgatgtggcc
     3121 gagcggggag acgatgagcc ggctcgatat gccggccagc gcgacgacga gggcgaccat
     3181 gagcgctagc tcggttacgc ctatcaggtt caggagcgcc ttgaccgatt cgcgtatcgg
     3241 ctcgagcggc atcgcgacca tgacgatcca gccgagccta tcgacacgtc ggtagacgta
     3301 ctgggtgtcc atgccgtcca ccgatccgac gaacgacccg gcgtccgagc cgagcgcgtc
     3361 gcccgccgcg aagcgcttga gtatcatgcc ctccggccta tcgccgtgga actggctggc
     3421 tatgatctca ccgttggagc cgagcacgat ggccttgccg tccttgggta tctggagtcc
     3481 gtcgagcacg gccgccaggc tcgacagcag gatgtccagg ccgagcacgc cgacggggga
     3541 gccatcccga ccggtcacgg gcaggacgca ggtgatgccg atgtcgtcga cgctggcgta
     3601 gcggtacggc gcggatatgg agaaaccgtc acgctccagg gcgctcaggt accagggccg
     3661 cagcctggga tcgtagccgg gcgggaagct gggctcatag tcgacgaatt ccgccccgac
     3721 gccccattcg tgcatacggc cgtcggagcc gccgaggtag atggcgcgta gatagccgcg
     3781 ccgggccagc acggacgaca cgaacaggtc gcgcatgccc gcccggtcgg cgctcctgac
     3841 cacggcggtc gacgcgaggc tcctggccaa ggcttcgagc tcggagaaga acgcgacgag
     3901 ccgcttgtcg gtctggtcga gtatcagctc cgcggcctcg tcggaggcct cgagggcgta
     3961 gcgctcgagc atggtgcgcg tcaagaggaa ggccgacagc atgaatgccg cgaccgccgc
     4021 cgacacgagc gcgatgaact tgtggctgat cccccagcga cccttttcca tgctaggcgc
     4081 tcttgagtcc cgagccgcac agcggcagag cgacgcggcc ctcgtcgacg ccgcgagccc
     4141 tgcgatcgaa gtaggcggcg aggcaggcgg cgctcgtcgt ctcgatatag aagccgcgcg
     4201 ccgcggcggc ctttttggcc gggctcagcc cggcctccgg tgcgatggcg acctcgccgc
     4261 ctagctcgcg gatcgctgcc aggacctgac tgccgcgaag cggggccgcg atggcgatac
     4321 cctcggcctc ggtaccgtcg ttccggaccg ggtccacgga gagccggccg gcgacggagg
     4381 cgcggtagac cggagcgcag cgctcggcct gcacggcgac gagcttagga taggagacga
     4441 tgagtccgag cgaacgtagc tccttgagcg cgtggtacat cccgagcacc agagtcccgt
     4501 tgccgagcgg cgcgtagagt gtatcgggta ggccg
//
LOCUS       DAOPHV010000163         4531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_25804_length_4531_cov_4.239500, whole genome shotgun sequence.
ACCESSION   DAOPHV010000163 DAOPHV010000000
VERSION     DAOPHV010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25804_length_4531_cov_4.239500"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="PLQ29_05600"
     CDS             <1..1262
                     /locus_tag="PLQ29_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPG86157.1"
                     /translation="EAMAAVNGSELKAVGIDWNLAPVADVNVNGLNPVIGVRSFGEDP
                     EAVAELTAAYARGLSKAGVASCAKHFPGHGDTSVDSHLDLPRVEADADRLERVELAPF
                     RRLIAEGVSAIMSAHVLFPAVEPEPIPATLSRKALTGLLRQRLGFRGLIVTDCLEMRA
                     VGGRYDDLAARAILAGADVLCVSHTAELQEEAFDSLLAAVRSGRIPESRVDESAARVL
                     ALKAVLASQASARDPLGRPESLALATRLADASISLVSGALPDLGGGCLYLDHRPALES
                     RVEDGHEATPSMAASIGAAMADRAFGAIELPVDPDARAINAAVAAAAASSRPVVMGSY
                     SLARHPAQAALAEALAAACDEAGVPLCFVATREPYDAAALSAIGGKERAVLCAYEYTE
                     LSTRAVARVLSGSAVPAGTCPVSCRFL"
     gene            1315..2487
                     /gene="nagA"
                     /locus_tag="PLQ29_05605"
     CDS             1315..2487
                     /gene="nagA"
                     /locus_tag="PLQ29_05605"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HPG86158.1"
                     /translation="MATVCIHNATLLAGYAKMQSCAVLVEDGRIADVFSEKRLAQKRF
                     PAGAAVYDAQGAYIAPGFIDTHIHGFAGYGTEDMSVDAVMAMSDRLGAYGVTSFCPTI
                     YPMELGDMERSIEACVGAMGRETGAKVMGVHLEGPFVSMARLGVQRPEFVRPVDLEVM
                     ERLFKAGRGHITNMTVAPELKGMRELALYCLSRGIVLQAGHTDATYENMMEGMQAGIL
                     HSTHFFNAMSRLHHRDPGAVGAILINPELSCEIIADGRHVHPDLIRLLLRDKPQSKVV
                     LVTDALKPTEQERCPLYANGEEVVMEDGVFHRKSDGVIAGSCLTMIAGVRNLVDWGVA
                     VEAAVEMAASNPARIVGLGKRGLLVPGYEADLVVFGKDFSVLASMVGGRFIKNELA"
     gene            2505..3305
                     /gene="nagB"
                     /locus_tag="PLQ29_05610"
     CDS             2505..3305
                     /gene="nagB"
                     /locus_tag="PLQ29_05610"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002665046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HPG86159.1"
                     /translation="MRLIIRKNYEAVSEWAANHIAGRVRSTPTGSTCVLGLPTGSTPL
                     GVYDRLASLVARGELSFGNVVTFNMDEYVGLPEDHPQSYHSFMRERLFSRVDIDPANA
                     HLLDGNAPDLEAECARYEAAIAAAGGIDLFVGGVGEDGHIAFNEPGSSLSSRTRVKTL
                     TRDTRAVNARFFGGDPAAVPATALTVGVGTIMDAREVMILVSGPAKARALRHAVEEGV
                     NHLWTVSCLQLHPRLVIVCDERATDELKVGTVRYFNEIEAGNFNNSIA"
     gene            3439..>4531
                     /gene="glgP"
                     /locus_tag="PLQ29_05615"
     CDS             3439..>4531
                     /gene="glgP"
                     /locus_tag="PLQ29_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPG86160.1"
                     /translation="MKILTHTIKPKIPEALAPLEEMAHNLWISWNFDAIMLFIRLDFE
                     VWGHSRQNPAKMLGMVSQARFDEAAADDSFLAALREVYAKFQAYRDGQSWYKGSYEDS
                     VAYFSMEYGLDSSLPIYSGGLGILSGDHMKTSSDLGLPLVGVGLLYRQGYFRQYLNAD
                     GFQQEEYPENDWYNMPVYRREDASGQPVKITVDMAGEQVVAQVWEVKVGRSSLFLLNT
                     NIEENTPDNRLITATLYGGDKETRIRQEILLGIGGIRALKAIGVNPVVAHMNEGHAAF
                     LGLERVRTAMEGHGLSFAQAAQALWSTNIFTTHTPVPAGNERFGVDLMEKYFKGWASM
                     LGLDWKAFLALGSERPNDDAEPFCMTVLAL"
BASE COUNT          763 a         1569 c         1477 g          722 t
ORIGIN      
        1 tcgaggcgat ggccgcggtc aacggctcgg agctcaaggc cgtcggcatc gactggaacc
       61 tggcgccggt agcggacgtc aatgtgaacg gcctcaaccc ggtcataggc gtgcgcagct
      121 tcggcgagga tcccgaggcg gtggccgagc tgacggccgc ctacgctcgc gggctgtcca
      181 aggccggcgt cgcctcctgc gccaagcatt tcccgggcca cggagacacg agcgtcgact
      241 cgcacctgga cctgccccgc gtcgaggccg acgccgatcg gctcgagcgc gtggaactgg
      301 ccccattccg gcggctgata gccgagggcg tctcggccat catgtcggcc catgtcctgt
      361 tccccgccgt cgaaccggag cccattccgg ccaccctgtc gcgcaaggcg ctgaccggcc
      421 tcctacgcca acggcttggc tttcgcggac tcatcgtcac cgactgcctc gagatgaggg
      481 ccgtcggcgg ccgctatgac gacctggccg cacgcgcgat cctggccggg gccgacgtgc
      541 tctgcgtcag ccatacggcc gagctgcagg aggaggcctt cgactcgttg ctcgccgccg
      601 tgcgctccgg gcgcatcccc gagtcgcgcg tcgacgagtc ggcggccagg gtactcgccc
      661 tgaaggcggt tctcgcgtca caggcgtcgg cccgagaccc gctcgggcgg ccggaatcgt
      721 tggcgctggc taccaggctg gccgatgcgt cgatcagcct cgtctccgga gccctgccgg
      781 acctcggcgg cggctgcctc tacctcgacc ataggccggc tctggaaagc cgcgtcgagg
      841 acgggcacga ggccaccccg tcgatggcgg cctcgatagg cgcggcgatg gccgatagag
      901 ccttcggggc gatcgagctg ccggtagacc cggacgcccg agccatcaac gccgcggtag
      961 ccgcggcggc ggccagctcg aggccggtcg tcatgggtag ctactcgctg gccaggcacc
     1021 cggcccaggc agccctggcc gaggccctcg ccgccgcctg cgacgaggcg ggcgtcccgc
     1081 tatgcttcgt cgcgacgcgc gagccgtacg acgccgccgc cctgtcggcg atcgggggca
     1141 aggagcgggc ggtgctctgc gcctacgagt acaccgagct atcgacccgg gcggtcgcgc
     1201 gcgttctgag cggctcggcc gtccccgcgg gcacgtgtcc ggtatcgtgc cgcttccttt
     1261 aatggacggg attggcgata ttcgatagag ctacggatac cgaggagacc gacgatggct
     1321 accgtatgca tccataacgc tacactcctg gccgggtacg ccaagatgca atcctgcgcc
     1381 gtgctcgtgg aggacggcag gatagctgac gtgttctcgg agaagcggct cgcccagaaa
     1441 cgcttcccgg ccggcgcggc cgtttacgac gcgcagggcg cctatatcgc gccgggcttc
     1501 atcgacaccc atatccatgg cttcgccggc tacggtaccg aggatatgtc ggtcgacgcc
     1561 gtgatggcca tgtccgaccg gctcggcgcc tacggcgtca cctcgttctg cccgaccatc
     1621 tatccgatgg agctgggcga tatggagcgc tccatcgagg cctgcgtcgg ggccatgggg
     1681 cgggagaccg gcgccaaggt gatgggcgtg cacctcgagg ggcccttcgt ctccatggcc
     1741 cgcctcggcg tgcaacggcc ggaattcgtc cgcccggtcg acctcgaggt gatggaacgg
     1801 ctcttcaaag ccggacgggg ccatatcacc aatatgaccg tggcgccgga gctcaagggc
     1861 atgcgggagc tggccctgta ctgcctgtcc aggggtatcg tgctccaggc tggccacacc
     1921 gacgctacct acgagaacat gatggagggc atgcaggcgg gcatactcca ctcgacccac
     1981 ttcttcaacg cgatgagccg cttgcaccac cgcgaccccg gtgccgtcgg cgccatcctg
     2041 atcaacccgg agctatcctg cgagatcata gcggacggcc gccacgtgca ccccgacctg
     2101 atacggctac tcctgcgcga caagccgcag agcaaggtcg tactcgtcac cgacgccctc
     2161 aagccgaccg agcaggaacg ttgcccgctc tacgccaacg gcgaggaggt cgtcatggag
     2221 gacggggtgt tccaccgcaa gagcgacggc gtgatagcag gctcgtgctt gacgatgata
     2281 gccggcgtgc gtaacctcgt cgactggggc gtcgccgtcg aggccgccgt cgagatggcc
     2341 gcgagcaacc cggcccgcat cgtcggactg ggtaagcgag gcctcctggt acccggttac
     2401 gaagccgacc tcgtcgtgtt cggcaaggac ttttcagtgc tggcatcgat ggtaggcggc
     2461 cgcttcatca agaacgagct ggcctaaggc gaaaggaacg cgtcgtgcgg ctgatcatcc
     2521 gtaagaacta cgaggccgtc tccgaatggg cggcgaacca tatcgccggg cgcgtccgct
     2581 cgacgccgac cggctcgact tgcgtcctcg gactaccgac cggctccacg cccctcggcg
     2641 tctatgaccg cttggcgtcc ctcgtcgccc ggggcgagct gtcgttcggg aacgtcgtca
     2701 cctttaacat ggacgagtac gtcggcctgc ccgaggacca cccgcagagc taccacagct
     2761 tcatgcgcga gcgcctgttc tcccgcgtcg acatcgaccc ggcgaacgcc catcttctcg
     2821 atggcaacgc gcccgacctc gaggccgagt gcgcccgcta cgaggccgct atcgcggcgg
     2881 ccggcggcat cgacctcttc gtcggcggcg tcggggaaga cggccacatc gcgttcaacg
     2941 agccaggctc gtccctttca tcgcggaccc gcgtcaagac gctcacgcgg gacacccgcg
     3001 ccgtcaacgc ccgcttcttc ggcggcgacc ccgccgcggt gccggccacc gccctgaccg
     3061 tcggcgtcgg caccatcatg gacgcccggg aggtgatgat actcgtttcc gggcccgcca
     3121 aggcccgcgc cctgcgccac gcggtcgagg aaggcgtcaa ccacctctgg accgtctcct
     3181 gcctccagct gcacccccgg ctcgtgatcg tctgcgacga acgcgccacc gacgagctca
     3241 aggtcggcac cgtgcggtac ttcaacgaga tagaggcggg caatttcaac aattccatag
     3301 cctgacgcga cgcgaaaggc tcacgacgca ccgcgagcgt agctacgact ggcgcccgac
     3361 ccccgtcgtc gattatgatt agcgcacgcg ctccgtcatg cacgagcgcg atcctaaccg
     3421 agccgacggc ggtgaaccat gaagatcctg acccacacca tcaagccgaa gatccccgag
     3481 gccctcgctc cgctcgagga gatggcccac aacctctgga tatcgtggaa tttcgacgcg
     3541 atcatgctgt tcatcaggct ggacttcgaa gtctgggggc attcacggca gaacccggcc
     3601 aagatgctcg gcatggtgtc gcaggcgcgc ttcgacgagg cggccgcgga tgactccttc
     3661 ctcgcggcgc tgcgggaggt gtacgcaaag ttccaggcgt acagggacgg ccagagctgg
     3721 tacaagggct cgtacgagga ctccgtagcc tatttctcga tggaatacgg cctggactcg
     3781 tccctgccta tctattccgg cggcctgggc atcttgtcag gcgaccacat gaagaccagc
     3841 tcggaccttg gcctgcccct cgtcggcgtc ggcctcctat ataggcaggg ctacttcagg
     3901 cagtacttga atgccgacgg cttccagcag gaggaatacc cggagaacga ttggtacaac
     3961 atgcccgtgt accgccgcga ggacgcttcc ggccagccgg tcaagataac cgtcgacatg
     4021 gccggggagc aggtcgtcgc ccaggtatgg gaggtcaagg tcggccgatc ctcgctgttc
     4081 ctcctgaaca ccaacatcga ggagaacacg ccggacaacc gcctgatcac ggccaccctg
     4141 tacggcggcg acaaggagac gcgcatacgc caggagatac tcctcggcat cggcggcatc
     4201 cgggcgctca aggccatcgg cgtcaatccg gtcgtcgcgc acatgaacga gggccacgcg
     4261 gccttcctcg gcctcgagcg cgtgcggacg gcgatggagg gccacggtct gagcttcgcg
     4321 caggcggccc aggccctctg gtcgaccaac atcttcacga cgcacacgcc ggttccggcc
     4381 ggcaacgagc gcttcggcgt cgacctgatg gagaagtact tcaagggctg ggcctccatg
     4441 ctcgggctcg actggaaggc tttcctggcg ctcggcagcg agcggcccaa cgacgacgct
     4501 gagccgttct gcatgacggt gctggcgctc a
//
LOCUS       DAOPHV010000164         4515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_25914_length_4515_cov_5.115919, whole genome shotgun sequence.
ACCESSION   DAOPHV010000164 DAOPHV010000000
VERSION     DAOPHV010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25914_length_4515_cov_5.115919"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="PLQ29_05620"
     CDS             complement(<1..1070)
                     /locus_tag="PLQ29_05620"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HPG86161.1"
                     /translation="MNRPATRLAPACAVLALCAATAAGQATVVFRTAPPNAALLDASA
                     PGLDTALRLRAADNGGRSAHLTAGTHEITLIAEGYRPARVAVEVAEGPGAPAVVVEAK
                     LERAASVLRLARVIATGRRPKSVEFTPDGRYVVVAPLSGDAVEVIDPRTGTLVANLAP
                     PERWARSQGFVETAFPGRGEVWVSQMYTDLLHVFSLADYSYLRSVSSGGSFPKVLLAH
                     PDGRVFASNWLSETVSALSPDGMTRLGSMRAGSTPRGLALSPSGGTLYVANFGNGSIE
                     AFDARSYARLPAPFGGAAVPLAGSAKRHLVVDPVRNRLYASDMGRGSVFAYDLAADRQ
                     LAEIPIAPNVKTNTIALSPDGR"
     gene            complement(1067..2101)
                     /locus_tag="PLQ29_05625"
     CDS             complement(1067..2101)
                     /locus_tag="PLQ29_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG86162.1"
                     /translation="MRPAVVAAWLAALLALLASSCATGQSRRQAAGWAELGTAWAELG
                     EWDKAGDAWSRAMALDPGQGVAGYNLSRALAEAGKYDESIAMSDRYLESDPDNAAVLA
                     VKAYALHRQGRDDEAIAVYERVASLNGGDRASSFNLAVLLEATGRGDEAMARYDAMLA
                     LDPDDAAASYRKGLLLAGGGDAEAAIPLLERYLAAEPESAEGRRVLAAALERAGLYLR
                     ALEAYAAMAAKDETDAAAWFALARLRLTKADDATGGLSALDSAIEQGFKDAEAARALL
                     RTDGLLAADLVEKRLGRVGLLDGERADGASADGASADGASADGASADGATAGASQGAP
                     KDEGAGDSGS"
     gene            complement(2098..3162)
                     /locus_tag="PLQ29_05630"
     CDS             complement(2098..3162)
                     /locus_tag="PLQ29_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HPG86163.1"
                     /translation="MNKVVLKAQDLDGYLTDGDRAMIERMDGLYRKAMLSFSILSAAR
                     GDGERKKEESNLIALYNEMGGLMQEICKAEPRIQVYSFETAPEHHGEASRLIAKLRDI
                     DTQRQEFVYYTQRAYELLFGLAFGGAKSENKNYLIPRTPVTMPVQNYAVHKIPNIDDR
                     IDNTVMCVMLRGALLPSMIVSKEIQEFSSKGYVTPFALFKIKRDDTKNESTMEYVLDL
                     DRSYFRPEELDGKDLVFADPMNATGGSLVTVVKYILDLGVKPRSVKFLTIISALKGAL
                     RAVRAVDNMTVYTLWMDPALNEMAYIMPGLGDAGDRINGRDLDDTPRDMIQLIADYGA
                     GIASLYRAQIRTIEETVLRR"
     gene            complement(3262..3846)
                     /gene="pduL"
                     /locus_tag="PLQ29_05635"
     CDS             complement(3262..3846)
                     /gene="pduL"
                     /locus_tag="PLQ29_05635"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: protein motif:HMM:NF011652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HPG86164.1"
                     /translation="MPETKVLINLSNHHIHLCAADVEALFGKGHTLTKTKDLMQPGQF
                     ACEELVTIKGPKGSIAKVRVLGPERKETQCEILASEMFKLGVPDCQTRESGKLDGSSP
                     FEIEGPAGSVKKDKGLIVAKRHIHFDPASGERFGVKDSEIVDLKVPGERGAVFLNVVC
                     RVNPAYALECHLDFDEGNAVGIGNGAIGEILRRS"
     gene            complement(4098..>4515)
                     /locus_tag="PLQ29_05640"
     CDS             complement(4098..>4515)
                     /locus_tag="PLQ29_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPG86165.1"
                     /translation="ATIFITNGFIRLNRSLSLDGQGDVDQFTTKAIIAYVARKNGLTQ
                     ATARAVVDDYLSMIETGMLLGERVPVGKLGNASLRFQAARKARIVKNLKTGDDLLVPA
                     KPASMAPKFSFSQSVKDKSLSVDPTILSQGDDEEDD"
BASE COUNT          645 a         1556 c         1551 g          763 t
ORIGIN      
        1 cagcggccgt cgggcgacag cgctatggtg ttcgtcttca cgttcggggc tatcggtatc
       61 tcggccaatt ggcggtcggc cgccaggtcg tacgcgaaga cgctaccccg ccccatgtcc
      121 gaggcgtaca gccggttcct gaccgggtcg acgacgaggt ggcgcttggc cgagccggcc
      181 aggggtacgg ccgccccgcc gaacggcgcg ggtagccgcg cgtacgagcg cgcgtcgaag
      241 gcctcgatgg agccgttccc gaaattcgcc acgtacagcg tgccgccgga cggcgacagg
      301 gccaggccgc gcggcgtgga ccctgcgcgc atcgagccga gccgggtcat gccgtcgggc
      361 gacagcgcgc tgacggtctc ggacagccaa ttcgacgcga agacccgccc gtcggggtgg
      421 gcgagaagca ccttcgggaa ggatccgccc gaggataccg agcggaggta cgagtagtcg
      481 gccaacgaga atacgtgcag gaggtcggtg tacatctggc tgacccagac ctcgccacgg
      541 ccagggaagg ccgtttcgac gaagccctgg ctcctcgccc agcgctccgg aggcgccagg
      601 ttggcgacga gagtcccggt gcgcggatcg attacctcga cagcgtcacc ggacaaaggg
      661 gcgacgacga cgtaccggcc gtccggggtg aactcgacgc tctttggccg ccgccccgtc
      721 gctatcacgc gagccagccg cagcaccgag gcggcgcgct cgagcttggc ctcgacgacg
      781 acggccggcg cgcccggccc ctcggcgacc tcgacggcga cgcgggccgg ccggtagccc
      841 tcggctatca gcgtgatctc gtgcgtcccg gccgttaggt gcgcggaccg gccgccgtta
      901 tccgcggccc gcagccgtag cgccgtgtcc agccccggcg ccgacgcgtc gagcagggcc
      961 gcgttaggcg gggccgtcct gaacacgacc gtcgcctggc cggcggcggt cgccgcgcag
     1021 agcgccagaa cggcgcaggc cggtgcgagc ctcgtggccg ggcggttcaa gagccgctgt
     1081 ccccggcgcc ctcatccttg ggcgcgccct gcgacgcgcc ggccgtcgcg ccatcggccg
     1141 acgcgccatc ggccgacgcg ccgtcagccg acgcgccgtc agccgacgcg ccgtcagccc
     1201 gctccccgtc gaggagcccg acgcgcccga gccgcttctc gacgagatcg gccgcgagta
     1261 gcccgtccgt acggagcagg gcgcgggcgg cctcggcgtc cttgaagccc tgctcgatag
     1321 ccgagtcgag cgccgacagc ccgcccgtgg cgtcgtcggc cttggtcagc ctgagcctgg
     1381 ccagcgcgaa ccaggccgcc gcgtcggttt catccttggc cgccatcgcg gcgtaggcct
     1441 cgagggcacg caggtacaac ccggcccgct cgagtgcggc cgccaggacc cgtcgcccct
     1501 cggccgactc cggctcggcg gccaggtaac gctccaggag cgggatggcc gcctcggcgt
     1561 cgccgccgcc agcgaggagg agccccttgc ggtacgaggc ggccgcgtca tccgggtcta
     1621 gggcgagcat cgcgtcgtag cgggccatcg cctcgtcccc gcgaccggtg gcctcgagca
     1681 acacggctag gttgaacgag ctcgcgcggt cgccgccgtt cagcgaagcg acgcgctcgt
     1741 agacggcgat cgcctcgtcg tcacggcctt gccggtgcag cgcgtaggcc ttgaccgcga
     1801 gcacggctgc gttgtcggga tccgattcca ggtagcggtc gctcatcgcg atcgactcgt
     1861 cgtacttgcc cgcctcggcg agcgcccgtg acaggttgta gccggccacg ccctggcccg
     1921 gatcgagggc catggcgcgc gaccaggcgt cgccggcctt gtcccactcg cccagctcgg
     1981 cccaagcggt gccgagctcc gcccagcccg cggcctgccg cctggattgg cccgtggcgc
     2041 acgacgaggc gagaagcgcc agcaacgcgg ccagccaggc ggcgacgacg gccggcctca
     2101 tctcctgagc accgtctcct cgatcgtgcg tatctgcgcc cgatagaggc tggcgatgcc
     2161 ggcgccgtag tcggctatca gctggatcat gtctcgcggc gtgtcgtcga ggtcgcgtcc
     2221 gttgatgcgg tcgccggcgt cgccgaggcc cggcatgatg taagccatct cgttgagggc
     2281 cggatccatc cacaaggtat agacggtcat gttgtcgacc gcgcggacgg ccctgagggc
     2341 gcccttgagc gccgagatga tggtcaggaa cttgaccgac cgcggcttga cgccgaggtc
     2401 gaggatgtac ttgacgacgg tgacgaggct gccgccggtg gcgttcatcg ggtcggcgaa
     2461 gacgaggtcc ttgccgtcca gctcctccgg cctgaagtac gatcggtcca ggtcgagcac
     2521 gtactccatc gtcgactcgt tcttcgtgtc gtcgcgcttt atcttgaaca gcgcgaacgg
     2581 cgtgacgtag cccttggacg agaattcctg tatctccttg gacacgatca tcgacggcag
     2641 gagggcgccg cgcagcatca cgcacatgac cgtgttgtcg atgcggtcgt cgatgttcgg
     2701 gatcttatgc acggcgtagt tctgcaccgg catggtcacc ggcgtacgcg ggatcaggta
     2761 gttcttgttc tcgctcttgg cgccgccgaa ggccagaccg aacagcagct cgtaggcgcg
     2821 ctgcgtgtag tacacgaact cctggcgctg cgtgtctatg tcgcgcagct tggcgatcag
     2881 cctcgaggcc tcgccgtggt gctcgggcgc cgtctcgaag gaatagacct ggatcctggg
     2941 ctcggccttg cagatctcct gcatcaggcc gcccatctcg ttgtagagcg cgatgaggtt
     3001 agactcctcc ttcttgcgct cgccgtcccc gcgcgccgcg gagagtatgc tgaaggacag
     3061 catggccttg cggtagaggc cgtccatacg ctctatcatc gccctgtcgc cgtcggtcag
     3121 gtagccgtcg agatcctggg ccttgagcac aaccttgttc atccgttcct ccggggccgg
     3181 ccgcccggcg ctctcggcgc cggcggcgat cattgcggcg gccgccgcga aggtggcccc
     3241 gcgactacga accgaacgcc gtcaggaccg gcgcagtatc tcgcctatgg cgccgtttcc
     3301 gataccgacg gcgttgccct cgtcgaagtc gaggtggcac tcgagggcgt aggccgggtt
     3361 gacgcggcag acgacgttga ggaacaccgc gccgcgctcg cccggcacct tgaggtcgac
     3421 gatctcggaa tccttgacgc cgaagcgctc gcctgaggcc gggtcgaagt ggatatggcg
     3481 cttggcgacg atcaggccct tgtccttctt gaccgagccg gccggcccct cgatctcgaa
     3541 ggggctggaa ccatcgagct tgccggactc gcgggtctgg caatcgggca cgccgagttt
     3601 gaacatctcg ctggccagga tctcgcactg cgtctccttg cgctcggggc cgagcacgcg
     3661 gaccttggcg atcgaaccct tgggcccctt gatcgtcacg agctcctcgc aggcgaactg
     3721 gccgggctgc atcaggtctt tggtcttggt cagcgtgtgg cccttgccga atagggcctc
     3781 gacgtcggcg gcgcagagat ggatgtgatg gttggacagg ttgatgagaa ccttggtctc
     3841 gggcatgatg gctccttgat ggccgctacc ggcggccgtg atcgtaggcg ggacgacggc
     3901 cgaagagccg cgtcgtcgaa gtatagcaaa tgccgggccg cttgtcactg cggcgcgcga
     3961 ggccgccggc gccccgcctc gtcgtcatcc tcgtcgtcat cctcgtcgtc atcctcgtcg
     4021 tcatcctcgt cgtccgccta gtcgtcatcc tcgtcgtccg cctagtcgtc atcctcgtcg
     4081 tcatcctcgt cgtccgccta gtcgtcttcc tcgtcgtcgc cctgcgacag aatggtcggg
     4141 tcgaccgaca ggctcttgtc cttgaccgac tgcgagaacg agaacttcgg cgccatgctc
     4201 gccggcttgg ccggtacgag caggtcatcg ccggtcttga ggttcttgac gatgcgggcc
     4261 ttgcgcgcgg cctggaagcg caggctggcg ttgcccagct tgccgacggg cacccgctcg
     4321 ccgagcagca tgccggtctc tatcatcgac aggtagtcgt ccacgaccgc ccgcgccgtc
     4381 gcctgggtca ggccgttctt cctggccaca taggcgatga tggccttggt ggtgaactgg
     4441 tcgacgtcgc cctggccgtc cagggagagg ctgcggttca gcctgatgaa gccgttggtg
     4501 atgaagatcg tcgcc
//
LOCUS       DAOPHV010000165         4504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_26013_length_4504_cov_3.315801, whole genome shotgun sequence.
ACCESSION   DAOPHV010000165 DAOPHV010000000
VERSION     DAOPHV010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26013_length_4504_cov_3.315801"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="PLQ29_05645"
     CDS             complement(<1..1030)
                     /locus_tag="PLQ29_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG86166.1"
                     /translation="MEYDAVVVGAGMAGLTAAAYLCKAGKSVALLEKRGKTGGLVDSF
                     ERNGFTFDGGIRAVENSGIVFPMLRQLGVELEFLQSPVSIGFGDDVLRLSPEAGLDEY
                     RALLRRRFPGIVSEVDAIVDEVARVMDYMAVLYGIDNPLFLDLKDDPGYVLKVILPWM
                     LKYMTTMPKVARLRMPVDERLAALTGSRELADMIGQHFFKKTPAYFALSYFSLYLDYR
                     YPRGGTGRLPEALESYIRDRGGSVMTGVEVAGLDAARRVVTDASGSEYGYRRLVWAAD
                     SRLLYRLALASPLADRGVARRVEAKAAELASARGGDSIFTLYVAADLPPVFFSSVSSA
                     HFFYTPRGE"
     gene            1197..3317
                     /locus_tag="PLQ29_05650"
     CDS             1197..3317
                     /locus_tag="PLQ29_05650"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG86167.1"
                     /translation="MPNRTNSLSLPPVIAVDEGTCVNCHMCIAVCPVKYCIDGSGDKV
                     TINPDLCIGCGSCVKVCTQKARVIIDDAARFVEALGRGEKLVAVVAPAIAASYPGEHR
                     RFLGWLKGLGVSACFDVSFGAELTVRSYLRHIEAARPELVIAQPCPAIVNYIEIYRPE
                     LLGYLAPADSPMLHTIKMIREYYPAYRDHKILVVSPCAAKKREFEETGAGDYNVTLRS
                     LDDMLKQRKVDLSRQPEAEFENSPAERAVLFSTPGGLLRTVERDLPDVRAVARKIEGP
                     ELVYPYLDTLPEALGKGVNPLLVDCLNCEYGCNAGPGTLNQDLAPDVIEWAVERRAKE
                     GRVAYAGTRGDDRVARRRVEKALSRYWKPGLYARTYVDRSASYALRIPTNAELEALYA
                     DMLKKGAEDLLNCAACGYKSCRGMAIAIHNGLNKKENCHLFKQRVIEREQRIVDEASV
                     RLHEEIQRMLEDVRAISGELSSLHSRSEAQFEAIQASSTAVREMVDTLSSDSGIASGK
                     RTQIMALAGAAEDGESGMRSTMETIRQTAAEVSGIGSMMDVIQDVADTTNLLAMNAAI
                     QAARAGTAGKGFAVVAGEIRRLAEATKSQADGIGASLGSIIEQIASSDDLILRTEASM
                     QAMSRDVSVMAEEMSGLIDSFSGLSSGGATVMAGIDELKTATGEVRSIYANISVELER
                     LFSRIGAIASISERTQATISAMSR"
     gene            3388..>4504
                     /locus_tag="PLQ29_05655"
     CDS             3388..>4504
                     /locus_tag="PLQ29_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPG86168.1"
                     /translation="MKRDLSSGSVLGNLLAMSVPTMFGMLGQTLYDVIDMIWIGRISG
                     EAVAGVALFSSIFWLVEILNEIVGVSSVAMITQSYGSGDLARTGRIIEQTIVFKALMA
                     IIAALALNLVLEPLIRFFSDDPEVLRSALEYGRVRIYFLPVFFATYSCFTALRCTGDP
                     ASQMWIMLGASVLNTVLDPILMFETVPGLGWRGCGLGVFGAGLATVISICLAFAVGFG
                     LLARGRGGVRLTPAGLFRLDPAIDRKLLTIGLPSGGEMLARQAAGLVTTKFVAAYGTA
                     AIAALGIGNRLGGLVFMPLFGLMSGGGTIVGQNLGAENVERAEKTAKAASLFGGLLIT
                     ALMGLAIAFPRPVMAVFVDSAETIEAGVSMIRLMGSSF"
BASE COUNT          791 a         1483 c         1470 g          760 t
ORIGIN      
        1 cctcgccgcg cggcgtgtag aagaagtgag cgctcgacac ggacgagaag aacacgggcg
       61 gcaggtcggc ggcgacgtac agcgtgaaga tcgagtcccc gccgcgcgcc gacgcgagct
      121 cggccgcctt ggcctcgacg cgcctggcga cgccccggtc ggctagcgga gaggctagcg
      181 ccagccggta caggagccgc gagtcggctg cccagacgag cctgcggtag ccgtactcgc
      241 tcccggacgc gtcggtgacg acccgacggg ccgcgtcgag gccggcgacc tcgacgccgg
      301 tcatcaccga gccgcctcgg tcgcgtatat acgactcgag ggcctcgggt aaccggccgg
      361 tgccgcccct ggggtagcga tagtcgaggt acaggctgaa gtacgacagg gcgaagtagg
      421 ccggcgtctt cttgaagaag tgctggccaa tcatatcggc caactcgcgg ctgccggtca
      481 gggccgccaa ccgctcgtcc accggcatgc gcagcctggc caccttgggc atggtcgtca
      541 tatacttgag catccaaggc aggatgacct tgagtacgta gcccggatcg tccttcagat
      601 cgaggaacaa ggggttgtcg atgccgtaga ggacggccat gtaatccatc accctggcca
      661 cctcgtcgac tatcgcgtcg acctcggaga caatcccggg gaaacggcgg cgcagcaagg
      721 cccggtattc gtcgagaccg gcctcgggcg atagcctgag tacgtcgtcg ccgaagccta
      781 tcgacacggg gctctgcagg aactccagct cgacgccgag ctgcctgagc atcgggaaca
      841 cgatgcccga gttctcgacg gcgcgtatgc cgccgtcgaa ggtgaacccg ttccgttcga
      901 acgaatcgac gaggccgccg gtcttgcctc gcttctcgag caaggctacc gatttgcccg
      961 ctttacagag ataggcggcg gcggtcagcc ccgccatgcc tgcgccgacc acgacggcat
     1021 cgtactccat ggttcttcct gacgtttcag tatagaggaa agcctatcaa actattacct
     1081 cgggtcggcg gcttgtcaat ctcgatttga tcgcccggcc atgttactat acgtgatcgc
     1141 ttgcacatga actcgctcgt gtatagaatt cgaccccaat acggagacta gccaccatgc
     1201 cgaaccgaac gaactccttg tcgctaccgc cggtcatagc ggttgacgag ggtacctgcg
     1261 tcaattgcca tatgtgtatc gccgtctgcc cggtcaagta ctgtatcgac ggctcgggcg
     1321 acaaggtaac gataaacccc gatctgtgca tcggctgcgg tagctgcgtc aaggtctgta
     1381 cgcagaaggc gcgcgtcatc atcgacgatg ctgccaggtt cgtcgaggcc ctcggccgtg
     1441 gcgagaagct cgtcgcggta gtcgcgccgg ccatagccgc gagctacccg ggcgaacacc
     1501 gccgcttcct cggctggctc aagggcctcg gcgtatcggc ctgcttcgac gtcagcttcg
     1561 gcgccgagct gaccgtccgc tcctacctgc gccatatcga ggcggcgcgg cccgagctcg
     1621 tcatcgccca gccgtgcccg gccatcgtca actacatcga gatctacaga cccgagctac
     1681 tcggctacct tgccccggcc gatagcccga tgctccatac catcaagatg atacgggagt
     1741 actatccggc ctatcgcgac cacaagatac tcgtcgtctc gccctgcgcg gccaagaagc
     1801 gcgaattcga ggagaccggc gccggcgact acaacgtgac gctcaggtcg ctcgacgata
     1861 tgctcaagca gcgcaaggtc gacctatcgc ggcagccgga ggccgagttc gagaatagcc
     1921 cggccgagcg cgccgtcctg ttctcgaccc cgggagggct cctgcgaacg gtcgagcgcg
     1981 acctaccgga tgtgcgcgcc gtggccagga agatcgaggg gccggagctg gtctatccct
     2041 acctcgatac gctacccgaa gcgctcggaa agggcgtcaa cccacttctc gtcgattgcc
     2101 tcaattgcga gtacggctgt aacgccggtc cgggaacgct gaaccaggac ctggctcccg
     2161 acgtgatcga gtgggctgtc gaacgccgcg cgaaggaggg ccgcgttgcc tacgccggca
     2221 cgaggggcga cgatcgcgtc gcgcgcaggc gtgtcgagaa ggccctgtcg cgctactgga
     2281 agcccggcct gtacgcgagg acctacgtcg accggtcagc cagctatgcc ctccgtatac
     2341 cgacgaacgc cgagctcgag gccctgtacg ccgatatgct caagaaaggc gccgaggatc
     2401 tgctgaactg cgcggcttgc ggttacaagt cgtgccgggg catggccatc gccatccata
     2461 acggcctcaa taagaaggaa aactgccacc tgttcaaaca gcgggtgatc gagcgggagc
     2521 aacgcatcgt ggacgaggcg tcggtgcggc tccacgagga gatccagcgc atgctcgagg
     2581 acgtgcgtgc gatctcggga gagctgtcgt cgctccatag ccgatccgag gcccagttcg
     2641 aggctatcca ggcctcgtcg accgcggtgc gggagatggt cgacacgctg tcgagcgact
     2701 cgggcatagc ctccggtaag cgcactcaga tcatggcgct cgccggcgcc gccgaggacg
     2761 gggagagcgg catgcgctcg accatggaga cgatacgcca gaccgcggcc gaggtgtcgg
     2821 gcatcggctc gatgatggat gtgatccagg acgtggccga cacgaccaac ctgctcgcta
     2881 tgaacgccgc tatccaggcg gccagggccg gaaccgccgg caagggcttc gccgtggtcg
     2941 ccggcgagat acggaggctc gcggaggcca ctaagagcca agccgacggg ataggcgcgt
     3001 cgctcggctc gatcatcgag cagatagcct cctccgacga cctgatactc cggaccgagg
     3061 cgagcatgca ggcgatgtcg cgtgacgtca gcgtgatggc cgaggagatg tccggcctga
     3121 tcgactcctt ctcagggctg tcgtcgggcg gcgccacggt gatggccggc atcgacgagc
     3181 tcaagacggc caccggcgag gtcaggagca tctacgccaa catctcggtc gagctcgagc
     3241 ggctcttctc gaggataggc gccatcgcgt cgatctcgga acggacccaa gcgaccatct
     3301 cggccatgtc gcgctgaccg acggcccgtc cgaccccctc gcgcccggcc attgacgacc
     3361 ggccggtcgc cctcctataa cggacgcatg aaacgcgacc tgagctccgg cagcgtgctc
     3421 ggcaaccttc tggccatgtc cgtaccgacc atgttcggca tgctcggcca gaccctgtac
     3481 gacgtcatcg acatgatctg gataggccgc atctccggcg aggccgtcgc cggcgtggcg
     3541 ctgttcagct ccatcttctg gctcgtcgag atactcaacg agatcgtggg cgtgtcgtcg
     3601 gtcgcgatga tcacccagag ctacggctcg ggcgacctgg cgcggaccgg ccgcatcatc
     3661 gagcagacca tcgtcttcaa ggcgctcatg gcgatcatcg ccgcgctcgc tctcaacctg
     3721 gtcctcgagc cgctgatccg cttcttctcc gacgatccgg aggtgctcag gagcgcgctc
     3781 gagtacggcc gcgtgcgcat ctacttcctg ccggtcttct tcgccaccta tagctgcttc
     3841 acggcgctcc gctgcaccgg agacccggcc agccagatgt ggatcatgct cggcgcctcg
     3901 gtgctcaata ccgtgctcga cccgatcctg atgttcgaga cggtgcccgg cctgggctgg
     3961 aggggctgcg ggctcggcgt gttcggggcc ggcctcgcga cggtcatatc gatctgcctg
     4021 gccttcgctg tgggcttcgg gctactcgcc cgcggccgcg gcggggtcag gctgacgccg
     4081 gccggcctgt tcaggctcga ccccgcgata gaccgtaagc tcctgacgat aggcctcccg
     4141 agcggcgggg agatgctcgc caggcaggct gccgggctcg tcacgaccaa attcgtcgcg
     4201 gcctacggca cggcggctat cgcggcgctc ggcatcggca accgtctcgg ggggctcgtg
     4261 ttcatgccgc tgttcgggtt gatgtcgggc ggcggcacca tcgtcgggca gaacctgggc
     4321 gccgagaacg tcgagcgggc cgagaagacc gctaaggcgg cctcgctgtt cggggggctc
     4381 ctgatcaccg cgctgatggg cctggcgatc gccttcccgc ggccggtgat ggccgtgttc
     4441 gtcgactccg cggagacgat agaggcgggc gtgtcgatga tacggctgat gggctcgtcg
     4501 ttca
//
LOCUS       DAOPHV010000166         4498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_26061_length_4498_cov_2.737340, whole genome shotgun sequence.
ACCESSION   DAOPHV010000166 DAOPHV010000000
VERSION     DAOPHV010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26061_length_4498_cov_2.737340"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLQ29_05660"
     CDS             <1..194
                     /locus_tag="PLQ29_05660"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPG86169.1"
                     /translation="GRLMRTTLYPKYVELEGRLMPSQLLIVDELNPGERSQMTMTEQS
                     LAPLPDKVFTKAFLEQVNK"
     gene            191..1702
                     /locus_tag="PLQ29_05665"
     CDS             191..1702
                     /locus_tag="PLQ29_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86170.1"
                     /translation="MTASFRILRALPRATMAVLAAAAFAAAAAPGAAAQDSFSFDEDE
                     LFGAEDAVVETVAESAEGSAVRGLLVSESVRIGGSFVGKASGSWTWDDPWGGSFDLAE
                     PDSYGLSPVLSALVFFDGRPTDSTRFYGSVKTAWPFVSSTSVLTGAEYMPESAFPAAE
                     PSVATSSTTLGLPNLEIFELFSDFSWNDSLYLRFGKQTINWGVGYFFSPANVMNLEAI
                     DPFDPEAQLEGPVALRAHYPVPGSQHNLWGYAVFDSANMRPQDTALAAKAEFVVGGWE
                     LGAGGYYKNEEPLRGVLTASGSVGQLSLFGEAALARGSDRTWVTEVSPALAANRFVAT
                     ETDEESPFFKGTAGFMYSHSDSNVTVMGQYLYDGEGYSSDDREARIAEALDAEDAIKA
                     LLAYTVDDVDAAYSGFLKGLILNSGRHYAALSFSKSELLLDDLSFSLFAIGNLSDLSA
                     LLRPSLSYAFFDGLSASLSATFALGFDDGEYVVLYDGRAMALSVAVTLGSGAF"
     gene            1973..3184
                     /locus_tag="PLQ29_05670"
     CDS             1973..3184
                     /locus_tag="PLQ29_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPG86171.1"
                     /translation="MKAAKISDRIWALHADVDSDDRFEGIWPIPHGVSLNSYLVKGEK
                     IALIDLVRDWCGAAANLKAQLADAGVEVSSIDYLILNHLEPDHTGWLAEFKAENPGVE
                     IIATAKGVELVKSFYKVTDGLRAVKSGDELDLGGVSLRFEEIPNVHWPETMVTFEPES
                     GTLFSCDAFGSFGKLGKRVFDDEYYAEEHAFFQGESLRYYANIVGSFSLFVKRAVQKL
                     GPLGIKVIAPSHGIIWRERPQVIIDRYLKYAGYMDGPQEKEICVVWGSMYGNTRKALE
                     ALLKGIEDERVPFSIHRVPDEDVSFVLADAYKSAGIVIAMPTYEYKMFPPMAYVLDIF
                     ERKHVWNRKALRIGSWGWVGGAKKEYEERVAPLKWTHVEPVEWAGAADEATLALLRER
                     GRELAKAVKNG"
     gene            complement(3300..3452)
                     /locus_tag="PLQ29_05675"
     CDS             complement(3300..3452)
                     /locus_tag="PLQ29_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86172.1"
                     /translation="MDAFLEGLKHSSTTVQALGVSAIGLAGVFITLASFFAIIWLAVK
                     AGGKKA"
     gene            complement(3463..>4498)
                     /locus_tag="PLQ29_05680"
     CDS             complement(3463..>4498)
                     /locus_tag="PLQ29_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPG86173.1"
                     /translation="YEPMLLLPIGFGAILVNLPLSVVWEHEGAAGFLKVLYDGGISNE
                     LFPILIFIAVGAMIDFGPLFKNPFMIFFGAAAQLGIFATMLIATLPPFNFSLKEAASI
                     GIIGAADGPTAIYVASKFARDMLGPISVAAYSYMALVPLIQPPVIRLLTTHKERCIHM
                     EYHEAGVPRWLKIAFPIVVTMIAGFVAPISVPLVGSLMFGNLVRESGVLERLSQTAQN
                     ELAYLVTLLLGITIGSSMRADRFLNVQTLMILGMGLVAFVFDTAGGVLFAKLVNLFLP
                     KDRKVNPMIGACGISAFPMSARVVQKMAASEERGNIILMHAIGSNVSGQLGSIVAGGM
                     VLALVPLLVG"
BASE COUNT          792 a         1524 c         1423 g          759 t
ORIGIN      
        1 cgggccgcct gatgcgcacc acgctctacc ccaagtacgt cgagctcgag ggccggctga
       61 tgccgtccca gctgctgatc gtcgacgagc tgaaccccgg cgagcgcagc cagatgacca
      121 tgaccgagca gtcgctggcc ccgctccctg acaaggtctt caccaaggcc ttcctcgagc
      181 aggtgaacaa atgaccgcct cgttcaggat cttgcgcgcg ctgccgcgcg cgactatggc
      241 cgtattggca gccgcggcct tcgccgcggc cgccgccccc ggggcggcgg cccaggactc
      301 gttctcgttc gacgaggacg agctcttcgg agccgaggac gcggtcgtcg agacggtcgc
      361 cgagtcggcc gagggctccg ccgtgcgcgg cctcctcgtc tccgagtcgg tacggatcgg
      421 cggctccttc gtcggcaagg cctcgggctc gtggacctgg gacgacccct ggggcggctc
      481 cttcgacctc gcggagcccg actcctacgg cctatcgccg gtactgtccg ccctcgtatt
      541 cttcgacggc cggccgaccg actcgacgcg cttctacggc tccgtcaaga cggcctggcc
      601 cttcgtctcg tcgacgagcg tgctgaccgg cgccgagtac atgcccgaga gcgcgttccc
      661 ggcggccgaa ccgtcggttg ctaccagctc gacgacccta ggtctgccga acctcgagat
      721 cttcgagctg ttctccgact tctcgtggaa cgattccctg tacctgcgct tcggcaagca
      781 gacgatcaac tggggcgtcg gctacttctt ctccccggcc aacgtgatga acctcgaggc
      841 catcgacccg ttcgaccccg aggcccagct cgaggggccc gtcgccctgc gcgcccacta
      901 ccccgtcccg ggcagccagc acaacctctg gggctacgcc gtcttcgatt ccgcgaacat
      961 gaggccgcag gacacggcgc tcgccgccaa ggccgaattc gtcgtcggcg gctgggagct
     1021 cggcgccggc ggctactaca agaacgagga gcccctccgc ggcgtgctga ccgcctccgg
     1081 ctcggtcggg cagctgtcgc tcttcggcga agccgcgctg gcgcgcggct cggaccggac
     1141 ctgggtcacc gaggtgtccc cggccctcgc cgcgaaccgt ttcgtagcca ccgagaccga
     1201 cgaggagtcg ccgttcttca agggcaccgc cggcttcatg tacagccata gcgactcgaa
     1261 cgtcaccgtc atgggccagt acctctacga cggcgagggc tactcgagcg acgaccgcga
     1321 ggcgcgcata gccgaggccc tcgacgccga ggacgcgatc aaggcgctcc tcgcgtacac
     1381 ggtcgacgac gtcgacgcgg cgtactccgg cttcctcaag gggctgatcc ttaactccgg
     1441 caggcactac gcggcgctga gcttctccaa gtcggagctg ctcctcgacg acctgtcgtt
     1501 ctcgctgttc gcgatcggca acctgtcgga cctatcggcc ctcctccggc ctagcctgtc
     1561 gtacgcgttc ttcgacgggc tgtcggcgtc gctgtcggcc accttcgcgc tcggcttcga
     1621 cgacggcgag tacgtggtac tctacgacgg ccgcgcgatg gccctgtccg tcgccgtcac
     1681 cctaggctcg ggcgccttct aagcgaaggc agcgacgcgc ttgaaccacg gaggcacggg
     1741 gacacggagg agcaggggag agaaggagaa gacgagccta ggcagcttca tccaatcctg
     1801 tagtaccata cgcaatcctc tccgtgcctc cgtgtctccg tggtagtctt catctactcg
     1861 tcccgtatta ttttctcctt gtgctattcg ggctttttgg cctagtatcg cttacagttc
     1921 atggcgcggc cggatggccg cgcctagtcg cgcaaaaagg agccgcacga ggatgaaagc
     1981 tgccaagatc agcgatcgta tttgggccct gcacgcggac gtcgattcgg acgaccgctt
     2041 cgagggcatt tggccgatcc cccacggggt ttcgctcaat tcatacctgg tcaaaggcga
     2101 gaagatagcg ttaatcgacc tcgtgaggga ctggtgcggg gctgcggcca acctgaaggc
     2161 ccagctggcc gacgccggcg tcgaggtctc gagcatagac tacctgatac tcaatcacct
     2221 ggagccagac cacaccggct ggctggccga gttcaaggcc gagaaccccg gcgtcgagat
     2281 catcgccacg gccaagggcg tcgagctggt caagagcttc tataaggtca cggacggtct
     2341 gcgcgcggtc aagtccggcg acgagctcga cctcggcggc gtatcgctgc gcttcgagga
     2401 gattccgaac gtccactggc cggagacgat ggtcaccttc gagcccgagt caggcacgct
     2461 gttctcctgc gatgccttcg gcagcttcgg caagctcggc aaacgcgtgt tcgacgacga
     2521 gtactacgcg gaggagcatg ccttcttcca gggcgagagc ctgcgctact acgccaacat
     2581 cgtcggtagc ttcagcctgt tcgtcaagcg agccgtacag aagctggggc ccctcggcat
     2641 caaggtcatc gcgccgagcc acggcatcat ctggcgcgag cgtccccagg tcatcatcga
     2701 ccgctacctg aagtacgccg gttacatgga cggcccccag gagaaggaga tctgcgtcgt
     2761 ctggggctcg atgtacggca acacccgaaa ggcgctcgag gccctgctca agggcatcga
     2821 ggacgagcgc gtgccgttct cgatacaccg cgtgcccgac gaggacgtgt ccttcgtgct
     2881 agccgacgcg tacaagagcg ccggcatcgt catcgcgatg cccacctacg agtacaagat
     2941 gttcccgccg atggcctacg tgctcgatat cttcgagcgc aagcacgtgt ggaaccgcaa
     3001 ggccctgcgc atcggctcct ggggctgggt cggcggcgcc aagaaggaat acgaggagcg
     3061 ggtcgccccg ctcaagtgga cccacgtgga gccggtcgaa tgggccggcg ccgcggacga
     3121 ggccaccctc gccctgctgc gcgagcgcgg ccgcgagctg gccaaggccg tcaagaacgg
     3181 ctaagcccgc ccgctagcgt agttgctatg acgaggcccg cgccggacac ccggcgcggg
     3241 cctcgtcata gcgggcggag ccgggagtcg tctccctcca ggggtcccgc gaccgggcgt
     3301 tacgccttct tgccgccggc cttgaccgcc agccagatga tggcgaagaa cgacgcgagc
     3361 gtgatgaaaa cgccggccag ccctatggcg ctgacgccaa gggcctggac agtcgtcgac
     3421 gagtgcttga gaccctctaa gaaagcgtcc atcgttaccc ccttagccta cgagcagcgg
     3481 cacgagcgcc agcaccatgc cgccggccac gatcgacccg agctggcccg acacgttcga
     3541 ccctatcgcg tgcatcagta tgatgttgcc gcgctcctcg ctcgccgcca tcttctgcac
     3601 cacgcgagcc gacatcggga aggccgagat gccgcaggcg cctatcatcg ggttgacctt
     3661 cctatccttc ggcaggaaca ggttgaccag cttggcgaac agcacgccgc cggcggtgtc
     3721 gaacacgaag gcgacgagac ccatgccgag tatcatcagc gtctgcacgt tcaggaagcg
     3781 gtccgcgcgc atgctcgagc ctatcgttat gcccaggagc agggtgacga ggtaggccag
     3841 ctcgttctgc gccgtctgcg acaggcgctc gagcacgccg gactcgcgta ccaggttgcc
     3901 gaacatcagc gatccgacga gcggtaccga tataggcgcc acgaagccgg ctatcatcgt
     3961 caccacgatc gggaaggcta tcttgagcca cctgggcacg ccggcctcgt ggtactccat
     4021 atgaatgcac cgttccttat gggtcgtcag gagccgtatc accggcggct ggatgagcgg
     4081 tacgagggcc atgtacgagt aggcggccac cgatatgggc ccgagcatgt cccgcgcgaa
     4141 tttgctcgcc acgtagatgg cggtcgggcc gtccgcggcg ccgatgatgc cgatgctcgc
     4201 cgcctccttg aggctgaagt tgaacggcgg cagcgtcgct atcagcatcg tcgcgaagat
     4261 gccgagctgg gccgccgcgc cgaagaagat catgaacggg ttcttgaaca agggcccgaa
     4321 gtctatcatc gctccgaccg cgatgaagat gaggatgggg aacaactcgt tggagatacc
     4381 gccgtcgtag agcaccttga ggaacccggc cgcgccctca tgctcccaca ccaccgacaa
     4441 gggcaggttg acgagaatgg ccccgaagcc tatgggcagg agcagcatcg gctcatat
//
LOCUS       DAOPHV010000167         4470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_26276_length_4470_cov_3.964666, whole genome shotgun sequence.
ACCESSION   DAOPHV010000167 DAOPHV010000000
VERSION     DAOPHV010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26276_length_4470_cov_3.964666"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PLQ29_05685"
     CDS             <1..774
                     /locus_tag="PLQ29_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG86174.1"
                     /translation="TDRGGRIYLATGTVRYDEPVMGAIEPVPAGDWSYIEVVDEGPGI
                     PPELLDKVFQPFFTTKPADRGTGLGLSTVAGIVRQAEGRIALVSKPGAGTTMRLLFPR
                     VQGGQPREEAGAHDRGAVRARHGSLPAGAYRPRILLVEHDDSVRAILEALMERAGYAV
                     FSATHPGEALLIAENMANGPDLLVADDELPLMGGVELAGRLRAGGRAVPALYLRAADG
                     NHEDRPAVDSATGAEAFLDKPFTEDELMDAMEGLLPRRS"
     gene            complement(771..1481)
                     /locus_tag="PLQ29_05690"
     CDS             complement(771..1481)
                     /locus_tag="PLQ29_05690"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG86175.1"
                     /translation="MGYEVKGLAKSFGELRVLGGLDLEFPAGRVTVVLGPSGCGKTSL
                     LNILAGLDEDYSGEVLGFGPGKASYVFQEDRLMPWMSAMDNVALVLRRFMDRDHAMAT
                     AEEALAAVGLHGSRGSKPGAMSGGMRRRVALARAFAYPSEVLLLDEPFSSLDLRTRIS
                     VMDLFLDLRERDGRSAVVVTHDVREAIYLGSRIATLSDKPAKTRDVFDLTLAKEDRGF
                     ASRAAASFEARLYESILS"
     gene            complement(1466..2227)
                     /locus_tag="PLQ29_05695"
     CDS             complement(1466..2227)
                     /locus_tag="PLQ29_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPG86176.1"
                     /translation="MMPGTRTSRAKADAVLAVAVLAAVWKLGSMAIGADIVLPAPERV
                     LSTLLRVASDDGFAAALSATALRGLASFGISMALGVSLGFAAGLSDRCRAMLAPALTV
                     TRATPVLAIILLAMIWFPSGIVPVFSAVVMAFPVAVADVAAGVRSTDPRLLDMARSFG
                     VPARDIAARIRFPSAAPHLASAARNVIGLSWKVVVAGEVLSQPARAVGTGMQNARVML
                     ETAEVFAWVTVGILLCAVSDALFDLLSRRLSWATK"
     gene            complement(2196..3044)
                     /locus_tag="PLQ29_05700"
     CDS             complement(2196..3044)
                     /locus_tag="PLQ29_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPG86177.1"
                     /translation="MIGLFENPPALPQAATASFEAVPSADAMAAKLLSRELDAAALPV
                     NLAAKLYNSGLGYRLVAVVGNGMVKLVTTDPSLSSPADLRGRELYVAGQGATPEFLMR
                     TVLIEVGLDPSRDLRLAFNMPYPEMAASLVAGRISSAVLPEPFATLALSGNKNAVVPF
                     SLTELWKRATGQDDYPMTVFVVRSTLIDERPAAVAALAAAYEASIRSTLADPAAAGTL
                     VEKHDMGLKAPIAAAAIPACAFTFVAAPEARPAVEALLSVFLASVPASVGGKLPDDAW
                     YADLAR"
     gene            3327..3794
                     /locus_tag="PLQ29_05705"
     CDS             3327..3794
                     /locus_tag="PLQ29_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HPG86178.1"
                     /translation="MRCDACGSIDSVFFIKPDGAGGEQRLCRRCALARGYAVGGDGVL
                     GAIVDPDPGEGPEPVCRDCGWTAERLRATGRLGCSSCASTFRSLVSAMLARAGARGAY
                     DGKVPRTAAPARDADGSRAAMTLSLERAVAREDFETAASIRDRLRAAAAKARP"
     gene            3791..>4470
                     /locus_tag="PLQ29_05710"
     CDS             3791..>4470
                     /locus_tag="PLQ29_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86179.1"
                     /translation="MTLHGPASMPPPRGDHPEYDVVVQSFVQADRNLVGTRFPHAMRP
                     GEAGELRACLGAAARACAYAVARVGDLDAQCRSNLAERELYSRPYLLDDENLAALRVD
                     SAAWLAFNDTNHLSLRSALPGLALEAAWDLVSAADDQISACLGDRPWAFDTDMGYIMS
                     EASRCGSGLSARITVHAPALVLSGLAETAFKRAMEAGFVVSGSYSSLAASGGSLFDLS
                     LPYASRGP"
BASE COUNT          741 a         1478 c         1562 g          689 t
ORIGIN      
        1 accgaccggg gcggacgcat ctacctcgcg accggaaccg tccggtacga cgagccggtc
       61 atgggcgcca tcgagccggt gccggccggc gactggtcgt acatcgaggt cgtcgacgag
      121 gggccgggca tcccgccgga gctactggat aaggtgttcc agccgttctt caccacgaag
      181 cccgcggatc ggggcacggg tctcggcttg tccaccgtcg ccggcatcgt ccgccaggcc
      241 gaggggcgga tagccctggt ctccaagccg ggcgccggta cgacgatgcg cctcctattc
      301 cccagggtgc agggcggcca gccgcgggaa gaagcggggg cgcacgatcg gggcgccgta
      361 cgcgcgagac acggatcgtt gccagcgggg gcctatcggc ccaggatact cctcgtcgag
      421 cacgacgact cggtccgggc catactcgag gcgctgatgg aaagggccgg ctacgcggtg
      481 ttctcagcta cccatcccgg cgaggccctg ctcatcgccg aaaatatggc taacggaccg
      541 gacttgctcg tcgccgatga cgagctgccg ctgatgggcg gggttgagct ggctggcagg
      601 ctgcgcgccg ggggcagggc cgtaccagcc ctgtacctca gggcggccga cgggaaccac
      661 gaggaccggc cggccgtcga ttcggctacg ggcgcggagg cgttcctcga taagccgttt
      721 actgaggacg agctcatgga cgccatggag ggactgctcc ctcgccgctc ctaggacagt
      781 atggattcgt acaggcgcgc ctcgaaggag gccgcagcgc gactggcgaa gccgcgatcc
      841 tccttggcca gcgtcaggtc gaagacatcc ctggtcttgg cgggcttgtc cgacagcgtg
      901 gctatcctcg agcccaggta gatagcctcg cgtacgtcgt gggtcacgac gacggcgctg
      961 cgtccgtcgc gctcgcgcag atcgaggaat agatccatca cggagatgcg cgtacgcagg
     1021 tcgagcgacg agaagggctc gtcgagcagc aacacctccg acgggtaggc gaaggccctg
     1081 gcgagcgcta ccctgcgccg catgccaccg gacatggccc cgggtttcga gccgcgagag
     1141 ccgtggagcc ctacggccgc cagggcctcc tcggccgtcg ccatggcgtg gtcgcggtcc
     1201 atgaaacgcc tgagcaccag cgctacgttg tccatagcgc tcatccaggg catcaggcgg
     1261 tcttcctgga atacgtagct ggcctttccg ggtccgaacc cgagcacctc tccggaatag
     1321 tcctcgtcga gaccggcgag tatgttgagg aggctcgtct tgccgcatcc cgacgggccg
     1381 agcacaaccg tgacacggcc ggccgggaat tccaggtcga gcccgccgag tactcgcagc
     1441 tcgccgaagg acttcgcgag gccctttact tcgtagccca cgagagcctc ctggacagca
     1501 ggtcgaacag agcgtcgctc accgcgcaga gcagtatccc gacggtcacc caggcgaata
     1561 cctcggcggt ttctagcatg acgcgggcgt tctgcatgcc cgtgcccacg gctcgggccg
     1621 gctggctcag tacttctccg gcgacgacga ccttccaaga taggccgatg acattcctgg
     1681 cggccgaggc gagatgcggc gcggccgacg gaaagcgtat cctggccgct atgtcgcggg
     1741 ccggcacgcc gaagcttcta gccatgtcga gcaggcgagg gtcggtggac cggacgccgg
     1801 ctgccacgtc ggcgacggcg acggggaagg ccatcacgac cgccgagaag accggtacga
     1861 ttcccgatgg gaaccagatc atcgccagca ggatgatggc caggaccggg gtggcccgcg
     1921 tgacggtcag cgccggggcg agcatggccc tacagcggtc agacagcccg gctgcgaagc
     1981 cgagcgagac gcccagggcc atcgatatgc cgaacgaggc gaggccccgg agcgccgtag
     2041 ccgacagggc ggcggcgaag ccatcgtcgg acgcaacgcg gagcagggtc gataggacgc
     2101 gctcgggcgc cggcagtacg atgtcggcgc ctatggccat cgagcctagc ttccagaccg
     2161 ccgcgagtac ggcgacggcc aggacagcat ccgctttagc gcgcgaggtc cgcgtaccag
     2221 gcatcatccg gcagcttccc tccgaccgac gcgggtaccg aggcgaggaa caccgatagg
     2281 agcgcctcga cggccggcct ggcctcgggc gcagcgacga aggtgaaggc gcaggccggg
     2341 atggcggccg cggcgatggg cgccttgagc cccatgtcgt gcttctcgac cagggtcccc
     2401 gcggcggcgg gatcggccag cgtcgaccgt atcgatgcct cgtaggcggc cgcgagggcg
     2461 gctacggccg cagggcgctc gtcgatcagc gtggatcgca cgacgaatac cgtcatcgga
     2521 tagtcgtcct ggccggtagc gcgcttccaa agctccgtca gggagaaggg tacgaccgcg
     2581 ttcttgttgc cggacagggc cagcgtggcg aagggctcgg ggagcaccgc gctggagatg
     2641 cggccggcta ccaggctggc cgccatctcg ggatacggca tgttgaacgc tagccgcagg
     2701 tcgcgcgatg ggtcgaggcc gacctcgata agcacggtcc gcatcaggaa ctccggcgtg
     2761 gcgccctggc cggcgacgta tagctcgcgg ccgcgcaggt cggcgggcga cgataggctc
     2821 gggtcggtgg tgacgagctt gaccatgcca ttgcccacga cggccacgag cctgtaaccg
     2881 aggccggagt tatagagctt cgctgccagg ttgacgggca gggccgcggc atccagctcc
     2941 ctcgatagaa gcttggctgc catcgcgtcg gccgaaggca cggcttcgaa ggaggctgtc
     3001 gccgcctggg gcagggccgg gggattctcg aacaggccta tcataccgat gcccgaaggt
     3061 cccttgatag cgccgacccg gaggacgagt ggctcgagcg gggttgcctg ggagacctcg
     3121 cgggaaggtt ctttgccgcc aagggcgaag gcggaagagg ctaacgatag aaccgctaaa
     3181 gcggcgatac aacgacgatg catagacttc ctttcgacgg catggccgtc gactacgatg
     3241 atatcgtcca gcctacgcaa gggcaagcct ggccgcttca ggcgatggcg ctttcatcta
     3301 gcccgcattt agcatatagt aataccatgc gatgcgatgc ctgcggttcg atcgacagtg
     3361 tcttcttcat caagccggac ggtgctggag gcgaacaacg cctctgcagg cgctgcgcgc
     3421 tggcccgcgg ctacgccgtc ggcggcgacg gcgtactagg cgccattgtc gatccggatc
     3481 ctggcgaggg gccggagccc gtctgccgcg actgcggctg gaccgcagag cggcttcggg
     3541 ccaccggaag attaggctgt tcttcctgcg cctcgacgtt caggagcctg gtatcggcca
     3601 tgctggcccg tgccggcgct cgcggcgcgt acgacggtaa ggtgcccagg acggcggcgc
     3661 cggcccgcga cgccgatgga tcgcgggcgg ccatgacgct atcgctcgag cgggccgtcg
     3721 cccgcgagga tttcgaaacc gcggcctcca ttcgagaccg gctccgcgcc gccgcggcta
     3781 aggctaggcc gtgacgctgc acggtcccgc ctcgatgcca cctccgcgcg gggatcatcc
     3841 cgagtacgat gtcgtcgtcc aatccttcgt ccaggcggat cgcaacctgg tcggaacacg
     3901 ctttccccac gccatgaggc cgggcgaagc cggtgagctg cgcgcctgcc tcggcgccgc
     3961 cgcgcgtgcc tgcgcttacg cggtcgcgcg ggtcggtgac ctcgacgccc agtgccgctc
     4021 gaacctcgcg gaacgggaac tgtactcgcg gccatacctg ctcgatgacg agaacctagc
     4081 cgccctgagg gtcgatagcg ccgcctggct ggcgttcaac gacacgaatc acctatccct
     4141 gcgctcggcc ctgccgggcc tggccttgga ggccgcgtgg gacctcgtat cggcggctga
     4201 cgaccagatc tccgcttgcc tcggcgaccg tccctgggcc ttcgatacgg atatgggcta
     4261 catcatgtcc gaggcttcgc gttgcggtag cggcttatct gcacgcatca ccgtgcatgc
     4321 gcccgccttg gtcctgtcag gactggcgga gaccgcgttt aagcgcgcca tggaggcggg
     4381 cttcgtcgtg tccggctcct actcgagcct ggccgcctcc ggaggctcgc tgttcgacct
     4441 gtccttgccg tacgcaagcc gcgggccgga
//
LOCUS       DAOPHV010000168         4432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_26634_length_4432_cov_3.524560, whole genome shotgun sequence.
ACCESSION   DAOPHV010000168 DAOPHV010000000
VERSION     DAOPHV010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26634_length_4432_cov_3.524560"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PLQ29_05715"
     CDS             complement(<1..394)
                     /locus_tag="PLQ29_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86180.1"
                     /translation="MVLIHSRAKRIHGQVIASVAAIVALALLAACENPMADLVAEARV
                     IAVSPAISVVFSSDTAAIDSGSTRDLGTITYSTDESTALLYTVAIKNSGHATLSVDTG
                     AVALSAPEGQAAGVFSIRSLPDATIGVGE"
     gene            complement(385..2472)
                     /locus_tag="PLQ29_05720"
     CDS             complement(385..2472)
                     /locus_tag="PLQ29_05720"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG86181.1"
                     /translation="MRTPPTHRVVRKAILAPWLVAAMALSAPDAAAAPSGLVTLTASP
                     MVTVPITSGDFGPNDGLAAAFGGSLGLEYALPTRFPLALRLSGGFSSGGLLPVDGVEV
                     AGRLSELSVLAGAGTGLALRPSLRLRGFLDGGMAIGRLDSGDGVPYASARAGTGLELD
                     LAEGFSARLDAAWTYMSGLYGGFGATLGVAYALPAPGSGGDRLLEFASADLGEVFPIF
                     RSYYDDNPIGTVRINNASRKTARDVKVSFVIRQYMDAPKECAVIERLAPGKSVDVPIY
                     GLFNDSILSVTEATKAGAEITVEYGSGTTQTKTVTVVVYDRNALTWVDDRHAAAFVSS
                     KDPWVLDLSGNISAAVKDSRNPELPDSVQSAVAFHEGLRVYGISYVLSPNRPFATGAV
                     DAAAVDSLKFPRQTLGYRAGDCADLTVLYASFFEAAGIQTAFITVPGHIFVAFDSGLS
                     VVDAAARSMDERELVVIDGRVWIPIETTMRDSGFVEAWRAAARQWREARSGGAAGFHP
                     VHEAWKTFPPVGLPADGSSAAPPSAKAVKTAFVAELDRLVSLELKARLERLGPRPTSG
                     AARFLNERGVLYARYGRYDDAERDLRAAAKDQYQPAIVNLGNVAFLRGDYKAAYDRFD
                     QAAEDAPDNARLIVSMARTAAALGRSDEVAALIARVRAIDPATADRYASLVGASASGT
                     RAAQAGKEETIWF"
     gene            complement(2566..3669)
                     /locus_tag="PLQ29_05725"
     CDS             complement(2566..3669)
                     /locus_tag="PLQ29_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPG86182.1"
                     /translation="MDAEEAMADGLYDFLCGAFGLSFIAIGAGFIVEPDGERVRKAFG
                     ALFLSLGAAYALSWLSGFWLAPRPIDSLLLLAIVFAISQSLFEITLFVFGDEAVKGSR
                     RKVYLIGAAWSATLWLLPFLDLAFGLGTLGASVEDGQSLAPFHLISTVGVYLWPVAIT
                     VISLVAGKRHLADFPREPGAAWSFLGAFALVIVALGVIGVSMAASSTLGYRLGHLSLM
                     LMMLAWHLYYRARPDAFKRARKEIQDRHEQRRALGPEETAAIAARLDRAVLAGALNES
                     AELNLSALAKRLRLPAYKLSAYLNGELGMSFPAWLNATRVERVKRLLVERPGDSILDI
                     AFETGYASKTVFNNQFRKLVGMSPSEYRESHRA"
     gene            complement(3717..>4432)
                     /locus_tag="PLQ29_05730"
     CDS             complement(3717..>4432)
                     /locus_tag="PLQ29_05730"
                     /inference="COORDINATES: protein motif:HMM:NF015164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HPG86183.1"
                     /translation="ALGEAERGESAALERYRAAVSMLVGRDVSALDAPGVARACVESV
                     AESSVDGAAAPLFWAAMLGPAGAFAYRAVNTMDSMFGHKDERYLRFGLVPARADDVAN
                     WLPARLSSTLACLLAPVAGGSPRAAFRSFARWRLAHESPNAGHPEAAYAGALGLRLGG
                     PSRYAEGVIGKPWINPTGGEAASTHVRGAVRLMYAQALLSAASFVALRIALWDALWVA
                     LRGASRAIIASIATILASA"
BASE COUNT          712 a         1457 c         1622 g          641 t
ORIGIN      
        1 attcgccgac gccgatagtc gcgtcgggga ggctccgtat cgagaagacg ccggctgctt
       61 gcccctctgg cgcggatagc gcgacggcgc ccgtatcgac gctgagcgtc gcgtggcccg
      121 aattctttat cgcgaccgta tagagcagcg ccgtcgattc gtcggtgctg taggttatgg
      181 tcccgaggtc acgcgtggag cccgagtcga tcgccgccgt gtcggacgag aagacgacgg
      241 aaatagcggg agatactgcg atgaccctag cctcggcgac gagatccgcc atcggattct
      301 cgcaggcggc caggagggcg agcgcgacga tggcggcgac gcttgcgatt acttggccgt
      361 ggatgcgctt tgcgcgcgag tgaatcagaa ccatatcgtc tcctccttgc cggcctgggc
      421 ggcgcgggta ccggacgcgg acgcgccgac gagcgaggcg tacctgtccg cggtggccgg
      481 gtcgatggcg cggacccggg cgatcagcgc ggctacctcg tcgctacggc ccagggcggc
      541 ggcggtcctg gccatgctga cgatcaggcg cgcgttgtcc ggggcgtctt cggcggcctg
      601 gtcgaagcgg tcgtacgcgg ccttgtagtc gccgcggagg aaggccacgt tgccaaggtt
      661 gactatggcg ggctggtact ggtccttcgc ggcggccctg aggtcgcgct cggcgtcgtc
      721 gtacctgccg tacctggcgt acagtacgcc gcgctcgttc aggaagcgcg cggctccgga
      781 cgtcggccta gggccgagcc gctcgagcct ggccttgagc tcgagcgata cgagcctgtc
      841 gagctcggcg acgaaggccg tcttgacggc cttggcggag ggcggcgcgg cgctcgagcc
      901 gtcggccgga aggcctacgg gcgggaaggt cttccaggcc tcgtggacgg gatggaaccc
      961 ggccgcgccc ccagagcgag cctcgcgcca ttgcctggcc gcggcgcgcc aggcctccac
     1021 gaagcccgag tccctcatgg tcgtctctat gggtatccat accctgccgt cgatcacgac
     1081 gagctcgcgc tcgtccatgg agcgggcggc cgcgtcgacg accgacaggc cggagtcgaa
     1141 ggccacgaag atgtggccgg gcacggtgat gaacgcggtc tggatgccgg cggcctcgaa
     1201 gaacgaggcg tacagtaccg tcaggtcggc gcagtcgccg gcgcggtagc cgagcgtctg
     1261 cctggggaac ttgaggctgt ccacggcggc ggcgtcgacg gcgccggtcg cgaagggccg
     1321 gttggggctc aggacgtagc tgatgccgta gacgcggagg ccctcgtgga aggctacggc
     1381 gctctggacg ctatcgggta gctcggggtt gcgcgagtcc ttgacggcgg cgctgatgtt
     1441 gcccgacagg tcgagcaccc aaggatcctt gctcgagacg aaggcggcgg cgtgccggtc
     1501 gtcgacccag gtgagcgcgt tgcggtcgta cacgacgacg gtgaccgtct tcgtctgggt
     1561 cgtgccggat ccgtactcca cggtgatctc ggcgccggcc ttggtcgcct cggtgaccga
     1621 cagtatgctg tcgttgaaga gcccgtagat aggcacgtcg accgacttcc cgggcgccag
     1681 ccgctcgatg acggcgcatt ccttgggggc gtccatgtac tgcctgatga cgaagctcac
     1741 cttgacgtcc ctggcggtct tcctgctcgc gttgtttatg cggacggtgc cgatcgggtt
     1801 gtcgtcgtag tacgagcgga agatcgggaa gacctcgccg agatcggccg acgcgaactc
     1861 gagcaggcga tcgccgccgg atcccggagc cggcaaggcg taggcgacgc cgagcgtcgc
     1921 gccgaagccg ccgtacaggc cggacatata ggtccaggcg gcgtcgagcc tcgcggagaa
     1981 gccttcggcg aggtcgagct cgagcccggt cccggcgcgc gccgaggcgt agggcacgcc
     2041 gtcgcccgag tcgagccggc cgatggccat accgccgtcc aggaagccgc gcagcctgag
     2101 cgacggcctg agcgcgaggc ccgtcccggc gccggccagc acggacagct cgctgagcct
     2161 gccggcgacc tcgacgccgt cgaccgggag tagcccgccc gacgagaagc cgcccgatag
     2221 cctgagcgcc agcgggaagc gggtcggtag cgcgtactcg agcccgagcg agccgccgaa
     2281 ggccgcagcc aggccgtcgt tcggcccgaa gtcgccggag gtgatcggca cggttaccat
     2341 cggggacgcc gtcagcgtca cgaggcccga aggagcggcg gccgcgtccg gcgcggacag
     2401 cgccatggcc gcgaccagcc agggggccaa tatggctttt cggacgactc tatgggtcgg
     2461 gggtgtacgc atcgcgcgcc tccatcgtaa tcaggctgaa tccgtaacgg atatatagca
     2521 tccatatacg gcgcggtcgt cctgattcga tgaaacggta cgatcctagg cccggtgcga
     2581 ctcccggtac tcgctcgggc tcatgccgac gagcttgcgg aactggttgt tgaagacggt
     2641 cttggaggcg tagccggtct cgaaggcgat gtccaggatg ctgtcgccgg gccgctcgac
     2701 gagaagcctc ttgacccgct cgacgcgcgt cgcgttgagc caggccggga agctcatgcc
     2761 cagctcaccg ttcaggtatg ccgacagctt gtaggccggt agcctcagac gcttggctaa
     2821 cgcgctcagg ttgagctcgg ccgactcgtt gagggctccg gccaggacgg cccggtcgag
     2881 ccgcgccgcg atggccgccg tctcctcggg gccgagcgcc cgacgttgct cgtgcctgtc
     2941 ctggatctcc ttgcgcgcgc gcttgaacgc gtccggcctc gcccggtagt ataggtgcca
     3001 cgcgagcatc atcagcatca gggacaggtg gccgagacgg tagccgaggg tcgacgaggc
     3061 ggccatcgat acgcctatga cgccgagcgc gacgatgacg agcgcgaagg cgcccaggaa
     3121 cgaccacgcc gcgccgggct ccctcgggaa gtccgccaga tggcgcttgc cggcgactaa
     3181 ggagataacc gtgatggcca cgggccatag gtacacgccg accgtgctga tcagatggaa
     3241 cggggcgagc gactggccgt cctcgacgct cgctccgagc gtcccgaggc cgaacgcgag
     3301 gtcgaggaac ggcagcagcc acagagtggc cgaccaggcc gcgcctatca ggtatacctt
     3361 ccggcgggaa cccttgaccg cctcgtcgcc gaagacgaac agggtgatct cgaagagcga
     3421 ctggctgatc gcgaagacta tggccagcag gagcagcgag tcgatgggcc tcggcgccag
     3481 ccagaagccg ctgagccagc tgagggcgta agccgccccg agggacagga acagggcgcc
     3541 gaaggccttg cgcacccgct ccccgtccgg ctccactatg aaaccggccc cgatggcgat
     3601 gaacgaaagg ccgaaagccc cgcataagaa gtcgtacagg ccgtcggcca tcgcttcctc
     3661 cgcgtccatg atcgctcggg atgcgccgag cggtcaacca tgaatcgacg gcgcgcctac
     3721 gcggacgcga ggatcgtcgc gatggacgct atgatcgctc gcgacgcgcc tcgtagggcg
     3781 acccagaggg cgtcccagag ggcgatccga agggcgacga aggaggcagc cgagagcagg
     3841 gcctgcgcgt acatgagcct gacggccccg cggacgtggg tcgaggcggc ctcgccgccg
     3901 gtcgggttga tccatggctt gccgatgacg ccctcggcgt agcgggacgg gccgccgagc
     3961 ctgaggccga gcgctcccgc gtaggccgcc tcgggatggc cggcgttcgg gctctcgtgg
     4021 gccagccgcc agcgggcgaa cgagcggaac gccgcgcgcg gcgacccgcc ggcgaccggg
     4081 gcgaggaggc aggcgagcgt cgacgacagg cgggccggca gccagttcgc gacgtcgtcg
     4141 gcgcgggccg gcacgaggcc gaagcgtagg tagcgttcgt ccttgtggcc gaacatagag
     4201 tccatcgtgt tgacggcgcg gtacgcgaag gcgccggccg gcccgagcat ggccgcccag
     4261 aacagcgggg cggcggcgcc gtcgaccgag ctctccgcga cgctctcgac gcaggcgcgc
     4321 gcgacgcccg gcgcgtcgag ggccgatacg tcgcggccga ccagcatcga tacggcagct
     4381 cggtaccgct ccaaggcggc tgactcacca cgctcggcct caccgagcgc cc
//
LOCUS       DAOPHV010000169         4425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_26692_length_4425_cov_3.754005, whole genome shotgun sequence.
ACCESSION   DAOPHV010000169 DAOPHV010000000
VERSION     DAOPHV010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26692_length_4425_cov_3.754005"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="PLQ29_05735"
     CDS             <1..738
                     /locus_tag="PLQ29_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPG86184.1"
                     /translation="LLALTSLTIALAYEPLAYACRFLPARLRRERVAWVALAAMDRFV
                     ACARAYIGMRLRDERAGFDAPERCIVVSNHQSLLDIPVLMHYFRAERRLLFVAKKELG
                     YGIPLISSVLRVGGHALIDRGSNPTRAMRELGAFARRSGAPGFWPSVFPEGTRSKDGS
                     LGKFHTAGLRRIADLAPAPLLVVAVDGGYKATKLSSLLTGSVGFEYRVKALGLIELEP
                     GKKGVQEALEAARSLIARQLEAWRAEA"
     gene            complement(832..1002)
                     /locus_tag="PLQ29_05740"
     CDS             complement(832..1002)
                     /locus_tag="PLQ29_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014342390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86185.1"
                     /translation="MGQVSKNSRTSSTTHHFYCPCGGEVKMKTLFEDGRVKNVAECEK
                     CKRTERRPSDFS"
     gene            complement(1296..2666)
                     /locus_tag="PLQ29_05745"
     CDS             complement(1296..2666)
                     /locus_tag="PLQ29_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG86186.1"
                     /translation="MSFVIILIVFFGIAVGVLSFFLIRLIVMPKRLSAVAELIKQGRY
                     QAATKAVKSMLAKEPRNAAAHYYLGKIYLAEGKPELALMEFKTVGDIGQFGHEIPETE
                     FRRLIADLYERFGQLEEALKEYILLAKGDPRNAEYFYQCSRIFDERGKADVATKYARK
                     AVELDPRHGKAHYMLGVILYKTKHPLEAKAEFELGLKFDPSNYDSYYYLGKLMKESND
                     YTGALLAFERAQKSPALKIKALVERGGTYMSQGSYDNAAIELERAVKLAKDDGSSESL
                     YGRYFLSLCYEKMKNIDKAIEQWEKIYAKKPQFRDVAEKLTQYQEYRTDDRIKDYLTC
                     GQDDFIELCKLTAMNGLNLAVRDVIPTPNGVDFITVENESDKWLGTKKMPRLLRFLRV
                     SEQLDDSSIRSLLDNMKKLGIIRGVIVTSSGFNRSAIEFAENRSVELYHKEQLQELLK
                     KASASS"
     gene            2838..3575
                     /locus_tag="PLQ29_05750"
     CDS             2838..3575
                     /locus_tag="PLQ29_05750"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG86187.1"
                     /translation="MLRRGFYRRATPGRFVARLALAFALGTAAVGASELSDRGIGLFM
                     ENKPQEAAPVLELAAKEAATDEKVFLYLGIAYQQLGRWDDAIAAFRRGLAASALYRHQ
                     FLFNIANSFFAQGKNAFALEYYDQALALRGDYAQAYLNRANAKMRLADYPGASADYSL
                     YLSLEPGSSQASEIRRLLDLLGSKAEAARTAAAEAEARRLAEEQARKALLDEVARSLL
                     EAAEATTNLSAGSGDVQGYESDLSLDD"
     gene            3618..>4425
                     /locus_tag="PLQ29_05755"
     CDS             3618..>4425
                     /locus_tag="PLQ29_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86188.1"
                     /translation="MTDKEKKRFVYIGIAALLALAAIAAIVLALGAGGHSGERERTNM
                     LSLAGEYIDRGDFDRALDILDRLIIKDSSDAEARSLQDLALARKASAKAAAEEAAKAG
                     SGEAGALAQSLGELGKSLERTASTVAQSATRPSQPAAGSSAGSADDSAAAQAAARKAE
                     EDALAKAQSEAEAARRRAAEEELSRKSAELRAKMEAINALVKAGRQSISAGDYAAARS
                     SFDEAVSSLPSGEAKFSAQTYAEIAEGYYDGYRRDPTGAEGLESVKQAQRV"
BASE COUNT          768 a         1385 c         1458 g          814 t
ORIGIN      
        1 ctgctagccc tgacctcgct gaccattgcc ctggcgtacg agcctctcgc ctacgcgtgc
       61 cgtttcctgc ccgcgcgcct tcgccgcgag cgggtcgcct gggtcgcgct cgcggcgatg
      121 gaccgtttcg tcgcctgcgc gcgcgcctat atcggtatgc gcctgcgcga cgagcgggcc
      181 ggcttcgatg ctccggaacg ttgcatagtc gtatcgaacc accagagcct gctcgatata
      241 cccgtgctca tgcattactt cagggctgag cgccgccttt tgttcgtggc gaagaaggaa
      301 ctcggttacg gcataccgct gatatcgagc gtgctcagag tcggcggaca cgctctgatc
      361 gaccggggga gcaacccgac cagggcgatg cgcgaactcg gcgctttcgc gaggcggagt
      421 ggagcgcctg ggttctggcc ctcggtcttc ccggaaggca cccgctccaa ggatggctcg
      481 ctcggcaaat tccacactgc cggcctcaga cgcatcgccg acttggctcc ggctcccctg
      541 ctggtcgtgg ccgtggacgg cggctacaag gcgactaagc tgtcatcgtt gctgacgggg
      601 agcgtggggt tcgagtacag ggtcaaggcg ctcggactga tcgagctcga accgggcaag
      661 aagggcgtgc aggaagcgct cgaggccgcc cggtcgctca tcgcgaggca gctggaagcc
      721 tggcgcgccg aagcttaggc gcgccaggcc cgcgtcagcg ccgacgctac ggcctatcga
      781 caataaaaaa accgctcccc aaggggagcg gcctaccggc gccggtttcg cctagctaaa
      841 atcgctcggc ctgcgctcgg tgcgcttgca cttctcgcac tcggcgacgt tcttgacgcg
      901 gccgtcttcg aagagggtct tcatcttgac ttcaccgccg caggggcaat agaagtgatg
      961 cgtggtactc gacgtacgag agttcttact aacctgtccc ataacgaact ccttgggatt
     1021 cgccggcatt gagcggccac ccatccgact gcggccatat gtcgcgtcgg agatagatac
     1081 gggcggcgcc gatcgcgacg ccgattcccg gacgctaaaa tatcgtaaag cgcggtcaag
     1141 tgtcaaccga gcccgtcatc ctataagacc cccggaccct ggtccggatc cgggggcgca
     1201 ccacggaaag gaggaacacc ggccgatcga cgatcgatcg atgccaacct aacaacaacc
     1261 ggtggcggcg gcggttacgg ccgttcgatc cgaggctacg aggaagcgct ggccttctta
     1321 agtagctcct gtagctgttc cttgtggtat aactcgaccg agcggttctc ggcgaattca
     1381 atcgccgagc ggttgaagcc ggagctcgtg acgatcaccc cgcggatgat gcctagcttc
     1441 ttcatattgt cgaggagcga gcggatcgac gagtcgtcga gctgttccga gacgcgcagg
     1501 aagcgtaaca gtcgcggcat cttcttcgtg ccgagccact tatcagactc gttctcgacc
     1561 gtgatgaagt cgacgccgtt cggcgtcggg atcacgtcgc gcacggcgag gttgaggccg
     1621 ttcatcgcgg taagcttgca cagttcgatg aagtcgtcct ggccgcaggt caggtagtcc
     1681 ttgatgcggt cgtcggtgcg gtattcctgg tactgggtca gtttctcggc gacgtcccta
     1741 aattgaggct tcttggcgta gatcttctcc cactgctcga tagccttgtc gatgttcttc
     1801 atcttctcgt agcacagcga caggaagtag cgcccgtaca gggattcgga cgagccgtcg
     1861 tccttggcca gcttgaccgc ccgctcgagc tcgatggcgg cattatcgta ggagccctgg
     1921 ctcatgtagg taccgcctcg ctcgacgagg gccttgatct tgagggccgg gctcttctgc
     1981 gcccgctcga aggcgagcag tgcgccggtg tagtcgttcg attccttcat cagcttgccg
     2041 aggtaatagt agctgtcgta gttggacggg tcgaatttta ggcccagctc gaactccgcc
     2101 ttggcctcga gtgggtgctt ggtcttgtaa aggatgacgc cgagcatgta gtgggccttg
     2161 ccatgacggg gatcgagctc caccgccttg cgtgcgtact tcgtggccac atcggccttg
     2221 ccgcgctcgt cgaagatcct ggaacactgg tagaagtact ccgcgttccg cgggtctccc
     2281 ttggcgagca ggatgtactc cttgagcgcc tcttcgagct ggccgaagcg ctcgtacagg
     2341 tcggctatca gccgtctgaa ctcggtctcg ggaatctcat ggccgaactg gccgatgtcg
     2401 ccgaccgtct tgaactccat cagcgcgagc tccggcttgc cttcggccag gtagatcttg
     2461 cccaggtagt agtgggcggc ggcgttcctc ggttccttgg ctagcatgga cttgacggcc
     2521 ttggtggccg cctgatagcg gccctgcttg atcaattcgg ccaccgccga tagccgcttg
     2581 ggcatgacga tcaggcggat gaggaaaaag ctcaggacgc ctaccgcaat gccgaagaat
     2641 actatgagga tgatgacgaa tgacatcggc ccgccttgct tctctctata ttccggatgg
     2701 cccggtcgcg ctttaaacga agcgcctccc gtgatatcct aataaaaggt atacgatctt
     2761 gtccatctat tctatagtag cgccgatacc ggacgcgcgc aacggcgggg cgtcggctcg
     2821 ctagacgagg gggtgcgatg ctcaggcgag gattctatcg gcgcgcgacg cccggccgct
     2881 tcgtcgcgcg cctggcgttg gctttcgcgc tcggcacggc cgccgtcgga gcctcggagc
     2941 tgtcggatcg cggtatcggc ctgttcatgg agaataaacc gcaggaagcc gcgcccgtgc
     3001 tcgaactggc cgccaaggaa gcggcgactg acgagaaggt gttcctgtac ctcggcatag
     3061 cctaccagca gctcggccgt tgggacgacg ccatcgcggc gttccgtagg gggctcgccg
     3121 cctcggcgct gtaccgtcac cagttcctgt tcaatatagc caactcgttc ttcgcccagg
     3181 gcaagaacgc cttcgccctg gagtactacg accaggcgct ggccctgcgc ggcgattacg
     3241 cccaggccta cctgaaccgc gcgaacgcca agatgcgtct ggccgactac cccggcgcct
     3301 cggcggacta ctcgctgtac ctgagcctcg agcccgggtc gtcccaggcc tccgagatcc
     3361 ggcggctcct cgacctcctc gggtccaagg ccgaggcggc caggacggcg gcggccgagg
     3421 ccgaggccag gcggctcgcc gaggagcagg cgaggaaggc cttgctcgac gaggtggcca
     3481 ggtcgctgct cgaggccgcc gaggcgacga cgaacctgtc cgccggctcg ggcgacgtgc
     3541 aaggctatga atccgacttg tcgctcgacg attgacggcc atcgcccgct ggcgacgccg
     3601 aaacgaaggg aagcatcgtg actgataaag agaagaagcg gttcgtctat atcgggatag
     3661 cggccctgct cgccttggcg gcgatcgcgg ccatcgtcct ggcgctcggg gcgggaggcc
     3721 actccggcga acgggaacgg acgaacatgc tcagcctggc cggcgagtat attgatcggg
     3781 gcgatttcga ccgggcgctc gatatcctgg atcggctgat catcaaggac tcgtccgacg
     3841 ccgaggcgcg atcgctccag gacctggcct tggctcgtaa ggcctcggcc aaggcggccg
     3901 ccgaggaggc ggcgaaggcc ggttccggcg aggcgggcgc tctggcgcag agcctcggcg
     3961 agctaggcaa gtcgctcgag cgcacggcca gcaccgtggc ccagagcgcg acgaggccgt
     4021 ctcagccggc tgccggctcc agtgcgggtt ccgccgacga ttcggccgcg gcacaggcgg
     4081 ccgcgaggaa ggccgaggag gacgcgttgg ccaaggccca gtcggaggcc gaggccgcta
     4141 ggcgcagggc cgccgaagag gagctgtcgc gcaagagcgc cgagctacgc gccaagatgg
     4201 aagcgatcaa cgcgctggtc aaggcggggc gtcagtcgat atcggcagga gactatgcgg
     4261 cggcccgctc ttccttcgac gaggccgtat cgtcgctacc gtcgggcgag gcgaagttca
     4321 gcgcccagac ctacgccgag atagccgagg gctactacga cgggtacaga cgcgacccga
     4381 cgggtgccga gggcctcgag tcggtcaagc aggcccagcg ggtgg
//
LOCUS       DAOPHV010000170         4395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_26931_length_4395_cov_3.914747, whole genome shotgun sequence.
ACCESSION   DAOPHV010000170 DAOPHV010000000
VERSION     DAOPHV010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26931_length_4395_cov_3.914747"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="PLQ29_05760"
     CDS             <1..1379
                     /locus_tag="PLQ29_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPG86189.1"
                     /translation="MDPVSPVLIAAAVVTLAAIVALGVSSGKHVRNASDFDTGGNKAG
                     PVMVAGTLVGTLVGGSATIGTAQLAFMYGLSAWWFTLGCSIGCVALGLWLARRMRASG
                     GGTIQEMISREYGGVARVVTSLLTSVGIVINIIAQILAANALLSTLFGLGPAASALVS
                     VGLMASYVLAGGIRGAGVLGLVKMALIYLTATVCGVLALRLSGGLGAIASALPADRYF
                     SLIARGLAVDLGAGLSVALGVLSTQTYAQAILTARSDGAAKNGALLSAIIVPPVGLLC
                     VFVGYHMRLTHPEMPAAQALPRFIVERLPPALAGVSLAALLLAVVGTGSGMALGFGRI
                     VSNDIYKRYLRPSASGERQLLVSRLVIVGSLAVSALCALGGLGGIILTFGFLSMGLRA
                     AVLLVPMLCALFLPGRMRPCFAMASSVAGLTCMMASWAIGLPFDPLFAGMAASVASAI
                     VGLLVGPR"
     gene            complement(1433..2167)
                     /locus_tag="PLQ29_05765"
     CDS             complement(1433..2167)
                     /locus_tag="PLQ29_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG86190.1"
                     /translation="MRAFALFYKNFMIGAFRNKLAFLVYFAAPIASFVAMFFLLRLTG
                     DESFAAAQAIGLVVYFSMLQATMITSLTIRDREEGAALRIAASPTGGLAYAIGNGAAA
                     FTVLGAQVILFVGFIAFVFPVDLGLGAERLLAILLAFNVASVGLALLLCELSDSSSGA
                     MLLANLVILATSLLGGVFFPAEFMGASARRMAAAFPQYWAMQAIDQARSGAAHAERAL
                     STLILLLFGALFIVGRAALSRRRRSV"
     gene            complement(2164..2901)
                     /locus_tag="PLQ29_05770"
     CDS             complement(2164..2901)
                     /locus_tag="PLQ29_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG86191.1"
                     /translation="MVTLVFARHQVQLFLKSPGVMALFIVGPIFVIFVFGQAFTAIFA
                     AGGQGFDAMQYFGVTLLTMAVFQGSFIASWAVFKERRSNTEVRLGVAPIGRLSATLGA
                     FLGSWLSLAALGCAVMALASVLLSIDYGERLWLSALLICAEALLAASLGVSLAVIFGE
                     ERAANGVMNALVPALVFIGGGYTIIPESGFLHELARFSPLRWINLAMLDLAMPGPGEY
                     IAAALTFSLAASAALMTLACLRLWRKR"
     gene            complement(2911..3852)
                     /locus_tag="PLQ29_05775"
     CDS             complement(2911..3852)
                     /locus_tag="PLQ29_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG86192.1"
                     /translation="MIEIRNASKSFGPTMAVDNVSLTIRDGELFGLLGPNGAGKSTLI
                     RMITGLLAQDGGSVSFFDGPAGTAGPLDAKAVKRMIGLVPQDLAFYQNYSAIENVKFF
                     ASLYGLRGKALTEAAEAALAFAGLSDTGKKPAKAFSGGMKRRLNIACGIAHDPRVVIL
                     DEPTVGIDPQSRNHILRSVEELRRRGRTIIYTTHYMEEAENLCTEIAIMDKGKIIAQG
                     SQERLKLLIKDSSGVTFTLKRPDPVDLGAFKRIRGVTTAKLDELRLCVTSEVGATNLN
                     ELTAEVNRQGLIVTDIETDSPSLETVFLSLTGRSLRD"
     gene            complement(3948..>4395)
                     /locus_tag="PLQ29_05780"
     CDS             complement(3948..>4395)
                     /locus_tag="PLQ29_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPG86193.1"
                     /translation="NGIEAAAEIAVGGTCRVLLLSTFKETELVRGALASGASGYLLKG
                     CSSDEIKEAVRLVHQGHTVFQDEVFEAVRRASANAPGDLSALSERERSLVRLIAEGYS
                     NRQAADSLGLSEGTIKNYVSSILDKLGLQQRTQIAIYCVTGRRDFS"
BASE COUNT          704 a         1404 c         1509 g          778 t
ORIGIN      
        1 ccatggatcc agtatcaccg gttctgatag ccgcggcggt cgtcacgctc gcggccatcg
       61 tcgccctggg cgtatcgagc ggcaagcacg tgaggaacgc ctcggatttc gataccggcg
      121 ggaataaggc cgggccggtc atggtcgccg gtacgttggt cggtacgctg gtcggcgggt
      181 cggcgacgat aggcacggct cagttggcct tcatgtacgg cttgtcggct tggtggttca
      241 cgctcggttg ctcgataggc tgcgtcgcgc tcgggctctg gctggcgcgg cgcatgcggg
      301 cctcgggcgg cggcaccatc caggagatga tcagccgaga gtacggcggc gtcgcgaggg
      361 tcgtcacttc gctcctgacc tctgtcggca tcgtcatcaa catcatcgcg cagatcctgg
      421 ccgccaatgc gctcctttcg acactgttcg ggctcgggcc ggcggccagc gccctcgtca
      481 gcgtcggcct gatggcctcg tacgtgctgg ccggcggcat ccgaggggcc ggcgtcctgg
      541 gcctggtcaa gatggcgctg atctacctga cggcgacggt atgcggcgtc ctggccctcc
      601 ggttgtcggg cggcctgggc gcgatagcct cggcgctgcc ggcggatcgc tacttcagcc
      661 tgatcgcccg tggcctcgca gtcgatctgg gcgccggcct atccgtggcg ctcggggtgc
      721 tatcgaccca gacctacgcc caggccatcc tgacggccag gagcgacggg gcggcgaaga
      781 acggcgcgct gctgtcggcg atcatcgtgc ccccggtcgg gctcctgtgc gttttcgtag
      841 gctaccacat gcgcctgact cacccggaga tgccggcggc ccaggccttg cctcgcttca
      901 tcgtggagcg gctgccgccg gccctggccg gcgtcagcct ggccgcgctg ctactggcgg
      961 tcgtcggtac cggctccggc atggcgctcg gcttcgggcg tatcgtctcc aacgacatct
     1021 ataagcgcta cctccgcccg agcgcgagcg gcgagcgcca gctcctggtc tcccgtctgg
     1081 tgatagtcgg ctccttagcg gtctcggcgc tctgcgctct cggcggtctc ggagggatca
     1141 tcctgacctt cggtttcctg tcgatgggcc tgcgcgcggc tgtcctgctc gtgccgatgc
     1201 tgtgcgccct gttccttccc gggcgcatgc gcccgtgctt cgcgatggcc tcgagcgtgg
     1261 ctggcctgac ctgcatgatg gcgtcctggg cgattggact gccgttcgac ccgctgttcg
     1321 ccggcatggc ggcctcggtc gcgtcggcca tcgtcggcct gctcgtaggc ccaagatgat
     1381 ggtcggccga gcgaggcagg cctcgccccc ggtaaggcgg agcgtccgac gttcagaccg
     1441 atcgcctccg cctggatagc gcggctcggc cgactatgaa cagcgccccg aataggagca
     1501 gtatcagcgt cgacagggcc cgttcggcgt gtgcggcgcc ggagcgggcc tggtctatcg
     1561 cttgcatcgc ccagtactgc gggaaggccg ccgccatccg gcgagccgac gcgcccatga
     1621 actcggccgg gaagaagacg ccgcctaaga ggctcgtggc caggatgacg aggttggcca
     1681 gcagcatcgc ccccgagctc gagtcggaca gctcgcagag gagaagggcc agcccgaccg
     1741 aggccacgtt gaaggcgagt aggatggcca agagccgctc ggccccgagg cccagatcga
     1801 ccggaaatac gaatgcgatg aagccgacga acaggatgac ctgggcgccg agcaccgtga
     1861 aggccgcggc gccattcccg atggcatagg cgaggccacc cgtcggcgag gccgcgatgc
     1921 gtagggctgc gccctcttcc ctgtccctga tggtcaggct ggttatcatc gtggcctgga
     1981 gcatcgagaa atagacgacg aggccgatgg cctgggcggc ggcgaacgac tcgtcgccgg
     2041 tgaggcgaag caggaagaac atcgccacga agctggcgat cggagcggcg aagtagacga
     2101 ggaaggcgag tttgtttctg aatgccccga tcatgaagtt tttatagaac agcgcgaagg
     2161 ctctcatcgt ttcctccata gcctaaggca ggcgagcgtc atcagcgcgg cggaagccgc
     2221 caggctgaag gtcagggcgg cggcgatgta ctcgccggga cccggcatgg ctaggtcgag
     2281 catggccagg ttgatccagc gtagtggcga gaagcgcgcc agctcgtgca ggaagccgct
     2341 ctccggtatg atcgtatagc cgcctccgat gaagacgagc gccggcacga gcgcgttcat
     2401 cacgccgttg gccgccctct cctcgccgaa gatgaccgcc agcgagacgc cgaggctcgc
     2461 cgccagcaag gcctcggcac agatgagcag cgccgaaagc cagagccgct cgccgtagtc
     2521 gatcgacagg agtaccgagg cgagcgccat gactgcgcag ccgagcgccg cgagcgacag
     2581 ccacgagccg aggaaggcgc cgagcgtcgc cgacaggcgg ccgatgggcg ccacgccgag
     2641 ccgaacctcg gtgttcgacc gcctttcctt gaagacggcc cacgaggcta tgaaggagcc
     2701 ctggaacacg gccatcgtga gcagggtgac gccgaagtac tgcatggcat cgaagccctg
     2761 gcccccggcg gcgaagatgg cggtgaaggc ctggccgaag acgaagataa cgaagatcgg
     2821 gccgacgatg aacagcgcca tcactcctgg gcttttaagg aacagctgta cctggtgcct
     2881 ggcgaatact agggttacca tgtcgtccac ctagtcccgc agggaacggc cggtaaggct
     2941 caggaatacc gtctcgagcg atggcgagtc ggtctcaata tcggtgacga tgagtccctg
     3001 ccggttgacc tcggctgtca gctcgttcag gttggtcgcg ccgacctcgc tggtcacgca
     3061 gaggcgcagc tcgtcgagct tggccgtcgt gacgccgcgg atgcgcttga acgcgccgag
     3121 gtccaccggg tcgggccgct tgagggtgaa ggtgacgccg ctcgagtcct tgatcagcag
     3181 cttgagccgt tcctggctgc cctgggctat gattttgccc ttgtccatga tggctatctc
     3241 ggtacagagg ttctccgctt cttccatata atgcgtcgta tagatgatgg tacggccgcg
     3301 gcgcctgagc tcctcgacgg agcgcagaat atggttgcgc gactgggggt cgataccgac
     3361 cgtcggctcg tccaggatga cgacgcgcgg gtcgtgggct atgccgcagg cgatattcaa
     3421 cctgcgcttc atgccgccgg agaatgcttt ggccggtttc ttgcccgtgt cggagaggcc
     3481 agcgaacgcc agcgccgcct cggcggcctc ggtcagggcc ttgccgcgga ggccgtacag
     3541 gctggcgaag aacttgacgt tctcgatcgc gctatagttc tggtagaagg ccaggtcctg
     3601 gggtacgagc cctatcatgc gcttgacggc cttcgcgtcg agcgggccgg ccgttcccgc
     3661 cgggccgtcg aagaacgata ccgagccgcc gtcctgtgcc aggaggccgg tgatcatgcg
     3721 gatgagggtc gacttgccgg cgccgttggg gccgagcagt ccgaatagct cgccatcgcg
     3781 tatggtcagc gagacgttgt ccacggccat ggtaggccca aagctcttgc tcgcgttccg
     3841 aatctctatc atctcgtcct cctcgcccta tcgtggttcg agcatagcga cggcggagcg
     3901 cggcgggtag tacgtttggt aacgcgccgg agtgactaaa gtcaggccta ggagaagtcc
     3961 ctgcggccgg tcacgcagta gatggctatc tgggtgcgct gctggagccc gagcttgtcc
     4021 aggatggacg agacgtagtt cttgatcgta ccctcggaca ggccgaggct gtcggccgcc
     4081 tgccggttcg aatagccctc cgctatcagc ctgacgaggg agcgctcccg ctcgctcaag
     4141 gcggataggt cgcctggcgc gttggccgag gcccgcctga cggcctcgaa cacttcgtcc
     4201 tggaagaccg tgtgcccctg atggacgagc ctgaccgctt ccttgatctc gtcgctcgag
     4261 cagcctttga gtagatagcc cgacgcgccg ctcgccagtg cgccgcgcac gagctcggtc
     4321 tccttgaagg tggacaggag taggaccctg cacgtgccac cgaccgcgat ctcggccgcc
     4381 gcttcaatgc cgttc
//
LOCUS       DAOPHV010000171         4391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_26965_length_4391_cov_3.773524, whole genome shotgun sequence.
ACCESSION   DAOPHV010000171 DAOPHV010000000
VERSION     DAOPHV010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26965_length_4391_cov_3.773524"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1866
                     /locus_tag="PLQ29_05785"
     CDS             214..1866
                     /locus_tag="PLQ29_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HPG86194.1"
                     /translation="MNVVVVLIELVGSLGLFLYGMRILSDGIQRAAGDRLKRALNLMT
                     GTTFKALLTGAGLTALIQSSSVTTVMVVSFVNAGLLTVAQAAGVIFGANIGTTITAWI
                     VSLIGFDFHISALALPMVGAGFIMMTLPKRKSRLRDYGEAALGFGLLFMGLDFLAHAI
                     PRPSADVIRFLEGLSGMGVLSILIAVATGTVLTILIHSSSASTAIVITLAVEGVIGFE
                     MAAGLVLGCNIGTTIDAFLASIGAKANARRAAWLHILFNVLGSLWAVILFKPFLALVN
                     LMAGGSTVAQHIAMMHTVFNVINAAVFAPFAKQLSELVTRFVKAKPGEDDLPQKLEYV
                     AAPLVDSPELNLIRAQKEISDMAGLCGSMFVRWRELLRDGTEDVSDEVERFRGLEDYA
                     DQMRDELSRFLLECAGHEVSADRQRDIGKMMRIVVDLEDITDDCFSLVLLLDKARSRG
                     LVVDAEEIASLGPYTLLVENFLRFVKDNINGAISAEQLAIAQDLENSIDDFRTTLKKK
                     ARKRLKSGENVKAELLFMDMVRHIEKIGDHAFGVAHALRTRS"
     gene            1954..2928
                     /locus_tag="PLQ29_05790"
     CDS             1954..2928
                     /locus_tag="PLQ29_05790"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPG86195.1"
                     /translation="MKTHRATTKWLILAVMGALAIAWLIIGLWPRSGMAAPPLWRAHA
                     DGAPLVLAHQGGAMERPSSSDLAFERASEIGVDAFELDVVLTADGVLAVIHDTTLDRT
                     TDGVGPVGDRTYAEIRFLDAGYGLLGEDGEVVRDPARNPYIGIGAYVPSLEELFREYP
                     SMPMLIEIKDLGDRGLASARELRRLIDAYGRATTTMVACNDEEPLLEFDSLPGERVAR
                     AASKPEAYRFYARHALGLNALNNRAPHEAMSLSLTSGLGPFTIDLTAERLRRDCVARG
                     LSTMYWTVNDVATMERLLSMATSTGGIDGIITDRPSELLAALSRRGIR"
     gene            complement(2937..3602)
                     /locus_tag="PLQ29_05795"
     CDS             complement(2937..3602)
                     /locus_tag="PLQ29_05795"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPG86196.1"
                     /translation="MRLYRVPERIDAFLFDIDGTLYTDREYGRFQIEVLVRELARIRG
                     EPYDEVAALVERTRAALADAEGRASTSLGNAMAALGVDIGTGVEWRRRLIRPERFLSV
                     DPALVAALSGLRDRYGSALVAVTNNPRSVGEATLEALGVHGLFSAVVGLDDTMRSKPA
                     AEPYLLAARLAGAEPGACISVGDRYDVDLAVPLELGMGAALVDGVEDVYGLAEAIVGG
                     AGT"
     gene            complement(3605..>4391)
                     /gene="prs"
                     /locus_tag="PLQ29_05800"
     CDS             complement(3605..>4391)
                     /gene="prs"
                     /locus_tag="PLQ29_05800"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HPG86197.1"
                     /translation="AYPYSRQHKKKGREGLTAARLGQIFENYGVQRIITLDIHSREIE
                     NCFNKLRLENLHASYQVIEKLAGIVDVSDENLVVLSPDTGAVDRNKFYASTLQKPLAM
                     LYKERDYSKVTKDATDSNITEMKLLGDIHGKIVFMADDMLGTGGTLLKAMRYLKDQGA
                     TKVICAVSLPLFSANAIDDFDKAYREGLFYRIIGTNAVHHGQLLKKEWYIEADVTTLF
                     AQIISLLHHNRSLSPMLDNREIIAKKIKKAMAAQAAHQPTLPL"
BASE COUNT          762 a         1398 c         1454 g          777 t
ORIGIN      
        1 gatcacggaa gcgagcgccc ggaaggcacg gcgcggcgac cgagacgata ggggattcat
       61 gcacttacta tagcgcgaat ccagccagac cgtatacgcg accagcgcgc tttattttta
      121 ggctcgatta cgctataacg gagcgtacgc gacgctcacc cccacctcgt cgcgcggctg
      181 tcgggccgcg tcgatgcctc gaaaggctac ttaatgaatg ttgtcgttgt actgatagaa
      241 ctggtcggat cgctcggatt attcctctac ggcatgcgca tactgtccga cggcatccag
      301 cgagccgccg gcgaccggct caagcgcgcc ctcaacctga tgaccggcac caccttcaag
      361 gccctcttga ccggagccgg gctgaccgca ttgatccaat cgtcgtcggt gacgacggtc
      421 atggtcgtct ccttcgtcaa cgccgggctt ctgaccgtcg cccaggccgc cggcgtcatc
      481 ttcggcgcga acatcggaac gacgatcacg gcctggatcg tgtcgctgat cggcttcgac
      541 ttccatatct cggcgctcgc cctgccgatg gtcggcgccg gcttcatcat gatgaccctg
      601 ccgaaacgca agtccagact acgcgactac ggcgaggcgg cgctcggttt cgggctcctg
      661 ttcatggggc tggacttctt ggcgcacgcg atcccgaggc cctcggccga cgtgatccgc
      721 ttcctcgagg gattgtccgg catgggcgtg ctgtcgatcc tgatagccgt ggcgaccggc
      781 acggtactga ccattctgat acactcatcg agcgcctcga cggccatcgt gatcacgctc
      841 gccgtggagg gtgtcatcgg cttcgagatg gccgcggggc tggtcctcgg atgcaacatc
      901 ggcaccacga tcgacgcgtt cctcgcctcg atcggggcca aggcgaacgc gagacgcgcg
      961 gcctggttgc acatcctgtt caacgtgctc ggatcgctct gggccgtgat cctattcaag
     1021 ccattcctag ccctcgtcaa cctgatggcc gggggctcca ccgtggccca gcacattgcg
     1081 atgatgcata cggtcttcaa cgtgatcaac gcggcggtct tcgcgccctt cgccaagcaa
     1141 ctgtccgaac tggtaaccag gttcgtcaag gccaagcccg gcgaggacga cctgccgcag
     1201 aaactcgagt acgtggccgc cccgctcgtc gatagcccgg agctcaacct gatccgggcg
     1261 cagaaggaga tctcggacat ggccggccta tgcggctcga tgttcgtccg ttggcgcgag
     1321 ctgctgcgcg acgggaccga ggatgtctcc gacgaggtgg agcgcttccg cggcctggag
     1381 gactacgccg accagatgcg cgacgagctg tcgagattcc tactcgagtg cgccggacac
     1441 gaggtgagcg ccgacagaca gcgcgacatt ggcaagatga tgcgcatcgt cgtcgacctg
     1501 gaggacatca ccgatgactg tttcagcctc gtgctgttgc tggacaaggc ccgctcgcgc
     1561 gggctcgtcg tcgacgcgga ggagatagcg agcctggggc cctatactct gctcgtcgag
     1621 aacttcctgc gcttcgtcaa ggacaacatc aacggcgcta tcagcgcgga gcagctcgcc
     1681 atagcccagg acctggagaa cagtatcgac gacttccgta cgacgctcaa gaagaaggcg
     1741 cgcaagcggc tcaaatccgg cgagaacgtg aaggccgagc tcctattcat ggacatggtc
     1801 aggcacatag agaagatagg cgaccatgcg ttcggcgtgg cccacgccct gaggaccagg
     1861 agctaggcga gcgacgatcg catcgagagc gacgatcgca tcgcgagcga cgatcgcgct
     1921 tgcgggttgc cggttcggtc gtatactgtc cgcatgaaaa cgcatagagc tacgacgaaa
     1981 tggctcatcc tcgcggtgat gggcgcgctc gccatcgcgt ggctgatcat cggcctgtgg
     2041 cccaggtccg gcatggccgc gccaccactc tggcgggccc acgccgacgg cgctccgctc
     2101 gtgctggcgc atcagggcgg agcgatggag cggccgtcgt cgagcgacct ggccttcgag
     2161 cgcgcctccg agattggcgt cgacgcgttc gagctcgatg tggtactcac ggccgacggc
     2221 gtcctggcgg ttatccacga tacgaccctc gaccgtacga ccgacggcgt cgggccggtc
     2281 ggtgacagaa cctacgccga gatacgcttc ctcgacgccg gctacggcct cctgggtgag
     2341 gacggcgagg tcgtgcgcga ccctgccagg aacccctaca taggcatcgg ggcctacgtg
     2401 ccgtcgctcg aggagctgtt ccgcgagtac ccctccatgc ccatgctgat agaaataaag
     2461 gacttaggcg accggggctt ggcctctgcg agggagctca ggcggctgat agacgcctac
     2521 gggcgcgcga ccaccacgat ggtcgcctgc aacgacgagg aacccctcct ggagttcgac
     2581 tcgctacccg gtgaacgggt cgctcgggcc gcgtccaagc ccgaggcgta ccgcttctac
     2641 gcccggcacg cgctcgggct gaacgcgctc aacaaccggg ctccgcacga ggcgatgagc
     2701 ctgtcgctga cgagcgggct cggcccattc acgatcgacc tgacggccga gcgcctgcgc
     2761 cgggactgcg tagccagggg actctcgacg atgtactgga cggtgaacga cgtggcgacg
     2821 atggaacgcc tactatcgat ggccacgtcg acgggcggga tcgacggcat catcaccgac
     2881 cggccgagcg agctcctggc cgccctcagc cggcgcggca tcaggtagcg tcgctatcat
     2941 gtaccggcgc cgccgacgat agcctcggcc aggccgtaga cgtcctcgac gccgtcgacg
     3001 agcgcggcgc ccatgcccag ctcgagcggt acggcgaggt cgacgtcgta gcggtcgccg
     3061 accgaaatgc aggcgccagg ctcggcgccg gcaagtcggg cggctagcag gtagggctcg
     3121 gccgccggct tggagcgcat ggtatcgtcg aggccgacga cagccgagaa taggccgtgg
     3181 acgccgaggg cctcgagcgt cgcctctcct accgacctgg ggttgttggt gacggcgacg
     3241 agcgccgagc cgtagcggtc gcggagcccg gagagggcgg cgacgagcgc agggtcgact
     3301 gacaggaagc gttcggggcg tatcaggcgc cgccgccact cgacgccagt ccctatgtcg
     3361 accccgaggg cggccatcgc gttgccgagg ctggtggagg cgcggccttc ggcgtcggcc
     3421 agagcggccc gcgtacgctc gaccagggcc gcgacctcgt cgtatggctc gccccggatg
     3481 cgggccagct cacgcaccag gacctcgatc tgaaagcggc cgtactcgcg atccgtgtac
     3541 agcgtgccgt cgatatcgaa gaggaacgcg tcgatacgct cgggaacgcg atagagcctc
     3601 aaagtcagag cgggagcgtc ggctggtggg cggcctgcgc ggccatcgcc ttcttgatct
     3661 tcttggcgat gatctcgcgg ttgtcgagca tcggcgacag gctccggttg tggtgcagca
     3721 gcgatatgat ctgcgcgaac agcgtggtca cgtcggcctc gatgtaccac tccttcttga
     3781 ggagctggcc gtggtgcacg gcgttcgttc cgattatccg gtagaacaga ccctcgcggt
     3841 aggccttgtc gaagtcgtcg atcgcgtttg ccgagaacag cggtagcgat acggcgcaga
     3901 tcaccttcgt ggcgccctgg tccttgaggt agcgcatagc cttgagcagc gtgccaccgg
     3961 tgccgagcat atcgtcggcc atgaacacga tcttgccgtg gatgtcgccg agcagcttca
     4021 tctcggtgat gttggagtcg gtcgcgtcct tggtgacctt ggagtagtcg cgctccttgt
     4081 acagcatggc gagcggcttc tgcagcgtgc tcgcgtaaaa tttgttgcgg tcgaccgcgc
     4141 cggtgtccgg ggagagcacg accaggttct cgtcgctgac atcgacgatg ccggccagct
     4201 tctcgatgac ctgataggaa gcgtgcaggt tttcgaggcg cagcttgttg aagcagttct
     4261 ctatctcgcg ggagtggatg tcgagggtga tgatgcgctg gacgccgtag ttctcgaaga
     4321 tctgcccgag ccgcgcggcg gtcaagccct cgcggccctt cttcttgtgc tgcctggagt
     4381 acggataggc g
//
LOCUS       DAOPHV010000172         4377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_27096_length_4377_cov_4.769320, whole genome shotgun sequence.
ACCESSION   DAOPHV010000172 DAOPHV010000000
VERSION     DAOPHV010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27096_length_4377_cov_4.769320"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLQ29_05805"
     CDS             complement(<1..396)
                     /locus_tag="PLQ29_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPG86198.1"
                     /translation="MKKYLVLAGNIGAGKSTLVGLLAERLGYRPYFEPVAENPYLEDF
                     YADMERWAFHSQAFFLTHRVRSHRALMDDAYSVVQDRSMYEDAEVFARNLYERGSMSA
                     RDWKSYSELYRTLTSLLPPPDLVVYLRASV"
     gene            526..2355
                     /gene="typA"
                     /locus_tag="PLQ29_05810"
     CDS             526..2355
                     /gene="typA"
                     /locus_tag="PLQ29_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPG86199.1"
                     /translation="MTHERSINSSIRNIAIIAHVDHGKTTLVDAMFKQSGLFREGQQT
                     QERLMDSMDLERERGITIAAKNCSIVWKGVKVNILDTPGHADFGGEVERALSMVDGAI
                     LLVDASEGPLPQTRFVLSKALAGGLAVIVVINKIDRQDARPAEVLSEIYDLFIDLGAN
                     DAQLDFPVLYAIGRSGIAKDSLDGPGDNLHPLLDAILDDLPAPSYDAAEPFQMLVSDL
                     AYSDYFGRQAIGKVINGRAHSKDGLVRIGEGDKREALSVSKLHTYSGVKLVEAEYADT
                     GDIIVLSGIENVHIGDTICTRDAPKALPRLVVDEPTVAMRFSVNTSPFSGMDGKKVQS
                     LKLFERLRKETLNNVSIQVQEAGGGVFIVRGRGEFQLVILIEMLRREGFEICVGRPEV
                     IFKYVDGEKLEPIEHLVVDCAEDFAGVVTDKLSARKGRMLSYTCHDGGRVHLEFSVPS
                     RALIGYRDAFLTDTKGTGMMNSYLSGHEQYRGDIVNRFTGSLVSDRSGAAVTYGLYHL
                     EPRGHLFIVPGEQVYEGMIVGEHNLDSELDVNPCKEKKLSNMRSVLKDEALTLTPIQP
                     MTLERAIQFLADDEMVEVTPNFLRLRKTILAYQDRKCSKRDAS"
     gene            complement(2362..2916)
                     /locus_tag="PLQ29_05815"
     CDS             complement(2362..2916)
                     /locus_tag="PLQ29_05815"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HPG86200.1"
                     /translation="MSEFSSYCAYCAQAAPDDPNRSYHDLEYGFPILDDAGLFGRLVL
                     EINQAGLSWVTILRKKDAFMRAYDGFDVDTVAAYGEADVARLLADPGIVRNRRKVAAA
                     IENARRIVALRPGFGSFRGWLDVYHPRTKEAWVKLFKRSFTFVGGEIVGEFLMSTGYL
                     PGAHVAGCPIGQLAIAAGAPWAVR"
     gene            complement(2929..3960)
                     /locus_tag="PLQ29_05820"
     CDS             complement(2929..3960)
                     /locus_tag="PLQ29_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86201.1"
                     /translation="MLRGYSIKAAMALSARALLTLVACAALALTACAGSPREAPVDPE
                     RTVAAVDRPDPRSVLAGAITSSLYGELDSARRAVDEALALAMDRTTLALGLEARAWIL
                     LLSGSDEEARRALDESAAVGAPSSPGRAVLEFRLAATKDLAAAKERAALAVAQGIGER
                     AERAIAVMLGEADLRDLPIDTPEAAAEYATYLAGYGMSPAAALPAATGSTEPPVVVVT
                     EPESHGADDTTVQVARLACRDALSRRPSLRVVDADSRRAALDELELSLAGSPAVRRDL
                     ALGELFSADYVASGSVVAADSGWLIAYSLSSAKDGHIVASDFSMAADHRAIMAAASRF
                     AASVDGLAP"
     gene            complement(4013..>4377)
                     /locus_tag="PLQ29_05825"
     CDS             complement(4013..>4377)
                     /locus_tag="PLQ29_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86202.1"
                     /translation="QTSEFFSRLSEEIQAMSSTFGDLLMKLQEVSAGTAGVTEAIRGF
                     SELSVAAGAAVDETREAFRSTVARAASSREVASAMRQAMDRMAASCDELLAQAASLSE
                     LGQENVRKMEGLRDKLGA"
BASE COUNT          781 a         1419 c         1458 g          719 t
ORIGIN      
        1 caccgaggcg cgcaggtaga cgaccaggtc agggggcggc aggaggctcg tcagcgtgcg
       61 gtacagctcg ctgtagctct tccagtcgcg cgcgctcatc gagccgcgct cgtacaggtt
      121 gcgggcgaag acctccgcgt cctcatacat ggagcggtcc tgtaccaccg agtaggcgtc
      181 gtccatgagg gcgcggtggg agcgtacgcg atgggtcagg aagaaggcct gggagtgaaa
      241 ggcccagcgc tccatgtcgg cgtagaagtc ctccaggtac ggattctccg ccaccggctc
      301 gaaatagggc cggtagccga gtcgctccgc gaggagccca acgagagtgg attttcctgc
      361 gcctatgttg ccggcaagaa cgagatattt cttcatatac tgttgtctcg ggcgccagtc
      421 taggctcgcc cggctttttc cgcaagtccg ggacgtatac caacggtgcc actttcaggt
      481 atactgggtt atctcccgcc gtaaaaagca catcaggact accttttgac ccatgaacgc
      541 tcgattaact cctcgatacg caatatcgcc ataatagcgc acgtagacca cggcaagacg
      601 acgctcgtag acgccatgtt caagcaatcg ggcctgttcc gtgaagggca gcagactcag
      661 gagcgcctga tggacagcat ggatctggag cgcgagcgcg gtatcacgat agccgccaag
      721 aactgttcca tcgtctggaa gggcgttaag gtcaacatcc tcgatacgcc tggtcacgcc
      781 gacttcggcg gcgaggtgga gagggcgctg tccatggtcg acggcgctat actgctcgtc
      841 gacgcgtcgg aaggccccct gccgcagacc cgattcgtgc tgagcaaggc gctggccggt
      901 ggcctggcgg tcatcgtcgt cataaacaag atcgacagac aggacgcccg cccggcggag
      961 gtcctaagcg agatatacga cctgttcata gatctgggcg ccaacgacgc ccagctggat
     1021 ttccccgtgc tgtacgctat cggccgctcc ggcatagcca aggattcgct cgacggaccc
     1081 ggcgacaacc tacacccgct cctcgacgcc attctcgacg acctaccggc gccgtcctac
     1141 gacgcggccg agccgttcca aatgctcgta tcggacctgg cgtactcgga ctatttcggc
     1201 cgccaggcta taggcaaggt aatcaacggc cgggcgcact ccaaggacgg cctcgtccgc
     1261 atcggcgagg gcgacaagcg cgaagccctg tcggtgtcga agctccatac ctactcgggc
     1321 gtcaagctcg tagaggcgga gtacgccgat accggtgata tcatcgtgct gtcaggcatc
     1381 gagaacgtgc atatcggcga caccatctgc acccgcgacg cgcccaaggc cctgccgcgg
     1441 ctggtcgtcg acgagccgac cgtcgccatg cgcttctccg tcaacacctc gccattctcc
     1501 ggcatggacg gcaagaaggt ccagtcgctc aagctgttcg agcgcctgcg caaggagacg
     1561 ctcaacaacg tgtccatcca ggtgcaggag gcgggcggcg gtgtattcat agtacgcggc
     1621 cgcggcgaat tccagctggt catcctgata gagatgctca ggcgcgaggg cttcgagata
     1681 tgcgtcggcc gacccgaggt catattcaag tacgtggacg gagagaagct ggaaccgata
     1741 gaacacctcg tcgtggactg cgccgaggat ttcgccggcg tcgtcaccga caagctgtcg
     1801 gcgcgcaagg ggcgcatgct cagctatacc tgccacgacg gcgggcgcgt ccacctggaa
     1861 ttctccgtgc cgtcgcgggc gctgataggc taccgcgacg ccttcctgac cgataccaag
     1921 ggtaccggca tgatgaactc gtacctgtcc ggtcatgagc agtaccgcgg cgacatcgtc
     1981 aaccgcttca ctggttctct cgtgtcggac cgatcgggcg cggcggtcac ctacggcctg
     2041 taccacctgg aaccgcgcgg gcacctgttc atcgtgcccg gggagcaggt ctacgagggc
     2101 atgatcgtgg gcgagcacaa cctcgactcc gagctggacg tcaatccgtg caaggagaag
     2161 aagctgtcca acatgcgctc ggtgctcaag gacgaggcgc tgacgctgac gccgatacag
     2221 ccgatgacgc tggagcgcgc catacagttt ctcgccgacg acgagatggt agaggtgacg
     2281 cccaatttcc tacggctgcg taagacgatc ctggcctacc aggaccgcaa gtgcagcaag
     2341 cgggacgcct cgtaatcgag gctagcgcac ggcccacgga gcgccggcgg ctatcgccag
     2401 ctgcccgatg gggcagccgg cgacgtgggc cccgggcagg taacccgtgc tcatcaggaa
     2461 ctcgccgacg atctcgccgc cgacgaacgt gaagctgcgc ttgaacagct tgacccacgc
     2521 ttccttggtt cgcgggtggt agacgtccag ccagcccctg aacgagccga agccggggcg
     2581 tagcgcgacg atgcggcggg cgttctctat cgcggccgcc accttgcggc ggttgcgtac
     2641 gatgccaggg tccgcgagca ggcgcgccac gtcggcctcg ccgtacgcag cgaccgtatc
     2701 gacgtcgaag ccgtcgtagg cgcgcatgaa cgcatctttc ttcctgagta tggtgaccca
     2761 ggacaggccg gcctggttga tttcgaggac gagcctaccg aagaggccgg cgtcgtccag
     2821 gatggggaag ccgtattcga ggtcgtggta ggagcggttc gggtcgtccg gggcggcctg
     2881 ggcgcagtac gcgcagtagc ttgaaaactc ggacacgcgt cgtacccgtt acggagccag
     2941 gccgtcgacc gaggcggcga agcgggacgc ggccgccatg atcgcccggt ggtcagcggc
     3001 catggagaaa tcgctggcga cgatatgccc gtccttggcc gaggataggg agtacgcgat
     3061 gagccagcct gaatcggcgg ccacgacgct acccgacgcg acgtagtcgg ccgagaagag
     3121 ctcgcctagg gccaggtctc gccgcaccgc cggcgagccg gcgagcgata gctccagctc
     3181 gtcgagggcc gcgcgcctcg agtccgcgtc gacgacgcgt aggctggggc gtctggataa
     3241 cgcgtcgcgg caggccaggc gcgccacctg gacggtcgtg tcgtcggcgc cgtgcgactc
     3301 gggctcggtg acgacgacga ctggcggctc ggtcgagccg gtggcggccg gaagcgcggc
     3361 cgccggactc atgccgtagc cggcgaggta cgtcgcgtac tcggcggccg cctcgggcgt
     3421 gtcgatgggc agatcacgaa ggtcggcctc gccgagcatc accgcgatag cgcgctcggc
     3481 gcgctcgcct atgccctggg ccacggcgag cgcggcgcgc tccttggcgg cggccagatc
     3541 cttggtcgcg gcgagcctaa actcgagcac ggctcgtccg gggctcgagg gggcgcccac
     3601 ggcggccgac tcgtcgagcg cgcgtcgcgc ttcctcgtcc gagccggaca ggagcagtat
     3661 ccaggccctc gcctcgagcc cgagcgccag tgtcgtcctg tccatggcca gcgcgagcgc
     3721 ctcgtccacg gcgcgcctcg cgctatcgag ctcgccgtac agcgacgagg tgatggcgcc
     3781 ggcgagaacg gagcgcgggt cggggcgatc gaccgcggct acggtccgct ccggatcgac
     3841 gggcgcctcg cggggagatc ccgcgcaggc ggtaagcgcc agcgcggcgc aggcgactag
     3901 ggtgagtaac gcgcgggccg atagggccat cgcggctttt atgctgtagc ctcgtagcat
     3961 cgttcctccg ggtttcacgg ctcgaagcgg ccgctcatcg gcctttcggg ccctaggccc
     4021 ccaacttgtc gcggaggcct tccatcttgc gcacgttctc ctgaccgagc tcgctcagcg
     4081 aggcggcctg ggccagtagc tcgtcgcagg aggcggccat cctatccatt gcctgacgca
     4141 tcgccgaggc gacctcgcgg gacgaggcgg cgcgggccac ggtcgacctg aacgcctccc
     4201 tggtctcgtc tactgcggcg ccagcggcta cagatagctc cgaaaagccc ctgatcgcct
     4261 cggtaacccc ggccgtgcca gccgatactt cctggagctt catcagaagg tctccgaagg
     4321 tcgacgacat cgcctgtatc tcctcggaca gcctggagaa gaactcgctg gtctggg
//
LOCUS       DAOPHV010000173         4371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_27160_length_4371_cov_3.223123, whole genome shotgun sequence.
ACCESSION   DAOPHV010000173 DAOPHV010000000
VERSION     DAOPHV010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27160_length_4371_cov_3.223123"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PLQ29_05830"
     CDS             complement(<1..543)
                     /locus_tag="PLQ29_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86203.1"
                     /translation="MRRLGPTLAATLSSAVALALAVSCSSSPPEIVSLAVRLIVVTTD
                     PGGARDERLSVFASVADAQGVGDVEYLYVVHDGDELCWTMGPSEWRRSDDGSSVWLGA
                     NSLDAPGTTIPRGTYRLILIDKAGERAERSFSLSAPETSAYDTPSLRLSGTTVVVATP
                     YPTNTAFFLDAGGNVTHTAAI"
     gene            complement(548..1846)
                     /gene="hisS"
                     /locus_tag="PLQ29_05835"
     CDS             complement(548..1846)
                     /gene="hisS"
                     /locus_tag="PLQ29_05835"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPG86204.1"
                     /translation="MAEIIQPRVLKGFRDSLPRAEIERAAMIEALTGAFRSFGFVPID
                     TPVLEYAEILLGKAGGETEKQVYRFADNGGRDVAMRFDLTVPFARFMAEHSGDLYLPF
                     RRYHMAKVWRGENTQRGRYREFMQCDFDIVGVDTASADFDIVATIARSMTELGVPGAT
                     IRVNHRGIFNRFLERAGVADRSVEALRVVDKLAKIGEPETRRQLTELVGVAAADEIMA
                     FIAGGEAFGPTLDRMTRLAGGPNPDSDRLSEIFSMAEACGLSSSLELCPSITRGLDYY
                     TGVVFETFLRELPGIGSVCSGGRYNDLASLYTKQRLPGVGASVGIDRLIAALEELGRS
                     GAKAGYTRVLVANVEPALTPEYQAAAAVLRDAGIACEVYPEPRKLALQYAYAEQKGAS
                     YTLIRGSDDKARGLWLLRDLAARSNAEHADLGSVIAALGD"
     gene            1980..2741
                     /locus_tag="PLQ29_05840"
     CDS             1980..2741
                     /locus_tag="PLQ29_05840"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPG86205.1"
                     /translation="MLFVIDAGNTNIVGGVYDSGRLVDTLRMRTVQGKTEDEYGVIVR
                     AVLLDRGIDPSTISRVIVSSVVPQLTAAMTKMSRHLFGVEPVLVGPAVYARLPVTVVA
                     ANEIGSDLVADAVAAWNRFGGACLVVDFGTALTFTAIDAKAAIIGVSIAPGLGTAAGS
                     LSSATAQLPLVPLEAPASAMGRNTVEAIQAGVVLGYTGLVEYMVGRIKGELGGAKVVA
                     TGGLCRVVSELTEVFDAIDPDLTLNGLAEMERYLF"
     gene            complement(2746..3189)
                     /locus_tag="PLQ29_05845"
     CDS             complement(2746..3189)
                     /locus_tag="PLQ29_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86206.1"
                     /translation="MPHVIHYEDDIFIIDALVRTLCDASRLEPDPDVLGDAMLSIAKL
                     ADASLRKARDLVLLNEHQVDRPESLRLLARTTRSFSDALSELIRHDAPLGQHLASSSD
                     ELARMASAHRAAASELADVLRQATGEDASPEDHVSGDELSELLRL"
     gene            3349..3630
                     /gene="rpsF"
                     /locus_tag="PLQ29_05850"
     CDS             3349..3630
                     /gene="rpsF"
                     /locus_tag="PLQ29_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPG86207.1"
                     /translation="MRSYELTVIFPTEEELYRQAKEKVSAALKEQGAEIVKEEELGDR
                     QLAYEIKDRMRGRYVLYVATIAPDKIVAVEKVLKLEPNVMKYLFVRAED"
     gene            3644..4120
                     /gene="ssb"
                     /locus_tag="PLQ29_05855"
     CDS             3644..4120
                     /gene="ssb"
                     /locus_tag="PLQ29_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPG86208.1"
                     /translation="MADLSVAVLVGRLTRDAELKYTNSGQAVCHFSVATSSRKKKGDQ
                     WVDEASFWDVDLWGKQGESLNQYLVKGKLVAVEGTMRQDRWEQDGQSRMKVLISANTV
                     QLLGGNTSGGQQYGASQGSQDTQGGPREYQGRSGSRPQGPAAQAPASDDFSDDIPF"
     gene            4155..>4371
                     /gene="rpsR"
                     /locus_tag="PLQ29_05860"
     CDS             4155..>4371
                     /gene="rpsR"
                     /locus_tag="PLQ29_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPG86209.1"
                     /translation="MSDMLNRDDRGSRNDEGRRDGDDRGGRGGRKGFFRKKVCKFCSQ
                     KLKIDYKDPETLKRFITERGKILPRRIT"
BASE COUNT          803 a         1404 c         1440 g          724 t
ORIGIN      
        1 gatggcggcg gtgtgcgtca cgttgccgcc cgcgtcgagg aaaaaagccg tattcgtcgg
       61 atagggcgtg gcgacgacga ccgtggtgcc cgacaggcgc agcgacggcg tatcataggc
      121 gctcgtctca ggcgcgctga gcgagaagct ccgctcggcg cgctcaccag ccttgtcgat
      181 caggataagg cgataggtgc cgcgcggtat cgtcgtcccc ggggcgtcga gcgaattagc
      241 cccgagccag acgctcgagc cgtcgtccga gcggcgccac tcggacggcc ccatcgtcca
      301 gcacagctcg tcgccgtcgt ggacgacgta caggtactcg acgtcgccga cgccctgggc
      361 gtcggcgacc gaggcgaaca ccgacaggcg ctcgtccctg gcgccgcccg gatcggtcgt
      421 cacgacgatc agccgaaccg ccaacgatac gatctccggt ggcgacgagg agcaggagac
      481 ggccagcgcc agcgccacgg ctgacgacaa cgtcgccgcg agggtcggtc cgagccgccg
      541 caagcgctca gtcccccagc gcggcgatca ccgagcccag gtcggcgtgc tcggcgttcg
      601 acctggccgc caggtcgcgg agcagccaca gcccacgcgc cttatcgtcg gaaccgcgta
      661 tcaacgtgta cgacgcgccc ttctgctcgg cgtaggcgta ctgcagggcc agcttgcgcg
      721 gttcgggata cacctcgcag gcaattccgg cgtcgcggag cacggcggcg gcggcctggt
      781 actctggcgt cagggccggc tcgacgttgg cgacgaggac gcgggtataa cccgccttgg
      841 cgcctgaacg tccgagctcc tcgagggcgg ctatcaggcg gtcgatgcct acggaggcgc
      901 cgacgccggg caggcgctgc ttcgtgtaca gcgaggcaag atcgttgtaa cggccgcccg
      961 aacagaccga gcctatgccg ggcaactcgc ggaggaaggt ctcaaagacg acaccggtat
     1021 agtagtcgag gccccgggtg atgcttgggc acagttctag cgagcttgag agcccgcagg
     1081 cctcggccat cgagaaaatc tcggacagac gatcggagtc ggggttcggg ccgcctgcca
     1141 gtcgcgtcat gcgatcgagg gtggggccga aggcctcgcc gccggcgatg aaggccatga
     1201 tctcgtctgc cgcggcgacg ccgaccagct ccgtcagctg acgccgcgtc tcaggctcgc
     1261 ctatcttggc caacttgtcg actacgcgta gcgcctcgac ggacctgtcc gcgacgccgg
     1321 cacgctccag gaagcggttg aatataccgc ggtggttcac gcggatggtt gcgccgggca
     1381 cgccgagctc cgtcatcgat cgggcgatgg tcgccacgat gtcgaagtcc gccgaggcgg
     1441 tgtcgacgcc cacgatatcg aaatcgcact gcatgaactc gcgataccgc ccgcgctgcg
     1501 tgttctcgcc gcgccagacc ttggccatgt ggtagcgcct gaaaggcagg tacagatcgc
     1561 ccgagtgttc cgccatgaag cgggcgaagg ggacggtcag gtcgaagcgc atcgccacgt
     1621 cgcgccctcc gttatcagcg aagcggtaca cctgcttctc cgtctcgccg ccggccttac
     1681 cgagcagtat ctcggcgtac tcgaggacgg gcgtatcgat cggcacgaag ccgaagctcc
     1741 taaaggcgcc ggtgagcgcc tcgatcatcg cggcccgctc gatctcggct cgcggaagcg
     1801 agtccctgaa gcccttgagc actcgaggct gtattatttc ggccatgtat agcgtctcct
     1861 agctggtcta ggcccgatta tggcctgagc ggccatccct gcgcaagctg gccgctcggg
     1921 cgagggggcc ggccgcgaag cttgacagcg cgggcgccgt catgaatgat tctgccatca
     1981 tgctgtttgt catcgacgcg gggaatacga acatcgtcgg cggcgtctac gactccggac
     2041 gcctcgtgga tacgctcagg atgcgtaccg tccagggcaa aaccgaggac gagtatggcg
     2101 tcatcgtcag ggcggtcctg ctggaccggg gcatagaccc ctcgaccata tcgcgggtca
     2161 tcgtcagctc cgtcgtgcct caactgacgg cagcgatgac gaagatgtcg cgccacctgt
     2221 tcggcgtcga gccggtgctc gtgggaccgg cggtctacgc gaggctcccc gtcaccgtgg
     2281 tggcggccaa cgagataggc tcggacctgg tcgccgacgc cgtggccgcc tggaaccgtt
     2341 tcggcggcgc ctgcctggtc gtcgatttcg gcaccgcgct gaccttcacg gccatcgacg
     2401 ccaaggccgc catcataggc gtatcgatag cccccggact gggtaccgcc gcagggtccc
     2461 tttcgtcggc caccgcccag ttgccgctgg tgccgctcga ggcgccggcc tcggccatgg
     2521 gccgcaacac ggtggaggcc atccaggccg gcgtggtgct cggctacacc ggcctcgtcg
     2581 agtacatggt cggccgcatc aagggagaac tcggcggagc caaggtggtc gcgaccggcg
     2641 gcctctgccg cgtcgtctcc gagctgacgg aggtgttcga cgccatcgac cccgacctga
     2701 ccctcaatgg gctcgccgag atggaacgct acctgttcta ggctgtcaca ggcgcagcag
     2761 ctccgataat tcgtcgcccg acacgtgatc ctcgggcgac gcgtcctccc cggtagcctg
     2821 acgcagtaca tcggccagct ctgaagccgc cgctcggtgg gccgacgcca ttctggccag
     2881 ctcgtccgac gaactggcta ggtgttgccc tagcggggcg tcgtgtcgga tcagctccga
     2941 tagcgcgtcg gaaaacgacc tggtcgtcct ggccagcagt ctgaggctct ccggcctgtc
     3001 gacctggtgc tcgttcagca gcacaaggtc gcgcgcctta cggagcgacg cgtcggctag
     3061 cttcgctatg gacagcatcg cgtcgccgag cacgtcggga tcgggttcca gccgcgaggc
     3121 gtcgcatagg gtacgcacca gggcgtcgat gataaagata tcgtcctcgt agtgtatgac
     3181 atgcggcatg gagttattgt aacgctgcca cgcgccaggg taaagcgtct cctaagcctt
     3241 gataccgtcg gcctttccgg tatatagtag cttgatttgc gcgcccatcg gggcgcgcga
     3301 ctccttatcc gtcctttcgg gcggatcata agaccatagg gaggatcgat gcgatcttac
     3361 gaactcacag ttattttccc cacggaagag gaactctacc gccaggccaa ggaaaaggtc
     3421 tccgccgctc ttaaagagca gggcgccgag atcgtcaaag aggaagagct cggcgaccgc
     3481 cagctcgcct acgagatcaa ggacaggatg cgcggccgct acgtgctgta cgtcgccact
     3541 atcgcgcccg acaagatcgt ggccgtcgag aaggtactca agctcgagcc gaacgtcatg
     3601 aagtacctgt tcgtcagggc agaagactaa cgggagggac gcgatggccg atctcagcgt
     3661 agccgtgctc gtaggcaggc ttacccgcga cgcggagctt aaatatacca actccggtca
     3721 ggccgtctgt catttctcgg tagccaccag ctcgaggaag aaaaagggcg accaatgggt
     3781 cgacgaagcc agtttctggg acgtcgacct ctggggcaag cagggcgaat ccctcaacca
     3841 gtacctggtc aagggcaagc tcgtcgccgt agagggcacg atgcgccagg atcgctggga
     3901 acaggacggc cagtcccgca tgaaggtact gatcagcgcg aataccgtac agctgctcgg
     3961 gggcaacacc tctggcggcc agcaatacgg ggcttcgcag gggagccagg atactcaggg
     4021 cgggccgagg gaataccagg gccgttcagg cagccggccg caaggacccg ccgcccaggc
     4081 tccggcgtcc gacgattttt cggacgatat accgttctag gctagaacgg gactcatacg
     4141 taaggagaca ctctatgtca gatatgctga acagggacga tcgcggctcg cgtaacgacg
     4201 agggccggcg cgacggcgac gaccgcggcg gccgcggcgg ccgcaaaggc ttcttccgca
     4261 agaaggtctg taaattctgc tcgcagaagc tcaagatcga ctataaggat cctgagacgc
     4321 tcaagcgttt catcaccgag cgcggcaaga tactgccccg taggataacc g
//
LOCUS       DAOPHV010000174         4361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_27241_length_4361_cov_3.542963, whole genome shotgun sequence.
ACCESSION   DAOPHV010000174 DAOPHV010000000
VERSION     DAOPHV010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27241_length_4361_cov_3.542963"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..676
                     /gene="hslV"
                     /locus_tag="PLQ29_05865"
     CDS             140..676
                     /gene="hslV"
                     /locus_tag="PLQ29_05865"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HPG86210.1"
                     /translation="MNRIRSTTIIAVRRGGKVAMAGDGQVTMGETVMKSNARKVRTIY
                     DGRILTGFAGATADAFTLLEHFEVKVKEYQGDLTRAAVELAKDWRTDKMLRKLEALLL
                     VADREKTLLLSGTGDVIEPAGDAIAIGSGGNYAYAAALAYLDTSELSAAEIARRSLGI
                     AGGICIYTNDHILVEELP"
     gene            673..2088
                     /gene="hslU"
                     /locus_tag="PLQ29_05870"
     CDS             673..2088
                     /gene="hslU"
                     /locus_tag="PLQ29_05870"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HPG86211.1"
                     /translation="MTEDFKTFTPRRVVEELDRYIIGQDKAKRAVAIALRNRLRRKLL
                     PEAAREEIAPKNILMIGPTGVGKTEIARRLARLCGAPFVKVEATKYTEVGYVGRDVES
                     MIRDLMAAGYRMVVEEMAAKVKEDAERHTEERLLDLLLPGLGQKPAAPTASASAKPET
                     WATPVVEPPVAQVAASKGSDTREKFRAMLREGLLDGKEVEVQVSAQSAPSIELFAGQQ
                     MEELESAFSGIANIFGGKKKKKTVTVKEARRVILEEESDRLVDKDRASAEARERVEQS
                     GIVFIDEIDKIATREGRSGGGMDVSREGVQRDILPIVEGSKVTTKYGVVDTTHILFIA
                     AGAFNVSKPSDLIPELQGRFPIRVELEALSASDFVRILTEPENALVTQYTQLLGTEGV
                     TLTFSAEAISRLASIAEKANARTENIGARRLHTIMERLLEELSFDAPERGGDSVEIGE
                     AFVEERFKGVFESEDLAKFIL"
     gene            2276..2695
                     /gene="flgB"
                     /locus_tag="PLQ29_05875"
     CDS             2276..2695
                     /gene="flgB"
                     /locus_tag="PLQ29_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HPG86212.1"
                     /translation="MLENTTFGRTVELLQRSMDVASLRHEVIGNNIANAEVPNFKRSE
                     INFESSLKRAIDSERREPRLQLATTEPGHIASWKRADWRDVSPRRVLDYLSTTKNNGN
                     NVDAEQEFMDAMTNQLRYTLMTQAAAFEFNQVNQVLK"
     gene            2705..3163
                     /gene="flgC"
                     /locus_tag="PLQ29_05880"
     CDS             2705..3163
                     /gene="flgC"
                     /locus_tag="PLQ29_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HPG86213.1"
                     /translation="MGMFTSINIAASGMTAERLRQDVIADNIANQSTTRTPEGGAFRR
                     SRVVLRPKTDTPYFRLPFLPDQWDGGAGEGVRVSEIQKDYAAETRLVYDPTHPDAIKT
                     GPRTGYVEYPNVNVVTEMVDLISASRAYEANAAIANGAKAMFMKALEIGR"
     gene            3167..3562
                     /gene="fliE"
                     /locus_tag="PLQ29_05885"
     CDS             3167..3562
                     /gene="fliE"
                     /locus_tag="PLQ29_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HPG86214.1"
                     /translation="MSTLFGVPGVSNQIGIQSVAGGAMTGATPIGGDRLKLSRLDPRH
                     FATGGGTPALGDEGKASFEDAMLKAMDGVNAMQMDSSAAMETMLIDPDSIDAHDVTMA
                     MAKANMSLNITRTIMDRVVRAWKDIINTR"
     gene            3613..>4361
                     /gene="fliF"
                     /locus_tag="PLQ29_05890"
     CDS             3613..>4361
                     /gene="fliF"
                     /locus_tag="PLQ29_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HPG86215.1"
                     /translation="MNEWLKKTAERLKTLWGSWGLVQKLILIGVGLAVVVGIVLLVSV
                     SGAPTQVRLINTPIKSDEELRLITSRLDEEGIGFSLGEGNVLYVKDEATRSRANAILL
                     REDLIPRGTDPWAVFSIDKFTVTDFERNVNLRKAITAEVTRHIESIADVDKANVVIEI
                     PEDKLFAADQKSVTASIIIFPKPGSDIVTNRKKLEGIQKIVRFAVSGLTDDNIVITDQ
                     NGLQLNDFAGLADFDRIERTKREQQLIQKQEA"
BASE COUNT          929 a         1317 c         1452 g          663 t
ORIGIN      
        1 ggaatatatc gaccacgcag gtgtatacgc acgtggacat ggcgcgccta cgcgaggttt
       61 acgacggggc ccatccgcac gccggaaagc cgcgcggggc ttcgggcgaa gcgagcgaca
      121 cgattagggg aacgacgaga tgaacaggat acgaagcacg acgatcatag ccgtcagacg
      181 cggcggcaag gtcgcgatgg ccggcgacgg ccaggtgacg atgggcgaga ccgtgatgaa
      241 gagcaatgcc aggaaggtcc gtaccatcta cgacggcagg atactgaccg gtttcgccgg
      301 agccaccgcc gatgccttta ccttgctcga acacttcgag gtcaaggtca aggagtacca
      361 gggcgacctg acgagggccg ccgtcgaatt ggccaaggac tggcgcacgg acaagatgct
      421 acgcaagctc gaggcgctgc tcctggtagc cgatcgggag aagaccctgc tcctctcggg
      481 taccggcgac gtgatagaac cggccgggga cgccatcgct ataggctcgg gcggcaacta
      541 cgcctacgcc gctgccctcg cgtacctcga cacgagcgag ctgtcggccg ccgagatagc
      601 caggcgcagc ctcggcatcg ccgggggcat ctgcatctac acgaacgacc atatcctcgt
      661 ggaggaactg ccgtgaccga ggacttcaag accttcacgc ccaggcgcgt cgtcgaggag
      721 ctggaccgct acatcatcgg ccaggacaag gccaagcggg ctgtcgcgat cgccctgcgt
      781 aaccgcctcc ggcgcaagct gctgcccgag gcggcccgcg aggagatagc ccccaagaac
      841 atcctgatga taggccccac cggcgtcggc aagaccgaga tagccaggcg gctggccagg
      901 ctctgcggcg cgcccttcgt caaggtcgag gcgaccaagt acaccgaggt cggctacgtc
      961 ggccgtgacg tcgagtcgat gatacgcgac ctgatggccg ccggttatcg catggtcgtc
     1021 gaggagatgg cggccaaggt caaggaggac gccgagcgcc acaccgagga gcgcctgctc
     1081 gacctgctcc tgccagggct tggccagaag ccggcggcgc cgaccgcatc ggccagcgcc
     1141 aagccggaga cttgggcgac tccggtcgtc gagccgccgg tcgcccaggt cgcggcctcg
     1201 aagggctccg acacgcggga gaaattccgc gccatgctgc gcgaaggcct cctcgacggc
     1261 aaggaggtcg aggttcaggt ctccgcccag tccgcgccgt cgatcgagct gttcgccggg
     1321 cagcagatgg aggagctcga gtcggccttc tccggcatcg cgaacatctt cggcggcaag
     1381 aagaagaaga agacggtgac cgtcaaagag gccaggcgcg ttatcctcga ggaggagtcg
     1441 gaccgtctcg tcgacaagga ccgggcctcg gccgaggcgc gcgagcgggt cgagcagtcg
     1501 ggcatcgtgt tcatcgacga gatagacaag atagcaaccc gcgaggggcg ctccggtggc
     1561 ggcatggacg tctcgcgcga gggcgtgcaa cgggacatcc tgcccatcgt cgagggctcg
     1621 aaggtcacca ccaagtacgg cgtcgtcgat acgactcata tcctgttcat tgcggccggc
     1681 gcgttcaacg tgtccaagcc gtccgacctg atccccgagc tccaggggcg cttcccgata
     1741 cgcgtggagc tcgaggcgct gtcggcgtcg gatttcgtgc gcatcctgac cgagccggag
     1801 aacgcgctcg tcacgcagta tacgcagtta ctcggtaccg agggcgtgac cctgacgttc
     1861 tccgccgagg cgatcagtcg cctggcgtcg atagcggaga aggcgaacgc gcgcaccgag
     1921 aacatcggtg ccaggcgcct gcatacgatc atggagcgcc tgctcgagga gctgtccttc
     1981 gacgcgccgg agcgcggcgg cgactcagtg gagataggtg aagccttcgt cgaggagcgc
     2041 ttcaagggcg tcttcgagag cgaggacctc gcgaagttca tattgtaggt gtaggtaagc
     2101 cagcgaagag gatacgaggt cgagagggaa gaaagagaag agggagaatg agggctatga
     2161 aggggatgga tccttgagct tcctcttcgt tcaattggag taacaagccg gcggtaatgg
     2221 gccgataaga gaaggtgagg gcctccggag aacggaggcc agtggaggcg gatcgatgct
     2281 cgagaatacg acgttcggta ggacagtgga actgctccag cgtagcatgg acgtggcgtc
     2341 cctgcgccac gaggtcattg gcaacaacat cgccaacgcc gaggtgccga atttcaagcg
     2401 ctcggagatc aacttcgaat cgtccctgaa gcgcgccata gactccgaga ggcgcgagcc
     2461 cagactacag ctggcgacca cggagccggg ccatatcgcg tcgtggaaac gggccgactg
     2521 gagggacgtc agcccgcgca gggtgctcga ctacctgtcg acgaccaaga acaatggtaa
     2581 caatgtcgac gccgagcagg agttcatgga tgcgatgacg aaccagctgc ggtataccct
     2641 gatgacccag gccgccgcct tcgagttcaa ccaggtcaac caggtcctga aataagaggt
     2701 acatatgggc atgttcacga gcatcaatat cgcggcgagc gggatgacgg cggagcggct
     2761 ccgccaggat gtcatcgccg acaacatcgc caaccagtcg acgacccgca ctcccgaggg
     2821 cggagcgttc aggcgtagcc gcgtggtatt acggcccaag acggataccc cgtatttccg
     2881 gctaccgttc ctaccggatc agtgggatgg cggcgccggc gagggcgtgc gagtgtcgga
     2941 gatccagaag gactacgccg ccgagaccag gctcgtctac gacccgaccc acccggacgc
     3001 gatcaagacc ggcccccgga ccggctacgt cgagtacccg aacgtcaacg tggttaccga
     3061 aatggtcgac ttgatatccg cgtcgcgagc ctacgaggcg aacgcggcga tagccaacgg
     3121 cgccaaggcc atgttcatga aggctttgga gataggaagg taagtgatgt cgaccctgtt
     3181 cggcgtcccg ggcgtctcga accagatagg catccaatcc gtggccggcg gagcgatgac
     3241 cggagcgacc ccgatcggcg gggatcggct gaagttgtcg aggctcgacc cgaggcactt
     3301 cgcgaccggc ggcggcacgc ccgccctcgg tgacgagggt aaggccagct tcgaggatgc
     3361 gatgctcaag gccatggacg gcgtcaacgc catgcagatg gacagctccg ccgccatgga
     3421 gacgatgctg atcgatcccg attccataga cgcgcacgac gtgaccatgg cgatggccaa
     3481 ggccaatatg tctctgaaca tcaccagaac catcatggac cgcgtggtcc gggcgtggaa
     3541 ggatattatc aatacgagat gacggcaggg ccttgatggc tcggcgtcgt tcgttcttct
     3601 gggaggggga acatgaacga atggctaaag aagaccgcgg agcgcctgaa gaccctttgg
     3661 ggctcctggg ggctcgtcca gaaactgatc ctgatcggag tcggcctggc cgtcgtcgtg
     3721 gggatcgtgc tgctcgtctc ggtgtccggc gcgcctacgc aggtgcggct gatcaacacg
     3781 cccatcaaga gcgacgagga actcaggctc atcacgagtc gcctcgacga ggagggcatc
     3841 ggcttctcgc tgggcgaggg caacgtgctc tacgtcaagg acgaggcgac gcgctcgcgg
     3901 gccaacgcga tactgctccg ggaggatctc atcccgaggg gcaccgatcc ctgggcagta
     3961 ttctccatcg ataagttcac ggtcaccgac ttcgagcgca acgtcaacct ccgcaaggcc
     4021 atcacggccg aggtgaccag gcatatcgag tcgatagccg acgtcgacaa ggcgaatgtc
     4081 gtcatcgaga tacccgagga taagctgttc gccgccgacc agaagagcgt gacggccagc
     4141 atcatcatct tcccgaagcc cggctcggac atcgtgacca accgaaagaa gctcgagggc
     4201 atccagaaga tcgtgcgctt cgcggtctcc ggcctgaccg acgacaacat cgtcatcacc
     4261 gatcagaacg gcctgcagct gaacgacttc gcagggctcg ccgacttcga ccgcatcgag
     4321 cggaccaagc gggagcaaca gctgatccag aagcaggagg c
//
LOCUS       DAOPHV010000175         4353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_27315_length_4353_cov_3.727082, whole genome shotgun sequence.
ACCESSION   DAOPHV010000175 DAOPHV010000000
VERSION     DAOPHV010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27315_length_4353_cov_3.727082"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..1235)
                     /locus_tag="PLQ29_05895"
     CDS             complement(204..1235)
                     /locus_tag="PLQ29_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPG86216.1"
                     /translation="MMKRLMIAAFAATIGLVASACGGSSEKLYLFNWIYYIPEDVIED
                     FSKETGIQVIVDSYASNEEMYNKIVGGGDGYDIVVPSGDYVSIMIAEGLLEPIDTSRL
                     ANYGNLDPAAVARIGFDPGNRYSVPFMMAAAGVSVNRTKVSGYEHSWSVFDRSDLGGR
                     MTMLDDMREVLGAALKSLGYSVNDPDPAHIEEARRVVERWKPNLVKFDAESFAKSFAS
                     GEFWVVQGYAENVFIEYPEARRAEVDFFFPKEGMPMYMDSFCVLRGAKNVERAYAFID
                     YMLRPDVAARVADYLMLPSPNVPARALMKVEPNYDFDDLARGEFKEDLGREALKLYNE
                     AWRRIRVGN"
     gene            complement(1267..2061)
                     /locus_tag="PLQ29_05900"
     CDS             complement(1267..2061)
                     /locus_tag="PLQ29_05900"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG86217.1"
                     /translation="MSRPAARKRSSFSRSVFVGTIVFLSLPLLVLVLYSFNESKSGAW
                     TGFSVKWYVKLFTDSPQLWRAFGNSAVVALASAFVATVIGTLGAVAVNWYRFRLRKYL
                     RTLTFMPMILPEIIIGVSLLVFFSGIGLRLSLFTVFVAHVSFNLPFVFLLVLARLEEF
                     DFSIIEAARDLGASDGQTLLRVILPISAPGIISGFLTAVTLSLEDFVITFFVTGPGAT
                     TLPLYVYSAIRFGVSPVINALSVVMILGTVLLTWSTRNFLKYIAAK"
     gene            complement(2058..2942)
                     /locus_tag="PLQ29_05905"
     CDS             complement(2058..2942)
                     /locus_tag="PLQ29_05905"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG86218.1"
                     /translation="MRREPGGLYAAPSTIWLTVFFLAPLAIIVAYSFLTPGARGGVEW
                     KPTLDAYRAIANPTFAKVTLNTILVAIGATAITLLIALPCGYYMARSERQTLLLMLVM
                     IPFWTNFLIRIYAWIAILGNEGFLNDILLALGLVDESVQFLYNRGAVMLVLVYTYLPY
                     AILPLYSTIDKFDFSLLEAARDLGASKLGAITRVLLPNVKGGILTAFLFTFIPIFGAY
                     AVPLLVGGTESYMLGNLIADELTKSRDWPLASAISLVITLVTTIGVLLLLRANGASTL
                     GGAREAKAEPEPAFGGAR"
     gene            complement(2939..4045)
                     /locus_tag="PLQ29_05910"
     CDS             complement(2939..4045)
                     /locus_tag="PLQ29_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG86219.1"
                     /translation="MKGSDVVIEHVRKSFGEFKALDDVSIDIRKGEFFSLLGPSGCGK
                     TTLLRIIAGFESPEGGAVLLDGEDVVGQAPDARHVNTVFQNYALFPHLSVFENVAFSL
                     RLRRTPESEVKARVEEYLRLVELQAHASKKPAQLSGGQKQRVAIARALINEPSVLLLD
                     EPLSALDAKLRQHMLIELDSIHDKIGITFIYVTHDQQEALSVSDRIAVMNAGEVLQVG
                     TPQEIYEAPATDFVAKFIGETNLFEADVASVSGEMVELRTDGFGPVKVTATEPPPVGS
                     RVSWTIRPEKIRIGLEEPRSARNGINAVAGVVDEPIYSGFQSKFYVRAPSGLVLKVIK
                     QHADWSDEGPEIAWKDAVWLSFSAEDAYIVEVKR"
BASE COUNT          809 a         1359 c         1425 g          760 t
ORIGIN      
        1 tcgcgcaaat taagcttgag cgcgccaaat ctcacgtcaa ggccgccgtc gcgatagcga
       61 actgagtccg cgaagatgga cgaagaggat acgactccaa gagtcgggca agaggaggag
      121 agagagtaga tcagcgaatc cccctctctc tcctctcgcg tatctcgtat cctccagttc
      181 tctatgcctg gcttgaacga cgatcagttg cctacgcgga tacggcgcca ggcctcgttg
      241 tatagcttga gcgcctcgcg gccgaggtct tccttgaact cgccgcgggc caggtcgtcg
      301 aagtcgtagt tcggctcgac tttcatcagg gcgcgggccg gcacgttcgg cgagggcagc
      361 atcagatagt cggcgacgcg cgcggcgacg tcgggcctga gcatgtagtc gatgaacgcg
      421 taggcccgct cgacgttctt ggcgccccta agcacgcaga agctgtccat gtacatcggc
      481 atgccctcct tcgggaagaa gaagtcgacc tcggcgcgcc tggcctccgg gtactcgatg
      541 aacacgttct cggcgtagcc ctgcactacc cagaactcgc cggaggcgaa gctcttggcg
      601 aaggactcgg cgtcgaactt gaccaggttc ggcttccagc gctcgacgac gcggcgggcc
      661 tcctcgatat gggccgggtc ggggtcgttg accgagtagc ccagggactt gagcgccgct
      721 ccgagcacct cgcgcatgtc gtcgagcatg gtcatgcggc cgccgaggtc cgatcggtcg
      781 aagacgctcc agctatgctc gtagccggaa accttggtcc ggttcaccga gacgccagcc
      841 gcggccatca tgaaaggcac gctgtaccgg ttgcccgggt cgaagccgat gcgcgcgacc
      901 gccgcggggt ccaggttgcc gtagttggcc agcctggagg tatcgatggg ctcgagcagc
      961 ccctcggcta tcatgatcga cacgtagtcg ccggagggca cgacgatgtc gtagccgtcg
     1021 ccgccgccga ctatcttgtt gtacatctcc tcgttcgagg cgtacgagtc gactataacc
     1081 tgtatacccg tctccttcga gaagtcctcg atgacgtcct cgggtatata gtagatccag
     1141 ttgaacaggt acagcttctc ggaggagccg ccgcaggcgg acgctaccag gcctatcgtc
     1201 gcggcgaacg ccgcgatcat caatcgcttc atcatcgtca ttgcctccaa tcgacggacg
     1261 gacggcctac ttggccgcga tgtacttgag gaaattgcgc gtggaccagg tcaggagcac
     1321 ggtgccgagg atcatcacga ccgacagcgc gttgatcacc ggtgacacgc cgaagcgtat
     1381 cgccgagtac acgtagaggg gtagcgttgt cgctcctggg ccggttacga agaacgtgat
     1441 aacgaagtcc tcgagcgata gagtgaccgc ggtcaggaag cctgaaatga tgcccggcgc
     1501 cgagatcggt aggatcacgc ggagcaaggt ctggccgtcg ctcgcgccca ggtcgcgcgc
     1561 cgcctcgatg atcgagaagt cgaactcctc gagcctggcg agcacgagca ggaacacgaa
     1621 gggcaggttg aacgacacgt gcgccacgaa gaccgtgaac agggaaaggc gcaggccgat
     1681 gcccgagaaa aagaccagga gcgacacgcc gatgatgatc tccggcagga tcatcggcat
     1741 gaaggtcagc gtccgcaggt acttccgcag gcggaaacgg taccagttga ccgccaccgc
     1801 gccgagcgtg cctatgacgg tggcgacgaa ggccgaggcc agcgccacga ccgcgctatt
     1861 gccgaaggcg cgccacagct gcggcgagtc ggtgaacagc ttgacgtacc acttgaccga
     1921 gaagccggtc caggcgccgg acttggactc gttgaacgag tacagcacca gcacgaggag
     1981 gggcaggctc aggaacacga tcgtaccgac gaacacgctc cgcgagaagc tcgagcgctt
     2041 ccgcgccgcg gggcggctca tcgggcgccc ccgaacgccg gctccggctc ggctttcgcc
     2101 tcccgggcgc cgccgagcgt ggaggcgccg ttggcccgca ggagcaggag cacgcctatc
     2161 gtcgtcacca gcgtgatgac gagggagatc gccgaggcga gcggccagtc ccggctcttg
     2221 gtcagctcgt cggctatcag gttaccgagc atgtacgact cggtgccgcc gacgaggagc
     2281 ggcaccgcgt aagcgccgaa gatggggatg aaggtgaata ggaaggccgt gagtatcccg
     2341 cccttgacgt tcggcagtag caccctggtg atcgcgccta gcttggacgc ccccaggtct
     2401 cgggccgcct cgagcagcga gaagtcgaac ttgtctatcg tcgagtacag gggcaggatc
     2461 gcatacggca ggtaggtata gacgagcacc agcatcacgg cgccgcggtt gtacaggaac
     2521 tgcacgctct cgtccacgag gccgagcgcg aggaggatgt cgttcaagaa gccctcgttg
     2581 ccgagtatcg ctatccaggc gtagatgcgg atcaggaagt tggtccagaa cggtatcatc
     2641 acgagcatca ggagcaaggt ctggcgctcg ctcctggcca tgtagtagcc gcagggcagg
     2701 gcgatgagca gcgttatggc cgtggcgccg atcgcgacga gtatcgtgtt cagcgtgacc
     2761 ttggcgaagg tcgggttggc tatcgcgcgg tacgcgtcca gggtcggctt ccactcgacg
     2821 ccgccccggg cgccgggcgt gaggaagctg tacgcaacga tgatcgcgag cggggcgagg
     2881 aagaagacag tcagccagat cgtcgacggg gccgcgtaca ggccgccggg ctcgcgcctc
     2941 atcgcttgac ctcgacgata taggcgtcct cggcgctgaa cgatagccaa acggcgtcct
     3001 tccaggcaat ctccggcccc tcgtcggacc agtcggcgtg ctgcttgatc accttgagca
     3061 cgagaccgct cggcgcgcgc acgtagaact tgctctggaa gccggagtag atcggctcgt
     3121 cgacgacgcc ggccacggcg ttgatgccgt tcctggccga cctgggctcc tcgaggccga
     3181 tgcgtatctt ctccgggcgt atcgtccagc tgacgcggga gccgaccggg ggcggctcgg
     3241 tcgccgtcac cttgaccggg ccgaagccgt cggtccggag ctcgaccatc tctcccgaca
     3301 ccgacgcgac gtcggcctcg aagaggttgg tctcgcctat gaacttggcg acgaagtcgg
     3361 tggcgggcgc ctcgtagatc tcctggggcg tgccgacctg cagcacctcg cccgcgttca
     3421 tcacggcgat gcggtcggag acggacaggg cctcctgctg gtcgtgcgta acgtagatga
     3481 aggtgatgcc tatcttgtcg tggatggagt cgagctcgat cagcatgtgc tggcgcagct
     3541 tggcgtcgag ggccgacagg ggctcgtcga gcagcagcac cgatggctcg ttgatgagag
     3601 ccctggctat cgccacgcgc tgtttctggc ccccggacag ctgggccggt ttcttggagg
     3661 cgtgggcctg gagctcgacg agccgcaggt actcctcgac cctggccttg acctccgact
     3721 ccggcgtcct acggaggcgc agcgagaagg ccacgttctc gaacacggat aagtgcggga
     3781 acagggcgta gttctggaag acggtgttca cgtgccgggc gtccggcgct tggcctacca
     3841 cgtcctcgcc gtcgagcagc accgctccgc cttcggggct ctcgaagccc gcgatgatgc
     3901 gcagcaaggt cgtcttgccg cagccggatg gacccaagag cgagaagaac tcgcccttcc
     3961 tgatgtcgat gctgacatcg tccagcgcct tgaattcgcc gaacgacttg cgtacgtgct
     4021 cgatgactac gtcgctccct ttcaccgtct tttgctttac ccctccgcgt tccatgatgc
     4081 cgcctgagtg gcggccgttt ccggagaccg ataccggccc ggggcggaat acgatccgcc
     4141 tgaatcgatc atagacgagc gcacaatgcg gcattcgggg ccggcgtgtc aatcggttgg
     4201 cgaccatgta tacccgctat atgcctccat tattcacggt ttgtcgcggt gaagctcatt
     4261 ttatacatat ttcatgcacg cggcatccag cccctggccg ccccgaacga tcgcccggcc
     4321 cttgcgcaag ccggtcggcc tcgccacaat gcc
//
LOCUS       DAOPHV010000176         4349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_27356_length_4349_cov_3.177224, whole genome shotgun sequence.
ACCESSION   DAOPHV010000176 DAOPHV010000000
VERSION     DAOPHV010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27356_length_4349_cov_3.177224"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1103
                     /gene="lepB"
                     /locus_tag="PLQ29_05915"
     CDS             138..1103
                     /gene="lepB"
                     /locus_tag="PLQ29_05915"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPG86220.1"
                     /translation="MDARTDFLASLESFTERALTRRKRRRLAQKKKQQAKHWVRDWAE
                     AILWAVCMVLLINQYLFQMYRIPSGSMSGTLEIGDMIFVNKLVYGPELLPGVAKMPGF
                     REARRGEVVVFENPAYLSKGPVFTILQQFVYMATLTLVDIDLDELGQPRVHYLIKRAV
                     AVGGDTVRVADGELSFRFQGDDEWITERDFQELSGFSYPLRRMVSADEYPAIHEAGRV
                     LAAMDMGLADSAPALRYSPYDEQALLASRYAELAAAYPHDSRFVSEDRKARSGWYIPE
                     DRIFTLGDNRDNSKDARWFGAVSLDKVLGHALFKYWPLGRVGGIR"
     gene            1106..1834
                     /gene="lepB"
                     /locus_tag="PLQ29_05920"
     CDS             1106..1834
                     /gene="lepB"
                     /locus_tag="PLQ29_05920"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPG86221.1"
                     /translation="MKRVQSYEDARRRRSSALRFLRYVAFLYLGFALMVTFGLKTYTV
                     GSRAMRPALEPKDKILVASSAYGVVNPFNGQRLGFRSPERGDVALMKLPAAPTRGWRT
                     RLADSVIGFLTLQRVGSPGADDSLDSPVVKRVIACPGDSVSMSGYVMRVKVAGSEHFL
                     TEYEVSGQDYDIVSSGPGDGWSTSLPLSGEMPAMELGPGEYFVVDDDRSYCSDSRFFG
                     PVRAERFVGKVLLRYWPLDRITGL"
     gene            1845..3041
                     /gene="hemW"
                     /locus_tag="PLQ29_05925"
     CDS             1845..3041
                     /gene="hemW"
                     /locus_tag="PLQ29_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPG86222.1"
                     /translation="MAVAPSLYVHVPFCASRCAYCDFYSSTPSGPALESAAGRWLRAL
                     RTHVEFLASRFGRDGFETIYIGGGTPSWLPGDTLAEALWLIGRAARSGGREPTEWSLE
                     ANPEDVDERLIDTLLEAGVTRLSVGAQSLEEEARRLSARRGTAAETLGRLEVIAARWP
                     GRWSVDLMFGLPGQSAEGLAEDARTVIGQGAGHVSLYELTLEDGTPLSRAAASGTVSL
                     PDDDQRADHYEAARSRLLEAGFRRYEVSNWALDGQECAHNLRYWNLDDWLAIGPSGVG
                     NVASGPGAFRRIVNAADLDAYCSDPSGSASESTVDGKEAAFECLMMALRTSRGLSLGS
                     FERRFGMKAMDAFGPLHDRFPGLVRLEGDAWIATDRGLDTLNEPLVDALSSIERSWAG
                     RSPQPV"
     gene            3038..3892
                     /gene="pdxY"
                     /locus_tag="PLQ29_05930"
     CDS             3038..3892
                     /gene="pdxY"
                     /locus_tag="PLQ29_05930"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HPG86223.1"
                     /translation="MNILSIQSHVAYGHVGNRSAVFPLERLGYEVWPVNTVQYSCNAG
                     IPGWTGAAFGADHVADIITGLERVGAMSRCDAVLSGYVGDAETGRAILEAVSVAKRLN
                     PTTLYCCDPVMGDRPEGLYVRGELPDFFMDEAIPLADIVCPNVFEAELLSGIVIESDD
                     DLARATDAIHAKGPGVVMVTSYRPPSRDRIGLYVSDGRTKLAASAPIIRFDKPPKGSG
                     DLSSALFLGYYLIGGGVGEAIEKTLDALYSVLESSASGGGDNLAIVAAQDAIAEPGRR
                     FTATAPTG"
     gene            complement(3903..>4349)
                     /locus_tag="PLQ29_05935"
     CDS             complement(3903..>4349)
                     /locus_tag="PLQ29_05935"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HPG86224.1"
                     /translation="GLWKIIDDKTGKPNAYVMLYMNGGKLYGRMIATISLDTGLIDDT
                     LATKKFRADALAGDPPNCGLDFVYELQDKGKEWKGSIIDPADGKVYDCSIKREGSKLT
                     VRGSLKGTGGLLGRNQTWLLASASEMPTGFALPDPRTFAPVIPRKK"
BASE COUNT          716 a         1407 c         1421 g          805 t
ORIGIN      
        1 gccgaggacc ggcctgagcc gcacttcggc gtcgtctata tcctgttggg ttatcatagt
       61 atcgtcgttc atcgcgggat tatatgggcg cgcgccgagg cttgtcaacg cgaacgaaag
      121 atggtagcct cgagccgatg gacgcaagaa ccgacttcct cgcgtcgctg gagtccttca
      181 ccgagcgcgc cctgacgcgc cgcaagaggc gccggctcgc ccagaagaag aagcagcaag
      241 ccaagcactg ggtccgagac tgggccgagg ccatactatg ggccgtctgc atggtgctcc
      301 ttattaacca gtacctattc cagatgtacc gcatcccatc gggctcgatg tccggcacgc
      361 tcgagatcgg cgacatgatc ttcgtcaaca agctcgtcta cggtccggaa ctattgccgg
      421 gcgtggccaa gatgcccggg ttcagagagg cgcgccgcgg cgaggtcgtc gtcttcgaga
      481 acccggccta tctgtcgaag ggccctgtct tcaccatcct gcagcagttc gtctatatgg
      541 ctacccttac gctcgtcgac atagacctgg acgagctggg tcagccccgt gtccactacc
      601 tgatcaagcg ggccgtggcg gtcggcggcg acaccgtccg cgtggccgac ggcgagctga
      661 gcttcaggtt ccagggcgac gacgaatgga tcaccgaacg cgacttccag gagctgtccg
      721 gtttctccta tccactgagg cgtatggttt ccgccgacga gtatccggct atccacgagg
      781 cagggcgcgt actggccgcg atggacatgg gcctggccga tagcgctccc gccctgcgct
      841 atagccccta cgacgagcag gctctgctgg catcgcgcta cgcggagctg gccgcggcct
      901 atccccacga ctcgcgcttc gtgtccgaag acaggaaggc gcgctcgggt tggtacattc
      961 ccgaggacag gatcttcacg ctcggcgaca accgcgacaa ctcgaaggac gccaggtggt
     1021 tcggggcggt gtcgctcgac aaggtcctcg ggcacgcgct gttcaagtac tggccgctcg
     1081 gtcgcgtcgg cgggatacgg tagccatgaa gcgtgtccaa tcctacgagg acgccaggcg
     1141 ccgcaggtcc tcggccctta ggttcctgcg ctacgtcgcg ttcctgtacc tgggcttcgc
     1201 gctgatggtc accttcgggc tcaagaccta taccgtcggc tcgagggcca tgcgtccagc
     1261 gctcgagcct aaggacaaga tccttgtcgc ctcgtcggcg tacggcgtcg tcaatccctt
     1321 caatggccag cgcctcggct tcaggtcacc ggagcgtggc gacgtggcgc tgatgaagct
     1381 accggcggcg ccaacccgcg ggtggaggac caggcttgcc gattcggtaa tcggcttcct
     1441 gaccctccag cgcgtcggct cgccgggcgc tgacgactcg ctcgactctc ccgtcgtcaa
     1501 gcgagtcata gcctgcccgg gcgattcggt ctcgatgtcg ggttacgtca tgcgggtcaa
     1561 ggtcgccggt agcgagcatt tcctgaccga atatgaggtc tccggacaag actacgatat
     1621 cgtcagctcc ggcccgggcg acggctggtc cacgagcttg ccgttgtccg gggagatgcc
     1681 cgccatggag ctcgggcccg gcgagtactt cgtggtcgac gacgaccgct cctactgctc
     1741 cgattcccgc ttcttcgggc cggtcagagc cgagcgtttc gtcgggaagg tgttgctacg
     1801 ctattggcct ctcgaccgta taaccggctt gtaggataat ccccgtggcc gtcgcgcctt
     1861 cgctatacgt acacgttccg ttctgcgcct cccgttgcgc gtactgtgac ttctatagct
     1921 ctaccccgag cgggccggcg ctcgagtcgg ccgctggtcg atggctccgg gcgttgcgaa
     1981 ctcacgtgga gttcctcgcg tcgcgcttcg gccgcgatgg tttcgagacc atatacatag
     2041 gcggcggcac cccttcgtgg cttcctggcg atacgctcgc ggaggctctc tggcttattg
     2101 gtagggcggc ccgctcgggc gggcgggagc cgacggaatg gagcctcgag gcgaaccccg
     2161 aggacgtcga cgagcggctg atcgataccc tgctggaggc cggcgtgacc aggctgagcg
     2221 tcggagcgca gagcctcgag gaagaagcta ggcgcctgag cgcccgcagg ggaacggcgg
     2281 ccgagaccct cggtcggctc gaggtcatag ccgcccgttg gcccgggcgc tggtccgtcg
     2341 acctcatgtt cgggttaccc ggacagagcg ccgagggact ggccgaggat gcccggaccg
     2401 tgatcgggca aggggccgga catgtgtctc tgtacgagct aacgctcgag gacggtacgc
     2461 cgttgagccg cgcggctgcg tcgggaaccg tatccttgcc cgacgacgac cagcgagccg
     2521 atcattacga ggccgctcgc tcgcgtttgc tcgaggccgg tttcaggcgc tacgaggtgt
     2581 cgaactgggc gctcgacgga caggaatgcg cccataacct ccggtactgg aacctcgacg
     2641 actggctggc catagggcct tccggcgtcg gcaacgtcgc gagcggaccc ggagcctttc
     2701 gtaggatagt caacgccgcg gatctcgacg cctactgctc cgacccatcg ggtagcgcgt
     2761 ctgaatcgac cgtggatggc aaggaggcgg ccttcgaatg cctgatgatg gccctgagaa
     2821 cctctcgagg cctgtccctg ggctcgttcg aacgacgctt cgggatgaaa gcgatggacg
     2881 ccttcggtcc cctccatgat cgcttccccg gactcgtgcg cctcgagggc gacgcgtgga
     2941 tcgccaccga ccgcgggctc gatacgctca acgagccgct cgtcgacgcc ctgtcctcga
     3001 tcgagcgttc gtgggccggc cggagccctc aacccgtatg aacatcctat cgatacaatc
     3061 gcacgtggcc tacggccacg tcggcaaccg ctccgcagta ttcccgctcg aacgcctcgg
     3121 ctacgaggtc tggccggtca acaccgtgca gtactcgtgc aacgccggga ttcccggatg
     3181 gaccggcgcg gccttcggcg cggaccacgt cgccgatatc atcacaggcc tggaacgcgt
     3241 gggggcgatg agccgctgcg acgcggtgct ttcaggctac gtcggcgacg ctgaaacggg
     3301 acgcgccatc ctcgaggccg tctccgtcgc caagcggctg aacccgacga ccctctactg
     3361 ctgcgacccg gtcatgggcg acaggcccga aggcctctac gtacgcggcg agctgcccga
     3421 cttcttcatg gacgaggcga tcccgctggc ggacatagtc tgcccgaatg tgttcgaggc
     3481 cgagctactg tcgggtatcg tgatagaatc cgacgacgac ctggcgcggg ccaccgacgc
     3541 gatccacgcc aaggggccgg gcgtggtgat ggtgaccagc tataggccgc catcaagaga
     3601 ccgcatcggc ctgtacgtga gcgacggccg gacgaaactg gccgcgtccg cgcccatcat
     3661 ccgcttcgat aagcctccca aagggtcggg agacctgtcg agcgccctgt tcctcggtta
     3721 ctacctgatt gggggcgggg taggtgaggc gatcgagaaa acgctcgacg cgctgtactc
     3781 ggtgctcgag tcgagcgcct cgggcggcgg cgacaacctg gcgattgtcg ccgcgcagga
     3841 tgctatagcc gaaccggggc ggcgcttcac ggcgaccgcc ccgaccggct aggccgcgga
     3901 gcttacttct tgcgggggat gaccggcgcg aaggtccgcg ggtcaggcaa ggcgaaaccg
     3961 gtgggcatct cactggcgct ggcgagcagc caggtctggt tcctgccgag cagtccgcca
     4021 gtgcccttga gcgagccgcg taccgtcagc ttcgagcctt cgcgcttgat ggagcaatca
     4081 tacaccttgc cgtcggctgg gtcgatgatc gagcctttcc attccttgcc cttgtcctgc
     4141 aactcgtaga cgaaatccag gccgcagttc ggcgggtctc cggctagcgc gtccgcccga
     4201 aatttcttcg tggccagcgt atcgtcgatg agcccggtat cgaggctgat ggtggctatc
     4261 atgcggccgt acagcttgcc gccgttcatg tagagcatga cgtaggcgtt cggcttgccg
     4321 gtcttgtcgt cgatgatttt ccataggcc
//
LOCUS       DAOPHV010000177         4337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_27453_length_4337_cov_4.184960, whole genome shotgun sequence.
ACCESSION   DAOPHV010000177 DAOPHV010000000
VERSION     DAOPHV010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27453_length_4337_cov_4.184960"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLQ29_05940"
     CDS             complement(<1..248)
                     /locus_tag="PLQ29_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86225.1"
                     /translation="MRNTIIVALAALVSISAAYSQEADEGTIDDREPALSVSVGPEVA
                     ASLTGAFAGVQAQFAWRPGFLGLVAGVRADVYLSQPDV"
     gene            385..591
                     /locus_tag="PLQ29_05945"
     CDS             385..591
                     /locus_tag="PLQ29_05945"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HPG86226.1"
                     /translation="MDQDRIEAIETKLAYLEGSVSDIDALVYEYGRRTERLEEAVREL
                     AKRLGELGDDKAATPPASERPPHY"
     gene            complement(680..2014)
                     /locus_tag="PLQ29_05950"
     CDS             complement(680..2014)
                     /locus_tag="PLQ29_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HPG86227.1"
                     /translation="MTKDFKKRFDAVAARALGELLPDERGAISYSAELSAFMRFNGGK
                     VRQSGSVEQADLSIKLWKGAKTYSFMLALSGDEGEDDERVAEAVGRARALMPLLPDDP
                     YQAAPEAAGSSDARYSGTLLPEAEIPARVLGPAAGLDFTGIYSQGLVCRGAANSAGAR
                     HWFQTETFLVDYSAWLPNGRAVKSSYAGLEWSDAEYAKRIAATKRSLENLGVEQIALE
                     PGKYRAFITADALAEVVTFFSWNGFGERGLRQGESAYIALREGRESMSPRFGFSQDFS
                     LGVEPAFNDDGELAPERLEIVSGGAIANTVVCSRTAKQYGVPANGAGEDESVRSISMD
                     AGDLAEADALKAIGTGVYVSNFHYLNWSDVQAARVTGMTRFACLWVENGEIVGPIKDM
                     RWDESLYNMLGAKLEAVTKERHLLVASETYERRQVGGSLLPGILVDGLSFTL"
     gene            complement(2011..3456)
                     /locus_tag="PLQ29_05955"
     CDS             complement(2011..3456)
                     /locus_tag="PLQ29_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPG86228.1"
                     /translation="MQNDIRRLLDAAASAAKEAAWVGLRRRRTSASYYMARDGAFDQA
                     IGGLDDGVMVEVLYDGQFAYGATPDLSPEGVARAAASALVAAKAAAGKGVHRFDASVR
                     PATRVSYRTPRARKAPIAPDGVFSFLIDVCDALKAGDEIIQTVSMVEIGRYDYEIVST
                     NGADVEQSLHYMGTSLQAVARRGDVVQLRTANGPRGLTRQGGWELFDAAGLMADARRV
                     GAEAIELLGAETCPSDRRTLVLTPDQMMLQIHESVGHPLEIDRILGDERNYAGGSFVK
                     LSDIGGFQYGSKLMNISYDPTVPGELASYGADEIGNLAEKRLLIENGTLVRALGSLES
                     QARSGVPGVANQRASSWNRPPIDRMANLNLEPGETSLDSMIASIEKGVLMRTNRSWSI
                     DDYRNKFQFGCEYGTLIEDGKLTRTVRDPNYRGVSSTFWRNLCAVGDASTFAAFGTPN
                     CGKGEPNQVMFVGHASPVCAFADVEVFGGGK"
     gene            3575..>4337
                     /locus_tag="PLQ29_05960"
     CDS             3575..>4337
                     /locus_tag="PLQ29_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG86229.1"
                     /translation="MRVVIIGAGMIGLHIARELIDEGRDVVLVEKDAEAARRAGDELD
                     CLVVNEDGSRPETLRKAGVSGSAWFLALTGSDEVNIVACGLVAAECPDVHTVARVENP
                     FYSSLSAAQRRAFGLSVLINPAAETAAAIGRIVDEGFAEDLIPLHDGRLHLRTVLAGS
                     APAFVGRPLRDAKAEAGNGVLVAAVVRDKGIVVPDGNFIVEATDLLYLLGEPATLDGF
                     LGQVAGVKAGSKRVMVIGATAIGERLVERFVQEAKP"
BASE COUNT          678 a         1413 c         1521 g          725 t
ORIGIN      
        1 taaacgtccg gctggctcag gtacacgtcc gcacggaccc ccgcgacgag gcctaagaag
       61 ccgggccgcc aggcgaactg ggcctggacg cccgcgaagg cgccggtcag gctggccgcg
      121 acctctgggc cgaccgagac ggacagagcc ggctcgcgat cgtctatggt tccttcgtcc
      181 gcctcttgcg agtaggccgc cgagatggat acgagcgccg ccagcgctac gatgatagtg
      241 tttctcatga tgctgctcct ggcgccggtt tgctatgctc gcctcaatgg cgcggtccgc
      301 ggcgcggacg gacgcgatgc cgataccgta tactatatcg cgccgagtta gaagcgacga
      361 agcctcggcg gtcggaggat gacgatggac caggatagga tagaggccat agagacgaag
      421 ctcgcgtacc tcgaaggctc ggtaagcgat atagacgcgc tcgtctacga atacggccgg
      481 cgtaccgaga ggctcgagga ggcggtgcgc gagctggcca aacgccttgg cgagctgggc
      541 gacgataaag ccgcaacgcc gccggcctct gaacggccgc cacattactg agccgagccc
      601 cagggtaaga accgcccctc gtccaggggg acgaggggct attaaaagga ggcggccggg
      661 agggcggccg cctccgtcct tacagcgtga acgacagccc gtcgaccagg atgccgggca
      721 ggaggctgcc gccgacctgc ctgcgttcgt aggtctccga ggccacgagc aggtggcgct
      781 ccttggtcac ggcctcgagc ttggcgccga gcatgttgta cagagactcg tcccagcgca
      841 tgtccttgat cgggccgacg atctcgccgt tctccaccca gagacaggcg aagcgggtca
      901 tgccggtgac gcgagccgcc tgcacatcgc tccaattcag gtagtggaag ttcgacacgt
      961 agacgccggt gccgatggcc ttgagcgcgt cggcctcggc caggtcgccc gcgtccatcg
     1021 agatcgaacg tacgctctcg tcctcgccgg cgccgttggc cgggacgccg tactgcttgg
     1081 ctgtcctcga gcagacgacc gtgttggcta tcgcgccgcc ggagacgatc tcgagccgct
     1141 cgggagccag ctcgccgtcg tcgttgaagg ccggctccac gccgagcgag aagtcctggc
     1201 tgaagccgaa tctcggcgac atgctctcgc ggccctcgcg cagcgcgatg taggcgctct
     1261 ctccctgcct gagaccgcgc tcgccgaagc cgttccacga gaagaacgtg actacctcgg
     1321 cgagggcgtc cgcggtgatg aaggcgcggt acttgcccgg ctcgagcgca atctgctcga
     1381 cgccgaggtt ttcgagggag cgcttggtag ccgctatgcg cttggcgtac tctgcgtcgc
     1441 tccactcgag gccggcgtag gatgacttga cggcgcggcc gttcggcagc caggccgagt
     1501 agtcgacgag gaaggtctcg gtctggaacc agtgcctggc gccggcggag ttggccgcgc
     1561 ctcggcagac gaggccctgc gagtagatgc ccgtgaagtc gaggccggcc gccgggccga
     1621 gcacgcgggc gggtatctcc gcctcgggca atagcgtgcc tgagtagcgc gcatcggacg
     1681 agccggccgc ctcgggcgcg gcctggtagg ggtcgtccgg tagcagcggc atcagcgcgc
     1741 gcgcccgacc gacggcctcc gcgacgcgct cgtcgtcctc gccctcgtcg cccgaaaggg
     1801 ccagcatgaa cgagtaggtc ttggcgccct tccagagctt gatcgacagg tcggcctgct
     1861 cgaccgagcc tgactgccgg accttgccgc cgttgaagcg catgaaggcg ctcagctcgg
     1921 cggagtagct gatggctccg cgctcgtccg gtagcagctc gccgagggcc cgggcggcca
     1981 cggcgtcgaa tcgtttcttg aagtccttgg tcattttccg cctccgaata cctcgacgtc
     2041 ggcgaacgcg cagaccgggc tggcgtggcc gacgaacatc acctggttcg gctcgccctt
     2101 gccgcagttc ggggtgccga acgcggcgaa ggtgctcgcg tcgccgacgg cgcacaggtt
     2161 gcgccagaac gtgctcgaga cgccgcggta gttcgggtcg cggacggtcc tggtcaactt
     2221 gccgtcctcg atcagcgtgc cgtactcgca accgaactgg aatttgttgc ggtagtcgtc
     2281 gattgaccag gagcggttgg tgcgcatcag cacgcccttc tcgatggacg cgatcatgga
     2341 atcgagcgag gtctcgccgg gctccaggtt caggttggcc atgcggtcga tcggcggccg
     2401 gttccacgag ctggcgcgct ggttggcgac gccggggacg ccggagcggg cctggctctc
     2461 gaggctgccg agggcgcgca cgagcgtgcc gttctcgatg agcagccgct tctcggccag
     2521 gttgccgatc tcgtcggctc cgtaggaggc cagctcgccc ggcacggtcg ggtcgtagct
     2581 gatgttcatg agcttggagc cgtactggaa gccgcctatg tcggacagct tgacgaagct
     2641 accgccggcg tagttccgct cgtcgcccag gatacggtct atctcgaggg gatggccgac
     2701 gctctcgtgg atctggagca tcatctggtc gggcgtcagc accagggtgc ggcggtcgct
     2761 ggggcaggtt tccgcgccga gcagctcgat ggcctcggcg cccacgcggc gggcgtcggc
     2821 catcagtccg gccgcgtcga acagctccca gccgccctgc ctcgtcaggc cgcgcgggcc
     2881 gttcgcggtg cgcagctgga ccacgtcgcc ccgcctggct acggcctgga ggctcgtgcc
     2941 catgtagtgc agagactgct cgacgtcggc gccgttggtc gagacgatct cgtagtcata
     3001 gcggccgatc tcgaccatcg acacggtctg gatgatttcg tctccggcct tgagggcatc
     3061 gcagacgtcg atcaggaacg agaatacacc gtccggggcg atcggggcct tacgggcccg
     3121 aggcgtccgg tacgatacgc gcgtcgcggg gcggacggac gcgtcgaaac ggtggacgcc
     3181 cttgccggcc gccgccttgg cggcgacgag agccgacgcg gccgcccgag ccacgccctc
     3241 tggcgacagg tcgggggtgg cgccgtaggc gaactggccg tcgtagagta cctcgaccat
     3301 gacgccgtcg tcgaggccgc ctatggcctg gtcgaaggct ccatcacggg ccatgtaata
     3361 tgaggcgctc gtgcgccgcc gcctgagccc gacccatgcg gcttccttgg cggccgaggc
     3421 ggccgcgtcc aggagccggc gaatatcgtt ctgcatggct ctctccttta ttgcgatctg
     3481 cgttgcgtca gcggcgacga gtgtaacgtt caaataagtt gaacgtcaag gtgaaagctt
     3541 atctaaaatc taatttgggc gtatagtggc cgcgatgcga gttgtaataa tcggggccgg
     3601 gatgatcggc ctccatatag cgcgggagct catagacgag ggtcgggacg tcgtactcgt
     3661 cgagaaggac gccgaggcgg ccaggcgggc cggcgacgaa ctcgattgcc tcgtggtgaa
     3721 cgaggatggc agccgtcccg agaccctgcg caaggctgga gtctcgggct cggcgtggtt
     3781 cctggccctg accggcagcg acgaggtgaa catcgtcgcc tgcgggctcg tggcggccga
     3841 gtgccccgac gtccatacgg tggccagggt ggaaaacccg ttctattcgt cgctctcggc
     3901 cgcccagcgc cgagcgttcg gccttagcgt gctgatcaac ccggcggccg agaccgccgc
     3961 cgccataggc cgcatcgtcg acgagggctt cgccgaggac ctgataccgc tccacgacgg
     4021 tcgcctgcat ctgcgcacgg tcttggccgg ctcggcgccg gctttcgtcg gcaggccgct
     4081 ccgcgacgcg aaggccgagg cggggaacgg cgtgctcgtg gctgccgtgg tccgcgacaa
     4141 gggcatcgtc gtgcccgacg gcaacttcat cgtcgaggcg accgacctcc tgtacctgct
     4201 cggcgagccc gccaccctcg acggcttcct cggccaggtg gccggggtca aggccggttc
     4261 caagcgcgtc atggtcatcg gcgccacggc tatcggcgag cggctcgtcg agcgcttcgt
     4321 ccaggaggcg aagcccg
//
LOCUS       DAOPHV010000178         4334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_27475_length_4334_cov_4.397289, whole genome shotgun sequence.
ACCESSION   DAOPHV010000178 DAOPHV010000000
VERSION     DAOPHV010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27475_length_4334_cov_4.397289"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PLQ29_05965"
     CDS             complement(<1..453)
                     /locus_tag="PLQ29_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HPG86230.1"
                     /translation="MRKKYGQNFLVSGRARSRIASLFETEPGARAWEIGPGAGAMTRE
                     ALLLGLSLSAFEIDKGFAEFIRGAYGAMPGFRLYEGDFVKTWRAAFETDGRPSLAFGN
                     LPYNAAGAIIAALIEGGVRPPRMVFTVQKEAAQRMCAAPSTMNYSAFSV"
     gene            complement(578..1993)
                     /locus_tag="PLQ29_05970"
                     /pseudo
     CDS             complement(578..1993)
                     /locus_tag="PLQ29_05970"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
     gene            complement(2226..3413)
                     /locus_tag="PLQ29_05975"
     CDS             complement(2226..3413)
                     /locus_tag="PLQ29_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86231.1"
                     /translation="MHRIRVAIAFMLAVALTGASAQESSELIDTYRRNFVRSSLGTKL
                     ELLKEASAYDGVDMGPLYDTAIQFVLSNASLLSTDALLRDISVLSVNMVRKYKYAPAA
                     GHLWELFGIYKDALVRVPLLQTLAEVAVGNPAILKELNAFVDTQVSLAKSGVRIDQAV
                     LDAAIYSLGRLGDSSSFPFLFAAYTASIGKAVTDRAGAAMASLKGDYAAFLAEVIRSS
                     PPADKAAALEAGLRGGALPEERRGELAEVALSVGVSYQSPSSGDQGYILGMRTQAARE
                     LTARSWQKASPLAIKHFYDFQVQYNRGQVSKSNFLESIALLGAMGTAEAAQALALYLQ
                     LINTETEQGKSFDEQVTLAVVNNLGRLGDKAAFDYLLYIGYLQYPETVKKAARDALQK
                     LRW"
     gene            complement(3434..>4334)
                     /locus_tag="PLQ29_05980"
     CDS             complement(3434..>4334)
                     /locus_tag="PLQ29_05980"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPG86232.1"
                     /translation="IMEMCRIAGVPDSTAALWSIRELDACIKAFAKKYRAEGMERDRG
                     AIVFMEKLYEFRKRLEFELPKYRAGITSSRFIRANQRIRVLVEGIGVYSSTVIDSNER
                     YLVVSYPVGARVPPGFQWKGARVSVYFWRQEDAGYVFDTYVLEDLRIRDIPVLQLGHS
                     GSLLRTQKRKSLRVRSRMPAYLYLLKRLEGAYEKPERVPGLKCVVQDVSEDGVSVLIG
                     GKARIGLLVKAQFYIGDDQIVISGTVKGSEYDVEKNQSLLHVEAMTPSPRMRNVIRSH
                     VYNVGPGDSERRGAELDERPFSV"
BASE COUNT          720 a         1460 c         1577 g          577 t
ORIGIN      
        1 caccgagaag gccgagtagt tcatggtcga gggggcggcg cacatgcgct gggcggcctc
       61 tttctgcacg gtgaagacca tgcgcggggg gcggacgccg ccctcgatca gggccgcgat
      121 gatcgccccg gccgcgttat acggcagatt gccgaacgcg aggctcggac gcccgtccgt
      181 ttcgaaggcc gcgcgccagg tcttcacgaa gtcgccctcg tagagccgga agccgggcat
      241 ggcgccgtag gctccgcgga tgaactcggc gaagccctta tcgatctcga acgcgctcag
      301 gctcaggccg agcagcagcg cctcccgggt catggcgccg gcgcccgggc ctatctccca
      361 ggcgcgcgcg cccggctcgg tctcgaacag cgaggctatg cggctcctgg cgcgacccga
      421 gaccaggaaa ttctgtccgt acttcttgcg catgccgagg ccgaggccgt cgagcatggc
      481 tgacagctcc gagggggcgt cgtagttgac tggaagcggc acggcgccgg gattcgatgc
      541 ggtaggcgac atggggccaa gcatacacga acgcggcggc gcaggcaacg acgccggcca
      601 tggcggcgcg ccagccagtc caatcctcgg acagggctat cgagggcagg cgggcggcgg
      661 tcccgatgac ggcgacgagg gcccggtacg gcagcaggca ggcgagccgg gcggccggcg
      721 cggcccaggg caccagggcg acgacgaacg agccgacgag gccggcccag atcagcgccg
      781 ccacgagcag cccggccaac gcgctcgcga ccggcgagaa gaggttgagc gttccgaagg
      841 ccagtagcga cagcggggcc gtggccgcga gcgccgcgaa gcccacggct accgctcccg
      901 ccgccggagg cggcagccag cgccggaggc cgaactcgta ggccccgccg agaactccga
      961 tgccggcgac cgccaggtac gacagcccga acgacagctc gtgggccgcc gcgggatcga
     1021 cgacgacggc gacgatgaac accgccgaca gcgtgacgac cgccggctgc gggcggtcgg
     1081 ccgccaggag ggcgcttgta gcccagaaca tggcgaccgc ccgcgctacc gacggcgagg
     1141 cgcccacgag gtacaggtag gccgccgcga gcaggcaggc taccgcgcgg gcgcgcctcg
     1201 ggccgagcgc gaagccgagc aggaccgcca cgaacgaagc caggatcccg acgtgctggc
     1261 ccgacagcgc gagtatatgg gcgcagccgg cggccctgaa accggccgag agctccgggt
     1321 cgagctcgtc gcgcaccccg acgacgaggg cctcgaggag ggggccggcc tctccgccgg
     1381 ccgcccggag cgactcgagg gcgccggccc ggagcgcggc ccgagcggcc tcggcagcgg
     1441 gcggcggccc gatagcctcg acgccggccg cgtcgacgaa ggcgtacggc gaggcgagcg
     1501 cgtcgcgcca ggagccgccg ccggtcctcg gcggcaggag gccgcgcctt agggcgacgc
     1561 gcaggcgctg gccgcgcacg ggcgcctcgc cagggcggcc gtcccgaggc gcaccgccga
     1621 ttgacgagcc gcggacgtag acgacgacgc gacccgaggc ggtgacgcgt tctccgtcgc
     1681 ggccgagcgc cgcctccagc tccagctcgc aggcgaggag gcctccggcc gtatgggtcg
     1741 ggtcggaggc gacccgagct tcgtaggcgg ccgcgccggg ggcgtacggc ccgccgagcc
     1801 ccggactgaa acgcgccgag ccggcctcga cgcgggcatg gcaccaggcc ccgcagcaca
     1861 gcccggcggc cagagccgcc gcgagcgccg acgccgctcg ccggaggcca cctatcgccg
     1921 gctcggcgac ggcgcgcagg cgccgtttcg taaccaggga tatcgtcgac ctggggcttg
     1981 gagcgaaacg catcgagagc gcgatggaag ccgcgcacgc ggcgatgacg ctggccaggg
     2041 ccctcgaacc ggaaccggga ggcaggtaga gcgcggcggc cagcccggcc gccgccgcga
     2101 cgaccggcgg cacggagacg cgcggaggct cgacggagga cgcgcgcgtt ttgacggatt
     2161 gagcggccac gcccggatac gcggctcgga gcctcggcgc gtgcgctatc caggaaggcc
     2221 gagcgttacc agcgaagctt ctggagcgcg tccctggcgg ccttcttgac cgtctcggga
     2281 tactggaggt agccgatgta caggagatag tcgaaagccg ccttgtcgcc gaggcggccg
     2341 agattgttga cgaccgcgag cgtgacctgc tcgtcgaagg acttaccctg ctccgtctcg
     2401 gtgttgatca gctggaggta gagcgccagg gcctgggcgg cttcagcggt gcccatcgct
     2461 ccgagcagcg cgatcgactc gaggaagttc gacttggaga cctggccgcg gttgtattgc
     2521 acctggaaat cgtagaagtg tttgatagcg agcggcgagg ccttctgcca cgatcgggcg
     2581 gtcagctccc tggcggcctg ggtacgcata cccaggatat agccctggtc gcccgacgac
     2641 gggctctggt acgagacgcc gaccgacaac gccacctcgg ccagctcgcc ccggcgctcc
     2701 tcgggcaggg cgccgccgcg caggccggcc tcgagggccg cggccttgtc ggcgggcggg
     2761 ctcgatcgga tgacctcggc gaggaaggcc gcgtagtcgc ccttgagcga cgccatcgcg
     2821 gcgccggccc gatcggtgac ggccttgccg atggacgcgg tataggccgc gaagaggaag
     2881 gggaacgacg acgaatcgcc gaggcggccg agcgagtaga tggcggcatc gagcacggcc
     2941 tggtcgatcc ggacgcccga cttggcgagc gagacctgag tgtcgacgaa ggcgttgagc
     3001 tccttgagga tggcggggtt gcctacggcg acctcggcca gcgtctgcag gagcggcacg
     3061 cggacgaggg cgtccttgta gataccgaat agctcccaga ggtggccggc ggccggcgcg
     3121 tacttgtact tacgcaccat gttgaccgat agcaccgata tgtcgcgcag gagcgcatcg
     3181 gtggacagga gcgaggcgtt gctgagcacg aactggatgg ccgtgtcata caggggcccc
     3241 atgtcgacgc cgtcgtaggc cgaggcctcc ttgagcagct ccagcttggt gccgaggctc
     3301 gagcgcacga aattcctgcg gtaggtgtct atgagctcgg acgattcctg ggccgacgcg
     3361 ccggtgagcg cgacggccag catgaacgct atcgctacgc ggatcctatg catcgttccc
     3421 ccgtccgtta aacttatacc gaaaacggcc tctcgtccag ttcggcgccc cggcgctccg
     3481 aatcgcccgg accgacgttg tagacgtggg atcggatgac gttgcgcata cgcgggctcg
     3541 gcgtcatcgc ctcgacgtgc aatagggatt gattcttctc gacgtcgtac tccgagcctt
     3601 tgacggtgcc gctgatgacg atctggtcgt cgcctatgta gaactgcgcc ttgacgagca
     3661 acccgatcct ggccttgccg cctatcagca ccgatacgcc gtcctccgac acgtcctgca
     3721 ccacgcactt gaggccgggc actcgctccg gcttctcgta ggcgccctcg aggcgcttga
     3781 gcaggtacag gtaggccggc atcctggagc ggacgcgcag cgacttgcgt ttctgggtgc
     3841 gcaggagcga ccccgagtgc ccgagctgca gcaccggtat gtcgcggatg cgcagatcct
     3901 cgagcacgta ggtatcgaac acgtagccgg cgtcctcctg gcgccagaag tacaccgaca
     3961 cgcgggcgcc cttccactgg aagccgggcg gcaccctggc gccgaccggg taggagacga
     4021 ccaggtagcg ttcgttcgag tcgatcaccg tggacgagta gacgccgata ccctcgacga
     4081 gcacgcggat gcgctggttc gcccgtatga agcgggaact ggtgatgccg gcgcggtact
     4141 tgggcagctc gaactcgagc cgcttcctga actcgtacag cttctccatg aagacgatgg
     4201 cgccgcggtc gcgctccatg ccttcggcgc ggtacttctt ggcgaaggcc ttgatgcagg
     4261 cgtcgagctc gcgtatcgac cagagggccg ccgtcgaatc gggcacgccg gctatcctgc
     4321 acatctccat gatg
//
LOCUS       DAOPHV010000179         4299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_27800_length_4299_cov_3.822573, whole genome shotgun sequence.
ACCESSION   DAOPHV010000179 DAOPHV010000000
VERSION     DAOPHV010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27800_length_4299_cov_3.822573"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..493
                     /locus_tag="PLQ29_05985"
     CDS             287..493
                     /locus_tag="PLQ29_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86233.1"
                     /translation="MKDLRLSSQGGEYVAAPLKAWPTADVESLAATVESDYDLVIVDL
                     FYHDADGVAMALSQADLDTIRHKA"
     gene            complement(711..1277)
                     /gene="efp"
                     /locus_tag="PLQ29_05990"
     CDS             complement(711..1277)
                     /gene="efp"
                     /locus_tag="PLQ29_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPG86234.1"
                     /translation="MLMTSDLRVGTVFKSGKDVVVVQRMLGNKGGRGGVVVKMRVKNL
                     LTGSTSDLGLDSGEKFEEVMLDQHKMRLSFIDGDNYVFMDQESFEQYELTKDDLGDNA
                     QYITPEDDFDVEITFYEGKPVGVVLPISIERTITYCEPGIKGDTSGKSSKPATLDSGC
                     VVQVPLFCDIGTRIRLDTRDGSFQERVK"
     gene            complement(1389..2597)
                     /gene="earP"
                     /locus_tag="PLQ29_05995"
     CDS             complement(1389..2597)
                     /gene="earP"
                     /locus_tag="PLQ29_05995"
                     /inference="COORDINATES: protein motif:HMM:NF021598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HPG86235.1"
                     /translation="MTIDALCRVVDNYGDIGVVYRLARALSALPDPPRLRLVVDDLRA
                     FSLLEPAVDPALPVQTLRGWTVLDWAGPDAAGSPPGSGARAYAEAYADGPPLVVLECF
                     ACGRPDWFEAILFDDGRYLDGAGAAPTRTIVNLEYLSAEAYPDEFHRLPSLTRSSSVR
                     KHMFMPGFTPATGGLIQDREFAESLRLAGELGSKRALRSAISDGLGRAVDPDSFWALL
                     FGYERDYGPIVEDLAARAQASPIVAFAAAGKSRPCVEAAWERAGRPFELVGLPFMRQQ
                     DWDRLVAAADFALVRGEDSLSRAALAGIPFLWHAYPQGGDQALKVRGLLERMRPFFGP
                     DDFAPIEAAFVDVNDRKVDGPTVRGYERIGALLEPSVRVRAGFSAFSETLRKNGDLAA
                     NLMTFLREIV"
     gene            2752..>4299
                     /locus_tag="PLQ29_06000"
     CDS             2752..>4299
                     /locus_tag="PLQ29_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPG86236.1"
                     /translation="MTSQAVAKRDEARATPLDEGRNAFDELSRASALLSTADDFSEIQ
                     AVLVEQACDISRSDLAALYLYSNDGEALRLSYRRGPWQAPASLDRASETIAFVEECGE
                     AAVLNRRRDCALVGLLLDPAMDSGMAVPISLPRYRAGLLVLNARGADHYGRSRLNFLS
                     SFCALAAGMLRSAKLFKELKERAAEIEALERYQASVFSSMTNLLVTSERDGTLRFYND
                     EAAEAFGLDEGCIGKPVIELLAGRLDDSVLTAIGNSLRDGTTLLGIEGIAKGDLAKGL
                     RDDLDFSLNMSPVRGRRSDRHDGVVLTFTDQTRERRLKEQVDVVVEERRAIKDMFSRY
                     LSEDVVHSLMESPEQVKLGGAGKRATVFFADIRGYTSFSEGKTPEYIIGILNEYFSEA
                     VEVVLRYRGFIDKFIGDCIMAAWGVPMTSEEDDARNAVACAIDIQGLISNPKRTFFRG
                     EAEHLRVGIGVHTGYLVAGNLGSARRMNYSVIGDTVNIAARLEGVAKAGEVIITESTK
                     ELLGGQFR"
BASE COUNT          792 a         1397 c         1423 g          687 t
ORIGIN      
        1 gagaagcgtg tctaccagcc cgcacgaggc cgcggacgcg acgacgccga ggatgacgaa
       61 aactcgaacc gcgatccttc ccatactccg catagttccc ttctttccag ggtggacgga
      121 gccgatgcgg ggaagcgagc gcggccgatg ggtgtggtac gtatcccagc cgggtttcgg
      181 gctctctggc cctggtacca tgaaagcata tcgcgacgag cgaggcaatg caaacgtccc
      241 gcggctccgg gtggtctagg ccttgcagaa ctaatccaag tctctggtga aggacttgcg
      301 gctatcgtcg caggggggcg aatatgtggc cgccccgcta aaggcctggc cgacagccga
      361 cgtagaatca ctcgccgcga cagtcgagag cgactacgac ctcgtcatcg tggacctgtt
      421 ctaccatgac gccgacggcg tggcgatggc cctgagccag gccgacctcg atacgattcg
      481 ccacaaggcc tgaggaggca ggagactgct catccgctac atgagcgtcg gcgaggcgga
      541 ggactaccgg ttagccgccc gcctggaaag ccgtgctgta cggcgagcgg cgcctacctg
      601 gacataatag acgcgttcga gtacttcgag gaggcgctcg ggctctagcg cggcgaaaaa
      661 aaaccgcggc catgcggccg cggcttcgta gtagcgctcg gtagcctgcc ttacttcacg
      721 cgctcctgga agctaccgtc acgggtgtcg agcctgatcc tcgtgccgat gtcgcagaac
      781 aggggcacct ggaccacgca gccggaatcg agggtagccg gcttggacga ctttcccgag
      841 gtgtctccct tgatgccggg ctcgcagtag gtgatggtcc gctcgatgga gatcgggagc
      901 acgacgccga ccggcttgcc ctcgtagaag gtaatctcga cgtcgaagtc gtcctcgggc
      961 gtgatgtact gggcgttatc gccgaggtca tccttggtca gctcgtactg ctcgaagctc
     1021 tcctggtcca tgaagacgta gttgtcgccg tcgatgaagg agaggcgcat cttgtgctgg
     1081 tcgagcatga cctcctcgaa cttctcgcct gagtcgaggc cgaggtcgga ggtcgagccg
     1141 gtcagcaggt tcttgacccg catcttgacg acgacgccgc cgcggccgcc cttgttgccg
     1201 agcatgcgct ggacgacgac gacgtccttg ccggacttga agacggttcc cactcgtaga
     1261 tcagatgtca tcagcatagt atttccccgt gataaaagaa ttgctatcgg taggcactga
     1321 cgccgatggc gctggccgcg ccgccggata gcgctcctgg atagagcatc gcttcgaacc
     1381 gccgtattct acactatttc gcgcaagaaa gtcatcaggt tggcggccag gtccccgttc
     1441 ttcctgaggg tttcggagaa ggcggagaaa cccgcgcgta cccgcacgga cggctcgagc
     1501 agcgccccga tcctctcgta gccgcgcacc gtaggcccgt cgaccttccg gtcgttgacg
     1561 tcgacgaagg ccgcctcgat gggcgcgaag tcgtccggcc cgaagaaggg gcgcattcgc
     1621 tcgaggagcc ctcgtacctt gagcgcctgg tcgccgccct gcggatacgc gtgccatagg
     1681 aagggaatgc cagcgagggc ggctcgcgac agcgagtcct caccgcgcac gagggcgaaa
     1741 tcggcagccg cgaccaagcg gtcccagtcc tgctggcgca tgaacggcaa gccgaccagc
     1801 tcgaagggcc ggccggcgcg ctcccaggcc gcctcgacgc agggccgact cttgccggcg
     1861 gccgcgaagg ctacgatggg cgacgcttgg gcccgcgcag ccaggtcctc gacgatgggg
     1921 ccgtagtccc gctcgtagcc gaacagcagg gcccagaagc tatccgggtc cacagcgcgg
     1981 ccgaggccgt cgcttatggc cgaccggagg gcccgcttgg agccgagttc gcccgccagc
     2041 cggagggact cggcgaactc gcggtcctgg atcagacctc cggtggccgg cgtgaagccc
     2101 ggcatgaaca tgtgcttgcg taccgacgac gagcgggtca gggacggcag gcggtgaaac
     2161 tcgtcaggat aggcctcggc gctcaggtac tccaggttga cgatggtcct cgtcggcgcg
     2221 gcgccggccc cgtcgaggta gcgcccgtcg tcgaacagga tcgcctcgaa ccagtccggg
     2281 cgaccgcagg cgaaacactc gagcacgacc agcggcggcc cgtcggcgta agcctccgcg
     2341 taggctcgcg cgccggagcc cggcggagac ccggccgcgt ccggcccggc ccagtcgagc
     2401 acggtccaac cgcgcagggt ttgaaccggc agggccggat cgaccgccgg ctcgagcagg
     2461 ctgaaggccc gcaggtcgtc gaccacgagt cggagccgag gcgggtcggg caaggccgac
     2521 agggcgcggg ccaggcggta tacgacgcct atgtcgccgt aattatccac caccctgcag
     2581 agcgcgtcga tcgtcatggc gaccgagtat accgaaactc cgccggcaat tcgatacccg
     2641 catctcgata caagcttcgc gaaggctcgt cgtcggattt cagccgattc gattcgatct
     2701 atggacgaaa tggcgctaca atgtagctag aaaaaagtcg gaggcgagac gatgacgagt
     2761 caggccgtag ccaagagaga cgaggcacgg gcgacacctt tagacgaggg caggaacgcc
     2821 ttcgacgaac tatcgcgggc ctcggcgcta ctgtcgacgg ccgacgattt ctcggagatc
     2881 caggccgtgc tcgtcgaaca ggcctgcgat atcagccgct ccgacctcgc cgccctgtac
     2941 ctgtactcga acgacggcga ggcgctcagg ctgtcgtacc gtcgcggccc ctggcaggcg
     3001 ccggcgtcac tcgaccgggc ctccgagacg atagccttcg tcgaggaatg cggcgaggcg
     3061 gccgtgctca accgcaggcg ggactgcgcc ctggtaggcc tgctcctcga cccggcgatg
     3121 gactcgggca tggcggtccc gatatcgctg ccgcgatacc gagccgggct tctcgtgctc
     3181 aacgcccggg gcgccgacca ctacggccgc tcgcgcctca atttcctgtc gtcgttctgc
     3241 gccctcgccg ccggcatgct caggagcgcc aagctgttca aggagctcaa ggagcgagcc
     3301 gccgagatcg aggcgctcga gcgctaccag gcgagcgtct tctcgtcgat gaccaacctc
     3361 ctcgtcacct ccgagcgcga tgggacactg cgtttctata acgacgaggc ggccgaggcc
     3421 ttcggcctcg acgaaggctg cataggcaag ccggtgatcg agctactggc cggcaggctc
     3481 gacgacagcg tgctaaccgc catcggcaac agcctgcgcg acggcacgac gctactcggc
     3541 atcgagggta tcgccaaagg cgacctggcc aagggcctgc gcgacgacct ggacttctcg
     3601 ctcaacatgt cgccggtacg cggcaggcgc tccgaccgcc acgacggcgt cgtgctgacc
     3661 tttaccgacc agacgcggga gcgccggctc aaggagcagg tggacgtcgt cgtcgaggag
     3721 cgcagggcca tcaaggacat gttcagccgc tacctatcgg aggacgtggt ccacagcctg
     3781 atggagtcgc ccgagcaggt caagctcggc ggcgcgggca agcgggcgac cgtgttcttc
     3841 gccgacatac gcggttatac ctcgttctcg gagggcaaga cgcccgagta catcatcggg
     3901 atactcaacg agtacttctc cgaggcggtc gaggtcgtgc ttcggtaccg cggcttcatc
     3961 gacaagttca taggcgactg catcatggca gcctggggcg tgccgatgac cagcgaagag
     4021 gacgacgctc ggaacgccgt cgcctgcgcc atcgatatcc agggcctcat cagcaacccc
     4081 aagcgaacgt tcttccgcgg cgaggccgag cacctgcgcg tcggcatcgg cgtgcatacc
     4141 ggctacctgg tcgccggcaa cctcggctcg gcgcgcagga tgaactactc ggtcatcggc
     4201 gacacggtca acatcgccgc caggctcgag ggagtcgcca aggccggcga ggtcatcatc
     4261 accgaatcga ccaaggagct gctcggcggt cagttcagg
//
LOCUS       DAOPHV010000180         4291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_27861_length_4291_cov_4.056421, whole genome shotgun sequence.
ACCESSION   DAOPHV010000180 DAOPHV010000000
VERSION     DAOPHV010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27861_length_4291_cov_4.056421"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLQ29_06005"
     CDS             complement(<1..866)
                     /locus_tag="PLQ29_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPG86237.1"
                     /translation="MHRYVRPFAAIACAAGLMLGASCAKPTKVADPLSVAVFVPGIAS
                     GSPIYEMLVTGATRAVDETPGARLKVIEAGYDQSTWLDSLRDAAASGEFSVIVSSNPS
                     LPELCAKLAPDYPGLRFILADAYLAGNPAIHTVLYNQKEQGYIVGVLAGLVSKDRAGP
                     GALRAALIAAQRYPTLDRLIRPGFEAGLKAVDPAFELEFREIGNWYDSAKAAELAGSL
                     YGSGVEVILPIAGSAGQGVVAAARERGKAAVWFDGGGYALDPVAVVGCATIAQDRLVY
                     ERLKLALSGDES"
     gene            1014..1478
                     /locus_tag="PLQ29_06010"
     CDS             1014..1478
                     /locus_tag="PLQ29_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPG86238.1"
                     /translation="MSPERSYKIVELTRMFGLTARTLRYYEELGLLESEDRDATEHRR
                     YSERNVVRLKRVQQLKDYGLTLSEIRELFDLAKRDRTGVSVRESLAAKYRARLEDAKR
                     RRESLDAYIEDLSWHVEQLERVSDFFDCPGASCAACRWAERCDVRALIAKDA"
     gene            1481..2938
                     /locus_tag="PLQ29_06015"
     CDS             1481..2938
                     /locus_tag="PLQ29_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPG86239.1"
                     /translation="MYCGIDVGTSSVKCLITDASGSRLGFGKAPVGLHLGDRGFEADP
                     MEWLRATAIAYAEAATGIGEATLAAGLAAVAVSGNGPTLVTVGGDGLPIGRAASWLER
                     GAVAEAERVSALAGRRVDPSFYLPKALRAIESPQGDDVAMFFSGPEYLAFALGAAPVS
                     YLADDYYDAFVWDRAVAARLGLADSLFPLYVRPATVIGSVSRQAAEAVGLPAGLPIVA
                     GFPDFLAALVGSGAVETGLACDRSGSSEALNVCADRPFPDRAIFSLPHAVSGLWNLSG
                     GLSTSGTAVEWFARASGYSTGGVSGYDGIEALLLDAERSNGDVPIFLPYLSGERAPLW
                     EPGLRGAFIGLSLDHGRKEMARAVVESIAFGLRLAADRIRGGGFDIRLARCSGGAARD
                     ASLCAIKADVLGLPVEVPASPECEAMGDACACAVALGERSSLAEASASMVAVGARYEP
                     DPRRAAAYDERYERWERALDAVLSMSAERAPKEKR"
     gene            2940..3893
                     /locus_tag="PLQ29_06020"
     CDS             2940..3893
                     /locus_tag="PLQ29_06020"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPG86240.1"
                     /translation="MIVLPPSMMQSLILFLLICVGFAAVKTRTLSPDNVRALSRFLVD
                     YTLPAVIIISMQRPFSPALRDQALRILGLSLLFYAASFPLAYLATAAYRKSGRAELGV
                     HRFAMCFSNVAFMGFPVAESLLGKESLFILSIYNIPFQVLAFSVGIVMISGRRSGAGA
                     PGTRRPSLGSLRSLVNPSIVSAVAGFALFLGSVRIPEPLYSAMDLLGGMTTPLAMVVI
                     GATLANTRIGGVLSNPRLWLTTAYRLALLPALAFAAAKAAGLTGLELSIPVLVAAMPV
                     AANSTILAGVYGGDDVTASALVFASTLLSLFTIPALGYLIA"
     gene            3976..>4291
                     /locus_tag="PLQ29_06025"
     CDS             3976..>4291
                     /locus_tag="PLQ29_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPG86241.1"
                     /translation="MKTNHPAGRRIGLKRFLSYLSPYWGLFLLGFAAMALSTLAKLAG
                     PLILRALVDESIPNKDLVGMIRYALLYLGIIVAMGALTYYQTTTIVKLGLNVVTNVKR
                     DLF"
BASE COUNT          656 a         1520 c         1368 g          747 t
ORIGIN      
        1 atcgactcgt cgcccgacag ggccaacttg agccgctcgt agaccaggcg gtcctgggct
       61 atggtggcgc agccgacgac cgctaccgga tcgagggcat agccgccgcc atcgaaccat
      121 acggccgcct taccgcgctc cctggcggcc gcgacgacgc cctgcccggc cgagccggct
      181 atcggtagga tcacctcgac gcccgagcca tagagcgagc cggccagctc ggcggccttg
      241 gccgagtcgt accagttgcc tatctcccgg aactccagct cgaaggccgg gtcgacggcc
      301 ttgagcccgg cctcgaagcc gggccggatc agccggtcga gggtcggata gcgctgcgcg
      361 gcgatgaggg cggcgcgcag cgcgcccggt ccggccctat ccttggacac gaggccggcc
      421 agcacgccta cgatatagcc ctgctccttc tggttgtaga gcacggtatg gatggccggg
      481 ttgcccgcca ggtaggcgtc cgccagaatg aagcgaagcc ccgggtaatc gggagcgagc
      541 ttcgcgcaca gttccggtag cgacgggttc gaactgacta tgacggagaa ttcgcccgag
      601 gcggccgcgt cgcgcagact gtccagccag gtcgactggt cgtagccggc ttcgatgacc
      661 ttgagcctgg cgccgggcgt ctcgtcaacg gctcgcgtcg cgccggtcac gagcatctcg
      721 tagatggggc taccggaggc gatgcctgga acgaacacgg cgaccgacag cggatcggct
      781 accttcgtag gcttcgcgca cgaggctccg agcatcaggc ccgcggcgca ggcgatggcg
      841 gcaaacggac gaacgtatcg gtgcataatc gaactcctgt cttggcgcct ccatcattct
      901 ggctcgaggc gcgctattca cgaaactcgg ccatgagtat acttatcgcg gtgctagtcg
      961 cggagtagcc gcaggcttcc gttcttgaaa gcttacgtta aaggtcaacc tgaatgagcc
     1021 ccgagcgatc gtataaaata gtagaactaa cgcgcatgtt cggcctgacg gccaggacgt
     1081 tgcgctacta cgaggagctc ggcctcctcg agtccgagga ccgggacgcg accgaacatc
     1141 gccgctactc ggaacgtaac gtcgtgcgcc tgaagcgcgt gcagcagctc aaggactacg
     1201 gcctgacgct gtcggagata cgcgagctgt tcgacctggc caagcgcgat cgcaccggcg
     1261 tatccgtcag ggagagcctc gcggccaagt accgcgcaag gctcgaggac gccaagcgac
     1321 gccgcgagtc gctcgacgcc tatatcgagg acctgtcctg gcacgtcgag cagctcgagc
     1381 gcgtgagcga cttcttcgat tgccccggcg cgtcctgcgc cgcctgccgc tgggccgagc
     1441 gctgcgacgt acgcgccctg atagcgaagg acgcctaacc gtgtactgcg gcatcgacgt
     1501 cggcacatcg tcggtcaagt gcctcatcac cgacgcgagc ggctccaggc taggcttcgg
     1561 taaggccccg gtcggcctgc acttaggcga caggggcttc gaagccgacc cgatggagtg
     1621 gttgcgcgcg accgccatcg cctacgccga ggccgcgaca ggcattggcg aagccacgct
     1681 cgcggcgggg ctcgccgcgg tagccgtatc gggtaatggc ccgaccctgg tgaccgtcgg
     1741 cggagacgga ctgccgatcg ggcgcgccgc ctcgtggctg gagcgaggcg ccgtcgccga
     1801 ggccgagcga gtctcggccc tggccggccg ccgcgtcgac ccgtcgttct acctgcccaa
     1861 ggccctgcgc gccatcgaat cgccccaagg cgacgacgta gccatgttct tctccggccc
     1921 ggaatacctg gccttcgcgc tcggcgctgc ccctgtatcc tatctcgcgg acgactacta
     1981 tgacgccttc gtctgggaca gggccgtcgc ggctcggctc gggctagccg actcgctctt
     2041 tcccctctac gtacgccccg cgaccgtcat cggctcggtc agccggcagg ccgccgaggc
     2101 ggtcggccta cccgccggcc tgcctatcgt cgccggattc cctgacttcc tcgccgctct
     2161 cgtcggctcc ggagcggtcg agacgggcct cgcctgcgac cgttccggct cgagcgaggc
     2221 gctcaacgtc tgcgccgatc gcccgttccc ggaccgcgcc atcttcagcc tgccccacgc
     2281 ggtgagcggc ctctggaacc tatcgggcgg cctgtcgacg agcggcacgg cagtcgagtg
     2341 gttcgcccgc gcctccggct actcgacggg aggagtatcc ggctacgatg gcatcgaagc
     2401 actgttactg gacgcggagc gctccaacgg ggacgtgccg atattcctcc catatctatc
     2461 cggcgagcga gcgccgctct gggagccggg tctgcgaggc gccttcatcg gcctgtccct
     2521 ggaccacggc aggaaggaga tggctcgcgc cgtggtcgag tcgatcgcct tcggcctgcg
     2581 cctcgcggcc gatcgcatac gcggcggcgg cttcgacatc cgcttggcgc gctgctcagg
     2641 cggggccgcc cgcgacgcct ccctctgcgc catcaaggcc gacgtcctgg gcctgcccgt
     2701 cgaagtgccg gcgtcgccgg agtgcgaggc catgggcgac gcctgcgcct gcgcggtcgc
     2761 cctcggcgaa cgatccagcc tggccgaggc gtcggcctcc atggtcgccg tcggggcccg
     2821 ctacgagccg gatccgcgac gcgcagccgc ctacgacgaa cgctacgaac gttgggaacg
     2881 ggccctcgac gccgtactct cgatgagcgc cgaacgggcg ccgaaggaga agcgctagca
     2941 tgatcgtcct gcctccctcg atgatgcaga gcctcatcct attcctgctc atctgcgtcg
     3001 gcttcgctgc cgtcaagacc cgtaccctgt ctcccgacaa cgtgcgcgcc ctgtcgcgct
     3061 tcctcgtgga ctacaccctg ccggccgtca tcatcatatc catgcagcgg cccttcagcc
     3121 ccgcgctgcg cgaccaggcc ctgcgcatac tgggcctctc gctgttgttc tacgcggcgt
     3181 ccttcccgct cgcctacctt gcaaccgcgg cgtaccgcaa gtccgggcga gcggagctcg
     3241 gggtgcaccg cttcgcgatg tgcttctcga acgtcgcctt catgggcttc ccggtggcgg
     3301 agtcgctcct aggcaaggaa agcctgttca tcctctcgat ctacaacata ccgttccagg
     3361 tactcgcctt ctcggtcggc atcgtgatga tatcggggcg acgctcgggc gccggtgcgc
     3421 cgggaacgcg ccgccccagc ctcggctcgc tccgttcact cgtcaacccg tcgatcgtgt
     3481 ccgcggtcgc gggcttcgcg ctgttcctcg gttcggtacg catacccgag ccgctgtact
     3541 cggcgatgga cctgctcggc ggcatgacca caccgctggc gatggtcgtc atcggcgcga
     3601 cgttggccaa cacgcgcatc ggcggagtcc tgtcgaaccc gcggctatgg ctgacgacgg
     3661 cctatcgcct cgccctcctg cccgccctgg cgttcgccgc ggccaaggcc gctggactta
     3721 cgggcctgga gctgtcgatt ccggtgctcg tggcggctat gcccgtggcg gccaatagca
     3781 ccatactcgc cggcgtctac ggcggcgacg acgtgaccgc ctcggccctg gtcttcgcct
     3841 cgacgctact gtcgctgttc accatccccg cgctcggcta cctgatagcc tgacccgagc
     3901 ctcggcgccg ctgttttaat cgatctgtct ctttaccctc ggattcgctc ggcgatatag
     3961 ttgcgccgta gcatcatgaa gacgaaccat ccggccggcc gccgcatagg cctgaaacga
     4021 ttcctatcgt acctatcgcc ctactggggc ctcttcctgc tcggcttcgc ggcgatggcc
     4081 ctatcgacgc tggcaaagct ggccggaccg ctcatcctgc gggccctcgt cgacgagtcc
     4141 ataccgaaca aggacctggt cggcatgata cgctacgccc ttctgtacct cggcatcatc
     4201 gtggcgatgg gggcgctcac ctactatcag acgacgacca tcgtcaagct ggggctgaac
     4261 gtcgtcacga acgtcaagcg agacttgttc t
//
LOCUS       DAOPHV010000181         4255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_28184_length_4255_cov_4.206905, whole genome shotgun sequence.
ACCESSION   DAOPHV010000181 DAOPHV010000000
VERSION     DAOPHV010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28184_length_4255_cov_4.206905"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLQ29_06030"
     CDS             <1..444
                     /locus_tag="PLQ29_06030"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HPG86242.1"
                     /translation="TDRDRELTGLPIRVAGSHAEISDKPRSKLISAGEPGRIAELAPV
                     LRARLAGLAEVVVSKPYFIEVLPAGADKGEALAWVAGVRGVPRESVMAIGDAGNDVGM
                     VSWAGVGCAPADARPEVLAVALHVSPLPHDSGAVADLIDRLVLRV"
     gene            504..692
                     /locus_tag="PLQ29_06035"
     CDS             504..692
                     /locus_tag="PLQ29_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86243.1"
                     /translation="MSQEMKDLGIKRCYACIQWTGQRTFYPDKKKIKVDEGSDGVCLM
                     THQKIKGSGHCEQYFPLR"
     gene            774..3083
                     /locus_tag="PLQ29_06040"
     CDS             774..3083
                     /locus_tag="PLQ29_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPG86244.1"
                     /translation="MTTVRIRILAASILAAAFALEASADIEFVGLDISPDDRILFSAR
                     VEVPGEPGYETLFSADAETGELVQLTFYPESIAVVDDGRRLQIRNRFGVFMTERGFGG
                     LAPLAGIPSFARGSAVQQGRLVDSRPSPDGTMLLYLSPTSPARGDLILFDLSVGRETT
                     VALSIEYSADSFPASWSPDSRYFVYGKGSEIYYFSTEQARAGRVPDESWRRVGAGRVS
                     QARWSANGSLYLLRDRSMYRIMPQEFFTQAIYSGIVQPGVLVGKAPFPYDPNFDSFWI
                     SPDGSKVLLCKDGRNIFLYRLDPDDFGREAAVNAMPYLFLQGNTVVDQVLWPSNDEVT
                     VFTASLREGKRVCGAYRVKAPGVGSESLSAGFQSLDVAGATEISLSPDESRVAIVTPD
                     GIVVRRYSNWAAERTLASPGALHAAWISQDRLAIASSGAIETVGVSDGSRTLVALGQP
                     GRFGWSEAEPGVVVAQTAGGAYKTGALKAAWSRAASFDPRPASTSSPGYRVYSDALAS
                     GSYRNTIMVRSAKGLGTRSLLPAPALAYKPFPERDDPRDPLIFDHGSRIRRREAALVF
                     NAYDGAEGLVGVLDTLREYGIEATFFVNGEFIRRNPGAAKLIAESGHEVGNMFFSVFD
                     ATDSRYRADAEFIKRGLARTEDEYFVATGAELSLLWHSPHYSTSTEILESAAQMNYTY
                     VGRDVDPLDWVGRFQGSLTQGLYSSAHDLVERAVAAVRPGSIVPIRIGIPDGGREDYL
                     YNELPLLINALLSEGYEIVPVSALMKHAE"
     gene            3122..4192
                     /locus_tag="PLQ29_06045"
     CDS             3122..4192
                     /locus_tag="PLQ29_06045"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG86245.1"
                     /translation="MLADSYRLDSRALDYSVSAADAARLDGGAWGDAVIGQPRALEAL
                     AIGTAIRAKGYNVFVTGAVGTGRRTAVMRTLAAYRPARLELRDVAYVYCFRSSTAPRA
                     LYFRAGEAAAFKKDLHAFVESVKKLVTLHAESGEAKRRREETEARWQKTENQRLAAFE
                     AELAADGFRVVQVQGDEGEVSTDILPLRNGEPVPFESVQAEAESGKIPEAEFSSLRER
                     YFAHMDEMRMLFVELRRGRAELEESVDGLRTQALQPLVHRDIELLASRYEDERTREWI
                     RALERDVMARLYLFQPQPPEAGHRSRLTRYGVNVLTDRDDLSAPPVVYEPNPTYANLF
                     GGLEPAGPDGQGPSRRCSSLRY"
BASE COUNT          668 a         1552 c         1385 g          650 t
ORIGIN      
        1 accgaccgcg accgcgagct gaccggcctg cccatacgcg tggccgggtc tcacgccgag
       61 atatcggaca agccgaggag caagctgatc tcggccggcg agccgggccg catcgccgag
      121 ctcgccccgg tcctgcgcgc gcggctcgcg ggcctcgccg aggtggtcgt ctccaagccg
      181 tacttcatcg aggtgctacc ggccggcgcc gacaagggcg aggccctggc ctgggtcgcg
      241 ggcgtccgag gcgtgccgcg cgagtcggtg atggccatcg gcgacgcggg caatgacgtg
      301 ggcatggtct cctgggccgg cgtcggctgc gccccggcgg acgcgaggcc cgaggtgctg
      361 gccgtcgccc tccacgtgtc gcccctgccc cacgactccg gcgccgtcgc cgacctgatc
      421 gaccgtctcg tcctccgggt ttgattgcct tcaactcccc ccgcccgcct gacgagaata
      481 agaacagacg gaaaggagcg agcatgtccc aggaaatgaa ggatctcggt atcaagcgtt
      541 gctacgcctg tatccaatgg accggccagc ggacgttcta tcccgacaag aagaagatca
      601 aggtcgacga ggggagcgac ggcgtctgcc tgatgaccca tcagaagatc aagggctccg
      661 ggcactgcga acagtacttc ccgctgcgct gatcccgcga taaggccacc cagggccggc
      721 ttcccctatc gctcgacggt tttttagccg aaaatggatc gaaggggaac gccatgacca
      781 ctgttcgtat ccgcatcctg gccgcctcga tcctagccgc cgctttcgcc ctcgaggcct
      841 cggccgatat agagttcgtg gggctggaca tctcgcccga cgaccggatc ctgttcagcg
      901 cccgcgtcga ggtgccgggc gagcccggct acgagacctt gttctcggcc gacgccgaga
      961 cgggcgagct cgtccagctg accttctacc ccgagtccat agcggtggtc gacgacggcc
     1021 gccgcctcca gatacgcaac cgcttcggcg tgttcatgac cgagcgcggc ttcggcggcc
     1081 tcgcgccgct ggccggcata ccgtcattcg cgcgcggctc ggcggtccag cagggccgcc
     1141 tcgtcgactc gaggccctcc cccgatggca ccatgctact ctacctgtcg ccgacgagcc
     1201 ccgcgcgcgg cgacctgatc ctgttcgacc tctccgtcgg cagggagacg acggtggccc
     1261 tgtcgatcga gtactcggcc gacagcttcc cggcctcgtg gtcgccggac agccgctact
     1321 tcgtctacgg caaaggctcg gagatctact acttctccac cgaacaggcg cgggccgggc
     1381 gcgtacccga cgagagctgg cgcagggtcg gagcgggccg cgtatcgcag gcgcgctgga
     1441 gcgccaacgg cagcctctac ctcctgcgcg accggtcgat gtaccgcatc atgccccagg
     1501 aattcttcac ccaggccatc tactcgggca tcgtccaacc gggcgtcctc gtcggcaagg
     1561 cccccttccc ctacgacccc aacttcgact ccttctggat ctcgcccgac ggctccaagg
     1621 tgctgctctg caaggacggc cgcaacatct tcctgtaccg actggaccct gacgacttcg
     1681 gccgagaggc cgcggtcaac gcgatgccgt acctgttcct gcagggcaac accgtcgtgg
     1741 accaggtgct ctggccgtcg aacgacgagg tgaccgtgtt caccgcctcg ctccgcgagg
     1801 gcaagcgcgt ctgcggcgcc tatcgcgtca aggcccccgg cgtcgggagc gagagcctgt
     1861 cggccggctt ccagtccctc gacgtggccg gcgccaccga gatcagccta tcgccggacg
     1921 agagccgggt cgcgatcgtc actcccgacg gcatcgtcgt caggcgctac tccaactggg
     1981 ccgccgagcg gacgctggcg tcgccggggg ccctgcacgc ggcctggata tcccaggaca
     2041 ggctcgcgat agcctcgtcg ggcgccatcg agacggtcgg cgtttccgat ggctcgagga
     2101 ccctcgtcgc gctcggccag cccggccgct tcggctggtc ggaagccgaa cccggcgtcg
     2161 tggtagccca gaccgccggc ggcgcctaca agaccggcgc cctgaaggcc gcctggtccc
     2221 gcgccgcgtc cttcgacccg cgcccggctt cgaccagttc gcccggctat cgcgtctact
     2281 ccgacgcgct ggcttccggc tcgtaccgca acaccatcat ggtgcgctcg gccaaggggc
     2341 tgggcacgag aagcctcctg cccgccccgg ccctcgccta caagcccttc cccgagcgcg
     2401 acgacccgcg cgatcccctg atcttcgacc acggctcgag gatcaggcgg cgcgaggcgg
     2461 cgctcgtgtt caacgcctac gacggcgccg agggactcgt cggcgtgctc gacacgctgc
     2521 gcgagtacgg catcgaagcc accttcttcg tcaacggcga attcatccgc cgcaacccgg
     2581 gcgccgccaa gctcatcgcc gagagcggcc acgaggtcgg caacatgttc ttcagcgtgt
     2641 tcgacgccac cgactcccgc taccgcgccg acgccgagtt catcaagcgc ggcctggcca
     2701 ggaccgagga cgagtacttc gtcgccacgg gcgccgagct gtccctgcta tggcactcgc
     2761 cgcactactc gaccagcacc gagatactgg aatccgcggc tcagatgaac tatacctacg
     2821 tcgggaggga cgtggatccg ctcgactggg tcggccgctt ccagggctcc ctgacccagg
     2881 ggctgtattc gagcgcccac gacctcgtcg agcgcgccgt ggcggcggtc aggcccggct
     2941 ccatcgtccc gatacggata ggcatacccg acggcggccg cgaggactac ctctacaacg
     3001 agctaccgtt gctgatcaac gccctgctct ccgagggcta cgagatcgtg ccggtctcgg
     3061 cgttgatgaa gcacgcggag taggccgcgc gccgcccgat ccctgaatga cggaggacac
     3121 gatgctcgcc gactcgtaca ggctcgactc acgggcgctc gactactcgg tatcggcggc
     3181 cgacgccgcc aggctggacg gcggagcctg gggcgacgcc gtcatcggcc agccgcgcgc
     3241 cctcgaggcg ctcgccatag gcaccgccat ccgagccaag ggctacaacg tcttcgtgac
     3301 cggcgcggtc ggtaccggcc ggcgtactgc ggtcatgcgg accctggccg cctaccggcc
     3361 cgccaggctc gagctacgcg acgtcgccta cgtctactgc ttccgctcat cgacggctcc
     3421 ccgcgcgttg tacttcaggg cgggcgaggc ggcggcgttc aagaaagacc tgcacgcctt
     3481 cgtcgagagc gtcaagaagc tcgtcaccct gcacgccgag tccggcgaag ccaagcgccg
     3541 ccgcgaggag accgaggccc ggtggcagaa gaccgagaac cagcggctcg ccgccttcga
     3601 ggcggagctg gcggccgacg ggttcagggt cgtgcaggtc cagggcgacg agggcgaggt
     3661 ctcgaccgac atcctgccgc tgcggaacgg ggagccggtg cccttcgaga gcgtccaggc
     3721 ggaggccgag tcgggcaaga tccccgaggc ggaattctcg tcgctccggg agcggtactt
     3781 cgcccacatg gacgagatgc gcatgctgtt cgtcgagctc aggcggggcc gcgccgagct
     3841 cgaggagtcc gtcgacgggc tacgcaccca ggcgctgcag cccctcgtcc accgggatat
     3901 agagctactc gcctcacgct acgaggacga gcggacccgc gagtggatac gcgccctgga
     3961 gcgcgacgtg atggcccggc tgtacctctt ccagccccag ccgccagagg ccggccatag
     4021 gagccgcctg acccgttacg gcgtcaacgt cctgaccgac cgcgacgacc tctcggctcc
     4081 gccggtggtc tatgagccca acccgaccta cgccaacctc ttcggcggcc tggagccggc
     4141 cggtccggac ggtcagggtc ccagccgccg gtgctcgagt ttacgatact gagatagcgg
     4201 tccgcgagcg cgctcgttcg ctcgccgtgg gcgtgacgcg ctacgcgctc tccgg
//
LOCUS       DAOPHV010000182         4252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_28209_length_4252_cov_3.839171, whole genome shotgun sequence.
ACCESSION   DAOPHV010000182 DAOPHV010000000
VERSION     DAOPHV010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28209_length_4252_cov_3.839171"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLQ29_06050"
     CDS             <1..594
                     /locus_tag="PLQ29_06050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPG86246.1"
                     /translation="EAFWNLYVPGCDEHYLCHILRDHPDFLPELDYVAEVDGRLVGSI
                     MYSRSYLIGDDGENVPIVSFGPVCVHPDYQRKGVGTALIGKTGAMAEAMGYPAIVIYG
                     DPHNYCKHGFRNGVDYRVSDMAGEHPLGLLVLELRPGFFGGTDWKARQSDAFMFDPAE
                     ALAFDEGFPPKEKKAQYSQELFSMQIRAFVKPTSVMS"
     gene            608..1591
                     /locus_tag="PLQ29_06055"
     CDS             608..1591
                     /locus_tag="PLQ29_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence protein RhuM/Fic/DOC family protein"
                     /protein_id="HPG86247.1"
                     /translation="MDSKIEIYKADNDQIQVSVRIENDTVWLNRHQLVTLFDRDIKTI
                     GKHITNIFAEGELEAESVVAYFATTAADGKTYQVEYYNLDVIISVGYRVKSKRGTLFR
                     QWATKRLKDYLVEGYAINQKRLEECNLELRHLKQGISILSRAIGQRARNLLEAKSLAT
                     LLERFSSGLEFLDDYDHETLDADGRTVQASERIDKAEYSSLIDAMRGEYSSDVFGKPK
                     DDSFDSSIRQIYQSFNGAELYRTIEEKAAMLLYLIVKNHSFVDGNKRIAAACFIYFLE
                     RNGLLYPEGATEPIIDSQALAAITLFIAVSKPEEIETVKKVLVTMLNRKFM"
     gene            1617..2189
                     /locus_tag="PLQ29_06060"
     CDS             1617..2189
                     /locus_tag="PLQ29_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86248.1"
                     /translation="MKGMYGMDGRVTYRRLVEGDRDAVAAIFVEAYYNQLRGLDPDRA
                     GLEAMFRGAFLLDHFTGAFDGDELVGFFALSTEAERSVAVRKDDVYARMRGVKRWFAY
                     AALRKEFGHPVRLREPGYYLECVAVKPSRQGEDRELRLPRARRPRYQSQGHRHLREAH
                     IHVPYEAPLRRGPGVSNGRQYRRHSRVEYL"
     gene            2146..2712
                     /locus_tag="PLQ29_06065"
     CDS             2146..2712
                     /locus_tag="PLQ29_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013136949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG86249.1"
                     /translation="MDVSIVDIAESNTYKLCAARTLVAAFADRERSAWPDIESAAEEV
                     EECAEGASLCIGAYDGRTMLGWVGLREMYKVTWELHPLAVDPRHQGKGIGSLLLRELE
                     ARAARRGIIGVMLGTDDEEFGTSLSAIDLDGGNVLREAERIRNLKRHPYEFYLKNGYA
                     IVGVVPNANGKRKPDIWMWKSLLNDREG"
     gene            2950..3672
                     /locus_tag="PLQ29_06070"
     CDS             2950..3672
                     /locus_tag="PLQ29_06070"
                     /inference="COORDINATES: protein motif:HMM:NF026198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HPG86250.1"
                     /translation="MPLIVGLRDNTLVGRTESYRFHSRCSGGVDFEDLVELMAEGRTT
                     VTAPDIRAVLELFRQTVAELAADGRYVRTPLGSFYLSAVGTAASPGASFTPRVKDSGH
                     ELRLRFRPDRGFEAMIAERAKVKRDDVSWRLFPRLSSLAQADGSAKEAASPGDILRLK
                     GAYLAFSQADPLQGVFMTRKDGSADTAAYRSERYLEARPSRVVFEVPPATPPGDYELS
                     LSSATKAGVLRSGILQETVRVG"
     gene            3715..4161
                     /locus_tag="PLQ29_06075"
     CDS             3715..4161
                     /locus_tag="PLQ29_06075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002687768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG86251.1"
                     /translation="MELAPMTIEDYDEVRDLWMNTPGMGLNTLDDSLEGIAKYLRRNP
                     TTCFVARIGKDVLGAILSGHDGRRGFIYHTAVRVSERNKGIGKRLVAKAIEALRNEGI
                     NKVAFVVFKENGTGNAFWERLGFDERTDLVYRNKTIATREMQRMDV"
BASE COUNT          943 a         1221 c         1312 g          776 t
ORIGIN      
        1 gaggccttct ggaacctcta cgtcccgggc tgcgacgagc actacctctg ccatatcctg
       61 cgcgaccacc cggacttcct gcccgagctc gactacgtgg ccgaggtcga cggcaggctc
      121 gtcggctcga tcatgtacag cagatcatac ctgataggcg acgacgggga gaacgtaccg
      181 atagtctcct tcggcccggt atgcgtccac cccgactacc agcgcaaggg cgtcggcacg
      241 gcgctgatcg gcaagaccgg ggccatggcc gaggccatgg ggtacccggc catcgtcatc
      301 tacggcgacc cccacaacta ctgcaagcac ggcttcagga acggcgtcga ctaccgcgtc
      361 agcgacatgg ccggcgagca ccccctcggc ctgctcgtcc tggagctcag gccaggcttc
      421 ttcggcggca cggactggaa ggccaggcag agcgacgcct tcatgttcga cccggccgag
      481 gcgctcgcct tcgacgaggg cttcccgccc aaggagaaga aggcccagta cagccaggaa
      541 ctctttagca tgcagatacg agccttcgtg aagccgactt cggtaatgag ctgaggggag
      601 atggagtatg gatagcaaaa tcgagattta taaagcggat aacgaccaga tccaggtgag
      661 tgtacgtatc gagaatgata ccgtttggct aaatcgtcac cagctggtca cgctcttcga
      721 tagggatata aagactatcg gaaagcatat tacgaatatc ttcgcggaag gcgaacttga
      781 ggcagaatca gttgtcgcat attttgcgac aactgccgcc gacggaaaaa catatcaggt
      841 cgagtattac aacctcgatg tcatcatatc cgtagggtat agggttaaat ccaagcgcgg
      901 gaccctgttt cgccagtggg cgacaaaacg gctcaaggac tatctcgtcg agggatacgc
      961 gattaatcaa aagcggcttg aagaatgtaa tcttgagctc cggcatctaa agcagggcat
     1021 atccatcctc agtcgcgcga tcggtcaacg cgcccgaaac ctgctagagg cgaagagcct
     1081 ggctactcta ctggagaggt tctcctcggg gctcgagttc ctagacgact acgaccatga
     1141 gaccttggat gccgatggta gaacggtaca agcgtccgag cgtatcgata aggctgagta
     1201 ttcgtccctg atcgacgcca tgcgcggcga gtactcttcg gatgttttcg gcaagcctaa
     1261 ggacgacagc ttcgatagct cgatacgcca gatataccag agctttaacg gcgcggagct
     1321 gtatcgaact atagaagaaa aagcggctat gctcctgtac ctgatagtta agaaccactc
     1381 cttcgtcgac gggaacaagc gcatagcggc cgcctgcttc atctatttcc ttgaacggaa
     1441 cggcctcttg taccccgaag gtgcaaccga acctataatc gatagccagg ccttggcggc
     1501 tatcacgctc tttatagctg tcagcaagcc tgaagagatt gaaactgtta aaaaggtgct
     1561 cgtgaccatg cttaatagga aattcatgta atgatctggt aggcagggcg agctggatga
     1621 aagggatgta cgggatggac ggtcgggtca cgtataggcg gctggtcgag ggcgataggg
     1681 acgccgtcgc cgctatattc gtcgaggcgt attataacca actacggggc ctcgacccgg
     1741 accgcgccgg cctcgaggcg atgttccgcg gcgccttcct gctcgaccat ttcaccggcg
     1801 ccttcgacgg cgacgagctc gtcggcttct tcgcgctgtc gaccgaggcc gagcgctcgg
     1861 tggccgtcag gaaggacgac gtgtacgcgc ggatgcgcgg cgtcaagaga tggttcgcct
     1921 acgcggcgct caggaaggaa ttcgggcatc ccgtacgcct gcgcgagccc gggtactacc
     1981 tggaatgcgt cgcggtcaag ccgagccgcc aaggcgagga tcgagaacta cgactacctc
     2041 gagctcgacg tcctcgatac caatcccagg gccatcgcca tctacgagag gcgcatattc
     2101 atgtaccata cgaagcgccc ctaaggcgcg ggccgggagt atcgaatgga cgtcagtatc
     2161 gtcgacatag ccgagtcgaa tacctataag ctgtgcgccg ccaggacgct cgtggccgcc
     2221 ttcgccgaca gggagcgcag cgcctggcca gacatcgagt cggcggcgga ggaggtagag
     2281 gaatgcgcgg agggcgctag cctatgcatc ggggcctatg acggccgtac catgctggga
     2341 tgggtcgggt tgagggaaat gtataaagtc acctgggaac tgcatccctt ggcggtcgat
     2401 ccccgccacc agggaaaagg tataggcagc ctgctgctac gggaacttga ggcgagggcc
     2461 gcccggcgcg ggatcatcgg cgtcatgctc ggcaccgacg acgaggagtt cggcacgagc
     2521 ctttcggcta tcgacctgga cggcggtaac gtactcaggg aagccgagcg catccggaac
     2581 cttaagaggc acccgtacga gttctacctg aagaacgggt acgccatcgt cggcgtcgtg
     2641 ccgaacgcta acggcaagag gaagccggat atctggatgt ggaagagcct gctgaacgac
     2701 cgggagggtt gaacctccgc cgggtccggc ggcgattctc gttgggcacg tcttgcttta
     2761 tttatactta gtcgtacaat ctagctagat cttaacaaaa tctagctaga tacggaaata
     2821 atctagctag atacgagatg aatctagcta gatacgaaac gaatctagct agatacgaaa
     2881 cgaatctagc tagattccgc aaagaacctt gttggatcga gggcgaccgt agcttggatc
     2941 ggaggccgta tgccgttgat cgtcgggctg agggataaca cgctcgtggg ccggaccgag
     3001 tcgtaccgct tccattcgcg ctgctccggc ggcgtcgact tcgaggacct cgtcgaactg
     3061 atggccgaag ggcggacgac ggtcaccgcg ccggatatcc gggcggtgct ggagctgttc
     3121 aggcagacgg tggccgagct cgccgccgac gggcgctacg tgcggacgcc cctgggctcc
     3181 ttctacctgt cggccgtcgg taccgccgcc tcgccgggcg caagcttcac gcctcgcgtc
     3241 aaagactctg gccacgagct caggctacgc ttcaggccgg accgaggctt cgaggccatg
     3301 atagccgagc gggccaaggt caagcgcgac gatgtatcgt ggcgtctttt ccccaggctc
     3361 agtagcctcg cgcaggccga cggctcggcc aaggaagccg cgagccccgg cgacatcctg
     3421 cgcctcaagg gcgcctacct ggccttcagc caggccgacc cgctccaggg cgtgttcatg
     3481 acgcgcaagg acggctcggc cgacacggcc gcctaccgct ctgaacgcta cctcgaggcg
     3541 cgccccagcc gcgtcgtgtt cgaggtccct cccgccaccc cgccggggga ctacgagctg
     3601 tcgctcagct ccgcgaccaa ggccggcgtc ctgcgttcag gaatattgca ggaaacggtg
     3661 agggtggggt gaagggggcc gccgcgagcg catagcctta gtaaggagcc cgagatggaa
     3721 ctagcgccga tgacgatcga agattacgac gaggtccgtg acctatggat gaataccccg
     3781 ggcatgggcc tgaatactct ggacgactcg cttgaaggca tcgcgaaata cctccgcagg
     3841 aacccgacta cctgcttcgt ggcgaggatc ggaaaagacg tgctcggagc catactgagc
     3901 ggccatgacg gcaggcgcgg cttcatctac cataccgccg tgagagtctc cgagcgaaat
     3961 aaaggaatcg gtaagcgact agtcgctaaa gcgatcgagg cgttacggaa cgaaggcata
     4021 aacaaggtcg cgttcgtcgt cttcaaggaa aacgggaccg gcaacgcgtt ctgggaaaga
     4081 ctcgggttcg acgagaggac cgacctggtc tataggaaca agaccatcgc gacgcgggag
     4141 atgcagagga tggacgtata gattccgagg cgacgactca ggcgtgataa ccgccgtttt
     4201 tccggccaca ttgtcatttg cattaactat gcttaccatt ccggccggca gc
//
LOCUS       DAOPHV010000183         4233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_28395_length_4233_cov_3.785543, whole genome shotgun sequence.
ACCESSION   DAOPHV010000183 DAOPHV010000000
VERSION     DAOPHV010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28395_length_4233_cov_3.785543"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PLQ29_06080"
     CDS             complement(<1..745)
                     /locus_tag="PLQ29_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HPG86252.1"
                     /translation="MRRSVALIVVALLVASASVVFADEAVLIDFSLLGADIIEDPVQA
                     GKLTQNRATMMDFSTVAGASYTEEQKKQMAISLAIENWDVILASSSRTGMRQALTYTR
                     EAKIAQDAKLYAGATALGIRVNFPLEPFNSWARIQPPFDVPAFEPKADIDDSGKITEK
                     TDAQGADPKNAALTRFEGSYDAETKITTALGIVKNVGVIKSIAVNVKGLNFPHGLSVI
                     LKDNDGVEHVMFMGYLNFDGWRELRWDNPQ"
     gene            complement(829..1368)
                     /locus_tag="PLQ29_06085"
     CDS             complement(829..1368)
                     /locus_tag="PLQ29_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86253.1"
                     /translation="MKVARQAMNVALKPRYFWLGALALALGASAIAGALSPPRQAAVL
                     WFPDAREADPTEARSELRYIGARKGVAEQAAELVEELLLGPMDAKARPIAVPQVRVES
                     AIVSNRTIYLDISADLFFGKPAGDGIYASPPVEPRVALEYIDRALRWNFRFYDVRLTV
                     QGLEPEWESVIEAETALNA"
     gene            complement(1389..2441)
                     /locus_tag="PLQ29_06090"
     CDS             complement(1389..2441)
                     /locus_tag="PLQ29_06090"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPG86254.1"
                     /translation="MRRSPLAALLLAFMAASAAWAQGGGEQWLDIKEVAARWKASVAY
                     DPLTGTGHVLRGTDLVRFKLDYPVFSVGQGTALRATAIKDEGIRLGIRSDAVRAIESW
                     FAERDAERASHFSVAAILIDPGHGGRDPGAIGEFGSGKDRLRVAEKDVALAIGRDVYD
                     RLVERWPDKRIIITRRGDTFPTLDERVEMANDVQLGINEAIIYVSIHANASFNKSASG
                     FEVWYLNPEYRRTVVDPMKAQGVDEHVLPILNAMLEEEYTTESVFLAKTILGGLAGVV
                     GEVSANRGLRAEEWFVVRNAKMPSVLVEVGFVTNEEEARRLSDPGHLRKLGDGIYNGI
                     VEFVDYFEHRKGSSAQ"
     gene            complement(2444..4018)
                     /locus_tag="PLQ29_06095"
     CDS             complement(2444..4018)
                     /locus_tag="PLQ29_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPG86255.1"
                     /translation="MRSILILGAGFMQGPAIRIAKAKGYRVYAADADPNATHVGSVDE
                     FLRVDLKDKEGLATAAEGIPDLAGVFTAGTDFSASVAYVAERLGLPGIPYEAALDASD
                     KARMRSRLRAAGVPVPDYAFGGSEDDPVALAEAVGSWPVVVKPVDNMGGRGCRLARSV
                     SELRDAWADAVSHSRTGRAIVEAYLDGPEFSIDAIVRDGIVSLRGVADRVVVFEPYFV
                     EMGHTMPTAYGEEVTGEVVAVFEAGVAALGIRNGAAKGDMKYTRRGAFVGEIAARLSG
                     GYMSGWTYPYSSGLEPTAEAIDIACGVVPAEPGTPLGLSCSERAWISIPGRVAEIRGL
                     AAAEAMPGVRHVFPRASAGDRVVFPSNNVQKCGNVIAVGGSVAEADAYAEAAARSILI
                     RLEPDDAATADFLRGNGSLLGPDGSVWPPLAFGGVSAMTESSLHKLPGMVRLNETGGS
                     VLIAPLRGIEAESARDWQGRSISEALEAVAELTGARIGLDGDLVLGSCFWRAFLRGGY
                     QGGAWAVERARLGDRG"
     gene            complement(4015..>4233)
                     /locus_tag="PLQ29_06100"
     CDS             complement(4015..>4233)
                     /locus_tag="PLQ29_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86256.1"
                     /translation="ILKRFGFDVQRIVVTGHHPERFPGVPAEADSLRYGAAMTVSRLL
                     GLGDTFECYAVYRGRVEERVARRSAERT"
BASE COUNT          717 a         1393 c         1372 g          751 t
ORIGIN      
        1 actggggatt gtcccagcgc agctcgcgcc agccgtcgaa gttcaggtag cccatgaaca
       61 tgacgtgctc gacgccgtcg ttatccttga ggatgaccga caggccgtgc gggaaattca
      121 ggcccttcac gttgacggcg atcgacttga tcacgccgac gttcttgacg atgccgaggg
      181 cggtggtgat cttggtctcg gcgtcgtagc tgccctcgaa gcgggtcagc gcggcgttct
      241 tgggatcggc gccctgggcg tcggtcttct cggtgatctt gcccgaatcg tcgatatcgg
      301 ccttaggctc gaaagccggg acgtcgaacg ggggctggat cctggcccag gagttgaagg
      361 gctcgagcgg gaagttgacg cggatgccga gggcggtggc gccggcgtac agcttggcgt
      421 cctgggcgat cttggcctcg cgggtatacg tgagcgcctg gcgcatgccg gtcctggagg
      481 acgaggcgag tatcacgtcc cagttctcta tggccaggga gatggccatc tgcttcttct
      541 gctcctcggt gtagctcgcg cccgcgaccg tcgagaaatc catcatggtg gcgcggttct
      601 gcgtcagctt accggcctgg acggggtcct cgattatatc cgctccgagg agggaaaaat
      661 ctatcaggac ggcctcatcc gcgaaaacga ccgatgcgct cgcgaccaac agagcgacga
      721 cgatcagtgc tactgacctt ctcattcaaa tgctcctttc catagtgcgc gtacagtttt
      781 acccaatttg acttgattat agccaagccc taccctttgt caattatttt atgcatttag
      841 ggccgtttcg gcctcgatta ctgactccca ctcgggttca aggccctgta ccgtcaggcg
      901 gacatcgtag aatctgaagt tccagcgtag ggccctgtcg atatattcca gggcgacgcg
      961 cggctcgaca ggcggcgagg cgtagattcc atcgccggct ggcttgccga agaagaggtc
     1021 ggccgatata tcgaggtaga tagtcctatt cgagacgatg gcagactcga ccctgacctg
     1081 cggaacggcg ataggcctgg ccttcgcgtc catcggcccg agcagcaact cctcgacgag
     1141 ctcggcggcc tgttcggcta cgcccttacg agcgccgata tatcgtagct ccgaacgcgc
     1201 ttcggtcgga tcggcttcgc gcgcgtcggg aaaccatagc accgcggcct gcctgggcgg
     1261 cgacagcgct ccggctatgg cgcttgcgcc gagagccaaa gccagcgccc cgagccagaa
     1321 atatctcggt ttaagcgcca cattcatcgc ttggcgggca accttcacga tccgttcgag
     1381 agtcggtttc actgggcgct cgagcctttc ctgtgttcga aatagtcgac gaactcgacg
     1441 atgccattat agatgccgtc gcccagctta cgcaagtgac cggggtcgct caggcgcctg
     1501 gcctcctcct cattggtgac gaagcccacc tctacgagca cggacggcat cttggcgttc
     1561 ctgacgacga accattcctc ggcgcgtagt ccgcggttgg ccgagacctc gccgacgacg
     1621 ccggccagcc cgccgagtat ggttttggcc aggaatacgc tctcggtcgt gtactcctcc
     1681 tcgagcatcg cgttgagtat cggtagcacg tgctcgtcga cgccctgtgc cttcatcggg
     1741 tcgacgaccg tgcgccggta ctccgggttg aggtaccaga cctcgaagcc cgaggccgat
     1801 ttgttgaacg acgcgttggc gtggatcgat acgtagatga tggcctcgtt gatgccgagc
     1861 tgtacgtcgt tggccatctc cacgcgttca tccagagtcg ggaaggtatc tccgcggcgc
     1921 gttatgatga tgcgcttgtc cggccaacgc tcgaccagcc ggtcgtagac gtcgcggccg
     1981 atggcgagag cgacgtcctt ctcggcgacg cgcagcctgt ccttgcccga gccgaactcg
     2041 ccgatggcgc cgggatcccg gccgccatgt ccggggtcga tcaggatggc cgccacgctg
     2101 aaatgcgagg cccgctcggc gtcccgctcg gcgaaccaag actcgatagc ccggaccgcg
     2161 tcggaccgta tgcctagccg tatgccctcg tccttgatgg ccgttgctct gagcgccgtt
     2221 ccctggccga cggagaagac cggatagtcg agcttgaagc gtacgaggtc cgtgccgcgc
     2281 aggacgtgcc cggtgccggt cagggggtcg taggctacgc tggccttcca gcgcgcggcg
     2341 acctccttga tgtcgagcca ctgctcgccg ccaccttgcg cccaggccgc cgaggccgcc
     2401 atgaacgcca atagcagcgc ggcgagtggc gagcggcgca tcctcatcct cggtccccca
     2461 ggcgggcgcg ctcgacggcc caggcgccgc cctggtagcc gccgcgtagg aaagcccgcc
     2521 agaaacacga gccgagcacc aagtcaccgt cgagccctat cctggcgccg gtcagctccg
     2581 ccacggcctc gagcgcctcg gatatcgagc gaccctgcca gtcccgggcg ctctcggcct
     2641 ctatgccgcg caggggcgct atcaagaccg atccgcccgt ctcgttcagc ctgaccatgc
     2701 cgggcagctt atggagcgac gactcggtca tcgccgatac gccgccgaag gccaggggtg
     2761 gccagacgga gccgtcgggg ccgaggagcg agccattccc tcgcaggaag tcggcggtgg
     2821 ccgcgtcatc gggctcgagc cgtatcagga tggacctggc ggccgcctcg gcgtaagcgt
     2881 cggcctcggc gactgagccg ccgaccgcta tgacgttgcc gcatttctga acattattgg
     2941 acgggaaaac cacgcggtcg cctgcggagg ccctcgggaa gacatgcctg acgccgggca
     3001 tcgcctcggc tgcggccagg cccctgatct cggcaacgcg gccaggtatc gatatccagg
     3061 cgcgctccga gcagctcagg ccgagtggcg tcccgggctc ggccggcacg acgccgcagg
     3121 cgatgtcgat ggcctcggcc gtcggctcga gtccgctcga gtacggatag gtccagcctg
     3181 acatatagcc gccggacagc ctcgcggcaa tctcgccgac gaaagcgccg cgcctcgtgt
     3241 acttcatgtc gcccttggcc gcgccgttcc ggataccgag agccgcgacg cccgcctcga
     3301 agacggcgac cacctcgccc gtcacttcct cgccgtaggc cgtgggcatc gtatgcccca
     3361 tctcgacgaa gtacggctcg aagacgacga cgcgatcggc gacgccgcgc agagagacga
     3421 tgccgtcacg cacgatggca tcgatactga actcggggcc gtcgaggtag gcctcgacga
     3481 tggcgcgccc ggtgcgagaa tgcgagacgg cgtcggccca ggcgtcgcgc aactccgaga
     3541 cggatcgagc cagacggcag ccacggccgc ccatgttatc gaccggcttg acgacgaccg
     3601 gccaggagcc gaccgcctcg gccaaggcca ccgggtcgtc ctccgagccg ccgaaggcgt
     3661 aatcgggcac cggcacgccg gccgcccgga gcctcgaacg catcctggcc ttatcggatg
     3721 cgtcgagggc cgcctcgtag gggatgccgg gcaggccgag ccgctcggcg acgtaggcta
     3781 ccgaggcgga gaagtcggtg ccggcggtga agactccagc gaggtcggga attccctcgg
     3841 ccgcggtcgc gaggccttcc ttgtccttga ggtcgacgcg caggaactcg tcgaccgagc
     3901 cgacatgggt cgcgttcggg tcggcgtcgg ccgcgtagac tctgtagccc ttggccttgg
     3961 ctatccggat ggccggcccc tgcatgaagc ctgctcccaa gatcaatatg gatctcatgt
     4021 tcgctccgcg gatcgccggg cgacccgttc ctctaccctg ccgcgataga cggcgtagca
     4081 ctcgaaggtg tcgccgagcc ccaacagtct cgatacagtc atagcggccc cgtagcgcag
     4141 cgaatccgcc tcggcgggta cgccggggaa gcgctcagga tggtggccgg tgacgacgat
     4201 acgttgtaca tcgaaaccga agcgcttgag aat
//
LOCUS       DAOPHV010000184         4214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_28560_length_4214_cov_3.736716, whole genome shotgun sequence.
ACCESSION   DAOPHV010000184 DAOPHV010000000
VERSION     DAOPHV010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28560_length_4214_cov_3.736716"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..952)
                     /locus_tag="PLQ29_06105"
                     /pseudo
     CDS             complement(1..952)
                     /locus_tag="PLQ29_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694577.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
     gene            1309..2517
                     /locus_tag="PLQ29_06110"
     CDS             1309..2517
                     /locus_tag="PLQ29_06110"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPG86257.1"
                     /translation="MRARLSKAVAPILIVAAFASLFAFSLAALAGMDREYREMQLGME
                     RSLRAVLRAVPAGRSLPPELEGLFAAVERLERIAVSVDLEEPRRALLRLVELYEPGLT
                     DTEAFLDRFCESYAILETDLARKRAALADSFYALIAALAIGFVVAAAIGATLSLRLQV
                     SRLEAVWSRRSLLASLRAEEGLRKRIARDLHDDVAQDLAAARMLCERAASPSDASDGQ
                     LTRLLSESIGLLTSAGKRLRGLALDLRPPELERSGLAAAIESLCSRSFGQHLRLATFR
                     AEAGAAEAADSLDDEAGIQVFRIVQEALANARKHAPGQAVDVSLRLDGEPSAEALLVE
                     VVNRLDAAADAAELVRLDESVSSGLGQAIMRERAAFAGGELRAGTEAGSYRVTLRVPF
                     TKTGKAGLDA"
     gene            2510..3163
                     /locus_tag="PLQ29_06115"
     CDS             2510..3163
                     /locus_tag="PLQ29_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPG86258.1"
                     /translation="MRKALIVDDHPVFRNGLKTAFEGWRIFEAIDEAGSAAEAREALD
                     RGGYGLAMVDVGLPDGSGMDLIESRAGLPGTPAFFVLTMEADASLARRAIRAGATGFA
                     SKNLALGVLELAVRLVMAGEPYIEGEILRDLLTAEAILSGARHEGRRLVGGLTPRERS
                     TLDALLEGLNAKEIAQRLGVSHRTAENYQSAVYAKLGARSPVALVRIALDAGLIASL"
     gene            complement(3173..3751)
                     /locus_tag="PLQ29_06120"
     CDS             complement(3173..3751)
                     /locus_tag="PLQ29_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86259.1"
                     /translation="MTLVVSRKPILAWGLAIVVFPLANALLSLAARDALPLFFDSILT
                     AVAAAVLGPWHGLATAILTNGWSEALDGFPGVHLPFAVCGIATALIVRAFVASGKYRG
                     ALPVSLCVGAVTLANALLGALIAAFVYGGGTRMNLDNIVAGFALFTDSVLTAAFLGRL
                     PINLVDKTLAVAPALYLAIVKAAAAAAGQDER"
BASE COUNT          684 a         1349 c         1474 g          707 t
ORIGIN      
        1 ccttgaggaa atgctcgacg gagaaggggc ggtacaggcg gacgatgacg gcgccgacct
       61 tctcgccctt cttgacgagg tggttgacgg tctcggtcac ggtgtcggcg ccggagccca
      121 tcacgatcaa cacgcgctcg gcgtcggggg cgccgatata gtcgaacagg tggtactggc
      181 ggccggtgtg cttggccagc ttgtccatgt acttctgcac gatggccggg gtggcctcgt
      241 agtacttgtt gaccgcctcg cggccggtga agtacacgtc ggggttctgg ctggtgccgc
      301 ggatgaccgg cttctcggga ttgaggccgc gggcgcggtg ggcgcggacg agctccgcgt
      361 cgatcatgtc cttcatgacc tcgtaggata tctcctcgac cttctggacc tcgtgcgagg
      421 tgcggaagcc gtcgaagaag tgcaggaacg gcacgcgggc ctcgagggtc gcggcgtgcg
      481 cgatcagggc catgtccatc acttcctgga cgctggcggc gccgaggagg gcccagccgg
      541 tctggcgggc ggccatgatg tcctggtgat cgccgaagat cgacagggcg ctggcggcta
      601 cggcgcgggc ggcgatatgg aagacggcgg aggtacactc gccggctatc ttgtacatgt
      661 tcgggatcat caggaggagt ccctggctgg cggtgaacgt cgtggtcagg gcgcccgtgg
      721 tcagcgctcc gtggacggcg cccgcggctc cggcctcgga ctgcatctcg acgacctgcg
      781 gcaccgtgcc gaagatgttc ttacggccct gggcggagaa ttcgtccgac agctcaccca
      841 tcggcgagct gggggtgatc gccatctacc cgatcacctc ggagcaggcg tgggcgacgt
      901 gcgcggcggc ggtgttgccg tcgatcatca ccatgttctt cttagtagcc atacgggttc
      961 ctttggggtg tagttgcccg ggcgtggcgc ggtcttccgg cgggactgaa cgtgagtacg
     1021 gcgcgcgttg ctatgggcag ctatatagta ggttttttgt gtaacttgcg caagggatgc
     1081 atacgcgatc cccgggctta atggatataa aaggcgcaaa agaggggcgc tccgccccct
     1141 cgacgataga gcgctacgaa agcgcgcctg gcggcgcgcc ggcgagccgt cgcgcgacga
     1201 ctcgcctcct catcctggcg taacgattcg attgcccgct tcccggccgt ggtctatact
     1261 cgactctcgc gcctcggatc catgagcccg tggcggcaag gagcgttcgt gcgggccagg
     1321 ctttctaagg cggtagctcc catcctcatc gtggcggcct tcgcgtcgct gttcgccttc
     1381 tcgctcgcgg ctctggccgg catggaccgg gaataccggg agatgcagct gggcatggag
     1441 cgctcgctcc gggcggtgct gcgcgccgta cccgccggcc gctccctgcc gccggaactc
     1501 gaagggctgt tcgccgcggt cgagcggctg gagcgcatag ccgtcagcgt cgacctggaa
     1561 gagccgcggc gcgccctgct ccggctcgtc gagctgtacg agccaggtct gaccgatacc
     1621 gaggcgttcc tggaccgctt ctgcgagtcg tacgccatcc tggagacgga cctggccagg
     1681 aaacgggccg cgctagccga ttcgttctac gcgctcatcg ccgccctggc catcggcttc
     1741 gtcgtggcgg ccgctatcgg cgccaccctg agcctgcgcc tgcaggtcag ccggctcgag
     1801 gccgtctggt cgcgcaggag cctgctcgcg tcgctacggg ccgaggaagg gctacgaaag
     1861 cgtatcgccc gcgacctcca cgatgacgtg gcccaggacc tggccgccgc caggatgcta
     1921 tgcgagcggg ccgcttcccc gtccgacgcc tccgacggcc agctgacccg attactctcc
     1981 gagtccatcg gcctcctgac ttcggcgggt aagcgcctgc gcggcctggc cctggacctg
     2041 aggccgccgg agctggagcg ttccgggctc gcggcagcca tcgagtcgct gtgctcgagg
     2101 agcttcggcc agcatctcag gctggctacg ttccgcgcgg aggccggcgc cgccgaggcc
     2161 gccgactcgc tcgacgacga ggcgggcata caggttttcc gtatagtcca ggaggcgctc
     2221 gccaacgcgc gcaagcacgc gcccgggcag gccgtcgacg tgtcgctgcg gctggacggc
     2281 gaaccgtccg ccgaggcgct cctcgtcgag gtggtcaacc gcctcgacgc cgcggccgac
     2341 gccgccgagc ttgtgcggct cgacgagtcc gtatcgagcg gcctcgggca agccatcatg
     2401 cgggagcgcg ccgccttcgc cggaggcgag ctgcgcgctg gaaccgaagc cggatcatac
     2461 cgggttacgc tacgcgtacc gttcacaaaa actggaaagg cgggtctcga tgcgtaaagc
     2521 cctgatagtc gacgatcatc ccgtattcag gaacgggctc aagacggcct tcgaaggctg
     2581 gcgtatcttc gaagcgatcg acgaggccgg ctcggcggcc gaggcccgcg aggccctgga
     2641 ccgcggcggc tatggcttgg ccatggtcga cgtcggcctg ccggacggca gcggcatgga
     2701 cctgatcgag agccgggcgg gcctgcccgg aacgcccgcc ttcttcgtgc tgaccatgga
     2761 ggccgacgcc tcgctcgccc ggcgcgccat acgggcgggc gccacaggct tcgcgtccaa
     2821 gaacctcgcg ctcggcgtac tcgagcttgc ggtcaggctc gtgatggccg gcgagccgta
     2881 tatcgagggc gagatactgc gcgacctcct gaccgcggag gccatcctgt ccggcgccag
     2941 gcatgaggga cggcggctgg tgggcgggct gaccccgcgc gagcggtcca ccctcgacgc
     3001 gctgctcgaa gggctgaacg ccaaggagat cgcgcagagg ctcggggtca gccaccgcac
     3061 cgcggagaac taccagagcg ccgtgtacgc caagctcggc gccaggagcc ccgtcgccct
     3121 cgtgcgcatc gcgctcgacg ccgggctgat cgcctctctg tagcgaccgg gcctagcgct
     3181 cgtcctgacc ggccgccgcg gcggccgcct tcacgatggc caggtacaaa gccggcgcca
     3241 cggccagcgt cttgtccacc aggttgatcg gcagcctgcc caggaaggcc gcggtcagca
     3301 ccgagtcggt gaatagcgcg aaaccggcga cgatgttgtc gaggttcatc cgcgtgccgc
     3361 cgccgtagac gaaggccgct atcaaggcgc cgagcagcgc gttggccagc gtaaccgcgc
     3421 cgacgcacag cgataccggc agggcgccgc ggtacttgcc cgaggcgacg aaggcccgga
     3481 cgatgagggc ggtagctatg ccgcagacgg cgaacggcag atggacgccg gggaagccgt
     3541 cgagcgcctc gctccagccg ttcgtcagga tcgccgtcgc taggccgtgc catggcccca
     3601 ggaccgccgc cgcgacggcg gtgaggatcg agtcgaaaaa caggggcaac gcgtcccggg
     3661 cggccaggct caggagggcg ttggccagcg ggaagacgac gatggccaag ccccaggcca
     3721 ggatgggctt cctggaaacg acgagcgtca tggcgcccaa ggatcgtggt tcggtttcgc
     3781 gcattcctca ttatagaacg gtactacgga atatgcttcg ttattttatc gatagccaga
     3841 gttaatccgt gagaatccgc aacggggcga cgcttttcgt cgcttgaagc atgggcgtcg
     3901 tggttgtagg atgcccgcat cggcatcctg agccgataaa acaatggcaa aaacgcttat
     3961 tgaatccggg gacctcgcga ggggtccccg tttttatcgc gtcgcgtcgc gctatcgccc
     4021 gtcggtgagt aaccgctcac atattttgaa accgtaaagg tatggatgga gggcttcggt
     4081 taattcaacg aagaggatat aagaatacga ggtaggagcg aaaggtcgat acagggatgg
     4141 gggagcgggc caaggaagtt ccatcgctcc ccctagtcct ttttatcacg ccccgagggc
     4201 ggacagggca gcgc
//
LOCUS       DAOPHV010000185         4207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_28625_length_4207_cov_3.793112, whole genome shotgun sequence.
ACCESSION   DAOPHV010000185 DAOPHV010000000
VERSION     DAOPHV010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28625_length_4207_cov_3.793112"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="PLQ29_06125"
     CDS             <1..1112
                     /locus_tag="PLQ29_06125"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HPG86260.1"
                     /translation="EYEASPDVVVSAPGCILLLGEHTADSDGLMIAFPMNRRVSVAVS
                     GRRDSSIRFYAADLNERKRTNLSNLKYKREDRWANFIKAALSTYAGEPSGLKGFNITV
                     SGDIPQALGLGSATALQCAAAKAAAIAAGFDPSPEELARAIVRTDRGYFERDTTMAVY
                     LATLAGSEGAMVYVDSAAESGEALPSVFTGARLILTDSKVPRPLVDSEMRQRGEDCAR
                     GLAMMDGSGSRRLRDFSIDDLDEYMGLMPERVRRHCAFFVEEVQRVKEAREAALHTDL
                     QTFAKILNKSQSGLRNSYEISCPEIDWLVKRAVEIDGVLASRMIGKGFGGCTISAMSD
                     RAMEEYRSRLEEYERIFGFKPHALEISVGAGMIAV"
     gene            1144..1866
                     /gene="surE"
                     /locus_tag="PLQ29_06130"
     CDS             1144..1866
                     /gene="surE"
                     /locus_tag="PLQ29_06130"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPG86261.1"
                     /translation="MRILLTNDDGYASDGIKALAKALEGEHEVWTLAPDSERSGMSHS
                     MSLRQPLKIRKLAEREYCCSGTPADCVILASHGVLPFSPDVVVSGINRGPNLGTDLVY
                     SGTAAAARQAAMNGVPGIAVSLATYTGPFIYDAAAQMIAERLDYFISLWTPDVFVNIN
                     APVAPAGHRYEVIEAFPSRRVYKDTLKFFDGPDGYSYCFFMDGQVDTHQESGSDEDAI
                     GKGLAAVTRVLAYPCAAGGAAS"
     gene            1863..2558
                     /locus_tag="PLQ29_06135"
     CDS             1863..2558
                     /locus_tag="PLQ29_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG86262.1"
                     /translation="MKKGKPSGSGAIAKAGNEYRSGDYDAAVLTLGQATVDEDEYVDL
                     AYLLGLSYARLGRFDEALLYLEQVVTQSVPDRRVDQCRMTLAYVYAVTGRAKLAEYEL
                     KKLLESDKRSPQVFSALGHASWKQGKLDDGIAWYAKALELEPENPTALNGYGYLLACA
                     DRDLQKALTCCRKALDSDPGNPAYADSIGWTYLRLGMLAEAGRFLGQAAAELDDEELR
                     EHLETYEKAVGSR"
     gene            2555..>4207
                     /locus_tag="PLQ29_06140"
     CDS             2555..>4207
                     /locus_tag="PLQ29_06140"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG86263.1"
                     /translation="MRRLVATLTIAVAVSAAGAQFAYEIDAPLAAESFRTGVEAFGRG
                     YYAESLAQLERALASEPRDPLILYWLGKAYLRLGLPSAAFERWGDALSVAGGSPFVES
                     RLELAGAMASPEGLAEPTRYVRVAELSGRKGDTVLFSRPTWIEPLPDGKAIVVSHGTD
                     RLLVVDANGRIVDTLNAGSAGFDRPFACARLDDGTLFVTEFQADRIARLSPDGRVLGY
                     SGDASGPGRLSGPQYITADADGFLYVSDVGFSRVVKYARDGSMLLSFGARNPTFDGLS
                     LPTGVAVVGDVVYVADAALKAIFLFDRYGNYLGRARVPGLVRPEGLRATGDGRLLVAD
                     GSRVLLVDPESAATIELYRSERKRPRILSAAFDANGELMAADFDASEIAYLSDPASRF
                     AGLSVEVGRVYSDSFPRVSLDVTVKDRHGRPVTGLGYPNFYISELVKTKERRVEGDKP
                     VDYIASSIRPAGELSFDGSLDQSSGIDLTFLLEGSPQLSPMRLEARDAAYAVYESLGG
                     GTDSMARLVVAGRVAQPPSAANVAAIGAAALGYEPSSDWRFDS"
BASE COUNT          722 a         1420 c         1342 g          723 t
ORIGIN      
        1 cggagtacga agcgtctccc gacgtcgtcg tttcagctcc cggctgtata cttctcttag
       61 gcgagcatac cgccgacagc gatggcctca tgatcgcctt cccgatgaac aggcgtgttt
      121 cggtagccgt atccggacga cgggactcgt cgatacgctt ctacgcagcc gacttgaacg
      181 aacgtaagcg tacgaaccta tcgaatctca agtataagcg agaagatcgc tgggccaact
      241 tcatcaaggc ggccctgtcc acctacgccg gcgaaccgtc ggggctcaaa ggcttcaata
      301 ttacggtatc cggcgacatt ccccaggctc tcggcttggg ctcagcgact gcactgcagt
      361 gcgcggccgc gaaggcggcc gccatagcgg ccggcttcga cccgtcaccc gaggagctgg
      421 cccgcgccat cgtgcgaacc gatcgcggct atttcgagcg tgacacaacg atggccgtct
      481 acctcgccac gctcgccggc tcagaagggg ccatggtcta cgtcgactcg gcggccgaaa
      541 gcggcgaggc cctgccttcg gtcttcacgg gcgcgcgcct tatcctgacc gactcgaagg
      601 taccgaggcc gctcgtcgat agcgagatgc gccaacgcgg cgaggactgc gccagaggcc
      661 tcgcgatgat ggacggctcg ggttccagac ggctcaggga tttctcgatc gacgacctcg
      721 acgagtacat gggcttgatg ccggagcggg tccgacgcca ttgcgccttc ttcgtcgagg
      781 aggtccagcg cgtcaaggag gctcgggagg ccgccctgca caccgacctt cagaccttcg
      841 cgaagatctt gaataagtcc cagtcgggcc tcaggaacag ctacgagata tcgtgtcccg
      901 agatagactg gctcgtgaag cgcgccgtcg agatagacgg cgtgctggcc agcaggatga
      961 taggcaaggg attcggcggg tgcaccatca gcgccatgtc cgaccgggcc atggaggagt
     1021 accggtcgcg cctcgaagaa tacgagcgta tcttcggctt taagccgcac gccttggaaa
     1081 tatcggtcgg ggctggtatg atcgccgtat agcgatcgcc cgagcaaggg cccaggagct
     1141 tcgatgagaa ttctactgac gaacgacgac ggttacgcga gcgacggcat caaggccctc
     1201 gccaaggcgc tcgaaggcga gcacgaggtc tggacgctgg cgcccgactc ggaacgctcc
     1261 ggaatgagcc attcgatgtc cctcaggcag ccgctcaaga tacgcaagct agccgagcgc
     1321 gagtactgct gttccgggac gccggccgac tgcgtcatac tcgcgtcgca cggcgtgctt
     1381 ccgttctccc cggacgtcgt cgtatccggt atcaaccggg gcccgaacct cggcaccgac
     1441 ctcgtatact cgggtaccgc cgcggccgct cggcaggctg ccatgaacgg cgtaccgggc
     1501 atcgccgttt cgctggcaac ctataccggt ccgttcatct acgatgcggc cgcccagatg
     1561 atagccgagc ggctcgatta cttcatatcg ctatggacgc ccgacgtttt cgtcaacata
     1621 aacgcgccgg tcgccccggc ggggcaccgc tacgaggtca tcgaggcctt cccgagccgc
     1681 cgcgtctaca aggatacgct caaattcttc gatggccccg acgggtactc ctactgtttc
     1741 ttcatggatg gccaggtgga cacgcaccag gagtcgggca gcgacgagga cgcgatcggc
     1801 aagggccttg cggcggtcac acgggtgctg gcgtatccat gcgcggcggg cggcgcggcg
     1861 tcgtgaaaaa gggcaagccg tcgggttcgg gcgccatcgc gaaggccggt aacgaatacc
     1921 ggtccgggga ttacgatgcc gccgtcctga cgctcggcca ggcgaccgtc gacgaggacg
     1981 agtacgtcga cctggcctac ctgcttggcc tctcgtacgc caggctcggg cgcttcgacg
     2041 aggcgctcct gtacctggag caggtcgtca cgcagtcggt gccggaccgt cgggtcgacc
     2101 aatgccgcat gacgctcgct tacgtgtacg ccgtcaccgg tcgcgccaag ctggccgagt
     2161 acgagctcaa gaagctgctc gagtccgaca agcggtcgcc ccaggtcttc tcggcgctcg
     2221 gacacgccag ttggaagcag ggaaagctcg acgacggcat cgcgtggtac gccaaggccc
     2281 tcgagctgga gcctgaaaat cccacggcgc tcaatggcta cggctacctg ctcgcctgcg
     2341 ccgaccgcga tctgcagaaa gccttgacct gttgccgtaa ggccctcgat tccgatcccg
     2401 gcaacccggc ctacgccgac tcgataggat ggacctatct gaggctcggc atgctcgccg
     2461 aggccggcag gttcctcggg caggccgcgg ccgagctcga cgacgaggaa ctacgagagc
     2521 acctcgaaac ctacgaaaag gcggtcggat cccgatgaga aggctcgtcg caacgttgac
     2581 catcgccgtc gccgtgtcgg ccgcgggcgc ccagttcgcc tacgaaatag acgccccgct
     2641 cgccgccgag tcgtttcgca ccggcgtcga ggcgttcggc aggggctatt acgccgaatc
     2701 gctcgcccaa ctcgagcggg ccctggcgag cgagcctcgc gatccgctga tactctactg
     2761 gctgggaaaa gcctatctca ggctcggcct ccccagcgcg gcgttcgagc gctggggcga
     2821 cgcgctgtcc gtcgccggcg gctcgccctt cgtcgagagc aggctcgagc tggccggggc
     2881 catggcctcg cccgagggcc tggccgaacc gacccgctac gtccgcgtcg ccgagctgtc
     2941 aggcaggaaa ggcgacacgg tgctgttctc gaggccgacc tggatcgagc ccttgcccga
     3001 tggcaaggcc atcgtcgtct cccacggcac cgaccgcttg ctcgtggtcg acgccaatgg
     3061 ccgcatcgtc gataccctga acgccggctc ggccggtttc gacaggccct tcgcctgcgc
     3121 caggctcgac gacggcacgc tcttcgtcac cgagtttcag gccgacagga tagcccgcct
     3181 gtcgcctgac ggccgcgtgc tcggctattc gggcgacgcc tccgggccgg gtaggctgtc
     3241 cgggccccag tacatcaccg ccgacgccga cggcttcctg tacgtcagcg acgtcggctt
     3301 ctcccgcgtc gtcaagtatg cgcgtgacgg ctccatgctg ctttctttcg gcgccaggaa
     3361 cccgacattc gacggtctca gcctaccgac cggcgtcgcc gtcgttggcg acgtggtata
     3421 cgtcgccgac gcggcgctca aggcaatctt tttattcgac cgctacggca actacctcgg
     3481 ccgcgctcgc gtacccgggc tggtgcggcc cgaaggcctc cgggccaccg gcgacgggcg
     3541 cctgctcgtg gcagacggct ccagggtact gctcgtcgac cccgagagcg ctgcgacgat
     3601 agagctatac cggtcggagc gcaagaggcc gcgcatcctg tctgcggcgt tcgatgccaa
     3661 cggcgaactt atggcggccg acttcgacgc ctccgagatc gcctacctat cggacccggc
     3721 ctccaggttc gccgggctgt cggtcgaggt cggcagggtg tactccgact ccttccctcg
     3781 cgtcagcctc gacgttaccg tcaaggatag gcacggcagg cctgtcacgg ggctcggcta
     3841 cccgaacttc tatatctcgg aactcgtgaa gaccaaggaa cgacgcgtcg agggagataa
     3901 gcccgtcgat tatatcgctt cgtcgatacg tccggctggc gagctgtcgt tcgacggctc
     3961 gctcgaccaa tcgagcggca tcgacctgac cttcctcctc gagggttctc cccagctctc
     4021 accgatgcgg ctcgaggcga gggacgccgc ctacgccgtc tacgagtcgc tcggcggcgg
     4081 gacggattcg atggccaggc tcgtcgtggc cggccgcgtc gcccagccgc cttccgccgc
     4141 caacgtcgcg gccatagggg cggcggccct cggctacgag ccatcgtcgg actggcgctt
     4201 cgattcg
//
LOCUS       DAOPHV010000186         4194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_28738_length_4194_cov_3.162116, whole genome shotgun sequence.
ACCESSION   DAOPHV010000186 DAOPHV010000000
VERSION     DAOPHV010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28738_length_4194_cov_3.162116"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLQ29_06145"
     CDS             complement(<1..408)
                     /locus_tag="PLQ29_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86264.1"
                     /translation="MNKRDFPRIHFYDQDFVDIYDRTWAWIADYWTGSGPDSGIGGGK
                     FFFYPPERRLDQLEQSFASFFLVYSNRIYAASNGLDALYSKQEESGAIRWKYDVDSGA
                     PVLPPENPEGVSLPLFAWAEYNLYHKSANKKRVK"
     gene            631..933
                     /locus_tag="PLQ29_06150"
     CDS             631..933
                     /locus_tag="PLQ29_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86265.1"
                     /translation="MIITLYRPSAEGPIRYYTIHDRQPLLTARYSLTIAWRTGEGRER
                     EKVYGFDTLADKDKKIKQVFARKTRDGYSLLYSFIREIVSAPESIAVPESARAADA"
     gene            complement(938..2227)
                     /locus_tag="PLQ29_06155"
     CDS             complement(938..2227)
                     /locus_tag="PLQ29_06155"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPG86266.1"
                     /translation="MNGASFHSVTLDPTKCKACTTCIRFCPTEAIRVRKGRAHIADER
                     CVDCGECIRVCPNNAKKAVSDPLSALEPFAVRVALPAPTLYGQFDERFSIDRILCGLL
                     ELGFTDVFEVAEAAELVSDAAAAWLARTGDGGPWLSSACPAVVKLLQVRFPSLLDRLV
                     PIMSPMEAAARLVKERLYPGRDDVGVFFISPCAGKVTVSRYLQGLSDSSVDGVIAIKD
                     VYLPLRAALPGVTERPLHRASARGVAWAHIDGESDALAVPRAISVSGIADVIGVFEAL
                     ENGKMKDVAFIEALACPAGCVGGPLTVENPYIARARIRSMEHRATVALEPRFRAGELD
                     LRLEREVSARAGLALAPDMLKAMLLLEETELIAESLPGLDCGSCGAPSCRAFAEDVAK
                     GDAVITDCVFKLRENLRKLGEELIGMEGPQPEPGRGR"
     gene            complement(2224..2658)
                     /locus_tag="PLQ29_06160"
     CDS             complement(2224..2658)
                     /locus_tag="PLQ29_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG86267.1"
                     /translation="MKLRYEVSGEDMSLAGKASGEVKRALGRLGVNPAAIKRTAVCMY
                     EAEMNMVIHGGGGVAEAELSPDRVVVTMRDSGPGIPDLELAMQEGYSTAPEWIREMGF
                     GAGMGLPNMKRNSDELRIDSAPGKGTTVTIMVKFDSSEGTGA"
     gene            complement(2663..3022)
                     /locus_tag="PLQ29_06165"
     CDS             complement(2663..3022)
                     /locus_tag="PLQ29_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86268.1"
                     /translation="MKVRDIVSTLEGVLLTGEALLDLDIQAACGADLMSDVMAFVKEN
                     VVLLTGLVNPQSIRTADLMDIKVVVFVRGKVPPKDMVAEAERNGMAVIATKYSMFLAC
                     GRLYEAGIHGGGTRDIR"
     gene            3354..>4194
                     /locus_tag="PLQ29_06170"
     CDS             3354..>4194
                     /locus_tag="PLQ29_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024752187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPG86269.1"
                     /translation="MIINHNLSAMFANRSLGVTNSGVEKNMEKLSSGMRINRAGDDAS
                     GLAVSEKMRSQIRGLNMASKNAANGISFIQSTEGYLQETQDIIQRLRELAVQASNGIY
                     TSEDRMQIQVEVSQLVDEIDRIASHAQFNGMNVLTGRFARESGENIVTGSMWFHIGAN
                     MDQRERVYIGTMTAKGLGVKNVGDDSILKLSDPDSSNRSIGVLDSALKTVNKQRADLG
                     AYQNRLEHAVRGIDIGAENLQAAESRIRDADMASEMVEFTKNSILAQAGNAMLAQANQ
                     KTQQ"
BASE COUNT          834 a         1376 c         1268 g          716 t
ORIGIN      
        1 cttgacccgc ttcttgttgg cgctcttatg gtacaggttg tactcggccc acgcgaacaa
       61 gggcaggctg acgccctctg gattctccgg cgggagcacc ggggccccgg aatcgacgtc
      121 gtacttccag cgtatggccc cggactcttc ctgcttggag tacagtgcgt ccaggccgtt
      181 cgacgccgca tagatcctgt tcgaatagac gaggaagaac gacgcgaacg attgctcgag
      241 ctgatccagc cgccgctccg gcgggtagaa gaagaatttt ccgccgccta taccgctatc
      301 ggggccggag ccggtccaat aatccgcgat ccacgcccag gtacggtcgt agatatcgac
      361 gaagtcttgg tcgtagaaat gtatccgcgg aaaatcccgt ttattcaagg gtgacactcc
      421 cgtatcgcct ttgcgcgttt gatcactaaa cgcaaagcat ttcaagcgtt ttcaagagaa
      481 gggtgcaaat aggcagtgat agaatataat cgcggtccgc agttttgtca aattattcta
      541 taaagataat tgagatttta acagagaacg ccccgtttct ccctggagtc ccgcttgagt
      601 agttagccca tccggtcgat aatcgggtcg atgatcatca cgctgtacag gccgtccgcg
      661 gaaggcccaa tccgctatta tacgattcat gaccgtcagc cgcttctgac ggcgcgctat
      721 tcgctgacca tcgcctggag gacgggcgaa ggccgggagc gggagaaggt ctacggcttc
      781 gacaccctcg ccgacaagga caaaaagatc aagcaggtgt tcgcgaggaa gaccagggac
      841 ggctactccc ttctctacag cttcatccgc gagatcgtat ccgcgccgga atcgatcgcc
      901 gtgccggaat ccgcccgcgc agcagacgcg taaggcatta tcgcccccgg cccggctccg
      961 gctgaggccc ttccatgcct atcagctcct cgccgagctt cctgaggttc tcgcgtagct
     1021 taaagacgca gtcggtgatc acggcgtcgc ccttggccac gtcctcggcg aaggccctgc
     1081 agctcggcgc gccgcacgag ccacagtcga gccccggtag cgactcggcg atcagctcgg
     1141 tctcctcgag tagcagcatg gccttgagca tgtcgggcgc cagcgccagc ccggccctgg
     1201 ccgagacctc gcgctcaagc cgcaggtcga gctcgcccgc cctgaagcgc ggctcgagtg
     1261 ctacagtagc tcgatgttcc atcgagcgta tcctggccct cgcaatatac ggattctcca
     1321 ctgtcagcgg cccaccgaca cagccagccg ggcaggcgag cgcctcgatg aaggccacgt
     1381 ccttcatctt accgttctcg agcgcctcga agacgccaat cacgtccgca atgccgctca
     1441 cgctgatcgc cctgggcacc gcgagcgcat cgctctcgcc gtcgatatgg gcccaggcca
     1501 cgccccgggc gctcgcccta tgcagcggcc gctcggtcac gccgggcagg gccgcgcgca
     1561 gaggcaggta aacgtccttg atggctatca ccccgtcgac ggacgaatcg gacaggccct
     1621 ggaggtagcg cgaaaccgtt accttgccgg cgcacggcga tatgaagaac acgccgacgt
     1681 cgtcgcgtcc cggatagagc cgctccttga cgaggcgcgc ggcggcctcc atcggagaca
     1741 tgatcggcac gagccggtcg agcaggctcg ggaagcgcac ctgtagcagc ttgacgacgg
     1801 ccgggcaagc cgacgacagc cacggccctc cgtctccggt ccgggccagc caagccgccg
     1861 ccgcgtcgga tacgagctcg gccgcctcgg ccacctcgaa cacgtcggta aaaccaagct
     1921 cgagcaggcc gcagaggatg cgatctatgg aaaaacgctc gtcgaactga ccgtacaggg
     1981 tgggcgccgg tagcgcgacg cgcacggcga acggctccag cgccgacaag gggtcggata
     2041 cggccttctt ggcgttgttc gggcacaccc tgatgcactc gccgcagtcg acgcagcgct
     2101 cgtcggcgat atgggcccgt cccttgcgca cgcggatggc ctcggtcggg cagaaccgga
     2161 tacaggtcgt gcaggccttg cacttcgtcg ggtcgagggt aaccgagtgg aagctggccc
     2221 cgttcaagcc ccggttcctt cgctcgagtc gaatttaacc atgatcgtca cggtcgtgcc
     2281 cttaccgggc gccgagtcga tgcgtagctc gtcggaattg cgcttcatgt tcggcagccc
     2341 catgccggcg ccgaagccca tctcgcgtat ccactctgga gccgtcgaat agccctcctg
     2401 catcgccagc tcgaggtcag gaatacctgg cccggaatcc cgcatggtga cgacgacgcg
     2461 gtccgggctg agctcggcct cggccacccc gccgccgccg tggatgacca tgttcatctc
     2521 tgcctcgtac atgcagacgg cggtacgctt gatggcggcg gggttcactc cgagccggcc
     2581 gagcgcgcgc ttgacctcgc cgctcgcctt gcccgccagg gacatgtcct caccggacac
     2641 ctcgtaacgg agtttcatga gcctagcgga tatctcgcgt cccgccgccg tggatgcccg
     2701 cctcgtacag ccggccgcag gccaggaaca tcgagtactt ggtcgcgatg accgccatgc
     2761 cgttgcgctc ggcctctgcc accatatcct tgggtggcac cttgccgcgg acgaacacga
     2821 ccaccttgat gtccatcagg tcggccgtgc ggatggactg aggattgacc aacccggtca
     2881 gcagtaccac gttctccttg acgaacgcca tcacgtcgct catcaggtcg gcgccgcagg
     2941 cggcctggat gtccaggtcg agcagggcct cgccggtcaa gaggactccc tcgagggtag
     3001 aaacgatatc ccgaaccttc atgaatcctc cagcgcccgt tcgtccggcg actggacgag
     3061 tctaacacgc gccgcgaagc cggacaagcc gagagcccgc gacctctcgt cagtcggcta
     3121 cgaacggggc taaaggccgc gagccaaacg ccgatagctt taagtgtggg accgctacgg
     3181 gacggaagcg aagcgaacgc gcgatcgcct agctccgtcc acgggcgggc tctattcggt
     3241 tcacgttcac gggtgcggcc cggagcagcg taccggagaa atcgacacgt aaatttcggc
     3301 acggatgccg agacggccgt aggccgtcgc ttctatggaa aggagatcgg gttatgatca
     3361 tcaatcacaa ccttagtgcg atgttcgcca atcgctctct aggggtcacg aacagcggag
     3421 tagagaagaa catggagaag ctatcgtcgg ggatgcggat aaaccgcgcc ggcgacgacg
     3481 cttcgggact cgccgtgtct gagaagatgc gttcgcagat caggggcctc aacatggcct
     3541 ccaagaacgc cgccaacggc atctcgttca tccagagcac cgagggctac ctccaggaga
     3601 cgcaggacat aatccagcgc ctccgcgagc tcgccgtgca ggcctccaac ggtatctata
     3661 cctcggaaga ccgcatgcag atccaggtag aggtctctca gctcgtcgac gagatcgacc
     3721 gcatcgccag ccacgcccag ttcaacggca tgaacgtgct taccggccgt ttcgcccgcg
     3781 aatcgggaga gaacatcgtc accgggtcga tgtggttcca catcggcgcc aacatggatc
     3841 agcgggagcg tgtctacata ggcacgatga ccgccaaggg cctcggcgtc aagaacgtcg
     3901 gcgacgattc catcctgaag ctgtccgatc cggacagctc caacaggtcc atcggggtac
     3961 tcgattccgc cctgaaaacc gtcaacaagc agcgcgcaga cctgggagcc taccagaaca
     4021 ggctcgagca cgcggtacgc ggcatcgaca tcggcgcgga gaacctccag gccgccgaga
     4081 gccgcatccg cgacgcggac atggccagcg agatggtgga gttcaccaag aactcgatcc
     4141 tcgcgcaggc cggcaacgcg atgctcgcgc aggccaacca gaagacgcag cagg
//
LOCUS       DAOPHV010000187         4193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_28745_length_4193_cov_3.650072, whole genome shotgun sequence.
ACCESSION   DAOPHV010000187 DAOPHV010000000
VERSION     DAOPHV010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28745_length_4193_cov_3.650072"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2697)
                     /locus_tag="PLQ29_06175"
     CDS             complement(<1..2697)
                     /locus_tag="PLQ29_06175"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86270.1"
                     /translation="MVYCCIYIDYLSQLERTGVFEVTAFLDELGSTAAEFGGAVGSRK
                     DPLVVAFPSGGLFDGIQAVEALRRTVESLTVREAALRGASLTVYEGESVDDAVAFAAA
                     IRYRDHSAYSKTLSPEAASRLAAYFPSGDDGWRQPLHVGLMADADATRLFDRPRLAAT
                     LAKAIGKSRHGPRLVHLDAGSSVRSIDAIAQEMELEPERALILCGARLRPIPFSPLIE
                     AIMARFRDARGDGALVGDGALVGDDGLVGDDALLSGDDAMAFRFVEASSFSAAAPGSV
                     AMGCAAFIGSWLDAFGASGGVVACDAPERFSPEARELIAARLESGRGAERYLSIASGG
                     PVERWIGPWAARVPAGLADADDRPSALSLALGSTEGATRAALAERFGAVSGWNGDRRA
                     HGGSGLAGLLEALPLEAFIYLYTIVIAENELSADELADFVGRLGLRRAGAAVLRRLLV
                     QAGLIDPFDPRTPVEPLAPDELVAVIGDDVAETLRDGLSRYLVGLYRSGRIRASLGFL
                     RRVGERQDDERLICDCLFEDAMRPDKPLSDEPTFLSPSSAAVNRFWTALVARDRIASE
                     AAAAAADERLAGPRAQAMKALVKSELAYAQGDAERASKGAREAMLALGKGAPPRLEAR
                     SQRLMGLASLAQGRHTEAADYLTNAQELSESAGIDYERMMAAYAKAVVDFLSGAVGRA
                     LKALLHAEESSSRLFRMDAKAAVESLRGRIDLELGAYDEAARRFGALAETAAAYGLGE
                     AARRARIWRARALAYAGSFDIAAIALEAEAGTDEARPDPEAMVFRGELELLRGQPREA
                     RAWLTVPEEPPLRAFDPADSFDWSCLFSEIEGRSIGFEAADAPLSQLRTSLELFARGL
                     DERDPACAVQLHELTRSERGAGANPGMGSYGFFCYLLEERLPS"
     gene            2899..>4193
                     /locus_tag="PLQ29_06180"
     CDS             2899..>4193
                     /locus_tag="PLQ29_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPG86271.1"
                     /translation="MTINTFSQVIMSPLLSRLSVLACAAALFFSLRKKDAEGRSGSFL
                     VIIAFLVVRDTFLAVVDLPGAPYLTDLLYFSLAAFMVMAPFERSRLMLIATMAVNAAA
                     AGLYVAYAYAGVLPSLPAAAFPLVPVADALFVAASGAVNASDRGDGSSRLMSESWPVV
                     FACLGAYAAAAFFLGYESAVFVRLVVPASYAWLLVVALIDLRDHDAETIKAVSYYEES
                     IDSLYNLTFRVGTVLKGSFTTEDVLASMNEVMIAETGADGGTIYLVDEFDDIIVAKAY
                     AGTFPPPFPLPESLPRKQSRIESYLRHAQFRLGETVLGDVAKTGKNVYSPDTANDPRF
                     AALRAEGANMGEDFLRTSSFMAVPLMVEDKIIGVSAVARVGSGEQFDEAAFDRFKLLA
                     NFGTLAVSNFFSFLAANERSAIERSAGIAAEIQSTIIPK"
BASE COUNT          645 a         1463 c         1393 g          692 t
ORIGIN      
        1 cgagggcagg cgctcctcga gcaggtagca gaagaagccg tagctaccca tgcccggatt
       61 ggcgccggcg ccgcgctcgg atcgggtcag ctcgtggagc tggaccgcgc aggcaggatc
      121 gcgctcgtcg aggccccggg cgaagagctc gagcgaggtc cgtagctgcg atagcggtgc
      181 gtccgcggcc tcgaagccga tgctccgtcc ctctatctcc gagaagaggc atgaccaatc
      241 gaaggagtcc gccgggtcga aggcgcgtag cggaggctcc tccggtacgg tcaaccaggc
      301 ccgggcctcg cggggctggc cgcgaagcaa ctcgagttcg ccgcggaaga ccatggcctc
      361 gggatcgggg cgcgcctcgt cggtcccggc ctcggcctcg agcgctatgg ctgcgatgtc
      421 gaagctaccg gcgtacgcga gggccctggc tcgccagatc ctggccctcc tggccgcctc
      481 gcccaggccg tacgccgccg ccgtctcggc cagcgctccg aaacgcctgg ccgcctcgtc
      541 ataggcgccg agctcaagat ctatcctgcc gcgtagcgac tcgacggccg ccttggcgtc
      601 catcctgaac agtctcgacg acgactcctc cgcgtgtaac agggccttga gcgcccggcc
      661 gacggctccg gagaggaagt cgacgacggc cttggcgtac gcggccatca tgcgctcgta
      721 gtcgataccg gcgctttccg acagctcctg ggcgttcgtc aggtagtcgg ccgcctccgt
      781 atgccggccc tgcgctaacg acgccaggcc catcaggcgc tggctcctgg cctcgagcct
      841 cggaggcgcg cccttgccga gggccagcat ggcctccctg gcacctttcg acgcgcgctc
      901 agcgtcgccc tgggcgtagg ccagctccga cttgacgagg gccttcatcg cctgggcccg
      961 cggaccggcg agccgttcgt cggccgcggc ggcggccgcc tcgctggcta tccggtcgcg
     1021 ggcgacgagg gccgtccaga atctgttgac ggccgcggac gacggggaca ggaaagtggg
     1081 ctcatcgctc aggggtttgt ccggcctcat cgcgtcctcg aacaggcagt cgcagatcag
     1141 acgctcgtcg tcctggcgct ctccgaccct gcggaggaag ccgaggctgg ctcggatgcg
     1201 gcccgaacga tagagcccga ccaggtagcg cgataggccg tcccgcagcg tctcggccac
     1261 gtcgtccccg atgacggcga ccagctcgtc gggcgctagc ggttctaccg gcgtcctcgg
     1321 atcgaacggg tcgatgaggc cggcctggac gagtaagcgg cgcagaacgg cggcgcccgc
     1381 gcgcctgagc ccgaggcggc cgacgaagtc ggcgagttca tccgctgaca gctcgttctc
     1441 ggcgatgacg atcgtataca ggtagatgaa cgcctcgagc ggcagggcct cgagcaggcc
     1501 cgcgagtccg gagcccccat gggctcgacg gtcgccgttc cagccggata cggcgccgaa
     1561 gcgttcggcg agcgccgcgc gcgtcgcgcc ctcggtcgag ccgagcgcca aggacagggc
     1621 gctcggtcta tcgtcggcgt cggcgaggcc ggccggtacc ctggccgccc aggggcctat
     1681 ccagcgctcg accggtccgc cagatgctat ggacaggtag cgctcggcgc cgcggccgga
     1741 ctccagcctc gccgcgatga gctcgcgggc ctcgggcgag aaacgttcgg gagcgtcgca
     1801 ggcgacgact ccgcccgagg caccgaaggc gtcgagccag gagcctatga acgcggcgca
     1861 tcccatggct accgaaccgg gagccgcagc cgagaacgag gacgcctcga cgaagcggaa
     1921 ggccatggcg tcgtcgccgc ttaataacgc gtcgtcgcct actaacccgt cgtcgcctac
     1981 taacgcgccg tcgcctacta acgcgccgtc gccgcgcgcg tctcggaagc gcgccatgat
     2041 cgcctcgatc aatggcgaga acggtatggg gcgtagcctg gcgccgcaga gtatcagcgc
     2101 gcgctccggc tccagctcca tctcctgagc tatcgcgtcg atagaccgga cgctcgagcc
     2161 ggcgtcgagg tggacgagcc tcggtccgtg cctcgacttg ccgatggcct tggccagcgt
     2221 ggccgcgagc ctgggccggt cgaacaggcg ggtcgcgtcg gcgtcggcca tcaggccgac
     2281 gtggagcggt tgccgccagc catcgtcgcc gctcgggaaa taggccgcca ggcggctcgc
     2341 cgcctcgggg ctgagcgtct tcgagtacgc cgaatggtcg cgatagcgaa tcgccgccgc
     2401 gaaggccacg gcatcgtcga ccgattcgcc ctcgtagacg gtcaggctcg ccccgcgtag
     2461 cgcggcttcg cggaccgtca gcgactcgac cgtcctccgc agggcctcga ctgcctggat
     2521 accgtcgaag agaccgccgg aggggaaggc gacgacgagc ggatccttcc tggagccgac
     2581 cgcgccgccg aattcggccg ccgtcgaccc gagttcgtcc aggaaggccg tgacctcgaa
     2641 cacgccggta cgctcgagct gagacaggta atctatgtag atgcagcagt acaccatgag
     2701 gcgaacgtcc cccgtgcgtt tttctctcag gcgagtatag tccatgccgg gccgatcggg
     2761 aagcgggatt cgaggctgcc gggcttccag ctttcctttt cgcttgacga tatttttttt
     2821 gccatgctac acttgcgccc tagcgggtac gagcggtccc gcgacctttc ggtccgagca
     2881 taacctcaag ggaatcctat gactatcaat acgttcagcc aggttatcat gtcgccgctc
     2941 ctgtcccggc tatccgtgct ggcgtgcgcg gccgcgttgt tcttctcgct acgcaagaag
     3001 gacgccgagg ggcgctccgg ctcgttcctc gtcatcatag ccttcctcgt cgtcagggac
     3061 accttcctgg ccgtcgtcga cctaccgggc gcgccgtacc tgaccgacct cctctatttc
     3121 agcctggcgg cgttcatggt catggcgccg ttcgagcgat cgaggctcat gctgatcgcg
     3181 accatggccg tcaacgcggc cgcggccgga ctctacgtcg cctacgccta tgccggcgtc
     3241 ctgccgtcgt taccggccgc cgccttccct ctggtgcccg tggccgacgc tctgttcgtc
     3301 gccgcgtcgg gtgccgtcaa cgcctccgac cgtggagacg gatccagtcg gctgatgtcc
     3361 gagagctggc cggtcgtctt cgcctgtctc ggcgcgtacg ccgccgccgc gttcttcctc
     3421 ggctacgagt cggcggtgtt cgttcggctc gtggtgcccg cgtcgtacgc ctggctgctc
     3481 gtcgtcgccc tcatcgacct gcgcgatcac gacgccgaga cgatcaaggc cgtctcctac
     3541 tacgaggagt ccatcgactc gctgtataac ctgaccttcc gcgtcggtac cgtgctcaaa
     3601 ggcagcttca cgacggagga cgtgctcgcc agcatgaacg aggtcatgat cgccgagacc
     3661 ggggccgatg gcggcacgat ctacctcgtg gacgaattcg acgatatcat cgtagccaag
     3721 gcctacgccg gcaccttccc gccgcccttc ccgctacccg agagcctgcc gcgcaagcag
     3781 agccggatcg agtcgtacct gcggcacgcc cagttcaggc tcggcgaaac cgtgctcggc
     3841 gacgtggcca agaccggcaa gaacgtctac tcgccggaca cggcgaacga cccgcgcttc
     3901 gcggcgttac gggccgaggg agcgaacatg ggcgaggatt tcctcaggac ctcctcgttc
     3961 atggccgtgc cgctgatggt ggaggacaag atcatcggcg tgtcggccgt cgcccgggtg
     4021 ggttcgggcg agcagttcga cgaggcggcc ttcgaccgct tcaagctgct ggccaatttc
     4081 ggcaccctgg ccgtcagtaa cttcttctcg ttcctggccg ccaacgagcg tagcgcgatc
     4141 gagcgttctg ccggcatcgc ggccgagatc cagagcacca tcatcccgaa gaa
//
LOCUS       DAOPHV010000188         4176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_28911_length_4176_cov_2.837903, whole genome shotgun sequence.
ACCESSION   DAOPHV010000188 DAOPHV010000000
VERSION     DAOPHV010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28911_length_4176_cov_2.837903"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLQ29_06185"
     CDS             <1..295
                     /locus_tag="PLQ29_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HPG86272.1"
                     /translation="NYGGRDEILRAVSKLPDGVRAYSAEEFRACLDLPDFPDPDLIIR
                     AGGELRLSNFLLWQAAYSELYFSDVLWPDWNATHLEAALAEYARRERRYGDAR"
     gene            292..1164
                     /locus_tag="PLQ29_06190"
     CDS             292..1164
                     /locus_tag="PLQ29_06190"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPG86273.1"
                     /translation="MSNFSTRILFVGAAIPALFALAAYVPFMDHLPLAILVLAFAAGS
                     GVELRRIVEPSAAKPRTVLAALFAALPSAVVYAVRQARPEAGLAASWLAPVAGVSIGL
                     FLISALPIALPRKADNVRASVAIATANAFYLLYPGALASTIIVILGSTAESGLLLIWF
                     ATVVFGNDSLAWLAGVTLGHKRGIFTVSPNKSLEGLLAGMAGSVGFAFLGPVLLPGIV
                     PRSWPTLLALGLACGVAVVAGDLFESAIKRSAGVKDSGSIVPGRGGILDSFDSLLFAA
                     PVFIGVLAAAGVAL"
     gene            1184..2320
                     /gene="dxr"
                     /locus_tag="PLQ29_06195"
     CDS             1184..2320
                     /gene="dxr"
                     /locus_tag="PLQ29_06195"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPG86274.1"
                     /translation="MISIRRVIIIGATGSIGRQAIDVVARHPDRFSVVGLSAHSDEDG
                     LVAASCHFPGAALCLSSRSPRSGAIAFSGIGGLSALVRDTDADIVLNAASGAAGLSVS
                     FDALSSGKDLALANKETVVMAGRLAFAAARRASRLILPVDSEHSAIFNMIERFGRETV
                     RGVVITASGGAFRDEPLESLPGKKPTDALAHPTWSMGAKITVDSATMANKGLEVIEAA
                     RLFGLTGAEIDVVLHPESRVHSFIRARDGSLYAQMSRPDMRLPILNALAWPESVDESV
                     ADMDPCDVPLRFSRPDPERYPLLRTAYDALASGEGSTDAYNAANEVAVAAFMDGAIRF
                     TDIARVVSRTLERAYPPTLEGLEAVLAVDADARRVARSIVKEIS"
     gene            2317..3675
                     /gene="rseP"
                     /locus_tag="PLQ29_06200"
     CDS             2317..3675
                     /gene="rseP"
                     /locus_tag="PLQ29_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPG86275.1"
                     /translation="MTIATIALGLVGLGFVVFFHELGHFIMARLVGVEVEEFSIGMGP
                     RLLSRRFGKTAYTLSSLPIGGYCRMKGEDSFKKAIEQGLNEFPRESGSYFGAAPLKRI
                     LIALGGPFANVVLAFILYAAIAGAGYETRSWENRIVLASDTDGSSYPADRAGLASGDR
                     IVAIGGEAVSSFSEIQEAIALSARKPIPLTVDRHGESFSLVVEPELDAGSGAGRVGVY
                     PWIEPVVETVAPDGAAALAGLRPGDRIVAINGEPAIHGVAIASRLEADRPAKITVAYE
                     RGGFIAETTVVLAYSEERGADLGVGWKSTVSKIQAESLGQAMVMGVDETADTISATYR
                     GIASLFMGVNLLQAVSGPARITWMVGTVAKSGLTDGTVGGFSTLLGFLAILSIGLFAM
                     NLLPIPLLDGGSIALFVVELVRRKAPKVKTVLRYQTIGLVAVAALFLLSTIGDVLFFS
                     GR"
     gene            3710..>4176
                     /locus_tag="PLQ29_06205"
     CDS             3710..>4176
                     /locus_tag="PLQ29_06205"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HPG86276.1"
                     /translation="MKKIICRCDAEVEVDAPDLLDLDADPAMLGRLARGDSPSAACPR
                     CGAIVRAELPLKVRSASRGVDIVVLPEIERLATYRGKADPTGSAEILLGYQELFERSR
                     MLSDGLDPSCVEALKYALQGKAEETEPKAEISVFYNGVSGGSLFVAEAVEDAEL"
BASE COUNT          652 a         1388 c         1359 g          777 t
ORIGIN      
        1 caactacggc ggccgcgacg agatcctccg ggccgtttcg aagctcccgg acggggtgcg
       61 cgcgtatagc gccgaagaat tccgcgcctg tctcgacctg cccgacttcc ctgacccgga
      121 tctgataatc agggctgggg gcgagctacg cctttccaat ttcctactct ggcaggccgc
      181 atattcggag ctgtacttct ccgacgtgct gtggcccgac tggaacgcta cccatctaga
      241 agcggctctc gccgagtacg cccgcaggga gcgcagatac ggtgacgctc gatgagtaac
      301 ttctctaccc gcatactgtt cgtcggcgcg gccatccccg cgctgttcgc cctggccgcc
      361 tacgttccgt tcatggatca cctgccgctg gccatcctcg tactggcttt cgcggcgggc
      421 tcaggcgtcg agctgcggcg catcgtggag ccatcggccg ccaagcccag gaccgtcctg
      481 gcggcgctgt tcgccgccct gccaagcgcg gtcgtctacg ccgtgaggca ggcccgtccc
      541 gaggctggcc tggccgcctc gtggctcgcg ccggtggccg gcgtctcgat aggcttgttc
      601 ctgatctcag ccctgcccat cgcgctgccg aggaaggccg ataatgtacg cgcctcggta
      661 gcgatagcca cggccaacgc gttctatctg ctgtatcccg gcgccctcgc ctcgaccatc
      721 atcgtcatac tcggctccac ggcggagtcc ggcctccttt taatatggtt cgctaccgtc
      781 gtcttcggga acgatagcct ggcttggctg gccggcgtga ccctcggcca caagcgcggc
      841 atcttcaccg tgtccccgaa caagagcctc gaggggctcc tggccgggat ggccggcagc
      901 gtcgggttcg cgttccttgg cccggtcctg ctaccgggaa tcgtgcctcg ttcctggccg
      961 acgctcctgg ccctcggcct ggcctgcggc gtcgcggtcg tcgccggcga cctgttcgaa
     1021 tcggcgatca agcggagcgc gggcgtcaag gattcgggct ccatcgtccc cgggcgaggc
     1081 gggatactcg actcgttcga cagcctgcta ttcgccgcgc cggttttcat aggcgtattg
     1141 gccgccgccg gcgtagcgct gtgatacgcc gggaaggacg ggcgtgatct ctatccgacg
     1201 cgtcatcatc ataggcgcga ccggctccat agggcggcag gcgatcgacg tcgtcgcccg
     1261 acaccccgac cgcttctccg tggtcggact gtcggcccat tccgacgagg acggcctcgt
     1321 ggcggcctcg tgccatttcc ccggcgctgc cttatgcctg agctcgcggt cgcccaggtc
     1381 gggggccata gccttcagcg gaatcggtgg cctaagcgcc ctcgtccgcg ataccgacgc
     1441 ggatatagtc ttgaacgccg cctcaggcgc cgccgggctg tcggtatcct tcgacgccct
     1501 ctcgtccggc aaggatctcg cgctagccaa caaggagacg gtcgtgatgg ccggccggct
     1561 cgccttcgcc gccgcccgtc gggccagccg cctgatcctc ccggtcgact cggagcattc
     1621 ggccatcttc aatatgatcg agcgcttcgg tcgggaaacc gtacgcgggg tcgtcatcac
     1681 ggcgtcggga ggcgccttcc gcgacgagcc gctcgagtcg ctacccggca agaagcccac
     1741 ggacgccctc gcccacccta cctggagcat gggcgcaaag atcaccgtcg attcggcgac
     1801 gatggcgaat aagggcctcg aggtgatcga ggccgcgagg ctgttcggcc tgacgggagc
     1861 ggagatcgat gtggtgttgc atcccgaaag ccgcgtccac tcgttcatcc gtgccaggga
     1921 tggttccctc tacgcccaga tgtcgaggcc cgatatgcgc ctgcctatcc tgaacgcgct
     1981 cgcttggccc gaatcggtcg acgaatcggt cgccgacatg gacccctgcg acgttcccct
     2041 gcgtttctcg cgccccgatc ccgagcgcta ccccctgctc cgtacggcct acgacgctct
     2101 cgcctccggc gagggctcga ccgacgccta caacgccgcg aacgaagtcg cggtcgcggc
     2161 gttcatggac ggcgcgatac gctttaccga cattgcccgc gtcgtctcgc ggaccctgga
     2221 gcgcgcgtat ccgccgacgc tcgaagggct cgaggcggtg ctggccgtcg acgccgacgc
     2281 caggcgcgtc gcccgatcga tagtcaagga gatctcatga ccatcgccac catagcgctc
     2341 ggcctcgtcg ggctcggctt cgtcgtattc ttccatgagc tggggcattt tataatggcc
     2401 aggctcgtcg gcgtcgaagt cgaagaattc tccatcggaa tggggccacg gctcctctcc
     2461 aggcgctttg gcaagacggc gtacaccctg tcgtccctgc cgattggcgg ttattgtaga
     2521 atgaagggcg aagactcgtt caagaaggcc atcgaacagg gtctgaacga gttcccccgc
     2581 gagagcggca gctacttcgg cgccgcgccg ctcaagcgca tcctcatagc gctcggcggg
     2641 cccttcgcca acgtcgtcct ggctttcatc ctctacgcgg ccatagccgg agccggttac
     2701 gagacgcgta gctgggagaa tcgcatcgta ctggcgagcg acaccgacgg cagctcgtat
     2761 ccggcggacc gggccgggct ggcgagcggt gacaggatag tcgcgatcgg cggcgaagcc
     2821 gtatcgtcgt tctcggagat ccaggaggcc atcgcgctgt ccgccaggaa gccgataccg
     2881 ttgacggtcg atcgccatgg cgagtcgttc agcctggtcg tcgagcctga gctcgacgct
     2941 ggctcgggcg ctggcagggt cggcgtgtat ccatggatag agcccgtggt cgagacggtc
     3001 gcgcctgacg gggccgccgc cctcgccggc ctgaggccgg gcgacaggat cgtggctata
     3061 aacggcgagc cggctatcca cggggtcgcg atagcctcgc ggctcgaagc cgatagaccg
     3121 gccaagataa cggtagccta cgagcgcggc ggtttcatcg ccgagacgac cgtagtgctg
     3181 gcgtactcgg aggagcgcgg cgccgacttg ggcgtcggct ggaagtcgac cgtctccaag
     3241 atacaggcgg agagcctcgg ccaggctatg gtcatgggcg tcgacgagac ggctgatacc
     3301 ataagcgcga cctatcgagg cattgcgtcg ctattcatgg gcgtgaacct gctccaagca
     3361 gtctcgggtc cggcgcgtat tacctggatg gtcggcacgg tggccaagag tgggctgact
     3421 gatggaacgg ttggcggttt ctcgacgctg ctgggcttcc tggccatact gtcgatcggc
     3481 ttgttcgcta tgaatctgct acccattccg ctgctcgacg gcggttcgat cgccctgttc
     3541 gtcgtcgagc tcgtcaggcg caaggcgccc aaggtcaaga cggtgctcag gtaccagacc
     3601 atcggcctgg tcgccgtcgc ggccctgttc ctactatcga cgataggcga cgtcctgttc
     3661 ttctccggac gttagcgata taccctatag tattgtgacg gagggtgcca tgaagaagat
     3721 aatctgccgc tgcgacgccg aggtcgaggt ggacgcgccc gatctgctcg acctcgacgc
     3781 ggatccggcc atgctggggc gactggcgcg cggcgactcg ccttcggccg cgtgtccacg
     3841 ctgcggcgcg atcgtcaggg cggagctgcc tttgaaggtg cggtcggcct cgcgcggcgt
     3901 agatatcgtc gtgctacccg agattgaacg tttggccacc tatcgcggca aggccgaccc
     3961 aaccggaagc gccgagatac tgctcggcta tcaagagctg ttcgagcgct ccaggatgct
     4021 gagcgacggc ctcgacccca gctgcgtcga ggcgctcaag tacgcgcttc agggcaaggc
     4081 cgaggagacg gagccgaagg ccgagatcag cgtgttctat aacggggtat cgggcggctc
     4141 gctcttcgtc gctgaggccg tcgaagatgc cgaact
//
LOCUS       DAOPHV010000189         4161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_29059_length_4161_cov_3.870434, whole genome shotgun sequence.
ACCESSION   DAOPHV010000189 DAOPHV010000000
VERSION     DAOPHV010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29059_length_4161_cov_3.870434"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1729)
                     /locus_tag="PLQ29_06210"
     CDS             complement(53..1729)
                     /locus_tag="PLQ29_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HPG86277.1"
                     /translation="MRLRRSIYAPIAFFLAAAGASAQVPAKSAEPLKVASAISAVTVF
                     SDRALVRRTAELRVQAGETTLVFTDLPAAIDPASLQVSGSGLFTLRDVRVTTRQLSRD
                     VSAERKALEDERRAYEASIAIESDRVREAEAERTFVDNMAKRVTSAASEDEKAPLDTA
                     AWARMLDFYRGRYQALNLAVREAKASITTLQAEVDRVNRELRALGGGSGLSVVEAELS
                     LEAQAPTTARLVLSYLVSGPSWMPDYVVRADSAGGKLSLHYRATVRQGTGEDWSGVAL
                     SLSTARPQVGGSAPELEPWYIDVYKPPVSYRASAKESKAMAAPAPAAVAQDELFEAAA
                     EAPAMGYAGSVASVGATAVTFTVPGATIVASDNRERTVTVAMLELPVSYSYAAVPKLS
                     PHAYFRAEATNDSDYPLLAGRSHVYVDGGYVADSAIGAVPPGGSFSADLGVDEAVSVE
                     RKLVKKFDETTGVVSKKQKTTWQYAISVKNGKKAAIRLVVTDQLPISANEQIVVKGLE
                     PAWSKDTATLAKVEGERFVWTLELGAGQEARLPLSFSVEYPKGTPIVGLE"
     gene            1930..>4161
                     /locus_tag="PLQ29_06215"
     CDS             1930..>4161
                     /locus_tag="PLQ29_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPG86278.1"
                     /translation="MASLGINIGSSSLKLVLAEGDTIVWSASTPHEGDFHSAVRKLLS
                     EKAIPLGTKVLVTGNEGRYLFNAAGTLEPLCVEAALKASGLKADAVASMGGEDLVVYS
                     LDQNGKIVNNFSGNKCASGTGEFLKQQLARMDMTLADIAKVPDSARVLALSTRCSVFM
                     KSDCTHRLNKREATKDDIVLSLSNVMAGKVVDFLKRAKITDGRVILTGGLTLNKHMLR
                     FIKERAPGIDFIVPDSASVFEAYGAAVLAESAGSPLPSTESLLKPNVVRFDTLPDLKK
                     WRDKVRFFEKGEGKVVAGRKYVLGVDGGSTTTKACLVDAETDEIVASHYGRTHGDPVK
                     ALKECLAVIRDKIIADTGDAKVDIGLTATTGSSREILGVFLETPGVYNEIIAHAVGTA
                     YFDKGVETIFEIGGQDAKYVLLKNGVPIDYAMNEACSAGTGSFLEESSSGDLSIHSAK
                     DIAPIALAADAPLKFGEHCSAFINSDIRKAVQQGATRENITAGIVVSIVANYLNRVVG
                     NRTIGGKVFLQGGVAKNEAVPLAFAMMLDKEILVPPSPELMGCFGVALLAKRKRAEGL
                     LDDTVVDLEAAINRDIVYERVFTCKSCENNCPIQVLKVNGHPYMFGGRCNKYTNTRKA
                     LKDVPVFDYVEKRQKLLYETFAAPADAIVARRDYTVGIPRAFSTHSLYPLYSWFFHEL
                     GIKTVLSTEVAQQGVVRVESAYCFPAEIAHGAVQDCLDKGVDYVLLPHFRDMASYEDD
                     VHAN"
BASE COUNT          761 a         1410 c         1287 g          703 t
ORIGIN      
        1 ccggcccgac gagatcgtcg gatggaagga cggcaacgac ccttggatct gactactcga
       61 ggccgacgat aggcgtgccc ttcgggtact ccacgctgaa tgaaaggggt agtctcgcct
      121 cctggccggc tcccaactcg agcgtccaga cgaagcgctc gccctccacc ttggcgagag
      181 tcgccgtatc cttgctccac gccggctcga ggcccttgac gacgatctgc tcgttcgccg
      241 agatgggcag ctgatcggtg acgacgagcc tgatggcggc cttcttgccg ttcttgacgc
      301 ttatggcgta ctgccaggtg gtcttctgct tcttcgagac cacgccggtg gtctcgtcga
      361 acttcttgac gagcttgcgt tcgaccgaga cggcttcgtc gacacccaga tcggcgctga
      421 agctcccacc cggtggaacg gctcctatgg ccgagtcggc cacgtaaccg ccgtccacgt
      481 agacatggga ccgccccgcg agtagcgggt agtccgagtc gttcgtcgcc tcggccctga
      541 agtaggcatg aggcgacagc ttaggcaccg ccgcgtacga gtagctgacc ggtagctcga
      601 gcatcgcgac ggtaacggtc cgctcgcggt tgtccgacgc gacgatcgtc gccccgggca
      661 ccgtgaaggt caccgccgta gctccgaccg aggctacgga accggcgtag cccatggccg
      721 gagcctcggc ggccgcctcg aagagctcgt cctgggccac ggcggccgga gccggagcgg
      781 ccatcgcctt cgactccttg gccgacgcgc ggtaggagac cggcggcttg tacacgtcga
      841 tgtaccatgg ctcgagctcg ggcgccgagc cgcccacctg gggcctggcc gtcgaaaggc
      901 tgagcgccac gcccgaccag tcctcaccgg tgccttgcct gaccgtcgcc cgatagtgga
      961 gcgacagctt gccgccagcc gaatcggctc gcacgacgta atcgggcatc caggacggcc
     1021 ccgagactag gtatgacagt acgagccgag ccgtcgtagg cgcctgcgcc tcgagcgaca
     1081 gctccgcctc gacgacggaa aggcccgagc caccgccgag cgcccgcagc tcgcggttca
     1141 cccggtcgac ctcggcctgc agggtggtta tgctcgcctt ggcctcgcgt accgccaggt
     1201 tcagggcttg gtagcggcca cgatagaagt cgagcatcct ggcccaggcc gccgtatcga
     1261 gcggcgcctt ctcgtcttcg ctcgccgcgc tcgtgacgcg cttggccata ttgtcgacga
     1321 acgttcgttc ggcctccgcc tcgcgcacgc ggtccgactc gatcgctatc gaggcctcgt
     1381 aggctcgacg ctcgtcctcg agcgccttgc gctcggcgga aacgtcgcga gacagctgcc
     1441 tcgtagttac ccgtacgtcg cgtagcgtga acaaaccgga gccgctcacc tgaaggctgg
     1501 cggggtcaat cgccgcaggc aggtcggtga agacgagcgt cgtctcaccg gcctgaacgc
     1561 gtagttcggc tgtccgcctg acgagcgcgc ggtccgagaa cacggtaacg gcgcttatgg
     1621 ccgaggcgac cttgagcggc tcggcggact tggcgggtac ctgggcgctc gccccggccg
     1681 cagccaggaa gaacgcgatc ggggcgtata tgctcctacg tagtctcacg gatagacctc
     1741 cattcgatac ctctagtgta acgcgtcgga caggttttct gtagtgcggg ctcgccgtcg
     1801 gcttggtcgt accagctgac agtattgtaa tgaatgtctt gcaagtatat gatcttaaca
     1861 ctccagggct cccggacacg gcgtggcgcc tggaaatctg cgacaaaagt cccggtgagg
     1921 ttctcgtcta tggcatcatt aggcatcaac attggctcct cgagtctcaa gctcgtgctc
     1981 gccgaaggcg atacgatagt ctggtcggcg tcgacgcccc acgagggcga tttccattcc
     2041 gccgttcgga aactgctctc cgagaaggcc atccccctcg ggacgaaagt cctggtaacc
     2101 ggcaacgaag gccgctacct gttcaacgcc gccggcaccc tcgagccgct ctgcgtcgag
     2161 gccgcgctca aggcgtcggg tctcaaggcg gacgccgtcg cgagcatggg cggcgaggac
     2221 ctcgtcgtct actcgctcga ccagaacggc aagatagtca acaacttctc gggcaataaa
     2281 tgcgcctcgg gcaccggcga gtttctcaag cagcagctgg cccgcatgga catgacgctc
     2341 gccgacatcg cgaaggtgcc agatagcgcc agggttctcg cgctgtccac gcgctgctcg
     2401 gtgttcatga agagcgactg cacccacagg ctgaacaagc gcgaggccac caaggacgac
     2461 atcgtgttgt cgctgtcgaa cgtgatggcc ggcaaggtcg tcgacttcct caagcgggca
     2521 aagatcaccg acggcagggt catcctgacc ggcggcctga cgctgaacaa gcacatgctg
     2581 cgcttcatca aggagcgggc gcccggtatc gacttcatcg tgcctgatag cgcctcggtc
     2641 ttcgaggcct acggcgccgc ggtgctcgcc gagagcgccg gctccccgct gccgtcgacc
     2701 gagtccctgc tcaagcccaa cgtggtgcgc ttcgacaccc tccccgacct caagaagtgg
     2761 cgcgacaagg tgcgcttctt cgagaagggc gagggcaagg tcgtcgccgg caggaagtac
     2821 gtactcggcg tggacggcgg ctcgacgacg acgaaagcct gcctcgtcga cgcggagacc
     2881 gacgagatcg tcgcgagcca ctacggccgc acccacggcg acccggtcaa ggccctcaag
     2941 gagtgcctcg ccgtcatccg cgacaagatc atcgccgata cgggcgacgc caaggtcgac
     3001 atcggactga ccgccacgac tggaagctcc cgcgagatac tcggcgtgtt cctcgagacg
     3061 cccggcgtgt acaacgagat catcgcccac gccgtcggca ccgcctactt cgacaagggt
     3121 gtcgagacca tcttcgagat cggaggccag gacgccaagt atgtgctgct caagaacggc
     3181 gtaccgatcg actacgcgat gaacgaggcc tgttcggcgg gcaccggtag cttcctcgag
     3241 gagtcgtcgt ccggcgacct ctccatccac agcgccaagg acatcgcgcc gatcgcgctg
     3301 gccgcggacg cgccgctcaa gttcggcgag cactgctcgg ccttcatcaa ctcggacatc
     3361 cgcaaggccg ttcagcaggg agcgacgcga gagaatatca ccgccggcat cgtcgtatcc
     3421 atcgtcgcca attacctgaa ccgcgtcgtc ggcaaccgca cgataggcgg caaggtcttc
     3481 ctgcagggcg gtgtggccaa gaacgaagcc gtaccgctag ccttcgcgat gatgctcgac
     3541 aaggagatcc tcgtcccgcc gtcgcccgag ctcatgggct gcttcggcgt cgcgctgctc
     3601 gccaagcgca agcgcgccga ggggctcctc gacgatacgg tcgtcgacct cgaggccgcg
     3661 atcaaccgcg acatcgtcta tgaacgcgtc ttcacctgca agtcctgtga gaacaactgc
     3721 ccgatccagg tcctcaaggt caacgggcac ccctatatgt tcggcggacg atgcaacaag
     3781 tacacgaaca cccgcaaggc gctcaaagac gtaccggtct tcgactacgt cgagaaaagg
     3841 cagaagctac tctacgagac cttcgcggca cccgccgacg ccatcgtcgc caggcgcgac
     3901 tacacagtcg gcatcccgcg cgccttctcg acgcacagcc tctatcctct ctactcctgg
     3961 ttcttccacg agctcggcat caaaaccgta ctgtccaccg aagtcgccca gcagggagtc
     4021 gtccgcgtcg aaagcgcgta ctgcttcccg gcggagatcg cccacggcgc ggtgcaggac
     4081 tgcctggaca agggcgtcga ctacgtgctc ctgccccact tccgcgacat ggcctcctac
     4141 gaggacgacg tgcacgcgaa c
//
LOCUS       DAOPHV010000190         4127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_29369_length_4127_cov_3.973232, whole genome shotgun sequence.
ACCESSION   DAOPHV010000190 DAOPHV010000000
VERSION     DAOPHV010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29369_length_4127_cov_3.973232"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="PLQ29_06220"
     CDS             complement(<1..1238)
                     /locus_tag="PLQ29_06220"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86279.1"
                     /translation="MNSLRLETLSKRLAGHPPLAEALRRIDEGSFPVDCSELEQPLAA
                     LMTAEAARMSGGSRRYFVVTPTDAEAEAFVDDCRAAGAEAMVFPWWSAAAYRPVEPRS
                     AAFGQRTAALAAVLDDHGPRVIVMGQRAAMTPVPPRSAFERGLFRVRKGDEIDAGALA
                     DRLAAWGYLRVPRVSLSGEFALRGEVLDVFMPGDESALRIVFEYDEVEEIRRFEPVSQ
                     ASDPERPDTVLLRPSRECVWTPELAARVGEALAACPGPSGEDRSPAIASVVESLTELG
                     EAKGEELLYPLAYGGPSSLFGYLGPDDVVVLLERERLLGQSEAVKREYGGLYRKALLE
                     GPVPPPEALLVDFEREAAALSRVLSSWSLKDESAGDRLAFGSEPPRSFFGNVNYFRDE
                     LDSLLRAGYERRRFDARSLA"
     gene            1321..2262
                     /locus_tag="PLQ29_06225"
     CDS             1321..2262
                     /locus_tag="PLQ29_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86280.1"
                     /translation="MHPNRGVLAALVAALCLGAAAAQAELTISFFDKAVYTPGSDVYV
                     KVTVRNDSPSTWRFKLADEKRLSVGFEVRSLANRRLEASDSWKRAMSSSAPVYYRELA
                     IQPGEEYSFVENVADYVALSEPGAFFVVCTLYPELSGRTAESSTIRSNALSLSIRPGA
                     PTPAVAERFVAGTAEILKAERIGPDEVVSRTIRARQKGRWNEFFLYLDVERLLKANSD
                     KSRSYDRESDDGRRRMIESYKADLMASVVDADIVVVPSSFDILETRYGASYGTVRVTQ
                     KFDYDGFKMVKEYTYELERRDDIWYIVAYTVRNKGTE"
     gene            2259..2990
                     /locus_tag="PLQ29_06230"
     CDS             2259..2990
                     /locus_tag="PLQ29_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPG86281.1"
                     /translation="MKALIVVQDDAVAEVVSFYLHPLGLDIIHYRDPLKALDNLDEIM
                     PDAVIMSARDFPRHWKAIVVGLRSARPKSACAVILLKGDYFPFEEAAKAAYLGVNGVV
                     KEDLSDKAELDRFQQLLKRYVEVDDARVSDRHAPGPWDRLGFIFCHPVTYQPIAGAIE
                     TISSSGLSFIPETNAAVSDMEPGLVLEEASLRLDRDILSLRCKLIRNGPVMAFSFEGC
                     EPERDAIADYIAGRAEREIKALLNH"
     gene            3015..3311
                     /locus_tag="PLQ29_06235"
     CDS             3015..3311
                     /locus_tag="PLQ29_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPG86282.1"
                     /translation="MNVNVPCDQQRIADFARKLKAVGHPIRLRLLCLIAREQDPCVSD
                     LWQCLEQPQPVISQHLAILKQAGIVSAEVQKTKRVYSISDPFISDMIRKMVTEA"
     gene            complement(3324..>4127)
                     /locus_tag="PLQ29_06240"
     CDS             complement(3324..>4127)
                     /locus_tag="PLQ29_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86283.1"
                     /translation="LVEVLLLVIEYRSLGELGDSIERLRECVRLLALYPEAEYWKGRL
                     FFIEGELALAELQYERAYGMREALEVPGEAYSILYAMAELYEARSEYVAWENVMLRVI
                     EGLDSGVDRYLEEAMLATLTEQGFNRFMTLYRLDDSYSKSAHASLAAFYLERGRAAAP
                     RHAAIAVNMILSRAIAMLKARDGDYVWTDLQNFMARALGRRDVMAYLDETGFAANLFT
                     LADALYVAGARAPALMLWGVIADQGIAPLSARAGARLSDPRSAVRRGQP"
BASE COUNT          770 a         1403 c         1306 g          648 t
ORIGIN      
        1 aaggcgaggc tgcgggcgtc gaaccgccgc cgctcgtagc cggcccgcag cagcgagtcg
       61 agctcgtcgc ggaagtagtt gacgttgccg aagaaggacc ggggcggctc ggagccgaag
      121 gcgagccggt cgcccgccga ctcgtccttg agcgaccagc tcgagaggac ccgggacagc
      181 gcggccgcct cgcgctcgaa gtcgacaagc agggcttcgg gcggcggcac cggtccctcg
      241 agcagggcct tgcggtacag gccgccgtac tcgcgcttga ccgcctcgct ctgacccaag
      301 aggcgctccc gctcgagcag cacgacgacg tcgtctggcc cgagatagcc gaacaggctc
      361 gacgggccgc cgtaggcgag cgggtacagg agctcctcgc ccttggcctc gccgagctcg
      421 gtcagcgact cgacgacgga cgctatggcc ggcgagcggt cctcgcccga ggggccggga
      481 caggccgcga gcgcctcgcc gacgcgggcc gccagctccg gcgtccagac gcactcccgc
      541 gacggcctga gcagcaccgt atcgggacgc tccgggtcgg aggcctgcga caccggctcg
      601 aacctgcgaa tctcctcgac ctcgtcgtac tcgaagacta tcctgagcgc gctctcgtcg
      661 cccggcatga agacatcgag cacctcgccg cgcaaggcga actcgccgga aaggctcacg
      721 cgcggtacgc gcaggtagcc ccaggccgca agccggtcgg cgagcgcgcc ggcatctatc
      781 tcgtcgccct tgcgtacccg aaaaaggcct cgctcgaagg ccgagcgagg cggcaccggc
      841 gtcatggcgg cgcgctggcc catgacgatg acgcgcggac cgtggtcgtc aagcaccgcc
      901 gcgagggccg ccgtccgttg gccgaaggcc gccgagcgcg gctcgaccgg ccggtaggcc
      961 gcggcgctcc accaagggaa caccatcgcc tcggcgccgg cggcgcggca gtcgtcgacg
     1021 aaagcctccg cctcggcgtc ggtcggcgtc acgacgaagt agcgccggga cccgccgctc
     1081 atcctggccg cctcggcggt catgagcgcc gcgagcggtt gctccagctc cgagcagtcc
     1141 accgggaagg agccttcgtc gatgcgccga agggcctcgg ccaacggcgg atggcccgcg
     1201 agtcgtttcg atagcgtttc aaggcgtaag gagttcatat aaaacgtccg atatttgaag
     1261 gaggatagcg cgccgaccat agtacacgaa tcgcggcggc gcgcacaagg agacgcagca
     1321 gtgcatccga atcgaggcgt cttggccgcg ctggtcgccg ccctctgcct gggcgccgcg
     1381 gccgcccaag ccgaactaac cattagcttc ttcgacaagg cggtctatac gcctggctcg
     1441 gacgtctacg tcaaggtgac cgtcaggaac gactcgccgt caacctggcg cttcaagctg
     1501 gccgacgaga aacgcctgtc ggtcggcttc gaggtccgct ccctggccaa tcgccggctc
     1561 gaggcgagcg attcctggaa acgggccatg tcgtcgagcg ctccggtcta ctaccgcgag
     1621 ctcgccatcc agcccggcga ggaatactcc ttcgtcgaga atgtggccga ctacgtggcc
     1681 ctatcggaac cgggcgcctt cttcgtcgta tgcaccctct atccggagct ctcgggcagg
     1741 acggcggaat cgtcgacgat acgctcgaac gcgctgtccc tctcgatcag gcccggagcg
     1801 ccgacccccg ccgtggccga gcgcttcgtg gccggcaccg cggagatcct caaggccgag
     1861 cgcatcgggc ccgacgaggt cgtctccagg accatacggg ccaggcagaa gggccgatgg
     1921 aacgagttct tcctctatct tgacgtcgag cgcctcctca aggccaatag cgacaagagc
     1981 cggtcatacg accgcgagtc cgacgacggg cggcggcgca tgatcgagtc gtacaaggcc
     2041 gacttgatgg ccagcgtcgt cgacgccgac attgtcgtgg taccgagctc cttcgatata
     2101 ctcgagacgc ggtacggagc ctcgtacggt acggtgcgcg taacgcagaa gttcgactac
     2161 gacggcttca agatggtaaa ggaatatacc tacgagctcg agcgccgcga cgatatatgg
     2221 tatattgtgg cgtacaccgt acggaacaag ggtaccgaat gaaggctctc atcgtcgtcc
     2281 aggacgacgc cgtggcggag gtggtcagct tctacctgca ccccctcggc ctggacatca
     2341 tccattaccg ggatcccctc aaggccctgg acaacctcga cgagatcatg ccagacgccg
     2401 tgatcatgag cgcccgggac ttcccgcggc actggaaggc catcgtcgtc ggactacgct
     2461 cggcgaggcc caagtcggcc tgcgcggtca tcctgctcaa gggcgactac ttcccgttcg
     2521 aggaggcggc caaggccgcc tacctcggcg tgaacggcgt cgtcaaggag gacctctccg
     2581 acaaggccga gctcgaccgc ttccagcaat tgctcaagcg ctacgtcgag gtggacgacg
     2641 ccagagtctc cgaccgccac gcgcccggcc cctgggaccg gctcggcttc atcttctgtc
     2701 atcctgtaac ctaccagccg atagccggag ccatcgagac catttcaagc tccggcctgt
     2761 cgttcatacc cgagacgaac gccgcggtca gcgacatgga acccggcctc gtgctcgaag
     2821 aggcgtcact caggctcgat agggacatcc tatcgttacg ctgcaagctg atacgcaatg
     2881 ggccggtcat ggccttctcg ttcgagggat gcgagcctga gcgcgacgcc atcgccgact
     2941 acatagccgg aagggccgag cgcgagataa aagccctgtt gaaccattga agcactcggt
     3001 gctattatgt ggacatgaat gtcaacgtac cgtgcgatca gcaacgaatc gctgatttcg
     3061 ctagaaaact gaaggcagtc gggcatccca tacggctcag gctgctatgc ctcatcgccc
     3121 gagaacagga tccatgcgta tcggacctct ggcagtgtct cgaacagccc cagccggtca
     3181 tttcccagca cctggccata ctgaagcagg ccgggatcgt cagcgccgag gtccagaaga
     3241 ccaagcgcgt ctactcgatc agcgatccgt tcatcagcga catgatcagg aagatggtaa
     3301 ccgaggccta aaggccgacg cgatcagggc tggcctctcc tgaccgccga gcgcggatcg
     3361 cttagcctcg cgccggcccg cgccgatagc ggcgcgatcc cctgatcggc tatcaccccc
     3421 cacagcatca gggccggagc cctggcgccg gcgacgtaca gcgcgtcggc caaggtgaac
     3481 aagttcgccg cgaagccagt ctcgtccaag taggccatga cgtccctcct gcccagagcc
     3541 ctcgccatga agttctgcag gtccgtccac acatagtcgc cgtctctggc cttgagcatg
     3601 gctatcgccc tcgagaggat catgttgacg gctatggccg cgtgcctcgg ggcggcggcc
     3661 ctcccccgct ccaggtagaa cgccgccagg gaggcatgag ctgacttcga gtaggagtcg
     3721 tctaggcggt acagcgtcat gaagcgattg aagccctgct ccgtcagcgt cgccaacatc
     3781 gcctcctcga ggtaccggtc cacgcctgag tcgagcccct ctatgacgcg cagcatcacg
     3841 ttctcccagg cgacatactc cgatctcgcc tcgtacagtt cggccatggc gtagagtatc
     3901 gaataggcct cgccgggtac ctccagagcc tcgcgcatcc cgtaggcgcg ttcgtactgg
     3961 agctcggcga gggcgagctc cccctcgatg aagaacagcc ggcccttcca gtactcagct
     4021 tcaggataga gggcgaggag gcgcacgcac tctcgcaggc gctcgatgga gtcgccgagc
     4081 tcgcccaggg atcggtactc gatcacgagc aggagcacct cgacgag
//
LOCUS       DAOPHV010000191         4113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_29477_length_4113_cov_3.707491, whole genome shotgun sequence.
ACCESSION   DAOPHV010000191 DAOPHV010000000
VERSION     DAOPHV010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29477_length_4113_cov_3.707491"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLQ29_06245"
     CDS             complement(<1..196)
                     /locus_tag="PLQ29_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86284.1"
                     /translation="MLASPVDWDALERGVHGGKKKEIDARKPKGFPDHIPFIHAIPDP
                     HVERWVLNDSAAFKAIFGKGC"
     gene            complement(215..445)
                     /locus_tag="PLQ29_06250"
                     /pseudo
     CDS             complement(215..445)
                     /locus_tag="PLQ29_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024555.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            774..1223
                     /locus_tag="PLQ29_06255"
     CDS             774..1223
                     /locus_tag="PLQ29_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPG86285.1"
                     /translation="MRVISHTIHYGIGELRSMIVKKPRGGEYEPLLGPTGRALWEDDS
                     AELPGERFALDPDTGAEGSNYGRVRHGGSILRQSPDEGKASDWLHVSIPGHGDVNVWR
                     VIAGAWCERRDGATQVHHINNNGFDNRPSNLLWVSQAEHRAIEQGAF"
     gene            1777..3060
                     /gene="rsgA"
                     /locus_tag="PLQ29_06260"
     CDS             1777..3060
                     /gene="rsgA"
                     /locus_tag="PLQ29_06260"
                     /inference="COORDINATES: protein motif:HMM:NF015170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HPG86286.1"
                     /translation="MTKDYSPEGWGWRREHHASYETMAGLWPAARGMLPARVVGRDRG
                     AYRVVAPDFSGRPGFEAAAVSAAAGRAGAPGEYSGVALSGAYARAIEEADSGDRGRAS
                     GVLEYPACGDWVLVDLDGGYPRIQATLGRLSAVARGRAGRTSEAQVIAANIDTLIVVL
                     ALDGGRNFLPRLAERALVLARASGAAPVLALNKRDLADEETRLAAEAELSRLAPGVPV
                     LSTSARTGAGIAELAALFGPGETIALLGKSGMGKSALVNALAGAGSGEAAPGAGPSGA
                     GDVETAPPPEPGLRAIEGAVRDGDRRGRHTTTASRLYRLDSGLLVVDAPGFRELKVLG
                     DEEATADVFAEVSELAVSCRFSDCTHTNEPGCAVRAALDSGELEPGRFSDYLELEAER
                     ASYERRHSARLSASDERRWKLIAKARRRLKDAERG"
     gene            3127..>4113
                     /locus_tag="PLQ29_06265"
     CDS             3127..>4113
                     /locus_tag="PLQ29_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86287.1"
                     /translation="MALRSFRAAFVTAGLAAIILAAGPAWAQDALPPEGPPPEGAPPS
                     GEAPLEPEDLVLATLALDIASSDYYALAAWARSLGLDESGTAQELRARLYKHFGVSAP
                     PSAAASSKTITIESADRTEYLSARDDGESTVRFEGRVSISVKDDDRGETLTIRADTIV
                     VNRDANLLSARGDIVFERRKASGSDYFIGEALELDMDDWSGAFLDGESKRAAGEESGD
                     ALFFRADDIVKRGSDVLVFKDGVVSSCDDESPHYSIRASRIWILGGNEWAMLNATLSV
                     GELPLLYLPFFYYPGEEIAFNPVFGYEERFGRYVQTTTYLVGRKEVKEQEISL"
BASE COUNT          651 a         1308 c         1493 g          661 t
ORIGIN      
        1 cgcatccttt tccgaaaatc gctttgaacg ccgctgagtc attcagcacc catcgctcga
       61 cgtggggatc gggtatagcg tgtatgaacg ggatgtgatc ggggaaaccc ttaggtttcc
      121 ttgcgtcgat ttcctttttc ttgccgccat gaacgccacg ctcgagcgcg tcccagtcca
      181 ccggggaagc cagcacgtcg gatagtgcta cactgtgtac cgggggcttc tggttatgta
      241 ttgtttcttc ggcgttcggg ccccaatgat tggtcgtgga tatccatagc cggttggaga
      301 aggtcgtcgt gcgtagctgg tcgtccagga aggagcggct cgaggtggcc aggaagctat
      361 cgacggcggg cttgtcgatg atcgcgtcct cggcgtagca cttacactgg atggcccaat
      421 agtcgccgct cccggtacga gccacaaggt ctatgccggt atccttaccg ccgaaatctt
      481 aacgcccggg gaaatcgttc cagagccaga cgtccgtcaa ggcatcttta tagagcggat
      541 ccgtacgcaa ccaggcctgc atgaggcgct cgaaacgggc gcccttatcg cgttcggaga
      601 atgaatgcga tctatagtga ttgaggattg cctcgaaacc catgttttac cccgtcgtta
      661 cgaatacgag cattgtatag cctcgatcgg tcgagggcaa cagaacgtgt cgaccgtgcc
      721 tgccttgctc cccacggctc ctcccccatg ctatgctcta cgcaggagga accatgcgcg
      781 tcatcagcca tacgatccac tacggcatag gcgagctacg ctcgatgata gtcaagaagc
      841 cgaggggagg cgagtacgag cccctgctcg ggcccacggg acgggcgctg tgggaagacg
      901 acagcgccga attgccgggc gagcgcttcg cgctcgatcc ggataccggg gccgagggca
      961 gcaactacgg gcgcgtgcgc catggcggct cgatactcag gcaatccccg gacgagggca
     1021 aggcgagcga ttggctccac gtctccatcc ccgggcacgg ggacgtcaat gtctggcgcg
     1081 tgatagccgg cgcgtggtgc gagcggcgcg acggcgcgac ccaggtgcat cacatcaaca
     1141 acaacggctt cgacaaccgg ccttccaacc tgctctgggt atcgcaggcc gagcaccgcg
     1201 ccatcgagca gggcgcgttc taggcgcgtt tccccggggg gccgcgggat cgccgaggct
     1261 cgtacccggg cgccgtcgtt aacctgcgaa catggcgagt caggttcaac gacggcagcg
     1321 attgcggctt tagtccgcga tcgccgagga agcccgcgat cgccgaggaa gcccgcggcg
     1381 ggggccgcgt ccggctcagg cggccgggcc tacgcgcgtg gcggccccct tcgttcctga
     1441 ttaaaactat actttccctt gcgacgcctc gtcgtcgggg cgtacagtac ggataggttc
     1501 ggcgacagcc ggtccggccg ggcgcatagc gccggcatcc gagcgctcgt ccgtaaagga
     1561 ggcttattat gaagaaggcc actgctagga gcggagcggc gttcgggtag gccgcgcgaa
     1621 ccggaccgtg ggtccggctc gtctacagta caaaaaacgt agtcctaccc tccgaggcgg
     1681 tcatcgccgc gccctcccca tgccatcccg tcatcccgcc gcgcgtcagg ctcgcggggt
     1741 aatcgcgcat cgctggaact catcgaaagg tagactatga cgaaggacta tagccccgag
     1801 ggatggggct ggaggcggga gcatcacgct tcctatgaga ctatggccgg cctgtggccg
     1861 gccgcgaggg gcatgctgcc ggcgcgggtc gtcgggcggg accgcggggc ctaccgcgtc
     1921 gtggcgcctg acttctccgg gcggccgggc ttcgaggcgg ccgcggtctc ggcggcggct
     1981 ggcagggccg gcgctcccgg ggagtactcg ggcgtcgcgc tgagcggcgc gtacgctcgg
     2041 gccatcgagg aggccgactc gggcgaccgg ggccgggcct cgggcgtgct cgagtatccg
     2101 gcctgcggcg actgggtgct cgtcgacctg gacgggggct acccgcgcat ccaggcgacg
     2161 ctcgggcggc tgtcggcggt ggcccgcggc cgggcgggcc ggacgagcga ggcgcaggtg
     2221 atcgcggcga atatcgacac gctgatcgtc gtgctggcgc tcgacggcgg gcggaatttc
     2281 ctgccgaggc tcgcggagcg ggcgctggta ctcgcccggg cgtcgggcgc ggcgccggtg
     2341 ctggcgctca acaagcgcga cctggccgac gaggagacga ggctggccgc cgaggcggag
     2401 ctgtcgcgcc tggcgccggg ggtgccggtg ctgtcgacga gcgcgcggac cggggcgggg
     2461 atagcggagc tggcggcctt gttcgggcca ggcgagacta tcgccctgct cggtaagagc
     2521 ggcatgggca agtcggcgct ggtcaacgcg ctcgccggcg ccgggagcgg cgaggcggcg
     2581 cccggcgccg ggccgtccgg ggccggtgac gtcgagacgg cgccgcctcc ggagcccggg
     2641 cttcgggcta tcgagggcgc ggtgcgcgac ggcgaccggc ggggccggca cacgacgacc
     2701 gccagccggc tctaccggct ggactcgggg ctgttggtgg tcgacgcgcc gggcttccgc
     2761 gagctcaagg tgctcggcga cgaggaggcg acggccgacg tcttcgccga ggtgtccgag
     2821 ctggccgtct cctgccgctt ctccgactgc acgcacacga acgagccggg ctgcgcggtg
     2881 cgcgccgcgc tggattccgg cgagctcgag ccggggcgct tctccgacta cctggagctg
     2941 gaggcggagc gggcgtcgta cgagcgcagg cactcggcca ggctgagcgc gagcgacgag
     3001 cgccgctgga agctgatcgc caaggcgcgt aggcggctga aggatgcgga gcgcggctag
     3061 ggcaggggcg gcgcgtcgtc tacattcggt agcaaaataa taggcgccgt tgtatcatgc
     3121 gccggtatgg cactccgatc gttccgcgcc gcgttcgtga ccgcgggcct ggccgcaatc
     3181 atactcgccg cgggacccgc ctgggcccag gacgcgctac ctccggaggg gccgccgcct
     3241 gagggcgcgc cgccctcggg cgaggcgccg ttggagcctg aggatctggt gctcgcgacg
     3301 ctcgccctcg atattgcgag ctccgactac tacgcgctcg cggcctgggc ccggtcgctc
     3361 gggctggacg agtcgggcac ggcccaggag ctgcgggcca ggctctataa gcatttcggc
     3421 gtctcggcgc ccccgtccgc cgcggcgtcg tccaagacga tcacgatcga gtcggccgac
     3481 cgcaccgagt acctgagcgc cagggacgac ggggagtcga cggtgcgctt cgaggggcgg
     3541 gtgtcgatct cggtcaagga cgacgatcgg ggcgagacgc tgacgatacg ggccgatacg
     3601 atcgtggtca atcgcgacgc caacctgctg tcggcccgcg gcgacatcgt cttcgagcgc
     3661 cgcaaggcga gcggctccga ttattttatc ggcgaggcgc tcgagctgga catggacgac
     3721 tggagcgggg ccttcctcga cggggagagc aagcgggcgg ccggcgagga gtccggcgac
     3781 gcgctgttct tccgggccga cgacatcgtc aagcgcggca gcgacgtgct ggtgttcaag
     3841 gacggcgtgg tcagctcctg cgacgacgag agcccgcatt actcgatccg cgcctccagg
     3901 atctggatac tcggcggcaa cgagtgggcc atgctcaacg cgacgctgtc ggtcggcgaa
     3961 ttgccgctcc tgtacctgcc gttcttctac tacccgggcg aggagatagc gttcaacccg
     4021 gtcttcggct acgaggagcg cttcggccgc tacgtgcaga cgacgaccta cctggtcggc
     4081 cgcaaggagg tcaaggagca ggagatcagc ctg
//
LOCUS       DAOPHV010000192         4082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_29775_length_4082_cov_3.779240, whole genome shotgun sequence.
ACCESSION   DAOPHV010000192 DAOPHV010000000
VERSION     DAOPHV010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29775_length_4082_cov_3.779240"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PLQ29_06270"
     CDS             <1..972
                     /locus_tag="PLQ29_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPG86288.1"
                     /translation="GMVPRGEVALIVAGIGVTNGLLDGGLFGIAIMMTLLTTLVAPFG
                     LSATLRIPGAGTRDPEASGESKAVDFDFPSEDVALLVTDTMAHELQAEGYYVRTMDIE
                     EGIAQVRRDELAFSMRLDGARLSVQAPEKTLAIVRTSVFEAVATLNQNFARLKSDFDP
                     ASIQRNDSGEVGPLGRSEAKAVCPSCVALELKADTKEGAIRELLELMAGNGLIDDTER
                     AFADILERERSMSTGMQHGVALPHAKTDAVSAMAASIGIKRDGLEFDSLDGQPARIIV
                     LLTTPKKNPGAHIQFLAGVGAALKDERRRGQILEATSAEDVIRLMGV"
     gene            complement(983..1753)
                     /locus_tag="PLQ29_06275"
     CDS             complement(983..1753)
                     /locus_tag="PLQ29_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86289.1"
                     /translation="MARASSGHGDDERERRRTWDAAGELFVLAPAIVNVALNHSVCIQ
                     FGLPLHPTEYFEVDQSAEPLVRYSDEERDEAVSTLERSIALARAAYSIDPAFGAMAAA
                     YGSSLPHGIGTFVYTSRRDKYTWRAAEPAKVRALASSVLKAGRPSLAVGAAHGSIMAG
                     LFLAELLGCELWFLRFSMFKRNDTAPIVSAHDERKLRAHGDGSDILVFDEDSASGATL
                     SLLSARVKEMAPEARTGAVIRHQSAAFRPDHVGRVWWD"
     gene            2179..4059
                     /locus_tag="PLQ29_06280"
     CDS             2179..4059
                     /locus_tag="PLQ29_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG86290.1"
                     /translation="MAIMEIIGLSKDFGGTPILKNVSLTIEPGEKVALVGRNGCGKTT
                     LLRVAMGLDDDYKGVVRTAPGARLAYVRQKAPDFLPGESAAAYVCRDALERRAELERL
                     AETMAGANERAAAEALARYAALREAYDDDGGDAAEEAADRLLQKAGLGHRADAAAETL
                     SGGEKNVLALVRAVRRMPDLLVLDEPGNHLDMAGLAWLEDFIATLPCAVLLVSHDRRM
                     LDRVATSVLELETGGLTRYAGTYSAYRLARLKQAAGQGQDWQADRKRIERLEALVRRF
                     ADIARSRPDPAWGKRLRAKRSQLERVRAEATERPDLGDRDARVSFLGEASKADLALRI
                     DGYAKAYGDRVILEPSGFTVMNGERVALVGPNGSGKTSLVRDIVEFGSWDDERLRVGP
                     SMVVGYCAQAQEVFDPGRTVEEEFLKLLPTRKDALAHLGRFLFSWDDLDKRISSLSGG
                     ELNRLQLARASAMRANFLILDEPTNHLDIPTREAVEDALADFDGTVLVVSHDRYFIEK
                     VAERIVFIDRRRLVEYEGGFAEYWRDVGSAGLSAGRRSTPRGLEGRGRSTASSGASRG
                     SGESEGLEQRIEAMEREKERLERASATAFDERDFKRAKAVAAELERHNAVLKKLWAEY
                     VG"
BASE COUNT          697 a         1320 c         1411 g          654 t
ORIGIN      
        1 ggcatggttc cgcgcggcga ggtagcgctg atcgtagccg gcatcggcgt taccaacggc
       61 ctcctcgatg gcggactctt cggcatcgcc atcatgatga cgctgctgac gaccctggtc
      121 gcgccgttcg ggctatcggc gacgcttcgc atccccgggg cgggtacgag ggatccggag
      181 gcttccggcg agtcgaaggc tgtcgacttc gattttccgt ccgaggacgt cgccctgctc
      241 gtcaccgata cgatggctca cgagctgcag gccgagggct actacgtgag gaccatggat
      301 atcgaggagg gcatcgccca ggtcaggcgc gacgagctgg ccttttcgat gcggctggat
      361 ggggcgcgac tgagcgtcca ggcccccgag aagacgttgg ctatcgtcag gacctcggtc
      421 ttcgaggccg tagctaccct caaccagaat ttcgccaggc tcaaatcgga tttcgacccg
      481 gcgagcatcc agcggaacga ctcgggcgag gtcggccccc tcggccggtc ggaagccaag
      541 gcggtctgcc cgtcctgcgt cgcgctcgaa ctgaaggcgg ataccaagga aggggccata
      601 cgcgagctgc tcgagctgat ggccggcaac gggctgatcg acgatacgga gcgggccttc
      661 gccgacattc tcgagcgcga gcgctcgatg agcaccggta tgcagcatgg cgtcgccctg
      721 ccgcacgcca agaccgacgc ggtcagcgct atggcggctt ccataggcat taagcgggac
      781 gggcttgagt tcgactcgct cgatgggcaa ccggccagga tcatcgtgct tctgacgacg
      841 cctaagaaga accctggcgc ccatatccag ttcctcgcag gcgtcggggc cgcgctgaag
      901 gacgagcggc gcagggggca gatactcgag gccacgagcg ccgaggacgt catccggctg
      961 atgggcgtct aagggcgggc cgtcagtccc accatacccg gcccacgtgg tcggggcgga
     1021 acgcggcgct ctggtgccgt atgaccgccc cggtcctggc ttccggcgcc atctccttga
     1081 cgcgagccga tagcagcgac agggtcgcgc cggacgcgga gtcctcgtcg aacaccagta
     1141 tgtccgatcc gtccccgtgg gcgcggagct tccgctcgtc gtgggccgag acgatcggcg
     1201 ccgtgtcgtt gcgcttgaac atgctgaagc gcagaaacca taactcgcag ccgaggagct
     1261 cggccaggaa gagccccgcc atgatggatc cgtgggcggc cccgaccgcc aggctcggcc
     1321 ttccggcttt gagcaccgag ctggccagcg ccctgacctt cgccggttcc gcggccctcc
     1381 aggtgtactt atcgcgcctg gaggtgtaga cgaaggtccc tatcccatgc ggcaggctcg
     1441 agccgtaggc ggccgccatg gcgccgaagg ccggatcgat ggagtaggcg gccctggcca
     1501 gggctatgga gcgctcgagc gtcgatacgg cctcatcgcg ctcctcgtcg ctatagcgga
     1561 cgaggggctc cgccgattgg tcgacctcga agtactcggt cgggtgcagg ggcaggccga
     1621 actggatgca gaccgaatgg ttgagcgcca cgttgacgat ggccggggcc agtacgaaca
     1681 gctcgccggc ggcgtcccag gttcgccgcc gctcgcgctc gtcgtcgccg tggccggagg
     1741 aggctcgggc cacaggagcg ataccagctc ggccgatcgc ccaatcacct cggcgtaggg
     1801 acgctcaaaa tccatcgcgt ccggtgcttc gtcccaatcg catgacagga actcatctat
     1861 catgggcctt agtatacgct cttcccggcc aggtcgtcca aggccgcctc gccgctagct
     1921 tgcgtcgcgg cgtccaccat gctacagtgg cctcgtgaag acaacgaacc gttcgatcga
     1981 cctccaggac gttctggcag tccgctccgg aagcggctcc ctcgactgca ggagaggttc
     2041 gctcgaaacg gtaaatggag atatttgaga tatcaagtat cgtataccga gccgcgcggc
     2101 ccctatcggg atcgcgcggt tttttttgtg tcgatccgat tcggggtccc cggccgatgg
     2161 tgacgaaggg agcatcgcat ggcgatcatg gagatcatcg gactcagcaa ggacttcggc
     2221 ggaacgccca tcctcaagaa cgtatcgctg actatagagc cgggcgagaa ggtagcgctg
     2281 gtagggcgga acggctgcgg caagacgacc ctgctacgcg tggcgatggg actggacgac
     2341 gactacaagg gtgtcgtacg cacggcgcct ggcgcgcgcc tggcctatgt gcgacaaaag
     2401 gcgcccgatt tcttgcccgg cgagagcgcg gccgcctacg tctgccgcga cgcgctcgag
     2461 cggcgcgccg aactcgagcg gctcgccgag accatggcgg gcgccaacga gcgggccgcg
     2521 gccgaagcgc tggccaggta cgcggcccta cgcgaggcct acgacgatga cggcggagac
     2581 gcggccgaag aagcggcgga ccggctactc cagaaggccg gcctcgggca ccgggcggac
     2641 gccgcggccg agacgctgtc cggcggcgag aagaacgtgc tcgccctcgt ccgcgccgtc
     2701 aggcgcatgc cggacctgct cgtgctcgat gagccgggca accacctgga catggccggc
     2761 ctagcctggc tcgaggactt catcgccacg ttgccctgcg cggtgctgct agtctcccat
     2821 gaccggcgca tgctcgaccg ggtggccacc tcggtgctcg agctggaaac cggcgggctc
     2881 acgcgctacg ccggtaccta ttcggcctac cgtctcgcgc gcctcaagca ggcggcgggg
     2941 caggggcagg actggcaagc cgaccgcaag cgtatagaac ggctcgaagc cctcgttcgg
     3001 cgcttcgccg atatagcgcg ctcgaggccg gacccggcct ggggcaagcg cctgcgcgcc
     3061 aagcgtagcc agctcgaacg cgtcagggcg gaggctaccg agcgtcccga cctaggagac
     3121 cgcgacgccc gggtcagctt cctcggcgag gcctcgaagg ccgacctggc cctgcggatc
     3181 gacggatacg cgaaggccta cggggaccgc gtcatcctcg aaccgtcggg ctttaccgtg
     3241 atgaacggcg agcgggtcgc cctggttggc ccgaacggct cgggaaagac gagcctcgtg
     3301 cgcgacatcg tcgagttcgg atcctgggac gacgagcgcc tgcgcgtcgg gccgagcatg
     3361 gtggtcggct actgcgccca ggctcaggag gttttcgatc ccggacgcac cgtcgaggag
     3421 gaattcctca agctgctgcc gacgagaaag gatgcgctgg ctcacctcgg gcgcttcctt
     3481 ttctcatggg acgacctgga taagcgcatt tcgagcctgt ccggcggcga gctcaaccgc
     3541 ctccagctgg ctagggcctc ggcgatgcgg gcgaatttcc ttatattaga cgagcccaca
     3601 aaccacctcg atatcccgac gcgcgaggcg gtggaggatg ccctggctga ttttgacggc
     3661 acggtgcttg tcgtgtctca tgaccgttat ttcatcgaga aggtggccga gcgtatcgtg
     3721 ttcatcgacc ggaggcgcct ggtcgagtac gagggcggct tcgccgaata ctggcgcgac
     3781 gtgggctcgg ccggcttgag cgccgggcgg cgctccactc cccgcgggct cgagggccga
     3841 ggcagatcca ccgcctcgag cggcgcctcg cgcggttcgg gcgagagcga gggcctcgag
     3901 cagaggatag aggctatgga gcgcgagaag gaacggctcg aacgcgcgtc cgccacggcg
     3961 ttcgacgagc gcgacttcaa gagggccaag gccgtagccg ctgagctcga gcgccacaac
     4021 gccgtgctca agaagctgtg ggccgagtac gttgggtgat cgcctttggc gacggctcgg
     4081 tc
//
LOCUS       DAOPHV010000193         4053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_30062_length_4053_cov_3.833667, whole genome shotgun sequence.
ACCESSION   DAOPHV010000193 DAOPHV010000000
VERSION     DAOPHV010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30062_length_4053_cov_3.833667"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PLQ29_06285"
     CDS             complement(<1..846)
                     /locus_tag="PLQ29_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86291.1"
                     /translation="MKILCVLLPMQDSSGIGARDNVPLASARAMAWAEETEALSRDEW
                     AFLDGNAADHGGDAAVIASALGSRPELMLFELHAWNLERSLWIARRLRSLLPSTHFAA
                     LGPEVVQGMPVFKAQAFDALIEGEPEAAVVELLADLSARSIKPRYAGSPSVELERLPD
                     PYLSGALPIAPGKTVLIEASRGGATYPAFLRPYPGPRFFARDTAPRVLRLASDGGVDE
                     ARFIDRRLDARPDFKAFVKSLAAVNETGVALSGRLDPAGLDDEAAALLADAAFVSVSA
                     WLGSVN"
     gene            complement(880..1650)
                     /gene="aat"
                     /locus_tag="PLQ29_06290"
     CDS             complement(880..1650)
                     /gene="aat"
                     /locus_tag="PLQ29_06290"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HPG86292.1"
                     /translation="MRKPRFPWLGEDDGFAFPPVSGASPDGVLCVGGNLSPGMLLSAY
                     RQGIFPWFNDDEPIVWWSPDPRFVLEPAELHVSGTMRKLIKKRRFRLSLDRAFGEVIR
                     ACSASPRRGQRGTWITSDMIDAYERMHALGYAHSVEAWLDGALVGGLYGLSLGSAFFG
                     ESMFSREPDASKAAFIPFVWALAEAGCTLVDSQVYTDHVASLGGKSVRRVEYLAMLER
                     ALASPTIKGDWAAAFPSFPSSRRWLELCEGGVSEVSST"
     gene            complement(1662..4040)
                     /gene="clpA"
                     /locus_tag="PLQ29_06295"
     CDS             complement(1662..4040)
                     /gene="clpA"
                     /locus_tag="PLQ29_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HPG86293.1"
                     /translation="MKVNQQVQAIFNAAYNEAKLRGHEYLTPEHILYASLSFEQVRSI
                     LEACDADIEHIKVGMEAYFEQKIPAVKDQEPIQTAGFQSVIERAVTQSQGAGKTEVGI
                     PEILVSLFDEERNYSAYYLRKAGVNRLELLKVISHGSDDEYGPSEDGDIDSAAGPFSS
                     PDDDDDIDGAEARQKPRSTVKAGALEKFATDLTKAAREGRLEPVIGREQEIERTVQVL
                     CRRLKNNPIHVGDAGVGKTAITEGLAQRIVEGRVPPKLRDYTIYSLDMGSIIAGTKFR
                     GDFEERIKRVVDELLKKDRAILFLDEIHTIGGAGAVSGGSMDASNLLKPALTSGKLRC
                     IGSTTYEEYNKFFDKDRALSRRFQKIDIVEPTVGEAIEILKGLRPKYETYHDVTYSDE
                     ALELAARLSAQFITERRLPDKAIDVLDEAGAWARIRAYKAASHEGASHEGATATAAGD
                     AAGNEVNGAGATETLAIGRGEIETVVAKIARIPEKSVSQTERDRLATLEADLKASVFG
                     QDAAIETVAKAVKRGRAGFRAPDKPVANFLFVGPTGVGKTELARTLADTLGVALHRFD
                     MSEYQEKHTVSRLIGSPPGYVGYEEGGLLTDAIRKTPHAVVLLDEIEKAHPDVYNILL
                     SIMDYATLTDNQGRKADFRNVVLIMTSNAGARDIGKPMIGFGERAFTEAAIDEAVQKA
                     FTPEFRNRLDAVVRFGKLPREVIERIVVKALDDFRAQLADKKVILEATDACVKLLAEL
                     GYSDEFGARNVGRVVEERIKSWFVDEVLFGALKDGGAARADVEDGRIVIKAL"
BASE COUNT          641 a         1296 c         1390 g          726 t
ORIGIN      
        1 gttgacgctg ccgagccagg ccgagaccga aacgaaggcg gcgtcggcga ggagggccgc
       61 ggcctcgtcg tcgagcccgg ccgggtcgag cctgcccgat agggcgacgc cggtttcgtt
      121 gacggcggcc agcgacttga cgaaggcttt gaagtcgggc cgagcgtcca gcctccggtc
      181 gatgaagcgg gcctcgtcga cgccgccatc ggaggccagc ctgagcacgc ggggagccgt
      241 gtcgcgagcg aagaacctgg gccccggata ggggcgtagg aaggccggat acgtggctcc
      301 cccgcgggag gcctcgatca gcacggtctt gccgggagcg ataggcaggg cgccgctcag
      361 gtaggggtcg ggcaggcgct cgagctctac ggacggcgag ccggcatagc gcggcttgat
      421 cgacctggcg gacagatcgg cgagtagctc gacgaccgcc gcctcgggct cgccctcgat
      481 caacgcgtcg aaggcctgcg ccttgaagac gggcatgccc tggaccacct cggggccgag
      541 cgccgcgaaa tgcgtcgaag gcaggagcga tcggagccgc ctggctatcc acagcgaacg
      601 ttcgaggttc caggcgtgga gctcgaacaa catgagttcg gggcgagagc ctagggcgga
      661 cgcgatgacc gccgcgtcgc cgccgtggtc cgcggcgtta ccgtcgagga acgcccattc
      721 gtcgcgcgat agcgcttcgg tttcctcggc ccaggccatg gctctggcgg aggccagcgg
      781 cacgttgtcg cgggcgccga tgcccgagct gtcctgcatc ggcaatagta cgcataggat
      841 cttcatctcg tggatattcg ctccgttccg tcgtgacggt caggtagagg atacctcgga
      901 aacgccgccc tcgcatagct cgagccaccg ccgcgacgac gggaaggacg gaaaggcggc
      961 ggcccagtcg cccttgatcg tcggggaggc aagggccctc tcgagcatgg ccaggtattc
     1021 gacccgccgc actgacttgc cgccgagcga cgcgacgtgg tccgtgtaga cctggctgtc
     1081 gacgagggtg caccccgcct cggcgagcgc ccagacgaag ggtatgaagg cagccttgga
     1141 cgcgtcgggc tcccggctga acatcgactc gccgaagaag gccgagccga gcgacaggcc
     1201 gtagaggccg ccgacgaggg cgccgtccag ccaggcctcg accgagtgcg cgtagcccag
     1261 ggcgtgcatg cgctcgtagg cgtcgatcat gtccgaggta atccaggtgc cgcgctggcc
     1321 gcgcctgggc gaggctgagc aggcgcgtat cacctcgccg aaggcccggt cgaggcttaa
     1381 acggaagcgg cgcttcttga tcagtttgcg catggtgccg gagacgtgga gttcggccgg
     1441 ctcgagcacg aagcgcgggt cggggctcca ccagacgatg ggctcgtcgt cgttgaacca
     1501 cgggaagatg ccctggcggt aggcggatag cagcatgccc ggcgacaggt tgcccccgac
     1561 gcagagcacg ccgtccggcg acgcgccgga tactggcggg aaggcgaagc cgtcatcctc
     1621 gccgagccat ggaaagcgcg gctttctcat ctcgggggcc gctagagcgc cttgatgacg
     1681 atccggccgt cctctacgtc ggcacgcgcg gcgccgccgt ccttgagagc gccgaagagc
     1741 acctcgtcga cgaaccagga cttgatgcgt tcctcgacga cgcggccgac gttgcgggcg
     1801 ccgaattcgt ccgaataccc gagttccgcc aggagcttga cgcaggcatc ggtcgcctcg
     1861 aggatcacct tcttgtcggc gagctgggcg cggaagtcgt cgagcgcctt gaccacgata
     1921 cgctcgatca cctcgcgcgg aagcttgccg aagcgcacga ccgcgtcgag ccggttgcgg
     1981 aactccgggg taaaggcctt ctgtacggcc tcgtcgatcg cggcctcggt gaacgcccgc
     2041 tcgccgaagc ctatcatcgg tttgccgatg tcccgagcgc cggcgttgct cgtcatgatc
     2101 agcacgacgt tgcggaaatc cgccttgcga ccctggttgt cggtcagcgt cgcgtagtcc
     2161 atgatggaca gaaggatatt gtagacgtcg gggtgggcct tctcgatctc gtcgagcagg
     2221 acgaccgcgt gcggcgtctt gcgtatcgcg tcggtcagga ggccgccctc ctcgtagccg
     2281 acgtagcccg gcggcgagcc tatgagccgg ctcacggtgt gcttctcctg gtattcgctc
     2341 atgtcgaagc ggtggagggc gacgccgagg gtatcggcca gagtgcgcgc cagttcggtc
     2401 ttgcccacgc cggtcgggcc gacgaacagg aagttggcca caggcttatc cggcgcgcgg
     2461 aaaccggccc ggccacgctt gacggccttg gctacggttt cgatggccgc atcctgcccg
     2521 aagacgctcg ccttgagatc cgcctcgagg gtggcgagcc tatcacgctc ggtctgcgac
     2581 acgctcttct cggggatacg cgctatcttg gcgacgaccg tctcgatctc gccgcggccg
     2641 atggccaggg tctcggtggc gcccgccccg ttcacctcgt tcccggccgc gtcgccggcg
     2701 gcggtcgcgg tggctccctc gtgcgaggct ccctcgtgcg aggcggcctt gtacgcgcgt
     2761 atcctcgccc aggcaccggc ctcgtcgagc acatcgatgg ctttgtcagg cagtcgacgc
     2821 tcggtgatga actgggccga caatctcgcc gctagctcga gcgcctcgtc ggagtaggtc
     2881 acgtcgtggt aggtctcgta cttgggccgc agccccttga gtatctcgat cgcctcgccg
     2941 acggtcggct cgacgatgtc tatcttctgg aagcgcctgg acagcgcccg atccttgtcg
     3001 aagaacttgt tgtattcctc gtaggtcgtc gagcctatgc agcgtagctt accggaggtc
     3061 agggccggct tgaggaggtt ggacgcgtcc atggagccgc ccgagacggc gccggcgccg
     3121 cctatcgtgt ggatctcgtc gaggaacagg atggccctgt ccttcttgag tagctcgtcg
     3181 accacgcgct tgatacgctc ctcgaagtcg cctctgaatt tggtgccggc gatgatcgag
     3241 cccatatcga gcgagtagat agtatagtcg cgcagcttag gcggcacccg cccctcgacg
     3301 atgcgttggg cgaggccctc ggtgatggcg gtcttaccga ccccggcgtc gccgacgtga
     3361 atgggattgt tcttgagccg cctgcaaagg acctggacgg tgcgctctat ctcctgttct
     3421 cggccgatga ccggctccag gcgaccctcg cgggcggcct tggtcaggtc ggtggcgaat
     3481 ttctcgaggg cgccggcctt gaccgtcgag cgcggcttct gtcgggcctc ggcgccatcg
     3541 atgtcgtcat cgtcgtccgg tgacgaaaag ggcccggcgg ccgaatcgat atccccgtct
     3601 tcgctcggac catactcgtc gtccgacccg tgcgatatga ccttgagcag ttccagacgg
     3661 ttgacgccgg ccttgcgtag gtagtaggcg gagtagttcc gctcctcatc gaacagggaa
     3721 accaggattt ctgggatgcc gacctcggtt ttgccggcgc cctgggactg cgtcaccgct
     3781 cgttcgatga cgctctggaa cccggccgtc tggatgggct cctggtcctt gaccgcggga
     3841 atcttctgct cgaagtaggc ctccatgccg actttaatat gctcgatgtc ggcgtcgcag
     3901 gcctccagga tggagcggac ctgctcgaac gacagcgagg cgtacaggat atgctcaggc
     3961 gtcaggtact cgtgtcccct gagcttggct tcgttgtacg cggcgttaaa tattgcttga
     4021 acctgctggt taaccttcat acctgccctt aag
//
LOCUS       DAOPHV010000194         4021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_30416_length_4021_cov_4.957136, whole genome shotgun sequence.
ACCESSION   DAOPHV010000194 DAOPHV010000000
VERSION     DAOPHV010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30416_length_4021_cov_4.957136"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="PLQ29_06300"
     CDS             <1..1185
                     /locus_tag="PLQ29_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HPG86294.1"
                     /translation="ERYREAYPDAGAFLESRGVPSFGASEPALYMDGGAWFERAMELI
                     AGAEDYILISSFLITDIDRQAAIFDALAEQMTDGVRVYMIVDSASYYRTYPMDAEPIP
                     ASTPLARNLGIPLIEYNPIRGRRIFTLLGLLDRDHRKFWVVDGRTVVTGGQNVDYDSL
                     RDVGNGGCVDAMIEYESPGAAAFLRDSFVRTWNAYSLDPLDPDDFAVGDADTPYRLSV
                     IDQGLRSPGLVTDMFDGFMAFASEELWLVQCYAYLTPSLLDRVRFAVDRGVRVNVVLS
                     REHVTARALYGSYYGMLDLLDAGASVYLYESDVGSLLHFKLIMADGELASVGSANYNL
                     RSHTTSRELSFIVSDQESLAMIRAELDALLASCRPVGREEARRYRGLPYLLQNLLMQF
                     WG"
     gene            1189..1743
                     /locus_tag="PLQ29_06305"
     CDS             1189..1743
                     /locus_tag="PLQ29_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86295.1"
                     /translation="MKLVRTFACAALLAAAALPTSAEPRSPWLGGVLFSTRGFSEDSS
                     HAAGEYLDLGLFVDPFDFAVLRPSASLRLIVPLFPFDAGRIEGAAGLDLELFGLRKHP
                     LRAIMAQASAYAPALSAARSFSFVDPLSGEWVVGAHPIRLRTGDARYSFLSPELRFGP
                     GFEPAGWSLKLFEFSYLIFAEARR"
     gene            1767..2282
                     /locus_tag="PLQ29_06310"
     CDS             1767..2282
                     /locus_tag="PLQ29_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86296.1"
                     /translation="MAVLLTAALASASAQEWGVAVGSWSFAGGGPGPGAYACAGLSAG
                     LGQRLELEAFGVARATPAPFGDALAGAILGFSVFGPRRTTYFNMVLDLGFLQTVVEDG
                     IPGLGPSYAMLRLSPLVVGTPYYGHRDRIFSLGLVYDLGSGALSATWNVLIVHWYPGS
                     HAWLPAGDRER"
     gene            2287..>4021
                     /locus_tag="PLQ29_06315"
     CDS             2287..>4021
                     /locus_tag="PLQ29_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPG86297.1"
                     /translation="MLTPTSASGRKAILGAVLVALFALPDRVSADDLMLASTPVFVGR
                     EAFEARVAARVAARSGGREPVGLVLSGGSARAFAHIGVLRRLEEDGVVPDYIVANSMG
                     SIIGLLYAAGLSPDQIYGLVSGTELGALFEPTLPVSGGVLDPRRFSDLIRLYLGDLRL
                     EDLPIPIVVICEDLRTKREIRLAEGDLVTVLEAAYALPVFFPPVELDDYLLIDGGVTN
                     LVPLGAAREFSDTVIVSSTFYDNPRLNLRNPIIVLNTSIDIGKRRAGVADLLEYPDAL
                     WIRCSVESFSFMSFDRLAELDAEGYRSADALSDRLAGIASGGVDEALAAVRLERGAAI
                     AEAARAWAPFERAPAARPALSVTPTVRSSAYPGDPYLYLDSVFVGAGLGLRWGALEAS
                     LDAGGDWRAYGDGSFVPAASVSASVDPLPWLRLETLVLADWESLSEHGDDGERVGGVS
                     IPALYQRSALRAALVRGGSRLELSSAVEADGLASEGAAWLLTSGARYVASRPASSKAA
                     AGGALRELSASLGHQLAGDRDEHLAWADASAALSPFASSAALRPLTARLGAMARVALS
                     AGAAAPFYLSDA"
BASE COUNT          505 a         1432 c         1357 g          727 t
ORIGIN      
        1 gagcgctacc gggaggccta tccggacgcg ggcgccttct tggagtcgcg cggcgtgcct
       61 agcttcgggg cctccgagcc ggctctgtac atggacggcg gcgcttggtt cgagcgcgcg
      121 atggagctga tagccggcgc cgaggactac atactcatat cgtcgttcct cataaccgat
      181 atcgaccggc aggccgccat cttcgacgcg ctggccgagc agatgactga cggcgtccgc
      241 gtgtatatga tcgtcgactc ggcgtcgtac taccgcacct atccgatgga cgccgagccc
      301 atcccggcct cgacgccgtt ggccaggaac ctcggtatac cgctgatcga gtacaacccc
      361 atacgcggcc ggcgcatctt cacattgctc ggcctgctcg accgcgatca ccgcaagttc
      421 tgggtcgtcg atggacggac cgtggtcacc ggcggccaga acgtcgacta tgactcgctg
      481 cgcgatgtag ggaacggggg ctgcgtcgac gcaatgatcg agtacgagtc gccgggagcg
      541 gccgccttcc tgcgcgacag cttcgtgcgt acctggaacg cgtattccct cgacccgctc
      601 gatcccgacg acttcgccgt cggcgacgcc gacacgccgt atcggttgtc ggtcatcgac
      661 cagggcctgc gtagcccggg gctcgtcacc gacatgttcg acggcttcat ggctttcgcg
      721 tccgaggagc tgtggctcgt gcagtgctac gcctacctga cgccgtccct gctcgacagg
      781 gtgcgcttcg cggtcgaccg cggcgtgcgc gtcaacgtgg tgctgtcgcg cgagcacgtg
      841 acggcgcggg cgctgtacgg gtcgtactac ggcatgctcg accttctgga cgcgggcgcc
      901 tcggtctacc tctacgagtc tgacgtcggc tccctgctcc attttaagct gatcatggcg
      961 gacggggagc tggcttcggt cggttcggcg aactacaacc tgcgttcgca tacgacctcg
     1021 cgggagctgt ccttcatcgt ctccgaccag gagtcgctgg ccatgatacg cgccgagctc
     1081 gacgccctgc tcgcctcctg ccggccggtc ggccgcgagg aggccagacg ctaccggggc
     1141 ctgccgtatc tcctgcaaaa cctgcttatg cagttctggg gttagactat gaagctggta
     1201 cgtacgttcg cctgcgccgc cttgctcgcg gcggcggccc tgccaacctc ggccgaaccg
     1261 cgctcgccct ggctcggcgg cgtcctattc tcgaccaggg gcttttccga ggactcgtcc
     1321 cacgcggccg gagagtatct agacctgggc ctcttcgtcg atccgttcga cttcgccgtc
     1381 ctgaggccgt cagcctctct ccggctgatc gtccccctgt tcccgttcga cgcggggcgg
     1441 atcgagggcg ccgctggcct ggacctggag ctgttcggcc tgaggaagca tccgctccgc
     1501 gcgatcatgg cccaggccag cgcctacgcg ccggcgctgt cggccgccag gagcttctcc
     1561 ttcgtcgatc ctctgagcgg ggagtgggtc gtcggcgctc atcctatcag gctcaggacc
     1621 ggtgacgcgc gctatagctt cctgtcgccg gagctgcgct tcggcccggg gttcgagccg
     1681 gccggctgga gcctcaagct gttcgagttc tcatacctga tctttgcgga ggcgcggcga
     1741 tgaggcggcc atctatcatc gcgttcgtgg ctgttctcct gacggcggcg ctggcaagcg
     1801 cgtccgcgca ggaatggggc gtcgctgtcg gctcgtggtc gttcgccggc ggcggtccgg
     1861 ggcccggcgc gtacgcctgc gccggccttt cggccgggct cggccagcgt ctggaactcg
     1921 aggccttcgg cgtcgcgcgg gccacgccgg cgcctttcgg cgacgcgctg gccggcgcca
     1981 tcctcggctt ctccgtgttc gggcccaggc ggaccaccta cttcaacatg gttctcgacc
     2041 tgggtttcct gcagaccgtc gtcgaggatg gtattcccgg tctgggcccg tcgtacgcga
     2101 tgctcaggct gtcgccgctg gtcgtcggca cgccgtacta cggccaccgc gaccgcatct
     2161 tctcgcttgg gctcgtgtac gacctcggct ccggcgccct gtcggccacc tggaatgtcc
     2221 tcatcgtcca ctggtacccc ggtagccacg cgtggctacc cgccggagac cgtgaaaggt
     2281 aacgccatgc tcactcctac aagcgcctcg gggcgcaaag ccatactggg ggccgtcctg
     2341 gtcgcgctct tcgcgctacc ggaccgcgtc tcggccgacg acttgatgct cgcgtcgacg
     2401 cccgtcttcg tcggcaggga ggccttcgag gctcgcgtcg ccgcgcgcgt agccgctcgt
     2461 tccgggggcc gcgagccggt cggactcgtg ctgtcaggcg gctcggccag ggccttcgcc
     2521 catatcggcg tgctcaggcg gctcgaggaa gacggcgtcg tgccggacta catcgtcgcc
     2581 aactccatgg gctccatcat cggcctcctc tacgcggccg gcctctcgcc cgaccagatc
     2641 tacggcctgg tatcgggcac cgagctcggc gccctgttcg agccgaccct gccggtctcc
     2701 ggcggcgtgc tcgacccgag gcgcttcagc gatctgatcc ggctctacct cggcgacctg
     2761 aggctcgaag atctacccat ccccatcgtc gtcatctgcg aagacctgcg gaccaagcgc
     2821 gagatacgcc tggccgaggg cgacctggtc acggtcctcg aggcggccta cgcgctgccg
     2881 gtgttctttc cgccggtcga gctcgatgac tacctgctga tcgacggcgg cgtgacgaac
     2941 ctggtgccgc tcggcgcagc ccgggaattc tccgataccg tcatcgtctc gagcaccttc
     3001 tacgacaacc cgcggctaaa cctgcgcaac cccatcatcg tgctcaatac ctcgatcgat
     3061 atcggcaagc ggcgcgccgg cgtggccgac ctgctcgagt acccggacgc gctctggata
     3121 cgctgttcgg tcgagtcgtt ctcgttcatg tcgttcgacc ggctggccga gctcgacgcc
     3181 gagggctacc gatcggccga cgctctgtcg gaccggctgg ccgggatcgc gtcgggcgga
     3241 gtcgacgagg ccctggcggc ggtacgcctc gagcggggcg cggccatcgc cgaggcggcc
     3301 cgggcctggg ctccgttcga gcgggcgccg gcggcgaggc cggccctgtc ggtcacgccg
     3361 acggtgcgct cgagcgccta tccgggcgac ccgtacctgt acctggactc cgtattcgtc
     3421 ggcgccgggc tgggcctgcg ctggggcgcg ctcgaggcgt cgctcgacgc gggcggcgac
     3481 tggcgagcgt acggcgacgg ctccttcgtc ccggccgcgt ccgtctcggc gagcgtcgac
     3541 ccgctgccgt ggctcaggct cgagaccctc gtcctggccg attgggaaag cctctccgag
     3601 cacggcgacg acggcgaacg ggtaggcggc gtctcgatac cggcgctgta ccagcggagc
     3661 gcgctccggg ccgcgctcgt ccggggcggc tctcgcctcg agctgtcctc ggcggtcgag
     3721 gccgacgggc tggcgtccga aggagcggcc tggctcctga ccagcggcgc caggtacgtc
     3781 gcgtcgcgtc ccgcgtccag taaggcggcc gcgggcggcg cgctccggga gctgtccgcg
     3841 agcctcggcc atcagttggc tggcgaccgg gacgagcacc tggcctgggc cgacgcctcc
     3901 gcggccctat cgccgttcgc gtcgtcggca gcgctccgcc cgctgaccgc gaggctcggc
     3961 gccatggcgc gggtcgcgct gagcgccggc gccgccgcgc ccttctacct ttcggacgcc
     4021 g
//
LOCUS       DAOPHV010000195         4013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_30510_length_4013_cov_3.745579, whole genome shotgun sequence.
ACCESSION   DAOPHV010000195 DAOPHV010000000
VERSION     DAOPHV010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30510_length_4013_cov_3.745579"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PLQ29_06320"
     CDS             complement(<1..723)
                     /locus_tag="PLQ29_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HPG86298.1"
                     /translation="MRNAFFLGKGGVGKTTSSAAFALAEARAGKRVLIASLDPAHNLG
                     DVLGKRLKDKPLRVEENLDALEIRLSAWVDRYLKDSRDEMKETYSYAGALNLDSFFDI
                     MKYSPGTEEYAVLWAIEHIHCELSPDYDLVVYDTPPTALSLRFLAMPAISNMWITELA
                     KLRERILQRRQIVTKINPESPVASSCVDKDDDKVYGRLGSIKRRLALLQKLFSEESFV
                     AVVINPDSLSVSEALRIKEELER"
     gene            complement(750..1028)
                     /locus_tag="PLQ29_06325"
     CDS             complement(750..1028)
                     /locus_tag="PLQ29_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86299.1"
                     /translation="MGKLADKARAAYGIVRQINRERATQMIEFELKELENIFTLLVMG
                     GFVGMPSPPAPIAMELLPLLEREMNIMMSRSDFAQDPLGAMMGLLEID"
     gene            complement(1158..2840)
                     /locus_tag="PLQ29_06330"
     CDS             complement(1158..2840)
                     /locus_tag="PLQ29_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HPG86300.1"
                     /translation="MTTMIALFALALYLVFYFTFGKTLRDKLLKSDKAPAAPSERLSD
                     GVDYVPTNKFVLFGHHFASIAGAGPITGPAMAVAWGWLPGLLWIWFGNIFLGAIHDYL
                     ALAASVRYDGRSVQFVAQDMIGKKAGKTFSWFILFLCILVVAAFGDIVAGQFAADGRV
                     FFSFVFFCVAAVIAGYFMYKSKLGLPGGTIIGIALVVVAFIAGNALPVKWAKDIYFLV
                     ILIYIILASTLPVNLLLQPRDYLSSYFLYFGLLAGGIAALISFRPLDAIPLFTSFNAK
                     VISGVPSPIWPTIPLVIACGALSGFHSLVASGTSSKQLRTENDALFVGYGAMLVEGFL
                     STLVVISIAGFGFFALGDKLMATPALGRFVQSFAKMVSMNLPFLSFDFMTLFAAVWVS
                     TFALTTLDTTNRLGRYIIQEMALPLKDKKPGVYKVFGNKWVASMIIAFIGLFLARSGG
                     YSVLWPAFSGANQLLASVVMLTVTVWVKKKLDPKYTMTVFIPGIILWVTVTAALVWYE
                     FAVIPTFFRDMAKVQNVITGVIVGLITLFMLILNFIMIGGFAKNWKTKAQAA"
     gene            3038..>4013
                     /locus_tag="PLQ29_06335"
     CDS             3038..>4013
                     /locus_tag="PLQ29_06335"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HPG86301.1"
                     /translation="MRMTFRWFGTGFDSIPLRYIAQIPGLYGVIATLYDKQAGERWER
                     SRIKALKDDCAEYGLALAGIESVNVHDAIKAGLPERDEYIDAYIATLEALGEEGVDLV
                     CYNFMPVFDWTRSDLAKRRPDGSTVLAYDQAVIDSLAPDLMAESVDRGSGGFTLPGWE
                     PERMARVRELFGLYRDVDAERLFANLVYFLDAIMPTCRRYGIRMAIHPDDPAWPVFGL
                     PRVVTGEESLLRLMRAVDDPHNGVTLCTGSLGTNPDNDIVGTIHALEGRVHFAHVRNL
                     RHEAPGKFEEAAHLSADGSFDMYAFMKALYDIGFSGPVRPDHGRTIWDE"
BASE COUNT          794 a         1238 c         1290 g          691 t
ORIGIN      
        1 gcgctcgagc tcttccttga tgcgcagcgc ttccgatacc gacagtgagt ccgggttaat
       61 gacgaccgcg acgaagctct cctcgctgaa cagcttctgg agcagcgcca ggcgacgctt
      121 gatcgagccg agccggccgt agaccttgtc gtcgtccttg tcgacgcagc tcgaggcgac
      181 cggcgactcg ggattgatct tggtaacgat ctgccgacgc tgcaggatac gctcgcgtag
      241 cttggccagc tcggtgatcc acatattcga tatggccggc atggccagga accgcaggga
      301 aagagccgtc ggaggcgtgt cgtagacgac gaggtcgtag tccggcgata gctcgcaatg
      361 gatgtgctcg atggcccaga gcaccgcgta ctcctcggtg cccggggagt atttcatgat
      421 atcgaagaac gagtcgaggt tcagggcgcc cgcgtaggag taggtctcct tcatctcatc
      481 acggctatcc ttgaggtagc gatccaccca agcggacagc cggatctcga gcgcgtcgag
      541 gttctcttcg accctgagcg gcttgtcctt gagcctcttc cccagcacgt cgcccaggtt
      601 atgcgccggg tcgagcgacg cgatcagcac ccgtttgccc gcccgcgcct cggccagcgc
      661 gaaggccgcg gaactcgtcg tcttgccgac gccgcctttt cctaagaaga acgcgtttct
      721 catccagatt attccttgat ttcagagact cagtcgatct cgagcaaccc catcatggcg
      781 ccaagcggat cctgggcgaa gtccgagcgc gacatcatga tattcatctc gcgctccagt
      841 agcggcagca gctccatcgc tatcggcgcc ggaggcgaag gcatgccgac gaagccgccc
      901 atgacgagta gcgtgaagat gttctcgagc tccttgagtt cgaattcgat catctgggtg
      961 gcacgttcgc ggttgatctg ccggacgatg ccgtaggcag ccctggcctt gtctgcgagc
     1021 ttccccattc ccgactccga ttggtgatac tagacaaaat cggagggcct ccgtaggggg
     1081 ccccccgacg atcgagcctg tcgtcgcccc cgcccggcgc gagccaggca ggtagcatcg
     1141 acgtgaatcc ggaggaatta agccgcctgg gccttggtct tccaattctt ggcgaaaccg
     1201 ccgatcatga tgaagttcag gatcagcatg aacagcgtga tgagcccgac tatcacgccg
     1261 gtgatgacgt tctgcacctt ggccatgtca cggaagaagg tcggaatgac ggcgaactcg
     1321 taccagacga gggccgcggt caccgtgacc cacagaatga tgccaggaat gaatacggtc
     1381 atcgtgtact tgggatcgag cttcttcttg acccagaccg tcaccgtgag catgacgacc
     1441 gaggccagca gctggttggc gccggagaag gccggccaca gcacggagta gccgccggag
     1501 cgcgcgagga acaggccgat gaaggcgatg atcatcgacg cgacccactt gttgccgaac
     1561 accttgtaga cgcccggttt cttgtccttg agcggcaacg ccatctcctg gatgatgtag
     1621 cggccgaggc ggttggtcgt gtcgagggtg gtcagcgcga aggtcgacac ccataccgcg
     1681 gcgaatagcg tcatgaagtc gaaggacagg aacggcaggt tcatgctgac catcttggcg
     1741 aaggactgca cgaagcggcc gagcgccggg gtggccatga gcttgtcgcc gagcgcgaag
     1801 aaaccgaagc cggcgatcga gatgacgacg agcgtcgaga ggaagccctc gacgagcatg
     1861 gcgccgtagc cgacgaacag ggcgtcgttc tcggtccgca gctgcttgga gctggtgccg
     1921 gaggcgacga gggagtggaa gcccgatagg gcgccgcagg ctatgaccag cgggatcgtc
     1981 ggccagatcg gcgacggtac gccggagatc accttggcgt tgaagctcgt gaacaacggg
     2041 atggcgtcga gcggcctgaa gctgatcagg gccgcgatgc cgccggcgag caggccgaag
     2101 tacaggaagt acgacgacag gtagtcgcgc ggctgcagga gcaggttcac gggcagggtc
     2161 gaggccagga tgatgtagat gaggatgacc aggaagtaaa tatccttggc ccacttgacc
     2221 ggcagggcgt tgccggcgat gaacgcgacc acgaccaggg ctatgccgat gatcgtgccg
     2281 ccgggcaggc cgagcttgct cttgtacatg aagtaaccag cgatgaccgc cgccacgcag
     2341 aagaagacga aggagaagaa cactcgaccg tcggcggcga actggccggc gacgatgtcg
     2401 ccgaaggccg cgacgacgag gatgcacagg aacaggatga accagctgaa ggtcttgccg
     2461 gccttcttgc ctatcatgtc ctgggcgacg aactgtaccg agcggccgtc gtagcggacg
     2521 gacgccgcga gcgccaggta gtcgtggatg gcaccgagga agatattgcc gaaccagatc
     2581 cacaggaggc cgggtagcca gccccaggcg acggccatgg ccggtccagt gatcgggccg
     2641 gctccggcga tcgaggcgaa gtgatggccg aacaacacga acttgttggt cggcacgtag
     2701 tccactccgt ccgacaggcg ctcggacggg gccgccggag ccttatcgct cttgagcagc
     2761 ttgtcgcgca gcgtctttcc gaaggtgaag tagaacacga ggtagagtgc cagtgcgaaa
     2821 agcgctatca ttgtcgtcat cgcgctttcc tccttggggt taggttattt gaatacgtgg
     2881 acatcttaaa tctccgcata gaaacctgtc aatcatttta attatgaaaa tttgcgctcc
     2941 acccgcataa gaatcgtacc accggggacc gcttgcgccg ggcggcttct ttcggcacta
     3001 tggggctgga cggaccgatg gccgtcggga gaccagcatg cggatgacct tcagatggtt
     3061 cggtaccggc ttcgacagca tcccgctccg gtacatagcc cagatcccgg gcctgtacgg
     3121 cgtcatcgcg accctgtacg acaaacaggc cggcgaacgc tgggagcgct ctcggatcaa
     3181 ggcgctcaag gacgattgcg ccgagtacgg acttgccctg gcaggcatcg agagcgtcaa
     3241 cgtccacgac gccataaagg ccgggctacc ggaacgggac gagtacatcg acgcctatat
     3301 cgcgacgctc gaggccctcg gcgaggaggg cgtcgacctc gtctgctata acttcatgcc
     3361 cgtcttcgac tggacgcgca gcgacctggc aaagcgccgg cctgacggct cgaccgtgct
     3421 cgcctacgac caggccgtca tcgactccct ggcccccgac ctgatggccg agtccgtcga
     3481 ccgcggctcg gggggcttca ccctgcccgg ctgggaaccc gagcgcatgg cccgcgtccg
     3541 cgagctgttc gggctgtacc gggacgtcga cgcggagcgc ctgttcgcca acctggtgta
     3601 cttcctggac gccatcatgc cgacctgccg ccgctacggc atacgcatgg ccatccaccc
     3661 ggacgacccc gcctggccgg tcttcgggct gccgcgcgtg gtcaccggcg aggagagcct
     3721 cctccgcctg atgcgggcgg tcgacgaccc gcacaacggc gtgacgctgt gcaccggctc
     3781 gctcggcacc aacccggaca acgatatagt cggaacgata cacgcgctcg agggccgcgt
     3841 ccacttcgcc cacgtgcgca acctgcgcca cgaggcgccc ggcaaattcg aggaagccgc
     3901 ccacctctcg gcggacggtt ccttcgacat gtacgccttc atgaaagcgc tctacgatat
     3961 cggcttctcg ggccccgtgc ggcccgacca cggccggacc atctgggacg agc
//
LOCUS       DAOPHV010000196         4011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_30533_length_4011_cov_4.194135, whole genome shotgun sequence.
ACCESSION   DAOPHV010000196 DAOPHV010000000
VERSION     DAOPHV010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30533_length_4011_cov_4.194135"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PLQ29_06340"
     CDS             complement(<1..599)
                     /locus_tag="PLQ29_06340"
                     /inference="COORDINATES: protein motif:HMM:NF016166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF432 domain-containing protein"
                     /protein_id="HPG86302.1"
                     /translation="METWKRWDLPKNEGTRVHRIGDRRICVAVDMVGDQYVLAAMALP
                     PEASRGSRLALPSLSDKAWTRFFMDKAEDYELAPAYPPMPTCIRLKQPFSLAPGSTVE
                     GWIFSRIEASILVNGARIASFPLATPYKTLYGSPEAGVICRYDEEEFLAAAEPLASSL
                     HADQRFVAHPVRLRNASAEPVEVSDLCIYGEQLSIVGDE"
     gene            743..1687
                     /locus_tag="PLQ29_06345"
     CDS             743..1687
                     /locus_tag="PLQ29_06345"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPG86303.1"
                     /translation="MATIRGRVEAVGPLRFDRYASEELKMLTRSQLKARFVSLSVNGK
                     DAKLSRSVEAGDDFALELLDEEDRSSASAPEDIPLSVLYEDARVVVVDKPQGMVVHPA
                     HGNWSGTLANALLGRFAAEGRAAPARAGIVHRLDKDTSGVLIVGKSAAAQEALAAQFR
                     ARSTVKTYLAIVRRGPSADSGRLDGWLARDPRERKRFAPSSEGVGKRALTEWTVLARA
                     GGYAVLALGLRTGRTHQLRVHCKALGCPILGDPIYGSPDGRFPGASLMLHAWELRIRL
                     PGDDEPSTFRAPVPERFAATAAALGLDGFPLPPGGAAL"
     gene            complement(1684..1956)
                     /locus_tag="PLQ29_06350"
     CDS             complement(1684..1956)
                     /locus_tag="PLQ29_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPG86304.1"
                     /translation="MSKKIYVGNMNYATTEDSLRELFGQFGTVESVAVVVDRATGRAK
                     GFGFVEMESPEAAQAAIQKLDGQEFEGRKLRVNEAQEKPRDSGPRY"
     gene            2186..3712
                     /gene="rny"
                     /locus_tag="PLQ29_06355"
     CDS             2186..3712
                     /gene="rny"
                     /locus_tag="PLQ29_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015713068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPG86305.1"
                     /translation="MAILYIALPLAGLFLGWMIRWLYARFQLTAAEQKAARITQEAVK
                     QAEAQKREILLEAKDQLIRERNQQERETRERRAEIQRYERRLLQKEENLDKKVESLEK
                     QETALDERDKVMAEKEEFLSGQEERYRKELERISGLSAEEAKRLIIQGLENEARHDAQ
                     ALLNKIEQEAQTAAEKRTRDILVTTMQRLAADVTAEVTVTSVSLPGDEMKGRIIGREG
                     RNIRTLETLTGVDVIIDDTPEAVVISCFDPVRREIARVSLERLISDGRIHPARIEEVV
                     QKVSREISQKIFEEGEKVVFDLGLHNLNPELIRALGRLHFRTSYGQNVLSHSKEVAVI
                     AGMLAAELGANRDLAKRGALLHDIGKGVESDSDQNHAEIGMDLARKLGEDPRVVNAIG
                     SHHADIEPICLESVLCQIADAISAARPGARRETLDNYVKRLENLEGIAEGFSGVDKAY
                     AIQAGRELRIIVNTEKVSEDDAKDLCKSIAKKIETDMRYPGRIKVTMIRETRIVEYAR
                     "
     gene            3810..>4011
                     /locus_tag="PLQ29_06360"
     CDS             3810..>4011
                     /locus_tag="PLQ29_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HPG86306.1"
                     /translation="MMAENSTRVLMIGDVIGDAGVAAAAGRLRALADETGAALVVVNG
                     ENAAGGFGLTRETADKLFAAGAD"
BASE COUNT          734 a         1310 c         1365 g          602 t
ORIGIN      
        1 ttctcgtcgc cgacgatgga cagctgctcg ccgtagatgc agaggtccga gacctccacg
       61 ggctcggccg acgcgttgcg tagcctgacc gggtgggcga cgaagcgctg gtcggcgtgc
      121 agcgacgagg cgagcggctc ggcggcggcc aggaattcct cctcgtcgta gcggcagatg
      181 acacccgcct cgggtgagcc atacagggtc ttgtacggcg tggctaacgg gaaggaggct
      241 atcctcgcgc cgttgaccag gatgctggcc tcgatgcgcg agaagatcca gccctcgacc
      301 gtggagccgg gcgccagcga gaacggctgc ttgagcctga tgcaggtggg catgggcggg
      361 taagccggcg ccagctcgta gtcctcggcc ttgtccatga agaagcgcgt ccaggccttg
      421 tcggacagcg aaggtaacgc cagccgggat cccctggacg cctcgggagg cagggccatc
      481 gcggctagta cgtactggtc gccgaccatg tcgaccgcca cacagatgcg gcggtcgccg
      541 atgcggtgga ctcgcgtgcc ctcgttctta ggcagatccc aacgtttcca cgtttccata
      601 ggctcctcgc gggctcggat tgcgtgcccg gcccggaatt cgtatactgt cgccgcgccg
      661 cgcgcgctcc cgattccgta ccgggcccgc gtcctagtat acgggcccgc ggcgccgggg
      721 taaagcgcgg cggaaagagg ctatggctac catacgaggc agggtcgagg cggtcgggcc
      781 actcaggttc gatcgctacg cgtccgaaga actcaagatg ctcacccgga gccagctcaa
      841 ggcgcgcttc gtctcgctat cggtcaatgg taaggacgcc aagttgtcca ggtcggtgga
      901 ggccggcgac gacttcgcgc tcgagctgct cgacgaggag gaccgatcgt cggcttcggc
      961 ccccgaggat ataccgctgt cggtgctgta cgaggacgcg cgagtcgtcg tggtcgacaa
     1021 gccgcagggc atggtcgtcc acccggccca cggcaactgg tccgggaccc tggccaacgc
     1081 gttgctcgga cgcttcgccg ccgagggccg ggccgcgccg gcgcgagccg gcatcgtaca
     1141 ccggctcgac aaggatacct ccggcgtgct catcgtcggc aagagcgcgg cggcccagga
     1201 ggcgctcgcc gcccagttcc gcgccaggag caccgtcaag acttacctgg cgatagtcag
     1261 gcgcggcccg tcggcagatt ccggccggct cgacggctgg ctggcccgcg acccgcgcga
     1321 gcgcaagcgc ttcgcgccgt cgtccgaggg cgtcggcaag cgcgcgctta cggaatggac
     1381 ggtcctcgcc agggcggggg gctacgccgt cttggcgctc ggcctgcgga ccggcaggac
     1441 gcaccagctg cgcgtccatt gcaaggcgct cggctgcccg atactcggcg acccgatcta
     1501 cggctcgcca gacggccgct ttcctggagc ctcgctgatg ctacacgcct gggagctgag
     1561 gatcaggctg ccaggggacg acgagccgtc cacgttcagg gcgcccgtac cggagcgctt
     1621 cgccgcgacc gcggcggcgc tcggcctcga tggctttccg ttgccgccgg gcggcgcggc
     1681 gctctagtag cggggtcccg agtcccgggg cttctcctgc gcctcgttga cgcgcagctt
     1741 gcggccctcg aactcctgcc cgtccagctt ctggatcgcg gcctgggcgg cctcggggct
     1801 ctccatctcg acgaagccga agcccttggc gcggccggtg gccctgtcga cgacgacggc
     1861 taccgactcg accgtcccga actgcccgaa cagctcccgt aggctgtctt ccgtcgtagc
     1921 gtagttcatg tttccgacat agatcttctt ggacatcgcg cgaatctcca tgagccggcg
     1981 actgagccgg gttaggctgg cccgagacag ctcgggcggg cgccccgcgg cgccaatgcg
     2041 atgatacagc gggcccggcc cggcgtcaac gtggatagaa acgcgccatg gaagcggtta
     2101 ggcgtcggcg cgcgtatgtt ttcttgacaa aggctcgccg ttcactataa tgatagcgat
     2161 attctattaa aagctggagg atacaatggc tatactgtat atagcccttc ctcttgccgg
     2221 tcttttcctg ggatggatga tccgctggct gtacgccaga tttcaactta cggccgccga
     2281 gcagaaggcg gcgcgtataa cgcaagaagc ggtaaaacag gccgaggcgc agaagagaga
     2341 aatactgctc gaggccaaag atcagctcat cagggagaga aaccagcaag agcgggagac
     2401 tcgggaacgc agggccgaga tacagcgcta cgaacggcgc cttctacaga aagaagagaa
     2461 cctcgacaag aaagtcgagt ccctcgagaa gcaggagacc gctctcgacg aacgcgataa
     2521 ggtcatggcg gagaaggagg aattcctttc cggccaggag gagcgctacc gcaaggaact
     2581 cgaacgcatc tcgggcctga gcgccgagga agccaagcgc ctgatcatcc aggggctcga
     2641 gaacgaggcc aggcacgacg cccaggccct gctcaacaag atcgagcagg aagcccagac
     2701 ggcggccgag aagcgcacgc gcgacatcct cgtcaccacc atgcagcgcc tcgccgccga
     2761 cgtcaccgcc gaggtgaccg tcacgagcgt gtccctgccg ggcgacgaga tgaagggccg
     2821 catcatcggc cgcgagggcc gcaacatacg caccctcgag acgctgaccg gcgtcgacgt
     2881 gatcatcgac gacacgccgg aggccgtggt catctcgtgc ttcgacccgg tgcgcaggga
     2941 gatagcccgc gtatcgctcg agcggctcat ctccgacggg cgcattcacc cggcccgtat
     3001 cgaggaggtc gtccagaagg tcagccgcga gatatcgcag aagatcttcg aggagggcga
     3061 gaaggtcgtc ttcgacctgg gcttgcacaa cctgaacccg gagctgatcc gggcgctcgg
     3121 ccgtctgcac ttccgcacga gctacggcca gaacgtgctg tcgcactcga aggaagtggc
     3181 cgtgatagcg ggcatgctcg ccgcggagct gggcgccaac cgcgacctgg ccaagcgcgg
     3241 ggccctgctc cacgacatcg gcaagggcgt cgagtccgac tccgaccaga accacgccga
     3301 gataggcatg gacctggccc gcaagctcgg cgaggatccg cgcgtggtca acgcgatcgg
     3361 ctcgcaccac gccgacatcg agccgatctg cctcgagagc gtgttgtgcc agatagcgga
     3421 tgccatctcc gccgccaggc ctggcgccag gcgcgagacg ctcgacaact acgtcaaacg
     3481 gctcgagaac ctcgagggca tcgccgaggg cttctccggt gtcgacaagg cctacgccat
     3541 ccaggcggga cgggagctgc gcatcatcgt caacaccgag aaggtgtccg aggacgatgc
     3601 gaaggacctc tgcaagtcca ttgccaagaa gatcgagacc gacatgcgct atccgggcag
     3661 gatcaaggtg acgatgatcc gcgagacccg catcgtcgag tacgcgcgct agcagcggcg
     3721 acgaaggaac gaacgcaggg ccgcccgctt acgggcggcc cttttactat ggacggcgcg
     3781 ccccgagccc ggggccgccc ggaaggtcga tgatggctga gaactccacc agggtgctga
     3841 tgataggcga cgtgatcggc gacgccggcg tggccgccgc cgccggcagg ctgcgcgcgc
     3901 tggccgacga gaccggggcg gccctcgtcg tggtcaacgg cgagaacgcg gccggcggct
     3961 tcgggctgac cagggagaca gccgataagc tgttcgccgc cggcgccgac g
//
LOCUS       DAOPHV010000197         3995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_30707_length_3995_cov_3.185279, whole genome shotgun sequence.
ACCESSION   DAOPHV010000197 DAOPHV010000000
VERSION     DAOPHV010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30707_length_3995_cov_3.185279"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLQ29_06365"
     CDS             complement(<1..206)
                     /locus_tag="PLQ29_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPG86307.1"
                     /translation="MAKRKFFDSLSMDIGIDLGTCNTLVYVRGKGIVINEPSVVAVER
                     GTKRVIAVGSDAKKMLWKTPGNIIA"
     gene            complement(235..1200)
                     /locus_tag="PLQ29_06370"
     CDS             complement(235..1200)
                     /locus_tag="PLQ29_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG86308.1"
                     /translation="MNDISFGTARTTDPYLRKRRRRRLVIGLAVMLGVTATAVGVSLC
                     VSAIATARSSPKVSQQDVLALWASKDYLAVAQACEASLSVAPLDPFYLLFDGFASFYL
                     GLAETDGELRGVKMDEAIFSIRKALISPKLPLRPEAAYVLGKAYYHKGVDYYNEAIDF
                     LNESTSFGYYQADTWEYLALAARGAGRLAESLQYFDKAVELKPDSPELSLAAAAAFVE
                     AGDAAKGERLAAAALESTSDDFLAERGRFLLADIYRGNGRPDDALAQYEAIKALNGES
                     ADAWFYEGLVYSEAGDPIKARAAWRKAVSIDPMHTAARQKLSERS"
     gene            complement(1253..1652)
                     /gene="rnpB"
                     /locus_tag="PLQ29_06375"
     ncRNA           complement(1253..1652)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLQ29_06375"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1662..2468)
                     /locus_tag="PLQ29_06380"
     CDS             complement(1662..2468)
                     /locus_tag="PLQ29_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPG86309.1"
                     /translation="MERVAIEKDVRELPKSIAAQEEVLTRARKSYGDRSLRLEETQSR
                     VGQLRAELADVVAHKDKSEQQMDSITNPREFEALNKEITEAGLKEDQLRKDLKREEEN
                     LREAEVEVRASETLISSQEAEIAEKRERITNEAASMSAKVEKLKRDEAKTTAEIDPEV
                     VFKFERIIKSKQGVGIVPVKGVVCSGCSMILPAQFVNEVRQSDKIVFCPYCSRVLFYQ
                     EGDESQEDMIVDIESGSLADLDDFGDESDEEYDDESDDESDKDSMPDSDD"
     gene            complement(2609..>3995)
                     /gene="rpoD"
                     /locus_tag="PLQ29_06385"
     CDS             complement(2609..>3995)
                     /gene="rpoD"
                     /locus_tag="PLQ29_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HPG86310.1"
                     /translation="ELSKQMEEGENIIKGIIKKSGMLIPEFFQISVKAKREAGSQQRK
                     ENSERIAERRRLNQFYRDVLRDTQVDLKSYMESKRRIASRGGDVKSDENLIQVRDNLL
                     DRLGEADLHPEEINSFSEKFINAAKRIKKYRREQDRLERTLQVDNLRELRVLGRNLTL
                     KERREQIESDLGISSDEIKERIRQIQVTEKKLEELEYSFENDCDVIITMAKEINRGRN
                     MMKNAKDRLIKANLRLVVSIAKKYTNRGLHFFDLVQEGNIGLIKAVEKFEYRKGYKFS
                     TYATWWIRQAITRSISDQARTIRVPVHMIEQINKVVRESRQLMQKLGREPSDEEVAQQ
                     LGWPVSRVKSVKNVAREPISLETPIGEDEDSLLGDFIEDKEIENPSVQTDYKLLQEQI
                     RSVLSTLPPREQEVLRMRFGLDDGYSLTLEEVGLYFNVTRERIRQIEAKALKKLRHFK
                     RSQKLRDYVDH"
BASE COUNT          647 a         1253 c         1182 g          913 t
ORIGIN      
        1 gctatgatgt ttccaggggt cttccacagc atcttcttgg cgtcggagcc taccgctatg
       61 acgcgcttcg tgccgcgctc cacggcgacg accgacggct cgttgatgac tatgcccttg
      121 cctcgcacat aaacgagcgt attgcaggtg ccgagatcga ttcctatatc catcgacagc
      181 gagtcaaaga acttgcgttt cgccattata tgcgctcctt actgcggtta tccatcacga
      241 acgttctgat agtttctgcc gagccgcggt gtgcatcggg tctatggaca cggccttgcg
      301 ccacgcggcc ctcgccttga tcgggtcgcc ggcctcgcta tagacgagtc cctcgtagaa
      361 ccaggcgtcg gccgactcgc cgttcagggc cttgatcgcc tcgtactgcg ctagcgcgtc
      421 gtccgggcgg ccgtttccgc ggtagatgtc ggccaacagg aagcggccac gctcggccag
      481 gaaatcgtcg ctcgtcgatt cgagggcggc tgcggcgagc cgttcgccct tcgcggcgtc
      541 gccggcttcg acgaaggccg ccgccgcggc cagggacagc tcgggcgagt ctggcttgag
      601 ctcgacggcc ttatcgaagt actggaggct ctcggccagg cgtccggcgc cgcgcgcggc
      661 cagggccagg tactcccagg tatcggcctg atagtaaccg aaagacgtcg attcgttcag
      721 gaagtctatg gcctcgttat agtaatcgac gcccttgtga tagtaggcct tgccgagcac
      781 atacgccgct tcggggcgca aaggtagctt gggcgaaatg agcgccttgc ggatcgagaa
      841 aatagcctcg tccatcttga ccccgcgtag ctcaccgtcg gtctcggcca ggcccaggta
      901 gaaggacgcg aatccatcga aaagcagata gaacggatcg aggggggcga cagatagcga
      961 cgcctcgcag gcctgggcga ccgctagata atccttggag gcccagaggg cgaggacgtc
     1021 ctgttggcta accttaggcg acgagcgcgc cgtcgcgatg gccgatacgc acaaggatac
     1081 gcctacggcg gtggccgtta cgccgagcat gactgccaag cctatgacca acctgcgacg
     1141 cctgcgcttc cgtagatatg ggtcggtagt tctggcggtg ccgaaggata tatcgttcat
     1201 actactcggt tcgttgtatc acgccagcgc cgctatagtc aattaaagca aaaaaaggga
     1261 cgaacctata agccgggttc tgtaccgctt tcgcggcggc cgccatccat ctgcgccggg
     1321 gatcgctccc ctggcttccg cgacctaccc acggagtgtc gcctcgacct cgcggtcgag
     1381 gcgcggcgcg agccgcgcct ccgttgttcg gccttgctcg cgacgaggct tgccgtgccg
     1441 ttcgcgtcgc cgcgaacgcg gtgggctctt accccacctt ttcaccctta cccgcgcctt
     1501 tcggcgccgg cggttcgctt tctgttgcgc tatctgtcgc gcggccgtca ccggccgcgc
     1561 tcccggtcgt taaccggcgt cgcgccctat cgagcccgga ctttcctccg cgccagcccg
     1621 ggggctaacg cggcggcggc ccggtccgtc cctatactac cctagtcgtc cgagtccggc
     1681 atcgaatcct tgtcggactc gtcgtcggac tcgtcatcgt actcctcgtc ggactcatcg
     1741 ccgaaatcgt ccagatcggc cagagagccg gattctatat cgacgatcat gtcttcctgc
     1801 gactcgtcgc cttcctgata gaacagcacg cgagaacagt acgggcagaa tacgatctta
     1861 tcgctctgcc tgacctcgtt gacgaactgg gccggcagta tcatcgagca tccggagcat
     1921 acgacgccct tgacgggtac gataccgacg ccctgcttcg acttgatgat gcgctcgaat
     1981 ttgaacacga cctccgggtc tatttcagcg gtggtcttgg cctcgtcacg cttgagcttc
     2041 tcgaccttgg cggacatcga agcggcctcg ttcgtgatac gctcacgctt ctcggcaatc
     2101 tcggcctcct gggatgagat cagggtctcg ctggcgcgta cctcgacctc cgcctcgcgg
     2161 aggttctctt cctcgcgctt cagatcctta cgcagctggt cttccttgag accggcctcg
     2221 gtgatctcct tgttcagggc ctcgaattcg cgcgggttcg tgatggagtc catctgctgc
     2281 tcggacttgt ccttgtgggc tacaacgtcc gccagctcgg cgcgaagctg gccgaccctg
     2341 gactgcgtct cctcgaggcg caagctcctg tcaccgtagc tcttccgggc tctcgtgagc
     2401 acctcttcct gggccgctat ggacttgggg agttcgcgta cgtccttttc gatggcgacg
     2461 cgctccatga ggagctcctg aaacgttcgt agcctctcga aaagctcttc catcagcata
     2521 tgttttcacc tcgtcctgga taatttcgca agtaacgcgg gattatagca cgcggggcgc
     2581 gcggagatca acgacgcgcc cgcgacgact agtggtcgac gtagtctcgt agtttctggc
     2641 tacgcttgaa gtgccggagc ttcttgagcg ccttggcctc gatctggcgt atgcgctcgc
     2701 gcgtcacgtt gaagtacaga ccgacttcct cgagggtcag ggagtatccg tcgtcgaggc
     2761 cgaagcgcat acgcagtacc tcctgctcgc gcgggggcaa cgtcgacagg accgagcgta
     2821 tctgctcctg aagcagcttg tagtcggtct gcacgctcgg gttctctatc tccttgtcct
     2881 cgatgaagtc accgagcagg ctatcttcgt cctcgcctat cggcgtctcg aggcttatgg
     2941 gctcccgggc cacgttcttg acgctcttga cgcgcgaaac cggccagcct agctgctggg
     3001 caacttcctc gtcgctcggc tcgcggccga gcttctgcat cagctggcgg gactcgcgca
     3061 cgactttgtt gatctgctcg atcatatgca ccggcacgcg tatcgtcctg gcctggtcgc
     3121 tgatcgaccg ggttatggcc tggcgtatcc accaggtcgc gtaggtggag aacttgtagc
     3181 ccttgcggta ttcgaatttc tccaccgcct tgatgagccc gatgttgcct tcctggacga
     3241 ggtcgaagaa gtggagaccg cggttcgtgt acttcttggc gatggacacg accagccgca
     3301 ggttggcctt gatcaaccta tccttggcgt tcttcatcat gttgcggccg cggttgatct
     3361 ccttggccat cgtgatgatg acgtcgcagt cgttctcgaa cgagtactcg agctcctcga
     3421 gcttcttctc ggtgacctgg atctgcctga tgcgctcctt gatctcgtcg gagctgatgc
     3481 cgaggtccga ctcgatctgt tccctgcgct ccttgagagt caggttcctg ccgagcacgc
     3541 gtagctcgcg caggttatcg acttggagcg tacgttcgag cctatcctgc tcccggcggt
     3601 acttcttgat gcgcttggca gcgttgatga acttctccga gaaggagttg atttcctcgg
     3661 gatgcaggtc ggcctcgccg aggcgatcca gcaggttgtc gcgtacctgt atgaggttct
     3721 cgtcggattt cacgtcaccg ccacgggagg cgatgcgccg cttcgactcc atatacgact
     3781 tgaggtcgac ctgtgtgtcg cggagtacat ctcggtagaa ctggttgagc ctgcgtcgtt
     3841 cggcgatgcg ctccgaattc tctttgcgtt gctgcgaacc ggcttcgcgc ttggccttga
     3901 cggatatctg gaagaactcg ggtatcagca tgccgctctt cttgatgatc cccttgatga
     3961 tgttctcgcc ttcctccatc tgcttggata gctcg
//
LOCUS       DAOPHV010000198         3994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_30720_length_3994_cov_3.983244, whole genome shotgun sequence.
ACCESSION   DAOPHV010000198 DAOPHV010000000
VERSION     DAOPHV010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30720_length_3994_cov_3.983244"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1547)
                     /gene="argH"
                     /locus_tag="PLQ29_06390"
     CDS             complement(135..1547)
                     /gene="argH"
                     /locus_tag="PLQ29_06390"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPG86311.1"
                     /translation="MLKLWEMSPTGLDPRVERFLSSLSVDTRLFEDDLQGSAAHAAML
                     GRVGVVPEATASALVRELGAMLDEARAGALTPAEPAEDVHSFIEQELTRRLGSAGKTV
                     HAGRSRNDQVALDVRLYLRRAFAEAGNGVLDAIGAVCDLASANVETYMPSYTHLRRAQ
                     PSTLAFHLLAWCAALERDYGRFADAARRADECPLGSAAVAGSGLPLDRRAAAAALGFA
                     RPSRNAMDATGDRDACVEYAAAASTLLMHLSRFCEEIILWSSAEFGFMDIADDYSTGS
                     SIMPQKRNPDVAELIRGKAGRAFGSLVALLTIQKGLPLGYDRDLQEDKAALFDLRDTL
                     AGVLPAFVALVEALRPRPVAMAAALETGFAEATDLAELLVAKGVPFREGYAATKAAVA
                     AAAAEGVGLAGLSDADLAAASGAFAEAGIRASDLTAYLRPEACVGRRDGVGGPAPDRT
                     REEIARLRAFIALNSACAPA"
     gene            complement(1558..2877)
                     /locus_tag="PLQ29_06395"
     CDS             complement(1558..2877)
                     /locus_tag="PLQ29_06395"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPG86312.1"
                     /translation="MKKIALAYSGGLDTTVIVPWLKEHYDCDVVAVCGDVGQACDWDG
                     LEQKARSSGASKLVVNDLKKAFVEETLWPLVKAGALYEGRYLLGTAAARPLIAKALAE
                     TAIVEGCSAVAHGCTGKGNDQIRFEMGLKAFAPDMEIIAPWRIWDLGSREEEIAYLEA
                     HGIPTPFKKGDSYSRDDNLWHVSHEGLDLEDPMNEPVLEGMLKLCVPPDKAPDEAEYV
                     ELTFEKGIPVALNGERTDGVSLVARLNELGGRHGIGILDMVENRVVGMKSRGVYETPA
                     GAILMEAHQKLEQLCLDRKTMSFKATVAQRFAELMYEGEWYSPLMDAVNAFVDSTQEA
                     VGGVVKLRLWKGSLSCAGMSSPYSLYDHSLASFATGPLFDQRDATGFINLYSLPVKAR
                     SLMKARLEAESGKGAVPAGAVPAGAVPAGANGSGGSGLKAAKVAAAY"
     gene            3399..>3994
                     /locus_tag="PLQ29_06400"
     CDS             3399..>3994
                     /locus_tag="PLQ29_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86313.1"
                     /translation="MPRTITAQSSATTIDAPPEAIQYRDPDYQGLEAALDRRAAAGAN
                     PSELGALIAKEYSRRNRAIKDPGIHRLDPSSVADDYLYPRKDLNRFAVENWIRRVFLG
                     GLKPRIVGSSLVFGLGRLFSTYNDLGAQHATDVDLNIIVDDALPARLYAELGGRLGDL
                     RVELHQRFGILLDLHPDYTLLRESEVLGALSADDETRR"
BASE COUNT          681 a         1355 c         1282 g          676 t
ORIGIN      
        1 gaaggtcctg acggtctcga gcgcccgttc tatcgccgcc tcatccaacc cgcgcgccct
       61 acaccgatcc tggaattcgg cttgcatcag catcgcgtcc ccctcggcta ggcgatagct
      121 gaagtatagg cgatctaggc cggcgcgcaa gccgaattca gggctatgaa ggcgcgcaat
      181 cgggcgatct cctcgcgcgt acggtcgggc gccggtccgc cgacgccgtc ccgacggccg
      241 acgcaggcct cgggccgcag gtacgcggtc aggtccgagg ccctgatccc ggcttcggcg
      301 aaggcgccgg acgctgccgc gaggtcggcg tctgagagcc ccgctaaacc gactccttcg
      361 gcggccgccg cggcgaccgc tgccttggtc gccgcgtagc cttccctgaa cggtacgccc
      421 ttcgcgacga ggagctcggc gaggtcggta gcctcggcga agccggtctc gagggcggcg
      481 gccatcgcca cgggcctggg tctcagagcc tcgacgaggg cgacgaacgc cggcagaacg
      541 ccggccaggg tatcgcgtag gtcgaatagc gcggccttgt cctcctggag gtcgcggtcg
      601 tagccgagag gcaggccttt ctgaatcgtg agcagcgcga cgagggagcc gaaggccctc
      661 ccggccttgc ctcgtatcaa ctcggcgacg tccgggttgc gcttctgcgg catgatgctc
      721 gaccccgtcg agtaatcgtc ggctatgtcc atgaagccga actcggcgct cgaccacagg
      781 atgatctcct cgcagaagcg cgacaggtgc atgagcaggg tcgacgccgc ggccgcgtac
      841 tccacgcagg cgtctcggtc tccggttgcg tccatcgcgt tgcggctcgg tcgcgcgaag
      901 cccagagcgg cggccgccgc cctgcggtcg agcggcagtc ccgagccggc tacggccgcg
      961 gagccgagcg ggcactcgtc ggcgcggcgc gcggcgtcgg cgaagcggcc gtaatcgcgt
     1021 tcgagcgcgg cgcaccacgc gagcaggtgg aacgcgagcg tggacggctg ggcgcgccta
     1081 aggtgcgtgt aggacggcat gtacgtctcg acgttggccg acgcgaggtc gcagacggcg
     1141 ccgatcgcgt cgagtacgcc gttaccggcc tcggcgaagg cgcgacgcag gtacagcctc
     1201 acatcgagcg ctacctggtc gttgcggcta cggccggcgt gcacggtctt gccagcgctc
     1261 cccagcctac gcgtcagttc ctgctcgatg aagctatgta cgtcctcggc cggctcagcc
     1321 ggcgtcaagg ctcccgctcg agcctcgtcg agcatcgcgc cgagctcacg caccagcgcg
     1381 gacgcggtcg cctcggggac gacgcccact cgccccagca tcgccgcgtg ggccgccgag
     1441 ccctggaggt cgtcctcgaa caggcgagtg tcgaccgata gcgatgacag gaagcgttcc
     1501 acgcgcgggt cgagtccggt cggcgacatc tcccagagct tcagcacgcc gggcatccta
     1561 atatgccgcc gcgactttgg cggccttgag cccggagccg ccggagccgt tggcgcccgc
     1621 ggggacggcg cccgcgggga cggcgcccgc ggggacggcg cctttgcctg actcggcctc
     1681 caacctcgcc ttcatcaggc tcctggcctt gaccggtagg gagtataagt tgatgaagcc
     1741 ggtagcgtcg cgctggtcga acaatggccc ggtcgcgaag ctggccaacg agtggtcgta
     1801 cagcgagtac ggtgaactca taccggcaca ggacaggctt cctttccaga gcctcaactt
     1861 gacgacgccg ccgactgcct cctgggtgga gtcgacgaac gcattgaccg catccatcag
     1921 cggcgagtac cactcgccct cgtacatgag ctccgcgaag cgctgcgcga ccgtagcctt
     1981 gaacgacatg gtcttgcggt cgaggcagag ctgctccagc ttctggtgcg cctccatgag
     2041 gatcgccccg gccggcgtct cgtacacgcc gcggctcttc ataccgacga cccggttctc
     2101 gaccatgtcg agtatgccta tcccgtgccg gccgccgagc tcgttcagcc tggctacgag
     2161 cgacacgccg tccgtccgtt cgccgttcaa cgcgacgggt atgcccttct cgaaggtgag
     2221 ttccacgtac tcggcctcgt ccggcgcctt gtcgggcggc acgcacagct tgagcatgcc
     2281 ctcgaggacc ggctcgttca tcggatcctc gaggtcgagg ccctcgtggc tcacgtgcca
     2341 tagattgtcg tcgcggctat aggagtcgcc tttcttgaac ggcgtcggga tgccgtgcgc
     2401 ctccagatag gcaatctcct cctcgcggct acccaggtcc cagatccgcc acggcgcgat
     2461 gatctccatg tcgggagcga aggccttgag gcccatctcg aagcgtatct ggtcgttgcc
     2521 cttgccggtg cagccgtgcg ctaccgccga gcagccctcc actatagccg tctcggccag
     2581 ggccttggcg atcaggggcc tggccgcggc cgtgccgagc aggtaccgcc cctcgtacaa
     2641 agcaccagcc ttcacgagcg gccagagcgt ctcctcgacg aaggctttct tgaggtcgtt
     2701 cacgacgagc ttcgaagcgc cggacgaccg ggctttctgt tcgagcccgt cccagtcgca
     2761 ggcctgccct acatcgccgc agacggcgac gacgtcgcag tcgtagtgtt ccttaagcca
     2821 ggggacgatg acggtggtat cgagtccgcc cgagtaggcg agcgctatct tcttcattgg
     2881 ttactcctta cggttcgccc gaccggggct ccggtggccg aggtcaccga ggctccgccg
     2941 gaacgagcgg atacgcggcc gctcgtcgcc gcgtccatga cggggctatg acgatacggc
     3001 tatcctgctt cggagccata gcggccccga ccattcacat tcgattacgc tgtacgggaa
     3061 acggttggat taacgacgac gaaggcggag acggaggcgg ggagagacga aaggcgaaaa
     3121 agcggctacg acgggcgatg ataacgacga ttggctacta cacgggcctt gcggcgacca
     3181 aactactggt accttgttca tggtacatcc tccactcgtt tgttactccg aacaataacg
     3241 gcaaaaacga ttgcgcgtca agtactctca attcatgacg gcaagcgata cgaaagctag
     3301 ccgaatatgg cagaatatag catcgcacgg tataataccg cagacacgca tcgattctgt
     3361 agagtcctca agaaaagcag taccgaggta ttccgtctat gccacgaacg ataacggcac
     3421 agagttccgc caccacgatc gacgccccac ctgaggcgat ccaatatcgc gatcccgact
     3481 accagggcct cgaggccgcc ctcgaccgtc gagcggcggc cggggcgaac ccatcggaac
     3541 tcggcgcctt gatcgccaag gaatactcgc gccgcaaccg cgccatcaag gacccgggca
     3601 tccaccggct cgacccgtcg tccgtggccg acgactacct gtaccctcgt aaggacctca
     3661 accgcttcgc cgtcgagaac tggatccgcc gcgtcttcct cggcggactc aaaccgcgca
     3721 tcgtcggctc gagcctcgtc ttcggactcg gcaggctgtt ttcgacctac aacgacctcg
     3781 gggcccagca cgccaccgac gtcgacttga acattattgt cgacgacgcg ctgccggctc
     3841 ggttgtacgc ggagctgggc ggtaggctcg gtgacctgcg cgtcgagttg caccagcgct
     3901 ttggcatcct gctcgacctg caccctgact ataccctgct cagggagtcg gaggtgctcg
     3961 gggcgctgtc ggcggacgac gagacaaggc gctt
//
LOCUS       DAOPHV010000199         3977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_30883_length_3977_cov_3.699898, whole genome shotgun sequence.
ACCESSION   DAOPHV010000199 DAOPHV010000000
VERSION     DAOPHV010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30883_length_3977_cov_3.699898"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /gene="fabF"
                     /locus_tag="PLQ29_06405"
     CDS             complement(<1..1124)
                     /gene="fabF"
                     /locus_tag="PLQ29_06405"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPG86314.1"
                     /translation="MRKAVITGMGVVSPIGNDIDTFWASIKAGKSGLGPITRFDAAGF
                     DSRVAAEVKDFDPSLYMDRKDARKMALFTQFAVAAAVQAWRDAGLDGSSIDLERTAVM
                     LGNGIGGIEIFEQSHAKLLESGPSRMLPLTVPMMIGNEAAGNIAIHFGIKGPVQTAVT
                     ACASGSDAIGQALDMIRVGRADVVVAGGTEAAISAFAVGSFCMLKALSTAYNDDPAKA
                     SRPFDAARDGFVIGEGAGVMIVESEEHARARGARVIVELAGYGASCDAYHITAPEPSG
                     DGGARAIALALADAGIDPGQVAYYNAHGTSTPMNDPIETQMVKKAFGEHAYAMKVSST
                     KSMTGHMIAAAGAVEAIVCALAIRDGFFPPTINLETPDEA"
     gene            complement(1151..2137)
                     /locus_tag="PLQ29_06410"
     CDS             complement(1151..2137)
                     /locus_tag="PLQ29_06410"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPG86315.1"
                     /translation="MAITIAGTGASIPTNRVTNEELSKRVDTTDEWIRSHTGIGARHF
                     AADGSVTSDLAVEAARAAMAEAGKAPEDVDLIVVATATPDFFGFPSTACIVQDKLGAV
                     NAAALDINAGCTGFIYGLDVAGSMLASPSRKTALVIGAETLSRIADWSDRATCVLFGD
                     GAAAFVLEKTDAADCRGLLHSWLRAKGSGSRSLYLSQPERTKTFERNLDCGRPPAIMM
                     DGKAVYMFAVKAITDTIEALLAESGLPLADFRWIVPHQANARIVQAAAKRFGIADERV
                     YMNIEDFANTSAASIPIALNEMSRKGLLAQGDLVMAMGFGAGLTYGGVIIRW"
     gene            complement(2261..3013)
                     /locus_tag="PLQ29_06415"
     CDS             complement(2261..3013)
                     /locus_tag="PLQ29_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HPG86316.1"
                     /translation="MLSIRDLHKSFHRTEVLRGVDLDVAKGDVVAIIGPSGAGKTTLL
                     RCVNFLERADRGEMHFDGLHLDMRKASGKDMLAIRKRTAFVFQSYNLFNNKTALENVT
                     EGLVIGRGMPAREAEDVARRALDKVGLSNRLDYYPSKLSGGQQQRVGIARAVAVDPDV
                     ILFDEPTSALDPELIGEVLGIMRQLAREGTTMVVVTHELAFASDVANRVVFMDEGLVV
                     ESGPPDRVLYAPAEERTRQFLRRILPVGAPLG"
     gene            complement(3033..3746)
                     /locus_tag="PLQ29_06420"
     CDS             complement(3033..3746)
                     /locus_tag="PLQ29_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HPG86317.1"
                     /translation="MGLDLDYLARTLLLSLRGLPVTLGLCVATLVVSLPLGFMLAIVG
                     IYRVRVLRKLASVYISFARGTPIVLQILVVYSVLPSLANQVAIRAGWDVDVFALNPLW
                     YAVVVFSFNTTAVLSEIVRSAILTVDRGQYEAALSSGMTSRQAYRRIIVPQALLVALP
                     NLANATINLIKGTSLAFLMAVRDVTAIAKIEAARGYDYVESYLAVFIVYVFVCGATQA
                     LFTLIERRLGIYRAPVVAF"
     gene            complement(3761..>3977)
                     /locus_tag="PLQ29_06425"
     CDS             complement(3761..>3977)
                     /locus_tag="PLQ29_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86318.1"
                     /translation="VALLKEGSLAYTVGLVDLMGQGSLIIARGFGAWSLETYLAAALI
                     YWALTVAIEKAAGALERRLSVGRRGLA"
BASE COUNT          647 a         1258 c         1401 g          671 t
ORIGIN      
        1 caggcctcgt ccggcgtctc caggttgatg gtcggaggga agaagccgtc gcgtatggcc
       61 agggcgcaga cgatggcctc gacggcgcct gcggcggcta tcatgtggcc ggtcatgctc
      121 ttggtcgacg agaccttcat cgcgtaggcg tgctcgccga aggccttctt gaccatctgc
      181 gtctcgatgg ggtcgttcat cggggtgctg gtgccgtggg cgttgtagta ggccacctga
      241 ccagggtcga taccggcgtc ggcgagagcc agggcgatcg cccgggcgcc gccgtctccg
      301 gacggttcgg gggcggtgat gtggtaggcg tcgcagctgg cgccatagcc ggccagctcg
      361 acgatgacgc gggccccgcg cgcgcgggcg tgctcctcgc tctcgactat catcacgccg
      421 gcgccctcgc cgatcacgaa gccgtcgcgg gcggcgtcga aggggcgcga ggccttggcc
      481 gggtcgtcgt tgtacgcggt cgacagggcc ttgagcatac agaaggagcc gacggcgaag
      541 gcgctgatgg ctgcctcggt gccgccggcg acgacaacgt cggcgcggcc gacgcgtatc
      601 atgtccaggg cctggccgat ggcgtccgag ccggaggcgc aggcggtgac ggcggtctgg
      661 accggcccct tgatgccgaa atggatggct atgttgccgg cggcctcgtt ccctatcatc
      721 atcggcacgg tcaaggggag catccgcgac gggccggact cgagcagctt ggcgtggctc
      781 tgctcgaaga tctcgatgcc gcctatgccg ttcccgagca tcaccgcggt ccgctcgagg
      841 tcgatggacg agccgtcgag cccggcgtcc ctccaggctt ggacggccgc cgccaccgcg
      901 aactgggtga acagcgccat cttgcgcgcg tccttcctat ccatgtagag gctcgggtcg
      961 aagtccttga cctccgcggc caccctggag tcgaagcccg ccgcgtcgaa gcgcgtgatc
     1021 gggccaaggc cgctctttcc ggccttgatc gaggcccaga acgtatctat atcgttgccg
     1081 atggggctta cgacgcccat gcccgttatg acagccttgc gcatatcgct ctccttaccg
     1141 ttaacgccgc ttaccagcgg atgatgacgc cgccgtaggt cagaccggcg ccgaagccca
     1201 tcgccatcac gaggtcgccc tgggccagga ggcccttccg ggacatctcg ttcagcgcta
     1261 tgggaatcga cgccgccgag gtgttggcga agtcctctat gttcatgtag acgcgctcgt
     1321 cggcgatgcc gaaacgcttg gccgcggcct ggacgatgcg agcgttggcc tggtgcggta
     1381 cgatccaccg gaaatcggcg aggggcaggc cggattcggc gagcagggcc tcgatggtgt
     1441 cggtgatggc cttgacggcg aacatgtaga cggccttgcc gtccatcatg atggccggcg
     1501 ggcggccgca gtcgaggttg cgctcgaagg tcttggtccg ctccggctgg ctcaggtaga
     1561 ggctcctgga tccggagccc ttggcccgca gccaggagtg gaggaggccg cggcagtccg
     1621 cggcgtcggt cttctcgagc acgaaggcgg ccgcgccgtc gccgaagagt acgcaggtgg
     1681 cgcggtcgga ccagtcggct atacgcgaca gggtctcggc tccgatgacg agggccgtct
     1741 tgcgggatgg ggaggcgagc atcgagccgg cgacgtctag cccgtagatg aagccggtac
     1801 agccggcgtt tatatccaag gcggccgcgt tgacggcgcc aagcttgtcc tggacgatgc
     1861 aggcggtcga cgggaaaccg aagaagtcgg gcgtcgccgt cgcgacgacg atcaggtcga
     1921 cgtcctcggg cgccttgccc gcctcggcca tcgcggctcg cgcagcctcg acggccaggt
     1981 cgctcgtgac cgagccgtcg gcggcgaagt ggcgcgcgcc tatgcccgta tgggatcgta
     2041 tccactcgtc ggtcgtatcg acccgcttgg acagctcctc gttggtgacg cgattcgtcg
     2101 ggatcgacgc tccggtgccg gctatcgtta tggccatggt acgctccttg acataatcaa
     2161 gtcaaatgtc atgcgtatag tggcgtacca gacaaaagct gtcaatatgt aaattagaga
     2221 acgaatgaaa ccctggccta gcggctggga cggccgcgcg tcagccgagc ggagcgccca
     2281 ccggtaggat tcgcctcagg aactgcctcg tccgctcctc tgccggggcg tacaggacgc
     2341 ggtcaggcgg gccgctctcg acgacgaggc cctcgtccat gaacacgacc cggttggcca
     2401 cgtcggaagc gaaggccagc tcgtgggtga cgacgaccat ggtcgtgccc tcgcgggcca
     2461 gctgccgcat gatgccgagc acctcgccga tcagctcggg atcgagggcg ctcgtcggct
     2521 cgtcgaacag gatcacgtcg gggtcgacag cgacggcccg agcaatgccg acgcgctgct
     2581 gctggccgcc cgacagcttg gatgggtagt agtcgaggcg gttcgatagg ccgaccttgt
     2641 cgagggcccg cctggccacg tcctcggcct ctcgagccgg catgccgcga ccgatgacga
     2701 gcccctcggt gacgttctcg agcgcggtct tgttgttgaa caggttgtag ctctggaaga
     2761 cgaaggcggt ccgcttacgt atcgccagca tgtccttgcc gctggccttg cgcatgtcga
     2821 ggtggagtcc gtcgaagtgc atctcgccgc ggtcggcccg ctcgagaaag ttgacgcatc
     2881 gcagcagggt cgtcttgccg gcgcccgacg gcccgatgat cgcgacgacg tcgcccttgg
     2941 cgacgtccag gtcgacgcct cggagcacct cggtccgatg gaagctcttg tgcaggtctc
     3001 gtatgctcag catgccggct ctccgcgcct ttttagaacg ctacgacggg cgctcggtag
     3061 atgccgaggc gccgctcgat gagggtgaac agggcttgcg tggccccgca gacgaatacg
     3121 tatactatga agaccgccag gtacgactcg acgtagtcgt agccgcgcgc cgcctctatc
     3181 ttggcgatgg cggtcacgtc gcggacagcc atcaggaagg ccagcgaggt gcccttgatc
     3241 aggttaatcg tcgcgttggc caggttcggg agggcgacga gcagggcctg gggcacgatg
     3301 atgcgcctat aggcctggcg actcgtcatg ccgctcgata gcgccgcctc gtactggcca
     3361 cggtcgacgg tcaggatggc cgaccgtacg atctccgata gcaccgccgt ggtgttgaag
     3421 ctgaagacga ccacggcgta ccagagcggg ttcagcgcga agacgtcgac gtcccagccg
     3481 gcgcgtatgg cgacctggtt ggccaggctc ggtaggacgc tgtagaccac gagtatctgg
     3541 aggacgatgg gcgtgccgcg cgcgaaggag atgtagaccg aggcgagctt ccgtagcacg
     3601 cgcacccggt agatgccgac gatggcgagc atgaagccga gcggcagcga gacgacgagg
     3661 gtcgcgacgc agaggccgag cgtgaccggc agcccgcgga gcgatagtag cagggtgcgc
     3721 gccagatagt cgaggtcgag tcccatacga tgcgcggccc ctaggcgagg cccctccttc
     3781 cgaccgacag gcggcgctcc agggcgccgg cggctttctc tatggccacg gtcagcgccc
     3841 agtagatcag ggccgccgcg aggtaggtct cgagcgacca ggccccgaag ccgcgcgcta
     3901 tgatcagcga gccctggccc atcaggtcga ccaggccgac ggtgtaggcg agcgaaccct
     3961 ccttgagcag ggcgacc
//
LOCUS       DAOPHV010000200         3966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_30994_length_3966_cov_3.665814, whole genome shotgun sequence.
ACCESSION   DAOPHV010000200 DAOPHV010000000
VERSION     DAOPHV010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30994_length_3966_cov_3.665814"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PLQ29_06430"
     CDS             <1..413
                     /locus_tag="PLQ29_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPG86319.1"
                     /translation="GADLLDFGSGPEPVPAALLRERGYSVTAYDPYFAPGELWRSRSW
                     DAILVHEVAEHLAEPGETLAELAALLRPGGAWCIRTRFPPKDRESFARWRYRMDSTHV
                     GFFGPGSFAWLSARLGLEIALVEPPDRAVLVRPA"
     gene            complement(410..1015)
                     /locus_tag="PLQ29_06435"
     CDS             complement(410..1015)
                     /locus_tag="PLQ29_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPG86320.1"
                     /translation="MGRSGRAPTTRDLKARDTRERIYESAWSLIVEEGFDKVSVDRIC
                     AGAGVAKGSFYHHFRSKGDLIVEGYAMCDAYFDGEVAGRLEAPDAPGRIVEFVAHQMR
                     YAVRTGIDMMRQVYKSQLENGTRFFVSSERSLPRILRGIVVDGQAAGELRADLAADYV
                     VSFVLRFSRGTIYDWCLRDGSYDLEAVATEACARLVAVFKP"
     gene            1142..2434
                     /locus_tag="PLQ29_06440"
     CDS             1142..2434
                     /locus_tag="PLQ29_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HPG86321.1"
                     /translation="MYSPTMRLTEDERIILEGGRGEALRKAMESVVRFGDVFGADRLV
                     DLDHGVHLVTSFGIPLLGPVFDMMDELIAAGLETPEPFTVDPRPYDFANVPVGPIERL
                     VFTKVMYAKQKDYEAQLRKVGLKDGRAFTCACYFDEVGNVPKRGANLAWAESSAVVYA
                     NSVLGARSNRNSGVLELMCGIVGKAPRFGFLTDEGRQASWIVELKTTGLPEAQLLGSA
                     IGMQVVEDVPYIRGLDRYLGGTLDDRAKDYLKDMGAASASNGAVGLYHVDGLTPEAKD
                     LGEALVRKDARVYVIDDAEIERVKAGYPMMWKNADSKPTRCFIGCPHLSRAQLYGWLE
                     RLEASLSAAGRKRVAVPTVLTTAPDVAAAFRADAAAAARLEATGATLSSICPLMYMNN
                     PLCGKKPVITNSNKLRTYTKARYYTDADILAMVTGGRA"
     gene            2431..2880
                     /locus_tag="PLQ29_06445"
     CDS             2431..2880
                     /locus_tag="PLQ29_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HPG86322.1"
                     /translation="MKKSFKGRAVVPGTASGEAVVSRRGFNILASMQRDLMGKKAEVR
                     CSDQNNPDAFGRALSGRILCLPRTIGSTTGGMVLQAAAALGKAPAALLFSEPIDSLAA
                     GGVILAKVWNGRDIVTVDGLGDEFLAAVENGAAISVEADGIVTIETA"
     gene            2892..>3966
                     /locus_tag="PLQ29_06450"
     CDS             2892..>3966
                     /locus_tag="PLQ29_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoate reductase"
                     /protein_id="HPG86323.1"
                     /translation="MKTTHERVAELVESVGTWYEKPGCLASGRMTAELYPYTSMFSPI
                     RVNRLTIKNRLVMAPMGNVSMADETGRPSAKMIEYFTARARGGVGLITSGLVPVSHGV
                     DPTVTELGDLSYFPRIDRSRSVFAGWRDLAERCHAHGARFFIQLTPGLGRVGSPECLI
                     NKKKLPVSASWNPNFYMPALPCRPLTDGQARRIIKNSGQAAADAKAATIDGVYLHGHE
                     GYLLEQMTNPAFNRRKLGAFADWQAFGVKMIEEIRRRVGPDYPIMYRIDLSLALEETY
                     GERMDAVPSLKKFKGGRTPEQTLEYMESLVKAGVDLFDVDLGCYDNWWLPHPPSSMAP
                     GCYLEVSRIVKERFAERKLLSNAG"
BASE COUNT          684 a         1393 c         1267 g          622 t
ORIGIN      
        1 ccggcgccga cctgctcgac ttcggctcgg ggcccgagcc cgtgccggcc gccctgctcc
       61 gcgagcgggg ctactcggtg accgcctacg acccgtactt tgccccgggc gagctctggc
      121 gttcgcggtc ctgggacgcc atcctggtac acgaggtcgc cgaacacctc gcggagcccg
      181 gggagacgct ggccgagctc gcggccctac tccgcccggg cggcgcctgg tgcatacgca
      241 cccggtttcc gcccaaggac cgcgagtcgt tcgcgcgctg gcgctatcgg atggactcga
      301 cccatgtcgg gttcttcggc cccggatcat tcgcatggct gtccgcgcgc ctcggcctcg
      361 agatagccct cgtcgagccg cccgaccgag cggtactcgt gcgcccggcc taaggcttga
      421 agacggcgac gagccgggcg caggcctcgg tggccaccgc ctcgaggtcg taggagccgt
      481 cgcgcaggca ccaatcgtag atggtgcccc gcgagaagcg cagcacgaag gagaccacgt
      541 agtcagcggc caggtcggcc cgcagctcgc ctgcggcctg accgtctacc acgatgcccc
      601 ggagtatcct cggcagggac cgctccgacg atacgaagaa gcgcgtgccg ttctcgagct
      661 ggctcttata gacctggcgc atcatgtcga tgccggtccg gacggcgtag cgcatctgat
      721 gggccacgaa ctcgacgatg cggccgggtg cgtccggcgc ctcgagtcgg ccggccacct
      781 cgccgtcgaa gtaggcgtcg cacatggcgt agccctcgac gatcaggtcg cccttgctcc
      841 tgaagtggtg gtagaacgag cccttcgcga cgccggcccc ggcgcagatg cggtccaccg
      901 acaccttgtc gaagccctcc tcgacgatga ggctccacgc cgactcgtag atgcgctcgc
      961 gcgtatcccg cgccttgaga tccctcgtcg tcggagcccg gcctgatctt cccatgcgat
     1021 cactgtaccc gacattccgg cgcttgcgct atggcagaaa cggggctaca ctcattttgc
     1081 cgaccgcagt cggtatattt gtatttattt tcatcataca gcgtcccgaa aggagcaagg
     1141 catgtactcc cccacgatgc gcctgaccga ggatgaacgc ataatcctcg agggcgggcg
     1201 cggcgaagcg ttacgcaagg cgatggaatc ggtcgtccgc ttcggagacg tcttcggcgc
     1261 cgaccgcctc gtcgacctgg accacggcgt ccacctggtc acgtccttcg gcattccgct
     1321 cctcggcccg gtcttcgaca tgatggacga actgatagcc gcggggctcg agacgccgga
     1381 gccgttcacc gtagacccga ggccctatga cttcgccaac gtaccggtcg ggccgatcga
     1441 gcggctcgtc ttcaccaagg tgatgtacgc caagcagaag gactacgagg cgcagctccg
     1501 taaggtcggc ctcaaggacg ggcgagcctt tacctgcgcg tgctacttcg acgaggtcgg
     1561 gaacgtaccg aagcgcggcg cgaacctcgc gtgggccgag tcgtcggccg tcgtctacgc
     1621 caactcggtg ctcggcgccc gcagcaaccg caactccggc gtcctcgagc tgatgtgcgg
     1681 cattgtcggc aaggcgcccc gcttcggctt tctgaccgac gagggccgcc aggcgtcctg
     1741 gatcgtcgaa ctcaagacga cgggcctgcc cgaggcccag ctcctgggca gcgccatcgg
     1801 catgcaggtc gtcgaggacg tgccgtacat ccgcggcctc gaccgctacc taggcggaac
     1861 cctcgacgac cgcgccaagg actacctcaa ggatatgggc gccgcgagcg cgtccaacgg
     1921 ggccgtcggc ctgtaccacg tcgacggcct gacgcccgag gccaaggacc tgggcgaggc
     1981 gctcgtgcgc aaagacgcca gagtctatgt gatagacgac gcggagatag agcgcgtcaa
     2041 ggccggctac cccatgatgt ggaagaacgc cgactcgaag cccacgcgct gcttcatagg
     2101 ctgtccacac ctgtcgcgcg cccagctcta cggctggctc gagcggctgg aggcctcgct
     2161 ctcggcggcg ggcaggaagc gcgtcgctgt gccgaccgtg ctgaccacag cgcccgacgt
     2221 ggcggcggcg ttccgcgccg acgccgcggc agccgcgagg ctcgaggcga ccggcgccac
     2281 gctgtcgtcg atatgcccgc tcatgtacat gaacaatccg ctgtgcggca agaagccggt
     2341 gatcaccaac tccaataagc tgcggaccta caccaaggcc cgctattata ccgacgccga
     2401 cattctggct atggtcacgg gaggccgggc atgaagaaga gcttcaaggg acgagccgtg
     2461 gtaccgggta ccgcgagcgg cgaggccgtc gtatccaggc ggggcttcaa catcctcgcg
     2521 tcgatgcagc gagacctgat gggcaagaag gcggaagtgc gctgctccga ccagaacaac
     2581 ccggacgcgt tcggccgggc gctatccggc aggatactgt gcctaccgcg gaccataggc
     2641 tcgacgaccg gcggcatggt gctgcaggcg gccgcggctc tcggcaaggc tccggccgcc
     2701 ctactcttct ccgagcctat agactcgctg gcagcgggcg gcgtcatact cgccaaggtc
     2761 tggaacggcc gcgacatcgt cacggtcgac ggcctgggcg acgagtttct ggcggccgtc
     2821 gagaacggcg ccgccatctc ggtcgaggcc gacggcatag tcaccataga aacggcataa
     2881 ggagcaacga tatgaagaca acgcatgaac gcgtcgccga gctcgtggag agcgtcggca
     2941 cctggtacga gaagcccggc tgcctggcct caggcaggat gaccgccgag ctctacccct
     3001 atacctccat gttctcgcct atccgcgtga accgcctgac gatcaagaac cgcctcgtga
     3061 tggccccgat gggcaacgtc agcatggccg acgagaccgg tcggccgagc gccaagatga
     3121 tcgagtactt cacggctcgc gcgcgcggcg gcgtcggcct catcacctcg ggcctcgtgc
     3181 ccgtcagcca cggcgtcgac ccgacggtta ccgagctcgg cgacctgtcg tacttcccgc
     3241 gcatcgaccg gagccgctcc gtgttcgccg gttggcgcga cctggccgaa cgatgccacg
     3301 cccacggcgc ccgcttcttc atccagctca cgcccggcct cggccgcgtc ggctcgcccg
     3361 agtgcctgat caataagaag aagctgccgg tatcggcgtc ctggaacccc aacttctaca
     3421 tgcccgcgct gccctgccgc cccctgaccg acggccaggc caggcgtatc atcaagaact
     3481 cgggccaggc cgcagcggac gccaaggccg cgaccatcga cggcgtctac ctccacggcc
     3541 acgagggcta cctcctcgag cagatgacca acccggcctt caaccgcagg aagctcggtg
     3601 ccttcgccga ctggcaggcc ttcggcgtca agatgatcga ggagatccgc cgccgcgtcg
     3661 gccccgacta ccccatcatg tatcgcatcg acctatcgct cgcgctcgag gagacctatg
     3721 gcgagcgcat ggacgccgtc ccgagcctca agaagttcaa gggcggccgc acgccggaac
     3781 agaccctcga gtacatggag tccctcgtga aggcgggcgt ggacctcttc gatgtcgacc
     3841 tcggctgcta cgataactgg tggctacccc acccgccttc gtcgatggcc cccggctgct
     3901 acctcgaggt gtcgcgcatc gtcaaggagc gcttcgccga gaggaagctg ctgtccaacg
     3961 ccggcc
//
LOCUS       DAOPHV010000201         3948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_31184_length_3948_cov_3.561778, whole genome shotgun sequence.
ACCESSION   DAOPHV010000201 DAOPHV010000000
VERSION     DAOPHV010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31184_length_3948_cov_3.561778"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLQ29_06455"
     CDS             complement(<1..430)
                     /locus_tag="PLQ29_06455"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPG86324.1"
                     /translation="MLKRIGIALILAVSLAAATTEAQDRVDLVVLLDSSQSMFQYYNQ
                     VVDFVLSQTVKEYMRFGDAFHLVSFSDTTQVEIAQVLRTEKDIRSAVARLYLLYPLGR
                     NTDLVMALRNVYQYVADLPEGSAKHILLITDGMHSPAAGTQ"
     gene            complement(484..3168)
                     /gene="greA"
                     /locus_tag="PLQ29_06460"
     CDS             complement(484..3168)
                     /gene="greA"
                     /locus_tag="PLQ29_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPG86325.1"
                     /translation="MADSPIAKKLQDMLTEEKWTRATLSGYTVNQIKELDGLFNEAAK
                     EKALDEIRTVCADHLGHSKNSIAALYLSGIISLSRQQIDDSNMISLINIFADNRRWNI
                     VEFICQRILEYGENRNALVRLAECYENDDKVEEMYAVWERLVRVDYEEADIVKLIAEK
                     KEKDGDAEGAVDYYRKALHRYINKGLYSNVKEIWNKLIELAPADIDFFNHVQRKIAKQ
                     ISEEKAAGLLTDLYKHYKAASDWDTAIPILKTIIDYDDKNPGVRKEIVECYKAKYAGH
                     SHVDEYIRLSNLAQSYRSIHEAIADFEKHISFDVGNYVYHRTWNIGRIRSIKGDEITI
                     DFAKKRDHRMGLKMAVDSLITLSKDHIWVLKAIWSKDKLHDTVKKDIPWALKTIIKSF
                     DNYADIKKLKAELVPSVLSASEWTSWSSKARDILKTDAMFGNAPESVNIYLVRDRPLS
                     FEEKIYNQFKAEKNFFARIQDLRDFLDKGDADSELFAEMLQFFTGYVRSYSQLTEQVV
                     ASYLFLKELAAGHPILKNVVTLGFTEMMADCEDIVDIYASLKDTALKAAFLQHIKNFM
                     PDWADIYVRLFPYALSRQMIDVMLSAGEVARLQGMVVSIIENYRDHREAFIWVAKNLW
                     DEDWIKELGLSYDKMLITLIHILDISFKEIENHRDTTDNRRINKQVDTILFKDGRLTS
                     YFKTADKDDIERIFALISDVKDLDPAVKLSLRKQIAESHPDFKFNDEAEKTVVSRGLM
                     VTASKYEEKNKLLSHIMEVDVPANQKEIAFALSLGDLRENAEYKAAKEKQDELNAKVG
                     KLKNEIERAQIFDAGSITTGKISFATEVLLHNDLDDADEIYRILGPWESDPSNHVISY
                     LSPLGKRLLNHKVGDKLSFVIHERKFSYTVKAITAIDL"
     gene            complement(3189..>3948)
                     /locus_tag="PLQ29_06465"
     CDS             complement(3189..>3948)
                     /locus_tag="PLQ29_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86326.1"
                     /translation="VAGVSDAFEVGEYVMARWKAFRLFLAKLGPARDRPVSAFKQFAF
                     GVALQKFDSLADDGENADPEVLLRVGKANKGHGDYEAAVARLEAAAKLKRDDAAILAE
                     LADAYALIDEQRASKALFREAFYINPQRIDVELLESSAFARLAARVAELGKDGIEAAE
                     WIPVMGELLGVFSVKRELKPVEAGKLKQSVYELETELQSDGSRRAILVPRLINRYFWL
                     IDHYMNRKEDKSKIDELLLKIKLLDPFVYKQYIA"
BASE COUNT          684 a         1085 c         1249 g          930 t
ORIGIN      
        1 actgggtgcc cgcggcgggg gagtgcatac cgtcggtgat caggagtatg tgcttcgcgc
       61 taccctcggg caggtcggcc acgtactggt agacgttccg gagggccatc acgaggtcgg
      121 tgttgcgccc gagcgggtac agcaggtaca gcctggcgac ggcgctcctg atatccttct
      181 cggttctcag gacctgggcg atctcgacct gggtagtatc ggagaaggat accaggtgga
      241 aggcatcgcc gaagcgcatg tactccttaa ccgtctgcga cagcacgaag tcgacgacct
      301 gattgtagta ctggaacatg ctctgcgacg aatcgagcag gacgaccagg tcgacgcgat
      361 cctgggcctc cgtggtagcc gcggccagag agacggccag gatgagcgct atcccgatac
      421 gttttagcat gatcgattcc ttcgcttggt cacgcgcccg gattcagcgg gcgcgtgacc
      481 gaattatagg tcgatcgcgg tgatcgcctt taccgtatac gagaatttcc tctcgtggat
      541 gacgaatgac agcttatcgc cgaccttgtg gttcaggagg cgcttgccga gcggggacag
      601 gtacgagatg acgtggttgc tcgggtcgga ttcccaggga ccgagtatcc tgtagatctc
      661 gtcagcgtcg tcgaggtcgt tgtggagcag tacctcggtg gcgaaggata tcttgccagt
      721 cgtgatgcta cccgcatcga agatctgcgc ccgctcgatc tcgttcttga gtttgcctac
      781 cttggcattg agctcgtcct gtttctcctt ggcggccttg tactcggcgt tctcgcggag
      841 gtctccgagc gacagcgcga aggcgatctc cttctggttg gccggtacgt cgacctccat
      901 gatatgggac aggagcttgt tcttctcctc gtacttcgag gccgtgacca tgagtcccct
      961 cgaaacgacg gtcttctcgg cctcgtcgtt gaacttgaag tcggggtggc tctcggctat
     1021 ctgcttgcgt agcgatagct tgacggccgg gtcgagatcc ttgacgtcgc tgatcagggc
     1081 gaagatacgc tcgatgtcgt ccttgtcggc ggtcttgaag tagctcgtca ggcgaccgtc
     1141 cttgaacagg atggtatcga cctgcttgtt gatgcggcgg ttgtccgtcg tgtcgcggtg
     1201 gttctcgatc tccttgaagc tgatgtcgag gatatggatc agcgtgatca gcatcttgtc
     1261 gtacgagagc ccgagctctt tgatccagtc ctcgtcccag aggtttttag ctacccagat
     1321 gaaggcctcg cgatgatcac ggtagttctc aatgatggac acgaccatgc cctggagcct
     1381 agcgacctcg ccggcggaga gcatgacgtc gatcatctgg cgcgatagcg cgtaggggaa
     1441 cagccggacg tagatatcgg cccagtcggg catgaagttc ttgatgtgct ggaggaacgc
     1501 ggccttgagc gccgtgtcct tcaggctggc gtagatgtcg acgatatcct cgcagtcggc
     1561 catcatctcg gtaaagccga gcgtcaccac gttcttgagg atgggatggc cggcggccag
     1621 ttccttgagg aacaggtacg aggcgaccac ctgctcggta agctgggagt aggagcggac
     1681 gtagccggta aagaattgca gcatctcggc gaacagttcg gaatccgcgt cgcccttgtc
     1741 gaggaagtcc ctgaggtcct gtatcctggc gaagaagttc ttctcggcct tgaactggtt
     1801 gtagatcttc tcttcgaacg acagtggccg gtcgcggacc aggtagatgt tgaccgactc
     1861 gggcgcgttg ccaaacatgg cgtcggtctt caggatgtcg cgggccttgg aactccagct
     1921 ggtccactcg ctggcgctga gcaccgaggg cacgagctcg gccttgagct tcttgatgtc
     1981 ggcgtagttg tcgaagctct tgatgatggt cttgagggcc cagggaatgt ccttcttgac
     2041 cgtgtcatgg agcttgtcct tggaccagat agccttgagc acccagatat ggtccttgct
     2101 cagcgtgatc aggctgtcga cggccatctt gaggcccatg cggtggtcgc gctttttagc
     2161 gaaatcgatg gttatctcgt cgcccttgat gctgcgtatg cgcccgatgt tccaggtccg
     2221 gtgatagacg tagttgccga cgtcgaacga gatgtgcttc tcgaagtcgg cgatggcctc
     2281 gtggatgctg cggtatgact gggccaggtt ggacagcctg atgtactcgt cgacgtggga
     2341 atggccggcg tacttggcct tgtagcactc gacgatttcc ttgcgtacgc cggggttctt
     2401 gtcgtcgtag tctatgatgg tcttgagaat gggtatggcc gtatcccaat cggaggcggc
     2461 cttgtagtgc ttgtagaggt cagtcaggag gccggcggcc ttctcctcgc tgatctgctt
     2521 ggcgatcttg cgctggacgt ggttgaagaa gtctatgtcg gccggcgcga gctcgatcag
     2581 cttgttccag atctccttga cgttggagta caggcccttg ttgatgtagc ggtggagcgc
     2641 cttgcggtaa taatcgacgg cgccctcggc atcgccgtcc ttctccttct tctcggcgat
     2701 cagcttgacg atgtcggctt cttcgtagtc gacgcggacg agacgctccc agacggcgta
     2761 catctcctcg actttgtcgt cgttctcgta gcactcggcg aggcggacga gggcgttacg
     2821 gttctctcca tactcgagga tgcgctggca tataaactcg acgatgttcc agcggcggtt
     2881 gtcggcgaag atgttgatca gcgagatcat gttggagtcg tcgatctgct ggcgcgacaa
     2941 agagattata ccggagaggt agagcgcggc gatgctgttc ttgctgtggc cgaggtggtc
     3001 cgcgcagacc gtcctgatct cgtcgagggc tttttccttg gccgcttcgt tgaagaggcc
     3061 atcgagttcc ttgatctggt tgacggtata tccgctcagg gtggcccgcg tccacttttc
     3121 ctcggtgagc atgtcctgaa gctttttagc tatcggtgaa tcggccatct cggtctccta
     3181 tcctggaatc aggctatgta ttgcttgtaa acgaagggat ccaacagctt tattttaagg
     3241 aggagttcgt ctatcttcga cttatcctct ttgcggttca tatagtggtc tatcagccag
     3301 aaatagcgat tgatgagcct gggcaccagg atggcgcgcc tggagccgtc ggactgaagc
     3361 tccgtctcca gctcatagac tgattgctta agcttacccg cctcgacagg cttgagttcg
     3421 cgcttgaccg agaacacccc gagcagctcg cccatgacag ggatccattc ggccgcctcg
     3481 atgccgtcct tgccgagctc ggctaccctg gcggcgagac gtgcgaaggc gctcgactcg
     3541 agcagctcga cgtctatgcg ctggggattg atgtagaagg cctccctgaa cagggccttc
     3601 gaggcgcgct gctcatcgat cagggcgtag gcgtcggcta gctcggcgag gatggccgcg
     3661 tcgtcgcgct taagcttcgc cgcggcctcg agccgcgcca cggccgcctc gtagtcgcca
     3721 tggcccttgt tcgccttacc gaccctgagt agcacctccg ggtcggcgtt ctcgccgtcg
     3781 tcggccaggc tatcgaattt ctggagcgcc acgccgaagg cgaactgctt gaaggccgag
     3841 acggggcgat cgcgagcggg cccgagcttg gctaggaaga gcctgaaagc cttccagcga
     3901 gccatgacgt actctccgac ttcgaacgcg tcggagacgc cggcgacc
//
LOCUS       DAOPHV010000202         3937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_31302_length_3937_cov_3.865018, whole genome shotgun sequence.
ACCESSION   DAOPHV010000202 DAOPHV010000000
VERSION     DAOPHV010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31302_length_3937_cov_3.865018"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..739)
                     /locus_tag="PLQ29_06470"
     CDS             complement(197..739)
                     /locus_tag="PLQ29_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86327.1"
                     /translation="MKTRAICLALALAMVAGAAHGQAAPAEQPVPLFSDAGLMTGLIL
                     EATGFGLVIGGGATASLSLDAALVMMQIAPICSSVGAWVSQGKMEGYTELWQGRGLEY
                     DAIGLIKKSKTAAVATTAFTAGALVLPLLGEVGVYLSLACTGVAVGWDMYAFYAVRSI
                     WLRELNTAIVDSGMDWKAFR"
     gene            836..1567
                     /locus_tag="PLQ29_06475"
     CDS             836..1567
                     /locus_tag="PLQ29_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPG86328.1"
                     /translation="MDEMSWKAVHECSLEAGCAPTREGLISVFMAGAERLAPCDAGVG
                     VFDRELRCVACVGWDERTRRDYNQYYRGVVPFIGYDAIRYARRGQDVVRWVDYGETEF
                     MRDFGRALDLAYGLSPFRPTLPLNLSVQRSRRGPAFSADDCEALDALNAQMNNLLAVA
                     ERLGRAEYAPWTAERVRDALPDLSRREAEVLALCAEPLSMAQVASALAIGRRTAETHL
                     AHVFEKLGVRSRREALDALWLRVPR"
     gene            1578..2486
                     /locus_tag="PLQ29_06480"
     CDS             1578..2486
                     /locus_tag="PLQ29_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPG86329.1"
                     /translation="MRVAATQLLLAQGDVEGNCERAIRLVRLAARERAEVVVLPEFFT
                     SGVAIDDGMLAVPRLNREARVLERLRAEARGLGIIISGSMLCIWDADVYNSMVVVHPD
                     GSTFVHDKDLPTQFENAYYAPGDERRTDGAFALALCWELLRRKTVAEIGPSASLVLAG
                     SCWWSLPVGTSNQELDEYNDELNASTPAAFAAAAGLPVVHASLVGRIRTRRSLARDEV
                     VERRLVGTTQIVDRYGRVVASIRDKGSDDLIVEDVELAPRGSRRDPGAGFWLATMRDE
                     YLRAWETEGRLGRELYDVNRARMLGT"
     gene            complement(2521..2655)
                     /locus_tag="PLQ29_06485"
     CDS             complement(2521..2655)
                     /locus_tag="PLQ29_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86330.1"
                     /translation="MILGLPWWSWALILVGLALFVPLKTYFVKKFLAKKPEPPEDEES
                     "
     gene            complement(2666..3649)
                     /locus_tag="PLQ29_06490"
     CDS             complement(2666..3649)
                     /locus_tag="PLQ29_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG86331.1"
                     /translation="MRTNRSAWPGARKPGPLRAAVSIAAYDARRSARDMIVLYIIASP
                     LLLALIARFVLPLLAPPPMTVYLAPDVPVQVAVLLGEAGHGIAASPDTADQLLAADPG
                     TPAGLLATVAAGRDDAADLLRAALAGEFRHTRPALADTVAAFLMLSAAFLAALGSGLS
                     IVEERSAGVDRALAVSPVGVGTYFAGKLLFIATVSIAVSLGASLLLYGLDAPFGKLTL
                     AALAAAPLGLAAALALGLMAGSMIAAMGAIKLIMPLFLTVPLVYVIVPPGARAWFLAF
                     PNVWMLELYRATFAGAKHSGYLVPLAATAVLGGALLIPLSAIARKKLGLKL"
     gene            complement(3646..>3937)
                     /locus_tag="PLQ29_06495"
     CDS             complement(3646..>3937)
                     /locus_tag="PLQ29_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86332.1"
                     /translation="LGLTIAVFSGGMSGWFIPAVGILMVNMAPMGLADGYPVAYWLTP
                     GGAALAGYRSLFGMGRPGMTGASILWLGTAAVVVLPLSWWAVRTRVFKEGRR"
BASE COUNT          670 a         1325 c         1341 g          601 t
ORIGIN      
        1 cccgcgctac ggcccacgtc gaggcgttcg atcaggtaca gctcgcaggt gaccgatgcg
       61 tcgtagtcgc cgtcgaccga aacgtaaagc tcggcggtct cctggaacag gagctgacag
      121 ccgcagaacg acatggccag ggcgacaagg agcgctagaa cgatcggttt cctcatcttc
      181 ggcaccctcg acccgctcaa cggaaggcct tccagtccat gcccgaatcg acgatggccg
      241 tattgagctc gcgtagccag atgctccgca ccgcgtagaa ggcgtacatg tcccagccta
      301 ccgccacgcc ggtgcaggcc agcgataggt agacgcccac ctcgccgagc aacggcaaca
      361 ccagggcgcc ggccgtgaag gcggtggtcg cgacggcggc cgtcttggac ttcttgatca
      421 ggccgatcgc gtcgtactcg aggcctcgtc cctgccatag ttcggtgtag ccctccatct
      481 tgccctgcga gacccaggcc cctaccgagg agcagatggg cgctatctgc atcatcacga
      541 gggccgcgtc gagcgatagc gacgcggtgg cgccgccgcc tatcacgagg ccgaagccgg
      601 tagcctcgag gataagtccg gtcatcaggc cggcgtcgga gaagagcggt accggctgtt
      661 cggccggcgc ggcctgcccg tgggcggctc cggctaccat agcgagcgcc agggccaagc
      721 atatcgcgcg ggttttcatg gagacctcca aacatggata taccgtaacg ctagcctccc
      781 gattggtttg gcggtatccg tatattttac gtattcatgc gctatactcg aggccatgga
      841 cgaaatgtca tggaaggcgg tgcacgagtg ctcgctcgag gccggctgcg ccccgacgcg
      901 cgagggcttg atctcggttt tcatggccgg ggcggagcgg ctcgccccct gcgacgccgg
      961 cgtcggcgtc ttcgatcggg aactgcgctg cgtcgcctgc gtcggctggg acgagcggac
     1021 caggcgcgat tataaccagt actaccgcgg cgtcgtgccg ttcatcgggt acgacgcgat
     1081 ccgctacgcc aggcgcggcc aggatgtcgt ccgctgggtc gactatggcg agaccgagtt
     1141 catgcgcgat ttcggtcggg ccttggacct ggcctacggc ctgagcccgt tcaggccgac
     1201 cctgccgctc aacctgtcgg tccagcgctc gcgccgcgga ccggccttca gcgccgacga
     1261 ctgcgaggcc ctggacgcgc tcaacgcgca gatgaacaac ctgctcgccg tggcggagcg
     1321 gctcgggcgg gccgagtacg cgccatggac cgcggagcgc gtacgcgacg ccctgcccga
     1381 cctgagccgc cgcgaggccg aggtgctcgc cctgtgcgcc gagcccctgt cgatggccca
     1441 ggtagcctcc gcccttgcga tcggccgccg tacggccgag acccatctgg cccacgtctt
     1501 cgagaagcta ggcgtccgct cgcgccgcga ggcgcttgac gcgctctggc tgagggtccc
     1561 gaggtgaggg cgtacgcatg agggtggcgg cgacgcaact gctgttggcc cagggcgacg
     1621 tcgagggcaa ctgcgagcgg gcgatacgcc tcgtgaggct ggctgcccgc gagcgtgctg
     1681 aagtcgtcgt cctgcccgag ttcttcacct cgggcgtagc catcgacgac ggcatgctcg
     1741 ccgtgccgag gctgaaccgc gaggcgcggg tgctcgagcg cctgcgggcg gaggcccggg
     1801 gcctcggtat catcatatcc gggtcgatgc tgtgtatctg ggacgcggac gtctacaact
     1861 cgatggtcgt cgtccatccc gacggatcga ccttcgtcca cgacaaggac ctgccgaccc
     1921 agttcgagaa cgcctactac gcgccgggcg acgaacggcg gaccgacggc gccttcgcgc
     1981 tcgcgctgtg ctgggagttg ctgcggcgta agaccgtcgc cgagatcggg ccgagtgcga
     2041 gccttgtgct cgccgggtcc tgctggtgga gcctgcctgt cggcacctcg aaccaggagc
     2101 tcgacgagta taacgacgag ctgaacgcgt cgacgcccgc cgccttcgct gccgcggctg
     2161 gcttgcccgt cgtccacgcg tcgctcgtcg gccggatcag gaccaggcgc tccctcgcgc
     2221 gcgacgaggt cgtggaacgt cgcctggtcg gcacgaccca gatagtcgac cggtacggtc
     2281 gagtcgtcgc gtcgatacgg gacaagggct cggatgacct gatcgtggag gatgtcgagt
     2341 tggcaccccg cggttcacgg agggatcccg gcgccggctt ctggctggct acgatgcgcg
     2401 acgagtacct gagggcctgg gagaccgagg gtcggctcgg ccgggagctg tacgacgtga
     2461 atcgcgcgcg catgctcggg acgtagccgg atccccgatc cgccgcgcga cctggaagta
     2521 tcagctctcc tcgtcctccg gcggttccgg cttcttggcc aggaacttct tgacgaagta
     2581 cgtcttgagc ggcacgaaaa gggccagccc gacgaggatg agcgcccagg accaccacgg
     2641 taaaccaaga atcatatcga cctccttata gcttcaagcc gagcttctta cgggcgatcg
     2701 ctgaaagcgg gatcaacagg gcgccaccca ggacggcggt cgccgcgagc ggaaccaggt
     2761 atccagaatg tttggcgccg gcgaacgtcg cccggtacag ttcgagcatc cagacgtttg
     2821 ggaacgcgag gaaccacgcg cgcgcgcctg gcggcacgat gacgtagacc agcggcactg
     2881 tcaggaataa cggcatgatc agcttgatgg cgcccatggc cgcgatcatc gagccggcca
     2941 tcaggcccag ggccagcgcc gcggccagcc cgagcggcgc cgccgccaag gcggccagcg
     3001 tcagcttgcc gaagggcgca tccaggccgt acaacaggag cgaggcgccg agcgataccg
     3061 cgatcgatac cgtagctatg aatagcagct tgccggcgaa ataggtaccc acaccgactg
     3121 gagacacggc caaggcgcgg tcgacgccgg cgctacgctc ctcgacgatc gacagccccg
     3181 agccgagcgc cgccaggaac gccgccgaca gcatcaggaa ggccgcgacc gtgtcggcta
     3241 gggccggtcg cgtatgcctg aattcaccag cgagtgccgc gcggagcagg tccgcggcgt
     3301 catctcgtcc cgccgcgacc gtggccagga gcccggccgg cgtgccagga tcggccgcca
     3361 ggagctggtc cgcggtgtcg ggcgacgcgg ctatgccgtg gccggcttcg ccgaggagga
     3421 ctgccacctg aaccggcacg tccggcgcca gatacacggt catcggaggg ggcgcgagca
     3481 gcggcaggac gaacctggct atgagcgcca ggagtagcgg gctggctatg atgtacagca
     3541 ctatcatatc tcgggcgctg cgcctggcgt cgtacgccgc tatggagacc gcggcccgta
     3601 gcgggccggg cttgcgcgcg ccgggccatg cggatcgatt cgtcctcatc gtcggccctc
     3661 cttgaacacg cgcgtacgca ccgcccacca ggacaggggc agcacgacga ctgcggcggt
     3721 gccgagccat aggatggagg cgcccgtcat gcccggccgc cccatgccga acaggcttct
     3781 ataccccgcg agagccgctc cgcccggcgt tagccagtac gctacggggt agccgtcggc
     3841 caggcccatc ggcgccatgt tcaccatcag gatcccgacc gccggaatga accatcccga
     3901 cataccgccg gagaatacgg cgatcgtcag gcccagc
//
LOCUS       DAOPHV010000203         3935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_31333_length_3935_cov_3.575515, whole genome shotgun sequence.
ACCESSION   DAOPHV010000203 DAOPHV010000000
VERSION     DAOPHV010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31333_length_3935_cov_3.575515"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..645
                     /locus_tag="PLQ29_06500"
     CDS             112..645
                     /locus_tag="PLQ29_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86333.1"
                     /translation="MSKAALSVAAFAVFALFPGIAAAAPNDPPAETLAIVFDLQSLVS
                     VASPYDDGYQSGVGCKWWPKDSLAVRALVGLELNAQDGQTTTELGVSGAAEWHPSRRP
                     TVSPYAGGLAGLRATMDGENRVDLYLGGLAGAELRLFGPVALYAEYRLLAALDSGGFT
                     LSLGGAGSAQLGLLFYL"
     gene            659..1519
                     /locus_tag="PLQ29_06505"
     CDS             659..1519
                     /locus_tag="PLQ29_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86334.1"
                     /translation="MSIRRTTIARAIMIATALISVACDWEIGTDLPNTVTPGGATGRR
                     TTLIDHRSATIDGLSDAELAAARALRVAYWHTSHGSQLVTGNDGMDAFYGGRGRFVLG
                     GQDGLALDEQGTDIGNPGLEDFDASVRAYLADHADTDVVMASWCGQVSGASEAMIDRY
                     LEIMGAFETEFPSVDFVYMTGHADGSGLSGNLHIRNQQVREHCVANGSWLYDFYDIEC
                     YDPDGTYYGDRNVDDACGYDGGNWARAWQDAHPGQWWECSSAHSEPLNANQKAKAAWR
                     LWALLAAARS"
     gene            1608..2204
                     /locus_tag="PLQ29_06510"
     CDS             1608..2204
                     /locus_tag="PLQ29_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPG86335.1"
                     /translation="MPKIVDYDARRKDIADKTVSVLVRDGFRELKLGKVAALCGIGRT
                     TIYQYFRNVGQIIDYALADVFKRLDAEIAAAAADDSLAPSGKLLRVMEFLERVAILDK
                     ERMVLVLDFLLHPKREVPGVKFDVQEHVRALRVGLERVLQAGVDSGELRPMEAKSMAF
                     TLFSFVEAATVHSALYDNISLDTTLRDLRILLDGLKAR"
     gene            complement(2395..3783)
                     /locus_tag="PLQ29_06515"
     CDS             complement(2395..3783)
                     /locus_tag="PLQ29_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPG86336.1"
                     /translation="MTDVNVARVVIGLVVAMAIIIVTVTKTKIHVFPAMLIGAVAAGL
                     VAGMPSADVMSNIISGFGGTIGSIGIIIGFGVMMGAIFEASGAAKRMALTFIKLLGKG
                     KEDIAMGITGFIVSIPVFCDSAFVVLSPLAKSLARNTGKSLITISCALGTALLITHSL
                     VPPTPGPLAVVNYFKLDLGMFMIYSLIASALIVPFGLIYARWVGKKIFRLPDPANPGE
                     LIDRPYTASELESPEQLSHEKLPGTFISFAPIVIPIALILFNTVSSVLGASGLLKSVA
                     TLIGHPVVAVGIGLLIAIYGLAGKTDRKEVVALLDKAMQDAGIIVFVTGAGGSLGQVL
                     KATGAGGSIAELLVAAHIPGILLPYIMAFILRIVSGSATVGMLTAGSITAPILLSMAG
                     VNPYMGALACCIGSIGITGLHDSYFWVVNRTTGLKDMKDMLWHWFGAVLALSVGGAIV
                     LFVMNFVVGKIA"
BASE COUNT          698 a         1302 c         1294 g          641 t
ORIGIN      
        1 gctacgcgcc cttccctccc ggccccttcc atccccgccc cttccatccc cgcccgagcg
       61 cgctatactc tggtaacgta gcgagacctc atcgcgcaga agagtgtacc catgagtaaa
      121 gccgcgttat cggtcgccgc gttcgccgtc ttcgccctgt tccccggcat cgccgcggcc
      181 gccccgaacg acccgcccgc cgagacgctc gcgatcgtct tcgacctcca gagcctcgta
      241 tcggtggcgt cgccctacga cgacggctac cagagcggcg tcgggtgcaa gtggtggccc
      301 aaggactcgc tggccgtcag ggccctcgtc ggcctcgagc tgaacgccca ggacgggcaa
      361 acgacgacgg agctcggcgt ttcaggagcc gccgagtggc atccgtcgcg gcggccgacc
      421 gtctccccgt acgccggcgg gctggccggc ctacgcgcga cgatggacgg cgagaaccgc
      481 gtcgacctct acctgggcgg cctcgccggg gcggagctga ggctgttcgg cccggtcgcc
      541 ctgtacgcgg agtaccgact cctcgcagcc ctcgactcgg gcggcttcac cctatcgctc
      601 ggcggcgccg gctccgccca gctcggcctc ttgttctatc tctgagcggg gagggaccat
      661 gtcaatacgc cgaacaacca tcgcccgggc catcatgatc gcgacggcgc tcatatcagt
      721 cgcctgcgat tgggaaatag gcaccgacct accgaatacc gttaccccag gcggcgccac
      781 gggtcggcgc acgaccctca tcgaccaccg ctccgcgacg atcgacggcc tatcggacgc
      841 cgagctcgcg gccgccaggg ccctgcgcgt ggcgtactgg cacacctcgc acggcagtca
      901 actcgtcacc gggaacgacg gcatggacgc gttctacggc ggccggggcc gcttcgtcct
      961 cggcggccag gacggcctgg cgctcgacga gcagggtacc gacataggca acccggggct
     1021 cgaggatttc gacgcgtcgg tccgggccta cctggccgac cacgccgaca ccgacgtcgt
     1081 gatggcctcc tggtgcggtc aggtctcggg cgccagcgag gcgatgatag accgctacct
     1141 agagatcatg ggcgccttcg agaccgagtt tccctcggtc gacttcgtct acatgaccgg
     1201 ccacgccgac ggctccggcc tctcgggcaa cctgcatatc cgcaaccagc aggttcgaga
     1261 gcattgcgtg gccaacggta gctggctcta cgacttctac gacatcgaat gctacgaccc
     1321 cgacggaacc tactacggcg accggaacgt ggacgacgcc tgcggctacg acggcggcaa
     1381 ctgggcgcga gcctggcagg acgcgcatcc gggccagtgg tgggaatgtt cctcggccca
     1441 ctcggaaccg ctcaacgcca accagaaagc caaggccgcc tggcggctct gggcgctcct
     1501 ggccgctgcc cgctcgtaag gctggtccgc ggcctcgggt tcgaagcgac ggcggttgat
     1561 cggcgcatcg tatggaccct ataatggcgt cgccatgagg agaaacgatg cctaaaatcg
     1621 tcgactacga cgcgcgccgc aaggatattg cagataagac cgtctccgtg ctggtgcgcg
     1681 acggcttcag ggaactcaag ctcggcaagg tcgccgcgct gtgcggcatc ggccggacga
     1741 ccatctacca atattttagg aacgtaggcc agatcatcga ctacgcgctc gccgacgtct
     1801 tcaagcggct cgacgcggag atcgccgccg ccgcggcgga cgactcgctc gcgccgtcgg
     1861 gtaagttgct ccgcgtcatg gaattcctcg agcgggtcgc catcctcgac aaggagcgca
     1921 tggtcctcgt gctcgacttc ctgctccacc ccaagcggga ggtaccaggc gtcaagttcg
     1981 acgtgcagga gcacgtccgg gccctgcgcg tcgggctcga gagggtgctc caggccggcg
     2041 tcgattccgg cgagctccgc cccatggagg ccaagtccat ggccttcacg ctcttctcgt
     2101 tcgtcgaggc ggctaccgtg catagcgcgc tgtacgacaa catatcgctc gatacgacgc
     2161 tgcgcgacct acgcatcctg ctcgacgggc ttaaggcgcg gtaagcgacc cgacgagcga
     2221 gcctcgctcc accgccagca tcgtccgtcc cacacgccag gtggcggcta tccgggaccg
     2281 ggcggtacgc ccctcgtgcg tcggccagga gctgtcgtcg aacacggaaa ggacccgccg
     2341 agcttagccc ggcgggtccc atggtcgatc gatccgtagg cctggatcgt accgttaggc
     2401 gatcttgccc acgacgaagt tcatcacgaa caggacgatg gctccgccga cggacagcgc
     2461 gaggaccgcg ccgaaccaat gccagagcat gtccttcatg tccttgaggc cggtcgtgcg
     2521 gttgacgacc cagaagtagc tgtcatggag accggtgata ccgatcgagc cgatacagca
     2581 ggcgagcgcg cccatgtacg ggttgacgcc ggccatcgac aggaggatcg gggcggtgat
     2641 cgagccggcg gtcagcatgc cgaccgtggc cgaaccggag acgatgcgca ggatgaaggc
     2701 catgatgtac ggcaggagga tgccggggat gtgggccgcg acgagcagct cggcgatcga
     2761 gccgcccgct ccggtggcct tgagcacctg gccgagcgat ccgccggcgc cggtcacgaa
     2821 cacgatgatg cccgcgtcct gcatggcctt gtcgagcagg gccacgactt ccttgcggtc
     2881 ggtcttgccg gccaggccat agatggctat caggaggccg atgccgacgg ctacgaccgg
     2941 gtggccaatc agcgtggcga cggacttgag caggccggag gccccgagga cgctggagac
     3001 cgtattgaag aggatcagcg cgatcggaat gacgatcggg gcgaacgaga tgaaggtgcc
     3061 cgggagcttc tcgtggctga gctgctcggg gctctcgagc tcggaggcgg tgtacggcct
     3121 atcgatcagc tcgccggggt tggccgggtc gggcaggcgg aagatcttct tgcctaccca
     3181 gcgggcatag atcaggccga acggcacgat cagcgctgac gcgatcaggc tgtagatcat
     3241 gaacatgccg aggtcgagct tgaagtagtt gaccacggcc agcggaccag gggtcggcgg
     3301 cacgagcgag tgcgtgatga ggagggcggt gccgagcgcg cagctgatgg taatcaggct
     3361 cttgccggta ttcctggcca gggacttggc caggggcgag agcaccacga aggcggagtc
     3421 gcagaagacc gggatggaca cgatgaagcc agtgatgccc atcgctatgt cttccttgcc
     3481 cttgcccagt agcttgatga aggttagggc catgcgcttg gcggcgcctg aagcctcgaa
     3541 gatcgcgccc atcatcacgc cgaagccgat gatgatgccg atgctgccga tggtcccgcc
     3601 gaagccgctg atgatgttgc tcatcacgtc ggcggacggc atgccggcca ccaggccggc
     3661 cgcgaccgcg ccgatcagca tggcggggaa tacgtggatc ttggtcttgg tcaccgtgac
     3721 gatgatgatc gccatggcga cgacgagacc gatgactact ctagcgacgt ttacgtcagt
     3781 catcctcatt cgctccttat gatggtgcta tacagccggg gccgacggcc ccggcgaaat
     3841 gtctgttaga gtaccggacc caagcagtaa aagtccatcg atttcgtata cttgatcgac
     3901 tcgcccttcg tcgtctcgcc ggaggcgacc cggac
//
LOCUS       DAOPHV010000204         3925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_31449_length_3925_cov_3.813695, whole genome shotgun sequence.
ACCESSION   DAOPHV010000204 DAOPHV010000000
VERSION     DAOPHV010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31449_length_3925_cov_3.813695"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLQ29_06520"
     CDS             complement(<1..326)
                     /locus_tag="PLQ29_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPG86337.1"
                     /translation="MTDAKKLRIDAWDIRPWLVEIRRALHRHPELGLEEHRTADFIEA
                     RLEEMGVERERRGTAVVGLVRGARPGRTVALRADIDALPIREETGAEYASEVDGVMHA
                     CGHDAHT"
     gene            complement(323..1588)
                     /gene="alr"
                     /locus_tag="PLQ29_06525"
     CDS             complement(323..1588)
                     /gene="alr"
                     /locus_tag="PLQ29_06525"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPG86338.1"
                     /translation="MLRSTRFDIDLDAIAHNLRQVKGMVGAPNDGPEGDDEAGRGAAF
                     TPAFTPVKIAAVVKADAYGLGAVRVARALLREGVDMLAVACLPEALRLRRAYPDAAIL
                     VMGHTPDEYLPVAARERVTCSLFDLGQAEAFSTAARAAGVRAVAHVKIDTGMNRLGLK
                     PGLFEPGIGAGSAAELLGRMASLPSLELEGIFTHLALYDAASDAAQFAAFERLVDEAA
                     SLGLRFAIRHVCDSIGLMRYPAYRLDMVRPGAVLYGVTPLDTPLTDSADMITPFRLST
                     RISRLRRLSPGEGVGYDFSYRAPPGGALLATLPVGYADGYKRCLSNVASVLVRGVRAP
                     VVGLICMDQCTVDVSAVPGVAEGDEVVLLGGDRRGEPGSGVPVLEVASWVGTNRNDVI
                     CSIGTRVPRAYYEGGELVAETDYVMEGLV"
     gene            complement(1582..2718)
                     /gene="alr"
                     /locus_tag="PLQ29_06530"
     CDS             complement(1582..2718)
                     /gene="alr"
                     /locus_tag="PLQ29_06530"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPG86339.1"
                     /translation="MIDRQAWAEVDLSAIRRNVKRLVALAGGDSGLCAVVKADAYGHG
                     AAPVARACLEAGATSLAVAMVDEGLALRAAGVVEPILVMGYTGPEGAGRAVAGGLSVA
                     VVSVENARMVAAQAELRGRVARLHLKVDTGMSRLGVPASEAAAAAKAIASTPGALLEG
                     VFSHFADADDPESVFARLQLERFNEATAAIEAAGVRGFARHIANSSAYMRMPASRLDM
                     GRVGIAVYGLGVAGLEADGPALVPAMALKAKVTQVRTLPAGDSVGYGRTFVARRASRI
                     GTLPLGYADGVSRALSNRGWVGLPSGKAPIVGTVCMDQFMVDLTDLPDEGEGATATLF
                     GPGGPSAGELAELLGTIDYEIISSVSVRVPRIYLDSRLEEEEPC"
     gene            2894..3652
                     /locus_tag="PLQ29_06535"
     CDS             2894..3652
                     /locus_tag="PLQ29_06535"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPG86340.1"
                     /translation="MRPLIGITSFDDQKTRSTYAAINANYLYSIDAAGGIAVVLPGPS
                     GLRTAGSFAEPGDDELAESAEAYAAGLDGLLLSGGGDVSPRLLGRSPSRGVARFDSDR
                     DRWDLALFAAARRRGLPVLGVCRGCQVVNVALGGGLHEDLPTDLPGAGGHSFDVPMDE
                     LAHAVDIEPGSRLASALGQTRLPVNSFHHQAASGAAPGLVVTARADDGVIEALESPAG
                     EPYLVAVQFHPEGLTRRYPAFLGIFRGFVETARR"
     gene            complement(3649..>3925)
                     /locus_tag="PLQ29_06540"
     CDS             complement(3649..>3925)
                     /locus_tag="PLQ29_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HPG86341.1"
                     /translation="IAVINKGEIVELAETEELFRNPLHPYTRALISAIPQPDPLVERK
                     KVVMVYDPKCHDCSTDKPAWTDVGGGHFVLANEKEADAYRARIASGP"
BASE COUNT          571 a         1437 c         1361 g          556 t
ORIGIN      
        1 gtatgggcgt cgtggccgca ggcgtgcatg acgccgtcga cctccgaggc gtactcggcg
       61 ccggtctcct cgcgtatagg cagcgcgtcg atgtcggccc gcagggcgac cgtccggccg
      121 ggcctggccc cgcggaccaa gccgacgacg gcggtgccgc gccgctcgcg ctcgaccccc
      181 atctcctcga gcctcgcctc gatgaaatcc gccgtccggt gctcctcgag gccgagctcc
      241 ggatgacggt gtagcgctcg cctgatctcg acgagccagg gccggatgtc ccaggcgtcg
      301 atgcgaagct tcttggcgtc ggtcatacca ggccctccat gacgtagtcg gtttcggcca
      361 cgagttcgcc gccttcgtag taggcgcggg gaaccctggt gccgatcgag cagatcacat
      421 cgttacggtt ggtgccgacc caggaggcca cctcgagcac cggcacgccg gatccgggct
      481 ccccacgtcg gtcgccgccg agcagcacga cctcgtcgcc ctcggccacg ccgggcacgg
      541 cgctgacgtc gaccgtgcac tggtccatgc agatcaggcc cacgaccggc gcccggacgc
      601 cccggaccag gacgctggcc acgttcgaca gacagcgctt gtaaccgtcg gcgtagccga
      661 ccggcaaggt ggccaggagg gcgccgcccg gaggcgcgcg atagctgaag tcatagccga
      721 cgccctcgcc cgggctcagg cgcctgagtc gagatatcct ggtgctcagg cggaaggggg
      781 tgatcatgtc ggccgagtcg gtcagcgggg tatcgagcgg ggtgacgccg tacagcacgg
      841 cgcctggcct caccatatcc aggcggtagg cggggtagcg catcaggccg atcgagtcgc
      901 agacgtgcct gatcgcgaag cgcaggccga gcgaggccgc ctcgtcgacg agccgctcga
      961 acgccgcgaa ctgggccgcg tcggaggccg cgtcgtagag cgccaagtgg gtaaagatac
     1021 cctcgagctc gagggacggc agggaggcca tgcggcccag gagctcggcc gccgagccgg
     1081 cccctatgcc cggctcgaac agcccgggct tgaggccgag gcggttcatg ccggtgtcga
     1141 ttttgacgtg ggcgaccgcc ctgacgccgg ccgcccgggc cgcggtcgag aaggcctcgg
     1201 cctgcccgag gtcgaacagg gagcaggtga cgcgctcgcg ggccgcgacc ggcaggtact
     1261 cgtcgggcgt gtggcccatc accaggatcg ccgcgtcggg ataggcccgt cggaggcgca
     1321 gggcctcggg caggcaggcg accgcgagca tgtcgacgcc ctcgcgcagg agggcgcggg
     1381 ctacgcgcac cgcaccgagg ccgtacgcgt cggccttgac gacggccgcg atctttaccg
     1441 gagtgaacgc cggagtgaac gcggcgcctc gtcccgcctc gtcgtcgccc tcggggccgt
     1501 cgttcggcgc gcctaccatg cccttgacct gcctgaggtt gtgggcgatg gcatcgaggt
     1561 cgatgtcgaa gcgggtagac ctcagcacgg ctcctcctct tccaggcggc tatccaggta
     1621 gatcctgggc acgcgcacgg agaccgagct gattatctcg tagtcgatgg tgccgagcag
     1681 ctccgccagc tccccggccg aagggccgcc cggcccgaac agggtcgcgg tcgcgccctc
     1741 gccctcgtcg ggcaggtcgg tcaggtcaac catgaactgg tccatgcaga ccgtgccgac
     1801 gatgggggcc ttcccggacg gcaagccgac ccagccgcgg ttggacaggg cccgcgacac
     1861 gccgtcggcg tagccgagcg gtaacgtgcc tatcctcgag gccctgcggg cgacgaaggt
     1921 gcggccgtag ccgacgctgt cacccgcggg cagcgtcctg acctgcgtga ccttggcttt
     1981 gagcgccatc gcgggaacca gggccggtcc gtcggcctcg agcccggcga cgccgagccc
     2041 gtagacggcg atgccgaccc gtcccatatc cagccgagag gcgggcatcc gcatatacgc
     2101 ggagctgttg gcgatgtgcc gcgcgaagcc gcgcaccccg gcggcctcga tggcggccgt
     2161 cgcctcgtta aagcgctcga gctggagccg cgcgaagacg ctctcggggt cgtcggcgtc
     2221 ggcgaagtgg gagaagacgc cctcgagcag ggcgccgggc gtcgaggcta tcgccttggc
     2281 cgcggcggcg gcctcggacg caggcacgcc gagcctggac atgccggtat cgaccttcag
     2341 gtgcagcctg gccacgcggc cgcgcaactc ggcctgagcc gcgaccatcc tggcgttctc
     2401 gaccgagacc accgccacgg acaggccgcc ggcgaccgcc cggcccgctc cctcgggccc
     2461 cgtgtagccc atcacgagga tcggttcgac gacgccggcg gcccttaggg ccagcccctc
     2521 gtcgaccatg gccaccgcca gggacgtagc cccggcttca aggcaggccc tcgcgaccgg
     2581 cgccgcgccg tgcccgtagg cgtcggcctt gacgacggcg cacagccccg aatccccgcc
     2641 ggccagggcg acgaggcgct tgacgttcct tcgtatcgcc gagagatcca cctcggccca
     2701 tgcttgcctg tctatcatcg atgcgccctc ccaggcgcga cgtttgtacg cgaatgcggg
     2761 gttttcaacc ccgtgcaaca ctatcacgag cgtgaagcga cgacaatacg agcgggcccg
     2821 ccagcttcac caaaggccgc ctaggcgcta caatgcgagc cgcgctccgg gtaggacgcg
     2881 cgaggagcga cgcatgcgac ccctgatcgg cataacctcg ttcgacgacc agaagacccg
     2941 ctcgacctac gcggccatca acgccaacta cctctactcg atagacgccg cgggcgggat
     3001 cgccgtggtc ctaccaggcc cgagcgggct ccgtacggcc ggctccttcg ccgagccggg
     3061 cgacgacgag ctggccgagt ccgccgaggc ctacgccgcc gggctggacg ggctcctcct
     3121 gtcgggcggc ggcgacgtca gcccgcggct cctcggccgc tcgccgtccc gcggcgtggc
     3181 ccgcttcgac tcggaccgcg accgctggga tctggcgcta ttcgccgccg cccgccggcg
     3241 cggcctgccg gtactcggcg tctgccgggg ctgccaggtc gtcaacgtcg cgctcggggg
     3301 cggcctccac gaggacctgc cgaccgacct gcccggcgcg ggcggccatt ccttcgacgt
     3361 gccgatggac gagctggccc atgccgtgga catagagccg ggcagccgcc tcgcctcggc
     3421 cctcggccag acgcgcctcc ctgtcaacag cttccatcac caggccgcga gcggggccgc
     3481 cccgggcctc gtcgtgacgg ccagggccga cgacggcgtc atcgaggccc tcgagtcgcc
     3541 ggccggcgaa ccctacctcg tcgccgtgca gttccacccc gagggcctga ccaggcgcta
     3601 cccggccttt ctcggcatct tcaggggctt cgtcgagacc gcccgccgct aaggccccga
     3661 cgcgatccgc gcgcggtacg cgtcggcctc cttctcgttg gccagcacga agtgcccgcc
     3721 gccgacgtcg gtccacgccg gcttgtcggt cgagcagtcg tggcacttcg ggtcgtagac
     3781 catgaccacc ttcttccgct ccacgagcgg gtcgggctgg ggtatggccg aaatcagcgc
     3841 gcgcgtatac gggtggagcg ggttcctgaa tagctcctcg gtctccgcca gctcgacgat
     3901 ctcgcccttg ttgatcaccg cgata
//
LOCUS       DAOPHV010000205         3922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_31482_length_3922_cov_5.378071, whole genome shotgun sequence.
ACCESSION   DAOPHV010000205 DAOPHV010000000
VERSION     DAOPHV010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31482_length_3922_cov_5.378071"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PLQ29_06545"
     CDS             <1..904
                     /locus_tag="PLQ29_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86342.1"
                     /translation="WYLALAEDGSTGYVFSNQLRLWNAEEEPIPDLAAGSSISSESLS
                     LLFDNVWRPDYFDAMVAAGRLDLAAYQPRYGIFTDPLRNQIRVERPGFSRIYRYDSIE
                     ARDDGSYTILPGGASFRFTKAGRLVFVPAASDLSQEAAARLREDRGPDAELSYEFVRH
                     AQDVQSVVAAEERRRMSVLAGFVADGERFESEPYGALIITRSGRFTWAAYGALTPDVI
                     PEGAGEIGSISMDLYLSPELSVDWDGAFTLRFDGEKRPAVSFAYAMAGDELLLAYLPP
                     GSTRNAVVEAPDGLEPIAALKRYR"
     gene            909..2897
                     /locus_tag="PLQ29_06550"
     CDS             909..2897
                     /locus_tag="PLQ29_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPG86343.1"
                     /translation="MAFVQFTGVSLAFGARDILSDAALYLASGTKAALAGPNGAGKTT
                     LLRIVAGLLPPDSGDRAVQKGTRVSYLAQSGESYSGCTVYQEAEKAYGAVEALLAERD
                     SVGARLESSREGDDDLGSLLETYHHLDEAINGSGYWRRADRIREVLEGLGFKPEDAGR
                     QVGELSGGWQMRVALAKVLLENPDIMLLDEPTNYLDLEARDWLESYLGSFAGGFVVVS
                     HDRSFLDSTVNEVYELWNGRLTRYPGSYTAYERKRAQELETLFKAWEAQQEEIARLED
                     FIRRFRYQATKAAQVQSRIKQLDKVVPIVIPEGMKRVHFSFPPAPHSGRIALTLDGVR
                     KSYGPKRVVDELSLTVDAGSKIAFVGRNGAGKSTLMRLVAGVDGDYEGSIRLGTGITV
                     GYFSQDVADALDESLTVEEEASSACPTALLPGIRNLLGAFLFRGDDVEKRVSVLSGGE
                     RSRLALLKMLLHPANLLVLDEPTNHLDLDSKDVLLDALKRFDGTVLFVSHDRFFIEGL
                     ADRVLELSCGSSPRLFYGDYAYYLTKKAEERAGEVDEPARATRSGASFAPAEDGARER
                     NGRPLDAKASRELDKRRKAERNRLRKREDEILARMEAVGKEKADALAAMALPENYSDG
                     DRMRGLQARSQALDGEAAALSAEWEVVATELEGYADVD"
     gene            complement(2910..3638)
                     /locus_tag="PLQ29_06555"
     CDS             complement(2910..3638)
                     /locus_tag="PLQ29_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HPG86344.1"
                     /translation="MKRYILDAMVHLAVLIGGAALPAAILVILFRRADRARPEPVGLI
                     GRSVLYGFLATIPAIALELLVGIPAAYLPGLAGAAWSAFVTAALVEEGVKYFFLSRYL
                     LRNEAFDEVMDGIVYAACVSLGFAFAENLMYGVGGGWVLLFRAFTAVPMHAAATGIMG
                     YWLGLSKRESDSALAASCRRKGFIAAVVVHGFYDFFLFAGPPLAVGAILTLFVAVRAL
                     RRLVRRAKELDDAAASTRRALGLS"
     gene            3751..>3922
                     /locus_tag="PLQ29_06560"
     CDS             3751..>3922
                     /locus_tag="PLQ29_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86345.1"
                     /translation="MEQRIIDETAKSIRCLSMDAIQKANSGHPGLPLGCAELGAMLYG
                     EFMRHDPSDPAWL"
BASE COUNT          676 a         1350 c         1289 g          607 t
ORIGIN      
        1 ctggtacctg gcgctcgccg aggacggctc gaccggatac gtattctcca atcagctcag
       61 gctctggaac gccgaggagg agcccatacc cgacctggcg gccggctcgt ccatatcgag
      121 cgagtcgctg tcgctcctgt tcgacaatgt ctggcgcccc gattacttcg acgcgatggt
      181 cgctgccggg cgcctcgacc tggcggccta ccagccacgc tacggcatct tcaccgaccc
      241 cctgcgcaac cagatccgcg tcgagaggcc cggcttctcg agaatctacc gctacgactc
      301 tatcgaggcg cgcgatgacg ggtcgtacac catcctgccc ggaggcgcct ccttccgctt
      361 tacgaaagcc ggtcggctcg tcttcgtccc ggccgcgtcg gacctgtcgc aggaagcggc
      421 cgccaggctc agggaggacc gtgggccgga cgcggagcta tcctacgagt tcgtgcggca
      481 cgcccaagac gtccagtccg tcgtcgccgc cgaggagcgc cggcgcatgt cggtcctggc
      541 cggtttcgtc gccgatggcg agcgcttcga gagcgagccc tacggagcgc tgatcattac
      601 ccgctccggc cgcttcacct gggccgccta cggcgccctg acgcctgacg tcatccccga
      661 aggcgccggg gagatcggtt ccatatcgat ggacctgtac ctgtcaccgg aactatccgt
      721 cgactgggac ggcgctttca cgctgcgttt cgacggcgag aagcggccgg cggtttcctt
      781 cgcgtacgcg atggccggcg acgaactgct cctggcctac ctgcctccgg gctcgacgcg
      841 caacgccgtc gtagaggccc ctgacggcct cgagccgatc gcggccctca agcggtaccg
      901 ctagctccat ggcgttcgtc caattcaccg gcgtctcgct cgcgttcggg gccagggata
      961 tcctgtcgga cgccgcgctc tacctggcct ccggtaccaa ggcggctctc gccggtccga
     1021 acggcgccgg caagaccacc ctgctacgca tcgtcgcggg gctcctgccg ccggattcgg
     1081 gcgaccgggc cgtgcagaaa ggcacccgcg tctcgtactt ggcgcagtcc ggggagtcgt
     1141 actccggctg taccgtctac caggaagccg agaaggccta cggcgccgtc gaggccctgc
     1201 tggctgagcg cgactcagtc ggcgccaggc tcgaatcgtc gcgcgagggc gacgacgacc
     1261 tcggttctct gctcgagacc taccaccacc tcgacgaggc gatcaacggc tcgggctact
     1321 ggcgccgcgc cgaccgcatc cgcgaggtgc tcgagggcct cggcttcaag cccgaggacg
     1381 cgggccgaca ggtaggcgaa ctgtccggcg gatggcagat gcgcgtggcc ttggccaagg
     1441 tactacttga gaacccggat atcatgctgc tcgacgagcc gaccaactac ctggacctgg
     1501 aggcccgtga ctggctcgag tcgtacctgg gttccttcgc cggcggcttc gtcgtcgtgt
     1561 cccacgaccg ctccttcctc gactcgaccg tgaacgaagt ctacgagcta tggaacggca
     1621 ggctgacccg ttatcccggc tcctacacgg cctacgagcg taagcgggcc caggagctcg
     1681 agaccctgtt caaggcctgg gaggcccaac aggaggagat agccaggctc gaggacttca
     1741 tccgacgctt ccgttaccag gcgaccaagg ccgcccaggt ccagtccagg atcaagcaac
     1801 tggacaaggt cgtacccatc gtcatcccgg aaggcatgaa acgcgtccat ttcagcttcc
     1861 cgccggcccc ccattcgggg cgcatagcgc tgacgctcga cggtgtacgc aagtcctatg
     1921 ggccgaagcg cgtcgtcgac gagctgtcgc tgaccgtcga cgcgggctcg aagatagcct
     1981 tcgtcggccg caacggcgcg ggcaagtcga ccctgatgcg gctcgtcgcc ggggtcgacg
     2041 gcgactacga aggctcgata cggctcggaa cgggcattac ggtgggctat ttctcgcagg
     2101 acgtggccga tgcgctcgac gaatcgctga ccgtcgagga ggaggcgtcg tcggcctgcc
     2161 cgacggccct cctgccgggc atacgcaacc tgcttggcgc gttcctgttc cgtggcgacg
     2221 acgtggagaa gcgcgtctcg gtactgtccg gcggcgagcg ctcgcggctg gccctgctca
     2281 agatgctcct ccatccggcc aacctgctcg tgctcgacga gccgaccaac cacctcgacc
     2341 tcgattccaa ggacgtgctc ctcgacgcgc tcaagcgttt cgacggcacg gtgctgttcg
     2401 tgtcccacga ccgcttcttc atcgaaggcc tggccgatcg cgtgctcgag ctgtcctgtg
     2461 gcagctcgcc gcggctgttc tacggcgact acgcctatta cctgacaaag aaggccgagg
     2521 agcgggccgg cgaggtcgac gagccggcca gggccaccag gtccggcgcc tcgttcgctc
     2581 cggccgagga tggcgcacgc gagcggaacg ggcgtccgct cgacgccaag gcttcccggg
     2641 agctggataa gcggcgcaag gccgagcgca accgcctgcg caagcgcgag gacgagatac
     2701 tggcgcgtat ggaggccgtc gggaaggaga aggccgacgc gctggcagcg atggccctgc
     2761 cggagaatta ttcggacggg gatcgcatgc ggggcctcca agccaggtcg caggccctcg
     2821 acggcgaggc cgccgcgctg tccgccgaat gggaggtcgt ggctaccgag ctcgagggct
     2881 acgcggacgt ggattaagcc gcggcgggat caggaaaggc ccagggctcg ccgcgtcgag
     2941 gcggccgcgt cgtcgagttc cttggccctg cgtaccaagc gcctgagcgc ccgcacagcc
     3001 acgaacagcg tcaggatggc gccgacggcc agcggcgggc cggcgaacag gaagaagtca
     3061 tagaagccgt gcacgaccac cgccgcgata aaacccttgc gccggcagga cgcggccagc
     3121 gcgctatcgg actcgcgctt ggacaggccg agccagtagc ccatgatgcc cgtggcggcc
     3181 gcgtgcatcg gcaccgcggt gaacgcccgg aagagcaaca cccagccgcc cccgacgccg
     3241 tacatcaggt tttcggcgaa cgcgaaaccg aggctgacgc aggccgcgta gacgatgccg
     3301 tccatcacct cgtcgaacgc ctcgttgcgc aggaggtagc ggctcaggaa gaaatacttg
     3361 acgccttcct cgaccagggc ggccgtgacg aaggccgacc aggccgcccc cgccaagccc
     3421 ggcaggtagg ccgcgggtat gccgacgagc agctcgagcg ctatggccgg tatggtcgcc
     3481 agaaagccgt acagcacgct acggcctatc agcccgacag gctcgggcct ggcgcggtcg
     3541 gcgcggcgga acaggatgac gagtatcgcg gcaggtagcg cggccccgcc tatcagcacg
     3601 gcaagatgca ccatggcgtc cagtatatac cgtttcacga gatccatgga tagccgcgcc
     3661 ggcctgttgc cgcgctcgac cggccgtagt agtctagtat cctgagagct cgggcgatcg
     3721 cccgccacca taccccgcaa ggagcctata atggagcaac gcatcataga cgagaccgca
     3781 aaatcgatca ggtgcctatc gatggacgcc atacagaagg ccaattcagg ccacccgggc
     3841 ttgccgctcg gatgcgccga gctcggcgcg atgctgtacg gcgagttcat gagacacgac
     3901 ccgtccgacc cggcctggct cg
//
LOCUS       DAOPHV010000206         3915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_31562_length_3915_cov_4.786010, whole genome shotgun sequence.
ACCESSION   DAOPHV010000206 DAOPHV010000000
VERSION     DAOPHV010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31562_length_3915_cov_4.786010"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="PLQ29_06565"
     CDS             <1..1189
                     /locus_tag="PLQ29_06565"
                     /inference="COORDINATES: protein motif:HMM:NF017754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG86346.1"
                     /translation="TELRLANAVYLAPTGVSALSIGGQTIHSFFWIDPREPVHLETRL
                     DPERESRLYPLLAAVETLIVDEISMVRADLLDEMDRRMRQAKREPGLAFGGARVILFG
                     DPYQLPPVEPDEASRSGGAFAERYKSPFFFSSRVLRARARTMKRVEFTRVFRQCEDGF
                     IATLDRCRSGDVSDDDLRLLASRVLPEGKKPPADHLVLTATRAEAQRINVYRLAALPG
                     PSRVYAASADGRFAKDSGEDELPVPRELELKLGARVMLTASDEAGRWSNGALATVVGL
                     DEAGATLRDDAGEFRVGPHVWKRTRFALRDGGVVPVESDRYGQLPLQPGWALTIHRAQ
                     GLTLERVFVDLSRGAFAAGQAYVAISRVTRLDGLLLRARPRAKDFFVHERVSAFLEYL
                     KRQ"
     gene            1225..2385
                     /locus_tag="PLQ29_06570"
     CDS             1225..2385
                     /locus_tag="PLQ29_06570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPG86347.1"
                     /translation="MDFEGMNALTARATVHRRALHRIPETGLELPETVRYVRTALEAA
                     GIAPRDCGGGLLADIGDAGPLVAIRADMDALAVTEATGLPFSSAHPGAMHACGHDAHT
                     GALLACAERYAASPPEGFRVRLLFQPGEEGYFGARGMIADGCLDGVEAIVGSHVGHLS
                     DELSPGQAGFLPGPMMAASDLFSGAFVGSGGHGSAPHQALDPIPALAEFVLALQSFRS
                     RAPDQREPFVLSVCELSAGSAYNIIPCEAGFGGTVRSLSPTLRAMAKDGIARACAGVA
                     AAHGLRHRFDWVDGYPPLVNNAWASSLAMDATIRAIGAEHVRELTAPSMGGEDFAYYL
                     AKVPGCFWFLDTQPPGSAYPNHHPRFDIDERFLGRLAMVDMAIAASIANGLR"
     gene            2437..3399
                     /locus_tag="PLQ29_06575"
     CDS             2437..3399
                     /locus_tag="PLQ29_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HPG86348.1"
                     /translation="MQKHDGILIILNPIAGKGKAGERVPEIRRLMAAAGLDFELALTE
                     RVGHATELAASAGDRGFGLVVAAGGDGTVNEVVNGLMATKSAGKAAPVLAAFSVGRGN
                     DFAYGADLPATLEECVSVLARGERRAMDVGIVRGGDYPDGKYFANGLGVGFDTIVGLE
                     AAKLKRVHGFMAYVLGALKTFALFPDAPLVRLAWDGQRVEQECQQISIMNGKRMGGTF
                     FMAPNAENHDGSFDLCVAERLSRGEMVDLMIRYTKGTQAGHPKVKIYGGSRIVVEAPN
                     RGLIVHADGETICIDGERLDVECLAGALTIVCDPGRVARQKSMA"
     gene            3409..>3915
                     /locus_tag="PLQ29_06580"
     CDS             3409..>3915
                     /locus_tag="PLQ29_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPG86349.1"
                     /translation="MTTSSRIVTPAVKFILSRLCRIDSAEMRRVPKSGPLIVVMNHVN
                     FLEVPLLYSFLFPRDAIGIIKRETWDNPVLGALANSWQAIAIDREGADLSAMRKALEV
                     LERRGMLLIAPEGTRSGDGKLRKGHGGVVQLALRSGAPILPIAHHGGERFWPNIKSVR
                     RTRFVFRVG"
BASE COUNT          631 a         1293 c         1317 g          674 t
ORIGIN      
        1 gaccgagttg cggctggcca acgcagtcta tctagcgccg accggggtct ccgcgctttc
       61 gataggcggt cagactatcc atagtttctt ctggatcgac ccgcgagagc ccgtgcacct
      121 tgagaccagg ctcgacccgg aacgggaaag ccgcctctat cctctattgg ccgcggtcga
      181 gaccttgatc gtcgacgaga tctcgatggt ccgcgccgac ctcctcgacg agatggatcg
      241 acggatgcgc caggcgaaac gggagcccgg gctggcgttc ggcggagccc gcgtcatcct
      301 cttcggcgat ccataccaac tacctccggt cgaaccggac gaagcgagta ggtcgggcgg
      361 cgcgttcgcc gaacgctata aaagcccttt cttcttctcc tcgagagttc tgcgcgctcg
      421 agcgcggacg atgaagcgcg tcgagttcac ccgggtcttc aggcagtgcg aggatggctt
      481 catagcgacg ctggaccgtt gccgctcggg cgacgtgagc gacgatgacc tgcggctcct
      541 ggcctccagg gtccttcccg agggtaagaa gcccccggcc gaccatctcg tgctgacggc
      601 cacgagggcc gaggcccagc gtatcaacgt gtaccggctc gcggccttgc cgggcccgtc
      661 ccgcgtctac gccgcgagcg ctgacggccg cttcgccaag gactcgggcg aggacgagct
      721 gccggtgccg cgcgagctcg agctcaagct cggcgctagg gtcatgctca cggcgagtga
      781 tgaggccggt cgatggtcga acggcgcctt ggctaccgtc gtcgggctcg atgaggctgg
      841 cgccacgttg cgcgacgacg ccggtgagtt cagggtcggg ccccacgtct ggaaacggac
      901 tcggttcgcc ctccgtgacg gcggagtcgt tcctgtcgag tcggatcggt acgggcagct
      961 gccgctccag ccgggctggg ccttgacgat acatagggcc caggggctca cgctcgagcg
     1021 cgtcttcgtc gacctgtccc ggggcgcctt cgccgccggg caggcctacg tggccatctc
     1081 ccgcgtaacc aggctcgacg gcctcctgct ccgggcgcgg ccccgggcca aggacttctt
     1141 tgtccatgag agggtctcgg ccttcttgga gtacctcaag cggcagtaac ccgggcgcgg
     1201 tatggcgatg atatgggagg agctatggat ttcgaaggta tgaacgccct gacggctcgc
     1261 gccacggtcc ataggcgggc gctgcacagg atcccagaga cggggctcga gctacccgag
     1321 acggtacgat acgtcaggac ggcgctcgag gccgcgggca tcgccccgcg cgactgcgga
     1381 ggtggcctcc ttgccgatat cggcgacgca ggccccctcg tcgctatacg cgccgacatg
     1441 gacgccctgg ccgtcacgga agcaaccggc ctgccgttct cgagcgcgca tcccggcgcg
     1501 atgcacgcat gcggacacga cgcccatacg ggagcgctcc tggcctgcgc cgagcgctac
     1561 gcggcgtcgc ctccggaggg attcagggtg cggctcctgt tccagcccgg cgaggaaggg
     1621 tatttcggcg ctcgcggcat gatagctgac ggttgcctgg acggcgtcga agccatcgtc
     1681 ggctcccatg tcgggcacct gtccgacgag ttgtccccgg gccaggccgg tttcctgccc
     1741 ggcccgatga tggccgcgag cgatctgttc tccggcgcct tcgtcggttc aggcggacac
     1801 ggctcggcgc cgcaccaggc cctcgacccg ataccggccc tggcagaatt cgtgctggcc
     1861 ctgcagtcct tccgatccag ggcgcccgac cagcgcgagc cgttcgtgct cagcgtctgc
     1921 gagctgtctg ccggttccgc ctacaatatc atcccgtgcg aggccggctt cggcggtacc
     1981 gtccggagcc tttcgccgac gctcagggcc atggccaagg atggcatagc gcgcgcctgc
     2041 gccggcgtcg ccgcagccca cggcctacgg catcgattcg actgggtcga cggctatccc
     2101 ccgctcgtga acaacgcctg ggcgagctcc ctggcgatgg atgccacgat acgcgcgatc
     2161 ggcgccgagc acgtacgtga actgaccgcg ccgagcatgg gcggggagga tttcgcatac
     2221 tatctggcga aggtaccggg ctgtttctgg ttcctggaca cgcagccacc aggctcggcg
     2281 tatccaaacc accatccgcg cttcgacatc gacgagcgtt ttctcggtcg gctggccatg
     2341 gtcgatatgg ccatagccgc ctccatcgcg aacgggctac gctagcgcgt ttcccggcta
     2401 gcgtagcggc gcgttacggt ttatattctt gtcaccatgc aaaagcacga cggtatactg
     2461 attattctca acccgatcgc gggtaagggc aaggccgggg agcgagttcc cgagatacgg
     2521 cggcttatgg cggcggctgg gctcgatttc gagctggcct tgacggagcg ggtagggcac
     2581 gcgacggagc tcgccgcgtc ggcgggcgac cgcgggttcg gcctcgtggt ggctgccgga
     2641 ggcgatggta cggtcaacga ggtcgtcaac ggactaatgg ctacgaagtc cgcgggcaag
     2701 gccgccccgg tactggcggc gttcagcgtc ggccgcggca acgacttcgc ctacggcgcc
     2761 gacctaccgg ccacgctgga ggaatgcgtg tcagtgttgg cccggggcga gaggagggcg
     2821 atggacgtcg gcatcgtgcg cggcggcgat taccccgacg gcaagtattt cgccaacggg
     2881 ctcggcgtcg gcttcgacac catcgttggc ctcgaggctg ccaagctgaa gcgcgttcac
     2941 ggtttcatgg cctacgtgct cggcgccctg aagaccttcg cgctcttccc cgacgccccc
     3001 ctcgtcaggc tcgcctggga cggccagcgc gtcgaacagg aatgccagca gatatccata
     3061 atgaacggca agcgcatggg cggcaccttc ttcatggcgc cgaacgccga gaatcacgac
     3121 ggtagcttcg acctctgcgt cgcggagcgc ctgagccgcg gcgagatggt cgatctgatg
     3181 atccgctata ccaaggggac gcaggcggga catcccaagg tcaagatcta cggcggctcc
     3241 aggatagtcg tggaggcccc gaaccgcggc ctgatcgtcc acgccgacgg cgagactatc
     3301 tgcatcgacg gcgagcgtct cgacgtcgaa tgcctggccg gggcgctgac catcgtctgc
     3361 gaccccgggc gggtcgcccg gcagaagtcc atggcctgag gcccatacat gactacatcg
     3421 tctcgtatcg tcaccccggc agttaaattc atcctgtcga ggctatgcag gatcgactcg
     3481 gcggagatga ggcgcgtacc gaagtccggc ccgctcatag tcgtcatgaa ccacgtcaac
     3541 ttcctcgagg tgccgctcct atactcattc ctgttcccgc gcgacgcgat aggcatcatc
     3601 aagcgcgaga cctgggataa ccctgtcctg ggcgctctgg cgaactcgtg gcaggccatc
     3661 gcgatcgacc gagagggcgc cgatctgtcg gccatgcgca aggccctcga ggtgctggag
     3721 cgccgtggca tgctcctgat cgctccggag gggacgagga gcggcgacgg aaagctgcgc
     3781 aagggacatg gcggcgtggt ccagctggcc ctgcgcagcg gcgcgcctat cctgcccatc
     3841 gcgcatcacg gcggcgagcg cttctggccg aacatcaagt cggtacggcg gactcgtttc
     3901 gtcttcaggg tcggg
//
LOCUS       DAOPHV010000207         3905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_31682_length_3905_cov_3.344675, whole genome shotgun sequence.
ACCESSION   DAOPHV010000207 DAOPHV010000000
VERSION     DAOPHV010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31682_length_3905_cov_3.344675"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2212
                     /locus_tag="PLQ29_06585"
     CDS             <1..2212
                     /locus_tag="PLQ29_06585"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HPG86350.1"
                     /translation="LRGNAGPGRQDLLDAIRSKSVKVDEDVIDSLLAGIDVIKAMLGS
                     RSEGSPYGEDTSGLRTKLESFIPAKGAKPAAKKAPIPAPAPIPAVKAAPPAQPVASAA
                     ALTEYEMLEMREAAGTDRGVFLVSVDFDESNVMNTVSAIHAFAALREAGTVLKTEPDF
                     ESLYEDVFHPKVKYYVATKMSAEQLASAVRIPDVVTAVEVSTVSTDGAPIPKAPAAVS
                     APAPKPEATPPRVAVKAAAEPAEVPPVAASVQADREAPSDEEHEERSTDAKKAASQAG
                     SILRVDSKRIDDLLNLVSEAVINKATFNQVSTRFGDLQGGFQAAQGQFREGMKDLFDE
                     LPDYISSIQTGRTAKDIKKELLEKYGSLYSAFDSFEADFKGNVAKFRSTAQNLGRITS
                     ELQEGVMRIRMVPISQIFSRFPRLVRDLSKSLNKKVNLVIEGEDTELDKSVIEDLLDP
                     LMHSVRNALDHGIESAAERKAAGKPEEGTVMLRASNEGNMIIIEISDDGKGIDVEAVR
                     AKAIERGLIHPSKALTDIESFNLIFDPGFSTAAKVTNISGRGVGLDVVKRQIEKLNGS
                     VTVSSQRGKGSKFTIKLPLTLAIIQGLLVRVGRETYSIPITSVIESHRIKPSDIRMID
                     NYEVFNVRNDVVSLLRLNRLFRIQNAEQGDYCFVVIVGTSDKRVGLMVDSLIGEEDVV
                     IKPLRDQFTNSPGIAGASILGDGSVCLIIDVSQLLELGQKKELEERQRLELTIR"
     gene            2232..3575
                     /locus_tag="PLQ29_06590"
     CDS             2232..3575
                     /locus_tag="PLQ29_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HPG86351.1"
                     /translation="MSAQAADTLEAGKRPDEKKERADKIDFKMVTFSLAGKEYGIDIM
                     NVKEIANAGRFTYVPNAAPFVRGVYNLRGDIISIIDLRTMFHLPAERKDDDSLESLLI
                     LRVEDHAFGVIVDNIDKVVGVSSAGIQPPHPIFGDINVKYIKGIIENQGKLYIILNVE
                     KLFAPKDEVPEQEMSALRRGPAPVAAPATMVAPGPAGDSGPVGDVNAEFIRETLFAFR
                     RFGVSAVNDGWFKARYTEWKGMRKPTDLQLREQADADAYLETFYSRCTGELWSEDYAA
                     AVVEALPKVESRSINVWNPGCGKGYETYSLAVALRRAYPEARIKIWANDSDLLAISMA
                     PNMVFTPENVPEHYLDHMVKGKNGYTFNQSIRESIFFEFHDVMNSNPVPPVDFILCRD
                     TVSFLSAADQGKLFGDFCEKAKQGGAVILGTNERLPLDGWRRVGDGPAAVYVRTA"
     gene            3609..>3905
                     /locus_tag="PLQ29_06595"
     CDS             3609..>3905
                     /locus_tag="PLQ29_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HPG86352.1"
                     /translation="MRVEYINPFVESAFNVMKEVLQADVKRGELYLKSTSMPVMGVAA
                     IVGLAGDVEGRVLFDMDKPTALKIASAMNGEDIATMDDLAKATITELANMITAQA"
BASE COUNT          797 a         1270 c         1209 g          629 t
ORIGIN      
        1 cttgcgcggc aacgccgggc cggggcggca agacctgctc gacgccatcc gctcgaagtc
       61 ggtaaaggtc gacgaggacg tgatcgactc gctcctggcc ggtatcgacg tgatcaaggc
      121 catgctcggc tcgaggtcgg aaggctcgcc ctacggcgag gacacgagcg ggctacgtac
      181 gaagctcgag tcgttcatcc cggccaaggg cgccaagccg gccgccaaga aagctcccat
      241 tcccgctcca gctcccattc cggccgtcaa ggccgcgccg ccggcccagc ccgtcgcctc
      301 cgccgcggcg ctgaccgagt acgagatgct ggagatgcgc gaggctgccg ggaccgatcg
      361 cggcgtcttc ctcgtctcgg tcgacttcga cgagtccaac gtgatgaaca ccgtgagcgc
      421 cattcacgcg ttcgccgccc tgcgcgaggc cgggaccgtc ctcaagacgg agccagattt
      481 cgaatcgctc tatgaggacg tcttccatcc gaaggtcaag tactatgtag ccaccaagat
      541 gagcgccgag cagctggcct ccgccgtccg tatccccgac gtcgtgacgg ccgttgaggt
      601 gtcgacggtg tctaccgacg gagccccgat cccgaaggcg cccgccgccg tttcggcacc
      661 ggctcccaag cccgaggcaa cgccgccccg agtggccgtg aaagccgcgg cggagcccgc
      721 cgaggtccct ccggtcgcgg cgtcggtcca ggcggatcgc gaggctccaa gcgacgagga
      781 gcatgaggag cgttctaccg acgccaagaa ggccgcgagc caggccggca gcatcctccg
      841 cgtcgactcg aagcgcatcg acgacctgct caacctggta tccgaggccg tcatcaataa
      901 ggccaccttc aatcaggtca gcacccgctt cggagacctg cagggcggct tccaggccgc
      961 ccagggccaa ttccgcgaag ggatgaagga cctgttcgac gagctgccgg actatatctc
     1021 gtcgatccag acgggtcgca ccgccaagga catcaaaaag gaactgctcg agaagtacgg
     1081 ctctctgtac tcggccttcg attccttcga ggccgacttc aagggcaacg tcgccaagtt
     1141 caggtccacc gcgcagaacc tcggccgcat cacgagcgag ctacaggaag gcgtcatgcg
     1201 gatcaggatg gtgccgatca gccagatctt cagccgcttc ccccggctcg tgcgcgacct
     1261 atccaagagc ctcaacaaga aggtcaacct cgtcatcgag ggcgaggaca ccgagctcga
     1321 caagtcggtg atcgaggacc tcctcgaccc gctgatgcac tcggtgcgca acgccctcga
     1381 ccatggtatc gagagcgccg ccgagcgcaa ggccgccggt aagcccgagg aggggacggt
     1441 catgctgcga gcctccaacg agggcaacat gatcatcatc gagatctccg acgacggaaa
     1501 aggcatcgat gtcgaggccg tccgcgccaa ggcgatagag cgcggcctca tccacccgag
     1561 taaggcgctg acggacatcg agtcgttcaa cctgatcttc gaccccggct tctcgacggc
     1621 cgccaaggtc acgaacatct ccggacgcgg cgtcggcctc gacgtggtca aacgccagat
     1681 cgagaaactc aacggttcgg tcacggtgtc cagccagcgc ggcaagggta gcaagttcac
     1741 gatcaagctg cctttgaccc tcgcgatcat ccagggactg ctcgtgcgcg tcgggcgcga
     1801 gacctactcg ataccgatca cgagcgtcat cgagagccac cgtatcaagc cgtcggatat
     1861 acgcatgata gacaactacg aggtcttcaa cgtgcgcaac gacgtcgtct cgctcctgag
     1921 gctcaatagg ctcttccgca tacagaacgc ggagcagggc gactactgct tcgtcgtcat
     1981 cgtcggcacg agcgacaagc gggtgggcct gatggtcgac tcgctgatcg gcgaggagga
     2041 cgtcgtcatc aagccgctgc gcgaccagtt caccaattct ccaggcatcg ccggcgcgtc
     2101 gatactcggc gacggctcgg tatgcctgat catagacgtc agccagctgc tggagctagg
     2161 ccagaagaag gagctcgagg agcgacagcg cctcgagctg accatacgtt gaccacggga
     2221 gtaaggatcg catgagcgca caagccgccg atacgctcga ggccgggaag cggccggacg
     2281 agaagaagga acgggccgac aagatcgact tcaagatggt caccttctcg ctcgccggca
     2341 aggaatacgg catcgacatc atgaacgtca aggagatagc gaacgctggg cgcttcacct
     2401 acgtgccgaa cgcagccccc ttcgtccgcg gcgtctataa cctgcggggc gatatcatct
     2461 cgatcatcga cctacgtacg atgttccacc tgccggcgga gcgtaaggac gacgactcgc
     2521 tcgagagcct cctcatcctg cgtgtcgagg atcacgcctt cggcgtcatc gtcgacaaca
     2581 tcgacaaggt cgtcggcgtc tcgagcgccg gcatccagcc gcctcacccg atcttcgggg
     2641 atatcaacgt caagtacatc aagggcatca tcgagaacca gggcaagctg tacatcatcc
     2701 tgaacgtcga gaagctcttc gcgcctaagg acgaggtgcc cgagcaggag atgtcggccc
     2761 tgcgccgcgg tccggccccc gtcgcggcgc ctgcgaccat ggtagctccg ggtccggcgg
     2821 gtgactcggg ccccgtcggg gacgtcaacg cggagttcat ccgggagacc ctgttcgcgt
     2881 tcaggcgctt cggggtgtcg gcggtaaacg acggctggtt caaggcgcgc tacacagagt
     2941 ggaagggcat gcgcaagccg accgacctcc agctacgaga gcaggcggac gccgacgcgt
     3001 acctggagac cttctactcg cgctgtaccg gcgagctgtg gtcggaggat tacgccgccg
     3061 ccgtcgtcga ggcgctaccc aaggtggaat ccaggtccat caacgtctgg aaccccggct
     3121 gcggcaaggg ctatgagacc tattccctgg ccgtcgccct caggcgcgcc tatcctgagg
     3181 cccgtatcaa gatttgggcc aacgattccg acctgttggc catctcgatg gccccgaaca
     3241 tggtctttac cccggaaaac gtgccggagc actacctcga ccacatggtg aagggcaaga
     3301 atgggtatac cttcaaccag tcgatccggg agtccatatt cttcgagttc cacgacgtga
     3361 tgaactcgaa tcccgttccg ccagtggact tcatcctgtg ccgcgatacc gtatccttcc
     3421 tgtccgcggc cgaccagggt aagttattcg gcgatttctg cgagaaagcg aagcagggag
     3481 gcgccgtcat cctcggtacg aacgagcgcc tgcccctgga cggttggagg cgcgtcgggg
     3541 atggccccgc cgcggtgtac gtcaggacgg cctaggccgg cccagaaacg aacctacggg
     3601 agaatggcat gagagtagag tatatcaacc ccttcgtcga aagcgccttc aatgtcatga
     3661 aggaagtgct gcaggccgac gtcaagcgag gcgagctgta cctgaagtcg acctcgatgc
     3721 cggtcatggg cgtggcggcc atcgtcggcc tcgccgggga cgtcgaaggc cgtgttctgt
     3781 tcgacatgga caagccgacc gcgctcaaga tcgcctcggc gatgaacggc gaggacatcg
     3841 cgacgatgga cgacctggcc aaggcgacca tcaccgagct ggccaacatg atcaccgccc
     3901 aggcc
//
LOCUS       DAOPHV010000208         3901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_31730_length_3901_cov_4.803692, whole genome shotgun sequence.
ACCESSION   DAOPHV010000208 DAOPHV010000000
VERSION     DAOPHV010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31730_length_3901_cov_4.803692"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1194)
                     /locus_tag="PLQ29_06600"
     CDS             complement(73..1194)
                     /locus_tag="PLQ29_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HPG86353.1"
                     /translation="MNQIILGPLVVAGAAAALALVIALVDRVVNDYGEVTIDVNHGKR
                     LYKVRGGSPLLGTLASQGVFVPSACGGRGSCGACKVRVLSDVGPVLPTETPYLSPQEL
                     ANGVRLSCQVKLKKDIAVELPDELFSVKKFKATVERLDDVTYDIKDAVFRLDEPREIS
                     FTSGQYVQLVVPPYGTIKESVQRAYSMASRPGDSGRVELLIRLVPGGIATTYVHTALK
                     VGDRVELVGPFGDFRVRDTSAAMVCVAGGSGMAPFKSMFYDMRERDAFPDKDIWYFFG
                     ARSSRDMFYLDELRRLEAEWPRFHFVPALSEPSPEEGWNGETGLITDVLERYVKDRIP
                     RDKGLEGYLCGSPGMINACIAVMTRNGIAEGDIFYDKFA"
     gene            complement(1206..1799)
                     /locus_tag="PLQ29_06605"
     CDS             complement(1206..1799)
                     /locus_tag="PLQ29_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HPG86354.1"
                     /translation="MTPLINPVTLFFASIFTSNILLTNFLGTCSFISISKDWKSSFGL
                     GMAVTVVMTITAAVSWLVLEYVIAPLELEYLSFIVFIVVIAAVVQILEMVIDRASPAL
                     YMSLGIFLPLITVNCAILGAILFMQIRHYGFVQTVAFGAGSGLGWWLAILLLAAIRER
                     TEKNPVPAGLKGPGITMITIGFMAMAFIGFSGMIAVQ"
     gene            complement(1796..2422)
                     /locus_tag="PLQ29_06610"
     CDS             complement(1796..2422)
                     /locus_tag="PLQ29_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HPG86355.1"
                     /translation="MKAPKAVAILKDNLWANNPIFIQILGICSTLAVTNSLNNTLVMT
                     VGVALTTAFSSLTLSALKDLIPRKVRMIVQVVVISFYVILIDILLRAYLPEASRQLGP
                     YVGLIITNCIIMGRAEAFAQANPPLLSFWDGLTSGLGYMAVLMAIAAVRELLGFGTLF
                     GLRVMPEGFVPWTIMIMAPSAFFLVAMVMWWAKSVQARRQTAGKEAKK"
     gene            complement(2419..3030)
                     /locus_tag="PLQ29_06615"
     CDS             complement(2419..3030)
                     /locus_tag="PLQ29_06615"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPG86356.1"
                     /translation="MKKDSMLYVVLFTFAVCAVFTLALAIANEGTKDLVAANAEFATQ
                     SAVLGALGVPFDGKADAAARFAETVGAGEAPRSWLATVGGADYVAVEQSGPGLWGTIV
                     VILAATPDGERIRGVRVVSQNETPGLGGRIAEPWFLGQYEGERSPGGRVGMRAGAESS
                     GAADSDKENGRVDGVSGATRTSQSFAAIVDAALARVKESGGER"
     gene            complement(3027..>3901)
                     /locus_tag="PLQ29_06620"
     CDS             complement(3027..>3901)
                     /locus_tag="PLQ29_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPG86357.1"
                     /translation="PRVLATLAVSLGVGILVEYLFEKRKGGKVSEAVLVTGTLFALSM
                     PPAAPLWIVAIGIAFAVFMAKEVYGGFGRNVFNPAIAGRLFVYISFAGALGGSFFEPG
                     GFGAAAGSLFGRPDALSAATPLAVMRSGGSVPTLDLLLGTRAGSIGESSVVLIAVAAV
                     YLVATKTAQWRLIVSTLLGGAIVTGGLVVAGVARALPMDSLLAGSFLFVAVFMATDPV
                     SAPKKKPAQWAYGLLIGIAVAVIRNFSLFPEGTSFALLLGNTFASLFERIAADAAKRK
                     AAKAAQATGGEAKP"
BASE COUNT          686 a         1279 c         1293 g          643 t
ORIGIN      
        1 gccaggcttc atgcgctcgt acgctgctat ctcggccgtc gtgttcttca tatgccctcc
       61 agtgcggcta gtctaggcga atttatcgta gaagatgtcg ccctcggcga taccgttcct
      121 ggtcatcacc gcgatgcagg cgttgatcat gccggggctg ccgcagaggt agccctcgag
      181 gcccttgtcc cgcgggatcc tgtctttcac gtagcgctcg agtacgtcgg tgatcaggcc
      241 cgtctcgccg ttccagcctt cttccgggct cggctcggac agcgccggaa cgaagtggaa
      301 gcgcggccac tcggcctcga gccgccgcag ctcgtcgagg tagaacatgt cgcggctcga
      361 gcgcgcgccg aagaagtacc atatatcctt atccgggaag gcgtcgcgct cgcgcatgtc
      421 gtagaacata ctcttgaacg gggccatgcc cgagcctccg gcgacgcaga ccatcgcggc
      481 ggacgtgtcg cggaccctga agtcgccgaa tgggcctacc agctcgaccc ggtcgcctac
      541 cttgagcgcg gtatgtacgt aggtcgtggc gatgccgccg ggtaccaggc gtatcagtag
      601 ctcgacgcgc ccggagtctc ccggccgcga cgccatcgag taggcgcgct ggacgctctc
      661 cttgatggta ccgtagggcg ggacgacgag ctggacgtac tggccgctcg tgaacgatat
      721 ctccctgggc tcgtcgagcc tgaagacggc gtccttgatg tcgtaggtga cgtcgtcgag
      781 ccgctccacc gtcgccttga acttcttgac gctgaacagc tcgtcgggca gctcgacggc
      841 gatatccttc ttgagcttga cctgacagga taggcgaacg ccgttcgcca gctcctgcgg
      901 cgacaggtag ggcgtctcgg taggcagtac cggcccgaca tccgacagca cgcggacctt
      961 gcacgcgcca caggagccgc ggccgccgca ggccgacggt acgaagactc cctgcgacgc
     1021 gagcgtgccg aggagcggcg agccgcccct gaccttgtag aggcgcttgc cgtggttgac
     1081 gtctatggtc acctcgccgt agtcgttgac gacgcgatcc accaacgcta tgacgagggc
     1141 cagggcggcg gcggcgccgg cgacgacgag cggccccagt atgatctgat tcacgtccgc
     1201 ctcccttact gcaccgctat catgccggag aagccgatga aggccatcgc catgaagcct
     1261 atcgttatca tcgtgatgcc cgggcctttg aggcccgccg gtaccgggtt tttctcggtg
     1321 cgctcgcgta tcgccgccag aagcagtatc gccagccacc agccgaggcc ggagccggcc
     1381 ccgaaggcca cggtctggac gaagccgtag tgtcggatct gcatgaacag tatggcgccg
     1441 agtatcgcgc agttgaccgt gatcagcggc aggaagatgc ccaggctcat gtacagggcg
     1501 gggctcgcgc ggtcgatgac catctcgagt atctgcacca cggcggcgat gacgacgatg
     1561 aacacgatga acgacaggta ctcgagctcg agcggcgcta tcacgtactc gagcaccagc
     1621 cagctgacgg cggccgtgat ggtcatcacg accgtgacgg ccatgccgag cccgaaggag
     1681 ctcttccagt ccttggagat cgagatgaag gagcacgtgc ccaggaagtt cgtcaggagt
     1741 atgttgctcg tgaagatcga cgcgaagaac agcgtgaccg ggttgatgag cggcgtcatt
     1801 tcttagcctc cttgccggcg gtctgtcgcc tcgcctggac cgacttggcc caccacatga
     1861 ccatcgcgac caggaagaac gcgctcgggg ccatgatcat gatggtccag ggcacgaagc
     1921 cctccggcat gacgcgtagg ccgaacaggg tgccgaaacc caggagctcg cggaccgcgg
     1981 cgatggccat cagcacggcc atgtaaccga ggccgctcgt cagcccgtcc cagaacgaca
     2041 ggagcggagg gttggcctgg gcgaaggcct cggctcgacc catgatgatg caattcgtga
     2101 tgatcagccc gacatagggg ccgagctgcc tcgaggcttc cggcaggtag gcgcgtagca
     2161 ggatgtcgat caggatcacg tagaacgaga tgacgacgac ctgcacgatc attctgacct
     2221 tgcgcggtat caggtccttg agcgccgaca gcgtcaggct cgagaaggcg gtcgtcagcg
     2281 cgacgccgac ggtcatcacg agcgtgttgt tcaggctgtt ggtgaccgct agggtcgagc
     2341 agatgccgag tatctggatg aagatgggat tgttggccca gagattgtcc ttgagtatgg
     2401 cgacggcttt cggtgccttc atcgctcgcc tccgctttcc ttgacgcggg ccagggccgc
     2461 gtcgacgata gccgcgaacg actggctcgt gcgcgtggcg cccgagacgc cgtcaacccg
     2521 gccgttctcc ttgtccgagt cggccgctcc cgacgactcg gcgccggcgc gcatgccgac
     2581 gcgcccgccg ggcgagcgct ctccctcgta ctgcccgagg aaccagggct ccgcgatgcg
     2641 gccgccgagg ccgggcgtct cgttctgcga tacgacgcgg acgccgcgga tccgctcgcc
     2701 gtcgggcgtc gccgcgagta tcacgacgat ggtgccccag agcccggggc cggactgctc
     2761 gaccgctaca tagtcggctc cgcccaccgt ggccaaccac gaccgtggcg cctcgcccgc
     2821 gccgaccgtc tccgcgaagc gggcggccgc gtcggccttg ccgtcgaacg gtacgccgag
     2881 ggcaccgagc acggccgact gggtcgcgaa ctcggcgttg gcggcgacca ggtccttggt
     2941 gccctcgttg gctatcgcga gcgcgagcgt gaagaccgcg cagacggcga aggtgaacag
     3001 caccacgtag agcatcgagt cctttttcat ggtttcgcct ccccgccggt agcctgagcc
     3061 gccttggcgg ccttgcgttt cgcggcatcg gcggcgattc gctcgaacag gctcgcgaag
     3121 gtgttcccga ggagtagcgc gaagctggtg ccctcgggga acaggctgaa gttacgtata
     3181 accgcgaccg cgatgccgat caggaggccg taggcccact gggctggctt cttcttcggc
     3241 gccgagaccg ggtcggtggc catgaacacc gcgacgaaca ggaagctacc ggccaggagg
     3301 ctatccatcg gcaacgcccg cgcgacgccc gcgactacca ggcccccggt gacgatcgcg
     3361 ccgccgagta gcgtcgagac gatcagccgc cactgggccg tcttcgtggc gaccaggtag
     3421 accgcggcga cggcgatcag gacgacgctg gactcgccta tggaacccgc gcgcgtgccg
     3481 agcagcaagt cgagcgtcgg cacggaaccg cccgaacgca tgacggcgag cggggtggct
     3541 gcggagaggg catccgggcg gccgaacagg ctcccggccg ccgcgccgaa gccgccgggc
     3601 tcgaagaacg agccgccgag cgctccggcg aaggagatat acacgaaaag ccgtccggct
     3661 atggccgggt tgaacacgtt gcggccgaat ccgccgtaga cctccttggc catgaacacg
     3721 gcgaaggcta tacctatcgc gactatccat agcggggccg ccggcggcat cgacagcgcg
     3781 aacagcgtcc cggtcaccag taccgcctcg ctgactttgc cgcccttgcg cttttcgaac
     3841 aggtattcta ccagaatacc gacgccgagg ctcacggcca gggtcgccag gacccttggc
     3901 c
//
LOCUS       DAOPHV010000209         3890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_31867_length_3890_cov_3.712907, whole genome shotgun sequence.
ACCESSION   DAOPHV010000209 DAOPHV010000000
VERSION     DAOPHV010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31867_length_3890_cov_3.712907"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLQ29_06625"
     CDS             complement(<1..234)
                     /locus_tag="PLQ29_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPG86358.1"
                     /translation="MHDRQAALALWRRYNDDDSLLRHALSVEAAMRRFASARGEDPDY
                     WGLVGLLHDIDYQSHPDEHLKHAGAMLAEAGYPA"
     gene            309..1445
                     /locus_tag="PLQ29_06630"
     CDS             309..1445
                     /locus_tag="PLQ29_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPG86359.1"
                     /translation="MKPFAALALVVIASSLALPSGGAAADERLPAAAKAWLEERRVVV
                     FAGQEHYPPFEFIDERTGEYGGMAVELLRWIATEFGFTAVFRPMPFEEAQHAVLRGEA
                     DAMTGLFRSNERLLRFDFTEPAFNVPASIYARVGAEEPRSAAALRGRRVATQRGDYAI
                     EYLAMMATEVEWVYADDFGDAIGLLASGEADAMIGDEQVMDYQLQARRLGERIRKTSA
                     PLYVGVDCMAVAKGNVLLHAILNAGIARARKTGTLEMIYQKWTGQRLETQAAMARPTP
                     ATIAATVTAIAAVAAALLAKRRGGRLVAEATAALRGQADRLQAENEALAAANARLRVD
                     LEERSRLEVEKRRIDAEAAARRVEELTRCAIAKALDSAGGDGDS"
     gene            1581..2285
                     /locus_tag="PLQ29_06635"
     CDS             1581..2285
                     /locus_tag="PLQ29_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPG86360.1"
                     /translation="MLILSMLVLILTSIGAASTGPDILIVDSYVPYEFKELSGVRASD
                     FKSAMKGMSRADHIRDYYQNPDTRLATLSFFEQLTGSYHIARAILDESTARDVPPALA
                     FALAYEESGYDPQAFNRNPQSVDRGLFQLNSSSFPSLSITEFYDVAMNARIGMAHLAY
                     CLQQGGNEVAALAVYNAGLGRVSKGGTPRQTLDYIHRITGNRERLEALFEAQVVARHT
                     ESSSVAAALSSAADAN"
     gene            complement(2282..>2707)
                     /locus_tag="PLQ29_06640"
     CDS             complement(2282..>2707)
                     /locus_tag="PLQ29_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86361.1"
                     /translation="DGMAGADGMAGADGMAGADGMAGAVPSVEMLRRLSSLILRRCAD
                     QRRLLSVIVGYLEHSRKSGVDVEERIRRRTIRMRHIISGVVIAGQSKGELRAVPVGAV
                     SDFFYGMVEAAIFRLAVLGAERTDDLEAAMALFFEGLRA"
     assembly_gap    2708..2807
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2808..3044)
                     /locus_tag="PLQ29_06645"
     CDS             complement(<2808..3044)
                     /locus_tag="PLQ29_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPG86362.1"
                     /translation="MSATVEHEKRRKEILESALDVFVEEGYADTTFQKIADRCGITRT
                     ILYLYFKNKREIFMFSIKRFSEKLEAEIRAVEAGA"
     gene            3178..3843
                     /locus_tag="PLQ29_06650"
     CDS             3178..3843
                     /locus_tag="PLQ29_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HPG86363.1"
                     /translation="MVTAILFDLDNTLYPASADMERNTVRRMNEYAARLLGVDPEEAA
                     AVRRERMPGYGTTLEWLMAEHGFTDADDYFAYVHPDGEEDSVAFDPALGPFLDSIALP
                     KYVFTNAPMEHADRVLAKLGVADRFERVFDVRFCGLKGKPAASAVDKVLAAIGQPADS
                     TVFLDDVPRYVRGFIDRGGRGLLVDHFDAHRDSGLETIRTIYELPGRLAGGPYEKEDA
                     CRS"
BASE COUNT          689 a         1274 c         1215 g          612 t
ORIGIN      
        1 agccggatag ccggcctcgg cgagcatggc cccggcgtgc ttaaggtgct cgtcgggatg
       61 ggactggtag tcgatatcgt ggagcaggcc gacgaggccc cagtaatccg gatcctcccc
      121 gcgggccgag gcgaaacgcc gcatcgcggc ctccaccgat agcgcgtggc gcaggagcga
      181 gtcgtcgtcg ttgtaacggc gccagagggc gagcgcggct tgccggtcgt gcatcgtgcg
      241 tcctttccgc ggcccgggcg gggccgtagc gtaatggact atcctatcat atactcggcg
      301 gcgtgagaat gaaaccgttc gccgcgctgg ccctggtcgt catcgcctcg tcgctggccc
      361 tgccgtctgg cggggcggcc gccgacgagc ggctaccggc cgccgccaag gcctggctgg
      421 aggaacgccg ggtcgtcgtg ttcgccggac aggaacacta cccgccgttc gagttcatag
      481 acgagcgtac gggcgagtac ggtggcatgg cggtcgagct gctacgttgg atagcgaccg
      541 agttcggctt taccgcggta ttcaggccca tgcccttcga ggaagcccag cacgcggtgc
      601 tccgcgggga agccgacgcg atgacgggct tgttcaggag caacgagcgg ctcctgcgct
      661 tcgattttac cgaaccggcg ttcaacgtac cggcttccat ctatgcgcgc gtcggagccg
      721 aggagccgag gagcgccgcc gcgcttcgcg gccgcagggt ggccacccag cgcggagact
      781 acgcgataga atacctggcg atgatggcca ccgaggtcga atgggtctac gccgacgact
      841 tcggagacgc catcggcctc ctggcgtcgg gcgaggccga cgcgatgata ggcgacgagc
      901 aggtaatgga ctaccagctc caggcgcgca ggctgggcga gcggattcgc aagacctcgg
      961 ccccgctcta cgtgggcgtc gactgcatgg cggtggccaa aggcaatgtc ctcctccacg
     1021 ccatcctgaa cgccggcata gcgcgggcgc gaaagaccgg cacgctcgag atgatctacc
     1081 agaaatggac cggccagcgg ctcgaaaccc aggcagcgat ggcgaggccg acgccggcga
     1141 cgatagcggc gacggtcacg gccatcgcgg ccgtcgcggc ggcgctgcta gccaagcggc
     1201 gcggcggccg gctggtcgcc gaggcgaccg ccgcgctacg cggccaggcg gataggctac
     1261 aggccgagaa cgaggcgctg gcggcggcta acgcgaggct ccgcgtcgac ctcgaggaac
     1321 gttctcggtt ggaggtagaa aaacgccgga tagacgccga ggccgcggcc aggcgcgtgg
     1381 aggaactgac ccgttgcgcc atcgccaagg ccctggactc ggccggcgga gacggtgaca
     1441 gctaggctat tctgtctact acttgactcc cgcgcgaaaa acagtagtat atgacaggct
     1501 tactcattcg agtcgcaaac cccgcccagg agacttcccc cctaatggaa cccagaccaa
     1561 gccccagcgt ttcgatctcc atgctgatac tatccatgct cgtgctgata ctcacctcga
     1621 tcggagcggc ctcgacgggt cccgacatcc tgatcgtaga ctcctacgta ccgtatgagt
     1681 tcaaggagct gagcggcgtc agagcgtccg acttcaagtc cgcgatgaag ggcatgtccc
     1741 gagcggacca tatccgggac tactaccaga accccgacac caggctcgcc accctgtcgt
     1801 tcttcgaaca gctgaccggg agctaccata tcgcgcgcgc catcctcgac gagtcgacgg
     1861 ccagggacgt accgccggcg ctcgccttcg ccctcgccta cgaggaaagc ggctacgatc
     1921 cgcaggcgtt caatcgcaac ccgcaatcgg tcgaccgagg cctgttccag ctgaactcct
     1981 cgtcgttccc gtcgctcagc atcaccgagt tctacgacgt cgccatgaac gcgcgcatag
     2041 gcatggccca cctggcctat tgcctccagc aaggcggcaa cgaagtcgcc gccctggccg
     2101 tctataacgc cgggctcggg cgcgtcagca agggcggcac tccgaggcag acgctcgact
     2161 atatccacag gataacgggc aaccgcgaga ggctcgaagc gctcttcgag gctcaggtgg
     2221 tcgccaggca caccgagtcg tcgtccgttg ccgcggccct atcgtcggcc gccgacgcga
     2281 actaggcccg caggccttcg aagaacagcg ccatcgcggc ctccaggtcg tcggtgcgct
     2341 cggcgccgag cacggccagc ctgaagatgg ccgcctcgac catgccgtag aagaaatccg
     2401 acaccgcgcc gacgggcacg gcgcggagct ctcccttgct ctgcccggcg atcacgacgc
     2461 cggaaatgat atgcctcatg cgtatggtgc gccggcggat gcgctcttcg acgtcgaccc
     2521 cggacttcct cgaatgctcg agatagccga cgatcaccga cagaagccgc cgctgatcgg
     2581 cgcagcggcg gagaatgagc gaggatagcc tgcggagcat ctcgaccgac gggacggcgc
     2641 cggccatacc gtcggcgccg gccataccgt cggcgccggc cataccgtcg gcgccggcca
     2701 taccgtcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggc gccggcctcg
     2821 accgcccgga tctcggcctc gagcttctcc gagaagcgct tgatgctgaa catgaagatc
     2881 tcgcgcttgt tcttgaaata gagatacagt atggtacggg tgatcccgca acggtcggca
     2941 atcttctgga aggtcgtgtc ggcgtagccc tcttcgacga acacgtccaa cgcgctctcg
     3001 aggatctctt tcctgcgttt ctcgtgctct acggtcgcgg acacgggaac ctccaatctg
     3061 acgaaatacg gataccagtc taccttgacg gaataccatg tacgcgtcaa gtattttttt
     3121 attgccgacg agggcatcgc cttccatacg tcggcgtcag catggtatga agtatccatg
     3181 gttaccgcga tactcttcga tctcgacaac accctctacc cggcgagcgc cgacatggaa
     3241 cgcaacaccg tgcggcgcat gaacgagtac gccgccaggc tactcggcgt cgacccggag
     3301 gaggcggcgg ccgttcggcg cgagcgcatg cccggctacg gcacgacgct cgagtggctg
     3361 atggcggagc acggcttcac cgacgccgac gactacttcg cctacgtcca ccccgacggc
     3421 gaggaggact cggtcgcatt cgaccccgcc ctcggcccct tcctcgactc catagcctta
     3481 cccaagtacg tgttcaccaa cgcgccgatg gagcacgcgg accgcgtcct cgccaaactg
     3541 ggcgtggccg accgtttcga gcgggtcttc gacgttcgct tctgcgggct caagggcaag
     3601 ccggcggcct cggcggtcga caaggtgctc gccgccatcg gccagccggc cgatagcacc
     3661 gtattcctcg acgacgtgcc gcgatatgtc cgcggcttca tcgaccgcgg cggccgtggc
     3721 ctgctcgtcg accacttcga cgcgcatcgc gattcgggcc tcgagacgat acgcacgatc
     3781 tacgagctgc cgggccgcct ggccggcggg ccgtacgaga aggaggacgc atgccgttcc
     3841 tgagcctttt acccgacctg ataagtaagc gcaccgggcg cgtcgagctg
//
LOCUS       DAOPHV010000210         3887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_31910_length_3887_cov_4.156837, whole genome shotgun sequence.
ACCESSION   DAOPHV010000210 DAOPHV010000000
VERSION     DAOPHV010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31910_length_3887_cov_4.156837"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="PLQ29_06655"
     CDS             <1..1407
                     /locus_tag="PLQ29_06655"
                     /inference="COORDINATES: protein motif:HMM:NF027610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4932 domain-containing protein"
                     /protein_id="HPG86364.1"
                     /translation="YDAPPAFALSLEGGQAMRPPAGGWSDYLRGRAGGKRSLDRFAAA
                     LGELYADSGFDGFLAAHADDYARWLAEARAGFDAERVSAWLEAFYGKPEVPTVYHFVL
                     APAMFPGGGYGFSRRAGPAGAETLHIYQIVRAGGYQDGASSAGDAGEPGFPTGRSLAL
                     LGLHEFGHSFVNPALEAAWPRSGGAKGLEAVFAPVKAVMARQAYPEATTFYNELVIRA
                     VCVVAGRELGLLSEYEARAELSGEAGQGFYPIGRVVDLIAEYQAGRDRYPRFGDFAPA
                     LLERLAADAAAIVAEGSASGYRAVYDASLLPSEGFVEDFETVAERGLPPGFSVTVGAS
                     IGSGPASELGCDRREAASGAASFSLRADSETRSWLYLGKPVAVRPGTLTLRYRARGEG
                     IIKEAGQYGNSYVGFVVTLKDGSVVFPRRSHAGSFPWSAHEVEYEVVPSRIRSIEFIC
                     FLNESGGLWIDDIAVAYR"
     gene            complement(1429..3783)
                     /locus_tag="PLQ29_06660"
     CDS             complement(1429..3783)
                     /locus_tag="PLQ29_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPG86365.1"
                     /translation="MQNQRSAMHSLLESIEAESPSSLPDGLAMLEAHAGSAASQAGGQ
                     IPDAEASIFSQLMERILQGYALKLEDDGGESGASLEILLDGVSDSVLIMSPPGHILEA
                     NQSFYQTFGYARGELIGRPVFELLPDEYRGSFHTRLAEFSIRDGEPRAATSEDILAFR
                     GKRRDGSVVSMDCLLSGIQLRGEPAVIALIRDLSFDHSLFQQLKETREHYVALSETIT
                     EAIFRLDDNFDIIFVNSGVKNTFGWDRDEVIGKPFSVLFPPAVFAQHEIEFRKYFYVD
                     DQDRTALGLKRTFEFLGTTKHRGVAPMEMSFGNSKDCRGRTLTCVIRDITQRKTIERK
                     LRHLAYHDKLTGLGNRDLFNEDMAALLRNQDKDGSWRGAVLFLDLDGFKHVNDTLGHD
                     AGDELLIETARRLRVCLRDDDAAYRFGGDEFVVVLTAIKDASDGVLVSERILASVSSP
                     YILRSKNGTSGGSVSVGVSIGIAVIPEHGHTIEEATKSADIAMYCSKEGGKNRYTLFE
                     PSVSAKATERWRLEQEMKATLQRGGFNLLYQPIVGRDGRTLGMEALTRWKRDDERTVM
                     PGEFIPIAEETGMIVALGDWALRRACYDARRLHEHGCRDVYVSVNVSSRQFERPGYAE
                     ELEATVKGSGLAPSRLKLELTESAIMRNTADAIERIKDIKQRLPGISFMIDDFGTGYS
                     SLAYLSRLPVDALKIDISFVTNLAERQNEKVVNAIINLGHSLDIDIVAEGIETEEQWR
                     YFAEREVGMLQGYYFLRPSTLKDVYARIGREAKRRHPAGTPGAR"
BASE COUNT          559 a         1311 c         1306 g          711 t
ORIGIN      
        1 tacgacgcgc cgcccgcctt cgcactgtcg ctcgagggcg gccaggcgat gcggccgccg
       61 gccggcggct ggtccgacta cctgcgcgga cgcgccggcg gcaagcgctc gctcgaccgc
      121 ttcgccgcgg cgctcggcga gctgtacgcg gacagcggct tcgatggctt ccttgccgcc
      181 catgccgacg actacgcccg ctggctggcc gaggcgaggg ccggcttcga cgcggaacga
      241 gtctcggcct ggctcgaggc gttctacggg aagccggagg tcccgaccgt ctaccatttc
      301 gtcctcgcgc cggcgatgtt ccccggtggc ggctacggct tcagccgccg ggccgggccc
      361 gccggcgcag agacgctgca tatctatcag atcgtgcgcg ccggcggcta ccaggatggc
      421 gcgtcgtcgg ccggagacgc cggtgagccc ggcttcccga ccggccgtag cctggccctg
      481 ctcggcctcc acgagttcgg ccactcgttc gtgaacccgg ccctcgaggc ggcctggccg
      541 cgttctggcg gcgccaaggg gctcgaggcc gtcttcgctc cggtcaaggc cgtcatggcg
      601 aggcaggcct acccggaggc aaccacgttc tataacgagc tcgtgatccg ggctgtctgc
      661 gtcgtcgccg gtcgggagct gggcctgctg tccgagtacg aggcccgggc cgagctgtcc
      721 ggcgaggcgg gccagggctt ctatcccata ggccgcgtcg tcgacttgat cgccgagtac
      781 caggccgggc gcgaccgcta tccacgcttc ggcgacttcg cgcccgcgct gctcgagcgc
      841 ctcgcggcgg atgcggcggc catcgtcgcc gaaggctccg cgagcggtta tcgggccgtc
      901 tacgatgcct cgctcctgcc ttccgaaggc ttcgtcgagg atttcgagac ggtggccgaa
      961 cgagggctgc cgcccggctt cagcgtcacc gtgggcgcgt cgataggctc cgggccagcg
     1021 agcgagctcg gctgcgaccg gcgcgaggcg gcttcgggcg ccgcttcgtt ctcgttgagg
     1081 gcggactcgg agacccgctc ctggctctac ctcggcaagc ccgtggcggt caggccgggt
     1141 acgctgaccc tgcgctaccg ggcccgcggc gagggcatca tcaaggaggc ggggcagtac
     1201 ggcaacagct acgtcggctt cgtcgtgacc ctcaaggatg gctcggtcgt cttcccgagg
     1261 cgatcccatg cgggcagctt cccctggtcg gctcacgagg tcgagtacga ggtggtcccc
     1321 tcgcgcatac ggtcgatcga gttcatctgc ttcctgaacg agtccggcgg gctctggatc
     1381 gatgatatag cggtggccta tcgttagcgt gcgcgccggt cgtagctcct accgggcgcc
     1441 cggcgttccg gctggatggc gccgcttcgc ttcccggccg atacgcgcgt acacgtcctt
     1501 gagcgtcgat gggcgcagga agtagtagcc ctgcagcatg cccacctcgc gctcggcgaa
     1561 gtatcgccac tgctcctcgg tctctatgcc ctcggcgacg atatcgatat ccaggctgtg
     1621 cccgaggttg atgatggcgt tgaccacctt ctcgttctgt cgttctgcca ggttcgtcac
     1681 gaatgatata tcgatcttga gcgcgtcgac cggtaacctc gagaggtagg cgagcgacga
     1741 gtagccggtg ccgaaatcgt ctatcatgaa cgagatgccc ggtaacctct gcttgatgtc
     1801 cttgatgcgc tcgatcgcgt cggcggtgtt tcgcatgatc gccgactcgg tgagttccag
     1861 tttgaggcgc gacggtgcca ggcccgagcc cttgacggtg gcctcgagct cctcggcgta
     1921 gccagggcgc tcgaactgcc tgctcgacac gttgaccgac acgtacacgt cgcggcagcc
     1981 gtgctcatgc aggcggcgcg cgtcgtagca ggcgcgcctg agggcccagt cgccgagggc
     2041 cacgatcatg ccggtctcct cggctatcgg gatgaattcc ccgggcatca ccgtccgctc
     2101 gtcgtcgcgc ttccagcggg tcagcgcctc catgccgagc gtcctgccgt cccgcccgac
     2161 gatgggctgg tacagcaggt tgaagccccc gcgctgcagg gtcgccttca tctcctgctc
     2221 gaggcgccat cgctcggtcg ccttggcgct gacgctcggc tcgaacaggg tatagcggtt
     2281 cttgccgccc tccttcgagc agtacatcgc tatgtcggcg ctcttcgtcg cctcctctat
     2341 cgtgtggccg tgctcgggga tgacggcaat gcctatcgac actccgacgg agaccgagcc
     2401 gcccgaggtg ccgttcttcg aacgcagtat gtacgggctc gagacgctcg cgaggatgcg
     2461 ctccgagacc agcactccat ccgaggcgtc cttgatggcg gtcagcacga ccacgaattc
     2521 gtcgccgccg aagcggtagg cggcgtcgtc gtcgcgcagg cacacgcgca ggcgccttgc
     2581 cgtctcgatg agcagctcgt cgccggcgtc gtggccgagc gtgtcgttca cgtgcttgaa
     2641 gccgtccagg tcgaggaaca gcaccgcgcc ccgccacgag ccgtccttgt cctggttgcg
     2701 cagcagcgcg gccatgtcct cgttgaacag gtcgcggttc ccgaggccgg tcagcttgtc
     2761 gtggtaggcc aggtggcgca gcttccgctc tatcgtcttg cgctgcgtga tgtcgcggat
     2821 cacgcaggtc agcgtccggc cgcggcagtc cttggaattg ccgaacgaca tctccatcgg
     2881 cgcgacgccc cggtgcttgg tcgtgccgag gaactcgaag gtgcgcttga gcccaagggc
     2941 ggtacggtcc tggtcgtcga cgtagaagta cttgcggaat tcgatctcgt gctgggcgaa
     3001 cacggcgggc gggaatagca cgctgaacgg cttgcctatc acctcgtccc gatcccagcc
     3061 gaaggtgttc ttgacccccg agttgacgaa gatgatgtcg aagttgtcgt cgagcctgaa
     3121 gatggcctcg gtgatggtct ccgacagcgc cacgtagtgc tcgcgggtct ccttgagctg
     3181 ctggaacagc gagtggtcga acgacaggtc gcggatcagc gcgatgacgg ccggctcgcc
     3241 tcgcagctgg atgcccgaca gcaggcagtc catcgacacg accgagccgt cgcgccgctt
     3301 gccgcggaac gccaggatgt cctcggacgt cgccgccctc ggctcgccgt cgcgtatcga
     3361 gaactcggcc agcctcgtat ggaaggagcc gcggtactcg tcgggcagca gctcgaagac
     3421 cggcctgccg atcagctcgc ccctcgcgta gccgaaggtc tgatagaagg actggttcgc
     3481 ctccaggatg tggccgggcg gcgacatgat cagcaccgag tccgagacgc cgtcgagcag
     3541 gatctccagg ctggcgcccg actcgccgcc gtcgtcctcg agcttgagcg cgtagccctg
     3601 caggatgcgc tccatcagct gcgaaaaaat cgacgcctcg gcgtccggga tctgcccgcc
     3661 ggcctgggac gcggccgagc cggcgtgggc ctcgagcatg gctagcccgt cgggcaggga
     3721 gctcggactc tccgcctcta tggactcgag tagcgaatgc atcgccgatc gttgattctg
     3781 catcggagac tacgctcctt accgttttgc cattgttcgt gcgcgactgg ctctatcgat
     3841 accatattgt cggctcgtcc acgcccgcgt gcaagcgtat atgcgaa
//
LOCUS       DAOPHV010000211         3872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_32097_length_3872_cov_3.836521, whole genome shotgun sequence.
ACCESSION   DAOPHV010000211 DAOPHV010000000
VERSION     DAOPHV010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32097_length_3872_cov_3.836521"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PLQ29_06665"
     CDS             <1..649
                     /locus_tag="PLQ29_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG86366.1"
                     /translation="LVTLMLNYVALLLAEFLYYGPWRDPKGFGFPGTRLFPKEAWFPR
                     IAGRAHIGLYFAIAVALILWFVLNRTRWGFELKVIGNSPKAARYQGIAVERNIMLAIL
                     LSGAICGLAGVGEVAGISRRLQQGMALGYGFTAIIVAWMAQLNPLAVPFVALLMGALL
                     VGGDQVQMTMGLPSSIGTLMQGMILFPMLAGSLFTEYRIKRIGPQAVRESETGAA"
     gene            649..1599
                     /locus_tag="PLQ29_06670"
     CDS             649..1599
                     /locus_tag="PLQ29_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG86367.1"
                     /translation="MGLITSLLAITLRAGTSLVYATIGETYTERAGILNLGLEGIMLM
                     GAVSSFATAYYSGSLALAIVVALCVGATLSLIHAFLTVTMKANQVVSGLSITLFGTGF
                     ASFLGQRLGPASNGRYLIGLTGPRFAPIDVGPLKDVPILSAILNQDLFTYGIYILIPL
                     AWFYMYRTRNGLWLRAIGENPRTADAMGVNVNRSKYAYTIFGGMMTALGGAHLALAYT
                     PGWSENITGGRGWIVIALVIFSMWNPARAITGALIFGGINAVQFRLQASGTNIPAAFL
                     NMLPYLTTIAVLVAITWWEALSKRVGAPAALSVPYVREDK"
     gene            1605..3695
                     /locus_tag="PLQ29_06675"
     CDS             1605..3695
                     /locus_tag="PLQ29_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPG86368.1"
                     /translation="MDRRARARRAGPLAALAAFGSLAGPIAGAQALGGQSFGLDEDPR
                     RYEILRVAVEPGSPPLQFVDASGRPAGFLVDAVNAIGEEAGVAVALYAMAFPEAVAAL
                     AEGRIDAVLGVPYSAAALGEAGSLAVSEPLFASAVAVVSLADDGRYGEGSASLAGDVL
                     ALTRGSPAYDFLKAIRSVRFNETRTPADALELLSLGRADAFLEDRVVASYLLGRRDDA
                     ARFAVASSYWLPVEYGLAVRGGDDFLLYSLNQGVRAIKESGVYSAIYERWFDDSVAAA
                     RRRLRNAMMALVAVLAAFLLGGGAAFWWNRQLASNVRARTAELRDANDELGRLVGEAE
                     DKKEFIAQILESSPRGLVTCDAGGLVSVCNARARVIGLLDPDPVGRPALEYPFLAHFL
                     NPELTARVLGGESMPFSVSEWARPDGARLQIRHATYPQIDHEGRVVGLIFSFEDHTVE
                     QAMRERLAEREKGEALGRIVAGVAHEIRNPLTSIKAFVELLPRKMDDPRFLREMGSFV
                     PREVERVDKLIRDLIDFSRPRRPVRSTVDLGELAESTLALSSPSLDKRGVTTASSAER
                     GLYCSVDADQLRQCVMNFVLNAADSVEEARARGAGTAAAEDRGPDIELAAYASGGKAV
                     VELRDQGAGVAEADLERVFEPFYTTKRGGVGLGLPLSKQYVEENGGRLELRIGPGGGA
                     VVVMEFPLEGRERG"
     gene            3688..>3872
                     /locus_tag="PLQ29_06680"
     CDS             3688..>3872
                     /locus_tag="PLQ29_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPG86369.1"
                     /translation="MDRILIIDDEAAICSSLTFALEDAYSVASAVDADDGLAMASDEP
                     FDLVLLDLRIGKADGLEV"
BASE COUNT          551 a         1354 c         1329 g          638 t
ORIGIN      
        1 cctggtaacg ctgatgctca actacgtggc gctgctgctc gcggaattcc tgtactacgg
       61 cccgtggcgc gacccgaagg gcttcggctt cccgggcacg aggctcttcc ccaaggaggc
      121 ctggttcccg cgcatcgccg gccgcgccca cataggcctg tacttcgcga tagccgtggc
      181 gctgattctg tggttcgtgc tcaaccgcac gcgctggggc ttcgagctca aggtcatagg
      241 caacagcccc aaggccgcgc gctaccaggg catagccgtc gagcgcaaca tcatgctcgc
      301 gatcctgctg tccggggcca tctgcggcct ggccggcgtc ggcgaggtcg ccggcatctc
      361 gaggcgcctg cagcagggca tggcgctcgg ctacgggttc accgccatca tcgtcgcctg
      421 gatggcccag ctcaacccgc tggccgtgcc cttcgtcgcc ctcttgatgg gcgcgctgct
      481 cgtcggcggc gaccaggtgc agatgacgat gggcctcccc tcgtcgatag gcacgctgat
      541 gcagggcatg atcctgttcc cgatgctcgc cggatcgttg ttcaccgaat acaggatcaa
      601 gcggataggc ccccaggccg tccgcgagtc cgagacagga gccgcctgat gggcctcata
      661 acctccctgc tagccattac gctacgcgcc ggcacgagcc tggtctacgc gaccataggc
      721 gagacctaca ccgagcgcgc cggcatactc aacctcgggc tcgagggcat catgctgatg
      781 ggcgccgtct cgagcttcgc cacggcttac tactcgggca gcctcgcgct ggccatcgtc
      841 gtcgcgctgt gcgtcggagc gacgctgtcg ctgatccacg ccttcctgac ggtgacgatg
      901 aaggccaacc aggtggtctc cggcctgtcg atcacgctgt tcggcaccgg cttcgcgagc
      961 ttcctcggcc agcgcctcgg gccggcctcg aacggccgct acctgatagg cctgaccggc
     1021 ccgcgcttcg ccccgatcga cgtcgggccg ctcaaggacg tgccgatact cagcgcgata
     1081 ctcaatcagg acctgttcac ctacggcatc tatatcctga taccgctcgc ctggttctat
     1141 atgtacagga cgaggaacgg cctgtggctg cgggcgatcg gagagaaccc gaggaccgcg
     1201 gacgcgatgg gcgtgaacgt gaaccgctcg aagtacgcct acacgatctt cggcggcatg
     1261 atgacggcgc tcggaggcgc ccacctggcg ctcgcctata cgccgggctg gtccgagaac
     1321 atcaccggcg ggcgcggctg gatcgtgatc gccctcgtga tcttctcgat gtggaacccg
     1381 gcgcgcgcca tcaccggcgc gctgatcttc ggcggcatca acgccgtgca gttcaggctt
     1441 caggcttccg gcacgaacat cccggcggcc ttcctgaaca tgctgcccta cctgacgacc
     1501 atcgcggtgc tcgtcgcgat cacctggtgg gaggccctgt ccaagcgcgt cggggcgccg
     1561 gccgcgctca gcgtgcccta cgtcagggag gacaagtagc gacgatggat cggcgcgcga
     1621 gggcccgtcg ggccgggccc ttggcggcgc tcgcggcgtt cggttccctg gcggggccga
     1681 tcgcgggggc gcaggcgctg ggcggccagt ccttcggcct cgacgaggat ccgcgccgct
     1741 acgagatact cagggtggcg gtcgagcccg gctcgccgcc gctgcagttc gtcgatgcgt
     1801 ccggccgccc ggccggcttc ctggtcgacg cggtgaacgc gataggcgag gaggccggcg
     1861 tggccgtcgc gctctacgcg atggccttcc ccgaggccgt cgcggcgctc gctgaaggca
     1921 ggatcgacgc ggtgctcggg gtgccgtact cggcggccgc cctcggcgag gccgggagcc
     1981 tggccgtctc cgagccgctg ttcgcctcgg ccgtcgccgt cgtctcgctc gccgacgacg
     2041 gccgctacgg cgagggctcg gcgagcctgg ccggagacgt gctggcgctg acgaggggtt
     2101 cgccggccta cgacttcctc aaggccatac gctccgtgcg cttcaacgag acgaggaccc
     2161 cggccgacgc gctcgagctg ctctcgctcg gccgggccga cgccttcctc gaagaccgcg
     2221 tcgtcgcctc gtacctgctc gggcggcgcg acgacgccgc gcgcttcgcc gtggcctcgt
     2281 cgtactggct gccggtggag tacggccttg ccgtccgcgg cggcgacgac ttcctgctct
     2341 acagcttgaa ccagggcgtc cgcgcgatca aggagtccgg cgtctatagc gccatctacg
     2401 agcggtggtt cgacgactcg gtggcggccg cgcggcgccg cctgcggaac gcgatgatgg
     2461 ccctcgtcgc cgttctggcc gcctttctgc tcggcggagg cgcggcgttc tggtggaacc
     2521 ggcagctggc ctcgaacgtg cgcgcgcgga ccgccgagct gcgcgatgcc aacgacgagc
     2581 ttggccgtct cgtcggcgag gccgaggaca agaaggagtt catcgcgcag atcctggaat
     2641 ccagccctcg cggcctggtc acctgcgacg ccggcggcct cgtgtccgtc tgcaacgccc
     2701 gggcccgagt catcggcctg ctcgaccccg accccgtcgg ccgcccggcc ctcgagtatc
     2761 ccttcctggc gcatttcctg aatcccgagc tgacggcgcg ggtactcggc ggcgagtcca
     2821 tgccgttcag cgtctccgag tgggccaggc ccgacggcgc ccgcctgcag atacgccacg
     2881 ccacctatcc ccagatcgac cacgagggcc gcgtcgtcgg cctgatcttc tcgttcgagg
     2941 accatacggt cgaacaggcg atgcgcgagc ggctagcgga gcgcgagaag ggcgaggccc
     3001 tcggccgcat cgtcgccggc gtggcccacg agatccggaa cccgctcacc tcgatcaagg
     3061 ccttcgtcga gctgttgcct cgcaagatgg acgacccgcg cttcctgcgg gagatgggtt
     3121 ctttcgtgcc gcgcgaggtg gagcgggtgg acaagctcat ccgcgatctt atagatttct
     3181 cgcggccgag gcggccggtc aggtcgaccg tcgacctggg cgaactcgcc gagtcgacgc
     3241 tggccctgtc gtcgccctcc ctggacaaac gcggcgtcac gacggcctcg agcgccgagc
     3301 ggggcctcta ctgttccgtc gacgccgacc agctacgcca gtgcgtgatg aatttcgtgc
     3361 tgaacgcggc cgactcggtc gaggaggctc gggctcgcgg ggccggaacg gccgcggccg
     3421 aggaccgcgg cccggatata gagctggcgg cctacgcctc aggcggtaag gccgtcgtcg
     3481 agctgcgcga ccagggcgca ggcgtcgccg aggccgacct ggagcgcgtc ttcgagccgt
     3541 tctatacgac gaaacgcggc ggggtgggtc tcggcctgcc cctgtccaag cagtacgtcg
     3601 aggagaacgg cggacggctc gagctgcgga tagggccggg cggcggcgcc gtcgtagtca
     3661 tggagttccc cctggaagga cgagagcgtg gataggatac tgatcatcga cgacgaggcg
     3721 gccatctgct cgtcgctgac cttcgcgctc gaggacgcgt actcggtggc tagcgccgtc
     3781 gacgccgacg acggcctggc catggcgtcc gacgagccgt tcgacctcgt gctgctcgac
     3841 ctgcgcatcg gcaaggccga cggcctcgag gt
//
LOCUS       DAOPHV010000212         3862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_32222_length_3862_cov_4.285789, whole genome shotgun sequence.
ACCESSION   DAOPHV010000212 DAOPHV010000000
VERSION     DAOPHV010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32222_length_3862_cov_4.285789"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PLQ29_06685"
     CDS             <1..491
                     /locus_tag="PLQ29_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPG86370.1"
                     /translation="FVDLRSEPGEGAEFIVYLPVAQACGDACQEAVEPHRGTGAVLIV
                     DDDDIPRETAMSLLGALGYGAVAASSGDEARRLFEDRPAEWKAALLDLRLGDCSGDEL
                     AGRLRELRPDLPVVIASGLHEDSGGDAESGEAVIVGSAYALLHKPYTMGELARAMAEA
                     GA"
     gene            488..2365
                     /gene="recQ"
                     /locus_tag="PLQ29_06690"
     CDS             488..2365
                     /gene="recQ"
                     /locus_tag="PLQ29_06690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HPG86371.1"
                     /translation="MSVGASAESVLRDVFGYDSFRPLQLEIITAVLSGRDALAVLPTG
                     GGKSLCYQVPALILGGTTLVVSPLIALMRDQVAALRAAGVEAVFINSSLEAAERREIE
                     ARVRSGGVRLVYAAPESLAGDRLLSLLDDAPPRLIVVDEAHCVSEWGHDFRPEYRGLG
                     YLRERYPAAVWMATTATATERVRADIARCLGLRDPALFLGGFDRPNLSIRVERKLEVR
                     RRIVRFAQSRPDVSGIVYCGSRRRTEELAEALRAVGVKAAAYHAGLPAEERSWVQEAF
                     VRDDLLVVVATVAFGMGIDKPDVRYVVHADLPKSVENYYQEIGRAGRDGLPSECVLFY
                     GAGDVAAASRLFDELPDEQRRAALSRLEAMRDYAESERCRRALILEHFGEEGGADCGR
                     CDNCLAGPRASVDLSIPAQKLLSCVKRTGERFGAAHIVDVLLGERTEKVERFSHDGLT
                     TFGIGTELGRSAWMALARRLQSTGHLVREPERQTLSLGRPAYELFRDRGPYLVPVDLI
                     GGSTNGGSATERALDAKRSKRRRRDDSEPRQAIARSSDDGDLASEELFMQLRSLRKRL
                     ADESGVPPYVVFPDRTLKEMAALKPTTEASLATVYGVGRAKLERYGAAFLELLGGSRR
                     D"
     gene            complement(2362..3144)
                     /locus_tag="PLQ29_06695"
     CDS             complement(2362..3144)
                     /locus_tag="PLQ29_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86372.1"
                     /translation="MIRVKTLPLAIAVVAVMFGGVALSKAVGYWNTTSTKEPAKFKNG
                     ELAGLPNPADIRGSYTWLDLEKAFGVPAAESARAFSTADRALDPAERVSVLEELYLPI
                     LPPGLEIGTGAVRLFVSLYTGLPLEAEEGSVLPDGALELLSQRPGMDDEALGRYVIPS
                     NAPAAPAPAAPVPAAASPAASQSGTSQAGTGSGQATARSIVGKTTFGDLYDWGLTEDQ
                     VAAAIGYAPGPRSQSVRDSATANGKEFSELKIALQALVDAKP"
     gene            complement(3141..>3862)
                     /locus_tag="PLQ29_06700"
     CDS             complement(3141..>3862)
                     /locus_tag="PLQ29_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPG86373.1"
                     /translation="AMAMTPRATGDSCTFLTRVHESSVALGVIAIALAALFGPVVCGW
                     ICPLGSLQEWLGALGRTLFKKRYNAFIPGRVDKPLRYLRYLSLVWVSYMTIVSGKLVF
                     QDYDPYFALFNFWTGEVAIAGLVILDLTLVLSLFVERPFCKYACPFGAFLGLFNLFRV
                     FSVKRVASTCISCKACDRACPMSITVSTSGTVRDHQCISCLKCTSEDACPVEATVVLA
                     AGKPEIAAEPLQATGSEEVSR"
BASE COUNT          605 a         1283 c         1378 g          596 t
ORIGIN      
        1 gcttcgtcga cctgcgctcc gagccggggg agggggcgga gttcatcgtc tacctgccgg
       61 ttgcccaagc ctgcggcgac gcctgccagg aagcggtgga accccaccgc gggaccggag
      121 cggtgctcat cgtcgacgac gacgatatac cgcgggagac ggccatgtcc ctgctcggcg
      181 cgctcggcta cggggccgtc gccgcctctt cgggcgacga ggcgcggcgg ctgttcgagg
      241 atcgtcccgc ggagtggaag gccgcgctgc tcgaccttcg cctaggagac tgctcgggtg
      301 acgagctggc cggccggttg cgggagctca gacccgacct gcccgtcgtg atagcgagcg
      361 gcctgcacga ggatagcggc ggcgatgcgg aatccggcga ggccgtcatc gtcggctcag
      421 cctacgccct gctccataag ccgtatacca tgggagagct ggcgcgcgcc atggcggagg
      481 ccggggcttg agcgtcgggg ctagcgccga gtcggtgctc agggacgtgt tcggctacga
      541 ctcgttcagg cccttgcagc tcgagatcat caccgcggtc ctgtccgggc gagacgcgct
      601 ggccgttctg cctaccggcg gcggcaagtc gctgtgctac caggtaccgg cgctgatcct
      661 gggcgggacg acgctggtcg tctcgccgct gatcgcgctg atgcgcgacc aggtggccgc
      721 gctgcgcgcc gccggcgtcg aggcggtctt cattaacagc tcgctcgagg ccgctgagcg
      781 gcgcgagatc gaggccaggg tgcgctcggg gggcgtacgc ctcgtgtacg ccgcgccgga
      841 gtcgctggcc ggcgatcggc tcctgtcgct gctcgacgac gcgccgccca ggctgatcgt
      901 cgtcgacgag gcccactgcg tctccgagtg gggccacgat ttccggccgg agtaccgtgg
      961 cctcggctac ctacgcgagc gctacccggc cgccgtctgg atggcgacca cggccacggc
     1021 gaccgagcgc gtccgcgccg acattgcgcg ttgcctcggg ctgcgcgacc cggcgctgtt
     1081 cctcggcggc ttcgaccggc cgaacctgtc catccgggtc gagcggaagc tagaagtacg
     1141 gcgcaggatc gtgcgcttcg cgcagtccag gcccgacgtc tcgggcatcg tctattgcgg
     1201 ctccaggcgc aggaccgagg agctggccga ggcccttcgg gcggtcggcg tcaaggcggc
     1261 cgcctaccat gcggggctgc cggcggaaga gcgctcctgg gtacaggagg ccttcgtgcg
     1321 ggacgacctc ctcgtcgtcg tggccaccgt cgccttcggc atgggcatcg ataagcccga
     1381 cgtacgctac gtcgtccacg ccgacctgcc gaagagcgtc gagaactact atcaggaaat
     1441 aggacgggcc ggacgcgatg gcctgccgtc cgagtgcgtg ctgttctacg gagccggcga
     1501 cgtggcggcg gcctccaggc tattcgacga gctccccgac gagcagcgcc gggctgcgct
     1561 ctcgaggctc gaggcgatgc gcgactacgc cgagagcgag cgctgccggc gggcgctgat
     1621 cctcgagcat ttcggcgagg agggcggcgc cgactgcggc cgttgcgaca actgcctcgc
     1681 cggcccacgc gctagcgtcg acctgtcgat cccggcccag aagctgctgt cgtgcgtcaa
     1741 gcggaccggc gagcgcttcg gcgcggccca tatcgtcgac gtgctcctgg gggagcggac
     1801 cgagaaggtc gagcgcttca gccacgacgg cctgaccacc ttcggcatcg gcacggagct
     1861 tggccggagc gcgtggatgg cgctggcgag gcggctccag tcgaccggcc acctcgtgcg
     1921 ggagccggag cgccagactc tgtcgctcgg gcgtccggcc tacgaactgt ttagagaccg
     1981 gggaccctac ctcgtaccgg tcgacctcat cggcggctcg acgaacggcg gctccgcgac
     2041 cgagcgcgcc ctggacgcca aacgctcgaa gcgacgcagg cgcgacgaca gcgagccgcg
     2101 ccaggccatc gctcgctcca gcgacgacgg ggatctggcc tcagaggagc tgttcatgca
     2161 gctcaggtcg ctcaggaagc gcctggccga cgaatccggc gtgccgccct acgtcgtgtt
     2221 ccccgaccgg acgctcaagg agatggccgc gctcaagccg accaccgagg cctcgctcgc
     2281 gaccgtctac ggagtcggcc gcgccaagct cgaacgctac ggcgcggcct tcctcgagct
     2341 gcttggcggc tcccgccgcg actagggctt ggcgtcgacc agggcctgga gcgcgatctt
     2401 gagctcggag aattccttgc cgttggccgt ggcgctgtcg cgtacgctct gcgacctcgg
     2461 gccgggcgca tagccaatag ccgcggcgac ctggtcctcg gtgaggcccc aatcgtacag
     2521 gtcgccgaag gtggtcttgc cgacgatgga gcgtgcggtc gcctggccgc ttccggtacc
     2581 ggcctgagac gtgccggact ggctcgcggc ggggcttgcc gccgctggaa cgggagccgc
     2641 cggagcggga gccgccggag cgttggacgg aatgacataa cggccgagcg cctcgtcatc
     2701 catccccggc cgctgcgata gtaactcgag ggcgccgtct ggcagcaccg agccttcctc
     2761 cgcctcgagc ggcaggccgg tatagagcga cacgaacagc cgtacggctc ccgtgccgat
     2821 ctcgaggcct ggaggcagta tcggcaggta tagctcctcg agcacgctga ccctctcggc
     2881 cgggtcgagg gcgcggtcgg cggtggagaa agcccgagcc gactcggcgg cgggtacgcc
     2941 aaaggccttc tcgagatcca gccaggtata ggagcctcgt atatcggcgg ggttcggcag
     3001 gccagcgagc tcgccattct tgaacttggc gggctccttg gtcgatgtcg tattccaata
     3061 gccgacggcc ttcgatagcg cgacgccgcc gaacatgacg gcgacgaccg ctatggctaa
     3121 cggaagcgtc ttgacgcgta tcatcgcgat acctcctcgg agccggtcgc ctgaagaggc
     3181 tcggctgcga tctcaggctt cccggcggcg agcacgacgg tcgcctctac ggggcaggcg
     3241 tcctcgctcg tgcacttgag gcagctgatg cactgatggt cgcggacggt accgctcgtc
     3301 gataccgtta tgctcatcgg gcaggcgcgg tcgcaggctt tgcacgagat gcaggtcgac
     3361 gcgacgcgct tgacgctaaa cactctgaac aggttgaaca ggcccaggaa cgcgccgaag
     3421 ggacaggcgt acttgcagaa aggccgttcg acgaacagcg agaggacgag agtcaggtcg
     3481 aggatgacga ggccggctat ggcgacctcg ccggtccaga agttgaacag ggcgaagtac
     3541 gggtcgtagt cctggaagac cagcttgccg ctcacgatcg tcatgtagga gacccagacc
     3601 agggagaggt agcgcaggta gcgaagcggc ttgtcgacgc gcccgggtat gaaggcgttg
     3661 taccgtttct tgaacagcgt gcggcccagg gcgccgagcc attcctgcag gctgccgagg
     3721 gggcatatcc agccgcatac caccgggccg aatagcgcgg cgagcgctat ggctatgacg
     3781 ccgagggcta ccgacgactc gtgcaccctc gtcaagaaag tgcacgagtc gccggtagcc
     3841 ctcggcgtca tagccatagc gc
//
LOCUS       DAOPHV010000213         3848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_32385_length_3848_cov_4.261534, whole genome shotgun sequence.
ACCESSION   DAOPHV010000213 DAOPHV010000000
VERSION     DAOPHV010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32385_length_3848_cov_4.261534"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLQ29_06705"
     CDS             complement(<1..205)
                     /locus_tag="PLQ29_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86374.1"
                     /translation="MCDCFYLPTSRGAFFGKNSDRHATEPQALRLVPRRRPSPSVSFG
                     GKSFELPDAGHALALSCPCWMPGA"
     gene            503..1777
                     /locus_tag="PLQ29_06710"
     CDS             503..1777
                     /locus_tag="PLQ29_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPG86375.1"
                     /translation="MKYLVLAIAVVMYGLVIAFPHKKAWFTLGAALAVAALGVVSPAV
                     IFGELINWNVLLIYVGSLVIADLFIYSRVPSRLADRIVARAPNLGWGVVFILIMTGLL
                     SAFVENVATVLVMAPIALALSDKMKVDARYFMIGLAVMSNLQGTATLVGDPPSMVFAG
                     FAGYSFNDFFFKDGKPGVFFAVQVGMLVGAAFFYYYYRGMKNEKVDLPDERVKSWFPT
                     LLLAALIVGLAVISFLFEGFSILSGGFVFALGLVGLAWYVGVMKESRAQAWRLVKSLD
                     WETILFLIGIFVVVGAVSETGLLEDLSSLLASLVGDSVLTGFVLIILMSVLLSGFIDN
                     VPYIIVMLPVAAKLAAGLGLRPELYMFGLLIGSCLGGNLTPFGASANVVAVSMLKKRG
                     TVVRFSEWIKVAGPFTVLTTLASSLFVWLVWS"
     gene            complement(1924..2937)
                     /locus_tag="PLQ29_06715"
     CDS             complement(1924..2937)
                     /locus_tag="PLQ29_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPG86376.1"
                     /translation="MNISDYRGVMVFAEQRRGIVQKIAYELLGVGRRIADDLEEPLLA
                     LLVGHGLPDSAAAELIAQGADTVVVVDDPALDVYMTEPYAKAMTAAIERYKPGIVFTG
                     ATTIGRDLAPRVSARVRTGLTADCTGLEIEDGTRNLLMTRPAFGGNIMATIVCPEHRP
                     QMSTVRPGVMRRLERDDSRKGAVERLVVTLEEKDRNVEILEVVVEKAAKKSIEEANVL
                     ISGGRGLGNLDNFNKLQGVADELGGLVSASRAVVDAGWVGQDRQVGQTGKTVRPDVYF
                     ACGISGMIQHVAGMEDSDFIVAINKNSSAPIFEYADVGIVADINKVVPALVEELKAAK
                     AAR"
     gene            complement(2963..3742)
                     /locus_tag="PLQ29_06720"
     CDS             complement(2963..3742)
                     /locus_tag="PLQ29_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPG86377.1"
                     /translation="MDIVVCMKQVPDTTEVKIDPVTGTLIREGIPSIINPDDKGGLEA
                     ALALKDELGAKVTVLSMGPPQADLALREALAMGADEAVLLSDRAFGGADTWATSTTIA
                     AALRKLGFDLIICGRQAIDGDTAQVGPQIAEHLGLPHVSYVSELEATEGSVVVKRVFE
                     DGYQRIKVKLPCLLTALKDMNRPRYMSVSGIFDAYRREVKVWGLADIEVDQDNIGLKG
                     SPTRVKKSFTKGVKSAGKVYEVDAKEGARIIVEALKEKYVI"
     gene            complement(3771..>3848)
                     /locus_tag="PLQ29_06725"
     CDS             complement(3771..>3848)
                     /locus_tag="PLQ29_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPG86378.1"
                     /translation="DAKITEIYEGTSEVQRMVISANMLK"
BASE COUNT          621 a         1285 c         1186 g          756 t
ORIGIN      
        1 cggcgccggg catccaacac gggcaggaca gagcgagcgc gtgaccggcg tccggaagct
       61 cgaacgactt gccgccgaaa gagacggacg gcgacggtct acgcctaggc acgagccgca
      121 gggcctgagg ctcggtggca tgccggtcgg aattcttacc gaagaacgct ccccgcgagg
      181 tgggaaggta gaaacagtca cacatggttc cctccgctcg catagtacta taatcgcgga
      241 acgccgccat gacaaatccg tagcggccgg cgcggcttga cctccggctc cgatatacgt
      301 atcctcacgg gcgtatcgag aggggagtag ttgcatcgtc cgcatcaaca accggcgccc
      361 agaaacgggt cagcctggtc ggacggccgc cgtagcctta ccgaggcgcc gtcggaaaca
      421 agacctttcg catgcgcccg tttacggacc catgcgaaag gtcttttttt agcgcgacag
      481 cccgccgcga aggagtaatc gcatgaagta tctggtactg gctatcgccg tcgtcatgta
      541 cggcctcgtg atagccttcc cgcacaagaa ggcctggttc acgctcggcg cggccctagc
      601 ggtcgccgcg ctcggcgtcg tatcgcccgc cgtcatcttc ggcgagctga tcaactggaa
      661 tgtcctgctc atctacgtag gcagcctcgt catcgccgac ctcttcatct actcgcgcgt
      721 gccgtctcgg ctcgctgaca ggatagtagc cagggcgccg aacctgggct ggggcgtcgt
      781 gttcatcctg atcatgacgg gcctcctgtc cgccttcgtc gagaacgtcg ccaccgtgct
      841 cgtgatggcg ccaatagcgc tggcgctcag cgacaagatg aaagtggacg cgcgatactt
      901 catgatagga ctcgcggtca tgtcgaacct ccagggcacc gcgacgctgg taggcgaccc
      961 gccatcgatg gtattcgccg gcttcgccgg atactcgttc aacgacttct tcttcaagga
     1021 cggcaagccc ggcgtgttct tcgcggtaca ggtaggcatg ctcgtaggag cggcgttctt
     1081 ctattactac taccgcggca tgaagaacga gaaggtcgat ctgccggacg agcgcgtcaa
     1141 gtcctggttt ccgactcttc tgctcgcggc gctcatcgtc ggccttgccg tcatatcgtt
     1201 cctgttcgag ggcttctcca tactgtcggg cggcttcgtc ttcgcactcg gcctcgtcgg
     1261 actcgcgtgg tacgtcggcg tgatgaagga gagccgcgcc caggcttggc gcctcgtcaa
     1321 gtcgctcgac tgggagacga tactattcct gatcggcatc ttcgtcgtcg tcggggccgt
     1381 gtcggagacc ggcctgctcg aggacctgtc gtccctacta gccagcctcg tcggcgacag
     1441 cgtgctcacc ggcttcgtgc tcatcatact catgtcggtg ctgctctcag gcttcatcga
     1501 caacgtaccc tatatcatcg tgatgctgcc cgtcgcggcc aaactcgccg ccgggctcgg
     1561 cctgcgcccg gaactgtaca tgttcggcct gctcataggc tcgtgcctcg gcggcaacct
     1621 gacgccgttc ggcgcttcgg ctaacgtcgt cgccgtgagc atgctcaaga aacgcggcac
     1681 cgtggtacgc ttcagcgagt ggatcaaggt agccggaccg ttcaccgtcc tgactaccct
     1741 ggcttcgagc ctgttcgtct ggctcgtctg gagctaggcg ccgtccgcca gcggaacgcg
     1801 aagcgcaagg cgacggacct gtccaggcct gcttaaatag cgcgcggccg gatcgatcga
     1861 tccatacgaa aaaggccgcc ccggcggggc ggcccaatct agccgatagg tcgctctagc
     1921 ctatcaccgc gccgccttgg cggccttgag ctcctcgacg agggccggca ccaccttgtt
     1981 gatgtcggcg acgatgccga catcggcgta ctcgaagatc ggcgccgacg agttcttatt
     2041 gatcgcgacg atgaagtccg agtcctccat gccggcgacg tgctggatca tgccggagat
     2101 gccgcaggcg aagtacacgt ccggccgaac cgtcttgccg gtctggccga cctgccggtc
     2161 ctgccccacc cagcccgcgt cgaccaccgc gcgcgacgcc gacacgaggc cgccgagctc
     2221 gtcggcgacg ccttgcagct tgttgaagtt gtccaggttc ccgaggccgc ggccgccgga
     2281 gatgagcacg ttcgcttcct cgatgctctt cttggccgcc ttctcgacga ccacctcgag
     2341 gatctccacg ttccggtcct tctcctcgag cgtgaccacc agcctctcga ctgcgccctt
     2401 tcgggaatca tcgcgctcga ggcggcgcat cacgccgggc cgcacggtcg acatctgcgg
     2461 ccggtgttcc gggcagacga tcgtagccat gatgttgccg ccgaaggcgg gccgcgtcat
     2521 caagaggtta cgcgtaccgt cctctatctc gaggccggtg cagtcggcgg tcaggccagt
     2581 cctgacgcgg gcggacacgc gcggcgccag gtcgcggcct atcgtcgtgg cgccagtgaa
     2641 gacgatgccg ggcttgtagc gctcgatcgc cgcggtcatc gccttggcgt agggctcggt
     2701 catgtagacg tccagggccg gatcgtccac gacgacgacc gtgtcggcgc cctgggctat
     2761 cagctcggcc gccgcgctat cgggcaggcc gtggccgacg agcagggcca ggagcggctc
     2821 ctcgaggtcg tcggctatgc gcctgcccac gccgaggagc tcgtaggcta tcttctgcac
     2881 gatccctcgc cgctgttcgg cgaagaccat cacgccccta taatcggata tgttcattgc
     2941 gtttctcctg tcgtacgccg ccttagatga cgtacttctc cttgagcgcc tcgacgatga
     3001 tcctagcgcc ttctttggcg tcgacctcgt agaccttgcc ggccgatttg acgcccttcg
     3061 tgaaagactt cttgaccctg gtcggcgagc ccttgagtcc gatattgtcc tgatcgacct
     3121 cgatatcggc gaggccccag accttgacct cgcgcctata cgcgtcgaag atgccggaga
     3181 ccgacatata gcgcggccgg ttcatgtcct tgagcgccgt caggaggcag ggcagcttga
     3241 ccttgatacg ctggtaaccg tcctcgaaga cccgcttgac cacgaccgag ccttccgtag
     3301 cctcgagctc gctgacgtag ctgacgtgcg ggaggcccag gtgctcggct atctgcggcc
     3361 cgacctgggc cgtgtccccg tctatagcct ggcgtccgca gataataagg tcgaagccga
     3421 gcttcctgag ggcggccgcg atcgtcgtcg aggtggccca ggtgtccgcg ccgccgaagg
     3481 cccggtcgga cagcagcacg gcctcgtccg cccccatcgc gagcgcctcc cgcagcgcca
     3541 ggtccgcctg cggcggcccc atcgacagca cggtaacctt ggcgcccagc tcgtccttca
     3601 gcgcgagcgc ggcctcgagg ccgcccttgt cgtcggggtt gatgatgctc gggatgccct
     3661 cgcgtatcag ggtgccggtg accgggtcga tcttgacctc ggtggtgtcc gggacctgct
     3721 tcatgcacac tacgatatcc atctcgttcg ttccttccgt ctcgacgcgc ttacttgagc
     3781 atgttggccg agatgaccat gcgctgcacc tccgaggtgc cctcgtagat ctcggtaatc
     3841 ttggcgtc
//
LOCUS       DAOPHV010000214         3837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_32522_length_3837_cov_4.692226, whole genome shotgun sequence.
ACCESSION   DAOPHV010000214 DAOPHV010000000
VERSION     DAOPHV010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32522_length_3837_cov_4.692226"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLQ29_06730"
     CDS             complement(<1..293)
                     /locus_tag="PLQ29_06730"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPG86379.1"
                     /translation="MIDARLEKLAKILVNYSVKVKPGENVLVQDTGIEPAFDRALVKA
                     IHEAGGRAFVSLRDKTLDRALLRDAPPEQVELQARFERERMDAMDCFIGFNAWR"
     regulatory      412..611
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            763..1635
                     /locus_tag="PLQ29_06735"
     CDS             763..1635
                     /locus_tag="PLQ29_06735"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPG86380.1"
                     /translation="MRHGTRQDWRPSPQSTDVLRRRRSVRRAGAAIASVAIHVAALAL
                     AASLIGRPRPTGAALATLAAARPALAVSLSPPRATSQPRPLESELSEPSEPIRSAASD
                     PQDDRPKDAVDTTVDTTVDTTVDTTAGEVAAGLAGPTVDAPEPGAAESAEADPGAVDV
                     EAAREVAAGLVGPPVDAPEPGAAESAEDDPGAVDVEAAGEAAREAAVMARIQAAKAYP
                     DAARRRGIEGEVVVAFAIAADGSLLRVDAEPGPSHVLLEKAARDAIARAAPFPPGDVG
                     PTEYRVTIVYKLER"
     gene            complement(1663..3060)
                     /locus_tag="PLQ29_06740"
     CDS             complement(1663..3060)
                     /locus_tag="PLQ29_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPG86381.1"
                     /translation="MDDSMLDRVFYHIYPLGAFGAPRRNDGRSHPEPRLRRLAEWIPA
                     MERIGADALYLGPVFESESHGYDTLDYLMVDRRLGDGGDLAEASRALASRGIGLYLDA
                     VFNHVGRGHPFVRDVVERGHASPYARWIAGYDPSGPGPGGLPFGYDGWKGHYDLVRLD
                     TGLPEVREYLIGVALRWIDEYGVAGLRLDAADCLDRLFMAELGRRCRLARPGFRLVGE
                     VVHGDAYEPILREGGLDAVTDYEAYKGLWSSYNDRNYHEIAWTLDRLFAEPVAGPASP
                     GTWSGAGLCRGRAMYSFADNHDVDRVASLIRDPAGLYPLYALLFSMPGCPSVYYGSEY
                     GVTGRKAAADDGPLRPALDPAALASAAPHPDLAAAVARFSEARRRSSALRRGSYRRLA
                     VGNLHLAFERRAGDEAVLVAVNASQEPVRLELPAGLPDGPVVDLLDGGYHEESRGGRL
                     ATVVPPHWARWLRKE"
     gene            complement(3073..3654)
                     /locus_tag="PLQ29_06745"
     CDS             complement(3073..3654)
                     /locus_tag="PLQ29_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPG86382.1"
                     /translation="METTRDRILEVAVGAFAARGYEAVGVQELCESSGITKPTLYYHF
                     GSKEGLLAALFAERFGGFNGRVATAAAYPGNRRGDLAGVLLATIGAFVEEARDDPDFS
                     RVRLSSAFCPPSSERYRVARPYLEALYGTIAATFESAALEHGNMAGRAAQYAASFIGA
                     SDAYVGLYLAGDLDPDEAFLRRAIHYYMHGIFS"
BASE COUNT          626 a         1337 c         1335 g          539 t
ORIGIN      
        1 cgccaggcat tgaacccgat gaagcagtcc atcgcgtcca tccgctcccg ttcgaagcgg
       61 gcctggagct ccacctgctc tggcggcgcg tcacggagca gggcgcggtc gagcgtcttg
      121 tcgcgtagcg acacgaaggc gcggccgccg gcctcgtgga tcgccttgac cagggcgcgg
      181 tcgaaggccg gctcgatgcc ggtatcctgc acgagcacgt tctctcccgg ctttaccttg
      241 accgagtaat tgacgagtat cttggccagc ttctcgagcc ttgcgtctat catggggttc
      301 tcctcgcggg cgagtatagc acgggagaga gcatagccag aaagtaccgc ccccgttccg
      361 gcgactagaa acggggccgg gcgtcgcttg acctccgagg cgttagtccg tataacgagg
      421 acacgccaag gttcggcgag cggcccaagc tgcccgccgc caagagggaa tcccgttcaa
      481 atcgggagcg gacccgccgc tgtacccgga tacgacgatc gctcatcgcc actggccctt
      541 ccggggccgg gaaggcgcga ccgccggacg acccggaagc cagaagacct gccaaggcga
      601 tcctttcgcg cggtacgagg tgagccgcta gcgatacgcc ggcgggtagc ctccttgagg
      661 tctccgcatc gggagccgtc cgccctcgtt ctccggggcc cgtccctccc agcgtaacgc
      721 cgacgcccga cctcccccgc cgctccgtac gaggcatcgc ggatgaggca tggaacgagg
      781 caggactggc gcccctcgcc acaatcgacc gacgtcctgc gacgccggcg atcggttcgc
      841 cgggcgggcg ccgcgatcgc ctcggtcgcg atccacgtag cagccctggc gttagcggcc
      901 agcctgatcg ggcggcctcg gccgaccggg gccgcgctgg ccacgctggc cgcggcacgg
      961 ccggcgctcg ccgtatcgct atcgccgccg cgtgcaacct ctcagccgcg cccgctcgag
     1021 tccgaactat ccgaaccatc cgaaccgatc cgttcggcgg cgagcgatcc ccaggacgat
     1081 cggcccaagg acgcggtcga cacaacggtc gacacgacgg tcgacacgac ggtcgacacg
     1141 acggccgggg aggtggccgc ggggctcgca ggaccgacag tcgacgcgcc cgagcccggg
     1201 gcggccgaaa gcgccgaggc cgatcccggt gccgtcgacg tcgaggccgc cagggaggtg
     1261 gccgcggggc tcgtaggacc gccggtcgac gcgcccgagc ccggggcggc cgaaagcgcc
     1321 gaggacgatc ccggtgccgt cgacgtcgag gccgccgggg aggccgccag agaggccgcc
     1381 gtgatggctc gcatccaggc cgccaaggcc tacccggacg ccgccaggcg gaggggcatc
     1441 gagggcgagg tggtcgtggc gttcgccata gccgccgacg ggtcgctact acgggtggac
     1501 gccgagcccg ggccctctca cgtcctgctc gagaaggcgg cccgagacgc gatagcccgc
     1561 gccgcgccgt tcccacccgg cgacgtcggg ccaaccgagt accgggtcac catcgtctat
     1621 aaactggagc gctgagcgcg atgggtgttg cgcggcatag cgctactcct tcctcagcca
     1681 cctggcccaa tgcggcggca ctacggtcgc gagcctgcct ccccggctct cctcgtggta
     1741 tccgccgtcg aggaggtcga cgacgggccc gtcggggagg ccggctggca actccagacg
     1801 tacgggctcc tgggatgcgt tcaccgcgac gagcacggcc tcgtcgccgg cccgccgctc
     1861 gaacgcgaga tgcaggttac ccacggccag gcggcggtag gagccccgcc ggagcgccga
     1921 cgagcggcgc ctggcctcgg agaatcgcgc gacggcggcc gccaggtccg ggtggggcgc
     1981 ggccgaggcc agggcggccg ggtcgagcgc cgggcgtagc gggccgtcgt cggcggcggc
     2041 cttgcgcccc gttacgccgt actcgctgcc gtagtagacg gacgggcagc cgggcatgga
     2101 gaacaagagc gcgtacagag ggtagagccc agccggatcc cgaatcaggc tcgccacgcg
     2161 gtcgacgtcg tggttgtcgg cgaacgagta catggcccgg ccgcggcaca ggcccgcccc
     2221 ggaccaggta cctggagagg ccgggccggc cacgggttcg gcgaacaggc ggtcgagggt
     2281 ccatgcgatc tcgtggtaat tgcggtcgtt atagctcgac cacaggcctt tgtaggcctc
     2341 gtagtcggtc accgcgtcga gcccgccctc gcgcaggatc ggctcgtagg catcgccgtg
     2401 gacgacctcg ccgacgaggc ggaaacctgg cctcgccagc ctgcagcgtc taccgagctc
     2461 tgccatgaaa aggcggtcca ggcagtcggc cgcgtcgagc ctgagcccgg cgacgccgta
     2521 ctcgtcgatc cagcgtagcg cgacgccgat caggtactcg cggacctccg gcaggccagt
     2581 gtccaggcgc accaggtcgt agtggccctt ccacccatcg tagccgaacg gcaggccgcc
     2641 gggcccgggg ccggacgggt cgtagccggc tatccagcgc gcgtagggcg aggcgtggcc
     2701 ccgctcgacg acgtcgcgca cgaagggatg gccgcgcccg acgtggttga acaccgcgtc
     2761 gaggtacagg ccgatgccgc gcgaggcgag ggcccgggac gcctcggcca ggtcgccgcc
     2821 gtcgccgagc cgccggtcga ccatcaggta gtcgagcgtg tcgtagccgt ggctctccga
     2881 ctcgaagacc ggccccaggt acagcgcgtc cgcgcctatc cgctccatcg ccgggatcca
     2941 ttcggccagg cgacgtagcc gcggctctgg gtgcgagcgg ccgtcgttcc gcctcggagc
     3001 gccgaatgcc ccgagcggat aaatatggta gaaaactcgg tcgagcatcg aatcgtccat
     3061 ggtcgctttc ctctacgaga agatcccgtg catatagtag tgaatggcac gccggaggaa
     3121 ggcctcgtca gggtccaggt ctccggctag gtacaggccg acataggcat cggaggcgcc
     3181 gatgaaggac gcggcgtatt gagccgcgcg accggccatg ttaccgtgct ccagcgcggc
     3241 cgactcgaag gtagcggcta tcgttccgta cagcgcctcc aggtacggcc tggcgacacg
     3301 gtaacgctcg ctcgacggcg gacagaaggc cgacgacagc cgcacccgcg agaaatccgg
     3361 gtcgtcgcgc gcctcctcga cgaaggcccc tatcgtcgcc aacaggactc cggcgagatc
     3421 gccgcgcctg ttgcccgggt acgcggcggc cgtcgccacc cgaccgttga agccgccgaa
     3481 acgctccgcg aagagggccg cgagcaggcc ttccttgcta ccgaagtggt agtacagcgt
     3541 cggcttggtt atgcccgatg actcgcatag ttcctggacg ccgaccgcct cgtaaccccg
     3601 ggcggcgaac gcccccacgg cgacttccag tatcctgtct cgcgtcgttt ccatggatac
     3661 gactatatac cgttcggtat attgccgcaa gaccacgaaa cccgttggcg gattgggccg
     3721 ccgcgtctat actactagtt ccgggggcga aggcgatccc cggaacggat cgcgcgttaa
     3781 cgaggagacg atacgtgttc cagaagcaag tgatgatgcg cagggtgctc tacgcgc
//
LOCUS       DAOPHV010000215         3837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_32526_length_3837_cov_3.922263, whole genome shotgun sequence.
ACCESSION   DAOPHV010000215 DAOPHV010000000
VERSION     DAOPHV010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32526_length_3837_cov_3.922263"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="PLQ29_06750"
     CDS             <1..943
                     /locus_tag="PLQ29_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPG86383.1"
                     /translation="IFGEVTPKRIALAHNEGVALALAPFLLVCTVLFKPFVLLVNALS
                     SLVTRLFGDTRPDSLSMETMVQMMSIAEHMGVIDYAKSRMVKNVFRLGSTTVQAVMTH
                     RIDVFSLEGSTNLEDACRLASEAAVSRVPVYVGESENIVGVVAVREAVAELLAGNGHR
                     PLSEFMGEPLFVLPNKTVGDLFALFKKRRETFAVVIDEYGGLAGLVTMRDIVEEIVGE
                     IYEDSDEASRERIERRQDGSYLVSGDAPLSVIAEIAGGEPPNLPYVQTVAGYVAWALD
                     RIAVPGDKAETPLGGFIVTAVDANRVRTAVFRPAGKD"
     gene            947..2104
                     /locus_tag="PLQ29_06755"
     CDS             947..2104
                     /locus_tag="PLQ29_06755"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HPG86384.1"
                     /translation="MIEAGRVVRVGIERIVPGGDGIGRVDGRACFVPLSAPGDLLEAR
                     VVDSRPDYVRAEIVDLLEPGPGRVEPRCPHYGACGGCNLQHLSYEAQLEAKLGLARDA
                     WLRVGRSAEADFTIEPSAPFGYRNRAQFRADASGRPGYARRGSNELLAFDSCPILAEP
                     LERWARSHTAEPGRRFVAFGRDAEPGEAPGSGRAYIEGVDDEATAVVLGRSFRFATAG
                     FFQSNLAMVERLVPAVRSGFSEGAKGDAAADLYCGVGLFGAFLSGSFRRLVCVEQERR
                     SIGYACVNVPRGASFSAMRIEDWTRSAQARERFDLVVVDPPRSGLGPAVRSWLAEARP
                     SALSYVSCDPVSLARDAGFLIDAGYAVESAALYDFYPQTAHIESHVRFRVD"
     gene            2121..>3837
                     /locus_tag="PLQ29_06760"
     CDS             2121..>3837
                     /locus_tag="PLQ29_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPG86385.1"
                     /translation="MTVRAFAAVALMAAALMAAVASQPLYAQGAAAQSAAPTDGAAAE
                     VLFRFPVGGVVSSGPTVAAGRVWLLSDSRTLYTLTVDGVAIGKRGLSERRAAFIVGDA
                     FGRAAVSDGATGLSLINKAGQEAWRVELGARPDSPPAFASDGRLYVSAGGELRAFAPN
                     GRRLWSARPGAAPSSPLVVGPGGGPAIGLADGRVALYHPDSGVATELALGSAPVALAA
                     AGRLAAGLADGRIVVLAAEAGALSPAPGSPAAADAPSLGSRPAALAAASDTFYALGAD
                     GVLLAVDGAGVELWRRRAVTDGSTAALAAFEGRAIVLTRSAVTSYGPDGSVYRSLTLR
                     NAVAMPAIAPNGSVFSGGADWILYAYRFERPLTAAAGPVIEPLDLSAVDAMAKEEAFW
                     SVAPWSDDAAMERLYDIEKSMESGTIGMDSRRAALYLSAVALGRMDAPFGSGRAPTGP
                     APRGPLPRVYACGLLGRMGLPWAVPVLVEAFRGDPEPSVRAAAAYAVATIGLDPEGRA
                     LGAFAEAAGRRLDGATAGAVVDAIDGLYRASGALDDRSGMLALLRIAGGDYARELGPA
                     GIRRVK"
BASE COUNT          477 a         1389 c         1372 g          599 t
ORIGIN      
        1 gatcttcggc gaggtgacgc ctaagcgcat cgccctggcg cacaacgagg gcgtcgcgct
       61 ggccctggcg cctttcctgc tcgtctgcac cgtgctgttc aagcccttcg tgctgctggt
      121 caacgcgctc agctcgctcg tcacgcggct gttcggcgat acccggccgg attccctgtc
      181 gatggagacc atggtgcaga tgatgtccat agccgagcac atgggcgtca tagactacgc
      241 caagagccgc atggtcaaga acgtgttccg cctcggctcc acgacggtcc aggccgtgat
      301 gacccaccgc atcgacgtgt tcagcctcga aggttcgacg aacctcgagg acgcgtgccg
      361 gctggcctcc gaggccgccg tctcgcgcgt gccggtctac gtcggcgaga gcgagaacat
      421 cgtcggcgtc gtggccgtgc gcgaggccgt cgccgagcta ctcgccggca acggccacag
      481 gcccctgtcg gaattcatgg gcgagcccct gttcgtactg ccgaacaaga ccgtcggcga
      541 cctgttcgcg ctcttcaaga agcgccgcga gaccttcgcc gtcgtgatcg acgagtacgg
      601 cggcctggcc ggcctcgtga cgatgcgcga tatcgtcgag gagatcgtgg gcgagatcta
      661 cgaggattcc gacgaggcgt cgagagagcg gatcgagcgg cggcaggacg gctcctacct
      721 ggtctcgggg gacgccccgc tgtcggtcat cgccgagatc gccggcgggg agccgccgaa
      781 cctgccgtac gtgcagaccg tggcggggta cgtcgcctgg gccctcgacc gtatagcggt
      841 gcccggcgac aaggccgaga cgcccctcgg cggcttcatc gtcacggcgg tcgacgccaa
      901 ccgcgtgcgc acggcggtct tccgcccggc cgggaaggac tgagccgtga tcgaggcggg
      961 gcgcgtcgtc agggtaggca tagagcgcat cgtgcctggc ggcgacggca tcggccgcgt
     1021 cgacggccgc gcctgcttcg tgccgctatc ggcgcccggc gacttgctcg aggcgcgcgt
     1081 cgtcgactct cggccggatt acgtccgcgc cgagatcgtc gacctgctcg agccggggcc
     1141 gggccgcgtc gagccgcgct gcccccatta cggcgcgtgc ggcggctgta acctgcagca
     1201 cctgtcgtac gaggcccagc tcgaggccaa gctcggccta gcccgcgacg cctggctgcg
     1261 ggtaggccgt tccgccgagg ccgacttcac gatcgagcca tcggccccgt tcgggtaccg
     1321 caaccgcgcc cagttccgcg cggacgcctc gggccggcca ggctacgctc gtcgcggctc
     1381 gaacgagctc ctcgccttcg actcgtgccc gatactcgcc gagccgctcg agcgctgggc
     1441 gcgctcgcat acggccgagc cggggcggcg cttcgtcgcg ttcgggcggg acgccgagcc
     1501 cggcgaagcg cccggttccg gccgcgccta catcgagggc gtcgacgacg aggctacggc
     1561 cgtcgtcctc ggcaggagct tccgcttcgc cacggccggc ttcttccaga gcaacctggc
     1621 catggttgag cggctcgtgc cggcggtgcg ttccggcttc tcggagggcg ccaagggcga
     1681 cgcggccgcc gatctgtact gcggggtcgg cctgttcggc gccttcctgt ccggatcgtt
     1741 ccgtaggctc gtctgcgtcg agcaggagcg ccggtcgata ggctacgcct gcgtcaacgt
     1801 gcctcgcggc gcctcgttct ccgccatgcg catcgaggac tggacgcgat ccgcccaggc
     1861 ccgcgaacgc ttcgacctcg tcgttgtcga tccgccgcgc tcggggctcg gcccggccgt
     1921 acgctcgtgg ctggccgagg cccgcccttc cgcgctctcc tacgtatcct gcgacccggt
     1981 atcgctcgcc cgcgacgccg gcttccttat cgacgctggc tacgcggtcg agagcgccgc
     2041 gctgtacgac ttctatcctc agaccgcgca tatagagagc catgtcaggt tcagggtcga
     2101 ctagggccgc cgagaggtcc atgaccgttc gagcgttcgc cgcggtcgcg ctcatggcgg
     2161 ccgcgctcat ggcggccgtc gcgtctcagc cgctgtatgc ccagggcgcg gcggcccagt
     2221 cggccgcgcc caccgacgga gccgccgccg aggtcctgtt ccgcttcccg gtcggcggcg
     2281 tcgtcagctc gggcccgacg gtcgcggccg gccgcgtatg gctcctgtcc gattcgcgca
     2341 ccctgtacac cctgaccgtc gacggcgtgg ccataggcaa gcggggactg tccgagcgcc
     2401 gggccgcctt catcgtcggc gacgcgttcg gccgggccgc cgtctccgac ggcgctaccg
     2461 ggctgtcgct gatcaataag gccggtcagg aggcctggcg ggtcgagcta ggggccaggc
     2521 cggactcgcc gccggccttc gcctcggacg gccgcctcta cgtcagcgcc ggcggcgagc
     2581 tccgggcctt cgcgccaaac ggccggcgcc tatggagcgc caggcccgga gccgccccga
     2641 gctcgccgct ggtcgtgggg cccggcggcg gcccggccat cggcctggcg gacggccgcg
     2701 tggcgctcta ccaccccgat tccggcgtcg cgacggaatt ggcgctcggc tcggccccgg
     2761 tagccctggc cgccgccggt cgactggcgg cgggcctcgc ggacggacgc atcgtcgtcc
     2821 tggccgccga agccggcgcc ctatcgccgg cgccaggctc accggccgcc gcggacgctc
     2881 cgagcctcgg ctcgcgcccc gcggccctgg ccgccgcgag cgacaccttc tacgcgctcg
     2941 gcgccgacgg cgtcctcttg gccgtcgacg gggctggggt cgagctatgg cgtcggcgcg
     3001 cggtgacgga cgggagtacg gcggcgctcg ccgccttcga gggccgcgcg atcgtgttga
     3061 cccggtcggc cgttacgagc tacggccccg acggttcggt ctatcgttcc cttacgctca
     3121 ggaacgccgt agccatgccc gcgatagccc cgaacggctc cgtcttctcc ggcggagcgg
     3181 attggatact ctacgcctac cgtttcgagc gaccgctcac ggccgcggcc ggcccggtca
     3241 tcgagccgct cgacctctcg gcggtcgacg cgatggccaa ggaggaggcg ttctggagcg
     3301 tggcgccgtg gagcgacgac gcggcgatgg aacggctgta cgatattgaa aaatccatgg
     3361 aatcgggtac tataggtatg gattccaggc gagcggcgct gtatctttcg gcggtggccc
     3421 tgggccggat ggacgcgcca ttcggatcgg ggcgcgctcc taccgggccc gcgccgcgcg
     3481 gtcccctgcc gcgggtctat gcctgcggcc tccttgggcg catgggcctg ccctgggcgg
     3541 tgcccgtact cgtcgaggcc ttcaggggcg acccggagcc gtcggtcagg gccgcggcgg
     3601 cctacgccgt ggcgacgata ggcctcgacc cggagggccg agccctcggc gccttcgcgg
     3661 aagcggcggg ccggcggctc gacggcgcga ccgccggcgc cgtcgtcgac gcgatcgacg
     3721 gcctgtaccg cgcgtccgga gcgctcgacg atcggtccgg gatgctggcg ttattgagga
     3781 tagcgggcgg cgactacgcc cgggaactcg gccccgcggg catccggcgc gtgaaag
//
LOCUS       DAOPHV010000216         3826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_32664_length_3826_cov_3.616017, whole genome shotgun sequence.
ACCESSION   DAOPHV010000216 DAOPHV010000000
VERSION     DAOPHV010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32664_length_3826_cov_3.616017"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PLQ29_06765"
     CDS             <1..722
                     /locus_tag="PLQ29_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPG86386.1"
                     /translation="FWEVGRDRLGIERVVSYAIEFGFGQATGIDLPGEVGGFVPTPQW
                     KERRFHEKWLGGDTLNLAIGQGFMQTTPLQLADMMAMVVNDGVIYRPHLLKEVRDPRD
                     GSIVTRVVPEVLTASSISKETFKTTKEYLRGVITEGTARYPVNTKAVTVAGKTGTAEV
                     GLADRWHSWFVGYGPVDAADPDDVIVVVAMIEAANPWEWWAPYATNLVFQGYFAGQTY
                     EEAAAQLGLLGRSMPIGQRME"
     gene            722..2041
                     /gene="rodA"
                     /locus_tag="PLQ29_06770"
     CDS             722..2041
                     /gene="rodA"
                     /locus_tag="PLQ29_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPG86387.1"
                     /translation="MASIRKLFSSMDWTIPIAIVILMVIGVLFIYSSGVTSAGDLVST
                     EYAKQIVWAATGLALLVACSLVDLKRVSDYIPIVYGLSLALLAYTLAFGKVVNGARSW
                     LGIIGDYGIQPSEFVKITSALFLARYLDRSVHEASTIKRFALSFAIIAIPMGLILMQP
                     DFGTSLVFVPLYLAMAFIGGINKRYLLSFILVGSIGIVLTVMPLWQTLIASEQSIFLR
                     IFYEKPYDYYILLMLVLVAALSLLGFRFFKKRYYYWLAYGSAIGSLGLAFSLAGQRLL
                     KDYQVKRLIVFLDPSIDPLGSGWNILQSITAIGSGGVAGKGFLQGTQSHYRYLPQQST
                     DFIFSIISEELGFIGGIVIFGLFFYILVRLALSMRKLRDVASSSFVAGVFGIIFFHFM
                     INAGMAMGVMPITGIPLLFLSYGGSSLWSICIGVGLCLGVGARRYDS"
     gene            2038..>3826
                     /locus_tag="PLQ29_06775"
     CDS             2038..>3826
                     /locus_tag="PLQ29_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPG86388.1"
                     /translation="MSRVDPCTLFKTELPKIAKPARYLGGESGSIAKPDAPFRMVLCF
                     PDLYEIGMANNAMRILYAGLNGLDGVACERVFAVAADYERLLRESRTPLYGLETCTPV
                     SSFDILGFTVGYELAATNILSVLDLSGVPLRSADRTDEHPIVIAGGPAITNPTPYSAI
                     LDAVWIGEAEGAFFSLVAELAELKRNGAGRGELLARLRDERSFWMPAAFGTPPKKAVR
                     AIYDGFTEAPYRYAFPMPVVKPVQDHGVVEIMRGCPNGCRFCHAGYFYRPRRLRSPER
                     ILEEVEAQVLEAGHREISLSSLSSGDYPGIAPLVALLNKRWSGYGVSFQLPSLKVESF
                     PLELIEDLSGTRKSGLTFAVETPLEQWQMTLNKRAGIEKISRILAEAEKRGYRVAKFY
                     FMVGLPLPDAGVSEVEAIVSFIGELLAVAPTIKLNLTVATFVPKPHTPFQWSPQLGYV
                     EAASRIYAVKDAFKSNARVKVTYHTPFLSWLEGIVSRGDERVGELLIDAYRRGARFDA
                     WDDLCDKDTWKAALEAAGESATRHMSGFDEAAELPWSDVSLRVSQKFLLDELKRSRDS
                     SLTAACADACSDRCGSCDSSLSVGGDDEIL"
BASE COUNT          672 a         1236 c         1144 g          774 t
ORIGIN      
        1 acttctggga ggtggggcgc gaccgcctcg gcatcgaacg cgtcgtatcc tacgcgattg
       61 aattcggctt cgggcaggcg accggcatag accttcccgg cgaggtcggg ggcttcgtac
      121 cgaccccgca atggaaggag cgccgcttcc atgagaagtg gctcggcggc gacacgctga
      181 acctggccat cggacagggc ttcatgcaga cgacgccgct ccagctggcc gacatgatgg
      241 ccatggtggt caatgacggc gtcatctaca ggccgcacct cctcaaagag gtgcgcgacc
      301 cgcgcgacgg gtccatcgtt acccgcgtcg tgcccgaggt gcttaccgct tcgagcattt
      361 ccaaggagac cttcaagaca acgaaggaat acttgcgcgg cgtcataacc gagggcacgg
      421 cgcgctatcc ggtcaacacc aaggcggtca cggtagccgg taagacgggg acggccgagg
      481 tgggtctggc cgaccgatgg cactcctggt tcgtcgggta cggaccggtc gacgccgccg
      541 atcccgacga cgtcatcgtc gtcgtcgcca tgatagaggc ggccaacccc tgggaatggt
      601 gggcgcccta cgcgacgaac ctcgtattcc agggctattt cgccgggcag acctacgaag
      661 aggcagcggc ccagctaggc ctgctcggcc gctcgatgcc gatagggcag aggatggagt
      721 gatggcgtcg attaggaagc tgttttcgtc catggactgg accataccga tagctattgt
      781 gatactgatg gtcatcggcg tactgttcat ctattcgtcc ggagtgacga gcgccggaga
      841 cctcgtctcg accgagtacg caaagcagat tgtgtgggcc gccacgggcc tggccttgct
      901 ggtcgcgtgc tcgctcgttg acctcaagcg cgtctccgac tatataccga tcgtctacgg
      961 gctctccctg gccctgctcg cctataccct ggccttcggc aaggtcgtga atggcgcgcg
     1021 ctcctggcta ggcatcatag gcgactacgg catccaaccc tccgagttcg tcaagataac
     1081 ctcggccctg ttcctggctc gctacctcga ccgtagcgtc cacgaagcgt cgacgatcaa
     1141 gcgcttcgcc ctatcgttcg ccatcatagc gataccgatg ggcctcatac tgatgcagcc
     1201 cgacttcggc acctcgctcg tgttcgtgcc gctctatctg gccatggcct tcatcggcgg
     1261 aatcaataag cgctacctcc tgtcgttcat tctcgtcggg agcatcggta tcgtgctcac
     1321 cgtgatgccc ctctggcaga cgctcatcgc gtcggagcag tccatcttcc tgcgtatctt
     1381 ctacgagaag ccctacgact actacatact gctcatgctc gtcttggtgg cggcgctctc
     1441 gctgctcggc ttcaggttct tcaagaagcg ctactactac tggctggcct acggttcggc
     1501 tatcggctcg ctcggcctgg ccttctcact ggccggccag cgcctcctca aggactacca
     1561 ggtcaagcgg ctgatcgtat tcctcgaccc gagtatcgac ccgctcggat cgggctggaa
     1621 catactccag tccatcaccg ccataggctc gggcggcgtc gccggcaagg gtttcctaca
     1681 ggggactcag agccattatc gctacctgcc tcagcagagc accgacttca tcttcagcat
     1741 catctccgag gagctcgggt tcatcggcgg catcgtcata ttcgggctct tcttctatat
     1801 cctcgtgcgt ctggcgctat caatgcgcaa actgcgcgac gtggcttcca gctcgttcgt
     1861 cgccggcgtc ttcggcatca tcttcttcca tttcatgatt aacgccggca tggcgatggg
     1921 ggttatgccg atcaccggca taccgctcct attcctgtct tacggcgggt cgtcgctgtg
     1981 gagcatctgc ataggggtgg gactatgcct cggcgtcggg gccaggaggt acgactcttg
     2041 agccgcgtag atccatgcac gttgttcaag accgagctac ccaagatagc caagccggcg
     2101 aggtatctgg gcggcgagag cggctcgatc gccaaacccg acgcgccgtt caggatggtc
     2161 ctgtgcttcc cagacctgta cgagataggc atggccaaca acgcgatgcg tatcctgtac
     2221 gccgggctga acggcttgga cggcgtcgcc tgcgagcgcg tgttcgcggt cgccgccgat
     2281 tacgagcggc tactgcgcga gtcaaggacc ccgctctacg gcctcgagac gtgtacgccc
     2341 gtctcgtcgt tcgatatcct cggtttcaca gtcggctacg agttggccgc cacgaacatc
     2401 ctgtcggtgc tcgacctgtc gggcgtgccg ctacgctcgg ccgaccgaac ggacgaacat
     2461 ccgatagtca tagccggtgg tcctgcgatt acgaacccga cgccgtattc ggccatcctc
     2521 gatgccgtat ggataggaga ggccgagggg gcttttttta gcctcgtcgc cgagctcgcg
     2581 gaacttaagc ggaacggtgc cggccgcggc gagctgctcg cgaggctccg cgatgaaaga
     2641 tcgttttgga tgccagcggc gttcggcacg ccccctaaga aagccgtccg agcgatctac
     2701 gacggtttca ccgaggctcc ctatcgttat gcctttccga tgcccgtcgt caaaccggtc
     2761 caggaccacg gcgtcgtcga gatcatgcgc ggctgcccga acggttgccg cttctgccat
     2821 gccggctact tctatcgccc ccgcaggctc cgctctccgg aacgtatcct cgaggaggtc
     2881 gaagcccagg tgctcgaggc cggtcaccgc gagatcagcc tgtcgtcgct atcgtcgggc
     2941 gactacccgg gcatcgcacc cctcgtcgcc ctgctcaata agcgctggtc cgggtacggc
     3001 gtgtccttcc agctaccgtc gctcaaggtc gaatcgttcc cgctcgaact catcgaggac
     3061 ctgtccggta cccgcaagag cggcctgacc ttcgcggtcg agacgccgct cgagcaatgg
     3121 cagatgacgt tgaacaagcg cgccggcatc gagaagatca gccgtatact tgccgaggcg
     3181 gagaaacgcg gctaccgagt agccaaattc tatttcatgg tggggctgcc gctaccggac
     3241 gcgggcgtgt ccgaggtaga ggccatcgtc tcgttcatcg gcgaattgct agccgtcgcc
     3301 cctacgatca agctcaacct gaccgtcgcg acgttcgtgc caaaaccgca tacgcctttc
     3361 caatggagcc cacagctcgg ctacgtcgag gccgcgtcaa ggatctatgc cgtcaaggat
     3421 gcgttcaagt ccaacgcccg cgtcaaggtc acctaccaca cgccgttcct ttcctggctt
     3481 gagggtatcg tctcccgggg agacgagcgt gtcggagaac tcctcatcga cgcctatcgc
     3541 cgtggcgcac gtttcgatgc ctgggacgac ctctgcgata aggatacctg gaaggcggcg
     3601 ctcgaggccg ccggagagtc ggccacgcgc catatgtccg gcttcgacga agccgccgag
     3661 cttccgtggt cggacgtatc gctgcgggta tcccagaagt tcctcctcga tgagctgaaa
     3721 cgctccaggg actcgtcgct gaccgcggct tgcgccgacg cctgctctga ccgctgcggc
     3781 tcctgcgata gctcactgag cgttggcggt gacgacgaaa tacttg
//
LOCUS       DAOPHV010000217         3819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_32742_length_3819_cov_3.189957, whole genome shotgun sequence.
ACCESSION   DAOPHV010000217 DAOPHV010000000
VERSION     DAOPHV010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32742_length_3819_cov_3.189957"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..412)
                     /locus_tag="PLQ29_06780"
     CDS             complement(5..412)
                     /locus_tag="PLQ29_06780"
                     /inference="COORDINATES: protein motif:HMM:NF025432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HPG86389.1"
                     /translation="MDAERRGALLVILRDRFESNIQRHAGVSWLDVLERLEARPAALD
                     ALGAMEASGGEPDLVAVDGGSGAFVFFDCSPESPAGRRSLCYDRAALDERTEHRPAGD
                     AVSAAGLCSLRSSRAEHRPATPSRRPRRWAPSS"
     gene            complement(402..782)
                     /locus_tag="PLQ29_06785"
     CDS             complement(402..782)
                     /locus_tag="PLQ29_06785"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HPG86390.1"
                     /translation="MRDKPVDVAAYFERFDEPVRRRLEQIRSLVFELLPEATEAIKYG
                     IPTFIHNGNLVHYAGYTGHIGLYPAPSGLERFGDEIARYKRGKGSVRFPLDEELPIEL
                     IRRIVAFRIEEQGGRPRKDGDDGR"
     gene            complement(805..1500)
                     /locus_tag="PLQ29_06790"
     CDS             complement(805..1500)
                     /locus_tag="PLQ29_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HPG86391.1"
                     /translation="MSNELLLVISIVVLYSGLLVWYRLFGERGLYAWTVFSTLAANIE
                     VLILVEAFGMEQTLGNVMFATTFIVTDILSENLGKAASKKAVSIGIATSVSFIVVTQL
                     WLAFSPSPNDWAFESVRTLFSNTPRLMLVSLLVYAVAQRFDVWAYHAIWAVTTRVLGD
                     REKGLWIRNNGSTLVSQLLNTVLYSAGAFWGRYPARTLLSIMASSYLIYVATSLLDTP
                     VVYLARRMHRRGV"
     gene            complement(1807..3138)
                     /locus_tag="PLQ29_06795"
     CDS             complement(1807..3138)
                     /locus_tag="PLQ29_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPG86392.1"
                     /translation="MPRVDPDGAARRLSALIRKRTVSSRDESAIDWTEFEAFRELLPE
                     LYPETHRALELTIVGGHAMLYRWKGRATGEPLVLMAHYDVVPANDEGWERPAFSGDLH
                     EGAVWGRGSIDTKCTLVCAFEAVEALLKAGFTPERDVYLSFGHDEETGGRGAPAIVAH
                     LESLGVKPGLVLDEGGAVVSGVFPGVERPLAMVGVAEKGVTDIELSVRSRGGHSSTPP
                     RKGAAWRLARAITRLEARPFPASFPAATRVMFSRIAPHAPVGLRLVLGNLWLFEPLLL
                     LVFGRAGGELGAMCRTTTAVTMLQGSDAANVLPAVAKAVVNVRVAIGQSVDGVVERMK
                     AVINDPLVELRVLLPGDPSPVSELSGPRFDALSATIAELYPDAVVAPYVVMGGTDARH
                     YARVSSTVYRFSPFELSKAERASMHAVNERLPVASLAKGVEFYLRLIRRAG"
     gene            complement(3144..>3819)
                     /locus_tag="PLQ29_06800"
     CDS             complement(3144..>3819)
                     /locus_tag="PLQ29_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86393.1"
                     /translation="DDMNEATKATTTYDEAIHRNGRAWMAVAIAIILGVPLAISLYLD
                     VWPPLGRLAAALLPTIMVFWPIAIVEVITFTPMLGSGGTYLGFVTGNLSNLKVPAAIA
                     AMQSAGVEPSTKEGEIVSTLAIGASSLVTNAVLLAGVLLMSYLLPVLESPTLKPAFAN
                     LLPALFGALGVVFISRNWKLSIAPLALMIGLFVAMPTAPVGILIPAGAIVSIGVARLL
                     YTRHLL"
BASE COUNT          639 a         1249 c         1338 g          593 t
ORIGIN      
        1 tcggtcagga gctcggcgcc catcgacgcg gccgccgaga cggcgtcgcc ggcctatgct
       61 ccgccctcga cgagcgcaag gagcataggc cggccgccga gacggcgtcg ccggccggcc
      121 tatgctccgt gcgctcgtcg agggcggcgc ggtcgtagca gaggctccgc cgccctgccg
      181 ggctctcggg cgagcaatcg aagaacacga acgcccccga gccgccgtcg accgcgacga
      241 ggtccggctc cccgcccgag gcctccatcg ctccgagcgc atcgagcgcg gcgggcctgg
      301 cttcgaggcg ctcgagcacg tcgagccacg agacgccggc atggcgctgt atgttgctct
      361 cgaatctgtc gcgcagtata acgagcagcg cgcctcgccg ttcagcgtcc atcgtcgcca
      421 tcctttcgtg gccggccgcc ctgctcctct atcctgaagg cgactatccg cctgatcaac
      481 tcgatcggca gttcctcgtc cagcgggaaa cgtaccgagc ccttaccgcg cttatagcgg
      541 gcgatctcgt cgccgaagcg ctcgaggccg ctcggcgccg gatagaggcc gatatggccc
      601 gtatagccgg cgtaatggac gaggttgccg ttatggatga aggtcggtat gccgtacttg
      661 atggcctcgg tggcctcggg tagcagctcg aataccagcg accgtatctg ctcgagcctg
      721 cgccgtaccg gctcgtcgaa gcgctcgaag taggcggcca cgtccacggg tttgtccctc
      781 atcggtatgc cgcccgtccg agtctcatac gcccctccta tgcatgcgcc tggccaggta
      841 gacgacgggc gtatcgagca ggctcgtcgc cacgtagata agataactcg aggccatgat
      901 ggagagaagg gtcctggccg gatagcgtcc ccagaaggcg cccgcggagt acagtacggt
      961 attgaggagc tgcgagacga gggtggagcc gttgttcctg atccacaggc ccttctcccg
     1021 gtcgccgagt acacgggtcg tcacggccca gatggcgtgg taggcccaga cgtcgaagcg
     1081 ctgggcgacg gcgtagacga ggaggctgac gagcatgagc cgcggggtgt tcgagaacag
     1141 ggtcctgacg ctctcgaagg cccagtcgtt aggcgacggg ctgaaagcga gccacagctg
     1201 ggtcacgacg atgaagctga ccgaggtggc gatgccgatg gacacggcct tcttcgacgc
     1261 ggccttgccc aggttctcgc tcaggatgtc ggtgacgatg aaggtggtag cgaacatcac
     1321 gttgccgagg gtctgttcca tgccgaaggc ctcgacgagt atcagcacct cgatgttggc
     1381 ggccagggtc gagaagacgg tccaggcgta cagcccccgc tcgccgaaga ggcggtacca
     1441 gacgaggagc cccgagtaca ggacgacgat ggatatgacc agtaacagct cgttgctcat
     1501 gcgttcgctc cgtgtcgcgc agagccagac ggatcgacga aacgtgctag agcccgccat
     1561 cctcgcccgg ctcgggcgcg gaggactata caggcgccgg gatggcgcct gtacgcccgg
     1621 taggcaaccg ccgccggggg acctagtttt gtttaacgac aggagggttt cgaactgtcc
     1681 tatggctctg gcgtgtaata gatcgccccg cgcgccgtgc ggccgcgagg cgggcacgat
     1741 agcacggcgg ggctgagcgg cgcaaccgcc acaccgtcgt cccgccgcgc ctcgttgttt
     1801 tcccgattat ccggcccgac ggatgaggcg caggtagaac tcgactccct tggcgagcga
     1861 ggcgaccggc aggcgctcgt tgaccgcgtg catgctggcg cgctcggcct tggacagctc
     1921 gaacggcgag aagcggtaga cggtcgacga gactcgggcg tagtgcctgg cgtcggtgcc
     1981 gcccatcacc acgtagggcg cgacgaccgc gtcggggtac agctcggcta tcgtcgccga
     2041 cagcgcgtcg aagcgagggc ctgacagctc cgataccggc gacgggtctc cgggcagcag
     2101 cacgcgcagc tcgacgaggg ggtcgtttat cacggccttc atgcgctcga cgacgccgtc
     2161 gacgctctgc ccgatggcga cgcgcacgtt gacgacggcc ttggctacgg cgggtagcac
     2221 gttggcggcg tcggagccct ggagcatcgt cacggccgtc gtggtgcggc acatcgcgcc
     2281 gagttcgccg ccggcccggc cgaagacgag gaggaggagg ggctcgaaca gccagaggtt
     2341 cccgagcacc aggcgcagtc cgaccggcgc gtgcggggct atcctcgaga acatgaccct
     2401 cgtcgctgcc gggaagctgg ccgggaaggg cctggcctcg agccgggtga tggcgcgggc
     2461 gagccgccag gccgcccctt tgcgcggcgg ggtcgacgag tgcccgcccc ggctgcgcac
     2521 ggacagctcg atgtcggtga cgcccttctc ggcgacgccg accatcgcga gcggccgctc
     2581 gacgccgggg aacacgccgc tgaccacggc gccgccttcg tcgagcacca ggccaggctt
     2641 gacgcccagg ctctcgagat gggcgacgat ggccggcgcg ccccggccgc cggtctcctc
     2701 gtcgtgcccg aaggacaggt acacgtcgcg ctcgggcgtg aagccggcct tgagcagggc
     2761 ctcgacggcc tcgaaggcgc agaccagcgt gcatttcgtg tcgatggagc cgcggcccca
     2821 gaccgctccc tcgtgaaggt cgccggagaa ggcgggccgc tcccagcctt cgtcgttcgc
     2881 gggtaccacg tcgtaatggg ccatcaggac gagcggctcg cccgtggccc tgcccttcca
     2941 gcggtacagc atcgcgtggc cgccgactat tgtcagctcg agcgcccggt gcgtctcggg
     3001 gtacagctcc gggaggagct cgcggaaggc ctcgaactcg gtccagtcga tagctgactc
     3061 gtcgcggctc gagacggtgc gcttgcgtat cagggcggac aggcgcctgg ccgcgccgtc
     3121 tggatctact ctcggcatat tgttcatagc aggtgccttg tatataggag acgcgccacg
     3181 cctatcgaca cgatggcgcc ggccgggatc aggataccga cgggagcggt cggcatcgcg
     3241 acgaacaggc ctatcatcag cgccaggggc gctatcgaca gcttccagtt cctcgagatg
     3301 aacaccacgc cgagggcgcc gaacagggcc ggtagcaggt tggcgaaagc cggcttcagg
     3361 gtcggcgact cgagcacggg caggaggtac gacatcagga gcacgcccgc gaggagcacc
     3421 gcgttggtca cgagcgacga ggcgccgatg gcgagcgtcg atacgatctc accctccttg
     3481 gtcgacggct cgaccccggc ggactgcatc gcggctatcg cggccggcac cttgaggttc
     3541 gacaggttgc cggtgacgaa gccgaggtag gtgccgcccg agccgagcat cggggtaaag
     3601 gtgatcacct cgacgatggc gatcggccag aagaccatga tggtcggcag gagggccgcg
     3661 gcgagcctgc ccaggggcgg ccacacgtcg aggtacagcg atatggccag cggtacgccg
     3721 agaatgatcg cgatggcgac cgccatccag gcccggccgt tccggtggat ggcctcgtca
     3781 taggtcgtcg tcgccttcgt cgcttcgttc atatcgtcc
//
LOCUS       DAOPHV010000218         3814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_32809_length_3814_cov_3.420059, whole genome shotgun sequence.
ACCESSION   DAOPHV010000218 DAOPHV010000000
VERSION     DAOPHV010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32809_length_3814_cov_3.420059"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLQ29_06805"
     CDS             complement(<1..278)
                     /locus_tag="PLQ29_06805"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPG86394.1"
                     /translation="MPKQSSIRNAADVVTVLGGSTSFSGTLKFDTSLMIRGSFDGDID
                     AKGALYIDEGATVNVGRIRAMSIVVAGSVRGDIEAVDKVELRSSAQVRG"
     gene            complement(294..515)
                     /locus_tag="PLQ29_06810"
     CDS             complement(294..515)
                     /locus_tag="PLQ29_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG86395.1"
                     /translation="MISEDKRRVLDLFAEGRKLYKLMRFSDAADKFDEALKIDPTDGP
                     SKVYLDRCDHYVIDPPDEDWDGVFVMKHK"
     gene            complement(541..1206)
                     /locus_tag="PLQ29_06815"
     CDS             complement(541..1206)
                     /locus_tag="PLQ29_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPG86396.1"
                     /translation="MSNKKRILVVDDEQINLEFFDVMLSKLGFAVHKAENGQEALEAV
                     RRLRPDLIILDNIMPKLSGWEVTKIIKSSPEYAEFSEIPIIMFSALDDVKDKVEGLEL
                     GADDYITKPFNFAEVLARIRAILRSYELVKQIENREQSIIVGERAVAEMQKAIASTKR
                     SLEQIGSASDLVTAKELAAKTRAALVELDSRFGSFSSEYTELRNKASDIKTLRGKPHR
                     AES"
     gene            complement(1320..2942)
                     /gene="lnt"
                     /locus_tag="PLQ29_06820"
     CDS             complement(1320..2942)
                     /gene="lnt"
                     /locus_tag="PLQ29_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPG86397.1"
                     /translation="MQPKRRANSTAAVAPPPHLTSNLALLALTILLYTASFYSGSSLA
                     PFAAFFALVPAMAAAEGLGPLRSAIAGALSGAVALSIPCSWLASYHPVAITLVAFVGA
                     WSFSAAFAAVSAILRTGHPAAPLAVALVWASADIARSVGFLAFPYGSLPYALAGNRVA
                     LGLASIGGAPLVALAIAGANASCYAAARAIAHHGPGGRLRAALAALPAAAIVGAAVFG
                     AAPPFFSRPDDSLVVASPNPAGEPVAGSFRVALVQPCVYEQRSAPAYAAAFRKLAALS
                     VEAAERGADLVAWHETAVVPPIAWHLKQRPDRLTYEFVSDVDRFLSAYPVPLLVGNGY
                     ADPDDRARRVEHNSALLFVSGAAIARYDKAKLVPFSEYMPAWFGRALPSLDRRVIATF
                     GPYWTPGSEAGLIELGDARFAAPICFEDSFARYFASFEEPDFFIVLTKDSWAGSASMQ
                     AQHLAMSRYRAAETGSIVLRVADTGSTAAIAPDGAVVGELAPFEHGVLYADVTLAAGT
                     RTAYEAGGMYFDLAIPLLAALLSTLCARRRPR"
     gene            complement(2917..3258)
                     /locus_tag="PLQ29_06825"
     CDS             complement(2917..3258)
                     /locus_tag="PLQ29_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPG86398.1"
                     /translation="MARKVTKAELIDALHERSNLGRKDIHILIDGLFEEIKKAILEEK
                     AVELRGFGTFEVRIRKGRKRARNPKTGEPVSVSDHGVAVFRPGRELKQEAWKISAIAS
                     PPPDAAEASGE"
     gene            complement(3359..3640)
                     /gene="rpsT"
                     /locus_tag="PLQ29_06830"
     CDS             complement(3359..3640)
                     /gene="rpsT"
                     /locus_tag="PLQ29_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPG86399.1"
                     /translation="MEDRLATKFSSAEKRHKQDEKRRVQNKKVKSSVRTAAKRVVTAA
                     HAKDKDLAEASLKDMIKQIDTAARKGIIKKNAAARKKSRMQRLVNVLNA"
BASE COUNT          664 a         1138 c         1246 g          766 t
ORIGIN      
        1 ccgcgtacct gggcgctcga gcgtagttcc accttgtcga cggcttctat gtcgccgcgg
       61 acggagcctg cgacgacgat gctcatcgcc cgtatcctcc ctacgttcac cgtcgcgccc
      121 tcgtcgatat agagggcgcc cttcgcgtcg atgtcgccgt cgaagctacc gcggatcatg
      181 aggctcgtgt cgaattttag cgttccggag aagctcgtcg agcctccgag tacggtcact
      241 acgtccgccg cgttgcggat cgatgattgt tttggcatcc aggcctcggc tccctatttg
      301 tgcttcatga cgaagacgcc gtcccaatcc tcgtcgggcg gatcgatcac gtaatggtcg
      361 catcggtcga gatacacctt cgacggaccg tcggtcgggt cgatcttgag cgcttcgtcg
      421 aatttatctg ccgcgtcgga gaagcgcatc agcttataca acttcctgcc ctcggcgaac
      481 aggtcgagga cgcgtctctt gtcttcgctg atcataccgg ctccttccgc cccgccgtcg
      541 ctacgactcg gcgcgatggg gcttgcctcg tagcgtcttg atatcgctcg ccttgttgcg
      601 gagctctgtg tactcggagg agaacgagcc gaacctggag tcgagctcca ctagtgcggc
      661 cctcgtcttg gccgccagct ccttagctgt aaccaggtct gacgcggacc ctatctgctc
      721 gagcgatcgt ttcgtcgagg ctatcgcctt ctgcatctcg gctacggccc tctcgccgac
      781 gatgatgctc tgttcccggt tctctatttg ttttacgagt tcgtacgagc gcaggatggc
      841 ccggattctg gccaggacct cggcgaagtt gaacggcttt gtaatgtagt cgtctgctcc
      901 gagctcgagc ccttctacct tgtccttgac gtcgtcgagg gccgagaaca tgatgatggg
      961 gatttccgag aattctgcgt actccggcga cgacttgatg atcttggtca cttcccagcc
     1021 cgacaacttg ggcatgatgt tatcgaggat gatcaggtcg gggcgcaggc gcctgacggc
     1081 ctcgagggct tcctgcccgt tctccgcctt atggaccgcg aacccgagct ttgacagcat
     1141 tacatcgaag aactcgaggt ttatctgctc gtcgtcgacg acgagtatgc gttttttatt
     1201 gctcacgtgg aaccccgttc gccgttctgg atcgatcgcc gagcgcgcga ggccatataa
     1261 gccggcgcca tgatacggcc attttcctat acggatcgct tcttgtcaat gcgcaagccc
     1321 tatcggggtc gtcgacgtgc gcagagtgtg gataggagcg cggcgaggag gggtatggca
     1381 aggtcgaaat acatgccgcc ggcctcgtaa gcggtccggg tcccggccgc aagcgtcacg
     1441 tcggcgtaca gcactccgtg ctcgaacggc gccagctcac ccacgacggc gccgtccggc
     1501 gcgatggccg cggtggagcc ggtatcggct actcgtagca cgatcgagcc ggtctcggcc
     1561 gccctgtacc tcgacatggc gaggtgttgc gcctgcatcg aagccgaacc agcccaggag
     1621 tctttcgtga gtacgatgaa gaagtccggt tcttcgaatg aggcgaagta cctggcgaat
     1681 gagtcctcga agcagatggg ggcggcgaag cgggcgtcgc ccagttcgat gagcccggct
     1741 tcggagccgg gcgtccaata tgggccgaag gtcgctatga cgcgtcggtc gagcgacggc
     1801 aaggctcgac cgaaccatgc cggcatgtac tccgagaacg gtacgagctt ggccttgtcg
     1861 tacctggcta tggccgcccc ggagacgaag agcagggccg agttatgctc gacgcgcctg
     1921 gcgcggtcgt cgggatcggc gtagccattc ccgaccagta gcggtacggg gtaggcagac
     1981 aggaatcgat cgacgtccga aacgaactcg taggttaggc gatctggcct ctgcttgagg
     2041 tgccaggcta tgggggggac gacggccgtt tcgtgccagg cgaccaggtc cgcgcctcgt
     2101 tcggccgctt cgacgcttaa ggctgctagc ttcctgaagg cggcggcgta ggccggcgcg
     2161 gatcgctgct cgtagacgca gggctgtacg agagcgaccc gaaacgatcc ggccaccggc
     2221 tcgcctgccg gattggggct cgcgacgacg agactatcgt ctggcctcga gaagaacgga
     2281 ggggccgcgc cgaacacggc ggctccgacg atggctgcgg ctggtagagc cgcaagcgcg
     2341 gcccgtaggc ggccccccgg accatggtgc gcgatggccc gagcggcggc gtagcagctg
     2401 gcgttagcgc cggcgatcgc cagagctacc agcggcgctc cgcctatcga ggcgaggccc
     2461 agggccactc ggttgccggc aagtgcgtag ggcaggctac cataggggaa ggccaggaag
     2521 ccgaccgatc tggctatgtc ggcgctcgcc cagacgagcg cgacagcgag cggcgccgca
     2581 gggtggccgg tacgcaggat ggcggagacg gcggcgaagg cggccgagaa cgaccacgca
     2641 ccgacgaagg cgacgagtgt tatcgctacg gggtggtacg aggcgagcca ggagcagggt
     2701 atcgatagcg cgacggcgcc cgacagggct ccggctatgg ccgaccgtag cgggccaagt
     2761 ccttctgccg cggccatagc tggtacgagg gcgaagaacg ccgcgaacgg cgcgagcgac
     2821 gagccgctgt agaacgaggc ggtatagaga agtatagtca gggcaaggag cgcgaggtta
     2881 ctggttagat gtgggggagg ggcgaccgcg gcggtactat tcgcccgacg cttcggctgc
     2941 atcgggcggt ggggaggcta tggcgctgat tttccaggcc tcctgcttga gctcgcgacc
     3001 gggcctgaaa acggccacgc catgatccga taccgatacg ggttcgccgg tcttgggatt
     3061 acgagcccgc ttacggccct tacggatgcg cacctcgaag gtaccgaacc cgcgcaactc
     3121 cacggccttc tcttctaaga tagccttctt gatctcttcg aacaggccgt cgatgaggat
     3181 gtgtatgtct ttacggccga ggttcgagcg ctcgtggaga gcgtctatca gttcggcttt
     3241 cgtgactttt ctagccatgc gacgactcct gctgaatcgg accgtccggg gaccctatct
     3301 cggagaggcc cgaaggcctc cctataccga tggagcggcc ggccgcgggg taaggcgatt
     3361 aagcgttgag gacgttgacg agacgctgca tcctggactt cttgcgggcg gcggcgttct
     3421 tcttgatgat gcccttgcgc gcggcggtgt cgatctgctt gatcatatcc ttgagagagg
     3481 cctcggcgag gtccttatcc ttggcatggg cggcggtgac cacgcgcttg gcggcggtgc
     3541 ggacgctgct cttgaccttc ttgttctgga cgcgtcgctt ctcgtcctgc ttgtgccgct
     3601 tctcggcgga tgaaaatttg gtagccaatc tatcctccat tcggaatgtc gcaactctat
     3661 gtctattaga caggaattaa cgtcggtaga agaaattacc agatcggcta gagtatgtca
     3721 aggatatcct gggccagccc gcgcttattg ccgagcgacc ggcgaaccga tatagtcgtt
     3781 cccatgggaa ccatcctgct agccctgacc tcgc
//
LOCUS       DAOPHV010000219         3813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_32814_length_3813_cov_4.418042, whole genome shotgun sequence.
ACCESSION   DAOPHV010000219 DAOPHV010000000
VERSION     DAOPHV010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32814_length_3813_cov_4.418042"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLQ29_06835"
     CDS             complement(<1..210)
                     /locus_tag="PLQ29_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86400.1"
                     /translation="MAIYETLAKAYDELFPPAPEAAAFIGRAIGSSEQRNEVDDRPWR
                     NGAGRAGAPAPTRGKPAIDLGCATGA"
     gene            298..645
                     /locus_tag="PLQ29_06840"
     CDS             298..645
                     /locus_tag="PLQ29_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86401.1"
                     /translation="MKLLVLVLNDDKHLHGVMEAYVEAGVTGATILDSEGMGRFLAYQ
                     VPLFADFKDFMQGNKPSNKTILSVVRDERVLERLKDLVGRVIGGFSTPGAGVMFTVPV
                     DWFAGPERDEGDD"
     gene            647..1108
                     /locus_tag="PLQ29_06845"
     CDS             647..1108
                     /locus_tag="PLQ29_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPG86402.1"
                     /translation="MPALRNALAPDRAAVGVKAADKREAIAALVDLLAAAGKAPDRDA
                     LLAAVLEREALAPTGLGDDCAIPHAQTDAVSETSVAVVRLAEPVGFDAPDGSPARLVF
                     LLAGPKDSAAVHLRLLSKLARLLSDPEFRRALLEAPDGAALAGLIIGADQA"
     gene            1204..1285
                     /locus_tag="PLQ29_06850"
     tRNA            1204..1285
                     /locus_tag="PLQ29_06850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1238..1240,aa:Leu,seq:gag)
     gene            1593..2105
                     /locus_tag="PLQ29_06855"
     CDS             1593..2105
                     /locus_tag="PLQ29_06855"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HPG86403.1"
                     /translation="MTNHRSSIRFACAAIEAMLVALIASCSSVPAPAASCGEAVSPAD
                     LSGTAWLLTGYRYGDRSVALDRASMDAAGDGDAFSLSFDGGRVGGVASCNRYFGEAAS
                     GGPGGLSITGLASTKMACLDEPYGLSEYEFFVALSVATGWDLANGSLMLGGRAADGSS
                     VELAFSSPGR"
     gene            2124..3434
                     /locus_tag="PLQ29_06860"
     CDS             2124..3434
                     /locus_tag="PLQ29_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPG86404.1"
                     /translation="MKFVTKHFYTVFVASMAILCAASAGTAFWAVRRAEGMNAALVQE
                     RIYEFKRTFLRDTVRNMIRDIDRLRDYNLRRAREDLDPLTDEAWADRETKATIADVIR
                     AKSFEHDGYIWVNEVLDWSGGDGYAVRRVHPNLVDTEGCLLSTKATDIKGNTPYLTEL
                     EGVREHGELFYRYYFKRMGSDEVSEKLTYAALYPDYDWIVAMGMHVDDIETYTLAVRE
                     AASDFNARVIALVVVLLVLFFGLGVALLIVAGRRFMDASARAIRTEANIDPLTGALNR
                     RIGDRYFSDAFRAFRYGKPSPLVLSIDLDDFKRVNDAYGHDAGDVVLRAVVERVKQTM
                     RASDFLFRWGGEEFLLAYSGVSPDEAARLADRLNRAVASTPIRLRPAEPAGSPELVVS
                     VSVGVSWFGHADGSYADAIGRCDRALYRAKAAGKDRAFVEPPGA"
     gene            complement(3442..3708)
                     /locus_tag="PLQ29_06865"
     CDS             complement(3442..3708)
                     /locus_tag="PLQ29_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86405.1"
                     /translation="METYFSPLESALADLPMPPRQLYILASRKLLGGRDDGFVAAYAA
                     ELLALDAAGKAERGYVQILETCIEAFGRASNAARGRERRGPVPA"
BASE COUNT          601 a         1313 c         1263 g          636 t
ORIGIN      
        1 ggcgccggtc gcgcagccga ggtcgatggc cggtttgccg cgcgtaggcg cgggggcgcc
       61 ggctcggccg gcgccgttcc gccatggcct atcgtcgact tcgttcctct gctcagacga
      121 acctatggcc cggccgatga aggccgcggc ttcgggcgcc ggcgggaaga gctcgtcata
      181 ggccttcgcg agcgtttcgt agattgccat ggatccaaga tactcacggg cggcttccgg
      241 gcaaacagcg tttgacaaag cggccgattc gtcgatactg gacgcggagg cgccacggtg
      301 aaactgctcg tattggtcct gaacgacgac aagcatctgc atggggtgat ggaagcctac
      361 gtcgaggccg gcgtgaccgg cgccactatc ctcgattccg agggcatggg tcgtttcctg
      421 gcctatcagg tgcccctgtt cgccgacttc aaggatttca tgcaaggtaa caagccgagc
      481 aataaaacca tcctatcggt cgtgcgggac gaacgcgtcc tcgagcggct caaggacctc
      541 gtcggacgcg tcataggcgg cttctcgacg ccgggcgccg gcgtcatgtt caccgtgccg
      601 gtcgactggt tcgccggtcc cgagcgggac gagggggacg actaacgtgc cggccctgcg
      661 aaacgccctg gcgccggacc gcgccgccgt cggcgtcaag gcggccgaca aacgcgaggc
      721 gatagccgcc ctcgtcgacc tgctcgcggc cgccggcaag gcgcccgacc gcgacgccct
      781 cctagcggcc gtgctcgagc gcgaggccct ggctccgacc ggcctcggcg acgactgcgc
      841 catcccccac gcccagaccg acgccgtctc ggagacctcg gtagccgtcg tgcgacttgc
      901 cgagccggta ggcttcgacg cgccggacgg cagcccggcc aggctcgtgt tcctgctcgc
      961 cggccccaag gactcggcgg ccgttcacct gcggctcctg tccaagctgg ccaggctcct
     1021 atccgatccc gagttcaggc gagccctgct cgaggccccc gacggcgccg cccttgccgg
     1081 cctgatcatc ggcgccgacc aggcctgagg aaccggaccc gaggcccggc ttgacatgcg
     1141 cgccgccttt caggtataaa ccttccgtca agcgctccct cagggacgcc cgacgaactg
     1201 cctgcggcga tggtggaatt ggcagacacg ctagcttgag gtgctagtgc cgagaggcat
     1261 ggaggttcaa gtcctcttcg ccgcacaaaa caaaaacccc gaccgaaagg tcggggtttt
     1321 tgttttggat cagcggcgga gaggacttgt agcgcggccg gcggccgtcc aacctattcc
     1381 tctcaatcgc gcgcgaagcg cgctagctct gcgtatcggg caggcccgcg attgacctgc
     1441 gccagccagg ccgaatcgcg gcccgcgatc gccgcgatga gcccgcgatc gccgcgatga
     1501 gcccgcgatc gccgcgatga gcccgcgatc gcgtgcttcg ccgccgcatc gatatggcga
     1561 ccaccgcccc tcttgctgta tacttatcgc gtatgactaa tcatcgttca tctattcggt
     1621 tcgcgtgcgc cgccatcgag gccatgctgg tcgctctcat cgcctcctgt tcctccgttc
     1681 cagcgccggc ggcaagctgc ggcgaggccg tatcgccggc cgacttgtcc ggaaccgcat
     1741 ggcttctgac cggctatagg tacggcgacc ggtccgtggc gctggacaga gcgtcgatgg
     1801 acgcggccgg agacggtgac gcgttcagcc tgtcgttcga cggcggcagg gtagggggcg
     1861 tcgcctcgtg caaccgctat ttcggcgagg cggcctcggg cggccccggg ggcctctcga
     1921 taacgggcct cgccagcacc aagatggcct gtctcgacga accgtacggc ctctccgagt
     1981 acgaattctt cgtggcgctg tcggtcgcca cgggctggga tctggctaac ggatcgctca
     2041 tgctcggcgg acgggccgcc gacggctcgt cagtcgagct ggccttctcg tcgcccgggc
     2101 gatagccagg cgaccgacgc ccgatgaaat tcgtcacgaa gcatttctat acggtattcg
     2161 tcgcctcgat ggccatactc tgcgcggcga gcgcaggaac cgccttctgg gcggtccgcc
     2221 gcgccgaggg catgaacgcg gccttggtcc aggagcgcat ctacgagttc aagcgtacgt
     2281 tcctgcggga tacggtgcgg aacatgatac gcgatatcga ccgcctgcga gactacaacc
     2341 tgcggcgcgc ccgggaggat ctggatccgc tgacggacga ggcctgggcc gaccgcgaga
     2401 ccaaggcgac gatagccgac gtcatccgcg ccaagtcgtt cgagcacgac ggctatatct
     2461 gggtcaacga ggtgctcgac tggtcgggcg gcgacggcta cgccgtcagg cgcgtccacc
     2521 cgaacctcgt cgataccgag ggctgcctcc tgtcgacgaa ggccaccgat atcaagggca
     2581 acaccccgta cctgaccgag ctcgagggcg tcagggagca tggcgagctg ttctaccgat
     2641 actacttcaa gcgcatgggt agcgacgagg tatccgagaa gctgacctac gcggcgctct
     2701 atcccgacta cgactggatc gtggctatgg gcatgcacgt cgacgatatc gagacctata
     2761 ccctggccgt gcgtgaggcc gcctcggact tcaacgctcg cgtgattgcg ctggtcgtcg
     2821 tcctgctcgt gctgttcttt ggcctcggcg tggccctgct catcgtggcg ggccgcaggt
     2881 tcatggacgc ctcggcccgg gcgatacgca cggaggccaa tatcgatccg ctgaccggcg
     2941 ccctgaaccg ccgcataggc gaccgctact tctccgacgc cttcagggcc ttccgctacg
     3001 gcaagccgtc gcccctcgtc ctgtccatcg acctggacga cttcaagcgc gtcaacgacg
     3061 cctacggcca tgacgcgggc gacgtcgtgc tccgagccgt cgtcgagcgc gtcaagcaga
     3121 cgatgcgcgc gagcgatttc ctgttccgct ggggcggcga ggaattcctc ctggcgtact
     3181 cgggcgtctc gcccgacgag gcggccaggc tcgccgacag gctcaaccgc gccgtcgcgt
     3241 cgaccccgat acggctccgg ccagcggaac cggcggggtc tcccgagtta gtcgtcagcg
     3301 tatcggtcgg cgtctcctgg ttcggccatg cggatggctc gtacgccgac gccatcggcc
     3361 gctgcgaccg ggccctctac cgggccaagg cggccggcaa agaccgtgcc ttcgtcgagc
     3421 ccccaggggc ctaggcggcc ctcatgccgg cacggggccc cgtcgctcgc gtccgcgagc
     3481 cgcgttcgaa gcccgcccga aggcctcgat gcaggtctcg aggatctgca cgtagccgcg
     3541 ctcggccttg cccgcggcgt ccagggccag gagctcggcc gcgtaggcgg ccacgaagcc
     3601 gtcatcgcgg ccgccgagca gcttgcgcga cgcgaggatg tacagctgcc ggggcggcat
     3661 cggcaggtcg gccagcgccg actcgagcgg gctgaaatag gtttccatcg gcgacctcct
     3721 ggccgcgcgt cggcggctac taccactatc ggcgcgatcc gggtcgcggt gaagggcgct
     3781 cgccttaggt cgacgatatg cccgcacgaa aag
//
LOCUS       DAOPHV010000220         3807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_32894_length_3807_cov_3.473081, whole genome shotgun sequence.
ACCESSION   DAOPHV010000220 DAOPHV010000000
VERSION     DAOPHV010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32894_length_3807_cov_3.473081"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="PLQ29_06870"
     CDS             <1..1118
                     /locus_tag="PLQ29_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86406.1"
                     /translation="RGFFWFFFERNDSPNLVVEDSKFPMQRLSSGRKGVYLYRVRAHA
                     DRLSLEMCHILTDGYGAMIYFRSLLAEYFRLKGVTTEYGPMVFAPNDAPGPEEWEDAF
                     SRYATPGAPGPAKLGPAWHLPGIGLPVHTMRVVTGELSLSSALAKAKEHGATLTIYLS
                     SVFLAALQDVQDAEYEGRPRSRRRPLRLQVPANLRNFFPSSTLRNFSLYALPELDTRL
                     GHYEFSEIVARVKATMALAFTPKEIMRTITRNVATAKHPVIRAVPLTMKNLIMRGLYR
                     SHGEALYSSLSTNVGQARLPDSFASKVKRIDVVLPSTPGLKTVAGMLSHGDALSISFG
                     SVIERRDLERAFFTRLVRDGLKVRVESNLGAPEGKDA"
     gene            1119..1856
                     /locus_tag="PLQ29_06875"
     CDS             1119..1856
                     /locus_tag="PLQ29_06875"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HPG86407.1"
                     /translation="MPYCSRCGVEVDDATSDCPLCEAPIQRLDAPASPSGAGPYPQHI
                     IDPQDRYRLSRAERRRIAIEILSLVAILGSGALLLMDLLSSADLGWSRYAVASIVFGW
                     CAACLPIALYGSPKAAIAAVGAASVAFLVALDTMDGAIGWSLSLGAPIALASFAAVSA
                     TAACIATRRVKGLNLLGIGAIGLAAYLVALEALLRIGLLGLERFGEVRPYWSLVAALA
                     LVPVAVFLFYLHGRVARGADLRKIFRL"
     gene            complement(1860..2555)
                     /locus_tag="PLQ29_06880"
     CDS             complement(1860..2555)
                     /locus_tag="PLQ29_06880"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPG86408.1"
                     /translation="MKKTIVAFAAIAALSFSLVSCADKADSAKDAAASAADASYAFGL
                     AIGTSLKDTGVELDYDEFLKGMKDYIEDREPRMSMETAQMTIQSAIMQAMQAIASRNA
                     EAEAAFLAENGKKSGVTTTASGLQYEVIKLGSGPKPAESDTVRVDYVGTFLDGKEFDS
                     SKNAGEPVVFPVAMVIPGWVEGLQLMPVGSKYKFYIPSALAYGEQGSQGGIEPNKTLV
                     FEVELLGIEAAAQ"
     gene            complement(2677..3390)
                     /locus_tag="PLQ29_06885"
     CDS             complement(2677..3390)
                     /locus_tag="PLQ29_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPG86409.1"
                     /translation="MNEEIPIVFMDESILVISKPAGILSFPDRYEPDARVAARELEME
                     YGRLWPVNRLDRDASGVQLLARDEAGWRLLCARLEDREVTTIYHAVVAGRPTWSDSSC
                     DLSLTADGDRMHRTVIDGSGKPALTDFAVLGCHAKMAIVEARPLTGRAHQVRVHLAAL
                     GYPVACDPLYGDGKPVFLSKIKRRWKGDEDRERPLISRTALHAFSIAFAHPLTGEAVR
                     VEAPYPKDFGALVKQLRKL"
     gene            3636..>3807
                     /locus_tag="PLQ29_06890"
     CDS             3636..>3807
                     /locus_tag="PLQ29_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HPG86410.1"
                     /translation="MAKQLMFSEDARKKLLSGVEQISRAVKVTLGPKGRNVLLDKKFG
                     APTVTKDGVSVAK"
BASE COUNT          626 a         1245 c         1261 g          675 t
ORIGIN      
        1 ggcgaggctt cttctggttc ttcttcgagc gcaacgactc gccgaacctc gtggtcgagg
       61 actcgaaatt cccgatgcaa cgactatcgt ccggacggaa aggcgtctac ctgtaccgcg
      121 tgcgcgccca cgccgaccgc ctgtcgctcg agatgtgcca tatcctgacc gacggctacg
      181 gagcgatgat ctatttcagg tctctcctgg ccgagtactt caggctcaag ggcgttacga
      241 ccgagtacgg gcccatggtg ttcgccccga acgacgcgcc ggggcctgaa gaatgggagg
      301 acgcgttctc ccgctacgcg acgcccgggg cgccgggccc cgccaagcta ggtccggcct
      361 ggcacctgcc gggcataggc ctgcccgtcc atacgatgcg cgtcgtcacg ggagagctgt
      421 cgctgtcatc ggccctggcg aaggccaagg aacacggcgc caccctgacg atctacctat
      481 cgtcggtgtt cctcgccgcg ctgcaggacg tccaggacgc cgagtacgaa gggcggccgc
      541 ggtcgcgcag gcggcccctg cgcctccagg taccggccaa cctgaggaac ttcttcccct
      601 cgtcgaccct gcgcaacttc agcctctacg cgctgccgga gctggacacg aggctcggcc
      661 attacgaatt ctccgagatc gtcgcgaggg tcaaggcgac gatggcgctc gccttcacgc
      721 ccaaggagat catgcggacg atcacgcgga atgtggcgac ggccaaacat cccgtcatcc
      781 gggccgtgcc gttgacgatg aagaacctga tcatgcgcgg cctctaccgc agccacggcg
      841 aggccctcta ctcgagcctg tcgaccaacg tcggccaggc caggctaccc gactccttcg
      901 cgtccaaggt caaacgcatc gacgtggtgc tgccgtcgac tccgggcctc aagacggtcg
      961 ccggcatgct cagccacggc gacgcgctgt cgatcagctt cggtagcgtg atcgagcgac
     1021 gcgacctcga gagggccttt ttcacgcggc tcgtcaggga cgggctcaag gtacgggtcg
     1081 agtcgaacct tggcgcgccc gaaggaaagg acgcatagat gccttattgc agtagatgcg
     1141 gcgtcgaggt cgacgacgcg acgagcgatt gcccgctctg cgaggcgccg atccagcgcc
     1201 tggatgcgcc ggcttcaccg tcgggcgccg gtccctaccc ccagcacatc atcgacccgc
     1261 aggacaggta ccggctgtcc agggccgagc gtcggcgcat cgcgatcgag atactcagcc
     1321 tggtggccat actcggtagc ggagccctgc tgctgatgga tctcctatcg agcgccgacc
     1381 tcggctggtc ccgttacgcg gtggcttcca tcgtcttcgg ttggtgcgcc gcctgcctgc
     1441 cgatagcgct ctacggtagc cccaaggcgg cgatcgccgc cgtcggggcc gcgtcggtgg
     1501 ccttcctcgt cgcgctggac accatggacg gggcgatagg ctggtcattg tcgctcgggg
     1561 cgcccatagc gctcgccagc ttcgcggcgg tatcggccac ggcggcgtgc atagctaccc
     1621 gccgggtcaa ggggctcaac ctgcttggga taggggcgat cgggctggcc gcctacctcg
     1681 tcgcgctcga ggccctgctc aggataggcc tgctcggcct cgagcgcttc ggggaggtac
     1741 ggccgtattg gtcgctcgtc gcggcgctcg cgctcgtgcc ggtagcggtg ttcctgttct
     1801 acctgcacgg gcgggtggcg cgcggcgccg acctcaggaa aattttccgg ctctgacggc
     1861 tactgggcgg ccgcttcgat gccgagcagc tcgacctcga agaccagcgt cttgttcggc
     1921 tcgatgccgc cctgcgaacc ctgctcgccg taggccaggg ccgacgggat gtagaacttg
     1981 tacttggaac cgacgggcat cagctggagt ccctcgaccc aaccgggtat gaccatcgcc
     2041 accgggaaca cgaccggctc gccggcgttt ttcgagctat cgaattcctt gccgtcgagg
     2101 aaggtgccga cgtagtcgac gcggacggtg tcggactcgg ccggcttggg gccggaaccg
     2161 agcttgatca cctcgtactg gagcccgctg gcggtcgtcg taacgccgga cttcttgccg
     2221 ttctcggcga ggaaggcggc ctcggcttcg gcgttcctcg aggcgatagc ctgcatcgcc
     2281 tgcatgatgg ccgactggat ggtcatctgg gccgtttcca tcgacatgcg cggctctcgg
     2341 tcctcgatat agtccttcat gcccttcagg aactcgtcat agtcgagctc gacgccggta
     2401 tccttgaggc tggtgccgat ggcgaggccg aaggcgtagc tggcgtcggc cgctgaggcg
     2461 gcggcgtctt tggccgagtc ggccttatcg gcgcaggaga cgagcgagaa agataaggcc
     2521 gcgatagcgg cgaacgctac gatagtcttc ttcatggtac ttccggaaac ctactggata
     2581 gggacgccat gcgcccaccc cccgacgggg gtcggattaa gatagccgca agtcgcgata
     2641 aggacaaggc ggctcggcct gagcccgggg tcggctctag agcttgcgta gctgcttgac
     2701 cagggcgccg aagtccttgg ggtacggggc ctcgactcgc acggcctcgc cggtcaacgg
     2761 gtgcgcgaac gcgatcgaga aggcgtggag cgccgtcctc gatatcagcg gccgctcccg
     2821 gtcctcgtcg cccttccagc gtcgcttgat cttggacagg aagaccggct tgccgtcgcc
     2881 atagaggggg tcacaggcga ccgggtagcc gagcgcggcc aggtggacgc gtacctgatg
     2941 ggcccggccc gtcagcggcc gggcctcgac gatggccatc ttggcgtggc agccgagcac
     3001 cgcgaagtcg gtgagcgcgg gcttgcccga gccgtctatt accgtgcggt gcatcctgtc
     3061 gccgtcggcg gtcagcgaca ggtcgcagga gctatcgctc caggtcggcc gcccggccac
     3121 tacggcatgg tagatcgtcg tcacctcgcg gtcctcgagc ctggcgcaga ggaggcgcca
     3181 accggcctcg tcccgagcca ggagctggac gccgctcgcg tcgcggtcga gccggttgac
     3241 cggccagagg cggccgtatt ccatctcgag ctcgcgggcc gccacccggg cgtccggttc
     3301 gtagcggtcg ggaaaggaca ggatacctgc gggtttgctg atgacgagga tggactcgtc
     3361 catgaatacg atggggattt cttcgttcat acgcctcgat tatagcggaa cggaccgatt
     3421 cttcatagcg ccgcccccgc catccgctgt tttggtagct cttcgcccct tgccgcctgg
     3481 catcgttttt agtatcttcg ctatcgcgaa tcgtacgggt tcgcttcatt ctacgtacgc
     3541 caacccgccc aggaaacggc gctcgcgagc gcgctatcga gcgcgaggcc aagagcgggt
     3601 tggaccaccc acgagagaaa gggagataca agaacatggc gaagcaattg atgttcagcg
     3661 aggacgcccg gaagaagctc ctgtccggag tcgagcagat ttcgcgcgcg gtgaaggtaa
     3721 ccctcggccc caagggacgc aacgtcctcc tcgacaagaa gttcggcgcc ccgaccgtca
     3781 cgaaggacgg cgtatcggta gccaagg
//
LOCUS       DAOPHV010000221         3773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_33294_length_3773_cov_3.428994, whole genome shotgun sequence.
ACCESSION   DAOPHV010000221 DAOPHV010000000
VERSION     DAOPHV010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33294_length_3773_cov_3.428994"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="PLQ29_06895"
     CDS             complement(<1..1318)
                     /locus_tag="PLQ29_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG86411.1"
                     /translation="MAKTSYATLFDTALEAGARRDYAEAETTLTRIVAETDAMPEAWL
                     YLGRARYALGDYGRAIPAFSTFLGYDPESAAGWFFLGRGYLAIGRNRAAAYSLQRAID
                     KGRAGADAWALLGFAELRQKRSAKAVACLERAISLAPNDHRVYRAYLNALYVHAIRSL
                     ARGQAAEAASMLGFVIDNGLDGPAQRLYRSRALRASGDVPSAVAELELAVRAEPDDPS
                     LRLQLAALRFSAGDPDGAMAEIERSGVSLPEQRGAPWTADAVERWRVIVALRDGDYRT
                     ALKAALERIRRGDTDAAIRAVAAQANMELGRYDRAAAHYQRAVEADPGAPDLRIGLAL
                     AKYELGDYQAARAAARGATARGAARDDSLYVELLCDVKTGVDPRASLPTAQALLRARP
                     GDPRLMMALAECLYKTGEAALADGWFADVLRLWPSHELAMLYRVSVA"
     gene            1398..2735
                     /locus_tag="PLQ29_06900"
     CDS             1398..2735
                     /locus_tag="PLQ29_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPG86412.1"
                     /translation="MRDQTRKRLGRLAFPLGAGVLMLALAASLWALTLRERANRRLYL
                     EYEVHRVVTAMTDLVRLRTLEAGDVQDVVGFGLYAADGSAVSLYGEAPASMNVTTMGY
                     APSSFTLRESSVVMIRSIGGDLLGRRNMMGPERMATDRAARWRDIMQGRQGSAGALPP
                     PAEPPESPQAGSFARTGRQPAISYIEFSTQGFLAEQRLFLVIAVAATAAIAGLYLMLL
                     AMNRGYVEAKDREVRDRELVELGQAARTIAHEIKNPLGVIRIQCGLLRRGAPEGIVAG
                     LAIIDDEALRLAALADRIRSFLKSGEAKPERLDALGYLTDFGARYNGALELAVDVGAG
                     TSVLVDAARLTEALDNLVSNAIDASRGAAEPPRLEAMVRSRRLAIAVLDRGGGVPADR
                     ADRIFEPFFTTKERGSGLGLALARKNVEACGGRIAYADRPGGGAAFTVSLPIA"
     assembly_gap    2808..2907
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2908..3699)
                     /locus_tag="PLQ29_06905"
     CDS             complement(<2908..3699)
                     /locus_tag="PLQ29_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HPG86413.1"
                     /translation="MKSPKETSLERKLLEAARGEALRAAELLIADEEAQALQEYANVV
                     SIRRLNFNDHGPVHMRTVAINALRMLDLLQAAAVKTSLETNGAGSIEDSRVAVLMAAF
                     LHDVGMSIGRQNHEMNSAILAAPIIDRILQVMYPGETMRRSAIKCLALEGIEGHMATM
                     RIHSVEAGLILVADGCDMEKGRSRIPLMLSVEPRVGDIHQYSAAAISKVTIGPGEHRP
                     IRIDVSMEESVGFFQVEEVLLQKINMSPVKPMIELYAAVSGQPPRR"
BASE COUNT          586 a         1212 c         1277 g          598 t
ORIGIN      
        1 cggcgacgga cacgcggtac agcatggcca gctcgtgcga tggccagagc cgtagcacgt
       61 cggcgaacca gccatcggcc agggctgcct ctcccgtctt gtacaggcac tcggccagcg
      121 ccatcatgag cctcgggtct ccgggacggg cgcgtaacag ggcttgcgcc gtcggtagcg
      181 aggcgcgggg atccacgccg gtctttacgt cgcagagcaa ttcgacgtag aggctgtcgt
      241 cgcgggcggc cccgcgcgcc gtcgcgcccc tcgcggcggc cctcgcggct tggtaatcgc
      301 ccagctcgta cttggccaga gccagcccga tccggaggtc gggcgcgccg gggtcggcct
      361 cgacggcgcg ttggtaatgg gccgcggcgc ggtcatagcg gccaagttcc atgttcgcct
      421 gcgcggcgac ggctcggatg gccgcgtccg tgtcgccgcg cctgatgcgc tcgagcgccg
      481 ccttcagtgc ggtcctgtag tcgccgtcgc gtagcgcgac gatcacccgc cagcgctcga
      541 cagcgtcggc cgtccacggc gcgccacgct gctcggggag ggaaacgccc gagcgctcga
      601 tctcggccat cgcgccatcc gggtctcccg ccgagaaacg cagggccgcg agctgcagcc
      661 tgagcgacgg gtcgtcgggc tcggcgcgca ccgccagctc gagctcggcg acggcagacg
      721 gcacgtcgcc ggaggccctg agcgccctcg acctgtagag tcgttgagcc ggcccgtcga
      781 gcccattgtc gatcacgaag cccagcatcg aggcggcttc ggcggcctgg cctcgagcca
      841 gcgagcggat ggcgtgcacg tagagcgcgt tgaggtaggc tcggtagacg cgatggtcgt
      901 tcggcgccag gctgatagcc ctctcgaggc aggcgacggc cttggccgag cgtttctgtc
      961 gcagttcggc gaaaccgagc agcgcccagg cgtcggctcc ggcgcgcccc ttgtcgatgg
     1021 cacgttggag gctataggca gcggcgcggt tacggccaat ggccaggtac ccgcgtccca
     1081 ggaagaacca accggcggcg ctttcgggat cgtagccgag gaaggtagag aaggcgggaa
     1141 tggcgcgccc gtagtcgccg agcgcgtacc tggccctgcc caggtagagc caggcctctg
     1201 gcatggcgtc agtctcggcg actatcctcg tcagcgtagt ctccgcctcg gcgtagtccc
     1261 tgcgggcgcc ggcttcaagg gccgtatcga atagggtcgc gtaggaagtc ttggccatga
     1321 ggatactgta caggagaggc ggccaatcgt acaggggagg gcgttgcctc gagggccggg
     1381 gcggcgtaca atgcggtatg cgcgatcaaa ccaggaagcg cctcgggcgg ctggcctttc
     1441 cgctcggcgc cggcgtgctg atgctggcgc tcgcggcgtc gctctgggcg ctcacgctca
     1501 gggaacgggc caatcgtcgc ctgtacctcg agtacgaggt ccacagggtc gtcaccgcga
     1561 tgaccgacct cgtcaggctc aggacactcg aggccggcga cgtccaggac gtggtcggct
     1621 tcggtctata cgccgcggac ggtagcgccg tctcgctcta cggtgaggcc cccgcgtcga
     1681 tgaacgtcac gaccatgggc tacgcccctt cgagcttcac tctgcgggag tcgtcggtcg
     1741 tcatgatacg atcgatcggc ggagatctgt tgggccggag gaatatgatg ggtcccgagc
     1801 gaatggcaac ggatcgcgcg gcccgatgga gagatatcat gcaggggcgg caaggctcgg
     1861 ccggcgcgct accgccgccg gccgagccgc ccgaaagccc ccaggccggg tccttcgcgc
     1921 ggactggccg ccagccagcg atatcctaca tcgaattctc cacgcaggga ttcctagccg
     1981 agcagcggct attcctagtg atcgcggtcg ccgcgaccgc ggccatcgcg ggactatacc
     2041 tgatgcttct agccatgaac cggggctacg tcgaggccaa ggacagggag gtacgcgatc
     2101 gcgagctcgt cgaactcggg caggccgcca ggactatcgc ccacgagatc aagaacccgc
     2161 tcggtgtcat acgcatccag tgcggcctct tgaggcgcgg agcccccgag gggatcgtag
     2221 ccggtctggc catcatcgac gacgaggcct tacggttggc cgcgttagcc gaccggatac
     2281 gaagcttcct caagtcgggc gaagcgaagc ccgagaggct cgacgcgctc gggtatctga
     2341 ccgacttcgg ggcccgctat aacggcgcac tcgagctggc cgtcgacgtc ggcgccggga
     2401 cctcggtcct ggtggacgcg gccaggctga ccgaggccct ggacaacctg gtgtcgaacg
     2461 ccatcgacgc cagccgaggg gccgcggaac cgcctcggct cgaggccatg gtcaggtcgc
     2521 gacgcctggc gatagcggta ctcgaccgtg gcggcggcgt gccggcggat cgggccgata
     2581 ggatcttcga gccgttcttc accaccaagg aacgcggtag cggcctcggt ctggccctgg
     2641 ccaggaagaa cgtcgaggcc tgcggggggc ggatagccta cgccgaccgg cccggcggcg
     2701 gcgccgcctt caccgtgagc ctgccgatcg cctaagccag gtagcgccta ccccaggtag
     2761 cgcctacccc aggtagcgcc taccccaggt agcgcctacc ccaggtannn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnngcg cctcggcggc tggcctgaca cggcagcgta
     2941 cagctcgatc atcggcttga ccgggctcat gttgatcttc tggagcagga cctcttcgac
     3001 ctggaagaag ccgaccgact cctccatgct cacgtcgatg cgtatcggcc gatgctcgcc
     3061 gggacctatc gtcaccttgg agatcgccgc ggcggagtat tggtgaatat cgccgaccct
     3121 gggttccacg gacagcatca acgggatgcg cgaccgtcct ttctccatgt cgcagccgtc
     3181 ggcgacgagt atcaggccgg cttcgacgga gtggatgcgc atcgtagcca tgtggccctc
     3241 gatgccctcg agcgccaggc acttgatggc cgagcgccgc atcgtctccc cggggtacat
     3301 cacctggagg atccgatcga tgatgggtgc tgcgaggatg gcgctattca tctcgtggtt
     3361 ctggcggcct atcgacatcc ccacgtcgtg caggaaagcg gccatcagta ccgccacgcg
     3421 gctgtcctcg atggagccgg cgccgttggt ctcgaggctg gtcttgacag cggccgcctg
     3481 gagcaggtcg agcatgcgga gcgcattgat cgcgaccgtg cgcatatgca cgggaccgtg
     3541 gtcgttgaag ttgaggcgcc gtatcgacac cacgttggcg tactcctgga gggcttgcgc
     3601 ttcctcgtcg gcgatgagca gctcggcggc gcgtagcgcc tcgcctcgcg cggcctcgag
     3661 gagcttgcgc tcgaggctcg tttccttggg gcttttcata cgtatgaata tacagcattc
     3721 ggctcgcgaa gcgacggcgc ttcgcgacga tataccgaaa aaaagcgccc cgg
//
LOCUS       DAOPHV010000222         3743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_33665_length_3743_cov_3.989696, whole genome shotgun sequence.
ACCESSION   DAOPHV010000222 DAOPHV010000000
VERSION     DAOPHV010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33665_length_3743_cov_3.989696"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /locus_tag="PLQ29_06910"
     CDS             complement(<1..1445)
                     /locus_tag="PLQ29_06910"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPG86414.1"
                     /translation="MSKRLIELVPAGPPAELTGNPDVAIEGLAYDSREVGPGYLFFAF
                     PGLHADGHDYVAKAISAGAAAVVLESMPPSPAPGVSYLRVPDARAAMSPIADAFFGRP
                     SRRLKVIGVTGTEGKSTTVWLIYRLLDLAGKKAGFFSTVEYRVADRVEANPKHQTTPE
                     APTVHEHLARMAASGFEYAVVEASSHGLSPRTGRLADLAFDVGVMTNVRHEHLEFHGT
                     WERYRDDKMRLFQGLDSVDHRKSVLGVTVDVPSFGVVCADDPSAGRFAASTGKPVYRY
                     SASDPGADLYADDIRCDGLGASFIIREGTASYPARINLPGAFNVENALGATLVVSRLA
                     GVPVAELAPLLPALEPVRGRMTRVDRGQGFEVLVDYAHTPSSFEAILPPLAERVPGRL
                     ICVFGSGGERDVRKRPAQGRVASRYCDVVVLADEDPRGEDPMTLLEDIASGCEGLERG
                     VTLLLIPDRGTAIRKAIALARPGDAVLLLGKG"
     gene            1596..1955
                     /gene="queD"
                     /locus_tag="PLQ29_06915"
     CDS             1596..1955
                     /gene="queD"
                     /locus_tag="PLQ29_06915"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HPG86415.1"
                     /translation="MYTIRVEADFAAAHYLSEYHGKCERLHGHNYTVRAYAKGAELGE
                     GGMLVDFGALKGRLREVCASLDHSNLNDEPAFAASPSAERIAKYIFDGIAALEPGLPL
                     CAVEVFETDTSMARYEP"
     gene            1945..3654
                     /locus_tag="PLQ29_06920"
     CDS             1945..3654
                     /locus_tag="PLQ29_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HPG86416.1"
                     /translation="MSHRPVGRDAAALPGPPGYCSAQLIPYLGNKRALLPRLYPVFKR
                     LTSGRVGPLSFADAFAGSGAVSRLARAMGLRVASNDWEPYSQAIGHCWLALSPTDLDA
                     AFGGGQGLASFLDDWNSMHPMSGVPYGGAAEPYIARWYAPERTDAPRLGEERLFYTAE
                     NATFIDRVRERVESEYPSPEPLSPGDVRRRVAMGALLLEAAVHTNTSGVFKAYHRGFG
                     GHGKDALGRILGRMELEAPVLPDAPPAEVYGLDAAAFMAGKSFDIAYFDPPYNQHQYG
                     PNYHLLNTILVWDREPRPLASSERHSTKAGIPVDWKERRSPFCVRREAAGAIRSLLDA
                     TDAGSIVFSWNADGHLSGEDMAALLAPRGRLEIIALDYIAYRGGRQSASRSERSREYL
                     FVVDTRAEATDEDLARRSLADLAAVDEALRSSYDPERVRSVFFGSEAPWLEAAAFFGG
                     GMRRAADDAGQLLRAMPDDRRGPFCEALAACACSSVSDDLDALLATGNAAIGAGDRRA
                     ARIAGSKALRYVRKLAHEKYADEFRRYLGAFAAMAEACGDEATSVGLVELAQLVERRK
                     HGD"
BASE COUNT          590 a         1264 c         1289 g          600 t
ORIGIN      
        1 cccttgccga ggagcagtac cgcgtcgccg gggcgggcga gggctatcgc cttgcgtatg
       61 gccgtcccgc ggtcgggtat caacagtagc gtgacgcccc gctcgaggcc ctcgcagccc
      121 gaggcgatgt cttcgagcag ggtcatcggg tcttcgccgc gcgggtcctc gtcggccagc
      181 acgaccacgt cgcagtagcg cgacgcgacg cgtccctgag ccgggcgttt ccgtacgtcg
      241 cgctctccgc cggagccgaa cacgcagatg agtcggccag gtacccgctc ggcgagcggc
      301 ggcaggatgg cctcgaacga cgacggcgta tgagcatagt cgaccagtac ctcgaagcct
      361 tggcctcggt ctacgcgggt catacggccc cgtacgggct cgagggccgg caggagcggc
      421 gcgagctcgg ctaccgggac gccggccagg cgcgatacga cgagcgtggc accgagggcg
      481 ttctccacgt tgaacgcgcc gggcagattt atccgggccg ggtaggaggc ggtcccttcc
      541 cgaatgatga aggaagcgcc caggccatca cagcgtatat cgtcggcgta caggtcggcg
      601 cccgggtccg aggccgaata gcggtagaca ggctttccgg tcgacgccgc gaagcgcccg
      661 gcgctcgggt cgtcggcgca cacgacgccg aagctcggca cgtcaacggt gacgccaagc
      721 acggacttgc ggtggtcgac cgagtcgagg ccctggaaca gccgcatctt gtcgtcgcgg
      781 tagcgttccc aggtgccgtg gaactccaag tgctcgtggc gcacgttcgt catcacgccg
      841 acgtcgaagg ccaggtccgc gaggcgcccg gttcgcggcg acaggccgtg cgacgaggcc
      901 tcgacgacgg cgtactcgaa gcccgacgcg gccatgcgcg ccaggtgctc gtggacggtc
      961 ggggcctcgg gcgtagtctg gtgtttgggg ttggcctcga cccggtcggc cacgcggtac
     1021 tccacggtgg agaagaaccc ggccttcttc ccggccaggt ccaggagtcg gtagatcagc
     1081 cagaccgtcg tactcttgcc ctcggtaccg gtcactccga tcaccttgag ccgccgcgac
     1141 gggcggccga agaatgcgtc ggcgataggt gacatggcgg ccctcgcgtc gggcacccgt
     1201 aggtatgaca cgccaggcgc cggcgacggc ggcatgctct ccaggacgac cgcggcggcg
     1261 ccggccgaga tcgccttagc gacgtagtcg tggccgtcgg cgtggaggcc ggggaacgcg
     1321 aagaagaggt agccgggccc gacctcccgc gagtcgtacg cgaggccctc aatggcgacg
     1381 tcgggattcc ccgtcaactc ggcgggcggg ccagccggga cgagctcgat caatcgtttg
     1441 ctcatgccca cgatcctagc gacgccgctt ggcttgcgca atatatcgag cctcgtttac
     1501 ggcgaccatg ccgtaaaatt gccaggatcg gactcgggcg acgaacgctt gaaggctgaa
     1561 ccggcgctcg gtattattgt ggaactggag ccatcatgta cacgatacgc gtcgaggcgg
     1621 acttcgctgc cgcccattat ctgagcgagt accacggcaa atgcgagcgc ctgcacggtc
     1681 ataactatac ggtcagggcc tatgccaagg gcgcggagct gggcgagggc ggcatgctcg
     1741 tcgatttcgg cgcgctcaag ggcaggttgc gcgaggtctg cgcctcgctc gaccactcga
     1801 acctgaacga cgagcccgcc ttcgccgcgt cccctagcgc cgagaggata gccaaatata
     1861 tcttcgacgg catcgccgca ctggaacccg gcctgcccct ctgcgccgtg gaggtgttcg
     1921 agaccgatac cagcatggcg cggtatgagc cataggccgg tgggccgcga cgccgccgcg
     1981 ctgcccggac cgcccggcta ctgctcggcg cagctgatac cgtacctcgg caacaagcgc
     2041 gccctcctcc cgcggctgta cccggtcttc aagcggctga cctctggccg ggtggggccg
     2101 ttgtcgttcg ccgacgcgtt cgcaggtagc ggggcggtct cgaggctagc gagggccatg
     2161 ggcctcaggg tggcctccaa cgactgggag ccgtacagcc aggccatagg ccattgctgg
     2221 ctagccctga gcccgaccga cctggacgcg gccttcggcg gcggccaggg cctcgcctcg
     2281 ttcctggacg actggaactc gatgcacccc atgtcgggcg taccgtacgg aggcgcggcc
     2341 gagccgtaca tagcgcgctg gtatgccccg gaacgcaccg acgcgccgag gctgggagaa
     2401 gagcggctct tctataccgc ggagaacgcc acgttcatag accgcgtccg cgagcgcgtc
     2461 gagtctgagt acccatcgcc ggagcctcta tcgccaggcg atgtccgccg ccgtgtcgcg
     2521 atgggcgccc tcctcctcga ggccgccgtg catacgaata cctccggcgt cttcaaggcc
     2581 taccatcgcg ggttcggtgg ccacggcaag gacgccctcg ggcgtatact cggacgcatg
     2641 gagctcgagg cccccgtgtt gcccgacgcc cctccggccg aggtctacgg gctcgacgcc
     2701 gcggccttca tggcgggaaa gagtttcgat atcgcctatt tcgacccgcc gtacaaccag
     2761 caccagtacg gtccgaatta ccacctgctc aacaccatac tcgtctggga ccgcgagcct
     2821 cggcccttgg cctcgtcgga acgtcattct accaaggcag gcatcccggt cgattggaag
     2881 gagcggcgct cgccgttctg cgtacgacgc gaggcggccg gcgcaatacg atccctgctc
     2941 gatgccaccg acgccggctc catcgtcttc agctggaatg ctgacggcca cctgagcggc
     3001 gaggatatgg cggcccttct agccccacgc ggccgactgg agatcatcgc cctcgactac
     3061 attgcgtatc gcggcgggcg acagtcggcg tccaggagcg agcggagccg cgagtacctg
     3121 ttcgtcgtcg atacgcgcgc cgaggccacc gatgaggacc tggctaggcg gtcgctagcc
     3181 gacctggcgg ctgtggacga ggccttgcgg tctagctatg accccgagcg cgtccgctcc
     3241 gtgttcttcg gatcggaggc gccctggctc gaggcggccg cgttcttcgg gggcggcatg
     3301 aggcgggcgg ccgacgacgc cggccagctt ctgcgcgcca tgcctgacga taggcgcggg
     3361 ccgttctgcg aggcgctggc cgcttgcgcc tgctcctcgg tctccgacga cctggacgcc
     3421 ttgctcgcga ccggtaacgc ggctatcggc gccggagacc gtcgcgcggc caggatcgcc
     3481 gggtcgaagg ccctgcgtta cgtgcgtaag ctggctcatg aaaagtacgc cgacgaattc
     3541 cgacgatacc tcggcgcgtt tgcggcgatg gcggaggcct gcggcgacga agcgacgtcg
     3601 gtcggcctcg tcgaactcgc ccagctcgtc gagcgccgga agcacggcga ctgaaccggg
     3661 tagcgctatt cggcgctcgg cttctgagcg tccttggggc cgcccttgcg gcgacgatgc
     3721 ttacgcttgg cgggctggtt ccc
//
LOCUS       DAOPHV010000223         3737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_33745_length_3737_cov_3.499457, whole genome shotgun sequence.
ACCESSION   DAOPHV010000223 DAOPHV010000000
VERSION     DAOPHV010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33745_length_3737_cov_3.499457"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="PLQ29_06925"
     CDS             complement(<1..1321)
                     /locus_tag="PLQ29_06925"
                     /inference="COORDINATES: protein motif:HMM:NF014666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdrA family protein"
                     /protein_id="HPG86417.1"
                     /translation="MKRITVKRGAYYDSVFLMLATRGLKGLAGVRDAIVAMATPMNVE
                     LLCDMGFPSAELDGLGPNDLVVAVDAQNDQAAEAALKEAFDSLSKKRNAEVAGGVAKV
                     GSFAAALAHDPDANVAVISVPGAYAAREARKALQAGLHTMVFSDNVSLDDEIALKKLA
                     VSKGLLMMGPDCGTAIIDGKPLCFANVVRRGPIGVVAASGTGLQETTCLIDRYGSGVS
                     QAIGTGGRDMKNAAVGGMTSLLAIEALSADPSTRAILVVGKPPAPDVAAKVVAALRAS
                     GKPAVAHFLGAPIPADEETIAYASDLEEAALLVTLLADGSNDPASARARIRDDSGNNH
                     YNRPGKAAIETIARAEAERLGASQRYLRGLYTGGTLCDEALFLAHAALGGVRSNNQTD
                     PAWAMADPQRSEGHVVIDLGDDFFTAGKPHPMIDPAARAERLEKDGSD"
     gene            complement(1348..2208)
                     /locus_tag="PLQ29_06930"
     CDS             complement(1348..2208)
                     /locus_tag="PLQ29_06930"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HPG86418.1"
                     /translation="MTVGAIGCLAPGAPWSAPLAWVATKALALTHGDGAAVAIVGNIR
                     DMDARAILIADGFGDFRAAAKASADAVVSVRLDDVSATLVVDGVDLLAVPTGGALRWD
                     PRPALAAAAAAIASAAELTAAADVIAAMLAEGPADEVSVHGDGPFAVAFARLRADEAF
                     PANIVGFGPGSTPAGDDWLAGYLTAGDLYGGGPGTSWPGLRAALAPRLGRTTPAGRAL
                     LSGALAGAPPAYLADLALAVTGLSGDGADDGLRLAVRAALGKGASSGRDALAGFLEAL
                     RHRPKSSDAL"
     gene            complement(2284..3543)
                     /locus_tag="PLQ29_06935"
     CDS             complement(2284..3543)
                     /locus_tag="PLQ29_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HPG86419.1"
                     /translation="MDKEKDLTGRNVVMGLQHTFVMFGATVLVPILTGLDVGVTLFAA
                     GIGTWLFHIVTKFKVPVFLGSSFAFIPGIAAVGASQGLPYALGGIVVAGLLYVVVAII
                     FKFVSYDNLHKILPPQVTGPMIILIGLILAPTAIASANGTNSPAIAEAIGVNGCWLVA
                     LFTFAVGIFVKVAFPKFGWKFMSNLPVLIALVTGYLLAIIIGIVDFSSVKEAAWIGIP
                     KFTLPKFSVEAITIMLPIAIVTMVEHFGDVLAIGNVVGKDFIKDPGIHRTLIGDGLAT
                     SLSALIGGPANTTYSENTGAVALTGVKNPIVMRIAACFAIVLAMIPKFTALIGTIPGP
                     VIGGISILLFGMISSIGIKNMVDARLNLGNSKLLIITATMLVLGLGGATFAVGNVELS
                     GLGLAAIFGIVLNLILRPKDVTKADCD"
BASE COUNT          611 a         1228 c         1285 g          613 t
ORIGIN      
        1 gatcggaacc gtctttctcg agccgctcgg cgcgtgcggc agggtcgatc atcgggtggg
       61 gcttgccggc tgtgaagaag tcgtcgccca agtcgatgac gacgtggccc tcggaccgct
      121 gcgggtcggc catcgcccac gccgggtcgg tctggttatt cgaccgtacg ccaccgagcg
      181 cggcgtgggc caggaacagg gcctcgtcgc agagcgtgcc gccggtatag aggccgcgca
      241 ggtagcgctg gctcgccccg agccgttcgg cttcggcgcg ggctatggtc tcgatggcgg
      301 ccttgcccgg acggttgtaa tgattgttgc ctgaatcgtc ccggatacgg gccctggccg
      361 acgctggatc gttcgatccg tcggctagca gggtcacgag cagggcggcc tcctccaggt
      421 cggacgcgta ggctatggtc tcctcgtccg ccggtatcgg agcgccgagg aaatgagcca
      481 cggccggctt gcccgaagcg cgtagcgccg cgacgacctt ggccgcgacg tccggggccg
      541 ggggcttgcc gacgaccagg atcgcgcggg tggacgggtc ggccgatagc gcttcgatgg
      601 ccagcaggct ggtcatgccg cccacggccg cattcttcat gtcgcggccg ccggtgccta
      661 tggcctggct gacgccggag ccgtagcggt ctatcaggca ggtcgtctcc tggagaccgg
      721 tgcccgacgc ggctaccacg ccgatggggc cgcgtctgac gacgttggcg aagcatagag
      781 gcttgccgtc gatgatcgcc gtgccgcagt ccgggcccat catcaggaga cctttcgata
      841 ccgctagctt cttgagtgct atctcgtcat cgagcgagac gttatcggag aagaccatcg
      901 tatgcaggcc ggcctggagc gctttcctgg cttccctggc ggcgtaggcg cctggtaccg
      961 atatcaccgc gacgttggcg tcggggtcat gagcgagggc ggccgcgaac gagcctacct
     1021 tggcgacgcc gccggctacc tcggcgttgc gcttcttcga cagcgagtcg aaggcttcct
     1081 tgagcgccgc ttcggccgcc tggtcgttct gtgcgtcgac agcgacgacg aggtcgttcg
     1141 gtccgaggcc gtccagctcc gccgagggga agcccatgtc gcagaggagc tcgacgttca
     1201 tcggcgtggc catcgcgacg atggcgtcgc gtacgcccgc gaggcccttg aggccccgcg
     1261 tcgccagcat aaggaacacg gagtcgtagt aggcgccgcg cttgacagta attcgtttca
     1321 tcgcatcacc gaatccttcc gttcttatca tagcgcatcg ctggatttag ggcgatgcct
     1381 tagagcctct aaaaagccgg ccaaggcgtc ccggcccgag ctggcgccct tgccgagagc
     1441 ggcccgtacg gccaggcgca acccgtcgtc ggccccgtcg ccggatagac cggtgaccgc
     1501 cagcgccagg tcggctagat aagccggggg cgctccggcc agggcgcccg agagcagcgc
     1561 cctgcccgcc ggcgtcgtac gcccgagccg cggagcgagc gcggcccgga gtcccggcca
     1621 ggatgtcccg gggccgccgc catagaggtc gccggcggtc aggtacccgg ctagccagtc
     1681 gtccccggcc ggcgtcgagc ccggcccgaa gccgacgatg ttcgccggga acgcctcgtc
     1741 ggcccgtagc ctggcgaagg cgaccgcgaa gggaccgtcg ccgtggacgc tcacctcgtc
     1801 ggctggcccc tcggcgagca tggcggctat gacgtcggcc gcggccgtca gttccgcggc
     1861 cgacgctata gccgcggccg cggccgccag ggcggggcgc gggtcccagc gtagagcgcc
     1921 gcccgtcgga acggccagca ggtcgacgcc gtcgaccacg agcgtcgcgg acacgtcgtc
     1981 gagccggaca gagacaacgg cgtcggctga ggccttggcg gcggccctga agtcgccgaa
     2041 gccgtccgct atcagtatcg cccgagcgtc catgtcgcgt atgttcccga ctatcgccac
     2101 ggcggcgccg tcgccgtgcg tcagggcgag agccttggtc gcgacccaag ccagcggcgc
     2161 cgaccagggc gcccctggcg cgaggcaacc tatagcgccc accgtcatcg tcacactcct
     2221 cgcttgaaga agagggccgc ccccggaagg ggcggcccgt atcatcgtcc ctctatggaa
     2281 tcgtcagtcg caatcagcct tcgtgacgtc cttaggcctg agtatcaggt tgaggacgat
     2341 gccgaagatc gcggcgaggc cgaggccgga tagctcgacg ttgccgacgg cgaacgtggc
     2401 gccgccgagg ccaagcacga gcatcgtggc cgtgatgatg aggagcttcg agttgcccag
     2461 gttgagcctg gcgtcgacca tgttcttgat gccgatcgac gagatcatgc cgaacagcaa
     2521 gatcgagata ccgccgatga ccgggcccgg gatggtgccg atcagcgcgg tgaacttcgg
     2581 gatcatcgcg agcacgatcg cgaagcaggc ggcgatgcgc atcacgatcg ggttcttgac
     2641 gccggtcagg gccacggcgc cggtattctc cgagtaggtc gtgttggccg gtccgccgat
     2701 cagggcggat agcgaggtcg cgaggccgtc gccgatcagc gtgcggtgga tgcccggatc
     2761 cttgatgaag tccttgccga ccacgttgcc gatcgcgagc acgtcgccga agtgctcgac
     2821 catcgtcacg atcgctatgg gtagcatgat cgtgatagcc tcgaccgaga acttcggcag
     2881 cgtgaacttc ggtatgccta tccaggcggc ttccttcacg gagctgaagt cgacgatgcc
     2941 gatgatgatc gcgagcaggt atccggttac cagagctatc agcacgggca ggttggacat
     3001 gaacttccag ccgaacttcg ggaacgccac cttgacgaag atgcccaccg cgaaggtgaa
     3061 cagtgccacc agccagcagc cgttgacgcc gatagcctcg gcgatcgccg gcgagttggt
     3121 gccgttggcg ctcgcgatcg cggtaggggc gagtataagc ccgatcagaa tgatcatcgg
     3181 gccggtgacc tgtggcggca gtatcttgtg caggttgtcg tacgagacga atttgaagat
     3241 gatcgcgacc accacgtaca ggaggccagc caccacgatg ccgccgagcg cgtagggcag
     3301 gccctggctg gcgccgaccg cggcgatgcc gggtatgaag gcgaaggagc taccgaggaa
     3361 caccggcacc ttgaatttgg tgacgatgtg gaacagccag gtaccgatac cggcggcgaa
     3421 cagcgtcacg cccacgtcga ggcccgtcaa gataggcacg agcacggtcg ccccgaacat
     3481 gacgaacgta tgctggagcc ccatcacgac gttcctgccg gtcagatctt tttctttatc
     3541 cacggcccac tccttccacg ggcggcgata ggggtcgtcc gcccgcgctc tatcggcggt
     3601 agcctcccca gcggccgacg gccgccttcc ggggggcccc gctacggtcg cccgtagggc
     3661 tgaccggtcg gtccctgtcc atcgcctcag ctcctcggcc tcgcttccgg cactgaacgg
     3721 cacgagccta tcggcga
//
LOCUS       DAOPHV010000224         3736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_33754_length_3736_cov_3.792176, whole genome shotgun sequence.
ACCESSION   DAOPHV010000224 DAOPHV010000000
VERSION     DAOPHV010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33754_length_3736_cov_3.792176"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PLQ29_06940"
     CDS             <1..681
                     /locus_tag="PLQ29_06940"
                     /inference="COORDINATES: protein motif:HMM:NF015970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HPG86420.1"
                     /translation="SDVDWPTAARDTIAADGYAATGATNLVSAIYLGYRAFDTLGETI
                     VLLVALAGSVALVASTPGRPDLDRDGGPADSRGPGHTDIINVIAGKLAPIVLLFGTYV
                     MLYGHTSPGGGFQGGVVLASGVIFIAIGRRAGGIGRISPSITAFGPRTLSVIEAIAFA
                     TVLALSFPGSGAALAGLIEATGASAGVAAKGVAAVGPIIALNIGIGLKVGSGVALLCV
                     MMLGGDQE"
     gene            678..1016
                     /locus_tag="PLQ29_06945"
     CDS             678..1016
                     /locus_tag="PLQ29_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HPG86421.1"
                     /translation="MIRRLLVLALFGTGLAGLVTKRNLIKKAYALAIMNSAIVILFVL
                     EGAAIGSDAPLLGGAGKLFVDPIPQALMLTAIVVGVCVSALALTLAYRLYRHFGTLDI
                     DEIKRRIGHE"
     gene            1009..2709
                     /locus_tag="PLQ29_06950"
     CDS             1009..2709
                     /locus_tag="PLQ29_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPG86422.1"
                     /translation="MSDSELAMLPVFLPLAGAALSLLAKALHGGAKARALEDSGAFIG
                     LALPWAALAAMLPRVLAGGVSFTVSGWSGAIGIAQRFDGLTWLVDVLGLTGAGAAYLY
                     SRGNGPKGPLFTTVYLIQASALAMTAATADLFNLFVCLEILGLASYVLVASSEKPGAF
                     LAAFSYLAVSSAAMVFFLFGLFGFYRVTGTLSYEGIQAALVALPDGGGAIATMSTACL
                     AAAIAIRVAVMPVYGWLPDAHALAPHAVSAILSGVLIKTPLFALGRLLGYLPSGQEAM
                     ALIGSAGVVTALAAVVVALSQKDAKRLLAYHSISQIGYVVAAWGLGAPAAMAAAFLHA
                     FYHALFKGLLFLSVGTITDAAGTRNVYELRGAAGTLGKSGDRFRLVGVAYAVGALSIM
                     ALPPLNGFASKNAIGYLFKGGWRYWALFAAGACTVASFLKLSMIFLPARRGAPADAAG
                     VAGSVPGLRIGGTMKASMLILASLCVATGLFADATTAAVSRLLGAPPPAGGAFTAANL
                     LKALQTLALGAAVFGLAMSKPGKRLAAAIRGRPRSFAGLVMAFLGGLAVLAAALARFG
                     "
     gene            complement(2781..2990)
                     /gene="rpsU"
                     /locus_tag="PLQ29_06955"
     CDS             complement(2781..2990)
                     /gene="rpsU"
                     /locus_tag="PLQ29_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPG86423.1"
                     /translation="MSQILVDDNEPLEKAIKRFKRMVEKEGIIREWKKREFFEKPSTI
                     RNRKEKAMRRKLLRKSRKPRDGKSY"
     gene            complement(2997..3521)
                     /locus_tag="PLQ29_06960"
     CDS             complement(2997..3521)
                     /locus_tag="PLQ29_06960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPG86424.1"
                     /translation="MTEQAIRYQALLSSVDPEAAFLDSGFSMPVATERRTTLFGMRRR
                     YVYADGGVEASTHNGIDYGLPTGSPIVAPGRGRVVMVEDRIVTGKTVILEHLPGTYSI
                     YMHLDGFDPSLALGAVVPRGSPLGTVGMTGLATGPHLHWELRVMGQACDPEALVGLDK
                     VPWIRTMMPAIEGR"
BASE COUNT          545 a         1257 c         1235 g          699 t
ORIGIN      
        1 tccgacgtcg attggccgac cgcggctcgg gataccatcg ccgccgacgg ctacgccgcc
       61 acaggcgcta cgaacctggt cagcgccatc tacctcgggt atcgcgcctt cgacacgctc
      121 ggggagacga tagtgctcct ggtcgccctg gccggctccg tggccctggt cgcctcgact
      181 ccaggccgtc ccgacctgga ccgggacggg gggccggccg actctcgcgg ccccggccac
      241 accgacatca tcaacgtcat cgccggcaag ctcgcgccca tagtgctcct cttcggcacc
      301 tacgtgatgc tctacggcca cacctcgccg ggtggcgggt tccaaggcgg cgtcgtactg
      361 gcgtccggag tcatcttcat tgccattgga aggcgagccg gcgggatagg caggatcagc
      421 ccgagcatca cggcgttcgg accgcgcacg ctgtccgtca tcgaggcgat agccttcgcg
      481 accgtcctgg cgctgtcgtt ccccggctcc ggcgcggccc ttgcaggcct gatcgaggct
      541 accggcgcat ccgcgggcgt cgccgctaaa ggcgtcgccg ccgtgggccc gatcatcgcc
      601 ctgaacatcg gcataggcct taaggttggt tccggcgtcg cgctgctgtg cgtgatgatg
      661 ctcgggggcg accaggaatg atacgtcgtt tattggtcct cgcgctgttc gggaccgggc
      721 tggccgggct cgtcaccaag cggaacctta ttaagaaggc ctatgccctg gcaataatga
      781 actcggccat cgtcatcctg ttcgtattgg aaggcgccgc gatcgggtcc gacgcgccgc
      841 tactcggcgg ggcgggaaag ctgttcgtcg atcccatacc ccaggcgctc atgctgacgg
      901 ccatcgtcgt cggcgtatgc gtatcggccc tggcgttgac gctggcgtat aggctgtacc
      961 gccatttcgg cacgctggat atcgacgaga tcaagaggcg catcggtcat gagtgactcc
     1021 gaactcgcga tgctcccggt attcctaccg ctcgccggag ccgccctgtc cttgctcgcc
     1081 aaggcgctac acggcggcgc gaaggcgcgg gcgctcgaag actccggcgc cttcatcggg
     1141 ctagcgctcc cctgggcggc gctcgccgcg atgctaccca gggtgctggc cggcggcgta
     1201 tcgtttaccg tatcgggctg gtccggcgcc atcggcatag cccagcgctt cgacggcctt
     1261 acctggctcg tcgacgtcct ggggttgacg ggcgcgggtg ccgcctacct gtactcgcgc
     1321 ggcaacggtc ccaaggggcc gctcttcacg accgtctacc tcatccaggc ctcggcgctc
     1381 gcgatgaccg ccgcgacggc cgacctcttc aatctgttcg tctgcctgga gatactcggg
     1441 ctggcgtcgt acgtgctggt cgccagctcc gagaagccag gtgccttcct ggcggcgttc
     1501 agctacctgg ccgtcagttc ggccgccatg gtcttcttct tattcgggct gttcggtttc
     1561 tatagggtga cgggtacctt atcgtacgag ggaatccaag cggctctcgt agccctgcca
     1621 gacggcggag gcgccatcgc gacgatgtcg accgcctgcc tggccgccgc gatcgcgata
     1681 cgcgtcgccg tcatgccggt ctacggctgg ttgcccgacg cccacgccct ggccccgcat
     1741 gcggtatcgg ccatcctgtc cggcgtactc atcaagacgc cgctgttcgc cctcggccgg
     1801 ttgctgggct acctgccgtc ggggcaggaa gcgatggcgc tgataggctc ggcgggcgtc
     1861 gtaacggcgc tggcggctgt cgtcgtcgcc ctatcgcaga aggatgccaa gcgcctactg
     1921 gcgtaccact ccatcagcca gatcgggtac gtggtggcgg cctggggcct cggagccccg
     1981 gcggccatgg cggccgcctt cctgcacgcc ttctaccacg cgctcttcaa gggcctcctc
     2041 ttcctgagcg tcggcaccat tacggacgcg gccggtaccc ggaacgtcta cgagctgcgt
     2101 ggcgccgccg ggacgctcgg caagtccggg gaccggttcc gcctcgtcgg cgtggcctac
     2161 gccgtcgggg ccctgtccat catggccctg ccgccgctca acggtttcgc cagcaagaac
     2221 gccatcggct acctgttcaa gggcggttgg cgatactggg cgctgttcgc cgcgggagcc
     2281 tgcaccgtgg cgtcgttcct gaagctctcg atgatcttct tgccggctag gcgaggcgcc
     2341 ccggccgatg ccgccggcgt tgccggctcc gtaccgggcc tcaggatcgg agggacgatg
     2401 aaggcgtcca tgctgatcct cgcctcattg tgcgtcgcga cgggtttatt cgcggacgcg
     2461 acgactgccg ccgtaagccg cctgctcggc gcgccaccgc cggcgggcgg cgcgttcacg
     2521 gcggcgaacc tgctcaaggc gctccagacc ctggcgctcg gagcggcagt attcggcctg
     2581 gccatgtcca aacccggcaa gcgtctggcg gcggccatcc gaggccggcc gcgtagcttc
     2641 gccggcctgg tcatggcgtt cctcggcggg ctcgccgtac tcgcagcggc tctggccagg
     2701 ttcggctagg cggctgggtt cgatcccaac atacaaaaga ccccggcata gccggggtcc
     2761 gtcgtgtacc gaatcagtcc ctagtaggac ttgccatcgc ggggcttgcg ggacttacga
     2821 aggagcttgc gcctcatggc cttctccttg cggttccgga tggtagaagg cttctcgaag
     2881 aactcgcgct tcttccactc gcggatgatg ccctctttct cgaccatgcg cttgaagcgc
     2941 ttgatggcct tctcgagcgg ctcgttatcg tcgactagta tctggctgat agaaaatcac
     3001 ctcccctcga tagcgggcat cattgtacgg atccacggca ctttgtcaag gccgacgagg
     3061 gcctccgggt cgcaggcctg gcccatgacg cgtagttccc agtgcagatg cggccccgtg
     3121 gccagtccgg tcatgccgac cgtaccgagc ggcgagcctc gcgggaccac cgccccgagc
     3181 gccaggctcg gatcgaagcc gtccagatgc atatatatac tgtaggttcc gggcaggtgc
     3241 tccaagatga cggtcttgcc ggtcacgata cggtcctcga ccatgacgac cctgccccgt
     3301 cccggggcga cgatggggct tcccgtcggc aggccgtaat cgatgccgtt atgcgtcgac
     3361 gcctcgacgc cgccgtccgc gtagacatag cgccgtctca tgccgaacaa agtggtacgt
     3421 cgttcagtgg ccacgggcat cgagaacccg gagtccagga aggccgcctc ggggtcgacc
     3481 gacgagagca gggcctggta gcggatggcc tgctcggtca tgttgttagt gggctcgacc
     3541 cctggggtgg taacgaaggt gaagaactcc tggggtggag tcggaagcgc tcgcgcaggt
     3601 cacagacgcc ggaacgttcc ggggaatgtt gaaagcgaag gattagccgg tcgcgcaggg
     3661 tcgagagcct gcgcgtgaag ttgtcggggt gcagttgttc gcgtgcggcg gccacgtcgg
     3721 gcgacaggtg ctccag
//
LOCUS       DAOPHV010000225         3731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_33813_length_3731_cov_4.020403, whole genome shotgun sequence.
ACCESSION   DAOPHV010000225 DAOPHV010000000
VERSION     DAOPHV010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33813_length_3731_cov_4.020403"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      159..289
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            281..1921
                     /gene="pruA"
                     /locus_tag="PLQ29_06965"
     CDS             281..1921
                     /gene="pruA"
                     /locus_tag="PLQ29_06965"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HPG86425.1"
                     /translation="MYDSLMYGSEPSPGLPPNEPVRSYAPGSLERSAIDAELKAMAAN
                     PIEIPLVIGGKEVRTGRIKEIRAPHDRSLVLGRYHEAGEAEAIAAAEAAAVAYREWSE
                     TPFQERAAVFRKAADLISAGWWKALNASTMLGQSKNAYQAEIDSTCETADFFRINPAL
                     AERLYAEQPRSGPGERNLSDYRPLDGFVYAVTPFNFTAIAANLPSAPALMGNTVVWKP
                     ASSSVLSNWQLLRLYKAAGLPDGVINFLPGSARAISAGVLSRPGLAGLHFTGSTAVFN
                     SLWKGIADNLSIYASYPRLVGETGGKDFIFVHPDADLDAAVTACVRGAFEYQGQKCSA
                     ASRLYLPRSIAGRFIERLAAEIASLRVGPPTDHANFVNAVIDEAAFDTIAGFIDRAKA
                     NPACRIAAGGGYDKSQGFFIDPTLIVSDDPRSETMANEVFGPVLTAYAYDDSDMAAAY
                     ELVDTTSPYALTGAVFASDRHAIMEATRALRHAAGNFYINDKPTGAVVGRQPFGGARG
                     SGTNDKAGSFLNLVRWTSPRTIKENFAPSVDWRYPFLG"
     gene            complement(1961..2905)
                     /locus_tag="PLQ29_06970"
     CDS             complement(1961..2905)
                     /locus_tag="PLQ29_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPG86426.1"
                     /translation="MAPKATGTMRIAAIEAGGTKFVLGLLEVASADVAPRILTKRRIP
                     TGRPEETIAEAERWFSSVFGEFGPADALGVASFGPLAGRFGESGWGRIGPTPKPGWAG
                     FDLPGALGAALGLEPAVDTDVNAAALAEGLWGASHGLRDHVYVTVGTGVGGGVVSNGA
                     LLHGAGHPETGHVPVPREPDDDYFGRCPFHGACLEGMASGPAVAERWGAPAESLPADH
                     PAWDLEARYLASGFLPLVHVLATERIVLGGGLGSAPGLAERVRSRLSERLAGYTPRLD
                     EPGAMEAFIVRPGLGGDAGLLGAAALTIGAAALTIAAG"
     gene            complement(2895..3371)
                     /locus_tag="PLQ29_06975"
     CDS             complement(2895..3371)
                     /locus_tag="PLQ29_06975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG86427.1"
                     /translation="MINIREYELLSLETGAYRDIELDILKETLATWKERPGNPFELIE
                     LRDGATLAGFCLYYRAPNTDYTFDVHTFVVGRDYRNKAVGPRLVELLEERLLESRSYA
                     VIRTETSKLKEDSIGDGFFQSIGFQTIGHIPAFYDADNDYYIYVKSVQADTKRDGA"
BASE COUNT          631 a         1258 c         1220 g          622 t
ORIGIN      
        1 tcaataagca gtatattgat atatcagtcg gtatacgaag ctggctcgtc ggtatggccg
       61 acgtaccgca tcgaaccgcc ccgccgtcgg cgggggcgcc gcggccagac atggcagccc
      121 gatggcgatt atcgccacgg cgagcgctga aggtctttta cagaaccatg cttcgagctc
      181 cgatagccta cagccgggca agccgcccgc gccacggccc ggagccgacg ggccgagtcc
      241 gaaagggatc gacctcccgc tatttggaaa ggagtatggc atgtacgatt ctcttatgta
      301 cggaagcgag ccctccccgg gcttgcctcc caacgagccg gtgcgttcgt acgctcccgg
      361 ttccctcgag cgctcggcca tcgacgccga gctcaaggcc atggccgcga atcctatcga
      421 gattcccctc gtcatcggcg gcaaggaagt ccgcacgggg cggatcaagg agatccgggc
      481 gccgcacgac cgctcgctcg tactcggccg ctaccacgag gccggcgagg ccgaggcgat
      541 cgcggcggcc gaggccgccg ccgtggccta ccgggagtgg tccgagacgc cgttccagga
      601 gcgggcggcg gtattccgca aggctgccga cctgatctcc gccggctggt ggaaggcgct
      661 caacgcgtcg acgatgcttg gccagtccaa gaacgcctac caagctgaga ttgactcgac
      721 ctgcgagacc gccgacttct tccgtatcaa cccggccctg gccgagcggc tctacgccga
      781 gcagccgcgc tccggtccgg gcgaacgcaa cctgagcgac taccggccgc tggacggctt
      841 cgtctacgcc gtcacgccgt tcaacttcac ggccatcgcc gcgaacctcc cgtcggcgcc
      901 ggccctgatg ggcaataccg tggtctggaa gccggcctcg agctcggtgc tgtccaattg
      961 gcaactcctg cgcctgtaca aggcggccgg gctgcccgac ggcgtgatca actttctgcc
     1021 gggctcggcc agggcgatca gcgccggggt gctgtccagg ccgggcctgg cggggcttca
     1081 ctttaccggc tccacggccg tgttcaacag cctctggaaa ggcatcgccg acaacctatc
     1141 catctacgcc tcgtacccgc gcctggtcgg cgagaccggc ggcaaggatt tcatcttcgt
     1201 tcatcccgac gccgacctcg acgcggccgt cacggcctgc gttcggggag ccttcgagta
     1261 ccagggccag aagtgctcag cggcctcgcg gctctacctg ccccgctcga tagccgggcg
     1321 ctttatcgaa cggctggcgg cggagatcgc ctcgctacgc gtcggcccgc cgaccgacca
     1381 cgcgaacttc gtcaacgccg tgatcgacga ggcggccttc gatacgatcg ccggtttcat
     1441 cgaccgcgcc aaggccaatc ccgcctgccg tatagccgcg ggcggcggct acgacaagag
     1501 ccagggcttc ttcatcgatc cgactctgat cgtcagcgac gacccgcgct ccgagacgat
     1561 ggccaacgag gtattcgggc cggtgctgac cgcgtacgcc tacgacgact ccgacatggc
     1621 cgccgcgtac gagctagtcg atacgacctc gccctacgcg ctgaccggcg cggtgttcgc
     1681 ctcggatagg cacgccatca tggaagcgac gcgcgcgctc aggcacgccg ccggcaactt
     1741 ctacatcaac gacaagccca cgggcgcggt cgtcggtagg cagcccttcg gaggcgctcg
     1801 tggctccgga acgaacgaca aggccggctc gttcctgaac ctggtccggt ggacgagccc
     1861 gcgcacgata aaggagaatt tcgcgccatc ggtcgactgg cgctatccgt tcctggggta
     1921 ggggaaaggg ccggccagcg tccggccccg ctttcggcgt ctatccggcg gcgatggtca
     1981 acgcggcggc gccgatggtc aacgcggcgg cgccgagcag gccggcgtcg ccgccgaggc
     2041 cgggcctcac gatgaacgct tccatcgctc ccggctcgtc caggcgcggc gtgtagccgg
     2101 ccaggcgctc ggatagcctc gagcgcaccc gctcggccag ccccggtgcg gagccgaggc
     2161 cgccgcccag tacgatacgc tcggttgcga gtacgtgcac gaggggtagg aagccggaag
     2221 ccaggtaacg cgcctcgagg tcccaggccg ggtgatccgc cggcagcgac tcggccggcg
     2281 cgccccagcg ctcggcgacc gccggtcccg aggccatgcc ctcgaggcag gcgccgtgga
     2341 aggggcagcg gccgaaatag tcgtcgtcgg gttcgcgcgg cacgggcacg tgcccggttt
     2401 ccgggtggcc cgcgccgtgg agcagggcgc cgttcgatac gacgccgccg ccgacgccgg
     2461 tgccgaccgt cacgtagacg tggtcgcgca ggccgtgcga ggcgccccag aggccctcgg
     2521 ccaaggccgc ggcgttcacg tcggtgtcca cggccggctc gagccccaag gccgcgccga
     2581 gggcgccggg caggtcgaag cccgcccagc ccggcttcgg cgtcgggcct atgcgccccc
     2641 agcccgattc gccgaacctg ccggcgagcg gcccgaacga agcgacgccg agcgcgtccg
     2701 ccggcccgaa ttctccgaag accgaggaga accagcgctc cgcctcggct atcgtctctt
     2761 cggggcggcc ggtcgggatc cggcgcttgg tcaggatcct gggcgcgacg tcggccgagg
     2821 ctacctcgag caggccaagc acgaatttgg tgccgccggc ctcgatagcg gctatgcgca
     2881 tcgtccccgt cgccttaggc gccatcgcgc ttcgtatcgg cctggaccga cttgacgtag
     2941 atgtagtagt cgttgtccgc atcgtagaac gccggtatat ggccgatggt ctggaagccg
     3001 atgctctgga agaacccgtc gccgatggag tcttccttga gcttgctcgt ctcggtgcgt
     3061 atgaccgcgt agctccttga ctcgagcagc cgttcctcga gcagttcaac gaggcgaggg
     3121 ccgacggcct tgttcctgta gtcgcgcccg acgacgaagg tgtgcacgtc gaaggtatag
     3181 tcggtgttcg gcgcgcggta gtacaggcag aagccggcca gcgtcgctcc gtcgcgcagc
     3241 tctatgagct cgaacgggtt gccgggcctc tctttccagg tggcaagcgt ttccttgagt
     3301 atatcgagct cgatgtcccg gtaggccccg gtctcgagcg atagcagttc gtattcacga
     3361 atgtttatca ttcacttctt ctccgagcga ttcgccgatg acggcgtttt tatactgcga
     3421 cacgaaccat accaggcgat tttaatctat aaagccttga ttgtatacaa tatcggcata
     3481 ttatacgtat accattcgtc gcgcgctcgc gatggacatt atcggtcaat gttttagggt
     3541 atcgggccga aatataataa gagcagaaaa cgaagtgtcg gtgcccgccg tatccgccgt
     3601 gacggatccg taggcggccg aggcaaggat ggaatgtagt gacggaatat actttatata
     3661 acgccgacat agcgagcggc gacgtcgtcc gcgccgcccg tccagcgcga gaagatgaac
     3721 ggcgcgcggt a
//
LOCUS       DAOPHV010000226         3726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_33864_length_3726_cov_3.935440, whole genome shotgun sequence.
ACCESSION   DAOPHV010000226 DAOPHV010000000
VERSION     DAOPHV010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33864_length_3726_cov_3.935440"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLQ29_06980"
     CDS             <1..518
                     /locus_tag="PLQ29_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86428.1"
                     /translation="GNSYDMTIIPGDGEVGRGFYEPIWTFMPIPFEISASVYEAIPDQ
                     AGLPGTAESRKATFAKFYEQSGAIWKLKKNRQPDYESRPSIWIMLEDARYNVKQAEYK
                     TITSLRPVVILFKVSAPGPDGTPTLQEVEIEGMGSGVSDIDNAVLYGFKKAAFRNSGS
                     SSISGRFTYRF"
     gene            566..2134
                     /locus_tag="PLQ29_06985"
     CDS             566..2134
                     /locus_tag="PLQ29_06985"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPG86429.1"
                     /translation="MVKTIVIAGIVAATLANLWLGAFSFLKARSRGRYYSLPALFAGG
                     ALYAACYVVELLRPDLDGVLACLGFEYLGIQTMMLSLVFLAREFKGKEGIRPLALLAL
                     ATIPVVTVVLGFTVSSHELLYVDPYVESIHGLTVVRFGMGPWYWVNVLFVYGVYAYAG
                     VEFARILIRSASMRKAQARLMLAGCAVPFAESILYLAGLTPGGIELSPVAFALSGAFL
                     ASGIFRYGLFDVRPIARDAVFEHMHDAAIVVDADGFIADANRTARGLFPRLDEDGSCL
                     PLSELGSAYPELARLPAPGTLADTASLRLRVGEEERAFDVQASNISGRSGRNRGSVIL
                     LYDVTERERLQACLIEQASTDDLTRVANRRRFYEVAEAELERAGRHGRPIGFAILDMN
                     GFKDINDGYGHQAGDDALRLVARLCVGELRSCDLVGRVGGDEFAFVLPESDEEGAAAA
                     AGKLRRLVGAATLTVGTATVRLSAAVGSVGCSGPVYPDLAELLTIADRRMYADKPRFA
                     AVKANKAAKTAAPR"
     gene            complement(2131..3399)
                     /locus_tag="PLQ29_06990"
     CDS             complement(2131..3399)
                     /locus_tag="PLQ29_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPG86430.1"
                     /translation="MIAFVIALGGLVGVTILIQLLTNMKIVGGNELGIVTGSGGDKGF
                     RTLSGGRAFIIPLLQRFSKFDLTPITIEVVVDSAIAAGIVPLNVKATVSFAIAGNDTG
                     RSHAVTRILDIASNRENLTKIAGDIIEGHLRDSIASMTPEQVMKDKDILVAKMINVCK
                     NDLENIGLEITTMNIADVDDHRLPGVEEPDLYIALLKRIQSANAETKARQAQAEARAA
                     SAEQAEARRAETEVKNLGNELTRLKAEVRVKLAEEAQRQKVGVEQESAGARARVSGYK
                     AELEAERQNIEMLKNRYRAEIVTPALAEKEKSILEARAEVSGWREVAEAELEQLAVTL
                     ETMKAAGGEARRAWVLDNFEKLFEPFAKTLALYPTDHVSVVTGSGGDREPISAIHPTA
                     VAAARNDIVAAALEGMTRPGAGLADLGKKG"
     gene            complement(3396..>3726)
                     /locus_tag="PLQ29_06995"
     CDS             complement(3396..>3726)
                     /locus_tag="PLQ29_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86431.1"
                     /translation="GERSAVSIRQTAHNEVLKAKAGLLAAYGDDASSVMFLQQKLPEL
                     YEAYMAATGSGAVDNLVVMNDEEGFSGAVNRGPRAFADFLRTFADAFGVDVRSLAMPA
                     ASGGVSK"
BASE COUNT          605 a         1240 c         1195 g          686 t
ORIGIN      
        1 gcggcaactc ctacgacatg acgataatac ctggagacgg cgaagtcggg cgcggcttct
       61 acgagcctat ctggacattc atgccaatac cattcgagat atcggcgtcg gtgtatgaag
      121 ccattccaga ccaagccgga ctacccggca cggccgagag tcgcaaagct acgttcgcca
      181 agttctacga gcagtccggc gcgatctgga agctaaagaa gaacaggcaa ccggactacg
      241 agtctcgtcc gtcgatctgg atcatgctgg aagacgcgcg atacaacgta aagcaagccg
      301 agtacaagac gattactagc ctccggcccg tcgtcatcct gttcaaggta tcggctccgg
      361 ggcctgacgg tacgccaacg cttcaagaag ttgaaataga aggtatgggc tccggcgttt
      421 cagatattga caacgcagtg ctctacggtt ttaagaaagc agcattccga aactcgggct
      481 catcgtcgat ctcggggcgg tttacctatc ggttctaggg gctatacgta aggtaccggg
      541 tcgcccggcg gaagagaagg cgctcatggt caagactata gtaatcgcgg gcatcgtggc
      601 cgcgacactc gccaacctct ggctcggcgc gttctccttc ctgaaggcgc gctccagggg
      661 caggtactac tccctgccgg cactgttcgc gggcggagcg ctctacgccg cctgctatgt
      721 cgtcgagctg ctccggcccg acctggacgg agtcctggcc tgcctaggct tcgagtacct
      781 gggcatacag accatgatgc tgtcgctcgt attcctggcc agggaattca agggcaagga
      841 agggatccgg ccgctcgcgc tcctggcgct ggctaccatc cccgtcgtca ccgtcgtcct
      901 gggcttcacc gtctcgtcgc acgagctcct atacgtcgat ccgtacgtcg agtcgataca
      961 cgggctcacc gtcgtacggt tcggcatggg cccgtggtac tgggtgaacg tgctcttcgt
     1021 ctacggcgtc tacgcgtacg ccggggtgga gtttgctcgc atactgatac gttccgcctc
     1081 gatgcgcaag gctcaagcca ggctcatgct ggcaggatgc gccgtgccgt tcgccgagtc
     1141 gatcctctac ctggccggcc tcacgcccgg cggcatcgag ctatcgccgg tcgccttcgc
     1201 gctcagcggc gcgttcctcg cctccggtat cttcaggtac gggctgttcg acgtgaggcc
     1261 gatagcccgg gacgctgtat tcgagcacat gcacgacgcg gccatagtcg tggacgccga
     1321 cggctttata gccgacgcga acaggacggc ccgcgggctc ttccctcggc tcgacgagga
     1381 cgggtcgtgc cttccactat cggaactcgg gagcgcgtac ccggagctgg cccggctacc
     1441 cgctccggga accttggccg acaccgcctc attacgcctt cgggtcggtg aagaagagcg
     1501 ggccttcgac gtgcaggcgt cgaacatcag cggccggtcg ggacggaacc gcggctcggt
     1561 catcctactg tacgacgtga ccgagcgcga acggctccag gcttgcctga tcgagcaggc
     1621 ctcgacggac gacctgacga gggtggcgaa ccggcggcgc ttctacgagg tcgccgaggc
     1681 cgagctcgag cgggccgggc gccacggccg ccccatcggc ttcgccatcc tggacatgaa
     1741 cggcttcaag gacatcaacg acggctacgg ccaccaggcc ggcgacgacg ccctgcgtct
     1801 cgtggccagg ctatgcgtcg gcgagctgcg gtcctgcgac ctggtcggcc gcgtgggcgg
     1861 cgacgaattc gcgttcgtgc taccggagag cgacgaggag ggagcggccg ccgccgccgg
     1921 caagctccgg agactcgtcg gcgcggcgac cctcaccgtc gggacggcga ccgtgcggct
     1981 gtccgcggcg gtcggctcgg taggctgctc cggcccggtc tatcccgacc tggccgagct
     2041 ccttacgata gccgaccgac gcatgtacgc cgacaagccg cgcttcgccg ccgtgaaggc
     2101 taacaaggcg gccaagacgg ccgccccccg ctagcctttc ttgcccagat ccgccagtcc
     2161 ggcgcccggc ctggtcatgc cctcgagggc ggccgcgact atatcgttgc gggcggcggc
     2221 cacagcggtc gggtggatgg ccgagatcgg ctcccggtcg ccacccgacc cggtcaccac
     2281 cgagacgtga tcggtcgggt agagcgccag ggtcttggcg aacggctcga acagcttctc
     2341 gaagttgtcg aggacccagg cccgcctcgc ctcgccgccg gcggccttca tcgtctcgag
     2401 cgtaaccgcc agttgctcca gctcggcctc ggctacctcg cgccagccgg agacctcagc
     2461 cctggcctcg aggatggact tctccttctc cgcgagcgcc ggggtgacga tctcggccct
     2521 gtagcggttc ttgagcatct cgatgttctg cctctcggcc tcgagctcgg ccttatagcc
     2581 ggagactctg gcccgcgcgc cggcggactc ctgctcgacg cctaccttct ggcgctgagc
     2641 ctcctcggcg agcttgaccc tcacctcggc cttgagcctc gtcagctcgt tgcccaggtt
     2701 cttgacctcg gtctccgcgc gcctggcctc cgcctgctcg gcgctcgccg cgcgcgcctc
     2761 ggcctgagcc tggcgggcct tggtctcggc gttggccgac tggatgcgtt tgaggagggc
     2821 gatgtacaga tccggctcct cgacgcccgg tagccgatga tcgtcgacgt cggcgatatt
     2881 catcgtcgtg atctcgaggc caatgttctc taggtcgttc ttgcagacgt tgatcatctt
     2941 ggcgacgagt atgtccttat ccttcatcac ctgctcgggc gtcatcgagg ctatggagtc
     3001 gcgcaggtgg ccctcgatga tgtcgccggc tatcttggtc aggttctcgc ggttcgaggc
     3061 tatgtccagg atgcgcgtca ccgcgtgcga gcggcccgtg tcgttcccgg caatggcgaa
     3121 cgagacggta gccttgacgt tgagcggcac gatgccggcc gctatcgccg agtcgacgac
     3181 cacctctatc gtgatgggcg tcaggtcgaa cttactgaag cgctgcagga gcgggatgat
     3241 gaaagcccgc ccgccggaca gggtcctgaa gcccttgtcg ccgcccgagc cggtcacgat
     3301 gccgagttcg ttgccgccga cgatcttcat gttggtcagg agctggatca ggatagtgac
     3361 gccgaccagc ccgccgagcg cgattacgaa cgctatcatt tcgacacccc cccggaggcg
     3421 gccggcatgg ccagcgagcg cacgtccacg ccgaacgcgt cggcgaaggt gcgcaggaag
     3481 tcggcgaagg cccgcgggcc gcgattgacc gcgcccgaga agccttcctc gtcgttcatg
     3541 acgacgaggt tgtcgacggc cccggatccg gtggccgcca tataggcctc gtagagctcg
     3601 ggcagcttct gctgcaggaa catgacgctc gaggcatcgt cgccgtaggc cgccagcaac
     3661 ccggccttgg ccttgagtac ctcgttatgg gccgtctgcc gtatcgatac agccgagcgc
     3721 tcgccc
//
LOCUS       DAOPHV010000227         3716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_34005_length_3716_cov_4.747610, whole genome shotgun sequence.
ACCESSION   DAOPHV010000227 DAOPHV010000000
VERSION     DAOPHV010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34005_length_3716_cov_4.747610"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PLQ29_07000"
     CDS             complement(<1..627)
                     /locus_tag="PLQ29_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86432.1"
                     /translation="MATSIHARFLQDARTLLEQASGLPELASELKEYGYTPGKFQEGF
                     KLLEEAEVLSRRKAVEYGEKYESSAKLVKAWKGANVLYQKALKVARVALRDAPKSTEA
                     LGLGGPRNLSVSGWYEQAGDFYANLLANDGYVRALGAFGYSRERLVEERRVIEAVMDT
                     RDETAIEAAQAKAVTAARDRKLKELDDWVSDLRAICEVAFYEKRDELPK"
     gene            complement(1162..1899)
                     /locus_tag="PLQ29_07005"
     CDS             complement(1162..1899)
                     /locus_tag="PLQ29_07005"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HPG86433.1"
                     /translation="MIETRRFDDEAAWIGAVLADAAGAVQEAEAEGRRAVLCLAGGRT
                     PEPAYRAVAAWLAARARGPGRPARPVLLVPGDERLEPRGPSDLNDTMLRRAFAPALDL
                     GAAELAAWNVSAGERAAVAAMAALVASIASGLPSAAPLFDRCYLGLGADGHVAGVFPG
                     SPAARRAGSRSAPIAVAGTAPEEPRARVSLSLPTLASTRGTRFIVRAAGKEDALRRLA
                     SGDPACPAVAAAAEGALAFVLARQARP"
     gene            2020..3312
                     /locus_tag="PLQ29_07010"
     CDS             2020..3312
                     /locus_tag="PLQ29_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HPG86434.1"
                     /translation="MRIDECVYARGGATDSIGDEELAAALIEALAAAGPRHRVLAVPP
                     DGTRSHARAGILLKAAYDYYGDALAAVMPALGTHRPMTDAAVEAVFPGVPPELFRPHD
                     WRRDVVELGRVPAAFVREASEGLCGFDWPAQVNRLVADGGFDLVLSLGQVVPHEVVGM
                     ANYSKNLFVGLGGKDGIDRSHWLGAAYGIERTLGRVDTPVRAVLDYAQERFLGPIPAL
                     YAMTVVGPDGRGGEATRGLFVGGGREAFSRAAALSAEVNIEYLERPADKVVVYLDPGS
                     FKSTWLGNKAVYRTRLAIADSGELLVIAPGVDSFGEDKSIDETIRDYGYRGSEAVRAL
                     VESGELSGDLAGAAHLAHGSPDGRFAVTYAAGGLSAADLAGVGYAWADWREALRRYDP
                     ASLADGWNEVGGEEIFFVRNPALGLWATAERYASLGPA"
BASE COUNT          584 a         1183 c         1339 g          610 t
ORIGIN      
        1 cttcggtagc tcgtcgcgct tctcgtagaa ggccacctcg cagatcgcgc ggaggtcgga
       61 gacccagtcg tccagttcct tgagcttccg gtcgcgggcg gcggtaaccg ccttggcttg
      121 ggcggcctcg atagccgtct cgtccctcgt atccatgacg gcctcgataa cccgccgttc
      181 ctcgacgagg cgctcgcggg aatagccgaa ggcgcccagg gctcgtacgt agccgtcgtt
      241 ggccaacagg ttcgcgtaga agtcgccggc ctgctcgtac cacccggaga cgctcaggtt
      301 gcgcgggccg cctagaccga gagcctcggt gctcttgggg gcgtcgcgca gggccacgcg
      361 ggccaccttc agggccttct ggtacaggac gttggcgccc ttccaggcct tgacgagctt
      421 ggcgctgctc tcgtacttct cgccgtactc gaccgccttg cgcctggaca gaacctcggc
      481 ttcttcgagc agcttgaacc cttcctggaa cttgcccggc gtgtagccgt actccttcag
      541 ctccgaggcc agctccggta gccccgatgc ctgttccagg agagtcctgg cgtcctgaag
      601 gaacctggca tgaatcgatg tggccatcgc ccacctccaa acgtgtattg gtgccgccaa
      661 tgacggcttt ttgtactcgg gagccgtgta gccggacatg gaagcggcct tgcctggccg
      721 gggagcgata ttcggcgatc gatgagcgat attcggcgat caatgggcga tcttccgaga
      781 tcgatgggcg atcttccggg atcgatgagc catgttcagg gatcgatgag cgatattcgg
      841 cgatcgatga gcgatattcg gcgatcgaag agtgatattc ggcgatcgat gggcgatgtt
      901 ccgggatcga tgggcgatgt tccgggatcg atgggcgatg ttccatgatc gatccgtcat
      961 ggtcggatgg aatcaggggg caggcgtcgc ttgctatgat taggagcctc cggtcagcgc
     1021 gggttcgaca cggcgaattg ctgcaggctc ccgatgtgga tgcttccgtt cgcccgtggc
     1081 ggctggaagc catgggctca gtgtaggccg gatcagggat tcgtcaaggg aaagttctga
     1141 tagggagtgg cgtgaagccg attatggccg cgcctgtcgg gcgagcacga acgccagagc
     1201 gccttcggcc gcggcggcga ccgccgggca ggccgggtcg ccggaggcca ggcggcggag
     1261 cgcgtcctcc ttgccggcgg cgcggacgat gaagcgcgtg cctcgggtcg aggcgagggt
     1321 cgggagggac aggctgacgc gggcgcgcgg ctcctcgggc gccgtaccgg cgacggctat
     1381 gggcgcggaa cgggaaccgg cccgacgggc ggccgggctg ccggggaaga cgccggcgac
     1441 gtggccgtcg gcgccgaggc ccaggtagca gcggtcgaag agcggcgcgg ccgacggcag
     1501 gccggaggcg atggacgcga cgagggcggc catcgccgcg acggcggcgc gctccccggc
     1561 ggagacgttc caggcggcca gctcggccgc gcctaagtcc agggccgggg cgaaggcgcg
     1621 cctgagcatc gtgtcgttca ggtcggaggg cccgcgtggc tcgagccgct cgtctccggg
     1681 cacgagcagg acgggccgcg cgggacggcc ggggccgcgc gccctggcgg ccagccacgc
     1741 ggcgacggcg cgataggccg gctccggcgt ccggccgccg gccaggcata ggacggcccg
     1801 ccgcccctcg gcctcggcct cctgtacggc cccggccgcg tcggccagga cggcgcctat
     1861 ccaggccgcc tcgtcgtcga agcgtctcgt ctcgatcatg gccagcagta tgcggccagc
     1921 cggcccgtag cgcaagcggg cttaaaaccg cgggcgcttc cgtggtatca tacgcccgcc
     1981 ctatcgtcgc ctcggggata gcggcttaaa ggaatgacga tgcgaatcga cgaatgcgta
     2041 tacgcgcgcg gaggggcgac cgactccata ggcgacgagg agctggccgc tgccctgatc
     2101 gaggcgctcg ccgccgcggg gccgcgccac cgggtcctcg cggtgccgcc cgacggtacg
     2161 cggagccacg cccgggccgg gatcctgctc aaggccgcct acgactacta cggcgacgcg
     2221 ctcgcggcgg tgatgccggc cctcggcacg catcgcccga tgaccgacgc ggccgtcgag
     2281 gcggtgttcc ccggcgtgcc tcccgagctg ttcaggccgc acgactggcg ccgagacgtg
     2341 gtcgagctgg ggcgcgtccc ggcggccttc gtccgcgagg cttccgaggg cctgtgcggc
     2401 ttcgactggc cggcccaggt caaccgcctc gtcgccgacg gcggcttcga cctggtgctg
     2461 tcgctcggcc aggtggtgcc gcacgaggtc gtcggcatgg ccaactactc gaagaacctg
     2521 ttcgtcggcc tgggaggcaa ggacggcatc gaccgctcgc actggctcgg cgccgcctac
     2581 ggcatcgagc ggaccctcgg ccgggtcgat acgccggtcc gggcggtgct cgactacgcc
     2641 caggagcgct tcctgggccc catcccggcg ctctacgcga tgacggtcgt cgggccggac
     2701 gggcgcggcg gcgaggcgac gaggggcctg ttcgtaggcg gcggccggga ggccttctcg
     2761 agggcggcgg ccctgtcagc cgaggtcaat atcgagtatc tggagcggcc ggctgacaag
     2821 gtcgtcgtct acctggaccc cggctcgttc aagtccacct ggctcggcaa caaggccgtc
     2881 tatcgtacca ggctggcgat tgccgactcc ggcgagctcc tggtgatagc ccccggcgtc
     2941 gatagcttcg gcgaggacaa gagcatcgac gagaccatcc gcgactacgg ctaccgcggt
     3001 tccgaggccg tgcgcgccct ggtcgagtca ggcgagctgt ccggcgacct ggccggcgcc
     3061 gcccacctgg cccatggctc gcccgatggg cgcttcgcgg tgacctacgc ggccggcggc
     3121 ctgtccgcgg ccgacctggc cggcgtcggc tacgcctggg ccgattggcg cgaggcgttg
     3181 cgccgctacg acccggcgtc gctggccgac ggctggaacg aggtgggcgg cgaggagatt
     3241 ttcttcgtgc gcaatccggc gctcgggctg tgggccaccg cggagcgcta cgcctccctc
     3301 gggcccgcct gagccgcggt tcggaagaag cggtatccgc gaaaacgaga acaggatgac
     3361 cggatgcaag aaggatggcc cgatgagcgg gaatggatag ggagtttcgc gccaaaccac
     3421 gaaggcgcta tggcgcggag gggatagaaa taaagcggaa caagatgatc aagatttgga
     3481 caggatggac aagataataa aagctatagc ttaggtcttc atcttgtcca tcttgccttc
     3541 atcctgatca tcctgttctg ttttttacta ttccttcgtg ccttggtgtc ttggtgtgag
     3601 gccttgccta agaagatctc acaccaagcc acgaaggcgc tatggcgcgg aggggatagg
     3661 gataaagcgg aacaagatga tcaagatttg gacaggatgg acaagataat aaaagc
//
LOCUS       DAOPHV010000228         3704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_34153_length_3704_cov_3.039189, whole genome shotgun sequence.
ACCESSION   DAOPHV010000228 DAOPHV010000000
VERSION     DAOPHV010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34153_length_3704_cov_3.039189"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..448)
                     /gene="rpsO"
                     /locus_tag="PLQ29_07015"
     CDS             complement(179..448)
                     /gene="rpsO"
                     /locus_tag="PLQ29_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPG86435.1"
                     /translation="MAVTKEDKARLVKEYGTSEKDTGATKVQIALLTERISGLTEHFK
                     VNKKDTNSKRGLMILIGQRRRLLKYLQRKDLNTYRDICDKLGIRK"
     gene            complement(474..1274)
                     /locus_tag="PLQ29_07020"
     CDS             complement(474..1274)
                     /locus_tag="PLQ29_07020"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HPG86436.1"
                     /translation="MRVVDWAEAVSGRAPMPEHSAATIGVFDGLHLGHRALIERVLAK
                     GPALAPVIVTFRDNPKRITRPHRFSGSLFTLEQKMAALEEAGAEVCVLIDFSGNFSKL
                     AGSEFVSALGSIGVRSFVIGSDFKCGHRLSTDAEGLKAVAASFGADTEIVGPVTLGGE
                     PVSSSRIRAAIANGRTDLALAMLGRPYTLDLRRLEIGYERGSAAFSFKGSGLVEPAPG
                     RYEATEICVAGSGAVEATLRRDGTVHWARTALCADHEPAFLAFGSRIA"
     gene            complement(1271..2170)
                     /gene="truB"
                     /locus_tag="PLQ29_07025"
     CDS             complement(1271..2170)
                     /gene="truB"
                     /locus_tag="PLQ29_07025"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPG86437.1"
                     /translation="MTDEPWSPGLGFAVVDKPEGVTSYKLIASLGRRLGDRVKCGHAG
                     TLDSFASGVLVCLFGRYTRLSDYFMGAGKGYEASVRFGEETDTLDPEGSVVATAPVPS
                     LEALEAALPAFRGDILQAPPAYSAVHVDGERAYERALRGEDVKPALRPVTIRSLELLS
                     YEGGVARLRVSCTKGTYIRSLARDLALACGSRGRLSALRRTFSGPFTAEAAVRADAFG
                     PDSLRFLSEPDARGLNLGVVRLAGEEAHAFSNGLPLFRLSTFAGLAGQGPVVAFDEAG
                     RLLGVVSRASGAWRYSFVLGGEV"
     gene            complement(2167..2514)
                     /gene="rbfA"
                     /locus_tag="PLQ29_07030"
     CDS             complement(2167..2514)
                     /gene="rbfA"
                     /locus_tag="PLQ29_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPG86438.1"
                     /translation="MDEIRRKRVEETLRTEVSALLMRGEIKDPRVDTFLSITRVEVAR
                     DGSHAKLYVSTFRESGALDEGVAGLNNAAGFIQAGISKRLRIRLTPRLVFIPDTGIKE
                     GFELGEKIKGLFK"
     gene            complement(2507..>3704)
                     /gene="infB"
                     /locus_tag="PLQ29_07035"
     CDS             complement(2507..>3704)
                     /gene="infB"
                     /locus_tag="PLQ29_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPG86439.1"
                     /translation="NKIARPVAIPDRVKTQLSELQLVPEDWGGQTMFCEISALQKKGI
                     DELLDGILLTAEVLELNANYDCRAEGKVIESRIDHGRGIVSTIIIERGTLRVGDSFVA
                     GVFPGKVRAMFDDKGRKIDEATPSTPVEVIGFEGVPSAGDPFEAVADEKIARSFSDKR
                     QELKRFEEAKTIKKVTLDNLYDTINEGAVQELKVLIKGDVHGSVEALKSSLEKLSTNE
                     VRLAAIRAAAGAINESDVDLASASSAIIIGFNVRPTPKAKALADQEKVEIRKYNVIYK
                     AVEEIQQAMEGMLAPEIKESDIAEAEVREVFKVPKIGAVAGCFVLSGTVKRNASVRVI
                     RDGVELHACKISTLKRFKDDAKEVAAGYECGIGLTDWTDIKVGDVLEVFELIKISRKL
                     GDTVHG"
BASE COUNT          647 a         1219 c         1137 g          701 t
ORIGIN      
        1 caaagcgacc aaagactgat ccgttggcct gtttggccat gcgcccggtc tcgaacacga
       61 ggtcttcgcc gttaattcta tacgtgctgg tatgtgccat atgcgatggg tctccatggg
      121 ccgcgttcgg cctaatgacg aaagagcccg aaccgtcgtt cgggctccga taagacgatt
      181 atttacggat gcctagcttg tcgcagatgt cgcgatacgt gttcaggtcc ttgcgctgca
      241 ggtacttgag caagcgcctc cgctggccga tgaggatcat cagcccgcgc ttggagttgg
      301 tgtccttctt gttaaccttg aagtgctcgg tgagaccgct gatgcgctcg gtgagcaacg
      361 cgatctggac cttggtcgcg ccggtgtcct tctcgctggt accatactcc ttgacgagcc
      421 tggccttatc ctctttcgtc actgccatgt ctataaactc cttattgccg ttatcatgcg
      481 atcctcgagc cgaacgcgag gaaggcgggc tcgtgatcgg cgcataacgc cgtcctcgcc
      541 cagtgtaccg tgccgtcccg ccgcagagtc gcctccacgg cgccgctacc ggcgacgcat
      601 atctccgtcg cctcgtacct gccgggcgcg ggctccacca aaccgctacc cttgaacgag
      661 aacgccgcgg agcctcgttc atatccgatt tcgagccgcc ttagatccaa ggtgtagggg
      721 cgaccgagca tggcgagtgc caggtccgtc cttccgttcg cgatagccgc gcgtatccgg
      781 ctcgagctga ctggctcccc gccaagcgtg acagggccga cgatctccgt atccgcgccg
      841 aaggaggccg ctacggcctt gagaccctcg gcgtcggtgg atagtcggtg cccgcacttg
      901 aagtcgctac cgatgacgaa cgaacgaacg ccgatggacc ctagggccga aacgaattcg
      961 ctcccggcca gtttactgaa attcccggaa aagtcaatca ggacgcagac ctccgcaccc
     1021 gcttcctcga gggcggccat cttctgctcg agggtaaaga ggcttccaga gaagcgatgc
     1081 ggcctcgtta tccgtttcgg gttgtccctg aaggtgacta tgaccggcgc cagggcgggc
     1141 cccttggcaa ggacacgctc gatgagcgcc cgatgcccga ggtgcaagcc atcgaagacg
     1201 cctatcgtgg cggccgagtg ctcgggcatg ggcgcacggc ccgagaccgc ttcggcccaa
     1261 tctacgaccc tcacacctcg cccccgagca cgaaggagta gcgccatgcg ccgctcgcgc
     1321 gagagacgac gccaaggagc cggccagcct cgtcgaacgc gacgaccggt ccctgccctg
     1381 ccaggcccgc gaaggtagac agcctgaaca agggcagtcc gttcgagaac gcgtgggcct
     1441 cttcgcccgc caggcggacg acgccgaggt taaggcctcg agcgtcaggt tcggacagga
     1501 agcgcagact atcgggcccg aaggcatcgg cgcgtacggc cgcctccgcg gtgaacggcc
     1561 ccgaaaaggt gcgccggagc gccgaaagcc taccacgcga gccgcaagcc agcgccaggt
     1621 ccctggctaa cgagcgaata taggtaccct tggtgcacga gacgcgcagc ctggccacgc
     1681 cgccttcgta ggataacagc tcgagggagc gtatcgtcac gggtcgcagc gccggcttca
     1741 cgtcctcgcc ccgcagggcg cgctcatagg cgcgctctcc gtcgacgtgg acggccgagt
     1801 aagccggagg agcctgtagt atgtctcccc tgaaggccgg cagggccgcc tcgagggctt
     1861 cgaggctcgg taccggtgcc gtggcgacga cggagccctc cggatcgagg gtgtcggtct
     1921 cctcgccgaa gcgcacgctc gcttcgtagc ccttgccggc gcccatgaag taatccgata
     1981 gcctggtgta acggccgaac aggcagacga gcacgcccga ggcgaacgag tcgagcgttc
     2041 cggcatggcc gcacttgacc cggtcgccga gccggcggcc cagggaggct atgagcttgt
     2101 acgaggtaac gccctcgggc ttatcgacaa cggcgaagcc caagcccggc gaccagggct
     2161 cgtctgtcac ttgaacaagc ccttgatctt ctcgcccagc tcgaagcctt ctttaatacc
     2221 cgtatcgggg atgaatacca gcctcggcgt cagacgtatc ctgagccgct tcgatatgcc
     2281 ggcctggatg aagcccgccg cgttattgag cccggcgacg ccttcgtcga gggcgcccga
     2341 ctccctgaag gtcgacacgt acaacttggc gtgcgagccg tcccgcgcga cctcgaccct
     2401 ggtgatggag aggaaggtat cgaccctggg atccttgatc tcgcctcgca tcagcaaggc
     2461 cgagacctcg gtcctcaggg tctcctcgac gcgcttacgc cttatctcat ccatgtaccg
     2521 tatcgcccag cttcctggag atcttgatca gctcgaagac ctcaagcacg tcgccgacct
     2581 tgatgtcggt ccaatcggtc aggccgatgc cgcactcgta gccggcggcc acttccttgg
     2641 cgtcgtcctt gaagcgcttg agggtagaga tcttgcacgc gtgcagctcg acgccatcgc
     2701 gtatgaccct gacgctggcg ttccgcttga cggtgcccga gagcacgaaa cagcctgcga
     2761 cggcgccgat cttcggtacc ttgaagacct cgcggacctc cgcctcggcg atgtcggact
     2821 ccttgatctc cggagccagc atgccctcca tggcctgctg tatttcctcg actgccttgt
     2881 agatgacgtt gtacttgcga atctcgacct tttcctggtc ggccagggcc ttggccttgg
     2941 gcgtcggcct gacgttgaag ccgatgatga tcgccgacga ggcgctagcc aggtcgacat
     3001 cgctctcgtt gatggcgccg gccgcggccc ggatggcggc gaggcgcacc tcgttggtcg
     3061 acagcttctc gagcgaagac ttgagcgcct cgacggagcc atggacgtcg cccttgataa
     3121 gtaccttgag ctcctgcacg gcgccctcgt tgatggtgtc gtacagattg tcgagcgtga
     3181 ccttcttgat agtcttggcc tcttcgaagc gtttcagttc ctgacgcttg tcggagaagg
     3241 aacgggcgat cttctcgtcg gccaccgcct cgaaggggtc gccggcgctc gggacgccct
     3301 cgaagccaat cacctctacc ggcgtcgacg gggtggcctc atcgatcttg cggcccttgt
     3361 cgtcgaacat ggctcggacc ttgcccggga agacgccggc cacgaagctg tcgccgacgc
     3421 gcaacgtgcc gcgctcgatg atgatcgtcg acacgatgcc gcggccatga tctatccgcg
     3481 actcgatgac cttgccctcg gcgcggcagt cgtagttggc gttgagctcg agcacctcgg
     3541 ccgtcaggag tataccgtcg agcaactcgt cgataccctt cttctgcagt gccgagatct
     3601 cacagaacat cgtctggccg ccccagtcct cgggaactag ctgcagctcc gacagctgcg
     3661 tcttgacgcg gtccgggatc gccacggggc gggcaatctt gttg
//
LOCUS       DAOPHV010000229         3703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_34161_length_3703_cov_3.161732, whole genome shotgun sequence.
ACCESSION   DAOPHV010000229 DAOPHV010000000
VERSION     DAOPHV010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34161_length_3703_cov_3.161732"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PLQ29_07040"
     CDS             complement(<1..291)
                     /locus_tag="PLQ29_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HPG86440.1"
                     /translation="MKIVLVGAGSAQFGCGTLGDIFTSKRLTGSEITLHDINAEALAA
                     TLESAEATVKAHALKYTVNATTDRRAAFKGADFIISSIEVGNRFTLWEEDWKV"
     gene            complement(303..1181)
                     /locus_tag="PLQ29_07045"
     CDS             complement(303..1181)
                     /locus_tag="PLQ29_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPG86441.1"
                     /translation="MTVVAAPGKQLKPARILLGALVALLFAFFMAPFAMVVLNSSKTS
                     REIIYNAIALPESWGQLLTNVRLIFTNPTVDYLGAFWDSTVITFLSLVVIVLFSSMCA
                     WVLVRSKSVWSAVLFMAFVAAMVIPFQVLMYPLVRWMRVIGDALNFRLLGTVRGIVFA
                     YLGFGCPLSIFIFHGFIKNIPYELEESATIDGCSQGRTFFLVVFPLLKPIIVTVLILN
                     GIWIWNDYLLPLLVLGSNGVVQTIPIAVTAFAGAYLKQWDLILTSTLIAMLPIIVLYL
                     FAQRYIIKGMVEGAIK"
     gene            complement(1178..2065)
                     /locus_tag="PLQ29_07050"
     CDS             complement(1178..2065)
                     /locus_tag="PLQ29_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPG86442.1"
                     /translation="MRRHRLVSILFVAPCVFAFAMNILIPFGFGLYYSMTDWNGVNPD
                     INFVGFDNFAGLFSSPDFIYSFLITVAYTAINIVLVNAVSFGLSLIVTGRIRGRNFLR
                     AGFFIPYLIGGIVLGYIWQFIFNNALVAVGKSLSIAALQTSFLSRPGSVIWIMAVVNT
                     WQYAGYIMLIYVAAIQGIPASLMEAAEVDGANYPTRIFKILMPMMANAFTISLFLTLT
                     TSFKQFDMNLTLTNGGPATRFMDTPIKASQLLAMNIFSTATANRMAEAQAKAVVLFVA
                     LVVVALLQVAANKKKEVEL"
     gene            complement(2148..3428)
                     /locus_tag="PLQ29_07055"
     CDS             complement(2148..3428)
                     /locus_tag="PLQ29_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPG86443.1"
                     /translation="MRRPFVRIVSIVAAIVCIVAVASCSKAEPKQAKGVEINMFQLKV
                     EIKDAIDGYAAAYSAAHPGVTVKVETLGGGGDYGGALKAKSQAGQMPDIFVIEGLGGY
                     NIWKDYIADLSGEPWVKDTDLAMKVDGKIVGFPVAIEGYGLAYNADILAKAGIDPAAL
                     TTRAAYEKALAKLDSMKKELGITAPVSMAASVAGGMWWVAAQHNLACYWGGGLAFDDT
                     SVIDKALKGELDEARFTQYAKYLQLLFKYADQNILLNGSYDDQVGAFAQGKTAFLHQG
                     NWVDPNLKQLGVTFTIGYAPHAFLDTEQKGLYLFAPSWYCVNSKSPNADAAKAFLTAM
                     ATTPEGHDYMVNKAGMIPAFKSVTLKPAGQLSQALMAANAKGGNYGVFFGMLPDGAGQ
                     NVFGPIFDLFAQNQKDLPRFISDMKKAVADLPKM"
     gene            complement(3523..>3703)
                     /locus_tag="PLQ29_07060"
     CDS             complement(3523..>3703)
                     /locus_tag="PLQ29_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86444.1"
                     /translation="ACVVACVVACVVACVVACVVACYAEGLARAGPPQPVGFVLIARY
                     SEADGSIRRAIDSVS"
BASE COUNT          707 a         1060 c         1240 g          696 t
ORIGIN      
        1 caccttccag tcctcctccc agagcgtgaa gcggttgcct acctctatcg acgagatgat
       61 aaaatccgcg cctttgaagg ccgcgcgcct atcggtcgtg gcgttcaccg tatacttgag
      121 cgcatgcgcc ttgaccgtcg cttccgccga ctcgagggtg gcggcgaggg cctcggcgtt
      181 gatgtcgtgt agcgttatct ccgagccagt cagtcgcttg ctcgtgaaaa tgtcgccgag
      241 cgtcccgcag ccgaactggg cgcttccggc gccgactaat acgatcttca tggttcctcc
      301 gtctatttta tggcgccttc caccataccc ttgatgatgt agcgctgagc gaacaggtac
      361 agcacgatga tcgggagcat cgcgatgagc gtcgaggtca ggatcaggtc ccattgcttg
      421 aggtaggctc cggcgaaggc cgtgaccgct atcggtatgg tctggacgac cccgttcgag
      481 ccgagcacga gcagcggcag gaggtagtcg ttccagatcc agatgccgtt caggatcagc
      541 accgtcacga tgatgggctt gaggagggga aagacgacca ggaagaaggt cctgccctgc
      601 gagcagccgt cgatggtggc cgactcctcg agctcgtacg ggatgttctt gatgaagccg
      661 tggaatatga agatcgacag cgggcagccg aagcccaggt acgcgaagac gatgccgcgc
      721 acggtgccga gtagcctgaa gttgagcgcg tcgcctatca cccgcatcca gcgcacgagc
      781 gggtacataa gcacctggaa cgggatgacc atcgcggcga cgaaggccat gaacagcacg
      841 gccgaccata cgctcttgct gcgcacgagt acccaggcgc acatcgacga gaacagcacg
      901 atcacgacga gcgagaggaa ggtgatcacg gtgctgtccc agaaggcgcc caggtagtcg
      961 acggtggggt ttgtgaagat gagcctgacg ttcgtcagga gctggcccca gctctcgggc
     1021 agggctatgg cgttgtagat gatctcgcgg gaggtcttgg acgagttgag cacgaccatc
     1081 gcgaagggcg ccatgaagaa tgcgaataag agggccacca aggcgccgag caggatccgc
     1141 gcgggcttga gctgtttccc cggggcggcc acgacggtca tagctccacc tccttcttct
     1201 tgttcgcggc gacctgcagg agggccacca cgacgagggc gacgaacagg acgacggcct
     1261 tggcctgggc ctccgccatc cggttggcgg tggccgtgct gaagatgttc atagccagga
     1321 gttggctcgc cttgatcggc gtatccatga agcgggtggc ggggccgccg ttcgtcagcg
     1381 tcaggttcat gtcgaactgc ttaaacgagg tggtgagcgt caggaagagc gatatggtga
     1441 aggcgttggc catcatgggc atgagtatct tgaagatgcg cgtcgggtaa ttggcgccgt
     1501 ctacctcggc ggcctccatc agcgaggcgg ggatgccctg gatggccgcg acgtagatca
     1561 acatgatgta gccggcgtac tgccaggtat tgaccaccgc catgatccag atcaccgaac
     1621 cgggcctgct caggaaggag gtctgtagcg ccgctatcga cagggacttt ccgacggcta
     1681 ccagtgcatt attgaagatg aactgccaga tgtagccgag cacgatcccg ccgatcaggt
     1741 aggggatgaa gaagccggcc ctaagaaaat tgcggccgcg tatccggccc gtcacgatga
     1801 gcgacagccc gaagctgacg gcgttgacga gcacgatgtt gatggcggta tacgccaccg
     1861 tgataaggaa cgagtagatg aaatccggcg acgagaaaag gccggcgaaa ttgtcgaacc
     1921 ctacgaagtt gatatcggga ttcacgccgt tccagtctgt catcgagtag tagaggccga
     1981 agccgaacgg gatgagtatg ttcatcgcga atgcgaatac gcacggggcg acgaatagta
     2041 tgctgacgag gcggtgtcgt ctcatggcta cctcccggaa cgagcggaat ggtagcccgg
     2101 tccggcgcct aggtggcgcg ccgggccggg ctcgtttaag gagttcttta catcttgggc
     2161 aggtcggcga ccgctttctt catgtcggaa atgaagcggg gcagatcctt ctggttctgg
     2221 gcgaacaggt cgaagatcgg accgaacacg ttctggccgg ccccgtccgg gagcatgccg
     2281 aagaacacgc cgtagttgcc gcccttggcg ttggcggcca ttagggcctg gctgagctgg
     2341 ccggccggct tgagcgtcac cgacttgaag gccgggatca tgcccgcctt gttgaccatg
     2401 tagtcgtggc cctcgggtgt ggtggccatg gcggtcagga aggccttggc ggcgtcggcg
     2461 ttcgggctct tggagttgac gcagtaccag ctgggcgcga agaggtacag gcccttctgc
     2521 tcagtgtcga ggaaggcgtg gggcgcgtag ccgatcgtga aggtcacgcc gagctgcttg
     2581 agattcgggt ctacccagtt gccctggtgc aggaaggccg tcttgccctg ggcgaaggcg
     2641 ccgacctggt cgtcgtagct gccattcaag aggatgttct ggtcggcgta cttgaacagg
     2701 agctgcaggt acttggcgta ctgggtgaag cgagcctcgt cgagctcgcc cttcagggcc
     2761 ttgtcgatga cgctggtgtc gtcgaaggcc aggccgccac cccagtagca ggcgaggttg
     2821 tgctgcgcgg ctacccacca catgccgccg gcgacggacg cggccatcga taccggcgcg
     2881 gtgatcccga gctccttctt catggagtcg agcttagcga gcgccttctc gtaggcggcg
     2941 cgcgtggtca gcgcggcggg gtcgatgccg gccttggcca ggatgtcggc gttataggcg
     3001 agtccgtaac cctcgatggc caccgggaag ccgacgatct tgccgtcgac cttcatcgcc
     3061 aggtcggtgt ccttgaccca gggctccccg ctcaggtcgg ctatgtagtc cttccagatg
     3121 ttgtagccgc cgaggccctc gatcacgaag atgtcgggca tctggccggc ctgggacttg
     3181 gccttcaggg ctccgccgta gtctccgccg ccgcctaggg tctccacctt gacggtgacg
     3241 cccgggtggg cagcggaata ggccgcagcg tagccgtcga tggcgtcctt gatctccacc
     3301 ttcagctgga acatgttgat ctcgacgccc ttggcctgct taggctcggc cttcgagcag
     3361 gatgccacgg cgacgatgca tacgatcgcc gcgacgatcg agacgatgcg aacgaagggt
     3421 ctcctcatct ttccacctcc aacggttttt agcgcgtgct gttcagcgcc tgtaggtgta
     3481 acgtaacacc tacggctgag tatacgtgcg tttgaggatt cgtcaagata cagaatcgat
     3541 ggcgcggcgt atcgatccat cggcctctga gtacctcgcg atgagaacga aacctaccgg
     3601 ctggggaggg ccggcgcgcg cgaggccttc agcatagcag gcgacgacgc aggcgacgac
     3661 gcaggcgacg acgcaggcga cgacgcaggc gacgacgcag gcg
//
LOCUS       DAOPHV010000230         3698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_34210_length_3698_cov_4.580565, whole genome shotgun sequence.
ACCESSION   DAOPHV010000230 DAOPHV010000000
VERSION     DAOPHV010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34210_length_3698_cov_4.580565"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..801
                     /locus_tag="PLQ29_07065"
     CDS             142..801
                     /locus_tag="PLQ29_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86445.1"
                     /translation="MTPLMLALAAQAVVSVFLIVWTRSKVRRFLGADEELERIRREIG
                     SLIVELDATADRNVTVLEDRLRALKDSLAEADKRIALFGQERSRRQAEAVVYDRLGRA
                     GEPRRPVQAPQAASESAQPRQVLDADEPQVLRSSEKETGPLSVGQAAPSPISGAPSVP
                     FIRFSDKPLATDEPFADKVVSLARRGFSSDIIAARLGATIAEVDLALAIEREREARTK
                     E"
     gene            806..2521
                     /locus_tag="PLQ29_07070"
     CDS             806..2521
                     /locus_tag="PLQ29_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPG86446.1"
                     /translation="MRGVIGLDFGTTNTLCAWMDGDTPIIVPNDRGERATPSVVAVSG
                     SGEALVGSSARNQALADPASALFGVKRLLGKSQALVFGGRERAPEEAAAAIIGKVKRD
                     AESYLGFEADRAVITVPARYGDRQRRAVRDAAAMAGLKAARVMNEPSAAALAKAWLSA
                     RDESERLVLVYDFGGGTFDATALRARGGSCSVLSSEGDDALGGMDIDAAIYETVAERF
                     RLEFGIDPSADPYLARTLVDLCERAKIELSSRDEATIAVPFLRGAGGVVHPSVRLERA
                     AFEAIASPFIDRSLALVERVLRTAKAGVRDVDALVLSGGSSRIPLAKRRLAELLGRDA
                     DPRINPEEIVALGAAVEAARIEGRLKGLSFTDVCSRTFGLEIDGGSFVPLIVKDEPLP
                     AVGRRVFTTVEDYQRSVELHVLQGESERASDNASVGRFLLPGIRKATRGEPRIAVEFA
                     IDEGDMMIVRARDLDTGAEQSIAVFDGERDSRSPRDRVLALAQIVRRESAEAIVDAAL
                     GAEIGDVLAMAESCGDDDEKAAMVATLLEGLLSELSARTGRSEADARRPSELPFRGRA
                     AAGGA"
     gene            2518..3153
                     /locus_tag="PLQ29_07075"
     CDS             2518..3153
                     /locus_tag="PLQ29_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPG86447.1"
                     /translation="MSERAMYLALGLEPGAPSEEIKRAYRNLSLRLHPDASGDPSTAR
                     RFSMVAKAYQTLRVAERERPATLRPLEEPGRTDLFALGVELSTHPDPARRRAAARRLG
                     LTGKRSAWVFLRKGLYDRDEEVAAACVRAAAVLGLAQGSGEVADAYQRGGPGLRDAML
                     DAARATRDPLFSAAIEVARTDEDPRRRSIAYRLAMESSQSAVAPARGEEVS"
     gene            complement(3150..>3698)
                     /locus_tag="PLQ29_07080"
     CDS             complement(3150..>3698)
                     /locus_tag="PLQ29_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPG86448.1"
                     /translation="EETIETDVRVVAATNRDLKDEIAAGKFREDLYYRLNVVNIHVPP
                     LRERKDDIPLLAGAFLREFAEDNGKGVEGFDPKARAALYAYAWPGNVRELRNCIESAV
                     VMASGGLVTLDDLPPGVSTSASDSDIRVPPGSSLADAERILIRETLAAQGGNKSRTAE
                     ILGIGRKTLYQKIQDYGLETSA"
BASE COUNT          554 a         1266 c         1311 g          567 t
ORIGIN      
        1 tcggtcaacg gcgacggagg ctcgggagcc ggctcccagg gcggcgagcg gcgccgcgag
       61 gagaagcctg ccgaggacga cgaggccgag acgatcagcg atccgtcgct cggcggccac
      121 gtcgatatta cgggttaatc gatgacgccc ctcatgcttg ccttggccgc ccaggccgtc
      181 gtctccgtct tcctcatcgt atggaccagg tcgaaggtcc gtcgcttcct gggcgccgac
      241 gaggagctgg agcgcatacg gcgcgagatc ggttccctga tcgtcgagct tgacgcgacc
      301 gccgaccgga acgtcacggt gctcgaggac cggctccggg ccctcaagga ttcgctcgcc
      361 gaggccgaca aacgtatcgc cctcttcggc caggaacggt ccaggcggca ggccgaggcg
      421 gtcgtctacg ataggctcgg tcgtgccgga gagccgcgtc ggccggttca ggcgccgcag
      481 gccgcgtctg agtcggcgca gccccgtcag gtcctcgacg ccgatgagcc acaggtcctt
      541 cgatcgtccg agaaggagac agggccgctg tcggtcgggc aggcggctcc gtcgccgata
      601 tcgggcgccc cctcggttcc cttcatccgg ttctccgata agcccctcgc gaccgacgag
      661 ccgttcgccg ataaggtagt atcgctcgcc cggaggggct tctcgagcga tatcatcgcg
      721 gccaggctcg gcgcgaccat cgccgaggtg gacctggcgc tcgcgatcga acgggagcga
      781 gaggctcgaa cgaaggaata gcggcgtgcg cggcgtgata ggcctggatt tcggaaccac
      841 caataccctc tgcgcctgga tggacggcga tacgcccatc atcgtaccga acgaccgggg
      901 agaacgggcg acgccgtccg tcgtcgccgt gtcgggatcc ggagaagcgc tcgtcggctc
      961 gtccgcccgt aaccaggccc tggccgatcc cgcctcggcc ctgttcggcg tcaagcgtct
     1021 gctcggaaag tcccaggcgc tcgtgttcgg cgggagggag cgtgcgccgg aggaagccgc
     1081 agcggccatc atcggtaagg tcaagcgcga cgccgagtcg tatctaggct tcgaggcgga
     1141 ccgagccgtg ataacggtgc cggccaggta cggcgaccgc cagcgcaggg ccgtccgcga
     1201 cgccgccgcg atggccggcc tcaaggccgc ccgcgtgatg aacgagccga gcgccgccgc
     1261 cctggccaag gcctggctgt cggcccgcga cgaatcggag cggctcgtcc tggtgtacga
     1321 cttcggcggc ggcaccttcg acgccaccgc gctgcgggcc aggggcggct cgtgctcggt
     1381 cctgtcgagc gagggcgacg acgcgctcgg cggcatggat atcgacgcgg cgatctacga
     1441 gacggtggcc gagcgcttca ggctcgagtt cggcatcgat ccgtcggccg atccctacct
     1501 ggccaggacc ctcgtcgacc tgtgcgaacg ggccaagatc gagctgtcga gccgcgacga
     1561 ggcgacgata gccgtgccct tcctgcgcgg cgccggcggc gtcgtccacc cgagcgtccg
     1621 actggagcga gccgccttcg aggcgatagc ctcgccgttc atagatcgta gtctggccct
     1681 cgtcgagcgg gtgctccgca ccgccaaggc gggagtccgc gacgtcgacg cgctcgtact
     1741 atccggcggc tcgagccgca tccccctggc caagcgccgt ctcgccgagc tcctggggcg
     1801 cgacgccgac cccaggatca accccgagga gatagtcgcg ctcggggcgg ccgtcgaggc
     1861 cgctaggata gagggccggc tcaagggact gtccttcacc gacgtctgct cgcgcacctt
     1921 cggcctcgag atagacggcg gatccttcgt cccgctgatc gtgaaggacg agccgctgcc
     1981 cgccgtcggc cgccgcgtct ttaccacggt cgaggactac cagcgctcgg tcgagctgca
     2041 cgtgctccag ggcgagtcgg aacgggcctc ggataacgcc tcggtagggc gtttcctctt
     2101 gccgggcatc cgcaaggcga ccaggggcga gccccgcatc gccgtcgaat tcgccataga
     2161 cgagggcgac atgatgatag tcagggcccg cgacctggat acgggcgcgg aacagtccat
     2221 cgcggtgttc gacggggagc gtgactcgcg ctcgccgcgc gacagggtgc tcgccctggc
     2281 gcagatagtc aggcgggaat cggcggaggc catcgtcgac gcggccctgg gcgccgagat
     2341 cggggacgtg ctggcgatgg ccgagtcatg cggcgatgac gatgagaagg ccgccatggt
     2401 cgcgaccctg ctcgaggggc tcctatccga gctgtccgct aggacggggc gttctgaagc
     2461 cgacgctcgg cgaccgtcgg agctaccgtt ccggggccgc gcggccgcgg gtggcgcatg
     2521 agcgagcgcg cgatgtacct ggcgctcggc ctggagcctg gggcgccgtc ggaggagatc
     2581 aagcgggcgt atcgcaacct gagcctgcgg ctgcacccgg acgcgtccgg cgatccgtcg
     2641 acggcccgac gcttctcgat ggtcgccaag gcctaccaga cgctcagggt cgcggagcgc
     2701 gagcgtccgg cgacgttgcg gccactcgag gagcccggac gaaccgacct gttcgccctc
     2761 ggcgtcgagc tgtccacgca tcccgacccc gcgcgacgtc gggccgcggc caggaggctc
     2821 gggctgaccg gaaagcgcag cgcctgggtt ttcctgcgca aggggctata cgaccgcgac
     2881 gaagaggtgg cggccgcctg cgtcagggcc gccgccgtgc ttgggctggc ccagggctca
     2941 ggcgaggtag ccgacgccta ccagcgcggg gggccgggct tacgcgacgc catgctcgac
     3001 gcggcgaggg cgacgcgcga cccgctcttc tctgccgcca tcgaggtcgc caggaccgac
     3061 gaggatcccc gccgacgctc gatcgcctac aggctcgcca tggagtccag ccagtcggcc
     3121 gtcgcgccgg cccggggcga ggaagtctcc taggcgctcg tctcgaggcc gtagtcctgg
     3181 atcttctggt acagggtctt cctgcctatc ccgaggatct cggccgtgcg gctcttattc
     3241 ccgccctggg cggcgagcgt ctcccgtatc aggatgcgct ccgcgtcggc caggctcgag
     3301 ccgggcggca cgcggatgtc gctatcggag gcgctggtac tcaccccggg cggcagatcg
     3361 tcgagcgtga cgagtccgcc cgaggccatc acgaccgcgc tctcgatgca gttgcgcagc
     3421 tcgcgaacgt tgcccggcca ggcataggcg tagagggcgg cccgcgcctt ggggtcgaaa
     3481 ccctcgactc ccttgccgtt gtcctcggcg aattccctga ggaaggcacc agccaggagg
     3541 ggaatgtcgt ccttgcgctc cctgagcggc ggtacgtgga tattgacgac gttgagccta
     3601 tagtatagat cttcacggaa cttcccggcc gcgatctcgt ccttcaggtc gcggttcgtc
     3661 gcggcgacga cccttacgtc ggtctcgatc gtctcctc
//
LOCUS       DAOPHV010000231         3694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_34281_length_3694_cov_4.000550, whole genome shotgun sequence.
ACCESSION   DAOPHV010000231 DAOPHV010000000
VERSION     DAOPHV010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34281_length_3694_cov_4.000550"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PLQ29_07085"
     CDS             complement(<1..718)
                     /locus_tag="PLQ29_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPG86449.1"
                     /translation="MKYRTFFAVLLIAALAFTGCAKEEKAAPVAAKPVFKVGLVTDLG
                     GIDDKSFNQGTWEGVIRFGEENGLVKGTDYKYLQSGAEADYVPNLSTFADEKLDLIVA
                     PGFLFANAMGEVAEKYPQQKLMIIDDSSVQKPNVVCAVFAEHEGSYLVGVATGLKAKA
                     DGKKVVGFIGGMQFPLIEKFQAGFEQGVKAVYPEATILVDYAGDFGAPDKGQALAQKQ
                     YKAGAYIIFHAAGGTGNGMIK"
     gene            complement(832..1974)
                     /locus_tag="PLQ29_07090"
     CDS             complement(832..1974)
                     /locus_tag="PLQ29_07090"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPG86450.1"
                     /translation="MKHRRLVAIALALTLALSSCGGNDRAVATAKRAKPTIGMVFDIG
                     GRDDASFNRSAYDGLAMLARDSGGRIEGDPDADFGDALDIRVLESKHGGVDREQLLRS
                     LAEDGRDLVIAVGFLFADSIGSVASDYPETHFVIIDGTVPDLNASSSVTCVDFAEHEG
                     SFLVGAYAGLLAAAAGPDARVGFVGGMDTPMIRRFLAGYTAGAAFINPALRAPGRVVS
                     AFVARDASGFDDPARGAEMASMLLRVNSKVIFHAAGASGRGVFEAAGRFDAKAIGVDS
                     DQAAILASSGSAADAQLSKLVVTSMLKRVDRAVYALGQELVAGVRIPGGYRTFGLAEG
                     GVGYVAAGLPADAVTTLSDLARRVTSGEIVVPSNDQALADFVAVLR"
     gene            complement(2033..2704)
                     /gene="deoC"
                     /locus_tag="PLQ29_07095"
     CDS             complement(2033..2704)
                     /gene="deoC"
                     /locus_tag="PLQ29_07095"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPG86451.1"
                     /translation="MSKEWTKATIAKTIDHTLLKAIATEQQVRELCVEAKANGFASVC
                     VNPRWVPLCAKELQNSKVLVCTVIGFPLGANATETKAAEAALAVKQGAEEVDMVINLG
                     AAKAGDWKEVEEDIRMVVKAAGKATVKVIIETCYLTDPEKVKACEAAARAGAHFVKTS
                     TGFGTGGASADDVRLMKKAVGDRLKVKASGGIRSYHDAIQMLDAGADRLGASSGVAIV
                     SELPE"
     gene            complement(2719..3273)
                     /locus_tag="PLQ29_07100"
     CDS             complement(2719..3273)
                     /locus_tag="PLQ29_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86452.1"
                     /translation="MAEEEMIRLKSMHLAPYIQLATALIGKQRRSGGNMFRHQIDTFA
                     ILMDYGYIDSVLLKAAVIHDLVEDVPDFDRERILEIDEESQEVYQLVLEVTRRAVENK
                     QEFLARILEYGSPRALVLKSADRISNMISLGYTTDTAFVRRYCDETERFVFPIADKAN
                     TTMLAELVELVATRRDYLARCFEI"
     gene            complement(3339..>3694)
                     /locus_tag="PLQ29_07105"
     CDS             complement(3339..>3694)
                     /locus_tag="PLQ29_07105"
                     /inference="COORDINATES: protein motif:HMM:NF021941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPG86453.1"
                     /translation="FDGGEFIRRNAGLAAMLAGASGIEYVSAKAAGALALAGRDFEVY
                     VYAREAVDVAQLAARFSKEAQKERQYAERTRAKLANEAFVSSAPADIVEKERQKLREA
                     EIRAAKLEKYLADLA"
BASE COUNT          596 a         1208 c         1213 g          677 t
ORIGIN      
        1 ctttgatcat gccgttgccg gtgccgccag cggcgtggaa gatgatgtag gcgccggcct
       61 tgtactgctt ctgagccagg gcctgtccct tgtcgggagc gccgaaatcg ccggcgtagt
      121 caaccaggat ggtggcttcg gggtacacgg ccttgacgcc ctgctcgaaa ccggcctgga
      181 acttctcgat gagcgggaac tgcatgccgc cgatgaaacc gacgaccttc ttgccgtcgg
      241 ccttggcctt gaggccggtc gcgacgccga cgaggtagga accctcgtgc tcggcgaaga
      301 cggcgcagac cacgttcggc ttctgcacgc tggagtcgtc gatgatcatc agcttctgct
      361 gcggatactt ctcggcgacc tcgcccatgg cgttggcgaa gaggaagccg ggcgcgacga
      421 tcaggtcgag cttctcgtcg gcgaaggtgc tgaggttcgg cacgtagtcg gcctcggcgc
      481 cggactggag atacttatag tcggttccct tgacgagacc attctcctcg ccgaaacgga
      541 tgacgccttc ccaggtcccc tggttgaagg acttgtcatc gataccgccg agatcggtta
      601 cgaggccgac cttgaacacc ggcttggcgg cgaccggagc ggccttctct tccttggcgc
      661 aaccggtgaa ggcgagcgcg gcgatgagaa gcacggcgaa gaaagttcga tacttcatac
      721 taactccttt actttttcgc tatcgcgaaa atgtggaact cgatagtaac cccttacgcg
      781 attcagtgca agtcggtatt acaccttttt gagcgtatcg cgagcgccgt atcagcgcaa
      841 tacggccacg aaatcggcca aggcctggtc gttcgagggc acgacgatct cgccggaggt
      901 gacgcgcctg gccaggtcgg agagcgtcgt cacggcgtcc gccggtaggc cggcggcgac
      961 gtagccgact ccgccctcgg cgagtccgaa ggtgcgatag ccgcccggga tcctgacgcc
     1021 ggcgacgagc tcctggccga gcgcgtagac ggccctatcg acccgcttga gcatcgaggt
     1081 gacgacgagc ttggacaact gggcgtccgc ggctgagccg gacgaggcca ggatggccgc
     1141 ctgatccgaa tcgacgccga tggccttggc gtcgaagcgg ccggccgcct cgaacacgcc
     1201 gcggcccgag gcgccggcgg cgtggaagat caccttcgag ttgacgcgca acagcatgga
     1261 agccatctcg gcgccgcggg ccggatcgtc gaagccgctc gcgtccctgg ccacgaacgc
     1321 cgagacgacg cggcctggag ccctgagggc cgggttgata aaagcggccc cggccgtgta
     1381 gccggccagg aagcgccgta tcatcggcgt gtccatgcct ccgacgaagc cgacgcgggc
     1441 gtcgggcccg gccgcggcgg ccaggagacc ggcgtaggcc ccgaccagga acgagccctc
     1501 gtgctcggcg aagtcgacgc aggtcacgct cgaggaggcg ttcagatcgg gcacggtgcc
     1561 gtcgatgatg acgaaatgcg tttcaggata atcgctggcg accgagccga tggaatcggc
     1621 gaacaggaag cctacggcta tcaccaggtc ccggccgtcc tcggccaggc tgcgcaggag
     1681 ctgctcgcgg tcgacgccgc catgctttga ctcgagcacc cggatgtcga gcgcgtcgcc
     1741 gaagtcggcg tccgggtcgc cctcgatgcg gccgccggag tcccgggcca gcatcgcgag
     1801 gccgtcgtag gccgaacggt tgaacgatgc gtcgtcgcgg ccgccgatgt cgaagaccat
     1861 gcctatcgtc ggtttggctc gcttggcggt ggcgacggcg cgatcgttac cgccgcagga
     1921 cgacaatgcc agggtcaggg ccagcgcgat ggctacgaga cggcgatgct tcatggcgtt
     1981 cctccgcgga cagtataacc cgtttcaggc cgtagctggc gactaaagcg acctactcgg
     2041 gcagctcgct gacgatggcc acgcccgacg acgcgccgag ccgatcggca ccggcgtcga
     2101 gcatctggat ggcgtcgtgg tagctgcgga tgcctcccga ggccttgacc ttgagccggt
     2161 cgccgacggc cttcttcatg agccgcacgt cgtcggcgct ggccccgccg gtgccgaagc
     2221 cggtgctcgt cttgacgaag tgcgccccgg ccctggccgc cgcctcgcag gccttgacct
     2281 tctccgggtc ggtcaggtag caggtctcga tgatcacctt gaccgtcgct ttgccggccg
     2341 ccttgaccac catgcggatg tcctcctcga cctctttcca gtcgcccgcc ttggccgccc
     2401 cgaggttgat gaccatgtcg acctcctcgg cgccctgctt gacggcgagg gcggcctcgg
     2461 cggccttggt ctcggtggcg ttcgcgccca gcggaaagcc tatcacggta cagacgagca
     2521 ccttcgagtt ctggagctcc ttggcgcaga gcggcaccca gcgcgggttg acgcagacgc
     2581 tggcgaagcc gttggccttg gcctcgacgc acagctcgcg tacctgctgc tcggtggcta
     2641 tcgccttcaa gagggtgtga tcgatcgtct tggcgatggt cgccttggtc cattccttgc
     2701 tcatgtcgtc tccttggctt atatctcgaa gcatcgcgcc aggtagtccc ggcgggtggc
     2761 gacgagctcg acgagctccg ccagcatggt tgtattggcc ttatcggcta tcgggaacac
     2821 gaaacgctcc gtctcgtcgc aataccgacg cacgaaggcc gtatcggtgg tgtagcccag
     2881 cgatatcatg ttgctgatcc tgtcggcgct cttgagtacg agagcccgcg gactaccgta
     2941 ctccaggata cgcgccagga attcctgctt gttctcgacc gcgcgcctgg tcacctcgag
     3001 cacgagctgg tagacttcct ggctctcctc gtctatctcg aggatgcgct cgcggtcgaa
     3061 atcgggtacg tcctcgacga ggtcgtggat gacggccgcc ttgagtagca ccgagtcgat
     3121 gtagccgtaa tccatcagga tggcgaaggt gtctatctgg tggcggaaca tgttgccgcc
     3181 agagcggcgc tgcttaccta tgagcgcggt ggccagctgg atatagggcg ccaggtgcat
     3241 gctcttgagg cgtatcatct cctcctcggc catcgtcgcg gcccgcggag gcttcgccgc
     3301 ggggcgaagt ggccgcccct tcctatccat ccgacctcct acgccaagtc cgccaggtac
     3361 ttctcgagct tcgcggcccg tatctccgcc tccctaagct tctggcgctc cttctccacg
     3421 atatcggctg gagcgctcga tacgaaggcc tcgttggcca gcttggcccg ggtacgctcg
     3481 gcatattggc gctccttctg ggcctccttc gagaaccgcg ccgccaactg ggcgacgtcg
     3541 accgcttccc gggcgtacac gtatacctcg aagtcccggc cggccagggc gagcgcgccg
     3601 gcggccttgg ccgacacata ctcgatgccc gaggcgccgg cgagcatggc cgcgagcccg
     3661 gcgttgcggc gtatgaactc gccgccgtcg aagc
//
LOCUS       DAOPHV010000232         3689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_34348_length_3689_cov_3.320308, whole genome shotgun sequence.
ACCESSION   DAOPHV010000232 DAOPHV010000000
VERSION     DAOPHV010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34348_length_3689_cov_3.320308"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PLQ29_07110"
     CDS             complement(<1..593)
                     /locus_tag="PLQ29_07110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG86454.1"
                     /translation="MSDHTRLSPIRVLKRSMAPYLGLPRSVYTLFIATVVNGAGIFVF
                     PFMALILTRRFGLSEKETGDIVFLTTVAYVPGTLLGGRLADRFGRKRVQMASQLLCGA
                     LFIPCGFLDDPYLIAGFIIASVFFDGVTDPARTAMTMDVTTPENRQGAFSLIYLGHNL
                     GFAIGQIIAGFLFASAPSWMFWGNAMAVGASLVLVGL"
     gene            682..1668
                     /locus_tag="PLQ29_07115"
     CDS             682..1668
                     /locus_tag="PLQ29_07115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG86455.1"
                     /translation="MKYIAALIVVEDIARSRRFYEGLLGQVVESDYGEDVAFKGGFSI
                     HLKEHFSALLDGRAIGSRANDAELFFEHDDVAGLARRLAAAGIEFIHGPREQPWRQFC
                     ARFYDPDGHIVEVGERLQHLAWRLSEEGLGLDEICRVTYLSRPAVLRALAEYATGLFG
                     VVIESERLLQVAIRHDFDADIFREFTPAVATYMYPAPTCDRADTRAFVDSSIEGLQTG
                     EQLQLVIVDKERGEFIGCSGLHGLGRSDPELGIWTKLSAHGQGYGREAVTAIVAWARS
                     NASFERLVYPVDKRNVASRRIPESLDGVIAREFRQLNQAGFELDEVEYWITR"
     gene            1770..3017
                     /locus_tag="PLQ29_07120"
     CDS             1770..3017
                     /locus_tag="PLQ29_07120"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG86456.1"
                     /translation="MERNWKRDFSVFMGSQTLSLFGSMLVQYAITWHITMTTQSGSMM
                     TISIICAVLPQFFVSPFGGVLADRFDRKKLIAFADAGIALTTLALAASFAAGYDALWL
                     MFAAQAVRAVGAGIQTPAVGAMIPQIVPKDSLTRANAAFGSVQSVIMLIAPMAAGALM
                     SLAPIEALFMIDVVTAAIAIALLLGFLRLPTRAKATVPAGKLEVGYWMDLRDGFAYVG
                     AHTFVKRFLVFCAVFNFAAAPVACLTPLQVTRSFGNDVWRLTAIEIVFSVGMTLGGVV
                     MAAWGGFGNRVKTMALSSFIVGVTTLALGLVPSFWVYLAIMGLCGVAMPIFNVPASVL
                     LQETVEEAYMGRVFGVLGMISSVMFPLGMLVFGPVADVVPIEWLLVGTGAAIFAMGFF
                     LLASKALIAAGEGRGGRGETTAV"
     gene            3413..>3689
                     /locus_tag="PLQ29_07125"
     CDS             3413..>3689
                     /locus_tag="PLQ29_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86457.1"
                     /translation="MMLALERLARYNAVANQAMMDAMSLSGPGIFDEPVKGYYHSVHE
                     ILAHLYMADQLVLSELAASIGKERCGDEAAVPNDRPIVDLLQFRAARS"
BASE COUNT          653 a         1185 c         1138 g          713 t
ORIGIN      
        1 aacaggccga cgagcacgag ggacgccccg acggccatcg cgttgcccca gaacatccat
       61 gacggcgccg acgcgaatag aaatccggcg atgatctggc ctatcgcgaa gcccaggtta
      121 tggccgaggt agatcaggct gaacgcgcct tgccggttct cgggcgtcgt cacgtccatg
      181 gtcatcgccg tgcgcgccgg gtcggtgacg ccgtcgaaga ataccgacgc gatgatgaag
      241 ccggcgatca ggtacgggtc gtcgaggaag ccgcagggaa tgaacagggc cccgcagagc
      301 agctgcgacg ccatctgcac gcgcttccgg ccaaagcggt cggccagcct gccgccgagt
      361 agcgtgcccg gtacgtaggc gacggtcgtc aggaatacga tgtcgccagt ctccttctcc
      421 gacagcccaa agcgccgcgt cagtatgagc gccatgaacg ggaacacgaa gatgccggcg
      481 ccgttgacga ccgtcgcgat aaataacgta tagacgctcc gcggcagccc caggtagggt
      541 gccatggatc gcttgaggac gcgtatcggg gataatcgcg tatggtcgct catatgatcg
      601 actatacccg cccgggcgcg gcgggaatag ggatagttcc gctatactcg acctgctata
      661 ctccgatcgc gaggagtcga catgaagtat atagccgcgc ttatcgtcgt cgaagacatc
      721 gcccgctcac ggcgtttcta cgaggggctc ctgggccagg tcgtagaatc cgattacggc
      781 gaggacgtcg cgttcaaggg cggcttctcc atacacctga aggaacactt ctcggcgcta
      841 ctcgacgggc gcgccatcgg ctcccgggcc aacgacgcgg agctgttctt cgagcatgac
      901 gacgttgccg gcctggcccg tcgcctcgcc gcggccggca tcgagttcat ccacggcccg
      961 cgcgaacagc cctggcgtca gttctgcgcg cgtttctacg accccgacgg ccacatcgtc
     1021 gaagtgggcg agcgcctgca acacctcgcc tggcgcctgt ccgaggaagg cctcggcctc
     1081 gacgagatct gccgcgtcac ctacctgtcc cgacccgccg tactgcgggc cctcgccgag
     1141 tatgcaacgg ggctcttcgg cgtcgtcatc gagtcggagc ggcttcttca ggtggccatt
     1201 cgccacgact tcgacgccga catcttcagg gagttcaccc cagcggtcgc gacctatatg
     1261 tatccggccc cgacctgcga tagagccgat acccgcgcct tcgtcgactc gtcgatcgaa
     1321 gggctccaga ccggggaaca gctacagctc gtcatcgtcg ataaggaacg cggcgagttt
     1381 atcggctgct ctggcctcca cgggctgggg cggtccgatc cggagctcgg gatatggacc
     1441 aagctatcgg ctcatgggca gggctacggg cgtgaggcag tgacggccat cgtcgcttgg
     1501 gcccgctcga acgcgtcgtt cgagcgcctc gtctaccctg tggacaagcg caacgtagcc
     1561 agccggcgga tccccgagtc cctcgacgga gtcatagcgc gcgagttcag gcagctcaac
     1621 caagctggct tcgaactcga cgaggtggaa tactggataa cgcgctagcc gatccgccga
     1681 tagaacgctt tagctggaat ttcccttctc tccgcttcct ggttactata tcctcatcca
     1741 aaatgatacg ctaggaaacg gagcataacg tggaacgtaa ctggaagagg gacttttcgg
     1801 ttttcatggg cagccagacg ctgtcgctgt tcggctcgat gctcgtgcag tacgcgatca
     1861 cctggcacat cacgatgacc acgcagtccg gttcgatgat gacgatatcc atcatctgcg
     1921 ccgtgctgcc tcagttcttc gtctcgccct tcggcggcgt cctggccgac cgtttcgacc
     1981 gcaagaagct gatagccttc gctgacgcgg gaatagcgct gacgaccctg gccctcgcag
     2041 cgtcgttcgc ggctgggtac gacgccctgt ggctgatgtt cgcggcgcag gccgtccgcg
     2101 cggtcggcgc cgggatccag acgccggccg tcggcgcgat gatcccgcag atcgtgccca
     2161 aggatagcct cacgagggcg aacgccgcct tcggtagcgt ccagtcggtg atcatgctta
     2221 tcgccccgat ggccgccggc gcattgatgt cgctcgcccc gatcgaggcg ctgttcatga
     2281 tcgatgtcgt cacggcggcg atcgcgatag cattgctcct cggctttctg cgcctaccga
     2341 cacgcgcgaa ggcgacggtg ccagcgggta agcttgaggt cggctactgg atggacctgc
     2401 gcgacggctt cgcctacgtc ggcgcccaca ctttcgtcaa gcgcttcttg gtcttctgcg
     2461 ccgtcttcaa tttcgccgcg gctccggtcg cctgcctgac gcctctgcag gtcacgcgta
     2521 gcttcggcaa cgacgtctgg cggttgaccg ccatagagat cgtgttctcg gtcggaatga
     2581 ccctcggcgg cgtcgtgatg gccgcctggg gtggcttcgg caatcgcgtg aagaccatgg
     2641 ccttgtcatc gttcatcgtt ggcgtcacga ccttggccct gggcctggtg ccatcgttct
     2701 gggtctacct tgcgattatg gggctgtgcg gcgtcgccat gcccatcttc aacgtacccg
     2761 cctcggtgct cctgcaggaa accgtcgagg aagcgtacat gggccgcgtc ttcggcgtgc
     2821 tcggcatgat atcgagcgtg atgttccctt taggcatgct ggtcttcggc cccgtggccg
     2881 acgtcgtacc gatagaatgg ctcctcgtcg gcacgggagc cgcgatcttc gccatgggat
     2941 tcttcctgct cgcgagcaag gctctgatcg ccgccggcga aggacgaggc gggcgtggcg
     3001 agacgaccgc ggtgtgaaaa aagcggggat ggctcccgcc gatccccgca ttgtacgaca
     3061 tacaacccgg accatccggc cagggtttac tggtcgagcg cgaacccccg ataggtcgga
     3121 atcggcgcct tcgcttccgc ctatgtctcc gcgttctcgc tatcctggac gcgctgggcc
     3181 gatatgtcgt gttggtgacg gcccgagccg tagacgctga taacggacgg agccgagctg
     3241 atcggatcta tcatcgcgtt accctcccgt acctataagc ttatccatcc gccagacgaa
     3301 atcaagggca cggggccgat tatcttcatc gtgcgtcagc tgttttattg atatactatc
     3361 acatctcaaa tcggacggcc gatcaccgca gtcggcccgc aaggagtctc gtatgatgct
     3421 cgccctcgag cgcttggccc gctataacgc cgtcgccaac caggctatga tggacgcgat
     3481 gagcctatcc ggccccggga tcttcgacga gcccgtcaaa gggtactacc atagtgtgca
     3541 cgagatactc gcccatctct atatggccga ccagctcgtc ctatcggagc tagcagcgtc
     3601 gataggcaag gaacgatgcg gagacgaggc cgccgtcccg aacgacaggc ctatcgtcga
     3661 cctgcttcag ttccgcgcgg cccgctcgg
//
LOCUS       DAOPHV010000233         3687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_34367_length_3687_cov_5.557544, whole genome shotgun sequence.
ACCESSION   DAOPHV010000233 DAOPHV010000000
VERSION     DAOPHV010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34367_length_3687_cov_5.557544"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="PLQ29_07130"
     CDS             <1..959
                     /locus_tag="PLQ29_07130"
                     /inference="COORDINATES: protein motif:HMM:NF020071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HPG86458.1"
                     /translation="ESVGELLAPGFPDRLARRLADDSWEFPSGRRARSAYAPPRVGWL
                     LALDADAGDPLGRIRRAAPVGERAARAAMERGSSVSIELEWRGLSASAWERRRFGVFA
                     LTERRLERAPRELLEAGFAERLRVEGLAWLPWNDEARSIVDRARYVAARAGAAYGVDA
                     AVWTDEAVSDALAAGAVDWLSDTGPVIDGASLVAMLAGRLGPDGVRGLDRAAPPFVET
                     PGGRKRRPTYPVAGPARLAGRIQEFFGMEDGPRACGEPMTLELLSPADRPLQVTSDLA
                     GFWRNTYPSIRAEMARRYPRHYWPTDPLAAEPTRGQRPRPSA"
     gene            complement(949..3066)
                     /locus_tag="PLQ29_07135"
     CDS             complement(949..3066)
                     /locus_tag="PLQ29_07135"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPG86459.1"
                     /translation="MKRYLVALVCAMTALAAHAQFVDLGESMLYVRVGFEDSWTRGLP
                     RLDADGWTVLEPSGGKRALIMRDLGLPGSPEGGVLSLFRREPIEYTVLAPFQADLTLL
                     NSSDPALFLKHVGQSWAVYLNGVLLRNEFVSYGEGYKERSLVDVVVPLDKRLLTRGSN
                     LLAFRIRGDPVDDRTGFNQPGPYVIDSYRSLVSRNGEYVDLMLIGVYAFFALYHGVLF
                     ALRPKDRGYLYFSASALLMALYMWSRSAIATELVLDTALLRYVEYASLFMLLPAVVAF
                     IRTVLDDRPFRMPAILAAASAVLALACLFLRLEAMLYAWYLVAAVGSVYAFGVVIRKA
                     VLKDDGADSGLARLVLSRGESVGIIAGVLAFAAASAIDVWVVVSSGGNVVWSKYAFFL
                     FSLTMSATLAGRFTKVAARAEAMNADLEAEVASRTSELSAAAAERLRLNQELSSANAR
                     LGASMEEAERDLRIASSVQKGFFPSRAPEVSDWDVAFAYEPVSGVSGDFYDFYSRDGS
                     LSGAVLGTVSGAGIASGLVTVLAKNVFSRGMLELSEEPLSAMLVEVNRRMIRELSAVG
                     NTVSCAFMRFAGDRVEYVNAAHTDALLRRAGKRAVSFVKAKQGPSKAPPLGRDDFEAR
                     VGALAFSAKPGDALLVYTAGLVNGANAKGAPYGVERLSEAFARADPDSAESTLASLMI
                     DYRNYLAGSRRGGDVGVMVFVKR"
     gene            3196..3504
                     /locus_tag="PLQ29_07140"
     CDS             3196..3504
                     /locus_tag="PLQ29_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86460.1"
                     /translation="MKGTIKTIATLAVIGAALYFTNPSMEDFGRYYERKLVAESQQGT
                     ADPLKKIAKALAQTGADLAIKAGAKRQDKLLFSVFTLGPASKPAHRYLGLAKFVFVEL
                     "
BASE COUNT          620 a         1233 c         1288 g          546 t
ORIGIN      
        1 tggagtccgt gggcgagctc ctggcacccg gcttccctga ccgcctagcg cggcgcctgg
       61 ccgacgacag ctgggagttc ccgtccggac ggcgggccag atccgcctac gctccgccga
      121 gggtcggctg gctcctggcc ttggacgccg atgccggaga cccgctcggc cggatacgcc
      181 gagccgcgcc ggtgggggaa cgggcagcca gggccgcgat ggaacgaggc tcgtcggtat
      241 cgatcgagct cgaatggcgc gggctttcgg cctcggcctg ggagcggcgc cgcttcggcg
      301 tcttcgcgct gaccgagcgc cgcctcgagc gcgcgcccag ggagctgcta gaagccggct
      361 tcgccgagcg tctgcgcgtg gagggcctag cctggttgcc gtggaacgac gaggcgcgat
      421 ccatcgtcga ccgagcgcgc tacgtcgccg ccagggccgg tgccgcttac ggcgtggacg
      481 cggccgtctg gaccgacgag gccgtctccg acgccctggc ggccggcgcc gtggactggc
      541 tatcggatac cgggccggtc atcgacggcg cgtccctcgt cgccatgctc gccgggaggc
      601 tcggcccgga cggcgtccga gggctcgacc gagccgcgcc gcccttcgtc gagacgcccg
      661 gcggcaggaa gcggcgcccg acctacccgg ttgccggtcc ggccaggctg gccggccgta
      721 tccaggagtt cttcgggatg gaggatgggc cgagggcctg cggggagcct atgaccctcg
      781 agctcctgtc gccggcggac aggccgctcc aggtgacgag cgacctggcc ggattctgga
      841 ggaacaccta tccgtcgata cgcgccgaga tggccaggcg ctatcccagg cactactggc
      901 cgaccgatcc gctcgccgcc gagccgacca ggggccagcg gcctcggcct agcgcttgac
      961 gaagaccatc acgccgacgt cgccgccgcg cctcgagccg gccaggtagt tccggtagtc
     1021 tatcatcaag gaggcgagcg tcgactcggc gctgtcgggg tcggccctgg cgaaggcctc
     1081 gctgaggcgc tcgacgccgt agggcgctcc cttggcattg gcgccgttga cgagcccggc
     1141 cgtatagacg aggagcgcgt cgcctggctt ggcgctgaag gcgagagcgc cgaccctggc
     1201 ctcgaagtcg tcgcggccga gcggcggagc cttggacgga ccctgcttgg ccttgacgaa
     1261 ggagaccgcc cgtttgccgg cccgcctgag cagcgcgtcg gtatgggccg cgttgacgta
     1321 ctcgacgcgg tcgccggcga agcgcatgaa ggcgcaggag acggtgttac cgaccgccga
     1381 cagctcgcgg atcatgcgcc ggttcacctc gacgagcata gccgacagcg gttcttccga
     1441 tagctcgagc atgcctcggc tgaacacgtt cttggcgagc accgtgacga ggccgctggc
     1501 gatgcccgcg cctgacaccg tgccgagcac cgcgccgctc agcgagccgt cgcgcgagta
     1561 gaaatcgtag aagtcgccgg atacgcccga gaccggctcg taggcgaacg ctacgtccca
     1621 gtccgagacc tccggagccc ggctcgggaa aaagcccttc tgtaccgagg aggcgatgcg
     1681 caggtctcgc tcggcctcct ccatcgacgc tcccagcctg gcgttcgccg acgacagctc
     1741 ctggttgagc ctgagccgtt cggcggcggc ggctgacagc tccgaggtcc tcgaggcgac
     1801 ctcggcctcc aggtcggcgt tcatcgcctc ggcgcgggcg gctaccttgg tgaagcggcc
     1861 ggccagggtg gccgacatgg tcagggagaa taggaagaac gcgtacttcg accagacgac
     1921 gttgccgccc gaggagacga cgacccagac gtcgatcgcg gaggccgcgg cgaacgcgag
     1981 cacgcccgcg atgatcccga cagactcgcc gcgggacagt acgagcctgg ccaatccgct
     2041 atccgccccg tcgtccttaa gcacggcctt cctgatgacg acgccgaacg cgtagaccga
     2101 cccgacggcg gcgaccaggt accaggcgta gagcatcgcc tcgaggcgca ggaagaggca
     2161 ggctagcgcc aggacggccg acgccgcggc gagtatcgcc ggcatcctga agggccggtc
     2221 gtccaggacg gtccggatga acgccaccac cgccggcagc aacatgaaca gggaggcgta
     2281 ctcgacgtaa cggagcaagg ccgtatccag taccagctcg gtcgctatcg cgctcctcga
     2341 ccacatgtag agcgccatca gcagggccga ggcgctgaag tacaggtagc ccctgtcctt
     2401 aggcctgagc gcgaacagga cgccgtggta caacgcgaag aaggcgtaga cgcctatgag
     2461 catcaggtcg acgtactcgc cgttacgcga gacgagcgac cgatacgagt cgatcacgta
     2521 gggccccggc tggttgaagc cggtcctgtc gtcgacaggg tcgccgcgga tcctgaaggc
     2581 gagcaggttg gagcctcgcg tcagcaggcg cttatccagg ggcacgacca cgtctaccag
     2641 ggatcgctcc ttatagcctt cgccgtagct cacgaattcg ttgcgcaaca gtacgccgtt
     2701 caggtagacg gcccacgact gcccgacgtg cttgaggaac agggccgggt cgctggagtt
     2761 gagcagggtg agatcggcct ggaagggcgc gagtaccgtg tactcgatcg gctcgcggcg
     2821 gaagaggctg aggacgccgc cctcggggga gccgggcagg ccgagatcgc gcatgatcag
     2881 ggcgcgctta ccgccgctcg gctcaagcac ggtccagccg tcggcgtcga gcctgggcag
     2941 gcccctggtc caggagtcct cgaacccgac gcgaacgtag agcatggact cgcccaggtc
     3001 gacgaattgg gcgtgggccg ccagggccgt catggcgcat accagcgcga cgaggtatcg
     3061 tttcatagcg ttaccacatc ctacggcccc atcccgcccg ggtcaagccg gaatacgcgc
     3121 ggagtccggc ttgcggaacg cccggcgccg ggctattgtt catggtctcc ggcgtatatt
     3181 tagaaaggaa acggcatgaa aggcaccatc aagaccatcg cgaccctggc cgtcatcggg
     3241 gcggcgctct acttcaccaa tccgtcgatg gaagacttcg gcaggtacta cgagaggaag
     3301 ctggtggccg aaagccagca gggtaccgcc gatccgctta agaagattgc caaggcgctg
     3361 gcgcagaccg gcgcggacct ggcgatcaaa gccggcgcca agcggcagga caagctgctg
     3421 ttcagcgtct tcacgctcgg cccggcgtcc aagccggcgc atcgctatct gggcctggcc
     3481 aagttcgtct tcgtggagct gtaagtcccg gggccatggg tatattctcc ttcctgcgcc
     3541 gcaggaggcc gggagactat gacgagctac gcatcgagct gtgggacgcc gtccgcgccg
     3601 gcaggccggc cttcgcgtcg cttacgccgg aagactgctc ggctctccga cgcatggctt
     3661 cgtggttcat cgacgtcaag gatttcg
//
LOCUS       DAOPHV010000234         3682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_34431_length_3682_cov_3.918114, whole genome shotgun sequence.
ACCESSION   DAOPHV010000234 DAOPHV010000000
VERSION     DAOPHV010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34431_length_3682_cov_3.918114"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..528
                     /locus_tag="PLQ29_07145"
     CDS             157..528
                     /locus_tag="PLQ29_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86461.1"
                     /translation="MDVRISNQLPLAYAYSAGASGRISVPVSPSQLLYSNFKNVSGIA
                     SAGASAYTLDKLKILDTIIERLRSAKNQPRVEREATGLSDERIDALIEQYGAQLHNAL
                     GAGPYAKPTGVVPGMLVSIAA"
     gene            complement(690..3044)
                     /locus_tag="PLQ29_07150"
     CDS             complement(690..3044)
                     /locus_tag="PLQ29_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPG86462.1"
                     /translation="MRSFARVVAIALSLSIQSCAGDSIHGLDEAGWFELLAKGDPLPI
                     AYGDRVDYDAIARLGPGASLFVGLRALEAGDEKLAAGLFRDAAARETGRYKARAVASL
                     VETLQSTGDGEALVELCRSELGSELAPYRRAYLEAVGYAVSGSHAHTLASVDALRSAY
                     PVESSRDAAAIASMALEAGYRSGKGRWVNDFIALTAMEGSAAIYEALAKATSLVASSG
                     RAEAEAAIRAVGVRAFQLAEARALTGSRNYGPAVVAFRRYAADREDPADVAVRVASAP
                     PPDAEAAPSIPLSPKAAANLFLTLPRASASDAAKAFIAAAKDEGGEGFRYIVETIADR
                     GADPARAYFDAFWRGRFLRADERWAEAERWFAKAAALAENRWDLDAARWYEIEAASKR
                     SAAAAVGLLGAALESSSNPGYYSDLVEPLSREALVARSGSTLAAIDAATARAAPKDRA
                     RLSYLCARAAQAGIIRADHVASAFGGSFVDLGGYIDSRLHEAYGQRVDPWYRLVAAYR
                     LGEPLVEPLAVPGTEPAPTESGGTASADDEARRPVSPDEYALALTRFGLGYRVRAELG
                     DEFRSVSENAVRTIAAELDAAGRHDQAYRLIATLFWRTGFEPTRSDAELYWPRPYPEA
                     FSPAIEATGLDPHLLYGLARSESAFDPKAVSKSGAVGLIQLMPATAAETAERLDLDDY
                     ELTDPLDNVTIGSSYLARLLDRLAGRVLPAVFSYNGGPTRFRRWEAEYGALPLDLLLE
                     ALSYAETRQYGRNVGHAALSYAALYGEVDLRAYMAWLLGEGPRP"
     gene            complement(3186..>3682)
                     /locus_tag="PLQ29_07155"
     CDS             complement(3186..>3682)
                     /locus_tag="PLQ29_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPG86463.1"
                     /translation="DDRESTAEEPDRPRRASPLLIGILAALATALILAAAFFIYRCTA
                     DRQAEPPQAASAPAQAPTPAPAPAPTPAPTPTPTPAPTPTPAPEPAKVETPAPVPAPA
                     PTPAAPAKKEGTWHKIKWGDTLWDISIAYYRTPWLYMTIAKANKIKNPDLIISGTWIY
                     IPPR"
BASE COUNT          595 a         1204 c         1303 g          580 t
ORIGIN      
        1 cgacgagggg ctcggccggg cggtcaacga ccggctgtac aaggcctcgg tcaagtaggg
       61 ccgtctatcg tcgccgttcg tcagctaacg ctaatactcg cccctcgccg ctccgacaat
      121 ataggtagaa agtcggtatc gagtacggag cggatcatgg acgtgaggat ttcgaatcag
      181 cttccattag cgtacgccta tagcgcgggg gcgagcggca ggatatccgt accggtctcc
      241 ccgtcacagc tcctgtactc caacttcaag aacgtatccg gcatcgcgag cgctggcgct
      301 tccgcctaca ccctggacaa gctcaagatc ctcgatacga tcatcgagcg cctgcgaagc
      361 gccaagaacc agcccagggt cgaacgcgag gccacgggcc tatccgacga acgcatcgac
      421 gcgctgatcg agcagtatgg ggctcagctc cacaacgccc tcggcgcggg accgtacgcc
      481 aagccgaccg gggtggtccc gggtatgctc gtctccatcg ccgcctagtt cctcatctcc
      541 gcctgagtgg cacgaagctt attggtatct cgatgcggta gatgaaacct gcccgaacgc
      601 cgaggcccga tcgctacaac tcggccgcgg cgagccggac aagcgctata cgtacataac
      661 ccacaagatt cgtaacacta aagctaggcc taggggcgcg gaccctcgcc gagtagccac
      721 gccatatagg cgcgcaggtc gacctctcca tagagcgcgg cgtaagacaa cgcggcgtga
      781 ccgacgttgc ggccgtattg cctggtctcc gcgtacgaca gcgcctcgag cagcaggtcg
      841 agcggtagcg ccccgtactc ggcttcccag cgcctgaagc gcgtcgggcc gccgttgtac
      901 gagaatacgg ccgggaggac cctaccggcc agccgatcga ggagccgggc taaataggac
      961 gagcctatcg tcacgttatc gagcgggtcg gtcagctcgt agtcgtccag gtccaggcgc
     1021 tcggcggtct cagcggcggt cgccggcatc agctggatga gcccgacggc cccggacttg
     1081 gatacggcct tcgggtcgaa ggcgctctcg gagcgcgcga gcccgtagag caggtggggg
     1141 tcgaggccgg tcgcctcgat cgccggactg aaggcctcgg ggtagggccg cggccagtac
     1201 agctcggcgt cgctgcgagt cggctcgaag ccggttctcc agaacagagt cgctataagt
     1261 cggtacgcct gatcgtgccg tccggcggcg tcgagctcgg cggctatcgt cctgaccgcg
     1321 ttctcggaca cggatcggaa ctcgtccccg agctcggccc ggacgcggta accgagcccg
     1381 aagcgagtca gggctagggc gtactcgtcc gggctgacgg ggcggcgcgc ctcgtcgtcc
     1441 gcagatgccg tcccgccact ctcggtgggg gccggctcgg ttccggggac ggcgagcggc
     1501 tcgacgaggg gctcgccgag tctgtaggcg gcgacgagtc ggtaccatgg gtcgacccgc
     1561 tggccgtacg cctcgtgtag cctcgagtcg atgtaaccgc caaggtcgac gaaggagccg
     1621 ccgaaggccg aggcgacgtg atccgcgcgg ataatgccag cttgcgcggc tcgggcgcag
     1681 aggtacgaca gcctggccct atccttcggc gcggcccgcg cggtcgcggc gtcgatggcg
     1741 gccagcgtcg agcctgacct ggcgacgagg gcctcccgcg atagcggctc gacgaggtcc
     1801 gagtagtagc cggggttaga cgagctttct agcgccgcgc cgagcagccc gacggccgcc
     1861 gcggccgacc gtttggaggc cgcctcgatc tcgtaccagc gcgccgcgtc caggtcccaa
     1921 cggttctccg ccagcgcggc ggctttggcg aaccaccgct cggcttcggc ccagcgctcg
     1981 tcggcacgca ggaagcgccc gcgccagaac gcgtcgaagt acgcgcgcgc ggggtcggct
     2041 ccgcggtcgg cgatcgtctc gacgatgtac ctgaagcctt cgccgccctc gtccttggct
     2101 gccgcgatga aggccttggc tgcgtccgag gccgaggcgc ggggcagggt caggaagagg
     2161 ttggcggcgg ccttcggcga cagcgggatc gacggcgcgg cctcggcgtc ggggggcggc
     2221 gcgcttgcga ccctgacggc cacgtcggcc gggtcttccc ggtcggcggc gtagcgcctg
     2281 aaggcgacga cagccggccc gtagttccgt gaaccggtca gcgcgcgggc ctcggccagt
     2341 tggaaggccc tgaccccgac ggcccgtatc gcggcctccg cttcggctcg gccggatgag
     2401 gccacgaggc tcgtggcctt ggcgagcgcc tcgtagatgg ccgccgatcc ttccatcgcc
     2461 gtgagcgcta tgaagtcgtt cacccagcgg cccttgccgg agcggtagcc cgcctcgagg
     2521 gccatcgacg cgatggccgc cgcgtcccgc gaggattcga cggggtaggc cgagcggagc
     2581 gcgtccacac tcgccagggt atgggcatgc gagcccgaga ctgcataccc gacggcctcc
     2641 aggtaggccc ttcggtacgg cgcgagttcc gagcccagct cggagcggca cagctccacc
     2701 agcgcttccc catcgcctgt cgattgcagg gtttcgacga gagaggcgac cgcccgcgcc
     2761 ttgtatcgcc cggtctctcg cgccgcggca tccctgaaca gcccggcggc gagcttctcg
     2821 tcgcccgctt cgagggcccg caggccgacg aacaagctgg ctccgggccc gagcctggcg
     2881 atggcgtcgt agtcgacgcg gtcgccgtag gcgatgggca gggggtcgcc tttcgctaag
     2941 agctcgaacc agcccgcctc gtcgaggccg tggatcgagt cgccggcgca tgactggatg
     3001 gataggctca gggctatggc gacgacgcgg gcgaatgatc gcatggactc tcctggcgag
     3061 gtgatggtgg cccgttggcg cgggcctcgc tactactatt ccacgaaagc ggccataggc
     3121 caagcgtcga gccttaaaaa aaacggcggc gcagatgcga ccgccgttat cgcccaggct
     3181 cgcgcctagc gcggcgggat gtagatccag gttcccgaaa tgatcaggtc ggggttcttg
     3241 atcttattgg ccttggctat cgtcatgtac agccatggag ttcggtagta ggcgatcgaa
     3301 atatcccaga gcgtgtcgcc ccacttgatc ttatgccaag taccctcttt cttagccggg
     3361 gcggcgggag taggagcggg agccggaacg ggagccggcg tctcgacctt ggccggttcg
     3421 ggcgctggag tcggcgtagg cgctggagtc ggcgtaggag tcggggccgg agtgggcgct
     3481 ggagccgggg ccggagtggg cgcctgagcg ggagccgaag cggcttgagg cggttcggcc
     3541 tggcgatccg cggtgcaacg atagatgaag aacgcggcgg cgaggatcag cgcggtggct
     3601 agcgccgcca gtataccgat gaggagcggc gaggcgcgcc tcggccggtc cggctcctcg
     3661 gcggtggact cgcgatcgtc ga
//
LOCUS       DAOPHV010000235         3678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_34480_length_3678_cov_4.691692, whole genome shotgun sequence.
ACCESSION   DAOPHV010000235 DAOPHV010000000
VERSION     DAOPHV010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34480_length_3678_cov_4.691692"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PLQ29_07160"
     CDS             complement(<1..499)
                     /locus_tag="PLQ29_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPG86464.1"
                     /translation="MTKSHYDELPSEIRRQLDSMIHDSGLSGGPDARERFAALWAEKF
                     RLFSSQIESLGMTMEASFAEGDARAAILLTYSGSLVTLGPRRGKDRWIEYASIKMRTD
                     VPDLVRGDGATQVGPIVRGETASFSGCPVKKTSAIYRIAVCPAGASADDQERRDDQER
                     RDDQCK"
     gene            657..1181
                     /locus_tag="PLQ29_07165"
     CDS             657..1181
                     /locus_tag="PLQ29_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86465.1"
                     /translation="MSAFDGLGTVIQGHIRQIVKTSGLRDGQESLEKLAEAWTMKREA
                     FEAATREHGLEEVSFFGAEEERGALALTYSGSIVTIGPLDAGKRRCEYASVGLRADVP
                     QSATEPESTLSADVETDGPIAFSRGPVKSTSPVFKIAVAAERLPLDEETTLLTQVAGD
                     VAEDFARVNRTVVL"
     gene            1187..2284
                     /locus_tag="PLQ29_07170"
     CDS             1187..2284
                     /locus_tag="PLQ29_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86466.1"
                     /translation="MTRGMAPSGPDEARERLRESFLRPGRPDRDRLRAVVEAAAGALA
                     AEPAERRPPASDGRPGGLVRLLSGVPTIVVPDLHARGAFILSLAAMPLPDGGDICGAL
                     AEGALQVLCLGDGFHAEARAAGRWRDAYREFLGGYTESTAMDDEMRESLSLMEMVMIL
                     KAAFPRAFHFLKGNHENVLDEEGDGNHSFGKFAEEGAMVAEYMLGAYGRELTESYAAF
                     EKALPLFAVGDRFLASHAEPARAYSDEELVDAPNRPEVILGLTWTDNGAAEPGAVGAL
                     LDRILPNVPDARYITGHRPVPAPLRYLERSDGRHLQIHAPDSFNVAWVMPDRALAPEL
                     DVGAIPDISSSLPRQAGGQRRTHGGATRIDR"
     gene            2256..>3678
                     /locus_tag="PLQ29_07175"
     CDS             2256..>3678
                     /locus_tag="PLQ29_07175"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPG86467.1"
                     /translation="MAELPASIGKYPIDALLARGGMGAVFKATHPTLKRHVVLKKLTM
                     RGSASIAERFKREARILMDFKHDNIVRVFDHFKEGSSHYMVMEFVDGESLDALIRRRR
                     FLSTELSLAIFLKACRALAYAHERGVVHRDIKPGNILISKKGEVKLADFGIASSGDED
                     DGGLTREGMTLGTPSYMPPEQIENAKNVDKRADVYAMGIMLYEMTTGKKPFPGSFSPE
                     TVNLIQKGRYRKPSSVNRSVKPIVDRLVRKLARPDPKRRYQDMSEVIRVVERFLARYQ
                     AAELDEALVGLMTGSLKDEPRYRPRRKKRLAWATVLLALAAACAGGWYAYREGYAYRW
                     LIPSRYGELRVSLRVPKAGLSPDGPSVRARLFVDDGDEMPEVTGAAIVFGLSEPAASD
                     PALAFESGPLYLAPGDYRLKLIVDGRVLWRSFRLRPISLEPSTALRLRLDEEPAKPLS
                     VRVAASDLTDGSDLSGRATVSLYR"
BASE COUNT          601 a         1254 c         1222 g          601 t
ORIGIN      
        1 gcttacactg gtcatcgcgg cgctcctggt catcgcggcg ctcctggtcg tcggcgctgg
       61 cgcccgccgg gcatacggct atccggtaga tcgccgaggt cttcttgacc ggacagccgg
      121 agaacgacgc cgtctcgccg cggacgatag ggcctacctg cgtagcgccg tcgcctcgca
      181 ccaggtcggg cacgtcggta cgcatcttga tcgacgcgta ctctatccag cgatccttgc
      241 cgcgccgcgg ccccagggtg acgagggagc cggaataggt caggagtata gccgcccgcg
      301 cgtcgccttc ggcgaaggat gcttccatcg tcatgccgag cgactcgatc tgggagctga
      361 acagcctgaa tttctcggcc cacagagccg cgaaacgctc cctggcgtcc ggcccacccg
      421 atagccccga gtcgtggatc atcgagtcca actgcctgcg tatctcgctc ggtagttcgt
      481 cgtaatggct cttcgtcata tcggcctccg taatgtagta taatccaacg tggattatat
      541 ttcatttttt cttggctttt tccgtgaaaa attcaaaagt gatagtacat tgcttatatg
      601 cgctgacgac gaggcggcaa tggagccgcc taagggtcag cgcgcgagga ggaactatga
      661 gcgcgttcga cggcctgggc accgtgatcc agggacacat caggcagatc gtcaagacct
      721 ccggcctccg ggacggccag gagagcctcg agaagctggc cgaggcctgg accatgaagc
      781 gggaagcctt cgaggctgcc acccgtgagc acggcctcga agaggtttcg ttcttcgggg
      841 ccgaagagga gcgtggcgcg ctggccctca cctactccgg ctcgatcgtg accatcgggc
      901 cgctcgatgc cggcaagcga cgctgcgagt acgcctcggt aggcctacgg gccgacgtgc
      961 cccagtcggc gaccgagccg gaatcgaccc tgtccgccga cgtcgagacc gacggcccca
     1021 tcgccttctc ccgcggcccg gtcaagtcga cctcgccagt cttcaagata gccgtggccg
     1081 cggaacgctt gccgctcgac gaggagacca cgttgctcac ccaggtggcc ggggacgtgg
     1141 ccgaggactt cgcccgcgtc aaccggaccg tggtactgta ggcgacatga cccgaggtat
     1201 ggcgccgtcc ggcccggacg aggcgcgcga gcgcctgcgc gaatcgttcc tgaggcccgg
     1261 ccgccccgac agggaccggc tccgcgccgt cgtcgaggcc gctgccggcg ccctggccgc
     1321 tgagcctgcc gagcgccggc cgccggcgtc ggacggccgg cccgggggtc tcgtacggct
     1381 cctgtcaggg gttccgacca tcgtggtgcc cgacctgcac gcgcgaggcg ccttcatcct
     1441 cagcctggcg gccatgcctc tgcccgatgg cggagacatc tgcggggcgc tggccgaggg
     1501 cgcgctccag gtgctatgcc tcggcgacgg cttccacgcg gaggccaggg cagcgggccg
     1561 ctggcgcgac gcgtaccgcg agtttttagg cggttacacg gaatcgacgg cgatggacga
     1621 cgagatgcgc gagagcctgt cgctgatgga gatggtcatg atcctcaagg cggccttccc
     1681 gcgggccttc cactttctca agggcaacca cgagaacgtg ctcgacgagg aaggtgacgg
     1741 caaccattcc ttcggcaagt tcgccgaaga gggcgcgatg gtcgccgagt acatgctcgg
     1801 ggcctacgga cgggagctga ccgagtcgta cgctgccttc gagaaggccc tgccgctgtt
     1861 cgcggtcggg gatcgcttcc tcgcctcgca cgccgagccg gcgcgcgcct actccgacga
     1921 ggagctcgtc gacgcgccga accggcccga ggtcatcctt ggcctgacct ggaccgacaa
     1981 cggggccgcc gagccgggcg ccgtgggcgc cctgctcgac cgtatcctgc cgaacgttcc
     2041 cgacgcccgc tacatcaccg gccaccggcc ggtgcctgcg ccgctacgct acctggagcg
     2101 ctcggacggg cgtcacctgc agatccacgc tcccgattcg ttcaacgtgg cgtgggtcat
     2161 gcccgaccgc gccctcgcgc ccgagctgga cgtgggcgcc ataccggata tttcgtcgag
     2221 cctcccgcgc caggcgggag gacagaggag gacgcatggc ggagctaccc gcatcgatcg
     2281 gtaagtaccc tatagacgcc ttgctcgcca ggggcggcat gggggccgtg ttcaaggcga
     2341 cccacccgac gctcaagcgc cacgtcgtcc tcaagaagct gaccatgcgc ggctccgcgt
     2401 ccatcgcgga gcgcttcaag cgcgaggcca ggatcctgat ggacttcaag cacgacaata
     2461 tcgtgcgagt cttcgaccac tttaaagaag gttcgtcgca ctacatggtg atggagttcg
     2521 tcgacggcga gtcgctcgac gcgctgatcc gccgtcgccg cttcctgtcg acggagctat
     2581 cgctggccat cttcctgaag gcttgccgag cgctcgccta tgcccacgag cgcggcgtcg
     2641 tccaccggga tatcaagccg ggcaacatcc tgatatcgaa gaagggcgag gtgaagctcg
     2701 ccgacttcgg catcgcctcc tcgggcgacg aggacgacgg cggcctaacc cgcgagggca
     2761 tgacgctcgg cacgccgtcg tacatgccgc cggagcagat cgagaacgcc aagaacgtcg
     2821 acaagcgggc cgacgtctac gccatgggca tcatgctcta cgagatgact accggcaaga
     2881 agcccttccc cggtagcttc agccccgaga cggtcaacct gatccagaaa ggccgctacc
     2941 gtaagccgag ctccgtcaat cgctcggtca agccgatcgt cgaccgcctg gtgcgcaagc
     3001 tggccaggcc cgatcccaag cggcgctacc aggacatgtc cgaggtcata cgcgtggtgg
     3061 agcgcttcct ggcacgctac caggcggccg agctcgacga ggcgctcgtc gggctgatga
     3121 ccggttcgct gaaggatgag ccccgttacc ggccgcggcg caagaagcgg ctcgcctggg
     3181 cgacggtcct cctagcgctg gccgcggcct gcgccggggg ctggtacgcc taccgcgagg
     3241 gctacgccta ccgctggctc attccgtcgc ggtacggcga gttgcgcgtc tcgctgcgcg
     3301 tcccgaaggc cggcctctcg cccgacggcc cgtcggtgcg ggcgcgcctg ttcgtcgacg
     3361 acggcgacga gatgcccgaa gtgaccggcg cggccatcgt cttcggcctc tccgagccgg
     3421 cggcgtcaga cccggcccta gccttcgagt cggggccgtt gtacctggct ccgggcgact
     3481 accgcctcaa gctgatcgtc gacgggcggg tgctctggcg ctcgttcagg ctgaggccga
     3541 tatccttaga gccgtcgacg gcgctcaggc tcaggctcga cgaggagccg gccaagccgc
     3601 tatccgtcag ggtcgcggcg agcgacctga cggacggctc cgacctgagc ggacgggcga
     3661 cggtatcgct gtaccgtt
//
LOCUS       DAOPHV010000236         3677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_34497_length_3677_cov_4.250414, whole genome shotgun sequence.
ACCESSION   DAOPHV010000236 DAOPHV010000000
VERSION     DAOPHV010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34497_length_3677_cov_4.250414"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLQ29_07180"
     CDS             <1..301
                     /locus_tag="PLQ29_07180"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HPG86468.1"
                     /translation="NPFVVRAMAEVGLDISGNPTKSVFVLRETGRSYDVAVTVCSKEA
                     AERCPIFPGLAERRHWPFDDPSSFTGSDEAIMARVRSVRDEIEAAVLDFVESTTD"
     gene            complement(386..1258)
                     /locus_tag="PLQ29_07185"
     CDS             complement(386..1258)
                     /locus_tag="PLQ29_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPG86469.1"
                     /translation="MKKAILILLAAALVARAFARDVDAVVGVAWLEANLSKPGLVIVD
                     VRKIDEYKAGHVPGAVNLLGLYVTKDGLSNEVPEADDLSEIIAEAGIDGKSTVVVVES
                     GGARFAWATRVAWTLVYAGVPNVTILDGGYEEWVKAGKAVATGFESKDETDFEVTFVD
                     SYLATKDYLVKNLKKVQVVDTRVYDTYFGLSKQAFVDQYGHIPGAYALPAAWITVNGL
                     VRPKAELEELVKALKLDPKKEAVIHCDSGVLCTAWWWILTQQLGWTKVRSYDGSAQEI
                     SKDPSVKFVKAVWR"
     gene            complement(1289..2305)
                     /locus_tag="PLQ29_07190"
     CDS             complement(1289..2305)
                     /locus_tag="PLQ29_07190"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPG86470.1"
                     /translation="MGYVVSQDVLDSDPHFYLGCTDCHDGDSTKATQAEAHTGLVARP
                     SDDPVASCGQCHEETAKTYTLSLHYTAAGMKHGVSPRFDEKGKQLFDTKVFEQSCRSC
                     HASCGDCHVKSPVISGVNTGLLDGHRFVAKAEDKTCALCHGGRVWPEFTGDYGGTPDV
                     HYRKGMTCSDCHGPLQFHGDGNAYASRKDVAVRPLCSDCHDVTTMGKASTKETHATHA
                     DTVSCSACHTASAYRQCADCHVGAGATATPAIILGQNPRKEGQLTTLRLIPTVRDTFK
                     PAGVAMQSFDALPNYWDTVPHNVAKRTDRTRDCNTCHEEGLYYLEAERLPKGGSTKNL
                     ELVP"
     gene            complement(2390..2512)
                     /locus_tag="PLQ29_07195"
     CDS             complement(2390..2512)
                     /locus_tag="PLQ29_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86471.1"
                     /translation="MNKATSRRAMLMIAFLGAGALTVYATGSSCLSCHTNPATM"
     gene            2756..>3677
                     /locus_tag="PLQ29_07200"
     CDS             2756..>3677
                     /locus_tag="PLQ29_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HPG86472.1"
                     /translation="MKKFLDWITSMRLAIGLIAYLAATCVLATIVPQGLSDDAYREMY
                     SRIVAQLVVQTGFGSFFGSILFIIPAFLFFANLSACTVKRLVRELRKKKGRRHGPDVL
                     HLGLMALVIGSIWSFSGHQQGTVTLAPGDGVNLPDGAVMHLDDFRFDRYEDGRPKDWV
                     SVLSIEKDGRSVKEGVELRVNDPLRYGGLTFYQASYQEVAALALGGPGGEEYLLSQGE
                     DLVVGDSTYFFMAPDQDGSRAVMRVSSGADGRTVKASPGEAVGGLTVLGLRGRYAAGI
                     EAVSDPGFPLVLVAMALIAVGTTWTFAQKLK"
BASE COUNT          587 a         1161 c         1227 g          702 t
ORIGIN      
        1 caatccgttc gtcgttcgcg cgatggcgga ggtcggtctc gacatctcgg gtaacccgac
       61 gaagagcgtc ttcgttctcc gcgagacggg tcgttcctac gatgtcgccg tcacggtctg
      121 ctccaaggaa gcggccgagc gttgcccgat ctttcccggc ctcgccgagc gccgccactg
      181 gcccttcgac gacccctcgt cgttcacggg gagcgacgag gctatcatgg cgcgcgtcag
      241 gtcggtccgc gacgagatcg aggccgcggt cctcgacttc gtcgagtcga cgacggactg
      301 atacaaaagc cgcccccctg agggcgcgcc cccgcgaggg cgcgcacccg tgagggagcg
      361 gccgagccgt gcgtaacgct ctagcttagc gccagacggc cttgacgaac ttgaccgatg
      421 gatccttcga gatctcctgg gccgagccgt cgtaggagcg aaccttggtc caaccgagct
      481 gctgggtcag gatccaccac caggcggtgc aaaggacgcc agaatcgcag tggataacag
      541 cttccttctt cggatcgagc ttgagcgcct tgaccaactc ctcgagttcg gccttggggc
      601 gcacgagtcc gttcaccgtg atccaggcag caggaagcgc gtaggcgccg gggatatggc
      661 cgtactgatc gacgaaggcc tgtttcgaga ggccgaagta cgtgtcgtag acgcgcgtat
      721 cgacgacttg gacctttttc aggttcttga ccaggtagtc cttcgtcgcc aggtaggagt
      781 cgacgaacgt aacctcgaag tcggtctcgt ccttggactc gaagccggtg gctacggcct
      841 tgccggcctt gacccattcc tcgtaaccgc cgtccaggat cgtcacgttg ggcacgccgg
      901 cgtagacgag ggtccaggcc actctggtcg cccaggcgaa gcgggcgccg cccgactcga
      961 cgacgaccac ggtcgactta ccgtcgatgc ccgcctcggc gatgatctcg gacaggtcgt
     1021 cggcttcggg cacctcgttg gacaggccgt ccttggttac gtacaggccg agcaggttga
     1081 ccgctccggg cacgtggccg gctttgtact cgtcgatttt acgcacgtcg acgatgacga
     1141 ggccgggctt ggataggttg gcctcgagcc aggcgacgcc gacgacggcg tcgacgtctc
     1201 gggcgaaggc ccgggcgacg agcgccgcgg ccagcaggat caggatcgct ttcttcatcg
     1261 aaaactcctt catggtgctt catgcccatc agggcacgag ttccaggttc ttcgtcgagc
     1321 cgcccttggg cagtcgctcg gcctcgaggt agtacaggcc ttcctcgtgg caggtgttgc
     1381 agtcgcgggt gcggtcggtg cgcttggcta cattgtgggg caccgtgtcc cagtagttcg
     1441 gcagcgcgtc gaagctctgc atggctacgc cggccggctt gaacgtgtcg cgcacggtcg
     1501 gtatcagcct gagcgtggtc agctggcctt ctttcctggg gttctgcccg aggatgatag
     1561 ccggcgtggc ggtcgctccg gctccgacgt ggcaatcggc gcactggcgg taggcgctcg
     1621 cggtgtggca ggccgagcac gagaccgtgt cggcgtgcgt cgcgtgggtc tccttggtcg
     1681 aggccttgcc catcgtcgtc acatcgtggc aatccgagca cagcggcctg accgccacat
     1741 ccttacggct cgcgtacgcg ttgccgtcgc cgtgaaactg gagcgggccg tggcagtcgg
     1801 agcaggtcat gcccttgcgg tagtgcacgt cgggagtacc gccgtaatcg ccggtgaact
     1861 ccggccagac gcggcctccg tggcacagcg cgcaggtttt atcctcggcc ttggccacga
     1921 aacggtggcc gtcgaggagg cccgtgttca cgccggagat gacgggactc ttgacgtggc
     1981 agtcgccgca cgaggcgtgg caactacggc acgactgctc gaagaccttg gtgtcgaaca
     2041 gctgcttgcc cttctcgtcg aagcgcggcg atacgccgtg cttcataccg gcggcggtat
     2101 agtgaaggga cagcgtgtag gtcttggccg tctcttcgtg gcactggccg caactcgcca
     2161 cggggtcgtc ggacggcctg gccacgagtc ccgtatgggc ctccgcctgg gtcgccttgg
     2221 tcgagtcgcc gtcgtggcag tcggtacagc cgagatagaa atgcgggtcg ctatcgagaa
     2281 cgtcctggga tactacgtac cccatgtaat aatccgcccg aaccggggga ggagttcccg
     2341 ctcacccttc gccttcggcg gattcgcccg agggcggtat gacgagcttt tacatggtgg
     2401 ccgggttcgt gtggcaggag aggcagctcg agcccgtagc gtatacggtg agcgctcccg
     2461 cgccgaggaa cgcgatcatc agcatcgccc gccttgaggt cgccttattc actgtggaac
     2521 ctccttgcga gtcgttaagc gggccgacgg cctcggcggc tccttacgcc gcgctaccag
     2581 catactcgag tgccgatcgc ttgtaaatat ataaaagaat aactatatat taatattcta
     2641 gtaaagttga caaaagcgcc tccaaaccgt agtctcccat gccgggttcg aggcggcccg
     2701 ccggggtagt atccgtggct cagccgccat catctaggtt cggaggctgg cagacgtgaa
     2761 gaaattcttg gattggatca cctcgatgcg gctggccata ggcctgatcg cgtacttggc
     2821 cgcgacctgc gtgctggcca cgatagtgcc gcaggggctg tccgacgacg cgtaccggga
     2881 gatgtactcg aggatcgtcg ctcagctggt cgtgcagacc gggttcggct cgttcttcgg
     2941 gtcgatactg ttcatcatcc cggccttcct gttcttcgcc aacctgtcgg catgcacggt
     3001 caagcgcctg gtccgcgagc tgcgcaagaa gaaggggcgg cgccacggac cggacgtgct
     3061 gcacctcggg ctgatggccc tggtcatcgg cagcatctgg tcgttctccg ggcatcagca
     3121 gggcacggtg acgctcgccc ctggcgacgg cgtcaacctg ccggacggcg ccgtgatgca
     3181 tctcgacgat ttccgcttcg accgctacga ggacggccgg cccaaggact gggtctcggt
     3241 cttgtcgata gagaaggacg gcaggtcggt taaggagggc gtcgagctcc gggtgaacga
     3301 cccgctgcgc tacggcggcc tgaccttcta ccaggccagc taccaggagg tggcggcgct
     3361 ggctctcggc ggccccggcg gcgaggagta cctgctgtcc cagggcgagg atctcgtcgt
     3421 cggcgactct acgtatttct tcatggcgcc cgaccaggac ggctcgcgcg ccgtgatgcg
     3481 ggtctcgtcg ggagccgacg ggcgcaccgt caaggcctcg ccgggcgagg cggtcggcgg
     3541 cctaaccgtg ctgggcctgc gcggccgcta cgccgccggc atcgaggcgg tgtccgaccc
     3601 cggtttcccc ctggtcctcg tcgccatggc cctgatagcc gtcggcacga cctggacctt
     3661 cgctcagaag ctcaagg
//
LOCUS       DAOPHV010000237         3668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_34615_length_3668_cov_3.863272, whole genome shotgun sequence.
ACCESSION   DAOPHV010000237 DAOPHV010000000
VERSION     DAOPHV010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34615_length_3668_cov_3.863272"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PLQ29_07205"
     CDS             complement(<1..725)
                     /locus_tag="PLQ29_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HPG86473.1"
                     /translation="MKVAINGFGRIGRLVFQSIVDQGLLGKDKIDVVAVVDVATDAKY
                     FAYQLKYDSVHGRMNAKISSKKSDPSLEEDDVLVVNGHEVKCVMAEKELKNLPWAKLG
                     VEYVIESTGIFSNEKAYDHLEAGAKKVIISAPAKSKDESKKIKTFVVGVNENDYDASK
                     HHVISNASCTTNCLAPVVHVLLKEGIGIETGLMTTIHSYTATQKTVDGVSKKDWRGGR
                     AAAINIIPSTTGAAKAVGEVIPST"
     gene            1032..>3668
                     /locus_tag="PLQ29_07210"
     CDS             1032..>3668
                     /locus_tag="PLQ29_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HPG86474.1"
                     /translation="MASGKSVSKVLKILIVSAVVVAIALGVAFGVAIASVRNIMSVEN
                     FSTFDPPLPSRLLASDGRLITEFASSEKRELVSIKDMPQHLIDALVTREDQSFWTHPG
                     FTLKGYFRAFVGIVTGRNMGGGSTLTLQLAGTLYADRRDISFRRKFVELWWALQLERR
                     FSKQEILEMYMNRMIMGPGVYGVEAASKYFFGHSAKDVSIAESAILVIQLSSPTRYNP
                     LRNPNVARDRSREVLNQMIELGYVTKDAADESFALYWDNYDYTRVASGAFYARDDKAP
                     WFSEYVRRDLEKMLYGSIDLYKDGLTIHTTLDLDYQAVADKYMSRYIAQVNRDYASTS
                     SRRLKEAERIYAPMAELLGLAFNLEGMFFEETKVRGKAMEYYRKSVNPTVDAMALMFG
                     LEPLKLATNASYGAAKTEMEKNTVEGALITIDNATGHIKAIVGGSKFEQSNQLIRATQ
                     AQLMPGSCFKPLYYSAAIDSRRFTPGTLIYDAPVVFYNEDGTPYIPLNYKGEWQGQVL
                     TWNALAKSMNVPAVKVLDGIGFDAAINRAAALLDITDPEQVRTTFPRVYPLALGISAV
                     TPLRMARAFAVFANQGREVTPIAIRSIEDRDGRPLLDPEKDLRAEQKKKGSGIQVVSP
                     QNAYVMTDMLQRVVKVGTLAWPTSSGAKFTYRDADGKRYTIPAAGKTGTTQNWADAWT
                     VGFTPYMTTAIWFGFDRPGNSLGVSQSGAAIAGIAWADYMAEIHRGLPAKDFIRPQSG
                     LIDVTVCSVSGLLPTEYCDEGTLTLTYYEGTQPKSYCDLHEFKSEQAETTIRTLSSQQ
                     AVVGGGRVDSSLDFDPSSLDSLLRSLEADIERYRVEDGAGSATVPEPADPPGRFDPFD
                     PFAGASVPGTERSPSAESPTAED"
BASE COUNT          710 a         1180 c         1077 g          701 t
ORIGIN      
        1 gtgctcggaa tcacctctcc gactgccttg gcggcaccgg tcgtcgacgg gatgatattg
       61 atggcggcgg ctcgaccgcc gcgccagtcc ttcttggaca caccgtccac ggttttctgg
      121 gtggcggtgt agctatggat ggtcgtcatc aggccggtct cgatgccaat gccttcctta
      181 agcagtacat ggacgaccgg tgcgaggcag ttggtcgtgc aggacgcgtt cgagatgacg
      241 tgatgcttgg aggcgtcgta atcgttctcg ttgacgccga cgacgaaggt cttgatcttc
      301 ttggattcgt ccttgctctt ggccggagcg ctgataatga ccttcttggc tcccgcctcg
      361 aggtgatcgt acgccttctc gttcgagaag atgccggtgg actcgatgac gtactcgacg
      421 cccagcttgg cccagggcag gttcttgagc tccttctcgg ccatgacgca cttgacctcg
      481 tggccgttga ccacgagcac gtcgtcctcc tcgagcgacg ggtcgctttt cttggaggag
      541 atcttggcgt tcatgcggcc gtgaactgaa tcgtacttga gctggtaggc gaagtacttg
      601 gcgtccgtgg cgacgtccac tacggccacc acgtcgatct tgtccttgcc gagcagtccc
      661 tggtcgacga tagactggaa gaccagccgc ccgatccggc cgaaaccgtt gattgcaacc
      721 ttcattgagg cctcctatgc aaaagatgaa tcgataaaac agtatcttat tttctcgaat
      781 gatatcgata agtagaatcg ttcgtcaatg cgtggataaa tatacgcaat catgcgttcc
      841 ctatgcaatt atttatcttt aaaaaacgcg ttccggttcc gatatataga atgtcgggcg
      901 ccgtcgcggc ccccctccca attcgtcgcg gcggaggcag acagcctgtc agggcgggcc
      961 ggtcttcggg accggccttt tttatatcca gcttgaccta ccggcgagct ttcgcgtagg
     1021 ctttcggcga tatggcatcc gggaagtccg tcagcaaagt attgaagata ttgatcgtct
     1081 ccgccgtcgt cgtcgccata gcgctcggcg tcgcgttcgg ggtcgctata gcgtcggtac
     1141 gcaatatcat gtcggtcgag aatttctcca ccttcgaccc gcccctgcca tccaggctac
     1201 tcgctagcga cgggcggctc attaccgagt tcgcctcgag cgagaagcga gagctcgtct
     1261 cgatcaagga tatgccgcag cacctgatcg acgccctcgt gacccgcgag gatcagtcgt
     1321 tctggaccca ccccggcttt acgctcaagg gctacttcag ggccttcgta ggcatcgtga
     1381 ccggacggaa tatgggcggc ggctccacgc tgaccctgca gctcgccggc accctgtacg
     1441 ccgatcgtcg ggatataagc ttcaggcgca aattcgtcga gctgtggtgg gccctccagc
     1501 tcgagcggcg cttctcgaag caggaaatcc tcgagatgta catgaaccgc atgatcatgg
     1561 ggccgggagt ctacggcgtc gaggcggcca gcaagtactt cttcgggcat tccgccaagg
     1621 atgtcagcat cgccgagtcg gccatcctcg tgatccagct gtcgagccct acccgttaca
     1681 atccactgag gaaccccaac gtcgcgaggg atcgctcgcg cgaggtgctc aaccagatga
     1741 tagagctcgg ctacgtgacg aaggacgccg cagacgagtc cttcgcgctg tactgggaca
     1801 actacgacta tacccgcgtc gcctcgggcg cgttctacgc ccgcgacgat aaggccccct
     1861 ggttcagcga gtacgtccga cgcgacctcg agaagatgct gtacggctcg atcgacctct
     1921 acaaggacgg actaaccatc cacacgacgc tcgacctcga ctatcaggcg gtggccgaca
     1981 agtacatgag ccgctatata gcccaggtca accgcgacta cgcctcgacc agctcgcgcc
     2041 gcctcaagga agccgagcgg atctatgcgc cgatggccga gctgctcggc ctcgccttca
     2101 acctcgaggg gatgttcttc gaggagacca aggtccgagg caaggccatg gaatactacc
     2161 ggaagagcgt caaccccacc gtggacgcga tggcgctcat gttcggcctc gagccgctca
     2221 aactggcgac caacgccagc tacggagccg ccaagaccga gatggagaag aataccgtcg
     2281 agggcgcgct catcaccatc gacaacgcga ccgggcatat caaggcgata gtcggcggct
     2341 caaagttcga gcagtccaac cagctgatac gcgcgaccca ggcccagctg atgcccggct
     2401 cctgcttcaa gcctctctat tactccgccg cgatcgactc gaggcgcttc acgcccggca
     2461 ccttgatcta cgacgcgccg gtagtcttct ataacgagga cggtacgccg tacatcccgc
     2521 tcaactacaa gggcgagtgg cagggccagg tgcttacctg gaacgcgctg gccaagtcca
     2581 tgaacgtgcc ggccgtcaag gtgctcgacg gcatcggctt cgatgcggcc ataaaccgcg
     2641 cggccgccct gctcgatatt accgacccgg agcaggttcg taccaccttc ccgcgggtct
     2701 atccgttggc cctcggtatt agcgccgtaa cgccgctacg catggccagg gccttcgcag
     2761 tattcgccaa tcagggccgc gaggtcacgc cgattgccat acggtcgatc gaggacaggg
     2821 acggacggcc cctcctcgac cctgagaagg atctgcgcgc ggagcaaaag aagaagggct
     2881 ccggcataca ggtcgtctcc ccgcagaacg cctacgtgat gaccgacatg ctacagcgcg
     2941 tcgtgaaggt cggtaccctg gcctggccga cctcgtccgg agccaagttc acctatcgcg
     3001 acgccgacgg caagcgttat accatacccg cagcaggcaa gaccggcacg acccagaact
     3061 gggcggacgc ctggacggtg ggcttcacgc cgtacatgac gacggcgatc tggttcggct
     3121 tcgacaggcc gggcaactcg ctcggcgtca gccagtccgg cgccgccatc gcgggcatag
     3181 cctgggccga ctacatggcc gagatacacc gggggctgcc ggccaaggat tttatccgcc
     3241 cccagagcgg cctcatcgac gtcaccgtct gctccgtctc cggcctcttg ccgactgaat
     3301 attgcgacga gggcaccctg acgctcacct actacgaggg cactcagccc aagagctact
     3361 gcgacctgca cgagttcaaa tccgagcagg ccgagacgac catccggacg ctatcgtccc
     3421 agcaagccgt cgtgggcggc ggcagggtcg attcgagcct cgacttcgac ccgagttccc
     3481 tggattcgct gctacgctcc ctcgaggccg acatcgagcg ctacagggtc gaagacgggg
     3541 ccggttccgc gaccgttccc gaaccggccg atccgcctgg acgattcgat ccgttcgacc
     3601 ccttcgccgg ggcctccgta ccgggaaccg agcgcagccc ctcggccgag tcgccgaccg
     3661 ccgaggat
//
LOCUS       DAOPHV010000238         3644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_34943_length_3644_cov_3.808303, whole genome shotgun sequence.
ACCESSION   DAOPHV010000238 DAOPHV010000000
VERSION     DAOPHV010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34943_length_3644_cov_3.808303"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1998
                     /locus_tag="PLQ29_07215"
     CDS             <1..1998
                     /locus_tag="PLQ29_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HPG86475.1"
                     /translation="LFGVLDAMLQRAVHLAFGMALIFLLYPTSKKWSKDRLHPLDLGL
                     AVLGAITPMYIIANYGQLVLRAGTATTMDMIVGVVGILLVLEAARRIVGLPIVIIAGV
                     FIAYAFAGPWLPGQLAHRGVDVGDLVQHLYFTTEGVFGIPLGVSSTFIFLFILFGAYL
                     EKTGLGQYFIDLANSIAGRSAGGPAKVAVLSSGLMGTVSGSSVANVVGTGSFTIPMMK
                     RLGYKPEFAGAVEATASTGGQLMPPIMGAAAFLMSEMTGIPYVRIIGAAVIPALLYYF
                     GVWAGVHFEAKKLGLRGMTKDELPNLKEIFFGRGYLMIPLVGIVWLLVTGYTPMLAAF
                     YAIVLSIAAAVLGWWAPLPIGGMLVVFAIMKPIFKVGPYGADGALRYLTQMTPLGALA
                     VLGAILLGFAILKKKPNIGFKEIVGGLESGARGAIGVLAATACAGIIIGVVTKTGLGL
                     KLGSVLVSLANGNLILTLFFTMITSIILGMGVPTTANYIITSTIAAPAIIMLLRMKFP
                     ELPIDSISIVLPAHMFAFYFGIIADVTPPVALAAFAGAGIAKANPIKTGLAASKLAIA
                     AFLVPYIFVMNPMMLMIDVTFWSGLLMVATSMLGIIGVSSGIMGYLLCRSLWWERVLM
                     AAGGVLMIDPGAVTDIIGIGLIVVGVVSQVIRKRVDSGKTA"
     gene            2041..2721
                     /locus_tag="PLQ29_07220"
     CDS             2041..2721
                     /locus_tag="PLQ29_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HPG86476.1"
                     /translation="MSGIFDVIYEDGDVVIVNKLAAVPTLPDKSGDSSLKDFVAESRP
                     GRFVEPAHRLDRRTSGAVAFAKSKHSLAALSEAFRERRVTKTYWAVTERAPEPAAGTL
                     RHRLIHDKRRNQAKAVPANGDRGGDVAVLTYELVGRSDRYFLLAVRPLSGKTHQIRAQ
                     LSAAGFPVRGDLKYGSRRSTRNGLIMLHARELSFEMPDRRRVYVTADPPPDEPLWEAF
                     VEDPQNVN"
     gene            complement(2718..3425)
                     /locus_tag="PLQ29_07225"
     CDS             complement(2718..3425)
                     /locus_tag="PLQ29_07225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPG86477.1"
                     /translation="METEIRIDQAFFSDLERPASFYILRHGETVANAESRIQGRSEYP
                     LNENGRAQAADLARHLEGAGVRRLLYSPLSRARETAAIVAAALGLDGSELAAEPALVE
                     LDTGRFSGLTLTEIRERFPAEHEAFGYRSWEAVPDAERAEELYRRASLAWSRLKAEAK
                     ASGGNVAAVSHGGMIQWLVRATFGCRSWMPLLSTGNCGVFELFVDPVGGGRPAYLQWK
                     ELNRLPGGTAGRTPPVF"
BASE COUNT          592 a         1279 c         1134 g          639 t
ORIGIN      
        1 ctgttcggcg tacttgatgc gatgctccag cgcgcggtcc acctcgcgtt cggcatggcg
       61 ctgatcttcc tgctctaccc gaccagcaag aaatggtcca aggaccggct ccacccgctc
      121 gacctgggcc tcgcggtact aggcgccatc acgcctatgt acatcatagc caactatggg
      181 cagctcgtac tgcgcgccgg cacggcgacg acgatggaca tgatcgtcgg cgtcgtaggc
      241 atcctgttgg tactcgaggc ggccaggcgc atcgtcggcc tgcccatcgt catcatcgcg
      301 ggcgtcttca tagcctacgc cttcgccggc ccctggcttc ccggccagct ggcgcaccga
      361 ggcgtcgacg tcggagacct ggtgcagcac ctgtacttca ccaccgaggg cgtcttcggc
      421 ataccgctcg gcgtgtcgag caccttcatc ttcctgttca tactgttcgg cgcctacctc
      481 gagaagaccg gcctcggtca gtacttcatc gacctggcca actcgatagc cggccgctcc
      541 gccggcggcc ccgcgaaggt cgccgtcctg tcgagcggcc tgatgggtac ggtctcgggc
      601 agctcggtcg ccaacgtcgt cggcacgggc tcctttacta tccccatgat gaagcgcctg
      661 ggctacaagc ccgagtttgc cggagccgtc gaggccaccg cgtcgaccgg aggccagctg
      721 atgccgccta tcatgggcgc cgcggccttc ctgatgtcgg agatgaccgg tataccctac
      781 gtgcgcatca taggcgcggc cgtgataccg gccctgctct actacttcgg cgtctgggcc
      841 ggcgttcact tcgaggccaa gaagctcggc ttgcgcggca tgactaagga cgagctgccg
      901 aacctcaagg agatcttctt cgggcgcggc tacctgatga tcccgctcgt cggcatcgtc
      961 tggctgctcg tcaccggtta taccccgatg ctggcggcgt tctacgccat cgtgctgtcg
     1021 atagcggccg ccgtgctcgg ctggtgggcc ccgctgccga taggcggcat gctcgtcgtc
     1081 ttcgcgatca tgaaaccgat cttcaaggtc ggcccctacg gcgccgacgg cgcgctcagg
     1141 tacctgaccc agatgacccc gctcggcgcc ctcgcggtgc tcggcgccat cctactcggc
     1201 ttcgcgatcc tcaagaagaa gccgaacatc ggcttcaagg agatcgtggg cggcctcgag
     1261 tcgggcgcca ggggcgccat cggcgtactc gccgcgacgg cctgcgccgg tatcatcatc
     1321 ggcgtggtca ccaagaccgg cctcggcctc aagctcggct cggtgctcgt cagcctggcc
     1381 aacggcaacc tgatcctgac cctgttcttc acgatgatca ccagtatcat cctgggcatg
     1441 ggcgtgccga cgaccgcgaa ctacatcatc acctcgacga tagcggcccc ggccatcatc
     1501 atgctgctca ggatgaaatt ccccgagctg cccatcgact ccatctcgat cgtgctgccc
     1561 gcccacatgt tcgcgttcta cttcggcatc atcgccgacg tgacgcctcc ggtggcgctc
     1621 gcggccttcg ccggcgcggg catcgccaag gccaacccga tcaagaccgg cctcgccgcg
     1681 tccaagctgg ccatcgcggc cttcctcgtg ccgtatatct tcgtcatgaa cccgatgatg
     1741 ctgatgatag acgtgacctt ctggtccggc ctcctgatgg tcgcaacctc gatgctcggc
     1801 atcatcggcg tatcgtccgg catcatgggc tacctgctct gccgctcgct gtggtgggag
     1861 cgcgtcctga tggccgcagg cggcgtgctg atgatcgatc ccggtgccgt gaccgacatc
     1921 atcggcatcg gcctcatcgt agtgggcgtg gtctcccagg tgatacggaa gcgcgtcgac
     1981 tccggtaaaa ccgcgtaaga attctagcca aagaagcgcc ggatatggta ctatcgtgcc
     2041 atgtccggca tctttgacgt gatctacgaa gatggcgacg tcgtcatcgt caataagctc
     2101 gccgcggtgc cgaccctgcc cgataagagc ggcgactcgt ccctgaagga tttcgtcgcc
     2161 gagtcgaggc cgggccgctt cgtcgagccg gcccacaggc tcgaccgacg cacgagcggc
     2221 gcggtcgcct tcgccaagtc gaagcacagc ctggcggccc tatccgaggc cttccgcgaa
     2281 cgccgcgtga ccaagaccta ctgggccgtc accgagcgcg cccccgagcc ggccgccggc
     2341 acactacggc accggctcat ccacgacaag cgacgcaacc aggccaaggc cgtaccggcc
     2401 aacggcgacc gcggaggcga tgtagcggta ctgacctacg agctggtcgg ccggagcgac
     2461 cggtactttc tgctggcggt acggcccctg tcgggcaaga cgcaccagat acgggcccag
     2521 ctgtccgcgg caggcttccc ggtgcgcggc gacctgaagt acggctccag gcgatcgacg
     2581 cggaacggcc tgattatgct gcacgcccgc gagctgagtt tcgagatgcc tgaccgccgc
     2641 cgcgtatacg tcacggccga cccgcccccg gacgagcccc tctgggaggc cttcgtcgag
     2701 gacccgcaga acgtgaacta gaacaccggc ggcgtacggc ccgccgtacc gccgggtagc
     2761 cgattcagtt ccttccactg cagataggcg gggcggcctc cgcccaccgg gtcgacgaac
     2821 agctcgaaga cgccgcagtt gcccgtggac agaagcggca tccagctacg gcagccgaag
     2881 gttgcgcgga cgagccactg gatcatgccg ccgtgcgaca cggccgccac gttgccgccc
     2941 gaggccttag cctcggcctt gagcctggac caggcgagcg acgcgcgtcg gtagagctcc
     3001 tccgcgcgtt cggcgtccgg cacggcctcc caactgcggt acccgaaggc ctcgtgctcc
     3061 gccgggaaac gctcccgtat ctccgtcaag gtaagcccgg agaaacgccc cgtgtccagc
     3121 tcgacgaggg caggctcagc cgcgagttcc gagccgtcga ggccgagggc cgccgcgacg
     3181 atggccgccg tctcgcgggc gcgtgagagc ggcgagtaca gcaggcggcg cacgcccgct
     3241 ccctcgaggt gcctggctag gtccgcggcc tgggctcgcc cgttctcgtt cagagggtac
     3301 tccgaacggc cctggatgcg gctctcggcg ttggcgaccg tctcgccatg cctgaggata
     3361 tagaagctcg cgggccgctc gaggtcggaa aagaaggcct ggtcgatacg gatttcggtt
     3421 tccatcgccg gcgactatag caccgggacg acgggcggct caagcgcgct ttaccacccg
     3481 ccgcggcgag gcgtatactg ccccgatgca ccgacgttcg atcaccaacg acgccatcaa
     3541 aggcggcctc gtcctagcca tgatagccta ccacgcctgc tacgtcgggg cgatgttcga
     3601 cctggcccgg ctcgagctat tctccggctt ctggtggatg ttcc
//
LOCUS       DAOPHV010000239         3592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_35684_length_3592_cov_3.042409, whole genome shotgun sequence.
ACCESSION   DAOPHV010000239 DAOPHV010000000
VERSION     DAOPHV010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35684_length_3592_cov_3.042409"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..121
                     /locus_tag="PLQ29_07230"
     CDS             14..121
                     /locus_tag="PLQ29_07230"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HPG86478.1"
                     /translation="MGQSMGISAETVRQIEKRAIRKLREHTEYADFIGA"
     gene            184..1179
                     /locus_tag="PLQ29_07235"
     CDS             184..1179
                     /locus_tag="PLQ29_07235"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HPG86479.1"
                     /translation="MPTKRPRKSPRDRGASILLYTSATLVLMSFALAIALSSAGSRPD
                     RDARLPKADERQETAAPPAPSEAVPKGDGGAAGPAMTDTGPTVAPQRPQPVERPAPRS
                     RGVLVFVIDDAGHNRWQLEPFLKLPGPITIAVLPGLPHTANAASMVKAAGKELILHQP
                     MEALGGLDPGPGAIRADMDDGTIRDIIRGNLAQVPGARGVNNHMGSKATSDVRVMSTV
                     LDETSANGLYFLDSLTTGDSVVHSVASLMRQSAWERSVFLDNTPDKASILNYIAEGTK
                     IAEKRGYAVMIGHVWSSELAQTLADLYPQLLEQGFSLSTISRIMMDDDDDDDTGY"
     gene            1160..2167
                     /gene="tsaD"
                     /locus_tag="PLQ29_07240"
     CDS             1160..2167
                     /gene="tsaD"
                     /locus_tag="PLQ29_07240"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPG86480.1"
                     /translation="MTILGIESSCDECAAAIVADGRRVASNVVATQIARHAAFDGVVP
                     EIASRLHTEWIQDVVSKAIADAGIAEGAIDGVAVTARPGLAGSLLVGVSFAKAFAWSR
                     GLPFIGVNHVLAHLYAPLLDDEVEYPFIGLIVSGGHTLICRADGFDDVTVLGTTIDDA
                     IGEAFDKVAKHYGLGYPGGSAIDRLARAGDAAACDFPRPNLYKGEHRYDVSYSGLKTA
                     AIRQLDQFWNPAYERNDANLAAAFERAAVDMLVSRLLRALDDTGIRTVVAGGGVAANT
                     YLRSRLAGRAGLRVLFPPLSLCGDNAAMVAGLGYRMLERGDRDGLQLNASPRVAAFKR
                     S"
     gene            complement(2180..2599)
                     /locus_tag="PLQ29_07245"
     CDS             complement(2180..2599)
                     /locus_tag="PLQ29_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86481.1"
                     /translation="MTKPRLVIVLSAGAVLAVAAAVIVGVSLYSRSEAARESLVVRYF
                     RALSSGDEALVKDLVAPEFSSDLGLGNLVRGSYELFDFGEREPGTLRFLIIVQGEAGG
                     SGAERRAMLCELRYRRYGLTNRIGSIRLVESGVGLKE"
     gene            complement(2609..>3592)
                     /locus_tag="PLQ29_07250"
     CDS             complement(2609..>3592)
                     /locus_tag="PLQ29_07250"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPG86482.1"
                     /translation="EWGYGLSSVRFICGTQEPHKRLERACSEFLGTEDAILFSSCFDA
                     NGAVFEPLLDADCAIVADSLNHASIIDGVRLSKAERLIYQHGDFDDLEAKLKAAAGKR
                     LRLVATDGVFSMDGDIADLERICDLAERYDALVMVDDSHATGFIGKRGRGTHEYRGVM
                     DRVDIITTTFGKALGGASGGCVAGKAEIVEYLRQKARPYLFSNTLAPAIVGGTLKALE
                     LLTESTELRDRLESNTKRFRDGLARAGLDVRPGVHPICPVMLYDERLAHEMADRLLDE
                     GIYVIGFSFPVVPRGKARIRVQVSAAHSVEQIDAAVAAFAKVGAAMGAVKK"
BASE COUNT          636 a         1203 c         1179 g          574 t
ORIGIN      
        1 gacgctcaag ggcatcggcc agagcatggg tatatcggct gagaccgtgc ggcagatcga
       61 gaaacgggcc atccgcaagc tgcgcgagca taccgaatac gccgacttca taggagcctg
      121 acaaggctcc ctacggcggc ccttgcgaaa gactcggccc gcgtcgtata gtccgactat
      181 acgatgccta cgaaacgtcc gaggaagtcg ccgcgcgatc ggggcgcctc gatcttgctc
      241 tatacgagcg cgactctcgt gctcatgtcg ttcgcgctgg ccatagcgtt gtcaagcgcg
      301 ggctcccggc cagatcgcga tgcgcggcta ccgaaggctg acgagaggca ggaaacggcg
      361 gcgccgcccg cgccgtccga ggccgtcccg aagggagatg gcggagcggc cggaccggcc
      421 atgacggata cgggccccac cgtcgcgccc caacggccgc agccggtcga gcggcccgcc
      481 ccgcgatcca gaggcgtcct ggtcttcgtt atcgacgacg ccgggcacaa ccgatggcag
      541 ctcgagccgt tcctgaagct acccggaccg ataacgatag ccgtgctacc tggacttccc
      601 catacggcga acgccgcgtc gatggtcaag gcggcgggca aggagctcat actgcaccag
      661 cccatggaag cgctgggcgg cctagacccg gggccgggcg cgatacgggc ggacatggac
      721 gacgggacga tacgcgacat cattcgcggc aacctggccc aggtgccggg ggccaggggc
      781 gtgaacaacc atatgggctc caaggcgacc tccgatgtgc gggtgatgtc caccgtgctc
      841 gacgagacct ccgccaacgg gctctatttc ctggactctt taaccacagg cgattccgtg
      901 gtacactccg tggcttcgct gatgagacaa tccgcatggg agcgtagcgt gtttctagat
      961 aatacccccg acaaggcctc gatcctcaac tacatcgccg agggcaccaa gatagccgag
     1021 aagcgcggct acgccgtcat gataggccat gtctggtcat cggaactggc ccagaccctg
     1081 gctgacctgt acccgcagct cctggagcag ggtttcagcc tgtcgaccat atcgaggatc
     1141 atgatggacg acgacgacga tgacgatact gggtattgag tcgtcctgcg acgagtgcgc
     1201 ggcggccatc gtcgcggacg gccgccgcgt cgcctcaaac gtcgtggcca cgcagatcgc
     1261 caggcacgcc gccttcgacg gcgtcgtacc ggagatcgcc tcgcgcctac ataccgaatg
     1321 gatccaggac gtcgtatcga aagccatcgc cgacgctggg atagccgaag gcgccatcga
     1381 cggcgtggcc gtaacggcca ggcccggcct cgccggctcc ctgctcgtcg gtgtatcctt
     1441 cgccaaggcg ttcgcctggt cgcgaggtct gccttttatc ggcgtgaacc atgtgctggc
     1501 ccacctctac gcgccgctcc tcgatgacga ggtcgagtat ccattcatcg gcctgatcgt
     1561 ctccggcggc cacacgctga tatgccgggc cgacggtttc gacgacgtaa ccgtgctggg
     1621 cacgaccatc gacgacgcca tcggagaggc cttcgataag gtggccaagc actacggcct
     1681 cggctacccg ggcggtagcg ccatcgaccg actggcgcgc gcgggcgatg ccgcggcctg
     1741 cgacttccca aggccgaacc tgtacaaggg cgagcaccgc tacgatgtat cgtactctgg
     1801 actcaagacg gccgccatac gccagctcga tcagttctgg aaccccgcct acgagcgcaa
     1861 cgacgccaac ctggccgcgg ccttcgagag ggcggccgtc gacatgctcg tctcgaggct
     1921 actgcgagcg ctggacgaca cgggcatacg cacggtggtc gccggcggcg gcgtcgcggc
     1981 gaacacctac ctgcgatcgc gcctggccgg cagggctggg ctacgcgtac tcttcccgcc
     2041 actgtcgctc tgcggcgaca acgccgccat ggtcgccggc ctcggctacc gcatgctgga
     2101 gcgcggcgac agggacggcc tccagctcaa cgcctcgccg cgcgtcgccg cgttcaagcg
     2161 atcctagcga ggccccgccc tactccttga gcccgacgcc agactctacg agcctgatcg
     2221 agccgatccg attggtcagg ccgtaacggc gatagcgtag ctcgcagagc atggcccgcc
     2281 gctcggcccc cgagccgcca gcctcgcctt gcacgatgat caggaaacgc agggttccag
     2341 gctccctctc gccgaagtcg aacagctcgt agcttcctcg aaccaggttc ccgaggccta
     2401 ggtcggatga gaattccggc gctacgagat ccttgaccaa cgcttcatcg cccgaggaca
     2461 gggccctgaa gtagcggacg acgagcgatt ccctagccgc ttcgctacgc gagtacaggc
     2521 tgacgcccac gatgaccgcg gccgcgacgg ccaggacggc ccccgcggat aggacgatga
     2581 cgagcctcgg cttggtcatc gaactaccct acttcttgac ggcgcccatc gcggcaccga
     2641 ccttggcgaa ggccgccacc gcagcgtcga tctgctcgac cgagtgggcc gcggatacct
     2701 ggacacggat cctggccttg cccctgggca cgaccgggaa gctgaagcct atcacgtaga
     2761 tgccctcgtc gagcaggcgg tcggccatct cgtgggcgag gcgctcgtcg taaagcataa
     2821 ccgggcagat ggggtgcacg cccggacgta cgtcgaggcc ggccctggcc aggccgtccc
     2881 tgaagcgctt ggtgttcgac tcgagcctat cgcgcagctc ggtcgactcc gtcagtagct
     2941 cgagagcctt gagagtgccg ccgactatgg caggggcgag cgtgttggag aacaggtagg
     3001 gtctggcctt ctgcctgaga tactcgacga tctcggcctt gccggcgacg cagccgccgg
     3061 aagcgccgcc gagggccttg ccgaaggtcg tcgtgatgat gtcgacgcga tccatcacgc
     3121 cgcggtactc gtgggtgccg cggccgcgct tgccgatgaa gccggtcgcg tggctgtcgt
     3181 cgaccatcac gagggcgtca tagcgctcgg ccaggtcgca gatgcgctcg aggtcggcga
     3241 tgtcgccgtc catcgagaag acgccgtcgg tcgcgaccag tcgcagacgc ttgccggcgg
     3301 cggccttgag cttggcctcg aggtcgtcga agtcgccgtg ctggtagatc aggcgctcgg
     3361 ccttggacag gcgcacgccg tcgatgatcg acgcgtggtt gaggctgtcg gcgacgatgg
     3421 cgcagtcggc gtcgagcagg ggttcgaaca cggcgccgtt cgcgtcgaag caggacgaga
     3481 acaggatggc gtcctcggtg ccgaggaatt cggagcaggc gcgctcgagc cgcttgtgtg
     3541 gttcctgcgt gccgcagatg aagcggacgc tcgacagccc gtagccccac tc
//
LOCUS       DAOPHV010000240         3588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_35734_length_3588_cov_4.521087, whole genome shotgun sequence.
ACCESSION   DAOPHV010000240 DAOPHV010000000
VERSION     DAOPHV010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35734_length_3588_cov_4.521087"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLQ29_07255"
     CDS             <1..560
                     /locus_tag="PLQ29_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86483.1"
                     /translation="SKAAYAEDGSASSRVEYAYDADGNPIRETTLDGKGAVQSVSEWA
                     WEKGLRTSWRVSSGDGTVLARTDYYYDDGSLASARLYDGSGGPRGRVEYGYDADGALM
                     STRYYSADGKSDGRIEYRRDGNRLVEESVYRADGRLERKLTYGYGLDGELLRKTLHDG
                     SGKAREHVDYEYVFRIATETVVYYE"
     gene            587..1759
                     /locus_tag="PLQ29_07260"
     CDS             587..1759
                     /locus_tag="PLQ29_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86484.1"
                     /translation="MRIIRSAALAAALAIAISPAFSGEAARVYGRILAEAGSVQEKYA
                     AATAASSVDDPDLAPYLADALDWTLGARSTVRSGPERETYEKLTRVLLKRLGEARYAN
                     AAASAMRAVGDSTDPLTRAEALIALGSMRADEYAERIALLLRDLNNEPTADKQGGEQV
                     AYGCVIALERMRSPVGFAPLFFASEGWYSKRVRDQAERSLALVLADPSESVSALLRTE
                     TPPRMLRALALELRSQAPAAGKTAVAALALSRGIAYAPRNRVEQGQLSELRVVAMNAL
                     ASAGPGNGSAVPDMAEAYRIGPTDERLVAMKALGAEKSPASAAALRDIILDLNSAQKA
                     GLVDETRNALMRAALQNAAFNAGKELAPAVQAVLINGGWSSGVLSLASIAQKALQQ"
     gene            1783..2904
                     /locus_tag="PLQ29_07265"
     CDS             1783..2904
                     /locus_tag="PLQ29_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPG86485.1"
                     /translation="MTTYERRRHALAAKIADQNVAMAMFEDTEGRRDPSIRYFCGHPG
                     DALLFIAADGRSLLVPWDVNMAASMASVDAVAAYTDFSRSPFAAMAGALERLGVPGGS
                     VVDLPSVTPYPMYVDYVEACPDHDFRCSDDGSAKLAAEMRAVKDADEIATYRTVSAIT
                     DEAMDEIERGVRSGALATEADVALFIERFLRLKGCEATGFDTLAAGPSRSFGIHAFPA
                     YTAAAFATEGMSILDFGLSYKGYTSDVTMTFVRGSTAGRRGEMIALVQKAHDDAIAIC
                     GPGVRSRDVALLVDRVFEEKGYKMPHALGHGVGLEAHEAPAVRSREDNEWLLAPGHVI
                     TIEPGLYDPEAGGVRLEDDVLITESGCEVLTHARIVLLG"
     gene            complement(2957..>3588)
                     /locus_tag="PLQ29_07270"
     CDS             complement(2957..>3588)
                     /locus_tag="PLQ29_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPG86486.1"
                     /translation="ATQGRAEVEGEDPSASALDRERAFLGVVDGIVGSYPRLRVALEH
                     LSCAESVRAVLAWPERVGATITAHHLSYTIDDLLGGGLRPELFCKPVLKSAADRAALL
                     EAAVSGSPRFFFGSDSAPHPPEAKAAGAAGCYTAPVALSLLAAAFEEAGALDRLEAFV
                     SVSGARFYGLGLNPGVVRLSREGWTVPAAVDGAVTPAAGRTLAWTADRI"
BASE COUNT          585 a         1246 c         1240 g          517 t
ORIGIN      
        1 cgtccaaggc cgcctatgcc gaggacggct ccgcgtcgtc ccgcgtcgag tatgcctatg
       61 acgccgacgg caatcccatc agggagacga cgctcgacgg taagggcgcc gttcagtccg
      121 tatcggaatg ggcctgggag aagggcctcc ggacatcgtg gcgcgtctcg tccggcgacg
      181 gaaccgtcct ggccaggact gactattact acgacgacgg gagcctcgcg tcggccaggc
      241 tgtacgatgg ctcgggcggc ccgcgcggcc gggtcgagta cgggtacgac gccgacggcg
      301 cgctgatgtc gaccaggtat tatagcgccg acgggaaatc cgacgggcgc atcgagtacc
      361 gtcgcgacgg caaccggctg gtggaagaga gcgtgtaccg cgccgatggc aggctcgagc
      421 gcaagctgac ctacggctat ggcctcgacg gagagctact acgtaagacg cttcacgacg
      481 gctccggcaa agccagggaa cacgtcgact acgagtacgt gttcaggatc gcgacggaga
      541 ccgtcgtcta ctacgaataa tgggcgaagc ccgagtgagg aacaccatgc gaatcatcag
      601 gagcgcggcg ttagccgccg cgctggccat agccatatcg cccgcgttct ccggcgaggc
      661 cgccagggtc tacggccgca tcctggccga ggccggctcg gtgcaggaga agtacgccgc
      721 cgccacggca gcgtcgtcgg tcgacgaccc ggacctggcc ccatacctgg ccgacgcgct
      781 cgactggacc ctcggcgccc gtagcacggt ccggtcgggt cccgaacgcg agacctatga
      841 gaagctgacg agggtcctgc tcaagcggct cggcgaggct cgctacgcca acgcggcggc
      901 gtcggcgatg cgtgccgttg gagactcgac cgacccgctg accagggccg aagccctgat
      961 agcgctcggc tcgatgcgag ccgacgagta cgccgagagg atcgcgctcc tcctgcgcga
     1021 cctgaacaac gagccgaccg ccgacaagca gggcggcgag caggtggcct acggctgcgt
     1081 catcgcgctc gagcgtatgc gctcgccggt aggcttcgcc ccactattct tcgcgagcga
     1141 gggatggtac tcgaagcggg tcagggacca agccgagcga agcctggccc tcgtgctcgc
     1201 cgacccgagc gagtccgtct ccgcgctgct ccgtaccgag acgccgccgc gcatgctacg
     1261 cgccctcgcg ctcgagttgc gctcccaggc tccggccgcc ggaaaaacgg ccgtcgcagc
     1321 gctcgccctg agccgcggca tcgcctatgc gcctcgcaac cgcgtcgagc agggccagct
     1381 gtcggagcta cgagtcgtcg ccatgaacgc gctggcctcc gcggggccag ggaacggctc
     1441 agccgtgccg gatatggcag aggcctaccg tatcggcccg accgacgagc gcctcgtcgc
     1501 catgaaggca ctcggcgcgg agaaatcgcc cgcctcggcc gcggcattac gtgatattat
     1561 cctggacctg aactcggccc agaaggccgg gctcgtcgac gagaccagga acgctctgat
     1621 gcgggccgcg ctgcagaacg ccgcctttaa cgcgggcaag gagctggcgc ccgctgtcca
     1681 ggccgtgctg atcaacggcg gctggtcgag cggcgtgctc agcctcgcct cgatcgccca
     1741 gaaggcgctc cagcagtaac tacgagcttg aggaggtata ccatgactac ctatgagagg
     1801 cgtcgccatg ccttggccgc aaagatcgcc gaccagaacg tcgcgatggc catgttcgag
     1861 gacacggagg gccggcgcga tccttccatc cgctatttct gcgggcatcc gggtgacgcc
     1921 ctcctgttca tagcggccga cggccgttcc ctcctcgtgc catgggacgt gaacatggcc
     1981 gcctcgatgg ctagcgtcga cgcggtggcg gcgtatacgg atttctcccg tagccccttc
     2041 gcggcgatgg ccggcgccct ggagcgcctc ggcgtccccg ggggctcggt cgtcgacctg
     2101 ccatccgtca cgccctaccc gatgtacgtg gattacgtcg aggcctgccc cgaccacgac
     2161 ttccgatgct ccgatgacgg gtcggccaag ctggccgccg agatgcgcgc cgtcaaggac
     2221 gccgacgaga tcgctaccta ccgtaccgtc tccgccatca ccgacgaggc gatggatgag
     2281 atcgagaggg gcgtccgttc cggcgctctg gccaccgagg ccgacgtggc cctgttcatc
     2341 gagcgcttcc tccggctgaa gggctgcgag gccaccggct tcgataccct cgccgccggg
     2401 ccgtcgcgca gcttcggcat ccacgccttc cccgcgtata ccgcggcggc cttcgccacc
     2461 gagggcatgt ccatcctcga cttcggcctg tcgtacaagg gctatacctc ggacgtgacg
     2521 atgaccttcg tccggggctc gaccgccggt aggcgcggtg aaatgatagc cctcgtccag
     2581 aaggcccacg acgacgccat agcgatctgc gggccaggcg tcaggagccg cgacgtggcc
     2641 ctgctcgtcg accgcgtgtt cgaggagaag ggctacaaga tgccgcatgc gctcggccac
     2701 ggcgtggggc tcgaggcgca cgaggctccg gccgtacgta gccgggagga taacgagtgg
     2761 ttactcgcgc ccggccacgt gatcacgatc gagccgggcc tctacgaccc cgaggcgggc
     2821 ggagtcaggc tcgaggacga cgtgctgatc accgagtcgg gctgcgaggt tctcacgcac
     2881 gcgaggatag tcctgctcgg ctgacggttc gacgataggg tccgtatcgg ccgcccggcc
     2941 tggccgggcg gctttttcat atccggtcgg cggtccaggc gagcgtccgg ccggctgccg
     3001 gcgtcacggc gccgtcgacg gcggccggca ccgtccagcc ctcccggctg agccggacga
     3061 cgcctgggtt cagtccgagc ccgtagaagc gggctcccga tacggagacg aaggcctcca
     3121 gccggtcgag ggcgccggcc tcctcgaacg cggcggccag cagcgacagc gccaccgggg
     3181 ccgtatagca gccggccgcg ccggcggcct tggcctcggg cgggtggggg gcgctgtccg
     3241 acccgaagaa gaagcgcggc gagccggata cggccgcctc gagcagcgcg gctcggtcgg
     3301 ccgccgactt gagcacgggc ttgcagaaga gctccggccg caggccgccg ccgagcaggt
     3361 cgtcgatcgt atacgatagg tggtgggcgg tgatcgtggc cccgacccgc tcgggccagg
     3421 ccagcacggc ccggaccgac tcggcgcagg acaggtgctc gagcgcgacg cgcagccggg
     3481 gatacgagcc gacgatgccg tcgacgacgc cgaggaaggc gcgctcccga tcgagcgccg
     3541 aggccgacgg gtcctcccct tccacctccg cccgcccctg cgtggccc
//
LOCUS       DAOPHV010000241         3583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_35806_length_3583_cov_4.337302, whole genome shotgun sequence.
ACCESSION   DAOPHV010000241 DAOPHV010000000
VERSION     DAOPHV010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35806_length_3583_cov_4.337302"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1799)
                     /locus_tag="PLQ29_07275"
     CDS             complement(<1..1799)
                     /locus_tag="PLQ29_07275"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPG86487.1"
                     /translation="MFLREIGAPVSDLKGAGPSVCKRLARLGVFTGADLLLLAPRDYE
                     DRSAPRLLSEFGSGPVNCRVAVVAHDWFGFGRMRTLKVWVEDERGTRAALVCFNRPFL
                     ERSLPVGSSLKLYGRFDYRYSELQSSAFDAEPVDEVADGLPDGVRPVYPLTEGLGQST
                     MRKLVTGALARWGAVDDELPPSIRDRRGLLRKAEALRALHAPSTPAETVEAKRALAYE
                     ELFYLQVMIARRAASRRSDERPREPPPAGLALALEERLPFELTADQKAAVADIVADLS
                     GPYPMARLLQGDVGSGKTLVAFFAALYAIASGGQVAMMAPTELLARQHADTAARLLEP
                     LGVRLAFLSGNVADTARRPLLAALSRGEVDLVIGTHALFSGDVEYRNLSLAVVDEQQR
                     FGVLQRLALGAKGRKPDMLMMTATPIPRTLALTAFGDLAISTIRTMPKGRLPVVTHLA
                     KDGNEAKVYEFVRRELAAGRRAYFVYPLVDRSDRLALKDAEAMFERLSRDVYPEYQGG
                     LIHARLPEEKKRQTMADFAAGRLSFIVATSVVEVGVDVPEATCMVVEHAERFGLAALH
                     QLRGRVGRSDLQSYCFLVWSDGRAEDGARLSEDG"
     gene            complement(1807..2880)
                     /locus_tag="PLQ29_07280"
     CDS             complement(1807..2880)
                     /locus_tag="PLQ29_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86488.1"
                     /translation="MKRLSRFAAVVATAVAAGMAAASCASIQRDVYYDSPAGEDPAEL
                     AELESSLVRSRAVPGAGNLAAARARLAELASAPSSDSARSARILALSAEAAIQSGDRN
                     AAARLLAASKAAYRGDEVAAVVESRLAPTQAERLAVLDRSAAVADGGYRVGAERGSAL
                     LALGRIREAIVAFDAALPRLGEEYALLFGDERERAWSLRDAESAPSADASAYLTRVPI
                     TLLGMAAVAQSETTAMDRVTGGAPWAAGVLFERLKAAGWYIDSGADAKSPATRKDAAM
                     FLWTMMCRGDRAMAARYTARYASRGRSPVPDVPLGSPYFDAALGVVEEGVMALVDGSA
                     FDPDGPASGLDFYGWLLAAAAWR"
     gene            complement(2894..>3583)
                     /locus_tag="PLQ29_07285"
     CDS             complement(2894..>3583)
                     /locus_tag="PLQ29_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86489.1"
                     /translation="ALMLVSADGSTRFSSAFDSRQRSAPAVSGYALAALPRSFDSELY
                     LMDLEGMALPGWPVPLPGIASAAPAFAGADTAADGRALALTEAGQLLAYAIDAAASPG
                     FPVPLEGSFDAAPVWAPGWRSVYALSVEGTLYRVGLDGSTLGSVRVGRQSARGAAVTA
                     YDADGDGREELYVSGGGDALYAFSGDLSLLPGYPVSGAGAPRFIDIDGDGTDDMVVRG
                     ADDTLRAYSGR"
BASE COUNT          558 a         1269 c         1292 g          464 t
ORIGIN      
        1 ccgtcctcgg acaacctggc cccgtcctcc gcccggccgt cggaccagac gaggaaacag
       61 tacgactgca ggtcgctccg ccctaccctg ccgcgtagct ggtgcagtgc cgcgagcccg
      121 aagcgctcgg cgtgctcgac gaccatgcag gtagcctcgg ggacgtcgac gccgacctcg
      181 acgacgctcg tggcgacgat gaacgatagc cggccggcgg cgaagtcggc catcgtctgg
      241 cgcttcttct cctcgggtag cctggcgtgg atcaggccgc cttggtactc ggggtagacg
      301 tcgcgagaca ggcgctcgaa catggcctcg gcgtccttca gggccagccg gtcggaacgg
      361 tcgaccagcg gatagacgaa gtaggcgcgc ctaccggcag ccagctcgcg ccgtacgaat
      421 tcgtagacct tggcctcgtt gccgtctttg gccaggtgcg tgacgacggg cagtcggccc
      481 ttcggcatcg tgcgtatcgt cgatatcgcg aggtctccga aggcggtcag cgcgagggtc
      541 ctcgggatgg gcgtcgcggt catcatgagc atgtccggct tgcgtccctt ggcgccgagc
      601 gccagccgct ggagcacgcc gaaacgctgc tgctcatcga cgacggccag gctgaggttc
      661 cggtactcga cgtcgcccga gaacagggcg tgcgtgccga tgacgaggtc cacctcgcct
      721 cgggacaggg cggccaggag cggtcgcctc gcggtgtcgg cgacgttgcc cgacaggaag
      781 gcgagccgta cgccgagcgg ctcgagcagc ctggcggcgg tgtcggcgtg ctgcctggcc
      841 aggagttcgg tcggggccat catcgcgacc tggccgcccg aggctatggc gtagagcgcc
      901 gcgaagaacg cgacgagggt cttgcccgaa cccacgtcgc cctggaggag cctggccatc
      961 ggataggggc ccgatagatc ggcgacgatg tcggcgacgg cggccttctg gtcggccgtc
     1021 agctcgaacg gtaagcgctc ctcgagggcg agcgccaggc cggccggagg cggctcacga
     1081 ggccgctcgt cggagcgcct ggacgcggct cgcctggcta tcatcacctg gagatagaac
     1141 agctcctcgt aggcgagggc gcgcttggcc tcgaccgtct cggccggcgt cgaaggggcg
     1201 tgcagggcgc gcagggcctc ggccttgcgt aagaggccgc gccgatcgcg tatcgaagga
     1261 ggtagctcgt cgtccaccgc tccccagcgc gctagcgctc cggttacgag cttgcgcatc
     1321 gtcgactggc cgaggccctc ggtcagcggg tagacgggcc tgacgccgtc cggaagcccg
     1381 tcggccacct cgtctaccgg ctcggcgtcg aaggccgacg actgcagctc ggagtagcgg
     1441 tagtcgaagc ggccgtacag cttcaaggaa gagccgaccg gcaggctgcg ctcgaggaag
     1501 ggccggttga agcagacgag ggcggccctc gtcccgcgct cgtcctcgac ccagaccttg
     1561 agggtacgca tgcggccgaa gccgaaccag tcgtgggcga ccacggcgac gcggcagttg
     1621 acggggcccg acccgaactc cgacaggagc ctcggcgccg accggtcctc gtagtctcgc
     1681 ggcgcgagga gaagaaggtc cgcgccggtg aagacgccaa gcctggccag gcgcttacag
     1741 accgacggcc cggcaccctt gaggtcggat accggcgccc ctatttcacg gaggaacaac
     1801 gccgccctag cgccaggccg cggccgcgag cagccagccg tagaaatcga ggcccgaggc
     1861 cgggccgtcc gggtcgaagg cgctaccgtc gaccaaggcc atcacgccct cctccaccac
     1921 gccgagggcc gcgtcgaagt acggcgagcc gaggggcacg tccggcacgg ggctacggcc
     1981 ccgcgaggcg tagcgggccg tatagcgcgc cgccatcgcc cggtcgcccc ggcacatcat
     2041 cgtccagagg aacatcgccg cgtccttcct cgtggccggg gacttggcgt ctgcgcccga
     2101 gtcgatatac cagccggccg ccttgagccg ctcgaacagc acaccagcgg cccatggcgc
     2161 cccgccggtg acgcggtcca tcgcggtggt ctccgactgg gcgaccgccg ccatgccgag
     2221 cagggtgatg gggacgcgcg tcaggtacgc cgaggcgtcg gccgacggcg ccgactcggc
     2281 gtcgcgcagc gaccaggccc gctcgcgctc gtctccgaac aggagggcgt actcctcgcc
     2341 gagcctgggc agggccgcgt cgaaggcaac gatggcctcg cggatgcgcc cgagcgccag
     2401 gagggccgag cccctctcgg ccccgacgcg atagccgcca tcggctacgg ccgccgagcg
     2461 atcgaggacc gcgagccgct cagcctgggt cggagccagg cgggactcga cgacggctgc
     2521 gacctcgtcg ccccggtacg ccgccttgga ggcggccagc agcctggcgg ccgcgttccg
     2581 gtccccggac tggatggcgg cctcggccga cagcgcgagt atcctcgcgc tcctggccga
     2641 gtcgctcgac ggcgcggacg cgagctccgc gaggcgggct cgggccgcgg ccaggttgcc
     2701 ggccccgggc accgccctgg accgcacgag gctggactcg agttcggcga gctcggcggg
     2761 atcctccccg gccggcgagt cgtagtacac gtcgcgctgg atcgaggcgc aggaagcggc
     2821 ggccatgccg gccgcgacgg ccgtcgccac tacagccgcg aacctggaca atctcttcat
     2881 ctcgtccgtc ctttcagcga ccggagtagg cgcgcagggt atcgtcggcg ccgcgcacga
     2941 ccatatcgtc cgttccgtcg ccgtctatgt cgatgaaacg gggagcgcca gccccggaga
     3001 cggggtagcc gggcaatagc gataagtcac ccgagaacgc gtacagcgcg tcgccgccgc
     3061 ccgatacgta cagctcctcg cgaccatcgc cgtcggcgtc gtaggccgta acggcggcgc
     3121 cgcgggccga ctggcggccg accctgaccg agccgagcgt cgaaccgtcc aggcccaccc
     3181 ggtacagcgt cccctcgacc gacagcgcat agacggagcg ccagccgggc gcccataccg
     3241 gagccgcgtc gaagctgccc tcgagcggca ccgggaagcc gggcgacgcc gccgcgtcga
     3301 tggcgtaggc cagaagctgg cccgcctcgg tcaacgccag cgcgcgaccg tcggccgcgg
     3361 tatccgcccc ggcgaaggcc ggcgccgccg aggcgatacc gggtaacggc acgggccagc
     3421 cgggtagcgc catgccctcg aggtccatca ggtagagctc ggaatcgaac gagcgcggca
     3481 gggccgccag cgcgtatccg gatacggcag gggccgatcg ttgtcgcgag tcgaaggcgc
     3541 tcgagaagcg ggtcgagccg tcggccgata cgagcatcag cgc
//
LOCUS       DAOPHV010000242         3579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_35860_length_3579_cov_3.168275, whole genome shotgun sequence.
ACCESSION   DAOPHV010000242 DAOPHV010000000
VERSION     DAOPHV010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35860_length_3579_cov_3.168275"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLQ29_07290"
     CDS             complement(<1..398)
                     /locus_tag="PLQ29_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86490.1"
                     /translation="MNVRRHALNTLSILAVVAAAVALTSCAEDPAGIFSRVVNEKPIS
                     NNMTKALQYSSPSFVARLGDSYYAGVGVFWAKADGQANWTNEVTLPAAFGSATVLAGS
                     GTVVGGTMYVLFTDSLTGNGLGVWSTANGT"
     gene            complement(414..671)
                     /locus_tag="PLQ29_07295"
     CDS             complement(414..671)
                     /locus_tag="PLQ29_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86491.1"
                     /translation="MAFPIGAFVIAPSAGLGVAISSLGNYKHVDPLFKAGGTFLWKAS
                     SDMSYGLNLYANIIPQIMTEYPENTRVGFFLDATLSVAYHL"
     gene            complement(1132..1686)
                     /locus_tag="PLQ29_07300"
     CDS             complement(1132..1686)
                     /locus_tag="PLQ29_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86492.1"
                     /translation="MGTIETVASIALAMIPPGNVAKELCGVRRAFWTELGAASARAFF
                     DYPVLSWLARPVDGAALAAAAARSRLPFELVGLERFGDDVFIRIPEKYAAEARGLASA
                     FSEAGADTEYRPGPFQAGHGSYCATLAGVGERAAPIVELMPVVAIRAKTYLLAQIELT
                     WAPDESRSSSWATLSSARVNAKPS"
     gene            1741..2511
                     /locus_tag="PLQ29_07305"
     CDS             1741..2511
                     /locus_tag="PLQ29_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HPG86493.1"
                     /translation="MLRKIEFLTERITAVLASWPSVDSVLSMNPSETDAIDPYFALVF
                     DVYYDGEIPSSDERQAMYDNPGAFESSAVVEKDRFFLDGLPIRIEFKKVKILEDFVRN
                     GYDARWAFGTVGTYVIYRVVNGKILFKRTDRLERVLAALKNCPDAFWLRIREAQQSKM
                     EHCLSDLGGAAMKDDRYFYFISLAGFMRACVSALFAINRRFEPSDRQMADSLAELPVL
                     PEDFSGRWSNLLRTDDSVYPQKRYQIAQLITRDVLALE"
     gene            2504..>3579
                     /locus_tag="PLQ29_07310"
     CDS             2504..>3579
                     /locus_tag="PLQ29_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86494.1"
                     /translation="MSRLLRGTRLVIGLAAAAAVIPCSCAAGYVDGLSFFDSDGGASR
                     VASEADGGSSVPAGATAIYRPESGYRAGAIEDLAVFVRGSGAVRVRLLPRGRDPEPLA
                     SVEAALARADESELRLRLSPGMEFSSIEVSALDGGAEVLGFAVLPVFEGYARSGARVT
                     MGHGVVAGAEPGRYFVRFPLAQGDRSSLVASLAGDGAVSLMVIGPDGRERVAYTATVR
                     GGDLLAVPTAGFGAAAAVVVASGSTIASVQTEAGSGAPLSDLHAILSMPAPAGDYSIY
                     RWDVLPRTLVLDFADYDTQDRYLKRLAFFAEKPGFRGRLAADDEIAGLHGWNAHDYPT
                     FTLARFYALAADTGFRLNAEEERFR"
BASE COUNT          620 a         1131 c         1183 g          645 t
ORIGIN      
        1 tcggtaccgt tcgcggtcga ccagacgccg aggccgttgc ctgtcagcga gtctgtgaac
       61 agcacgtaca tcgttccgcc gacgaccgtg ccgctacccg cgagcacggt cgcgctaccg
      121 aaggcggccg gcagggtaac ctcgttcgtc cagttggcct ggccatcggc cttggcccaa
      181 aacacgccaa cgccggcgta gtagctatcg ccaagccgtg cgacgaagga ggggctcgaa
      241 tactggaggg ccttggtcat gttgttcgag atgggcttct cgttgaccac ccgggagaaa
      301 atgcccgcgg ggtcctcggc gcaggaggtc agggcgacgg ccgcggcgac gactgcgagg
      361 atcgatagcg tgtttagcgc atggcgacgt acattcattc ttcgatcctc cgcttatagg
      421 tgataggcga ccgacagcgt cgcgtccaga aagaagccga cgcgcgtgtt ctcgggatac
      481 tcggtcatga tctgcggtat gatgttcgca tacaggttga ggccatagct catatcactc
      541 gacgctttcc agaggaaggt gccgcccgcc ttgaacagcg ggtcgacgtg cttatagttg
      601 ccgagcgagc tgatggcgac gccgaggccg gcgctcggcg ctatgacgaa ggcgccgatg
      661 ggaaaggcca tcacggcccg tagcgccagc ggcgctatga acagctggtc gtcgttgacc
      721 gtacgtatga agtcgccgga caggtccccg gcgaggcccc agcgttcgct cagaaagccg
      781 gtatacgaca gggagaacga aaaaccggcg gctgaattcg gactctcgaa cgcggactcg
      841 gtcggattaa agaagccggg ggtaccgacg tgccgaggga tagcgccagg atgccatcgc
      901 ctcgctcgaa agcgccgaat acggcagagg ccataggcgt ctgaggagcg gcctcctggg
      961 ctgcggcggc gttggtcgcg accagggcgg ctacggccat ggcggccgag aatgcaatcg
     1021 tgtgaaggga acgcatcggt cctccgagtg tatcgcggtt gttacggcgg ggcagactat
     1081 agcacgcgcc cgacgactct gaaaaggcgc gagagcgtcc gtggttaccg gctaggacgg
     1141 tttcgcgttg acgcgggccg acgatagggt ggcccaggac gacgatcggc tttcgtccgg
     1201 cgcccaggtc aactcgatct gggcgagcag gtaggtcttg gctcggatgg cgacgacagg
     1261 catcagctcg acgatgggcg cggcacgctc gccgacgcca gcaagggtcg cgcaatagga
     1321 accgtgcccg gcctggaacg gcccggggcg gtactccgta tcggcgcccg cctcggagaa
     1381 agcggacgcc agcccgcggg cctcggccgc gtatttctcc ggaatacgta tgaagacgtc
     1441 gtccccgaat cgttcgagtc cgaccagctc gaacggcagg cggctcctcg cggccgccgc
     1501 ggcgagagcc gcgccgtcga ccggcctggc cagccacgat agcaccggat agtcgaaaaa
     1561 cgcccgggcg ctggccgcgc cgagctcggt ccagaacgcc ctgcgaacgc cgcatagctc
     1621 cttggccacg ttgcctggcg gtatcatcgc gagcgctatg gaagcgacgg tttctattgt
     1681 tcccaaaggc atgaaggcat agtatactcg gtctatcctc ctgtaaagga aagttcggcc
     1741 atgcttagaa agatagagtt cctgaccgag aggatcaccg cggttctggc ctcctggccg
     1801 agcgttgaca gcgtcctctc gatgaacccg tcggagaccg acgcgatcga cccgtacttc
     1861 gccctcgtct tcgacgtgta ttacgacggc gaaataccgt cgagcgacga gcgccaagcc
     1921 atgtacgaca acccgggagc cttcgagtcg tccgcggtgg tggagaagga tcgcttcttc
     1981 ctcgatggcc tgccgatacg catcgagttc aagaaggtca agatactcga ggatttcgtg
     2041 cgtaacggct acgacgcccg ctgggccttc ggcaccgtcg gcacctacgt catataccgc
     2101 gtcgtcaacg gcaagatcct gttcaagcgg accgaccgcc tcgagcgcgt actggcggcg
     2161 ctgaagaatt gcccggatgc attctggctc aggatccgcg aggctcagca gtccaagatg
     2221 gagcattgcc tgagcgacct gggtggggcc gccatgaagg acgaccgcta cttctatttt
     2281 atatctctgg caggcttcat gcgcgcctgc gtgtctgccc tcttcgccat caaccggcgc
     2341 ttcgagccgt cggacaggca gatggccgat tccctggctg agctgcccgt gttgccggag
     2401 gacttttccg ggcgatggtc gaatctgctc cgtaccgacg attcggtcta tccgcagaag
     2461 cgctaccaga ttgcgcagct catcacgcgt gacgtgttag cccttgagta ggctcctccg
     2521 cggaacccgc ctcgtaatcg gtctcgctgc ggccgcggct gtcataccct gctcctgcgc
     2581 cgccggttac gtcgatggac tgagcttctt cgactcggac ggcggggcgt cgcgcgtcgc
     2641 gagcgaagcg gacggcggct cgagcgtccc ggcgggagcg acggccatct atcggccgga
     2701 atcggggtat agggccgggg ctatcgagga tctagccgtc ttcgtacgcg gttccggcgc
     2761 ggttcgcgtg cggctcctgc cccgaggccg ggatcccgag ccgctcgcgt ccgtcgaggc
     2821 cgcgctagcc cgtgccgacg agagcgaact tcgcctgcgt ctttcgcccg gcatggaatt
     2881 ctcttccatc gaagtctcgg cgctcgatgg cggcgccgag gtcctgggct tcgcggtcct
     2941 tcctgtattc gaaggctacg cgcgctcggg cgcgcgtgtg acgatgggtc atggcgtcgt
     3001 cgcaggcgcg gaaccgggac gatatttcgt tcggttcccc cttgcccaag gtgatcgttc
     3061 ctcgctggta gcgagcctcg ccggcgacgg cgccgtcagc ctgatggtta tcgggccgga
     3121 cggacgggag cgagtcgcct ataccgcgac ggtacgcggc ggcgacctgc tggccgtacc
     3181 tacggccggc ttcggagcgg cggcggccgt cgtcgtcgcg tcaggctcga cgatagcctc
     3241 ggtccagacc gaggcgggca gtggcgcccc gttgtccgat ttgcacgcca ttctgtccat
     3301 gcccgccccg gccggcgatt actcgatcta ccgctgggac gtactgcctc gcaccctcgt
     3361 gctcgatttc gccgattacg atacgcagga tcgatacctg aagcgcctag ccttcttcgc
     3421 cgagaagccg ggcttcaggg gacgcctggc cgccgacgac gagatagccg gtctccatgg
     3481 ctggaacgcc cacgactatc cgaccttcac gctagcgagg ttctacgccc tggccgccga
     3541 taccggcttt cgcctgaacg cggaggagga acgcttccg
//
LOCUS       DAOPHV010000243         3557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_36156_length_3557_cov_3.899772, whole genome shotgun sequence.
ACCESSION   DAOPHV010000243 DAOPHV010000000
VERSION     DAOPHV010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36156_length_3557_cov_3.899772"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="PLQ29_07315"
     CDS             <1..994
                     /locus_tag="PLQ29_07315"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPG86495.1"
                     /translation="EGRSLTLALSSANSGETLLPSTLDPASLCKDKKLDLLVYGSLRV
                     RGGFLAIDLSLYVASLGRAVWTETRYASADGLGQAVAAFSRPLAEAVIGRPYARVGFR
                     VEPPDADLYVDGEAFPKAFALYFEPGRSEVVAKARGYERVGRSFDVSLGEDLFVDLSL
                     EAQGSVGWKLTSEPEGAAIHLDGERVGYAPFDLPGSAFPRLIRVSMPGFEPVQLVHRP
                     EALLDDLTIVLEPDDGLSFDERYDRRKDAFYRSLGWFVSSLPLTVLSGGLFQTYYATA
                     QGYSASGEVDPEVIRRLDSAFYTSQAVFWASAAASIGCAVWAVVRLVAYIEITL"
     gene            1015..1887
                     /gene="ftsY"
                     /locus_tag="PLQ29_07320"
     CDS             1015..1887
                     /gene="ftsY"
                     /locus_tag="PLQ29_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPG86496.1"
                     /translation="MRFGDRIRKLLGMEVVGDDDWDDLADLLVEGDLGAAFADESVSR
                     LKEACATAGVRNGDGARRLLKDVLRPYAVPVDIRPIPGGRNVVMMLGVNGVGKTTSSA
                     KLAKLWLGQGLATKAILAAGDTFRAAAIEQLKAHGDRLGVRVVAQERGSDSGAVLYDA
                     LEAAAADGSDLVIADTAGRMHTKANLIKELEKMDKIVAAKADPSRYKRLLVVDATTGQ
                     NALRQAEAFSAAVKVDGLVLTKYDSSARGGVALAIARQLGIPTAFVCDGERYEDIRAF
                     DPETYLDQFLGLTP"
     gene            1884..3032
                     /locus_tag="PLQ29_07325"
     CDS             1884..3032
                     /locus_tag="PLQ29_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86497.1"
                     /translation="MRRHAFLLMAGLSAALALPLAAQVATGWYVSDPSGLAIEPTAGP
                     GGAEYALLVTGSGGSFRSELYRDGEAVRAWLRTYGRRGAIVREAMEEGGSIREERLYD
                     GDGQPSLERIFLADGSVEETAYEYAAGRLVSRTTSRDGAVVSTTSYLYAPDGRLAMAR
                     ATAGAATGAAAGTAGGRASSAGGASSGWTLGPLGLELRSYDGDGRLVRVALYVGAEEQ
                     RSEERTWAGGRLERVVVTEPSGARTETVYATDGPAAGEIVSLTERRGDVVAHVELRSY
                     DRDGRLILVERSSGGTGASSGGTGASSGAAASTTAYEYDGSGELVSATTTVGGEIVTA
                     VRYEPDGARVEESYDSGELFARVRYEDGRRVLEELLERGQVIQTRSFE"
     gene            3029..>3557
                     /locus_tag="PLQ29_07330"
     CDS             3029..>3557
                     /locus_tag="PLQ29_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG86498.1"
                     /translation="MRRRPWVLFVARRWFGSSDRTGPSLGPATAGIAVGVAALLCVIG
                     VMNGFQMGYIDAVLDLDSYHIRLPSGDAVAVLAALPDAEAAVPFVDLRTLAANRRGKA
                     AALRIKVLPDDALERDRSLAARMELRSGEYGGGVVVGSVLARQLDLRVGDTLSVLDVK
                     ADELDGYLDLFERGWV"
BASE COUNT          532 a         1202 c         1257 g          566 t
ORIGIN      
        1 cgagggccgt agcctgactc tcgccctctc gtcggccaac tccggcgaga cgctcctccc
       61 ctcgacgctc gacccggcct cgctctgcaa ggataagaag ctcgatttgc tcgtctacgg
      121 ctcactacgc gtccgcggcg gcttcctggc catcgacctg tcgctctacg tggcgtcact
      181 cggcagggcg gtctggaccg agacccgcta cgcttccgcc gacggcctcg ggcaggcggt
      241 cgccgccttc tcgaggccgc tcgcggaggc cgtcatcggt cggccctacg ctcgagtcgg
      301 tttcagagtc gaacctcccg acgccgacct ctacgtcgac ggcgaggcct tccccaaggc
      361 cttcgcgctg tacttcgagc cgggtcggag cgaggtcgta gccaaagcca ggggctacga
      421 gcgggtcggc cgctcgttcg acgtgtcgct cggcgaggat ctgttcgtcg acctgagcct
      481 cgaggcccag ggctcggtcg gctggaagct gacgagcgag cccgaagggg cggccatcca
      541 cctcgacggc gagcgcgtcg gctacgcgcc gttcgacctg cctggctcgg ccttccctcg
      601 gctgatacgc gtgagtatgc cgggcttcga gcccgtccag ctcgtccacc ggccggaggc
      661 gctcctcgac gacctgacca tagtccttga accggacgac gggctatcct tcgacgagcg
      721 ctacgatagg cgcaaggacg ccttctaccg atccctcggg tggttcgtgt cgtccctgcc
      781 attgaccgtg ctatcgggcg gcttgttcca gacctactac gcgacggcgc agggctattc
      841 tgcctccggc gaggtcgacc cggaggtgat acgaaggctc gactcggcgt tctatacctc
      901 gcaggcggtc ttctgggcct cggcggccgc ctcgataggc tgcgccgtct gggcggtggt
      961 ccgactcgtc gcgtacatcg agattaccct gtagcgtatg aaaaaggaga catcatgagg
     1021 ttcggcgatc gtatacgcaa gctgctcggc atggaggtcg tcggcgacga cgattgggac
     1081 gacctggccg acctgctcgt cgagggtgac ctcggcgcag ccttcgccga tgagtccgtc
     1141 tcgcggctca aggaggcgtg cgcgacagcg ggcgtcagga acggcgatgg cgcgaggcgc
     1201 ctcctcaagg acgtgctccg gccctacgcc gtgcccgtag acatacggcc gatacccggc
     1261 gggcgcaacg tcgtgatgat gctcggcgtg aacggcgtcg gcaagacgac gagctcggcg
     1321 aagctggcca agctgtggct cggccagggg ctggccacga aggccatact ggcggccgga
     1381 gacaccttca gggccgcggc catcgagcaa ctcaaggctc atggcgaccg gctcggcgtc
     1441 agggtcgtcg cccaggagcg cggctccgac tcgggcgcgg tgctctacga tgccctcgag
     1501 gccgcggcag ccgacggctc cgacctggtg atagccgata ccgccggccg gatgcatacc
     1561 aaggccaacc tgatcaagga actcgagaag atggataaga tcgtcgcagc caaggccgat
     1621 ccgtccaggt acaagcgcct cctcgtcgtc gacgcgacga ccggccagaa cgcgctcagg
     1681 caggccgagg ccttctcggc ggccgtcaag gtggatgggc tcgtcctaac caaatacgac
     1741 tcgagcgcca ggggcggcgt ggccctggcc atcgccagac agctcggcat cccgacggcc
     1801 ttcgtctgcg acggcgagcg ctacgaggac atacgggcct tcgatcccga gacctacctc
     1861 gaccaattcc tgggactgac gccttgagac gacatgcctt cctcctcatg gccgggttat
     1921 cggccgcctt ggcgctgccg ctcgcggccc aggtcgctac cggatggtac gtctccgacc
     1981 cgtcgggcct ggccatcgag ccgaccgccg ggccaggcgg tgcggagtac gcgctcctcg
     2041 tcacgggctc gggcggctcg ttccgctcgg agctgtatcg cgacggcgag gcggtccggg
     2101 cctggctccg gacctacggc cggcgcggtg ccatcgtccg cgaggccatg gaggaaggcg
     2161 gctcgatacg cgaggaacgg ctgtacgacg gagacgggca gccgagcctc gagcgtatct
     2221 tcctggccga cggctcggtc gaggagacgg cttacgagta cgcggccgga aggctcgtgt
     2281 cgaggacgac gagccgcgac ggagccgtcg tatcgacgac gagctacctg tacgcgccgg
     2341 acggtcggct ggctatggcc cgagcgacgg caggcgccgc cacaggcgcc gccgccggta
     2401 ccgccggcgg tagagcgtct tcagccggcg gagcgtcgtc gggctggacg ctcgggccgc
     2461 tcggactcga gctacggtcc tacgacggcg acggtcggct agtcagggtg gcgctctacg
     2521 tcggcgccga ggagcaacgg agtgaggagc gtacctgggc cggcggccga ctcgagcgcg
     2581 tcgtcgttac cgagccgtcg ggagcgcgga cagagaccgt ctacgctacc gacgggccag
     2641 ccgccgggga gatcgtatcg ctgaccgaac gccgcggcga cgtcgtggct cacgtcgagc
     2701 tacgctccta cgaccgcgac ggtcggctga tcctcgtcga gcggtcgtcc ggcggtaccg
     2761 gcgcgtcgtc cggcggtacc ggagcgtcgt ccggcgctgc ggcgtcgacg accgcctacg
     2821 agtacgacgg ctcgggcgag ctggtctcgg ccaccaccac ggtcggcggc gagatcgtca
     2881 cggcggttcg ttacgagccg gacggcgcac gcgtcgagga gtcgtacgac tcaggcgagc
     2941 tattcgcccg cgtacgctac gaggacggcc ggcgcgtgct cgaggagctg ctcgagcgcg
     3001 gccaggtgat ccagacgagg agcttcgagt gaggcgacga ccgtgggtcc tgttcgtggc
     3061 caggcgctgg ttcggctcga gcgacaggac cggcccgtcc ctggggccgg cgaccgctgg
     3121 catagccgtc ggcgtcgccg cgctcctctg cgttatcgga gtgatgaacg gcttccagat
     3181 gggttacatc gacgcggtgc tcgacctcga cagctaccat atacggctac cgtccggcga
     3241 cgccgtggcg gtgttggccg cgctaccgga cgctgaggcg gccgtgccct tcgtcgacct
     3301 gcggaccctg gcagccaacc gtcgcggcaa ggcggccgcc ctgcgtatca aggtgttgcc
     3361 cgacgacgcg ctcgagcgcg accgctcgct ggccgccagg atggagttgc gttccggcga
     3421 gtacggcggc ggcgtcgtcg tcggctcggt gctcgccagg cagctcgacc tccgggtggg
     3481 cgatacgctg tcggtcctcg acgtgaaggc cgacgagctc gacggctatc tggacttgtt
     3541 cgagcggggt tgggtcg
//
LOCUS       DAOPHV010000244         3547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_36286_length_3547_cov_4.964490, whole genome shotgun sequence.
ACCESSION   DAOPHV010000244 DAOPHV010000000
VERSION     DAOPHV010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36286_length_3547_cov_4.964490"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLQ29_07335"
     CDS             <1..576
                     /locus_tag="PLQ29_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86499.1"
                     /translation="CSLNGIRVGALFFGGATAMDGRAPASGQGAARALMERLAAGPAS
                     AAGPASAAGPARFGSELGAALTAASSILKRRSLVLVVSDFMCSGYALPLALLARRNDV
                     AALRVHDRLDSAPPALGLTLRSADAESGERRLVTPRSAAYRRERERWAKAARLEWLVA
                     LSAAKVPYLEMDSSTDPVEELIAFFGRRRSA"
     gene            576..1508
                     /locus_tag="PLQ29_07340"
     CDS             576..1508
                     /locus_tag="PLQ29_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86500.1"
                     /translation="MGVIGRRPRRRVGGPLVGAALAVALLAAAPTGARAEAAEASILR
                     VEFDPPTFYPGQATLAYATLDPGTASWSEAVLAVVSERPDEAGAPETGAPEVEILSAR
                     LERRGGQPILIVRFVPWTAGRGLIPGMELGGLAVTGLRFDCASALTSLRPPVLLPQLE
                     PPGLRSRLYLAGGVALVALLAGLTAAFRAAPWFGAIKARWAAARSRKEFDALLEAVVE
                     IEPETAAWAALCAAVRRFVGTRTGVDWSAMTASEIALSPEGPVPGPTRDAAAAILSSG
                     DAARFAGSVEAGLADAVAAARAVADSLDEAGRAP"
     gene            1505..2551
                     /locus_tag="PLQ29_07345"
     CDS             1505..2551
                     /locus_tag="PLQ29_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPG86501.1"
                     /translation="MIAFDHPLALALCLAPVAAAVAGARGRNRSRLVVLPLDVWGGAI
                     SAEAPAPWRAARAASALLFALAWMALSVAAAGPASVAESAGRANAGADVVFVLDASPS
                     MAARDLEPTRLEAAKAFVLSWLGSPEGASGAAVGLVAFGAEAALACPPTVDYAVVAER
                     LALIRPGILGDGTAIGQGLASAYRQVKASGAARALVVLLSDGEDNVGLVHPADAARAV
                     AASGAALLVVGLGSRGDVPIDYVDPDSGQRMSGAYRSGFDDAALAAVAAAGGGEYRSA
                     GDAKSLEALAATLGDSVSGLAPSERSEDGGRVSAPGPADSSRRPLGRALVLAAMALAA
                     VGWALRALALGGLA"
     gene            2548..>3547
                     /locus_tag="PLQ29_07350"
     CDS             2548..>3547
                     /locus_tag="PLQ29_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPG86502.1"
                     /translation="MSGLAPWAVAALVPYAALQAAYLRRLATVPEALGGTMEANGRAR
                     WRRYVARRAVGAAFGAVFWVLCALAASGAVRVPRYATQTVTGAELAFVLDASNSMLSA
                     DEGVSRLERASDFVSRLAAAADGAGLSVIAFRGRPVTLCPSTRDRKAFADALLWAGPA
                     VTSAAGSDVGAAIDEALRPAFPPGAARLVIVLSDGNDTGGRAKAAAAEAAASGAALLV
                     VGFGGDEPAIVVDPDGKPVVGDGGTPVKKAIDEAAMRDWAVAGRGEYLRASDPASLGR
                     AAAICASTSSSSGSRRTVRVEVDASSALALAALAALALAALASWPALEPRRPPLRRK"
BASE COUNT          364 a         1353 c         1334 g          496 t
ORIGIN      
        1 tgctcgctca acggcatcag ggtgggcgcg ctattcttcg gcggcgccac cgcgatggac
       61 ggccgcgcgc cggcctcggg ccagggagcc gcccgggcgc tcatggaacg tctcgccgcg
      121 gggccggcga gcgccgcggg gccggcgagc gccgcggggc cggcgcggtt cgggtcagaa
      181 ctcggcgcgg ccctgaccgc cgcctcgtcc atcctgaaac gccgtagcct cgtcctcgtc
      241 gtctcggact tcatgtgctc gggctacgcc ctgcccctgg ccctgctggc caggcgcaac
      301 gacgtcgcgg ccctgcgcgt gcacgaccgc ctggactcgg ccccgcccgc cctcggcctg
      361 acgctgcgat cggccgacgc cgagtcgggc gagcggcgcc tcgtgacgcc ccgctcggcc
      421 gcgtaccgcc gcgagcggga gcgatgggcc aaggccgccc ggctcgagtg gctcgtggcg
      481 ctgtccgcgg ccaaggtgcc gtatctcgag atggactcgt ccaccgaccc ggtcgaggag
      541 ctcatcgcct ttttcggccg gaggaggagc gcctgatggg cgtcatcggg cggcgccccc
      601 gccgccgcgt cggcggcccg ctcgtgggcg cggcgctcgc cgtagccctc ctggccgccg
      661 cgccgacggg cgctcgcgcc gaggcggcgg aggcttctat cctgagagtc gagttcgacc
      721 ctcctacctt ctaccccggg caggcgacgc tggcctacgc gaccctcgat cctggaacgg
      781 cttcctggag cgaggccgtc ctggccgtcg tctccgagcg gcccgacgag gccggggctc
      841 ccgagaccgg ggctcccgag gtcgagatcc tgtcggcccg gctcgagcgt cgaggcggcc
      901 agccgatact gatcgtccgc ttcgtcccgt ggacggcggg ccggggcctc atcccgggca
      961 tggagcttgg cggcctcgcc gtgacgggcc tacgcttcga ctgcgccagc gccctgacca
     1021 gcctgcggcc tcccgtcttg ctgccccagc tcgagcctcc ggggctccgc tcgcggctct
     1081 acctcgccgg aggcgtcgcc ctggtcgcgc tgctcgccgg cctgaccgcg gccttccgcg
     1141 ccgcgccctg gttcggcgcc atcaaggcgc gatgggccgc cgcgaggagc cggaaggaat
     1201 tcgacgctct actggaagcg gtcgtcgaga tcgagccgga gacggccgcc tgggccgcgc
     1261 tctgcgccgc cgtcaggcgt ttcgtcggga ccaggaccgg cgtcgactgg agcgccatga
     1321 cggcgtccga gatagcgctc tccccggagg gccccgtgcc gggtcctacc cgcgacgccg
     1381 cggccgcgat cctttcgtcc ggggacgccg cccgtttcgc gggctcggtc gaagccggcc
     1441 tggctgacgc cgtcgcagcg gcccgcgcgg tcgcggacag cctcgacgag gcggggaggg
     1501 cgccgtgata gccttcgacc atccgctcgc gcttgcgctg tgcctggccc cggtggccgc
     1561 cgccgtggcg ggggcccgag ggcggaaccg gagccgtctc gtagtcttgc cgctcgacgt
     1621 ctggggcggc gccatcagcg ccgaagcgcc tgcgccttgg cgagccgcgc gcgcggcctc
     1681 ggcgctgctg ttcgccctgg cctggatggc gctgtcggta gccgccgccg gcccggcctc
     1741 cgtcgcggaa tcggccggcc gcgccaacgc cggagccgac gtggtgttcg tgctcgacgc
     1801 ttcgccgagc atggccgccc gagacctcga gccgaccagg ctggaggccg ccaaggcttt
     1861 cgtgctgtcc tggctcggtt cgcctgaggg cgcgtcgggg gccgcggtcg gcctggtggc
     1921 gttcggggcc gaggccgcgc tcgcctgccc gccgaccgtc gactacgcgg tcgtggccga
     1981 gcggctcgcc ctgatacggc caggcatcct cggcgacggc accgccatcg gccaggggct
     2041 ggcgagcgcg taccgccagg taaaagcctc gggtgccgcg cgcgccctcg tggtgctcct
     2101 gagcgacggg gaggacaacg tcggcctcgt gcatccggcc gacgcggcgc gggccgtcgc
     2161 cgcctccggg gccgcgctgc tcgtggtcgg cctaggctcc cgcggcgacg tgccgatcga
     2221 ctacgtcgat ccggacagcg gccaacgcat gagcggcgcc tatcgctccg gcttcgacga
     2281 cgcggcgctg gcggccgtcg ccgccgccgg cggcggcgag taccgctcgg ctggagacgc
     2341 gaaaagcctc gaggccttgg cggcgacgct cggcgattcc gtgtccgggc tcgcgccgag
     2401 cgagcgctcc gaggacggcg gccgggtctc cgcgccggga ccggccgact cgtcgcgccg
     2461 gccgctgggc cgcgccctgg tactggccgc gatggccttg gccgccgtcg gctgggcgct
     2521 acgcgccctg gcgctcgggg ggctcgcgtg agcggcctcg cgccctgggc agtcgccgcg
     2581 ctggttccgt acgcggccct gcaggccgcg tacctgaggc ggctcgccac ggtcccggag
     2641 gccctaggcg gtaccatgga ggcgaacggt cgcgcccgct ggcgtcgcta cgtggcgcga
     2701 cgggccgtcg gcgcggcttt cggggcggtc ttctgggtgc tatgcgcctt ggccgcctcc
     2761 ggggcggtac gcgtgccgcg ctacgccacc cagacggtga ccggggccga gctggccttc
     2821 gtcctcgacg cgtccaactc gatgctgagc gccgacgagg gagtgagccg gcttgagcgg
     2881 gccagcgatt tcgtctcgag actggcggcc gccgccgacg gcgccggcct gtcggtgata
     2941 gcgttcaggg gccgtccggt caccctctgc ccgtccaccc gcgatcgcaa ggccttcgcc
     3001 gacgccctcc tttgggccgg ccccgcggtg acgagcgcgg ccggttcgga cgtcggcgcc
     3061 gcgatagacg aggcgctcag gcccgccttc cctcccggcg ctgccaggct cgtgatcgtc
     3121 ctcagcgacg ggaacgatac cggcggacgc gccaaggccg cggccgccga agccgcggct
     3181 tccggggcgg ccctcctcgt cgtcggcttc ggcggcgacg agccggccat cgtcgtcgac
     3241 ccggatggca agcccgtggt cggcgacggc ggaacgcccg tcaagaaagc catagacgag
     3301 gccgcgatgc gcgactgggc cgtcgcggga cgcggcgaat acctgcgggc gtcggatccg
     3361 gcttcgctcg gccgcgcggc cgccatctgc gcgtccacct cctcgtcgag cgggtcgagg
     3421 aggaccgtgc gcgtcgaggt cgacgcgtcg tcggccctgg ccctagccgc gctcgcggct
     3481 ctggccctgg ccgccctggc gtcctggccg gcgctcgagc cgcgacgacc gccgctccgc
     3541 cgaaagg
//
LOCUS       DAOPHV010000245         3529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_36535_length_3529_cov_3.460852, whole genome shotgun sequence.
ACCESSION   DAOPHV010000245 DAOPHV010000000
VERSION     DAOPHV010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36535_length_3529_cov_3.460852"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PLQ29_07355"
     CDS             complement(<1..477)
                     /locus_tag="PLQ29_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86503.1"
                     /translation="MERSIEEVCEAILRIELVPALGCTEPIAIAYAAAVARETLGVFP
                     ERMTIRCSGNVVKNVKGVVVPGTGDLRGVEASAILGAVGGEASRRLEVLSGLRPEQLE
                     RTRALLAARLCRAELVPDVEGLLIIAELESGGSSSAMMRSPSTSGTSSALMSRGSSS"
     gene            591..1463
                     /locus_tag="PLQ29_07360"
     CDS             591..1463
                     /locus_tag="PLQ29_07360"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPG86504.1"
                     /translation="MPETYTIASFNAENFSMLLDADYGAAEFHALSDERYAAMNPSIF
                     NPNKDRAKASAIASTILERDFDVVGLCEIGGMETLENFNRYYLGGRYECFLHEANSRR
                     GIFVGALVKRGRFDRIEAKPVDGPFSRNVLELRLASGDDRFRLIVVHLKSQSGQDRGI
                     PQRVAEVRRLVEVAAPPACVVMGDFNGILIRGEAEFEYEPFLELPFRDVLEATGVPPG
                     ARFTHFYFRGAEPSFSQLDYIFCSDDVEVADGGVLAELVPLNYEQRRRLPSDHAFIWA
                     AIRPWPVVAPDGQT"
     gene            complement(1454..2494)
                     /locus_tag="PLQ29_07365"
     CDS             complement(1454..2494)
                     /locus_tag="PLQ29_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HPG86505.1"
                     /translation="MSRGTGCRYGSHRVLEPKGILPQPALRLDNDMAIYDNEVLIDVS
                     TLNIDSASYTQIRSSCGADEGRMREAILSIVAERGKMQNPVTGSGGMLIGMVREIGPE
                     YPNKQLKVGDRIATLVSLSLTPLRIASIRRLDPDSDQVDVDAQAIMFESGIYANVPTD
                     LPEKLALAVLDVAGAPAQVARIVEKGDTVCIIGGGGKSGVLCCYQAMKNVGPYGKVIV
                     VEYSREHATRIERMGLATHVIVADATDALDVYRKVTDITGPRGCDVTVNNVNVPNTEM
                     TSILITKGQGLIYFFSMATSFTKAALGAEGVGKDIELIVGNGYTKGHSDLALDILRES
                     ADIRALFEKLYV"
     gene            complement(2571..3209)
                     /locus_tag="PLQ29_07370"
     CDS             complement(2571..3209)
                     /locus_tag="PLQ29_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86506.1"
                     /translation="MKTTTRIASALVAALTCLTCLSCDLLGGKAVYLNAQAMEIFHDL
                     IAQSYDIAPKAYVLEDVSETYSGEESDEVKVAGSARLYGDYDYAWDDYYIDANSHKEV
                     RVWAYDGLEFRYAGYKRSSGNVTIVSGSLTIDGTRRMETRYTSTGYTSYSSRTESGSI
                     RVYGTVVVEGVSEGEEVRDELTVDVTRSLSGTSWYYNGTIENRVESWDVYME"
BASE COUNT          586 a         1183 c         1080 g          680 t
ORIGIN      
        1 tgatgaggag cccctcgaca tcagggccga gctcgtgccg gatgtcgagg ggctcctcat
       61 catcgccgag ctcgagccgc ccgattccag ctcggcgatg atgaggagcc cctcgacatc
      121 cggcacgagc tcggccctgc agagccgcgc ggccagcagg gcgcgcgtac gctcaagctg
      181 ctccggacga aggccggaca ggacctcgag gcgtcgggat gcctcgccgc cgaccgcgcc
      241 gagtatcgcg ctcgcctcca cgccgcgcag gtcgccggtg cccggcacga cgacgccctt
      301 gacgttcttg accacgttgc ccgaacagcg tatcgtcatg cgctccggga aaacgccgag
      361 cgtctcgcga gccacggccg cggcgtaggc tatagctatc ggctcggtgc agccgagagc
      421 cggcaccagt tctatcctca ggatggcctc gcagacctcc tcgatgctcc gttccatgaa
      481 gactcctatt ggcgacagga tagcgattcc aggcgccggc cgcaaggcgc tatcgtccgc
      541 cgcgggcgtt ccggtatagg atggcccgat agcccgaacc aggaggccca gtgcccgaga
      601 cctacacgat agcgtcgttc aatgcggaga atttctcgat gctactggac gccgactacg
      661 gtgccgccga gttccacgcc ctctcggacg agcgctacgc cgcgatgaac ccgtcgatct
      721 tcaacccgaa caaggaccgg gccaaagcgt cggcgatagc ctccacgatc ctggaacgag
      781 acttcgacgt ggtcgggcta tgcgaaatag gcggcatgga gacgctcgag aatttcaacc
      841 gctattacct cggcgggcgc tacgagtgct tcttgcacga ggccaacagc cgtcgcggca
      901 tcttcgtggg agccctggtc aagcgcggcc gcttcgaccg catcgaggcg aagcccgtcg
      961 acggcccctt ctcgcgcaat gtgctcgaac tgcgcctggc ctccggcgac gacaggttcc
     1021 gccttatagt ggtgcacctc aagtcgcagt cggggcagga ccgcggcata ccccagcgcg
     1081 tcgccgaggt ccgcaggctc gtcgaggttg ccgccccgcc cgcctgcgtc gtaatgggcg
     1141 acttcaacgg catcctgata cgcggcgagg cggagttcga gtacgagccg ttcctggagc
     1201 taccgttccg cgacgtgctc gaggcgacgg gcgtaccgcc gggcgcccgc ttcacccatt
     1261 tttacttccg gggagccgaa ccgagcttca gccagctcga ctatatcttc tgctcggacg
     1321 acgtcgaggt ggctgatggc ggcgtcctcg cggagctcgt gccgctcaac tacgagcagc
     1381 gacggcgcct accgtccgat cacgccttca tctgggcggc gatacgaccg tggcccgtcg
     1441 tcgccccgga cggtcagacg tagagctttt cgaacagcgc cctgatatct gccgactcgc
     1501 gcaggatatc gagggccagg tccgagtgcc ccttggtgta cccgttgccg acgatcagct
     1561 cgatgtcctt gcccacgccc tctgcgccga gggccgcctt ggtgaacgag gtggccatcg
     1621 agaagaagta gatgaggccc tggcccttgg tgatcaggat gctcgtcatc tccgtgttcg
     1681 gcacgttgac gttgttgacc gtcacgtcgc agcctcgggg accggtgatg tcggtcacct
     1741 tgcggtacac gtccagggcg tcggtcgcgt cggctacgat cacgtgcgtg gccaggccca
     1801 tccgctctat cctggtggcg tgctccctgg agtactcgac gacgatgacc ttgccgtagg
     1861 gcccgacgtt cttcatagcc tggtagcagc acagcacgcc ggacttgcct cctccgccga
     1921 tgatgcagac ggtgtcgccc ttctcgacga tgcgggctac ctgggccggg gcgccggcga
     1981 cgtcgagcac cgcgagcgcc agcttctcgg gcaggtccgt cggcacgttg gcgtagatgc
     2041 cgctctcgaa catgatggcc tgggcgtcga cgtcgacctg gtcggagtcc gggtccagcc
     2101 tcctgatcga ggctatcctg agcggcgtca gagacagcga cacgagggtg gctatcctgt
     2161 cgccgacctt cagctgcttg ttcgggtact cgggccctat ctcgcgcacc atgcctatca
     2221 gcatgccgcc agagccggtc accgggttct gcatcttgcc ccgctccgcc acgatggaca
     2281 ggatggcctc cctcattcga ccctcatcag ccccgcagga cgaacgtatc tgcgtatagc
     2341 tagccgagtc tatgttgagc gtactgacgt cgatgagcac ctcgttgtca tagatggcca
     2401 tatcgttatc gagcctgagc gccggctgcg gcagtatccc cttcggttcg agcaccctat
     2461 ggctaccgta cctgcatccc gttcccctgg acatatcact acctcctcgc gacgggcgga
     2521 atcgtatcat cccggggcgc gatgcaggaa gatgtcggcg ccgaccccgg tcattccatg
     2581 taaacgtccc atgattcaac gcggttttct atcgtcccgt tgtagtacca gcttgtgccg
     2641 ctcaggctac gcgtcacgtc gaccgtcagc tcgtcgcgaa cctcctcgcc ctcggagacg
     2701 ccctcgacga cgaccgtgcc gtagacgcgg atcgaaccgc tctcggtccg gctcgagtat
     2761 gaggtatagc cggtggaggt gtagcgggtc tccatgcgcc tggttccgtc aatcgtcaac
     2821 gagccggaga ctatcgtgac gttcccgctc gagcgtttat agcctgcata gcggaactcg
     2881 agcccatcgt aggcccagac gcgtacttcc ttatgggaat tcgcgtcgat atagtagtcg
     2941 tcccaggcgt agtcataatc cccgtacaat cttgccgagc cggccacctt gacttcgtcc
     3001 gactcctcgc ccgagtacgt ttccgatacg tcctcgagga cgtacgcctt cggggctata
     3061 tcgtagctct gcgctatcag gtcgtggaag atttccatag cctgggcgtt gagatagacc
     3121 gccttgccgc cgagcaggtc gcacgaaagg caggtcaggc aggtcagagc cgctacgagc
     3181 gccgatgcga ttctggtcgt cgttttcata gagtgtacct ccataggcgc gtgatgatcg
     3241 gtggtacgcc gctcatgaaa ctactggtgc gaggctgatg ctctacgtct aagtatgccg
     3301 cgcgccgaga agctatcaag gctctacggt tctttcgtgt ccgcggctca tcccctctgt
     3361 cagccgatag gctcgacgct cgcagagcca gtccgagcac gcggagcatc cgcgccagga
     3421 ccgattcggc ctccctccgg aaggcctcgc gcgcgccggc cctcctatgg cgattcgcct
     3481 gcgtcgtcgc ctgcgtcgtc gcctgcgtcg tcgcctgcgt cgtcgcctg
//
LOCUS       DAOPHV010000246         3480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_37250_length_3480_cov_4.994745, whole genome shotgun sequence.
ACCESSION   DAOPHV010000246 DAOPHV010000000
VERSION     DAOPHV010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37250_length_3480_cov_4.994745"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PLQ29_07375"
     CDS             complement(<1..1010)
                     /locus_tag="PLQ29_07375"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG86507.1"
                     /translation="MAHTGNEAALTGTGAYDCVVVGAGLGGLCAAFELSRAGAKTLLL
                     EGHNLPGGFATSFVRGRFEFEPSLHELPDMRSMSEATGVVRYLLDDAGLEVGFASVPE
                     AYRLILTDDGVDLRMPFGIEACIAEVERAVPGSRPSVSAYFELCREVQDGFGYLNAHA
                     RSPNVLKLLRDHGDFVRTASATAAEVARAVGVPSRAHDILFAYWCYLGVPAETLSFPI
                     WASLLYSYLSSGAVIPTMRSHELASAFDAAIRRHGGEIRYNSRVDRVLVEDGAAWGVT
                     TASGETIRAAEVVCNASPTTVFESLVHPASERPAAALRAVNARKHGFSVVVVYLGLDA
                     DR"
     gene            1445..1864
                     /locus_tag="PLQ29_07380"
     CDS             1445..1864
                     /locus_tag="PLQ29_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPG86508.1"
                     /translation="MVAYLDSSVMLRYILLGETALRHVQAFPRLASSELLEIECRRVL
                     LRCRIEGELNDESLVEATSRLDEALSGVDLLELDAAVKKRAMEAFPIHVKTLDALHLA
                     SALQLLAEEPADGIVVFSHDRTFNLCARALGFNAALL"
     gene            1947..2435
                     /locus_tag="PLQ29_07385"
     CDS             1947..2435
                     /locus_tag="PLQ29_07385"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPG86509.1"
                     /translation="MSSCQFASLDAIVPAPAFIREAAYAEAERYLGMEYEWGGQDLPR
                     GIDCSGLVVNVYHTVTAGTEYAPPFDDATAAMLSERYTVDIDVPARGDLVFMGDGAVS
                     HVAILDRLGFDENGDERVYFIDAYSAAPGGGRVMERSYPADSPVILSYGRLLVIRHGQ
                     VR"
     gene            complement(2551..>3480)
                     /gene="fusA"
                     /locus_tag="PLQ29_07390"
     CDS             complement(2551..>3480)
                     /gene="fusA"
                     /locus_tag="PLQ29_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684885.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPG86510.1"
                     /translation="AGDIAVFVGMKAAQTGDTVGSEGWPMLLEHMHFPEPVITVAIEP
                     KTASDRDKLKETLDILSKEDPTFQTGDDEDTGQLVIRGMGELHLDVLVTRILREFKVG
                     AKVGNPQVSYRESITRKVEHAERYERTMAGKDVAAGVTLAVEPLERGSGNVYEKAVRG
                     GKVPEEIFDAIERAVNNAFGGGIVLGYPCVDIGVKLVGIDYDELSATPFAFEACASMA
                     FDEACREAGPVQLEPVMKVDIMTPKDYLGDVMSLLSQRGGIINGSDSRAAIDVVHAQA
                     PLSVMFGFSTSLRSVSQGRASFSMEFSHFEQKR"
BASE COUNT          586 a         1096 c         1157 g          641 t
ORIGIN      
        1 tcccggtcgg cgtccaggcc caggtagacg acgacgaccg agaagccgtg cttcctggcg
       61 ttcacggccc gcagagccgc ggccgggcgc tcggaggccg ggtgcacgag cgactcgaag
      121 accgtcgtcg gcgaggcgtt gcagacgacc tcggcggccc gtatcgtctc gcccgaggcc
      181 gtcgtcacgc cccatgcggc gccgtcctcg accaggacgc ggtccactcg gctattgtag
      241 cggatctccc cgccgtgtcg acgtatggcg gcgtcgaagg cgctcgccag ctcgtgcgag
      301 cgcatcgtcg gtatgaccgc tccagacgac aggtacgagt acaatagcga cgcccagatc
      361 gggaacgata gcgtttcggc cggcacgccg aggtagcacc agtaggcgaa caggatgtcg
      421 tgggcccggc tcggcacgcc gaccgccctg gccacctcgg ccgccgtcgc ggaggccgtg
      481 cgcacgaagt cgccgtggtc gcgtagcagc ttgaggacgt tcggcgagcg ggcgtgcgcg
      541 ttcaggtagc cgaagccgtc ctgaacctcg cggcatagct cgaagtacgc gctcaccgac
      601 gggcggctac ccggcaccgc gcgttcgacc tcggctatgc aggcctcgat gccgaagggc
      661 atgcgcaggt cgaccccgtc gtcggtcagg atgagcctat aggcctcggg cacgctcgcg
      721 aagcctacct cgaggccggc gtcgtcgagc aggtagcgca cgaccccggt cgcctcggac
      781 atggagcgca tatcgggcag ctcgtgcagc gacggctcga actcgaagcg gccgcggacg
      841 aagctcgtgg cgaagccgcc gggcaggttg tgcccttcga ggagtagcgt cttggctccg
      901 gcgcgcgaca gctcgaacgc cgcgcacagg cctccgaggc ccgccccgac gacgacgcag
      961 tcgtaggcgc ccgtacccgt cagggcggcc tcgtttcccg tatgtgccat cgggacaagt
     1021 atacacgcta tagggattgc cgcaattcta atcgaggctc ggctcctcgt cgactcgccg
     1081 cgaggcctgg cgcgggacag cgaccgccgg ttagcgttcg ttacgtgcca tcgggattgc
     1141 cttcaatctc cttgactgtt ttgaaatata gtcataatat agtcatataa ggagccttta
     1201 tgcagacagg gaggactgta gccgttgctg aactcaaagc tcatctgagc gccgagctac
     1261 ggcgcgttca gggaggcgag acgaccgtca tcgtggatca cagaaaaccc atagctctat
     1321 tgtccggctt ggatccaatg ccgaagtacg cgcgtagaag acgcgggcct ttcgtctggc
     1381 gagagttcga acccctgact acaagcgcca tacaggcgct gatcgataag gaacgggcgg
     1441 attcgtggta gcgtacctgg attcgtcggt catgctcagg tatatactgc ttggagaaac
     1501 ggcgcttcgt catgtccagg cctttccgcg gctagcttca agcgagctgc ttgagatcga
     1561 atgccgaaga gtcctgttgc ggtgccggat cgagggcgaa ctgaatgacg agtcactggt
     1621 cgaggctacg agtcgcttgg atgaggcgct gagtggtgtc gaccttctag aactcgatgc
     1681 cgctgtcaag aagcgggcta tggaagcttt cccgattcat gtcaaaacgc tcgacgcgct
     1741 gcacctggcg agcgccctgc agttgctcgc cgaagaaccc gcggatggga tcgtggtttt
     1801 ctcgcacgat cgcacgttca acctttgcgc ccgagcgctc ggcttcaatg cggcattact
     1861 ttgatagtga tacaggacgg gccgataaaa cgacgggatg ccggagggcc gtcgccgtat
     1921 gctccacgct ctgtacggct ctgctgttgt cgtcttgtca gttcgcttcg ctcgatgcca
     1981 tcgtcccggc gcccgccttc atacgcgagg cggcttacgc ggaggccgaa cgctacctgg
     2041 gcatggagta cgagtggggc gggcaggatt tacctcgcgg tatagactgc tccggcctcg
     2101 tcgtcaacgt ataccatacc gtaacggccg gcacggagta cgcgccgcca ttcgacgacg
     2161 ccactgccgc gatgctcagc gagcggtata ccgtagatat cgatgtgccg gcccgcggag
     2221 acctcgtgtt catgggcgac ggcgccgtca gccacgtggc catcctggat cgcctagggt
     2281 ttgatgagaa cggcgacgag cgcgtctact tcatagacgc ctactccgca gccccgggcg
     2341 gcggccgcgt gatggagcgt agctacccgg ccgattcgcc tgttattctg tcgtacggtc
     2401 gtttactggt catccggcat ggccaagtcc gctagcgcgg acttggccgg tccgtggcgc
     2461 gtcgtcgtcc gtgacgttcc cgtccgctcg ggcggacggg ccgtgctgtg gcgcgccttc
     2521 cttggcgcgc gatactgtaa tgggcctcgc ttaccttttc tgctcgaaat gcgagaactc
     2581 catcgagaag ctagcccgtc cctgtgatac ggagcggagg ctggtggaga agccgaacat
     2641 gaccgatagc ggcgcctggg cgtggacgac gtcgatcgcg gcgcgggaat cggagccgtt
     2701 gatgatgccg ccgcgctggg acaggaggct catcacgtcg cctaagtagt ccttgggcgt
     2761 catgatgtcc accttcatga ccggctcgag ctggaccggg ccggcttcac ggcaggcctc
     2821 gtcgaaggcc atggaggcgc aggcctcgaa ggcgaagggc gtggccgaca gctcatcgta
     2881 gtcgataccg accagcttga cgccgatgtc gacgcagggg tagccgagga cgatgccgcc
     2941 gccgaaggcg ttattcacgg cccgctcgat cgcgtcgaag atctcctcgg gcaccttgcc
     3001 gccgcggacg gctttctcgt agacgttgcc cgagccgcgc tcgagcggct cgaccgcgag
     3061 cgtcacgccg gccgcgacgt ccttgccggc catcgttcgc tcgtagcgct ccgcgtgctc
     3121 gaccttcctg gtgatcgact cgcggtacga cacctgaggg ttgccgacct tggcgccgac
     3181 cttgaattcc cggaggatgc gcgtgaccag cacgtccagg tgcagctcgc ccatgcctcg
     3241 gatgacgagc tggcccgtgt cctcgtcgtc accggtctgg aaggtcgggt cctccttgga
     3301 caggatgtcg agcgtctcct tgagcttgtc gcggtccgag gcggtcttgg gttcgatggc
     3361 cacggtgatg acgggctcgg ggaagtgcat gtgctcgagc agcatgggcc agccctcgga
     3421 gccgacggtg tcgccggtct gggccgcctt catgccgacg aagaccgcga tgtcgccggc
//
LOCUS       DAOPHV010000247         3470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_37418_length_3470_cov_3.912445, whole genome shotgun sequence.
ACCESSION   DAOPHV010000247 DAOPHV010000000
VERSION     DAOPHV010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37418_length_3470_cov_3.912445"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="PLQ29_07395"
     CDS             complement(<1..1035)
                     /locus_tag="PLQ29_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86511.1"
                     /translation="MSEEIDPEIAALIGGGIEEYSAKPAGRVPGAGGPSFEALFGDMG
                     VAGDVEAKGEFDVDLSKKAYDAVERFESDPPNAFFDDPQYYQKALAGEGEASQRFHEL
                     LKKYLQATDPKDRGMYRQQVITAYWNMVSRMIPRVVSANPIVPKQLMIRFGLSLPTMI
                     SAEQRAIFSRIIHQKTTSEPVYYLDEWLRSIAMGQVNPSSTDEVSQARGDERARINTL
                     LQKAMGKRDTAEALLKSKAEERKSLEGMLRDRIDIICEHAGLPGYYQVPAPYSDAQKK
                     TIAELGEIMRRMLAADKELVSSAEAFEEAGKDVQTIRDKAGALGEDTKANLQALSQEF
                     DTARQMLKMCV"
     gene            complement(1434..1506)
                     /locus_tag="PLQ29_07400"
     tRNA            complement(1434..1506)
                     /locus_tag="PLQ29_07400"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1471..1473),aa:Ala,seq:ggc)
     gene            1621..2781
                     /locus_tag="PLQ29_07405"
     CDS             1621..2781
                     /locus_tag="PLQ29_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPG86512.1"
                     /translation="MSSIVRLSASCAVGIEKVVAGELERLGLAASGRSAGRVGFEADK
                     AGLAKALVGLRCADRVFLEAGRFRARDFDELYEGFKAIPWERWVGPQDRLVIEKARSI
                     RSALSAQNAIQSVGQKAAYERLCGEYGVGRMPETAATVQTRVRIERDEATVELDLCGE
                     ALSRRGYRKRPTEAPLKESVAAAVLFLSGWRRSFPLYDPFCGSGTIPIEAALYAFDIA
                     PGLGRGFSWEAMPDGGAGEVASAKETARAAARLDREVLIAGSDSDQRALDAALANAGM
                     AGVGQRIRFFRAKAEEAAPFAERGFVVTDPPYGKRLGTPAEATALYASLGAFSERFSR
                     WELCFVVDREEFGGFAGERAVPGAAGAWKKTKLVDGAETRWLQRSIASDRRA"
     gene            complement(2756..>3470)
                     /locus_tag="PLQ29_07410"
     CDS             complement(2756..>3470)
                     /locus_tag="PLQ29_07410"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HPG86513.1"
                     /translation="GMGRERFRRALAIAGFPAAIAAVALAAFAFREELTGLFRSSEAI
                     RDAVAAAGSWAPLAFVGLQAIQVIVFFIPGEVVQIAGGFAFGLWGGTLWSVAGILLGS
                     LVNFGVGRLLGRPFAAAALRPERLARIEASTAGGKAVAGFLLLFAIPGIPKDALTYAA
                     GASGLSFRAFIVVSTMGRLPGILGSAYMGSAVFDREYTAALVVLSVALVALFLAFAFR
                     ERLHALVDRLIARLSGPRR"
BASE COUNT          584 a         1128 c         1154 g          604 t
ORIGIN      
        1 gacgcacatc ttgagcatct gcctggcggt atcgaactcc tgcgacaggg cctgcagatt
       61 ggccttcgtg tcctcgccga gggctcctgc cttgtcccgg atcgtctgta cgtccttacc
      121 ggcttcctcg aaggcctcgg cgctcgagac gagctccttg tcggcggcga gcatgcgccg
      181 catgatctcg ccgagctcgg ctatggtctt cttctgagcg tcggaatacg gcgcgggtac
      241 ctggtagtaa cccggtaggc cggcatgttc gcagatgatg tctatgcggt cgcggagcat
      301 cccctcgagc gacttccgct cctcggcctt ggacttgagc agggcctcgg ctgtgtcgcg
      361 cttgcccatc gccttctgga gcaacgtatt gatcctagcc ctctcgtcgc ccctggcctg
      421 gctcacctcg tcggtactgc tcgggttgac ctggcccatc gcgatggagc gcagccactc
      481 gtcgagataa tagaccggct cggaggtcgt cttctgatgg atgatcctgg aaaagatggc
      541 ccgttgctcc gcggatatca tcgtaggcaa cgagaggccg aaacggatca tgagctgctt
      601 tgggacgatg gggttagctg atacgaccct cggtatcata cgcgagacca tattccagta
      661 ggcggtgatc acctgctggc gatacatgcc tcgatccttc ggatcggtgg cctgcaggta
      721 tttcttgagt agctcgtgga agcgctgcga cgcctcgccc tcgccggcga gcgccttctg
      781 gtaatactgg gggtcgtcga agaaggcgtt tggcggatcc gactcgaagc gctcgaccgc
      841 gtcgtaggcc ttcttggaca agtcgacgtc gaattcaccc ttggcttcca cgtcgccggc
      901 gacgcccatg tccccgaaca gcgcctcgaa gcttggcccg ccggccccgg gcactcgtcc
      961 cgccggcttg gccgagtatt cctctattcc gcctccgatg agcgccgcaa tctcaggatc
     1021 gatctcttca gacatcgcgt gccgtccccg cattatagat ggatgctacg tactcgcgta
     1081 gtgtactcca tcgacgcgcc gcggacaagg ttaggggtct gatccccccg cgagtactct
     1141 cgcgcccctc catacgggtt agtaaaagaa aaagaccgcc ggtaggcggc ataacagagg
     1201 gattggtcct aactcgccgg ggtaccggcg agggaattgc cctccattcg aaaacatcct
     1261 gttttctcat tccgggctgc ggttcgcccc ttacgcgcgc atcctgcgcg ctcatcgccg
     1321 cggccgcggc gcggggcgaa ccgttcaatt cccccgcgag tactctcgcg cccctccata
     1381 cgggttagta aaagaaaaga accgccggta ggcggtcctt ttcttttact acgtggaggt
     1441 gaggggaatt gaacccctga cctcttgaat gccattcaag cgctctacca actgagctac
     1501 acccccgaag cgagtggcat tctagcgatc gggccgtagc gtgtcaagcg gatcgcattc
     1561 tttttttggg gcttctgatg tctattgcgt cggccggccc gtgcgacgca tactggttct
     1621 atgagtagca tagtgagact atcggcgtca tgcgccgtgg gcatagagaa ggtcgtggcc
     1681 ggcgagctcg agcggctcgg gctggcggct tcgggcagat cggcgggccg cgtcggcttc
     1741 gaggctgaca aggcgggcct ggccaaggcc ctggtcgggc tacggtgcgc cgacagggtg
     1801 ttcctggagg cgggacggtt ccgagcccgc gacttcgacg agctgtacga aggcttcaag
     1861 gccattccct gggagcgatg ggttgggccg caggacaggc tggtgatcga gaaagcgcgg
     1921 tcgatacgct cggcgctatc cgcccagaac gccatccagt cagtcggcca gaaggccgcc
     1981 tacgagaggc tatgcggcga gtacggcgtg ggccgtatgc ccgagaccgc cgccacggtg
     2041 cagacgaggg tcaggataga gcgcgacgag gcgaccgtgg agctcgacct ctgcggcgag
     2101 gcgctatcgc gacgcggcta ccgcaagcgg ccgaccgagg cgccgctcaa ggagagcgtg
     2161 gccgccgccg tgctgttcct gtcgggctgg cgccgctcct tccctctata cgaccccttc
     2221 tgcggctcgg gaaccatccc gatcgaggca gccctctacg ccttcgatat agccccgggt
     2281 ctcggcaggg gcttctcgtg ggaagccatg cccgacggcg gcgccggcga ggtggctagc
     2341 gccaaggaga cggccagggc cgccgcccgc ctggatcgcg aggtgttgat agccggctcc
     2401 gactcggacc aacgggccct cgacgcggcc ctggcgaacg ccggcatggc cggggtcggc
     2461 cagcgcatac gctttttccg agccaaggcc gaggaggcag cgccgttcgc cgagcggggc
     2521 ttcgtcgtca ccgacccgcc ctacggcaag cgcctcggca cgccagccga ggctaccgct
     2581 ctgtacgcgt cgctcggcgc gttctcggag cgcttctcgc gctgggagct gtgcttcgtg
     2641 gtcgatcggg aggagttcgg cggcttcgcc ggggagcgcg cggtgcccgg cgctgccggc
     2701 gcctggaaga agaccaagct ggtggacggc gccgagacgc gctggctgca gcgctctatc
     2761 gcctcggacc ggagagccta gcgatcaggc ggtcgaccag ggcgtgtagt cgctccctga
     2821 aggcgaaagc taggaacagc gcgaccaggg cgaccgacag gacgacgagc gcggcggtgt
     2881 actcgcggtc gaagaccgcc gaacccatgt aggcgctgcc gagtatgccc ggcaggcgtc
     2941 ccatggtcga cacgacgatg aaggcccgga acgacagccc gctcgcgccg gctgcgtagg
     3001 tcagcgcgtc cttgggaata ccgggtatgg cgaaaaggag caggaagccg gcgacggcct
     3061 ttcccccggc ggtgctggcc tcgatccgcg cgaggcgctc cggcctgagc gccgccgccg
     3121 cgaagggccg gccaagtagc cgcccgacgc cgaagttgac gagcgagccg agcaggatgc
     3181 cggcgaccga ccagagggta cccccccata ggccgaaggc gaagcccccg gctatctgca
     3241 ccacctcgcc ggggatgaaa aatacgatca cctggatcgc ctgaagcccg acgaaggcga
     3301 gcggagccca ggatccggcg gcggcgaccg cgtcgcgtat cgcctcggac gacctgaaca
     3361 gaccggtcag ctcctcacgg aacgcgaagg ccgcgagcgc gacggccgcg atggccgccg
     3421 gaaagccggc tatcgccagc gcgcgacgaa agcgttcccg tcccatgcct
//
LOCUS       DAOPHV010000248         3470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_37421_length_3470_cov_3.667350, whole genome shotgun sequence.
ACCESSION   DAOPHV010000248 DAOPHV010000000
VERSION     DAOPHV010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37421_length_3470_cov_3.667350"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLQ29_07415"
     CDS             <1..254
                     /locus_tag="PLQ29_07415"
                     /inference="COORDINATES: protein motif:HMM:NF036482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86514.1"
                     /translation="GLFVVISAEAPAFYVALEADGLPGRFSDAGFHLAAGETWRLRFL
                     PGAEATSDEAPGAVAPDQPVTADRLSASLRVMDLRSSYE"
     gene            complement(263..1075)
                     /gene="zupT"
                     /locus_tag="PLQ29_07420"
     CDS             complement(263..1075)
                     /gene="zupT"
                     /locus_tag="PLQ29_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HPG86515.1"
                     /translation="MTMNELSALGLTVFAGLSTGVGSLMALMSRKFNPKFLAASLGFS
                     AGVMIYVSMIEIFVKAKTALTGWYGLKPGNVVTVAAFFGGIAVIAVIDKLIPSYENPH
                     EIHNVAACGDEKAFCVDEQKLRRMGLFSALAIAIHNFPEGLATFMSAISDPALGVSIA
                     VAIAIHNIPEGIAVSAPLYYATKSRRTSFWLSFLSGLSEPVGAIIGYFILKPYFNDGT
                     FGIIFAMVAGIMVYISLDELLPTAEEYGEHHIAIGGLIAGMAVMAGSLLLFI"
     gene            1322..2239
                     /locus_tag="PLQ29_07425"
     CDS             1322..2239
                     /locus_tag="PLQ29_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5692 family protein"
                     /protein_id="HPG86516.1"
                     /translation="MFLYQSIPWYSLLMWLAVFAGLVLLNELGRSSKWAGLALFVVLP
                     LALTVTLWPKSAGPGTSMDDWFHRAKVYSALAGCIGFMAIRFIPKVAKHRYALAFPAL
                     ILAVNILEAVIRDFECRSFNGVVGGILTIGGPWNVMNGIAGILNIVTITGWMGIFAGK
                     GKSRDMLWPDMLWFWIIAYDLWNFAYTYNCLGDHSFYCGLALLLSCTFAAFFIKKGAW
                     LQHRAQTLAFWCMFAMSAPWFIDDSIFAVKASGNPKALFLVSLVALVANVAVFAYWLY
                     RILKTRRNPLKAELFTDLKAYKGVAAERA"
     gene            2352..2975
                     /locus_tag="PLQ29_07430"
     CDS             2352..2975
                     /locus_tag="PLQ29_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86517.1"
                     /translation="MNHPELTDQALAILRNPATFQWYFIPILAFVAYVYFNEIQKKNW
                     RGVASGLALYAVHWFYEIVNALIQHFSGHALWTVPTGTSFLLLVGVGWELSMMFAVAG
                     LIFSKILPDDPKAKVLGVNNRVFFAVANAAFFSVFEIFLASTPAFHWVYPWWGAFPVF
                     ITTYVPFFLAANLAYDWKPATRRRLIGGLFIVDAAMLAVFAGVLGWI"
     gene            3036..>3470
                     /locus_tag="PLQ29_07435"
     CDS             3036..>3470
                     /locus_tag="PLQ29_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86518.1"
                     /translation="MPALEAIIAAVILVRYAIGRPDAIGPALAVFLAFQTLVAAGPWP
                     RPGTARQAAPRSAVWLVLAAAKLALCATAYRAEPACVLAWPAVIMGAPLRGPLAAPAD
                     DGWRPVPWVVAAALAAAPSPLIEVGLTPVYLGVWAVACAWAAD"
BASE COUNT          572 a         1175 c         1045 g          678 t
ORIGIN      
        1 agggcctctt cgtcgtcatt tccgccgagg ccccggcctt ctacgtagcg ctcgaggccg
       61 acggcctccc gggcaggttc tccgacgccg gcttccatct cgcggccgga gagacgtggc
      121 gcctgcgctt cctgccgggc gccgaagcca cgagcgacga agccccgggc gccgtcgccc
      181 cggaccaacc cgtgacggct gaccggctaa gcgcctcgct gcgggtcatg gacctgcgtt
      241 cgagctacga gtaggcggaa gcctagatga acaggagcag gctacccgcc atcacggcca
      301 tgcccgcgat gaggccgcct atggctatgt gatgctcacc gtactcctcg gcggtcggta
      361 gcagttcgtc gagcgatatg tagaccatga tgccggccac catcgcgaag atgatgccga
      421 aggtgccgtc gttgaagtac ggcttgagga tgaaataacc gataatcgcg cccacgggct
      481 ccgacaggcc ggacaggaac gacagccaga acgacgtgcg tcggctcttc gtcgcgtagt
      541 acagcggcgc cgagacggct atgccttcgg ggatgttgtg gatcgctatg gccacggcga
      601 tggagacgcc gagcgctggg tcgctgatcg cgctcatgaa cgtcgccagc ccctcgggaa
      661 agttgtgtat ggcgatcgct agcgccgaga atagtcccat gcgcctcagc ttctgctcgt
      721 cgacgcagaa ggccttctcg tcgccgcagg ccgccacgtt gtggatctcg tgcgggttct
      781 cgtacgatgg tatcagctta tcgatgacgg cgatcacggc gatgccgccg aagaacgccg
      841 cgacggtgac gacgttgcct ggcttcaggc cataccaccc ggtcagggcg gtcttggcct
      901 tcacgaaaat ctctatcatc gaaacgtaga tcatgacgcc ggccgagaag ccgagcgacg
      961 ccgcgaggaa tttagggttg aattttctgg acatcaaagc catcaggctg ccgacgccgg
     1021 tcgacaggcc ggcgaagacg gtcaagccca aggcggacag ctcgttcatg gtcataggat
     1081 cgtctccttg gctattacta tacctaggcg cgagccgaac ggctatcgcc gcgcccccgt
     1141 atccctccgg tccgagagtt gcacgccatt atcctataca tgaataatga ctatattgtc
     1201 tatataaacc ttattgtcga taaacttgac actatattac aatatctgta caactatcgc
     1261 gatcacggcg tcgccgcggc tcaacgatac gacgcgcctc cgcgcgttcg aggatgtatc
     1321 catgttccta taccaatcga tcccatggta ttcgctgctc atgtggctgg cggtcttcgc
     1381 cggcctcgtc ctgctcaacg agctcggccg atcgagcaag tgggccggcc tggccctctt
     1441 cgtcgtcctt cctctggcgc tcaccgttac cctctggccg aaatccgccg gcccgggcac
     1501 gagcatggac gattggttcc acagggccaa ggtctactcg gcgctcgccg gctgcatcgg
     1561 cttcatggcc atacgcttca tacccaaggt ggccaagcac cgctacgccc tggcctttcc
     1621 ggcgctgata ctagccgtca acatcctcga ggcggtcata cgcgatttcg agtgccgctc
     1681 gttcaacggc gtcgtcggcg gcatcctgac cataggcggc ccctggaacg tcatgaacgg
     1741 catcgccggt atcctgaaca tcgtcaccat caccggctgg atgggcatct tcgccggcaa
     1801 gggcaagtcg cgagacatgc tctggccgga catgctctgg ttctggatca tcgcctacga
     1861 cctgtggaac ttcgcctaca cctataactg cctcggcgac cactcgttct actgcgggct
     1921 ggccctgctc ctgtcctgca ccttcgcggc cttcttcatc aagaagggcg cctggctcca
     1981 gcaccgcgcc cagaccctcg cgttctggtg catgttcgcg atgagcgcgc cgtggttcat
     2041 agacgactcg atcttcgcgg tcaaggcctc ggggaacccg aaggcgctgt tcctggtcag
     2101 cctcgtggcg ctcgtagcca acgtcgcggt cttcgcctac tggctgtaca ggatcctgaa
     2161 gacgaggcgc aaccccctca aggccgagct tttcaccgac ctcaaggcct acaagggcgt
     2221 ggcggccgag cgcgcctgac gagaggcgag gaatcgctcg cggcggccgg ggcccggccg
     2281 ccgccgttac gctatggagt gcctgcttgg ccgaaccgtg ctatcattgg cggcgtcgat
     2341 aaggaggcac catgaaccat cccgagctga cggaccaggc cctggccatc ctgcgcaacc
     2401 cagcaacctt ccagtggtat ttcatcccca tcctcgcctt cgtcgcgtac gtctacttca
     2461 acgagatcca gaagaaaaac tggcgtggag tggcctcggg actagcgctg tacgcggtcc
     2521 attggttcta cgagatcgtc aacgccctca tccagcactt ctcgggccac gcgctctgga
     2581 cggtaccgac tggcacctcg ttcctgctgc tcgtcggagt cggctgggag ctgtcgatga
     2641 tgttcgccgt cgcgggcctg atcttctcga agatccttcc cgacgacccg aaggccaagg
     2701 tgctcggcgt caacaaccgc gtgttcttcg ccgtggcgaa cgcggccttc ttctcggtct
     2761 tcgaaatctt cctcgcctcc accccggcct ttcactgggt ctacccctgg tggggagcct
     2821 tcccggtgtt catcaccacc tacgtgccct tcttcctcgc tgccaatctc gcctacgact
     2881 ggaagcccgc gaccaggcgg cggctgatag gcgggctgtt catcgtcgac gcggcgatgc
     2941 tcgccgtatt cgccggcgta ctcggatgga tttagccgaa gggtcgacta tagggccgct
     3001 accgacgagg atccgccgcg cccacggagg ctgacgtgcc ggccctggag gctatcatcg
     3061 cggcggtcat tctcgtccgg tacgctatcg gccgccccga cgcgatcggg cccgccctcg
     3121 ccgtcttcct ggcgttccag acgctggtag ccgccggacc gtggccgagg cccggtacgg
     3181 cgcgccaggc agccccgcgc tccgccgtct ggctcgtcct ggcggccgcc aagctggctc
     3241 tatgcgctac cgcctatcgc gccgagccgg cttgcgtcct ggcctggcca gccgtcatca
     3301 tgggcgcgcc gcttcgcggt ccgctagccg cgccggctga cgacggttgg cgccccgttc
     3361 catgggtcgt agccgcggcg ctcgccgcgg cgccgtcccc gctgatcgag gtgggcctga
     3421 cgccggtcta cctcggcgtc tgggctgtcg cctgcgcttg ggccgcggac
//
LOCUS       DAOPHV010000249         3466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_37475_length_3466_cov_3.478745, whole genome shotgun sequence.
ACCESSION   DAOPHV010000249 DAOPHV010000000
VERSION     DAOPHV010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37475_length_3466_cov_3.478745"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..986)
                     /locus_tag="PLQ29_07440"
     CDS             complement(261..986)
                     /locus_tag="PLQ29_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPG86519.1"
                     /translation="MLGSRWSPYTKFFDVMFRSRALALRGLYDDEAWAASSMAIMRAM
                     ERCGATIHISGLDHVRALDGPAVFVANHMSTLEPLLLPGLICPYRPCTYVVKEKLLHG
                     LIWGPIMRSRDPIAVSRVNPRQDLETVLSQGSEHLAAGRSIIVFPQGTRCDVFRREDF
                     NSIGVRLASKAGVPVVPIAVKTDYWGNSRILRGFGPIRRGRPIMIEFARAMTVSGRAK
                     AEHEAIVDFIEGRLRSWGAAIGD"
     gene            complement(1075..>1727)
                     /locus_tag="PLQ29_07445"
     CDS             complement(1075..>1727)
                     /locus_tag="PLQ29_07445"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPG86520.1"
                     /translation="EYSRSSEYSRSSEYSRSSASGRSSANGRSSANERATVWALAAFP
                     VERTGFNGLVHASVQRGEPGLLWADDATVAEIERSLAAMKDDGRLLVVSVHAGSEYVY
                     GPNALQTRLYRSLVDAGADLVLGHHPHVLQPMEWYEGGSHAGLIVYSLGNFVFDDVED
                     NPKGLESMLLSLGVTDGRIRTLRVYPARMDGYTVDLDDGDAIRSLLWTMSEAWAEE"
     assembly_gap    1728..1827
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1828..2840)
                     /locus_tag="PLQ29_07450"
     CDS             complement(<1828..2840)
                     /locus_tag="PLQ29_07450"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPG86521.1"
                     /translation="MPHADDAARPRFPRLAAAQIVASAAAIVFLIPSSCARAPRYAID
                     SSIDDSAVASTLSAYPVPGHWRRVDPRKADIMVSVESSWGVFEDVVPGARTVSAEAYA
                     VAGDWLDPRESLRSAETGLVAAAALAEPLSSITMPRKALAYDGLYPGDSGYPLVKSLR
                     VSAVPIGRDGRGVFAAEASAYLDALPEAAPPTGLTWIGAVGDVMPGLGTSAMLLELDG
                     PERVFKRLLPVMRSQDLLIANLETAVTQRGVEWPKTFRFRMPAAALTPLMDSGVDVVG
                     LANNHVYDYTDIGFSDTVAALKAAGLPFIGAGLDWNEALRPFDWSSEYSRSSEYSRSS
                     EYSR"
     gene            complement(2872..>3466)
                     /locus_tag="PLQ29_07455"
     CDS             complement(2872..>3466)
                     /locus_tag="PLQ29_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPG86522.1"
                     /translation="AAGPALPGAEIRVADERPADDDPSRLVGELQVRGPMVGPGYYLD
                     PVRTAESLTPDGYFRTGDLGYRDERGRFFVRGRSKTMILGASGENIYPEEIEAELSRC
                     PHVEEALVYGGDEGLVALVHLRPEAISEALAKVKAGVDQAERVVGELLESIKRETNQR
                     LASFCRIRTVEHHPDSFQKTPTQKIKRFLYPDRASSR"
BASE COUNT          594 a         1104 c         1120 g          548 t
ORIGIN      
        1 ccagccgccc gacagggacc gcccgacggg cggggaggtc tgtatgacta tggtcgatag
       61 ccctcgctcg acgagagcgg agctacagga gagcgaggcg gccgacgccg ctaggatcgc
      121 gatcgatagc gtgatcgtca ttgattttct catacggact cctctggaac gattggcgat
      181 gcatctacgt tggtgatcgt ctgaggggta ttgatcgagc atctactcga ttatagcacg
      241 gcgcggccgc tacgaccggc ctaatcgcca attgccgcgc cccaggagcg caggcgcccc
      301 tcgatgaagt cgacgatggc ctcgtgctcg gctttggcgc ggcccgacac ggtcatggcc
      361 cgagcgaact cgatcatgat cggccgcccg cggcggatcg gcccgaaacc gcgcaagata
      421 cgcgagttgc cccagtagtc ggtcttgacc gcaatcggca cgaccggtac gccggccttc
      481 gacgccagtc tgacgccgat gctgttgaag tcctcccgcc tgaacacgtc gcaacgggtg
      541 ccctggggga acacgatgat cgaacgtccg gccgccaagt gctcgctacc ctggctcagc
      601 accgtctcga ggtcctggcg cggattgacg cggctcacgg ctatcgggtc gcgcgagcgc
      661 atgataggcc cccagatgag cccgtggagc agcttctcct tgaccacgta ggtacagggc
      721 ctatacgggc agatgagtcc gggcaagaga agcggctcga gggtgctcat gtggttcgcc
      781 acgaagaccg ccggcccatc gagcgcccgt acgtggtcga ggccgcttat atggatggtg
      841 gctccacagc gttccatagc gcgcatgata gccatcgagc tggcggccca ggcctcgtcg
      901 tcatagaggc cccgtagggc cagggccctg gaacggaaca tcacgtcgaa gaacttggta
      961 tacgggctcc agcgcgaacc gagcatcagg tactcgcgaa ggcatcgccg cgtgcctgcc
     1021 ggcgtgtcat acgcatcgcc atgtatgatt tcgttaccat accctgccat cgtatcactc
     1081 ctcagcccag gcttcgctca tcgtccagag tagcgagcgt atggcatcgc cgtcgtcgag
     1141 gtcgaccgtg taaccatcca tccgcgccgg gtagacccgg agggtcctga tccttccatc
     1201 cgtcacgccg agcgacagga gcatggactc gaggcccttg ggattatcct ccacgtcatc
     1261 gaagacgaaa ttgccgagcg agtagacgat caggccggcg tgggagccgc cttcgtacca
     1321 ctccatcggc tggagcacgt gaggatggtg gcctagcacc aggtcggccc cggcgtcgac
     1381 gagcgatcgg tacagcctcg tctgcagggc gttcgggccg tagacgtact cgctgcccgc
     1441 gtgcaccgac acgacgagga ggcggccgtc atccttcatc gcggcgaggc tccgctcgat
     1501 ctcggcgacc gtcgcgtcgt cggcccagag caggcccggc tcgccgcgct ggaccgaggc
     1561 gtggacgagg ccgttgaagc cggtgcgctc taccgggaat gcggcgagcg cccagaccgt
     1621 tgcccgctcg ttcgcggacg accggccgtt cgcggacgac cggccgctcg cggacgaccg
     1681 gctgtactca gacgaccggc tgtactcaga cgaccggctg tactcagnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnncgg ctgtactcag acgaccggct gtactcagac
     1861 gaccggctgt actcagacga ccagtcaaag ggcctcagag cctcattcca gtccagaccg
     1921 gcgccgatga acggcaggcc ggcagccttg agcgcagcca cagtatcgga gaagccaatg
     1981 tcggtgtagt cgtagacatg gttattagcc aggccgacga catctacgcc gctgtccatg
     2041 aggggcgtaa gcgccgccgc cggcatcctg aaacggaagg tcttgggcca ttcgacgccg
     2101 cgctgggtca cggccgtctc gaggttggcg atcagcaggt cctgcgagcg catcaccggt
     2161 agcagtcgct tgaagaccct ctccggccca tcgagctcga gcagcatggc cgatgtaccc
     2221 aaaccaggca tcacatcgcc aacggcgcct atccaggtca gtccggtggg gggcgcggcc
     2281 tcgggcagag catccaggta ggctgatgcc tcggctgcga atacaccgcg gccgtcgcgc
     2341 ccgatgggca ccgcgctcac tcgcagcgac ttgaccagcg gatagccgga gtcgccgggg
     2401 tagagaccgt cgtaggccag cgccttgcgg ggcatcgtta tcgacgagag gggttcggcc
     2461 agcgcggccg cggcaacaag gccggtttcg gctgaacgga gagactctct cggatccagc
     2521 cagtcgcccg ccacggcgta ggcctcggcg ctcaccgtgc gggcgcccgg cacgacgtcc
     2581 tcgaagacgc cccaggacga ctcgacggac accatgatgt cggccttgcg tggatcgacc
     2641 cgcctccagt ggcctggaac cgggtacgcc gacagcgtag acgcgacggc tgagtcgtca
     2701 atggacgagt cgatcgcgta acgaggcgcc ctggcgcagg aggacgggat gaggaatact
     2761 atcgccgcgg ctgacgcgac gatctgagcg gcggcgaggc gcggaaagcg cggcctggcc
     2821 gcgtcgtcag catggggcat accgcgagga taccagcggt cgccccgccg gtcaacgcga
     2881 ggaggcgcga tccgggtaca agaagcgctt tatcttctgg gtaggcgtct tctggaacga
     2941 gtcgggatgg tgctccaccg tgcgaatacg gcagaaggac gcgagacgct gattcgtctc
     3001 gcgcttgatc gactcgagca gttcgcctac gacgcgctcc gcctggtcga cgccggcctt
     3061 gaccttcgcg agggcctcgc ttatcgcctc gggccgcagg tgcacgagag ccacgagccc
     3121 ctcgtcgccg ccgtagacca gagcctcctc gacgtgtgga cagcggctca gttcggcctc
     3181 gatctcctcg gggtagatgt tttcccccga ggcgccgagt atcatggtct tggagcgtcc
     3241 gcgtacgaag aagcggccgc gctcgtcgcg gtagccaagg tcgccggtgc ggaaatagcc
     3301 gtcgggcgtg aggctctcgg ccgtacgcac ggggtccagg taatagcctg gcccgaccat
     3361 cggtccgcgt acctgcagct ctccgacgag gcggctcggg tcatcgtcgg ccggccgctc
     3421 atcggcgacg cgtatctccg ctccgggcag ggccggtccg gccgcc
//
LOCUS       DAOPHV010000250         3458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_37595_length_3458_cov_4.354687, whole genome shotgun sequence.
ACCESSION   DAOPHV010000250 DAOPHV010000000
VERSION     DAOPHV010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37595_length_3458_cov_4.354687"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLQ29_07460"
     CDS             <1..530
                     /locus_tag="PLQ29_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86523.1"
                     /translation="SYRTPVDIKGGILVTTAEGGRTVADIGQAISFERVQELEAAGSR
                     VLFNCATGLFDLARLVPRLDEIARRLPVRISDQDKDAGRYSQAEQSTWEVVGLIDEPL
                     GFAVEKGERFIAAKLLAETVLASGTAGAGAAGAGAALPPSVAATAETMSRGLSDVLSG
                     PCGLRLDGGAWRPAQ"
     gene            complement(565..2916)
                     /gene="lon"
                     /locus_tag="PLQ29_07465"
     CDS             complement(565..2916)
                     /gene="lon"
                     /locus_tag="PLQ29_07465"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPG86524.1"
                     /translation="MPDNEIIPIDMLLPNKIPIIPLNGKPIFPGIFTPLMLGSQDDIQ
                     TIDDAMQTDGMVGLVLVRDDTDKPKASDLHEVGTVAKIVKRINLPDGSVNIFISTLKR
                     FKIKKALSKEQPIMAAVEYLEDQGEGPDEIKALTRALLSEMKQISENNPLFSEEMRLN
                     MINIDHPGKIADFIASILNIDKVEQQKILELSDIKERMERVLMSIKREQELLKIQKRI
                     QSEINEKVEKSQRDYFLREELKAIKQELGMPADAKGTDFQRFKERLDAFGFSGEVKEL
                     VDQELEKFSLMDPNSSEFIVTRNWLDLVSNLPWKAETGKDFDIHAARDILEGDHYGLK
                     DVKDRIIEYLAVRKLKRDAKGSILCLVGPPGVGKTSVGRSIARALGKEFFRFSVGGMR
                     DEAEIKGHRRTYVGALPGKIIQGLKITKSRDPVFMIDEIDKMGSSYQGDPSSALLEVL
                     DPEQNVAFRDHYLDLPFDVSNVFFIVTANTLDTIPGPLRDRMEIIQLSGYVDSEKLEI
                     AKRYLIPKSLEKNGLKKARVKYTRDALTAIAEGYAREAGVRNFEKYLDRIHRKIALAV
                     VTAPEGEDTKRFEVDKPSLEKYLGKPRFDADEVKRATLPGMTVGLAWTSMGGDTLIIE
                     AVANPGKEGFKLTGQMGSVMQESAGIAYTWVRHHVADAHGIDAKFFEERQIHLHIPEG
                     ATPKDGPSAGITMATSLASLVLGKRVKDRLAMTGELSLTGQVLPIGGLREKTVAAKRN
                     KIREIIIPRANEKDLSDIPEHVKKGIVFHPVDRMEEVLEIALS"
     gene            complement(3053..>3458)
                     /locus_tag="PLQ29_07470"
     CDS             complement(3053..>3458)
                     /locus_tag="PLQ29_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPG86525.1"
                     /translation="VKHRDLFLELIRSVCSWTFMNEDEFIALSQTGVDEGLKEFSASA
                     PGTVIVKRAETGAVCVSDGHLLESAVRPIRATDTTGAGDAFAAGFLSAALRGEPLARC
                     LRLGNRVAEQAIQVTGMALDADRLQRAASTVL"
BASE COUNT          566 a         1057 c         1096 g          739 t
ORIGIN      
        1 tttcgtaccg gaccccggtc gacatcaagg gaggcatcct cgtcacgacg gccgaaggcg
       61 ggcgcacggt agccgatatc ggccaagcca tctccttcga gcgcgtccag gaactggagg
      121 ccgccggctc gagggtgctg ttcaactgcg ccaccggcct gttcgaccta gcgcgcctcg
      181 tgccgaggct cgacgagatc gccaggcgcc tgccggtgcg tatctccgat caggacaagg
      241 acgccggacg ctatagccag gccgagcagt cgacctggga agtcgtcgga ctgatcgacg
      301 agccgctcgg cttcgccgtg gagaaaggcg agcgcttcat cgccgccaag ctcctggccg
      361 agacggtcct ggccagcggc accgcgggag cgggagccgc cggagcggga gccgccttgc
      421 cgccgagcgt cgcggcgacc gctgaaacga tgtccagggg gttgagcgac gtgttatctg
      481 ggccgtgcgg gcttaggctc gatggcggag cctggcgtcc cgcccagtaa aacggcggcc
      541 ggccccgagg ggccggccgt tccgctacga taaggctatc tcgagcactt cttccatccg
      601 gtccaccgga tggaaaacga tgcctttctt gacgtgctcc gggatatccg acaggtcctt
      661 ctcgttggcc ctcgggatga tgatctccct gatcttgttg cgcttcgcgg cgacggtctt
      721 ttccctgagg ccgccaatcg gcagcacctg gccggtcagg gacaactctc cggtcatagc
      781 cagcctatcc ttgacccgct tgccgagcac gagcgacgcg agcgaggtgg ccatggtgat
      841 gcctgcgctc ggtccgtcct tgggcgtggc gccctccggg atatgcagat ggatctggcg
      901 ctcctcgaag aacttcgcgt cgatgccgtg ggcgtccgcc acgtggtgcc tgacccaggt
      961 ataggcgatg ccggcggatt cctgcatcac cgaacccatc tgtccggtca gcttgaagcc
     1021 ttccttgccg gggttggcca ccgcctcgat gatcagcgta tcaccgccca tgctggtcca
     1081 ggctaggccg acggtcatgc ccggcagcgt cgcgcgcttg acctcgtcgg cgtcgaagcg
     1141 cggcttgccc aggtacttct cgagtgacgg cttgtccacc tcgaagcgct tcgtatcctc
     1201 gccttcgggc gccgttacga cggccagcgc tatcttgcga tggatgcgat ccaggtactt
     1261 ctcgaagttc cggacgccgg cctctctcgc gtagccctcg gcgatggcgg tcaacgcgtc
     1321 gcgggtgtac ttgacgcggg ccttcttgag gccgttcttc tcgaggctct tcgggatcag
     1381 gtagcgcttg gcgatctcga gcttctccga gtcgacgtag ccggatagct ggatgatctc
     1441 catgcggtcg cgtagcggcc cggggatggt gtcgagggta ttggccgtga cgatgaagaa
     1501 cacgttggag acgtcgaagg gtaggtcgag gtaatgatcc cggaacgcca cgttctgctc
     1561 cgggtcgagc acctcgagca gggcgctcga cggatcgccc tggtagctcg agcccatctt
     1621 gtcgatctca tcgatcatga agaccgggtc gcggctcttg gttatcttga gcccttggat
     1681 gatcttgccg ggtagcgccc cgacataggt gcgccgatgg cccttgattt ccgcctcgtc
     1741 gcgcatgccg ccgaccgaga aacggaagaa ctccttgccg agagcccggg cgatggatct
     1801 accgacgctg gtcttgccga cgccgggcgg gcctaccagg cacaggatag agcccttagc
     1861 gtcgcgtttg agcttacgga ctgccagata ctcgatgatg cggtccttga cgtccttgag
     1921 gccgtagtgg tcgccctcga ggatgtcgcg ggcggcatgt atgtcgaagt ccttgccggt
     1981 ctccgctttc caaggcaggt tcgagaccag atcgagccaa ttcctcgtca cgatgaactc
     2041 cgacgaattc gggtccatca ggctgaattt ctcgagctcc tggtcgacga gctccttgac
     2101 ctcgcccgag aagccgaacg cgtccagccg ctccttgaag cgctggaagt ccgtgccctt
     2161 cgcgtcggcg ggcataccga gctcttgctt gatggccttg agctcctccc gcagaaaata
     2221 gtcgcgctgg ctcttctcga ccttctcgtt gatctcgctc tggatgcgtt tctggatctt
     2281 gaggagctcc tgctcgcgct tgatcgacat gaggacgcgt tccatacgct ccttgatatc
     2341 gctgagctcg agaatcttct gctgctcgac cttgtctata ttcaggatgc tggcgatgaa
     2401 gtcggctatc ttgcctggat ggtcgatgtt gatcatgttg aggcgcatct cctcggagaa
     2461 cagtggattg ttctccgaga tctgcttcat ctccgacagc agcgccctgg tcagcgcctt
     2521 gatctcgtcc gggccttccc cctggtcctc gaggtactcg accgccgcca tgatgggctg
     2581 ttccttggac agggccttct tgatcttgaa gcgcttgagg gttgagatga atatattgac
     2641 cgagccgtcg ggaaggttga tccgcttgac gatcttggct accgtaccca cctcgtggag
     2701 gtcgctggcc ttgggcttgt cggtatcgtc ccggacgagc acgagcccga ccatgccgtc
     2761 ggtctgcata gcatcgtcga tggtctggat gtcgtcctga ctcccgagca tcaagggcgt
     2821 aaagatgccg gggaatatag gctttccatt caacggaatg attggaattt tattcggtag
     2881 tagcatgtcg atcggtatta tctcgttgtc gggcattctc cgccgtcctt tcgcttagtt
     2941 tcgctataaa atatagaggt tttggcccga caggcaaggc ttttgactag ctacggaagc
     3001 gttttggcat aggaacggag acttgtggcc tagttggacg atacgaccag tcttatagca
     3061 cggtggaggc ggctcgctgg agccgatcag cgtcgagcgc catacccgtc acctggatcg
     3121 cctgctccgc gacgcggttt ccgagtcgga ggcaacgcgc gagcggctcg cctcggaggg
     3181 ccgccgacag gaagccggcg gcgaacgcgt ctccggctcc ggtcgtgtcg gtggcgcgga
     3241 tcggccgaac cgcgctctcg agcaggtgcc cgtcgctgac gcagacggcg cctgtctcgg
     3301 ctcgcttgac tatgacggtg ccaggcgctg acgcggagaa ttccttgagg ccctcgtcga
     3361 ccccagtctg gctcagcgcg atgaattcgt cctcgttcat gaaggtccag gagcagaccg
     3421 agcggataag ctctaggaac aggtcgcgat gcttgacg
//
LOCUS       DAOPHV010000251         3451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_37711_length_3451_cov_3.558893, whole genome shotgun sequence.
ACCESSION   DAOPHV010000251 DAOPHV010000000
VERSION     DAOPHV010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37711_length_3451_cov_3.558893"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2110)
                     /locus_tag="PLQ29_07475"
     CDS             complement(<1..2110)
                     /locus_tag="PLQ29_07475"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPG86526.1"
                     /translation="MRPIKTILATALAIAFAAAAFAQAPQSLILEFVDGDNLDVVYPD
                     STVYSYATGSVMEGDAIPIGATVKTGADTSVELRLKPNGTVVKLAKMTSFRVDGLATP
                     QKDQNAFTIASGKIRAVAAKGSQYTIYTSTTVAGVRGTDFSMSFEEGAKAMLLVAKGR
                     VEFGRRGEGDAMADAIMVGAGQFADFFKGLTAAPYSAEQFAEEYSGLDIPLERIPEQA
                     EDQPPAEEAKAEEASQATGPTDALASDFSPAELPAADRSDDDATADKAASEAGGKVAE
                     WLRDVLGMEIGSITINGETWAKAVIQPTFAVGKLKTGLYLPVIYSSNLFDPSTWYRPK
                     GNDEWSFGTDVGWTAETWPEALLDAASDLALKIKFIEYGKPLVDPFFVKVGNLDSFTI
                     GHGLLMRDYANDTDFPSIRRVGLNLGLDQGSWGFEAMTNDLTDNQIVGGRLYIRPIPG
                     FKLALGLSGVADLYPASDYGDDAVLYGDPAFIGGALDLDLPIVTSGLLGIRLFADGAA
                     MAPYVRNAFSYGGFDAADGFRYDMVWTGDSIKNWGASAGLLGNVLFVDWRLEYRYYTG
                     AFTPAFFDAGYERRRSSLVSEWAGYLSGAEEIAQSISTMGVYGEAGASILKDKLSFAL
                     GYFWPWSPDASGLAEQLELTEDYLKATLTVKKGLIPVIDLAGSVSYERKNFVGTLAGD
                     ADGVSLFDENTTFSGELSVPV"
     gene            complement(2130..>3451)
                     /locus_tag="PLQ29_07480"
     CDS             complement(2130..>3451)
                     /locus_tag="PLQ29_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86527.1"
                     /translation="VCLIVVISMPLAFGEDAGGLGFEAGISLGTDVLIDAEGQPETWN
                     SLGFQPDLAIGPFGVGFDLTMRFKLMPSVDTALAIFPGDWVPDYEGSGKTLLDLYLPK
                     IMYVRYGQRGDPLYAKLGSIDDLTLGNGFIVGEYSNTRFLPGQRLFGLDVGVDGALFG
                     FPYVGIELLTGNLARLDVFGGRLFARPLVSTGMPILENLQVGATMAMDRGVSNFASSE
                     TFSPVLAYGADVFLPLLTGQAFPLAAFSELAFEPKGRTGFMVGAGGRLIGVITYAAQL
                     RLLGAGFSPVYFDANYDLFRVQKAALMESDPTGEGMAGWLAKAGASLFEDKLYFSVSV
                     DGPFKATPAVASDSSAEYPHLRGVIGMAEGVLGGFFVSGSYDKYFIGRENGFFADLVD
                     PNDAVISAAFNYKTGAAVFTLLYNLSYNPEADKGFDVTSSLQSSIKF"
BASE COUNT          593 a         1079 c         1183 g          596 t
ORIGIN      
        1 gtacgggcac cgacagttcg ccggagaacg tggtgttctc gtcgaatagc gatacgccat
       61 ccgcgtcgcc ggccagggtg cccacgaagt tcttgcgctc gtaggatacg gagccggcca
      121 ggtcgatgac cggaatcagg cccttcttga ccgtaagcgt cgccttgagg tagtcctcgg
      181 tcagctcgag ctgctcggcc aggccggagg cgtccggcga ccagggccag aagtagccga
      241 gggcaaagct cagcttgtcc ttgaggatgc tggctccggc ctcgccgtag acgcccatcg
      301 tcgagatgga ctgggctatt tcctcggcac cggacaggta gcccgcccac tcggatacga
      361 gcgacgaacg cctgcgctca taaccggcgt cgaagaaggc cggcgtgaag gctcccgtgt
      421 agtagcggta ctcgagcctc cagtctacga acagcacatt gcccaggagg ccggccgacg
      481 cgccccagtt cttgatcgag tcgccggtcc agaccatgtc gtagcggaaa ccgtccgcgg
      541 cgtcgaagcc accgtagctg aaggcgttcc tgacgtacgg ggccatcgcg gcgccgtcgg
      601 cgaagagccg tatgccgagg aggccactcg tgacgatggg caggtcgagg tccagggcgc
      661 cgcctatgaa ggccgggtcg ccgtagagga ccgcgtcgtc gccgtagtcg gaagcggggt
      721 agaggtcagc gacgcctgac aggccgaggg ccagcttgaa gccggggatg gggcgaatgt
      781 agagccggcc gccgacgatc tggttgtcgg tcaggtcgtt ggtcatcgcc tcgaagcccc
      841 aggatccctg gtcgaggccg aggttcaggc cgacgcggcg tatcgacggg aagtccgtgt
      901 cgttcgcata gtcgcgcatc aggaggccgt ggcctatcgt gaagctgtcc aggttgccga
      961 ccttgacgaa gaaggggtcg acgagcggct tgccgtactc gatgaatttt atcttgagcg
     1021 ccaggtcgga ggcggcgtcg agcagggcct cgggccaagt ctcggcggtc cagcccacgt
     1081 cggtgccgaa ggaccactcg tcgttgccct tgggccggta ccaggtggac gggtcgaaca
     1141 ggttgctcga gtagatgacc ggcaggtaga ggccggtctt gagcttgccg accgcgaagg
     1201 tcggctggat gacggccttg gcccaggtct cgccgttgat cgtgatcgag cctatctcca
     1261 tgccgagcac gtcgcgcagc cactcggcga ccttgcctcc ggcttcggac gcggccttgt
     1321 cggccgtggc gtcgtcgtcg cttcgatcgg ccgcgggaag ctccgccggc gagaagtccg
     1381 acgcgagcgc gtcggtcggg ccggtcgcct gggaggcctc ctcggccttc gcttcctcgg
     1441 ccggaggctg gtcctcggcc tgctcgggta tacgttcaag cggtatatcg agacccgagt
     1501 actcctcggc gaactgctcc gcagaatacg gggcagccgt aaggcccttg aagaagtcgg
     1561 cgaactggcc cgcgccgacc atgatggcgt ccgccatcgc gtcgccctct ccgcgcctgc
     1621 cgaattcgac cctgcccttg gcgacgagga gcatcgcctt ggcgccctcc tcgaacgaca
     1681 tggagaaatc ggtgcctcgg acgccggcga cagtggtcga ggtgtagatc gtgtactgcg
     1741 agcctttggc cgctaccgcc ctgatcttgc cggaggctat cgtgaaggcg ttctggtcct
     1801 tctggggcgt cgccagaccg tcgaccctga agctcgtcat cttggccagc ttgacgacgg
     1861 tgccgttcgg cttgagccgt agctcgacgc tcgtgtcggc gccggtcttg accgtcgcgc
     1921 cgatcgggat ggcgtcgccc tccatcaccg atcccgtcgc atagctatag accgtcgagt
     1981 ccgggtacac gacgtcgagg ttgtcgccgt cgacgaattc gaggatgagc gattgcggag
     2041 cctgcgcgaa cgcggcggcc gcgaaggcga tggccaaggc cgtcgccagt atcgttttta
     2101 tcgggcgcat atggatcctc cccttgccgt cagaatttga tcgagctctg caggctcgag
     2161 gtcacgtcga agcccttgtc ggcctccggg ttgtagctca ggttatagag cagggtgaac
     2221 acggccgctc cggtcttata gttgaaggcc gccgagatga ccgcgtcgtt cgggtcgacg
     2281 agatcggcga agaagccgtt ctcccgcccg atgaagtact tgtcgtacga gcccgagacg
     2341 aagaagccgc cgagcacgcc ctcggccatg ccgatcacgc cgcgcaggtg cgggtactcg
     2401 gcgctactgt cgctggcgac cgcgggtgtc gccttgaagg ggccgtcgac cgatacgctg
     2461 aagtacagct tgtcctcgaa gaggctggcg ccggccttgg ccagccagcc ggccatgccc
     2521 tcgccggtcg ggtcgctctc catgagcgcg gccttctgga cgcggaacag gtcgtagttg
     2581 gcatcgaagt agaccggcga gaagccggcg cctaagaggc gcaactgggc cgcgtaggtg
     2641 atgacgccga tgaggcggcc accggcgccg accatgaagc cggtccgccc cttcggttcg
     2701 aaggccagct cggagaaagc ggcgagcggg aaggcctgcc cggtcagtag gggcaggaag
     2761 acgtcggcgc cgtaggcgag caccggcgag aaggtctcgc tcgaggcgaa gtttgacacg
     2821 cctcggtcca tcgccatcgt cgcgccgacc tgaaggttct cgaggatcgg catgcccgtc
     2881 gagacgaggg gccgcgcgaa gagacggccg ccgaaaacgt cgagcctggc caggttgccg
     2941 gtcaggagct cgatcccgac gtaggggaag ccgaagagcg ccccgtcgac gccgacgtcc
     3001 agcccgaaaa ggcgctggcc cggtagaaag cgcgtgttcg agtattcgcc gacgatgaag
     3061 ccgttgccga gggtcaggtc gtcgatcgag cccagcttgg catagagcgg gtctcccctc
     3121 tggccgtagc gtacgtacat gatcttgggc aggtacaggt cgagcagggt cttgcccgag
     3181 ccttcgtagt ccggtaccca gtctccgggg aagatggcca gcgcagtgtc aacgctcggc
     3241 atcaacttga agcgcatggt caggtcgaac ccgaccccga acggcccgat agccaggtcg
     3301 ggctggaagc cgaggctgtt ccaggtctcg ggctggcctt cagcgtctat gaggacgtcc
     3361 gtccccaagg atatccccgc ctcgaaaccc agtcccccgg cgtcttcgcc gaaggctaag
     3421 ggcattgata tcactacaat gaggcatact a
//
LOCUS       DAOPHV010000252         3441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_37866_length_3441_cov_4.007088, whole genome shotgun sequence.
ACCESSION   DAOPHV010000252 DAOPHV010000000
VERSION     DAOPHV010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37866_length_3441_cov_4.007088"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLQ29_07485"
     CDS             <1..846
                     /locus_tag="PLQ29_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPG86528.1"
                     /translation="TLAQAAFIVDNGAAEYRRLGTAGSAGTKTYALTGNVANTGLIEV
                     PFGATLRHVVFDIGGGILNEDGKPDNGAFKAVQIGGPSGGCLTAEHLDVPMDFDSLGE
                     IGAMVGSGGLVVMNDSTCMVQMARFFMQFTQRESCGKCVPCREGTQQILALLDDIIAG
                     NGTLETLDLLEELCENVAASSLCGLGASAPAPVLSMLRQFRPEYEAHVVEKRCPTGQC
                     AALSPLRIAPELCKGCTACGKKCPVGAIAGSPKQAHSIDPEKCIKCGACIAACKFDAI
                     KKGAA"
     gene            843..2957
                     /locus_tag="PLQ29_07490"
     CDS             843..2957
                     /locus_tag="PLQ29_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HPG86529.1"
                     /translation="MSGFVTIDGIKVPIEGEPNLLALCRKANIDIPTFCYHSELSTYG
                     ACRLCLVEINGKDIDASCVVPPKDGMSIRTSTARIRSIRRMNLELLLANHKQDCPTCE
                     RSASCKLRDLSAKMGIRDVRFKRLRDERPKDEGSVALVRDPNKCILCGDCVRYCSEIQ
                     GVGAIDFAFRGSDVVVSPAFGKSLGSVDCVNCGQCAAVCPTGAIVPRSPAEAVWQALD
                     DPDAVVVAQVAPAVRVAIGERFGLAAGADATGKLVTALRMLGFGRVYDTAFGADLTVA
                     EEAGELLGRLERGEKLPLFTSCCPAWVKYAEQFFPELLPSLSSCMSPQSMMGSIIRDD
                     EARRASSSAALPSSAAAGALGAGTEAERVKGGVAKRIVVVSIMPCVAKKYEVSRPELA
                     RDGSPLVDYALTTAELASMVEEAGIRFADLRPGAMDLPFGFATGAGVAFGNAGGVAEA
                     VARYVGSPVSPLTGAALEFKDEAPGGGIRAATIIAGGRELTIVSVQGLANAKTVARGV
                     LDGTRKADIIEVMACPGGCVGGAGQPIGFKLGAVAERRLGLADADGGRELRASGSNPF
                     LSRFYADVIGGAPGSQRSHELLHTRYSSKKRIQDTSIPLIRGGGAEKVTVKVCVGTSC
                     FLRGSQAVLRDVLAEVERTGLQDFVDVSATFCSERCDKGPTVHVGGKTIHKADAEAVM
                     AAIRPLLAHPAVAAEAAAVSSR"
     gene            complement(3034..3156)
                     /locus_tag="PLQ29_07495"
     CDS             complement(3034..3156)
                     /locus_tag="PLQ29_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86530.1"
                     /translation="MPRPIWRLRRLAGWLAGPELSASFAYRFMKRPSVPSRPDV"
     assembly_gap    3203..3212
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3213..>3441)
                     /locus_tag="PLQ29_07500"
     CDS             complement(<3213..>3441)
                     /locus_tag="PLQ29_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPG86531.1"
                     /translation="AYAASKAYALSLSVGLAAEWKARGITVCALCPGPVATEFSLVAS
                     AGARKEVKHGWPAVKTARACLRDAISGAWIS"
BASE COUNT          533 a         1184 c         1180 g          534 t
ORIGIN      
        1 acgctggccc aggccgcctt catcgtcgac aacggggcgg ccgagtaccg caggctcggc
       61 acggccgggt ccgcgggtac caagacctac gcgctgaccg gcaacgtggc gaacacgggc
      121 ctgatcgagg tgcccttcgg cgccaccctg cgccacgtcg tcttcgacat cggcggaggc
      181 atcctgaacg aggacggcaa gccggataac ggcgcgttca aggccgtgca gataggcggc
      241 ccgtccggcg gctgcctgac cgccgagcat ctggacgtgc cgatggattt cgactcgctc
      301 ggcgagatag gcgccatggt cggctccggc ggcctcgtcg tcatgaacga ctccacctgc
      361 atggtgcaga tggcgcgctt cttcatgcag ttcacccagc gcgagtcctg cggcaagtgc
      421 gtaccctgcc gcgagggcac ccagcagatc ctggccctgc tcgacgatat catagccggg
      481 aacggcacgc tcgagaccct cgacctgctc gaggagctgt gcgagaacgt cgcggccagc
      541 tcgctctgcg ggctcggcgc ctcggcgccc gcgccggtgc tgtcgatgct caggcagttc
      601 cgccccgagt acgaggcgca cgtcgtcgag aagcgttgcc cgaccggcca gtgcgcggcg
      661 ctgtcgccgc tccgcatagc cccggagctg tgcaaaggct gtaccgcttg cggcaagaag
      721 tgcccggtcg gcgccatcgc cggctcgccg aagcaggcgc actcgataga tccggagaag
      781 tgcatcaagt gcggcgcctg catcgcggcc tgcaaattcg acgctatcaa gaagggggcg
      841 gcatgagcgg cttcgtgacc atagacggca tcaaggtacc catcgagggc gagccgaacc
      901 ttctcgcgct gtgcaggaag gccaacatcg acataccgac gttctgctac catagcgaac
      961 tgtcgacgta cggggcctgc aggctctgcc tggtcgaaat caacggcaag gatatcgacg
     1021 cgagctgcgt cgtaccgccc aaggacggca tgtcgatccg gaccagcacg gccaggatac
     1081 gctccatacg gcgcatgaac ctggaactgc tcctggccaa ccacaagcag gattgcccga
     1141 cctgcgagcg ctccgccagc tgcaagctgc gcgacctgtc ggccaagatg ggcatacgcg
     1201 acgtgcgctt caagcgcctg cgcgacgaga ggcccaagga cgagggttcg gtcgcgctgg
     1261 tgcgcgaccc gaacaagtgc atactctgcg gcgactgcgt gcgctactgc tccgagatcc
     1321 agggcgtcgg ggcgatagac ttcgcgttcc gcggctccga cgtcgtcgtc agccccgcct
     1381 tcggcaagtc cctcggctcg gtggattgcg tcaactgcgg ccagtgcgcc gccgtctgtc
     1441 cgaccggcgc catcgtgccg cgctcgccgg ccgaggccgt ctggcaggcg ctcgacgacc
     1501 ccgacgccgt cgtcgtggcg caggtggctc cggcggtccg cgtggcgata ggcgagcgct
     1561 tcggcctggc ggccggcgcc gacgcgacgg gcaagctggt gaccgcgctg cgcatgctcg
     1621 gtttcggccg cgtctacgac acggccttcg gcgcggatct gacggtcgcc gaggaggccg
     1681 gcgagctgct cggccggctc gagcgcggcg agaagctgcc gctgttcacc tcgtgctgcc
     1741 ccgcctgggt caagtacgcc gagcagttct tcccggagct gttgccgagc ctcagctcgt
     1801 gcatgtctcc tcagtcgatg atgggctcga tcatacggga cgatgaggcg cggcgcgcct
     1861 cgagctcggc ggcgctgccg agctcggcgg ccgccggggc tctgggcgcc ggtacggaag
     1921 ccgaacgcgt taaaggcggc gtagccaagc ggatcgtcgt cgtctcgatc atgccctgcg
     1981 tcgccaagaa gtacgaggta tcgcggcccg agctggcgcg cgacggctcg cccctcgtcg
     2041 actacgcgct gacgaccgct gagctcgcgt ccatggtcga ggaagccggc atacgtttcg
     2101 ccgacctgcg ccctggcgcg atggacctgc ctttcggctt cgccaccggc gcgggcgtgg
     2161 ctttcggcaa cgctggcggg gtggccgagg ccgtggctcg ctacgtcggc tcgcccgtat
     2221 ccccgctgac cggcgccgcg ctcgagttca aggacgaggc gcccggcggc ggtatccgcg
     2281 ccgccacgat aatcgccggc ggccgcgagc tgaccatcgt ctcggtgcag ggcctcgcga
     2341 acgccaagac ggtggcccgg ggggtgctcg acggcacccg caaggccgac atcatcgagg
     2401 tgatggcctg cccgggcggc tgcgtcggcg gcgccggaca gccgataggc ttcaagctcg
     2461 gagccgtcgc ggaaaggcgg ctcgggctgg ccgacgcgga cggcggccgg gagctgcgcg
     2521 cgtccggctc caaccccttc ctgtcgcgct tctacgccga cgttatcggc ggggctccgg
     2581 ggtcgcagcg gtcgcacgag ctcctgcata cgcgctactc gagcaagaag cgcatccagg
     2641 acaccagcat accgctgatc cgcggcggcg gcgccgagaa ggtgacggtc aaggtctgcg
     2701 tcggtacgag ctgcttcctg cgcggcagcc aggcggtgct acgcgacgtg ctcgccgagg
     2761 tggagcgtac cgggctgcag gacttcgtcg acgtcagcgc aaccttctgc tccgaacgat
     2821 gcgacaaggg tccgaccgtt cacgtcggcg gcaagaccat ccataaggcc gacgccgagg
     2881 ccgtgatggc cgcgatccgg cccctgctgg cgcatccggc cgtggcggcg gaagcggccg
     2941 ccgtctcgtc ccgctagaac gcggacgcgg ctcctacgtc tgaaccaggc gtttccgggt
     3001 tatcctcctt gcccgggaac gcctttttat atgctagacg tcggggcggc tcgggacgct
     3061 cggccgtttc atgaagcggt aggcgaacga ggcggacagc tccggcccgg ccagccagcc
     3121 ggccagtcgc ctgagccgcc aaatcgggcg cggcatcgat atcgacgata tttcgcttgt
     3181 tatcaactac gatgttccac acnnnnnnnn nnatcgatat ccaggccccg gagatcgcgt
     3241 ccctgaggca ggcccgcgcc gtcttgacgg ccggccagcc gtgcttcact tctttcctgg
     3301 cccccgccga ggcgacgagg gagaattctg tcgccaccgg tccggggcac agcgcgcata
     3361 ccgtaatacc ccgggccttc cattcggcgg cgagccccac cgataggctc agggcgtagg
     3421 ccttgctggc cgcgtaggcg g
//
LOCUS       DAOPHV010000253         3434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_37989_length_3434_cov_3.106836, whole genome shotgun sequence.
ACCESSION   DAOPHV010000253 DAOPHV010000000
VERSION     DAOPHV010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37989_length_3434_cov_3.106836"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..631)
                     /locus_tag="PLQ29_07505"
     CDS             complement(8..631)
                     /locus_tag="PLQ29_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86532.1"
                     /translation="MDIERLRRAEASFMERYPGGFEHPEMLERGRRHRIGRLVERART
                     VLAPERFDDSERMAEDIIKTVASSSMVSIFEKPAFRDLVRSLSAKERARLCSHVFQLL
                     HGDAERGFEDLSAFLASGKMAKWTVMTVFPFYTKPTEEPFVKPTTAKEIIAYYGLEGL
                     AYRPRPYWAFYSGYRGQLRAMMGLVSPLVAPDMGSFSAFLMIGAGAW"
     gene            complement(996..2054)
                     /gene="trpS"
                     /locus_tag="PLQ29_07510"
     CDS             complement(996..2054)
                     /gene="trpS"
                     /locus_tag="PLQ29_07510"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPG86533.1"
                     /translation="MERKRILTGDRPTGKLHLGHYVGSLANRVRLQDEYECFFIIADL
                     HTLTTKPEKANIEALADNVRSAALDYLACGIDPARSVIYLQSAVPEVVELSLFFQNLI
                     TVPRLSRVPSLKEMAQGAGIEEMPFGLLGYPVLQAADILLPRAHLVPVGKDNVAHVEV
                     TREIARRFNYLYGDTFPVPDAMVSEYGSLVGTDGNAKMSKSLDNAIFLSDDERTVEKR
                     VMSMYTDPKRVSADVPGTVEGNPVFQYHDAFNPDKAEVEDLKARYRAGTVGDVEVKQK
                     LAKALNAFLDPIRERRAKYESQSGLADEVVYDGTLRMREEARATLSAAKKAMGMAAVW
                     NRVSRKAEDAKKKRAKGE"
     gene            complement(2301..2750)
                     /locus_tag="PLQ29_07515"
     CDS             complement(2301..2750)
                     /locus_tag="PLQ29_07515"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HPG86534.1"
                     /translation="MKKPLATLGVVISSIVAIVGIAGLFTPKLPGLPLILFAAIGLMH
                     TSPDFVSRFNRTPRESSYIDDLLRERSMTRRNKIRTLSVTSLLLIVLFVITDSVVARI
                     LYAVFAVGKYWFFYFYIWTKVEDGHGCPDGVDCPDDKGNAGGDDEGE"
     gene            complement(2905..>3434)
                     /locus_tag="PLQ29_07520"
     CDS             complement(2905..>3434)
                     /locus_tag="PLQ29_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPG86535.1"
                     /translation="GTSEWSAEQIRQAWEIADRRNLRKPQMEQPQYSLLFRDKVEREF
                     GRLYGDIGLGLTTWSPLASGVLSGKYATGIPDGSRLALPGYGWLKERYPESVLRAAAA
                     LGPIARELGGTPAQLAIAWCARNPNVSTVITGASRPAQLEENLGALELVPKLTTEILA
                     RIDQAMAPAAAAMRD"
BASE COUNT          553 a         1078 c         1143 g          660 t
ORIGIN      
        1 tgaaaggcta ccaggcgccg gcgcctatca tcaggaaggc cgagaacgag cccatgtccg
       61 gggcgacgag cgggctgacc agacccatca tcgccctgag ctggccgcgg tagcccgagt
      121 agaaagccca atacggccgg ggccgatacg ccaggccctc gagcccgtag tacgcgatga
      181 tctccttggc cgtcgtcggc ttgacgaagg gctcttctgt gggcttcgtg tagaatggga
      241 agaccgtcat gaccgtccac ttggccatct tgccggacgc caggaacgct gagaggtctt
      301 cgaagccccg ctcggcgtcg ccgtggagca gctggaacac gtgggagcac agcctggccc
      361 gttccttggc cgacaggctc ctcaccaggt cgcggaacgc cggtttctcg aagatcgaca
      421 ccatcgacga cgaggcgacc gtcttgatga tgtcctcggc catgcgttcg ctgtcgtcga
      481 agcgttccgg tgccaggacc gtcctggccc gctcgaccag cctgcctatc cggtgccggc
      541 ggccgcgctc gagcatctcc gggtgctcga agccgcccgg gtagcgctcc atgaaggatg
      601 cttcggctcg tcgtaaccgt tctatatcca tgcccgatac gataccgcct cagcccgttc
      661 cccggcaacg ccgcgctaaa cctcggctga tagaatttcg cgcggagatg cggagacgct
      721 aaggaagatg gctcgtagta cgaaaggcgg aacataatgt tgcaggattt ctcaagatga
      781 ccatgatgag ggataaggac taggccgcat actcaggaat tcatcatggg aatcctgctg
      841 catcttgatc atcctgtccg aatcctgatc atcctgttcc gtctttgctc tattcctttc
      901 tattctcttg gcgagcgttc ctctttcctg cctcttcgtc tcgtatcctc tcgttgccgc
      961 cttgtttact acgccgcctt gttcggtccg ccgccttact cgcccttggc tcgcttcttt
     1021 ttagcgtcct cggccttgcg ggagacgcgg ttccagacgg cggccatgcc catggctttt
     1081 tttgcggcgg acagcgtcgc gcgggcctcc tcgcgcatgc gcaaggtgcc gtcgtagacc
     1141 acctcgtcgg ccaggccgga ctgagactcg tacttggcgc gccgctcgcg tatcgggtcg
     1201 aggaacgcgt tgagcgcctt ggccagcttc tgcttgacct cgacgtcgcc caccgtgccg
     1261 gctcggtagc gggccttgag gtcctcgacc tcggccttgt ccgggttgaa ggcgtcgtgg
     1321 tactggaaca cggggttgcc ctcgacggtg cctggcacgt cggcgctgac ccgcttgggg
     1381 tcggtgtaca tcgacatgac ccgcttctcg acggtccgtt cgtcgtcaga caggaagatc
     1441 gcgttgtcga ggctcttgga catcttggcg ttgccgtcgg tgccgacgag ggagccgtat
     1501 tccgatacca tcgcgtccgg caccgggaag gtatcgccgt acaggtagtt gaagcggcgg
     1561 gctatctccc gggtcacctc gacgtgggcc acgttatcct tgccgacggg cacgaggtgg
     1621 gcgcgcggta ggaggatgtc cgcggcctgg agcaccgggt agcccaggag cccgaacggc
     1681 atctcctcga tgccggcgcc ctgggccatc tccttgaggc tcggcacgcg ggacaggcgc
     1741 ggcacggtga tcaggttctg gaagaacagc gacagctcga ccacctccgg cacggccgac
     1801 tgcaggtaga tcacgctcct ggccgggtcg atgccgcagg ccaggtagtc gagcgcggcg
     1861 gagcgcacgt tgtcggccag cgcctcgatg ttagccttct cgggcttggt cgtcagggtg
     1921 tgcaggtcgg cgatgatgaa gaagcactcg tactcgtcct gtagcctgac gcggttggcc
     1981 aggctgccga cgtagtggcc gaggtgcagc ttcccggtgg gcctgtcgcc cgtcagtatg
     2041 cgtttgcgtt ccatggtgat gctccggatg atgtcattgg ggcggcccgg gggccgatgc
     2101 ggaaacggtg gatcaggcgg gccgcctcgc ggggctccgc gaagccccgg cgcccctcga
     2161 cgaggagcgc gcgcccgcct agcgccagga ccaggagccc ggcgccggtg ggatgaggcg
     2221 agtccgcatg acgggcgcgc gaagttcgtt catacgccga ttctatcgac gatagcgggc
     2281 cgtggcaagg acgggcgggg ctattcgcct tcgtcgtcac cgcccgcgtt gcccttgtcg
     2341 tccgggcagt cgacgccgtc ggggcagccg tgcccgtcct cgaccttggt ccagatgtag
     2401 aagtagaaga accagtactt gcccacggcg aagacggcgt agagtatcct ggccacgacc
     2461 gagtcggtga tcacgaacag gacgatgagg agcagcgagg tgacggacag cgtacggatc
     2521 ttgttgcgcc gcgtcatcga ccgttcgcgt aggaggtcgt cgatgtacga cgactccctg
     2581 ggcgtccggt tgaagcgcga tacgaagtcc ggcgaggtgt gcatgaggcc tatggccgcg
     2641 aacaggataa gcggaaggcc cggtagcttc ggcgtgaata ggcccgcgat gcctacgatg
     2701 gctactatcg agctgatgac gacccctagg gtggccagcg gcttcttcat gggtatcctc
     2761 aaggatcggg tttttacagt attaattcaa gggtaccgcc attatatagc atggctttgg
     2821 ccagcgggat aggggaaaac ggttgaggcg acgatattgt cgggcaaaac cggcgatttc
     2881 ctacccctat cccgccaacg gctatcagtc gcgcatcgcg gcggcggccg gcgccatcgc
     2941 ctggtcgatc ctggccagta tctccgttgt aagcttgggc accagctcga gcgccccgag
     3001 gttctcctcg agctgggccg gacggctcgc tccggtgatg accgtgctga cgttcgggtt
     3061 gcgggcgcac caggctatgg cgagctgggc gggggtgccg ccgagttcgc gcgcgatcgg
     3121 cccgagggcc gcggcggcgc gcaacaccga ttcggggtag cgctccttga gccagccgta
     3181 gcccggcagg gccaggcgcg agccatcggg gatgcccgtg gcgtatttgc ccgacagcac
     3241 gccggaggcc aggggcgacc aggtggtcag gccgaggccg atgtcgccgt acagccgccc
     3301 gaactcgcgc tcgaccttat cgcggaacag caggctgtac tgcggctgct ccatctgcgg
     3361 cttgcgcaga ttgcgccggt cggcaatttc ccaggcctgc ctgatctgct ccgcgctcca
     3421 ctccgaggtg cccc
//
LOCUS       DAOPHV010000254         3427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_38110_length_3427_cov_3.827995, whole genome shotgun sequence.
ACCESSION   DAOPHV010000254 DAOPHV010000000
VERSION     DAOPHV010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38110_length_3427_cov_3.827995"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="PLQ29_07525"
     CDS             complement(<1..931)
                     /locus_tag="PLQ29_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG86536.1"
                     /translation="MFDPPLAPANGVRVCVIGGGGTGAALAYDLCLRGFSVTLLEKGE
                     LTSGTTGRHHGQLHSGARYAVGDRAIARECMEETLILRRIAPDAVEYNGGVFVALSDD
                     EADYAPVFTAACVESGIPAREVTLAEARAWEPAINPAARRAVWVPDGAFDAFRVPLAF
                     FAAARRLGAVIRPFVEAVGFDLTGGRVRAVFARPCGSTRDERIECDYVVSATGAWAGA
                     VGRLAGLDVPITPAPGTMVAVRGRVSDMVVSRLAPPGDGDIIVPQRGLSIIGTTQRVV
                     DDPDGILPNDDDVAFLLGRADELCPGFSKRPIHA"
     gene            complement(1021..1692)
                     /locus_tag="PLQ29_07530"
     CDS             complement(1021..1692)
                     /locus_tag="PLQ29_07530"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPG86537.1"
                     /translation="MVDVLILDSEERIKAFTHPYRMKLVHVFRETRRPMTATEVARVI
                     GDGPGRVHYHMRVLEAAGIVTVARTETVNGIVARYYEPTARHYSVGEAARGAIGGEPM
                     RDEVTRMISRRFREGLRAFLDSATSGDADGVGSGEAEACPSAFLLERTVHCDDEAWLG
                     FRDAVEALIERYAEPAPGSKRRRVFMAGTTDRPNTEREARPPRRRRAGPHDRPDWPPF
                     PELER"
     gene            complement(1845..2438)
                     /locus_tag="PLQ29_07535"
     CDS             complement(1845..2438)
                     /locus_tag="PLQ29_07535"
                     /inference="COORDINATES: protein motif:HMM:NF021612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HPG86538.1"
                     /translation="MPLIDSIAQAEVRIGSWPAALILLAVVIVAVVALALWLGLAIGR
                     RSGRLEAERSGREAEAAARDDAVRRSRAVLTGQIGEQLAPYLPGFPCDPADARFIGKP
                     IDFIAFPGASAGAPVEALFIEVKTGDSRLSAAERALREAVEAGRVRWVEYRPPVGSRA
                     PGLSSAAGLAAADTRPGPGGGPSGSAVPTKAPRRPRA"
     gene            2488..3180
                     /locus_tag="PLQ29_07540"
                     /pseudo
     CDS             2488..3180
                     /locus_tag="PLQ29_07540"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
     gene            3220..3427
                     /locus_tag="PLQ29_07545"
                     /pseudo
     CDS             3220..3427
                     /locus_tag="PLQ29_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
BASE COUNT          532 a         1203 c         1197 g          495 t
ORIGIN      
        1 cggcgtgaat cggccgtttc gagaagccgg ggcatagctc gtcggcccgt ccgagcagga
       61 aggccacgtc gtcgtcgttc ggtaggatgc cgtccgggtc gtcgacgacc cgctgcgtcg
      121 tgccgatgat cgacaggccg cgctgcggca cgatgatgtc gccgtctccg ggaggggcca
      181 gtcgtgatac gaccatatcg gagacgcggc cgcgcaccgc gaccatcgtg ccaggcgcgg
      241 gcgtgatcgg cacgtcgagg ccggccagcc gcccgaccgc cccggcccag gcgccggtcg
      301 cgctgacgac gtagtcgcac tcgatcctct cgtccctggt cgagccgcag ggcctcgcga
      361 agaccgcgcg caccctgccc ccggtcaggt cgaagccgac cgcctcgacg aagggcctga
      421 ttacggcgcc gagtcggcgc gccgcggcga agaaggccaa cggcaccctg aaggcgtcga
      481 aggcgccgtc cggcacccag accgcgcgcc tcgcggctgg gtttatggcc ggctcccagg
      541 cgcgggcctc ggcgagcgtc acctcgcgcg ccgggatgcc ggactcgacg caggccgccg
      601 tgaagaccgg cgcatagtcg gcttcgtcgt cgctcagggc cacgaacacg ccgccattat
      661 actcgacggc gtccggagcg atacgcctga ggatcagcgt ctcctccatg cactcgcggg
      721 ctatagcccg gtcgccgacc gcgtagcggg cgcccgaatg cagctggccg tggtggcggc
      781 cggtggtccc ggaagtcagt tcgcccttct cgagcagcgt caccgagaag ccgcggaggc
      841 acaggtcgta ggcgagcgcg gcaccggtgc cgcccccgcc gatcacgcag acgcggacgc
      901 cgttagcggg cgccaggggt ggatcgaaca tgatggcgag tatactggca gtcggtggag
      961 cgggcaagcg cgaccgaacc ctcgatccgc gagcgcgacc aataagattc gtaacaccgc
     1021 tcagcgttcg agctcgggaa agggcggcca atcgggccga tcgtgtggac cggcccgtcg
     1081 gcgacgcggt ggccgggcct cacgctcggt attcggtcta tcagtggtgc cggccatgaa
     1141 cacccgccgg cgcttcgagc cgggcgccgg ctcggcgtag cgctcgatca gggcctcgac
     1201 cgcgtctcgg aagccgagcc aggcctcgtc gtcgcagtgg accgttcgct cgaggaggaa
     1261 ggccgacgga caggcctctg cctccccgga acccactcca tcggcgtctc cggaggtcgc
     1321 gctgtccagg aaggcgcgga gcccttcgcg gaagcggcgg ctgatcatgc gggtcacctc
     1381 gtcgcgcatc ggctccccgc ctatcgcgcc gcgcgccgct tccccgacgc tatagtgccg
     1441 ggcggtcggc tcgtagtaac gcgccacgat gccgttgacc gtctccgtac gcgccaccgt
     1501 cacgataccg gcggcctcga gcacgcgcat gtggtagtgc acccggcccg gcccatcccc
     1561 aatgaccctc gccacctcgg tggccgtcat cggacgcctc gtctccctga acacatgcac
     1621 cagcttcatc cgatatgggt gcgtgaaggc cttgatgcgt tcttcgctgt cgaggatcag
     1681 gacgtctacc atgcgcccag cataacgttc agctttattg aacgtcaagg aaagcgtacg
     1741 atgcgggagg tgaggagccg gatgctatcc ataaggttcg caacacccta cacggctcga
     1801 gtcacgaggc gctatgccta aggttcatac cactaaggcc cgcgctaggc gcgcgggcga
     1861 cgaggggcct tcgtcggaac ggcggatcct gacggcccgc cgcccgggcc cgggcgcgta
     1921 tcggcggcgg ccaagcccgc cgcggacgag agccccggcg cccgggaccc gaccggaggc
     1981 cggtactcca cccagcgcac gcggccggcc tccacggcct cccgtagcgc tcgctcggcc
     2041 gccgacagac gcgagtcgcc ggtcttcacc tcgatgaaca gcgcctcgac gggggcccct
     2101 gccgaggcgc cggggaaggc gatgaagtct atcggcttgc ctatgaagcg ggcgtcggcc
     2161 gggtcgcagg ggaagccggg caggtacggg gccagctgct cgcctatctg cccggtcagt
     2221 accgcgcggg agcggcgcac ggcgtcatcg cgggcggccg cttcggcctc gcggccggaa
     2281 cgctcggcct cgaggcggcc cgatcggcgc ccgatggcca ggcctagcca cagcgcgagc
     2341 gccaccacgg ctacgatgac gacggcgagc aggatgagcg cggctggcca ggagcctatc
     2401 ctgacctccg cttgcgctat ggagtcgatg agcggcatac tgaggctact gaagcgcgtt
     2461 tacggcgcct cggtcaagag gaaagccatg cgagacgcgg ataccagcgc cgggacggcg
     2521 atcgacgacg ctctagggcg cgcggccgag cttgtctcgg gctcgagcta cctgatagcc
     2581 tttaccgggg ccggcgtgtc tgccgagtcg ggcataccca ccttccgtgg cccgggcggc
     2641 atctggggat cctacgacga gagccacctg gagcttggct tcttcatgcg tgagccggag
     2701 atagcctgga agacgatcaa gcagatcttc tattccttca ccctaagcgt cgagccgaac
     2761 gacgcgcatc gcgtcctggc ccgctgggag gccgccggca ggctcaggct cgtcgtcacg
     2821 cagaacatag acgggctgca ccgtcgcgcc ggctcgagga aggtggccga gttccatggt
     2881 tcctgcgacg agctggtctg catgcgctgc ggcggccgcg tcccggcgac cgccgagcta
     2941 ctcgaggccc tgccgccgcg ctgcgagtgc ggcgggctcc tgaagccggg cttcgtgttc
     3001 ttcggcgagg gcataccgga ggacgcctac gagacggcct tctccgcggc cgcgctcgcg
     3061 gacgcctgcc tcgttatcgg ctcgaccggc accgtctacc cggcggcctc gataccgcgc
     3121 gcggtcaagc gagcgggcgg ccgcgtcgtc gagatcaacc ccgagccgag cgagttcacc
     3181 gacggggtca gcgacgtgtt catccgcctg ccggccgccg aggcgatgcg caggctcgag
     3241 gcgatgctct tatgcgcgga cagggaaaac ctgctgctcg tcagcggcac gggcgaggtc
     3301 atcgagccgg acgacggcgt gctcgcgatc ggctccggcg gcaacttcgc gctggcggcc
     3361 gcgcgcgcgc tgatcgcgaa cacggcgctc tccgcgaagg agatcgccga gcgctcgctt
     3421 cacatcg
//
LOCUS       DAOPHV010000255         3425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_38154_length_3425_cov_3.131751, whole genome shotgun sequence.
ACCESSION   DAOPHV010000255 DAOPHV010000000
VERSION     DAOPHV010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38154_length_3425_cov_3.131751"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLQ29_07550"
     CDS             complement(<1..385)
                     /locus_tag="PLQ29_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86539.1"
                     /translation="MKRLSLLAVALLAVVVFAGADDTGRTESEKRAATIRYGIESQVV
                     ELLSTLKTEKDDSLKAEILDAFDASTSTKLKASILEYLAALELGDAEARAARIVAERD
                     SNAEGVVTAAFSYLRALKLRAALDEA"
     gene            complement(394..1527)
                     /gene="tgt"
                     /locus_tag="PLQ29_07555"
     CDS             complement(394..1527)
                     /gene="tgt"
                     /locus_tag="PLQ29_07555"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPG86540.1"
                     /translation="MSSSSIFRTIAKDAKSAARTGVVELPHGRVRTPVFMPVGTNATV
                     KAVSKDSLAEIGFEIILANTYHLFLRPGHELIGRAGGLHGFTGWRGNFLTDSGGFQVF
                     SLSKLRKISDEGVKFQSHIDGSRHFLTPELAVDIQTAFRSDVFMQLDVCTPWGADEKS
                     SLAALRKTTAWAARSKARWLETRGDFQGKLFGIVQGNFYKELRARSAEEILALDTPGV
                     AIGGLSVGEPFEVYADLLAYTAALLPEDKPRYVMGIGTPEYILEAVQNGVDMFDCVFP
                     TRTARNGLLFTAFGPISIKQARYTEDMGPADPDCSCKVCREYSRAYLRHLYKNDEILF
                     SMLASYHNLAFLHELVRRVRYSIEEGNFVEFKREFLSRYRSKL"
     gene            complement(1553..2644)
                     /locus_tag="PLQ29_07560"
     CDS             complement(1553..2644)
                     /locus_tag="PLQ29_07560"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPG86541.1"
                     /translation="MRRLRSSPTLDAHVALSFLPIFAISLTFFVLLLEMGDLFVNIVQ
                     YVQNETPFALIAKSMILYLPRCVSWALPIATLFSVSYTLGGMYANNELIVVFGSGISL
                     ASFVAPLLAISLVLSAGFLVFDDAVVIPSLSEKKALTKAMLKTGEPAGAADVTILGQG
                     RRIVWNVRYFDRKNTMLTGVTLVERDDGGRIASRVNAQSAVWTGTAWRFSGVRRFFWK
                     DGAMTDESLGTWEDPAFAEPPESFKGGGKPIEELRLSDAAEHIAFLERAGLPRAAQTA
                     EYYRRFAFALTPLVVTLLSAALVGRYKKNVLLMSLLISLVAATLYYVTQMVTMLLAKN
                     GSVSPAAGAFTPIIIFSALVLALFKARPA"
     gene            complement(2641..>3425)
                     /locus_tag="PLQ29_07565"
     CDS             complement(2641..>3425)
                     /locus_tag="PLQ29_07565"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPG86542.1"
                     /translation="TGAMANGVIEDVIIIDATKDGKSRVISAGEAYLLDRGDEAGVLS
                     LMLGEVFTQESDPSQPERFEYSTAERMEYNILLTSFADLSAGVSPREMSSVDVAKAIG
                     RKEAGLEAKRQARGAELEAKRSALAADYLVRSVPGARLDSSMARLKPAKDSLVALAAK
                     PLKDRSLDIYRLEYYKKFSIPAGALCFVFLAFPLGARAKKSGRAVGFGIGLLVAVAYW
                     ALLIGGQTLGLRTGFDPFLSMWAPNILVLAAAVPLLLVRRSA"
BASE COUNT          638 a         1084 c         1112 g          591 t
ORIGIN      
        1 cggcctcgtc gagggccgcc ctcagtttca gcgccctcag gtaggagaaa gccgccgtga
       61 cgacgccctc ggcgttcgag tcacgctcgg cgacaatcct ggccgcacgc gcctccgcgt
      121 cgcccaactc cagggccgcc agatattcga ggatcgaggc cttgagcttc gtactggtgg
      181 acgcgtcgaa cgcgtcgagg atctcggcct tgagcgagtc gtccttctcg gtcttgagcg
      241 tcgagagcag ctcgacgacc tgggactcga tgccgtagcg tatcgtcgcc gcgcgcttct
      301 ccgattcggt gcggccggta tcgtccgcgc cggcgaacac gacgacggcc agaagggcga
      361 cggccaggag cgataatcgt ttcatgcggg ccctcacagc ttcgaacggt atctggacag
      421 gaactcccgc ttgaactcga cgaagttccc ctcctctata ctgtatcgga cgcggcggac
      481 caattcatgc aggaaagcca gattatggta gctggcgagc atggagaaga gtatctcgtc
      541 gttcttgtac aggtggcgaa ggtaggcgcg cgagtattcc ctgcagacct tacagctgca
      601 gtcggggtcg gcaggcccca tatcctcggt gtagcgggcc tgcttgatcg agatcgggcc
      661 gaaagcggtg aacaggaggc cgttcctcgc ggttcgcgtc gggaagacgc agtcgaacat
      721 atcgaccccg ttctgaaccg cctccagaat gtactccggc gtcccgatgc ccatgacata
      781 gcgcggcttg tcctccggca ggagcgccgc ggtatacgcc agcaggtcgg cgtagacctc
      841 gaagggctcg cctacggaca gccccccgat ggccacgccg ggcgtgtcga gggccagtat
      901 ctcctcggcg ctcctggcgc gcagctcctt gtaaaagtta ccttgcacga tgccgaacag
      961 cttgccctgg aagtcgccgc gcgtttccag ccagcgcgcc ttggacctgg cagcccaggc
     1021 ggtcgtcttg cgaagcgccg ccagggagct cttctcgtcc gccccccagg gcgtgcagac
     1081 gtcgagctgc atgaacacgt cgctacggaa ggccgtctgg atgtcaacgg ccagctccgg
     1141 cgtcaggaaa tgcctggagc cgtcgatatg ggactggaac ttgacgccct cgtcgcttat
     1201 cttgcgaagc ttcgacagcg agaagacctg gaacccgccc gaatcggtca ggaaatttcc
     1261 cctccagccg gtaaagccgt ggagcccccc cgcccgcccg atcagctcgt ggcccgggcg
     1321 taggaagagg tggtaggtat tggccagtat tatctcgaag ccaatctccg ccagcgaatc
     1381 cttggatacg gccttcacgg tggcattcgt accgacgggc atgaacacgg gcgtcctgac
     1441 gcgtccgtgc ggtagctcga cgacgccggt cctggcggcg ctcttcgcat ccttcgcgat
     1501 cgttctgaat atggatgacg acgacatggg ggcctccgtt cggtccgtcg gtctaggccg
     1561 gcctagcctt gaacaaggcg agcaccagcg cggagaatat gattataggc gtgaaggcgc
     1621 ctgccgctgg cgagacgctc ccgttcttgg ccagcagcat cgtcaccatc tgggtaacgt
     1681 agtacagggt agcggcgacg aggctgatga gcaggctcat cagcagcacg ttcttcttat
     1741 agcggccgac gagggccgcc gacaggaggg tgacgacgag cggggtgagc gcgaaggcga
     1801 agcgacgata gtactccgcc gtctgggccg cccgcggcag gcccgccctt tccaggaagg
     1861 ctatatgctc ggcggcgtcg gacagccgga gttcctctat gggcttcccg ccgcccttga
     1921 acgactcggg cggctcggcg aaggcagggt cctcccaggt accgagcgac tcgtcggtca
     1981 tcgccccgtc cttccagaag aagcgcctga caccggagaa gcgccaggca gtccccgtcc
     2041 agacggccga ctgggcgttg acccgcgacg cgatgcggcc gccatcgtcg cgttcgacga
     2101 gagtcacccc ggtcaacatc gtattcttgc ggtcgaagta tcggacattc cagacgatac
     2161 ggcgcccttg gccgagtatg gttacatcgg cggcgccggc aggctcgccg gtcttgagca
     2221 tagccttggt aagcgccttc ttctcggaca gcgatgggat gaccaccgcg tcgtcgaaga
     2281 cgaggaaccc ggccgaaagg accagcgata tcgccaggag cggggccacg aaggatgcga
     2341 gcgatatgcc cgagccgaac accacgatca gctcgttgtt ggcgtacatg cccccgagcg
     2401 tgtacgagac cgagaacagt gtagcgatcg gcagggccca gctgacgcaa cgaggaaggt
     2461 acaagatcat cgatttcgcg atcagcgcga acggcgtctc gttctgcaca tactggacga
     2521 tgttgacgaa caggtcgccc atctccagga gcagcacgaa gaaggtgagc gagatggcaa
     2581 atataggcag gaacgataag gccacgtggg cgtcgagggt cggcgacgac cggaggcgcc
     2641 tcatgctgac cgccgtacga gcaggagcgg tacagccgcc gccaacacga ggatgttcgg
     2701 ggcccacatc gataggaagg ggtcgaagcc ggtccgtagg cccagggtct gcccgcctat
     2761 caggagcgcc cagtaggcga ccgccacgag gagcccgata ccgaagccta cagctcggcc
     2821 gctcttcttg gccctcgccc ccagcggaaa cgcgaggaac acgaagcata gagcgccggc
     2881 ggggatggag aacttcttat agtattcgag cctgtagatg tcgagagacc tgtccttgag
     2941 cggcttggcc gcgagcgcta ccaaggagtc cttggcgggc ttaagcctgg ccatggacga
     3001 gtcgaggcgc gcgccgggga cggagcgcac gaggtagtcg gcggcaaggg cggagcgctt
     3061 ggcctcgagc tccgcgcccc tggcttggcg cttggcctcg agcccggcct ctttcctgcc
     3121 tatggccttg gcgacatcga cgctgctcat ctcccgaggg ctgacgccgg cgctcaagtc
     3181 ggcgaagctg gtcagcagga tgttgtactc catccgttcc gccgtcgagt actcgaagcg
     3241 ttccggctgg ctcgggtcgc tctcctgggt aaacacctcg ccgagcatca gcgacaggac
     3301 gccggcctcg tcgccccggt cgagcaggta ggcctcgccg gcgctgatca cgcgcgactt
     3361 tccgtccttc gtagcgtcga tgatgatgac atcctctatg accccgttag ccatagcgcc
     3421 ggtaa
//
LOCUS       DAOPHV010000256         3421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_38228_length_3421_cov_4.893939, whole genome shotgun sequence.
ACCESSION   DAOPHV010000256 DAOPHV010000000
VERSION     DAOPHV010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38228_length_3421_cov_4.893939"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..293
                     /locus_tag="PLQ29_07570"
                     /pseudo
     CDS             1..293
                     /locus_tag="PLQ29_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-2-dehydro-3-deoxyheptonate aldolase"
     gene            341..1381
                     /locus_tag="PLQ29_07575"
     CDS             341..1381
                     /locus_tag="PLQ29_07575"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HPG86543.1"
                     /translation="MTAPEIRFGPAVGLGSLAEPATTVVVADALTSGLMPPGFDPGRV
                     VIVERGESAKSLSSLEAVYARFLELGVERDWTVVGLGGGSVSDLAGLAASTWMRGVDF
                     GFVPTTLLAMVDASVGGKNGVDFRGYKNLIGCFSQPRFVLVDTSLLDGLPDDELARGF
                     VEAIKHGVIAGGEHYAAVGRAVDRRGRIRRGELGEVVRLSVELKSAFVEADALELGER
                     RKLNLGHSFGHGVESVTGLPHGAAVAAGLATALRLAVELGGSSDDADEVIGLLERLGL
                     PTGLEAARRSSVESRDLSPSSFREAVAEALGADKKLSGGLMRFALPLAIGNVEMHAIP
                     LEELRAFARRAP"
     gene            1378..2388
                     /locus_tag="PLQ29_07580"
     CDS             1378..2388
                     /locus_tag="PLQ29_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPG86544.1"
                     /translation="MNAIGTLFRVQLFGESHGPAVGVVVDGIPAGLSLGPADFEPDLE
                     RRRAGAPGTTPRAEPDIPELLSGVYQGRTTGTPLCAIFRNADARPADYDRFSDMPRPG
                     HADLVSRIRYSGYADPRGSGHFSGRLTLALVAAGVVAKKALPGVSFGARVIEADGRVD
                     IDAAIAEAAADGDSVGAVVELRASGLRAGLGEPFFGALESLAAAALFAVPGVRGVEFG
                     DGFAAARMRGSEHNDPIVAPDGAMSKNGSGGVNGGISNGNELVVRVAMKPASTIAKPQ
                     RTWNFATGAMDELSAPGRHDACIALRGAVALEAALAVVLADLELQALARGASLPNGGI
                     PS"
     gene            2385..>3421
                     /locus_tag="PLQ29_07585"
     CDS             2385..>3421
                     /locus_tag="PLQ29_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HPG86545.1"
                     /translation="MTLESLRRDIDRIDARILSLLDERLEKGLLTRRHKSGSLDSGRE
                     AEVLGRVSARSRCLADEAFTTGLYRRIMDESKAIQDRRLSLIGFQGMHGAYGEAAARA
                     WAPEAATMPFGEFSQVFDAVLAGDVDYGIVPVENTLGGVVGQVNSILVTTELRVAGAV
                     DMAVSHCLLAPPGADHRELRTVYSHGQALSQCRRFIERNGLEGVDWFDTAGAARMIAE
                     ERPKGAAAIASRFAAELYGLEIIKDGIQDSPNNRTRFFVLAKEARQATNGPADGDHGR
                     AGDHGRAGDHGKAGDKCSAVFFADDKAGALFAVLEVFAEAGINLTRIESVPTEPGDYA
                     IPYQLLTCLVL"
BASE COUNT          513 a         1236 c         1191 g          481 t
ORIGIN      
        1 acccggacgt catcctctgc gaacggggca tccgcagctt cgaaacctat acgcgcaata
       61 ccctcgacct gtccatcgtt ccgtcggtca aggcgacgag ccatctgccg atcatagtcg
      121 acccgtcgca cggcaccggc gtgctcggcc tgatcgaacc gatgagcctg gccgccgtcg
      181 ccgcgggggc cgacgggctc gagatagaag tgcatatcga tccgcccacg gccctgagcg
      241 ataaggctca gcagctgtcg cccgaggcct tcaagcggct gatgcgcaag atagcgacga
      301 tgcggcggac gatggacgag ctggccgcgg aggacgggcg atgaccgcgc cggagatacg
      361 cttcggcccc gccgtcggcc tcggctcgct cgccgagccg gcgacgacgg tcgtcgtcgc
      421 ggacgccctg acctccggcc tcatgccgcc cggcttcgac cccgggcgcg tcgtgatcgt
      481 ggagcgcggc gagtcggcca agagcctatc gtcgctcgag gccgtgtacg cgcgcttcct
      541 ggagctgggc gtcgagcgcg actggaccgt ggtcgggctc ggcggcggca gcgtctcgga
      601 cctggcgggc ctggccgcgt ccacctggat gcgcggcgtc gacttcggtt tcgtgccgac
      661 gacgctgctc gcgatggtcg acgcctcggt cggcggcaag aacggcgtcg acttccgcgg
      721 ttacaagaac ctgataggct gcttctccca gccgcgcttc gtgctcgtcg acacgagcct
      781 gctcgacggg ctgcctgacg acgagctggc ccgcggcttc gtcgaggcca tcaagcacgg
      841 cgtcatcgca ggcggcgagc actacgccgc cgtggggcgg gcggtcgacc gacgcggccg
      901 gatcaggcgc ggggagctcg gcgaggtcgt caggctgtcg gtcgagctca agtcggcctt
      961 cgtcgaagcc gacgcgctcg agctcggcga gcggcgcaag ctgaacctcg gacacagctt
     1021 cgggcacggc gtcgagtcgg tcaccgggtt gccccacgga gcggccgtcg ccgccggtct
     1081 ggcgacggcg ctcaggctgg ccgtcgagct cggcggctcc agcgacgacg ccgacgaggt
     1141 catcggcctc ctcgagcggc tcggcctgcc gaccgggctc gaggccgcca ggcgctcctc
     1201 ggtcgagagc cgcgacctgt cgccgtcgag cttccgcgag gccgtggccg aagcgctcgg
     1261 cgccgataag aagctgtcgg gcggcttgat gcgcttcgcg ctgccgctcg cgatcgggaa
     1321 cgtggagatg cacgccatac cgctcgagga actcagggcg ttcgccagga gggcgccgtg
     1381 aacgcgatcg gtaccctgtt ccgcgtccag ctcttcggag agtcccacgg gccggccgtc
     1441 ggcgtggtcg tcgacgggat cccggccggc ctgtcgctcg gcccggccga cttcgagccg
     1501 gacctggaga ggcgtagggc cggcgcgccc ggcacgacgc cacgagccga gcccgacatc
     1561 ccggagctgc tgtccggcgt ctaccagggc aggacgaccg gcacgccgct ctgcgcgatc
     1621 ttcaggaacg ccgacgcgag gcccgccgac tacgaccgct tctcggacat gccgaggccc
     1681 ggccacgccg acctggtctc gaggatacgc tactcgggct acgccgaccc acggggctcg
     1741 ggacacttct cgggcaggct gacgctcgcc ctcgtcgctg ccggcgtcgt ggccaagaag
     1801 gccctgcccg gcgtatcgtt cggcgcgcgc gtcatcgaag ccgacggccg ggtcgacatc
     1861 gacgccgcga tagccgaggc cgcggccgac ggcgactcgg tcggcgccgt cgtcgagcta
     1921 cgcgcgtccg ggctacgggc cggcctcggc gaaccattct tcggcgcgct ggagtcgctg
     1981 gccgcggcgg ccctgttcgc ggtgcccggc gtacgcggcg tagaattcgg cgacggcttc
     2041 gccgcggccc gcatgcgagg ctccgagcat aacgacccca tcgtcgcccc ggacggggcc
     2101 atgtccaaga acggctcagg cggcgtcaac ggcggcatat cgaacggcaa cgagctggtc
     2161 gtccgcgtcg cgatgaagcc ggcctcgacc atagccaagc ctcagcgcac ctggaacttc
     2221 gctacgggag ccatggacga gttgtcggcg ccgggcaggc acgacgcctg tatcgcgctc
     2281 cgcggcgccg tggccctcga ggcggccctc gccgtcgtcc tggccgacct cgagctccag
     2341 gcgctcgccc gaggagcctc gctaccgaac ggaggaatac catcatgacc ctcgaatcgc
     2401 tcaggcggga catagaccgc atcgacgccc gcatcctgtc cctgctcgac gagaggctcg
     2461 agaaaggcct cttgacgcgc cgccacaaga gcggctcgct cgactccggc cgcgaggcgg
     2521 aggtgctcgg gcgcgtgtcc gcccgctcgc gctgcctggc ggacgaggcc ttcaccacgg
     2581 ggctgtatcg acgcatcatg gacgagagca aggccatcca ggaccgaagg ctctcgctga
     2641 taggcttcca gggtatgcac ggcgcctacg gcgaggcggc ggcgagagcc tgggcccccg
     2701 aggcggcgac catgcctttc ggggagttct cccaggtctt cgacgccgtg ctggccggcg
     2761 acgtcgacta cggcatcgta ccggtcgaga acaccctcgg cggcgtcgtc ggccaggtca
     2821 actcgatact ggtgacgacc gagctgcgcg tcgccggcgc cgtcgacatg gccgtcagcc
     2881 actgcctgct ggcgccgccc ggggccgacc accgcgagct gcgcacggtc tactcccacg
     2941 gccaggccct gtcgcagtgc cggcgcttca tcgagcgcaa cggcctcgag ggcgtcgact
     3001 ggttcgacac cgcaggcgcc gcccgcatga tagccgagga acgtccgaag ggcgcggcgg
     3061 ccatagccag ccgcttcgcc gccgaactct acgggctcga gatcatcaag gacggcatcc
     3121 aggactcgcc gaacaatcgc acgcgcttct tcgtgctcgc caaggaggct cgccaagcca
     3181 cgaacggacc ggccgacggc gaccacggca gagccggcga ccacggcaga gccggcgacc
     3241 acggcaaagc cggcgacaag tgctcggccg tcttcttcgc cgacgacaag gccggcgccc
     3301 tgttcgccgt gctcgaggtg ttcgccgaag cgggcatcaa cctgacgcgc atcgagtcgg
     3361 tgccgaccga gcccggcgac tacgccatcc cctaccagct cctgacctgt ctggtcctgc
     3421 a
//
LOCUS       DAOPHV010000257         3421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_38229_length_3421_cov_4.512181, whole genome shotgun sequence.
ACCESSION   DAOPHV010000257 DAOPHV010000000
VERSION     DAOPHV010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38229_length_3421_cov_4.512181"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..2674
                     /locus_tag="PLQ29_07590"
     CDS             74..2674
                     /locus_tag="PLQ29_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86546.1"
                     /translation="MTQFMATLSTAQAWLLAAGALVLVVVLVALAMELRFRRAVRIAA
                     TRASAEGLRVGSFMLAVRERYILRRARRGFARAPGPFPELPAALGCVERWRGTLASTA
                     SGRAASRLLEFSPETALFDCFEAALRKGGSRSSGAGKALASWMADQGEGFALRRVALS
                     GPGRDFDAAAAGAFLADRMDEVRELLGDPEWAARAMAVRILALDDAERSKKGIADSLA
                     DPHPLVRRLALQAPLAGERLAFYEEAYRIFTKDPALEVRRAAKARIMAEFPDLYAPDM
                     DALGPEEQIHVLELLDSASERDEEIAFRFLESGESEQRLAAAEHLEARGSLRPLLEQA
                     NPGDAKDFERRLSLLRNAAEFQVYGFLEAVATMETDGAFLAAARVLESGGDRAYLAPL
                     AARWFERMGSPPYPADKTAVYRAVLDAVKARGDRAACEAVARELEARRGDRELAAAVL
                     GATDGSQDRVIFDTLCRLFADPLFPLRDELREALLKQPPELVVPFTLGFLRADRSGTP
                     RALRRDALILAGQLKLAWAIQRTMETLPTLDLEEIGALAPIVVDADSKAFKRKARYIL
                     DGVDAPSRASVLAALQAAGDRSFMADIKAALADADPDVRSAAVRALAAFNEDKTLGSG
                     GIELLRDPVERVRVAAAAAIAEAGGPGAVKKLAVVLADQNEVDEVKLSLVRGLGRATD
                     AASLELLIDALGAKDGRSDELVEALSRRRSRKDLGRILERFKDATGELKARIAEAVRR
                     MGKPGEEALVDLLAEDIVSLKPFIVEALESLGYVERRIRELKHRDPALRRAAAAALSV
                     VGSRAAYRGIVLAARDPDGEVRVAVTRALERLAGPEGEALLAELQNDPDARVRKYVLW
                     AMERVRAKAL"
     gene            2707..>3421
                     /locus_tag="PLQ29_07595"
     CDS             2707..>3421
                     /locus_tag="PLQ29_07595"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86547.1"
                     /translation="MVRTTNYPAEAARVRSPRSGARTGARRGLLLAAAALCAASSSCS
                     LFMSIFYGELRTIGVSASALGSVTVEADAPNESRVDGRYAFRDGTRVTLRAEPSEGGV
                     FDSWSGGYTSYDAEFSFTVAKNAELTANFVSLHDPWLVMIYMAAANTLEADGYYDLNR
                     MELGLAEAAPALRGNLKVVALFDRISTADYLAANPGVNTSYNSSDDGDWSGARLFEVR
                     PDRDPASLASHVIPSASSSL"
BASE COUNT          507 a         1253 c         1157 g          504 t
ORIGIN      
        1 caagggcaag gccgaaccga tcaagatcta tagcgctatc gaacgtataa aaaggtaacg
       61 gagggctcgc atcatgacgc aattcatggc aacgctatcg acggcccaag cctggctcct
      121 ggcggcaggc gcgctcgtcc tcgtcgtcgt cctggtcgcc ttggccatgg agctacgctt
      181 caggcgggcc gtcaggatcg cggctacgcg agcctcggcg gaaggcctcc gcgtcggctc
      241 gttcatgctc gccgtccgcg agcgctatat actgcgccgc gccaggcgcg gcttcgcgag
      301 ggctccgggc cccttcccgg agctgccggc cgccctcggc tgcgtcgagc gctggcgcgg
      361 cacgctcgct tcgaccgcct cgggtcgggc ggcgagccga ctcctcgagt tctctccaga
      421 aacggcgctg ttcgactgct tcgaggcggc cctccgtaag ggcggctcgc gctccagcgg
      481 cgcgggcaag gccctggcct cctggatggc cgaccagggc gagggcttcg cgctcaggcg
      541 cgtggccctg tccggtccgg gccgcgactt cgacgccgcg gcggccggcg ccttcctggc
      601 cgaccgtatg gacgaggtgc gcgagctcct cggcgacccc gaatgggcgg cccgagctat
      661 ggcggtcagg atactcgccc tcgacgacgc cgagcgatcc aagaagggca tagccgacag
      721 cctggccgac ccccacccgc tcgtcaggag gctggcgctc caggccccgc tcgccggcga
      781 gcgactggcc ttctacgagg aggcgtaccg cattttcacc aaggacccgg cgctcgaggt
      841 cagacgggcc gccaaggctc gtatcatggc cgaattcccc gacctctacg cccccgacat
      901 ggacgcgctc gggcccgagg agcagatcca cgtgcttgag ctgctcgact cggcgagcga
      961 gcgcgacgag gagatagcgt tccgtttcct cgagtcggga gagagcgaac agcggctcgc
     1021 cgcggcggag cacctcgagg cgcgcggatc cctgcgcccg ctgctcgagc aggccaatcc
     1081 cggcgacgcc aaggacttcg agcgccgcct gtcgctacta cgcaacgcgg ccgagttcca
     1141 ggtctacggc ttcctcgagg ccgtcgcgac gatggagacc gacggcgcct tcctggcggc
     1201 ggcccgcgtg ctcgagtcag gcggcgaccg cgcctatctt gcgcccctcg cggcgcgctg
     1261 gttcgagcgc atgggaagcc ctccctaccc ggccgacaag actgccgtct accgcgccgt
     1321 gctcgacgcc gtcaaggccc gaggcgaccg cgcagcctgc gaggccgtag cgcgggagct
     1381 cgaggccagg cgcggcgacc gggagctggc ggccgccgtc ctaggcgcca cggacggctc
     1441 gcaggaccga gtcatcttcg atacgctgtg ccggctgttc gccgacccgc tcttcccgct
     1501 gcgcgacgag ctgcgcgagg cgctgctcaa acagccgccc gagctggtcg tgccgttcac
     1561 gctcggcttc ctgcgcgccg accggtccgg gacccctcgc gcgctccggc gggacgcgct
     1621 gattctggcc ggtcagctca agctggcctg ggccatccag cgcacgatgg agaccctgcc
     1681 gaccctcgac ctcgaggaga taggcgccct ggcgcccatc gtcgtcgacg ccgattccaa
     1741 ggcctttaag cgcaaggcgc gttacatcct cgacggcgtc gacgcgccat cccgggcctc
     1801 ggtgctcgcg gccctacagg cggccggaga ccgctccttc atggcggaca tcaaagccgc
     1861 cctggccgac gccgacccgg acgttcgctc ggccgccgtc cgcgccctgg ccgcgttcaa
     1921 cgaggacaag actctcggtt ccggcggtat cgaacttctc cgcgatccgg tcgagcgcgt
     1981 acgcgtcgcg gccgcggccg ccatagccga ggccggcggc cccggcgccg tcaagaagct
     2041 cgcggtcgtg ctcgccgacc agaacgaggt cgacgaggtc aagctgtccc tcgtgcgcgg
     2101 cctcggccgc gccaccgacg ccgcgagcct cgaactcctg atcgacgccc ttggcgcgaa
     2161 agatggtcgc tcggacgagc tcgtcgaggc gctatcgcgc cgccgctccc gcaaggacct
     2221 cggcaggata ctcgagcgct tcaaggacgc gaccggagag ctcaaggcca ggatagccga
     2281 ggccgtccgc cggatgggca agcctggcga ggaggccctc gtcgacctct tagccgagga
     2341 catcgtctcg ctcaagccct tcatcgtcga ggctctggag tcgctcggct acgtggagcg
     2401 caggatacgg gagctcaagc accgcgaccc ggccctgcgt cgggcggcgg cggccgccct
     2461 gtccgtggtc ggcagccgcg ccgcgtatcg gggcatcgtg ctcgcggccc gtgaccccga
     2521 cggcgaggtg cgcgtcgccg tgacccgcgc cctggagcgc ctggctggcc ccgagggcga
     2581 ggccctcctc gccgagctcc agaacgaccc cgacgcccgc gtacgcaagt acgtgctctg
     2641 ggcgatggag cgcgtgcggg ccaaggcgct gtaagagacc gagccgcgtg ctatagtttg
     2701 gttacaatgg taaggacgac gaactacccg gccgaggccg ctcgggtacg gagcccgcgc
     2761 tctggcgcgc gaaccggcgc gcggcgcggc ctcctgctcg cggcggccgc gctatgcgcg
     2821 gcctcgtcgt cgtgctcgct gttcatgagc atcttctacg gcgagctacg gaccatcggc
     2881 gtctccgcct cagccctcgg ttcggtgacg gtggaggccg acgccccgaa cgagtcgcgc
     2941 gtcgacggac ggtacgcgtt ccgtgacggc acccgcgtga cgctacgagc cgagccctct
     3001 gaaggcggcg tcttcgactc gtggagcggc ggctacacga gctacgacgc cgagttcagc
     3061 tttaccgtgg ccaagaacgc cgagcttacg gcgaatttcg tctcgctcca cgatccatgg
     3121 ctcgtgatga tttacatggc cgccgccaac accctcgagg cggacggcta ctacgacctg
     3181 aaccgtatgg aactgggact ggccgaggcc gcgccggccc tgcgcggcaa cctcaaggtg
     3241 gtcgcattgt tcgaccgcat ctccaccgcc gactacctgg ccgccaatcc gggagtcaat
     3301 acctcctaca actcgtccga cgacggcgac tggtcgggcg ccaggctctt cgaggtcagg
     3361 ccggacaggg acccggccag cctggcctcc cacgtgatcc cgagcgcctc gtcgagcctc
     3421 g
//
LOCUS       DAOPHV010000258         3417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_38298_length_3417_cov_3.079714, whole genome shotgun sequence.
ACCESSION   DAOPHV010000258 DAOPHV010000000
VERSION     DAOPHV010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38298_length_3417_cov_3.079714"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(884..2218)
                     /locus_tag="PLQ29_07600"
     CDS             complement(884..2218)
                     /locus_tag="PLQ29_07600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPG86548.1"
                     /translation="MDIFKSLLKISLPVMFGSAVETLYNLTDAFFLGRLGTAEVGAPS
                     IAFSIVFFVTVFGIGLSGAGTTLIAQSKGKRDEAKMNHYMNQTTSLLIVASVTLAAVG
                     SIVAAPLLRLLNTPGDVFGYALVYMRIVFLGIPFVFMYFALQAAFVAIGDTVTPIKVH
                     LLAVALNVVLDPLLIFGLGPCPALGVAGAALATILSQAVGAALSLLVLRKGRSGLRLS
                     ASELKPSPKAWRLLLRIGLPASVGQGLSAFGFTVLQSVVNMFGTAAIAAFGVGNRIMN
                     LFDIPVHGIANGTTSLVGRAMGAKDERLAMSVVKNSLWMVLLFEIPLLSMAFVYGGGL
                     VRLFVADPEAIRLGDIMFKVVTPSLLTFGLFFAMSGAFQGAGDTKIILFLSVFRLWAV
                     RVPLAYFLAYRTALGPLSIWVAMFASNFLTAGIGFAYFLSGRWKRALDPDAI"
     gene            2321..>3417
                     /locus_tag="PLQ29_07605"
     CDS             2321..>3417
                     /locus_tag="PLQ29_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HPG86549.1"
                     /translation="MKIWLKYLIAIAVGVTAGLVIPFGDGAVLDAVAGAALNTGRYVL
                     LPLCFASMAVAAFELHDEKRLWIVWLKTAGFATAAVVALSLIGLAGAYALSPGRIPLS
                     ADAAPAVGSVPSPLAVLSAVMPPDALGTLSSFDFLLPSSLFAMILGIGLAFDKAASKP
                     VAAMLDSASRILWQVNSFFVELLPLPLIVAAAARFVAVGRIPRLAVFGPLFAAVGAET
                     AFVVLILLPATLWFVDRKTNPFKTLFAMLAPALAGAVAGQTYLQAGVAAKHLKESLGV
                     RRRAGAVSLPVALAFGRAGTAMVTTTAFVAILNSYSNLGLGSGAVLWMLVSAPIAALL
                     LGATPGLGPIVALTSLCASYGRGFESGYILLV"
BASE COUNT          662 a         1072 c         1032 g          651 t
ORIGIN      
        1 ggcggaaggt acgccgtgga tacctggtag cacagccgca acgggtcagt gccgcccttc
       61 cgtatcgttc tgcaaccccg gccggttaag gtggggcaat tacgccagcg tacggaacag
      121 tcccaatttg atccgcttat gcggattggt tcaaagaccg atcgacccaa tcgaccatat
      181 agtcaggaaa tcccgtcagt tcgtcggggt tcccggatcg agcacaccat cgacgcattg
      241 tccgatcgtc gaacgtatgg acaatgctta gcagcttgcg ggggccagcg tcgaaccggt
      301 ccatgacttc ccaaagctgc tcgcgagtca ggaagcgaat ttttggatta cgaacctcag
      361 gcgatagcct cgaacattct ccatttacac caatgacgat aaatttgctg attattgacg
      421 aaaaatcgca ttgatttgag ctggattcat gatgcagaaa tgaaaccatc gccgcaggag
      481 ccaagctatc gactttcctg cgcacggcga tagccctagc ctgttcaggc tggcgggggc
      541 gttctttttc tggacctaca ggacggtcaa tccacatatc tataatgttt ccactcgttg
      601 cttatttcgt caagtagaat cccttgtggt tttatttgtt actgacgcac tggaattgtt
      661 acttgacatg gcaaatttca tggtgtattt tggtgtttca ggttgtttcg gtctatccga
      721 gacactacgc ctatttactt gcgcagtaaa caaaaggcaa cacggagaaa cgggcagaaa
      781 cattgatttg gggcgtctcc taagcaatag gtagtgggtt cgattcccgc ccggatcacg
      841 atacgacgag gccgcccgat gggcggcctt ttttacagcc tcgctagatc gcgtcgggat
      901 cgagcgcgcg cttccatcgg ccggacaaga agtacgcgaa acctatcccg gccgtcagga
      961 agttcgacgc gaacatggcc acccagatcg acagcggccc gagcgccgtc ctataggcca
     1021 ggaagtacgc cagcggtacg cggacggccc atagcctgaa caccgacagg aataggatga
     1081 tcttcgtgtc gccggcccct tgaaaagccc cgctcatcgc gaagaagagg ccgaaggtca
     1141 acagcgacgg ggtgaccacc ttgaacatta tatcgccgag tcggattgcc tcggggtcgg
     1201 cgacgaacag ccggaccaag ccccctccgt agacgaacgc catcgatagt agcggaatct
     1261 cgaacagtag caccatccac agcgagttct tgacgacgct catcgccagg cgctcgtcct
     1321 tggcgcccat cgcacgcccg accaggctcg tcgtaccgtt ggctatgccg tgtaccggga
     1381 tatcgaagag gttcatgata cggttgccga cgccgaaggc cgcgatcgcg gccgtaccga
     1441 acatattgac cacgctttgg agcacggtga agccgaaggc cgacaagccc tggccgacgc
     1501 tggccggtag gcctatcctg agcagcagtc tccaggcctt gggcgacggc ttgagttccg
     1561 acgccgaaag gcgcaggccg gagcggccct tccgcagtac cagcagggac agggcggcgc
     1621 cgacggcctg cgacagaatg gtggccaggg cggctccagc gacaccgagt gccgggcagg
     1681 ggccgaggcc gaagatcaac aggggatcca agaccacgtt cagcgcgacg gcgagcaggt
     1741 ggaccttgat cggggtcacc gtgtccccta tcgccacgaa cgccgcctgg agcgcgaagt
     1801 acatgaacac gaaggggatg ccgaggaaga cgatgcgcat gtacacgagg gcgtagccga
     1861 atacgtcgcc cggcgtattg agcaggcgca ggaggggcgc cgcgacgatg gagccgacag
     1921 ccgcgagcgt caccgaggcg acgatcagca gcgacgtcgt ctggttcata taatggttca
     1981 tcttggcctc gtcgcgcttg cccttcgact gggcaatgag cgtcgtccct gcgcctgaga
     2041 ggccgatgcc gaacacggtg acgaagaaca cgatgctgaa cgcgatcgac ggggcgccga
     2101 cctccgcggt gccgagccgt ccgaggaaga acgcgtcggt caggttatag agggtctcga
     2161 cggcgcttcc gaacatgacc ggtaacgaga tcttcaggag cgatttgaaa atatccaaag
     2221 gcggagcctt gatcgtcatg gctccgcata ctactaccag gacggatcga gaaacaagcg
     2281 tcgccttgac ggtgaaaccg gcctcaggta tgctcccgca atgaagatct ggcttaagta
     2341 ccttatagcc atcgccgtcg gcgtcaccgc cgggctcgtc ataccgttcg gcgacggggc
     2401 cgtactggac gccgtcgcag gcgccgcgct caataccggg cgctacgtgc tactgccgtt
     2461 gtgcttcgcg tcgatggccg tggcggcctt cgagctacac gacgagaaac gcctctggat
     2521 cgtctggctt aagaccgcgg gcttcgcgac ggcggcggtc gtggcgctat cgctcatcgg
     2581 gctggccggc gcctacgcat tgtctccggg tcgcataccg ctatccgccg acgcggcgcc
     2641 cgcggtaggc tcggtcccat cgccgctcgc cgtcctgtcg gccgtaatgc cccccgatgc
     2701 gctcggcacc ctctcgtcgt tcgattttct gctcccatcc tcgctgttcg cgatgatact
     2761 cggtatcggc ctagccttcg ataaagccgc cagcaagccg gtggccgcca tgctcgactc
     2821 ggcgtcgcgg atactctggc aggtcaacag cttcttcgtc gagctgctac cgctaccgct
     2881 catcgtcgcg gccgcggcca ggttcgtcgc cgtcggtcgc ataccgaggc tcgccgtctt
     2941 cggccccctg ttcgccgccg tcggcgccga gacggccttc gtcgtcctca tcctgctacc
     3001 cgcgacgctc tggttcgtcg accgtaagac gaacccgttc aagaccctgt tcgcgatgct
     3061 agccccggcc ctcgccggcg ccgtggccgg ccagacctac ctccaggccg gcgtcgccgc
     3121 caagcacctc aaggagagcc tcggcgtgag gaggagggcc ggcgcggtat cgctaccggt
     3181 cgccctggcg ttcggccgcg ccggcacggc gatggtcacg acgacggcct tcgtcgcgat
     3241 actcaattcg tactcgaacc tcgggctcgg ctccggtgcg gtgctatgga tgcttgtatc
     3301 cgccccgatc gcggcgctac tcctcggggc gaccccggga ctcggcccca tcgtcgcctt
     3361 gacgagccta tgcgcgtcct acggccgcgg tttcgagtcc ggctatatcc tgctcgt
//
LOCUS       DAOPHV010000259         3411 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_38395_length_3411_cov_4.435340, whole genome shotgun sequence.
ACCESSION   DAOPHV010000259 DAOPHV010000000
VERSION     DAOPHV010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38395_length_3411_cov_4.435340"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PLQ29_07610"
     CDS             complement(<1..351)
                     /locus_tag="PLQ29_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPG86550.1"
                     /translation="MNGGSDIVGLLRGMPFIGELDDEALSAIASTASLVSAAEGDVFY
                     CEDGDSYDVYFVVSGVVAIWLKLPNYERYSELLTLRSGSLFGALSFLDGARRDMRTTA
                     RERSLVLRFDGPLLK"
     gene            complement(348..3350)
                     /locus_tag="PLQ29_07615"
     CDS             complement(348..3350)
                     /locus_tag="PLQ29_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPG86551.1"
                     /translation="MAKISSIKSGERRNATILFADLQGFTALSERMDPEEMDALMTRV
                     FGAFEEIIVSRGGVVEKYIGDALVAVFGVPELHEDDPQRALESALAFLERNRALEGEL
                     APRGATLSFRIGVHSGLIATGDRGSFKVVTGHPMAIAQRLQTAAEPDRVLVSDAVKER
                     CEADFDFEGPLRLAVKGASDELVAWRLVGQAQEGSRDQGPFVGRRDELEELLRLYVRH
                     DPRATSGMYLVGEAGIGKSRLAHALMERVRRFPEFGSPVLSAKAQRFRPLRYAVVVDL
                     VLDYLGVDFQSSPEAIASALAAQPGVERAHAELFASLLSPDAPDARLRDSEIVLALFS
                     IFSALMGRYERAVYPAFLFIDNAQAMDRQSTEFIAYYLKSAPCRPFALLAGREHGQSM
                     RDAFPELKALRLYPLARDEAAALASAYWPQVPERLLEAVLAQAGGNPLFIREYALFAR
                     KHRDLSSLPGTIQNMFIASLERFEPAARELATACSVFLLNFDTEEAARVYEAAGGDPT
                     TVPASLEALCADGVLVQSGERFAFSLDVLRKALYATILNHNKRILHGVVADIMLSYPS
                     RNRFRLIRHLMKAERWLEAADVIRSDPDRNYGYEYLSIIDALYKRLAKTDPDAAVQLL
                     ITKSALNFNAGKIDEAEEDLKRVMRIAVNERNPLCMGFAYHMICAHNVMASSFQKARF
                     TGQKALHYYRRSGLAARSVQNVVRHVALSEIQRGNFEEARQMVESTASIPGRDEFEYA
                     SALAEERLYSGDYRGALAAIEAVGDRNDGSYGAVARFFGNDLRLKVLWQLCDFRALGP
                     AARALLDSGGLSDAIVSQAHAMLAASATLYGDAKAARESFMQAEYYADKARNDYDRLE
                     SSRTLALCHFVAGNARKAETAAREALILGLRHSCYYPTFTALMLLVQIASARGDDDEA
                     GFFLAEASYFFSTGFLLSNKDAIIYYYYAGASAADESSARSRGIARRLLEEEKARIGD
                     PELVANFLASRGFGEIEAALDGVRGDGP"
BASE COUNT          543 a         1115 c         1213 g          540 t
ORIGIN      
        1 cttgagcagc ggcccgtcga agcgcagcac cagcgagcgc tctcgcgcgg tcgtcctcat
       61 gtcgcgtcgg gcgccgtcga ggaaggacag ggcgccgaac agcgagccgg agcgcagcgt
      121 cagcagctcc gagtagcgct cgtagttcgg cagcttgagc cagatggcca cgacgcccga
      181 gacgacgaaa tagacgtcgt aggagtcccc gtcctcacag tagaacacgt cgccctctgc
      241 ggcgctcacg agcgatgcgg tcgacgcgat ggctgacagc gcctcgtcgt cgagctcgcc
      301 gatgaacggc atgccccgta gtagtccgac tatgtccgat ccgccgttca tggcccgtcc
      361 ccccgtacgc cgtcgagcgc ggcttcgatc tccccgaagc cgcgcgaagc caggaagttg
      421 gcgacgagct cggggtcgcc tatcctggcc ttctcctcct cgaggaggcg cctggctatc
      481 ccgcggctac gggccgacga ctcgtcggcc gcgctggcgc cggcataata gtagtatatg
      541 atcgcgtctt tattcgacaa gaggaaaccg gtggagaaga agtacgacgc ctcggccagg
      601 aagaagccgg cctcgtcgtc gtcgcctcgg gccgaggcta tctgcacgag cagcatgagc
      661 gcggtgaagg tagggtagta gcacgagtgc ctgagcccga ggatcagggc ctccctggcg
      721 gcggtctcgg ccttgcgagc gttgccggcc acgaagtggc acagggcgag ggtcctggac
      781 gactcgaggc ggtcgtagtc gttgcgcgcc ttgtcggcgt agtactcggc ctgcatgaag
      841 gactcgcgcg cggccttggc gtcgccgtac agggtggccg aggcggcgag catcgcgtgg
      901 gcctgcgaga cgatcgcgtc gctcaggccg cccgagtcga gcagcgccct ggccgccggc
      961 ccgagggccc ggaagtcgca gagttgccag agcaccttga gcctgaggtc gttgccgaag
     1021 aagcgggcca cggcgccgta gctgccgtcg tttcggtctc cgaccgcctc gatggcagcg
     1081 agcgcgcccc gatagtcgcc ggagtacagg cgctcctccg ccagggcgct cgcgtactcg
     1141 aattcgtcgc ggcccgggat cgaggcggtg ctctcgacca tctggcgggc ctcctcgaag
     1201 ttgccgcgct ggatctccga tagcgccacg tggcgcacga cgttctgcac gctcctggcg
     1261 gccaggccgg agcggcggta gtagtgcagg gccttctggc cggtgaagcg ggccttctgg
     1321 aacgacgagg ccatcacgtt gtgggcgcag atcatgtggt aggcgaagcc catgcacaga
     1381 gggttgcgct cgttgaccgc gatacgcatc acgcgcttca ggtcctcctc ggcctcgtcg
     1441 atcttgccgg cgttgaagtt gagcgcgctc ttggtaatca ggagctgtac ggcggcgtcg
     1501 ggatcggtct tggccaggcg cttgtacagc gcgtcgatga tggataggta ctcgtagccg
     1561 tagttgcgat ccggatccga gcgtatcacg tcggccgcct cgagccagcg ttcggccttc
     1621 atcaggtggc ggatcagcct gaagcggttt ctggacgggt aggacagcat gatatcggct
     1681 acgacgccgt gcaaaatgcg cttgttatgg ttgaggatgg tcgcgtacag ggcctttctg
     1741 agcacgtcga gcgagaaggc gaagcgctcg cccgactgga cgagaacgcc gtcggcgcac
     1801 agggcctcga gcgaggcggg caccgtggtc ggatcgccgc cggccgcctc atagacgcgc
     1861 gcggcctcct cggtgtcgaa gttcagcagg aacaccgaac aggccgtggc cagctcgcgc
     1921 gcggccggct cgaagcgctc gaggctcgct atgaacatgt tctggatcgt gccgggcagg
     1981 ctggacaggt cgcggtgctt gcgggcgaac agcgcgtact cccggatgaa cagcgggttg
     2041 cccccggcct gggctagcac tgcctcgagc aggcgctcgg gtacctgcgg ccagtaggcc
     2101 gaggcgagcg cggccgcctc gtcgcgggcg agcgggtaga ggcgcagcgc cttgagctcg
     2161 gggaacgcgt cgcgcatcga ctggccgtgc tcccgcccgg ccagcagcgc gaagggccgg
     2221 cacggggcgg atttcaggta gtacgcgatg aactccgtcg attgccggtc catcgcctgg
     2281 gcgttgtcga tgaacaggaa ggccggatag acggcgcgct cgtagcgccc catcagcgcc
     2341 gagaagatcg agaacagcgc cagcacgatc tccgagtccc tgagccgggc gtcgggggcg
     2401 tccggcgata ggagcgaggc gaacagctcg gcgtgggccc gctcgacccc gggctgcgcg
     2461 gccagggccg aggctatggc ctcggggctc gactggaagt cgacgcccag gtagtcgagc
     2521 accaggtcga cgacgacggc gtagcgtagc ggcctgaagc gctgcgcctt ggccgacagc
     2581 accggcgagc cgaactccgg gaagcggcgc acccgttcca tgagcgcgtg ggccaggcgg
     2641 ctcttgccta tgccggcttc gccgaccagg tacatgcccg acgtcgcgcg cgggtcgtgc
     2701 cgtacgtaga gcctgagcag ctcctcgagc tcgtcgcgcc ggccgacgaa gggcccctgg
     2761 tcgcgcgagc cttcctgggc ctggccgacg aggcgccagg cgacgagctc gtcgctcgcg
     2821 cccttgaccg ctagccggag cggcccctcg aagtcgaagt cggcctcgca gcgctccttg
     2881 accgcgtccg agacgagcac ccggtccggc tcggcggcgg tttggaggcg ctgggctatg
     2941 gccatcgggt gcccggtgac caccttgaac gagccgcggt cgccggtagc tatgaggccg
     3001 gagtggacgc cgatcctgaa cgacagcgtc gccccgcgcg gcgcgagctc gccctcgagc
     3061 gcgcggttcc gttcgaggaa ggcgagcgcc gactcgagcg cgcgctgcgg gtcgtcctcg
     3121 tgcagctccg gtacgccgaa gaccgcgacg agggcgtcgc ctatgtactt ctccacgacg
     3181 ccgccccgcg agacgatgat ctcctcgaag gcgccgaaga ccctggtcat cagcgcgtcc
     3241 atctcctcgg gatccatgcg ctccgacagc gcggtgaagc cctggagatc ggcgaagagt
     3301 atggtcgcgt tcctgcgctc gcccgatttt atggagctga tcttggccat ggcccgatac
     3361 tctagcctaa aagcgccgcg cttgaaagcg atatggcctc ggccacccgt a
//
LOCUS       DAOPHV010000260         3384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_38783_length_3384_cov_4.091919, whole genome shotgun sequence.
ACCESSION   DAOPHV010000260 DAOPHV010000000
VERSION     DAOPHV010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38783_length_3384_cov_4.091919"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PLQ29_07620"
     CDS             <1..404
                     /locus_tag="PLQ29_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86552.1"
                     /translation="STTSVFEDRAALRVRSRDHAALKNAYESIFRRRWPTIAKELEPR
                     LGPLSWKALASSGQSERELPGRFGDSGSGGLKLLLSNAAGQPAGFLWMRGSGTEPVFR
                     VMADVRGADATLEGLLLRHHTEMVTEADSIA"
     gene            456..1106
                     /locus_tag="PLQ29_07625"
     CDS             456..1106
                     /locus_tag="PLQ29_07625"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HPG86553.1"
                     /translation="MGLRGELFSTRMACDGRTYFFNVKENRMGDLFLSIVESKPTETG
                     DFDRRSIVVFQDNADEFLKAFQKSLDAMNEAAPSERSKPPRTRREAMAPRDDGREAER
                     RLSDDERPRHYIDTGKRRDKAAAGERGRPDRDASGRAVPGRAASGRDDARRDSVRRDI
                     ERATGRSSAGPNDDRRTRTTGRVLRVKKAVKAAPAVPEKKPAGKRLTVKKVPKKDE"
     gene            complement(1137..1742)
                     /locus_tag="PLQ29_07630"
     CDS             complement(1137..1742)
                     /locus_tag="PLQ29_07630"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPG86554.1"
                     /translation="MSGRAGQGELFGPPDVSSYRVVCGIDEAGRGPLAGPVSAAAVIL
                     PDDFPLRLLDDSKALSARARDAAYAVIVERARWANEWAWPSEIDGLNILGATMLAMRR
                     AFSRLGAAPDLVVVDGNRDPGLPCACRPMVKADAIVPAVMAASIVAKVARDRLMERYD
                     WLYPEYGYARHKGYPTAAHRAACRALGPSPVQRLSFDYSKE"
     gene            complement(1739..>3384)
                     /locus_tag="PLQ29_07635"
     CDS             complement(1739..>3384)
                     /locus_tag="PLQ29_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPG86555.1"
                     /translation="DPGRLRQIVVNLFKNAVKFTHAGEVVVAAKSVPRKDGPASLSLS
                     VADSGIGVPDAVKGRLFTPFTQESAGSKTRGGTGLGLAISRHLVEAMGGRIWLEPNQP
                     RGSRFCLELPLGEPTDSPPRPPRPPRGRLKPARVLVVDDHPMALAQAMRLAASFGLRA
                     DGATSGDEALAAMRSAAGVRKPYAVCLVDQNMPGMDGWRLGAEVTADRQINGSRLVLL
                     APEGAIGPDAKMKLLQWFNGYAAKPINPDELYEVLERSLADEVDLPSADETEPTAAAV
                     EAPAPAEAAEAPAPAARLELTVLLAEDHPVNRELFAALLRKLGCEVGLASDGVEALEL
                     ASRGDYDMVLMDIFMPRMNGYEAAKALRERGYSRPIIAVTASALKGERDKCIEVGMND
                     VLVKPFKKSDLATTLGFWAEKASEAEAPVYATAPEANPGGRESATLDFDGLVETFLGQ
                     RGKVLELLARFSTKTRSQLADLEAAAAAGDAKTLREIAHSIKGAAWSLSARALGDVAM
                     TIEKAAADGDAQAAAASLGELGERFAEFESIAKFYSERS"
BASE COUNT          508 a         1230 c         1172 g          474 t
ORIGIN      
        1 tctcgacgac gagcgtcttc gaggataggg ccgccctgcg cgtgcgatcg cgcgaccacg
       61 cggccctcaa gaacgcctac gagtccatct tccgccgccg ctggccgacc atcgccaagg
      121 aactcgagcc gaggctcggg ccgctgtcct ggaaggcgct cgccagctcc ggccagtcgg
      181 aacgcgagct gccgggccgc ttcggcgact cgggctcggg cggcctcaag ctgctcctgt
      241 cgaacgccgc cggccagccg gccgggttcc tgtggatgcg cggctcgggc acggaaccgg
      301 tgttccgcgt gatggccgac gtgcgcgggg ccgacgcgac gctcgaaggg ctcctcctcc
      361 ggcaccacac ggagatggtg accgaagcgg actctattgc atagaaccgc ataaagcgtt
      421 agtatccgca agtgtattca tagggagggg aatcgatggg ccttcgtgga gagctctttt
      481 caacgcgcat ggcatgcgac ggcaggacgt acttctttaa cgtcaaggaa aaccgcatgg
      541 gcgacctctt cctctcgatc gtcgagagca agcccaccga gaccggcgac ttcgatcgtc
      601 ggtccatcgt cgtgtttcag gacaacgccg acgagttcct caaggccttc cagaagtcgc
      661 tcgatgcgat gaacgaggcc gctccgtccg agcgctcaaa gccgccccgg accaggcgcg
      721 aggcgatggc cccgcgcgac gacggcaggg aggccgagcg ccgcctgtcc gacgacgagc
      781 ggccgaggca ctacatcgac accgggaaga ggcgcgacaa ggccgccgcg ggcgagcgcg
      841 gccgcccgga ccgcgacgcg tctggacgcg ccgtgcctgg acgcgccgcg tctggacgcg
      901 acgacgctcg ccgcgattcc gtgcgccgcg atatagagag agccaccgga cgctcctcgg
      961 ccggcccgaa cgacgaccgc cgcacccgca ccaccggtcg cgtgctccgc gtcaagaagg
     1021 ccgtcaaggc ggcgcccgcg gtgcccgaga agaagccggc cggcaagcgc ctgacggtca
     1081 agaaggtccc gaagaaggac gaatagcccc ggtcgccgcg gggccgggcc ccgcgatcac
     1141 tccttggaat agtcgaagct tagccgctgc accggggacg gccccagggc tcggcaggcg
     1201 gcccggtgcg cggccgtcgg gtagcccttg tggcgcgcgt agccgtactc cggatacagc
     1261 caatcgtagc gctccatcag ccggtccctc gcgaccttgg cgactatcga cgccgccatc
     1321 acggccggca cgatggcgtc cgccttgacc atcggcctgc acgcgcaggg caggccagga
     1381 tcccggttgc cgtcgacgac gaccaggtcg ggcgccgcgc cgagccgcga gaaggcccga
     1441 cgcatcgcca gcatcgtcgc ccccaggatg ttcagcccgt cgatctcgga aggccaggcc
     1501 cactcgttgg cccagcgcgc ccgctcgacg atcacggcgt aggccgcgtc gcgggccctg
     1561 gccgacaggg ccttggagtc gtcgagtagc cgtagcggaa agtcgtcggg caggatgacg
     1621 gcggccgccg agaccggacc ggccagcggg ccccgccccg cctcgtcgat gccgcagacg
     1681 acgcggtacg acgacacgtc gggcggcccg aagagctcac cctgccccgc cctcccgctc
     1741 acgaacgctc cgagtagaac ttggctatcg actcgaattc ggcgaagcgc tcgcccagct
     1801 cgccgagcga ggcggccgcc gcctgggcgt cgccgtcggc ggccgccttc tcgatcgtca
     1861 tggccacgtc gccgagggcc ctggccgaca ggctccaggc ggcgcccttg atggagtggg
     1921 ctatctcgcg gagcgtcttg gcgtcgccgg cggccgcggc ggcctccagg tcggcgagct
     1981 gcgaccgggt cttggtcgag aagcgcgcga gcagctcgag taccttgcct cgctggccga
     2041 ggaaggtctc gacgaggccg tcgaagtcga gcgtcgcgct ctcccggccg cccgggttcg
     2101 cctccggcgc cgtcgcgtag accggagcct cggcctcgct ggccttctcc gcccagaagc
     2161 cgagagtcgt cgccaggtcg gacttcttga acggcttgac caggacgtcg ttcatcccga
     2221 cctcgatgca cttgtcgcgc tcgcccttga gggcgctcgc cgtcaccgcg atgatgggcc
     2281 tcgagtagcc gcgctcgcgc agggccttgg cggcctcgta gccgttcatc ctcggcatga
     2341 agatgtccat caagaccatg tcgtagtcgc cgcgcgaggc cagctcgagg gcctcgacgc
     2401 cgtccgaggc cagaccgacc tcgcaaccga gcttgcgcag cagggcggcg aacagctccc
     2461 ggttcaccgg gtgatcctcg gcgagcagca cggtcagctc gagccgggcc gccggggcgg
     2521 gagcctcggc agcctcggcg ggcgccgggg cctcgacggc cgcggccgtc ggctcggtct
     2581 cgtcggccga aggcaggtcc acctcgtccg ccagcgagcg ctcgagcacc tcgtacagtt
     2641 cgtccggatt gatcggcttg gccgcgtagc cgttgaacca ttgcagcagc ttcatcttgg
     2701 cgtccgggcc gatggcgccc tccggagcca gcagcacgag gcgcgagccg ttgatctgcc
     2761 tgtcggcggt tacctccgcg ccgagccgcc agccgtccat gcctggcatg ttctggtcga
     2821 cgaggcagac cgcgtacggc ttcctgacgc ccgccgcgga ccgcatcgcg gcgagcgcct
     2881 cgtcgcccga ggtcgcgccg tccgccctga gcccgaacga ggccgccagt cgcatcgcct
     2941 gggcgagcgc catggggtgg tcgtcgacga cgagcacgcg cgccggcttg agcctgcccc
     3001 gaggcggcct gggcgggcgc ggcggagaat cggtcggctc gccgagcggc agttcgaggc
     3061 agaagcgcga cccgcgcggc tggttcggct cgagccagat cctgccgccc atcgcctcga
     3121 ccaggtggcg cgagatggcc agcccgagcc ccgtgccgcc cctggtcttg gagccggcgc
     3181 tctcctgcgt gaacggcgtg aacagcctgc ccttgacggc gtcgggcacg ccgatcccgc
     3241 tgtccgccac cgacagcgac agcgaggcgg gcccgtcctt cctcgggacg gacttcgccg
     3301 cgacgaccac ctcgccggca tgcgtgaact tgaccgcgtt cttgaacagg ttgacgacga
     3361 tctgcctgag ccggccgggg tcgc
//
LOCUS       DAOPHV010000261         3382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_38812_length_3382_cov_3.610460, whole genome shotgun sequence.
ACCESSION   DAOPHV010000261 DAOPHV010000000
VERSION     DAOPHV010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38812_length_3382_cov_3.610460"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1687)
                     /locus_tag="PLQ29_07640"
     CDS             complement(<1..1687)
                     /locus_tag="PLQ29_07640"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPG86556.1"
                     /translation="MDLTEAVRLDASWAIYWNRFVDPAAFEVGEAPLPDAYVPMPALW
                     TSYGPGFDSVGYATLQLSLVGLEPGRRYGLKASAFQSAASLYAGGRLVQAHGRPARTA
                     ADEEPAWRSLVSPLEADETGRLELVLHVSNFSDRSGGTHASLAFGDLAAVARERERAV
                     AYELFMCGAILVMGAYYLGLFAFRRKERAALWFGLLCVTLAVRVLLYDEFYLVSIFPS
                     LGFRTLFALGYLSFTAAVACVAAFVRATFPGEFPRWATIGAVAASAAYALFVIVAPTR
                     LTSLALPAFQACALAVGLACAVAIALAVARKRPGAGLFGLGFLAIFGTAVYDIFVTAG
                     VVHGAFASQVGMLAFLFALSLILTRRYAGAFSAAEALSDDLARVNKSMERFVPKEFLG
                     FLKKRSLEEIELGDSSSEEMAVLFVDVRSFSRMAEDSTPEATFSFINEYLARVGPAVR
                     DHGGFIDKYLGDGFMALFPGGAEPALRCALDVQARVAAYNLEREAQGRKPIRVGVGMH
                     AGRLMLGTIGESRRMDGTVVSDAVNVASRLEGVSKEYGLGIAASECVLKELSDP"
     gene            complement(1874..>3382)
                     /locus_tag="PLQ29_07645"
     CDS             complement(1874..>3382)
                     /locus_tag="PLQ29_07645"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPG86557.1"
                     /translation="GSALFAALYIPAALGLVGAIAAIFGFPGFLYEKVGGSVLPFTQL
                     AAVPSVFTKGLLFYGYALFIAACVVLASRAASKGLRRSPDIVIVLAGFLVQLGTTLAA
                     VVTSARSGSVLSSPLWATLRNLLVVYGLFGERLIGPLDNARHAVMDTLLDPVFVLGKD
                     EKVVDCNSAGQTLLGAGSADIIGKPARSLFERWPPEVSAALSFPEAETELDLPDRDGA
                     PSRSYRITVASSSPGDRLNAWKLLVFNDISGQKRDARDLAASNDELEFWVRQRTAALR
                     QEVAQRTAAENRLRELNEEMAKTQKEILWTLSEVVESRSRETAYHVIRVGEYARILSK
                     AYGLSAEEVDIITAAAPMHDVGKIAIPDSILSKPGLLTDDEREVMKRHTVIGKEILST
                     STRPLLRAAAIIALEHHERWDGKGYPLGKVAADISLSGRIVSLCDVFDALFTKRVYKD
                     AWPLEKVLALFKEESGGAFEPRLVDILFEHIDDFIDVTKRFKDEPAPALPLA"
BASE COUNT          585 a         1126 c         1192 g          479 t
ORIGIN      
        1 tcgggtcgct cagctccttg agcacgcact cgctcgcggc gatgcccaag ccgtattcct
       61 tggagacgcc ctcgagacgg ctggccacgt tgaccgcgtc ggagacgacc gtgccgtcca
      121 tgcgccggct ctcgcctatc gtgccgagca tcaggcggcc ggcgtgcatg cccacgccga
      181 cccggatagg cttccgcccc tgggcctcgc gttccagatt gtaggccgcc accctcgcct
      241 gtacgtcgag ggcgcaacgc agggccggct cggccccgcc cgggaacagg gccatgaaac
      301 cgtcgcccag gtacttgtcg atgaagccgc cgtggtccct gacggccggc cccaccctgg
      361 ccaggtattc gttgatgaac gagaaggtgg cctcgggcgt cgagtcctcg gccatcctgg
      421 agaacgagcg aacgtcgacg aagagcacgg ccatctcctc ggaactggag tcgccgagct
      481 cgatctcctc gaggctgcgc tttttgagga agccgaggaa ctccttcggg acgaagcgct
      541 ccatcgactt gttcaccctg gccaggtcgt cggacagggc ctcggccgcg gagaaggcgc
      601 cggcgtagcg ccgcgtcagg atcagcgaca gcgcgaagag gaaggcgagc atgccgacct
      661 gggacgcgaa ggcgccgtgg acgacgccgg ccgtgacgaa tatgtcgtag accgccgtgc
      721 cgaagatggc caggaacccg aggccgaaca gcccggcgcc cggacgcttc ctcgcgacgg
      781 ccagggctat ggcgacggcg caggccaggc cgacggccag ggcgcaggcc tggaaggccg
      841 ggagcgccag cgacgtcagc ctggtcggcg cgacgatcac gaagagcgcg taggcggccg
      901 acgccgcgac cgcccctatc gtcgcccagc gcgggaactc gccggggaag gtcgcccgta
      961 cgaaggcggc tacgcaggcg accgccgcgg tgaagctcaa gtagccgagc gcgaacaggg
     1021 tcctgaagcc gagcgagggg aagatggaga cgaggtagaa ctcgtcgtac aggagcacgc
     1081 ggacggcgag cgtgacgcac aggagcccga accagagagc cgcgcgctcc ttgcgccgaa
     1141 aggcgaagag gcccaggtag taggccccca tcaccaggat ggcgccgcac atgaacagtt
     1201 cgtaggccac cgcccgctca cgctcgcgcg cgacggcggc caggtcgccg aacgcgagac
     1261 tggcgtgggt gccgcccgag cggtcgctaa aattcgacac gtgcagcacg agctcgaggc
     1321 gccccgtctc gtcggcctcg agcggcgaga cgagcgagcg ccaggccggt tcctcgtcgg
     1381 cggccgtacg cgcgggccgg ccgtgggcct ggaccaggcg accgcccgcg tagagcgacg
     1441 cggcgctctg gaaagccgag gccttgagcc cgtagcgccg gccgggctcg agcccgacga
     1501 ggctcagctg cagggtcgcg tagccgaccg agtcgaagcc cggcccgtag ctcgtccaga
     1561 gggcgggcat cggtacgtag gcgtcgggta gcggcgcctc cccgacctcg aatgccgccg
     1621 gatcgacgaa gcggttccag tagatggccc aggaggcgtc gaggcgcacg gcttcggtca
     1681 ggtccaccgc cgtcaagtcg acgccggcct acgatggcga cggcgatggc gacggcgatg
     1741 gcgatggcga cggccaggcg gacgcgaccg cgagaatcgc cgcgactagg gatacggctt
     1801 ttttgatgct catcggggat cgactatatc acctgccgag tcgcttcgca acgaacgccg
     1861 gcccggcgcc cggtcaggcc aagggcagcg ccggggccgg ctcgtccttg aagcgcttcg
     1921 tcacgtcgat gaagtcgtcg atgtgctcga acaggatatc gacgaggcgc ggttcgaagg
     1981 cgccgccgga ttcttccttg aacagggcca ataccttctc gagcggccag gcgtccttgt
     2041 acacgcgctt ggtgaacagc gcgtcgaaca cgtcgcagag cgagacgata cgcccggaca
     2101 gactgatatc ggcggccacc ttgccgagcg gatagccctt gccgtcccaa cgctcgtggt
     2161 gctcgagcgc aatgatggcg gcggcgcgca agagggggcg cgtcgacgtg ctgagtatct
     2221 ccttgccgat gacggtgtgg cgcttcatca cctcgcgctc gtcgtcggtc aggaggcccg
     2281 gcttggacaa tatcgagtcg gggatcgcta tcttgcccac gtcgtgcatc ggcgccgcgg
     2341 ccgtgatgat gtcgacctcc tccgcggaca gcccgtaggc cttagagagt atcctcgcgt
     2401 actcgccgac gcggatcacg tggtaggcgg tctcgcggct acggctctcc accacctccg
     2461 acagggtcca gaggatctcc ttctgggtct tggccatctc ctcgttcaac tcgcgcaggc
     2521 ggttctcggc ggcggtacgt tgggcgacct cctgcctgag cgccgcggtc cgctgcctga
     2581 cccaaaactc cagctcatcg ttgctggccg ccaggtcgcg ggcgtcgcgc ttctggccgg
     2641 agatatcgtt gaacacgagc agcttccagg cgttcaagcg atcgcctggc gaactcgagg
     2701 cgacggttat cctgtagctg cggctcggcg cgccgtcgcg gtcgggcagg tcgagctcgg
     2761 tctcggcctc gggaaagctg agagcggcgg agacctcggg gggccatcgc tcgaacaggg
     2821 agcgggccgg ctttcctata atatccgccg agccggcgcc gagcagcgtt tggccggcgg
     2881 agttgcagtc gacgaccttc tcgtccttgc cgagcacgaa cacggggtcg agcagcgtgt
     2941 ccatgaccgc gtgcctcgcg ttgtcgagcg ggccgatcag gcgctcgccg aacaggccgt
     3001 agacgaccag caggttgcgc aacgtggccc acaggggcga cgacaggacg ctcccgctac
     3061 gcgcgcttgt gactacggcc gccagggtcg tgccgagctg caccaggaaa cccgccagca
     3121 cgatcacgat gtcgggggaa cggcggagcc ccttggaggc agcgcgcgaa gccaggacga
     3181 cgcaggcggc tatgaacagg gcgtagccat agaacagcag gcccttggtg aataccgacg
     3241 gcacggccgc gagctgggtg aacggcagga cgctgccgcc gaccttctcg tacaggaagc
     3301 ccgggaagcc gaagatggcc gctatggcgc cgacgaggcc cagcgccgcc ggtatgtaca
     3361 gcgcggcgaa caacgccgag cc
//
LOCUS       DAOPHV010000262         3379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_38859_length_3379_cov_3.932611, whole genome shotgun sequence.
ACCESSION   DAOPHV010000262 DAOPHV010000000
VERSION     DAOPHV010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38859_length_3379_cov_3.932611"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(574..2622)
                     /locus_tag="PLQ29_07650"
     CDS             complement(574..2622)
                     /locus_tag="PLQ29_07650"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPG86558.1"
                     /translation="MVTRNTVLGVAIAAILGAYSIWGAALDDALELSLDTGWQWAIED
                     EGGKVGDFAPLDSASFTNLERLLPSGSGFVWLRIEFTLPEALRGRDLACYLGRVTMAD
                     RTWVNDYFIGGEGRFPPEYTSEWNRVRVYTLPERALRPDAENVLSMRIWVDHEGSIAG
                     QPFIAAADNARGVAARETFWNETVNLLSAAIMLIMAAYHMMLYIRRKKERENLTFALL
                     NVASSIYLCNFFLGDIPGYPWPGMSFLWFQKIVANGMPFLLAFLLASFVKEYLGKTER
                     RIVLAARLACFIVPVVAVLSAGDYGTLRSMRLPTQLFLLPPLAYVLYLVGSSLRERRT
                     EAVALLWGFSPVALTVIVDTVLHEGLKLYDVPYFSGLGWQLVVISLLFILAGRFARSR
                     TEVEDLNVNLERKVADRTAELSRANDDLSHANLKLEKARFQAERDMKMATYVQESFYP
                     REAVKIDGWELDFHFQPMSGVSGDLYDFYTSGDGLLGLGLFDVSGHGVASGLVAMLAK
                     SIIHTQFRRGLDRSLASVMEDVNKRLVAEKGEIENYLTGALLRISGNRVEYVNAGHPS
                     LLFRQGATGKVRAVVPPESRATEGGVLGLDGLGGTFVSLRFPMATGDALLLYSDCLSE
                     GRDAMGEEFGQERIREAFAAAPATSAAALKASILASFTTFIGTADLTDDLTFVVLLRA
                     "
     gene            complement(2627..>3379)
                     /locus_tag="PLQ29_07655"
     CDS             complement(2627..>3379)
                     /locus_tag="PLQ29_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG86559.1"
                     /translation="MMLPVVFIDEKKYCVSIPSDDGFFSSVGKVIKDRNFLVFTLSDF
                     AYWVSLYFINNGLLYYITVLLGLPKETYSLLFIVMFAVSFIFYVPVGLLARKVGRRRL
                     LIVAFVIFGVLFGFCSLFGVLPLPAMAQAAIAAILASIPMAIFGILPNAMISDMAEAY
                     SIETGVHKAGVFFGFRTFMSKLGQFVGALILPSIVMIGADEAAGEIVGKSGVRMTTFF
                     ALGFCALGLVLLLAYNEKKVMASLDKGEGGAR"
BASE COUNT          688 a          992 c         1116 g          583 t
ORIGIN      
        1 ggccgagccg caggatgcgg cgagaggcgc gaaggcggcc cggaatgccg agccacgatg
       61 gcgaggcatg aagggcgaac ccgcgcgggg gagctacaaa ggccgatcgg catagccgat
      121 cggcctttgc atttatctgc gcgccaacgt gcgcaggacc gcagggggcc ggagggtgtc
      181 tcccggaatg cacggtccga aaggacggcc ttttcgtttg tgatcacctc cgtgagggtt
      241 cgaactgaaa gtctccgagc gaaggcgagg acgaggcgca tggatgcgcc gagagaagct
      301 tgaagcgggc tggaaacgag agacaggatg tcgagcgcgg ggtagtgagc ccgcccccta
      361 ctagctaccc gcgcgtcgac gcgcgcggaa tgcgacgtga gcagtgcgca agctcgcagg
      421 ggaggagaac gctgaaggcg ttcgaggtgg gatcgccctt accaggtatt gtccgaatgg
      481 gcggcctgcc cgattgcctt cggccctcgc tcgggttcgt cgagcgtcgt tgagcgacgg
      541 ggctccgcca tgatccttag tcgtgtctca ggttcaggcg cggagcagta ccacgaaggt
      601 caggtcgtcg gtaaggtcgg ccgttccaat aaaggtcgta aagctcgcga ggatcgatgc
      661 cttgagcgcg gcggccgaag tcgccggagc cgcggcgaag gcctcgcgga tacgctcttg
      721 cccgaattcc tctcccatcg cgtctcggcc ttccgagagg caatccgagt agagcagtag
      781 cgcgtcgcct gtagccatcg ggaagcgcag cgagacgaag gtgcctccta aaccatccaa
      841 cccgagcacc ccgccttcgg tggcgcggct ttcgggcggg actacggccc tgaccttgcc
      901 agtggcgccc tgcctgaata gtagagacgg atggccggcg ttaacgtact cgacgcggtt
      961 cccggaaatg cgtaggagcg ctcccgtaag gtaattctcg atctcgccct tttcggcgac
     1021 caagcgcttg ttgacgtcct ccatgaccga ggccagggat cggtccagcc cacggcggaa
     1081 ctgggtatgg atgatggact tggccagcat ggcgaccagg cccgaggcca ccccgtgtcc
     1141 tgatacgtcg aagaggccga ggcccagtag cccatccccg gaggtataga aatcgtagag
     1201 gtcgcccgaa acgccggaca tcggctgaaa gtgaaaatcc agctcccagc catcgatttt
     1261 caccgcctct ctggggtaga agctttcctg cacgtaggta gccatcttca tgtcgcgctc
     1321 ggcctggaat cgcgccttct ccagcttgag gttcgcgtgc gataggtcgt cgttcgccct
     1381 gctgagctca gccgtgcgat cagccacctt gcgttccagg ttgacgttga ggtcttccac
     1441 ctccgttctc gagcgtgcga accgtccggc caggatgaac aggaggctga tgacgacgag
     1501 ctgccacccg aggcccgaga aatatggcac gtcatagagt ttcaggcctt cgtgcaggac
     1561 ggtatccacg atcacggtca gagcgacggg agagaaaccc cagagcaggg ctaccgcctc
     1621 ggtcctgcgc tcgcgtaggg aagagcccac tagatagagc acgtaggcga gcggcggcag
     1681 caggaagagc tgcgtcggaa gccgcatcga gcgtagggtt ccgtagtccc ccgccgatag
     1741 tacggcgacg accggtacga tgaagcaggc gaggcgtgcg gccaagacga ttcgacgttc
     1801 ggtcttgccg aggtactcct tgacgaagga ggcgagcagg aaggccaaga ggaagggcat
     1861 tccgttggcc acgatcttct gaaaccagag gaacgacatc ccgggccagg gatagccagg
     1921 gatgtcgcct aggaagaagt tgcataggta gatcgagcta gccacgttca gtagggcgaa
     1981 cgtgaggttc tcccgttcct tcttccgcct gatgtacagc atcatgtggt aggcggccat
     2041 gatgagcatg atagccgcgg agaggaggtt gaccgtttcg ttccagaatg tttcccgcgc
     2101 ggccacgccg cgcgcgttgt ccgcggcggc tatgaagggc tggccggcta tcgaaccctc
     2161 gtggtcaacc catatcctca tgctaaggac gttctccgcg tcgggcctca gggctcgctc
     2221 gggcaacgta tagactcgga ccctgttcca ttcgctcgta tactcgggcg ggaaacggcc
     2281 ctctcctccg atgaagtagt cgttcaccca ggtccggtcg gccatggtca cgcgaccgag
     2341 gtagcatgcc aggtcccggc cccggagggc ttccggaagg gtgaactcga tcctgagcca
     2401 tacgaacccc gagcccgagg ggaggagccg ttctagattc gtgaacgagg cggagtccag
     2461 cggcgcgaaa tcgcctacct tgccgccctc gtcctcgatc gcccactgcc agcccgtatc
     2521 cagcgagagc tcgagcgcat cgtcgagagc ggctccccag atgctgtacg cgccgagtat
     2581 tgccgctatg gctacgccta gaacggtatt cctcgttacc atgactctag cgcgctccgc
     2641 cctcgccctt atccaggctg gccatgacct tcttctcgtt ataggcgagg aggaggacga
     2701 gcccgagcgc gcagaagccc agggcgaaga aggtggtcat gcgcacgccg ctcttgccca
     2761 cgatctcccc ggccgcctcg tcggcgccga tcatgacgat ggacggcagg atcagggctc
     2821 cgacgaactg gcccagtttc gacatgaagg tcctgaagcc gaagaagacg ccggccttat
     2881 ggacgccggt ctcgatcgag taggcttcgg ccatgtcgga tatcatggca ttggggagga
     2941 tgccgaagat agccatcgga atagaggcga ggatggccgc gatggcagcc tgggccatgg
     3001 ccggcagggg caggacgccg aagagcgagc agaagccgaa cagcacgccg aagatgacga
     3061 acgcgacgat gaggagccgc ctcctgccta ccttcctggc cagcaggcct acgggcacgt
     3121 agaagatgaa gctcacggcg aacatgacga tgaagagcag ggagtaagtc tccttcggga
     3181 gcccgaggag cacggtgatg taatagagga ggccgttgtt gataaaatag aggctgaccc
     3241 agtatgcgaa gtccgagagg gtgaagacaa ggaagttgcg gtccttgatc accttgccga
     3301 cggacgagaa gaagccgtcg tcgctcggta tggagacgca gtatttcttc tcgtcgatga
     3361 agacgacggg cagcatcat
//
LOCUS       DAOPHV010000263         3378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_38879_length_3378_cov_3.395125, whole genome shotgun sequence.
ACCESSION   DAOPHV010000263 DAOPHV010000000
VERSION     DAOPHV010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38879_length_3378_cov_3.395125"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PLQ29_07660"
     CDS             complement(<1..917)
                     /locus_tag="PLQ29_07660"
                     /inference="COORDINATES: protein motif:HMM:NF022635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HPG86560.1"
                     /translation="MANLLDYLRWRGDLTLSASPFNSVDSLLLSVLAYVPFDGVVPSS
                     VTKKGPTIAEAATTLLETGAAGPAFRDENDLLLLSALASSERFGNMRLCAYESVLDPE
                     REQQFAAVTAMTGDGHAFVAYRGTDHTLVGWKEDFNMSFMSPVPAQEAAVAYLEHVAS
                     GLGLGPRLRLGGHSKGGNLAVYAASFCRARTQRRVRAVYNNDGPGFDATVIAREGYRN
                     VQERIRSFVPQSSIIGMLLEHHERYTVVQSTESGLMQHDPYSWSVLSTDFITVKQVTD
                     ESLFVDRALKEWVGAMAPAQRELFIDAVF"
     gene            complement(925..1272)
                     /locus_tag="PLQ29_07665"
     CDS             complement(925..1272)
                     /locus_tag="PLQ29_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HPG86561.1"
                     /translation="MALQVFGTRKCAITRKAERFFKERDVPYHFVDLAEKGLSAGELD
                     AVAGAIGLASLIDEGSKRFKDRGLAYMDYDPREEILGNPALLKTPIVRDGRLAAVGDD
                     PPAWADFAKRSKG"
     gene            1357..2031
                     /locus_tag="PLQ29_07670"
     CDS             1357..2031
                     /locus_tag="PLQ29_07670"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPG86562.1"
                     /translation="MALHGSGRIISTIKDQVYSVIKENILNENLKPGQRIQELQIAKE
                     LNVSRSPVRSAINELIGEGLLESIPNKSVTVRRMSEKDILDAYEFRLVVEGFAVQRVI
                     ELMDDDIAATLARFREQFTATVDYGQIQEYVRIDTAFHEYLVNTAGNTIFKEALGRVA
                     MMITPFRIFSLSSQQRFLDSIREHTGLIDAILGRDADRASDICNAHLRLAKEEIIKHL
                     RSSIIA"
     gene            2251..>3378
                     /gene="larA"
                     /locus_tag="PLQ29_07675"
     CDS             2251..>3378
                     /gene="larA"
                     /locus_tag="PLQ29_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HPG86563.1"
                     /translation="MHIALPYDKGRIELEVADDRVAGVLTVPRHAAGGRSEVEIVRAA
                     HRAPLGSKPLSELAVGKKRVVVITSDHTRAVPSALTLPILLEEIRKGSPDAEITVLVA
                     TGLHRGMTPEEMESKFGKDVCARERIVNHDAYGTGRFVDLGVLPSGSKCEINRMAVEA
                     DLLVAEGFIEPHFFAGFSGGRKSVLPGIASQDCVNINHSAYAIQHPRAATGVLDGNPI
                     HEDMIEAARLAKLAFVFNVLLDEEKRVIAAFCGEADAAHRAGCEFLLARSGVDPVKAD
                     IVVTTNGGYPLDQNLYQCPKGLDAALACVNDGGVIILAAACRDGLGGDNFGRMMLEGT
                     PEELLERILATAPDKTISEQWCVQRFADALIKYRIILVTTGL"
BASE COUNT          629 a         1073 c         1078 g          598 t
ORIGIN      
        1 tcgaagacgg cgtcgatgaa cagctcgcgt tgggccgggg ccatggcgcc gacccattcc
       61 ttgagggctc ggtcgacgaa caggctctcg tccgtgacct gcttgacggt gatgaaatcg
      121 gtgctgagta cgctccagga gtacggatcg tgctgcatga ggccgctctc ggtgctctgg
      181 acgaccgtgt agcgctcgtg gtgctcgagc agcatgccga tgatcgacga ctgcgggacg
      241 aaggagcgaa tgcgctcctg tacgttccgg tagccttccc gggcgatcac cgtggcgtcg
      301 aagccggggc cgtcgttgtt atagacggcc cggacgcgac gctgggtccg ggcgcggcag
      361 aacgacgcgg cgtagacagc taggttgccg cccttcgagt ggccgccgag cctcagcctg
      421 ggcccgagtc cgagcccgga ggcgacgtgc tccaggtacg cgacggccgc ctcttgagcc
      481 ggtaccgggc tcatgaagct catattgaag tcctctttcc agccgacgag ggtgtggtcg
      541 gtcccgcggt aggcgacgaa ggcgtggccg tccccggtca tcgccgtgac cgcggcgaac
      601 tgctgttcac gctcggggtc gaggacgctc tcgtaagcgc ataggcgcat attcccgaag
      661 cgttccgagg aggccagggc ggacagcagc aggaggtcgt tctcgtccct gaaggccggg
      721 cccgcggcgc ccgtctcgag gagcgtcgtc gccgcctcgg ctatcgtcgg ccccttcttg
      781 gtgacggacg atggcacgac gccgtcgaag ggtacgtagg cgaggacgct gagtagtaag
      841 ctatccactg aattgaatgg gctcgcgctc agcgtaaggt cgccgcgcca gcgcaggtag
      901 tcgagcaggt tcgccatggt cgtcctagcc cttcgagcgc ttggcgaagt cagcccacgc
      961 gggaggatcg tcgcctaccg cggcgagcct gccgtcacgc actatcggcg tcttgagcag
     1021 ggcggggttc cccaggatct cctcgcgggg atcgtagtcc atgtacgcca gtcccctatc
     1081 cttgaagcgt ttcgagccct cgtctatgag cgaagcgagg ccgatcgccc cggcgacggc
     1141 gtcgagctcg cccgcgctga ggcctttctc cgccagatcg acgaaatggt acggtacgtc
     1201 gcgctccttg aagaagcgct cagccttgcg cgttatagcg cacttgcggg ttccgaaaac
     1261 ttgcagagcc atgctcaacc tccagcggag ccgtcgtcga atcgggtcac gaagccgttc
     1321 attgccaagt ataccatttt tctgtattct agcggcatgg cattgcatgg tagcgggcgg
     1381 atcatttcga cgatcaagga tcaggtgtac tccgtcatca aggagaatat cctcaacgag
     1441 aacctgaagc ccgggcagcg catccaggaa ctccagatag ccaaggaact gaacgtcagc
     1501 cgctctcccg tgcgcagcgc catcaacgag ctcatcggcg aaggcctgct cgagtccatt
     1561 cccaataaga gcgtaacggt gcgcaggatg tcggagaagg atatcctcga cgcgtacgag
     1621 ttcaggctcg tcgtcgaggg cttcgccgta cagcgggtga tcgagctcat ggacgacgat
     1681 atagcggcca ctctggcccg tttcagggag cagtttacgg cgaccgtcga ctatggccag
     1741 atccaggaat acgtccggat cgatacggcc ttccacgagt acctcgtcaa tacggcgggt
     1801 aacacgatct tcaaggaagc cctcggtcgc gtcgcgatga tgatcactcc tttcaggata
     1861 ttctcgttgt cgagccaaca gcgcttcctc gactcgatac gggagcacac cggcctcatc
     1921 gacgccatac tcggacgcga cgccgatagg gcttcggata tctgcaacgc ccacctgcgc
     1981 ctggctaagg aagagatcat caagcacctg cgttcatcga tcatcgcgta gccagcctcc
     2041 gtatagttcg agacgctcgt catcctcccg tagcgtcgcc gcgccagggc gacggccctg
     2101 ggcgaggcgc gcccacctca tgccaatcca tcccagcggc tacccctcac tcggttaacg
     2161 gttgacaagg tataaagaca gtgtattatg tatacataat acatttcatg ctgttatccc
     2221 atacgaacga actgtcgcga aggagtcgct atgcatatag ccttgcccta tgacaagggc
     2281 cgtatcgaac tggaggtcgc cgacgatagg gtcgccggcg tgctgaccgt gccgcggcat
     2341 gcggccggcg ggcggagcga ggtggagatc gttcgggccg cgcatcgcgc tcccctgggc
     2401 tcgaagccgc tgtccgagct ggccgtcggg aaaaagcgcg tcgtcgtcat cacgagcgac
     2461 catacgcgcg ctgtgccgag cgccctgacc ctgcccatcc tgctcgagga gatacgcaag
     2521 ggtagcccgg acgcggagat caccgtgctc gtcgccaccg gcctccaccg aggcatgacg
     2581 cccgaggaga tggaatccaa gttcggcaag gacgtctgcg cccgggagcg tatcgtcaac
     2641 cacgacgcgt acggcacggg ccggttcgtc gacctcggcg tgctgccgtc aggaagcaag
     2701 tgcgagatta accggatggc cgtcgaggcc gacctgctcg tcgccgaggg cttcatcgag
     2761 ccgcacttct tcgccggctt ctcaggcgga cgcaagagcg tgctgcctgg gatagcgtcg
     2821 caggactgcg tcaacatcaa ccatagcgcg tacgccatcc agcatccgcg cgcggccacc
     2881 ggcgtgctcg acggcaaccc catccacgag gacatgatag aggcggccag gctggcaaag
     2941 ctcgccttcg tattcaacgt gctcctcgac gaggagaagc gcgtgatcgc cgccttctgc
     3001 ggcgaggccg acgcggcgca tcgggccggc tgcgagttcc tgctcgcccg gagcggcgtc
     3061 gacccggtca aagccgatat cgtcgtcacg acgaacggcg gctacccgct cgaccagaac
     3121 ctgtaccagt gccccaaggg cctggacgcg gcgctggcct gcgtcaacga cggcggcgtg
     3181 atcatcctgg cggccgcctg ccgggacggc ctcggcggcg ataatttcgg gcgcatgatg
     3241 ctcgagggca cgcccgagga actgctcgag cgcatcctcg cgacggcgcc cgataagacg
     3301 atatcggaac agtggtgcgt gcagcgcttc gcggacgcgc tcatcaagta caggatcata
     3361 ctcgtcacga ccggccta
//
LOCUS       DAOPHV010000264         3361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_39153_length_3361_cov_3.091047, whole genome shotgun sequence.
ACCESSION   DAOPHV010000264 DAOPHV010000000
VERSION     DAOPHV010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39153_length_3361_cov_3.091047"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..800)
                     /gene="lptC"
                     /locus_tag="PLQ29_07680"
     CDS             complement(141..800)
                     /gene="lptC"
                     /locus_tag="PLQ29_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPG86564.1"
                     /translation="MPRAGRGTERLSSRARSMAAAFVAAQVALVALVGCSLDYGDGLA
                     NDLSEDIPDTVVHGFSHTVVENGLPSFRIEADRAESYQGKRLMVLSGVRFREYGRDGV
                     ELLSEGGAEHASFHTDTESAELSGSVRFYYAPDGLTVESGYLEWDGEARRLSSRADTV
                     TTLRDDDGSGLTGSGFAADAASRSFEFGNRADGRFVAVDDVVAVDDVVAEYDSGEEPD
                     R"
     gene            complement(793..2412)
                     /locus_tag="PLQ29_07685"
     CDS             complement(793..2412)
                     /locus_tag="PLQ29_07685"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPG86565.1"
                     /translation="MKKLIFVTGGVCSSLGKGVTASSIGSLLEARGLAVRMLKIDPYL
                     NVDAGTMNPYQHGEVYVTDDGAETDLDLGNYARFTNAPISRANSITTGQVYREVINKE
                     REGRFLGKTVQVVPHITDEIKARVVAAGKDPEADVTIVEVGGTVGDMESVPFLEAARQ
                     LIHEYGRQNAVSVHVTLVPEVTGGELKTKPTQHSVKELQEVGIQPDVLVCRSVSGLDE
                     SLRRKLAAFTNVEYEGVISAKDASTIYQIPLVFHEQGLDQFLLAKMNVESRHADLRAW
                     KEVVRKLTEPRRRVRIGVVGKYMELHDAYKSVWEALYHGGIGNDAGVEIVRIDSSRFE
                     VEGADPGAILSDVDGLLVPGGFGQRGIDGMVAAARWAREHGLPYFGLCLGMQIMAIEW
                     ARDVLGWIDANSAEFAPDAAKAVVSLLEDQVDVKNYGGTMRLGSNDSVFKDGSKPALA
                     YGSTRVSERHRHRYEFSNAFRGDFEASGLVIAGTTPDGELVECLEWPGHPWGVGVQYH
                     PEFKSKPTAPHPLFTAFVSAALEYGKKKAHA"
     gene            complement(2466..2915)
                     /locus_tag="PLQ29_07690"
     CDS             complement(2466..2915)
                     /locus_tag="PLQ29_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPG86566.1"
                     /translation="MALIDLIEESVVRVPLAAVGKDAAIRELVDTLVSAGKILDAETA
                     YRAVLDREAKGSTALEEGIAVPHGKTLAARELTIAIGISAAGVEFGSMDGKPTRLFFL
                     LLAPPDKAGPHIEALAEIARMSRSKAFCASLAACRTPSEVVRLLKDE"
     gene            complement(2926..3360)
                     /locus_tag="PLQ29_07695"
     CDS             complement(2926..3360)
                     /locus_tag="PLQ29_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPG86567.1"
                     /translation="MTAAQYAIVLAKTLGADLYALYVVDTRALAELVNSRIFLESEQE
                     EYRRDLDGDASRYLNHVRELARRKGVPVAAEKASGAVHVEIKAKVASLGVDLLVLGEI
                     SQIRSRRDELYNETERAMRTAQCPVLVAKGEDKIWDLYESLE"
BASE COUNT          536 a         1136 c         1107 g          582 t
ORIGIN      
        1 gttgccgacg agcaccgccc gctcgcgtcc cttggccatg gccccggaca tcgagtcggc
       61 tgagaaggag aagtcgtcgg ccgcgacgga tgccgcggct agggccaagg ccagggcggc
      121 gacgaagaag cgccggcggc tcatcggtcg ggttcctctc ccgagtcgta ctcggcgacg
      181 acgtcatcga cggcgacgac gtcatcgacg gcgacgaacc ttccgtcggc gcggttaccg
      241 aactcgaagg accggctggc agcgtccgcc gcgaagccgg aacctgtcag gccggaacca
      301 tcatcgtcgc gcagcgtcgt gacggtatcc gcgcggctcg acaggcgcct agcctcgccg
      361 tcccactcga ggtagcccga ttcgacagtc aagccgtcgg gggcatagta gaaacgcacc
      421 gaacccgaca gctcggcgct ctcggtatcg gtatggaacg aggcgtgctc ggccccgccc
      481 tccgaaagca gctcgacgcc gtcccggccg tactctcgga agcgcacgcc cgacagcacc
      541 atcaggcgtt tgccctggta cgactcggcc cggtccgctt cgatgcggaa gctcgggagg
      601 ccgttctcga cgacggtatg ggaaaaaccg tggacgaccg tatccggaat atcctccgac
      661 aggtcgttcg ccaagccgtc gccgtaatcg aggctacagc ctactagcgc gaccagcgcg
      721 acctgcgccg cgacgaacgc ggcggccatc gagcgcgcgc gggaggaaag ccgctcggtt
      781 ccgcggccgg ccctaggcat gcgccttctt cttgccatac tcgagcgccg ccgagacgaa
      841 ggcggtgaat agcgggtgcg gggcggtcgg cttcgacttg aactccggat gatactgcac
      901 gccgacgccc caggggtggc cgggccactc gaggcactcg accagctcgc cgtccggagt
      961 cgtgccggcg atcacgaggc ccgacgcctc gaagtcgccg cggaaggcgt tcgagaactc
     1021 gtagcgatgg cggtggcgct ctgagaccct ggtcgagccg taggccagcg ctggcttgga
     1081 gccgtccttg aagaccgagt cgttgctgcc gagacgcatc gtgccgccat agttcttcac
     1141 gtcgacctgg tcctcgagca ggctgacgac cgccttggcc gcgtcgggcg cgaattccgc
     1201 cgagttggcg tcgatccagc cgagcacgtc cctggcccac tcgatggcca tgatctgcat
     1261 gccgaggcac agtccgaagt acggcaggcc gtgttccctg gcccacctgg ccgcggccac
     1321 catgccgtct atgccgcgct ggccgaagcc gcccggcacc aggagcccgt ccacgtcgga
     1381 caggatcgcg cccgggtcgg cgccctcgac ctcgaagcgg ctcgagtcta tcctgacgat
     1441 ctcgacgccc gcgtcgttgc ctatgcctcc gtggtacagg gcctcccaga cggacttgta
     1501 ggcgtcgtgc agctccatgt acttgccgac gacgccgatg cgcacgcgcc gtctgggctc
     1561 ggtcagcttg cggaccactt ccttccaggc gcggaggtcg gcgtggcggc tctccacgtt
     1621 catcttggcc agcaggaact gatccaggcc ctgctcgtgg aacacgagcg ggatctggta
     1681 gatcgtcgag gcgtccttgg cggagatcac gccctcgtac tcgacgttcg tgaacgccgc
     1741 gagcttccgc ctgagcgatt cgtcaaggcc ggagacggaa cggcagacga ggacgtcggg
     1801 ctggatgccg acctcctgca gctccttgac ggagtgctgg gtcggcttgg tcttgagctc
     1861 cccgcccgta acctcgggca ccagggtgac gtggacgctg acggcgttct gcctaccgta
     1921 ctcgtggatc agctggcggg ccgcttcgag gaagggtacg ctctccatat cgccgacggt
     1981 gccgcccacc tcgacgatgg tcacgtcggc ctccgggtcc ttgcccgcgg cgacgactcg
     2041 ggccttgatc tcgtcggtga tatgcggtac gacctgcacc gtcttgccga ggaagcggcc
     2101 ctcgcgctcc ttgttgatca cctctcggta gacctgcccg gtcgtgatcg agttggccct
     2161 ggagatgggc gcgttcgtga acctggcgta gttgccgagg tccaggtcgg tctccgcgcc
     2221 gtcgtcggtc acgtagacct cgccgtgctg atacgggttc atcgtaccgg cgtccacgtt
     2281 caagtaggga tcgatcttga gcatgcgaac cgctagcccg cgcgcctcga ggaggctacc
     2341 gatcgatgag gcggttacgc ccttgccgag tgaggagcat acgccgcccg tcacgaagat
     2401 aagcttcttc atggaaggcc attatcggcc gtcggccgcg gagggtcaag cgcccaggta
     2461 cgcgactact cgtccttgag gaggcgcacc acctcggaag gcgtgcggca ggcggcgagc
     2521 gaggcgcaga acgccttgga cctggacatc ctggctatct cggcgagcgc ctcgatgtgc
     2581 ggcccggcct tatccggcgg cgccagcagc aggaagaaca gcctggtcgg cttaccgtcc
     2641 atcgagccga actcgacgcc ggcggccgag atcccaatgg ctatcgtcag ctcgcgggcg
     2701 gcgagcgtct tgccgtgggg tacggctatg ccctcctcga gcgcggtcga gcctttggcc
     2761 tcgcgatcga gcacggctcg gtaggcggtc tcggcatcga gtatcttgcc ggccgagacg
     2821 agcgtgtcga ccaattccct gatggccgcg tccttgccga cggcggcgag gggaaccctg
     2881 acgacgcttt cttctatcaa gtcgatcagt gccatgtcag ccttcctatt ccaaggactc
     2941 gtacaggtcc catatcttgt cttcgccctt ggcgacgagc accgggcatt gagcggtccg
     3001 catggcccgc tccgtctcgt tgtagagttc gtcgcgacgc gaccgtatct gggatatctc
     3061 gccgagcacg agcaggtcga cgccgaggga cgccaccttg gccttgatct cgacatgcac
     3121 cgcgcccgag gccttctccg ccgccaccgg cacgcccttg cgcctggcca gttctcgcac
     3181 gtggttcaga tagcgcgagg cgtcgccatc gaggtcgcgc ctgtactcct cctgctccga
     3241 ctcgaggaag atccgcgagt tgacgagctc ggcgagggcc ctcgtatcga cgacgtagag
     3301 cgcatatagg tcggcgccga gcgtcttggc cagcacgatg gcgtactggg ccgccgtgat
     3361 c
//
LOCUS       DAOPHV010000265         3335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_39566_length_3335_cov_3.878963, whole genome shotgun sequence.
ACCESSION   DAOPHV010000265 DAOPHV010000000
VERSION     DAOPHV010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39566_length_3335_cov_3.878963"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..827
                     /locus_tag="PLQ29_07700"
     CDS             189..827
                     /locus_tag="PLQ29_07700"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPG86568.1"
                     /translation="MIQALYVDDDFLIIDKPAGLATQPGEGVRTCVIDAVERDFGFRP
                     YLVHRLDKETAGCLVVARSPRAAAELSALMGGRGAVKAYRAVVAGRPEPADGVYRDDI
                     RVRGERVSAETRYRTLRGDDRLSLVEAELGTGRNHQIRLHFAQAGHPLAGDDRHGDFK
                     LNKALAKEYGIKRLMLYASRIWLPLVPPVGAEAPVPPHFAAFYERWPEGGAT"
     gene            824..1213
                     /locus_tag="PLQ29_07705"
     CDS             824..1213
                     /locus_tag="PLQ29_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86569.1"
                     /translation="MTAPFEWVRGAAWLFPDGTLDIVPGFHDEWIAAHQDRAPGCSNV
                     ADVVTRLGWVSVVSYSRGYVEFMIRSKSDSGSVRLCVEHLGRNLGRWENALVMTMDEE
                     GYAKLTPDDFSDGASPESKIRGIFSVD"
     gene            complement(1210..1983)
                     /locus_tag="PLQ29_07710"
     CDS             complement(1210..1983)
                     /locus_tag="PLQ29_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HPG86570.1"
                     /translation="MGDILVATSGYSYDDWRGAFYPEELPKSEFLRYYALFFPFVELN
                     FSYYAMPKASNLRSMAARTPSSFRFSIKAHRSLTHEPTASWRADAAEFAAAAGALAEG
                     DRLAAVLVQLPYSFHHTPDNRGYLAALLDALRPLPLVVEFRNDEWRGDRVYDELDRRG
                     VGVAMVDRPELPGLPAVEERVTGDLAYLRFHGRNADAWWSGDATSRYDYLYSSEELAA
                     SAPRLKRMARQGNLLVAFNNHARGNAVRNARELKELLGA"
     gene            complement(1992..>3335)
                     /locus_tag="PLQ29_07715"
     CDS             complement(1992..>3335)
                     /locus_tag="PLQ29_07715"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPG86571.1"
                     /translation="AIALAIGVAGAFGLSRIIIGPLMKVVRGIETIRDESDKRKLADF
                     SIDVRSRDELSILAGTINEMTAGLVEAAKEAEFLTVGKEVQKMFIPLVTNELGEKLTT
                     GYDDQAGHIFFGYYEGAKGVSGDYFDYTPLDGRFWAFIKCDVSGKGVPAALIMVGVAT
                     IFTTELQGWSFKRDGIHLDRITYKINDFIEKRGFKGRFAALIMGIYDSQTGAAYLCHA
                     GDNILRVYEAEKAAVVTHTLDSAPTAGTFPNDLVESTSPYKQVILPLSRGDTLLLYTD
                     GFEESSRAMRGPDFKPLYEVKKLRDREGNESEHREALVEQLGEERIKAVTEAIMARGS
                     YTLRKEGDPLGPDAAYDFDFSTLDAGPDDLVLGIAAVEKVFRIIPDPTAGADDRVIVD
                     ARIDEVLAKCWKQYARFCHDKQPHPDPRRKEYLYYGHLKEDEQYDDLTMMLIKRK"
BASE COUNT          496 a         1107 c         1153 g          579 t
ORIGIN      
        1 ttcccggcga tcatgggcgg ctgcgccgcg ctcgccctgt tcagcgcgtt cctgctccgc
       61 gaccggcgca agaagcccgg caaggccctg ctggcgccgg cggccggggc catcgtcgtg
      121 cacgccagga aatgagcgcg ccggcccggg cttgcgcccg ggccgagatg cgcgtatgct
      181 ttggcgccat gatccaggcc ctgtacgtcg acgacgactt cctcatcatc gataagcccg
      241 ccgggctcgc gactcagccg ggcgagggcg tgaggacctg cgtgatagac gcggtggagc
      301 gcgacttcgg cttcaggccc tacctcgtgc accgactgga caaggagacg gccggctgcc
      361 tcgtcgtggc gcgctcgccg cgggccgcgg ccgagctgtc ggcgctgatg ggcggccggg
      421 gagccgtgaa ggcgtaccgc gccgtcgtcg ccggcaggcc cgagccggcc gacggcgtct
      481 accgggacga tatacgggtc cgcggcgagc gcgtgagcgc cgagacccgc taccgcacgc
      541 tccgcggcga cgaccggctg agcctcgtcg aggccgagct gggcaccggg cgcaaccacc
      601 agatacggct gcacttcgcc caggcgggcc acccgctcgc cggcgacgac cgccacggcg
      661 acttcaagct gaacaaggcc ttggccaagg aatacgggat caagcgcctg atgctctacg
      721 cctcgaggat atggctgccg ctcgtccccc cggtcggggc ggaggcgccc gtgccgcccc
      781 acttcgccgc gttctacgag cgctggccag aaggaggcgc gacgtgaccg cgccgttcga
      841 atgggtccgc ggcgccgcct ggctgtttcc cgacggcacg ctcgacatcg tccccggctt
      901 tcacgacgaa tggatagcgg cccatcagga ccgggcgccc ggttgctcga acgtggcgga
      961 cgtggtgacc aggctcggtt gggtgtcggt ggtctcgtac tcgcgtggct acgtcgagtt
     1021 catgatacgc tccaagtcgg actcaggctc ggtcaggcta tgcgtcgagc acctcgggcg
     1081 taacctgggc cgctgggaga atgccctcgt gatgacgatg gacgaagagg gctacgccaa
     1141 gttgactccg gacgacttct ccgacggcgc ctcgcccgag tcgaagatcc gcggcatctt
     1201 ctcggtcgac taggcgccga gcagctcctt gagctcgcgc gcgttgcgga cggcgttacc
     1261 ccgcgcgtgg ttgttgaacg ccacgagcag gttcccctgc ctcgccatcc gcttgagcct
     1321 cggcgccgaa gccgccagct cctcgctgga gtacaggtag tcgtagcggc tcgtcgcgtc
     1381 gccgctccac caggcgtccg cgttacggcc gtggaagcgc aggtaggcca ggtcgccggt
     1441 cacgcgctcc tcgaccgccg gcaggccggg cagctccggg cggtcgacca tcgcgacgcc
     1501 gaccccgcgc cggtcgagct cgtcgtagac gcggtcgccc cgccactcgt cgttgcggaa
     1561 ctcgacgacg agcggcagcg gcctgagcgc gtcgaggagg gcggccaggt agccgcggtt
     1621 gtccggcgtg tggtggaagc tgtacggtag ctgcaccagc acggcggcga gcctgtcacc
     1681 ctcggccagc gcgcccgcag ccgccgcgaa ttcggccgcg tcggccctcc acgaggcggt
     1741 cggctcatgc gtgaggctcc tgtgcgcctt gatgctgaat cggaagctcg aaggcgtcct
     1801 agcggccatc gagcgcaggt tcgacgcctt gggcatggcg tagtacgaga agttgagctc
     1861 gacgaacggg aagaaaaggg cgtagtagcg caggaactcc gacttgggca gctcctcggg
     1921 gtaaaaggct ccgcgccaat cgtcgtagga gtagccgctc gtggccacca ggatatcgcc
     1981 catgccccgg ctcacttccg cttgatcagc atcatcgtca ggtcgtcgta ctgctcgtcc
     2041 tccttgaggt ggccgtagta caggtattcc ttgcgcctcg ggtcgggatg gggctgcttg
     2101 tcgtggcaga acctggcgta ttgcttccag cacttggcca gcacctcgtc gatacgggcg
     2161 tcgacgatga ccctgtcatc ggccccggcg gtcgggtcgg ggatgatcct gaagaccttt
     2221 tccacggccg cgatgccgag cacgaggtcg tccgggccgg cgtcgagggt cgagaagtcg
     2281 aaatcgtagg cggcgtcggg gccgagcgga tcgccctcct tgcgcagcgt gtacgagccc
     2341 cgcgccatga tggcctcggt gacggccttg atgcgctcct cgccgagctg ctccaccaga
     2401 gcctcccggt gctcgctttc gttgccctcg cggtcgcgca gcttcttgac ttcgtagagc
     2461 ggcttgaagt ccggtccgcg catcgcgcgg ctcgattcct cgaagccgtc cgtgtagagc
     2521 agcagggtgt cgccgcggct caggggcagg atcacctgct tgtacggcga ggtggactcg
     2581 acgaggtcgt tcgggaaggt gccggcggtc ggcgcgctgt cgagggtatg ggtgacgacc
     2641 gccgccttct cggcctcgta gacgcgcagg atgttgtcgc cggcgtggca gagataggcg
     2701 gctccggtct gcgagtcgta gatgcccatg atcaaggccg cgaatcggcc cttgaagccg
     2761 cgcttctcga tgaagtcgtt gatcttgtag gtgatgcggt ccaggtggat gccgtcgcgc
     2821 ttgaacgacc agccctgcag ctcggtcgtg aagatcgtcg ccacgccgac catgatgagg
     2881 gcggccggca cgcccttgcc ggatacgtcg cacttgatga aggcccagaa tcggccgtcg
     2941 agcggggtgt agtcgaagta gtctcccgat acgcccttgg cgccctcgta gtagccgaag
     3001 aagatgtggc cggcctggtc gtcgtagccg gtcgtcagtt tctccccgag ctcgttcgtg
     3061 acgaggggga tgaacatttt ctggacttcc tttcctacgg tcaggaactc cgcttccttg
     3121 gccgcctcga cgaggccggc ggtcatctcg ttgatggtgc cagccaggat cgacagctcg
     3181 tcgcggctcc tgacgtcgat ggagaaatcc gccagcttgc gcttgtcgct ctcgtccctg
     3241 atggtctcga tgcccctgac caccttcatg agcgggccga tgatgatgcg cgacaggccg
     3301 aaagctccgg ccacgccgat ggccaacgct atcgc
//
LOCUS       DAOPHV010000266         3332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_39616_length_3332_cov_4.206286, whole genome shotgun sequence.
ACCESSION   DAOPHV010000266 DAOPHV010000000
VERSION     DAOPHV010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39616_length_3332_cov_4.206286"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..656
                     /locus_tag="PLQ29_07720"
     CDS             75..656
                     /locus_tag="PLQ29_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86572.1"
                     /translation="MDRRNTRTYNCIFALAVVMLALGTGSLAVTTKGIAAERVAPPLA
                     FFAVGSIVSYFAVIGRWKAKYVFIGMMLGLGALIRFVFIMLGVSPIDYWPLFAVAAGV
                     ALMPANYARRGRIRPSSIVISSSFVLMGLFLSIFSFGFSSTSFKTFIARWWPLLFIAS
                     GVVLLIAWGIQRLLSRAEERGEARGASPGGGEP"
     gene            653..2344
                     /locus_tag="PLQ29_07725"
     CDS             653..2344
                     /locus_tag="PLQ29_07725"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG86573.1"
                     /translation="MSRAAAARLALAVALASASCVGGPEPRSGTSLSSGTGQGAIQGE
                     RAAVEAAIALGSPSSLELAVELAEAASAIPQADARAYGWVAYEMARLVYPELAGGLPP
                     SSMAPPDSPLVRAFIDARNGKQVAPSAAAGPLFELFPALAVFRIKTTDSAGATLAAVE
                     RFNRFGLPSAAAELARGIALERSGDLQGAYSAYARAEEAANDCYPATLGGARMLVGLG
                     RGEEALEVASRLDARIVESVAGRRVKAMALYQAKRWAEALPLITAVLLDDPLDSRFAL
                     MRAHLLIERGEYKQAAPLLDAYASINPDDRLYILLRARIAMESAKDRSQASAALRRGL
                     TRYPSDPELTLYAAELFAGGDPAERAEALALAQKAYDADPSSIRALRLLLGFDLAAGD
                     YTAAAAKADAVLASGMAFGDTEALYRSYRGAGRSVDALALARSWRAKEPGSEAAAVAL
                     ATSMVDLGQKAAASELIGTLLASKGTPAYRSTLYWLQSRLQPGDEAALSSLRSALVEN
                     GMNIDALAAMSDIYVRRGDYQRARFYLKQAIAISPDRADIVERRNVLAQLGVAIP"
     gene            2386..3168
                     /locus_tag="PLQ29_07730"
     CDS             2386..3168
                     /locus_tag="PLQ29_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HPG86574.1"
                     /translation="MARRLDALYDEYNDPRWIEPDPLAVVRRYDDPADVELAGLAAAA
                     LALGNAALIVRAAEAALAPLGPRPASALAEMDDEDLAASLASFRYRFFDGGDLASLLA
                     AARNLRRRHGGLEAAFLAGDDPESGDYVGAVSRFVSLLASAAPAPWRPNLLPDPARGS
                     AAKRAFLYLRWMVRRDSVDPGPWSRCDPSRLVAPLDTHMAAACRRLGLLSRRGTDLKA
                     AVEATSRLRAIRPDDPIRYDFCMTRPGIRPDLDPDACFEPFH"
     gene            3248..3324
                     /locus_tag="PLQ29_07735"
     tRNA            3248..3324
                     /locus_tag="PLQ29_07735"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3282..3284,aa:Pro,seq:ggg)
BASE COUNT          454 a         1208 c         1120 g          550 t
ORIGIN      
        1 actataccac gcgctctttt gcgttggcaa ggaatcgctt tgtggataca ctagagacgg
       61 aggctagagt aggcatggat cggcggaata cccggacgta taattgcatt ttcgccctcg
      121 ccgtcgtgat gctggcgctc gggaccggct cgctggcggt gacgaccaag gggatagccg
      181 ccgagcgcgt ggccccgccg ctggccttct tcgccgtcgg atccatcgtc tcctacttcg
      241 ccgtgatcgg ccgctggaag gccaaatacg tattcatcgg catgatgctg ggcctcggcg
      301 cgctgatacg cttcgtcttc atcatgctcg gcgtcagccc catcgactac tggcccctgt
      361 tcgccgtggc ggccggtgtg gccctgatgc cggcgaacta cgcgcggcgt gggcgcatta
      421 ggccgagttc catcgtcatc tcgtcgtcgt tcgtactcat gggcctgttc ctgtccatct
      481 tctccttcgg attcagctcg acgagcttca agaccttcat agcccggtgg tggcccctcc
      541 tattcatcgc ctccggcgtc gtgctgctga tagcctgggg tatccaacgc ctgctatcgc
      601 gcgccgagga gcgcggcgag gcgcgcggcg cttccccggg cggaggcgag ccatgagtcg
      661 agccgcggcc gcgcgcctcg cgctggccgt cgcgctagcc tccgcctcct gcgtcggcgg
      721 gcccgagccc cgctccggaa cgtcgctgtc gtccgggacc ggccaaggcg cgatccaggg
      781 cgagcgcgcc gccgtcgagg ccgccatagc gctcggtagc ccgtccagcc tggagctggc
      841 cgttgagctg gccgaggcgg cgtcggcgat accccaggcc gacgcgaggg cctacggctg
      901 ggtggcctac gaaatggcgc gcctcgtgta tccggagctg gccggcggcc tgcccccgag
      961 ctcgatggcg cctcccgact cgccgctcgt gcgcgcgttc atcgacgcca ggaacggcaa
     1021 gcaggtagcg ccgtcggccg ccgccggtcc cctgttcgag ctgttccccg cgctggcggt
     1081 ctttaggatc aagacgaccg attcggccgg cgcgacgcta gccgccgtcg agcgtttcaa
     1141 ccgcttcggc ctgccctcgg ccgccgccga gctggcccgc gggatagccc tggagcgatc
     1201 gggcgacctg cagggcgcct actcggccta tgcccgggcg gaggaagcgg ccaacgattg
     1261 ctacccggcc acactgggcg gcgcccggat gctcgtcggg ctgggccgcg gcgaggaggc
     1321 gctcgaggtc gcgtccaggc tcgacgcgag gatagtcgag tcggtcgccg gccgtcgcgt
     1381 caaggctatg gccctctacc aggccaagcg ctgggccgag gccttgccgc tgatcaccgc
     1441 ggtgctgctc gacgaccccc tcgactcgcg cttcgccctg atgcgcgcgc accttctgat
     1501 cgaacggggt gagtacaagc aggccgcgcc gttgctcgac gcatatgcga gcatcaaccc
     1561 cgacgatcgc ctctatatac tgctccgcgc gcgcatagcg atggagagcg ccaaggaccg
     1621 gagtcaggcc tcggccgccc tgcgccgggg gctgacgcgc tatccgtccg acccggaact
     1681 gactctctac gctgcggagc tgttcgccgg cggagacccg gccgaacggg ccgaggccct
     1741 ggccctggcc cagaaggcct acgacgcgga tccgtcgtcg attcgggcgc tccggctgct
     1801 cctgggcttc gacctggcgg ccggggatta tacggccgcc gcggccaagg ccgacgccgt
     1861 actggcctcc gggatggcct tcggcgatac cgaggcgcta taccgctcct accgcggcgc
     1921 cggtcgctcg gtcgacgcgc tggccctggc ccgctcgtgg cgcgcgaagg aacccggctc
     1981 cgaggcggcg gccgtcgcgc tggccacctc gatggtcgac ctcggccaga aggcggccgc
     2041 gtccgagctg atagggacgc tcctggcctc gaagggtacg ccagcctacc ggtcgaccct
     2101 gtactggctc cagtccaggc tccagcccgg cgacgaggcg gcgttgtcgt ccctacgctc
     2161 ggccctggtc gagaacggca tgaacatcga cgccctggcc gccatgtcgg acatctacgt
     2221 gcgcaggggc gactaccagc gtgcgcgctt ctacctgaag caggcgatag ccatatcccc
     2281 ggaccgagcc gacatcgtcg agcggcgcaa cgtgctggcc cagctgggcg tggcgatacc
     2341 ctgagcgccg agcctatccg ggcctcgggc tgtcccgacg cgacattggc ccgccgtctc
     2401 gacgccctgt acgacgagta caacgacccc cgatggatag aacccgaccc gctcgccgta
     2461 gtcaggcgct acgacgaccc cgcggacgtc gagctggccg gcctcgcggc ggcggccctg
     2521 gcgctcggca acgccgcgtt gatcgtccgg gcggccgagg cggcgctggc gcccctcggg
     2581 ccgcgtccgg cgagcgccct ggccgagatg gacgatgaag acctggcggc ctcgctggcg
     2641 agcttccgct accgcttctt cgacggcgga gacctggcct cgctgctggc ggccgccagg
     2701 aacctacggc ggcgccacgg cggcctggaa gcggccttcc tggccggcga tgacccggag
     2761 agcggcgact acgtcggggc tgtctcccgc ttcgtctcgc tgctcgcctc ggcggccccg
     2821 gcgccctggc ggccgaacct gttacccgat ccggctcgcg gctcggcggc caagcgcgcg
     2881 ttcctctacc tccgctggat ggtccgccgc gactccgtcg accccggccc ctggagccgt
     2941 tgcgaccctt ccagactggt cgcgccgctc gatacccaca tggccgccgc ctgtaggcgc
     3001 ctcggcctcc tgtcccgccg cggcaccgac ctcaaggcgg ccgtcgaggc gacctctcga
     3061 ctcagggcga tacggccgga cgacccgata cgctacgact tctgcatgac caggccgggt
     3121 atccgtcccg acctggaccc ggacgcctgc ttcgagccgt ttcattgaca cgccttcggc
     3181 cagcgtgcta gtatcctcgg gcaccgacgc gaccgctcgg cgcgatatcg ggtatagttt
     3241 ttttggtcgg gcaatagcgc agttggttag cgtactagtc tgggggacta ggggtccccg
     3301 gttcaaatcc gggttgcccg accaaaaaat ct
//
LOCUS       DAOPHV010000267         3322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_39794_length_3322_cov_4.147536, whole genome shotgun sequence.
ACCESSION   DAOPHV010000267 DAOPHV010000000
VERSION     DAOPHV010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39794_length_3322_cov_4.147536"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLQ29_07740"
     CDS             <1..374
                     /locus_tag="PLQ29_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPG86575.1"
                     /translation="NKDNVDLDGYDNIPRQRAAHAPAKDAFKDVRGTFTPEQIKKEAE
                     RCLSCGATFVDTTLCVGCGMCATKCKFDAISLERTFDGHGEDYRDLKPILIKSVLKRK
                     LRIAIRKPGRLLKSAFVPKQD"
     gene            445..795
                     /locus_tag="PLQ29_07745"
     CDS             445..795
                     /locus_tag="PLQ29_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HPG86576.1"
                     /translation="MGSLHELSVVIEVVRTVEEFARENGVTRIDKLVLQIGELSSMIP
                     QYIEDCFPCAVDGTSMVDTKLEIEILPGNGRCRSCGAMFNLVANKGICPECGVKDAEV
                     ISGKEFMIKEIVAC"
     gene            complement(847..1755)
                     /locus_tag="PLQ29_07750"
     CDS             complement(847..1755)
                     /locus_tag="PLQ29_07750"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPG86577.1"
                     /translation="MYGIAAPFIGVLITMMNGVNSRLSSSVGALTAALTIHVAGIVII
                     HGILAARTAYGRAAGRPLGKASRLPFYLYLGGVVGVGTVFSCNYSYTVLDASLAVALG
                     LIGQTVFSILADATGFLGRERRPVSVRRLPGMALAVIGAIVIARNWRSDGVAMLIALG
                     SGLCTGLNALLNAEHGRRNGTLRSARWNYISGLTVTLIIVAATGPDWRSAAATVAEAG
                     PFLAAGGASLGVMVVLSMNLVFARLSAFRATLLLFSGQALSGVVMDAVVSGAFDPRKL
                     VGTIVVVAGLALDGAIGTGRKTAAKP"
     gene            1843..2568
                     /locus_tag="PLQ29_07755"
     CDS             1843..2568
                     /locus_tag="PLQ29_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPG86578.1"
                     /translation="MDASGRYIARYELSRIFSPELLAALRLRRYDPGAIIIRSGDPVD
                     EVLFFVDGRAKAYSTLDNGQRVLASFYLPFDAFGEAELFSSERYALTIEAITESACLC
                     LSAAALLGAAPSNHRLLAYLCGRLGAKLNDRFTAESINLRYPVERRLASYLLASSDDG
                     GIIAADDLGDLADYIGCSYRQLSRVVRDFRARGILSGRRGGLTVADRSALEPLAGDLY
                     TRSASVPPRVGRRLGVLERDDER"
     gene            complement(2623..>3322)
                     /locus_tag="PLQ29_07760"
     CDS             complement(2623..>3322)
                     /locus_tag="PLQ29_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG86579.1"
                     /translation="FQDPYASLNPRMTTRDIISEPMRIFTRAKILSLSRTEIDERVEQ
                     LMERVGLSRFFKNRYPHEFSGGQRQRIGIARALALKPDLVLCDEPVSALDVSIQAQIL
                     NLFKDLQEEFGLTYMFIAHDLAVIQYISNRIAVMYLGVIVELSDSAELYKKPLHPYTQ
                     ALLSAAPIPDPAVERKRQRIILTGDVPSPDKARTGCYFYDRCSKRMDKCAQSVPKLKD
                     AGSGHQVACFLYGD"
BASE COUNT          614 a         1090 c         1076 g          542 t
ORIGIN      
        1 tcaacaagga caacgtggat ctggacggat acgacaacat accccgccag cgcgccgccc
       61 acgccccggc caaggacgcc ttcaaggacg ttcgcggtac ctttaccccg gagcagatca
      121 agaaggaagc cgagcgttgc ctgagctgcg gcgccacctt cgtcgacacg accctgtgcg
      181 tcggatgcgg catgtgcgcc accaagtgca aattcgacgc cataagcctg gagcgtacgt
      241 tcgacggcca cggcgaggac taccgggacc tgaagcccat actcatcaag agcgtgctca
      301 aacgcaagct gaggattgcg atcaggaagc ctggccggct gctcaagtcg gcgttcgtgc
      361 ctaagcagga ctaggcctcc aatcggccag agactattcg gacgggggac gatggttccc
      421 ccgtccatcg tatctacggg agaggtgggg tccttgcacg agctgagcgt tgtcatagag
      481 gtcgtaagga ccgtcgagga gttcgccagg gagaacggcg ttacccggat agataagctg
      541 gtccttcaga taggcgagct atcgtcgatg atcccccagt atatagagga ctgcttcccg
      601 tgcgccgtcg acggcacctc catggtcgac accaagctgg agatagagat cctgcccggc
      661 aacgggcgct gcaggtcctg cggagctatg ttcaacctgg tggcgaacaa gggcatctgt
      721 cccgagtgcg gcgtcaagga tgcggaagtc atctcaggca aggagttcat gatcaaggaa
      781 atcgtcgcct gctaggcgac gagccgacga gccgacgagc gatccggcct tagcctcgcc
      841 tcccgcttac ggcttcgcgg ccgttttcct gcccgtcccg atcgcgccgt ccagcgccag
      901 cccggctacc acgacgatcg tgccgacgag cttccgaggg tcgaaggccc cggaaacgac
      961 ggcgtccatg accacgcccg acagcgcctg ccccgagaac aggagcaggg tcgccctgaa
     1021 cgccgacagc ctggcgaaca ccaggttcat cgacaggacg accatcacgc ccagcgaggc
     1081 gccgccggcg gccaggaagg ggccggcctc ggccaccgta gcggcggccg agcgccaatc
     1141 cggcccggta gccgcgacga tgatcagcgt cacggtcagg ccgctgatat agttccagcg
     1201 cgcgctcctg agcgtgccgt tcctccggcc gtgttccgcg ttgaggagcg cgttgaggcc
     1261 ggtgcatagg ccgctaccga gggctatcag catggcgacg ccgtcggagc gccagttcct
     1321 ggctatcacg atggccccga tcacggccag cgccatgccg ggcaacctcc ggaccgagac
     1381 gggacgcctc tcccggccaa ggaaacccgt cgcgtcggcg agtatggaga agaccgtctg
     1441 ccctatcagc ccgagcgcca ccgcgagcga ggcgtccaac acggtgtacg aatagttgca
     1501 cgagaagacc gtaccgacgc cgaccactcc gcccaagtac agatagaacg gcaggcggct
     1561 cgccttgccg agcggccgcc ccgcggcccg cccataggcg gtccgcgccg ccagaatgcc
     1621 atggatgatc acgatgccgg cgacgtggat ggtcagggcg gcggtcaggg ccccgactga
     1681 cgacgacagg cggctgttga cgccgttcat catggtgatg agcaccccaa tgaagggcgc
     1741 cgcgataccg tacatggttc ctcctgaata cacggctgat tatggcgtcg gggacttgca
     1801 tacgatagga catatgtcct atcgtcgctt aaaaggacga ccatggacgc atcaggtcgc
     1861 tatatagctc gctacgagct cagccgcatc ttcagccccg agctactcgc ggccctgcgc
     1921 cttcgacgct acgatcccgg ggccatcatc atccgctcgg gcgatcccgt cgacgaggtg
     1981 ctgttcttcg tcgacggacg ggccaaggcc tacagtacgc tggacaacgg ccagcgcgta
     2041 ctcgcctcgt tctacctgcc gttcgacgcg ttcggcgagg cggagctgtt ctcgtccgag
     2101 cgctacgcgt tgaccatcga ggccataacc gagtcagcct gcctctgcct gtcggcggcc
     2161 gcgctgttgg gcgcggcccc gagcaaccac cggctcctcg cctacctctg cggccggctc
     2221 ggggccaagc tcaacgaccg gttcaccgcc gagtccataa acctacgcta cccggtcgag
     2281 cgccggctag ccagctacct gctcgcctcg agcgacgacg gcgggatcat cgcggccgac
     2341 gacctcggcg acctggccga ctatataggc tgtagctacc gccaactgtc gcgggtcgtc
     2401 agggacttcc gcgcccgagg catactgtcg ggacggcgcg gcgggcttac agtagccgat
     2461 cgctccgcgc tcgagccgtt ggccggagac ctgtacaccc ggtccgcgtc ggtccccccc
     2521 cgggtaggtc ggcggctcgg cgtcctcgaa cgggacgacg aacgataaaa aacccgccgt
     2581 cgcgatcgac ggcgggccga agtacgattc tagcgcgacg tatcagtcgc cgtataggaa
     2641 gcaggccacc tggtggcccg agccggcgtc cttgagcttg ggtacgctct gggcgcactt
     2701 gtccatgcgc ttggagcagc ggtcgtagaa gtagcagcca gtcctggcct tgtcgggcga
     2761 cggtacgtcg ccggtcagga tgatgcgctg gcgcttccgc tcgaccgccg ggtccgggat
     2821 cggggcggcc gacagcaggg cctgggtgta cgggtgcagt ggcttcttat acagctccgc
     2881 cgagtccgac agctcgacga tgacgcccag gtacatcacc gcgatgcggt tggagatgta
     2941 ctggatcacc gccaggtcgt gggcgatgaa catgtatgtg aggccgaact cctcctgcaa
     3001 gtccttgaac aggttcagga tctgggcctg gatcgacacg tcgagggcgg agaccggctc
     3061 gtcgcagagc accaggtccg gcttgagggc cagggccctc gctatgccga tgcgctggcg
     3121 ctggccgccc gagaactcgt gcgggtagcg gttcttgaag aagcgcgaca gcccgacgcg
     3181 ctccatgagc tgctccacgc gctcgtcgat ctcggtcctc gacaaggaca ggatcttggc
     3241 cctggtgaag atcctcatcg gctcggagat gatgtcgcgg gtggtcatgc gcgggttgag
     3301 cgacgcgtac gggtcctgga ac
//
LOCUS       DAOPHV010000268         3312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_39955_length_3312_cov_5.325453, whole genome shotgun sequence.
ACCESSION   DAOPHV010000268 DAOPHV010000000
VERSION     DAOPHV010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39955_length_3312_cov_5.325453"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..75)
                     /locus_tag="PLQ29_07765"
     tRNA            complement(2..75)
                     /locus_tag="PLQ29_07765"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Ile,seq:cat)
     gene            206..1534
                     /locus_tag="PLQ29_07770"
     CDS             206..1534
                     /locus_tag="PLQ29_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPG86580.1"
                     /translation="MIKIPVISGDETMNVFIQRQCEALGKEYSPVFFKDKDEVVAYLK
                     YDLPEIKIIYFSDSTVEAREVLDEIGKDPWLHYGGVIAIHDNLEEKSLLDAMRDQNVI
                     AVLRRSEFERGFSRLMKILRQNKQILFQRGIQQHLLKNIAGSFVMDNDPLDITTYANL
                     VTNYLYNANLISRDTKEKLHVALLELLINAIEHGNCRIGYDEKTSWLEGNGDIMELIR
                     QKNRDPAVRARKVFFSYTISPESSRFMIKDEGEGFDWKARLAAQPDEPGLHGIGMKMA
                     SLYVSSLSYNDAGNEVSFEVRHQRNETNTIPAIFDSCQERAFNDGQYVCSEGEESDYL
                     YYIVSGTLYVYSKGKLVSALTPDDMFMGEMSFLLSNRRSATVVSKGKSVLIKISKQDF
                     VNLIKDNPHYGIFLARLLAQRLARLNLRTSRLNTEYLKLKEELASIRPAE"
     gene            complement(1591..2295)
                     /locus_tag="PLQ29_07775"
     CDS             complement(1591..2295)
                     /locus_tag="PLQ29_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HPG86581.1"
                     /translation="MDQLLTWLEGPVVDFNAALARLIFSLFACGLIGLEREMRRQTAG
                     WRTHIVIGLGATLLMLLSIWLPQSIGGDKGDPGRIAAQVVSGIGFLGAGAFVKIGNNV
                     KGLTTAATIWFVAGLGLAIGAGMWEIALIVLVLSLLVLTFLEPLEKKWFPSERLKLLQ
                     IWYEGSSLDRKSVYATLKTFRINVQSVDASLSVQKKQTRLSALVKVPVNVDLDTLFTE
                     LRKTGKVAKVRLHENY"
     gene            complement(2433..3299)
                     /locus_tag="PLQ29_07780"
     CDS             complement(2433..3299)
                     /locus_tag="PLQ29_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPG86582.1"
                     /translation="MKATRTTDSTSSIDSNVLSIYLKEINRIPLLSREDEDRYARAAA
                     AGDQAAKDMLVKGNLRFVVNVAKRYQNQGIPLSDLISEGNIGLINAIERYDVDKGYHF
                     ISYAVWWIRQAILKAICEKSRMIRLPLNRANELVQIEKARKYVPAGLSEDGEIREIAK
                     MLDMDASHVADLVNVSRDLVSLEAPVFDERDSSVLGDFVENDRYQSPDDHVIEQGLKD
                     DINDVLRTLTEKESEVLQYRFGLNGRRAMSLKEIGDRFNLTKERIRQIEKAALKRLSV
                     PGRTESLEGYVA"
BASE COUNT          651 a          987 c         1067 g          607 t
ORIGIN      
        1 ttgggcccac ctggacttga accagggacc gacggattat gagtccgcag ctctaaccaa
       61 ctgagctata ggcccgcgag gtaacgcgag cgtcaattat ccataattga agaaaaaagt
      121 caattgcgga gggtttcggg ccgatgctca agacggggac ggcggcttca ctgatcgccg
      181 tccggccatc ggaaggatgc tcggtatgat aaaaatcccg gtaatatccg gcgatgagac
      241 gatgaacgtg ttcatccagc ggcagtgcga ggccctgggc aaggaatact cgcccgtgtt
      301 cttcaaggac aaggacgagg tggtggccta cctcaagtac gacctgcccg agataaagat
      361 aatctatttc tcggattcta ccgtcgaggc gcgcgaggtg ctcgacgaga taggcaagga
      421 cccctggttg cactacggcg gcgtgatagc gatccacgac aacctcgagg agaagtcgct
      481 cctggacgcg atgcgcgacc agaacgtcat cgcggtcctc cggcgctccg aattcgagcg
      541 cggcttctcg cgactgatga agatactcag gcagaacaag cagatcctct tccagcgtgg
      601 catccagcag cacctgctca agaacatcgc ggggtcgttc gtgatggaca acgacccgct
      661 cgacatcacg acctacgcga acctggtgac gaactacctg tacaacgcca acctgatcag
      721 ccgcgacacg aaggagaagc tccacgtggc gctcctggag ctgctgatca acgcgatcga
      781 gcacggcaac tgccgcatag gctacgacga gaaaacctcc tggctcgagg gcaacggcga
      841 tatcatggag ctgatacgtc agaagaaccg tgacccggcg gtgcgcgccc gcaaggtgtt
      901 cttctcgtat acgatcagcc ccgagtcgag ccgcttcatg ataaaggacg agggcgaggg
      961 cttcgactgg aaggctaggc tggccgccca gcccgacgag cccggcctgc acggcattgg
     1021 catgaagatg gcgtcgctgt acgtaagcag cctgagctat aacgacgcgg gcaacgaggt
     1081 gtcgttcgag gtgcggcacc agcgcaacga gacgaacacg atccccgcca tcttcgactc
     1141 gtgccaggag cgggccttta acgacggcca gtacgtctgc tcagagggcg aggagtccga
     1201 ctacctatac tacatcgtgt cgggcacgct ctacgtgtac tccaagggca agctggtctc
     1261 ggcgttgacg ccggacgaca tgttcatggg cgagatgagc ttcctcctat cgaaccggcg
     1321 ctcggccacg gtcgtgtcga agggcaagag cgtcctgatc aagatctcca agcaggactt
     1381 cgtcaacctg atcaaggaca acccgcacta cggcatcttc ctggcgcgcc tcctggcaca
     1441 gcggctggct aggctgaacc tgcgtacctc gcggctcaac acggagtacc tcaagctgaa
     1501 ggaggaactc gcttcgatac ggcccgcgga gtagccgcgg gccgggcggg ccccgacccg
     1561 cccgggtctc ggaagggccc tatagtcgac ctagtagttc tcgtggagcc tgaccttggc
     1621 caccttgccg gtcttgcgca gctcggtgaa cagcgtgtcc aggtcgacgt tgaccggcac
     1681 cttgaccagg gccgacagcc tcgtctgctt cttctggacc gacagcgacg cgtcgacgga
     1741 ctggacgttg atgcggaacg tcttgagcgt ggcgtagacc gatttccggt cgagcgagct
     1801 cccctcgtac cagatctgca gcagcttgag ccgctccgac gggaaccact tcttctcgag
     1861 cggctcgagg aaggtgagta cgaggagcga caggacgagc acgatcaggg ctatctccca
     1921 catgccggcg cctatggcta agccgagccc cgctacgaac cagatcgtgg cagccgtggt
     1981 caggcccttg acgttgttgc ctatcttgac gaaggcgcca gcgccgagga agccgatgcc
     2041 cgatacgacc tgggccgcta tacgtcccgg gtcgcccttg tcgccgccga tggactgggg
     2101 cagccagatc gacagtagca tgagcagggt agcgccgagg ccgatgacga tgtgcgtgcg
     2161 ccagccggcc gtctggcggc gcatctcgcg ctcgaggccg atcaggccac aggcgaacag
     2221 gctaaatatg agtcgggcca gggccgcgtt gaagtcgacg accggaccct cgagccaggt
     2281 aagcagttga tccacgatgc agctccttgc cgcactattc tagcatgcgc ggcgaggagg
     2341 gtcgagccgt ggccgcgaat aaagcgcggc cgcccgggga ggcatctccg ggcggccgct
     2401 atatcgtgaa cgcgccctgg ggcgcggacg gatcaggcta cgtagccttc gagcgactcg
     2461 gtgcggccgg gcacggacag gcgcttgagg gcggccttct cgatctggcg gatgcgctcc
     2521 ttggtcaggt tgaagcggtc gccgatctcc ttaaggctca tggcgcggcg cccgttaagc
     2581 ccgaagcggt actggagcac ctcgctttcc ttctcggtaa gcgtccggag cacgtcgttg
     2641 atgtcgtcct tgagcccctg ctcgatcacg tgatcgtccg gagactggta gcgatcgttc
     2701 tcgacgaagt cgccgagcac gctcgagtcg cgctcgtcga agaccggggc ctcgagtgag
     2761 acgagatcgc gggagacgtt gacgaggtcg gcgacgtggc ttgcgtccat gtccagcatc
     2821 ttggctatct cgcggatctc gccgtcctcg gataggccgg cgggcacgta tttcctggcc
     2881 ttctcgatct gaacgagctc gttggcgcga ttgagcggga ggcggatcat gcggctcttc
     2941 tcgcagatgg ccttgagtat ggcctggcgt atccaccaga cggcgtagct gatgaagtgg
     3001 taacccttgt cgacgtcgta gcgctcgatg gcgttgatca gtccgatgtt gccctcgctg
     3061 atcaggtcgg acagagggat gccctggttc tggtagcgct tggccacgtt gacgacgaaa
     3121 cgcaggttgc ccttgacgag catgtccttc gcggcctggt cgccggcggc ggcggcgcgg
     3181 gcgtagcgat cctcgtcctc gcgagacagg agcgggatgc ggttaatttc cttgagatat
     3241 atggagagta cgttcgaatc gatggaagag gtggaatcgg tcgtgcgcgt ggctttcatg
     3301 cagttcctcc tg
//
LOCUS       DAOPHV010000269         3306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_40065_length_3306_cov_3.807444, whole genome shotgun sequence.
ACCESSION   DAOPHV010000269 DAOPHV010000000
VERSION     DAOPHV010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40065_length_3306_cov_3.807444"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..366
                     /locus_tag="PLQ29_07785"
     CDS             136..366
                     /locus_tag="PLQ29_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86583.1"
                     /translation="MVALIVGFVCIAFAIFACLPGTLDWWQDVLAFLRGSVPVLAAFI
                     GLIAVFIGVADIKDRIEAKKEEAEEASAEKKE"
     gene            463..891
                     /gene="rplM"
                     /locus_tag="PLQ29_07790"
     CDS             463..891
                     /gene="rplM"
                     /locus_tag="PLQ29_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPG86584.1"
                     /translation="MKTVFVKPAEVARSWFVIDAQGQRLGRVAAKAATLVRGKHKACY
                     TPHQEVGDYVVIVNADKIEVTGNKFNDKVYYRHTGYAGGLKKNTFASLIERHPTDPLE
                     IAIRGMLPKGPLGRKLAMNVKVYAGPNHPHAAQSPVAIEV"
     gene            904..1296
                     /gene="rpsI"
                     /locus_tag="PLQ29_07795"
     CDS             904..1296
                     /gene="rpsI"
                     /locus_tag="PLQ29_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPG86585.1"
                     /translation="MVRNLGIGTGRRKTAVARVYLREGTGKITVNNLELEKYFPIAEI
                     AGSVRQPLVVTASDNKFDIVINVVGGGPNGQAGACRHGIARALSQVDPASSIALRNNG
                     FLTRDPRMVERKKYGQRGARRRFQFSKR"
     gene            1320..1958
                     /locus_tag="PLQ29_07800"
     CDS             1320..1958
                     /locus_tag="PLQ29_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPG86586.1"
                     /translation="MARIVGIEKGASGAVKAVAVDGSFFIFRNVFLAAALMERGLSLP
                     FTERRDDDDPFVEVADEALDDAVAATEAEARALALLARAEQYRFGLERKLTARGFSRF
                     AIRVALDGLESVGLLSDERYASSWIRQRIRHKVEGPRSLAAALSVKGVDRRAVKAAMD
                     QVLEDGDTQRIIMRCAARLVDSGGEMADHRARLIELGWRPADVDEAIDSLES"
     gene            2028..2684
                     /locus_tag="PLQ29_07805"
     CDS             2028..2684
                     /locus_tag="PLQ29_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPG86587.1"
                     /translation="MDDDILTIEEVAKYLRVSERTVYDWAQKGEIPAGKIGTVWRFKK
                     SEIERWVNERLSSNKPVSNLHPVRMRNVLSPDRVVFLDYPRKRDALVALAERLAAAPQ
                     IKSKQELVSELLRREDLMSTAIGRNIAIPHVRLSSVTDLVVAVGVSKCDIQDFNAFDD
                     APVRLLFMIAAAYNQHAYYLQTLSYFSAKLKSNELREGILSAADPAAVYELLIAQDED
                     "
     gene            2844..>3306
                     /locus_tag="PLQ29_07810"
     CDS             2844..>3306
                     /locus_tag="PLQ29_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG86588.1"
                     /translation="MFLKSLEIFGFKSFADRSRVDFSEGISALLGPNGCGKSNVVDAI
                     KWVVGEQSARSLRADTMENIIFNGTESRKALNVAEVTLTISNEKGVLDLDVPEVAIKR
                     RLYRSGESEYFINNQPAKLKELKELFWDTGIGMSAYSVMEQGRIDQILSSKP"
BASE COUNT          690 a          973 c          997 g          646 t
ORIGIN      
        1 tatcaccatg ctttcgatgc tatccgtggt tttgatccat aagattcgga acactggagg
       61 cgatggccta tattgacgaa aatcgagttt cgggtatgct tatacccgct ttgtccctac
      121 tcatggagga tcataatggt agctttgata gtaggtttcg tatgtatcgc gtttgccatc
      181 ttcgcgtgcc ttccggggac cctggactgg tggcaggatg tgctcgcctt tctacgcggc
      241 tccgtgccgg tgctcgcggc gttcatcggc ttaatagccg tgttcatcgg cgtcgcggac
      301 ataaaggata ggatagaagc caaaaaagaa gaggccgagg aagcctccgc cgagaagaaa
      361 gagtagtcct ctcgtccgct tgacacgata tgcgatgtat gggattatac cgctcgccgt
      421 tttatacgct aacgaactcc acgaaggtga aggtatagat tgatgaagac cgtattcgta
      481 aagcccgccg aagtcgcccg ttcctggttc gtcattgacg cccagggtca gcgcctcggt
      541 cgcgtagccg ccaaggcggc cactcttgtg cgtggcaagc acaaggcctg ctacaccccg
      601 catcaggaag tcggcgacta tgtcgtgatc gtcaacgctg acaagatcga ggtaaccggc
      661 aacaagttca acgataaggt gtattatcgc cataccggct atgccggcgg gctcaagaaa
      721 aataccttcg cgtccctgat cgagcggcat cccacggatc ccctcgagat cgcgattcgc
      781 ggtatgctcc ccaaggggcc gctcggccgc aagctcgcga tgaacgtgaa ggtctatgcg
      841 gggccgaacc acccgcacgc ggcgcagtcg cccgtcgcta tcgaagtcta ggagaggtaa
      901 acgatggtta ggaatcttgg aataggtacc ggtagacgta agactgccgt ggcccgagtc
      961 tatctacgcg agggcacggg caagatcacc gttaacaacc tcgagctcga gaagtacttc
     1021 ccgatagccg agatagccgg ttccgtccgt cagcctctcg tcgtgactgc cagcgacaac
     1081 aagttcgata tcgtcattaa cgtggtcggc ggcggtccca acggccaggc cggcgcctgc
     1141 aggcacggca tcgcccgcgc cctcagccag gtcgaccccg catcgagcat cgcgctcagg
     1201 aataacggtt tcctgacccg cgatccgcgt atggtcgagc gcaagaagta cggacagcgt
     1261 ggagcgcgca ggcgcttcca gttctccaag cgctaagaca cgatggacga aacggcctga
     1321 tggccaggat cgtcggtatc gagaagggag cctccggcgc cgtcaaggcg gtcgccgtgg
     1381 atggctcctt ttttattttc cggaatgtct tcctggctgc ggcgctcatg gaacgcggcc
     1441 tttccttgcc gttcaccgag cgccgggatg acgacgaccc tttcgtcgag gtggcggacg
     1501 aagcgctcga tgacgccgtc gcggcgacgg aagccgaggc tagggcactg gctctgttgg
     1561 caagggcgga gcagtaccgt ttcggtctgg agcggaaact gaccgctcga ggcttctctc
     1621 ggttcgcgat ccgggtcgcc ctcgacggcc tcgagtcggt cgggctattg tctgacgagc
     1681 gctacgcctc gtcatggata cggcaaagga tacggcataa ggtcgaaggg ccgagaagcc
     1741 tagcggccgc tctgtcggtt aagggcgtgg atcgcagggc cgtcaaggcg gcgatggatc
     1801 aggtgctcga ggacggcgat acgcagcgga tcatcatgcg ctgtgcggca aggctggtcg
     1861 atagcggcgg tgaaatggct gaccaccgag cgcgcttgat cgagttgggc tggcggcccg
     1921 cggacgtgga cgaggctatc gactcgctcg agtcctagaa ataccttgac atttcatcac
     1981 ctttcttaca gaatgattgc aaatcatacc gtatggggag agccgcaatg gatgacgaca
     2041 tcctaactat agaagaagtg gccaagtacc tacgcgtttc cgagcgcacg gtctacgact
     2101 gggcgcaaaa gggagagatc cccgcaggca agatcgggac tgtctggcgc ttcaagaaga
     2161 gcgagatcga gcgctgggtt aatgagaggc tttcatcgaa caagccggta tcgaacctgc
     2221 acccggtgcg catgcgcaac gtcctatcgc ccgaccgggt cgtattcctc gactacccac
     2281 gcaagcgcga tgccttggta gccctggccg agcgcctcgc ggccgcgccg cagatcaaga
     2341 gcaagcagga gctggtctcc gagctgctca ggcgcgaaga cctgatgagc accgctatcg
     2401 gtcgtaacat cgccataccc cacgtgaggc tgtcgagcgt caccgacctc gtcgtagcgg
     2461 tcggcgtcag taaatgcgat atccaggact tcaacgcctt cgacgacgcg ccggtcaggc
     2521 tcttgttcat gatcgccgcc gcctacaatc agcacgcgta ctacctgcag actctgtcat
     2581 acttcagcgc caagctcaag tcgaatgagc tacgggaagg tatcctctcg gccgctgacc
     2641 cggccgccgt ctatgagctt ctgatagcgc aggacgagga ctaaaaccgg ggcccgatgg
     2701 cctagctacg gaggcgcccc gttcggggcg ccttcttcgt ctacggtatc cagggcgggc
     2761 ttccgttgcg ccaggagcgg cttccgtgct actattcgtc gctttcatac atagaaatcc
     2821 aagctagaac gaggtgccgt cacgtgttcc tgaaaagcct cgagatattc ggctttaaga
     2881 gcttcgccga tcgatcgcgc gtagacttct ccgagggcat ctccgccctg ctcggcccca
     2941 acggttgcgg taagtcgaac gtcgtcgacg cgatcaagtg ggtcgtcggc gagcaatccg
     3001 cgcgctcact gcgcgccgac acgatggaga acatcatctt caacggcacc gagtcccgca
     3061 aggcgctcaa cgtggccgag gtcaccctga ccatctcgaa cgagaagggc gtcctggacc
     3121 tcgacgtgcc cgaagtcgct atcaagcgcc gtctctaccg atccggcgag agcgagtact
     3181 tcatcaacaa tcagccagcc aagctcaagg agctcaagga gctgttctgg gacaccggca
     3241 tcggcatgag cgcctattcg gtgatggagc agggccgcat cgaccagatc ctttcgtcca
     3301 agcccg
//
LOCUS       DAOPHV010000270         3303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_40103_length_3303_cov_4.119458, whole genome shotgun sequence.
ACCESSION   DAOPHV010000270 DAOPHV010000000
VERSION     DAOPHV010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40103_length_3303_cov_4.119458"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PLQ29_07815"
     CDS             complement(<1..407)
                     /locus_tag="PLQ29_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPG86589.1"
                     /translation="MITVCVASGKGGAGKTSLSAAIAERLGPSCVFADCDVDAANGAI
                     ALGAKGTRSEPYFAGPGFEIDPAACTSCGACRAACRFGAIERANGSFRVVPELCERCG
                     ACLSRCPAEAIRTYEKQAGELLVSDSRLGMRIV"
     gene            complement(404..1297)
                     /locus_tag="PLQ29_07820"
     CDS             complement(404..1297)
                     /locus_tag="PLQ29_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPG86590.1"
                     /translation="MSDAGTTVIAVASGKGGTGKTTVAAHLAIRGAREMPTVLVDLDV
                     EAPDASGYFKGVRRSGDASEVSVLVPRLVEARCTGCGLCAKACRFGAILSIGGVVTID
                     PKVCKGCGSCVNACPAAALEEIPMTVGQTSSFDADGLAMLEGRMAIGDIRSTAVIEAA
                     KKRAAESRAALQVRDCPPGVSCPATHAIEGADYVVLVAEPTEFSIHDLGAAARLARGR
                     GIRAGVVINKDGFGDADVAGFCEREGIPVIGRIAFERERAASGAAAELWSDDAAISGE
                     MDAILARALAAAGARTVGGRR"
     gene            complement(1294..1671)
                     /locus_tag="PLQ29_07825"
     CDS             complement(1294..1671)
                     /locus_tag="PLQ29_07825"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HPG86591.1"
                     /translation="MDRTGPAGAGPMTGRGLGQCGAGAGRGFGRGRGLGNGRGMGRGL
                     GARGLGWFSVGYDGVGDGATGAANAYGAANAGAAYAADLWKALETRAATLRAELARAD
                     ALLAGETGMAHGDAKEPGKDSGR"
     gene            2110..>2378
                     /locus_tag="PLQ29_07830"
     CDS             2110..>2378
                     /locus_tag="PLQ29_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPG86592.1"
                     /translation="MSQKVQASFFDAKGVAFVGYSKRHAAYSAEIRKEFEARGSTVYP
                     VNPASGPYDVEVMGSVADIPGSPELAVVITNKSRNDEILSALAAKG"
     assembly_gap    2379..2388
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2558..2914
                     /locus_tag="PLQ29_07835"
     CDS             2558..2914
                     /locus_tag="PLQ29_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86593.1"
                     /translation="MKPHPYGPAYRPLLALSGLFALGAVLTLVPNPNASWPNILGYSS
                     LCTFAPGATFGCAMLAAITCTVRARLARRWTAPAALPIVAIAALAIGLGVSTVAWAGV
                     KADYTEAVSGASATTE"
     gene            complement(2989..>3303)
                     /locus_tag="PLQ29_07840"
     CDS             complement(2989..>3303)
                     /locus_tag="PLQ29_07840"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPG86594.1"
                     /translation="YLYFDGAPADAIAEGDHLDSFPSGHAIMAFAGAAFTATLYAERY
                     PDAPERLPATIAAYALAALTVGLRVASGSHFASDVLAGAAIGSAFGFAVPFAASRLGW
                     LD"
BASE COUNT          481 a         1174 c         1103 g          535 t
ORIGIN      
        1 tgcacgatgc gcatgccgag ccgcgagtcc gataccagga gctcgccggc ttgtttctcg
       61 taggtccgaa tcgcctcggc cgggcatcgg ctcaggcagg cgccgcaacg ctcgcagagc
      121 tcaggcacga cgcggaacga cccgttcgcg cgctcgatgg cgccgaagcg gcacgcggcc
      181 cggcaggcgc cgcacgaggt acaggcggcc gggtcgatct cgaagccggg gccggcgaag
      241 tacggctcgc tgcgcgtgcc cttcgcgccg agcgctatgg ctccgtttgc cgcgtcaacg
      301 tcgcagtcgg cgaaaacgca ggaagggccg agacgttcgg ctatcgcggc cgatagcgag
      361 gtcttgcctg ctccgccctt gccgctggct acgcacacgg tgatcatcgc cgccctccga
      421 ccgtccgcgc gccggcggcg gccagcgccc gcgccagtat cgcgtccatc tcgcctgaga
      481 tcgcggcgtc atcgctccac agctcggcgg cggcgccgga tgcggcccgc tcccgctcga
      541 aggcgatgcg gccgatgacc gggatgcctt cccgctcgca gaatccagcg acgtcggcgt
      601 cgccgaagcc gtccttgttg atgacgacgc cggcccgaat gccgcgaccg cgcgccaggc
      661 gggcggccgc gcccaggtcg tgtatagaga actcggtagg ctcggcgacc agtacgacgt
      721 agtcggcgcc ctcgatcgcg tgggttgccg ggcacgagac gccgggcggg caatcgcgta
      781 cctgcagggc ggcgcgcgac tcggcggcgc gcttcttggc ggcctctatg acggcggtgg
      841 agcggatgtc gcctatagcc atccggccct cgagcatcgc tagcccgtcg gcgtcgaacg
      901 acgaggtctg tccgaccgtc atcgggatct cttcgagcgc ggcggccggg caggcattga
      961 cgcaggagcc gcagcctttg cataccttgg ggtctatcgt aacgacgccg cctatggaca
     1021 ggatggcgcc gaagcggcag gccttggcgc agaggccgca tccggtgcag cgcgcctcga
     1081 cgagccgcgg tacgagcacc gagacctccg aggcgtcgcc cgagcggcgg acgcccttga
     1141 agtagcctga agcgtccgga gcctcgacgt cgaggtcgac gagcacggtc ggcatctcgc
     1201 gggctcctcg tatcgctagg tgggcggcga ccgtcgtctt gccggtgccg cccttgccgc
     1261 tcgcgacggc tatcaccgtc gttccggcgt cgctcatctg ccggaatcct ttcccggctc
     1321 cttggcgtcg ccatgggcca tgccggtttc gcccgccagt agcgcgtcgg ccctggccag
     1381 ctctgcgcgc agcgtcgccg cccgcgtctc gagcgctttc cacaggtcgg ccgcgtacgc
     1441 ggctccggcg ttcgcggcgc cgtaggcgtt ggcggcgccg gtagcgccgt ccccgactcc
     1501 gtcatacccg acgctgaacc agccgaggcc cctagccccg aggccgcggc ccataccgcg
     1561 tccgtttccg agcccccgtc ctctcccgaa accgcgcccc gctccggcgc cgcactgtcc
     1621 gagcccgcgg ccggtcatcg gtcccgctcc cgccggtccg gttctatcca ttcctggcat
     1681 gatagcctcc tactgcgtat cgaagatcgt tgaacgccga cggcgcttga ctgtaattgg
     1741 catatgctct attagacttt atgcgaataa taggcatatg cctgttattt gtcaaccggt
     1801 tacagagatt ttcctaacta tgccttgaca gctcggaccg gagcgtcctc aatgactcca
     1861 tgagaatcat cgcgttggta gacgacctgt gcccgaaggg cggcttccgc ggcaatcggg
     1921 aaggcgttcc cggcgtcgag tggctttcat gcggcatgcg ggccgagctg tgagccgggc
     1981 ggctagtata tagtcgcctt gaccgcggaa tgatctatag atacattgtc ctaacattcg
     2041 ggccataggc cgaatcacgt cactcgtcat gtcccgttcg gcggaagcgc tcgggagaga
     2101 ggatttatca tgtcccagaa ggttcaggct tcgttcttcg acgcgaaggg cgtcgcgttc
     2161 gtcggctact ccaagcggca cgcggcctat tccgcggaga tacgcaagga attcgaagcg
     2221 cgcggctcta ccgtctaccc ggtcaacccg gcttccgggc cgtacgacgt cgaggtcatg
     2281 ggctccgtag ccgatatccc cggatcgccc gagctggcgg tcgtcatcac gaataagagc
     2341 aggaacgacg agatcctgtc cgcgctggcc gccaagggnn nnnnnnnngc tcgaaggtct
     2401 cggccgactc ggcgacgctc gagcgctgcg cctcgctcgg catggaaggc gtcgtcgtgt
     2461 gcccgctcca ggcgataggc ggcggcttcc accgcttcca cggctggctg tccggcgtgc
     2521 ccaaggtgcc cgtcgcccgg gcctgagggg cgtagccatg aagcctcacc cgtacggccc
     2581 ggcctacagg ccgctcctgg cgctgagcgg cctcttcgcc ctcggggccg ttctcaccct
     2641 cgtgcccaac ccgaacgcct cgtggccgaa catactcggc tactcgtcgc tgtgcacctt
     2701 cgcgccgggg gccaccttcg gctgcgcgat gctcgcggcg atcacctgca ccgtccgcgc
     2761 ccgcctagcg cgccgctgga cggcgccggc ggccctgccc atcgtcgcga tagccgccct
     2821 ggccataggc ctgggcgtct cgaccgtcgc gtgggcaggc gtcaaggccg actataccga
     2881 ggcggtctcg ggcgcctcgg cgacaaccga ataggccgcc cgtcgcgacc gagtcggccg
     2941 cccgtcgcga ccgagtcggc cgcccggggc ggcgacgccg ttccactgtc agtccagcca
     3001 gccgagcctg gacgcggcga acggcaccgc gaagccgaac gccgagccga tcgcggcgcc
     3061 ggccagcacg tccgacgcga agtgcgagcc ggacgccacg cgtaggccga cggtcagcgc
     3121 cgccaacgcg taggcggcta tcgtcgccgg caggcgctcc ggcgcgtcgg ggtatcgctc
     3181 cgcgtatagc gtggccgtga aggccgcgcc ggcgaacgcc atgatcgcgt gtcccgacgg
     3241 gaacgagtcc aggtggtcgc cctcggctat cgcgtcggcc ggcgcgccgt cgaagtacag
     3301 gta
//
LOCUS       DAOPHV010000271         3300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_40146_length_3300_cov_4.383051, whole genome shotgun sequence.
ACCESSION   DAOPHV010000271 DAOPHV010000000
VERSION     DAOPHV010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40146_length_3300_cov_4.383051"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLQ29_07845"
     CDS             <1..208
                     /locus_tag="PLQ29_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86595.1"
                     /translation="ASSAAASRAAWLVAAVGLVAAAGLVAAVGALSGRSLLLEQDWAE
                     TASASTIAAPDIPYSRDRLACIVV"
     gene            256..1497
                     /locus_tag="PLQ29_07850"
     CDS             256..1497
                     /locus_tag="PLQ29_07850"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPG86596.1"
                     /translation="MDDIWPRLGIAFDEPVRDPLWRDVMMPTEFEPILTSPDFVKLSR
                     ILQLGPTHLVYPGATHTRRAHSIGVFELAKRSLATVLSRSGPVAGLDGAAARGFLVAA
                     LCHDLGHFPYAHSLKELPLRDHESLTADIVRGSLRPAVEAAGADPDLVAAIVDQGLPA
                     PAGSSAVALRLFRSMLSGVLDPDKLDYLNRDAWACGVPYGQQDVDFVLQRLGLDESGR
                     PGVTERGVMAVEALLFSKYQMYRAVYWHKTVRAATAMIKKAVIVALDDGAVEPDELYG
                     LDDGGFYSLMAGPGRDRSGLIASVLHGAVLRPCLELAFDPGLVAHARAADLSLRPRLE
                     EAIAGDAGAETVIVDVPEPISFESDLPIIGTGSGFSDYATVFRPEVVASFTRSLRVIR
                     VFSDGSPERVSAAALEALGGR"
     gene            1494..2279
                     /locus_tag="PLQ29_07855"
     CDS             1494..2279
                     /locus_tag="PLQ29_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HPG86597.1"
                     /translation="MSAAWEPSPELVDTAAGRWMGYVKRFMAAAGSGTHRYGLIRDGD
                     SVLVGLSGGKDSLALLLFLELRRRRVKDRYTLKAARVDWGAPPAAPEDEAALAAFCAW
                     LGVPFFEERRPMPESLAERGLSCYACSRERRRVLFELAEREGCGVVALGHHLDDFAET
                     ALMNVCKHGRLDPMRPQAVFFGRFRLVRPLCEARESALRAVATRLGLPVVRNRCPLAD
                     SSQRRAYKAALAELAKIDTLVRENVCKAASMAASPPEESHGDP"
     gene            2266..2967
                     /locus_tag="PLQ29_07860"
     CDS             2266..2967
                     /locus_tag="PLQ29_07860"
                     /inference="COORDINATES: protein motif:HMM:NF022179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HPG86598.1"
                     /translation="MEIHNLMEDLVVKTVDELFDAEAKGSTSAWCTCRQCRMDVACYV
                     LNRLKPEYVLSGRGVAYSENDYGEKLQRVADVVSLVKEGWSRINAAPRPHHHCGPGGD
                     EGSSGQVKGPAFNLRPIMGRLFNGDNFEPIVDAEVSLVDEAGLVMMIDDNWSNPYRLA
                     RNTNGTFTFWPRPVPCERAGERRRFSMTITASPEGFEELSHHMEFELSSDEAPVYQLS
                     TQSARRLQDLYVFAK"
     gene            complement(2977..3222)
                     /locus_tag="PLQ29_07865"
     CDS             complement(2977..3222)
                     /locus_tag="PLQ29_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86599.1"
                     /translation="MTIETRWLVYPDGERQETDRLLRVDELVDMNGYPLPLPPPSDRM
                     IAYRVFKIRRVEERGELDVLQYLDLVPAAELRELRVR"
BASE COUNT          501 a         1118 c         1118 g          563 t
ORIGIN      
        1 ggcctcgagc gcagccgctt cgcgcgcggc ctggctcgtc gccgcagtcg ggctcgtcgc
       61 cgcggccggc ctcgtcgccg cggtcggcgc gctatccggg cgctccctgc tcttggagca
      121 ggactgggcg gagacggcga gcgctagtac gatagcggcg ccagatatac cgtatagtcg
      181 agatcggctc gcttgcatcg tggtatagta tctccagacc gagccggtcg atcaaccggt
      241 tccgacggag gcatcatgga cgacatctgg ccgcgcctcg gcatcgcgtt cgacgaaccc
      301 gtcagggatc ccctctggcg cgacgtgatg atgccgaccg aattcgaacc tattctaacg
      361 agcccggatt tcgtcaagct gtcgcgcatc ctccagctcg gcccgacgca cctggtctat
      421 cccggcgcca cccataccag gcgggcccac tccataggcg ttttcgagct ggccaagcga
      481 tcgctcgcaa ccgtgctgtc gcgctcaggc cccgtagccg ggctcgacgg ggccgcggct
      541 aggggcttcc tcgtcgcggc gctctgccac gacctcgggc actttccgta cgcccattcc
      601 ctcaaggagc tgccgctgcg cgaccacgag agccttacgg ccgacatcgt gcgcggtagc
      661 ctgcgaccgg ccgtggaagc cgccggcgcc gatcccgact tggtggcggc catcgtcgac
      721 cagggcctgc cggctccggc cggctcgagc gctgtcgcgc tccggctgtt ccgctcgatg
      781 ctatccggcg tgctcgaccc ggacaagctc gattacctga atcgcgacgc ctgggcctgc
      841 ggcgtgccct acggccagca ggacgtcgac ttcgtgctgc agcggctggg gctcgacgag
      901 tcgggccggc ccggggtgac cgagcgcggc gtgatggccg tcgaggcgct gctgttctcc
      961 aagtaccaga tgtaccgggc ggtctactgg cataagacgg tgcgcgccgc gacggcgatg
     1021 atcaagaagg cggtcatcgt cgcgctcgac gacggcgcgg tcgagccgga cgagctgtac
     1081 gggctcgacg atggcggctt ttactcgctg atggccgggc cggggcggga ccggagcggt
     1141 ctcatcgcgt cggtgctcca cggggcggta ctcaggccct gcctggaact ggccttcgac
     1201 cctggcctgg tcgcgcacgc ccgggccgca gacctgagcc tccggccgcg cctcgaggaa
     1261 gccatagccg gggacgccgg cgccgaaacg gtcatcgtcg acgtacccga gcccatatct
     1321 ttcgagtccg acctgcctat aatcgggacc ggctccggct tctccgacta cgcgacggta
     1381 ttcaggcccg aggtggtcgc gtccttcacg cgttcgctgc gggtgatacg cgtcttctcc
     1441 gacggctctc ccgagcgcgt ctcggccgcc gccctggaag cgctgggcgg ccggtgagcg
     1501 ctgcctggga gccgtctccg gaactcgtcg acacggcggc ggggcggtgg atgggttacg
     1561 ttaagcgctt catggcggcc gccggatccg gtacccatcg ctacgggctg atacgcgacg
     1621 gagactcggt cctcgtcggc ctgtccggcg gcaaggactc gctggccctg ctcctattcc
     1681 tcgagctgcg caggcggcgg gtcaaggacc gttatacgct caaggcggcc cgcgtcgact
     1741 ggggtgcgcc gcccgccgcg ccggaggatg aggcggcgct cgcggcgttc tgcgcctggc
     1801 tcggcgtgcc gttcttcgag gagcgccggc cgatgcccga gtcgctggcc gaacggggct
     1861 tgtcgtgcta cgcctgctcg agggagcggc ggcgcgtcct gttcgagctg gccgagcgcg
     1921 agggctgcgg cgtcgtggcg ctcgggcatc acctggacga cttcgccgag acggcgctga
     1981 tgaacgtgtg caagcacggc cggctcgacc cgatgcggcc ccaggccgtc ttcttcgggc
     2041 gcttcaggct cgttcgcccg ctctgcgaag cgcgcgagtc ggccctgagg gcggtggcga
     2101 ccaggctcgg cctgccggtc gtacggaacc gttgcccctt ggccgactcg agccaacgcc
     2161 gggcctacaa ggcggcgctt gcggaactcg ctaaaatcga tacactggta cgggagaacg
     2221 tatgcaaggc ggcgtcgatg gcggcgtcgc ccccggagga atcgcatgga gatccataac
     2281 ctgatggaag acctcgtcgt caaaacggtt gacgagctgt tcgacgccga ggccaagggc
     2341 tcgacgagcg cgtggtgtac ctgcaggcaa tgccgcatgg acgtagcctg ctacgttctc
     2401 aatcgcctca agccggagta cgtgctgtcc gggcggggcg tcgcgtactc ggagaacgac
     2461 tacggagaga agctacagcg agtcgcggac gtcgtgtccc tggtcaagga gggctggtcc
     2521 aggatcaacg cagccccgcg cccccatcac cactgcggac ctggcggcga cgaggggtcc
     2581 tccggccagg tcaaggggcc ggccttcaac ctcaggccga tcatggggcg cctgttcaat
     2641 ggcgacaatt tcgagcccat cgtcgacgcg gaggtgtcgc tcgtcgacga ggcgggtctg
     2701 gtcatgatga tagacgataa ttggagcaat ccgtaccgcc tggccaggaa caccaacggt
     2761 accttcacct tctggccgcg gcctgtaccc tgcgagcggg cgggggagcg acgacgcttc
     2821 tcgatgacca taacggcgag ccccgagggt ttcgaggagc tgtcgcatca tatggaattc
     2881 gagctcagct ccgacgaggc gcccgtctac caactatcca ctcagtcggc tcgcaggctc
     2941 caggacctgt acgtgttcgc caaatagcgg cattgactac cgtaccctca gctccctgag
     3001 ctcggccgct ggcaccaggt ccaggtactg gagcacgtcg agctcgcctc tctcctcgac
     3061 ccgcctgatc ttgaacacgc ggtaggcaat catcctgtcg ctcggcggcg gcaggggcag
     3121 gggatagccg ttcatatcga ccagctcgtc gactcgtagc aacctatcgg tctcctggcg
     3181 ttctccgtcg ggatagacga gccagcgggt ttcaatcgtc ataggcggat tctagccgga
     3241 cgatagcgcc cgtgcaagcc tcgagcctcg agcctccagc ctcccggcgt cccggcgtcc
//
LOCUS       DAOPHV010000272         3295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_40228_length_3295_cov_4.464815, whole genome shotgun sequence.
ACCESSION   DAOPHV010000272 DAOPHV010000000
VERSION     DAOPHV010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40228_length_3295_cov_4.464815"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLQ29_07870"
     CDS             complement(<1..611)
                     /locus_tag="PLQ29_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPG86600.1"
                     /translation="MERIKVMVVDDSAVVRQTLKELLDSDPGIEVLAVASDPIFALKK
                     LETERPDVIVSDVEMPRMDGLSFLRKIMETSQIPVVICSSKTEAGSDNAIKAMEYGAV
                     EIIQKPKLGTKQFLEESKVEICDVVKAAYAARHRRRAPAAPIRDVAPKLTADVMLPAP
                     GSGHIIETTEKVVVVGASTGGTEALREFLEVLPEDTPGIVIVQ"
     gene            complement(631..1473)
                     /locus_tag="PLQ29_07875"
     CDS             complement(631..1473)
                     /locus_tag="PLQ29_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HPG86601.1"
                     /translation="MLAESQTAVGISDRQFKRLSAFIEKELGIKMPDTKRVMLESRLQ
                     KRLRALRIDTYDEYIDRVFSGDDAELIHMIDVVTTNKTDFFREPDHFEILTKRLLPKA
                     LERDGERGTMSFWSAGCATGEEPYTLAMVLEEFAVAHRGFDYRIIASDISTSALDAAI
                     HAVYDANRVIPVPPSYKKKYLLRSRDPAKNEVRIKRELRDKVGFLRINFMDDSFPFDG
                     QFDVIFCRNVIIYFDRATQERILGRMCRYLRTGGNLILGHSETLTSMDLPLRGLAPTV
                     YERL"
     gene            complement(1582..>3295)
                     /locus_tag="PLQ29_07880"
     CDS             complement(1582..>3295)
                     /locus_tag="PLQ29_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86602.1"
                     /translation="ALSYRPTDPDACYLSALARLSRGDGVGPAAGLLEVALSVGGFRL
                     YDEGGARLLYAGILERTGRPAEALRFLSGLPPSAESLFIGAKAALAMGDEAEARAAVL
                     SSLRRYPFDPRPLLAWLRYDERPSLKAEDAAVVASGFAALDRLKSVDPSVLAALAPYA
                     SSADRARLMLREYRAAGGRDADSTALALRYGLIDERRAISELLSGDYRPTVSALESVY
                     SLLASDESRSAFGREFAGYTGAVYRDDDRDGYPESVTEYRDGLPSSWSLDADQDGEPE
                     FAASFDSGAPARLSARSGSTTVVVAYAPWPYAENVSYADTSGSRMYSFGPSSMPLPLL
                     SLSRIAGLAGAPYAVSRSGYGLPTEAAAKRAAYAMIESGPRGASTTVELSDGQASRSW
                     WVDEWRRVGRTVYRNGVPDDERMDVDADGRFESRRVWARGPDGVPAPAYAEADLDGDG
                     LYEYRETLSGPSVQSWDYDGDGAIDLSLERLPGGRSLYRVFARDGGLTEAMLEGGRLS
                     SVVERGAPVPLVDDSGGLVRWIGAKPFDFGGRVPPTGRGSRDGVMYAVHDIGGRLYAQ
                     VVRE"
BASE COUNT          563 a         1090 c         1076 g          566 t
ORIGIN      
        1 tgctggacga tgacgatgcc cggcgtgtcc tccggtagca cctcgaggaa ttcccgaagc
       61 gcctcggtac ccccggtaga cgcgcctacc accacgacct tctcggtcgt ctcgatgata
      121 tgccccgagc cgggggcagg cagcattacg tcggcggtga gtttcggagc cacgtcgcgt
      181 ataggagcgg ctggcgcgcg tctacgatgc cgcgccgcat aggcggcctt gacgacgtcg
      241 cagatctcga ccttcgattc ctccaggaac tgcttcgtgc cgagcttcgg cttctggatg
      301 atttcaacag cgccgtattc catcgccttg atggcgttat ccgaaccggc ctcggtcttg
      361 ctcgagcaga tgaccaccgg tatttggctg gtctccataa tcttccgcag gaacgaaagg
      421 ccgtccatcc tcggcatctc gacgtcggat acaatgacgt cggggcgctc ggtttcaagc
      481 ttcttgagcg cgaagatggg atcggaggct acggccagga cctctatgcc cgggtcagaa
      541 tccaggagtt ccttgagcgt ctgcctgact acggcggagt cgtctacgac catgaccttg
      601 atgcgttcca tctggctccc ctcgggtcaa ctataagcgc tcgtagacgg tgggcgcaag
      661 cccgcgcagc gggaggtcca tgctcgtcag cgtctcggag tgtccgagga tgaggttgcc
      721 gccggtccgc aggtaccggc acatgcgccc gagtatgcgc tcctgcgtcg ctctgtcgaa
      781 gtagatgatc acgttcctac agaatatcac gtcgaactgc ccgtcgaacg ggaaactgtc
      841 gtccatgaag ttgatgcgca ggaagccgac cttgtcgcgt agctctcgct tgatgcgcac
      901 ctcgttcttc gcgggatcgc ggctcctgag caggtatttc ttcttgtacg acggcggcac
      961 agggattacc ctattggcgt cgtagacggc gtgtatcgcc gcgtcgaggg cgctcgtcga
     1021 aatgtccgag gcgatgatgc ggtagtcgaa gcccctatgg gctacggcga actcctcgag
     1081 gaccatggcg agggtatagg gctcttcgcc ggtggcgcag cccgctgacc agaatgacat
     1141 cgtgccgcgt tcgccgtcgc gctcgagcgc cttgggtagg agccgcttag tcaggatctc
     1201 gaaatggtcc ggctccctga agaaatcggt cttattggtg gtgacgacgt ctatcatgtg
     1261 gatcagctcg gcgtcgtccc cggagaagac gcggtcgatg tactcgtcgt aggtatcgat
     1321 ccgcaacgcg cgcagccgct tctgaaggcg gctctcgagc attacccgct tggtgtcggg
     1381 catcttgatg ccgagttcct tctctatgaa ggccgacaag cgcttgaact gcctgtcgga
     1441 gataccgacg gcggtttgac tctcagcgag catgggcaac ggcatcctcc atacgcgtac
     1501 cgtgggcgtt cgtcgatatc agtgtattgg ctacgcgcag ggtccgcaag caggaatcgc
     1561 cgggaccccc gcgtgctccg cctattcccg tacgacctgg gcgtacaacc ggcccccgat
     1621 atcgtgcacg gcgtacatga cgccatctcg cgaaccgcgc ccggttggcg ggacccggcc
     1681 gccaaaatcg aagggcttcg cgcctatcca gcgcaccagc ccgccggagt cgtcgacgag
     1741 tggtaccggc gccccgcgct cgaccacgct cgacagccgc cctccctcga gcatcgcctc
     1801 ggtcaagccg ccgtcgcgcg cgaatacgcg atacaggcta cggccgcccg gcaggcgttc
     1861 caggctcagg tcgatggcgc cgtcgccatc gtagtcccaa gactgtacgg acgggccgga
     1921 cagcgtctca cgatactcgt agagcccgtc gccgtccagg tcggcctcgg cgtaggccgg
     1981 cgccggcacg ccgtcagggc cgcgggccca gacccggcgc gactcgaaac gcccgtcggc
     2041 gtcgacgtcc atacgctcat cgtcaggcac gccgttccga tagacggtcc tgccgacgcg
     2101 gcgccactcg tccacccacc acgagcgcga ggcctggccg tcgctcagtt ccaccgtggt
     2161 agaagcgccg cgaggtccgg attcgatcat agcgtacgcg gcccgtttgg cggcggcctc
     2221 ggtcggtagc ccgtagccgc tccgcgatac ggcgtacggc gcccccgcga gcccggcgat
     2281 gcggctcagc gacagcagcg ggagcggcat cgatgagggg ccgaaggaat acatcctcga
     2341 accagaggtg tccgcgtacg atacgttctc agcatagggc cacggcgcat acgcgacgac
     2401 gacggtcgtc gagcccgatc gcgccgacag cctggctgga gcgccggagt cgaaagacgc
     2461 ggcaaattca ggctcgccat cctggtcggc gtcgagggac cacgacgaag gtaggccgtc
     2521 acggtactcg gtgaccgatt caggatagcc atcgcggtcg tcgtcgcggt acacggctcc
     2581 cgtatacccg gcgaactcgc ggccgaacgc ggatcgggac tcgtcgctgg ccagcaggga
     2641 atagacggac tcgagcgccg ataccgtcgg gcgatagtcg ccggacagga gctcggatat
     2701 agcccgccgc tcgtctatca gcccgtagcg cagggcgagc gccgtagagt cggcatcacg
     2761 gcccccggcg gcgcggtact cccggagcat cagcctggcc cggtccgcgg atgacgcgta
     2821 cggcgccagc gcggcgagga ctgacgggtc gaccgacttc agccggtcga gggccgcgaa
     2881 gcctgaggcg accacggcgg cgtcctcggc tttcagggac ggccgttcat cgtatcgtag
     2941 ccaggcgaga agggggcgcg ggtcgaaggg ataccttcgt aacgacgata gcacggccgc
     3001 gcgggcctcg gcctcgtcgc ccatggccag ggccgccttg gctccaatga acagcgactc
     3061 ggcgctcggg ggcagtcccg acaggaagcg tagcgcctcg gccggccgtc cggttcgctc
     3121 gagtatcccc gcgtacagca gcctcgcgcc gccctcgtcg tacagcctga agccgccgac
     3181 ggatagcgcg acctcgagca acccggccgc cggaccgact ccgtcacctc gcgacagtcg
     3241 agcgagggcc gacaggtaac aggcgtccgg gtcggtcggc cggtagctca gggcc
//
LOCUS       DAOPHV010000273         3291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_40292_length_3291_cov_3.782138, whole genome shotgun sequence.
ACCESSION   DAOPHV010000273 DAOPHV010000000
VERSION     DAOPHV010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40292_length_3291_cov_3.782138"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLQ29_07885"
     CDS             complement(<1..625)
                     /locus_tag="PLQ29_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HPG86603.1"
                     /translation="MADLSVSFLGLKLRNPVLVASSGLTSTKEGVRRAAEAGAGAIVL
                     KSLFEEQLRAEIADIASDAESHPEAEAFLAGMGLSEGEGEYVALVKAAKATTDVPIIA
                     SLNCSGDSLWPELAARVAAAGADAIELNLGVVPMDPATPAAHIEDRLVALVGSVASQV
                     RVPVCSKLGSSYTNVGNLASRLAKAGSRGLTLFNRFYRMDVDLETMSL"
     gene            complement(711..1109)
                     /locus_tag="PLQ29_07890"
     CDS             complement(711..1109)
                     /locus_tag="PLQ29_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPG86604.1"
                     /translation="MDELRIGLTGRKELVVEERHTASHLGSGGAPVYATPMMVLAMEE
                     AALAAVDHLLGDGQATVGVGLDVRHLAATPLGMRVTATAELVGADGRMLTFRVEARDE
                     VDLIGEGTHVRAVVDLERFRDKLAKKARRA"
     gene            complement(1110..2696)
                     /locus_tag="PLQ29_07895"
     CDS             complement(1110..2696)
                     /locus_tag="PLQ29_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HPG86605.1"
                     /translation="MIDRHRHGWWREAVFYQIYPRSFMDSNGDGVGDLRGIESRLDYL
                     AWLGVDALWISPFFKSPMRDFGYDISDYEDVDPIFGSLDDFRSLLDAAHRLGIRIVVD
                     LVVNHSSDEHPWFKAARSSRDDPKHDWYIWKPVEGRRPPNNWISLFEQRSAWYPNEAT
                     GEWYLATFTRNQPEFNWRNPELREAIYRVARFWLDLGADGFRMDVATAYFKDAEFRSN
                     PPSPVLVPDLLQRHLYDRNRPEVHGVFREFRALADSYGDRMLIGETHGQDPALAASCH
                     GERNDELHMAFNFDFLFRPWGAESFRRSAERWYEVLPDGAWPNFTLSNHDQKRHYYRY
                     RSGADSDARARVAAAMLLCLRGTPFLYYGEEFGMSCERVPRKALRDPLGLSTWPLPLG
                     RDAERTPMQWDASPNAGFSDAEPWLPVNRDYKDKNVEAQRADPGSLLTWYRRLIRLRS
                     LEPALRIGELRWLDAPAGVMAFRRSFGNREIDVWLNFVARAKTVTTLGGSVLDDPGET
                     IAAGDRELGPYEVLITAREA"
     gene            complement(2719..>3291)
                     /locus_tag="PLQ29_07900"
     CDS             complement(2719..>3291)
                     /locus_tag="PLQ29_07900"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPG86606.1"
                     /translation="DYEKEHLLNIAILASIAINNAFLFEMTTTDMMTKLRMKHYFYTV
                     LLEKMEESAFSEKPLSVIMADIDSFKRFNDTYGHSCGDEVLKGVAKLIQSQTRSVDVA
                     ARYGGEEFCMLLPDTDAATAKLIAERIRRSVEGMGFDYEGQKIAVTLSLGIAQYDQNR
                     DLTGKSVIDRADKALYASKQAGKNRVSVSP"
BASE COUNT          536 a         1065 c         1108 g          582 t
ORIGIN      
        1 cgaggctcat cgtctccagg tcaacgtcca tacggtaaaa gcggttgaat agcgtcaggc
       61 cgcgcgagcc ggccttggcc aggcgcgagg cgaggttgcc gacgttggta tacgaggagc
      121 ctagcttgga gcataccggc acgcgcacct gcgaggctac ggatccgacg agggctacca
      181 gcctatcctc gatgtgcgcc gccggagtcg ccggatccat cggtacgacg cccaggttga
      241 gctcgatcgc gtcggctccg gccgcggcga cgcgggcggc cagctccggc cagagcgagt
      301 cgccggaaca gttcaggctg gctatgatcg gaacgtcggt cgtcgccttg gcggccttga
      361 cgagcgccac gtactcgccc tcgccctcgc tcaggcccat gccggccagg aaggcctcgg
      421 cctccggatg cgactcggcg tcgctggcga tatcggctat ctcggcgcgg agctgctcct
      481 cgaatagtga cttgagcacg atggcgccgg cgccggcctc ggccgccctg cggacgcctt
      541 ccttggtcga agtcagcccg gacgaggcga cgagtacggg gttacgcaac ttgagcccga
      601 ggaatgatac gctcagatcg gccatggtcg ctccttagtg attgaagatg acgcgttagc
      661 gctatgctca gtatacggag cccgcggccc ggcggcaaat ccggaaagga tcaagcccgg
      721 cgcgccttct tggccagctt gtccctgaaa cgctccaggt cgacgacggc ccgtacgtgg
      781 gtgccctcgc cgatcaggtc gacttcgtcg cgcgcctcga cgcggaacgt cagcatgcgg
      841 ccgtcagccc cgacgagctc ggcggtcgcc gtgacgcgca tgccgagcgg ggttgccgcc
      901 aggtgtcgca cgtcgaggcc gactccgacg gtcgcctggc cgtcgcctaa caggtgatcg
      961 accgctgcca gggcggcctc ctccatggcg aggaccatca tcggcgtggc gtagaccggc
     1021 gcgccgcccg agccgaggtg gctggcggta tgccgctcct cgaccacgag ctcctttcgc
     1081 ccggtcagcc ctatccgtag ttcatccatc taggcctccc tcgccgtgat caggacctcg
     1141 tagggaccga gctcgcggtc gcctgcggct atcgtctcgc ccgggtcgtc caggacgcta
     1201 ccgcccaagg tcgtcacggt cttggcccga gctacgaagt tcagccagac gtcgatctcg
     1261 cgattcccga acgaacggcg gaaggccatc acgccggccg gggcgtcgag ccagcgcagc
     1321 tcgccaatcc gcagggcggg ctccagggag cggagccgta tcaggcgcct gtaccaagta
     1381 agcagggagc cgggatcggc gcgctgggcc tccacgttct tgtccttata gtccctattg
     1441 accgggagcc agggctccgc gtcgctgaag ccggcgttcg gcgaggcgtc ccactgcatc
     1501 ggggtgcgtt cggcgtcgcg gccgaggggc aggggccagg tggacaggcc gagcgggtcg
     1561 cgcagggcct tcctcgggac gcgttcgcag ctcatgccga actcctcgcc gtaatagagg
     1621 aagggcgtgc cgcgcaggca gaggagcatc gccgcggcga cgcgggcccg agcgtcggaa
     1681 tcggcgcccg agcggtagcg atagtaatgg cgtttctggt cgtggttcga cagcgtgaag
     1741 ttcggccacg ccccgtcggg caagacctcg taccatcgct cggccgagcg gcggaacgac
     1801 tcggcgcccc aggggcggaa caggaagtcg aagttgaagg ccatgtgcag ctcgtcgttc
     1861 cgctcgccgt ggcacgaggc ggcgagggcg ggatcctgcc cgtgggtctc gcctatgagc
     1921 atgcggtcgc cgtatgagtc ggccagcgcc cggaactccc tgaagacgcc gtgcacctcg
     1981 ggccggttgc ggtcgtagag gtggcgctgc aggaggtcgg gcacgagtac gggactcggc
     2041 gggttggagc ggaactcggc gtccttgaag tacgccgtcg ccacgtccat cctgaagccg
     2101 tccgctccga ggtccagcca gaagcgggcg acccggtaga tcgcctcccg tagctcgggg
     2161 ttgcgccaat tgaattcggg ttggttgcgc gtgaaggtgg ccaggtacca ctcgccggta
     2221 gcctcgttcg ggtaccaggc ggaacgctgc tcgaacaggc tgatccagtt gttcggcggc
     2281 ctacggccct cgacgggctt ccagatatac cagtcatgct tcggatcgtc ccttgaggat
     2341 cgagccgcct tgaaccacgg atgctcgtcg gacgaatggt tgacgaccag gtcgacgact
     2401 atcctgatac cgaggcggtg ggccgcgtcg agcagggacc tgaagtcgtc cagggagccg
     2461 aagatagggt cgacgtcctc gtagtccgag atgtcatagc cgaagtcgcg catcgggctc
     2521 ttgaagaagg ggctgatcca cagcgcgtcg acgcccagcc aggccaggta gtcgagcctg
     2581 gactcgatac cgcgcaggtc tccgacgcca tcgccgttcg agtccatgaa gctacgcggg
     2641 tagatctggt agaagaccgc ctcgcgccac cagccgtggc ggtgtctgtc tatcatcgct
     2701 ctcacctccg gccgggcgtc agggcgaaac ggacacccgg ttcttgcccg cctgcttcga
     2761 cgcgtacagc gccttgtcgg ccctgtctat caccgacttg ccggtcaggt cgcggttttg
     2821 gtcgtactgg gctatgccga gcgacagggt gacggctatt ttctgccctt cgtagtcgaa
     2881 gcccatgccc tcgacgctgc ggcgtatcct ctcggcgatc agcttcgcgg tggccgcgtc
     2941 ggtatcgggc aagagcatgc agaattcctc gccgccgtag cgcgcggcca cgtcgaccga
     3001 ccgggtctgg ctctggatca gcttggcgac gcccttgagc acctcgtcgc cgcagctatg
     3061 cccataggtg tcgttgaagc gcttgaacga gtcgatatcg gccatgatca ccgacagcgg
     3121 cttctccgag aaggcgctct cctccatctt ctcgagcagc accgtataga aatagtgctt
     3181 catcctgagc ttcgtcatca tgtcggtcgt cgtcatctcg aacaggaagg cgttgttgat
     3241 ggctatcgac gccagtatgg cgatattgag gaggtgctcc ttctcgtaat c
//
LOCUS       DAOPHV010000274         3288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_40335_length_3288_cov_3.109187, whole genome shotgun sequence.
ACCESSION   DAOPHV010000274 DAOPHV010000000
VERSION     DAOPHV010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40335_length_3288_cov_3.109187"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1154)
                     /locus_tag="PLQ29_07905"
     CDS             complement(63..1154)
                     /locus_tag="PLQ29_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86607.1"
                     /translation="MSEGKGRRKIFTVLVAVVLIAVYFFVFSEPLRPELSAAPRWTID
                     LSASVASAEASAQDRVDASAVSFSTASRFGYFGTDGSIGFVGEATGPVPMSDSSYVSA
                     ELVPGGTNYSLRSPVGARVAELDASAPFYSSGRLFSAQADGSGVAAYDGKGNRLWSYT
                     FPCQLSAFSAGEGLAVGGTVDGWLEGVDSGGRRAFSFAPGGSRLQVVLGLGVSRSGNW
                     IAAVTGVDRQRLVVLGRGGADYRVASHRYLDSDYREPVRVVVLDDDRHVLYRRADGIG
                     VWSVDGAVDELLPVKADDFDVSLDAERGLALLVARRGKKAEVVAFRLPATLLGRVPLP
                     DGSEYVRLIGSSVYFGSGDSISRFDFMEE"
     gene            1207..1683
                     /gene="cdd"
                     /locus_tag="PLQ29_07910"
     CDS             1207..1683
                     /gene="cdd"
                     /locus_tag="PLQ29_07910"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPG86608.1"
                     /translation="MVYSIVPFRQYEALRGNGQKEASMERTALYELAAEAATKSYSPY
                     SRFRVGAALLCADGTVVCAANVENRSYGLAICAERSALVSAIAQGKTEFTELVISTPD
                     SDYPVSPCGACRQFISEFVGADFPIRFGCSKDSYVDSTMGELFPHDALHELKDRTE"
     gene            complement(1711..2526)
                     /locus_tag="PLQ29_07915"
     CDS             complement(1711..2526)
                     /locus_tag="PLQ29_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86609.1"
                     /translation="MPKITQEQRNRYAAKVKEYKAMADGILAKEQSLLNVLKQPNAQG
                     QGYVRVSLAEQMLVLTSYQILINSLSVSLLGIKNEDSLSEARKSVGRAIKYLEDIVTG
                     YVDASFSEYEQNLEAIAEIPYEKRYALLRKIGFAIGEIEEGYGANSKWKWSFVELWGK
                     LAVVAKNSLDLKKAFLDMDLASPNRMITLSYLAFIKKQLQSTADRYREKYEVSSMKMD
                     DFKQAILFLNALKRIHVVFGERTEAEELKRKIEIWSAKLESDQKKREEEAKKR"
     gene            complement(2526..>3288)
                     /locus_tag="PLQ29_07920"
     CDS             complement(2526..>3288)
                     /locus_tag="PLQ29_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPG86610.1"
                     /translation="RKYGDDLAAVLARRTESAERLASLENWEEDRAGLERQVAEIEAD
                     VNRRAELLTSARKEASVRLERAVVGVIATLGMPNARFVVRIERFPPAGGKVVVGPTGA
                     DNLEFYFSANKGEPLRPLADVASGGELSRFMLALKTVLAEDGGAPTMVFDEIDAGIGG
                     EVALGVGSHLQRLAASRQVLCITHLASIAARADNHLMVEKRVEGDRTVTRLARLDGPG
                     RVREVSRMLSGDAESRASLDHAAELVAKLGNARGT"
BASE COUNT          569 a         1090 c         1036 g          593 t
ORIGIN      
        1 cgatggcggc ggccaggacg acggcgcccg ccgcgacgac tcgaacggcc cgtagcgaca
       61 cgctattcct ccatgaagtc gaatctggat atagagtccc cggagccgaa atacaccgag
      121 gagccgatga gcctgacgta ctcgctgccg tcgggcaagg gtacgcggcc gagcaacgtc
      181 gccggaaggc ggaacgcgac gacctcggcc ttcttaccgc gcctggcgac gagcagggcc
      241 aggccgcgct ccgcgtccag cgacacgtcg aagtcgtcgg ccttgacggg tagcagctcg
      301 tcgacggcgc catcgaccga ccatacgccg atgccgtcgg cccggcggta gagcacgtgc
      361 ctatcgtcgt cgagcacgac gacgcggacg ggctccctgt aatccgagtc gaggtagcga
      421 tggctcgcca cacggtagtc ggcgcctcca cgcccgagca ccacgagccg ttgccggtcg
      481 acgccggtga ctgccgctat ccaattaccg gacctggaga cgccgagccc gagcaccacc
      541 tggagacggg agccccctgg tgcgaacgag aaagccctgc gcccgcccga gtcgacgcct
      601 tcgagccagc cgtcgacggt tccgccgacg gcgaggcctt cgccagcaga gaaggcgctc
      661 agctggcacg gaaacgtgta ggaccatagg cggtttccct tgccgtcgta ggcagcgacg
      721 ccgctgccgt cggcctgcgc ggagaacagc cgccccgacg agtaaaacgg ggcgctcgcg
      781 tcgagttcgg cgacgcgggc ccccacaggg gagcgtagcg agtagttcgt gccgccggga
      841 acgagctcgg cgctgacgta cgaggagtcc gacatcggga ctggtcccgt cgcctcgccg
      901 acgaagccga tggatccgtc cgtaccgaaa tagccgaaac ggctcgcggt cgagaaggag
      961 acggccgacg cgtcgacgcg gtcctgggcc gaggcttcgg ccgacgcgac cgacgccgag
     1021 aggtctatcg tccatcgggg cgcggcgctc agctctggcc gcaagggttc ggagaataca
     1081 aagaagtaga cggcgatcag cacgaccgcc acgaggacgg tgaatatctt ccgtcgcccc
     1141 ttcccctcgc tcatgcttcc ctccgcggca atcgttcgat gctcgtcatt ggcattcgcc
     1201 ccgtcgatgg tatatagtat cgtcccattc cgtcaatacg aagcgctacg cgggaatggg
     1261 cagaaagagg catctatgga acgtaccgcg ctatacgagc tggcggccga ggctgctacc
     1321 aagtcctact cgccttattc gaggttcagg gtcggggcgg ccctgctgtg cgccgacggt
     1381 accgtcgtct gcgccgccaa cgtggagaac cgttcctacg gcctggccat ctgcgccgag
     1441 cgttccgccc tcgtatcggc catcgcccaa ggcaagaccg aattcaccga gctcgtcatc
     1501 tccactccgg actcggacta tccggtcagc ccatgcggtg catgcaggca gttcatcagc
     1561 gaattcgtcg gggccgactt cccgatccgg ttcggttgct ccaaggactc ctatgtcgat
     1621 tcgacgatgg gggagctctt cccccacgac gcgctacacg agctcaagga cagaaccgag
     1681 tgatgcccgc ggagcgcgga gcgggggccg tcagcgcttc ttggcttctt cttcgcgctt
     1741 cttctggtcg ctctcgagct tggcggacca tatctcgatc ttgcgcttca gctcctcggc
     1801 ctccgtccgc tcgccgaaca cgacgtggat acgcttgagg gcgttcagga agaggatggc
     1861 ctgcttaaaa tcgtccattt tcatggacga gacctcatac ttctcgcgat agcggtcggc
     1921 agtcgactgc aattgcttct tgatgaaggc caggtaggac agcgtgatca tccggttggg
     1981 cgacgcgagg tccatgtcga ggaaagcctt cttcagatcg aggctgttct tggccacgac
     2041 cgcgagcttg ccccatagct cgacgaagga ccatttccac ttcgagttgg cgccgtaccc
     2101 ctcctcgatc tcgcctatcg cgaagcctat cttccgtagc agcgcgtagc gcttctcgta
     2161 ggggatctcc gcgatggctt cgaggttctg ctcgtactcg gagaacgacg cgtcaacgta
     2221 gccggtgact atatcttcca ggtacttgat ggcacgaccg acgcttttcc tggcttccga
     2281 cagcgagtcc tcgttcttga tgccgagcag cgagaccgag aggctgttga tcaggatctg
     2341 gtacgaggtc agaacgagca tctgctcggc gagcgatacg cgcacgtagc cctggccctg
     2401 ggcattcggc tgcttcagga cgttgagcaa ggattgttcc ttggcgagga tgccgtcggc
     2461 catcgccttg tattccttga ccttggcggc gtacctgttc ctctgttcct gcgttatctt
     2521 tggcattacg ttcctcgagc gttaccgagc ttggccacca gttcggccgc gtggtcgagc
     2581 gacgcgcgag actcggcgtc gcccgatagc atgcgcgaga cctccctcac gcggcccggg
     2641 ccgtcgagcc gcgcgagccg ggtcactgtg cggtcaccct ctacgcgttt ctccaccata
     2701 agatgattat cggctcgcgc ggctatcgac gcgagatggg taatgcaaag aacctgcctc
     2761 gaggcggcga gccgttgcag gtgcgaaccg acgccgagcg cgacctcgcc accgatgccg
     2821 gcgtcgatct cgtcgaaaac catggtcgga gcgcctccgt cctcggcgag gacggtcttc
     2881 agggccagca tgaagcggga tagctcgccc ccggacgcca catcggccag cggccgcagc
     2941 ggctcgccct tgttggcgga gaagtagaat tccaggttgt cggcgccggt cggcccgacg
     3001 acgaccttgc cgcccgcggg agggaaacgc tcgatcctca cgacgaagcg ggcgttcggc
     3061 atgcccaggg tcgcgatgac gcctaccaca gcccgttcga gccgtacgga ggcctctttc
     3121 ctagcgctcg tcaggagctc agcgcgtcgg ttcacgtccg cctctatctc ggcgacctgg
     3181 cgctcgaggc ccgccctatc ctcttcccag ttctcgaggc tggcgagccg ctccgccgat
     3241 tcggtcctgc gggcgagcac ggccgccagg tcgtcgccat acttgcgc
//
LOCUS       DAOPHV010000275         3278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_40492_length_3278_cov_4.999379, whole genome shotgun sequence.
ACCESSION   DAOPHV010000275 DAOPHV010000000
VERSION     DAOPHV010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40492_length_3278_cov_4.999379"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1645)
                     /locus_tag="PLQ29_07925"
     CDS             complement(<1..1645)
                     /locus_tag="PLQ29_07925"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPG86611.1"
                     /translation="MIESDVLVIGAGLSGLTCAALLAERGLSVRVLEACATPGGSCSS
                     FRRGAVTYDLGAALLFGFDGGFSPHRYLMDELGEPIDVYRHEAPYSVRYGERRIVFHR
                     DVDRFIDELAEAFPGTRDSLRAFYDELYALHPLIAGSSASYVPPSEMRLKDLAPAKGA
                     DPRDSLKALTLLAKSAESLARKHLRDVSLIRFFDKLTSTYCYTTMPETPAVLAVTMFV
                     ENHEGGAYSIAGGPAMLAGRLEKAVEDRGGSIEYGAPASRILFDGRRAVGAATEDGRE
                     FRARDVVFTGALSQLASGLVPERLLPRRWKKRVLGYEMTYRNFMVYGTVDRAALPPDA
                     MPIEVFIDNKEAIDEGDVTLYLESLEDPRLAPEGRCSFMLLGPTFSAWPAPGSAAHRD
                     PAYEAAKQAEGRRILGLVDRRWPGFAAAVGDAVLASPTTVERYLRKPGGVVAGPKQAL
                     GQALLKRPGARSPWPGLWFAGEWTTMGTGTPAVTVSGIGAADRVLRKRGLPEFRNAPR
                     QRSAVRVLPAGTPGNVPASAAGVEASRRQRSRGRCPASAA"
     gene            complement(1712..2518)
                     /locus_tag="PLQ29_07930"
     CDS             complement(1712..2518)
                     /locus_tag="PLQ29_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG86612.1"
                     /translation="MDATKPAIAGSERRTLHYLNSKFKASIFEVVSRSELLSATVAGL
                     NRRFLDMTGLFKRVGVSLKASMSAFSATFDEAGARVEEADRSFSSIESVFDEAYSLSE
                     ALRKEAANAGEQLAVIDDIAETMSILSLNASIQAARAGGAGKAFAVVASEIRRHTVST
                     KESIERTGRSVDFLVRTIASLAADMDRIRDEVQSGSRVMKELVEIIERERVELSSVNA
                     DLEAVDRGFEDYEAIKAALERMIGQSAVSKTEIERILLSYQRHLDAIERT"
     gene            complement(2523..>3278)
                     /locus_tag="PLQ29_07935"
     CDS             complement(2523..>3278)
                     /locus_tag="PLQ29_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HPG86613.1"
                     /translation="NPAEYIGVILFDGLCLGEEIMRELMVAEDFPIPLVGGAAADELA
                     FEATYVASGARMSSDGLVLLVLRMKVPFYYDHFVHCRPAGSSMVVTKSEPSRRVVWEI
                     DGKPAAVRYAELLGLPGPEAVGVMDFSRRPFGVAIGDTVYVRSPNAVVDGTGLQFYCY
                     IEAGTTVSLLEPGDILENAREALGEANRRLGEVRGALLFNCVLRYLELKASRSTDAFN
                     DIFAAAPFIGFNTFGEELFTHHNQTLTAIFIGR"
BASE COUNT          511 a         1132 c         1123 g          512 t
ORIGIN      
        1 cggcagcgct cgccgggcaa cgtccccgcg agcgctgccg gcgcgacgcc tcgacgccgg
       61 cagcgctcgc ggggacgttg cccggcgtcc cggccggtag cacgcgcacc gccgaacgct
      121 ggcgcggcgc gttgcggaat tcgggcagac cgcgcttcct gagcacgcgg tcggcggcgc
      181 cgatgcccga gacggtcacc gccggcgtgc cggtgcccat cgtcgtccat tctccagcga
      241 accacagccc gggccacggc gagcgggcgc ccggccgctt gagcagggcc tgcccgagcg
      301 cctgcttcgg ccccgctacg acgccgccgg gcttgcgcag gtagcgctcg acggtggtcg
      361 ggctggccag caccgcgtcg ccgacggcgg ccgcgaagcc gggccagcgg cggtcgacga
      421 ggcccaggat gcgccggccc tcggcctgct tagccgcctc gtaggcggga tcgcgatggg
      481 cggccgagcc gggggcgggc caggcggaga atgtcggccc gagcagcatg aaggagcatc
      541 gcccctccgg agccagtctc gggtcctcga gagattccag gtagagcgtg acgtcgccct
      601 cgtcgatcgc ttccttgttg tcgatgaaca cctcgatggg catcgcgtcg gggggcaggg
      661 ccgccctgtc gacggtgccg tagaccatga agttgcggta ggtcatctcg tagcccagga
      721 cgcgcttctt ccagcgcctc ggcagtagcc gctcgggcac gagccctgaa gccagctgcg
      781 acagcgcgcc ggtgaagacc acgtcgcgcg cgcggaactc gcggccgtcc tcggtcgcgg
      841 ccccgaccgc gcgccggccg tcgaacagga tacggctcgc gggggcgccg tactcgatcg
      901 agccgccgcg atcctccacg gccttttcga gccggccggc gagcatcgcc gggcccccgg
      961 ctatcgagta cgccccgccc tcgtggttct cgacgaacat cgtgaccgcg agcaccgcgg
     1021 gcgtctccgg catcgtggtg tagcagtagg tcgaggtcag cttgtcgaag aaccgtatca
     1081 gcgacacgtc gcgcaggtgc ttgcgggcga gcgactcggc gctcttggcc aatagggtca
     1141 gcgccttgag cgagtcgcgc gggtccgctc ccttggcggg cgccaggtcc ttgagccgca
     1201 tctcgctcgg cggcacgtac gaggccgacg agccggctat gagcgggtgg agggcgtata
     1261 gctcgtcgta gaacgcgcgc agcgagtcgc gggtgccggg gaaggcctcc gccagctcgt
     1321 cgatgaagcg gtcgacgtcg cggtggaaca cgatgcggcg ctcgccgtag cgcacgctgt
     1381 acggcgcctc gtggcggtaa acgtctatgg gctcgccgag ctcgtccatc aggtagcggt
     1441 ggggagagaa gccgccgtcg aagccgaaaa gcagggccgc gccgaggtcg taggtgaccg
     1501 ctccgcgcct gaaggacgaa caggagccgc cgggcgtcgc gcaggcctcg agcacccgta
     1561 ccgatagtcc gcgctcggcg agcagcgcgg cgcaggtcag gccggacagg ccggcgccga
     1621 tgactagtac atcgctttct atcatgggaa tacgactata tacgcggcgg cgcgaggccg
     1681 caagaaaaaa acgcggcgcg gccccgcggc gctatgtccg ctcgatcgcg tcgagatggc
     1741 gctggtacga gagcaggatg cgctctatct cggtcttcga taccgccgac tgcccgatca
     1801 tgcgctccag ggccgccttg atggcctcgt agtcctcgaa gccccggtcg acggcctcga
     1861 ggtccgcgtt gaccgacgat agctccacgc gctcgcgctc gatgatctcg acgagctcct
     1921 tcatgacgcg ggagccggac tgcacctcgt ccctgatcct gtccatgtcc gcggccagcg
     1981 acgctatcgt cctcacgagg aaatcgacgc tcctgcccgt ccgctcgatg gactccttgg
     2041 tcgagaccgt gtgccgcctg atctccgacg ccacgacggc gaaggccttg ccggcgccgc
     2101 cggccctcgc ggcctggatg gacgcgttga gcgataggat gctcatggtc tcggcgatgt
     2161 cgtcgatgac cgccagctgc tcccccgcgt tcgccgcttc cttcctgagc gcctcgctca
     2221 gcgagtacgc ctcgtcgaag acggactcga tcgaggagaa gctccggtcg gcttcctcga
     2281 cgcgcgcgcc cgcctcgtcg aaggtcgccg agaacgcgct catgctggcc ttgagcgaga
     2341 cgccgactcg cttgaatagt cccgtcatat cgaggaatcg cctgttgagc cccgcgaccg
     2401 tcgcggatag cagctcgctg cgcgatacga cctcgaatat cgatgccttg aacttgctgt
     2461 tcaggtaatg gagcgtcctc cgctccgatc cggcgatagc gggcttcgtc gcgtccatct
     2521 tcctacctcc cgatgaagat cgccgtcagc gtctggttgt ggtgcgtgaa tagctcctcg
     2581 ccgaaggtat tgaacccgat gaacggcgcg gccgcgaata tatcgttgaa ggcgtcggtc
     2641 gacctcgagg ccttgagttc caggtaccgc agcacgcagt tgaacaggag cgctccgcgt
     2701 acctcgccga gccgccggtt cgcttccccg agggcttccc tggcgttctc gagtatatcg
     2761 cccggctcga gcaggctgac ggtcgtgccc gcctcgatat agcaatagaa ctgcaggccc
     2821 gttccgtcga cgaccgcgtt cgggctcctg acgtagaccg tgtccccgat ggcgacgccg
     2881 aacgggcgcc gcgagaaatc catgaccccg acggcctccg ggcccggcag gccgagaagc
     2941 tcggcgtagc gcacggcggc gggcttgccg tcgatctccc agaccacgcg gcgagacggc
     3001 tccgacttcg tcacgaccat ggaggagccg gcggggcggc aatggacgaa atggtcgtag
     3061 tagaacggga ccttcatcct gagcacgagc agcacgaggc cgtcggacga catcctggcg
     3121 cccgaggcga cgtaggtggc ctcgaaggcc agctcgtcgg cggccgctcc cccgacgagc
     3181 ggtatcggga agtcctccgc gaccatgagc tcgcgcatga tctcctcgcc gaggcacagc
     3241 ccgtcgaaca ggatcacgcc gatatactcg gccgggtt
//
LOCUS       DAOPHV010000276         3277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_40508_length_3277_cov_4.610490, whole genome shotgun sequence.
ACCESSION   DAOPHV010000276 DAOPHV010000000
VERSION     DAOPHV010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40508_length_3277_cov_4.610490"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="PLQ29_07940"
     CDS             <1..970
                     /locus_tag="PLQ29_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPG86614.1"
                     /translation="LGAIVIVAGMLSALFMNDTMCLVLAPVVAETSLRAGRNPTPYLA
                     ATAIASNVGSAATIIGNPQNMLIGARSGVPFSEFAFRLAPPALIGLVVCWLVVQLAFP
                     GEFRRRGRAVVDGRAGRATSAGPLVAVRLDKTLAVKCAIASAAMLLGFIAGVPVALAA
                     MLPAALMLASTRLESERLLSGVDFNLLVFFGGLFVITDAAARTVAFAWLERTALTGAA
                     TSPWLFASATAAVSNVVSNVPAVMMLSPIASCAADPRSAWLLLAMASTFAGNLTLIGS
                     VANLIVAEGAASRGARLGFVDYLKAGLPVTLITLAIGTAWLAMTAS"
     regulatory      1011..1121
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1110..2135
                     /locus_tag="PLQ29_07945"
     CDS             1110..2135
                     /locus_tag="PLQ29_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HPG86615.1"
                     /translation="MLTHTHGARVLLAAAAISFAAIATSCVQKDDAQLVVYAYDSFAS
                     EWGPGPIVIPAFEKATGITVELVVPGDAGQVLARAVDEKRSPRADVIVGIDGNLLPKA
                     LAAGVLAAYEPKGSASVPARLRLDDDWRLTPYDWSSFCVMWDSEKLAVPPASLEDLTK
                     PEYGRKLILMDPRTSTPGLGFVEWTAAIYGDGLSGYWKRLAPSILTLSPGWDTGYGLF
                     TAGEAPLVISYTTSAAYHAEYETAGRYKALEFADGHPIQVEGAGIVVGARHREAAEAF
                     IDFMLGDEFQAALPLTNWMYPVNEGVVLPPSYEASKKPAKTVSAGLAPNQLDEAVRTA
                     LDALQGR"
     gene            2145..>3277
                     /locus_tag="PLQ29_07950"
     CDS             2145..>3277
                     /locus_tag="PLQ29_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPG86616.1"
                     /translation="MKAVRSRRASLPAAVLFPALGAALLVPLGAAFMPLFDGGAPANL
                     ARAFADPRFSRSFAYGLSQAAASAALAAAIGLPGAFLIARRTFPGKRLLTALSAVPFC
                     VPPLIIAIGFVLYYGREGFLNRTLMASFRLDQPPIGFLYSFAGVVMAHGFYNFPVVMR
                     FVGDAWATAPRRHEDAARLLGASERRVFMTVTLPSILPSIGASMSLVFLMCFYSFVIV
                     LLFGGAGVGTPEVELYRAARFEFDRPLASAFALVETVVAMGALALYAAFERRAAGDRE
                     ESERVAPNGFRSSGGIALAVLYGAFIAVCFVGPLASIVAESLAIRNASRVGSSLGLGN
                     YVSLFTRRGFVPALANTALLGLLCAALASVAGFTLSIGLKRARS"
BASE COUNT          448 a         1154 c         1069 g          606 t
ORIGIN      
        1 cctcggcgcc atcgtcatag tcgcggggat gttgtctgcg ctgttcatga acgacacgat
       61 gtgcctcgtg ctcgcccccg tcgtcgccga gacgagcctg cgcgccggcc gtaacccgac
      121 gccctacctg gccgccaccg cgatagcctc gaacgtgggc tcggccgcga ccatcatcgg
      181 gaatccgcag aacatgctca taggcgcgag gagcggcgta ccgttctctg aattcgcgtt
      241 caggctcgcc cctccggccc tcatcggcct ggtggtatgc tggctggtcg tacagctggc
      301 cttcccgggc gagttcaggc gacgaggccg ggccgtcgtg gacgggcggg ccggtcgcgc
      361 tacgagcgcc ggcccgctcg tagcggtgcg gctcgacaaa acgctggccg tcaagtgcgc
      421 catcgccagc gcggccatgc tcctgggttt catcgccggc gtaccggtgg cgttggccgc
      481 catgctgcca gccgcgctga tgctggcgtc gaccaggctg gagtcagaga ggctcctctc
      541 tggcgtcgac ttcaacctgc tcgtcttttt cggaggcctc ttcgtgatca ccgacgcggc
      601 ggcgaggacc gtggccttcg cctggctcga gcggacggcc ctgaccgggg cggcaacctc
      661 gccgtggctc ttcgcctcgg ccacggccgc cgtttcgaac gtcgtctcga acgtgcccgc
      721 ggtcatgatg ctgtcgccga tagcgtcatg cgccgccgat ccgcgctcgg cctggctcct
      781 cctcgcgatg gcgagcacct tcgccggcaa cctgaccctg attggctccg tggccaacct
      841 gatcgtcgcc gagggcgcgg ctagccgcgg cgcgaggctc ggcttcgtcg actatctcaa
      901 agccggcctg cccgtgacgc ttataacgct ggccatcggc acggcttggc tcgccatgac
      961 cgccagttga gatggctcgc tgttgaccag gaggctcggg cggacgtaca gtagccctac
     1021 cggggtgtcg ggagtttccg ttggaagcgc tcccgtctga gaacagaccc gatgaacctg
     1081 aaccgggtaa taccggcgga gggagtacta tgctcactca tacccatggc gcgagggttc
     1141 tcctcgcggc cgctgcgata tcgttcgcgg ccatcgccac ttcatgcgtc cagaaagacg
     1201 acgcccagct cgtcgtctac gcctacgact cgttcgcgag cgaatggggg ccggggccca
     1261 tcgttatccc ggccttcgag aaagccaccg gcatcacggt ggagctcgta gtgcccggcg
     1321 atgccggcca ggttttggcg cgcgccgtcg acgagaagcg ctcgccccgc gcggacgtca
     1381 ttgtcggcat tgacggcaac ctgttgccca aggccctggc cgccggcgtg cttgcggcct
     1441 acgagccgaa aggcagcgct tccgtgcctg cgcggctgag gctcgacgac gattggcgcc
     1501 tgacgcccta tgactggagc agcttctgcg tgatgtggga ctcggaaaag ctggccgtgc
     1561 cgccggcctc gctcgaggac ctgacgaagc ccgaatatgg ccgcaagctc atcctgatgg
     1621 atccgcgcac ctcgacgccc gggctcggct tcgtcgagtg gacggccgcg atctacggcg
     1681 acggactctc cggctattgg aagcgcctcg ctccgtccat cctgacgctg tcgcccggct
     1741 gggataccgg ttacgggctg ttcaccgccg gcgaggcgcc cctcgtcata tcgtatacga
     1801 ccagcgcggc ctaccacgcc gagtacgaga cggccggccg ttataaggcg ctcgagttcg
     1861 ccgacggcca ccctatccag gtcgaaggcg ccggcatcgt cgtcggcgcc aggcaccgcg
     1921 aggcggccga agcctttatc gatttcatgc tcggcgacga gttccaagcg gccctgcctt
     1981 tgaccaactg gatgtacccg gtcaatgagg gcgtcgtact gccgcccagc tacgaagcgt
     2041 ccaagaagcc ggccaagacc gtcagcgccg ggctagcgcc gaaccagctt gacgaagcgg
     2101 ttcgcacggc gctcgacgcg ctccaggggc gataggcccg ggccatgaag gcggtccgtt
     2161 cccggcgcgc ttcgctcccc gccgccgtac tgttcccggc gctcggagcg gcgctgctcg
     2221 tccccctcgg ggccgcgttc atgcccttgt tcgacggagg cgcgccggcc aacctggcgc
     2281 gagccttcgc cgatcctcgt ttctcgaggt cgttcgcgta cggtctgtcc caggccgccg
     2341 cgagcgccgc tttggcggcc gctatcggac tacccggggc cttcctgatc gctcggcgca
     2401 ccttccccgg taagaggctc ttaaccgccc tgtcggccgt ccccttctgc gtgcctccgc
     2461 tcataatcgc cataggcttc gtgctgtact acggccgcga aggcttcttg aaccgaacgc
     2521 tgatggcatc gttccgcctc gatcagccgc cgatcggttt tctgtactcg ttcgcgggcg
     2581 tcgtcatggc ccacggcttc tataatttcc cggtggtgat gcgcttcgtc ggcgatgcct
     2641 gggccaccgc gccgcgacgc cacgaggacg cggccaggct gctcggcgca tcggaacggc
     2701 gcgtcttcat gacggtgacg ctgccgtcca tcctgccgtc catcggcgcc tccatgtccc
     2761 tcgtcttcct catgtgcttc tatagcttcg tcatcgtgct gctgttcggg ggggcgggcg
     2821 tcgggacgcc cgaggtcgag ctgtatcggg ccgcccgctt cgagttcgac cgtccgctgg
     2881 cgagcgcctt cgcgctcgtc gagacggtcg tcgcgatggg cgcgctggcg ctgtacgccg
     2941 ccttcgagcg ccgggccgcg ggtgaccgcg aggagtcgga gcgcgtagcg ccgaacggct
     3001 tccgaagctc gggcggaatc gcgctcgccg tcctgtacgg ggccttcata gccgtctgtt
     3061 tcgtcggccc actggcctcg atagtcgcgg aatcgctcgc gatcaggaac gcctccaggg
     3121 tcggctcgag cctcggattg ggcaactacg tctcgctgtt cacgaggagg ggcttcgtcc
     3181 cggccctggc caacaccgcg ctcctggggc tcctctgcgc cgcgctcgcg agcgtggctg
     3241 gcttcacgct atccataggc ctcaagcgag cccgctc
//
LOCUS       DAOPHV010000277         3266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_40704_length_3266_cov_3.728434, whole genome shotgun sequence.
ACCESSION   DAOPHV010000277 DAOPHV010000000
VERSION     DAOPHV010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40704_length_3266_cov_3.728434"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..492)
                     /gene="rpsH"
                     /locus_tag="PLQ29_07955"
     CDS             complement(94..492)
                     /gene="rpsH"
                     /locus_tag="PLQ29_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPG86617.1"
                     /translation="MSVSDPISDMLTKVRNASMARHEKVDIPTSRLKLEIVKILKTEG
                     FIKNFKKIVQENQNFIRVFLKYDEENEPILHGIEKVSKPGRRVYAGYKGLPRVHNGYG
                     TLIVSTSEGVTTGKKAAEKKVGGELICTVW"
     gene            complement(503..688)
                     /locus_tag="PLQ29_07960"
     CDS             complement(503..688)
                     /locus_tag="PLQ29_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002557082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPG86618.1"
                     /translation="MARKAMILKALEKPKYMTRHVNRCKICGRPRGYLRKFDMCRICF
                     RKLASEGLIPGVTKSSW"
     gene            complement(700..1269)
                     /gene="rplE"
                     /locus_tag="PLQ29_07965"
     CDS             complement(700..1269)
                     /gene="rplE"
                     /locus_tag="PLQ29_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPG86619.1"
                     /translation="MAEKQQKNAYVPRLKKIYADKVASELKGEFGYTSTMQIPKVVKV
                     IVSMGVGEAKENKKILEAAVEDVGIITGQKAVKTKSRKSIATFKIREGQEIGCRVTLR
                     GAQMWEFLDRLLNVALPRVKDFRGVNPNAFDGHGNYSLGLTEQIIFPEIDYDKIEKVM
                     GMNIAIVTSARTDKEAKSLLGKLGMPFRK"
     gene            complement(1269..1592)
                     /gene="rplX"
                     /locus_tag="PLQ29_07970"
     CDS             complement(1269..1592)
                     /gene="rplX"
                     /locus_tag="PLQ29_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPG86620.1"
                     /translation="MNDVMIKTKLKKDDLVQVISGRERGKQGKIVKIDRDKGRAIVQG
                     VNMVKKAMKKKNQNDRGGIIEIEAPVRLCNMMIVCKKCGPTRVGFKLEGDAKKRVCRK
                     CGEAL"
     gene            complement(1603..1971)
                     /gene="rplN"
                     /locus_tag="PLQ29_07975"
     CDS             complement(1603..1971)
                     /gene="rplN"
                     /locus_tag="PLQ29_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPG86621.1"
                     /translation="MIQVESKLNVADNSGAKTVQCIKVLGGSKRRYASIGDIIVVAVK
                     AALPNSAIKKGTIEKAVIVRVHKEYRRPDGTYIRFDDNACVIIDANKNPKGKRIFGPV
                     ARELRDKDYMKIVSLAPEVL"
     gene            complement(1990..2253)
                     /gene="rpsQ"
                     /locus_tag="PLQ29_07980"
     CDS             complement(1990..2253)
                     /gene="rpsQ"
                     /locus_tag="PLQ29_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPG86622.1"
                     /translation="MEATVRKEKQVLQGVVVSDKMDKTIVVEIMMRKLHPLYKKYLTR
                     SKKVKAHDEKNDAKVGDTVRVVETRPLSRDKRWRLAEIVERAK"
     gene            complement(2266..2478)
                     /gene="rpmC"
                     /locus_tag="PLQ29_07985"
     CDS             complement(2266..2478)
                     /gene="rpmC"
                     /locus_tag="PLQ29_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPG86623.1"
                     /translation="MKNSFKELKLEELVAKRDELRKKYFDLRFQMVIGHVENPLQKRT
                     MRRQIARLNTLICQRERGASGAVAKA"
     gene            complement(2483..2905)
                     /gene="rplP"
                     /locus_tag="PLQ29_07990"
     CDS             complement(2483..2905)
                     /gene="rplP"
                     /locus_tag="PLQ29_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPG86624.1"
                     /translation="MLQPKRVKHRKVQRGRVKGNATAGNAISFGEFGLVCEEPMWITN
                     RQLEAARVALNRSIKRGGKLWVRVFPDKPFCKKPLEVRMGKGKGGPEYWVAVVRPGTV
                     MFELSGVPRDAAAEAMRLAGSKLPIKTKFVVRADMEME"
     gene            complement(2909..>3266)
                     /locus_tag="PLQ29_07995"
     CDS             complement(2909..>3266)
                     /locus_tag="PLQ29_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPG86625.1"
                     /translation="GTAMKAGAQGCKIRMSGRLGGADMSRTEEYKEGRVPLHTLRADI
                     DYGFWEANTTYGKIGVKVWICKSDNAMMGEKKDDAGLMPGRPRREHGDRPERGGQRGS
                     RGPNPGYSRGPREARS"
BASE COUNT          520 a         1069 c          883 g          794 t
ORIGIN      
        1 cacgttgacc ttgtttccct gaagggcaac cttgacgcct ttgggtactg taatcggctt
       61 atagcctatc ctcgacatgg cggttcccct cttttaccag acggtgcaga tgagttcgcc
      121 gccgaccttc ttctcggcgg ccttcttacc ggtggtcacg ccctctgagg tcgagacgat
      181 aagggttccg tagccgttat gcacccgcgg cagacccttg tagccggcat agacccttcg
      241 gccgggcttg gagaccttct cgataccgtg gagaatcggt tcattctcct cgtcgtactt
      301 gaggaagacc cggataaaat tctggttctc ctgcacgatc ttcttgaagt tcttgatgaa
      361 gccctcggtc ttgaggatct ttacgatctc gagcttaagc cgcgaggtcg ggatatctac
      421 cttctcgtgg cgagccatgc tcgcgttgcg gaccttggtc agcatatcgg agatgggatc
      481 ggaaacgctc attccagtcc tcctaccagc tcgatttggt tacgcccgga atgaggccct
      541 cgctcgcgag cttccggaaa caaatacggc acatgtcgaa cttccggaga tacccccggg
      601 gccgaccgca tatcttgcat cgattcacgt gcctggtcat gtacttcggc ttctcgagcg
      661 ccttcagtat catagctttc ctggccattc gattctcccc tacttcctga acggcatgcc
      721 cagcttgcca aggaggctct tggcttcctt gtcggtgcgg gcgctcgtaa cgatggcgat
      781 gttcataccc atcaccttct cgatcttatc gtaatcgatc tccgggaaga tgatctgctc
      841 ggtaaggccc agcgagtagt tcccgtggcc gtcgaaggcg ttcgggttaa ctccacggaa
      901 atccttgacg cgcggcagag cgacgttcag gagcctatca aggaactccc acatctgcgc
      961 cccgcggagc gtgaccctgc agcctatctc ctggccctct cgaatcttga aggtcgcgat
     1021 cgacttcctc gacttcgtct taacggcctt ctggccggtg atgatgccga cgtcctcgac
     1081 agcggcctcg aggatcttct tgttctcctt ggcctctccg acgcccatcg acacgatgac
     1141 cttgaccact ttcgggatct gcatcgtcga ggtatagccg aattcaccct tgagttcgga
     1201 cgccacttta tcggcgtaga tcttcttgag cctcggaacg tacgcgttct tctgctgctt
     1261 ctcggccatc agagcgcctc cccgcacttc cggcagacgc gcttcttcgc gtcgccctcg
     1321 agcttaaacc caacgcgggt aggtccgcac ttcttgcaga cgatcatcat attgcagagg
     1381 cgaacgggcg cctcgatctc gatgatccct ccgcgatcgt tctggttctt cttcttcatg
     1441 gccttcttga ccatgttgac gccctgaacg atcgcccttc ccttgtcgcg atcgatcttg
     1501 acgatctttc cctgcttgcc gcgctccctg ccggagatga cctgcacgag atcgtccttc
     1561 ttcagcttcg tctttatcat gacgtcattc atcgcccgct ccttagagga cctcgggggc
     1621 cagcgacacg atcttcatgt agtccttgtc gcgcagctcc ctggcgacgg gaccgaagat
     1681 gcgcttgccc ttcgggttct tgttcgcgtc aatgatgacg caggcgttat cgtcgaacct
     1741 gatgtaggta ccgtcaggcc tgcggtactc cttgtgtacg cgcacgatga cggccttctc
     1801 tatggttccc ttcttgatgg cggaattagg cagggccgcc ttgacggcca cgacaataat
     1861 atcgccgatg gatgcgtaac gccgcttcga accgccgagc accttgatgc attgcaccgt
     1921 cttggcgccg gagttgtccg cgacgttcag cttggattca acctgtatca taccggtcct
     1981 ctccgccgct tacttcgcgc gctcgactat ttcggccagg cgccagcgct tatcgcgcga
     2041 cagcggccga gtctccacga ctctaacggt atcgccgacc ttggcgtcgt tcttctcgtc
     2101 gtgtgccttt accttcttgc tccgggtcaa gtacttctta tagagcgggt gcagcttacg
     2161 catcatgatc tcgacgacga tggtcttgtc catcttgtcg ctgaccacca cgccctgcag
     2221 gacctgtttc tctttcctaa ccgttgcttc catgtccggc ctccgttaag ccttggcgac
     2281 tgcgcccgag gcgccgcgct cgcgctggca gatgagggta ttgagcctcg cgatctgcct
     2341 gcgcatcgtc cgcttctgga gcgggttctc tacatggcct atgaccatct ggaagcgcag
     2401 gtcgaagtac ttcttcctga gctcgtcgcg cttagccacg agttcctcga gcttcagctc
     2461 cttgaacgag ttcttcatgc gatcactcca tctccatgtc cgcgcggacg acgaacttgg
     2521 tcttgatcgg cagcttgctg ccggcgaggc gcattgcctc ggcggcggcg tcccgcggga
     2581 cgccggacag ctcgaacatg accgtgcccg gcctgaccac ggctacccag tactccgggc
     2641 cgcccttacc cttgcccatg cggacctcga gcggcttctt gcagaacggc ttgtcgggga
     2701 agaccctgac ccacagcttg ccgccgcgct tgatggaccg gttcaaggcc acacgggcgg
     2761 cctcgagctg gcgattggtg atccacatcg gctcctcgca aacgaggccg aactcgccga
     2821 acgatatggc gttacccgcg gtggcgttgc cctttaccct accgcgctgc accttgcggt
     2881 gcttgactct cttaggctgc aacatttcct agctcctcgc ctcgcgcggc ccacggctgt
     2941 agcccgggtt gggcccacga gatccgcgct gtccgccgcg ctcgggcctg tcaccgtgct
     3001 cgcggcgcgg acggccgggc atcaggccgg catcgtcctt cttttcgccc atcatcgcgt
     3061 tgtcgctctt gcagatccac accttgacgc cgatcttgcc gtaggtggtg ttggcctccc
     3121 agaagccgta gtcgatgtcg gccctgaggg tatgaagcgg cacgcggcct tccttatact
     3181 cttcggtgcg actcatgtcg gcgccgccga gacggccgga catgcggatc ttgcagccct
     3241 gggcgcccgc cttcatcgcg gtgccg
//
LOCUS       DAOPHV010000278         3258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_40835_length_3258_cov_3.987200, whole genome shotgun sequence.
ACCESSION   DAOPHV010000278 DAOPHV010000000
VERSION     DAOPHV010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40835_length_3258_cov_3.987200"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLQ29_08000"
     CDS             <1..234
                     /locus_tag="PLQ29_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86626.1"
                     /translation="DRVLEGSLWSLDRAISAHGASAAATRPASNKASNKASNKASNKA
                     SNKASNKASNKASSKAAGEDVPTGLPTDTNSEE"
     gene            238..741
                     /locus_tag="PLQ29_08005"
     CDS             238..741
                     /locus_tag="PLQ29_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HPG86627.1"
                     /translation="MAFYEISPRAVEGNVFDRVQKTWMLVTAGTKASWNTMTANWGGF
                     GHLWNRDVAFVFVRPTRYTYAFIERTGLLTLSFFGEEWRDALTVCGTMSGRDVDKAGL
                     TGLKPAEPRPGFIGFEQADLTFACRVIHKQDIDPAGFVDGSLEGHYDGDYHRVYVAEI
                     VAALARA"
     gene            747..>3258
                     /locus_tag="PLQ29_08010"
     CDS             747..>3258
                     /locus_tag="PLQ29_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPG86628.1"
                     /translation="MRSRLPEELLGELDDPSARFVFPSEIAAASALTAALAATGRRAL
                     PSRRFLGWDAFKSEVFAGDAEGRPSTKAVRAVFARALAAENARSPFLRSVIPAQSAAS
                     SLRFARSMAAALPALRAVPDGPGDHLADWREIRRRYELFMGARGLYEAAWLGRSASRP
                     AARWVLLYPDLTEDWDDYAEAVLALPGSSAILSDRLGAEPVRAARFGTAVEEIRAVLL
                     AIRDEVASGADPAGIVISAAAPESTLPLLEREAAVAGVPLDVREGRPLSESSGGRLLA
                     DAKALADSGMSFEALRRLLLDASIPWKEAEAARRLLAIGVRAHIVAPLPGGRDVWEAS
                     IGDDEPARRLYRGLRTASSRIAGSTDFRSLRAAFDAFKRAFLDEALWSPRQDDEMARC
                     LAALDELDEAASTAGLERVPGAVDAWLEQLSETRYVPASTTGGIAVYRFPVAAGAMPD
                     LHFVVNLAQGAAVAASRPLAFMRADERARLGAEDRDLSAGLVRLLARSGGRVFMSYSE
                     DGPDGVRPPHPAVYAVPPESSGLVYERDRWLPNLEADGQAIAEAFPTQAACARAAAVT
                     VVSPSSPDWSGATPDAPATMGRWATEAVRASMSRDGRLRLSATAIEGYASCAFRRVFA
                     RTLKVEAMESGLSFIDSLLVGGIYHDAFGRLFRPLAAAGRTIVAPEAAGEEARPSGAD
                     VDAAIAAAITGAGAELGPMAAVLVSTAKPALRRDFARAVAALVPALDGLVPTMVDDED
                     LIAALRGVDADLYGRPDLVCLPPGTAPVADRLKAVIVDYKKSKIPKRAELQPAEDGSV
                     GAIQIPAYTVLIRAAGLEPFAAYYLSVEKAGEGGKGLLE"
BASE COUNT          464 a         1190 c         1113 g          491 t
ORIGIN      
        1 gaccgggtgc tcgagggctc gctctggagc ctggaccgcg ctataagcgc ccatggggca
       61 tcggccgcgg ctacgcgccc ggcttctaac aaggcttcta acaaggcttc taacaaggct
      121 tcgaataagg cttcgaataa ggcttcgaat aaggcttcga ataaggcctc gagcaaggcg
      181 gcgggcgaag acgttcccac ggggctgccg acagacacga acagcgagga gtagcgtatg
      241 gcattctacg agatcagccc gcgggccgtc gaaggcaacg tgttcgaccg cgtccagaag
      301 acctggatgc tcgtcacggc cgggacgaag gcttcctgga acacgatgac cgccaactgg
      361 ggcgggttcg gccacctgtg gaaccgcgat gtcgccttcg tattcgtgcg ccctacgcgc
      421 tacacctatg cgttcatcga aaggaccggc ctcctgaccc tgtcgttctt cggggaggaa
      481 tggcgcgacg cgctgaccgt ctgcggcacg atgtctggcc gcgacgtcga caaggccggt
      541 ttgaccgggc tcaagccggc cgagcctcgg ccaggcttca taggtttcga acaggccgac
      601 ctgaccttcg cctgccgcgt gatccataag caggacatcg acccggccgg cttcgtcgac
      661 ggctcgctcg aggggcacta cgacggcgat taccaccggg tctacgtcgc ggagatcgtc
      721 gcggccctgg cccgcgcgta gagcccgtgc ggagccgact gcccgaggag ttgctcggcg
      781 agctcgatga cccgagcgcc aggttcgtct tcccgagcga gatagccgcc gcgtcggcgc
      841 tgaccgcggc cctggccgcc accgggagga gggcgctgcc gtcccggcgc tttctcggat
      901 gggacgcgtt caagtccgag gtgttcgccg gcgacgctga gggtcggccg tcgaccaagg
      961 ccgtgcgcgc cgtcttcgcg cgggcgctcg cggccgaaaa cgctcgctcg cccttcctac
     1021 gctccgtcat acccgcccag tcggccgcct cgtcgctcag gttcgcgcgc tccatggcgg
     1081 cggctctccc agccctacgc gccgtgccgg acggtccggg cgaccatctg gccgactggc
     1141 gagagatacg ccgacgctac gagctgttca tgggcgcgcg gggcctctac gaggccgcct
     1201 ggctcggccg ctccgcatcg cgtcccgcag cccgctgggt gctcctgtac ccggacctga
     1261 ccgaggactg ggacgactac gccgaggccg tactcgccct gccaggctca tccgccattc
     1321 tgtccgaccg cctcggcgca gagccggtcc gcgccgcgag gttcggcacc gcggtcgagg
     1381 agatccgcgc cgtgctactc gccatacgcg acgaggtcgc ctccggcgcc gacccggccg
     1441 gcatcgtcat cagcgccgcc gcgccagaat cgaccctgcc cctgcttgaa cgcgaggccg
     1501 cggtcgccgg agtgccgctc gatgtgcgcg agggccggcc gctgtccgag agctcgggcg
     1561 gccggctact cgccgacgcc aaggccttgg ccgacagcgg catgtctttc gaggcgctca
     1621 gacgccttct cctcgacgcc tccatcccct ggaaggaggc cgaggccgcc cgcaggctgc
     1681 tcgccatagg cgtacgagcc catatcgtcg cgccgctccc cggagggcgc gacgtctggg
     1741 aggcctcgat aggcgacgac gagccggcgc ggcgcctgta ccgcggcctc aggaccgcca
     1801 gttcgaggat agcgggctcg accgacttcc gatccctccg cgcggcgttc gacgcgttca
     1861 agcgcgcctt cttagacgag gcgctctggt cgccgcgcca ggacgacgag atggcccggt
     1921 gtctggccgc gctcgacgag ctcgacgagg cggcgtcaac cgccgggctc gagcgcgtac
     1981 cgggcgccgt ggacgcgtgg ctcgagcagc tttccgagac gcgctacgtg ccggcatcca
     2041 ccacgggcgg tatcgcggtg taccgcttcc cggtggccgc cggcgcgatg cccgacctgc
     2101 acttcgtcgt caacctggcc cagggagcgg ccgtcgccgc ctcgaggccg ctagcgttca
     2161 tgcgggccga cgaacgggcc aggctgggcg cggaggaccg cgacctgtca gccggactcg
     2221 tgcggctcct ggcccgatcc ggcggccgcg tcttcatgag ctactcggaa gacgggccgg
     2281 acggtgtgcg gccgccccac cccgccgtct acgccgtccc gcccgagtcg tcgggcctcg
     2341 tctacgaacg cgaccgctgg ctaccgaacc tcgaggccga cggccaggcg atagccgagg
     2401 ccttcccgac ccaggcggcc tgcgctcggg cggcggccgt caccgtcgtt tccccgtcct
     2461 cgccggactg gtccggagcg acgcccgacg ccccggcgac gatgggccgg tgggccaccg
     2521 aggccgtccg tgcctccatg agccgcgacg gccgcctcag gctatcggcc acggcgatcg
     2581 agggctacgc ctcctgcgcc ttccgccggg tcttcgcgcg gacgctgaag gtcgaagcta
     2641 tggaatccgg cctgtcgttt atcgacagct tgctcgtcgg cggcatctac catgacgcgt
     2701 tcggccgcct tttcaggccg ctggcggcgg ccggccgcac gatcgtcgcg ccggaagccg
     2761 ccggcgagga agctcgtccc tcgggcgccg acgtcgacgc tgccatagcc gccgccataa
     2821 ccggcgccgg cgccgagctc gggccgatgg ccgccgtgct cgtatcgacc gcgaagccgg
     2881 ctctgcgccg ggacttcgct cgcgcggtcg ccgccctcgt cccggccctg gatggcctcg
     2941 tgccgaccat ggtcgacgac gaggacctga tcgcagcgct acggggcgtc gacgccgacc
     3001 tctatggtcg tccggacctc gtctgcctgc cgcccggtac agccccggtc gccgatcgac
     3061 tcaaggccgt catcgtcgac tacaagaagt ccaagatccc gaagcgggcc gagctacagc
     3121 cggccgagga cggctcggtg ggcgccatcc agataccggc ctatacggtg ctcatacgcg
     3181 cggcgggcct cgagcctttc gcggcgtact acctgtccgt cgagaaggcc ggcgagggcg
     3241 gcaaggggct gctcgagg
//
LOCUS       DAOPHV010000279         3257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_40849_length_3257_cov_4.730481, whole genome shotgun sequence.
ACCESSION   DAOPHV010000279 DAOPHV010000000
VERSION     DAOPHV010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40849_length_3257_cov_4.730481"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PLQ29_08015"
     CDS             complement(<1..507)
                     /locus_tag="PLQ29_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPG86629.1"
                     /translation="MEYRRLGSAGVKVSALSLGSWVTYGSQVDVAAAAEMMAAAYDRG
                     VNFFDNAENYAAGKSEEIMGAALKRLGWRRASYLVSTKFYWGLAEGPNEKNTLNRKYL
                     MQAIDGSLARLGLDYVDLVYCHRPDPDTPMEETVWAMSDIVASGRALYWGTSEWSAEQ
                     IRQAWEIAD"
     gene            complement(753..2162)
                     /locus_tag="PLQ29_08020"
     CDS             complement(753..2162)
                     /locus_tag="PLQ29_08020"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPG86630.1"
                     /translation="MRSTIGPSRHASGIGIALFAACAALLAGSCGGGGSREPAAGQAP
                     AEAAQPSESRAAEASAPDAGAPSDAAQAGRPAPDAAAGAAATGTATEATPGEPAATEG
                     AGAAQATGSEPSRSAPRRLGELVFTEGAVTLHRAGSSPRRADIGDVVAAYDLIATGPG
                     ARAEVDIGSGAAGGATVKLTENTAFYFDTKELDDAARRTVLQLLSGAVAIKVDRLATG
                     SFSVTTDSAVLGVRGTVFIVDTVPDGSMLVSCDTGAVSVRSDGAVTTAKPGGVVELTD
                     AGGLRLLAVQPEALADYRGAWRDEAYKGFAKRALSYTSAYAKTLDGGAPTLAAAVSRL
                     RAQQPALDAWRAARAAGRVPRFTDWTAEKKAVGAALFDCLKAAFAVERPYYRLLELEA
                     LHDAGVGVGALSDGRKSSSFFGSFGKDYAGLAQDLAAVREALALFSWAAAGSPLGDFF
                     GAKAEALGTGAALFEDEDW"
     gene            complement(2159..>3257)
                     /locus_tag="PLQ29_08025"
     CDS             complement(2159..>3257)
                     /locus_tag="PLQ29_08025"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG86631.1"
                     /translation="LYNADPDFQKLFKVCAEFDSTMPRNDDGLREYVSFARKITEEEG
                     LLEPSADGLAAVAEYGVRLSGSRDRLSTRFSKVADLLREADYRASRSGKDAIDAESVR
                     EAERVRSWLAALPEEKVTEMIVSGEILLEAEGSRVGRVNGLAVHDRGYYAFGLPAVIS
                     AQVSPGESGVINIEGESGLSGEIYDKAVLIVEGFLRSRYARDFPLAVSASICFEQSYT
                     AIEGDSASSTAVYALLSAIAGVPLRQDIAVTGSVNQMGQIQPVGGVAEKVEGFYRICL
                     RAGLSGTQGVMIPKQNVVNLTLSKEVLDAVEAGRFSIYAVSTIDEGIEVLADRAPGAP
                     DALGRFPKGSFNGLVADELRRMAETIKEYRE"
BASE COUNT          474 a         1117 c         1174 g          492 t
ORIGIN      
        1 gtcggctatc tcccaggcct ggcgtatctg ctcggcgctc cactcggagg tgccccagta
       61 cagggcgcgg ccggaggcga cgatgtcgct catcgcccag acggtctcct ccatcggcgt
      121 gtcgggatcg gggcggtggc agtagaccag gtcgacgtag tcgaggccga gcctggcgag
      181 cgagccgtcg atcgcctgca tcaggtactt gcggttgagc gtgttcttct cgttcgggcc
      241 ttcggcgagg ccccagtaga acttggtcga cacgaggtag ctcgcccgac gccagccgag
      301 ccgcttgagg gccgccccca tgatctcctc ggacttgccc gcggcgtagt tctccgcgtt
      361 gtcgaagaag ttgacgccgc ggtcgtaggc cgccgccatc atctcggccg ctgcggctac
      421 gtccacctgc gagccatagg taacccatga tccgagcgac agcgccgaga ccttgacgcc
      481 tgcgctaccg agcctgcggt attccatcgt tgctccttga cggtcacgtg gaccgggttt
      541 aaggataccg ctccgggcga ggcgtcggac agttcgcttc gagtcctttc gaattaaaaa
      601 gcgcggcctc ccggccgcgc gacgatctac catcgataag ggcgccgtcc ggcgcctcgt
      661 cccggctacg ccgggacgct attccgtccg acgccggcca gcccgagccg ttatcgggcg
      721 ggcagccccg agagccgcat cctgggccgg gcttaccagt cttcgtcctc gaacaaggcg
      781 gcgccggtac ccagggcctc ggccttggcg ccgaagaagt cgccgagcgg gctgcccgcc
      841 gcggcccatg agaacagcgc cagggcttcg cgtaccgccg ccaggtcctg ggcgaggccg
      901 gcgtagtcct tgccgaaaga gccgaagaac gacgagctct tgcgaccgtc ggagagggcg
      961 ccgacgccga cgcccgcgtc gtggagggcc tcgagctcga gcaggcggta gtaggggcgc
     1021 tcgaccgcga aggcggcctt gaggcagtcg aacagggccg cgcccacggc cttcttctcg
     1081 gcggtccagt cggtaaagcg cggtacgcgg ccggcggcgc gggcggcgcg ccacgcgtcc
     1141 agggccggct gctgcgcgcg tagcctggag acggccgcgg cgagggtcgg ggcgcccccg
     1201 tcgagggtct tggcgtaggc gctggtgtac gatagcgcgc gcttggcgaa gcccttgtaa
     1261 gcctcgtcgc gccaagcgcc gcgataatcc gccaaggcct ccggctggac ggcgagcaac
     1321 ctgaggccgc ccgcgtccgt cagctcgacc acgccgcctg gtttggcggt ggtcacggcg
     1381 ccgtcggagc gcaccgatac ggcgcccgtg tcgcaggaga cgagcatgga gccgtcgggt
     1441 acggtgtcga cgatgaagac ggtgccgcgc acgccgagca ctgccgagtc ggtggtaacg
     1501 ctgaaggagc cggtggccag ccgatccacc ttgatcgcca cggcgccgga tagcagctgt
     1561 agcacggtgc gcctggccgc gtcgtcgagc tccttcgtat cgaagtagaa ggccgtattc
     1621 tccgtcagct tgacggtggc gccgccggcc gcgccggagc ctatatccac ctcggcgcgg
     1681 gcgccggggc cggtcgcgat caggtcgtag gccgccacca cgtcgccgat gtcggcgcgc
     1741 cgcggcgacg agccggcgcg gtggagcgtc acggcgccct cggtgaagac gagctcgccc
     1801 aggcgccgcg gcgccgaccg ggacggttca gaacccgtcg cctgggccgc gccggcgccc
     1861 tcggtcgcgg cgggctcgcc gggagtcgcc tcggtagcgg tcccggtggc tgcggcgcct
     1921 gcggcggcgt ccggggccgg acggccggct tgggccgcat cgctcggggc gccggcgtcg
     1981 ggcgctgatg cctcggcggc tcgggactcc gatggctggg cggcctcggc tggagcctgg
     2041 ccggcggccg gctcgcgcga gccgccccct ccgcacgagc cggctagaag cgccgcgcag
     2101 gcggcgaaca gcgcgatacc aataccgctc gcgtgacgcg aaggcccaat agtcgatctc
     2161 attcgcgata ttcctttatg gtctccgcca tgcgtctgag ctcatcggcc acgaggccgt
     2221 tgaagctacc cttgggaaag cggccgagcg cgtccggggc tccgggggcc cggtcggcga
     2281 gcacctcgat gccctcgtcg atggtggaga cggcgtagat ggagaagcgc ccggcctcga
     2341 cggcgtcgag cacctccttc gaaagcgtca ggttgacgac gttctgcttg ggtatcatca
     2401 cgccctgggt gccggacagc ccggcgcgca ggcagatgcg gtagaagccc tcgactttct
     2461 ccgcgacgcc gccgaccggc tggatctggc ccatctggtt gaccgagccg gtcacggcga
     2521 tgtcctggcg cagcggcacg ccggctatgg ccgacaggag ggcgtagacg gcggtcgagg
     2581 aggcggagtc gccctcgatg gcggtgtacg actgctcgaa gcagatgctc gccgataccg
     2641 cgagcgggaa gtccctggcg tagcggctac gcaggaagcc ttcgacgatc agcaccgcct
     2701 tgtcgtagat ctcgccggac agccccgact cgccctcgat gttgatgacg ccagactcgc
     2761 ccggcgagac ctgggccgag atcacggcgg gcaggccgaa ggcgtaatag ccgcggtcgt
     2821 ggacggccag gccattgacg cggcctaccc gggagccctc ggcctcgagg agtatctcgc
     2881 cggagactat catctcggtg accttctcct cgggcagggc ggccagccag gaacgcacgc
     2941 gctcggcctc gcggaccgac tcggcgtcga tcgcgtcctt gccggagcgg gaggcccggt
     3001 agtcggcctc gcgcaggagg tccgccacct tggagaaacg cgtcgacagc cggtcgcggc
     3061 tacccgacag ccggacgccg tactcggcca cggcggccag gccgtcggcc gacggctcga
     3121 ggaggccctc ctcctcggtg atcttgcgcg cgaacgatac gtactcgcgt agcccgtcgt
     3181 cgtttcgcgg catggtggag tcgaactcgg cgcagacctt gaacagcttc tggaagtcgg
     3241 gatcggcgtt gtacagg
//
LOCUS       DAOPHV010000280         3257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_40850_length_3257_cov_4.377264, whole genome shotgun sequence.
ACCESSION   DAOPHV010000280 DAOPHV010000000
VERSION     DAOPHV010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40850_length_3257_cov_4.377264"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="PLQ29_08030"
     CDS             complement(<1..1095)
                     /locus_tag="PLQ29_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HPG86632.1"
                     /translation="MEEAGYSLGIDSSTQGTTAVIVDLGSRSIAARAKVRYRDDPRLS
                     AFALDRAAPILPPREEGEADQPVGVFLAALDAVLSDLPGRMLAGVRAVAVSAQQHGQV
                     WLSAGAPSAFADLRAAGSGAAGRPALAERLASSLASDRSPIWMSSNTASEADHLRKAA
                     GGTVAMTALSGSDSPLRFSGAVLRHRALREPELYAACRTVHLISSFLAGVFSGRADVP
                     IDWGNGAGTSLMDWEGRRWSDGIVAAAADGLPGGAAALSARLPELSHPLSRVGSVARY
                     FTERYGIPADAIVVAGSGDNPQTKVLAGGGLLSLGTSFVLMTGGRAPHPAANAMYDGL
                     GRPFLFGCRTNGALTWEAARLERGVGADDFA"
     gene            complement(1126..2448)
                     /gene="xylA"
                     /locus_tag="PLQ29_08035"
     CDS             complement(1126..2448)
                     /gene="xylA"
                     /locus_tag="PLQ29_08035"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HPG86633.1"
                     /translation="MKELFPTVPKIDYDGPDSNKELAFKYYRPDAAAAGSSAGITQKD
                     SLRFSMAFWHSMTAGGRDPFGDPTAVRPWDGVNDSMEVARLRMRGVFELTEKLSIPFF
                     CFHDRDIAPQGDTLRETNRNLDKIVALAKDLMRSSPTRLLWGTANLFGDPRFMHGAAT
                     SPDPRVFAYAAGQVKKALEAALELGGLNYVFWGGREGYETLLNTDMKREQDNLARFLR
                     MAVEYAKEIGFRGQFLIEPKPKEPTKHQYDADAAACYAFLKEYELLPYFKLNIETNHA
                     TLAGHTIQHELRYARERGILGSVDANQGDELLGWDTDQFPTNLYVSTLMMYEILKNGG
                     LGSGGLNFDAKPRRGSYRPEDLASAHIAGMDAMARGYEAAARMLADGALEKPLAERYA
                     GWDEGLGASIEAGKESLRSLEAASLDIKAIELSSGRQEYLESVVNRYI"
     gene            2586..>3257
                     /locus_tag="PLQ29_08040"
     CDS             2586..>3257
                     /locus_tag="PLQ29_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HPG86634.1"
                     /translation="MRFDDIVFVYHLQDERQIAWHGRRHSHSGGGYEFHYFISGDGSF
                     KNGTATWSIRPGSLHLTPPGCVHQILATNLRKPITYFAVLFSSDGDAELERLLGRAIG
                     SLARPTDIGTSHRFFFADLMERNSSGQVDLVEAARHSFIAFVYELLAGAPSAYGSAEN
                     AHVEKAIAIMQANIEGNIDLDALCGRLGLSCEHFVRLFGQRMRMPPMRYYARLKIEAA
                     RAMLSS"
BASE COUNT          490 a         1092 c         1111 g          564 t
ORIGIN      
        1 ggcgaagtcg tcggccccga cgccgcgctc gagccgcgcc gcctcccagg ttagcgcgcc
       61 gttggtacgg cagccgaata ggaatggccg gccgaggccg tcgtacatcg cgttggcggc
      121 cgggtgcggc gcgcggccgc cggtcatcag cacgaagctc gtgcccaggg ataggagccc
      181 gccgcccgcc agtaccttgg tctgcggatt gtcaccggaa ccggcgacga cgatagcgtc
      241 cgccgggatg ccgtagcgtt ccgtaaaata gcgggcgacc gaaccgacgc gcgacagcgg
      301 gtgggatagc tccggcaggc gggcggacag ggctgcggcg ccgccgggca ggccgtcggc
      361 ggccgcggcg acgatgccgt cgctccatcg ccgtccttcc cagtccatca ggctggtgcc
      421 ggcgccgttg ccccagtcta taggaacgtc ggctcggccc gagaataccc ccgcgaggaa
      481 cgacgatatc aggtggacgg tacggcaggc cgcgtagagc tccggctcgc gcaaggcccg
      541 atgccgtagc acggcgccgg agaaccggag cgggctgtcg gagccggaca gggcggtcat
      601 cgccacggtc ccgccggccg ccttgcgcag atggtccgcc tcgctcgcgg tgttggagct
      661 catccagatg ggcgaccggt cgctcgcgag cgacgaggcg agccgctccg ccagagcggg
      721 ccgccccgcc gcgcctgagc ctgcagcccg gaggtcggcg aacgccgacg gggcgccggc
      781 ggacagccag acctggccgt gctgctgggc cgatacggct acggcgcgta cgccggcgag
      841 catccgccca ggcaggtcgg acaggacggc gtcgagggcc gcgagaaaga ccccgaccgg
      901 ctggtcggcc tcgccttcct ctcggggcgg caggatgggt gcggctcgat ccagggcgaa
      961 cgcgctcagc ctgggatcgt cgcggtagcg taccttggcc cgagccgcga tcgatcgcga
     1021 gcccaggtcg acgatgacgg ccgtggtgcc ctgggtgctc gagtctatcc cgagcgagta
     1081 tcccgcctct tccatccttc gtcctcctct cgactgggcc gcggcctaga tgtagcggtt
     1141 gacgaccgac tcgaggtact cctgccgccc cgaggagagt tcgatcgcct tgatgtcgag
     1201 cgaggcggcc tcgaggctcc ggaggctctc cttgccagcc tcgatggagg cgcccaggcc
     1261 ctcatcccag ccggcgtaac gctccgcgag cggtttctcg agcgccccat cggcgagcat
     1321 gcgggccgcc gcctcgtagc cgcgggccat cgcgtccatg ccggctatgt gggctgacgc
     1381 caggtcctcg ggcctatagc tgccgcgcct gggcttggcg tcgaagttga ggccgcccga
     1441 gccgaggccg ccgttcttga gtatctcgta catcatcagc gtcgagacgt ataggttcgt
     1501 cgggaactgg tcggtgtccc agcccaggag ctcgtcgccc tggttggcgt cgaccgagcc
     1561 gagtatgccg cgctcgcggg cgtagcgcag ctcgtgttgg atggtgtgcc cggcgagggt
     1621 ggcgtggttc gtctcgatgt tgagcttgaa gtagggcagg agctcgtatt ccttgaggaa
     1681 ggcgtagcag gcggcggcgt cggcgtcgta ctggtgcttc gtcggctcct tgggcttggg
     1741 ctcgatgagg aactggcccc tgaagcctat ctccttggcg tactcgacgg ccatgcgcag
     1801 gaaccgcgcc aggttgtcct gctcccgctt catgtcggtg ttgagcagcg tctcgtagcc
     1861 ctcgcgcccg ccccagaaga cgtagttcag gccgccgagc tccagcgccg cctcgagcgc
     1921 tttctttacc tggccggcgg cgtaggcgaa gacccgcggg tcggggctcg tcgccgctcc
     1981 gtgcatgaag cgcgggtcgc cgaacaggtt ggccgtgccc cagagcagac gggtagggct
     2041 cgagcgcatc agatccttgg ccagggcgac tatcttgtcg aggttgcggt tcgtctcgcg
     2101 gagcgtgtcg ccctgcggcg cgatgtcgcg gtcgtggaag cagaagaacg gtatggacag
     2161 cttctcggtc agctcgaaca cgccgcgcat ccgcagcctc gctacctcca tcgagtcgtt
     2221 cactccgtcc caggggcgca ccgccgtagg gtcgccgaag ggatctcggc cgcccgccgt
     2281 catcgagtgc cagaacgcca tcgagaagcg caggctgtct ttctgggtga ttccggcgga
     2341 cgagcccgcg gccgcggcgt ccggcctata gtatttaaag gccagctcct tgttcgagtc
     2401 ggggccatcg tagtcgatct tgggtactgt cgggaaaagc tctttcacgg aatcctcctc
     2461 gcgctgcttg cctgatccgt cgattctagg ccgccgatca agggaatcac agcgccgaat
     2521 ttgactctac atacctaaaa ttgatattcc gtgctatcgt cgatgagggc ttcgggaggt
     2581 gcggcatgcg cttcgacgat atagtcttcg tctaccatct ccaggacgag cggcagatag
     2641 cctggcacgg ccgtcgccac tcgcactcgg gcggcggcta cgagttccac tacttcatct
     2701 ccggcgacgg tagcttcaag aacggcacgg ctacctggtc gatacgcccg ggcagcctcc
     2761 acctcacccc tcccggatgc gtccatcaga tcctggctac caacctgcgc aagccgataa
     2821 cctacttcgc cgtgctcttc agctccgacg gtgacgccga gctcgagcgg ctgctcgggc
     2881 gagcgatcgg gtcgctggcc cggcctaccg atatcggcac ctcgcatcgc ttcttcttcg
     2941 ccgaccttat ggaacgcaac tcgtcgggcc aggtggacct cgtcgaggcc gctcggcact
     3001 cgttcatcgc cttcgtgtac gagctgctcg ccggcgcccc gtcggcctac ggctcggcgg
     3061 agaacgccca cgtggagaag gccatagcga tcatgcaggc gaatatagaa gggaacatcg
     3121 atctggacgc gctctgcgga cggctcggcc tgtcctgcga gcacttcgtg cggctcttcg
     3181 ggcagcgcat gcgcatgcct ccgatgcgct actacgcgag gctcaagatc gaggccgcgc
     3241 gcgcgatgtt gtcgagc
//
LOCUS       DAOPHV010000281         3255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_40879_length_3255_cov_4.177812, whole genome shotgun sequence.
ACCESSION   DAOPHV010000281 DAOPHV010000000
VERSION     DAOPHV010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40879_length_3255_cov_4.177812"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PLQ29_08045"
     CDS             complement(<1..916)
                     /locus_tag="PLQ29_08045"
                     /inference="COORDINATES: protein motif:HMM:NF015791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HPG86635.1"
                     /translation="MRPLVGFASALALDASARDGLGDDLLMEAAAVGMAAAIDADPAL
                     RKAAASGSPSIAAVCGGGNNAGDALAVLRRLAFSGWKDLVALVPDSLGPLASRRLAEA
                     GLAGARVIGLGDDAAHEAVARAGVVLDGLSGVGFKGPARAALVQAAALIASARGPVVS
                     MDVPSGVGALGSVAADPSAPVCADITLCVEPLKAELYYPGFRPYAGRILPIGGVFDRS
                     AGAASTMVLLEPGDLEDALPGLDPDCHKGDRGAVCAFAGSYGSTGAAVLCSRAAAAAG
                     AGSVTLLARDELVPMLSTMLVAQMVRPES"
     gene            complement(913..2127)
                     /locus_tag="PLQ29_08050"
     CDS             complement(913..2127)
                     /locus_tag="PLQ29_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86636.1"
                     /translation="MVGKRLNVGLIAATLVAMLASCSDAREVTALQREQLFTLGYGVL
                     EDQLNLFSLEGNAPPAKTALAMRDGIFFVSNGNAAKVMTLSSFGDLLAMVYNPERNPP
                     PLTLQAAETGAVAQGRVARAYPFGSPGEIAVDSKRTIFVEDRVPDSRRSYDDSSMASL
                     EYVVLRFSRDGEYLDYLGQEGVGGTPFPSIVGLYITAADDCVVVCLTGMGWTAFVFDR
                     FGILESTVSIRRNELPRPPEEAGGIASLDRIVAAPDGSGLIIKIDYYREIVDQETHTQ
                     AGIEFSSSWAWVMDGSNGAYVERYELQPFESLVKDEARPVPRAWGLVGAAADTLFLAA
                     TDDDGATYYGLFDRVSRTIRRYSLKIEPDELLYASFSLSPEGILSAVLGSRYEARFVW
                     WRFDRLIGGLAP"
     gene            2216..>3255
                     /gene="secA"
                     /locus_tag="PLQ29_08055"
     CDS             2216..>3255
                     /gene="secA"
                     /locus_tag="PLQ29_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697621.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPG86637.1"
                     /translation="MAFNPLTALFGSKRERDIKALLPRLHAVNEREAWAMALKPEEFP
                     AKTAELKARVASGEPLDSVLPEAFALAREAARRVLGERSFDTQVLGGIVLHDGKIVEM
                     KTGEGKTLSSVAAAYLNSLEGKGVHVVTVNDYLAERDSRWMGSIYSYLGVSVGAILSD
                     MDNDARRAAYAKDITYGTNNEFGFDYLRDNMAMEPSRRVQRGHHYCVVDEIDSILIDE
                     ARTPLIISGAAEDDTFKINEVSRLCHHLVEVKKDPATGEYPKEEEGENLEGDYKIDEK
                     SKKVSFTSDGLAKMEDALKKRGLIQGSLFDEQNFEYVHYFTQAVRARELFTKDTEYVV
                     QEGLVQIVDEFTG"
BASE COUNT          617 a         1101 c         1066 g          471 t
ORIGIN      
        1 cggactccgg ccggaccatc tgcgccacga gcatcgtcga gagcatcggc accagctcgt
       61 cgcgtgccag caacgtgacg ctaccggcgc cggccgcggc cgcggccctg gaacagagca
      121 cggccgcgcc ggtagagcca taggaaccgg cgaaagcaca gacggctccg cgatcgccct
      181 tgtgacagtc cggatcgagt cctggcaggg cgtcttcgag gtcgccgggc tcgaggagga
      241 ccatcgtcga tgccgcaccg gccgatcgat cgaacactcc accgattggc aggatgcggc
      301 ccgcgtaggg ccggaagccc gggtagtaca gctccgcctt gagcggctcg acgcacagcg
      361 ttatatcggc gcataccggt gcgctggggt cggctgcgac agacccgagc gcgccgacgc
      421 cggaaggcac gtccatcgat acgacaggcc cccgagccga cgcgatcagg gcggccgcct
      481 gaacgagggc cgctcgagcg ggccccttga agccgactcc cgatagcccg tcgagcacca
      541 cgccggctct cgcgacggct tcatgagccg cgtcgtcgcc gaggccgata acgcgagcgc
      601 ccgccagccc ggcctcggct agccgccgcg aggccagggg gccgaggcta tcgggtacga
      661 gcgcgaccag gtccttccag ccggaaaagg ccaggcgccg gagaacggcc agcgcgtctc
      721 cggcattgtt accgccgccg cagacggccg cgatggacgg cgaacccgag gccgccgctt
      781 tcctcaaggc tggatcggca tcgatcgcgg cggccatacc gacggcggcg gcctccatca
      841 ggagatcgtc gccaaggcca tccctcgccg acgcgtcgag cgccagcgcc gaggcgaagc
      901 cgacgagcgg cctcacggcg cgagcccacc tatcaggcgg tcgaagcgcc accagacgaa
      961 gcgagcctcg taacgggagc cgagcacggc ggagagtata ccctccgggg acagcgagaa
     1021 cgaagcgtac aagagttcgt cgggctcgat cttcagcgag tagcgccgta tcgtgcgcga
     1081 aacccggtcg aacagcccgt agtaggtggc cccgtcgtcg tcagtcgccg ccaggaacag
     1141 cgtgtcggcc gcggccccga ccaggcccca ggctctcggt accggccgcg cctcgtcctt
     1201 gaccagggat tcgaagggct ggagctcgta gcgctcgacg taggcgccgt tcgaaccgtc
     1261 catgacccag gcccatgacg acgagaactc gatgccagcc tgggtatggg tctcctggtc
     1321 gactatctcc ctatagtaat cgatcttgat gatcaggccg gagccgtctg gcgccgcgac
     1381 gatccgatcg agcgaggcga tgcctcccgc ctcttcaggc ggcctcggta gctcgttacg
     1441 gcgtatcgag accgtcgact ccaggatgcc gaagcggtcg aagacgaagg ccgtccagcc
     1501 catgccggtc aggcagacga cgacgcaatc gtccgccgcg gtgatgtaga ggccgacgat
     1561 gctcgggaac ggcgtacccc cgacgccctc ctggccgagg taatcgaggt actcgccgtc
     1621 gcgggaaaag cgcaacacga cgtactcgag gctggccatc gacgagtcgt cgtacgaccg
     1681 gcgggaatcg ggcacgcggt cctcgacaaa gatggttcgc ttcgagtcga ccgctatctc
     1741 gccgggcgag ccgaatggat aggcccgggc gacgcggccc tgggctaccg cgccggtctc
     1801 cgccgcctgc agggtcaggg gcggcgggtt acgctctgga ttatagacca tcgcgaggag
     1861 gtcgccgaac gacgacaggg tcatgacctt ggcggcgttg ccgttcgaga cgaagaagat
     1921 gccgtcgcgc atcgccaggg cggtcttcgc tggcggcgcg ttgccctcga ggctgaacag
     1981 gttgagctgg tcctcgagca cgccgtagcc gagggtgaag agctgttcgc gctgcaacgc
     2041 cgtaacctcg cgcgcgtcgg agcaggaagc gagcatagcc accagcgtgg ccgcgatcaa
     2101 acctacgttg agcctcttgc ctaccacgcc gcgacctccg gatcgagaat cggttccgcc
     2161 cgaccgcccg ggtcttgctt gaccgcttat cgttaaaaga tatagattaa acgacatggc
     2221 gttcaacccc ctgactgctc tcttcggctc gaagcgagaa cgagacataa aggcgctcct
     2281 gccccgcctc cacgcggtga atgagcgcga ggcctgggcg atggccctga agcccgagga
     2341 attccccgct aagacggccg agctcaaggc ccgcgtcgcc tccggcgagc cactcgactc
     2401 cgtattgccg gaggccttcg cactggccag ggaggcggcc aggcgtgtgc tcggcgagcg
     2461 ctccttcgac acgcaggtgc tcggcggcat cgtgctccac gacggcaaga tcgtcgagat
     2521 gaagaccggc gaaggcaaaa ccctgtcgtc ggtcgccgcc gcctacctga actcgctcga
     2581 gggcaagggc gtccacgtcg tcacggtcaa cgactacctg gccgagcgcg actcgcgctg
     2641 gatgggctcg atctactcct acctcggcgt ctcggtcggg gcgatcctct cggacatgga
     2701 caacgacgcg aggcgcgccg cctacgccaa ggacatcacc tacggcacca ataacgagtt
     2761 cggcttcgac tacctgcgcg acaacatggc catggagccg tcacggcggg ttcagcgcgg
     2821 ccaccactac tgcgtggtcg acgagatcga ctcgatcctg atcgacgagg cgcgcacgcc
     2881 gctgatcatc tcgggtgccg ccgaggatga caccttcaag atcaacgagg tttcgcgcct
     2941 ctgccaccac ctcgtcgagg tcaagaagga ccccgctacc ggcgaatatc ccaaggaaga
     3001 ggaaggcgag aacctcgagg gcgactacaa gatcgacgag aagagcaaga aggtatcctt
     3061 cacctccgat ggcctggcca agatggagga tgcgctcaag aagcgcggcc tcatccaggg
     3121 ctccctgttc gacgagcaga atttcgagta cgtccactac ttcacgcagg ccgtccgagc
     3181 ccgcgagctg ttcacgaagg acaccgagta cgtcgtccag gagggcctcg tccagatcgt
     3241 cgacgagttc accgg
//
LOCUS       DAOPHV010000282         3249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_40987_length_3249_cov_4.211960, whole genome shotgun sequence.
ACCESSION   DAOPHV010000282 DAOPHV010000000
VERSION     DAOPHV010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40987_length_3249_cov_4.211960"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PLQ29_08060"
     CDS             complement(<1..949)
                     /locus_tag="PLQ29_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPG86638.1"
                     /translation="MSQVETGAEDRTDEESARKALSEQQMVRREKLAKLQAEGRDPFA
                     MVTWKQTHHSEEIKRKFEELENKDVSIAGRVMSFRDIGKAAFIDLQDRDGRVQVYLNA
                     EGLGDEAYAHVKTWDLGDIVGVEGFVFKTRRGEISVHAKRMVLLTKAIEPLPEKFHGL
                     KDPEQRYRRRYLDMVMNREVMDTFRKRTQVIRAVREYLDDIGYIEVETPILSTIASGA
                     AARPFVTKSNALNLQLYLRIATELYLKRCIVGGMERVYEMGKDFRNEGIDIRHNPEFT
                     MIELYQAYADYNDMMELCENLCAYAATKVCGSTKVSYQGT"
     gene            complement(1089..2882)
                     /locus_tag="PLQ29_08065"
     CDS             complement(1089..2882)
                     /locus_tag="PLQ29_08065"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPG86639.1"
                     /translation="MEYTAYLITAPIAVLALVATYAALRRYGAEPPARSLGRYMLLAI
                     WLLVANILELVSPGEFATVLFAKLEYLALLLLPISFDWFCLRYTGHDRYIRPPARAVL
                     VAASLAMFAVVATNEWHGLFWSRLEFPVVEGFTTLRTSFGPFFWVAAAYTWLLTIVGA
                     FVVLRSYVFERGPYRVRSAAIAAGVLAPGLCNLVYILKLLPSLRKDFTPIGYALGALL
                     FFTGAYLNRSARVIPLARGVVLQELEEGVVFVDPAGRLADYNAFAESLLGLDGSMLGR
                     PAATIAELAPLATLLASASVSADGAAAASATIAERSIVARAKRIGDKREPFGQRGVAI
                     TLSDVTERARMQDELDAARGDLLKREHFAVIGRLSADIAHEIHDPLDYVSAQLAAVKS
                     SVAGAIGDEGVRAEVFKALRSVDEGLERIGNVSRSLLEYSKRGAGDEEPAPYDLSRGV
                     QNALELSRSDYARVATVDIDLVETPRVMARGSEIDRVLINILRNAAEAIREKASATGA
                     RGRIWVRTRIEGYTVVCEIGNDGTPLADRDGQRVFEEFFSSGSDGKGLGLSISRDVVE
                     KRHGGRLSLASRDPVVFRMELPMAHVSAPSP"
BASE COUNT          554 a         1039 c         1085 g          571 t
ORIGIN      
        1 cggtgccctg gtagctcacc ttcgtcgacc cgcagacctt ggtggccgcg taggcgcaga
       61 gattctcgca taactccatc atgtcgttgt aatcggcata ggcctggtac aactcgatca
      121 tggtgaactc cgggttgtgc cggatgtcga tgccctcgtt gcggaagtcc ttgcccatct
      181 cgtagacgcg ttccatgccg ccgacgatgc agcgcttgag gtacagctcg gtggcgatgc
      241 gcaggtacag ctgcaggttg agcgcgttgg acttggtgac gaagggcctg gccgcggcgc
      301 cgctcgcgat cgtggagagt atcggcgtct ccacctcgat gtagccgatg tcgtcgaggt
      361 actcgcgcac ggcgcggatg acctgggtgc gcttcctgaa ggtgtccatc acctcgcggt
      421 tcatcaccat gtccaggtag cggcgtcgat agcgctgctc gggatccttg aggccgtgga
      481 acttctcggg caggggctcg atcgccttgg tgagcaacac catgcgcttg gcgtgcacgg
      541 atatctcgcc gcggcgcgtc ttgaacacga agccttcgac gccgacgatg tcgccgaggt
      601 cccaggtctt cacgtgcgcg taagcctcgt cgcccaggcc ttcggcgttc aggtagacct
      661 ggacgcggcc gtcgcggtcc tgcaggtcga tgaaggcggc cttgccgatg tcgcggaagc
      721 tcatcacgcg gccggcgatc gatacgtcct tattctcgag ctcctcgaac ttccgtttta
      781 tctcctcgga gtggtgagtc tgtttccagg ttaccatggc gaagggatct cgaccctccg
      841 cctggagctt ggcgagtttc tcgcggcgaa ccatctgctg ttcgctgagc gccttgcgag
      901 cgctttcctc gtcggtcctg tcctctgccc cggtttctac ctggctcatg cgtactcctt
      961 cgcgagcgtc gtaagcgcgc tcggcggaag ggtattgtat gcggaagccg ctatccgttt
     1021 caagctcgcc ttagtgttac gaaccttatg ggctctacgg cgactcgtct cctacggcga
     1081 ctcgtctcct acggagacgg ggcggaaaca tgggccatcg gtagctccat cctgaagacg
     1141 actgggtcgc gggacgcgag gctcaggcgg ccgccgtgcc gtttctccac cacgtcgcgc
     1201 gagatggaca ggccgaggcc cttgccgtcg gaaccggagg agaagaattc ctcgaacacg
     1261 cgctgtccgt cgcggtcggc cagtggggtg ccgtcgttgc cgatctcgca gacgaccgtg
     1321 tagccctcga tccgcgtcct gacccagatc ctgcctcgcg cgccggtcgc cgaggccttt
     1381 tcgcgtatcg cctccgcggc gttgcgcagt atgttgatca gcacccggtc gatttcggag
     1441 ccgcgcgcca tcacccttgg cgtctcgacg aggtcgatgt cgacggtggc tacgcgcgcg
     1501 tagtcgctcc gcgacagctc gagcgcgttc tgcacccccc gggacaggtc gtacggcgca
     1561 ggctcctcgt cgcccgcgcc ccgcttggag tactcgagca ggctcctcga tacgttgccg
     1621 atgcgctcga gcccctcgtc gaccgagcgg agcgccttga acacctcggc gcgcacgccc
     1681 tcgtcgccga tcgccccggc gacgctcgac ttaacggcgg cgagctgagc cgacacgtag
     1741 tcgagcgggt catggatctc gtgggcgatg tcggccgaca atcgccctat gacggcgaag
     1801 tgttcgcgct tgagcaggtc tccccgggca gcgtccagct cgtcctgcat gcgagcgcgc
     1861 tcggtcacgt cggacagggt gatcgcgaca ccgcgctggc cgaagggttc gcgcttgtcg
     1921 ccgatgcgct tggcccgcgc gactatcgag cgctcggcaa tggtcgccga ggccgcggcg
     1981 gcgccgtcgg cagataccga ggcggacgcg agcaacgtgg ccagcggggc cagctcggct
     2041 atagtcgccg ccgggcggcc gagcatcgac ccgtccagcc ctagcagcga ttcggcgaac
     2101 gcgttgtagt ccgccagcct accggccgga tcgacgaaca cgacgccctc ctcgagctcc
     2161 tgcagcacca cgcccctggc cagcggtatg acccgggcgg aacggttcag gtaggcgcct
     2221 gtgaagaaca ggagggcgcc gagcgcgtag ccgatcggcg tgaagtcctt cctgaggctg
     2281 ggtaatagct taaggatata gacgaggtta cagaggcccg gggccaggac gccggcggcg
     2341 atggcagccg agcggaccct gtacgggccg cgctcgaata cgtacgagcg taacaccacg
     2401 aacgcgccga cgatagtcag cagccaggta tacgcagcgg caacccagaa gaacggcccg
     2461 aaactcgtgc ggagcgtggt aaagccctcg accaccggga actcgagccg gctccagaat
     2521 aggccgtgcc actcgttcgt cgccacgacg gcgaacatgg ccagcgacgc cgcgacgagt
     2581 acggcccggg ccggcggccg gatgtagcga tcatggcccg tgtaacgcag gcagaaccaa
     2641 tcgaaggaga tgggcaggag caggagggcg aggtactcga gcttcgcgaa cagtaccgtc
     2701 gcgaattcgc cgggagacac tagttcgagg atattggcga cgaggagcca gatcgccagc
     2761 agcatgtagc ggccgagcga cctggccggc ggttcggcgc cataccgcct gagcgccgcg
     2821 taggtggcga cgagggccaa tacggctatc ggggccgtta tcaggtatgc cgtgtattcc
     2881 atagtggtga aagtatacta cgagacgacg ccgagctgga tataaaaagg ccgccgtcac
     2941 ctgcggcaac ggcggccaaa cgagataagg aggactctgc cactaatggc tcgggagcta
     3001 gccgaggtta ccgaaaatcg ttcaagcccg cgccgggggg ctggcggccg cctcgagcca
     3061 gaggttcagc atccgcaggg ccctgtccgg ccgcttgaag gcggccggca tcgtaaggaa
     3121 tcctattgaa cgtcgccgct gaaagcggcg tgcgatacgg cggcccggcg catgcgcgcc
     3181 gggaccgtat cgagacgttc aggcccgcgt ctgggggctg gcagccgcct cgagccagag
     3241 gttcagcat
//
LOCUS       DAOPHV010000283         3241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_41119_length_3241_cov_3.790019, whole genome shotgun sequence.
ACCESSION   DAOPHV010000283 DAOPHV010000000
VERSION     DAOPHV010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41119_length_3241_cov_3.790019"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..2083
                     /locus_tag="PLQ29_08070"
     CDS             5..2083
                     /locus_tag="PLQ29_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HPG86640.1"
                     /translation="MQYDERLDDYLGNFTDDEAQRIRSARAWAAELHSGSTRASGEPT
                     VTHPERVAAILAGMGMDADSVVAGLLHQAIEDSLATGAQVGERYGERVGALVDELSRM
                     ATLKAKNKTIQAAETIRKMLFAMTRDLRVIIVKLADKLDNMRTLKWLPEADRRRIAAE
                     CVDVFAPLADRLGISWMKDELEDLALKEINREAFDQIKLLVAAKKPEREAFLERARAE
                     IDAAARADGLNVEVMSRAKHFYSIYQKMRRRAKGADELYDLSGIRVLCDEESQCYAVL
                     GIVHGLWKPLDGRFKDYIAMPKSNGYRSLHTTVMSYDGTLLEIQIRTREMHRVAEFGV
                     ASHWLYKKGTSAEIVRPEDLSIVNRLKDWSAMLEEGSDFLDDIKREILKDSIIVFTPK
                     GDAIELPAGSTPLDFAYAIHSDVGNRCLAAKADGAIVPLDSELRNTQVVDIVTGAAAR
                     PNVNWLKIVRTSKARSKIRSWLVAEGTVLAIDKNVVARKPGERHDERGKAGERRPELE
                     VRHGEAKHGEAKHGEPRQPEQAAGAMDYRPYVAGEATDSSKAGISVSGAENLLIRFAS
                     CCKPAVGDRIVGYVSRGRGIIVHRASCKNLAAIPEFEQRRVEVGWESAPSLTRRYHVT
                     ARRVLDLFSEIENAVRKHGGRLVEGRLEDGAEALSGFFTMAYPSAEDARTAERNLKHV
                     PSILKIKRVD"
     gene            2247..>3241
                     /locus_tag="PLQ29_08075"
     CDS             2247..>3241
                     /locus_tag="PLQ29_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86641.1"
                     /translation="MNNGFVRWFLVKHAGAEPMERQQIGALLYTLAAIGVGAIAVAVV
                     MANPVIRLALIVLVLLSAALMLLVRAGFAVFSSVAATTLLSSMFAAIPFLQPYRHEYE
                     LYLLATLECFALIITGLIARRRWQSLVVLASALAALVSDFALRVIPNAGEGGARYSDI
                     VITVLVVVIATAIERAIKSRDSVLLGMAKAESAKNLAQIEKLEGVIRSSGDALGLGVA
                     ARDSAEATERLVDEMKATLEAAGKEFQDLERSASVIAESYLRIGESSRLVESKVSDQS
                     AVISESSAAIEEMTASVNNIAAIADARLAAIATLKATTDEGTGQMASSADALRDM"
BASE COUNT          600 a         1056 c         1070 g          515 t
ORIGIN      
        1 aggcatgcag tacgatgaac ggctcgacga ctacctaggt aattttacgg acgacgaggc
       61 gcaacgcata cggtccgcgc gcgcctgggc ggccgagttg cattcgggca gcacgcgggc
      121 gtcgggcgaa ccgaccgtga ctcacccgga acgggtcgcc gccatcctgg ccggcatggg
      181 catggacgcc gacagcgtcg tcgccggact cctccatcag gccatcgagg actccctggc
      241 cacaggcgcg caggtaggag agcgttacgg cgagcgggtc ggcgctctcg tcgacgagct
      301 gtcgcgcatg gctaccctca aggccaagaa caagaccatc caggcggccg agaccatacg
      361 caagatgctg ttcgcgatga cccgcgacct gcgcgtcatc atcgtcaagc tggccgacaa
      421 gctcgataac atgcgtaccc tcaaatggct gcccgaggcc gaccggcgcc gcatcgctgc
      481 cgagtgcgtc gacgtcttcg cgccgctggc cgaccggttg ggtatcagct ggatgaagga
      541 cgagctcgag gacctcgcgc tcaaggagat caaccgcgag gccttcgacc agatcaagct
      601 gctcgtcgcc gccaagaagc ccgagcgcga agccttcctg gaacgggcgc gcgccgagat
      661 agacgcggcg gctcgcgcgg atggactaaa cgtcgaggtg atgtccaggg ccaaacactt
      721 ctattcgata taccagaaga tgcgcaggcg cgccaaaggc gcggacgagc tgtacgacct
      781 gtcgggcatc cgcgtgctat gcgacgagga gagccaatgc tacgccgtcc tcggcatcgt
      841 gcatgggctc tggaagcctc tcgatgggcg cttcaaggac tacatagcga tgcccaagtc
      901 caatggttac cgtagcctgc acaccacggt gatgtcctac gacggtaccc tgctcgagat
      961 ccagatacgt acccgcgaga tgcaccgagt cgccgagttc ggtgtcgcgt cgcactggct
     1021 gtataagaag ggcacgagcg ccgagatcgt ccgacccgag gacctgtcca tcgtcaaccg
     1081 gctcaaggac tggtccgcca tgctcgagga gggctcggac ttcctcgacg acatcaagcg
     1141 ggagatcctc aaggattcga tcatcgtgtt cacacccaaa ggcgacgcga tcgagttgcc
     1201 ggcgggctcg acgccgctcg acttcgccta cgcgatccac tcggacgtcg gcaaccgttg
     1261 cctggccgcc aaggcggatg gcgccatcgt cccgctcgac tctgaactca gaaacactca
     1321 ggtcgtcgac atcgtcaccg gggccgccgc caggccgaac gtcaactggc tcaagatagt
     1381 caggacaagc aaagcgcgct ccaagatccg ctcctggctc gtcgccgaag gcaccgtgct
     1441 cgctatcgat aagaatgtcg tcgcgcgcaa gcccggcgag cggcacgacg agcgcggcaa
     1501 ggccggcgag cgcaggccgg aacttgaggt gcgccatggc gaagccaagc atggcgaagc
     1561 caagcatggc gagccgaggc agcccgagca ggcggccgga gcgatggact ataggcccta
     1621 tgtcgccggc gaggccaccg actcgtcaaa ggccggcatc agcgtctcgg gcgccgagaa
     1681 cctcctcata cgcttcgcct cgtgctgtaa gccggccgtc ggggatcgca tcgtgggcta
     1741 cgtctcgcgc gggcgcggca tcatcgtgca ccgagcctcg tgcaagaacc tggccgccat
     1801 ccccgagttc gagcagcgcc gcgtcgaggt gggctgggag tcggcgccgt cgctgacgag
     1861 gcgctaccac gtcacggccc gccgcgtgct cgacctgttc tcggagatag agaacgcggt
     1921 gcgcaagcac ggcgggcgcc tcgtcgaggg ccgcctcgag gacggcgcag aggcgctgtc
     1981 gggattcttc acgatggcgt atcccagcgc cgaggacgcg cggacggcgg agcgcaacct
     2041 gaagcacgtt ccgtcgatcc ttaagatcaa gcgcgtcgac tagccgcccg gccccggatc
     2101 gggcgcggat gtattgatat taccagacgc ttgaatttgc tgatttgaat atatatactc
     2161 cgcgctatta tgcttacagg gcgcggccgg accgcgcatg cggttatgtc acctccgcgg
     2221 tcagcggcga tggggaaggg aattacatga acaacggatt cgtcagatgg ttcctggtca
     2281 agcacgccgg cgcggagccg atggagcggc aacagatcgg ggcgctgctg tatacgctgg
     2341 cggcgatcgg ggtgggagct atcgccgtcg ccgtagtcat ggccaacccg gtcatccggc
     2401 tggccttgat cgtcctcgta ctgctgtccg ccgcgctcat gctcctggtg cgcgcgggct
     2461 tcgccgtctt ctcgagcgta gcggccacga cgctcctctc gtcgatgttc gccgccatcc
     2521 cgttcctgca gccctacagg cacgagtacg agctgtacct gctggcgacg ctggaatgct
     2581 tcgcgctcat catcaccggc ctgatcgcca ggcggcgctg gcaatcgctg gtggtcctgg
     2641 ccagcgccct cgccgcgctt gtatcggact tcgcgctccg cgtgatcccg aacgcgggcg
     2701 aaggcggcgc cagatatagc gacattgtca tcaccgtcct cgtggttgtc atcgctaccg
     2761 cgatcgagcg cgccatcaag tcgcgcgact ccgtcctgct cggcatggcc aaggccgagt
     2821 cggccaagaa cctggcccag atagagaagc tcgagggcgt gattcggtcg agcggcgacg
     2881 cgctcggcct cggcgtcgcg gcccgggact cggcggaggc gacggagcgg ctcgtcgacg
     2941 agatgaaggc gacgctcgag gcggccggca aggagttcca ggacctggag cgcagcgcct
     3001 cggtcatcgc ggagtcgtac ctgcggatag gcgagtcgtc gcgcctggta gagtcgaagg
     3061 tttccgacca gtccgccgtc atctcggagt cgtcagccgc gatcgaggag atgaccgcgt
     3121 cggtgaacaa catcgcggcc atcgccgacg cgcgcttggc ggcaatcgcc acgctcaagg
     3181 ccacgaccga cgagggcacc gggcagatgg ccagctcggc cgacgccctg cgcgacatgg
     3241 a
//
LOCUS       DAOPHV010000284         3221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_41487_length_3221_cov_4.689514, whole genome shotgun sequence.
ACCESSION   DAOPHV010000284 DAOPHV010000000
VERSION     DAOPHV010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41487_length_3221_cov_4.689514"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2375)
                     /locus_tag="PLQ29_08080"
     CDS             complement(<1..2375)
                     /locus_tag="PLQ29_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86642.1"
                     /translation="MMFDLGNLVAIGITLIILLAYRILDRDNRSLEKVKKYAEKLREE
                     LSAHADQRAEDLKAYAIDLEVHQKAAKEVLRRVQLAEEALSAKADQIGGMAARIAEYD
                     KALAELKDMSSRVDENLSVIHDESAFVDGVARTLKAAKLEIEALKSSIPSIREGIAAD
                     AKDMMDDLRQAYSDELRSAMDDAQAQVEALRERAEQGTAEVAETNAEAVRAAEQRYRA
                     IDAMLADAFKRAREEGERLEDESFKRLEAQIDARGARLGEAIDTRFNALRDQAKEKVS
                     ETQGLLKSFKAEWRKDADELLAGARAEAEATVDKVGARMEDAEARAAKAEAKALETAQ
                     AVHAKVKDQLKAYQEDLAERQAAIRGAIKEGLVDTKAAAEGAVREMAESLSSFKSRID
                     ETAAEQAARVAAMDASLGAAEKRAADAVAALSAKFTDRGADLEKRVLAGFEARAAELR
                     GVVEQGLTRLEGIRLDADRMESALRESMAGVERRVEEDFALFGKDLAARQASFEADFK
                     DDSARVKSAVKDLESDLNALKSKAYADVSEKLKIFEDEFFADLRQRSDDANERFAVWR
                     AEMDERLEASIREADAARAETDRAWSEEARARLAETQGRVQEQLDKLGAQVDAHRSAI
                     SERVGEADEALSSLKASVKADLDDARAAANAFMAAELDRWKHDASERVRGAERQAEAD
                     GKALEEVTERARAAFTEARATVLAHAEAWKKEYAESMRAAETEHSAAIAALGDSFKAD
                     VAAISDNWEKERRKVIEAAKLERDALSKDVRALSDEVGRFRQELAQKTSQAL"
     gene            2571..>3221
                     /locus_tag="PLQ29_08085"
     CDS             2571..>3221
                     /locus_tag="PLQ29_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86643.1"
                     /translation="MKKMLAATLIALAMMASSCAVVDSLFYNADGVKASELEAFEGSA
                     ASNQAEAKAALIAGIDSGALPVAAFAVSGPAGDVIARAFPRLLPAYSEPEAGSTASRA
                     APTATVNSDGSVVMSWTGDGELVSSNPTVTLSGSLAYTVSADKNEELTSIAMDASLKA
                     DLEAEIGAYAAAGLKGAMMNLKAKGDASLTGSVSGFTAKGYAALSLNAGMSLDEAAG"
BASE COUNT          422 a         1112 c         1076 g          611 t
ORIGIN      
        1 agggcctggg acgtcttctg ggcgagctcc tgtcggaagc ggccgacctc gtcggacagc
       61 gcgcgcacat ccttcgagag ggcgtcgcgc tcgagtttgg ccgcctcgat aaccttgcgg
      121 cgctccttct cccagttgtc ggagatggcc gcgacgtccg ccttgaacga gtcgccgagc
      181 gcggctatgg cggccgagtg ctcggtctcc gcggcccgca tcgattcggc gtattccttc
      241 ttccaggctt cggcgtgggc gagcacggta gcccgcgcct cggtgaacgc ggcgcgggcc
      301 cgctccgtta cctcttcgag cgccttgccg tcggcctcgg cctggcgctc ggcgccgcgg
      361 acccgctcgc tcgcgtcatg cttccagcgg tcgagctccg cggccatgaa tgcgttggcg
      421 gcggcgcgcg cgtcgtcgag gtccgccttg acggacgcct tgagggacga aagggcctcg
      481 tccgcctcgc cgacccgctc gctgatggcg gagcggtggg cgtcgacctg ggctccgagc
      541 ttgtccagct gctcctggac gcggccctgc gtctcggcca gcctggcgcg cgcctcctcg
      601 gaccaggcgc ggtcggtctc ggcgcgggcc gcgtcggcct cgcggatgga ggcctcgaga
      661 cgctcgtcca tctcggcgcg ccagacggcg aagcgctcat tggcgtcgtc gctacgctgg
      721 cgcaggtcgg cgaagaactc gtcctcgaat atcttgagct tctcggacac gtcggcgtag
      781 gctttgctct tgagcgcgtt caggtcgctc tcgaggtcct tgaccgccga cttgaccctg
      841 gccgagtcgt ccttgaagtc ggcctcgaac gaggcttgcc tggcggccaa atccttgccg
      901 aatagcgcga agtcctcctc cacgcggcgc tcgacgccgg ccatggactc gcgcagggcc
      961 gactccatgc ggtccgcgtc cagcctgatg ccctcgagcc tcgtgagccc ctgctccacg
     1021 acgccgcgca actcggcagc gcgggcctcg aaaccggcga gcacgcgctt ctcgaggtcg
     1081 gcgccgcggt cggtgaactt ggcgctcagg gccgcgaccg cgtcggcggc ccgcttctcg
     1141 gcggcgccga gcgacgcgtc catcgcggcg acgcgggcgg cctgctccgc ggccgtctcg
     1201 tcgatcctgg acttgaacga cgacagggac tcggccattt ccctgacggc gccctcggcc
     1261 gccgccttgg tgtctactaa gccttccttg atcgcgccgc ggatcgcggc ctgtcgctcg
     1321 gcgaggtctt cctggtaggc cttgagctgg tccttgacct tggcgtgtac cgcctgggcc
     1381 gtctccaggg ccttggcctc ggccttggcc gcgcgggcct cggcgtcttc catgcgggcg
     1441 ccgaccttgt cgacggtcgc ctcggcctcg gcgcgggcgc cggccaggag ttcgtcggcg
     1501 tccttgcgcc attccgcctt gaacgacttg aggaggccct gggtctcgga gaccttctcc
     1561 ttggcctggt cgcgcagagc gttgaagcgg gtgtcgatgg cctcgccgag cctggctccg
     1621 cgggcgtcaa tctgggcctc gagccgcttg aagctctcgt cctcgagccg ctcgccttcc
     1681 tcccgcgcgc gcttgaacgc atcggccagc atcgcgtcga tggcgcggta ccgctgctcg
     1741 gccgccctga ccgcctcggc gttggtctcg gccacctcgg cggtaccttg ctcggcgcgt
     1801 tcccggagcg cctcgacctg ggcctgggcg tcgtccatcg ccgaacgcaa ctcgtcggag
     1861 taggcctggc gtaggtcgtc catcatgtcc ttggcgtcgg ccgcgatacc ttcgcgtata
     1921 ctgggtatcg acgacttcag ggcctcgatc tcgagcttgg cggccttgag ggtacgggcg
     1981 acgccgtcga cgaaggcgct ctcgtcgtgg atgaccgaca ggttctcgtc gaccctggag
     2041 ctcatgtcct tgagctcggc gagcgccttg tcgtactcgg ctatgcgcgc ggccatgccg
     2101 cctatctggt cggccttggc gctcagcgcc tcctcggcca gctgcacccg gcgcagcact
     2161 tccttggcgg ccttctggtg cacctcgagg tctatcgcgt aggccttgag gtcctcggcg
     2221 cgctggtccg cgtgggccga cagctcctcg cgcagcttct cggcgtattt cttgaccttc
     2281 tcgagcgagc ggttatcgcg gtcgagaatt cggtaggcga gcaggatgat cagcgtaatg
     2341 cctatggcca cgaggttgcc aaggtcgaac atcattattt ctcccattat cgattccgcc
     2401 tgggcgtgcc ggcggccggg atcggcccga ttggagtcag tatatagcac tatggaggaa
     2461 taataaagaa tcgagcttcg tcgacaggcg tgtcttgtaa aatctccatt ctatgctatt
     2521 atcatggtat ctatattgaa acgttgaaca tgtccgacaa tggaggtcgt atgaagaaga
     2581 tgctggcggc gaccctgatc gccctagcca tgatggcgtc gtcgtgcgcc gtcgtcgatt
     2641 cgctgttcta taacgccgac ggcgtgaagg cgtcggagct cgaggccttc gaggggagcg
     2701 ccgcgtcgaa ccaggccgag gccaaggcgg ccctgatcgc cggcatcgat tccggcgccc
     2761 tgcccgtcgc cgcgttcgcc gtatccggcc ccgccggcga cgtgatagcc cgcgcgttcc
     2821 cgaggttgct acccgcgtac tcggagcccg aggccggttc cacggcgtct cgcgccgctc
     2881 cgaccgccac cgtcaactcc gacggtagcg tcgtgatgag ctggacgggc gacggcgagc
     2941 tggtctcgtc gaacccgacg gtcacgctga gcggcagcct cgcctatacc gtatcggccg
     3001 ataagaacga agagctgacg tccatcgcca tggacgcgtc cctgaaggcc gacctcgagg
     3061 ccgagatagg cgcgtacgcg gcggccgggc tcaagggcgc catgatgaac ctgaaggcca
     3121 agggcgacgc gtcgctcacc ggttcggtaa gcggcttcac ggccaagggc tacgccgccc
     3181 tgtcgctcaa cgcgggcatg tccctcgacg aggcggccgg c
//
LOCUS       DAOPHV010000285         3220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_41497_length_3220_cov_4.480885, whole genome shotgun sequence.
ACCESSION   DAOPHV010000285 DAOPHV010000000
VERSION     DAOPHV010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41497_length_3220_cov_4.480885"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="PLQ29_08090"
     CDS             <1..991
                     /locus_tag="PLQ29_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPG86644.1"
                     /translation="AWTMGGTTMDTAAVGVEGRVLEIQRMSTEDGPGLRTTLFLKGCS
                     LRCRWCHNPESIDPRPQPRWTAVRCLGCGACVAACPPGALSLGPEGVAIDRSRCDGCG
                     ACAAECPATAIELWGVSRDSASLARELLKDEAYFGEDGGVTVSGGEACLQPRFVADLL
                     SRLKASGIGTALDTCGACSSRQFDEAAEYADLILYDLKDSEPERHRRNVGGELERVHD
                     NFRRALGLVRAGGKRLWVRTPIIPGLTDSDDVVRGIAAFLAREAGGLVERWELCAFNN
                     LCSGKYRSLGRAWELEGAPLKRAEEMGALVGLAVAEGWPADRVRWTGMTRRES"
     gene            1022..1897
                     /locus_tag="PLQ29_08095"
     CDS             1022..1897
                     /locus_tag="PLQ29_08095"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPG86645.1"
                     /translation="MVTKDCFDEGDLRHFEPDGKVGLLATVDPDGLPHVTLITAMQAT
                     DTRRLIFGQFCEGRGKRYAEERRRVGFLFLSLDKRYWRGTADWTGKARSGPEHELMNK
                     KPMWRYNSYFGIHTVHYLDLVSTTAAASVDMGAVVAGAAKAALAGRRASLALGLEPHP
                     GAMNPWTRGLISRLGNLKFVSWIGDDGYPRIAPQLAATCLGGGRVYLPGAEHGDELAE
                     IRDGASVALFAMALSMEDVLVRGRFRRTGGRPGGLPAGRASGCLDVDWVYNSMPPVAE
                     RIYPPVRLAEKVTAF"
     gene            complement(1894..2631)
                     /locus_tag="PLQ29_08100"
     CDS             complement(1894..2631)
                     /locus_tag="PLQ29_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPG86646.1"
                     /translation="MLKSILVLSYFWLSAAIASPFALIYILASALGLGRALRPALGVV
                     TRAWSKSVLAAVGARTIVEGIENVPDDEKLCFVANHQGDLDIVMMLAYMPRPVGFVAK
                     KEAAWFPFINLWIAALGSAFIDRGSPRQGKRAIDRGVRSVERGNAMVIYPEGTRSRGP
                     SMLPFKKGAFKLATRPGATIVPVTIDGAYKVWELHGRIESADVRIVVHPPIRTAGLDA
                     EARKELPSLVEATIASALPASSAPGPV"
     gene            2746..>3220
                     /locus_tag="PLQ29_08105"
     CDS             2746..>3220
                     /locus_tag="PLQ29_08105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPG86647.1"
                     /translation="MIGEASSKGGDPFIEYGRKHPGASASFNAPGRGGEPASMRISGL
                     IDSDTAEDFLEASLKALGTLSRGESINLDLKDVRFVSSTGVGTLTKLLAAAESLGVSL
                     RICAVSPACEDVFSILGLLDYFGIEPSAGSGAAQGGGRYEGRDGEAGASPSGGLVL"
BASE COUNT          472 a         1066 c         1178 g          504 t
ORIGIN      
        1 ggcatggacg atggggggaa cgacgatgga tacggcggcg gtgggcgtcg aggggcgggt
       61 cctcgagatc cagcggatga gcaccgagga cggaccgggc ctcaggacga cgctattcct
      121 caagggctgc tcgctacgct gccgctggtg ccataacccg gagagcatcg acccgcggcc
      181 ccagcctcgc tggacggcgg tgcgctgcct gggctgtggc gcctgcgtcg cggcctgccc
      241 gccgggagcg ctgtcgctcg ggccggaggg cgtcgccatc gaccggtcgc gttgcgacgg
      301 ctgcggcgcc tgcgcggccg agtgcccggc gaccgcgatc gagctgtggg gcgttagccg
      361 cgactcggcc tcgctcgcga gggagctcct gaaggacgag gcgtacttcg gcgaagacgg
      421 cggcgtgacc gtctcgggcg gcgaggcctg cctgcagccg cgcttcgtgg ccgacctgct
      481 gtcgcggctc aaggcctccg gcatcggcac ggcgctcgac acctgcggcg cctgctcctc
      541 gaggcagttc gacgaggcgg ccgagtacgc cgacctcatc ctgtacgacc ttaaggattc
      601 cgagccggag cggcaccggc gcaacgtcgg cggcgagctc gagcgggttc acgacaactt
      661 caggcgcgcc ctcggcctgg tccgagccgg aggcaagcgg ctctgggtgc gcaccccgat
      721 catcccgggg ctgaccgact cggacgacgt cgtgcgcggc atcgccgcct tcctggcgcg
      781 ggaggccggc ggcctcgtcg agcgctggga gctgtgcgcc ttcaacaacc tgtgctcggg
      841 caagtaccgg tcgctcggcc gggcctggga gctcgagggc gcgccgctca agcgggctga
      901 ggagatgggg gccctggtag gcctggccgt ggccgaaggc tggccggccg accgggtgcg
      961 ctggaccggc atgacgaggc gcgaatcgta gggaccggct accggccggg gggaggcatc
     1021 gatggttacc aaggattgtt tcgacgaggg cgacctgcgg catttcgagc cggacggcaa
     1081 ggtcggcctc ctggcgaccg tcgacccgga cggcctgccg cacgtgacgc tgatcaccgc
     1141 gatgcaggcg accgatacgc ggcgcctgat cttcggccag ttctgcgagg gccgcggcaa
     1201 gcgctacgcc gaggagcggc gcagggtcgg cttccttttc ctgtcgctcg acaagcgcta
     1261 ctggcgcggt acggccgact ggacgggcaa ggcccgctcg gggccggagc acgagctgat
     1321 gaacaagaag ccgatgtggc gctacaactc gtacttcggc atccataccg tgcactacct
     1381 ggacctggtc tcgacgacgg ccgcggcctc ggtggacatg ggcgccgtcg tcgccggagc
     1441 ggccaaggcc gccctggccg gtcggcgggc atccttggcg ctcgggctcg agccgcatcc
     1501 cggagcgatg aatccctgga ccagggggct tatatcgagg ctcggcaacc tcaagttcgt
     1561 ctcgtggata ggcgacgacg gctacccgcg catcgcgccc cagctggcgg cgacctgcct
     1621 ggggggcggc cgcgtctacc tgccgggcgc ggagcacggc gacgagctgg cggagatacg
     1681 ggacggggcg agcgtggcgc tgttcgcgat ggcgctcagc atggaggacg tcctcgtgcg
     1741 cggccgcttc cgcaggacag gcgggcggcc gggaggactc ccggcaggcc gagcctccgg
     1801 ctgcctcgac gtcgactggg tctataactc gatgccgccg gtcgccgagc gtatctaccc
     1861 gccggtcagg ctcgccgaga aggtgaccgc cttctagacc gggccggggg ccgagctggc
     1921 gggcagcgcc gaggctatcg tcgcctcgac cagagacggt agctctttcc tggcctcggc
     1981 gtccaggccg gcggtgcgga tgggcgggtg gacgacgatg cgcacgtccg ccgactcgat
     2041 gcggccatgg agttcccaga ctttgtaggc gccgtctatc gtcaccggta cgatcgtcgc
     2101 ccccggtctc gtagccagct tgaaggcgcc cttcttgaac gggagcatcg acgggccgcg
     2161 actcctcgtg ccctcggggt agatcaccat cgcgttgcct cgctcgacgc tacggacgcc
     2221 gcggtcgatg gcgcgcttgc cctgccttgg gctaccccgg tcgatgaagg ccgagccgag
     2281 ggccgctatc cataggttga tgaaggggaa ccaagccgcc tctttctttg cgacgaagcc
     2341 gaccggcctg ggcatgtacg cgagcatcat cacgatgtcg agatcgccct ggtggttggc
     2401 gacgaagcat agcttctcgt cgtcgggcac gttctcgatg ccctcgacga tggtgcgggc
     2461 gcctacggcc gccaggacgc tcttggacca ggctcgcgtc accacgccga gggccggcct
     2521 gagcgcccgc ccgagcccga gcgcgctcgc caggatgtag atcagcgcga atggcgaagc
     2581 tatcgcggcg ctgagccaga agtatgacag tacgagtatc gacttgagca tcgggaccaa
     2641 cctccgggat acgaaacggt tcgagggccg ctccatcatc gcgaaattcg actccgatgc
     2701 caagccgggg ttgcggccgc tgttcaagcc actatactta tcctcatgat cggcgaggct
     2761 tcctctaagg gcggagatcc attcatcgag tacgggcgca agcatcccgg cgcctcggcg
     2821 agctttaacg ctcccgggcg cggcggtgag ccggcgtcga tgcgcatctc gggcctgata
     2881 gacagcgata ccgccgagga tttcctcgag gcgtcgctga aggcgctcgg gaccctgtca
     2941 cggggcgagt cgatcaacct cgacctcaag gacgtgcgct tcgtgtcgtc cacgggggtg
     3001 gggacgctta ccaagttgct cgcggcggcc gagtcgctcg gcgtgtcgct acgcatctgc
     3061 gccgtatcgc ccgcctgcga ggacgtgttc tccatcctcg gcctgctcga ttacttcggc
     3121 atagagccgt cggccgggtc cggggcggct cagggcggcg gccgctacga ggggcgcgac
     3181 ggcgaggccg gggcctcgcc gtcggggggg cttgtcctag
//
LOCUS       DAOPHV010000286         3215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_41600_length_3215_cov_4.325633, whole genome shotgun sequence.
ACCESSION   DAOPHV010000286 DAOPHV010000000
VERSION     DAOPHV010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41600_length_3215_cov_4.325633"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="PLQ29_08110"
     CDS             <1..1119
                     /locus_tag="PLQ29_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPG86648.1"
                     /translation="YFNRAVVNLWRLCPEARDRRAAHSGARPAGDTGVAGLEFDLLDH
                     MRRALDAAKATGPGYVRPIRIRLDYTLTVRPGAVPEGETLRCWLPLPRVGGQHLDLAI
                     LATDPADATAAPEEAPQRTLYLERPASPAGSPTVFSASYEFTSRDYAPLVDPERVRPT
                     DASTELYREYTAERPPHLVLSPELRGLASSIVGSEANPYRKARLIFAWFDRNVTYTAT
                     PEYSTIPNLSEYCLRERRGDCGVQAMLFIALCRAVGVPARWQSGLTLLPGKMNLHDWA
                     LFHVEPYGWLPADPSRGFRDTDDEELRWFYLGGIDAFRAVVNDDYGRSLHPPKRFARS
                     EPADFQRGEVEWSGGNLYFGDWDYEFGIEYRELDSGGA"
     gene            1197..1847
                     /locus_tag="PLQ29_08115"
     CDS             1197..1847
                     /locus_tag="PLQ29_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86649.1"
                     /translation="MKVTKSLVFIAWFLLPAIPAALYAASAGGFPDAYSVSVALGAYA
                     YALLAAQPALAARPAFIVKAIGAKGSATLHGVAPVLALGLAAAHRALKLGLGFEAGTT
                     QAVIGAASFWFLVAASVAAVILLAAFGGALGKALKPLRAWVAERLGLNYKAARAAHGL
                     AALAVVALFVHLSLASSSDLSFNPAGPAWLAAWTLVSFGVYARSRLSARSAGKASR"
     gene            1844..2380
                     /locus_tag="PLQ29_08120"
     CDS             1844..2380
                     /locus_tag="PLQ29_08120"
                     /inference="COORDINATES: protein motif:HMM:NF015318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON domain-containing protein"
                     /protein_id="HPG86650.1"
                     /translation="MKRVVFIMVALMAAASAASAQDLVRSSAAPSVDGIAAAGEYSTW
                     FERSEIRVGLSLSADGSTLYVAVDAPTAGWVAVGLGSLKMDGAYMVLAYVEDSGTVFI
                     GEETGYGRSHRPNAEKRLRASAVREANGRTTLELALPSAGLADGASLKALFAYGRRDD
                     RITKHVRYITAELPIVRR"
     gene            complement(2392..>3215)
                     /locus_tag="PLQ29_08125"
     CDS             complement(2392..>3215)
                     /locus_tag="PLQ29_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPG86651.1"
                     /translation="LIYGKFGFPALGVAGAAIATVLGNCAACAMAVYALFRRRSGERS
                     FASFGLTRENLVPNPAMLKRIVRIGLPSAAEQLALRAGLVIYTITITALGTEVFAAHQ
                     IVLSILNISFVNGQAFGIAATSLAGQALGRGSPDAARRASGACQKLGAVVSTVMGVLM
                     FVFRRSLVAMFTAEPAIIAISSGVMLLVAFIQPFQSSFQIYAGALRGAGDSLYPAISL
                     AAGILIIRPSLSYAFIHLLGLGLFGAWLALLADQVVRFGLIAIRFRGGKWVHTRV"
BASE COUNT          490 a         1130 c         1106 g          489 t
ORIGIN      
        1 tacttcaacc gggccgtcgt caacctgtgg cggctctgcc ccgaggcgcg cgaccgccgg
       61 gcggcccact ccggcgccag gcctgcgggg gatacgggag tagccggcct cgagttcgac
      121 ctgctcgatc atatgagacg agcgctcgac gctgccaagg ccacgggtcc gggctacgtg
      181 aggccgatca ggatcaggct cgactatacg ctgaccgtgc gcccgggcgc cgtacccgag
      241 ggcgagacgc tacgttgctg gctaccgttg ccgcgcgtcg gagggcagca cctcgacctg
      301 gccatactcg ccaccgatcc cgccgacgcg acggcggccc ccgaggaagc gccgcaacgg
      361 acgctgtacc tggaacggcc cgcgtcaccg gccggctcgc cgacggtttt ctcggcgagc
      421 tacgagttca cgagccgcga ctacgccccg ctcgtcgatc cggagcgggt acggcccacg
      481 gatgcatcga cggagctgta tcgcgagtat acggccgagc gcccgcccca cctggtcctg
      541 agcccggagc tccgcggcct cgcctcgtcc atcgtcggct ccgaggcgaa cccctaccgc
      601 aaggccaggc tgatcttcgc gtggttcgac cgcaacgtga cctataccgc cacgccggag
      661 tattcgacta tcccgaacct ctccgagtat tgcctacgcg aacggcgcgg cgactgcggc
      721 gtccaggcca tgctgttcat cgcgctgtgc cgcgcggtcg gcgtgccggc gcgctggcag
      781 tccggcctga ccctgctacc cggcaagatg aacctgcacg actgggcgct gttccacgtc
      841 gagccctacg gctggctacc ggccgacccg tcgcgcggct tcagggacac ggacgacgag
      901 gagctacgct ggttctacct cggcggcatc gacgcgttca gggccgtggt caacgacgac
      961 tacggccgct ccctccatcc gcccaagcgc ttcgccaggt cggagccggc ggacttccag
     1021 cgcggcgagg tcgagtggag cggcggcaat ctgtacttcg gcgactggga ctacgagttc
     1081 ggcatcgagt accgcgagct cgatagcggg ggcgcctagc ccggccttcc ggcttcgttt
     1141 acgggcttcg tttgcccgcg ggctaccgaa gcggtatcct tcgcactagg gaggtaatga
     1201 aggtgacaaa atcgctcgtg ttcatcgcgt ggttcctctt gcccgccata ccagccgcgc
     1261 tgtacgcggc ctcggcaggc ggattcccgg acgcctatag cgtctcggtc gccctgggcg
     1321 cctacgccta cgcgcttctg gccgctcagc cggccctggc cgccaggccg gccttcatcg
     1381 tcaaggctat cggcgcaaag ggaagcgcca ccctgcacgg cgtcgctccg gtcctggccc
     1441 tcggcctggc cgccgcccac cgggcgctca agctgggcct cggcttcgag gccggtacga
     1501 cccaggccgt catcggcgcg gcctcgttct ggttcctggt cgcggcctcg gtcgcggccg
     1561 ttatcctgct cgccgccttc ggcggcgccc tcggcaaggc gctcaagccg ctcagggcct
     1621 gggtagccga gcggctcggc ctgaactaca aggccgcgag ggccgctcat ggcctggccg
     1681 cgctggccgt cgtcgccctg ttcgtccacc tgtcgctcgc ctcgagctcc gacctatcgt
     1741 tcaacccggc cgggccggcc tggctggccg cctggactct cgtatcgttc ggcgtatacg
     1801 cgcgttcgcg cctgtcggcg aggagcgcgg gaaaggcatc gcgatgaagc gcgtcgtttt
     1861 tatcatggta gcgttgatgg ccgcggcgtc ggccgcctcg gcccaggact tggtccggtc
     1921 gtccgccgcg ccgtccgtcg acgggatagc cgccgcgggc gagtactcca cctggttcga
     1981 gcgctcggag atacgggtgg gcctgtcgct atcggccgac ggctcgacgc tctacgtcgc
     2041 cgtcgacgcg ccgaccgccg gctgggtagc cgtcggcctc gggtcgctca agatggacgg
     2101 cgcctacatg gtgctggcct acgtcgagga ctcgggcacg gtcttcatcg gcgaggagac
     2161 gggctacggg cgctcgcacc ggccgaacgc ggagaagcgg ctgcgagcct cggcggtacg
     2221 cgaggcgaac ggccgcacga ccctcgaact cgccctgccc tccgcgggcc tcgccgacgg
     2281 cgcgagcctg aaggcgctgt tcgcctacgg ccgcagggac gaccggatca ccaagcacgt
     2341 ccgctacatc acggcggagt tgcccatcgt ccgccgctag gagcgcgccg gctagaccct
     2401 ggtgtgcacc cacttgccgc ctcgaaagcg tatagcgatc aatccgaacc gtaccacctg
     2461 gtcggcgagc agggccagcc aggccccgaa caggccgagg ccgaggaggt ggatgaaggc
     2521 gtaggacagg gagggcctga tgatcaggat gccggcggcc agcgatatgg ccggatagag
     2581 cgagtcgccg gccccgcgga gggcgccggc gtagatctgg aacgacgact ggaagggctg
     2641 gatgaaggcg acgagcagca tcacgccgga gctgatggcg atgatcgccg gctcggcggt
     2701 gaacatcgcg acgagggagc gcctgaaaac gaacatcagg acgcccatga cggtcgacac
     2761 gacggcgccc agcttctggc aagcgcccga ggcccgccgc gcggcgtccg ggctgccccg
     2821 cccgagcgcc tggccggcca ggctggtcgc cgctatgccg aaggcctggc cgttgacgaa
     2881 ggatatattc aggatcgaca gcacgatctg atgggcggcg aagacctcgg taccgagcgc
     2941 ggtgatggtg atcgtataga tgacgagccc cgcgcgcagg gccagctgct cggcggccga
     3001 aggcaggcct atcctgacga tgcgcttgag catcgccggg ttcggcacca ggttctccct
     3061 ggtcaggccg aaggacgcga agctgcgctc gcctgagcgc cggcggaaca gcgcgtagac
     3121 ggccatcgcg caggcggcgc agttgcctag caccgtggct atcgccgcgc ccgccacgcc
     3181 gagggccggg aaaccgaatt tgccgtagat caaca
//
LOCUS       DAOPHV010000287         3212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_41652_length_3212_cov_2.770985, whole genome shotgun sequence.
ACCESSION   DAOPHV010000287 DAOPHV010000000
VERSION     DAOPHV010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41652_length_3212_cov_2.770985"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="PLQ29_08130"
     CDS             complement(<1..1404)
                     /locus_tag="PLQ29_08130"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPG86652.1"
                     /translation="MATVTIERLPALSVGASVPLPNGYVDSGYAEDCASLPRDYGTES
                     WEARPEVIEIRRAVFAALDRLEGETSIASRFRGRPVLVKPNLVTVYQDMGMRRGSYPE
                     TTDPRVLDAVVLWLGRFASTIRIVESSGRGAPTRGSFRVSGIDRLARHRGCDLVALEE
                     TPVDRYLLPKASAQREILVPEAFSAVVRGEAAYVSVPKLKTNLYTGVTLGFKNAMGVI
                     PYNLRQRNHHYAINRKLVEMLYLFKPDLVVIDGVVGGEGECPAPVDPVDSRVIIAGDH
                     PVETDRVATRLMGFDPGSIELMRVADELGFGDATGARVLGDAASARFRPADWSLTSER
                     VRRSFPEILVLIGIDRGGLEGKAGPELVEAMQASCRGGCVATTRFAFSMFEAEGVRPR
                     RPVALIIGCGLERDGRRLWFDAEGKGYAEDDILALPMRKAVIGSCTRALAPLATRFVD
                     GCMVLPNAPHVVLHELSG"
     gene            1539..2771
                     /locus_tag="PLQ29_08135"
     CDS             1539..2771
                     /locus_tag="PLQ29_08135"
                     /inference="COORDINATES: protein motif:HMM:NF025608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HPG86653.1"
                     /translation="MGDERNGYMLSGARARRGYDWWWHSLVATSEKTGERRPFFIEYY
                     VINPGFGDAEPVAGQLPANKKAGRKPCYAMIKAGCWGAAAKAQLHNLYPVAAFSASRD
                     GMDVRIGDHVATETRLKGSVSLSAADAAAHPEYMSDSGEMSWDLEAEKILRFSVGWGT
                     SAFFRKLDAFNMYWHVAGMRTRYKGVITFNGERFIVSPDDSFGYQDKNWGRDYTSPWV
                     WLNCNNLRSRKTGKLLERSSLDVGGAQPVAFGISLPRRLLIALHYEGAFYEWNFSKFW
                     TGSKQVFDCPVRDDRVEWNIDAWHGKAKVEIRFSCPRDTMLNVNYEKTPTARSGTTRC
                     GTAATPPARYGCSRGAAANGSRSTSSKASWAAASTENTTARARRLSGRRRREPRRGSR
                     LRRRRRIAPPWSVWTASR"
     gene            complement(2659..3129)
                     /locus_tag="PLQ29_08140"
     CDS             complement(2659..3129)
                     /locus_tag="PLQ29_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPG86654.1"
                     /translation="MKRTDNAPRVDRKTADELLYGSLRAVYHFERSLVERFGLGYQEI
                     YLLQLLRRRETARIGEIADALGVKMFTMSRLVSRLRDRGYVSKAQDSGDRRGILVGLE
                     PRGDELVAAIEKANYELVIGSLSRLTTAEQSAFVVVAENLDEALGVADRTVYEP"
BASE COUNT          562 a         1066 c         1054 g          530 t
ORIGIN      
        1 cccggacagc tcatggagca cgacgtgcgg cgcgttcggc agcaccatgc aaccgtcgac
       61 gaagcgcgtc gccagcggcg cgagcgcgcg cgtacacgag ccgatgacgg ccttgcgcat
      121 cggcaacgcg agtatatcgt cctcggcgta gcccttgccc tcggcgtcga accagagtct
      181 acggccgtcg cgctccaatc cacagccgat tatcagcgcc acgggccgtc tcgggcgcac
      241 gccttccgct tcgaacatcg agaacgcgaa acgtgtggtc gccacgcatc cgccgcggca
      301 cgaggcctgc atcgcctcga cgagctcggg cccggccttg ccctcgagcc cgccgcgatc
      361 gataccgatc agcacgagta tctcggggaa ggatcgacgg acgcgctcgg aggtcaagga
      421 ccaatcggcc ggcctgaatc gcgccgaggc tgcgtcgccc aggacccggg cgcccgtggc
      481 gtccccgaag cccagttcgt cggcgacgcg catcagctcg atcgatccgg ggtcgaagcc
      541 catcagccgc gtcgcgaccc ggtcggtctc caccgggtgg tcgcccgcga tgatcacgcg
      601 cgagtccacc ggatcgaccg gtgccgggca ctcgccctcg ccgccgacga cgccgtcgat
      661 cacgaccagg tccggcttga acaggtatag catctcgacg agcttgcggt tgatggcgta
      721 gtggtggttg cgctggcgca ggttataggg tatgacgccc atcgcgttct tgaagccgag
      781 cgtcacgcca gtatacaggt tcgtcttgag cttaggcacc gagacgtagg ccgcctcccc
      841 gcgtacgacg gcggagaagg cctcgggcac gagtatctcc cgttgcgcgg aggccttggg
      901 tagcaggtag cggtctaccg gcgtctcctc gagcgcgacc aggtcgcagc cgcggtgcct
      961 ggccagccga tctatacccg agaccctgaa ggagccgcgg gtcggggccc cgcgccccga
     1021 cgattcgacg atgcgtatcg tcgacgcgaa gcgtccgagc catagcacca ccgcgtcgag
     1081 cacccgcggg tccgtggtct ccgggtagga cccgcgtcgc attcccatat cctggtagac
     1141 ggtcacgagg ttcggcttga cgaggacggg ccgtccccgg aagcgcgagg ctatggacgt
     1201 ctccccttcg aggcggtcga gcgccgcgaa taccgcccgg cgtatctcga tcacttccgg
     1261 ccgtgcctcc caggactccg tgccatagtc gcggggcagg ctggcgcagt cctccgcgta
     1321 gcccgagtcg acgtagccgt tcggcagcgg caccgaagcg ccgacggata aggccggcaa
     1381 tcgctcgatc gtgacagtcg ccatctgtag cctcccggtc gggaccagcc cggaccatga
     1441 ttatagtata cgctgtacat taaatttgca tagtgcaaat ttatgggcta tcatcgtagc
     1501 cgcgggccgg acacgagccg ccccgctagg aggcaccgat gggcgacgag cgtaacggtt
     1561 acatgctctc tggcgcgcgg gcgcggcgcg ggtacgactg gtggtggcat tctctcgtgg
     1621 ctacgagcga gaagaccggt gagcgcaggc cgttcttcat cgagtactac gtgatcaacc
     1681 ccgggttcgg tgacgccgag ccggtggctg gccagcttcc ggccaacaag aaggccggaa
     1741 ggaagccgtg ctacgcgatg atcaaggctg gctgctgggg cgccgcagcc aaggcccagc
     1801 tccataacct gtaccccgtg gcggcgttct ccgcctcgcg ggacggcatg gacgtgcgca
     1861 tcggcgatca cgtcgccacc gagaccaggc tcaagggctc cgtctcgctg agcgccgccg
     1921 acgccgcggc ccacccggag tacatgtccg actcgggcga gatgagctgg gacctggagg
     1981 ccgagaaaat cctgcgcttc agcgtcggct ggggcacgag cgcctttttc cgcaagctgg
     2041 acgccttcaa tatgtactgg cacgtggcgg gcatgcgcac ccgctacaag ggcgtcatca
     2101 ccttcaacgg cgagcgcttc atcgtctcgc ccgacgactc gttcggctac caggacaaga
     2161 actggggccg cgactatacg agcccctggg tctggctgaa ctgcaacaac ctcaggagcc
     2221 gcaagaccgg gaagctcctg gagcgatcga gcctcgacgt cggcggcgcg cagccagtgg
     2281 ctttcggcat ctccctgccc aggcggctgc tcatcgccct gcactacgag ggggctttct
     2341 acgagtggaa tttctccaag ttctggaccg gctccaagca ggtcttcgac tgcccggtgc
     2401 gcgacgacag ggtggagtgg aacatcgacg cgtggcacgg caaggccaag gtcgagatcc
     2461 gcttctcctg cccgcgcgat accatgctca acgtcaacta tgagaaaacc ccgacggcga
     2521 gaagcggcac aacgcgctgt ggaacggcgg ctacgcctcc ggcacgatac ggctgctcga
     2581 gaggcgcggc ggcgaatggg tcgaggtcga cgtcctcgaa ggcgagctgg gcggctgcga
     2641 gtacggagaa tacgaccgct agggctcgta gactgtccgg tcggcgacgc cgagagcctc
     2701 gtcgaggttc tcggctacga cgacgaaggc ggattgctcc gccgtggtca gtctggacag
     2761 cgagccgatg acgagctcgt agttggcctt ctcgatcgcg gctacgagct cgtcgccgcg
     2821 aggctcgaga ccgacgagta tgccccttcg atcgcccgag tcctgcgcct tactgacgta
     2881 gccgcggtca cgcagccgcg agaccagccg cgacatggtg aacatcttga cgcccagcgc
     2941 gtcggctatc tcgcctatcc tggccgtctc gcgcctgcgc agcagctgca gtaggtatat
     3001 ctcctggtag cccaggccga agcgctcgac caggctacgc tcgaagtgat agacggcgcg
     3061 caggctcccg tacagcagct cgtcggcggt cttcctgtcg acgcgcggag cgttatctgt
     3121 tctcttcatg gttctagtat agcgcctcgc gggggagaat gtgaaacgcg tcgggcccga
     3181 taggacccga cgcgtttcga tctataactt ag
//
LOCUS       DAOPHV010000288         3211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_41659_length_3211_cov_4.385298, whole genome shotgun sequence.
ACCESSION   DAOPHV010000288 DAOPHV010000000
VERSION     DAOPHV010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41659_length_3211_cov_4.385298"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLQ29_08145"
     CDS             complement(<1..252)
                     /locus_tag="PLQ29_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPG86655.1"
                     /translation="MNPIADKADTECACRSLHEGPIAAARAAEEPVSRLLDLTELFKV
                     LGDPSRLRIIRALGAAELCVCDLSSALEMSQSAVSHQLAI"
     gene            422..1528
                     /locus_tag="PLQ29_08150"
     CDS             422..1528
                     /locus_tag="PLQ29_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG86656.1"
                     /translation="MSLAFSLAIVSAALAAFTVVLAILLRRSAATASAATAAASAARA
                     EADARAAEVESLRAVDARLLEELRPVVGAAIAEFRDLLDKAAAVGAKTDSQRETVLAA
                     GDSVSALVRLLSGMVDGLASYAEAFAGSKAAIGALAGGLERMKAEADLLADGAGALLA
                     DSKAGTASLDGAVGAIAEIGEAAKRVRANLGQIAGISARTNLLAMNAAIEAAHAGDSG
                     KGFAVVAGEVRALAELSAKTTKGIEGDMKAMEQAIARGNRAADETKEAFSAIARGVAA
                     SASETGGMAATMDERRRELAAVLPAMDDIAKRLEDILSRAADSAERRIVVEESLGSIR
                     RLADEIREDERRLVEQDMVILAGLEQAEATINRP"
     gene            complement(1525..2736)
                     /locus_tag="PLQ29_08155"
     CDS             complement(1525..2736)
                     /locus_tag="PLQ29_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPG86657.1"
                     /translation="MGIHGTVLAAALAAIAAFAAPVPAAADEFHFAYAVGDKYRVVST
                     VDEDVYINRRYSHSARIVNRIAFEVADARDDGAALLRGDFSTSTRYAGGVAYVTDRIY
                     RSEYWQLPNGRYEIGPAYYMPTVRHVPTFPARDLRPGDTWTAPGEERHDFRDDFGIPD
                     PYIIPIDVRYTYEGPGELDGRPVRLVRAAYTVFVQPARPRSWTIAWPTQIAGYSDQLL
                     YWDPGRGALAAYEERFKFVFELSDGRTVEYRGQAGSEAVEAETMDRPGLERELRDAVE
                     GLDGVTVTSGELGVTISLEDIRFRADSAVLLPSELAKIERIAGLLKGVADRDILVSGH
                     TALAGTEAARLALSTERARAVADQLIRLGAREAERITVIGYGAERPVADNSTEAGMAR
                     NRRVEITILEN"
     gene            complement(2787..>3211)
                     /locus_tag="PLQ29_08160"
     CDS             complement(2787..>3211)
                     /locus_tag="PLQ29_08160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG86658.1"
                     /translation="FSDDRDWLEGVETSLAVNSGSPGYDRDYVERSFAATRRAVEGGA
                     GEWWGAWDGPRCAAQMGLFGYGTTARFKDVETHPGYRRRGVCRTLLYELCERWSASRG
                     PATFVISPEDDDVRRVYEAVGFRYRERVADLCLRPPAA"
BASE COUNT          509 a         1110 c         1093 g          499 t
ORIGIN      
        1 tatggccagc tgatggctga ccgccgactg gctcatctcc aaagccgacg ataggtcgca
       61 gacgcacaac tcggccgccc cgagcgcgcg tatgatacgg agccggctag ggtcgccgag
      121 caccttgaac agctcagtaa gatcgaggag cctggagacc ggctcctcgg cggcccgggc
      181 ggcggctatg gggccttcgt gcaacgaacg gcacgcgcat tcggtatctg ccttgtcggc
      241 tatagggttc atatcagtct ctcctgctaa cacatgaaca tatgaacaaa ggaacatgta
      301 tatatgtact gacttggcgt cgccatggca agcccccgga ttgtcccatg acaaacaaaa
      361 cgctaatctg ctataatttc cgtaccgttc cgaagaatca tcgccatcgg agatgccgat
      421 catgagtctc gccttttcgc tcgctattgt ctcggcggcc ctggccgcgt tcacggtcgt
      481 tcttgccatc ctcttgcgcc gtagcgccgc cacggcctcc gcggcgacgg ccgccgcctc
      541 ggccgcccgc gccgaggccg acgcccgggc ggccgaggtc gagtccctcc gcgccgtcga
      601 tgccaggctc ctcgaggagc tacggccggt cgtcggcgcg gccatagccg agttcaggga
      661 cctcctcgac aaggcggccg cggtcggcgc caagaccgac agtcagcgtg agacggtgct
      721 cgcggccggc gactcggtgt cggccctggt caggctgctg tccggcatgg tcgacggcct
      781 cgcctcgtac gcggaggcct ttgccgggag caaggctgcc atcggggctc tcgccggcgg
      841 tctggagcgg atgaaggctg aggccgacct cctggcggac ggcgccggcg ccctcctcgc
      901 cgactcgaag gccggtaccg cctccctcga tggcgccgtt ggcgcgatcg ccgagatcgg
      961 ggaggcggcc aagcgggtca gggcgaacct cgggcagatt gccggcatct ccgcgcgcac
     1021 gaacctgctg gccatgaacg ccgcgatcga ggcggcgcat gcgggcgact cgggcaaggg
     1081 tttcgcggtc gtggccggcg aggtgcgcgc cctggccgag ctgagcgcca agacgaccaa
     1141 gggcatcgag ggcgacatga aggccatgga gcaggcgata gcgcgcggca acagggccgc
     1201 cgacgagacc aaggaagcct tctctgccat agccaggggc gtggccgcga gcgcctccga
     1261 gaccggcggc atggccgcga ccatggacga gcgcaggcgc gagctggcgg cggtgctacc
     1321 ggcgatggac gacatcgcca agcgcctcga ggacatcctg tcccgggccg ccgacagcgc
     1381 cgaacgccgt atcgtcgtcg aggagagcct cggctcgata cgcaggctgg ccgacgagat
     1441 ccgcgaggac gagcgccgac tcgtcgagca ggacatggtc atcctcgccg ggctcgagca
     1501 ggccgaggcg acgataaacc gaccctagtt ttcgagtatc gttatctcca cccgcctgtt
     1561 gcgggccatg ccggcctcgg tcgaattgtc ggcgaccggc cgctcggccc cataacctat
     1621 caccgtgatg cgctcggcct cgcgggcccc gagccgtatc agctggtcgg ccaccgcccg
     1681 cgcccgctcg gtcgatagcg ctaacctggc ggcctccgtg ccggcgagcg ccgtgtgccc
     1741 gctcacgagt atgtcccggt cggccacgcc cttcaggagc ccggcgatcc gctcgatctt
     1801 ggccagctcc gaaggcagga gcacggccga gtcggcgcgg aagcgtatgt cctccaggct
     1861 gatcgtcacg ccgagctctc cgctcgtcac cgtcacgccg tcgaggcctt ccaccgcgtc
     1921 gcgtagctcg cgctcgaggc cggggcgatc catcgtctcg gcctcgaccg cctcggaacc
     1981 cgcctgcccg cggtactcca cggtccggcc gtcggacagc tcgaagacga acttgaagcg
     2041 ctcctcgtag gcggcgagcg cgccgcggcc gggatcccag tacaggagct ggtcggagta
     2101 cccggctatc tgcgtcggcc aggctatcgt ccaggagcgc ggcctggccg gctggacgaa
     2161 cacggtgtag gccgcgcgga ccagcctgac cggcctgccg tcgagctcgc cgggcccctc
     2221 gtaggtatag cgcacatcga tcgggatgat gtacgggtcg ggtatgccga agtcgtcgcg
     2281 gaagtcgtgg cgctcctcgc cgggagcggt ccaggtgtcg ccgggcctca ggtcgcgggc
     2341 cgggaaggtc ggcacgtgcc tgaccgtcgg catgtagtag gccgggccga tctcgtagcg
     2401 tccgttcggt agctgccagt actcggagcg gtagatgcgg tcggtcacgt aggctacgcc
     2461 gccggcgtag cgcgtgctgg tcgagaagtc gccccggagc agggccgccc cgtcgtccct
     2521 ggcgtccgcc acctcgaagg cgatgcggtt gactatcctg gccgagtggg agtagcgccg
     2581 gttgatgtag acgtcctcgt cgaccgtcga gacgacgcgg tacttgtccc cgaccgcgta
     2641 ggcgaagtgg aattcgtcgg cggcggccgg gaccggagcg gcgaaggccg cgatcgccgc
     2701 cagggcggcc gcgagtaccg tcccgtgaat gcccatgact aatcgcatcg gtataccccc
     2761 cagtatagta tcggtcggaa acctgcctag gccgccggcg gcctcaggca caggtcggcg
     2821 acgcgttccc ggtagcggaa gccgacagcc tcgtagacgc gcctgacatc gtcgtcctcc
     2881 ggcgagatga cgaaggttgc cggcccgcgc gaggctgacc agcgctcgca gagctcgtag
     2941 aggagggtgc ggcatacgcc tcgccttcgg tacccgggat gcgtctccac gtccttgaac
     3001 cgcgccgtcg tgccgtatcc gaacaggccc atctgggcgg cgcaacgggg cccgtcccag
     3061 gcgccccacc attcgcccgc gcctccctcg accgctcgcc tcgtagccgc gaaggaccgc
     3121 tcgacgtagt ccctgtcgta gccgggcgag ccggagttta ccgcgaggct cgtctccact
     3181 ccctccaacc aatcgcgatc gtcagaaaaa g
//
LOCUS       DAOPHV010000289         3202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_41826_length_3202_cov_5.124563, whole genome shotgun sequence.
ACCESSION   DAOPHV010000289 DAOPHV010000000
VERSION     DAOPHV010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41826_length_3202_cov_5.124563"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..1006
                     /locus_tag="PLQ29_08165"
     CDS             377..1006
                     /locus_tag="PLQ29_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPG86659.1"
                     /translation="MHSDTTSITAIAQAPRRRDDRREELFAAALRQYAKHGYKRTRVE
                     DIAAELGLTKGAVYFYAKGKEELYREAISWALGEWCSAIERSAARASSSVERVSILSR
                     SSFAYVEAHHDLRSVIARDPSIFSLDPASDRFASVNDRARGLLESAIRSGVASGEFRS
                     TIDPSAASRFLYQVYITFLIKAFALGEGDELAAQYEAGTDIALMGLAAR"
     gene            1031..1681
                     /locus_tag="PLQ29_08170"
     CDS             1031..1681
                     /locus_tag="PLQ29_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86660.1"
                     /translation="MTEATVRALTGLRDLTTIQWYVIPILAVVFYIYTIEIKKARASG
                     DWNAVIAGAAVFGADFFNESWNGWFMVLSGRSALWTAPGPTALRTMVGWNIEIMFMFS
                     ILGIIWYHALSDARNKRILGVDEKWFFAIGYSVVCVVIECALNAGGHLVWDYRFWERT
                     LIGVPLIFVIGYLWFFAWAILAITRGSTKGKATIAAVPYVAALVLNVAGAALGFKY"
     gene            complement(1671..2876)
                     /locus_tag="PLQ29_08175"
     CDS             complement(1671..2876)
                     /locus_tag="PLQ29_08175"
                     /inference="COORDINATES: protein motif:HMM:NF019174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HPG86661.1"
                     /translation="MSYELTSEHEKILSSLLRFVTVAGVLAYLPGTVVGFLESLWAVV
                     AVNTIAYATVAAVAFIPGVGYRAKLTVLVGACLAVGSVVLVSTGPLGAGYIWLLVGVV
                     LSALFGRKRAVALTIGATMAIMIGWAVALGVGADGRGATPWNVGIIAASLLVICLALS
                     FIIRRLQGSLASAFEDKARLADSLAAELQESHSTKDRLESTLGEMDELLRELQHRVRN
                     NLQTVASLLALDERPPTSRAQGPDEPLERARRRIRALTVANDCFLDGGDGGRADAWGL
                     IRSAAQSADEDHRGCDCRLLRTDRGEPTIDPHAAPLVAILVSDVAASLGSLGRMALVV
                     KPGDGVVVEARCLAPGENPEARVVYRAIASGRVARSAEPEIRLSFIEPDDGSGPGIRL
                     EYREGRVST"
     gene            3038..>3202
                     /locus_tag="PLQ29_08180"
     CDS             3038..>3202
                     /locus_tag="PLQ29_08180"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HPG86662.1"
                     /translation="MQDSSSQGQAVENNGGAKVKQVIGVSSGKGGVGKSTVAVLLAEA
                     LAARGAKVGLL"
BASE COUNT          593 a         1039 c         1017 g          553 t
ORIGIN      
        1 aaccggtacg gctggccgga ggccagacga gcccgcaccg ctacgtgtac aggaaactgg
       61 aaagatacca tagtcggccg ccgaccatcc ggcccgcggg cggtcggcgt cacggatcag
      121 ccggttccgt tcccttggcg gaaccggtac ggctggccgg aggccagacg agcccgcacc
      181 gctacgtgta caggaagctg gaaagatacc atagtcggcc gccgaccatc cggcccgcgg
      241 gcggtcggcg tttcggcaag cccggcttcg cgcgggctga gcctccgttc gaaaccgaac
      301 ggtatggagt gcctgggccg ggttgtttac atagaaacac attgactaat taaactttcc
      361 gtttaattat ttaataatgc actcggatac tactagtatt accgccatcg cccaggctcc
      421 gaggcggcgc gacgataggc gcgaggaact cttcgccgcg gcccttcggc agtacgccaa
      481 gcatggctac aagcgaaccc gcgtcgagga catagccgcc gagctcggtc tgaccaaggg
      541 cgcggtctat ttctacgcga agggcaagga agagctctat cgcgaggcca tatcatgggc
      601 gctcggcgag tggtgctcgg ccatagagcg gtcggccgct cgcgcctcct cctccgtgga
      661 gcgcgtatcg atactatcac gatcgtcgtt cgcctacgtc gaggctcatc acgacctccg
      721 ctccgtcata gcgcgcgatc cttccatctt tagcctcgac ccggcgtcgg accgcttcgc
      781 ctcggtcaac gaccgcgcgc gcggcctgct cgagagcgct attcgctcgg gagtggcatc
      841 gggcgaattc aggtcgacga tagacccctc cgccgcctcg cgcttcctgt accaggtcta
      901 cataaccttc ctcatcaagg ccttcgcgct cggcgagggc gacgagctgg ccgcccaata
      961 cgaagcgggc accgatatcg cgctcatggg ccttgcggcc cgatagatac gctcttcaaa
     1021 ggagaatcga atgaccgagg ctacggtacg ggccctgacg ggcctgcgag acttgacgac
     1081 gatccagtgg tacgtgattc ctatcctggc cgtcgtcttt tacatctaca cgatcgagat
     1141 caaaaaggcg cgcgcgagcg gcgactggaa cgccgtcatc gccggcgcgg ccgtcttcgg
     1201 cgccgacttc ttcaacgaga gctggaacgg ctggttcatg gtcctgtcgg ggaggagcgc
     1261 cctctggacc gcccccgggc cgacagcgct caggacgatg gtagggtgga acattgagat
     1321 catgttcatg ttctcgatcc tcggcatcat ctggtaccac gcgctatcgg acgcccgaaa
     1381 caagaggata ctcggggtcg acgagaaatg gttcttcgct atcggctact ctgtggtctg
     1441 cgtcgtcatc gagtgcgccc tcaacgccgg cggacacctg gtctgggact atcgcttctg
     1501 ggaacggacg ctgatcggcg taccactcat cttcgtcatc ggctacctgt ggttcttcgc
     1561 atgggccatc ctggccatca cgcgcggttc gaccaagggc aaggcgacta tcgcggccgt
     1621 tccttacgtc gctgcgctag tcttgaacgt ggccggcgcg gctctaggct tcaagtactg
     1681 acccgaccct cgcggtattc caggcgtatg cccggccccg agccgtcatc gggctcgatg
     1741 aaagagagcc tgatctcggg ctcggcgctc cgtgcgaccc ggcccgaggc gatggcgcga
     1801 tagacgaccc gcgcctcggg gttctctccc ggggcgaggc agcgcgcctc gacgacgacg
     1861 ccgtcgccag gcttgacgac caaagccatc ctgccgagcg agccgaggct agccgcgacg
     1921 tcgctgacga gtatggccac gagcggagcc gcgtgcggat cgatcgtcgg ctcgccccga
     1981 tccgtacgga gcagcctaca atcgcagccg cgatggtcct cgtcggccga ctgggccgcc
     2041 gagcggatca ggccccaggc atcggccctg ccgccgtccc cgccgtcgag gaagcagtcg
     2101 ttggctaccg tcagagcgcg tattctgcgc cgagcgcgct ccagcggctc gtcgggaccc
     2161 tgggcccgac tggtcggcgg gcgctcgtcg agcgccagaa ggctagcgac ggtctgcagg
     2221 ttattcctga cacggtgctg gagttcccgg aggagctcgt ccatctcgcc gagggtcgat
     2281 tcgagcctat ccttggtgct atgggactcc tggagctcgg ccgccaggct gtcggccagc
     2341 cgggccttat cctcgaaggc cgacgccagg ctaccctgta ggcgcctgat gatgaacgac
     2401 agggcgaggc agatgacgag gaggctcgcg gcgataatgc cgacgttcca gggtgtggcg
     2461 cctcgtccgt cggcgccgac cccaagggcg acagcccagc cgatcatgat ggccatcgta
     2521 gcccctatgg tcagcgccac ggctcgtttc cggccgaaca aggccgacag caccacgccg
     2581 acgagcagcc agatgtagcc agccccgagc ggcccggtag atacgagcac gaccgacccg
     2641 acggcgaggc aagccccgac gaggacggtc agcttagccc tgtagccgac gcccgggatg
     2701 aaggcgacgg ccgcgactgt ggcgtaggct atcgtattga ccgccacgac ggcccagagg
     2761 ctttcgagga aaccgacgac cgtaccgggc aggtacgcca ggactcccgc tacggtaacg
     2821 aaacgtagga gcgacgatag gatcttctca tgctcggacg tcagctcata gctcacgcat
     2881 gaagagtatt cgactatcca gcccgcgtca aatggagctt tcctggagat ataagcatat
     2941 gcccacttaa catggatcgc ccaggcgtat ataataggcc tcggataaat agatccaatc
     3001 accggcgccc gcgaggcgcc gtttcaggaa ggttcgcatg caggattcaa gctctcaggg
     3061 ccaggccgtg gagaataacg gcggagccaa ggtcaagcag gtcattggcg tctcgagcgg
     3121 caagggcggc gtcggcaagt cgaccgtcgc ggtgctgctc gccgaagcgc tcgccgctcg
     3181 cggcgcgaag gtcggcctcc tc
//
LOCUS       DAOPHV010000290         3197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_41906_length_3197_cov_4.904519, whole genome shotgun sequence.
ACCESSION   DAOPHV010000290 DAOPHV010000000
VERSION     DAOPHV010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41906_length_3197_cov_4.904519"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLQ29_08185"
     CDS             <1..608
                     /locus_tag="PLQ29_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPG86663.1"
                     /translation="FTAALAGPGWGADERNAARLESLWDAALPLALDADALGLLATSC
                     LARRCSPLLLTPHPGEFAPLAAVAAGAQPDDPAALELAARRTRFDTAAVVAETAAYFG
                     AVVALKGSVTWIGDPDGRLAVWDGREPALATAGSGDVLVGLAAGFLARGASAWDAAKA
                     AVIAHGLAGRALARRGFFEADALLPEVARLSYGRGINGNQG"
     gene            592..1119
                     /locus_tag="PLQ29_08190"
     CDS             592..1119
                     /locus_tag="PLQ29_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86664.1"
                     /translation="METKDKGQAVYYYDRAKRMEKAGPYARFMAEHYGAKRPGILRSL
                     TATRPLKSLFLALMFMLVAVLVVGYVQGAKNSGSVAGSRLVSTAMWFDGHVYVTVKRK
                     PTFFGRDAGPQDRLAIEIAAGDGSAHAVAVLQAADDETRLRFEAASKPEAVAVLATAG
                     SGDDASRLEMLVKVE"
     gene            1206..1616
                     /locus_tag="PLQ29_08195"
     CDS             1206..1616
                     /locus_tag="PLQ29_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86665.1"
                     /translation="MVQFYLLSVLMNIVSGYALVSTDAEPRGTKMDGIREFLRDSTIR
                     LVLGILCASVGFFKLLTVMRGDVPVVGDLVPSLAGLAAGFSLLLEFYKSNSNVTTPAL
                     ERLDAIFVAQRRVVGIVAIVSGFVHFLFGNVLFL"
     gene            1617..2012
                     /locus_tag="PLQ29_08200"
     CDS             1617..2012
                     /locus_tag="PLQ29_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPG86666.1"
                     /translation="MRSIAGVSVRDGRVFVARRSPGGSMGGTWEFPGGKCEPGESDAE
                     AVVREYDEEFGLRVVPGPTIGESGFVNGVKPYELAAVMVDFDGEPPELREHDEYRWVD
                     AEALLALELSDSDRSLLAFVLPLLVDRVE"
     gene            complement(1989..2222)
                     /gene="xseB"
                     /locus_tag="PLQ29_08205"
     CDS             complement(1989..2222)
                     /gene="xseB"
                     /locus_tag="PLQ29_08205"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPG86667.1"
                     /translation="MKSFEERLQRLEELSEKIREPDLALEDAVAAFEEGVKLSKGLEK
                     DLDRIEARVEILLNEPSPPDGKPELGLFDPVDQ"
     gene            complement(2219..>3197)
                     /gene="xseA"
                     /locus_tag="PLQ29_08210"
     CDS             complement(2219..>3197)
                     /gene="xseA"
                     /locus_tag="PLQ29_08210"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPG86668.1"
                     /translation="LVRARGALGVYAARGQYQLVVDTMQKAGEGDILAMLERRKRQLA
                     AEGLFDADRKPPLPAFPARVAVVTSPTGAALRDILSVLGRRNSGIRVTVLPAAVQGDQ
                     APEAIVRQIEAANAFGLGDVIIVGRGGGSLEDLLAFSDEAVVRAVAASRIPVISAVGH
                     EIDWCLCDYAASLRAPTPSAAAELVSESAQAIADRVTIAADSLALGIRARLERARAIV
                     DSFSSEQLELRLERALQPSRQRFDDAKEALVRGTRDACLAARHRLSSADAVLRASDPL
                     AILARGFAVVRKPGEPRALRSAAELAAGDGVSIQFSSGVADAAIVEVRA"
BASE COUNT          455 a         1075 c         1118 g          549 t
ORIGIN      
        1 gcttcaccgc ggcgctggcc gggccgggct ggggcgccga cgagcggaac gccgcgcggc
       61 tcgagtcgct gtgggacgcg gccctgccgc tggccctgga cgccgacgcg ctcgggctgc
      121 tcgcgacttc ctgcttggcg cggcgctgtt cgccgctact cctgactcct catcctggcg
      181 agttcgcccc gctggcggcc gtcgccgcgg gcgcccagcc cgatgacccg gcggcccttg
      241 agctggcggc gcgtcggacc cgtttcgaca cggccgccgt ggtcgccgag acggcggcgt
      301 atttcggggc agtcgtcgct ctcaaggggt ccgtaacctg gataggcgac cccgacggca
      361 ggctggcggt ctgggacggc cgcgagcctg cactagcgac cgccggctcc ggtgacgtgc
      421 tcgtggggct agccgcgggc tttctggctc ggggggcttc cgcctgggac gcggccaagg
      481 ccgcggtcat cgcccacggc ctagccggcc gcgccctggc gcgccgcggg ttcttcgagg
      541 ccgacgccct cttgcccgag gtcgccaggc tgtcatacgg aaggggaatt aatggaaacc
      601 aaggataagg gccaggcagt ctactactac gaccgggcta aacgtatgga gaaggccggg
      661 ccgtatgccc gcttcatggc cgagcactat ggagccaagc gcccggggat actgcgctcg
      721 ttgacggcga ccaggccgct caagtcgctg ttcctggcgc tcatgttcat gctcgtcgcg
      781 gtactcgtcg tcggctatgt ccagggggcg aagaactcgg gctccgtcgc cggctcgcgt
      841 ctggtctcga cggccatgtg gttcgacggc cacgtctacg ttaccgtcaa gcggaagccg
      901 accttcttcg ggcgggacgc cgggccgcag gataggctgg cgatagagat agccgcgggg
      961 gacggctcgg ctcacgctgt agcggtactg caggcggcag acgatgagac gcggctgcgt
     1021 ttcgaggccg ccagcaagcc ggaagcggtg gccgtgctcg cgaccgccgg aagcggcgat
     1081 gacgcgtcga ggctggagat gctggtcaag gtcgagtgag ttcgagtgag gtcggtccgg
     1141 cgtcgccttg accgctcccg ggcttttcat tatggtacct aacgaggctg aagggagaag
     1201 tcggcatggt tcagttctat ctcttgagtg tcctgatgaa catcgtctcg ggctacgctc
     1261 tggtctcgac cgacgccgaa ccgcggggta ccaaaatgga cggtatccgc gagttcctcc
     1321 gcgacagcac catccgcctc gtcctgggga tactgtgcgc ctcggtcggt ttctttaaac
     1381 tactgaccgt catgcgcggc gacgtccccg tcgtcggcga cctcgtgccg tcgttggccg
     1441 gtctcgcggc cggtttctcc ctgttgctcg aattctacaa gagcaactcg aatgtgacga
     1501 ccccggccct cgagcggctg gacgccatct tcgtcgcgca gcgccgtgtt gtcggcatcg
     1561 tggccatcgt cagcggcttc gttcattttc tgttcgggaa cgtactcttc ctataaatgc
     1621 ggtcgatagc cggcgtctcc gttcgcgatg gccgcgtttt cgtagcccgc aggtcgccgg
     1681 gaggctccat gggaggcacc tgggagttcc cgggcggtaa atgcgaacca ggcgagagcg
     1741 acgcggaggc tgtggtcagg gagtacgacg aggaattcgg cctgcgcgtc gtgccggggc
     1801 cgacgatagg cgagtccggc ttcgtcaacg gcgtcaagcc atacgagctg gccgccgtca
     1861 tggtcgactt cgacggcgag ccgcccgagc tccgcgagca cgacgagtat cgctgggtgg
     1921 acgccgaggc gctcctggcg ctcgagctgt ccgactccga ccgctccctg ctcgcgttcg
     1981 tgctaccgct attggtcgac cgggtcgaat aagccgagct cgggcttgcc atcaggcggc
     2041 gatggctcgt tgagcagtat ctccacccgg gcttcgatgc ggtcgagatc cttctcgagt
     2101 cccttggaca gcttgacgcc ttcctcgaac gcggccaccg cgtcctccag ggccaggtcc
     2161 ggctcgcgaa tcttctccga cagctcctcg agcctttgta gccgttcctc gaaactcttc
     2221 atgcccgtac ctccacgatg gcggcgtccg cgacgcctga cgagaactgg atcgatacgc
     2281 cgtccccggc cgccagctcg gcggcgctcc tcagcgcgcg cggttcgcct ggcttcctga
     2341 ccacggcgaa acccctggcc aggatggcca gggggtcgga ggcgcgcagt acggcgtcgg
     2401 ccgacgacag gcggtgcctg gcggcgaggc acgcgtcgcg agtgccgcgt accagcgctt
     2461 ccttggcgtc gtcgaagcgc tggcgcgacg gctggagggc gcgctccagc ctcagctcca
     2521 gctgttccga cgagaacgag tcgacgatgg cccgggcccg ctcgagcctg gcgcgtatgc
     2581 cgagcgccag cgagtcggcc gcgatcgtca cccgatcggc gatagcctgg gcgctctcgg
     2641 ataccagctc ggcggccgcg ctcggggtcg gtgcccggag cgacgcggcg tagtcgcaga
     2701 ggcaccagtc tatctcgtgg ccgacggcgc tgatcaccgg tatcctcgac gccgccacgg
     2761 cgcgcacgac cgcctcgtcg gagaaggcga gcaggtcctc gagagagccg ccgccgcggc
     2821 ctacgatgat cacgtcgccg agaccgaagg cgttggccgc ctcgatctgc ctgacgatgg
     2881 cctccggcgc ctggtcgccc tgcacggccg cgggtagcac ggtgacgcgg atgcccgagt
     2941 tgcggcgtcc gagcaccgaa aggatgtcgc ggagcgcggc gccggtcggc gaggtgacca
     3001 cggccacccg ggccgggaag gccggcaggg gcggcttgcg gtcggcgtcg aacagtccct
     3061 cggcagccaa ctgccgtttg cgcctctcga gcattgccag gatgtcaccc tcgccggctt
     3121 tctgcatcgt gtcgacgacg agctggtact ggccgcgagc cgcgtagacg ccgagcgcgc
     3181 ctcgcgccct gaccaag
//
LOCUS       DAOPHV010000291         3179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_42237_length_3179_cov_5.673496, whole genome shotgun sequence.
ACCESSION   DAOPHV010000291 DAOPHV010000000
VERSION     DAOPHV010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42237_length_3179_cov_5.673496"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1757)
                     /locus_tag="PLQ29_08215"
     CDS             complement(<1..1757)
                     /locus_tag="PLQ29_08215"
                     /inference="COORDINATES: protein motif:HMM:NF014235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HPG86669.1"
                     /translation="MSDVIRNCADEPEELIPVPLKILGSRKQLPPGGRIKLYDFRRPD
                     KFSREQLRTMQILAETFGRLASTTLSAALRLPCELSVDLVDQMTYDEYLAPIEGQSTL
                     AVVSMEPLKGQAVVHIDAAGSDALVERVFGSRLIPVAVVGEPGASERPRCAGGLTDIE
                     AAQLERLLAALATDLGQAWGFVPGGLEPALAAIETEARFCQIVPPTEMIVITACSLSV
                     GPVKGRLTVVYPFLLLEPILHALSAKYWYERRGDEVNPRSLAVARRATMPAELIVDAG
                     APPVSLLRSLRKGSEIELPDDDEGRAWLRLGGARVAELSGLRADGDSLSADFAGRPTD
                     AGASKGAANPMAALAAELKAGLAAVSSGVSEAVSSMARRIDELKGGQEDLSDRVLFGQ
                     AESGGRSPGKPFSSLAGVPAEPFALFLSGERPQVCALVLSFLDDALAARLLSLLPEAV
                     RPEVARRIATMDWVTPQALSETERILGKKLSAIGRSGFEADGVKKVVGILNFSPRETE
                     RSVITALDAIDPELSESIKRSMFVFEDIIILDDESIAAVLERADEKDFVVAMKPMKDD
                     ERRRLLERLPAGSRERVGAA"
     gene            complement(1754..1999)
                     /locus_tag="PLQ29_08220"
     CDS             complement(1754..1999)
                     /locus_tag="PLQ29_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HPG86670.1"
                     /translation="MRTGTVEDAMVRVQVVLGGRSLSVRDLSTVAKGSIIELDSIAGE
                     PVDLVAAGEVVAKGEVVIIDENFGIRVTELAERGASS"
     gene            complement(1996..2271)
                     /locus_tag="PLQ29_08225"
     CDS             complement(1996..2271)
                     /locus_tag="PLQ29_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86671.1"
                     /translation="MEARDNSGARTAEVGDLRLDACVVVASGTMSAEDADRARDSGWL
                     AMKAPEGGAAYLEVGGVVVAEGRLRKRRGKNAFVVTRTYQESGEVRS"
     gene            complement(2305..2832)
                     /locus_tag="PLQ29_08230"
     CDS             complement(2305..2832)
                     /locus_tag="PLQ29_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86672.1"
                     /translation="MERNRSIMKDAFINEKEFAPLDDLYLLLKAMQGTLKMEIDGADY
                     IVGREQLYYVFMHETMHAFIGKAAPWIYKLNEDETDFIDESAARILIDDLLGQLGLFD
                     KVRLYYENHVNHRKELNYYGYDLSPEHYDEMEKRYRDAFAATKDIDSFCRYLSGRYHE
                     LGMKRRDGFSYKMNE"
     gene            complement(2936..>3179)
                     /locus_tag="PLQ29_08235"
     CDS             complement(2936..>3179)
                     /locus_tag="PLQ29_08235"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPG86673.1"
                     /translation="RCATNASDFSRRFKERAGCPLFEFINRQRVARACALLKSSDLSI
                     IEISEAVGYNSLSFFNRYFLRIVGLSPREYRSSGPR"
BASE COUNT          515 a         1049 c         1039 g          576 t
ORIGIN      
        1 gccgccccga cccgctcgcg gctcccggcc ggcaggcgct cgagaaggcg gcgccgctcg
       61 tcgtccttca tcggtttcat ggcgacgacg aagtccttct cgtcggcccg ctcgagcacg
      121 gccgctatcg actcgtcgtc gaggatgatg atgtcctcga agacgaacat gctgcgcttg
      181 atcgactcgg acagctcggg gtcgatcgcg tccagggcgg tgatgaccga tcgctccgtc
      241 tcgcgcggcg agaagttcag gatgccgacg accttcttga cgccgtcggc ctcgaagccg
      301 gagcggccga tggccgacag cttcttgccg aggatgcgtt ccgtctccga tagcgcctgc
      361 ggcgtcaccc agtccatcgt cgcgatgcgg cgcgccacct cgggccggac cgcctcgggc
      421 agtagcgaca ggagccgggc cgccagggcg tcgtccagga acgacaggac gagcgcgcag
      481 acctggggac gttctccgga caggaacagc gcgaagggct cggccggcac gccggcgagc
      541 gacgagaaag gcttgcccgg cgagcggccg cccgactcgg cctggccgaa cagcacgcgg
      601 tccgacaggt cctcctggcc gcccttgagc tcgtcgatgc ggcgggccat gctggagacg
      661 gcctcggaaa cgcctgagga gacggccgcg aggccggcct tgagctcggc ggccagggcg
      721 gccatcgggt tggccgcgcc cttggaggca ccggcgtcgg tcggccgccc ggcgaagtcg
      781 gccgacagcg agtcgccgtc ggcgcgtagg cccgacagct cggctacgcg ggctccgccg
      841 aggcgcaacc aggcccgccc ctcgtcatcg tcgggcagct cgatctcgga gcccttgcgt
      901 aacgagcgga ggaggctgac cggcggggcc cccgcgtcga cgatcagctc ggccggcatc
      961 gtcgcccgcc tggccaccgc caggctcctg ggattgacct cgtcgccgcg ccgctcgtac
     1021 cagtacttgg cggacagcgc gtgcaggatt ggctcgagga gcaggaaggg atacacgacc
     1081 gtcaacctgc ccttgaccgg cccgaccgat agcgagcagg ccgtgatcac gatcatctcg
     1141 gtcggcggca cgatctggca gaagcgcgcc tcggtctcga tcgcggctag ggcaggctcg
     1201 aggccgccgg gcacgaagcc ccaggcctgg ccgaggtcgg tcgcgagcgc cgcgaggagc
     1261 cgctcgagtt gggccgcctc gatgtcggtg aggccgccag cgcagcgcgg cctctcgctc
     1321 gcgccgggtt cgccgacaac ggcgacggga atcagccgcg agccgaagac gcgctcgacg
     1381 agggcgtccg agccggccgc gtcgatatgg acgacagcct ggcccttgag cggctccatc
     1441 gagacgacgg cgagcgtcga ctggccttcg ataggtgcca ggtactcgtc ataggtcatc
     1501 tggtcgacca ggtcgaccga cagctcgcag ggcagcctga gcgccgccga cagcgtggtc
     1561 gaggcgagcc ggccgaaggt ctccgcaaga atctgcatgg tccgcagctg ctcgcgggag
     1621 aacttgtcgg ggcgcctgaa atcgtagagc ttgatcctgc cgcccggagg aagctgtttt
     1681 ctagaaccca gtatttttaa cggcacgggg atgagttcct ccggctcatc ggcgcagttc
     1741 ctgatcacgt cgctcatgac gaggccccgc gctcggccaa ttcggtgacg cggatgccga
     1801 agttctcgtc gatgatgacg acctcgccct tggcgacgac ctcgccggcc gcgacgaggt
     1861 ccaccggctc gccggcgatc gagtcgagtt cgatgatcga gcccttggcg acggtggaca
     1921 ggtcgcgcac cgataggctc cgtccgccca gtaccacctg taccctgacc attgcgtcct
     1981 ccacggttcc cgtcctcatg atcgtacctc tcccgattcc tgataggttc gcgttacgac
     2041 gaacgcgttc ttgccgcgcc gtttccgtag ccggccctcc gctaccacta cgccgcccac
     2101 ctcgaggtag gccgcgccgc cttccggcgc cttcatcgcg agccatcccg agtccctggc
     2161 gcggtcagcg tcctccgcgc tcatcgtccc cgaggcgacg acgacgcagg cgtcgaggcg
     2221 taggtccccg acctcggccg ttcgcgcgcc cgaattatcc cgtgcttcca tggcaacctc
     2281 ccccgttcag ctcaatcgtc ccgttcactc gttcatcttg tacgagaatc cgtcccgtct
     2341 cttcatgccc aattcgtgat accgcccgct caagtatctg cagaagctgt caatatcctt
     2401 cgtcgcggcg aacgcgtcgc ggtatctctt ttccatctcg tcgtagtgtt ccggagaaag
     2461 gtcgtatccg tagtagttca gttctttgcg atggtttacg tgattttcgt agtacagcct
     2521 gactttgtcg aagagtccca gttggccgag aaggtcgtcg atgagtatcc tcgccgccga
     2581 ctcgtctatg aagtctgtct cgtcttcgtt cagcttgtag atccatggcg cggccttgcc
     2641 gatgaaggcg tgcatcgtct cgtgcatgaa gacgtagtac agctgttccc ggccgacgat
     2701 ataatccgcg ccgtcgattt ccatcttaag cgtgccctgc atcgccttga ggagcaggta
     2761 caggtcgtcc agtggagcga attctttttc gtttataaaa gcgtctttca taattgatcg
     2821 gttcctctcc atgagcggta atagtatgtc tgaaatgata tggcggttct gcggcgcggt
     2881 ctcacactat cttgagttcg gcgcggcggg aaccggcact atcttgagga agcgcctatc
     2941 gcgggccgga cgatcggtac tcgcgcgggg acaggccgac gatgcggagg aagtagcggt
     3001 tgaagaacga caggctgttg taaccgaccg cttccgatat ctcgatgatc gacaggtcgg
     3061 atgacttgag gagggcgcag gcgcgggcga cgcgctggcg gttgatgaac tcgaacaggg
     3121 ggcagccggc gcgctccttg aagcggcgcg agaagtcgct cgcgttcgtg gcgcacctg
//
LOCUS       DAOPHV010000292         3175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_42317_length_3175_cov_3.285897, whole genome shotgun sequence.
ACCESSION   DAOPHV010000292 DAOPHV010000000
VERSION     DAOPHV010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42317_length_3175_cov_3.285897"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLQ29_08240"
     CDS             complement(<1..651)
                     /locus_tag="PLQ29_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86674.1"
                     /translation="MKRIVLMSCISAILSVAAAFPQGVRTVVSSVVSLSSEEPGGAVV
                     SLRYNEAVAVLFPADPTFIQGVEFELRIPKAFQGAESSIAWSLYSGIAPTPTSGRFDY
                     EARLISTQPLPQRVSMSLILPIIASHGIKGGPFASLIPAIAGLADFPLMFKLSPIGKG
                     LLPAMESAEFKLTIRPVLRDEGGIRVSVTTIDGAEAQGVSVFIDDRMAENPGRPLPG"
     gene            complement(648..1619)
                     /locus_tag="PLQ29_08245"
     CDS             complement(648..1619)
                     /locus_tag="PLQ29_08245"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPG86675.1"
                     /translation="MTASSSRQLSAATILSVLAIVAVPSILAVVPASAQTPEARSAYD
                     YPLIRSLGPRDVVYVQLQDALAQSYRAERTASPYPDLFICRWVAAGGEDLFAVAARLN
                     VPYDTLATLNGLSRPRPFEPGEAVLVPSIAGLFVPSAPASDFEAILKAGRSMGPSGDD
                     SVSVWRSGGAVVLGFLPGARLNATERSFFLNVGFRMPLPTGLLTSRYGYRRSPIDGHD
                     RMHEGVDLAAPLGSDVVAARDGTVLQTGNDSALGLFVVLDHGGGLSTVYGHLSAVDVV
                     LNQTVRSGTIIGAVGSTGLSTGPHLHFEIRLSGQAKDPSRYLPGLKP"
     gene            complement(1616..1828)
                     /locus_tag="PLQ29_08250"
     CDS             complement(1616..1828)
                     /locus_tag="PLQ29_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86676.1"
                     /translation="MSGAALGLVGAAAVAAAAAAVVWPLWYLATEHTGLYTLAASVAL
                     VAGVAWSIAARLRRRRGAAPVRPARE"
     gene            complement(1900..2652)
                     /locus_tag="PLQ29_08255"
     CDS             complement(1900..2652)
                     /locus_tag="PLQ29_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86677.1"
                     /translation="MTPTKYVFVLGGDDPVILHPYDDPETFASAEPGSVVGRYGSGAE
                     PRGADGIRTALYAAMERGTRRYFLNRGYYLRLAMTASAFVAVYLFLSIVVRDPIPLID
                     ELLLGSLSAAAVFFASERKALSSPRHLDAVLRLRREIDGALFSESRVVDLLEAWRDEA
                     LALGPAAFYTIGQGSTLGDDESAEAEALCAALARRWRHKPVVAELYAAIEGGQVPGYL
                     LDKTVRKLGLEEGSLAIAYMRLIPLALRNGTV"
     gene            complement(2686..>3175)
                     /locus_tag="PLQ29_08260"
     CDS             complement(2686..>3175)
                     /locus_tag="PLQ29_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86678.1"
                     /translation="EGYRDEDGFLELDDSSFEQQVFQRIAIAESQEEELGDEITMLTD
                     EQRARLEELQAPELEDDEAVDLEPLVGSTYRQIISAISYHRVEWPGATVEELPAVADG
                     EPIDVMPLESVSREDADAPGEAIVMVDGVYTLNRDAVSNAVPEDQSLKALAESVLTHR
                     GA"
BASE COUNT          591 a         1031 c         1063 g          490 t
ORIGIN      
        1 gccaggaagg ggccgaccag gattctcggc catcctgtcg tctatgaaca ccgagacgcc
       61 ttgagcctcg gcgccatcga tggtcgttac gctgaccctg atgccgccct cgtcgcggag
      121 gaccggcctg atggtcagct tgaactcagc ggattccata gccggcaaga gccccttgcc
      181 gatgggagag agtttgaaca tgagcgggaa atcggccaga ccggctatgg ccggtatcag
      241 gctggcgaac gggccgccct tgatgccgtg agaggctatg atcggcagga tcaagctcat
      301 cgaaacccgc tgcggcaagg gctgggtgga tatcagccgg gcttcgtagt cgaaacggcc
      361 ggacgtcgga gtcggagcta tgccggagta cagcgaccag gcgatcgacg actcggctcc
      421 ctggaaggcc ttgggtatgc gcagctcgaa ttcgacgccc tggatgaacg taggatcggc
      481 cggaaacaac acggccaccg cctcgttata gcgcagggat acgacggcgc cgcccggttc
      541 ctccgacgac agcgagacga ccgacgacac gacggtacgg actccctgcg ggaacgccgc
      601 cgcgacggag aggatggccg agatgcacga catcaggacg atacgcttca tggcttcaac
      661 ccgggcaggt acctggaggg atccttggcc tggccgctca gccgtatctc gaaatggaga
      721 tgggggccgg tggataggcc ggtcgaaccg accgcgccga ttattgtacc cgatcgcacc
      781 gtttggttca acacgacgtc aacggccgat aggtggccgt agaccgtgga taatccgccg
      841 ccgtggtcga gtacgacgaa cagaccgagc gcagagtcgt tgccggtctg cagtacggtt
      901 ccgtcacggg cggcgacgac gtcggatcct agcggcgctg cgaggtcgac gccctcgtgc
      961 atacggtcgt gcccgtctat cgggcttcgc ctatagccgt agcggctcgt tagaagcccc
     1021 gtcggcaggg gcatgcggaa gcctacgttc aggaagaacg atcgctcggt cgcgttgagc
     1081 ctggccccgg gcaggaagcc caagacgact gcgccgccgc tcctccagac cgataccgag
     1141 tcgtcgccgg acggtcccat cgaccttccg gccttcaaga tggcctcgaa atcgctcgcc
     1201 ggcgcagagg gcacgaacaa cccggctatc gacggcacga ggacggcctc gcccggctcg
     1261 aacggtcgcg gccggctcaa cccgttaagc gtggctaggg tgtcgtatgg tacattgagc
     1321 ctggcggcca ccgcgaagag atcctccccc ccggcggcca cccaacggca gatgaacagg
     1381 tcggggtacg gcgaggcggt ccgctcggcg cgatacgact gggccagcgc gtcctgaagc
     1441 tggacataca ctacgtcgcg cggcccaagg ctacggatga gcggataatc gtaggcagag
     1501 cgcgcctccg gagtctgggc gctagccggg acgaccgcga ggatcgaggg gacagcgacg
     1561 atcgcgagga ccgagaggat cgtcgcggcg gacagctggc gcgacgacga cgcggtcact
     1621 cgcgcgccgg ccgaacggga gccgctcccc tgcgccgacg cagcctcgcg gcgatcgacc
     1681 acgcgacacc ggcgaccaag gctaccgagg cggccagggt atagaggccg gtatgctccg
     1741 tagccaagta ccagagcggc cagaccacgg cggcggcggc ggcggcgact gccgcggcgc
     1801 cgacgagccc gagcgcggct ccgctcaagg ctccccttcg aggaccgcga gccttccgaa
     1861 cgcccgagat tccagcagac tcgtcgcgcc gagtgggact catacggttc cgttcctgag
     1921 cgccaggggt atgagccgca tatacgcgat agccaacgag ccttcctcga ggcccagttt
     1981 ccgtaccgtc ttgtccaaca agtagcccgg aacctgcccg ccctcgatgg cggcgtacaa
     2041 ttctgccacg accggcttat gtctccatcg tcgcgcgagg gcggcgcaga gcgcctcagc
     2101 ctcggccgac tcgtcgtcgc ccagggtcga gccctgaccg atcgtataga aggcagccgg
     2161 gccgagggca agcgcctcgt cccgccaggc ctcgagcagg tcgacgacac ggctctcgga
     2221 gaaaagagcg ccgtcgatct ccctccggag ccgcagcaca gcgtcgaggt ggcgtggcga
     2281 cgatagggcc ttgcgctcgc tggcgaaaaa cacggccgcc gcagacaacg agccgagcag
     2341 tagctcgtcg atcagcggta tcgggtcgcg tacgactatg gacaggaaca gatagaccgc
     2401 gacgaaggcc gacgcggtca tggcaagccg taggtaatag cccctgttca ggaaatagcg
     2461 cctcgtgcct cgctccatgg ccgcgtacag ggcggtacgg atgccgtcgg cgccgcgcgg
     2521 ctccgcaccg gagccgtagc ggccaacgac ggaccccggc tcggccgaag cgaaggtctc
     2581 gggatcgtcg tagggatgga gtatgacggg atcatcgcct ccgagcacga agacatattt
     2641 cgttggggtc atattgaaga aagtacgatc ggccggtcgg ccatgtcaag cgccgcgatg
     2701 cgtcagcacc gactcggcca aggctttcag cgactggtcc tcgggtaccg cgttcgatac
     2761 ggcgtcccga ttgagcgtgt acacgccgtc gaccatcacg atcgcttcgc cgggcgcatc
     2821 ggcgtcctcc cgggagaccg actcgagcgg catcacgtcg atcggctcgc cgtcggctac
     2881 cgcgggcaac tcctcgacgg tagctccggg ccactcgacc ctatggtagg atatggcgct
     2941 gatgatctgt cgataggtag agccgacgag cggttcgagg tcgaccgctt cgtcgtcctc
     3001 gagctcgggg gcctgaagct cctccaggcg cgcccgctgc tcgtcggtca gcatcgttat
     3061 ctcgtcgccg agctcctctt cctggctctc ggcgatagcg atgcgttgga acacctgctg
     3121 ttcgaaggag ctgtcgtcga gctcgaggaa gccgtcctca tcgcggtagc cctcg
//
LOCUS       DAOPHV010000293         3165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_42493_length_3165_cov_3.742444, whole genome shotgun sequence.
ACCESSION   DAOPHV010000293 DAOPHV010000000
VERSION     DAOPHV010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42493_length_3165_cov_3.742444"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PLQ29_08265"
     CDS             <1..463
                     /locus_tag="PLQ29_08265"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701079.1"
                     /note="catalyzes the formation of fructose
                     1,6-bisphosphate from fructose 6-phosphate and
                     diphosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HPG86679.1"
                     /translation="KRLGALKAAGSYSGSFSALAHFFGYEGRCAFPSNFDADYCYGLG
                     YSAFVLAASGLTGYLSSIRDLSSPASEWIAGGVPLTMMMNLERRHGAEKPVIRKALVE
                     LDGAPFRAFAAARADWAVETRYRFPGAIQYYGPAEVCDRPTETLLLERPGK"
     gene            991..>3165
                     /locus_tag="PLQ29_08270"
     CDS             991..>3165
                     /locus_tag="PLQ29_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG86680.1"
                     /translation="MFRFIRRFFRKAAPPPPPGSSPQELWRSDFSSSPGRFAEVDEER
                     YALRLAEGTLRLDLRRGSLFAWADCGDYRYGDASIEAELRFGGPGPKRAAGLLLRKLD
                     DSSFLYTLVSSDGEARLDLVFNGEPRALVPWVACPWAAGADEMVLSVVLRGPRVVVLV
                     NGRFALEAEDDSLDLGGVAFGAQSYDGPATIELRSVRVDSRPLEAEADYLRFARVVAA
                     APDQRRRLAEGLFSLGYYVPALVQLRKIADGSGATAADRFLEAECLLRLELYDEAAKA
                     VDACLALEPGMDNALEERFNLLYLRGDYVGLRDALASDLERVGRSPRLANLLGHAHYN
                     LGAWAEAAEAYGAAAAGDPDMPIYSRNGAAALEKTGDLVAAAARWLAAARGFYDQGAW
                     DDAEDCSRRLRGLGYDAASLDSLDGLVAYGRGDEKTAAAILGKLWKRGKADAPAAYVY
                     GLIAAGSGERGGAIKAFRRATELEPGRAIYRYRLAESLFMAGEPFGSELAAAIEAAPD
                     DGWTLNLAGQAALTAGDAERAITLFARAKERLPDQSAPAVNLSEAYTAVGRHEEAVSV
                     LGDWPASSAQAANRLGNAYAASGRLDAAAAAYGEAVALAGPTGDPDLPEYRVNLAAAL
                     MELGRWPDAEDALRRSLEERDDARAMYLMGDIAAEYGDSSRAELAYRAAVDRDGGDSA
                     ARKRLAAHYLSRRRYDKAEAEARAIEAIDPAAAAGLLAAIREA"
BASE COUNT          483 a         1091 c         1083 g          508 t
ORIGIN      
        1 gaagcggctc ggcgcgctca aggccgccgg ttcctattcg ggttcgttca gcgccctcgc
       61 ccacttcttc ggctacgaag gccgctgcgc cttcccgtct aacttcgacg ccgactattg
      121 ctacggcctc ggttactcgg ccttcgtgct cgccgccagc ggtctgaccg gctacctgtc
      181 gtcgatacgc gacctatcga gcccggcctc agaatggata gccggcggtg taccgctgac
      241 gatgatgatg aacctcgagc gccgccacgg cgccgagaaa ccggtcatca ggaaggccct
      301 cgtcgagctg gacggcgcgc cgttcagggc cttcgcggcc gccagggccg actgggccgt
      361 cgagacccgc taccgcttcc cgggcgccat ccagtactat ggccccgccg aggtctgcga
      421 ccgcccgacc gagaccctgc tgctcgagcg cccggggaag tagctggact cgcgctaaag
      481 aagagagctt gtacgacgaa aagcggaaca tgatgatcag gatgtatcag gatgaccatg
      541 ataagggctt ttttttgggg ggggataggc cagacatcta gaagctcatc ttggtcatcc
      601 tacgccatca tggtcatcat gttctgattt cttggcgttc tttgtgcctt ggtggctcag
      661 tggcttggtg tgaggatagg aggaatgcct cacaccaagg cgcggaagcg ccaagaaaga
      721 gggccgatac aaagaagacg cggacatgat ggacacggtt tctcaagatg gacgggatgg
      781 gagctaaggc ttaaggcgct tatcccgtcc atcgggccct tttcctgtca tcctgttcat
      841 agcgtcctat tctgcccatt caagtctctc ggaacccctt ctcggtcgcg ggctcgtctt
      901 tctcagccat gggagacgcg tcgcccgacg cgtctacccg acgcgccgtg agcttgcctc
      961 gaggggcggc gccgtgtact atcgtggccg atgttccgtt tcatacgccg attcttcagg
     1021 aaagccgccc ctccgccacc gcctggctcc tcgccccagg agctctggcg ctcggatttc
     1081 agctcgtcgc ccgggcgctt cgcggaggtc gacgaggaac gttacgccct gcgtctcgcc
     1141 gagggaacgc tacgcctcga cctaaggcgc ggcagcctct tcgcgtgggc cgactgcggg
     1201 gattaccgct acggcgatgc gtcgatcgag gcggagctac gcttcggcgg cccgggcccc
     1261 aagcgggcgg ccggcctcct cctacgcaag ctcgacgact cgtccttcct gtacacgctc
     1321 gtatcgagcg atggcgaggc acggctcgac ctggtgttca acggcgagcc gcgggccctc
     1381 gtaccctggg tagcctgccc ctgggccgcc ggcgcggacg agatggtgct cagcgtcgta
     1441 cttcgcggtc cgcgcgtcgt ggtcctcgtc aacggccgtt tcgcgctcga ggcggaggac
     1501 gactcgctcg acctgggagg cgtggccttc ggtgcgcaga gctacgacgg accggcgacg
     1561 atcgagctac gctccgtgcg cgtcgattcg cgcccgctcg aggcggaagc cgactacctt
     1621 cgcttcgcgc gggtggtggc cgccgcgccc gaccagaggc ggcgcctggc ggagggccta
     1681 ttctccctcg gctactacgt gccggccctc gtgcagctgc gcaagatagc cgacggctcg
     1741 ggggctacgg ctgccgacag gttcctggag gcggaatgcc tcttgcgcct cgagctgtac
     1801 gacgaggcgg ccaaggccgt cgacgcgtgc ctggcgctcg aaccgggcat ggataacgcg
     1861 ctcgaggaac gcttcaacct gctctacctg cgcggcgact acgttggcct tcgcgacgcg
     1921 ctggcctccg atctggagcg agtcgggcgt agcccccggc tcgcgaacct gctcggccac
     1981 gcccactaca acctcggcgc ctgggccgag gcggccgagg cctacggtgc cgcggccgcc
     2041 ggggaccccg acatgccgat ctactcgcgc aacggcgcgg cggcgctcga gaagacgggc
     2101 gacctcgtgg ccgccgccgc ccgctggctc gccgccgccc gcggcttcta cgaccaggga
     2161 gcctgggacg acgccgagga ctgttcgagg cggctacgcg gcctcggcta tgacgccgcg
     2221 agcctggact cgctcgacgg cctcgtagcc tatggccggg gtgacgagaa gaccgccgcc
     2281 gctatcctgg gcaagctgtg gaaacgcggc aaggctgacg cccccgccgc ctacgtctac
     2341 ggactgatag ccgccggctc gggagaacgc ggcggcgcga tcaaggcgtt caggcgcgcc
     2401 acggagctcg agccggggcg agccatctat cggtataggc tggccgagtc gctgttcatg
     2461 gcgggcgaac ccttcggttc cgagctggcc gcggcgatcg aggccgcgcc ggacgacggc
     2521 tggacgctca acctggccgg gcaggccgcc ctgacggccg gcgacgccga acgggcgata
     2581 accctgttcg cccgggccaa ggagcgtttg ccggaccaga gcgcgccggc cgtcaaccta
     2641 tccgaggcct acaccgccgt cggacgccac gaggaggccg tctccgtcct gggcgactgg
     2701 ccggcctcgt cggcccaggc ggcaaaccgc ctcggtaacg catacgcggc cagcggacgg
     2761 ctcgatgcgg cggccgcggc ctatggcgag gccgtggccc tggccggccc cacaggcgac
     2821 cccgacctgc ccgagtatcg cgtgaacctg gccgccgccc tgatggagct gggacggtgg
     2881 cccgacgccg aggacgccct acgccgctcg ctcgaggagc gcgacgacgc ccgtgcgatg
     2941 tacctgatgg gcgacatagc ggccgagtac ggcgactcga gcagggcgga gctggcctac
     3001 cgtgcggccg tcgaccgcga cggcggggat agtgcggccc ggaagcgcct ggcggcccat
     3061 tacctgtcgc ggcggcgcta cgacaaggcc gaggccgagg ctagggccat cgaggccatc
     3121 gacccggcgg ccgcggcggg tctgctcgcg gctatccgcg aggca
//
LOCUS       DAOPHV010000294         3152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_42719_length_3152_cov_4.403294, whole genome shotgun sequence.
ACCESSION   DAOPHV010000294 DAOPHV010000000
VERSION     DAOPHV010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42719_length_3152_cov_4.403294"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..981
                     /locus_tag="PLQ29_08275"
     CDS             31..981
                     /locus_tag="PLQ29_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HPG86681.1"
                     /translation="MAGRDSIDRYGAWLLAARRRSPLTLEAYVREARSLASWLGERGL
                     AVETALVTDILEYLAYRRSSGLSARTMARIMSGIRGFFKYLRLEGLRSDDPTELLESP
                     RQERSLPDVIRADDVNLMLGSIDVATPGGLRDRALFELIYSCGLRISEAASLTFDSLY
                     LKERFIRVVGKRRKERILPFGDEARSWLTRYLEEGRPALEKGRRSDRVFLNRSGAGIS
                     RKGVWKRFSELRDASGVDGKVHSLRHSFATHLLAGGADLRTVQELLGHADISTTQIYT
                     HVDAEDLAVAHAAYFPRSGDRAQGASAEARDGVSDTGRAS"
     gene            981..1709
                     /locus_tag="PLQ29_08280"
     CDS             981..1709
                     /locus_tag="PLQ29_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG86682.1"
                     /translation="MRRTIILALAAVATLSSCGGDDATFIERMFDLESRSSKGAPLST
                     IEELKEGIRRYGDEVEKTAAAMEKVGTYWRMLAVRYLDKGLYGDAYDAATKALRHYPD
                     SSGIYYVAGMSAAFLSKTAAAQPGGGAASRAEWLAAAVGAYRRSIEIEPRNTKALYGL
                     AVLYTFELEDHESAIGPLEALLGIETKNVDAMFVYARALYGSGRLQEASDVYDRIIST
                     TRVDEKRKQAADNKKRVLDELYGG"
     gene            1690..2721
                     /gene="dprA"
                     /locus_tag="PLQ29_08285"
     CDS             1690..2721
                     /gene="dprA"
                     /locus_tag="PLQ29_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPG86683.1"
                     /translation="MSSMAAELASLRDMIVARMPFLSARQRIKLVMRVGGAPLCDLCL
                     EDVERAVGHRLDGARWDPDAWEREAERDLRFLSAVGGSSYGYFDAGYPAILRETARPP
                     FMLYVRGRLPDGRRPSVAIVGTRYPTGRGLDAALALAMEAAAEGVQVVSGLARGIDSA
                     AHRGAIAVGGRTYAVLGRGIDAVYPASNKELAARMVEAGGGIASEYPPGTPPSRWTFP
                     ERNRILAGLCRSTVVVEAPGGSGALISAAFALEEGRDVYVAAACSGGPRSAGSDALAA
                     DGAVTLSSFGDIIEDWRCGVGRHGAFEPGGFDARSRRSGNRRRRAPLRRPSRTRLTSP
                     AYGRTSRRT"
     gene            2724..>3152
                     /locus_tag="PLQ29_08290"
     CDS             2724..>3152
                     /locus_tag="PLQ29_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HPG86684.1"
                     /translation="MASDESAGMDSMNGAKEAKAGAKTVRKATKAPAKAKKTPEKPTL
                     VIVESPAKAKTIEKYLGSSYMVLASMGHLIDLPKSRLGVDVESGFAPEYLTIRGKAKL
                     LKELQKAAKKSKAVLLASDNDREGEAISFHIRAALAQKNEG"
BASE COUNT          530 a         1015 c         1110 g          497 t
ORIGIN      
        1 ccggcctcgg agcggagcgg ggtctagacg gtggcgggcc gggattcgat agaccgctac
       61 ggcgcctggc tcctcgccgc caggaggcgc tcgccgctga cgctcgaggc ctacgtccgc
      121 gaggcgcgct cgctggcctc gtggctgggc gagcgcggcc tggccgtcga gacggcgctc
      181 gtgacggata tactcgaata cctcgcgtac cgccgctcat ccggcctctc ggccaggacg
      241 atggccagga tcatgtcggg catacgcggc ttcttcaagt acctgcgcct cgaggggctg
      301 cgctccgacg acccgaccga gctcctggag agccccaggc aggaacgcag tctgccggac
      361 gtcatccgcg ccgatgacgt caatctgatg ctcggctcga tagacgtcgc gactccgggc
      421 ggtctgcgcg accgcgcgct gttcgagctg atctactcct gcggcctgcg catctcggag
      481 gcagcgtcgc tgaccttcga ttccctgtac ttgaaggaac gcttcatccg cgtcgtcggc
      541 aagcggcgca aggagcgtat cctgcccttc ggggacgagg cccgttcgtg gctgacccgc
      601 tacctggagg agggccggcc ggcgctcgag aagggcaggc ggagcgaccg cgtcttcctc
      661 aaccgatccg gcgccggtat ctcgaggaag ggcgtctgga agcgcttctc cgagctgcgc
      721 gacgcgagcg gcgtcgacgg caaggtgcac tcgctcaggc attccttcgc cacgcacctc
      781 ctagccggcg gtgccgacct acgaaccgtg caggagctac tcgggcacgc ggatatctcg
      841 acgactcaga tctatactca cgtcgacgcg gaggacctcg cggtcgccca cgcggcgtat
      901 ttccctcgat ccggcgaccg cgcccaaggg gcaagcgccg aggctcggga tggcgtctcc
      961 gatacgggga gggcaagctg atgaggcgaa cgataatcct ggccctggcg gcggtagcga
     1021 cgctgtcatc gtgcggcggc gacgatgcga cttttatcga gcgcatgttc gacctggagt
     1081 cgaggtcatc caagggcgct cctctgtcga ccatcgagga gctgaaggaa ggcatccgac
     1141 ggtacggcga cgaggtcgag aagaccgccg ccgcgatgga gaaggtcggt acctactggc
     1201 gcatgctcgc cgtccgttat cttgacaagg gcctgtacgg cgacgcgtac gatgctgcga
     1261 cgaaggcgct caggcactat ccggattcat ccggcatcta ctatgtagct ggcatgtcgg
     1321 cggccttcct ctccaagacc gcggcggctc agccgggcgg cggggcagcc tcgcgcgccg
     1381 agtggctcgc ggccgccgtc ggcgcgtacc ggcgctcgat cgagatagag cctcgcaata
     1441 ccaaggcgtt atacggcctg gcggtgctgt acaccttcga gctcgaagac cacgagtccg
     1501 ccatcgggcc gctcgaggcc ctgctcggca tcgagacgaa gaatgtcgac gcgatgttcg
     1561 tctacgcgcg cgccctgtac ggctcgggcc ggctccagga ggcctccgat gtgtacgacc
     1621 gcatcatctc gacgaccagg gtagacgaga agaggaagca ggccgccgac aacaagaaac
     1681 gcgttctgga tgagctctat ggcggctgag ctggcttcgc tacgggacat gatcgtggca
     1741 aggatgccgt tcctgtcggc taggcagcgc attaagctcg tcatgcgcgt cgggggcgct
     1801 cccctatgcg acctgtgcct ggaggacgtc gagcgggccg taggccatag gctcgacgga
     1861 gcccgttggg acccggacgc ctgggagcgc gaggccgagc gggatctgcg cttcctctca
     1921 gccgtagggg gttcgtcgta cggctacttc gatgccggct atccggcgat cctacgggag
     1981 acggccaggc ccccgttcat gctatacgtt cgcggtcgcc tgccggacgg gcgccgaccg
     2041 tcggtcgcca tagtcggtac gcgctatccg accggccgcg gcctcgacgc ggccctggcg
     2101 ctggcgatgg aagccgccgc cgagggcgtc caggtggtat cgggcctggc ccggggcatc
     2161 gactcggcgg cgcatcgcgg ggctatcgcg gtcggcgggc ggacctacgc cgtgcttggc
     2221 cgcggcatag acgcggtata tcccgcgtcg aacaaggagc tggccgcgag gatggtcgag
     2281 gccgggggcg ggatcgcgtc agaatatccg cccggcacgc cgccttcgcg ctggacattc
     2341 ccggaacgga accggatact ggccggatta tgccggtcga ccgtggtggt cgaggcgccc
     2401 ggcggttcgg gagcgctgat cagcgccgcc ttcgccctcg aggaaggccg cgacgtctac
     2461 gtggcggccg cctgttcggg cgggccgagg tcggccggtt ccgacgcctt ggccgccgac
     2521 ggggcggtga cgctatcgtc gttcggcgat atcatcgagg actggcgttg cggcgtcggg
     2581 cgccatggcg cgttcgagcc cggcggcttc gatgcgcgtt cgaggcgatc cggcaatcgc
     2641 cgacgcaggg cgccgctacg gcgaccgagc aggactaggc tgacgagccc ggcgtacggg
     2701 cgaacgagta ggagaacgta acgatggcga gcgatgagag cgctgggatg gacagcatga
     2761 acggcgcgaa ggaagccaag gccggagcta agacggtccg caaggccacg aaggccccgg
     2821 ccaaggccaa gaagacgccc gaaaagccga ctctggtcat cgtcgagtcg cccgccaagg
     2881 ccaagacgat cgagaagtac ctcggttcgt cctatatggt gctcgcgtcg atggggcacc
     2941 tgattgacct gcccaagtct cgcctcggcg tcgatgtgga gagcggtttc gcgcccgagt
     3001 acctgacgat acgaggcaag gcgaagctcc taaaggaact ccagaaggcg gccaagaaat
     3061 cgaaggcggt gctgctcgcc tccgataacg atcgcgaggg agaggccatc tccttccata
     3121 tccgagccgc cctcgcccag aagaacgagg gc
//
LOCUS       DAOPHV010000295         3150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_42756_length_3150_cov_3.540872, whole genome shotgun sequence.
ACCESSION   DAOPHV010000295 DAOPHV010000000
VERSION     DAOPHV010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42756_length_3150_cov_3.540872"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLQ29_08295"
     CDS             <1..282
                     /locus_tag="PLQ29_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HPG86685.1"
                     /translation="LYGKLMQSQPASGQNAGQMKFMMYGMPIMFFFILYDVPSGLLVY
                     WISSNVLTAVQQLVINKVIHKKRLATEAAAPQAKIVPIKSGKGGKKGRK"
     gene            315..1055
                     /gene="jag"
                     /locus_tag="PLQ29_08300"
     CDS             315..1055
                     /gene="jag"
                     /locus_tag="PLQ29_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HPG86686.1"
                     /translation="MEYEFEGRTEEEAIEIAARELGIARDEFDVEIMESQRGGFLKKG
                     KTRIRVQVRGAPAAPAPRKRPAERPDARNQASVRGDADAAPRTVANVEVDGAFERKLL
                     DFVQTTVEKMGYTCKASILFREELKLGIRLDSDSAAILIGKKGKNLDALQLLANVYAG
                     RLGREDLRVILDSENYRIRREEAIVKMAYEVAERVRSTRNSVLLEPMNPFERRIVHTT
                     LNDIVDIETKSEGDGLYKQVRVMFRGRR"
     gene            1301..1879
                     /locus_tag="PLQ29_08305"
     CDS             1301..1879
                     /locus_tag="PLQ29_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86687.1"
                     /translation="MIKRCMVIALTVAAVVCSVAAADEVGGSVYDRWPSSLGYAVNSE
                     GGAGLSWQRWFGLTGVAVTAGGLYNEDGVDSPTLYGYDYAALDYNAQLRISRRLHTAE
                     YSSWLSSNLQAVGLIAHRGYLPYEYVGYDETTYESLYARLPYVGEYMLGAGVAIEMTY
                     YGHFSQTFDFMYVAKWPLELAIAFGTSFRYRY"
     gene            1949..2458
                     /locus_tag="PLQ29_08310"
     CDS             1949..2458
                     /locus_tag="PLQ29_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86688.1"
                     /translation="MRQKRLIILVLIAAVASAGLWAQGMEDDTVFSRFPNALGAYVCY
                     PITGGLTYQHWFGRAGLQATIGGIAQTSGSFDYNVQAAFQYMLYGEDFADWFSGGLYT
                     NVLLGHRGSGTTGSDGPSYDPVEYLGLGVGIEMVFLRHLSTSVEFMYVGSYPLALEFG
                     FGGSLKYRF"
     gene            2907..>3150
                     /locus_tag="PLQ29_08315"
     CDS             2907..>3150
                     /locus_tag="PLQ29_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HPG86689.1"
                     /translation="MNTITIDAPVSDDAELVILESIYSSQKRSRALTQRDLAEAAGLS
                     LGMTNALVKRFSDKGWVLLKRLNSRNIQYALTPDGVA"
BASE COUNT          608 a          950 c          962 g          630 t
ORIGIN      
        1 ctgtacggca agctgatgca gtcccagccg gcatccgggc agaacgccgg gcagatgaag
       61 ttcatgatgt acggcatgcc gatcatgttc ttcttcatcc tgtacgacgt gccctccggc
      121 ctgctcgtgt actggatctc gtcgaacgtc ctgaccgcgg tccagcagct ggttatcaac
      181 aaggttatcc acaagaagcg cctcgcgacc gaggcggcgg ctccccaggc caagatcgtt
      241 cccataaaga gcggaaaggg cggcaagaag gggcgcaagt aactacgaaa acctctcttt
      301 ataggagtcc taatatggaa tacgagttcg aaggccgtac cgaggaagag gccatcgaga
      361 tcgccgcccg cgagctgggc atcgcccgtg acgagttcga cgtggagatc atggagtctc
      421 agcgcggcgg cttcctcaag aagggcaaga cgcggattcg agtacaggtc cggggagccc
      481 cggccgctcc ggctccgcgt aagcgcccgg ctgagaggcc ggacgcgcgg aaccaggcgt
      541 cggttcgagg cgacgccgat gcggcgcctc gaacggttgc gaacgtcgag gtcgacggcg
      601 ctttcgagcg gaagttgctc gatttcgttc agacgacggt cgagaagatg ggctatacct
      661 gcaaggcgag catcctgttc cgtgaggaac tgaagctcgg catcaggctg gacagcgaca
      721 gcgccgccat cctgataggc aagaagggta agaacctcga cgcgctgcag ctgttggcca
      781 acgtatacgc cggaaggctc ggccgcgagg acctgagggt catcctggac tcggagaatt
      841 accggatccg acgcgaggaa gccatcgtca agatggccta tgaggtcgcc gaacgggtcc
      901 gttcgaccag gaactccgtg ctcctcgagc cgatgaaccc cttcgagcgg cgcatcgtcc
      961 atacgacgct caacgatatc gtcgatatcg agaccaagag cgagggcgat ggcctgtaca
     1021 agcaggtccg cgtcatgttc aggggccgcc gctaggcgcc cagccctcta gagccgagta
     1081 gtaggccgcg cttataagcg cggcctactt tttttcttaa acctctgtcc ccacggggat
     1141 atgaaacaac gcacacaata tccacatggc ataagttatc cacaatcagt tgtgtatatc
     1201 tcgttcggcc agggtaaacc tctgtcccca gtcgtcctgt ctataggaag ccgccatacg
     1261 ttgtgctatc ttccagagta ctcgtttggg aggtaggcgt atgattaagc gctgcatggt
     1321 gattgctcta acggtagcgg cggtagtctg ttcggttgcc gcggcggatg aggtcggggg
     1381 gagcgtctac gaccgttggc cgtcgtcgct cgggtacgcc gtgaactccg agggcggcgc
     1441 tggcctgtcc tggcagcgct ggttcggcct gacgggcgtg gcggttacgg ctggcgggct
     1501 ctataacgag gatggagtcg attccccgac cctgtacggg tacgattacg cggccctcga
     1561 ctataacgcg cagctcagga tatcccgcag gctccatacc gcggaatact cgtcctggct
     1621 ctcgtcgaac ctccaggccg tcggactgat cgcccatcga ggatacctgc catacgagta
     1681 cgtcgggtac gacgagacga cctacgaatc gctatacgct cggcttccgt acgtcggcga
     1741 gtatatgctc ggcgccggcg tggctatcga gatgacgtac tacggccatt tttcccagac
     1801 cttcgacttc atgtatgtcg cgaagtggcc cctggagctg gctattgcgt tcggtacgag
     1861 cttccgctat aggtattgat cacggcgctc gaggatctcg cgggcggtga ttcgacgtag
     1921 cttgcctgag tattatagga ggtgcggtat gagacagaag agactgatca tcttggtatt
     1981 aatcgccgcg gtagcgtcgg ctgggctgtg ggcgcagggt atggaggacg atacggtctt
     2041 ctctcggttc ccgaacgctc tcggcgccta cgtctgctac ccgatcactg gcgggctcac
     2101 ctaccagcac tggttcggcc gcgccggcct ccaggccacg atcggcggca tcgcccagac
     2161 gagcggatcc ttcgactaca acgtccaggc ggcgttccag tacatgctct acggcgagga
     2221 tttcgccgac tggttctcag gcggcctgta tacgaacgtg cttctgggcc atcgcggctc
     2281 ggggacgacc ggctcggacg gcccgtccta cgatcccgtc gagtacctcg gactcggcgt
     2341 cggcatcgag atggtcttcc ttaggcacct gtcgacgagc gtcgagttca tgtacgtcgg
     2401 atcgtacccc ctggccctcg aattcggctt cggcggaagc ctgaaatacc gcttctagcg
     2461 ccaagctctg tccccgatct tttgaatata agccccgcgg cgcgtctgtt gcgccgcggg
     2521 gcttttttat gaacctctgt ccccagagag tactagagag catttccagt tcgaacctct
     2581 gtccccagaa cgccaccaga acgccatggc gcaactccgt atcaaacctc tgtccccaag
     2641 taggctcata attgatcggc tcgattgcgt attgaattcc agcacgtccc gtgctaggat
     2701 tgttcgagga atgaacaagg gctgacgatt ccgggaatgc cgcgtggcgt taccgggtcg
     2761 gtccgagtag gattccgccg tcgatcggga gcgtgtttcg gccgccccgg gccgccgcgg
     2821 aacgtcaggt tccctatcgt atccgccttt ccgcaggcgg aaggtcttcc cgtacgccgg
     2881 gcatagctcc cgctaatata actcgcatga ataccattac catagacgcg ccggtctcgg
     2941 acgacgccga gctcgtcatc ctcgagagca tctactcctc gcagaaacgc tcccgcgcgc
     3001 tgacccagcg cgacctggcg gaagccgccg ggctgtcgct cgggatgacc aacgccctcg
     3061 tcaagcgctt ctccgacaag ggatgggtgc tgctcaagcg cctgaattcg cgcaacatcc
     3121 agtacgcgct gacgcccgac ggcgtggcgg
//
LOCUS       DAOPHV010000296         3148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_42790_length_3148_cov_4.106046, whole genome shotgun sequence.
ACCESSION   DAOPHV010000296 DAOPHV010000000
VERSION     DAOPHV010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42790_length_3148_cov_4.106046"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLQ29_08320"
     CDS             <1..645
                     /locus_tag="PLQ29_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPG86690.1"
                     /translation="GAAVLVAKHLIGLGHERIAYVSTTLDEVNVIRTQRLKGLEDTLA
                     AELPSGVVTVFSRDITPSEELEDLFVEHEVGYRLTESCLSDEGVTAFVGVNDMVAYGI
                     IDAIVEAGFSIPRDYSVCGFDNVFPSRLSSIGLTTVDNYIVEKGHNAFSMLAARMAKA
                     DDGAGPDGAEPGAAAGGRAPDVITRVEYPPRLIVRGSTGPARSSAMADGRRKRP"
     gene            complement(989..2092)
                     /locus_tag="PLQ29_08325"
     CDS             complement(989..2092)
                     /locus_tag="PLQ29_08325"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HPG86691.1"
                     /translation="MDTRSSSLAGRLAVVAGLGFLSCSLFVAVRYWLTGRFAQGYLLW
                     NLLLAAAPVVIAWAGLRLSYGGRRSRAAALGAFVAWLIFYPNAPYIFTDFIHVVRRAG
                     LGSVAAPWLSEYDLLWYDIVMNAAFAFVGHYLGLVSMYLMHGAARRLFGRAAGWAAMA
                     PAILLSGLGIHLGRFSRFNSWDLLIHPVQAVRVIRESIADPAALLFSTAFSLFIALTY
                     LVFYVVKRGDIGELDGWGLGPRGQARAARASIAAAGSFDMIESMPSAASRRSSTGSST
                     VQAWARTPCAASSAAFSGVRKVWRGFRYLAPRASQYAPAPSSQADSMKATSASGQSAA
                     QMTADWWLKDEKIVRLSSPRRRIVATSRSSMPG"
     gene            complement(2110..>3148)
                     /gene="thrS"
                     /locus_tag="PLQ29_08330"
     CDS             complement(2110..>3148)
                     /gene="thrS"
                     /locus_tag="PLQ29_08330"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPG86692.1"
                     /translation="IANGKLFEASGHLGFYKEGMYSPMEIDEDDYYIKPMNCPFHMMI
                     YKNGVHSYRELPMRWAELGTVYRYERSGTLHGLMRVRGFTQDDAHIICTPEQIEDEIA
                     EVLRFSLHMHKTLGFKDIKAYLATRPAGDGAVGAPEMWDKALESLRKAVDAQGLAYEM
                     DEGGGAFYGPKIDLKVKDAIGREWQLTTIQFDFNEPERFDLTYVDADGQKKRPYMVHR
                     ALLGSIERFFGVYLEHTAGAFPIWLAPEQAVVIPVGPDFADYAAEVAAALKKAGLRAS
                     AMLSDERMNAKIRDAQKQKVPYMLVVGAKERDEGVVAVRVRDGRQLPAMPASEFVGMV
                     RGLVEARSLEL"
BASE COUNT          564 a         1038 c         1067 g          479 t
ORIGIN      
        1 ggggccgcgg tgctggtcgc caagcacctg atcgggctcg gccacgagcg catcgcgtac
       61 gtatcgacga cgctcgacga ggtcaacgtg atacgcacgc agcggctcaa gggtctcgag
      121 gacaccctcg cggccgagct gccctccggc gtcgtgacgg tgttttcaag ggatataacg
      181 ccgtccgagg agctcgagga cctgtttgtc gagcatgagg tgggctaccg gctgaccgag
      241 tcgtgcctgt cggacgaggg cgtcaccgcc ttcgtcggcg tcaacgacat ggtggcctac
      301 ggcatcatcg acgcgatagt cgaggccggc ttctcgatac cgcgagacta cagcgtctgc
      361 ggcttcgaca acgtcttccc ctcgcggcta tcatcgatag gcctgaccac ggtcgacaac
      421 tacatcgtcg aaaaaggaca caacgccttc tcgatgctgg ccgcgaggat ggccaaggcc
      481 gacgacgggg ccggaccgga cggcgccgag cccggcgcag cggcgggcgg ccgcgcgccg
      541 gatgtgatca ccagggtcga gtacccgccg agactgatcg tccgcggctc caccggcccc
      601 gccaggtcgt ccgctatggc ggacggtagg aggaaacgac catgagcagg atattcgccc
      661 accggggcgt cagcgcaagg ttcccggaga acacgatgct ctcgttcgcg gccgcgctcg
      721 agaccggctg cgacggtata gaactggacg tgcagatgag ctcggacggg gagctcgtgg
      781 tgatccacga cgagacggtc gaccggacca ccgacggctg cgggcgggta cgcgacatga
      841 gcctcgccca gctgcgcgag ctcgacgcga gcgcctcgtt caagggcgcg tatggcagga
      901 accgtatccc taccctcgac gagtacctcg acctggtggc cggggcgggt atagtcacca
      961 atatcgaact taagaacggc gtctaccgct atccaggcat ggaggagcgg ctggtcgcga
     1021 cgatacggcg ccgtgggctt gagaggcgaa cgatcttctc gtccttcaac caccagtccg
     1081 cggtcatctg cgccgcactc tgtcccgagg ccgaggtagc cttcatcgag tcggcctggc
     1141 tactcggcgc cggggcgtac tgcgaggcac gaggagcaag atacctgaac ccgcgccaca
     1201 ccttcctaac gcccgagaac gcggccgagc tggccgcgca tggcgtgcgc gcccaggcct
     1261 ggacggtcga cgacccggtg gagctacgcc ggctggcagc actcggcatc gactcgatca
     1321 tatcgaacga cccggcggcc gcgatggaag ccctggccgc cctggcctga ccccggggac
     1381 cgagccccca gccgtcgagc tcgcctatgt cgcctcgctt gacaacgtag aagaccaggt
     1441 acgtcagcgc tatgaacagc gagaaggccg tggagaacag gagcgcggcc ggatcggcta
     1501 tcgactcgcg gatgacgcgg acggcctgga caggatggat caagagatcc cagctgttga
     1561 agcgcgagaa gcggcccagg tggatgccca agcccgacag gaggatggcg ggagccatcg
     1621 cggcccagcc ggcggctcgc ccgaagagcc gccgcgccgc cccgtgcatc aggtacatcg
     1681 aaacgagccc caggtagtgc ccgacgaacg cgaaggccgc gttcatcacg atgtcgtacc
     1741 agagcaggtc gtactccgac agccagggcg ccgcgaccga gccgagcccg gcccgccgga
     1801 cgacatggat gaagtcggtg aagatgtacg gcgcgttcgg gtagaagatc aaccacgcga
     1861 cgaaggctcc cagggccgcg gcccgggagc gccggccgcc gtaggatagg cggaggccgg
     1921 cccaggctat gacgaccggc gcggccgcca ggaggaggtt ccatagcagg tagccctgcg
     1981 cgaaccggcc ggtcagccag tagcggaccg ccacgaacag cgaacaggac aggaagccga
     2041 gcccggccac gacggccagc cgcccggcca ggctactcga acgcgtatcc atggcgcccc
     2101 tcgggcggcc tacagctcga gcgagcgggc ctcgacgagg ccgcgcacca tgccgacgaa
     2161 ctcagaggcc ggcatcgcgg gtagttggcg cccgtcgcga acgcgtacgg ccacgacccc
     2221 ctcgtcgcgc tccttggccc cgacgaccag catgtacggc accttctgct tctgggcgtc
     2281 gcggatcttg gcgttcatcc gctcgtccga cagcatggcg ctcgcgcgta gcccagcctt
     2341 cttgagtgcg gcggccacct cggccgcgta gtcggcgaag tccggcccga ccgggatgac
     2401 cacggcctgc tccggtgcca gccagatcgg gaaggctccg gcggtgtgct cgaggtagac
     2461 gccgaagaaa cgctcgatcg agcccaggag ggcgcggtgg accatgtagg gtcgcttctt
     2521 ctggccgtcg gcgtcgacgt aggtcaggtc gaagcgctcc ggctcgttga agtcgaactg
     2581 gatggtggtc agctgccact cgcggcctat cgcgtccttg accttgaggt cgatcttggg
     2641 accgtagaaa gccccgccgc cctcgtccat ctcgtaagcc aggccctggg cgtcgaccgc
     2701 cttgcgcagg ctctcgagcg ccttgtccca catctccggc gcgccgaccg cgccgtcgcc
     2761 ggccggcctg gtcgccaggt aggccttgat gtccttgaaa ccgagggtct tgtgcatgtg
     2821 cagcgagaag cgcagcacct cggctatctc gtcctcgatc tgttccggag tgcagatgat
     2881 atgggcgtcg tcctgggtga aaccgcgcac gcgcatcagg ccgtgcaggg tgcccgagcg
     2941 ctcgtagcgg tatacggtgc cgagttcggc ccagcgcatc ggtagttcac ggtacgaatg
     3001 gacgccgttc ttgtagatca tcatgtggaa ggggcagttc atcggcttga tgtagtagtc
     3061 atcctcgtcg atctccatcg gcgaatacat gccttccttg tagaagccga ggtggccgga
     3121 ggcctcgaac agcttcccgt tggcgatg
//
LOCUS       DAOPHV010000297         3140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_42913_length_3140_cov_5.371475, whole genome shotgun sequence.
ACCESSION   DAOPHV010000297 DAOPHV010000000
VERSION     DAOPHV010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42913_length_3140_cov_5.371475"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1695
                     /locus_tag="PLQ29_08335"
     CDS             <1..1695
                     /locus_tag="PLQ29_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86693.1"
                     /translation="LESGESATRMAEWTRAGYGFDKSQAAQGELDGRIANALGLSKTL
                     ETGAVARAAAFRFGLLSDAAERALAAIEAARGSLDGVPSDDPQLPEAVFRYPGKALAS
                     LSSADRILAVLRADIDAFIASQASRPGYVASDASVLEWTERARTLAATAAERVADSRA
                     LTARATEQKRLADSSRLEAERRVAESRTALRAANFETARERLDRARERYLASLTIEQD
                     PALRSESDRILSELAATILKTENDLVVAETRRLVTGGRSFYLQGEFDRAESTLLQARA
                     RWSTTNSTPEVEVEYWLKLVQTALSVKTGRDIPVTAPLFPEMSQLLSLAKQYYQEGAA
                     LLAKRDKTGAIRAFSQAKQKISEVKVVFPLNQEARVLELRIDQLSDPDEFGRSFARLY
                     NEAKAKIDARADLTTAYSDLQDLEAINPRYPGLRAQIERAEILLGFRQPPPDPRAIAE
                     ARSLVAAARRIVDSGQVAQFTFARAQLEKALGLDPNNAEASALKDRIATYIGGDTAIV
                     LPSAAETLYNEAVAFFTAGDYLNARVRLTRLAAVYPRGATMQKVSDLDARLTARGY"
     gene            1701..>3140
                     /locus_tag="PLQ29_08340"
     CDS             1701..>3140
                     /locus_tag="PLQ29_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86694.1"
                     /translation="METPNPSSRRAGRGLALAAACAACALLVSHPLEAAPTDFYWEAP
                     ARLSKGIGAYPQAIRTSGDVIAIWQENVPTDGGGEAWLSMASYRNDGVVRRDRFAGPF
                     RYEAIGDKAKGERGLSAPALFSADDDGNGRLLVAASAGERRVEVYASDDSGRSFRPLS
                     ALELDRPVVAPRVYSNAAGGWYLFVTRSQVFTRTPAGGGEATTYESLSIFYTRSDDGT
                     SWAPLAPFVGGDLGLDPNFLPTAASVDGTDVVVFQTLSGGDRPSYQLYSTASRDGGLS
                     WSTPRRVTGFVDPVHREAQAPDRFDNQRPHLARIGGSLWLTWERKLLSGPAQVYAARL
                     DDDGALIQDSPERVTLGQGACSEPRLYPLGEGPDAAPAITWFDDRRGANRVYTAFKSG
                     ALWTERDLSGRSRGDGTFGRVVSTGSGLYAFWQTGSGPGSSIIGMVPDTSARPPRISA
                     VDFVPGEPTRRGRAELRWNVPEDSSGILGF"
BASE COUNT          487 a         1152 c         1066 g          435 t
ORIGIN      
        1 ctcgagtcgg gcgagtcggc gaccaggatg gccgagtgga cgcgagccgg ctacggcttc
       61 gacaagtccc aggccgccca gggcgagctg gacggccgca tagcgaatgc cctcggcctg
      121 tcaaagaccc tcgagacagg tgcggtggcc cgcgcggccg ccttcagatt cggcctcctg
      181 tccgacgcgg ccgagcgcgc gctcgcggcc atcgaggcgg cgcggggctc gctcgacggc
      241 gtgccgtcgg acgaccccca gctgcccgag gccgtgttcc gctaccccgg caaggccctg
      301 gcctcgctat cgtcggccga ccgcatactc gccgtcctcc gggccgacat cgacgccttc
      361 atcgcctccc aggcctccag gccgggctac gtggccagcg acgcctccgt gctcgagtgg
      421 accgagcggg ccaggaccct ggccgcgacg gccgccgagc gcgtggccga ctcgagggcg
      481 ctgaccgcgc gcgcgaccga gcagaaacgc ctcgccgact cgagcaggct cgaggcggag
      541 cgacgcgtcg cggagtccag gaccgcgctc agggcggcca acttcgagac ggccagggag
      601 cggctcgacc gggcccgcga gcgctatctc gcgtcgctga cgatcgaaca ggacccggcg
      661 ctcaggtccg agtccgaccg gatcctatcc gagctggccg cgaccatcct gaagaccgag
      721 aacgacctgg tcgtcgcgga gacgaggcgc ctcgtgaccg gcggacgtag cttctacctg
      781 cagggagagt tcgaccgcgc cgagtcgacg ctgcttcagg cccgcgcgcg ctggagcacc
      841 accaactcga cgcccgaggt agaggtcgag tactggctca agctcgtcca gaccgcgcta
      901 tcggtcaaga cgggccgcga catacccgtc acggcgccgc tcttcccgga gatgagccag
      961 ctcctatccc tggccaaaca gtactaccag gaaggcgccg ccctgctcgc caagcgggac
     1021 aagaccggcg ccatccgcgc cttctcgcag gccaagcaga agatatccga ggtcaaggtg
     1081 gtcttcccgc tcaaccagga ggcgcgcgtg ctcgagctgc ggatcgacca gctgtccgac
     1141 cccgacgagt tcggccgtag cttcgcgagg ctgtacaacg aggccaaggc caagatcgac
     1201 gcgcgggccg atctgacgac cgcgtacagc gacctgcagg acctcgaggc gatcaacccg
     1261 cgttacccgg gactgcgcgc gcagatcgag cgggccgaga tcctgctcgg cttcaggcag
     1321 ccgccgcccg atccgagggc tatagccgag gcgcgatcgc tggtcgccgc ggcccggcgt
     1381 atcgtcgatt ccgggcaggt cgcccagttc accttcgccc gagcccagct tgagaaggcg
     1441 ctcggcctcg atccgaacaa cgccgaggcc agcgccctca aagaccgcat cgccacctat
     1501 atcggcggcg acaccgcgat cgtgctaccg agcgccgccg agacgctgta caacgaggcg
     1561 gtcgccttct tcaccgccgg cgactacctg aacgctcgcg tcaggctgac gaggctcgcg
     1621 gccgtctatc cgagaggcgc gacgatgcag aaggtatccg acctcgacgc gcgccttacg
     1681 gcgaggggct actgacgacc atggaaacgc cgaacccgag ctcccgccgc gcgggacgcg
     1741 gactggccct ggccgcggcc tgcgccgcct gcgcgctcct tgtctcgcat ccgctcgagg
     1801 ccgccccgac cgacttctat tgggaagcgc ccgcgcgcct gtccaagggc atcggggctt
     1861 atccccaagc catcaggacc tccggcgacg tcatagcgat ctggcaggag aacgtgccga
     1921 ccgacggcgg tggcgaagcc tggctgtcga tggcctccta taggaacgac ggagtcgtca
     1981 ggcgcgatcg cttcgccggc ccgttccgct acgaggccat aggcgacaag gccaagggcg
     2041 agcgcggcct gagcgctccg gccctgttct cggccgacga cgacggcaac ggtcgactgc
     2101 tcgtcgcggc ttccgcgggg gagcgtcgcg tcgaggtcta cgcctcggac gactcggggc
     2161 gatccttccg cccgctgtcg gccctcgagc tggaccggcc ggtggtagcg ccgcgcgtgt
     2221 attcgaacgc ggccggcggc tggtacctgt tcgtcacgag aagccaggtc ttcacgagga
     2281 ctcctgccgg agggggcgag gcgacgacct acgagtcgct atcgatcttc tatacgcgct
     2341 ccgacgacgg cacgtcgtgg gcgcctctgg cccccttcgt cggcggcgac ttgggtctcg
     2401 acccgaactt cctcccgacg gccgcctccg tcgacgggac ggacgtggtc gtgttccaga
     2461 cgctgtcggg cggcgacagg ccgagctacc agctgtactc gaccgcctcg cgggacggcg
     2521 gcctgtcctg gtctacgccg cgccgcgtca ccggcttcgt cgacccggtg caccgggagg
     2581 cccaggcccc cgaccgcttc gacaaccagc ggccgcacct ggccaggata ggcggctcgc
     2641 tctggctgac ctgggaacgc aagctgctgt ccggtccggc ccaggtctac gccgcccgcc
     2701 tcgacgacga cggcgcgctt atccaagaca gcccggagcg cgtgacgctc ggccagggcg
     2761 cctgctcgga gccgcggctg tacccgctcg gcgagggacc ggacgcggcg ccggccataa
     2821 cctggttcga cgaccgccgc ggcgccaacc gcgtctatac cgccttcaaa tccggcgcgc
     2881 tctggaccga gcgcgacctt tcgggccggt cccgcgggga cggcaccttc ggcagggtcg
     2941 tctcgaccgg gagcggcctg tacgcgttct ggcagaccgg ctccggcccg ggctcgtcga
     3001 tcatcggcat ggtaccggac acgagcgccc ggccgcccag gatctcggcg gtcgacttcg
     3061 taccgggcga gccgaccagg cgcgggcgcg ccgagctgcg ttggaacgtt cccgaggact
     3121 catcgggcat actcgggttc
//
LOCUS       DAOPHV010000298         3137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_42984_length_3137_cov_3.550616, whole genome shotgun sequence.
ACCESSION   DAOPHV010000298 DAOPHV010000000
VERSION     DAOPHV010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42984_length_3137_cov_3.550616"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PLQ29_08345"
     CDS             <1..532
                     /locus_tag="PLQ29_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG86695.1"
                     /translation="WRRTIGLVLQDVYLFPGSILENVRVYDDGLSVEDAEAALRTVHA
                     ADFVSRLPGGIGAELHERGSNLSMGEKQLLSFARAVAFGAELIIMDEATASVDVATER
                     KIQRSLDVLLKGRTALIVAHRLSSILKADKILFFKDGRIIAQGRHDELIESLPEYREL
                     VRLQFPDLIAETETIA"
     gene            529..2322
                     /locus_tag="PLQ29_08350"
     CDS             529..2322
                     /locus_tag="PLQ29_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG86696.1"
                     /translation="MSEPSGGADAPRVDHIRWIGRLWKEKAWLIVVLFALTLLSSAVA
                     VTYPYLCKRLLDTIQRLLESGADDAMREVDRLLLVFAAVGVGGLVASLFPGIRGVTNS
                     IFEHAIRSKYWRKVLAKDYSFFSRFSSGDIVTRLTDDLYDFPKLSWFLCSGIFRAVES
                     ISKVAFCLGAMFLLDWRLTLLSLIPLPVMIALFYVTQDRIYDTFQKNQQAISAINSRL
                     EMSFSGVRIIKAYACEDKYKRFFSDVLKTRWNTEMAVARLEVVLHMIYQYIDYVAQVG
                     IVFAGGLMAVRGSISVGTFYAFYTYLSMLIYPILDIPQLFVSGKRAFVNIDRLEELTA
                     HPEPYRPACPAPVGDVATIGLERFTFGYGDRPGDALSDITMTLRRGERVGVIGPVGSG
                     KSTLVKALLGLLPASSGRVLVNGVDLREADIVAYRAMIGYVPQEALLFSGTLRDNVDL
                     GSDAPSDELFRVSVEAAQLTEELMSFSEGERTVVGQRGVSLSGGQKQRMAIARAVARE
                     PQFLLFDDITASLDASNEERLMRRLDQLDPDLGYLIVSHRLSTLQYVDRVLYLEGGKA
                     LAFGTHEELMDLPWYRAFITEHMGAVASETV"
     gene            complement(2349..>3137)
                     /locus_tag="PLQ29_08355"
     CDS             complement(2349..>3137)
                     /locus_tag="PLQ29_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86697.1"
                     /translation="DPSFDKYRIGLAKVERLAGTDTFSVRSSQAIAGVRGTDFGLDVV
                     ASAGGAATTRTNAYCFVGAVEVTAFIRSDAHAAESLEAIPRSYVVVAGEMLSVESRSG
                     KAAATKTALDETIRSFWVENDYVSEPEARLGGKAPTEGVAVVEPAPSTDQDEATARES
                     YERGYLQGFDEARVQFDRAFDPVAAGFLSQEEGAAIRRAAKLQSGGVIAGGLILAGGA
                     AIAVNGIMLIDAGDLAAGADALRTGAIISAAAIPFLALSLFSTP"
BASE COUNT          537 a         1042 c          990 g          568 t
ORIGIN      
        1 ctggaggcgc acgataggcc tcgtcctcca ggacgtctac ctattcccgg gtagcatcct
       61 cgagaacgtc agagtctacg acgacggcct gtccgtcgag gatgccgagg cggcgctccg
      121 cacggtgcat gccgcggact tcgtctcaag gctgcccggc ggcataggcg cggagttgca
      181 tgagcggggc tccaacctat cgatgggcga gaagcagctc ctgtccttcg ccagggccgt
      241 cgcgttcggc gccgagctga tcatcatgga cgaggcgacg gcgagcgtcg acgtggcgac
      301 cgagcgcaag atccagcgta gcctggacgt gctgctaaag ggccgcaccg cgctgatcgt
      361 ggcgcaccgc ctctcgtcga tcctcaaggc ggacaagata ctgttcttca aggatggtcg
      421 catcatcgcg cagggcaggc acgacgagct gatagagtcc ctacccgaat atcgggagct
      481 ggtacgcctc cagttccccg acctgatagc cgaaacggag acgatagcat gagcgaaccg
      541 agcggcggcg ccgacgcgcc gcgcgtagac catatacgtt ggataggccg actctggaag
      601 gagaaagcct ggctgatcgt cgtcctcttc gctctgaccc tgctgtcgag tgccgtggcc
      661 gtcacctatc cttacctctg caagcgcctc ctcgacacga tccagcgcct cctcgagtcc
      721 ggagcggatg acgccatgcg cgaggtcgac cggctgctcc tcgtgttcgc cgcggtcggg
      781 gtcggcggcc tcgtcgcctc gctcttcccg ggcatacgcg gcgtgaccaa ctccatcttc
      841 gagcacgcta tacgctccaa gtactggcgc aaggtactgg ccaaggacta ctccttcttc
      901 tccaggttct cctccggaga catcgtcacg aggctgaccg acgacctgta cgatttcccc
      961 aagctgtcat ggttcctctg ctccggcatc ttcagggcgg tcgagtcgat cagcaaggtg
     1021 gcattctgcc tgggcgccat gttcctgctc gactggcggc tgaccctgct gtcgctgata
     1081 ccgctcccgg tcatgatcgc cctgttctac gtgacgcagg ataggatcta cgataccttc
     1141 cagaagaacc agcaggcgat cagcgccatc aactcgaggc tcgagatgag cttctccggc
     1201 gtgcgcatca tcaaggccta cgcctgcgag gacaagtaca agcgcttctt ctccgatgta
     1261 ctcaagactc gttggaacac cgagatggcc gtggccaggc tcgaggtcgt gctccacatg
     1321 atctaccagt acatcgacta cgtggcgcag gtcggcatcg tgttcgcggg cggcttgatg
     1381 gccgtccggg gcagtatttc cgtaggcact ttctacgcct tctataccta cctgtcgatg
     1441 ctgatctacc ccatcctcga cataccgcag ctattcgtct cgggcaagcg cgccttcgtc
     1501 aacatcgacc gtctcgagga gctgaccgcc cacccggagc cataccgtcc cgcatgtccc
     1561 gccccggtcg gggacgtggc gacgataggg ctcgagcgat tcaccttcgg ctacggcgac
     1621 cggcccggcg acgccctgtc ggacataacg atgacgctgc gccgtggcga gcgcgtcggg
     1681 gtgatcgggc cggtaggctc gggcaagtcg acgctggtca aggccctgct cggcctgctg
     1741 ccggcttcgt ctggccgggt cctcgtcaat ggcgtcgacc tgcgcgaggc ggatatcgtc
     1801 gcctaccggg ccatgatagg ctatgtgcca caggaagccc tgctgttctc cggcaccctg
     1861 cgcgacaatg tcgacctggg ctccgacgcg ccttcggacg agctgttcag ggtctccgtc
     1921 gaggcggccc agctgaccga ggagctgatg agcttttccg aaggagagcg cacggtagtc
     1981 ggacagcgcg gcgtaagcct gtccggaggg cagaagcagc gcatggccat agcccgagcg
     2041 gtagccaggg aaccgcagtt cctcttattc gacgacatca ccgccagcct cgacgcgtcg
     2101 aacgaggaac ggctcatgcg caggctcgac cagctcgacc ccgatctggg ctacctgatc
     2161 gtgtcgcacc ggctctcgac gctgcagtac gtcgaccggg tgctatacct cgagggaggc
     2221 aaagcgctgg ccttcggtac gcacgaggag ttgatggacc ttccgtggta tcgggcgttc
     2281 atcaccgagc acatgggcgc cgtcgcctcc gagacggtct aagactcgtc ccggagatag
     2341 cggcgacgct agggagtcga gaatagcgac agcgctagga acggtatggc cgcggcgctg
     2401 attatggctc cggtcctgag cgcgtcggcg ccggccgcca ggtctcccgc gtcgatcagc
     2461 atgatgccgt tgacggctat ggccgcgccg ccggcaagga tcaggccgcc ggctatgacg
     2521 ccgcccgact gtagcttggc ggcccgacga atggccgccc cctcctcctg gctcaggaac
     2581 cccgccgcga ccgggtcgaa ggcgcggtcg aactggacgc gggcctcgtc gaagccttgc
     2641 aggtagccac gctcgtacga ctcccgggcc gtggcctcgt cctggtctgt ggatggggcc
     2701 ggctcgacta cggcgacgcc ctccgtcggg gctttcccgc cgagccgcgc ctcgggctcc
     2761 gatacgtagt cgttctcgac ccagaaactg cgtatggtct cgtccagcgc cgtcttcgta
     2821 gccgctgcct tgccggaacg actctccacc gacagcatct ccccggccac caccacatag
     2881 gagcgaggga tagcctcgag cgattcggcg gcgtgagcgt cggagcgtat gaaggccgtc
     2941 acctcgaccg ctcccacgaa gcaataggca ttcgtcctcg tggttgcggc gccgccggcg
     3001 gaggccacga cgtcaaggcc gaaatcggtg ccgcgtactc cggcgatcgc ttgggagctg
     3061 cgtaccgaga aggtatccgt accggccagc cgctcgacct tggccaggcc gatgcggtac
     3121 ttgtcgaaag atggatc
//
LOCUS       DAOPHV010000299         3132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_43082_length_3132_cov_3.596360, whole genome shotgun sequence.
ACCESSION   DAOPHV010000299 DAOPHV010000000
VERSION     DAOPHV010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43082_length_3132_cov_3.596360"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PLQ29_08360"
     CDS             <1..808
                     /locus_tag="PLQ29_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPG86698.1"
                     /translation="ENTRRYSFIFRDWESAKEAAREVMQGEFGMPSVFRLSDPEESDV
                     ALRLYGVSGTALDAAMTAFGYKPGERCLMLGTADGERGFARHVERRIKRICRRHGAMG
                     TTSYVTRAWEHGRFRDPYLREDLQDFGIMIDTLECTVTWTNLEEVHTGVRAFCHSRPG
                     TICMTHMSHFYPQGCNLYFIFIAKMDDLDEYLAYQAGILDHIQKFGATMSHHHGIGKA
                     FAPWLEGDVGPEAMAVYRALKRHFDPKGIMNPGGTLALDLPDEQRRLLRK"
     gene            863..1918
                     /locus_tag="PLQ29_08365"
     CDS             863..1918
                     /locus_tag="PLQ29_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HPG86699.1"
                     /translation="MDQTILDYAVARVVDLCGIPSPTGYTREAEAYVFAELERLGYEP
                     EYTNKRSVRVRLGGSGRPLVLAAHIDTLGAMVRSVKPSGALRVAKLGGFPENYIEGEN
                     CVVHTRGGKRFTGRFLLDKPAVHVNADVGGAKRDDLSLEIVLDEPVSKADDTKALGIG
                     SGDFVSFDPRTVVTESGYIKSRHLDDKASSGVLLALAKMIADGAASPERETWLLFTNF
                     EEVGHGGSAGLPAGVEEFLAVDMGAIGDDLGADDRSVSICAKDSGGPYDLEMTDALIA
                     LARGLELRHAVDVFPFYGSDAGAALKAGYDIRHALIGPGVQASHGYERTHREALRGAL
                     ALAGAYCCGAAAFAPAD"
     gene            1966..2670
                     /locus_tag="PLQ29_08370"
     CDS             1966..2670
                     /locus_tag="PLQ29_08370"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPG86700.1"
                     /translation="MEELPKTGLGEKGQLVATLIGLLTVGFSVSLLFASASDGSYGLV
                     AAAAVAAVAIAFAVLSASRTLDRFQRRLERSASYDQLTGAFNCMAFSVLCDHAVKEAR
                     RNGTPLSVAMFDIDDFAEVVERQGRQASDKALKLVAAGGQSGLRQSDPLCRWGGDEFV
                     ALLESCDSAGSVVAADKFRASAVGLCSAEGGMSLTLSAGVATLRPDESFYSLIGRADE
                     ALLAAKRAGRNRTVAL"
     gene            2757..>3132
                     /locus_tag="PLQ29_08375"
     CDS             2757..>3132
                     /locus_tag="PLQ29_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPG86701.1"
                     /translation="MGDPDVVQVSTLVDGESPAAVVAEAERIFRFWYGDEEWEPVARS
                     AAAIVDLFEGRFPGYRACDTDYHDIRHTMAVLLATARIADGLFIERGPYPADLTRDLL
                     VAAVCHDTGYIRTVDEDGGTGAR"
BASE COUNT          516 a         1056 c         1025 g          535 t
ORIGIN      
        1 ggagaatacg cgccgctact cgttcatctt ccgcgactgg gagagcgcca aggaggcggc
       61 gcgcgaggtc atgcagggcg agttcggcat gccgagcgtg ttcaggctgt cggaccccga
      121 ggagtcggac gtggcgctca ggctgtacgg cgtctccggc accgcgctcg acgcggctat
      181 gaccgcgttc ggctacaagc cgggcgagcg ctgcctgatg ctcggcaccg ccgacggcga
      241 gcgcggcttc gctcgtcacg tcgagcgcag gatcaagcgg atctgcaggc ggcacggcgc
      301 catgggcacc acctcctacg tcacgcgggc ctgggagcac ggccgcttcc gcgaccccta
      361 cctgcgcgag gacctgcagg acttcggtat catgatcgat accctcgagt gtaccgtcac
      421 ctggaccaac ctcgaggaag tccataccgg cgtccgcgca ttctgccact cgcggcccgg
      481 caccatctgc atgacccaca tgtcccactt ctacccgcag ggctgcaacc tgtacttcat
      541 cttcatagcc aagatggatg acctcgacga gtacctggcc taccaggcgg gcatcctgga
      601 ccatatccag aaattcgggg cgacgatgag ccatcaccat ggcatcggca aggccttcgc
      661 gccctggctc gagggcgacg tcggccccga ggccatggcc gtctaccgcg ccctcaagcg
      721 ccacttcgac cccaagggca tcatgaaccc cggcggaacg ctagcgctcg acctgccgga
      781 cgagcaacgg aggctcctga ggaagtaagc gctggccgcc ggggtcgccg cgtgtggtac
      841 aataccgcgc gaggagtaca tcatggatca aacgatactc gactacgccg tggcgcgcgt
      901 cgtcgatttg tgcggcatac cgagcccgac cggctatacc cgcgaggccg aggcctacgt
      961 cttcgccgag ctcgagcggc tcggctacga acccgagtac acgaacaagc gttcggtgcg
     1021 ggtcaggctc ggcggctccg gccgcccgct cgtgctcgcg gcccatatcg acacgctcgg
     1081 cgccatggtg cgctcggtca agccgtccgg ggcgctccgc gtcgccaaac taggcggctt
     1141 tcccgagaac tacatcgagg gcgagaactg cgtcgtgcat acgaggggcg gcaagcgctt
     1201 cacggggcgc ttcctcctgg acaagccggc ggtccacgtc aacgccgacg ttggcggcgc
     1261 caagcgcgac gacctgagcc tggagatcgt gctcgacgaa cccgtctcca aggccgacga
     1321 taccaaggcg ctcggcatcg gctcaggcga cttcgtgtcg ttcgatcctc gaaccgtcgt
     1381 caccgagtcg ggctatatca agagccgcca cctcgacgac aaggccagtt ccggagtgct
     1441 cctggccctc gccaagatga tagccgatgg cgcggcctcg cccgaacgag agacctggct
     1501 cttgtttacg aatttcgagg aggtcggcca cggcggctcg gccggcctgc ctgccggcgt
     1561 cgaggaattc ctggctgtcg acatgggcgc catcggcgac gacctcggcg ctgacgaccg
     1621 cagcgtgtcg atctgcgcca aggactcggg cgggccttac gacctcgaga tgaccgacgc
     1681 gctgatcgcc ctcgcccgcg gcctagaact gcgccacgcg gtcgatgtct tcccgttcta
     1741 cggctccgac gccggcgccg cgctcaaggc cggctacgat atcaggcacg cgctcatcgg
     1801 ccccggggta caggcgtcgc acggttacga gcggacgcac cgcgaggctc tgcgtggcgc
     1861 cctggccctg gccggcgcct actgctgcgg cgccgcggcc ttcgcgccgg ccgactaggg
     1921 cttcggtatt gcgcgattta ctgaagtggt ggacaatatc ttattatgga agaactacct
     1981 aagaccggac tgggcgagaa gggacagctc gtcgctaccc tgatcggcct cttaaccgtg
     2041 ggattctcgg tatcgttgct attcgcctcg gcatcggatg gttcttacgg gttggtagcg
     2101 gccgcggctg tggccgcggt ggccatagcg ttcgccgtcc tatcggcctc tcgtacgctc
     2161 gaccgtttcc agcgccggct cgagcgtagc gcgtcgtacg accagctgac cggcgccttc
     2221 aactgcatgg cgttctccgt cctctgcgat cacgcggtca aggaagccag gcggaacggt
     2281 acgcccctgt cggtggcgat gttcgatatc gacgatttcg cagaggtcgt cgagaggcag
     2341 ggcaggcagg cttccgacaa ggccctgaag ctggtcgccg ccggcggcca gtccgggcta
     2401 cggcaaagcg acccgctgtg ccgctggggc ggcgacgagt tcgtcgcgct gctcgagtcc
     2461 tgcgactcgg ccggctcggt cgtggccgcc gataagttca gggcctccgc cgtcgggctg
     2521 tgttccgccg aaggcggcat gtcgctcacc ctgagcgccg gggtcgccac gctcaggccc
     2581 gacgagagct tctactccct tatcggccgc gccgacgagg ccctgctggc cgcgaagcgc
     2641 gccggccgga accgtaccgt cgctctctga gccgctcgtt ttttcttcgc gcggcctgtc
     2701 tcgtcggcct cggcgtgcta tcataaaatg gatacgcaaa taaggaggat ccgtcgatgg
     2761 gcgatcccga tgtcgtgcag gtatccacgc tggtcgacgg agagagtccc gcggccgtag
     2821 tcgcggaggc cgagaggatt ttcaggttct ggtacgggga cgaggaatgg gagcccgtgg
     2881 cccgatcggc cgcggccata gtcgacctgt tcgaagggcg tttcccgggc taccgggcct
     2941 gcgatactga ctaccacgat atacgccata ccatggccgt cctgctcgcc acggcgagga
     3001 tagccgacgg cctgttcata gagcgcgggc cgtatcccgc cgacctgacg cgcgacctgc
     3061 tcgtcgcggc cgtctgccac gataccggct atatacgaac agtggatgag gatggcggta
     3121 ccggagcccg ct
//
LOCUS       DAOPHV010000300         3122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_43276_length_3122_cov_3.827519, whole genome shotgun sequence.
ACCESSION   DAOPHV010000300 DAOPHV010000000
VERSION     DAOPHV010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43276_length_3122_cov_3.827519"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="PLQ29_08380"
     CDS             <1..1100
                     /locus_tag="PLQ29_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPG86702.1"
                     /translation="MGYCPGIENYSGPLSGRARGQRPSVLLDYFPDDFLTFIDESHVT
                     LPQVGAMYAGDRSRKQNLVDYGFRLPCALDNRPLKFDEFEGLTRRVIYVSATPGVAEV
                     GKSARVVEQMIRPTGLVDPEILVRPSEGQMEDIYAEVKRRIALGERSLILTLTKRMAE
                     ELSEYLSSLSLKVRYIHSEVETIERVEILTQLRTGEYDVLVGINLLREGIDLPEVSFI
                     AILDADKIGFLRSATSLVQIIGRAARNASGVVVMYADRMSDAMHVAIDETNRRRAVQQ
                     AYNEAHGITPTTVKKAIHDILQRHKEEKEQALDAELETVKGAYNLLVPEQKAALVKRL
                     EQEMLERAKNLEFEAAAILRDEIERIKAGES"
     gene            1097..2056
                     /locus_tag="PLQ29_08385"
     CDS             1097..2056
                     /locus_tag="PLQ29_08385"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG86703.1"
                     /translation="MNICLPVRFESRATLTAEGRAAVEALARECLDSDGFDPCLHYDT
                     HLNADRDLRPWRLAWACAATCRGEPCRADAAVDDGDGRPRPARLVAAASAFAPLRSEG
                     EISACVSPLFRHQGLFKALYGGLEASLCRSGARSVLLVCAARSPASRSIAGRLDAKLD
                     HAELRMRLDPDALERVEPPADLRLVPVGEDDADDCSRLAATIFGDSYDDERAFVEAVL
                     RDEARELFMAHGADGAVGMATITRGEAGYELNGVGVLPEHRGKGYGSAIMDACLVVLR
                     NRGVRDVTLEVDAANEPAMALYRSRGFAETARVDYWRLPTRCS"
     gene            2061..2582
                     /locus_tag="PLQ29_08390"
     CDS             2061..2582
                     /locus_tag="PLQ29_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPG86704.1"
                     /translation="MDDDYRFDEEGYLESCGAEFRRARLERGLTREALSELSGVHVNT
                     IGAVERGDHDINGSTKCRLLAALGCKSVNMTDSYDEIVLHDDPGAFPRADIQEMPESL
                     VVRIIGQAMRERRESLGLTLEDVAGRAGLHLNTLWNIERGLVSPMGIHIHRVYRALGV
                     SVVTPSPETLILE"
BASE COUNT          572 a         1048 c         1002 g          500 t
ORIGIN      
        1 agatggggta ctgccccggc atcgagaact attccgggcc gctgtcgggg cgggcgcggg
       61 ggcagcgccc gtcggtgctg ctcgactact tccccgacga cttcctgacc ttcatagacg
      121 agtcgcacgt aaccctgccg caggtcggcg cgatgtacgc gggcgaccgt tcgcgcaagc
      181 agaacctcgt cgattacggc ttcaggctac cctgcgccct cgacaaccgg ccgctcaagt
      241 tcgacgagtt cgagggcctg accaggcgcg tcatctacgt gtcggcgacg cccggggtcg
      301 ccgaggtcgg caagtcggcc cgcgtcgtcg agcagatgat acgcccgacc ggcctcgtcg
      361 acccggagat actcgtacgc ccgagcgagg ggcagatgga ggacatctac gccgaggtca
      421 agcgccgcat agcgctcggc gagcgcagcc tgatcctcac gctgaccaag cgtatggccg
      481 aggagctgtc cgagtacctc tcgtccctgt cgctcaaggt gcgctacatc cacagcgagg
      541 tggagacgat agagcgcgtc gagatactca cccagctacg gaccggcgag tacgacgtgc
      601 tcgtcggcat caacctgctc agggaaggca tcgacctgcc cgaggtgtcg ttcatagcga
      661 tactcgacgc ggacaagata ggtttcctgc gctcggccac cagcctcgta cagatcatcg
      721 gccgcgccgc gcgcaacgcc tcgggcgtcg tcgtcatgta cgccgaccgg atgtcggacg
      781 ccatgcacgt ggccatcgac gagacgaacc gccgccgcgc ggtccagcag gcctacaacg
      841 aagcccatgg tatcaccccg accaccgtga aaaaggccat ccacgacatc ctccagcgcc
      901 acaaggagga gaaggaacag gcgctcgacg ccgagctcga gacggtcaag ggcgcctaca
      961 acctgctcgt acccgagcag aaggccgccc tcgtcaagcg gcttgagcag gagatgctcg
     1021 agcgggccaa gaacctcgaa ttcgaggccg ccgcgatact ccgcgacgag atcgagcgca
     1081 tcaaggccgg cgagtcgtga atatctgcct gcccgtgcgc ttcgagagca gagccacgct
     1141 gacagcggaa ggccgcgctg ccgtcgaggc gctcgcccgc gaatgcctcg atagcgacgg
     1201 cttcgacccg tgcctgcact acgataccca cctgaatgcc gaccgagacc tgcgcccttg
     1261 gcgcctggcc tgggcctgcg cggcgacgtg ccgcggcgaa ccgtgccgcg ccgacgcggc
     1321 cgtcgacgac ggggacgggc gcccgcggcc ggccaggctc gtcgccgctg cgtccgcctt
     1381 cgcgccgctg cgctccgagg gcgagatcag cgcctgcgtc tcgccgctct tcaggcatca
     1441 ggggctgttc aaggccttat acggcggcct cgaggcgtcg ctatgccgtt cgggagcccg
     1501 ttcggtgctg ctcgtctgcg ccgcccggtc accagcgtct cgctcgatag ccggccgcct
     1561 cgacgccaaa ctcgaccacg ccgagttgcg tatgcgcctg gacccggacg ctctcgagcg
     1621 ggtcgagcca ccggccgacc tgcgtctcgt accggtcggc gaggatgacg cagacgactg
     1681 ctccaggctg gcggcgacca tcttcggcga tagctacgat gacgagaggg ccttcgtcga
     1741 ggcggtcctg cgcgacgagg cccgcgagct gttcatggcg catggggcgg atggggccgt
     1801 cgggatggcc accatcaccc ggggcgaggc cgggtacgag ctcaatggcg tcggcgtcct
     1861 gcccgagcat cgcggcaagg gctacggttc cgcgatcatg gacgcctgcc tcgtcgtcct
     1921 gcgcaacaga ggcgtgcgcg acgtcaccct cgaggtggac gccgcgaacg agccggccat
     1981 ggcgctctac aggtcccgcg gattcgccga gacggccagg gtcgactact ggcgcctgcc
     2041 gacgaggtgc tcgtaacggt atggacgacg actatcgctt cgacgaggaa ggctatctcg
     2101 agtcgtgcgg cgccgagttc aggcgcgcca ggctcgagcg cggcctgacg cgcgaggcgc
     2161 tgtcggagct ttcgggcgtc cacgtcaata cgataggagc ggtcgagcgc ggcgaccacg
     2221 acatcaacgg ctcgaccaag tgccgcctcc tcgccgccct cggctgtaag agcgtcaaca
     2281 tgacggacag ctacgacgag atcgtcctgc acgacgatcc cggcgccttc ccgcgcgccg
     2341 atatccagga gatgcccgag tcgctcgtcg tgcgcatcat aggccaggcc atgcgcgagc
     2401 gccgcgagtc gctcggcctg accctcgagg atgtggcggg ccgggcgggg ctgcacctga
     2461 acacgctctg gaacatagag cgaggcctcg tcagcccgat gggcatccat atccaccgcg
     2521 tctatcgagc cctcggcgtc agcgtcgtca ccccgagccc ggagaccctg atcctcgagt
     2581 gagccgcggc gtcgccgccc ggcttttcac aatccactgt gtagtgcgct cgaagagacg
     2641 atgatagctt tctcgtaggc ccgcttggcg ggcgagaaac gttacgagga gataacgatg
     2701 cgaagagact attctatcta ccttacttgc gtgcgtagca ctcctaatcg gtcttgtttc
     2761 atgcgatcct aataatatac ccgaagagag cttcggaacg agcatcaccg aggatacgac
     2821 caccgtagct ggaacctata cgatttccgg aaacctaacc gtagatgcgg tatggactat
     2881 agagccaggg acgattatca acatcgctcc tgattgcggt atcgacgtat cgtcaacagg
     2941 cagaatccta gccgtcggta cttctgactc gactatcacg ttccgatcgg gcaatacggt
     3001 cccggcgaag ggcgactggt atggcatcga cgacgacggt aacggctctc agtacgagta
     3061 ttgcgtcatc agccacgccg acaccggcct tgacctcggc gcctcggctg cgagcgtcaa
     3121 ac
//
LOCUS       DAOPHV010000301         3117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_43366_length_3117_cov_3.218158, whole genome shotgun sequence.
ACCESSION   DAOPHV010000301 DAOPHV010000000
VERSION     DAOPHV010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43366_length_3117_cov_3.218158"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /gene="dnaA"
                     /locus_tag="PLQ29_08395"
     CDS             <1..897
                     /gene="dnaA"
                     /locus_tag="PLQ29_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPG86705.1"
                     /translation="LGKTHLMQAIGNRAWSLNPKLKIICVSAENFTNDFIHSIHEKAM
                     SSFKNRYRTVDILLIDDIHFFENKEGSQEELFHTFNALYDGNKQMVFTCDRPASELKN
                     LSDRLRSRFERGLNVDLQPPNYETRYAILKKKVETNNLNIPPEVFDVIARNISTNVRD
                     LEAALVKLNAYADLVGKEITLEVAQQQLKDVFSQTRHGNVTIDHIIRTVSEAYKVSAN
                     DLKGKKRTRSIVVPRQIAMYIAREITEYSTTELGLEFGGRDHTTVMHSCQKIDEQLKL
                     DPMLDAKIQKLIQAVRQMSVKS"
     gene            1199..2302
                     /gene="dnaN"
                     /locus_tag="PLQ29_08400"
     CDS             1199..2302
                     /gene="dnaN"
                     /locus_tag="PLQ29_08400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPG86706.1"
                     /translation="MKFVCEKNALQREIAFAQEIISSKSAMSVVSNVYLEAREGSLLI
                     RATDIKIGFETRMPVEVQEEGSLTVFCDKLTGILASIPEGTIEVDQKDAKVVIKPSFK
                     KAQFSLKTIGSEQFPQLPDIDDDKYFSIPIADFKDMIGQTIFAVSDDETRYFMNGVYL
                     ERAQDGLVMVATDGRRLAYIKRGIVEGVPEFKGVIIPPKLLSVVLHRAGNEGQIDLAV
                     SDKNLFVKFGQYRLSTVLIEGQFPNYQKVVPESQKYTVSVKRQDVIDALKRVSIFVEQ
                     KSRRTFFALSADKLVISSEETEIGTAREEILCAYEGPEAVIAMNYKYIEDPLRAISTE
                     DISMRFTETSRAVTVAPDPESDYFHILMPMQVD"
     gene            2321..>3117
                     /locus_tag="PLQ29_08405"
     CDS             2321..>3117
                     /locus_tag="PLQ29_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HPG86707.1"
                     /translation="MPFSRIESVSFRNLADGAIQTEAPSVFVVGENGQGKSNFLDAVY
                     TLCYGSSFRGGQDADACRNGERAWSLRGVSPDGSNCVIAWEAGTKAIKEDGKPVRDRK
                     RLVERNPAIVFCHEDMEFSRGEPERRRFFFDQTASLVSVGYIDLLRAYRRTLKARNQA
                     LKDRSLDVLEVLDLQLASYGLGLRDARAAIAREFDGRFAPRFESVSRLGYRVGVSYRP
                     SWRLDEGPDEVVARLAAARDRELALGTSLSGPHRDRFAFTDDSGDFS"
BASE COUNT          711 a          959 c          893 g          554 t
ORIGIN      
        1 ctcggcaaga cgcacctgat gcaggctata ggcaatcgag cctggtcgct caaccccaag
       61 ctcaagatca tctgcgtctc ggcggagaat ttcacgaacg acttcatcca ctcgatccac
      121 gagaaggcca tgtcgtcgtt caagaaccgc taccgcaccg tcgatatact cctgatcgac
      181 gatatacact tcttcgagaa caaggagggc tcccaggagg agctgttcca taccttcaac
      241 gcgctgtacg acggcaacaa gcagatggtc ttcacctgcg accggccggc ctccgagctc
      301 aagaacctgt ccgacaggct acgcagccgg ttcgagcgcg gcctcaacgt cgacctgcag
      361 ccgcctaact acgagacccg ctacgccatc ctcaagaaga aggtcgagac gaacaacctg
      421 aacatcccgc cggaggtctt cgacgtgata gccaggaaca tctcgacgaa cgtccgcgac
      481 ctcgaggcgg ccctcgtcaa gctcaacgcg tacgcagacc tggtgggaaa ggagatcacc
      541 ctcgaggtgg cccagcagca gctcaaggac gtgttcagcc agaccaggca cggcaacgtg
      601 acgatagacc atatcatccg cacggtatcg gaggcctaca aggtcagcgc caacgacctc
      661 aagggaaaga agcgcacgag gagcatcgtc gtccccaggc agatcgccat gtacatagct
      721 agggagataa ccgagtattc gacgaccgag ctcggactcg agttcggcgg tcgcgaccat
      781 acgaccgtca tgcactcatg ccagaagata gacgagcagc tcaagctcga cccgatgctc
      841 gacgccaaga tccagaagct catccaggcc gtcaggcaga tgagcgtaaa aagctaagtg
      901 gttgatggtc gggggccttc ctgtagaaaa gggaggcgtc cccgctatcc ttcatgcgcg
      961 cccgtggaca tgtgcagatc cgcgcgcgcc tatagccatg gttatcaaca gcccaagtca
     1021 cggcggggca tgaaacgaac gggtttttcc acttatacag gccccttact atatctatta
     1081 cgaatttata taaattcaag ttcaataagg atgagaaccc gaagccctca tatcctttta
     1141 ttgcgcgtca tcgcgcatcc gtggtattct tagcggaaga ctcagtacga ggttagccat
     1201 gaaattcgtc tgcgaaaaaa acgcgctgca acgggagata gccttcgccc aggagatcat
     1261 ctcctcgaag agcgccatgt cggtcgtatc gaacgtgtat ctcgaggccc gcgagggttc
     1321 gctcctgata cgggctaccg atataaagat aggcttcgag accaggatgc ccgtcgaggt
     1381 gcaggaggaa ggcagcctga ccgtcttctg cgacaagctg accggcatac tcgccagcat
     1441 ccccgagggc accatcgagg tcgatcagaa ggacgcgaag gtcgtcatca agcctagctt
     1501 caagaaggcc cagttcagcc tcaagaccat aggcagcgag cagttccctc agctccccga
     1561 tatcgacgac gacaagtact tttccatacc gatcgccgat tttaaggaca tgatagggca
     1621 gacgatcttc gccgtctccg acgacgagac caggtacttc atgaacggcg tctacctcga
     1681 gcgcgcccag gacgggctgg tcatggtagc gaccgacggc cgccgactgg cgtacatcaa
     1741 gcgcggcatc gtcgaaggcg tcccggagtt caagggcgtc atcataccgc cgaagctctt
     1801 gtcggtcgtg cttcaccgcg ctggcaacga gggacagata gacctggccg tcagcgacaa
     1861 gaacctcttc gtgaaattcg gccagtaccg cctgagcacc gtgctcatcg aggggcagtt
     1921 cccgaactac cagaaggtcg taccggaaag ccagaagtat accgtatcgg tcaagcgcca
     1981 ggacgtgatc gacgcgctca agcgcgtctc gatcttcgtc gagcagaagt ccaggcgcac
     2041 cttcttcgcc ctatcggccg acaagctggt catatcgtcg gaggagaccg agataggcac
     2101 cgcgcgcgag gaaattctct gcgcctacga gggccctgag gcggtcatag cgatgaacta
     2161 caagtatatc gaggatccgc tccgcgccat ctcgaccgag gacatctcca tgcgcttcac
     2221 cgagacctcc agggccgtca ccgtggcgcc cgatccggaa tccgattatt tccacatcct
     2281 gatgccgatg caggtagact agagcggcag acgggactcc atgccgttca gccgtatcga
     2341 gagcgtatcc tttcgcaacc tagcggacgg ggccatccag acggaggccc cgtccgtttt
     2401 cgtcgtcggc gaaaacgggc agggcaagag caacttcctc gatgccgtgt acacgctctg
     2461 ctacggctcg agtttcagag gcgggcagga cgccgacgcc tgtaggaacg gggagcgagc
     2521 ctggtcgcta cgaggggtca gccccgacgg ttcgaactgc gtcatcgcct gggaagccgg
     2581 aaccaaggct atcaaggagg acggcaagcc ggtacgagac cgtaagcgcc tcgttgagcg
     2641 caacccggcc atcgtgttct gccacgagga catggagttc tcgcgcggcg agcctgaacg
     2701 gaggcgcttc ttcttcgatc agaccgcgag cctagtctcg gtaggctaca tcgaccttct
     2761 gcgggcctat cgccgaaccc tcaaggccag gaaccaggcg ctcaaggatc ggagtctgga
     2821 cgtactcgag gtactcgacc tccagctagc cagctatggc cttggcctgc gtgacgcgcg
     2881 cgccgcgata gcccgagaat tcgatggtcg tttcgcgccg cgcttcgaga gcgtctcaag
     2941 gctcggctat cgagtcggcg tatcgtatcg tccatcctgg aggctcgatg aaggccccga
     3001 cgaggtcgta gccaggctgg ctgccgccag agatcgagag ctggccctcg gcacgagcct
     3061 atcgggtccg caccgcgacc gtttcgcctt taccgacgac tccggcgact tctccga
//
LOCUS       DAOPHV010000302         3108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_43540_length_3108_cov_3.502457, whole genome shotgun sequence.
ACCESSION   DAOPHV010000302 DAOPHV010000000
VERSION     DAOPHV010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43540_length_3108_cov_3.502457"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3108)
                     /gene="rpoB"
                     /locus_tag="PLQ29_08410"
     CDS             complement(<1..>3108)
                     /gene="rpoB"
                     /locus_tag="PLQ29_08410"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPG86708.1"
                     /translation="ELPDLIDIQLSSYERFLQREKVLSGSLPDEQGLEEVFKATFPIE
                     SPNGDMLLEYEHYQLDEEAMKYSEYECKQKGLTYAVPLKARINLVFQKTGEIRQKDIY
                     LGDIPLMTDRGTFIINGAERVVVSQIHRSPGVIFCHEKGIYSARIIPYRGSWLEFEID
                     SKKELIYAKIDRKKRILGSMFLRAVGLETREDIIAAFYKTRSIKISEARGDRDGAVNA
                     VLARSVWIEQDGERKKMYRAGEKIHLHDVDEMVALGVSELEVVDFDGEDGLHWNMILN
                     CLEREDIKLIKEDPSQDEPTKADALAAVYSTLMPGEPITVENAERDLHSLFFTSRRYD
                     LGKVGRYKLNKKFDYVVPVKDFTLTREDIIATMKYLMTVYYGENNLDDIDHLGNRRVR
                     SVGELLANVMKSAFSRMERIAKERMSLKETDTIRPQDLISIKPVVAAIKEFFGSSQLS
                     QFMDQVNPLAELTHKRRLNALGPGGLSRDRAGFEVRDVHYTHYGRMCPIETPEGPNIG
                     LIVSLATYTTVNEYGFLETPYRKVRDGKVTDDIEFLSAMDEDKYYVAQASAKIDKDGN
                     FLESQVSCRHQGDYTTRTAPELQYMDVSPKQIISVSAALIPFLEHDDANRALMGSNMQ
                     RQAVPLLFTDPPRVGTGMEKKCAYDSGVLIKAKRSGVVAWVDALRVVIKPDADPKARD
                     EYLLIKYQRTNQDTCYNQRPIVKAGEKILKGQVIADGPATKDGELALGRNILAGFVPW
                     NGYNYEDAILISERVVRDDLFTSVHIKEFQTEVRETKLGPERITRDIPNTSEKSLDNL
                     DAEGIIRIGAKVNAGDILVGKVTPKSETETTPEFKLLNSIFGEKAKDVRDTSLRVPHG
                     IEGTIIDVQRLKRSANDDLSPGVEEVVKVLIAAKRKLKEGDKMAGRHGNKGIVARVLP
                     VEDMPYLDDGTPLDICLNPLGVPSRMNIGQLMETELGWIGSTLDEWFSSPVFQSPDQE
                     MIEAKLQEAGLPASCKTPVRDGRTGEHFQNPVTVGVIYFMKLHHLVDDKMHARSTGPY
                     SLVTQQ"
BASE COUNT          492 a          926 c         1025 g          665 t
ORIGIN      
        1 ctgctgggta accagcgagt acgggccggt cgaacgagcg tgcatcttgt cgtcgaccag
       61 gtggtgcagc ttcatgaagt agatgacgcc gacggtgacc gggttctgga aatgctcgcc
      121 ggtacggccg tcgcggaccg gcgtcttgca cgacgcgggc agccccgcct cctggagctt
      181 ggcctcgatc atctcctggt cgggcgactg gaacaccggg gagctgaacc actcgtccag
      241 agtcgatccg atccacccca gttcggtctc catcagctgg ccgatattca tgcggctcgg
      301 cacgccgagc gggttcaggc agatatcgag cggggtgccg tcgtcgaggt acggcatgtc
      361 ctccacgggt agcacgcggg cgacgatgcc cttgttaccg tggcggccgg ccatcttatc
      421 gccttccttg agcttgcgct tggctgcgat cagtaccttg accacctctt cgacgccggg
      481 ggacaggtcg tcgttcgcgg agcgcttgag gcgctgcacg tcgatgatcg tgccctcgat
      541 gccgtggggc acgcggagcg aggtgtcgcg gacgtccttg gccttctcgc cgaaaataga
      601 gttgaggagc ttgaactccg gcgtcgtctc ggtctcgctc ttgggcgtga ccttgccgac
      661 caggatgtcc ccggcgttga ccttggctcc gatgcggatg ataccctcgg cgtccaggtt
      721 atccaggctc ttctcgctgg tgttcgggat gtcgcgcgtg atgcgctccg gcccgagctt
      781 ggtctcgcgg acctcggtct ggaattcctt gatatggacc gacgtgaaca ggtcgtcgcg
      841 gacgacgcgc tcggagatga ggatggcatc ctcgtagttg tagccgttcc acggcacgaa
      901 gccggccagg atattacggc ctaaggcgag ttcgccgtcc ttggtcgctg ggccgtcggc
      961 gatgacctgc cccttgagta ttttctcgcc ggccttgacg atgggccgct gattatagca
     1021 ggtatcctgg ttggtacgct ggtacttgat cagcaggtac tcgtcgcggg ccttggggtc
     1081 ggcgtccggc ttgatcacga cgcggagcgc gtcgacccag gccaccacgc cggagcgctt
     1141 ggccttgatc agcacgcccg agtcgtaggc gcacttcttc tccatgccgg tgccgacgcg
     1201 cggagggtcg gtaaagagga gcggcaccgc ctggcgctgc atgttcgagc ccataagggc
     1261 gcggttggcg tcgtcgtgct cgaggaaggg gatgagcgcc gcggagaccg atatgatctg
     1321 cttgggcgac acgtccatgt actgaagctc gggcgcggtc ctggtcgtgt agtcgccctg
     1381 gtgcctgcac gacacctggc tctcgaggaa gttgccgtcc ttgtcgatct tggcgctggc
     1441 ctgggcgacg tagtacttgt cctcgtccat cgcggacagg aactcgatgt cgtcggtcac
     1501 cttgccgtcc cgcaccttac ggtacggcgt ctcgaggaag ccatactcgt tgacggtggt
     1561 atacgtggcg agcgacacga tgaggccgat attcgggcct tccggcgtct cgatcggaca
     1621 catgcggcca tagtgcgtgt agtgcacgtc acgtacctcg aagccggctc gatcacggga
     1681 caggccgccc gggccgagcg cgttgaggcg ccgcttgtgg gtcagctccg cgagcgggtt
     1741 gacctgatcc atgaactggg acagctggct ggagccgaag aactccttga tcgcggcgac
     1801 gacgggcttg atcgagatca ggtcctgcgg ccgtatggta tcggtctcct tgagcgacat
     1861 gcgctccttg gcgatacgct ccatgcggct gaaggcgctc ttcatcacgt tggccaggag
     1921 ctcgccgacc gagcgtacgc ggcggtttcc gaggtggtcg atatcgtcga ggttattctc
     1981 gccgtagtag acggtcatca ggtacttcat cgtcgcgatg atgtcctcgc gagtcagcgt
     2041 gaagtccttg accggtacca cgtagtcgaa tttcttattg agcttgtagc gaccgacctt
     2101 gcccaggtcg tagcgacggc tcgtgaagaa cagcgagtgg aggtcgcgct cggcgttctc
     2161 caccgttatc ggctccccgg gcatcaaagt cgagtagacg gcggcgaggg cgtcggcctt
     2221 ggtcggctcg tcctggctcg ggtcttcctt gatcaacttg atgtcctcgc gttcgaggca
     2281 gttgaggatc atgttccagt gcaggccgtc ctcgccgtcg aagtcgacga cctcgagctc
     2341 ggagacgccc agggccacca tctcgtcgac gtcgtggagg tggatcttct cgccggcgcg
     2401 gtacatcttc ttgcgctcgc cgtcctgctc tatccagacg gacctggcga gcaccgcgtt
     2461 gacggcgccg tcgcggtcgc cgcgggcctc ggagatcttg atggatctcg tcttgtagaa
     2521 cgcggcgatg atgtcctcgc gcgtctcgag tccgacggcc ctgaggaaca tggagccgag
     2581 gatacgcttc ttgcggtcga tcttggcgta gatcagctcc ttcttggagt cgatctcgaa
     2641 ctcgagccac gagccgcgat acgggatgat gcgggcggaa tagatgcctt tctcatggca
     2701 gaaaatgacg cccggcgacc tgtggatctg cgacacgacg acgcgctcgg cgccgttgat
     2761 gatgaaggtg ccgcgatcgg tcatcagcgg gatatcgccg aggtagatat ccttctggcg
     2821 tatctcgccg gtcttctgga acacgaggtt gatacgggcc ttgaggggga ctgcgtaggt
     2881 gaggcccttc tgcttgcact cgtactcgga gtacttcatc gcctcctcgt cgagctggta
     2941 atgctcgtac tcgaggagca tgtctccgtt cgggctctcg atcgggaagg tcgccttgaa
     3001 aacctcctcg agcccctgct cgtcgggcaa gctgcccgaa agcaccttct cgcgctgcag
     3061 gaagcgctcg tagctggaca gctgtatgtc tataagatcg ggaagctc
//
LOCUS       DAOPHV010000303         3105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_43589_length_3105_cov_3.559016, whole genome shotgun sequence.
ACCESSION   DAOPHV010000303 DAOPHV010000000
VERSION     DAOPHV010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43589_length_3105_cov_3.559016"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..1082
                     /locus_tag="PLQ29_08415"
     CDS             45..1082
                     /locus_tag="PLQ29_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPG86709.1"
                     /translation="MSKDGKRAALAYAGLITLLAASFAAAIALGAVRLSPVEAARALL
                     SSREASSPRVASSPEAIIVGIRFARACLAAAVGASLAASGAVFQGLFRNPLADPYVIG
                     SSGGAALGAVCAIAFGSGAGALGFSGIGLAAFAGGLGATMVVYLVAGAPRLRSDASSL
                     LLAGTALGSLFSALVSIVLALKDKDLHQAWFWLLGGFSGRGLPELAASAPAMLAGFAA
                     AMAGSRSLDLLSVGDEEATSLGLRPAATRFVVGGFAALSVSAAVALSGAIGFVGLVSP
                     HIARRFVGPRHVRLIPAAALVGASLLVVADVVARTAFAPVEMPVGAMTALIGAPFFVY
                     KIARRGLDGAR"
     gene            1079..1885
                     /locus_tag="PLQ29_08420"
     CDS             1079..1885
                     /locus_tag="PLQ29_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG86710.1"
                     /translation="MSALLEARGLSAGYGERVVVAGASFSVSAGSLLVVLGPNGCGKT
                     TVLKTCLGLLPPLSGDALLGGLPVTGLRPRERARLAAWVPQLAESAWSYRSRDIVAQG
                     RFAARGPFMPLNGDDEAAVDAALDAMDARAFADRPFSSLSGGEARRVMIARALAQETP
                     LIVLDEPAAHLDPGRQMELMELLAGLARSGKAVAVSVHDVNAAKRFSDLALLVGKDGS
                     AAFGAPGDVLTPENLENAYDTHFIHGDHESYGPYVLPLSRKRAKESHDGR"
     gene            1872..2930
                     /gene="cobT"
                     /locus_tag="PLQ29_08425"
     CDS             1872..2930
                     /gene="cobT"
                     /locus_tag="PLQ29_08425"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HPG86711.1"
                     /translation="MMDDKRILSEVSALLDDLTKPKGSLGRLEDYASRLAVIQGRVPP
                     RVRRKAAFVLAGDHGVVSRGVSLYPKEVTAQMFANIMAGGAGINVLSRACGFDVFVVD
                     AGVDADLPDYPSGGADGKPRYVPMKAVRGTRDFTVEPAFTEIEFLQCLHNGQKLAELA
                     VREGYDIVALGDMGIGNTTSAAAVLAGYGFDPDLVVDRGTGIDDEALERKRALITEAV
                     AARGPYPTPESVGAAFAGPEIATAAGVILGLKGKGVACMLDGFPITAGAVIAWKIDPS
                     VTDYLFAGHQSRVKGHRPVLEALGLEPVVSLDMRLGEGTGAVIGGFIVELGARIAGEM
                     SRFSDAAVSKASGEEKDF"
BASE COUNT          391 a         1149 c         1096 g          469 t
ORIGIN      
        1 gcgcgccctg tacccggacc tgtacgacag gtaacggcgg gcgcatgtca aaggacggga
       61 aacgggccgc cttggcctac gcggggctca ttacgctgct cgcggcgtcg ttcgcggccg
      121 ccatcgcgct cggcgcggtc aggctgtcgc cggtcgaggc cgcgcgggcc ttgctttcgt
      181 cgcgcgaagc ctcgtcaccc agggtcgcct cgtcacccga ggcgatcatc gtaggcatca
      241 ggttcgcccg cgcctgcctc gccgcggcgg tcggcgcgtc gctcgccgcc tccggggccg
      301 tgttccaggg cctgttccgc aacccgctcg ccgaccccta cgtgatcggc agctccggag
      361 gcgccgccct aggcgccgtc tgcgcgatag ccttcggctc gggcgctggc gcgctcggct
      421 tctcgggcat cgggctcgcc gccttcgccg gcgggctcgg cgcgaccatg gtcgtctacc
      481 tcgtagccgg cgcgccccgc ctccgttcag acgcctcgag cctgctcctg gccggcaccg
      541 ccctcggctc gctgttctcc gccctcgtat cgatcgtgct ggcgctcaag gacaaggacc
      601 tgcaccaggc ctggttctgg ctgctcggcg gcttctccgg gcgcggcctg cccgagctcg
      661 cggcgtccgc tccggccatg ctggcgggct tcgcggccgc gatggccggg tcccgttccc
      721 tcgacctctt gtcggtcggc gacgaggagg cgaccagcct cggcctcagg ccggccgcga
      781 cccgcttcgt cgtcggcggc ttcgcggctc tgtccgtatc ggccgcggtc gccctgtccg
      841 gcgccatcgg cttcgtcggg ctggtctcgc cgcacatagc caggcgcttc gtcggcccga
      901 ggcacgtacg gctgataccc gcggcggccc tcgtcggcgc ctcgctcctc gtcgtcgcgg
      961 acgtcgtcgc gcgcaccgcc ttcgcgccgg tcgagatgcc ggtcggcgcc atgacggcgc
     1021 tgatcggggc gccgttcttc gtatacaaga tagccaggcg cggactggac ggcgcccgat
     1081 gagcgccctg ctcgaggcgc gcggcctgtc ggccggctac ggcgagcggg tcgtcgtcgc
     1141 gggcgcgtcc ttctccgtat cggccggctc gctgctcgtc gtgctcggcc ccaacggctg
     1201 cggcaagacc accgtgctca agacctgcct cggcctcctg ccgccgctgt ccggcgacgc
     1261 gctgctcggc gggctgccgg taaccggcct gcgtccgcgc gaacgggcgc ggctggcggc
     1321 ctgggtgccg cagctcgcgg agtcggcatg gtcgtaccga tcgcgcgaca tcgtcgcgca
     1381 gggccgattc gccgcgcgcg ggcccttcat gccgttgaac ggcgacgacg aggcggccgt
     1441 cgacgcggcc ctggacgcga tggacgcccg ggccttcgcc gaccggccct tctcgtcgct
     1501 gtccggcggc gaggccaggc gcgtgatgat agccagggcc ctggcgcagg agacgccgct
     1561 catcgtgctc gacgagccgg cggcccacct ggacccgggc cggcagatgg agctgatgga
     1621 gctgctggcc ggcctcgcgc gctccggcaa ggccgtcgcc gtcagcgtcc acgacgtcaa
     1681 cgccgccaag cgcttctccg accttgccct gctcgtcggc aaggacggct cggcggcctt
     1741 cggcgccccc ggcgacgtcc tgacgcccga gaacctcgag aacgcttacg atacccattt
     1801 tatacacggc gaccacgagt cgtacggacc ctacgtactg cccctgtcgc ggaaacgagc
     1861 caaggagagc catgatggac gataagcgga tactgagcga ggtctcggcg ttgctcgacg
     1921 acctgaccaa gcccaagggc agcctgggcc ggctcgagga ctacgcctcg aggctggcgg
     1981 tgatccaggg gcgcgtgccc ccgcgggtca ggcggaaggc ggccttcgtg ctggcgggcg
     2041 accacggcgt cgtctcgcgc ggcgtgtcgc tgtacccgaa agaggtcaca gcccagatgt
     2101 tcgcgaacat catggcgggc ggcgccggca tcaacgtgct gtccagggcc tgcggtttcg
     2161 acgtgttcgt cgtagacgcg ggcgtcgacg ccgatctgcc cgactatccc agcggcggcg
     2221 cggacggcaa gccgcgctac gtcccgatga aggcggtgcg cggcaccagg gacttcaccg
     2281 tcgagccggc ctttaccgag atcgaattcc tccagtgcct gcacaacggc cagaagctgg
     2341 ccgagctagc ggtccgcgag ggctacgaca tcgtcgccct cggcgacatg ggcatcggca
     2401 acacgacgag cgcggccgcg gtgctggccg gctacggctt cgaccccgac ctggtcgtcg
     2461 accggggcac cggcatcgac gacgaggcgc tggaacggaa gcgggcgctc atcacggagg
     2521 cggtggcggc ccgcggcccc tacccgaccc ccgagtcggt cggcgcggcc ttcgccgggc
     2581 cggagatagc cacggccgcg ggcgtgatac tcgggctcaa gggcaagggc gtcgcctgca
     2641 tgctcgacgg cttcccgatc acggccggcg cggtcatagc ttggaagata gacccgagcg
     2701 tcaccgacta cctgttcgcc ggccaccaat ccagggtcaa gggccaccgc ccggtgctcg
     2761 aggcgctcgg cctcgagccg gtcgtgtcgc tcgacatgcg cctgggcgag ggcaccggcg
     2821 ccgtcatcgg aggcttcatc gtcgagctgg gagccaggat agccggcgag atgtcccgct
     2881 tctccgacgc ggccgtctcc aaggcctccg gcgaggagaa ggatttctga gccccgcccc
     2941 gcgcgacgcg gcaggcgaag ggcctggagc agtcctgaag cacggcggcg acttcgtcgc
     3001 gctcgccgag gcttccggcc tgcccgagtc ggccgtcgtc gacgccagcg ccaacctgaa
     3061 cccgctcggg ccgcccgact ggctcgacgc ggccttcgcc gaggg
//
LOCUS       DAOPHV010000304         3104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_43614_length_3104_cov_3.395540, whole genome shotgun sequence.
ACCESSION   DAOPHV010000304 DAOPHV010000000
VERSION     DAOPHV010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43614_length_3104_cov_3.395540"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PLQ29_08430"
     CDS             <1..808
                     /locus_tag="PLQ29_08430"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPG86712.1"
                     /translation="GFDTESINGLLASFRDKAQPMFSKSGILMFNKGRKPESIEERLI
                     AETGRTLLMTSTSSPLPAVDPYPEGRIVTQAMADAFEGPSIFVEGSAFERSRAEKAAV
                     GIVSELYCPILYYQYVVGYIYLMNDSSKKTCLDYRAVDFAWEFSRILAYSLKSNDYFK
                     LSEDSKPDPYTPQVVDLSAGGCLLSMPKSSFKVKLKSGAVLDILIGKDGGGSSVAMKG
                     RVARRFDDRDNEYYGVAFINAEQDAYSELRRSLYADESNRFACDEASLEL"
     gene            821..1066
                     /locus_tag="PLQ29_08435"
     CDS             821..1066
                     /locus_tag="PLQ29_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86713.1"
                     /translation="MDADFSVMGRLRLAMAVASRWETLVTLGAFVAFWLLLRYIADPW
                     RRDDRKPRGVKKAKKPSPTPVEQAIEADESDDDGFPD"
     gene            complement(1023..2543)
                     /locus_tag="PLQ29_08440"
     CDS             complement(1023..2543)
                     /locus_tag="PLQ29_08440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPG86714.1"
                     /translation="MKTDDPIGTYAAEHFGVAYLYPYQRLVIANVLDSAGTATDDDGP
                     TRQIVILPTGSGKSLCFQLPATLCPGPTLVVYPLLGLMADQARRLKSSGMVAATLRGG
                     MSANEKRAALESVRSGTARFIITNPETLRTPSVAKALAEAKPSHFVIDEAHCVAEWGD
                     SFRPAYLSLGEAIRSIKPRVVTAFTATASPPILTRIAEALFGGEPYRLVAGAPDRPNI
                     RYSVKPALSMRRELRAAVATMPKPLIVFARSRHGVQIIAEDLIGSRSGLDCRFYHAGL
                     EKAERAAVEAWFMGSDDGVLVSTCAYGMGMDKPNVRSVVHYGRPASVEAYLQESGRAG
                     RDGEQSEALLIHKLEPGSRTRGDYEPRLGPEPVSAEARSEAMRRYATGCYGCRRSFLL
                     GVLGYPEAAETACSSCDRCDGTAIEDAPGAAELAAIAARHPRRFDATGLAAFAVGAQG
                     TAVAPRHGTMAGWTTEEAGEAIAAALGAGLLRRRSRWPWKGAISPGTRRRPTRRPR"
     gene            complement(2600..>3104)
                     /gene="nth"
                     /locus_tag="PLQ29_08445"
     CDS             complement(2600..>3104)
                     /gene="nth"
                     /locus_tag="PLQ29_08445"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPG86715.1"
                     /translation="QVNKVTPALFEAYPTPGDLAEASLADVESLVRTTGFFRAKAANI
                     VGAATIIARDLGGTVPDTIEELVKLPGVGRKTANLIVSVCFGKPGIVVDTHVLRTARR
                     LGLAPTDDPGKSERMLGAALPESMWTAFSYALNRHGKYVCRARRPLCAECPLAGICPS
                     AADFMAS"
BASE COUNT          524 a         1017 c         1007 g          556 t
ORIGIN      
        1 cgggttcgac accgagtcca tcaacgggct actcgccagc ttccgcgata aggcgcagcc
       61 gatgttctcc aagagcggca tcctcatgtt caacaagggg cgcaagcccg agagcatcga
      121 ggaacggctg atagccgaga cgggccggac gctgctcatg accagcacct cgagcccgct
      181 cccggccgtc gacccctacc ccgagggtcg gatcgtgacg caggccatgg ccgacgcgtt
      241 cgaggggccg tcgatcttcg tcgagggctc cgccttcgaa cggagccgcg ccgagaaggc
      301 cgccgtcggc atcgtctcgg agctgtactg ccccatactc tattatcagt acgtcgtcgg
      361 ctacatttac ctgatgaatg actcgtccaa gaagacctgc ctcgactatc gggccgtcga
      421 tttcgcgtgg gagttctcgc gcatcctcgc ctatagcctg aagtccaacg actacttcaa
      481 gctgtccgag gactcgaagc cggatcccta tacgcctcag gtcgtcgacc tatcggccgg
      541 aggttgcctt ctgtcgatgc cgaagtcgag cttcaaggtc aagctcaaga gcggcgcggt
      601 actcgacatc ctgatcggca aggatggcgg aggctcgagc gtcgccatga aaggccgagt
      661 cgcgagacga ttcgacgacc gggataacga gtattacggc gtcgccttca tcaacgccga
      721 gcaggacgcc tacagcgagc tacgcaggtc gctctacgcc gatgagtcga acaggttcgc
      781 ctgcgacgag gcttctctgg agctgtgatc ggaggttcgg atggatgcag atttctccgt
      841 catgggacgg ctacggctag ccatggccgt ggcctcccga tgggagaccc tcgtcacgct
      901 tggcgccttc gtcgcgttct ggctactcct gcgctatata gcagatcctt ggaggcggga
      961 cgatcgcaag ccccggggcg taaaaaaagc caagaaaccg tctccgactc cggtcgaaca
     1021 ggctatcgag gccgacgagt cggacgacga cgggttcccg gactgatagc ccctttccat
     1081 ggccagcggc ttcggcggcg tagcagaccc gccccgaggg cggccgcgat cgcttctccg
     1141 gcctcttcgg tcgtccaacc ggccatcgtt ccgtggcgcg gcgcgaccgc ggttccctgt
     1201 gcgccgacgg cgaacgcggc caggcccgtc gcgtcgaagc gccgtgggtg acgcgccgcg
     1261 atcgcggcta gctcggcggc gccgggagcg tcttcgatag ccgtcccgtc gcagcggtcg
     1321 caggacgaac aggccgtctc cgccgcctcg ggatagccta ggacccccag caggaacgac
     1381 cttctgcagc cgtagcaccc ggttgcgtag cgccgcatcg cctcggacct ggcctcggcc
     1441 gataccggct ccgggcctag cctcggttca tagtcgcctc gtgtgcggct cccgggctcg
     1501 agcttatgga tgagcagagc ctcgctctgc tcgccgtccc tgccggctcg ccccgattcc
     1561 tggagatagg cctctaccga agccggccgc ccataatgca cgaccgagcg gacgttcggc
     1621 ttgtccatgc ccataccgta ggcgcacgtg cttacgagga cgccgtcgtc ggagcccatg
     1681 aaccatgcct cgacggccgc gcgctcggcc ttctcgagcc cggcgtggta gaagcggcag
     1741 tcgaggcctg agcgcgagcc tatcaagtcc tccgctatga tttggacgcc atggcgggat
     1801 cgggcgaaca cgatcagcgg cttcggcatg gtagccacgg ccgcccgtag ttcccggcgc
     1861 atcgacaggg ccggcttgac gctgtagcgt atgttcggcc tgtcaggagc gcccgcgacg
     1921 agcctgtatg gctcgccgcc gaaaagcgcc tccgcgatgc gcgtgagtat gggaggagag
     1981 gccgtcgcgg tgaaggccgt gacgacgcgc ggctttatgg acctgatcgc ttctccgagc
     2041 gatagatagg ctggcctgaa actgtcgccc cattcggcga cgcaatgggc ctcgtctatc
     2101 acgaagtgcg acggcttggc ttccgctagc gccttcgcca cgctgggcgt ccgtagcgtc
     2161 tctggattgg ttatgatgaa acgggctgtg cccgatcgta ccgattcgag ggcggcgcgc
     2221 ttctcgttcg ccgacatgcc acctcgcagc gtcgccgcga ccatgccgga gctcttgagc
     2281 ctcctggcct gatcggccat gaggccgagc aggggataga cgacgagcgt cggcccgggg
     2341 cagagggtcg ccggtagctg aaagcagagg ctctttccgg aacccgtggg caggatgact
     2401 atctgcctgg tagggccatc atcgtctgtc gccgttcccg ctgagtcgag cacgttcgct
     2461 atgacgaggc gctgatacgg atagaggtag gccactccga aatgttccgc cgcgtacgtc
     2521 ccaatgggat catcggtctt catgcggact atacgccgct cggagcaacg gtgattgtcc
     2581 gcgtcgaccc gcggccgcct cagctagcca tgaaatcggc ggcggagggg catatcccgg
     2641 ccagcgggca ctcggcgcac agaggtctcc tggcacggca gacgtacttg ccgtggcggt
     2701 tgagagcgta ggaaaacgcg gtccacatcg actcgggcag ggccgcgccg agcatccgtt
     2761 cgctcttgcc cgggtcgtcg gtaggggcca gccctagccg cctcgccgtc ctgagcacgt
     2821 gcgtgtcgac gactatgcct ggcttgccga agcagacgct gacgatcagg ttcgccgtct
     2881 tgcggccgac gccgggcagc ttgaccaact cctcgatggt atcgggcacg gttccgccga
     2941 gatcccgggc gatgatggtt gcggctccga ctatattcgc ggccttggcg cggaagaagc
     3001 cggtggtacg tacgagcgac tccacgtccg ccaggcttgc ttcggccaag tctccgggcg
     3061 tcggatacgc ctcgaacagg gcgggcgtca ccttattcac ctgc
//
LOCUS       DAOPHV010000305         3093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_43809_length_3093_cov_3.964450, whole genome shotgun sequence.
ACCESSION   DAOPHV010000305 DAOPHV010000000
VERSION     DAOPHV010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43809_length_3093_cov_3.964450"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2079)
                     /gene="priA"
                     /locus_tag="PLQ29_08450"
     CDS             complement(<1..2079)
                     /gene="priA"
                     /locus_tag="PLQ29_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPG86716.1"
                     /translation="MPSYVSVAFDLPVDRQWSYAEPEGLSAPVGARVEATLGRRAAVG
                     WVVARSDTVDCDPAIVKPYLRLVDAEPLFGPETLRLAEWLSGMYYCSLGEALSAMTPS
                     AERESRRKAKKPASGKGYVGDAGSVGDESSVGDAGSVGDESSVGDAASFDDVRVAPTP
                     LVPSAEQAAALGRVLSGRDGRWYLYGPTGTGKTEVFLEAAEATLAEGRGVIYLVPEIA
                     LTRQVVEAIKDRFGDTCAVIHSGLTGPKKLTEWRRILRGEARVVVGARSAVFAPVSDL
                     GLIVLDEEHEGSYKAGSSPRYHARQVAMRRAGTEGARLVMGSATPSVESWRLMGEGGM
                     ERLVLSRRLAGGDMPAIELVDMRAEGGALSKRLIDEVRRVKREGGQSILFLNRRGFSY
                     FWSCRSCGAEMKCRHCSVGMTYHKERGRLVCHYCGYQAPPPSACPVCGSLDTGWAGFG
                     TERIEEEAARLFPDMSVARLDADATSRKGVLERTLDDFRDGRIDVLLGTQMVAKGLNF
                     PGVRLVGVILADTTLNLPDFRAAERAFGLVTQVAGRAGRFVKGGRVIVQTYRPTSPVL
                     ARAAAHDAAGFYADELAVRRELGFPPYSRLLRVVVRSKEAAAATETAAELAGRIVAAT
                     EAAATSAVTADTTHDKVEVLGPAECPIAVVAGNARRQVLLRSAEAGPGRAALSAALSG
                     WKAPSGVYVEI"
     gene            complement(2085..2813)
                     /locus_tag="PLQ29_08455"
     CDS             complement(2085..2813)
                     /locus_tag="PLQ29_08455"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPG86717.1"
                     /translation="MGNDDRIYCYEALSLMEDALADGYRSRSPVPEFADQAESARAPE
                     ARTTQGPARIPQDDSLAGIAAEAARCMACRLGAGRKRSVPGQGPARPLVMVIGEGPGS
                     EEDKTGLPFVGPAGQLLDKMLSSIGLSRELNCFIGNIVKCRPPMNRDPAPDEQAACRP
                     YLDRQLALLRPKVILAVGRIPAQSLLGVKDGIGRLRGRAYEFEGRPLVATYHPSALLR
                     DEGLKRPAWEDLKLLRSLLDGLGA"
     gene            complement(2820..>3093)
                     /locus_tag="PLQ29_08460"
     CDS             complement(2820..>3093)
                     /locus_tag="PLQ29_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86718.1"
                     /translation="PNYERYSELLTMRSGSLFGALSFLDGARRDMRTTARERSLVLRF
                     HGPLLKAVSVSNPAAGRAVYRLLGAAAARNARDISIEMRNLLAERA"
BASE COUNT          444 a         1104 c         1076 g          469 t
ORIGIN      
        1 tatctccacg tagacgccgg acggcgcctt ccagcccgat agggccgccg ataacgcggc
       61 cctgcccggg ccggcctcgg cggaccgtag cagtacctgc ctgcgggcat tgcccgcgac
      121 caccgctatg gggcactcgg ccgggccgag tacctcgacc ttgtcatgcg tcgtgtccgc
      181 cgttaccgcg ctagtcgccg ccgcctcggt agcggcgact atcctgcccg ccagctcggc
      241 ggccgtctcg gtagcggcgg cggcttcctt cgacctgacc accacccgta gcagcctcga
      301 gtagggcggg aagcccagct cccgcctgac ggccagctcg tccgcataga agcctgccgc
      361 gtcatgggcc gcggcgcgag cgagcaccgg gctcgtcggc cggtaggtct gcacgatcac
      421 ccgaccgccc ttgacgaagc ggccggcgcg gccggctacc tgcgttacca ggccgaacgc
      481 gcgctccgcc gccctgaagt ccggcaggtt aagggtcgtg tcggcgagta tcaccccgac
      541 gagccgtacg ccggggaaat tgaggccctt ggcgaccatc tgggtgccga gcagtacgtc
      601 tatcctgccg tcgcggaagt cgtcgagggt gcgctcgagc acgcccttgc gcgaggtcgc
      661 gtcggcgtcg agcctggcca cgctcatgtc ggggaagagc ctcgcggctt cctcctcgat
      721 gcgctcggtg ccgaagcctg cccagccggt gtcgagcgag ccgcagacgg gacaggccga
      781 aggcggaggc gcctggtagc cgcagtagtg gcagacgagg cggccccgct ccttatggta
      841 ggtcatgccg accgagcagt gacggcactt catctcggcc ccgcagctcc ggcagctcca
      901 gaagtatgag aagccgcgac ggttgaggaa caggatggac tggccgcctt cccgcttgac
      961 ccgtctgacc tcgtcgatca gtcgtttcga gagggcgccc ccctcggccc tcatgtccac
     1021 gagctctatc gccggcatgt cgccgccggc cagccggcgt gacaggacga gccgctccat
     1081 gccgccctct cccatcagcc gccacgactc gacgctcggc gtggccgagc ccatgaccag
     1141 cctggcgcct tccgtgccgg cgcggcgcat ggccacctgg cgggcgtggt agcgcggcga
     1201 cgagccggcc ttgtacgagc cttcgtgctc ctcgtcgagc acgatcagtc cgaggtcgct
     1261 gaccggagcg aatacggcgc tacgggcgcc tacgacgact ctggcctcgc cgcgcaggat
     1321 gcgccgccat tcggtcagct tcttgggacc ggtcaggccc gagtggatga ccgcgcaggt
     1381 atcgccgaat cggtccttga tcgcttcgac cacctgccgc gtcagcgcta tctcgggcac
     1441 gaggtagatg acgccgcgac cctcggcgag ggtcgcctcg gcggcctcga ggaagacctc
     1501 ggtcttgccc gtgccggtgg ggccgtacag gtaccagcga ccgtcgcggc cggacagcac
     1561 gcgcccgagc gcggcggcct gctcggccga cggcacgagc ggggtcgggg cgacgcgcac
     1621 gtcgtcgaac gaagcggcgt ctcctacgga gctctcgtct cctacggagc cggcgtctcc
     1681 tacggagctc tcgtctccta cggagccggc gtctccgacg tagcccttcc ccgaagccgg
     1741 cttcttggcc ttgcgccggc tctcccgctc ggccgacggc gtcatcgccg acagcgcctc
     1801 gccgagcgag cagtagtaca tgccggatag ccactccgcg agcctgagcg tctccgggcc
     1861 gaacaggggc tcggcgtcga cgaggcgcag gtacggcttg acgatggccg ggtcgcagtc
     1921 gacggtatcg ctcctggcga cgacccagcc gaccgcggcc ctgcggccga gcgtcgcctc
     1981 gacccgggcg cctacgggcg cggacaggcc ctcgggctcc gcgtagctcc actgcctatc
     2041 gacgggcagg tcgaaggcga ccgagacgta gcttggcacg ggcgctaggc cccgaggccg
     2101 tcgagcagcg atcgcagcag cttgagatct tcccaggccg ggcgcttcag gccctcgtcg
     2161 cgcaggaggg cgctcgggtg gtaggtggcg acgagcggtc gtccctcgaa ctcgtaggcc
     2221 cgaccgcgca gcctgccgat gccgtccttg acgccgagca acgactgggc cggaatgcgg
     2281 ccgaccgcca gtatgacctt gggcctgagc agggccagct ggcggtccag gtaggggcgg
     2341 caggcggcct gctcgtccgg cgccgggtcg cggttcatcg gagggcggca cttgacgatg
     2401 ttgccgatga agcagttcag ctcccgagac agcccgatcg atgacagcat cttgtcgagg
     2461 agctggccgg ccgggccgac gaagggtagg ccagtcttgt cctcctccga gcccggaccc
     2521 tcgccgatga ccatgacgag gggccgggcc ggaccctggc cgggtaccga gcgcttgcgg
     2581 ccggcgccga ggcggcaggc catgcagcgg gcggcttcgg ccgcgattcc cgccaggcta
     2641 tcgtcctggg gaatccgcgc cggaccctgg gtcgttcgtg cctcgggggc ccgggccgac
     2701 tcggcctggt cggcgaattc cgggacgggc gaacggctcc ggtagccgtc ggccagcgcg
     2761 tcctccatca gcgataatgc ctcgtagcag tatatcctgt cgtcgtttcc catcgtcgct
     2821 tacgccctct cggccagcag attgcgcatc tctatcgaga tatcgcgcgc gttcctggcc
     2881 gccgccgccc cgagcagccg atagaccgcc ctgccggccg ccgggttgga gacgctgacc
     2941 gccttgagca gggggccgtg gaagcgtagc accagcgaac gctcgcgggc ggtcgtcctc
     3001 atgtcccgac gagcgccgtc gaggaaggac agggcgccga ataaggagcc ggaccgcatc
     3061 gtgagcagct ccgagtagcg ctcgtagttc ggt
//
LOCUS       DAOPHV010000306         3093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_43813_length_3093_cov_2.882818, whole genome shotgun sequence.
ACCESSION   DAOPHV010000306 DAOPHV010000000
VERSION     DAOPHV010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43813_length_3093_cov_2.882818"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLQ29_08465"
     CDS             complement(<1..208)
                     /locus_tag="PLQ29_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86719.1"
                     /translation="MMDDTDLRGLSAEDAKAYALEFMTSLKTLERQLAGLGDELGIWA
                     KRVELAAAKGAADLETAARARLAEL"
     gene            complement(213..1547)
                     /locus_tag="PLQ29_08470"
     CDS             complement(213..1547)
                     /locus_tag="PLQ29_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86720.1"
                     /translation="MKRTSTAAIVALLLVAAATAAFAQPEVSEKKEIAIFSLGFYGWD
                     IPRETLGTIDIDIQRVFLDLGRFTIFGMEKRFSTSDVDQFITILKQMKESTFVLPEKY
                     QFGEAFLTEADFNRLVGSFIIAVPVVTSYNSQYVDGKEWRTDIKTNVSFINVADGTMI
                     GIANVETQGSSRETQYTSVKEAIDGIPMQLQYEIRKISQFQNVTRVLASGFGGVDIQL
                     GSNMGIKKGDEYAVIVVDELEGFVDEREMGLIVIKDVGTTRSKGTILYSKAKVEKDTQ
                     LCEIPRLGADLAVYAHTYKYFSGGSGNDSSFVIGARTEMTRGFYGFRPYAAIQAIADF
                     EMWMPINVIVGGQVNTFIRRIEIGARAGVGGSTNAIIRLIEEQLSESDDPWFTHYGVS
                     AGAYLSYLLSRDFKVFIEAQGDYMLGILDPTPGGLFGSYGGLQLGVGATFKL"
     gene            complement(1637..2263)
                     /locus_tag="PLQ29_08475"
     CDS             complement(1637..2263)
                     /locus_tag="PLQ29_08475"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPG86721.1"
                     /translation="MIEPLRISLPTLKRLPLYRRILEEKAAAGDEWVSSDALAKRLGQ
                     CAVQVRKDLAQAGLVGKAKYGFPVAQSIVTLDAALGSASSRDIFVIGAGPMADAALAD
                     ESLAAHGFKAVAVFEPDPALVGASVGRDGVRALPLSKLPDLSRRMGVKIAILAVAGAW
                     AQAAADGLKDSGIEAVLDLSPAHPQFPAGVRSVRADFGAAIAALAAGL"
     gene            complement(2401..>3093)
                     /locus_tag="PLQ29_08480"
     CDS             complement(2401..>3093)
                     /locus_tag="PLQ29_08480"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPG86722.1"
                     /translation="TEKHIKYPIVAADMVPDLAILDPALPATMPASVTANTGMDVLCH
                     AIEAVASTAATSFTDPYAVEAIKLVLAYLPKAFSSPKDMDARFHMHNASALAGMAFTN
                     ASLGLVHSLAHKIGGEFGVTHGLANAILLPYIIDFNRKSSAKYGKVERDLGVADLADE
                     VRKLNAKLGIPATFKDCAEVPFEEAKFMAVLDRMSANAHADPCTLTNPGAPTVADVRR
                     IYEAAYYGRSMK"
BASE COUNT          505 a          949 c         1067 g          572 t
ORIGIN      
        1 ctagctcggc gagcctggcc cgagccgccg tctcgaggtc cgcggcgccc ttggccgcgg
       61 ccagctccac ccgcttggcc cagataccca gctcgtcgcc gaggccggcg agctggcgct
      121 cgagcgtctt gagcgaggtc atgaactcga gggcgtaggc cttggcgtcc tcggccgaca
      181 gcccccgtag atcggtatcg tccatcatcg ccctagagct tgaaggtggc gccgacgccg
      241 agctggagtc ctccgtagga gccgaagagg ccgccgggcg tcgggtcgag gatgccgagc
      301 atgtagtcgc cctgggcctc gatgaagacc ttgaaatcgc gcgagagtag gtacgatagg
      361 taggcgccgg ccgagacgcc gtagtgcgtg aaccacgggt cgtcgctctc ggacagctgc
      421 tcctcgatga ggcggatgat cgcgttggtc gaaccgccga cgcctgcgcg ggcgccgatc
      481 tcgatgcggc ggatgaacgt gttgacctgc ccaccgacga tcacgttgat cggcatccac
      541 atctcaaagt cggcgatggc ctggatggcg gcgtacggcc tgaatccgta gaagccgcgc
      601 gtcatctcgg tacgcgcccc tatcacgaac gacgagtcgt ttccggagcc gcccgaaaag
      661 tacttgtacg tgtgggcgta gactgccagg tcggcgccga gacgcggaat ctcacacagc
      721 tgcgtgtcct tctcgacttt ggccttggag tagaggatgg tgcccttgga acgggtcgtt
      781 ccgacgtcct tgatgacgat gaggcccatc tcgcgctcgt ccacgaagcc ctcgagctca
      841 tcgacgacga tgacggcgta ctcgtcgcct ttcttgatgc ccatgttgga gccgagctgg
      901 atatcgacgc cgccgaagcc ggaggcgagc acccgcgtga cgttctggaa ctgggatatc
      961 ttgcggatct cgtactgcag ctgcatcggg atgccgtcga tggcttcctt gaccgacgta
     1021 tactgagttt cgcggctcga gccttgagtc tcgacgttgg cgatgcctat catcgtgcca
     1081 tcggccacgt tgatgaagct gacgttcgtc ttgatgtcgg tgcgccattc cttgccgtcg
     1141 acatactggc tgttgtagct cgtgacgacc ggcaccgcga tgatgaacga gccgacgagg
     1201 cgattgaagt cagcctcggt caggaacgcc tcgccgaact ggtacttctc tggcagcacg
     1261 aaggtggatt ccttcatctg cttcaggatg gtgatgaact ggtcgacgtc gctcgtggag
     1321 aaacgcttct ccatgccgaa gatggtgaag cggccgaggt cgaggaagac ccgctggatg
     1381 tcgatgtcta tcgtgccgag cgtctcccgc gggatgtccc agccgtagaa gccgagcgag
     1441 aagatggcta tctctttctt ctcgctgacc tcgggctgcg cgaaggccgc cgtggcggcg
     1501 gcgacgagta gcagggcgac gatagccgcc gtcgatgtac gtttcatgat ccctcctgat
     1561 ggccgagtgt gcctagctcg actagtatag agcatgacgg acgcttttgc gagcggtgac
     1621 gatacgggcc cgagggctac agccccgccg ccagcgcggc tatagccgcc ccgaagtcgg
     1681 cccggacgga tcgtacgccg gccgggaact ggggatgcgc gggcgacagg tcgagcaccg
     1741 cctcgatgcc gctgtcctta agcccgtccg cggccgcctg ggcccaggcg ccggcgacag
     1801 ccaggatcgc tatcttgacg cccatgcgcc tggacaggtc tggcagcttc gataggggta
     1861 acgccctgac gccgtcccgg ccgaccgatg ccccgaccag ggccggatcc ggctcgaaga
     1921 ccgctaccgc cttgaaaccg tgggccgcca ggctctcgtc ggctagggcc gcgtcggcca
     1981 tcgggccggc cccgatcacg aagatgtccc gcgacgacgc cgagccgagg gccgcgtcca
     2041 gggtgacgat ggactgagcc accgggaagc cgtacttggc cttgccgacg aggccggcct
     2101 gggcgaggtc cttgcgcacc tggacggcgc actgcccgag ccgcttggcg agcgcgtcgg
     2161 agctgaccca ctcgtcgccc gcggcggcct tctcctcgag gatgcgcctg tacagcggta
     2221 gccgcttgag cgtcggtagc gatattcgca ggggttcgat catcggcgcc agtataggtt
     2281 ccttccttgc tctttcgcaa tggttatgtt tatatcggaa tccgtccggt cggggacggg
     2341 ccgaaaaaaa agaagccgcg ccgatcgggg ggatgaccgg cgcggcttcg gtacagagac
     2401 ttatttcatc gagcggccgt agtacgcggc ctcgtagatg cgcctgacgt cggcgacggt
     2461 cggcgcgccg gggttggtca gcgtgcaggg gtcggcgtgg gcgttggcgc tcatcctgtc
     2521 gagcacggcc atgaacttgg cctcctcgaa gggcacctcg gcgcaatcct tgaacgtggc
     2581 ggggataccg agcttggcgt tgagcttacg cacctcgtcg gcgaggtcgg ccacgccgag
     2641 gtctcgctcg accttgccgt acttggcgct cgacttcctg ttgaagtcga tgatgtacgg
     2701 caggagtatc gcgttggcca ggccgtgggt aacgccgaac tcgccgccta tcttgtgagc
     2761 cagcgagtgg acgaggccga gcgacgcgtt cgtgaaggcc atgccggcca gggccgacgc
     2821 gttgtgcatg tggaagcggg cgtccatgtc cttgggcgac gagaaggcct tgggcaggta
     2881 cgcgagcacc agcttgatcg cctcgacggc gtaggggtcg gtgaacgagg tggcggcggt
     2941 cgaggctacg gcctcgatgg cgtggcagag cacgtccatg ccggtgttcg ccgtgacgga
     3001 ggcgggcatc gtggccggca gggccgggtc gagtatggcg aggtcgggca ccatgtccgc
     3061 ggcgacgatc gggtacttga tgtgcttctc ggt
//
LOCUS       DAOPHV010000307         3091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_43849_length_3091_cov_3.918643, whole genome shotgun sequence.
ACCESSION   DAOPHV010000307 DAOPHV010000000
VERSION     DAOPHV010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43849_length_3091_cov_3.918643"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /locus_tag="PLQ29_08485"
     CDS             complement(<1..1638)
                     /locus_tag="PLQ29_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPG86723.1"
                     /translation="MAARAPRPLPEYLAGLNPEQLEAVLHEDGNLLILAGAGSGKTRV
                     ITTKIAYLIAERGLAPESILAVTFTNKAAAEMRERACAIEPACARANLRTFHSFGAWF
                     LRRNAAAAGLDRDFTIYDDDDSTTLLRAAFPELSRPEAGKLASAIARAKDYGLTPDAP
                     DLERGFGGKDFRRRYAEYEGRLRQTGNVDFGDLISLPVRVLEREPAIRERVHARFRVI
                     MVDEYQDSNVAQFELLKALAGPEAYVCVVGDDDQSIYRFRGAEVKNILSFPEVFPRTA
                     VVKLERNYRSYQSILDIASRVVENNEGRLGKTLRATRTGGEKPTLAVLRDHDEETAYC
                     IRVIDAHLRTGGSFSDVAILYRTNAQSLAFERAFPPAGIPYRLVGALRFYEREEVKDA
                     LALLALASNPRDEVAFRRVANKPARGVGDASLEAVVEAAFLGDGDLVAAAAEARAKSA
                     KARAGLAAFAALMQKLAERLAAPALADDGGRALDDDAPGPSGPPPVAPGAERLSAVVE
                     YAVRESGLLQYHKDQDDIAGSQKTANLDELVNAASDYP"
     gene            complement(1628..2944)
                     /gene="rimO"
                     /locus_tag="PLQ29_08490"
     CDS             complement(1628..2944)
                     /gene="rimO"
                     /locus_tag="PLQ29_08490"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPG86724.1"
                     /translation="MKSFYVDNHGCAKNQVDAEEMSARLVSAGWSASSTIADADLVIV
                     NTCGFIEDAKKESIEAVLTLKAAYPGKRLLVAGCLAQRYAEALGDDLPEADGIFGNGD
                     LSLASQAAEAALAGHRPNLAPERGPYEPARRGGLLGFPRSAYLKIAEGCDNRCSFCAI
                     PLIRGGLSSRDPDDVAAEFAELVAAGAYEVCIIGQDLGSYGLDRAGRQLLPELLASIS
                     SISGDWQARMLYIHPDRFPSGAIDACSRDARILPYFDLPFQHASRRVLRAMNRSGDAE
                     RYLGLLSDIRSALPDAVIRSTFLVGFPGESDDEFAELRAFQDAARVDWLGAFSYSREE
                     DTPAYSMPGRVSKRVAAARKKAVEDAQRPITEASLGRFVDTAQTALVEERIEGDDLAI
                     ARGYMNAPDVDGSIVVVGGDLAPGDVVPVTITAVRGVDLEAVPRGR"
BASE COUNT          454 a          998 c         1156 g          483 t
ORIGIN      
        1 cgggtagtcg ctcgcggcgt tgaccagctc gtcaaggttg gccgtcttct gggagccggc
       61 tatgtcgtcc tggtccttgt ggtactggag gagccccgac tcgcgcaccg cgtattcgac
      121 gacggcggac agccgctcgg cacctggcgc cacgggcgga gggccgctcg gacccggcgc
      181 gtcgtcgtcc aaggcccgcc cgccatcgtc ggccagggct ggcgcggcga gccgctcggc
      241 gagtttctgc atcagagcgg cgaaggcggc gagccccgcg cgggccttgg ccgacttggc
      301 ccgggcctcc gctgccgccg cgacgaggtc gccgtcgcca aggaacgccg cctcgacgac
      361 ggcctcgagg ctcgcgtcgc cgacgcccct cgccggcttg ttggcgacgc gcctgaaggc
      421 cacctcgtcc cgtgggttcg acgccagggc caggagcgcg agcgcgtcct tgacctcctc
      481 acgctcgtag aagcgcaagg cgccaaccag gcggtacggg atgccggccg gcgggaaggc
      541 ccgctcgaag gcgagggact gggcattcgt acggtatagg atggccacgt cggagaacga
      601 gccgcctgta cgcaggtggg cgtctatcac gcggatgcag taggcggtct cctcgtcgtg
      661 atcgcggagc accgcgaggg tcggcttctc tccgcccgtc ctcgtcgcgc ggagcgtctt
      721 cccgaggcgg ccttcgttgt tctcgacgac gcggctggct atgtcgagga tgctctggta
      781 ggagcggtag ttgcgttcga gcttgacgac ggccgtgcgc gggaagactt cggggaagct
      841 caggatgttc ttgacctcgg ctcctcggaa gcgatagatg ctctggtcgt cgtcgccgac
      901 gacgcagaca taggcctcgg gaccggcgag cgccttgaga agctcgaact gggccacgtt
      961 cgagtcctgg tactcgtcga ccatgatcac gcggaagcgc gcgtggacac gctcgcgtat
     1021 cgccggctcg cgctcgagca ccctgaccgg cagggagatg aggtcgccga agtcgacgtt
     1081 gccggtctgc cgtagccggc cctcgtactc ggcatagcgg cggcggaagt ccttgccgcc
     1141 gaagccgcgc tcgaggtcgg gcgcgtcggg cgtcaggccg tagtccttgg ctcgggcgat
     1201 ggccgaggcc agcttgcccg cctccggcct ggatagctcg gggaaggcgg cgcggagcag
     1261 ggtggtcgag tcgtcgtcgt cgtagatcgt gaagtcgcgg tccagcccgg cggccgcggc
     1321 gttgcgcctt aagaaccagg cgccgaacga gtggaaggta cgcaggttgg ccctggcgca
     1381 ggccggctcg atggcgcagg ctcgctcgcg catctcggct gccgccttat tggtgaaggt
     1441 gaccgccagg atcgactcgg gcgcgaggcc gcgctcggct atcaggtagg ctatcttcgt
     1501 ggtgatcaca cgggtcttac ccgagccggc gccggcgagg atcagcaggt taccgtcctc
     1561 atgcagcacg gcctcgagct gttcgggatt gaggccggcg agatactcgg gaaggggcct
     1621 cggggcccta gcggccacgg ggcaccgcct cgaggtcgac gccccgcacc gccgtgatgg
     1681 tcaccggtac gacgtcgccg ggggccaggt cgccgcccac gacgactatg gagccgtcga
     1741 cgtctggcgc gttcatgtag ccgcgggcta tggccaggtc gtcgccctcg atgcgctcct
     1801 cgacgagcgc cgtctgggcg gtatcgacga agcgcccgag cgacgcctcg gtgatggggc
     1861 gctgggcgtc ctcgacggcc ttcttgcggg cggcggcgac gcgcttggac acccggcccg
     1921 gcatcgagta ggccggagta tcctcctcgc ggctgtagga gaaggcgccg agccagtcga
     1981 cgcgggccgc gtcctggaag gcgcgcagtt cggcgaactc gtcgtcgctc tcgcccggaa
     2041 agccgaccag gaaggtcgag cggatcaccg cgtcgggtag ggcggagcgt atgtcggaga
     2101 ggaggccgag atagcgctcg gcgtcgcccg agcggttcat cgcgcggagc acgcgacggg
     2161 aagcgtgttg gaacggtagg tcgaagtagg gcaggatgcg cgcgtcgcgc gagcaggcgt
     2221 ctatcgcgcc cgaggggaag cggtcaggat ggatgtacag catcctggcc tgccaatcgc
     2281 ccgatatgga cgagatcgag gcgagcagtt ccggaaggag ctgccgcccg gcgcggtcca
     2341 ggccgtagga gccgaggtcc tgcccgatga tacagacctc gtaggcgccg gccgccacga
     2401 gctcggcgaa ctcggccgcg acgtcgtcgg ggtcgcggga cgatagcccg ccgcggatca
     2461 gcgggatggc gcagaaggag cagcggttat cgcaaccctc ggctatcttg aggtaggccg
     2521 agcgcgggaa gcccaagagc cctccgcggc gggccggctc gtagggaccc cgctcgggcg
     2581 cgaggttggg gcgatgcccc gcgagcgccg cttcggcggc ctgggaggcg agggacaggt
     2641 cgccgttgcc gaagataccg tcggcctcgg gcagatcgtc gcccagggcc tcggcgtagc
     2701 gttgggccag gcaaccggcg acgagtaggc gcttgccggg ataggcggcc ttgagcgtca
     2761 ggacggcctc gatcgattcc ttcttggcgt cctcgataaa accgcaggtg ttgacgatga
     2821 cgaggtccgc gtcggcgatg gtcgacgagg cggaccaacc ggcggagacg agcctggcgc
     2881 tcatctcctc cgcgtcgacc tggttcttgg cgcagccgtg gttgtcgacg tagaacgact
     2941 tcaattgacg tcgtacacgc cgaggctcca ggtgccgtcc tcggcctggg accagcgtat
     3001 ctgcttgacg gccacctcac ccggggcccc gagttcgaca tcggcgctct ggcctccgga
     3061 ggcctggatg gtcagcttgc aggctgcggc g
//
LOCUS       DAOPHV010000308         3085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_43954_length_3085_cov_3.818812, whole genome shotgun sequence.
ACCESSION   DAOPHV010000308 DAOPHV010000000
VERSION     DAOPHV010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43954_length_3085_cov_3.818812"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PLQ29_08495"
     CDS             <1..889
                     /locus_tag="PLQ29_08495"
                     /inference="COORDINATES: protein motif:HMM:NF040334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6144 family protein"
                     /protein_id="HPG86725.1"
                     /translation="ERSVPRGSSLPGLRLAELDAAIASMIGQLDEGALMALARYFAVI
                     GHDDASIRLLAYLLPVGVLEAMGERLGSLEGDDRARRVMSRLSIPPVGSPPEAYPAAT
                     ARFVSALLDELGAGPATRVLAWNVHGIPVSAFAAERDRLRSLGSIDAWLRDYHDRQVA
                     ELARHAADGTLWFEQRITPEVVEFVRANQEIQGGVRVGDSIFVTKIPYDPDRFLRSGD
                     ALERRRLACHCPLAASSITAAGAGVDAAWCACSAGFTKFMFDVAFGEETEAEVLSSVL
                     AGDPLCRFAIRVPASYAVE"
     gene            complement(1047..2243)
                     /gene="ygeW"
                     /locus_tag="PLQ29_08500"
     CDS             complement(1047..2243)
                     /gene="ygeW"
                     /locus_tag="PLQ29_08500"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HPG86726.1"
                     /translation="MGVNEIKALIDQLSKKKIDMYHKDFFLTWEKSRDEIDATFLVAD
                     VLRSMREANISPKIWDTGIAVSNFRDNSTRTRFSFASAADLLGLYVQDLDEGKSQIAH
                     GETVRETANMISFLSEAIGIRDDMYLGEGDKYQREVAQALDDGVAAGVLPDRPTVVNL
                     QSDIDHPTQSMADFLHLAHHFGGVDKLKGKKIAMTWAYSPSYGKPLSVPQGIIGLASR
                     FGMEISLAHPEGYDLIPEVVELSKKQAAASGGSFKHTNSMDEAFKDADIVYPKSWAPY
                     DVMLQRVPLLHAGNQGGLKDLEKACLANNARFKDWECTEEKMKLTKGGKALYMHCLPA
                     DITDVSCAAGEVAATVFDRYRDDTYREASYKPYIIAAMILLGKRKDPAATLRALLERK
                     QIREVM"
     gene            complement(2446..>3085)
                     /locus_tag="PLQ29_08505"
     CDS             complement(2446..>3085)
                     /locus_tag="PLQ29_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPG86727.1"
                     /translation="LRDKCLKIAAGLLGEPVEDLELVHRGVVRGKKGEITLAKLAHMA
                     TQGEGHGDIVGFGGFKTDHTAFPYGAHFAEVAVNRRTGEVRIRRFHAYQDCGTPINPG
                     LAKGQIYGGVMKAVGHSLYEELQFDELGRPLNLGFTDYKIPSVYEVPEDFHVELVPVE
                     DEVGPFGGKSVSEISLNSAAPAISIAIHDAVGAWCRTWPFTPERVLRAMGRL"
BASE COUNT          456 a          988 c         1042 g          599 t
ORIGIN      
        1 cgaacgctcc gttccgcgcg gatcgagcct gcctgggcta aggctggctg agctcgacgc
       61 ggccatcgcg agtatgatcg gccagctcga cgagggcgcg ctcatggccc tggcccggta
      121 cttcgcggtg atcgggcacg acgacgcgtc gatacggctt ctggcgtacc tcctgccggt
      181 cggcgtgctc gaggcgatgg gcgaacggct cggctcgctc gagggcgacg accgggcccg
      241 gcgagtcatg tccaggctgt ccatcccacc ggtcggctcg ccgcccgagg cctatccggc
      301 ggcgaccgct cgcttcgtat cggccctgct cgacgagctg ggggctggac ccgcgacgag
      361 ggtgctggcc tggaatgtcc acggcatacc cgtctcggcc ttcgccgcgg agcgcgatag
      421 gctgcggagc ctcggctcga tcgacgcgtg gctccgcgac taccacgacc ggcaggtagc
      481 cgagctggcg cggcacgccg ctgacggcac gctgtggttc gagcagagga ttacacccga
      541 ggtggttgaa ttcgtgcggg cgaaccagga gatccagggc ggcgtacgcg tcggcgacag
      601 tattttcgtg acgaagatac cctacgaccc ggaccgtttc ctgaggtcgg gcgacgcgct
      661 ggagcgacgc cgcctggcct gtcactgccc gctcgccgcg tcgtctatta ccgcggcggg
      721 agccggagtc gacgcggcct ggtgcgcctg ctccgcgggc tttactaaat tcatgttcga
      781 cgtggccttc ggcgaggaga ccgaggccga ggtcctgtcg tcagtgctgg ccggcgatcc
      841 gctatgccgg ttcgcgatac gggtgcccgc ctcgtacgcc gtggaatagg cgcgcctacg
      901 gatccgagct cgcccggtac tggacccggg ctcgcccgct actggacccg ggcggggcga
      961 cggatagccg ttaccggcca tccgtcgcct ccgtcctcgc ccactccttt tttattacat
     1021 cgcggccggg gttgcccggc cctcgcttac atgacctcgc ggatctgctt gcgctcgagc
     1081 agggccctga gcgtggcggc agggtccttg cgcttgccga gcagtatcat cgcggcgatg
     1141 atgtacggct tgtagctggc ctcgcggtag gtgtcgtcgc ggtagcggtc gaacacggtc
     1201 gctgcgacct cgccggcggc gcaggagacg tcggtgatgt cggccggcag gcagtgcatg
     1261 tacagcgcct tgccgccctt ggtcagcttc atcttctcct cggtgcactc ccagtccttg
     1321 aagcgggcgt tgttggccag gcaggccttc tcgaggtcct tgagcccgcc ctggttgccc
     1381 gcgtggagta gcggcacgcg ctgcagcatc acgtcgtagg gtgcccagct cttcgggtag
     1441 acgatgtcgg cgtccttgaa cgcctcgtcc atcgagttag tgtgcttgaa cgaaccgccg
     1501 gaagcggcgg cctgcttctt ggacagttcg acgacctcgg ggatcaggtc gtagccctcg
     1561 gggtgagcga gggatatctc catgccgaag cggctggcca gcccgatgat gccctggggc
     1621 accgacagcg gcttgccata ggacggggaa taggcccagg tcatagcgat cttcttgccc
     1681 ttgagcttgt cgacgccgcc gaagtggtgg gccaggtgga ggaagtcggc catcgactgg
     1741 gtcgggtggt cgatgtcgct ctgcaggttg acgacggtcg gcctgtccgg tagcacgccg
     1801 gcggcgacgc cgtcgtccag ggcctgggct acctcgcgct ggtacttgtc gccctcgccg
     1861 aggtacatgt catcgcgtat gccgatcgcc tcggacagga agctgatcat gttggcggtc
     1921 tcgcgcaccg tctcgccgtg ggctatctgg ctcttgccct cgtcgaggtc ctggacgtac
     1981 aggccgagga ggtcggcggc gctggcgaaa gagaagcggg tgcgggtcga gttgtccctg
     2041 aaattcgaga cggcgatgcc ggtgtcccag atcttggggc tgatgttggc ctcgcgcatc
     2101 gagcggagga cgtcggcgac gaggaacgtc gcgtcgatct cgtcgcggct cttctcccag
     2161 gttaggaaga agtccttgtg gtacatgtcg atcttctttt tcgacagctg gtcgatcagt
     2221 gccttgatct cgttaacgcc catgcgtaac ctccctacga ttcataccgg cccttattga
     2281 gccggctttt cattgcttgc gaactactaa ccacggaggc acggagccac ggagtcgaga
     2341 aggaaaagga gtatttgaga aacccaatct cgcattaatc cataacccat gtatctcccc
     2401 ctttctccgt gtctccgtgc ctccgtggtt caaaatccct atcctctaca gtctacccat
     2461 cgcgcggagg acgcgctcgg gggtgaacgg ccaggtccgg caccaggctc cgacggcgtc
     2521 atggatcgct atcgatatgg ccggcgcggc cgaattgagc gatatctccg atacgctctt
     2581 gccgccgaag gggccgacct cgtcctcgac cggtacgagc tccacgtgga agtcctcggg
     2641 cacctcgtag acgctcggga tcttgtagtc ggtgaacccg aggttgagcg gcctgcccag
     2701 ctcgtcgaac tggagctcct cgtacagcga gtggcccacg gccttcatga cgccgccgta
     2761 gatctggccc ttggccaggc cgggattgat cggcgtgccg cagtcctggt aggcatggaa
     2821 ccgacgtatc ctgacctcgc cggtgcgccg gttgacggcg acctcggcga agtgcgcccc
     2881 gtacgggaac gcggtgtggt ccgtcttgaa gccgccgaag ccgacgatgt cgccgtggcc
     2941 ctcgccctgg gtggccatgt gggccagctt ggcgagggtg atctcgccct tcttgccgcg
     3001 tacgacgccg cggtgcacca gctccaggtc ctcgaccggc tctccgagca ggccggcggc
     3061 tatcttgagg cacttgtcgc ggagg
//
LOCUS       DAOPHV010000309         3079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_44068_length_3079_cov_3.883598, whole genome shotgun sequence.
ACCESSION   DAOPHV010000309 DAOPHV010000000
VERSION     DAOPHV010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44068_length_3079_cov_3.883598"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLQ29_08510"
     CDS             complement(<1..275)
                     /locus_tag="PLQ29_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86728.1"
                     /translation="MSSTDDTILASNAAYTDISDAPFTINTASGASIKVESPNGGEIW
                     TQGSTQVISWSSTGSPGPTVKIEVLKGTSILKTLKDIPTTSGSISVPV"
     gene            274..471
                     /locus_tag="PLQ29_08515"
     CDS             274..471
                     /locus_tag="PLQ29_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86729.1"
                     /translation="MSVFLPVIGTWIGSRSFSVEPIRMFFHKWPRNFALSLGVEALVA
                     QPIARQVLLRLHLRKDARASL"
     gene            576..1991
                     /locus_tag="PLQ29_08520"
     CDS             576..1991
                     /locus_tag="PLQ29_08520"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPG86730.1"
                     /translation="MKRLALVLVAICALPLGADELEDALYRSVDALSQAYRKAHPDLG
                     MRAGLAMLDFEEQSELARRHGLGTALESYVRDTVERSLVYELVDRRRMSDILAELELS
                     LSGLVDGRDAVEAGRIAGVRAFLWGGIAELGDSFVISLGVTDAETGLVLATESFELPK
                     RLLVSAAEELAYSYVAPNGIGLSVHGFAPLFQLSDLYTKSSLFFADLGFSYRPNRHFM
                     VSGGIMTAPSLTGEHYRVDEDIDVLSVQPELAATVDPWGLAGSTANAGFSQWTARFRA
                     TFIRLDAQYTVNFSPRFNIGLAGGIFNALGNVQMYVDMGGDDSGPYYRQQLAMESADP
                     MADDYYQMPFVDTESIEYLFMDQFLPGLRAELRPQFFLTPRLALDLRLGFMWMRPLMV
                     REVHAAHASWWFYQEGKDALAWSPTEGYAPGSVYDELYSGEERASWVYYGWNPLLRPD
                     GERWAFDISCLYAYVGFSMFF"
     gene            2079..>3079
                     /gene="mtnA"
                     /locus_tag="PLQ29_08525"
     CDS             2079..>3079
                     /gene="mtnA"
                     /locus_tag="PLQ29_08525"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPG86731.1"
                     /translation="MSQIIAMEYAEDRLRLVDQRRLPGSYEFFECRTYRDCAFAIKDM
                     VVRGAPAIGAAAAYGAALAASEFSSLGAAEFGPAMRVALDELRRARPTAVNLSWAVDR
                     MACALDKAIAGGTGPGRTLYERLKAEADAILADETARSERLSEHGNALIPDGAAILTH
                     CNTGSLAVPGPGTALGMIKAAHRAGKGVSVYADETRPRLQGARLTAWELDREGVPVTL
                     IADSVAATLMRDGKIGLVVVGADRIAANGDAANKIGTFMLSVLAARYGVPFYVAAPVS
                     TIDFACPSGDAIEIEERPADEVTTIEGVRVAPDGIHVYNPAFDVTPAEHITAIVTERG
                     "
BASE COUNT          510 a          995 c          977 g          597 t
ORIGIN      
        1 actggtacac tgatggaacc tgaggtagtg gggatgtctt tgagtgtttt caagatggat
       61 gtccctttta atacctcgat ctttaccgtt ggtccggggc ttccggtaga actccagctg
      121 ataacctgcg tggatccctg cgtccatatc tcaccaccat ttggtgattc taccttgatt
      181 gatgcgccac ttgcagtgtt gatggtaaag ggtgcatcac tgatatcggt gtacgctgca
      241 ttgctggcta gtatcgtatc atccgtactg ctcatgtccg tcttcctgcc cgtcattggt
      301 acctggatag gtagccgatc gttcagcgtg gaaccgatca ggatgttctt ccataagtgg
      361 ccgcgcaact tcgctctatc gctcggcgtc gaggctctcg tcgcccagcc gatcgctcga
      421 caggtcttgc tgcgtctgca cctgcgcaag gacgcccgtg cttcgcttta gggcaataga
      481 ctattctttc gtcgtatttg aacgccccgg gccggcggct tatagtatga gcgtcttcgt
      541 ctcaccgtcc gctatagagc gccgaggagg gaatcatgaa gaggctcgcc cttgtccttg
      601 tagctatctg cgccttgccg ctcggggccg acgagctgga ggacgccctg taccgcagcg
      661 tcgacgcgct ttcccaggct tataggaagg cgcatccgga cctcggcatg agggccggcc
      721 ttgccatgct cgatttcgag gagcagtctg agctcgccag gcgccacggc ctcggtacgg
      781 cgctcgagag ctatgtgcgc gacacggtcg agcgctccct ggtctacgag ctcgtcgacc
      841 gcagacggat gtccgatatc ctcgcggagc tggagctgag cctctccggc ctggtcgacg
      901 gccgcgacgc cgtcgaggct ggccggatag ccggcgtacg cgccttcctc tggggcggca
      961 tcgccgagct gggcgactcg ttcgtcatca gcctcggggt gaccgacgcg gagaccggcc
     1021 tggtcctggc gaccgaatcc ttcgaacttc cgaagcgcct gcttgtctcc gcggccgagg
     1081 agctcgcgta ctcgtacgtc gcgccgaacg gcatcggctt gtccgtccat ggcttcgcgc
     1141 cgctgttcca gctgtcggac ctctatacca aatcgtcgct cttcttcgcc gatctcggct
     1201 tttcctaccg gccgaaccgc cacttcatgg tgtcgggcgg catcatgacc gccccctcgc
     1261 tgaccgggga gcactacagg gtcgacgagg atatcgacgt gctgtccgtc cagccggagc
     1321 tggccgcgac ggtagaccca tggggattgg ccggctccac cgccaatgcc ggcttcagcc
     1381 aatggaccgc ccggttcagg gccaccttca tcaggctcga cgcccaatac acggtcaact
     1441 tcagcccacg cttcaatatt ggtttggccg gcggaatctt caacgcgctc ggcaatgtac
     1501 agatgtacgt cgatatgggc ggcgacgact cggggccgta ctacagacag caactggcca
     1561 tggaatccgc cgatccgatg gccgacgact actaccaaat gcccttcgtc gatacggaga
     1621 gcatcgagta cctgttcatg gaccagttcc tgcccggcct gcgcgccgag ctacggccgc
     1681 agttcttcct gacgccgcgc ctggcgctcg acctgcgcct ggggtttatg tggatgaggc
     1741 ccctgatggt cagggaggtg cacgccgccc acgccagctg gtggttctac caggagggca
     1801 aggatgcgct agcctggtcg ccgaccgagg gctacgcacc gggaagcgtc tatgacgagc
     1861 tatactcagg cgaggagcgg gcgagctggg tttattatgg ttggaacccg ctcctgcgac
     1921 cggatggcga gcgctgggcc ttcgatatca gctgccttta cgcgtacgtg gggttttcga
     1981 tgtttttcta aaaccccggc ctcgccgcgt ttgacagagc cccccttggg tactactatc
     2041 ggcatactag ctaaacccga ggggagcgaa cccgattcat gagccagatc atagccatgg
     2101 aatatgccga ggacaggttg cgcctcgtgg accagcgccg cctgcccggc tcgtacgagt
     2161 tcttcgagtg ccggacctac cgcgactgcg cgttcgcgat caaggacatg gtcgtccgcg
     2221 gcgcgccagc gatcggggcc gccgctgcct acggggcggc gctcgccgcg tcggagttct
     2281 cgtcgctcgg ggccgccgag ttcggcccgg ccatgcgcgt ggctctcgac gagctgaggc
     2341 gggccaggcc gacagcggtc aacctgagct gggccgtgga tcgcatggcc tgcgccctcg
     2401 acaaggcgat cgcgggcggg acagggccgg gccggacgct ctacgagcgg ctcaaagccg
     2461 aggcggacgc catcctggcc gacgagacgg cccgtagcga gcgcctatcg gaacatggca
     2521 acgccctcat ccccgatggt gcggcgatac tgacccactg caatacgggt tcgctggccg
     2581 tgccggggcc gggcaccgcg ctcggtatga tcaaagcggc ccaccgggcc ggcaagggcg
     2641 tatcggtcta cgccgacgag acgcgtccgc gcctccaggg cgctcgcttg accgcctggg
     2701 aactcgaccg cgagggcgtg ccggtcaccc tgatagccga cagcgtcgcc gcgacactga
     2761 tgcgggacgg gaagatcggc ctggtcgtcg tcggggccga ccgtatcgcc gccaacggtg
     2821 acgcggcgaa caagataggc accttcatgc tgtcggtcct ggccgcgcgc tacggcgtgc
     2881 ccttctacgt ggccgcgccc gtatcgacga tagacttcgc ttgcccatcc ggcgacgcga
     2941 tcgagatcga ggagcgccca gccgacgagg tgacgactat cgagggcgtc agggtcgcgc
     3001 cggacggcat ccacgtctat aacccggcct tcgacgtgac gccggccgag catatcacgg
     3061 ccatcgtcac cgaacgcgg
//
LOCUS       DAOPHV010000310         3076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_44152_length_3076_cov_4.431976, whole genome shotgun sequence.
ACCESSION   DAOPHV010000310 DAOPHV010000000
VERSION     DAOPHV010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44152_length_3076_cov_4.431976"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLQ29_08530"
     CDS             complement(<1..203)
                     /locus_tag="PLQ29_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPG86732.1"
                     /translation="MDLGLSGRTALVAASTDGLGLATARVLVAEGANVWIGGRDPARL
                     AAALESLRAPERDGRRVGRVEGAV"
     gene            384..884
                     /locus_tag="PLQ29_08535"
     CDS             384..884
                     /locus_tag="PLQ29_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86733.1"
                     /translation="MHTKQTETVMIVGLLILALSAPAYAQGGFKEGKALSLGSEGGVF
                     SKPTYAQEGRLVAAGAVDAFLLYDLEETRTVMLEVARLDTAHVATSIGGTALMIGGLA
                     TFFVAASGVSDNRGTISIYLTSAGLFLGSAGAWAGNWHCQKQRVRLRLEAIELYNRRY
                     GLVAEP"
     gene            complement(912..1736)
                     /locus_tag="PLQ29_08540"
     CDS             complement(912..1736)
                     /locus_tag="PLQ29_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86734.1"
                     /translation="MAKERAGTTGRGKASGKADSAQLRKDPAFMRNLESAIAEEYARS
                     RRKALPAASSPDEATPRDLGPTKGAAPDGFGAAPKRATEASAPNTPRRAPKKAGSAKG
                     GTGREALAKELASLIPELDAEGLAFLIEQARVHLYNMRVAELEAAAEEAERASTRARG
                     LAKTGGIGTKAGTKAGTKAGSASDLSIKASGDGSAYDLVYGGKWKLFTGDEMLAMVRI
                     ATVKDPQDQVAGRLYRWLLAERSDVINDLGLSGLADPMLKHLVALLRTTFTIKPGR"
     gene            1874..2071
                     /locus_tag="PLQ29_08545"
     CDS             1874..2071
                     /locus_tag="PLQ29_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPG86735.1"
                     /translation="MKNRIRERRQGLGLTQEQLAEQVGVSRQTIISIEGGRYDPSLPL
                     ALKLARLLGQSIEDLFIDQEE"
     gene            2073..2483
                     /locus_tag="PLQ29_08550"
     CDS             2073..2483
                     /locus_tag="PLQ29_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86736.1"
                     /translation="MGFFGNLTMRRYAATNLILGAMFINLLLFGGRLEMGEAFTAGLA
                     TGLGVGWLAFGIGGWIALKRPGRTWDERTATINMKASAISFCVVMLVTALMSAALRSR
                     TLGMDWSAADLAGMLVNLGLASFGLSALVIRKRT"
     gene            2551..>3076
                     /locus_tag="PLQ29_08555"
     CDS             2551..>3076
                     /locus_tag="PLQ29_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPG86737.1"
                     /translation="MDRCGLVDVATLGAGIIVDLRYATSDNFLGTRLYDGRSAWLLAP
                     AAAKLAVAAAHAATDGRSIVVLDAYRPLSAQALMWAARPEPGFVAPPERGSNHNRGAA
                     VDVTLADTDGRELPMPSAFDEFSERASHAYSGGPPDALANRDALRSYMERAGFVAYEA
                     EWWHYNDPESKSGPL"
BASE COUNT          500 a         1051 c          979 g          546 t
ORIGIN      
        1 acggcgcctt cgacccggcc gactcgtcgt ccgtcacgtt caggcgcgcg caaagactcg
       61 agcgcggccg ccagcctagc cgggtcacgc ccgcctatcc atacgttcgc gccctcggct
      121 accagcaccc tggccgtggc gagccccagc ccatcggtag acgccgcgac gagggccgtt
      181 ctgccgctca gtcctagatc catatccacc tcccagacta tcggatctaa agatacgccg
      241 tctgccaccg gcgatcaaat cggttgatgc ccgcgcggac cttgcgcccc cggacaggcg
      301 ggctatactt gatcgtatac ggtagaaagt atccgcatcg tgtcgttcac cgctaccata
      361 gatcgaagca gtggaggtaa gcgatgcaca cgaagcaaac ggagaccgtc atgatcgtcg
      421 gcctcttgat cctcgcgctc tccgccccgg cctacgctca aggcggcttc aaggaaggca
      481 aggcgctgag cctgggctcc gagggcggcg tcttctcgaa gccgacctac gctcaggaag
      541 gcaggctcgt cgcggccgga gccgtcgacg cgttcctgct ctacgacctc gaggagacga
      601 ggaccgtcat gctggaggtc gcgcgcctcg atacggccca cgtcgccacc tcgataggcg
      661 gcaccgccct gatgatagga ggcctggcca ccttcttcgt cgccgcgagc ggggtctcgg
      721 acaatcgcgg caccatcagc atctacctga cctcggccgg cctgttcctc ggcagcgccg
      781 gagcctgggc gggcaactgg cactgccaga aacagcgcgt acgcctgcga ctggaggcta
      841 tcgagctcta caaccggcgc tacggcctgg tagcggagcc ctgagatgcg ccgttcgatt
      901 ggaccggctc cctagcgacc gggcttgatc gtgaaggtcg tgcgaagtag cgcgacgaga
      961 tgcttgagca tcggatccgc caggcccgac aggcccaggt cgttgatcac gtcggatcgc
     1021 tcggccagga gccagcggta gagcctgccg gccacctggt cctgcgggtc cttgaccgtg
     1081 gcgatgcgga ccatcgcgag catctcgtcg ccggtgaaca gcttccactt gccgccatag
     1141 accaggtcgt aggccgagcc gtctcccgag gccttgatcg acaggtcgga ggcggagccg
     1201 gccttggttc cggccttggt tccggccttg gttccgatac cgccggtctt cgctaggccg
     1261 cgggcccggg tagacgcccg ctcggcctcc tcggccgccg cctcgagctc ggccacccgc
     1321 atattgtaca ggtggaccct cgcctgctcg atcaggaagg cgagcccctc ggcgtcgagt
     1381 tccggtataa gcgaggccag ttccttggcg agcgcttcac gcccggtccc gcccttggcg
     1441 ctcccggcct tcttcggagc gcgcctcggc gtattcggag cgctcgcctc ggttgctcgc
     1501 ttcggcgccg cgccgaaccc gtctggggcc gctccctttg ttggcccaag gtcacgcggc
     1561 gtagcttcgt ccgggctcga cgcggccggt agggcctttc gccgcgacct ggcgtactcc
     1621 tcggctatgg ccgactcgag attcctcatg aaggccggat ccttcctgag ctgggccgaa
     1681 tcggccttgc ccgaagcctt accccgaccc gtcgttccgg cgcgttcctt ggccatgatc
     1741 cctcctcgtc gcgacagcga ctactgtaca ccctggcgcc gcgattttca tccttgcagc
     1801 caggtaaagt ctgcttgaca ttcaatgcta tcatgagtat agtgaatgta aagctagctt
     1861 gacaggatac ggaatgaaga atcgcatacg agaacgcagg caaggactgg ggctgaccca
     1921 ggaacaactc gccgagcagg tcggcgtttc aaggcagacc atcatctcga tcgagggcgg
     1981 ccgctacgac ccgtcgctcc cgctcgcctt gaaactcgcc agactactcg ggcagagcat
     2041 cgaagacttg ttcattgacc aggaggaata agatgggatt tttcggaaac ctgaccatgc
     2101 gccgctatgc ggccaccaac ctgatcctcg gcgccatgtt catcaacctg ttgcttttcg
     2161 gcggccggct cgagatgggc gaggccttca ccgccggcct ggccaccggc ctcggcgtcg
     2221 gctggctggc cttcgggata ggcgggtgga tagccttgaa gcgccccgga cggacctggg
     2281 acgagcgcac ggcgaccatc aacatgaagg cgtcggcgat atcgttctgc gtcgtcatgc
     2341 tcgtaacggc gcttatgtct gcggcgctgc gttcccgcac cctcggcatg gactggagcg
     2401 cggccgacct ggcgggcatg ctcgtcaatc tcgggctggc gagcttcggc ctttcggctt
     2461 tggtcatccg gaaacgtaca tagacccgaa gcgggcctcg ggtggcgccg gagctgtaca
     2521 cggcggccgc ccgagggtag catcgtcgcc atggacagat gcggattggt cgacgtagcg
     2581 acgctgggag ccggcatcat cgtcgacctg cgctatgcga cgagcgataa cttcctgggg
     2641 acgcggctgt acgacggccg gagcgcctgg ctgctagcgc cggcggcggc caagctggcc
     2701 gtcgccgcgg cccatgccgc taccgacggg cgatccatcg tcgtgctcga tgcctaccgt
     2761 ccactatccg cccaggcgct catgtgggcc gcgcggccgg aacccggttt cgtagccccg
     2821 ccggagcgcg gttcgaacca caaccgcggc gccgccgtcg atgtgacgct ggccgacacg
     2881 gacggccgag agctacccat gccgagcgcc tttgacgagt tctccgagcg cgcgtcgcac
     2941 gcctactcgg ggggccctcc ggatgccctg gccaatcggg acgcgctacg ctcgtacatg
     3001 gagcgggccg gcttcgtcgc ctacgaggcc gagtggtggc attataacga tccggagtcg
     3061 aagtccggcc ccctgc
//
LOCUS       DAOPHV010000311         3073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_44225_length_3073_cov_3.108350, whole genome shotgun sequence.
ACCESSION   DAOPHV010000311 DAOPHV010000000
VERSION     DAOPHV010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44225_length_3073_cov_3.108350"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1162
                     /locus_tag="PLQ29_08560"
     CDS             167..1162
                     /locus_tag="PLQ29_08560"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPG86738.1"
                     /translation="MTIDEAAAFLSGRDDFILSSHEGPDADGLGAEYALARALSSLGK
                     RVRVVNANRHSETYRFIDRAGIIECIDDAGLDDEEIRASTCVLLDTNDIMYAGEVADR
                     IIAKAKGLIIIDHHEVKGDAAPNVCSAPSYSSTCEMLYLILKRLGHVFTEETASALFA
                     GMVYDTGSFAYSKTTADTFEAALELVRAGADPTKIHGALYESSAVNVLLLRKDVLATL
                     ELYGGNKIAVQTLTSGMLAETGSSSQDAEGLINVPLQAADVEVSIFLKENEEGLLRCS
                     LRSKGAINVAQIAQSFGGGGHRSAAGFKSPYPLDTIKGRVLELVVIALAQRQDSD"
     gene            1201..2712
                     /locus_tag="PLQ29_08565"
     CDS             1201..2712
                     /locus_tag="PLQ29_08565"
                     /inference="COORDINATES: protein motif:HMM:NF033751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pallilysin-related adhesin"
                     /protein_id="HPG86739.1"
                     /translation="MKRITHTAFAIAVVATLAVATYFFLSERDGRKADEPVRYRALEA
                     VDATNPSERPIEAAQAIRAAASIPLEPNETLLDLYSFNLDYDDEEEQVLVVRDSDDAT
                     GVVKIVLADYSPFTRKWTRSWKGSTLVTKNKTFQVSVNDLIGDHNLNIVCTGMDDANN
                     QTMTVYWKSQDAYRDQQAAYIKILEQAGSAVLVESRDRPDSYKLGQSNAESWPVSVWK
                     EDAGSGNYLDQIKETWQWSFADEAYVLAGTVSIPGASIARQKAESILDGTAETFKAFL
                     DGTWYKESSDPLSSGALFITFQSREEAVLFSGEGLTEVYTWENSNPTRYGLYIASRNQ
                     SVRNLRRLMDIELASADSVNVRVFQDYRIKADISGRWDGRYRRLTPNLAAAFRRSPAM
                     ARSAQSDIGGLYESDDGSSLSLVGSEYVLKSPDGEQRGAFSIYELQGRVVLDTRRIAD
                     GDGESVRRSWVATVAESRGPDGQSVQTLTLEEATIGILGIEPIQSPPLSFTRR"
     gene            complement(2724..>3073)
                     /locus_tag="PLQ29_08570"
     CDS             complement(2724..>3073)
                     /locus_tag="PLQ29_08570"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPG86740.1"
                     /translation="RPEADSSAIAALSLASRSVIKEPAELSEPAVAADPVQPVAADGA
                     RKASYQVGAFRERANADALVSRLGGLGVTAKVARKADSGLFVVVAEGGVDPARTVLIL
                     KDAGFEAWAVEGP"
BASE COUNT          613 a         1009 c          958 g          493 t
ORIGIN      
        1 cgtcgcgcgc tgccgacggt tccggcaagg cctccttcgc cggcgcgccc ttggccgcga
       61 gcctggcggc ggctggagcc ataggcttgg tcgtggccat cgtcgcgttt aggcgtcggc
      121 gtcgcaggga taggggcgct gggaacgagc cggaggaacc taccgcatga cgatcgacga
      181 ggccgcggcg ttcctgtccg gccgggacga ttttatcctt tcgtcgcacg agggccccga
      241 cgcggacggg ctgggagccg agtacgccct cgccagagcc ctttcgtcgc tcggcaaacg
      301 cgtgcgcgtc gtcaacgcca acaggcactc cgaaacctac aggttcatag acagggccgg
      361 catcatcgag tgcatcgacg acgccgggct cgacgacgag gagatacgag cctcgacctg
      421 cgtgctactc gacacgaacg acataatgta cgcgggcgag gtggccgatc gcatcatcgc
      481 caaggccaag ggattgataa tcatcgacca ccacgaggta aagggcgacg cggccccgaa
      541 cgtctgctcc gcgccgagct actcgtcgac ctgcgagatg ctctacctga tactaaagcg
      601 actcgggcac gtgttcaccg aggaaacggc ctcggccctg ttcgccggca tggtctacga
      661 caccggctcc ttcgcctact ccaagacgac ggccgacacc ttcgaggccg cgctggagct
      721 ggtccgcgcc ggagccgatc cgacgaagat ccacggcgcg ctctacgagt cgtcggccgt
      781 gaacgtgctc ctgctacgca aggacgtgct cgccaccctc gagctatacg gcggcaacaa
      841 gatcgccgta cagacgctta cctccggcat gctcgccgag acgggttcat cgagccaaga
      901 cgccgagggc ctcatcaacg taccgctcca ggcggccgac gtcgaggtct cgatcttcct
      961 caaggagaac gaggagggtc tgctcaggtg ctccctcagg tccaaagggg cgatcaacgt
     1021 agcccagata gcccagtcct tcggcggagg cggtcatcgg agcgccgccg gcttcaagtc
     1081 gccctatccc cttgatacga tcaagggccg agtgctagaa ctcgtcgtca tagcgctggc
     1141 tcagcggcag gattccgact agggttcaga gacggccgca aacaaggaac gatacgccgc
     1201 atgaaacgta taacccatac agcattcgcc atagccgtcg tcgcgacgct cgcggtcgca
     1261 acctatttct tcctctccga gcgagacgga cgcaaggccg acgaaccggt ccgctatcgg
     1321 gccctcgaag ccgtagacgc caccaatcca tcggagcgcc cgatcgaggc ggcccaggcg
     1381 atccgggccg ccgcgtctat accgctcgag ccgaacgaaa ccctgcttga cctgtactcg
     1441 ttcaacctcg actacgacga tgaggaggag caggttctgg tcgtgcgcga ctccgacgac
     1501 gcgaccggag tcgtcaagat agtgctagcc gactactcgc cgttcacgcg caagtggacg
     1561 cgctcatgga agggctcgac gctcgtcacc aagaataaga ccttccaggt gagcgtgaac
     1621 gacctcattg gcgaccacaa cctgaacatc gtatgcacgg gcatggatga cgccaataac
     1681 cagaccatga cggtgtattg gaagtcgcag gatgcgtatc gagaccagca agccgcctac
     1741 atcaagatcc tcgagcaggc aggatcggcc gtactcgtcg agagccgcga taggccggac
     1801 agctataagc tcggccagtc gaacgccgag agctggcccg tctccgtctg gaaggaggat
     1861 gcgggctcgg gtaattacct cgaccagatc aaggaaacct ggcaatggag cttcgccgac
     1921 gaagcctacg tgctggccgg tacggtgagc atccccggcg ccagcatcgc tcggcagaag
     1981 gctgaatcga tcctcgacgg cacggccgag accttcaagg ccttcctcga cggcacctgg
     2041 tacaaggaat cgagcgaccc gttgtcctcg ggggccttgt tcatcacctt ccagtcaagg
     2101 gaagaggcgg tgcttttctc cggcgaaggg ctgaccgaag tatacacctg ggagaactcc
     2161 aacccaacgc gctatggcct ctacatagcc tcgcggaacc agtcggtcag gaatctgcgc
     2221 cggctcatgg acatcgagct cgcctctgca gactcggtca acgtcagggt cttccaggat
     2281 taccgcatca aggccgacat atccggaaga tgggacggca ggtaccgcag gttgacgccc
     2341 aacctcgccg cggccttcag gcgctcgccg gccatggcca ggagcgccca atccgatata
     2401 ggcgggctct acgagtcgga cgacggctcg tcgctgtcgc tggtcggctc ggaatacgtg
     2461 ctgaaaagcc ctgacggaga gcagcgcggc gcattctcaa tctatgagct acagggccga
     2521 gtcgtcctcg acacgaggcg tatcgccgac ggggacgggg agagtgtcag gcgctcgtgg
     2581 gtcgcgaccg tggcggagtc gcgcggcccc gacggccaat cggtacagac actgacgctg
     2641 gaggaagcga caataggcat cctcgggatc gagccgatcc agtcgccgcc gctgtcgttc
     2701 acgcggcgct agtggccttc gggctagggg ccttctacag cccaggcctc gaagccggca
     2761 tccttgagga taagcaccgt cctcgccgga tcgaccccgc cctcggctac gactacgaac
     2821 agccctgaat cggccttacg cgccaccttc gccgttacgc cgagcccccc gagcctcgaa
     2881 acgagagcgt cggcgttggc ccgttccctg aacgctccta cctggtagga ggccttccga
     2941 gcgccgtctg ccgcgaccgg ctgaaccgga tctgccgcta ccgccggctc agagagttcg
     3001 gcaggctcct tgatgaccga ccgcgaggcg agggacagcg ctgcgatagc cgaggagtcg
     3061 gcctcgggac gcg
//
LOCUS       DAOPHV010000312         3047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_44747_length_3047_cov_3.215575, whole genome shotgun sequence.
ACCESSION   DAOPHV010000312 DAOPHV010000000
VERSION     DAOPHV010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44747_length_3047_cov_3.215575"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLQ29_08575"
     CDS             complement(<1..301)
                     /locus_tag="PLQ29_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HPG86741.1"
                     /translation="MSRFIVRRFLSVIPTLFIIVTLSFFLIRLAPGGPFSGEKKLPPE
                     IMANIMRKYHMDEPLVKQYFRYMGDVLQFDLGPSYRYKDQTVNDLIGNAFPVSVML"
     gene            complement(544..2124)
                     /locus_tag="PLQ29_08580"
     CDS             complement(544..2124)
                     /locus_tag="PLQ29_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HPG86742.1"
                     /translation="MKRVLSGLLLIVMAATLIVSCGGKGPAKGVEFVMNNGAEPQTLD
                     PSKIQGVPEHKIYMALFEGLVGYDPKTSLATPGVAESWTVSPDGTQVTFKLRKTTWSD
                     GTKITAQTVVDSWLRTLDPATASEYAYMAGMVVKGANEFNAGEAGKEAVQIRALDDYT
                     FQVDLLGPMPYAVDMMAHYTFAILPMHVINAKGEDWIKVENIVGNGPFKLAEWKPQES
                     LTVVPNDKYWDAKTVKLSKITFLPIDDNLTAYNKFKAGEVDWAHGIPLSLVDEIKLRP
                     DYQVAPQVATYYYLFNVTRKPFDDVRVRKALTMALDMQELVDKVTKGGQLATSSMVPA
                     MEGYTPAKGPTFNVEEARKLLAEAGFPDGKGFPKTPILYNTSDGHKIIAEWVQESWKK
                     NLGIEVTLLNKEWKTFLDTRSNAHDFDIARAGWVGDYLDPNTFLDMFIIGSGNNDGLY
                     ANPEYDALVKKAGTMKAGPERMATLQAAEALLIAKDQAVIPFYHYVEQDLIDTTKWDG
                     WYANPLGVHDWKYIGPKK"
     gene            complement(2321..2764)
                     /locus_tag="PLQ29_08585"
     CDS             complement(2321..2764)
                     /locus_tag="PLQ29_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPG86743.1"
                     /translation="MKSVITYNPASVLSALDDWDKLVGDFFGRDYQYSAGTRGYPAVD
                     ISEEKERYLLEAELPGFAEKDVDIKVNDRVLTIASSKEEEKRSERDGVWLIRERGVRT
                     FRRSFSMPRDVDLDGIKAVFKDGLLSVSLPKRPEAREKTVEISRS"
BASE COUNT          581 a          857 c          962 g          647 t
ORIGIN      
        1 cgagcatcac ggagaccggg aaggcattgc ctatcaggtc gttgacggtc tgatccttgt
       61 atcgatagga cggaccgagg tcgaactgca gtacatcgcc catatagcgg aagtactgtt
      121 taacaagcgg ctcgtccatg tggtatttcc gcatgatgtt cgccatgatt tccggcggca
      181 gttttttctc gccggagaaa ggcccgccgg gggcgagcct gatcaggaag aagctgagcg
      241 tcacgatgat gaacagggtc ggtatgacgc tcaggaatct tcggacgatg aatctagaca
      301 tcgttgcctc gcttgaaaag ggtacaggcc attcgaggat agtcgaacga cctaccgcgg
      361 cgcccctatc cgctaggacg gctacgccgc gagaaagcca gtatacaccc atgtaccggg
      421 atttgccaga aatttcttag ctaatcgtaa ttatttgaaa tgtcgttttg ttatttgaaa
      481 gtaatagcta catcggctaa aaaaggcccg gcgccaaagc gccgggcccg ttggctcaaa
      541 gccttacttc ttggggccga tgtacttcca gtcgtggacg ccgagcgggt tggcgtacca
      601 gccgtcccac ttggtcgtgt cgatcagatc ctgctccacg tagtggtaga acgggatgac
      661 cgcctgatcc ttggcgatca gcagagcctc ggccgcctgg agggtggcca tgcgctcggg
      721 accggccttc atcgtgccgg ccttcttcac gagggcgtcg tactcggggt tcgcgtacag
      781 gccgtcgttg ttgccggagc cgatgatgaa catgtcgagg aaggtgttcg ggtcgaggta
      841 gtcgcccacc cagccggcgc gcgcgatgtc gaaatcgtgg gcgttcgagc gggtgtcgag
      901 gaaggtcttc cactccttgt tgagcagggt aacctcgatg ccgagattct tcttccagga
      961 ctcctgcacc cactcggcga tgatcttatg accgtcgctg gtgttgtaca ggatcggggt
     1021 cttggggaag cccttgccgt ccgggaagcc ggcctcggcg aggagcttgc gggcctcttc
     1081 cacgttgaag gtcggaccct tggcgggcgt gtagccttcc atggccggga ccatcgagct
     1141 ggtggccagc tggccgccct tggtcacctt gtcgacgagc tcctgcatgt cgagggccat
     1201 cgtcagcgcc ttgcggacgc ggacgtcgtc gaacggcttg cgggtcacgt tgaacagata
     1261 atagtaggta gcgacctgcg gggcgacctg gtagtccggg cgaagcttga tctcgtcgac
     1321 caggctgagc gggataccgt gggcccagtc gacctcgccg gccttgaact tgttataggc
     1381 ggtgaggttg tcgtcgatcg ggaggaaggt gatcttggag agcttgacgg tcttggcgtc
     1441 ccagtactta tcgttcggga cgaccgtgag ggactcctgg ggcttccact cggcgagctt
     1501 gaaggggccg ttgccgacga tgttctcgac cttgatccag tcctcgccct tggcgttgat
     1561 gacgtgcatc gggaggatgg cgaacgtgta gtgagccatc atgtccacgg cgtagggcat
     1621 cgggccgagc aggtcgacct ggaaggtgta gtcgtcgagc gcgcggatct gcacggcttc
     1681 cttgccggcc tcgcccgcgt tgaactcgtt ggcgcccttg acgaccatgc cggccatgta
     1741 cgcgtactcg gacgcggtgg cggggtcgag ggtgcgcagc cagctgtcga ccacggtctg
     1801 ggccgtgatc ttggtgccgt cggaccaggt ggtcttgcgg agcttgaagg tgacctgggt
     1861 gccgtcgggg ctgacggtcc agctctcggc tacgccgggc gtggcgagcg aggtcttggg
     1921 atcgtacccg acaaggccct cgaacaaggc catgtaaatc ttatgctcgg gaacgccctg
     1981 gatcttcgag ggatcgagcg tctgaggctc ggcgccgtta ttcattacga actcgacgcc
     2041 tttggccggg ccctttccac cgcaggagac gatcagggtc gccgccataa cgatgaggag
     2101 caggcctgag agaacacgtt tcatggttcc tccttgtatg gaacttgtgg ggctattgta
     2161 tgccccagaa atcaagaacg caagcatgtt tttacatggc tagacacgga acggccagaa
     2221 accgcgtaaa accgcatgag attactatac aaaagaacgg gccatcgtgt cgatggcccg
     2281 ttcggggcgc cgcggagcgg cgttcgaggc tagtcggcgc ttacgagcgc gaaatctcga
     2341 ccgtcttctc ccgcgcctcg gggcgcttgg gcagggagac cgacaggagg ccgtccttga
     2401 acacggcctt gatgccgtcc aggtcgacgt cgcgcggcat gctgaacgaa cggcggaagg
     2461 tcctgacgcc gcgctcgcgg atcagccata cgccgtcccg ctcggaccgc ttctcctcct
     2521 ccttggacga ggcgatggtc agcacgcggt cgttcacctt gatgtccacg tccttctcgg
     2581 cgaagcccgg cagctcggcc tcgagcaggt agcgctcctt ctcctcgcta atgtcgacgg
     2641 cgggatagcc cctggtgccg gcgctgtact ggtagtcgcg gccgaagaag tccccgacca
     2701 gcttgtccca gtcgtcgagg gccgagagca cggaagccgg attgtaggtg atgacgctct
     2761 tcatgataaa cctcctaaaa ggtgttgtat aaaacattgg cgcggagggg cgacctacgg
     2821 tcgatcaccg cgtccaccca ctataaagca agaaacgtgc caaataagcg ttacgccaag
     2881 caaaattttg acaataatca ccgctcggct ctatttgctt gttttgaata tacttagcta
     2941 ctaacatagt tcgatatcag ctcacacgat cgccatacgc cgatgacaac gccgaggctt
     3001 tctcgaagaa cccggcgtgg agcgaaacaa ttcgtttctt tttatcc
//
LOCUS       DAOPHV010000313         3040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_44891_length_3040_cov_3.556784, whole genome shotgun sequence.
ACCESSION   DAOPHV010000313 DAOPHV010000000
VERSION     DAOPHV010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44891_length_3040_cov_3.556784"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /gene="tsaE"
                     /locus_tag="PLQ29_08590"
     CDS             <1..468
                     /gene="tsaE"
                     /locus_tag="PLQ29_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPG86744.1"
                     /translation="SRPGQARGPTLIRLECPGVEATERLGERIGQAAPPGPVIAFRGG
                     LGAGKTAMCRGIARGLGVTAPVTSPTYTLINEYEGERPFYHFDAYRLSSADEFDQLDA
                     KRYFYGDGVCAIEWSERVAAAMPPDAVVIAIEPSADGSRVVTVSGPALEGALA"
     gene            465..1238
                     /gene="tsaB"
                     /locus_tag="PLQ29_08595"
     CDS             465..1238
                     /gene="tsaB"
                     /locus_tag="PLQ29_08595"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPG86745.1"
                     /translation="MNILAVDCSGDILSVGAGRSMGIAGRGPEAPKHPYREERGHGHA
                     AGPSFACVSVDAGFRHAERVMSAVEYCLGEAGLAPSELDLLACAGGPGSFTGLRIGMA
                     TVKGLSLGLDKPFVAVPTLDAMAADWEGASRIIVPIIDAKRARFYFAIYDGGRLVAGP
                     FDDGLPRLIELTQAYPELLFVGPDADLLESSAAERPGFRIAAERRRSPIAAMARLAVG
                     IYERDGPAPSDAGLEYLRASDAEEAAAATSGAAANGAAS"
     gene            1235..2341
                     /locus_tag="PLQ29_08600"
     CDS             1235..2341
                     /locus_tag="PLQ29_08600"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPG86746.1"
                     /translation="MKPRDEPELLEELEPAESLDQSEPAQPIELEASGPTDAAALQRI
                     VKDLVSSYSLVPIPVAILDGTMSFLYTNEPFKRLLESFHASGKPTLIGAIGRFLDQAT
                     ARDLYLAIKDPRRGYSWHGQIRLKAKDSSSVIAKTTIMPFRPDASSDGRPMAWVAFLD
                     DVTEERNALIRGLFSSLLEASKLKDNDTGRHIERVNLYAERLASALYESGRWPEVDVD
                     FVENIGFLAAMHDVGKIGTPDDILNKKGPLDEFEWNIMKEHTINGAFILSSYPNPMAK
                     QIAQSHHEWWNGTGYPYNLVGAMIPLPARIVALADVYDALRMRRSYKLPFDHARASLL
                     IVADGGKHFDPDVVEVFKRIGHDFDTIFNDNIDA"
     gene            complement(2347..2598)
                     /locus_tag="PLQ29_08605"
     CDS             complement(2347..2598)
                     /locus_tag="PLQ29_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86747.1"
                     /translation="MSSIEWKDKAYDDAYSETLASLARKRTEDPAFSAEDARGVLKHL
                     YIQDGNDWGGRGELQDITIRATIDAYERFIAEWVSDKAP"
     gene            complement(2661..2733)
                     /locus_tag="PLQ29_08610"
     tRNA            complement(2661..2733)
                     /locus_tag="PLQ29_08610"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2698..2700),aa:Met,seq:cat)
     gene            complement(2812..>3040)
                     /locus_tag="PLQ29_08615"
     CDS             complement(2812..>3040)
                     /locus_tag="PLQ29_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86748.1"
                     /translation="AQSLQSGIVLGYGGLVSRLVELMSAEMGGSALVIGTGDETGRTV
                     LESAGYDRFVPNLALDGLSILAERDRISAKR"
BASE COUNT          522 a         1045 c          984 g          489 t
ORIGIN      
        1 agccgacctg gacaagcgcg gggtcctacc ctgatacgcc tcgagtgccc cggcgtcgag
       61 gcaaccgagc gcctcggcga gcgcataggc caggcggctc ctcccggtcc cgtcatcgcg
      121 ttccggggcg ggcttggggc gggcaagacc gccatgtgcc gcggcatagc ccgtggtctc
      181 ggcgtcaccg ctccggtgac cagcccgacc tacaccttga taaacgaata cgaaggcgag
      241 cggcccttct accatttcga cgcctaccgc ctgtcgtcgg ccgacgagtt cgaccagctc
      301 gacgccaagc gctacttcta cggcgacggc gtctgcgcca tagaatggag cgagcgggtc
      361 gccgcggcca tgcctcccga cgccgtcgtg atcgctatcg agccgtcggc cgacggctcc
      421 cgcgtcgtga ccgtcagcgg ccccgcgctg gaaggggcct tggcgtgaac atcctggcgg
      481 tggactgctc gggcgacatc ctgtcggtcg gagccgggcg atcgatgggg atagccgggc
      541 gcggtcccga ggctcccaag catccgtacc gcgaggaacg cggtcatggc cacgccgccg
      601 gcccgagctt cgcgtgcgtc tcagtggacg ccgggttcag gcacgccgag cgcgtgatga
      661 gcgccgtcga gtattgcctc ggcgaggccg ggctcgcgcc ctcggagctc gacctgctcg
      721 cctgcgccgg cggaccgggc tccttcaccg gactgcgcat cggcatggcg acggtcaagg
      781 gcctgtcgct cgggctcgat aagcccttcg tcgcggtacc gaccctggac gccatggccg
      841 ccgactggga gggcgcgtcg aggataatcg taccgatcat cgacgccaag cgcgcacgct
      901 tctacttcgc catctacgac ggagggcgcc tcgtcgccgg ccccttcgac gacggcctcc
      961 cccgactcat cgagctgacc caggcctatc cggagctgct gttcgtcggc ccggacgccg
     1021 acctgctcga gtcgagcgcc gcggagcggc ccggcttcag gatcgcggcc gagcgcaggc
     1081 ggtcgcctat cgccgcgatg gccaggctgg ccgtcggcat ctacgagcgg gacggcccgg
     1141 ccccgtccga cgcggggctc gagtacctgc gcgccagcga cgccgaggag gccgccgcgg
     1201 cgacgtccgg cgcggcagcg aacggagccg catcatgaag ccccgggacg agccggagct
     1261 gctcgaggag ctggagccgg ccgaatcgct cgaccagtcc gaacccgccc agccgataga
     1321 actcgaggcg tccgggccga ccgacgccgc cgcgctccag cgcatcgtca aggacctcgt
     1381 ctcatcgtac tcgctcgtcc ctattcccgt cgcgatcctc gacggcacta tgtcgttcct
     1441 atatacgaac gaaccgttca agcggctgct cgaatcgttc cacgcgagcg gcaagcctac
     1501 cctgatagga gcgatcgggc gcttcctgga tcaggcgact gcgagggatc tctatctggc
     1561 gatcaaggac ccgaggcgcg gctacagctg gcatggccag atccggctca aggccaagga
     1621 ctcgagcagc gtgatcgcca agacgacgat catgcccttc aggcccgacg cgtcgagcga
     1681 tgggcggccg atggcctggg tagcctttct cgacgacgtg accgaggaac gcaacgctct
     1741 catccgaggg ctcttctcca gcctgctcga ggcgtcgaag cttaaggaca acgacaccgg
     1801 gcgccatatc gagcgggtca acctctacgc cgagcggctg gcgagtgccc tgtacgaatc
     1861 cgggcggtgg cccgaggtcg acgtggactt cgtggagaat atcggcttcc tcgcggccat
     1921 gcacgacgtg ggcaagatag gcacgccgga tgacatcctg aacaagaagg gtccgctgga
     1981 cgaattcgag tggaacatca tgaaggagca tacgatcaac ggcgccttca tcctgtcgag
     2041 ctacccgaac cccatggcca agcagatcgc ccagtcccac catgagtggt ggaacggcac
     2101 cggatatccg tataatctgg taggcgcgat gataccgcta ccggcccgca tcgtcgcgtt
     2161 ggcggacgtc tacgacgcgc tcaggatgag gagaagctac aagctgccct tcgaccacgc
     2221 gcgcgcctcg ctcctgatcg tcgccgacgg aggcaagcac ttcgatcccg acgtcgtcga
     2281 ggtgttcaag cgcatcggcc acgacttcga cacgatcttc aacgacaata tcgacgcgta
     2341 gggacgctag ggcgccttat ccgataccca ctcggcgatg aagcgctcgt aggcgtctat
     2401 cgtcgcccgt atcgttatat cctgaagctc gccgcggccg ccccagtcgt tgccgtcctg
     2461 gatatagagg tgcttgagca cgcccctcgc atcctccgcc gagaaggccg gatcctcggt
     2521 ccgcttacgc gctagactgg cgagcgtctc ggagtacgcg tcgtcatagg ccttgtcctt
     2581 ccattctatc gatgacacga tggctcctac ggggtacata gacgaagagg ccgccccgag
     2641 gggcggcctc tcctacaatg tagcggcggg ggggattgaa ccctcgacac aacggatatg
     2701 agccgtttgc tctaccgact gagctacgcc gccattcgtg acgcccgcac tttatacaga
     2761 acggcgcgcc ttttgtcaac agccgccgcc tggccgagtg tcgacggcgg ctcagcgctt
     2821 ggcgcttatc cggtcgcgct cggccaggat gctgagtccg tcgagggcca ggttcggcac
     2881 gaagcgatca tagccggcgc tctcgagcac cgtccgcccg gtttcgtcgc cggtgcctat
     2941 caccagggcg gagccgccca tctcggcgga catcagctcg accaggcggc tgacgagacc
     3001 gccgtagccg agcacgatgc cggactggag cgactgggcg
//
LOCUS       DAOPHV010000314         3039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_44913_length_3039_cov_4.152480, whole genome shotgun sequence.
ACCESSION   DAOPHV010000314 DAOPHV010000000
VERSION     DAOPHV010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44913_length_3039_cov_4.152480"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..855)
                     /locus_tag="PLQ29_08620"
     CDS             complement(226..855)
                     /locus_tag="PLQ29_08620"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPG86749.1"
                     /translation="MILGLTGGYCAGKSSAAAALHEAGWTVIDVDALGHAALERSAPE
                     VARLLGPGALKADGAPDRRAIGAMVFADPVLLARYEAIVHPAMNALVEEAVSRAGPRA
                     CVDAAILYRLPIVAACDTIIEARAPLALRLIRGRARDGLGALAMLGRIRSQRPLRDAG
                     KAWKGKTVVVWNALGEDGFKRRVVGLAERAFRSLAADLGAVDREPAPGA"
     gene            complement(852..>3039)
                     /gene="polA"
                     /locus_tag="PLQ29_08625"
     CDS             complement(852..>3039)
                     /gene="polA"
                     /locus_tag="PLQ29_08625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPG86750.1"
                     /translation="AEVKPDGLRRKLEAGRDSAMLSKELITLAYDAPLPVASLDELAV
                     PSIDRDAASSVFMREGMRSLATRRVADGLDKLEAAGELFAAAGSQAGTQAGTQAGTHE
                     APATRADKTIAETIDEADVSPTAARGLPESYGRPGTHELVLDEAGLRRWVDAAERAGV
                     FAFDCETDSLDEMRVMPVGFSIAVSPDAACYVPLAAPDAACLSAETVRRLVAPLLARA
                     DLVVVGQNLKFDMHIMENWGAPIHCAPWDTMIAAWLVDPERASLGLEGLGQAYLGFGG
                     TPYAEVAPKGTPFSSAPIAKAAPYCAEDAYMTLRLKEALERELADAGLSELFAGVEMP
                     VLSLLARMEREGILVDAASLEAFGKELDGELERVKAETFGLVGHEFNMNSPKQLQEIL
                     FVERKLSTGKKTKTGYSTDISVLEELAREDRVPELILRHRGLQKLRSTYVDALLALAA
                     DDPRIRTHFVQTGTATGRLSSRDPNLQNIPVREEEGRRIRKAFVAAPGKKLVSADYSQ
                     IELVVFAHLSGDPELKKAFIDGADVHRRTAALILGKAEDEVQQSERRAAKTINFGLIY
                     GMGAFRLAQELGIPRGDAQRFIDAYFERYAGVASFIRDTVETARRDGYVSTLLGRRRM
                     IRAIDSRNANERQGAERVAVNTPIQGSAADIVKLAMLRVDSTLRERFPAARILLQVHD
                     ELIIEAPEAEADAVAAAVADAMAKAYTLSVPLRVGVESAYSWGDMH"
BASE COUNT          430 a         1056 c         1041 g          512 t
ORIGIN      
        1 ggcgcgggcg agcgcggcgg ggcgacggcc gccaccgtgg cgggtgctcc tggagaggcc
       61 gcgcgcggga acgccagggc cagggccatg ccggacgcca cgacgagcgc aatcacgacg
      121 cagagcaccg tccaaagaac cttcttcgta tccatcggca tagcccctcc actacgagcg
      181 cgcctatggc gcctactacg aatatcggct cggacccgcc tccagttaag cgcccggggc
      241 cggttcccga tccacggctc cgagatcagc ggccagcgac cggaacgccc gctcggccag
      301 gccgacgacg cggcgcttga agccatcctc gcctagggcg ttccagacga cgacggtctt
      361 gcccttccag gccttcccgg cgtcgcggag cggcctctgg cttcgtatgc gccccagcat
      421 ggcgagcgcg ccgaggccgt ctctggctct gcccctgatc aggcgtaggg cgaggggcgc
      481 gcgcgcctct atgatggtat cgcaggctgc gactatgggt aagcgataga ggatcgccgc
      541 gtcgacgcag gccctggggc cggcccgact cacggcttcc tcgacgagcg cgttcatcgc
      601 cgggtggact atggcctcgt agcgggcgag caggaccgga tccgcgaaga ccatcgcgcc
      661 tatcgcccgg cgatcgggcg cgccgtcggc cttgagcgcc cctggcccga gcaggcgcgc
      721 cacctcgggc gccgatcgtt cgagcgcggc gtggccgagc gcgtcgacgt ctatgaccgt
      781 ccagccggct tcatgcaggg ctgccgccgc gctgctcttg cctgcgcagt agccgccggt
      841 cagtcccagt atcaatgcat gtccccccac gagtaggcgc tctcgactcc gacccgtagc
      901 ggcaccgata gcgtataggc cttcgccatg gcgtccgcca ccgcggcggc caccgcgtcc
      961 gcctcggcct cgggcgcttc gatgatcagc tcgtcgtgta cctggagcag tatcctggcc
     1021 gccgggaagc gctccctgag cgtcgagtcg accctgagca tcgccagctt gacgatatcc
     1081 gcggcagagc cctggatcgg cgtgttcacc gcgacgcgtt cggcgccctg gcgctcgttg
     1141 gcgttgcggc tgtcgatcgc cctgatcatg cggcgtcgcc cgagcagggt ggacacgtag
     1201 ccgtcccggc gcgccgtttc gacggtgtcc cgtatgaagg aggcgacgcc cgcgtagcgc
     1261 tcgaagtacg cgtcgatgaa gcgttgcgcg tcgcctcgcg gtatgcccag ctcctgggcc
     1321 agcctgaagg cgcccatgcc gtagatcagg ccgaagttga tggtcttggc cgctcgccgc
     1381 tccgactgct ggacctcgtc ctcggccttg cccaggatca gcgcggcggt gcgccggtgc
     1441 acgtcggcgc cgtctatgaa ggccttcttg agctcgggat cgcccgacaa gtgggcgaac
     1501 acgaccagct cgatctgcga gtaatcggcc gagacgagtt tcttgccggg cgccgccacg
     1561 aaagctttgc gtatcctgcg cccttcctct tctcggaccg gtatgttctg tagattgggg
     1621 tcgcggctgg ataggcgccc ggtcgccgta ccggtctgca cgaaatgcgt gcggatgcgc
     1681 gggtcgtcgg ccgccagcgc cagcaaggcg tcgacgtagg tactcctgag cttctggagg
     1741 cctcggtgcc ggaggatcag ctcgggcacc cggtcctcgc gcgccagctc ctcgagcacc
     1801 gatatatcgg tcgagtagcc cgtcttcgtc ttcttgccgg tggacagctt gcgctcgacg
     1861 aacagaatct cttggagctg cttcggggag ttcatgttga actcgtggcc gacgaggccg
     1921 aaggtctcgg ccttgacccg ctcgagttca ccgtccagct ccttgccgaa ggcttcgagc
     1981 gaggccgcgt cgacgagtat gccctcgcgt tccatcctgg ccaggagcga cagtaccggc
     2041 atctcgactc cggcgaacag ctccgatagg ccggcgtcag ccagctcgcg ctcgagcgct
     2101 tccttgagcc tgagcgtcat gtaggcgtcc tcggcgcagt acggggcggc tttggctatc
     2161 ggcgccgatg aaaagggggt gcccttgggc gctacctcgg cgtagggcgt gccgccgaag
     2221 cccaggtagg cctgcccgag gccctcgagg ccgagcgagg cccgttccgg gtcgacgagc
     2281 caggcggcta tcatcgtgtc ccagggggcg cagtgtatcg gggcgcccca gttttccatg
     2341 atatgcatat cgaatttaag gttctggccc acgacgacga ggtccgcgcg ggcaaggagc
     2401 ggggcgacga ggcgcctgac cgtctcggcg ctcaggcagg ccgcgtccgg ggccgcgagc
     2461 ggcacgtagc aggcggcgtc ggggctcacg gcaatcgaga agccgaccgg catgacgcgc
     2521 atctcgtcga gcgagtcggt ctcgcagtcg aacgcgaaca cgcccgcgcg ctccgcggcg
     2581 tcgacccagc gccgtagccc cgcctcgtcg aggaccagtt cgtgggttcc ggggcggccg
     2641 taggactcgg gtaggcccct cgccgccgtc ggcgatacgt cggcttcgtc tatcgtctca
     2701 gctatcgtct tgtcggcgcg cgtagccggc gcttcatgcg tcccggcctg cgtcccggcc
     2761 tgcgtcccgg cctggcttcc cgccgccgcg aacagctccc cggccgcttc gagcttgtcg
     2821 aggccgtcgg ccacgcggcg cgtcgcgagg ctacgcatac cctcgcgcat gaacacggag
     2881 ctcgccgcat ctcggtcgat agacgggacc gccagttcgt cgagcgaggc tacgggcagg
     2941 ggggcgtcgt aggctagcgt tatcagctcc ttcgagagca tggcgctgtc cctgccggcc
     3001 tcgagcttcc tgcggaggcc gtcgggcttg acctcggcg
//
LOCUS       DAOPHV010000315         3031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_45069_length_3031_cov_4.617608, whole genome shotgun sequence.
ACCESSION   DAOPHV010000315 DAOPHV010000000
VERSION     DAOPHV010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45069_length_3031_cov_4.617608"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="PLQ29_08630"
     CDS             <1..966
                     /locus_tag="PLQ29_08630"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HPG86751.1"
                     /translation="VADDAARDRLEIMPAYWWMYNMYALLRNESKFVARDKRQSRGQN
                     IEFSPFAPDIAEEAFGALELLERFVGVAWYRASGGAEAVVEGSDDEATRAKGRELLLG
                     PKEDVDALEVLGYGIENSRRRVLIQKPRRAYKAYRHLLRWYAMRVLIEYFAERPDATF
                     QDAGDVLEGERVREWENLGGQLVPRSKVEALVADVKDGKVGGWGEMHAEYARLWSEYP
                     LDKAQHAWATMCDLLGVERLEEAAFAKEVVRFMDTTRFIEEQVYLTRRKDYVNGFRLA
                     TFRGEAEMRAVLGSPESAPFVRQSRADMERWRARADALLGRLSAE"
     gene            complement(1001..1468)
                     /locus_tag="PLQ29_08635"
     CDS             complement(1001..1468)
                     /locus_tag="PLQ29_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86752.1"
                     /translation="MKGKRDVPEMSNADLKRMLDQTMIRFENWRVEVERKPNPMMIHS
                     LKNIMSIIETVTSMADRRIEYSYKALVTRMAELMHETAELNMTIVNSLQGDDSIDEDE
                     LKLVSSRLFTLINAAAELARLVQEHFGSPELTAATGRNLLPEPGDDTAQTKRR"
     gene            complement(1648..2742)
                     /gene="serC"
                     /locus_tag="PLQ29_08640"
     CDS             complement(1648..2742)
                     /gene="serC"
                     /locus_tag="PLQ29_08640"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HPG86753.1"
                     /translation="MKRVINYSAGPAGIPVEVLEQARDEMLDWNGTGCSVMEVSHRGK
                     DYDALHMGTMDLFRELAGLGPDYNVLFTTGGASTQFLMLPMNYLGAGKEADYIVTGNW
                     SQAAVKEAKRYGKITHPADMHLGDGMFVAIPKQGELKLNPAAEYVHYTSNNTVEGTEW
                     HYVPDVKGKPLVCDMSSDIFSRPVDFKKFSMIYAGAQKNLGPSGVTVVAVKDDFLQKA
                     HDDLPTMLSYKVFKEKFSMHNTPPCFSIYILNLVLRWLKKNGGLEAMGKINDQKQRLL
                     YSAIDGSAGYFKGTANADSRSWMNVDFKLPTPELDAKFVSDAKAAGIVQTKGYRSMGG
                     IRLSCYNAVSLKDVETTVQFMQDFKAKNPA"
     gene            complement(2755..>3031)
                     /locus_tag="PLQ29_08645"
     CDS             complement(2755..>3031)
                     /locus_tag="PLQ29_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPG86754.1"
                     /translation="AKALASMKKGAYLVNTCRGKVVFEEDIVAALESGQLAGYGTDVY
                     YSDPPENTPLAKAPHVVMTPHLGASTAENLDRLGDCIVERVRKYCKK"
BASE COUNT          489 a          901 c         1054 g          587 t
ORIGIN      
        1 gtggccgacg acgcggcccg cgacaggctc gagatcatgc ccgcctactg gtggatgtac
       61 aatatgtacg cgctcctgcg caacgagagc aagttcgtcg cgcgcgacaa gcggcagagc
      121 cgcggccaga acatcgagtt ctcgcccttc gcgcccgaca tcgccgagga ggccttcggc
      181 gccctcgagc tgctcgagcg tttcgtcggg gtggcgtggt accgcgcgtc cggcggcgcc
      241 gaggccgtcg tcgagggatc cgacgacgag gcgacccggg ccaagggccg agagctgctg
      301 ctcggcccca aggaggacgt cgacgcgctc gaggtcctcg ggtacggtat cgagaattcc
      361 aggcgacgcg tgctgatcca gaagcccagg cgcgcctaca aggcgtatcg ccatctgctc
      421 cgctggtacg cgatgcgcgt gctcatcgag tatttcgccg aacggccgga cgccaccttc
      481 caggacgccg gcgacgtgct cgagggcgaa cgggtgcgcg agtgggagaa cctcggcggg
      541 cagctcgtgc cgcgctcgaa ggtcgaggcc ctggtcgccg acgtcaagga cggcaaggtg
      601 gggggctggg gcgagatgca cgccgagtac gccaggctat ggtcggagta cccgctggac
      661 aaggcccagc acgcctgggc gacgatgtgc gacctgctcg gcgtcgagcg cctcgaggag
      721 gccgcgttcg ccaaggaggt ggtcaggttc atggacacca cccgcttcat cgaggagcag
      781 gtttacctga ccaggcgcaa ggactacgtc aacgggttca ggctggccac cttccgcggc
      841 gaggcggaga tgcgggccgt gctcggctcg ccggagtcgg ccccgttcgt caggcagtcg
      901 cgggccgaca tggagcgatg gcgcgcgagg gcggacgcct tgctcggtcg cctatcggcc
      961 gagtaggcgg ggataggagc cgtcggctcg cggaggcgcg ctagcgcctc ttcgtctgcg
     1021 ccgtatcgtc gccgggctcc gggagcaggt tccgccccgt cgccgcggtc aactccggcg
     1081 agccgaagtg ctcctgcacg agccgggcca gctcggccgc cgcgttgatc aatgtgaaca
     1141 gtcggctcga tacgagcttc agctcatcct cgtcgatcga gtcgtcgccc tggagggagt
     1201 tgacgatggt catgttgagc tcggccgtct cgtgcatcag ctcggccatg cgcgtcacca
     1261 gggccttgta ggagtactcg atccgccggt cggccatcga ggtgaccgtc tcgatgatcg
     1321 acatgatgtt cttgagcgaa tggatcatca tcgggttggg cttgcgttcc acctcgacgc
     1381 gccagttctc gaaccgtatc atcgtctggt cgagcatgcg cttgaggtcg gcgttgctca
     1441 tctccggtac gtcgcgcttg cccttcatgg attaacctgc ctgaacggag agaagaagaa
     1501 tcagaccacg gaggcacgga ggcacgaagg gaaatgaggt caagatagag cgcaaaggaa
     1561 tattttcctt tctcatctct catcctccat tttcttcagc tccgtgcctt cgtgtctccg
     1621 tggtaacgtt tttaagcttg cattatatca ggccgggttc ttggccttga agtcctgcat
     1681 gaactgcacg gtcgtctcga cgtccttgag gctgaccgcg ttatagcagg acaggcggat
     1741 gccgcccatc gagcgatagc ccttggtctg gactatcccg gcggccttgg cgtcggagac
     1801 gaacttggcg tcgagctcgg gggtcgggag cttgaagtcc acgttcatcc aggagcgaga
     1861 gtcggcgttg gcggtgccct tgaagtagcc cgcggagccg tcgatcgcgg agtacaggag
     1921 ccgctgcttc tgatcgttga tcttccccat agcctcgagg ccgccgttct tcttgagcca
     1981 gcgcagcacg aggttcagga tgtagatcga gaagcagggc ggggtgttgt gcatggagaa
     2041 cttctccttg aacaccttgt aggacagcat ggtcggcagg tcgtcgtggg ccttctggag
     2101 gaagtcgtcc ttgaccgcga ccacggtgac tccggacggg ccgaggttct tctgggcgcc
     2161 ggcgtagatc atgctgaatt tcttgaagtc gaccgggcgg ctgaagatgt cgctcgacat
     2221 gtcgcagacg agaggcttgc ccttgacgtc ggggacgtag tgccactcgg taccctcgac
     2281 ggtgttgttc gaggtgtagt ggacgtactc ggcggccggg ttgagcttga gctcgccctg
     2341 cttggggatg gcgacgaaca tgccgtcgcc gaggtgcatg tcggcggggt gggtgatctt
     2401 gccgtagcgt ttggcttcct tgacggcggc ctgggaccag ttgccggtga cgatatagtc
     2461 ggcttccttg cctgcgccga ggtagttcat cggcagcatc aggaactggg tggacgcgcc
     2521 gccggtcgtg aacagcacgt tatagtcggg gccgaggccg gccagctcgc ggaacaggtc
     2581 catcgtgccc atgtgcaggg cgtcgtagtc cttgccgcgg tgcgagacct ccatgacgga
     2641 acagccggtg ccgttccagt cgagcatctc gtcgcgggcc tgctcgagaa cctctacggg
     2701 gatgccggcg gggccggccg aatagttgat tacgcgtttc atggtatagc ctccttattt
     2761 cttgcagtac ttccgtacgc gctcgacgat gcagtcgccg agccggtcca ggttctcggc
     2821 cgtcgaggcg ccgaggtgcg gggtcatgac gacgtggggc gccttggcga gcggcgtgtt
     2881 ctccggcggg tcggaatagt acacgtcggt gccgtagccg gccagctggc cgctctcgag
     2941 cgcggcgacg atgtcctcct cgaagacgac cttgccgcgg caggtgttga cgaggtaggc
     3001 gcccttcttc atcgaggcga gggccttggc g
//
LOCUS       DAOPHV010000316         3028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_45147_length_3028_cov_2.967709, whole genome shotgun sequence.
ACCESSION   DAOPHV010000316 DAOPHV010000000
VERSION     DAOPHV010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45147_length_3028_cov_2.967709"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PLQ29_08650"
     CDS             complement(<1..425)
                     /locus_tag="PLQ29_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86755.1"
                     /translation="MDTYRPTEAWAFAQLDDFWERYGPLTPWGKDAAEARPFIGDRDE
                     LERRYDDVEAALGFLADREGDPSSLDRASYHLRRMPRLALEAKSSYELIELFQIKKFI
                     ANYRGARSVLGEPLALRFGMEGLADGSAAKALAVELDRGG"
     gene            complement(425..1324)
                     /locus_tag="PLQ29_08655"
     CDS             complement(425..1324)
                     /locus_tag="PLQ29_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86756.1"
                     /translation="MLDRPEDFLGRVTFVTGPEKHCGKTTFMNRAALLARRGAAASGR
                     PGPALLTVGYDGEARDLLSGARKPAVPVERGDLIVTTERFARPCSPEILEALPGSTAL
                     GRLCVARAGRPATVALVGPEGNSLVAWAVRRILDEGLGETVLVDGAINRVTQAASIEG
                     ARFVYALRVDAAGLERSVSRVRRMAALVDLPLAGATADESAPGHVVMAGALTAETAAR
                     LPAAAESVVVDDFTKIFLSDAELAAFTRGRRLLVARRVEFGGFVVACRGLTDDAFLER
                     LADDSIASMVAFNPYEERPGRAA"
     gene            complement(1430..1807)
                     /locus_tag="PLQ29_08660"
     CDS             complement(1430..1807)
                     /locus_tag="PLQ29_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HPG86757.1"
                     /translation="MIIGDDSIGAKVIGCSIAVHRELGPGLLESIYEEALSIELGRSG
                     IPFVRQMELPVIYKDVQLSGAFRVDLLVDNTVVVELKCVDRILPVHEAQVLSYLRLGG
                     WRIGLLINFKSAVLKNGIRRVLL"
     gene            complement(1861..>3028)
                     /gene="ablA"
                     /locus_tag="PLQ29_08665"
     CDS             complement(1861..>3028)
                     /gene="ablA"
                     /locus_tag="PLQ29_08665"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HPG86758.1"
                     /translation="ENDTVKEKADISEVLKVFRMAITPYYASLIDPANPGCPVRLQAI
                     PRLPETHFDESDQDDPLHEDSDSPVPGLTHRYPDRVLLLVTHICSMNCRHCTRRRIVG
                     EEDANMLDHQVDAAIEYIRKTPIVRDVLISGGDPLVLSDDKLERIIKKIREIPHVEMI
                     RIGTRTPVVMPQRITDDLVNMLKKYHPLYINTHFNHPKEITAEAREACRKLADAGIPL
                     GNQSVLLRDVNDCPVLMKQLVQKLLTIRVRPYYIYQCDLSRGISHFRTTVSKGIEIIE
                     NLRGHTTGLAVPTFVVDAPGGGGKTPVMPDYLISQNDKRVILRNYEGVITTYDEPDGY
                     HEDCGKCSICSDEPQLNATCGVARLLSGDDKTLEPKNLARAARGGTKFIPRKRK"
BASE COUNT          508 a          952 c         1027 g          541 t
ORIGIN      
        1 ccgccgcggt ccagctccac agcgagagcc ttggcggccg aaccatcggc caggccctcc
       61 atgccgaagc gcagggccag cggctcgccg agcaccgaac gggcgcccct gtaattggct
      121 atgaatttct ttatctggaa cagctcgatc agctcgtacg agctcttggc ctcgagggct
      181 agcctgggca tacgcctgag gtggtacgaa gcgcggtcga gcgacgacgg gtcgccctcc
      241 cggtcggcca ggaagccgag ggccgcctcg acgtcgtcgt agcggcgctc cagctcgtcg
      301 cggtcgccga tgaacgggcg ggcctcggcg gcgtccttgc cccagggcgt caagggtcca
      361 tagcgctccc agaaatcgtc gagctgggcg aaggcccagg cctcggtggg cctgtacgta
      421 tccatcacgc ggccctcccc ggccgttcct cgtaggggtt gaacgccacc atcgaggcta
      481 tcgagtcatc ggccagccgc tccaggaagg cgtcgtcggt caggccgcgg cacgcgacga
      541 cgaagccgcc gaactcgacc cgcctggcga ccaggagtcg ccgcccccgc gtgaaggccg
      601 ccagctccgc gtcggacaga aagatcttgg tgaagtcgtc gacgacgacc gattcggcgg
      661 cggcgggcag cctggcggcg gtctcggcgg tcagcgcgcc ggccatgacg acatggccgg
      721 gcgcgctctc gtcggccgtc gcgccggcca aagggaggtc gacgagcgcg gccatgcgcc
      781 tcacccgcga taccgagcgc tcgaggccgg ccgcatcgac gcggagcgcg tagacgaagc
      841 gcgccccctc tatggaggcg gcctgagtca cgcggttgat agcgccgtcg accagtacgg
      901 tctcgcccag cccctcgtcg aggatgcgcc tgacggccca ggcaaccaga gaattgccct
      961 ccgggccgac cagggcgacg gtcgcgggcc ggccggcccg ggcgacgcag aggcggccga
     1021 gcgctgtaga cccaggcagc gcctcgagga tctccggcga gcacggcctg gcgaagcgct
     1081 ccgtggtgac gatgaggtcc ccccgctcga ccggcaccgc cggcttgcgg gcgccggaca
     1141 gcaggtcgcg ggcctcgccg tcgtagccga cggtcagcag ggccggcccc ggtcgcccgg
     1201 acgcggccgc gcctcgtcgt gccaggagcg cggcccggtt catgaacgtc gtcttgccgc
     1261 agtgtttctc gggtcccgtc acgaacgtaa ccctgccaag gaaatcctcg ggcctatcga
     1321 gcatggcgat gcccttctga cgaattgaac cacggagaca cggaggcacg gaggatgcga
     1381 aacgctttaa ggccaaagca caccatgatt gtagagcttg gagtacggct cataatagga
     1441 ccctacggat cccgttctta agtacagcgg atttgaaatt gataagcaag ccgattctcc
     1501 atccacccaa gcgtaagtac gataaaactt gagcctcatg aaccgggagg atcctatcaa
     1561 cgcacttcaa ctccaccacc acggtgttgt cgacaagcaa atccaccctg aatgcccccg
     1621 agagctggac gtccttatag ataacgggta gctccatttg ccgaacgaag ggaatcccgg
     1681 aacggccgag ttcgatactg agggcttcct cgtagataga ctcaagcaag cccggtccga
     1741 gctcacggtg tacggcgatg gaacaaccga tcaccttagc gcctatcgag tcatcaccta
     1801 tgatcattgc gccatctccg tgactccgtg tctccgtggt tcgggtttct cgtatcccgg
     1861 ttacttccgc ttgcgcggga tgaacttggt accgcccctg gcggccctgg cgaggttctt
     1921 gggctcgagc gtcttgtcat ccccggacag gagcctggcg acgccgcagg tggcgttgag
     1981 ctggggctcg tcggagcaga tcgagcactt gccgcagtcc tcgtggtagc cgtccggttc
     2041 gtcgtaggtc gtgatgacgc cctcgtagtt acgcaggatc acgcgcttgt cgttctgtga
     2101 aatcaggtag tccggcatta ccggagtctt gccgccgccg ccgggtgcgt cgaccacgaa
     2161 ggtcggcacc gcgaggccgg tcgtatggcc gcgcaggttc tcgatgatct cgatgccctt
     2221 ggacaccgtg gtgcggaaat ggctgatgcc gcgcgacagg tcgcactggt agatgtagta
     2281 cgggcgcacg cggatggtca ggagtttctg cacgagctgt ttcatgagaa cggggcagtc
     2341 gttgacgtct cgtagcagca cgctctggtt cccgaggggg atgccggcgt cggccagctt
     2401 gcggcaggcc tcgcgggcct cggcggtgat ctccttggga tggttgaaat gcgtgttgat
     2461 gtacagcggg tggtacttct tgagcatgtt cacgaggtcg tcggtgatgc gctggggcat
     2521 gaccaccggc gtgcgggtac cgatgcgtat catctccacg tgcgggatct cgcgtatctt
     2581 cttgatgatg cgctcgagct tgtcgtcgga cagcacgagg gggtcgccgc cggagatcag
     2641 cacgtcgcgc acgatcggcg tcttccggat gtactcgatg gccgcgtcga cctggtggtc
     2701 gagcatgttc gcgtcctctt cgccgacgat gcggcggcgg gtgcagtggc ggcagttcat
     2761 cgagcagata tgcgtgacga ggagcagtac gcggtcgggg tatcggtgcg tgaggccggg
     2821 cacggggctg tcagagtcct cgtgcagggg gtcgtcctgg tcgctctcgt cgaagtgcgt
     2881 ctccggcagg cgcgggatgg cctggagcct gaccgggcag ccgggattgg ccgggtcgat
     2941 gagcgacgcg tagtatggcg tgatcgccat gcggaagacc ttgagcacct cggagatgtc
     3001 ggccttctcc ttgaccgtat cgttttct
//
LOCUS       DAOPHV010000317         3011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_45500_length_3011_cov_3.732070, whole genome shotgun sequence.
ACCESSION   DAOPHV010000317 DAOPHV010000000
VERSION     DAOPHV010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45500_length_3011_cov_3.732070"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PLQ29_08670"
     CDS             <1..438
                     /locus_tag="PLQ29_08670"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPG86759.1"
                     /translation="PKHPDELLDKMSFGSYNKAFKPNRTVFDWLSRDEAEVDAYIADP
                     FCGFLCTYGFFRDLLGGLAWIHAPATMAGVPTSLPIYMFAGAKDPVGAANGSFDWLYN
                     RYKELGVVDLSRKLYPDGRHEVFNDTCRDEATADVIAWLDARA"
     gene            565..1704
                     /gene="pcnB"
                     /locus_tag="PLQ29_08675"
     CDS             565..1704
                     /gene="pcnB"
                     /locus_tag="PLQ29_08675"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HPG86760.1"
                     /translation="MLVRYQNGPDGKPVKKAIVYTEREHGIDPRSVDQDAVRIVERLR
                     RSGHAAYIVGGAVRDLLLGREPKDYDIVTDAEPPRIKKLFYRSRIIGKRFRLVHVYVG
                     ERIFEVSTFRSIANGTVGNTYGTIDEDAKRRDFSMNALYFDPLEQKLVDYVGGLKDMR
                     ARKIVPVIPLKTIFAEDPVRMIRAVKYAEATGFAIPFKTRLAIRRHAPALATASISRL
                     GEEANKILYSGKARGIVESLRRFGLLSAIMPGVDKALASEGAAAASMKAALAELDAYV
                     AATGDKSLGALLAYVLRDAVAAAQAIPAPDAQAAFHNALEAARDFLAPLTMPRIEVER
                     AVKSVFKAPEPKQKPKRPRRRRRRKPPTGAAEPSPRGGDEPRDGE"
     gene            complement(1843..2313)
                     /locus_tag="PLQ29_08680"
     CDS             complement(1843..2313)
                     /locus_tag="PLQ29_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86761.1"
                     /translation="MGQQSRAIPETGVQIDVVRATLVGQMQIPAVVVFTHDHLERASG
                     LALGDRFLDDAEEQARERRIGAQLVERVVLHVPEHEGPLGIPSYHVDDSLVVAALDAR
                     GGVEGCVDLVEFGAGILLFLDMDRCVAGSLPLLAVFLECLEQGFDDEDAFQLDR"
     gene            complement(2664..>3011)
                     /locus_tag="PLQ29_08685"
     CDS             complement(2664..>3011)
                     /locus_tag="PLQ29_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86762.1"
                     /translation="PGGGGPTGALFDNRATETVQALLGKADRVVAPPGWPDTLVALYG
                     KGRLFLDRASLDEAFPPGQEFQLGFPDKTASRMGEWVAAWITGLSDGSALASAQVIRR
                     PELQRWIGSWEKR"
BASE COUNT          550 a          996 c          958 g          507 t
ORIGIN      
        1 cccaagcatc ccgatgagct gctcgacaag atgagcttcg gttcgtataa caaggccttc
       61 aagccgaacc gcacggtatt cgattggctg tcgcgagacg aagccgaggt cgatgcatac
      121 attgccgacc ccttctgcgg tttcctatgc acctacggtt ttttcaggga cctgctcgga
      181 ggcctggctt ggattcatgc tccggccacg atggcgggcg tacccacgtc gttgcccatc
      241 tatatgttcg ccggggccaa agaccctgtc ggggcagcca acggctcttt cgactggctg
      301 tacaatcgct acaaggagct gggcgtcgtc gacctatcca ggaagctata ccccgacggc
      361 cgtcacgagg tgttcaacga cacctgccgc gacgaggcga ccgccgatgt catagcctgg
      421 ctcgacgcca gagcctgacc gaggcaacgc cgtagccccg taccggcttg acctgtaccg
      481 ccggtgcggg ggtatagtac agtacccggg ccagcggccc agtcgctctc gtagcgatcc
      541 gcacatctat ctctagagga aaccttgctc gtacgatatc agaacgggcc ggatggaaag
      601 ccggtcaaga aagccatagt ctatacggaa cgcgaacacg gcatcgatcc gcgttccgtg
      661 gatcaggacg cggtgcgcat cgtcgagcgc ctgaggcgct ccggccacgc ggcttacatc
      721 gtcggcggcg ccgtccggga tctgctcctc ggccgcgaac ccaaggacta cgacatcgtc
      781 accgacgccg agccccctcg gatcaagaag ctgttctatc gctcgaggat catcggtaaa
      841 cgcttccggc tcgtccacgt ctacgtcggc gagcgcatct tcgaggtctc gaccttccgt
      901 tcgatagcca atggcaccgt cggcaacacc tatggcacca tcgacgagga cgcgaaacgc
      961 cgcgatttca gcatgaacgc gctgtacttc gacccgctcg agcagaagct cgtcgactac
     1021 gtcggcggcc tcaaggacat gcgcgccagg aagatagtgc cggtcatccc gctcaagacc
     1081 atcttcgcgg aggaccccgt gcgcatgatc cgggcggtca agtacgccga ggccaccggt
     1141 ttcgccatcc cgttcaagac gcggctggcc atacgccggc acgcccccgc gctggctacc
     1201 gcctcgatca gccggctcgg cgaggaagcc aataagatac tctactccgg gaaggcgcga
     1261 ggcatcgtcg agtccttgcg ccgcttcggc ctcttatcgg cgatcatgcc gggcgtcgac
     1321 aaggcgctcg cctcggaggg agccgcggcc gcgtcgatga aggcggccct cgccgaactc
     1381 gacgcctatg tcgcggccac cggggataag tcgctcgggg cgctcctggc ctatgtgctc
     1441 agggacgccg tagcggctgc ccaggccatt ccggctcccg acgcccaggc cgccttccat
     1501 aacgcgctcg aagccgcccg ggatttcctg gctccgctga ccatgccgcg gatcgaggtg
     1561 gagcgggccg tcaagtcggt cttcaaggcc ccggaaccga agcagaagcc taagcgcccg
     1621 agacgccgcc gccggaggaa gccgccgacc ggcgccgcag agccgtcccc taggggcggc
     1681 gacgagccgc gcgacggtga ataggccccc tgaggatgcg tttcgcccga tggcttgaca
     1741 cggcgaggcc atttctcgga tgatgcgacg gtgaacgtcg aacggctgaa cggattatcg
     1801 attgacgccc tctacgccct cgcggacaag atggggctcg atttaccgat cgagttggaa
     1861 cgcgtcttcg tcgtcgaagc cctgctcgag gcattcgagg aagacagcga ggagcggaag
     1921 gcttccggcg acgcagcggt ccatatcgag gaaaagaagt attccggctc cgaactcgac
     1981 gagatcgacg catccctcga cgccgcccct tgcatcgaga gccgctacaa cgagactgtc
     2041 gtccacgtga tagctcggga tcccgagtgg gccttcgtgt tctgggacgt gaaggacgac
     2101 gcgttcgacg agttgcgcgc caatcctgcg ttctcgtgcc tgttcctccg cgtcgtcaag
     2161 gaatcggtcc ccgagggcaa gaccgctggc tcgttcgagg tggtcgtggg tgaagacgac
     2221 gaccgctggt atctgcatct gcccgacgag ggttgctcgt accacgtcga tctgtacgcc
     2281 cgtctcggga atcgctcgcg attgctggcc cactcggccg tcgtcaggac gccgagggcg
     2341 ttgatggccg gctcggaagc accgccgaga agctggtacg cgaaaaagcg caccggcagg
     2401 cgcgactcga gccagcggtc gaccggctgc tgcttgggcg cgccaaggtt gtcgaaagtg
     2461 aagtcgagga cgcggacgca ggcggggggc gccagccgcc gcgccagatc cattccggca
     2521 ggcttctgcc cggcagcggc gctgccgagc aggagtcccg cccccatgag ccagccgaac
     2581 ccgctctgga tcagtgcatt acgcatagcg gtctccctgt caggcaaggc cgaggttgat
     2641 ttcacgagtg aaagcgcgcg ggatcatcgc ttttcccacg agccgatcca gcgctgcagt
     2701 tcgggccttc tgatgacctg tgcggatgcg agcgccgacc catccgacag gcccgtgatc
     2761 catgccgcaa cccattcgcc catgcgcgag gccgtcttgt cggggaaacc gagctggaat
     2821 tcctggccgg gcgggaaggc ttcgtcaagc gatgcacgat cgaggaaaag ccggcctttc
     2881 ccgtagaggg caaccagcgt gtccggccag ccgggcggcg cgacgacgcg atctgccttg
     2941 cccaggagag cctgcaccgt ctccgtcgcc cggttgtcga agagcgcgcc ggtcggtccg
     3001 ccgccgccgg g
//
LOCUS       DAOPHV010000318         3004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_45647_length_3004_cov_3.566633, whole genome shotgun sequence.
ACCESSION   DAOPHV010000318 DAOPHV010000000
VERSION     DAOPHV010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45647_length_3004_cov_3.566633"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(349..552)
                     /locus_tag="PLQ29_08690"
     CDS             complement(349..552)
                     /locus_tag="PLQ29_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86763.1"
                     /translation="MVGTELVTIRDFERYLVQQGASKHTAMVAASVAWRQLEPEPAET
                     RALCAYVLGETLAACLAERRPSN"
     gene            complement(558..968)
                     /locus_tag="PLQ29_08695"
     CDS             complement(558..968)
                     /locus_tag="PLQ29_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86764.1"
                     /translation="MDFLTRAKIVRLSGAVSAGATNIDCTPEVDCTGYESAVIIATFG
                     TVVDTAVCGLKARLGDASGALTVAATPAASFIASTSSQKQLAIEVKRPNGKLISARIE
                     RTTANAALESCVLLLFDHRQGPSTMGDLLARAIG"
     gene            complement(985..2130)
                     /locus_tag="PLQ29_08700"
     CDS             complement(985..2130)
                     /locus_tag="PLQ29_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HPG86765.1"
                     /translation="MNEELQEAITDLGSSMKDFRARMETRQAELEARVNRMNLGTAGA
                     GKLGSSPEPQDRGFFLQAERIASAAPAYRLGELVRAAVTGKATTEVRNVLLEGGSVGG
                     GYLVPESTVSVVIDAMRDALVCQKAGCKFVTMTEPGLAYPRVDTPPTATWTGENSAAT
                     EASMAFGRVDLRARKLVALIRASRELIDDAPAFPTLIDQLFSQALASGFDSAALIGSG
                     KGETPLGIANTPGIGVKDLSAALTLDDILEAYYSLLGENVPEDSVSCVMNSLVAKKMG
                     LLKSDATTDKYLLSEAPPKEFERIKFFVTNAIPTASNKSTIIMGDFSRLVLGVRLDGI
                     LEISPAAVIAGESTFERSQIAIRGTLRGDVAVEDPHAFGIITSVPTN"
     gene            complement(2127..2597)
                     /locus_tag="PLQ29_08705"
     CDS             complement(2127..2597)
                     /locus_tag="PLQ29_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86766.1"
                     /translation="MDFQRALESEILSAANKGRDTKFTPDDMLGAMLDIMTTIGADER
                     VALLEKGRRFNASFTYPAYVSALGLIRRSKEILAIAIENGVLDADYAIGDDDELDAVH
                     TAEIREKGWSPYYSVRKEAGKHSGGWVDEYSPSLDKIEATLAAIAALDGKEQSK"
     gene            complement(2631..>3004)
                     /locus_tag="PLQ29_08710"
     CDS             complement(2631..>3004)
                     /locus_tag="PLQ29_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86767.1"
                     /translation="TQAEIAGALGVSQPTVSRLVKLARGAGLIPARLARREKTLDRHM
                     PDEAFLEVISNPYFLAGVSKLARRYCGVFRRQYALDFDDLRQMAFESLLGKEPGQAVG
                     FYLQAVEWSFQKILRDDRTPS"
BASE COUNT          534 a          943 c          918 g          609 t
ORIGIN      
        1 cctggtccag cctatccaac accattgcgg atactcacaa ggttgcggcc gctaacctgc
       61 ctgataatga cgttaccatt atcaatcagg cgattgaccg gagcttacgg aacgacctgt
      121 gcgacgttgc ataggtacat cgggcaacga ttgccacggg gtagcgtttc gcgaccccat
      181 cgacgaccac tgtcagtgag ggggtgaacg tttcgttcac ccccttcaga taccggattg
      241 cttctaggaa tagagctccg tccgcccaag atgaggccta caaccgaagt tgcaggtgcc
      301 gctaccaaaa ggggtatgaa aaggggcggc aagctgccgc cccgtcatct agtttgaagg
      361 tctccgttcg gccaggcagg cggctagcgt ctcgcccagg acgtaagcgc atagcgcccg
      421 tgtctcggcc ggctccggct ctagctgccg ccaagcaacc gacgccgcga ccatggccgt
      481 atgctttgaa gcgccctgct ggactaggta gcgctcgaag tcgcggatgg ttaccagttc
      541 cgtgccgacc atctatctta ccctatcgcc ctcgccagca ggtcgcccat ggttgagggg
      601 ccttgcctgt ggtcgaatag gagcaggacg cagctctcga gcgctgcgtt cgctgtcgtg
      661 cgttctatcc gggcggaaat cagctttccg ttcggcctct ttacctcgat agcgagctgc
      721 ttctgcgagc tcgtcgaggc gatgaaggac gccgcgggag tagcggcaac ggtcagcgcg
      781 ccgctggcgt cgcccagccg cgccttcagg ccgcacacgg ccgtgtccac gacggtcccg
      841 aaggtggcga tgatgaccgc cgactcgtag ccggtacaat cgacttccgg cgtgcagtcg
      901 atgttcgtcg cgcctgcgga aaccgctccc gatagacgta ctatcttcgc cctggtcaag
      961 aaatccatgc ttcaccgtcc tttcttagtt cgtcggtacg ctcgtgataa tcccgaaggc
     1021 atgggggtcc tcgaccgcga catcgccgcg gagcgtgccg cgaatggcta tctgcgagcg
     1081 ctcgaacgtg ctttcgcctg cgatgaccgc tgcgggggaa atctcgagga taccgtccag
     1141 gcgtaccccg aggacgagcc tggagaaatc gcccatgatg atggtgctct tgttcgaggc
     1201 cgtgggaatg gcgttggtga cgaagaactt gatgcgctcg aactctttcg gcggcgcttc
     1261 ggacaggagg tacttatccg tcgtggcgtc ggacttcaag agtcccatct ttttggcgac
     1321 gagcgagttc atgacgcagg agaccgaatc ctccgggacg ttctcgccga gaagggagta
     1381 gtaggcctcc aggatgtcgt ccagggtaag cgcggcagat aggtctttca cgccgatgcc
     1441 tggagtgttg gcgataccca gcggcgtctc gcccttgccg gagccgatca gggcagccga
     1501 atcgaagccc gaggctagcg cctggctgaa cagctgatcg ataagggtgg ggaacgccgg
     1561 cgcgtcatcg ataagctccc gcgacgcgcg gatcagggcg accagcttgc gggcgcggag
     1621 gtcgacgcga ccgaacgcca tgctggcctc ggtcgccgcg ctattctctc cggtccaggt
     1681 cgccgtcggc ggcgtgtcga cgcggggata cgcgagcccc ggctcggtca tggttacgaa
     1741 tttgcagccc gccttctggc atacgagcgc gtcgcgcatc gcgtcgatga cgacgctgac
     1801 cgtgctttcc gggacgaggt acccaccgcc gacggaaccg ccctcgagga gcacgttgcg
     1861 aacttccgtc gtcgccttgc cagtcacggc ggcgcggacg agttcgccca gcctataggc
     1921 gggtgccgcg gaggcgatcc gctctgcctg gaggaagaac ccgcgatcct ggggttccgg
     1981 cgagctgccg agcttaccgg ccccggccgt gcctaggttc atgcgattga cccgggcctc
     2041 gagctccgcc tgtcgggttt ccatcctggc ccggaaatcc ttcatcgagc tacccagatc
     2101 ggtgatagcc tcctgcagtt cctcgttcac ttcgattgct cctttccgtc gagagccgct
     2161 atcgcggcca atgtcgcctc gatcttgtct agcgaaggtg aatactcgtc gacccacccg
     2221 cccgagtgct tgccggcttc cttgcggacc gagtagtacg gcgaccagcc cttttcccgg
     2281 atttccgcgg tgtgcacagc gtccagttcg tcgtcgtcgc cgatcgcgta gtccgcgtcc
     2341 aggacgccgt tttcgatcgc gatggcaagg atctccttgc ttcgcctgat cagccctaga
     2401 gccgacacgt acgccggata ggtgaacgaa gcattgaacc ttcggccttt ttccagcagt
     2461 gcgacccgtt cgtcagcgcc tatggtcgtc atgatgtcca gcatcgcgcc gagcatgtcg
     2521 tccggcgtga atttggtatc acgacccttg tttgcggcgg acaggatctc gctttccagg
     2581 gcacgttgaa aatccaccgt ttgcctcctg ccttatctaa tccgttttat tcacgatggc
     2641 gttcgatcat cccggagaat tttctggaag ctccattcga ccgcctgaag gtagaagcca
     2701 acggcctggc cgggttcttt accgaggagg ctctcaaagg ccatctggcg aagatcgtca
     2761 aagtcaagcg catactgacg ccgaaagacg ccgcagtatc ttcgcgccaa cttggacacc
     2821 cccgcgagga aatacgggtt gctaatgacc tcgaggaatg cctcgtccgg catatggcga
     2881 tcgagggtct tctctcgtcg ggccagcctg gccgggatga ggccggcccc gcgcgcaagc
     2941 ttcaccaggc gtgaaacagt tggctgagac acgccgagcg ccccggcgat ttcagcctgc
     3001 gtcg
//
LOCUS       DAOPHV010000319         2999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_45753_length_2999_cov_5.019701, whole genome shotgun sequence.
ACCESSION   DAOPHV010000319 DAOPHV010000000
VERSION     DAOPHV010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45753_length_2999_cov_5.019701"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1444
                     /locus_tag="PLQ29_08715"
     CDS             23..1444
                     /locus_tag="PLQ29_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015485 - cholesterol binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-activated cytolysin family protein"
                     /protein_id="HPG86768.1"
                     /translation="MVDTPVPESAVAEPDEQGVDETGAPVTYKITTTKYKASATYDTN
                     VLLNPSTDVIYPGSVLLGESIDDGSYIEITGGEKRPVTISFDLSGVDGDVTGKIVPTL
                     SNYRELKNKILGQSMPRQTSTYSFEKIEINSESELGVKVSAGASFDGGVYAASVKNSF
                     SFDNSKKTNKVMVKFMQTFYTVDVDQGTFLYKSYDLAEFGGYRPVYVSSIAYGRLAYI
                     TIESTESIEAINNNLEAAFKYGNAEAEASAEVAKSWLSSKAQTNITVIGGSTVAVDLE
                     SFMAMLQEDVFSSDNPGTIIAYKLRFVDDNTVANTIFNGDYTIRKAEAVKGKGINLTL
                     RITGLNAQADDTNGNLELYGRIHFKSGSQSEDLLNVANTDYMQLPKIYTNYPDLSTCD
                     WWKGSAKTLNLASTTTDFTIETSAFGEVDDGANEKFIDTVNPLTVSALTNGKAFKIKC
                     DMVGYVGKQYLEFTIVPTIEYLY"
     gene            1521..2213
                     /locus_tag="PLQ29_08720"
     CDS             1521..2213
                     /locus_tag="PLQ29_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86769.1"
                     /translation="MMYTTEVEHMCPLAKGSYHGPAPIPEEGKWVQAKETKDISGFTH
                     GVGWCAPQQGACKLSLNIKEGVIQEALVETIGCSGMTHSAAMAAEILIGKTIIEAMNT
                     DLVCDAINTAMRELFLQIAYGRTQTAFSEGGLPIGAGLEDLGKGLRSQIGTIFSTKAK
                     GVRYLELAEGYVNQLALDTDDQIIGYEIVHLGKMMDMIKKGTDANDALKKATSTYGRF
                     DDAARKIDPRHA"
     gene            2230..>2999
                     /locus_tag="PLQ29_08725"
     CDS             2230..>2999
                     /locus_tag="PLQ29_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HPG86770.1"
                     /translation="MALFESYERRIKQINAVLGAHGITSIEETKSICDAKGIDVAAIV
                     RGIQPICFENACWAYTVGAAIAIKKGVTAAPEAAEILGQGLQSFCIPGSVADDRKVGI
                     GHGNLAAMLLREETKCFAFLAGHESFAAAEGAIGIARTANKVRTKPLQVILNGLGKDA
                     AYIISRINGFTYLETEYDYHTGNLAITKTKAFSEGDRAKVRCYGADDVNEGVAIMRHE
                     NVDVSITGNSTNPTRFQHPVAGTYKKWCIEHGKKYFSVA"
BASE COUNT          714 a          917 c          859 g          509 t
ORIGIN      
        1 gactagccgg gcggctgcgc cgatggtaga tactcccgtt cccgaatcgg cggtagcgga
       61 gccggacgaa cagggcgtcg acgagaccgg agcccccgta acctacaaga tcaccacgac
      121 caagtacaag gcctcggcga cctacgacac caacgtcctc ctcaacccgt cgacggacgt
      181 gatctatccc ggctccgtcc tgctcggcga gtcgatcgac gacggctcct acatcgagat
      241 caccggcggc gagaagcgcc cggtgaccat ctccttcgac ctctcgggag tggacggcga
      301 cgtcacgggc aagatcgtgc ccacgctatc gaactaccgc gagctgaaaa acaagatcct
      361 cggccagagc atgcccaggc agacctcgac ctattcgttc gagaagatag agatcaacag
      421 cgagtccgag ctcggcgtca aggtctcagc cggcgcctct ttcgacggcg gcgtgtatgc
      481 cgcgtcggtc aagaattcgt tcagcttcga caactcgaag aagacgaaca aggtcatggt
      541 caagttcatg cagaccttct atacggtcga cgtggaccag ggaaccttcc tctataaatc
      601 ctatgacctg gccgagttcg gcggttaccg ccccgtctac gtctcgtcga tagcctacgg
      661 ccgcctcgcc tacatcacca tcgaatccac cgagtccatc gaggccatca ataacaacct
      721 ggaggccgcg ttcaagtacg ggaacgccga ggcggaggcg agcgccgagg tagccaagag
      781 ctggctctct tcgaaggccc agaccaacat aacggtaata ggcggcagca cggtagcggt
      841 cgacctggag tccttcatgg cgatgctcca ggaggacgtc ttctcctcgg acaatcccgg
      901 taccatcatc gcctacaagc tccgtttcgt ggacgacaac accgtcgcca acaccatctt
      961 caacggcgac tacacgatac gcaaggccga ggccgtcaag ggcaagggca tcaaccttac
     1021 ccttaggatc accggcttga atgctcaggc ggacgatacc aacgggaatc ttgagctgta
     1081 cggcaggatc catttcaaat cgggcagcca gtccgaggat ctcctgaacg tagcgaatac
     1141 agactatatg cagctaccta agatttatac caactaccct gatctgagca cctgcgactg
     1201 gtggaaagga tccgccaaga cgctgaacct cgcgtcgacg actaccgatt tcaccatcga
     1261 gacgagcgcc ttcggcgagg tcgacgacgg ggcgaacgaa aaattcatcg acacagtaaa
     1321 ccctcttacg gtatcggctc ttacgaacgg caaagccttc aaaataaaat gcgacatggt
     1381 cggatacgta ggcaagcagt acctggagtt cacgatcgtg ccgaccatcg agtacctgta
     1441 ctaaggggcg gcggctgtcg cgcttccctg gcgatcggtg gtatactgac gcatattcac
     1501 cgaatcggag gaataagctg atgatgtata ccaccgaagt ggagcacatg tgcccgctcg
     1561 ccaagggctc gtaccacggt ccggctccca tacccgagga aggcaagtgg gtccaagcca
     1621 aggaaacgaa ggatatttca ggtttcacgc acggcgtcgg ctggtgcgcg ccgcagcagg
     1681 gcgcctgtaa gctctccctg aacatcaagg agggcgtcat ccaagaggcc ctcgtcgaga
     1741 cgataggctg ctcgggcatg acgcactccg ccgccatggc cgcggagata ctcataggca
     1801 agaccatcat cgaggcgatg aacaccgacc tcgtctgcga cgcgatcaac acggccatgc
     1861 gcgagctctt ccttcagatc gcctacggtc ggacgcagac cgcgttctcc gagggcgggc
     1921 tccccatcgg cgcgggactc gaggacctcg gcaagggtct gcgcagccag atcggcacga
     1981 tattcagcac gaaagcgaag ggcgtgcggt atctggaact cgccgagggc tacgtcaacc
     2041 agcttgcgct cgacacggat gaccagatta tcggctacga aatcgtccac ctgggcaaga
     2101 tgatggacat gatcaagaag ggaaccgatg cgaacgacgc gctgaaaaaa gcgacatcga
     2161 catatggccg tttcgacgat gcggccagga agatcgatcc tcgtcacgcg taacataagg
     2221 gtgattgcaa tggcactatt tgaaagctac gaaagacgaa tcaagcagat caacgccgtg
     2281 ctcggcgcgc atggcattac atcaattgag gaaaccaaat ccatctgcga tgcgaagggc
     2341 atcgatgtcg ccgcgatcgt gcggggtatc cagccgatat gctttgaaaa cgcctgctgg
     2401 gcctacacgg tcggcgccgc tatcgccata aaaaaggggg tgacggccgc ccccgaagcg
     2461 gcggaaatac tgggtcaagg cctgcaatcc ttctgcattc ccggttcggt cgccgacgac
     2521 cgcaaggtgg gcatcgggca cggaaacctc gcggcgatgc tcctgcgcga ggagacgaag
     2581 tgcttcgcgt ttctcgccgg gcacgaatca ttcgccgcgg cggagggcgc gatcggcatc
     2641 gccaggaccg ccaacaaggt gcgcaccaag cccctgcagg tcatcctcaa cggcctcggc
     2701 aaggacgcgg cgtacatcat ctcccgcatc aacggcttca cctacctcga gacggaatac
     2761 gattatcata ccggcaatct tgcgatcaca aagacgaagg ctttctccga gggcgatcgc
     2821 gcgaaggtgc gctgctacgg cgcggacgac gtgaacgaag gcgtcgccat catgcggcat
     2881 gagaacgtgg acgtctccat caccggtaac tccacgaacc cgacgcgctt ccagcacccg
     2941 gtcgcgggca cctacaagaa gtggtgcatc gagcacggca agaagtattt ctccgtggc
//
LOCUS       DAOPHV010000320         2999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_45769_length_2999_cov_2.923234, whole genome shotgun sequence.
ACCESSION   DAOPHV010000320 DAOPHV010000000
VERSION     DAOPHV010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45769_length_2999_cov_2.923234"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PLQ29_08730"
     CDS             <1..791
                     /locus_tag="PLQ29_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPG86771.1"
                     /translation="NILAIVLSAKVFGWKLGVARAVGAVAFSIILGLVMALIFRKDDE
                     KRAADARLFTVPDEKPARSLAQMIVYMASMIGILVFVNWANSKGGSAAWDAIYGAKYW
                     ITGAFGVVLAYTVMRWFNKEERVEWVVATRDFSLQILPLLLCGVLVAGFLLGRPGHDA
                     LIPSAWIAGLLGGNSLFANFVAALSGALMYFATLTEIPIIQGLLGAGMGQGPALALLL
                     AGPSLSLPSILVIGGELGWKKTLTYVTLVVILSTVAGMAFGAWA"
     gene            986..1525
                     /locus_tag="PLQ29_08735"
     CDS             986..1525
                     /locus_tag="PLQ29_08735"
                     /inference="COORDINATES: protein motif:HMM:NF022321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HPG86772.1"
                     /translation="MDEKKRPSFVGKLQPMAPSRRTLTIELLVIDMKTCARCVPTGAR
                     LAEAVRLLTPIAESLGIELVERMTLVGTPGEARRRALVSSPTIRINGRDIAQDIRESR
                     CESCGEIAGGGAAIDCREWHYKGTVYSAAPLSMLLEAIMRAMLDIDAIPMVEPRPLAE
                     LPDNLVRFFRGGASPSCCG"
     gene            1554..2009
                     /locus_tag="PLQ29_08740"
     CDS             1554..2009
                     /locus_tag="PLQ29_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86773.1"
                     /translation="MSEEINFAMIAGSLPRTDGILNLSPAEAYGFLRAGAMLVDLREP
                     YETNYRVFDVDEVLYVAWTSFLSGYAHVPRGRALILADASGIYCRAAARILTEAGYVN
                     VAKLSGGMIDWDAEGLPVRKDDGYELGGQCACKMKTRDGINPLMRKGDR"
     gene            complement(2151..2477)
                     /locus_tag="PLQ29_08745"
     CDS             complement(2151..2477)
                     /locus_tag="PLQ29_08745"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPG86774.1"
                     /translation="MTEGDKARLTRRATILKSLAHPSRLLIIEMLEQAPRCVGELTEA
                     VEADITTVSKHLALLRRTGLVRAEKRGTYSEYSLACDCISHLIDCIEDTHDGGLGAAE
                     DGLNER"
     gene            2596..>2999
                     /locus_tag="PLQ29_08750"
     CDS             2596..>2999
                     /locus_tag="PLQ29_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HPG86775.1"
                     /translation="MSDSQKAGLGAFAKYLSVWVALCIALGVAIGYFLPGVPAFLGRF
                     EYARVSIPVAILIWLMIYPMMLKVDFSSIKNAGKKPKGLIITLVVNWLIKPFTMYLIA
                     AFFLKIVFKALIPEALATEYLAGAVLLGAAPCT"
BASE COUNT          557 a          928 c          925 g          589 t
ORIGIN      
        1 tcaatatcct cgccatcgta ctgtcggcca aggtcttcgg ctggaagctc ggcgtcgcga
       61 gagcggtcgg tgccgtagcg ttttcaataa tactaggcct cgtcatggcg ctcatcttcc
      121 gcaaggatga cgagaagcgt gccgccgacg cccgcctgtt caccgtcccg gacgagaagc
      181 cggcccggag cctcgcccag atgatcgtct acatggccag catgataggc atcctcgtgt
      241 tcgtgaactg ggccaattcc aagggcggta gcgccgcctg ggacgcgata tatggcgcca
      301 agtactggat taccggagcc ttcggcgtcg tcctcgccta cacggtcatg cggtggttca
      361 ataaagaaga gcgcgtggaa tgggtggtcg cgacgcgcga cttctcgctg cagatcctcc
      421 ccctgctgtt gtgcggcgtg ctcgtcgctg gcttcctgct cggtcgaccg ggacacgacg
      481 cgctcattcc gagcgcctgg atagccggcc tcctgggcgg caactcgctg ttcgccaact
      541 tcgtcgcggc cctgtccggc gctctcatgt acttcgcgac gcttaccgag atacccataa
      601 tccagggcct cctgggcgcg ggcatgggcc agggaccggc gctcgcgctc ctactcgccg
      661 ggccgagcct gtcgctgccg tccatcctgg tcataggagg ggaactcggc tggaagaaga
      721 cgctgaccta cgtgaccctc gtcgtgatct tgtcgactgt cgccggcatg gcgttcggag
      781 cctgggctta agccggaaat taggagcaac cttatgaaga gagtatcccg cgtgaagggc
      841 ggcgtcacgc catgatcgat tccatcgtcg cctcgataag cgcggccttc ggaacgccct
      901 cggtgatcgc cttcggggcc gcagcgctgt aggcgtcgcg cagatgctag gctgaatcgt
      961 tggcatacag aagggggagc gaaaggtgga tgagaagaaa aggccctcgt tcgtaggaaa
     1021 gctccaaccc atggcgccgt cgcgccgcac gctgaccata gaactgctcg tgatagacat
     1081 gaaaacctgt gcccgttgcg taccgacggg cgcgcggctc gccgaggcgg tgcggctact
     1141 gactccgata gccgagtcgc tcggcatcga actggtcgag aggatgaccc tcgtcgggac
     1201 gcccggggaa gccaggcggc gcgctctcgt atcttcgccc accatacgaa taaacggtcg
     1261 tgacatcgcc caggatattc gggagtcgcg ctgcgagtcg tgcggggaga tagccggggg
     1321 cggagcggcg attgactgcc gcgagtggca ctacaagggt acggtctact cggccgcccc
     1381 actctcgatg ctcctcgagg ccatcatgag ggccatgctc gatatcgacg ccatacccat
     1441 ggtggagccg cggccgctag ccgagctgcc ggacaacctg gtgcgcttct tccgcggagg
     1501 cgcgtcgcca tcatgctgcg ggtaggcgga acatgaccga ccgggagatg ccgatgtcag
     1561 aagaaataaa ctttgccatg atcgccggga gcctgccgag gaccgatggg atcctcaacc
     1621 tgagccccgc cgaggcctat ggattcctgc gcgccggcgc gatgctcgtg gatctacgag
     1681 aaccatacga gaccaactac cgcgtgttcg acgtcgacga ggtgctgtac gtcgcctgga
     1741 cgagctttct ctcgggttat gcgcatgtgc cgcgcggccg agccctgata ctcgccgacg
     1801 cgtccggcat ctactgccga gccgccgccc gcatcctgac cgaggccggc tacgtcaatg
     1861 tggccaagct atccggcggc atgatcgact gggacgccga aggcctaccc gtgcgcaagg
     1921 acgacggcta cgagctgggc ggccagtgcg cgtgcaagat gaagacccgc gatggaatca
     1981 acccgctgat gaggaaaggt gatcgctaag ccgcgctcgc tctcgtttcg cagcgccttt
     2041 ccagcgtttg cagcggcgcg ccgcgacgct gtacttcgta tctcgttcct cgaactatcc
     2101 ctgatatagg aaaagccgga aagctataga agacggtccg aatctccggc tcatcgttcg
     2161 ttcagcccgt cctcggccgc cccgagtccg ccgtcatggg tatcctcgat gcagtcgatc
     2221 agatggctga tgcagtcgca cgcgagcgag tattcgctat aggtgcctcg cttctcggcg
     2281 cgtacgaggc ccgtgcgcct gagtagcgcc aggtgcttcg atacggtcgt tatatcggcc
     2341 tctacggcct cggtgagctc cccgacgcat ctcggagcct gttcgagcat ctcgatgatg
     2401 agtagcctcg acggatgggc cagggattta aggatcgtag ccctgcgggt cagtctcgcc
     2461 ttgtcgcctt cggtcatcgg tcctcccgac ctatccagct atggtatcat cgataactct
     2521 tgacattgtt ggccaatacg ccaataataa tacgtgtagt ggaactccgt gtcaacgata
     2581 caacgtggag gatacatgag cgacagccaa aaagcggggc ttggagcctt cgcgaagtac
     2641 ctatccgtct gggtagcact ttgtatagcg ctcggcgtgg ccataggata cttcctgccg
     2701 ggcgtgccag cgttcctggg ccgcttcgag tacgcgagag tctcgatacc ggtggcgata
     2761 ctcatctggc tcatgatcta ccccatgatg ctcaaggtcg acttttcaag catcaagaat
     2821 gccgggaaaa agcccaaggg gctcatcatc accctggtcg tgaactggct cataaagccg
     2881 ttcacaatgt accttatagc cgcattcttc ctcaagatcg tcttcaaggc gctcatcccg
     2941 gaagcgctcg ccaccgagta cctggcgggc gccgtgctgc tcggtgcggc gccgtgcac
//
LOCUS       DAOPHV010000321         2990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_45952_length_2990_cov_4.402385, whole genome shotgun sequence.
ACCESSION   DAOPHV010000321 DAOPHV010000000
VERSION     DAOPHV010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45952_length_2990_cov_4.402385"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLQ29_08755"
     CDS             complement(<1..609)
                     /locus_tag="PLQ29_08755"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPG86776.1"
                     /translation="MLLALDARNSGIVAGFRDGDGWKSIVRLGVDRSADEIGILLEAA
                     AARAGVGTTTASVDEAWLSCVVPALTSRLVHAVREAFGIDASTVGPGTRTGLKIRTDN
                     PSELGSDIVCSCVAARCVAARCVAAREITSREAESPAVIVVDFEAALVVSAVNADGEL
                     LGVSIAPGLETAARSLRASAAQIPEIRLDVPRRAIGKNTAQSL"
     gene            complement(613..1221)
                     /locus_tag="PLQ29_08760"
     CDS             complement(613..1221)
                     /locus_tag="PLQ29_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPG86777.1"
                     /translation="MERSNVRKIVVAGALGAVSAVMAVTPLGYLPWFGGASLTIMHIP
                     VIIAAVLEGPLAGTAVGLVFGVTSLVRAATAPVGPLDPLFANPIVSVLPRLFIAIAAW
                     AAFKLFRGKASPVAAGFAGAVGSLVNTALVLSALGLVGAEQLSGLFGVEPGALPAALA
                     ALALSNGLVEAAAAAVFTSSVVSAWKGIESARGKARLSEEER"
     gene            complement(1311..2159)
                     /locus_tag="PLQ29_08765"
     CDS             complement(1311..2159)
                     /locus_tag="PLQ29_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HPG86778.1"
                     /translation="MKGLEFWKHVSIGRYVESDSMVHRMSPSTKYLWLLAAVLPASVS
                     RSAIVVAAVTLLPLILAPLAGVRIRFLLRGVLPVLPFVALAAAIQLLIAWPNDASPVL
                     ARVGPVSVTAREALAVLTMGLRFIAIMTAIGLFTSVTSEGDTARGVEDLFSPLSSLGF
                     PARPLALMIAVAFRFIPIIAGEFESVVKAQAARGADFGAGARGPIRKAKAYLPLLVPV
                     TIRALERAEALAEAMEARCYGTGPATRYVDTAGGAADVVARIVAMLWILAALAGPALA
                     ASVGGS"
     gene            complement(2156..>2990)
                     /locus_tag="PLQ29_08770"
     CDS             complement(2156..>2990)
                     /locus_tag="PLQ29_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG86779.1"
                     /translation="TGSATTDSATTDSGGASAFSLRGASMRYLAGTESERLAVDAATL
                     ELPSGAITALIGRTGSGKSSLLQLLDALALPDSGIVVSLGRVTAEPGTDLRAVRMRAP
                     LAVQRPESALFELYAGDEVAFGPRNQGLSGKALVERCRAAMDEAGLPFDEYRDALSRA
                     LSGGRKRRLAIASVLSLDPEALLLDEPTSALDPASRDEVMRLIRSYADRGRTVVMSTH
                     SMDEAARADMVAVMRGGAVVAFGPPGQVFGEGWDPAWGVGRPFAYELLEAVSRDGEAT
                     Q"
BASE COUNT          507 a         1062 c         1036 g          385 t
ORIGIN      
        1 gagcgactgg gcggtgttct tgcctatggc ccggcgcggc acgtcgagcc tgatctcggg
       61 gatctgggcg gccgaggcgc ggagcgaacg ggcggccgtc tcgaggccgg gagcgatcga
      121 tacgccgagt agctcgccgt cggcgttgac cgccgacacg acgagggccg cctcgaagtc
      181 gacgacgatg accgcgggcg actccgcttc gcgcgacgta atctcccgtg cggccacgca
      241 ccgtgcggcc acgcaccgtg cggccacgca cgagcagact atatcgctac caagttccga
      301 ggggttatcg gtgcggatct tgagccccgt cctggtgcct ggcccgaccg tcgaggcgtc
      361 tatcccgaag gcctcgcgga ccgcgtggac gaggcgggag gtaagcgccg ggaccacgca
      421 cgacagccag gcctcgtcga cggaagccgt cgtcgttcct acgccggcgc gggccgcagc
      481 ggcctcgagc agtatgccta tctcatcggc gctacggtcg acgccgagtc gcacgatgga
      541 cttccagccg tctccatccc tgaacccggc gacgatgcca gaattcctcg cgtcgagcgc
      601 caacagcacg gctcagcgct cctcttcgga cagccgggcc ttgccccgag cgctctcgat
      661 gcccttccag gcgctgacca ccgagctcgt gaacacggcg gcggcggcgg cctcgacgag
      721 gccgttggat agggcgaggg cggcgagggc agcgggcagg gcgccaggct cgacgccgaa
      781 caggccggat agctgttccg cgccgacaag gccgagggcc gatagcacga gggccgtgtt
      841 gacgagacta ccgaccgcgc cggcgaagcc cgcggctacc ggcgacgcct tgcccctaaa
      901 cagcttgaag gctgcccagg ccgctatcgc gatgaacagg cgcggcagca ccgacacgat
      961 cgggttggcg aacaaggggt cgagcgggcc gacgggagcg gttgccgcgc gcacgaggct
     1021 cgtcacgcca aagaccaacc cgacggccgt accggcgagc ggtccctcga gcacggccgc
     1081 gatgatgacc gggatatgca tgatggtcag cgaagccccg ccgaaccacg gcaggtagcc
     1141 gagcggcgtc accgccatga cagccgagac cgcgccgagc gccccggcga ccacgatctt
     1201 ccgtacgttc gatcgttcca ttcatcttcc tccgttcggg gtacgcgccg cctcggacgg
     1261 tcgcgtccca tggctatgag cgcggccctg gccgcgcgcg aagaaccgta ctaggagcct
     1321 ccgacggagg cggcgagcgc cggccccgcc aaggcggcga gtatccatag cattgcgacg
     1381 atgcgggcga cgacgtcggc ggcccctccg gccgtatcga cgtaacgcgt cgccggcccc
     1441 gtgccgtagc agcgcgcctc catcgcctcg gcgagggcct cggcccgctc gagcgcccgg
     1501 atggtcaccg gcacgaggag gggcaggtac gccttggcct tacgtatcgg cccccgagcg
     1561 ccggcgccga aatccgcgcc cctagccgcc tgcgccttga cgaccgactc gaattcaccg
     1621 gctatgatcg gtatgaacct gaaggccacg gcgatcatca gcgcgagcgg cctggccggg
     1681 aagccgaggg atgataacgg ggaaaacagg tcctcgacgc cgcgcgccgt atccccctcg
     1741 ctggtcaccg aggtgaacag gccgatcgcc gtcatgatcg cgatgaagcg caggcccatc
     1801 gtcagcacag ccagggcctc ccgcgccgtg accgataccg gccctacccg cgccagcacg
     1861 gggctggcgt cgttcggcca ggcgatgagc agctggatgg cagcggccag cgcgacgaag
     1921 ggcagcacgg gcaagacgcc gcgcaagagg aagcgtatcc ggaccccggc aagcggcgcc
     1981 aggatcaacg gtagcagcgt caccgcggcg acgacgatcg ccgagcggga tacgctcgcc
     2041 ggaagcaccg ccgccaagag ccacaggtac ttcgtcgacg gcgacatgcg atggaccatc
     2101 gagtccgact cgacgtagcg gccgatggat acgtgcttcc agaattccag gcccttcatt
     2161 gagtagcctc cccatcccgc gagacggcct cgagcaactc gtaggcgaag gggcgcccga
     2221 cgccccaggc cggatcccag ccttcgccga atacctgccc aggcggcccg aacgcgacga
     2281 cggcgccgcc ccgcatcacc gcgaccatgt cggcgcgtgc cgcctcgtcc atcgaatgag
     2341 tagacatgac gacggtccgg ccgcgatcgg cgtaggaccg tatcagacgc attacctcgt
     2401 ctcgcgacgc cgggtcgagg gccgaggtcg gctcgtcgag gagtagcgcc tcggggtcga
     2461 gcgacagcac cgacgctatc gccaggcgac gcttgcggcc gcccgacagg gcgcgcgaca
     2521 gcgcgtcccg atactcgtcg aacggcaggc ccgcttcgtc catcgctgcc ctgcaacgtt
     2581 ctaccagggc tttgccggac agcccctggt tgcggggccc gaaggccacc tcgtcgccgg
     2641 cgtacagctc gaacagggcg ctctcggggc gctggacggc gagcggcgcg cgcatacgca
     2701 cggcccgcag gtcggtaccg ggctccgcgg tcacccggcc gagcgatacg acgatgcccg
     2761 agtcgggcag ggccaacgcg tcgaggagct ggagcaggct cgatttgccc gaaccggttc
     2821 gaccgatgag cgcggtgatc gccccggacg gtagctcgag cgtcgcggcg tcgacggcga
     2881 gccgttcgct ctcggtgccc gccaggtagc gcatcgacgc gccccggagc gagaaagccg
     2941 aagcgccgcc cgagtccgtg gttgccgagt ccgtggttgc cgagcccgtg
//
LOCUS       DAOPHV010000322         2985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_46055_length_2985_cov_4.660410, whole genome shotgun sequence.
ACCESSION   DAOPHV010000322 DAOPHV010000000
VERSION     DAOPHV010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46055_length_2985_cov_4.660410"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLQ29_08775"
     CDS             complement(<1..289)
                     /locus_tag="PLQ29_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPG86780.1"
                     /translation="MRLVDPECAAIAAAFSRRDLRARLPESIAAKDPMYRRSGSSYDL
                     KSIREYQPFDDPRAIDWKLYGRSDRAFVKEFYDEADDEVAFLVDSSASLSCA"
     gene            complement(286..1293)
                     /locus_tag="PLQ29_08780"
     CDS             complement(286..1293)
                     /locus_tag="PLQ29_08780"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG86781.1"
                     /translation="MQPDEFERSLASIKDLYDDYTAFVGTRVLGYPRILERLFIAFLS
                     GGNVLLEGAPGLGKTTLAKTWSEFFGLGFSRVQFTPDLMPLDIIGSNLLEEGPSGRSF
                     RFYRGPIFSNVVLGDEINRATPKTQSAFLEAMEEKRVTFLGEVHPLPDPFFVIATQNP
                     IELEGTYPLPEAQIDRFFMKLNFAMPDLHALLGIMDLQATEKHGRAIGAEASQEAVAG
                     WRALAPHIAAPEGVRAYIARIVEHSHPDRSPVELVRKYVLYGASPRSAIALLAAARTK
                     AAMAGRPSVSFDDVDELLADVAAHRLILNFEAEADGVTVADVLGAVRADAAKAFARER
                     R"
     gene            complement(1280..1906)
                     /locus_tag="PLQ29_08785"
     CDS             complement(1280..1906)
                     /locus_tag="PLQ29_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86782.1"
                     /translation="MRRASILAASATTLRTLAAIAAGAALWYAMRLCQGALRPVLGRV
                     PEWAALAANAGVEEALRLGLALLIARYVRRAGLEPGTVGLGVVAACSLGALENASYLA
                     AFPTLDAYWRLGYAVPIHAGAAALYAIGTGESKSPDGASRPTSWPAGIAAAFTLAWAW
                     HSGFNIVAALFPFPALPIVGTVVNLIALSALVVALAIRYGYWSVYAAR"
     gene            complement(1908..>2985)
                     /locus_tag="PLQ29_08790"
     CDS             complement(1908..>2985)
                     /locus_tag="PLQ29_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86783.1"
                     /translation="RFRSVAWLGTAFAAWSLTLLASVSLLDTRLMPSPLAFNAIVMAI
                     VGSLPAFLAYPARRSWGMDAIRAADSETSIEAWLGYRGGPAERLLERRALEALGLATI
                     GVPQATRRPASARRFVAALYAVGLASFVAAQIASIGSGCGLSLGYPEREIVPAVSDAS
                     VAIEDGYPAVVEAEAEEVDEKPAEDGSAEPRPSRSAGEADENRLEEPDFVAASDGRPG
                     DGAAEAAATDQAKAPSRSAVAGAGTRRGGAVSSGDGSGDGSGGEASSPGYEGRGRAIE
                     PSPMLDYRARFERQYAEATGVETAIGPEPSAETVSAAITELFSSYDARVVVGGDVDPE
                     LAGLGAAWLDAFGPGAAATGERRP"
BASE COUNT          455 a         1017 c         1065 g          448 t
ORIGIN      
        1 gggcgcacga caggctggcc gaagagtcga cgaggaaggc cacctcgtcg tccgcctcgt
       61 cgtagaattc cttgacgaaa gcgcggtcgg atcggccgta tagcttccag tcgatggccc
      121 gcgggtcgtc gaaaggctgg tactcacgga tgctcttgag gtcgtagctc gagccggagc
      181 ggcggtacat ggggtccttg gccgcgatcg actcgggtag cctggcgcgc aggtcgcgcc
      241 gggagaaggc cgccgcgatg gccgcgcact ccgggtcgac gagcctcatc ggcgctccct
      301 ggcgaaggcc ttggcggcgt cggccctgac cgctccgagc acgtcggcca ccgtgacgcc
      361 gtcggcctct gcctcgaagt tgaggatcag gcggtgggcg gccacgtcgg cgagcagttc
      421 gtcgacgtcg tcgaacgaaa cgctcgggcg gccggccatc gcggccttgg tcctggccgc
      481 cgcgaggagg gctatggccg acctcggcga ggcgccatac aggacgtact tccgcaccag
      541 ctcgaccggc gagcggtcgg ggtggctgtg ctcgacgatg cgggctatgt aggcgcgtac
      601 gccttcgggc gcggctatat ggggcgccag ggcccgccag cccgcgacgg cctcctgcga
      661 ggcctcggcg ccgatggccc ggccatgctt ctcggtcgcc tggagatcca tgatgccgag
      721 cagggcgtgg aggtcgggca tcgcgaagtt gagcttcatg aagaaacggt ctatctgcgc
      781 ctcggggagc gggtaggtgc cctcgagctc gatggggttc tgcgtcgcta tgacgaagaa
      841 gggatcgggc aggggatgga cctcgccgag gaaggtgacg cgcttttcct ccatcgcttc
      901 gaggaaggcc gactgggtct tgggggtcgc ccggtttatc tcgtcgccga gcaccacgtt
      961 ggagaagatg ggccctcggt agaagcggaa cgaacgcccg gaggggcctt cctcgagcag
     1021 gttcgagccg atgatgtcga gcggcatcag gtccggggtg aactggaccc gactgaagcc
     1081 gagcccgaag aactccgacc aggtcttggc cagcgtcgtc ttgccgagtc caggagctcc
     1141 ctcgagcagg acgttgccgc cggataggaa cgctatgaac aggcgctcga gtatcctcgg
     1201 gtagccgagc acgcgggtgc cgacgaaggc cgtgtagtcg tcgtagaggt ccttgatcga
     1261 ggcgagcgat cgttcgaatt catcgggctg catacacgct ccagtatccg taccgaattg
     1321 ccagcgccac gacgagcgcc gagagggcta tcaggttgac gaccgtgccg acgatcggca
     1381 gggccgggaa ggggaacagg gccgcgacta tattgaagcc tgaatgccag gcccacgcga
     1441 gcgtgaaggc cgcagctatc ccggccggcc agctcgtcgg acggctcgcg ccatcggggc
     1501 ttttgctctc cccggtgcct atggcgtaca gcgccgcagc cccggcgtgg atcggcacgg
     1561 cgtagcccag acgccagtac gcgtcgagcg tcgggaaggc ggccaggtac gaggcgttct
     1621 cgagcgcgcc gagcgagcag gccgcaacga cgccgaggcc gaccgtaccc ggttcgagcc
     1681 cggcccggcg tacgtatcgc gctatcagca aggccaggcc gagcctgagc gcctcctcga
     1741 cgccggcgtt ggcggctagc gcggcccatt ccggcacgcg cccgaggacg gggcgcagcg
     1801 ctccttggca caggcgcata gcgtaccaca gcgccgcgcc ggcggcgatc gccgcgagcg
     1861 tccggagcgt cgtggccgag gcggccagga tcgatgctcg cctcatgcta gggcctcctc
     1921 tcgccggttg cggctgcccc ggggccgaag gcgtcgagcc aggcggcccc gagtccggcc
     1981 agctccgggt cgacgtcccc gccgacgacc acccgggcgt cgtagctcga gaaaagctcg
     2041 gttatggcgg ccgagaccgt ctcggcgctc ggttccgggc ctatggccgt ctcgacgccg
     2101 gttgcctcgg catactggcg ctcgaagcgc gcgcgatagt ccagcatcgg gctcggctcg
     2161 atggcccgcc cccgcccctc gtagccgggg ctcgaggcct cgccgccaga accgtcgccc
     2221 gagccgtcgc ccgaggatac ggcgccgcct cgcctggtcc cggccccggc caccgccgaa
     2281 cggctcggtg ccttggcctg atccgtcgcc gccgcctcgg ccgctccgtc gcccggtcgg
     2341 ccgtcggacg ccgccacgaa gtcgggctcc tcgagcctgt tctcgtcggc ttccccggcg
     2401 cttcgggatg ggcgaggttc ggccgatccg tcctcggcag gcttttcgtc gacttcctcg
     2461 gcctcggcct cgacgacggc tggatacccg tcttcgatag cgaccgacgc gtccgatacg
     2521 gccgggacta tctcgcgttc cggataaccg agggataagc cgcagcccga gcctatcgac
     2581 gcgatctggg cggccacgaa gctggcgagt ccgacggcgt acagcgccgc tacgaaacgg
     2641 cgcgcgctcg ccggacgcct cgtcgcctgc ggcactccta tcgtcgcgag gcccagcgct
     2701 tcgagggccc tccgttcgag cagccgctcg gcggggccac cccgatagcc gagccaggct
     2761 tcgatcgagg tctcgctatc ggccgcccgt atggcgtcca tgccccagga tcgcctcgcc
     2821 ggataggcca ggaaggccgg gagcgagccg acgatcgcca tcacgatggc gttgaacgcc
     2881 aatggggacg gcatgagcct cgtgtcgagc aggctgaccg acgccagcag cgtcagcgac
     2941 caggcggcga aagcggttcc gagccaggcg accgagcgga accgt
//
LOCUS       DAOPHV010000323         2982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_46138_length_2982_cov_3.673727, whole genome shotgun sequence.
ACCESSION   DAOPHV010000323 DAOPHV010000000
VERSION     DAOPHV010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46138_length_2982_cov_3.673727"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..771)
                     /locus_tag="PLQ29_08795"
     CDS             complement(310..771)
                     /locus_tag="PLQ29_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86784.1"
                     /translation="MRIRRIAGMACIAFGALNIVNFMAPIPVPTVGPGAFIVGFAFIG
                     FGLYLRLSQGGSGSGQGTRIGGTPKSTIGATVDGARGTTDPLLSVRALRLASESKGIL
                     TVSKTAMRLNVPLDEATAALDECALKGAAYIIVDDSTGVASYCFPEFMPKE"
     gene            complement(892..975)
                     /locus_tag="PLQ29_08800"
     tRNA            complement(892..975)
                     /locus_tag="PLQ29_08800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(939..941),aa:Leu,seq:taa)
     gene            1194..1562
                     /locus_tag="PLQ29_08805"
     CDS             1194..1562
                     /locus_tag="PLQ29_08805"
                     /inference="COORDINATES: protein motif:HMM:NF018228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPG86785.1"
                     /translation="MKTLLIIDDDALIRDTVSMMLDRAGYKGLCAASSEEGMAMMASN
                     QVALALVDVVLPDRNGLELAFELNQKWPSVPIVLMSGRVSTEADSIKNFNGHFGIKGS
                     IPKPFTLEQLLAAVRGALGE"
     gene            1701..2864
                     /locus_tag="PLQ29_08810"
                     /pseudo
     CDS             1701..2864
                     /locus_tag="PLQ29_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
BASE COUNT          533 a          985 c          962 g          502 t
ORIGIN      
        1 cagcagccgg cccgaaggag ccaggcgggt caagacggcc agcgtggacc gaggaccgac
       61 gaacgccgcg acggctccgg gaatcgccag ggcggcccgg cctcgaccag gcccgccccc
      121 aggcgcgatg ccccccggta cgagccgcct gtgcgctcgt ctcgggacgt gcgctctgtc
      181 atcgacgaga tcacgggccg gcgatcgtag gcgccgccgc gcttagcgcg aaggatatgg
      241 aacggccgcc cgcgctatgt ggggcggccg ttctcgttag tgtgcgatgc ccttcggacg
      301 ggctcctcgt tattctttgg gcatgaactc agggaaacag tagctcgcga cgcccgtcga
      361 gtcgtcgacg atgatgtacg ccgcgccctt gagcgcgcac tcgtcgagag ccgccgtggc
      421 ttcgtccaac ggtacgttga gcctcatggc ggtctttgac accgtcagta tccccttgga
      481 ctcggaggcg agccgtaagg ctcgtacgga caggagtggg tcggtcgttc cgcgcgcgcc
      541 atccaccgtt gccccgatag tcgacttggg cgttccgccg atccgcgtac cttgacccga
      601 tccggaaccc ccttgggaca agcgtaggta caggccgaac ccgatgaacg cgaagccaac
      661 tatgaaagcg ccgggcccga ctgtcggcac cgggatgggc gccatgaaat tgactatatt
      721 cagggctccg aaggcgatgc acgccatgcc ggctattctt ctgatcctca tgccgtcccc
      781 ctccaggcca tgctccaggg cgtcagcgag cctaggacga tactacaggg cctccggacc
      841 agacgcaaga ggagtcgaac gccagacggg gtacattacc gaggagcgta gtgccgggaa
      901 cgggacttga acccgtacgc ccttgcggac aagggatttt aagtccctgg tgtctaccga
      961 ttccaccatc ccggctcgtg ctgcggcttc cggcggcatg atagccgtag cccgtcgcgg
     1021 cgtcaaggta tggagaatag ggcggcctcg agcgaactgc ccctcgggcg atcgctccgg
     1081 gccgtagcct cggatgcggc ttgatcccga gcccgcgtag gcgtatagtg gccagctatc
     1141 gacgtgcgta cacgtatcgt tagatttcca gaaaaacgcc agaggaaacg ctagtgaaaa
     1201 ccctgttgat catcgacgac gacgccctga tccgcgatac ggtatcgatg atgctggatc
     1261 gcgccggcta caagggcctg tgcgccgcct cctcggagga aggcatggcg atgatggcct
     1321 cgaaccaggt cgccctggcc ctcgtcgacg tggtactgcc cgacaggaac ggcctcgagc
     1381 tagccttcga gttgaaccag aaatggccat cggtgccgat cgtgctgatg tccggccgcg
     1441 tctcgaccga ggctgactcg atcaagaact tcaacgggca tttcggcatc aagggctcca
     1501 tccccaagcc gttcacgttg gagcagctcc tcgcggcagt ccggggcgcg ctcggcgaat
     1561 agccgggccc ggctaattct tccttgacgt cccggggcgg cgggtctacc ctgacctgat
     1621 cctggcccgc cttggctggc ccgcgttccg gggggactat gcttataaaa atactgtggt
     1681 accggctctt ccaggctatc atgcgcctcg cggcgaactt cctcgacttt acgccgcccg
     1741 agctactcga cggccccggc agcgtcaagc gtcttccgtc gttcatcaag tcaaagggct
     1801 tctcgagggt gctcgtcgtc accgacaagg gcctcatggc ggcgcgcctg ctcgacgggc
     1861 tgttcgacag cctccgcgag gccggcgtcg agtacgcgct gtttgacggc gcccaggcca
     1921 acccgaccat ccaaaatatc gaggacgcgc tgacgttata ctaggaaaag ggttgccagg
     1981 cgattatagc cttcggcggc ggctcgtcga tggattgcgc caagggctgc gccgccaggg
     2041 ttaccaaccc cggcaagacg atcccgagga tgcgcggcct cctcaaggtc gggcgtaagc
     2101 cggcgaccgt cttcgccgtg ccgacgaccg ccggcaccgg ttccgagacg accatcgcgg
     2161 cggtcataac cgatccttcg acgcacgaga agtacgccat caacgatccc aagctgctac
     2221 cgccctacgc ggtgctcgac cccgagctga ccgtcggcct gccgccccat atcacctcga
     2281 cgacgggcat ggacgcgctg acgcacgccg tcgaggccta cgtcggccgt agcaacacga
     2341 agaacacgat cgagaaggcc gagaaggccg tagcgctgat ctacgcgaac ctcgagacgg
     2401 cgtataagga cggcaagaac gtcggggcgc gagccaacat gctgctcgcc tcgtactacg
     2461 cgggcatcgc gttcaccagg gcctacgtcg gctacgtgca cgcgatagcg cacaatctcg
     2521 gcgggctgta cggcctgccg cacggtctgg ccaacgcggt gatcctgccg tacgtgctcg
     2581 actacttcgg cgagtcggcc cacgcgccgc tcgcgaggct ggccgacatc gcgggcgtct
     2641 cgtcggcggg gcagagcgtc accgagaagg ccgaggcctt cgtcgcctcg atccgcgggc
     2701 tcaaccggcg catggctatc cccgagggct tcgagcagat ccgcgaggag gatataccgc
     2761 tgatcgtcga gcgggcgttc cgggaggcgc acccgctgta cccggtcccg aggatcatga
     2821 gccgcgccga gtgcgaggct ctcgtccgca ggctgatgcg ctaatcgagg ccggctcggt
     2881 gcgagccgaa gagccccctt ccggcgagcc gggagggggc tttcctatgc gtcgttgcgg
     2941 tcctcgacca tctcagaggt cgagtagaag cgtatcgcgg ga
//
LOCUS       DAOPHV010000324         2971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_46373_length_2971_cov_4.573388, whole genome shotgun sequence.
ACCESSION   DAOPHV010000324 DAOPHV010000000
VERSION     DAOPHV010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46373_length_2971_cov_4.573388"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..767
                     /locus_tag="PLQ29_08815"
     CDS             258..767
                     /locus_tag="PLQ29_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86786.1"
                     /translation="MQAPAEPEPLSESPAVRPLPEPIEPSPEPTGIESPAPFDPDSVS
                     VEMKQATFFDVRALIQGLNSIIQAKDYESWAMALTDDYRSYYSSSDTLDRISDAPVLK
                     RQGVKLRSLQDYFIYVVYPSRQNDRVDDIEFIDATHIRAITVNVKGERLVLYNLEKIG
                     DTWKIAIWR"
     gene            770..1492
                     /locus_tag="PLQ29_08820"
     CDS             770..1492
                     /locus_tag="PLQ29_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPG86787.1"
                     /translation="MKRSICAFVVLAVLASVPVWSQTASSGERTVEESYLQDPLETMI
                     IREQAYSDSKDMKLVALQYIRQALEEGRASPEVRKALEYLALESSTTVIRAGGVGRVL
                     NNYPDVRREACLMLGEIKTVEAKDALLKVALADNEPMVIAAALRSIGKIGIMDGDDVT
                     QTIAFVVNRFDILFPDNSLAFESLVALEALAEANGGIKDPAAIRAIMKIADGSYITPV
                     RQKAKDLLTKLRKYSGSSGSGK"
     gene            1608..1763
                     /locus_tag="PLQ29_08825"
     CDS             1608..1763
                     /locus_tag="PLQ29_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86788.1"
                     /translation="MDVDGVVPSLPPRPLPDRGLPTERPPEPEPVPEPEQPPSDEGNG
                     TMLDLYA"
     gene            complement(1840..1913)
                     /locus_tag="PLQ29_08830"
     tRNA            complement(1840..1913)
                     /locus_tag="PLQ29_08830"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1877..1879),aa:Arg,seq:ccg)
     gene            2015..2596
                     /locus_tag="PLQ29_08835"
     CDS             2015..2596
                     /locus_tag="PLQ29_08835"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPG86789.1"
                     /translation="MEPLLLASSSPRRIETLRGYGFPLVVSPADVDESSYDALPVGER
                     VQALAELKVRVGAAAAPEPPFWALGADTLVALDGEAYGKPADEAEARAMLRSLSGRTH
                     QVLSGLCALDRRTGRVEKAISETAVTFSALGERELDWYIASGEWRGVAGAYRIQERAA
                     FFVERIDGSYSGVVGLPLHAFYAILSRIGYPIP"
     gene            2746..>2971
                     /gene="rpsB"
                     /locus_tag="PLQ29_08840"
     CDS             2746..>2971
                     /gene="rpsB"
                     /locus_tag="PLQ29_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPG86790.1"
                     /translation="MAVVTMKSLLESGVHFGHQVKRWDPRMKKFIFAERNGIHIIDLQ
                     KTIQSIKEAYDVVRKTVASGKPVLFVGTKKQ"
BASE COUNT          571 a          933 c          923 g          544 t
ORIGIN      
        1 acgctgccgt tgagctggtt gttggtgcgc agccaccagc ggaagcggtc caggagggcg
       61 cgatcgacct cccccggggc cttccagaag tcatcgaggc tgccctggaa ggtgagcccc
      121 ggcatgtcat agacggccgt ccccatgcac ttgaccgcag tgcgatggtg cagggacgac
      181 atcccaaccg tgctgttgat gacgatggtc cccagggcat tgcgcagcgt cgccgggaga
      241 ttgacgacgt cgacgtaatg caggcgcccg cggagcccga gcctctcagc gaatcgcccg
      301 ccgtcaggcc gttgcccgag cccatcgaac cgtcccccga gcctaccgga atcgagtcgc
      361 cggctccgtt cgatcccgac tcggtcagcg tcgagatgaa gcaggccacc ttcttcgacg
      421 tgcgcgccct tatccagggg ctcaactcca tcatccaggc gaaggactac gaatcgtggg
      481 ccatggccct gaccgacgac tatcggtcgt actactcatc atccgatacc ctggatagga
      541 tatcggacgc gccggtactg aagcgccagg gcgtcaagct gaggagcctt caggattatt
      601 ttatctacgt agtctatcct tcgaggcaga acgacagggt cgacgatatt gagttcatag
      661 acgccaccca tatccgcgcc atcaccgtca acgtcaaggg cgagcgcctg gtactttaca
      721 accttgaaaa aatcggcgat acatggaaga tcgcgatctg gaggtaatca tgaagcgtag
      781 catttgcgcg ttcgtcgttt tggccgtgct agcctcggtc cccgtatgga gccagacggc
      841 ctcgagcggg gagcgcaccg tcgaggagtc gtacctgcag gatccgctcg agacgatgat
      901 catccgcgag caggcctact ccgacagcaa ggacatgaag ctcgtggcgc ttcagtacat
      961 ccgccaggcg ctcgaggagg gtcgagccag tcccgaggtc cgcaaggccc tcgagtacct
     1021 ggccctcgag agttccacca ccgtgatacg ggccgggggc gtcggccgcg tcctcaataa
     1081 ctatcccgac gtgcgacgcg aggcctgcct catgctcggc gagatcaaga ccgtcgaggc
     1141 caaggacgcg ctcctcaagg tcgcgctggc cgataacgag cccatggtca tagctgcggc
     1201 cctccgttcc atcggcaaga taggcatcat ggacggtgac gacgtgacgc agaccattgc
     1261 tttcgtggtc aaccgtttcg acatcctgtt ccccgacaac agcctggcct tcgagagcct
     1321 cgtcgcgctc gaggcgctcg ccgaggcgaa cggcggcatc aaggaccccg cggcgattcg
     1381 cgcgatcatg aagatcgccg acggttccta tattaccccg gtcaggcaga aggccaagga
     1441 tctgttaacg aagctcagaa agtactccgg ttcgtccggc tccggtaaat agtccggagc
     1501 ggcgcgaaaa ggtcggcgct cgattggcgc cggccttttt ttacctcgcc gcccgaaagc
     1561 ggtatattaa acttagacgc gaatgctatc gcgccgggga ggctatcatg gatgtggatg
     1621 gagtggttcc gagccttccg ccgaggcccc ttcccgatcg cggcctaccg acggaacggc
     1681 cgcccgaacc ggaaccggtt cccgagccgg agcaaccgcc gagcgacgaa ggcaacggca
     1741 cgatgctcga cctgtacgcc tagctatacg aaaagaccgc gacctacaac gctcgccgag
     1801 ggcttcgtcg taggtcgcgg tccgttcttc gcgcgttctt gagcgatgcc gggctcgaac
     1861 cggccacctt cagctccgga ggctgacgct ctatccatat gagctaatcg ctcgacaggc
     1921 taagtagtac gccagaacga gcaccattgt caaccttcca tcccgagtag ctttcgtgta
     1981 gcatcgcgtc atggtaactg gaacaggatg ctttttggag ccgctgcttc ttgccagctc
     2041 ctcgccgcgc aggatagaga cgctccgcgg ctacgggttc cctcttgtcg tatcgccggc
     2101 cgatgtggac gagtcgtcgt acgatgcgct accagtcggc gagcgcgtgc aggcgctcgc
     2161 cgagctgaag gtacgcgtcg gggccgccgc agcgccggaa ccgccgttct gggcgctcgg
     2221 cgccgatacg ctcgtagcgc tcgatggcga ggcgtacggc aagcccgctg acgaggccga
     2281 ggcccgagcc atgctacgct ccctctcggg caggactcat caggtgttgt caggcctctg
     2341 cgccctggac cggcggacgg ggcgggtcga gaaggcgatt tccgagaccg cggtcacctt
     2401 ctcggctctg ggcgagcgcg agctcgactg gtatatcgcc tcgggagagt ggcgcggcgt
     2461 cgcgggcgcc tatcgcatcc aggagcgagc cgcgttcttc gtcgagcgta tcgatggctc
     2521 gtattctggg gtcgtgggct tgccactcca tgcgttttat gccatcctat cgcggatagg
     2581 ctacccgatt ccgtaggaaa aagggcctgt ctcgtcgtcc cgggcgcacg cgccacaggc
     2641 cgatgaatct tccgtcgatc catggtcgcc tcgcgcggct atccatatcg acgagaacaa
     2701 gcgaaggggg ctcccgcccg aacgcggggg ccgatggagg aacacgtggc cgtagtcacc
     2761 atgaagagcc ttttggagtc cggagtccat ttcgggcacc aggtcaagcg ctgggatccg
     2821 cgcatgaaga aatttatctt cgccgagcgg aacggcatcc atatcatcga tctgcagaag
     2881 acgatccagt cgattaagga agcctatgac gtggtccgca agacggtcgc gtccggcaag
     2941 ccggtgttgt tcgtcggcac caagaaacag g
//
LOCUS       DAOPHV010000325         2968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_46437_length_2968_cov_4.840027, whole genome shotgun sequence.
ACCESSION   DAOPHV010000325 DAOPHV010000000
VERSION     DAOPHV010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46437_length_2968_cov_4.840027"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PLQ29_08845"
     CDS             complement(<1..540)
                     /locus_tag="PLQ29_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HPG86791.1"
                     /translation="MKDLKLTGVRPDGARTVVEVGGIRIGDGFTVIAGPCAVESEDQL
                     LRTAVAIKEAGATMLRGGAFKPRTSPYAFQGLGLKGLKILDKARRETGLPIVTEVIDT
                     RDVSWVGEYADVLQIGARNMQNFSLLREAGKSGRPILLKRGMYSTLEEWLNCAEYILA
                     EGNPDVILCERGIRSFETYT"
     gene            complement(954..1277)
                     /gene="trxA"
                     /locus_tag="PLQ29_08850"
     CDS             complement(954..1277)
                     /gene="trxA"
                     /locus_tag="PLQ29_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPG86792.1"
                     /translation="MSAGIELTSKNFTAEVEQSTIPVLVDFWAEWCMPCRMIGPHIDD
                     LAKTYAGRLKVGKVNVDNEAELAGKFNIISIPTLMVFKDGKVVRQQPGALPKQAIEKL
                     FADQL"
     gene            complement(1426..2394)
                     /locus_tag="PLQ29_08855"
     CDS             complement(1426..2394)
                     /locus_tag="PLQ29_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86793.1"
                     /translation="MSRALPSRHFGVAEAASEDALAALRAEVPDAYTIVRSRFEAVDG
                     MRAALERLPPTAAASRLGSEPRTEDSLIAELLRGDRGDRLLYLPSRAVFRRSVVAAEA
                     QALAALARACAGAGIRGERLAAAQELATRAVLALMAEDAYLDILDGSAPEDARRSAAS
                     RLISLWDSDLDGGLDDSTEALVAVWTSRKSCRPVFGTLLGSSELVGLSMSLDGRWFAF
                     AGDRFSDPGVYQALAEFLFGLSHEELVACTAHIDANGPQDAAGIDALFGGVREYPEPA
                     SADPRDLYRFYEARRQAAKRREASGAAGPRSTLEGEYLLWAIGRRA"
     gene            complement(2391..2786)
                     /locus_tag="PLQ29_08860"
     CDS             complement(2391..2786)
                     /locus_tag="PLQ29_08860"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HPG86794.1"
                     /translation="MNDTCILLGLPDYRKLSGVVAAMKANRRLRDLHLKRLENELSTA
                     DVVEPDDLPGDVVSIGSSATVIDVDTGERFTTRLVFPAELDGTSATASVLSPLGVAII
                     GERVGANVSCVSPSGTKRFVVEAVARAER"
BASE COUNT          487 a          950 c         1036 g          495 t
ORIGIN      
        1 cgtataggtt tcgaagctgc ggatgccccg ttcgcagagg atgacgtccg ggttgccctc
       61 ggctaggatg tactcggcgc agttaagcca ttcctcgagg gtcgagtaca tgccgcgctt
      121 gaggagtatc ggccggccgg acttgcccgc ctcgcgcagc agcgagaaat tctgcatgtt
      181 cctggcgccg atctggagca cgtcggcgta ttcgccgacc cagctgacgt cgcgggtgtc
      241 tatcacctcg gtgacgatgg gcaggcccgt ctcgcgcctc gccttgtcca ggatcttgag
      301 ccccttgagg ccgagtccct ggaacgcgta cggcgaggtg cgcggcttga aggcgccgcc
      361 gcggagcatc gtcgctcccg cttccttgat cgctacggcc gtgcgcagga gctggtcctc
      421 ggactccacg gcgcatggtc ccgcgatgac ggtgaagccg tcgcctatgc gtatgccgcc
      481 gacctcgacc acggtcctcg ccccgtcggg ccggactccg gtcagcttca gatccttcat
      541 gatgtctacc ttccgccggc tgggccggcc gtgatcgtac ctaatatagt cgtcgttcgg
      601 tttgccctgg cccccgatcg cgacggcgcc cgcgacggaa ggatacaacc gggggcgccc
      661 cgtcgtcgat agcggcgcgg ctatcggagc atcgctcgcg aagcggagct cgcggggcgg
      721 cggccgggaa ccctacgggc tccccgcccg gccgcgccgc gatggagccc tatgcggtat
      781 aacggctata aaaatactgg tgggaggggg aaggggagta ggttggaact gatcgtcctg
      841 aaaccatgag ggcacgctac catgggccgg aaaaagaatc aagttactgt cggaagaaac
      901 tctctccttc ggcgaaggcg cggcgcgtcc gcgggccgca gcccgacgga cgatcagagc
      961 tgatcggcga acagcttctc gatggcctgc ttgggcaggg cgccaggctg ctggcgcacg
     1021 accttgccgt ccttgaagac catcaaggta gggatcgaaa tgatgttgaa cttgccggcg
     1081 agctcggcct cgttgtcgac gttgaccttg ccgaccttga gccgaccggc gtaggtcttg
     1141 gccaggtcgt cgatgtgcgg gcctatcatc cggcacggca tgcaccactc ggcccagaag
     1201 tcgacgagta ccggtatcgt cgactgctcg acctcggccg tgaaattctt ggaggtcaac
     1261 tctattcctg cgctcatagg ggctccttcg gtcgctcgaa cgcatcgggc gtcgcgtagg
     1321 cgcgcgcgat agttcgtagg ttatagcgat caaaataccg agcgggcggg cagattgtca
     1381 aatagagcag gggaaggcgc ggcccaaggc tagcccggcc tgtccttacg cgcgcctacc
     1441 tatcgcccag agcagatact cgccctcgag tgtcgacctg ggacccgcgg ccccggaggc
     1501 ctcgcggcgt ttggccgcct ggcgcctggc ctcgtagaag cggtataggt cgcgcgggtc
     1561 ggcgctcgcc ggttcggggt actcgcggac cccgccgaac agggcgtcga tccccgccgc
     1621 gtcctggggg ccgttcgcgt cgatatgcgc cgtgcaggcc acgagttcct cgtggctcag
     1681 gccgaacagg aattcagcta gcgcctgata gacgcccggg tcggagaagc gatcgccggc
     1741 gaaggcgaac cagcgcccgt cgaggctcat ggagaggccg acgagctcgc tcgagccgag
     1801 gagcgtgccg aacaccggcc gacatgactt gcgggaggtc cagacggcca cgagggcctc
     1861 cgtcgagtcg tcgaggccgc cgtccaggtc ggagtcccag agcgatatga gccgagaggc
     1921 ggccgagcgc ctggcgtcct ccggcgccga gccgtcgagg atgtccaggt aggcgtcctc
     1981 ggccatcagc gccagcacgg ccctcgtggc tagctcctga gctgcggcga ggcgttcccc
     2041 gcggatgccg gccccggcgc aggcccgggc cagggcggcc agcgcctggg cttcggccgc
     2101 gacgaccgag cgcctgaaca cggcccgaga gggcaggtag agcaggcggt cgcccctgtc
     2161 gccgcgtagt agctccgcga tcagcgaatc ctccgtcctc ggctccgagc cgagccggct
     2221 ggctgccgcc gtcgggggca agcgctcgag cgcggccctc atgccgtcga cggcctcgaa
     2281 gcgggaacgg actatggtgt aggcgtcggg cacctcggcg cgcagggcgg ccaaggcgtc
     2341 ctcgctcgcc gcctcggcga cgccgaagtg gcgcgagggg agcgctcggc tcatcgctcc
     2401 gctcgggcga ccgcctccac gacgaagcgt ttggtgccgg acggcgagac gcaggagacg
     2461 ttcgcgccga cccgctcgcc gatgatggcc acaccgagcg gggacaggac gctcgcggtg
     2521 gcgctcgtgc cgtcgagctc ggcggggaag acgagcctgg tggtgaagcg ctctcccgtg
     2581 tcgacgtcga tgaccgtggc cgaggagccg atcgagacca cgtcgccggg caggtcatcg
     2641 ggctcgacga cgtcggccgt cgacagctcg ttctcgaggc gcttcaggtg gaggtcgcgc
     2701 agtcgtcgat tggccttcat ggccgcgacg acgccggata gcttccgata gtccggaagc
     2761 ccgaggagga tgcatgtgtc gttcacggtg atgctccggg ggaggtgatg gaaacgatag
     2821 gtatcggttc aggtagagaa tacgactgaa tcagacgagg ggcaagattg gtataacgag
     2881 tatataactt gtatataatg ctacaagtct ctatcgttgc gcgctatcgc cgtcgagagg
     2941 ctaccctccc tggccggcat gccgagct
//
LOCUS       DAOPHV010000326         2967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_46461_length_2967_cov_4.467720, whole genome shotgun sequence.
ACCESSION   DAOPHV010000326 DAOPHV010000000
VERSION     DAOPHV010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46461_length_2967_cov_4.467720"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1569
                     /locus_tag="PLQ29_08865"
     CDS             <1..1569
                     /locus_tag="PLQ29_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86795.1"
                     /translation="AVGGRVEDGIALRGLSLIVSKLGSDDPELVVASSANGVFREAVD
                     LRALAPGWYNIQALAVDMAGNEAYDSRNIVVLESRKADYAELVFPAHGERIAGRFTID
                     GRVVSAEPIRRAAVTLDGQPFATVELGAGGWFSLAVEPGSVADGRRSFRIEAVSPSGA
                     TIASEPRELEYRLDGPWVDIGSLASGDFVIGRPYLTGSAGWDTPAPESPDKEAQAAYK
                     RLAASRKPTRVEISRDNGKTWQDASGAAAFKYRLETQEYPDGVLRLTVKATFAGGDVA
                     VRKRMVVLDTRPPAVELLKPAEGGRYNGVVSVEGVASDANGLSEVSVLLRSGDKSRYE
                     IPGFIQGSYADLHLLGATRFEAGLGLSFFEDNVRLQVELGRGFDAQPSWTNLLGFATD
                     DTPASELSRFGGYVLGARLLANVAYLPFSYWFGPDWDFFSMSFTLGASFTYFSMRDAL
                     GQIFSPPDDRYMVLSGVVGQWEFAKFTFDSRVLKSVGLYFEGGFVFIPSEASTRLEEF
                     IRPNIAFGARIGLF"
     gene            1723..>2967
                     /locus_tag="PLQ29_08870"
     CDS             1723..>2967
                     /locus_tag="PLQ29_08870"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HPG86796.1"
                     /translation="MQRRIKVLLAITSPDEASRIDAALTEAACLCGYAEDLRSVEQSM
                     GDSDVIVTDTEFASGAFADWLSLWPIPAVLVTDLDADPAKLAADLVDESSSFIRRDPA
                     GLWVSFMPMLVRKAAAIRESLDRQNSNIIRAESSYMSLLRAVPDIVYVLDGDGCFAYL
                     NDAVSQLGWKPSELIGKHFAEIVHPDDLPEVCRSLVLGRYEGVMTGAEAAPKLFDERR
                     SGDRMTRNLEVRLRRRNSGAWADTSVDSWGEVTSLGVRLPEFQGRGTGTIGLIHDVSA
                     RRENERRMSKELDARELLLKEIHHRVKNNLQVVSSLLSLESDCVEDEKAKAVFVDCQT
                     QVQSMSLVHEQIYRGSSLDGVEASAYFARLAEYLAMVHDARNRGIRLLVEPTDVVLSI
                     DTAMPLSIIATELVSNSFKHGYP"
BASE COUNT          506 a         1034 c          936 g          491 t
ORIGIN      
        1 gccgtgggcg gccgcgtcga ggacggcata gccctgcgcg gcctcagcct gatcgtctcc
       61 aagctaggct cggacgatcc cgagctggtc gtcgcatcgt cggccaacgg cgtattcagg
      121 gaggccgtcg acctccgcgc cttggccccg ggctggtaca acatccaggc gctggccgtc
      181 gacatggccg gcaacgaggc ctacgactcg aggaacatcg tcgtcctcga gtccaggaag
      241 gccgactacg ccgaactcgt gttcccggcc cacggagagc ggatcgccgg gcgcttcacg
      301 atagacggcc gggtcgtctc ggccgagccc atccgccgcg cggccgttac cctcgacggc
      361 cagcccttcg cgaccgtcga gctcggcgcc ggcggctggt tcagcctggc cgtggagccg
      421 gggtccgtgg ccgacggccg ccgatccttc aggatcgagg ccgtatcgcc ctccggcgcg
      481 accatcgcct ccgagccgcg cgagctcgag taccgcctgg acggcccctg ggtggacata
      541 ggctccctgg cctccggcga cttcgtcatc ggacgcccct acctgaccgg ctcggccggc
      601 tgggatacgc cggcgcccga atcgcccgac aaggaggccc aggcggcgta caagaggctc
      661 gcggctagcc gcaagccgac gagggtggag atcagccgcg acaacggcaa aacctggcag
      721 gacgcctccg gcgcggccgc cttcaagtac cgactcgaaa cccaggaata cccggatggc
      781 gtcctcaggc tgacggtcaa ggccaccttc gcgggcggcg acgtcgccgt gcgcaagcgc
      841 atggtcgtgc tggacacgag gccaccggcc gtcgagctcc tcaagccggc cgagggcggc
      901 cgctacaacg gcgtcgtatc ggtcgagggc gtcgcgtcgg acgcgaacgg cctgtccgag
      961 gtatcggtgc tcctgcgttc cggcgacaag tcgaggtacg agataccggg cttcatccag
     1021 ggctcctacg ccgacctgca cctgctcggc gccacccgct tcgaggctgg cctcggcctg
     1081 tcgttcttcg aggacaacgt gcgcctccag gtggagctcg gccggggctt cgacgctcag
     1141 ccgtcttgga ccaacctgct cggcttcgcg acggacgata cgcccgcctc tgaactatcg
     1201 cgcttcggag gctatgtgct cggcgccagg ctcctggcca acgtggccta cctgccgttc
     1261 tcgtactggt tcggcccgga ctgggacttc ttctcgatgt cgttcacgct cggcgccagc
     1321 tttacctact tcagcatgcg cgacgccctg ggccagatct tctcgccgcc cgacgatcgc
     1381 tacatggtgc tgtccggcgt ggtcggtcag tgggaattcg ccaaattcac cttcgacagc
     1441 cgcgtgctca aatcggtcgg gctgtacttc gagggcggct tcgtgttcat accgtcggag
     1501 gcttcgacga ggctggaaga attcatccgc ccgaacatag ccttcggcgc ccgtatcggc
     1561 ctcttctgac ccggcgatcc gcgggggggc gccagccctc ccgcggatca ccgcgccgcc
     1621 ccgccggcgc gggcttgaca gattccaata gtcgaactag ccttaaatct gttaaatgtc
     1681 aaatacaatt aaaattacgc acgacgaagg agtcctgagc ctatgcaaag aaggatcaag
     1741 gtccttttag cgatcacctc ccccgacgaa gcctcgcgca tcgacgccgc cctgaccgag
     1801 gccgcgtgtc tctgcggcta cgccgaggac ctgcgctcgg tcgagcagag catgggcgac
     1861 tccgacgtga tcgtgactga cactgaattc gcctccgggg ccttcgccga ttggctgtcg
     1921 ctctggccaa tacccgccgt actcgtcacc gacctggatg ccgatccggc caagctagcc
     1981 gccgacctcg tcgacgagtc cagctccttc atccgacgcg atcccgccgg gctctgggtc
     2041 agcttcatgc cgatgctcgt acgcaaggcc gcggccatcc gcgagagcct cgaccgccag
     2101 aacagcaaca tcatcagggc cgagagcagc tacatgagcc tcctccgggc cgtgccggac
     2161 atcgtctacg tgctcgatgg cgacggctgc ttcgcctacc tgaacgacgc cgtgtcgcag
     2221 cttggctgga agccgtccga gctgataggc aagcatttcg ccgagatcgt ccaccccgac
     2281 gacctgccgg aggtctgccg cagtctggtg ctcggccgct acgagggcgt catgaccgga
     2341 gccgaggccg cgcccaagct attcgacgaa cgccgctccg gcgaccgcat gacccgcaac
     2401 ctcgaggtca ggcttcggcg cagaaactcg ggcgcatggg ctgacacttc cgtcgactcg
     2461 tggggcgagg tgacgagcct aggcgtacgg ctcccggaat tccagggcag gggcaccggc
     2521 acgataggcc tgatccacga tgtcagcgcc cgccgcgaga acgagcgccg gatgagcaag
     2581 gagctcgacg cgagagagct cctcctcaag gagatccacc accgggtcaa gaacaacctg
     2641 caggtggtct cgagcctact gtctctcgag agcgactgcg tcgaggacga gaaggccaag
     2701 gccgtcttcg tcgactgcca gacgcaggtg cagtcgatgt cgctggtcca cgagcagatc
     2761 taccgcggct cgtcgctcga cggcgtcgag gccagcgcct atttcgcgag actcgccgag
     2821 tacctggcca tggtacacga cgccaggaat cgcggcatac gcctattggt ggagccgacc
     2881 gatgtcgtgc tgtccatcga caccgcgatg ccgctatcga tcatagcgac cgagctcgta
     2941 tcgaactcgt tcaagcacgg ctacccc
//
LOCUS       DAOPHV010000327         2960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_46613_length_2960_cov_3.760069, whole genome shotgun sequence.
ACCESSION   DAOPHV010000327 DAOPHV010000000
VERSION     DAOPHV010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46613_length_2960_cov_3.760069"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PLQ29_08875"
     CDS             <1..888
                     /locus_tag="PLQ29_08875"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HPG86797.1"
                     /translation="FLVPIQYTGARRGFKAMVGSAAVSSIVIAAWQTIALARADILSA
                     GTVAVGVSTPLALAIALVAMAWPRLARFSFTIRALVAAAVASALSLPALFAAIRDPAV
                     RAMFLDAFETASSALGAEVMDAQALWSALRTGVASSYGAVLFAFLFLSSWSGSSLGRA
                     MDARTGGRDDAPESLTIQVPNLAAYRVPRGLVWALLAAWTALLVNRFVSVPVFSAVAL
                     NAALALSICYGVQGLAVVRALAERVGLAPALRLIGPLAIILLLVSGVGGFVAIGLLAL
                     LDTLETWIPFRAVTEGESP"
     gene            885..1454
                     /gene="rplI"
                     /locus_tag="PLQ29_08880"
     CDS             885..1454
                     /gene="rplI"
                     /locus_tag="PLQ29_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPG86798.1"
                     /translation="MKVILNQDVPNLGELGDVKDVAAGYARNFLLPRGLVYPFSPATV
                     ALFEKRRAEIEAHKAAKRASSSDLKTKLEAEELTLDMPAGQNGKLFGAVSNSTVADEL
                     LKKGLQVDRKKIEVPGRVIKSVGNYKIVVRLYEKDEAIVRVVVKGHVEKAKQAAEQPR
                     RHREEPVKAVETEPAEVETPAETVEGDQA"
     gene            1464..2809
                     /gene="dnaB"
                     /locus_tag="PLQ29_08885"
                     /pseudo
     CDS             1464..2809
                     /gene="dnaB"
                     /locus_tag="PLQ29_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624494.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
BASE COUNT          556 a          979 c          913 g          512 t
ORIGIN      
        1 ttcctggttc ccattcaata cacgggagcc aggcgtggct tcaaagccat ggtagggagc
       61 gccgcggtct cgtcgatcgt aatcgccgcc tggcagacta ttgccttggc cagggccgat
      121 atcctgagcg ccgggaccgt agctgtcggc gtctcgacgc ccctggccct ggcgatcgcc
      181 ctcgtcgcga tggcatggcc caggctcgcg cgtttctcgt tcacgatccg cgccctcgtg
      241 gccgcggcag tcgcctcggc actgagcctg ccggccctgt tcgccgcgat acgggatccg
      301 gccgtgcgag ccatgttcct cgacgcgttc gagacggcta gctcggccct cggcgcggag
      361 gtcatggacg cccaggcgct atggtcggcc ctccggacgg gagtggcgtc cagttacgga
      421 gccgtgctgt tcgcgttcct attcctgagc tcgtggtctg gctcgagcct cggccgcgcc
      481 atggacgcgc ggaccggagg ccgtgacgat gcgcccgagt cgctgacgat tcaggtgccg
      541 aacctggccg cctatcgcgt tcctcgcggt ctcgtatggg cgctgctagc ggcctggacg
      601 gcgttgctcg tcaaccgttt tgtttcagtt cccgtgtttt cggcggtcgc gttgaacgcg
      661 gccttagcat tatcgatctg ctacggcgtc cagggtctcg ccgtcgtccg cgcgctagcc
      721 gaacgcgtcg ggctggcccc tgccttgcgg ctcatcggcc ccttggcgat tatcctcctc
      781 ctcgtaagcg gggttggcgg gttcgtcgcc atcgggctcc tggccttgct cgacacccta
      841 gagacctgga tcccgttccg tgcagtaacc gaaggagaat caccatgaag gtaatcctga
      901 accaggatgt cccgaacctg ggcgaactcg gagatgtgaa ggacgtcgcc gccggatacg
      961 cccgcaactt cctcctgccc cgcgggctcg tctatccgtt cagccccgcg acggtagcgc
     1021 tgttcgagaa gcgtcgcgcc gagatcgagg cccacaaggc cgcgaagcgc gcctccagct
     1081 ccgacctcaa gaccaagctc gaggccgagg agctgacgct cgatatgccg gccggtcaga
     1141 acggtaagct tttcggagcc gtatcaaact cgaccgtcgc cgacgagcta ctcaagaagg
     1201 gcctccaggt cgaccgcaag aagatcgagg tccccggacg cgtcatcaag agcgtcggca
     1261 actacaagat cgtcgtccgc ctctacgaga aggatgaggc catcgtccgc gtcgtcgtca
     1321 agggccacgt cgagaaggcc aagcaggccg ccgaacagcc caggaggcac cgtgaggagc
     1381 cggtcaaggc cgtcgaaacc gagcccgccg aggtcgagac gccggccgag accgtcgaag
     1441 gcgatcaggc ataaacccga cgcatgatcc ccgcccagct caaggacaag gtcccgcctc
     1501 ataacgccga ggcggaacag gccgcgctgg gcgccctgct tctcgatccg gacgcggtgc
     1561 cgcatatact gcggtatctg cgtccggacg acttctatgt aaacgccaat aagaacgtct
     1621 tctcggccat tgtctccctg ttcgagaagg ggcagaaggc cgacctcatc acgctcgccg
     1681 acgagatgcg ggtgctcggc tccctcgaca actcgggcgg gccggcctat atcgccaagc
     1741 tgaccgactc ggtgccgtcc agcgccaata tggagtacta cgccaagatc gtgcaggaat
     1801 gctcgacgag gcgcagcctg ctccgactag ccgccgatat cagccagaag gcccacgacg
     1861 agtccatcga caccggcctc ctgctcgacg agctgcagga aagcatcttc gaggtaaacc
     1921 aggaccgcca gaccgtctcc tatcgcagcg tacgcgagat cgtgcccgac gcgatgaaga
     1981 tgatcgagga actggccaag aacaagaacc agtacaccgg cgtaccgagc ggcttcgccg
     2041 agctcgatag catgaccagc ggtttccaag actcggagct gatcatcatc ggcgcccgcc
     2101 cgagcgtcgg taagacggcc ttcgcgctca atatcgcggc tcatgtagcg ctgcgcgagc
     2161 gcctgccggt ctgcttcttc tcgctcgaga tgtcggacct gtcgctgacc taccgtatcc
     2221 tgtcgagcga ggccaggata gactcggaaa aggttaagag cggcctgatc aagccgagcg
     2281 acatccagag cctgatggac gccgcgagcc ggatctacga ctcgccgatg tacatcgtcg
     2341 acgtgccgaa catcaagttg ctcgatctgc ggtcgctggc caggcgcatg aagagcgaga
     2401 aggacgtcaa agtcatcttc atcgattacc tgaccctgat cacccatgag aacgccgact
     2461 tgccgcgctg ggagcagatc tcgagcatct cgcgttcgct caaggccctg gcccgcgagc
     2521 tacgcatccc cgtcatagcc ctgtcgcagc tgaagcgcga ggccgagggt aagcagccgt
     2581 cgctcgccga cctccgcgag tccggctcca tcgagcagga cgccgacctg atcatgttct
     2641 gcaccgcgat cgcgatatcg acaagcgcaa ggacgagcgg gcaccgtata cgccgacgga
     2701 cctgctcgtc gcgaagcacc gcaacggacc ggttggcaag gtcgagctgc tgttcttcaa
     2761 caagatcacc aggttcgagt cgcttgaaaa ggaacgcgcc gagcattaga ctcggcctac
     2821 ggaggatccg catggatatc gcaaaaggct acgacctgag cttcctcgtc aacgacgcgg
     2881 agcgcatggt gctcgacgag ctgggacgcg tgctcgatac cgccgagggc gacggcatct
     2941 gcgtctgcca ggactgcgtc
//
LOCUS       DAOPHV010000328         2953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_46760_length_2953_cov_5.117322, whole genome shotgun sequence.
ACCESSION   DAOPHV010000328 DAOPHV010000000
VERSION     DAOPHV010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46760_length_2953_cov_5.117322"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..490)
                     /locus_tag="PLQ29_08890"
     CDS             complement(119..490)
                     /locus_tag="PLQ29_08890"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HPG86799.1"
                     /translation="MSERKELTCIECPRGCRLTVEVDGARAMVSGNACPKGAVWGAQE
                     AVSPMRVLTTTVAVDGSARKRLPVRSDGVLPLGRLLDAMTAIDPIVVRPPIRRGDVVV
                     RDLLGLGVDLVATDDLLGDGR"
     gene            complement(487..1836)
                     /locus_tag="PLQ29_08895"
     CDS             complement(487..1836)
                     /locus_tag="PLQ29_08895"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG86800.1"
                     /translation="MIRRFETDVVVLGAGPAGLAAACAAMEAGARVVVVEREERTGGI
                     LKQCIHDGFGLARYGRQLSGPEYALRDADRFGALGGLAMLEAFVTSAVRKDGGGFELA
                     CVDRGGACLLRSAALVLATGCRERTDRQVFVHGDRPAGVFTAGLAQYMINVQGLLPGR
                     RAVILGSGDIGLIMARRMTLEGMEVLGAYEAKPEPSGLSRNLRQCLDDYGIPLTLSST
                     VTAVHGERRVEGVTTALVDGSMRPIPGTERDLSCDTLVLSVGLIPENELAKSLGVPLD
                     PRTRGPRVDQTMHTLVDGVFACGNAVHVNDLADYVSESGERAGSAAAAYARSGPGSAG
                     RKRRLSPLVAGAGCLYVVPQLLDLDALDRAAGTRDDLCVYFRSNRTMPVGATARLAVG
                     DGPGGAALLERRYRALRPPEMERIKAATGALAAPLASQAEPGRGASLTIEPDGVEDR"
     gene            complement(1833..>2953)
                     /locus_tag="PLQ29_08900"
     CDS             complement(1833..>2953)
                     /locus_tag="PLQ29_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG86801.1"
                     /translation="ALFLPSAGILSPYRATVALAESAAMNGARVALGACVLGMGLEGG
                     SISSLTTDRGTFRAGIVVNAAGNWADVVAGMAGDRFFSLHQRRGTDMILDAETASAQR
                     HILGMPRLLQLHSKTKGGGLVLTVEGNVLVGPTAAEAPGREDYATRPEELDQLTRHLR
                     LNTALSMAQGITYFSGVRPCTYEEDFIVEKSERVTNLVHAAGIQSPGLASAPAIAQDV
                     AAMVVELAGRFKRVSPRAAWRAERPAPREPRRLRLEARDALIAGNPAYGRVVCRCEEI
                     SEGEIVDAIRSIVPATDLDAIKRRTRAGMGRCHGGFCTPRVMELVARETGLPAWAVTK
                     KGPGSELCVGPTKASPPRFSADAAHADAAHADAGGIEP"
BASE COUNT          419 a         1047 c         1094 g          393 t
ORIGIN      
        1 cccagagcct tcgggcgctg gccttcgacg ggcgtggcgc gctcgtcgaa ggccagcgcc
       61 cgaaggctct gggtgccgca gtcaatggcc agaaccaggt cggccgagga cgcggcgctc
      121 atcgtccgtc cccgaggagg tcgtcggtgg cgacgaggtc gacgccgagg ccgaggaggt
      181 cgcgaacgac gacgtcgccg cgacggatcg gcggacggac gacgatgggg tcgatggcgg
      241 tcatcgcgtc gaggaggcgg ccgagaggaa ggaccccatc ggagcgcacc ggaaggcgct
      301 tcctggccga gccgtcgacc gcgacggtcg tggtcaggac gcgcataggg gacacggcct
      361 cctgagcgcc ccaaacggca cccttggggc aggcgttgcc gctcaccatc gcgcgagcgc
      421 cgtccacctc gaccgtcagc cggcagccgc gagggcattc gatacaggtc agttccttgc
      481 gctcgctcat cggtcctcca ctccatctgg ttcgatcgtt aggctggcgc cgcgccccgg
      541 ctccgcctga ctagccagag gggcggccag ggcgccagtg gccgccttga tacgctccat
      601 ctcgggcggg cgcaaggcgc ggtaccgccg ctcgagtagc gcagccccgc cgggcccgtc
      661 cccgacggcc agtctcgcgg tcgcgccgac tggcatggtc cggttcgagc ggaagtagac
      721 gcataggtcg tcgcgagttc cggcggcgcg atcgagcgcg tccaggtcca ggagctgcgg
      781 cacgacgtag aggcagccgg cccccgcgac gagcggcgac aggcggcgct tacggcccgc
      841 cgagccgggg ccggagcgcg cgtacgccgc cgcggccgag ccggcccgct cgcccgactc
      901 ggagacgtag tcggccaggt cgttcacatg gaccgcgtta ccgcaggcga acaccccgtc
      961 gacgagggtg tgcatggtct ggtcgacccg cgggccgcgc gtccgcgggt cgagcggcac
     1021 accgaggctc ttggccagct cgttctcggg tatcagtccg accgacagca cgagggtgtc
     1081 gcagctcagg tcgcgctccg tgccgggtat cggccgcatc gagccgtcga cgagggccgt
     1141 cgttacgccc tcgacgcggc gctcgccgtg aaccgcggtg accgtggacg acagggtcag
     1201 cgggatgccg tagtcatcga ggcactggcg caggttgcgc gacaagcccg agggctccgg
     1261 tttggcctcg taggcgccaa gcacctccat gccctcgagg gtcatgcggc gggccatgat
     1321 caggccgatg tctccggagc cgaggatcac ggcgcggcgg ccgggcagga ggccctgcac
     1381 gttgatcatg tactgggcca ggcccgcggt gaacacgccg gccggccggt cgccgtgcac
     1441 gaagacctgg cggtcggtgc gctcgcggca accggtcgcc agtacgaggg ccgcggaccg
     1501 tagcaggcag gcgccgccgc ggtcgacgca ggccagctcg aagccgcctc cgtccttgcg
     1561 aaccgccgac gtgacgaagg cctcgagcat ggccaggccg ccgagcgcgc cgaagcggtc
     1621 ggcgtcgcgc agggcgtact cgggcccgga cagctggcgg ccgtagcgcg ccaggccgaa
     1681 accgtcgtgg atgcactgct tgaggatgcc gccggtccgc tcctcgcgct ctacgacgac
     1741 gacgcgggcg ccggcctcca tcgcggcgca ggccgcggcc aggccggctg ggccggcgcc
     1801 gagcaccacg acgtcggtct cgaaacgccg gatcacggct ctatcccgcc ggcgtcggcg
     1861 tgcgcggcat cggcgtgcgc ggcatcggca gagaagcgcg gcgggctggc cttggtcggg
     1921 ccgacgcaca gctccgagcc cgggcccttc ttggtaacgg cccaggccgg cagccccgtc
     1981 tctcgcgcca cgagctccat gacccgcggc gtgcagaagc cgccgtggca gcggcccatg
     2041 cccgcccgcg tgcggcgttt gatcgcatcc aggtcggtgg ccggcacgat cgagcgtatc
     2101 gcgtcgacga tctcgccctc ggagatctcc tcgcagcggc agacgacgcg gccgtaagcc
     2161 gggttcccgg ctatcagcgc gtcccgggcc tcgaggcgga gtcgcctcgg ctcgcgcggc
     2221 gccggccgct cggcccgcca ggcggctctc ggcgacacgc gcttgaagcg gccggccagc
     2281 tcgacgacca tagccgcgac gtcctgggct atagccggcg ccgaggccag gccgggcgac
     2341 tggatgccgg ccgcgtgcac aaggttcgta acgcgctcgc ttttctcgac gatgaagtcc
     2401 tcctcgtagg tgcagggacg cacgccggag aagtaggtga tgccctgggc catcgacagc
     2461 gccgtattga gccgcaggtg cctggtcagc tggtcgagct cctccggcct cgtcgcgtag
     2521 tcctcgcggc ccggggcctc cgcagcggtc ggcccgacga gcacgttgcc ctcgacggtc
     2581 aggacgaggc cgccgccctt ggtcttcgaa tggagctgga ggaggcgggg catgccgagg
     2641 atatggcgct gggccgaggc ggtctcggcg tccagtatca tgtcggtgcc gcgccgctgg
     2701 tgcaggctga agaatcggtc gccggccatg ccggccacga cgtcggccca gttgcccgcg
     2761 gcgttgacca cgataccggc tcggaacgtg ccgcgatcgg tggtcagcga cgatatcgag
     2821 ccgccctcga gccccatgcc gagtacgcag gcgccgagcg ccacgcgcgc gccgttcatc
     2881 gcggcgctct ccgctagggc gaccgtggcg cggtacggcg acaggatgcc ggcgctcggt
     2941 aagaacaagg cgc
//
LOCUS       DAOPHV010000329         2948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_46902_length_2948_cov_4.284134, whole genome shotgun sequence.
ACCESSION   DAOPHV010000329 DAOPHV010000000
VERSION     DAOPHV010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46902_length_2948_cov_4.284134"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="PLQ29_08905"
     CDS             <1..1121
                     /locus_tag="PLQ29_08905"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPG86802.1"
                     /translation="VGHKQFTEIAREINRENVRLLVLDEPTAVLTESEAEILLEALRR
                     LAAQGISIIFISHRLHEITKVADTIVVLRDGKVVKEAPSEGTSVRDIAAWMVGRSVEA
                     KRPDLATRSFGEEALRVERLWVDMPGESVRDVSFSVRSGEIFGIGGLAGQGKLGVPNG
                     IMGLYAGGGSVTLHGKSVPLNDPKAALAMGMAFVSEDRRGVGLLLDESLEWNIGFTAM
                     QIRGDYLKRYLGGLLKLRDEAGLRKLAEEYIELLSIKCTSPKQFAKELSGGNQQKICL
                     AKAFAMQPDLLFVSEPTRGIDVGAKELVLKSLRHYNRDNGTTIVMVSSELEELRSICD
                     RVAIVDEGRIAGILPPDADIADFGLLMAGEVKEEAAHA"
     gene            1114..2157
                     /locus_tag="PLQ29_08910"
     CDS             1114..2157
                     /locus_tag="PLQ29_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG86803.1"
                     /translation="MRKIKEFAADFGWPRIIIFLFLVSLFIVAPFVGVSISRSLSDTL
                     NRFGQNAILVLALVPMVQSGCGLNFGLPVGIIAGLLGATVAMQLGLTGWLGFLGAVAI
                     CIPFGLLLGWLYGQLLNKVKGEEMTIAMYVGFSIITFFSILWLLLPYSNPAMIWGYGG
                     AGLRTTITLDNYWSKILNDFLAVRIGGSFVFPTGMILFFALCAFFVYLFFRTKTGTAM
                     TAVGSNPDFARAGGVSADKMRTVSVMLSTVLGAVGILVYQQSFGFIQLYQAPLYMAFP
                     AVAALLIGGASVNKASMVNVVVGTLLFQGILTMTPSVINSILQTDMSEVIRIVLSNGM
                     ILYALTRKTKVSK"
     gene            2154..>2948
                     /locus_tag="PLQ29_08915"
     CDS             2154..>2948
                     /locus_tag="PLQ29_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG86804.1"
                     /translation="MTKRFDFKRFVADNVVVVIFLVLTAIATPISGLSANYIIQDIIT
                     RIGRNSFLVFSLLLPVMAGMGLNFGMVLGAMAGEIGLIFAVDWGISGVWGLGFASLVG
                     LPIAVALGWLCGSILNRAKGREMATGYIIGFFMDGVYQFAVLYLMGSLIPMRSLAITL
                     SRGYGIRNTLNLDSVRQSLDNLAMVKVFGFNVPVATYLVVAALCVFIVWFRKTKLGQD
                     MRAIGQDQGVSNASGIPVEKTRIIAIVVSTVLAMVGQIIFLQNMGNM"
BASE COUNT          490 a          957 c          928 g          573 t
ORIGIN      
        1 cggtaggcca caagcagttc accgagatcg cgcgcgagat caaccgcgaa aacgtcaggc
       61 tcctcgtgct cgacgagccg accgcggtgc tgaccgagag cgaggccgag atcctgctcg
      121 aggccctcag gcggctggcc gcccagggca tctcgatcat cttcatatcg caccgcctgc
      181 acgagatcac caaggtcgcc gataccatcg tcgtgctgcg cgacggcaag gtcgtcaagg
      241 aagcgccgtc cgagggtacc tcggtacgcg atatcgccgc ctggatggtc ggccgctcgg
      301 tcgaggcgaa gcgccccgac ctggcgaccc gctccttcgg cgaggaagcg cttcgcgtcg
      361 agcggctctg ggtcgatatg ccaggcgaga gtgtccgcga tgtgtcgttc tccgtacgct
      421 caggcgagat cttcggcatc ggcggcctgg ccggccaggg caagctcggg gtgccgaacg
      481 gcatcatggg cttgtacgcc ggcgggggct cggtaacgct gcacggtaag tccgtgccgc
      541 tcaacgaccc taaggccgcg ctcgcgatgg gtatggcttt cgtatccgag gaccggcgcg
      601 gcgttggcct gctcctcgac gagagcctcg agtggaatat cggcttcacc gctatgcaga
      661 tacgcggcga ctatctcaag cgctacctcg gcgggctcct caagctgcgc gacgaggcgg
      721 gcctccgcaa gctggccgag gagtacatcg agctgctgtc gatcaagtgt acgagcccca
      781 agcagttcgc caaggagctg tccggcggca atcagcagaa gatctgcctg gccaaggcct
      841 tcgcgatgca gccggacctg ctgttcgtca gcgagccgac ccgcggtatc gacgtgggcg
      901 ccaaggagct ggtgctcaaa tcgctacgcc actacaaccg ggacaacggc acgaccatcg
      961 tgatggtctc gagcgagctc gaggagctcc ggtccatctg cgaccgcgtc gcgatcgtcg
     1021 acgagggccg catcgccggt atcctaccgc cagacgcgga tatagctgac ttcggcctct
     1081 taatggccgg agaggtcaaa gaggaggccg cgcatgcgta agataaagga attcgcggct
     1141 gacttcggct ggccgcgcat catcatcttc ctgttcctcg tgtcattgtt catcgttgcg
     1201 cccttcgtcg gcgtatcgat cagccgctcg ctgtccgaca cgctcaaccg tttcgggcag
     1261 aacgcgatcc tggtgctcgc cctcgtcccc atggtccagt cgggttgcgg cctgaacttc
     1321 ggcctgccgg tcggcatcat cgccggcctg ctcggcgcga cggtggcgat gcagctcggc
     1381 ctgaccggct ggctcggctt cctgggcgcc gtggcgatct gcataccctt cggcctgttg
     1441 ctcggctggc tctacggcca gctcctcaac aaggtcaagg gcgaggagat gacgatagcc
     1501 atgtacgtcg gcttctcgat catcaccttc ttctcgatcc tgtggctgct cctgccgtac
     1561 tcgaaccccg cgatgatctg gggctatggc ggcgcgggtc tccggacgac gatcacgctc
     1621 gacaactact ggagcaagat cctcaatgac ttcctcgcgg tcaggatagg cggcagcttc
     1681 gtgttcccga ccggcatgat cctgttcttc gcgctgtgcg ccttcttcgt ctacctgttc
     1741 ttcaggacca agaccggcac cgcgatgacc gccgtgggct cgaacccgga cttcgcgagg
     1801 gccggcggcg tgtcggccga caagatgcgc accgtcagcg tgatgctgtc gacggtgctc
     1861 ggcgcggtcg gtatcctcgt gtaccagcag agcttcggct tcatccagct gtaccaggcg
     1921 ccgctgtaca tggccttccc ggccgtggcc gccctcctga taggcggcgc cagcgtcaat
     1981 aaggcctcga tggtcaacgt ggtagtcggc accctgctgt tccagggcat cctgacgatg
     2041 acgccgagcg tcatcaacag catcctgcag acggacatgt cggaggtgat acgcatcgtg
     2101 ctgtcgaacg gcatgatcct gtacgcgctg accagaaaga cgaaggtgtc gaaatgacga
     2161 agcggttcga tttcaagagg ttcgtcgccg acaacgtggt cgtcgtcatc ttcctcgtgc
     2221 ttacggccat cgcgaccccg atctcggggc tgtcggcgaa ctatatcatc caggacatca
     2281 tcacccgcat cgggcgcaac tcgttcctgg tgttcagcct gctcctgccg gtcatggcgg
     2341 gcatgggcct gaacttcggc atggtgctcg gcgcgatggc cggagagatc ggcctgatct
     2401 tcgcggtcga ctggggcatt tccggcgtct ggggcctcgg cttcgcctcg ctcgtcggcc
     2461 tgccgatcgc cgtggccctc ggctggctat gcggctccat cctgaaccgg gccaagggcc
     2521 gcgagatggc taccggctac atcatcggct tcttcatgga cggcgtctac cagttcgcgg
     2581 tgctgtacct gatgggctcg ctgataccga tgcgctcgct cgccatcacg ctgtcgcgcg
     2641 gctacggcat ccgtaacacc ctgaacctgg actcggtcag gcagagcctg gacaacctgg
     2701 cgatggtcaa ggtcttcggc ttcaacgtgc cggtcgccac ctacctcgtg gtcgcggcgc
     2761 tctgcgtgtt catcgtctgg ttcaggaaga ccaagctcgg ccaggacatg cgcgccatcg
     2821 gccaggacca gggcgtttcc aacgcctcgg gcatcccggt ggagaagacg cgcatcattg
     2881 ccatcgttgt ctcgacggtc ctagcgatgg tcggccagat catctttctc cagaacatgg
     2941 gcaacatg
//
LOCUS       DAOPHV010000330         2932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_47274_length_2932_cov_3.599930, whole genome shotgun sequence.
ACCESSION   DAOPHV010000330 DAOPHV010000000
VERSION     DAOPHV010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47274_length_2932_cov_3.599930"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1114
                     /locus_tag="PLQ29_08920"
     CDS             167..1114
                     /locus_tag="PLQ29_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86805.1"
                     /translation="MFGKRKEPAHVAFDARVKSYLIYLAYVKNSISLSIGDKSDLFKT
                     AEDISRDIFVSIEKLKASKVNQFNAREVEAAMAGTIKRIDRLIAAELKKIDKLVVIDH
                     SRTHSIAEAVASMVQSGPNLVAMSRDHVDRFFSVSKFIDPPAKPVDVKKETAKAAKAY
                     DDELYKLNLKAFLDTLASGDGQAINPEAYFKAATVLNDTLISGVGAPVGVGAPVGAGA
                     PGTAHNASTPAPSFSAEPDGGFLVSYRDHPVEVDLAVRVGDSGDRFTVDLRGRLVDEG
                     SSVDFSIDKRYFGSSRDGVMNAFKLRILLEEWKRRSGGR"
     gene            1205..2155
                     /locus_tag="PLQ29_08925"
     CDS             1205..2155
                     /locus_tag="PLQ29_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HPG86806.1"
                     /translation="MLKKTRSDATLIRDLPDFTRFMPFLMPNKGGSVINYEQDLDVTE
                     TLDLIKQVNRELIKEREILTLFGVVINAAVRAVAMRPKLNRFISGYRFWQRNQILVNF
                     VAKKEISDEGQEVNVKIDLSPYETLATTARKVRSAVKRAVSDDGAENEAVVSTIMRLP
                     DPLVRLLVRGMDWLDQRNLMPRSMTDSDPMWSSVFLTNVGSFGLDAPYHHLFERGNCP
                     VFLAMGKVREERLLDEEFRPVTRKMLRLRYTFDDRVADGVYMGRALDLVQRFVEHADE
                     LLEPPILSRELLAELRLRDYPAEAVQARAAEVPAAEGVRA"
     gene            2152..2742
                     /locus_tag="PLQ29_08930"
     CDS             2152..2742
                     /locus_tag="PLQ29_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86807.1"
                     /translation="MNVKFVAAALALIFGAALVRYYSERRKKRQDEAATRAGTLEGGA
                     AAPEEGVEMERVFLDEVAAGRRALRIASVYNQYDIMFVKSLFQSEQIPYYFEFENMSK
                     LRTGIPVFSFNNALLNVLEDDYDDAYAVLAAYKEGPRDGGTGDEGPGGEDARGAERIR
                     NAAEIVLGGWKSPSPGERAIELYDRPKTPRDPRNQE"
BASE COUNT          565 a          955 c          941 g          471 t
ORIGIN      
        1 cccctgttcg ctctcctcat gccgctctcc tacgtggcgc tcggatggtt cctcatcgcc
       61 aacaggctcg ggcttcacga cgatcgcagg ctgcgctata tcgacgacta tcggcccgcg
      121 taaggtacgc cagaaaatcc cctcaagcca atacaaggag tgtcccatgt tcggcaagcg
      181 taaggagccg gcccacgtcg cgttcgacgc cagggtcaag tcgtacctga tctacctggc
      241 ctacgttaag aacagcatct cgctgtccat aggcgacaag agcgacttgt tcaagacggc
      301 ggaggatata tcgcgcgaca tcttcgtctc catcgagaag ctcaaggcct ccaaggtcaa
      361 ccagttcaac gcgcgcgagg tagaggctgc catggcgggg acgatcaagc gcatcgaccg
      421 gctcatcgcc gccgagctga agaagatcga caaactagtg gtcatcgacc attcccggac
      481 gcactcgata gccgaggccg tcgcctcgat ggtccagtcg ggtccgaacc tggtggcgat
      541 gtcgcgcgac cacgtcgacc gcttcttctc cgtctccaag tttatcgacc cgcccgccaa
      601 gccggtcgac gtcaagaagg agacggctaa ggcggccaag gcctacgacg acgaactata
      661 taagctcaac ctgaaggcct tcttggacac gctcgcgtcg ggcgacggcc aggcgatcaa
      721 tcctgaggcg tacttcaagg ctgcgaccgt cctgaacgac actttaatat caggcgtcgg
      781 cgctcccgtc ggcgtcggcg ctcccgtcgg cgccggggcg ccgggcacgg cccataacgc
      841 gagtacgccg gctccttcgt tctcggccga accggatggc ggcttcctgg tctcctaccg
      901 cgatcatccc gtcgaggtcg acctggcggt acgcgtcggc gattcaggcg accgcttcac
      961 ggtcgacctc cgcggccgcc tagtcgatga aggctcgagc gtcgatttct ccatcgataa
     1021 acgctacttc ggctcgagcc gcgacggcgt gatgaacgcg ttcaagctgc gcatcctgct
     1081 cgaggaatgg aagcggcgct cgggagggcg ctaagcctat acgcgtcggt tccgggcgcg
     1141 tgacgccctc ccccggccgt gctatgattc cggaaccgcg agacacgacg gccgagaggg
     1201 aacgatgctc aagaagacga gatcagacgc tacgctgatt agagacctgc ctgactttac
     1261 ccgcttcatg cccttcctga tgccgaacaa gggcgggtcg gtgatcaact acgagcagga
     1321 cctggacgtc accgagacgc tggatctcat caagcaggtc aaccgcgagc tgatcaagga
     1381 gcgcgagatc ctgacgctgt tcggcgtggt gatcaacgcg gcggtgcgcg ccgtggcgat
     1441 gcgtcccaag ctcaaccgtt tcatctccgg ctaccggttc tggcagcgga accagatcct
     1501 cgtcaacttc gtggccaaga aggagatcag cgacgagggg caggaggtga acgtcaagat
     1561 cgacctcagc ccctacgaga ccctcgcgac caccgcgcgc aaggtgcggt cggcggtcaa
     1621 gcgagcggta tcggacgacg gcgccgagaa cgaggccgtc gtctcgacca tcatgaggct
     1681 gccggacccg ctcgtacggc tgctcgtgcg cggcatggac tggctcgacc agcgcaacct
     1741 gatgccccgc tcgatgaccg actcggaccc gatgtggtcg agcgtcttcc tgaccaacgt
     1801 cggctccttc gggctggacg cgccctacca ccacctgttc gaacgcggca actgcccggt
     1861 attcctggcc atgggcaagg tgcgggagga gcgcctcctc gacgaggaat tccgcccggt
     1921 gacgcgcaag atgctcaggc tgcgctacac cttcgacgac cgcgtcgccg acggcgtcta
     1981 catgggccgc gcgctcgacc tggtccagcg tttcgtggag cacgccgacg agctgctgga
     2041 accgccgata ctcagccgcg aactgctggc cgagctgcgg ctcagggatt accctgccga
     2101 ggccgtacag gctcgggccg ccgaggttcc ggccgcagag ggggttcgag catgaacgtc
     2161 aaattcgtcg cggcggcgct ggcgctcatc ttcggggcgg cgctcgtcag gtactacagc
     2221 gagcgtcgga agaagcgcca ggacgaggcg gcgacgcggg cgggaacgct cgagggtggc
     2281 gccgcagccc ccgaggaggg cgtcgagatg gagcgcgtgt tcctcgacga ggtggccgcc
     2341 ggcaggcgcg ccctcaggat agccagcgtc tacaaccagt acgacatcat gttcgtcaag
     2401 tcgctgttcc agtcggagca gataccgtac tacttcgagt tcgagaacat gtccaagctg
     2461 cgtaccggca tacccgtgtt cagcttcaac aacgccctcc tcaacgtgct cgaggacgac
     2521 tacgacgacg cctacgcggt gctcgcggcc tacaaggaag gaccgcgcga cggcgggacg
     2581 ggcgacgaag gccccggtgg cgaggacgcg cgcggcgccg agcggatacg gaacgcggcc
     2641 gagatcgtcc tcggaggctg gaagagcccg tcgcccgggg aacgcgccat cgagctgtac
     2701 gaccggccga agacgccacg ggatccgagg aaccaggaat agttttagcc tcgcggatcg
     2761 cccaggcgtg gtataacggt cgtgggagga cgccatgccc gactatggaa cgcccgctgg
     2821 cctcgacgaa gccgagctac agaaacgcgt gcgccacgat taccgcctgt ccgaggccga
     2881 ggtggaggcg cggctgcgcg ccgaactgcc agacctaagc gcggacgagc tg
//
LOCUS       DAOPHV010000331         2926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_47416_length_2926_cov_3.424591, whole genome shotgun sequence.
ACCESSION   DAOPHV010000331 DAOPHV010000000
VERSION     DAOPHV010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47416_length_2926_cov_3.424591"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1756
                     /locus_tag="PLQ29_08935"
     CDS             167..1756
                     /locus_tag="PLQ29_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HPG86808.1"
                     /translation="MDRGYVALVLNAHLPFVRQPDYPSFLEERWLFESLSETYLPLLR
                     VFARLEADRIPWKLNLAVSPTLEAMLGDSMLQQRYVDWLGAQIELAEREVARCADDAA
                     FRPLAAMYHELYTKNREDFSVLYAGNIVGALDFYFKKGRIDLMTSGATNAFMPIYRDY
                     PEAIAAQIEAAVVSFRAAFGRSPSGFWMPQLGWYPGVEEPLSAYGLQYTVVSTRGALL
                     GDPTPRMGSYAPVSCPNGFTAFIRDVAATDAVWSETNGFPADPVYRDFYRDIGFDLPL
                     DYIGPYVEQQQIRTFTGFKYWAITGPGDRKVPYDAERASLRVEDHVESFLRDRVLQAK
                     AAAAHMGGREPLMVAPYDAELFGHWWYEGPQWIETLFRLAHHTDELQFVTLSEYMRAN
                     PENFESVPEFTSWGDGGYGGVWLEKSNDWIYRHVFKMIERMTELAERFPNESGLRERV
                     LNQAAREVLLAQASDWPFLLRAGKSGSFARKQIEDAVTNFNRIYEMLCANTVGTEWLT
                     RLEKRNNIFPSINYRVFRRKR"
     gene            1765..2229
                     /gene="ybaK"
                     /locus_tag="PLQ29_08940"
     CDS             1765..2229
                     /gene="ybaK"
                     /locus_tag="PLQ29_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HPG86809.1"
                     /translation="MTTNALRLVRSMGISVEAFEYEVDESDLSAVSVASRLGLDPDRV
                     FKTIAVRGAKVGPFLCVVPGSTEVDLKKAAKAAGDKSAEPLPLRELEPLTGYVRGGCS
                     PVGTRTPLPVFIDETAALWETISVSAGARGLQMVLAPDDLARASGAEFADLT"
     gene            complement(2401..>2926)
                     /locus_tag="PLQ29_08945"
     CDS             complement(2401..>2926)
                     /locus_tag="PLQ29_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86810.1"
                     /translation="AFSMLDELLFSIVRTDHGACYSVWSRAFGFKSPYGSLVVYKTDR
                     PSEAKGWVDEAIALLASGKTINIKGGDEKYAPIASTIEAYKAKYVNAFFGSQQTNAET
                     AAQLASSHIYFGDHLEYLRFIDKIDAISAEDVVAAVRAYVVDAPISWIVVGDQATLSK
                     VDKTKFDAFTGTVE"
BASE COUNT          517 a          941 c          922 g          546 t
ORIGIN      
        1 cgtcaggacg ccgagggcgt tgatggccga ttcactcgcc aagctcgacg ccgacgccgc
       61 gacgctggct acgctgtccg gattggctag cctcgagata gcgccagcgc cggagcacca
      121 tccttcccgc atcctcggcg ggttcgacga ataacggagg agccccgtgg accgcggata
      181 cgtagcgctc gttctaaacg cacacctgcc gttcgttcgg caacccgatt acccgagctt
      241 tctagaggaa cgctggttgt tcgagtcgct gtcggagacc tacctgccgc tcctgcgggt
      301 cttcgccagg ctcgaggccg acagaatccc atggaagctc aacctggcgg tttcgccgac
      361 cctcgaggct atgctcggcg actccatgct gcagcagcgc tacgtcgact ggctcggcgc
      421 gcagatagag ctggccgaga gggaagtggc gcgatgcgcc gacgacgccg ccttcaggcc
      481 gttggcggcc atgtaccatg agctgtacac gaagaaccgc gaggacttct ccgtactcta
      541 tgccggaaac atcgtcggcg ccctcgactt ctacttcaag aaggggcgta tcgacctgat
      601 gaccagcggc gccacgaacg ccttcatgcc gatctataga gactatcctg aggccatcgc
      661 tgcccagatc gaggccgcgg tcgtatcgtt ccgggccgcg ttcggccgct ctccgtccgg
      721 cttctggatg cctcagctcg gctggtaccc tggcgtggag gagccgctga gcgcgtacgg
      781 cctccagtac accgttgtct cgacccgggg ggccctgctc ggggatccta cgccgcggat
      841 gggctcgtac gcgccggttt cctgcccgaa cggcttcacg gccttcatcc gcgacgtggc
      901 ggccaccgac gccgtctggt cggagacgaa cggctttccg gcggatccgg tatatcgcga
      961 cttctacagg gacatcggtt tcgacctgcc gctcgactat atcgggccgt acgtcgagca
     1021 gcagcagatc cgtaccttca ccggcttcaa gtactgggcc atcaccgggc ccggcgaccg
     1081 gaaggttccg tacgacgccg agcgcgcctc gctccgcgtc gaggaccacg tcgagagctt
     1141 cctgcgcgac cgcgtcctac aggccaaggc ggccgccgcc cacatggggg gccgcgagcc
     1201 gctgatggtc gctccgtacg acgccgagct gttcggccat tggtggtacg agggaccgca
     1261 gtggatagag acgctgttca ggctggccca ccacaccgac gagctacagt tcgtcacgct
     1321 gtcggagtat atgcgggcga accccgagaa cttcgagtca gttcccgagt tcacgtcgtg
     1381 gggcgacggc ggctacggcg gcgtctggct cgagaagtcg aacgactgga tctatcggca
     1441 tgtgttcaag atgatcgagc gcatgaccga gctcgccgag cgctttccga acgagagcgg
     1501 gttgcgcgag cgcgtgctca accaggcggc tcgcgaggtg ttgctggccc aggcctcgga
     1561 ttggcccttc ctgctgcgag ccggcaagtc cggctccttc gcgaggaagc agatcgagga
     1621 cgcggtgacc aattttaacc gtatctacga gatgctctgc gccaacaccg tcggcaccga
     1681 atggctgacc aggctcgaga agcgcaataa catctttccg agtatcaact accgggtctt
     1741 ccggcgtaaa cgctaggtga acccgtgacg accaacgccc tcaggctggt ccgatccatg
     1801 ggcatcagtg tcgaggcctt cgagtacgag gtcgacgagt ccgacctgtc ggccgtatcg
     1861 gtcgcctcga ggctcggcct tgaccccgac cgcgtgttca agacgatagc cgtccgcgga
     1921 gccaaggtcg gacccttcct ctgcgtggtg ccgggctcga ccgaagtcga cctcaagaag
     1981 gcggccaagg ccgccggcga caaatcggcg gagccgctgc cgctcaggga actcgagcca
     2041 ctgactggct acgttcgcgg cggctgctcg cccgtaggca ccagaacgcc cctgccggtg
     2101 ttcatcgacg agacggccgc gctatgggag acgatctcgg tcagcgccgg tgcgagggga
     2161 ctgcaaatgg tgctggctcc ggacgacctc gcgcgcgcct ccggcgccga gttcgccgac
     2221 cttacctgag cttttagttt cgaattgaac catggagacg cggagacacg gagaaagcga
     2281 aggaataagg cgagattaag aaaacaagga gattataatc cttgcgtttc tcattctcta
     2341 ttctcctctt gtattctccg cacctccgcg cctccgtggt ctgattgccc tattctcgac
     2401 ttactccacg gtgcctgtga aggcgtcgaa tttggtctta tcgactttgg acagcgtcgc
     2461 ctggtcgccc acgactatcc agctgatagg cgcgtccacg acgtaggcgc gtacggccgc
     2521 gacgacgtcc tccgcgctga tcgcgtcgat cttgtcgatg aagcgcaggt actcgaggtg
     2581 gtcgccgaag tagatgtggc tcgaggccag ctgcgccgcg gtctcggcgt tggtctgctg
     2641 gctaccgaag aacgcgttga cgtacttggc cttgtaggcc tcgatggtcg acgcgatcgg
     2701 cgcgtacttc tcgtcgccac ccttaatatt gatggtcttg ccggacgcca ggagcgctat
     2761 cgcctcgtcg acccagccct tggcctcgct cggcctgtcg gtcttgtaga cgacgagcga
     2821 gccataaggg ctcttgaagc cgaaggcgcg cgaccagacc gagtaacagg cgccgtggtc
     2881 ggtgcggacg atgctgaaca gcagctcgtc gagcatcgag aacgcc
//
LOCUS       DAOPHV010000332         2922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_47497_length_2922_cov_3.437391, whole genome shotgun sequence.
ACCESSION   DAOPHV010000332 DAOPHV010000000
VERSION     DAOPHV010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47497_length_2922_cov_3.437391"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="PLQ29_08950"
     CDS             complement(<1..1079)
                     /locus_tag="PLQ29_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86811.1"
                     /translation="MTGRSTRYAHAPWPAALPAALAAIAVLFSACSGGAPRPEATPAP
                     EPGFQLAPGGTLVLAVDASGGLYPLPPPETGSVPAEPVSEPFPIAVSAATIQPIVGGA
                     VVAINRKGLSRLTIRRSAPDVAASVLIEPIDGAAAEFDGRTVAPSWGSGGDAVFLLYR
                     HPVYELAERRSPASVTIAATLDRARIVRPGLGDDAFAAYPRAPGEWLAQYRTETTERV
                     TTAYARLSLESGEPETIRTSPVTREDFERLVAPEPMERAPEALRAAVAAIGGPVLLEA
                     RLPDGSRRAFVRGDPGQAAPAWGHLYGDRACVATDDWRVVLADGASGVIVDPSPVPPY
                     PGATVRDAVLLDDALILLWEAGSFP"
     gene            complement(1173..2660)
                     /gene="proS"
                     /locus_tag="PLQ29_08955"
     CDS             complement(1173..2660)
                     /gene="proS"
                     /locus_tag="PLQ29_08955"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPG86812.1"
                     /translation="MAEKITPRSVDYSQWYIDIVLNAKLADYSPVKGCMVIRPRGYAI
                     WERLQAELDRRFKATGHQNAYFPMFIPMSFLKKEAEHVEGFKPELAVVTHAGGEELEE
                     PLAVRPTSETIIWSKYRDWIQSWRDLPLLYNQWANVVRWEKRTRLFLRTTEFLWQEGH
                     TAHETKDEALKETETMLEVYRAFAEEYLCLPVVAGRKSDAEKFAGAVATYSIEAMMQD
                     KKALQAGTSHFLGQNFAKAFEVQYQTREGGLDYVWATSWGVSTRLLGAIIMTHSDDKG
                     LVLPPSLASEEIVVVPIFKTDNKAAVLEYAGKVVDALKADGRRVVFDTDDGSSPGWKF
                     AEWEMRGVPIRIEAGPKDAANGTVVMVRRDTGEKTFVKVDEVPAKARETLAAMQKDLL
                     EKARAFQDANTKDVADRDELVRFFGKEKGKLDADGELHEGTVEAPGGFARGLWCGGRD
                     CEAQLKADLKVTIRNLPFGQQDGCDGACCLCGEPAKHRAIFARSY"
BASE COUNT          437 a          956 c         1020 g          509 t
ORIGIN      
        1 tccgggaagg agccggcctc ccagagtagg atcagggcgt cgtcgagcag gaccgcgtcc
       61 ctaacggtcg cgcccggata gggcggtacg ggggaggggt cgacgatgac gcccgaggct
      121 ccgtcggcta gcacgacgcg ccagtcgtcg gtggcgacgc aggcgcggtc gccgtataga
      181 tggccccagg ccggcgcggc ttggcctggg tcgccgcgga cgaaggccct tcgcgagccg
      241 tcgggcaggc gagcctcgag caggacgggc ccgccaatgg cggcgacggc cgctcgcaac
      301 gcctcgggcg ctcgttccat gggttcgggg gcgacaaggc gctcgaagtc ctcccgggtc
      361 acggggcttg tccgtatcgt ctccggttcg cccgattcga ggctcagcct ggcgtaggcc
      421 gtcgtgaccc gttccgtggt ctccgtgcgg tattgggcca gccactcgcc aggagcgcgg
      481 ggatacgcgg cgaaggcgtc gtcgccgagg cccggtcgaa cgatcctcgc tcgatcgagc
      541 gtggcggcta tagtcaccga agcgggcgac cgccgctcgg ccagctcgta gaccgggtgc
      601 cggtagagga ggaagacggc gtcaccgccc gagccccagg acggtgcgac ggttcgcccg
      661 tcgaattcgg ccgccgcgcc gtcgatcggc tcgatcagga cgctagcggc gacgtcgggg
      721 gccgagcgcc ggatcgtcag gcgactgaga cctttccggt ttatggcgac gacggcgccg
      781 ccgacgatcg gctgtatcgt cgccgcggag accgcgatcg ggaagggttc cgagaccggc
      841 tcggcaggaa ccgaaccggt ctcgggcggg ggcagcggat acaagccacc cgaagcgtcc
      901 accgccagca cgagcgtccc gccgggagct agctggaagc cgggctcggg tgccggagta
      961 gcctcgggcc gcggcgctcc gcccgagcag gcggagaata gcacggctat tgcagcgagg
     1021 gccgcgggta gcgcggcggg ccatggtgcg tgggcatagc gagtcgatcg tccggtcatg
     1081 gcgccagtct agcaggagaa cggcgatgcc ggaagacgaa aacgccgccc cgcgacgggc
     1141 gcggggcggc ggcttggtcc gtagggccgg tctcagtacg accgtgcgaa gattgcgcgg
     1201 tgcttggccg gctcgccgca gaggcagcag gcgccgtcgc aaccgtcctg ctggccgaac
     1261 ggcaggttgc gtatcgtcac cttcaggtcg gccttgagct gggcctcgca gtctctgccg
     1321 ccgcaccata ggccgcgggc gaatccgccg ggagcctcga cggtgccttc gtgcagctcg
     1381 ccgtcggcgt cgagcttgcc tttctccttg ccgaagaacc gtacgagctc gtcgcgatcg
     1441 gcgacgtcct tcgtattggc gtcctggaag gccctggcct tctccaggag gtccttctgc
     1501 atcgcggcga gggtctcgcg cgcctttgcc ggcacttcat cgaccttgac gaaggtcttc
     1561 tccccggtgt cgcggcgcac catcacgacg gtgccgttgg ccgcgtcctt ggggccggcc
     1621 tcgatacgga taggcacgcc gcgcatctcc cactcggcga acttccagcc ggggctcgag
     1681 ccatcgtcgg tatcgaagac cacgcgtcgg ccgtcggcct tgagcgcgtc gacgaccttg
     1741 cccgcgtatt cgagcacggc ggccttgttg tcggtcttga agatcggcac gacgacgatc
     1801 tcctcgctgg ccagcgacgg cggcagcacg aggcccttgt cgtcggagtg cgtcatgatg
     1861 atggcgccga gcagcctcgt cgacacgccc cagctcgtcg cccagacgta gtcgaggccg
     1921 ccttcgcggg tctggtactg tacctcgaag gccttggcga aattctggcc gaggaagtgg
     1981 ctggtgccgg cctgcagggc tttcttgtcc tgcatcatcg cctcgatgga atacgtggcg
     2041 accgcgccgg cgaatttctc cgcgtcgctc ttgcgaccgg ccacgaccgg caggcagagg
     2101 tactcctcgg cgaaggcccg gtagacctcg agcatcgtct cggtctcctt gagcgcctcg
     2161 tccttcgtct cgtgagccgt atggccttcc tgccagagga actcggtggt gcggaggaag
     2221 agcctggtac gcttctccca gcggacaacg ttggcccact ggttatagag caggggcaga
     2281 tcacgccagg actggatcca gtcgcggtac ttgctccaaa taatcgtctc gctcgtgggc
     2341 ctgacggcga gaggctcctc gagctcctcg ccgcccgcgt gcgtcacgac cgccagctcc
     2401 ggtttgaagc cttccacgtg ctccgcttcc tttttgagga agctcatggg tatgaacatg
     2461 gggaaatacg cgttctggtg ccccgtcgcc ttgaagcggc ggtcgagctc ggcctggagg
     2521 cgctcccaga tcgcgtagcc gcgcggcctg atgaccatgc accccttgac cggcgagtag
     2581 tccgccagct tcgcgttgag tacgatgtcg atataccact gagagtagtc gacgctccgg
     2641 ggggtgatct tttcggccat ggctgtacct cgcaaggggc ttggcggcct cgagcggccg
     2701 ccgcgtttac gccgcgaggc gccggcccgc aatggacccg cgcctcgcgg tctggatgat
     2761 tctatacgat atggcgccgc ctgttcaacg gcgacggagg cttacggtaa cctgaaggtc
     2821 tcgaccatat aatgtacgga cgcgccatcg gcgcttttct cgagcttctc tatgatcatg
     2881 acgacggttt tgccgtccgg ggcgactatt atctcgcgga tg
//
LOCUS       DAOPHV010000333         2912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_47717_length_2912_cov_5.069303, whole genome shotgun sequence.
ACCESSION   DAOPHV010000333 DAOPHV010000000
VERSION     DAOPHV010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47717_length_2912_cov_5.069303"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..744
                     /locus_tag="PLQ29_08960"
     CDS             103..744
                     /locus_tag="PLQ29_08960"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HPG86813.1"
                     /translation="MRCWSCRRPKALCLCPSEPPMPTRTRVVLLMHPMEWKHEKCTTG
                     RLTCLNLRNSEIIPGLRFGDDPRYRALVDDPANFPVLLYPGKDAFDLSSGGFPAAELG
                     DRRLVVFLIDSTWACAKAILRESPCLRALPCVKFTPTVPSRYVIKRQPDPLCLSTIEA
                     THELLLALENAGLDAYPDKERLLRSFFAMRDYQLERIADDRNPRRKKKWDRPS"
     gene            complement(737..2467)
                     /locus_tag="PLQ29_08965"
     CDS             complement(737..2467)
                     /locus_tag="PLQ29_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG86814.1"
                     /translation="MLKRFLPYYKPYLGLLALDLACALGLGLADLVFPAATRRVVDVI
                     VPAGDMAGLARFGLLLVALYAVRAGLEFILGYYGHVLGVNIQHDLRRDIYRHLHTLDT
                     RFFDDTKTGQIMSRIVGDLFDLAEISHHLPEDLIMAGVRLIGSLVVMLAIDWRLALVV
                     FAIVPAQVLFSSTFRARFRRAFRRNKETMAVINEHIEESVSGVRVVRAFGNEDYERAR
                     FDDGNATFRSSRIESVFHIGVFNTGVSFLSNLGLVIVLVAGGYAAWRGSVSVGAYVAF
                     ALYVARFFQPIEVLVRSMEQFQEGAAGFGRFLEIVDTKPTIVDAPGAIELASVRGDVE
                     FREVSFRYGDGLERVLERVDLRVAAGETVAIVGPSGAGKTTLCSLIPRFYDIEEGSIT
                     VDGLDVREATIASLRQAVGLVQQDVFLFSGSVRDNIAYGRLGASDEEVEAAAAAANAL
                     EFIRSLPLGLDTLVGERGVKLSGGQKQRISIARMFLKDPPILILDEATSSLDTMSELA
                     IRESLDRLSRGRTTFIIAHRLATIRHAARILVLTEDGVVEQGRHDDLVAAGGVYKRLY
                     DAQVESLLVS"
     gene            2515..>2912
                     /locus_tag="PLQ29_08970"
     CDS             2515..>2912
                     /locus_tag="PLQ29_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86815.1"
                     /translation="MILSEPSAPTADDLREREKELDFFYSLAALLSKPGLDADEAAAG
                     CARLFAAAMGRPEAARVVVRLGDRTATYAGEGAPSDSPDSSDSHDSDGPSPDAMAALA
                     GSRLACGVEASYPGGEQAFSERERSLCSSTA"
BASE COUNT          491 a          990 c          983 g          448 t
ORIGIN      
        1 tgcatggtgg cgttaccggc cgccgtcagc tcataaatct gccatggctg gaccagcgtt
       61 tttttgcaga gatcggcaat gagctgattc aggcagtcca ctattcgatg ctggagctgc
      121 cgcaggccca aggcgctgtg tctgtgtccg tccgagccgc cgatgcctac gcggacccgc
      181 gtcgtcctcc tcatgcaccc gatggaatgg aagcatgaga agtgcacgac cggcaggctg
      241 acctgcctga acctacggaa cagcgagatc ataccgggcc tacgcttcgg ggacgatcca
      301 cggtaccggg ccctggtcga cgacccggcc aacttccccg tgttgctcta ccccggcaag
      361 gacgctttcg acctatcctc gggcggcttc cccgcggccg agctgggcga ccgccgcctg
      421 gtcgtgttcc tgatcgactc gacctgggcc tgcgccaagg ccatactccg cgagagcccc
      481 tgcctccgcg ccctaccctg cgtcaagttc acgcccaccg tcccaagccg ctacgtcatt
      541 aaacgccagc ccgaccccct gtgcctgtcg acgatagagg cgacgcacga gctgctcctg
      601 gcgctggaaa acgccgggct cgacgcgtac cccgacaagg agcgcctgct gcggtcgttc
      661 ttcgcgatgc gcgactacca gctcgagcgc atagccgacg accgtaaccc gcggcgcaag
      721 aagaagtggg accgccctag ctgacgagga ggctctccac ctgcgcgtca tacaggcgct
      781 tgtagacgcc gccggcggcc accaggtcgt cgtgccggcc ctgttcgacg acgccgtcct
      841 cggtcagcac gagtatgcgc gcggcgtggc gtatcgtcgc caggcggtgc gcgatgatga
      901 acgtggtgcg gcctcgggac agccggtcga gcgactcgcg tatggccagc tcgctcatcg
      961 tgtcgagcga gctcgtcgcc tcgtcgagga tgaggatagg cggatccttg aggaacatgc
     1021 gggctatcga gatgcgctgc ttctggccgc ctgacagctt gacgccgcgc tcgccgacca
     1081 gcgtatccag gcccaggggg agggagcgta tgaattcgag cgcgttggcc gcggccgcgg
     1141 cggcctcgac ctcctcgtcg ctcgcgccca ggcggccgta ggcgatgttg tcccggacgc
     1201 tgccggagaa caggaacacg tcctgctgca cgaggccgac cgcctgcctg agcgaggcta
     1261 tcgtcgcctc ccgtacgtcc agcccgtcga ccgtgatcga gccctcctcg atgtcgtaga
     1321 agcgtggtat cagcgagcag agcgtcgtct tacccgcgcc agaagggccg acaatcgcga
     1381 ccgtctcgcc ggccgccacg cgcagatcga cgcgttccag cacccgctcg aggccgtcgc
     1441 cgtagcggaa gctcacctcc cggaactcga cgtcgccgcg gacgctcgcc agctctatcg
     1501 cgccgggggc gtcgacgatg gtcggcttcg tgtcgactat ctcgaggaag cgcccgaagc
     1561 cggccgcgcc ctcctggaac tgctccatcg agcgcacgag cacctcgatg ggctggaaga
     1621 agcgcgccac gtagagcgcg aaggccacgt aggcgccgac gctgacggag ccgcgccagg
     1681 cggcatagcc gccggcgacg agcacgatga ccagcccgag gttggacagg aacgacacgc
     1741 cggtgttgaa cacgccgata tggaagaccg actctatcct cgagctcctg aacgtggcgt
     1801 tgccgtcgtc gaaacgggcg cgctcgtagt cctcgttgcc gaaggcccgc acgacgcgca
     1861 cgcccgagac gctctcctcg atgtgctcgt tgatgacggc catcgtctcc ttgttgcggc
     1921 ggaacgcgcg gcggaagcgc gcgcggaagg tagacgagaa caggacctgg gccggcacga
     1981 tcgcgaagac cacgagcgcc aggcgccagt ctatggcgag catcacgacg agcgagccta
     2041 tgagcctcac gccggccatg atcaggtcct cgggcaggtg gtgcgatatc tcggccaagt
     2101 cgaacaggtc gccgacgatg cgcgacatga tctggccggt cttcgtatcg tcgaaaaagc
     2161 gcgtgtcgag ggtatgcagg tggcggtaga tgtcgcgccg gaggtcgtgc tggatgttca
     2221 cgccgagcac gtggccgtag tagccgagga tgaactcgag cccggcgcgg acggcgtaca
     2281 gcgctacgag caacaggccg aagcgcgcga ggccggccat gtcgccggcc ggtacgatca
     2341 cgtcgacgac gcgccgcgtg gccgccggga agacgagatc ggccagcccg agccccaggg
     2401 cgcaggccag gtcgagggcc aggaggccca gatagggctt gtagtacggg aggaaacgct
     2461 tgagcatgat acgacatggt agcggttcgg gagccttcgt gtatactgga acgcatgatt
     2521 ctatctgaac cgtccgcccc gacggccgac gacctgcgcg agcgcgagaa ggagctggac
     2581 ttcttctatt cgctcgcggc gctcctgtcc aagccgggcc tcgacgccga cgaggcggcc
     2641 gcaggctgcg cgcggctctt cgcggcggcg atgggccggc ccgaggcggc ccgcgtggtc
     2701 gtcaggctcg gcgatcggac ggcgacgtac gctggcgagg gcgcgccgtc cgactcgccc
     2761 gactcgtccg actcgcacga ctccgacggc ccttcgcccg atgccatggc ggcactcgcc
     2821 ggctcgcgcc tcgcctgcgg cgtcgaggcg agctacccgg gcggggagca agcgttctcc
     2881 gagcgcgagc gctcgctgtg ctcgtcgacg gc
//
LOCUS       DAOPHV010000334         2912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_47721_length_2912_cov_3.911446, whole genome shotgun sequence.
ACCESSION   DAOPHV010000334 DAOPHV010000000
VERSION     DAOPHV010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47721_length_2912_cov_3.911446"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1374)
                     /gene="secF"
                     /locus_tag="PLQ29_08975"
     CDS             complement(145..1374)
                     /gene="secF"
                     /locus_tag="PLQ29_08975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPG86816.1"
                     /translation="MKRVINFHKAFLPATLASVVVLVAGLISLATMGLNLGVDFQAGI
                     NQSVRLAYPVADVSYEGPGSPVLSIGAGELSLVFSGADVDSRTVSWNLRTVGTIADLA
                     GLLKAEGVTMAVRDGAGLSADLLVPTYQGDYTLGASPRLVHRAPKSSAEAYGSIEEIR
                     AAVSSLGSVSVQNVGGADSMQYLVRVSDDGSDKSFSETIPGRIRAALESAFGADKVVV
                     IKTDYVGARFSEDLSSSAWKLSLFTALAILAYATIRFKMQYAVGAVLAILHDCLIMIA
                     FMVWTRMEFNTTSIAALLTILGYSINDTIVIFDRVREDRKLDPNAKMSAVIDRSVTET
                     LGRTVITTVTTLLAVAALYFFTSGSIKDFALALIVGIVSGTYSTIFIATGFVHLWDKY
                     GGKRQAKAGKLAAKPAN"
     gene            complement(1371..>2912)
                     /gene="secD"
                     /locus_tag="PLQ29_08980"
     CDS             complement(1371..>2912)
                     /gene="secD"
                     /locus_tag="PLQ29_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPG86817.1"
                     /translation="RLVAASDAEPLPERFDPLVKRARKIYKELGIAAPAAWDAKAFLA
                     AFDNRSDALAEIEDYYRSHVLGLKKLHGSAVQLGLDLSGGMSVVIRADIEALEAKVGR
                     SLSSAERDDAVERALEVLNNRIDKFGLTEPVIRRQGDDRIYVEIPGAADPDRINSIIM
                     GRGKLAFHIVDEQATASLAAYLEANPLAISDDLTLADPSQIPAGTMVRKYYTKDKYGL
                     DEWTGSYMVIAAEAGLDGNHIESATVSSDAVTGQPEVVFSLDKEGGEIFYALTSANVE
                     KTMAVVLDDRVRTGARISGPIQANVKITGFDAEEANNLALILRSAALPVELIVESQQA
                     VGASLGEDTILEGRNAVLLGLALVFLFMLAYYKGAGVNATLAQVLNLFLMFSILSAFN
                     LTLTLPSIAGFVLTIGMAVDANVIIFERIKEELRDGKGRKAAIDAGFAKAFWAVMDGN
                     ITTIIAALFLSQLGTGSIQGFAVSLAIGNISSLFTALFVSKLLFDFNTDVAKHRTVSI
                     GWRVK"
BASE COUNT          519 a          859 c         1030 g          504 t
ORIGIN      
        1 ctcctcggct tccataacgc cccctatcgg gcgctagtat acacgaaacg acgacgactc
       61 gaacgtatcg atcccctaat gcagaaaaag ggccgaaccg cgtgcggttc ggcccgatgg
      121 ctttgagata tgcgacgaac ggactcagtt cgccggcttg gccgccagct ttccggcctt
      181 ggcctgcctc ttgccgccgt acttgtccca gagatgcacg aacccggtgg cgatgaagat
      241 ggtcgaatag gtacccgaga cgatgccgac gatcagcgcc agggcgaagt ccttgatact
      301 gcccgaggtg aagaaataga gcgctgctac ggccagcagg gtcgtgacgg tcgtgatgac
      361 cgtgcggccg agggtctcgg tcacggatcg atcgatgacc gccgacatct tggcgttcgg
      421 gtcgagcttg cgatcctcgc ggacgcggtc gaagatgacg atcgtgtcgt tgatcgagta
      481 gccgagtatc gtgaggagcg cggcgatcga ggtggtgttg aactccatgc gggtccagac
      541 catgaaggct atcataatga gacagtcgtg caggatggcg agcacggcac cgacggcgta
      601 ctgcatcttg aacctgatcg tggcgtacgc gagtatggcg agcgcggtga acaggctgag
      661 cttccaggcg ctcgaggaca ggtcctcgga gaagcgcgcg ccgacgtagt cggtcttgat
      721 gacgacgacc ttgtcggcgc cgaaggccga ctcgagggcg gcgcgtatcc tgccggggat
      781 agtctcgctg aaggacttgt ccgagccgtc gtcgctgacg cggacgaggt actgcatcga
      841 gtcggcgccg ccgacgttct gcaccgacac ggagccgagc gacgaaacgg ccgcgcggat
      901 ctcttcgatc gagccgtagg cctcggccga cgacttgggc gcgcgatgga cgagccgcgg
      961 agaggctccg agggtatagt cgccctgata ggtcgggacg agcaggtcgg cggagaggcc
     1021 tgcgccgtcg cgtacggcca tggtcacgcc ctcggccttg agcaagccgg ccaggtcggc
     1081 gatggtgccg acggtgcgca ggttccagga tacggtcctg gagtcgacgt cggccccgga
     1141 aaagacgagg gatagctcgc cggctccgat ggacagcacg ggcgagccgg gcccttcgta
     1201 gctgacgtcg gcgacgggat acgcgagccg cacggactgg ttgatgcccg cttggaagtc
     1261 cacgcccagg ttgaggccca tcgtcgccag cgagatgagt ccggcgacga gcacgacgac
     1321 cgaggctagg gtcgcgggca ggaacgcctt atggaagttg atgacgcgct tcatttgacc
     1381 ctccagccaa tcgacacggt gcggtgctta gccacgtcgg tgttgaagtc gaataacagc
     1441 ttggacacga acagcgcggt gaacagcgac gaaatgttgc cgatcgcgag gctgacggcg
     1501 aagccctgga tggagccggt gccgagctgg gacaggaaca gggcggcgat gatcgtcgtt
     1561 atgttgccgt ccatgacggc ccagaaggct ttcgcgaagc ccgcgtcgat ggcggcctta
     1621 cggcccttgc cgtcgcgtag ctcctccttg atgcgctcga agatgatcac attcgcgtcg
     1681 accgccatgc ctatcgtcag cacgaaaccg gctatcgacg gcagggtcag cgtcaggttg
     1741 aaggccgaca ggatcgagaa catcaggaac aggttgagca cctgggccag cgtggcgttg
     1801 acgccggcgc ccttatagta cgccagcatg aacaagaaca cgagggccag gccgagcagc
     1861 acggcgttgc ggccctcgag gatggtgtcc tcgccgagcg atgctccgac cgcctgctgg
     1921 gactcgacga tcagctcgac cggcagggcc gccgagcgca ggatcagggc caggttgttc
     1981 gcctcttcgg cgtcgaagcc ggtgatcttg acgttcgcct ggatggggcc ggagatcctg
     2041 gcgccggtac gtacgcggtc gtcgagcacg acggccatgg tcttctcgac gttggcgctg
     2101 gtcagcgcgt agaagatctc gccgccctcc ttatcgaggc tgaagacgac ctcgggctgc
     2161 ccggtaacgg cgtcggatga gacggtggcg ctctcgatat ggttgccgtc gaggccggct
     2221 tccgcggcta tcaccatgta gctaccggtc cactcgtcga ggccgtactt gtccttggtg
     2281 tagtacttgc gcaccatggt gccggccggt atctgagacg ggtcggccag ggtcaggtcg
     2341 tcgctgatcg cgagcggatt agcctcgagg taagcggcga gcgaggcggt cgcctgctcg
     2401 tccacgatgt ggaaggctag cttgccgcga cccatgatga tgctgttgat gcggtcggga
     2461 tcggcggcgc cggggatctc gacgtagatg cggtcgtcgc cctggcggcg gatgaccggc
     2521 tcggtcagac cgaacttatc gatgcggttg ttgagcacct cgagggcgcg ctcgacggcg
     2581 tcgtcgcgct cggccgacga tagggacctg ccgaccttgg cctcgagggc ctcgatgtcg
     2641 gcgcggatga cgaccgacat gccgccggac aggtccaggc cgagctggac ggcgctaccg
     2701 tgcagcttct tgaggccgag cacgtgcgat cggtagtagt cctcgatctc ggcgagggcg
     2761 tcggaacgat tgtcgaaggc ggcgaggaag gccttggcgt cccaggcggc cggggcggcg
     2821 atgccgagct ccttgtagat cttcctggcc cgcttgacga gcgggtcgaa gcgttcgggc
     2881 aacggctcgg cgtccgaggc ggccacgagg cg
//
LOCUS       DAOPHV010000335         2908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_47824_length_2908_cov_3.120224, whole genome shotgun sequence.
ACCESSION   DAOPHV010000335 DAOPHV010000000
VERSION     DAOPHV010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47824_length_2908_cov_3.120224"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..819)
                     /locus_tag="PLQ29_08985"
     CDS             complement(52..819)
                     /locus_tag="PLQ29_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86818.1"
                     /translation="MLKFFLKKSFFDGWDTMFSLAAFNAVLLALAAVGLWLPSLTGYA
                     WLRLAGLAFAVLAASVWACVAANALYRVADGRSLGLDEIRGAVRDGIVPGLQYGAVVT
                     LAVAAASAALPFYASLGGALGVFAAGLSFWLFAALWLTLQYFPAVKAAEGKGFVESMR
                     LSFIAFVDAPGFAAILALDGLLCVALSPVVAFLLPGFSGAILAVCEAYRLRRLKLRWL
                     SNAGARPGRAPWEELLAEDVEAFGNRGFKDLLFPWKR"
     gene            969..2267
                     /locus_tag="PLQ29_08990"
     CDS             969..2267
                     /locus_tag="PLQ29_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPG86819.1"
                     /translation="MKRFVAILLAAALVAPAFAQAPTVSALKGKTVEVVVWTHEDSAR
                     TALEKRLIDEFTKANPGVTVKYVTYPSGKIKDIVVAGFAANAGPDIFNLEIFDQYQFI
                     ANGRVAPVDYKLAGFKSAKDMQAKYMAGMLDPVTEDGKIYGMPLELTNWAVYLNKKIF
                     RDAGLDPMKDYPKTWEEVMAVSEKIVKRDGQIITRRGFDFRYPYYLTSVVPMVEQLGG
                     ELVSKDGKSVVTNDAAWLKVLAYFKDFGPNGKNLGSPTYTAARKVFDNDKNEIAMSLS
                     GLYQEARMRDANPTFYNSGEWMVVPFPQWKDAVRKVPNNYYGHYYMVNAQSSKLEQQA
                     AWALLGYMLQHGEEYLEKVAIVQPTKALFESRIFKETPYSKVFEDDMAAGHIVYYGAA
                     SSKIESLLKEAFESVMLSGVEPAKALDKLRRDVKAALEDI"
     gene            2447..>2908
                     /locus_tag="PLQ29_08995"
     CDS             2447..>2908
                     /locus_tag="PLQ29_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPG86820.1"
                     /translation="MKRRESASGLERNRARWGWVFVAPGLLFFSVFSFYPMLNAIWTS
                     FFNKRLLSLKAPKFIGLQNYQRVLGSEEFWNSARATGVFTVGVFVPLFCLSLLFAALI
                     TQMRRGKRLAQAALYSPAVLSSVVAALIWLLMLDPRGLANQFVNWVSSTPGI"
BASE COUNT          584 a          919 c          932 g          473 t
ORIGIN      
        1 caggggaacc tgacgaacag gcacggcgcg agcgcttacg tgaaagcccg ctcagcgctt
       61 ccaggggaac aggagatcct tgaagccgcg gttcccgaag gcctcgacgt cctcggcgag
      121 cagctcctcc caaggagcgc ggcccggcct ggcgccggca ttcgatagcc agcgtagctt
      181 gaggcgcctg agccggtagg cctcgcagac ggccaggatc gcgccggaga agcccggcag
      241 caggaaggcc acgacgggcg acagagccac gcagagaagc ccgtccaggg ccaggatagc
      301 ggcgaaccct ggcgcgtcga cgaaggcgat gaacgaaagg cgcatcgact cgacgaagcc
      361 cttgccctcg gccgccttga cggctgggaa gtactgcagg gtcagccaga gcgccgcgaa
      421 cagccagaac gagagcccgg ctgcgaagac gccgagcgcg ccgccgaggc tcgcgtagaa
      481 cggtagcgcg gccgaggccg ccgcgaccgc cagcgtcacc accgcgccgt actgtaggcc
      541 gggtacgatc ccgtctcgga cggccccacg gatctcgtcg aggccgagcg agcgcccgtc
      601 ggcgacccgg tatagtgcgt tggcggctac gcaggcccag accgaggctg ccaggaccgc
      661 gaaggccaga ccggccagcc tgagccaggc gtaaccggtc aaggacggca gccagaggcc
      721 gacggcggcc agggcgagca gaacggcgtt gaaggcggcc agggagaaca tggtgtccca
      781 gccgtcgaag aagcttttct tcaggaagaa cttgagcatg gacaagatat accagggccg
      841 tcaggctctg gctagcccga cgccgcccaa gggcgccgtc tttccaaaca aaatgccggg
      901 atagcggcat tgcgggttcc gcgggatgga tcgatcctag tctcaatagt tccgtagagg
      961 aggatgccat gaaacgtttc gtagcgatac tgctggcggc cgcgctcgta gcgccggcct
     1021 tcgcgcaggc cccgaccgtc tccgccctga agggcaaaac agtcgaggtc gtcgtctgga
     1081 cccacgagga ttccgccagg acggcgctcg agaagcgcct gatagacgag ttcaccaagg
     1141 ccaacccggg cgtaaccgtc aagtacgtga cctatccgtc cggcaagatc aaggacatcg
     1201 tcgtcgctgg cttcgcggcg aacgccggtc ccgacatctt caacctcgag atattcgatc
     1261 agtatcagtt catagccaac ggtcgcgtgg cgccggtcga ctacaaactg gccggcttca
     1321 agagcgccaa ggacatgcag gccaagtaca tggccggcat gctcgacccg gtcaccgagg
     1381 atggcaagat ctatggcatg cccctcgaac tgaccaactg ggccgtgtac ctgaacaaga
     1441 agatcttccg cgacgccggg ctcgacccga tgaaggacta tcccaagacc tgggaagagg
     1501 tcatggccgt ttccgagaag atcgtcaaac gcgacggcca gatcatcacc cgccgcggct
     1561 tcgacttccg ctatccgtac tacctgacct cggtcgtgcc catggtcgag cagctcggcg
     1621 gcgagctcgt gtccaaggac ggcaagtcgg tcgtcacgaa cgacgcggcc tggctcaagg
     1681 tcctcgcgta cttcaaggat ttcggaccca acggcaagaa cctcggttca ccgacctaca
     1741 cggctgcgcg aaaggtcttc gacaacgaca agaacgagat agccatgtcg ctgtccggcc
     1801 tgtaccagga agcccgcatg cgcgacgcca acccgacgtt ctataactcg ggcgagtgga
     1861 tggtcgtgcc cttcccgcaa tggaaggacg ccgtgcgcaa ggtgccgaac aactactacg
     1921 gccactacta catggtcaac gcccagtcgt ccaagctgga gcagcaggcc gcctgggccc
     1981 tactcggcta catgctgcag cacggcgagg aatacctcga gaaggtggcc atcgtccagc
     2041 cgaccaaggc gctcttcgag tcgaggatct tcaaggagac tccgtattcc aaggtgttcg
     2101 aggacgacat ggccgccggc cacatcgtct actacggcgc ggcgagctcc aagatcgagt
     2161 cactgctcaa ggaagccttc gagtcggtga tgctgtccgg cgtcgagccg gccaaggccc
     2221 tcgacaagct caggcgcgac gtcaaggccg ccctggaaga tatctgacgt agtatcgata
     2281 cttgagccat ggaggcacgg agacacggac taggagtggg cggggggggt aggagaatgg
     2341 aacggatatc cacgttctat ctttgattct ccccctatcg tagtcaccgt gccccgagcc
     2401 tccgtggtag gttgttacag cgcaagtttt ccgttggagg taacgaatga agcgtcgcga
     2461 gtccgctagc gggctggagc ggaacagggc gcgttggggt tgggtgttcg tggcgcccgg
     2521 gttgctcttc ttctcggtct tctcgttcta tccgatgctg aacgccatct ggaccagctt
     2581 cttcaataag cggctcctgt cgctcaaggc cccgaaattc atagggctcc agaactatca
     2641 gcgggtgctc ggctcggagg aattctggaa ttcggcccgc gccacgggcg tcttcacggt
     2701 cggcgtcttc gtgccgctgt tctgtctgag cctgctgttc gccgcgctga tcacccagat
     2761 gcggcgcggc aagcggctgg cccaggccgc gctgtactcg cccgccgtgc tgtcgtcggt
     2821 cgtcgcggcc ctgatctggc tgctgatgct cgacccgcgg ggcctggcca accagttcgt
     2881 caactgggta tcgtcgacgc cgggcata
//
LOCUS       DAOPHV010000336         2906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_47862_length_2906_cov_3.013679, whole genome shotgun sequence.
ACCESSION   DAOPHV010000336 DAOPHV010000000
VERSION     DAOPHV010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47862_length_2906_cov_3.013679"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..>292)
                     /locus_tag="PLQ29_09000"
     CDS             complement(152..>292)
                     /locus_tag="PLQ29_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPG86821.1"
                     /translation="SIEIVRYGKVRRAKLYYIREKIGKKAKIKEQLGGRKPPVRSQAV
                     QG"
     assembly_gap    293..302
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<303..593)
                     /gene="rplS"
                     /locus_tag="PLQ29_09005"
     CDS             complement(<303..593)
                     /gene="rplS"
                     /locus_tag="PLQ29_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPG86822.1"
                     /translation="MNLIKKIEAEQTKAYQDVKAGDTVRVHFKIVEGKTERIQIYEGL
                     VIAMKNAGIGQTLTVRKNSYGVGVERVFPMHSPRIEKIEILRIPVYNQPGETI"
     gene            complement(590..1321)
                     /gene="trmD"
                     /locus_tag="PLQ29_09010"
     CDS             complement(590..1321)
                     /gene="trmD"
                     /locus_tag="PLQ29_09010"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPG86823.1"
                     /translation="MRLDVLTLFPGILEGFFTSSIMARAVERGLVDYSLTDIREFATD
                     KHHKCDDELFGGGAGMLMMPGPVSAALESVGARESRVVYVTPGGRLFNQAYARELASE
                     EKLVILCGRYEGVDQRVIDRYVTDEISIGDYVLSSGETAALVIVDAIYRLIPGVITGE
                     SLDEESFEGGLLEYPQYTRPAVYDTMVVPDVLVSGHHANIVKWRMKKRLEKTLLYRPD
                     LLQGKGLEGELRAMILELIGQGGEA"
     gene            complement(1335..1835)
                     /gene="rimM"
                     /locus_tag="PLQ29_09015"
     CDS             complement(1335..1835)
                     /gene="rimM"
                     /locus_tag="PLQ29_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPG86824.1"
                     /translation="MDLLALGRVGKPHGVDGRLRLVSYSGETDHLMRLTEATLRGDGR
                     ELFVRIESVTAHGNGVLMKVVGYDSPERAVALSGMELWAPRDMAAPLDDDQYYYADLV
                     GCALVAGGTELASVVAVCDSGGGDLLEVAKPDGSSAYVPFRKEFVGEVDVHARRIELV
                     APWILE"
     gene            complement(1838..2071)
                     /locus_tag="PLQ29_09020"
     CDS             complement(1838..2071)
                     /locus_tag="PLQ29_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPG86825.1"
                     /translation="MERDLIEYIAKSLVDDPAAVSVNLVETDEGTVVELRVAQDDIGK
                     VIGKRGRIAKSIRTLLSAAATRGGKDAKLLILD"
     gene            complement(2176..2427)
                     /gene="rpsP"
                     /locus_tag="PLQ29_09025"
     CDS             complement(2176..2427)
                     /gene="rpsP"
                     /locus_tag="PLQ29_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPG86826.1"
                     /translation="MSVKIRLKKFGAKRRPYYRIVVLDSRQPRDGKTIDELGIYHPVE
                     KEGAQLRIDEEKARAWLAKGALPTDTVRSLLNKQNIRLG"
     gene            2637..>2906
                     /locus_tag="PLQ29_09030"
     CDS             2637..>2906
                     /locus_tag="PLQ29_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HPG86827.1"
                     /translation="MQPLPPRPLMLAPMVELSHRPLRELIESFGGCDRYYTEMTSAAG
                     YLANSPYDRFFVDPEPRPERTAVQFYGAEVEPIAAAAARLLGKRTA"
BASE COUNT          497 a          917 c          915 g          567 t
ORIGIN      
        1 ccccctgaac ggagcgaggc acgtcggcct gggcgattct cgggacggtt tttcttaaaa
       61 aacgaacgtc ggccgatgac gcatcgccat cgccgacgtt cgttgtatgc ggagcggtcg
      121 acctgtcgtt aggaccgacc ggtcgcaagg atcagccctg gaccgcctgg gagcggacgg
      181 gaggcttgcg gccgccaagc tgctccttga ttttggcttt cttgccgatc ttctcgcgga
      241 tgtagtacag cttggcgcgg cggaccttgc cgtagcgcac gatttcgatg ctnnnnnnnn
      301 nnaatggttt cgccaggttg gttatagact gggatgcgga gaatttctat cttctcgatg
      361 cggggcgagt gcatggggaa cacgcgctcg acgccgacgc cgtatgagtt cttgcgtacg
      421 gtcagcgtct ggccgatgcc ggcgttcttc atggcgatca cgagcccttc gtagatctgg
      481 atacgctcgg tcttgccctc gacgatcttg aagtgcacgc ggaccgtatc gccggccttg
      541 acgtcctgat aggccttggt ctgctcggcc tcgatcttct ttatgaggtt catgcctctc
      601 ccccttgtcc aattaattcc agtatcatcg ccctgagttc accctcgagt cccttgccct
      661 ggagcaggtc gggacggtac aagagagtct tctcgagccg tttcttcatg cgccacttga
      721 cgatgttggc atggtggccc gagaccagaa cgtcagggac taccatggta tcataaaccg
      781 ccgggcgtgt atactggggg tactcgagca aaccgccctc gaagctctcc tcgtcgagcg
      841 actcgccggt gatgactccc ggaatgagcc tgtagatcgc gtcgacgatg acgagggcgg
      901 cggtctcccc cgacgacagc acgtaatcgc cgatgcttat ctcgtcggtc acgtagcggt
      961 ctataacacg ctggtcgaca ccctcgtaac ggccgcagag tatgacgagc ttctcctcgg
     1021 aagccagctc gcgagcgtag gcctgattga acaagcgacc gcccggagtc acatacacga
     1081 cccgcgactc acgagcgccg accgattcga gcgcggcgga gaccggcccg ggcatcatca
     1141 gcatgccggc gccgccgccg aacagctcgt catcgcattt atggtgctta tcggtcgcga
     1201 actcgcggat gtcggtcaag gagtagtcga cgaggccacg ctcgacggcg cgggccatga
     1261 tcgagctcgt gaagaagcct tcgaggatgc cgggaaatag cgtcaggaca tcgagcctca
     1321 tccgcgacct tgccttactc gagtatccat ggggctacca gctctattcg cctggcgtgg
     1381 acgtcgacct cgccgacgaa ttccttgcgg aacggcacgt aggccgagga gccgtcgggc
     1441 ttggcgactt cgagcaggtc gccgccgccg gagtcgcaga cggctacgac cgatgcgagt
     1501 tccgtcccgc cggcgaccag cgcgcagccg acgagatcgg cgtagtagta ctggtcatcg
     1561 tcgagcgggg ccgccatgtc gcggggcgcc catagttcca tccccgacaa agccacggcc
     1621 cgctccggcg agtcgtagcc gactaccttc atgagcacgc cgttgccgtg ggccgttacg
     1681 gactcgatgc gaacgaacag ttcgcggccg tcgccgcgca gggtagcctc ggtaagacgc
     1741 atgaggtgat ccgtctcgcc ggagtaggat acgaggcgga ggcggccgtc gacgccatgg
     1801 ggcttgccga cgcggccgag cgcgaggagg tccaccgtca gtcgaggata aggagcttgg
     1861 cgtccttacc gccgcgcgtc gcggccgcgg acagcagggt gcggatagac ttggcgatgc
     1921 ggccgcgctt tccgatgacc ttgccgatgt cgtcctgcgc gacccgcagc tcgacgacgg
     1981 taccctcgtc ggtctcgacg agattgacgg agaccgccgc ggggtcgtcc acgagagact
     2041 tggctatata ctcgatgaga tctcgttcca ttccttggcc ctttcgtccg tcgggttcgc
     2101 caggatcacc ggctatgaaa cccgcctcga ggcttggcgc cggggcctcg ggcgtcctta
     2161 ggacgcccgt accctttagc cgagacggat gttctgcttg ttaaggaggc tgcggaccgt
     2221 atcggtcggg agagcgccct tggcgagcca agctcgggcc ttctcctcgt cgatgcggag
     2281 ctgagcgcct tccttctcga ccgggtggta gatgccgagc tcgtcgatgg tcttgccatc
     2341 gcgcggctgg cggctatcga gcaccacgat gcgataatac gggcgcctct tggcgccgaa
     2401 tttcttgagc ctgatcttta cgctcacgaa ttcctcctga aatattgccg gaacgtcgat
     2461 ttcggttatt gggacgcggt ataaaaccgc gagccgcgcc gccgatcgat aggcgaccgc
     2521 gacgcggaag cgccgatact cgcgccttgg gtttataccg cataagtcgt ggcagagtca
     2581 atcgtcgggc ggatgaccgc ttgctatcgg ccaagacggc ggcgtatagt gcgccgatgc
     2641 agccactccc gccccgcccc ctgatgctcg ccccgatggt cgagctgagc caccgccccc
     2701 tccgcgagct catcgagtcg ttcgggggat gcgatcgata ctacaccgag atgaccagcg
     2761 ccgcgggtta cctggccaac tccccttacg accgcttctt cgtcgatccg gagccgcgtc
     2821 ccgaacgtac cgccgtccag ttctatggcg ccgaggtcga gccgatcgcc gcggccgccg
     2881 cgcggctcct gggcaagagg acggcc
//
LOCUS       DAOPHV010000337         2899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_48030_length_2899_cov_3.659634, whole genome shotgun sequence.
ACCESSION   DAOPHV010000337 DAOPHV010000000
VERSION     DAOPHV010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48030_length_2899_cov_3.659634"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2322)
                     /locus_tag="PLQ29_09035"
     CDS             complement(<1..2322)
                     /locus_tag="PLQ29_09035"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPG86828.1"
                     /translation="MKAVELAKAYDPKEFEDRVYEAWQAEGHFKPVAPNGKQPFVVVI
                     PPPNVTGVLHMGHGLNNSMQDILVRFHRMNGQPTLWLPGTDHAGIATQHVVEKKLRAQ
                     GIDRRELGREKFVEETWKVTNEHHDVIKKQLEKIGSSCDWGRERFTLDDGLSKAVREV
                     FVTLYERGLVYKGEYLVNWCPSCGTALSDDEVEHADEAGAMYHIRYEIVDPPAGGPTH
                     IEIATTRPETLLGDTAVAYHPDDERYQALAGAKLRLPLTDRVIPMVADAYVDREFGTG
                     LVKITPAHDPNDFEVGRRHDLPRVNILNPDGTLNDTCPEKYRGLKAKDARELVVADLE
                     ALGLFIKKEPITHAVGHCYRCHSVVEPFLSKQWFVRMKPLADKALAAWRAGDVRFYPQ
                     RWENTYENWLTNIRDWCISRQLWWGHRIPAWHCAHCGKDVVAREEPLECPFCKSTELA
                     QDEDVLDTWFSSWLWPFSTLGWPTETADLRKYYPTSTLLTGFDIIFFWVARMIMAGLE
                     FTGQVPFRDVCMTTLVRDKQGRKMSKSLGNGIDPLEIVDEYGADALRFTLAFLYTSTQ
                     DILINKDDFKLGSKFANKVWNASRYILMNLEGRELVAEPALNDADLWIRARLRSAASQ
                     IREALASYRFNDAAGAAYEYFWNDFCDWYVEATKLSTRSDSDAEKDRAVTVLLGVLEE
                     SLALLHPFLPFVTEEIYGKLPNARGRLIARPYPDGDPACAASDPELLARFAALKELVT
                     LVRSLRSEFGIAPEQRLRLALRFDDGFDGGEFIR"
     gene            2432..>2899
                     /locus_tag="PLQ29_09040"
     CDS             2432..>2899
                     /locus_tag="PLQ29_09040"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPG86829.1"
                     /translation="MNAIDQYARLVVRVGVNLRKGQTLVVSSPIECADFARSIQKEAY
                     EAGAREVVFRWNDEKSARVTYDLAPDEIFDESPEWLRVFSDGYADAGAAFVSIAASDP
                     ELMKGVDPKRISRKNKANNVALEHYRSRPMSNRNAWCVASVPTEAWARKVFPGK"
BASE COUNT          470 a          908 c          991 g          530 t
ORIGIN      
        1 gcgtatgaac tcgccgccgt cgaagccatc gtcgaagcgc agcgccagcc tgaggcgctg
       61 ctccggcgct atgccgaact cgctcctgag gctgcggacc agcgtgacca gctccttgag
      121 ggcggcgaag cgcgccagga gctcgggatc ggaggcggcg caggccgggt cgccgtcggg
      181 atagggcctg gctatcaggc gcccgcgagc gttcggtagc ttgccgtaga tctcctcggt
      241 gacgaagggc aggaaggggt gcaggagcgc cagcgattcc tcgaggacgc cgagcagcac
      301 agtcaccgcc cggtccttct cggcgtcgga atccgacctg gtggacagct tggtcgcctc
      361 gacgtaccag tcgcagaagt cgttccagaa atactcgtag gcggctccgg cggcgtcgtt
      421 gaagcggtac gaggcgaggg cctcgcgtat ctgcgacgcg gccgagcgca gcctggcgcg
      481 aatccacagg tccgcgtcgt tcagggccgg ctcggcgacc agctctcggc cctcgaggtt
      541 catcaggatg tagcgactgg cgttccagac cttgttggcg aacttgctgc cgagcttgaa
      601 gtcgtccttg ttgatcagga tgtcctgggt cgaggtatag aggaaggcca gcgtgaagcg
      661 cagggcgtcc gcgccgtact cgtcgacgat ctcgagcggg tcgatgccgt tgcccagcga
      721 cttactcatc ttgcggccct gcttatcgcg caccagcgtg gtcatgcaga cgtcgcggaa
      781 cggcacctgg ccggtgaact cgaggccggc catgatcatc ctggccaccc agaagaagat
      841 gatgtcgaag ccggtcagga gcgtcgaggt cgggtagtac ttcctgaggt ccgcggtctc
      901 ggtgggccag ccgagcgtac tgaagggcca gagccagcta ctgaaccagg tgtcgagcac
      961 gtcctcgtcc tgggccagct cggtcgactt gcagaagggg cactcgagcg gctcctcgcg
     1021 ggcgacgacg tccttgccgc agtgggcgca gtgccaggcc gggatgcgat ggccccacca
     1081 cagctggcgg ctgatgcacc agtcgcgtat gttcgtcagc caattctcgt aggtgttctc
     1141 ccagcgctgg gggtagaagc gcacgtctcc ggctcgccag gcggcgagcg ccttgtcggc
     1201 cagcggcttc atcctgacga accactgctt ggacaggaag ggctcgacga ccgagtggca
     1261 gcggtagcag tggccgaccg cgtgggtgat aggctccttc ttgatgaaca ggccgagggc
     1321 ctcgaggtcg gcgacgacca gctcgcgcgc gtccttggcc ttgagcccgc ggtacttctc
     1381 ggggcaggta tcgttgagcg tgccgtccgg gttgaggata ttgacgcgcg gcaggtcgtg
     1441 ccttctgccg acctcgaagt cgttcgggtc atgggccggc gtaatcttga cgaggccagt
     1501 gccgaactcg cggtcgacgt aggcgtcggc gaccatgggg atgacgcggt cggtcagcgg
     1561 cagcctgagc ttggcgccgg ccagcgcctg gtagcgctcg tcgtccgggt ggtaggcgac
     1621 ggccgtgtcg ccgagcaggg tctcgggccg cgtggtcgct atctctatgt gggtcgggcc
     1681 gccggccgga gggtcgacga tctcgtagcg tatgtggtac atggcgcccg cctcgtcggc
     1741 gtgctcgacc tcgtcatcgg acagcgcggt gccgcaggac gggcaccagt tgaccaggta
     1801 ctcgccctta tacacgaggc cgcgctcgta cagcgtcacg aacacctcgc gcacggcctt
     1861 ggacaggccg tcgtcgagcg tgaagcgctc gcgcccccag tcgcaggagg agcctatctt
     1921 ttctagttgc ttcttgatca cgtcgtggtg ctcgttcgtg accttccagg tctcctccac
     1981 gaacttctcg cggccgagct ctcggcggtc tatcccctgg gcgcgcagtt tcttctcgac
     2041 gacgtgctgg gtggcgatac cggcgtggtc ggtgcccggc agccagagcg tcggctggcc
     2101 gttcatgcgg tggaagcgca cgagtatgtc ctgcatcgag ttgttcaggc cgtggcccat
     2161 gtgcagtacg ccggttacgt tcggaggggg aatgacgacg acgaacggct gtttcccatt
     2221 gggcgcgacg ggcttgaaat gcccttcagc ctgccaggcc tcgtagacgc ggtcctcgaa
     2281 ttcctttggg tcgtaggcct tggccagttc gacggctttc atgggtaggt gcctccgatg
     2341 cttctcgtag cggatactgt accaaaaagc tggcggctgt gcaaaggccc gaatcgtggt
     2401 atcgtattcg cgcccgaata aaggagtctc aatgaatgcg atcgatcaat acgccaggct
     2461 cgtggtccga gtcggcgtca acctacggaa aggccagacc ctcgtcgtca gttcgcctat
     2521 cgaatgcgcc gacttcgccc gctcgatcca gaaggaagcc tacgaggccg gcgcccgcga
     2581 ggtggtcttc cgctggaacg acgagaagtc ggcccgcgtc acctacgacc tggccccgga
     2641 cgagatcttc gacgagtcgc ccgagtggct acgggtcttc tccgacggct acgccgacgc
     2701 cggggccgcc ttcgtcagca tcgccgcttc cgaccccgag ttgatgaagg gcgtcgaccc
     2761 caagcgtatc agccgcaaga acaaggccaa caacgtagcg ctcgagcact accgctcccg
     2821 gccgatgagc aaccgcaacg cttggtgcgt cgcgtcggtg ccgaccgagg cctgggcccg
     2881 caaggtcttc ccgggcaag
//
LOCUS       DAOPHV010000338         2898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_48054_length_2898_cov_3.692578, whole genome shotgun sequence.
ACCESSION   DAOPHV010000338 DAOPHV010000000
VERSION     DAOPHV010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48054_length_2898_cov_3.692578"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PLQ29_09045"
     CDS             complement(<1..751)
                     /locus_tag="PLQ29_09045"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPG86830.1"
                     /translation="MHAIRQRSEAAKVRRTFYSISLLIVIVVFIVFTTSHFTSLKRQN
                     DESFKRLEELMVTMTKVYLKDTVSRTLADIDLERKMIRLDLQVAAGDAEAESRADELV
                     KARSIVRIRETRLKEDGYLWASELLTPAGGRDYARRIAHGRYPDSEGTLLSTEASSGL
                     NASQYREELDGLLADGEVYYTYYTPKNGEPLPSRMLVYAALYPDFSWIVATGVYIDDI
                     ERTILLERKIAEADLEANAASSITTLVLALLA"
     gene            833..1327
                     /locus_tag="PLQ29_09050"
     CDS             833..1327
                     /locus_tag="PLQ29_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPG86831.1"
                     /translation="MVDTAALQAYSLFGGLSAEQIGLIRPLMGTAIFEAGEVIIREGQ
                     PNDSIYFITEGRVDVTRCGVSIVELPEGQTFGEMELLDVMPTAATVTAVMPVKVVTIS
                     NRCVREVYARDPKVFGIVMMNLARDLSRRLRRMDELACDASLPNHTAFLRVPAGDEPA
                     EYAR"
     gene            1338..>2898
                     /locus_tag="PLQ29_09055"
     CDS             1338..>2898
                     /locus_tag="PLQ29_09055"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPG86832.1"
                     /translation="MPDYSDFLKRQARDSLSTDALYAIHGETAFGNPAWASARSRILI
                     VRLSPFRDVERSTPHEFLFREARAAMPEAYMDFCFFPTRRDRAVLDAAGVPWMHGLAS
                     GRGAIEYDALLVSNSYTLELVNLAPFLEASGIPASRRERDAAVSAAAGGARFPLIALG
                     GSNAMASAAQYDEASGDAIVDAVFFGEGEGAVRELIPGLAAAALAGGSSRLAALETLA
                     RRVRGFWPTALRIEVDQAKAPADAYPQGAPPVLAGAEAGTSRLEITRGCPSFCSFCFE
                     GWERKPFRERPLASVVAEAKRLKAATGVDTVELASYNFNAHGEVVAVIESLHKIFWGV
                     GFQSQRVDILARSPSLVRFEVAAGKRSFTVGVEGVSSRMRAYYSKGLDEADLRSVLER
                     LVREGARELKLFYILSGYEADDDLAELSAFVGWLRGVVAAQAAPPRVMFSAGELVRMP
                     FTPLRYEALILDEAVYAAIRSRFEAIVESGGFEARAPERFDEYCLSQLLASAPDGSFA
                     LLLAMAERGYGY"
BASE COUNT          444 a          962 c          945 g          547 t
ORIGIN      
        1 ctgccagtag cgccagcacg agggtcgtaa tgctcgacgc ggcgttcgcc tcgaggtcgg
       61 cctccgctat cttccgctcg agcaggatgg tgcgctcgat gtcgtcgatg tagacgccgg
      121 tggccacgat ccaagagaaa tccggatata gggcggcgta gaccagcatc cgcgacggta
      181 gcggctcgcc gttttttggc gtgtagtagg tatagtagac ctctccgtcc gccaataggc
      241 cgtcgagttc ctcccggtat tgcgaggcgt tcaaacccga cgaagcttcg gtcgacagga
      301 gcgtaccctc ggaatcggga tagcgcccgt gtgcgatgcg cctggcgtag tcccggccgc
      361 cggccggggt gagcagctcg ctggcccaga ggtagccgtc ttctttaagg cgagtctccc
      421 tgatcctgac gatggagcga gccttgacca gttcgtcggc tctgctctcg gcctcggcgt
      481 ctccagcggc cacctgcaga tccagcctga tcatcttccg ctcgaggtct atatccgcca
      541 aggtgcggct cacggtatcc ttcaggtaca ccttggtcat ggtgaccatg agttcttcga
      601 gcctcttgaa gctctcgtcg ttctggcgct tgagcgaggt gaagtgggag gtagtgaata
      661 cgatgaagac gacgatgacg ataagcaggc ttatcgagta gaacgtacgg cgtaccttgg
      721 cggcttccga ccgctgcctt atggcgtgca tatgccgtct cccatgcatg caactatacg
      781 agataaaatg atcattgtca ctgtgctgct aatatggtac agtatacaag atatggtaga
      841 cacggcggcg ttacaggcct attcgttgtt cggcggcttg agcgctgagc agatcggcct
      901 catccggcct ttgatgggca ccgcgatctt cgaagccggc gaggtcatca tccgcgaagg
      961 ccaacccaac gattcgatct acttcatcac cgagggccgc gtggacgtca cgcgttgcgg
     1021 cgtgtccatc gtcgagctgc ccgagggcca gaccttcggc gagatggagc tgctcgacgt
     1081 aatgccgacg gccgccaccg tcacggccgt catgccggtc aaggtcgtca ccatatccaa
     1141 ccgttgcgtg cgagaggtct acgcccgcga cccgaaggtc ttcggcatcg tcatgatgaa
     1201 cctggcccgc gacctgtcga ggcgtctccg gcggatggac gagctggcct gcgacgcctc
     1261 gttgccgaac cacaccgctt tcctgcgggt tccggctggc gacgagccgg ccgaatacgc
     1321 ccgatagggt agacgcggtg cccgattact ccgatttcct caagcgccag gcgcgcgaca
     1381 gcctctcgac cgacgctctg tacgccatcc atggcgagac ggccttcggg aacccggctt
     1441 gggcctcggc ccgctcgcgc atccttatcg tccggctgtc gccattccgc gacgtcgagc
     1501 gctcgacgcc ccatgaattc ctgttccggg aggcgagggc cgccatgccc gaagcctata
     1561 tggacttctg cttcttcccg accaggcgcg accgggccgt cctcgacgcg gccggcgtgc
     1621 cgtggatgca cggcctggcc tcgggccgcg gggcgatcga gtacgacgcg ctgctcgtct
     1681 cgaattccta caccctggag ctcgtcaacc tcgcgccctt cctggaggcg tcgggcatcc
     1741 cggcgtcgag gcgagagcgg gatgccgccg tctcggcggc ggcaggcggc gctcgtttcc
     1801 cgctgatcgc gctcggcggc tcgaacgcga tggccagcgc cgcccagtac gacgaggcct
     1861 ccggcgacgc gatcgtcgac gccgtgttct tcggcgaggg cgagggcgcc gtccgcgagc
     1921 tgataccagg tctagcggcg gcggctctgg cgggcggctc gtccaggctc gcggcgcttg
     1981 agacgctcgc ccggcgggtt aggggcttct ggccgacggc cctgcgcatc gaggtggatc
     2041 aggccaaggc cccggcggac gcctaccccc agggagcgcc accggtccta gccggcgcgg
     2101 aggccggtac gagccggctc gagatcaccc gcggctgccc gagtttctgc tcgttctgct
     2161 tcgagggttg ggagcgcaag ccgttcaggg agcggccgct cgcctcagtc gtcgctgagg
     2221 ccaagcgcct caaggcggcc accggcgtcg atacggtcga gctggccagc tataacttca
     2281 acgcccacgg cgaggtcgtc gccgtcatcg agtcccttca caagattttc tggggcgtcg
     2341 gcttccagag ccagcgcgtc gacatcctgg cgcgctcgcc gtcactggta cgtttcgagg
     2401 tcgcggccgg taagcgatcg ttcacggtcg gcgtcgaggg cgtatcgtcg cgcatgcgcg
     2461 cctactactc caaggggctc gacgaggccg acctgcgatc ggtgctcgag cgcctggtcc
     2521 gcgagggcgc ccgcgagctc aagctcttct atatattatc aggctacgag gccgacgacg
     2581 acctggccga gctatcggcc ttcgtcgggt ggctacgtgg tgtcgtcgcg gcccaggccg
     2641 ccccgccgcg ggtcatgttc tccgctggcg agctcgtgcg catgccgttc acgccgctcc
     2701 ggtacgaagc gctgatcctc gatgaggccg tctacgccgc catccgctcc cgcttcgagg
     2761 ccatcgtcga atcgggcggc ttcgaggcgc gcgccccgga gcgtttcgac gagtactgcc
     2821 tgtcccagct cctcgcgtcg gcgccggacg gctccttcgc cctactcctg gcgatggccg
     2881 agcgcggcta cggctatg
//
LOCUS       DAOPHV010000339         2882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_48419_length_2882_cov_3.563141, whole genome shotgun sequence.
ACCESSION   DAOPHV010000339 DAOPHV010000000
VERSION     DAOPHV010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48419_length_2882_cov_3.563141"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="PLQ29_09060"
     CDS             complement(<1..1285)
                     /locus_tag="PLQ29_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HPG86833.1"
                     /translation="MNDILVHSEAVLGYGFVADKYLEDGESEYRLRNAQLDKPSDSYR
                     PLRHLDVEILIKNGNRCSDWGKLLVREPFDATLIRNSEFAGLVRIGSLERVVLEHHDM
                     MIPAGITNSRIISCDIGDQCAVHSCIYLAHYIVGDRCILMNNDDIHVSDHAKWGNGVI
                     KQGESEDVRIRLDLMNEAGGRSVLPFDGMLCADAYLWAKRRDDAALMERFEEMTDAAF
                     EHRRGEYGVIGSGSVLKSNRIIKDVRVGECAYIKGANKLKNLTINSSDAERTQIGEGV
                     ELVNGIVGYGCRVFYGCKAVRFVMGTNSALKYGARLIHSVLGDNSTVSCCEILNNLIF
                     PAHEQHHNTSFLIASLVRGQSNLAAGATIGSNHNSRANDGEIDAGRGFWPGLSTSVKH
                     SSRFASYCLLAKGAYRFELDVPFPFALVADDAARDR"
     gene            1463..2032
                     /locus_tag="PLQ29_09065"
     CDS             1463..2032
                     /locus_tag="PLQ29_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86834.1"
                     /translation="MGAPVYAVDDPADLDVEKAAFELFPLLVGTDNSVLRREYGSALA
                     DIIGGPGAFRKYVVGNTGDLALCRARLLERFRDNVRLLVAKTWVDGKDEVGKSEALAL
                     LDSFVGMVDAGDYPNAVPAFAGLARAVASLLFGDEADDPLFIDYVFRIDPRLGIFYWY
                     VDRLREQERADPELAMLELLIGVYALASF"
     gene            complement(2186..>2882)
                     /locus_tag="PLQ29_09070"
     CDS             complement(2186..>2882)
                     /locus_tag="PLQ29_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86835.1"
                     /translation="ILTSRPDQAVLRRLRSAIGELEEKSVPALSRTDVRAIVERRAGR
                     AVDASIVDRIAETTAGLPLLVRHAGSMLARATAPGEGDELSELPATLSAFGAESYRAI
                     RGPGDDADVRGRLFQLLGLAREPIGAAALHDLLQLDGEAVTLEQCREHLESMSSFLVE
                     ARVGLAAGWLEHGQVACVVELAPRHAYEGEAVRQAARAVEPEEGRRYAPGRQVAGAAD
                     HGHNRAARPIVMH"
BASE COUNT          468 a          960 c          973 g          481 t
ORIGIN      
        1 gcctgtcgcg ggccgcgtcg tcggccacca gcgcgaaggg gaacggcacg tccagctcga
       61 agcggtacgc gcccttggcc aagaggcagt acgaggcgaa gcgcgagcta tgcttgaccg
      121 aggtggacaa gcccggccag aagccgcgcc cggcgtcgat ctcgccgtcg ttggcccgcg
      181 agttgtggtt cgagcctatc gtcgcgccgg cggccaggtt cgactgcccc cggaccagcg
      241 aggcgatcag gaagctggtg ttgtggtgct gctcgtgggc cgggaagatc aggttgttga
      301 gtatctcgca gcacgagacc gtcgagttgt cgccgagcac cgagtgtatc agcctggccc
      361 cgtacttgag cgcggaattc gtgcccatca cgaagcgcac ggccttgcag ccgtagaaca
      421 cgcggcaacc gtagccgacg atgccgttga ccagctcgac gccctcgcct atctgggtac
      481 gctcggcgtc gctcgagttg atcgtcaggt tcttgagctt gttggcgccc ttgatgtagg
      541 cgcactcgcc tacccgcacg tccttgatga tgcggttgct cttgagcacg ctaccggagc
      601 cgatcacgcc atactcgcct cgccggtgct cgaaggcggc gtcggtcatc tcctcgaagc
      661 gctccatgag cgcggcgtcg tcgcggcgct tggcccagag gtaggcgtcg gcgcagagca
      721 taccgtcgaa gggcagcacg gaacggccgc cggcctcgtt catcaggtcg agccggatac
      781 gaacgtcctc gctctcgccc tgcttgatga cgccgttgcc ccatttggcg tggtcggaga
      841 cgtggatatc gtcgttgttc atgaggatgc agcggtcgcc gacgatatag tgcgccaggt
      901 agatgcacga gtgcacggcg cactggtcgc cgatgtcgca ggaaatgatg cgcgagttcg
      961 tgatacccgc agggatcatc atgtcgtggt gctcgagcac cacgcgctcg aggctaccga
     1021 tgcgaaccag gccggcgaac tcgctgttgc gtatcagggt ggcgtcgaac ggctcgcgca
     1081 cgagtagctt gccccagtcg gagcagcggt tgccgttctt aatgagtatc tcgacgtcga
     1141 gatggcgcag ggggcgatac gagtcggacg gtttgtcgag ctgggcgttc ctgagcctat
     1201 actcgctctc gccgtcctcg aggtacttgt cggcgacgaa cccatagccc aggacggcct
     1261 cggagtgtac aaggatgtcg ttcatggcgg ccttccagac gttcggtaat ggggagcgag
     1321 cctcccctcg accttaatcg taaccgctaa tgcctagctt ttcaacacac ggccgagccc
     1381 cgaggcagct cagcgccgtc gcgggggacc gaccgaaccg gtattattga caaagcggcg
     1441 gagggcgtga catggttgcc ccatgggcgc acccgtgtac gcggtcgacg atccggcgga
     1501 cctggacgtc gagaaggccg ccttcgaact cttccccctc ctcgtcggca cggacaactc
     1561 ggtgcttcgc cgcgagtatg gatcggccct ggccgacatc atcggcggcc cgggggcctt
     1621 caggaaatac gtagtcggca acacaggcga cctggccctc tgccgcgccc gcctcctgga
     1681 acgcttccgc gacaacgtac ggctcctcgt cgccaagacc tgggtggacg gcaaggacga
     1741 ggtcggcaag tccgaggccc tcgccctgct cgactccttc gtcggcatgg tggacgcagg
     1801 cgactacccg aacgccgtgc ccgccttcgc gggcctcgcc agggccgtcg cgtcgctgct
     1861 gttcggcgac gaggccgacg acccgctctt catcgactac gtcttccgca tcgacccgcg
     1921 gctcggcatc ttctattggt acgtcgaccg cctccgcgaa caggagcgag ccgacccgga
     1981 gctagccatg ctcgagctgc tgataggcgt ctacgcgctc gccagcttct agcccgggcc
     2041 acggcgcacg aaaacactca agatccgcga gagcgcgccg atactatcgt cgaggggcat
     2101 cgtatagctc ccgcgaccgc cttcggccgc gaggcgcggg cccgttctta tgccgggggg
     2161 ctaacagacc gtgtcgagaa tcggctcaat gcatgacgat cggacgcgcc gcacggttat
     2221 gaccgtgatc ggcggcgccg gcgacctggc gaccaggcgc atagcgccgg ccctcttcag
     2281 gctctaccgc tcgggccgcc tgccggacgg cttcgccctc gtaggcgtgg cgaggcgcga
     2341 gctcgacgac gcaggctacc tggccatgct cgagccagcc tgccgcgagg ccgacccggg
     2401 cttcgacgag gaaggagctc atcgactcga ggtgctcgcg gcactgctcc agcgtgaccg
     2461 cctcgccgtc gagctggaga aggtcgtgca gcgcggccgc gccgatcggc tcgcgcgcga
     2521 ggccgaggag ctgaaagaga cggccccgca cgtcggcgtc gtcacccggg ccgcggatcg
     2581 cgcggtagga ctcggcaccg aatgcggaca gggtggccgg tagttccgag agctcgtcgc
     2641 cttctccggg agcggtcgcg cgcgccagca tcgacccggc gtgccgcacg agaagcggca
     2701 accccgcggt cgtctcggcg atgcgatcga cgatggaagc gtcgaccgcg cggccggcgc
     2761 gtcgctcgac gatcgcgcgc acgtcggtgc ggctcagcgc aggcaccgac ttctcctcga
     2821 gctccccgat cgcggatcgg agccggcgca gcacggcctg gtcgggacgc gacgtcagga
     2881 tg
//
LOCUS       DAOPHV010000340         2872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_48620_length_2872_cov_4.669507, whole genome shotgun sequence.
ACCESSION   DAOPHV010000340 DAOPHV010000000
VERSION     DAOPHV010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48620_length_2872_cov_4.669507"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="PLQ29_09075"
     CDS             <1..1519
                     /locus_tag="PLQ29_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86836.1"
                     /translation="LVDREQRNKQLEEMEFSLSGLTDPSAQLEIGKLLSVRFLVSGSI
                     TDMGGPLLVSLSMLDVQTGAIVWNDQLTATSGKYAYIGAYFGKSLLRHFNATVSKETE
                     RALVASKDGDEASVIALSRGIEALDKGDTDEAKKQLTAARSIDPDNQVAAALLSKLAS
                     ASAKFKVVPERSVSYYNPAYLGGMEKDKAYFNLSFANLAWGLPNEDKGESQDYWLALD
                     ESGDYGTSERQMNLVAGYGFPLNGVLGLCAEVSGGGELSNAILRQYAPGDWMASGNDY
                     TYYISGGSITAGLRVSPWLSLGLGATVSYKKRDYYSEYLGSPPPELADPMNGYWLDTG
                     ISFGGMLAAALRSRSGGLSWDVSASWSNEVLHYFDTDPAALEFYEYGAPLYVEQTLSW
                     SPAGQKTFLALKQVNDLYFDRELYYSRLMPCVEQWFFGLFAVRLGVEGSIVYRDNLTE
                     LGWGATAGVTIPVWKLELDANYTLRQRPSRSLPGVTIPENVFFITVSADGLLFGD"
     gene            complement(1546..>2872)
                     /locus_tag="PLQ29_09080"
     CDS             complement(1546..>2872)
                     /locus_tag="PLQ29_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86837.1"
                     /translation="ADALEHVAEVPVALAHAALRPGGVAVFWSPERYGWFAQRLGAAF
                     AEVREVTAHDEVGRFRQELAQKTSQALDDFNRSYDGMAQDAARRARESVAAMGAAVED
                     YRREARLLQEGFDAAKAQMAGSLESERKARVKAFEEMDGQIKAFQAQTKLFERADELK
                     ASLGEAVEAMKADLARVESRRAEMAELETQYSRVKRLEDELGQKIAKFLAEKRRLDAM
                     EDDFKRLLALSQTVDQKLASVTSNNDQLTQLEAEFRRVVDAADEAAEKYERVEKKSNI
                     LNATADAIDKNFQAIGELERNVRTMDADIRELPDRVIDLKRSLDEVMSWKPKLDATVV
                     RLDEVDGVLADAEKRATELQKAREWLARAETRFDELNKKTQDHLKLLNDLLKDEPSAG
                     RKDKGAPPLSVQETVRKLSHQGWKVEEIARAVKLSRGEVELILELGGQD"
BASE COUNT          462 a          930 c          928 g          552 t
ORIGIN      
        1 gctcgtcgac cgggagcaac ggaacaagca gctcgaggag atggaattct ccctgtccgg
       61 cctgacggat ccctcggccc agctcgagat cggcaagctc ctttcggtac gtttcctggt
      121 gtccggatcg ataaccgaca tgggcgggcc gctactcgtc agcctttcaa tgctcgacgt
      181 ccagaccggc gcgatcgtgt ggaacgacca actgacggcc acgagcggca agtacgcgta
      241 catcggcgcc tacttcggca agtcgctgct ccgccatttc aatgcgacgg tctccaagga
      301 aacagagcgg gccctggtcg cgtccaagga cggcgacgag gcgtcggtga tcgccctgtc
      361 caggggcatc gaggcgttgg acaagggcga tacggacgag gcgaagaagc aactgaccgc
      421 cgcgcggagt atcgatccgg acaaccaggt cgcggctgcc ctgctatcga agctagcctc
      481 ggcgtcggcc aagttcaagg tcgtacctga gcgctccgtc agctactaca acccggctta
      541 tctcggaggc atggaaaagg ataaggcgta cttcaacctg tccttcgcca acctggcctg
      601 gggcttgccg aacgaggaca agggagagag ccaggattat tggctggccc tggacgagtc
      661 gggagactat ggaacctccg agcgccagat gaacctggtc gccggatacg gcttccccct
      721 caacggcgtt ctcggcctgt gcgcagaggt ctcgggcggg ggtgaactga gcaacgcgat
      781 acttaggcaa tacgctccgg gcgattggat ggcctcgggt aacgactata cctactacat
      841 atccggaggc tcgatcaccg ctgggctacg agtctcgcca tggctgagcc tcggactcgg
      901 cgctaccgta tcgtataaga agcgcgacta ttatagcgag tatctcggat cgccccctcc
      961 ggaactagcg gatccgatga atggatattg gttggatacg gggatctcgt tcggcgggat
     1021 gctcgcggcc gcgcttaggt cccgttcggg cgggctttca tgggatgtct ccgcgtcctg
     1081 gtcgaacgag gtgctgcact acttcgatac cgatcccgcg gcgctcgaat tttacgagta
     1141 cggagcgccg ctgtacgtgg aacagaccct ctcctggtca ccggccggcc agaagacctt
     1201 cctcgccttg aagcaggtca acgacctgta cttcgaccgg gaactctact atagccgcct
     1261 catgccgtgc gtcgagcagt ggttcttcgg ccttttcgcg gtacggctag gcgtcgaagg
     1321 ctcgatcgtg tatcgggaca atctgaccga gctcggctgg ggggccacgg ccggcgtgac
     1381 gataccggta tggaagctcg aactggacgc gaactacacc ctcaggcaac ggccctcgcg
     1441 atctctgccc ggagtcacca tccccgaaaa cgtcttcttc atcacggttt cggcggatgg
     1501 cctgctgttc ggcgattaaa cggagggcgg ggcgtggggg ccccgctagt cctgcccgcc
     1561 cagctccagg atcagctcga cctcgccgcg cgacagcttg acggcgcgag ctatctcctc
     1621 gaccttccag ccctggtggg acagcttgcg cacggtctcc tggaccgaca ggggcggcgc
     1681 gcccttgtcc ttgcgcccgg cgctcggctc gtccttgagc aggtcgttga gtagcttgag
     1741 gtggtcctgg gtcttcttgt tgagctcgtc gaagcgcgtc tcggcgcggg cgagccattc
     1801 gcgggccttc tggagctcgg tcgcccgctt ctcggcgtcg gccagcacgc cgtccacctc
     1861 gtcgagcctg accaccgtgg cgtccagctt gggcttccag gacattacct cgtcgaggga
     1921 tcgcttgagg tcgatgacgc ggtcgggtag ctcccggatg tcggcgtcca tcgtcctgac
     1981 gttgcgctcg agctcgccga tggcctgaaa attcttgtcg atagcgtccg cggtagcgtt
     2041 caagatgttc gacttcttct cgacgcgctc gtacttctcg gccgcctcgt cggccgcgtc
     2101 gaccacgcgg cggaactcgg cctcgagctg cgtgagctgg tcgttgttcg aggtgaccga
     2161 ggccagcttc tggtcgactg tctgcgacag ggccaggagg cgcttgaagt cgtcctccat
     2221 ggcgtcgagg cggcgcttct cggcgaggaa tttggctatc ttctgtccga gctcgtcctc
     2281 gagccgtttg acgcggctgt actgggtctc gagctcggcc atctcggccc ggcgcgactc
     2341 gacgcgggcc aggtcggcct tcatcgcctc gaccgcctcg ccgagcgacg ccttgagctc
     2401 gtcggcccgc tcgaacagct tcgtctgggc ctggaacgcc ttgatctgtc catccatctc
     2461 ctcgaaggcc ttcacgcggg ccttccgctc cgactcgagc gagccggcca tctgagcctt
     2521 ggcggcgtcg aagccttcct ggagcaggcg ggcttcgcgg cggtagtcct cgacggcggc
     2581 gcccatggcc gcgaccgatt ccctggccct gcgagcggcg tcctgcgcca tgccgtcgta
     2641 ggagcggttg aagtcgtcca gggcctggga cgtcttctgg gcgagctcct gtcggaagcg
     2701 gccgacctcg tcgtgagccg tgacctcgcg cacctccgcg aacgcggcgc cgagtcgttg
     2761 ggcgaaccac ccatagcgct ccggcgacca gaacacggcg accccgccgg gccgcagggc
     2821 cgcgtgcgcg agcgcgacgg gcacttccgc gacgtgttcg agagcatccg ca
//
LOCUS       DAOPHV010000341         2863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_48842_length_2863_cov_3.993234, whole genome shotgun sequence.
ACCESSION   DAOPHV010000341 DAOPHV010000000
VERSION     DAOPHV010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48842_length_2863_cov_3.993234"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLQ29_09085"
     CDS             <1..465
                     /locus_tag="PLQ29_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPG86838.1"
                     /translation="VPGASATQNSSSAAASIGPDTPIPLSTGALPLKVLDAVLKSLPV
                     DVSFVGADDAVMYYSDSPHRVFPRSPAIIGRAVQNCHPPKSVHIVEQIVEAFKRKEKD
                     KADFWIQMGDRFIVISYKPIYDADGTYLGTLEMSGDATDVRALEGQRRLLDW"
     gene            609..1802
                     /locus_tag="PLQ29_09090"
     CDS             609..1802
                     /locus_tag="PLQ29_09090"
                     /inference="COORDINATES: protein motif:HMM:NF008887.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HPG86839.1"
                     /translation="MKKALNGTVSWVGKIDWELTRFHGDEYSTHKGSSYNSYVIEEGK
                     TVLIDTVWNPYAKEFVDNLEAEGLLDKLDAVIAQHAESDHSGAMPELMRRRPDLPVYC
                     TANGVKSLKGLYHQDWDFRVVKTGDEYDLGNGKKLVFIEAPMLHWPDSMFSYMTGDAI
                     LFSNDAFGQHLATERLYNDLVDQDVLWSECVKYYANILTPFSSLVKAKIDQVLSLGLP
                     VAMIATSHGVIWRDDPIQIVRKYLEWSQDYKEDRVTILYDTMWQGTRVMADTLAKAIA
                     EASPSTTVVSHCLSKFDKNDAVTDVFRSKAVLVGSPTVGKGILTAVAGVLEEIKGLRF
                     KGKKAGAFGAYGWSGESVKVITDLLKASGFDVVDDGIKALWNPDDAAIAATAAYGKAF
                     AAKLG"
     gene            1816..2151
                     /locus_tag="PLQ29_09095"
     CDS             1816..2151
                     /locus_tag="PLQ29_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPG86840.1"
                     /translation="MLEKEYRFTETDAFTMEKIIDDERVNINHIVVEPGGAVPVHASN
                     SFVHQIVVRGTLSLSLEDGPEEAHPAGSIVAVPFNLKMAIRNRGDETLEFFVVKAPNP
                     RDMPPTKAI"
     gene            2219..>2863
                     /locus_tag="PLQ29_09100"
     CDS             2219..>2863
                     /locus_tag="PLQ29_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPG86841.1"
                     /translation="MSKHTYRGAERRPAAGIVLAAVLTAAIAVSLSSACATGRADGSG
                     GEAIAPLAPAVRPAEYGPRPLCVGHRGAAGLAPQNTMASFRAGIAAGVDAIELDIHLS
                     SDGRLVVIHDPVVDKLTDGKGRVSAMSLAELRALDASARFPGWTGEPQRLPTLEEALD
                     LLAEPGNEKVGLQLEIKLDEDGSRHPGIEAAALAAVRDRGLIDRTIVISFDFPTL"
BASE COUNT          558 a          971 c          886 g          448 t
ORIGIN      
        1 gttccgggcg cctcggcgac tcaaaatagc tcgagcgccg cggcgagcat aggccccgac
       61 acgccgatac cgctgtccac cggcgccctg ccgctcaagg tgctcgacgc cgtgctcaag
      121 agcctgccgg tcgacgtgtc cttcgtcggc gcggacgacg ccgtgatgta ctactccgac
      181 tcgcctcacc gcgtgttccc gcgcagcccc gccatcatcg gccgcgccgt gcagaactgc
      241 catccgccca agagcgtcca catcgtcgag cagatagtcg aggcgttcaa gagaaaggag
      301 aaggacaagg ccgatttctg gatccagatg ggcgaccgct tcatcgtcat ctcgtacaag
      361 cctatctacg atgccgacgg gacctacctg ggcacgctcg agatgagcgg cgacgcgacc
      421 gacgttcggg cgctcgaggg tcagcgcagg ctgctcgact ggtaacggag tcccatgcga
      481 tatcatcgtc gctaaaacta cttgctccat ttaccgtatc ctggtattat aatacctgaa
      541 tacggtaaac ggagattcgg cttcactcgg gagagcggaa acgcgcaacg cgcgaaagga
      601 gaggcggcat gaagaaggct ttgaacggaa cggtgtcctg ggtcggcaag atcgactggg
      661 aactgacgcg attccatggc gacgagtact cgacccacaa gggctcgagc tacaacagct
      721 acgtcataga ggagggcaag accgtgctga tcgacacggt atggaacccc tacgccaagg
      781 aattcgtcga caacctcgag gctgagggcc tcctcgacaa gctcgacgcc gtcatagcgc
      841 agcacgccga gagcgaccac tcaggcgcga tgccggagct catgcgccgc cgtcccgacc
      901 tgcccgtcta ctgcacggcc aacggcgtca agtctctcaa agggctctac caccaggact
      961 gggacttccg cgtcgtcaag accggcgacg agtacgacct gggcaacggg aagaagcttg
     1021 tcttcatcga ggcgccgatg ctccattggc cggacagcat gttctcgtac atgaccggcg
     1081 acgccatcct gttctccaac gacgccttcg gccagcacct ggccaccgag cgcctctaca
     1141 acgacctcgt agaccaggac gtgctctggt cggagtgcgt caagtactac gccaacatac
     1201 tcacgccctt ctcgtcgctc gttaaggcca agatcgacca ggtgctgtcg ctcgggctgc
     1261 cggtcgccat gatcgccacg agccacggcg tcatctggcg cgacgacccg atccagatcg
     1321 tgcggaagta cctcgaatgg tcgcaggact ataaggagga cagggtcacc atactctacg
     1381 acacgatgtg gcagggcacg cgggtcatgg ccgacacgct ggccaaggcc atcgccgagg
     1441 cgtcgccgtc caccacggta gtatcgcact gcctgtccaa gttcgacaag aacgacgccg
     1501 tcaccgacgt tttccgctcc aaggccgtgc tcgtcggctc gcccaccgtg ggcaagggaa
     1561 tcctgaccgc cgtcgcgggc gtgctcgagg aaataaaggg cctgcgcttc aagggcaaga
     1621 aggccggcgc cttcggcgcc tacggctggt cgggcgagtc ggtcaaggtg atcaccgacc
     1681 tgctcaaggc ctccggcttc gacgtggtcg acgacggcat caaggcgcta tggaacccgg
     1741 acgacgcggc tatagccgcc acggcggcct acggcaaggc tttcgccgct aagctgggct
     1801 gatcggagta gaggaatgct ggagaaagaa taccgtttta ccgaaacaga cgccttcacg
     1861 atggagaaga taatcgacga cgagcgcgtc aacatcaacc acatcgtcgt ggagccgggc
     1921 ggagcggtgc cggtgcacgc gtcgaactcg ttcgtacacc agatcgtcgt gcgcgggacc
     1981 ttaagcctgt ccctcgagga cggccccgag gaggcccacc cggccggcag catcgtagcg
     2041 gtgccgttca acctcaagat ggccatacgg aaccgcggcg acgagaccct cgagttcttc
     2101 gtcgtcaagg cgccgaaccc gcgggacatg ccgccgacca aggcgatcta agacatagtc
     2161 atgtcgcggc cgcggcgtcg acgctgcggc cgcgacgctt cggtgctata ctggctccat
     2221 gagcaagcac acgtatcgcg gcgccgagcg ccgtcccgcc gccgggatcg tcctggccgc
     2281 cgtcctgacc gccgccatcg ccgtatcgct gtcgtcagcc tgcgccacgg gccgggccga
     2341 cggctccgga ggggaagcca tagctcccct ggctccggcc gtcaggcccg ccgagtacgg
     2401 cccgcgccca ctctgcgtcg gccaccgcgg cgcggccgga ctcgcgccgc agaataccat
     2461 ggcgtcgttc cgcgccggca tcgcggccgg cgtcgacgcc atagagctgg atatacacct
     2521 aagctccgac ggccgcctcg tcgtcataca cgacccggtc gtcgacaagc tgacggacgg
     2581 caagggccgc gtatcggcta tgagcctcgc cgagctccgc gccctcgacg cgagcgcgcg
     2641 cttcccgggc tggacggggg agcctcagcg cctcccgacc ctcgaggaag cgctcgacct
     2701 gctcgcggag cccggcaacg agaaggtcgg cctccagctc gagatcaagc tcgacgagga
     2761 cggctcgcgc cacccgggta tcgaggccgc cgcactggcc gccgtccgcg atcgcggcct
     2821 tatcgaccgc acgatagtca tatcgttcga cttcccgacg ctg
//
LOCUS       DAOPHV010000342         2855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_49036_length_2855_cov_3.616786, whole genome shotgun sequence.
ACCESSION   DAOPHV010000342 DAOPHV010000000
VERSION     DAOPHV010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49036_length_2855_cov_3.616786"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="PLQ29_09105"
     CDS             complement(<1..834)
                     /locus_tag="PLQ29_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86842.1"
                     /translation="MIASSCKYESLSSRETFNASVRHVKTTVALAERIAGGENLADAL
                     DALIADKTVSGDEILPFVSMLLIDKFGYRAAAKNAQSTVSGFEAALKATASWNAFDLV
                     MLYNHPDVGVIAVNPKNPAHAAKIDRVNKSELLVAYVGRFGKDYDASLAEKAADALLA
                     LYGGESPKEIPALLKGPCVYKAPKEAPAPKPTRAPKVSKGAKPQKSKDGATAKAAAPV
                     AERLPAATQKPAPGPKAGSRMTPMYGVLVTNELFHNGNVEAWKRIIDSFKVKHPDLDV
                     LV"
     gene            complement(898..>2855)
                     /gene="cfpA"
                     /locus_tag="PLQ29_09110"
     CDS             complement(898..>2855)
                     /gene="cfpA"
                     /locus_tag="PLQ29_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytoplasmic filament protein CfpA"
                     /protein_id="HPG86843.1"
                     /translation="QKEVDQLLHEETNKVLHHINSRLPKEVLERLDVMGGLKEKVYNY
                     FNQNYQNMFNRYIVTTEDEMVKKVRNFIDKEEMKSLARYTPKEIATLLDEVGGADKFN
                     TGETEKSMVNMFGHLQGHIQRGVNELENLTNSLLRQKTDVGAFIRGENAYSVVKCAFK
                     DNTYKPKTVTDVKLSVNILDSELISPIFHYQVTVEYLIKDLIAKHIIDTIDKDIEALK
                     DQLVDEGKEELNDTEIIFERMKKVKDHTDDEVENVKSKRYSIVAKDLMDRINNLRAEI
                     DPSTFDQLNIRENLKKIIDIENIRNRGFNTAVNSITSILDTSKMGYQYIENLKNARLL
                     YIREYEDTDTAHLPDERYQIRLQYYDNAQLIEERKAYDVQITSFETEVQHLWDVLEMM
                     YEDSKFLKRITDFDDLAKLMKSKIRKRIKEKSGEPLYEDIAKVWDEIAFVKPAETDVE
                     SLNCTYIYEKDKVKNRLILMRDKLKNMYAYQYPIQRRVVEDRLTFLEKEFQRFDYMIN
                     PYHIQPGLLLDVDICSIKRKKATLDGMANVLNEFLHGVSKGFQDAAFASFSRRRSTVR
                     EDIAQSFAPAQIEDAPESASAGYLAMLNAEPESPALPEATMATAAKKRGGRPKGSGVV
                     DATPKKAGAKRGPKPRSKKDEGGIREL"
BASE COUNT          457 a          813 c          893 g          692 t
ORIGIN      
        1 gacgagcacg tcgaggtccg ggtgcttgac cttgaagctg tcgatgatgc gcttccaggc
       61 ctcgacgttg ccgttgtgga acagttcgtt cgtgacgagc acgccgtaca tcggggtcat
      121 cctggagccg gccttcggcc cgggcgcagg cttctgggtc gcggcgggaa gccgttccgc
      181 tacgggagcc gccgcctttg ccgtggctcc gtccttggac ttctggggct tagcgccctt
      241 cgataccttg ggagccctcg tcggcttcgg ggccggcgct tccttcgggg ccttgtacac
      301 gcatggtccc ttaagcaggg cgggaatctc cttaggagac tcgccgccgt acagggccag
      361 gagcgcgtca gcagccttct cagcgagcga ggcgtcatag tccttgccga agcggccgac
      421 gtaggcaacg agcagctccg acttattgac gcggtctatc ttggcggcgt gggccgggtt
      481 cttgggattg accgctatga caccgacatc cgggtggtta tagagcatca ccaagtcgaa
      541 cgcgttccag gaggccgtgg ccttgagcgc cgcttcgaaa ccggacacgg tggattgggc
      601 gttcttcgcg gccgctcggt acccgaactt atcgatgagc aacatcgaga cgaacggcag
      661 tatctcgtcg ccgcttacgg tcttgtcggc gatcaaggcg tcaagcgcat ccgccaggtt
      721 ctcgcccccg gcgatcctct cggccaaggc caccgtcgtc ttgacatgac gaaccgacgc
      781 gttgaacgtt tccctggatg acaaggactc gtacttgcag ctcgaggcaa tcatgctttc
      841 cccttcctta atgaaaagcg ccccccgaag accggggggc gcgttcgcta cgctcgctta
      901 gagttcgcgg atgccgccct catccttctt ggatcgcggc ttgggtcccc gcttggcgcc
      961 agccttcttg ggggtagcgt cgacgacccc gcttccctta ggccgcccgc cgcgcttctt
     1021 ggctgcggta gccatggtcg cctccggcag ggccggggac tcgggctcgg cattgagcat
     1081 ggccaggtag ccagccgaag ccgactcggg agcgtcctcg atctgcgccg gggcgaagct
     1141 ctgggcgatg tcctcgcgga cggtggatcg cctgcggctg aacgaggcga acgccgcgtc
     1201 ctggaagccc ttggacacgc cgtgcaggaa ctcgttgagc acgttggcca tgccgtcgag
     1261 cgtggccttc ttgcgcttga tcgagcagat atccacgtcc aggaggaggc ccggttggat
     1321 gtggtacggg ttgatcatgt agtcgaagcg ctggaattcc ttctcgagga aggtcagcct
     1381 gtcctcgacg acgcggcgct ggatcgggta ctggtaggcg tacatgttct tgagcttgtc
     1441 gcgcatcagg ataaggcggt tcttgacctt gtccttctcg tatatgtagg tgcagttcag
     1501 gctctcgacg tcggtctcgg caggcttgac gaaggcgatc tcgtcccaaa ccttggcgat
     1561 gtcttcgtag aggggctcgc cagacttctc cttgatccgc ttacggattt tgctcttcat
     1621 gagcttggcc agatcgtcga aatcggtgat gcgcttgagg aacttggagt cctcgtacat
     1681 catctcgagc acgtcccaga ggtgctgcac ctcggtctcg aagctggtga tctgtacgtc
     1741 gtaggccttg cgctcctcga tcagctgggc gttatcgtag tactggagtc ggatctggta
     1801 gcgctcgtcc gggaggtggg cggtatcggt atcctcgtac tcgcggatat agaggaggcg
     1861 ggcgttcttg aggttctcga tgtactggta gcccatcttc gaggtgtcga gaatcgaggt
     1921 gatcgagttg acggcggtgt tgaagccgcg gttgcggatg ttctcgatat cgatgatctt
     1981 cttgaggttc tcgcggatgt tgagctggtc gaaggtggac gggtcgatct cggcgcggag
     2041 attatttatc ctgtccatca ggtccttggc cactatcgag tagcgcttcg acttgacgtt
     2101 ctcgacctcg tcgtcggtgt gatccttaac cttcttcatg cgctcgaaga tgatctcggt
     2161 atcgttcagt tcttccttgc cctcgtcgac cagctggtcc ttgagcgcct cgatgtcctt
     2221 gtcgatcgtg tctatgatgt gcttggcgat caagtccttg atcaggtact cgacggtgac
     2281 ctgataatgg aagatcgggc tgatcagctc ggagtcgagg atgttgaccg acagcttgac
     2341 gtcggtgacg gtcttgggct tgtaggtgtt atccttaaag gcgcacttga ccaccgagta
     2401 ggcgttctcg ccgcggatga acgcaccgac atcggtcttc tgcctgagaa gcgagttggt
     2461 caggttctcc agctcgttga cgccgcgctg aatatgcccc tggagatggc cgaacatatt
     2521 gaccatcgac ttctcggtct cgccggtgtt gaacttgtcg gcgccgccaa cctcgtcgag
     2581 cagggtcgct atttcctttg gggtatagcg cgccagggac ttcatctctt ccttgtcgat
     2641 gaagttcctg accttcttca ccatctcgtc ctcggtcgtg acgatatagc gattgaacat
     2701 gttctggtag ttctggttga agtagttgta caccttctcc ttgaggccgc ccatcacgtc
     2761 gaggcgctcg agcacctcct tggggagcct ggagttgatg tggtggagaa ccttattcgt
     2821 ctcctcgtgc aggagctgat cgacctcttt ctgct
//
LOCUS       DAOPHV010000343         2855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_49039_length_2855_cov_3.537143, whole genome shotgun sequence.
ACCESSION   DAOPHV010000343 DAOPHV010000000
VERSION     DAOPHV010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49039_length_2855_cov_3.537143"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLQ29_09115"
     CDS             complement(<1..414)
                     /locus_tag="PLQ29_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPG86844.1"
                     /translation="MYDFIRCEVPDCRATALTGYSFCADHCHDPSAVAASMAALIRSS
                     SSVRSVNASGLRFEGLDLSGKSFYACSFSGTVLKNVAFTGCTFRMCFFDFCSADSCDF
                     SGIDAQFCSFAGARFLNISFERSELVHNNFDGVRTN"
     gene            557..838
                     /locus_tag="PLQ29_09120"
     CDS             557..838
                     /locus_tag="PLQ29_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86845.1"
                     /translation="MCSYCGSQLPQGTIGFRAVCADCGRELHICRHCGFYKPGAYRDC
                     SETVPEAVQDKERMNFCEFFKPNPASVKDGKAAKASASAKDAFDGLFGR"
     gene            857..1720
                     /gene="murI"
                     /locus_tag="PLQ29_09125"
     CDS             857..1720
                     /gene="murI"
                     /locus_tag="PLQ29_09125"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPG86846.1"
                     /translation="MHDLDSPIAFFDSGVGGLPYLEAARRLLPAERFVYLADRAGFPY
                     GTKSPERVRDLAVGAIRALVAKTAPKAVVIACNTATELAVDAIRADTPGLIVVGTVPA
                     IKPAAALSRVRRIGVVATEAAVAADYLTGLASAWAADCELVRIGDAGLVDFVERRLLG
                     STRDERLRAVAPSVRAMLERGVDVIVLGCTHFLHLSEEFGAVAGPGVSIVDSRDGVAA
                     RLASRLDEEGLRGSVGARRTGGCPDRRDAESDRPGEPSGRLFLTGAGDPGPVYGGFAE
                     RFGLSWAGCLP"
     gene            1717..2691
                     /gene="rsgA"
                     /locus_tag="PLQ29_09130"
     CDS             1717..2691
                     /gene="rsgA"
                     /locus_tag="PLQ29_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPG86847.1"
                     /translation="MSLGTVLSGANNSFSVLAEDGSVHYCALKGKKLKGAEGFYNPLA
                     AGDVVEWEPASAGTGMILSLRPRKSLFWRYNEKGKAIQAIAANMDLVVCVTSAGLPPF
                     RPRFIDRVSLEPASEGIPLIIALNKSDLGIDDDMAERMADYRRIGFETMELSAGTGAG
                     VDALRERVAGRTSVFVGQSGVGKSSLLNAIESGLGLKVGEVCEKYERGRHTTVAAILS
                     VLSDGVTRVIDTPGFRRLAVRGIDPVTLGACFPEIRAASAGCTLGSRCKHLDEPGCAV
                     VAALESGGVHEDRYESYARILAELEDTRAYAKKAGRPRRHHEDDYEDA"
BASE COUNT          510 a          930 c          935 g          480 t
ORIGIN      
        1 gttggtcctg acgccgtcga agttgttatg cacgagttcg gaacgctcga acgagatatt
       61 caggaacctg gcgccggcga aggagcagaa ctgggcgtct atccccgaga agtcgcagga
      121 gtcggccgag cagaagtcga agaagcacat cctgaaggtg cagccggtga atgctacgtt
      181 cttgagtacg gtcccggaga acgagcatgc gtagaacgac ttgcccgata ggtccagccc
      241 ctcgaaccgg aggccggagg cattgacgct cctgaccgac gaggaggaac ggatcagggc
      301 cgccatcgag gcggcgacgg ccgacgggtc atggcaatga tcggcgcaga aagagtaacc
      361 cgtcagggcc gtggcgcggc aatcgggaac ctcgcatcgt atgaaatcgt acatcggcag
      421 ggcctcctcg atagccgctc gcagggcgct aagccctgcg agcgaggata gcctaaaacg
      481 agactgcgct caagccgcgt tccatataat cgatccgtaa cttgaataat cctcgtctcg
      541 ggcgtacact gcgctcatgt gctcgtattg cggctcccag ctaccccaag gaaccattgg
      601 cttccgcgcc gtctgcgccg actgcggccg cgagttgcat atctgcaggc attgcggttt
      661 ctataaaccc ggtgcgtatc gtgattgctc cgagacggtc cccgaggccg tgcaggacaa
      721 ggagcggatg aacttctgcg agttttttaa accgaatcca gcctcggtca aagacgggaa
      781 ggccgccaag gcttcggctt ccgccaagga tgccttcgac ggcctgttcg gccgctgacg
      841 cgatcaggat tacccgatgc acgatctcga tagccctata gccttcttcg attccggagt
      901 cggcggcctg ccatacctcg aggccgccag gcgcctcctc ccggctgaac gcttcgtgta
      961 cctcgctgat agggccggct tcccctacgg tacgaaatcc ccggaacgag tcagggacct
     1021 ggccgtcgga gccatccgag ctctcgttgc caagaccgcg ccgaaagccg tcgtcatcgc
     1081 ctgcaatacc gcaaccgaac tggccgtcga cgctatccga gcagataccc cagggctgat
     1141 cgtcgtcggc acggtacccg cgatcaagcc ggccgccgcg ctgtcgcggg tccgcaggat
     1201 tggcgtcgtg gccactgagg ccgccgtcgc ggccgactac ctgaccggcc tggcctcggc
     1261 ctgggcggcc gactgcgagc tggtcaggat cggtgacgcc ggactggtcg acttcgtcga
     1321 acgccgcctg ctcggctcga cccgcgacga gcgcctgcgg gccgtcgcgc ccagtgtgcg
     1381 tgccatgctc gagcgcggcg tcgacgtcat cgtcctcggc tgtacgcatt ttctgcacct
     1441 gtccgaggag tttggcgccg tcgccggccc cggcgtgtcc atcgtcgact cgagagacgg
     1501 cgtggccgcc aggctggcgt cgcggctcga cgaggaaggc ctgcgcggga gcgtcggcgc
     1561 gcggcgaacc ggtggatgcc ccgacaggcg agacgccgag agcgaccgcc ccggagagcc
     1621 ctccggccgc ctcttcctca caggcgcggg cgaccccgga ccggtctacg gcggcttcgc
     1681 cgagcgcttc ggcctgtcgt gggcggggtg cctaccttga gcctcggtac ggtactgtcc
     1741 ggcgccaata actcgttctc cgtcctggcc gaggacggtt ctgtccatta ctgtgcgctc
     1801 aagggcaaga agctcaaggg tgcggaaggc ttctataacc cgctcgccgc cggagacgtg
     1861 gtcgaatggg agccggcctc ggcgggcacc ggcatgatcc tatcgctccg tccgcgcaag
     1921 agcctcttct ggcgctacaa cgagaagggc aaggccatac aggcgatagc cgccaacatg
     1981 gacctggttg tatgcgtcac gagcgcgggc ttgccgccgt tcaggcccag attcatagac
     2041 cgcgtatcgc ttgaacccgc ctccgaaggc atcccgctga tcatagcgct caacaaatcg
     2101 gatctgggaa tcgacgacga tatggccgag cgcatggccg attatcggcg catcggcttc
     2161 gagacgatgg agctgagcgc gggaacgggc gctggggtcg acgccttgcg ggagcgcgtg
     2221 gccggccgaa cgagcgtgtt cgtcggacag tccggcgtcg gcaagagctc cctcttaaac
     2281 gccatcgaat cgggcctcgg gctcaaggtc ggcgaggtct gcgagaaata cgagcgcggt
     2341 cggcatacga ccgtcgccgc gatactatcg gtgctatccg acggcgtcac ccgcgtcatc
     2401 gacacgcccg gtttcaggcg cctggcggtc aggggcatag accccgtgac cctcggcgcc
     2461 tgcttcccgg agatacgagc cgcctccgcg ggctgcaccc tcggtagccg atgcaagcac
     2521 ctcgatgagc ccggttgcgc ggtcgtggcc gccctcgagt cgggcggcgt ccacgaggac
     2581 cgctacgaga gctacgcgcg catcctggcc gagctcgaag atacgcgtgc ttatgccaag
     2641 aaagccggca ggccgcgacg acaccatgag gacgattacg aagatgcatg accacgcgct
     2701 atcgctactc gatttctacc gtatccgcga cgacgtcgcc ggatattgcc tgagcgagga
     2761 aggcgctgcc gccgtcaggg cgggcttgcc ctcggccgac ccggacgagg tggtggacgc
     2821 taaggctcgg gcctcggcgg tccgagagct gctcc
//
LOCUS       DAOPHV010000344         2848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_49202_length_2848_cov_3.444683, whole genome shotgun sequence.
ACCESSION   DAOPHV010000344 DAOPHV010000000
VERSION     DAOPHV010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49202_length_2848_cov_3.444683"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PLQ29_09135"
     CDS             complement(<1..597)
                     /locus_tag="PLQ29_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HPG86848.1"
                     /translation="MIRGWYTGASGMAAQQVRLDAIANNLANIDTDGYKRDVTVSKAF
                     AQLLLRRMGDDGQYLHPLGSGDMAPVIGKVGTGVEANEVFTELEQGALKGTDSDYDVA
                     LDGAGFMCVSTPQGERYTRNGSFVLGKEGYLETKEGYPVLGEAGPIRVKQNNFQIDKD
                     GRVWVNADYQDDPLDLVGREQNEWREPVLLDTLKLVDFE"
     gene            complement(656..1099)
                     /locus_tag="PLQ29_09140"
     CDS             complement(656..1099)
                     /locus_tag="PLQ29_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86849.1"
                     /translation="MAYDVSKSVKGDDAFYTVRWSPIVQADKYRIIGSVPAMGGIAEL
                     YYKDAGGKLNLYLLARSYYGGLRATLRAATDPETEKDERRRAVLVDHEGEIYYRYALV
                     ESQDDMSDVMFFFMSTYAPKIRFDHSGRYERIFLTEVDAGGLVTV"
     gene            1278..1592
                     /locus_tag="PLQ29_09145"
     CDS             1278..1592
                     /locus_tag="PLQ29_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86850.1"
                     /translation="MREIVERVLGAEDEAKSRIEAARKRASAVKADADEAAAAFVAKA
                     REKAVADTRARLDQARASAERLIVAAREAADAEAERNAGTVASERESLVAEIVGVIAG
                     RP"
     gene            1600..2682
                     /locus_tag="PLQ29_09150"
     CDS             1600..2682
                     /locus_tag="PLQ29_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HPG86851.1"
                     /translation="MRPVPSAIRTYGFLSAKLKARISKTMPPETMDRLIKARSLPEAV
                     QFLRGTAYQAIEEAYTATGDLRAGEALLHERELRLYTGLFRYLDEPVLSFVRALAARY
                     EIDQVKNAVRLWFDAHVRGRDVAGKSGYLFRGTIVNRMDVDAIVRAADIEGVAAALSG
                     TPYAAIARAELPAAQASRSVFSFELALDRRYYDEAFAAAASLGGRDREAAERALGVDV
                     DLQNVSWLIRFKDFYGMGAEDAMSRLIPRGRAIKPAAVAAVWDSERGSRGAGGIGLDL
                     LGRSYPGVGAMMGGGGSAAKLAVLEAAIRRVADDEAGKSLCGYPFSIGVVLAYFTKKR
                     AEMRAVMTVLNAKYYALAEERIRSSL"
BASE COUNT          467 a          898 c          984 g          499 t
ORIGIN      
        1 ctcgaagtcg acgagcttga gcgtatcgag gagcaccggc tcccgccact cgttctgctc
       61 gcggccgacg aggtcgagcg ggtcgtcctg gtagtcggcg ttgacccaga cgcggccgtc
      121 cttgtctatc tggaagttgt tctgcttgac gcgtatgggg ccagcctcgc cgagcaccgg
      181 gtagccttcc ttggtctcga ggtagccttc cttgccgagt acgaaggagc cgttgcgggt
      241 atagcgctcg ccctgggggg tcgagacgca catgaagccc gcgccgtcga gcgccacgtc
      301 gtagtcgctg tcggtgccct tgagggcgcc ctgctccagc tcggtgaaca cctcgttggc
      361 ctcgacgccg gtgccgacct tgccgatgac cggggccatg tcgccggagc cgagcgggtg
      421 caggtactgg ccgtcgtcgc ccatgcgcct gaggaggagc tgcgcgaagg ccttgctgac
      481 cgtcacgtcg cgcttgtagc cgtcggtgtc gatattggcg aggttgttgg ctatcgcgtc
      541 gagcctgacc tgctgggcgg ccatgccgct ggctcccgta taccatcctc tgatcatgcg
      601 cgtccctccg ccttctatta ctatatcggc ggcggaggcg cccgggttta gggacttaga
      661 ccgtcacgag gcccccggcg tcgacctcgg tcaggaagat gcgctcgtag cgccccgaat
      721 ggtcgaagcg gatcttcggg gcgtaggtgc tcatgaagaa gaacatgacg tcgctcatgt
      781 cgtcctgcga ttcgacgagg gcgtagcggt agtatatctc gccctcgtgg tcgacgagga
      841 ccgctcgcct gcgctcgtcc ttctcggtct ccgggtccgt agccgcgcgc agggtggcgc
      901 gcaggccgcc gtagtacgag cgcgcgagca ggtagaggtt gagcttgccg ccggcgtcct
      961 tgtagtacag ctccgcgatg ccgcccatgg ccggaaccga gcctatgatg cggtacttat
     1021 cggcctgtac gatgggagac cagcggaccg tgtagaacgc atcgtcgccc ttgacggact
     1081 tgcttacgtc gtatgccatc gcgggcatgg tagcacggta tgggcgcctg gtgatactcc
     1141 agggcggaat gccccccgca ctgtacattc tttgtaaaaa ggtataaact tcttcagtat
     1201 ccgctccatg gtcgacccca cccgccgacc ttagctccag aggagcggcg ctacgttcta
     1261 taaggaagta tcggatcatg cgtgagatcg tcgagcgtgt cttaggggcg gaggacgagg
     1321 ccaagtccag gatagaagcg gccaggaagc gagcgtcggc cgtcaaggcc gacgccgatg
     1381 aagcggccgc cgcgttcgtc gccaaggcgc gggagaaggc ggtggcggac accagggcca
     1441 ggctggacca ggccagggcc tcggccgaac gcctgatagt cgcggcccgc gaggcagccg
     1501 acgccgaggc cgagcggaac gccggtacgg tagcgtcgga gcgcgagtcg ctcgtcgcgg
     1561 agatcgtcgg cgtcatcgcc gggcggccgt gatggaggcg tgagacccgt gccctccgcg
     1621 atacggacct atgggttcct gagcgccaag ctcaaggcca ggataagtaa gacgatgcct
     1681 ccggagacga tggaccgcct gataaaggcg cgatcgctcc cggaggccgt tcagtttcta
     1741 aggggtaccg cctaccaggc gatcgaggaa gcctatacgg cgaccggcga cttgcgggcc
     1801 ggcgaggccc tgctccacga gcgcgagctc cgcctgtata ccggcctgtt ccggtacctg
     1861 gacgagccgg tcctgtcgtt cgtacgggcg ctcgcggcgc gctacgagat agaccaggtc
     1921 aagaacgccg tccgcttatg gttcgacgcc cacgtccggg gacgcgacgt ggccggtaag
     1981 tccggctacc tgttccgcgg gaccatcgtg aaccggatgg atgttgacgc tatcgtacgt
     2041 gccgccgata tcgagggcgt cgccgcggcg ctatcgggca ctccttacgc ggccatagcg
     2101 cgcgccgagc tacccgcggc ccaggcgtcc cgctccgtgt tctcgttcga gctggcgctc
     2161 gaccgccgct actacgacga agccttcgcg gcggcggcca gcctgggcgg ccgcgaccgg
     2221 gaagccgccg agcgcgccct cggcgtcgac gtggacctac agaacgtatc gtggctgata
     2281 cggttcaagg acttctacgg catgggcgcc gaagacgcca tgagcaggct gataccccga
     2341 ggccgcgcta tcaagccggc ggccgtggcg gcggtatggg atagcgagcg tggttcgcgc
     2401 ggagcgggcg gcataggctt ggacctactc ggccgctcgt atccgggcgt cggcgcgatg
     2461 atgggcggcg gcggttcggc ggccaagttg gccgtgctcg aggccgccat acggcgcgtc
     2521 gccgacgacg aggccgggaa gagcctctgc ggctatccgt tctcgatagg cgtcgtgctg
     2581 gcgtacttca ccaagaagcg cgccgagatg cgcgccgtaa tgactgtcct gaacgccaag
     2641 tactacgcgc tcgccgaaga aaggatacgg agctccctat gatgttcacc tctccgatga
     2701 gactgctcgc ggcgatggtc ctggacgcgg attcggacgc catcgccaag gagctcctgc
     2761 gcctcggggc gctcgagatg gtatcgatca aggaactgcc cggcgactgg gcctcgaggc
     2821 tcggcaaggc cgagcccaag gtcaacct
//
LOCUS       DAOPHV010000345         2833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_49513_length_2833_cov_5.165587, whole genome shotgun sequence.
ACCESSION   DAOPHV010000345 DAOPHV010000000
VERSION     DAOPHV010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49513_length_2833_cov_5.165587"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..2088)
                     /locus_tag="PLQ29_09155"
     CDS             complement(115..2088)
                     /locus_tag="PLQ29_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86852.1"
                     /translation="MSMAMSDAVIERIADRIAAERGDGCRERALEGVRCAAPLWRDED
                     GTQTDLEAFCLRQFCATDAERDALFLRLESVLEELYGSALALSRTFSWGVHVDRGPVL
                     PVDAILAEWSPYAHFSDDFYGNKLAFVIHLNFPYKDLDRKLRDGPSWTRHQWAEARLG
                     DQFLDRVPSEASQRLAAAAAEAETYIAGYYLYPSRLRDASGAAGSPAYSFPADKRLLM
                     HWNVRDEIKALYADPAGLPGQRLLYKAMERVVEGAIPRAFVDSRELTWDPFSGEAHTA
                     DGSRANPEREGDGRYDRLLAIFRAERGVDQWCPETPTLIARKFGVEREIPERQVERLF
                     VDALSAPLFGRAADELRRRLGRPLEAFDAWYKGFGDDSSPDEAALDAITRARYPDAKA
                     FKADIPRILGALGFDADLARWLSERIEVDQSRGSGHAMPAAKRSDSVRLRTRVPATGM
                     DYKGYNIACHELGHNVEQVLSLHGVDEYFLAGVPNNAFTEAFAFVFQARDRMLLGLEG
                     APSSPSALGDYWSTCEIAGVGLVDMEVWRWMYAHPDCTARDLREATMAIARSVWNRYF
                     APAYGLKDALILGTYSHMVELSMYLPDYPLGHLVAFQIEAAMAGKPIGPEMSHMCRLG
                     RLAPNQWLREATGSELSAEPLIAAAERELAAAR"
     gene            2222..>2833
                     /locus_tag="PLQ29_09160"
     CDS             2222..>2833
                     /locus_tag="PLQ29_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86853.1"
                     /translation="MPRTEKTRIYLLACVIASMTLLGATPEPPFEPLEHIPAGAVAVA
                     SVGDLTSLLSNSIGFMRNAGLGSEAAELSTLIDGALHPEGADSTDAAALFKAIDPGRR
                     VVAAVYLSEGDSGAAVLLFLPLRSSLNDEVRLAIERAARALSGEDQPPSIAVDYPGYA
                     AIRFDGVAIPAYGSGSTMRLGSLKAQAPSSLAVWVDPDGQAGRR"
BASE COUNT          484 a          963 c          950 g          436 t
ORIGIN      
        1 gccataccga cgagccagct tcgtataccg actgatatat caatatactg cttactgatc
       61 agagcgccta gcggagccgt acgaaccggc aagcgccgca actcccggcg agcgctacct
      121 ggcggcggcc agttcccgtt cggccgccgc gatcaacggc tccgccgaca gctcggagcc
      181 ggtagcctct cgcagccact gattcggcgc cagacggccg agcctgcaca tgtggctcat
      241 ctccgggcct atcggcttgc cggccattgc ggcctcgatc tggaaggcga cgagatgacc
      301 gagcgggtaa tcgggcaggt acatcgacag ctcgaccatg tgcgagtacg tgccgaggat
      361 cagcgcgtcc ttaaggccgt aggccggggc gaagtagcgg ttccagaccg aacgcgcaat
      421 cgccatcgtc gcctcgcgga ggtcgcgggc cgtgcagtcg ggatgggcgt acatccagcg
      481 ccagacctcc atgtcgacga gaccgacgcc cgctatctcg caggtactcc agtagtcgcc
      541 aagggccgag ggcgacgatg gagccccctc gaggccgagc aacatgcggt cgcgggcctg
      601 gaacacgaac gcgaaggcct cggtgaacgc gttattgggc accccggcca ggaaatactc
      661 gtcgacgccg tgcagggaca gcacctgctc cacgttgtgg ccgagctcgt ggcacgcgat
      721 gttgtagccc ttgtagtcca tgccggtggc cggcacccgg gtgcgcaggc ggacggagtc
      781 ggagcgcttg gccgccggca tggcgtgtcc cgagccgcgc gactggtcga cctcgatgcg
      841 ctcggacagc cagcgggcca ggtcggcgtc gaaaccgagc gcgccgagga tgcgcgggat
      901 atccgccttg aaggccttcg cgtcgggata acgcgcccgc gtgatagcgt cgagggcggc
      961 ctcgtcgggc gatgagtcgt cgccgaagcc cttgtaccag gcgtcgaagg cctcgagggg
     1021 gcggccgagg cgacgacgga gctcgtccgc cgccctgccg aacagcggcg ccgacagcgc
     1081 gtcgacgaat aagcgctcga cctggcgctc gggtatctcg cgctcgacac caaacttgcg
     1141 ggcgatgagg gtcggcgtct cggggcacca ctggtcgacg ccgcgctcgg cccggaagat
     1201 ggcgagcagg cgatcgtagc gcccatcgcc ctcgcgctcg ggattggccc tggaaccgtc
     1261 ggcggtatgt gcctcgccgg agaacgggtc ccaggtcagc tcgcgcgagt cgacgaaggc
     1321 ccgcgggatc gcgccttcga cgacgcgctc catcgccttg tacaggagcc gctggccggg
     1381 caggcccgcc ggatcggcgt acagcgcctt gatctcatcg cgcacgttcc agtgcatcag
     1441 gaggcgctta tccgccggga agctatatgc cggggagccg gcggctccgg acgcgtccct
     1501 gagcctcgac gggtacaggt agtagccggc gatataggtc tcggcctcgg ccgcagcggc
     1561 ggcgagccgc tgcgaagcct ccgacggcac gcggtccaag aactggtctc cgagcctggc
     1621 ctcggcccat tgatgccggg tccacgaagg tccatcgcgc agcttacggt cgagatcctt
     1681 gtacgggaag ttcaggtgaa tgacgaaggc gagcttgttg ccgtagaagt cgtcgctgaa
     1741 atgggcgtag ggcgaccatt ccgcaaggat cgcgtccacc ggcaggaccg ggccccggtc
     1801 gacgtggacg ccccaggaga aggtcctgga cagggcgagg gccgagccgt acagctcctc
     1861 gaggaccgac tcgagcctca ggaacaacgc gtcgcgctcg gcgtccgtag cgcagaactg
     1921 ccgcaggcag aacgcctcga ggtcggtctg cgtgccgtcc tcatcgcgcc agaggggcgc
     1981 cgcgcagcgt acgccctcga gcgcccgctc cctgcaacca tcgcctcgtt cggccgcaat
     2041 cctgtccgct atacgctcaa tgaccgcgtc gctcatcgcc atactcatcg tcgacctcct
     2101 ggcaccggcc ctatcctata ccggaggcgg ccagcgggca agcgacggcc cgctggatac
     2161 ttcgggtccg gtacggtatt cttttcttaa ttaatgatcg atagcacgga ggaacgccag
     2221 catgccccgc actgagaaaa cgcgcatcta cctactagcc tgcgtcatcg cctcgatgac
     2281 gcttttaggc gccacacccg agccgccgtt cgaaccgctc gagcacatcc ccgcgggggc
     2341 cgtcgcggtg gccagcgtcg gcgacctgac gtcgctcctg agcaactcga taggcttcat
     2401 gcggaacgcc ggtctcggga gcgaagccgc cgagctatcg acgctcatag acggcgcgct
     2461 gcatccagaa ggcgccgatt ccacggacgc ggccgccctg ttcaaggcga tcgaccccgg
     2521 caggcgcgtc gtggcggccg tctatctatc cgaaggcgat agcggggccg cggtgctact
     2581 cttcctgccg ctacgatcgt ccctaaacga cgaggttcgc ctcgcgatcg aacgggccgc
     2641 gagggccctg agcggagaag accagcctcc gagcatcgcc gtcgactacc cgggctatgc
     2701 cgcgatccgc ttcgacggcg tcgctattcc cgcctatggc tccggatcga cgatgaggct
     2761 cgggtcgctc aaggcgcagg cgccgtccag cctggccgtc tgggtcgacc cagacggcca
     2821 ggctggacgg cgc
//
LOCUS       DAOPHV010000346         2831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_49573_length_2831_cov_3.534582, whole genome shotgun sequence.
ACCESSION   DAOPHV010000346 DAOPHV010000000
VERSION     DAOPHV010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49573_length_2831_cov_3.534582"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="PLQ29_09165"
     CDS             complement(<1..1245)
                     /locus_tag="PLQ29_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG86854.1"
                     /translation="MAPSRAGLLWIFIRPYVDSDVRLFKKAKLLAPVLVAIAALAVIL
                     ATLMAVTGAVIVAAALTALIGLCVVSLVLLARGRYGASSTVFIYSLFAVMFGAIKFDA
                     YQNVYETYVFGTLGGFMLVTTGLIAAKPVQAWIMTLLNLLAIGALYYLDALPLDGGVV
                     TELAMQNLGTCGVIVIAAGIFSSMAIKMQSELVDDTRRSADAARIQFERMAAAVSDAQ
                     AGALEIGTRLAGAADTVSSTAASMRDVSSEELSGLASLIDALAAAEDGEKVADAAQDR
                     VSSSLEAYSAKVLEASASIAQMVEAVDGIGKAASERLEVVAKLVDMVKAGDERVTRIG
                     EAIADIVSATSKMDEMNGLIGQVAERTNMLGMNAAIEAAHAGDAGKGFAVVAEEIQSL
                     SDTAAEGSGSIASILSETAEVVA"
     gene            1335..1961
                     /locus_tag="PLQ29_09170"
     CDS             1335..1961
                     /locus_tag="PLQ29_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPG86855.1"
                     /translation="MLIVPYGLARVELTVVNSRFIASLAPADSVEAARAYIAGIRAEF
                     PDATHNVPAFIIGGGNAVTEFCSDDGEPSGTSGRPLLAALKGSGLGNAVVVVTRYFGG
                     TLLGTGGLVKAYSEAGKAVLAVARRAELVETRRLVFEAPYHLFERVRSLAEASRATIV
                     AEDFSESVALELDVAAADCDAFLSALADLSSGSIAPRLGSSRLAARPL"
     gene            complement(1992..2618)
                     /gene="msrB"
                     /locus_tag="PLQ29_09175"
     CDS             complement(1992..2618)
                     /gene="msrB"
                     /locus_tag="PLQ29_09175"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HPG86856.1"
                     /translation="MITRIASRGRAASAACAFCAYAVVSCGGVPSTQSGKPATAPIET
                     GKETNAMPVNTPMNAANVCYVEPSEELRARLSPEAYAVLVESATEPPFSNEYWDNHRP
                     GVYVDAIDGAPLFASLAKYDSGSGWPSFWAPIDPGRLVLVEDRSYGMRRIEVRAKASG
                     GHLGHLFDDGPEPTGLRYCINSASLRFVPLEGLEAEGYGELAALFSTP"
BASE COUNT          462 a          925 c          953 g          491 t
ORIGIN      
        1 ggcgaccacc tcggcggtct cggacagtat cgaggctatc gatcccgagc cctccgccgc
       61 agtatccgag aggctctgga tctcctcggc caccaccgcg aaacccttgc cggcgtcgcc
      121 cgcatgggcg gcctctatgg ccgcgttcat cccgagcatg ttcgtgcgct cggcgacttg
      181 gccgatcaag ccgttcatct cgtccatctt ggaggtggcc gacacgatgt cggctatagc
      241 ctcacctatc ctggtcacgc gttcgtcgcc ggccttgacc atgtcgacga gtttcgcgac
      301 gacctcgagc cgctccgagg cggccttgcc tatgccgtcc accgcctcga ccatctgagc
      361 tatcgaagcg gatgcctcga gtaccttggc cgagtaggcc tcgagcgacg agctgactcg
      421 gtcctgggcc gcgtcggcca ccttttctcc gtcctcggcg gccgcgaggg cgtcgatgag
      481 cgaagccagg ccggacagct cctcggagga gacgtcacgc atggacgccg ctgtactcga
      541 gaccgtgtcg gccgcgcctg ccaggcgcgt gcctatctcg agggcaccgg cctgggcgtc
      601 gctgacggcg gcggccatgc gctcgaactg gatcctggcc gcgtcggcgg agcggcgcgt
      661 gtcgtcgacc agctccgact gcatcttgat cgccatcgag ctgaagatgc ccgcggcgat
      721 gacgatgacg ccgcaggtgc ctaggttctg catggccagc tcggtgacca ctccgccgtc
      781 gaggggcaac gcgtctaggt agtagagcgc cccgatggcg agcaggttaa ggagcgtcat
      841 gatccaagcc tggactggct tggcggcgat caagccggtg gtaacgagca tgaagccgcc
      901 gagcgtgccg aagacgtagg tttcatagac attctggtag gcgtcgaact tgatggcgcc
      961 gaacatgacg gcgaacaggg agtagatgaa gacggtcgac gaggcgccgt agcggcccct
     1021 ggccaggagt accagcgaca cgacgcagag gccgatcagg gccgtcaggg cggccgctac
     1081 gatgacagct ccggttaccg ccatcagggt cgctaggatg accgcgagcg cggctatggc
     1141 tacgaggacc ggagccagga gcttggcttt cttgaagagc cgcacgtccg agtcgacgta
     1201 cggacggatg aaaatccata aaagtccggc gcgcgatgga gccatataca cctcatagtt
     1261 taaagctacc tatagtatag cgtatgatcc ggatgagaca agctttccga gggctagccg
     1321 tgctatagtg gggcatgctg atcgttccgt acggcttggc cagggtcgag ctgaccgtgg
     1381 tcaactcgcg cttcatagcc agcttggctc cggccgattc ggtcgaggcc gcccgcgcct
     1441 atatcgccgg tatacgtgcc gaattcccgg acgcgaccca caacgtgccg gccttcatca
     1501 tcgggggcgg caatgccgtt accgagttct gctccgacga cggcgagccg tccggtacct
     1561 cgggccggcc acttctggcc gcgctcaagg gctcggggct cggcaacgcc gtcgtcgtgg
     1621 tcacgagata tttcggcgga acgctgctcg ggaccggcgg cctggtcaag gcctactcgg
     1681 aggctggcaa ggccgtgctc gcggtcgcac ggcgggccga gctcgtggag actcgtcgcc
     1741 tcgtcttcga ggcgccgtac cacctgttcg agcgcgtacg ttcgctcgcc gaggcgagcc
     1801 gcgcgaccat cgtagcggag gatttctcgg agagcgtcgc cctggagctc gatgtggccg
     1861 cggcagactg cgacgcgttc ctatcggccc tggccgactt gagctccggc tcgatagccc
     1921 cgaggctcgg ctcttcgagg ctcgcggccc ggccgctcta gccggccgcg gccagcgact
     1981 cggccgccga gctagggcgt cgagaatagc gccgccagct cgccgtagcc ctcggcttcc
     2041 aacccttcga gcggtacgaa acgtagcgac gccgaattga tgcagtaccg cagcccggtc
     2101 ggctcgggcc cgtcgtcgaa caggtgcccg aggtggccgc ccgaggcttt ggcccgtacc
     2161 tctatcctcc tcatgccata ggaccggtcc tcgacgagga ccaggcggcc ggggtcgatc
     2221 ggggcccaga agctcggcca gccggagccg gagtcgtatt tggccagcga ggcgaatagc
     2281 ggcgcgccgt cgatcgcgtc gacgtagacg ccgggcctgt ggttatccca gtactcgttg
     2341 ctgaatggcg gctcggtggc gctctccacg agcacggcgt aggcctcggg cgacagcctg
     2401 gcccgtagct cctcgctcgg ctcgacgtag cagacgttgg cggcgttcat cggcgtattg
     2461 acgggcatgg cgttcgtttc ctttccggtc tcgataggag cggtggccgg ctttcctgac
     2521 tgcgtcgacg gtacgccgcc gcatgagacc acggcgtacg cgcagaaggc gcaggcggcc
     2581 gaggccgcgc ggcccctcga ggctatgcgc gttatcatga atataatata gggaatttgc
     2641 tatgaaaggt gaatacggag ggcgcgtcga agcgcctcag cttccgatga acagctccgc
     2701 tcggaacctg gccttagcgt ccgccgagcc ggccgacagg gagcccgcag ggcgcccctc
     2761 gacggcgtag cccttggccc agccgtccga cgggccgagc gccttggcgt agatgtcgcc
     2821 ggcctcgccg g
//
LOCUS       DAOPHV010000347         2825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_49729_length_2825_cov_3.252347, whole genome shotgun sequence.
ACCESSION   DAOPHV010000347 DAOPHV010000000
VERSION     DAOPHV010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49729_length_2825_cov_3.252347"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PLQ29_09180"
     CDS             complement(<1..964)
                     /locus_tag="PLQ29_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG86857.1"
                     /translation="MNTLANIALRNLTRQKKRTVLLGSAIAFGVMIVTLINGFAGAFS
                     ANVAENFSDLMAGHVFVSGSEKGEGDKTLSVIRDDSALTAAVEASGAPVRYVTRRSSF
                     AGTLIFEGKKLQTNVDGADLSQETFLRDRLLLKEGSWDSMSDENALILSEKIASRLNV
                     DVGDLILVQLETVTGQANVGEFRLAGVSYDAGIFGQMMSYANLGYVNSLIGLAPDEYQ
                     TMGIMLDGLKHTDAAAEAIYGRLYGGVQLFERGVKDEDGAVTPFQALMRQQKKESWEG
                     VKYRLFTINEVLSQIEQIVAALDTTAGVILVVLFVIIMVGITNTF"
     gene            complement(977..2497)
                     /locus_tag="PLQ29_09185"
     CDS             complement(977..2497)
                     /locus_tag="PLQ29_09185"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPG86858.1"
                     /translation="MPVTLRMAFRNLREHKAKSLIIGILLALGMLIMVLGNSFLDASK
                     RGIERSFTQNYTGDVIITGVADGPVSLFGVQSVGGMEQTPIIPDYEKVFAHVSGLRGV
                     ESMAGMASGYGIATNEAGEIAEDEDEDDEERMEGMMAALMMLFGVDAPGYWELFDTIE
                     LVDGEFIQPGRPGLMMADDRVAKLAEYLKRDIRVGDEILIQGMGDAGIRLRSVPLVGT
                     FKRVTDGAGPEQLTYIDIDTLRALSGMTIGASENIELGEAETSLLALDDLDALFGDDD
                     AFGVEAAPGSGAALTEAGIEGLLGDTSSRERLNAADTGSWHSILLRLDDPARTRAVAA
                     ELNAWFAMEGIQAQAGDWQKAAGPYAQSVDVLRIVFTVAIVILAVVAIIIIMNTLVVS
                     IIERTGEIGTMRALGGGKGFVRRLFAAETVALSLFFGFVGMVLSFLAAAVINAVGFEA
                     SNEFLRILFGGSVLRAVIDPLALLWSLAVIVAVGLVASVYPVSVALRIQPVRAMQS"
     gene            complement(2500..>2825)
                     /locus_tag="PLQ29_09190"
     CDS             complement(2500..>2825)
                     /locus_tag="PLQ29_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG86859.1"
                     /translation="SEWRKHKPKELSGGQRQRVAIARALVTRPQIVLADEPTANLDSA
                     TGEQILELMKKINRELETTFIFSTHDARIVDIADHIIRLRDGLVTENRRREEEAPAAC
                     QGAEA"
BASE COUNT          452 a          912 c          960 g          501 t
ORIGIN      
        1 tgaaggtgtt ggtgatgccg accatgatga tcacgaacag cacgaccagg atgacgccgg
       61 ccgtggtgtc gagggcggcg acgatctgct cgatctggga gagcacctcg ttgatcgtga
      121 acaggcggta tttgacgccc tcccagctct ccttcttctg ctggcgcatc agggcctgga
      181 acggcgtcac ggcgccgtcc tcgtccttga cgccgcgttc gaacagttgc acgccgccgt
      241 acaggcggcc gtagatcgcc tccgccgccg cgtcggtgtg cttgagaccg tcgagcatga
      301 tgcccatggt ctggtactcg tccggcgcga ggccgatcag gctgttgacg tagccaaggt
      361 tggcgtagga catcatctgc ccgaagatgc cggcgtcgta gctgacgccg gcgagcctga
      421 actcgccgac gttggcctgg ccggtcacgg tctcgagctg gaccaggatg aggtcgccga
      481 cgtcgacgtt gaggcgcgag gctatcttct cggacaggat caacgcgttc tcgtcggaca
      541 tcgagtccca tgagccttct ttcaggagca agcggtcgcg caggaaggtc tcctgcgaca
      601 ggtccgcccc gtcgacgttg gtctgcagct tcttgccctc gaagatcagg gtgccggcga
      661 acgacgagcg cctggtcacg tagcggaccg gcgcgccgga ggcctcgacg gccgcggtca
      721 gggcgctgtc gtcccggatg accgacagcg tcttgtcgcc ctcgcccttc tcggagccgg
      781 agacgaagac gtggccggcc atcaggtcgg agaagttctc cgcgacgttg gccgagaagg
      841 ctccggcgaa gccgttgatc agggtgacta tcatcacgcc gaaggcgatg gcgctgccga
      901 gcagcacggt gcgcttcttc tggcgggtca ggttccgtaa ggctatgttt gccaaggtat
      961 tcaaagcggt atccccttac gattgcatcg cccgtacggg ctggatgcgc agcgcgacgc
     1021 tgaccgggta gacgctcgcg acgaggccga cggccacgat cacggcgagc gaccacagga
     1081 gcgcgagcgg gtcgatgacg gccctgagga ccgagccgcc gaacaggatc ctcaggaact
     1141 cgttcgaggc ctcgaagccg acggcgttga tcacggcggc cgctagaaag gacaggacca
     1201 tgccgacgaa gccgaagaac agcgacagcg ccacggtctc ggccgcgaac agcctgcgca
     1261 cgaagccctt gccgccgccg agcgcccgca tggtgccaat ctcgccggta cgctcgatga
     1321 tcgagacgac cagggtgttc atgatgatga tgatcgcgac gacggcgagg atgacgatgg
     1381 ccaccgtgaa gacgatgcgc agcacgtcga ccgactgggc gtacggcccc gccgccttct
     1441 gccagtcgcc ggcctgggcc tggatgccct ccatggcgaa ccaggcgttg agctcggccg
     1501 ccacggccct ggtgcgggcc gggtcgtcga ggcgcaggag tatcgagtgc caggagccgg
     1561 tgtcggccgc gttgagccgc tccctggagc tggtatcgcc gaggaggcct tctatgccgg
     1621 cctcggtcag ggcggcgccc gagccaggtg cggcctcgac gccgaaggcg tcgtcgtcgc
     1681 cgaacagcgc gtcgaggtcg tcgagcgcga gcagcgacgt ctcggcctcg ccgagctcga
     1741 tattctcgga agcgcctatc gtcatgccgg acagcgcgcg cagcgtgtcg atgtcgatat
     1801 aggtgagctg ttccgggccc gcgccgtcgg tcacgcgctt gaaggtgccg acgaggggca
     1861 cgctgcgcag gcgtatgccc gcgtctccca tcccctggat gagtatctcg tcgcctaccc
     1921 gtatgtcgcg cttgaggtac tcggcgagct tggccacgcg gtcgtcggcc atcatgaggc
     1981 cgggccggcc gggctggatg aactcgccgt cgaccagctc tatcgtgtcg aaaagctccc
     2041 aatagccggg ggcgtcgacg ccgaagagca tcatcaaggc ggccatcatg ccctccatgc
     2101 gctcctcgtc gtcctcgtcc tcgtcttccg ctatctcgcc ggcctcgttc gtggcgatgc
     2161 cgtagcccga ggccatgccg gccatcgact cgacgccccg caggcccgag acgtgggcga
     2221 agaccttctc gtagtccggg atgatgggcg tctgctccat gccgccgacc gactgcacgc
     2281 cgaacagcga caccgggccg tccgcgacgc cggtgatgat cacgtcgccc gtgtagttct
     2341 gggtgaacga tctctctatg ccccgtttcg aggcgtccag gaaggagttg ccgagaacca
     2401 tgatgagcat gcccagggcc aggagtatgc ctatgataag gctcttggcc ttgtgctccc
     2461 tcaggttccg gaacgccatg cgcagcgtta cgggcatggt caggcctcgg cgccctggca
     2521 ggcggccggc gcttcctctt cgcggcggcg gttctcggtc acgagtccgt cgcgtagcct
     2581 gatgatgtgg tcggctatgt cgacgatgcg cgcgtcgtgg gtactgaaga tgaaggtagt
     2641 ctcgagctcg cggttgatct tcttcatgag ctcgaggatc tgctcgccgg tcgcgctgtc
     2701 caggttggcg gtcggctcgt cggccagcac gatctgcggc ctggtaacca gggcccgcgc
     2761 gatggcgacg cgctgcctct ggccgccgga cagctccttc ggcttgtgct tgcgccactc
     2821 ggaca
//
LOCUS       DAOPHV010000348         2819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_49888_length_2819_cov_3.445731, whole genome shotgun sequence.
ACCESSION   DAOPHV010000348 DAOPHV010000000
VERSION     DAOPHV010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49888_length_2819_cov_3.445731"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /gene="chvE"
                     /locus_tag="PLQ29_09195"
     CDS             <1..776
                     /gene="chvE"
                     /locus_tag="PLQ29_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPG86860.1"
                     /translation="DVLKKAKASGIKVIAYDRLIMNTPDVDYYATFDNFKVGVIQGRY
                     IESALGLALGKGPFNIELFAGSPDDNNAVFFNKGAMSILQPYLDKKQLVVPTAQTEFT
                     TIAIQSWSSEKAQARMDNLIAMKYSRKTVLDAILSPNDSLAIGIVASLKNAGYGTAKK
                     PYPVLTGQDCDKANVKAMIAGQQSMSIFKDTRTLAAQVVAMVEAIMKGKEAPVNDTKT
                     YNNGVKVVPSYLCDPVYADKFNYRTLLIDSGYYTEADLK"
     gene            863..2413
                     /gene="gguA"
                     /locus_tag="PLQ29_09200"
     CDS             863..2413
                     /gene="gguA"
                     /locus_tag="PLQ29_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPG86861.1"
                     /translation="MGDTILEMRNIVKTFPGVRALNDVTLSVRRGEIHALVGENGAGK
                     STLMNVLGGVYPHGSYEGQLVFDGEECSFRTIHDCEKKGIVIIHQELALIPYLSVGEN
                     IFLGNERASRGVIDWDGTHREAERLLQLVGLKEGTRTLIKDISVGKQQLVEIAKALSK
                     NVRLLVLDEPTASLNEDDSEHLLKLLLDLKAKGVTSILISHKLNEVVEVADRLTILRD
                     GSTIETLDKESCSIDEDRIIKGMVGRELVDRFPKRTPKIGEVAFEIKGWNVYSPLNPE
                     RRIIKDVSLEVRRGEVVGLAGLMGAGRTELAMSVFGRSYGARISGRAFKDGKEIELKR
                     VRDAIDQGISYATEDRKSAGLNLIGDIRENTTIAALKKVARRLVIDRDKEAGVAEDYR
                     ARLKIRSTSIRQKAGNLSGGNQQKVVLSKWIFTAPDVLILDEPTRGIDVGAKYEIYTI
                     INKLADEGKSVIFISSELPEILGVSDRVYVMNEGRMVGEFDRGEATQEGIMKCIMQSA
                     RENGNGKH"
     gene            2400..>2819
                     /locus_tag="PLQ29_09205"
     CDS             2400..>2819
                     /locus_tag="PLQ29_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPG86862.1"
                     /translation="MGSIKDFLRNNIRQYAMLIALVFIMIFFQITTEGVLLVPMNVTN
                     LVLQNAYVFILAIGMTLCILTGGNIDLSVGSVVAFVGALCGTFIVTMKLSVGVGITFA
                     LVIGLAIGVWQGFWIAYVRLPAFIVTLAGMLTFRGLTT"
BASE COUNT          604 a          890 c          865 g          460 t
ORIGIN      
        1 cggacgtgct caagaaggcc aaggcgagcg gcatcaaggt catcgcctac gaccgcctga
       61 tcatgaacac gccggacgtc gactactacg ccacgttcga caacttcaag gtaggcgtga
      121 tccagggccg gtacatcgag tccgccctcg gcctggccct gggtaaggga cccttcaata
      181 tcgagctgtt cgccggctcg cccgacgaca acaacgcggt cttcttcaac aagggcgcga
      241 tgagcatcct ccagccctac ctggacaaaa agcagctcgt cgtcccgacc gcccagaccg
      301 agttcaccac catcgccatc cagagctgga gttccgagaa ggcccaggcc aggatggaca
      361 acctgatcgc gatgaagtat tcccgcaaga cggttctcga cgcgatcctg tcgccgaacg
      421 acagtctggc gatcggcatc gtcgcatcgc tcaagaacgc cggctacggc acggccaaga
      481 agccctaccc ggtgctgacc ggccaggact gcgacaaggc caacgtcaag gcgatgatag
      541 ccgggcagca gtcgatgtcg atcttcaagg acacgaggac cctggcggcc caggtcgtcg
      601 cgatggtcga ggcgatcatg aagggcaagg aagcccccgt caacgacacc aagacctaca
      661 ataacggcgt caaggtcgta ccgtcctatc tctgcgaccc cgtctacgcg gacaagttca
      721 attatcggac cctgctcatc gatagcggct actataccga agccgacctg aagtaagagc
      781 acgtaaccct agccgggcgc cggcccgcgg acctccccgc gggccggcct ttagtaacca
      841 ggctacgcga ggagaagcta ccatgggcga caccatcctc gagatgcgca acatcgtcaa
      901 gaccttcccc ggcgtccgag cgctgaacga cgtgacgctc tcggtacggc gcggcgagat
      961 ccacgccctc gtcggcgaga acggggccgg caagtccacc ctgatgaacg tcctcggcgg
     1021 cgtctaccct cacggcagct acgaggggca gctcgtcttc gatggcgagg agtgctcctt
     1081 ccgcaccatc cacgactgcg agaagaaggg gatagtcatc atccaccagg agctcgcgct
     1141 gatcccctac ctatccgtag gcgagaacat cttcctgggg aacgagcgcg cgtcgcgggg
     1201 ggtgatagac tgggacggca cgcatcgcga ggccgagcgg ctcctccagc tcgtcggcct
     1261 caaggagggc acgcgtaccc tgatcaagga tatcagcgtc ggcaagcagc aactcgtcga
     1321 aatcgccaag gcgctgtcca agaatgtcag gctgctcgtg ctcgatgagc cgacggcgtc
     1381 gctcaacgag gacgactcgg agcatctgct caagctcctc ctcgacttga aggccaaggg
     1441 cgtcacctcc atcttgatat cgcacaagct gaacgaggtc gtcgaggtcg cggaccggct
     1501 gacgatactc cgcgacggct cgacgataga gaccctcgat aaagagagct gtagcatcga
     1561 cgaggatcgc atcatcaagg gcatggtcgg ccgggagctg gtcgaccgct tcccgaagcg
     1621 gacgccgaag ataggcgagg tcgccttcga gatcaagggc tggaacgtct atagcccgct
     1681 caatcccgag cggcggatca tcaaggatgt gagcctcgag gtacgcaggg gcgaggtcgt
     1741 cgggctggcc ggcctgatgg gagcgggcag gaccgagctg gcgatgagcg tcttcggccg
     1801 ctcctacggc gcccggatct ccggacgggc cttcaaggac ggcaaggaga tagagctcaa
     1861 gcgggtccgc gacgccatcg accagggcat atcctacgcc accgaggacc gcaagtcggc
     1921 cggcctcaac ctgatcgggg acatacgcga gaacacgacg atcgccgccc tcaagaaggt
     1981 ggccaggcgg ctcgtgattg accgcgacaa ggaggcgggc gtagccgagg actaccgagc
     2041 cagactcaag atacgctcga cgagcatacg gcagaaggcg ggcaacctgt ccggcggcaa
     2101 ccagcagaag gtcgtcctgt ccaagtggat cttcaccgcg cccgacgtgc tgatcctgga
     2161 cgagccgacg cgcggcatcg acgtcggggc caagtacgag atttacacca tcatcaacaa
     2221 gctggccgac gagggcaagt cggtcatctt catctcttcc gagctgcccg agatactcgg
     2281 cgtctccgac cgcgtctacg tgatgaacga gggacggatg gtcggcgagt tcgatcgggg
     2341 cgaggcgact caggaaggca tcatgaagtg catcatgcaa agcgccaggg agaacggtaa
     2401 tgggaagcat taaggatttc ctccggaaca atatccggca gtatgcgatg ctcatcgctc
     2461 tcgtgttcat catgatcttc ttccagatca ccaccgaggg cgtgctcctg gtgccgatga
     2521 atgtgaccaa cctggttctc cagaacgcgt acgtattcat cctggcgata ggcatgacgc
     2581 tgtgcatcct gaccggcggc aacatagacc tgtcggtagg ctcggtcgtc gccttcgtcg
     2641 gcgccctctg cggaaccttc atcgtcacga tgaagctgag cgtcggcgtc ggcataacct
     2701 tcgcgctggt catcggcctg gccataggcg tctggcaggg cttctggata gcgtacgtgc
     2761 ggctaccggc cttcatcgtc accctggcgg gcatgctcac cttccgcggc ctgaccacg
//
LOCUS       DAOPHV010000349         2818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_49913_length_2818_cov_4.133189, whole genome shotgun sequence.
ACCESSION   DAOPHV010000349 DAOPHV010000000
VERSION     DAOPHV010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49913_length_2818_cov_4.133189"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..748
                     /locus_tag="PLQ29_09210"
     CDS             80..748
                     /locus_tag="PLQ29_09210"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPG86863.1"
                     /translation="MPRPVSERRVRGRIATGLFKPAGVPARAIEEIVLGLDEAEAIRL
                     ADLEGLYQEAAARRMGVSRQTFGRILEEAHRIVADALINGKALRIEGGQIIEQEEQHM
                     HEKVAVPTKAGMVDEHFGHCEYFTVYSIRDGKVVSEGRVDSPNGCGCKTDIASVLAKD
                     GVTLMLAGNMGEGAVRVLKANGIEVVRGASGQARAVVDAWLAGSVTDSGVGCSAHEGH
                     DCSH"
     gene            822..1679
                     /locus_tag="PLQ29_09215"
     CDS             822..1679
                     /locus_tag="PLQ29_09215"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPG86864.1"
                     /translation="MTYQQKDRGLGALALAGTSFLWSLAGLLIKLVDWNPFAIACGRS
                     VVAAVFLLLWIRKPKFTFSFPQLGAAVASAATMVLFIYANKATTAANAILLQYGSPIY
                     TAVLGAFLLKEKPRAEHWAAFAMVAGGMALFFMGDIGGGSLPGNIAAVASGLTFAAYF
                     VFMRMQKDGSPLESNLLAHAITAVIGFVVALFMPAPQFNLKAAAAIGALGVFQIGLAA
                     VLLSWGIKRVNAIQGSIITGLEPVFNPLWVFLALGEKPSPNALAGGAVIIAAVVGSSI
                     VTARRKTKA"
     gene            1996..2580
                     /locus_tag="PLQ29_09220"
     CDS             1996..2580
                     /locus_tag="PLQ29_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86865.1"
                     /translation="MKITRKRAPIIAAAIAALAVSAYAEGSGHGASAAIAALAASAYA
                     EGSGYGASAAVEAGRAITIVDMLRWAVEDEYLARGEYEAIMARFGAMRPYDNIMAAEE
                     QHLAWLKAEYQARGLPFPTDGSASSVVVPTDLKAAAQAGVDAEIANIAMYSSFLSRPE
                     LSRPENASVRDLFERLKRASENHLRAFKTQLSKY"
     gene            2611..>2818
                     /locus_tag="PLQ29_09225"
     CDS             2611..>2818
                     /locus_tag="PLQ29_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86866.1"
                     /translation="MASGASTKKPKKASRWIRRAVQILFFALVATIATNEILSERGIT
                     ALSFVEGASLHAICPFGGVVSFWQL"
BASE COUNT          502 a          944 c          887 g          485 t
ORIGIN      
        1 tcgtcgaggg cttggcgtcc gagacgcttc tcgcgctctc ctcggtcaag aaaccggccg
       61 ccggctcaaa ggagtagccg tgccgagacc cgtcagcgag cgccgcgtac gcggccgaat
      121 cgccaccggc ctgttcaagc cggccggcgt gcccgcgcgg gccatcgagg agatcgtgct
      181 cggcctcgac gaggccgagg cgatccggct ggccgacctg gagggcctgt atcaggaggc
      241 cgcggcgagg cgcatgggcg tctcgaggca gaccttcggc cgcatactcg aggaggcgca
      301 ccgcatagtc gcggacgcgc tcataaacgg caaggccctc cgcatcgagg gcggccagat
      361 catagagcaa gaggagcagc atatgcacga gaaggtagcg gtcccgacca aggctggcat
      421 ggtggacgag catttcggcc actgcgagta ctttaccgtc tactcgatac gggacggcaa
      481 ggtcgtctcc gagggacgcg tcgacagccc gaacggctgc ggctgcaaga ccgacatcgc
      541 gtcggtcctg gccaaggacg gcgtcaccct gatgctcgcg ggcaacatgg gcgaaggcgc
      601 ggtgcgcgtg ctcaaggcca acggcatcga ggtggtgcgc ggcgcatccg gccaggcccg
      661 ggccgtcgtc gacgcgtggc tcgccggctc ggtcacggac tcgggcgtcg gctgctccgc
      721 ccacgagggg cacgactgct cgcactgatc cgctgggaca ccccttcccg gctgttcggg
      781 ccgattgccc acgaccgccc ggggaggata ctatcccgcg catgacgtat caacaaaaag
      841 accgcggcct cggcgcccta gccctggcag gcacgtcctt cctctggagc ctcgcggggc
      901 tcctgatcaa gctcgtcgat tggaacccct tcgccatagc ctgcggacgc agcgtcgtcg
      961 ccgccgtatt cctattgcta tggattcgca agcccaagtt cacgttctcg ttcccgcagc
     1021 taggcgccgc cgtggcgagc gcggcgacga tggtcctatt catctacgcc aacaaggcga
     1081 cgacggccgc gaacgccata ctccttcagt acgggtcgcc gatctacacc gccgtcctcg
     1141 gcgccttcct cctcaaggag aaaccgaggg ccgagcactg ggcagccttc gccatggtcg
     1201 ccggcggcat ggcgctcttc ttcatgggcg acataggcgg cgggagcctg ccgggtaaca
     1261 tagccgcggt agcctcgggc ctgaccttcg cggcctactt cgtgttcatg cgcatgcaga
     1321 aggacggttc gccgctcgag tcgaacctac tcgcgcacgc gatcaccgcc gtcatcggct
     1381 tcgtcgtcgc gctgttcatg cccgctcctc agttcaacct caaggccgcg gccgctatcg
     1441 gcgcgctcgg cgtattccag atcgggctcg ccgcggtgct cctgtcctgg ggtatcaagc
     1501 gggtcaacgc catccagggc tccatcatca cggggctcga gccggtgttc aacccgctct
     1561 gggtgttcct ggccctcggc gagaagccga gcccgaacgc gctcgccgga ggcgcggtga
     1621 tcatcgcggc cgtcgtcggc tcatccatcg tcacggcgcg caggaagacc aaggcatagc
     1681 ggccgagcgc atcgcgtctt ctccactccg ccaccgtacc ccgattcgcc ttacgccaaa
     1741 gtgccccaag ccaccgtcgc cttctggcgt tcaggcacgt gcattttttt cacatcgcct
     1801 aacgtttctt tctcgatcgg atgaatactt agtcgcacat tccaccgccc gccaggcaca
     1861 gcctggtggg ccacatcgtt ttcatattgt atatcattat ataataatga catagaatga
     1921 gttcatacaa atctaatcaa cctggcacgc ttactgctat atagtaatta ctctatgccg
     1981 gttggggggg tgcctatgaa aatcactaga aaacgcgcgc ctataatcgc cgccgcgatc
     2041 gccgcgctgg ccgtatcggc ctacgccgag ggttcgggcc acggggcgag cgccgcgatc
     2101 gccgcgctgg ccgcatcggc ctacgccgag ggttcgggct acggggcgag cgccgcggtc
     2161 gaggctggcc gggcaatcac tatcgtcgac atgctccgtt gggcggtcga ggacgaatac
     2221 ctggctcgcg gcgaatacga ggccatcatg gctaggttcg gcgccatgag gccgtacgac
     2281 aacatcatgg cggccgagga acagcatctc gcctggctca aggccgagta ccaggcccgc
     2341 ggcctaccct tccccacgga cggctcggcc tcgagcgtcg tcgttcctac ggacctgaaa
     2401 gccgccgccc aggccggcgt cgacgcggag atcgccaaca tagcgatgta ctcgtccttc
     2461 ctgagccgcc ccgagctatc gcgccccgag aacgcgagcg tcagggacct gttcgagcgg
     2521 ctgaagcgcg cgagcgagaa tcacctgcgg gcctttaaaa cgcagctgtc caagtactag
     2581 actagaaacg atcaggagca aggaggcacc atggcaagcg gcgcgtcgac caaaaagccg
     2641 aagaaggcat cgcgatggat caggcgggcg gttcagatcc tgttcttcgc cctggtcgcg
     2701 accatagcga cgaatgagat actgagcgag cgaggcatca cggcgctgtc gttcgtcgag
     2761 ggcgcctcgc tgcacgcgat ctgccccttc ggcggcgtgg tctcgttctg gcagctcg
//
LOCUS       DAOPHV010000350         2815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_50004_length_2815_cov_3.326087, whole genome shotgun sequence.
ACCESSION   DAOPHV010000350 DAOPHV010000000
VERSION     DAOPHV010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50004_length_2815_cov_3.326087"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="PLQ29_09230"
     CDS             complement(<1..1395)
                     /locus_tag="PLQ29_09230"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPG86867.1"
                     /translation="MDPRTFLKGLHPLEIKVLLRYAPGDGLDAARLASELGYVEGHAN
                     QAQAWLSAKGLAAETGRSSRNVYELTALGREWLEAGSPEERMVAWLAQHGPAAMPTIC
                     QAVGIEQKDAGSAYGRLSKEGVLAMGQDKLVSLVDASKSLRAAALKALLAKAAAAGGI
                     LDEGTLGDAERALMAEVAKKRGAADAAFKVAERETVTWALTADAAAVSALLREEGVSG
                     DELGAVTQELLESGSWKGAAFRSYNIKSRPARLIPGRRNPYVQFLESVKDKLVSLGFE
                     EFDGGLVETEFWNGDALFMPQFHSARDIHDVYYVSEPTKAKAIEEPYLSRVATTHENG
                     GATGSRGWRYGFDREFTKRLVLRSQGTVLSARQLPTASVPGKYFGIARCFRYDKVDAT
                     HLSDFYQTEGIVLGEDVSLRTLLGFLEMFAIEVAGAKEVKYVPGYFPFTEPSVEVHIK
                     HPVLGWFELGGAGIF"
     gene            complement(1455..>2815)
                     /locus_tag="PLQ29_09235"
     CDS             complement(1455..>2815)
                     /locus_tag="PLQ29_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPG86868.1"
                     /translation="FEDMLMVPFSGVYDTYLIPNAHPDPERRLSQLCDAVRLIYASLY
                     STTARAYFDASGYKIEEERMAVVIQELVGTRRGRWYYPLISGTAQSYNYYPVSYLKPD
                     DGLCVAALGLGPYVVEGGEAHRFCPRYPKLDVVPPGRGLGGTQRWFYALDMERLDFDL
                     TQGEEATLSRLDVAEAEADPGFAMVASTWSVEDDRLEPGVSARGPRIVDFAPVLKYDS
                     FPFAEVVEAVLEVGTKSMGIPVEIEYAVSDDGPGGAPVFYMLQIKPLIQRSDRVSVDL
                     GAIVRGQCLVASERCMGNGRVRGVRDIVYVDPTRWNPAESGAVAAEIAGLNERLKAEG
                     RPYALVGPGRWGTRDHSLGVPVNFAQISNAKAIVEADLPDFRVDSSLGSHFFHNVTAM
                     NIGYFSAAYGSLSSFVDWDALAALPLVARTAHCSLVRAERELEILMDGGRGHGVIRPS
                     SD"
BASE COUNT          452 a          930 c          976 g          457 t
ORIGIN      
        1 gaagatgccg gcgccgccga gctcgaacca gccgagcacc gggtgcttga tgtgcacctc
       61 gaccgacggt tcggtgaacg ggaagtagcc gggtacgtac ttgacttcct tggcgccggc
      121 cacctcgatg gcgaacatct cgaggaagcc aaggagggtg cgtaggctca cgtcctcgcc
      181 gagcacgatg ccctcggtct ggtagaagtc ggacaggtgg gtcgcgtcga ccttgtcgta
      241 acggaagcat cgcgcgatgc cgaagtactt gcccggcacc gacgcggtcg gcagctgcct
      301 ggccgatagc accgtaccct ggctgcgcag cacgaggcgc ttggtgaact cgcggtcgaa
      361 gccgtagcgc cagccgcggg agccggtggc cccgccgttc tcgtgcgtgg tcgcgacgcg
      421 cgaaaggtag ggctcctcga tagccttggc cttggtcggc tcggagacgt agtagacgtc
      481 gtgaatgtcg cgggccgagt ggaactgggg catgaacaga gcgtcgccgt tccagaactc
      541 ggtctcgacg aggccgccgt cgaactcctc gaaaccgagc gagacgagct tgtccttgac
      601 gctctccagg aactgcacgt aggggttgcg gcgccccggg atcaggcgcg ccggccggct
      661 cttgatgttg tacgacctga acgcggcgcc cttccacgag ccggactcga gcagctcctg
      721 cgtgacggcg cccagctcat cgccggacac gccctcttcg cgtagcaggg ccgagacggc
      781 cgcggcatcg gccgtgaggg cccaggtgac tgtctcgcgc tcggcaacct tgaaggccgc
      841 gtcggccgcc ccgcgcttct tggccacctc ggccatcagc gcgcgctcgg cgtccccgag
      901 ggtgccttcg tccagtatcc cgccggcggc ggcggccttg gccagcaggg ccttgagcgc
      961 ggccgcccgg agcgacttgg acgcgtcgac gagcgagacg agcttgtcct gccccatcgc
     1021 caggacgcct tccttggaaa ggcggccgta ggccgagccg gcgtccttct gctcgatgcc
     1081 gacggcctgg cagatggtag gcatcgcggc cggaccgtgc tgggccagcc acgcgaccat
     1141 gcgctcctcg ggcgagcccg cctcgagcca ctcgcggcca agcgcggtca gctcgtagac
     1201 gttgcgcgac gagcggcccg tctcggcggc caggcccttg gcgctcagcc aggcctgggc
     1261 ttggttggcg tgcccctcga cgtagccgag ttccgacgcg agcctggccg cgtcgaggcc
     1321 gtctcccggc gcgtatctga gcagtacctt tatctcgagc gggtgcaagc ccttgaggaa
     1381 cgttcgtgga tccatgatgc cttctccagc gtctgctatt gttgctacga tagtgtcgga
     1441 attcgccgtc gtcgtcaatc ggaggacggg cgtatgacgc catgcccccg ccccccgtcc
     1501 attaggatct ccagttcccg ttcggcgcgg acgagcgagc aatgcgccgt acgcgcgacg
     1561 agcggcaggg ctgccagagc gtcccagtcg acgaaggacg atagcgagcc gtaggcagcg
     1621 gagaagtacc cgatgttcat cgcggtaacg ttatggaaga aatggctgcc gagcgacgag
     1681 tctaccctga agtccgggag gtcggcctcg acgatggcct tggcgttcga tatctgagcg
     1741 aaattaaccg gcaccccgag cgaatggtcg cgcgtacccc atcgtcctgg accgacgagg
     1801 gcgtaagggc gtccctcggc cttcagcctc tcgttcaggc cggctatctc ggccgcgacg
     1861 gccccgctct ccgctgggtt ccagcgcgtg gggtcgacgt acacgatatc gcggacgccg
     1921 cggacgcggc cgttgcccat gcagcgctcg ctcgccacga ggcactgacc ccgcacgatg
     1981 gcgccgaggt cgaccgagac ccggtccgat cgctggatca gcggcttgat ctggagcatg
     2041 tagaagaccg gcgccccgcc cggcccgtcg tcgctgacgg cgtattcgat ctccaccggt
     2101 atacccatgc tcttggtgcc gacctcgagg acggcctcga cgacctcggc gaaggggaac
     2161 gagtcgtact tgagcaccgg ggcgaagtcg acgatgcgcg gccccctggc ggagacgccc
     2221 ggctccagcc tgtcgtcctc gacgctccag gtgctggcga ccatcgcgaa gccgggatcg
     2281 gcttcggcct cggcgacgtc gagccgcgat agcgtggctt cctcgccctg agtcaggtcg
     2341 aagtcgagcc gctccatgtc gagggcgtag aaccagcgct gggtgccgcc gaggccccgg
     2401 cccggcggta cgacgtcgag cttggggtag cggggacaga agcgatgggc ttcgccgccc
     2461 tcgacgacgt acggcccgag tccgagggcg gccacgcaga gaccgtcgtc gggcttgagg
     2521 tacgagacgg gatagtagtt ataggactgg gccgtgccgg atatcagcgg gtagtaccag
     2581 cgcccgcgcc tggtgccgac cagctcctgg atcacgaccg ccatacgctc ctcctcgatc
     2641 ttgtagccgg acgcgtcgaa gtaggcgcgg gcggtcgtcg aatagaggct ggcgtagatg
     2701 agccggacgg cgtcgcagag ctgcgaaagg cgccgctcgg ggtcgggatg ggcgttcggg
     2761 atcaggtagg tatcgtacac gccggaaaac ggcaccatga gcatgtcctc gaaca
//
LOCUS       DAOPHV010000351         2814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_50035_length_2814_cov_2.837985, whole genome shotgun sequence.
ACCESSION   DAOPHV010000351 DAOPHV010000000
VERSION     DAOPHV010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50035_length_2814_cov_2.837985"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /gene="rfbD"
                     /locus_tag="PLQ29_09240"
     CDS             <1..764
                     /gene="rfbD"
                     /locus_tag="PLQ29_09240"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPG86869.1"
                     /translation="SILDPAALEAFAAGKGIVCIVNCAAYTAVDKAETEEDLARKLNA
                     DGPENIGRLAANLGARVVHVSTDYVFDGNGKSPYKEGDPVNPASAYGRTKAEGEARLL
                     AACPSAMIVRTAWLYGRYGPNFVATMLRLMAEKDELGVVMDQRGSPTWAYDLAVALAD
                     LAVAEDVPSGPYHFTNDGETSWYDFAVEIQRLGRELGVLSRDCRVKPLTTAEYGSKTR
                     RPAYSVLSKERIKALGVPVPGWKESLRAYFTRDLI"
     gene            839..1231
                     /locus_tag="PLQ29_09245"
     CDS             839..1231
                     /locus_tag="PLQ29_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HPG86870.1"
                     /translation="MAEGMSELSQRVIGCVFTVHNELGPGFLEAVYKNALLYELSCAG
                     LQAEAEAPLSVCYKSQTVGVFYADLIVEHCLICELKAVRRLDVSHEMQLVNYLKATGL
                     NDGLLINFGSARAEIKHKYRLNKELKII"
     gene            1324..2394
                     /gene="rfbB"
                     /locus_tag="PLQ29_09250"
     CDS             1324..2394
                     /gene="rfbB"
                     /locus_tag="PLQ29_09250"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPG86871.1"
                     /translation="MRSFKTILVTGGAGFIGSNLIHYLFEKAGYDGRVVNVDALTYAG
                     NLESLADIEKTHGGKRYFFEQADICDRATVEAIFKKYEVDAVCHLAAESHVDRSILGP
                     EAFIKTNVMGTFTLLDVARNAWKGRDDVLFHHVSTDEVFGSLGDSGYFTETTPYDPRS
                     PYSASKASSDHLVQAYHHTYGMPITLTNCSNNYGPYQFPEKLIPVMVLNMMEGKALPV
                     YGDGKNIRDWLYVEDHNSAVWTVMTKGRSGQTYNIGGENEWENIKLLDRLIELVAEES
                     GTSEMALRSLITYVKDRPGHDRRYAIDCGKLKSELGWKQSVSFDEGLRRTVRWYLDSG
                     EWIAHVRSGEYQNWMRKNYTER"
     gene            complement(2561..>2814)
                     /locus_tag="PLQ29_09255"
     CDS             complement(2561..>2814)
                     /locus_tag="PLQ29_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86872.1"
                     /translation="SLYFIRDSNGVEVDLVLDRRPVPLPIEIKASTTYHPDFRKGLER
                     FDAMIGTSATGYVVYGGDADQVGDSLRLIGYKRLSTILT"
BASE COUNT          635 a          770 c          854 g          555 t
ORIGIN      
        1 tatcgatact cgatccggcg gccctcgagg ccttcgcggc gggcaagggg atagtctgca
       61 tcgttaactg cgccgcgtat acggcggtgg acaaggccga gaccgaggaa gacttggccc
      121 gcaagctgaa cgcagacggg cccgagaaca tcggccgcct ggcggcgaac ctaggcgcga
      181 gggtcgtcca tgtgtcgact gactacgtgt tcgacgggaa cggcaagagc ccgtataagg
      241 aaggcgaccc ggtcaatccc gcgagcgctt acgggcgtac gaaggctgag ggcgaggcta
      301 ggctcctggc cgcttgcccg agcgccatga tcgtgcgtac cgcctggctc tacggtaggt
      361 acggccccaa cttcgtggcg accatgctca ggctgatggc cgagaaggac gaactgggcg
      421 tcgtgatgga ccagaggggg agccccacct gggcctacga cctggccgtc gccctggcgg
      481 acctcgccgt agccgaggac gtaccgtccg ggccgtatca ttttacgaac gacggggaga
      541 ccagctggta cgacttcgcc gtggagatac agcggctcgg cagggagctg ggtgtcctga
      601 gccgcgactg ccgcgtgaag ccgctgacta ccgcggagta cggctccaag acccgccgcc
      661 ccgcgtactc ggtcttgtcc aaggaacgca tcaaggccct cggcgtcccc gtccctggct
      721 ggaaagagag cctcagggcg tactttacac gagaccttat atgaagaatg tctcgtgcag
      781 aggaatagag cggtatggat atttaacagg atgacaggat gggaacagga tgcacaggat
      841 ggcagaggga atgagcgagc tatcacaacg cgtgatcggt tgcgtcttta ctgtccataa
      901 tgaactcggt cctggcttcc tcgaggcagt atataagaac gcattactct atgagttatc
      961 ctgcgccgga ctacaggcag aagccgaggc tcctttaagc gtatgctata agagtcagac
     1021 agtaggggta ttctacgcgg atttgattgt tgagcattgt ttaatctgcg agctcaaggc
     1081 ggtaagaagg ctcgatgtaa gtcatgagat gcagctagtt aactatctaa aggctacggg
     1141 cttgaacgat ggcttactga ttaattttgg atcagccagg gcagagataa aacataagta
     1201 caggttgaac aaagagttaa aaattatata gctttccatt atgtccatcc tgttccttat
     1261 cctggatatc ctgtttagct agttataatg gcgaattgta tcgttctatt aaaaggagta
     1321 tttatgagat cattcaagac tattctggtt accggcgggg cggggttcat cggttcgaac
     1381 ctgatccact acctgttcga gaaggccggc tacgatggcc gcgtcgtcaa cgtcgacgcg
     1441 ctcacgtacg ccggtaacct ggagagcctg gccgatatcg agaagactca tggcggtaag
     1501 cgctacttct tcgagcaggc cgacatctgc gaccgcgcga ccgtcgaggc catcttcaag
     1561 aagtacgagg tagacgcggt ctgccacctg gccgccgaga gccacgtgga ccggtcgatt
     1621 ctcggacccg aggcttttat caagacgaac gtgatgggca cctttaccct cctggacgtg
     1681 gcccggaacg cctggaaggg ccgggacgac gtcctcttcc accacgtgag taccgacgag
     1741 gtcttcggct ccctgggcga ctcgggctac tttaccgaga ccacgcccta cgacccgcgc
     1801 tcgccgtatt ccgccagcaa ggcgtcgagc gaccacctgg tgcaggccta ccaccatacc
     1861 tacggtatgc ccataacctt aaccaactgt tccaacaact acggacccta ccagtttccc
     1921 gagaaactca ttcccgtcat ggtgctgaac atgatggaag gcaaggccct gccggtctac
     1981 ggcgatggta agaacatccg cgactggctc tacgtggagg accataactc cgcggtctgg
     2041 accgtgatga cgaagggccg ttccggccag acttacaaca taggcggtga gaatgagtgg
     2101 gagaacatca agctgctgga ccgcctcatc gagctcgtgg ccgaggaatc gggcacgtcc
     2161 gagatggcgc tacgctcgct gatcacctac gtgaaggacc gcccgggcca cgaccgccgc
     2221 tacgcgatag actgcgggaa gctcaagagc gagctcggct ggaagcagtc ggtcagcttc
     2281 gacgagggcc tacggcggac ggtgcgctgg tacctggact caggcgagtg gatagcccac
     2341 gtgcggtccg gcgagtacca gaactggatg cggaagaact ataccgagcg ataacggaga
     2401 agatgtcgta tagagggaac gcggaacatg atggtacagg attgaagaag atgaacaaga
     2461 ttaagcaaag accaggttta ggacttatcc tgatcatcct gctacatctt gatcatcatg
     2521 ttctgcattc ttagcgatct tcacgtgggg atataggccc ctaggtcagt atcgtcgata
     2581 ggcgcttgta gccgatgagc ctgagcgaat cgccgacctg atccgcgtcg cctccgtata
     2641 ccacgtagcc ggtggcgctc gtccctatca tggcgtcgaa gcgttccagc cccttgcgga
     2701 aatccgggtg gtaggtcgtg gaagccttga tctcgatagg aagcggtacg ggcctacggt
     2761 ccaggaccag gtcgacctcg acgccgttac tgtcgcgtat gaaatagagc gacg
//
LOCUS       DAOPHV010000352         2810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_50108_length_2810_cov_4.692196, whole genome shotgun sequence.
ACCESSION   DAOPHV010000352 DAOPHV010000000
VERSION     DAOPHV010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50108_length_2810_cov_4.692196"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLQ29_09260"
     CDS             complement(<1..239)
                     /locus_tag="PLQ29_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG86873.1"
                     /translation="MAENKNENLLEVSGLKMHFPYHTGSLWTKKKGFIYAVDGVDLAI
                     KKGETLGLVGESGCGKSTTLRAVAQLYRPTAGSVKL"
     gene            complement(239..1222)
                     /locus_tag="PLQ29_09265"
     CDS             complement(239..1222)
                     /locus_tag="PLQ29_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG86874.1"
                     /translation="MTDDVILQVKDLKAYFKLDEGLLKAVDGVSFDLRKGETLGIVGE
                     SGSGKSVTNLAIMRLIPSPPGHIAGGEVVFMGDDILKMSDDGIRKIRGNKISMIFQDP
                     MTCLNPFLKISTQMIETIVLHQGLDKKAAKEKAVEMLKLAGIPAPEKRIDQYPHQFSG
                     GMRQRVMIAMALSCEPEILIADEPTTALDVTIQAQILELINELSERLGTAVILITHSL
                     GVVAGMCDSICVMYAGRIVERGTADEIFSDPRHPYTQGLIKSVPRLDKVSTQRLYSIP
                     GQPPNVINLPDCCPFFPRCERAMDVCKTKYPKVAELGDKRTVACWLYGEGK"
     gene            complement(1236..2321)
                     /locus_tag="PLQ29_09270"
     CDS             complement(1236..2321)
                     /locus_tag="PLQ29_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG86875.1"
                     /translation="MPSIDKNNDRATNEFNQYVKPVSLWADAWKRLRRNKMAVLGMWI
                     VAIYMVVTFGAALLPFKSYTEQEMLHINLPPSFKPAGVVALGRLEERKVELEAAITRS
                     GRADLKQDLTRIENEIIQLKGEVDTDPKHKRVYILGTDYLGRDMLARTIYGGQISIMI
                     GLIGTLTAAMIGIVLGSLGGYLGGWVDNLIGRFVDLMYSLPYMLVVIIIMSMLPTGSN
                     SIFVLYIAIAMISWLTISRVVRGQIISLKNSEFVEAARSMGAGSGRIIFRHLLPNSLG
                     IIIIYSTLQFPSFIMNESFLSFLGLGVSAPRASWGTLVSEGVQGMQLYPWRLLVPAVS
                     MTVFLFAMNFLGDGLRDALDPQSKNRN"
     gene            complement(2335..>2810)
                     /locus_tag="PLQ29_09275"
     CDS             complement(2335..>2810)
                     /locus_tag="PLQ29_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG86876.1"
                     /translation="IFALKLKWLPTSGWLSSRAGALTLIMPIITLMMPYFAYIARMSR
                     GSLIEVMRSDYVRTARAKGLKESVVIRKHVLKGAMLPIVTYLGPAFSGIITGSVVVEQ
                     IFVIPGIGRIFVQSALNRDYTVIMGDVIVYSIILIAANFVVDILYGLLDPRISYK"
BASE COUNT          505 a          836 c          918 g          551 t
ORIGIN      
        1 agcttgacgg agccggccgt cggccggtac agctgcgcga cggcgcggag cgtggtcgac
       61 ttgccgcagc ccgactcgcc gacaagaccc agggtctcgc ccttcttgat cgccaggtcc
      121 acgccgtcga cggcgtagat gaagcccttc ttcttggtcc acagcgagcc cgtgtggtac
      181 gggaagtgca tcttgagtcc ggatacctcg aggaggttct cgttcttgtt ctcggccatt
      241 atttcccctc cccgtagagc cagcaggcca cggtgcgctt gtcgcccagc tcggctacct
      301 tgggatactt ggtcttgcag acgtccatag cgcgctcgca gcgcgggaag aacgggcagc
      361 agtccggcag gttgatcacg ttcggcggct gtcccgggat cgagtacagt cgctgcgtcg
      421 acaccttgtc caggcgcggc acgctcttga tcagaccctg cgtgtacgga tgcctcgggt
      481 cggagaatat ctcgtccgcg gtgccgcgct cgacgatgcg gccggcgtac atgacgcaga
      541 tcgagtcgca cataccggcc accacgccga gcgagtgggt tatcaggatc accgcagtgc
      601 cgaggcgctc ggacagctcg ttgatcagct cgaggatctg agcctggatg gtgacgtcga
      661 gggccgtggt cggctcgtcg gcgatcagga tctccggctc gcaggacagg gccatggcta
      721 tcatcacccg ctggcgcata ccgccggaga actggtgcgg atactggtcg atacgcttct
      781 cgggcgccgg tatgccggcg agcttgagca tctcgacggc tttctccttg gccgccttct
      841 tgtccagccc ctggtgcagc acgatcgtct cgatcatctg ggtgctgatc ttaaggaagg
      901 ggttcaggca ggtcatcggg tcctggaaga tcatcgagat cttgttgccg cggatcttcc
      961 ggatgccatc gtcggacatc ttgaggatgt cgtctcccat gaacacgacc tcgccgccag
     1021 cgatgtgtcc gggcgggctc ggtatcagcc tcatgatggc gaggttggta acgctcttgc
     1081 cggagccgga ttcgccgact atgccgagcg tctcgccctt gcgaaggtcg aagctgacgc
     1141 cgtcgaccgc cttgaggaga ccctcgtcca gcttgaagta cgccttcagg tccttgacct
     1201 ggagtatgac gtcgtcggtc attctagtgg tctccttagt ttcggttctt gctctgcgga
     1261 tccagggcgt cgcgcaggcc gtcgccgagg aagttcatcg cgaagaggaa caccgtcatc
     1321 gagacggccg gcacgagcag gcgccacgga tagagctgca tgccctggac gccctcgctg
     1381 acgagggtac cccacgaggc gcgcggcgcc gagacgccga ggccgaggaa ggacaggaag
     1441 ctctcgttca tgatgaagct cgggaactgc agcgtcgagt agatgatgat gatgccgagc
     1501 gagttcggca ggaggtgcct gaagatgatg cggccggagc cggcgcccat cgacctggcg
     1561 gcctcgacga actcggagtt cttgaggctg atgatctggc cgcgcacgac gcgggagatg
     1621 gtcagccacg atatcatcgc tatggcgatg tacagcacga agatgctgtt cgacccggtc
     1681 ggcaacatcg acatgatgat gatgacgacg agcatgtacg gcaggctgta catcaggtcg
     1741 acgaagcggc ctatcaggtt gtcgacccag ccgcccaggt agccgccgag cgagccgagc
     1801 acgatgccta tcatcgccgc ggtcagcgtg ccgatgaggc cgatcatgat cgatatctga
     1861 cccccgtaga tggtcctggc gagcatatcg cggcccaggt agtcggtacc gagtatgtag
     1921 acgcgcttat gcttgggatc ggtgtcgacc tcgcccttga gctggatgat ctcgttctcg
     1981 atgcgcgtca ggtcctgctt gaggtcggcc cgtccgctcc gcgtgatggc ggcctcgagc
     2041 tctaccttgc gctcttcgag ccgaccgagc gccacgacgc cggccggctt gaaggacggc
     2101 ggcaggttga tgtggagcat ctcctgctcg gtgtagctct tgaacggtag cagggccgcc
     2161 ccgaaggtga cgaccatata gatggcgacg atccacatac cgagtacggc catcttgttg
     2221 cggcgcaggc gtttccacgc gtccgcccac agcgagaccg gcttcacgta ctggttgaat
     2281 tcgttggtag ccctatcgtt gttcttgtcg attgacggca tatccgatgg ctccttattt
     2341 gtacgagatg cgcgggtcga ggaggccgta caggatatcc acgacgaagt tggccgcgat
     2401 caggatgatc gagtagacga tgacgtcgcc catgatcacc gtgtagtcgc ggttcagcgc
     2461 cgactgcacg aagatgcggc cgatgcccgg tatcacgaag atctgctcca cgacgaccga
     2521 tccggtgatg ataccggaga aggccggccc gaggtaggtg acgatcggca gcatcgcgcc
     2581 cttgagcacg tgcttgcgga tgaccacgct ctccttgagg cccttggccc gggcggtccg
     2641 cacgtagtcg gagcgcatca cctcgatcag cgagccgcga gacatcctcg cgatataggc
     2701 gaaatagggc atcatcaggg tgatgatggg catgatcagg gtcagggcgc cggcgcgact
     2761 cgatagccag ccggacgtcg gtagccattt gagcttgagc gcgaagatga
//
LOCUS       DAOPHV010000353         2804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_50250_length_2804_cov_3.396508, whole genome shotgun sequence.
ACCESSION   DAOPHV010000353 DAOPHV010000000
VERSION     DAOPHV010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50250_length_2804_cov_3.396508"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="PLQ29_09280"
     CDS             <1..1439
                     /locus_tag="PLQ29_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86877.1"
                     /translation="LARIDASPATASAEAYLNAAALAKGSEDRLRVLKRAALRDSALY
                     ADVAAVMTGSPVSQPVALAALDAYLKAGRFVEALSLFDGPLDPRTNAAALAETVVTAA
                     RAGRALSPSIDQLVACFDATGDSSFLTSAAIDAMRKGDRQAASMLLRDAELAPYRLLW
                     DAMALEPLARRVPVSEEPLELAVRADAERLLGETVAATASYAFIVTNYPDWSWKPYAS
                     LARLCGAEGASAAPRWPYSAVPGSSESLASPTALAERLHGEMLGRFSDDSDAVIERAS
                     WLYDNDRSAEASSLLVGLEGERASIARLAYGSPDRAVPDAARLAATYPLSAAAVDAAL
                     GAFASAGSWDRFVAALGEASAAELPLPRLWFWEAVYSALNGEPAQAAEAIRRNGGALS
                     DYASAYDIGILELAAGDYRAAADALGAAAAIAGSAEAMAKAYVLLGDALSEQRLTREA
                     RGAYEAALGADPGSRDARSKLYRLDASP"
     gene            1534..2292
                     /gene="map"
                     /locus_tag="PLQ29_09285"
     CDS             1534..2292
                     /gene="map"
                     /locus_tag="PLQ29_09285"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPG86878.1"
                     /translation="MIRLKNEKQMAGIKASCRLLSDMYAELRPRIAAGVTTGELDRFA
                     HAYIVEHGGTPAFLNYGDRSNPFPATLCVSINEEVIHGIPGNRVIRPGDVVGIDSGIE
                     LGGFYSDAAFSVAVPPVAPETEKLLRVTRECLERAIAAVKPGARIHDVSRAVFSHARA
                     NGFGVVRQFCGHGVGFSQHEDPQVPNYVGSGPNPRIVPGMVIAIEPMINAGGDGVAEL
                     DDGWTIVTLDGSASAHFEHTVAVTATGVEVLTAW"
     gene            2403..>2804
                     /locus_tag="PLQ29_09290"
     CDS             2403..>2804
                     /locus_tag="PLQ29_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HPG86879.1"
                     /translation="MSLTRKLFSKKIFLVNLVLVGIMIGFATAFVILARAPSSDSPAV
                     AHAETALPIDATAAVGQAKSIQMAFNEVSKTVLPSVVELDVVGTSTAPQSDDFPWRFF
                     FGDPNQAPDEKTQPKQYEERGLGSGIIVRRQG"
BASE COUNT          451 a          964 c          912 g          477 t
ORIGIN      
        1 tgttggccag gatcgacgcg tcgcccgcga cggcctcggc ggaagcctac ctgaacgccg
       61 ccgccttggc gaagggctcg gaggaccgcc tgcgcgtgct gaagcgggcg gcgctccgcg
      121 atagcgccct ctacgccgat gtggccgcgg tcatgacggg aagcccggtc tcgcagccgg
      181 tggccctggc cgctctcgat gcctacctta aagccggccg tttcgtcgaa gcgctgtcgc
      241 tcttcgacgg tcccctcgat cctaggacca acgccgccgc gctcgccgag accgtcgtca
      301 cggctgcgcg cgccggtcgt gcgctcagcc catcgatcga ccagctggtc gcctgcttcg
      361 acgcgaccgg cgactcgtcg ttcctgacga gcgccgcgat cgacgccatg cggaagggcg
      421 accgtcaagc cgctagcatg ctcctgcgcg acgcggagct cgccccgtat cgccttctat
      481 gggacgcgat ggcgctcgag cccctggctc ggcgggtccc ggtatcggag gagccgctcg
      541 agctggcggt acgagccgac gcggaacggc tcttgggcga aaccgtggcc gccacggcat
      601 cctacgcgtt catcgttacg aattaccccg attggtcatg gaagccgtat gcgtcgctgg
      661 cgcgcctctg tggcgccgag ggcgcctctg cggcgccgcg ctggccgtat tcggctgtgc
      721 ccggctcgtc cgagagccta gccagcccga cggcgctagc cgaacgcctc cacggcgaga
      781 tgctcggccg tttctcggac gatagcgacg ccgttatcga gcgggcttcc tggctgtatg
      841 acaacgacag gtcggcagag gcctcgagcc tcctcgtcgg cctcgagggc gagcgggctt
      901 ccatcgccag gctggcgtac ggctctcccg atcgggccgt gccggacgcc gctcgcctcg
      961 cggcgacata cccgctatcg gctgccgccg tagacgcggc cctcggcgcg ttcgcgagcg
     1021 ccggctcgtg ggatcgcttc gtcgccgctc tgggcgaggc ctcggcggcg gagcttccgt
     1081 taccgaggct ctggttctgg gaggccgtct atagcgcgct gaacggagag ccggcccagg
     1141 ccgcggaggc gatcagacgc aacggaggcg ccttatcgga ctatgcctcg gcctacgata
     1201 tcggtatcct cgagctggcc gccggcgact atcgcgcggc cgccgacgcc ctcggcgctg
     1261 ccgcggctat cgccggttcg gcggaagcta tggccaaggc ttatgtgctg ctcggagacg
     1321 cgctatccga gcagcggctg acacgcgagg cgcgcggcgc ctacgaggcg gctctcgggg
     1381 ccgatcccgg cagccgggac gcccggtcca agctctaccg cctcgacgcc tcgccgtgaa
     1441 ccgccacacc gcgaagcgac gagccaaggc cgctcgcatg attttcgcgc cgcgatgaag
     1501 cggccgggct cccaagccca gaaaggcact ctcgtgatac gcttgaagaa cgaaaagcag
     1561 atggccggca tcaaggcgtc ctgcaggctg ttgtcggata tgtacgccga gctcaggccg
     1621 aggatagcgg ctggcgtaac gaccggcgag ctcgaccgct tcgcccatgc ctatatcgtc
     1681 gaacacggcg gcacaccagc ctttctgaac tacggcgacc gatcgaatcc gttcccggcg
     1741 acgctctgcg tgtccatcaa cgaggaagtt atccacggca ttccaggaaa ccgcgtcatc
     1801 cgtcctggag acgtcgtcgg catcgatagc ggcatcgagc tcggcggctt ctatagcgac
     1861 gcggccttca gcgtcgccgt ccccccggtc gctcccgaga ccgaaaaact gctgcgcgtt
     1921 acccgcgagt gccttgagcg agcgatagcc gcggtcaagc ctggcgccag gatccatgac
     1981 gtctcccgcg ccgtattctc gcacgcccgc gccaacggtt tcggcgtcgt caggcagttc
     2041 tgcggtcacg gcgtcggctt ctcccagcac gaggacccgc aggtcccgaa ctacgtcggg
     2101 tcgggcccca acccgcgtat cgtgccaggc atggtcatcg ccatcgaacc gatgataaat
     2161 gccggcgggg acggcgtggc cgaactcgat gacggctgga cgatcgttac cctcgacggc
     2221 tccgcctcgg cacatttcga gcatacggtc gccgtgacgg cgaccggcgt cgaggttctg
     2281 acggcctggt agccgtggcc cgcgaggagt aaccgaacgg cgacgcaatt agttatcttt
     2341 gcatagtcgg tcgcggcgag ccgacggcca accattcttc taatacgttc ggaggtgtac
     2401 cgatgtcctt aacgaggaaa ctgttctcta agaaaatatt cctggtcaac ctggtgctcg
     2461 tcggcatcat gataggtttc gctaccgcct tcgtcatact ggcgcgggcg ccttcgtccg
     2521 atagcccggc cgttgcgcac gccgagaccg ccctgcccat cgatgcgacg gccgccgtcg
     2581 ggcaggccaa gtcgatacag atggccttca acgaggtctc gaagacggtg ctaccgtcag
     2641 tcgtcgagct cgacgtggtg ggtacctcga ccgcgcccca gagcgacgat ttcccatggc
     2701 gtttcttctt cggcgatccg aatcaggctc ccgatgaaaa gacgcagcct aagcaatacg
     2761 aggagcgcgg cctaggctcg ggtatcatcg tccggaggca aggc
//
LOCUS       DAOPHV010000354         2802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_50297_length_2802_cov_4.398617, whole genome shotgun sequence.
ACCESSION   DAOPHV010000354 DAOPHV010000000
VERSION     DAOPHV010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50297_length_2802_cov_4.398617"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..1218
                     /locus_tag="PLQ29_09295"
     CDS             55..1218
                     /locus_tag="PLQ29_09295"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HPG86880.1"
                     /translation="MAPISTWVVYVMGTYKDIPEFQGLGVGDISFFVKTIPFNLYAIF
                     AVFMVLFITLTHKDFGPMARSEYRADKGIGLYDEEKYGAVSSQVESKAMTSNAKAFDF
                     LIPIAALIAIALFFFPMTTWMGAIDGEEITSLGQAMAAIPFGQAFNDTDSSMALMYAA
                     VFANVFAYIYYIARRLLNIKNATDALMDGFRAMVPAVMILTLAWALGSVIKTSRPDGG
                     VGLAEYVSEIVINGNFPMTMLPLVVYIFSCLISFASGTSWGTMGIMIPIAVPVIIQLA
                     RAAGLPADQTLNATALTIGAVMGGSVFGDHCSPISDTTVLSSTGAAVPHLEHVATQLP
                     YALFVAVCVMIGTVAAGLTWNPIVGLVVTGVLFVAGVYLLPVWFGPKKYEAEK"
     gene            1348..2022
                     /locus_tag="PLQ29_09300"
     CDS             1348..2022
                     /locus_tag="PLQ29_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HPG86881.1"
                     /translation="MIQKPIISAAEAAAKIKPGDVVHVGGFLGCGSPDAIIAAIVSAG
                     VDGLTLVCNDTGIYNEAKGVSNGPAPLVQAKAFKTMIVSHIGTNPETQRQMNSGESEV
                     VLVPQGTLAERIRAAGNGLGGVLTPTGVGTEVEEGKRKLTLKGKDYLLEEALSGDVAI
                     IKAKKADKAGNLVYAKTARNFNPLMATACGTVIVEAEEIVEVGAIDPDQVHTPSIFVD
                     FIVQAE"
     gene            2035..2697
                     /locus_tag="PLQ29_09305"
     CDS             2035..2697
                     /locus_tag="PLQ29_09305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HPG86882.1"
                     /translation="MIEYVENKEIIARRIARFFASGDVVNLGIGLPTMVGNFVPEGVT
                     VILQSENGMMGLGPAPEPGKEDPMLVNAGGMPVSILPGGCFFDSAMSFGIIRGGHVDY
                     TVLGVLEVDQEGNLANYKIPGKMVPGMGGAMDLVAGSKKVIAATTHLEKSGSSKLKHR
                     CSLPLTAAHEVDIVVTDVGYFTVKDGKFRLEEYFAPYTPEWIVEHTGADVVVAPGCKE
                     FK"
BASE COUNT          505 a          942 c          886 g          469 t
ORIGIN      
        1 gcccggagcc aggcgagcca gcgcgcgagc tagccggccg ccccggtggc catcatggcg
       61 cccatatcga cctgggtcgt ctacgtgatg ggcacctata aggacatccc ggagttccag
      121 ggcctcggcg tcggcgacat ctccttcttc gtgaagacta tacccttcaa cctctacgcc
      181 atcttcgcgg tgttcatggt cctgttcatc acgctgacgc acaaggactt cggcccgatg
      241 gcccgttccg agtaccgcgc cgacaagggc ataggcctgt acgacgagga gaagtacggc
      301 gccgtgtcca gccaggtaga gtccaaggcc atgacctcga acgccaaggc cttcgacttc
      361 ctgatcccga tcgccgccct gatcgccatc gccctgttct tcttcccgat gaccacctgg
      421 atgggcgcca tcgacggcga ggagatcacg agcctcggcc aggccatggc cgccatcccg
      481 ttcggccagg ccttcaacga caccgactcg tcgatggccc tcatgtacgc cgcggtcttc
      541 gcgaacgtgt tcgcgtatat ctactacatc gcccgccggc tgctcaacat caagaacgcg
      601 accgacgccc tgatggacgg cttccgcgcg atggtgccgg ccgtgatgat cctgaccctc
      661 gcctgggccc tcggctccgt catcaagacc agccgcccgg acggcggcgt gggcctcgcc
      721 gagtacgtct ccgagatcgt catcaacggc aacttcccga tgacgatgct gcccctcgtc
      781 gtgtatatct tctcctgcct gatctcgttc gcgagcggca cgagctgggg cacgatgggc
      841 atcatgatcc cgatagcggt tcccgtcatc atccagctgg cgcgcgcggc cggcctcccc
      901 gccgaccaga cgctcaacgc cacggcgctg accatcggcg cggtgatggg cggctccgta
      961 ttcggcgacc actgctcccc gatctccgac acgacggtgc tgtcgtcgac cggcgccgcg
     1021 gtgccgcacc tcgagcacgt ggccacgcag ctaccctacg cgctgttcgt cgccgtctgc
     1081 gtgatgatag gcacggtggc cgcgggcctg acctggaacc cgatcgtcgg cctggtcgtg
     1141 accggcgtcc tgttcgtcgc cggcgtctac ctgctgccgg tctggttcgg ccccaagaag
     1201 tacgaagcgg aaaagtaacc gtaacggaat aatcctaagg ggccggccgc gctagcgagc
     1261 ccggcccctt ttctatccgg ctccatggta tactcgtcgt cgccatgccg gcgccgaggc
     1321 gcccggaggc gaatctcgga ggttccaatg atacagaaac ccatcatcag cgccgcggaa
     1381 gccgcggcca agatcaagcc cggcgacgtc gtgcacgtcg gcggcttcct gggctgcggt
     1441 agtcccgacg ccataatcgc ggccatcgtc tcggccggcg tcgacggcct gacgctcgtc
     1501 tgcaacgaca cgggcatcta caacgaggcc aagggcgtct cgaacggccc ggccccgctg
     1561 gtccaggcca aggccttcaa gaccatgatc gtctcccaca tcggcaccaa tcccgagacg
     1621 cagcgccaga tgaattcggg cgagtcagag gtcgtgctgg tgccgcaggg taccctggcc
     1681 gagcggatcc gagcggccgg caacggcctc ggaggcgtgc tgacgccgac tggcgtcggc
     1741 accgaggtgg aggagggcaa gcggaagctg acccttaagg gcaaggacta cctgctcgag
     1801 gaggcgctct ccggcgacgt ggccatcatc aaggccaaga aggccgacaa ggccggaaat
     1861 ctcgtctacg ccaagacggc ccgcaacttc aacccgctga tggccacggc ctgcgggacg
     1921 gtcatcgtcg aggccgagga gatcgtcgag gtcggcgcga tagacccgga ccaggtgcac
     1981 acgccgagca tcttcgtcga cttcatcgtc caggccgagt aagggagcgc cgccatgatc
     2041 gagtacgtag agaacaagga aatcatcgcg cgcaggattg cgcgcttctt cgcgtcgggg
     2101 gacgtcgtca acctcggcat cggcctaccg acgatggtcg gcaacttcgt gcccgagggc
     2161 gtgaccgtga tcctgcagtc ggagaacggc atgatgggcc tcggccccgc gccggagccg
     2221 ggtaaggaag acccgatgct cgtcaacgcc ggcggcatgc cggtgagcat cctgcccggc
     2281 ggctgcttct tcgactcggc gatgagcttc ggcatcatcc gcggcgggca cgtcgactat
     2341 accgtactgg gcgtgctcga agtcgaccag gagggcaacc tggccaacta caagataccc
     2401 ggcaagatgg tccccggcat gggaggcgcc atggatctcg tcgcgggctc caagaaggtc
     2461 atagcggcga cgacccacct cgagaagtcg ggctcgtcga agcttaagca tcgctgctcc
     2521 ctgcccttga cggccgccca cgaggtggac atcgtcgtga ccgacgtcgg ttacttcacg
     2581 gtcaaggacg ggaagttccg tctggaggaa tacttcgctc cgtacacgcc ggagtggata
     2641 gtcgagcata ccggcgccga cgtcgtcgtg gccccgggct gcaaggaatt caagtaagca
     2701 tgacgggccg cccgcgcgac gggcggcctt ttcatttcat gttcctgggg gggaatcaat
     2761 gaaagacgca ttcatcgtag gcgccgcgag gacggcggta gg
//
LOCUS       DAOPHV010000355         2800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_50350_length_2800_cov_3.942441, whole genome shotgun sequence.
ACCESSION   DAOPHV010000355 DAOPHV010000000
VERSION     DAOPHV010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50350_length_2800_cov_3.942441"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLQ29_09310"
     CDS             complement(<1..495)
                     /locus_tag="PLQ29_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPG86883.1"
                     /translation="MGRPFNPGYILEVLPRLLAFLPVTLGVAAATAVLGTMLGFVLAK
                     AKLGSSALARGLADAYTWVLRCTPSIVLLFLVYYGLPALVKAAFGVDASRWHRAFYVV
                     LTFTLFFAATMSELMRSAYLAVDRGQREAALSHGLSEFQAFRRIVLPQAAVSALPHVA
                     TALVA"
     gene            complement(503..1363)
                     /locus_tag="PLQ29_09315"
     CDS             complement(503..1363)
                     /locus_tag="PLQ29_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPG86884.1"
                     /translation="MKSIHDSASLGLAALVAAAALALVSSCDRPRGDQAADEAPRVVY
                     SAHTQNYVPYGYIDEKGGSDGFEIAVLRAVDELLPDYEFRFVPTSDEDLFIGLETGRY
                     QLATKGAWATAERREKFVFPKNPIAASVIGIAFRSEDSGRIKDLDSFAAYSGKLVPIS
                     PQSAQYTVVSDYNAAHPEHPIKLVPSDVFIINDAYLWVLEGRYDAFFDIKLSFQKNVE
                     AEDGPFHHLASRLAYAPYKGIPTWPLFARGEQALADAYDGAIERLKAEGTLAELSRRY
                     FGEDVFRFAE"
     gene            complement(1461..>2800)
                     /gene="ftsH"
                     /locus_tag="PLQ29_09320"
     CDS             complement(1461..>2800)
                     /gene="ftsH"
                     /locus_tag="PLQ29_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPG86885.1"
                     /translation="EELVEVVDFLKNPKKYTDIGGKIPKGVLLVGPPGTGKTLLARAV
                     AGEAGVPFFRMSGAEFVEMFVGVGAARVRDLFKQAREKAPCIVFIDELDAIGKSRVNG
                     VMGGNDEREQTLNQLLVEMDGFDATSGLIILAATNRPDVLDPALLRPGRFDRQVGVDR
                     PDIVGREAILDIHSKAVKLDPSVDIKKVARATPGFVGADLANVVNEAALLAVRAGRKK
                     VHQADFEKAVEKIMTGLEKKSRVMNPDERRVIAYHEAGHAIVAAFTPEADPVQKVSIV
                     PRGFGALGFTLQVPIEDRYIVTEDKLLGEIDVLMGGRAAEELTFGKVSTGAANDITRA
                     TDIARRMITDYGMSERYRNVALTKRGAGMMGPSAEPVMAREYGEETQRYIDETVAATV
                     AARYEIARRTLDERRGLLDTLAGELLEKESIDEAAFKAIVAPPRASDERLVSS"
BASE COUNT          444 a          909 c          950 g          497 t
ORIGIN      
        1 ggcgaccagc gccgtggcga cgtgcggcag ggccgatacg gccgcctgag gcagcacgat
       61 gcgacggaag gcctgaaact cgctcaggcc gtggctcagg gccgcctctc gctgaccacg
      121 gtcgaccgcg aggtaggccg agcgcatcag ctcggacatc gtcgccgcga agaacagcgt
      181 aaaggtgagc acgacgtaga aggcgcggtg ccaccggctg gcgtcgacgc cgaaggccgc
      241 cttaacgagg gccggtaggc cgtagtagac gaggaacagg agcacgatcg acggcgtgca
      301 acgcaggacc caggtgtacg cgtcggccag acccctggcc agggccgagc ttccgagctt
      361 ggccttggcc aacacgaagc cgagcatcgt cccgagtacc gccgtggccg ccgccacgcc
      421 tagcgtcacc ggcaagaagg cgagcagccg aggcagtacc tcgaggatgt atcccgggtt
      481 gaacgggcgt cccacggtgc gcctactccg cgaagcggaa gacgtcctcg ccgaagtaac
      541 gccttgacag ctccgccagc gtgccctcgg ccttgagtct ctcgatggcc ccgtcgtagg
      601 cgtcggccag ggcttgctcg cccctggcga acagcggcca ggtcgggatg cccttatacg
      661 gcgcataggc tagccgcgag gccaggtggt ggaaggggcc gtcttcggcc tcgacgttct
      721 tctggaacga tagcttgatg tcgaagaagg cgtcgtagcg gccctcgagc acccacaggt
      781 atgcgtcgtt gatgatgaac acatccgacg gcacgagctt gatcggatgc tccggatggg
      841 ccgcgttata gtcggaaacg acggtatact gggcgctctg cggcgagatc ggcacgagct
      901 tacccgagta ggcggcgaag ctgtccaggt ccttgatccg tcctgagtcc tcgctcctga
      961 aggctatgcc gatgacgctc gcggctatgg ggttcttcgg gaagacgaac ttctcgcggc
     1021 gctccgcggt cgcccacgcg cccttggtgg cgagctggta gcggcccgtc tcgaggccaa
     1081 tgaacaggtc ctcgtccgag gtcggcacga agcggaattc gtagtcgggc aggagctcgt
     1141 cgactgccct gagtacggcg atctcgaagc cgtcggaacc gcccttctcg tcgatgtaac
     1201 cgtagggcac atagttctgg gtgtgggccg aatagacgac gcgcggggcc tcgtccgcgg
     1261 cctggtcgcc tcgcggcctg tcgcaggacg agacgagggc cagggccgcc gcggcgacga
     1321 gcgccgctaa gcctagcgac gcgctatcgt ggatcgattt catgggtatc ctccagtatg
     1381 cggcccgatc ggctacatcg ggcacgcatg cacactgaag tgtatcgcaa actgtattgt
     1441 ttgtctagcc gatagaaacc ctaggagcta accagcctct cgtcgctcgc ccggggcggc
     1501 gcgacgatgg ccttgaaggc cgcctcgtcg atcgactcct tctcgagtag ctccccggcc
     1561 agcgtgtcca ggaggccgcg gcgctcgtcg agggtgcgcc gagcgatctc gtagcgcgcg
     1621 gcgaccgtcg cggcgaccgt ctcgtcgatg tagcgctgcg tctcctcgcc gtactctctg
     1681 gccatcaccg gctcggccga cggccccatc atgcctgcgc cgcgcttggt tagcgccacg
     1741 ttgcggtagc gttccgacat gccgtagtcg gttatcatgc ggcgagctat gtcggtcgcc
     1801 ctggtgatgt cgttcgcagc gcccgtcgac accttgccga aggtcagttc ctcggcggcg
     1861 cggccgccca tcaggacgtc gatctcgccc aggagcttgt cctccgtgac gatataccgg
     1921 tcctcgatcg gtacctgcag cgtgaagccg agggcgccga agcctctagg cacgatggac
     1981 actttctgca cggggtcggc ctccggggtg aacgcggcga cgatggcgtg gccggcctcg
     2041 tggtaggcta tgacgcggcg ctcgtcgggg ttcatcacgc ggctcttctt ctcgaggccg
     2101 gtcatgatct tctcgacggc tttctcgaag tcagcctggt gtaccttttt ccggccggcg
     2161 cgcacggcga gcagggccgc ctcgttgacg acgttggcca ggtccgcccc gacgaagccg
     2221 ggcgtggccc tcgcgacctt cttgatatcg acgctcgggt cgagcttgac cgccttggag
     2281 tgtatgtcga ggatggcttc gcggccgacg atgtccggcc gatcgacgcc cacctggcgg
     2341 tcgaagcgcc cggggcggag cagggccggg tcgagcacgt cgggccggtt cgtcgccgcc
     2401 aggatgatca ggccgctcgt ggcgtcgaag ccgtccatct cgacgaggag ctggttgagg
     2461 gtctgctcgc gctcgtcatt gccgcccatc acgccgttga cgcggctctt gcctatggcg
     2521 tccagctcgt cgatgaagac gatgcagggg gccttctcgc gggcctgctt gaagaggtct
     2581 cgcacgcggg ccgcgcctac gcctacgaac atctcgacga attccgcgcc gctcatgcgg
     2641 aagaacggta cgcctgcctc cccggcgaca gctcgcgcga gcagggtctt gccggtgccc
     2701 ggggggccga cgagcagcac gcccttgggt attttgccgc ctatgtcggt gtacttcttg
     2761 ggattcttga ggaagtcgac gacctcgacc agctcctcct
//
LOCUS       DAOPHV010000356         2793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_50533_length_2793_cov_3.738130, whole genome shotgun sequence.
ACCESSION   DAOPHV010000356 DAOPHV010000000
VERSION     DAOPHV010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50533_length_2793_cov_3.738130"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..154
                     /locus_tag="PLQ29_09325"
     CDS             17..154
                     /locus_tag="PLQ29_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86886.1"
                     /translation="MKPRSIRTPIPGVYQAGQWTFSPSGLPISILTGKLAADAVVKDL
                     R"
     gene            complement(186..662)
                     /locus_tag="PLQ29_09330"
     CDS             complement(186..662)
                     /locus_tag="PLQ29_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPG86887.1"
                     /translation="MQDCEYSGTETGRVPSPLTPETVRELWSRTYNTAGKPDWSHTFP
                     YYHPDIVFQDSIQRVEGIEAFKALCGRLTDRCEELRMDIHSVASAGDSIHMQWTMTMM
                     FKKFPSTPIYGATVLTLHEDGRIIGQRDYYDLWGDIFNGIPWFKGPYRRFMARKFG"
     gene            complement(771..1391)
                     /locus_tag="PLQ29_09335"
     CDS             complement(771..1391)
                     /locus_tag="PLQ29_09335"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPG86888.1"
                     /translation="MKKTLRIITQLAFLALFALLFAKGRIQLWLAVFGSGAILSLVFS
                     RFYCGWICPMNTLFRPLGWLYGKLGVKRLKTPGFMKRPAFRYVFFVLFVGTMLVTKKL
                     GMELPLLAILTGIAVFVTLFFEEALWHNHVCPYGTILSLTSRKAVKSEKIDEAACVSC
                     GKCRTVCPVDCIDIVADTKKMRIRTPDCLTCHACEAVCPTGAISYR"
     gene            complement(1410..2666)
                     /locus_tag="PLQ29_09340"
     CDS             complement(1410..2666)
                     /locus_tag="PLQ29_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86889.1"
                     /translation="MNTNHRILPASALAFVMFFSVAVTGMAFADDGVELSGYGRAKAG
                     ALLSDGSLFLAETTLDAKLSWETGDSTLLVDVAVSERLGGSPELELRELYLRYSGDAV
                     ELVIGKQQIIWGKSDGVFITDIVSPKDLSRFLVPDFVELRQAVDGVRLGGYAGGHSLE
                     LVWLPLFSPGVAPAAGTIWAPVMPYPVTPTITAAALPELSIENGEYFIRYSFMGNAVD
                     LSLMGGWFWNDLPSFAVTGKTMTPGVGITGITLQGEYYRTAAFGYGAAGAIGPLVLRS
                     EGAMQLGKRYQGDFTVYPAGYAEKNSVQYLVGTDFSILGTTFGLQFIQDIILEHDDAL
                     VNERFTNTATVMAVRSFAHETLRVEALAYIGLDVPDALLKAQLVWKMTDAMELSGGAW
                     LFFGDSGQFGQYDGNDSVFAGAKISF"
BASE COUNT          531 a          870 c          880 g          512 t
ORIGIN      
        1 aaggggctga caatcaatga agccgcgatc catacgcacg ccgatacctg gcgtgtacca
       61 ggccggccaa tggaccttca gcccgtcggg gctgccgata tcgatcctga ccggcaagct
      121 cgcggccgac gctgtcgtca aagacctacg ctaggccgcg gcccagccgc gggcccgccg
      181 caggcctagc cgaatttcct ggccataaac cggcggtacg gccccttgaa ccacggtatg
      241 ccgttgaaga tatcgcccca gaggtcgtag tagtcgcgct ggcctatgat gcggccgtcc
      301 tcgtgcaggg tcagcaccgt ggcgccgtag atgggcgtgc tcgggaactt cttgaacatc
      361 atcgtcatgg tccactgcat gtgtatcgag tcgcccgcgc tggccaccga gtggatatcc
      421 atgcgcagct cctcgcaacg gtccgtgagc ctcccgcaga gcgccttgaa cgcctcgatg
      481 ccctcgaccc gctggatcga gtcctggaag acgatatcag gatgatagta cgggaaggta
      541 tgcgaccaat ccggcttgcc cgcggtgttg taggtcctcg accatagttc cctgaccgtc
      601 tctggcgtca ggggcgacgg gacgcgaccg gtttcggtac ccgaatattc acagtcttgc
      661 atagtatcca cggtaacacg agaggggccc ggcggcaagc tcgcccgccc ttccgcgcag
      721 gatctttaac gccggccgcg ctgttcgtgt agccggcgtc ggccgattcc ctatcggtac
      781 gaaatagccc ccgtagggca tacggcctcg caggcatggc aggtcaggca gtcgggcgtg
      841 cgtatgcgca tcttcttcgt gtccgcgacg atatcgatgc agtcgacggg gcataccgtg
      901 cggcacttgc cgcaggagac gcaggccgcc tcgtcgatct tctcgctctt gacggctttg
      961 cgtgacgtca ggctgaggat tgtgccgtac gggcacacgt ggttatgcca cagggcctcc
     1021 tcgaagaaca gcgtcacgaa aacggctatc ccggtcagga tggccaggag cggtaactcc
     1081 atgccaagct tcttcgtgac gagcatggtg ccaacgaaca gcacgaagaa tacgtaccgg
     1141 aacgctgggc gcttcatgaa acctggcgtc ttgaggcgct tgacgccgag tttgccgtag
     1201 agccatccca gcgggcggaa cagggtgttc atcgggcaga tccagccgca gtagaagcgg
     1261 ctgaacacca gcgacagaat cgcgcccgag ccgaagaccg cgagccatag ctgtatcctg
     1321 cctttggcga acaacagggc gaagagggcc agaaacgcga gctgggttat gatgcgtaac
     1381 gtctttttca tcacgtcagt tccttcttcc tagaatgata tcttggcgcc cgcgaagacg
     1441 ctgtcattgc cgtcatactg gccgaactgg ccagagtctc cgaagaacag ccaggcgccg
     1501 ccggacagct ccatggcgtc cgtcatcttc cacaccagct gggccttgag cagcgcgtcg
     1561 ggaacgtcga gccctatgta cgccagcgct tcgaccctga gcgtttcatg ggcaaagctg
     1621 cggacggcca tcacggtggc ggtgttcgtg aagcgctcgt tgaccagggc gtcatcatgc
     1681 tcaaggatga tgtcctggat gaactgcagg ccgaaggtcg tgccgaggat ggagaaatcc
     1741 gttccgacga ggtattgtac ggagtttttc tcggcatatc cagccgggta gacggtaaaa
     1801 tcgccctggt agcgcttgcc aagctgcatc gcgccctcgg agcgcaggac cagaggtccg
     1861 atggcgccgg ccgcgccgta cccgaaggcg gccgtgcggt agtactcgcc ctggagcgtt
     1921 attccggtta tgccgacgcc aggcgtcatg gtcttgccgg ttacggcgaa cgaaggcagg
     1981 tcgttccaga accagccgcc catcaggctc aggtccacgg cgttgcccat gaacgagtat
     2041 cggatgaaat actcgccgtt ctcgatcgac agctccggaa gcgccgcggc cgttatggtc
     2101 ggggtgacgg ggtacggcat gacgggcgcc cagatcgtac cggcggccgg ggccacaccg
     2161 ggcgagaaca gcggcagcca gaccagttcc aggctgtggc cgcccgcgta gccgccgagg
     2221 cgcacgccgt cgaccgcctg cctgagctcc acgaaatcag gcaccaggaa gcgggacagg
     2281 tccttgggag acacgatgtc ggttatgaac acgccgtcgc tcttgcccca gatgatctgc
     2341 tgcttgccga tgaccagttc gacggcatcg ccggaatacc gcaggtacag ttcgcgcagt
     2401 tcaagctcag ggctgcctcc cagtcgctcg ctgaccgcca cgtcgacgag gagcgtactg
     2461 tcgcccgttt cccaggacag cttcgcgtcc agggtcgtct cggcaaggaa gagcgagccg
     2521 tcggacagga gggcgccggc cttggcgcgg ccatacccgc tcagctcgac gccgtcatcg
     2581 gcgaaggcca tgccggtcac ggcgacgctg aaaaacatca cgaaggccag ggccgacgct
     2641 ggcagaatcc ggtggttcgt attcatcaca gtcccttcat catggcgcgt tccgagaatt
     2701 gctcgtcggc gattccggta tccagctcta tcgcggtgaa ctccatacgg gtccggtggt
     2761 tggactgcgc gttgcgcatc tccgttcggg tta
//
LOCUS       DAOPHV010000357         2788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_50661_length_2788_cov_3.239297, whole genome shotgun sequence.
ACCESSION   DAOPHV010000357 DAOPHV010000000
VERSION     DAOPHV010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50661_length_2788_cov_3.239297"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PLQ29_09345"
     CDS             complement(<1..833)
                     /locus_tag="PLQ29_09345"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPG86890.1"
                     /translation="MNRRVEPTPGETTEPTRLARRLSPVDVMTVAFIFTNIAFIASAR
                     TAIEEPLRLIGGYLGCLAIASAVIAIGGPERYAQPRGGLARALRWFQGLFRRGYPLTL
                     FLYFFVEVTRFDTALFKENLDPFFASLDERLFGFVPSWTLMIEHPSLFLSELLSGAYV
                     LYYASIGVLAIWLYVKDRPAMAEYVFVVMLLVYAACLTYIVLPVVGGRYDPTIRELTE
                     TYRYGPFTRLMSFIYRRSAHEGAAFPSTHATVSLVVALFSLRRARPLAPLFCVNAALI
                     LV"
     gene            complement(830..1516)
                     /locus_tag="PLQ29_09350"
     CDS             complement(830..1516)
                     /locus_tag="PLQ29_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86891.1"
                     /translation="MRRLYIAALSILISLTGCASGPETVSAGLPETVRAFIDDNGWDL
                     GAYADSDLSDLSAYYRHERVYLVDAAMDELWDLYANIDPRLAWRTDKTRFGVVWDPIR
                     EFMYREADDGVPPFAPGQVLVLELRLAGFYRLPVAFRIREIDRASGVIEFVYLEANKS
                     NGKQRLEFAPAIGPDGERRSTIRHSTWYRSGDEFRDERLYGGFHASIIDAFHRAVVES
                     AGYSIAVVES"
     gene            complement(1513..>2788)
                     /locus_tag="PLQ29_09355"
     CDS             complement(1513..>2788)
                     /locus_tag="PLQ29_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HPG86892.1"
                     /translation="DRFFVTRPLDYDASSVLRPRTHPLITPMTLDDGRTVGLWTTFSA
                     DQVDLEFSEPAVFAEFIDIILGYAERGARMLRLDAIAYLWKRDGTSCAHLPETHAVVR
                     AVRAVVDGLGLGMAVLTETNVPHEENLSYFGGFPLIGSGSGGDDSGDEAHMVYNFSLP
                     PLVLHAFATGDAGPLSSWAASLRVPPGGVMLNFLASHDGVGVTPARGLVDDLGPLVRA
                     VRERGGLVSYKATQDGPAPYELNVSWADAVAAPGADESERAAALVASYAIACAMDGVP
                     AVYFHSAIGSRNWTGGPALLGHNRAINRERPRLDELEAALDSPASTRALALAGLSSLL
                     RERALRPAFSPTSPRRGYPAQGPVFIVERGTADEATLAVVNCSRREAPVALPSGWAGA
                     EAFDPVAGVRVPIPAAAAVSLKGYSVLWLHRR"
BASE COUNT          494 a          890 c          989 g          415 t
ORIGIN      
        1 acaaggatca gcgcggcgtt gacgcagaat agcggcgcca aggggcgggc ccgcctgagc
       61 gagaacagcg cgacgaccag gctgacggtg gcatgcgtgc tcgggaaggc ggcgccctcg
      121 tgggcgctac ggcgataaat gaagctcatc agcctcgtga aggggccgta gcggtaggtc
      181 tcggtcagct ctcgtatggt cgggtcgtac cgccccccga cgacgggcag gacgatgtag
      241 gtcaggcagg ccgcgtagac gagtagcatg acgacgaaga catattcggc catcgcgggg
      301 cgatccttga cgtacagcca gatcgccagt acgcctatcg acgcgtagta cagcacgtag
      361 gccccgctca gcagctcgga gaggaagagc gacggatgct cgatcatcag ggtccagctc
      421 ggcacgaagc cgaacaagcg ctcgtcgagc gacgcgaaga acgggtcgag gttctccttg
      481 aacagggcgg tatcgaagcg ggtcacctcg acgaagaagt ataggaacag cgtcagcgga
      541 tagccccgtc ggaacaggcc ctggaaccat cgcagcgccc gggccagccc gccgcgcggt
      601 tgcgcgtagc gttccggccc tccgatcgct atcacggcac tggcgatggc gaggcagccg
      661 aggtagccgc cgatcaaccg caagggttcc tcgatcgcgg tcctcgcgga tgcgatgaac
      721 gcgatattgg tgaagatgaa ggcaacggtc atgacgtcga ccggagacag ccgacgcgct
      781 agccgcgtcg gctccgtcgt ctctcccgga gtaggctcga cgcgtcggtt catgattcga
      841 ccacggcgat ggagtagccg gccgattcga cgacggcccg gtggaacgcg tcgatgatcg
      901 acgcgtggaa cccgccgtac aggcgctcgt ccctgaactc gtcgccggaa cggtaccagg
      961 tgctatggcg tatggtcgag cgccgctcgc cgtcagggcc tatcgccggc gcgaattcga
     1021 ggcgctgctt gccgttcgac ttgttcgcct cgaggtagac gaattcgatc acgcccgagg
     1081 cgcggtcgat ctcccgtatc ctgaaggcga ccggcaggcg atagaatccg gccagcctga
     1141 gctcgagcac cagcacctgg cccggggcga acggcggcac gccgtcgtca gcctcgcggt
     1201 acatgaactc gcgtatcggg tcccatacca ccccgaaacg ggtcttatcg gtacgccacg
     1261 ccaaacgcgg atcgatgttt gcgtacaggt cccatagctc gtccatcgcc gcgtcgacga
     1321 ggtagacccg ttcgtgccgg taataggccg atagatcgga caggtcgctg tcggcgtagg
     1381 ctcccaggtc ccagccgttg tcgtcgatga aggcccggac ggtctcggga aggcccgccg
     1441 aaacggtttc gggcccgctc gcgcatccgg tcagggatat tagtatcgat agcgcggcga
     1501 tatataatcg cctcatcgtc gatgtagcca tagtacgctg tagcctttca gtgatacggc
     1561 cgcggcggcc gggatcggta cgcgtacgcc cgctaccggg tcgaaggctt ccgcacccgc
     1621 ccaacccgaa gggagcgcga cgggcgcttc gcgacgcgag cagtttacca cggcgagcgt
     1681 cgcctcgtca gcggtcccgc gctcgacgat gaagaccggt ccctgggccg ggtagcctcg
     1741 gcgcggcgac gtcggcgaga aggcgggacg gagggcgcgc tcccgcagga gcgacgatag
     1801 gccggccagg gccagggcgc gcgtcgacgc cggcgaatcg agggccgcct cgagctcgtc
     1861 gagcctcggc cgctcgcggt tgatggcgcg gttgtggccg aggagggccg ggccgcccgt
     1921 ccagttgcgc gagcctatcg ccgaatggaa gtagacggcc ggcacgccgt ccatcgcgca
     1981 ggctatcgcg tagctggcga cgagggccgc cgcgcgttcg ctctcatccg cgcccggcgc
     2041 ggctacggcg tcggcccacg atacgttcag ctcgtacggc gccgggccgt cctgggtagc
     2101 cttgtaggag acgaggccgc cgcgttccct gaccgcccgg acgagcggcc cgaggtcgtc
     2161 gacgaggccg cgggccgggg taacgccgac gccgtcgtgg gacgccagga agttgagcat
     2221 gacgccgccg ggcggtaccc gaagcgacgc ggcccagctc gacagggggc cggcgtcgcc
     2281 ggtggcgaaa gcatgcagca cgaggggcgg caggctgaag ttgtagacca tgtgcgcctc
     2341 gtcgccgctg tcgtcgccgc cggatcccga gccgatgagg gggaagccgc cgaagtacga
     2401 caggttctcc tcgtgcggca cgttcgtctc ggtcagcacg gccatgccga gcccgagccc
     2461 gtcgacgacg gcccggacgg cgcgcaccac cgcgtgggtc tcgggcagat gggcgcagct
     2521 ggtaccgtca cgcttccata aataagcgat ggcgtcgagc ctgagcatac gggcgccccg
     2581 ctcggcgtag cccaggataa tatcgatgaa ttcggcgaat acggccggct ccgagaattc
     2641 gaggtcgacc tgatcggcgc tgaaggtggt ccatagcccg acggtacgcc cgtcgtcgag
     2701 ggtcatcggg gtgatcagcg ggtgggtgcg cggacgcagc acgctcgagg cgtcgtaatc
     2761 gagcggcctc gtgacgaaga agcggtcg
//
LOCUS       DAOPHV010000358         2784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_50764_length_2784_cov_4.126420, whole genome shotgun sequence.
ACCESSION   DAOPHV010000358 DAOPHV010000000
VERSION     DAOPHV010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50764_length_2784_cov_4.126420"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /gene="pxpB"
                     /locus_tag="PLQ29_09360"
     CDS             complement(<1..795)
                     /gene="pxpB"
                     /locus_tag="PLQ29_09360"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HPG86893.1"
                     /translation="MPNLSQFECIPLGDDGLLVRLGDVLSEELNERARSLADAVEAAK
                     ADDPADDGIIEASPAYTSVFIDFDPLLLTHAQAEAFVRASLSAVSSGEARRVVRVPVA
                     YGGEAGPDLASIAAEAGLSADEVASIHCSRDYPVYMLGFAPGFPYLGGVDERIASGRL
                     KTPRPRIPAGSVGIAGRQTGVYPFDSPGGWKLVGRSPIRLFDASMDPPATLRPGDLVR
                     FYPVSLDEFDAMSRGPGGIAEHREGPSGPTDPLERAAIRVVRPGLST"
     gene            complement(788..1558)
                     /locus_tag="PLQ29_09365"
     CDS             complement(788..1558)
                     /locus_tag="PLQ29_09365"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002344913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HPG86894.1"
                     /translation="MPRIDLNCDLGEGFGAYSIGDDEGVLRYVTSANIACGFHAGDPS
                     VMERTVALAASAGVSIGAHPGFPDLLGFGRRELSASADEARAMTLYQIGALGAFAKAR
                     GVALTHVKPHGALYNMAAKRADLAAAIAEAVAAYDPALILVGLCGSELLKAGTAAGLR
                     CAGEAFADRAYRADGSLVPRGIPGAVIKDPASCAARAISMATDGFVTALSGERVMLRP
                     DTICLHGDGDGALELAEALSTALREAGVELRPLSHTDA"
     gene            complement(1551..2483)
                     /locus_tag="PLQ29_09370"
     CDS             complement(1551..2483)
                     /locus_tag="PLQ29_09370"
                     /inference="COORDINATES: protein motif:HMM:NF025694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4392 domain-containing protein"
                     /protein_id="HPG86895.1"
                     /translation="MSVSTYEAAGPASIEAAMAFDPGGRGLCGDPRLRVALRGSIRRA
                     ASRLAGADRVLIVTGFLIERAGVGESDGPPGSAVLARALALLGKDVRIVTDLHSAALV
                     EASAAALGSRPRVVTLSPGDGELACLAILSSFRPDVVVAIERPGKAADGGFYSMRGSD
                     MRALVPDTDALVSRASDYGGVSVAIGDGGNELGLGSIGAIIDERVPLGSTIRAVTPAD
                     APIVAGVSNWGAYGLVRALELLALEGGRTISLLHGPGDETAMLEAVAAAGGVDGCSGR
                     REPTVDGLGPESNLAALEAIRAASSIEGEETIDA"
     gene            complement(2512..>2784)
                     /locus_tag="PLQ29_09375"
     CDS             complement(2512..>2784)
                     /locus_tag="PLQ29_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPG86896.1"
                     /translation="PGSCYSQAIPLAAALSVLYMGMTIEETLTALTLNAAAALSLAGD
                     RGSIEAGKLADLLVLDAPSYRHLAYHVGMNVVGTVVKRGKVVSVRT"
BASE COUNT          452 a          939 c          995 g          398 t
ORIGIN      
        1 ggtcgagaga cccggcctga cgacgcgtat ggccgcgcgc tcaagagggt cggtcgggcc
       61 gctcggacct tcgcgatgct cggctatgcc gccaggtccg cggctcatgg cgtcgaactc
      121 gtcgaggctg acgggataga agcgtaccaa gtcgcccggt cttaaggtgg ccggcgggtc
      181 catcgacgcg tcgaagaggc gtatcgggct acggccgacg agtttccacc cgcccgggct
      241 gtcgaaggga tagaccccgg tctgacgccc ggctattccg acggatcccg ccggtattct
      301 ggggcggggc gtcttgagac ggccagaggc tatgcgctcg tcgacccctc cgaggtaggg
      361 gaagcctggc gcgaagccca gcatgtacac cggatagtcc ctgctgcagt ggatggacgc
      421 gacctcgtcg gccgataggc ccgcttcagc ggctatcgag gccaggtcag ggccggcttc
      481 gccgccgtag gccacaggta cccggacgac ccttcgagcc tcgccgcttg agacggcgga
      541 caacgacgcc cggacgaagg cctcggcctg cgcgtgggtc aggaggagcg ggtcgaagtc
      601 gatgaacacc gacgtatagg cgggagacgc ctcgatgatg ccgtcgtcgg ccgggtcgtc
      661 ggccttggcg gcctctacgg cgtccgcgag cgaacgcgcc cgctcgttca gctcctcgga
      721 cagcacgtcc ccgagcctga cgagcaatcc gtcgtcgccg agagggatgc attcaaactg
      781 agataggtta ggcatcggtg tgcgaaaggg gccggagctc gacgccggcc tccctgaggg
      841 ccgtggatag ggcctcggcc agctcgagcg ccccgtcgcc gtcgccgtgg aggcagatcg
      901 tgtcggggcg cagcatgacc cgctcgccgc tcaaggcggt cacgaagcca tcggtggcca
      961 tgctgatggc cctcgcggcg caggaggccg gatccttgat gacggcgccc gggataccgc
     1021 ggggtacgag tgaaccgtcg gcacgatagg ctcggtcggc gaaggcctcg ccggcgcaac
     1081 ggaggcccgc ggcggttccg gccttgagga gctccgagcc gcagaggccg acgaggatca
     1141 gcgccggatc gtaggcggcc accgcttcgg ctatggcggc ggcgaggtca gcgcgcttgg
     1201 ccgccatgtt gtacagcgcg ccgtgtggtt tgacatgcgt cagcgcgacg cctcgagcct
     1261 tggcgaaggc cccgagggcg cctatctggt agagcgtcat cgcgcgcgcc tcgtcggcgc
     1321 tcgccgatag ctcgcgccgt ccgaaaccca ggagatccgg gaagcccggg tgtgcgccga
     1381 tcgaaacgcc tgccgacgcg gccagcgcga ccgtacgctc catgaccgac gggtcgccgg
     1441 catggaagcc gcaggcgata ttggccgagg tcacgtaccg aagcacgccc tcgtcgtccc
     1501 cgatggagta ggcgccgaag ccttccccca ggtcgcagtt caggtctatt ctaggcatcg
     1561 atagtctcct cgccttcgat ggaagacgcg gcgcgaatgg cctcgagggc cgccaggttc
     1621 gactcggggc cgaggccgtc gacggtcggt tcgcgacgac ccgagcagcc gtcgacgccg
     1681 ccagccgccg ctacagcctc gagcatggcc gtttcgtcgc ccggtccgtg gaggagcgaa
     1741 atcgtccggc cgccctcgag cgccagcagc tcgagggccc tgacgagccc gtaagcgccc
     1801 cagttggaca cgccggctac gataggcgcg tcggcgggcg ttactgcccg aatcgtcgaa
     1861 ccgagcggga cgcgctcgtc tatgatggcc ccgatggagc ccagccccag ctcgttgcct
     1921 ccatcgccga tggcgacgga gacgccgcca tagtcggacg ccctggacac gagcgcatcg
     1981 gtgtcgggta cgagcgctcg catgtcggag ccgcgcatcg agtagaaacc gccgtctgcc
     2041 gccttgcctg gccgctcgat ggccacgacg acgtccgggc ggaagctcga caggatggcg
     2101 aggcaggcga gctcgccgtc gcccggagac agcgtcacga cgcgaggcct cgagccgagc
     2161 gcggcggccg atgcctcgac caaggcggcc gagtggaggt cggtgacgat acggacgtcc
     2221 ttgccgagga gggctaaggc cctcgccaaa acggcggagc caggcgggcc atcgctctcg
     2281 ccgactccgg cccgctcgat gaggaagccg gtgacgatca ggaccctgtc ggccccggcc
     2341 agcctggacg cggcccgccg gatagaaccc cgtagcgcga cgcgtaaccg cgggtcgccg
     2401 cataggcctc ggccgcccgg gtcgaaagcc atcgcggcct cgatcgacgc cgggccggcg
     2461 gcctcataag ttgaaacgct catgacactc ctcgtagccc gtttggggcg actaggtccg
     2521 gacgctgacg accttgccgc gcttgacgac ggtgccgacg acgttcatgc ccacatggta
     2581 cgccagatgg cggtacgacg gggcgtccag tacgagcagg tcggccagct tgccggcctc
     2641 tatcgagccc cggtcgcctg ccagggagag cgcggcggcg gcgttcaggg tcagcgcggt
     2701 caacgtctcc tcgatagtca tgcccatgta cagcaccgac aaggccgcgg cgaggggaat
     2761 ggcctggctg tagcacgagc cggg
//
LOCUS       DAOPHV010000359         2782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_50807_length_2782_cov_4.368904, whole genome shotgun sequence.
ACCESSION   DAOPHV010000359 DAOPHV010000000
VERSION     DAOPHV010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50807_length_2782_cov_4.368904"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="PLQ29_09380"
     CDS             <1..984
                     /locus_tag="PLQ29_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG86897.1"
                     /translation="LARSPRAFFERLATRTLYDDTQAQAVLGAAGVECPAFEAYAPAL
                     VAAAKQRLHARRVRPDAVAQEPEIDDPASPVRPVEAKGRVEFRNVDFGYDGASEPAVK
                     DVSFVAEPGTTTAIIGGTGSGKSTLINLLARFYDVKDGAVLVDGVDVRDMSQETLRSY
                     LGLAPQNATLFSGTIAENLRFGRDEATDDELAEAAAIAQASAFIAEKDGGMEATLSQG
                     GVNVSGGQKQRLSIARALARKPKIYLFDDSFSALDFKTDARLRSALRKKTGDATVIIV
                     AQRVGTIRDADQIVVLDEGRVVGKGRHAELMATCAVYKEIVASQLSAEEAV"
     gene            981..>2782
                     /locus_tag="PLQ29_09385"
     CDS             981..>2782
                     /locus_tag="PLQ29_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG86898.1"
                     /translation="MSAAETGKGGRAAPDIKPTQLLGRGPGGRGPGGHGPGGFGMPGE
                     KAKDFKGTTRRLVSYLRPHLPAILFALVMAVLSTVFSIAGPKILGQAITLLFEGVARS
                     AVDFRGIGRILALLLGLYLVSAGFNYLTQYSMAGVSQKTVYSFRKGIDEKLARLPLRY
                     FDGRTHGEILSRVTNDVDTIANTLQQSLVQIITSAVTLVGSVVMMLTISWKLTLVALA
                     TLPFYALATTAIAKRSQKYYKGQQKFLGELNGHVEEMYSGHQVVKAFGYEKRSVARFE
                     EVNDRLFDVAWKAQFVSGIIMPTMNFINNIGYVLIAAIGGVMAAARAISLGDVQAFIQ
                     YAKQFTQPIIQTANIANILQSTMAAGERVFELLDEAEESPDAPADSPAALAFAAASGQ
                     VSFEAVSFGYSDDTPLMDGLSLEAPRGEAVAIVGPTGAGKTTLVNLLMRFYELKGGRI
                     AVDGVDIRDVPRGRLRRAFGMVLQDAWLFGGTIRDNIAYGREGATEDEIVAAAVAAHA
                     DHFIRVLPDGYDTVLTEDATNLSQGQRQLLTIARAFLADPRILILDEATSSVDTRTEL
                     VVRKAMERLMRGRTSFVIAHRLSTIRDAHTILVM"
BASE COUNT          496 a          948 c          910 g          428 t
ORIGIN      
        1 ctcgcgcgca gtcccagggc cttcttcgaa cgcctcgcca cccgcaccct ctacgacgac
       61 acgcaggccc aggcggtgct cggcgcggcg ggcgtcgagt gccccgcctt cgaagcctac
      121 gcgccggccc tcgtcgcggc ggcgaagcag cgcctccacg cgcgccgcgt ccgccccgac
      181 gccgtcgcgc aggagcccga gatcgacgac ccggcctccc cagtcaggcc ggtcgaggcc
      241 aagggccgcg tcgagttcag gaacgtcgac ttcggctacg acggcgcgtc cgagcccgcc
      301 gtcaaggacg tgtccttcgt cgcggagccg ggcacgacca cggccatcat aggcggcacg
      361 ggctcgggca agtcgacgtt gatcaatctg ctcgcccgct tctacgacgt caaggacggg
      421 gcggtcctcg tcgacggcgt cgatgtgcga gacatgagcc aggaaacgct acgctcctac
      481 ctgggcctgg cgccccagaa tgccacccta ttctccggca ccatcgccga gaacctgcgc
      541 ttcggcaggg acgaggccac ggacgacgag ctggcagagg ccgcggcgat tgcccaggcc
      601 tccgcgttca tagcggagaa ggacggcggc atggaggcga ccctgtccca gggcggcgtg
      661 aacgtctcgg gcggccagaa acagcgcctc tcgatcgcga gggcgctggc gcggaagccc
      721 aagatatacc tgttcgacga cagcttctcc gcccttgact tcaagaccga cgcgcgcttg
      781 cgctcggcct tacggaagaa gaccggagac gcgacggtca tcatcgtcgc gcagcgcgtc
      841 ggcactatac gcgacgccga ccagatcgtg gtgctcgacg agggccgcgt cgtgggtaag
      901 ggcaggcacg ccgaactgat ggcgacctgc gccgtgtaca aggaaatcgt agcgtcgcag
      961 ttgtcagcgg aggaggcggt atgagcgccg cggagacggg gaagggcggg cgggccgcgc
     1021 ccgatatcaa gccgacccag ctgctcggca ggggcccggg cgggcgaggt cccggaggcc
     1081 acggcccggg aggtttcggc atgcccggag aaaaggccaa ggacttcaag ggcacgacga
     1141 ggcggctggt atcctacctt cggccacacc tcccggccat cctgttcgcg ctggtcatgg
     1201 ccgtcctgag taccgtgttc tcgatagccg gccccaagat tctcggtcag gccatcacgc
     1261 tgctgttcga gggcgtcgcc aggtcagccg tcgacttcag gggcataggc aggatactgg
     1321 cgctgctcct ggggctgtac ctggtctcgg ctggcttcaa ctacctgacc cagtactcga
     1381 tggccggcgt ctcccagaag acggtctact ccttccgcaa ggggatcgac gagaagctgg
     1441 cccgcctgcc tctcaggtac ttcgacggcc gtacccacgg cgagatcctg tcccgtgtga
     1501 ccaacgacgt cgacaccatc gccaacaccc tgcaacagag cctcgtgcag atcatcacct
     1561 cggccgtgac cctggtcggt agcgtcgtca tgatgctgac cataagctgg aagctgacgc
     1621 tcgtcgctct ggccaccctg cccttctacg cgctggccac gacggccatc gccaaacgct
     1681 cccagaagta ctacaagggc cagcagaaat tcctgggcga gctgaacggc cacgtcgagg
     1741 aaatgtactc gggccaccag gtcgtcaagg ccttcggcta cgagaagcgc tcggtagcgc
     1801 gcttcgagga agtgaacgac aggctgttcg acgtggcgtg gaaggcgcag ttcgtctcgg
     1861 gcatcatcat gccgacgatg aacttcatca ataacatcgg ctacgtgctg atcgccgcga
     1921 taggaggcgt gatggccgcc gccagggcga tcagcctggg cgacgtgcag gccttcatcc
     1981 agtacgccaa gcagttcacc cagcccatca tccagacggc gaacatagcc aacatactgc
     2041 agtcgacgat ggcggccggc gagcgcgtct tcgagctgct cgacgaggcg gaggagtctc
     2101 ccgacgcccc ggccgactcg ccggccgcgc tcgccttcgc ggccgcctcc ggccaggtct
     2161 cgttcgaggc cgtttcgttc ggctatagcg acgatacgcc gctcatggac ggcctgtcgc
     2221 tcgaggcgcc gcggggcgag gccgtagcga tcgtcgggcc gaccggcgcc ggcaagacga
     2281 ccctcgtcaa cctgctgatg cgcttctacg agctcaaggg agggcggatc gcggtcgacg
     2341 gcgtggacat cagggacgtg ccgcgaggcc ggctcaggcg cgccttcggc atggtgctgc
     2401 aggatgcctg gctgttcggc ggcaccatac gcgacaatat cgcctacggc cgcgagggcg
     2461 cgaccgagga cgagatcgtc gccgcggcgg tcgcggccca cgccgaccat ttcatccgcg
     2521 tactgcccga cggctacgac accgtgctga ccgaggacgc caccaacctg tcgcaaggcc
     2581 agcgacagct cctgaccata gcgcgcgcct ttctggccga tcccaggata ctcatcctgg
     2641 acgaggcgac cagttccgtg gatacccgta ccgaactggt cgtgcgcaag gccatggagc
     2701 gcttgatgag gggccgcacc agcttcgtga tagcgcaccg cctatcgacg atacgtgatg
     2761 cgcatactat cctcgtgatg aa
//
LOCUS       DAOPHV010000360         2774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_51024_length_2774_cov_2.632218, whole genome shotgun sequence.
ACCESSION   DAOPHV010000360 DAOPHV010000000
VERSION     DAOPHV010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51024_length_2774_cov_2.632218"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PLQ29_09390"
     CDS             complement(<1..593)
                     /locus_tag="PLQ29_09390"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPG86899.1"
                     /translation="MMVFGFIPGELIMIVISCVLLYMGIARKFEPLLLVPIAFGMLLS
                     NLPFGGVFTPADGAQPGGLIYYLYYGVKFGIYPPLIFLGVGAMTDFGPLIANPKSLLL
                     GAAAQIGIFIPFVLAFYLGKAFPSLGLDLKAAASIGIIGGADGPTAIYLTSKLKPELL
                     GPIAIAAYSYMALVPLIQPPIMKALTTKRERQIKMEQL"
     gene            complement(679..1059)
                     /locus_tag="PLQ29_09395"
     CDS             complement(679..1059)
                     /locus_tag="PLQ29_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPG86900.1"
                     /translation="MVKKYKVTVNGQSYDVGVEELGASASYAAPVAQAPAAAAPVAPV
                     PAAAPAAPAPAGATTVKAPMPGTVLSIKVAAGQAVKRGDVLLILEAMKMENEISAPAD
                     GVVAGIRVQAGSTVNTGDPMVDLA"
     gene            complement(1091..1438)
                     /locus_tag="PLQ29_09400"
     CDS             complement(1091..1438)
                     /locus_tag="PLQ29_09400"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HPG86901.1"
                     /translation="MDTVAVTETAGQMWTIVVVGIGTVFLCLFALVVMVSVFKAIFVR
                     EPKAAPKPAAQAAPVQVASAQAAARGLDGAVVAAIVAAVSAASGVPVSALRIASIERS
                     GFNTPAWGHIDRA"
     gene            complement(1449..>2774)
                     /locus_tag="PLQ29_09405"
     CDS             complement(1449..>2774)
                     /locus_tag="PLQ29_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPG86902.1"
                     /translation="VVIGYGTVNGRLVYLFAQDFTVIGGSLGEMHAAKICKVMDMAMK
                     VGCPCIGINDSGGARIQEGVDALYGYGNIFYRNTLASGVIPQISVIMGPCAGGAVYSP
                     ALTDFTVMVDKTSNMFITGPQVIKAVTGEDVSAEALGGALVHSETSGCASLMFADEKA
                     TIEGVRKLLSYLPQNNVEDAPIVSGGDDVARACPGLVGIIPDSPNKPYDIRDVIAQVF
                     DDGDYFEIQPLYAKNIVTCFARLDGRSVGVIANQPKVMAGVLDINASDKASRFIRFCD
                     SFNIPLVTLTDTAGYLPGVGQEHGGVIRHGAKLLYAYSEATVPKITVIVRKAYGGAYI
                     AMCSRHLGADQVFAWPSAEIAVMGPEGAANIIFKKEIDEAADPAAARKDKIQEYKDNF
                     ANPYKAAARGYVDDVIDPAVTRARLASALTMLLGKREKRPAKKHANMPL"
BASE COUNT          477 a          858 c          948 g          491 t
ORIGIN      
        1 agctgctcca tcttgatctg gcgctcgcgc ttggtggtca gcgccttcat gatcggcggc
       61 tggatgagcg gcacgagcgc catatacgag taggcggcta tcgctatagg cccgagcagc
      121 tctggcttga gcttactggt cagatagatc gccgtcgggc cgtccgcgcc gccgatgatg
      181 ccgatgctcg ccgcggcctt gaggtctaag ccaagcgacg ggaaggcctt gcccaggtag
      241 aacgccagca cgaacggtat gaagatgccg atctgcgcgg cggctccgag gagcaggctc
      301 ttggggttcg cgatgagcgg gccgaagtcc gtcatcgctc cgactcccag gaagatgagc
      361 ggcgggtata tgccgaactt gacgccgtag tacaggtagt agatgaggcc gcccggctgc
      421 gcgccatcgg ccggcgtgaa gacgccgccg aaaggcaggt tcgacagtag catgccgaag
      481 gctatcggta cgagtagaag cggctcgaac ttcctagcaa tacccatgta caggagcacg
      541 cacgagatca cgatcatgat gagctccccg ggtatgaacc cgaagaccat catctggctg
      601 ttcgcgaagc cggcgtaccc ggatgtctgg aagaactcca gtatattctg ccacatacgc
      661 tattcccttc gccccgggtc aggcgaggtc gaccatcggg tctcctgtgt tgacggtcga
      721 gccggcctgg acgcggatgc cggcgacgac gccgtcggcg ggggcgctga tctcgttctc
      781 catcttcatg gcctcgagga tcaggagcac gtcgccgcgc tttacggcct ggcccgcggc
      841 caccttgatg gacagcacgg taccgggcat cggagcctta acggtagtcg cgcctgcagg
      901 cgcgggagcg gcgggggccg cggcgggaac cggggctacg ggagcggccg ccgcgggcgc
      961 ctgggcgacc ggggccgcgt acgaagcgga agcgccgagc tcctcgacgc cgacgtcgta
     1021 ggactgaccg ttgaccgtaa ccttatattt cttgaccatg actgactcct ttgtttcgta
     1081 gtgccgcgcc tcaagcgcga tcgatatggc cccacgcggg ggtattgaag cccgagcgct
     1141 caatggacgc gatcctgagc gcggagaccg gcacgccgct cgcggccgag accgcggcga
     1201 cgatggccgc cacaacggct ccgtccaggc ctcgagcggc ggcctgagcc gacgctacct
     1261 ggaccggagc ggcttgagcc gctggcttcg gcgcggcctt cggctcgcgc acgaagatcg
     1321 ccttgaacac gctgaccata acgacgaggg cgaagaggca caggaacacc gtgcctatgc
     1381 ccacgacgac tatggtccac atctgccccg ctgtctcggt gactgctacg gtatccatag
     1441 cgtccccttt acagcggcat gttggcgtgc ttcttggcgg ggcgcttctc gcgcttgcca
     1501 aggagcatcg tcagggccga ggcgagcctg gccctcgtga cggccgggtc gatcacgtcg
     1561 tcgacgtagc cgcgagcggc cgccttgtac ggattggcga aattatcctt gtactcctgg
     1621 atcttgtcct tgcgagccgc cgcaggatcc gccgcctcgt cgatctcctt cttgaagatg
     1681 atattggcgg cgccctcagg gcccatcacg gcgatctcgg cgctcggcca ggcgaagacc
     1741 tggtcggccc cgaggtggcg cgagcacatg gcgatgtacg cgccgccgta ggccttgcgc
     1801 acgatcacgg tgatcttcgg cacggtcgcc tcggagtagg cgtacaggag cttggcgccg
     1861 tggcgtatca cgccgccgtg ctcctggcct acgcccggca ggtagccggc ggtgtcggtc
     1921 agcgtgacga gggggatatt gaacgagtcg cagaagcgga tgaagcggct ggccttgtcc
     1981 gaagcgttga tgtcgagcac gccggccatc accttgggct ggttggctat gacgccgacc
     2041 gaacgaccgt cgagtctggc gaagcaggtg acgatgttct tggcgtagag gggctggatc
     2101 tcgaaatagt cgccgtcgtc gaagacctgg gctatcacgt cgcggatgtc gtagggctta
     2161 ttgggcgagt cggggatgat gccgacgagg cccgggcagg cgcgagcgac gtcgtcgccg
     2221 ccggagacga tcggcgcgtc ttccacgttg ttctgcggta ggtacgacag cagcttgcgc
     2281 acgccctcga tggtcgcctt ctcgtcggcg aacataaggc tggcgcagcc ggaggtctcg
     2341 ctgtgcacca gggcgccgcc gagggcctcg gccgaaacgt cctctccggt cacggccttg
     2401 ataacctgcg gaccggtaat gaacatgttg gaggtcttgt cgaccatcac ggtgaagtcg
     2461 gtcagggccg gggagtagac ggcgccgccg gcgcaggggc ccatgatcac cgaaatctgc
     2521 gggatcacgc cggaggccag cgtgttgcgg tagaagatgt tgccgtagcc gtacagcgcg
     2581 tcgacgcctt cctggatgcg cgcgccgccc gagtcgttga tgccgatgca ggggcagccg
     2641 accttcatgg ccatgtccat caccttgcag atcttggcgg cgtgcatctc gcctaaacta
     2701 ccgccgatga cggtgaagtc ctgcgcgaac aggtagacga ggcggccgtt gaccgtgccg
     2761 tagccgatga cgac
//
LOCUS       DAOPHV010000361         2770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_51118_length_2770_cov_3.225046, whole genome shotgun sequence.
ACCESSION   DAOPHV010000361 DAOPHV010000000
VERSION     DAOPHV010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51118_length_2770_cov_3.225046"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLQ29_09410"
     CDS             <1..347
                     /locus_tag="PLQ29_09410"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HPG86903.1"
                     /translation="GQARKVMNFMWGMGQMMYTKLIVVVDDDVKPSDVSTVAWRAFNN
                     IDARRDLVFSDGPLDALDHSSPLPRYGTRLGIDATRKGPEEGHTRPWPSPLSMDEHIK
                     SLVDGRWEQYGL"
     gene            337..1227
                     /locus_tag="PLQ29_09415"
     CDS             337..1227
                     /locus_tag="PLQ29_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HPG86904.1"
                     /translation="MASEPGSAASRGAWRRLGDIGDAVIVRHTLFSLPFAVVAILLET
                     DGRPPIGKLILILVAAMAARNAANALNRVVDAEIDARNPRTAGRHLPTGRLSKKALVA
                     FTVAMGAILVAATAMLNWLCVALLPVAAVLIGGYSYTKRFTWLCHYWLGVACAAAPMG
                     AFLALSGRFELGYFALAGAVALWVAGFDILYALQDIDFDRSAGLHSVPARFGPGGARL
                     IAALSHLGTVGALAALPLFWKLSWPYLAGVAVAAAMLAAEHLIARGGTERHIRVASYS
                     INEILPLVVLAATAVDVYLA"
     gene            1239..1871
                     /locus_tag="PLQ29_09420"
     CDS             1239..1871
                     /locus_tag="PLQ29_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HPG86905.1"
                     /translation="MGRYLVCVTGASGAVYGKRTIEALWSSGHEVHAVFSAWGRRVFE
                     TETGLSLDAWLDGIAFPRDRLYAPDDLAAPPSSGSWRLDGAAVVPCSMSSLGAIASGA
                     TTNLVHRAAAVALKEGRRLVLVPRETPLSLVDLRNLTALAEAGAAILPACPGFYHGPE
                     TIADLVDHVVGKILDRLIVPFNGVGSAYSGDAARGPDELFRRWGGDTRES"
     gene            1994..>2770
                     /locus_tag="PLQ29_09425"
     CDS             1994..>2770
                     /locus_tag="PLQ29_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86906.1"
                     /translation="MKHQRLLAWLLISAAVSCYAQVAENFSFHVRPELDLPIGEKSAL
                     FFDNAAYRIGGNAALYGQYLFPKAPFLYAEGGATFGLHPTQAADALTLASAGVGLGCD
                     LRILDRVSLQLGAQGGYSFGFFGAYDPAGNPYLGASLAANLDLSPGLALSAGGAYRYH
                     VGWDPAAEAFTDLYQGVGVWAGAILRLQPDSGRRKLRIADLRTEPLFPVFFGYYEHNP
                     FGTMTVSNLENSAITNVDVYFYVGEYMEQPMLTVIVPKGLC"
BASE COUNT          415 a         1011 c          881 g          463 t
ORIGIN      
        1 ccggccaggc ccgcaaggtg atgaacttca tgtggggcat gggccagatg atgtacacga
       61 agctcatcgt cgtggtcgac gacgacgtga agccgtccga cgtgtcgacg gtcgcctggc
      121 gcgccttcaa taatatcgac gcgaggcgcg acctcgtgtt ctccgacggc cccctcgacg
      181 cgctcgacca ctcgtcgccg ctaccgcgct acggcacacg gctcggcatc gacgcgacgc
      241 gcaagggccc cgaggaaggg catacgcggc cgtggccatc gcccttgtcc atggacgagc
      301 atataaagag cctagtagat ggccgctggg agcagtatgg cctctgagcc cggctccgcc
      361 gcgagccggg gcgcatggcg ccgcctgggc gacataggcg acgcggtcat cgtcaggcat
      421 accctgttct cgctgccctt cgccgtcgtc gccatcctct tggagacgga cgggcggccc
      481 ccgatcggca agctgatcct gatactcgtc gcggcgatgg ccgcgaggaa cgcggccaac
      541 gcgctcaacc gcgtggtcga cgccgagatc gacgccagga acccgcgcac cgccggccgc
      601 cacctgccga cgggccggct gtccaagaag gcgctggtcg cgttcacggt cgccatgggc
      661 gccatcctcg tcgcggcgac cgcgatgctc aactggctgt gcgtggccct gctgccggtc
      721 gccgccgtcc tgataggcgg ctactcgtac acgaagcgct tcacctggct ctgccactac
      781 tggctcggcg tcgcctgcgc cgcggctccg atgggcgcct tcctggccct gtcgggccgc
      841 ttcgaactcg gttacttcgc tctggccggc gcagtggcgc tctgggtggc cggcttcgac
      901 atcctgtacg cgctccagga catcgacttc gaccgctcgg ccgggctcca ttcggtgccg
      961 gccaggttcg gccccggcgg cgccaggctg atcgccgccc tgtcccatct cggcaccgtc
     1021 ggcgccctgg ccgccttgcc cctgttctgg aagctgtcct ggccgtacct ggccggcgtc
     1081 gcggtggcgg cggccatgct ggcggccgag cacctgatcg cgcggggcgg caccgaacgc
     1141 catatccgcg tcgcgagcta ctcgatcaac gagatcctgc ccctcgtcgt gctggccgcc
     1201 acggccgtcg acgtgtacct ggcctaaggg aggcgagcat gggacgatac ctggtctgcg
     1261 tgaccggcgc gagcggcgcc gtctacggca agcgcacgat agaggcgcta tggtcgtccg
     1321 gccacgaggt ccacgcggtg ttctccgcct gggggaggcg cgtcttcgag acggagaccg
     1381 ggctatcgct cgacgcgtgg ctcgacggaa tcgcgttccc gcgcgatcgg ctctacgccc
     1441 cggacgacct ggccgcgcca ccgtcgtccg gctcctggcg gctcgacggc gccgcggtcg
     1501 tcccctgctc gatgagcagc ctcggcgcga tcgcctccgg cgcgacgacg aacctggttc
     1561 accgcgcggc cgcggtcgcc ctgaaggagg gccgccgcct cgtgctcgtg cctcgcgaga
     1621 cgccgctgtc cctcgtcgac ctgcgcaacc tgacggcgct ggccgaggcc ggcgccgcca
     1681 tcctgcccgc ctgtcccggc ttctaccatg gccccgagac gatagccgac ctcgtcgacc
     1741 acgtcgtcgg caagatcctg gataggctca tcgtcccgtt caacggcgtc gggagcgcct
     1801 actccggcga cgcggcgagg ggcccggacg agctgtttag gcgctggggc ggcgatacgc
     1861 gcgaatcatg agcgacgcgg gggaaaaccc tggaaaacgc ccatgcgccg cactacactc
     1921 atagcagtac cattctcatg cgttaaataa tctaaacata gcttcccatc cccatccacc
     1981 gggatggctt gtcatgaagc accagcgcct gctcgcctgg ctactgatct cggccgcggt
     2041 ttcctgctac gcccaggtag ccgagaattt ctcgttccat gtcaggccgg agctagacct
     2101 gcctataggc gaaaagagcg cgctgttctt cgacaacgcg gcctatcgaa taggcggcaa
     2161 cgccgcgctc tatggccagt acctgttccc caaggcgccc ttcctgtacg ccgagggagg
     2221 cgcgaccttc ggcctccatc cgacccaggc cgccgacgcc ctgaccctgg cctcggccgg
     2281 cgtcggcctc ggctgcgacc tgaggatact cgaccgcgtc agcttgcagc tcggagccca
     2341 gggcggctac tcgttcggct tcttcggcgc ctacgacccc gcgggcaacc cctacctcgg
     2401 cgctagcctc gccgccaact tggacctgag ccccggcctg gccttatcgg caggcggcgc
     2461 ttaccgctac cacgtcggct gggatcccgc cgccgaagcc tttaccgacc tgtaccaagg
     2521 cgtcggcgtc tgggccggcg cgatactcag gctccagccc gactccggcc gtcgcaagct
     2581 caggatagcc gacctgcgga ccgagccgct cttccccgta ttcttcggct actacgagca
     2641 taaccccttc ggcacgatga ccgtctccaa cctcgagaac agcgcgatca ccaacgtcga
     2701 cgtgtacttc tacgtcggcg agtacatgga acagccgatg ctgacggtca tcgtgccgaa
     2761 ggggttatgc
//
LOCUS       DAOPHV010000362         2769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_51147_length_2769_cov_3.392778, whole genome shotgun sequence.
ACCESSION   DAOPHV010000362 DAOPHV010000000
VERSION     DAOPHV010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51147_length_2769_cov_3.392778"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..712
                     /locus_tag="PLQ29_09430"
     CDS             227..712
                     /locus_tag="PLQ29_09430"
                     /inference="COORDINATES: protein motif:HMM:NF018958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HPG86907.1"
                     /translation="MMTNDILRRLRYSLRLSDDAMLEMLSLAGSPIGKDRLLSYFKRD
                     DEPGYAECPKAALSSLLDGMILKFRGPGPKGAAPATVEPLDNNMVLKKLRIALALKED
                     DMLSVMRLGEAELSAGELRALFRGRGQRNYVECLDQYLRGFLTGLARRSDGDDATRKS
                     V"
     gene            729..1811
                     /locus_tag="PLQ29_09435"
     CDS             729..1811
                     /locus_tag="PLQ29_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG86908.1"
                     /translation="MVGRPSRRSALPGRLRFIVKRAIRTLGAFAASMLCLSAVVNASF
                     EATVRSQIDEAAMGAVMASGSSSPEAILALRAEIRAGLERSYGLDAPSWLRVVRQTGR
                     ILRLDLGEARFDRTAYPEHSREVRRIVMERLPHTLALFTLSTTISIVVGIALGTAMAR
                     KPGGRLDRASSLTAMILYGAPSWWIGLFMILLLGYTFPVFKVGALHSPEVAPGRLAWA
                     LDYLAYLALPLITLVLIKLFSFAYLTRAMVILPLHEDYVFAARGRGLPEGRVVSRHGL
                     RVAAPGLTTMAAQTFAQSIVGDILVEKIMMRPGLGLTLFSAISSNDTALFTGVLAMVT
                     AVYCLSFLFLDIAYAWLDPRIAYGRE"
     gene            1972..2526
                     /locus_tag="PLQ29_09440"
     CDS             1972..2526
                     /locus_tag="PLQ29_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HPG86909.1"
                     /translation="MASFERTNPRFALCGLNCCLCPRYTTEGASRCPGCGGPGFSDIH
                     PTCAVLTCNKKRDDVEFCFRCGSYPCRKYLEQGAYDSFISYRRVQHNLDEAAADLAAY
                     EAELARREEALGELLRDYNDGRSKNLYCIVANDAPLDELETLIDSARSLSGDLDLKEK
                     ARRVRSEIQALERRLGFEFKLRRP"
     gene            2523..>2769
                     /locus_tag="PLQ29_09445"
     CDS             2523..>2769
                     /locus_tag="PLQ29_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86910.1"
                     /translation="MNRRTVIALGYLGLLLSATAVSAAQGPSPESDAVTASALSPPAR
                     LEYYGELGCSHCDTFMDRDLPAAEAASGVRVELEAFDI"
BASE COUNT          465 a          893 c          897 g          514 t
ORIGIN      
        1 gtcgaccgcc ggaaggcctt ctagtgtcct gagcacggcg aacaggttca cccgcgccga
       61 tgtcgttgga tcgaccatac gttttgcccc cctcgagttc gatgaaacgt atcgagcgaa
      121 tgctcgctcg attagttaga ccacgatttt tcgatttgcg caacaagaat cttcgcgttg
      181 agcgcggttg ctcgaggagc ctgaaccggt ataccatcgc cttaccatga tgaccaacga
      241 catactcagg cgcctacgat attccctccg gttgagcgac gacgcgatgc tcgagatgct
      301 ctccctggcc ggctccccga taggcaagga ccggctacta tcgtatttca agcgcgacga
      361 cgagccgggc tacgccgagt gcccgaaagc cgcgttgtcg tcgctactcg acgggatgat
      421 cctcaaattc agggggccgg ggccgaaggg cgccgcgccg gctaccgtcg agccgctcga
      481 caataacatg gtactcaaga agctcaggat agccctggcg ctcaaggaag acgatatgct
      541 gtcggtcatg aggctcggcg aggcggagct gagcgccggc gagctgcgcg cattgttccg
      601 gggcaggggg cagcggaact acgtcgagtg cctcgaccag tacctgcgcg gctttctgac
      661 cggcctcgcg aggcgctcgg acggggacga tgcgacgcgg aagtccgtct agcccatgcg
      721 gaagctggat ggtaggaaga ccgtctcgcc gctcggccct gccgggccgg ctcaggttca
      781 tagtaaagag ggccatacgg acgctcgggg ccttcgcggc ctcgatgctc tgcctctcgg
      841 ccgtggtcaa cgcctcgttc gaagccacgg tgcgctctca gattgacgaa gccgccatgg
      901 gagcggtgat ggccagcggt tcgtcgtcgc ccgaggcgat actggcgctc agggccgaga
      961 tccgcgccgg cctcgaacgt tcgtacggcc tcgatgcccc ctcgtggcta cgggtggtca
     1021 ggcagacggg ccgtattctg agactggatc tcggcgaggc ccgattcgac cggacggcgt
     1081 atccggagca ctcccgggag gtgcggcgca tcgtcatgga gcgcctaccg catacgctgg
     1141 ccctgttcac gttgagtacg acgatcagca tcgtcgtcgg catcgccctg ggcacggcga
     1201 tggccaggaa gccgggcggg cggctcgacc gggcctcgag cctgaccgcg atgatactct
     1261 acggggcgcc gtcctggtgg ataggcttgt tcatgatcct gctcctcggc tatacgttcc
     1321 cggtcttcaa ggtcggtgcg ctccattctc cggaggtcgc gcccggccgg ctggcttggg
     1381 cgctcgacta tctcgcatac ctggccttgc cgcttatcac gctcgtgctg atcaagctgt
     1441 tcagcttcgc ctacctgacg cgggccatgg tcatcctgcc gttgcacgag gattacgtct
     1501 tcgccgctcg cggtaggggc ctgcccgagg gccgggtcgt gagccggcac gggctacgcg
     1561 tggccgctcc gggcttgacg acgatggccg cgcagacctt cgcgcagtcc atcgtcggcg
     1621 acatcctcgt cgaaaagatc atgatgcgcc cggggctcgg cctgacccta ttctcggcca
     1681 tatcgtcgaa cgatacggcc ttgttcaccg gcgtcctggc gatggtcacc gccgtctact
     1741 gcctcagttt cctgtttctc gatatagcgt acgcctggct ggacccgcgc atcgcgtacg
     1801 gcagggaata gctcaggatc ccgcgccgcc tattgacagc ccggcctcct tgcagcaagg
     1861 tcgacgaggg actggggcga cgggccgggg cgacacatcg aggcgacaag tcggggcgac
     1921 aggtcgaggc gacgggccga gtacaccgtc tgcggtttcc gaggagggag catggcgagc
     1981 ttcgaacgaa cgaatccgcg cttcgcgctc tgcggcctca actgctgtct ctgcccgcgc
     2041 tacactaccg aaggcgcgtc gcgctgcccg ggttgcggtg gtccaggctt ttccgatatc
     2101 cacccgacct gcgccgtcct cacctgcaac aagaagcgcg acgacgtgga attctgcttc
     2161 cgatgcggct cgtacccctg ccgtaagtac ctggaacagg gggcgtacga ctcgttcata
     2221 tcgtataggc gggtacagca taacctcgac gaagccgcgg ccgacctggc cgcgtacgag
     2281 gccgagctcg cccgtcgcga agaggccctc ggggagctcc tccgcgatta caatgacggg
     2341 cgctccaaga acctgtactg catcgtcgcg aacgacgccc cgctcgacga gctcgagact
     2401 ctgatcgata gcgccagaag cctatccggc gacctggacc tcaaggagaa ggcgaggcgg
     2461 gtacgttccg agatacaggc tcttgaacgt cgcctcggtt tcgagttcaa gctgaggcgg
     2521 ccgtgaatcg acgtacggtc atcgctctcg gctatctggg gcttcttctg tcggccacgg
     2581 ccgtctcggc tgcccaggga ccgtcgcccg agtccgatgc cgtcaccgcg tcggccttaa
     2641 gcccgcccgc tcgcctcgag tactacggcg agctcggttg ctcgcactgc gataccttca
     2701 tggataggga cctgccggcg gccgaggctg cgagcggggt gcgggtagag ctggaagcgt
     2761 tcgacatcc
//
LOCUS       DAOPHV010000363         2767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_51199_length_2767_cov_3.428097, whole genome shotgun sequence.
ACCESSION   DAOPHV010000363 DAOPHV010000000
VERSION     DAOPHV010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51199_length_2767_cov_3.428097"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLQ29_09450"
     CDS             complement(<1..377)
                     /locus_tag="PLQ29_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPG86911.1"
                     /translation="MPDRRRLVVIVTGASSGLGNACATQLAKRGYAVYGTSRDPASRE
                     RKADEFFELIRLESSDDDSVSTAVGYVLAKEGRLDALVCCAGMGIAGPIEETPVVEAA
                     TQMDVNFMGVARVVRAALPSLRRA"
     gene            complement(370..927)
                     /locus_tag="PLQ29_09455"
     CDS             complement(370..927)
                     /locus_tag="PLQ29_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG86912.1"
                     /translation="MSDFTESARKPSIIAISGKSGCGNSTVSRLVTERLGRQLVNYTF
                     HTMADEMGVPFAELLEMAKADPSYDRRLDERQVEMAHRGDCVIGSRLAIWLVRDAELK
                     VYLLASSDVRARRIQQREGGEYADVLAFTKRRDESDRERYLSIYGIDNDRYDFADLVI
                     NTERLSAERIADIVIAAIGRGPDCA"
     gene            complement(924..1787)
                     /locus_tag="PLQ29_09460"
     CDS             complement(924..1787)
                     /locus_tag="PLQ29_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPG86913.1"
                     /translation="MKSMTGYGSARLDSPAIKASIVAKSWNNRFLELAIQMPGYLGPL
                     ERRVRELVERRVARGKLELSVRVLGGEMPTDVVIDASSAKAVADAIRRLGQAAGIDEP
                     VRLSHLLGVDGMLSYERNVDADLLWTALEPALNACLDEFDAEREREGAATRVDLEEKL
                     ATLGWALDVIEAAGPGIEAGLKAALRAKFEEVMGDLVDESRVLGELASYLAKHTINEE
                     IVRLRSHMAAFRQAMGEPVCGKKLDFICQEMNREANTIGSKSADARVSDAVIRMKDAV
                     ENIREQARNVE"
     gene            complement(1784..>2767)
                     /locus_tag="PLQ29_09465"
     CDS             complement(1784..>2767)
                     /locus_tag="PLQ29_09465"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPG86914.1"
                     /translation="AETCEYRRSFMAFKPRRVVLTSVEPDHQDYYPDYEAIRAAFVEY
                     LVALPPAGTVIYCADDPGAVDAWTRAEAARPDLRGIPYGFSAPGDFGMEGYRVERERG
                     IFKLRGFGLEWRLRVPGRHVALDAAGAVALIAAIVADRDGARRLSEDELSAMAEALES
                     FSGSRRRSEVLGEVGGILFMDDYGHHPTAIRSTLAGLREFYPERRLVVDFMSHTCSRT
                     RALLDEFASSFADADLLVLHRIYPSAREAPDPETTGRLLYERTVSATALAGRPSRQYY
                     EDPLDALSFLSGELRPGDLFVTMGAGDNWKLGRALYEAGKAAAEARVKGRP"
BASE COUNT          420 a          953 c          931 g          463 t
ORIGIN      
        1 gctctccgta ggctgggcag ggcggccctg accacgcgag cgacgcccat gaagttcacg
       61 tccatctggg tagccgcctc gacgacgggc gtctcctcga tcgggcctgc gatgcccatg
      121 ccggcgcagc atacgagggc gtccagacgt ccctccttgg ccagtacgta gcccacggcg
      181 gtcgatacgg agtcgtcgtc cgacgactcg agccgtatca gctcaaagaa ctcgtccgcc
      241 ttgcgctcgc gcgaggcggg atcccggctc gtaccgtaga cggcgtagcc gcgcttggcc
      301 agttgggtgg cgcaggcgtt gccgaggccg ctcgacgcgc cggtgacgat gacgaccagc
      361 cggcgacgat caggcacagt ccggccctcg gcctatggcc gcgatgacga tgtccgctat
      421 ccgctcggcg ctcaggcgct cggtgttgat gaccaggtcg gcgaagtcgt agcggtcgtt
      481 gtcgatgccg tagatcgaca ggtagcgctc gcggtccgac tcgtcgcgac gcttcgtgaa
      541 cgcgagtacg tcggcgtact cgcctccctc gcgctgctgg atgcgcctgg cgcgcacgtc
      601 gctcgaggcg aggaggtaga ccttgagttc ggcgtcacgg accagccaga tggcgagcct
      661 cgagccgatc acgcagtcgc cgcggtgggc catctcgacc tgccgctcgt cgagcctgcg
      721 atcgtacgac ggatcggcct tggccatctc caggagctcg gcgaacggca cgcccatctc
      781 gtccgccatc gtatggaagg tatagttgac gagctggcgt ccgagccgct cggtgaccag
      841 cctcgagacg gtcgagttgc cgcagccgct cttacccgat atggcaatga tcgacggctt
      901 cctggcgctt tcggtgaaat cgctcattcg acgttcctcg cctgctcgcg gatgttctcg
      961 accgcgtcct tcatgcggat gaccgcgtcc gaaaccctcg cgtcggcgct cttggagccg
     1021 atggtgttgg cctcgcgatt catctcctgg cagatgaagt cgagcttctt gccgcagacc
     1081 ggctcgccca tcgcctggcg gaaggcggcc atatgcgagc gcagcctgac tatctcctcg
     1141 ttgatcgtat gcttggccag gtaggaggcc agctcgccga gcacgcgcga ctcgtcgacc
     1201 aagtcgccca tgacctcctc gaacttggcc cgcaaggccg ccttcaggcc ggcctcgatg
     1261 ccggggcccg ccgcctcgat cacgtcgagg gcccagccga gggtcgccag cttctcctcg
     1321 aggtcgaccc gggtggctgc tccctcgcgc tcgcgctcgg cgtcgaactc gtcgaggcag
     1381 gcgttcaggg ccggttccag ggcggtccag agcaggtccg cgtcgacatt gcgctcgtag
     1441 ctcagcatgc catcgacgcc gagcaggtgc gaaagcctga ccggctcgtc gatcccggcc
     1501 gcctggccga gcctgcgtat ggcgtccgct accgccttgg cgctcgaggc gtcgatgacc
     1561 acgtcggtgg gcatctcgcc accgagcacc ctgaccgaca gctccagctt gcccctggcc
     1621 acgcggcgct ccacgagttc gcgcacccgt cgctcgagcg gcccgaggta gccgggcatc
     1681 tggatcgcca gctcgaggaa ccggttattc caggacttgg cgactatcga cgccttgatg
     1741 gccggcgaat cgagccgcgc cgaaccgtat ccggtcatgc tcttcatggt cgtcctttca
     1801 cgcgcgcttc ggccgcggcc ttcccggcct cgtacagggc gcggcccagc ttccagttgt
     1861 cgccggcgcc catcgtgacg aagaggtcgc cgggtcgcag ctctccggac aggaacgaca
     1921 gcgcgtcgag cgggtcttcg tagtactgac ggctcggcct gccagccaag gcggtggccg
     1981 agacggtgcg ctcgtagagc aaccggcctg tcgtctccgg gtcgggagcc tcgcgggccg
     2041 atggatagat gcgatgcagc acgaggaggt cggcgtcggc gaacgacgag gcgaactcgt
     2101 cgagcagggc cctcgtcctc gagcaggtat gcgacatgaa gtcgacgacg aggcgtcgct
     2161 ccgggtagaa ttcgcggagg cctgccaggg tggatcgtat ggcggtcgga tggtggccgt
     2221 agtcgtccat gaacaggatc ccgccgacct ccccgagcac ctccgacctg cggcgcgagc
     2281 cggagaacga ctccagagcc tctgccatgg cggacagctc gtcctcggaa agccgccgag
     2341 caccatccct atcggcgacg atggcggcta tcagcgctac ggcgcccgcg gcgtcgagcg
     2401 cgacgtgccg gccgggcacc cggagccgcc actcgaggcc gaagccgcgc aacttgaaga
     2461 tcccgcgctc acgctccacg cggtagcctt ccatgccgaa gtcgccgggc gctgagaacc
     2521 cgtagggtat gcctcgcagg tcgggcctgg cggcctcggc ccgcgtccag gcatcgacgg
     2581 ctccggggtc gtcggcgcaa tagatcaccg tcccggcggg cggcagggcg accaggtact
     2641 cgacgaaggc cgcgcggatg gcctcgtagt ccggatagta gtcctggtgg tccggctcga
     2701 cgctcgtcag cacgacgcgg cgcggcttga aggccatgaa gctcctgcgg tactcgcagg
     2761 tctcggc
//
LOCUS       DAOPHV010000364         2765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_51241_length_2765_cov_4.597417, whole genome shotgun sequence.
ACCESSION   DAOPHV010000364 DAOPHV010000000
VERSION     DAOPHV010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51241_length_2765_cov_4.597417"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1966
                     /locus_tag="PLQ29_09470"
     CDS             <1..1966
                     /locus_tag="PLQ29_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPG86915.1"
                     /translation="TMQIILGAFRTPEGSFTLARTAGGAIDGTVNLTASGATPNATAE
                     LWGLELYPNTSYLVTLSGAVQDIAGNPLGADKTWYFSTGDAEDTSPPTGAVTLTDQGG
                     AVIVVTLPSGSIATDDATLELDFSAITDDYNIPYGIKIWGDNGNAAAFFEEDASWRTW
                     SDTVSWTLSAGSGTKYVLYKLMDSANNQSVTPRQLRIILDSGELPTIATVSVNGGQSH
                     TNASDRAVDLTIEASDAYSGVKQMIISHDPSFAGASWMNYTPILEGWVLPDGDGTRIF
                     YAKVRDYLDQESATNQACSITLDRAAPSVSFNVESILASEPTLLAEGSEGSDLYRIIE
                     EFGVASCAWEQLSGQGTLYFNADDGGSTDNDGIGLREPYIHATVEGSYFARVTVTDNA
                     GNSSQATIPFSWDVTPPADLAAVNVSAYDTSGQPTWSWATVADADYYRASYSSDFSSY
                     VDVHSTSFTPNAPLAPDGVKTLYVRAQDEAGNYSTTRSASTHVDTTAPTISVTTNSFV
                     ANVAAPTATLAFNGTHGSVADSGSGVQSGLDTSSYLWEELSTPKYLSFGSASALSTTV
                     SASIDKTYQIRFRARDLAGNVSYATVSLLRDTNPPAATAVTGPAITPSLRPTWSWSSS
                     SGGAGLFQFYLTNDSDSTTVYTSGETTVKN"
     gene            2006..>2765
                     /locus_tag="PLQ29_09475"
     CDS             2006..>2765
                     /locus_tag="PLQ29_09475"
                     /inference="COORDINATES: protein motif:HMM:NF039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPG86916.1"
                     /translation="MRVRERDGAGNWSAYGTRTTVVDSSATTPAQIALGDGYPALRTV
                     ASVEWDLLTGCGGAANLYRYDVDSTGTWTYGGAALSENVPVTISRSGLTEGAHTIVVQ
                     ERIDTAWRTDIQASHTITVDTVKPTEPTLTGTGLATGQSYRTATPDTTPTWIWSPGGG
                     GGNGVYSYRVTRVYTAAGIADGAVIVAQTAETAAMSYTPSSALAEGTYALSVRERDEA
                     GNWSLDATQYTTVDRTKPTMVGVLVRGPTHPDDTD"
BASE COUNT          531 a          974 c          852 g          408 t
ORIGIN      
        1 gacgatgcag atcatcctcg gcgcctttcg cacccccgag ggcagcttta cgctcgcgag
       61 gacggccggc ggcgccatag acggcaccgt caacctgacc gcctccgggg cgactccgaa
      121 cgcgacggcc gagctatggg gcctcgagct gtacccgaac acctcgtacc tggtgacgct
      181 ctcgggggcg gtccaggaca tagccggcaa ccccctcggc gcagacaaga cctggtactt
      241 cagcaccggc gacgccgagg acacgagccc gccgaccggc gcggtcacgc tgaccgacca
      301 aggcggcgcg gttatcgtcg tcacgttgcc gtccgggtcg atagccaccg acgacgcgac
      361 gctcgagctg gatttctcgg ccatcacgga cgactacaac atcccctacg gcatcaagat
      421 atggggcgac aacggcaacg ccgctgcctt tttcgaggaa gacgcgagct ggaggacctg
      481 gtccgacacc gtatcgtgga cgctatcggc cggctcggga accaagtacg tgctctacaa
      541 gctgatggac tcggccaaca atcagtccgt cacgccgcgt cagctcagga tcatcctgga
      601 ctccggcgag ctcccgacaa ttgcgactgt ttcggtgaac ggcggtcaga gccatacgaa
      661 cgcttcggac cgggccgtcg accttaccat cgaagcgagc gacgcctata gcggcgtcaa
      721 gcagatgata atctcgcatg accctagctt cgctggcgcc tcgtggatga actatacgcc
      781 gatcttggaa ggctgggtct tgccggacgg cgacggaacc cgcatcttct acgcaaaggt
      841 tcgcgactat ctggatcagg aatcggccac caatcaggcc tgctcgatca cgctggaccg
      901 cgccgcgccg agcgtctcgt tcaacgtcga gagcatcctg gccagcgagc cgaccctgct
      961 cgccgagggc agcgaaggct cagacctcta ccgtatcatc gaggagttcg gcgtggcctc
     1021 gtgcgcgtgg gaacagctga gcggccaagg gacgctgtac ttcaacgccg acgacggcgg
     1081 atccaccgac aacgacggca tagggctcag ggaaccgtac atccacgcga ccgtcgaggg
     1141 ctcgtacttc gcgcgggtca ccgtgaccga taacgcgggc aactcctccc aggcaaccat
     1201 cccattctcc tgggacgtga cgccgcccgc cgaccttgcc gcagtgaacg ttagcgccta
     1261 cgacacgagc gggcagccta cctggtcatg ggccaccgtg gccgacgccg actattaccg
     1321 cgcgagctac tcgtcggatt tctcgagtta tgtcgatgtc cactcgacct cgttcactcc
     1381 gaacgcgccg ctcgctccag acggcgtcaa gacgctctac gtgcgcgccc aggacgaggc
     1441 cggcaactac tcgacgacgc gatcggcctc gacccacgtg gacaccacgg cgccgacgat
     1501 ctcggtcacg accaacagct tcgtggccaa cgtcgccgcc ccgaccgcga ccctcgcctt
     1561 caacggcacc cacggcagcg tggccgactc aggctcgggc gtccagagcg gcctcgacac
     1621 ttcgtcgtac ctgtgggaag aactatccac acccaagtac ctcagcttcg gctcggcctc
     1681 ggctctatcg actacggtga gcgcttcgat cgataagacc taccagattc gattcagggc
     1741 cagggacctg gccggcaacg tgagctacgc gacggtgtcc ctgctcaggg acacgaaccc
     1801 gccagccgcc acggcggtga ccgggccggc tatcaccccg agcctccgac caacctggtc
     1861 ctggagctcg agcagcgggg gcgccggcct cttccagttc tacctgacca acgacagcga
     1921 ctcgaccacg gtctatacaa gcggcgagac caccgtgaag aactaaaagc cggcctcctc
     1981 gctaccggac ttcaagacct ataccatgcg tgtccgcgag cgcgacgggg cgggcaactg
     2041 gtcggcctac gggacgcgca cgaccgtggt cgactcctcg gcgacgacgc cggcccagat
     2101 cgccctgggc gacggttacc cggcgctgcg cacggtcgcc tcggtcgagt gggatctcct
     2161 gaccggttgc ggcggagccg cgaacctcta ccgctacgac gtcgactcca cggggacctg
     2221 gacctacggc ggcgcggcgc tcagcgagaa cgtgccggtc acgatcagcc gatcgggcct
     2281 gaccgagggc gcccatacca tcgtcgtcca ggagcgcatc gacaccgctt ggcgcacaga
     2341 catccaggcg agccacacga tcacggtcga cacggttaag ccgacggagc cgaccctaac
     2401 gggaacgggc ctggctaccg ggcagagcta ccggaccgcg acgccggaca cgacacctac
     2461 ctggatctgg tcgccaggcg gcggcggcgg caacggcgtc tactcctatc gggtcacgcg
     2521 tgtctataca gccgcaggca tcgccgacgg cgcagtgatc gtggcgcaga ccgccgagac
     2581 ggcggcgatg agctacacgc cgtcgtcggc cctggccgag ggcacctatg cgctatccgt
     2641 cagggagcgg gacgaggcgg gcaactggtc gctcgacgcg acccagtaca cgaccgtcga
     2701 caggaccaag ccgacgatgg tcggcgtgct ggtacgcgga ccgacccacc ccgacgacac
     2761 tgact
//
LOCUS       DAOPHV010000365         2757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_51446_length_2757_cov_4.411177, whole genome shotgun sequence.
ACCESSION   DAOPHV010000365 DAOPHV010000000
VERSION     DAOPHV010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51446_length_2757_cov_4.411177"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PLQ29_09480"
     CDS             complement(<1..614)
                     /locus_tag="PLQ29_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86917.1"
                     /translation="MKSPSLATAFDKAIADTARRIDTLLAGNPDQTELEALQTELAAL
                     IRNRRDHLYSLVDARAIVSQVFDEPFSETRRQKAAEIEGQVLLLIEQYHREADETKEV
                     LRSDYDLLVDVLAFLRFFKHDDPPVADTDSPAPRLIWTGKHGLTRQAFVEALQVAKLI
                     TLQNATLLAATLEDYKAEKKAKRPKVAWDGSKESLVTFLIMSYY"
     gene            complement(694..1281)
                     /locus_tag="PLQ29_09485"
     CDS             complement(694..1281)
                     /locus_tag="PLQ29_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86918.1"
                     /translation="MPVSKLRLPRKDLIPPLPDIAELARQLQARLEGLQDPTLRAEEI
                     GITLGELDRIKLHADTLYRRLSSEGDLDEPDAYWKYSEELDRFRHELLTGRDRELLAA
                     RRPEPEQTATRKPRQMPIPAFLAEKDDRQARNRERTDLPDTMNMAQLASYLGVSTQWA
                     YQNYKRLAIPYAVIGCLRRFRKDKVDAWWEAQSRQ"
     gene            complement(1268..2620)
                     /locus_tag="PLQ29_09490"
     CDS             complement(1268..2620)
                     /locus_tag="PLQ29_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPG86919.1"
                     /translation="MTRTTKNRFTQQLFTRPASDGRSIVYVRFIDAESGLVLATRSTS
                     LDSDSPAKDIKASVTSILADLNLEAIARMKDRARRAALDDDERIASMPAFEFFSWFWS
                     DDSYYLSERQDADRPLSKGHILVCRKYVQTNLKQFEAFSLTPVRELRLSIVEAFVRKL
                     RKAGKSLDVVKRNLDTIRTPINWAQARGLIEAPFSFKTLVLPRRDSRERGILSDEEIL
                     KIVDLPYAKPWVAESGRMHWDVRPRPRQSGGRTYDTPPMLDVRQKAAIVMALFAGLRR
                     GEIRGLRWCDIDAERKLIEVRHNYVRFDGDKAPKKMSLGTVPLAVELEQMLDDLRALC
                     ESLGYTGANDFVLPGTARDRPVNEVTIRRGWERALELIGIPERERLKRNLLLHGARHA
                     YTTRLLDSGELSPAEVAKLTRHKDLSMLSRYGGHLRDETIEKGRKALSFKKEADHASQ
                     "
BASE COUNT          483 a          756 c          884 g          634 t
ORIGIN      
        1 aaataatacg acatgatcag aaaggtgact aaggactcct tgctcccatc ccaggccacc
       61 ttcggccgct tggctttctt ctcggccttg tagtcctcga gcgtggctgc cagtaaagtg
      121 gcattttgca gggtgatgag cttggctacc tggagggcct ctacgaaagc ctgtcgcgtc
      181 aggccatgct tgccggtcca gatcaaacgg ggtgctggag aatcagtatc ggcgacaggt
      241 ggatcgtcat gtttgaagaa gcgcaggaag gcaaggacat ctactagtag atcgtaatct
      301 gagcgcaaga cctccttggt ctcatcagct tcccgatggt attgttcgat taacaataag
      361 acctggcctt cgatttcggc cgccttctgg cgacgggttt cagagaatgg ttcgtcgaag
      421 acctggctga cgatggctcg ggcgtcgacg aggctatata ggtgatcccg ccgatttctg
      481 ataagggcgg caagttcggt ctgtaaggcc tctagctcgg tttggtcagg gtttccggcg
      541 agaagcgtat cgattcgacg ggcggtatcg gctatggctt tatcgaaggc cgtggctaag
      601 gatggtgatt tcatatcgac agtttatcat ctgcgatatg atggcgccac tgccttatag
      661 gcatcggcaa aatacctata aggcggtggt cggttactgc ctgctttgag cctcccacca
      721 agcgtcgact ttgtccttgc ggaagcgcct gaggcatccg attaccgcgt agggtatggc
      781 gaggcgtttg tagttctgat aggcccactg ggtgctgact cccaggtagg acgctagttg
      841 tgccatgttc atggtatcgg ggaggtcggt ccgctcccgg ttcctagcct gtcgatcgtc
      901 cttctcggcc aggaaggcgg gtatcggcat ctggcgcggc ttccgggttg cggtttgctc
      961 cggttcgggg cggcgggctg cgagcagctc gcgatcacgt ccggtgagaa gctcatgtcg
     1021 gaaccggtcc aactcctcgg aatacttcca gtatgcgtca ggttcatcca ggtcaccctc
     1081 actgcttaac ctgcggtaaa gggtgtcggc atgcagcttg atgcgatcca gctccccgag
     1141 ggtgatcccg atctcttccg ctcgaagcgt cgggtcttga agtccttcga ggcgtgcttg
     1201 aagctgtcgc gccagttcgg cgatgtccgg gagtggtggg attaggtctt tcctgggtag
     1261 cctgagttta ctgactggca tggtctgcct ccttcttgaa gctgagggcc ttgcgaccct
     1321 tctctatggt ctcgtcgcgg agatggccgc cgtagcggga tagcatggaa agatccttgt
     1381 ggcgggtcag cttggccact tcggccggtg agagctcccc ggaatccagg agccgggtag
     1441 tgtaggcgtg gcgcgcgcca tgaaggagca ggttgcgctt gagccgttcc cgttccggaa
     1501 taccgataag ctccagggcc cgttcccagc ctcggcgaat cgttacttcg ttgacgggtc
     1561 gatcccgcgc ggtgccgggt aggacgaagt cgttggcgcc ggtgtaaccc aggctctcgc
     1621 agagggctct aaggtcgtcg agcatctgtt caagctccac cgccaacggc acggtgccga
     1681 gggacatctt cttcggcgcc ttgtctccgt cgaagcggac atagttgtgt cggacttcga
     1741 tcagcttcct ttcggcatcg atatcgcacc agcggagccc gcgtatctca ccccgcctga
     1801 ggccggcgaa caaggccatc acgatggccg ccttctggcg tacatcgagc atgggcggcg
     1861 tatcgtaggt acgccctccg ctctgccggg gtcgaggccg gacgtcccag tgcatacggc
     1921 cggactcggc gacccacggc ttggcgtagg gtaggtcgac gatcttcaag atttcctcgt
     1981 cgctcaggat gccccgctcg cggctgtccc ttcgaggcag gacgagggtc ttgaaggaga
     2041 acggcgcttc gatgaggcct cgcgcttggg cccagttgat cggcgtccgg atggtatcga
     2101 ggttccgctt gaccacgtcc aggctcttgc ccgccttgcg gagcttccgt acgaaggctt
     2161 ctacgatgga gagcctgagt tcgcggaccg gggtcaggga gaacgcctcg aactgcttca
     2221 ggttggtctg gacgtacttg cggcagacga ggatatgccc tttggagagc ggccggtcgg
     2281 cgtcctggcg ttcggacaag tagtagctgt cgtcgctcca gaaccaggag aagaactcga
     2341 aggcgggcat ggaggcgatc cgctcgtcgt cgtcgagagc ggctcgtcgg gcgcggtctt
     2401 tcatcctggc tatggcttcc aggttgaggt ccgcgaggat gctcgtgacg ctggccttga
     2461 tatccttagc cgggctgtcg gaatcgaggc tggtggagcg cgtcgcgagt acgagtccgc
     2521 tctcggcatc gatgaagcgg acataaacga tagaccggcc atcgctggcc ggtctggtaa
     2581 agagttgttg cgtgaagcgg ttcttggtag ttcgggtcat ggggccactg tactccgtct
     2641 gtttggtaac aaacgcaagt aacagtggcc ttttctacct cacgacctca cgtaattcct
     2701 tgtcgggctg agaggatttg aacctccgac atcttggtcc cgaaccaagc gcgctag
//
LOCUS       DAOPHV010000366         2749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_51628_length_2749_cov_4.710468, whole genome shotgun sequence.
ACCESSION   DAOPHV010000366 DAOPHV010000000
VERSION     DAOPHV010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51628_length_2749_cov_4.710468"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PLQ29_09495"
     CDS             complement(<1..386)
                     /locus_tag="PLQ29_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86920.1"
                     /translation="MSAPFKHRGTGFQARGVAVVAAFAIAALGAGPVAEETLVIDAAS
                     TAPGSPRQWQFDGAIASSGPAGPVLGLSPTPADAGEEPDLRLSFDEAPAHRHARERGA
                     FVDVGGVTQPAPAPRFDRTPAARPRRA"
     gene            480..2360
                     /gene="uvrC"
                     /locus_tag="PLQ29_09500"
     CDS             480..2360
                     /gene="uvrC"
                     /locus_tag="PLQ29_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPG86921.1"
                     /translation="MDENKARPSAGLRNSAKQAPEAPGVYLWKDADGVVIYVGKAKSL
                     KARLASYFTGKREIKTRHLVAKASTLEWILTETEYEALLLENNLIKRYAPKYNINLKD
                     GKTYPSIRITNEEYPRVFRTRRIVDDGSRYFGPYPSADVIDRYLELVRRLFPLRRCKT
                     LRRRETPCMYYHIGRCSAPCAGKTTREEYALQVERVATLLSGDSEALVEALRADMAAA
                     SEALQFEKAAYLRDSVQAIESFRGDSGVVDFAYGDRDYLAWAVDGSLVDFVVFQTRSG
                     RLVGRDLYRERSFGSEAEAASEFMAAYYGPERLPPPEVFVKDAEGLELAADFLRRGLG
                     AAFRLAAPDERRHEAALAMAEHNAREDLAKRRRESGDVEALEALRAALGLEVLPELIE
                     GFDIAQLSGKHTVASLVSFRNGAANKKGYRIYKIKSLDGGIDDFGAMREATARHYTHV
                     ANDEAEAPDLVLIDGGRGQVNAAWEILDALGLDLPVVGLAKENEELWPHGAREPVVLP
                     KDSPALRLVVAVRDEAHRFATGMNQRLRGKALGFPVLEAVKGVGPTRARRIMDAFGSL
                     AAVAEAEPSYIAARSGLDGKTAEAVRDAASRAASAMGAAASSSAAGGTAGLDEVVGPG
                     RS"
     gene            2364..>2749
                     /locus_tag="PLQ29_09505"
     CDS             2364..>2749
                     /locus_tag="PLQ29_09505"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPG86922.1"
                     /translation="MSRRFPFPTTATAALILVAVLLPGSALPEGPGVPGLDKLVHAVL
                     FFGLALAAQLDFGLDGRRRVAVALVAALAFSALTEALQLAVDGRSSELLDMAADMGGL
                     VVGLACRRPLAHAARRAAAIVVSRLSG"
BASE COUNT          405 a          960 c          978 g          406 t
ORIGIN      
        1 gcgcggcggg gccgggccgc cggcgtgcgg tcgaagcgcg gcgcgggcgc cggctgcgtc
       61 acgccgccga cgtcgacgaa ggcgccacgt tcgcgcgcgt ggcggtgcgc cggcgcttcg
      121 tcgaaggaca gccgcaggtc gggctcctcc cccgcgtcgg ccggcgtcgg gctgaggccg
      181 aggaccgggc cggccggccc cgaagaggcg atcgcgccgt caaactgcca ctgtcggggc
      241 gagccgggcg cggtgctcgc cgcgtcgatg acgagcgtct cctccgccac cggacccgcg
      301 ccgagcgcgg ctatggcgaa ggccgccacg acggccacgc ccctcgcctg gaatccggtg
      361 cctctatgct tgaagggtgc gctcatgctg ttcgttcctc gatatcgatg gggaatcggc
      421 cctatggccg gggaggctcc acattcaggc gaaaccccgt tatcatatta tcgaagatta
      481 tggacgagaa taaagcccgg ccttccgccg gattgaggaa tagcgccaaa caagcccccg
      541 aggcgcccgg tgtctacctc tggaaggacg ccgacggcgt cgtcatctac gtcggcaagg
      601 ccaagtcgct caaggcgagg ctcgcctcgt acttcaccgg caagcgcgag atcaagacga
      661 ggcacctcgt cgccaaggcc tcgactctcg agtggatact cacggagacc gagtacgagg
      721 ccctgttgct cgagaacaac ctgatcaagc gctacgcgcc caagtacaac atcaacctga
      781 aggacggcaa gacctatccg tcgatacgca tcaccaacga ggagtacccg agggtcttca
      841 ggacgaggcg catcgtcgac gatggtagcc gctacttcgg gccctacccg agcgccgacg
      901 tcatcgaccg ctacctggag ttggtcaggc gcctgttccc gctccgtcgc tgcaagacgc
      961 tcaggcggcg cgagacgccg tgcatgtact accatatcgg ccgttgctcc gccccctgcg
     1021 ccggcaagac gacccgcgag gagtacgccc tgcaggtcga gcgggtagcg acgctcctgt
     1081 ccggggacag cgaggctctg gtcgaggcgc tcagggccga catggccgcc gcgtccgagg
     1141 cgctacagtt cgagaaggcc gcctacctgc gcgactcggt ccaggcgatc gagtcgttcc
     1201 ggggcgactc gggcgtcgtc gacttcgcat acggcgaccg cgactacctg gcttgggcgg
     1261 tcgacggtag cctcgtcgac ttcgtcgtgt tccagactcg ctcggggcgc ctcgtcggcc
     1321 gcgacctgta ccgcgagcgt agcttcggct ccgaggccga ggccgcttcc gagttcatgg
     1381 cggcttacta cggccccgag cgcctgccgc cgcccgaggt cttcgtcaag gacgccgagg
     1441 gcctggagct ggccgccgat ttccttcgtc gcggcctcgg cgccgcgttc aggctggccg
     1501 cgccggacga gcgccgccac gaggccgccc tcgctatggc cgagcacaac gcccgcgagg
     1561 acctggccaa gcggcggcgc gagtcgggcg acgtggaggc cctcgaggcc ctacgcgccg
     1621 ccctcggcct cgaggtgctg cccgagctga tcgagggctt cgacatagcc cagctgtcgg
     1681 gtaagcacac ggtcgcctcg ctggtgtcgt tcaggaacgg cgccgccaac aagaaaggct
     1741 accgtatcta taagatcaag agcctcgatg gcggcatcga cgacttcggg gcgatgcgcg
     1801 aggcgacggc ccggcactac acccacgtgg ccaacgacga ggccgaggcg cccgacctgg
     1861 tgctcataga cggcggccgc ggccaggtga acgcggcttg ggagatactc gacgcgctcg
     1921 gcctggatct gccggtcgtc ggcctggcca aggagaacga ggagctgtgg ccccacggcg
     1981 ccagggaacc ggtcgtgctg cccaaggact cgccggcgct caggctcgtg gtcgcggtgc
     2041 gcgacgaggc gcatcgcttc gcgaccggta tgaaccagcg gctgcgcggc aaggcgctag
     2101 gcttcccggt gctggaggcg gtcaagggcg tcgggccgac gagggcgcgg cgtatcatgg
     2161 acgccttcgg ctcgctggcc gccgtggccg aggccgagcc ttcgtacatc gccgcccgtt
     2221 cggggctcga cggcaagacc gccgaggccg tgcgggacgc ggcgtcccgg gcggcttcgg
     2281 cgatgggcgc cgcggcgtcc tcgtcggcgg ccggcgggac cgccgggctg gacgaggtcg
     2341 tcggtcccgg gcgatcatag gccatgtcca ggcgattccc gttcccgacc accgcgaccg
     2401 cggccctgat actcgtcgcc gtgctgctgc ccggctcggc cctgcccgag ggccccggcg
     2461 tgcccggcct cgacaagctc gtgcacgcgg tcctgttctt cgggctggcg ctagcggccc
     2521 agctcgactt cggcctcgat ggccgacgac gggtagcggt ggcgctggtc gccgcgctgg
     2581 ccttctcggc cctgaccgag gcgctccagc tggccgtcga cggccgctcg tcggagctgc
     2641 tcgacatggc ggcagatatg ggcggcttgg tcgtcgggct ggcctgcagg cggccgctgg
     2701 cgcacgcggc acgccgggcg gcggccatcg tcgtctcaag actgtcggg
//
LOCUS       DAOPHV010000367         2749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_51633_length_2749_cov_3.718263, whole genome shotgun sequence.
ACCESSION   DAOPHV010000367 DAOPHV010000000
VERSION     DAOPHV010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51633_length_2749_cov_3.718263"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PLQ29_09510"
     CDS             complement(<1..699)
                     /locus_tag="PLQ29_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86923.1"
                     /translation="MNDNPLLSTWTTPHGLPPFDAIRPEHYAPAFEAAMAEHRAEVDA
                     IAADPAEPTFENTVAALDRAGQGFARVAGVFFNLTASETSDALQAVERELMPKIAAHQ
                     SWLSLHAGVFKRVDALYKKRESLGLAPEQLRLLERVRLDYVMEGALLKGAARKRYAAI
                     AEELAGLFTTFSQWVLADEAAFCLELKTDDDRAGLLVTKTPLFAGEGAGMFVASGGGN
                     IFRAHDKRTGDILWE"
     gene            complement(850..2001)
                     /locus_tag="PLQ29_09515"
     CDS             complement(850..2001)
                     /locus_tag="PLQ29_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86924.1"
                     /translation="MAKTEWIRRVPAIRLSARRGRTGPSLRCLALAAACAFAACQDPD
                     GAGSAPSVYAGGYAKDGSSVSVAGYWNGLTWHPLPNPFGEHHSFVNCLEVSDGVVFAG
                     GSVLDDAGTYYPGYWAYGTLGLLPKPGDHSNNDIVSAIAVSDGEVIAGGYSLDLGASV
                     AIAGYWRNGIWTEAVNGAGEYDTHVDALVVSDGVVYAGGYAVDASDVAVAGYWADGTW
                     HALANPAGAGYGALVFALAISDGHAYAGGLAMNASNVAVAGYWVDGTWTALANPAGAG
                     RMAMVTSMAVSDGHVYAGGYAADGSNVAVAGYWVDGTWTALANPAGAGYSATVKALAV
                     YDGEVYAGGYAMDASSVSVAGYWADGVWTALANPAGAAYDAVVSSLVVQ"
     gene            complement(2173..2436)
                     /locus_tag="PLQ29_09520"
     CDS             complement(2173..2436)
                     /locus_tag="PLQ29_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86925.1"
                     /translation="MVSVPFAYDLDHDGVRLEAVVTQVSPSDVTVELVSPFPGVRYGL
                     HVPYFAMYERNRNLDPDGSVSAKGWGTIRACAIGAYERGLGSA"
BASE COUNT          446 a          946 c          930 g          427 t
ORIGIN      
        1 ttcccacagg atgtcgccgg tgcgcttgtc gtgcgcgcgg aagatgttgc cgccgcccga
       61 tgcgacgaac atgccggcgc cttcgcccgc gaacagcggc gtcttggtga cgagcaggcc
      121 ggcgcggtcg tcgtcggtct tgagctcgag gcagaaggcc gcctcgtcgg cgagcaccca
      181 ctggctgaac gtggtgaaca ggccggccag ctcctcggcg atggccgcgt agcgcttgcg
      241 cgcggcgcct ttgagcaagg cgccttccat cacgtaatcc aggcgcaccc gctcgagcag
      301 gcgcagctgc tccggggcga gcccgagcga ttcgcgcttc ttatacagcg cgtcgacccg
      361 cttgaagacg cccgcatgca ggctcagcca gctctggtgg gcggctatct tgggcatcag
      421 ctcgcgctcg accgcctgga gcgcgtcgct cgtctcgctc gcggtcaggt tgaagaacac
      481 gcccgccacc cgagcgaagc cctgccccgc ccggtccagg gcggccacgg tgttctcgaa
      541 ggtcggctcg gccggatcgg ccgctatggc gtctacctcg gcccggtgct ccgccatcgc
      601 cgcctcgaag gcgggcgcgt agtgctcggg cctgatggcg tcgaacggcg gaaggccgtg
      661 cggcgtcgtc catgtagaaa gcagtgggtt gtcgttcatg ggtagcgatt atcggggcct
      721 tacgtcgggc cgtcaagcga ccgaacggat ataggcgtat agatacaaag ggagagcgcg
      781 gggcctcccg gccccgcgcg acgatatacc gctaccgaag gcgccgttcg gcgcccggcc
      841 tccggccgcc tactggacta ccagcgagct gacgacggcg tcgtacgcag ccccggcggg
      901 attggccagc gcggtccaga ccccatcggc ccaatagccc gcgaccgaga cggaggaggc
      961 gtccatcgcg tacccgccgg cgtatacctc gccgtcgtat accgcgagcg ccttgaccgt
     1021 agcggaatag ccggcgccgg cgggattggc tagagccgtc caggtcccgt cgacccagta
     1081 gcccgcgacc gcgacgttcg atccgtcggc cgcgtatccc ccggcgtaca cgtggccgtc
     1141 ggataccgcc atggaggtaa ccatggccat gcggcccgcc ccggcgggat tggccagcgc
     1201 cgtccaggtc ccgtcgaccc agtagcccgc gaccgctacg ttcgaggcgt tcatcgccag
     1261 ccccccggcg tacgcgtggc cgtcggatat cgccagggcg aataccaggg cgccgtagcc
     1321 ggctcccgcc ggattggcca gcgcgtgcca ggtcccgtcg gcccagtagc cggcgaccgc
     1381 gacgtcggag gcgtcgaccg cgtatccccc ggcgtacacg acgccgtcgg acactaccag
     1441 ggcgtccacg tgggtatcgt actcccccgc gccgttgacc gcctcggtcc agatcccgtt
     1501 acgccagtag cccgcgatcg cgacgctggc accgaggtcg agggagtatc ccccggcgat
     1561 tacctcgccg tcggataccg ctatggcgct gactatgtcg ttattcgagt gatcgccggg
     1621 cttgggcagc aagcccaggg tcccgtaggc ccaatatccc ggatagtagg tcccggcgtc
     1681 gtctagtacg gatcccccgg cgaacacgac gccgtcggat acctccaggc agttgacgaa
     1741 cgagtgatgc tccccgaagg ggttcggcag cggatgccag gtcaggccgt tccagtagcc
     1801 ggcgacggag acggacgagc cgtccttcgc gtacccgccg gcgtacacgc tcggcgcgga
     1861 accggctccg tccgggtcct gacaggccgc gaaggcgcag gcggccgcca gcgccaggca
     1921 gcggagcgac ggacccgtcc tcccgcggcg ggcggagagg cggatcgccg gcacgcgacg
     1981 gatccattcg gtttttgcca tggttatctc cattccggaa cgacagggac tacggcatta
     2041 acggaagtat acttcgaaat cggaagtcca caagcgaata gtaatcgacg ggatcgccga
     2101 cgcggaagcg acggcggatc cggcgacggg ccccgcgcct gtacctgtcg tcgcctccgt
     2161 cctaggtcgg ggttaggcgg aaccgaggcc ccgctcgtac gcccctatcg cgcaggcgcg
     2221 gatcgtcccc catcccttcg cggagacgct tccgtcaggg tccaggttgc gattcctctc
     2281 gtacatcgcg aagtacggca cgtgcagccc gtagcggacg ccggggaacg gcgagacgag
     2341 ctccacggtc acgtcggacg ggctgacctg cgtgacgacg gcttctaggc ggactccgtc
     2401 gtgatcgagg tcgtacgcga acggcacgga taccaaggcg agcctcctct ttagcggtta
     2461 tttctagagc gccccgcaag cgggagcaaa ccggcgctat agcgatacta cgacgcgtcc
     2521 cggagccggt caaggacctg gcgcggggcc gtcggccccg cgcgacgata cgccgctacc
     2581 gaaggcgccg tccggcgccc ggcctgcggc cgccgagcct agggatggtc ggcgctcgct
     2641 cggttcaaat ccggggcgag caaaaaaagt gaccgtcctg tggacggtca ctcatcaata
     2701 gggtatcgct ttagttttct tggtcgggca acccggattt gaaccgggg
//
LOCUS       DAOPHV010000368         2743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_51796_length_2743_cov_3.608631, whole genome shotgun sequence.
ACCESSION   DAOPHV010000368 DAOPHV010000000
VERSION     DAOPHV010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51796_length_2743_cov_3.608631"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PLQ29_09525"
     CDS             <1..733
                     /locus_tag="PLQ29_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86926.1"
                     /translation="LPPERVPTLLKSLSVYWVSNANPDSGNNVKILCFMLGLAHLSIA
                     HLMNIRRDFPSLKFIGQLGQFAMVAGMFFVVLNLVISSTQHPIPTWSLVSIGAGFVLN
                     FMFANYEGGGNFFSALGKSVLASMANIVSVFLGVVNIFADIVSYIRLWAVGLAGLAIS
                     QTVNNMAGPMFGKAIMFAAGIALLVFGHGLNIIMSVLSVIVHGVRLNVLEFSGHLGME
                     WSGYKYEPFRDTVQAERTDMERSIS"
     gene            730..1152
                     /locus_tag="PLQ29_09530"
     CDS             730..1152
                     /locus_tag="PLQ29_09530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525271.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HPG86927.1"
                     /translation="MNMGMIGVAAVLGIAAIGSAIGIGIAGQAAIGAWKRNYMANKPA
                     SFLLVVFAGAPLTQTIYGFILMQRMAVSTKDPALLLGAGVIGGLAMALSAIAQGQAGA
                     ASSDAFGETGKGFGQYITVVGLCETVALFVMAFTFGAI"
     gene            1288..>1709
                     /locus_tag="PLQ29_09535"
     CDS             1288..>1709
                     /locus_tag="PLQ29_09535"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPG86928.1"
                     /translation="MVSKTRPVKVGRVIIGGGHRPSVQTMWKAPLRSGGLESVADGVR
                     GLEALGCDILRFSTPDMEQAELLGALASRVDLPLVADIHFDWRIALRCLDFPIAKIRV
                     NPGNLGEPWKLREVAAKAADKGVPIRIGVNAGSLPADLR"
     assembly_gap    1710..1719
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1720..2348
                     /locus_tag="PLQ29_09540"
     CDS             <1720..2348
                     /locus_tag="PLQ29_09540"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPG86929.1"
                     /translation="RDEPDRGTAIVAAAEREVAALDELGFRDVIVSMKASGVEETVRA
                     NRLFSSKHDIPLHIGVTEAGPLVQGCVRSAAAIYPLLLDGVGDTIRVSLSADPSNEVL
                     AGVELVALATGSRRSADIVSCPRCGRAGFDTHAFTERWCERIYAIRKPVTVAVMGCVV
                     NGPGEARHADIGITGAGDKVMVFRKGEITRTCAPEDADTVFGEELARL"
     gene            2345..>2743
                     /locus_tag="PLQ29_09545"
     CDS             2345..>2743
                     /locus_tag="PLQ29_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86930.1"
                     /translation="MTRGLRVAVAALLAVIASAAPIVAQSSQPAWLSLELGKRAYADK
                     DFGAALVHFDRALMIRRDAFTSAAVRLDQGLDSKAAREAGDSIEAVLSSLAREDFIQR
                     DYARLAAGRRPGDSGLLEDLRRERISDSHRA"
BASE COUNT          454 a          870 c          898 g          511 t
ORIGIN      
        1 cctgccgccc gagcgcgtgc cgacccttct caagtcgctg tcggtgtact gggtatcgaa
       61 cgccaatccc gattcgggta acaacgtcaa gatactctgc ttcatgctcg gcctggcgca
      121 cctgtccatc gcccacctga tgaacatccg ccgcgacttc ccgtcgctca agttcatcgg
      181 gcagctcggt cagttcgcga tggtcgccgg catgttcttc gtcgtgctca acctcgtgat
      241 cagctccacg caacacccga taccgacctg gtcgctggtt tcgatagggg cgggcttcgt
      301 cctcaacttc atgttcgcca actacgaggg cggcggcaac ttcttctcgg cgctcggcaa
      361 gagcgtgctc gccagcatgg cgaacatcgt ctcggtgttc ctcggcgtcg tcaacatctt
      421 cgccgacatc gtctcgtata tccgcctgtg ggcagtaggc ctcgcggggt tagccataag
      481 ccagacggtc aataatatgg ctggaccgat gttcggcaag gctataatgt tcgcggccgg
      541 catagccctg ctcgtgttcg ggcatggcct caatatcatc atgagcgtgc tgtccgtcat
      601 cgtgcacggc gtacggctca acgtactgga gttctcgggc cacctcggca tggagtggtc
      661 cggctataaa tacgaaccgt tcagggatac cgttcaggca gaacggaccg acatggaaag
      721 gagcatctca tgaatatggg catgatcggc gtcgcggccg ttctagggat cgcggccata
      781 gggtcggcga tcggcatcgg catcgccggg caggcggcta tcggggcctg gaagcgcaat
      841 tacatggcca acaagccggc gtcgttcctc ctcgtggtgt tcgccggagc gccgcttacg
      901 cagacgatct acggcttcat cctgatgcag cgcatggccg tctcgacgaa ggaccccgcg
      961 cttctcctcg gcgccggcgt gatcggcggc ctggcgatgg cgctgtccgc gatcgcccag
     1021 ggacaggccg gcgcggcctc ctcggacgcg ttcggcgaga cgggcaaggg cttcggccag
     1081 tacattaccg tcgtcggcct ttgcgagacg gtggcgctgt tcgtgatggc cttcaccttc
     1141 ggcgctatct aggccgtagc gattaggcct tcgggactgg agggggcgcg agcccactcc
     1201 agtctttttt taggcagagc gtctggatcg gctcctgtcc gactgaggcg ctcccatttc
     1261 gttccgcttc cgtatataat ggctcccatg gtaagcaaga caaggcccgt caaggtcggg
     1321 cgcgtcataa ttggcggcgg acaccggccg tcggtgcaga cgatgtggaa ggcgcccctg
     1381 cgctcgggag ggctcgagtc ggtcgccgac ggcgtccgcg gtctcgaggc cctcggttgc
     1441 gatatcctgc ggttctctac gccggacatg gagcaagcgg agctactcgg cgccctcgcg
     1501 tcgagggtcg acctgccgct cgtcgccgac atacacttcg attggcgtat cgcgctgcgc
     1561 tgtctcgatt ttcccatagc caagatacgc gtcaacccgg gaaacctggg cgagccgtgg
     1621 aagcttcgcg aggtggccgc gaaggcagcc gacaagggcg tgccgatacg gataggcgtc
     1681 aacgcgggca gcctgccggc cgacctgcgn nnnnnnnnnt gcgggacgag cctgaccgcg
     1741 gtacggcgat agtcgccgcg gccgagcgcg aggtcgccgc tctcgacgag ctgggcttcc
     1801 gggacgtgat cgtatcgatg aaggcctccg gcgtcgagga aacggtgcgc gccaaccgcc
     1861 tgttctcgtc gaagcacgat ataccgctcc atataggcgt taccgaggcc ggtcccctcg
     1921 tccagggctg cgtgcgtagc gccgcggcga tatacccgct gctgctcgac ggcgtcggag
     1981 acacgatacg cgtctcgcta tcagccgacc cctcgaacga ggtgctggcc ggcgtcgagc
     2041 tggtagcgct ggcgaccggc tcgcgcagga gcgccgacat cgtatcctgt cctcgctgtg
     2101 gacgggccgg cttcgacacg cacgcgttca cggagcgctg gtgcgagcgt atctacgcga
     2161 tacggaagcc ggtcacggtc gcggtcatgg gctgcgtcgt caacggcccg ggagaagcca
     2221 ggcacgcgga tataggcatt accggggcgg gtgacaaggt gatggtattc cgcaagggcg
     2281 aaataacgcg aacgtgcgcg cccgaagacg ccgatactgt attcggggag gaactcgccc
     2341 gattatgacc agaggattac gcgtcgccgt ggcggcgctt ctagccgtca tcgcgtcggc
     2401 ggccccgatc gtcgctcagt cgtcccaacc ggcgtggctg agcctggagc tgggtaagcg
     2461 ggcgtacgcc gacaaggact tcggggcggc cctcgtgcac ttcgaccgcg cgctgatgat
     2521 ccggcgcgac gcgttcacgt cggcggccgt acgcctcgac caggggctcg attccaaggc
     2581 ggctcgagag gccggcgact ccatcgaggc ggtattgtcg tcgttggctc gcgaggactt
     2641 catccaacga gactacgctc gactcgccgc cgggcgcagg cctggcgata gcggcctcct
     2701 cgaggacctg cgacgtgaac gcatatccga tagccaccgc gcc
//
LOCUS       DAOPHV010000369         2743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_51798_length_2743_cov_3.402158, whole genome shotgun sequence.
ACCESSION   DAOPHV010000369 DAOPHV010000000
VERSION     DAOPHV010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51798_length_2743_cov_3.402158"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..2422)
                     /locus_tag="PLQ29_09550"
     CDS             complement(32..2422)
                     /locus_tag="PLQ29_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPG86931.1"
                     /translation="MEQKDYRLAAIMYTDIVGYSRMMERDEAGTLSLLHYHNALVSDI
                     AAKRHGTVIKTIGDAFLIDFRNTVEALQCAMEVQSAIYERNKQTDSQPLLLRIGLHLG
                     DIYFFENDALGEGINIASRLQSLARPGCVCFSQDVYNQVLNKIDFQAEKLGKVSLKNI
                     TKEIHAYEIASPNVEFYPDRDKPRVGFKPVAEESPAATKPLPGAPTPSDRGYGPEAAR
                     GVLDDIRKAILADTKTMGRRLTVAEALERYGEYGVEAKEVIATMAEKGLIQKSPATSG
                     AAGTGAQGGSDLAADIGKAVEGIARAIEQSVGDWQKAGRSGRSSRGDWSDKDGWSDVN
                     GRDRESRYADIGRRVERGAERMVDTIERELGRSSILDEKSAIKAEFKRHEAEVATGKW
                     DSELRSSDHFKPSSEELETDFSRYRDKIESKAHKSSGGFVGNLLSFVGVNALLWYINL
                     KVAPGFMWAAIVSAGWGTGIVSGFFAMLRGKAKASEVDKMPELEPGALDVYKKVNRVK
                     DSMAMHLASILSVPPLLFTINLLTSPGFLWAVIPSGIMALSFIGHLIAYPGTLRGLEK
                     RLFRMLGVDSWRELFRLGKGRRAGAAAGPYASVYAEAASIRDEIVKELKAGKGSDEFG
                     KDMIPTLDRYVEQVKLLSHSVNDIDGIVSTIPTEALRRDREALETKRESAGSQALKDE
                     YGRSIDEIARQEKACRDLEDQRELLRLRLGSSVNALKQFKLDLARMKALPEAGERQAM
                     EDLRRKAGELSGYLDDLKKGYDESSGDPYAELERLVAESEAKARAEGAEGLGGPGR"
     gene            complement(2469..>2743)
                     /locus_tag="PLQ29_09555"
     CDS             complement(2469..>2743)
                     /locus_tag="PLQ29_09555"
                     /inference="COORDINATES: protein motif:HMM:NF037864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HPG86932.1"
                     /translation="LARAAAGAQAVRKAERQSELHWTLAWLLERPGWEGEAVVVQTGN
                     GDALLYLPEIGLETRVRASGHELNTALRVRFQQADISRLEARFSVI"
BASE COUNT          445 a          899 c          853 g          546 t
ORIGIN      
        1 ggccaatact ataaagaatg gcgggtgcct gtcaacgacc cggtccaccc agcccctcgg
       61 caccctcggc gcgagccttg gcctcggatt cggccaccag ccgctccagc tcggcgtagg
      121 ggtctccgga actctcgtcg tagcccttct tgaggtcgtc gagatagcct gacagctcgc
      181 cggccttgcg gcggaggtcc tccatggcct ggcgttcgcc cgcctcgggc agggccttca
      241 tcctggctaa gtcgagcttg aactgcttga gcgcgttgac cgacgagccg agcctgaggc
      301 gcaggagctc gcgctggtct tccaggtcgc ggcaagcctt ctcctgcctg gctatctcgt
      361 cgatcgagcg accgtattca tccttgagcg cctggctacc ggctgactcg cgcttggtct
      421 cgagcgcctc cctatcgcga cggagcgcct cggtcgggat ggttgaaacg ataccgtcga
      481 tatcgttgac cgaatgcgac agcagcttga cctgctcgac gtagcgatcc agggtcggga
      541 tcatgtcctt accgaactcg tccgagccct tgcccgcctt gagttccttg acgatctcgt
      601 cgcgtatcga ggccgcctcg gcgtagaccg acgcataggg gccggccgcc gctcccgccc
      661 tgcgaccttt gccgagcctg aacagttcgc gccacgagtc gacgccgagc atgcggaaga
      721 gccgcttctc gaggccccgg agcgtgccgg ggtaggcgat gaggtggccg atgaagctaa
      781 gcgccataat accagacggg atgaccgccc ataggaaccc gggcgaggtt agcaggttga
      841 tcgtgaacag gagcggcggc accgagagga tgctcgcgag gtgcatcgcc atgctgtcct
      901 tgacgcgatt caccttctta taaacgtcga gcgcccccgg ctcgagctcc ggcatcttgt
      961 cgacctcgga ggccttggcc ttgcccctga gcatcgcgaa gaagcccgag acgatgcccg
     1021 tgccccagcc cgcgctgacg atggccgccc acatgaaacc gggagcgacc ttcaggttga
     1081 tataccagag cagggcattg acgccgacga acgagagcag gttgccgacg aacccgcccg
     1141 aagacttgtg ggccttggac tcgatcttat cgcggtagcg ggagaagtcg gtctcgagct
     1201 cctcggaact cggcttgaag tggtcggagg agcggagctc cgagtcccac ttgccggtgg
     1261 cgacctcggc ctcgtggcgc ttgaactcgg ccttgatagc gctcttctcg tcgagtatcg
     1321 acgagcggcc tagctcgcgc tcgatcgtat cgaccatgcg ctccgcgccg cgttcgaccc
     1381 gacggcctat gtcggcgtac ctggattcgc gatcccgacc gttcacgtcc gaccagccgt
     1441 ccttgtccga ccagtcgccg cgactggagc gcccgctcct gccggccttc tgccagtcgc
     1501 cgacgctctg ctcgatggcg cgcgctatgc cctcgaccgc cttgccgatg tcggcggcca
     1561 ggtcggagcc accctgagct ccagtgcccg ccgctccgct cgtcgccggg gacttctgga
     1621 tgagcccctt ctcagccatc gtcgctataa cttctttggc ctcgacacca tactccccgt
     1681 agcgctcgag agcctcggcg acggtcagcc tgcgtcccat agtcttggtg tcggcgagga
     1741 tggccttgcg tatgtcgtcg aggacgccgc gtgccgcctc ggggccgtag ccgcggtctg
     1801 acggagtcgg cgcgccaggt aagggtttcg tggcggccgg cgattcctcg gcgaccggct
     1861 taaaaccgac gcgcggcttg tccctgtccg ggtagaattc gacgttagga ctcgcgatct
     1921 cgtaggcgtg gatctcctta gtgatattct tgagcgacac cttaccgagc ttctccgcct
     1981 ggaagtctat tttgttgagc acctggttat acacatcctg ggagaagcat acgcaccccg
     2041 ggcgggcgag cgactgcagg cgactggcta tgttgatgcc ttcgccgagg gcgtcgttct
     2101 cgaagaagta gatgtcgccg agatgtaggc ctatacggag tagcaacggc tgcgaatcgg
     2161 tctgcttgtt gcgttcgtag atggccgact gcacctccat cgcgcattgt agcgcttcca
     2221 cggtattgcg gaagtcgatc aggaaggcgt cgccgatggt cttgatgacg gtgccatgac
     2281 gcttggcggc tatgtccgat acgagggcgt tatggtagtg gagcaatgat agcgtgccgg
     2341 cttcgtcccg ctccatcatc ctcgagtatc cgacgatgtc ggtatacatg atcgccgcga
     2401 ggcgatagtc tttttgttcc atgctcaccg cgctagcata gaatagccgt agccgcgggt
     2461 caactgcatc atatgacgct gaagcgggcc tcgagcctcg aaatatcggc ctgctggaag
     2521 cgtacgcgta gagccgtatt caactcgtgc cccgaggcgc gcaccctcgt ctcgagacct
     2581 atctccggca ggtacaggag cgcgtcgccg ttcccggtct ggacgacgac cgcctcgcct
     2641 tcccagcccg gtcgctcgag tagccaagct agcgtccagt gcagttcgct ctgccgctcc
     2701 gccttgcgga ccgcctgcgc tccggccgcc gccctggcca ggc
//
LOCUS       DAOPHV010000370         2729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_52172_length_2729_cov_3.194465, whole genome shotgun sequence.
ACCESSION   DAOPHV010000370 DAOPHV010000000
VERSION     DAOPHV010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52172_length_2729_cov_3.194465"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1988)
                     /gene="lon"
                     /locus_tag="PLQ29_09560"
     CDS             complement(<1..1988)
                     /gene="lon"
                     /locus_tag="PLQ29_09560"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPG86933.1"
                     /translation="MSMLESLRPKGGALEIPLVYVRDAVVFPHTIAPVLAATKFCIAA
                     SEVAAKSDKIIFISLLRSLPVDGANDIDVHEIGTVAHVIQSIKLADGSVRLLVDGLRR
                     GRIRRTVFRKEYLGAIVEAIDDGATGDDGATGDDGATGDDGGPSGADGRKELEALVQI
                     LRRDFSTYADLVKKIPNETMLAVQRAESPHKLCGTIANAMGLKLERKQELLAVVPLRE
                     RLEATAKALSQDIELLSIQKRISQKVRARMEKSQKDYFLQEQLKEIHKELGRDDDESD
                     LAELTRRIESKAPPQEVLDKAKKELARLAKLQPLSPEAGVIRGYCEWLADLPWSARKA
                     DSKDLAAAKAALDEDHYGMRKAKERILEFIAVRQLNDSLKGPILCLVGPPGTGKTSLG
                     RSVARALGRDFVRMSLGGVRDEAEIRGHRKTYVGALPGKILQSMRKAGSSNPVFLLDE
                     IDKMSSDFRGDPASALLEVLDPEQNASFVDHYLEVPYDLSGVMFVTTANSVHSIPYAL
                     LDRMELIEIPGYSEYEKLEIARHFIVPRQIRENGLEGSSTRIRDDAVLEIIRHYTMES
                     GVRSLEREIARVVRKTAERAVEAGYAGGQRPLAEFKRVIGASSLRKLLGKRRHENDLV
                     FSEPGPGLACGLAWTEMGGSVLPVEASSFEGEGELIMTG"
     gene            complement(1985..>2729)
                     /gene="clpX"
                     /locus_tag="PLQ29_09565"
     CDS             complement(1985..>2729)
                     /gene="clpX"
                     /locus_tag="PLQ29_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPG86934.1"
                     /translation="GGNVSLAEKGIIYIDEIDKIARKTQNVSITRDVSGEGVQQALLK
                     IIEGTVANVPPQGGRKHPQQEYIRIDTTNILFICGGAFVGLDKTIEARVAKAALGFGA
                     ELQEKTKIGLRELFMNMHPDDLVKYGMIPEFIGRLPIQVALDNLEAEDLKRIITEPKN
                     SILRQYQESFKLDGAELVFAPEAIDAIAEQAIARKTGARGLRSIVENLLIDVMYDLPS
                     GSENRRVIVTRDTVVLKSPPVIEGLKKSA"
BASE COUNT          417 a          853 c          886 g          573 t
ORIGIN      
        1 cccgtcatga tcagctcgcc ctccccctcg aagctcgagg cctcgacggg cagcaccgag
       61 ccgcccatct ccgtccaggc gagcccgcag gccaggccgg gaccgggctc ggagaagacc
      121 aggtcgttct cgtggcggcg cttcccgagc agcttgcgta gcgacgaggc gccgatcacg
      181 cgcttgaact ctgcgagggg cctctgcccg ccggcgtaac ccgcctcgac ggcccgctcc
      241 gcagtctttc tgacgactct ggctatctcc cgctcgaggg agcgcacccc ggattccatc
      301 gtgtaatggc ggatgatctc gagcaccgcg tcgtcgcgta tcctcgtcga cgagccttcg
      361 aggccgttct ccctgatctg cctcgggacg atgaagtgcc tggctatctc gagcttctcg
      421 tactcgctat agccgggaat ctctatcagc tccatacggt cgagcagggc gtagggtatc
      481 gagtggacgc tgttggcggt ggtgacgaac atgacccccg acaggtcgta cggcacctcg
      541 aggtagtggt cgacaaagct ggcgttctgc tccgggtcga gtacctcgag cagcgcgctc
      601 gccgggtcgc cccggaagtc gctcgacatc ttgtcgatct cgtcgaggag gaagaccggg
      661 ttcgacgagc cggccttgcg catgctctgg agtatcttac cgggtagcgc gcccacgtag
      721 gtcttacggt ggccgcgtat ctcggcctcg tcgcgcacgc cgccgaggct catgcgcacg
      781 aagtcgcggc cgagcgctct tgcgacggat ctgccgaggc tagtcttgcc ggtgcccggc
      841 gggccgacga ggcagagtat cgggcccttg agcgaatcgt tgagctgcct gacggctatg
      901 aactcgagga tgcgctcctt ggccttcctc atgccgtaat gatcctcgtc gagcgcggcc
      961 ttggccgccg ccaggtcctt ggagtcagcc ttgcgcgccg accagggcag gtcggccagc
     1021 cactcgcagt agccgcggat gacgcccgcc tcgggcgaca ggggctgtag tttagctagc
     1081 ctggccagct ctttcttggc cttgtccaga acttcctgag gtggcgcctt cgactcgatt
     1141 cggcgggtca gttcggccag gtccgactca tcgtcgtcgc gccccagttc cttgtggatt
     1201 tccttgagct gttcctggag gaagtagtcc ttttggctct tttccatcct ggcgcggacc
     1261 ttctgggaga tgcgcttctg gatcgacaag agctctatgt cctgggaaag ggccttggcg
     1321 gtagcctcga ggcgctcgcg aagcggtacc acggccagga gctcttgctt ccgctccagc
     1381 ttgagcccca tggcgttggc tatcgttccg catagcttat ggggagactc ggcgcgctgt
     1441 acggccagca tggtctcgtt cggtatcttc ttgacgaggt cggcgtaggt cgagaagtcg
     1501 cggcgtagga tctggacgag ggcttcgagc tccttacgcc catcggcgcc gctcggtcca
     1561 ccgtcgtcgc cggtagcgcc gtcgtcgccg gtggcgccgt cgtcgccggt ggcgccgtcg
     1621 tcgattgcct cgacgatggc accgaggtat tccttgcgga acacggtgcg cctaatgcgc
     1681 ccgcgccgta gcccatcgac gagtagacgg acggagccgt cggccagctt gatcgactgg
     1741 atcacgtggg cgaccgtgcc tatctcatgc acgtcgatat cgttggcgcc gtcgacgggg
     1801 aggctcctga ggagggaaat gaagatgatc ttgtcggact tggccgccac ctcggacgcg
     1861 gcgatgcaga acttggtggc ggctagcacc ggcgcgatcg tgtgcgggaa cacgaccgcg
     1921 tcgcggacat atacgagtgg aatttccaat gcgccaccct tggggcgcag ggattccagc
     1981 atgctcatgc gctcttcttc agtccttcga tgacgggggg cgacttcaat accacggtgt
     2041 cccgtgtcac gatgacgcgg cggttctcgg acccgctcgg aaggtcgtac atcacgtcga
     2101 taagaagatt ttcgacgatt gatctgagcc cgcgggcgcc ggtcttgcgg gcaatggcct
     2161 gctcggctat cgcgtctatc gcctccgggg cgaacacgag ctcggcgccg tcgagcttga
     2221 agctctcctg gtactgcctg agtatcgagt tcttaggctc ggtgatgatg cgcttgagat
     2281 cctcggcctc gaggttgtcg agggccacct ggatgggcag gcgcccgata aactcgggta
     2341 tcatgccgta tttgaccagg tcgtccgggt gcatgttcat gaacagctcg cggaggccta
     2401 tcttggtctt ttcctgcagc tcggcgccga agccgagagc cgctttggcg actcgggcct
     2461 cgatggtctt gtccaacccg acgaaggcgc ctccgcagat gaacaggatg ttcgttgtgt
     2521 cgatacggat atactcctgc tgcgggtgct tgcggccgcc ctgcggaggt acgttggcca
     2581 cggtgccttc gatgatcttg agcagggcct gctgcacgcc ctcgccagag acgtcgcggg
     2641 tgatcgacac gttctgcgtc ttacgggcta tcttatcgat ctcgtcgatg tagatgatgc
     2701 ccttctcggc gagcgatacg ttaccgccg
//
LOCUS       DAOPHV010000371         2718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_52450_length_2718_cov_4.188509, whole genome shotgun sequence.
ACCESSION   DAOPHV010000371 DAOPHV010000000
VERSION     DAOPHV010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52450_length_2718_cov_4.188509"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..804)
                     /locus_tag="PLQ29_09570"
     CDS             complement(49..804)
                     /locus_tag="PLQ29_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG86935.1"
                     /translation="MRFSVCTVTILSLVVALGSVVAVSAQEPSQAPAQPDLPAPAIAV
                     PAQAQPALPVKADALLLYRQGRDLETAGKTAEAAAKYRESVAVCDRELATDPTRMDAY
                     TVKCWSLFRLERYREVVDIGAAALKVRFDARVVEVMGEAYFHLGDDKSALKYLQRYLD
                     NVGEYGDRVSTAYFYMAESYYRMKRFDHADIAYSMAVYREPGLARWWFRYASAVESLG
                     EYTRAFDLYGKALKLSPSMAEAQSGQSRVKARM"
     gene            complement(909..2399)
                     /gene="gltA"
                     /locus_tag="PLQ29_09575"
     CDS             complement(909..2399)
                     /gene="gltA"
                     /locus_tag="PLQ29_09575"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPG86936.1"
                     /translation="MNLHNAGELGSKAKEVLAGLDGRELGNKDRMALPVQDMPCQDPL
                     DRAHNMEEVALGYTEAQARIEAARCLQCKNAPCVSGCPVSIDIPGFIAQVAKGDYHAA
                     ADVIRRTNLLPAICGRVCPQEKQCQAPCTLGKALKGVDKAVQIGRLERFVADRERAAG
                     GAPMPRVAAPTGKRVAVIGAGPAGLTVAADVRREGHEVVIFEAFQKPGGVMVYGIPEF
                     RLPKAIVREEAEKLERMGVSFELNYLVGRTRPLSALLEEDGFDAAFVGVGAGLPKFMG
                     IPGENLVGVFSANEYLTRANLMRAWDRGRAATPIYPSRRVAVLGGGNVAMDSARMALR
                     LGADEVHVLYRRTRDEMPARAEEVGHAIEEGIVFDFLASPTEVLGDDKGAVRGLRVMM
                     YELGEPDASGRRSPVAIKGSERDVPYDTVIVAVGNDSNPLLAKTTPGLAVDKKGHVLV
                     DENQKSSLDGVWAGGDIVLGAATVILAMGEGRRAAAAINEYLSRGE"
     gene            complement(2396..>2718)
                     /locus_tag="PLQ29_09580"
     CDS             complement(2396..>2718)
                     /locus_tag="PLQ29_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86937.1"
                     /translation="MMKFCAEATRPFGVRTLASLNTIMIDGTGMCGGCRVSVGGQTRF
                     VCVDGPEFDAHQVDWDGMLLRLRAYKSQEDAAHKALHESGQCRLDAMAEAARTQAHAE
                     GGSR"
BASE COUNT          396 a          967 c          906 g          449 t
ORIGIN      
        1 gatagacccg gacccggtca gcctgctcta gcggggtagc ggcccggcct acatcctggc
       61 cttgacgcgg ctctgtccgc tctgcgcctc ggccatgctc gggctaagct tgagggcctt
      121 gccgtagagg tcgaaggcgc gcgtgtactc gccgagcgac tcgaccgccg acgcgtagcg
      181 gaaccaccaa cgcgcgaggc cgggctcccg gtagacggcc atgctgtacg caatgtcggc
      241 gtggtcgaag cgtttcatcc gatagtagct ctcggccata tagaagtagg cggtggagac
      301 gcggtcgccg tactccccga cgttgtccag atagcgctgc aggtacttga gggcgctctt
      361 atcgtccccc aggtggaagt acgcctcgcc catcacctcg accacgcgcg cgtcgaagcg
      421 caccttgagg gcggcggcgc cgatgtcgac cacctcgcgg tagcgctcga gccggaacag
      481 gctccagcac ttgaccgtgt aggcgtccat cctcgtcggg tcggtggcca gctcccggtc
      541 gcagaccgct accgattccc tgtacttggc cgcggcctcg gccgtcttgc cggccgtctc
      601 caggtctcgc ccctgccggt acaggagcaa ggcgtcggcc ttgaccggca acgcgggttg
      661 cgcctgggcg ggaacggcta tcgccggagc cggcaagtcc ggctgggccg gcgcctggga
      721 cggttcctgg gccgatacgg ccacgacgga acccaaggcg acgacgaggc tgaggatagt
      781 gacggtacag acgctgaatc gcatgccaag actctccgta cgacagaaaa gggtcgggga
      841 cacgccgatc gacgcgccct cgacccccat tatggccgaa gccgggcttc ggatcaatga
      901 cccgcgccct attcgcctcg gctcaggtac tcgttaatcg ccgccgcggc tcgccggccc
      961 tcgcccatcg cgagtatcac ggtcgcggcg ccgagcacga tgtcgcctcc ggcccagacg
     1021 ccgtcgagcg agctcttctg gttctcgtcg accagcacgt ggcccttctt gtcgaccgcc
     1081 agacccggcg tcgtcttggc caggagcgga ttcgagtcgt tgcccacggc gacgatgacc
     1141 gtgtcgtagg gcacgtcccg ctccgagccc ttgatcgcga cggggctgcg cctaccggac
     1201 gcgtcgggct cgccgagctc gtacatcatc acgcggaggc cccggactgc gcccttatcg
     1261 tcgccgagca cctcggtggg gctggccagg aagtcgaaga cgatgccttc ctcgatcgcg
     1321 tgccccacct cctcggcgcg ggccggcatc tcgtcgcggg tgcgccggta caatacgtgc
     1381 acctcgtcgg cgccgagcct cagggccatg cgcgccgagt ccatcgcgac gttgccgccg
     1441 ccgagcaccg cgacccgcct ggacgggtag atcggcgtgg cggcgcgccc gcggtcccag
     1501 gcgcgcatca ggttggcgcg ggtcaggtac tcgttggcgc tgaacacgcc gacgaggttc
     1561 tcgccgggta tgcccatgaa cttcggcagg ccggcgccga cgccgacgaa ggccgcgtca
     1621 aagccgtcct cctcgagtag ggcggacagc ggccgcgtgc ggccgaccag gtagttcagc
     1681 tcgaagctca cgcccatccg ctcgagcttc tcggcctcct cgcggactat ggccttgggc
     1741 aggcggaact ccgggatacc gtagaccatc acgccgcccg gcttctggaa ggcctcgaag
     1801 atgaccacct cgtggccctc gcggcgcaca tcggccgcta cggtcaggcc ggcggggccg
     1861 gcgcctatca cggccacccg cttgccggtc ggggcggcca cgcggggcat cggcgcccct
     1921 cctgccgcgc gctcccggtc ggccacgaag cgctcgaggc gaccaatctg tacggccttg
     1981 tcgacgccct tgagcgcctt gccgagggtg cagggcgcct ggcactgctt ctcctgcggg
     2041 cagacgcggc cgcaaatggc cggtagcagg ttcgtcctgc gtataacatc ggccgcggcg
     2101 tggtagtcgc ccttggccac ctgcgctatg aagccgggaa tatctatgga taccgggcaa
     2161 ccggacacgc agggcgcgtt cttgcactgc aggcagcgag cggcctcgat cctggcctgg
     2221 gcctcggtgt agccgagcgc cacctcttcc atgttatggg cgcgatccag cgggtcctgg
     2281 cagggcatat cctgcacggg cagggccatc cggtccttgt tcccgagctc gcggccgtcg
     2341 aggccggcca ggacttcctt ggccttggag ccgagctccc ccgcgttatg caggttcatc
     2401 gcgagcctcc ctcggcgtgg gcctgggtcc tggccgcctc ggccatcgcg tcgagcctgc
     2461 actggccgct ctcgtgcagc gccttgtgcg ccgcgtcttc ctgcgactta taggcgcgca
     2521 atcggagtag catgccgtcc cagtccacct ggtgggcgtc gaactcgggc ccgtcgacgc
     2581 agacgaacct ggtctggccg ccgaccgaga cgcggcagcc gccgcacatg ccggtgccgt
     2641 ctatcatgat cgtgttgagc gacgcgagcg tcctcacgcc gaagggcctg gtcgcctcgg
     2701 cgcagaactt catcatca
//
LOCUS       DAOPHV010000372         2717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_52478_length_2717_cov_3.806912, whole genome shotgun sequence.
ACCESSION   DAOPHV010000372 DAOPHV010000000
VERSION     DAOPHV010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52478_length_2717_cov_3.806912"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="PLQ29_09585"
     CDS             <1..1418
                     /locus_tag="PLQ29_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HPG86938.1"
                     /translation="PRGRGLTDYLNDASPRTIEVIENKAAMAAFPEAAEAMRSQVARY
                     RKRDDMNAATIKRFGRLWVRNLAVNLRQAAASPGVSAIAGAFSGLPALVLAAGPSLDD
                     LADSLPDLAERCVVVCVDTALRSALRYGVTPDFVVVVDPQYWNARHLDRCRADGAILV
                     SEAAVWPSVLRGESWRPGGRTVLCSSIYPLGAYVESRLGQVQGRLGAGGSVSTTAWDF
                     ARTLGCSPIYMAGLDLSFPDGRTHARASLFEQRALASGDRLRPSSNAAFEAMRGGRPF
                     TATANDGSAVVSDERLSLYASWFSCNAAMHPEARTVNLSNRGMAIDGLASAGPEEPLA
                     LAPARDRIGSCLRAATAAMEAAAQADPDAVGLIVDGLVGELSRVAALAERAVGIARGA
                     IGRADLGSALAELSAIDDAVLGSEAKAVVGFIVDTAATSAKAVHGYGVGSLERTAELY
                     GAIAESARWHAERLRAAITEK"
     gene            complement(1597..2412)
                     /locus_tag="PLQ29_09590"
     CDS             complement(1597..2412)
                     /locus_tag="PLQ29_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPG86939.1"
                     /translation="MRKYIATLMTLLALFAVASVGAQAKIADGFYFAEEAAFPSSGWK
                     YQVVLQVKGGKIVSATWNGINALGLEDKKTIASSGRNGMVKASKLGKEWHEQAAVVEK
                     YLVDTQDLSFAKYTTPAGNTDAISGVSIHVKEFFDLAKAAVAAGPVAKGSYKKDGWFY
                     AKAAAFDKSGYMTTVLITVVNGRVVSANWNALNKAGGDTKFVRAAKGTYKMGAKQGEW
                     NVQSPRVEAELIRIQDPAKIAVKADGHTDAISGVSILVDEFVTLAAQALKAAK"
BASE COUNT          470 a          867 c          864 g          516 t
ORIGIN      
        1 gccctcgagg gcggggactg accgactacc ttaacgacgc atctccgcgg accatcgagg
       61 taatcgagaa caaggccgcc atggccgcgt tcccggaggc ggccgaggcc atgcgatccc
      121 aagtcgcgcg gtatcgaaag cgcgacgata tgaacgcggc gacgatcaag cgcttcggca
      181 ggttatgggt gagaaacctg gccgtcaacc tgcggcaggc cgcggcttcg cccggcgtat
      241 ccgctatcgc cggcgccttc tcgggtttac cggctctggt actggcggcc gggccgtcgt
      301 tggacgacct agccgattcg ctaccggacc tggccgagcg ttgcgttgtc gtctgcgtcg
      361 acacggccct gcgatcggcg ctacgctacg gcgtgacgcc ggacttcgtc gtcgtcgtcg
      421 acccgcaata ctggaacgcc cgccacctgg accggtgccg ggccgacggt gcgatactgg
      481 tgtccgaggc cgctgtctgg ccgtcggtcc tgcgcggcga gtcgtggcga cccggcgggc
      541 gaacggtcct ctgctcgtcg atctatcccc tcggagccta cgtcgagagc cgactcggcc
      601 aggtacaagg gcggctcggg gccggcggat cggtatcgac gacggcctgg gacttcgcgc
      661 ggacgctcgg ctgctcgccg atctacatgg cgggcctcga cttgtcgttc ccggacggca
      721 ggacccacgc gagggccagc ctattcgagc aacgggccct cgcgtcgggc gaccggctga
      781 ggccttcctc caacgccgct ttcgaggcga tgcgcggcgg caggccgttc accgccacgg
      841 ccaacgacgg ctccgcggtc gtcagcgacg agcgcctgtc gctctacgcc tcgtggttct
      901 cctgcaacgc ggccatgcac ccggaggccc gtaccgtgaa tctgtcgaac cgaggcatgg
      961 cgatagacgg actagcgtct gccggtcccg aggagccgct cgccttggcg cccgccaggg
     1021 ataggatagg atcatgcctg cgcgccgcca ccgccgcgat ggaagcggcg gcccaggccg
     1081 atccggacgc cgtaggcctc atcgtcgacg gcctcgtcgg cgagctatcg cgggtcgccg
     1141 ccctggccga gcgggccgtc ggcatagccc gtggagccat cggccgcgcc gatctcggct
     1201 cggccctggc cgagctatcc gccatagacg acgccgtgct cggtagcgaa gccaaggccg
     1261 tagtcggctt catcgtcgat acagccgcta cctccgcgaa ggccgtccat gggtacggcg
     1321 tcggtagcct cgaacgtaca gccgagctat acggcgcaat cgccgagagc gcccgttggc
     1381 acgctgaacg cctccgcgcg gcaattaccg aaaaatagcg cttatcatcc gtacctttac
     1441 gcccgatata ttgtataggc gctgaaagcc tgaggtagaa cggtatgcaa tgacgtccag
     1501 gcataccgtt ctttttttct atagcggggc gtacgcttcg tcgctataga aaaaaggccg
     1561 tccggatcgg acggccttct cgctagtcga aaacgcctat ttggcggcct tgagagcctg
     1621 agctgcgaga gtcacgaact cgtctacgag aatcgatacg ccggagatag cgtcggtgtg
     1681 gccgtcggcc ttgacggcga tcttggccgg atcctggatc ctgatcagct cggcctcgac
     1741 gcgcggggac tggacattcc actcgccctg cttggccccc atcttatagg tacccttggc
     1801 cgctcggacg aacttggtgt cgccgccggc cttgttgagg gcgttccagt tggcgctgac
     1861 gacgcggccg ttgacgaccg tgatcagcac ggtcgtcatg taaccgctct tgtcgaaggc
     1921 ggcggccttg gcgtagaacc agccgtcctt cttgtagctg cccttggcga cgggacccgc
     1981 ggccacggcg gccttggcca ggtcgaagaa ttccttcaca tgaatcgaaa cgccggagat
     2041 ggcgtcggtg ttgccggcgg gtgtggtgta cttggcgaag gacaggtcct gggtgtcgac
     2101 gagatacttc tcgacgacgg ccgcctgctc gtgccactcc ttgccgagct tggaagcctt
     2161 gaccatgccg ttgcggccgg acgaggctat cgtcttcttg tcctcgaggc cgagggcgtt
     2221 gataccgttc caagtagccg acacgatctt gccgcccttg acctggagca ctacctggta
     2281 cttccagccg ctcgacggga aggccgcttc ctcggcgaaa tagaaaccgt cagcgatctt
     2341 ggcctgggcc ccgaccgatg cgacggcgaa cagcgccagc aacgtcatca gcgtcgcgat
     2401 atacttcctc attcgtccct ccaatgggtc ttaagactat catccgtccg atggcggata
     2461 gtataatata gtaagattat tatagattaa atagtcaata gaaggaaaag gtaacggcat
     2521 ggaggcatgg ccggagttga ctagacgatc acgacgctgt tgtcggagtc cctgaacgga
     2581 ctctggacgt acttgtcggc atgccagtca ttatcccagc gttcgccgtc gagcaagtac
     2641 ctgaaatggt attcttttcc ggcctcgagc ttgatgccca gttcttcgga gatgacttcg
     2701 cccgaagtca ggatcgc
//
LOCUS       DAOPHV010000373         2708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_52729_length_2708_cov_3.363739, whole genome shotgun sequence.
ACCESSION   DAOPHV010000373 DAOPHV010000000
VERSION     DAOPHV010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52729_length_2708_cov_3.363739"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2468
                     /locus_tag="PLQ29_09595"
     CDS             <1..2468
                     /locus_tag="PLQ29_09595"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPG86940.1"
                     /translation="SGVVTLVSAGEATITATRAATSAYESVSASYALTVTALSPSTIA
                     FADGATVNRLLGSGPYVNAVSGEGSGSLSYRSSVPAVAAVDAASGQVDVVGLGTTTIS
                     VDKAATATHASASASYTLIVTALPLSTIVFADGGSVSRTLGTGPYTNAVSGEGDGAIS
                     FESSSPAVASVDAATGEVTMVSQGTAVITASKAATATHEAASASYTLTVLSPSTIVFA
                     DGASVTRTWSTTLYINAVSGDGSGALSYASGEPGVATVDASSGEVSLVAAGSTVISVS
                     KAATATHAAVESSYSLTVTKAASVISFADGTSVSRAYGSGSYINVVSGAGTGAKTYSS
                     SETAVATVNAAGSVLPLLVGSTEITVNKAATATHAAASASYALTVSPAASTITFLDGT
                     SVTKRLDESPYTNAVSGDGSGAVSYSSNNTGVATVASNGAVTLVSGGTAVITASKAAT
                     STHASASASYTLTVLYPSTIVFADGASVHRTLGSGTYTNAVSGDGSGAISYSSSNPAV
                     ATVNASSGTANVLALGSTVITATKAATATHAAVVASYTLTVEFLALVDVPAGTFQRDS
                     TPTNTAYVSAFRIASTELTRAQYLDVMGVDPQQGASYSTGVNDPVLNTNWYHAIAFCN
                     KLSLREDLEPVYTVYVGGSAVDFSSLAYASIPTAANADWNNAVADWDADGYRLPTSHE
                     WLWAAMGADTANPGALNTTGYAKAFAGSTGANVVNDYSWSAANSGSKTHPAGGKYPNE
                     IGLYDMSGNVSEWCWDWMHNPYPTGALINYKGGTGSIANRICAGSSRFTNENAGRFLS
                     NFSLDYNGPDSPSKGLRVVRNAE"
     gene            2524..2697
                     /locus_tag="PLQ29_09600"
     CDS             2524..2697
                     /locus_tag="PLQ29_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86941.1"
                     /translation="MKASVEIRDEVLEELYAYYLGPGEFYGPDELLDRLCQTVIALAE
                     LEQDASKKNSPRR"
BASE COUNT          503 a          961 c          843 g          401 t
ORIGIN      
        1 cgtcgggcgt cgttacgctc gtctccgccg gcgaagcgac gatcacggcg acgagggccg
       61 cgacgtcggc ctacgagtcg gtcagcgcgt cctatgcgct caccgtgacg gcgctatcgc
      121 cctcgaccat tgccttcgcc gacggcgcta cggtgaaccg gctcctcggt agcggcccct
      181 acgtgaacgc cgtctcggga gagggctcgg gcagcctgag ctaccggagc agcgtcccgg
      241 ccgtagccgc ggtagacgcg gcgtcgggcc aggtcgacgt ggtcggcctg gggacgacga
      301 ccataagcgt cgacaaggcc gcgacggcca cgcacgcctc ggcgagcgcc tcgtataccc
      361 ttatcgtcac ggccctgccg ctatccacca tcgtattcgc ggacggcggc tcggtgagcc
      421 gtacgctcgg gaccgggccg tacacgaacg cggtgtcggg cgagggcgac ggcgcaatct
      481 ccttcgagag ctcgtccccg gccgtcgcct cggtcgacgc ggcgacgggc gaggtgacca
      541 tggtctccca gggcacggcc gtaatcacgg cgtccaaggc cgccaccgcc acccacgagg
      601 cggccagcgc ctcgtatacc ctgaccgtac tctcgccttc gacgatagtc ttcgccgacg
      661 gcgctagcgt cacgaggacc tggtccacga ccctctacat caacgcggta agcggcgacg
      721 gctccggcgc gctgagctac gcgagcggcg aaccgggcgt cgcgacggtc gacgcctcga
      781 gcggcgaggt cagcctcgta gcggccggct ccacggtaat atcggtcagc aaggccgcca
      841 cggccaccca cgcggccgtc gagtcgtcct actcgctgac cgtgaccaag gccgcgtcgg
      901 tgataagctt cgccgacgga acgtccgtct cgagggccta cggtagcggt tcgtatatca
      961 acgtcgtcag cggcgcggga acgggagcca aaacctactc gagtagcgag acggccgtgg
     1021 cgacggtcaa cgccgccggc tcggtcctac ctctactggt tggcagcact gaaataacgg
     1081 ttaacaaggc cgccaccgcc acccacgcgg ccgctagcgc ctcctacgcg ctgacggtct
     1141 ctccggccgc ctcgaccatc accttcctcg acggaacctc ggtgaccaag cggctcgacg
     1201 agagccccta caccaacgcc gtctcgggcg acggctcggg cgccgtcagc tactcgagca
     1261 acaacacggg cgtagccacg gtagcctcca acggcgcggt caccctggtc agcggcggca
     1321 ccgcggtaat caccgccagc aaggccgcca cctccaccca cgcctcggcg agcgcatcgt
     1381 acacgctgac cgtgctctac ccgtcgacga tagtcttcgc cgacggcgcg tcggtccata
     1441 gaaccctcgg tagcggcacc tacaccaacg cggtcagcgg cgacggatcg ggcgcgatca
     1501 gctactcgag ttccaacccg gcggtcgcca ccgtcaacgc gagctcaggt acggctaacg
     1561 tcctagccct cggcagcacc gtgatcaccg cgaccaaggc ggccaccgcg acccacgcgg
     1621 cggtcgtcgc cagctatacc ctgaccgtgg agttcctcgc cctggtcgac gtcccggccg
     1681 gcaccttcca gagggacagc accccgacga acaccgccta cgtcagcgcc ttccgcatcg
     1741 cgagcacgga gctaacgagg gcccagtacc tggacgtgat gggggtagac ccgcagcagg
     1801 gagcgtcgta ttcaaccggc gtcaacgacc cggtgttgaa taccaactgg taccacgcga
     1861 tagccttctg caataagctc agcctgcgcg aggatctcga gccggtctat acggtctacg
     1921 tgggcgggtc ggccgtcgac ttctcgagcc tggcgtacgc ctccatcccg accgccgcca
     1981 acgccgactg gaataacgcc gtggccgatt gggacgccga cggctatagg ctccccacgt
     2041 cgcacgaatg gctctgggcg gctatggggg ccgatacggc caatcccggc gccttgaata
     2101 ccacgggcta cgccaaggcc ttcgccggca gtaccggcgc caacgtcgtc aacgactact
     2161 cgtggtcggc ggccaatagc ggaagtaaga cccatccggc gggcggaaaa tatcccaacg
     2221 agatcggcct ctatgacatg agcggcaacg tatcggaatg gtgctgggac tggatgcaca
     2281 atccctatcc tacgggcgcc ttgatcaact ataaaggcgg gaccggatcg atcgcgaacc
     2341 gcatctgcgc cggatcgtcg cgattcacca atgagaacgc tggtaggttc ttgagcaact
     2401 tcagcctgga ctacaacggc cccgactcgc cgagcaaggg cctacgcgtt gttcgcaacg
     2461 ccgagtagag tatatacggc tcccgcccag gcgggagccg tagcgaaacg gaggaatcgg
     2521 gccgtgaagg ctagcgtcga gatacgcgac gaggtactcg aggagctcta cgcctactac
     2581 cttggccccg gcgagttcta cggccccgac gagctcctcg accgcctctg ccaaaccgtc
     2641 atcgcccttg ccgagctcga gcaagacgcc agcaaaaaaa acagcccgag gcgctagcct
     2701 cgggctgt
//
LOCUS       DAOPHV010000374         2701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_52906_length_2701_cov_4.768707, whole genome shotgun sequence.
ACCESSION   DAOPHV010000374 DAOPHV010000000
VERSION     DAOPHV010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52906_length_2701_cov_4.768707"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="PLQ29_09605"
     CDS             <1..991
                     /locus_tag="PLQ29_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPG86942.1"
                     /translation="LFGLNVDDDPRSVASLAGAVRDACSVDTARIRSLPPFVAIDHEG
                     GGVYRFKGGLTRLPAAATMGLAGAAAASAAGSAAGTELRALGVTMNAAPVVEALNEAN
                     RGFLAGRAWSDDPAEAAKLSASFIVSCQRGGAAAVAKHFPGNAEVDPHRGLPVLTASR
                     AVVEGAYLAPFRAAVNAGVAAIMLSHAIVPAIDPDRPVSVSPKAVAILKGELGFSGIV
                     LTDDLQMAALDGYGDPGLSAVAALAAGADMVMLTGGRTALGARDAILAALADGRLTRA
                     RLRDAAVRVIAQKLRFGLDAETDGERDARLRSLERTVQANTAALKAAMRESAR"
     gene            1060..2313
                     /locus_tag="PLQ29_09610"
     CDS             1060..2313
                     /locus_tag="PLQ29_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HPG86943.1"
                     /translation="MRTNALGCISIVLLLSLAEASAQPLTSALAVTSSDLRIEQRDDA
                     GYHLFVRAKPGIGSVLLVETTKDPSGAADNYAYRAEAWNPINGDERRMLDGAFIDPAR
                     GLWSLIDSTPEPDASFGSAYHIFIPWVVAYGYPWSRNGREFIADGTFVNIRAFAKPYA
                     DYSGGFLDNPYRISVTQKPFPRPEPRALPAIEPPAAVVEPPKPEPPAAVVEPPKPEPP
                     KPEPKPVDTSIYMPETLRSFESIAASGKGTVSYSLGEADIVPTLERILDSVEGESLDL
                     VICLDTTESMADDIDAVKTSLPAMVAEKTKRFSAFRLGLVLYKDYFEDYVVKRLEFTQ
                     DVGAFTDAVTRIRVAGGRDIPEAVYEALYEALIGYPWSADAMLIVLIGDAPAHPLPRG
                     RVGKAMVDAKSAELGVAISVIVLPH"
     gene            2410..>2701
                     /locus_tag="PLQ29_09615"
     CDS             2410..>2701
                     /locus_tag="PLQ29_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86944.1"
                     /translation="MRSKRARTKETEPLERVASLGVLALSVAVFVFGFAFVSSGFPVS
                     YAEPAESVGAADLADATGSAGIADAESPDGLDAIVDEVPPPDDGLFYMAYRVV"
BASE COUNT          426 a          955 c          889 g          431 t
ORIGIN      
        1 actcttcggg ctcaacgtcg acgacgatcc ccggtcggta gcctcgctcg cgggggcggt
       61 ccgcgacgcc tgctccgtcg acacggcccg gatccgctcc ctgccgccct tcgtcgccat
      121 cgaccacgag ggcggcggcg tctatcgctt caagggcggc ctgacacggc ttccggccgc
      181 cgcgacgatg ggtctggccg gcgcggcggc cgcttcggcc gccgggagcg ccgcgggaac
      241 ggagctccga gccctcggcg tcacgatgaa cgcggctccg gtcgtcgagg cgctcaacga
      301 ggccaatcgg ggcttcctcg ccggcagggc ctggtcggac gacccggccg aggcggccaa
      361 gttgtcggcc tccttcatcg tgtcctgcca gcgtggcggt gcggccgcgg tggccaagca
      421 ctttcccggc aacgccgagg tcgacccgca tcgcggcctg ccggtgctca cggcgtcccg
      481 cgccgtggtc gagggcgcct acctggcgcc gttcagggcg gccgtgaacg ccggcgtagc
      541 ggctatcatg ctgtcgcacg cgatcgtgcc ggccatcgac cccgaccgcc cggtcagcgt
      601 gtcgcccaaa gccgtggcga tactcaaggg agagctgggc ttctccggca tcgtcctaac
      661 cgacgatctc cagatggccg cgctcgacgg ctacggcgac ccgggcctga gcgcagtggc
      721 ggcgctcgcc gcgggggcgg acatggtcat gctgaccggg gggcgcacgg cgctcggcgc
      781 cagggacgcg atactggccg cgctggcgga cggcaggctg acccgggcac ggctccggga
      841 cgccgcggtc agggtgatag cccagaagct acgcttcggg ctggacgccg agaccgacgg
      901 cgagcgggac gcccgtctaa gatcgctcga gcgcacggta caggccaaca cggccgccct
      961 gaaagccgcc atgcgcgaat cggcgcgcta agcgcccggg gcggctagcc acggcgatcc
     1021 gcgcttacgt aggacggcct acgcgccgat tattacaagg tgagaacgaa cgctctaggc
     1081 tgtatctcga tcgtccttct cctgtcgctc gccgaggcct cggcgcagcc cttgacctcc
     1141 gctttggcgg taacctcgtc cgacctgcgc atcgagcagc gcgacgacgc cggctaccac
     1201 ctattcgtgc gggccaagcc cggcatcggc agcgtgctac tcgtcgagac gaccaaggac
     1261 ccgagcggtg cggccgacaa ctacgcgtat cgcgccgagg cttggaaccc tataaacggg
     1321 gatgagcgac gcatgctcga cggagccttc atcgatcccg cccgcggcct ctggtcgctc
     1381 atcgactcga cgcctgaacc ggacgcctcg ttcggctccg cctaccatat cttcatcccc
     1441 tgggtggtag cctacggcta tccctggtcg cgcaatggcc gcgagttcat cgccgacggc
     1501 accttcgtca atatcagggc cttcgccaag ccgtacgccg actactcggg cggttttctc
     1561 gacaacccgt accggatatc ggtcacgcag aaaccgttcc ctcggccgga acctcgcgct
     1621 ctgccggcca tcgagccgcc ggccgccgtc gtcgaaccgc cgaagcccga gccgccggcc
     1681 gccgtcgtcg agccgccgaa gcccgaaccg ccgaagcccg aacccaagcc ggtcgatacg
     1741 tccatctata tgccggagac gctgcgctcc ttcgagtcca tcgccgcgtc cggtaagggt
     1801 acggtatcgt attcgctcgg cgaggccgat atcgtgccga ccctcgagcg catcctcgac
     1861 tcggtcgagg gcgagtcgct cgacctggtc atctgcctgg ataccaccga gtcgatggcc
     1921 gacgatatcg acgcggtcaa gacgagcctc ccggccatgg tcgccgagaa gacgaagcgc
     1981 ttctcggcgt tcaggctcgg cctcgtgctc tacaaggact atttcgagga ctacgtcgtc
     2041 aagcgcctgg agttcacgca ggacgtcggc gcctttaccg acgcggtgac caggatacga
     2101 gtcgccggcg gtcgcgacat accggaagcc gtctacgagg cgctctacga ggccctgatc
     2161 ggctacccct ggtcagccga cgctatgctg atcgtgctga tcggcgacgc gcctgcccat
     2221 cccttgccta ggggcagggt aggcaaggcc atggtcgacg ccaagtccgc cgaactcggc
     2281 gtggcgatca gcgtcatcgt cctgccgcac tgaggcccgg cggcagctgt tttttgttta
     2341 cacgacggcc ggaggcgtat tagaatcgcg taccgaccag tgaacaccat cgaacggagc
     2401 cgagcgggga tgagaagcaa gcgagcgaga acgaaggaga cagaaccgct cgagcgggta
     2461 gcgagcctcg gggtactcgc gctctccgtg gccgtcttcg tcttcggctt cgccttcgta
     2521 tcgtcgggct ttcccgtctc ctacgcggaa ccggccgagt cggtcggagc cgccgacctg
     2581 gccgacgcca cgggctccgc cggtatcgcc gacgctgagt cgcccgatgg gctcgacgcc
     2641 atcgtcgacg aagtcccgcc ccccgatgac gggctgttct atatggcgta tagggtggtc
     2701 a
//
LOCUS       DAOPHV010000375         2675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_53588_length_2675_cov_3.838931, whole genome shotgun sequence.
ACCESSION   DAOPHV010000375 DAOPHV010000000
VERSION     DAOPHV010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53588_length_2675_cov_3.838931"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PLQ29_09620"
     CDS             <1..941
                     /locus_tag="PLQ29_09620"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPG86945.1"
                     /translation="VADDEGRYLQAAIRYIGAAGTEKQAAALRAAYEADGASQATKEA
                     VVQALGALRSVESFELLSSVASSEESSKAIRMYACSALGELGDDRAVPVLVGASVSSD
                     PNVRAAAVEALGTYSGADALAAVRQALRDAHALPRIAAAKASGESRDAEALPYLEYKA
                     SYDPEKAVREASIAALASIGGQRADDYLSAFLEEPKNGTQYRSVALKAILADGGGEAR
                     KKALAVFAAAQSEKDRAPFTAFAKAAMSVDDEAAAPFAELMLGDKDFSMRLGALAWIE
                     RNRYAALADAVRSVSETDASDAVKKRAAKALERLSP"
     gene            complement(958..>1766)
                     /locus_tag="PLQ29_09625"
     CDS             complement(958..>1766)
                     /locus_tag="PLQ29_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86946.1"
                     /translation="HASPYAADVGKPAAHASPYAADVGKPAAHASPYATDVGKPAAHA
                     SPYATDVGKPAAHASPYAAGVTAPEAAHIRDKAALLGAWGGTAASRRALPLASDASRE
                     ATAVTAPRGPMSYEPNVVRAGSARLTLRVLGQNPNIGKRNIRAMHSGGRASVGGGSSD
                     FLVFLLPVPRRIAWLYYDGSDATLVPAKPEFFPDYDAPIEACLGRDIRMVTARGKELT
                     LRFERYVSPVERINRLLHCIESPGLSAKPGDDGRSEADAVVALAEPESGV"
     assembly_gap    1767..1866
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1867..>2675)
                     /locus_tag="PLQ29_09630"
     CDS             complement(<1867..>2675)
                     /locus_tag="PLQ29_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86947.1"
                     /translation="SRYGALALGGSLGASAEASAQGQGSVAGGTGTAGYGEAGSNAPG
                     AGDYLADLAAAAGTAVSSFDPANASATVEDTIDLPRISFPAGLGATDYALSIPVEIGN
                     GSSRELRVELTRLLLDDGTDILREKAFATVPAGGSARVVLKALLPESMAEGRLELSLE
                     PRFADGLRVSPAKSVVAMELRRSPLAAFFRNSAMVVLFFVLLAVACAAILLVFLYSRR
                     IHRKAEEPVVDALIDASAQSRSAHVSPYAADVGKPAAHASPYAAANAGTNA"
BASE COUNT          414 a          844 c          934 g          383 t
ORIGIN      
        1 tagtcgccga cgacgagggg cgctacctcc aggcggcgat caggtatata ggggccgccg
       61 gcactgaaaa gcaggccgcg gccctgcgag ccgcctatga ggcagacggc gcgagccagg
      121 cgacgaagga agcggtcgta caggcgctcg gcgcgctacg gtcggtcgag tcgttcgagc
      181 tgctctcgtc cgtcgcgtcg tcggaggagt cgtcgaaggc catcaggatg tacgcctgct
      241 ccgcccttgg cgagctcggt gacgataggg ccgtgccggt actcgtcggc gcgtccgttt
      301 cgagcgatcc gaacgtccgc gcggccgcgg tcgaggcgct cggtacgtat tcgggcgccg
      361 atgcgctggc ggccgtcagg caggccttgc gcgacgccca cgccttgcca cgcatcgccg
      421 cagcgaaggc ctccggtgaa tcccgcgacg ccgaggccct gccatacctt gagtacaagg
      481 cttcctacga ccctgaaaag gcggtgcgcg aggcgtcgat cgccgctctc gcgagtatag
      541 gcggccagcg tgccgacgac tacctctcag cgttcctgga ggagcctaag aacgggacgc
      601 aatatcgatc cgtcgcgctc aaggccatct tggccgacgg cggcggcgaa gcgaggaaga
      661 aggcgctggc cgtgttcgcg gcggcccagt ccgagaagga tagggcgccg ttcacagcgt
      721 tcgccaaggc cgccatgtcg gtggacgacg aggcggcggc gcccttcgcc gagctgatgc
      781 tcggcgacaa ggatttctcg atgcgcctcg gggcgttggc gtggatcgag cggaaccgct
      841 acgccgcgct cgccgatgcg gtacgatccg tctcggagac ggacgccagc gacgcggtca
      901 agaagcgggc ggccaaggcg ctcgagcgcc tctcgccata gccgatcata gagatcccta
      961 cactccggac tcgggctcgg ccagcgccac gacggcgtcg gcctctgaac ggccatcgtc
     1021 gcccggcttg gctgagaggc ccggcgactc gatgcaatgg agcagcctgt tgatgcgctc
     1081 gaccggcgac acgtagcgct cgaacctgag cgtcagttcc ttgccacgcg cggtgaccat
     1141 gcgtatatcc ctgccgaggc aggcctcgat cggcgcgtca tagtccggga agaactcggg
     1201 cttggcgggt acgagggtgg cgtcgctacc gtcgtagtac agccaggcga tacgcctggg
     1261 caccggcaag agaaacacca ggaagtcgct cgagcctccg ccgaccgacg ctcgaccgcc
     1321 cgagtgcatc gcgcggatgt tgcgcttgcc gatgttcggg ttctggccga ggactctcag
     1381 ggtcaggcgg gccgagcccg ctcggaccac gttcggctcg tagctcatcg ggccgcgcgg
     1441 agccgttaca gccgtcgcct ctctggacgc gtcggaggcc aacggcaagg ccctgcgcga
     1501 ggcggcggta ccgccccagg cgccgagtag cgcggccttg tcgcgaatat gcgcggcctc
     1561 gggcgcggta accccggcgg cgtagggcga cgcgtgagcc gcgggctttc ccacgtcggt
     1621 ggcgtagggc gacgcgtgag ccgcgggctt tcccacgtcg gtggcgtacg gcgacgcgtg
     1681 agccgcgggc tttcccacgt cggcggcgta gggcgacgcg tgagccgcgg gctttcccac
     1741 gtcggcggcg tagggcgacg cgtgagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnccgc attcgttccg gcgttggcgg cggcgtaggg cgacgcgtga gccgcgggct
     1921 ttcccacgtc ggcggcgtac ggcgagacat gcgcggatcg gctctgagcc gaggcgtcga
     1981 tcagggcgtc gacgaccggt tcctcggcct tcctatggat ccgtctggag tacaagaaca
     2041 cgaggagaat ggcggcgcag gcgacggcca gtagcacgaa gaacagcacg accattgccg
     2101 agttcctgaa gaacgcggcg agcggcgaac ggcgtagctc catggcgacg accgacttgg
     2161 cggggctaac gcgaagcccg tcggcgaaac ggggttcgag gctcagctcg aggcgaccct
     2221 cggccatgga ttccgggagg agcgccttga gaaccacgcg agccgagcct ccggcgggca
     2281 cagtcgcgaa ggccttttca cgtaggatgt ccgtaccgtc gtcgaggagc aatcgggtca
     2341 gctcgacccg caactcccgg gaggagccgt tccctatctc gacaggtatg gataacgcgt
     2401 agtccgttgc accgagcccg gcggggaacg atatccgcgg aaggtctatc gtatcctcga
     2461 ccgtggcgga cgcgttggcc ggatcgaagg aactgacggc cgtacccgcg gcggcggcca
     2521 ggtcggctag gtagtctccc gcacccgggg cgttcgaacc ggcctccccg tagcctgcgg
     2581 taccggtgcc tcccgctacg gagccctggc cctgggcgga agcctcggcc gaggcgccga
     2641 ggcttccgcc cagggccagg gctccgtagc gggag
//
LOCUS       DAOPHV010000376         2668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_53784_length_2668_cov_3.885955, whole genome shotgun sequence.
ACCESSION   DAOPHV010000376 DAOPHV010000000
VERSION     DAOPHV010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53784_length_2668_cov_3.885955"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..622
                     /locus_tag="PLQ29_09635"
     CDS             83..622
                     /locus_tag="PLQ29_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPG86948.1"
                     /translation="MHFAVCDFLLDLVQNSVEAGAANVGIDVVESGGSIEATVEDDGK
                     GMDERELERAKDPFYTDGTKHARRKVGLGLPFLIQATEQAGGDFRISSRKGSGTRVSF
                     GFPVDGVDTPPLGDLPGLFLSAMCFDGDYELVIRRRAPARGVDYEIRRSEILEAAGPL
                     DDASALVAVREFLASHEAS"
     gene            640..1035
                     /locus_tag="PLQ29_09640"
     CDS             640..1035
                     /locus_tag="PLQ29_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPG86949.1"
                     /translation="MAKMTLEQLRKLREEKKGDMQKRDVEGKSIQIIVGMGTCGIAAG
                     AKQSFDEIVKAIDENKLSGDVIIRQTGCMGLCHVEPTVEVVMPGMPATIYGRVDAATA
                     RDIVVKHVVGKTLVENHIYDRPAVDIVKK"
     gene            1052..>2668
                     /locus_tag="PLQ29_09645"
     CDS             1052..>2668
                     /locus_tag="PLQ29_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPG86950.1"
                     /translation="MAYKNFILVCGGTGCESSRADDIYKELIASAEALGVKDDVQIVK
                     TGCFGFCEKGPIVKVLPSESFYVEVKPEDAREIIAEQVVKGREVARLLYKDDRQKDRI
                     KVEDIQFYQKQFRIVLRNCGVINPENIDEYIARDGYAALEKVLSSMKPDDVIEQLKIS
                     GLRGRGGAGFPTWRKWMFSKGVQADQKYVVCNADEGDPGAYMDRSTIEGDPHSVIEAM
                     TICGYTVGSTKGFVYIRAEYPLAIERLKIALKQAREYGLLGDNILGTGFSFDIEIRLG
                     AGAFVCGEETALLQSLEGNRGMPIPRPPFPAVKGLWGKPTIINNVETWANIPVIITKG
                     AEWFAKIGTETSKGTKVFALTGKVNNSGLVEVPMGTTLREIIFDIGGGIKGGKKFKAA
                     QTGGPSGGIITAEFLDTPIDYESLTSLGSMMGSGGLIVMDEDDCMVDVSRFYMEFCVD
                     ESCGKCSPCRIGTNQMHALLVKITKGQGTLDDLDKLEKIGLAMKKAALCGLGQTAPNP
                     TLASIKRFRDEYVEHIVDRKCRAGKCKDLVV"
BASE COUNT          544 a          858 c          857 g          409 t
ORIGIN      
        1 cggcgacgaa gtctacggca agctgaccaa ggaccaggtc cccgagatca tcgccaagta
       61 caagagcctc ggctaggcgc ccgtgcactt cgccgtctgc gactttctgc tcgacctcgt
      121 gcagaattct gtggaggccg gagcggcgaa cgtcggtatc gacgtcgtcg aatcgggcgg
      181 atccatcgag gcgacggtcg aggacgacgg caagggcatg gacgaacgcg agctcgagcg
      241 cgccaaggac ccgttctata ccgacgggac caagcacgcc aggaggaagg tcggcctagg
      301 gctaccgttc ctcatccagg cgacggagca ggccgggggc gacttccgga tctcctcccg
      361 caagggttcg ggtacgcgcg tctcgttcgg cttcccggta gacggcgtcg atacgccgcc
      421 gctcggggat ctacccggcc ttttcctatc ggcgatgtgc ttcgacggcg actacgagtt
      481 ggtgatacgg cgacgggccc cggcccgcgg cgtcgactac gagatcaggc ggtccgagat
      541 actcgaggcc gcaggacccc tggacgacgc gagcgcgttg gtagcggtcc gagaattcct
      601 ggcctctcac gaggcctcgt gacgaaagga agcattcata tggcaaagat gacccttgaa
      661 cagctccgga agctccgcga ggagaagaag ggcgatatgc agaaacgcga cgtcgaagga
      721 aagagcatac agatcatcgt cggcatgggc acctgcggta tcgccgccgg cgcgaagcag
      781 agcttcgacg agatcgtcaa ggcgatcgac gagaacaagc tgtccggcga cgtgatcatc
      841 aggcagaccg gctgcatggg cctctgccac gtcgagccga cggtcgaggt cgtgatgccc
      901 ggcatgccgg ccacgatcta cggcagggtc gacgcggcca ccgcccgcga tatcgtcgtc
      961 aagcacgtcg tcggcaagac gctcgtcgag aaccacatct acgaccggcc cgcggtcgat
     1021 atcgtcaaga aatagggcgg aggccgaaat catggcgtac aagaacttca tactggtctg
     1081 cggcggcacc ggatgcgaat ccagccgcgc cgacgacatc tacaaggaac tcatcgccag
     1141 cgccgaggcg ctgggcgtca aggacgacgt ccagatcgtc aagaccggct gcttcggttt
     1201 ctgcgagaag ggcccgatcg tcaaggtcct gccctccgag tcgttctacg tcgaggtcaa
     1261 gcccgaggac gcgcgcgaga tcatcgccga gcaggtggtc aagggccgcg aggtcgcgcg
     1321 cctgctctac aaggacgaca ggcagaagga ccgcatcaag gtcgaggaca tccagttcta
     1381 ccagaagcag ttccgcatcg tcctgcgcaa ctgcggcgtg atcaatccgg agaacatcga
     1441 cgagtatatc gcccgcgacg gctacgccgc tctcgagaag gttctgtcgt cgatgaagcc
     1501 ggacgacgtg atcgagcagc ttaagatttc cggcctccgc gggcgcggcg gcgccggctt
     1561 cccgacctgg cgcaagtgga tgttctccaa gggcgtccag gccgaccaga agtacgtggt
     1621 ctgcaacgcc gacgaaggcg acccgggcgc ctacatggac cgctcgacga tcgagggcga
     1681 cccccactcg gtcatcgagg cgatgacgat ctgcggctac accgtcggct cgaccaaggg
     1741 cttcgtctac atccgcgccg agtacccgct cgccatcgag cggctcaaga tagcgctgaa
     1801 gcaggcccgc gagtacggcc tcctcgggga caacatcctc ggcaccggct tctcgttcga
     1861 tatcgagatc cgcctcggcg cgggcgcctt cgtctgcggc gaggagacgg ccctgctgca
     1921 gtcgctcgag ggtaaccgcg gcatgccgat cccgcgcccg ccgttcccgg cggtcaaggg
     1981 cctctggggc aagccgacga tcatcaacaa cgtcgagacc tgggccaaca tcccggtcat
     2041 catcaccaag ggcgccgaat ggttcgccaa gatcggcacc gagaccagca agggcaccaa
     2101 ggtgttcgcg ctcacgggca aggtgaacaa ctccggcctc gtcgaggtcc cgatgggcac
     2161 gacgctccgc gagatcatct tcgacatcgg cggcggcatc aagggcggca agaagttcaa
     2221 ggccgcgcag accggcggac cgtccggcgg catcatcacc gctgaattcc tcgacacccc
     2281 gatcgactac gagagcctga cctccctcgg ctcgatgatg ggctccggcg gactcatcgt
     2341 catggacgag gacgactgca tggtcgacgt gtccaggttc tacatggagt tctgcgtcga
     2401 cgagtcctgc ggtaagtgct cgccctgccg catcggcacg aaccagatgc acgccctact
     2461 cgtcaagatc accaagggcc agggcacgct cgatgacctc gacaagctcg agaagatcgg
     2521 cctggcgatg aagaaagccg ccctctgcgg gctcggccag accgcgccga acccgacgct
     2581 cgcgagcatc aagcgcttca gggacgagta tgtcgagcat atcgtcgacc ggaagtgccg
     2641 cgccggcaag tgcaaggatc tcgtcgtc
//
LOCUS       DAOPHV010000377         2652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_54206_length_2652_cov_3.562957, whole genome shotgun sequence.
ACCESSION   DAOPHV010000377 DAOPHV010000000
VERSION     DAOPHV010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54206_length_2652_cov_3.562957"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLQ29_09650"
     CDS             complement(<1..210)
                     /locus_tag="PLQ29_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase accessory protein HypB"
                     /protein_id="HPG86951.1"
                     /translation="MDAFKVLEIKKSVYEGNDKRAGALRSGLKKDGVFLLNLMSSPGS
                     GKTTTVMRTIEALRGDMRIGVLEADI"
     gene            complement(389..1648)
                     /locus_tag="PLQ29_09655"
     CDS             complement(389..1648)
                     /locus_tag="PLQ29_09655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG86952.1"
                     /translation="MDSGKTRVYGYRWVVLGAFMMVNLAIQLLWISYAPVTGQAAALY
                     GVSDLKIGMFSMLFMLAFIPLSIPVSWAIDTLGFRVTVGVGSALMGAFGVMRGLAGDG
                     YGLAFAATVGLAAAQPFLLNAWTKVPALWFPPGERATAVGLVTLANLVGTALGMVLTP
                     ALAESLPIPTIQLYYGAFSAACAAIFIVVAREKPATPPSDDAGQARSLMLEGLRHAFA
                     VPSFRRYLVLSFVGLGIFNGVTTWIEAIVRPRGFGPSEAGTLGAVMLVAGVVGAVLLP
                     ALSDKAGRRKPFLGLGLALAIPGLAGIALAQSFALLCASAAALGFFLTSVMPTGMQYA
                     SEVTRPTPEGTSNGLIQLFGQASVAFVYLMEAMRGPDGSFTLALIVACGLLAVSALVT
                     ISLTESSPLASASMAADPSGETGSSRG"
     gene            1704..2375
                     /locus_tag="PLQ29_09660"
     CDS             1704..2375
                     /locus_tag="PLQ29_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPG86953.1"
                     /translation="MAGTNAPAPRWTPVFERISDEKRRRVLESAKAAFARDGFAGTNV
                     NHVAAAADISVGALYKYFRTKDDLFLAIIEESHALIETTLAGIFAAEPSFFGRVEAIL
                     RAAISSSRDDPELVRMYVACTTEELSPLAERLSGSIETISATQYSRMVAEAAAAGEIA
                     SDVDPALTAFILDDIFLMIQFSFASTYYRDRLRLFAGGAADDDEAIVAAALAFIRRAL
                     ERRQS"
     gene            complement(2431..>2652)
                     /locus_tag="PLQ29_09665"
     CDS             complement(2431..>2652)
                     /locus_tag="PLQ29_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HPG86954.1"
                     /translation="NSGALGAAMLCAKGLGMMASLADARTMVRPKEVFEPNASLAGLY
                     AERFSVFKRFYYRNKPLFAALNGQRARPA"
BASE COUNT          475 a          891 c          853 g          433 t
ORIGIN      
        1 tatgtcggcc tcgaggacgc ctatgcgcat gtcgccgcga agcgcctcta tcgtgcgcat
       61 caccgtcgtc gtcttgcccg agccgggcga cgacatcagg ttgaggagga acacgccatc
      121 cttcttgagc ccggagcgga gcgccccggc ccgtttgtcg ttgccttcat agacgctctt
      181 cttgatttct aggactttga acgcgtccat gcgcttccta cctcccgttc tcgtaatcgg
      241 atatattatt accgacacct atgtcgatat tcgttcaaat aaaaagtcgt cctaagctct
      301 cgagccgaat accgaatgat atcgggactc ggctcgggag agcctgacgg cctctacggg
      361 ctattattag tccattttta tcgattcgtc aacctcgaga tgagcccgtc tcgccgcttg
      421 ggtctgcggc catcgaggcc gaggccaggg gcgacgactc cgtgaggctg atcgtcacga
      481 gagcgcttac ggcgagcagg ccgcaggcga cgatgagcgc cagggtaaag gagccgtcgg
      541 ggccgcgcat cgcctccatg aggtagacga acgcgaccga ggcctgcccg aacagctgga
      601 tgagcccgtt cgaggtgccc tcgggggtgg ggcgggtcac ctcggacgcg tactgcatgc
      661 cggtcggcat cacgctggtc aggaagaagc cgagcgccgc cgccgaggcg cataggaggg
      721 cgaagctctg ggcgagcgcg atgccggcca ggccagggat cgccaaggcc agcccgaggc
      781 cgagaaaggg cttgcgcctg cctgccttgt cggacagcgc cggcagcagc accgcgccca
      841 cgacgcctgc gacgagcatc accgcgccca gggtgcccgc ctcggacgga ccgaagccgc
      901 gcgggcgcac gatagcctct atccaggtgg taacgccgtt gaagatcccg aggccgacga
      961 aggacaagac gaggtaccgc ctgaagctcg gcaccgcaaa ggcgtgcctg agtccctcga
     1021 gcatcagcga gcgcgcctgg ccggcgtcgt cgctcggcgg cgtggcgggc ttctccctgg
     1081 cgaccactat gaagatagcc gcgcaggcgg cggagaaggc gccgtagtac agctgtatcg
     1141 tcggtatggg caggctctcc gccagggccg gcgtgagtac catgccaagg gccgtgccga
     1201 ccagattggc cagcgtcacg aggccgacgg ccgtcgcccg ctcgccaggc gggaaccaga
     1261 gcgctggcac cttggtccag gcgttgagca ggaagggctg agccgcggcc agcccgacgg
     1321 tggccgcgaa ggccaggccg tagccgtctc cggccaggcc gcgcatcacg ccgaacgcgc
     1381 ccatcagcgc ggagccgacg ccgacggtga ctctgaagcc gagcgtgtct atggcccacg
     1441 acaccggtat ggacaggggg atgaaggcta gcatgaagag catcgaaaac atgccaatct
     1501 tcaggtcgct caccccgtac agcgcggcgg cctggccggt gaccggggcg tacgatatcc
     1561 ataggagctg gatggccagg ttgaccatca tgaaggcgcc gagtacgacc caccggtagc
     1621 cgtacaccct agtctttccg ctatccatcg tttcctccat tacaatgtcg acgtggcggg
     1681 tcgtcccgcg ctatagtgca gccatggcag gaacaaacgc tccggcgccc cgatggacgc
     1741 cggtcttcga acggatctcc gacgagaagc gtcgccgcgt gctcgagagc gccaaggccg
     1801 cattcgcccg cgacgggttc gccggtacca acgtcaacca cgtcgcagcg gccgccgaca
     1861 tctcggtcgg ggcgctgtac aagtacttca ggaccaagga cgacctcttc ctcgccatta
     1921 tcgaagaatc gcacgccctg atcgaaacca ccttagccgg catattcgcc gccgagccta
     1981 gtttcttcgg cagggttgag gccatcctgc gcgccgcgat cagctcgtct cgcgatgacc
     2041 ccgagctggt gcgcatgtac gtggcctgca ccaccgagga gttgtcgccc ctcgcggagc
     2101 ggctgtccgg cagcatcgag acgatctccg ccacccagta cagccgcatg gtggccgagg
     2161 ccgccgcggc cggggagata gcctcagacg tcgacccggc gctcaccgcc ttcatcctcg
     2221 acgacatctt tctcatgatc cagttctcgt ttgcctccac ctactatcgc gaccgcctgc
     2281 gcctcttcgc gggcggggcc gcggacgacg acgaggccat cgtcgccgcg gcgctcgcct
     2341 tcatccggcg cgcactcgag aggcgccagt cgtaacggcg cgtcgcctac gtcgtaacgg
     2401 cgcgtcgcct acgtcgtaac ggcgcgtcgc ctacgccgga cgggcccgct ggccgttgag
     2461 cgccgcgaag agcggcttgt tcctatagta aaaacgcttg aacaccgaga atcgctccgc
     2521 gtacagcccc gcgagcgagg cgttcggctc gaagacctcc ttgggcctga ccatggttct
     2581 cgcgtcggcg agcgaggcca tcatgccgag ccccttggcg cagagcatgg ccgcgcccag
     2641 ggcgcctgag tt
//
LOCUS       DAOPHV010000378         2648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_54314_length_2648_cov_4.500964, whole genome shotgun sequence.
ACCESSION   DAOPHV010000378 DAOPHV010000000
VERSION     DAOPHV010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54314_length_2648_cov_4.500964"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PLQ29_09670"
     CDS             <1..948
                     /locus_tag="PLQ29_09670"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPG86955.1"
                     /translation="PCEVYEMIGSSPEATLSPAERDRYIVERLRAAGEPLSGESLAAE
                     LGVSRVALWKRVASLRAWGYGIEASRRGYELVRDDGLAGWELEAPGPVVLFDTVESTM
                     DEARALAEAGAPSGATVLALGQRSGRGRAGGAWDSPAGGLYLSTALRSSLPPSHAGAL
                     FLEAAAATLHALEAAGAPGLSYSWPSELRRGGEKVGGILVEAYGDIASADYYVVGVGL
                     SVAPGLLAEGSTARRTSVAAALVRELVAWCAAPSLELGRWDSLAPPAGSHMSVELWNG
                     ERRAFEPVGFDTRGDLTGADGTRLSIGEYRSHSIEGEYQ"
     gene            945..1571
                     /locus_tag="PLQ29_09675"
     CDS             945..1571
                     /locus_tag="PLQ29_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HPG86956.1"
                     /translation="MKRNGFKLVLSSLFAAMIAMGAVFSLPLPPPLPPVTLAVFFSLL
                     SGLLLGPRWALATTGLYLGLGAVGLPVFANGAGGLGQLAGPTGGFLVGYVAAAVVAGA
                     VSDRRRWGFGRAVAGALAGVAALYIVGLPRFRAVLDAGPDRSVTMGAAFLMMAPYLVG
                     DMAKALAAAGLVRAMRPLLAEYLPARDRARGRGEVVPGPGGPNDEARA"
     gene            1555..2310
                     /locus_tag="PLQ29_09680"
     CDS             1555..2310
                     /locus_tag="PLQ29_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG86957.1"
                     /translation="MKLELRNLVKTFADGTRGLDGASLSIETPSFVALSGQNGSGKSL
                     LIRHALGLEEADSGEVLVDGRPLHSRLAEARRRIALVFQEPEHQILGVTVREDVEFGP
                     RAAGERPESYAPRVEAALEAAGLAGYGPRLCAGLSGGEKRRLAVASALVSEPELIVLD
                     EPFNGLDWAGASDLLAVVLRLRASGVGVLVVTHDLEKCLAHADRLVVMDAGRIVRDDA
                     PAALWEELPSLGLRRPAGGYERLAEMSWLTEGA"
BASE COUNT          313 a          891 c         1000 g          444 t
ORIGIN      
        1 ccatgtgagg tttacgagat gatcggctcg tcgcctgagg cgactctgtc cccggctgag
       61 cgcgaccgct acatcgtcga gcggctgcgc gccgccggag agccactgtc gggagagtcc
      121 ctagcggcgg agctgggcgt tagccgggtg gcgctgtgga agcgcgtcgc ctcgctccgg
      181 gcttggggct acggcatcga agcttctcgt aggggctacg agctggtcag ggacgacggt
      241 ctggccggtt gggagctcga ggcgccgggg ccggtcgtgc tgttcgatac ggtcgagtcg
      301 acgatggacg aggcgcgggc gctggccgag gccggggctc cgtccggggc gacggtcctg
      361 gcgctcggcc agcgctcggg gcgcggccgt gccggcggcg cctgggatag tccggccggc
      421 ggcctctacc tgtcgacggc gctgcgctcg agcctaccgc catcccacgc cggggccctg
      481 ttcctcgagg cggccgccgc tacgctacat gccctcgagg ccgccggggc tccgggcctg
      541 tcgtactcgt ggccgagcga gctcaggcgc ggcggcgaga aggtcggcgg catactcgtc
      601 gaagcctacg gcgacatagc ctcggccgac tactacgtgg tcggcgtcgg cctgagcgtc
      661 gcgcccggcc tccttgccga agggtccacc gcgcggcgga cgtcggtggc ggccgcgctc
      721 gtgcgcgagc tggtcgcatg gtgcgccgcg ccgagccttg aactcggccg ttgggatagc
      781 ctggctccgc cggctggtag ccatatgtcc gtcgagctat ggaatggcga gcgccgcgcc
      841 ttcgaaccgg tcggcttcga tacgcgcggc gacctgaccg gcgccgacgg gacgcgcctg
      901 tcgatcggcg agtaccgctc gcatagcatt gaaggggaat atcaatgaaa cggaacggat
      961 tcaagctggt gctgtcgtcg ctgttcgccg cgatgatagc gatgggcgcg gtcttctcgc
     1021 tgcccttgcc gccgccgttg ccgccggtca cgctggcggt gttcttctcg ctactgtccg
     1081 ggctgctact ggggccgcgc tgggcgttag ccactaccgg cctctacctg gggctcggcg
     1141 cggtcgggtt gccggtgttc gccaatggag ccggcggcct cggtcagctg gcaggtccga
     1201 ccggcggctt cctcgtaggc tacgtcgcgg ccgccgtggt agccggagcc gtgtcggacc
     1261 ggcgtcgctg gggtttcgga cgggccgtgg ccggtgccct ggcaggtgtg gcggcgttgt
     1321 atatcgtcgg cctcccccga ttcagggccg tgctcgacgc cggtcccgac cgtagcgtga
     1381 cgatgggagc cgccttcctg atgatggcgc cgtacctggt aggcgatatg gctaaggcgc
     1441 tcgccgccgc cggactcgtg cgggctatgc ggccgctcct ggcggagtac ttgccggcgc
     1501 gggaccgggc gcgtggccga ggcgaggtgg tacccggccc gggagggccg aacgatgaag
     1561 ctcgagctta ggaacctggt caagaccttc gcggacggga cgcggggtct cgacggggcg
     1621 agcctatcga tcgagacgcc gtccttcgtc gcgctttcgg ggcagaacgg ctcgggcaag
     1681 tcgctgctga tccgccatgc gctcggcctc gaggaagctg actcgggcga ggtcctcgtc
     1741 gacggccggc ccctacatag ccgccttgcc gaggccaggc gccgcattgc cctcgtgttc
     1801 caggagcccg agcaccagat cctcggcgtg accgtccgcg aggatgtcga gttcggcccg
     1861 agagcggccg gagagcggcc cgagtcgtac gccccgcgcg tcgaggcggc gctcgaggcc
     1921 gcgggcctgg ccggctatgg cccgcgcctg tgcgccggcc tgtcgggcgg ggagaagcga
     1981 cggctggcgg tcgcctcggc cctggtatcc gaacccgagc tgatagttct cgacgagccg
     2041 ttcaacgggc ttgattgggc cggcgcctcc gaccttctcg cggtggtatt gcgcctacgc
     2101 gcctcaggtg tcggggtgct ggtcgtgact cacgacctgg agaagtgcct ggcgcacgct
     2161 gaccgcctgg tcgtcatgga cgccggccgt atcgttcgcg acgacgcccc tgccgcgctc
     2221 tgggaagagc tcccctcgct cggcctcaga cgcccggccg gcgggtacga gcggctggcg
     2281 gagatgagct ggctgacgga gggcgcgtga aggccgagcc atggcgcgtc ggcgtcgtcc
     2341 gcctggcggg ccgacgtccc ggcggaaggg tcggcgggat gatcgcgcgg actacgcgcg
     2401 tcacgcggac tacgcgcgtc acgcggacta cgcgcgccac gcggactacg cgcgccacgc
     2461 tagcggtacg cggtgtgtgg cgcttcgttc tatacttggc cctgcagctg gccatactcg
     2521 cttcgccgcc cacctggctg gccctgctgg ccgtcgtcgt ggcgcttctc ggcctggtcg
     2581 agggcaagga ctggccgcgc tggatcctac ggtcgcgctt cgcgctcgtg gcggccctcc
     2641 ttcccgcc
//
LOCUS       DAOPHV010000379         2644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_54433_length_2644_cov_4.230591, whole genome shotgun sequence.
ACCESSION   DAOPHV010000379 DAOPHV010000000
VERSION     DAOPHV010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54433_length_2644_cov_4.230591"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1824)
                     /locus_tag="PLQ29_09685"
     CDS             complement(<1..1824)
                     /locus_tag="PLQ29_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPG86958.1"
                     /translation="MAETLATMFMERVKARPELVMQYSKDGDGRFQPTSYAELLEHVA
                     TFAAGLLELGLRRGDRIGLISDNRREWLISDLAIIGLGAADVPRGCDATDQELAYILS
                     WSECAVAIVENDKQLAKLVARKREMPLLSTVVSFDPPSDGAAAAAKGAGFTNVDIDSD
                     GVRRRIKLVDFHDGKPYLQLSLAPLLDRLGKPGLVYGRRELRLIGAKYPDGVRDVRIP
                     LAMDGTMLIRWPKKTYIDSFDHIAFHDLLTYRDEAGAFADAVRLLRANQGWTLGPGYA
                     PVDACLAAWDESEAYRETALDSGSAEDREAWLASRREYWLTVGEFVGMDYGDSVPALY
                     DAARDADDPAYAELYDQMRDDFSLLWNRASESYARHLRYDEHLRGKLEGAFCIVGWTS
                     TGSTDFGVNPFDSEYENVGTHAAVANTILQRDFLREAPLWISSLLSLALAAAVLLVIG
                     RLGTRAQLLVGLGMTVAVALADYAVFHFTGVYVAVLSPLLATLVSFIAYSLLSFLLSE
                     REKSFLRKAFSTYLSGDVINEIISDPSMLKLGGQKKWITAMFTDIRGFSTISEALDAE
                     QLVKLLNIYLSGMSDIILENRGTIDKYEGDALMAEFGAPLPD"
     gene            complement(1915..>2644)
                     /locus_tag="PLQ29_09690"
     CDS             complement(1915..>2644)
                     /locus_tag="PLQ29_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HPG86959.1"
                     /translation="AAGSCLGGLDANFGCSAPLIEKAGGGVSWMKDADRSARLIAALK
                     TAAPGVPVSAKLRLGYEASEAALIAFCRALEAAGADYIVVHPRLKHEKFRRSGHWEFV
                     AAVSTAVGIPVIGNGDIRDAGGYRTVMERYGATGAMLGREAVRRPWIFSLIRGKEANP
                     AFGMSVAIEETGLRMIALIRELLPSPLHISRARRFFFYYCDNLSFGHHLRWSIQNADG
                     LDEVERLFRAYFDEVPSDRVRLER"
BASE COUNT          465 a          841 c          875 g          463 t
ORIGIN      
        1 gtcgggaagg ggcgcgccaa actccgccat gagcgcgtcg ccctcgtact tgtctatagt
       61 gccccggttc tcgaggatga tgtcgctcat gccggacagg tagatattga gcagcttgac
      121 gagctgctcg gcgtccaagg cctcggaaat ggtcgagaag ccgcggatgt cggtgaacat
      181 cgcggtaatc cattttttct ggccgccgag cttgagcatc gacgggtcgg agatgatctc
      241 gttgatcacg tcgccggaca ggtaggtgct gaaagccttg cgtaggaagc ttttctcccg
      301 ctcggaaagg aggaacgata gcagggagta ggcgatgaac gacaccaggg tggctaggag
      361 tggcgacagg acggcgacgt agacgcccgt aaaatggaat acggcgtagt cggccagggc
      421 gacggcgacc gtcatgccca agccgaccag cagctgcgcc cgtgtgccga gcctgccgat
      481 aaccagcaat accgcggccg cgagggcgag cgataatagc gacgatatcc acagcggcgc
      541 ctcccgtagg aagtcgcgct gcagtatcgt gttggcgacc gcggcatgcg tcccgacgtt
      601 ctcgtattcc gagtcgaagg gattgacgcc gaaatcggtc gagccggtac tggtccagcc
      661 gacgatgcag aacgcgcctt cgagcttgcc gcgcaggtgc tcgtcgtagc gcaggtgcct
      721 ggcgtaggat tccgaggccc ggttccagag gagggagaag tcgtcgcgca tctgatcgta
      781 cagctccgcg taggccgggt cgtcggcgtc ccgggccgcg tcgtacaggg cggggacgct
      841 gtcgccgtag tccataccga cgaactcccc gaccgtcagc cagtattccc gcctggaggc
      901 cagccaggcc tcgcggtcct cggccgaccc gctgtcgagt gcggtctcgc ggtaggcttc
      961 gctctcgtcc caggccgcca ggcacgcgtc gaccggcgca tagcccggcc cgagggtcca
     1021 gccctggttg gccctgagca gcctgacggc gtcggcgaac gccccggctt catcgcggta
     1081 ggtcagcagg tcgtggaacg cgatgtggtc gaagctgtcg atatacgtct tcttgggcca
     1141 gcggatcagc atcgtgccgt ccatggccag cgggatgcgt acgtcgcgta cgccgtccgg
     1201 gtacttcgcg ccgatcagcc gcagctcgcg ccggccgtag acgaggccgg gcttaccgag
     1261 cctgtcgagg aggggcgcca gggacagctg taggtagggc ttgccgtcgt ggaagtcgac
     1321 cagcttgatg cgcctacgca cgccgtccga gtcgatatcg acgttcgtga agcccgcgcc
     1381 cttggccgca gcagcggcgc catccgacgg agggtcgaac gatacgaccg tcgacaggag
     1441 cggcatttcc cgcttcctgg ccaccagctt ggccagctgc ttatcgttct ccacgatggc
     1501 cacggcgcac tcgctccacg acaggatgta ggccagctcc tggtcggtcg cgtcgcaacc
     1561 gcgcggtaca tcggccgcgc cgagcccgat gatggctagg tcgctgatca gccactcgcg
     1621 gcggttgtcg gagatgagtc ctatccggtc gcctcgcctc aggcccagtt cgagcagccc
     1681 ggcagcaaag gtcgccacgt gctcgagcag ttccgcgtag cttgtcggct ggaagcggcc
     1741 gtcgccgtcc ttgctgtact gcatcacgag ctcgggtctc gccttgaccc gttccatgaa
     1801 cattgtcgcg agcgtctcgg ccatggtagc ctcctcttca gcttcttttg acaatatact
     1861 gaaaagtgta aaaagacgca agggttgaaa agtgtcggcg aaaaccttct acgctcagcg
     1921 ctccagcctg acgcgatccg acggcacctc gtcgaagtag gccctgaaca ggcgctcgac
     1981 ctcgtccagg ccgtcggcgt tctggatgga ccagcggagg tgatggccga acgacaggtt
     2041 gtcgcaatag tagaagaaga aacgcctggc ccggctgata tggagcggcg agggtagcag
     2101 ctcccggatc aaggctatca tcctcaggcc cgtctcctcg atggccacgc tcatgccgaa
     2161 ggccggattc gcttctttac cgcggatgag cgagaatatc cagggccgac ggacggcctc
     2221 tcggccgagc atagcgcccg tggctccata gcgctccatg acggtcctat acccgcccgc
     2281 atcgcggatg tcgccgttgc ctatcactgg tatgccgacg gccgtcgaga cggcggccac
     2341 gaactcccag tgcccggaac gcctgaactt ttcgtgcttg agcctggggt ggacgacgat
     2401 gtagtcggcg ccggcggctt cgagcgcccg gcagaaggct atcagcgccg cttcggaggc
     2461 ctcgtaaccg agccgtagct tggccgagac cggaacgccc ggagcggcgg tcttgagggc
     2521 cgcgatcagg cgggccgacc ggtctgcgtc cttcatccag cttaccccac cgccggcctt
     2581 ctctatgaga ggggccgagc acccgaagtt ggcgtccagg ccgccgaggc acgagccggc
     2641 ggcc
//
LOCUS       DAOPHV010000380         2643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_54461_length_2643_cov_4.205178, whole genome shotgun sequence.
ACCESSION   DAOPHV010000380 DAOPHV010000000
VERSION     DAOPHV010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54461_length_2643_cov_4.205178"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1675
                     /locus_tag="PLQ29_09695"
     CDS             <1..1675
                     /locus_tag="PLQ29_09695"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPG86960.1"
                     /translation="RSFITKSRVYRQHLAVMAAGLLFSVAWGYVFAIVGPTGILKAAY
                     PLSAVNTLVAVLAASYAFSSTRVFQPRLVAKTFGVWLAIIVAFALPLGAVVAVAFLFR
                     AEAGATALGIAAIAFLLLGRGAESFARGRMGSEHDAASREDLEAAIAHLDLSAGRESV
                     LGELSAIVGESFGCSWLAVLSEDDAGNLRRIYPDDDDEIVTTPNSPALEALAAVDRRV
                     ILKTDVVADASFAELKPVLLGFFDVLGAEAIVIAKEGRRVVGLFALGPKRSGADYDAQ
                     DYAAFDAVHGKLFVVAYYARHVARESLLGTVEKEIGLADQIVKSIQEKIDPIDHPGVE
                     VSFVCQSPRGLGGDLFDSVRISEHRWFFVVGDISGKGLNASMSMVILKSMIRTLLKEE
                     KDFIKLVARANVFIKERLPRGTFFSGMFGFLALDKGSIYFINCGIPAMFFRSPGLDSV
                     IETQGEGKMLGFVRNVEPYLKTRKRALPPGSRVLISTDGIVEAESVRGERYGKERLVR
                     ILAENKDASAAETVGAVVKSASAFAGGRLDDDITVVAIDYAGIPKEKKR"
     gene            1672..2001
                     /locus_tag="PLQ29_09700"
     CDS             1672..2001
                     /locus_tag="PLQ29_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPG86961.1"
                     /translation="MTGISVEAMERGAWTVLSVHGPINSYTFNELQEKTMLHAGRVDM
                     AVDLSGVSSMTSAGIGVLLAAIEDAETAGKRFFVIAPSEVSRLAIESTGFADRFPTLP
                     SIKELPV"
     gene            2017..2523
                     /locus_tag="PLQ29_09705"
     CDS             2017..2523
                     /locus_tag="PLQ29_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86962.1"
                     /translation="MPLIAPPSRRRTPQESLGAAPRARSRIRLSPGGTIFDEYDGFIR
                     SLGFLNGREAAKLIVAGDEVLDNLMTHGEISAAGVCVLVTRRERSGLTLGFFVDSHRE
                     FAEFTRHADERDGARPRFDQRERRWHGLGLTMCRNIASSVSYRSGELVDRVFLSFDVE
                     PRPARSAP"
BASE COUNT          419 a          870 c          880 g          474 t
ORIGIN      
        1 gcggtcgttc atcacgaaga gccgcgtcta tcgtcagcac ctcgccgtga tggccgcggg
       61 cctcctgttc agcgtggcct ggggctacgt cttcgcgatc gtcgggccga cgggcatcct
      121 caaggcggcg tatccgctgt ccgcggtcaa tacgctcgtg gcggtgctcg ccgcctcgta
      181 cgccttctcg tcgactcgcg tgttccagcc caggctcgtg gccaagacct tcggcgtgtg
      241 gctggccatc atcgtcgcct tcgcgctacc gctcggagcg gtcgtggccg tagcgttcct
      301 gttcagggcc gaggccggag cgacggccct gggcatcgcc gccatcgcct tcctactcct
      361 cggcaggggc gccgagtcgt tcgcgcgcgg taggatgggc tcggagcacg acgccgcctc
      421 ccgggaagac ctcgaggccg ctatagcgca cctcgacctg tcggccggcc gagaatcggt
      481 gctcggagag ctttcggcca tcgtcggcga gtcgttcggc tgctcgtggc tcgccgtgtt
      541 gtccgaggat gacgctggca acctgcggcg catctacccg gacgacgacg acgagatcgt
      601 aacgaccccg aattcccccg cgctcgaggc cctcgctgcg gtcgataggc gcgtgatact
      661 caagacggac gtggtggctg acgcctcttt cgcggagctc aagcccgtgc tgctcggttt
      721 cttcgacgtg ctcggcgccg aggccatcgt gatagccaag gaaggccgtc gcgtcgtcgg
      781 cctgttcgcc ctcggaccga agcgctccgg cgccgactac gacgcccagg actacgccgc
      841 cttcgacgcg gtacacggca agctcttcgt cgtcgcctac tacgcccgcc acgtggcgcg
      901 cgagtccctg ctcggcacgg tggagaagga gatcggccta gccgaccaga tcgtcaagtc
      961 gatccaggag aagatcgacc ccatcgacca cccgggcgtc gaggtgtcct tcgtctgcca
     1021 gtcgccgcgc ggcctcggcg gcgacttgtt cgactcggtg cgcatctcgg aacatcgctg
     1081 gttcttcgtc gtgggcgaca tctccggcaa gggcctcaat gccagcatgt cgatggtcat
     1141 cctcaagtcg atgatacgca cgcttctcaa ggaggagaag gactttatca agctggtggc
     1201 gagggcgaac gtctttatca aggaacgcct gccgcgcggt accttcttct cgggcatgtt
     1261 cggcttcctc gccctcgaca aggggtcgat ctacttcata aactgcggca tcccagccat
     1321 gttcttccgc tcccccggcc tcgactcggt gatcgagacc cagggcgagg gcaagatgct
     1381 cggcttcgtc aggaacgtcg agccctatct gaagaccagg aagcgggcct tgccgcccgg
     1441 ttcgcgcgtg ctgatcagta cggacggcat cgtcgaggcc gagagcgtgc gcggcgagcg
     1501 atacggtaag gagcgcctgg tgcgtatctt ggcggagaac aaggacgcga gcgccgccga
     1561 gacggtggga gcggtcgtca agtcggcgag cgccttcgcc ggcgggcggc tggacgacga
     1621 tataacggtg gtggccatcg actacgcggg catacccaag gagaagaagc gatgacagga
     1681 atcagcgtcg aggcgatgga acgcggcgcg tggaccgtat tgtcggtgca cgggcccatc
     1741 aatagctata cgttcaacga gctgcaggag aagacgatgc tgcacgcggg ccgggtggac
     1801 atggcggtcg acctctcggg cgtcagctcg atgaccagcg cgggtatcgg tgtgctgctc
     1861 gcggccatcg aggacgcgga gaccgcgggc aagcgtttct tcgtgatcgc gccgtccgag
     1921 gtctccaggc tcgctatcga gtccacgggt ttcgcggatc gtttcccgac cctgccatcg
     1981 atcaaggagc taccggtcta gcccggaccg ttccctatgc ccttgatcgc gcctccgagc
     2041 cgacgacgaa cgcctcagga atcgctcggg gccgcgccgc gcgccaggtc gaggatcagg
     2101 ctgtcccccg gcggcaccat cttcgacgag tacgacggtt ttatccgatc ccttggcttc
     2161 ttgaacgggc gcgaggcggc gaagctgatc gtcgccggcg acgaggtgct cgacaacctg
     2221 atgacgcacg gcgagatcag cgccgccggc gtctgcgtac tggtcactcg gcgggagcgg
     2281 agcgggctga ccctgggctt cttcgtggac agccatcgcg agttcgccga gttcaccagg
     2341 cacgccgatg agcgcgacgg cgcgaggccg cgcttcgacc agcgggagcg ccgctggcac
     2401 ggcctcggcc tgaccatgtg ccggaatatc gcctcgagcg tcagctatag gtccggagag
     2461 ctcgtcgacc gggtgttcct gagcttcgac gtcgagccta ggcctgcccg gtcagcgcct
     2521 tgatgtagtg caggcgtccg aacagttccg gcgcgtccga attcctgcgc gccagccttc
     2581 ccctcagctc cgatagcctc gcaatgccgt gggcgtcgag ccaggagccc atctcctcga
     2641 tgg
//
LOCUS       DAOPHV010000381         2640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_54561_length_2640_cov_2.736557, whole genome shotgun sequence.
ACCESSION   DAOPHV010000381 DAOPHV010000000
VERSION     DAOPHV010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54561_length_2640_cov_2.736557"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLQ29_09710"
     CDS             complement(<1..218)
                     /locus_tag="PLQ29_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPG86963.1"
                     /translation="MVVKVDHIGIAVSNLEETLKVYTDLLGMQLHGTETVEEQKVKTA
                     FLPVGDSEIELLESTAPDGPIAKFIETRG"
     gene            complement(227..1354)
                     /gene="meaB"
                     /locus_tag="PLQ29_09715"
     CDS             complement(227..1354)
                     /gene="meaB"
                     /locus_tag="PLQ29_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPG86964.1"
                     /translation="MDDSAVSADRVRAGDARATARLISMIEREDPSRLAVLKALHAYG
                     GKAHVVGITGPPGAGKSTLTDKLVGDLRGRGRKVAVIAVDPSSPFTGGAILGDRIRMQ
                     RHATDDGVYIRSLATRGHLGGLSRAASEAVRVLDAAGYDDVIIETVGVGQSEVDIVRI
                     ADTVVLVSVPGLGDDIQAIKAGVMEIGDVFCVNKADKDGAERVVREIRAMLETAVLNG
                     AATRFTPLFEARSALRGDAGNAGGATEAVASRGPAEQGAFHHITAQATGHPNLEIPPV
                     VKTVAETGEGIGPLVDEIKAHEAALAATGFLRSRREANLAWELGRGAAQRVLSELERD
                     ESRETLAELARSVLDRGIDYYDALDALEDAMKRTQRAATTA"
     gene            complement(1358..1759)
                     /locus_tag="PLQ29_09720"
     CDS             complement(1358..1759)
                     /locus_tag="PLQ29_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HPG86965.1"
                     /translation="MERTIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTGLRQTP
                     EQIVSAAVQEDVDVVAMSILSGAHNHLFPRVMELLKEKGVDDVLVIGGGVIPDDDIPG
                     LKGAGIAAVFTPGSPTGDIIEFIKKNLKRKA"
     gene            complement(1772..>2640)
                     /locus_tag="PLQ29_09725"
     CDS             complement(1772..>2640)
                     /locus_tag="PLQ29_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPG86966.1"
                     /translation="KAGLDVDQFAPRLSFFFNAHNDLFEEVAKFRAARRVWAKVMKER
                     FGAKSPKSLSLRFHTQTAGSTLTAQQPDNNIVRVAIQTLAAVLGGTQSLHTNSKDEAL
                     ALPTEASVRIALRTQQIVAYESGAAETIDPLAGSYYVESMTDRIQKEAEAYIATIDGL
                     GGAVKAIEKGYVQQEIQDAAYAYQMDVESGERVVVGVNKFQIEEGSPKGLLRVDPIVG
                     ERQAAKLKELRAKRDNRAVEGTLAALKAGAKGDANLMPLILDAVKAYATLGEICDVLR
                     AEFGEYQQKVIL"
BASE COUNT          367 a          888 c          890 g          495 t
ORIGIN      
        1 ccccgggtct cgatgaactt ggctatcggg ccgtccgggg cggtcgactc gagcagctct
       61 atctccgagt cgccgaccgg caggaatgct gtcttgacct tctgctcctc gacggtctca
      121 gtgccgtgca gctgcatgcc caagaggtcg gtatacacct tgagcgtctc ttccaggttc
      181 gatacggcaa tgccgatgtg gtcaaccttg acgaccatga atcctcctat gctgtcgtcg
      241 ccgcgcgctg ggtacgcttc atcgcgtcct cgagcgcgtc gagcgcgtcg tagtagtcta
      301 tgccccggtc gaggaccgac cgggccagct cggccagggt ctcgcggctc tcgtcccgct
      361 cgagttccga tagcacccgc tgggctgctc cgcgaccgag ttcccaagcc aggttggcct
      421 cgcgcctcga gcgcaggaag cccgtggcgg cgagagccgc ctcgtgcgcc ttgatctcgt
      481 cgaccagggg cccgatgccc tcgcccgtct cggccaccgt cttgacgacc ggcggtatct
      541 cgaggttcgg gtggccggtc gcctgcgcgg ttatatggtg gaaggcgccc tgctccgccg
      601 ggcctctgga cgcgacggcc tccgtcgcgc cgcccgcatt gccagcgtcg ccgcgtagcg
      661 ccgagcgcgc ctcgaatagc ggcgtgaagc gggtcgcggc cccgttgagc acggcggtct
      721 cgagcatcgc gcgtatctcg cgtaccacgc gctcggcgcc gtccttatcg gctttgttca
      781 cgcagaagac gtcgcctatc tccatgacgc cggccttgat cgcctgaatg tcgtccccga
      841 ggccggggac cgatacgagc acgacggtgt cggctatgcg cacgatgtcc acctcggact
      901 ggccgacgcc gacggtctcg atgatcacgt cgtcgtagcc ggcagcgtcg aggacgcgca
      961 cggcctcgct ggcggccctg gataggccgc cgaggtggcc gcgcgtggcc aagctacgga
     1021 tatagacgcc gtcgtcggtc gcgtggcgct gcatacggat gcggtcgccg agtatggcgc
     1081 cgccggtgaa cgggctggac gggtctacgg cgatgaccgc caccttgcga ccgcggccgc
     1141 gcaggtcacc gaccagcttg tcggtcagcg tactcttacc ggcgcctggc ggcccggtga
     1201 tgccgacgac gtgggccttg ccgccgtagg catggagcgc cttgaggacg gcgaggcgcg
     1261 acggatcctc ccgctcgatc atggagatca ggcgggccgt cgccctcgcg tcgccggcgc
     1321 ggacccggtc cgcgcttacg gcagaatcgt ccacggccta cgccttccgc ttgaggttct
     1381 tcttgatgaa ctcgatgata tcgccggtcg gcgagcctgg ggtaaagacg gccgcgatac
     1441 cggcgccttt gaggccgggg atatcgtcgt ccgggatcac gccgccgccg atcacgagca
     1501 cgtcgtcgac gcccttctcc ttgagcagct ccatgacgcg tgggaacagg tggttatgcg
     1561 cgccggagag gatgctcatc gccacaacgt cgacgtcctc ctggacggct gccgagacga
     1621 tctgctccgg cgtctgtctg aggcccgtat agatcacttc catgccggcg tcgcgcagcg
     1681 ccctggctat caccttggcg ccgcggtcgt ggccgtcgag gcccggcttg gctacgagta
     1741 cgcgtatcgt tcgttccatg tccacctacc tttacaggat gaccttctgc tggtactcgc
     1801 cgaactcggc acgcagtaca tcgcagatct cgccgagggt cgcgtaggcc ttgacggcgt
     1861 cgaggatgag cggcatcagg ttggcgtcgc ccttggcgcc cgccttgaga gcggccagcg
     1921 taccctcgac ggcccggttg tcgcgcttgg cccgcagctc cttgagcttg gcggcctgcc
     1981 tctcgccgac gatcgggtcg acccgcagga ggcccttggg cgagccttcc tcgatctgga
     2041 acttgttgac gccgaccacg acgcgttcgc ctgactccac gtccatctgg tacgcgtagg
     2101 ccgcgtcctg gatctcctgc tggacgtagc ctttctcgat agccttgacc gcgccgccga
     2161 ggccgtcgat cgtggcgatg taggcctccg cttccttctg gatgcggtcg gtcatgctct
     2221 ccacgtagta cgagccggcg agcgggtcga tggtctccgc ggcgcccgac tcgtaggcga
     2281 cgatctgctg ggtacgcagc gcgatgcgca cagaggcctc ggtcgggagc gcgagcgcct
     2341 cgtccttgga gttggtgtgc agggactggg tgccgccgag cacggcggcc agggtctgga
     2401 tcgccacgcg gacgatgttg ttatcgggct gctgggccgt cagcgtggag ccggcggtct
     2461 gcgtatggaa gcgtagcgac aggctcttcg ggctcttggc gccgaagcgc tccttcatca
     2521 ccttggccca gacgcggcgg gcggcccgga acttggccac ttcctcgaag aggtcgttgt
     2581 gggcgttgaa gaagaacgac aggcgcggcg cgaactgatc gacgtcgagt ccagccttga
//
LOCUS       DAOPHV010000382         2637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_54622_length_2637_cov_4.080558, whole genome shotgun sequence.
ACCESSION   DAOPHV010000382 DAOPHV010000000
VERSION     DAOPHV010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54622_length_2637_cov_4.080558"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1079)
                     /gene="trxB"
                     /locus_tag="PLQ29_09730"
     CDS             complement(126..1079)
                     /gene="trxB"
                     /locus_tag="PLQ29_09730"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPG86967.1"
                     /translation="MAEATQMEQYDLVIVGAGAAGLGAAQYGARANLKTLVIEEMAHG
                     GQALLIDKLENYPGIPEPVDGYTWAETMRAQAVDFGASIMSSSVASVRKDGERFVVET
                     GDGPVGAKAVVIATGAKHRHLGVKGEEEFAGRGVSYCATCDGPFFRGKRMIVVGGGDS
                     ACDEAMFLSKLADKVVMVHRKDRFRAQKAVAARVLSNPKIEVRFETVLDEIKGTKAVE
                     AAVLRNLSSGEVEELPTSAVFVFVGSIPQVDALPPEVAKDGSGSIVTNDRMETNVPGL
                     FAAGDVRATPFRQVVTAVSDGAIAAHCAAQYIDELEGKAYL"
     gene            1120..2451
                     /locus_tag="PLQ29_09735"
     CDS             1120..2451
                     /locus_tag="PLQ29_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86968.1"
                     /translation="MGICHSRGSGATTRRIVDALRDFGVATALVLAAAGAPSPSAEPV
                     LPSPSAEVLALAAGPEAPTAAELLAAALLFSDAARADAARADAARADAAMAAGLEAIA
                     AAEALAASVAGQYELGAALLAAMHARLSEYRLMESRLDRALLEGRYNCVGSSTLYAIM
                     ASAAGLRVRGVLLDDHAYCYLETAGGRVDVETTIPGGYDARKGEPPAGAADVSVRGVI
                     ALALRNRATTLDRSGQWDGALALAVDAYAYAPDPSSFSTLVGRVNNAVAYRIRSGAYG
                     EALGLAEAAQLAYGPDRRLDEIRRSAALAAATEALRVAKPDEALALADGVAASGIADE
                     AWLERAYAYAYAGLAERLRAAGDHLGAWALASEAVARFPAYGALSSLERTARINWARA
                     EHNRFAALYNAGAYGEALAVVRAALALAPGDAMLLADEKAAAEAIGPGQRP"
     gene            complement(2426..>2637)
                     /locus_tag="PLQ29_09740"
     CDS             complement(2426..>2637)
                     /locus_tag="PLQ29_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG86969.1"
                     /translation="FSVSDDGAGFRTVYRGHGEPPPAGKNKGIGMDLVEALAAQLGGR
                     MERGSPPAGAVTTVRFPVRDAAQDR"
BASE COUNT          361 a          948 c          919 g          409 t
ORIGIN      
        1 tccgcgactc agcgagtacg agaagggtat ggccgcgccc accgcggcga ccacggccag
       61 cgccgcgatg ccgaggcgcc aggctcgagc gtaacgggtc ggttcggggg ccatgccgct
      121 tcggcctaga ggtaggcctt gccctccagc tcgtcgatgt actgggccgc gcagtgcgcg
      181 gctatggcgc cgtcggagac ggcggtgacg acttgcctga acggcgtcgc gcggacgtcg
      241 ccggccgcga agaggcccgg cacgttcgtc tccatgcggt cgttcgtcac aatggagccg
      301 ctaccgtcct tggccacctc ggggggcagg gcgtcgacct gcgggatcga gcctacgaac
      361 acgaagaccg ccgaggtcgg cagttcctcg acctcgccgg aactcaggtt cctgagcacg
      421 gcggcctcga cggccttggt tcccttgatc tcgtcgagca cggtctcgaa tcggacctcg
      481 atcttcgggt tcgacaggac cctggccgcc acggctttct gggcgcgaaa gcggtccttg
      541 cggtggacca tgacgacctt gtcggccagc ttcgacagga acatcgcctc gtcgcaggcc
      601 gagtcgccgc caccgaccac gatcatgcgc ttgccccgga agaaggggcc gtcgcaggtg
      661 gcgcagtatg atacgccgcg gccggcgaat tcctcctcac ccttgacgcc gaggtggcga
      721 tgcttggccc cggtcgcgat cacgacggcc ttggctccta ccggcccgtc gccggtctcg
      781 acgacgaagc gctcgccgtc cttgcggacc gaggcgacgc tggacgacat gatggaggcg
      841 ccaaaatcga cggcctgggc ccgcatcgtc tccgcccagg tatagccgtc cacgggctcg
      901 gggatgccgg gatagttctc cagcttgtct atgagcaagg cctgtccgcc gtgggccatc
      961 tcctcgatga cgagagtctt caggttggcg cgcgcgccgt attgcgccgc tccgagccca
     1021 gcggcccccg ctcccactat gacaaggtcg tactgttcca tctgcgttgc ctccgctatc
     1081 gtagcgcgtg attttacgat gatttataat acccatacca tgggtatttg tcactctcga
     1141 ggatctggag cgacgacgag gcgcatcgtc gacgcgcttc gcgatttcgg cgtcgccacg
     1201 gctctcgtcc tggccgccgc gggtgcgcct tcgccgagcg ccgagcccgt cctgccttcg
     1261 ccgagcgccg aggtcctggc gctggcggcc ggacccgagg cgccgacggc cgccgagctg
     1321 ctcgccgccg ccctgctgtt ctctgacgcc gcgcgcgccg acgccgcgcg cgccgacgcc
     1381 gcgcgcgccg acgccgccat ggcggccgga ctagaagcga tagcggccgc cgaggccctg
     1441 gccgcctcgg tggccggcca gtacgagctc ggggcggcgc tgctcgccgc catgcatgcg
     1501 cgcctatccg agtaccggct catggagtcg cgcctggacc gcgcgctgct tgaggggcgg
     1561 tataactgcg tcggctcgtc gacgctgtac gccattatgg cgagcgccgc aggcctgcgc
     1621 gtccggggcg tgctgctcga tgaccatgcc tactgctacc tcgagacggc cggcggccgc
     1681 gtcgacgtgg agaccaccat ccccggcggt tacgatgccc gcaagggcga gccgcccgcc
     1741 ggcgcggccg acgtgtcggt ccgcggcgtc atcgcgctgg ccctgcgtaa ccgcgcgacg
     1801 acgctcgatc gctccgggca atgggatggg gcgctcgctc tggcggtcga cgcctacgcc
     1861 tacgcgcccg acccgtcatc gttctcgacc ctcgtgggaa gggtcaacaa cgcggtggcg
     1921 taccgtatac ggagcggtgc ctacggcgaa gccctgggcc tggccgaggc ggcccagctg
     1981 gcgtacggcc cggaccgacg cctggacgag atccgccgaa gcgcggccct cgccgcggcg
     2041 accgaggcgc tacgggtcgc gaagcccgac gaggccctcg ccctggccga cggggtggcc
     2101 gcctccggta tcgcggacga ggcctggctc gagcgcgcct atgcctacgc ctacgccggc
     2161 ctcgccgagc ggctacgggc cgcgggcgac catctcggcg cctgggcctt ggcttccgag
     2221 gcggtagcgc gtttcccagc gtacggggcc ctctcctcgc ttgaacggac ggccaggatc
     2281 aactgggcga gggccgaaca taaccgcttc gcggcgctgt acaacgccgg agcctacggc
     2341 gaggccctgg ccgtcgtgag ggcggccctc gccctggccc cgggagacgc gatgctgctg
     2401 gccgacgaga aggcggccgc cgaggctatc ggtcctgggc agcgtccctg acggggaatc
     2461 ggaccgtggt tacggcgccc gccggcggag agccccgctc catgcggccg cctagctggg
     2521 ccgccagggc ctcgaccagg tccatgccta tgcccttgtt cttgccggcc ggagggggct
     2581 cgccgtggcc gcgatacacg gtccggaagc cggcgccgtc gtccgatacc gagaacg
//
LOCUS       DAOPHV010000383         2632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_54777_length_2632_cov_4.198681, whole genome shotgun sequence.
ACCESSION   DAOPHV010000383 DAOPHV010000000
VERSION     DAOPHV010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54777_length_2632_cov_4.198681"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1850)
                     /locus_tag="PLQ29_09745"
     CDS             complement(<1..1850)
                     /locus_tag="PLQ29_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPG86970.1"
                     /translation="MKTKRTLLIATCALTLWSCVSSPKPAGEPAAQAPAPAAEPSIAE
                     LIEAGDAAGLKALFKGREKASQPAADGRYPLHAAVLKGSAEMVEILLAMGAEPDPKDA
                     EGKTPLRYAIDAMDTRSAKALIAKGASVFSIDAAGVSPLDAAIAKGFVASVVDSLSVS
                     AKGPKGETPLHTAVDRLSIEAVKALLALGPDLAARDGSGRTALDAAFLHPGNIEGAAI
                     AELLVSRNAPSSIDDFAYFIRAVRDTNYTRARFADGATVLHEAVRYDHRGYLSFFLER
                     GVPVDAKNASGATALHDAVRLGRLDAARLLLARKADPNAVDGSGDAPLHIALPGSAGQ
                     VAVDLLLDAGASTSIKNRAGNTALHLAVALGYGQPMIERLIAAGSPVDAANADGDTAL
                     ALAMRRRNEALATTLAGRGASMYVKNLKGETPLSIALADGPSSTRILVAASPKGAKDE
                     SGDSPYHHATRLRSDPGDIAVLSELGLDPSARNNEGDTALHLAARLRAEAQGLALLAA
                     GSDPLLANAAGATPLTTALTASGGPLVWFFTPEVLAAEDPSGNGPLHHAAMAGLADGA
                     AYLARLGVPVDGKNDDGQTALMLALKRDSTGTVNALLALGADPGIRDSSGS"
     gene            1992..>2632
                     /locus_tag="PLQ29_09750"
     CDS             1992..>2632
                     /locus_tag="PLQ29_09750"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPG86971.1"
                     /translation="MRIGFCVPMNKAWSAAADGIEREHGEKVELVRGVEACLEAFPSL
                     DAVIANRVDASSYGAARSLKAVFVPFVGVNHLPADLLIERGVAVFNCHGNAESVAQHA
                     LSLALAGFGRLIEFHNDLRAETWHGFWVDKGSEDFWHSIYRKRCAILGAGAIGQKLAR
                     LLKAFDCTVIGYRRRPGEPMPECFDEVQGDLKAAVTGAEIVFVTLPLTDATRGL"
BASE COUNT          397 a          845 c          943 g          447 t
ORIGIN      
        1 gagccggacg agtcgcgtat accggggtcg gccccgagcg cgaggagcgc gttgacggtg
       61 cctgtcgagt cgcgtttgag cgccagcatc agggccgtct ggccgtcgtc gttcttgccg
      121 tccaccggta cgcccagcct cgccaggtag gccgcgccgt cggccaggcc tgccatcgcc
      181 gcgtgatgga ggggtccgtt acccgacggg tcctcggcgg ccaatacctc tggcgtgaag
      241 aaccatacga gcggcccgcc cgacgccgtg agcgccgtcg tgagcggcgt ggctccagcc
      301 gcgttggcga gcaacgggtc cgatcctgcc gctagtaacg cgagaccctg ggcctcggcg
      361 cgcaggcggg cggccaggtg caaggccgtg tcgccctcgt tgttgcgagc cgacgggtcg
      421 agcccgagct cggacaggac ggctatatcg cccgggtcgg atcgtagcct cgtcgcgtga
      481 tggtagggcg agtcgcccga ctcgtccttg gcgcccttgg gcgacgcggc gacgaggatg
      541 cgcgtcgacg agggcccgtc ggcgagcgct atcgatagcg gcgtctcgcc cttgaggttc
      601 ttcacgtaca tactggcgcc ccgaccggcc agcgtcgtcg ccagggcctc gttccgacgg
      661 cgcatcgcca gggccagggc cgtatccccg tcggcgttgg cggcatcgac gggcgagccc
      721 gccgctatca gccgttcgat catcggctgg ccgtagccga gggcgaccgc caggtggagc
      781 gccgtattgc cggcccggtt cttgatcgac gtggaggcgc cggcgtcgag cagcaggtcg
      841 acggcgacct ggcctgcaga cccgggcaag gctatatgga gcggggcatc gccggagccg
      901 tcgacagcgt tcgggtctgc cttcctggcg agcagtaacc tggccgcgtc gagtcggccg
      961 agtctgaccg cgtcgtgcag ggccgtggcc cccgaggcgt tcttcgcgtc gacgggcacg
     1021 cctcgctcaa ggaagaacga caggtagcct cgatggtcgt agcggacggc ctcgtggagc
     1081 acggtcgccc cgtcggcgaa gcgggcgcgg gtataattcg tgtccctgac ggcacgtatg
     1141 aaataggcga agtcgtcgat cgagctgggc gcgttcctcg ataccaagag ctcggctatc
     1201 gcggcgccct ctatgttgcc tgggtgtagg aaggccgcgt cgagggctgt cctaccggag
     1261 ccgtcgcggg cggcgaggtc ggggccgaga gccaggaggg ctttgaccgc ctcgatggac
     1321 agcctgtcga cggcggtatg gagcggcgtc tcgcctttag ggcctttggc cgagacggag
     1381 agcgagtcga cgaccgaggc gacgaagccc ttggctatgg ccgcgtcgag cgggctcacg
     1441 ccggcggcgt cgatggagaa gaccgaggcg cccttggcga tgagcgcctt ggcggacctg
     1501 gtgtccatcg cgtcgatcgc gtagcgcagg ggcgtcttgc cctcggcgtc cttggggtcg
     1561 ggctcggcgc ccatcgcgag caatatctct accatttcgg ccgagccctt gagcaccgcc
     1621 gcgtggaggg gatagcggcc gtcggcagcc ggctgactgg ctttctccct gcccttgaac
     1681 agggccttga gaccagccgc gtctccggcc tcgataagct cggctatgct cggctcggcc
     1741 gcgggagccg gcgcttgagc cgccggttcc ccggcgggct tgggagagga cacgcacgac
     1801 cagagagtca gagcgcaggt cgctataagg agcgtacgct tggttttcac agtctattcc
     1861 ttccagagta tagtgtatcg cgtcagaggc cgcggggcga taggctttct ttttatcatc
     1921 ggaatcgcgt atcctatgct tgaaccacta gatcggcgtc cgcgtagccg ggcgccaacg
     1981 gaggcgacag cgtgaggatc ggattctgcg tacctatgaa caaggcctgg tcggccgccg
     2041 ccgacggcat agagcgggag cacggagaga aggtcgaact ggtacggggc gtcgaagcct
     2101 gcctcgaggc gttcccgagc ctcgacgcgg tgatcgcgaa ccgtgtggat gcctcgagct
     2161 acggggcggc tcggtcgctc aaggccgttt tcgtgccctt cgtcggcgtc aaccatctcc
     2221 ccgccgacct gctgatcgag cgcggcgtgg cggtgttcaa ctgccacggc aacgcggaga
     2281 gcgtggctca acacgcgctg tcgctggcgc tcgcgggctt cggcaggctg atcgagttcc
     2341 ataacgacct gcgggccgag acctggcatg gcttctgggt ggacaagggc agcgaggatt
     2401 tctggcattc catctacagg aagcgctgcg ccatactcgg cgccggtgcg ataggacaga
     2461 agctcgcgcg cctgctcaag gccttcgact gcacggtaat cggctatcgc cgtaggcccg
     2521 gcgagcccat gcccgagtgc ttcgacgagg tgcaaggcga cctcaaagcc gccgtgaccg
     2581 gagccgagat cgtcttcgtc accctcccgt tgaccgacgc cactcgcggc ct
//
LOCUS       DAOPHV010000384         2628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_54923_length_2628_cov_3.392538, whole genome shotgun sequence.
ACCESSION   DAOPHV010000384 DAOPHV010000000
VERSION     DAOPHV010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54923_length_2628_cov_3.392538"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="PLQ29_09755"
     CDS             complement(<1..1124)
                     /locus_tag="PLQ29_09755"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPG86972.1"
                     /translation="MKKKILICDDEANVRTYLGDLLALEGFAVVEADSGSSAVAAVSR
                     ERPELCVLDLMLPDCNGVELLPRLKAADPMLAVIVITALGTVDNAVLSMKAGAYDFIT
                     KPFDMDTILMSVRRALDFVSVSRENTVLWNLHKNRVYYEEFIGECPAVAQIKALIPKL
                     AFADVPILITGETGTGKNVLAKQIHFTLSGAQAPLVYANCSSIQATLFESELFGHEKG
                     SFTGAAAQKKGRVELADGGTLLLDEITEIPYEIQAKLLDFLQERSFYRVGGVAPISVD
                     IRVIALTNKNLQDEIEAGRFRKDLFYRLNVIHFNVPPLRERGDDILLVCEYLLGLLRR
                     KYNNPSIRLSASAEALVRGYSWPGNVRELKNALERAFICA"
     gene            complement(1124..>2628)
                     /locus_tag="PLQ29_09760"
     CDS             complement(1124..>2628)
                     /locus_tag="PLQ29_09760"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG86973.1"
                     /translation="LDAVLTYGPSEQFQYTVGRPIFEGHKGYEVMATGEPMVQTGSLV
                     RGDILNCMWPVKRDGRVIGYIWSNETLDMIDDQLDPILDRVVLIQVLIFILIYLSLTV
                     TMKGLLDKITSIKRGIEVLFHMPNYHIPQVSGELSTIVSTINDLVDTNNLVKCYNKNI
                     LEGVMNGVLAVSCSGAVTRANKAFLELFPELGSGFIDAHYRDALGPVLAPVIERMEKA
                     LEGRGSLSGVELGYGDRVIEAYGSAMFDETGSRIGAVYVFRDVTTMRRYEKEIKEKEH
                     AVLLSEMALAVVHEVKNPMTSVKGFAQLLGREGVGEEKRREYLRLIDEELNRVNRLLN
                     EMLAYGGESRLEPRPCDVAELVRDAARRNDWSPYGVRPTVLVDGDGDYLAPVDGFKLG
                     QVLDNVLKNACEAVSAKGSGRVVAQVAQAGDAVTVRVVDSGVGIAPDALRRIGSPLFS
                     TKKDGNGFGLAISRKIVEAHGGSLFVDSREGLYTKVSVFLPKCLPPDGGA"
BASE COUNT          436 a          861 c          837 g          494 t
ORIGIN      
        1 tcggcgcaga taaacgccct ctcgagcgcg ttcttgagct cgcgtacgtt acccggccac
       61 gagtagcccc ggacgagggc ctcggccgac gccgatagcc gtatcgatgg attgttgtac
      121 ttgcgcctga gcagcccgag caggtactcg cagacgagga ggatgtcgtc gccgcgctcg
      181 cggagcggcg gcacgttgaa gtggatcacg ttgagccggt agaacaggtc cttcctgaag
      241 cggccggcct cgatctcgtc ctgcaggttc ttgttggtca gcgctatcac gcggatatcg
      301 acggagatcg gcgcgacgcc gccgacccgg tagaagctac gctcctgcag gaagtcgagc
      361 agcttggcct ggatctcgta gggtatctcg gtgatctcgt cgaggagcag ggtaccgcca
      421 tcggccagct cgacgcgtcc cttcttctgg gccgcggcgc cggtgaagct tcccttctcg
      481 tggccgaaca gctccgactc gaacagcgtg gcctggatgc tcgagcagtt ggcatagacg
      541 agcggcgcct gggcgcccga tagcgtgaag tggatctgtt tggccagcac gttcttgccg
      601 gtgcccgtct cgccggtgat caggatcggc acgtcggcga aggccagctt gggtatcaat
      661 gccttgatct gggccacggc cgggcactcg cctatgaact cctcgtagta gacgcggttc
      721 ttatgcaggt tccacaggac cgtgttctcc ctgctgacgg acacgaagtc gagcgcgcgc
      781 ctgacggaca tcaggatcgt gtccatgtcg aatggcttcg taatgaagtc gtaggcgccg
      841 gccttcatcg atagcacggc gttgtcgacg gtgccgagcg ccgtgatcac gatgaccgcc
      901 agcatcgggt ccgcggcctt gagcctgggc aggagctcga cgccgttgca gtccggcagc
      961 atgaggtcga gtacgcatag ctccggccgc tccctggaca cggctgccac ggccgaagag
     1021 ccggaatccg cctcgacgac ggcgaagccc tcgagagcca ggaggtcgcc gaggtaggtc
     1081 ctgacgttcg cctcgtcgtc gcatatgagt atcttcttct tcatcaagcg cctccatcgg
     1141 gcggcaggca ctttggcagg aagacgctca ccttcgtata gagcccctcc ctcgaatcga
     1201 cgaagaggct gccgccgtgc gcctcgacga tcttcctcga aatagccagc ccgaagccgt
     1261 tgccgtcctt cttggtggag aacagcgggc tgcctatcct gcgcagcgcg tcgggcgcta
     1321 tgccgacgcc tgagtccacg acgcggaccg tcaccgcgtc gcccgcctgg gcgacctggg
     1381 cgacgacgcg gcccgagccc ttggcggaca ccgcctcgca ggcgttcttg agcacgttgt
     1441 cgagtacctg cccgagcttg aacccgtcga cgggcgccag gtagtcgccg tcgccatcga
     1501 ccagcacggt cggccggacg ccgtaggggc tccaatcgtt gcggcgcgcc gcgtcgcgga
     1561 ccagctcggc cacatcgcag ggccgcggct ccaggcggct ctcgcccccg tacgcgagca
     1621 tctcgttcag gagccggttc acccggttca gctcctcgtc gattaggcgc aggtactcac
     1681 gccgtttctc ctccccgacg ccctcccgtc ccaggagctg ggcgaagcct ttgaccgagg
     1741 tcatcgggtt cttgacctcg tgtacgaccg cgagcgccat ctcgctcagg agcacggcgt
     1801 gttccttctc cttgatctcc ttctcgtagc gccgcatcgt cgtcacgtcg cggaacacgt
     1861 acacggcgcc tatgcgcgaa ccggtctcgt cgaacatggc gctgccgtag gcctcgatga
     1921 cgcggtcgcc gtagcccagc tcgacgccgg acagggagcc gcgcccctcg agggccttct
     1981 ccatgcgctc gatcaccggc gccaggacgg gcccgagcgc gtcgcggtag tgcgcgtcga
     2041 tgaagcccga cccgagctcg gggaacagct cgaggaaggc cttgttggcc ctcgtcaccg
     2101 cgcccgagca cgagacggcg agcacgccgt tcatcacgcc ctcgagtatg ttcttgttgt
     2161 agcacttgac caggttgttg gtgtcgacga gatcgttgat cgtgctaacg atcgtcgaaa
     2221 gctcgccgga gacctggggt atatggtagt tgggcatgtg gaacaggacc tcgatgcctc
     2281 gcttgatcga ggtgatcttg tccaagaggc ccttcatcgt caccgtcagg ctcaggtaga
     2341 tcaggatgaa aatgaggacc tggatcagca cgacgcggtc caggatgggg tcgagctggt
     2401 cgtctatcat gtcgagggtc tcgttcgacc agatgtagcc gataacccgg ccgtcgcgct
     2461 tgaccggcca catgcagttg aggatatcac cacgtaccag cgatccggtc tggaccatcg
     2521 gctcgcccgt cgccatcacc tcgtagccct tgtgcccctc gaaaataggc cgcccgaccg
     2581 tgtactggaa ctgctcgctc ggcccgtagg tgagcaccgc gtccaggt
//
LOCUS       DAOPHV010000385         2623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_55069_length_2623_cov_2.794003, whole genome shotgun sequence.
ACCESSION   DAOPHV010000385 DAOPHV010000000
VERSION     DAOPHV010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55069_length_2623_cov_2.794003"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PLQ29_09765"
     CDS             complement(<1..873)
                     /locus_tag="PLQ29_09765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HPG86974.1"
                     /translation="MTAPAEPVARGIIARGAVARILPRSAVDGPGNRAVVFMQGCDFD
                     CLYCHNPETRALCVHCGECAPACPSGALTARPAAGPERPFGLEVAWDATRCVDCGACV
                     AACRRDSSPKAVTMSAAEVLASLGRQRAFLSGLTVSGGECALQPAFLESLLVASRAAG
                     LPGLVDTNGSTDYRELPGVVEAAEGFMLDVKAWDEDEHRALTGASNRAAIANLRFLAA
                     AGKLYEVRTVVVPGRFDAELTVREVSRALVDSGSAARYKLIRFRPQGVRACWRDLPLP
                     DDGMMAALAGLARAE"
     gene            complement(870..2375)
                     /locus_tag="PLQ29_09770"
     CDS             complement(870..2375)
                     /locus_tag="PLQ29_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HPG86975.1"
                     /translation="MSASLSVITDRTLTYQQKVAALAKVGEETARPLSIDPETQALRD
                     AGVICDLFEGSAPFRPRYVVPDYERFMRRGSAFLGLEPPRDLWEATTALLVLYRHVPS
                     ITTFPVYLGDLDALLEPFVRDEAEARKAIGLFLLSIDRTLNDSFCHADIGPDATTAGR
                     LILELSREQKNAVPNISLKYDPDRTPDDFAILAAATALEVAKPSFANHRLFASEFEAM
                     GLGDYAIASCYNGLAKGGGSCTLVRMNLAALSPRSKGLDDFMDRVLPDAVERQLSYMD
                     ERVRFLVDESGFFESSFLVAEGLVRKERFSAMFGLVGLAECANDLASRGTAGAGRFGH
                     DAGTDALALAIVERIASIVGSHRVERLEANGGRYLLHAQVGIDSDRGVSPGCRVPIGE
                     EPELVEHILQSAPFHRYFPSGIGDIFAFEPTAKANPRQLLDIVRGGFSHGLRYLSAYA
                     ADADVIRITGYLVKRSEIARLNSGGQSLQDTTALGRNAVNNLGVLERRLRP"
BASE COUNT          401 a          895 c          960 g          367 t
ORIGIN      
        1 ctcggctcgg gccaggcccg ccagggccgc catcatcccg tcgtcgggca ggggcaggtc
       61 gcgccagcac gcgcgcacgc cctggggccg gaagcgtatc agcttgtagc gcgccgccga
      121 gccgctatcg accagcgccc gggatacctc gcgtacggtc agctccgcgt cgaaacgccc
      181 gggaacgacg acggtacgga cctcgtacag cttaccggcg gcggcaagga aacgcaggtt
      241 ggctatcgcc gcccggttcg aggcgccggt cagggcgcgg tgctcgtcct cgtcccaggc
      301 cttcacgtcg agcatgaagc cctccgccgc ctcgacgacg cccggcagct cccggtaatc
      361 ggtcgagccg ttcgtgtcga cgaggccggg cagcccggcg gcccgcgacg cgacgaggag
      421 cgactcgagg aaggccggct gcagagcgca ctctccgcct gagactgtga ggcccgacag
      481 gaaggcgcgc tgccgcccga gcgacgccag gacctccgcg gcactcatcg tcacggcctt
      541 cggcgacgag tcgcgccggc aggcggcgac gcaggcgccg cagtcgacgc agcgggtcgc
      601 gtcccacgcc acctcgagcc cgaacggccg ctccggaccg gcggcgggcc tcgcggtcag
      661 cgcgccggac ggacaggcgg gcgcgcactc gccgcagtgg acgcataggg cgcgcgtctc
      721 ggggttgtgg cagtacaggc agtcgaagtc gcagccctgc atgaacacga cggcccggtt
      781 gcccggcccg tcgaccgcgc tgcgcggcag tatgcgcgcg accgcgccgc gcgcgatgat
      841 gccgcgcgcg acgggctcgg ccggagccgt cacgggcgga gccggcgctc gagcacgccg
      901 aggttgttga ccgcgttgcg gccgagcgcg gtcgtgtcct ggagcgattg gccgcctgag
      961 ttcagcctgg cgatctccga gcgcttgacg aggtagccgg tgatcctgat gacgtcggcg
     1021 tcggcggcgt aggcgctcag gtagcgcagc ccgtgcgaga agccgccgcg cacgatgtcc
     1081 aggagctggc gcgggttggc cttggcggtc ggctcgaagg cgaaaatgtc gccgatgccg
     1141 ctcgggaagt agcgatggaa gggcgccgac tggaggatgt gctccaccag ctccggctcc
     1201 tcgcctatgg gcacgcggca gcccggactg acgccccggt cgctgtcgat gcccacctgc
     1261 gcgtgcagga ggtagcggcc gccgttggcc tcgaggcgct cgacgcggtg cgagccgacg
     1321 atggacgcga tgcgctcgac gatcgccagc gcgagcgcgt cggtaccggc gtcatggccg
     1381 aagcgcccgg cacccgctgt gcctcgggag gccaggtcgt tggcgcactc ggccagcccg
     1441 acgaggccga acatcgcgct gaagcgctcc ttgcgcacga ggccctcggc gacgaggaag
     1501 cttgactcga agaagcccga ctcgtcgacc aggaagcgca cccgctcgtc catgtacgag
     1561 agctgccgct cgacggcgtc gggcaggacc cggtccatga agtcgtccag gcccttcgag
     1621 cgcggcgaca gggccgcgag gttcatcctg acgagcgtgc acgagccgcc gcccttggca
     1681 aggccgttgt agcacgaggc tatcgcgtag tcgcccaggc ccatcgcctc gaactcggag
     1741 gcgaacaggc ggtggttcgc gaagctcggc ttggccacct cgagcgccgt cgccgcggcg
     1801 aggatcgcga agtcgtccgg cgtgcgatcg gggtcgtact tgaggctgat attcggcacc
     1861 gcgttcttct gctcgcgaga cagctcgagt atcagccgac cggccgtggt ggcgtcgggc
     1921 ccgatgtcag cgtggcagaa cgagtcgttc agcgtacggt ctatcgacag gaggaacagc
     1981 ccgatggcct tgcgggcctc ggcctcgtca cgcacgaagg gctcgagcag ggcgtcgagg
     2041 tcgccgaggt agacggggaa ggtggtgatg ctcggcacgt ggcggtacag gacgaggagg
     2101 gccgtcgtcg cctcccagag gtcgcggggc ggctcgagcc cgaggaaggc cgagccccgc
     2161 cgcatgaagc gctcgtagtc cggcaccacg tagcgcggcc ggaacggcgc ggagccctcg
     2221 aacaggtcgc agatgacgcc cgcgtcgcgc agggcctggg tctcggggtc gatggagagg
     2281 gggcgggcgg tctcctcgcc gaccttggcg agtgccgcga ccttctgctg ataggtaagg
     2341 gtacgatcgg taatgaccga caaagacgcg ctcatggcta tactcccgta tcgagtcgtt
     2401 gaagctctgg aagtttaacc gcgccggttt tgacggcccg cgtacggggc cgctccctac
     2461 gatgcgctcc gcgacgaagg acgcgcgtgc taaccggggt tctttatcgc gtcgacgcgc
     2521 gatgttggcc cctagtatac ccctccgccg ccgacgaaac aagtccgcga tcgccgactg
     2581 tcgcggcgat cgccggccgt cgttcgaccc gactcgcttc cgc
//
LOCUS       DAOPHV010000386         2616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_55264_length_2616_cov_4.346740, whole genome shotgun sequence.
ACCESSION   DAOPHV010000386 DAOPHV010000000
VERSION     DAOPHV010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55264_length_2616_cov_4.346740"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLQ29_09775"
     CDS             <1..204
                     /locus_tag="PLQ29_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HPG86976.1"
                     /translation="MKPQEADLFNHHACLCHSLLEDTSLFLAIGTPLGWITLPRIVAA
                     LLVVWLERLRRGRFRRSFRAGTA"
     gene            complement(201..1142)
                     /locus_tag="PLQ29_09780"
     CDS             complement(201..1142)
                     /locus_tag="PLQ29_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPG86977.1"
                     /translation="MIADVSILVALGAGLLSFLSPCVLPLIPGYLSFISGAGIERIRD
                     GSERSGVFWRTLFFVTGFSAVFIALGLAFSGGGMLTAGRANRVVTVAAGSLIAALGLN
                     MIFGFLRFLNLEARVKAPAKPRSAAGAFVVGMAFGAGWTPCVGPILASILVMAARSGG
                     AGRAVMLLTAYSAGLALPFIAAGLFFERLSPVWAWFKRHGRGVQIVSGLLLLAIGVSM
                     AIGRLGSIGAISARAGIGLKAALATDPAAVRLWSAAVVAALAAILVVAPLARRRPFAK
                     PWRIVALALLAVLLTLEAAGAVSMARLIADWLLFQGA"
     gene            complement(1139..1771)
                     /locus_tag="PLQ29_09785"
     CDS             complement(1139..1771)
                     /locus_tag="PLQ29_09785"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPG86978.1"
                     /translation="MISMPRTVIAASLALLSLASCSGPKDGEAPVSTMASAAPSSAET
                     PSILAADAAPWYAEPMEALGFFVFPRPEPMPPFEVAPLAGGRAGLGDAAGKVVLLNFW
                     ATWCPPCRSEMPSIQRLHDAMAGRPFAVMAVSVAESAATVRAFLKTDPYTFPIYLDEG
                     GRASGPFVGRGIPTTFVLDKQGRAIAGVIGSRSYDDPEVLALFKALAERL"
     gene            1998..2609
                     /locus_tag="PLQ29_09790"
     CDS             1998..2609
                     /locus_tag="PLQ29_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPG86979.1"
                     /translation="MVSQIYGRLDDDDEEEDKTQRGPDVLAERLLETRTVLLSGQVNK
                     ELAERVVRQLLLLEDKGPEPIKIMIDSPGGDVDAGYAIFDMARFVKPEVWMIGMGLVA
                     SAGALILLAAPKKRRIAFPNSHYLIHQPLSGLRGVATEIEIHAREIEKTRDRINKLIA
                     AETGQPLERVVKDTDRDYWMGAEDAMSYGLVSRIVSTRDELGL"
BASE COUNT          507 a          867 c          873 g          369 t
ORIGIN      
        1 atgaagccgc aggaggccga cctgttcaac caccacgcct gcctatgcca cagcctgctc
       61 gaggacacgt cgctgttcct ggcgatcggc acgccgctag gctggattac cctgcccagg
      121 atcgtcgccg ccctcctggt cgtctggctc gagcgcctca ggcgcgggcg attcaggcgc
      181 tcgttcaggg ccggtacggc ctaggctccc tggaacaaca gccaatcggc tatcagtctc
      241 gccatcgata cggcgcccgc ggcctcgagc gtcagtagaa cggcgaggag ggccagcgcg
      301 acgatacgcc atggcttggc gaagggccgg cgccgcgcca gcggcgcgac gaccaggata
      361 gccgcgagcg cggcgacgac ggccgcgctc catagcctga cggccgccgg atcggtggcc
      421 agggcggcct tcaagcctat gccggccctg gcgctgatcg cgcctatcga gccgagccgg
      481 cctatggcca tcgatacgcc gatagcgagc agtaggaggc ccgagacgat ctggacgccg
      541 cgtccatggc gcttgaacca ggcccagacc ggtgacaagc gctcgaagaa caggcccgcg
      601 gcgatgaacg gcagggccaa gccggccgaa taggccgtca ggagcatgac cgcccggccg
      661 gccccaccgg accgtgcggc cattacgagt atcgaggcca ggataggccc cacgcagggc
      721 gtccagccgg ccccgaaggc catgccgacg acgaaggcac cggcggcgct cctcggcttg
      781 gccggggcct tgaccctggc ttcgaggttc aagaagcgta ggaagccgaa gatcatgttc
      841 agcccgagcg ccgcgatgag cgaaccggcc gcgaccgtca ccacgcggtt ggcgcgcccc
      901 gcggtcagca tgccgccgcc cgagaacgct agccccagcg ctatgaacac ggccgagaag
      961 ccggtcacga aaaacaacgt cctccagaag acgccggagc gctcagaacc gtcgcgtatg
     1021 cgctcgatgc cggcaccgct gatgaatgac agatagccgg ggatcaacgg cagcacgcat
     1081 ggcgatagga acgacaggag ccccgcgccc agggcgacga gtatcgatac gtcagcgatc
     1141 acagacgctc cgcgagcgcc ttgaacagcg ccaacacctc ggggtcgtcg taggaccggg
     1201 agcctatgac gccggctatc gcccggccct gcttgtcgag tacgaaggtg gtgggtatgc
     1261 cgcggcccac gaaggggccg gaggcccggc ctccctcgtc gaggtagatc gggaaggtat
     1321 agggatccgt cttgaggaag gcacgcacag tcgcggccga ttcggctacg ctaaccgcca
     1381 tcaccgcgaa gggccggccg gccatggcgt cgtggaggcg ctgaatggac ggcatctcgc
     1441 tacggcaggg cgggcaccag gtggcccaga agttgagcag gacgaccttg ccggccgcgt
     1501 cgcccagtcc cgccctgccg ccggccaggg gcgcgacctc gaagggcggc atcggttccg
     1561 gcctcgggaa cacgaagaag ccgagcgcct ccataggctc ggcgtaccac ggcgcggcgt
     1621 ccgccgccaa tatcgagggc gtttcggcgg aggagggcgc cgcggaggcc atcgtcgata
     1681 cgggggcttc tccgtccttc ggccccgagc acgaggcgag gctaagcagg gcgagcgaag
     1741 cggctatgac tgtccggggc atcgatatca tcgcgtctcc tattggcccg ctatacgacg
     1801 ggttcgcaac cctgatgcgt caaggtagcg catggcggat tgggaattat ataggcaatt
     1861 tactatattt caagcattcc agcaagcgcc gcctcggacc gccgcatagg cgaatcggca
     1921 cttttttatt atactagctt ttggttcgcc tacgcgcgag cctttaccaa caccgaattc
     1981 gaggagacga catgagcatg gtcagtcaga tatatgggcg cctggacgac gacgacgagg
     2041 aggaggacaa gactcagcgg ggcccggacg tcctcgccga gcgcctactc gagacgcgta
     2101 ccgtcctgct gtccggacag gtgaacaagg aactggccga gcgggtcgtc cgccagctgc
     2161 tattgctcga ggacaagggc cccgagccga tcaagatcat gatagactcc cccggcggag
     2221 acgtcgatgc cggctacgcc atcttcgaca tggcccgctt cgtcaaaccg gaggtctgga
     2281 tgataggcat gggcctcgtc gcgagcgccg gcgccctcat cctgctagcg gcccccaaga
     2341 agcgccgcat cgcgttcccg aactcgcact acctgatcca ccagccgctg tcgggactgc
     2401 gcggcgtggc caccgagata gagatccacg cccgcgagat cgagaagacc cgcgaccgca
     2461 tcaacaagct gatagccgcg gagaccggcc agccgctcga gcgcgtcgtc aaggacaccg
     2521 accgggacta ctggatggga gccgaggacg ccatgtcgta cgggctggtc tccaggatcg
     2581 tgtcgacgag ggacgaactc ggcctctagc cggggt
//
LOCUS       DAOPHV010000387         2611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_55409_length_2611_cov_4.226135, whole genome shotgun sequence.
ACCESSION   DAOPHV010000387 DAOPHV010000000
VERSION     DAOPHV010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55409_length_2611_cov_4.226135"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1193
                     /locus_tag="PLQ29_09795"
     CDS             108..1193
                     /locus_tag="PLQ29_09795"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HPG86980.1"
                     /translation="MKVAILYGGKSGEHEVSLRSAASVVRHLDGSHKVQLIGVAKDGA
                     WYLQPDSVLASCLEGDQPLELRADGPTVLAVPGRGLRVYGARGSADLRLDAVFPVLHG
                     TFGEDGTVQGLLECAELPYVGADVAGSAIAMDKELAKSLWLQAGLPVVPSITARPADA
                     DKVDELAERIESAFGWPAFVKPARCGSSVGASKISGPADLQGAVRSALLYDTKAVIEP
                     FIQAREIECAVLGNDEPRAFPPGEIVPSHEFYDYDAKYIDPDGAALVIPAAIDEATAA
                     RVMDIAVAAYKAASLSGMARVDFFIDKKDGSVRLNEANSIPGFTSISMYPMMCEAGGL
                     SYSALLDELLELAVDKKRQRDALRFSR"
     gene            1332..2534
                     /gene="rocD"
                     /locus_tag="PLQ29_09800"
     CDS             1332..2534
                     /gene="rocD"
                     /locus_tag="PLQ29_09800"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HPG86981.1"
                     /translation="MKSGDYIEMDEKYGAHNYHPLPVVISWALGTKVKDPDGKEYYDF
                     LSAYSAVNQGHRHPRIIKALVDQANVCTLTSRAFHNDRMGLFLKKLCEFTGYEMALPM
                     NTGVEAVETAIKAARRWGAEVKGVPNGKQEVICADDNFHGRTLVAISMSNDPDSTVNY
                     GPYVPGFVRVPYGDADAIVKAITPNTVAVIVEPIQGEAGVVVPPAGYLAKIREACDKN
                     KVLFIADEVQTGFCRTGKRFAWQHEGARPDIMCLGKALGGGVMPVSAIVADQRIMKVF
                     TPGTHGSTFGGNPLSSSVAIAALEVLEDEKLDQNAERLGKIFRDEVAKIKCPRMVTVR
                     GKGLLNAVVFEKGFEAWNVCLSLRDAGLLAKQTHGNIIRFAPPLVITEKEMMEAVAII
                     KRVFEKIA"
BASE COUNT          495 a          869 c          802 g          445 t
ORIGIN      
        1 gactacgctt cgggttcgat accctggaac gagatagacg aggccggaaa ggcgctcgag
       61 gaggcgggat ataccctccg gagcggcggc caagataagg aatcacgatg aaagtagcaa
      121 tcctatacgg cggcaaatcc ggcgagcatg aggtttcgct ccgatcggcg gccagcgtcg
      181 tacgtcacct ggacggatcg cataaggtcc agctcatcgg cgtcgccaag gacggcgcct
      241 ggtaccttca gccggacagc gtgctcgctt cctgcctcga gggcgaccag ccgctcgagc
      301 tgcgcgccga cgggccgacc gtgctcgccg tccccggcag gggcctccgc gtctatggcg
      361 cgcggggttc cgccgacctg aggctggacg cggtcttccc ggtgctacac ggcaccttcg
      421 gcgaggacgg caccgtgcag ggactcctcg agtgcgccga gctaccgtac gtcggagccg
      481 acgtggctgg ctcggccata gccatggata aggagctggc caagtcgcta tggctccagg
      541 ccggacttcc ggtcgtgcct tcgatcaccg ctcggccggc cgatgcggac aaggtcgacg
      601 agctggctga gcgcatcgag tcggccttcg gctggcctgc cttcgtgaag ccggcgcgct
      661 gcggctcgag cgtcggcgcg tcgaagataa gcggcccggc cgatctccag ggcgccgtcc
      721 gctcggccct gctctacgat accaaggccg tgatcgagcc gttcatccag gcgcgggaga
      781 tcgagtgcgc cgtactcggc aacgacgagc ccagggcctt ccctcccggc gagatcgtgc
      841 cgtcgcacga gttctacgat tacgacgcca agtatatcga cccggacggc gccgccctcg
      901 tcatcccggc cgcgatagac gaggcgacgg ccgctcgcgt gatggacata gccgtggccg
      961 cctacaaggc ggccagcctc tccggcatgg ccagggtaga cttcttcatc gacaagaagg
     1021 acggctcggt acgcctcaac gaggcgaact ccattcccgg cttcacctcc atctcgatgt
     1081 accccatgat gtgcgaggcc gggggcctgt cctattctgc cttgctcgac gaactcctcg
     1141 agcttgccgt cgacaagaag cgccagcggg acgcgctcag gttctcgcgt tgatatttat
     1201 gcacttcata cgaataatta ttgatattgc atatagttaa aagctcggat acgatgattc
     1261 gtctggaagg cgtcgtaagc gcgtatcgta acgcctcggc cgacgcgagg ctctttcgta
     1321 ggaggtatcg aatgaagagc ggcgattaca tcgaaatgga cgagaagtac ggggctcata
     1381 actaccaccc actacccgtg gtcatctcat gggccctcgg taccaaggtc aaggacccgg
     1441 atggcaagga atactacgat ttcctctcgg cctactccgc ggtcaaccag ggccaccgcc
     1501 acccgaggat catcaaggcc ctcgtcgatc aggccaatgt ctgcacgctg acgagccgag
     1561 cctttcacaa cgacaggatg ggcctgttcc tcaagaagct ctgcgaattc accggctacg
     1621 agatggcctt gccgatgaac accggcgtcg aggcggtcga gaccgccatc aaggccgccc
     1681 gccgctgggg cgccgaggtc aagggcgtgc cgaacggcaa gcaggaagtg atctgcgccg
     1741 acgacaactt ccacggccgt accctcgtcg ccatctccat gtcgaacgac cccgattcta
     1801 ccgtcaacta cggcccctac gtacccgggt tcgtgagggt gccctacggc gatgccgacg
     1861 ctatcgtcaa ggccatcacg ccgaacaccg tggccgtcat cgtcgagccc atccagggag
     1921 aggccggcgt cgtcgtgccg cccgccggct acctggccaa gatccgcgag gcctgcgaca
     1981 agaataaggt cctgttcata gccgacgagg tccagacagg cttctgccgg accggcaagc
     2041 gcttcgcctg gcagcacgag ggcgctcgtc ccgacatcat gtgcctcggc aaggccctcg
     2101 gcggcggcgt catgcctgta tccgccatcg tcgccgacca gaggatcatg aaggtcttca
     2161 ctcccggcac ccacggctcc accttcggcg gcaacccgct gtcgagctcg gtcgccattg
     2221 cggccctcga ggtgctcgag gacgagaagc ttgaccagaa cgccgagcgc ctcggtaaga
     2281 tcttcaggga cgaagtcgcc aagatcaagt gccccaggat ggtcaccgtc cgcggcaagg
     2341 gcctcctcaa cgccgtcgtg ttcgagaagg gcttcgaggc ctggaacgtc tgcctctctc
     2401 tacgcgacgc cggcctcctg gccaagcaga cccacggtaa catcatccgc ttcgccccgc
     2461 ccctcgtcat caccgagaag gagatgatgg aggccgtcgc gatcatcaag cgcgtcttcg
     2521 agaagatcgc ctgatagcgc gacgagtcgc ccaagaacgc tagccgcgcg ctatcgcgac
     2581 tacgccccgg cttaggccgg ggcgcttttt t
//
LOCUS       DAOPHV010000388         2607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_55520_length_2607_cov_3.596787, whole genome shotgun sequence.
ACCESSION   DAOPHV010000388 DAOPHV010000000
VERSION     DAOPHV010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55520_length_2607_cov_3.596787"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PLQ29_09805"
     CDS             <1..421
                     /locus_tag="PLQ29_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG86982.1"
                     /translation="DDIRRAAEAVRAAGFIERLEGGYGATLGEGAATLSSGQRQLLSF
                     ARVVAHDPAIVVLDEATSSVDTETEQLLQEGLAAMMRGRTTVAIAHRLSTIRHADRII
                     VLADGRLAEEGDHDSLIAKGGIYYNLYRLQYQGEASA"
     gene            421..1785
                     /locus_tag="PLQ29_09810"
     CDS             421..1785
                     /locus_tag="PLQ29_09810"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPG86983.1"
                     /translation="MEKSIRFNILALVGPETAGVVRRAVDEVGGCDLRVDAGESALVA
                     VESGDQVDLVLLDPDAVSMATADGIAVLKAACPSCEIALLLGDHDITMDVELMRLGAY
                     DSLPNPPGVDSLALLIRGLMEKNETERENRAMVEAMGQGRSQTIISHSPEMEKVLSMA
                     ARAAKSDSTVLITGESGTGKELVARAIHLAGPRKHKAFVPVNIAALPENLIESELFGH
                     VKGAFTGAMADRTGRFALADGGTLFIDEIGEVPPGIQVKLLRVLQFGSYERVGENAPT
                     VSDVRIIAATNRDLEAEVRAGRFRADLYYRVNVVPVSLPALRDHRADIPYLVEYFIKK
                     YATKNRKPVRSITGEAMARIMRYPFPGNVRELENVLERGVVLCRGEYLTETDIFLPEE
                     PNGSGDDTPMPPGGYDEVMSAFEREYLGKVLERNAHNKSAAARELGINERRLRYRLKV
                     LGIE"
     gene            complement(1800..2351)
                     /locus_tag="PLQ29_09815"
     CDS             complement(1800..2351)
                     /locus_tag="PLQ29_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86984.1"
                     /translation="MRRATLFAAIVALMAAAGSAFAQATPSGEAAVPRSFRGIELGMS
                     MDEVKAALAADGLFAYRGDPDVSLLPRPDESLIEVAGVSFVRRAFFQFYGGRLFVMIM
                     ALNEREMDHYSVFTTLAGKYGKPDSLSPSESVWEDGATRLSVERPLAVKYIDLAVFNE
                     LKAAGSAVRSYEEILRSEFLGGF"
     gene            complement(2365..>2607)
                     /locus_tag="PLQ29_09820"
     CDS             complement(2365..>2607)
                     /locus_tag="PLQ29_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPG86985.1"
                     /translation="PVFGEMSMFSDEPRSATVGAASDCVLFEVTRRSFEAICARDPRL
                     GLELTRRIAVMLASRVRKGNDDVLKLSTALSIALSR"
BASE COUNT          471 a          851 c          887 g          398 t
ORIGIN      
        1 ggacgatatc cggagagcgg ccgaggccgt gcgggccgcc ggcttcatcg agcgcttgga
       61 aggcggctac ggcgccacgc tcggcgaggg ggccgcgacg ttgtcctccg gtcagcggca
      121 gctcctgtcg ttcgcgcgcg tcgtcgcgca cgacccggcc atcgtcgtgc tggacgaggc
      181 gaccagctcg gtggataccg agaccgagca gctcctgcag gagggcctgg cggcgatgat
      241 gcgcggccgg acgaccgtcg ccatagccca ccgcctgtcg accatacgcc acgccgaccg
      301 catcatagtg ctcgcggacg gcaggctggc cgaggaaggc gatcacgata gtcttatcgc
      361 caaaggcggc atatactaca acctgtaccg cctgcaatac caaggggagg cttccgccta
      421 atggaaaaaa gtatccgatt caatatcctg gccctggtcg gtccagagac cgcgggcgtc
      481 gtgaggcgag cggtcgacga ggtggggggc tgcgacctgc gcgtcgacgc cggcgaatcg
      541 gccctcgtcg ccgtcgagtc cggcgatcag gtggacctcg tgttgctcga cccggacgcc
      601 gtctcgatgg ccacggccga cggcatcgcc gtgctcaagg cggcctgccc gagttgcgag
      661 atcgccttgc tactcggcga ccacgacatt accatggacg tggagctgat gcggctcggc
      721 gcctacgatt cgttgccgaa cccgccgggc gtcgattccc tggccctttt gatacgcggc
      781 ctcatggaga agaacgagac cgagcgcgag aaccgcgcca tggtcgaggc gatggggcaa
      841 ggccgttccc agacgatcat ctcccatagc cccgagatgg agaaggtgct gtcgatggcc
      901 gccagggccg ccaagagcga ctcgacggta ctcataaccg gggagagtgg caccggcaag
      961 gaactggtgg ccagggccat ccatctggca gggccgcgca agcacaaggc cttcgttccg
     1021 gtgaacatag cggccctgcc ggagaacctg atcgagagcg agctgttcgg tcacgtcaag
     1081 ggcgccttca ccggcgcgat ggccgatagg acggggcgat tcgcgctggc cgacggcggc
     1141 acgctgttca tagacgagat aggcgaggtt ccgccgggca tccaggtcaa gctcctgcgc
     1201 gtgctgcagt tcggctcgta cgagcgggtc ggcgagaacg cgccgaccgt ctccgacgtg
     1261 cgcatcatcg ccgcgaccaa ccgcgacctg gaggccgagg tccgcgccgg cagattccgc
     1321 gccgacctgt actatcgcgt caacgtggta ccggtatcgc tgccagccct gcgcgaccac
     1381 cgcgccgaca taccctatct cgtcgagtac ttcatcaaga agtacgcgac caagaaccgc
     1441 aagccggtca ggagcatcac cggcgaggcg atggccagga tcatgcgcta tcccttcccg
     1501 ggcaatgtgc gcgagctgga gaacgtgctc gagcgtggcg tcgtgctatg ccgcggcgag
     1561 tatttgaccg agacggatat tttcctaccc gaggagccga atgggagtgg cgacgatacg
     1621 ccgatgccgc cggggggcta cgacgaggtg atgagcgcct tcgagcgcga gtacctgggc
     1681 aaggtgctcg agcggaacgc ccataacaag agcgccgccg cccgcgagct ggggatcaac
     1741 gagcggcgcc tccgctatcg gctcaaggtg ctcgggatcg agtaggggct cggcgagagc
     1801 tagaagccgc cgaggaactc cgagcgcagg atctcctcgt aggagcgtac ggcgctcccg
     1861 gcggccttga gctcgttgaa gaccgcgagg tcgatgtact tgacggcgag cggccgctcg
     1921 accgacagcc tggtcgctcc gtcctcccag acgctctcgc tcggggacag cgagtcgggc
     1981 ttgccatact tacccgccag cgtggtgaag accgagtagt ggtccatctc gcgctcgttc
     2041 agggccatga tcatcacgaa cagccgacct ccgtagaact ggaagaaggc gcgcctgacg
     2101 aaggatacgc cggcgacctc gatcaggctc tcgtccggcc tcggaaggag gctcacgtcc
     2161 gggtcgccgc ggtacgcgaa caggccgtcg gcggccagcg cggccttgac ctcgtccatg
     2221 ctcataccaa gctcgatgcc cctgaaggaa cgcggcacgg ccgcctcgcc cgacggagtg
     2281 gcctgggcga aggccgagcc tgcggccgcc atcagggcta ctatcgctgc gaatagggtg
     2341 gctcgtctca tcgcggactc ctcgttacct ggatagcgct atcgagaggg ccgtcgacag
     2401 cttgagcacg tcgtcgttac ccttgcggac ccgcgaagcg agcatgacgg cgatgcggcg
     2461 ggtaagctcc agcccgagcc gcgggtcgcg agcgcagatc gcctcgaacg agcgcctggt
     2521 cacctcgaag agcacgcagt cgctggccgc gccgaccgtc gcgctgcgcg gctcgtcgct
     2581 gaacatcgac atctcgccga agaccgg
//
LOCUS       DAOPHV010000389         2600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_55729_length_2600_cov_3.260511, whole genome shotgun sequence.
ACCESSION   DAOPHV010000389 DAOPHV010000000
VERSION     DAOPHV010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55729_length_2600_cov_3.260511"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2003)
                     /locus_tag="PLQ29_09825"
     CDS             complement(<1..2003)
                     /locus_tag="PLQ29_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPG86986.1"
                     /translation="MPNVTAELLAPAGNPEALDAAIGEGADAVYLGLKNFNARMRTNN
                     FAYSQFEAAVESLHKQGRRLYVTVNTVFEQREADRMYQFLQYLERVGPDGIIVQDLGV
                     VKMASEHFPGLKLHASTQMNVGSAAGANFLSRAGFKRVVLSRELSLEELKGVRAETNV
                     ELETFVHGALCVSASGVCLFSSYLGGRSANRGACTQACRRIYADESRDGYFFSPDDLQ
                     LVERVPELVEAGVESLKIEGRMKSAEYVGQVVAAYRYMLDNWRFDRERAATKAVAMLQ
                     GDFARRKTTFWFDGTAAPDFIRPDQAGGTGIPLGKVKVVRVFDEKRWMLVRGFEGIAE
                     GDSIRVHAHGDLNRTTARVRGVVDKPEGLFLQVDGEFGPEDEVYLVQTKSMVKRYKPV
                     LPASLAKYHRFPSFDKAPRPEFPAIPKERLAVLPDGLYTMVSRVQDLHTILTDRPEKA
                     VLRLSRKNAELLRRHEKETPFKRDSLVLWLEPFFPQADAAWLAEELDYWIGKGQRLFV
                     ANNLGHLAMLKGREVTVIAGPWLYAFNAWAAAYLLGNGADFIMPPLEISKQAFGLLAE
                     TVPGKSLFPVVFAYPQLFTIRADLGERFDLRFFRDREGDEYELVSSPDGSVVIPLKPF
                     SLVDRIPFIRKDGATKFLLDFSFIELRKQVYKRVVAAARDGLVL"
     gene            2176..2337
                     /locus_tag="PLQ29_09830"
     CDS             2176..2337
                     /locus_tag="PLQ29_09830"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HPG86987.1"
                     /translation="MREYWIVNPETFEVFVYTLKDDAYGLPAVADLRSPIPVSIFPGL
                     SLRVRPEDL"
BASE COUNT          421 a          880 c          901 g          398 t
ORIGIN      
        1 agcacgagcc cgtcgcgggc cgccgcgacg acgcgcttgt acacctgctt gcgcagttcg
       61 atgaagctga agtccagcag gaacttcgtc gctccgtcct tgcggatgaa ggggatacgg
      121 tcgacgaggg agaagggctt gagcggtatg accacggagc cgtcgggcga ggagacgagc
      181 tcgtactcgt cgccctcccg gtcgcggaag aagcgcaggt cgaagcgctc gccgaggtcc
      241 gcccgtatcg tgaacagctg cgggtacgcg aagaccaccg ggaacaggct cttgcccggc
      301 accgtctcgg cgagtagccc gaaggcctgc ttggatatct cgagcggcgg catgatgaag
      361 tcggccccgt tcccgagcaa gtaggccgcg gcccaggcgt tgaaggcgta cagccagggg
      421 ccggctatca ccgtgacctc gcggcccttg agcatggcca ggtggccgag attgttggcg
      481 acgaagagcc gctgcccctt gcctatccag tagtcgagct cctcggccag ccaggcggcg
      541 tcggcctgag ggaagaacgg ctccagccag aggacgagcg agtcgcgctt gaacggcgtc
      601 tccttctcgt gccgcctgag tagctccgcg ttcttgcgcg acagcctgag cacggccttc
      661 tccggccggt cggtcagtat cgtgtgcagg tcctgcaccc ggctgaccat cgtgtagagg
      721 ccgtcgggca ggacggccag gcgctccttg gggatggccg ggaactcggg ccgcggggcc
      781 ttatcgaagc tcgggaagcg gtggtacttg gccagcgacg cgggcaggac gggcttgtag
      841 cgcttgacca tcgacttggt ctgcacgagg tacacctcgt cctcggggcc gaactcgccg
      901 tcgacctgca ggaagagccc ctcgggcttg tccaccacgc cccggacgcg ggccgtcgtg
      961 cggttcaggt cgccgtgggc gtggacgcgg atcgagtcgc cctcggctat gccctcgaag
     1021 ccgcgcacga gcatccagcg cttctcgtcg aacactcgga ccaccttgac cttgccgagc
     1081 gggatgccgg taccgcccgc ctggtccggg cgtatgaagt ccggcgccgc cgtcccgtcg
     1141 aaccagaacg tcgtcttgcg cctggcgaag tcgccctgca gcatcgcgac cgccttggtc
     1201 gcggcccgct cccggtcgaa gcgccagttg tcgagcatgt agcggtaggc cgcgaccacc
     1261 tggccgacgt actcggcgct cttcatgcgg ccctcgatct tgagcgactc gacgccggcc
     1321 tcgaccagct cgggcacccg ctcgacgagc tgcaggtcgt cggggctgaa gaagtagccg
     1381 tcgcggctct cgtcggcgta gatgcggcgg caggcctggg tgcaggcgcc tcggttggcc
     1441 gagcggccgc ccaggtagct cgagaacagg cagacgcccg acgcgctgac gcagagcgcg
     1501 ccgtggacga aggtctcgag ctcgacgttc gtctccgccc gcacgccctt cagctcctcg
     1561 agcgacagct cgcgcgacag caccacgcgc ttgaagccgg cccgggacag gaagttcgcg
     1621 ccggcggcgc tgccgacgtt catctgcgtc gacgcgtgca gcttgagccc ggggaaatgc
     1681 tccgaggcca tcttcacgac gcccaggtcc tgcacgatga tgccgtcagg ccccaccctc
     1741 tccaggtact gcaggaactg gtacatcctg tcggcctcgc gctgctcgaa gaccgtgttg
     1801 accgtcacgt acagcctgcg gccctgcttg tgcagcgact cgacggccgc ctcgaactgg
     1861 ctataggcga aattattggt gcgcatgcgc gcgttgaaat tcttgagtcc caggtagacg
     1921 gcgtcggcgc cctcgcctat cgcggcgtcc agggcctcgg gattgcccgc cggggcgagc
     1981 agctcggcgg ttacgttcgg catggtatcc tctcggggta tcggcactcg gcccacgggc
     2041 gtcgccatcg acgcccctcg ccctcgcggg gagggcggtc gtacacgcgg gccccgcacg
     2101 ggcgggacgg gccgagccta caagatacta cgattcggcc ccgaatgtat aggagaagac
     2161 gacgagaggc gcggcgtgcg cgagtactgg atagtcaacc cggagacctt cgaggttttc
     2221 gtctatacgc tgaaggacga cgcctacggc ctgccggcgg tcgccgacct gcgaagcccc
     2281 attccggtat caatcttccc cggcctctcg ctcagggtca ggcccgagga cctgtaaggc
     2341 cctcagccga agccgggcac ccggaggagt ccgtctatca cggcgggctc cgaaggagac
     2401 cgccgcgacg gagcgaggcg cggagcgccg gcgcggccgg ataggagcgg agagcccgac
     2461 gccgcacgag cggcggcgcg cccttcatcc ccgaaactat ggacgcgaaa ctgcctcgcg
     2521 ggactccggc agccgcagca ggaagcccag gcagaggcag gcgccggaga cgacggccag
     2581 gcccatgccg gggccgaggg
//
LOCUS       DAOPHV010000390         2590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_56003_length_2590_cov_4.470611, whole genome shotgun sequence.
ACCESSION   DAOPHV010000390 DAOPHV010000000
VERSION     DAOPHV010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56003_length_2590_cov_4.470611"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1935)
                     /locus_tag="PLQ29_09835"
     CDS             complement(73..1935)
                     /locus_tag="PLQ29_09835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HPG86988.1"
                     /translation="MNPATTSSGPAWDLSGEYPAIASAEFAADLAAVKDAIASLEADR
                     PSLAVYVDAAAGLDPVADSGVPASLARTMDRVDEAGATLRNLSVYVNCVLSTNGSDAE
                     AAAMRGYLASLRAALGAAEAPLSLVLKLCPEPFFAAFCALPGMARFRFALSQERKTRA
                     RALPLPAERAVATMSADGLHAWGGLYDAITGTLRCRALGPDGAESTVGLAKAASLLKR
                     PEPRVRESAWRGINAALSEHRHSLAAILNSIAGWRGAELSMRSHTEPGHYLDESLRQN
                     RLSSATLEAMMGVIRESRELGRRALRAQAGLYGVRRIGPWDILAPAPAAEGTNAVGLP
                     FERGLDTVRRAYASVHPSMGDFVSRMARSGRVEGRVEDGKRPGAYCTEFPKSRNQFVY
                     TTYQGALSELSTLAHELGHAYHCEAMRDIPLAESEYPMALAETASTFAETVLGEMLAS
                     EAGNDAERLELAWSDAQDAATFLVNIPARFEFESRFYERRPSGPVGPDELCAIMRDAW
                     AEWYGDALSEYDQWYWASKLHFHKTAVSFYNYPYSFGYLFSLGVYARRPELGERFHEA
                     YVAMLRDTGRMEVEELAFRHLGVDLSRPDFWRSSVAIIEQKVERFEELSRHAAP"
     gene            2007..2552
                     /locus_tag="PLQ29_09840"
     CDS             2007..2552
                     /locus_tag="PLQ29_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HPG86989.1"
                     /translation="MSRAFVGDDQYDAADDEAPELKIPVPPGSRNYLTPDGAAALADE
                     LRELETERRPRLLAELNRLGASVSEASPDELSRARRELGRVNRRLEYLGRMASMAETI
                     APPDGGYDRVRFGAGVTVEEEGGAVSAYRIVGIDEADPERGLVGWTSPIARALMGKRP
                     GDEATVALPEGTRRLMIVSIG"
BASE COUNT          402 a          843 c          982 g          363 t
ORIGIN      
        1 tcgccgacag gcgttgtacc tgactcgccg cgctggcggg ttaacggcgg cggctggtag
       61 gtagcccgag catcaagggg cggcgtgtcg cgacagctcc tcgaatcgct ctaccttctg
      121 ctcgatgatc gcgacgctgg agcgccagaa gtcgggccgg gataggtcga cgccaaggtg
      181 gcggaaggcc agctcctcga cctccatgcg gccggtgtcg cggagcatgg cgacgtaggc
      241 ctcgtggaag cgctcgccga gttcgggccg gcgggcgtag acgccgaggc tgaacaggta
      301 accgaaggag tacgggtagt tatagaagct gacggccgtc ttgtggaaat gcagcttgga
      361 agcccagtac cactggtcgt actcgctcag ggcgtcgccg taccactcgg cccaggcgtc
      421 gcgcatgatg gcgcacagtt cgtcagggcc gacgggaccg gacgggcggc gctcgtagaa
      481 gcggctctcg aactcgaaac gtgcgggtat gttgacgagg aaggtggccg cgtcctgggc
      541 gtcggaccag gccagctcga gccgctcggc gtcgttcccg gcctcggagg cgagcatctc
      601 gccgaggacg gtctcggcga aggtgctcgc ggtctcggcc agcgccatcg gatactcgga
      661 ctcggcgagc gggatgtccc gcatcgcctc gcagtggtag gcgtggccca gctcgtgggc
      721 cagcgtcgac agctccgaca gggctccctg gtaggtggtg tagacgaact ggttgcggga
      781 cttggggaac tcggtgcagt aggcgcccgg ccgctttccg tcctcgacgc ggccctcgac
      841 ccggccggaa cgggccatgc gcgagacgaa gtcgcccatc gacggatgca cggacgcgta
      901 ggcccgcctg accgtatcga ggccgcgctc gaagggaagg cccacggcgt tcgtcccttc
      961 ggccgcggga gcgggggcca ggatgtccca ggggccgatt cgccggaccc catagaggcc
     1021 ggcctgggcg cgcaaggccc tgcggcccag ctccctggac tcgcggatca cgcccatcat
     1081 cgcctcgagc gtggcgctgg agaggcggtt ctgcctgagc gattcgtcga ggtagtggcc
     1141 cggctcggta tgcgagcgca tggatagctc ggcgccgcgc cagccggcta tcgagttgag
     1201 tatggcagcg aggctgtggc ggtgttccga cagggcggcg ttgatgccgc gccaggccga
     1261 ctcgcggacc ctgggctccg gccgcttgag gaggctcgcc gccttggcca ggccgacggt
     1321 cgactcggcg ccgtccgggc cgagcgcccg gcaacgcagg gtgccggtga tggcgtcgta
     1381 cagcccgccc caggcgtgga gtccgtcggc cgacatcgtc gcgacggcgc gctcggccgg
     1441 cagcggcaag gcgcgggcgc gagtcttgcg ctcctggctc agggcgaatc ggaagcgcgc
     1501 catgcccgga agggcgcaga acgcggcaaa gaacggctcg gggcatagct tgaggacgag
     1561 ggagagcggc gcctcggccg cgccgagcgc tgcccggagc gaggcgaggt agccgcgcat
     1621 ggcggccgcc tcggcgtcgg agccgttcgt cgacaggacg cagttgacgt agaccgacag
     1681 gttgcgcagc gtcgcgccgg cctcgtcgac ccggtccatc gtccgcgcca gcgaggccgg
     1741 gacgcccgaa tcggctaccg ggtcgagccc ggcagccgcg tcgacgtaaa cggccaggga
     1801 cggccggtcg gcctcgagcg aggcgatagc gtccttgacg gcggcgaggt cggcggcgaa
     1861 ttcggccgag gctatggccg gatactctcc ggagaggtcc caggccgggc cggaggacgt
     1921 ggttgcggga ttcatgcggg cgactgtagc acggcgtccg ccgccgggca agccgaaacc
     1981 gtggtatagt cgctatgggg gagaccatgt ccagggcatt cgtgggagac gatcagtacg
     2041 acgcggccga cgacgaggcg ccggaactca agatccccgt cccgccgggc tcgcggaact
     2101 acctgacccc ggacggcgcg gcggctctgg ccgacgagct ccgggagctg gagaccgagc
     2161 gccgccctcg cctcctcgcc gagctcaacc gcctcggagc gtccgtctcg gaggcttctc
     2221 cagacgagct gtcccgggcc cgccgcgagc tcggccgagt caaccgccgg ctcgagtacc
     2281 tcggtaggat ggcctcgatg gctgagacga tagctccgcc agatggcggc tacgaccggg
     2341 tacgtttcgg cgccggcgtc acggtcgagg aggagggcgg cgccgtatcc gcttaccgca
     2401 tcgtcgggat cgacgaggcc gacccggaac gaggcctcgt cggctggacc agccccatag
     2461 ccagggccct catgggcaag cggcccggcg acgaggcgac ggtcgccctg cccgaaggga
     2521 cgaggcgcct catgatcgtg tcgataggat agtcgcgtcg gaaggacgcg atgcgtcgga
     2581 aggacgcgat
//
LOCUS       DAOPHV010000391         2585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_56166_length_2585_cov_4.912648, whole genome shotgun sequence.
ACCESSION   DAOPHV010000391 DAOPHV010000000
VERSION     DAOPHV010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56166_length_2585_cov_4.912648"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="PLQ29_09845"
     CDS             complement(<1..1081)
                     /locus_tag="PLQ29_09845"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPG86990.1"
                     /translation="MDYRRAKTTVSAVSWAIAALAFAVIVFIASARSGIVGTAVSGVA
                     AFVMVFIARRAISSALDERLYRLILGRGVSKLLMDFVERIRTCFTMPEFVSAIRESLE
                     LSMDASVVLIRSNTWEVVYSSPAALTADPGLVPNLKRNFRELSEGLVFMDERLNLTVS
                     SQGARGFFVLCRGYYLFVFSRLCPYADHDAYRVLYGELLIFFDRVLTVAKLFDIAALT
                     KEWRQIAETQRTFLPEMLPEQPKLTLAAYWRPLVNVSGDFYDAIRIDDDRTLLVMGDV
                     SGKGLAAALVMGVVINTIRAAGDKADLAALIRSCDRAIRGMGLEDKYVVMFLGLVDVG
                     AKRLRYVNAAMPDQYLVVRTVKGPVI"
     gene            complement(1339..2004)
                     /locus_tag="PLQ29_09850"
     CDS             complement(1339..2004)
                     /locus_tag="PLQ29_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86991.1"
                     /translation="MRRLGGAGYRGRRLGLHRSRGRGAAGGRGQDTAFHGIGGGRGER
                     PLFDAAAVSGAVRARGATVDSSRAPTTGSYRGSFRAADLTALIAGTEGLGAALDYRRA
                     PGRASLAFRLDRTTAPALVGLFPGIDAELLESLQPPALYDNPVSVAEYRSMLAGLLGQ
                     AALSVIDGQALELRVTLPGPVLELSGAAKAAPGGRSVSISVPALEAMVLAEPVAFRVE
                     WSE"
     gene            2274..>2585
                     /locus_tag="PLQ29_09855"
     CDS             2274..>2585
                     /locus_tag="PLQ29_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPG86992.1"
                     /translation="MIERTPLKILDQYRGKAFTGQWPTLPELFRMTASRYPDSPCFTV
                     YDPDRTTLSYAEALTSIEGLASFLAAEGVRKGDKIALTGKNSPEWAVCYLAILFAGAT
                     VV"
BASE COUNT          526 a          821 c          828 g          410 t
ORIGIN      
        1 tgatgacggg ccccttgacg gtgcgcacga ccaggtactg gtccggcatg gccgcgttaa
       61 catagcgcag gcgcttggcc ccgacgtcga ccaggcccag gaacatcacc acgtacttgt
      121 cctcgaggcc catgccgcgg atggcccgat cgcaggagcg gatgagcgcg gccaggtcgg
      181 ccttgtcgcc ggccgcgcgt atcgtgttga tcacgacgcc catgacgagg gcggcggcga
      241 ggcccttgcc ggatacgtcg cccatcacga gcagcgtacg gtcgtcgtcg atgcgaatgg
      301 cgtcgtagaa gtcgcccgaa acgttgacca gggggcgcca gtacgccgcg agcgtcagtt
      361 taggctgctc cggcaacatc tcaggcagga aggtacgttg cgtctcggct atctgccgcc
      421 attccttggt cagagcggct atgtcgaaca gcttggccac ggtaagcacg cggtcgaaaa
      481 agatcaggag ctcgccgtac agcacccggt aggcatcgtg gtcggcatag gggcagagcc
      541 gcgagaacac gaacagatag tagcccctgc agagcacgaa gaagccgcgc gcgccctggc
      601 tggataccgt caggttgagt cgctcgtcca taaacacgag accctcggaa agctcgcgga
      661 agttacgctt aaggttcggt accaggccgg ggtcggcggt cagcgccgcc gggctcgagt
      721 agacgacctc ccaggtattg ctgcggatca gcacgaccga ggcgtccatg gacaattcga
      781 ggctttcccg gatcgcggat acgaactcgg gcatcgtgaa gcaggtccgg atccgctcga
      841 cgaagtccat aagcagcttg gaaacgccgc ggccaaggat caggcggtac aggcgctcgt
      901 ccaacgcgga cgagatcgct cgccgagcta tgaataccat gacgaaggcg gccacgccgg
      961 agacggccgt accgacgatg cccgaccgcg cgctggctat gaagacgatg acggcgaacg
     1021 ccagcgccgc gatcgcccac gatacggccg aaaccgtagt cttggctcta cgataatcca
     1081 agtaaaccac ccctacgata gagcttggaa gcgtctcatg gtctcacagg ccgcggtcgc
     1141 tgtcaatcga agcggcaaaa aattatggta ggaagtggaa aagacaatcc ggtcggctac
     1201 tcgcctcgtg gcatgaacct tattggccta tcgtccataa ttgttttaca gagctacaaa
     1261 tagacgccaa actcgttagc agtcggtacg gttgaggcag tactggaagc ggaccaacac
     1321 tcttcgtaac actcgaagct actcggacca ctcgacgcga aaggcgaccg gttccgccag
     1381 caccatcgcc tccagggccg ggactgaaat cgacaccgag cgccctccgg gcgcggcctt
     1441 cgcggcgccg gacagctcga gcacgggccc gggcagcgtt acgcgtagct ccagggcttg
     1501 cccgtcgata acgctaagcg ccgcctgccc gaggaggcca gccagcatcg agcggtactc
     1561 ggcgacgctg acggggttat cgtagagcgc cggcggttgc agcgactcga gtagctcggc
     1621 gtcgatgccc gggaacagcc cgacgagggc cggcgcggta gttcgatcga gcctgaacgc
     1681 gagtgaggcc cggccgggag cgcgccggta gtcgagggcg gccccgagcc cctccgtgcc
     1741 ggctatgagc gcggtcaggt cggccgccct gaaggagcct cggtacgagc ccgtggtcgg
     1801 ggctctggac gaatcgaccg tcgctccacg agccctgacg gcgccggaga cggccgcggc
     1861 gtcgaaaagc gggcgctcgc cgcggccccc gccgatgccg tgaaacgccg tatcctggcc
     1921 tcgaccgccg gcggcacctc gaccccgaga ccgatggagg ccgagccgcc ggccccgata
     1981 gccagctcca ccgaggcgcc gcacgagact gctagtagcg ccatgagggc gaaggcgggt
     2041 agagcgagaa tccttgacga ggctatggct cttcgcatcg tagctcctcg ggccgacggt
     2101 atgaccggcg cagtatatgc gttggcgacg atataataca acggcgcacg gcgacggtta
     2161 catcgcctcg acgttatcat aaaaaacgtc gcgttccgcg caacctccgg ttttccaggg
     2221 tgtctatatg cacggtatgc tatgctctct catggctttt ggaggaaaac gatatgatcg
     2281 aacgtacccc gctgaagatc ctcgaccaat ataggggcaa ggccttcacg ggccagtggc
     2341 ccacgctacc cgagctcttc aggatgacgg cgtcccgtta tcccgacagc ccttgcttca
     2401 ccgtctacga tcccgacagg accacgctaa gctatgcgga agcgctaacg agcatcgagg
     2461 gcctagcctc cttcctcgcg gcggagggcg tacggaaagg cgacaagatc gcgctgaccg
     2521 gcaagaactc gcccgagtgg gcagtctgct acctggccat cctcttcgcc ggcgcgaccg
     2581 tcgta
//
LOCUS       DAOPHV010000392         2579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_56323_length_2579_cov_4.149762, whole genome shotgun sequence.
ACCESSION   DAOPHV010000392 DAOPHV010000000
VERSION     DAOPHV010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56323_length_2579_cov_4.149762"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..715
                     /locus_tag="PLQ29_09860"
     CDS             242..715
                     /locus_tag="PLQ29_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86993.1"
                     /translation="MNDSETRAFKNNKVAKLVGLLPFAAGCDDAMRSSLSHLATFVIA
                     NRGRARRFFDHAPADDADPMARLRTIADFKGGDSAALEQGLARLALCMTSGYRRDAEI
                     DRLTGEYNPVNGDTVEPERSAVSSCSEKNGSAVIDSILTADEAIYTFWETPDEQR"
     gene            702..1421
                     /locus_tag="PLQ29_09865"
     CDS             702..1421
                     /locus_tag="PLQ29_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPG86994.1"
                     /translation="MSNDKLRSTGFLFAAFFLGGFMINFVSDIGKHGFFWSTTNPYQL
                     AQLAICAAFILSALVRPLRILQPIVFLVFSPVSIIQSTRNIYGLGFFIIGILLLERTG
                     FYARHRLPKLILSISYLLAIEIVGVVSSGKSPMEASAPTFFIVAFGVFLWFLYKDRLV
                     IILKEPKPKLSLAEKGLSTAERAFIGQTLSGKSQKEISIDFELSESTVRNTLARAYKK
                     LDIEDRVGLAVLGERFEIDSD"
     gene            complement(1428..2405)
                     /locus_tag="PLQ29_09870"
     CDS             complement(1428..2405)
                     /locus_tag="PLQ29_09870"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPG86995.1"
                     /translation="MFHPEEVIFAPTGRCNLRCAHCRVRSDGPELAIEDAVSFLESCS
                     AAGIERVGFSGGEPFLRLDFIVAVTAAAVDRGLYFDRLMTNGDWWTTGASLRAALGAV
                     AEAGFDGVIGLSHDAYHGQAPERVAAFMAAAYEAFGRRDAVELLSVRSPDDGAFFRGL
                     ETVATALGGRLEYELSEPARIVDEAYLARSEADPDDGSALVAQVIRSPRSRSAEEGAW
                     DAERWFVDDFCEGPGNVLYVHPDGAVAVCCGFANENPQLIVGSVGDSVEAIVAEAAAR
                     PLVRACYEAGLATTRRRLEAAGVRFPGKTGDACFFCDYLCKRGLVGSRG"
BASE COUNT          486 a          844 c          793 g          456 t
ORIGIN      
        1 aagagaaagg tagctcgata cggctgtccg cctgcgggct tcatgcggcc atgatacgtc
       61 gaagcgcctc gtccgctcaa gcgccagaat ccagtttccg tcggctacgg ttccgaacgg
      121 agatggtaca aaaaaatgcc ttgacgcccc atcgattctc tcgaatactc agcgcatgaa
      181 ctttgcagca atcgattcct tgtggaatcg tttatccgat gtttcggccg aagccctccg
      241 catgaacgat tcggaaacac gcgccttcaa gaacaacaag gtagccaagc tcgtcgggct
      301 cctgcccttc gcagccggct gcgacgacgc gatgcgctcg tcgctcagcc acctggcgac
      361 cttcgtgatc gccaaccggg gccgcgcccg ccgattcttc gaccacgcgc cggccgacga
      421 cgccgacccg atggcccgtc tccgcaccat cgcggacttc aagggaggcg actcggcggc
      481 gctcgagcag ggcctggcgc gcctcgcgct atgcatgacg agcggctacc gcagggacgc
      541 cgagatcgac cgcctgacgg gcgagtacaa tcccgtaaac ggcgatacgg tcgagcctga
      601 gcgatcggcc gtttcatcgt gctcggaaaa gaatggatca gccgtcatcg attcgatcct
      661 aacggctgat gaagctatat atacattttg ggaaacacca gatgagcaac gataagctac
      721 gttcgacagg gtttctattc gccgcgttct tccttggcgg attcatgatc aacttcgtct
      781 ccgacatcgg gaagcatggt ttcttctggt cgacgacgaa cccctaccaa ctcgcccagt
      841 tggcgatatg cgcggcgttc attctaagcg ccttggttag gccgcttcga atcctacagc
      901 ctatagtctt cctggtcttt agccccgttt ccattatcca aagcacgaga aacatctatg
      961 gactcggttt ttttatcatc ggcattctgt tgctcgaacg caccggcttc tacgcgaggc
     1021 atcgcttgcc taagctgata ctcagcatca gttatctcct cgctatagaa atagtcggcg
     1081 tcgttagtag cggcaaatct ccgatggaag cgagcgcccc taccttcttc atcgtcgcgt
     1141 tcggcgtctt cctgtggttc ctttataagg ataggctcgt tatcatcctc aaggagccga
     1201 agcccaagct atcgctggcg gagaagggac tatcgacggc cgagcgggcg ttcataggcc
     1261 agacgctctc cggcaagtcg cagaaggaga tatccatcga cttcgagctg tccgagtcga
     1321 cggtcaggaa cacgctggcc cgggcctaca agaagctaga catcgaggac cgcgtcggcc
     1381 tggccgtgct cggagaacgc ttcgagatag actcggactg aggttcgtca cccacgagag
     1441 cctacgaggc ctcgcttgca caggtagtcg cagaagaagc aggcgtcgcc ggtcttgcca
     1501 gggaaacgga ctcccgccgc ctcgagccgc ctcctcgtcg tggcgaggcc ggcctcgtag
     1561 caggcgcgca cgagcgggcg ggcggccgcc tcggcgacaa tcgcttctac ggagtcgccg
     1621 accgagccga cgataagctg cgggttctcg ttggcgaagc cgcagcagac cgccacggcg
     1681 ccgtcgggat ggacgtacag cacgttaccg gggccctcgc agaagtcgtc gacgaaccat
     1741 cgttcggcgt cccaggcgcc ttcctcggcc gacctcgagc gcggggatct gatgacctgc
     1801 gcgacgaggg ccgagccgtc gtcagggtcg gcctccgacc gggccaggta agcctcgtcg
     1861 actatcctgg ccggctcgct tagctcgtat tccagtcggc cgcccagggc cgtagcgacc
     1921 gtctctaagc cacggaagaa cgccccgtcg tcgggtgagc gcaccgacag gagctccacc
     1981 gcgtcccggc ggccgaaggc ctcgtaggcc gccgccatga aggccgcgac ccgctcgggc
     2041 gcctggccgt ggtacgcgtc gtggctcagg ccgatgacgc cgtcgaagcc ggcctcggcg
     2101 accgcgccga gcgccgcgcg tagcgacgcc ccggtcgtcc accagtcgcc gttggtcatc
     2161 agccggtcga agtacaggcc ccggtccacc gcggcggcgg tcaccgcgac gatgaagtcg
     2221 agcctcagga agggctcgcc gcctgagaag ccgacgcgtt cgatgccggc cgccgagcat
     2281 gattcaagga aggagacggc gtcttcgatc gccagctccg ggccgtcact ccgcacgcgg
     2341 caatgggcgc aacgcaggtt gcatcggccc gtaggcgcga agatcacttc ttccgggtgg
     2401 aacataggac tatagcctac tcgtcctcgg cgccatgctc aagtgcccgg gcgggcctcg
     2461 cgggcaaggc ggcgggcgac agggccaggg cgccgaggat agccgccagc gccccgaagg
     2521 ccgctggcag gccgatgccg gcgagcctac cgacgccgac gttgacgagc gccgcgatg
//
LOCUS       DAOPHV010000393         2575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_56434_length_2575_cov_5.387302, whole genome shotgun sequence.
ACCESSION   DAOPHV010000393 DAOPHV010000000
VERSION     DAOPHV010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56434_length_2575_cov_5.387302"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1158)
                     /locus_tag="PLQ29_09875"
                     /pseudo
     CDS             complement(1..1158)
                     /locus_tag="PLQ29_09875"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(1336..2238)
                     /locus_tag="PLQ29_09880"
     CDS             complement(1336..2238)
                     /locus_tag="PLQ29_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86996.1"
                     /translation="MSLRSLARELAISCGSVQNRMDRLSRQGIALHSQFRPRAALREN
                     VCFDGLVSFDRSQYYPSDIGLSISSSSRFILGLSHATTRRSGTLSVAQKNHLESTYEG
                     DEFEMKAVERSFAEHLDLLAIENRDAQGHPLIIVTDEKLEYVRALHAHPLYLDQDERH
                     RCAQLRVPSTMPRTHANPLFASNYLDREIRKDQANHRRESTCYSRNAANCMARLASHL
                     VWHNYMKRYLINWPVDRTETHAEMAGIDHGALKRGRVMMFTKRAFLSRLNLVAIDLWL
                     WMKRVYDPIAGHPTIGYLPKYAFG"
     gene            2300..2428
                     /locus_tag="PLQ29_09885"
     CDS             2300..2428
                     /locus_tag="PLQ29_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86997.1"
                     /translation="MEKVCTEKVRLQEVQRNRGTDPNALVWCDPMDWYHRSLGGSK"
BASE COUNT          503 a          722 c          784 g          566 t
ORIGIN      
        1 ggcaacatgg aggtgccgcg cttcagccgt atcgcgcgct cttatcttct ccatatcggc
       61 aatctcgact ggacgaagat caaccccgac atcttcggct cgatgatcca ggccgttgcc
      121 gatgacgagg agcgcggctc gctcgggatg caccacacca gcgtcccgaa catcctgaaa
      181 gtgctcaatc cgctgttcct cgacgatctg cgggagcggc ttgccgttct cgatgaaaaa
      241 ggcgttatcg ccgacgacgt acgcgtacgg gtagtcgacg cttttcatct cggcgtcgac
      301 ctgcaggcgg ccgcggccgc gctcgccctt ggtcagatcg aggccggcag cgaaggagct
      361 acccttgaca ccgcaggtcc agatgaaggt atcggtctcg atgaccgtac cgtccttgca
      421 tagcaccttg ccgggctcgg cgccgatgat cgacgcgttg agcatgacct cgcagcccat
      481 tttggccagg tacttctccg ccttgacgcg gagcggctcc tcgaggatgg gcaggattga
      541 cggcagggcc tcgatattga cgacgcggga ctcggagcga tccagataat gtttgcggca
      601 catcgcgtca cggtattcga gcagctcgcc gatcatttcc atgccggtga agccggcgcc
      661 ggcgacgacg aaggtgagca gacgcctacg gacggcggga tcggtctcct cggcagcttt
      721 ggcgaacatg tgctcgacgt ggtggcggag gcgcatcgcg tcgtcgaacg accagagact
      781 gaaggagttc tccttgacgc caggtatatt gaattactcg ggctcggcac ctgccccgac
      841 gattatgtag tcgaaatcgt acgagcgagc cttgccgacg gccttcttgc ccttgaagtc
      901 gaccgatacg acctcgtcga ggaccacgtc gatcttcttg gcgccgaata ttttcttgaa
      961 atgcacctgg accgactcgg gctcggtgcg ccagccagct acctcgtgta gttcggtcat
     1021 caacgtgtgg aacggctttc tatcgatgag cgtgatctcc acgccgctgt ccttgcggaa
     1081 acgcttctca agtatctttc cggcccagac gccgccgtac cctcctccga ggattactat
     1141 cttcttcgct tcggccatag tctctccttg ccagcctagt gtaatgcgga atggtacccg
     1201 cgacgctaaa atttatcatc gcgtgacgga aaacgactaa tatgagttat gtatacatag
     1261 ttataggaaa atgtatatgt ttggcaagcc ctggctagtg ttacgaatct taagcagtct
     1321 cccgccactc gccgatcaac cgaaggcata cttggggaga tatccgatcg tcgggtgtcc
     1381 tgcgatcggg tcgtagacgc gcttcatcca cagccaaagg tcgatagcga ccaagttcaa
     1441 acgtgagagg aatgctcgtt tcgtgaacat catcacccgt ccacgcttca gcgcaccgtg
     1501 atcgatacct gccatctccg cgtgcgtctc ggttctatct actggccagt taatgagata
     1561 gcgcttcatg tagttgtgcc acacgaggtg cgaagcgagc ctcgccatgc agttggcggc
     1621 gttgcggctg tagcaggtgc tctctcgtcg atggttcgcc tgatccttgc ggatctcccg
     1681 gtcgaggtag ttggacgcga acagcgggtt tgcatgggtg cgcggcatcg tcgacggtac
     1741 tcgtagctgg gcgcagcgat ggcgttcatc ctgatcgagg tacagcgggt gcgcatggag
     1801 cgcgcggacg tactcgagct tctcgtcggt gacaatgatg agcggatgac cctgagcgtc
     1861 gcggttttca atagcaagta gatccaggtg ttccgcaaac gatcgctcga cggccttcat
     1921 ttcgaattca tcaccctcgt aggtcgactc gagatggttc ttctgggcca cggacagcgt
     1981 gcccgagcgc ctggtcgtcg cgtggctcag gccgaggatg aagcgggagc tcgaggagat
     2041 cgagaggcca atgtcgctcg ggtagtattg cgagcggtcg aaggagacga ggccgtcgaa
     2101 gcacacgttc tcacggagag cggctctggg tcgaaactgg gagtggagcg caattccttg
     2161 cctggaaagg cgatccattc gattttggac gctaccgcag gagatggcca gctcgcgggc
     2221 gagcgagcgc aggctcatcg atgacgaggc gaggctttcg atccgctcgt atggcaggac
     2281 cttcttcgcg taatagtcga tggagaaggt ctgtaccgag aaggtgcgcc tgcaggaggt
     2341 gcagcggaac cgaggtaccg atccgaacgc cttggtctgg tgcgatccga tggattggta
     2401 ccatcggtcc ctcggtggct cgaagtgatg ttcgcagtcc ttgtaggggc agaatggtgg
     2461 ttcgaacgta tgggacctcc atgagcttgg gataggttgt acgacctaca cgctcgcgcg
     2521 tatcaccatg ctttcgatgc tatccgtggt tttgatccat aagattcgga acact
//
LOCUS       DAOPHV010000394         2567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_56693_length_2567_cov_3.187500, whole genome shotgun sequence.
ACCESSION   DAOPHV010000394 DAOPHV010000000
VERSION     DAOPHV010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56693_length_2567_cov_3.187500"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..996)
                     /gene="rarD"
                     /locus_tag="PLQ29_09890"
     CDS             complement(43..996)
                     /gene="rarD"
                     /locus_tag="PLQ29_09890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HPG86998.1"
                     /translation="MGDDRPGRARTESVGMLFGIGAYGLWGLFPLYWKRLSSVPSLQI
                     LMHRIAWAFALTFVLALLLGKRRLMAELLRERKRLAATIAAGFLVSLNWGIYIWAVNA
                     GHIVETSIGYFLNPLVSVALGSFVFKEKIDRGIKAACAIAAVGIAVLTVSYGKLPWIA
                     LSLAATFSLYGLIKKLTNLDGLTGLAMETAPVMPFALGYLILEELAGRGAFGRSGGIE
                     TSMLVLAGAVTALPLFFFAEGVKRVPLSRMGFLQYISPTLQLALGLFVFGETLSAAKA
                     AAFAFIMAALVVFAATRPRSTGGPSPALPPTARGPKRPGRG"
     gene            complement(1068..1238)
                     /locus_tag="PLQ29_09895"
     CDS             complement(1068..1238)
                     /locus_tag="PLQ29_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG86999.1"
                     /translation="MSKAHRGKGIADQPDGGRGICPVCKRGGVKLMYEQEIEGQKTKI
                     CKMCKATIANKK"
     gene            complement(1252..1824)
                     /gene="rplQ"
                     /locus_tag="PLQ29_09900"
     CDS             complement(1252..1824)
                     /gene="rplQ"
                     /locus_tag="PLQ29_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPG87000.1"
                     /translation="MNHKKGFNKLNRNASHRKALLRNMATVLFKHERIVTTKAKAIEV
                     RRVAEKMITRAKEDSVHNRRIVGEKITDQAILAKLFTDIGPRMKDRNGGYTRILKIGF
                     RDQDAASMVVLELVDHKLDGDKKADKKKPEAKAKKAEPKAASKAKKPDAKADGKAKSP
                     RATKKAAAAKQSEPESAESAKSAESAKSAE"
     gene            complement(1814..>2567)
                     /gene="rpoA"
                     /locus_tag="PLQ29_09905"
     CDS             complement(1814..>2567)
                     /gene="rpoA"
                     /locus_tag="PLQ29_09905"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPG87001.1"
                     /translation="RLQLPDDEEQETFVYEFVGPAEIKGADLMKDKALKVFNPTTHIM
                     SMMDGVKLEMELQVDLGRGYVPSEVNEKYIEIVGTIPMDAIFSPVSKARYSVEPTRVG
                     HRSDYDKLVLEVWTDGTLKPEDALAEAAKIAKDHFSIFVNFDEGEPNMDEESDELDEK
                     IKAILTTPVEELELSVRSSNCLKNANIKTIGDLTRKTEDDIAKTRNFGKKSLQEIKDR
                     LKEWNLGLGMTDYAELREHLKLEKQKEDIHEP"
BASE COUNT          431 a          830 c          765 g          541 t
ORIGIN      
        1 ggacagtcag gctggaaagc ctgacctaca cgtcggcgcc gctcagcctc gcccgggcct
       61 cttcggccca cgggctgtcg ggggcaaggc cggcgaaggt ccgccagtgg acctcggcct
      121 cgtcgcggcg aacacgacca gagccgccat gatgaacgcg aaggcggccg ccttggccgc
      181 cgacagcgtc tccccgaaga cgaaaaggcc gagcgccagc tgcagggtcg gcgatatgta
      241 ctggaggaat cccatcctcg atagcggcac gcgcttgact ccctcggcga agaagaacag
      301 cggcagggcg gtcaccgccc cggccagcac cagcatggac gtctcgatgc ctccggaccg
      361 accgaaggcc ccgcgaccgg ccaactcctc gaggatcagg tatccgagcg cgaacggcat
      421 gacgggcgcc gtctccatcg ccaagcccgt cagcccgtcg aggttcgtca gcttctttat
      481 caggccatag agcgagaaag tggcggcgag cgacagcgct atccagggca gcttgccgta
      541 cgataccgtc agcacggcta tcccgaccgc cgcgatcgcg caggccgcct tgataccccg
      601 gtcgatcttc tccttgaaca cgaaagagcc gagggcgacg gagacgagcg ggttgaggaa
      661 gtaccctatg ctagtctcga cgatatgtcc ggcgttgacg gcccaaatgt agatacccca
      721 gttcaggctg acgaggaagc cggccgcaat cgtcgccgcc aggcgcttac gttcgcgcag
      781 tagctctgcc atcagccgcc gcttgccgag tagcaaggcc agcacgaagg tcagcgcgaa
      841 ggcccaggct atgcgatgca tcagtatctg tagcgacggc accgacgaca aacgtttcca
      901 gtacagcggg aacaggcccc agagcccgta ggcgccaatg ccgaatagca tcccgacgga
      961 ctcggttcga gcccttcccg gcctatcgtc acccatggta cctccatatt aaaaaaagga
     1021 ggccgctagc gagcggcctc cccgtacgac gcggtccgcg cgccgcctta tttcttgtta
     1081 gcgatcgtag ccttgcacat cttgcagatc ttggtcttct gcccctcgat ctcctgctcg
     1141 tacatcagct tgacgccgcc acgcttgcag accggacaga tcccgcgacc gccgtcgggc
     1201 tgatcggcta ttcccttacc gcgatgggcc ttggacatgg tgcttcctcc cttactcggc
     1261 cgacttggcc gattcggccg acttggccga ttcggccgac tcgggctcgg actgcttggc
     1321 cgctgcggcc ttcttcgtcg ctcgaggcga cttggccttg ccgtcggcct tggcgtcagg
     1381 cttcttggcc ttcgaagcgg ccttgggctc ggccttctta gccttcgcct cgggcttctt
     1441 cttatcggcc ttcttgtcgc cgtcgagctt atggtcgacc agctcgagca cgaccatgct
     1501 ggccgcgtcc tggtcgcgga acccgatctt caggatgcgg gtgtagccgc cgttgcggtc
     1561 cttcatcctg gggccgatgt cggtaaacag cttggccaga atcgcctgat cggtgatctt
     1621 ctcgccgacg atgcgcctgt tgtggacact gtcttcctta gccctggtga tcatcttctc
     1681 ggcgaccctg cggacctcga tggccttggc cttggtcgtg acgatccgct cgtgcttgaa
     1741 gaggacggta gccatattcc ggaggagagc cttacgatga gaggcgttcc ggttaagctt
     1801 attgaagccc ttcttatggt tcatggatat cttccttctg cttctccagc ttcaagtgct
     1861 ccctgagctc ggcgtagtcg gtcatgccga ggcccaggtt ccattccttg aggcggtcct
     1921 tgatctcctg caaggacttc ttaccgaagt tcctcgtctt ggcgatatcg tcctcggtct
     1981 tgcgcgtcag gtcgccgata gtcttgatgt tggcgttctt gaggcagttc gacgaacgta
     2041 cggaaagctc aagctcttcg accggggtgg tcaggatggc cttgatcttc tcgtcgagct
     2101 cgtcgctctc ctcgtccatg ttcggctcac cctcgtcgaa attgacgaaa atcgagaagt
     2161 gatccttggc gatcttggcc gcctcggcga gcgcgtcctc cggcttgagc gtgccgtcgg
     2221 tccatacctc gagcacgagc ttgtcgtaat cggagcggtg cccgacgcgc gtcggctcga
     2281 ccgagtaacg cgccttgctg accggcgaga agatggcgtc catcggtatg gtaccgacga
     2341 tctcgatgta cttctcgttc acctcgctcg gtacgtagcc gcggccgagg tctacctgga
     2401 gctccatctc gagcttaacc ccgtccatca tgctcatgat gtgggtcgtg gggttgaaga
     2461 ccttgagcgc cttgtccttc atcaggtcgg cgcctttgat ctcggcggga ccgacgaact
     2521 cgtagacgaa cgtctcctgc tcctcgtcgt cgggtagctg caggcgt
//
LOCUS       DAOPHV010000395         2566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_56717_length_2566_cov_3.730785, whole genome shotgun sequence.
ACCESSION   DAOPHV010000395 DAOPHV010000000
VERSION     DAOPHV010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56717_length_2566_cov_3.730785"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PLQ29_09910"
     CDS             <1..612
                     /locus_tag="PLQ29_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87002.1"
                     /translation="GAADPEANDGLLARRLAELDEEARRAAALRDELARYGRACSRAR
                     LEVGRFEPDVLAAASYIFTIERGSFVGDSARLELSKRWTERLPFATFALYVPGEAFSG
                     VGEYHVSWGFGLGDDQPGSRAIVGSARAAGMDILTLEDRRCVRTAFYRDDPRDMRPGE
                     LSPIVDAFRAAGYEATGPAIGHLLEVDGAGGSARYLYALYIPV"
     gene            721..>2566
                     /locus_tag="PLQ29_09915"
     CDS             721..>2566
                     /locus_tag="PLQ29_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG87003.1"
                     /translation="MKNNAFRGLIVGLACVGIAAAALVGCGTTDSAGTAAYTPGTYTA
                     VGQGNFGDVEVSVTFSATKIVSITVGEHGETPGLSDPALEGIPAAIVKGQTLAVDTVS
                     GATHSSEAVLSAVADCVKQAGGDVEALKAKKGAKAAKGARIEKSADVVIVGGGGAGLA
                     AAVSAAQSGAKVVLIEKAAALGGNTIRSGGVQNAVDPARQKAVKMTAPLIGDLKSILD
                     AKEADFGEFAPVVATLKGQIRDYLASGSDVLFDSVELHMYHSYIGGKRVDLKGQEIHG
                     ELALVRTLAEGSPKAIEWLESLGLKFNDGIGTVLGALWPRTHSNTKPVGTGYVTVLSE
                     TAAKLGVEIMLETKAEELLVEKGRVVGVKATKADGAPVVLRASKGVVMATGGFGANPE
                     MRAKYNTYWPSMPLSMPTTNTADATGDGIVMGEKAGAKLVGMGFIQLMPSSHPVSGAL
                     SGGLWGSAETQVFVNKQGKRFVNEYAERDVLASAALKQTDGIFYIICDQDSAGNPQPG
                     SKNGWGDDVDTLIRNHDVYKADTLAGLEEQLGMPAGSLQAEIAKYNGYIASGTDPDFG
                     KRNFGAPLVKPPFYATIRSPSVHHTMGGLAIDASARVLDASGKAIPGFY"
BASE COUNT          434 a          884 c          844 g          404 t
ORIGIN      
        1 ggcgccgcgg accccgaggc caacgacggg ctcctggcca ggcgtctggc cgagctggac
       61 gaggaggcta ggcgggcggc ggccctgcgc gacgaactgg cccggtacgg ccgcgcctgt
      121 tccagggccc gcctggaggt cggacgcttc gagcccgacg tcctggccgc ggccagctat
      181 atctttacga tagagcgtgg ctccttcgtc ggcgactccg ccaggctgga gctatccaag
      241 cgatggaccg agcggctacc gttcgccacc ttcgcgctgt acgtgcctgg cgaggccttc
      301 tcgggagtgg gcgagtatca cgtctcctgg ggtttcggtc tgggcgacga tcagcccggc
      361 tcccgggcca tcgtcgggtc ggcccgcgcc gccggcatgg atatcctgac cctcgaggat
      421 cggcgctgcg tccgtaccgc cttctatcgc gacgatccgc gcgacatgag gcccggcgag
      481 ctgtccccca tcgtggacgc cttccgggct gccgggtatg aggccacggg gccggccatc
      541 ggacacctcc tcgaggtgga cggcgcggga ggctcggcgc gctacctcta cgcgctctat
      601 atcccggtat gattccgtaa acgcgatatc gatacttaat gctttatcta ttgaccttca
      661 agccgcttga ggctttaggc tcctgctcgc ggacgaatga gccgcaagga gcgaacggaa
      721 atgaagaaca acgcgttcag gggcctcatc gtaggcctcg cgtgcgtcgg tatcgcggcg
      781 gccgcgctgg tcggctgcgg taccaccgat tccgctggaa cggcggccta tacgccgggc
      841 acctatacgg cggtcggtca gggcaacttc ggcgacgtcg aggtcagcgt cacctttagc
      901 gccaccaaga tcgtctcgat aacggtcggc gaacacggcg agactcccgg cctgtccgat
      961 cccgccctgg agggcatccc ggccgccatc gtcaagggac agaccctggc cgtcgacacg
     1021 gtctcgggcg ccacccactc gagcgaggcc gtcttgtcgg ccgtggccga ctgcgtcaag
     1081 caggccggcg gcgatgtcga ggccctcaag gccaagaagg gcgcgaaggc agccaaggga
     1141 gccaggatcg agaagagcgc cgacgtcgtc atcgtcggcg gcggcggcgc cggcctggcg
     1201 gcggccgtgt ccgcggccca gtccggagcc aaggtcgtgc tgatcgagaa ggccgctgcc
     1261 ctcggcggca acaccatccg ctcgggcggc gtgcagaacg ccgtcgaccc ggcccgtcag
     1321 aaggccgtca agatgaccgc gccgctgatc ggcgacctca agtccatcct cgacgcgaag
     1381 gaagctgatt tcggcgagtt cgccccggtc gtggccaccc tcaaaggcca gatccgcgat
     1441 tacctcgcct cgggctccga cgtcctgttc gactcggtcg agctgcacat gtaccatagt
     1501 tacataggcg gcaagcgcgt cgacctcaag ggccaggaga tccatggcga gctggctctc
     1561 gtccggaccc tcgccgaggg ttctcccaag gccatcgagt ggctcgagtc gctcgggctc
     1621 aagttcaacg acggcatcgg caccgtgctc ggcgccctgt ggccgcgcac ccacagcaat
     1681 accaagccgg tgggcaccgg ctacgtgacc gtcctgtcgg agaccgcggc caagctcggc
     1741 gtggagatca tgctggagac caaggccgag gaactcctcg tcgagaaagg ccgggtcgtc
     1801 ggcgtcaagg ctaccaaggc cgacggcgcg ccggtcgtac tccgggcgtc caagggcgtc
     1861 gtgatggcca cgggcggctt cggggccaat cccgagatgc gcgccaagta caacacgtac
     1921 tggccgtcca tgccgctatc gatgccgacg accaatacgg ccgacgccac cggcgacggc
     1981 atcgtcatgg gcgagaaggc cggcgccaaa ctggtcggca tgggcttcat ccagctgatg
     2041 ccctcgtccc acccggtctc cggcgccctc tccggagggc tctggggtag cgccgagact
     2101 caggtcttcg tcaacaagca gggtaagcgc ttcgtcaacg agtacgccga gcgtgacgtg
     2161 ctcgcctccg ctgccctcaa gcagaccgac ggtatcttct atatcatctg cgaccaggac
     2221 tcggccggca atccgcagcc aggttcgaag aacggctggg gcgacgacgt cgacaccctg
     2281 atcaggaacc acgacgtgta caaggccgac accctggccg gcctcgagga gcagctcggc
     2341 atgcccgcgg gtagcctcca ggcggagata gccaagtata acggctatat cgcctccgga
     2401 accgaccccg acttcggcaa gcgcaacttc ggcgctccgc tggtcaagcc gccgttctac
     2461 gcgacgatcc gttcgccgtc ggtccatcac acgatgggcg gcctcgcgat cgacgcgtcc
     2521 gcccgcgtcc tcgatgcgtc cggcaaggcc atccccggct tctacg
//
LOCUS       DAOPHV010000396         2565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_56751_length_2565_cov_3.921116, whole genome shotgun sequence.
ACCESSION   DAOPHV010000396 DAOPHV010000000
VERSION     DAOPHV010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56751_length_2565_cov_3.921116"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /gene="mnmG"
                     /locus_tag="PLQ29_09920"
     CDS             <1..1520
                     /gene="mnmG"
                     /locus_tag="PLQ29_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPG87004.1"
                     /translation="HVFMDTVTGLVASADGARALGVVTERGSRISARAVVVASGTFME
                     GRVFIGEHRAPSGRLGEAAAVGLGSWLRAAGFPVGRMKTGTPARVKASSVDLGSLELQ
                     PGDSERRPFSFMNDEARGPELPCHITYTNERTHAIIRANIARSPLYSGDITGKGPRYC
                     PSIEDKVVKFPDRDRHQIFVEPEGLYTDELYLNGLSSSMPEDVQEAFIRTMAGFEGAY
                     VVRPGYAVEYDYVDPVDLYASLESKRLRGLFMAGQTNGTSGYEEAAAQGLVAGVNAAL
                     STAGEPPLVLSRADAYIGVLIDDIVTLGAKEPYRMFTSRAERRLALRHDTADRRLTPI
                     AMRVGLASKERAERFERKVEGMDAVRELLRARRVSQADAERDGAFSPHVGEDLARALA
                     DPHIAELDVETIEPAVSAYPRDWVQGVILDLRYEGYVAKEERLSARLGKLDAARLSPD
                     FDYAAVPGLSAESVEKLSATRPLSLGQASRIPGVRPSDVALLAVRLATWARPSRA"
     gene            complement(1644..2273)
                     /locus_tag="PLQ29_09925"
     CDS             complement(1644..2273)
                     /locus_tag="PLQ29_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87005.1"
                     /translation="METRMAMIKSALEIAMERTKNLKADPKALAAAEAKQEGKRLAGE
                     YLADPTACDFQSRFAAIAKDRREAAREGAFDVFVSRIQLPTSAVAQPEAELAPVAEGL
                     KALNVAPFGEKKIQATFEQLVGFLARYIEDAKRVEDAIRKQYAPRLKAKEQEMSARMG
                     RPVRIDPMQDPDFAAFYKQNVGQMRDQYQAALDQAKADLAKLCGIHKDD"
     gene            complement(2170..>2565)
                     /locus_tag="PLQ29_09930"
     CDS             complement(2170..>2565)
                     /locus_tag="PLQ29_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87006.1"
                     /translation="RLVGQTLMVLAVLLVKKRVTPRTSFLGKATVASTMALYAFELLR
                     FVADLPPAAYSALELVVGAVVAVSILDKLRIMLQDLRAAPVTAAGPGRLIPTNDGDTH
                     GDDQERARDSDGTNQEPEGGSQGPGGRRG"
BASE COUNT          388 a          877 c          870 g          430 t
ORIGIN      
        1 tccacgtctt catggacacc gttacgggac tcgtcgcgtc ggccgacggc gcgcgcgccc
       61 tgggcgtcgt gaccgaacgc ggcagccgca taagcgcgcg tgccgtcgtc gtcgcctccg
      121 gcaccttcat ggaaggccgc gtcttcatag gcgagcaccg cgctccttcg ggccgcctag
      181 gcgaagccgc cgccgtcgga ctcggttcct ggctacgggc cgccggcttc ccggtcggcc
      241 gcatgaagac cggcacgccg gctcgcgtca aggcgtccag cgtagacctg ggctccctcg
      301 agctccagcc cggtgactcc gagcggcgcc cgttctcgtt catgaacgac gaagcgcgcg
      361 ggccggaact accgtgccat atcacctaca cgaatgagcg tacccacgcg atcatacgcg
      421 ccaacatcgc ccgatctcct ttgtactccg gcgacatcac cggcaagggg ccgcgctact
      481 gcccgagcat cgaggacaag gtggtcaagt tccctgatcg tgaccgccac cagatcttcg
      541 tggagccgga gggcctctat accgacgagc tgtacctgaa cggcctgtcg tcatcgatgc
      601 ccgaggacgt gcaggaggcc ttcatcagga cgatggccgg cttcgagggc gcctacgtgg
      661 tcaggccggg ctacgcggtc gagtacgact acgtcgaccc cgtcgacctg tacgcgagcc
      721 tcgagtcgaa gcgtctacgc ggcctgttca tggccggcca gacgaacggc acctccggct
      781 acgaggaggc cgcggcccag ggcctcgtgg ccggcgtcaa cgccgcgctg tcgacggcag
      841 gcgagcctcc gctcgtgctg tccagggccg acgcgtacat cggtgtgctg atcgacgaca
      901 tcgtgaccct tggcgccaag gagccgtacc gcatgttcac gagccgggcc gagcgtcgcc
      961 tggccctgcg ccacgacacc gccgaccgtc gcctgacccc gatagccatg cgcgtcggtc
     1021 tggcttccaa ggagcgcgct gagcgtttcg agcgcaaggt cgagggcatg gacgccgtgc
     1081 gcgaactcct gcgcgcgcgc cgggtgtcgc aggctgacgc cgaacgcgac ggagccttct
     1141 cgccgcacgt cggggaggac ctggccagag ccctggccga tccgcatatc gcggagctgg
     1201 acgtggagac gatagagccg gctgtatcgg cctatccgcg cgattgggtg cagggcgtga
     1261 tcctggacct gcgctatgag ggctacgtgg ccaaggaaga gcgcttatcg gctagactcg
     1321 gcaagctcga cgccgccagg ttgtcacccg acttcgatta cgccgccgta cccggcctgt
     1381 ccgccgagtc ggtcgagaaa ctgtccgcta ccaggccgct atcgctcggt caagcctcgc
     1441 gtataccggg cgtcaggccg agcgatgtgg cgctactggc ggtcaggctg gcgacctggg
     1501 ctcgcccgag tcgtgcctga gaccggcaac cgggatgcgg ggcggtcgca cctgaggccg
     1561 gcggccgggc cgaagcgacg gcctacgcag ggtctcgggc gcggggcgcc cgaggggcgc
     1621 ggacgcgccc gggggtagcg gtcctagtcg tccttatgaa taccgcacag cttggcgagg
     1681 tcggccttcg cctggtcgag cgcggcctgg tactggtcgc gcatctgacc gacgttctgc
     1741 ttgtagaacg cggcgaagtc cgggtcctgc atcgggtcga tgcgtacggg tcggcccatc
     1801 ctggccgaca tctcctgctc cttggccttg agcctcgggg cgtactgctt gcggatcgca
     1861 tcctcgacgc gcttggcgtc ctctatgtac cgcgccagga agccgacgag ctgctcgaag
     1921 gtggcctgga tcttcttctc gccgaagggg gcgacgttca gggccttgag gccctcggcg
     1981 acgggggcga gttcggcttc cggctgggcc acggcgctgg tcggtagctg gatcctcgag
     2041 acgaatacgt cgaaggcgcc ctcccgcgcg gcttcgcgcc tgtccttggc tatggccgcg
     2101 aaccgggact ggaagtcgca ggccgtcggg tccgccaggt actcgccggc caggcgctta
     2161 ccttcctgct tagcctcggc ggccgccagg gccttgggat ccgccttcag gttcttggtt
     2221 cgttccatcg ctatctcgag cgcgctcttg atcatcgcca tgcgtgtctc catcgtttgt
     2281 cggtataagc ctgcccggtc cggcggccgt caccggggcg gcgcgcaggt cctgcagcat
     2341 gatgcgcagt ttatccagaa tcgagaccgc gacgacggcc ccgacgacga gttccagcgc
     2401 cgagtacgcg gccggcggca ggtcggccac gaagcgcaat agctcgaacg cgtacagggc
     2461 catggtcgaa gcgaccgtgg ccttgcccag gaacgaggtc ctcggcgtga cgcgcttctt
     2521 gacgagtagc accgccaaca ccatcagggt ctgaccgacc agcct
//
LOCUS       DAOPHV010000397         2561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_56860_length_2561_cov_4.039505, whole genome shotgun sequence.
ACCESSION   DAOPHV010000397 DAOPHV010000000
VERSION     DAOPHV010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56860_length_2561_cov_4.039505"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PLQ29_09935"
     CDS             <1..637
                     /locus_tag="PLQ29_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPG87007.1"
                     /translation="ASLAVWADALSGAHLQALWGSIRLGVWTASTSTLVGFLFAFVIH
                     RTSARGKKFLAAVAQLPVISPPFSLTLSIILLAGNNGLITKQLLGLDNFSIYGLGGLV
                     LVQTIGMFPIAYLTLAGVLQGLDATLEEAALDMNASRWQVFRTVTLPLAVPGLLSAWL
                     LVFTNSLADFANPLLLAGNYRVLSVEAYIQVTGRNDLGGGAALRYSCSYRR"
     gene            682..1518
                     /locus_tag="PLQ29_09940"
     CDS             682..1518
                     /locus_tag="PLQ29_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPG87008.1"
                     /translation="MTVTGKPNLKLVEVASPAVRIAMTAGVWLIVAFTLSLYGTIVAG
                     CFVKNWGIDYSFTLANIGEALARGKDAIRSTLTLAAVATPIAGILAMASAVIFVRKTF
                     AGKKVLEAVMMTPFAIPGTLLGIAYILAFNKAPILIVGTGAIIIINYIVRELPVGIEG
                     GVASLRQIDPSIEEAARDLGADEGMVFRSIVLPLVRPAFISSMSYTFVRSMTAVSAVI
                     FLISARWYHMTVLIYNFSENIRFGLASVLATTLIVIVMGAFGLMRLLVRDTGAFDKSI
                     SR"
     gene            1532..>2561
                     /locus_tag="PLQ29_09945"
     CDS             1532..>2561
                     /locus_tag="PLQ29_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG87009.1"
                     /translation="MAKEPVAVTVDRVTRIYKNVKGRPDVVAVDEASFVVAPGELVTL
                     LGPSGCGKTTTLRMIAGFDLPTKGQIRIGGEDVSSLPPNKRDTATVFQSYGLFPHMNV
                     AENVAYGLRIRKVPKAEIDERVKRALELTGLADLADRSPGRLSGGQQQRVALSRSLVV
                     EPGVLLLDEPLSNLDALLREQMRVEIRRIQKSLGITAVYVTHDRVEAMSLSDRVIVMK
                     DGRIRQIGAPSVIYERPDSVFVAGFVGKVAFFPVEVLGDDGEGCACSIRGKAVRAGSR
                     APGVAAGGNAMLMARPESLILGRPGEGVVDGEVRQNVYLGSSVESFVTTEYGELLVQI
                     DDPAGKRLA"
BASE COUNT          410 a          890 c          816 g          445 t
ORIGIN      
        1 ggcatcgctg gcggtctggg ccgacgcact gtccggcgcc catctgcagg cgctctgggg
       61 ctcgatacgc ctcggcgtct ggacggccag cacctccacc ctggtcggct tcctgttcgc
      121 cttcgtgatc caccgcacca gcgcccgcgg caagaagttc ctggccgcgg tcgcccagct
      181 gccggtcatc tcgccgccgt tctcgctgac tttgtcgatc atcctgctcg ccggcaacaa
      241 cggcctcatc accaagcaac tcttgggcct ggacaatttc tccatctacg gcctcggcgg
      301 cctcgtgctc gtgcagacga taggcatgtt cccgatagcc tacctgacgc tggccggcgt
      361 cctgcagggc ctcgacgcga cgctcgagga agccgcgctc gacatgaacg cgagccgttg
      421 gcaggtcttc cgcacggtca cccttccgct cgcggtaccc ggcctcctgt ccgcctggct
      481 gctcgtgttc acaaactcgc tggccgactt cgccaacccg ctcctgctcg cgggcaacta
      541 ccgcgtgctg tcggtggagg cctacatcca ggtgaccggg cgcaacgacc tcggcggcgg
      601 cgccgccctt cgctactcct gctcctaccg acgctgaccg cgttcttcat acagcgcaac
      661 tgggtctcca aacgatccta cgtgacggtc accggcaagc cgaacctcaa gctggtcgag
      721 gtcgcctcgc cggccgtgcg catcgcgatg acggccggcg tctggctcat cgtcgccttc
      781 acgctgtcgc tgtacggcac catcgtcgcc ggttgcttcg tcaagaactg gggcatcgac
      841 tacagcttca cgctggccaa catcggcgag gccctggcca ggggcaagga cgcgatacgc
      901 tcgacgctga cgctcgcggc ggtcgcgacg ccgatagcgg gtatcctggc gatggcgtcc
      961 gccgtcatct tcgtgcgcaa gaccttcgcc ggcaagaagg tcctcgaggc ggttatgatg
     1021 acgcccttcg cgatacccgg caccctgctc ggcatcgcgt atatcctggc gttcaacaag
     1081 gcgccgatcc tgatcgtcgg cactggcgcc atcatcatca tcaactacat cgtccgcgag
     1141 ttaccggtcg gcatcgaggg cggcgtagca tcgctcaggc agatcgaccc gtccatcgag
     1201 gaagccgcgc gcgatctcgg cgccgacgag ggcatggtct tcaggagcat cgtgctgccg
     1261 ctcgtcaggc cagccttcat ctcgagcatg tcgtacacct tcgtgcgctc gatgacggcc
     1321 gtcagcgcgg tgatcttcct gatctcggcg cgctggtacc acatgaccgt gctgatctac
     1381 aacttctccg agaacatcag gttcgggctc gcgagcgtgc tcgcgacgac cctgatcgtc
     1441 atcgtcatgg gcgccttcgg cctcatgcgg ctcctcgtgc gcgacacggg tgccttcgat
     1501 aaatccataa gcagataggc gggagcgaac catggctaag gaaccggtag cggtcacggt
     1561 agaccgcgta acgagaatat acaagaacgt gaagggtcgc cccgacgtgg tggcggtcga
     1621 cgaggcgagc ttcgtcgtcg ccccgggcga gctcgtgacc ctgctcgggc cgtccggctg
     1681 cggcaagacg acgacgctca ggatgatagc gggcttcgac ctgccgacga agggtcagat
     1741 acgcatcggc ggcgaggacg tcagctcgct gccgccgaac aagcgcgaca cggcgacggt
     1801 gttccagagc tacggcctgt tcccgcacat gaacgtcgcg gagaacgtcg cctacggcct
     1861 caggatacgc aaggttccca aggccgagat cgacgaacgg gtcaagcggg cgctggagct
     1921 gacagggttg gccgatctgg cggaccgatc gccgggcagg ctatccggcg gccaacagca
     1981 gcgcgtcgcc ctgtcgcgca gcctcgtcgt cgagcccggc gtgctcctgc tcgacgagcc
     2041 gctgtccaac ctggacgccc tcctgcgcga gcagatgcgc gtcgagatcc gccgtatcca
     2101 gaagtccctc ggcatcacgg cggtctacgt gacccacgac cgcgtcgagg cgatgagcct
     2161 gtccgatcgc gtcatcgtga tgaaggacgg ccgcatccgg cagatcggag ccccgagcgt
     2221 catctacgag cggccggatt cggtcttcgt cgccggcttc gtcggcaagg tggccttctt
     2281 cccggtcgag gtgctcggcg acgacggcga aggttgcgcc tgctcgatac gcggcaaggc
     2341 ggtccgagcc ggctcgcggg cgcccggcgt tgcggcgggc ggtaacgcca tgctgatggc
     2401 ccgccccgag tccctcatcc tcggccgccc cggcgagggc gtcgtcgacg gcgaggtcag
     2461 gcagaacgtc tacctcggct cgagcgtcga gtcgttcgtg accaccgagt acggcgagct
     2521 gctcgtgcag atcgacgacc cggccggcaa gcgcctcgcg g
//
LOCUS       DAOPHV010000398         2556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_57018_length_2556_cov_4.331068, whole genome shotgun sequence.
ACCESSION   DAOPHV010000398 DAOPHV010000000
VERSION     DAOPHV010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57018_length_2556_cov_4.331068"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLQ29_09950"
     CDS             <1..216
                     /locus_tag="PLQ29_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87010.1"
                     /translation="DVLVYYDGERIVNLNALFKWGKVKHGSVIQFAVAGENICDVAKL
                     QRYLAQGASPMFEAFLRGPVNGVLALF"
     gene            230..>2556
                     /locus_tag="PLQ29_09955"
     CDS             230..>2556
                     /locus_tag="PLQ29_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HPG87011.1"
                     /translation="MKANVHFFSQKESIAKGIDLETLGQRGRQANEFSELGFPILPGF
                     IVDSGVASRLAEEDVVTAVKPALRKVSAIVNKAYGDDAEPLLLKIVISPNLAISNYPT
                     LHNFGLVRSSVQGFNRNVGENFGAHEVAFLARGMLKIEERIAELEGRDKDVAKIRERI
                     VAASKTLDSDKPGAKAEALMASYEDLLPAGFFDDAWTQLELALKRISKMLDLDDQDDD
                     DTAILVQPMVYGNYGKGSCSGSFFTRNVVNGDKTLQGEFFEEHFDELGATGNDINKIA
                     PVHLKELRRIAASLEDHHREIRQIRFTIEKNNLWLIEQRQVVAKSTQADLKLYLDLNS
                     RKVVDDAFVINSIQPGRLNEILHPIVDLSSVKKLPFTSGGITGAPGAAIGRVYFTTET
                     LIEAYRVAQQKGEDKRFILVMPSTFAEDVKAIEVATGVLSCEGGYSAHASVVARQYGK
                     VSLVKPEMRIRGKKATIGELAVSEGDYITINVPYYGEPTIWLGKASLIEPDPAESGLF
                     EFIAIVKKHMKHFGVRANADSPRDAALALSFGADGIGLCRTEHMFFNDKRINVFREMI
                     LADSVEAREKALGKLEVMQRDDFYGILKTMAGKDVTIRLLDAPLHEFMPHNDAEFESF
                     MAYLGSGKKGYKAPSKAEIRARIEALAEFNPMLGHRGCRIAVSYPEIYAMQTRAIFQA
                     AYKLRDEKVDVRPEIMIPIVMNASELKLIVYGKKIEGKAYKGIVEIEEDARKVLKAKP
                     IEYHVGTMIELPAAALTAGEIAKYSEFFSFGTNDLT"
BASE COUNT          546 a          786 c          785 g          439 t
ORIGIN      
        1 gacgtgctcg tctactacga cggcgagcgc atcgtcaacc tgaacgcgct gttcaagtgg
       61 ggcaaggtca agcacggtag cgttatccag ttcgcggtcg ccggtgagaa catctgcgac
      121 gtggcgaagc tgcaacgcta cttggcgcag ggcgcgagcc cgatgttcga ggccttccta
      181 cgcggtccgg tcaacggcgt cctggcgctc ttttgagtaa ggagaaacca tgaaagccaa
      241 tgtgcacttc ttcagccaga aggaatccat agccaaaggc atcgacctcg agacgctcgg
      301 tcagcgcggc aggcaggcga acgaattctc cgagctcggt tttcccatac tacctggctt
      361 catcgtcgac tcgggagtcg cctcgcggct ggccgaggag gacgtcgtca cggcggtcaa
      421 gccggccctg cgcaaggtat cggccatagt caacaaggcc tatggcgacg atgccgaacc
      481 gctcctcctc aagattgtca tctcgccgaa cctcgccatc tcgaactacc cgacgctcca
      541 caactttggg ctcgtgcgtt cgtcggtcca gggcttcaac cgcaacgtcg gcgagaactt
      601 cggcgcccac gaggtggcct tcctggccag gggaatgctc aagatagagg agcgcatcgc
      661 cgagctcgag gggcgcgaca aggacgtggc caagatccgc gagcgcatcg tggcggccag
      721 caagaccctg gactccgata agcccggggc caaggccgag gcgctgatgg cctcctacga
      781 ggatctgcta ccggctggtt tcttcgacga cgcctggact cagctcgaac tggccttgaa
      841 gcgcatctcc aagatgctcg acctggacga ccaggacgac gacgatacgg ccatactcgt
      901 ccagccaatg gtctacggca attacggtaa aggctcgtgc tccggttcgt tcttcacgag
      961 gaacgtggtc aacggcgaca agacgctaca gggcgagttc ttcgaggagc actttgacga
     1021 gcttggcgcc accggcaacg atatcaataa gatagccccg gtacacctca aagagctcag
     1081 gcgtatagcc gcctcgctcg aggatcatca cagggagatc cggcagatac gcttcacgat
     1141 cgagaagaac aacctgtggc tgatcgagca acggcaggtt gtcgcgaaga gcacccaggc
     1201 cgacctgaag ctatacctcg acctgaactc gcgcaaggtc gtcgatgacg ccttcgtgat
     1261 caactcgatc cagccggggc gtcttaacga gatactccat cctatcgtcg acctgagctc
     1321 ggtcaagaag ctgccgttca catccggcgg catcaccggc gccccgggcg cagcgatagg
     1381 ccgcgtctac ttcacgaccg agacgctcat cgaggcctac agggttgccc aacagaaggg
     1441 cgaggataag cgattcatcc tggtcatgcc gtcgaccttc gccgaggacg tcaaggccat
     1501 cgaggtcgcg acgggcgtgc tgtcctgcga aggcggctac tcggcgcacg cctcggtcgt
     1561 ggcgcgccag tacggcaagg tctccctcgt caagccggag atgaggattc gcggcaagaa
     1621 ggcgacgata ggcgagctgg cggtttccga aggcgactat atcaccatca atgtgccgta
     1681 ctacggcgag ccgacgatct ggctcggtaa ggcgtcgctg atcgagccgg atcccgccga
     1741 gtcgggcctg ttcgagttca tcgccatcgt caagaagcac atgaagcact tcggcgttcg
     1801 cgccaatgcc gacagcccga gggacgccgc cctggccctg tccttcggcg ccgacggcat
     1861 tggcctctgc cgcaccgagc acatgttctt caacgataag cgcatcaacg tcttcaggga
     1921 gatgatactc gcggattcgg tggaggctcg cgagaaggct ctcggtaagc tcgaggtgat
     1981 gcagcgcgac gatttctatg ggatcctcaa gacgatggcc ggcaaggacg ttacgatccg
     2041 gctcctcgac gccccgctcc atgaattcat gccgcacaac gacgccgagt tcgagagctt
     2101 catggcctac ctcggctccg gcaagaaggg gtacaaggcg ccgtctaagg cggagatccg
     2161 ggcgcgcatc gaggcgctgg ccgaattcaa cccgatgctc ggccaccgcg gctgccgcat
     2221 cgcggtcagc taccccgaga tctacgcgat gcagacgcgg gccatcttcc aggccgccta
     2281 taagctgcgt gacgagaagg tggacgtgcg accggagatc atgataccga tcgtgatgaa
     2341 cgcctcggag ctaaagctga tcgtctacgg taagaagatc gaaggcaagg cctacaaggg
     2401 catcgtcgag atcgaggagg acgcgcgcaa ggtgctgaag gccaagccaa tagagtacca
     2461 cgtcgggacg atgatagagc ttcccgcggc ggcgctcacc gccggggaga tcgccaagta
     2521 cagcgagttc ttcagcttcg gcacgaacga cctgac
//
LOCUS       DAOPHV010000399         2553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_57114_length_2553_cov_3.863090, whole genome shotgun sequence.
ACCESSION   DAOPHV010000399 DAOPHV010000000
VERSION     DAOPHV010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57114_length_2553_cov_3.863090"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PLQ29_09960"
     CDS             complement(<1..849)
                     /locus_tag="PLQ29_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPG87012.1"
                     /translation="MPSLIDGYLAYLGAIRSLSPRTVAAYGNDLRLFSALVEGAGRSV
                     EAAEPADVRDFIASLVRDGYMSSSVNRALSAVKGFYRYLLRYGHASTNPAKDVESLPM
                     ARTLPAFMFEDEMAEFIDGSPDDGFAGARDRAIFETLYSTGCRVSELAGLTPADVDLR
                     AGRARVTGKGSKERVVFLSDTARAAIEAWLPYRSARLAGMGEDEARHFGLFLNAKSGP
                     LSTRGIAYIVEWRMVASGRSRRLSPHGFRHSFATHLVGRGADIRVVQALLGHRNISTT
                     QVYTHVD"
     gene            complement(833..>2553)
                     /gene="topA"
                     /locus_tag="PLQ29_09965"
     CDS             complement(833..>2553)
                     /gene="topA"
                     /locus_tag="PLQ29_09965"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPG87013.1"
                     /translation="SALDEDKVNAQKARRVLDRLVGYNLSPLLWKKVKNGLSAGRVQS
                     VALRLICEREVEVESFIPEEYWSLEADFKKGRYAFSGELVSYDGAKAELKGESDVEAI
                     TRAIEGLPCLVSDVKESERQVKPKPPFTTSKLQQTAANRLGFTSKKTMQIAQQLYEGI
                     NIGSTRIGLITYMRTDSVRVSDVALKEVRDWLGERFPAELPAEPVVYATDKKAQDAHE
                     AIRPTMVAYTPDAIKEYLNKDQQKLYGIIWERFVASQMTQAKQRSTSMEVSVGLARFR
                     VGASRLVEKGFYKVIKLLATKDDKESTLPEMRIGEELKLEGYRPEQHFTQGPARFTDA
                     SIIKLLEEKGIGRPSTYAPIISVLLERYYVTRENKQLVPTTLGRKINEILVGSFGDVV
                     DAGFTADMENMLDQVEESKRDWVSAMGEFYGPFKKRIDDIMATLQSFKGSLDEPTDQV
                     CEKCGRPMVKKLGRFGFFLACTGFPDCKNTKSIPLARCPRPGCTGEIVARRKSKGRGR
                     EFYGCSRYPECDFITYYKPTNSYCPKCGWFLVERFDKKRGSYKACINPACDYLHSQDE
                     DHAVPD"
BASE COUNT          417 a          825 c          840 g          471 t
ORIGIN      
        1 gtccacgtgc gtatacacct gcgtggtcga aatgttccga tgtccgagca gcgcctgtac
       61 caccctgatg tcggccccgc ggccgacgag gtgggtggcg aagctatgcc tgaagccgtg
      121 aggcgacagc cgccggctgc gccccgacgc aaccatccgc cactcgacga tgtacgctat
      181 gccacgggtc gaaagaggcc cggacttggc gttcaggaag aggccgaagt gcctggcctc
      241 gtcctcgccc atgccggcta gcctggccga ccggtacggg agccaagcct cgatagccgc
      301 cctcgccgta tcggacagga ataccacacg ctccttcgag cccttgccgg tcaccctggc
      361 ccgaccggcc cgtaagtcga cgtcggccgg ggtcaggccc gcaagctccg acacgcggca
      421 gccggtgctg tacagcgtct cgaagatggc ccggtctcgg gcgcccgcga agccgtcatc
      481 gggcgagccg tcgataaact cggccatctc gtcctcgaac atgaaggcag gcagcgtcct
      541 ggccatcggc agggactcga cgtccttggc cgggttcgtc gaggcgtggc cgtagcggag
      601 gaggtagcgg tagaagccct tgaccgccga cagggcgcga ttcacggagc tgctcatgta
      661 gccgtcgcgg acgagcgagg cgatgaagtc ccggacgtcg gcgggctcgg cggcctcgac
      721 ggagcggccg gcgccctcga ccaacgccga gaacaggcgc aggtcgttgc cgtaggccgc
      781 gacggtacga ggcgacaggg agcgtatcgc gccgaggtag gccaggtagc cgtcaatcag
      841 ggacggcatg gtcttcgtcc tggctgtgca ggtagtcgca ggccgggttg atgcaggcct
      901 tatacgaacc tcgcttcttg tcgaagcgct ccacgaggaa ccacccgcac ttggggcagt
      961 agctgttggt cggcttgtag tacgtgatga agtcgcactc cgggtaacgc gagcagccgt
     1021 aaaattcgcg gccgcgcccc ttggatttac gcctggccac gatctcgccg gtgcagccgg
     1081 gcctggggca gcgcgcgagc gggatgctct tcgtgttctt gcaatccggg aagcccgtgc
     1141 aggccaggaa gaagccgaag cggccgagct tcttgaccat cggccgcccg catttctcgc
     1201 agacctggtc cgttggctcg tcgaggctac ccttgaacga ctggagcgtg gccatgatgt
     1261 cgtcgatgcg cttcttgaac gggccgtaga actcgcccat cgcggagacc cagtcgcgct
     1321 tcgactcctc gacctggtcg agcatgttct ccatgtcggc cgtgaagccg gcgtcgacca
     1381 cgtctccgaa cgagccgacg agtatctcgt ttatcttacg gcccagggtg gtcggcacca
     1441 gctgcttgtt ctcccgggtc acatagtacc gttcgagcag caccgatatg atcggcgcat
     1501 aggtggacgg gcggccgatg cccttctcct cgagcaactt gatgatcgaa gcatcggtaa
     1561 agcgggccgg cccctgcgtg aagtgctgct ccggccgata gccctcgagc ttgagctcct
     1621 cgccgatacg catctccggc agggtcgact ccttgtcgtc cttcgtcgcg agcagcttta
     1681 tgaccttgta gaagcccttc tccactagcc gcgaggcgcc gacgcggaac ctggccaggc
     1741 cgacggagac ttccatcgag gtcgagcgct gcttggcctg ggtcatctgg ctggcgacga
     1801 agcgctccca gattatgccg tacagcttct gctggtcctt gttcaggtac tccttgatcg
     1861 catccggcgt ataggcgacc atcgtcggcc ggatcgcctc gtgcgcgtcc tgggccttct
     1921 tatcggtggc gtagacgacg ggctcggcgg gcagctcggc ggggaaacgc tcgccgagcc
     1981 aatcgcgcac ctccttgagc gccacgtccg agacgcgcac cgagtcggta cgcatatagg
     2041 taatgaggcc gatgcgggtc gagccgatgt tgatgccttc gtacagctgc tgggctatct
     2101 gcatcgtctt cttgctggtg aagccgagcc tattggccgc ggtctgctgc agcttggagg
     2161 tcgtgaaggg cggcttcggc ttgacctggc gctcgctttc ctttacgtcg gagaccaggc
     2221 agggcagccc ttcgatggcg cgagtgatgg cctcgacgtc ggactcgccc ttgagctcgg
     2281 ccttcgctcc gtcgtatgag acgagctcgc ccgagaacgc gtagcgcccc ttcttgaagt
     2341 ccgcctcgag cgaccagtac tcctcgggga tgaacgactc cacctcgacc tcgcgctcgc
     2401 agatcaggcg cagggcgacc gactggacgc ggccggccga gaggccgttc ttgaccttct
     2461 tccagagtag cggggacagg ttgtagccga cgagacggtc gagcacgcgc ctggccttct
     2521 gggcgttcac cttgtcctcg tcgagggccg acg
//
LOCUS       DAOPHV010000400         2550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_57197_length_2550_cov_3.723447, whole genome shotgun sequence.
ACCESSION   DAOPHV010000400 DAOPHV010000000
VERSION     DAOPHV010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57197_length_2550_cov_3.723447"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PLQ29_09970"
     CDS             <1..595
                     /locus_tag="PLQ29_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87014.1"
                     /translation="LLAGEPADYPIHGSQVRKTMSNPFSYSDRDLAVFDMDRCLIVDS
                     DRDYEDVLLICELANYQYLELRALDKWLDKWLDEAEDDVNLIARKGAGRGLKTGTLRK
                     KLGKIQALRLDALFILENLENSSKIIGDYFLGQVYEHVCGIFNTSGWTRSVERRLDAL
                     QDIYEIVKTENSERTMLLLEIVFIVVCIIPIIQFAFG"
     gene            678..2018
                     /locus_tag="PLQ29_09975"
     CDS             678..2018
                     /locus_tag="PLQ29_09975"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPG87015.1"
                     /translation="MVILTLNCGSSSVKYQVYDWDEKNVLASGIVERVGIEGSIINHK
                     APGVADVSLEHECPNHVVAVELILKTITDPQHGVVRSMKDIGAVGHRMVHGGMKFARS
                     VLITDESLATFKELIDLAPLHNPANITGVEAAKAVLPNVPHCAVMDTAWHQTMPSTSY
                     TYALPHEWQDKYMVRRYGFHGTSFLYTAKRASVLLGKDPFDSNVIIAHIGNGSSINAV
                     KDGCSYDTSMGMTPLEGLVMGTRSGDIDPGIVFHMMKKAGLSAAEVEKALNKESGVLG
                     ITGRWSDRRDIENAAAEGDRIAIVAQELEGYRIKKYIGAYYAALGHVDALVFTAGVGE
                     MGPVTRHLSTLGLEGMGIKVDPARNEKAKCRNAELEISAPDSPVKVYVIPTDEELVMT
                     EDAFALMNGTYDVHTNYTYSFQSPDYVNKQRAAGLEKDLAKKPYLADLIAHPRR"
     gene            2022..>2550
                     /locus_tag="PLQ29_09980"
     CDS             2022..>2550
                     /locus_tag="PLQ29_09980"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HPG87016.1"
                     /translation="MATSLRAACMRRVIRLIFKEQGLSVERERERSLATGKAHPRFPR
                     SVRVGRFDIDGMPCASIEPASAGRVGPVRVVLYLHGGGYVTGFIDSSLMLCVPMAREL
                     GLRLVLPEYRLAPEHPFPAAIDDALKAYRRLLSEGYAPGEIALAGDSAGGGLCLAVLQ
                     AIRDLGGPVPAAALDD"
BASE COUNT          482 a          885 c          766 g          417 t
ORIGIN      
        1 gctcctcgcc ggcgagccag ccgattatcc catccacggg tcgcaggtcc gcaagacgat
       61 gagcaacccg ttctcctaca gcgaccgaga cctggcagtc ttcgatatgg accgatgcct
      121 catcgtcgac tcggaccgcg actacgagga cgtgctgctg atctgcgagc tggccaacta
      181 ccagtacctc gagctgcgcg ccctggataa gtggctcgat aaatggctcg acgaggccga
      241 ggacgacgtc aacctgatag ccaggaaggg agcgggtcgc gggctcaaga ccggtaccct
      301 gcgcaagaag ctcggcaaga tccaggctct gcgcctcgac gcgctgttca tcctcgagaa
      361 cctcgagaac agctcgaaga tcattggcga ctatttcctg ggccaggtct acgagcatgt
      421 ctgcggcatc ttcaacacct cggggtggac ccgctcggtc gagcggcgcc tcgacgcgct
      481 ccaggatatc tacgagatcg tcaagaccga gaactcggaa cgcaccatgc tgctgctcga
      541 gatcgtgttc atcgtcgtct gtatcattcc catcatccag ttcgcgttcg gctagccagc
      601 gtccgtcccg cgctctgtag acccgcgctc gcgtctctgg tacagtctct cctgataacc
      661 acgctaagga gaacctcatg gtcatactta ccctcaactg cggcagttcg tccgtcaagt
      721 accaggtcta cgactgggac gagaagaacg tactcgcgag cggcatcgtc gagcgcgtcg
      781 gcatcgaagg ctcgatcatc aaccacaagg cgccaggcgt cgccgacgtg agcctcgagc
      841 acgagtgccc caaccacgtc gtcgcagtcg agctgatact caagaccatc accgacccgc
      901 agcacggcgt cgtacggtcg atgaaggaca tcggcgcggt cggccaccgc atggtccacg
      961 gcggcatgaa gttcgcccgc tctgtgctga tcaccgatga gtccctggct accttcaagg
     1021 aactcatcga cctggcaccg ctccataatc ccgccaacat caccggcgtc gaggcggcca
     1081 aggccgtgct tccgaacgtg ccccattgcg ccgtcatgga caccgcctgg caccagacca
     1141 tgcccagcac cagctacacc tacgcattgc cccacgaatg gcaggacaag tacatggtgc
     1201 ggcgctacgg cttccacggc accagcttcc tctatacggc caagcgcgcc tcggtgctgc
     1261 tcggcaagga tcccttcgac tcgaacgtca tcatcgcgca catcggcaac ggctcatcga
     1321 tcaacgccgt caaggacggc tgctcctacg acacctcgat gggcatgacc ccgctcgagg
     1381 gcctggtcat gggcacccgc tccggcgata tcgaccccgg catcgtcttc cacatgatga
     1441 agaaggccgg cctgtccgca gccgaggtcg agaaggcgct gaataaggaa tccggcgtgc
     1501 tcggcatcac cggccgctgg tccgacaggc gcgacatcga gaacgccgcc gccgagggcg
     1561 ataggatcgc catcgtcgcc caggagctcg agggctaccg catcaagaag tacatcggcg
     1621 cctactacgc cgcgctcggc cacgtcgacg ccctcgtgtt taccgctggc gtcggcgaga
     1681 tgggtccggt cacccgccac ctgtcgaccc tcggcctcga gggtatgggt atcaaggtcg
     1741 accccgcccg caacgagaag gccaagtgcc gtaacgccga gctggagatc agcgcccccg
     1801 acagcccggt caaggtctac gtgatcccga ccgacgagga gctcgtgatg accgaggacg
     1861 ccttcgcgct gatgaacggc acctacgacg tccacaccaa ctatacgtac agcttccaga
     1921 gccccgacta cgtcaacaag cagcgagccg ccgggctgga gaaggacctc gccaagaagc
     1981 cgtacctggc ggacctgata gcgcatccga ggcgctaaac catggcaacg agccttcgcg
     2041 ccgcctgcat gcgccgcgtg atccgcctga tattcaagga gcagggtctc agcgtcgagc
     2101 gggagcgcga acgctcgctg gccacgggta aggcgcaccc tcggtttccg cgctcggtgc
     2161 gcgtaggccg attcgatatc gacggcatgc cctgcgcctc gatcgagccg gccagcgccg
     2221 ggcgggtcgg cccggtccga gtcgtgctct acctgcacgg cggcggctac gtgaccggct
     2281 tcatcgactc gtcgctgatg ctctgcgtgc cgatggcgcg ggagctcggc ctgcgcctgg
     2341 tgcttccaga gtaccgcctc gcgcccgagc atcccttccc cgcggcgatc gacgacgcgc
     2401 tcaaggccta ccgccgactt ctgtccgaag gctacgcgcc cggcgagata gcgctggccg
     2461 gggactcggc gggaggcggg ctgtgcctgg ccgtgctcca ggccatccgc gacttgggcg
     2521 ggcccgtgcc cgccgcggcc ctggatgacg
//
LOCUS       DAOPHV010000401         2540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_57487_length_2540_cov_3.986318, whole genome shotgun sequence.
ACCESSION   DAOPHV010000401 DAOPHV010000000
VERSION     DAOPHV010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57487_length_2540_cov_3.986318"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLQ29_09985"
     CDS             <1..229
                     /locus_tag="PLQ29_09985"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPG87017.1"
                     /translation="FICAEGDVIEADSIAPEDHGRHGATKGLHELVGEFEREVILRRL
                     AFFKGNRTATAQDLGISLRNLQYKLEKYDLR"
     gene            399..1730
                     /locus_tag="PLQ29_09990"
     CDS             399..1730
                     /locus_tag="PLQ29_09990"
                     /inference="COORDINATES: protein motif:HMM:NF014697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HPG87018.1"
                     /translation="MIRALGNFFTKIAKRWIADAFVFAIVLTIIVYACGMLIEKASFL
                     DMATYWGKGFWGLLSFSMQMVAVLVTGSALAISKPVHRAMVSIANVANTPAKAIIIVT
                     VVALVGNWFNWGFGLIVSAILAKEIAKVSKGIHYPLLVASAYSGFVIWHSGLSASIPL
                     SIAGKTPVMEKFAHGAIVPTSETIFSFQTLTIVIVILISLPILNMMMLPKKKEDIIEV
                     DPALFADDEEEIPVKARKDMTPAEKQENSLILALFVVVIGAFYIVRFFVKGGSLNLDI
                     VNIIFLILGLAMHRTPIAYVHAIDKSIRNCGGIVLQFPFYGGLMGMMVDSGLAATLAN
                     AFVAISTPKTFQLFTYYAGGIINMFVPSGGGQWAVQGPIMMPAAEALGVSYGRTAMGI
                     AWGDAWTNMIQPFWALPLLGVAKLSIKDIMGYTTVVLLWVGVVTSLGLLFL"
     gene            1859..2536
                     /locus_tag="PLQ29_09995"
     CDS             1859..2536
                     /locus_tag="PLQ29_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HPG87019.1"
                     /translation="MVQKPIVTADEAVKLIKEGDAVMVGGFLGCGSPTGLIAALEKRG
                     TGKMTLICNDCAMYNPKTGEMNGVAPLVAKKRFSTAVISHIGTNPEIQRQMNEGETDV
                     QLVPQGTLAERIRAAGAGLGGVLTPTGLGTEVQDGKKVVEVDGAKFLLEKALSGDVAL
                     IRAKKADKSGNCVYDKSGRNFNPLMAMACGTVIVEADEIVEIGELDPEVVVTPSVFVT
                     HLVQGRK"
BASE COUNT          483 a          810 c          756 g          491 t
ORIGIN      
        1 gtttatctgc gccgagggcg acgtgatcga ggccgactcc atcgcccccg aggaccacgg
       61 ccgccatggc gctacgaagg gcctgcacga gctcgtcggc gagttcgagc gcgaggtgat
      121 cctccgccgg ctggccttct tcaagggcaa ccggacggca actgcccagg acctgggcat
      181 atcgctacgg aacctgcagt acaagctcga gaagtacgac ctgcgctgaa cccgttcctc
      241 gtacggccac gcaagaattt gcgtggtgtc gcgccagaac ttgcgcgatc catgtcgtcg
      301 tatccgtcct cggctctaaa tagggctgtt tcaggctctg catcgagctg gcacggttgt
      361 tgctctgata actcctagaa agtttctagg aggcatctat gatacgagct ctcggcaatt
      421 tctttaccaa gatcgccaag cgctggatag cggacgcgtt cgtgttcgct atcgtactga
      481 cgatcatcgt ctacgcctgc ggtatgctca tcgagaaggc gtcgttcctc gacatggcga
      541 cgtactgggg caagggtttc tggggcttgc tcagcttctc gatgcaaatg gtcgctgtac
      601 tggtaaccgg tagcgccctg gcgatatcga aaccggtgca cagggctatg gtctctatcg
      661 ccaatgtggc taataccccc gcgaaggcta tcataatcgt caccgtcgtg gcgctcgtgg
      721 gcaactggtt caactggggc tttggcctga tcgtctccgc tatcctggcc aaggagatag
      781 cgaaggtcag caagggcatc cactacccgc tgctcgtagc cagcgcctat tccggcttcg
      841 tcatctggca ctccggacta tcggcttcca tcccgttgtc gatcgccggc aagacccccg
      901 tcatggagaa attcgcgcac ggcgccatcg tgccgacgag cgagaccatc ttctccttcc
      961 agaccctcac gatcgtcatc gtcatactca tatcgctgcc gatactcaac atgatgatgc
     1021 tgcccaagaa gaaggaagac atcatcgagg tcgatcccgc actcttcgcg gatgacgaag
     1081 aggagattcc agtcaaggcg cgcaaggaca tgacgccggc cgagaagcag gagaattcgc
     1141 tcatcctcgc actcttcgta gtggttatag gcgccttcta catcgtgcgc ttcttcgtca
     1201 agggcggctc gctgaacctc gacattgtca acatcatctt cttgatattg ggactggcca
     1261 tgcacagaac gccgatcgcg tatgtgcacg cgatcgacaa gtcgatcagg aactgcggcg
     1321 gcatcgtgct ccagttcccg ttctatggcg gactgatggg catgatggtc gattccgggc
     1381 tcgccgccac cctggccaac gccttcgtcg ccatctcgac gccgaagacc ttccagctgt
     1441 tcacctacta cgctggcggc atcatcaaca tgttcgtgcc gtccggcggc ggccagtggg
     1501 ccgtccaggg cccgatcatg atgccggccg ccgaggccct cggcgtgtcg tatggccgca
     1561 ccgcgatggg catagcctgg ggcgatgcgt ggaccaatat gatccagccg ttctgggccc
     1621 tgcctctgct cggcgtagcc aagctcagca tcaaggacat catgggctat acgaccgtcg
     1681 tcctcctgtg ggtgggcgtg gtcacctccc tcggattgct tttcctctaa gactgaccgc
     1741 ttaacggtcg ggcgtcgccg atcggcttcg cccgacctct cttccggcct tcctccagac
     1801 acacgcctcg ctacgcgcgc ctgggacggg ctcattccga tcgataagga gcgacgatat
     1861 ggtacagaaa cccatcgtca cggccgacga ggccgtgaaa ctcatcaagg agggagacgc
     1921 cgtcatggtc ggcggcttcc tcggctgcgg ctcgccgacc ggcctgatag cggcgctcga
     1981 gaagcgcggc accggcaaga tgaccctgat ctgcaacgac tgcgccatgt acaaccccaa
     2041 gaccggcgag atgaacggcg tggccccgct cgtggccaag aagcgcttca gcacggccgt
     2101 catctcgcac atcggcacca accccgagat ccagcgacag atgaacgagg gcgagaccga
     2161 cgtccagctc gtgccccagg gcactctcgc cgagcggatc cgcgcggccg gcgccggcct
     2221 cggcggcgtg ctgacgccga ccggcctcgg caccgaggtc caggacggca agaaggtcgt
     2281 cgaggtcgac ggcgcgaagt tcctgctcga gaaggccctc tccggcgacg tggcgctcat
     2341 ccgcgccaag aaggccgaca agtcaggcaa ctgtgtgtac gacaagagcg gccgcaattt
     2401 caacccgctg atggccatgg cctgcgggac ggtcatcgtc gaggcggacg agatcgtgga
     2461 gatcggtgag ctcgacccgg aggtcgtcgt caccccgtcg gtcttcgtca cgcacctcgt
     2521 ccagggcagg aagtaggagt
//
LOCUS       DAOPHV010000402         2518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_58174_length_2518_cov_4.233455, whole genome shotgun sequence.
ACCESSION   DAOPHV010000402 DAOPHV010000000
VERSION     DAOPHV010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58174_length_2518_cov_4.233455"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /gene="hflK"
                     /locus_tag="PLQ29_10000"
     CDS             <1..735
                     /gene="hflK"
                     /locus_tag="PLQ29_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HPG87020.1"
                     /translation="TEQFGFRTAQLGVQARYEDRTYPEESTMLTGDLNIVDVEWIIQY
                     RITDAKAWLFNLQDKQKTIRDISQSAINMLVGDMTILDVMGSQRVAIEQAGQQLMNET
                     LKGYGLGIEIIAVKLQNIVPPVGVQAAFEDVNKAIQDMNRLISEGKEAYNKEIPKARG
                     EADRIVQIAKGYAAERVNKANGDVARFSSVYEEYRKAPDVTRDRLYYEMIEAVFAEER
                     NVELIDSELSNFIPIKNFGTQSGGTK"
     gene            732..1712
                     /gene="hflC"
                     /locus_tag="PLQ29_10005"
     CDS             732..1712
                     /gene="hflC"
                     /locus_tag="PLQ29_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HPG87021.1"
                     /translation="MKKLITVLVVAVVLLGLLVLMGPFYIQQEGFQSIIVRFGQITRV
                     ETEAGLKLKTPFIDNVVTYPKKILMWDGEAQRIPTKENQFIWVDATARWRISDPAKFY
                     ESISTLDSAFGKLDDVIDSAVRTVISSNFLREAVRNSNDIMHSTATETFETGDAEGSD
                     SLRQLTQTDASYDTIDKGRRALSGDMLAMAQQLTPEYGIEIIDVIPRQIRYSDELTES
                     VYNRMIKERNQIAQAFRSFGEGKKSEWMGKLENEQRSILSKAYEEAEVIKGTADARAT
                     KIYADAYGRDRSFFDFWRAIESYRATMPKFTKTLSTDMSYFDYLYGPKGR"
     gene            complement(1965..2518)
                     /locus_tag="PLQ29_10010"
                     /pseudo
     CDS             complement(1965..2518)
                     /locus_tag="PLQ29_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739781.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
BASE COUNT          555 a          808 c          749 g          406 t
ORIGIN      
        1 accgaacagt tcggcttccg cacggcccag ctgggcgtcc aggcgaggta cgaggaccgg
       61 acctaccccg aggaatccac catgctgact ggcgacctca acatcgtcga cgtcgagtgg
      121 atcatccagt accgcatcac cgacgccaag gcctggctgt tcaacctcca ggacaagcag
      181 aagaccatac gcgacatcag ccagtcggcc atcaacatgc tcgtcggcga catgaccatc
      241 ctcgacgtga tgggatcgca gcgcgtggcc atcgagcagg ccggccaaca actgatgaac
      301 gagaccctga agggctacgg cttgggcatc gagatcatag ccgtcaagct ccagaacatc
      361 gtgccgccgg tcggcgtgca ggccgcgttc gaggacgtca acaaggccat ccaggacatg
      421 aaccgcctga tctccgaagg caaggaagcc tataacaagg agatcccgaa ggcccgaggc
      481 gaggccgacc gcatcgtcca gatagccaaa ggctacgcgg ctgagcgcgt caacaaggcc
      541 aacggcgacg tggcccgctt cagctccgtc tacgaggagt accggaaggc gcccgatgtg
      601 actcgggatc gtctgtacta cgagatgatc gaggcggtgt tcgccgaaga gcggaacgtc
      661 gagctgatcg acagtgagct cagcaacttc ataccgataa agaacttcgg tacccagtcc
      721 ggaggcacca agtgaaaaaa ctcataaccg tcctggtcgt ggccgtcgtc ctgctcggcc
      781 tcctcgtctt gatgggcccc ttctatatcc agcaggaggg ctttcagtcg atcatcgtcc
      841 gcttcggcca gatcaccagg gtcgagaccg aggccggcct caagctcaag actccgttca
      901 tcgacaacgt ggtcacctac cccaagaaga tcctcatgtg ggacggcgag gcccagcgta
      961 tcccgaccaa ggagaaccag ttcatctggg tggacgccac cgcgcgctgg cgcatctccg
     1021 acccggccaa gttctacgag tccatctcca cgctcgacag cgccttcggc aaactcgacg
     1081 acgtgatcga cagcgccgta cgcaccgtca tctcgtcgaa cttcctgcgc gaggccgtgc
     1141 gcaacagcaa cgacatcatg cactccaccg ccacggagac cttcgagacc ggcgacgccg
     1201 agggctccga ctcgctcagg cagctgaccc agaccgacgc gtcctatgac accatcgaca
     1261 agggacgccg cgcgctatcc ggggacatgc ttgcgatggc ccagcagctg acgcccgagt
     1321 acggcatcga gatcatcgac gtgataccgc gccagatccg ctactccgac gagctaaccg
     1381 agagcgtcta caatagaatg atcaaggaac gcaaccagat cgcccaggcc ttccgctcct
     1441 tcggcgaggg caagaagtcc gagtggatgg gcaagctcga gaacgaacag cgttcaatac
     1501 tttccaaggc ctacgaggaa gcggaggtga tcaagggaac cgccgacgct cgggctacca
     1561 agatctacgc cgacgcctac ggccgcgacc ggagtttctt cgacttctgg cgcgccatcg
     1621 agtcgtaccg ggccacgatg cccaaattca cgaaaaccct gtcgaccgac atgagctact
     1681 tcgactacct ctacgggcct aagggccgat agccgaagtt cgatacgcac gaagcccgcc
     1741 ccgtcagagg cgggcttttt tatgctaggg ccccatcatc tatgcggtcg cagctcgggg
     1801 actcggcgtt catcccggta tagtagctcg atcggcggca tctgcgccat gaggaaggtc
     1861 atcgtcttgc cgggtgaacg ggatcaacct ctggtcccgg tagaataaaa accggccgcc
     1921 gagcgtagcg cccgacggcc ggctgaacgg agacgagaga cgctctagcc caggaacgac
     1981 agcatgacgc ctgccgctac cgcgcttccg atgacgccag ccacgttcgg gcccatggcg
     2041 tgcatgagga ggtagttgtc ggggttctcg cgctgcccac aacctgggag acgcgggcgg
     2101 ccatcggcac ggccgagaca ccggccgaac cgatcagcgg gttgactttg ccctttgtcc
     2161 aggcgcacat gagcttaccg aacaggacgc cgctggcggt gccgcaggaa aacgcgaaca
     2221 ggccgagggc gatgatcatc agggtctgcg gctgcaggaa acgcgtggca ttcgccgtcg
     2281 ctcctaccga gacgccgagg aagatcgtca ggatattcat gagcgcgttc tgcgcggtat
     2341 ctgacagcct ggcggttact cccgactccc tgaacaggtt gccgagcata agggcgccga
     2401 ggagcggtac ggccgaaggc aggatgagcc cgatgaagat ggtcaccgca atcgggaaca
     2461 cgatcttctc ggtcttggtc acttcgcgca gctgctccat cttgatctgg cgctcgcg
//
LOCUS       DAOPHV010000403         2517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_58211_length_2517_cov_3.446791, whole genome shotgun sequence.
ACCESSION   DAOPHV010000403 DAOPHV010000000
VERSION     DAOPHV010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58211_length_2517_cov_3.446791"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLQ29_10015"
     CDS             complement(<1..250)
                     /locus_tag="PLQ29_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87022.1"
                     /translation="MDSSRMIDTARKATAALMSGRKPVFYASALIGLAAGAAALIMKA
                     LVHAVSAAGSGLVEAVGWPLLRYALPLVGIALTTVFTRY"
     gene            372..2045
                     /gene="ftcD"
                     /locus_tag="PLQ29_10020"
     CDS             372..2045
                     /gene="ftcD"
                     /locus_tag="PLQ29_10020"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPG87023.1"
                     /translation="MAERRIVECVPNFSEGRDKGVIDAIAKSIEGVQGVSLLDVDPGA
                     DTNRTVYTFVGESDAVLEAAVRSAATARSLIDMAKHSGAHPRMGALDVCPFVPVSGVT
                     MDECVALAKRAAERMAAELDVPVYLYEYAASRPERRSLADIRSGEYEALADKLARPEW
                     KPDYGPARFVPGWGATVVGARDFLIAYNVNLNTRDRKLANEVALNVREGGRAARDAAG
                     NIVKDGKGEKVMVPGRLKHVRAIGWYIDSYRRAQVSINLTNYKATPLHVVYETVKEEA
                     EKLGLSVCGSELVGLVPLEPLVEAGRYYLRRMGRSEGAPEPELVELAKTTLGLDSVAA
                     FDAERKIIDYAARPAAPLMAMTAAAFVDEVSSDSPAPGGGSVSALAGSLSAALSAMVA
                     NLTVGKKGYEPVWEPMSALAVRAQAVKQALTRAVDDDTSAFNAVMDAMKLPKGTAEEK
                     AAREAALQAGYKSAVEVPLATAEACVAALELAIEATAGNRNSSSDAGVAALLARAGAH
                     GAALNVLINLGSIADAAYVDGMKARVAALRTKADELCARAVAAVEATFA"
     gene            complement(2212..2502)
                     /locus_tag="PLQ29_10025"
     CDS             complement(2212..2502)
                     /locus_tag="PLQ29_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPG87024.1"
                     /translation="MADTILDDPIMRMIDGGAKVIDVRSPEEYDDEHYPNAINIPLNL
                     IQAKAAELGDKSAPLVLYCASGSRSAFAARMLSMAGFKNVVNAGGLYDMPGY"
BASE COUNT          434 a          833 c          850 g          400 t
ORIGIN      
        1 cgtagcgggt gaagaccgtc gtcagcgcta tcccgacgag gggcagggcg taacgtagga
       61 gcggccagcc gaccgcctcg acgagccccg agccggcggc cgagacggcg tgtacgagcg
      121 ccttcatgat cagggccgcg gcgccggcgg ccaggcctat gagcgcgctc gcgtagaaga
      181 ccggcttacg gcccgacatg agcgccgccg tcgccttcct cgcggtatcg atcatcctgg
      241 atgagtccat atcgatcagg atagcctcga tggacggcgg ggtaaaggcc tcgcgttctc
      301 acgaggcggc cgcccgtgct atactcggct tggcgcccct ccacggggcg gcgaaaaagg
      361 agatttctca gatggccgaa cgcaggatag tcgaatgcgt ccccaatttc agcgaggggc
      421 gcgataaggg cgtcatcgac gccatagcca agtcgatcga gggcgtccag ggcgtgtccc
      481 tcctcgacgt cgatcccggc gccgacacca atcgcaccgt gtacaccttt gtaggcgaga
      541 gcgacgccgt actcgaggcg gctgtccgtt ccgccgcgac ggcccgctcg cttatcgaca
      601 tggccaagca ctccggcgcc catccgcgca tgggcgccct cgacgtctgc cctttcgtgc
      661 cggtctccgg cgtgacgatg gacgagtgcg tcgccctggc caagcgggcg gccgagagga
      721 tggccgccga gctcgatgtg ccggtctacc tctacgagta cgccgccagc cgcccggagc
      781 ggcgtagctt ggccgacata cgctccgggg agtacgaggc cctggccgac aagctcgcga
      841 ggcccgagtg gaagccggac tacggcccgg cccgtttcgt acccggctgg ggcgccacgg
      901 tcgtaggcgc ccgcgacttc ctgatagcgt acaacgtcaa cctgaacacg cgcgatcgga
      961 agctagccaa cgaggtcgcg ctcaacgtgc gcgagggcgg cagggcggcc cgcgacgccg
     1021 cgggcaacat cgtcaaggac gggaagggcg agaaggtcat ggtacccggc cgcctcaagc
     1081 acgtgcgggc catcggctgg tacatcgact cgtaccgtag ggcccaggtg tcgatcaacc
     1141 tgaccaacta caaggccacg ccgctccacg tcgtctacga gacggtcaag gaggaggcgg
     1201 agaagctggg cctgtcggtc tgcggctccg agctcgtcgg cctcgtgccg ctcgagcccc
     1261 tcgtcgaggc gggccgctac tacctgcgcc gtatgggcag gagcgagggc gcgcccgagc
     1321 cggaactggt cgagctggcc aagacgacgc tcggcctcga ctcggtggcg gccttcgacg
     1381 ccgagcgcaa gatcatcgac tacgccgcga gacccgcggc cccgctcatg gcgatgacgg
     1441 ccgcggcctt cgtcgacgag gtgtcgagcg actcccccgc gcccggtggc ggctcggtct
     1501 cggcgctcgc cggctccctg tcggccgccc tgtcggctat ggtcgccaat ctaaccgtcg
     1561 gcaagaaggg ctacgagccg gtctgggagc cgatgtccgc cttggcggtc agggctcagg
     1621 ctgtcaaaca ggcgctcacc cgggccgtgg acgatgacac gagcgccttc aacgccgtga
     1681 tggacgcgat gaagctgccg aagggcacgg ccgaggagaa ggcagcgcga gaggcggccc
     1741 tgcaggccgg ctacaaatcg gcggtcgagg tgccgctggc caccgccgag gcctgtgtag
     1801 ccgcgctcga gctggctatc gaggctacgg ccggcaacag gaactcgtca tccgacgcgg
     1861 gcgtcgccgc gctcctcgcc agggccgggg cccacggcgc cgcgctcaac gtgctgatca
     1921 acctcggctc gatagccgat gcggcctatg tcgatggcat gaaggcccgc gtggccgcgc
     1981 tcaggaccaa ggccgacgag ctatgcgccc gggccgtagc cgccgtcgaa gccaccttcg
     2041 catagtgtgg atactctata aggcgatgaa gagaatacga ggatatgagg taggaaagag
     2101 aatctccgag taaaggtagc aatacgaaga ggcgaccagg ccttgggcct ggtcgcctct
     2161 tgctttacct ctggtgctcg tattcccttt gtgctgtatt ctacgtcgga ctcaatagcc
     2221 gggcatgtcg tagaggccgc cggcattgac cacgttcttg aagccggcca tcgacagcat
     2281 cctggcagcg aaggcgctcc tcgacccgga cgcgcagtac agcacgagcg gcgcggattt
     2341 gtcgcccagc tctgccgcct tggcctggat caggttgagc ggaatgttga tcgcgttcgg
     2401 gtagtgctcg tcgtcgtact cctcggggct ccgcacgtct atcaccttcg cgccgccgtc
     2461 gatcatcctc atgatgggat cgtctagtat cgtgtctgcc atggttcacc tccaaaa
//
LOCUS       DAOPHV010000404         2515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_58274_length_2515_cov_3.795122, whole genome shotgun sequence.
ACCESSION   DAOPHV010000404 DAOPHV010000000
VERSION     DAOPHV010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58274_length_2515_cov_3.795122"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PLQ29_10030"
     CDS             <1..272
                     /locus_tag="PLQ29_10030"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPG87025.1"
                     /translation="EPASAITAVRSGTVISAGPFRGFGLVAYVQAVDSKIYVYGGAAA
                     LSVKLGDPVRKGSLIGKVSSDGDASAYFFVFDGADTIDPASAPRD"
     gene            484..1500
                     /locus_tag="PLQ29_10035"
     CDS             484..1500
                     /locus_tag="PLQ29_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPG87026.1"
                     /translation="MNEMLLEKAAPAKRSKKTRSVLQQKKLKRPKTTKESDPLALYLK
                     QISRYPLLSAEEEVELGGAIQKTRNAVAALDSIADESDPDYGRNRAARAEKQAELVAY
                     KHRMISSNLRLVVSIAKNYQHRGLSLLDIIDEGNIGLIEAVERFDYTRGCRFSTYGTW
                     WIRQAIIKSLADKGRVIRIPIHMLNTIKKCYFVAKQLTQDLGRDPYAAELADKLGMDP
                     KKVKEIMKLSQETASLDTTVDEDAVTHLADLIKDENAVDPFEEVFCMTLQDTISDVLK
                     NLNQREITIITLRFGLNGEGPLTLEETGKLLGITRERVRQIQEKAVQKLKELRRLDEY
                     QEIC"
     gene            complement(1569..>2515)
                     /locus_tag="PLQ29_10040"
     CDS             complement(1569..>2515)
                     /locus_tag="PLQ29_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPG87027.1"
                     /translation="TALHLAVYWRATECLKLLARSASSLDPRDYTGKTPVRDAVDRSD
                     AQATAFLLERGADPLARDNSGETPLHAAARIADDRFTSALAAKAGRVDVRDDSGATPL
                     LEAVYAENARSARALASLGASIHSRDSAGESPLSYALKKGGAVLQALLDQKTARSSDA
                     DGRSVIRVILEAKPSIDFIELALAAGASVDDRDAFGRSPLHVAATNGYAEIAELLVAA
                     GADRFVRDSGGATPAVIALSRGDAMIAALFGASPNDADYLGETALHYAAAGGLDRAAQ
                     ALLALGADTARRNAAGETPADVAARRGHAALAKLLAGE"
BASE COUNT          495 a          768 c          797 g          455 t
ORIGIN      
        1 ccgaaccggc ctcggccatt accgcggtca ggtcgggtac ggtcatatcg gctggaccgt
       61 tccgtggctt cggtttggtc gcgtatgtac aggcggtgga tagcaagatt tatgtctacg
      121 gcggcgcggc ggcgctatcg gtcaagctcg gagacccggt gcgtaagggt tcgctgatcg
      181 gcaaggtctc atcggacggc gatgcgtcgg cctacttctt cgtcttcgat ggcgccgata
      241 cgatcgatcc ggcatccgca cctcgggact agcctatctg gtagacatcg agtctcgacg
      301 gtacaatcgg taaatattct tttacaatct aggctttgat atagtgaaat ttaatatttt
      361 tgataaaaaa tctcattttc ccttgattta ttcctccgga tgtataaaat tccacctgcg
      421 attcgatgcc tacgagggtt attcgccacg gcctaggctt agcggcctga ttcaggagac
      481 cgtatgaacg aaatgttgct tgagaaggca gcccccgcga agcgatctaa aaaaacgcga
      541 tcagtgctac agcaaaaaaa gctgaaacga ccgaaaacta ccaaggaaag cgatccgctc
      601 gccttgtacc tcaaacagat ttctaggtat ccgctgttaa gcgccgagga ggaggtggag
      661 ctcggcgggg ccatccagaa gacgaggaac gccgtggctg cgctcgactc gatagccgac
      721 gagtccgatc cggactacgg tcgcaaccga gccgcaaggg cggagaagca ggccgaactc
      781 gtcgcctaca agcaccgcat gatatcgagc aacctgcgcc tcgtcgtctc gatcgccaag
      841 aattaccagc accgaggcct gtccctgctc gatattatcg acgaaggcaa catcggcctg
      901 atcgaggccg tcgagcgctt cgactatacg cgcggctgca ggttttccac ctacggtacc
      961 tggtggattc gccaggccat catcaagagc ctggccgaca aggggcgcgt catacgcatc
     1021 ccgatccaca tgctcaacac catcaagaag tgctatttcg tcgcgaagca gcttacccag
     1081 gacctcgggc gcgatccgta cgccgccgag ctggctgaca agctcggcat ggatcccaag
     1141 aaggtcaagg agatcatgaa gctgtcccag gagacggctt cgctcgatac gacggtcgac
     1201 gaggatgcgg tcacccacct ggcggacctg atcaaggacg agaacgccgt cgatcccttc
     1261 gaggaggtct tctgcatgac gttgcaggat accatcagcg acgtgctcaa gaatctcaac
     1321 cagcgcgaga tcacgatcat cacattgcgt ttcggactga acggcgaggg gccgctgacg
     1381 ctcgaggaga ccggcaagct actcggcatc acgcgcgagc gcgtccgcca gatccaggag
     1441 aaggccgtcc agaagctcaa ggagctacgc cggctggatg aataccagga aatctgctga
     1501 taggcgacga tctcctataa aaaggggccg cccggatcga ttcctgacgg ccctgttttc
     1561 gtctcagctt actcgcccgc taggagcttc gccagagccg cgtgcccgcg gcgagccgcg
     1621 acgtcggccg gagtctctcc ggccgcgttg cgccgcgccg tatccgctcc gagggccaag
     1681 agggcctgcg ctgcccgatc gaggccgcca gcggccgcgt agtggagggc cgtctcgccg
     1741 aggtagtcgg cgtcgttagg cgaggcgccg aacagcgcgg ctatcatcgc gtcgcccctc
     1801 gacagcgcta tgacggccgg ggtggcgccg cctgagtccc gcacgaatcg gtcggcgccg
     1861 gccgcgacga gtagctcggc gatttcggcg tagccgttcg tcgccgcgac gtggagaggc
     1921 gaacgtccga aggcgtcgcg gtcgtcgacc gaggcgccgg ccgccagggc cagctcgatg
     1981 aaatcgatcg acggcttagc ctcgagtatc accctgatca ccgagcgtcc gtcggcgtcc
     2041 gagctcctgg ccgtcttctg gtcgagcagg gcttggagta ccgcgccgcc tttcttgagg
     2101 gcatagctga gggggctttc cccggccgag tccctcgagt ggatggacgc gccgagcgag
     2161 gcgagcgccc gagccgacct ggcgttctcg gcgtagacgg cctcgagcag gggggtagcg
     2221 cccgaatcgt ccctgacgtc gacacgcccg gccttggcgg ccagagcgct cgtgaagcga
     2281 tcgtcggcta tcctggccgc tgcatggagc ggcgtctcgc cggagttatc gcgcgccagc
     2341 gggtctgcgc cgcgctcgag caagaaggcc gtggcctggg cgtcggagcg gtcgacggcg
     2401 tcacgtaccg gcgtcttgcc ggtatagtcc ctcgggtcga ggctggaggc cgaacgggcc
     2461 agcagcttga ggcattcggt agcccgccaa tagaccgcca gatggagggc cgtcg
//
LOCUS       DAOPHV010000405         2510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_58429_length_2510_cov_4.657841, whole genome shotgun sequence.
ACCESSION   DAOPHV010000405 DAOPHV010000000
VERSION     DAOPHV010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58429_length_2510_cov_4.657841"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLQ29_10045"
     CDS             complement(<1..222)
                     /locus_tag="PLQ29_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87028.1"
                     /translation="MDPVSPALISAAILTLAAIVALGVSSGKHVKNASDFDTGGNRAG
                     PVMVAGTLVGTLVGGSATIGTAQLAFMYGL"
     gene            271..894
                     /locus_tag="PLQ29_10050"
     CDS             271..894
                     /locus_tag="PLQ29_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPG87029.1"
                     /translation="MTRKRARRSAMLAAACGLALVALLETAFGDREGSSADEAARRAT
                     ASADSELYTVPDRLRSLEALARRLGDMTSATVTRIVDGDTLVVELPGGSQEKVRLIGV
                     DTPESVDPRKPVERFSKEAAAYAERRLLGRDVRLAFGPEGRDYYGRLLAYVFLDDGTC
                     VNLAIVSDGYGFAYLKYPFPFQDEFSTAEREARRGGRGLWATSGETE"
     gene            complement(937..1269)
                     /locus_tag="PLQ29_10055"
     CDS             complement(937..1269)
                     /locus_tag="PLQ29_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPG87030.1"
                     /translation="MDQNETARELLALNMKRLRAAVGISQMVLAQRVGCSTTLIGNIE
                     IQKRFPSAENLDRIAGSFGVPVYELFIRDSPAIDRVASNFEVKESIERKVLEAIHGVL
                     DEKGFYYQ"
     gene            1715..>2510
                     /locus_tag="PLQ29_10060"
     CDS             1715..>2510
                     /locus_tag="PLQ29_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPG87031.1"
                     /translation="MSSQSRLTKVFCTALVLLVAFAGAVSAQGSLIRVGIVNLPPEES
                     GYRQANVEDMNAVFSRANGYDMKQTNTGDNSEQIAAAQGYIRDGVKYLLISAANASGW
                     DNVLKAAKTAGVTVFLFDRLIQTDASNYKAALVSDMAAEGTTAVNWLLSQKLPAYNVI
                     LIRGQMGSAAELGRSSALLKARDSGKVKIVADGTGGDSWSPEEARKVVEAAIAAGKDF
                     NVIYAQNDGMAQGAVQALDAAGISHGKNGKVKIIGFDFNRFALRNVQ"
BASE COUNT          516 a          782 c          781 g          431 t
ORIGIN      
        1 caggccgtac atgaacgcca gctgggcggt gcctatcgtc gcggacccgc cgaccagcgt
       61 accgaccagg gtgccggcga ccatgaccgg accggccctg ttcccgccgg tatcgaagtc
      121 ggaggcgttc ttcacgtgct tgccgctcga tacgcccaga gcgacgatgg ccgcgagcgt
      181 caggattgcg gcggagatca gcgccggcga tactgggtcc atgacgacga tgatatcgac
      241 gagcggggcg attggctata cgatatgcgg atgaccagga aacgagcgag gcgttcggcg
      301 atgctggcgg cggcctgcgg gctggccctc gtcgccctgc tggagacggc cttcggcgac
      361 cgcgaagggt cgtcagccga cgaggcggcg cgccgggcaa cggcgtccgc ggactccgag
      421 ctctacaccg tcccggacag gctccgttcg ctcgaggccc tggcgcgccg cctcggcgac
      481 atgacgagcg cgaccgttac caggatagtc gacggcgaca ccctcgtcgt cgagctgccg
      541 ggcggcagcc aggagaaagt caggctgatc ggcgtcgata ccccggagag cgtcgacccg
      601 cgcaagccgg tggaacgctt cagtaaggag gccgcggcct acgcggagcg gcgcctcctc
      661 gggcgggacg tccgcctggc cttcgggccg gaggggcgcg actactacgg gcgcctgctc
      721 gcctatgtat tcctggacga cggaacctgc gtcaacctgg cgatcgtatc ggacggctac
      781 ggcttcgcgt acctcaagta cccgttcccg ttccaggacg aattctccac cgcggagcga
      841 gaagccaggc gaggcggccg gggattatgg gcgacaagcg gcgagacgga gtaagctacc
      901 ggccgctacg acacttctat acggagcctt ccggcgttac tggtagtaga accctttctc
      961 gtcgagcacg ccgtggatgg cctcgagcac cttgcgctcg atgctttcct tgacctcgaa
     1021 gttcgaagcg accctatcga tagccgggga gtcccggatg aacagctcgt agaccggaac
     1081 gccgaaggag ccggctatac gatccaggtt ctcggccgag ggaaaccgct tctgtatctc
     1141 tatattgcct atgagcgtgg tggagcagcc gacccgctgc gccagaacca tctgcgatat
     1201 cccgacagcg gccctgagcc gcttcatgtt tagggcgagc aactctcgag cggtttcgtt
     1261 ctggtccaaa gcgttgagct cctcccgggt gctatagtgt caccccggaa aagccatgcc
     1321 tcaatcatcc cttggcggta ttagtttatt tctattaggc catcttcgta catcgaagca
     1381 ttcgccggaa gccaagatcg ataaggactg cgtgcgcggt accgccgaaa aacctatccg
     1441 aactaaaaaa agattgacag aaatacgaat gatagtacca tacgagtagc accgtgtacg
     1501 tacacgctac agagaacaga gtgtttcgta catacaccca atcccgatga gcggacagaa
     1561 tcgtccgact cggtatcgac gatggaagga ggagcgagcg gtctcaggat tccggtttca
     1621 gcgttgttac tacccgaggg cttcgaaagt caagcgaatg cgcggcgttg ccgccagaga
     1681 acgtgaagtt gttccgtagt atcggaggca tgaaatgtca tctcaatctc ggttaacgaa
     1741 agtattctgt acggccctag tactcctggt cgctttcgcc ggcgcggtca gcgcccaggg
     1801 ttccctgatc cgggtgggaa tcgtgaacct gccgcccgaa gaatccggct atcgccaggc
     1861 caacgtcgag gacatgaacg ccgtcttctc gagggccaac ggctatgaca tgaagcagac
     1921 caacaccggc gacaatagcg agcagatcgc ggcggcccag ggctatatcc gcgacggagt
     1981 caagtacctc cttatctcgg cggcgaacgc gtccggctgg gataacgtac tgaaggccgc
     2041 caagaccgcc ggcgtaaccg tattcctctt cgatcgcctc atccagaccg acgcctcgaa
     2101 ctataaggcc gccctcgtct ccgatatggc cgccgaggga accaccgccg tcaactggct
     2161 tctgagccag aagctacccg cctacaatgt gatcctcatc cgggggcaga tgggttcagc
     2221 ggccgagctt ggccgtagca gcgccctgct caaggcccgc gactcaggca aggtcaagat
     2281 cgtcgcggac gggaccggcg gcgacagctg gagccccgaa gaggcccgca aggtagtcga
     2341 ggccgcgata gccgccggca aggacttcaa cgtcatctac gcgcagaacg acggtatggc
     2401 ccagggcgcg gtccaggccc tcgacgccgc cggcatcagc cacggcaaga acggcaaggt
     2461 caagatcatc ggcttcgatt tcaaccgctt cgccctacgc aacgtacagg
//
LOCUS       DAOPHV010000406         2496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_58897_length_2496_cov_4.083982, whole genome shotgun sequence.
ACCESSION   DAOPHV010000406 DAOPHV010000000
VERSION     DAOPHV010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58897_length_2496_cov_4.083982"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PLQ29_10065"
     CDS             <1..486
                     /locus_tag="PLQ29_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPG87032.1"
                     /translation="KRGEFRSDLYYRLNVLPVYVPPLRQRKEDIPALAEFFLKKFSRE
                     TKKQFTGFTDQAIEHMLSYPWPGNVRELENAVERAVVIAKEKSIGARDLLIGDADTGR
                     DEYRGKSLKDALAIFKKHFIASALEEHGWNQTETSKVLDIQRTYLSRLIKEFAIANPK
                     E"
     gene            493..1212
                     /locus_tag="PLQ29_10070"
     CDS             493..1212
                     /locus_tag="PLQ29_10070"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG87033.1"
                     /translation="MSSKEIGATSATQAAPKDEVKFAERLGDFIRANRVGLLVVGVAI
                     LVAIVALGVYSIVSSDRLQKSTVALEALEAGFADWNALEGEARTAKGAELTAQADAVA
                     AKYGKLYAAGRASMIKAELLFAMSDLPGSEKAYLAAAAGAPKSHLAPVALANAASVAE
                     DRGDNEAALSYLQRAATEYPSAPAIGRVTLSIGRIYENMKRYGEAMEAYTRLIATGTE
                     SDWTKIAHARIILLKSRGLAD"
     gene            1319..>2496
                     /locus_tag="PLQ29_10075"
     CDS             1319..>2496
                     /locus_tag="PLQ29_10075"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HPG87034.1"
                     /translation="MPRKRSRILEAKRFSFVIAVLIFGVFSLLYFSTRIFQNLEGPIL
                     DSHFRLKTTKESKAIQEGAVLSHQNLQISDSILILGIDQKTLAEYGKWPFSRTRHADL
                     INAFSRIKAQENRESSLFVDVFFIEEDSDPTNDALLADAIGESGRVFLETVLSPYGSV
                     QEGDPMTVRQRALYDGSGSIKNVKGDWKKLVAFYGYEPPLEPFARAASGYGHANFMSD
                     SDQVYRRQPLVLRSSVLVETLRLDELRPGYAVDEASFERLSWQDDRGGYHDIDTPLTE
                     ATLAKLKDRMGDEAPLRIVDDDMDGTPDDSYYVVRRYRDTFVPAITLSLALNYFHRSL
                     VEVEVVIGSHILIPSPKVVDPDTGELVDYAIQTEPDQFDADGNLVAAGRRRTLPEIRI
                     P"
BASE COUNT          506 a          806 c          743 g          441 t
ORIGIN      
        1 aagcgcggcg agttccgtag cgacctgtac taccgcctga acgtcctgcc cgtctacgtg
       61 ccgcctctca ggcagcgcaa ggaggacata ccggccctgg ccgagttctt cctcaagaaa
      121 ttctcccgcg agaccaagaa gcagtttacc ggcttcaccg accaggctat cgagcacatg
      181 ctgtcgtacc cctggccggg aaacgtccgc gagctcgaga atgccgtaga gcgagccgtc
      241 gtcatcgcca aggagaaatc gataggcgcg agggatctgc tcataggcga cgccgatacc
      301 ggacgcgacg agtaccgcgg caagagtctc aaggacgcac tggccatctt caagaagcat
      361 ttcatagcga gcgcactcga ggagcacggc tggaaccaga ccgagacctc gaaggtactc
      421 gacattcaga ggacgtacct gtcccgcttg atcaaagaat tcgccatagc aaaccctaag
      481 gagtaagcat ctatgagctc taaggaaatc ggggccacgt ccgcgactca ggcggccccc
      541 aaggatgagg taaagttcgc cgagaggctc ggtgatttca tacgcgccaa tcgcgtcggg
      601 ctgttagtcg tcggcgttgc gatactggtc gccatcgtcg ccctcggggt ctactccatc
      661 gtctcgagcg acagactcca gaagtccacc gtcgccctcg aggccctcga ggccggcttc
      721 gccgactgga acgcgctgga gggcgaggcc cgtaccgcca agggtgcgga actgaccgcg
      781 caggccgacg ccgtcgccgc caagtacggc aagctgtacg ccgccggccg ggcctccatg
      841 attaaggccg agttactgtt cgccatgagc gacctgcccg ggtcggagaa agcctacctg
      901 gccgccgcgg ccggggctcc caagtcgcac ttggcgccag tcgcgctcgc caacgccgcc
      961 agcgtggccg aagatcgagg cgacaacgag gccgccctgt cgtacttgca gcgcgccgcg
     1021 acggagtatc cctcggcccc cgccatcggc cgcgtcacgc tatcgatcgg ccgcatctat
     1081 gagaatatga agcgctacgg agaggccatg gaagcctata cccgtctcat cgccaccggc
     1141 accgaaagcg attggacaaa gatcgctcat gcccgtataa ttctgctgaa gtctcgaggg
     1201 ctcgccgact aacgcgtccc cggcgaagtg ccgcggaagc ccggccggat ggccgggctt
     1261 ccgccgttta tggaccgttt ttcatatcag atccgaacac gttgctggag ggctcatcat
     1321 gccgaggaag cgctcacgaa tcctggaggc gaaacggttt tccttcgtca tagccgtgct
     1381 gattttcggc gtcttttcct tgctgtactt ctcgacccgt atcttccaga acctcgaagg
     1441 gcccatcctc gattcgcatt tcaggcttaa gacgaccaag gaatcaaagg ccatccagga
     1501 aggcgcggtg ctttcccacc agaacctcca aatctccgat agcatcctga tcctgggcat
     1561 cgatcagaag accctcgcgg aatacggtaa gtggccgttc tcgaggacac ggcatgcgga
     1621 cctgatcaac gcgttctcgc gcatcaaggc ccaggaaaat cgggagagtt ccctgttcgt
     1681 ggatgtgttc ttcatcgagg aggactctga tccgacgaac gatgccctgc tggccgacgc
     1741 catcggcgaa tcgggcaggg tcttcctcga gaccgtcctg tcgccgtacg gctccgtaca
     1801 ggagggcgac ccgatgaccg tcaggcagcg ggccctctac gacggctccg ggtccatcaa
     1861 gaatgttaag ggcgactgga agaagctcgt ggccttctat ggctacgagc cgccgctcga
     1921 gcccttcgcc agggcggctt caggctacgg ccacgccaac ttcatgtccg actccgacca
     1981 ggtctatcgt cgccagcccc tcgtgctgcg ctcgagcgtg ctggtggaga cgctccggct
     2041 cgacgagcta cggccgggct acgccgtcga cgaggcgtcg ttcgagcggc tgtcatggca
     2101 ggacgatagg ggaggctacc acgacatcga tacgcccctg accgaggcga cgctcgccaa
     2161 gctgaaggat cgcatgggcg acgaggcccc attacggata gtggacgacg acatggacgg
     2221 cactcccgac gatagctact acgtcgtcag gcggtatcgc gacactttcg tgccggctat
     2281 aaccctatcg ctggccctga actatttcca tcgctcgctc gtcgaggtcg aagtcgtcat
     2341 aggctcgcat atcctgatcc cttcgccaaa ggtagtcgac cccgataccg gcgagctggt
     2401 ggactacgcc atccagacgg agccagacca gttcgacgcc gacggaaacc tcgtggcggc
     2461 cgggcggcgt aggaccctgc cggagatacg catccc
//
LOCUS       DAOPHV010000407         2494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_58964_length_2494_cov_4.298893, whole genome shotgun sequence.
ACCESSION   DAOPHV010000407 DAOPHV010000000
VERSION     DAOPHV010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58964_length_2494_cov_4.298893"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PLQ29_10080"
     CDS             complement(<1..456)
                     /locus_tag="PLQ29_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA (guanine-N2)-methyltransferase"
                     /protein_id="HPG87035.1"
                     /translation="MPNEKDLEQAGFFANRLLKRDRHLRKWARRTDTNCYRVYDRDIP
                     ELPLSLDRYGEAAVLYLYERPYEKPDDEERAWLELMAEAASAALGIEPGSVAIKTRRR
                     LGVDKQYGKAEDDVGAYVRPGSSSGPGELTVKENGLSFIVNTRDYIDTGL"
     gene            complement(446..1102)
                     /locus_tag="PLQ29_10085"
     CDS             complement(446..1102)
                     /locus_tag="PLQ29_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPG87036.1"
                     /translation="MARFKVSYAPSVRRLPSYLLLAKQAQDHGDEYISGTMIAIELNL
                     EPIQVRKDLSITGIVGKPKRGYPVGPLIAAIESYLNWDKRVDAVVVGAGNLGSALTGF
                     PGFKQHGLHIVAGFDADPAKVGATIYDTPVYAMEQLDERIKSLGVTVAVLTVPSIYAQ
                     DIAARLVAAGITAIWNFTNVKLKIPDEVIVQNEDLSSGYAMLSIKRHLRGLMKTADAE
                     "
     gene            1467..1892
                     /locus_tag="PLQ29_10090"
     CDS             1467..1892
                     /locus_tag="PLQ29_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPG87037.1"
                     /translation="MRITTKGRYALRAVVALARLSDGSSPVSIKTIGGEEGLSAEFLE
                     QIFFRLKKAGIIRSIRGPGGGFVFAKPLSAILLKDIVMASGEGVDLATCSGDGDEERC
                     DRVDRCAAGRVWRELSDYIDTYLTSVSLEQILAQDGRKA"
     gene            complement(1909..>2494)
                     /gene="pyrE"
                     /locus_tag="PLQ29_10095"
     CDS             complement(1909..>2494)
                     /gene="pyrE"
                     /locus_tag="PLQ29_10095"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPG87038.1"
                     /translation="RSIMRALVETGCFRTGSFTLKSGKLSPFYIDLRRVISDPALLDL
                     VASAYAMTAEGVAYDRIAGIPAAALPLATAASLKLRAPMVWPRMPAKDHGTGVRVEGA
                     FKPGERVLLLDDLITTGASKLEAIQILRGEGLVVEELAVLIERGTQGRADMESCGVRL
                     RAFMHVRELIALCRDMALIDDAERERLEAFIDGE"
BASE COUNT          492 a          748 c          781 g          473 t
ORIGIN      
        1 gagcccggta tcgatatagt ccctggtatt gacgatgaag gaaaggccgt tttctttgac
       61 cgtcaattcg ccgggtcccg aagacgagcc ggggcggaca tacgctccga cgtcgtcttc
      121 ggccttgccg tactgcttgt cgacgccgag ccttctgcgc gtcttgatgg cgaccgagcc
      181 gggctctata ccgagcgcgg ccgaggcggc ctcggccatc agctcgagcc aggcgcgctc
      241 ctcgtcatcg ggcttctcgt aggggcgctc gtacaggtat aggaccgccg cctcgccata
      301 gcggtcgagg gatagcggca actcgggtat atcgcggtca tagacgcggt agcagttcgt
      361 atcggtcctg cgggcccatt tccgtaggtg ccgatcgcgt ttcaggagcc tgttggcgaa
      421 gaaaccggct tgctccagat ctttttcatt cggcatcggc cgtcttcatg aggccgcgca
      481 gatgacgctt gatcgacagc atagcgtaac cggatgacag atcctcgttc tgcacgatca
      541 cctcgtcggg gatcttgagc ttgacgttcg tgaagttcca gatggcagta atgcccgcgg
      601 cgacaagcct ggcggcgata tcctgagcgt agatcgaagg gaccgtcagg acggctaccg
      661 ttaccccgag cgacttgatg cgttcgtcga gctgttccat ggcgtacact ggcgtgtcgt
      721 agatggtagc acccactttc gcgggatcgg cgtcgaagcc ggccacgata tgcagcccgt
      781 gctgcttgaa gccagggaat ccggtcaggg cgctaccgag attgcccgct ccgaccacga
      841 ccgcgtcgac gcgcttgtcc cagttcaggt acgattcgat cgccgcgatc aggggcccca
      901 cgggatagcc gcgtttcggc ttgccgacga tgccggtaat cgagaggtcc ttgcgaacct
      961 ggatcggctc gagattcagc tcgatcgcta tcatcgtgcc ggaaatatac tcgtcaccgt
     1021 gatcctgagc ctgcttcgcg agcaagagat acgagggtag ccttcgcacg gacggagcgt
     1081 acgaaacctt aaatcgcgcc atggaacacc tctcgtgcga aaagtataga taccagcact
     1141 acattggaat gaagaatagt cagaagcgtc tagtaaagtc aagaatataa ggttttttat
     1201 atagcgtttc gctatcgaca cgaccggcgt caaggcatag gaaggccccg tggaaaaagg
     1261 aggtaaacca cggggccggt tgtagcgccg gagcaactac agaataaata tacaagctat
     1321 gctatgatta tgcaagcggt atcggtggac gagcccatcg agtcgcccgc cgcgaagcta
     1381 tgtcttctct atcgagcata ttcatagtag gatcacaggc ccggacgcat cccgagcgta
     1441 aacgcgccga ttcgaggaga gaacgcatga ggattacgac gaagggccgc tacgcgctca
     1501 gggcggtcgt ggccctcgcc aggctgagcg acggatcctc ccccgtctcg atcaagacca
     1561 tcggcggcga agagggttta tcggccgagt tcctcgagca gatcttcttc aggctcaaga
     1621 aggcgggcat catccggtcg atccggggtc ccggcggagg ctttgtcttc gcgaagccgt
     1681 tatcggctat actcctcaag gacatcgtca tggcttccgg cgagggggtc gacctcgcca
     1741 cctgctcggg cgacggcgac gaggagcgtt gcgaccgggt cgatcgctgc gcggccggca
     1801 gggtctggcg ggaactgtca gactatatcg acacgtacct cacgagcgtc agcctggagc
     1861 agatcctggc acaggacggc cggaaagcct aggccgtccc cggatagcct actcgccgtc
     1921 gatgaacgcc tcgagccgct cccgctccgc gtcgtctatc aaggccatat cgcggcagag
     1981 cgctatgagt tccctgacgt gcatgaacgc gcgtaacctg acgccgcacg actccatgtc
     2041 cgctcgcccc tgcgtcccgc gctctatcag caccgcgagc tcttcgacga cgaggccctc
     2101 gccccgtagg atttgtatcg cttcgagctt cgacgcgccg gtcgtgatca ggtcgtcgag
     2161 cagcaggacc cgctctccgg gcttgaacgc gccctcgacg cggacgccgg taccgtggtc
     2221 cttggccggc atgcgtggcc ataccatggg cgcgcgtagc ttgagcgagg cggcagtggc
     2281 caaaggcagg gcggccgcgg gaatgccggc gatacggtca taagcgacgc cctcggccgt
     2341 catcgcgtag gccgaagcca ccaggtcgag gagcgcgggg tcggagatca cgcgccgcag
     2401 gtcgatatag aacggcgaga gtttaccgct cttgagcgtg aacgagccgg tcctgaagca
     2461 accggtctcg acgagagcgc gcatgatcga tcgc
//
LOCUS       DAOPHV010000408         2494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_58970_length_2494_cov_3.896269, whole genome shotgun sequence.
ACCESSION   DAOPHV010000408 DAOPHV010000000
VERSION     DAOPHV010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58970_length_2494_cov_3.896269"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLQ29_10100"
     CDS             complement(<1..376)
                     /locus_tag="PLQ29_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87039.1"
                     /translation="MRVRVDAARYREAQELEGRGVTALAVTPGFLRSEWMLDYFGVTE
                     ANWRDATKNVREFIASETPLFVGRCVAALAADPDVARKNGRVFASWDLGEEYAIDDAD
                     GSRPHFVRWIREHMPEVAAGWKK"
     gene            377..505
                     /locus_tag="PLQ29_10105"
     CDS             377..505
                     /locus_tag="PLQ29_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87040.1"
                     /translation="MDYVAAALANVKERADGIRRGYRVAWEAPIMRHFTVELTPAK"
     gene            825..2048
                     /locus_tag="PLQ29_10110"
     CDS             825..2048
                     /locus_tag="PLQ29_10110"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HPG87041.1"
                     /translation="MSRYPDAFIREIPKTDLHLHLDGSLRLSTLIEMARKGGVELPSY
                     AEDGMRELVFKDRYADLGEYLAGFSYTCAVLRDPENLERAAYELAVDCIDEGVRYIEP
                     RFAPQLLMDNRDLTMDRILAAVDKGLARAKAEFNARPEVADEGLPPFDYGIIVCAMRA
                     FGERGFSPYYDAFYGIHRYADRAEVIRMAAVEAARGAVRIRDELGIPIVGFDIAGQEA
                     GHPAGQFREAFDFVHQNFMHKTVHAGEAYGAESIFQAITDLHADRLGHGYYLFDPDKV
                     AATSVRDKRRYIERLAGFVADQRVTIEVCLTSNLQTNPSLERVADHSFGEMLERRLSA
                     TLCTDNRLVSNTTVSKEIRLAVDNFDIGPSALKNLIVYGFKRSFYPGPYAAKREYVRR
                     AITRYEAVARKYGVE"
     gene            2132..>2494
                     /locus_tag="PLQ29_10115"
     CDS             2132..>2494
                     /locus_tag="PLQ29_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87042.1"
                     /translation="MERTSARSPQAPALLLISGLGHGLALAYASLMPRAAEPHAGPAY
                     FIILIAASAVLVVPLARWAGDPAAAALALAAKLGLAILALIPIVAPSPLKALILSVLV
                     AEAVLVLPRPWDLAGAAIA"
BASE COUNT          421 a          910 c          805 g          358 t
ORIGIN      
        1 ccttcttcca cccggcggcg acctcgggca tgtgctccct gatccagcgc acgaagtggg
       61 gtcgtgagcc gtcggcgtcg tcgatggcgt actcctcccc gaggtcccac gacgcgaaga
      121 cccggccgtt cttcctggcg acgtccggat cggccgcgag cgcggcgaca cagcggccga
      181 cgaagagcgg agtctccgag gcgatgaact ctctcacgtt cttcgtggcg tcgcgccagt
      241 tcgcttcggt cacgccgaaa tagtcgagca tccactccga gcggagaaaa cccggtgtga
      301 cggcgagcgc ggtgacgccc cggccctcga gctcctgcgc ctcgcgatac cgcgccgcgt
      361 ctacacgaac gcgcacatgg actacgtggc ggccgccctc gccaacgtga aggaacgcgc
      421 cgacggcatc aggcgcgggt accgcgtcgc ctgggaagcc cccatcatgc gccacttcac
      481 cgtagagcta acgccagcaa aatagcctat ccggccgccg ccacgcaccg aacgtgaagt
      541 ggcggcgcgc cagggatggc gcgggccgct ttcagcggcc acgaccatca tttcctatcg
      601 gaatccgggc atgcaccgca tgcccggatg gcgcgcccaa gggcggcccg tccgcaggac
      661 gggaacgtca cggatgacga cgcgccaggg ttggcgcgcc caagggcggc ccgtccgcag
      721 gacgggaacg tcatggatga cgatacgtca tggatgacga cgctcctcga ctcgcgaggc
      781 cgagacggct acactaggag gtagcgccaa ccgaggagac accagtgagc cgataccccg
      841 acgccttcat ccgcgagatc cccaagaccg acctgcacct ccacctcgac gggtcgctgc
      901 gcctatccac cctgatagag atggcccgta aaggcggcgt cgagctgccc tcgtacgccg
      961 aggacggcat gcgcgagctc gtcttcaagg accgctacgc cgacctgggc gagtacctgg
     1021 ccggtttctc gtatacctgc gcggtgctgc gcgacccgga gaacctcgag agggcggcct
     1081 acgagctcgc cgtcgactgc atcgacgagg gcgtgcgcta catcgagccg cgcttcgctc
     1141 cgcagctgct gatggacaac cgcgacctga cgatggaccg catactcgcg gccgtcgaca
     1201 agggactggc ccgcgccaag gccgagttca acgcccggcc cgaggtggcc gacgaagggc
     1261 tgccgccgtt cgactacggc atcatcgtct gcgcgatgcg ggcgttcggc gagcgcggct
     1321 tctccccgta ctacgacgcg ttctacggga tccaccgcta cgccgaccgg gccgaggtga
     1381 tacggatggc cgcggtcgag gcggcccgcg gcgcggtcag gatacgcgac gagctcggga
     1441 tccccatcgt cggcttcgac atcgccgggc aggaagccgg ccacccggcc ggccagttcc
     1501 gcgaggcctt cgacttcgtc caccagaact tcatgcacaa gaccgtgcac gccggcgagg
     1561 cctacggcgc ggagagcatc ttccaggcca tcaccgacct gcacgccgac aggctcgggc
     1621 acggctacta tctcttcgac ccggacaagg tcgccgcgac gagcgtccgg gacaagcgcc
     1681 gttacatcga gaggctggcc ggcttcgtcg ccgatcagcg cgtgaccatc gaggtctgcc
     1741 tgacgagcaa cctgcagacg aacccctcgc tcgagcgcgt ggccgatcac tccttcggcg
     1801 agatgctcga gcgccggctg tcggccaccc tgtgcaccga caaccggctg gtatccaaca
     1861 cgaccgtctc caaggagatc cgactggcgg tcgacaactt cgacatcggc ccgtcggccc
     1921 tcaagaacct gatcgtctac ggcttcaagc gcagcttcta ccccggcccc tacgcggcca
     1981 agcgcgagta cgtacggcgc gcgatcaccc gctacgaagc ggtcgccagg aaatacggcg
     2041 tcgagtgagc gtggacggcc cgaggcgggc gacaccggcc cgctccgagc gttgacagaa
     2101 ccgcgctacg gctacagact atcgtccacc gatggagcgc acgtcagcac gatcgccgca
     2161 ggcccccgcc ctgctcctca tctcgggcct cggacacggc ttggccctcg cctacgcgag
     2221 cctgatgccg agggcggcgg agccgcacgc cggaccggcg tatttcataa tcttaatcgc
     2281 cgcgagcgcg gtcctggtgg tgccgctcgc ccgctgggcg ggcgatcccg cggccgcggc
     2341 cctggcgctc gccgccaagc tcggcctggc gatactcgcg ctgatcccga tcgtcgcccc
     2401 gagcccgctc aaggcgctca tcctgtcggt cctggtcgcg gaggcggtcc tcgtcctgcc
     2461 gcggccctgg gacctggccg gcgcggccat cgcc
//
LOCUS       DAOPHV010000409         2493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_59004_length_2493_cov_4.318704, whole genome shotgun sequence.
ACCESSION   DAOPHV010000409 DAOPHV010000000
VERSION     DAOPHV010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59004_length_2493_cov_4.318704"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2493)
                     /locus_tag="PLQ29_10120"
     CDS             complement(<1..>2493)
                     /locus_tag="PLQ29_10120"
                     /inference="COORDINATES: protein motif:HMM:NF037694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HPG87043.1"
                     /translation="LDGSLRSDDAPASADVERLVSATLGGRRLDLSWSHEGDGLHRFT
                     VKRIPRSVRESTLTLSWDGQIIGASAKGSERYRIPAEGSFELLSVTGPEPGESACLRL
                     AFSEPVDKAQDFRGLIRTSSSGTSSPGSLRYESEGGLVRVYSSLGWSEEVGVTVEKGL
                     RGLSGGAIAVPVQATVRFDWEKPEVRFQAGGVIVPSTQGTRVVLETRNLSKVVVEALR
                     VYGDNMLQFLQVNDLDGSKELKRVGEVVWREEIDLGWTDDKKNQWTPYALDLSPLLAK
                     HPDGLFQLRVAFGRDHIRYVSPNDHASLGKWTFPPVSIRDSDDDSSYWDYYETWFDWE
                     QYYRYRDDPTHPAFYVQRYGRDRTARRNVLVSDVALSAKLDVDGAWHLVAGDLRGAKP
                     LSGARVSLYSYAMRELASATSDASGLAVLKPKAGESAQPYFAVAEAAGAKTGRERGYL
                     KLTPSSALAVSHFDVGGETAESGVKGFIYGERGVWRPGDDMHLCFVLYDRLKTLPADH
                     PVRFELESPLGQVTRQATYTESVGGFYYIKTGTDPSAPTGTWTARVTVGGKSFTKAVK
                     VETVMPNRLKLELDYGASPYVGADLREMGLKAAWLHGAPAPGLKADVSMTLSASSKAP
                     GDYPGFSFQDPLRTAPSARELLFEGYLDGSGSARFRVDLAPEAQAPGPLTASFLSRAF
                     ERSGLFSSEQFSVDFHPYRRYVGVKVPEGDASRGMLLTDRDHPVEILLVDRDGKPSGD
                     GQVEVSLYKLEWRWWWEKGSESLAEQASDIYSRRISKELVNVKNGRASWKLRINYPEW
                     GRYMLRVADLSGSSAATGAAATGAAATGGNHA"
BASE COUNT          398 a          831 c          861 g          403 t
ORIGIN      
        1 cggcatggtt gccgcctgtg gcggcagcgc ctgtggcggc agcgcctgtg gcggcgctcg
       61 agccggacag gtcggcgacg cgcagcatgt agcgccccca ctcggggtag ttaatcctga
      121 gcttccagga ggcgcggccg ttcttgacgt tgacgagctc cttgctgatg cgcctgctgt
      181 agatatcgga agcctgctcg gcgaggctct cgctaccctt ctcccaccac cagcgccact
      241 cgagcttata gagcgacacc tcgacctggc cgtcgccgga aggcttgcca tcgcggtcga
      301 cgagcaggat ctccacggga tggtcgcggt cggtgagcag catacccctg gacgcgtctc
      361 cctcgggcac cttgacgcca acgtaacgcc ggtacggatg gaagtcgacc gagaactgct
      421 cgctcgagaa cagacccgac cgctcgaaag ccctcgacag gaagctggcc gtaagcgggc
      481 caggggcctg agcctcgggc gccaggtcga ctctgaagcg ggccgagccc gaaccgtcca
      541 ggtaaccttc gaagagcagc tcgcgcgcgc tgggggccgt gcgtagcgga tcctggaacg
      601 agaagcccgg gtagtcgccg ggcgccttgg acgaggccga cagcgtcatc gacacgtccg
      661 ccttgagtcc gggcgccggc gcgccatgga gccaggcggc cttgagcccc atctcgcgca
      721 ggtcggctcc gacgtagggt gaagcgccgt agtccagctc cagcttgagc cggttcggca
      781 tgacggtctc gaccttgacg gccttggtga agctcttgcc gccgaccgtg acgcgggccg
      841 tccaggtacc ggtcggtgcg ctcgggtcgg tacccgtctt gatgtaatag aagccgccga
      901 cagattcggt ataggtggcc tgcctggtga cctggccgag cgggctctcg agctcgaagc
      961 ggaccgggtg gtcagccggc agggtcttca ggcgatcgta cagcacgaag cacaagtgca
     1021 tgtcgtcgcc cgggcgccac acgccgcgct cgccgtagat gaaacccttg acccccgatt
     1081 cggcggtctc gccgccgacg tcgaaatggc tgaccgcgag ggcgctcgac ggggtcagct
     1141 tgaggtaacc gcgctcgcgg ccggtcttgg cgccggccgc ctcagccacc gcgaagtacg
     1201 gctgggccga ctccccggcc ttgggcttca gcaccgcgag gccggaggcg tcggacgtgg
     1261 ccgaggccag ctcgcgcatc gcgtagctat agagtgagac gcgcgccccg gacaggggct
     1321 tggcgccgcg caggtcgccg gcgacgaggt gccaggctcc atcgacgtcg agcttggccg
     1381 acagggccac gtccgacacg agcacgttgc gcctggcggt gcggtcgcgg ccgtagcgct
     1441 gcacgtagaa ggccggatga gtcgggtcgt cgcggtaacg gtagtactgc tcccagtcga
     1501 accacgtctc gtagtaatcc cagtacgacg agtcgtcgtc cgagtcgcgt atgctcaccg
     1561 gcgggaaggt ccacttgccg aggctggcat ggtcgttcgg gctgacgtag cgtatatggt
     1621 cgcgtccgaa ggcgacgcgc agctggaaca ggccgtccgg atgcttggcc aggagcggcg
     1681 acagatcgag cgcgtagggc gtccactggt tcttcttgtc gtccgtccag cctaggtcta
     1741 tttcctcccg ccagacgacc tcgcctaccc gcttgagctc cttggacccg tccaggtcgt
     1801 tgacctgcag aaactggagc atattgtcgc cgtagacccg tagggcttcg acgaccacct
     1861 tggagaggtt cctggtctcg aggacgacgc gggtgccctg ggtcgacggc acgatgacgc
     1921 cgccggcctg gaagcggacc tccggcttct cccagtcgaa gcggaccgtc gcctggaccg
     1981 gtacggcgat ggcgccgcct gataggcctc gcaacccctt ctccacggtc actccgacct
     2041 cctcgctcca gccgagcgac gagtaaacgc gcacgaggcc tccctcgctc tcgtagcgca
     2101 gcgagccagg cgacgatgta ccggaagacg aggtccgaat gagtccgcgg aagtcctggg
     2161 ccttgtcgac cggctcgctg aaggccagcc tgagacaggc ggactcgcct ggttcggggc
     2221 cggtcaccga taggagctcg aagctacctt cggccggtat cctatagcgc tccgagccct
     2281 tggccgacgc gcctatgatc tggccgtccc agctgagcgt cagggtggac tcgcgcaccg
     2341 agcgcggtat gcgcttgacc gtgaagcggt ggaggccgtc gccctcgtgg ctccaggaca
     2401 ggtcgagccg gcggccgccc agggtcgccg agacgagccg ctccacatcg gccgaggccg
     2461 gcgcgtcgtc ggatcggagc gagccgtcga gcg
//
LOCUS       DAOPHV010000410         2493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_59011_length_2493_cov_3.657096, whole genome shotgun sequence.
ACCESSION   DAOPHV010000410 DAOPHV010000000
VERSION     DAOPHV010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59011_length_2493_cov_3.657096"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PLQ29_10125"
     CDS             complement(<1..791)
                     /locus_tag="PLQ29_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014653446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPG87044.1"
                     /translation="MKGIIVAAGYGTRFLPVTKTVPKEMLPLINKPSIAFVVDEFVAS
                     GIEDIVIITSRRKKSLDDYFDHEAELEAVFAREGRDDKAALIAPPAARISFVRQTEMR
                     GTGHALLQAAPLLGGEPCVVAYPDDLHFGAPPLARQLMDVHERTGGCVLATVHDPGDV
                     SRYGVVDPAADGEGVRGFVEKPTKGSEPSHEISIGRYLYTPEFFDLLAEGWKRHEDGE
                     YYHIYALDRMIAAGKVAYKSVEGQRLDTGEPAGYLEAVLEYAWREP"
     gene            947..1612
                     /locus_tag="PLQ29_10130"
     CDS             947..1612
                     /locus_tag="PLQ29_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPG87045.1"
                     /translation="MKLLCIADTVDPLVDSPAVRERFGDVDLTLGAGDLPMEYLSYVV
                     SALNRPLLFVFGNHNLGDLPHYRPAPGSPPPSPHDLSRDVGATYVGFKLLREADLIVM
                     GLGGSLRYNDGQNQFTQAEMWARVLAKIPALLWNRLVRGRAVDVVLTHAPPRGIHDRP
                     DRCHRGFDAFLWLMRAFRPRFLVHGHVHLYDAKERRVTRYRDTVVVNAYGHAVLDTEA
                     SYD"
     gene            1605..>2493
                     /locus_tag="PLQ29_10135"
     CDS             1605..>2493
                     /locus_tag="PLQ29_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPG87046.1"
                     /translation="MTDPLDELAASDFSRARLREALTRVGGFLRPEHQRLLSLDEVRS
                     ILKIRGEEYRGVMPVPLELIIGSEGRYRDFARGFLPRHDYLRDRWIRVDSAHYHDVIL
                     PPVRLYELGGAYFVRDGNHRVSVAKLQGALEIDAEVTSLGSAVRIDKGMDMDALRRAI
                     VGYEKREFYAQTLFGQVTDDPALDFTAPGAYDQIVEHVLVHKYYLNERSPEEISFVDA
                     LDSWYRAVYRPIIEAIEAEGLLSGFRGRTASDLYLYLVKHWDELKRRYGLHYPVSAAA
                     RDFRDRYGRGFLKDAKAFLG"
BASE COUNT          390 a          882 c          825 g          396 t
ORIGIN      
        1 ggctcgcgcc aggcgtactc gagcacggcc tccaggtagc cggccggctc gccggtatcc
       61 aggcgctggc cctcgacgct cttgtaggcc accttgccgg ccgcgatcat ccgatccagg
      121 gcgtagatat ggtagtactc gccgtcctcg tgccgcttcc agccctcggc gagcaggtcg
      181 aagaactccg gcgtgtagag gtagcggcct atcgatatct catggctcgg ctcggaaccc
      241 ttcgtcggct tctcgacgaa accgcgcacg ccctcgccgt cggcggccgg gtcgacgacg
      301 ccataacgcg acacgtcgcc cggatcgtgg acggtcgcca gcacgcagcc gccggtacgt
      361 tcgtgcacgt ccatcagctg gcgcgcgagc ggcggcgcgc cgaagtgcag gtcgtccggg
      421 taggccacga cgcagggctc gccgccgagg agcggcgccg cctgcagcag ggcgtggccg
      481 gtgccgcgca tctcggtctg gcgcacgaac gagatacggg ccgcgggcgg cgctatcagc
      541 gcggccttgt cgtcgcgccc ctcgcgggcg aagacggcct cgagctcggc ctcgtggtcg
      601 aagtagtcgt cgagcgactt cttgcggcga ctcgtgatga tgacgatgtc ctcgatgccc
      661 gacgcgacga actcgtcgac gacgaacgct atcgagggct tgttgatcag ggggagcatt
      721 tccttgggca cggtcttggt gaccgggagg aaacgggtgc cgtaaccggc ggcgacgatg
      781 atgcctttca tgcctccaat gatacccgag cctggccggc tcgccaaggg cgacccgccg
      841 gaggcgcccc gccggaggcg ccccgccgga ggcgccccgc cagaggcgcc ccttgacccc
      901 gttcggccgc ggagccgata ctgaccgcca ggaggctccg gaccggatga agctactctg
      961 catagctgat accgtcgacc cgctcgtcga ctcgccggcc gtacgagagc gcttcggcga
     1021 cgtcgacctg acgctcggag ccggcgacct gccgatggaa tacctatcgt acgtcgtctc
     1081 ggcgctcaac cggccgctcc tgttcgtctt cggcaaccac aacctcgggg atctgcctca
     1141 ttaccggccc gctcccggct cgccgccgcc gtcgccccac gacctgtcca gggacgtcgg
     1201 cgccacctac gtcggcttca agctgctgcg cgaagctgac ctgatcgtca tgggcctcgg
     1261 aggctcgcta cgctacaacg acggccagaa ccagttcacc caggccgaga tgtgggcccg
     1321 cgtgctggcc aagatcccgg cgttgctgtg gaaccgcctg gtccgcggcc gggccgtgga
     1381 tgtggtgctg acgcacgcgc cgccgcgcgg catccatgac aggccagacc gctgccaccg
     1441 cggcttcgac gcgttcctct ggctgatgcg ggccttcagg ccgcgcttcc tcgtgcacgg
     1501 ccacgtgcat ctctacgacg ccaaggagcg gagggtaacg cgctaccgcg acacggtcgt
     1561 ggtcaacgcg tacggccacg cggtcctgga cacggaggcg agctatgact gacccgctcg
     1621 acgagctggc ggccagcgac ttctccaggg cccggctacg cgaggcgctg acgcgcgtcg
     1681 gcggcttcct ccggccggaa caccagcgcc tgctgtcgct cgacgaggtg cgttcgatac
     1741 tcaagatacg cggcgaggag taccgcggtg tgatgccggt gccgctcgag ctgattatag
     1801 gctcggaggg ccgctaccgc gacttcgcgc gcggcttcct gccccggcac gactacctgc
     1861 gcgaccgctg gatacgcgtc gactcggccc actaccacga cgtgatcctg cctccggtca
     1921 ggctctacga gctcggcggg gcgtacttcg tgcgcgacgg caaccaccgc gtatcggtgg
     1981 ccaagctgca gggcgcgctt gagatagacg ccgaggtcac ctcgctcggc tcggccgtgc
     2041 gcatcgacaa gggcatggac atggacgccc tgaggcgggc catcgtcggc tacgagaagc
     2101 gggagttcta cgcgcagacc ctgttcggcc aggtgacgga tgacccagcc ctcgacttca
     2161 ccgcgccggg cgcctacgac cagatcgtcg agcacgtgct ggtccataag tactacctga
     2221 acgagcgatc gcccgaggag atcagcttcg tcgacgcgct cgactcctgg taccgagcgg
     2281 tctacaggcc catcatcgaa gccatcgagg cggaagggct gctgtcgggc ttccgcggac
     2341 gcaccgccag cgacctctac ctgtacctcg tcaagcattg ggacgagctc aaacggcgct
     2401 acggcctgca ctaccccgtc tccgcggccg cccgcgattt cagggaccgc tacggccgcg
     2461 gattcctgaa ggacgccaag gccttccttg gct
//
LOCUS       DAOPHV010000411         2487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_59202_length_2487_cov_4.500000, whole genome shotgun sequence.
ACCESSION   DAOPHV010000411 DAOPHV010000000
VERSION     DAOPHV010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59202_length_2487_cov_4.500000"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLQ29_10140"
     CDS             <1..305
                     /locus_tag="PLQ29_10140"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HPG87047.1"
                     /translation="WGAAALGIALKPFLTGKMKFVSTVAYVLMGWIIVFAWKPLAAAV
                     DVAALRYLVAGGLAYTIGAAFYLLKRRCWTHPVWHLFVGAGATLHFFAALALLPQG"
     gene            complement(296..763)
                     /locus_tag="PLQ29_10145"
     CDS             complement(296..763)
                     /locus_tag="PLQ29_10145"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HPG87048.1"
                     /translation="MSAQAVQLAWAFLATLGFGVLFRAPLRDLPFTALGGAVSWGAYL
                     AFKAATGSDTLSYFAASVAVGLYAEAMASCLKRPATLFILSAIIPLVPGAGMYRTMLE
                     AVNGRAAASASVGFETLTLAGAIAGGLAVASAASRILWLKKPRLPRRPGSRQP"
     gene            complement(760..1548)
                     /locus_tag="PLQ29_10150"
     CDS             complement(760..1548)
                     /locus_tag="PLQ29_10150"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HPG87049.1"
                     /translation="MTTDDEDNTDDALRLSAEAARIVLESGGETYRAEEVMSAVASAL
                     GALEPEAFATPTGFMASCDDRYGRGRAVVRRIRKHQLNLDKITRVNALARDAVSGRLD
                     RDGIERELCAIDALGAYPRPIPAVGAAFVTGFFCLLFGGAWNDALVSAGIGLVIGRIT
                     SALGGMKLSVFFSNIVGGAFAAAVAIGAEALRVAGNTDAMIIGSIMLLVPGVLIVNAI
                     RDLIAGDLVAGIARAADAFISAAGISIGVGLALQLAMLISGPTP"
     gene            complement(1545..2207)
                     /locus_tag="PLQ29_10155"
     CDS             complement(1545..2207)
                     /locus_tag="PLQ29_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87050.1"
                     /translation="MKRIVLAAAIAAAAIASASAWDLEAASGRFGVIIVNNQYDPLPL
                     VPVTNTLGGALAISFSKGFFLSLQPALDLYWTNYKWYEGRAVPTESETGEGNNAFVLG
                     FIVDLPLTASFRFGERLGGAFGLGPAFVLRASFKNDTTAGVEEAMAGNLAAIASYFWS
                     DGRWFYPSASLRFDVYLQQNFTFAFGVRGLMPVYNAWTGNERIFDEGMLHVTMAMYVG
                     LE"
     gene            complement(2236..>2487)
                     /locus_tag="PLQ29_10160"
     CDS             complement(2236..>2487)
                     /locus_tag="PLQ29_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87051.1"
                     /translation="GQAAPAWGHLYGDRACVATDDWRVVLADGASGVIVGPSPVPPYP
                     GATVRDAVLLEDALILLWEAGSFPYLWASGLVILDAPRK"
BASE COUNT          420 a          816 c          866 g          385 t
ORIGIN      
        1 tctggggcgc tgccgcgctc ggcatcgccc tcaagccctt cctgacgggc aagatgaagt
       61 tcgtctccac tgtcgcctac gtgctgatgg gatggatcat cgtcttcgcg tggaagccgc
      121 ttgctgccgc cgtcgacgtc gcggccctac ggtacctcgt cgccggaggc ctggcctata
      181 cgatcggcgc cgccttctac ctgctcaaaa gacggtgctg gacccacccg gtatggcacc
      241 tgttcgtcgg tgccggcgcg acgctccact tcttcgcggc gctggcgctg ctacctcagg
      301 gctgacgcga gcctgggcgg cgaggtagcc tcggcttctt gagccagaga atcctcgacg
      361 ccgccgacgc taccgccagg ccaccggcga tggctccggc cagcgtcagc gtctcgaagc
      421 cgacgctcgc gctagcggcg gcgcggccgt tgaccgcctc gagcatggtg cggtacatgc
      481 ccgcgccggg cacgagcggt atgatggccg acaggatgaa cagggtggcc ggccgtttaa
      541 ggcaggacgc catggcctcc gcatagaggc cgaccgcgac gctcgcggcg aagtacgata
      601 gggtgtcgga gccggtggcc gccttgaagg ccaggtaggc gccccacgat acggcacccc
      661 cgagggcggt gaatggcagg tcgcggagcg gggccctgaa cagtaccccg aagccgaggg
      721 tggccaggaa ggcccaggcc agctggacgg cctgggcgct cacggcgtcg gtcctgagat
      781 cagcatggcc agctgcagcg ccaagccgac gccgatcgag atgccggccg ccgagatgaa
      841 cgcgtcggcc gcccgggcta tgccagccac caggtcgccg gctatcaagt cccgtatggc
      901 attgactatg agcacgccgg gcacgagtag catgatggag ccgatgatca tcgcgtcggt
      961 attgcccgcg acgcggaggg cctcggcgcc gatggcgacg gcggccgcga aggctccgcc
     1021 gacaatattg gagaagaaaa ccgacagctt catgccgccg agcgccgacg ttatcctgcc
     1081 tatgacgagg ccgatcccgg ccgagaccag cgcgtcgttc caggcgccgc cgaatagcag
     1141 gcagaagaag ccggtaacga aggcggcgcc gacggcgggt atcggccgcg ggtaggcgcc
     1201 gagcgcgtct atggcgcata gctcccgctc tatcccgtcc cgatcgagtc tgcccgatac
     1261 ggcgtcccgc gcgagcgcgt tgacgcgggt gatcttgtcc aggttcagct ggtgcttgcg
     1321 tatgcgcctg acgaccgccc tgccgcgccc gtaccggtcg tcgcaggacg ccatgaagcc
     1381 ggtcggcgtg gcgaaggcct ccggctcgag cgctccgagg gccgaggcca cggcggacat
     1441 gacttcctcg gcccggtagg tctcgccgcc ggactcgagc acgatgcggg cggcttcggc
     1501 agacagcctg agcgcgtcgt cggtattgtc ctcgtcgtcc gtcgtcattc cagccccacg
     1561 tacatcgcca tggtcacgtg cagcatgccc tcgtcgaaga tgcgctcgtt gccggtccag
     1621 gcgttgtaga cgggcatgag cccgcggacg ccgaaggcga aggtaaaatt ctgctgtagg
     1681 tagacgtcga aacgcaacga ggcggagggg tagaaccagc ggccgtcgga ccagaagtac
     1741 gaggctatag ccgcgaggtt gccggccatt gcttcctcga cgcccgccgt cgtgtcgttc
     1801 ttgaaggagg cgcgcagcac gaaggctggc ccgaggccga aggcgccgcc taggcgttcg
     1861 ccgaagcgga agctggcggt cagcggcaga tcgacgatga agccgagcac gaaggcgtta
     1921 ttgccctcgc cggtctccga ctcggttggt acggcgcggc cctcgtacca cttgtaattg
     1981 gtccagtaga gatcgagcgc cggctggagg ctcagaaaga agcccttgga gaacgatatg
     2041 gccagcgcac cgcccaaggt gttcgtgacc gggacgagcg gcagcgggtc gtactggttg
     2101 ttgacgatga tgacgccgaa gcggcccgac gccgcctcca ggtcccatgc gctcgcggag
     2161 gcgatcgcgg ccgctgcaat ggccgccgcc aagacgattc tcttcatcgt attgctacta
     2221 tagcgcgccg acggctcatt tcctaggggc gtcgaggatc accagaccgc tcgcccatag
     2281 gtacgggaag gagccggcct cccagagtag gatcagggcg tcttcgagca ggaccgcgtc
     2341 cctaacggtc gcgcccggat agggcggtac gggggatggg ccgacgataa cgcccgaggc
     2401 tccgtcggct agcacgacgc gccagtcgtc ggtggccacg caggcgcggt ctccgtatag
     2461 atggccccag gccggcgcgg cctggcc
//
LOCUS       DAOPHV010000412         2487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_59212_length_2487_cov_3.241776, whole genome shotgun sequence.
ACCESSION   DAOPHV010000412 DAOPHV010000000
VERSION     DAOPHV010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59212_length_2487_cov_3.241776"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PLQ29_10165"
     CDS             <1..394
                     /locus_tag="PLQ29_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87052.1"
                     /translation="GTARKVAASFDYIFHFVQLKNKSQKRLKGIYELGINRKEEKISI
                     RQICRYDHKSDNWQWEYIAGEDKIAIGEEENHEVTIGSRLYLSFGCSCQRGGKVSILH
                     YPSIVFLIELMKWHWMNVLINVGQFDCL"
     gene            complement(795..1169)
                     /locus_tag="PLQ29_10170"
     CDS             complement(795..1169)
                     /locus_tag="PLQ29_10170"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPG87053.1"
                     /translation="MSARRAITSAAALVALAVATLGSCALPDIRVTTPPFSGLPDGAY
                     RGYYDGGIVKAEVEATLAGGSLTAVTILRHDCGKGRPAEAIVDEVVAAQSLDVEVVAG
                     ATYSSKVILKAIEIALTDGRAF"
     gene            complement(1166..1642)
                     /locus_tag="PLQ29_10175"
     CDS             complement(1166..1642)
                     /locus_tag="PLQ29_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPG87054.1"
                     /translation="MKTLIAYATKRGATRDMAARIAGGLGGADIVDLDGPEAGRVDLS
                     AYGTVVIGASTYAGSLRKSAVTWAKAREAELASKKVALFEIGSAKDALAVETALPGLC
                     AVAVAVAKLGGEIRWKRLSLPERWIIKAIMKTEGDSSTIDDDAVEAFVEAVRKASL"
     gene            complement(1639..2253)
                     /locus_tag="PLQ29_10180"
     CDS             complement(1639..2253)
                     /locus_tag="PLQ29_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87055.1"
                     /translation="MKDRLTAATLKAIDSRRGAHGLSLRGIAKDAGCSHVNVYNYAPR
                     GLADLLWLAYTVGIEEYAKACFESVRNRGPGENYGEALARGMLAFATEREGMYRLLWF
                     DALEGEPEGEALAAIRRAQEGFLAETNAQLEDAGLSGMADAFAPSLEIMFAYLQGEVA
                     LFINGRSGSDRKSAAEAIVARSGRTWRILIEGARPNGEPGRSPV"
BASE COUNT          489 a          745 c          711 g          542 t
ORIGIN      
        1 tggaacggcg cgtaaagtag cggcaagctt cgactatatc tttcattttg tgcaattgaa
       61 gaataaaagc caaaagcggt taaaaggaat ctacgaattg ggaatcaacc ggaaagaaga
      121 aaaaatttca atcagacaaa tttgtagata tgatcacaag tcagataact ggcagtggga
      181 atacattgcg ggtgaagata aaatagcaat cggagaagaa gaaaatcatg aagtcactat
      241 aggttcccgc ctttatctct ccttcgggtg tagctgccaa aggggtggta aagtctccat
      301 attgcattat ccaagtattg ttttcctcat cgaactgatg aaatggcact ggatgaatgt
      361 actcattaac gttggccaat tcgactgctt gtagggcaac cttgaatgta gatggtgtta
      421 aactacagat tttatttccg atgcttgtat attcataata agaagatacg aacgatggag
      481 gagctgcatc cctttcagcg cttgatcgaa ggagtgctct aggggcggat gtccccgacc
      541 attgaatttt tgcgcttatt gtttgattgg gagattcctc gtctaaaggt tcttgacaag
      601 aggataacgc cacggtagaa gcaagtagta gcatcgcagt caacattgta agtctcagct
      661 tcttcattct cattcccttt attcactcgt gataaaaata aaactcatct ttataaccag
      721 tctagagtat aggaatgatt agtcaagttc tttatgcaac ttgtcggttt caagcctatc
      781 gtcgatctgg atcattagaa ggcacgacca tccgttaggg ctatttcgat ggccttgagt
      841 atgaccttgc tcgagtaggt ggcgccggcc acgacttcga cgtcgaggct ctgggccgcg
      901 acgacctcgt cgacgatggc ctcggccggc ctgcccttgc cgcagtcgtg cctaaggatc
      961 gtcacggccg tcagcgaccc gccggccagc gtcgcctcca cctccgcctt gacgatgccg
     1021 ccgtcgtagt agccgcggta ggcgccgtcc ggcaggccgg agaacggggg cgtcgttacc
     1081 cgtatgtcgg gtagcgcgca cgaaccgagc gtcgcgacgg cgagcgcgac cagcgcggcc
     1141 gccgatgtaa tcgcacggcg cgcgctcata gcgacgcctt ccgcactgcc tcgacgaagg
     1201 cctcgacggc gtcgtcgtcg atggtcgacg agtcgccctc ggtcttcatg atagccttga
     1261 tgatccagcg ctcgggaagc gacaggcgct tccagcgtat ctcgccgcct agcttcgcca
     1321 ccgcgacggc cacggcgcat agcccgggca gggcggtctc gacggcgagc gcgtccttgg
     1381 ccgagccgat ctcgaacagc gcgaccttct tggacgctag ctcggcctcg cgggccttgg
     1441 cccacgttac cgcggacttg cgcagcgagc cggcgtaggt cgacgcgcct atgacgaccg
     1501 tgccgtaggc cgataggtcg acccgcccgg cctcggggcc gtccaggtcg acgatatcgg
     1561 cccctccgag gcccccggct atgcgggcgg ccatgtctct cgtcgcgccc ctcttggtgg
     1621 cgtacgcgat caacgtcttc atactggact ccttccgggc tccccgttcg ggcgggcccc
     1681 ttcgatcaat atgcgccagg ttcgccccga tcgggcgacg atggcctcgg ccgcggactt
     1741 cctgtccgag ccggatctgc cgtttatgaa cagcgctacc tcgccctgga gataggcgaa
     1801 catgatctcc agcgacggcg cgaacgcgtc ggccattccc gacaacccgg cgtcctcgag
     1861 ctgggcgttc gtctccgcga ggaagccctc ctgcgcgcgc cgtatcgccg cgagcgcctc
     1921 gccctccggc tcgccctcga gcgcgtcgaa ccagaggagc cgatacatgc cctctcgctc
     1981 ggtcgcgaag gcgagcatgc cgcgggctag ggcctcgcca tagttctcgc cgggtccgcg
     2041 gttccgtacg ctctcgaagc aggccttggc gtactcctcg atgcctaccg tgtaggccag
     2101 ccagagcagg tcggcaaggc cgcgcggcgc gtagttgtag acgttgacgt gcgagcaccc
     2161 ggcgtccttg gctatcccgc gcaggctaag gccatgcgcg ccccttcgtg aatcgatcgc
     2221 cttgagcgtc gccgccgtca ggcggtcttt cattgattcg ttcattacca ctggtaactt
     2281 accaatggta atgctctctg tcaagtgcta cgctacgaag ctagtcgaag cgctcgagcg
     2341 ccgctcccgc gatcctcgag ttcttgactt ttttccccgc tttacggcat tctaccggcg
     2401 ccccggatca cccgatcccg ggcgcctttt tcgcgggaat agctcagtgg tagagcgcca
     2461 ccttgccaag gtggatgtcg cgggtcc
//
LOCUS       DAOPHV010000413         2483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_59324_length_2483_cov_4.326606, whole genome shotgun sequence.
ACCESSION   DAOPHV010000413 DAOPHV010000000
VERSION     DAOPHV010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59324_length_2483_cov_4.326606"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLQ29_10185"
     CDS             complement(<1..392)
                     /locus_tag="PLQ29_10185"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPG87056.1"
                     /translation="MGDHITSGRRNEPVRVALLIALAAVVFVAYSSHLFYLQVIRGGE
                     YQKKAVTIARKIERLPAQRGEIYDRNYNLPLVLNVDSFALVVVPAEIPADLRDTVFAK
                     LASVLEEPIDEIRRRIPPSYYRLFQQIEV"
     gene            complement(394..897)
                     /gene="mreD"
                     /locus_tag="PLQ29_10190"
     CDS             complement(394..897)
                     /gene="mreD"
                     /locus_tag="PLQ29_10190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPG87057.1"
                     /translation="MIRRWLASLALVSLLIVVQSTWLGVIAVYSVLPDLSLIAVVYIA
                     FKSPNLQGQGVGFVAGLLQDGISAAPFGLNAFVKTAVAWLANLLSGKFYIDRLLMPAL
                     FGFSMTLAKAAYLAVLASLFSGKITGYDFLGSTLWIEAAYNAVVAPFMFMLLGFLDRF
                     IVPRDAR"
     gene            complement(894..1766)
                     /gene="mreC"
                     /locus_tag="PLQ29_10195"
     CDS             complement(894..1766)
                     /gene="mreC"
                     /locus_tag="PLQ29_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPG87058.1"
                     /translation="MAARERRAPRRAIGSALFMAVSLITLVVSTRSLAGMPERVGLSV
                     LSFVQRGFDSVGTFVSDTVNSIAELRRLEKNYDDLLARLETLSTVERSYTDLKRENER
                     LREQLGFKNDSAYVAISAKIIARDPENLYSTFVLDKGSTNGIAKNQAVVAYQDGAEGL
                     VGRVIEVGRTSCVVSPIYDSSAYVAVKLERSRYDGIAVGSGSDEAPIIVRYVKKRAKD
                     EIQYGDLVVTSGMQSLYPPGIGVGRVTMMRDLDYLTSLELGMEPLIDFGRIEYVFVVQ
                     QNRPDSAGSAESDR"
     gene            complement(1772..>2483)
                     /gene="mreB"
                     /locus_tag="PLQ29_10200"
     CDS             complement(1772..>2483)
                     /gene="mreB"
                     /locus_tag="PLQ29_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HPG87059.1"
                     /translation="TCITEVERRAVVEAAYKAGAREVEVLPESLAAAIGSDIPIHEPA
                     GHMICDIGGGTTEISVISLKDLVVTNAIRIGGDEFDEAIIKHIRAAHNLIIGEQTAER
                     LKIEIGNASPDKVIEKVEIKGTDAITGLPRRLEIDSVEVREALQEPVNQIVEEIKKTL
                     GQTPPELAADIVERGIVMAGGGSLLKGLPRLIAKETGVPVILAERPLECVAIGAGKYF
                     EAIADVDVAFGSAASMNL"
BASE COUNT          457 a          753 c          798 g          475 t
ORIGIN      
        1 acctctatct gctggaacag cctgtagtac gagggcggga tacgccgcct gatctcgtct
       61 atcggctctt cgagcacgga cgcgagcttg gcgaagacgg tatcgcgcag gtccgccgga
      121 atctcggcgg gaacgacgac gagcgcgaac gagtcgacgt tcaacacgag cggcaggttg
      181 tagttgcggt cgtatatctc gccgcgctgg gccggcaagc gctcgatctt cctggctatc
      241 gtgacggcct tcttctggta ctcgccgccg cgtatgacct gcaggtagaa gaggtgcgac
      301 gagtacgcga cgaacacgac ggccgcgagg gctatcagca acgctacgcg gacgggctcg
      361 ttccggcggc ccgaggtgat atggtcgccc atactatcgg gcgtcccggg gcacgatgaa
      421 gcggtcgagg aatccgagta gcatgaacat gaacggcgcg acaacagcgt tataggccgc
      481 ctcgatccag agagtcgatc cgaggaaatc gtaaccggtt atcttgcccg agaacagcga
      541 tgctaggacc gcgaggtacg ccgccttggc gagggtcatc gagaaaccga agagagccgg
      601 catgagcagg cggtctatat agaatttgcc cgatagcaga ttggccagcc aggccacggc
      661 cgttttcacg aacgcgttga gaccgaacgg cgcggcgctt atgccgtcct ggaggaggcc
      721 ggcgacgaag ccgacgccct ggccctgcag gttcgggctc ttgaaggcta tatatacgac
      781 ggcgatcaac gacaagtcgg gcagtaccga gtagacggct atgaccccca gccaggtcga
      841 ctgcacgact atgaggagtg agacaagggc gagcgaagcg agccaccttc gtatcatcta
      901 tcgctctccg cgcttcccgc gctatcgggt ctgttctgct ggacgacgaa tacgtactcg
      961 atgcggccga agtcgatcag gggctccata ccgagctcga gcgacgtcag gtagtcgagg
     1021 tcccgcatca tcgtgacccg gccgacgccg atgcccggcg ggtacaggga ttgcatgccg
     1081 ctcgtaacta ccaggtcacc gtactgtatc tcgtccttag cgcgcttctt aacgtagcgt
     1141 acgatgatcg gcgcctcgtc cgagccggag cctacggcta taccgtcgta gcgcgatcgc
     1201 tccagcttga cggccacgta cgcggagctg tcgtagatgg gagagacgac acagctggtg
     1261 cgccccacct ctatgaccct gccaacgagg ccttcggcgc cgtcctggta ggctacgacc
     1321 gcctggttct tggctatgcc gttcgtagag cccttatcga gcacgaaggt tgagtacagg
     1381 ttttcaggat cgcgcgcgat tatcttggcc gaaatggcta cgtacgcgga gtcgttcttg
     1441 aagccgagct gctcgcgcag tctctcgttc tcgcgtttca ggtccgtata actgcgctcg
     1501 accgtgctga gcgtctcgag cctggccagg aggtcgtcgt agttcttctc gagtcggcgt
     1561 agctcggcga tgctgttgac cgtatcggag acgaacgttc cgacgctgtc gaagccgcgc
     1621 tgcacaaagg agagcaccga taagccaacg cgctccggca tacccgcgag gctacgagtc
     1681 gagacgacga gcgtgataag cgagacggcc atgaagagcg cggaaccgat cgcgcgccgc
     1741 ggggctcgcc gttcgcgtgc tgccatgccc gttataggtt catcgaagcg gcggagccga
     1801 aggcgacgtc tacgtcggcg atggcttcga agtacttgcc tgcgcctatg gcaacgcact
     1861 cgagggggcg ttcggccagt atgaccggga cgccggtctc cttggctatc aggcgcggca
     1921 gtcccttgag gagcgatccg ccgccggcca ttacgatgcc ccgctcgacg atgtcggcgg
     1981 ccagctcggg cggggtctgg ccgagcgtct tcttgatttc ctcgacgatc tggttgacag
     2041 gctcctggag cgcctcgcgc acctcgaccg agtcgatctc gagcctacga ggcaggccgg
     2101 ttatggcgtc ggtacccttg atctcgacct tctcgataac cttgtcgggg ctggcgttgc
     2161 ctatctcgat cttgagcctc tcggccgtct gctcgccgat gatcaggttg tgagcggcgc
     2221 ggatatgctt gatgatggcc tcgtcgaatt cgtcaccgcc gatgcgtatc gcgttcgtca
     2281 ccacgagatc cttgagggag atgaccgata tctcggtggt accgccgccg atgtcgcata
     2341 tcatatggcc ggccggctcg tgtatcggaa tgtccgagcc gatggcggcc gcaagcgact
     2401 cgggcagaac ctcgacctcg cgagcgccgg ccttgtaggc cgcctcgacg accgcgcgtc
     2461 gctccacctc ggtgatgcag gtc
//
LOCUS       DAOPHV010000414         2476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_59571_length_2476_cov_4.828170, whole genome shotgun sequence.
ACCESSION   DAOPHV010000414 DAOPHV010000000
VERSION     DAOPHV010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59571_length_2476_cov_4.828170"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="PLQ29_10205"
     CDS             <1..1291
                     /locus_tag="PLQ29_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87060.1"
                     /translation="REGRLADAERALLAGAAIDQLDDRFVELSDRIRSEAARARAATI
                     EDVDEAPDSGADAVPWRVANRFYLEAVQARDEGRLFDAHYLAKRSAAIYSNDVNTRRL
                     VAETWSALQALGPSLEEKAAAAFYRRKLEGYSRYQEGDYLEAFRIYSELAEEAPDDAD
                     VTEYLGRSALGLSDISFFIEEDERAFSRSDERPFSVTLSGPRPARLSASRAAASEGAV
                     YFRDLYLALGGASPLEVRAPFARLHGDRLILRAVDRSTPDLVWEPEYVRGPSSGSGQD
                     DPGYAIIVPFTHEDALMAIRLSGSPGDIPLAVLATGIDDAERIGFASWPLKVELAERC
                     SYPFAVLMLALLGVGLGVRFRPTEPIGAVSRYLTSPLLVALAVPPLRLAAELSAMATR
                     TLAALVPSAWFLPAWIGVLSLCVVASLLVSARIASRR"
     gene            complement(1343..>2476)
                     /gene="murA"
                     /locus_tag="PLQ29_10210"
     CDS             complement(1343..>2476)
                     /gene="murA"
                     /locus_tag="PLQ29_10210"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPG87061.1"
                     /translation="VFVMLDICRSLGASVDRLGPNDWTIDGRAVGQPEVPASLAARVR
                     ASILFAGPLLARFGRVTLPPPGGDVIGRRRLDTHFLALEELGARIEVDGSFQFTANKL
                     KGADIFLDEASVTATENAVMAASIAEGRTVIRNAASEPHVQDVCRLLLAMGASISGIG
                     SNILTIDGAAALHGADFTIGPDFMEVGSFIGLAAVTRGDIVIEGVAPDDLRMMKLAFG
                     KLGVTWSLDGDALSVHSGQGMRVVPDLGGQIPKIDDAPWPGFPPDLTSIMTVVATQAE
                     GTVLIHEKMFESRMFWVDKLIGMGARIVLCDPHRAVVSGPARLRGDSLTSPDVRAGMA
                     LVIAALCAEGTSVIKNVYQIERGYERIAERLQALGAKVERLKE"
BASE COUNT          381 a          825 c          852 g          418 t
ORIGIN      
        1 ccgggagggg cgcctggctg acgccgaacg ggcgctcctg gccggcgccg cgatcgacca
       61 gctcgacgat cgcttcgtcg agctgagcga caggatcagg tcggaagccg cgcgcgccag
      121 ggcggcgacc atcgaggatg tggacgaggc ccctgactcc ggcgccgacg ccgtgccgtg
      181 gcgggttgcc aaccgcttct acttggaagc agtacaggcc agggatgagg gacggctctt
      241 cgacgcgcac tacctggcca agcggtcggc ggccatctac tcgaacgacg tcaacacgag
      301 acgactggtc gccgagacct ggtcggcgct acaggcgctc gggccatcgc tcgaggaaaa
      361 ggcggccgcc gcgttctatc gtcgcaagct cgagggttac tcgcgctatc aagaaggcga
      421 ctacctggag gctttccgca tctattcgga gctagccgag gaggctccgg acgacgcgga
      481 tgtaaccgag tacctaggac gcagcgccct cgggctatcg gatatttcgt tcttcatcga
      541 ggaggacgag cgggcgttct ccaggtccga tgagaggccc ttttccgtga ccctttccgg
      601 tccgcgcccg gcccgcttga gcgcgtcgag agcggccgcc tcggagggcg ccgtctactt
      661 ccgagacctg tacctggcgc tcggaggggc ttcgccgctc gaggtgcgtg cgccgttcgc
      721 gagactccat ggcgataggc tcatcctgag ggccgtggat cggtcgacgc ccgacctggt
      781 ctgggagccc gagtacgtgc gcgggccgtc gagcggctcc ggccaggacg atccgggcta
      841 cgcgatcatc gtcccgttca cccacgaaga cgcgctgatg gccatccgcc tctccggatc
      901 gccgggcgac attccgctgg ccgtgctcgc gaccggcatc gatgacgccg agcggattgg
      961 gttcgcttca tggcctctca aggtagagct ggccgagcgt tgctcctatc cgttcgccgt
     1021 cctgatgttg gcgcttctgg gcgtcgggct gggcgtgcgc ttcaggccga ccgaaccgat
     1081 aggggcggtg agccgctacc tgacctcgcc gttgctcgtg gcgctcgccg tacctccgct
     1141 acggctggcg gcggagctgt cggccatggc cacgaggacc ttggccgcgc tggtcccgag
     1201 cgcttggttc ctgccagcct ggataggcgt cctgtcgctg tgcgtggtgg cgagcctcct
     1261 cgtatcggcg cgcatagcct cgcgccgcta ggatgaccgc ctcgctgccg cctggcggcc
     1321 gcctggcggc aacgaaacgc gactactcct tgaggcgctc caccttcgcc ccgagcgcct
     1381 ggagcctctc ggcgatgcgc tcgtagccgc gctcgatctg gtaaacgttc ttgatgacgc
     1441 tcgtgccctc ggcgcagagc gccgcgatga cgagggccat accggcgcgc acgtcgggcg
     1501 aggtcaacga gtcgccgcgc aggcgcgccg gcccggagac gacggcgcgg tgcggatcgc
     1561 agagcacgat cctggctccc atgcctatca gcttgtcgac ccagaacatg cgcgactcga
     1621 acattttctc atggatcagt acggtacctt cggcctgagt cgccacgacc gtcatgatcg
     1681 aggtgagatc cggcgggaag cccggccacg gcgcgtcgtc gatcttgggt atctgtccgc
     1741 cgaggtcggg taccacgcgc atgccctggc ccgagtgcac cgacagcgcg tcgccgtcca
     1801 ggctccaggt tacgcccagc tttccgaagg ccagcttcat catgcgcagg tcgtcgggag
     1861 cgacgccctc gatgacgatg tcgcctctgg tcacggcggc tagcccgatg aacgagccga
     1921 cctccataaa atccggcccg atcgtgaagt cggcgccgtg cagggcggcg gctccgtcta
     1981 tcgtcaggat gttcgagccg atgccggaga tagacgcgcc catcgccagg aggagcctgc
     2041 agacatcctg tacgtgcggc tcgctcgcgg cgttgcgtat cacggtgcgg ccctcggcta
     2101 tggaggcggc catgacggcg ttctcggtgg ccgtgacgga ggcttcgtcg aggaagatgt
     2161 cagcgccttt gagcttgttg gcggtgaact ggaacgagcc gtccacctcg atgcgcgccc
     2221 cgagctcctc gagcgccagg aaatgcgtgt cgaggcgcct gcgacctatg acgtcgcctc
     2281 ccggaggcgg cagggtcacc ctgccgaacc tggccaggag ggggccggcg aacaggatgg
     2341 aggcccggac tctcgcggcg aggctcgcag gcacctcggg ctggccgacg gcgcgaccgt
     2401 ctatcgtcca gtcgttagga ccgagcctat cgacggaggc gccaagggac ctgcagatgt
     2461 cgagcatcac gaagac
//
LOCUS       DAOPHV010000415         2474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_59643_length_2474_cov_4.469202, whole genome shotgun sequence.
ACCESSION   DAOPHV010000415 DAOPHV010000000
VERSION     DAOPHV010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59643_length_2474_cov_4.469202"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..1932)
                     /gene="ade"
                     /locus_tag="PLQ29_10215"
     CDS             complement(196..1932)
                     /gene="ade"
                     /locus_tag="PLQ29_10215"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HPG87062.1"
                     /translation="MLKTDYIDVARGRIPADLNLTDCRVVDFFRGRIVDRAVVSIYKG
                     FIVGIDDGLIAKSTMSLGGRYLCPGLIDAHVHIESSLLSPAEFARVITPRGTTAIVAD
                     PHEIANVLGHDGVNWMLKASEGLPLDVYVMVPSCVPATHFDTAGATLFASDLARFARD
                     ERVLGLGELMNYPGLLAGDPRVLDKLSLFWEAGKAIDGHAPGLSGRDLSAYALSGIRS
                     DHEATTPEEAYEKLGKGMWIMARMGSSARDLENLIPAMDGESVRRMMLCTDDRHPNDI
                     VSEGHIDAAVRALVSGGVPPIDALRMACHNPATYYGLRRSGAIAPGYAADLVAFSSLE
                     DFRAELVLKAGAVVAEGGVPTAAYERLGGSAVVPPLRDSVNIKWLEEKDFRIPDEGGD
                     ARVIESTPGSIITGASFERPTVRDGLCVSDIERDIVKIFVIERHTGSGNIGKGFIRGL
                     GLKRGAVGSTVSHDSHNMIIAGVDDASIFKAARRLNAMKGGYVITDGDEVLAELALPV
                     AGLMSDRPAVEVIEQLASFEDFFRMEGMPGASPLMTLSFMALPVIPALKITDKGLVDV
                     NSFEQVPLFRMP"
     gene            2195..>2474
                     /locus_tag="PLQ29_10220"
     CDS             2195..>2474
                     /locus_tag="PLQ29_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HPG87063.1"
                     /translation="MEQLFKLKQHGTTVRTEIMAGITTFMTMAYILAVNPGILSATGM
                     PAGGVFTATALASMIATLLMAFMANLPIALAPGMGLNAFFAFTVVLTMG"
BASE COUNT          463 a          740 c          803 g          468 t
ORIGIN      
        1 gggatgtaag gagtccgcct gaagtcgcgg gtaaccccgc gatcgcctgg gccccgagac
       61 gtgtacgagc attatggtga gaagctagtg gtgtataata actgtagtcc aaggacgacc
      121 cgggagcaat ggcaaggcat cgttcctgaa cgcgggtgga gtaccggagg cgccgccgga
      181 ttcggccgga tgccgtcaag gcattctaaa aagtggtacc tgttcgaacg agttaacgtc
      241 gacgagcccc ttgtcggtta tcttgagggc cgggataacc ggtagggcca tgaaagagag
      301 ggtcatgagg ggtgaggctc ccggcatgcc ctccatcctg aagaaatctt cgaatgaggc
      361 gagttgttct atgacctcga ccgcaggacg gtcgctcatg agtccggcga ccggtagggc
      421 cagctcggcc agcacctcgt ctccgtcggt gatgacatag ccgcccttca tcgcgttgag
      481 cctgcgcgcc gccttgaaga tcgaggcgtc atcgacgccg gcaatgatca tgttgtgcga
      541 gtcgtggctg accgtcgatc caacggctcc gcgcttgagg ccgagccccc tgatgaagcc
      601 cttgcctatg ttcccggagc cggtatgacg ctcgatcacg aatatcttga cgatgtcgcg
      661 ctcgatgtcc gatacgcata ggccgtcgcg cacggtcggg cgctcgaacg acgcgccggt
      721 tatgatcgat ccgggcgtcg actcgatgac gcgggcatcg ccgccctcgt ccggaatcct
      781 aaaatccttt tcctcgagcc acttgatatt gaccgagtcg cgcaggggcg ggactaccgc
      841 ggagccgccg agccgctcgt aggctgccgt cggaacgccg ccctcggcta cgaccgcgcc
      901 ggccttgagc acgagctcgg ccctgaaatc ctcgaggctg gagaacgcga cgaggtcggc
      961 ggcgtagccc ggggctatgg cgccggagcg cctgaggccg taataggtag cggggttgtg
     1021 gcaggccata cgcaacgcgt cgatcggcgg cacgccgccc gagaccagcg cccgtacggc
     1081 ggcgtcgata tgcccctcgg acactatgtc gttcgggtgc ctgtcgtcgg tgcagagcat
     1141 catgcgccgt acgctctctc cgtccatcgc ggggatcagg ttctcgaggt cgcgggcgga
     1201 cgagcccatg cgggccatga tccacatgcc cttcccaagc ttctcgtagg cttcctcggg
     1261 tgtcgtggcc tcgtggtcgc tcctgatgcc tgataatgcg taggccgaca ggtcgcggcc
     1321 ggagaggccg ggcgcgtggc cgtcgatggc cttgcccgct tcccagaata gcgacagttt
     1381 atcgaggacc cttgggtcgc cggccagcag accgggatag ttcatcagct cgccgagtcc
     1441 gagtacgcgc tcgtctcgcg cgaagcgggc gaggtcggac gcgaacagcg tggcgccggc
     1501 cgtgtcgaag tgcgtggcgg gcacgcagga cggcaccatg acgtagacgt cgagggggag
     1561 gccctccgag gccttgagca tccagttgac gccatcgtgc ccgaggacgt tggctatctc
     1621 gtgcggatcc gccacgatgg cggtcgtgcc ccgtggcgtg atcacgcggg cgaattctgc
     1681 cggcgagagc aggctcgact cgatatgcac gtgggcatcg atcaggccgg ggcagaggta
     1741 gcggcctccg aggctcatcg tcgacttggc tataaggccg tcgtcgatcc cgactataaa
     1801 gcccttgtat atcgatacca cggccctgtc gacgatgcgg ccgcggaaga aatccacgac
     1861 gcggcaatcg gtcaggttga ggtcggcggg tatacgtccc cgggcaacgt cgatgtagtc
     1921 ggtcttaagc atagttggta gcctgtcccg tgaatgcggg tgttcagtat tgttacagca
     1981 aatcccggac ttgtcaaatc attatttagc gcataggcgg gatgcggtca agcgcggtgc
     2041 ggtcaggctc gtcggaacga accaaaacgt agtaaacgcc actaatcgcc acataatgaa
     2101 taaaaaaaaa tctgttgcgg tttttatcat tgcgtcgtac gctgatgccg attatgccgt
     2161 tcgaacggcg aaacaaacca aaggaggctg aaaggtggaa cagcttttca agcttaagca
     2221 acacggcacc acggtgcgga ccgagatcat ggcgggtatc accacgttca tgacgatggc
     2281 gtacatcctg gcggtcaacc ccggtatcct cagcgctacc ggcatgccgg ccggcggcgt
     2341 gttcacggcg acggccctcg cgtcgatgat cgctaccctg ctgatggcct tcatggccaa
     2401 cctgccgatc gccctcgcgc ccggcatggg cctgaacgcg ttcttcgcgt tcaccgtcgt
     2461 cctcacgatg ggct
//
LOCUS       DAOPHV010000416         2474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_59668_length_2474_cov_2.527904, whole genome shotgun sequence.
ACCESSION   DAOPHV010000416 DAOPHV010000000
VERSION     DAOPHV010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59668_length_2474_cov_2.527904"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="PLQ29_10225"
     CDS             <1..1072
                     /locus_tag="PLQ29_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87064.1"
                     /translation="VARAIDPANAAVTAYLAKLVTNTTKFKIQLESYVSYLNPAFLGI
                     IRQDSIHLVTGMPVWPVYYMPLVEGLNYTSYGDDRYVSETVSASQFGYSLPLGDAFGV
                     RLSGHFAALDVRTRVGSPPISDGSGGQWSNNNSARGAIGGGLDAGVRLGDAFAIGAGF
                     AYFARSDQDTSPIGPIVKNGGTTWAADLGFLVRNADESLVWDTRASYIAETQDLVDPL
                     TLALTGETQPFPLGIENNVTFSLLGRKLFIILKNSESIALGDTMISSSLMPAAEYFLL
                     PWLSLRCGVEGTMLVMDGLEPQFGLGGMGGLTLRLVKAGLDLDLNVSYRMRPSSAVPG
                     YLYPDFLILLNANWNGVFKSQR"
     gene            1626..2174
                     /locus_tag="PLQ29_10230"
     CDS             1626..2174
                     /locus_tag="PLQ29_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPG87065.1"
                     /translation="MKTFVCSVCGYVYVGEEAPAQCPQCKAPKVKFVEKAAVSTGAFA
                     DEHRIGVAKGLDAEVVQGLRDNFMGECTEVGMYLAMSRQADREGYPEIAEAYRRYAFE
                     EADHAARFAELLGEVVHADTKLNVSLRAEAEYGACEGKLMLAKRAKELGYDAIHDTVH
                     EMCKDEARHGAGFRGLLARYFK"
     gene            2224..>2474
                     /locus_tag="PLQ29_10235"
     CDS             2224..>2474
                     /locus_tag="PLQ29_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPG87066.1"
                     /translation="MDTKGFLRSHGIQPSQQRLRIYEALAAVKSHPDAETIYKKLIGE
                     LPTLSRTTVFTTLDLFVRASLAQRLAITGTELRYDADTSR"
BASE COUNT          494 a          779 c          728 g          473 t
ORIGIN      
        1 cgtggcccgg gccattgacc ccgccaacgc ggccgtcaca gcctacctag ccaagctcgt
       61 caccaacacg accaagttca agatacagct cgagtcctac gtgtcctatc ttaatccggc
      121 cttcctcggc atcatccggc aggattccat tcacctcgtg accggtatgc cagtctggcc
      181 cgtctattat atgcccctcg tcgaaggact caactacacg agctacggcg acgatcgcta
      241 cgtctctgag acggtaagtg cctcgcagtt cggctacagc cttcccctgg gcgacgcctt
      301 cggtgtgcgc ctatcgggcc atttcgcggc cctcgacgta cggaccagag tgggatcccc
      361 gccgataagc gatggtagcg gcggtcaatg gagcaataac aattccgcgc gaggcgccat
      421 cggcggcggc ctggacgcgg gcgtacgcct gggagacgcc ttcgccatag gcgcgggctt
      481 cgcgtacttc gcccgctcag accaggatac gagccccata ggacccatcg tcaagaacgg
      541 cggcacgacc tgggcagccg atctcggctt cctcgtccgc aatgccgacg agagcctggt
      601 atgggacacg cgtgcgagct atatagcgga aacacaagac ctagtcgatc ccctgacgct
      661 cgccctgacc ggggagaccc agcccttccc cttgggcata gagaataacg tgaccttctc
      721 actgctcggg cgcaagcttt ttataatact aaaaaactca gagtctatag cgctcggcga
      781 taccatgatc tcgagttccc tcatgccggc cgccgagtat ttcctcctgc catggctttc
      841 cctccgctgc ggtgtggagg gtaccatgct cgtcatggac ggcctcgagc cgcagttcgg
      901 cctcggcggc atgggcggac tcacacttag gctcgtgaag gcgggcttgg atctggatct
      961 caacgtgtcc taccgtatgc gcccctcgag cgccgtgccg ggctacctgt accctgactt
     1021 cctcatcctg ctcaacgcca attggaacgg cgtatttaaa agtcagcggt gattggtgtc
     1081 gcgtcgcctg gtctgctccc gctccgacgg gagtaggctg gcgaaccggc caaggtgtac
     1141 cgatgcgtgc gctagagcga caccgtctag agcatacccg gcggcagaac cccctgcatc
     1201 ccctgcctag cgcaacgggg gtatgggctt cgtcacaaac attattgagg gcggtgctac
     1261 cgacgcgctc gacatctagc gcaaggtata accatcgctg gtgagcgtgg ctacgacgtc
     1321 tgggtcaagt acctcaacgt gcccggcacc gaggtgacga aaattcgccc gaccaagagc
     1381 cggggggccc catcgaagct gctgtagctt gaagtttttt gatggccgca tacgcaacct
     1441 cacgtcaaga cttcaaaata agttctcgcg taatcagcag tcacttggtt tctttgggaa
     1501 tagagtgcta tggccgccgt gcgccggcta gctggtcgac gctcagtcac catggagcga
     1561 ggggcttgat attctgtggt aattgctata ctgtaataag tacaaatatt tccatgggag
     1621 atagtatgaa gacattcgtc tgttcggtct gcggctacgt ttacgtcggc gaagaggccc
     1681 ccgcccagtg ccctcagtgc aaggcgccca aggtgaagtt cgtcgagaag gccgccgtat
     1741 ccaccggcgc gttcgcagac gagcaccgca tcggcgtggc caaggggctt gatgccgagg
     1801 tcgttcaggg ccttcgtgat aacttcatgg gcgagtgcac cgaggtcggc atgtacctgg
     1861 ccatgagccg ccaggccgat cgcgaaggct atcccgagat agccgaggcc tatcgccgct
     1921 acgccttcga ggaagccgac cacgcggctc gcttcgccga gcttttgggc gaggtcgtgc
     1981 acgccgacac caagctgaac gtatcgctac gcgccgaggc cgagtacggc gcctgcgagg
     2041 gcaagctcat gctggccaag cgcgccaaag agctgggtta tgacgctata cacgatacgg
     2101 tacacgagat gtgcaaggac gaggctcgcc acggcgccgg cttccgcggg ctgctcgcga
     2161 gatatttcaa gtaggtacgc ggcgggcggc tgcacgccgc ccgcactatc ataaggagct
     2221 aggatggata caaaaggctt cctgcgctcg cacggcatac agccatccca acagcgcctg
     2281 cgtatctacg aggcgctcgc cgcggtgaag agccatcccg acgccgagac catctataag
     2341 aagctgatag gggagctacc gacgctatcg aggaccacgg tgttcaccac gctcgacctc
     2401 ttcgtccgcg cgagcctggc ccagcgcctg gccataaccg gtaccgagct gcgctacgat
     2461 gccgatacct cgcg
//
LOCUS       DAOPHV010000417         2470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_59785_length_2470_cov_3.491511, whole genome shotgun sequence.
ACCESSION   DAOPHV010000417 DAOPHV010000000
VERSION     DAOPHV010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59785_length_2470_cov_3.491511"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PLQ29_10240"
     CDS             <1..841
                     /locus_tag="PLQ29_10240"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015713050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPG87067.1"
                     /translation="KIAASERVEPKHIDSLSQDLVQLVREKEAVVVSAILSSEFSGYD
                     MARAGINCAILSVVVGQTMKLPPHRLVHLASGALLHDVGMLRVPQEIIKKKGALSPEE
                     AQKMRAHPLLTYRIVIKELQYPDDVGLVGLQHHERWDGEGYPRRTSGQGIDLLARIVS
                     VADAFEAMVSQKPYRNSMIGYAAMKNLLSDNSRRFDPDVLKAFVRSMGIYPIGSIVVL
                     NNAAIARVVDTHADAPLRPRLKIIVDEFGKHFKDDSGDTIDLLAEKSLFIARAVDPRE
                     LSA"
     gene            844..1230
                     /locus_tag="PLQ29_10245"
     CDS             844..1230
                     /locus_tag="PLQ29_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPG87068.1"
                     /translation="MSTPDRRGTPDSRSAKGRAAEGIVSASLLASGWDVVARNWKRGR
                     GELDIVAFRDGVLAVVEVKDIDAFGLESVEASVGGRKRARIVETSKLFIHSHREFNSA
                     AVRYDVATVAAGALADYFENAFSERT"
     gene            1227..1391
                     /locus_tag="PLQ29_10250"
     CDS             1227..1391
                     /locus_tag="PLQ29_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87069.1"
                     /translation="MSSETNREEHAERLEELKAMVHDESYMSGAILRIANVLSDEIMD
                     GIGVSYGGWK"
     gene            1453..1824
                     /locus_tag="PLQ29_10255"
     CDS             1453..1824
                     /locus_tag="PLQ29_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87070.1"
                     /translation="METTGVQDGPVAAEKPEEQKSIFSDQPPVVCSICGKQIFDLSSA
                     LAGRDGGQPVHFDCALASVSEGERLEPTERIAYIGRGSFAVVEYRDKSMTAFTVKRRL
                     QWEKEGEKYDWRRAIQQRIGL"
     gene            1893..>2470
                     /locus_tag="PLQ29_10260"
     CDS             1893..>2470
                     /locus_tag="PLQ29_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPG87071.1"
                     /translation="MAEASYTVLVVDDSGANRAFLSSILEEDGYRVAQAADGEAGLVA
                     AATERPVLILLDVVMPRLDGFETLKRLKADPATRRISVIMLTSLGDQESKLKAFDYGA
                     VDYIIKSANEAEVRARVRVHVQLALANEELIEARAASLRQISEAQRSLLVKPTELPEA
                     RFSVYYRSLHEAGGDFYDVVPVSDGVYFYLIA"
BASE COUNT          484 a          744 c          810 g          432 t
ORIGIN      
        1 taagatcgcc gcgagcgaac gcgtggaacc caagcatata gactccctgt cgcaggatct
       61 cgttcagctg gtacgcgaga aggaggccgt ggtcgtttcg gctatcctgt cctcggagtt
      121 ctcgggttac gatatggcca gggcgggcat caattgcgcc atcctgtcgg tcgtggtcgg
      181 ccagaccatg aagctgccgc cccataggct cgtgcacctg gcgtccggcg ccctcctgca
      241 cgacgtcggc atgctgcgcg tgcctcagga gatcataaag aagaaaggcg cgctcagccc
      301 cgaggaggcg cagaagatgc gcgcccatcc gctcctgacc taccgcatcg tcatcaagga
      361 gctgcagtat ccggacgacg tcggcctggt cggccttcag catcacgagc gatgggacgg
      421 agagggatac ccgcgccgga cgagtggcca gggcatcgat ctgctcgctc gcatcgtgtc
      481 ggtcgccgac gctttcgagg ccatggtcag ccagaagccg taccggaatt cgatgatagg
      541 ctacgccgcg atgaagaatc tgctgtcgga caactcgcgg cgtttcgacc ccgacgtgct
      601 caaggcgttc gtccgtagca tgggcatcta tcctatcggc tccatagtcg tgctgaacaa
      661 cgccgccatc gcgcgggtcg tcgatactca cgccgacgcg ccgctacgtc cgaggctcaa
      721 gatcatcgtc gacgaattcg gtaagcactt caaggacgac tcgggcgata cgatagacct
      781 gcttgcggag aaatcgctct tcatagccag ggccgtcgac ccgcgcgagc tgtcggctta
      841 ggcatgagca cgcccgatag gcgtggcacg cccgatagcc gtagcgcgaa agggcgcgcg
      901 gcggagggga tcgtatcggc gtcgctactg gcgagcggct gggacgtggt ggcgcgtaac
      961 tggaagcgcg gacgaggcga gctcgatatc gtcgccttcc gggacggggt gctcgccgtc
     1021 gtcgaggtca aggatatcga cgctttcggc ctcgagagcg tcgaggcttc ggtcggcggg
     1081 agaaaacgag ccaggatcgt cgaaacgtct aaactcttca tccactccca tcgagaattt
     1141 aatagcgcgg ccgtccgtta cgacgtagcc accgtggcgg ccggcgcctt ggccgattac
     1201 ttcgaaaacg ctttctcgga gcgtacatga gtagcgaaac gaacagggaa gaacacgccg
     1261 aacggcttga agaactcaag gccatggtgc atgatgagtc ctacatgagc ggggccatac
     1321 ttcgtatagc caacgtgctg agcgatgaaa tcatggacgg tatcggagta agctatggcg
     1381 gatggaaata acggctcgaa gcgacggttc ggcaggagag ggcgggggcg cggaagatcc
     1441 ggcgctaagg gcgtggagac taccggggtg caggacgggc cagtcgcggc cgagaagccg
     1501 gaggaacaga agtctatctt ctccgaccag cctccggtcg tatgctccat atgcggcaaa
     1561 cagattttcg acctctcgag cgccctggcg gggcgggacg gcgggcagcc ggtccatttc
     1621 gactgcgcct tggccagcgt ctccgagggg gagcgcctcg agccgaccga gcgcatagcg
     1681 tatatcggtc gaggctcgtt cgccgtcgtc gagtatcggg acaagagtat gaccgccttc
     1741 accgtcaagc gccgcctcca gtgggaaaaa gaaggcgaga agtacgactg gcggagggcg
     1801 atccagcagc gcataggcct ataagggcgg gttcgcgcct ttcccgatct ggccaaagcg
     1861 cctgaaacgc gcatactggt agcggaggat gtatggccga ggcttcgtat accgtgctcg
     1921 tggtcgacga ttccggcgcc aacagggcct tcctttccag catactggag gaggatggct
     1981 accgcgtcgc gcaggctgcc gacggcgagg ccggcctggt cgccgccgcg acggagcggc
     2041 cggtcctgat cctgctcgac gtggtgatgc ctaggctcga tggcttcgag acgctcaagc
     2101 ggctcaaggc cgacccggcc accaggcgca tctccgttat catgctgacg agcctcggcg
     2161 accaggaatc caagctcaag gctttcgact acggcgccgt cgactatatc atcaagtccg
     2221 ccaacgaggc cgaagtccgg gccagggtgc gcgtccacgt gcagctagcg ctcgccaacg
     2281 aggagctgat cgaggccagg gccgccagcc tgcggcagat ttccgaggcc cagagatccc
     2341 tcctggtcaa gccgaccgag ctgcctgagg cccgcttctc ggtctactat cgctcccttc
     2401 acgaggctgg cggcgatttc tacgacgtcg tgccggtgtc cgacggcgtc tatttctacc
     2461 tgatagcgga
//
LOCUS       DAOPHV010000418         2466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_59904_length_2466_cov_3.276234, whole genome shotgun sequence.
ACCESSION   DAOPHV010000418 DAOPHV010000000
VERSION     DAOPHV010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59904_length_2466_cov_3.276234"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1541
                     /gene="murJ"
                     /locus_tag="PLQ29_10265"
     CDS             15..1541
                     /gene="murJ"
                     /locus_tag="PLQ29_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPG87072.1"
                     /translation="MVSRVLGLIRDRTRGALMGTGVLADAFTVAFSVPNLFRRLFAEG
                     SMSVAFIPTVKAYLSEGDREKTEEFLSSMFTALGLAVCAVVAAGVAASPLLVGLFDSE
                     PVETTILMRMMFPFLALVSIAAFFQGILNAYGSFTPSGFAPILFNLSFIFVPMLLGGL
                     AVNPARAMAVGVIVGGTFQALCQLPYVLRFGARFGLVSPRRAFSNPGVRRVLSLIAPT
                     ILGMAAYEVNGLVSTSLATRAGTGAATALSFSLRLQELVLGVFVVSVGTVLLPELSGL
                     AAEGDWKRYRERLTRALDAIALITVPVAVISILERIDIVSLLFRTQAFGDDSVAQTAS
                     AFFFHMLGLPFIGANRILAPAFYARGDTRTPAWSGILAVGVNVAAAFALQPAMGGAGI
                     ALALSVASAVNTAALIVMMVRMGVDGLGEALGAVAHYVVKLLAYSAIAATPVLLLRRP
                     LAEAFGGSSSGLVAYGAPFTLSALAFGAVGLGLLAVTKDRAAGFLASSVTGRSKRRSN
                     "
     gene            1606..1678
                     /locus_tag="PLQ29_10270"
     tRNA            1606..1678
                     /locus_tag="PLQ29_10270"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1639..1641,aa:Thr,seq:ggt)
     gene            1931..2104
                     /gene="rpmG"
                     /locus_tag="PLQ29_10275"
     CDS             1931..2104
                     /gene="rpmG"
                     /locus_tag="PLQ29_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPG87073.1"
                     /translation="MAGKKQVVEIIALACTECKRRNYTTTKNRKTKQEKLELMKYCKW
                     DHKHTLHKETKVK"
     gene            2186..2258
                     /locus_tag="PLQ29_10280"
     tRNA            2186..2258
                     /locus_tag="PLQ29_10280"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2219..2221,aa:Trp,seq:cca)
BASE COUNT          346 a          785 c          818 g          517 t
ORIGIN      
        1 tgtcggcgct gacgatggtc tcgcgggtgc tcggcttgat acgcgaccgt acgcgcgggg
       61 cgttgatggg cacgggcgtg ctggccgacg ccttcaccgt ggccttcagc gtgccgaacc
      121 tgttccggcg cctgttcgcc gagggctcga tgtccgtggc cttcatcccg accgtcaagg
      181 cctacctgtc ggagggcgac cgcgagaaga ccgaggaatt cctgtcgtcg atgttcaccg
      241 cgctcggcct cgcggtctgc gccgtcgtcg ccgccggcgt cgccgcgtcg cctctcctcg
      301 tcggcctgtt cgactcggag ccggtcgaga cgaccatcct gatgcgcatg atgttcccgt
      361 tcctggcgct cgtgtcgatc gcggccttct tccagggcat cctgaacgct tatggctcgt
      421 tcacgccgtc gggcttcgcc cccatcctgt tcaacctgag ctttatcttc gtaccgatgc
      481 tgttgggcgg cctcgcggtc aacccggcgc gcgccatggc ggtcggcgtc atcgtaggcg
      541 gcacgttcca ggctctgtgc caactaccgt acgtgctgcg cttcggcgcc cggttcggtc
      601 tcgtgtcgcc tcggcgcgcc ttctcgaacc cgggcgtgcg ccgcgtgctg tctctgatcg
      661 ccccgaccat cctgggcatg gccgcttacg aggtgaacgg gctcgtctcg acctcactgg
      721 cgacgagggc cggtaccggc gccgcgaccg cgctcagctt ctcgctacgc ctccaggagc
      781 tggtgcttgg cgtgttcgtc gtatcggtcg gcaccgtgct cctgcctgag ctgtcgggcc
      841 tggcggccga gggcgattgg aaacgctatc gggagcgcct gacgagggcg ctcgacgcca
      901 tcgccctgat caccgtgccg gtggccgtca tctccatcct cgagcggatc gacatcgtgt
      961 ccctgctgtt caggacccag gccttcggcg acgattcggt ggctcagacc gcgagcgcat
     1021 tcttcttcca tatgctcggc ctgccgttca tcggggcgaa ccgcatcctg gccccggcct
     1081 tctacgcccg gggcgacacg aggacgccgg cctggtccgg catcctcgct gtcggtgtga
     1141 acgtcgccgc ggcgttcgcg ctgcaaccgg ccatgggagg ggcgggcata gctttggccc
     1201 tgtccgtagc gagcgccgtg aatacggccg cgctgatcgt catgatggtc aggatgggcg
     1261 tggacggtct cggcgaggca ctcggggccg tggcgcacta tgtcgtgaag ctattggcct
     1321 attccgcgat cgccgcgacg ccggtcttgc tactgcggag gccgcttgcc gaggccttcg
     1381 gcggctcgag ctccggcttg gtggcctacg gtgcgccgtt cacgctgtcg gcgctcgcgt
     1441 tcggagcggt cggcctcggc ctgcttgccg tgacgaagga tagggccgct ggcttcctcg
     1501 cgtcctcggt gacgggtagg agcaaaaggc gttccaatta ggactgatcg tcgccgcgct
     1561 attgacaaag gcggcgacgg ttggtattgt accggcgctt ataacgccct tgtagctcag
     1621 ttggcagagc acatccatgg taaggatgag gtcatcggtt cgattccgat cgagggctct
     1681 ctgttcgcct ttggcgaacc aggcctgcaa gtcggtcgtc gctccgcgcc gtccgatcga
     1741 gggctctctg cgcatcgctg atgcgccagg cctgcaagtc ggttgacgct tcgcgtcatc
     1801 cgatcgaggg ctcttcgtag gtgtctgggg ctggtcggcg cgattgacgc ggcgcgtcgc
     1861 gacgtaccgg ggctccattt tttttcgacg atcgccgagg cggccgtcaa tcttccggga
     1921 gggtcggacc atggctggaa agaagcaggt cgttgagatc atcgcccttg catgcaccga
     1981 atgcaagcgc cgaaactata cgacgacgaa gaacaggaag accaagcagg agaagcttga
     2041 gctcatgaag tactgcaagt gggatcataa gcataccctg cacaaggaga cgaaggtaaa
     2101 gtaagccttc gtctggcgat gcggtctcgg ccgtatcgcg tcgtgcgcct gcccggctcc
     2161 cctcgagccc ggcaggcgga cgattaggcc agtagctcta atggtagagc gccggtctcc
     2221 aaaaccggat gttgagggtt cgagtccttc ctggcctgta catcgcacgg gcgtcgccgt
     2281 ttttgttttt tggcggcgga tccggacaga ccgcgttttc ggccgaagac gcggggtttc
     2341 tggggccttg tggtttctgg aacccatatc ccggcggccg tcggccgtcg cgctcgaacc
     2401 gcgtcctcta gtaggcgcgg tccgtccgtt tacggaggcg acatgaatag gatcatcggt
     2461 ttctac
//
LOCUS       DAOPHV010000419         2466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_59915_length_2466_cov_2.486520, whole genome shotgun sequence.
ACCESSION   DAOPHV010000419 DAOPHV010000000
VERSION     DAOPHV010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59915_length_2466_cov_2.486520"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..1198
                     /gene="ugpC"
                     /locus_tag="PLQ29_10285"
     CDS             80..1198
                     /gene="ugpC"
                     /locus_tag="PLQ29_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HPG87074.1"
                     /translation="MAKVELRNISKVYEGGVKAVENANIVVEDKEFVVFVGPSGCGKS
                     TTLRMVAGLEDISSGELYIDGDLVNDVPPKDRNIAMVFQNYALYPHMTVYDNMAFGLK
                     IRKLPKAEIDQRVREAAKILDIEKYLDRKPKQLSGGQRQRVAVGRAIVRNPKVFLFDE
                     PLSNLDAKLRVQMRAEIIDLHQRLQATMIYVTHDQVEAMTMGDKIVVMKDGLVQQIGS
                     PLYLYNHPINKFVAGFIGSPPMNFVTVKVLEEGGRLVLDEGSSRMTPSAEHAPSLKAY
                     VGKEVVLGLRPEDLPYDETGTGFKTTISVVEPLGAEIHLWAKTSSSQIVSKVPPHHTF
                     KIGETVCFAPVMEKARYFDPVSERSILPVAEVEGPSPN"
     gene            1285..>2466
                     /locus_tag="PLQ29_10290"
     CDS             1285..>2466
                     /locus_tag="PLQ29_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87075.1"
                     /translation="MMRSAVDSIIGLVDRIDSIERETEDIYLRLGTVFHAVKKAVDAS
                     AASAEQTIAAVLSQHRGGQSADVAKRRSEDFIDDATRYFKKTADIERTFLDSVETGIR
                     SLSALEEVIDRIRSDSEEMELVSLNAMTVALKSGAAGRAFSVITDELKRLSGRTIRHA
                     DNLSMAGSLLLERLDALKLTLDELSAAQSSFFEAAKRALEEGFITLDREVDGSARDLR
                     GLAESASRVRSPIADIMQEVQLQDIIRQSLDHVRLSLKAARPDAAPVRSATSVAAALA
                     TAAIGGHADDDISAGFFEDISPQEERAFLIEIARLSASLLDDVGSQVRASLERFHAGI
                     AGVNEVVSSVESSRSAIIAGRRAIDHDDLRDRTRAYMAAKERAVLEASEISESVRKLD
                     ER"
BASE COUNT          465 a          814 c          772 g          415 t
ORIGIN      
        1 gcgcgttgtt cgttgacggc caaggaaaag cggtttatcc ttcgttccat aagatacccc
       61 ccgaacggag gagcctcaaa tggcaaaggt cgagctcagg aacatctcga aggtgtacga
      121 gggcggcgtg aaggcggtcg agaacgccaa catcgtcgtc gaggacaagg aattcgtggt
      181 attcgtcgga ccctcgggtt gcggcaagtc cacgaccctc cgcatggtcg ccggtctcga
      241 ggacatcagc tccggcgagc tgtacatcga cggcgacctc gtcaacgacg tgccgcccaa
      301 ggaccgcaac atcgccatgg tgttccagaa ctacgccctg tacccgcaca tgacggtcta
      361 cgacaacatg gccttcggcc tcaagatccg caagctgccc aaggccgaga tcgaccagcg
      421 cgtgcgcgag gcggccaaga tcctggacat cgagaagtac ctcgaccgca agcccaagca
      481 gctgtccggc ggacagcgcc agcgcgtcgc ggtcggccgc gccatcgtgc gcaacccgaa
      541 ggtcttcctg ttcgacgagc ctttgtccaa cctggacgcc aagctgcgcg tgcagatgcg
      601 cgccgagatc atcgatctgc atcagcgctt gcaggcgacg atgatctacg tgacccacga
      661 ccaggtcgag gccatgacga tgggcgataa gatcgtcgtc atgaaggacg gcctcgtgca
      721 gcagatcggt tcgcccctgt acctgtacaa ccacccgatc aacaaattcg tcgccggctt
      781 cataggctcg ccgccgatga acttcgtcac cgtcaaggtg ctcgaggagg gcggcaggct
      841 cgtgctcgac gagggttcga gccggatgac gccgtcggcc gagcatgccc cgtcgctcaa
      901 ggcctacgtc ggcaaggagg tcgtgctcgg cctccgcccc gaggacttgc cctacgacga
      961 gaccggtacg ggcttcaaga cgacgatcag cgtcgtcgag ccgctcggcg ccgagatcca
     1021 cctgtgggcc aagaccagct ccagccagat cgtgtccaag gtgccgccgc accacacctt
     1081 caagatcggg gagaccgtct gtttcgctcc ggtcatggag aaggcccgtt acttcgatcc
     1141 tgtcagcgag cggtccatcc tgccggtggc cgaggtcgaa ggcccgtcgc cgaactgaac
     1201 cgcgtctcgg agccgctatc gacgggccgc cctccggggc ggcccgttct tgtaactggc
     1261 gaccggattc tctatagtag gtgcatgatg cgatccgcgg ttgattcgat aatcggcctg
     1321 gtagacagga tagactcgat agagcgcgag acggaggata tctacctccg cctgggtact
     1381 gtgttccatg ccgtcaagaa ggcggtggac gcgagcgccg cctccgccga gcagactatc
     1441 gcggccgtac tcagccagca tcgcggcggc cagagcgccg acgtggccaa acgccgctcc
     1501 gaggacttca tcgacgacgc tacccgttat ttcaagaaga ccgccgacat cgagcgaacc
     1561 tttctggata gcgtcgagac cggcattcgg agcctctccg ccctcgagga ggtcatcgat
     1621 cgcatacgct ccgactcgga ggagatggag ctggtgtcgc tcaatgcgat gaccgtggcg
     1681 ctcaagtccg gcgcggccgg ccgcgcgttc tcggtgatca ccgacgaact caagcggctt
     1741 tccggccgta ccatacgcca tgccgacaac ctgtcgatgg ccggctccct gcttctcgaa
     1801 cgcctggacg cgctcaaact gaccctggac gagctatcgg ccgcccagtc gtccttcttc
     1861 gaggccgcca agcgggcgct ggaggagggc ttcatcacgc tggaccgcga ggtggacggc
     1921 tcggcccggg atctccgagg actggcggag tcggcgtcgc gggtacgttc cccgatagcc
     1981 gacatcatgc aggaggtcca gctccaggac attatcaggc agtcgctcga tcacgttcgg
     2041 ttgtcgctca aggccgccag gcccgacgcc gcccccgtcc ggtccgccac ctcggtggcc
     2101 gcggcgctcg ccaccgcggc cataggcggt cacgcggacg atgacataag tgcgggcttc
     2161 ttcgaggata tcagccccca ggaggagcgc gccttcctga tcgagatagc gaggctatcg
     2221 gcctccctgc tcgacgatgt cggctcccag gtccgggcca gcctcgagcg cttccacgcc
     2281 ggaatcgctg gcgtcaacga ggtcgtctcc tcggtcgagt cgagccgctc ggctatcatc
     2341 gccggcaggc gtgcgataga tcacgacgac ttgcgtgata ggacgcgcgc ctacatggcc
     2401 gccaaggagc gcgccgtact cgaggcctcg gagatcagcg aatcggtacg caagctcgac
     2461 gagcgc
//
LOCUS       DAOPHV010000420         2465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_59930_length_2465_cov_3.274689, whole genome shotgun sequence.
ACCESSION   DAOPHV010000420 DAOPHV010000000
VERSION     DAOPHV010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59930_length_2465_cov_3.274689"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..1259
                     /locus_tag="PLQ29_10295"
     CDS             39..1259
                     /locus_tag="PLQ29_10295"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPG87076.1"
                     /translation="MEMQTVIADQYIARRRKPIRISVDRGFSHSLADGRIAMKPARRE
                     SRPVTLEPSEPSWRQSIGRRAKARPNQPGGRVAKRVAVIDSISGLVEALASPRVATPI
                     AGIAGAIALMVAIAAVGAFGRPADLTSFVLPDDGSSAAILLDALSPAADYGADSIPTP
                     ELPMTLRIATYTVAKNDTLDAISRRYGLRMDTLISVNGLGDARRIISGSTLKVPNLDG
                     VVHTVTKGESLAGIAAAKGVSVLDIVDANDLASQTILPGQSLFIPGARLSSYDLKKAL
                     GQLVIWPINGRISSNFGYRPNPFTGVRQFHNGLDIVGPLNATVKAAMDGKVAETGYSA
                     VFGNFVILSHPEGYQTLYAHLNKISVKAGVSIAQGSPVGLLGSTGYSTGPHLHFGVFK
                     RGAPLDPLKLLKGG"
     gene            1354..1497
                     /locus_tag="PLQ29_10300"
     CDS             1354..1497
                     /locus_tag="PLQ29_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87077.1"
                     /translation="MKKLVIGIIAVLAIVTFIRDFSVSPRQVVAQDVGQTMQSSMGTI
                     VTN"
     gene            1731..>2465
                     /locus_tag="PLQ29_10305"
     CDS             1731..>2465
                     /locus_tag="PLQ29_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPG87078.1"
                     /translation="MSARTNKSISEDTLKMYYERVKQIPLLSAEDERDLSMRIQAGDE
                     AARTQLIEANLRLVIKIARAFSNFDVPLIDLIQEGNMGLIKAAERFDYRRNVRFSTYA
                     SWWIKQSVTRSLVNSRRAIRLPHRKEELIRRIHRTYGYLSQALSREPSSLEIANELNI
                     DEKNVQEALEMSGAIMPLEGDSDDSESGCVLDVYADETYSPDAAFARDCLREKTVSLL
                     EALLEKERQVLIYRFELYGNKRKTLKG"
BASE COUNT          533 a          761 c          704 g          467 t
ORIGIN      
        1 cctcaacgcc gcgccataat gcgccgatag ataatcgtat ggaaatgcag actgtcatcg
       61 cggatcagta tatagcaaga aggcgaaagc cgatacgaat cagcgtcgat cgcgggttct
      121 cgcatagctt ggccgacggc cgcatcgcca tgaaaccggc caggcgcgag agccggcccg
      181 tgacgctcga gccgagcgag ccgtcatgga ggcaatccat cggcagacgc gccaaggctc
      241 ggcccaacca gccaggcggt cgggtcgcaa agcgggtagc cgtcatcgat tccatctccg
      301 ggctcgtcga agcgctcgct tctccgaggg tcgctacgcc gatcgccggg atcgccggcg
      361 cgatagcgct catggtcgct atcgcggcgg tcggagcctt cgggcgcccc gccgacctca
      421 cctccttcgt cctacctgac gacggctcgt ccgcggcgat cctactcgac gcgctatcgc
      481 cggccgccga ctatggggcc gattcgatac cgacccccga gttgccgatg acgctcagga
      541 tagcgaccta tacggtagcg aagaacgata cgctcgacgc catttccagg cgatacggcc
      601 tacggatgga cacgctgatc agcgtcaacg gcctcggcga cgcgcgacgc atcatctcag
      661 gctcgacgct caaggtgccg aacctagacg gcgtcgtcca tacggtgacg aagggcgagt
      721 cgctcgcagg catcgccgcg gccaagggcg tctccgtgct cgatatcgtc gacgcgaacg
      781 atttggccag ccagaccatc ctgccgggcc agagcctatt catccccggg gcgaggctgt
      841 cctcgtacga cctcaagaag gcgctcggcc agctcgtcat ctggccgatc aacggcagga
      901 tcagctcgaa cttcggctac cgccccaatc cgttcaccgg cgtcaggcag ttccataacg
      961 gcctggacat agtcggcccg ctcaacgcta cggtgaaagc cgcgatggat ggcaaggtgg
     1021 cggaaaccgg ctacagcgcc gtcttcggca atttcgtcat cctgagccat cccgagggat
     1081 accagacgct ctacgcccac ctgaacaaga tcagtgtcaa ggccggcgta tccatagctc
     1141 aaggctcgcc ggtcggttta ctcggttcta ccgggtatag taccggcccc catctgcact
     1201 tcggcgtctt caagcgaggc gcgcctttgg acccgctcaa gctgctcaaa ggcggctgat
     1261 tatggcagga atccggcact tcggctccag gcgcctccgg acgcttgcga ttcatacgac
     1321 gagcgagtac gatcggcgag gtacgggggg tatatgaaga agctcgtcat cggaatcata
     1381 gccgtattgg ccatcgtcac cttcatacgc gatttctccg tttcgccgcg tcaagttgtc
     1441 gcccaagacg tcgggcagac catgcagtcg agtatgggta ccattgtcac caactgatgg
     1501 ctacgcgctc gagactccta gaccgcccct tcacggggcg gtttattatt ttctagacgc
     1561 ataaaaagcg tgtttgtgta ctgaagtaga ctattgtttc tgccgacatg ctatcgcaag
     1621 ttattattgt aagcttattt ggttgtttca taattactta gtgacgagag gcccatgctc
     1681 tcaaatttct ggcacgcttc ttgcatcaaa tatggcagag gtaacaggaa atgagtgcca
     1741 gaacaaataa gtcaatatct gaggacacgc ttaaaatgta ctacgagcgg gtaaagcaga
     1801 taccgctcct cagcgccgag gacgagcggg atctctcgat gcgcatccaa gccggcgacg
     1861 aggccgccag gacgcaactc atcgaagcca atctcaggct ggtcatcaag atagcgcgtg
     1921 ccttctcgaa tttcgacgtt ccccttatcg atcttatcca ggaagggaac atgggcctga
     1981 tcaaggcggc cgagcggttc gactaccgca ggaacgtacg tttctccacc tatgcctcgt
     2041 ggtggatcaa gcagtcggtg acccgctcgc tcgtcaactc acggcgcgcc attcggcttc
     2101 cccaccgtaa ggaagagctg atacgacgca tccacaggac ctacggatac ctgtcccagg
     2161 ccctctcacg ggaaccgagc agcctcgaga tagcgaacga gctcaacatc gacgagaaga
     2221 acgtgcagga agccttggag atgtcgggcg ccatcatgcc gctcgagggc gactccgacg
     2281 attcggagag cggctgcgta ctggacgtct acgctgacga gacctacagc cccgacgcgg
     2341 ccttcgcccg cgattgcctt agggaaaaaa ccgtcagcct gctcgaggct ctgctcgaga
     2401 aagaacgcca ggtattgatc tatcgcttcg agctgtacgg taacaagcgc aagacgctca
     2461 agggc
//
LOCUS       DAOPHV010000421         2461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_60078_length_2461_cov_3.136741, whole genome shotgun sequence.
ACCESSION   DAOPHV010000421 DAOPHV010000000
VERSION     DAOPHV010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60078_length_2461_cov_3.136741"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLQ29_10310"
     CDS             complement(<1..265)
                     /locus_tag="PLQ29_10310"
                     /inference="COORDINATES: protein motif:HMM:NF016352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87079.1"
                     /translation="MREAPLADFIGEGEIPWHRIWFIKAGPLIVWDRRERIDLVFGSG
                     ASPAPDLAAIQRACTPLVPEAPPPAARRRSSGAAEFTPIPCLRF"
     gene            399..2177
                     /gene="mutL"
                     /locus_tag="PLQ29_10315"
     CDS             399..2177
                     /gene="mutL"
                     /locus_tag="PLQ29_10315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPG87080.1"
                     /translation="MNASRIRVLAPEVAKRIAAGEVIDRPAAALRELLDNAIDSGAGS
                     VVVELEGGGIDSLRVVDDGSGMGKDDLALSVLPHATSKIRELDDLLSLSTLGFRGEAL
                     SSMAAVAGLEIVSATDDDEAWRLVSEPGRPATVTPSRGSRGTAVTLRDLFASFPARRQ
                     FMKRPAAEAGACRAVFVDKAMAFPDVAFRLSSGGRPLLSLRPSSLAERVIAAQAPDQA
                     PELFRELVASGDGFSARIVAGLPSVYRTDRRHIQVFVNGRRVQEYGLSQAIEYAYRGA
                     LPGGAWPFAYAFVEVDPRLADFNIHPAKKEVRLRNLDEIRSGIIRAIRDYLGAQSRLA
                     SPFMPPPSLSGRPPAVEASSAEGLFSSAAFVPEARERPYPADRSSWSSIADAAAKARE
                     TPRRAAGDVTPVGGRDDGLENGFRYIGRALGVFLAFERGDELVLLDQHAAHERYLYNE
                     MMAGRAVSQELLVPVVYEPESEAEDRYIAAATETLAQAGYRLSKEGGAWLLEAAPAML
                     PEAMTGSVFALLRSRPDPAELLRETVAQSACKAAVKDGDELDETGAIALIEAALSMPE
                     PRCPHGRPIWYRLSRDDLFRAVRRLV"
     gene            complement(2203..>2461)
                     /locus_tag="PLQ29_10320"
     CDS             complement(2203..>2461)
                     /locus_tag="PLQ29_10320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG87081.1"
                     /translation="APRDATIRLNLARAYVQSGALPKARDAYIELLKLAPATWEAMYE
                     LGKAYLSLGDQASAKKTLSELLAKKPDFRARVEVETILSGL"
BASE COUNT          382 a          843 c          852 g          384 t
ORIGIN      
        1 cgaagcggag gcaggggatc ggcgtgaact cggcggcgcc ggagctgcgg cggcgggccg
       61 cgggcggcgg ggcctcgggg acgagcgggg tgcaggcgcg ctggatcgcg gcgaggtcgg
      121 gggccggcga ggcgccggag ccgaacacga ggtcgatgcg ctcgcggcgg tcccacacga
      181 tcagcgggcc ggccttgatg aaccagatgc ggtgccaggg gatctcgccc tcgccgatga
      241 agtcggcgag cggggcctcg cgcacgcccg cgaagcgctc ctcgtagccg aggctgcact
      301 tctagcctcc ggcatccgcg tagcttgggc cgcccgcgtg ggcggccctt tgtatcgcgc
      361 gggtgacagg tacggccgcc agggtgtata cagacagcat gaacgcctca aggatacggg
      421 tactagcgcc cgaggttgcc aagcgcatcg cggccggcga ggtgatagac cgaccggccg
      481 ccgcgctccg cgaactcctg gacaacgcca tcgactccgg cgccgggagc gtggtcgtcg
      541 agctggaggg cggaggcatc gacagcctcc gggtcgttga cgacggctct ggcatgggca
      601 aggacgacct ggctctctcg gtcctgcctc acgcgacctc gaagatacgc gagctcgacg
      661 acctcctgtc gctgtcgacc ctcggcttca ggggcgaggc cctgtcgtcc atggcggcag
      721 tcgccggctt ggagatcgtc agcgcgactg acgacgacga ggcgtggcgc ctggtatccg
      781 aaccagggag gccggcgacg gtaaccccct cgcgcggctc gaggggcacc gccgtgaccc
      841 tgcgcgacct cttcgcctcg ttccccgccc gcagacagtt catgaagcgc ccagccgccg
      901 aggccggagc ctgcagggcc gtcttcgtcg acaaggcgat ggccttcccg gacgtggcct
      961 tcaggctatc ctccggcgga cggccgctcc tttcgctccg gccgtcgagc ctggccgagc
     1021 gcgtcatcgc ggcccaggcc cccgaccagg cgccggagct gttcagggag ctcgtcgcct
     1081 cgggagatgg cttctcggcg aggatcgtgg cgggcctgcc gtcggtctac cgtaccgatc
     1141 ggcgccatat ccaggttttc gtcaacggcc gtcgcgtcca ggagtacggc ctctcccagg
     1201 ccatcgagta cgcgtatcga ggcgctctgc ccggcggcgc ctggcccttc gcctacgcct
     1261 tcgtcgaggt cgacccgagg ctagcagatt tcaatatcca tccggccaaa aaggaagtca
     1321 ggctcaggaa cctcgacgag atccgctccg gcatcatacg cgccattcgc gattacctgg
     1381 gtgcccagtc gaggctagcc tcgccattca tgcctccgcc ttcgctatca ggccggccgc
     1441 ctgccgtcga ggcgagtagc gccgaaggtt tgttcagttc cgccgccttc gtcccggaag
     1501 cccgcgaacg gccctaccct gccgaccgtt caagctggtc ctcgatcgcg gacgcagctg
     1561 ccaaggccag ggagacgccg cgccgcgcgg ctggcgacgt tacgcccgtc ggaggccgcg
     1621 acgatgggct cgagaacggc ttcaggtaca taggcagggc gctcggcgtc ttcctcgcct
     1681 tcgaacgggg cgacgaactc gtgctactcg accagcacgc ggcccacgag cgctacttgt
     1741 ataacgagat gatggccggc cgcgccgtgt cccaggaact gctcgtccca gtcgtctacg
     1801 aaccggagtc ggaggccgaa gaccgctata tagccgcggc gaccgagacg ctcgcccagg
     1861 ctggctaccg gttatcgaag gaaggcggag cctggctact cgaggcagcc ccggcgatgt
     1921 tgcccgaggc gatgaccggg tccgtgttcg cgttgctacg ctcacggccg gacccggccg
     1981 agcttctgcg cgagacggtg gcccagtcag cgtgcaaggc agccgtcaag gacggcgacg
     2041 agctcgacga gactggcgcg atcgccctca tcgaagcggc gctctccatg cccgagccgc
     2101 gttgccccca cggcaggccc atctggtata gattgtcgcg ggacgacctg ttcagggcag
     2161 tccgacgcct ggtctaaaat aggctcgccg aggggcggcg tctcagagcc cggacagaat
     2221 cgtctccacc tcgaccctgg ccctgaagtc gggcttcttg gcgagcagct cggacagggt
     2281 cttcttggcc gaggcttggt cgccgagcga caggtaggcc ttgcccagtt cgtacatggc
     2341 ctcccaggta gcgggggcca gcttcaatag ctcgatatag gcgtcccgcg ccttgggtag
     2401 cgcgcccgac tgcacatagg cgcgggccag attcagacgt atcgtcgcgt cgcgcggggc
     2461 c
//
LOCUS       DAOPHV010000422         2451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_60400_length_2451_cov_4.182805, whole genome shotgun sequence.
ACCESSION   DAOPHV010000422 DAOPHV010000000
VERSION     DAOPHV010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60400_length_2451_cov_4.182805"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="PLQ29_10325"
     CDS             complement(<1..1323)
                     /locus_tag="PLQ29_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPG87082.1"
                     /translation="MRRSNRVIARVFSLLVAAILPAILVFWVVISGLFVEAAATNANR
                     TKAALMNQADRLAFDLDLELTLLDSCLATAMARLTPSDGAASWEEPIARGVEAYKAAS
                     RWPLLVASVVLVEEGEAFGDPSADDAATHMIRLAPGGSFESGGRALVAILDQGVLAGT
                     VIPILAESYFGADTGFGDYAITLRAPSGERRFSSGAADPLEADLERPLFRDSSGFDVS
                     GFYEAFFMGGESWPMSYRTLSGFDRLPYPLGPPAPRMGSPRNGRSDDQFPIDRYRFQE
                     GGERGRWTLSVYGKRRSLALEAKLQAIGWSIASAAFLALLYGCVLMLYYAVRKSIRLT
                     ERERDFVASVTHELKTPVTVILSAGENLAEGIVEQRRVAEYGAIVAKEARRLAETVER
                     LLVMAGLQSTESVARSEPVEAADLIEAAIAKLRATASALGVSFSVYIAG"
     gene            complement(1345..>2451)
                     /locus_tag="PLQ29_10330"
     CDS             complement(1345..>2451)
                     /locus_tag="PLQ29_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPG87083.1"
                     /translation="DLDISALGLGCMRLPTRPDDQAAIDEDAADALLSDALEAGVNYL
                     DTAWPYHKERSEPWLGRAVERLGCRDVVLIATKAPTWLIEEPGDWERFLAEQLLRLRV
                     DRIDFYLAHALNRKRWRTMLETGGLEFLAKAKADGRIGHVGFSFHDDLDSFKEIVDGW
                     PDWEFCQVQYNYIDEEYQAGDAGIRYAAERGLGVIVMEPLRGGVLADVPDEVRKAFAS
                     YPIPRMAAEWALRHVLDRQEVVTALSGMGASSQLWENAAVASSARANSITTPERAAID
                     AARDWFRSRMATPCTACGYCRPCPAGVSIQGVFELWNDASMFGDREGARRRYATELAG
                     HGADACVACGECVPKCPQGIDIPAMLRRAAEALG"
BASE COUNT          401 a          831 c          832 g          387 t
ORIGIN      
        1 tcccgctata tacaccgaga atgagacccc gagggccgag gctgtcgccc gtagcttggc
       61 tatggcggct tcgataaggt cggccgcctc gacgggctcg ctcctggcta cggattcggt
      121 cgactgaagg cccgccatga ccagaagccg ttcgacggtc tcggccagcc gcctagcctc
      181 cttggcgacg atggcgccgt actccgcgac gcgccgttgc tcgacgatgc cctccgcgag
      241 attctcgccg gcggacagga tcacggtgac gggcgtcttg agctcatgcg tgacgctggc
      301 cacgaaatcg cgttccctct cggtcagccg tatcgacttc ctgacggcgt agtagagcat
      361 gagcacgcag ccgtagagca gggcgaggaa ggctgccgag gctatcgacc agccgatggc
      421 ctggagcttg gcttccagag cgagcgaacg tcgcttgccg tagaccgaca gggtccagcg
      481 gcctcgctcg ccgccctcct ggaacctata ccggtcgatc ggaaattgat catccgatcg
      541 gccgtttcgc ggcgatccca tcctgggcgc cggcggcccg aggggatagg ggagccggtc
      601 gaatccgctc agggtccggt aactcatcgg ccagctctcg ccgcccatga agaacgcctc
      661 gtagaagccg gagacgtcga agcccgatga atccctgaag aggggacgct cgaggtcggc
      721 ctcgagaggg tcggccgcgc cgctcgagaa acgccgctcc ccggaggggg cgcgtagcgt
      781 gatagcgtag tcgccaaacc cggtgtccgc tccgaaatac gattcggcca ggatggggat
      841 gacggtaccg gcgagcacgc cctggtcgag gatggccacg agcgcgcgtc ctccgctctc
      901 gaaggagccc ccgggcgcta ggcggatcat gtgggtcgcc gcgtcatcgg cgctcggatc
      961 gccgaaggct tcgccttctt cgaccaggac gacggaggcc acgaggagcg gccaacgcga
     1021 ggcggcctta tacgcctcga cgccgcgcgc gatcggctct tcccaggagg cggcgccgtc
     1081 cgacggcgtg agccgggcca tggctgtggc gaggcacgag tcgagcaggg tcagctcaag
     1141 gtcgaggtcg aaggccaggc ggtcggcctg gttcatcagc gccgccttcg tcctgttcgc
     1201 gttcgtagcg gccgcttcca cgaacaggcc gcttatgaca acccagaaga cgagtatcgc
     1261 gggtagtatc gccgctacca ggagcgagaa tacgcgcgcg atgacccgat tgctcctcct
     1321 catccggcct cctccgggct acgatcagcc gagggcctcg gccgcccgcc tgagcatggc
     1381 cgggatgtct atgccctgag ggcacttcgg gacgcactcg ccgcacgcga cgcaggcgtc
     1441 ggcgccgtgc cccgctagtt cggtcgcgta ccgcctgcgg gcgccctcgc ggtcgccgaa
     1501 catcgaggca tcgttccaga gctcgaacac gccctggatc gacacgccgg cggggcaggg
     1561 gcggcagtac ccgcaggcgg tgcacggcgt agccatgcgc gagcggaacc agtcccgcgc
     1621 ggcgtcgata gccgcgcgct cgggcgtcgt gatcgagttg gcgcgcgccg aggaggctac
     1681 cgccgcgttc tcccagagct gcgacgaggc gcccatgccg gagagcgccg tcacgacctc
     1741 ctgccggtcc agcacgtggc ggagcgccca ttcggcggcc atcctcggga taggatagga
     1801 cgcgaaggcc ttgcgcacct cgtccggcac gtcggccagc acaccgccgc gtagcggttc
     1861 catcacaatg acgcctaagc cccgctcggc ggcgtagcgg atgccagcgt cgcccgcttg
     1921 gtattcctcg tcgatgtaat tgtactggac ctggcagaat tcccaatcgg gccagccatc
     1981 cacgatttcc ttgaacgaat cgaggtcgtc gtggaacgag aagccgacgt ggcctatcct
     2041 tccgtcggcc ttggccttgg ccaggaactc cagccccccg gtctcgagca tcgtccgcca
     2101 gcgcttgcgg ttgagcgcgt gcgccaggta gaagtcgatc cggtcaacgc gcagccgcaa
     2161 gagctgttcc gcgaggaaac gttcccagtc gccgggctcc tcgatcagcc aggtcggggc
     2221 cttcgtcgcg atgagtacca cgtcgcggca gccgaggcgc tcgacggccc ggccgagcca
     2281 gggctcgctc cgttccttat gatagggcca ggccgtatcg agatagttga cgccggcctc
     2341 gagcgcgtcg gacaacaggg cgtccgccgc gtcctcgtcg atggccgcct ggtcgtcagg
     2401 gcgggtcggt aggcgcatgc agccgaggcc gagcgccgat atgtccaggt c
//
LOCUS       DAOPHV010000423         2451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_60409_length_2451_cov_3.533806, whole genome shotgun sequence.
ACCESSION   DAOPHV010000423 DAOPHV010000000
VERSION     DAOPHV010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60409_length_2451_cov_3.533806"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..630)
                     /locus_tag="PLQ29_10335"
     CDS             complement(184..630)
                     /locus_tag="PLQ29_10335"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HPG87084.1"
                     /translation="MRYRSAAVALACAASLLTRFGPARALRIRDEAGRPLATIAVPAT
                     FTLSFMHSVNLSRVDEEFSVGGGLTLERVLFDQLSTGMPSGDEDGFCVVDGRFETRPG
                     RRFDEIAVRVSPIPGHELRVDGRVRPLTYWAPTGNMVRFSASRESD"
     gene            complement(678..1628)
                     /locus_tag="PLQ29_10340"
     CDS             complement(678..1628)
                     /locus_tag="PLQ29_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HPG87085.1"
                     /translation="MKKISILLIAIALVAALSGCGAPKQSFVSIASGGTGGTYYPLAG
                     AMATIWNEGVKGMNATAQATGASVANVNLLREGKADVIMVQNDIAYYAVNGLELFKDK
                     AFPELKGMAILYPETCQLITLANSGINSVAGLKGKKVAVGAAGSGVEANARQIMEAAG
                     VTYADIQVQYLSFNEAASNLKDGNIDAAFLTAGHPTAAVQDISATKDIRVVKIDDAVV
                     AKLQSTYPFYTKTVIPANTYKGVDADTQTVAVQAMLAVSASLDAATVEKMLQTMFSAA
                     GTERLTAAHAKAGAMIKLENAQAGMSLPLHPGAEKFYGKK"
     gene            complement(1780..>2451)
                     /locus_tag="PLQ29_10345"
     CDS             complement(1780..>2451)
                     /locus_tag="PLQ29_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87086.1"
                     /translation="DLLSQEAVREEKVRLLRAVPQLSPAEAATLAVRGQYSGYRDEPS
                     VRPDSSTETFVAMRLEVDNWRWAGVPFILKTGKALSGRRTEIVAHFRPGETEVPGPAL
                     DANHLVLRIQPDEGVVLRFNAKAPDSAQAEREELVGMLRDSAARASGPYDRLLADAMA
                     GDPTLFIRFDETEEAWRLVDPVLAHWAERGADGLLQYADGGSGPDTARLLGVSMTRRG
                     RLAAG"
BASE COUNT          372 a          763 c          864 g          452 t
ORIGIN      
        1 tgtgtacagc tgaaaacagg tgaaggtgat ggctatggcc gagatcacct tggccatgag
       61 gcccgagtag acgcggaaat tcgattcctt gtcatacttc ttgaggatcg cttggctatc
      121 ggcctctgcc gtcgcgatcg catcgttcgt cgtcgattca ctcatcgatc gatctcctta
      181 aggttaatcg gattcgcgcg aagcgctgaa tcgtaccatg ttccccgtgg gcgcccagta
      241 ggtcagtggc cgcacgcggc cgtcgacccg tagctcgtgg ccggggatcg gcgagacccg
      301 gacggctatc tcgtcaaagc gcctgcctgg cctcgtctcg aagcgcccgt ccacgacgca
      361 gaacccgtct tcgtcacccg atggcatgcc ggtcgacagc tggtcgaaca gcacgcgctc
      421 gagcgtcagg ccgccgccca cggagaactc ctcgtccacg cgggacaggt tgaccgagtg
      481 catgaaggat agggtgaagg tggctgggac tgctatcgtc gcgagcgggc gccccgcctc
      541 gtcgcggatc cgcagtgccc gagcggggcc gaacctggtc agtagcgagg ccgcgcacgc
      601 gagcgcgacg gccgcgctac ggtatctcat acgagtcgcc ggcgccccat cgatgggccg
      661 ccggctcctt cctctcgtta cttcttgccg tagaacttct cggcgcccgg atgcagcggc
      721 aacgacatgc cggcctgggc gttctcgagc ttgatcatcg cgccggcctt ggcgtgggcg
      781 gcggtcaggc gctcggtacc ggcggccgag aacatcgtct ggagcatctt ctcgacggtg
      841 gcggcgtcga gcgaggcgga gacggcgagc atggcctgga cggccacggt ctgcgtgtcg
      901 gcgtctacgc ccttataggt gttcgccggg atgaccgtct tcgtgtagaa ggggtaggtc
      961 gactggagct tcgcgacgac ggcatcgtcg atcttgacga ccctgatgtc cttggtcgcg
     1021 gagatgtcct ggacggcggc ggtcgggtga ccggcggtca ggaaggccgc gtcgatgttg
     1081 ccgtccttca ggttgctggc ggcctcgttg aacgacaggt actgaacctg gatgtcggcg
     1141 taggtgacgc cggcggcttc catgatctgg cgggcgttgg cctcgacgcc tgaaccggcg
     1201 gcgcccacgg ctaccttctt acccttgagc ccggctacgg agttgatgcc ggagttggcg
     1261 agcgtgatca gctggcaggt ttcgggatag aggatggcca tgcccttcag ctcggggaag
     1321 gccttgtcct tgaacagctc gaggccgttg acggcgtagt aggcgatgtc gttctgcacc
     1381 atgatcacgt cggccttgcc ctcgcggagc aggttgacgt tggccaccga ggcgccggtc
     1441 gcctgggcgg tggcgttcat gcccttgacg ccctcgttcc agatggtcgc catggcgccc
     1501 gcgagcgggt agtaggtgcc gccggtgccg ccggaagcga tggatacgaa tgactgcttg
     1561 ggggctccgc agcccgagag ggcggcgacg agcgcgatcg cgatcaggag tatgctgatc
     1621 ttcttcattg tagttcctcc ttgttgagtg ccggtggata ggaatgcggt atggtactgt
     1681 tcctatgcac gcggcaaggg aacagtgtag tcgtttgccg ccgggaacgc aagtattttt
     1741 tattatcacg cctcgctcgg tcggcgaagc aacgccccgt cagccggcgg ccagccgacc
     1801 gcgcctcgtc atggagacgc cgagtagccg ggccgtgtcg gggccggagc ctccgtccgc
     1861 gtactggagc aggccatcgg cgccgcgctc cgcccagtgg gccagtactg ggtcgacgag
     1921 gcgccaggct tcctcggtct cgtcgaagcg gatgaacagc gtgggatcgc cggccatggc
     1981 gtcggccagc aggcggtcgt aggggcctga ggccctggcc gccgagtccc tgagcatgcc
     2041 gacgagctcc tcgcgctccg cctgggccga gtcgggggcc ttggcgttga agcgcagcac
     2101 cacgccctcg tcgggctgga tgcggagtac caggtggttg gcgtcgagcg cggggccggg
     2161 gacctcggtc tcgcccggcc tgaagtgagc cacgatctcg gtccggcggc cggatagggc
     2221 cttgcccgtc ttcagaatga aggggacgcc ggcccaacgc cagttatcga cctcgaggcg
     2281 cattgccacg aaggtctccg tggaggagtc gggccggacc gacggctcgt cgcggtagcc
     2341 ggagtactgg ccccgcacgg cgagcgtcgc ggcctcggcc ggagacagct gaggcacggc
     2401 ccgtagcagc ctgaccttct cctcgcggac cgcctcctgg gacaggaggt c
//
LOCUS       DAOPHV010000424         2438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_60855_length_2438_cov_3.874948, whole genome shotgun sequence.
ACCESSION   DAOPHV010000424 DAOPHV010000000
VERSION     DAOPHV010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60855_length_2438_cov_3.874948"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="PLQ29_10350"
     CDS             <1..1543
                     /locus_tag="PLQ29_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HPG87087.1"
                     /translation="AVLVNNELQSLDAHVLTNCSMEPITIDSAQGAFSYRRSLCFLAA
                     IAARDLFPDRDLVVGQAIGYGFYHRFQDGSVSGADIAALEKRMRELVAEDIPIAMQWW
                     SYEEALAYFRDHGQEDTLLLLELTNEPRIALNECSGFRDLHVTPLVPSTGVLRTFELR
                     PYDDGFLLRYPRKEKPDEIPAFEDDPLLYKITDDSRRRSGVLGVDSVGSLNRLAAPKR
                     IKDYITVAESLMTKTVAEIADQVAARRETVKVVLIAGPSSSGKTTTSKKLSIQLRVLG
                     FEPMVIGLDDYFVDRTRTPRDENGEFDFECLEALDVEYLNEQLLQLFAGEEVELPAFD
                     FKTGERRASGKRLRLGERTILIMEGIHGLNDRLTPRIPREQKFKIYVSALTQINLDEH
                     NRISTTDNRLIRRMVRDNQFRGRSARDTIRMWPSVQRGERLHIFPFQNGADAALNSAL
                     DYELGVLKVYAEPLLRSVKPMDPEYCEAKRLLNFLAFFAPIPAQYVPQDSLVREFIGE
                     SSFKY"
     gene            1774..>2438
                     /locus_tag="PLQ29_10355"
     CDS             1774..>2438
                     /locus_tag="PLQ29_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPG87088.1"
                     /translation="MKKVLTALAIALCVVSVTTAQVKNGPIVDKVIYTVSMDQSIAMK
                     DIVEGKADVFFQAVPANILRTLSEAEKAKLDIYAVPSGSWSLMLNPIPNKAPYTWTKS
                     DGVTEFNPFAIREVRYALNWLINRKKLVDEILLGDGEPMFTPMTPGQPGTYRYNLIAS
                     QLGMTPTGNERKAIADIDAAMTAASQLPENKGKLVKKGQFWQYNGQDVTIKFLIRVDD
                     PTGR"
BASE COUNT          479 a          827 c          720 g          412 t
ORIGIN      
        1 cgccgtcctc gtcaacaacg aactccagtc cctggacgcc cacgtgctga ccaactgctc
       61 catggaaccg atcacgatcg actcggccca gggcgccttc tcgtaccgcc gctcgctctg
      121 cttcctcgcc gccatcgccg cgcgagacct ctttcccgac cgtgacctgg tcgtcggcca
      181 ggccatcggc tacggcttct accaccgctt ccaggacggt tcggtctccg gggccgacat
      241 cgccgccctc gagaagcgca tgcgggagct cgtggccgag gacatcccca tcgccatgca
      301 gtggtggagc tacgaggagg cgctcgccta cttcagggac cacggccagg aggacacgct
      361 gctcctcctc gagctgacca acgagccgag gatagcgctc aacgagtgct caggcttccg
      421 cgacctgcac gtgacgccgc tcgtaccatc gacgggcgtg ctcaggacct tcgagctccg
      481 gccctacgac gatggcttcc tcctgcgcta tccgcgcaag gagaagcccg acgagatacc
      541 ggccttcgag gacgaccccc tcctctataa gatcaccgac gactcgcgcc gccgctcggg
      601 cgtgctcggc gtcgacagcg tcggctcgct caacaggctc gccgcgccca agcgcatcaa
      661 ggactacatc acggtcgccg agtcgctgat gaccaagacc gtcgccgaga tagccgacca
      721 ggtggccgcc aggcgagaga cggtcaaggt ggtgctgata gcggggccgt cgagctccgg
      781 caagacgacg acgtcgaaga agctgtcgat ccagctgcgg gtgctcggct tcgagcctat
      841 ggtcataggc ctggacgact atttcgtcga ccggacgcgc actccgcgcg acgagaacgg
      901 cgagttcgac ttcgagtgcc tcgaggccct cgacgtcgag tacctgaacg agcagctcct
      961 ccagctgttc gccggcgagg aggtggagtt gccggccttc gacttcaaga ccggcgagcg
     1021 cagggcctcg ggtaagcgcc tcaggctcgg ggagcggacc atcctgatca tggagggcat
     1081 ccacggcttg aacgacaggc tgacgccgcg catcccccgc gagcagaagt tcaagatcta
     1141 cgtgtcggcc ctcacgcaga taaacctgga cgagcacaac cgtatctcga ccacggacaa
     1201 ccggctgata cgccgcatgg ttcgcgacaa tcagttccgc ggccgctcgg cccgcgacac
     1261 gatcaggatg tggccgagcg tgcagcgcgg tgagcgcctc catatcttcc cgttccagaa
     1321 cggggcggac gcggcgctga actcggctct cgactacgag ctcggcgtgc tcaaggtcta
     1381 tgcggagccg ctcctgcgct cggtcaagcc gatggacccg gagtactgcg aggccaagcg
     1441 cctcttgaac ttcctggcct tcttcgcgcc cataccggcg cagtacgtgc cccaggatag
     1501 cctggtgcgc gagttcatcg gggagagttc cttcaagtat tgacgccggg acgcggggcc
     1561 gcctgggcga tagtcgacgc gccgcccgga cggcctcggg cggcgttcag cgcgcttctg
     1621 catatagcct cgtaactatc tataatatcg aaagttaagt ttagactagc cattgccaca
     1681 attttacgca tccatgcaac tcgattcttt acaaatcggc caagatgcta cacaatgccc
     1741 tatcttttga gttctcatgt aaggaggttt tacatgaaga aggtactaac cgcgctggca
     1801 atagccttgt gcgtcgtatc cgtgacgacc gcccaggtca agaacggccc gatcgtcgac
     1861 aaggtgatct ataccgtcag catggatcag tccatcgcca tgaaggacat cgtggaaggc
     1921 aaggccgacg tcttcttcca ggccgtcccc gcgaacatcc tcaggacgct cagcgaggcc
     1981 gagaaggcca agctggacat ctacgccgtt ccgtccggat cgtggtcgct catgctcaac
     2041 ccgatcccga acaaggcccc gtatacctgg accaagagcg acggcgtgac ggagttcaac
     2101 ccgttcgcca tccgcgaggt ccgctacgcg ctgaactggc tcatcaaccg caagaagctg
     2161 gtcgacgaga tcctgctcgg cgacggcgag ccgatgttca ccccgatgac gcccggccag
     2221 cccggcacgt atcgctataa cctgatcgcc tcccagctcg gcatgacccc caccggcaac
     2281 gagcgcaagg ccatcgccga catcgacgcc gcgatgaccg ccgcctcgca gctgcccgag
     2341 aacaagggca agctggtcaa gaagggccag ttctggcagt acaacggcca ggacgtcacg
     2401 atcaagttcc tgatccgcgt cgacgacccg acgggccg
//
LOCUS       DAOPHV010000425         2437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_60902_length_2437_cov_3.641478, whole genome shotgun sequence.
ACCESSION   DAOPHV010000425 DAOPHV010000000
VERSION     DAOPHV010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60902_length_2437_cov_3.641478"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2437)
                     /locus_tag="PLQ29_10360"
     CDS             complement(<1..>2437)
                     /locus_tag="PLQ29_10360"
                     /inference="COORDINATES: protein motif:HMM:NF020083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPG87089.1"
                     /translation="EFQRVALSATVEPFDVAASFAAGSRLVRGPDGAARYEPRAMAVV
                     APPASKRYELSVAWPAAPLRVRPPTAGPDERETDDRMIDEPAGRYDAIADELAERLER
                     ERSTIVFADSRRRAERLAAMLNERSGDGTAWAHHGSLSKDVRKAVEARLKDGRLRCVV
                     ATSTLELGIDVGAIDLVALAGSPQRADQLLQRAGRSGHRVGLTSRAVVYPFHGLDLLA
                     AAAAVGAALDRDIDPMRPPREPLDLLAQVVLSMVLFGERRLGELYDEVRSFPPYADLA
                     RADFDAVVGMLAGRFAGSRVRELAGRAYLDEEHGTLRAKEGAAMLLYSAGGSIPDRGY
                     YAMRLADGTRLGELDEEFVFERRVGNSFSFGSQAWRIVDIGDEAVTVAPLDRDADFMP
                     FWKAEKAARGASIARRMLGLCAAFDADPDALAAELRGRLGFSPEAAKALVAFLSSQSR
                     AQRGERLPSPGSVVIEAFRDPEAKAGATALIVHTLRGSAVNEPLGLALSSLLRDRLGV
                     PVERLSNDDSVSLSMPLVDRDEAVRALVDALRSLADPGTLSRALRGALVGSAIFGAAF
                     RENAGRALLLPRAGFGRRTPLWVTRLRAKRLLERTSGYDGFPIAAETWKTVLEQRFDL
                     ASLSALCAGLADGSVAAPIFSPAAPSPFARQCGWAETNRYLYEDDESTISSSGTPAGG
                     AAGAAEAAITRAMSDPAARPRVDPAIASAYGRRLRRELPGWAPSTPDGLADWVEERLV
                     IPLDGAYRAAWMSGFSCGSSSRSSSGDKIFSPCTPLRKPSSRSCCSFTVSSSVNATFS
                     EPFFWNGTCSSAQR"
BASE COUNT          361 a          807 c          940 g          329 t
ORIGIN      
        1 tctctgcgca gagctgcatg tcccgttcca gaaaaatggt tcgctgaatg tagcatttac
       61 tgaggaggaa accgtgaagc tgcagcagct cctcgaggac ggtttgcgca atggtgtgca
      121 ggggctgaag attttgtcac cgcttgagct tcgtgagctg gagccgcagc tgaatccgga
      181 catccaggcg gctctgtatg ctccgtcgag cggtattacc agccgctcct cgacccagtc
      241 ggcgaggccg tccggggtgg acggcgccca gccgggtagt tcgcgcctga gcctgcggcc
      301 gtaggccgag gcgatcgcgg ggtcgacgcg cgggcgggcg gccgggtcgc tcatcgccct
      361 ggtaatggcc gcctcggcgg ctccggcggc cccgcccgcc ggagtccctg aagaggatat
      421 cgtcgactcg tcgtcctcgt agaggtacct gttcgtctcg gcccagccgc actggcgcgc
      481 gaagggactg ggcgcggccg gcgagaagat cggggcggcg acggagccgt cggccaggcc
      541 cgcgcagagg gccgacagcg aggcgaggtc gaagcgctgc tcgagcaccg tcttccaggt
      601 ctcggcggct atggggaagc cgtcgtagcc gctcgtgcgc tcgaggaggc gcttggcgcg
      661 gagcctcgtg acccagagcg gggtgcgcct gccgaagccg gctcgcggca ggaggagggc
      721 gcgcccggcg ttctccctga aggccgcgcc gaagatagcg ctgccgacga gcgcgccgcg
      781 cagagccctg gacagcgtcc ccgggtcggc gagcgaacgg agcgcgtcca cgagggcgcg
      841 tacggcctcg tcccggtcga cgaggggcat ggacagcgat accgagtcgt cgttcgatag
      901 gcgctcgacc ggcacgccga gcctgtcgcg gagcagcgac gacagggcca agccgagcgg
      961 ctcattgacg gccgagccgc gcagggtatg gacgatcagc gcggtggcgc cggccttggc
     1021 ttccgggtcg cggaaggctt ctataaccac cgagcccggc gaagggagcc tctcaccccg
     1081 ttgggcccgc gactgcgacg acaggaacgc gacgagggcc ttggcggcct cgggcgagaa
     1141 accgagccgg ccgcgtagct cggcggcgag ggcgtccggg tcggcgtcga aggcggcgca
     1201 cagcccgagc atgcggcgag ctatggaggc gccgcgggcg gccttctccg ccttccagaa
     1261 gggcatgaag tcggcgtcgc ggtcgagcgg ggccacggtg accgcctcgt cgcctatgtc
     1321 gacgatccgc cacgcctggg agccgaagct gaagctgttg ccgacgcggc gctcgaagac
     1381 gaactcctcg tcgagctcgc cgagcctcgt accgtcggcc aggcgcatcg cgtagtagcc
     1441 gcggtcgggt atggagcctc cggccgagta gaggagcatc gccgcgcctt ccttggcccg
     1501 gagcgtcccg tgctcctcgt cgaggtaggc gcggccggca agctccctca cgcggctacc
     1561 ggcgaagcgg ccggcgagca tgccgacgac ggcgtcgaag tcggcccgcg cgaggtccgc
     1621 gtagggcggg aaggagcgaa cctcgtcgta tagctcgcca aggcggcgct cgccgaacag
     1681 caccatcgat aggacgacct gggcgagcag gtccaagggc tcgcgcggtg gacgcatcgg
     1741 gtcgatatcc cgatcgaggg cggcgccgac agcggccgcg gcggccagca gatcgaggcc
     1801 gtggaaggga tagacgacgg cgcggctcgt gaggccgacc cggtggccgg agcggccggc
     1861 gcgctggagg agctggtcgg cgcgctgggg cgagccggcg agggcgacca agtcgatagc
     1921 gccgacgtcg atgcctagct cgagcgtgct cgtcgcgacg acacagcgca ggcggccgtc
     1981 cttgagtctg gcctcgacgg ccttgcgtac gtccttggac agggaaccgt ggtgggccca
     2041 ggccgtgccg tcgccggagc gctcgttgag catggccgcg agccgctcgg cccgccggcg
     2101 cgagtcggcg aagacgatgg tcgagcgctc gcgctccagg cgctcggcca gttcgtcggc
     2161 tatcgcgtcg tagcggccgg ccggctcgtc gatcatgcga tcgtcggtct cgcgctcatc
     2221 ggggccggcc gtgggcggcc gtacgcgtaa cggcgccgcc ggccaggcga ccgatagctc
     2281 gtagcgcttg gaggccggcg gcgcgacgac ggccatcgcc ctcggttcgt agcgggcggc
     2341 gccgtccggg ccgcgtacca ggcgcgagcc cgcggcgaag ctggccgcca cgtcgaaggg
     2401 ctcgaccgta gccgataggg cgacgcgctg gaactcg
//
LOCUS       DAOPHV010000426         2428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_61229_length_2428_cov_3.282764, whole genome shotgun sequence.
ACCESSION   DAOPHV010000426 DAOPHV010000000
VERSION     DAOPHV010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61229_length_2428_cov_3.282764"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLQ29_10365"
     CDS             complement(<1..226)
                     /locus_tag="PLQ29_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HPG87090.1"
                     /translation="MSDYLDPNNQELLKDFFAEAYAQVDALEQNILVLENDPANYDAV
                     DEIFRAAHTLKGGAGTVEMTELAGFTHVMED"
     gene            384..1574
                     /locus_tag="PLQ29_10370"
     CDS             384..1574
                     /locus_tag="PLQ29_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87091.1"
                     /translation="MARRYAILLIMALSVASLHGADLSVPRLSLLTNGRLNAVGAFEL
                     ATRVDIDMLVEGGSKFDAWFKLGFRNGSMEQYLRFVGVGSSLPIGATSDDLASAVSGL
                     EASTGLSLRTVAIQVNELFGTSLELAAFVGHLDQFCSGDDFTEAFGADGFATKYRGYF
                     YYPDGIGGDLSRRYDGLHEVYGTGIRLAMPFERLRPSLYLYQDSWLGAGYYSADARVM
                     LDGDRVKLEAFAGASFPVSTAGTYRGGFLFYFDTGAIGDFYAQIGVPRWDPTEAFRMD
                     LLYFMFEPRVRFDVGELVLSLFFHPAWYLQEETDESGALEFRFDLGFGEASEGSFKGG
                     VESELAYNPNLDQNTLTMEVAPYLQTLRNGVRWDARLAVRVFPFPSPWYGMFMPTIAV
                     STAF"
     gene            1594..>2428
                     /locus_tag="PLQ29_10375"
     CDS             1594..>2428
                     /locus_tag="PLQ29_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87092.1"
                     /translation="MTILLIRPLRSARALALAARALALIALAGLMVVPAAALDLEASA
                     FFGNLGLPWAGEEPLTGAQFPSDAWIYGGKAGFTDELGEGLSLYAGYETDPTLRHVVK
                     GIISYESGMASISAGPRVGVFNTAQTPLKAGIEVGFRLDAPGIAFFSVDTASSMGVGL
                     ASAGDYSQEYSDISAGWYVYNAICSFSMTTKRYTRVLASGEPLVDASNDYLFSVDTYK
                     KGAPYRVLTELGYRTMTRSYPDGSSDGLGAVVLAAEISADVSPALTLVAGLDSGVYVF
                     GI"
BASE COUNT          413 a          800 c          760 g          455 t
ORIGIN      
        1 ggtcctccat cacatgcgtg aagcccgcga gttcggtcat ctcaaccgtg ccggcgccgc
       61 ccttgagggt atgggccgcc cggaaaatct catcgaccgc gtcgtagttg gcggggtcgt
      121 tctcgagcac cagaatattc tgctcgagcg cgtccacctg cgcgtaggct tccgcgaaaa
      181 agtccttgag gagttcctga ttgttcggat cgagataatc gctcattata gggagtagtt
      241 tatacgtaac gaaggaatag tcaagagaag taccgtcgcg acggagccgg cggattaatg
      301 agaaagaccg aattctccga tgctttatag gcgaaaccac gctaaattct agacggatca
      361 taccggacga acgggagcga cctatggcga gacgatacgc gattctattg atcatggccc
      421 tgagcgtcgc gtcgctccac ggggcggacc tgagcgtccc tcgcctgagc ctcctgacga
      481 acggcaggct gaacgccgtg ggggccttcg agctggctac tcgcgtcgat atcgatatgc
      541 tcgtcgaggg cggctccaaa ttcgacgcct ggttcaagct cggcttcagg aacggctcga
      601 tggaacagta tctgcggttc gtgggcgtcg gatcgtcgct acccatcggc gcgacaagcg
      661 acgacctggc ctcggcggta tccggcctcg aggcgtcgac cgggctatcc ctgcgtacgg
      721 tcgccatcca ggtcaacgag ctcttcggca cgtcgctcga gctagcggcc ttcgtcggtc
      781 acctcgacca gttctgctcc ggcgacgact tcaccgaggc cttcggggcc gacggtttcg
      841 ccaccaagta ccggggctac ttctactacc cggacggcat agggggtgac ctgagtcgcc
      901 gctacgacgg cctacacgag gtgtacggca ccggcatcag gttggccatg cccttcgagc
      961 gcctcaggcc gagcctgtac ctctatcagg actcgtggct cggcgccggc tactactccg
     1021 ccgacgcccg cgtcatgctc gacggcgacc gcgtcaagct cgaggccttc gccggcgcct
     1081 ctttccccgt atcgaccgcc ggcacctacc ggggcggctt cctgttttac ttcgacaccg
     1141 gggccatcgg agacttctac gcccagatcg gcgtaccgcg ctgggacccg accgaggcct
     1201 tcaggatgga tctcctctac ttcatgttcg agccgcgggt acgcttcgac gtcggcgagc
     1261 tcgtgctcag cctgttcttc cacccagcct ggtacctcca ggaggagacc gacgaatccg
     1321 gagcgctcga gttccgcttc gacctgggct tcggcgaggc ttcggagggt tcgttcaagg
     1381 gcggcgtcga gtctgagctg gcgtataacc cgaacctcga ccagaacacg ctgacgatgg
     1441 aggtagcgcc atatctccag acgctcagga acggcgtccg ttgggacgcg aggctggccg
     1501 tacgggtctt cccgttcccg agcccatggt acggcatgtt catgccgacc atagccgtct
     1561 cgacagcctt ctaggacagc aaggagccac gcgatgacga tactgctgat acgtccgtta
     1621 cgatctgcgc gagcgctggc tctggctgcg cgagcgctgg ctctgatagc gctagctggc
     1681 ctcatggtcg tgccggccgc ggccctcgac ctcgaggcga gcgccttctt cggcaatctg
     1741 ggactgccgt gggccggtga ggagccgttg accggcgccc agttcccgag cgacgcatgg
     1801 atctacggcg gcaaagccgg cttcaccgac gagctcggcg aggggctgtc cctgtacgcc
     1861 ggctacgaga ccgacccgac gctcaggcac gtcgtcaagg gcatcatcag ctacgagtcc
     1921 ggcatggcga gcatcagcgc gggcccgcgc gtcggcgtgt tcaacacggc ccagacgccg
     1981 ctcaaggccg gcatcgaggt cggcttcagg ctcgacgcgc cgggcatagc cttcttctcg
     2041 gtcgacaccg cctcgtcgat gggcgtgggg ctggcgtcgg cgggcgacta ctcgcaggag
     2101 tattccgaca tatcggccgg ctggtacgtc tacaacgcca tctgttcctt ctcgatgacg
     2161 accaagcgct acacccgcgt gctcgcctcc ggcgagccgc tcgtcgacgc gtcgaacgac
     2221 tacctcttct cggtcgatac ctacaagaaa ggcgccccgt accgggtgct caccgagctc
     2281 ggctaccgca ccatgacccg gtcctacccg gacgggagct cggacggcct tggcgcggtc
     2341 gtgctggcgg ccgagatcag cgcggacgta tcgccggcgc tgaccctcgt cgccggcctc
     2401 gactcgggcg tctacgtatt cggcatcg
//
LOCUS       DAOPHV010000427         2427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_61249_length_2427_cov_4.879427, whole genome shotgun sequence.
ACCESSION   DAOPHV010000427 DAOPHV010000000
VERSION     DAOPHV010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61249_length_2427_cov_4.879427"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLQ29_10380"
     CDS             complement(<1..414)
                     /locus_tag="PLQ29_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HPG87093.1"
                     /translation="MDTKFLHATMLLILVTDPLGNIPLFITALERVAPARRTRVILRE
                     VAVAFLILLLAMVGGRAALSVLGIGDASLKAAGGVILLLIAINMIFPAAGARMGEGPT
                     DGEPMIVPIAVPLVAGPSAIATAMLVAADNPGRLPV"
     gene            552..1085
                     /locus_tag="PLQ29_10385"
     CDS             552..1085
                     /locus_tag="PLQ29_10385"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPG87094.1"
                     /translation="MDKGYMHVYTGDGKGKTTAAIGLCVRALGAGMSVYIGQFIKGMR
                     YSEITTLETLAAALGPGRLVVEQYGLGCFIMRAPEKADLEAAAEGLTRAREALASGRY
                     GLVVLDELNVAVQVGVVSEADALAVVDARPPEVELVVTGRYATDAMLGRADLVTEMRE
                     VRHYYRTGVQARTGIER"
     gene            complement(1152..2342)
                     /locus_tag="PLQ29_10390"
     CDS             complement(1152..2342)
                     /locus_tag="PLQ29_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPG87095.1"
                     /translation="MKTNFSVVRELLRKDPALAKKMVHPFVAQKKSMYVDRVLHPATT
                     AELGLIYFRLTPLCNLRCVMCGQRGDKGVLKGSFAAEEAKKIVPLEDYKRLVDQIKHK
                     KPVVYLWGGEPFLYPDLFPLIDYMMEAGLVVSVNTNGTFLEKYAKEIVERKWHALFVS
                     LDGFEETNDRIRGPGSYRRVVDGFEAINREKARQGSHYPYMGVVTTVNNLNYKDLYAL
                     AEATRPFKLAWHIYNLDTYTNDAIVARQREYMKRELDTDIDCLPGYATGYNEGIDGQE
                     LYDILSRVHAMDIDHPIITVPALEPDKIDIYYGKLDEPVRTHCSVPWSQCNIDYNGDV
                     CFCADYNDYPLGNIRETPFFEIFNGERARAFRLALRNAKDGLFPGCIRCYQNMLCGKR
                     VKGF"
BASE COUNT          415 a          754 c          817 g          441 t
ORIGIN      
        1 gacgggcagc ctccccgggt tgtccgcagc gacgagcatc gcggtcgcta tcgccgacgg
       61 cccggcgacc agcggcacgg ctatcggcac gatcatgggc tcgccgtcgg tcgggccctc
      121 gcccatgcgc gcgccggcgg cggggaagat catgttgatc gcgatgagaa ggagtatcac
      181 gccgccggcc gccttgaggc tggcgtcgcc tatgccgagc accgacaggg cggcccggcc
      241 gccgaccatc gccagtaaga ggatcaggaa cgcgacggcc acctcgcgca gtatcacgcg
      301 ggtgcgccgc gccggcgcaa cgcgctcgag cgccgtgatg aacagcggaa tgttgccgag
      361 cgggtcggtc acgagaatca gcaacatcgt cgcgtgcagg aacttggtat ccatggagcc
      421 tccgtgaacc tagcctcgag tatacaagag accgcggcgt cgatggaaca gtggccgatg
      481 gccgctccct cgatcttgac gccggagcct cgttgccgta gggtgcccgg tatcgatcgg
      541 gaggcgctgc gatggacaag ggctacatgc acgtgtatac gggcgacggc aagggcaaaa
      601 cgacggcggc cataggcctc tgcgtacggg cgctcggcgc cggtatgtcc gtctatatcg
      661 ggcagttcat caagggcatg cgctactcgg agataacgac gctggagacg ctggccgccg
      721 ctctcggccc cgggcgcctc gtcgtcgagc agtacgggct cggctgcttc atcatgaggg
      781 cgcccgagaa ggccgacctc gaagccgccg ccgaaggcct gacgcgggcc cgcgaggcgc
      841 tcgcctccgg ccgctacggc ctcgtggtcc tcgacgagct caacgtcgcg gtccaggtcg
      901 gcgtcgtatc cgaggcggac gcgctcgccg tcgtcgacgc ccggccgccc gaggtcgaac
      961 tcgtcgtcac cggccgctac gcgaccgacg ccatgctggg gcgcgccgac ctcgtcaccg
     1021 agatgcgcga ggtcaggcac tactaccgga ccggggtgca ggcgcgtacg ggcatcgagc
     1081 gatgaggcta ccgcgcttcg ccataggcgc gctccacgcc cgaaggctgg tcgcggcggc
     1141 ccggcgttct cttaaaaacc cttcacccgc ttaccgcaca gcatattctg ataacaacgg
     1201 atacaacccg ggaataggcc gtccttcgcg ttcctgaggg ccagcctgaa cgcgcgggcc
     1261 cgctcgccat tgaatatctc gaagaacggc gtctcgcgga tgttgccgag cggatagtcg
     1321 ttgtagtcgg cgcagaagca gacgtcgccg ttgtaatcga tgttgcactg gctccagggc
     1381 accgagcagt gcgtgcgcac cggctcgtcc agcttgccgt agtagatatc gatcttgtcc
     1441 ggctcgagcg cgggcacggt gatgatcggg tggtcgatgt ccatcgcgtg gacgcgggac
     1501 agaatgtcgt acagctcctg gccgtcgatg ccttcgttgt agccggtggc gtagccaggc
     1561 aggcagtcga tatcggtatc gagctcccgc ttcatgtact cgcgctgcct ggcgacgatg
     1621 gcgtcgttcg tatacgtgtc caggttgtag atatgccagg ccagcttgaa gggcctcgtc
     1681 gcctcggcca gagcgtacag gtccttgtag ttgaggttgt tgacggtggt gacgacgccc
     1741 atgtacgggt agtgcgaacc ctgcctggct ttctcgcggt tgatcgcttc gaagccgtcg
     1801 acgacgcgac ggtacgagcc ggggccccgg atgcggtcgt tcgtctcctc gaagccgtcg
     1861 agcgagacga acagcgcgtg ccatttgcgc tccacgatct ccttggcgta tttttcaagg
     1921 aacgtgccgt tggtgttgac gctgacgacg aggccagcct ccatcatgta gtcgatcaag
     1981 gggaagaggt cggggtacag gaagggctcg ccgccccaga ggtagacgac cggcttctta
     2041 tgcttgatct ggtcgacgag gcgcttgtag tcctcgagcg gcacgatctt cttggcctcc
     2101 tcggccgcga aggaaccctt gagcacaccc ttgtcgccgc gctggccgca catgacgcag
     2161 cgcaggttgc atagcggcgt cagccgaaag tagatcaacc cgagctcggc cgtcgtggcc
     2221 gggtgcagga ccctgtcgac gtacatcgac ttcttctggg ccacgaaggg gtgtaccatc
     2281 ttcttggcga gggcgggatc cttccggagc agttcgcgaa cgaccgaaaa attggttttc
     2341 atcgtgagtc catcctggtt gcgtggtgtt cgttagacga gtctatcgag gaatagtttc
     2401 atgattgcag gcggcgcggt ctgaggc
//
LOCUS       DAOPHV010000428         2427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_61252_length_2427_cov_4.559022, whole genome shotgun sequence.
ACCESSION   DAOPHV010000428 DAOPHV010000000
VERSION     DAOPHV010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61252_length_2427_cov_4.559022"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="PLQ29_10395"
     CDS             complement(<1..130)
                     /locus_tag="PLQ29_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967890.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HPG87096.1"
                     /translation="MDFFLKLFLSGTAKGSIYALIALGYTMVYGIIELINFAHGEIY"
     gene            complement(216..1337)
                     /locus_tag="PLQ29_10400"
     CDS             complement(216..1337)
                     /locus_tag="PLQ29_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HPG87097.1"
                     /translation="MRRSMVVVAILAVSVALILSGCQKSASGTIKIGVAGAHSGDLAS
                     YGLPSVNAAKLVVEAVNAAGGIDGKTVELFIQDDQCKPELATNAAAKLVSDEVVAVIG
                     HICSGATKSALGIYKDSKIITVSPSATTPSLTQSGEYPNFFRTIAPDDAQARLATKFI
                     KETLGLSSLAVLHDKGDYGKGFAELVKQYAESEGIRVVLFEGINPGAPDYSAVVNKIG
                     NSGAAGVVWGGYHPEASKLVQQMKDKGMSLPFISDDGVKDQTFIEVAGTYAEGVYATG
                     PMDTSSNPLAVKAVADHKAKFGDEPGAFFLNAYAATLAVVNAIDKAGSADYDAVVKAL
                     RSEYVETPLGRISFDGIGDVIGFGFSVFQVQGGVYVELK"
     gene            1586..>2427
                     /locus_tag="PLQ29_10405"
     CDS             1586..>2427
                     /locus_tag="PLQ29_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HPG87098.1"
                     /translation="MKLSFGEKASYGVGALGKDMACGIVNTFLMFYFTDVIGLAPAFL
                     GVLFMAARIWDAINDPMAGWLVDNTRGRLGKFRPWILAGTLSNAVVLVVLFSNPGLRG
                     AGQYVFYSVVYILWGMTYTLEDISYWSMIPALADDEGERNTISVIPRVFAAVGNIAVG
                     SLGLALVARLGGVAPDAMPTPEQQSRGFFWLAVLIGAAFVVTNVVTVLFVKEKVVVPS
                     DRKFKFRDIFTTIAGNDQLLVILVAMTLYTLAINLTASLGLYWFKYDVGDEGLYGTFY
                     LTAAA"
BASE COUNT          431 a          765 c          764 g          467 t
ORIGIN      
        1 tgtagatctc gccgtgggcg aagttgatga gctcgatgat gccgtagacc atggtatagc
       61 cgagggcgat gagcgcgtag atgctaccct tggccgtgcc gctcaggaaa agcttaagga
      121 aaaaatccat gatgacccct tctcggccgg agcgcccata cggaagcggc cgccccgacg
      181 ccggggcggc cgcggggccg ctctatcgcc gacgcctact tcagctcgac gtagactccg
      241 ccctggacct ggaacacgga gaagccgaag ccgatgacgt cgccgatgcc gtcgaagctg
      301 atcctgccga gcggcgtctc gacgtattcg gagcgcaggg ccttgaccac cgcgtcgtag
      361 tcggcggagc cggccttgtc tatcgcgttg accacggcga gcgtggccgc gtacgcgttc
      421 aggaagaagg cgcccggctc gtcgccgaac ttggccttgt ggtccgccac cgccttgacc
      481 gccagcgggt tggagctggt atccatcggg ccggtcgcgt agacgccctc ggcgtaggtt
      541 ccggccacct cgatgaaggt ctggtccttg acgccgtcgt cggagatgaa cggcaggctc
      601 atgcccttgt ccttcatctg ctgaacgagc ttggaggcct cgggatggta gccgccccag
      661 acgacgccgg cggcgccgga gttgcctatc ttattgacga ccgccgagta gtccggcgcg
      721 ccggggttga tgccctcgaa gagcacgacc cggatgccct cgctctcggc gtactgcttg
      781 acgagctcgg cgaagccctt gccgtaatcg cccttgtcgt gcagcaccgc gagcgacgag
      841 agcccgagcg tctccttgat gaacttggta gccagcctgg cctgggcgtc gtccggagct
      901 atggtcctga agaagttagg gtactcgccg ctctgggtca gcgacggcgt ggtcgcggac
      961 ggggagacgg tgatgatctt cgaatcctta tagatgccga gcgccgactt ggtggcgccg
     1021 gagcagatat ggccgatgac ggcgacgacc tcgtcgctga cgagcttggc ggcggcgttg
     1081 gtcgccaact cgggcttgca ctggtcgtcc tggatgaaca gctcgaccgt cttgccgtcg
     1141 atgccgccgg ccgcgttcac cgcctcgacg accagcttgg ccgcgttcac cgaaggcagg
     1201 ccgtaggaag ccaggtcgcc cgagtgcgcg ccggccacgc cgatcttgat ggtcccgctc
     1261 gcgctcttct ggcagccact aagaatgagc gcgactgata ctgccaggat cgccactacc
     1321 accatgcttc ttctcattcg ttcctcctaa tcagctgccg catcgtacgt ttatgaccac
     1381 cgcggtcatc gcgccgacga agcgccaggt acaagtgttc ctatcgtttc tatgtatagt
     1441 agtagcgtcg ccttagtcaa gtagcgaatg cctatgcata gaatcaaaca tgccaggagt
     1501 cggcgcaagg tacgcattga cgatacgacc ggccgcgccg atactgttca ggccgcccgg
     1561 atcgcgcggc cttcaaggag actgcatgaa actgtctttc ggagaaaaag cgagctacgg
     1621 cgtcggcgcg ctcggcaagg atatggcctg cggcatcgtc aacaccttcc tcatgttcta
     1681 ttttacagac gtgatagggc tggccccagc gttcttaggc gttttgttca tggcggccag
     1741 aatctgggac gcgatcaacg acccgatggc cggctggctg gtcgataaca cgagaggccg
     1801 gctcggcaaa ttcagaccct ggatactcgc cggcacgctg agcaacgccg tggtcctcgt
     1861 cgtgctgttc tcgaacccgg gcctccgcgg cgccgggcag tacgtcttct acagcgtcgt
     1921 ctacatactt tggggcatga cctatacgct cgaggacatc tcgtactggt cgatgatacc
     1981 ggccttggcc gacgacgagg gcgagcgcaa cacgatatcg gtcataccgc gcgtgttcgc
     2041 ggcggtcgga aacatagccg taggttcgct cggcctagcc ctcgttgcga ggctcggcgg
     2101 cgtggccccg gacgccatgc cgacgccgga gcagcagtcg cgcggcttct tctggctcgc
     2161 cgtgctgatc ggcgcggcct tcgtcgtaac gaacgtcgtc accgtgctgt tcgtcaagga
     2221 aaaggtggtc gtgccgagcg accggaaatt caagttcagg gacatattca cgacgatagc
     2281 cggaaacgac cagctcctcg tcatcctcgt cgccatgacc ctgtacacgc tcgcgatcaa
     2341 cctgaccgcc agcctcggcc tgtactggtt caagtacgac gtgggcgacg agggactgta
     2401 cggcaccttc tacctgacgg ccgccgc
//
LOCUS       DAOPHV010000429         2424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_61342_length_2424_cov_4.790629, whole genome shotgun sequence.
ACCESSION   DAOPHV010000429 DAOPHV010000000
VERSION     DAOPHV010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61342_length_2424_cov_4.790629"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="PLQ29_10410"
     CDS             <1..1439
                     /locus_tag="PLQ29_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87099.1"
                     /translation="GASVKFVKNCEARFFQRPDDAIHRGYDKQAEADMARPDNFISNF
                     EPLSRAKAKEMVERTVELSEYTEPMREMIVGAESDDRFRWFVVSSKPRLVDGKPSKNP
                     RYLQLDRNYAEPLERYLADVGARLFRMVKSDAPVYYPVGAILPGRRNNPPEPEAGIRP
                     LAVYGPVHYQELPELFMDFICSLTGKSPSTTGAGSEGALTKGPFNSLTPTSDLNNALL
                     GFILGGYDGFSTAAGYIGHKYRVEHDISLLVPELWARLTPEERDPAFLIESGYLERVE
                     DFEHGGRTVPAGRLGWRVTPLFASHFLGRIFDAPSSVFPEEVLRPELQSLEYFADGVL
                     NIAEAQERVARDYLQDGSIEAAIPPLQAILRVMADGSWNGKGLDDPELRRLFDRDYVL
                     ASDWYAARLEAYAEAERSRLREGVAYLEEFLGSPRGRDERDLAKARRELTTVQQSLER
                     ASRPEYAASLVGTIGVDPLFRSGLGAGR"
     gene            1436..>2424
                     /gene="hutI"
                     /locus_tag="PLQ29_10415"
     CDS             1436..>2424
                     /gene="hutI"
                     /locus_tag="PLQ29_10415"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPG87100.1"
                     /translation="MRATLLIENIGELATPVGSTARQGAVMRELRIVRDAVVAVDVET
                     IAWAGPASELPRLEGPPPARLDARGRAVVPGFVDSHTHFVFGGYREDEFFWRAEGLPY
                     MEIHARGGGIANSVRDTRAASAEELTAAAAARLERMLELGVTTVEGKSGYGLDLETEL
                     RQLEVMRLLDSRQPVDIVSTYMGPHSVPAEFKGNPSGYIDFVLREVMPRVKEGGLARF
                     CDVFCERGVFELDDSRRFLEAAKSMGFGVKLHADEIVRLGGAGLAAEIGAVSADHLLK
                     ASEDDLLAMAEAGVVATCLPLTAFSLREPYAPARAMIDRGLAVAVASDLNPGS"
BASE COUNT          396 a          841 c          797 g          390 t
ORIGIN      
        1 gcggggcgtc ggtgaaattc gtcaagaact gcgaagccag gttcttccag cggcccgacg
       61 acgccatcca tcgcggctac gacaaacagg ccgaggccga tatggcgcgg ccggacaact
      121 tcatctcgaa cttcgagccg ctgtcgcggg ccaaggccaa ggagatggtc gaacgcaccg
      181 tggagctgtc cgagtacacc gaacccatgc gcgagatgat agtcggcgcc gagagcgacg
      241 acaggttccg ctggttcgtc gtctcgtcca aaccccggct cgtggacggc aagccgtcga
      301 agaacccgcg ctacctgcaa ctggaccgca actacgcgga gccgctcgaa cgctacctgg
      361 cggacgtcgg ggccaggctg ttccgcatgg tcaagtccga cgcgccggtc tattacccgg
      421 tcggtgccat cctgcccggc aggcgcaaca acccgcccga gccggaggcg ggcatcaggc
      481 cgctggccgt ctacggcccg gttcattatc aggaactgcc cgagctgttc atggacttca
      541 tctgctccct gaccggcaag agcccgtcca cgacgggggc cggatccgag ggagcgctga
      601 ccaagggccc gttcaactcg ctgacgccga cctccgacct gaacaacgca ctgctcggct
      661 tcatactcgg gggctacgat ggtttctcga cggccgccgg atacataggc cacaagtacc
      721 gcgtcgaaca cgacatctcg ctgctcgtgc ccgagctgtg ggccagattg actcccgagg
      781 agcgcgaccc ggccttcctg atcgagagcg gctacctcga gcgcgtcgag gacttcgagc
      841 atggcggccg aaccgtaccc gcgggccgcc tcggctggcg cgtcacaccg ctgttcgcgt
      901 cccacttcct cggccgcatc ttcgacgccc catcgtcggt gttccccgag gaggtgctcc
      961 ggcccgagct gcagagcctc gagtacttcg ccgacggcgt gctcaacata gccgaggccc
     1021 aggaacgcgt cgctcgcgac tacctccagg atggttcgat cgaggcggcc atcccgccgc
     1081 tccaggccat cctgcgcgtg atggcggacg gttcctggaa cggcaagggt cttgacgacc
     1141 ccgagctacg gcgcttgttc gatcgcgact acgtacttgc gtccgactgg tacgccgctc
     1201 gcctcgaggc ctacgccgag gccgagcgat cgaggctacg cgagggcgtc gcctaccttg
     1261 aggaattcct aggctcgcct cgcggccgtg acgagcgcga cctcgcgaag gcccgtcgcg
     1321 agctgacgac cgtccagcag agcctcgagc gcgcgtcgcg gcccgagtat gccgcctctc
     1381 tcgtaggcac gatcggcgtc gacccgctgt tccgctccgg actgggagcc ggacgatgag
     1441 ggcgacgctg ctgatcgaga acatcggcga gctcgcgacg cccgtcggtt cgactgcgcg
     1501 ccagggcgcc gtcatgcgcg aactacggat agtccgcgac gcggtcgtcg ccgtcgatgt
     1561 ggagacgatc gcctgggccg gtccggcctc cgagctaccg cggctcgagg ggccgccgcc
     1621 ggcccgcctc gacgcgcgcg gccgagccgt cgtgccgggc ttcgtcgact cgcacacgca
     1681 tttcgtcttc gggggctacc gcgaagacga gttcttctgg cgggccgagg gcctgccgta
     1741 catggagatc cacgcgcgcg gaggcggcat cgccaactcg gtgcgcgata ccagggcggc
     1801 ctcggccgaa gagctgacag ccgccgccgc cgctcgcctc gagaggatgc tcgagctcgg
     1861 cgttaccacc gtcgagggca agtctggcta cggcctcgac ctggagactg agcttcgtca
     1921 gctcgaggtg atgcggctac tcgactccag gcagcccgtc gatatcgtat cgacttacat
     1981 gggtccgcat tccgtcccgg ccgaattcaa gggaaacccg tccggctaca tcgacttcgt
     2041 cctacgcgag gtcatgcccc gggtcaagga aggcggcctg gcgcgtttct gcgacgtctt
     2101 ctgcgaacgt ggagtcttcg aactcgacga ctcgaggcgc ttcctcgagg cggccaagtc
     2161 catgggcttc ggcgtcaagc tgcacgccga cgagatcgtc aggctcgggg gagccgggct
     2221 agccgccgag atcggcgcgg tcagcgccga tcaccttctc aaggcttccg aagacgatct
     2281 cctggccatg gcggaggccg gcgtcgtggc gacctgcctg ccacttacgg cgttcagcct
     2341 acgggagccg tacgcccccg ccagggcgat gatagaccga gggctggcgg tcgcggtcgc
     2401 gtcggacctc aatcccggct cgtg
//
LOCUS       DAOPHV010000430         2405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_62048_length_2405_cov_4.764255, whole genome shotgun sequence.
ACCESSION   DAOPHV010000430 DAOPHV010000000
VERSION     DAOPHV010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62048_length_2405_cov_4.764255"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1641)
                     /locus_tag="PLQ29_10420"
     CDS             complement(<1..1641)
                     /locus_tag="PLQ29_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPG87101.1"
                     /translation="MRIDELPIFDLLPDIADAIAKGRLALSAETGAGKTSAVPAYLAA
                     ERTLPGKTLVLEPRRLAAVAAAARVAELLGSPLGEAAGYRVRGDSRAGRRTIVEYVTD
                     AVFIRMAQDDPFLSGVSAVILDEFHERSAYGDLALAFALEAAEAREGLSVVAMSATMD
                     AAAVAAYLGGPAIAAPGRAYPVDVGYRPPKPDERVEDAVARAIVEALESTDGDVLAFL
                     PGLREIEAVRASLGRRSDGSAARARRGCAPDILTLHGSMPLVEQRRVTLPPEGASRRV
                     ILATNVAETSLTVPRITAVVDSGLSRLSRFHAPSGLNRLVTERASLAETDQRKGRAGR
                     LGPGLCLRCWDGADILAPSRGPELARTELSAVALECAVRGSSRLEATRWLDEPPRHMW
                     DAAVETLGRIGLLDSGGRATESGRTAAALGAEPRAAAALLAAAAGDRPDDTHAAALAA
                     AALTERGDRDDDGDYRSGLERLLEAARRGRVDDRGARILREAGRLLSRLPGGPAFSAE
                     RASRALESVGELLAPGFPDRLARRLADDSWEFPSGRRAR"
     gene            1687..>2405
                     /locus_tag="PLQ29_10425"
     CDS             1687..>2405
                     /locus_tag="PLQ29_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87102.1"
                     /translation="MDDAWDPEARSRVAAESPGIGLYAERGLHAALKDRYAREPGARV
                     EARVAGKVVDVVLPDELVEVQTRNLGSIAEKILHLACVMPVRLVHPIVVENLIVRVDP
                     ADGSAGAPRRSRTRRDFYSLFDELVRAPLLVASPNVRLEVVLVRVRELRVRDGTGSWR
                     RRGDRVLSRDLDEVLGVTSLDGRDDWLAIIPPGLAQPYDSASLGDALGVAAARARKIL
                     YAYAKAGLLEEAGKSGNRKLYR"
BASE COUNT          317 a          896 c          877 g          315 t
ORIGIN      
        1 tctggcccgc cgtccggacg ggaactccca gctgtcgtcg gccaggcgcc gcgccaggcg
       61 gtcagggaag ccgggcgcca ggagctcgcc cacggattcc agggcccgcg atgcgcgttc
      121 ggcgctgaaa gccggtccgc caggcagcct cgacaggagg cgcccggcct ccctcagtat
      181 cctcgcgccg cggtcgtcga cccggccgcg cctggccgcc tcgagcaggc gctcgaggcc
      241 gctcctgtag tcgccgtcat cgtcccggtc gccgcgctcg gtcagcgcgg ccgcggccag
      301 ggccgccgcg tgcgtgtcgt cggggcgatc gccggccgcc gccgctagca gcgcggcggc
      361 ggcccggggc tcggcgccga gcgccgcggc cgtcctgcct gactccgtgg cccggccgcc
      421 cgagtcgagg aggccgatgc gcccaagcgt ctcgacggcg gcgtcccaca tatgccgcgg
      481 cggctcatcg agccagcgcg tcgcttccag gcgcgaagat ccgcggaccg cgcattcgag
      541 cgctacggcg gatagttccg tcctggccag ctccggtccc cgtgacggcg cgaggatgtc
      601 agccccgtcc cagcagcgca ggcagaggcc gggcccgagg cggccggcgc ggcccttgcg
      661 ctggtcggtt tccgccaggg acgcgcgctc ggtcacgagc cggttgaggc cgctcggggc
      721 gtggaagcgc gacagcctgg ataggccgga gtcgacgacc gccgtgatcc tcggcaccgt
      781 aaggctcgtc tcagccacgt tcgtcgccag gatgacccgc cggctcgctc cctccggagg
      841 cagcgttacc cgacgctgct cgacgagcgg catcgagccg tggagcgtca ggatatccgg
      901 cgcgcagcct cggcgggccc gagcggccga gccgtccgac cggcggccga gcgaagcgcg
      961 cacggcctcg atctcacgga ggccgggcag gaaggcgagc acgtcgccgt cggtcgactc
     1021 gagcgcctcg acgatagccc gggccaccgc gtcctcgacg cgctcgtcgg gcttcggcgg
     1081 gcggtatccg acgtcgacgg ggtaggcgcg gcctggggcc gcgatggccg gcccgccgag
     1141 gtaagccgcg accgcagcgg cgtccatcgt cgccgacatg gccacgaccg acagcccctc
     1201 gcgagcctcg gccgcctcca gcgcgaaggc cagggccagg tcgccgtagg ccgagcgctc
     1261 atggaactcg tcgaggatca cggccgagac gcccgagagg aaggggtcgt cctgcgccat
     1321 gcggatgaac accgcgtcgg tgacgtactc gacgatcgtg cgtcggcccg cccgcgagtc
     1381 gccccgtacg cggtagccgg cggcctcgcc gagtggcgag ccgagcagtt cggcgacgcg
     1441 ggccgccgcc gcgaccgccg cgagccggcg tggctcgagc accagggtct tgccgggcaa
     1501 ggtccgttcc gcggccaggt aggccggcac cgcgctcgtc ttgcccgcgc cggtctccgc
     1561 cgacagggcc aggcgcccct ttgctatggc gtcggctatg tccggcagca ggtcgaatat
     1621 gggcagctcg tcgatgcgca tcgcccgcat tgtgccctaa gccgaggcgc gtgtatactg
     1681 aggcccatgg atgacgcatg ggatcccgag gcgcgtagtc gggtcgccgc cgaatccccg
     1741 ggcatcgggc tctacgccga gcgaggtctc cacgccgcgc tcaaggaccg ctacgcccgt
     1801 gagcccggcg ccagggtcga ggcccgggtg gccggcaagg tcgtcgacgt ggtcctgccc
     1861 gacgaactcg tcgaggtgca gacccgaaac ctcggctcga tagccgagaa gatactccac
     1921 ctggcctgcg tgatgcccgt ccgtctcgtc catccgatcg tcgtcgagaa cctcatcgtg
     1981 cgcgtcgacc ccgccgacgg ctcggccggc gcgccgaggc gctccaggac cagacgcgac
     2041 ttctacagcc tgttcgacga gctcgtgcgc gctccgctcc tcgtcgcctc gccgaacgtg
     2101 cggctcgagg tggtgctcgt gcgcgtgcgg gagctgcgcg tgcgcgacgg cacgggttcc
     2161 tggcgccgcc gaggcgaccg cgtgctgtcc cgcgacctcg acgaggtgct cggcgtgacc
     2221 agcctggacg gccgcgacga ctggctggcc atcatcccgc ccggcctggc ccagccctac
     2281 gattcggcgt cgctcggaga cgcgctcggc gtcgcggcag cgcgggcgcg caagatactc
     2341 tacgcctacg cgaaggccgg cctcctcgag gaggccggga agagcgggaa tcgcaagcta
     2401 tatcg
//
LOCUS       DAOPHV010000431         2398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_62314_length_2398_cov_5.311140, whole genome shotgun sequence.
ACCESSION   DAOPHV010000431 DAOPHV010000000
VERSION     DAOPHV010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62314_length_2398_cov_5.311140"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..818
                     /locus_tag="PLQ29_10430"
     CDS             183..818
                     /locus_tag="PLQ29_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPG87103.1"
                     /translation="MAIRYHTATFENIPSEKRERILRAASRVLGRDGIGGARMADVAA
                     EAGVSHGSMFSYFPTKDDMIRAVIARGMSLQAERFAASDDEGAGFAATVRTVFRSAWD
                     EASAEPELISLWLSLSLVENARFADAILPLERDSTERWRRVVARGKASGEIDQGIDDG
                     AAAYLLDALAAQLMKSRASGLERAKLELFVGTGLAEAAPDRMTDAVVAMLR"
     gene            894..>2398
                     /locus_tag="PLQ29_10435"
     CDS             894..>2398
                     /locus_tag="PLQ29_10435"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HPG87104.1"
                     /translation="MDRFSDGFLFGASSSAFQIEGSLDRDGRGPSVWDAFIARPGRER
                     LRSDAATACDHYARWADDVAIAGELGLDAYRFSVSWPRIQPDGRGEPNRAGLDFYSRL
                     VDGLLERDVRPFPTLFHWDLPLALQRDSGGFRDRDTAARFEDYAGIVARALGDRVKDW
                     ITINEPFEYACFGHLFGTHAPGVKSPLAYLQVVHHQLLAHGRAVSAIRSEAAGARVGI
                     ALSWTPVHPLRDTEADEKAAARANAFMNGVSFDPILLGRYPALVTRGLLRPPVRDGDF
                     ETISTPLDFVGLNYYSREKARNAPLVPFIRADVTGKEPRELPESDDRTAMGWEVYHRG
                     LVEILAELRGRYGNPPVIVTEFGSAWSDSVETDAATGRARVGDTRRVRYLRDALAAMR
                     SAIEAGSDLRGAFVWSLTDNFEWAEGNAKRFGLVHVDYGTQARTVKDSGRWYAGLIAS
                     RVLDASSRVLDASSRVLDASSRVLDASSRVLDASSRVLDASSRVLDASSRVL"
BASE COUNT          382 a          841 c          779 g          396 t
ORIGIN      
        1 ccagcatacc tccctgaatt cgacagcccc gccgtcgatc ccggccgctc gcatccgttc
       61 catcgctacc tctctgattg gttagcgact atagcaccaa gtccttacgc gtatccacca
      121 ataaggttcg taacactgac cgcgccttga ccgctcaggc cgcttgcgac gatactcgcc
      181 ccatggcaat ccggtatcat accgccacct tcgagaacat tccctcggag aagcgcgagc
      241 gcatcctccg ggccgcctcg cgcgtgctcg gccgcgacgg catcggcggc gcgagaatgg
      301 cggacgtcgc cgccgaggcc ggcgtctccc atggctccat gttcagctat ttcccgacca
      361 aggacgatat gatccgcgcg gtcatcgccc ggggcatgag cctgcaggcc gagcgcttcg
      421 cggcgtcgga cgacgaaggg gcaggcttcg ccgcgacggt ccgcaccgtc ttcaggagcg
      481 cctgggacga ggcgagcgcc gagcccgaac tcatatcgct ctggctgtcc ctatcgctcg
      541 tcgagaacgc ccgcttcgcc gacgccatcc tgccgctcga gcgcgactcg accgagcgat
      601 ggcgccgcgt cgtcgcccgt ggcaaggctt ccggcgagat cgaccagggg atcgacgacg
      661 gcgcggccgc ctacctcctc gacgcgctgg ccgcccagct gatgaagagc cgcgccagcg
      721 ggctcgaacg cgccaagctc gagctgttcg tcggcacggg gctggccgag gccgctcccg
      781 accgtatgac cgacgccgtc gtcgccatgc tacgatagcc ccattctaat gattgactgg
      841 tcagtcaacg catggtagcc tagcgtcagc ttgatgacga ggagtatcgg cgtatggatc
      901 ggttcagtga cggctttttg ttcggggctt catcgtcggc gttccagatc gagggaagcc
      961 tcgaccgcga cggccgcggc ccgtccgtct gggacgcctt catagcgagg ccgggccggg
     1021 agcgcctgcg ttccgatgcc gcgacagcct gcgaccatta cgccagatgg gccgacgatg
     1081 tcgccatagc cggcgagctc gggctcgacg cgtatcgctt ctccgtatcg tggccgcgga
     1141 tccagccgga cggccgcggt gaaccgaatc gggcgggcct ggacttctac tcgcgcctcg
     1201 tcgacggcct cctggagcgg gacgtgcggc ccttcccgac gctgttccac tgggatctgc
     1261 ccttggcgct ccagcgcgac tcgggcggct tcagggaccg cgatacggcc gcccgcttcg
     1321 aggactacgc cggtatcgtc gctcgcgcgc tcggcgaccg ggtcaaggat tggatcacca
     1381 tcaacgagcc cttcgagtac gcgtgttttg gccacctctt cggtacccac gcaccgggcg
     1441 tcaagagtcc cctggcctac cttcaggtcg tccatcatca gctactggcc catggccggg
     1501 ccgtctccgc catacgctcg gaggccgccg gcgcccgggt cggcatagcc ctcagctgga
     1561 cgccggtgca tcccctacgc gataccgagg cggacgagaa ggccgccgcc cgagccaacg
     1621 cgttcatgaa cggcgtcagc ttcgacccaa tcctgctcgg gcgctatccc gccctcgtga
     1681 cgagggggct actcaggccg cctgtcaggg acggcgactt cgaaaccatc tcgacgccgc
     1741 tcgatttcgt cggcctgaac tactacagtc gggaaaaggc gcgcaacgct ccgctcgtgc
     1801 cgttcatccg ggcggacgtg accggcaagg agccgcgcga gctgcccgag agcgacgata
     1861 ggaccgcgat gggctgggag gtctaccacc gggggctcgt cgagatcctg gccgagctgc
     1921 gcggccgcta cgggaacccg ccggtcatcg tcaccgagtt cggctcggcc tggagcgact
     1981 cggtcgagac ggacgcggct acgggccggg ccagggtcgg cgacacgagg cgcgtccgct
     2041 acctgcgcga cgcgctcgcc gcgatgcgct cggccatcga ggccggctcg gacctgcgcg
     2101 gcgccttcgt ctggagcctg accgacaact tcgagtgggc cgagggcaac gccaagcgct
     2161 tcggcctggt acacgtcgac tacggcacgc aggcccgtac cgtgaaggac tcgggccgct
     2221 ggtacgccgg gctcatcgcg tcgagagtac tcgacgcgtc gtcgagagta ctcgacgcgt
     2281 cgtcgagagt actcgacgcg tcgtcgagag tactcgacgc gtcgtcgaga gtactcgacg
     2341 cgtcgtcgag agtactcgac gcgtcgtcga gagtactcga cgcgtcgtcg agagtact
//
LOCUS       DAOPHV010000432         2393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_62491_length_2393_cov_5.140719, whole genome shotgun sequence.
ACCESSION   DAOPHV010000432 DAOPHV010000000
VERSION     DAOPHV010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62491_length_2393_cov_5.140719"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PLQ29_10440"
     CDS             <1..1044
                     /locus_tag="PLQ29_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87105.1"
                     /translation="SAGDPPVDHRVAGAVAGLSFLASCVPEPALERFSADDLRPPAVA
                     SWGPIGPSEVSIGFDEDIAGAEPGFVSYPGPAVASVALGEDGRSVRVLLDGAQAPGVA
                     YSVSGVVADAAGNLSGFALPYWGHNPDPPAMLINELVTEGSATHPDAVEFYVARGGDC
                     AGLAFFVGSPMDYACRYVFPPLAVAAGDYVVLHLKPQGVAGEVDESTAKDESAGLDAS
                     ASAWDLWCRGGDGALPGKNGVVALCSSPHGAMLDAVPYSERFADSDTAYGGFGSAALR
                     DRVAAVVAAGAWVGSDPPRPEDCARSAGTTSTRSVCRSSSSGDSDSGADWHVVPTKGA
                     SLGSANADEVYAP"
     gene            1094..1624
                     /locus_tag="PLQ29_10445"
     CDS             1094..1624
                     /locus_tag="PLQ29_10445"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPG87106.1"
                     /translation="MEKRSYRELGVALVGLPASGKTSSGVLLADLLGMPFVDLDERVE
                     REAGRSIPALFEDEGEDGFRRRERVALEHVAAGVAAGEPVVLATGGGTVELPENRSVL
                     RERFVTVWIKVDPAAAAARSLGSGRPLLSGDVEAGLRRLYERRRAWYDECAAIAVQSD
                     GMRPAMLAEAIREALR"
     gene            1608..>2393
                     /locus_tag="PLQ29_10450"
     CDS             1608..>2393
                     /locus_tag="PLQ29_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87107.1"
                     /translation="MKRSVSPSMVAGRVVAPPSKSAMQRAVACAAMADGVSTITNPSL
                     CADAIAACRVAEALGASVTRYDDRVVVRGGLSAAPGIVVASCGESGLCLRMFSAIAAL
                     LPVDVELRAEGSLASRSVAMVADALEALGVRCSTSGGLPPVRLRGPLRPGRVRVDGSA
                     SSQFVTGLVMALAMAGGESTVSVERLASGGYVELTFEVMRAFGAEAERDGTEIRIAGT
                     PYRAAEYEVEGDWSGAAFMLVAGAVAGRGDLEVVGLRHGSSQPD"
BASE COUNT          280 a          848 c          892 g          373 t
ORIGIN      
        1 agcgcgggcg atcctcccgt cgaccatcgc gtcgcgggcg ccgtcgccgg gctgtcgttc
       61 ctggcctcgt gcgtaccgga gccggcgctg gaacggttct cggccgacga cctccggccg
      121 ccggccgtgg cgtcctgggg gcccatcggc ccgtccgaag tatcgatcgg cttcgacgag
      181 gacatcgccg gggccgaacc cggcttcgtc tcgtatccag ggccggcggt ggcgtcggtc
      241 gccttgggcg aagacggccg ttcagtcagg gtgctgctcg acggcgccca ggcgccgggc
      301 gtggcctact cggtgtccgg cgtcgtcgcc gacgcggcgg gcaacctgtc cggcttcgcg
      361 ctgccgtact ggggccacaa tccggaccca ccggccatgc tgatcaacga gctcgttacc
      421 gagggctcgg cgacccaccc ggacgcggtc gagttctacg tggcgcgggg cggcgactgc
      481 gccggtctgg ccttcttcgt cggctcgccg atggactacg cctgccgcta cgtgttcccg
      541 ccgctcgccg tggccgccgg agactacgtc gtgctgcacc tgaagcctca gggcgtggcc
      601 ggcgaggtcg acgagtcgac ggccaaggac gagtcggctg gcctcgacgc gagcgcttca
      661 gcctgggacc tctggtgccg cggcggcgac ggcgccctgc ccggcaagaa cggcgtggtc
      721 gccctctgtt cgtcgcctca tggcgccatg ctcgacgcgg tgccctacag cgagcgtttc
      781 gccgattccg ataccgccta cggcggcttc gggtctgcgg ccctgcgcga ccgggtcgcg
      841 gcggtcgtcg cggcaggagc ctgggtcggt tccgatccgc ccaggccgga ggactgcgcc
      901 aggtcggcgg gcacgacgag cacccgcagc gtctgccgct catcgtcgtc gggcgacagc
      961 gactcgggcg ccgactggca cgtcgttccg accaagggcg cttcgctcgg ctccgccaat
     1021 gccgacgagg tctacgcgcc ttgagccggg gcacggccta gaccgacggg actcgcgccg
     1081 gataagatgc ggcatggaga aacgatcgta tcgggagctt ggcgtcgccc tcgtgggcct
     1141 gccggcctcc ggcaagacca gttccggtgt cctcctggcc gacttgctcg gcatgccctt
     1201 cgtcgacctg gacgagcgcg tcgaacggga agccgggcgg tcgatcccgg ccctgttcga
     1261 ggacgaggga gaggacggct tccggcggcg ggagcgcgtg gccctcgagc acgttgccgc
     1321 tggcgtcgcc gctggcgagc ccgtggtgct ggccaccggc ggcggcaccg tcgagctacc
     1381 cgagaaccgt tccgtccttc gtgagcgctt cgtcaccgtc tggatcaagg tggatccggc
     1441 ggcggccgct gcccgaagtc tcggttcagg ccggccgctc ctgtccggcg atgtcgaggc
     1501 tgggctccgt cgtctctatg agcgcaggag ggcctggtac gatgaatgcg ccgcgatagc
     1561 cgtccagtcg gacggcatgc gcccggccat gctggcggag gccatccgtg aagcgctccg
     1621 ttagcccgtc catggtggcc gggcgcgtcg tcgccccgcc gtccaagagc gccatgcagc
     1681 gcgccgtagc ctgcgccgcc atggccgacg gggtctcgac cataacgaac ccgagcctct
     1741 gcgccgacgc gatcgccgcc tgccgcgtag ccgaggccct cggcgcctcg gtcacgcgct
     1801 acgacgaccg ggtcgtcgtg cggggcggcc tttcggccgc ccccggaatc gtcgtcgcct
     1861 cctgcggcga gtcgggcctg tgcctgcgca tgttctcggc tatcgcggcc ctgctgccgg
     1921 tcgacgtgga gctgcgggcc gagggctccc tggcctcgcg ctcggttgcg atggtcgccg
     1981 acgccctcga ggccctcggc gtgcgctgct cgacgagcgg cgggctgccg ccggtcaggc
     2041 tgcgcgggcc gctccggccg ggccgcgtac gggtcgacgg ctcggcgagc tcgcagttcg
     2101 tcaccggact cgtgatggcc ctggccatgg ccggcggtga gtcgacggtg tcggtcgagc
     2161 ggctcgcgag cggaggctac gtcgagctga ccttcgaggt gatgcgcgcc ttcggcgccg
     2221 aggccgagcg cgacgggacc gagatacgga tagccggaac gccgtaccgg gcggccgagt
     2281 acgaggtcga gggggactgg agcggcgctg ccttcatgct ggtcgccgga gccgtcgccg
     2341 gccgcggcga cctcgaggtg gtcgggctga ggcatggctc gagccagcct gac
//
LOCUS       DAOPHV010000433         2392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_62548_length_2392_cov_3.038511, whole genome shotgun sequence.
ACCESSION   DAOPHV010000433 DAOPHV010000000
VERSION     DAOPHV010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62548_length_2392_cov_3.038511"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLQ29_10455"
     CDS             <1..480
                     /locus_tag="PLQ29_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPG87108.1"
                     /translation="AQPTESGGRLVVVGSLAGSIAVPYQAYYAASKAALAAFVEALRL
                     ELVGLGAQATLVEPGDFRTGFTESRRTFGLADGSPYADNGRRAIAVMADSERHGADPV
                     LFARLVTALFEKKRLAPRYRLGPRSQRAAMRLKDLLPRRIGEALLGAYFRIYAGKGA"
     gene            483..1517
                     /locus_tag="PLQ29_10460"
     CDS             483..1517
                     /locus_tag="PLQ29_10460"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HPG87109.1"
                     /translation="MSSVKSMPFGTLPDGEQPRLFILDNGVMRVGLTEYGAALTSAIV
                     PTGKGGSLDLLLGPSSLAGYSSHQPYFGATVGRFANRIGGARFELNSRDYALTANDGK
                     NSLHGGLKGFDKRCWRAEPGTERDEAAVKMALVSPDGDQGYPGRLEASVLFTLGADCS
                     LVMRFEARSDEPTPVSLTNHAYFNLRGEGNGSVLGHELELRSSTYLPVSDELLPLPGA
                     PAPVAGGPFDFTVSKPIGRDIGAAGGYDHCFAIDGWNGTTRLAAVAVEPKSGRRLELR
                     TDMPGVQFYSGNFLSGVAGKRGSVYERHAGFCLEAEYYPDSPNRPDYPDCVLRPGRTY
                     AHTIEYRFGF"
     gene            complement(1567..>2392)
                     /locus_tag="PLQ29_10465"
     CDS             complement(1567..>2392)
                     /locus_tag="PLQ29_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87110.1"
                     /translation="MTAADAVALVRDGDAIVVSIDSDGDSGRLVVYRGTSPIRSSSDL
                     LDAALAAIVEQASGPYRDYPVPGVDYYYAIIPEKDLVAGIVELKAGANATASPVSLRA
                     GAFRVGLPSSGAASRSMPLPYLVLTRGFEDAKPVSAGDPTPRSRALKAETEKAIAALV
                     STSGSATRAERPPITIFPEDLQSGGGGEEYALRSIVAGYFSKGSYSEAVRQFTLYLSL
                     PRSEKNAAKARFYRGQAEALVGSYREAFFDLLQSQASYHIESSAWIDYILEELRRR"
BASE COUNT          380 a          796 c          827 g          389 t
ORIGIN      
        1 gcccagccta cggagagcgg cggccgccta gtcgtcgtag gctcgctcgc cggctcgata
       61 gcggtgccgt atcaggcgta ttacgccgcc agcaaggcag cgctcgcggc cttcgtcgaa
      121 gcgctgcgcc tcgagctggt cggcctgggg gcgcaggcca ctctcgtcga gccgggcgac
      181 ttcaggaccg gcttcaccga gtcccgccgt accttcggcc tggccgacgg cagcccctac
      241 gccgacaacg gccgcagggc catcgcggtg atggccgact cggagcgtca cggcgccgac
      301 cccgtgctgt tcgcgcggct cgtcacggcg ctcttcgaga agaagcgcct ggccccgcgt
      361 tatcggctcg gcccgcggtc gcagcgcgcc gcgatgcggc tcaaagacct cctgcctaga
      421 cggatcggcg aagccctgct cggggcctac ttcaggatat acgctggaaa gggagcctaa
      481 ccatgtcaag cgtcaagtcc atgcccttcg gcacgctgcc ggacggggaa cagccgcggc
      541 tgttcatact cgataacggc gtcatgcgcg tcggccttac ggaatacggc gcggccctga
      601 cctcggcgat cgtgccgacg ggcaagggcg gtagcctgga tctgctgctc ggcccgtcgt
      661 cgctggccgg ttactcgtcc caccagccgt acttcggcgc cacggtcggc cgtttcgcca
      721 acaggatagg cggcgcccgc ttcgagctga acagtcgtga ttacgctttg acggctaacg
      781 acggcaagaa ttccctccat ggcggcctca agggtttcga taagcgctgc tggagggccg
      841 agccgggtac ggagcgtgac gaggcggccg tcaagatggc gctggtaagc ccggacggcg
      901 accagggcta tcccgggcgg ctcgaggcgt cggtcctgtt cacgctcggg gcggactgct
      961 cgctcgtcat gcgcttcgag gccaggagcg atgagccgac gccggtcagc ctgaccaacc
     1021 acgcctactt caacctgcgc ggtgagggga acggctcggt gctcggccac gagctcgagc
     1081 tccgttcgtc gacctacctg ccggtcagcg acgagctact acccctgccc ggcgcgcccg
     1141 cgccggtcgc cggcggcccc ttcgacttca ccgtgtcgaa gcccataggc cgggacatcg
     1201 gcgccgcggg cgggtacgac cattgcttcg ctatcgatgg ctggaacggt acgacgcgcc
     1261 tggcagccgt ggccgtcgag cctaagagcg gacgaaggct cgagctacgc accgatatgc
     1321 caggcgtgca gttctactca gggaatttcc tgtcgggggt cgcgggcaag cgcggttccg
     1381 tttatgagcg ccacgccggc ttctgcctcg aggcagaata ctatccagac agcccgaacc
     1441 gcccggacta tcccgattgc gtactccggc cgggccggac ctacgcgcat acgatagagt
     1501 atcgcttcgg tttctgagcc gcctgtcgcc ggcgacgacg gagcctagcg ccggcgacga
     1561 cggagcctag cgccggcgaa gttcctcgag gatgtagtcg atccaggccg acgactcgat
     1621 atggtagctt gcctgcgact gcaggaggtc gaagaacgcc tcccggtacg acccgacgag
     1681 cgcctcggcc tgtccgcggt agaagcgggc cttggctgcg ttcttctctg agcgcggcag
     1741 cgacaggtac agcgtgaact gtctgacggc ctccgagtag gagcctttgg agaagtagcc
     1801 ggcaacgatc gaccgcaggg cgtactcctc gccgccgccg cccgactgca gatcctcggg
     1861 gaagatcgtt atcggtgggc gctctgcccg ggtcgctgac ccggacgtcg agacgagcgc
     1921 ggcgatggcc ttctcggtct cagccttgag ggcgcggctc cggggagtcg ggtctccggc
     1981 cgagaccggt ttggcgtcct cgaagcctct ggtcagcacg aggtagggta gcggcatgct
     2041 cctggaggcg gcgcccgacg acggcaaccc gacgcggaac gctccggccc tgagcgatac
     2101 gggcgaggcc gtcgcgttgg cgccggcctt gagctcgacg atgccggcga ccaggtcttt
     2161 ctccggtatg atagcgtagt agtaatcgac gcccgggacg ggatagtcgc ggtaggggcc
     2221 ggaggcttgt tcgacgatgg cggcgagcgc cgcgtcgagc aggtccgacg acgagcgtat
     2281 tggcgaggtg ccgcggtaga cgacgagcct gcccgaatcc ccgtcggagt ctatggatac
     2341 gacgatggcg tcgccatcgc gaaccagcgc gacggcatcg gccgccgtca tg
//
LOCUS       DAOPHV010000434         2382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_62907_length_2382_cov_4.204125, whole genome shotgun sequence.
ACCESSION   DAOPHV010000434 DAOPHV010000000
VERSION     DAOPHV010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62907_length_2382_cov_4.204125"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLQ29_10470"
     CDS             <1..357
                     /locus_tag="PLQ29_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87111.1"
                     /translation="ATVQEHQERAEHRVGHGFLPAACEEVPLLRAFCLSALTNSMFGP
                     LLMFLHRSTDNLIARASGYRGIDKSLTTLAWFWLPAHTVTFALPLPFQMGLAALWSVA
                     LGIIMGFTKRSAKEAA"
     gene            592..1068
                     /locus_tag="PLQ29_10475"
     CDS             592..1068
                     /locus_tag="PLQ29_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPG87112.1"
                     /translation="MNSRNTAQAVASLAFVLAVAVGGFFYPLAGFAVALVMAVAVAMT
                     LFRPRSFCAKACPRGRALGFALKPFSRGRPLPAGSVTPGAKRALCGFMMFCVIGNVAR
                     LAGSGAALGRFFWGLCVISLAAGVVAGLLYRPRAWCAVCPMGTLQDTIGQAIGKRR"
     gene            1188..1997
                     /locus_tag="PLQ29_10480"
     CDS             1188..1997
                     /locus_tag="PLQ29_10480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG87113.1"
                     /translation="MEIVDLSDAYVDTYCRCLEDWSDEMAEAGDRKRLWYERAKGRGL
                     RVKLATDDAGRAVGMIQYAPIEASPALGEGLYYVYCVWVHGYTEGVGNFQGRGMGSRL
                     LAAAEEDAKALGARGLVAWGLTLPFFMRSKWFKRRGYVRADSDGALELVWKPFVDGAE
                     PPRLPRRKKKPDDAPDEVVVTCIESGWCPAQNLACERARRAVSETGGDACLVELDGYD
                     RATMLEWGAGDALFIDDAQIVTGPPPSYESIRKKLERRIKKKKPKAARTGS"
     gene            complement(2046..>2382)
                     /locus_tag="PLQ29_10485"
     CDS             complement(2046..>2382)
                     /locus_tag="PLQ29_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87114.1"
                     /translation="LGAACLASCPGATDWYPSGAVAVAGSYEYDDAGVRYLAATVTVE
                     NTGSSTISLSTFTVTAKTASRTYWKTVTSDTRVLPGASIMVGAALAYADALETLVEGG
                     LTVGDAFFE"
BASE COUNT          351 a          860 c          799 g          372 t
ORIGIN      
        1 gctacggtgc aggaacatca ggagcgggcc gaacatcgag ttggtcacgg cttcctgccc
       61 gccgcctgcg aggaggtgcc cctccttcgc gccttctgcc tgtcggccct gaccaactcg
      121 atgttcggcc cgctcctgat gttcctgcac cgtagcaccg acaaccttat agcgcgcgcc
      181 agcggctacc gcggcatcga caagagcctg accacgctcg cctggttctg gctgcccgcc
      241 cacaccgtca ccttcgccct gcccctgccc ttccagatgg gcctcgcggc gctctggtcc
      301 gtggccctcg gcatcatcat gggcttcacc aagcgatcgg ccaaggaagc ggcctaaggc
      361 cgacgataga ccggcccgac agcggcccgc gtccgcgggt ccgcccggcc ggccctccgg
      421 cgtcccgggc ccccgcgcgg ccgcgctccg cctctacgca ctcgtcggcc tcctcacgcc
      481 gcgccggccg gcccgtccgg cttcgccctc gtcgcggccg ccggcgccgg cgacctgacg
      541 ctctgctccc gccagcttga tattatattg tatttttact atatagcttg catgaactca
      601 agaaacacgg cccaggccgt cgcgtcgctc gccttcgtgc tcgcggtggc cgtcgggggc
      661 ttcttctatc cgctcgcagg cttcgcggtc gccctcgtca tggccgtcgc cgtggccatg
      721 acgctgttca ggccccgctc gttctgcgcc aaggcctgcc cgcgcggccg ggcgctcggc
      781 ttcgcgctca agccgttcag ccgcggcaga ccgctgcctg ccggctcggt aacgccgggc
      841 gccaagaggg cgctctgcgg cttcatgatg ttctgcgtca taggcaacgt cgcccggctc
      901 gcgggcagcg gcgccgcgct cggccgcttc ttctggggcc tctgcgtcat atcgctggcg
      961 gcgggcgtcg tcgcggggct cctctaccga ccccgggcct ggtgcgccgt ctgcccgatg
     1021 ggcacgctcc aggacaccat tggccaggcg atcggcaagc gacgctagac gagggagtcg
     1081 cgctttgacc gcgctccgcc gttgcgctag gcttctatca gtcggctgaa ccgggcgaga
     1141 agcccgagcg tcccgcgccg ccggggcccg ccggcgggag cgtggccatg gaaatcgtcg
     1201 atctatcgga cgcgtacgtc gatacgtact gcaggtgcct cgaggactgg tcggacgaga
     1261 tggccgaggc cggcgaccgg aagcggctct ggtacgagcg agccaagggc cgcgggctcc
     1321 gcgtcaagct ggcgactgac gacgcggggc gcgccgtcgg catgatccag tacgcgccga
     1381 tcgaggcgtc gcccgccctg ggcgaagggc tgtactacgt ctactgcgtc tgggtgcacg
     1441 gctacacaga aggcgtcggc aatttccagg gacgcggcat ggggagccgg ctgctcgcgg
     1501 ccgccgagga ggacgcgaag gccctcggcg cgcggggcct cgtcgcctgg ggcctgacgc
     1561 tgccgttctt catgcgatcc aagtggttca agcggcgcgg ctacgtccgc gccgacagcg
     1621 acggcgcgct cgagctcgtc tggaagccct tcgtcgacgg cgccgagccg ccccggctcc
     1681 cgaggcggaa gaagaaaccc gacgacgcgc cggacgaggt cgtcgtcacc tgcatcgaga
     1741 gcggctggtg cccggcccag aacctcgcct gcgagcgcgc caggcgcgcc gtctcggaaa
     1801 ccggcggcga cgcctgcctc gtcgagctgg acggctacga ccgcgccacg atgctcgagt
     1861 ggggcgccgg cgacgccctg ttcatagacg acgcccagat cgtcactggg ccgcctccga
     1921 gctacgagtc gatcaggaag aagctcgagc ggcgtatcaa aaagaagaaa ccgaaggctg
     1981 cgcggaccgg ctcctgacag cgtctccgcc ctcggctgaa aagaacggct gaagagcgga
     2041 gtcgcctact cgaagaacgc gtcgcccaca gtcagcccgc cctcgaccag ggtctccaag
     2101 gcatcggcgt aggccagcgc ggcgcccacc atgatcgagg cgccgggtag cacgcgcgta
     2161 tcgctcgtca ccgtcttcca gtaggtccgc gaggcggtct tagccgttac ggtaaaggtt
     2221 gatagtgaaa tagtcgagct gcccgtgttc tcgacggtca cggtcgcggc gaggtaccga
     2281 actccggcat cgtcgtactc gtaggaaccg gccacggcca ccgctccgct cgggtaccag
     2341 tcggtcgcgc cagggcacga ggccaggcag gcggcgccga ga
//
LOCUS       DAOPHV010000435         2380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_62989_length_2380_cov_3.971183, whole genome shotgun sequence.
ACCESSION   DAOPHV010000435 DAOPHV010000000
VERSION     DAOPHV010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62989_length_2380_cov_3.971183"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLQ29_10490"
     CDS             <1..489
                     /locus_tag="PLQ29_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPG87115.1"
                     /translation="IRGVHQEHGFRAGTYNNQGILGFGLAAELAKTELEGYVERVGGL
                     RRRLRDGILAAVPKCRVNGHPERVLPNTLNVSFPGAEGEAILLSLDLEGIRVSTGSAC
                     ASGSLDPSHVLMATGVGPELAHGSIRFSLGRDSTEEDVDYVVSRIGPIIARLRSMSTV
                     SY"
     gene            527..1132
                     /locus_tag="PLQ29_10495"
     CDS             527..1132
                     /locus_tag="PLQ29_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPG87116.1"
                     /translation="MSDAFAWLYSDVVKEHFTNPKNVFNPDEGFSHNAEGMTGNIKCG
                     DQMLFMLRVEGDVIQDVRWKTYGCASAIASTSMLSEVVKGMAIKQAYNIKPNDIVAKL
                     GGLPDNKIHCSVLGDKALRAAIDDYLSKNGRSGEFRNEGARIICTCLNISDRDIEDAV
                     KGGARDWNGLQERTKIGTVCGGCKEAAEELLHEYVHIFGDA"
     gene            1227..>2380
                     /locus_tag="PLQ29_10500"
     CDS             1227..>2380
                     /locus_tag="PLQ29_10500"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HPG87117.1"
                     /translation="MKRCIKRTIAIAVVVVSCPIALAAAGGVTVPEAFSELRYSDGDD
                     PARASPSYDDSSWAMLVAPAAVDLRGGDGYFWIRARATVPSSLAAGPIWFESGKMDCA
                     FDLYAGGVYIGSRGGLPPDYYARPQTNTALLIPSSAIEGGTVSLALRCYYSGTRARIP
                     GFALADSSRADSLQHLASFFVSRINIILAVLCAFMGVYFAAGFVSKRDDTASLFYALS
                     LFFVAFYFFDMGTERLMLGGLLQRAVARSCLTASLCFSLLFFIRFFDTRGFKAAKVFA
                     ALDITAFTAAFIVFSNDDGVVDLLFNLSLAPVFAIIVIALAILVKATRKGQRDALPIL
                     IGILVGVGFAVHDIVFQVSGRQPFAWLQGFTFFFLNLSIFIATSLRSSTLR"
BASE COUNT          402 a          793 c          718 g          467 t
ORIGIN      
        1 attaggggcg tgcaccagga gcatggcttc cgggccggca cctataacaa ccagggcatc
       61 ctgggcttcg gcctcgccgc ggagctggct aagacggagc tcgagggcta cgtggagcgc
      121 gtcggcggtc tcaggcggag gctccgagac ggcatcctgg ccgccgtgcc caagtgccgc
      181 gtcaacggcc accccgagcg cgtactgccc aacacgctca acgtatcctt ccccggcgcc
      241 gagggcgagg ctatcctcct gtccctcgac ctcgagggca tccgggtgtc caccggctcg
      301 gcctgcgctt ctggcagcct ggacccgagc catgtgctga tggccaccgg cgtcgggccg
      361 gagctggccc acggctccat acgcttcagc ctaggccgtg actcgaccga ggaggacgtc
      421 gactacgtag tatctaggat cggcccgatc atcgcgcgcc tacgctcgat gtcgaccgtt
      481 tcatattaac gcgcctagcg cgcggctatt tgcggaggat ggtatcgtga gcgacgcgtt
      541 cgcctggctg tattcggatg tcgtgaagga gcacttcacg aaccccaaga atgtattcaa
      601 ccccgacgag ggtttctctc ataacgccga gggcatgacc ggcaacatca agtgcggtga
      661 ccagatgctg ttcatgctcc gcgtcgaggg cgacgtgatc caggatgtac ggtggaagac
      721 ctatggctgc gccagtgcca tcgccagcac ctcgatgctg tccgaggtgg tcaagggcat
      781 ggccataaag caggcctaca acatcaagcc gaatgacatc gtggccaagc tcggcggcct
      841 gcccgacaac aagattcact gctccgtgct cggcgacaaa gccctgcgcg cggccatcga
      901 cgactacctc tccaagaacg gccgctcagg cgagttccgc aacgagggcg ccaggatcat
      961 ctgcacctgc ctgaacatta gcgaccgcga catcgaggac gcggtcaagg ggggcgctcg
     1021 agactggaac ggcctgcagg aacgaaccaa gataggcacc gtctgcggcg gctgcaagga
     1081 ggccgcggag gagctcctcc acgagtacgt gcatatcttc ggggacgcgt agggagcgat
     1141 ccgccgggcc cttccggcat cggcgggctt gtccgcgcgc ctgactggag gctatactac
     1201 gaccagtctg acgctcggag ccctgcatga aacgttgcat caagcggacc atagcgatag
     1261 ccgtcgtcgt cgtatcgtgt ccgatcgccc tagccgcggc cggcggcgtt accgttccag
     1321 aagccttttc ggagctacgc tacagcgacg gcgacgaccc agccagggcc tccccgagct
     1381 acgacgactc gtcatgggcg atgctcgtcg ccccggccgc cgtcgatctc cggggcggag
     1441 acggctattt ctggatccgg gcgagggcta ccgtgccgtc gtccctcgcg gcagggccga
     1501 tctggttcga gagcggcaag atggactgcg ccttcgacct gtacgccggg ggcgtctaca
     1561 tcggctcccg gggcgggttg ccgcctgact actacgctag gccgcagacg aacacggcgc
     1621 tgctcatccc gtcgtcggcc atcgaaggcg gcacggtgtc cttggccctg cgctgctact
     1681 actcgggtac ccgagccagg attccgggct tcgcgctggc ggactcctcg agagccgact
     1741 cgctccagca cctcgcgtcc ttcttcgtct ccaggatcaa cataatactc gccgtgctat
     1801 gcgccttcat gggcgtctac ttcgcggccg gtttcgtctc caaacgcgac gatacggcca
     1861 gtctcttcta cgcattgtcc ctattcttcg tggctttcta ctttttcgat atgggcaccg
     1921 agcgcctcat gctaggtggc cttttgcagc gtgccgtcgc taggtcgtgc cttacggcgt
     1981 cgctgtgctt ctcgctcctg ttcttcatac ggttcttcga tacccggggc ttcaaggccg
     2041 cgaaggtctt cgccgccctc gacatcacgg cgttcaccgc cgctttcatc gtcttctcga
     2101 acgacgatgg cgtggtcgac ctattgttca acctgagcct ggcgccggta ttcgctatca
     2161 tagtcatcgc cctggccata ctcgtcaagg cgaccaggaa gggtcagcgc gacgcgttgc
     2221 ccatcctgat aggtatactc gtcggagtcg gcttcgccgt ccacgacatc gtgttccagg
     2281 tgtccggcag gcagcccttc gcctggctcc agggcttcac cttcttcttc ctcaaccttt
     2341 ccattttcat agccacctcg ctgcgatcct cgacgctacg
//
LOCUS       DAOPHV010000436         2372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_63288_length_2372_cov_4.186016, whole genome shotgun sequence.
ACCESSION   DAOPHV010000436 DAOPHV010000000
VERSION     DAOPHV010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63288_length_2372_cov_4.186016"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..548
                     /locus_tag="PLQ29_10505"
     CDS             15..548
                     /locus_tag="PLQ29_10505"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HPG87118.1"
                     /translation="MSAGFNLDDAIRKVPDFPKPGILFYDITSVLAEPDAFRYCVDAM
                     VKLYKGADLDAVAAIESRGFLFAAPLADRLGIPLILVRKKGKLPGKTIAKSYCLEYGT
                     AEIEIHEADIPKGGKVLLIDDLIATGGTLRAAADILEEGGAIPAGVFGVIGLPFLHYE
                     KALEGLEIRTLIEYFGE"
     gene            561..2228
                     /gene="gpmI"
                     /locus_tag="PLQ29_10510"
     CDS             561..2228
                     /gene="gpmI"
                     /locus_tag="PLQ29_10510"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPG87119.1"
                     /translation="MSISLVKNNAWHSRRGPVVLVVMDGVGYGKYKEGDAVAAADMRH
                     FRAMEASCPSARLKAHGTAVGLPSDDDMGNSEVGHNAIGCGRVFAQGAKLVSASIATG
                     AMFGGETWKRLVSNVRAADGRTLHFLGLFSDGNVHSHIDHLKAMLERARADGVRRVRV
                     HVLLDGRDVGEQSALEYLEPFEAFLAGLSGGGFDYRVASGGGRQYITMDRYGADWSMV
                     ERGWKAHVLGQGARFKSAKEAVLHYRAEKPSLIDQDMHEFVIEEVGEPIGAVQDGDSF
                     VYFNFRGDRALEITAAFEDDDFPHFDRIRRPKIAYAGMMEYDGDLHVPSAYLVSPPSI
                     DRTMGEYLAGSGVRMLAISETQKYGHVTYFFNGNRQGKFDEALEDYVEVKSDLVPFEQ
                     RPWMKAAEITDRVIAAIESGQYRFIRLNYPNGDMVGHTGVYQAVVCGLEAMDLQLGRL
                     RAAVEKAGGVLVLTADHGNSDDMYEHEKKTGAVTVKADGEPRAKTSHSLNPVPCVVYD
                     PEYAGEYEKELRAGLGISSLAATCIELLGFAPPKDYDPSVLEPAKAR"
BASE COUNT          434 a          816 c          747 g          375 t
ORIGIN      
        1 aacgcgaggt gcttatgtcc gcaggtttca acctagacga cgccatcagg aaggtgccgg
       61 attttcccaa gcccggcatc ctgttctacg atatcacgag cgtgctggct gagccggacg
      121 cgttccgcta ctgcgtcgac gccatggtca agctctataa gggcgccgac ctggacgccg
      181 tagccgccat cgagtcgcgc ggctttctct tcgccgcgcc gctcgccgac aggctcggca
      241 tcccgctcat cctggtacgc aaaaagggca agctacccgg caagaccatc gccaagagct
      301 actgcctcga atatggcacc gccgagatag agatccacga ggccgacatc cccaagggcg
      361 gcaaggtcct gcttatcgac gacctgatcg ccaccggcgg cacgctccgc gcggccgccg
      421 acatcctgga ggagggcggc gccataccgg ccggcgtctt cggcgtcata ggcttgccgt
      481 tcctgcacta tgagaaggcc ctcgagggtc tcgagatccg gacgctcatc gagtatttcg
      541 gagaataagg agccacgaac atgtccatct ccctcgttaa gaataacgcc tggcactcca
      601 ggcgaggccc cgtcgtgctc gtcgtgatgg acggcgtcgg ctacggcaag tataaagagg
      661 gcgacgccgt cgccgcggcc gacatgcgcc atttcagggc gatggaagcc tcctgcccct
      721 ccgcccgcct caaggcccac ggtacggcgg tcggactgcc gtccgacgac gacatgggca
      781 atagcgaagt cggccataac gccatcggct gcggccgcgt gttcgcccag ggcgccaagc
      841 tcgtctcggc ctcgatcgcg accggcgcca tgttcggcgg ggagacctgg aagcgcctcg
      901 tatcgaacgt ccgggccgcc gacggccgta cgctccattt cctcggcctc ttctccgacg
      961 gcaatgtgca ctcgcacatc gaccacctga aggcgatgct agagcgcgcc agggcggacg
     1021 gcgtcaggcg cgtccgcgtg cacgtcctcc tcgacggtcg cgacgtcggc gagcagagcg
     1081 cgctcgagta cctcgagccc ttcgaggcct tcctcgcggg gctgtcggga ggcggcttcg
     1141 actaccgcgt cgcctcgggc ggcggcaggc agtacatcac gatggaccgt tacggcgccg
     1201 actggtccat ggtcgagcgc ggctggaagg cccacgtcct cggccaggga gcccgcttca
     1261 agagcgccaa ggaagccgtc ctccattatc gcgccgagaa gccgtcgctg atcgaccagg
     1321 acatgcatga gttcgtcatc gaggaggtcg gcgagccgat cggtgccgtt caggacggcg
     1381 actccttcgt ctacttcaac ttccgcggcg accgcgcgct cgagatcacc gcggccttcg
     1441 aggacgacga ctttccccac ttcgaccgaa tcagacgccc caagattgcc tacgcgggca
     1501 tgatggagta cgatggcgac ctgcacgtgc cttcggccta cctcgtcagc cccccgtcca
     1561 tcgaccgaac gatgggcgag tacctcgccg gatccggtgt caggatgctc gcgatttccg
     1621 agacccagaa atacggccac gttacctact tcttcaacgg caaccgccag ggcaagttcg
     1681 acgaggcgct cgaggactac gtcgaggtca agagcgacct cgtacccttc gagcagcgtc
     1741 cctggatgaa ggctgccgag atcaccgacc gcgtgatcgc cgcgatcgag agcggccagt
     1801 accgtttcat ccgcctcaac tatcccaacg gcgacatggt cggccacacc ggcgtctacc
     1861 aagccgtcgt ctgcgggctc gaggcgatgg acctgcagct cggccggctc agggccgccg
     1921 tcgagaaagc gggcggcgtg ctcgtcctga ccgccgacca cggcaactcc gacgacatgt
     1981 acgaacacga gaagaagacc ggagccgtca ccgtcaaggc cgacggcgag ccaagggcca
     2041 agaccagcca ctcgctcaat cccgtgccct gcgtcgtcta cgaccccgag tacgcgggcg
     2101 agtacgagaa ggagctgcga gccgggctcg gcatcagctc gctcgcggcc acctgcatcg
     2161 agctgctcgg cttcgcgccg ccaaaggact acgacccgtc cgtgctcgag ccggccaagg
     2221 cccgatgacc gaggtagagc tcaaggcgcg ggtcagggat ccgtccgcgg tggaggcgag
     2281 ggtcgcgtcg ttcgcggcct tcgtccgttc cttcgacaag gcggacgagt actggcacgg
     2341 gccggactgg cgcttcacgc gcggaaccaa gg
//
LOCUS       DAOPHV010000437         2370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_63376_length_2370_cov_3.294168, whole genome shotgun sequence.
ACCESSION   DAOPHV010000437 DAOPHV010000000
VERSION     DAOPHV010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63376_length_2370_cov_3.294168"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PLQ29_10515"
     CDS             complement(<1..1010)
                     /locus_tag="PLQ29_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG87120.1"
                     /translation="MSRYDKYSDSALDVLVKTQDIALRGPIGETGHRDLAEALMLFDL
                     PDIIAIAKSNNAVVDVELIKSLNERLESSAPREVEGPIKISGEFRSILERAEALAGDA
                     KVEPSHLIRAAWPTIKGELAPFFRLEAAPALAIPADEIALPEIGPRAESAEAIAALLR
                     FGDELTGPDADFPVFGREAELDALSSVLMRYWKPNPLIVGESGVGKTALVQGLAMRIK
                     SGDVPARLRNARVFEVRVSELLAGTAMHGALEENVNQLVEAAESLQDVYLFIDEIHQV
                     VPAFANNPISETLKPALSSGRIRCIGATTSADYARYLEKDTALLRRFQTVLVKEPDET
                     TIG"
     gene            complement(1013..1576)
                     /locus_tag="PLQ29_10520"
     CDS             complement(1013..1576)
                     /locus_tag="PLQ29_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87121.1"
                     /translation="MPTKLSTIESYLIAERLKYSVHDDYIRTSFATDLYRDQDGDSSV
                     FIIARVEEDGEYFKLMAPNLYRYPPDGPNAGWVYRTLLGICWRSKLIKYEYDERDGEI
                     RAIVEFPIEDAELGPRQFMRCLNGLVQIIDEYHPAVAAAMAGGPASLDDAEETADNVR
                     RAERILRAAGVDRAKLSLAGSPVRLEE"
     gene            1768..>2370
                     /locus_tag="PLQ29_10525"
     CDS             1768..>2370
                     /locus_tag="PLQ29_10525"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPG87122.1"
                     /translation="MDRGYCVIPAAGRSSRMGDWKPLLPWGHSTICGAVADAALAAGL
                     RPVVVAGYRADELAAAFSGRPEVAVVVNRDWERGMLGSVRVGAALPLSRGSGFFVAPA
                     DMPRLPARAFERALAELDRLEQAGEATRTLFASRAGRLGHPVWIPASVAVGLGALDPG
                     LRLRDHVAANPGLRDVMLAGHGIICWGDTAEACYRNTISLI"
BASE COUNT          327 a          782 c          836 g          425 t
ORIGIN      
        1 cctatcgtcg tctcgtcggg ctccttgacc agcacggtct ggaagcgcct gagcagggcg
       61 gtgtcctttt caaggtagcg cgcgtagtcg gcgctcgtcg tcgccccgat acagcgtatg
      121 cgcccggagg acagggccgg cttgagggtc tccgagatcg ggttgttggc gaaggcgggc
      181 acgacctggt ggatctcgtc tatgaacagg tagacgtcct ggaggctctc ggccgcctcg
      241 acgagctggt tgacgttctc ctccagggcg ccgtgcatcg cggtgccggc gaggagctcg
      301 gagacgcgca cctcgaagac gcgggcgttc ctgaggcggg ccggcacgtc gcccgacttg
      361 atccgcatcg cgaggccctg gacgagggcg gtcttgccga cgcccgactc gccgacgatc
      421 agcgggttgg gcttccagta tcgcatgagc accgagctga gcgcgtcgag ctcggcctcg
      481 cggccgaaga ccgggaagtc ggcgtccggc ccggtcagtt cgtcgccgaa gcgcaggagc
      541 gcggcgatcg cctcggcgga ttcagcccgc ggtcctatct cgggcaacgc tatctcgtcg
      601 gccgggatgg ccagggcggg cgccgcctcc agcctgaaga acggcgccag ttcgcccttg
      661 atcgtcggcc aggcggcccg tatgaggtgg gacggctcga ccttcgcgtc gcccgcgagt
      721 gcctcggccc gctcgagtat cgacctgaac tcgccggaga tcttgatcgg cccctcgacc
      781 tcgcgcgggg cgctcgactc gaggcgctcg ttgagcgact tgatcagctc cacgtcgacg
      841 accgcgttgt tgctcttggc gatcgcgatg atgtccggca ggtcgaacag catcagggcc
      901 tcggccaggt cgcggtggcc ggtctccccg atggggccgc gcagggctat gtcctgggtc
      961 ttgacgagga cgtccagggc gctgtccgaa tatttatcgt acctggacat gcctactcct
     1021 cgagcctgac cggggatccg gctaggctca acttggcccg gtcgacgccg gcggccctca
     1081 ggatccgctc ggccctgcgc acgttgtcgg ccgtttcctc cgcgtcgtcg agcgacgccg
     1141 ggccgccggc catggcagcg gcgacggccg ggtggtactc gtcgatgatc tgcacgagac
     1201 cgttcaggca acgcatgaac tgcctggggc cgagctcggc gtcctcgatc gggaattcga
     1261 cgatggcccg tatctcgccg tcccgctcgt cgtactcgta cttgatcagc ttggaacgcc
     1321 agcagatacc gagcagggtc cggtacaccc agccggcgtt cggcccgtcg ggcggatagc
     1381 ggtagaggtt cggcgccatc agcttgaagt actcgccgtc ctcctcgacg cgggcgatga
     1441 tgaacacgct cgagtcgccg tcctggtcgc ggtagaggtc ggtcgcgaag ctcgtacgga
     1501 tatagtcgtc gtgcaccgag tatttgagcc gctcggcgat caggtacgat tctatggtcg
     1561 acagcttcgt tggcacgcgt tcatctcctc gggggccggt ttcgattggc ttctacgtat
     1621 agtatatcga gaagcggacc cgatttggaa ggccgagcgc ggttctaatc gaatcatccg
     1681 tcaccgcggc ttgcgttgac gggcctccta tcgcatactc gcggcatggt ttcagtcgat
     1741 gctgatgaga ggcgatgccg ggaacggatg gaccggggct actgcgtcat cccggctgcg
     1801 gggcgctcgt cgcggatggg cgactggaag cccctcttgc cctggggcca ctcgacgata
     1861 tgcggcgccg tggcggacgc ggccctggcc gcggggctgc ggccggtcgt cgtggccggc
     1921 taccgggcgg atgagctggc cgccgcgttc tcgggccggc ccgaggtcgc ggtcgtcgtc
     1981 aaccgggatt gggagcgcgg catgctcggc tccgtgcggg tcggcgcggc cctgcccctg
     2041 agccggggct caggcttctt cgtggcgccc gcggatatgc cgaggctgcc ggcccgggct
     2101 ttcgaacggg cgctcgccga gctggaccgg ctcgagcagg caggcgaggc cactcggacc
     2161 ctgttcgcct cgagagccgg gcgcctcggc cacccggtct ggatacccgc ttcggtcgcg
     2221 gtaggactcg gcgccctcga cccgggactg cgcctgcgcg atcacgtggc cgcgaatccc
     2281 gggttgcgcg acgtgatgct cgccggccac ggcatcatct gctggggcga caccgccgag
     2341 gcctgctacc gcaacaccat cagcctgatc
//
LOCUS       DAOPHV010000438         2367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_63464_length_2367_cov_4.615484, whole genome shotgun sequence.
ACCESSION   DAOPHV010000438 DAOPHV010000000
VERSION     DAOPHV010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63464_length_2367_cov_4.615484"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLQ29_10530"
     CDS             complement(<1..205)
                     /locus_tag="PLQ29_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87123.1"
                     /translation="MRSDKTVDEKSGQWGEASQGDRLALSRTLLSIMKDGVFFADRSL
                     RIRYANHAFVMSTSLDSFVDKPPT"
     gene            320..1042
                     /locus_tag="PLQ29_10535"
     CDS             320..1042
                     /locus_tag="PLQ29_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87124.1"
                     /translation="MLRLLISALICIFSLSIARPASASPASAPADWWAAAMEASRLAS
                     GYEASRTRISIEALDASGAVVSYERGETRLQRVDGREVVVVVSAEKDGKDASDDWRKR
                     YAKAAESASRRDGRADRGGPPPGFDAAPFNPAYVGALSIGEAATRGASVEVSFSIDTE
                     AGRVVGVVSFAGSGVALSSTQSWLEPPPLVSFMRSSTRYAYHRGALVVASMELEGEMS
                     ILFVKRRFRMRFEFSEWIPAGG"
     gene            complement(1039..2025)
                     /gene="holA"
                     /locus_tag="PLQ29_10540"
     CDS             complement(1039..2025)
                     /gene="holA"
                     /locus_tag="PLQ29_10540"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPG87125.1"
                     /translation="MEPVFALGGPELGRRNDFIKQLRSRCASEWNGEPEDHRLYAHET
                     PVSALLDLLLNGSLFSAGKFVQYLGADQVKAKADVQAIVEYAKRPADSTVLVLVADGI
                     GVDKAIEAAIPKDAKKVFWELSAQETGRWVRDYFSGQGLSVDDDAVEAILELVENNTE
                     ALKVECSRLALFFPRGSSVGEDDVERYIAHNRAEDAFSLFDRMATGDFEQALETLAAI
                     QAARDGSGVGLLAGLLWSFRRLSSLHAAMADGQGFEQAARSLRITRRSALASYDAARQ
                     RWPHGRCDRLIAFGVDVDARLRAMGQANERTLLELFVYACVVRRSPVPLGRL"
     gene            complement(2032..>2367)
                     /gene="lexA"
                     /locus_tag="PLQ29_10545"
     CDS             complement(2032..>2367)
                     /gene="lexA"
                     /locus_tag="PLQ29_10545"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPG87126.1"
                     /translation="GKPIFAEENFDSSIHVPVSMVGSSRCFALKVKGDSMIKAGILDG
                     DLAVIEQRQVASDGEIVVAAIEDSVTLKRFYKEKTRVRLEPENDSYAPIFTQDVRILG
                     KLRGIVRNY"
BASE COUNT          370 a          767 c          791 g          439 t
ORIGIN      
        1 gagtcggcgg cttgtcgacg aaggagtcga ggctcgtgga catgacgaac gcgtggttcg
       61 cgtaccgtat cctgaggctt ctgtcggcga agaatacgcc gtccttcatg atggatagca
      121 gggtgcgcga gagggcaagc ctgtcgccct gcgacgcctc gccccactga ccgcttttct
      181 cgtctaccgt cttgtcgcta cgcatacgcg ttgaataccg tccagttcgc cacgaccgag
      241 ttcacgcttg tatccgacac ggcaggcaag atttcactat actctagcat ggatgacccc
      301 tgtcgtggaa ggagcggcgt tgttacgttt attaatatcg gcactaatct gcattttctc
      361 tctgtcaatc gcgcgtcccg cttcggcttc cccggcgagc gccccggccg actggtgggc
      421 cgccgcgatg gaggcgagcc gcctcgcttc gggctacgag gctagtagga cgcgcatctc
      481 gatagaggcg ctcgacgcct cgggggccgt ggtctcgtac gagcgcggcg agacgaggct
      541 ccagcgggtc gacgggaggg aagtggtggt cgtcgtctcc gccgagaagg acggcaagga
      601 cgcgagcgac gactggagga agcgctacgc caaggcggcg gagagtgcct ctcgcagaga
      661 cggccgtgcc gatcggggcg ggccgccccc gggtttcgac gcggcgccgt tcaacccggc
      721 ctacgtcgga gccctgagca tcggcgaggc cgctacgcgg ggcgcttcgg tcgaggtgtc
      781 gttctcgatc gataccgagg ccggccgcgt cgtcggcgtc gtctcgttcg ccggctccgg
      841 ggtggccttg tcgtcgaccc agtcctggct cgagccgccg cccctggtat cgttcatgcg
      901 ctcgtcgacg cgctacgcct accatcgggg cgccttggtc gtcgcgtcga tggagctgga
      961 gggggagatg tccatcctgt tcgtcaaaag gcgtttcagg atgcgcttcg agttctctga
     1021 gtggattccg gccggaggtt agagccgtcc gagcggtacc ggggacctac gcaccacgca
     1081 ggcgtacacg aacagctcga ggagcgtccg ctcgttggcc tggcccatcg cccgcagcct
     1141 ggcgtccacg tccaccccga aggctatcag ccggtcgcag cggccgtgcg gccagcgctg
     1201 gcgcgccgcg tcgtagctgg ccagcgcgga tcggcgggtg atgcgtagcg agcgcgccgc
     1261 ctgctcgaag ccctgaccgt cggccatcgc ggcgtggagc gacgagaggc gtctgaacga
     1321 ccaaaggagc cccgctagca ggccgacccc cgagccgtcc cgtgcggcct ggatcgccgc
     1381 gagggtctcg agcgcctgct cgaagtcgcc cgtggccatc ctgtcgaaca ggctgaacgc
     1441 gtcctcagcc cgattatggg cgatgtagcg ctcgacatcg tcctctccga cgctcgagcc
     1501 gcgcgggaag aacagcgcca ggcgcgaaca ctcgaccttg agggcctcgg tgttgttctc
     1561 gactagctcg aggatggctt cgaccgcgtc gtcgtcgacc gacagtccct gcccggagaa
     1621 atagtccctg acccagcggc cggtctcctg agccgacagc tcccagaaca ccttcttggc
     1681 gtccttgggg atggcggcct cgatggcctt atccacgccg atgccgtcgg cgacgagcac
     1741 gagcacggtc gagtcggccg gccgtttcgc gtactcgacg atggcctgga cgtcggcctt
     1801 ggccttgacc tggtcggcgc cgaggtactg cacgaatttc cccgccgaga acagcgagcc
     1861 gttcagtagg agatcgagca atgccgacac cggggtctcg tgggcgtaga gcctatggtc
     1921 ctccggctct ccgttccatt ccgaggcgca acgcgaccgc agttgcttga tgaagtcgtt
     1981 gcggcggccc agttcggggc cgccgagggc gaatacgggt tccaccggcg ttcagtagtt
     2041 cctgactatg ccgcggagct tgccgagtat gcggacgtcc tgggtgaaga tgggcgcgta
     2101 gctgtcgttc tccggctcca gcctgaccct cgtcttttcc ttgtagaagc gtttgagcgt
     2161 aaccgagtcc tcgatggccg ctacgacaat ctcgccgtcg ctggccacct gtcgctgctc
     2221 gatcacggcc aggtcgccgt cgaggatgcc ggccttgatc atgctgtcgc ccttgacctt
     2281 aagggcgaag caacgcgacg agccgaccat cgagaccggt acgtggatgc tcgagtcgaa
     2341 attctcctcg gcgaagatcg gcttgcc
//
LOCUS       DAOPHV010000439         2357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_63822_length_2357_cov_3.354040, whole genome shotgun sequence.
ACCESSION   DAOPHV010000439 DAOPHV010000000
VERSION     DAOPHV010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63822_length_2357_cov_3.354040"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLQ29_10550"
     CDS             complement(<1..420)
                     /locus_tag="PLQ29_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPG87127.1"
                     /translation="MVRKLLCLVAFVCVCVSAYAGPPRMAIGDFTVTSDNPKLKYVGK
                     GFAEMIGAELALSKGVVLIDRAKRDQLLGELEFSLSGLSDSDSMLRAGELLAADYLLF
                     GDLVDLGDAVLVSCKMVKTETGEVAWQDKYLGPLADYD"
     gene            complement(648..2006)
                     /locus_tag="PLQ29_10555"
     CDS             complement(648..2006)
                     /locus_tag="PLQ29_10555"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPG87128.1"
                     /translation="MAEQTRDVTMEKITSLAKRRGFVFQSSEIYGGQAGAWDYGPLGI
                     ELKNRIQRFWWKEMTQLHDDIVGLDAAILMHPRTWEASGHVENFTDPLVDCKKCKQRF
                     RADLIDPGKLAAKTCPECGGELTDTRKFNLMFKTHIGPVDDGTGLIYLRPETAQGIYV
                     NYKNIVQSNRMKIPFGIAQVGKAFRNEIVTKNFIFRTCEFEQMEMQYFVKPGDDEKYF
                     DYWRGQRWAYFQKLGVRMDKLRWHQHGKDELAHYAKDAYDIEYEFPMGWKELEGVHNR
                     TDFDLKRHQEFSGKDLQYIDQDNGNERFVPYIIETSAGLTRQLLMVLCDAYEEEKVAD
                     KGNDDDWRTVMKFHPELAPITVAVMPLMKKDGLAELAQEIRSELKHDYATDYDQSGAI
                     GRRYRRQDEAGTPYCVTIDYDSKENGTVTVRDRDTMEQERVHKDELSAYIRNKIKNYV
                     RK"
BASE COUNT          384 a          708 c          788 g          477 t
ORIGIN      
        1 gtcgtagtcc gcgagcgggc cgaggtactt gtcctgccag gcgacctcgc ccgtctcggt
       61 cttgaccatc ttgcaggaga cgagaactgc atcgcctagg tcgactaagt cgccgaagag
      121 gaggtagtcg gcggccagga gctcgcctgc cctgagcatg gagtctgagt cggacaggcc
      181 cgagagcgag aactcgagct ccccgaggag ctggtcgcgc ttggctcggt cgatgagcac
      241 gacgcccttg gacaaggcca gttcggcgcc tatcatctcg gcgaagccct tacctacata
      301 cttgagcttg ggattgtccg acgtaacggt aaagtcgccg atggccatac ggggcggccc
      361 tgcgtaggcc gagacgcaga cgcagacgaa cgcgacgagg cacaggagct tgcgaaccat
      421 actagcctcc attccggagg ctaagtatat ggccgctcgg cgtctttgca atggttcgta
      481 gccttatcga ggcgtcgcga accagaagtc aggcgggccg gcgccgcgcg agggatcgga
      541 gcggaaaccc cggagcgaag aaggaaaggc gcctctcggc gcctttcctt cttcacgagg
      601 agttggagcg gagagcccga cccggcgtag ccgggtcgcg cccagcctta tttccttacg
      661 tagttcttta tcttattcct gatataggcg gacagctcat ccttgtgcac ccgctcctgc
      721 tccatggtgt cgcggtcgcg aacggtgacg gtgccgttct ccttggagtc gtagtctatg
      781 gtgacacagt acggagtgcc ggcctcgtcc tgacggcggt atcggcggcc gatggcgccc
      841 gactggtcgt agtcggtggc gtagtcgtgc ttgagctcgg agcgtatctc ctgggccagc
      901 tcggcgaggc cgtctttctt catcaggggc atgaccgcga cggtgatggg cgccagctcc
      961 gggtggaact tcatgacggt gcgccaatcg tcgtcgttgc ccttgtcggc caccttctcc
     1021 tcctcgtagg cgtcgcagag gaccatgagg agttgccggg taagaccggc cgaggtctcg
     1081 atgatgtacg gcacgaagcg ctcgttcccg ttgtcctggt cgatgtactg gaggtccttg
     1141 ccggagaatt cctggtggcg cttcaagtcg aagtcggtac ggttgtgcac gccctcgagc
     1201 tccttccagc ccatcgggaa ctcgtactcg atgtcgtagg cgtccttggc gtaatgcgcg
     1261 agctcgtcct tgccgtgctg gtgccagcgg agcttgtcca tgcgcacgcc gagcttctgg
     1321 aagtaggccc agcgctgccc gcgccagtag tcgaagtact tctcatcatc gcccggcttg
     1381 acgaagtact gcatctccat ctgctcgaac tcgcaggtgc ggaagatgaa gttcttggtg
     1441 acgatctcgt tacggaacgc cttgccgacc tgggctatgc cgaaagggat cttcatgcgg
     1501 ttggactgga cgatgttctt gtagttgacg tagatgcctt gcgccgtctc aggccgcagg
     1561 tagatgaggc cggtgccgtc gtcgactggg ccgatgtgcg tcttgaacat caggttgaac
     1621 ttgcgggtgt cggtcagttc gccaccgcat tcggggcagg tcttggccgc tagcttgccc
     1681 gggtctatga ggtcggcgcg gaagcgttgc ttgcacttct tgcagtccac cagggggtcg
     1741 gtgaagttct cgacatggcc cgaggcctcc caggtgcgcg ggtgcatgag gatggcggca
     1801 tccaggccga cgatatcgtc gtggagctgg gtcatctcct tccaccagaa gcgctggatg
     1861 cggttcttaa gctctatgcc caggggaccg tagtcccagg cgccggcctg cccgccgtag
     1921 atctccgagc tctggaagac gaagcccctg cgctttgcca gggaggtgat cttctccatc
     1981 gttacgtcgc gagtctgctc tgccatgtga tgctccttgc cccccgtggc gcgaggggac
     2041 tgccctgtac cccgctcgcc gaccggcgcc gttacggcgc gcccggcgga gggcctagag
     2101 gctggggttt ggtgtcgcgc tcgcgtctcg gcgggccgag cggctagcgc gcgatcggga
     2161 gtatagcccg gccgcccggg catgggcaag gctgagcgcc tagagggcta tgcggcgcct
     2221 gaccagctcg acgacgtgtg gaaggacggc aacgtggtga ccttctcgct gacccagacg
     2281 agggacgagt actgctacta taacggggcg accgagcccg acgccccggc cgcggacgcg
     2341 gacggctaca actccgt
//
LOCUS       DAOPHV010000440         2337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_64586_length_2337_cov_3.538124, whole genome shotgun sequence.
ACCESSION   DAOPHV010000440 DAOPHV010000000
VERSION     DAOPHV010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64586_length_2337_cov_3.538124"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..>2337)
                     /locus_tag="PLQ29_10560"
     CDS             complement(169..>2337)
                     /locus_tag="PLQ29_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPG87129.1"
                     /translation="VVRLSRLTPLRPVDAASRIIGKQGSCLAVEELWALGMWAAAYAG
                     LVRWLSGVRHEGLRAELTAAPDLSAVAAAAFRVVGQDGEIRDLPELRDIRSKIRAINL
                     DIERVTASFFQDESTRQMLQNDVPTQRDGRTVLAVKAGSRSRLKGIVHEVSSTGQTVY
                     IEPETLVQRNNELVEEEARYLRELQRVLRETTAKLYNYSGAVAAARVACAAFDGLYGK
                     ARYSHLNDGVFAEERGSGLDLVKARHPILGSKAVPIDLCMPEDARTLIITGPNTGGKT
                     VSLKTAGLLALMNQFGLAVPAGYGTALQVFDGVWADIGDEQSMDQSLSTFSGHMRSIS
                     AIVAGATSRSLVLLDELGSGTDPEEGCAIAMALLDLFIGRGALAIVTTHHGILKNFGY
                     TKEGVLNASVDFDKDTLSPTYRILMGVPGESRALDIAGKNGVSTEVVEGARRYLRDER
                     TDVSALIRGLSDKHRELDSMRDEERRRLRDAKEERRKADLKELKLRQRESELRTQGVA
                     ELKALLLDSRKTLENLVRELREGELTREKTQAVKEFILDLERKVEEADEKARSNEREL
                     KAGSRPPAEIGEGCQVLILPSRRRGKALRAVKGGKWLVETDALRLTVDEADLEPTFEL
                     ERKAPSISIEASSSRSNRAVIELDLRGYRLSEAIAALERQLDAATMEGLSGFSIIHGT
                     GEGVLQQGVRETLARYPGVADFHYARPEDGGHGKTIVSFA"
BASE COUNT          348 a          771 c          796 g          422 t
ORIGIN      
        1 cgacgacaat ctgtcgatcc tgcgtaccga gaaggtgctc aggccgggcc tcgaaccgcg
       61 gttatgggag gccgtcatcg ggcgcgtagc cgcgagggac gtacccgacg gcgagggcct
      121 cgagtgggcc gacatcgggg gcgcctggat cggttcctag ggggtcgctc aggcgaagga
      181 gactatcgtc ttgccgtggc cgccatcctc gggcctggcg taatggaagt cggccacgcc
      241 cggatagcgt gccagggttt ccctgacgcc ctgctgcagc acgccctcac cggtgccgtg
      301 gatgattgaa aagcccgaca agccctccat cgtcgcggcg tcgagctggc gctcgagcgc
      361 ggctatggcc tcggacagcc tatagccgcg cagatcgagc tcgatgacgg cgcggttcga
      421 gcggctggag ctagcctcga tggagatgct cggcgccttc cgctccaact cgaaggtcgg
      481 ctcgaggtcg gcctcgtcga cggtcagacg cagcgcgtcg gtctcgacga gccatttgcc
      541 gcccttgacc gcgcgcaagg ccttgcccct acgtcgcgaa ggcaggatca gtacctggca
      601 gccctcgccg atctcggcgg gaggcctgga gccggccttg agttcgcgct cgttcgagcg
      661 ggccttctcg tcggcttcct cgaccttgcg ctcgaggtcg aggatgaact ccttgaccgc
      721 ttgggtcttc tccctggtca attcgccctc gcgcagttcg cgcaccaggt tctcgagggt
      781 tttgcggcta tcgaggagaa gtgccttgag ctcggcgacg ccttgggtgc gtagctccga
      841 ctcgcgctgg cggagcttga gctccttgag gtctgccttt cgccgctctt ccttcgcgtc
      901 gcgcagcctt cgccgttcct cgtctcgcat cgagtcgagc tcgcggtgct tgtccgacag
      961 gccgcggatc aacgccgaga cgtcggtgcg ctcgtcgcgc aggtagcgcc tcgcgccctc
     1021 gaccacctcg gtcgagacgc cgttcttgcc ggcaatatcg agggcgcggc tctctccggg
     1081 cacgcccatc aggatacggt aggtcggcga aagggtatcc ttgtcgaaat cgaccgacgc
     1141 gttcagcacg ccctccttcg tgtagccgaa gttcttgagt atgccgtgat gggtcgtgac
     1201 tatggctaag gcccctcgtc cgatgaacag gtcgaggagg gccatcgcta tggcgcagcc
     1261 ctcctccggg tcggtacccg agcccagctc gtcgagcagc acgaggctgc ggctggttgc
     1321 gccggctacg atggccgaga tcgagcgcat atgcccggag aaggtcgaca gcgactggtc
     1381 catcgactgt tcatcaccta tatcggccca gacgccgtcg aagacctgga gcgccgttcc
     1441 gtagccggcc ggcaccgcca ggccgaactg gttcatcaag gccagcaggc cggcggtctt
     1501 gagcgacacg gtcttgccgc cggtgttcgg gccggtgatg atcagggtgc gcgcgtcctc
     1561 gggcatgcac aggtcgatcg gtacggcctt ggacccgagt atcggatgcc tggccttgac
     1621 caggtccagg cccgagccgc gctcctcggc gaacacgccg tcgttcaagt gggaatagcg
     1681 ggccttgccg tataggccgt cgaaggcggc gcaggcgacc ctggccgccg cgacggctcc
     1741 cgagtagttg tagagcttgg ccgtcgtctc ccggagcacg cgctggagtt cccgcaggta
     1801 gcgcgcctcc tcctcgacca gctcgttatt gcgctggacg agggtctccg gctcgatata
     1861 gacggtctgc ccggtggacg acacctcgtg tacgatgccc ttgagcctcg agcgggaacc
     1921 cgccttgacc gctagcacgg tccgaccgtc gcgctgggtc ggtacgtcgt tctggagcat
     1981 ctgtcgggtc gactcgtcct ggaagaagga ggccgtgacg cgctctatgt cgaggttgat
     2041 ggctcgtatc ttcgagcgga tatcgcgaag ctcgggcagg tcgcgtatct cgccgtcctg
     2101 cccgacgacc ctgaacgcgg cggccgctac cgccgacagg tcgggcgcgg ccgtgagctc
     2161 ggcgcgtaac ccttcgtgcc tgacgccgga cagccaacgc acgagcccgg cgtaggcggc
     2221 ggcccacatg ccgagcgccc agagttcctc cacggccagg cacgagccct gcttgccgat
     2281 gatgcgcgag gcggcgtcga cggggcggag gggcgtgagc ctggagagcc ggacgac
//
LOCUS       DAOPHV010000441         2336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_64616_length_2336_cov_4.268303, whole genome shotgun sequence.
ACCESSION   DAOPHV010000441 DAOPHV010000000
VERSION     DAOPHV010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64616_length_2336_cov_4.268303"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PLQ29_10565"
     CDS             <1..541
                     /locus_tag="PLQ29_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87130.1"
                     /translation="GVNLAFGSYFLYCSLAVDTLANRPVSGGTYLFWFVARFFSELFA
                     DPLGVIAVGLGMVPLVFSVLFWAIPALRSGSVARQNERIRRENLRRVVYAKAVERPSA
                     TQAPEPESLPAVARPSGPDEPRKALEALAAYEGGEPIEGGAWRLAELERKLADAEAVR
                     RAVRPEDYELGGVAFDSGS"
     gene            538..1428
                     /locus_tag="PLQ29_10570"
     CDS             538..1428
                     /locus_tag="PLQ29_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPG87131.1"
                     /translation="MSQSPSAEAAPSRATGVALAVVSAVTFSTLGLFAKLAYSQGMAP
                     AQALAWRFSLAAAILWCVLLKQGGHRRPWRQYRSAMLLGAFGFAPQAGLFFVTVRFLD
                     PGIASLLLYLYPAFVVGFWFLFFGRRPRREQLIALALSGAGCVLTLWTRGSYPLIGYL
                     LGLSVAVSYAGYLVIGERVTVGIDPTFLTANLMTVSAVVYWSVTAVSGSFLLPSTPAS
                     IAGVVGIAVVGSVIPIVTLFASIRIIGSSDASLVSTIEPLFTVSLSALLLGERLTPLQ
                     LAGGALILLGVLLLNLRKRR"
     gene            complement(1425..2246)
                     /locus_tag="PLQ29_10575"
     CDS             complement(1425..2246)
                     /locus_tag="PLQ29_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87132.1"
                     /translation="MSELLRLLPPIRRMRGNRLYAADGRRFLDLWLDDGRGILGHRDR
                     LARTAAANAADKGLLRPYPGLYDARLAKSVSSAWPGFGTVRAFESDERAVQAAGRVAA
                     RIEAQSSGSSSIQVGGQRREPYLARPFAPVPDGLDLVMPRLPCPRPFAPACLAARDGS
                     PAAQALAAEPGDLVSAQACLAAARGLASLGSAAADGYDETLWKRFDRRMAAFFEREGP
                     YLRARVPSERYEAFFRAALTGGALVSPEPGSPSVVPPEFDDGELVKLARSLESSL"
BASE COUNT          303 a          810 c          816 g          407 t
ORIGIN      
        1 cggagtcaac ttggccttcg gttcctattt cctatactgc tcgctggcgg tagacacgct
       61 agcgaaccgg ccggtctcgg gcggcactta cctcttctgg ttcgtcgcca ggtttttctc
      121 ggagctgttc gccgatccgc tcggcgtcat agccgtcggt ctcggcatgg tgccgctggt
      181 cttctcggta ctgttctggg ccataccggc cctgcgctcc ggctcggtgg ccaggcagaa
      241 cgagcgcatc aggcgcgaga acctgcgccg tgtggtctac gcgaaggcgg tggaacggcc
      301 gtcggcgacc caggcgccgg agcccgagtc actgcccgcg gtcgcgaggc cgtcgggtcc
      361 ggacgagccg cgtaaggcgc tcgaggccct ggccgcctac gagggcggcg agccgatcga
      421 aggcggcgcc tggcgcctcg cggagctgga gcgcaagctg gccgacgcgg aggccgtgcg
      481 ccgggcggtc cgtcccgagg actatgagct cggcggcgtc gccttcgact ccggatcgtg
      541 agccagtccc cgtcggccga ggccgctcca tccagggcca cgggcgtcgc gttagccgtc
      601 gtctcggcgg tcaccttctc gacgctcggt ttgttcgcca agctggccta ctctcagggc
      661 atggcgccgg cccaggctct ggcctggcgc ttcagcctgg cggccgccat cctgtggtgc
      721 gtcctcctca agcagggcgg gcatcgcagg ccgtggcggc agtaccgctc ggccatgctg
      781 ctcggcgcct tcggcttcgc gccccaggcc gggctgttct tcgtcacggt caggttcctc
      841 gacccgggga tagcgtcgct gctcctatac ctatacccgg ccttcgtagt cgggttctgg
      901 ttcctgtttt tcggccgcag gcctaggcgc gagcagctga tcgccctggc gctatccggc
      961 gccggttgcg tcctgactct atggaccagg ggctcgtatc cgctcatcgg ctacctactc
     1021 ggcttgtcgg tagccgtcag ctacgccggc tacctcgtca tcggggagcg cgtcaccgtc
     1081 ggcatcgacc cgaccttcct gacggccaac ctgatgacgg tatcggccgt cgtctactgg
     1141 agcgtcacgg ccgtctccgg ctcgttcctg ctgccttcaa cgcccgcgtc gatagccggc
     1201 gtcgtcggta tcgccgtggt cggatcggtc atacccatcg tcacgctgtt cgcgtccatc
     1261 cgcatcatcg gctcgtccga cgcgtcgctc gtgtcgacga tagagccgct gttcaccgtg
     1321 agcctatcgg cgctgctgct cggcgagcgg ctgacgcccc tccagctagc cggcggggcg
     1381 ctgatcctcc tcggcgtgct gctcctgaac ctgcgtaagc ggcgctagag cgacgactcg
     1441 aggctcctgg ccagcttgac gagttcgccg tcgtcgaact cgggcggcac gaccgagggc
     1501 gagcccggct cgggcgatac gagggcgccg cccgtgagcg cggccctgaa gaaagcctcg
     1561 tagcgttcgg acggcaccct ggctcgcagg tagggacctt cgcgctcaaa gaaggcggcc
     1621 atgcggcggt cgaagcgttt ccacagcgtc tcgtcgtagc cgtcggccgc cgccgacccg
     1681 aggctggcga gaccgcgcgc ggccgccagg caggcctggg ctgacacgag gtcgcccggc
     1741 tcggccgcga gcgcctgcgc cgccgggcta ccgtcgcggg ccgcgaggca ggccggcgcg
     1801 aagggcctcg ggcagggtag gcgcggcatg acgaggtcaa gaccgtcagg gaccggcgcg
     1861 aagggcctgg cgagatacgg ctcgcgccgc tgtcctccga cctggatgga ggacgatccg
     1921 ctcgattgag cttcgatgcg cgcggccacg cggcccgccg cctggacggc tctctcgtcg
     1981 ctctcgaagg cccgcaccgt accgaagccc ggccaggccg acgacacgga cttggcgagg
     2041 cgcgcgtcgt atagcccagg atagggccgt aggaggccct tgtcggcggc gttggcggcg
     2101 gccgtacgcg cgaggcggtc gcggtggccg aggataccgc ggccgtcgtc gagccacagg
     2161 tcgaggaagc gcctgccgtc ggcggcgtag agtcggttgc cgcgcatccg gcgtatcggc
     2221 ggcaataatc tcaagagctc gctcatcgct tctccgtggc tagtcgccca tgcgctctag
     2281 cttcttggta aacttgggaa agaactggag cgtccgcccg gtctcgaagc ggacgg
//
LOCUS       DAOPHV010000442         2334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_64686_length_2334_cov_2.755595, whole genome shotgun sequence.
ACCESSION   DAOPHV010000442 DAOPHV010000000
VERSION     DAOPHV010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64686_length_2334_cov_2.755595"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..1000
                     /locus_tag="PLQ29_10580"
     CDS             287..1000
                     /locus_tag="PLQ29_10580"
                     /inference="COORDINATES: protein motif:HMM:NF026198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HPG87133.1"
                     /translation="MPLSITALRNHLASCEKPYYVRSSNAGTITYDDLLDRLAEGRTT
                     ITKPDAMAAIQLLMMEMDRALADGFVVKLPFGAFYLVASGTLDAQDQPFEYGDKEYGH
                     DIRLHFRAEREGEKSLAARAELERAAIFDHQLPIVLAFESVYDDASERTVGQGQYLRI
                     HGQRLKFDPTDADLGISFRNGTEHRSPHYISVADQNVVALLPMDIPPGEYSIVTRTRP
                     TGAEVKESVTPGLVVLAAG"
     gene            complement(1661..1879)
                     /locus_tag="PLQ29_10585"
     CDS             complement(1661..1879)
                     /locus_tag="PLQ29_10585"
                     /inference="COORDINATES: protein motif:HMM:NF020542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1902 domain-containing protein"
                     /protein_id="HPG87134.1"
                     /translation="MEYRINAIWDGDARVWIASSDDIPGLTLESGSLDALIERVRTAV
                     PELLHLNGGEAEPVPLFFTSERHDWVAV"
     gene            complement(2046..>2334)
                     /locus_tag="PLQ29_10590"
     CDS             complement(2046..>2334)
                     /locus_tag="PLQ29_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87135.1"
                     /translation="LYGELCPQTGDGGSTEGQSSDSEGQSSASVGSPEPVLVASNGNL
                     VYASGRRVVLVGVDDLVVVDTPDALLVARRGDTQRVKDAVEALKEIDPGLL"
BASE COUNT          489 a          755 c          623 g          467 t
ORIGIN      
        1 tttaatctct cttccttagc gtctccgcgc ggcgtatcgt caaaacatac gatttctata
       61 gtttttttaa cgatttccat tcaagttttt cttttctata cgccgataca gatagggtac
      121 tccggagcgc ggtcttgcta ctatgacggc agtaatgccg ggcggcccct taggagagcc
      181 gctatcacta ctcgcgcgga tcggtcgccg ggttctatac cataacgcag cttagttcac
      241 tgttccactg gctagccgac ggcggccacc gtggaagggc tgacgaatgc ctctgagcat
      301 tacagcgctt cgcaaccacc tcgcctcgtg cgagaaaccc tactacgttc gctcgtcgaa
      361 cgcgggcacc atcacctacg acgatctcct cgatcgcctg gcggaagggc gcaccacgat
      421 caccaagccg gacgcgatgg cggccatcca gctcctgatg atggagatgg acagggccct
      481 ggcggacggc ttcgtcgtca agctgccttt cggcgccttc tacctcgtcg cctcgggcac
      541 gctggacgcc caggaccagc cgttcgaata cggcgacaag gagtacgggc acgacatccg
      601 cctgcacttc agggccgagc gcgagggaga gaaatccctc gccgcgcggg ccgagctcga
      661 gcgagcggcc atcttcgacc accagctgcc catcgtcctc gcattcgagt cggtctacga
      721 cgacgcgagc gagcggaccg tggggcaggg ccagtacctg cgcatccacg gccagcggct
      781 caagttcgac ccgaccgacg ccgacctcgg catcagcttc aggaacggca ccgagcaccg
      841 cagtccccac tacatctcgg tagccgacca gaacgtcgta gcgctcctcc cgatggacat
      901 accgccggga gagtactcca tcgtcacccg gacccgcccc acgggcgcgg aggtcaagga
      961 gagcgtcacc cccggcctcg tagtcctggc ggccggatag tcaacaggta aaaaccagtt
     1021 cggcgccctt cccggcaggg gagggcgccc cttcggacgc gcgacccacg aacgcgctaa
     1081 gcgttcttcc cgggaacgct acgagcgttt gaaacggaat caacgaccat ggagcgttgc
     1141 atatcgctta catccgcacc agacgctcgc gatatccgga ccggacgcta caagcttcct
     1201 accaggacgc ttattgcgtc cctcgccgat tcgcttgtta ccgaacgcga gcccaacact
     1261 aagccgccaa gcctcgaaga tcgctaagaa gacggaacat gatgaccacg atggagcagg
     1321 attaccacga tgaattccta cgtttgtggc ctagtcctta gctctcatca tggtcatctt
     1381 gaggaatccc gtgccatcag gttctgtttc ccgtggtaca ggccctcttc ctaagcgcct
     1441 tctctcgagg ccttgtgtac gagcggctag aaatgaaaat caattcccgc ctgcttcatg
     1501 atagcgttag ccgtatgacg ggattttatc ttaccgtcta cagtaaaatg tatatttgat
     1561 atatcgctat atcaaatatc atgatcgccc ctgccgtggc gaataaaacg gcaaccgcgt
     1621 tccgccaaga tcgcacgtac ccgtttctcg tactccgcca tcagacggcg acccaatcat
     1681 gtcgctccga ggtaaagaaa agggggacgg gctccgcttc gccgccgtta agatgtagca
     1741 attcgggaac agccgtacga acgcgctcga taagggcgtc tagggacccc gactcgagcg
     1801 tcaagccagg aatatcgtcg ctcgacgcga tccagacccg agcgtcgccg tcccatatcg
     1861 cattgattcg gtattccata acgccaccct ccttgaatcg tccggagaaa acggaacaga
     1921 atgaaggcta gaggttaata tttgacctca tccttcatcc ataattttgg tcatcctggg
     1981 acatcctgga catcatgttc cgttttccat agtacaggcc ctactcctta gcgtatccgt
     2041 tgcttctaca gcaatcccgg gtcgatttcc ttgagcgcct ccacggcgtc cttgacgcgc
     2101 tgcgtatcgc cgcgcctggc cacgagcagg gcgtcgggcg tatcgaccac caccaggtcg
     2161 tccaccccga cgagcacgac ccgccgcccg gaagcgtaga ccaggttacc gttcgacgcg
     2221 acgagcacgg gctcagggga gccgaccgaa gcggagctct gtccctcgga atcagagctc
     2281 tgtccctctg ttgatcctcc gtccccggtt tggggacaga gctcgccgta gagc
//
LOCUS       DAOPHV010000443         2330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_64865_length_2330_cov_3.498022, whole genome shotgun sequence.
ACCESSION   DAOPHV010000443 DAOPHV010000000
VERSION     DAOPHV010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64865_length_2330_cov_3.498022"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PLQ29_10595"
     CDS             <1..1033
                     /locus_tag="PLQ29_10595"
                     /inference="COORDINATES: protein motif:HMM:NF006412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPG87136.1"
                     /translation="EIAELCAEELPKVGGKFIQMEDEIGSVAAIIGASLTGRKAMTAT
                     SGPGFSLMQELLGYAAISEVPIVIVDVQRVGPSTGMPTSLAQGDVMQARWGTHGDHPV
                     VVLAPGNVRECFDLTVKAFNIAEEYRVPVIVLSDETIGHMREKVVLNHLKTLKVVNRA
                     APATPAGYVPYKADPDGGVPLMAPFGKGYRWHVTGLSHDETGFPTNKGDVAGALIQRL
                     MRKVEKAEPVLRDYATESADDCDVLVVAFGSSAMSATSAVRQARAKGVKAGLWRPKTV
                     WPFPEEPLRELASKVKRIIVPEMNLGQLVYEVERVVAGKAPVEKLSKVNCELFRPEEI
                     LASIMGGSK"
     gene            1030..1890
                     /locus_tag="PLQ29_10600"
     CDS             1030..1890
                     /locus_tag="PLQ29_10600"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPG87137.1"
                     /translation="MSSGLDYFRQNRLPHIWCPGCGHGTVTGALTRTLAKLGIDKNNV
                     VVVSGIGCSSRASGYLDFDTLHTAHGRAIAFATGIKLSRPELKVIVMTGDGDCTAIGG
                     NHFIHAARRNVDITTIVMNNNNYGMTSGQYSPMTPTGMLGTTAPYGNVERPFDLANLA
                     IASGATFVARGTSYHVQLLGEMIEKALLNPGFSVVEAVVGCPTYYGRKNKIGGAVELF
                     NWQKEHAVNVKAAAAMAPEKLEGKFLIGELYNKAAPEFTAEYARLVEGVRRKFDDFQG
                     GVRGCAQNSD"
     gene            1869..>2330
                     /locus_tag="PLQ29_10605"
     CDS             1869..>2330
                     /locus_tag="PLQ29_10605"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPG87138.1"
                     /translation="MRTEFRLSGSGGQGLLLAGIVLAEGAILDGKNAVQTQSYGPEAR
                     GGSSKAEVVVSEDDIDYPKATDPDFLLALTTDAYKAYGTLMGKGLIIVDSSVCLDPAI
                     AARTVAVPILDTAATTIGKKVVANIVALGVLGGVSGVVRQATLEKAVLNRVP"
BASE COUNT          432 a          782 c          751 g          365 t
ORIGIN      
        1 cgagattgcc gagctgtgcg ccgaagagct tcccaaggtc ggcggcaagt tcatccagat
       61 ggaggacgag ataggctcgg tcgccgccat cattggcgcc tcgctgaccg gtcgcaaggc
      121 catgaccgct accagcggcc ccggcttctc gttgatgcag gagctgctcg gctacgcggc
      181 catctccgag gtgcccatcg tcatcgtcga cgtgcagcgc gtcggcccgt cgaccggcat
      241 gcccacctcg ctggcccagg gagacgtgat gcaggcccgt tggggtactc acggcgacca
      301 tcccgtcgtg gtgctagctc ccggcaacgt gcgcgagtgc ttcgacctga ccgtcaaggc
      361 tttcaacatc gccgaggaat accgcgtgcc ggtcatcgtg ctttccgacg agacgatcgg
      421 gcacatgcgc gagaaggtcg tgctgaatca cctcaagacc ctcaaggtag tcaaccgagc
      481 ggccccggcg actcccgcgg gctacgtacc ctacaaggcc gacccggacg gcggcgtgcc
      541 cctcatggcg ccgttcggca aaggctaccg ctggcacgta accggcctgt cgcatgacga
      601 gaccggcttc ccgaccaaca agggagacgt ggccggcgcc ttgatccagc gcctgatgcg
      661 caaagtcgag aaggccgagc cggtcctgcg cgactatgcg accgagagcg ccgacgactg
      721 cgacgtgctc gtcgtcgcgt tcggctcatc ggccatgtcc gccacctccg cggtgcgtca
      781 ggccagggcc aagggcgtga aggccggcct ctggaggccg aagaccgtct ggccgttccc
      841 cgaggaaccg ctccgcgagc tggcttccaa ggtgaagcgg atcatcgtgc ccgagatgaa
      901 cctgggccag ctcgtgtacg aggtggagcg ggtcgtggcg ggcaaggctc cggtggagaa
      961 gctcagcaag gtcaattgcg agctgttcag gccggaggag atactggcat ccatcatggg
     1021 aggctcgaaa tgagcagcgg cctcgactac ttcaggcaga acaggcttcc tcatatatgg
     1081 tgccccggct gcggccacgg caccgtcacc ggcgcgctga ccaggacgct cgccaagctg
     1141 ggcatcgaca agaacaacgt cgtcgtcgtc tccggtatcg gctgctccag ccgcgcctcg
     1201 ggctacctgg acttcgacac gctccatacc gcccacggcc gcgccatcgc gttcgccacc
     1261 ggtatcaagt tgtcgcgccc cgagctcaag gtcatcgtga tgaccggtga cggcgactgc
     1321 accgccatcg gcggcaacca tttcatccac gcggcccgac gcaacgtcga catcacgacc
     1381 atcgtgatga ataataacaa ctacggcatg acgagtggcc agtactcgcc gatgacgccg
     1441 accggcatgc tcggcacgac ggcgccctac ggcaacgtgg agcggccctt cgacctggcc
     1501 aacctggcca tagcctcggg cgccaccttc gtcgcccgtg gcacttccta ccacgtacag
     1561 ctgctcggcg agatgatcga gaaggccctg ctcaacccgg gcttctccgt ggtcgaggcg
     1621 gtcgtcggtt gcccgaccta ctacggccgc aagaataaga tcggcggcgc ggtcgagctg
     1681 ttcaactggc agaaggagca cgcggtcaac gtaaaggccg cagccgcgat ggcgcccgag
     1741 aagctcgagg gcaagttcct gatcggcgag ctgtacaaca aggcggcgcc ggagttcacc
     1801 gccgaatacg ccaggctcgt cgagggcgtg cggcgtaagt tcgatgattt ccagggggga
     1861 gtacggggat gcgcacagaa ttcagactaa gcggctcggg aggccagggc ctcctgctcg
     1921 ccggcatcgt gctcgccgag ggcgccatcc tggacggcaa gaacgccgtc cagacccaga
     1981 gctatgggcc cgaggcccga ggcggctcca gcaaggccga ggtcgtcgtc tccgaggacg
     2041 atatagacta tcccaaggcc accgacccgg acttcctgct cgcgctgacg accgatgcct
     2101 acaaggccta cggcacgctg atgggcaaag gcctgatcat cgtcgattcg tcggtatgcc
     2161 tcgacccggc catcgccgcg cggaccgtcg ccgtgccgat cctcgacacc gcggcgacga
     2221 cgataggcaa gaaggtcgtc gcgaacatcg tcgccctcgg cgtgctcgga ggcgtatccg
     2281 gcgtcgtcag gcaggccact ctcgagaagg cggtgctcaa ccgcgttccg
//
LOCUS       DAOPHV010000444         2324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_65105_length_2324_cov_4.529749, whole genome shotgun sequence.
ACCESSION   DAOPHV010000444 DAOPHV010000000
VERSION     DAOPHV010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65105_length_2324_cov_4.529749"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1911
                     /locus_tag="PLQ29_10610"
     CDS             <1..1911
                     /locus_tag="PLQ29_10610"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HPG87139.1"
                     /translation="RAELPNWPLNSVAEAFARRALTDPEAGERRSLTRAFVAAERGRM
                     AAMIGALPGLTPLRSMANYYLVRVDAGLAGLSTSDLADRLAVMGVGVRRCGNYDGLSD
                     DYLRVAVRSPGENDAIASAFASALRGGTAARPGPRAAGRRAKALMIQGCSSGAGKSLV
                     AAAFCRIFRDEGYDVAPYKAQNMSLNSAVTPDGLEIGRAQAVQAAACGLEPDARMNPV
                     LLKPESDTGSQVVLLGRPYARRSARDYYATHAELSAAARRAYDELASERELIVLEGAG
                     SPAEINLKSRDFVNMNAAIHAGARVLLVGDIDRGGVFASFIGHVATFSPAELGLLSGF
                     VVNKFRGDQALLGDAFAMTRERTGYPVLGCVPMIRGLAIPDEDEPIVKAGRRPGAELR
                     VAVPRLRRVSNFTDLDAFAIEPDVEIVPVSSSEEFDYGRFDAVIIPGSKSTVDDLGWL
                     RASGLAETIVRFAASGGAVAGICGGYQMLGLSLLDPDGVESPSRETPGLGLLPVVTSF
                     AADKTLSRSSARWVGSGLPEARDAALVGYEIHHGATRPVDTASAEPVILSDGGAPLGY
                     GSGRVWGGYLHGVFDADGFRRAFLNELLARKGLAPLAPSSGPSLDAELSRLAEAVRAN
                     VDLPYIRAELGL"
     gene            1908..>2324
                     /locus_tag="PLQ29_10615"
     CDS             1908..>2324
                     /locus_tag="PLQ29_10615"
                     /inference="COORDINATES: protein motif:HMM:NF015164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HPG87140.1"
                     /translation="MSAPDPASLHAALAIAGGLVLDAVIGDPAAPWHPVRLLGRLAAG
                     AESLARRVLKARPVLAGASAWLAVVAVAAGSALALGRAARAVHPLAGLAVDTLVVWAS
                     IAPADLSGHAARVRRALEAAERGEPGALERARSAVSM"
BASE COUNT          294 a          896 c          798 g          336 t
ORIGIN      
        1 cgggccgagc tgccgaactg gccgctcaat tcggtcgccg aggccttcgc caggcgggcc
       61 ctgaccgacc cggaggccgg agagcgccgc tcgctaaccc gcgccttcgt cgcggccgag
      121 cgcgggcgca tggccgccat gatcggagcc ctgcccggac tgaccccgct ccgttccatg
      181 gccaactact acctcgtgcg cgtcgacgcg gggctcgccg gcctatcgac gtccgacctg
      241 gccgatcgcc ttgccgtcat gggcgtcggg gtccgccgct gcggcaacta cgacgggcta
      301 tccgacgact atctgcgcgt cgccgtccgc tcgcccggcg agaacgacgc catagcgtcg
      361 gccttcgcct cggcgctgcg aggagggacg gccgcccggc ccggcccgag ggctgccgga
      421 cgccgggcca aggcgctgat gatccagggc tgttcctccg gcgcgggcaa gagcctcgtc
      481 gcggccgcct tctgccgcat cttccgcgac gagggctacg acgtggcgcc gtacaaggcg
      541 cagaacatgt cgctcaactc cgcggtgacg cccgacggcc tcgagatcgg ccgggcccag
      601 gccgtccagg cagccgcctg cggcctcgag ccggacgcgc gcatgaatcc ggtgctgctc
      661 aagcccgaga gcgacacggg ttcccaggtc gtactgctcg gcaggcccta cgcgaggcgc
      721 tcggcccgcg actactacgc gacgcacgcc gagctgagcg ctgcggcccg ccgtgcctac
      781 gacgaactgg cctctgaacg cgagctgatc gtgctcgagg gcgccggcag ccccgccgag
      841 atcaacttaa agagccgcga cttcgtcaac atgaacgcgg ccatccacgc cggcgcccgc
      901 gtcctcctgg tcggcgacat agaccgaggc ggcgtcttcg cctcgttcat cggccacgtg
      961 gctaccttct cgccggcaga gctcggcctc ctgtccggct tcgtcgtcaa caaattccgc
     1021 ggcgaccagg ccctgctcgg cgacgccttc gcgatgacgc gggaacggac cggctacccc
     1081 gtgctcggct gcgtgccgat gatacgcggc ctcgccatac ccgacgagga cgagcctatc
     1141 gtcaaggccg gccgacgccc cggcgccgag ctgcgcgtcg cggtgccgag gctacgccgc
     1201 gtgtcgaatt tcaccgacct tgacgccttc gccatcgagc ccgacgtcga gatcgtgccc
     1261 gtctcctcga gcgaggagtt cgactatggc cggttcgacg cggtgatcat ccccgggagc
     1321 aagtcgaccg tcgacgacct cggctggctc cgcgcgtcgg gccttgccga gacgatcgta
     1381 cgattcgccg cctccggcgg cgctgtagcg gggatatgcg gcggctatca gatgcttggg
     1441 ctgtccctgc tcgaccccga cggggtcgag tcgccgtccc gcgagacgcc ggggctcggg
     1501 ctcctgcccg tggtcacgag cttcgcggcc gacaagacgc tgtcgcgctc gagcgcgcgc
     1561 tgggtcggct ccggcctacc cgaggcccgg gacgccgcgc tggtcggcta cgagatccat
     1621 cacggcgcga cgaggcccgt cgatacggcc tcggccgaac ccgtgatcct gtccgacggc
     1681 ggcgcgccgc tcggctacgg ctctgggcgc gtctggggcg gatacctgca cggcgtcttc
     1741 gacgcggacg gcttccgccg cgcgttccta aacgagctgc tcgcccgaaa aggactcgcg
     1801 ccgctggcgc cgagctccgg cccgagcctc gacgccgagc tgtctcgcct cgcggaggcc
     1861 gtccgcgcca acgtcgacct gccctacatc cgcgcggagc tcgggctgtg agcgcgcccg
     1921 atcccgcgtc gctccacgcg gccctggcca tcgcgggcgg cctcgtcctc gacgccgtga
     1981 tcggtgaccc ggccgcgccc tggcacccgg tacggctgct cggaaggctc gccgccggcg
     2041 ccgagtcgct ggccaggcgc gtcttgaagg cgcgccccgt tctagccgga gcctccgcct
     2101 ggctggccgt cgtcgccgta gcggccggct ccgccctcgc cttgggccgg gcggcccgcg
     2161 ccgtacatcc cctggcgggc ctcgccgtcg acacgctcgt cgtctgggcc tcgatagcgc
     2221 cggcggacct gtccgggcac gcggcccgcg tcaggcgcgc gctcgaggcc gccgagcgcg
     2281 gcgagccagg agcgctcgaa cgtgcccgct cggccgtatc gatg
//
LOCUS       DAOPHV010000445         2322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_65199_length_2322_cov_4.246581, whole genome shotgun sequence.
ACCESSION   DAOPHV010000445 DAOPHV010000000
VERSION     DAOPHV010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65199_length_2322_cov_4.246581"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..686
                     /locus_tag="PLQ29_10620"
     CDS             225..686
                     /locus_tag="PLQ29_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87141.1"
                     /translation="MRRTTPLIAIALLVSVAAASGQAIVAPDDPATLLGLTPAQAIER
                     FGPPGSVFAIRGAEPWQDDVVFDYGGGFSLFLFKDRVWQVRIAEPYARPVLGLVVGSG
                     VERAISALGAPALALGGSYEWALPGEAWPVRLRGVTDGGRIRELYIYRADF"
     gene            695..2221
                     /locus_tag="PLQ29_10625"
     CDS             695..2221
                     /locus_tag="PLQ29_10625"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HPG87142.1"
                     /translation="MPNICLCLTGSTIAQDLEALERYRDKVDMVELRADYLDPAELFH
                     LRFFPEKAGMPSILTVRRKSDGGKFDLGEGVRLVTFARGLAFADSDHRKNFSYVDIEH
                     DFHIPSIQEAARTFGTKIIRSKHCLDGMPADIDKVWRELSSRPSEIPKLAVKANGMAD
                     VDSVFRLFEGGQGRGERVVVSMGEYGFCTRVLADRLGSMLVYASATGAGLEAPAPGQL
                     DPSRLLEVYRAREFEQDWSLYGILGGPAVIHSLSPIIHNAGFSALGLRSLYLPFPSDD
                     VESFLSLADRLSIRGFSITVPFKERVLPYLSRRSAEVEVIGACNTAIKDGSEWVGYNT
                     DAYGFTADVRRFLGVDTLRGLKATIVGAGGAAKAAAYALNSLGAKVCVVNRSMSKARR
                     LAEAYGFEWSGMTERAADLMERYNDLIVQTTSVGLEGGQDGDPIEWYEFSGREALYEA
                     IYSPPETQVMRRARKAGCRVTNGLGMLYAQAEAQFSLFSGHDYPKGGQPCPNSIDQPS
                     "
     gene            2191..>2322
                     /locus_tag="PLQ29_10630"
     CDS             2191..>2322
                     /locus_tag="PLQ29_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPG87143.1"
                     /translation="MPEQHRSAFVAVVGRPSAGKSTLVNRICGQKVAIVSPVPQTTRN
                     "
BASE COUNT          393 a          787 c          752 g          390 t
ORIGIN      
        1 tgcgcggaag atggcccggc attcctggag cagcgcgacg tccgcagtag cgcgaacctc
       61 ggagagcgca tcgtcgatct cggcctcgat ggccgcccga cgggattctg ggagtatcga
      121 atgagatgac acgtatgact ccttagacgt aaacataatc gaaaggcccc tactttctca
      181 acccaggctt gcgacgcgtc tccggctccg atacagtatc ggggatgcgc cgaacaacgc
      241 ccctcatagc gatcgccctc ctcgtatccg tcgcggccgc ctccggtcag gcgatagtcg
      301 ccccggacga tcccgccacg ctactgggcc tgacgccggc ccaggccatc gaacgcttcg
      361 ggccgcccgg gagcgtattc gcgatacgcg gcgccgagcc ctggcaggac gatgtcgtct
      421 tcgactacgg cggcggattc tcgctattcc tcttcaagga tagggtctgg caggtcagga
      481 tcgccgagcc gtacgccagg ccggtcctcg gcctcgtcgt cggctccggc gtcgaacggg
      541 cgatctccgc gctcggggcc ccggccctcg cgttgggcgg gtcgtacgaa tgggccttgc
      601 cgggagaggc ctggccggtt aggctcaggg gcgtgaccga cggaggacgt atccgcgagc
      661 tttatatcta ccgagctgac ttctaggagc ggcgatgccg aatatttgcc tgtgcctgac
      721 gggatcgacc atcgcccagg acctggaggc gctggaacgc taccgcgaca aggtggacat
      781 ggtcgagcta cgcgccgact acctcgaccc ggccgagctg ttccacctgc gcttcttccc
      841 ggagaaggcc ggcatgccga gcatcctcac ggtcaggcgc aaatccgacg gcggcaagtt
      901 cgaccttgga gagggcgtac ggctggtcac gttcgccagg ggactcgcgt tcgccgactc
      961 ggaccatcgc aagaatttct cgtacgtcga catcgaacac gattttcata tcccctcgat
     1021 ccaggaagcg gcgcgtacct tcggcaccaa gatcatccgc tcgaagcact gcctcgacgg
     1081 tatgcccgcc gatatcgaca aggtctggcg ggagctgtcg tctcggccgt cggagattcc
     1141 gaagctggcg gtcaaggcga acggcatggc cgacgtcgac tcggttttca ggcttttcga
     1201 gggcggccag ggccgcggcg agcgggtcgt cgtcagcatg ggcgagtacg gcttctgcac
     1261 gcgggtgctc gccgaccggc tcggcagcat gctcgtctac gcgagcgcca ccggggcagg
     1321 gctcgaggcg ccggcgcccg gccagctcga cccgtcccgc ctgctcgagg tctaccgggc
     1381 cagggagttc gagcaggact ggagcctcta cggcatactc ggagggccgg ccgtcatcca
     1441 ttcgctgtcg cctatcatcc ataacgccgg tttctccgcg ctcgggctac gatcgctcta
     1501 cctgccgttc ccgagcgacg acgtcgagag cttcctgtcg ctcgccgaca ggctgtcgat
     1561 acgcggcttc tcgatcaccg tgccgttcaa ggagcgcgtc ctgccctacc tgtccaggcg
     1621 atccgcggag gtcgaggtca taggggcgtg caatacggcc atcaaggacg gatcggaatg
     1681 ggtcggctac aacaccgacg cctacggttt caccgccgac gtccgcaggt tcctcggcgt
     1741 cgatacgctg cgcggcctaa aggcgacgat agtcggcgcc ggaggggccg ccaaggcggc
     1801 cgcctacgcg ctgaactcgc tcggcgccaa ggtctgcgtc gtcaaccgca gcatgtccaa
     1861 ggccaggcgc ctcgccgagg cctacggctt cgagtggtcc ggcatgaccg agcgcgcggc
     1921 cgacctcatg gagcgctaca acgacctgat cgtgcagacc acctcggtcg ggctcgaggg
     1981 cgggcaggac ggcgacccta tcgaatggta cgaattctcc ggccgcgagg cgctctacga
     2041 ggccatctac agcccgccgg agacgcaggt gatgcgccgc gcccgcaagg ccggctgccg
     2101 cgtgactaac ggcctcggca tgctctacgc ccaggccgag gcccagttct cgctcttctc
     2161 cggccatgat tacccgaaag gagggcagcc gtgcccgaac agcatcgatc agccttcgta
     2221 gccgtcgtcg gaaggccgtc ggccggcaag tcgaccctcg tcaacaggat ctgcggccag
     2281 aaggtggcca tcgtctcgcc cgtgccgcag accacgcgca ac
//
LOCUS       DAOPHV010000446         2321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_65266_length_2321_cov_3.080759, whole genome shotgun sequence.
ACCESSION   DAOPHV010000446 DAOPHV010000000
VERSION     DAOPHV010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65266_length_2321_cov_3.080759"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLQ29_10635"
     CDS             <1..449
                     /locus_tag="PLQ29_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPG87144.1"
                     /translation="AGLYAELSLFACSLGAYFSLLAFRDVSFRRCLFLSPLLDMERLI
                     RNMMGWAGVDESRLREEGEIVTGFGETLSWNYYCYAVEHPVDRWPSQTSILYGEHDDI
                     TDRATLDAFIARFGCDVTVEPGGEHYFHTPGQLDALRAWTSSRVIP"
     gene            complement(823..>2321)
                     /gene="htpG"
                     /locus_tag="PLQ29_10640"
     CDS             complement(823..>2321)
                     /gene="htpG"
                     /locus_tag="PLQ29_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPG87145.1"
                     /translation="FYASYMVADEVTVTSRKAGQDGAWVWKSDGKGSYDIEPGERDGH
                     GTTVTLKMNDDGREYLSRWRLEELVKKYSNHVAYPIRLAYDEDEYDDKGKKKGSKAHK
                     DEQVNSAQALWRRPKAELKDEDYVGFYKNIADSDEEPLLWTHTKAEGTIEYTTLFYIP
                     SKAPFDLYHADYRPGVKLYVRRVFITDDDKELLPTYLRFVRGVVDSEDLPLNVSREIL
                     QQNRVMSSIRNASVKKILSELKSLSENDKDTYAAFVAEFNRPLKEGLYGDFANREALQ
                     ELVRFKSSSVDGWTSLADYKARMGDEQKAIYYIAGDSEERLRKSPLLEMYRKRGVEVL
                     ICPDEIDDIVMPMVGKYGDLELKAVNRSGAEKEFEDKLDESKAKDLAPVIERVKKALG
                     DEVKDVRLSARLSESPSCIVVDADDPSIQLQQMMKSMGRGGDFEVKPILEINGDHALV
                     ARLAASDDEALASDVARVLLDQALLVEGVALKDPSDFVARINRLIADV"
BASE COUNT          351 a          750 c          735 g          485 t
ORIGIN      
        1 ccgccggcct gtatgccgag ctgtcgctct tcgcctgcag cctcggcgcc tatttcagcc
       61 tgctcgcctt ccgagacgta tccttccggc gctgcctctt cctgtcgccg ctcctcgata
      121 tggagcggct gatacggaac atgatgggct gggcgggcgt cgacgagtcc cgcctccgcg
      181 aggagggcga gatcgttacc ggcttcggag agacattgtc ttggaattat tactgttacg
      241 ccgtcgagca tcccgtcgac cggtggccga gccagacctc gatcctgtac ggcgagcacg
      301 acgatatcac cgatcgagcc acgctcgacg ccttcatcgc ccgtttcggc tgcgacgtga
      361 ccgtcgagcc gggcggcgag cattacttcc atacccccgg gcaactcgac gcgctccggg
      421 cgtggacctc gtcgcgcgtc ataccgtaat cggcaagctt agtcttcgaa gcttgtgagc
      481 cgactcgtcc aaacactata gcgcgtagca cgggcttcgc ggcgctaccc tgcatggtgg
      541 taatagagaa agcaccacaa gcttcgagga actactaaaa gcaagcggcg agccgccccg
      601 cgactcgccc gcttgaacgc cctatattct ctctacgtta agcggcgaac cgcgcagcgg
      661 ctcgtccgat tgaatgaaat tccccgcatc gccgcgtcgt catccgtgac gttcccgtcc
      721 tgcggacggg ccgttgtcgg gcgcgccctc catggcgcgc gtatcacgtc ggcgatgcgg
      781 tcttacccta cttgatcgcg gcgcgtagcg cgcctggcgc gcttacacgt ccgcgatcaa
      841 gcgattgatg cgcgccacga agtcggaggg gtccttgagg gcgacgccct cgacgagcag
      901 ggcctggtcg agtagcacgc gggccacgtc ggaggccagg gcctcgtcgt cggacgcggc
      961 caggcgggcg acgagcgcgt ggtcgccgtt gatctcgagt atcggcttga cttcgaagtc
     1021 gccgccgcgt cccatgctct tcatcatctg ctggagctgg atgctcgggt cgtcggcgtc
     1081 gacgacgatg cacgacgggc tctcggacag gcgggccgat aggcgcacgt ccttgacctc
     1141 gtcgccgagg gccttcttga cgcgctcgat cacgggggcc aggtctttag ccttgctctc
     1201 gtcgagcttg tcctcgaact ccttctcggc gccggagcgg ttgacggcct tgagctcgag
     1261 gtcgccgtac ttgccgacca taggcatcac gatgtcgtcg atctcgtcgg ggcagatcaa
     1321 cacctccacg ccgcgtttgc ggtacatctc gagcagcggc gacttgcgta gacgctcctc
     1381 ggagtcgccg gcgatgtagt agatcgcctt ctgctcgtcg cccatgcggg ccttgtagtc
     1441 ggccagcgat gtccagccgt cgaccgagct gctcttgaag cgcacgagct cctgcagggc
     1501 ctcgcggttg gcgaagtcgc cgtacaggcc ctccttgagc ggtcggttga actcggcgac
     1561 gaaggccgcg tacgtgtcct tgtcgttctc cgagaggctc ttgagctcgg acagtatctt
     1621 cttgaccgac gcgttgcgta tcgagctcat cacgcggttc tgctgcagta tctcgcggct
     1681 cacgttgagc ggcaggtcct cggagtcgac gacgccgcgg acgaagcgca ggtaggtcgg
     1741 caggagctcc ttatcgtcgt cggtgatgaa cacgcggcgc acgtacagtt tgacgccggg
     1801 cctataatcg gcgtggtaca ggtcgaacgg cgccttggac gggatataga acagggtcgt
     1861 gtactcgatg gtgccctcgg ccttggtatg ggtccagagc aggggctcct cgtctgaatc
     1921 cgctatgttc ttgtagaacc cgacgtagtc ctcgtccttg agctcggcct tggggcgtcg
     1981 ccagagggcc tgggcgctgt tgacctgctc gtccttgtgc gccttggagc ccttcttctt
     2041 acccttatcg tcgtactcgt cctcgtcgta ggccaggcgt atcgggtagg ccacatggtt
     2101 cgagtacttc ttgacgagct cctcgaggcg ccagcggctc aggtactcgc ggccgtcgtc
     2161 gttcatcttg agcgtgacgg tcgtgccgtg gccgtcgcgc tcgccgggct cgatatcgta
     2221 ggagcccttg ccgtcgctct tccataccca ggcgccatcc tggccggcct tgcggctcgt
     2281 gacggtcacc tcgtcggcga ccatgtagct ggcgtagaag c
//
LOCUS       DAOPHV010000447         2319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_65332_length_2319_cov_3.633392, whole genome shotgun sequence.
ACCESSION   DAOPHV010000447 DAOPHV010000000
VERSION     DAOPHV010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65332_length_2319_cov_3.633392"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PLQ29_10645"
     CDS             <1..859
                     /locus_tag="PLQ29_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HPG87146.1"
                     /translation="AAATGARAMTSSSSPGVSLKQEGISYACGADVPMVIVNVARSGP
                     GLGGISPAQGDYFQSTRGGGHGDYHHIVLAPKSVQEATDLTFEAFDLADEWRMPAMIL
                     ADGMIGQMMEGVTLPEAKDPATRKPKPWAVGNKGKRTELNHVDSTQLVPENFEKALHV
                     RYARYDEIKKTVVRWEEQNLEDADLILSAYGTSARVCLGAMQMARKLGLKVGLLRPIT
                     LWPFPEQRFGALAGKRFLVVEMSMGQMVEDVRLYTHGKSEVSFYGRCGGMIPTEEDVL
                     AEIRRILKK"
     gene            870..1640
                     /locus_tag="PLQ29_10650"
     CDS             870..1640
                     /locus_tag="PLQ29_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPG87147.1"
                     /translation="MELVYGHPKSLKPVQTKYCPGCGHGVIHRLITEVIDEMGMQEKS
                     IILNPVGCSIWASLYFDFDSVQVPHGRTPAGATAVKRMLPDHLVICYQGDGDLAAIGT
                     AEAVHAANRGEKFTTIFVNNAIYGMTGGQMAPTTLVGQKATTAPYGRDPVEAGMGYPI
                     RVLEMLATLGGTKYLARGAVNNAANVRKTKQYIKKAFEAQMRGEGSSMVEILSQCPTN
                     WAMDPVDSVGWVESAMMPFYALGEVKNELAGAGKEAGK"
     gene            1637..2173
                     /locus_tag="PLQ29_10655"
     CDS             1637..2173
                     /locus_tag="PLQ29_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPG87148.1"
                     /translation="MTEKTFMAGFGGQGVISMGQLWVYCGMKEDLEVTFFPFYGAEKR
                     GGIARASVIVSTEEIASPLVTRPDSAIVMNMDSLPLCEANLKQGGTMIINSSLVKVET
                     KRTDARVIKVPCNDIAEKLGDVKFANMVMMGALSKATGAVKLDKLEAVLMSFFGPSKQ
                     KFIPMNLKAIEEGMKAVG"
BASE COUNT          446 a          771 c          749 g          353 t
ORIGIN      
        1 ggccgccgcg accggcgccc gcgcaatgac cagctcgtcg agccccggcg tgtcgctcaa
       61 gcaggagggc atctcctacg cctgcggcgc cgatgtaccg atggtcatcg tcaacgtggc
      121 gcgctccggc cccggcctcg gcggcatctc gccggcccag ggcgactact ttcagtcgac
      181 ccgcggcggc ggccacggag actaccacca catcgtgctg gcgcccaaga gcgtccagga
      241 ggcgaccgac ctgaccttcg aggccttcga cctggccgac gagtggcgca tgcccgcgat
      301 gatcctggcc gacggcatga taggccagat gatggagggc gtcacgctgc ccgaggccaa
      361 ggacccggcg acgcgcaagc ctaagccctg ggccgtcggc aacaagggca aacgaacgga
      421 gctcaaccac gtcgactcga cccagctggt ccccgagaat ttcgagaagg ccctgcacgt
      481 gcgctacgcg cgctacgacg agatcaagaa gaccgtcgtg cgctgggaag agcagaacct
      541 cgaggacgcg gacctcatcc tgagcgccta cggtacctcg gcccgcgtct gcctgggcgc
      601 gatgcagatg gccaggaagc tcggactcaa ggtcggcctc ctccggccga taacgctgtg
      661 gccgttcccg gagcagcgct tcggcgcgct ggccggcaag cgcttcctgg tcgtcgagat
      721 gagcatgggg cagatggtgg aggacgtgcg cctgtatacg cacggtaaga gcgaagtttc
      781 gttctacggc cgctgcggcg gcatgatccc gacggaagag gacgtactcg ctgaaatccg
      841 ccggatcctg aagaagtaag ggggcgagcg tggaactcgt atacggacat cccaagagcc
      901 tcaagccggt ccagaccaag tactgccccg gctgcggcca cggcgtcatt caccgcctga
      961 taaccgaggt catcgacgag atgggcatgc aggagaagtc gatcatcctg aacccggtcg
     1021 gttgctctat ctgggcgagc ctgtacttcg acttcgactc ggtgcaggtg ccgcacggcc
     1081 gcacgccggc cggcgccacc gccgtcaagc gcatgttgcc cgaccacctg gtcatctgtt
     1141 accagggcga cggcgacctg gccgccatcg gtaccgccga ggccgtgcac gccgccaacc
     1201 gcggcgagaa gttcacgacc atcttcgtga acaacgccat ctacggcatg accggcggcc
     1261 agatggcccc gaccaccctc gtcggccaga aggccaccac cgcgccctac ggccgcgacc
     1321 cggtcgaggc cggcatgggc taccctatcc gcgtgctcga gatgctcgcc accctcggcg
     1381 gtaccaagta cctggcccgc ggcgccgtga acaacgccgc caacgtgcgc aagaccaagc
     1441 agtacatcaa gaaggccttc gaggcccaga tgcgcggcga gggctcgagc atggtcgaga
     1501 tcctgtcgca gtgccctacc aactgggcga tggaccccgt cgacagcgtc ggctgggtgg
     1561 agagcgcgat gatgccgttc tacgcgctcg gcgaggtcaa gaacgaactg gcgggcgcgg
     1621 gcaaggaggc cggtaaatga ccgagaagac tttcatggcc ggcttcggcg gccagggcgt
     1681 gatatcgatg gggcagctgt gggtatactg cggcatgaag gaggacctcg aggtcacctt
     1741 cttcccgttc tacggcgccg agaagcgcgg cggcatcgcc cgcgccagcg tcatcgtctc
     1801 caccgaggag atcgccagcc cgctcgtcac gaggcccgac agcgccatcg tgatgaacat
     1861 ggattccctg cccctctgcg aggcgaacct caaacagggc ggcacgatga tcatcaactc
     1921 gagcctggtc aaggtcgaga ccaagcggac cgacgcccgc gtcatcaagg tgccgtgcaa
     1981 cgatatcgcc gagaagctgg gcgacgtcaa gttcgccaac atggtgatga tgggcgcctt
     2041 gtcgaaggcg accggcgcgg tcaagctcga taagctcgag gccgtgctca tgtccttctt
     2101 cggcccgtcg aagcagaagt tcatcccgat gaacctcaag gcgatcgagg aaggtatgaa
     2161 ggccgtcggc tgacgcctcg accctgaacc gaacgacccc gaggcctggc ctcggggttt
     2221 ttttgcgcta tatagagagg catgatgcag cgacaccgct atgtacgaac cacctggagc
     2281 ttcgcgactc tcgccgccgc cctggttctc ggctcttgc
//
LOCUS       DAOPHV010000448         2291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_66398_length_2291_cov_4.223614, whole genome shotgun sequence.
ACCESSION   DAOPHV010000448 DAOPHV010000000
VERSION     DAOPHV010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66398_length_2291_cov_4.223614"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLQ29_10660"
     CDS             <1..205
                     /locus_tag="PLQ29_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87149.1"
                     /translation="LGVMGLVGRTRTDKTTGTVMMAAGALGIASFVLKGFTGFILGAA
                     GIGLVGFGVFNLFKFVKGLKSRG"
     gene            277..1590
                     /gene="miaB"
                     /locus_tag="PLQ29_10665"
     CDS             277..1590
                     /gene="miaB"
                     /locus_tag="PLQ29_10665"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPG87150.1"
                     /translation="MRFFIETYGCQMNKAESASFHLQFMERSWEPAATAEEADLVLIN
                     TCSVRITAENRAWGRIAHYAAMKRERAFTLVVTGCMAERLRERMREREPRIDYALGNF
                     QKAAFGLLLDAAAAGRRLETIEESPAFAFMPSHREPGSFSSFVPIMHGCDNFCSYCIV
                     PYVRGREVSRDPSSIAAEIESAAAAGVREITLLGQNVNSYRWRGLGGELDFAGLLTSI
                     APIARSAGIGRVRFVSSHPKDLSDATIEAIASDDVFARHIHLCVQHGSDRVLEAMNRR
                     YTRDSYTLLADRIRSRISGVTLSTDILIGFPGETEEDVEDTLELMRKLRFAYAFMYHY
                     NPREGTPAAVMPGQVPVAVKKARLARVIEAQKTITRSVMDGMIGSEAVVLVEAVSKRR
                     GDEVLGRTDQDMMVVFPASAERIGSFARVRLESIRGNTFKAVEVR"
     gene            1587..1952
                     /locus_tag="PLQ29_10670"
     CDS             1587..1952
                     /locus_tag="PLQ29_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87151.1"
                     /translation="MMPWKLLAFIAVMAIVLVFIGFNLDNRCDISLVVVTFTSVPVVI
                     TILVTYILGLLSALVLSIGRRPSSSRRGIPLRQVAPSVSEQPGADGEAPFKPSASSSS
                     DTQPAGSGSKRPSKGKKRG"
     gene            1957..>2291
                     /locus_tag="PLQ29_10675"
     CDS             1957..>2291
                     /locus_tag="PLQ29_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87152.1"
                     /translation="MMRSSVPAIALLTIVVLTMVPLRAKCQGAEAVAALERLTASASA
                     PDLAARARTEFATLSRIDEVAWLAGALAAKAVVPSQRGNLLVEQASALELLGRYGEAS
                     VVWEVAATAV"
BASE COUNT          399 a          716 c          727 g          449 t
ORIGIN      
        1 gctcggggtc atgggtctcg ttggccggac gcgtaccgat aagaccaccg gcacggtcat
       61 gatggccgcc ggtgcgctcg gcatagcctc gttcgtgctc aagggcttca ccggcttcat
      121 cctcggcgca gccggcatcg gcttggtggg cttcggcgtg tttaacttgt tcaaattcgt
      181 caaggggctc aagtccaggg gctaagctcc gtcgatgctc tgacctcaac gtgggcggcc
      241 gctcagtcgg ccgcccgatt catatccggg gagatcatgc gtttttttat agagacctac
      301 ggttgccaga tgaacaaggc cgagtcggcg tcgttccatc ttcagttcat ggagagaagc
      361 tgggagccgg cggctacggc ggaagaggcc gacctcgtcc tgatcaatac ctgctcggtg
      421 cgcatcaccg ccgagaatcg cgcttggggt cgcatagccc attacgcggc gatgaaacgc
      481 gagcgcgcct tcacgctggt cgtaaccggt tgtatggcag agcggctcag ggagcgcatg
      541 cgagagcgcg agcctaggat agactacgcg ctcggcaact tccagaaggc ggccttcgga
      601 cttctgctcg acgcggccgc cgccggacgg cgactcgaga cgatcgagga gtcgccggcc
      661 ttcgccttca tgccatcgca tcgcgagccg ggctcgttca gctccttcgt cccgatcatg
      721 cacggctgcg ataacttctg ctcgtactgc atcgtgccct atgtccgagg gcgggaagta
      781 tcgcgcgacc cgtcgtcgat agccgccgag atagaatcgg ccgccgcagc gggcgttcgc
      841 gagatcacgt tgctgggcca gaacgtcaac tcgtaccgat ggcgcggtct gggcggcgag
      901 ctcgacttcg cgggcctcct gacgtctatc gcccccatag ccaggtcagc cggcataggc
      961 cgcgtccgct tcgtctcgag ccatcccaag gacttgtccg acgcgacgat agaggcgatt
     1021 gcgtcagacg acgtcttcgc caggcacata cacctgtgcg tccagcacgg ctccgatcgc
     1081 gtgctcgagg cgatgaaccg taggtacacg cgcgactcct atacgctctt ggccgatcgt
     1141 atccgctcgc gtatatcggg cgtcaccctt tcgaccgata ttctcatagg attccccggc
     1201 gagaccgaag aggacgtcga agataccctg gagctgatgc gcaagctacg cttcgcctac
     1261 gccttcatgt accattacaa tccgcgtgag ggcacgcccg ccgcggttat gcctgggcag
     1321 gtacccgtgg ccgtcaagaa ggccaggctg gcccgcgtca tagaggcgca gaagacgata
     1381 acgaggagcg tcatggacgg catgatagga tcggaagctg tcgtgctcgt cgaggctgtc
     1441 tcgaaacgaa gaggcgacga ggtgctcggg cgtaccgacc aggacatgat ggtcgtcttc
     1501 ccggcgtcgg ccgagcgtat cggttcgttc gccagggtac ggctcgagtc gatacgaggg
     1561 aatacattca aggccgtgga ggtgcgatga tgccctggaa gttactggct ttcatagcgg
     1621 tgatggcgat cgttctggtg ttcatcgggt ttaacctgga taatcgctgc gatatatcgc
     1681 tggtagtcgt caccttcacg tctgtaccgg tggtaataac catcctggtc acctatatac
     1741 tggggctgct atcggccctg gtcctctcga taggcaggcg cccctcgtcg tcgaggcgcg
     1801 gcatcccgct caggcaagtc gcgccgtccg tttccgagca gccaggcgcg gatggcgagg
     1861 cgccgttcaa gccgtcagcc tcatcctcct ccgatacgca acctgccgga tccggttcga
     1921 aacgcccctc gaagggaaag aagagaggct gagctcgtga tgcgctcctc cgtgccggcg
     1981 atcgcacttc tgaccatcgt cgtcttgacg atggtcccgc tccgcgccaa gtgccaaggc
     2041 gccgaagccg tcgcagcgct cgagcgcttg accgcttcgg cctcagcccc cgacttggcg
     2101 gcgcgggctc gaaccgagtt cgctacgctg agccgaatcg acgaagtagc ctggttggcc
     2161 ggtgccttgg ccgccaaggc cgtggttccg tcccaacgtg ggaaccttct cgtcgagcaa
     2221 gcctccgcgc tcgagcttct ggggcgctac ggcgaggcct cggtcgtatg ggaggttgcg
     2281 gccaccgccg t
//
LOCUS       DAOPHV010000449         2288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_66533_length_2288_cov_3.639498, whole genome shotgun sequence.
ACCESSION   DAOPHV010000449 DAOPHV010000000
VERSION     DAOPHV010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66533_length_2288_cov_3.639498"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..372)
                     /locus_tag="PLQ29_10680"
     CDS             complement(88..372)
                     /locus_tag="PLQ29_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HPG87153.1"
                     /translation="MDDTQGTPTRVEGVEYPVEFEMRVIYLADCRDELTLALERTLTE
                     RGAALERPRELPAKGAKYGRLACRVRFDDQESLYAAYAAIGELPGVKAAL"
     gene            complement(390..1880)
                     /gene="sbcB"
                     /locus_tag="PLQ29_10685"
     CDS             complement(390..1880)
                     /gene="sbcB"
                     /locus_tag="PLQ29_10685"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HPG87154.1"
                     /translation="MAASILWYDLETFGLDPKHDRIAQFAGLRTNDELEPISDKLVLY
                     ERIAPDYLPSPYSCLVHRISPRRTLEEGIPEYEFAKAIRAEMLAPGTCVAGYNSMRFD
                     DEFVRNLLYRNLFDPYEREWADGNTRWDVLDLFRATRDLRPDGMSWPDGPDGKPDFRL
                     VGLAQANGVPLESAHDAFHDIGATIGLTRLVKRTQPRLYEWYWRHRTRDSLRPLVNLA
                     DREPLVHTAAQYTRPEGCTTIVAPVGMVPGRRDGLVAIDLRFDPSPLVDLSVDEIRRR
                     VFSRKSELADGERIPLVDIRLGRCPYLAPLATMDAGAADRLGLDRGLAIERAGSLARE
                     PELIQKLLAVFAPRAPEPMERDPDYRIYSGGFFRDEDKDAMAAVHEAIATLGPSEARP
                     QAYAMPFIDERLPQLVRRMFARNWPGALSPGEAARWRSFCAGRLLCPRIEGAVDMAGF
                     SKTVESLLGNLDTPAEDKPILLELLEYRRSLEREVLSYEKEGTSRT"
     gene            1997..>2288
                     /locus_tag="PLQ29_10690"
     CDS             1997..>2288
                     /locus_tag="PLQ29_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPG87155.1"
                     /translation="MRDERALLEKYLRDRGLKMTRARETVLDAFLGLESHVSVEAVIE
                     AARRLDPGVGQATVFRTVKLLVDAGLAREACHDDGSRLFEHAYNHAHHDHLTC"
BASE COUNT          349 a          757 c          795 g          387 t
ORIGIN      
        1 gcctgccgcc aggtgcggtg gctcgcgtcg cggagccgcc cgcggctcgc gtcggcccgc
       61 cagagccgga cctccgcctg tcttcgctca cagcgccgcc ttgacgccgg gcaactcgcc
      121 tatggcggcg taggcggcat acagcgattc ctgatcatcg aaacggaccc tacaggccag
      181 cctgccgtac ttggctccct tggccggtag ctcgcgcggc cgttcgaggg ccgccccccg
      241 ctcggtcagc gtccgttcga gggctagcgt cagctcgtcc ctgcaatcgg cgaggtagat
      301 gacccgcatc tcgaactcga ccggatactc gacgccctcg acgcgcgtag gcgtcccctg
      361 cgtatcgtcc atagggttac tctccttgat catgtccggc tcgttccttc cttctcgtac
      421 gacagcacct cccgctcgag ggagcgtcgg tactcgagca gctcgagcag gatcggcttg
      481 tcctcggcag gcgtgtcgag gttgccgagt agcgattcga ccgtcttgga gaagccggcc
      541 atgtcgacgg cgccctcgat gcgggggcag agcaggcggc cggcgcagaa ggatcgccat
      601 cgggccgcct ctccgggcga tagggcgcct ggccagttcc tggcgaacat gcgcctgacc
      661 agctggggca ggcgctcgtc gatgaacggc atggcgtagg cctggggccg agcctcggac
      721 gggccgagcg tcgctatggc ctcgtgcacg gccgccatcg cgtccttgtc ctcgtccctg
      781 aagaagccgc ccgagtagat gcggtagtcg gggtcgcgct ccatcggctc cggcgcacgg
      841 ggcgcgaaca ccgcgagtag cttctggatc agttccggtt ctcgagccag gctcccggcc
      901 cgctcgatcg ccaggccccg gtccagcccg agccggtccg cggccccggc gtccatcgtc
      961 gccagcggcg ccaggtaggg gcagcggccc aggcggatgt cgacgagggg gatgcgttcg
     1021 ccgtcggcca gctcggactt cctggagaag acccggcggc gtatctcgtc gacggacagg
     1081 tcgacgagcg gcgacgggtc gaagcggagg tcgatggcga cgagcccatc ccggcggccc
     1141 ggcaccatgc cgaccggcgc gacgatggtc gtgcagcctt ccggcctcgt gtactgggcg
     1201 gcggtatgga cgaggggctc gcggtcggcc agattgacga ggggccgcag cgagtctcgc
     1261 gtgcggtggc gccagtacca ttcgtacagc cgcggctggg tgcgcttgac cagcctcgtc
     1321 aggcctatcg tcgcgccgat gtcgtggaac gcgtcatggg ccgattcgag cggcacgccg
     1381 ttggcctggg ccaggccgac cagcctgaag tccggcttgc cgtcggggcc gtccggccac
     1441 gacatgccgt ctggacggag gtctctcgtc gcccggaaca ggtcgagtac gtcccagcgc
     1501 gtgttgccgt cggcccattc gcgttcgtac gggtcgaaga ggttgcggta gagcaggttg
     1561 cgcacgaact cgtcgtcgaa gcgcatcgag ttatagccgg ctacgcaggt gcccggggcg
     1621 agcatctcgg cgcgtatcgc cttggcgaac tcgtactcgg gtatgccctc ctcgagcgtc
     1681 ctgcgaggcg agatgcggtg gacgaggcag gagtaggggc tcggcaggta gtccggggct
     1741 atgcgctcgt agaggacgag cttatcggat atcggctcga gctcgtcgtt cgtccggagc
     1801 ccggcgaact gcgctatcct gtcgtgcttg gggtcgaggc cgaaggtctc gagatcgtac
     1861 catagaatgc tcgcggccat ggggcggagt ataccctagg gcgcgcgggc gagtgaagcg
     1921 cgaaataaac cttgacagcc ctgtctgtaa atgataaccc ttatcaatag acttgtttcc
     1981 gtgaatggag tacgccttgc gcgatgagag ggccctactg gagaagtacc tacgggatcg
     2041 cggcctcaaa atgacccgag cgcgggagac cgtgctcgac gctttcctcg gcctggaatc
     2101 ccacgtcagc gtcgaggcgg tcatcgaagc cgcccggcgc ctcgacccgg gcgtcggcca
     2161 ggcgaccgtt ttcaggaccg tcaagctgct ggtcgacgcc gggctggccc gcgaggcctg
     2221 tcacgacgac ggcagccgcc tgttcgagca cgcgtacaac cacgcccatc acgaccacct
     2281 gacctgca
//
LOCUS       DAOPHV010000450         2278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_66976_length_2278_cov_4.460189, whole genome shotgun sequence.
ACCESSION   DAOPHV010000450 DAOPHV010000000
VERSION     DAOPHV010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66976_length_2278_cov_4.460189"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLQ29_10695"
     CDS             <1..732
                     /locus_tag="PLQ29_10695"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPG87156.1"
                     /translation="RRYAAGPGLVQAGATVPEPSMDIQSVLPPSLLAERLESLAYLYK
                     RADSAVDAFVAASGVSCPFGCGSCCEGFVPDILPVEAAYLAAYLAAADRPKAYEAAAG
                     RLGVRSYPDGRQGCPLYRTDTPYHCGVYEARPLICRMFAFSATRDKLSMTAFSVCRLG
                     SSRNGARGARGEALIDAFGAEPPVMGDYGSELAGIEPESAGRRMPLPEALAEAASRVM
                     FLIGIRDDNPLDSGPDVRPPLPNAS"
     gene            838..1992
                     /locus_tag="PLQ29_10700"
     CDS             838..1992
                     /locus_tag="PLQ29_10700"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPG87157.1"
                     /translation="MPDRRRRPSDGRPSSVALLALQRGLTGDRALAGSGYMDDPDYLA
                     AYLEYYRAVSMAQVGRIVDLSGIAPRSVLDLGAGPGSVSLELLGRGVRRFFLVDSSRA
                     ALDAATASIRESAGRLGVQVDIETRVADLESPDAIPEGRRFDLAAFGHCLNEIGKGDD
                     RFSRRRDVVDRAARALAPGGSVMLLEPATLAASRDALALRDALVSDGWTIEAPCTLCG
                     LCPALAAGPNHTCHDEAAWEVPTGVRRLAEAAGLDRELIKMTWYVVSASSRRVSASSR
                     RVSASLDHVSASSNHGPTASGDFGEGYYRVVSAPMLNKGGRVRYLLCGPSGRFPFSAR
                     QDDAAARSAGFFELGRYDLITVDKPERRENGWGFGPGTRLRRLPPGAERG"
     gene            2002..>2278
                     /locus_tag="PLQ29_10705"
     CDS             2002..>2278
                     /locus_tag="PLQ29_10705"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HPG87158.1"
                     /translation="MAMRLASFIGWSNTGKTGFVEACAAALSARGVGVAAVKCVRHGG
                     SFNLEGKDTSRFFAAGAETALVAAEETVRVSRTPPDWGRAWVESLFPS"
BASE COUNT          292 a          816 c          779 g          391 t
ORIGIN      
        1 cgtcgatacg cggctggacc cggcctcgtt caggccggcg ccaccgtccc ggagccttct
       61 atggacattc agagcgtcct ccccccgtcg ctgctcgcgg agcggctcga gagcctggcc
      121 tacctgtaca agcgggccga ttcggccgtc gacgccttcg ttgccgcctc cggcgtcagc
      181 tgcccctttg gctgcgggtc ctgctgtgag ggcttcgtgc cggatatcct gcccgtcgag
      241 gccgcctacc tggccgccta cctggccgcg gcggaccggc ccaaggccta tgaggcggcc
      301 gccggccggc tcggggtccg ttcctatccc gacggccgcc aaggttgccc gctgtacagg
      361 accgacacgc cgtaccattg cggcgtctat gaggcgcggc cgttgatctg caggatgttc
      421 gccttctccg ccaccaggga taagctgtcc atgacggctt tctccgtttg cagacttggc
      481 tcgagccgga atggcgctag gggggcgcga ggcgaggctt tgatcgacgc cttcggcgcc
      541 gaaccgccgg tcatgggcga ttacggctcg gagctggccg gcatcgagcc tgagtccgcg
      601 gggcgcagga tgcccctgcc cgaggctttg gccgaggcgg catcacgggt gatgttcctg
      661 ataggcatcc gtgacgacaa tccgctcgac agcgggccgg acgtcaggcc gcccctgccg
      721 aacgcgagct agtggccccg tcccggggcg gcgcttgcca tcgtcgtccc ccgctggtat
      781 cgtcgacggc gatgaaacgt tccactcgcg caagttcggc gtcaggcgcc aggtccgatg
      841 cctgaccgcc gccgtcgacc atccgacggc cgcccgagct ccgtggccct gctggccctg
      901 caacgcggcc tgaccggcga ccgggccttg gccggctcgg gctacatgga cgatcccgac
      961 tacctcgcgg cttacctcga gtactaccgc gccgtgtcga tggcccaggt cgggcggatc
     1021 gtcgacctgt cgggcattgc gccgcgttcg gtgctcgatc tgggcgccgg gcctggttcg
     1081 gtgtcgctcg agctgctcgg gcgcggcgtc cgccggttct tcctggtcga ctcgtcgcgc
     1141 gcggccctcg acgcggcgac cgcctcgata cgcgagtcgg ccggccgcct cggcgttcag
     1201 gtcgacatcg agacgcgggt cgccgacctg gagtcgcccg acgccatacc agaaggccgt
     1261 cgcttcgatc tggcggcgtt cgggcactgc ctcaacgaga taggcaaggg cgacgaccgg
     1321 ttctcacgaa ggagggacgt cgtcgatcgg gccgcccgcg cgctggcgcc cggcggatcc
     1381 gtcatgctgc tcgagccggc cacgctcgcg gcgagccgcg acgccctggc cttgcgcgac
     1441 gccctcgtct cggacggctg gaccatcgag gcgccctgta cgctatgcgg gctctgcccg
     1501 gccctcgccg ccggcccgaa ccatacctgc cacgacgagg ctgcctggga ggtgcctacc
     1561 ggcgtcaggc gcctggccga ggcggccggc ctcgaccgag agctcatcaa gatgacctgg
     1621 tacgtcgtct cggcttcgtc gagacgtgtc tcggcttcgt cgagacgtgt ctcggcttcg
     1681 ttggaccatg tttcagcttc gtcgaaccac ggtccgacgg cgtctggaga cttcggagag
     1741 gggtattatc gcgtcgtgtc ggcgccgatg ctcaacaagg gcggccgcgt gcgctacttg
     1801 ctctgcggcc catccggacg cttcccgttc tccgcgcgcc aggacgacgc ggccgcccgg
     1861 agcgccggct tcttcgagct cgggcgctac gatctcataa ccgtagacaa gcctgagcgg
     1921 cgcgagaacg gctggggctt cggtcccgga acccgcctac ggcgcttgcc tcccggcgcg
     1981 gagcgaggct aggatacggc catggctatg cgcttagcgt cgttcatagg ctggtcgaac
     2041 accggcaaga ccggcttcgt cgaggcctgc gccgccgccc tctccgcgcg cggcgtcgga
     2101 gtcgccgccg tcaagtgcgt acggcacggc ggcagcttca acctcgaagg caaggatacc
     2161 tcgcgctttt tcgccgccgg ggccgagacc gccctcgtag ccgcggagga gactgtacgc
     2221 gtctcgagga cgcctcccga ttggggccgc gcctgggtcg agtcgctgtt cccctccg
//
LOCUS       DAOPHV010000451         2277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_67038_length_2277_cov_3.105311, whole genome shotgun sequence.
ACCESSION   DAOPHV010000451 DAOPHV010000000
VERSION     DAOPHV010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67038_length_2277_cov_3.105311"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /gene="nrdD"
                     /locus_tag="PLQ29_10710"
     CDS             <1..914
                     /gene="nrdD"
                     /locus_tag="PLQ29_10710"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPG87159.1"
                     /translation="NSDLDPGDVRSMCCRLQLDKRELRKRGGGLFGSDELTGSIGVVT
                     LNMPRLGYLADGKSDFYRRLDRLMDLAAKSLSIKRKVCSRLLEGGLFPYTRRYLRTFD
                     NHFSTIGLVGMNECCLNFMGKSIAEPDGKAFAEEVLEHMRKRLQDYQVDTGELFNLEA
                     TPAESTSYRLAKHDRKRYPDIISAGAEEPYYTNSTQLPADFTDDLFDALDHQESLQSK
                     YTGGTVFHVFLGEAVDDWRALRDLVKTVAARYRIPYYTISPTFSVCPVHGYISGEHFS
                     CPKCKAEKEARIREEISVLENELNGPS"
     gene            957..1484
                     /gene="nrdD"
                     /locus_tag="PLQ29_10715"
     CDS             957..1484
                     /gene="nrdD"
                     /locus_tag="PLQ29_10715"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPG87160.1"
                     /translation="METRESKEARLAALKAELLCVEGTTTEVYSRIVGYYRSVKNWNA
                     GKRAEFGKRREYAFPSVAQSAVEPAVQPVQPADALLGSTRSGAPSSYMLFTRAACPNC
                     PPVRDYLRSSGLPGVVVDVDTEEGLELARRYEVMSTPTAILMGSGGEQLGRAHARSQL
                     ERLVKPELELEVATA"
     gene            1487..2191
                     /locus_tag="PLQ29_10720"
     CDS             1487..2191
                     /locus_tag="PLQ29_10720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPG87161.1"
                     /translation="MRLGIQKTSLVDFPGRVAAVLFTAGCPFRCPYCHNPELVEVSCD
                     GDTEGDGLSSVEEALAFIEHRKPVLSGVVISGGEPTIHAELPALAAAVKRLGLFVKLD
                     TNGCYPERIAAVGADYVAMDLKTTPERYGELWPGAPADAADRIRTAVRAVRGSGAEYE
                     FRITCAPEFVSEIEAEAIARLLEPDDAVYLQRYRPGRTLDPAWAAKVSPYPDERLERI
                     LAIVRRAAPKARLRGF"
BASE COUNT          402 a          769 c          738 g          368 t
ORIGIN      
        1 tcaactcgga cctcgatccc ggcgatgtgc gctcgatgtg ctgccgcctc cagctcgata
       61 agcgggagct tcgaaagcgc ggcggcggcc tgttcggctc ggacgagctg accggctcga
      121 tcggcgtcgt caccctgaac atgccgcgcc tcggctacct ggccgacggc aagagcgact
      181 tctaccgcag gctcgaccgg ctgatggacc tggccgccaa gagcctatcg atcaagcgca
      241 aggtatgctc gaggctgctc gagggcgggc tcttcccgta tactaggcgc tacctgcgca
      301 ccttcgacaa tcacttctcg accataggcc tcgtcggcat gaacgagtgc tgcctgaact
      361 tcatgggcaa gagcatcgcc gagcccgatg gcaaggcctt cgccgaggag gtcctcgagc
      421 acatgcgcaa gcgcttgcag gactaccagg tcgacacggg cgagctgttc aacctggagg
      481 cgaccccggc cgagagcacc tcgtatcgct tggccaagca cgaccgcaag cgctatcccg
      541 atatcatatc ggccggtgcg gaggagccgt actacaccaa ctcgacccag ctgcccgccg
      601 atttcaccga cgacctgttc gacgcgctcg accaccagga atcgctacag tcgaagtaca
      661 ccgggggcac ggtgttccac gttttcctcg gcgaggccgt cgacgactgg cgggcgctcc
      721 gcgacctcgt caagacggtg gccgcccgct accgcatacc ctactatacg atatcgccga
      781 ccttctcggt ctgccccgtc cacggctaca tctccggcga gcatttcagc tgcccgaagt
      841 gcaaggccga gaaagaggct cggatcaggg aagagatatc cgtgctcgag aacgaactga
      901 acggcccgtc ctgacaggcg ccgccgatat atgtactgac atcctgggag ggaacgatgg
      961 agacgaggga gagcaaagag gcaaggctgg cggccctcaa ggccgagcta ctgtgcgtcg
     1021 aggggacgac caccgaggtg tacagccgca tcgtgggcta ttaccgttcg gtcaagaact
     1081 ggaacgccgg caagcgggcc gaattcggca agcgccggga atacgccttt ccctcagtcg
     1141 cccagtcagc cgtcgagccg gccgttcagc ccgttcagcc cgccgacgcg ctcctgggct
     1201 cgaccaggtc cggcgcgcct tcgtcgtaca tgctattcac gagagccgcc tgcccgaact
     1261 gtccgccggt gcgcgactac ctccgctcga gcggcctccc cggcgtggtc gtcgacgtcg
     1321 ataccgagga aggcctcgag ctggccaggc gctacgaggt catgtcgacg ccgaccgcca
     1381 tcctgatggg ctcgggcggc gagcagctcg gtagggccca cgcccgctcc cagctcgagc
     1441 ggctggtcaa gcctgagctc gaactcgagg tggcgacggc gtagcgatgc gcctgggcat
     1501 acagaagacc agcctcgtcg atttccccgg acgcgtggcc gccgtgctgt tcacggccgg
     1561 ttgcccattc cgttgcccgt attgccataa ccccgaactg gtcgaggtga gctgcgatgg
     1621 ggataccgag ggtgacggcc tctcgtcggt agaggaggct ctggccttca tcgagcaccg
     1681 caagccggtg ctatcgggcg tggtcatctc cggcggcgag ccgacgatcc atgcggagct
     1741 accggccttg gccgccgccg tcaagcggct tgggttgttc gtcaagctcg acacgaacgg
     1801 ttgctatccc gaacgcatcg cggctgtcgg cgccgactac gtagcgatgg acctgaagac
     1861 gaccccggag cgatacggcg agctgtggcc cggagcgccg gccgacgcgg ccgataggat
     1921 caggaccgcc gtgcgcgcgg tgcgcggctc gggggcggag tacgagttca ggattacctg
     1981 cgcccccgag ttcgtctccg agatcgaggc cgaggcgatc gccaggctgc tcgagcccga
     2041 cgacgccgtc tacctgcaac gctaccgtcc ggggcgcacg ctcgacccgg cctgggcggc
     2101 caaggtctct ccgtaccccg acgagcggtt ggaacgcatt ctggccatcg tccgacgagc
     2161 agctcccaag gctaggctca ggggcttctg agccattccg tcgccgcgtc cgcgatgccg
     2221 tcgtagtcgt cggccccgaa ccagcgcacg ccggggatgg cgcgcatgaa ggtgagc
//
LOCUS       DAOPHV010000452         2275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_67117_length_2275_cov_3.344595, whole genome shotgun sequence.
ACCESSION   DAOPHV010000452 DAOPHV010000000
VERSION     DAOPHV010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67117_length_2275_cov_3.344595"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(308..2074)
                     /locus_tag="PLQ29_10725"
     CDS             complement(308..2074)
                     /locus_tag="PLQ29_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPG87162.1"
                     /translation="MVNAKINGIPVQVAEGTTILEAARKVNVRIPTLCYNDDLPPWAA
                     CGICIVKVEKNPKMLRACCTPIEEGMSVITHDPEIVQVRKTVIELILSTHPDDCLQCP
                     RNGSCELQTLAAEFGVREQPFEKRLRDLPIDASTGSLILNPEKCVRCGRCVLVCQQMQ
                     NVWAIEFLGRGESTRIAPAADVKLAEGPCIKCGQCSAHCPVGAIYENDQTKEVWDALM
                     KGGDDAKTCVVQIAPAVRVALGEEFGLEPGAVITKKIYAALRRLGFDRVFDTNFSADL
                     TIMEEGTEFVKRFTDYAAGKDKGMLPLITSCCPAWVDYMEKYYDDMIPNFSTAKSPQQ
                     MMGAMIKTFWAEQAKVDPKKIYSVSIMPCTAKKWECGRNDDMWSSGEKDVDVVLTTRE
                     LARMIKQAGIDLVNLPEEEADSPLGPYTGAGTIFGTTGGVMEAALRSAYYLVTKEDLK
                     DVNFTGVRGISGIKEARIHIKGIEVRVAVAHQMGNIAAVLEEVRKAKAEGKDVPWHFI
                     EVMACRGGCIGGGGQPYGATDEVRQKRMAGLYTDDEKSTLRCSHHNPAIQYAYKEFLG
                     EPNGHKAHELLHTSYKKLELYK"
     gene            complement(2087..>2275)
                     /locus_tag="PLQ29_10730"
     CDS             complement(2087..>2275)
                     /locus_tag="PLQ29_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPG87163.1"
                     /translation="CKDLVVYSIDKSKCIGCGLCARKCPVNCITGDKTQKFTIDASKC
                     IKCGECFKACKFSAVVKK"
BASE COUNT          379 a          730 c          712 g          454 t
ORIGIN      
        1 catatccggc ctccgtcgcc tatactatac acaaacccac ccggtacgat acgccgcagg
       61 accgcgccag cgatccgagc cggcgtatcg ttcaccgaac cgccccgcca gcaccggcca
      121 cacaacatac cggcagtaaa cccgcccgga acgatacgcc gcagaatcgc gaagcgatcc
      181 gagccggcgt agcgttccgc ctaaaaataa gaacgcccga gccgcgaggc tcgggcgaat
      241 catagaaacc caggacgtta agccgtaaaa accgcgaagc ggtttttatc ggcttaaacg
      301 tccgccgtta cttgtacagt tccagcttct tgtagctcgt gtgcaggagc tcgtgcgcct
      361 tgtgcccgtt gggctcgccg aggaactcct tgtacgcgta ctggatagcc gggttgtggt
      421 gcgagcagcg cagcgtgctc ttctcgtcgt cggtgtacag gccagccatg cgcttctgcc
      481 tgacctcgtc ggtcgcgccg tagggctgac cgccgccgcc gatacaaccg ccgcggcagg
      541 ccatcacctc gatgaagtgc catggaacat ccttgccctc ggccttggcc ttgcggacct
      601 cctcgagcac cgcggcgatg ttgcccatct ggtgggccac cgccacgcgg acctcgatgc
      661 ccttgatgtg gatgcgagct tccttgatgc cggagatgcc gcggacgccg gtgaagttga
      721 cgtccttgag gtcttccttg gtgacgaggt agtaggcgga acggagcgcc gcctccatga
      781 cgccgccggt cgtaccgaag atcgtgccgg cgccggtgta cggaccgagc gggctgtcgg
      841 cctcttcctc gggaagattc acgaggtcga tgccagcctg cttgatcatg cgggccagct
      901 cgcgggtcgt cagcacgacg tccacgtcct tctcgccgga gctccacatg tcgtcgttgc
      961 ggccgcactc ccacttcttg gcggtacagg gcatgatcga taccgagtag atcttcttcg
     1021 ggtcaacctt ggcctgctcg gcccagaagg tcttgatcat cgcgcccatc atctgctgcg
     1081 ggctcttcgc ggtggagaaa ttcgggatca tgtcgtcgta gtacttctcc atgtaatcca
     1141 cccaggccgg gcagcaggag gtgatcagcg gcaacatgcc cttgtccttg ccagcggcgt
     1201 agtcggtgaa gcgcttaacg aactcggtgc cttcctccat gatggtcagg tcggccgaga
     1261 agttcgtgtc gaacaccctg tcgaagccga ggcggcgcag cgccgcgtag atcttcttcg
     1321 tgatgacggc gccgggctcg aggccgaatt cctcgccgag cgctacgcgg acggccgggg
     1381 cgatctggac cacgcaggtc ttggcgtcgt cgcccccctt catcagcgcg tcccagactt
     1441 ccttggtctg gtcgttctcg tagatggcgc cgaccgggca gtgggcggag cactggccgc
     1501 acttgatgca cgggccctcg gccagcttga cgtcggcagc gggggcgatg cgggtgctct
     1561 cgccgcgacc caggaactcg atggcccaga cgttctgcat ctgctggcag accagcacgc
     1621 agcggccgca gcgcacgcac ttctccgggt tcaggatcag cgagccggtc gacgcgtcga
     1681 tcggcaggtc gcgcaggcgc ttctcgaagg gctgctcgcg gacgccgaac tcggcggcca
     1741 gggtctgcag ctcgcaggag ccattgcgcg gacactggag gcagtcgtcc gggtgggtcg
     1801 acaggatcag ctcgatcacc gtcttgcgca cctggactat ctccgggtcg tgggtgatga
     1861 cgctcatgcc ctcctcgatc ggggtgcagc acgcgcggag catcttcggg ttcttctcga
     1921 ccttgacgat gcagataccg caggccgccc acgggggaag atcgtcgtta tagcagagcg
     1981 tggggatgcg tacgttgact ttcctggccg cctcgaggat cgtggtgccc tcggcgacct
     2041 gtaccggtat tccgtttatc ttggcgttga ccatggggcc tactccttat ttcttcacga
     2101 cggcgctgaa cttgcaggcc ttgaagcact cgccgcactt gatgcacttc gatgcgtcga
     2161 tggtgaattt ctgcgtcttg tcgccggtga tgcagttgac cgggcacttg cgggcgcaga
     2221 gcccgcaacc gatgcacttg ctcttgtcta tgctgtagac gacgagatcc ttgca
//
LOCUS       DAOPHV010000453         2275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_67129_length_2275_cov_2.697748, whole genome shotgun sequence.
ACCESSION   DAOPHV010000453 DAOPHV010000000
VERSION     DAOPHV010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67129_length_2275_cov_2.697748"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PLQ29_10735"
     CDS             complement(<1..407)
                     /locus_tag="PLQ29_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HPG87164.1"
                     /translation="MALGCKYGTHRVIEPVGAMPQTAQRIDNDMTKVYDNEILLDVIA
                     LNVDSASFTQIEEEAGGDPDKIGKKIMEIVAARGKQQNPVTGSGGMFIGKVARIGSAL
                     KDRDLKVGDKIASLVSLSLTPLKINKILKVMPEV"
     gene            complement(553..924)
                     /locus_tag="PLQ29_10740"
     CDS             complement(553..924)
                     /locus_tag="PLQ29_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87165.1"
                     /translation="MVTDEARLLKELIRKEARLLENSVRNDPAKVGALVDARGVFVSA
                     SGARGALEPGKPFDSLQGVSYIDSNTVELLDLSEDCRLLVYVAATVSKNTRAKSRCSS
                     VWKRDSGDWKVIFHQETTILD"
     gene            complement(991..2271)
                     /locus_tag="PLQ29_10745"
     CDS             complement(991..2271)
                     /locus_tag="PLQ29_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HPG87166.1"
                     /translation="MPRPGTDLERWAVVACDQYSSEREYWEDVSRLVGGKPSTLNLIF
                     PECYLEDDDKAARVERIRSAMRSYLESGKVARQAPGFVLVERATPFEQHPRKGLVIAV
                     DLEAYQYGKGVTSRIRPTEGTIVERLPPRMDIRRGAPLELPHIMLLIDDPKRSVIEPL
                     YAKMDSLPKLYDFDLMKGAGHIRGAMVSDEADLAGVAAALEAIADEGAYRAKYGSSDV
                     LLFAVGDGNHSLATAKAIWEEIKKANASDPELMSHPARHALVELVNIYDEGLPFHPIH
                     RVLFKADERALLADLAAAGAVFEPMDWKKAVAKTDEPSGAEHRFAFVSAGTSGLVRFA
                     KPSANLAAGTIQEVLDAYLKAHAGASIDYIHGTDSLETLARKPGNLGLYLPPIDKSSF
                     FGTVIRDGAMPRKTFSMGEAPEKRFYIEARKIAR"
BASE COUNT          371 a          704 c          766 g          434 t
ORIGIN      
        1 acctcgggca tcaccttgag gatcttgttg atcttgagcg gggtcagcga cagcgacacg
       61 aggctggcga tcttgtcgcc gaccttgagg tcgcgatcct tgagcgccga gccgatcctg
      121 gccaccttgc cgatgaacat gccgccggac ccggtgaccg ggttctgctg cttgccgcgg
      181 gccgcgacaa tctccatgat cttcttgccg atcttgtccg ggtcgccgcc ggcctcttcc
      241 tcgatctgcg tgaagctcgc ggagtcgacg ttgagcgcga tgacgtcgag caggatctcg
      301 ttgtcgtaga ccttggtcat atcgttgtcg atacgctgcg cggtctgcgg catggccccg
      361 accggttcga tgacgcggtg ggtgccgtac ttgcatccta aagccataat gcctccttct
      421 gggtagtttc cggcgcgatc cgagggcgac gtagccggat agatgtacgg atcatacctt
      481 ataaacccgc cccagaggaa gcgtcaagtt acgttaaaat agccgtttca ccaggagcaa
      541 cgatggcctc gattagtcca gtatagtcgt ttcctgatgg aatatgacct tccaatcgcc
      601 cgaatcgcgc ttccagaccg agctacagcg cgacttggcg cgggtattct tactgacggt
      661 agccgcaacg tagacaagca acctgcaatc ctctgatagg tctaggagct cgaccgtgtt
      721 gctgtcgata tacgagacgc cctgcaggct gtcgaacggc ttgcccggct ccaaggcgcc
      781 acgggcgcca gacgccgaga cgaacacgcc gcgcgcgtcg acgagcgcgc cgaccttcgc
      841 cgggtcgttc cggacgctgt tctccagtag ccgcgcttcc ttgcggatca gctctttcag
      901 tagccgcgct tcatcagtga ccatcgcata cccccgggcg gcatcatagc atggaaaacc
      961 ggcccccgtc ccgggggccg gcgagggtcg tcagcgcgcg atcttcctag cctcgatata
     1021 gaagcgcttc tctggcgcct ctcccatcga gaaggtcttg cggggcatgg ctccgtcgcg
     1081 gatgacagtg ccgaagaagc tcgacttatc gatcggcggt agatagaggc cgaggttgcc
     1141 cggcttcctg gccagggtct cgaggctatc ggtgccgtgg atatagtcga tcgaggcgcc
     1201 ggcatgggcc ttgaggtagg cgtccaggac ctcctggatg gtgccggccg ctaggttcgc
     1261 gctcggcttg gcgaagcgga cgaggccgct cgtcccggcc gacacgaagg cgaagcgatg
     1321 ctcggcgccg gaaggctcgt cggtcttggc tacggccttc ttccaatcca tcggctcgaa
     1381 aacggcgccg gcggcggcga ggtcggcgag gagggcccgt tcatcggcct tgaacaggac
     1441 gcggtggatg gggtggaacg gcaggccctc gtcgtagatg ttgacgagtt cgacgagggc
     1501 gtggcgcgcc ggatggctca tcagctcggg gtcggaggcg ttagccttct tgatctcctc
     1561 ccagatggcc ttggcggtgg ccagcgagtg gttgccgtcg ccgacggcga acaggagcac
     1621 gtccgatgag ccgtacttgg cccggtacgc gccctcgtcg gctatcgcct cgagagccgc
     1681 ggccacgccg gccaggtcgg cctcgtcgga gaccatcgcg ccgcggatat ggccggcgcc
     1741 cttcatgagg tcgaagtcgt agagcttggg caagctgtcc atcttggcgt acagcggctc
     1801 gatcacgcta cgcttggggt catcgatgag gagcatgata tggggtagct cgagcggcgc
     1861 gccgcggcgt atgtccatgc gcgggggcag gcgctcgacg atggtgccct cggtcggccg
     1921 gatccggctc gtgacgccct tgccgtactg gtaggcctcg aggtcgacgg cgatgacgag
     1981 gcccttgcgg ggatgctgct cgaacggggt cgcgcgctcg acgagcacga aaccgggcgc
     2041 ctgcctggct accttgcccg actcgaggta ggagcgcatc gcggagcgga tgcgctcgac
     2101 gcgggccgcc ttgtcgtcgt cctcgaggta gcactcggga aagatcaggt tgagggtgct
     2161 cggcttaccg ccgaccagcc tggagacgtc ctcccagtac tcgcgctcgc tcgagtactg
     2221 atcgcaggcg acgacggccc agcgttcgag gtcggtgccc gggcgtggca ggcac
//
LOCUS       DAOPHV010000454         2274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_67154_length_2274_cov_4.001803, whole genome shotgun sequence.
ACCESSION   DAOPHV010000454 DAOPHV010000000
VERSION     DAOPHV010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67154_length_2274_cov_4.001803"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLQ29_10750"
     CDS             complement(<1..313)
                     /locus_tag="PLQ29_10750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPG87167.1"
                     /translation="MAQAIDLGSVARRQDIPELYLSKLATPLKAAGILRSERGAKGGY
                     RLARRPEDITVLDVVEALEGRSSLLECTGEPGACVRSGECKTLPIWSGLDKVIQDYLR
                     GI"
     gene            complement(492..1280)
                     /locus_tag="PLQ29_10755"
     CDS             complement(492..1280)
                     /locus_tag="PLQ29_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HPG87168.1"
                     /translation="MNRKALALSVLSLVATLSFAQAPQPALKVGATPVPHAELLRLVA
                     SELAAQGIKLEVVEFTDYVTPNIALAEGQLDANFFQHVPYLESFSAERKLSLEAAGAI
                     HVEPLGVYSKKYLSLDALPSGATIAIPNDPTNEGRALLLLASKGLITLKPGAGLKATL
                     RDIASNPRKFKFRELEAAQLPRTLADVAASVINGNYAILAGLNPVKDAIALEGAESPY
                     ANVVAVRQGEASDPRIVALVAALRSPKVKEFILATFGGGVVPVF"
     gene            complement(1390..2043)
                     /locus_tag="PLQ29_10760"
     CDS             complement(1390..2043)
                     /locus_tag="PLQ29_10760"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HPG87169.1"
                     /translation="MLELLDLLIRPTLETVAMVAASTLAAVLLGAPVGLFLYLIAPGG
                     LSERPALRRVLDRAINALRSFPFIILMIMLFPLSRILIGTSIGTAAAIVPLAIAAAPF
                     VARLVESALSELDRGVVDAAVASGATKARIALAILLPEALPSLVSGLSLTVINLIGYS
                     AMAGAIGGGGLGDLAVRFGYHRFRLDVMTAAVLVILVLVEGAQAAGNAAVRRIKSMR"
     gene            complement(2036..>2274)
                     /locus_tag="PLQ29_10765"
     CDS             complement(2036..>2274)
                     /locus_tag="PLQ29_10765"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HPG87170.1"
                     /translation="LRFSGASASQPVLSRLSREFPVEVNILRGTIHKVGGSRSGELVV
                     TFQGDDPAIKAAKSWLTAQGIVVESTVVGETDHA"
BASE COUNT          412 a          685 c          786 g          391 t
ORIGIN      
        1 ttatgccgcg taggtaatcc tgtatgacct tatcgagtcc tgaccagatc ggtagcgtct
       61 tgcactcgcc tgaccggacg caggctcccg gttccccggt acactcgagg agggacgagc
      121 gtccctcaag agcctccacg acgtccagga cggtgatatc ctcggggcgg cgggccaggc
      181 ggtagccgcc cttcgcgccg cgctccgacc gcaggatgcc cgcggccttg agcggcgtcg
      241 cgagcttcga caggtacagt tcggggatat cctggcgcct cgcgacgctt ccgaggtcta
      301 tagcctgtgc catacctcgc tcggctaact ccacgagtag ccgtagcccg tatcttgacc
      361 tagtcgttat caagctcata taccatccta aaaagaccta aatagtctac ttatgaaaga
      421 cgattgtgtc aacccatggg tgccgttcac gaaagacggc gccccggcag tgatgacctt
      481 cgctggatcc gttagaacac gggtacgacg ccgccgccga aggtggccag gatgaattcc
      541 ttgaccttgg gcgagcggag ggccgcgacg agggcgacga tgcgcggatc ggatgcttct
      601 ccctggcgta ccgccacgac attagcgtat ggcgactcgg ctccctcgag ggctatggcg
      661 tccttgacgg gattgagccc ggcgagtatc gcgtagttgc cgttgatgac ggacgcggcg
      721 acgtcggcga gggtccgcgg caactgggcg gcctcgagct ccctgaactt gaacttcctg
      781 gggttggaag cgatgtcgcg tagcgtggct ttgagcccgg cgccaggctt cagcgtaatg
      841 aggcccttgg acgcgaggag gaggagcgcg cggccctcgt tcgtcgggtc gttgggaatg
      901 gctatcgtcg cgccgctggg tagcgcatcg agcgataggt atttcttaga atagacgccc
      961 agaggctcga catggatggc gccggctgcc tcgagcgaca gctttcgctc ggcggagaac
     1021 gactccaggt acggcacgtg ctggaagaag ttggcgtcga gctggccctc ggccagggcg
     1081 atgttgggcg tcacgtagtc ggtgaactct acgacctcca gtttgatgcc ctgggcggcg
     1141 agctccgatg cgacgagccg gagcagttcg gcgtgcggaa cgggcgtcgc tccgaccttg
     1201 agcgccggct gcggggcttg agcgaagctt agcgtcgcta cgagcgacag tactgatagt
     1261 gccagggcct tgcggttcat aggaacctcc ttgcgatgag tcgcggtcgc tctacgagcg
     1321 ccgaaaaggg actgtgggga gttaatggag ccgacggacg gggcggcgct tcagccgact
     1381 ctccgccggc tagcgcatgg acttgatcct gcggacggcc gcgttgcccg cggcctgggc
     1441 gccttcgacg aggacgagaa tgacgagcac ggcggccgtc atcacgtcca gtcggaagcg
     1501 atggtagccg aaccgtacgg cgaggtcgcc gagtccgccg ccgcctatgg ccccggccat
     1561 ggccgagtag ccgatcagat tgatcacggt cagcgacagg cccgatacca gtgatggcag
     1621 ggcctcgggc aggagtatgg ccaaggcgat gcgcgccttg gtcgcgcccg aggcgacggc
     1681 cgcgtcgacg acgccccggt ctagctcgga cagcgccgat tcgacgagcc gagcgacgaa
     1741 gggcgcggcg gctatggcca acggcacgat cgcggcggcc gtgcctatgc tggtgccgat
     1801 caggatcctg gaaagaggga acagcatgat catgagtatg atgaacggga acgagcgcag
     1861 cgcgttgatg gcccggtcca gtacgcggcg aagcgcgggc cgctccgaca ggccgcccgg
     1921 agctatcagg tacaggaaga gtccgacggg agcccctagg aggacggcgg ccagcgtcga
     1981 cgcggcgacc atcgctacgg tctccagcgt cggtcgtatg agtaggtcga gcagttcaag
     2041 catggtcggt ctccccgacg accgtcgatt cgacgactat gccttgagcc gttaaccagc
     2101 tcttggcggc cttgatcgca gggtcgtcgc cctggaacgt gacgacgagc tcgcccgagc
     2161 gcgatccgcc taccttgtgt atggtgccgc ggaggatatt gacctcgacg gggaactcgc
     2221 gtgacagacg ggacagcacg ggctgggatg ccgacgcgcc cgagaaacgc aacc
//
LOCUS       DAOPHV010000455         2273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_67188_length_2273_cov_4.051849, whole genome shotgun sequence.
ACCESSION   DAOPHV010000455 DAOPHV010000000
VERSION     DAOPHV010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67188_length_2273_cov_4.051849"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /locus_tag="PLQ29_10770"
     CDS             <1..1687
                     /locus_tag="PLQ29_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87171.1"
                     /translation="GARGTGARATSEDAALIKILEDGSTLPLVESSHGYWSPKVAFIA
                     TGSAETDDDGSIYICFDQMASTIDGSVQFIRVYPDNHYDIIWPIDPANFAYDSTGQVS
                     TWSWWGMDSEPLAKGPDGRLYFKVSRFNMSANADDIYAYDPRRPTEAPIRITPENSTF
                     SIENFVVDAKGHLFIQGRPGGDYEASFMRYYTDGVTSPNIIYYASTSGSTWVRGYTTD
                     PSGNYLIMNGYNIRGMNGIIKATIVDATTVEYQLLYPNSCGMNTWIGLTKYYGDGWTS
                     PTAVIKQTTQGWPTSYDWLDEVTTSGVFDGAKFTARIASYFKPGETLAFPTTTPDWWK
                     DDPDKPIGTTGSSVTIGTSPITVAVDGGTTLGSLIQQYPEEVLRELYPANKLFKDWLA
                     ENPGFENINFDNIGAMAWASDGLYGLYSSAWWGSSSSDAKLVKLVDRNGDPDLRVVQL
                     GHSAEYPSQIKIQGDYIYYRYAILDGANQETGKHSIARQNFVGGAQEELLAPLGKDIE
                     INNYDVSGDDSILYFTGYDIAANAVVGGRIELDTKVYIPMESAVELSQIRVID"
     gene            1744..>2273
                     /locus_tag="PLQ29_10775"
     CDS             1744..>2273
                     /locus_tag="PLQ29_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87172.1"
                     /translation="MAFKRITIAALIALTITAAFADGSEPAPGDETALTANISRFVQL
                     DLMAAAGMMAADEGVAAMRAYSAGLDPFYREILYERYKKDATGAALLNFFTGGLGSWT
                     MGDRLAGGVLTAGGVAVMAMSALMMPMGLGLDIDVRAMSKAAMISGTVVGVAGIAYPY
                     ISAHRRNARLREALSY"
BASE COUNT          458 a          766 c          680 g          369 t
ORIGIN      
        1 gggcgcccgg ggaaccggcg cgagggccac ctccgaggac gccgccctca tcaagatact
       61 cgaggacggg tcgaccctgc cgctcgtcga gtcgagccac ggctattgga gccccaaggt
      121 ggccttcatc gccaccggct ccgccgagac cgacgacgat ggctccatct atatctgctt
      181 cgaccagatg gccagcacga tagacggctc ggtccagttc atccgcgtct accccgataa
      241 ccattacgac atcatctggc cgatcgaccc ggcgaatttc gcctacgact cgacgggcca
      301 ggtgtccacc tggagctggt ggggcatgga ctccgagccc ctggccaagg gccccgacgg
      361 ccgcctctac ttcaaggtct cgcgcttcaa catgagcgcg aacgccgacg acatctatgc
      421 ctacgacccg cgccgaccga ccgaggcgcc catccgcatc acgcctgaga attcgacgtt
      481 ctccatcgaa aacttcgtgg tcgacgccaa ggggcacctg ttcatccagg gccggccggg
      541 cggcgactac gaggcctcgt tcatgcgtta ttacacggac ggcgtcacat cgccgaatat
      601 catctactac gcctcgacga gcggtagcac ctgggtccgc ggctatacca ccgacccgag
      661 cggcaactac ctgatcatga acggctataa catccgcggc atgaacggca tcatcaaggc
      721 caccatcgtc gacgctacca ccgtcgagta ccagctcctg tacccgaact cctgtggcat
      781 gaacacctgg atcggcctaa ccaagtacta cggtgacggc tggaccagcc ccacggcggt
      841 catcaagcag acgacgcagg gctggccgac cagctatgac tggcttgacg aggtcacgac
      901 gagcggcgtg ttcgacgggg ccaagttcac cgctcggatc gcctcgtact tcaagcccgg
      961 agagacgctc gccttcccca ccaccacgcc cgattggtgg aaggacgatc ccgataagcc
     1021 gatcgggacc accgggtcat cggtaacgat cggcacctcg ccgattactg tcgcggtaga
     1081 cggaggcaca actctcggtt cgctcatcca gcagtatcct gaagaggtgc tcagggagct
     1141 ctaccccgcg aacaagctct tcaaggactg gctcgccgag aaccccggat tcgaaaatat
     1201 caacttcgac aacataggcg ccatggcctg ggcctcggat gggctctacg gcctctactc
     1261 gagcgcctgg tggggcagct cctcgtcgga cgccaagctc gtcaagctgg tcgacaggaa
     1321 cggagatccc gacctgaggg tggtccagct cggtcatagc gccgagtacc cgagccagat
     1381 caagatccag ggcgactaca tctactaccg ctacgccatc ctcgacggcg cgaaccagga
     1441 gacaggcaag cacagcatcg ccaggcagaa cttcgtgggc ggggcccagg aagaattgct
     1501 cgcgcccttg ggcaaggata tcgagattaa taactacgac gtctcgggcg acgactcgat
     1561 cctctacttc accggctacg acatcgcggc gaacgcggtg gtcggcgggc gcatcgagct
     1621 ggacaccaag gtctacatcc cgatggaatc ggccgtcgag ctgtcccaga tcagggtcat
     1681 agactgacga gcgagctacc gagccgccgc cactgcgagg cggcggctcc cacggagaaa
     1741 accatggctt tcaagcgtat cacgatagcg gcgctcattg cccttaccat cacggccgcc
     1801 ttcgccgacg gctcggaacc ggcgccgggc gacgagacag ccctgaccgc caacatctcg
     1861 cgcttcgtgc agctggacct catggccgcg gccggcatga tggcagccga cgagggcgtc
     1921 gcggcgatgc gggcgtactc agccgggctg gaccccttct accgagagat actctacgag
     1981 cgctacaaga aggacgcgac cggcgccgcc ctgctcaact tctttacagg cgggctcggc
     2041 tcgtggacta tgggagacag gctggcgggc ggcgtgctaa ccgccggcgg cgtcgccgtc
     2101 atggccatgt cggcgctcat gatgcccatg gggctcggac tggacatcga cgtacgggcc
     2161 atgtcgaagg cggcgatgat aagcggcacc gtcgtcggcg tggccggtat cgcttacccc
     2221 tatatctccg cgcatcgccg caacgcgagg cttcgcgagg cgctgtcgta cta
//
LOCUS       DAOPHV010000456         2271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_67283_length_2271_cov_4.081227, whole genome shotgun sequence.
ACCESSION   DAOPHV010000456 DAOPHV010000000
VERSION     DAOPHV010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67283_length_2271_cov_4.081227"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1584)
                     /locus_tag="PLQ29_10780"
     CDS             complement(151..1584)
                     /locus_tag="PLQ29_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPG87173.1"
                     /translation="MEAGYQLFKDDETPLYIQATRAVRARILDGELRLGDRLPSVRGL
                     SDELGVNPATVVAAYRILTREGFIVAKAGSGAFVAPDAPVDGAVGEPSAAFPASERPL
                     ADGSSGAVDLAANAPPLDMFPLVDMKRFIVEAIDADGGKAFEYQDTGGYRPLRVAMAE
                     RLAARGRPGSVDPFDVHIVSGAQQGIDLAARILLRPGDVAAVENPGYRGARDAFVAAG
                     ARVEPVPVDSGGLDLDALEALARSRPLRLVHVNPGFQNPTCVVYTAERRSALASMAER
                     YGFYVLEDDQLAELSWDGSMPPAVRDFDEAGRVILVKSTSKCLMPGLRIAFLEAPTAL
                     RSRFEAVKRSIDISSNGLMQRALERFIASGRFDEHIAAARDRYRAAYGAFDAALAPCR
                     SLGLSWAPPGGGLNLWLALPSGVSARSFASSCLAAGCVLAPEASFRHEAAEPERADSH
                     LRVSFGSAPLESLSSAAMAMAAVARRL"
     gene            complement(1592..>2271)
                     /locus_tag="PLQ29_10785"
     CDS             complement(1592..>2271)
                     /locus_tag="PLQ29_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPG87174.1"
                     /translation="QKVLAIGNPFGLERTLTRGIVSGLGRPVQQDSKTIIRDMIQTDA
                     SINPGNSGGPLLNSRGELIGVNTMIYSPSGGSVGVGFAVPVDTAKRIVPDLIKYGMVK
                     RGWIDAEYVQLFPALIEYMKEKGDPLPIEKGLLVSSTKRAGNADRSGLRGGSTPVRYY
                     QSVFNVGGDVIVAVDGMAVSSLADLYSALEDNKPGDAIRVEYYRGSKRLAATITLSDR
                     AEYLGGK"
BASE COUNT          360 a          754 c          820 g          337 t
ORIGIN      
        1 ggaatcggtc tccggcgttg acgccggcgg ccagggcggc tatggcttcc ggctgatccc
       61 cggcgggctc gaatggggcg acgactttga acggtttcac gctagccagt atagctcctg
      121 ccagcgcgcg agaaaagcta taaaacgatt ctataaccgc ctggccaccg ccgccatcgc
      181 catggccgcg ctggacagcg attcgagcgg cgctgagccg aaactgactc gaaggtggct
      241 gtccgcccgc tcgggctccg cggcctcatg cctgaacgac gcctcgggcg caaggacgca
      301 gcccgccgcc agacaggacg aggcgaagga tcgcgctgag actccgctcg ggagggccag
      361 ccaaaggttc agccccccgc ccggaggcgc ccacgacagg ccgagcgatc ggcacggcgc
      421 gagagccgcg tcgaaggcgc cgtaggcggc gcggtaccgg tccctcgcgg cggctatgtg
      481 ctcgtcgaag cggccgcttg cgatgaagcg ctccagggcc cgctgcatga ggccgttcga
      541 cgatatatcg atggagcgct tgaccgcctc gaagcgggag cgaagggcgg tcggcgcctc
      601 gaggaaggcg atgcgcaggc cgggcatcag gcatttcgag gtgctcttga cgaggatgac
      661 cctgccggcc tcgtcgaagt cgcgcacggc cgggggcatc gacccgtccc acgatagctc
      721 ggcgagctgg tcgtcctcga gcacgtagaa gccgtagcgc tcggccatgg aagccagggc
      781 cgagcgccgc tcggccgtat agaccacgca ggtcgggttc tggaagccgg gattgacgtg
      841 gacgaggcgc aggggccggc tcctggccag ggcctcgagc gcgtccaggt caaggcctcc
      901 cgagtcgacg ggcaccggtt cgaccctcgc tccggcggcc acgaaggcgt ctcgggcgcc
      961 ccggtagccg gggttttcca cggcggccac gtcgccgggg cgcagcagga tacgggccgc
     1021 caggtcgatc ccctgctggg cgcccgagac gatatgcacg tcgaaggggt cgaccgagcc
     1081 cgggcgcccc cgggcggcta ggcgctcggc catggcgacg cgtagcgggc gatagccgcc
     1141 cgtgtcctgg tactcgaagg ccttgccacc gtcggcgtcg atggcctcga cgatgaaacg
     1201 cttcatatcg acgagcggga acatatcgag gggaggcgcg ttggccgcca ggtctaccgc
     1261 gccgctcgac ccgtcggcca gaggccgctc ggacgcagga aaggcggccg acggctcgcc
     1321 gacggccccg tcgaccggcg cgtccggggc gacaaacgct cccgagccgg ccttggcgac
     1381 gatgaagccc tcgcgggtca ggatgcggta ggcggcgacc acggtggccg gattgacgcc
     1441 gagctcgtcg gagagtccgc gcaccgaggg caggcggtcg ccgaggcgga gctcgccgtc
     1501 caggatgcgg gcgcgcacgg cgcgggtggc ctggatatag agcggcgtct cgtcgtcctt
     1561 gaacagctgg tagccggcct ccatcgggcg attacttgcc gcccaggtac tcggcccggt
     1621 cggacagcgt gatcgtcgcg gccagtcgct tggagccgcg gtagtactct acccgtatcg
     1681 cgtcgccggg cttgttgtcc tcgagcgcgg agtagaggtc agccagcgag ctgaccgcca
     1741 tgccgtccac ggcgacgatc acgtcgccgc cgacgttgaa cacgctctgg tagtagcgca
     1801 ccggcgtcga gccgccgcgc aggccggagc ggtcggcgtt gccggcccgt ttggtggacg
     1861 atacgagcaa gcccttctcg atcggaaggg ggtcgccctt ctccttcatg tactcgatca
     1921 gcgcggggaa cagctgcacg tactcggcgt cgatccaacc tcgcttgacc atgccgtact
     1981 tgatcaggtc gggcacgatg cgcttcgccg tgtcgaccgg caccgcgaag ccgaccccga
     2041 ccgaaccgcc cgacggggag tatatcatcg tgttgacgcc tatcagctcg ccgcgggagt
     2101 tgaggagcgg ccctccggaa ttgcccgggt tgatcgacgc gtcggtctgg atcatgtcgc
     2161 ggatgatggt cttggagtcc tgttgtaccg ggcgcccgag gcccgagacg atgccacggg
     2221 tcagggtgcg ctcgaggccg aagggattgc ctatcgcgag caccttctgg c
//
LOCUS       DAOPHV010000457         2271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_67284_length_2271_cov_3.950812, whole genome shotgun sequence.
ACCESSION   DAOPHV010000457 DAOPHV010000000
VERSION     DAOPHV010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67284_length_2271_cov_3.950812"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /gene="hisD"
                     /locus_tag="PLQ29_10790"
     CDS             <1..1143
                     /gene="hisD"
                     /locus_tag="PLQ29_10790"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPG87175.1"
                     /translation="ALARLAAELDGNAGRLWYGPDDFASALQSIPAAERGVLERAAGR
                     VGAFAAAQLASLGTVDLAVPGGRAGHEVLPVERAGCYVPGGRYPLPSTVLMTALVART
                     AGVADVAVACPRPSPLILAACAVAGADSLLAAGGAQAIAAFAYGAGPLPPRDVVVGPG
                     NRYVAAAKRLAQALVRTDAPAGPSELLVIADESADPTTVAWDLLAQAEHDPDAVPALA
                     ASSETVAGAVELALAACLGELGALRPESASIARAALANGWAFVSADRTELAAVADRFA
                     PEHLELAVVEARRLAGSCRHAGAIFMGGSSAEAFGDYGAGPNHTLPTSGRARSSGGLS
                     VFDFVRIRSWLAVDDADELLIADTALLAGAEGLFAHARSALRRSAP"
     gene            1241..1750
                     /locus_tag="PLQ29_10795"
     CDS             1241..1750
                     /locus_tag="PLQ29_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87176.1"
                     /translation="MKLFRAIATIMVMSLVAIPAVAQGSSEAGKADPRVAQALDSLGL
                     KYSVNSSNNYKVVYSMEDGSGRSHLAFVVSKTSKYRSLEIREIWSIAAILDEYPDAEV
                     VDYMFTMNSTTKIGAWAIEVSDDGEVWVMYTVKVPADLSADELNDIIYFVAEVCDELE
                     VELTGDDEY"
     gene            complement(2054..>2271)
                     /gene="ssrA"
                     /locus_tag="PLQ29_10800"
     tmRNA           complement(2054..>2271)
                     /gene="ssrA"
                     /locus_tag="PLQ29_10800"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          325 a          793 c          770 g          383 t
ORIGIN      
        1 gcgctcgccc gcctggcagc cgagctcgac ggcaacgccg gccggctgtg gtacggcccg
       61 gacgacttcg cctcggcgct gcaatcgatc ccggccgccg agcgcggggt cctggaacgg
      121 gcggccggca gggtaggggc cttcgccgcc gcccaactcg cctcgctcgg aaccgtggac
      181 ctcgcggtac ccggcggccg ggccggccac gaggtcctgc cggtggagcg ggccggctgc
      241 tacgtgcccg gaggccgcta tcccttaccg tccaccgtgc tgatgaccgc cctcgtcgcg
      301 aggaccgccg gcgtcgcgga cgtcgcggtc gcctgcccga ggccgtctcc cttgatactg
      361 gccgcctgcg ccgtcgcggg cgcggattcc ttgctcgcgg cgggcggcgc gcaggccata
      421 gccgccttcg cgtacggagc agggccattg ccgccccgcg acgtcgtcgt cggcccgggc
      481 aaccgatacg tcgccgccgc caagcgccta gcccaggcac tagtccgtac cgacgctccg
      541 gccggcccga gcgagctgct cgtcatcgcc gacgaatcgg ccgacccgac gacggtcgcc
      601 tgggatctgc tcgcccaggc ggagcacgat ccggacgccg tgccggccct ggccgcctcg
      661 tccgaaacgg tcgcgggcgc ggtggagctg gccctggcgg cctgcctcgg cgagcttgga
      721 gccctcaggc ccgagtcggc ctcgatagcc cgggcggccc tcgccaacgg gtgggccttc
      781 gtatcggccg accgtaccga gctagcggcg gtcgccgacc gcttcgcccc cgagcatctg
      841 gagctggccg tcgtcgaagc tcggcgcctg gccggctcgt gccgtcatgc cggggcgatc
      901 ttcatgggcg gttcgagcgc cgaagctttc ggcgactacg gcgcgggccc gaaccacacc
      961 ctgcccacct ccgggcgggc tcgatcgtcg ggcggcctgt cggtgttcga cttcgtgcgc
     1021 atacgcagtt ggctggcagt cgacgacgcc gacgagcttc tcatcgccga cacggcgcta
     1081 ctggccgggg ccgagggcct gttcgctcat gcgagatcgg ccctacggcg ctccgctccg
     1141 tgagtggtac gaagattgtg gaccctgtaa ttcaagcgcc gacggactat actagcgata
     1201 cgcgcttata tcgattctac cgataggaag ggagcaatct atgaaactgt tcagggctat
     1261 cgcgacgatc atggtcatgt cgctcgtcgc cattcccgcc gtcgcccaag gtagctcgga
     1321 ggcgggcaag gccgatcctc gggtggccca ggcgctcgac tccttgggcc tcaagtactc
     1381 ggtcaactcg tcgaataact acaaagtcgt ctacagcatg gaagacggct ccggccgctc
     1441 gcacctggcc ttcgtcgtct ccaagacctc caagtaccgg agccttgaaa tacgggagat
     1501 atggtcgatc gcggccatcc tggacgagta tccggacgcc gaggtcgtcg attacatgtt
     1561 cacgatgaac tcgacgacca agatcggcgc ttgggccatc gaggtgtcgg acgacggcga
     1621 ggtctgggtc atgtatacgg tcaaggtgcc cgccgacctg tcagcggacg agctcaacga
     1681 tatcatctac ttcgtcgccg aggtctgcga cgagctcgag gtcgagttga ctggcgacga
     1741 cgagtactga tcccgtacct tctcacgggt tacccgctcg cgtacaacga gaagccccgc
     1801 cttgcggcgg ggcttctcgt gtttggaggt ggcgagagtc ggcttccagt cgcgcgcgtc
     1861 ctgcgcgctc cttccagccc gcctacgggc cggtgctcgc tcctgctcgc acatcggcag
     1921 gggcgcaagg gggatacccc ctcgagcgac ggcgagccgc cgctctcacc ccagcgccgc
     1981 tgcgagtctc gcgtcgcatg gttcgactcc cccgaaaaca agtcaggctc cgctttcgcg
     2041 gagcctgact tgtttttgga ggtgggggga gtcgaacccc cgtcctatca cgcggaagga
     2101 aggcttctac aggtttaggc ggcctttcaa gttgtcggga ggcgggcggc gggccgccgc
     2161 gctatcgcct ccgtatcccg cttggtgtcc cccccgtccc gcgggcgtgg cggggggtaa
     2221 gccctggtcg ggttacagac cggtcgcggc gctcagagcg gggcgccgcg g
//
LOCUS       DAOPHV010000458         2270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_67319_length_2270_cov_3.849661, whole genome shotgun sequence.
ACCESSION   DAOPHV010000458 DAOPHV010000000
VERSION     DAOPHV010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67319_length_2270_cov_3.849661"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /gene="gcvPB"
                     /locus_tag="PLQ29_10805"
     CDS             <1..1102
                     /gene="gcvPB"
                     /locus_tag="PLQ29_10805"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPG87177.1"
                     /translation="IWKLQESLKAIGGFEAVSLQPAAGAHGELAGVFMIRAYHLARGD
                     AKRVKMLIPDSAHGTNPASCTMAGLTTVTIPSDAEGGVDMAALESACDDTVAGIMITN
                     PSTLGLFERNIEKIARIVHAAGGLVYGDGANMNALTGVLRPADVGIDVMHFNLHKTFS
                     TPHGGGGPGVGMVGAGKALAAYLPGPIAVREGDGYAMAAPSKTIGRMKAFYGNFGVLV
                     RAYTYIRMLGGDGLRRVAESAVLNANYLKARLEKAYPVKYPRRCMHEFVAMGNFAEGV
                     HTLDIAKRMIDYGFHPPTIYFPLIVPEALMIEPTETESKETLDGFVEVMLKIAEEAKS
                     SPELLREAPHATPVGRLDEVAAARCPVLCYRG"
     gene            1185..>2270
                     /locus_tag="PLQ29_10810"
     CDS             1185..>2270
                     /locus_tag="PLQ29_10810"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPG87178.1"
                     /translation="MIDVFAQQCLSRLSIVAALVFAYCAWMVYSLNREAKPNRIAVVF
                     NVLFAAWAAAASFWYGADDPSEALALYRAFSWTWSLFPPLILHFSLSVTGNPVLDGRF
                     RRPFQVALYAPGALLCFLTPAYVVTEPVYRGGYWMLAVDTNAAYAFFVIHYLAMVVAS
                     GWIIFAARRRAEDRRTAKRLTVLGRSYVAASALGFTTDTAFLMLGVDFPNMAILWILI
                     LSLGMIYSMKRYGLLSILPPDEAVSVLESLGEFVLYVDEGARLVWANPSAIAAIGATG
                     LNRARGMRCREFLRGDVERWLADPAAFRLEPRGVSATLGPDSIPVSLWIHPVGEASSE
                     GAVLTAVDLRAEFANARTKRRLADADRL"
BASE COUNT          361 a          796 c          716 g          397 t
ORIGIN      
        1 catctggaag ctccaggaga gcctcaaggc catcggcggc ttcgaggccg tcagcctgca
       61 accggccgct ggcgcccacg gcgagctggc cggggtcttc atgatcaggg cctaccacct
      121 ggcccggggc gacgccaagc gcgtgaagat gctgataccc gacagcgccc acggcaccaa
      181 cccggcgtcg tgcacgatgg cgggcctgac cacggtcacc atcccgagcg acgccgaggg
      241 cggcgtcgac atggcggccc tcgagtccgc ctgcgacgac acggtcgccg gcatcatgat
      301 caccaatccg agcaccctcg gactcttcga gcgcaacatc gagaagatag ccaggatagt
      361 ccatgcggcc ggcggtctcg tctacggcga cggcgccaac atgaacgcgc tgaccggcgt
      421 gctcagaccg gccgacgtcg gcatcgacgt gatgcacttc aacctgcaca agaccttctc
      481 cacgcctcat ggcggcggcg gccccggcgt cggcatggtc ggggccggaa aggccctcgc
      541 cgcgtacctg cccgggccga tagccgtccg cgagggcgac ggctacgcga tggcggctcc
      601 atccaagacc ataggccgca tgaaggcttt ctatggcaac ttcggcgtgt tggtccgagc
      661 ctatacctac atcaggatgc tcggtggcga tggcctgcgg cgcgtggccg agtctgcggt
      721 gctgaacgcg aactacctca aggccaggct cgagaaggcc tacccggtca agtacccacg
      781 ccgctgcatg cacgagttcg tggcgatggg caacttcgcc gagggcgtcc ataccctgga
      841 catcgccaag cgtatgatag actacggctt ccatccgccg acaatctatt tcccgctcat
      901 cgtcccggag gccctgatga tagagccgac cgagaccgag agcaaggaaa cgctcgacgg
      961 cttcgtcgag gtgatgctca agatagccga ggaggcgaag agctctccgg agctgctccg
     1021 cgaggcgccc catgctacgc cggtgggccg actcgacgag gtggccgcgg ccaggtgtcc
     1081 ggtgctctgc taccggggct gagcgaggta tcgtcgtatc gtcgggcggg gaggccaagc
     1141 ctccccgcct tttctttcat cccggcgtag tatagaatcc gcccatgatc gacgttttcg
     1201 cgcagcagtg cttgtccagg ctatcgatcg tcgcggcgct cgtgttcgcg tattgcgcct
     1261 ggatggtgta ctcgctgaac cgggaagcta agccgaaccg gatcgcggtc gtcttcaatg
     1321 tcctgttcgc ggcctgggcc gccgccgcct cgttctggta cggcgccgac gacccgtccg
     1381 aagcgttggc gctataccga gccttctcgt ggacctggtc gctcttcccg ccgctcatcc
     1441 tgcatttctc cctcagcgtt accggcaatc ccgttctcga cggccgcttc cgccggccgt
     1501 tccaggtcgc cctctacgcg ccgggcgcgc tcctgtgctt cctgaccccg gcctatgtcg
     1561 tgaccgagcc ggtctatcgc ggcggctact ggatgctggc cgtcgacacc aacgccgcct
     1621 acgccttctt cgtcatccat tacctggcca tggtcgtcgc ttccgggtgg atcatcttcg
     1681 ccgcgaggcg tcgggccgag gatcggcgca ccgccaagcg gctgaccgtg ctcgggcgat
     1741 cctacgtcgc cgccagcgcc ctcggcttca ccaccgacac ggcgttcctg atgctcggcg
     1801 tcgattttcc gaacatggcc atcctgtgga tcctgatcct gtccctggga atgatctact
     1861 cgatgaagcg ctacggcctc ctatcgatac tgccgccgga cgaggccgtc tcggtgctcg
     1921 agagcctcgg cgagttcgtc ctctacgtcg acgaaggggc ccgcctggta tgggccaatc
     1981 cgagcgccat agccgccatc ggggcgaccg gcctcaaccg ggccagggga atgcgctgtc
     2041 gagagtttct gcgaggcgac gtcgagcgct ggctcgccga ccccgccgcc ttcaggctcg
     2101 agccgcgcgg cgtctcggcg acgctcggac ccgattccat acctgtcagc ctgtggatcc
     2161 atccggtcgg cgaggccagc tccgagggcg ccgtactgac cgcggtcgac ctgcgagccg
     2221 agttcgccaa cgcgcgcacc aagcgcaggc tcgccgacgc cgaccgactg
//
LOCUS       DAOPHV010000459         2262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_67624_length_2262_cov_3.816040, whole genome shotgun sequence.
ACCESSION   DAOPHV010000459 DAOPHV010000000
VERSION     DAOPHV010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67624_length_2262_cov_3.816040"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="PLQ29_10815"
     CDS             complement(<1..1237)
                     /locus_tag="PLQ29_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPG87179.1"
                     /translation="MALANAASVSFTINAAPSSGGTLSMSGVQTKKVGESFSITATVF
                     DAYVFGGWSSSGGGEVAFTYPNASEKTTTVATIGAASDDIVITATFVARPYVKTITPS
                     EGLQVVRNQPIIANFSKPMDPASFGIPGNIIVTYDAIDPMTANPKTILASGTNSDYLS
                     IEVSATRLRLIPKSGSPIAGGYLPASSTITVFLGKDVSDSEGNAMAESKEWYFYTNNS
                     TDISPPEIDGITIRNGNSSAAEALSFTSGSTYHAVKSSTVTVEIAAHTTSASSSVQSM
                     VLIESGNSGGIPSSRTTELGYADSYTYTLATSDDGPKTLTYYVRSSTGIESAHESVNI
                     YLDTTPPTLNLGAISSNNGTNSAFAVNGNTVSLGFSAADPSGSGLYSTAATIAGQTAD
                     VPSISGAEKRPEPERSAALN"
     gene            complement(1363..>2262)
                     /locus_tag="PLQ29_10820"
     CDS             complement(1363..>2262)
                     /locus_tag="PLQ29_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87180.1"
                     /translation="PAAAAAEDASGLAQEGGPTPDPTPSQRPVVDLDHTSAPEAGLAE
                     RPAAPAEADGKPIRSAYVPAARFAPTVETHAAPAFGKRLPDGADASGYERLPVSAEPP
                     RARTIDLRAPEIRVGSGTWKREESRAAMTDPKAYYAKLVIPTPGLSGTTAYEFKARAT
                     GSGWVGFGLHVHGRGAWRLTGYGGGDSLLVWVTSDPKAYGDAAPRLQVYRSRGEVAMD
                     LLAEARLVGSAFEDAAYRVEYDPAAGSVRAYVNGSLALSASGIANPAPFEYAALRALD
                     TAEFSDLTIQDASTGAAPTEKTP"
BASE COUNT          364 a          667 c          779 g          452 t
ORIGIN      
        1 gattcagcgc ggccgacctc tcgggttcgg gcctcttctc ggctccggaa atactcggaa
       61 cgtcggcggt ctgcccggct atggtggccg cggtcgagta gaggcccgaa cccgaggggt
      121 cggccgcgct gaatcccagg ctcacggtgt tgccatttac cgcgaacgcg ctattggtgc
      181 cgttgttcga gctgatcgct ccgaggttca gggtcggagg cgtcgtatcc aagtagatat
      241 tgacggactc atgggccgac tcgatccctg tcgagctcct gacgtagtac gtcaaagtct
      301 tcggtccgtc atcgcttgtc gctagcgtgt aggtgtagct gtcggcatag cctagctcgg
      361 tcgtcctgga gcttgggatc ccaccagaat tacctgattc gatcaacacc atgctctgga
      421 ccgaagacga tgcggaagtg gtatgggcgg ctatctccac cgtaactgtc gacgacttga
      481 ccgcatgata cgtggagccg ctcgtaaagc tcagggcctc ggcagccgag ctgtttccat
      541 tccgtatcgt tataccgtct atttctggcg gagaaatgtc tgttgagttg tttgtataga
      601 aataccattc cttgctttcg gccatggcat tgccttcaga gtctgataca tccttgccta
      661 aaaaaaccgt gatagtgcta ctcgctggga ggtatccgcc cgcgattggc gaaccactct
      721 tgggtatcag ccgtaaacgc gtggcgctaa cctctataga gagatagtct gagtttgttc
      781 ccgatgctag tatcgtcttg ggattggctg tcatcgggtc gatcgcatcg tatgtaacga
      841 ttatgtttcc aggtattcca aaagaagccg gatccatggg tttgctaaaa ttcgcgatga
      901 taggctggtt cctgaccacc tgcaaacctt cagatggcgt gatggtcttg acgtagggcc
      961 tggcgacgaa ggtagcggtg atgacgatgt cgtcggaggc ggcgcctatc gtcgccacgg
     1021 tcgtcgtctt ctcgctcgcg ttgggatatg tgaacgcaac ctcgccgcca ccggacgatg
     1081 accatccgcc gaagacgtac gcatcgaaaa ccgtcgccgt gatactgaag ctctccccga
     1141 cctttttggt ctgcacgccg ctcatcgaca gggtgccgcc cgacgacggc gcggcgttga
     1201 tcgtgaagct aacgctcgcc gcgttcgcca gcgccacgtc ctgttggatc tgctccttga
     1261 ggttctcggt gccgaagaag tcgtcgcagg tggacagggc ggcgatcgag gcggctgcta
     1321 cgatgacgag gccgatacgg cgggcgagcg ggcggttggg gttcatgggg tcttctccgt
     1381 gggggcggcg ccggtcgagg cgtcctggat ggtcaggtca gagaactcgg cggtatcgag
     1441 ggctcgcagg gcggcgtact cgaagggggc gggattggcg atgccggagg ccgagagggc
     1501 gagggatcca ttcacgtagg cgcgtacgga gccggccgcc gggtcgtatt cgacgcggta
     1561 ggcggcgtcc tcgaaggcgg agccgaccag cctcgcctcg gcgagcaggt ccatggccac
     1621 ctcgccgcga gaccggtaga cctggaggcg gggcgcggcg tcgccgtagg ccttggggtc
     1681 gctcgtcacc cagacgagca gggagtcgcc gccgccgtaa ccggtcagcc tccaggctcc
     1741 gcgcccgtgg acgtggaggc cgaagccgac ccagccggaa ccggtggcgc gcgccttgaa
     1801 ctcataggcg gtcgtaccgg acagtccggg cgtcgggatg accagcttgg cgtagtaggc
     1861 cttggggtcg gtcatcgcgg cgcggctctc ctcgcgcttc caggtgcctg agccgacgcg
     1921 aatctccggc gctcgcaggt ctatggtccg agcgcggggc ggctcggcgg agacgggcag
     1981 gcgctcgtag ccggacgcgt ccgcgccgtc gggcagccgc ttcccgaagg cgggcgcggc
     2041 gtgcgtctcg acggtcgggg cgaagcgggc ggccgggacg taggccgacc gtatcggctt
     2101 tccgtcggcc tcggccggag ccgccggtcg ctcggccagc ccggcttccg gggcgcttgt
     2161 atggtcaagg tcgactactg gacgttgcga tggcgtagga tcgggtgtcg ggccgccttc
     2221 ctgagcgagc ccggacgcgt cctcggccgc tgccgcggcc gg
//
LOCUS       DAOPHV010000460         2260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_67704_length_2260_cov_3.594558, whole genome shotgun sequence.
ACCESSION   DAOPHV010000460 DAOPHV010000000
VERSION     DAOPHV010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67704_length_2260_cov_3.594558"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /gene="rplK"
                     /locus_tag="PLQ29_10825"
     CDS             <1..332
                     /gene="rplK"
                     /locus_tag="PLQ29_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPG87181.1"
                     /translation="SAPQFCQQFNDRTKSMEAGLTIPCVITVYKDKTFTFITKTPPAS
                     VLIKKAIGLDKGSAVPHKQKVGKIPHAKVTEIAKLKLKDLSANDVEAAERIIAGTARA
                     MGVEVEW"
     gene            336..1019
                     /gene="rplA"
                     /locus_tag="PLQ29_10830"
     CDS             336..1019
                     /gene="rplA"
                     /locus_tag="PLQ29_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPG87182.1"
                     /translation="MKHGKKYREVAKKVESDKAYELAEACVLLKELKVAKFDETVEAH
                     IRLNLKKSQTVRDTVVLPFQFKAEKKVLVFCKPDKEKEALEAGAAYVGSDELIEKIKG
                     GWMDFDIAVATPDMMKDVGKLGMVLGRRGLMPNPKTGTVTFDIKAAIGELKKGRTEFR
                     TDKTNIVHISVGKLSMEPAQVASNLETLLAEIQRKRPSDVKGEFVQSVYVAGTMTPSV
                     KVALAKVER"
     gene            1023..1556
                     /gene="rplJ"
                     /locus_tag="PLQ29_10835"
     CDS             1023..1556
                     /gene="rplJ"
                     /locus_tag="PLQ29_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPG87183.1"
                     /translation="MALRAKKTQPSKVEAIEAVGAKIAASSDFIFTDYRGLTVEQITA
                     LRAQLREKNAEYHIVKNNFARIAFEQKQFPDVSGLLVGPTAVAFAKKDSNEVAKIILE
                     FGKETSVKLKGGLVDKDVYDKVQMEAFSKLPGKSQLLSMLMSAMNAPLQNLVYALNGV
                     PTKLVRTLQAVADKKGA"
     gene            1701..2087
                     /gene="rplL"
                     /locus_tag="PLQ29_10840"
     CDS             1701..2087
                     /gene="rplL"
                     /locus_tag="PLQ29_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPG87184.1"
                     /translation="MAALTTDQILDAIAEMTVLEVSALVKAMEEKFGVTAAAPVAMVA
                     GAAGAAAAPAEEQTEFTVILKGNVPADKKIAIIKEVRTITGLGLKEAKDLVEAGDKPL
                     KEGIAKDEAEKIKKAITEAGGAVEIK"
BASE COUNT          498 a          713 c          705 g          344 t
ORIGIN      
        1 tcagcgcccc gcagttctgc cagcagttca acgacaggac caagtcgatg gaggcggggc
       61 tcaccattcc ctgcgtcatc acggtctaca aggacaagac cttcaccttc atcacgaaga
      121 cgccgcccgc gtccgtcctc atcaagaagg cgatcggcct tgataagggc tctgcggtgc
      181 cccacaagca gaaggtcggc aagatccccc atgccaaggt gacggagatc gccaagctca
      241 agctcaagga tctctcggcc aacgatgttg aggccgccga gcgcatcata gccggtaccg
      301 cccgcgccat gggcgtcgaa gtggagtggt aagcgatgaa gcacggtaag aaatacaggg
      361 aagtcgccaa gaaggtcgag tccgacaagg cctacgagct cgccgaggcc tgcgttctgc
      421 ttaaggagct caaggtcgcc aagttcgacg agacggtcga ggcccatatc aggctcaacc
      481 tcaagaagag ccagacggtc cgcgacacgg tcgtcctccc cttccagttc aaggcagaga
      541 agaaggtcct cgtcttctgc aagcccgaca aggagaagga agccctcgag gccggagccg
      601 cctatgtcgg tagcgacgag ctgatcgaga agatcaaggg cggctggatg gacttcgaca
      661 tcgccgtcgc gacgcccgac atgatgaagg acgtcggtaa gctcggtatg gtgctcggac
      721 gacgcggcct catgccgaac ccgaagaccg gtaccgtcac cttcgatatc aaggccgcca
      781 tcggcgagct caagaagggc cgtaccgagt tcaggaccga caagaccaac atcgtccata
      841 tctcggtcgg aaagctgtcg atggaaccgg ctcaggtcgc ctccaatctc gagacgctgc
      901 tcgccgagat ccagcgcaag cgcccgtccg acgtgaaggg cgaattcgtc cagtccgtgt
      961 acgtggccgg cacgatgacg ccgagcgtca aggtcgccct ggccaaggta gagaggtaag
     1021 acatggcctt acgagcgaag aagacccagc ctagcaaggt cgaggccatc gaggccgtcg
     1081 gcgccaagat cgccgcctcg agcgacttca tattcaccga ttatcgcggc ctgaccgtgg
     1141 agcagattac cgccctcagg gcgcagctcc gcgagaagaa cgccgagtac cacatcgtca
     1201 agaacaactt cgcgcgcatc gcgttcgagc agaagcagtt cccggacgta agtggcctgc
     1261 tcgtcggccc gaccgccgtg gccttcgcca agaaagactc gaacgaggtc gccaagatca
     1321 tcctcgagtt cggcaaggag acctcggtca agctcaaggg cggcctcgtc gacaaggatg
     1381 tgtacgacaa ggtccagatg gaggcgttct ccaagctacc gggcaagagt cagctcctgt
     1441 cgatgctcat gagcgcgatg aacgctccgc tccagaacct ggtctacgcc ctgaacgggg
     1501 ttccgacgaa gctcgtccgg accctgcagg ccgtggccga caagaagggc gcctaagcga
     1561 tcctgtacag agactcgcgg cgttcaggct tcggtggctg ccgtacctgt tcgccttgat
     1621 ccctccccgt ggggcgaccc gcggggaaga cgctcctccg taggaagagc tatcgttttt
     1681 agccataagg agaagataat atggcagcgc ttaccaccga tcagatcctg gatgccatcg
     1741 ccgagatgac cgtcctcgag gtttccgccc tcgtcaaggc catggaagag aagttcggcg
     1801 tcaccgccgc ggctcccgtc gcgatggtcg ccggcgccgc cggcgccgcg gccgccccgg
     1861 ccgaggagca gaccgagttc accgtgatcc tcaagggcaa cgtgcccgcc gacaagaaga
     1921 tcgcgatcat caaggaagtc cgcaccatca cgggcctggg cctcaaggag gccaaggatc
     1981 tcgtcgaggc gggcgacaag cccctgaagg agggcatcgc caaggacgag gccgagaaga
     2041 tcaagaaggc gatcaccgag gccggcggcg cggtcgagat aaagtaagga acttgaagaa
     2101 agcgtcgaat caacgctact cccgccgcgg catcgtgccg cggtcagagg taccgggcgc
     2161 cctggcgcgc ccggtacctt ttcgcgcctt ccacggctga gtcggcggac ggcgcccctg
     2221 gcgcggcaag tcgtcgcggc gcccccatgg gggcgacgcg
//
LOCUS       DAOPHV010000461         2249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_68204_length_2249_cov_2.805378, whole genome shotgun sequence.
ACCESSION   DAOPHV010000461 DAOPHV010000000
VERSION     DAOPHV010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68204_length_2249_cov_2.805378"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PLQ29_10845"
     CDS             <1..496
                     /locus_tag="PLQ29_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87185.1"
                     /translation="DKEYTLVGQLVEWREILTRKGKRMAFGKVEGYDGVLEIVVFSAT
                     LEANPGRFAQDAIVFLKGTVDLDRENPSFKVDRLVQPAELKEKSYRTMHIALHQVRRE
                     DDLEGLRDLLFGSSGQCGVVLHVRGGDDEVAVRAHAQISCSPNPDVVERIRETAIVSE
                     VWLD"
     gene            complement(493..1194)
                     /locus_tag="PLQ29_10850"
     CDS             complement(493..1194)
                     /locus_tag="PLQ29_10850"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPG87186.1"
                     /translation="MPIRAVGFDIDGTIYEASRLYAKLLVKGFGKIRFLLAFNAVRHE
                     FRDLLSSEEYRALGIRGVEAFHRYQAGLVARRLGRGADHIYEKIEDFFYTATVEPFAS
                     IPTYPGVADLLDELRAGGLKLGALSDFPCERKIGLLGLAGKFDVAMTSEETGLVKPDR
                     ASFDLLAERLGVRNDELLYIGNSERYDVLGALGAGMRAALITKDAKAAAATKAEFSFF
                     DYADLGRYLAERAAG"
     gene            complement(1286..>2249)
                     /locus_tag="PLQ29_10855"
     CDS             complement(1286..>2249)
                     /locus_tag="PLQ29_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPG87187.1"
                     /translation="VFDTQFSADLTIMEEGSELLRRIGERGTLPMITSCSPGWISFIE
                     TFYPALLPHVSTCKSPQQMFGSIAKTFWARKAGVDPSKLVVVSIMPCTAKKYEAKRPE
                     MRSAWEWWKDHGGKDSPYFDVDYALTTRELARMVRRAGIDFEALPDESFDDPLGASTG
                     AATIFGSTGGVMEAALRTAYELAVGEPLPSLDLEAVRGFDGVKEASLDVAGTQLRVAV
                     AHTLANARKLLDGIAAGTSPYTFVEIMTCPGGCLGGGGQPIAPTWEKRERRKEAIYAE
                     DRGMPLRKSHENESVKALYAEFLEKPLGHESHRLLHTKYAEKRA"
BASE COUNT          365 a          714 c          790 g          380 t
ORIGIN      
        1 cgataaggag tacaccctgg tcggccagct ggtcgagtgg cgggagatcc tgacgcgcaa
       61 aggcaagcgc atggccttcg gcaaggtcga gggctacgac ggcgtcctgg agatcgtcgt
      121 gttctcggct acgctcgagg cgaaccccgg ccgcttcgcc caggacgcga tcgtgttcct
      181 caagggcacc gtcgacctgg accgcgagaa cccgagcttc aaggtcgacc gcctcgtcca
      241 gccggccgag ctcaaggaga agagctaccg gaccatgcac atcgccctcc accaggtacg
      301 ccgggaggac gacctcgagg ggctgcgcga cctgctgttc ggctcgagcg ggcagtgcgg
      361 cgtcgtgctg cacgtccggg gcggggacga cgaggtggcg gtcagggcgc acgcgcagat
      421 cagctgctcg ccgaaccccg acgtggtgga gcggatccgg gagacggcga tcgtctccga
      481 ggtctggctc gactagccgg cggcgcgctc ggccaggtag cggcccaggt cggcgtagtc
      541 gaagaacgag aattcggcct tggtggccgc ggccgccttg gcgtccttgg tgatcagggc
      601 ggcgcgcatg cccgcgccga gcgcgccgag cacgtcgtag cgctcggaat tgccgatgta
      661 gagtagctcg tcgttgcgca cgccgagccg ctcggcgagc aagtcgaacg aggcgcggtc
      721 cggcttgacg agcccggtct cctcgctggt catcgccacg tcgaactttc cggccaggcc
      781 gaggagccct atcttgcgct cgcaggggaa gtcggacaga gcgcccagct tgaggccccc
      841 ggcccgcagc tcgtcgagga ggtccgccac gccggggtag gtcggtatcg aggcgaaggg
      901 ctcgacggta gccgtataga agaagtcctc tatcttctcg tagatatggt cggcaccgcg
      961 gccgaggcgc ctggcgacga ggccggcctg atagcggtgg aacgcttcca cgccgcgtat
     1021 gccgagggcg cggtactcct ccgacgatag gaggtcgcga aactcgtggc gcaccgcgtt
     1081 gaacgcgagg aggaagcgga tcttgccgaa tcccttgacg aggagcttgg cgtagagcct
     1141 ggaagcctcg tagatggtac cgtcgatgtc gaatccaact gctcgaatgg gcatggacac
     1201 gttatacagg gtatggccac tcctgtctat gtaatacggc cggccgcccg agatggacgg
     1261 ccggcccgga actagcggag cgggcttagg cccgcttctc cgcgtacttc gtatgcagga
     1321 gcctatgcga ctcgtggccg agcggcttct cgaggaactc ggcgtacaga gccttgacgg
     1381 actcgttctc gtgggacttt cgcagcggca tgccgcgatc ctcggcgtag atggcttctt
     1441 tacgccgttc acgcttttcc caggtcgggg cgatcggctg gccgccgcct ccgaggcagc
     1501 cgccggggca ggtcatgatc tcgacgaagg tgtaggggct ggtgccggcg gctatgccgt
     1561 cgaggagctt cctggcgttg gcgagggtat gcgccacggc cacccgtagc tgtgtgccgg
     1621 cgacgtcgag gctcgcctcc ttgacgccgt cgaagcctcg gaccgcctcg agatccaggc
     1681 tcggcagggg ctcgccgacg gccagctcgt acgcggtgcg cagcgcggcc tccatcacgc
     1741 cgccggtcga gccgaagatg gtcgcggccc cggtgctggc gccgagcgga tcgtcgaagc
     1801 tctcgtcggg cagggcctcg aagtcgatgc ccgcccggcg taccatgcgg gccagctccc
     1861 tcgtcgtcag ggcgtagtcg acgtcgaagt acggcgagtc cttccccccg tggtctttcc
     1921 accattccca ggcgctacgc atctccggcc gcttggcctc gtacttcttg gcggtgcatg
     1981 gcatgatcga cacgacgacc agcttggacg ggtcgacgcc ggctttccgg gcccagaagg
     2041 tcttggctat cgaaccgaac atttgctggg gcgacttgca ggtggagaca tggggcagga
     2101 gcgctgggta gaaggtttcg atgaagctga tccagcccgg cgagcacgag gtgatcatcg
     2161 gtagcgtccc acgctcgccg atacgcctga gcagctccga accttcctcc atgatggtca
     2221 ggtcggccga gaactgcgtg tcgaagacc
//
LOCUS       DAOPHV010000462         2248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_68218_length_2248_cov_5.072959, whole genome shotgun sequence.
ACCESSION   DAOPHV010000462 DAOPHV010000000
VERSION     DAOPHV010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68218_length_2248_cov_5.072959"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PLQ29_10860"
     CDS             <1..819
                     /locus_tag="PLQ29_10860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPG87188.1"
                     /translation="GFDVLSLANNHMNDYGPRAVDETLAYVDLLGIARCGAGRDLEEA
                     RRAAVLERDGVRFAFLAYAEGWWSVRGAESLRAAVAATRAEARLHGPEGLGVEATAHG
                     AGHSVGHGVAHALEEHILEDVARVKDVIRPDYVFVSLHWGDEHQRAPNAFQRGLGRAV
                     IDAGATAVLGHHPHVLQPIERYKHGLIAYSLGNFVFDMAAEATYETAALRLVLEGGRI
                     LRVEIEPLTIARGAYYPRAATPEEASARLRDLNRWSSRLSTPIVERAGKGVVDF"
     gene            932..>2248
                     /locus_tag="PLQ29_10865"
     CDS             932..>2248
                     /locus_tag="PLQ29_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87189.1"
                     /translation="MACDAGSALFTDEELDSMFEIRLSTDGEALTDGASLSSLVPIDI
                     SVSRLPGAPEASSISISLYDADGDEAASIAFSPSPVASGNAVRVDDLFGELDPFSIPS
                     GLPEGYYELRARVLDSRGQALSTYSTYALVYSGSLGPVGLEVYPGGVERGKVSLLRLT
                     GSLPSGQDPWIRWVVDGKVLAVGYLSERADRLAWRAPMVNGVYEAKVELFPFRPIAGS
                     TPPALAKAEIRLALSSAAPDPLADREAWSLFAFDGDLSDSGTRPRAVEPATIGSPYLE
                     THPLGYGYLLGDGLGLSSESAILPVDAPNGSLAAFSAAFTLGKADDGLAEGTGTLLSA
                     VLDSGQGLVIGVDTGYPYVAIGQALVRADSKLGSSLSRLAIDVSPAEGGAAIRFYIDD
                     TPVGESLLRSELFSSVPGACSVAGPGGFIAVYDELRVLRGAYPAFRL"
BASE COUNT          330 a          777 c          726 g          415 t
ORIGIN      
        1 ggcttcgacg tgctgtccct ggccaacaac catatgaacg actacgggcc gcgagcggtc
       61 gacgagacgc tcgcgtatgt cgacctgctc ggcatcgccc gctgtggcgc cggccgcgac
      121 ctcgaggagg ccaggcgcgc ggccgtgcta gagcgggacg gcgtccgttt cgccttcctg
      181 gcctacgcgg agggctggtg gagcgttcgg ggcgccgaaa gcctgcgtgc ggccgtcgcg
      241 gcgaccaggg ccgaggcgcg gctccacggc ccggaaggac tcggcgtcga ggcgaccgcc
      301 cacggagcgg gccactctgt gggccacgga gtggcccatg ccctcgagga gcacatcctc
      361 gaggacgtag cgcgcgtcaa ggacgtgata cgccccgact acgtgttcgt atcgctccac
      421 tggggcgacg agcaccagcg cgcgccgaac gcgttccagc gcgggctcgg ccgagctgtc
      481 atcgacgccg gcgccacggc ggtgctcggc catcaccccc atgtgctcca gccgatcgag
      541 cgttacaagc atggcctgat agcctactcg ctcggcaact tcgttttcga catggcggcc
      601 gaagcgacct acgagaccgc cgcactgcgc ctcgtgctcg agggcggcag gatcctgcgc
      661 gtcgagatcg agccgctgac catcgcgcga ggcgcttact atccccgcgc ggcgacgcca
      721 gaggaggcgt ccgcccgcct gcgggacctg aaccgatggt cgtcgcgcct ctccactcct
      781 atcgtcgaac gcgctggcaa aggcgtcgtc gatttctaac atttcttgtt tagaactgcc
      841 tttggtgcta tactcaagct tggataattg catgcgcgcg aatagggcgt tcctccgatt
      901 cctagtgcct ctgtctatct gcgtcgcggt gatggcgtgc gacgcgggct cggccttgtt
      961 caccgacgaa gagctcgatt cgatgttcga gatacgcctg tctaccgatg gcgaagccct
     1021 tactgacggc gcatcgctat cgtccctcgt gcctatcgat atctccgtct ccagactacc
     1081 cggcgctccc gaggcgtcgt cgatctccat ctcgctctac gacgccgacg gcgacgaggc
     1141 ggcctccatc gcgttctccc cgtctccggt cgcttcgggc aacgcggtac gggtcgacga
     1201 cctgttcggc gaactcgacc cgttttccat tccgtcgggc ctgcccgagg ggtactacga
     1261 gctacgcgcc cgggtactcg actcgcgcgg ccaggcgctg tcgacctatt ctacctacgc
     1321 cctcgtctat tccggatcgc tcgggccggt cgggctcgag gtgtatccag gcggcgtcga
     1381 gcgcggcaag gtgtcgctgc tacgcctgac cggatcgctc ccgtccggcc aggacccctg
     1441 gattcgatgg gtggtcgacg ggaaagtact agcggtcggc tacctatccg aacgggccga
     1501 caggctggct tggcgtgcgc ctatggtcaa cggcgtctac gaggcgaagg tcgagctctt
     1561 ccccttcagg cccatcgccg ggtcgacgcc tcccgcactc gccaaagccg agatcaggct
     1621 cgcgctctcc tcggccgcgc ccgatccact cgccgatcgc gaggcgtggt cgctattcgc
     1681 cttcgacggc gacctgtccg attctggcac gaggccacgg gcggtcgagc cggcaactat
     1741 cggttcaccg tatctcgaga cccatccgct cggatatggg tacctgctcg gcgacgggct
     1801 cggcctgtcg tccgagtcgg cgatactgcc cgtggacgcg cctaacggaa gcctcgcggc
     1861 gttctccgcc gcgttcacgc tcggcaaggc cgatgatggc ctggccgagg gtaccggcac
     1921 cctgctatcc gcagtcctgg actcgggcca gggcttggtc atcggcgtcg ataccggtta
     1981 tccatacgtc gcgatcggtc aggcgttggt acgggccgac tcgaagctag gctcgagcct
     2041 gagtcggctc gcgatagacg tatcgcctgc cgagggcggc gccgctatcc gcttctacat
     2101 cgacgatacg cccgttggcg aaagcctgct tcgaagcgag ctgttctcat ccgttcccgg
     2161 cgcctgctcc gtggccgggc cgggcgggtt catagccgta tacgatgagc tacgcgtgct
     2221 gcgcggcgcc tacccggcgt tccgcctg
//
LOCUS       DAOPHV010000463         2243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_68442_length_2243_cov_3.923218, whole genome shotgun sequence.
ACCESSION   DAOPHV010000463 DAOPHV010000000
VERSION     DAOPHV010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68442_length_2243_cov_3.923218"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PLQ29_10870"
     CDS             <1..973
                     /locus_tag="PLQ29_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HPG87190.1"
                     /translation="EVINGVEVLNTSGALEDLVIPVGSKDPARATEQVFLACNLDKRL
                     QEIPEGAAAETVRQGTWRVEEKVYDSFGTEHTLRVEFTRVTGQPNQWQAAVSIDPQAA
                     VATNPAVGLDPAALEGNVFIVEFDNLGTLRRLTDGQGNPSAEEGLLAMNVAFDVADTT
                     AGEGGEQVRQTFSLNLGVAGSVRNTVTQFAEASSTKVFEQDGYGMGYLENFKIDQSGM
                     ITAVYSNGSTRTLGQVALATFTNPNGLEKTGETNFVESNNSGMANIGPSGIAGKGKLI
                     AGALEMSNVDLAEQFTDMIVTQRGFQANSKTIQTSDQMLQELMTLKR"
     gene            1003..1200
                     /locus_tag="PLQ29_10875"
     CDS             1003..1200
                     /locus_tag="PLQ29_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HPG87191.1"
                     /translation="MIELTRLSGDGFWLNPHQIEYIERTPDTSIAMLSGKRVIVREGV
                     QDVLSKIVEYRRLIGAFKNEE"
     gene            1204..1986
                     /locus_tag="PLQ29_10880"
     CDS             1204..1986
                     /locus_tag="PLQ29_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HPG87192.1"
                     /translation="MDLATIIGLGAGTFCIIASMYASGGVVTTYMDAASVLMTIGGSF
                     FALMLNYSIKETLGIFKIMGKAFKVPDFGEMKIAESLLSLSERARREGILSLEEEIES
                     VDSAFMKRGLRMVVDSTDPEVIKNILETELSQMNERHGRWLKMIDQWAKLAPGMGMLG
                     TVQGLIAMMKNLEDKSRIGPNMAVALITTYYGAIMANFLFTPMMGKLANQDAAETKVR
                     EMVIEGVLSIQQGDNPHILQMKLSSYLSPESQKKLEELHPQS"
     gene            1999..>2243
                     /locus_tag="PLQ29_10885"
     CDS             1999..>2243
                     /locus_tag="PLQ29_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HPG87193.1"
                     /translation="MARKKKETALGGTGEWIVTYSDMVTLLLCFFVALFDTTEVDVVQ
                     LNQMISSLNNIGMGASTGGNTLSVGRMAELGNSINSLP"
BASE COUNT          469 a          711 c          693 g          370 t
ORIGIN      
        1 cgaggtgatc aacggcgtcg aggtgctcaa cacctccggc gccctcgagg acctggtcat
       61 cccggtcggc tccaaggacc cggcccgcgc caccgagcag gtcttcctgg cctgtaacct
      121 cgataagcgc ctccaggaga tccccgaggg agcggccgcc gagaccgtac gccagggtac
      181 ctggcgcgtc gaggagaagg tctacgactc cttcggtacc gagcatacgc ttcgcgtcga
      241 gttcacgcgg gtcacaggcc agccgaacca gtggcaggcg gccgtgtcca tcgaccccca
      301 ggccgcggta gccacgaacc cggcggtcgg cctggacccg gccgctctcg aggggaacgt
      361 cttcatagtc gagttcgaca acctgggcac actacgcagg ctgaccgacg gtcagggcaa
      421 cccctccgcc gaggaaggcc tcctggccat gaacgtggcc ttcgacgtgg ccgacaccac
      481 cgcgggcgag ggcggcgagc aggtcagaca gaccttctcg ctcaacctcg gcgtggccgg
      541 cagcgtcagg aacaccgtca cccagttcgc cgaggcatcg agcaccaagg tgttcgagca
      601 ggacggctac ggcatgggct acctcgagaa tttcaagatc gaccagtccg gtatgatcac
      661 ggccgtgtac tcgaacggct cgacgaggac cctcggccag gtcgcgctcg cgaccttcac
      721 caacccgaac ggcctcgaga agaccggcga gaccaacttc gtcgagtcga acaattccgg
      781 catggccaac atcgggccct ccggcatagc gggcaagggc aagctgatag ccggcgctct
      841 cgagatgtcc aacgtcgacc tggccgagca gttcaccgac atgatcgtga cgcagcgcgg
      901 cttccaggcc aactcgaaga ccatacagac ctcggatcag atgctccagg agctgatgac
      961 cctgaagcgg taatccaggc gtgacggcct gcggaggtgg cgatgatcga gctgactagg
     1021 ctttccgggg acgggttctg gctgaacccg catcagatcg agtacattga gcgcacgccc
     1081 gatacatcga tcgcgatgct gtccggcaag cgcgtcatcg tccgcgaggg cgttcaggac
     1141 gtactgagca agatagtgga ataccgccgc ctgatcggcg cgttcaagaa cgaggagtag
     1201 gggatggatc tcgctactat catcggcctc ggcgccggta ccttctgtat catcgcctcc
     1261 atgtacgcgt cgggcggcgt cgtcacgacc tatatggacg cggcgtctgt actgatgacg
     1321 ataggcggct cgttcttcgc gctgatgctc aactacagca tcaaggagac gctcggcatc
     1381 ttcaagatca tgggcaaggc cttcaaggta ccggactttg gcgagatgaa gatagccgag
     1441 tcgttgctct ccctgtccga gcgtgcccgc cgcgagggca tcctgtccct cgaggaggag
     1501 atcgagagcg tcgacagcgc cttcatgaag cgcggcctgc gcatggtagt agactcgacc
     1561 gatccggaag tgatcaagaa catcctggag acggagctgt cccagatgaa cgagcgccac
     1621 ggccgctggc tcaagatgat cgaccagtgg gccaagctgg ccccgggcat gggcatgctc
     1681 ggtacggtcc agggcctcat cgcgatgatg aagaacctcg aggacaagag ccgcatcggc
     1741 cccaacatgg ccgtggccct gatcacgacg tactacggcg ccatcatggc caacttcctg
     1801 tttaccccta tgatgggcaa gctcgccaat caggacgcgg ccgagacgaa ggtccgcgag
     1861 atggtcatag aaggcgtcct gtccatccag cagggcgata acccgcatat ccttcagatg
     1921 aagctctcct cctatctgtc tcccgagagc cagaagaagc tcgaagagct gcatccgcag
     1981 tcataaggcg gcgtaggcat ggctcgcaag aagaaagaga ccgccctcgg cggcactgga
     2041 gaatggatcg tcacctacag cgacatggtc acgctgctcc tgtgcttctt cgtggccctc
     2101 ttcgatacga ccgaggtgga cgtggtccag ctcaaccaga tgatatccag cctgaacaac
     2161 ataggcatgg gcgcgagcac cgggggcaat accctgtcgg tcgggcgcat ggccgagctc
     2221 ggcaactcga tcaacagcct gcc
//
LOCUS       DAOPHV010000464         2243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_68453_length_2243_cov_2.873400, whole genome shotgun sequence.
ACCESSION   DAOPHV010000464 DAOPHV010000000
VERSION     DAOPHV010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68453_length_2243_cov_2.873400"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="PLQ29_10890"
     CDS             <1..1415
                     /locus_tag="PLQ29_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87194.1"
                     /translation="STNNLNDTKLSYNKEALANRTETAYWNDDRLVRVWQSNTADFFA
                     GSENPVFPAMSMDAVGELYASFSNYSTAKVYYSGIGGTATEVFYSFDPSEETSITVSG
                     SGATRQINVLYSANYHGGNEEDWVADADQAGGLYLYDDSAAAYYVGRDSRRAYRFELF
                     YHDRMLQQFKNLRVARASANSGERIHVAYYDRITNAIKYSNINDGYVVPTTGAMTKYS
                     SHEISWVKIDGGSDAHDTAAYSEGSSIALGAARFETGLQVSSGTGEMSAIALTSTKLP
                     VVVYYDAANSVLKLARANSTNPKGNQARWTVQSVLPAGDANAGTVVDYIAAKIDSEDY
                     IHIAFQNSKGELVYLRSTNNPTDGTTRYEFSPSQVLAGSAMWTDLTLRGTTPYISYLS
                     RINSFDGVHITFYDASLDLDGDGTAEGGWETMTAAMNYKASNIRTCVAAHPTPATGGW
                     TAAVSFTPGNLYRVVRYIGF"
     gene            1505..>2243
                     /locus_tag="PLQ29_10895"
     CDS             1505..>2243
                     /locus_tag="PLQ29_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87195.1"
                     /translation="MGKRGFVLTAVVAACWLLAVGVMARAVSPEPPPPVEPGATVAGH
                     KPDDTRRQRRISVAAAALRPEALADVSAGESKARARALASRALAAGLEPAATVARALP
                     GSTWVQARLVMRRDEENLRLDAALDNAYGRSYAAIRAGLAETRATAEAVEAALGELWR
                     VFYDDARTDGSRGGPLVSPLARRDQWIPGTRALRASHQYALDIFFTSLSRDGAEEKGP
                     TVVSMSRGVVVSDAGDWVGGDRPSAYVS"
BASE COUNT          448 a          734 c          701 g          360 t
ORIGIN      
        1 gctccacgaa taacctgaac gataccaaac tctcctataa taaagaagcg cttgccaaca
       61 ggacggagac cgcctactgg aacgacgacc gtctcgtcag ggtatggcag tccaataccg
      121 cggatttctt cgcgggtagc gaaaaccctg ttttcccggc catgagcatg gacgccgtcg
      181 gagaactcta cgcgtcgttc agcaactatt cgacggccaa ggtctactac tccgggatag
      241 gcggcacggc gaccgaggtg ttctattcct tcgacccgtc ggaagaaacg tccattacgg
      301 tatcgggctc gggagctacc cgccagatca acgtactgta ttcggccaac taccatggcg
      361 gcaacgagga ggactgggta gccgacgcgg atcaggccgg agggctctac ctctacgacg
      421 atagcgctgc cgcatactat gtcgggcgag atagccgcag agcctatcgc ttcgagttat
      481 tctaccatga caggatgctg cagcagttca agaacctccg agtcgccagg gcgagcgcca
      541 attcgggaga acggatacac gtagcatact acgaccggat aaccaacgcg attaaatact
      601 caaacataaa tgacggttac gtggtaccta ccaccggagc gatgactaaa tacagtagcc
      661 acgagatcag ctgggtcaag atcgacggcg gatccgacgc ccatgacacg gccgcctata
      721 gcgaaggtag ctctatcgcc ctcggcgcgg ccaggttcga gaccgggcta caggtctcga
      781 gcggaacggg agagatgtcc gccattgcgc taacctcgac gaagctaccg gtcgtcgtgt
      841 actacgatgc cgccaacagc gttctcaagc tggcccgggc caacagtacc aaccccaagg
      901 gcaaccaggc tcgctggacc gttcagtcgg tgctccccgc gggggacgcc aacgctggta
      961 ccgtggtcga ctatatagcc gcgaagatcg atagcgagga ttatatacat atagccttcc
     1021 agaacagcaa gggagagctc gtctacctga ggtcgacaaa caacccgacg gacggaacta
     1081 cacgctacga atttagcccg agccaggtcc tcgccggatc tgcgatgtgg accgacctaa
     1141 ccctacgcgg cacaacgccc tacatatcgt acctatcgag gatcaactcg ttcgacggcg
     1201 tccacatcac cttctacgac gccagccttg atctggacgg tgacggaacc gcggagggcg
     1261 gatgggagac catgaccgcg gccatgaact ataaagccag caatatacgt acatgcgtgg
     1321 cggcccatcc gacgccggcg acgggaggct ggacagcggc cgttagcttt acaccaggca
     1381 acctataccg cgtcgtgcgc tatatcggat tctgagttcc tggctaatcc ccttcccttg
     1441 accccgctcc ctcccccggc tagactgact ggccggccgg aggggcggcg ggaaggcggg
     1501 cttcttgggc aagcgcgggt tcgtactaac ggccgtcgtg gcggcgtgct ggctgctcgc
     1561 cgtcggggtg atggcccgcg cggtgtcccc cgagcctccg cccccggtcg agcccggagc
     1621 gaccgtcgcc ggccataaac ccgacgacac gcggcggcag cggaggatct cggtagccgc
     1681 cgcggccctg cggccagagg cgctcgcgga cgtatcggcc ggcgagtcga aggcccgggc
     1741 gcgggccctc gcgagcaggg ccttggcggc cgggctcgag ccggccgcta cggtcgcccg
     1801 cgccctgccg ggctcgacct gggtccaggc ccgcctggtc atgcgccgcg acgaggagaa
     1861 ccttaggctg gacgcggccc tggataacgc ctacggcagg tcgtacgcgg ccatccgcgc
     1921 cggtctcgcg gagacccggg ccacggccga ggcggtcgag gccgccctag gcgagctatg
     1981 gcgcgttttt tacgacgacg ctcggaccga cgggagccgc ggcgggccgc tcgtctcgcc
     2041 gctggcccgc cgcgatcagt ggatacccgg aaccagagcc ctccgcgcgt cccaccagta
     2101 cgccctcgac atcttcttca cgagcctgtc gcgcgacggc gccgaggaga aaggcccgac
     2161 ggtcgtcagc atgagccgcg gcgtcgtcgt ctcggacgcg ggcgactggg tcggcggcga
     2221 ccgtccctcg gcgtacgtct cgg
//
LOCUS       DAOPHV010000465         2240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_68575_length_2240_cov_3.749657, whole genome shotgun sequence.
ACCESSION   DAOPHV010000465 DAOPHV010000000
VERSION     DAOPHV010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68575_length_2240_cov_3.749657"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /gene="eno"
                     /locus_tag="PLQ29_10900"
     CDS             complement(<1..442)
                     /gene="eno"
                     /locus_tag="PLQ29_10900"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739795.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPG87196.1"
                     /translation="MSIIEYVEAREILDSRGNPTIEVDVVLEDGTLGRAAVPSGASTG
                     EYEAVELRDGDKKRYLGKGVLKAVENVNNILAGELCGLDVTEQVDIDRTMIEVDGTPN
                     KGKLGANAILGASMACARAAAEYLDMPLYKYLGGAHTTLLPVPMA"
     gene            complement(577..1866)
                     /locus_tag="PLQ29_10905"
     CDS             complement(577..1866)
                     /locus_tag="PLQ29_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HPG87197.1"
                     /translation="MEHYDVAIIGTGPAGLGAAFSLLEARPGLRLALVDRNQYSSGGL
                     RNDCKMNFSWPVGFPEDDWDRAGAERYLERVEAFLSPEIMGRYDVDVYARRAERLGCS
                     LLEIRQAHLGTDGGLALIKRLVADLERRGAAPSLDETVLSVDAASRVIVTDRRELSFR
                     AVLVAPGRAGFAFLQDVMRANGVPFVDNVVDVGVRVELREDRYPIVKDYYDPKFLFPK
                     KVRTFCTNSRAAHVVQERYEDEAGAWYSVNGHAWSKDRPANGLANFAMLKTIALTEPL
                     ASGQAYARALGAQAMLLGGGRPIMQRVGDFRLGRRSTREAFTGDLYNFAPTLPSCTPG
                     DLGLAVPAKVMRALWNALKSLDTIVPGVLHPSTVLYYPEIKTYANKPVFQDPASFRAA
                     PGLYLAGDGAGTSRGITAAWASGMRVADGMLGELGER"
     gene            complement(1904..>2240)
                     /locus_tag="PLQ29_10910"
     CDS             complement(1904..>2240)
                     /locus_tag="PLQ29_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87198.1"
                     /translation="YYKAGFRGLGIEISYGSVFLTAVDRLDGGAVGIRSLMLDSLDDR
                     SFEHYTSTYLAPVVVDGRPMTFVRYFNRPGLRKPGLGLLQVLNLFVSPEAKAQLEALE
                     GETRRRLER"
BASE COUNT          354 a          721 c          791 g          374 t
ORIGIN      
        1 tggccatggg cacgggcagg agcgtggtgt gggctccgcc caggtacttg tacagcggca
       61 tgtccagata ctcggcggcc gcacgggcgc aggccatgga cgcgcccagg atggcgtttg
      121 cgccgagttt gcctttgttg ggggtgccgt ctacctctat catggtgcgg tctatatcca
      181 cctgctcggt gacatccagg ccgcatagct cgccggccag tatgttgttc acgttctcca
      241 cagccttaag cacacccttg cccaggtagc gcttcttgtc gccgtcgcgt agctccacgg
      301 cctcgtactc gccggtcgag gcgccggagg ggacagcggc gcggccgagg gtgccgtcct
      361 cgaggacgac gtccacctcg atggtgggat tgccgcggga atcgaggatc tcgcgggctt
      421 ctacgtattc gatgatgctc atgacgggct ccttaacgtt gaggatgacg acgagccggc
      481 gagccggccc cgatgcagta ctatcgtgta ttctcgaaca gtaacttttt taggtaccac
      541 agtcaaggat agtcgcgagc ttgcatgcct ttgagtctat cgctcgccga gctcgccgag
      601 catcccgtcg gcgacgcgca taccagaggc ccaggcggcg gttattccgc ggctcgtgcc
      661 ggcgccgtcg cccgccaggt agaggccggg cgccgctcgg aagctggccg ggtcctggaa
      721 gaccggcttg ttggcgtagg tcttgatctc cgggtagtac agcaccgtgc tcgggtgcag
      781 cacgcccggc actatcgtgt ccaggctctt gagggcgttc cagagcgcgc gcatcacctt
      841 ggccggcacc gccagaccga ggtcgccggg cgtgcaggac ggcagcgtcg gcgcgaagtt
      901 gtacaggtcg cccgtgaagg cctcgcgggt ggagcgccgg ccgaggcgga agtcgccgac
      961 gcgctgcatg atcgggcgcc cgccgcccag gagcatcgcc tgggcgccga gcgcgcgcgc
     1021 gtaggcctgg cccgaggcga gcggctcggt cagcgctatc gtcttgagca tggcgaagtt
     1081 cgcgaggcca ttggccggcc ggtccttgga ccaggcgtgg ccgttcaccg agtaccaggc
     1141 gcccgcctcg tcctcgtacc gctcctgcac gacatgggcc gcgcgggagt tggtacagaa
     1201 ggtacggacc ttcttgggga acaggaactt ggggtcgtag tagtccttga cgatgggata
     1261 gcggtcctcg cgcagctcga cgcgcacgcc gacgtcgacc acgttgtcga cgaagggcac
     1321 gccgttagcg cgcatcacgt cctgcaggaa ggcgaagccg gcgcggcccg gggcgacgag
     1381 gaccgccctg aacgacagct cccggcggtc ggtgacgatg acccgggaag ccgcgtccac
     1441 cgacaggacg gtctcgtcga gagacggggc ggcgccgcgc cgctcgaggt cggcgacgag
     1501 gcgcttgatc agcgccaggc cgccgtcggt gcccaggtgg gcctggcgta tctcgagcag
     1561 gctacagccg aggcgctcgg cgcggcgcgc gtacacgtcg acgtcgtagc ggcccatgat
     1621 ctctggcgac aggaaggcct ccacccgttc cagatagcgc tcggcgcccg cccggtccca
     1681 gtcgtcctcc gggaagccga caggccagct gaaattcatc ttgcagtcgt tgcggaggcc
     1741 gccggaggag tattggttgc ggtcgacgag ggctaggcga aggccgggtc gggcctcgag
     1801 gagggagaag gccgcgccga gtccggcggg tccggtgccg atgatggcta cgtcgtagtg
     1861 ttccataccg gtaccgcatg ctagcagatg cgccggcctt gtactagcgc tccagccgcc
     1921 tgcgggtctc gccctcgagc gcctcgagct gggctttggc ctccggcgag acgaacaagt
     1981 tgagcacctg gaggagccca agacccggct tgcgcaggcc gggccggttg aagtagcgaa
     2041 cgaaggtcat cggcctgccg tcgacgacaa ccggcgccag gtaggtggac gtatagtgct
     2101 cgaaggagcg gtcgtccagg ctgtcgagca tcaggctccg tatcccgacc gcccccccgt
     2161 ccagccgatc cacggccgtc aggaagaccg acccgtagct gatctcgatg ccgaggccgc
     2221 ggaagcccgc cttgtagtat
//
LOCUS       DAOPHV010000466         2239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_68615_length_2239_cov_4.151099, whole genome shotgun sequence.
ACCESSION   DAOPHV010000466 DAOPHV010000000
VERSION     DAOPHV010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68615_length_2239_cov_4.151099"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLQ29_10915"
     CDS             <1..594
                     /locus_tag="PLQ29_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87199.1"
                     /translation="HDTGYIRTVDEDGGTGARFTAVHVARSVAFAVDKAASFGLGPAS
                     AERVARLVYATGTKGEYGEQAWAGFDERQAGALIASADLVGQMSDRAYLEKLLFLYYE
                     FKEAGFPGYETEFDMLRKTMGFYDMTVALLDDKYGGVRSSARRHFAERWGIDRDLYAE
                     SMDRQMAYLQGILDDATTNFRKKLKRMDLELSQPRTA"
     gene            725..1534
                     /locus_tag="PLQ29_10920"
     CDS             725..1534
                     /locus_tag="PLQ29_10920"
                     /inference="COORDINATES: protein motif:HMM:NF027589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HPG87200.1"
                     /translation="MKALCVIADLVDSRGIADRGRFQRGLKASLEALSSRRGARLLSP
                     YTITLGDEFQALYRDSGTLLADFAWLAWAIFPQRARLSAAYDDISTDSNVREALGMDG
                     LAFHAARDALEGLKAEKRMILDVSWPGSPWVALVNASLRSFAHGMEGWNATAVCAFAG
                     LMNGLSLDEVAEEAGVTRRMVYKAMATRGLRDAAALFAAVAAGLGRLEKEPPGRVGPG
                     YRPGRYGAACSSPWAWSCAPSRRTTWSWRRSNASQPFPKTEPGTIGRAWAS"
     gene            1522..1671
                     /locus_tag="PLQ29_10925"
     CDS             1522..1671
                     /locus_tag="PLQ29_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87201.1"
                     /translation="MGQLIGALERTILYVLIVSGNFTEYFLVGTMASVCVTLVLGLLV
                     RLALA"
     gene            complement(1673..>2239)
                     /locus_tag="PLQ29_10930"
     CDS             complement(1673..>2239)
                     /locus_tag="PLQ29_10930"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HPG87202.1"
                     /translation="GVYLGDYPLITGNSPWKDRLGEQVVSPLVTIRSEPSGPRVEIGY
                     GYTGDGFRAENCPIIEGGRLANYTIGFYASNKTGKARCPSGGGAVVVEPGETALEDMI
                     RDVKRGILLARFSGGNPSDNGDFSGVAKNSYLIEDGRIVRPVGETMIAGNVAKLFESV
                     RAVSAETVDYGSGVLPWMLAGGVSVSGK"
BASE COUNT          357 a          721 c          763 g          398 t
ORIGIN      
        1 cacgataccg gctatatacg aacagtggat gaggatggcg gtaccggagc ccgcttcacc
       61 gccgtccacg tagcccggtc cgttgccttc gccgtcgaca aggcggcgag cttcggcctc
      121 ggccccgcta gcgccgagcg cgtcgcccgg ctcgtgtacg ccacgggcac caagggcgag
      181 tacggggagc aggcctgggc cggcttcgac gagaggcagg ccggcgcgtt gatcgcgagc
      241 gccgatctcg tcggccagat gtccgaccgc gcgtacctcg agaagctcct gttcctctac
      301 tacgagttca aggaggccgg gttccccggc tacgagaccg agttcgatat gctgcgcaag
      361 acgatgggct tctacgacat gaccgtcgcc ctgctcgacg acaagtacgg cggcgtccgc
      421 tcctcggcgc gccggcattt cgccgagcgt tgggggatag accgcgacct ctacgccgag
      481 tcgatggacc gccagatggc ctacctgcag ggcatcctcg acgacgccac gaccaacttc
      541 cgcaagaagc tcaagcgcat ggacctggaa ctgtcccagc cgaggacggc gtagggcggg
      601 cgacgaggcg tacgagcttc acctgcgagg agtgggtatc tctgtgcgta tttagttctc
      661 tctttaaaaa cgaactaaaa ttaacacatg acagctgatg aaccatagtg gtactcttga
      721 ttccgtgaag gctctctgtg tcatcgcgga tttagtcgac tcgaggggca tcgccgaccg
      781 cgggcgcttc caacgaggcc ttaaggcgag tctcgaggcc ctgagctcgc ggcggggagc
      841 caggcttctc tcgccatata cgatcacgct gggggacgag ttccaggctt tgtatcgcga
      901 ttccggcacc ctgctcgccg atttcgcctg gctcgcctgg gccatcttcc cgcaacgggc
      961 gcgcctatca gccgcctatg acgatataag caccgacagc aacgttcgtg aggccctcgg
     1021 gatggacggg ctggcgttcc acgcggcgcg cgacgcgctc gagggcctaa aggccgagaa
     1081 acgtatgatc ctggacgtga gctggccggg ctcgccgtgg gtagcgctcg tcaacgcgtc
     1141 gctccgttcc ttcgcccacg gtatggaggg ctggaacgct accgccgtct gcgcgttcgc
     1201 aggtctcatg aacggactgt ccctggatga ggtcgcggag gaggcgggcg tgacgcggcg
     1261 catggtctac aaggccatgg cgacgagggg gctccgcgac gcggccgcgc tcttcgcggc
     1321 ggttgcggcc ggcctcgggc gcctggagaa ggagccgcct ggccgggttg gccctggcta
     1381 tcggccgggg cgctacggcg cggcctgctc gtcgccgtgg gcctggagct gtgcgccgtc
     1441 gaggcgaact acctggtcgt ggcggcgctc aaacgcttct cagcctttcc caaaaacgga
     1501 gccagggacg ataggccggg catgggccag ctgatcggcg cgctcgagcg gaccatcctg
     1561 tacgtgctca tcgtctcggg caacttcacc gagtacttcc tggtaggtac catggcctcg
     1621 gtctgcgtca cgctggtgct gggcctgctc gtacggctgg ccctggcgta gcctacttcc
     1681 ccgacacgct gaccccgccg gctagcatcc agggcaggac gccggagccg tagtcgacgg
     1741 tctcggcgga cacggcgcgg accgactcga agagcttggc cacgttgccg gctatcatgg
     1801 tctcgccgac cgggcgtacg atacggccgt cctctatcag gtaggagttc ttggccacgc
     1861 cggagaagtc gccgttgtcc gacgggttgc cgcctgagaa gcgcgcgagc aggatgccgc
     1921 gcttgacgtc gcgtatcatg tcctcgaggg ctgtctcgcc cggctcgacg acgacggcgc
     1981 ctccgcccga ggggcagcgg gccttgccgg tcttgttcga cgcgtagaag cctatcgtgt
     2041 agttggccag gcgtccgccc tcgatgatgg ggcagttctc ggcgcggaag ccgtcgccgg
     2101 tgtagccgta gccgatctcg acgcgcgggc cgctcggctc ggagcgtatg gtcacgagcg
     2161 gcgagacgac ttgctcgccg agcctgtcct tccatggcga gttgccggtg atcagcgggt
     2221 agtcgccgag gtagacgcc
//
LOCUS       DAOPHV010000467         2239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_68619_length_2239_cov_3.625000, whole genome shotgun sequence.
ACCESSION   DAOPHV010000467 DAOPHV010000000
VERSION     DAOPHV010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68619_length_2239_cov_3.625000"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="PLQ29_10935"
     CDS             <1..1288
                     /locus_tag="PLQ29_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87203.1"
                     /translation="YYERGELAEAREVLEGSIALDRSDARAWFLLGLVEQAAGRRAEA
                     EYALSTLAALRPEDELARIALENLVIEGTGFEDPSRSALAAWRFSRAAEFERRLLYQK
                     AIAEYRRGLLVDPYANPGRRRYAELLRGSGLPSSYLAELRFLDDLGKSDRALSEAIEI
                     YDSLLAGSVGRDWSVGLGARTDPYRLVVFSVAPDASTYHAGGDLVVARYLRDSLAFEP
                     GLAPDRGVARVSGFADAFALARESGAEWFVLVRVAETERDILVSAELRAARTGALAAR
                     VDAPRSGNDRVALSTARVVQGIREALPTRGSLLERRGDLALVGLGRVDGVAVGDAFIL
                     VKRGAVAIEPDGGGLAWKDSDVVARVVASRVDDELFEGKLERVGFFDRVNPRDVALRE
                     PPKPKPEAAQDSGGEASAAKASYVWSALFERVRSLY"
     gene            1506..>2239
                     /locus_tag="PLQ29_10940"
     CDS             1506..>2239
                     /locus_tag="PLQ29_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HPG87204.1"
                     /translation="MNAYIREVLDGLKARAPWETEFLQAAEEVLESIEPLIEKNPKYK
                     AQRILERIVEPERTIMFRVPWMNDKGEYIVNRGYRVQFNSAIGPYKGGIRFHPKVTLS
                     TLKFLGFEQTFKNSLTSLPMGGGKGGADFDPNGKSDNEIMRFCQSFMTELQKYVGPDT
                     DVPAGDIGVGGREVGYMFGQYKRLSDEFTGVLTGKGLSFGGSLIRPEATGFGAVYFLN
                     EMLKAKGDTLKGKTVAIQKNGPHVDY"
BASE COUNT          356 a          764 c          744 g          375 t
ORIGIN      
        1 gtactacgaa cgcggcgagc tggccgaggc tcgcgaggtg ctcgagggtt ccatcgccct
       61 cgaccggagc gacgcccgag cctggttcct gctcggcctg gtcgagcagg cggccggaag
      121 gcgcgccgag gccgagtacg cgctgtcgac gctcgcggcc ctgcgtcccg aggacgagct
      181 ggccaggata gcgctcgaga acctggtcat cgagggtacc ggcttcgagg atccgtcgcg
      241 ctccgccctg gcggcctggc gcttctcccg ggcggccgaa ttcgagcggc ggctactcta
      301 ccagaaggcc atcgccgagt accggcgcgg cctcctcgtc gatccctacg ccaatcctgg
      361 ccgcaggcgc tacgccgagc tgctccgcgg ttccggcctg ccttcgtcct acctggccga
      421 gctgcgcttc ctcgacgacc tcggcaagtc cgaccgggcc ttgtccgagg cgatcgagat
      481 ctacgacagc ctgctcgccg ggtcggtcgg ccgggactgg agcgtcggcc tcggcgcgcg
      541 gaccgatccg taccgcctcg tcgtcttctc ggtcgccccc gacgcctcca cgtaccacgc
      601 gggcggcgac ctggtcgtgg cgcgctacct ccgcgactcg ctcgccttcg agccgggcct
      661 ggcgccggat cgcggcgtgg cgagggtctc cggcttcgcc gacgcgttcg ccctggccag
      721 ggagtcgggc gccgaatggt tcgtgctcgt gcgcgtcgcg gagacggagc gcgatatcct
      781 cgtgtcggcc gagctacggg cggcgaggac gggggccttg gccgcccgtg tcgacgcgcc
      841 tcgctccggt aacgaccgtg tggccctatc gaccgcccgg gtcgtccagg gcatacgcga
      901 ggccctgccg acgcgcggct ccctgctcga gcgtcgcggc gacctggccc tcgtcgggct
      961 gggccgggtc gacggcgtgg ccgtcggcga cgccttcatc ctcgtcaagc gcggagccgt
     1021 agccatcgag cccgacggcg gcggcttggc ctggaaggac tcggacgtcg tggcgcgcgt
     1081 ggtcgcctcg cgggtcgacg acgagctgtt cgagggtaag ctcgagcggg tggggttctt
     1141 cgaccgcgtc aacccgcgcg atgtggcgct gcgagagccg ccaaagccga agccggaggc
     1201 cgctcaggac tcaggcggag aggcctcggc tgccaaggcg tcgtacgtct ggagcgccct
     1261 gttcgagcgg gtgcgtagcc tctactaacg cccgccctcc tccgtagcgt tgtacccatc
     1321 tgccacgact cgcggcgctt gcccggtcgt ggtgtaccgt cgatagtgat tttgatatat
     1381 gaaataccgc tatcaataat cgaaaaacgt cttgcataga ctccgccgaa tgtgtacaat
     1441 gttccatgga cggaccagtg tccgtaccga gcatcgtgtc tgtcagctct attaaaggag
     1501 ggcgaatgaa cgcttatatc cgcgaggtcc tcgatggcct caaagcccgc gcgccgtggg
     1561 agaccgagtt ccttcaggcc gccgaggaag tcctcgagtc gatcgagccg cttatcgaga
     1621 agaaccccaa gtacaaggcc cagcgcatcc tcgagcgcat cgtcgagccc gagcgcacca
     1681 tcatgttcag ggtgccctgg atgaacgaca agggcgagta catcgtcaat cgcggctatc
     1741 gcgttcagtt caacagcgcc atcggcccgt acaagggcgg tatccgcttc cacccgaagg
     1801 tgacgctctc caccctcaag ttcctcggct tcgagcagac cttcaagaac agcctcacga
     1861 gcctcccgat gggcggcggc aagggcggcg ccgacttcga cccgaacggc aagagcgaca
     1921 acgagatcat gcgcttctgc cagagcttca tgaccgagct tcagaagtat gtcggccccg
     1981 ataccgacgt gcccgctggc gacatcggcg tgggcggccg cgaggtcggc tacatgttcg
     2041 gccagtacaa gcgcctgagc gacgagttca ccggcgtgct gaccggcaag ggcctgtctt
     2101 tcggcggctc gctgatccgc cccgaggcta ccggcttcgg cgccgtgtac ttcctgaacg
     2161 agatgctcaa ggccaagggc gacacgctca agggcaagac cgtcgcgatc cagaagaacg
     2221 gcccgcacgt cgattatta
//
LOCUS       DAOPHV010000468         2236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_68770_length_2236_cov_3.186153, whole genome shotgun sequence.
ACCESSION   DAOPHV010000468 DAOPHV010000000
VERSION     DAOPHV010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68770_length_2236_cov_3.186153"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /locus_tag="PLQ29_10945"
     CDS             <1..1749
                     /locus_tag="PLQ29_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87205.1"
                     /translation="AREGRLSLQAVQNRVDLIYLPEAFTETLLAEYRRIEENPDIPFP
                     DEDSMRLSVPPEWIQAVSVEADLPSLVAFEGDRPVPIYRLVFPEGLKPVVILSVLVGD
                     KLLEYAILKLRNYLRKGSNRDFIQQRLAGAFSGKDRMLKDALTSILIKPFDSLQAMKQ
                     GAGDFSYSFWAYLTTAVRKDLSGKSELMPDDIAAYQASYVVDVFNNHYKNLAQRGQER
                     ESAFKSLSVMLRKPPYLYSIDQIVDFRDAQGRPLLGKYSREELEAWIQERTTKAAEGA
                     LPELLILGSTGGSSGQVIAKETLIPYLVKALREARASIKTTMTRDWRDVLADFGSTIS
                     MDDDAAFRTELEKRVEAHSPTLSSILITALPMLVYQESRNGREPSAELDRCFGGSRMA
                     GIDVLLDLDRKRLLADVKMMLPIWYSIPIVSWLLKLFLKGAKRRAGKRAVEAAAQSAP
                     GQERAGQAGQAGQAGQAPANSRAMEFSQMARAAEKAMVPAGRTLDEQLRVLAARWNTL
                     LDPAAKANLTEDINSLVRDYLRTTLRSMRPSAFTPDRVEMMAANLADRPNLLKIRNHQ
                     ALEEYIRLYMVKMLKR"
     gene            1862..>2236
                     /locus_tag="PLQ29_10950"
     CDS             1862..>2236
                     /locus_tag="PLQ29_10950"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HPG87206.1"
                     /translation="MNASLLGVAFCLVFSALFSSVETAFTSLSLFQIEALKKRGRGGG
                     IVERLARKPDELISTILIGNNVVNITASALATSWALGLWGDWVLGLVTGVMTLIVLIF
                     GEVTPKRIALAHNEGVALALAPF"
BASE COUNT          385 a          762 c          729 g          360 t
ORIGIN      
        1 gccagggagg ggcggctgtc cctgcaggcc gtccagaacc gcgtcgacct gatctacctc
       61 cccgaggcct ttaccgagac gctattagcc gagtaccgcc gcatcgagga gaacccggac
      121 atacccttcc ccgacgagga ctcgatgcgc ctgtcggtgc ctcccgaatg gatacaggcg
      181 gtatcggtcg aggccgacct gccgtccctg gtcgccttcg agggcgaccg gcccgtgccg
      241 atctaccgat tggtgtttcc cgaggggctc aagcccgtcg tgatcctatc ggtactggtc
      301 ggcgataagc tgctcgagta cgcgatcctg aagctacgca actacctccg caagggctcg
      361 aaccgcgact tcatccagca gcggctggcc ggggccttct ccggcaagga tcgcatgctc
      421 aaggacgcgc tgacgagcat cctgatcaag cccttcgact cgctgcaggc gatgaagcag
      481 ggcgccgggg acttctcgta ctcgttctgg gcctacctga ccacggccgt acgcaaggac
      541 ctgtcgggca agagcgagct catgcccgac gacatcgccg cctaccaggc gtcctacgtc
      601 gtcgacgtgt tcaataacca ttacaagaac ctggcccagc gcggccagga acgcgagtcg
      661 gccttcaagt cgctgtcggt catgctccga aagccgccct acctgtactc gatcgaccag
      721 atcgtcgact tcagggacgc gcagggccgg cccctgctcg gcaagtacag ccgggaggag
      781 ctcgaggcct ggatccagga gcggacgacc aaggcggccg agggcgcgct gccggagctg
      841 ttgatcctcg gctcgacggg cggctcgagc ggccaggtca tagccaagga aaccctgatc
      901 ccctacctgg tcaaggcgtt gcgggaagcg cgcgcctcga tcaagacgac catgaccagg
      961 gactggcggg acgttctcgc cgacttcggc tcgaccatct cgatggacga cgacgcggcg
     1021 ttcaggaccg agctcgagaa gcgcgtcgag gcccattcgc cgacgctctc gtcgatactg
     1081 atcaccgcgc tgccgatgct cgtgtaccag gagagccgca acggccgcga gccgtcggcg
     1141 gaactggacc gctgcttcgg cggttccagg atggccggga tcgacgtact gctcgacctg
     1201 gaccgcaagc gcctcctggc ggacgtcaag atgatgctgc cgatctggta ctcgataccg
     1261 atcgtatcgt ggctactcaa gctgttcctc aagggcgcca agcgccgggc cggcaagcgg
     1321 gccgtcgagg cggccgccca gtcggcgccg gggcaggagc gggcgggcca ggcgggccag
     1381 gcgggccagg cgggccaggc gccggccaac tcccgggcca tggaatttag ccagatggcg
     1441 agggctgccg agaaggccat ggtgccggcc ggccgaacgc tcgacgagca gctgcgcgtc
     1501 ctggcggccc gctggaacac cctgctcgac cccgcggcca aggccaacct gaccgaggat
     1561 atcaactccc tcgtgcgcga ctacctgcgt accacgctca ggtcgatgcg gccgtccgcc
     1621 ttcacgcccg accgcgtcga gatgatggcc gccaacctgg ccgaccggcc gaacctgctc
     1681 aagatacgga accatcaggc gctcgaggag tatatccggc tctacatggt caagatgctc
     1741 aagcgatgag ggccggccca gcgtcggctc aggcgcgcgc tcggcttgcg ctaggcgcgg
     1801 ctatcgtata ggatcggctg ataatggacg atccataccc atctagcggc ggaggccgcc
     1861 cgtgaacgcg tcactgctgg gcgtagcctt ctgccttgtc ttctccgcgc tcttctcctc
     1921 ggtggagacg gcgttcacct ccctgtcgct gttccagatc gaggcgctca agaagcgcgg
     1981 ccgcgggggc ggcatcgtcg agcgtctggc ccgcaagccc gacgagctga tctcgaccat
     2041 cctgatcggc aacaacgtgg tcaacatcac cgcctcggcc ctggctacca gctgggccct
     2101 ggggctctgg ggcgactggg tcctgggcct ggtcaccggc gtgatgaccc tgatcgtgct
     2161 gatcttcggc gaggtgacgc ccaagcgcat cgccctggcg cacaacgagg gcgtcgcgct
     2221 ggccctggcg cctttc
//
LOCUS       DAOPHV010000469         2232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_68925_length_2232_cov_5.092788, whole genome shotgun sequence.
ACCESSION   DAOPHV010000469 DAOPHV010000000
VERSION     DAOPHV010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68925_length_2232_cov_5.092788"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PLQ29_10955"
     CDS             complement(<1..706)
                     /locus_tag="PLQ29_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPG87207.1"
                     /translation="MERRFVYMDYNATTPLRPEVKAEMIEDLELYANASSLHEAGRRA
                     RSRIERSRSAVAELIGAKDPERVIFTSGGSESNNTVFATMFHLGRTGGRKGIVTTKIE
                     HPCVLNAAKWLAEEGFPVTFLDVDEYGVVDMDQLKQAVGPETLLVSVMAANNEIGTVQ
                     DIKAIAAVAKAAGAYMHSDATQSVGKIPVSVDDWGVDYMTMSCHKLYGPKGIGGLYVR
                     EGCPLEPLIRGGHQEHG"
     gene            complement(712..1113)
                     /locus_tag="PLQ29_10960"
     CDS             complement(712..1113)
                     /locus_tag="PLQ29_10960"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPG87208.1"
                     /translation="MFSVSARTQYAIRAMTCLATRRERAATAAEIAQAENIPAKYLEG
                     ILSRLKSFGLIDSERGKNGGYRMAKDPSDITMLEIVEAIDGRIKPVTCVDAIGACSQG
                     AACHPRKFWIGLKSTIDAYLAERTLKDVVQG"
     gene            complement(1206..2054)
                     /locus_tag="PLQ29_10965"
     CDS             complement(1206..2054)
                     /locus_tag="PLQ29_10965"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87209.1"
                     /translation="MSALVSLLAGSLAFMAPLAPAAVGGLVTEYVGALNIALEGFILI
                     GAFSYAAVGAVAGPVVGFGAALAITAVAAAGADLFSRKAKADPFVVGLGVNMLAPAAA
                     SIVSQSLFATKGVAAVAGLQSTLVLPGLRISDIAAFAVAAAIILAIAATPFGTRSRAA
                     GMSPDSLAMAGLDYGKARGAAHLVSGLACGATGAALAASVGAWVPNMSAGRGWIALVA
                     VYLGGKRLGGTVAAVALFSVMLSAAASAQAVVELPAELLLSIPYFIAALVVIVGARRT
                     ARRRGT"
     gene            complement(2051..>2232)
                     /locus_tag="PLQ29_10970"
     CDS             complement(2051..>2232)
                     /locus_tag="PLQ29_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87210.1"
                     /translation="GSRPQALPFAALLFAWLDSGSRQAAILSDLPPDAGQAIKAIVIL
                     AVSARPAIAALRRRR"
BASE COUNT          389 a          741 c          737 g          365 t
ORIGIN      
        1 agccatgctc ctggtgcccg cccctaatga ggggctcgag cgggcagcct tcacgaacgt
       61 agaggccgcc tatgcccttg gggccgtaca gcttgtggca cgacatcgtc atatagtcga
      121 cgccccagtc gtcgaccgat acggggatct tgccaaccga ctgggtggcg tcggagtgca
      181 tataggcgcc ggcggccttg gccaccgcgg ctatcgcctt gatgtcctgg acggtaccga
      241 tttcgttatt agcggccatc actgacacga gcagggtttc agggccaacc gcttgcttga
      301 gctggtccat gtcgaccacg ccgtactcgt cgacgtcgag gaaggtaacc ggaaagccct
      361 cctcggccag ccatttggcc gcgttgagca cgcaagggtg ttctatcttg gtggtgacga
      421 tacccttgcg gccgcccgta cgcccgaggt ggaacatcgt ggcgaagacc gtattgttcg
      481 actccgagcc gccgctcgtg aagatgaccc gctcggggtc cttcgctcct atcagctcgg
      541 ccacggccga ccgcgagcgc tctatgcgcg agcgagcccg cctgccggct tcatggagac
      601 tcgaggcgtt ggcgtatagc tccaggtcct cgatcatctc cgccttgacc tcaggcctga
      661 gcggtgtggt cgcgttgtaa tccatgtaca cgaatcttcg ttccatcgtc gtcatccttg
      721 tactacgtcc ttgagcgtac gctcggcgag gtacgcgtct atggtcgact tgagcccgat
      781 ccagaatttc ctggggtggc aggcggcgcc ctgcgagcac gcgccaatcg cgtcgacgca
      841 ggtgaccggc ttgatgcggc cgtcgatagc ctcgacgatc tcgagcatgg tgatatccga
      901 cggatccttg gccatcctat agccgccatt cttgccgcgc tcgctatcga tgaggccgaa
      961 ggacttaagc ctcgaaagaa tgccctcgag gtacttcgcc ggtatattct ccgcctgggc
     1021 tatctcagcg gccgtcgccg ccctctctcg cctggtggcg aggcaggtca tggcgcgtat
     1081 ggcgtactga gtcctggccg atacggagaa catgcagata ccccttaatt cctatcagga
     1141 tactaggaat atatccccac tcgccatccc gagtcaagcc gagaggctga gtattcgtct
     1201 ggcggtcagg tgccgcggcg tcgggccgtg cgtcgggcac ctacgatgac gaccagggcc
     1261 gcgatgaagt atgggatgga caggagcagc tccgccggta gctcgacgac ggcttgggcc
     1321 gaggctgccg cagacagcat gaccgagaac agggcgacgg ccgcgacggt gccgccgagc
     1381 cgcttgccgc cgaggtatac cgcgacgagg gctatccagc cgcgcccggc gctcatattg
     1441 ggcacccagg caccgaccga cgcggccagg gccgcaccgg tcgcgccgca ggccaggccc
     1501 gatacaaggt gggccgcgcc tcgagccttg ccataatcga gcccggccat cgccagggaa
     1561 tcggggctca tgccggccgc ccgcgaccgc gtgccgaacg gggtcgcggc gatggccagg
     1621 atgatcgcgg ccgccacggc gaaggcggca atatcactga tcctcaggcc gggtaggacc
     1681 agggtcgatt gcagcccggc cactgccgcc acgcccttgg tcgcgaagag cgactgggat
     1741 acaatcgacg ccgcggccgg cgccagcata ttgacgccga gcccgacgac gaagggatcg
     1801 gccttggcct tgcgcgagaa caggtcggct ccggccgccg cgaccgccgt gatcgccaac
     1861 gcggccccga agcccacgac cgggccggcg acggccccga cggcggcgta cgagaacgcg
     1921 ccgatcagga tgaagccctc gagagcgatg ttcagcgcgc cgacgtactc cgtgacgagg
     1981 cctccgacgg ccgcaggcgc tagcggcgcc atgaaggcca gcgagccggc gagcaaggat
     2041 acgagcgcgc tcatcgccgc cgcctcagcg ccgcgatcgc tggcctggcg ctgacggcca
     2101 ggatgacgat cgccttgatg gcctgcccgg cgtcgggcgg caagtcggac aggatagccg
     2161 cctgcctcga gccggaatcg agccaggcga acaggagcgc cgcgaacggc aaggcctggg
     2221 gcctcgagcc gg
//
LOCUS       DAOPHV010000470         2231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_68960_length_2231_cov_4.753676, whole genome shotgun sequence.
ACCESSION   DAOPHV010000470 DAOPHV010000000
VERSION     DAOPHV010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68960_length_2231_cov_4.753676"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1941)
                     /locus_tag="PLQ29_10975"
     CDS             complement(<1..1941)
                     /locus_tag="PLQ29_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPG87211.1"
                     /translation="MFKGLTKRAQRVLTVVAQEEARRFHAGELAPEHVLLALLTEGEG
                     TGYRLLSSLKLDMRELALELERAASGRKGGFLLGDAPLSRRLKSMLETAAEEARTMDG
                     EYIGTEHLVIAAARENGSPFDQFLSRYGIFYEQLRAALKLLASPGPDEPRDRSDDAAR
                     QQARPVIQGTLGSAPRRSGGATPLLDEHSRDLTAWARDDRIDPVIGRETETRRVIRIL
                     SRRSKNNPVLIGDPGVGKTAIVEGLALRMAKGDVPDGLMGKRLLVLDMASIVAGTKYR
                     GEFEERLKRIMKEIAASGNVILFIDELHTIIGAGSAEGTIDASNMLKPALSRGELQCI
                     GATTLDEYRKYFEKDAALERRFQTVLVEEPTIEQTQDILSGIKERYERYHGVTYAADA
                     IEAAVRLAGRYVSDRFLPDKAIDVLDEAGARKKVEYPAKGPELSSIEEEIRRLTEEKL
                     ALVSTQNYERAAEVRDRVRELKASLESLRAKTKAASGDGYAVGVDDVRAVVSEMTGIP
                     LERIAEGESERLTSLEEELHRRVVGQDYAVKALAAAVRRSRAGISDPRRPLGSFIFLG
                     PTGVGKTLLAKTLAESLFGSEDALIRIDMSDFMEKHNAARLVGAPPGYVGYDEGGMLT
                     ERVRRKPYSVVLFDEIEKAHPDV"
     gene            complement(1934..>2231)
                     /locus_tag="PLQ29_10980"
     CDS             complement(1934..>2231)
                     /locus_tag="PLQ29_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87212.1"
                     /translation="TIGRAAGKAGGARLLSRDEATDICSGLRLGLACGVLEGLSLEDA
                     TDLWPGLRVKPSPSDASIGPSSQAGSEEPEAARRATSLRLAVAGLRLSQGYIDV"
BASE COUNT          332 a          774 c          716 g          409 t
ORIGIN      
        1 cacgtccgga tgggccttct cgatctcgtc gaacagcacc acgctatacg gcttgcgtct
       61 gacccgttcg gtcaacatgc cgccctcgtc gtaaccgaca tagcccggag gcgcgccgac
      121 gagcctggca gcgttatgct tttccatgaa gtccgacatg tcgatgcgga tcagcgcgtc
      181 ctcactgccg aacaggctct cggcgagggt cttggccagt agggtcttgc cgacgccggt
      241 cggtccgagg aagatgaagg agccgagggg acgcctcggg tcggagatgc cggctctgga
      301 tcgcctgacg gcggcggcga gcgccttgac cgcgtagtcc tggccgacca cccggcggtg
      361 cagctcctcc tcgaggctcg tcaggcgctc cgactcgccc tcggcgatgc gctcgagggg
      421 gattccagtc atctccgaga ccacggccct cacgtcgtcc accccgacgg cgtagccgtc
      481 gcccgaggcc gccttggtct tggcccgtag gctctcgagg ctggccttga gctcgcgcac
      541 cctgtcgcgc acctcggcgg cccgctcata attctgggtg gataccaggg caagcttttc
      601 ctcggtaagg cgcctgatct cctcctcgat ggagctcagc tccggcccct tcgccggata
      661 ctcgaccttc ttacgggcgc cggcctcgtc gagtacatcg atggccttgt ccggcaggaa
      721 acggtcgctg acgtatcggc cggcgagcct gacggccgcc tcgatcgcgt cggcggcgta
      781 cgtgaccccg tggtagcgct cgtagcgctc cttgatgccg gacaggatgt cctgggtctg
      841 ctctatcgtc ggttcctcga cgaggacggt ctggaagcgg cgctcgagcg cggcgtcctt
      901 ctcgaagtac ttgcggtact cgtcgagcgt cgtcgcgccg atgcactgta gctcgcccct
      961 cgacagggcg ggcttgagca tgttcgaagc gtcgatcgtg ccctcggcgc tacccgcccc
     1021 gatgatggta tgcagctcgt cgatgaagag gatcacgttg ccggaggcgg caatctcctt
     1081 catgatgcgc ttgaggcgct cctcgaactc gccgcggtac ttcgttccgg cgacgatgga
     1141 ggccatgtcg aggacgagca ggcgtttgcc cataagcccg tccggcacgt cgcccttggc
     1201 catcctgagg gcgaggccct cgacgatagc cgtcttgccg acgccggggt cgccgatcag
     1261 tacgggatta ttcttgctac ggcgcgacag gatgcgtatc acccgcctgg tctcggtctc
     1321 ccggcctatc accgggtcga tacggtcgtc gcgagcccag gccgttagat cgcgcgagtg
     1381 ctcgtcgagc agcggggtag cgccgcccga gcgcctcggg gccgagccga gcgttccctg
     1441 gatgaccggc cgggcttgtt gccgggcggc gtcatcggat ctgtcacgcg gctcgtccgg
     1501 cccgggcgaa gccaatagct tgagcgcggc acgaagttgc tcgtagaaga tgccgtaccg
     1561 cgataggaac tggtcgaacg gcgagccgtt ctcgcgcgcg gccgcgatga cgaggtgttc
     1621 cgttcctatg tactccccgt ccatcgtcct ggcttcctcc gcggcggtct cgagcatcga
     1681 cttgagccgc ctggacaagg gggcgtcgcc gagcagaaaa cctcccttgc ggccggaagc
     1741 ggcccgctcg agctcgagcg ccaactcgcg catgtccagc ttcagggacg ataggagtcg
     1801 atacccggtt ccttcgcctt cggttaacag ggccagtagc acgtgctccg gcgccagctc
     1861 gccggcatgg aaacgcctgg cctcttcctg agccacgacc gtgagcacgc gctgcgcccg
     1921 cttagtcaat cccttaaaca tcgatgtacc cctgcgataa acgtaggccc gcgaccgcga
     1981 gcctgagact cgtcgccctg cgcgccgcct cgggctcttc cgagcccgcc tggctcgaag
     2041 gcccaatact cgcgtccgac ggcgatggct tgacccgtag ccccggccac aggtcggtcg
     2101 cgtcctcgag gctcagcccc tcgaggacgc cgcaggccag cccgagccta agcccggagc
     2161 atatatccgt agcctcgtcg cgcgacagta gcctggctcc gcccgccttg ccagcggccc
     2221 gccctatggt a
//
LOCUS       DAOPHV010000471         2226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_69197_length_2226_cov_3.632888, whole genome shotgun sequence.
ACCESSION   DAOPHV010000471 DAOPHV010000000
VERSION     DAOPHV010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69197_length_2226_cov_3.632888"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..394
                     /locus_tag="PLQ29_10985"
     CDS             113..394
                     /locus_tag="PLQ29_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87213.1"
                     /translation="METRNGLINFTLFIFIFVFSFVFSLDALAAPSTFYGVLALLGFV
                     VCLAGSLFNGLLSKRDGEALAIWYFSYAVVVGIVTVWYMTRCGTAFGWW"
     gene            complement(503..>2226)
                     /locus_tag="PLQ29_10990"
     CDS             complement(503..>2226)
                     /locus_tag="PLQ29_10990"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPG87214.1"
                     /translation="SGYGVGLDDIRSFRQIGSKCAGHPEYGMAPGIETTTGPLGQGLA
                     TAVGMAMAETMLAARFNVAGRSIVDHYTWVLAGDGCMQEGVSAEAASLAGHLGLGKLI
                     VFYDDNAITIDGGTSLSFDEDVGARFKAYGWQVLKGDMYDAAAVRRMVAEAKAERGKP
                     SLIILKSVIGKGAPTMQGSHKVHGAPLGADEIAKAKAAMGLPADAEFWVSPEAKAFFA
                     ARRGELSAGRTAWQAEFTAWKTENPAKAAELEAMLAGKPLTAPAWPEAKVGDSVATRN
                     ASGAALKAAFSAWPGLVGGSADLTGPNVTALPCAPYSKADRSGRMIHYGIREHAMAAI
                     SNGLALHGGFKPFCATFLVFADYLRPSLRLAALMGLPVVYVMTHDSVFVGEDGPTHQP
                     IEHLASLRAIPNVLVLRPADAEETAEAWAWAMARTTGPTVIALTRQNLPVFEKADPMW
                     RDTFRAGAYVARTTRGEPDVVVLASGSEVELALKAAELASSKSVRVVSVPSLETFLAQ
                     PDALRDTIAPPEARIVACEAGRAMPWNGVADGFLGIERFGESGPGAKVAAHLGLTLEA
                     LAALINA"
BASE COUNT          333 a          751 c          752 g          390 t
ORIGIN      
        1 ttcatgctct cctcctcggc gaggtccggg cctcgatcaa gatggacaag tgctactcgt
       61 cggagtatac tacgatccga catctccaga tacgtaaaag gagtcgattg ccatggaaac
      121 caggaacggc ttgatcaatt tcacactctt catctttatc tttgtcttct cgttcgtatt
      181 ctcgctcgac gccctcgccg cgcctagtac attctacggc gtactcgccc tgctaggctt
      241 cgtcgtctgc ctagccggct ccctgttcaa tggcctgctc tccaagcgcg acggcgaggc
      301 cctggcgatc tggtatttct cctacgcggt cgtcgtcggc atcgtcaccg tctggtacat
      361 gacccgctgc ggcaccgcct tcggctggtg gtagtcggcc aacgatcggg tttagccaga
      421 aacggagggc cgccggcgat aatcgccggc ggccctcctc gtatatgcgg tagccccggg
      481 cgtaaccggg gctgccgctt cgttatgcgt taatcagcgc agccaaggcc tcgagcgtca
      541 ggccgaggtg ggcagcgacc ttggcgcccg gacccgactc gccgaagcgc tcgatgccga
      601 ggaagccgtc ggccacgccg ttccagggca tggcccgtcc ggcctcgcag gcgacgatgc
      661 gggcctcggg cggcgcgatc gtgtcgcgca gggcgtcggg ctgggccagg aaggtctcga
      721 gcgacggtac cgacacgacg cgcaccgact tggacgaggc gagctccgcc gccttgagcg
      781 ccagctcgac ctcgctgccg gaggcgagca cgacgacgtc cggctcgccc ctcgtggtgc
      841 gcgcgacgta ggcgcctgcc ctaaaggtgt cgcgccacat cgggtcggcc ttctcgaaga
      901 ccggtaggtt ctgcctggtc aacgctatga cggtcgggcc ggtcgtcctg gccatcgccc
      961 aggcccaggc ctcggcggtc tcctccgcgt cggccggcct gagcaccaac acgttcggga
     1021 tggcgcgcag cgaggccagg tgctcgatcg gctggtgcgt cgggccgtcc tcgccgacga
     1081 agaccgagtc gtgggtcatc acatagacga ccggcaggcc catcagggcc gccagcctga
     1141 ggcttggccg caggtagtcg gcgaatacga ggaaggtggc gcagaacggc ttgaagccgc
     1201 cgtgcagggc caggccgttt gaaatagccg ccatcgcgtg ctcgcggatg ccatagtgga
     1261 tcatcctgcc tgagcggtcc gccttcgagt acggcgcgca gggcagggcg gtgacgttcg
     1321 ggccggtcag gtcggctgag ccgccgacga ggcccggcca cgcggagaag gcagccttga
     1381 gagcggcgcc agaggcgttt cgcgtggcaa ccgagtcgcc gacctttgcc tcgggccatg
     1441 ccggcgcggt gagcggcttg cccgcgagca tcgcctcgag ctcggccgcc ttggccgggt
     1501 tctctgtctt ccacgccgta aattcggcct gccatgcggt gcgcccggcc gagagctcac
     1561 cgcgcctggc cgcgaagaag gccttggcct cgggtgacac ccagaattcg gcgtcggccg
     1621 gcaggcccat cgcggccttg gccttggcta tctcgtcggc gccgagaggg gctccatgca
     1681 ccttgtgcga gccctgcatg gtcggcgcgc ccttgccgat cacgctcttc aggatgatca
     1741 ggctcggctt gcccctctcg gccttggctt cggctaccat gcggcgcacg gccgcggcgt
     1801 cgtacatgtc gcccttcagc acctgccagc cataggcctt gaagcgcgcg cccacgtcct
     1861 catcgaagga tagcgaggtg ccgccgtcta tggttatggc gttgtcgtcg tagaagacga
     1921 tcagcttgcc gaggccaagg tggccggcga gtgacgccgc ctccgcggag acgccctcct
     1981 gcatgcagcc gtcaccggcg agtacccagg tgtaatggtc gacgatggag cggccggcga
     2041 cgttgaagcg ggcggcgagc atggtctccg ccatggccat accgacggcg gtcgccagac
     2101 cctggccgag cgggccggtc gtcgtctcga tgccgggggc catgccgtac tcggggtggc
     2161 cggcgcactt ggacccgatc tgcctgaagg accggatgtc gtcgagcccg accccgtagc
     2221 cggaga
//
LOCUS       DAOPHV010000472         2226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_69198_length_2226_cov_3.631506, whole genome shotgun sequence.
ACCESSION   DAOPHV010000472 DAOPHV010000000
VERSION     DAOPHV010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69198_length_2226_cov_3.631506"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1448)
                     /locus_tag="PLQ29_10995"
     CDS             complement(165..1448)
                     /locus_tag="PLQ29_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPG87215.1"
                     /translation="MTTHTKTLQERLAARTIRRPPAIVYLALANIWKALFIKKLGVRF
                     EYRIDLRDYRDGPYIVVSNHASRLDYIYAGIAFLPHRLNWVAGYNEFFRSHLAFVFRL
                     LQVIPKRNFTPDVYAIRQFSRIIRGGGKVIIFPEGMSSIGGSNQPCALGSGNLLKHFG
                     VPVLMTKIKGGYLTNTKYCLDERPGRVDVVVDLLFSPEDLERMSADEVQLALDRAIRQ
                     DDYAWNKVERVRFGGKGRMAHNLHHLLYWCPRCGAEFTMRGEGDELRCSACGNGARLN
                     EYYDLEPLDPSCVIPETPRVWFDLERARAREQVAADGFSLTERVRLGVLPRYEYLRDQ
                     KTSEIVGEGELRLDRGGLTYGGTKDGEPFGFHLNPDQVPTYGMCTDVTFFYTFHRNDY
                     YEFRPETESTAKWLLCTEEIHRLTGGAWKDFPEGR"
BASE COUNT          375 a          677 c          707 g          467 t
ORIGIN      
        1 cgcagcgagt caggttaaac gacggctagc gatcgccgcg ccagtcggcg atcgctaggt
       61 ttaagggtcg gattgccttt cggtcgtagc agttacagat accgatagac gcgttttatc
      121 ttgtcgatcc tgtcgccact tcttgattcc cgccttcgcg tcgtttatcg cccctcgggg
      181 aaatccttcc acgcgccgcc ggtcagccgg tggatctcct cggtgcagag gagccacttg
      241 gcggtcgact cggtctcggg cctgaactcg tagtagtcgt tcctatggaa tgtatagaag
      301 aaggtaacgt cggtgcacat gccgtaggtc ggaacctggt cggggttcag gtggaacccg
      361 aacggctcgc cgtccttggt gccgccgtag gtcaggccgc cccggtcgag gcgtagctcg
      421 ccctcgccga ctatctcgga ggtcttctgg tccctgaggt attcgtagcg gggcagaacg
      481 ccgagcctta cgcgctcggt cagggagaag ccgtcggcgg ccacctgctc gcgggccctg
      541 gctcgctcca ggtcgaacca gacgcgcggc gtctcgggta tgacgcacga cgggtcgagc
      601 ggctcgaggt cgtagtactc gttcagcctc gcgccgttgc cgcaggcgga gcatcgcagc
      661 tcgtcgccct cgcccctcat cgtgaactcg gcgccgcagc gcgggcacca gtacaggagg
      721 tggtgcaggt tgtgcgccat gcgtcccttg ccgccgaagc ggacgcgctc taccttgttc
      781 cacgcgtagt cgtcctgcct gatagcgcgg tccagcgcga gctgcacctc gtcggcgctc
      841 atgcgctcga ggtcctcggg gctgaacagc aggtcgacga cgacgtcgac gcgacccggc
      901 cgctcgtcca ggcagtactt ggtgttggtc aggtagccgc ccttgatctt tgtcatcagc
      961 accggaaccc cgaagtgctt gagcaggttt cccgagccga gggcgcaggg ctggttcgac
     1021 ccgccgatcg agctcatgcc ctcggggaag atgatcacct tgcctccgcc gcgtattatg
     1081 cgcgagaact gcctgatcgc gtagacgtcc ggcgtgaagt tgcgcttggg gatcacctgg
     1141 agcagcctga agacgaaggc caggtgcgag cggaagaatt cgttgtagcc tgcgacccag
     1201 ttcaggcggt gcggcaggaa cgcgatgccc gcgtagatat agtcgagcct ggaggcgtgg
     1261 ttcgacacga cgatgtacgg cccgtcgcgg tagtcgcgca ggtctatccg atactcgaac
     1321 cgtacgccca gcttcttgat gaacagcgcc ttccagatat tcgcgagcgc caggtagacg
     1381 atggccggcg gcctgcgtat cgtcctggcg gccagcctct cctgcaacgt tttcgtatgc
     1441 gtagtcatgg tgttcctgaa cccgtccgcc cgacggcgga cgacgatgga ctatgcgcgt
     1501 cgggcagagc cgatacggcg agccgccatt ctggacgggt cgcctcggcc tgtcaagcgc
     1561 gtcccgcgcc gcaccacgag tatcgcacga cacgccgccc cgatgctcgg cgcggcgttc
     1621 gctacccgta gttcaagcgg actcgcttcg ctcgccgctt aactacgggg ccgtacgtcg
     1681 agagtgcgag gtcgaacgac ggccggcgat cgccgcgata gtcggcgatc gcgatgttgg
     1741 actgagtcgc gggcgactcg tcagaaaatg acggaatcgt catggcgcat gcgcctgtag
     1801 tgtagaattg tttatataaa ttaagctgcg gttgcttttc ttgtcggggg gggggcatga
     1861 tggctcgtgg aaccgagtcc aagaaatcgt actgatcgtt aaggaccggt attcattcct
     1921 ttcgactgtt ttagggaggc cttttcgatg aaacgactcg taattctctc gttcgtcgct
     1981 ctcgcgtcag tgtccgcgtt cggtcaaggc ccggctggcg gcatgagcgt cgccttagcc
     2041 ggcgacagta gcatcgtata ctcgaagaac gaaatgttcg actatgttgc cctatccatc
     2101 gctccatcga tatcgatgga attctcgccc gtcggcatga tcggagccgg cgtgtctggg
     2161 gccctcgcta catccattct ttccgatagt cccggtatat tcctgttcgc catgcagacg
     2221 atcgac
//
LOCUS       DAOPHV010000473         2218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_69525_length_2218_cov_4.767453, whole genome shotgun sequence.
ACCESSION   DAOPHV010000473 DAOPHV010000000
VERSION     DAOPHV010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69525_length_2218_cov_4.767453"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLQ29_11000"
     CDS             <1..250
                     /locus_tag="PLQ29_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87216.1"
                     /translation="LEAERAELAAKVARIREKLPMAGASERSVDPDLLLAQLRMAAGQ
                     EPDDRDDDGSAKLDSDMAALGAEDALAALKRKLNEGSD"
     gene            257..1036
                     /locus_tag="PLQ29_11005"
     CDS             257..1036
                     /locus_tag="PLQ29_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPG87217.1"
                     /translation="MTRLRPLPERDRPLVFAHRGLSSEAPENTMAAFRLAKEKKAPGV
                     ELDIHLTADGKLIVIHDHTTKRVAPGTDLVVEDATAAQLQAIDIGSWKGAQFSGERPP
                     LLSELFEELGNDLYFDIEIKSGKAEDKGLEAALVKLLADFGYGAERVVISSFNPIAIK
                     RFKALCPTVPTAIIYCVDDGVPAYLRRGEGRWIGRADFLKPKHTQATKAGLAWGRMIG
                     GRPVVPWTIDDPTIAERVLRIGCDGVISNMPHRLGLPGFDR"
     gene            1179..1694
                     /locus_tag="PLQ29_11010"
     CDS             1179..1694
                     /locus_tag="PLQ29_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87218.1"
                     /translation="MNEPENPVNSGSVRQRAFNVVGWIAFLLLVPPALSMIGLPRLQE
                     LIAARLGAWGSPVALVIYFYIILFLRVFFGSDQRYAPVLMGYLTSFVYFSNSLDIGFM
                     AWLRGLTAGIPPLSYRYMSFIAGIATIFIANALSGAKKGTWIIDAILLILLPAGVLVA
                     AGVYLPGLLGL"
     gene            1691..>2218
                     /locus_tag="PLQ29_11015"
     CDS             1691..>2218
                     /locus_tag="PLQ29_11015"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG87219.1"
                     /translation="MNRAEAARASELAAEGIAKLEARRFEEAAEAFLSARRLDPASPE
                     LAFNLGLARLRDGRARDARAAFEEALALGGEDADLLCNLGLALFETGKEADAERAYLR
                     ALELEPGHPEAANDLGVLLFGRGLYDRARELFASAATKRRDYYDAWFNLRDACAELGL
                     EAERSAAQAELDRLGG"
BASE COUNT          397 a          734 c          727 g          360 t
ORIGIN      
        1 gctcgaggcg gagagggccg agctggccgc caaggtcgcg cgcatcaggg agaagctgcc
       61 gatggcaggc gcctcggagc gctcggtcga cccggacctg ctcctggccc agctacgcat
      121 ggcggccggc caggagccgg acgacaggga cgacgacggc tccgccaagc tggattcgga
      181 tatggcggcg ctcggcgcgg aggacgcgct cgccgcgttg aagagaaaac tgaacgaagg
      241 aagcgattag accgacatga ctagactgag accactaccg gagcgggaca ggccgctcgt
      301 gttcgcccat cgcgggctgt cgagcgaggc gcccgagaat accatggccg cctttaggct
      361 cgccaaggaa aagaaggcgc caggcgtcga gctggacatc cacctgacgg ccgacggcaa
      421 gcttatcgtc atccacgacc acaccaccaa gcgggtcgct cccggcaccg acctcgtcgt
      481 cgaggacgcg acggcggccc agctccaggc catcgacatc ggctcatgga agggcgccca
      541 gttctccggc gagcggccgc cgttgctctc ggagctattc gaggagctcg ggaacgacct
      601 gtacttcgac attgagatca agagcggcaa ggccgaggac aaaggactcg aggcggcgct
      661 cgtcaagcta ctagccgatt tcggctatgg ggcggagcgc gtcgtcatct cgagcttcaa
      721 tcccatcgcc atcaagcgtt tcaaggctct gtgcccgacg gtacccacgg ccatcatcta
      781 ctgcgtcgac gacggcgtgc ccgcctacct caggcgcggc gaggggcgat ggataggccg
      841 cgccgacttc ctgaaaccca agcatacgca ggcgaccaag gccgggctgg cttggggcag
      901 gatgatcggc ggccgaccgg tcgtaccgtg gacgatagac gatccgacga tagccgagcg
      961 cgtgctacgc atcggctgcg acggcgtcat ctcgaacatg ccgcaccggc tcggccttcc
     1021 cggtttcgac cgataggcat catgacctag tataggcgga gggcgcgtcg ccatacgcgc
     1081 cctccgcttt gcgtttcccc taactggttg tataatcctt accaaggttg cgcccgatcg
     1141 ccgcgaggga tcgggcgcgt cgcggaaagg aagtacccat gaacgaacca gaaaatccag
     1201 taaactccgg aagcgtcagg cagcgagcgt tcaacgtcgt cggttggata gcgttcctgc
     1261 tcctcgtgcc cccggccctg tctatgatag gcctgcctcg cctgcaggaa ctgatagccg
     1321 cgaggctcgg cgcctggggt tccccggtag ccctggtcat ctacttctac ataatactct
     1381 tcctcagggt attcttcggc tcggaccagc gctacgcgcc cgtactgatg ggctacctaa
     1441 ccagtttcgt ctatttctcc aactcgctcg acattggctt catggcctgg ctgcgcgggc
     1501 tgactgccgg aattccgccc ctctcctatc gctacatgag cttcatagcc ggcatcgcga
     1561 cgatcttcat agccaacgcg ctatccggcg ccaagaaggg cacctggatc atcgacgcga
     1621 tactcctcat actgttgccg gccggggtcc tggtcgcggc gggcgtctac ctgccgggac
     1681 tgctcgggct atgaaccgag ccgaggccgc ccgggcgtcc gagctggcgg ccgagggcat
     1741 agccaagctc gaagcgaggc gcttcgagga ggcggccgag gcttttctat cggctcggcg
     1801 gctcgacccg gcctcgcccg agctggcctt caaccttgga ctggccaggc ttcgcgacgg
     1861 gcgcgcccgc gacgccaggg ccgccttcga ggaagccctg gcgctcggcg gcgaggacgc
     1921 ggacctacta tgcaaccttg ggttggccct gttcgagaca ggcaaggaag ccgacgccga
     1981 gcgcgcatac ttgcgcgccc tcgagctgga gcccgggcac cccgaggcgg ccaacgacct
     2041 cggggtactg ttgttcggcc gcgggctgta cgatcgggcg cgcgagctgt tcgcatcggc
     2101 cgcgacgaaa aggcgcgact actacgacgc ctggttcaac ctgcgcgacg cctgcgccga
     2161 gctcgggctc gaggcggagc ggtcggccgc ccaggccgag ctcgacaggc tgggcggc
//
LOCUS       DAOPHV010000474         2215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_69636_length_2215_cov_3.750000, whole genome shotgun sequence.
ACCESSION   DAOPHV010000474 DAOPHV010000000
VERSION     DAOPHV010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69636_length_2215_cov_3.750000"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="PLQ29_11020"
     CDS             complement(<1..126)
                     /locus_tag="PLQ29_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPG87220.1"
                     /translation="MATLYMVATPIGNLEDITLRAVRTLGEVDAIACEDTRHTLRL"
     gene            189..614
                     /locus_tag="PLQ29_11025"
     CDS             189..614
                     /locus_tag="PLQ29_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87221.1"
                     /translation="MNDLVFAALLASIGAFLLWLFWALVYSPYAARKERASPRFVPDA
                     RGEMREKGVGARSCPVCGERLGPGALVKSKVYKSSGSDKVMYIYGCPYCWPENSEYRR
                     VCPVCEKVVPRDGYLLARYFERPERRHVHVLGCSGCRRG"
     gene            813..2180
                     /locus_tag="PLQ29_11030"
     CDS             813..2180
                     /locus_tag="PLQ29_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87222.1"
                     /translation="MRAISVPLVIVTAFALGPGVAAFAQGRPEGPGFEPLPLSSPAYA
                     AVDALYLATGAGTASNARPWSRSEAMQILGRIDRSSLGAQLSGLYDAAERAIGAGRRF
                     GTPDGFSLGGGLDLAAEAYYHANPDDFYLESDWAHGFESREPLARLRLDFAIDELMYV
                     YCDLQYGRNRISYLDEVSNMPDAGVGAVIGERTAVDDSADRAERGAYVERSYSFGSGL
                     ISNVVLHSYDFDFEWPKRAVATIGGDRWTASLARDSISWGNGRSGNFVLDGHADFNDY
                     ARVTAWSDRFKYDWVNVFLPTETGTAEAPDQAFRVFLGHRLEFRALDSLTLALSEDVM
                     YRNDVFDPRYLNPAFIYHNLNNRSMFNAIAHLELDWAPAPGWKLYAQYALDQGRAPNE
                     SSAQANAMAYLGGLERGVALGPGILTSSVELAWTDPLLYRRDGVDFIVVRRYFCHGDP
                     GATAT"
BASE COUNT          364 a          757 c          712 g          382 t
ORIGIN      
        1 cagcctgagc gtatgcctgg tatcctcgca ggctatcgcg tcgacctcgc cgagcgtcct
       61 gacggcgcgc agggttatgt cctcgaggtt gcctatgggg gtcgcgacca tgtacagggt
      121 tgccatcgcc ccagtatcgc cgcaatcggc tcgggcagtc aacgtcgcgc catgctatac
      181 tggcccgcat gaacgacctc gtattcgcgg ccctgctcgc gtcgatcggc gccttcctcc
      241 tctggctgtt ctgggccctc gtctacagcc cctacgccgc ccgcaaggag cgcgcctcgc
      301 ctcgcttcgt gcccgacgcc cgcggcgaga tgcgcgagaa gggcgtaggc gcgcggagct
      361 gcccggtctg cggcgagcgg ctcggccccg gcgccctggt caagtcgaaa gtctacaaga
      421 gttccggcag cgacaaggtg atgtacatct acggctgccc gtactgctgg ccggagaaca
      481 gcgagtaccg tcgagtctgc ccggtatgcg aaaaagtcgt gccgcgcgac ggctacctgc
      541 tagcgcgcta cttcgagcgc cccgagcgtc gccatgtcca cgtgctcggc tgctccggct
      601 gccgaagagg gtgatccgac tggaaccagc gaagagcata cgtgtgaagg cttgaaccac
      661 ggagacacgg agacacagag gaataagaca agttcttatc atcctctccg tgcctctgcg
      721 gcaaagccca aagagttcct tccaatccga ctatgaactc gcgggctggc ggagaggcgg
      781 cgcgagcggt agaattcgac cggaggcata cgatgcgcgc catatccgtt cctctcgtca
      841 tcgtcaccgc tttcgcgctc ggcccggggg tagcggcctt cgcccagggc cgaccggaag
      901 gtcccgggtt cgagcccctg ccgctgtcgt cgccggccta tgccgccgtg gacgcgctct
      961 acctggctac cggcgccggg accgcgtcga acgcgaggcc ctggtccagg tcggaagcca
     1021 tgcagatcct cggacggatc gaccgatcat cgctcggggc ccagctctcc gggctctacg
     1081 acgcggccga gcgggcgata ggcgctggac ggcgcttcgg tacgcctgac gggttcagcc
     1141 tggggggcgg gctcgacctg gcggccgagg cctactacca cgcgaatccg gacgacttct
     1201 atctcgagtc cgactgggcc cacggcttcg agtcgaggga gccgctggcc aggctcaggc
     1261 tcgacttcgc catcgacgaa ctcatgtacg tctactgcga cctgcagtac ggccggaacc
     1321 ggatcagcta cctggacgag gtatccaaca tgcccgatgc cggcgtcggc gccgtgatcg
     1381 gcgagaggac ggccgtggac gactcggcgg atcgagcgga gcgaggcgcc tacgttgagc
     1441 gttcgtatag tttcggtagc ggcctgatct cgaacgtcgt tctccatagc tacgacttcg
     1501 acttcgagtg gcccaagcgg gccgtggcga cgataggcgg cgatcgatgg accgcgtcgc
     1561 tcgcgaggga ctcgatatcg tggggaaacg gccgttccgg caacttcgtg ctcgacggcc
     1621 acgccgactt caacgactac gcgcgcgtca ccgcttggtc cgaccgcttc aagtacgact
     1681 gggtcaacgt gttcctgccg acggagacgg gtacggccga ggccccggac caagcgttcc
     1741 gggtattctt ggggcaccgg ctcgaattca gggcgctgga tagcctgacg ctcgcgctgt
     1801 ccgaggacgt gatgtaccgt aacgacgtct tcgacccgcg ctacctgaac cccgccttca
     1861 tctaccacaa cctgaacaat cgctcgatgt tcaacgccat cgctcacctg gagctggact
     1921 gggcccccgc gcccggctgg aagctctacg cccagtacgc cctcgaccag ggccgcgctc
     1981 cgaacgagtc gtcggcccag gccaacgcca tggcctacct cggcggcctc gagcggggcg
     2041 tcgcgctcgg cccgggcata ctgacctcgt ccgtcgagct ggcctggacc gatcccctgc
     2101 tctaccgccg cgacggcgtc gacttcatcg tcgtccgcag gtacttctgc cacggcgatc
     2161 cgggggctac ggctacgtga gccacctcga ctacctgggc tatccgtacg gcggc
//
LOCUS       DAOPHV010000475         2210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_69853_length_2210_cov_3.641763, whole genome shotgun sequence.
ACCESSION   DAOPHV010000475 DAOPHV010000000
VERSION     DAOPHV010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69853_length_2210_cov_3.641763"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="PLQ29_11035"
     CDS             complement(<1..121)
                     /locus_tag="PLQ29_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPG87223.1"
                     /translation="MADNKNLRTNEQIRVREVRLIDDAGEQKGVLQTADALRMA"
     gene            296..697
                     /locus_tag="PLQ29_11040"
     CDS             296..697
                     /locus_tag="PLQ29_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87224.1"
                     /translation="MIENVQEEPSNLSAVCADAVIGYREAGKAVQAPAIRKVIESVLR
                     QKIEHVSALRPLDALFSGSLERLSIPSGAEPEDVLRHLVSHETGFAASLDSFSASLDD
                     LEARQSVKGIADCSRKFASWAQDHLDLLAMF"
     gene            complement(1133..1285)
                     /locus_tag="PLQ29_11045"
     CDS             complement(1133..1285)
                     /locus_tag="PLQ29_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87225.1"
                     /translation="MKKKAVPNAATTQPSVARASGLSAPGMPSLTASSNRYGKARAVA
                     TSIKGR"
     gene            complement(1398..1471)
                     /locus_tag="PLQ29_11050"
     tRNA            complement(1398..1471)
                     /locus_tag="PLQ29_11050"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1435..1437),aa:Arg,seq:tct)
     gene            complement(1477..1549)
                     /locus_tag="PLQ29_11055"
     tRNA            complement(1477..1549)
                     /locus_tag="PLQ29_11055"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1514..1516),aa:Pro,seq:tgg)
     gene            1784..>2210
                     /locus_tag="PLQ29_11060"
     CDS             1784..>2210
                     /locus_tag="PLQ29_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPG87226.1"
                     /translation="MAYTTDLIRNVALAGHGSTGKTSLLEHILFSAGAIQKPELLESG
                     KTVSDYAEEEIERKISVHAALAHAIVGDVKINLLDTPGSSDFIGDVILALRSCESAVV
                     LLGARAGVQIETIKIWRNLEGRAKPRMLFVNKLDEDRASF"
BASE COUNT          375 a          690 c          666 g          479 t
ORIGIN      
        1 tggccatcct gagggcatca gccgtctgaa ggacgccctt ctgttctccg gcatcgtcga
       61 ttaaccgtac ttcacggacc ctgatctgtt cgttggtccg cagattctta ttgtccgcca
      121 atggacctcc ttcggcttac cctgcaaata tctatagtgc tgaacattgt agcatagagc
      181 cactggtcga gtcaaagggt gcgccgcttc agcgtagggt ggacgctatc gcgaatgtaa
      241 gaagctcgta agctacgcat ttgacagcca tagttcagcg tagtatcgtt acactatgat
      301 cgaaaatgtt caagaggagc cttcgaacct gtccgcagtc tgcgcggatg ccgtcatagg
      361 ctatcgcgag gctggaaagg ccgttcaggc acccgccata cgcaaggtta tcgaatcggt
      421 gctcaggcag aagatcgagc atgtgtcggc ccttcgaccc ttggacgccc tgttctccgg
      481 ctcgctcgaa cgcttgtcga taccctccgg ggccgagccc gaggacgtgc ttcgacacct
      541 ggtctcccac gagacgggct tcgcggcatc gctcgattcg ttttccgcct cgctcgacga
      601 cctggaagct aggcaatcgg tcaagggcat cgccgattgt agcaggaaat tcgctagctg
      661 ggcccaggat cacctcgacc tactcgcgat gttctagcgc ccttccggcg acccttgcct
      721 cgcgggcgtc ggcgtggtag ccttcgcgca tgtacgtttt cgccatgctt tcgatgcctc
      781 tctcgttcat ggccgtctcc gcctggactt tcagcgtcgg cgacaagagc ggtcgcgcct
      841 tcgcgcgcgg gctcctgttc ggcctgcccg cggccctcgt ctgggtcctc gttcgccggc
      901 tgctgatgcc cgtatggggt tctcccgtcc tggccatcat cttcttcgct aggtattggg
      961 cgctgccgct cggtttgacg acgctagcct acgcggcggc ggtcggcttc tcgagcgtcg
     1021 ctcgcgacgg agagtacgac cgcctcgtat cgttcttcat gggctcatta tcggtcttcg
     1081 gcgtcgccca cgccatatcg tcatgggggg gccggagcgc cgtattctcc atctacctac
     1141 ccttgatgct cgtcgccacg gccctggcct ttccgtacct gttggaggaa gcggtcaagg
     1201 acggcatgcc aggcgcgctc aagccgctgg ccctggcgac gctcggttgc gtcgtagcgg
     1261 cgttcgggac ggccttcttt ttcatgaggc tggaatggct ggggtacctg acgtcggcgc
     1321 tgtacacggc cggcgccacg gcgctcggtc tatccaggct actgcgagct ccccacggcg
     1381 ctcggcgcgg cgaggcatgg gaccagcggg gctcgaaccc accacctacg gcttagaagg
     1441 ccgttgctct atccagatga gctatgatcc cggaggtcgg gacgggggga ttcgaacccc
     1501 cgatatccag ctcccaaagc tggcgcctta gccgctaggc tacgtcccgg tcaagggtat
     1561 tgtataagcc gcgctccgat cgtttgtcaa tgagcgccgc tatccttcgc gccgaggccg
     1621 cccggcccgc ggcctcggcg cgagcctgtt tttcttgaca actcctggaa taggtttcat
     1681 cattcgagga cttagtacga aacggttcta ctgttccgtc gggggctcgg ccgcgtcgaa
     1741 cgacgtcgcg aagggcctcc aaccttcctt aaggagcgag tctatggcct acacgaccga
     1801 cctcatcagg aacgtggccc tagcgggcca cggtagcacg ggtaagacga gcctgctcga
     1861 acatatcctc ttcagcgccg gcgccatcca gaagccggaa ttgctcgaat ccggtaagac
     1921 ggtgagcgat tacgctgagg aggagatcga gcgaaagatt tcagtacacg cggcgctcgc
     1981 gcacgcgatc gtcggagacg taaagatcaa cctgctggat acccccggat cgtccgattt
     2041 catcggggac gtgatcctgg ccttgaggtc ctgcgagtcg gccgtcgtat tgctcggcgc
     2101 cagggccggc gtgcagatcg agaccatcaa gatctggcgc aatcttgagg gccgtgccaa
     2161 gccgcggatg ctgttcgtca ataagctgga cgaggatagg gcctcgttct
//
LOCUS       DAOPHV010000476         2203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_70160_length_2203_cov_4.604749, whole genome shotgun sequence.
ACCESSION   DAOPHV010000476 DAOPHV010000000
VERSION     DAOPHV010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70160_length_2203_cov_4.604749"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1215
                     /locus_tag="PLQ29_11065"
     CDS             76..1215
                     /locus_tag="PLQ29_11065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPG87227.1"
                     /translation="MLEDRHGGAATSPDVVSQYNRLNQLGAIDNLEALRLENRELDRL
                     VTDAAALVALPSVPLMLDFVIDKALERFVPTFLAFVIEPPRGNRLSQFCYRNLKPSDE
                     TIPLDCYRRIKSRFLSKPFTERFDTILAETGEYPELEAFSPELVFPMRGIDGLYGIVV
                     LGEKVVGDEYSEVETMYLDRLTRFLAVGLQNGLHHQSSITDAKTGLYNHEFFMRRLAE
                     EQARCSRSGAQACILMLDVDRFKRFNDSHGHLAGDAALEALAATLKSTMRTGDAVGRF
                     GGEEFCVLALDCDARGAMDAAERVRRSVESLRFSYDGQELSITVSLGIRMLDPGIDPK
                     DLLDDADKALYASKSGGRNRSTLFRPGLLGRAELLRAADPNLCRP"
     gene            1212..2036
                     /gene="yqeC"
                     /locus_tag="PLQ29_11070"
     CDS             1212..2036
                     /gene="yqeC"
                     /locus_tag="PLQ29_11070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HPG87228.1"
                     /translation="MNCLSLVAGAPRTTGRPMTVAIVGGGGKTSLLFSLASDTSAIGL
                     SALVTTTTRIFDPRDEGRRFDELVLDAGLAAEASRPRPLPSPEAWQRRSVASGRGPVV
                     VAAAGVDGGRLLSVDPARVDEARGWDLVAVEADGARGLPLKAPADHEPVVPAGSGVVI
                     AVIGLDCLGRRIDDTIAFRPALVAKAAGVAEGETIRPQHLAALAASPAGSFKSAPPGS
                     LRVVVLNKLDLVERTVADEAAEVILAVGAADIVVLACLADPDPSRRVVSAIGYRTV"
     gene            2047..>2203
                     /locus_tag="PLQ29_11075"
     CDS             2047..>2203
                     /locus_tag="PLQ29_11075"
                     /inference="COORDINATES: protein motif:HMM:NF014662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HPG87229.1"
                     /translation="MELFSKAAELTAANIPFAMATIVEASGSTPRGKAKMLVTASGVV
                     AGTVGGGA"
BASE COUNT          361 a          736 c          732 g          374 t
ORIGIN      
        1 tataatgata cgcagtataa aaagcaaggc tccgacccgt atccatcgaa ggcggacatg
       61 tattagtatt gcttcatgct cgaggatagg cacggcggag ccgctacttc ccctgacgtt
      121 gtatcgcaat acaatcgact caaccagctc ggcgccatcg ataatctcga ggccctccgc
      181 ctcgagaacc gcgagctgga tcgcctcgtc accgacgcgg cggccctcgt cgccctgcct
      241 tcggtcccac tgatgctcga cttcgtgatc gacaaggcgc tcgagcgctt cgtgccgacc
      301 ttcctggcct tcgtcatcga gcctcctcgc gggaacaggc tgagccagtt ctgttatagg
      361 aatctcaagc cgagcgatga gacgatcccc ctcgattgct accgccgcat caagagccgc
      421 ttcctgtcca agcccttcac cgagcgcttc gacaccatcc tggcggagac cggcgagtac
      481 cccgagctcg aggccttctc accggagctg gtgttcccga tgcgcggcat cgatggcctc
      541 tatggcatcg tcgtgctcgg ggaaaaagtc gtaggcgacg agtactccga ggtggagacg
      601 atgtacctgg atcggctgac gcgcttcctg gcggtcggcc tccagaatgg gctccaccac
      661 cagagttcga tcaccgacgc caagaccggg ctctacaatc acgagttctt catgcgcagg
      721 ctcgccgagg agcaggcgcg ctgttcccgc agcggcgctc aggcctgcat cctgatgctc
      781 gacgtggatc gctttaaacg cttcaacgac agccacggcc acctggccgg cgacgccgcc
      841 ctcgaagccc tcgccgcgac gctcaagtcc acgatgcgta ccggcgatgc cgtagggcgc
      901 ttcgggggcg aggagttctg cgtgctggcc ctggactgcg acgctcgcgg ggccatggac
      961 gcggccgagc gcgtcaggcg atcggtggag tcgctgcgct tctcgtacga cggccaggaa
     1021 ctctcgataa ccgtgagcct cgggatcagg atgctcgacc ccgggatcga cccgaaggac
     1081 ctgctcgacg acgccgacaa ggcgctctac gcctcgaagt ccggaggccg caaccgctcc
     1141 accctgttca ggccggggct actcggaagg gccgagctcc tgcgggcggc cgacccgaat
     1201 ctctgccgtc catgaattgc ctgagcctcg tcgcgggcgc gccgcgtacg accgggcgcc
     1261 cgatgacggt cgccatcgtg ggcggaggcg gcaagacgag cctcttgttc tccctggctt
     1321 cggacacgtc ggccatcggc ctatcggccc tggtcacgac gacgacgcgc atcttcgatc
     1381 cgcgcgacga gggccggcgt ttcgacgagc tggttctcga cgcgggcttg gcagcggaag
     1441 cgtcgcgccc gcggcctctg ccgagtcccg aggcctggca gaggcgaagc gtggcgtcgg
     1501 ggcgtggccc cgtggtcgtg gcggctgccg gcgtcgacgg cggcaggcta ctgtccgtcg
     1561 acccggcccg cgtcgacgag gctcgaggct gggacttggt cgcggtcgag gcggacggcg
     1621 ccaggggact accgctcaag gctccggctg accacgagcc ggtcgtgcca gctggctcgg
     1681 gcgtcgtgat agccgtgata ggcctcgatt gcctcggacg ccgcatcgac gatacgatag
     1741 ccttcaggcc cgccctcgtg gcgaaggccg ccggggtggc cgagggcgag acgatacggc
     1801 cgcaacacct cgcggccctg gcggcgagtc cggctggctc tttcaagtcg gcgccgccgg
     1861 ggagcctccg agtcgtcgtc ctcaataagc tggatctggt cgaacgaacc gtggccgacg
     1921 aagcggccga ggtcatcctc gcggtcggcg cggccgatat agtcgtgctg gcgtgcctcg
     1981 cggaccccga tccgtcgcgg cgcgtcgttt cggcgatcgg gtatagaaca gtataaagga
     2041 gcgatcatgg agctattctc caaggcagcc gagttgaccg ccgcgaacat acccttcgcc
     2101 atggcgacga tagtcgaggc ttccggttcg acgccgcgcg gcaaggccaa gatgttggtc
     2161 acggcctccg gcgttgtggc ggggacggtc ggcggcggcg cgg
//
LOCUS       DAOPHV010000477         2203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_70172_length_2203_cov_2.989758, whole genome shotgun sequence.
ACCESSION   DAOPHV010000477 DAOPHV010000000
VERSION     DAOPHV010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70172_length_2203_cov_2.989758"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1969)
                     /locus_tag="PLQ29_11080"
     CDS             complement(<1..1969)
                     /locus_tag="PLQ29_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HPG87230.1"
                     /translation="MHGDHLLGQLESIGPEMRSAVLSQERAGLTEDRGIRIEFESYPG
                     FPNAFEKLTYGTTGIELLNVRRSGERSYATVFVPDGKLSYFIKQMQKYVANATSDGKP
                     VKDQHELIDTIQNIKLASIRALWTDADEAFPRDSEEPFWWEAWLSVRGDRKRAVREFR
                     DMAAFYRMSTSSATYDFPERTIVLVRSNLSTMLGALPILTTVAELRRAKDTAAFFADL
                     KPYEERPWVDDLASRVTHADTVDSVPRVCVLDTGVTRGHPLLEGAIEATDMSTIRPDW
                     GTADSDGHGTGIAGLALYGDLTYPLASTSPLVLEHRLESSKLLAEDGGNAGDQHLHAK
                     LTMQAVYSPLIDNPGRRRVYELAISADDYRDKGRPSAWSAAIDRMASAATLEGEDPLE
                     PSLFVIAAGNNRDSATWIRYPTCVDGELIHDPGQSWNALTVGAYTDKVDIAGDKTRGY
                     TPIARAGDLSPFTSTSSEWDEPCPIKPDVVMEGGNAGLDAYSAVTVDSLSLLTTNNEP
                     SVRLLKAFNATSAASALAARMAARIMAAYPALRPETVRGIIVHSARWTDAMLERYGMN
                     GHSNKGEYLDLVRRCGFGVPDEDSALWSLSNSVTMLIEDSIAPFKKVGSTITTKEAVY
                     YDLPWPVEVLEDLGSTDVELRVTLSYFIEPNP"
BASE COUNT          439 a          679 c          604 g          481 t
ORIGIN      
        1 aggggttggg ttcgatgaag taggatagtg taacgcggag ctcaacgtcc gtggaaccta
       61 aatcttctag gacctcgacg ggccacggta gatcgtagta cacggcctcc ttcgtcgtta
      121 ttgtactacc gacctttttg aatggggcaa tggagtcctc gataagcatc gtaacggagt
      181 tcgataggct ccacaaagcg ctatcctcgt cggggacgcc gaagccgcat cgccgtacca
      241 gatcgaggta ctcacccttg ttactatgac cgttcatgcc ataccgctct agcatcgcgt
      301 cggtccatcg ggccgagtgt acgatgatac cgcgaaccgt ctcgggcctc agcgctgggt
      361 acgcggccat gatcctcgcg gccatcctcg cggcgagcgc cgacgccgcg ctcgtcgcgt
      421 tgaaggcctt gaggagacgg acggacggct cgttattcgt cgtaaggagg ctcaacgaat
      481 cgacggtcac ggcgctgtaa gcgtccaagc cggcgttccc gccctccatg accacatcgg
      541 gcttaatcgg gcacggctcg tcccactccg aggacgtcga tgtaaacggg ctcaagtccc
      601 cggcccgcgc tatcggtgta taacccctgg ttttatcgcc cgctatatcc accttatcgg
      661 tatacgcccc aacggtgagg gcgttccatg actgaccagg atcatgtatg agctcgccat
      721 cgacgcatgt cggatagcgt atccatgtcg cagaatctcg gttatttccg gcggcgatga
      781 cgaacaggct cggttcgagc gggtcttctc cttccagagt tgccgctgac gccatccggt
      841 ctatggccgc cgaccatgcc gagggcctgc ccttgtccct atagtcgtcg gcgctaatgg
      901 ctagctcgta gacgcgccgc ctgccaggat tatcaattaa cggagaatat acagcctgca
      961 tcgtaagctt cgcgtgtaga tgctggtccc cggcgttacc gccgtcctcc gcgagtagct
     1021 tgctcgattc gagtcgatgt tcgagtacca acggagaagt agaagctagc ggatacgtta
     1081 aatccccata aagagccaag ccggcgattc ccgtgccatg tccgtccgag tcagccgtac
     1141 cccagtcagg ccgtatcgtc gacatatcag tagcttctat cgccccctcc aacagaggat
     1201 gtcccctagt aaccccggta tcgagcacgc acacgcgagg aacggaatcc accgtgtctg
     1261 cgtgagtaac cctcgacgcg aggtcgtcga cccagggccg ttcctcgtac ggcttgaggt
     1321 cagcaaagaa cgcggcggtg tccttggctc gacgcagctc ggctaccgtg gtcagaatcg
     1381 gcaacgcgcc gagcatggta ctgagattag atcgtacgag cacgatagtc cgttcaggga
     1441 agtcgtacgt tgcgcttgac gtactcatgc ggtagaaggc ggccatatct ctgaactctc
     1501 gaaccgcgcg cttacggtcg cctcggaccg aaagccacgc ctcccaccag aacggttctt
     1561 ccgaatctct cgggaatgcc tcgtcggcgt cggtccatag cgccctgatg ctcgccagct
     1621 tgatgttctg tatcgtgtcg atcaactcat gttgatcctt tacaggcttt ccatcactcg
     1681 ttgcattcgc cacatacttc tgcatttgct tgatgaaata gcttagcttc ccgtcaggca
     1741 caaacacggt ggcataggac ctttcgccag accgccgcac attgagtaac tcgattcccg
     1801 tagtcccata cgtcaatttc tcgaaggcat tagggaaacc gggatagctc tcgaactcga
     1861 tgcgtatgcc gcgatcctct gtcaggcctg cccgttcctg gctcagtacc gccgatcgca
     1921 tctcagggcc gatagactcc agctgcccga ggagatggtc gccatgcacg gctcggtcgc
     1981 gagcttgtat tgctcggtct cggccattag cgacgatagt gaacgggtag cgcgtaccac
     2041 gaccatgaac gacgaggtgc ctatgccgag catcgtctct atcgggcatg cgcgcgctcc
     2101 ttacttaaat cgtttcaacc tctccacgac atcgtgacgc tcgagaatag cctgtccgag
     2161 gcgctcctcg gatatacgat cctgcccgtc tatcagggcg tcc
//
LOCUS       DAOPHV010000478         2194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_70545_length_2194_cov_3.640954, whole genome shotgun sequence.
ACCESSION   DAOPHV010000478 DAOPHV010000000
VERSION     DAOPHV010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70545_length_2194_cov_3.640954"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLQ29_11085"
     CDS             complement(<1..346)
                     /locus_tag="PLQ29_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG87231.1"
                     /translation="MGSPFILIIVYLAFISLGLPDSTLGVAWPTFRLAVGAPLEAAGL
                     VAVVLTACSAVSSLASGHVTRRFGTGRVVAVSCTLTALGLAGYSVAPSFAWVIAAAVP
                     LGFGQGAVDSSLN"
     gene            complement(349..1386)
                     /locus_tag="PLQ29_11090"
     CDS             complement(349..1386)
                     /locus_tag="PLQ29_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPG87232.1"
                     /translation="MYAGRPERMKQVNEALVREALAARGKATKAQLGADTGLSLPTIS
                     QATAALTERGEIRAAGMMRSSGGRKATEYELDEAAGTVYAIALERDRIDWAMANALGT
                     VVSTGGRLVRSNPVSEAVELVGAMRRDAVIRSSERVVLAIGVPGAVQDGRVITGHFEE
                     QWYDTDIAEFFRKRTGLPVVVENDINASALGYERRLDSIGQCPHSLVYVYVSGTCTGS
                     GIVVGGQVVRGAAHFAGELDYLPVARGQTLRDALAGAVGDESIVDALASALAAVNCVV
                     NPALFVVGGPGFRFDLSDAVEAEFKARVPVSVRPRLAFERDTMPHYLNGLCGLALDRM
                     FSSFRLVSGGT"
     gene            complement(1474..1980)
                     /locus_tag="PLQ29_11095"
     CDS             complement(1474..1980)
                     /locus_tag="PLQ29_11095"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPG87233.1"
                     /translation="MDQAYLRLLEESKERNAFIKRQLKYLGKFNTKNFDHVVADFSSE
                     VFEGIDCLRCGNCCRTIGPKMSESDIKRACKAYGLDQREFTETSLRRDEELGWVCVSM
                     PCPFLRDDDGCAIYDDRPHDCADFPYMEERGIQRSLGRLAFNSEFCPAAYLIAMKIIE
                     KYAPGVTK"
BASE COUNT          410 a          721 c          669 g          394 t
ORIGIN      
        1 cgttgagcga cgagtcgacc gccccctgcc cgaagccgag cggtacggcc gcggctatca
       61 cccaagcgaa tgacggggcg accgaatagc cggccaggcc gagggctgtc agagtgcagc
      121 taacggcgac gacgcggccg gtgccgaagc gccgggtcac gtgcccgctg gctaggctcg
      181 atacggccga gcaggccgtc aacacgacgg ctacgaggcc ggccgcctcg agcggggcgc
      241 cgacggccag cctgaaggtc ggccacgcga cgccgagcgt cgagtcaggc aggccgaggc
      301 ttatgaacgc caggtagacg atgatcagaa tgaacggcga tcccatggtc aagtcccgcc
      361 cgataccagc ctgaacgagc tgaacatacg atccagcgcc aggccacaca ggccattcag
      421 gtaatgaggc atcgtatccc gctcgaacgc caggcgaggc ctgaccgaga ccggtacccg
      481 agccttgaat tccgcctcca cggcgtccga taggtcgaag cggaagcccg gcccgccgac
      541 gacgaataag gccgggttga cgacgcagtt gaccgcggcc agcgccgacg ccaaggcgtc
      601 gacgatgctt tcgtcgccga ccgcccctgc caacgcgtcc cgtagcgttt ggccgcgggc
      661 gacgggcagg taatcgagct cgccggcgaa atgcgcggcc ccgcgcacta cctggccacc
      721 gaccacgatg cccgaaccgg tacaggtccc cgaaacgtac acatagacga ggctgtgcgg
      781 gcattgtccg atggaatcca agcgccgctc gtagccgagc gcggaggcgt tgatgtcgtt
      841 ctcgacgaca accgggagcc cggtgcgctt ccggaagaac tcggctatat cggtatcgta
      901 ccactgctcc tcgaagtgcc cggtgatcac tctgccgtcc tggaccgcgc ctggtacgcc
      961 aatggctagc acgacgcgtt ccgacgaccg tatgacggcg tcgcgcctca tcgcgccgac
     1021 gagctcgacc gcttcgctca cgggattcga tcgcaccagg cgccctcccg tggagacgac
     1081 ggtgccgagg gcgttggcca tggcccagtc gatgcgatcg cgttcgaggg ctatcgcgta
     1141 gacggtgccg gccgcctcat ccagctcata ctcggtagcc ttgcgccccc cgctcgaacg
     1201 catcatcccc gctgcgcgta tctctccgcg ctcagtcagc gccgccgtgg cctgggagat
     1261 cgtcggtagg cttaagcccg tgtcggcgcc gagctgggcc ttcgtcgcct taccgcgcgc
     1321 ggctagggct tcgcgtacca atgcctcgtt cacctgcttc atccgctcgg gccttccagc
     1381 gtacatcgac actccattat cttattaagc ttactttatt aagtatcctt acttgatgaa
     1441 ttgtcaatag acctccgcta catagagcgt ccgctacttg gtcaccccag gcgcatactt
     1501 ctcgatgatc ttcatggcta tcaggtaggc cgccggacag aactccgaat tgaaggccaa
     1561 tcggccgagc gaacgctgga tgccacgctc ttccatgtac gggaagtcgg cgcagtcatg
     1621 tggccggtca tcgtagatgg cgcagccgtc gtcgtcgcgc aggaaggggc acggcatcga
     1681 tacgcatacc cagccgagct cctcgtcgcg cctgagcgac gtctcggtga actcgcgctg
     1741 gtcgagcccg taggccttgc aggcccgctt gatatcgctc tcgctcatct tcgggccgat
     1801 ggtcctacaa cagttgccgc agcggaggca atcgatcccc tcgaagacct cggagctgaa
     1861 atcggccacc acgtggtcga agttcttggt attgaactta cccaggtact tgagctggcg
     1921 cttgatgaac gcgttacgct ctttgctctc ctcgagcagg cggaggtatg cttgatccat
     1981 acggatagtc tacgccgaaa aggctcgcgg actcaagccg aggctcggcg caccgttgga
     2041 agcggtatgg acttgccccg ctttcgtgga gagctagtta agcaactctc ctgggtaccg
     2101 ccgcgggtgg cctgaaggca ttccgcgagt ggatcctgat ccgattataa tatgcctcga
     2161 tgtattcgaa cacggccatt ctgaccttat tgcg
//
LOCUS       DAOPHV010000479         2185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_70934_length_2185_cov_5.149765, whole genome shotgun sequence.
ACCESSION   DAOPHV010000479 DAOPHV010000000
VERSION     DAOPHV010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70934_length_2185_cov_5.149765"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2185
                     /locus_tag="PLQ29_11100"
     CDS             <1..>2185
                     /locus_tag="PLQ29_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HPG87234.1"
                     /translation="SYEDDVHANTCPITQSLPYYIAKAFPEIDEQKFLPLVVSFKFGD
                     KKALELFCTMTARLGIGEAETKAAFDKALAKQREYFAAAAALGREALEEAKAAERPVI
                     AVLGRPYNAFTPEANMGIPRKFTTRGYSVIPFDILPLEETDNRFPNMYWYYGQQDMRA
                     AQVLKDNPNIYVTWITNFSCAPDSFMLHYLKWTMGQKPFLVLELDSHSADAGIDTRVE
                     AFLDILDGYRAKKAEIEAERYDNGWKFKYGDGEDLRVVNSKTGEKVMLRDNKRAKILL
                     SNMGNVSTEFMAATIRGLGLNAEALPVATNKTIQVARAHASGKECVPSHLLLGSVLQY
                     LASEKYRKDELYLVFVPITTGPCRTGQYFVFYENLFRDLKLENVVVFTLSADNSYNEL
                     GPSFAKEMWKGLVVTDYMKDIQNALLTTAEDPEKAVHAFEDSWKGMMETVERRPADLW
                     KELRRVAGDVKRIPLKRNLADCPKVLVVGEIYVRRDDFAVGELTRLMSDRGIAVKVSG
                     IGEWIHYLDFVREYALKKLIRLQPAGTRLASKPSRDLLKLRIEEWWKHSIEKKVLSIL
                     EPTGLIPRTPHDMREIMKNTQEHFVNLELNSEIAVSSGVAATAMMSGGYSGIVNISPF
                     ACLIGRVIEGLFAPWARDRNYPTISVEVDGNQLPPNIVNKLNIFMVNVLRFESDRELS
                     SLIEPANNPLGSTLKLEQFHDDVAPSSACAHCSVRTDDDECLGSCG"
BASE COUNT          480 a          716 c          598 g          391 t
ORIGIN      
        1 tcctacgagg acgacgtgca cgcgaacacc tgccccatca cgcagagcct cccgtactat
       61 atagcaaagg ccttccccga gatcgacgag cagaagttcc taccgctcgt cgtcagcttc
      121 aagttcggcg acaagaaggc gctcgaacta ttctgcacca tgacggcgcg cctgggcatc
      181 ggcgaggccg agacgaaggc cgccttcgat aaggcgctcg ccaagcagcg cgaatacttc
      241 gccgcggccg ccgcccttgg acgcgaggcc ctcgaggagg ccaaggccgc ggagcgcccg
      301 gtcatcgccg tactcggccg cccgtacaac gcgttcaccc ccgaagcgaa catgggtatt
      361 ccgaggaagt tcaccacacg cggctactcc gtcataccct tcgatatcct cccgctcgag
      421 gagaccgata accgcttccc gaacatgtac tggtactacg gccagcagga catgcgcgcc
      481 gcgcaggtac tcaaggacaa cccgaacatc tacgtgacct ggatcacgaa tttctcctgc
      541 gcgcccgact cgttcatgtt gcactacctc aaatggacca tgggccagaa gcccttcctt
      601 gtactcgaac tggattccca ctccgctgac gccggcatcg atactcgcgt cgaggccttc
      661 ctcgacatcc tggacggcta ccgagccaag aaggcagaga tcgaggcgga acgctacgac
      721 aatggctgga aattcaagta cggcgacggc gaggacctac gcgtcgtcaa ctcaaagacc
      781 ggcgagaagg tcatgctccg cgataataag cgagccaaga tactgctatc gaacatgggc
      841 aacgtctcga ccgagttcat ggccgcgacg atccgcgggc tcgggctcaa cgccgaagcc
      901 ctgccggtcg ccacgaacaa gacgatccag gtagcccgcg cccacgcctc cggcaaggaa
      961 tgcgtgccga gccacctgct actcggcagc gtgctccagt acctagcttc ggagaaatac
     1021 cgtaaggacg aactgtacct cgtcttcgta ccgatcacga ctggtccctg caggactggc
     1081 cagtacttcg tattctacga gaatctcttc cgagacctca agctcgagaa tgtcgtcgta
     1141 ttcacgctat ctgcggataa ctcctataac gagctcgggc cgagcttcgc caaggagatg
     1201 tggaagggcc tcgtcgtcac cgactacatg aaggacatcc agaatgccct gctgactacc
     1261 gccgaggatc ccgagaaggc ggttcatgcc ttcgaggact cctggaaggg catgatggag
     1321 accgtggaac gtaggccagc cgacctctgg aaggagctca ggcgcgtcgc cggagacgtc
     1381 aagcgtatcc cgctcaagcg caacctcgcc gactgcccga aggtgctcgt cgtcggggag
     1441 atctacgtgc gccgcgacga tttcgcggtc ggcgagctca cgaggctgat gagcgaccgc
     1501 ggtatcgccg tcaaggtctc cggcatcggc gagtggattc actacctcga cttcgtgcgc
     1561 gagtacgcac tcaagaagct catccgactc cagccggccg gtacgcgtct tgcctccaaa
     1621 ccgtcacgcg acttgctcaa gctccgcatc gaggaatggt ggaagcactc gatcgagaag
     1681 aaggttctgt ccattctcga accgacgggc ctcatcccga gaacgccgca cgacatgcgc
     1741 gagattatga agaacaccca ggaacacttc gtcaacctcg agctcaactc ggagatcgcc
     1801 gtatcctcgg gagtcgccgc caccgccatg atgagcggcg gctattctgg tattgtcaac
     1861 atctcgccgt tcgcatgcct gatcggccgc gttatcgagg gactcttcgc cccttgggcg
     1921 cgcgaccgca actatcctac catctcggtc gaggtcgacg gcaaccagct tccgccgaac
     1981 atcgtcaaca agctcaacat tttcatggtc aacgtgctcc gcttcgagag cgacagagaa
     2041 ctgtcgagcc ttatagagcc cgcgaataat cccctcggct ctacattgaa gctcgaacaa
     2101 ttccatgacg acgtcgcccc atcatccgct tgcgctcatt gctcagttcg aactgacgac
     2161 gatgagtgcc tcggatcttg cggca
//
LOCUS       DAOPHV010000480         2185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_70973_length_2185_cov_2.269953, whole genome shotgun sequence.
ACCESSION   DAOPHV010000480 DAOPHV010000000
VERSION     DAOPHV010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70973_length_2185_cov_2.269953"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2185)
                     /locus_tag="PLQ29_11105"
     CDS             complement(<1..>2185)
                     /locus_tag="PLQ29_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPG87235.1"
                     /translation="VETLIWLTEVAPTSGKTGSAFIEHFGAASEAANPGLFRLALKLA
                     TGTGKTTVMAMIIAWQTINAVRHPQSKRFTKGFLIISPGITIRDRLQVLRPNDPNSYY
                     ASRELVPTELLGELSKAKIVITNYHAFMLRERVTLSKGGRSLLKGRGPDLQTLETEGQ
                     MLQRVMPELMGLKDILVINDEAHHCYREKPKGIDDEEETIGSDEKDEAKENAEAARVW
                     ISGIEAVQDKLGVTRVVDLSATPFFLRGSGYAEGTLFPWTASDFSLMDAIECGIVKLP
                     RVPVADNIAGGDMPKYRKLWDLVGPKMPKKGRGKSGSLDPLSIPVELQTALDALYGHY
                     EKTFNLWQEAGLDSPPCFIIVCNNTASSKLVFDYISGFRRTNGDGSERFENGRLPLFR
                     NFAEDGSALSRPRTLLIDSQQLESGGKLDDSFLAAASEELSLFRREIIERTGDRKKAD
                     EISDQDILREVMNTVGKPGHLGESIRCVVSVSMLTEGWDANTVTHVLGVRAFGTQLLC
                     EQVIGRALRRQSYDLNGDGLFDVEYADVLGIPFDFTAKPVVAPPKKPEKLIHVHAVRP
                     ERDDLEIRFPRVMGYRVDLPEERLSARFGPDSTLVLTPDLVGPTKVSNSGIIGQAVDL
                     SLERLESTRRTTILYHITKRLIETKFRDPSEEPKLYLFGQLKRITAEWLEHHLECRGG
                     TYPAQLLYQELADMACERITQAVSSAFEGERPIMAILDPYNPEGST"
BASE COUNT          385 a          653 c          673 g          474 t
ORIGIN      
        1 gtcgagcctt cgggattata cgggtcgagt atcgccatga tgggtcgctc tccctcgaag
       61 gcgctcgaca cggcctgcgt aatgcgctcg caggccatat cggctagctc ctggtagagg
      121 agctgggcgg ggtacgtgcc gcccctgcat tccaggtgat gctctagcca ttcagccgtg
      181 atacgcttga gctggccgaa gagatagagc ttgggctctt ctgaaggatc cctgaacttc
      241 gtctcgatga ggcgtttcgt aatatggtac aggatcgttg tacggcgggt cgactcgagg
      301 cgctctaggc tcaggtccac ggcttggccg atgatgcccg aattgcttac cttcgtaggc
      361 ccgactagat cgggggtcag gaccagcgtc gagtcaggcc cgaaccttgc gctcagccgt
      421 tcctcgggca gatccacccg gtaacccatg acgcggggaa aacgtatctc caggtcatcg
      481 cgctccggcc gaaccgcgtg tacgtgtatg agtttctccg gcttcttggg aggcgctacg
      541 accggcttag ccgtaaagtc gaaggggatg cccagtacgt cggcgtactc tacgtcgaac
      601 agcccgtcgc cgttcaggtc gtaggattga cgacgcaacg cccgcccgat tacctgctcg
      661 cacaggagct gggtaccgaa agcgcgcacg cccaggacgt gggtcaccgt gttcgcgtcc
      721 cagccttccg ttagcatcga gacggacacg acgcaacgta tggactcacc gaggtgtcct
      781 ggcttgccta cggtgttcat gacctcgcgg agtatatcct ggtcgctgat ctcgtcggcc
      841 ttcttgcggt ctccggtacg ctcgatgatc tcgcgacgaa aaagggatag ttcttccgac
      901 gccgccgcga ggaagctatc gtccagcttt ccgcctgact ccagctgttg cgagtcgata
      961 agcagggtcc tcggcctcga tagcgcggag ccgtcctccg cgaagttccg gaagagcggc
     1021 aagcgtccat tctcgaatcg ctcgctcccg tcgccgttgg tccgcctgaa gccggagatg
     1081 tagtcgaaga cgagctttga cgacgcggta ttgttacaga cgattatgaa acagggcggc
     1141 gagtcgagcc cggcctcctg ccatagattg aaggtcttct cgtaatgtcc gtagagagcg
     1201 tccagcgccg tctgcagctc gaccgggata gacaggggat cgagcgatcc tgacttaccc
     1261 cgtcccttct tgggcatctt cggaccgacc aggtcccata gcttgcggta cttgggcatg
     1321 tcaccgccgg ctatattgtc cgccactggc acgcgcggta gcttgacgat gccgcactct
     1381 atggcgtcca tgagggagaa gtcgctcgcc gtccacggga agagagttcc ttcggcatat
     1441 cctgatcccc taaggaagaa cggagtagct gaaaggtcta ccacgcgcgt tacgcctagc
     1501 ttgtcctgga cggcctcgat gcccgagatc cagacgcgag cggcctcggc gttctccttc
     1561 gcctcgtcct tctcgtcgga gcctatcgtt tcttcttcgt cgtcgatgcc ctttggtttt
     1621 tccctatagc aatggtgcgc ctcgtcgttg atgacgagga tgtccttaag ccccatcagc
     1681 tctggcatga cgcgttggag catctggcct tcggtctcca aagtctgtag gtccggtccg
     1741 cggcccttga ggagggaacg cccgcccttc gaaagcgtga cgcgttcccg gagcatgaag
     1801 gcatggtagt tcgtgatgac tatcttggcc ttggaaagct cgcctagaag ctcggtcggc
     1861 acgagctcgc ggctggcgta gtagctgttc gggtcgttgg gcctgaggac ctgcaggcgg
     1921 tcccgtatcg tgataccggg cgagatgatc aggaagccct tggtaaagcg cttgctctgc
     1981 gggtgccgta cggcgttaat cgtctgccag gctatgatca tggccatcac ggtggtcttg
     2041 cccgtgcccg tggcaagctt gagcgccagt cggaagagcc caggattggc ggcttcgctc
     2101 gcggcaccga aatgctcgat gaaggccgat cccgtctttc cagacgtcgg agccacctcg
     2161 gtcagccaga tgagcgtctc gacgg
//
LOCUS       DAOPHV010000481         2182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_71078_length_2182_cov_3.634697, whole genome shotgun sequence.
ACCESSION   DAOPHV010000481 DAOPHV010000000
VERSION     DAOPHV010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71078_length_2182_cov_3.634697"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..951
                     /locus_tag="PLQ29_11110"
     CDS             277..951
                     /locus_tag="PLQ29_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPG87236.1"
                     /translation="MAARKGRPREPGSAILPAGQTTQLVVGASPESDATILTLAENLY
                     LAYDVRRVYYSAFVPAGLDPRVPALPGPPLLREHRLYQADWLFRFYGFRADEILGPDR
                     PNLDADLDPKCAWALRHLDRFPIDVNHADYLELLRVPGIGPRSASRIVKARAGARLMP
                     ESLKALGVVMKRAAFFITCSGAVPSLPGSSRRPVPDASYARAALTDGARRALAPLQME
                     FDWGEP"
     gene            948..1763
                     /locus_tag="PLQ29_11115"
     CDS             948..1763
                     /locus_tag="PLQ29_11115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HPG87237.1"
                     /translation="MTETVDYRAEPATLAHDGSWPGFLCACAEALNASEPVPRVVDAD
                     APVGLFESRIAVARDDGRAAALWDRLTIKAGAPAMRIVLEAFLSERPDARADTASVLR
                     RLRREGAAALRDLADPIVLSVDKAATRARREAERTTGIARFSELADGSWYAPLEPGCD
                     VLPLVADHFAARFRPMRFALHDLRRGRAVLHEPGRPWLLTDGFSLNQVEPDEDAGPGA
                     IDSAMLASDELRIRDLWRRYFAATAIEGRLNPGLQAAHIPKKYWKLLTEMRRG"
     gene            complement(1923..>2182)
                     /locus_tag="PLQ29_11120"
     CDS             complement(1923..>2182)
                     /locus_tag="PLQ29_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG87238.1"
                     /translation="VASRIVGLDSLVHQVDLAMAEQKAGTSKVLEALQSMNEITHQVK
                     SGSAEMSAGNGIVLSEISRLQDSTRQIIASVEDMSGGVRRL"
BASE COUNT          321 a          787 c          713 g          361 t
ORIGIN      
        1 cggttccatc cgttacaatg cccgcatcat gcagcagtgt cgcgcggata atgccgaaag
       61 cctcaccggc tcggtcgagc gggtgacgtt tcacagcgcc gagacgggct tctgcgtgct
      121 gcgcgtggcg gtccgggggt ttcgcgattt ggtgaccgtc gtcggcacgc tgccggagcc
      181 ccgggccggc tcgccctgcc cgcgccgacg gtatcggcgg ccgagccggc accgatctac
      241 gccgcccgcc tcgaaccgcc ggtcgggcgt tccgtcatgg ccgccaggaa aggccgaccg
      301 cgagaacccg gctccgcgat cctgccagcc ggccagacga ctcagctcgt cgtcggcgcc
      361 agccccgagt cggacgccac gatcctgacc ctggccgaga acctctacct ggcctacgac
      421 gtgaggcgcg tctactactc ggcgttcgtg cccgcgggcc tcgacccgcg cgtgcccgcc
      481 ctacccggcc caccgctctt acgggagcac cggctgtatc aggcggactg gctcttcagg
      541 ttctacggct tccgtgccga cgagatactc ggtcccgatc ggcctaacct cgacgcggat
      601 ctcgacccga agtgcgcctg ggccctccgc cacctcgacc gtttccctat cgacgtcaac
      661 cacgccgact acctcgagct actgcgcgta ccgggcatcg gcccgcgctc ggcctccagg
      721 atagtcaagg cgcgggccgg cgcgaggctc atgccagagt cgctcaaggc cctgggcgtc
      781 gtgatgaaac gggccgcgtt ctttatcacc tgctcgggtg cggtcccaag cctacccggt
      841 tcctccaggc ggccggtccc cgacgcgtcg tacgccaggg ccgcgctgac cgacggcgcg
      901 aggcgagcgc tggcaccgct ccagatggaa ttcgactggg gcgagccttg accgagacgg
      961 tggactatcg ggccgagccg gcgacgctgg cccacgacgg tagctggccg ggcttcctct
     1021 gcgcgtgcgc cgaggcgctc aacgcgtccg agccggttcc gcgcgtcgtc gatgcggacg
     1081 cgccggtggg gctgttcgaa tcgcgcatcg ccgtggcgag ggacgatggg cgagccgcgg
     1141 ccctgtggga caggctgacg atcaaggcgg gcgccccggc gatgcgtatc gtgctcgagg
     1201 cctttctgtc ggagcggccc gacgcccggg ccgacacggc ctccgtcctg cggcgccttc
     1261 gccgcgaggg cgccgctgcc ctgcgcgacc tggccgaccc catcgtcctg tcggtcgaca
     1321 aggccgctac cagggcgcgc agggaggccg aacgaacgac gggcatcgcg cgcttctcgg
     1381 agctggccga cggctcctgg tacgcgccgc tcgagcctgg atgcgacgtc ctacccctcg
     1441 tcgcggatca tttcgcggcc cgcttcaggc cgatgcgatt cgctctgcac gacctgcgca
     1501 ggggccgagc cgtgctacac gagccgggcc gcccctggtt gctgacggac gggttctctc
     1561 taaatcaggt agagcccgac gaagacgccg ggcctggcgc gatcgattcg gccatgctgg
     1621 cttcggatga attgcgcatc cgcgacctct ggcggcgata cttcgcggcc acggccatcg
     1681 aagggcggct caaccctggc cttcaggccg cccatatccc gaagaagtac tggaagctcc
     1741 ttaccgagat gcgccgggga tagcggctac ggacactgac ttggggccga tcttgacgct
     1801 caggaatgcc gggtcgctcc aagctcggag ccctccgtcc tgaaaccccg cagggcttcg
     1861 tccatgccgc cgatagtcgc ctcggtatca tgggctatcg tcgatatcat cgcggcgcgc
     1921 tcctagagac gcctgacccc accggacata tcctcgacgc tggctatgat ctgcctcgtc
     1981 gagtcttgca gcctcgatat ctccgacaag acgatgccgt tcccggcgct catctcggcc
     2041 gagcccgact tgacctgatg cgttatctcg ttcatggact gcagggcctc gagcaccttg
     2101 gacgtgccgg ccttctgctc ggccatggct aggtcaacct ggtgaaccag ggaatcgagg
     2161 ccgacgatac gcgacgccac cg
//
LOCUS       DAOPHV010000482         2167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_71782_length_2167_cov_4.054451, whole genome shotgun sequence.
ACCESSION   DAOPHV010000482 DAOPHV010000000
VERSION     DAOPHV010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71782_length_2167_cov_4.054451"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PLQ29_11125"
     CDS             <1..477
                     /locus_tag="PLQ29_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87239.1"
                     /translation="DLFLAVIDMGSDALERELADIDPSAGFFAGLEAMLRKARHAADR
                     YPELNQIYLDSTTQGLSTLAARLTNRLEAVTARLYRRLFAAGQASGELRPDIDPGYAA
                     FCLDNLIVMLQFSYSSDYYRDRMRIMVGVGDDEEVDEDKLVSCVLAMARGAFGAFR"
     gene            474..1439
                     /gene="truA"
                     /locus_tag="PLQ29_11130"
     CDS             474..1439
                     /gene="truA"
                     /locus_tag="PLQ29_11130"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPG87240.1"
                     /translation="MKAQPGQPGRERNYLLTLAYDGSGFSGWQRLGGGARTVQKTLED
                     ALSSMLGERINVIGAGRTDAGVHAEGQAANFRTVSGLGADELRSALDGALPPDLCCVS
                     VREAVPEFHARYRAVSKTYAYRFHDGPIRDPFEARWSLHVGRRLDVSAMREACAALVG
                     ERDYSALSNAKEDARGFVRNLFEARVERRGDLVEVGFRADGFLYNQARVMAACAMEAG
                     LGRMPPGRLAELVASKDRSRAPGALGAYGLRLLRVEYRADDFSGPGSGRPEPRRPDRD
                     EGPPPGQARVRSDGLSSGRPSGEPGRGDRRPRGCDGRSPRSPEPR"
     gene            complement(1709..2155)
                     /locus_tag="PLQ29_11135"
                     /pseudo
     CDS             complement(1709..2155)
                     /locus_tag="PLQ29_11135"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
BASE COUNT          301 a          767 c          767 g          332 t
ORIGIN      
        1 gacctgttcc tcgcggtcat agacatgggc tcggacgcgc tcgagcggga gctggccgat
       61 atagacccat cggccggctt cttcgccggc ctcgaggcca tgctgcgcaa ggccaggcac
      121 gcggccgacc gttatcccga gctcaaccag atctacctgg actcgaccac gcaggggcta
      181 tcgacgctgg ccgccaggct gacgaaccgg ctcgaggcgg ttaccgcgag gttgtaccgc
      241 cggctgttcg ccgccggcca ggcttccggc gagctacgtc ccgatataga ccccgggtat
      301 gccgccttct gtctggacaa cctgatcgtg atgctgcagt tctcatactc gagcgactac
      361 taccgcgacc gcatgcgcat catggtcggc gtcggcgacg acgaggaggt cgacgaggac
      421 aagctcgtgt cctgcgtcct cgcgatggcg cgcggcgcgt tcggcgcatt ccgatgaagg
      481 cccagccggg ccagccgggc cgcgagcgca actacctatt gacgctagcc tacgacggct
      541 cgggcttctc cggttggcag cggctcggcg gaggcgcccg taccgtgcag aagaccctcg
      601 aggacgccct ttcgtcgatg ctgggcgagc ggatcaacgt gataggcgcc ggacggaccg
      661 acgccggcgt gcacgccgag ggccaggcgg ccaacttcag gaccgtatcg ggcctcggtg
      721 cggacgaact ccgctccgcc ctggacggcg cgctgcctcc ggatctgtgc tgcgtctccg
      781 tccgcgaggc cgtccccgaa ttccacgccc gctaccgggc ggtgtccaag acctacgcct
      841 accgtttcca cgacggcccc atccgcgacc ccttcgaggc gcgttggtcg ctgcacgtcg
      901 gccgccggct cgacgtgagc gcgatgcgcg aggcctgcgc ggctctggtc ggcgagcgcg
      961 attatagcgc cctgtcgaac gccaaggagg acgcgcgcgg cttcgtccgg aacctgttcg
     1021 aggcgcgggt cgagcgccgc ggcgatctgg tcgaggtcgg cttccgcgcc gacggcttcc
     1081 tctataacca agcgcgcgtg atggccgctt gcgccatgga ggccggtctg gggcgtatgc
     1141 cgccgggcag gctcgccgaa ctcgtcgcgt cgaaggatag gagccgagcc cctggcgccc
     1201 tcggggccta cggcttgcgc ctgctacgcg tcgaatatcg cgccgatgac ttctcgggcc
     1261 cgggctccgg tcgcccggag cctcgccgcc cggatcgcga cgagggcccg ccgcctggcc
     1321 aggcgcgggt ccgatccgac ggactatcct cgggccggcc gtcgggcgag cccggccggg
     1381 gagatcgacg gccgcgaggc tgcgacggac gctcgccgcg atcgcctgaa ccgcgctgat
     1441 cgctcgctgg cgcccggcgc ttcggcgccg ggcttgatcg tcgcccggaa acggcgcccg
     1501 aaggggagcc cgaaggggag cccgaagggg agccggccat cgcggccgcg aaggacgcag
     1561 gctcgggaca gtctatccgt atcgtcgctc cgtcggacgg acgcttgagc tcgatcagcg
     1621 tcgcgtgcag ggctagcctg cctatcgggt cgcggtggga gccgtagcgc tcgtcgccgg
     1681 cgacggggca gcctatcgcc gccagctggg cgcggatctg gtgcctgcgc cccgtctcca
     1741 gggacagctc gacgaggcag aggccgtcgc cccgggcccg tacccggtaa cgcgtaacgg
     1801 cgcgcagcgc cccgcgcgta cccggcggga cctgcctgac ccgatacgga tcggcgtccg
     1861 acagccagct atcgagtaca ccgtcgtcgt tcggcggcgc gccctcgacg agtgccgtgt
     1921 agcgacgctc gtcgaccgac tcgttccatg ccgacatcag cgcggccttg gcgcgggcgt
     1981 tcttggcgaa cacgatgacg cccgaggtgt cgcggtcgag gcggtgcacc acggccgccc
     2041 gacccttggg gttgcgcctg cgtatgtggg ccgtgacgac gtcgtagagg ctcttggacc
     2101 gggagccctc gggcgctatg gtcgctagcc cgcagggctt atcgacggct accaggtcgt
     2161 cgtcctc
//
LOCUS       DAOPHV010000483         2163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_71969_length_2163_cov_3.555503, whole genome shotgun sequence.
ACCESSION   DAOPHV010000483 DAOPHV010000000
VERSION     DAOPHV010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71969_length_2163_cov_3.555503"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /locus_tag="PLQ29_11140"
     CDS             <1..99
                     /locus_tag="PLQ29_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HPG87241.1"
                     /translation="VLEVTESRISQLHTKAILRLRARLTHYRKGIV"
     gene            101..2089
                     /locus_tag="PLQ29_11145"
     CDS             101..2089
                     /locus_tag="PLQ29_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPG87242.1"
                     /translation="MEARIGFDAVRELMRRYLDEDGDKRAIVAEGATLEEALRSASVQ
                     LDCPVARLEYDILERGAQGFVGLGRKKWKLSVYEAAVKQKVEESSIESFDGGLDLPIE
                     LKPIQQDVDGQVYVRLATEGALLKVAEPQGRGRRATDKQAVEKLHARAIHDFDEALVR
                     ETVKRAAGEWVVVGDFIANPAADALLTVDIGSQEMEARVIITPPQPGGCDLSKDVILA
                     YLRNNKVVFGVIEEALQGLEDAPRYKEPVLVAQGQRAVNGDDARIQFLFETDRTKLNL
                     EEKNGKVDYKELHLVQNVVEGQALAKKVQAKQGVPGRTVTGKLLPAKNGKDLPLPLGK
                     NVHESDDGLTVIADVNGEATYINSKINVETVYTINGDVDLKTGNQFFLGTIVVVGNVE
                     DGFSVKATGNIEVRGNVGKAEISAEGDVIVHQGITGKGAGVITAGKNVWAKFIENAQL
                     YAGENVIVQTSIINSEITAEKRILCSGQKHAAIIGGRYRACEEINAKSIGSPSGGAET
                     ILEVGSDPKSKLKMDELDGKMKLLQRQIDELDKNIYTLNETKRQRKTLSDDKQAVLDE
                     LTHRRDELKQESSSLKDEYDALQAYLNNLKVRGKVSVSGRIYPGSEIVIRDIREKIKN
                     EYKGLTFYLENMMIKTTRYEEFDDEILRKGPPDAHKAD"
BASE COUNT          482 a          697 c          683 g          301 t
ORIGIN      
        1 gtgctcgagg taaccgaatc gaggatcagc caactccata cgaaggccat cctgcgccta
       61 cgcgcgcgac tgacgcacta ccggaagggc atcgtttagt atggaggcgc gcataggctt
      121 cgacgcggtc cgcgagctga tgcgccgtta cctggacgag gacggcgaca agcgcgcgat
      181 agtagccgag ggcgccacgc tcgaggaagc gctccggagc gcttccgtcc agctcgactg
      241 cccggtggcc aggctcgagt acgacattct cgagcgtggg gcgcagggct tcgtcggcct
      301 gggccgaaag aaatggaagc tgagcgtcta cgaggccgcg gtcaagcaga aggtcgagga
      361 gtcgtcgatc gagtccttcg acggcggcct cgacctgccg atcgagctca agcccatcca
      421 gcaggacgtc gacggccagg tctacgtgcg gctcgccacc gaaggcgccc tgctcaaggt
      481 agccgagccc cagggcaggg gccgccgcgc caccgacaag caagccgtcg agaagctcca
      541 cgcgcgcgcg atccacgact tcgacgaggc gctcgtgcgc gagacggtca agcgggcggc
      601 cggggaatgg gtggtcgtcg gcgacttcat cgccaatccc gccgccgacg cgctcctgac
      661 cgtcgacatc ggctcccagg agatggaggc ccgcgtcata atcacgccgc cgcagcccgg
      721 cggatgcgac ctgtccaagg acgttatact cgcctacctg cgcaacaaca aggtcgtctt
      781 cggcgtgatc gaggaggcgc tccagggcct cgaggatgct cctcgctaca aggagcctgt
      841 cctcgttgcc cagggacagc gcgccgtcaa cggcgacgat gcccgcatcc aattcctgtt
      901 cgagaccgac cgcaccaagc tgaacctcga ggagaagaac ggcaaggtgg actacaagga
      961 gttgcacctc gtccagaacg tggtcgaggg gcaggccctg gccaagaagg tccaggccaa
     1021 gcagggcgtg ccgggccgta ccgtgaccgg caagctctta ccggccaaaa acggcaagga
     1081 cctgccgctt ccgctcggca agaacgtcca cgagagcgac gacggcctga ccgtcatagc
     1141 ggatgtcaac ggcgaggcca cctacatcaa cagcaagatc aacgtcgaga ccgtctatac
     1201 catcaacggg gacgtcgacc tcaagaccgg aaaccagttc ttcctcggca ccatagtcgt
     1261 cgtcggcaac gtcgaggacg gcttctccgt caaggccacc ggcaatatcg aagtgcgcgg
     1321 caacgtcggc aaggccgaga tcagcgccga gggcgacgtc atcgtccacc agggcatcac
     1381 cgggaagggc gcgggcgtga tcacggccgg caagaacgtc tgggccaagt tcatcgagaa
     1441 cgcgcagctc tacgccggcg agaacgtcat cgtccagacc agcatcatca actcggagat
     1501 caccgccgag aagcgcatcc tgtgctccgg ccagaagcac gccgccatca tcggaggccg
     1561 ctaccgcgcc tgcgaggaaa tcaacgccaa gtcgatcggc tcgccgtcgg gcggtgccga
     1621 gacgatactc gaggtcggca gcgacccgaa gagcaagctc aagatggacg agcttgacgg
     1681 caagatgaag ctgctccagc gccagatcga cgagctggac aagaacatct ataccctgaa
     1741 cgagaccaaa cgccagcgca agacgctttc ggacgataag caggctgtcc tggacgagct
     1801 gacccaccgc cgtgacgagc tcaagcagga gtcctcgtcg ctcaaggacg agtacgacgc
     1861 cctgcaggcc tacctgaaca acctgaaggt tcgcggcaag gtcagcgtct cgggcaggat
     1921 ctaccccggc tcggagatcg tgatccgcga catccgcgag aagatcaaga acgagtacaa
     1981 gggcctgacc ttctatctcg agaacatgat gatcaagacc acgcgctacg aggagttcga
     2041 cgacgagatc ctccggaaag gaccgccgga tgcccataaa gccgattgac ctgcagaccc
     2101 tgttcttgca gttgtcccag gtcggcaagg agcaggcggc ggccaaggac ggggcactgc
     2161 tcc
//
LOCUS       DAOPHV010000484         2161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_72056_length_2161_cov_4.443970, whole genome shotgun sequence.
ACCESSION   DAOPHV010000484 DAOPHV010000000
VERSION     DAOPHV010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72056_length_2161_cov_4.443970"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PLQ29_11150"
     CDS             complement(<1..807)
                     /locus_tag="PLQ29_11150"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG87243.1"
                     /translation="MRKLPAIIRYEFKLMAANKAFVILTILGPFLILAVTVLPGLLSM
                     NPKALSGGEPIAIAASSGSGVVGESAALASAFETALAAQGRGVEAVSLDEASIADAKK
                     RVGSGKLAALVVLPVGWEDSGATLFTKTGTDAMLYGLVERVVDAFARESRIARSGIDP
                     DVARALLNGVTLSIVKLSAGDREEAGGEGAFVQTLFTAMSFVMLVYMTTLLYGQMIGR
                     SVVMEKTSKTVEIMLSSVSPRELMFGKILGLGLAGLLQYGAECLPENPKLF"
     gene            complement(800..1729)
                     /locus_tag="PLQ29_11155"
     CDS             complement(800..1729)
                     /locus_tag="PLQ29_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG87244.1"
                     /translation="MLSVEKVVKTYETVRAVDGLSFEARPGEIFGLIGPNGAGKSTTI
                     RMIMNVIAPDDGTIRFDGKGFSEAMKERIGYLPEERGLNRKVKVIDTLVYLGVLKGLE
                     AADARRSAESWLERFELSDWKGEKVEALSKGMAQKVQFIGSVLHDPDLVLFDEPFSGL
                     DPVSQDTLLEAVLELKAKGKTVLFSTHVMEHAERLCDRILLVRKGRELVSGSLAELKS
                     GYGTNSVQIEFDGDGSFIANLPCVVDVRTMPRWIEASLAEGHGVDELFSALSGRVSVR
                     RFQLMEPSLHALFVSLVESSDDAGTDDKEAAHA"
BASE COUNT          412 a          688 c          641 g          420 t
ORIGIN      
        1 gaagagtttc gggttctctg gcagacattc cgcgccgtac tggaggagtc cggctagtcc
       61 gagcccgagt atcttgccga acatgagctc gcggggcgat acgctcgaca gcatgatctc
      121 gacggtcttg gaagtcttct ccatgaccac ggatcggcct atcatctggc cgtagagcaa
      181 cgtcgtcatg tagacgagca taacgaagct catcgcggtg aatagcgtct ggacgaaagc
      241 gccctcccct ccggcttctt ctcgatcgcc agccgacagc ttgacgatgg acagcgtgac
      301 gccattcagc aaggccctgg ctacgtccgg gtcgatgcca ctcctggcga tgcgcgattc
      361 cctggcgaag gcgtcgacga cgcgttccac caggccgtag agcatcgcgt cggtgcctgt
      421 tttggtaaag agtgtggcgc ccgagtcttc ccatccgacc ggcagcacga ccagagcggc
      481 cagtttcccg gaaccgacac gcttcttggc gtcggctatg gacgcctcgt cgagggatac
      541 ggcctcgacg ccgcggccct gagccgcgag ggccgtctcg aacgcggagg ctagcgccgc
      601 ggactcgccc accacgcccg aaccggaaga agccgctatc gcgatgggct cgccgccgga
      661 gagggccttc gggttcatcg atagaagtcc gggcagcacg gttacggcca gtatcaggaa
      721 cggcccgagg atggtcagta tgacgaaggc cttgttcgcg gccatcaact tgaactcgta
      781 gcggatgatc gccggtagct tacgcatgag cggcctcctt gtcgtctgtc ccggcgtcgt
      841 ccgacgactc aaccaagctg acgaacaggg cgtgcagcga cggttccatc agctggaagc
      901 gtctcacgct caccctgccc gatagggcgg agaatagctc gtcgacgccg tggccctcgg
      961 ccaggctagc ctcgatccac cgcggcatcg tcctgacgtc tacgacgcag ggcaggttcg
     1021 cgatgaacga gccgtcgcca tcgaactcga tctgaaccga gttggttccg tagccggact
     1081 tgagctcggc cagcgagccg gagacgagct ccctgccctt gcgtaccagg agaatgcggt
     1141 cgcatagacg ctcggcatgc tccatcacgt gagtggagaa cagtaccgtc ttgcccttgg
     1201 ctttcagctc gagtaccgcc tcgaggaggg tatcctgcga caccgggtcg aggccggaga
     1261 acggctcgtc gaacaacacc aggtcggggt cgtgcaggac cgagccgatg aactggacct
     1321 tctgagccat gcccttcgag agggcctcga ccttttcgcc cttccagtcg gacagctcga
     1381 agcgctcgag ccacgactcc gcgctccgcc tggcgtcggc cgcttcaagg cccttgagta
     1441 ccccgaggta gacgagcgtg tcgatcacct ttaccttgcg gttgagccca cgctcctcgg
     1501 gcaggtagcc gatgcgctcc ttcatggcct ccgagaagcc cttgccatcg aagcgtatcg
     1561 tcccgtcgtc cggggcgatc acgttcatga tcatgcgtat cgtcgtcgat ttgccagctc
     1621 cgttcgggcc tatcagtccg aagatttctc ccggccgagc ctcgaacgac agcccgtcga
     1681 cggcgcgcac cgtctcgtag gtctttacga ccttctctac gctgagcacg gttcactcct
     1741 tgttcgcccg cgcggcgatc cgccgcgggc gcatgagata accgtagtat aactgcaaat
     1801 tcctcgatag catactccgt tctcgcttgc cgcagcccgc tccaggtcct atactcgctt
     1861 ccgtgctgac aatgcgtaca ggcgcgaagt cgcggcccgc actgcttatg gtgtgaggaa
     1921 attgaagtgt tacaaacctt gtgtatccaa ggagcgtcca gctgcttgcg actacgaggt
     1981 attggttcta cagtctcgga tggcgatctg cgcggctaac tcgttcacaa acgctatgtt
     2041 acgcacaacg gatacataat tttcgaacca ctacccaagg agaccatcga catgacgtac
     2101 aacgaggtgc gggccaaggc taagcagacc atcggaccgt attgcaaggt atgcccggag
     2161 t
//
LOCUS       DAOPHV010000485         2158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_72200_length_2158_cov_4.068949, whole genome shotgun sequence.
ACCESSION   DAOPHV010000485 DAOPHV010000000
VERSION     DAOPHV010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72200_length_2158_cov_4.068949"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="PLQ29_11160"
     CDS             <1..1062
                     /locus_tag="PLQ29_11160"
                     /inference="COORDINATES: protein motif:HMM:NF023591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87245.1"
                     /translation="DAYTLFDQVASFSDGRITAAKIRDKLGLVGSERMGALMSLCVSG
                     DRKGALETLDAILDSGVSVEQFASDAVSYIRSLLLVASGIERESLLGAPRSSFPAALT
                     EALDAPRLERALSGLFDLYRDMRYSVNPRWELELAVSRLCAIRDYVSSAELSRTIRGM
                     KAAIVEGRAPGAAAVGAALREASAVSPRIEAAAAERPDPAAAGAEPPAENPGSATPGL
                     DVDGIDTVALRASLLAAVGKNRVVLSTCLERSGDWVVDGDKLVIHCLNGYDAGAVLAD
                     AGLLAKKALDITGRPLRIEPRAAEPAPAPTPPARSGDDEDSDDGDDGPVDPVGIVEKV
                     FRGRRVSAPASNERSESWT"
     gene            1053..1367
                     /locus_tag="PLQ29_11165"
     CDS             1053..1367
                     /locus_tag="PLQ29_11165"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPG87246.1"
                     /translation="MDMSDIMKMLKDPQALQRQALEAQERMAAISAVGSAGGGMVRVT
                     LSGTMELLNVEIAPEAIDPSDPAMLQDLIKAAHNDASYRVKEALQADVARNLGSMPGL
                     GR"
     gene            1364..1951
                     /gene="recR"
                     /locus_tag="PLQ29_11170"
     CDS             1364..1951
                     /gene="recR"
                     /locus_tag="PLQ29_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPG87247.1"
                     /translation="MKALEDLVGILARLPGIGRKSAARIAFHILRSDDQYVALLAERI
                     GSLKERVRFCSVCGSYAEDDPCEVCSSPMRDRSLICVVEQAQDVLTIEASREYRGVYH
                     VLGALISPLDGIGPDALRLDTLVARVRDQGVAEVVIATNPTVEGDTTALYIKKVLEGC
                     GARVTRLATGIPVGGDLEYADKLTLARSFRGRVAL"
     gene            complement(1948..>2158)
                     /locus_tag="PLQ29_11175"
     CDS             complement(1948..>2158)
                     /locus_tag="PLQ29_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87248.1"
                     /translation="LGKRRTKPGISREALNEAENLVYEFMDRNYANAYMDWESSEHRK
                     RVAALGFSLDGLDEVVEACYRRLGS"
BASE COUNT          354 a          701 c          740 g          363 t
ORIGIN      
        1 gatgcatata cgctgttcga ccaggtagcc tcgttctccg acgggcgtat taccgccgcc
       61 aagatccgcg ataagctcgg cctcgtcggt agcgagcgca tgggcgccct gatgagccta
      121 tgcgtgtccg gggacaggaa gggcgccctc gagaccctcg acgccatcct cgactcgggc
      181 gtgtcggtgg agcagttcgc ttccgacgcc gtctcctaca tccgatcctt gctgctcgtc
      241 gcgagtggga tcgagcggga gagcctcctg ggagccccca gatcgtcgtt cccggccgcc
      301 ttgaccgagg cgctcgacgc gccgcgcctg gagcgagcgc tgtccggcct gttcgacctg
      361 taccgggata tgcgctatag cgtcaacccg cgctgggagc tcgagctggc cgtctcgagg
      421 ctctgcgcca ttcgcgacta cgtttcgtcg gccgagctgt ccaggaccat tcgcggcatg
      481 aaggccgcca tcgtagaggg ccgggcgccc ggcgcggcgg ccgtaggggc cgcgcttcgc
      541 gaagcgtcgg ccgtttcgcc ccgcatcgag gccgcggcgg cggagcgacc cgatccagcc
      601 gccgccggcg ccgagcctcc ggcggagaat ccggggtcgg ccacgccggg tttggacgtc
      661 gacggcatcg ataccgtggc gctcagggct tcgctactgg cggccgtcgg caagaacagg
      721 gtggtcctgt ctacctgcct ggagcgttcc ggggactggg tagtggacgg agacaagctg
      781 gtcatccatt gcctgaacgg ttacgacgcc ggcgccgtgc ttgccgacgc cggcttgttg
      841 gccaaaaagg cgctggacat taccggtcgg cccctgcgta tcgagcctcg agcggcagag
      901 cccgccccgg cgccgacgcc tccagcgagg tcgggcgacg acgaagactc ggacgacgga
      961 gacgacgggc ctgtcgatcc ggtaggcatc gtcgagaagg ttttccgcgg ccggcgcgtc
     1021 tcggcgccgg cgagcaacga acggagcgaa tcatggacat gagcgatatc atgaagatgc
     1081 tgaaggatcc gcaggccctg cagcgccagg ccttggaggc ccaggaacgc atggccgcca
     1141 tcagcgcagt cggttcggct gggggcggga tggtccgcgt cacgctgagc gggaccatgg
     1201 aactcctgaa cgtcgagata gcgcccgagg cgatagaccc atccgacccg gctatgctcc
     1261 aggacctgat caaggcagcc cacaacgacg cgtcctacag agtcaaggaa gcgctacagg
     1321 ccgacgtcgc ccgtaacctc ggctcgatgc cgggcctggg ccggtgaagg cgctcgagga
     1381 cctcgtcggt atcctggcca ggttgccggg cataggtcgg aagagcgccg ccaggatcgc
     1441 ctttcatata ctcaggtctg acgaccagta cgttgcgctc ctggcggaac gtatcggttc
     1501 gcttaaggaa cgagtgcgct tctgctccgt ctgcggttcg tacgccgagg acgacccctg
     1561 tgaggtctgc tcctcgccga tgcgcgaccg aagcctgatc tgcgtggtgg agcaggcgca
     1621 ggacgtgctg accatcgagg cctcgcgcga gtaccggggc gtctaccatg tgctcggcgc
     1681 cctgatctcg ccgctggacg gcataggccc cgacgcgctc aggctcgata cgctggtcgc
     1741 cagggtgcgc gaccagggtg ttgccgaggt ggtcatagcg accaatccga ccgtggaggg
     1801 cgatacgacg gccctctaca taaagaaggt gctcgagggc tgcggggcga gggtgacgcg
     1861 cctggcgacc ggaatcccgg tcggcggcga cttggaatat gccgacaagc tgacattggc
     1921 caggtcgttc cgcggccgcg tcgcgctcta ggagcccagc ctccggtaac aggcctcgac
     1981 gacctcgtcg aggccgtcga gcgagaagcc gagcgctgcg acgcgcttcc gatgctctga
     2041 cgactcccag tccatgtacg cgttggcgta gttcctgtcc atgaactcat agacgaggtt
     2101 ctcggcctcg ttcaacgctt cacgcgatat ccccggcttg gttcgacgtt taccgagt
//
LOCUS       DAOPHV010000486         2158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_72210_length_2158_cov_3.283880, whole genome shotgun sequence.
ACCESSION   DAOPHV010000486 DAOPHV010000000
VERSION     DAOPHV010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72210_length_2158_cov_3.283880"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLQ29_11180"
     CDS             <1..285
                     /locus_tag="PLQ29_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPG87249.1"
                     /translation="GNDDAIRSISLFTQIIANAVVEAESETGLKIIETLQDEDGAEEE
                     AKAAVEDEEEEVEVDPATYSAEPAASAASEVAAEGAAEEPIVEEDKYYEK"
     gene            296..1150
                     /gene="tsf"
                     /locus_tag="PLQ29_11185"
     CDS             296..1150
                     /gene="tsf"
                     /locus_tag="PLQ29_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPG87250.1"
                     /translation="MDIKASDIKNLREKTGAGMMDCKKALVEAEGDFNLAERKLKEWG
                     LAGVAKRSGRATNEGRVFIAEGADAIAVVEIACETDFVCRNDDFIAGGDRMAKLALAK
                     GYASANDELEAIVKDMASVIKENNTLKSVTLVKAGAAEYVHSYVHGEGKLAVVVKMRA
                     DKAEAFKNASVTGFVHDLALHVAAFRPMFVDQGRIGADWIKEQQEIFQKQVEGDEKMV
                     GKPAKVIEGILAGKLKKLMSEICLVDQGFVKDEKQTVAAVMASIAKAAGTQLSIVDYA
                     YIKVGDNQ"
     gene            1233..1775
                     /gene="frr"
                     /locus_tag="PLQ29_11190"
     CDS             1233..1775
                     /gene="frr"
                     /locus_tag="PLQ29_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPG87251.1"
                     /translation="MEDIKNACEERMKKTVASLKDDFAAIRTGRASPSILDKLRVDYY
                     GQKSPISQVATVSVPEARLIVVQPWDRALIVEIEKAIQKSELGLNPSNDGKVIRIAIP
                     PLTEQRRKELVKSAKSSAETSRVGIRNIRRDGLEDIKKAGAPEDEVKKAEDELQKLTD
                     SFIAQINKVLEDKEKEILEV"
     gene            1778..>2158
                     /gene="uppS"
                     /locus_tag="PLQ29_11195"
     CDS             1778..>2158
                     /gene="uppS"
                     /locus_tag="PLQ29_11195"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HPG87252.1"
                     /translation="MQPTLARSVPAHVAVFMDGNGRWAKARGLPRTDGHREGLKAAKR
                     VVKAAKDAGVRYLTLYTFSTENWKRAQEEVKFLMGLVSTYLCAELDFYRENGVRVVHS
                     GDDEGLPKDVREALERVSDDTAGFD"
BASE COUNT          491 a          650 c          697 g          320 t
ORIGIN      
        1 ggaaacgacg acgccatccg ctcgatctcc ctgttcacgc agatcatcgc caatgcggtc
       61 gtcgaggccg agagcgagac cggcctcaag atcatcgaga ccctgcagga cgaggatggc
      121 gccgaggaag aggccaaggc cgcggtagag gacgaagagg aagaagtcga ggtcgacccg
      181 gctacctaca gcgccgaacc ggccgcctcg gccgcttccg aggtagccgc cgaaggcgct
      241 gccgaggagc ccatcgtcga ggaagacaag tactacgaga aataaggagc ataccgtgga
      301 tataaaggct agcgacataa agaacctgcg cgagaaaacc ggcgcgggta tgatggactg
      361 caagaaagct ctcgtagaag ccgagggcga cttcaatctg gccgagcgga agctcaagga
      421 gtggggcctc gcgggcgtcg ccaagcgctc cggccgcgcc accaacgagg gccgcgtctt
      481 tatcgccgag ggcgccgacg ccatcgcggt cgtcgagatc gcctgcgaga ccgacttcgt
      541 gtgccgcaac gatgacttta tcgcaggtgg cgatcgcatg gccaagcttg ccctcgctaa
      601 gggctatgcg tcggccaacg acgagctcga ggccatcgtc aaggatatgg cgagcgtcat
      661 caaggagaac aacacgctca agagcgtcac gctggtcaag gccggcgccg ccgagtacgt
      721 ccactcctac gtccacggcg agggcaagct cgccgtcgtc gtcaagatga gggctgacaa
      781 ggccgaggcg ttcaagaacg cttcggtaac cgggtttgtc catgacctgg ccctgcacgt
      841 ggcggccttc aggccaatgt tcgtcgacca gggacgcatc ggcgccgact ggatcaagga
      901 gcagcaggag atcttccaga agcaggtcga gggcgacgag aagatggtcg gcaagcccgc
      961 caaggtcatc gagggcatcc tcgccggcaa gcttaagaag ctgatgagcg agatctgcct
     1021 ggtcgaccag ggcttcgtca aggacgagaa gcagaccgtg gccgcggtga tggcctcgat
     1081 cgccaaggcc gccggaacgc agctgtcgat cgtcgactac gcctacatca aggtcgggga
     1141 caaccagtaa ggtccatggc cgcccccagc ctcgcggccg gggcggcttc gtcatgagcg
     1201 gatccggcac cggatcctcg aaagggagcg caatggaaga catcaagaac gcgtgcgaag
     1261 agcgcatgaa gaaaaccgta gcctcgctca aggacgattt cgcggctatt cgcaccggcc
     1321 gcgcctcgcc gtccatcctc gacaagcttc gcgtcgacta ctacggccag aaatcgccga
     1381 tatcgcaggt agcgaccgta tccgtacccg aggctaggct catcgtcgtc cagccttggg
     1441 atcgggcgct gatcgtcgag atcgagaagg ccatccagaa gtccgagctc ggcctcaacc
     1501 cgtcgaacga cggcaaggtg atacgcatcg ccatcccccc gctcaccgag cagcgccgca
     1561 aggagctcgt caagagcgcc aagtccagtg ccgaaacctc gcgggtaggc atccgcaaca
     1621 tcaggcggga cggactcgag gacatcaaga aggccggagc tcccgaggac gaggtcaaga
     1681 aggccgagga cgagcttcag aagctgaccg actcgttcat cgcccagatt aacaaggttc
     1741 tcgaggataa agagaaggaa atcctggaag tctgagtatg cagcctacac tagcccgatc
     1801 agttccggcg cacgtcgccg ttttcatgga cggcaacggg cgttgggcca aagctcgagg
     1861 cttgccgagg acggatggcc atcgcgaggg gctcaaggcg gctaagcgcg tcgtgaaggc
     1921 cgccaaggat gccggcgtcc gctacctgac gttgtatacc ttcagtaccg agaattggaa
     1981 acgtgctcag gaagaggtta aattcctcat gggcctggtc agcacctacc tctgcgccga
     2041 gctcgacttc tatcgcgaga acggagtgcg ggtcgtgcat tccggcgacg acgaaggcct
     2101 gccgaaggac gtgcgcgagg cgctcgagcg cgtatcggac gacaccgcgg gcttcgac
//
LOCUS       DAOPHV010000487         2155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_72347_length_2155_cov_3.792381, whole genome shotgun sequence.
ACCESSION   DAOPHV010000487 DAOPHV010000000
VERSION     DAOPHV010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72347_length_2155_cov_3.792381"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /locus_tag="PLQ29_11200"
     CDS             <1..1472
                     /locus_tag="PLQ29_11200"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPG87253.1"
                     /translation="RKVAYEPYFSSRPGWAEQDPELWWRSLVAASSGLVAELPGGADR
                     IAALAVTTQRDTIVCLDGDLRPLRPAILWLDARRAPAPYHPSALVALGVSLVGMREAL
                     QKTQEAGKCNWIMANEPGLWERTRACVLVSGFLNARLSGELADSTASQIGHLPFDYRR
                     QAWAAPGSRNARMFPIAPEKLPRLAPAGTELGRLSARAAAELGLPAGLPVMAAGSDKG
                     CETIGSGVVDETAASLSFGTTATVQTTSRRYREPIAFMPPYPAAIPGRYNPEVEIFRG
                     YWMISWFKSQFAYAEVLEAESRCMAPEAMLDELLRSTPPGGHGLVMQPYWTAGLSSPS
                     AKGAIIGFGDVHERSHLYRAIVEGLAYGLKEGLEAIERASGTRVRSLRVSGGASQSDA
                     ICQASADVLDLPMERGRTCEASGLGAAMCAAAGLGWYPGVEAASAAMSSSSRVFEPDG
                     VNAELYAELYRRVYKRMYRALGPLYESIRDITGYPERAV"
     gene            1484..>2155
                     /locus_tag="PLQ29_11205"
     CDS             1484..>2155
                     /locus_tag="PLQ29_11205"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG87254.1"
                     /translation="MAKQDDFRPDWFEGELPVRSFRSILKWGDPNEYKHPNRKLYALM
                     KETFGLDDEWFKKRRDEGLGVVPESSPCRLAPEHVAALTAIVGPENAHLDTYNRLRIA
                     YGKTMVDLMRLRYGVIEHLPDMALWPRGRDDLRAIVDYCEANAIPVYVYGGGSSVTRG
                     VEPFSGGVSLDMRVHLKRVIKFDERNQTITVEAGMDGPELERLLRNAPETLGATGRYT
                     AGHLPQ"
BASE COUNT          335 a          741 c          768 g          311 t
ORIGIN      
        1 ggcgtaaggt ggcatacgaa ccgtacttca gctcgcgccc cggttgggcc gagcaggacc
       61 ccgagctgtg gtggcgatcg ctcgtcgcgg cctctagcgg gctcgtcgcc gaactacctg
      121 gcggcgccga ccggatcgcg gccctggcgg tgacgaccca gcgcgacacg atcgtctgcc
      181 tggacgggga cctccgcccc ctgcgcccgg ccatcctgtg gctcgacgcg cgcagggcgc
      241 cggcgccgta ccatccatcg gccctggtgg ccctcggcgt gtcgctcgtc ggcatgcgcg
      301 aggctctcca aaagacgcag gaagccggca agtgcaactg gatcatggcc aacgagccgg
      361 ggctctggga acggacgcgg gcctgcgtgc tggtctccgg tttcctgaac gcgaggctct
      421 cgggcgagct ggcggactcg accgcgtcgc agatcggcca cctgcccttc gattaccggc
      481 ggcaggcctg ggccgccccg ggtagccgca acgcgaggat gttcccgata gcgccggaga
      541 agctgccgcg cctcgcgccc gccggtacgg aactcgggcg cctgtcggcc cgggcggcgg
      601 ccgagctcgg gctgcccgcc ggcctgccgg tgatggcggc cggtagcgac aagggctgcg
      661 agacgatagg ctcgggcgtg gtcgacgaga cggccgcgag cctctccttc ggcacgacgg
      721 ctaccgtgca gacgaccagc cgccgctacc gcgagccgat agccttcatg ccgccgtacc
      781 cggcagccat cccgggccgc tacaacccgg aggtcgagat tttccgcggc tactggatga
      841 tatcgtggtt caagagccag ttcgcctacg cggaggtact cgaggccgag agccgctgca
      901 tggcgcccga ggcgatgctc gacgagctcc tgcgctcgac gccgccgggc ggccacggcc
      961 tcgtgatgca gccttactgg acggccggcc tgtcgtcgcc ctcggcgaaa ggcgccatca
     1021 taggcttcgg cgacgtgcac gagcgatcgc acctgtaccg cgccatcgtc gagggcctgg
     1081 cctacggcct caaggagggc ctcgaggcga tagagcgggc gtccgggacg agggtgcgct
     1141 cgctgcgggt ctcgggcggc gccagccaga gcgacgcgat ctgccaagcc tcggccgacg
     1201 tgctcgacct gccgatggag cgggggcgca cctgcgaggc ctcgggcctc ggggcggcga
     1261 tgtgcgccgc cgccgggctg ggctggtatc cgggcgtgga ggcggcgagc gcggccatgt
     1321 cgtcgtcgtc ccgcgtcttc gagcctgacg gggtcaacgc ggagctatac gccgagctgt
     1381 accgccgcgt ctacaagcgc atgtatcggg ccctcgggcc cctgtacgag agcatcaggg
     1441 atatcaccgg ataccccgag cgggccgtct aaaggaggac gatgtggcta agcaagacga
     1501 cttcaggccg gattggttcg agggcgaact gcccgtgcgc agtttccggt ccatcctcaa
     1561 gtggggcgac ccgaacgagt acaagcaccc gaaccgcaag ctgtacgcgc tgatgaagga
     1621 gaccttcggc ctcgacgacg agtggttcaa gaagcggcgc gacgagggcc tcggcgtcgt
     1681 acccgagtcg tcgccctgcc gcctggcccc tgagcacgtc gcggcgctga ccgccatcgt
     1741 gggtcccgag aacgcccatc tcgataccta taaccggctg cggatagcct acggcaagac
     1801 gatggtcgac ctgatgcgcc tgcgctacgg cgtgatcgag cacctgccgg acatggccct
     1861 atggccgcgc ggccgcgacg acctgcgcgc catcgtcgac tactgcgagg cgaacgcgat
     1921 accggtctac gtctacggcg gcggctcgtc ggttacgcga ggcgtcgagc cgttctcggg
     1981 cggcgtcagc ctggacatgc gcgtccacct gaagcgcgtg atcaagttcg acgagaggaa
     2041 ccagacgatc accgtcgagg ccggcatgga cggcccggag ctggagcgtt tgctcaggaa
     2101 cgcgcccgag acgctcgggg cgacgggacg ctacaccgcc ggccacctgc cgcag
//
LOCUS       DAOPHV010000488         2146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_72775_length_2146_cov_4.701100, whole genome shotgun sequence.
ACCESSION   DAOPHV010000488 DAOPHV010000000
VERSION     DAOPHV010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72775_length_2146_cov_4.701100"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1684)
                     /locus_tag="PLQ29_11210"
     CDS             complement(<1..1684)
                     /locus_tag="PLQ29_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87255.1"
                     /translation="MHRTLALVFATAVFVSAASADLFSPDRPLSTVETEYFTFIYAAE
                     SEPAVRYLASFADDAYREIAALLGTSPRARLPVVMTPDSETLNGYYTAYPYPRIVLYQ
                     APTEAGSSLGSFDDDLRKLFQHELTHAVSLSLRGGAEGALVAVFGSPLGLSTYLAPRA
                     FVEGVTVSFESMNGFGRATDPLAGAIIRQDIVEGRGRSFAQACGADDRYPSSALHYLY
                     GGYFSRYLQGRWGIGKYAELWTLFGARALLRPLDDSWLGEGRFSRVYGISLSEAWTDF
                     MRSMAPTAPVYMAAEPRTDASSVSALCSSGDALYYADEAAETVYRLDPVSGDSRPLFR
                     AGAEVSRLDASPDGSALLVSTTRLAGGFPKLAVMEYTVGGRGGPRLLPLSRVRDAAYL
                     PGGGLVGVSVDGYETDLVVARGEARSTLLEGNERTNFSSPVASADGAYVYAIARLDGV
                     PSIIRLALDGTGTRADSVERLVVPDGLSWLRYLSMGTDGVLRSGWDDGSFYRLVELDD
                     GLIRYQSVPISGGVHSPVLSAGLVRYIGRFSDGQRLCDYPTDRTPLGFAEAPA"
     gene            complement(1797..>2146)
                     /locus_tag="PLQ29_11215"
     CDS             complement(1797..>2146)
                     /locus_tag="PLQ29_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87256.1"
                     /translation="SEMKNATGELSIGAKQILEALGSLVSTTEEVKGSSVDMKARVTA
                     IVEAMRSVAAISSDTKYGMEEITIGIDEIYKAAESISRAGGENSESVATLKALVERFT
                     VSMAMEGSSTIER"
BASE COUNT          369 a          710 c          758 g          309 t
ORIGIN      
        1 gcgccggagc ctcggcgaag ccgagaggcg tgcggtctgt cgggtaatcg cagaggcgct
       61 ggccgtcgga gaaacggccg atatagcgca ccaggccggc ggacagtacc ggactgtgga
      121 cgccgcccga gatcgggacg ctttggtacc ggatcagccc gtcgtcgagc tcgacgaggc
      181 gatagaacga accgtcatcc cagccggaac gtaggacgcc gtccgtgccc atcgatagat
      241 agcgcagcca ggaaaggccg tcggggacga cgagacgctc taccgagtcc gcgcgggtcc
      301 cggtcccgtc caacgctagc ctgatgatcg aggggacgcc gtcgagcctg gcgatcgcgt
      361 agacgtaggc tccgtcggcg gaggccacgg gcgacgagaa attcgtacgc tcgttgccct
      421 cgagcagcgt agagcgggcc tcgccgcgcg cgacgaccag gtcggtctcg tagccgtcga
      481 cggacacccc gaccagcccg ccgccgggta ggtaggccgc gtccctgacg cggctcaggg
      541 gcaggagccg agggccgcct cggccgccga ccgtgtactc catgacagcg agcttgggga
      601 aaccgcctgc gaggcgggtc gtggagacga ggagggccga gccgtccggc gaggcatcga
      661 gccgcgacac ctcggcgccg gccctgaaca gcgggcggct atcgccggag accgggtcga
      721 gccggtagac ggtctcggcc gcctcgtcgg catagtagag cgcgtcgccc gacgagcaga
      781 gcgccgagac ggacgacgcg tccgtccgag gctcggcggc catgtagacg ggcgccgtcg
      841 gcgccatgga gcgcatgaag tcggtccagg cctccgatag cgatatgccg tagacccgcg
      901 agaagcgccc ctcgccaagc caactgtcgt cgagcggtcg caggagcgcc ctagcgccga
      961 agagcgtcca gagctcggcg tacttcccaa tgccccagcg cccctgcagg tagcgcgaga
     1021 agtagccgcc atagaggtag tggagcgcgc tcgatgggta gcggtcgtcg gctccgcagg
     1081 cctgggcgaa gctcctgccc cggccttcga cgatgtcctg gcgtatgatg gcgccggcga
     1141 gcgggtcggt cgcacggccg aagccgttca tgctctcgaa cgacacggtg acgccctcga
     1201 cgaaagccct gggcgctaga tacgtcgaca ggccgagcgg cgagccgaag acggcgacga
     1261 gcgcgccctc cgcgcccccc cggaggctta gcgacaccgc gtgcgtgagc tcgtgctgga
     1321 acagctttct cagatcgtcg tcgaacgatc cgaggctcga gcctgcctcg gtgggggcct
     1381 ggtacagcac gatgcgcgga tagggatagg cggtgtagta gccgttcagc gtctcggaat
     1441 cgggcgtcat gacgaccggc agcctcgcgc gggggctcgt gccgagcagc gcggctatct
     1501 cgcggtacgc gtcgtcggcg aaggaagcaa ggtaccgtac cgccggctcc gactcggccg
     1561 cgtagatgaa cgtgaagtac tcggtctcga ctgtggacaa aggcctgtcg ggcgagaaca
     1621 ggtcggcgga cgccgcggag acgaagaccg cggtcgcgaa cactaaggcg agcgtccgat
     1681 gcatgcatac ccccggtgcg acgaggcgcc gccgcccgtg gagtatacgg acaaaacggc
     1741 cggaggcgca acatccgtcg cgcctccggg ccggcgccgc agcgcgccgg ccgtccttat
     1801 cgttctatcg tcgaactacc ttccatggcc atggagaccg tgaaacgctc gacgagagcc
     1861 ttgagcgtcg ccacgctctc ggaattctcc cctccggccc gcgagatgga ctcggcggcc
     1921 ttgtatatct cgtcgatgcc gatggtgatc tcctccatgc cgtacttcgt gtcgctcgag
     1981 atggccgcga cgctgcgcat cgcctcgacg atggccgtga cgcgggcctt catgtcgacc
     2041 gacgagccct tgacctcctc ggtggtcgat acgagcgagc cgagggcctc gagtatctgc
     2101 ttggcgccga tggacagctc gccggtggcg ttcttcatct ccgaca
//
LOCUS       DAOPHV010000489         2143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_72927_length_2143_cov_3.267720, whole genome shotgun sequence.
ACCESSION   DAOPHV010000489 DAOPHV010000000
VERSION     DAOPHV010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72927_length_2143_cov_3.267720"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..320
                     /locus_tag="PLQ29_11220"
     CDS             66..320
                     /locus_tag="PLQ29_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPG87257.1"
                     /translation="MQAAPVVIAICMGSSCYARGNARNAELIKAWLAREGRDARIEFN
                     GTLCEGLCKEGPNLRIGDRVYRGVDPAAVEDILEHEFGER"
     gene            323..2104
                     /locus_tag="PLQ29_11225"
     CDS             323..2104
                     /locus_tag="PLQ29_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPG87258.1"
                     /translation="MPNYEPIRTESAACQDCYKCVRECPVKAIRVCEGKASVVPELCV
                     LCGHCVEVCPAEAKKVRSDVERAKALIGIKPTVVASLAPSFASEFPELEPARLISSLT
                     SLGFALVSETARGADSVSRSLAATLSGAKDSGPGRVILSSACPVVNDYLLLYRPELTG
                     FLSPLASPLVAHAAELKASLGPGAAVVFIGPCIGKKREADRSAGAVDAAVTFKELRAW
                     FEQEGLDPYAAPITGNERFYPDKAAKGSLYPVDGGMIAGVKKYAPGLAAHCVSYSGLG
                     QIERALDGLEAYDGRSPIFLELLACEGGCVNGPEAQHRAGSVRKRLAVLDYARSSADR
                     RDAQSAPVAERVAEPVANESPSEESVSEALRSIGKTRPEHELNCSSCGYDSCRAFAAA
                     MISGKAERTMCVSYMRQLAQKKANALIKAMPSGVVLVDTDLRVVECNRPFARLLGPEV
                     EAMFDAKPGLEGADLRRLVPFWQLFEAAFADDGLSEPANGNRRESGPRDVKFDGRVLS
                     GSIFAVEPGLVVGGIFQDVTVPWIRRDRVVSQAKAVIRKNVATVQKIAYLLGENAAES
                     ETILQSIIESFDAGDHGPESRDRQKRG"
BASE COUNT          348 a          767 c          686 g          342 t
ORIGIN      
        1 gtccgtagcc gcgctcgccc ggtctttcga ttgacattat cgatatattt atatattatt
       61 agtccatgca agcagccccc gtcgtcatcg ccatctgtat gggatcatcg tgctatgcca
      121 ggggcaacgc ccgcaacgcc gagctgatca aggcctggtt ggcgcgcgag ggccgcgacg
      181 ctagaatcga attcaacggg accctctgcg agggcctctg caaggaaggc cccaacctgc
      241 gcatcggcga ccgcgtctac cgcggggtgg accccgcggc ggtggaagac atcctggagc
      301 acgaattcgg ggagcgttga ccatgcccaa ctacgaaccc atccgcaccg agagcgcggc
      361 ctgccaggac tgctacaagt gcgtgcgcga gtgtccggtc aaggccatca gggtctgcga
      421 gggcaaggcc agcgtcgtgc ccgagctgtg cgtgctctgc ggccactgcg tcgaggtctg
      481 cccagccgaa gccaagaagg tgcggtcgga cgtggagcgc gccaaggcgt tgatcggcat
      541 caagccgacc gtggtcgcga gcctcgctcc gtccttcgcg tcggaattcc ctgagctcga
      601 acccgccagg ctgatctcgt cgcttacctc gctcggcttc gccctcgtgt cggagaccgc
      661 gcgcggggcc gactcggtat cgcgttccct ggccgccacc ctctcgggcg ctaaggactc
      721 cggccccggc cgcgtgatcc tttcgagcgc ctgccccgtc gtcaacgact acttactcct
      781 ctacaggccg gagctgaccg gcttcctgtc gccgctcgcc tccccgctcg tcgcccacgc
      841 cgccgagctc aaagcgtcgc tcggccccgg cgccgccgtc gtgttcatcg gcccgtgcat
      901 cggcaagaag cgcgaggcag accgttccgc cggcgcggtc gacgcggccg tcaccttcaa
      961 ggaactgcgc gcctggttcg agcaggaggg cctcgacccc tatgccgccc cgataaccgg
     1021 aaacgagcgt ttctaccccg acaaggcggc gaagggctcg ctctacccgg tggacggcgg
     1081 catgatagcc ggcgtcaaga agtacgcgcc cggcttggcg gcccactgcg tctcgtactc
     1141 cggcctcggg cagatcgagc gcgccctgga cggcctcgag gcctacgacg gacgctcgcc
     1201 catcttcctc gagctgctcg cctgcgaagg cggctgcgtc aacgggcccg aggcccagca
     1261 ccgcgccggc tcggtacgca agcgcctcgc ggtactcgac tacgcccgct cctcggccga
     1321 tcgccgcgac gcgcaatctg cccccgtcgc cgagagggtg gccgagcctg tagccaatga
     1381 aagcccctcc gaggagtccg tgtccgaggc gctccgctcg ataggcaaga cccggcccga
     1441 gcacgaactc aactgctcgt cctgcggcta cgacagctgc cgggccttcg ccgccgcgat
     1501 gatctccggc aaggccgagc gcacgatgtg cgtgtcgtac atgcgccagc ttgcgcagaa
     1561 gaaggccaac gcgctgatca aggccatgcc gtccggggtc gtgctcgtcg ataccgacct
     1621 ccgggtcgtc gagtgcaacc ggcccttcgc caggctactc gggcccgagg tcgaggccat
     1681 gttcgacgcc aagccggggc tcgagggcgc cgacctccgc aggctcgtgc cgttctggca
     1741 gctcttcgag gcggccttcg ccgacgacgg gctatcggag ccggccaacg gcaaccgtcg
     1801 cgaatcgggt cctcgcgacg tcaaattcga cggccgcgtg ctgtcgggct cgatcttcgc
     1861 ggtcgagccg gggctcgtcg tcggcggcat cttccaggac gtgaccgtgc cgtggatacg
     1921 ccgggatcgg gtcgtctccc aggccaaggc cgtgatacgc aagaacgtgg ccaccgtgca
     1981 gaagatagcc tacctgctcg gggagaacgc cgccgagagc gagaccatac tccagtcgat
     2041 catcgagtcg ttcgacgccg gcgaccacgg ccccgagagc cgcgaccgcc agaagcgcgg
     2101 ctagccggcc atgaaggact ccttcctcga gctatcgcat cac
//
LOCUS       DAOPHV010000490         2140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_73067_length_2140_cov_3.060911, whole genome shotgun sequence.
ACCESSION   DAOPHV010000490 DAOPHV010000000
VERSION     DAOPHV010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73067_length_2140_cov_3.060911"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PLQ29_11230"
     CDS             complement(<1..556)
                     /locus_tag="PLQ29_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPG87259.1"
                     /translation="MNKIVSKARLSDEVFRIEVEAPNIARERKAGQFVIVQTDTEWGE
                     RVPLTIADADPQRGTVTLIFQTVGVSTHKLAELEPGQAIEAILGPLGNPTHIERFGRA
                     VCVGGGIGVAPMHPIAQALKQAGNKVTIIIGARTRDLVILEDEMRAIADELIVVTDDG
                     SYGRKALVTEPLKELCESATPPDLV"
     gene            815..>2140
                     /locus_tag="PLQ29_11235"
     CDS             815..>2140
                     /locus_tag="PLQ29_11235"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HPG87260.1"
                     /translation="MVRHSAQRLVAPVCIALLSLAAACRSPILGAPDATGTLTIVLPA
                     ASASRPASRAASRAPGGLASLAGTVTRWVLEGAGPGGHSDSWEFDAASMSAAGNTVSL
                     ELLVGDWTITVRGYEADGGEAFTATRTVTVARDGENVVQLTLEWAEKALVARFSFDSA
                     IEDDKRRLAATPGNLYSSDPVIAASSDLTEPAVDGLAVAYIADADPEDYGAYIDIAAA
                     DGSGLELGQSFALSMWAYPTTESEDSDRVLITSQADPSADSPGFTVSVNAGLVTLSCS
                     DGTATGATTRDGYEYGAWNHIAISIDGESGYASIYVNGAPNAGPLAFSGIPSDPTIRI
                     GARMTNSGFFQGSFDDLRVYAGALAADEIAAIRADGYFSGGRGTAASPYLVATPMDLY
                     NVRRFPAAHFRQTADIDLSASAVTSEWSPISSFTGNYDGSGRSVSNVALG"
BASE COUNT          354 a          746 c          657 g          383 t
ORIGIN      
        1 cgacgaggtc cggcggggta gcggactcgc acagctcctt gagcggctcg gtgacgaggg
       61 ccttgcggcc gtagctaccg tcatcggtca cgacgatcag ctcgtcggcg atggcgcgca
      121 tctcgtcctc gagtatcacc aggtcgcggg tcctcgcgcc gatgatgata gtaaccttgt
      181 tgcccgcctg cttcagggcc tgggctatgg ggtgcatcgg cgccacgccg ataccgccgc
      241 cgacgcagac ggcgcgcccg aagcgctcga tatgcgtcgg gttccccagc ggcccgagta
      301 tcgcctcgat ggcctggccc ggttcgagct cggcgagctt gtgcgtgctg acgccgaccg
      361 tctggaagat cagcgtgacg gtaccgcgct gcgggtcggc gtcggctatg gtcaagggga
      421 cgcgctcgcc ccactcggta tcggtctgga cgatcacgaa ctgcccggcc ttgcgctcgc
      481 gggctatgtt cggggcctcc acctcgatgc ggaatacctc atcggacagg cgggccttcg
      541 atactatctt gttcaccctt cctcctcttc gctaccggaa acgccaccat aatagagcat
      601 agcgctatcc tcgtcgagcg tttcacgtct tgacgccggc ccatgcccgc gtttcgccac
      661 attgtataac tatatttgta tcgatatcaa gactacgata caaccaaacc gacgattcgt
      721 cgctccggcg cttggtttat taggcagctc gtgataggtt ctttcacgta tcgacgatct
      781 aatcgataga aaattcatca ctgggagacc gcccatggta cgccactccg cccagcgcct
      841 cgtcgcgccc gtctgtatcg ccctgctatc gctcgcggcc gcttgccgct cgccgatact
      901 cggcgccccg gacgccacgg gcaccctgac gatagtcctg cctgcagcct ccgcttcgcg
      961 ccccgcttcg cgcgccgcct cgcgcgcacc gggaggcctc gcgagcctgg ccggcaccgt
     1021 gacccgctgg gtgctcgaag gtgccggccc cggcggccat tccgatagct gggagttcga
     1081 tgccgcgagc atgtccgccg cgggcaacac cgtgagcctc gagttgctcg tcggcgactg
     1141 gaccatcacc gtacgcggct acgaagccga cggcggcgag gcattcaccg ccacccgtac
     1201 cgtaacggtc gcccgtgacg gagagaacgt cgtgcaactg accctggagt gggccgagaa
     1261 ggcgctggta gcgcgcttca gcttcgacag cgctatcgaa gacgataagc gcaggctcgc
     1321 ggcaacgccg gggaacctct actcgagcga tccggtcatc gccgcctcct ccgacctcac
     1381 ggagccggcc gtcgacggat tggccgtcgc ctatatcgcc gacgccgacc ctgaagacta
     1441 cggcgcgtac atagacatcg cggccgcgga cggcagcggc ttggagctcg gacaatcctt
     1501 cgccctgtcc atgtgggcct atcccaccac ggagtccgag gatagcgatc gcgtcctaat
     1561 cacgagccag gccgacccgt cggccgactc gccaggattc acggtctcgg tgaacgcggg
     1621 cctggtcacg ctctcgtgct cggacggtac ggcgaccggc gcgaccacac gggacggcta
     1681 cgaatacggc gcgtggaacc atatcgctat ctctatcgac ggcgaatcgg gctatgcctc
     1741 gatctacgtg aacggcgcgc cgaacgccgg cccgctagcc ttttctggca ttccaagcga
     1801 tccgactata cgtatcggcg cgcgcatgac gaactcgggt ttcttccagg gctcgttcga
     1861 cgacctccgc gtctacgcgg gcgccctagc ggccgacgag atcgcggcca tccgcgccga
     1921 cggctacttc tccggcggac gaggtacggc cgcctcgccc tacctcgtcg cgacgccaat
     1981 ggacctgtac aacgtgcgcc gcttccccgc ggcccacttc aggcagacgg cggacattga
     2041 cctgagcgcg agtgccgtca cgagcgagtg gtcgcccatc tcgtccttca ccggaaacta
     2101 tgacgggagc gggaggagcg tctcgaacgt cgcgctcggc
//
LOCUS       DAOPHV010000491         2136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_73240_length_2136_cov_3.237386, whole genome shotgun sequence.
ACCESSION   DAOPHV010000491 DAOPHV010000000
VERSION     DAOPHV010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73240_length_2136_cov_3.237386"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1205)
                     /gene="mglC"
                     /locus_tag="PLQ29_11240"
     CDS             complement(159..1205)
                     /gene="mglC"
                     /locus_tag="PLQ29_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HPG87261.1"
                     /translation="MATQEPTALGERLNRRAVNDFMLKYAIYFVFVILIIAITVKEPS
                     FMSIENFRNILSMSATRIIIAMGVGGILITGGTDLSAGRQVGLAAVVSSSMLQALDYP
                     RRMYPELGQLPLIVPILAAMLICGAIGLLNGLVVSKLRVPPFIATLGSMVAVYGATSL
                     YFDRPPYGAQPIGGLDRRFTNLGSGYIGLDGTYSIPFIVLIALGIALVVWVLHNKTTF
                     GKNIYAIGGNPEAAKVSGVNVTKNLLIAYTIAGICYGLAGSLEAARTGGATNNYGNGY
                     ELDAIAACVVGGVSTSGGIGTVPGMLVGVLIFSVINYGLTFVNMSPYWQQIVKGAIII
                     AAVALDIRKYLNKR"
     gene            complement(1217..>2136)
                     /locus_tag="PLQ29_11245"
     CDS             complement(1217..>2136)
                     /locus_tag="PLQ29_11245"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPG87262.1"
                     /translation="IVYISHKMEEILEIADEVSIMRDGKHVGTWPSAELTTDVIIKRM
                     VGRDLTHRFPPRNNVPGEVVLKVENFTSAERNSFKDVSFELRRGEILGIGGLVGAKRT
                     ELVESIFGLRFVDSGAITLRGKEIKIKSPVQAMQVGFSLLTEERRATGIVPMLGVKEN
                     MLLANLRRYVGRTGLINEKRGVDDVRKKIADLRIKTPSHRTLIKDLSGGNQQKVLFAK
                     WLLTEPEILILDEPTRGIDVGAKYEIYTIIAELAASGKSIIMISSEMPELIGMSDRIM
                     VMCAGRKTGELDGKTATQEEIMRLAALFE"
BASE COUNT          364 a          648 c          707 g          417 t
ORIGIN      
        1 gagatccgcg gcgtctgccc cgactgcgcc gccaaggacg ccgagtagcg ccgcgcccct
       61 ccttaaaaga cgcggcgctc cgtctccggg gcgccgctga aaggaggtag aaacgtatct
      121 tcgccgcatc ccacgggccc gggggcccgc gtatcggatt agcgcttgtt caggtacttg
      181 cggatatcga gggccaccgc cgcgataatg atcgctccct tgactatctg ctgccagtac
      241 ggggacatgt tcacgaaggt caggccgtag ttgatcacgc tgaagatcag cacgccgacg
      301 agcatgcccg gtacggtgcc gatgcctccg gaggtcgata cgccgccgac cacgcaggcc
      361 gcgatggcgt cgagctcata gccgttgccg tagttgttcg tcgcgccgcc ggtgcgcgcg
      421 gcctccaggg aacctgccag gccgtagcag atgccggcga tcgtgtaggc tatgagcagg
      481 ttcttcgtca cgttgacgcc ggacaccttg gccgcctctg gattgccgcc gatggcgtag
      541 atgttcttgc cgaaggtcgt cttgttgtgc agcacccaga cgaccagcgc gatgccgagg
      601 gctataagca cgatgaacgg tatggagtag gtgccgtcga gtccgatata gccggagccg
      661 aggttcgtga agcggcggtc gaggccgccg atcggctgag ctccgtaggg aggccgatcg
      721 aagtacagcg aggtggcgcc gtagacggcg accatcgagc ctagggtggc gatgaacggc
      781 ggtacgcgca gcttcgacac gacgaggccg ttgagcaggc cgatcgcgcc gcagatgagc
      841 atcgccgcca ggatcggtac gatcaggggc agctggccga gctcggggta catgcgacgc
      901 gggtagtcga gggcctgcaa catggagctc gatacgaccg ccgccaggcc gacctgacgg
      961 ccggcggaca ggtccgtgcc gccggtgatc aggatgccgc cgacgcccat cgctatgatg
     1021 atgcgggtgg ccgacatcga gaggatgttc ctgaaattct cgatcgacat gaacgacggt
     1081 tccttgaccg tgatggcgat gatcaggatc acgaagacga agtagatcgc gtacttcagc
     1141 atgaagtcgt tgacggcccg cctgttcagc cgttcgccga gcgccgtggg ttcttgtgtt
     1201 gccatcgagt tctcccttat tcgaacagcg ccgccaggcg catgatttct tcctgagtcg
     1261 cggtcttgcc gtcgagctcg ccggtcttcc tgccggcgca catgaccatg atcctgtccg
     1321 acatgccgat cagctccggc atctcggagc tgatcatgat gatgctcttg ccgctggccg
     1381 ccagctcggc gatgatcgtg tagatttcgt acttggcgcc tacgtcgatg cctcgggtcg
     1441 gttcgtcgag tatcaggatc tcgggctccg tcaggagcca cttggcgaat aggaccttct
     1501 gctggttgcc gccggacagg tccttgatca gggtccggtg gctcggggtc ttgatgcgca
     1561 gatcggctat cttcttcctc acgtcgtcga cgccgcgctt ctcgttgatc aagccggtcc
     1621 tgccgacgta gcggcgcagg ttggcgagca acatgttctc cttgacgccg agcatcggga
     1681 cgatgccggt ggcgcgccgc tcctcggtca gcagggagaa gccgacctgc atggcctgga
     1741 cgggcgactt gatcttgatc tccttgccgc ggagcgtgat ggcgcccgag tcgacgaagc
     1801 gcaggccgaa gatagactcg acgagctcgg tgcgcttggc gccgacgagg ccgcctatgc
     1861 cgaggatctc gccccttcgc agctcgaagc tgacgtcctt gaacgagttg cgctccgcgg
     1921 aggtgaaatt ctcgaccttg agcacgacct cgcccggcac gttgttccgg ggcgggaagc
     1981 ggtgcgtcag gtcgcggccc accatgcgct tgatgatgac gtcggtggtc agctcggcgg
     2041 atggccaggt gccgacgtgc ttgccgtcgc gcattataga aacctcgtcg gctatctcga
     2101 ggatctcttc catcttgtgc gatatataga ctatcg
//
LOCUS       DAOPHV010000492         2136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_73253_length_2136_cov_2.768381, whole genome shotgun sequence.
ACCESSION   DAOPHV010000492 DAOPHV010000000
VERSION     DAOPHV010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73253_length_2136_cov_2.768381"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..541
                     /gene="dtd"
                     /locus_tag="PLQ29_11250"
     CDS             92..541
                     /gene="dtd"
                     /locus_tag="PLQ29_11250"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPG87263.1"
                     /translation="MRAVVQRVRNASISVDGTTVGAIGAGLLVYLGVGKGDGEADAKW
                     LAEKICGLRIFEDSGGKMNLSVVDTGGGVLSVSQFTLLADTRKGKRPSYNDAAEPAVA
                     EALYERFKEFVAAIVPTTASGVFGASMEVAYTNMGPVTILLDTKQPF"
     gene            complement(657..1382)
                     /locus_tag="PLQ29_11255"
     CDS             complement(657..1382)
                     /locus_tag="PLQ29_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HPG87264.1"
                     /translation="MVELVDALERLLTAYGPRGWWPLPSLAGTPGRDGTGYALGPSLP
                     VGSGGGANERSLRFEIAVGAVLAQNTAWAGAERAVATLVARSSAAGLPLSPQLLRSLA
                     GGELEDMLRPAGTFRIKAGYLRSLASAWSELDEGVPTRDGLLALPGIGFETADCVLLY
                     AYGVPAFVADAYARRLLSRLGYALDSSSYEGARHYAEERLPHEAPFLAEAHALIVEHC
                     KLRCRSRPLCDGCPLATVCDHCR"
     gene            complement(1566..>2136)
                     /locus_tag="PLQ29_11260"
     CDS             complement(1566..>2136)
                     /locus_tag="PLQ29_11260"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968761.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPG87265.1"
                     /translation="AAVECAKKKIPVSPEIMIPLICDPKELEVLRARTQAIVDAAISK
                     AGLKMKVLIGTMIEVPRAAILADKVAAHADFFSYGTNDLTQMTYAFSRDDAGSFLPEY
                     IRQEVLPSNPTETVDEEGVGFLMKHASDYGRKTKADLKLGICGEQGGDPATIDFCYRI
                     GLNYVSCSPFRVPIARLAGAQAVLRNAKK"
BASE COUNT          372 a          645 c          712 g          407 t
ORIGIN      
        1 cccgtcgagg tgctcgcccg cgtgcgtaac cagggcaagg cgttctacct cgaatgcctg
       61 gctatacacg aacaatgagg aacggagttg tatgcgggcg gtagtgcagc gagtcaggaa
      121 cgcgtctata tcggtcgacg ggacgacggt gggcgccatc ggggccggtc tcctcgtcta
      181 tctgggcgtc ggcaagggcg acggcgaggc cgacgcgaag tggttggctg aaaagatctg
      241 cggcctgcgc atcttcgagg actcgggcgg gaagatgaac ctgtcggtcg tcgacaccgg
      301 cggcggtgta ttgtccgtgt cgcagttcac cctgctcgcg gatacgagga aaggtaagcg
      361 tccgtcgtac aacgacgcgg ccgagccggc cgtcgccgag gccctgtatg agcgcttcaa
      421 ggaattcgtg gcggccatcg taccgactac cgcgtccggc gtcttcggag catcgatgga
      481 ggtggcgtat accaacatgg gcccggtgac gatactgctc gatacgaagc aacccttcta
      541 gcccgccagg ttccgtaata tagcgcgccg caatacgtcg ctccaagctt ctgggtcttc
      601 ttcatcgtta taccacgacg cttctaccgc tgcctctcga gttctcttcg ttctccttat
      661 cggcagtgat cgcagacggt cgcgagcggg caaccgtcgc acagggggcg cgagcggcaa
      721 cggagcttgc agtgctcgac tatgagcgcg tgagcctcgg cgaggaaggg cgcctcgtgc
      781 ggcagacgct cctcggcgta atgcctcgcg ccttcgtacg agctgctatc tagtgcgtag
      841 ccgagccttg acagcagcct gcgcgcgtag gcgtcggcga cgaatgccgg aacgccgtag
      901 gcgtagagca ggacgcagtc ggcggtctcg aagccgatgc cgggtagggc cagaaggccg
      961 tcgcgggtcg gtacgccttc gtcgagctcc gaccaggccg aggctaggct cctcaggtac
     1021 ccggccttta tcctgaaggt gccagccggc ctcagcatgt cctcgagctc gccacccgcc
     1081 aaagagcgga gcagctgtgg tgagaggggc agccccgcgg cagacgagcg ggctaccaag
     1141 gtggctacgg cccgctcggc tccggcccag gccgtgttct gcgcaaggac ggccccgacc
     1201 gctatctcga aacggagcga gcgttcgtta gcgcctccgc cggacccgac gggtaacgac
     1261 gggcccaggg cgtacccggt gccgtcgcgg cctggcgttc cggccaggga cggtagcggc
     1321 caccagcctc ggggtccgta ggctgtgagt aatcgctcca atgcatcgac cagttcaacc
     1381 acgaatatgc tccagttaat aagacgacga agagtatacg agcatacgag gtatgctaga
     1441 gaagacccat tttaccgtta tgctctttcc tttctcttat tcacatatcc tcttcgttcg
     1501 cattagtaaa aaaaggcccg gctatccgtt cggatagccg ggccgttcat cagctcaggg
     1561 tcttcttact tcttggcgtt gcggaggacc gcctgggcgc cggccaacct ggctatcggc
     1621 acgcggaagg gcgagcacga tacatagttg agtccgatgc ggtagcagaa gtcgatcgtc
     1681 gcagggtcgc cgccctgctc cccgcagatg ccgagcttga ggtcggcctt ggtcttgcgg
     1741 ccatagtcgg aggcgtgctt catcaggaag cctacgccct cctcgtcgac ggtctcggtc
     1801 gggttgctcg gtagcacttc ctgcctgatg tactcgggca ggaaggagcc ggcgtcgtcg
     1861 cggctgaacg cgtaggtcat ctgggtcagg tcgttggtgc cgtagctgaa gaagtcggcg
     1921 tgcgcggcga ccttgtcggc gaggatggcg gcgcgcggca cctcgatcat ggtgccgatg
     1981 agcaccttca tcttgaggcc ggccttggag atcgctgcgt cgacgatggc ctgggtccgg
     2041 gccctgagga cctcgagctc cttgggatcg cagatcagag ggatcatgat ctcggggctg
     2101 acggggatct tcttcttggc gcactcgacg gcggcc
//
LOCUS       DAOPHV010000493         2134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_73321_length_2134_cov_4.098124, whole genome shotgun sequence.
ACCESSION   DAOPHV010000493 DAOPHV010000000
VERSION     DAOPHV010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73321_length_2134_cov_4.098124"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1719)
                     /gene="ettA"
                     /locus_tag="PLQ29_11265"
     CDS             complement(40..1719)
                     /gene="ettA"
                     /locus_tag="PLQ29_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HPG87266.1"
                     /translation="MATVDDKKIIYSMYRVSKKHGTKQVLKDVSISYYYGAKIGVIGL
                     NGSGKSSLLRILSGVDTEFAGETTCAPGYSIGFLEQEPHLEPGKTVREVVSEGVQDVV
                     DALAEFEAVSEAFGDPDADFDKLADKQAKLQEKIEALDGWNLDARLDLAMEALRCPPA
                     DQVVDVLSGGERRRVALCRLLLKKPDILLLDEPTNHLDAETVAWLERHLQQYEGTVIA
                     VTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLDQKSKQLELEEKGNSERAKTLERE
                     LEWVRMSPKGRHAKSKARIAQYEKLLADGEREKVRDTKLVITPGPRLGQVVIEAKGLA
                     KSFGDKCLFQGLEFTVPPGAVVGIIGPNGAGKTTLFKMIAGLEQPDGGSLKLGDTVKL
                     AYADQMRAGLDPDKTVWQQLSGGLDIIKLGGVKEVNSRAYCSWFNFSGGDQQKKVGVL
                     SGGERNRLNLAMMLKEGANVILLDEPTNDLDVNTLRALEEALDGFAGSALVVSHDRWF
                     LDRVCTHLLAFEDGEVVWYDGNWSEYAEYRREKLGGDADRPHRYVYRKLER"
     gene            1801..>2134
                     /locus_tag="PLQ29_11270"
     CDS             1801..>2134
                     /locus_tag="PLQ29_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87267.1"
                     /translation="MGIVADGRPAIRVGRFVERELERSYRVYELDSDRRSVSLVVPLL
                     GVVFLGFGALDYITLPRDAALVALLSIRALFFALAVAVPRLVRSSEPLGRRELALGAV
                     TAAGIAAFC"
BASE COUNT          303 a          693 c          705 g          433 t
ORIGIN      
        1 ccgaccgccc gcgggccgga tggtcggcgg ccgcttttcc tatctttcca gcttcctgta
       61 gacgtagcgg tgcgggcggt cggcgtctcc gccgagcttc tcgcggcggt actcggcgta
      121 ctctgaccag ttgccgtcgt accagaccac ctcgccgtcc tcgaaggcca ggagatgggt
      181 gcagacgcga tcgaggaacc agcggtcgtg gctgacgacg agggccgagc ccgcgaaacc
      241 gtcgagggcc tcctcgaggg cgcgtagcgt gttgacgtcc aggtcgttcg tcggctcgtc
      301 gaggaggatc acgttggcgc cctccttgag catcatcgcc aggttaagtc ggttgcgctc
      361 tccgccggat agtacgccga ccttcttctg ctggtcgccg ccggagaagt tgaaccaaga
      421 gcagtaggcc ctggagttca cttccttgac gccgccgagc ttgatgatgt cgaggcctcc
      481 ggacagctgt tgccagacgg tcttatcggg gtcgaggccg gcgcgcatct ggtcggcata
      541 ggccaacttg accgtatcgc cgagtttgag ggagcctccg tcgggctgtt ccaggccggc
      601 tatcatcttg aacagagtcg tcttgccggc gccgttcggc cctatgatgc cgacgacggc
      661 tcccggcggg acggtgaact cgaggccctg gaacaggcac ttgtcgccga acgacttggc
      721 caggcccttg gcctcgatga cgacctgccc gagcctcggg ccgggcgtga tgacgagctt
      781 cgtatctctg accttttcgc gctctccgtc ggccaggagt ttctcgtact gggcgatgcg
      841 cgccttgctc ttcgcgtggc ggcccttcgg gctcatccgc acccactcga gctccctctc
      901 cagggtcttg gcgcgctcgg agttgccctt ctcctcgagc tcgagctgct tggacttctg
      961 gtcgagccag ctcgagtagt tgcccttcca cgggatgccc tcgccgcggt cgagctccag
     1021 gatccagccg gccacattgt ccaggaagta acggtcgtgg gtgacggcga tgaccgtacc
     1081 ctcgtactgc tgcagatggc gctcgagcca cgcgaccgtc tcggcgtcca ggtggttagt
     1141 cggctcgtcg aggaggagta tgtcgggctt cttgaggagg aggcggcaca gcgcgacgcg
     1201 tcggcgttct cctccggata gcacgtcgac gacctggtcg gccggcgggc agcgcaaggc
     1261 ctccatcgcc aggtcgaggc gcgcgtcgag gttccagccg tcgagggcct cgatcttctc
     1321 ctgcagcttg gcctgcttgt cggccagctt gtcgaagtcg gcgtcggggt cgccgaaggc
     1381 ctcgctcacg gcctcgaact cggccagggc gtccacgacg tcctgtacgc cctcggagac
     1441 gacctcgcgc acggttttac ccggctccag atggggttcc tgctcgagga agccgatcga
     1501 gtagccgggc gcgcacgtgg tctcgccggc gaattcggta tcgacgccgg acaggatgcg
     1561 taggaggctc gacttgcccg agccgttcag tcctatcacg cctatcttgg cgccgtagta
     1621 atagctgatg gatacgtcct tgaggacctg cttggtaccg tgcttcttgg agacgcggta
     1681 catcgaatag atgatcttct tgtcgtcgac tgtggccatg cgcctagtat agcgaaaacg
     1741 accgcgttcg ttgagggggg cgatagaccc tggcggcgcg cgatgataca gtacggctat
     1801 atgggaatag tagccgacgg caggccggcc atacgcgtcg gccggttcgt tgaacgggag
     1861 cttgaacgat cctaccgcgt ctacgagctc gactccgata ggcggagcgt cagcctcgtc
     1921 gtgccgctcc tgggcgtcgt cttcctcggt ttcggagcgc tcgactatat taccctgccg
     1981 cgcgacgcgg ccttagtcgc cctcctctcg atccgggcgc tgttcttcgc gctggccgtg
     2041 gccgtcccgc ggctcgttcg gtcgtccgag ccgctcgggc gacgcgagct cgccctcggg
     2101 gccgtgaccg ccgccggcat cgccgccttc tgcg
//
LOCUS       DAOPHV010000494         2132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_73406_length_2132_cov_4.526240, whole genome shotgun sequence.
ACCESSION   DAOPHV010000494 DAOPHV010000000
VERSION     DAOPHV010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73406_length_2132_cov_4.526240"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PLQ29_11275"
     CDS             <1..590
                     /locus_tag="PLQ29_11275"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPG87268.1"
                     /translation="AHDLAGLLDAIGLGRVVAVGTSYGAEVALTFARDYPGRCAGVAA
                     IDGVSEYDEVLRAAIECWRSAALADPRTFYKSLVPWTYSAEYLAARSDAIAAREDAVA
                     ALPRDYFEGFARLCDAFLAIDLTRDLGRVACPCLVVVGDRDILKPPRYSRIIADGVPG
                     ARYVEVAGAGHALAIEKPDELSGLLLGFLSEAAGP"
     gene            587..1480
                     /locus_tag="PLQ29_11280"
     CDS             587..1480
                     /locus_tag="PLQ29_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPG87269.1"
                     /translation="MTRFAGRAWIAVAFVAFVAFVALPAIVVVAACSSSRIMEETMVQ
                     KTVTVLGKSMYYVESGSGPAVVLVHGNTGSSRWWKKAMNLNGSRTIAPDLPNFGRSDA
                     LDTADIDLYADYLAAFIEALGLERPIVVGHSLGGAVVMAMAARRPELPGALVIVDGAA
                     PSGLVTPEAHYPYIELFRTNRDMMRQALAAVTPTMDDPAFLDELTEDAMAMAPHAFSG
                     NARALARFDYTGRAGAYTGPVMVVWGRKDSIITEAMARETAAAYRGSRLLVLDSVGHS
                     VMAEDPELFKRSLLDFIAGAD"
     gene            complement(1497..>2132)
                     /locus_tag="PLQ29_11285"
     CDS             complement(1497..>2132)
                     /locus_tag="PLQ29_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HPG87270.1"
                     /translation="DFDAVSESNLNRLYFGYSGEVGRLKTEVFADYAARVNPECHVEA
                     IGRIVHGAEAAGMIPADCGVYLDCIDTLNPKVNLMAALARAGLPFVSAMGTAGRLAPE
                     RLKVGRLWDATHCPLASDVRKRLRRLGFGPPGASLAALGDVGVSMPAAFECVWSDEPP
                     VPPAMPPDGSRPGDCPGGVRVRAVQGSGPFVPQAAGHLLASLAARRLLGRR"
BASE COUNT          305 a          747 c          756 g          324 t
ORIGIN      
        1 acgcgcacga cctcgcgggg ctactcgacg ccatcggcct gggccgcgtc gtggccgtgg
       61 gcacctccta cggggcggag gtcgcgctga ccttcgcccg cgactaccct ggacgatgcg
      121 cgggcgtcgc cgcgatagac ggcgtgagcg agtacgacga ggtgctccgc gccgccatcg
      181 aatgctggag atcggcggcg ctcgccgacc cccggacctt ctacaagtcc ctcgtgccct
      241 ggacctactc ggccgagtac ctggccgctc gcagcgacgc gatagccgcg cgcgaggacg
      301 cggtcgccgc cctgcccagg gactacttcg agggcttcgc gcggctgtgc gacgcgttcc
      361 tggccatcga cctgacccgc gacctcgggc gcgtcgcgtg cccctgcctc gtcgtggtcg
      421 gcgaccgtga tatactcaag ccgccgcgct actcgaggat catagccgac ggcgtgccgg
      481 gcgcccgcta tgtcgaggtt gccggggcag gccacgcgct ggccatcgag aagccggacg
      541 agctgtccgg cctgctgctc ggattcctgt ccgaggccgc cgggccgtga cgcgtttcgc
      601 gggacgagcc tggatcgccg tggcgttcgt cgcgttcgtg gcgttcgtag cgcttccggc
      661 gatagtcgtg gtcgcggcct gttcctcgtc ccggatcatg gaggagacta tggtgcagaa
      721 gacggtaacc gtactgggaa aaagcatgta ctacgtcgag tcgggctcgg gcccggccgt
      781 ggtgctcgtg cacggcaaca ccggctcgag ccgctggtgg aagaaggcga tgaacctgaa
      841 tggtagccgc accattgcgc cggacctgcc caatttcggc cggtcggacg cgctcgatac
      901 ggccgacatc gacctgtacg cggactacct ggcggccttc atcgaggcgc tcgggctcga
      961 gcggccgatc gtcgtcgggc acagcctcgg gggcgcggtg gtcatggcga tggcggcccg
     1021 ccggccggag ctgcccgggg cgctggtcat cgtcgacggg gcggcgccgt cgggcctcgt
     1081 cacgccggaa gcgcattacc cgtacatcga actgtttcgg accaatcgcg acatgatgcg
     1141 ccaggctctc gccgcggtaa ccccgacgat ggacgatccg gccttcctcg acgagctgac
     1201 ggaggacgcc atggcgatgg ccccacacgc gttctcgggt aacgcccgcg cgctggcgcg
     1261 cttcgactat accggccgcg caggcgctta taccggcccg gtcatggtcg tctggggccg
     1321 caaggattcg atcatcacgg aggctatggc ccgggagacc gccgcggcct accggggctc
     1381 caggctactg gtactcgact cggtcggcca ttcggtcatg gcggaggatc cggagctatt
     1441 caagcggagc ctcctcgact tcatcgcggg cgccgactaa cgccgactag cgccgactag
     1501 cgccttccga ggagccgccg cgccgccagg ctggcgagca ggtgcccggc cgcctggggt
     1561 acgaagggcc ccgagccctg taccgctcgc acgcgcacgc cgcccgggca gtcgccgggg
     1621 cggctaccgt cgggcggcat ggccggcggc accggcggct cgtctgacca tacgcactcg
     1681 aaggcggccg gcatcgatac cccgacgtcg ccaagggccg cgagcgaggc gcccggcggg
     1741 ccgaagccca ggcgccgcag ccgcttgcgc acgtcggagg cgagcggaca gtgggtcgcg
     1801 tcccagagcc gcccgacctt gagccgctcc ggcgccaggc gccccgccgt gcccatcgcc
     1861 gatacgaagg gcaagccggc gcgggccagg gcggccatca ggttgacctt ggggttgagc
     1921 gtgtcgatgc agtccagata gacgccgcag tcggccggga tcatgcccgc ggcctcggcg
     1981 ccgtgcacga tgcgtcctat cgcctcgacg tggcactccg ggttgactct cgccgcgtag
     2041 tcggcgaaga cctcggtctt gagccggccg acttcgccgg agtagccgaa gtacaggcgg
     2101 ttcaggttcg attcgctcac cgcgtcgaag tc
//
LOCUS       DAOPHV010000495         2129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_73546_length_2129_cov_4.516393, whole genome shotgun sequence.
ACCESSION   DAOPHV010000495 DAOPHV010000000
VERSION     DAOPHV010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73546_length_2129_cov_4.516393"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLQ29_11290"
     CDS             complement(<1..191)
                     /locus_tag="PLQ29_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPG87271.1"
                     /translation="MEGGKFHRKTFDRIDPERRAMILRVAMAEFASKGYSATGINELS
                     RKAGISIGSLYSYFASKEDL"
     gene            411..1415
                     /locus_tag="PLQ29_11295"
     CDS             411..1415
                     /locus_tag="PLQ29_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPG87272.1"
                     /translation="MKRAVVLSALLALVVSFAAAQQSVSAYTTLEEPLAKALFEKFEA
                     ETGIKVNFVRLSGGEVVTRLEAERANPQASIWVGGVGLDHITAKDKGLTTPYKSRAAG
                     KTPAEFKDKDGYWVGMYVGPLTFVTNTVRAAELGIKPPQSWADLLKPEYKGMIRVANP
                     NTSGTAYNVITTLRYIYKGDEQKVYDYLKKLDANIDQYTRSGSAPGKSVAIGEIPIAI
                     GYAHDQVKLKAGGAPVVITAPKEGTGYELASMSLVAGGKDAVNAKKLYDWVLSSPTAQ
                     AEFVNYYLVLVTPGAAKHPDALSLKEIKTVTQDFLWDGNADNRNRLLKRWTDEIGSKR
                     "
     gene            1488..>2129
                     /locus_tag="PLQ29_11300"
     CDS             1488..>2129
                     /locus_tag="PLQ29_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87273.1"
                     /translation="MPTKQRLIRYLAALAIFAAADIWIFATLRSTLRDDAARSRDSSL
                     ALLAKSAPTDLDLIGDWLASVPGSLPGGAAIYLEPSEDDPYAYEPYAADPAALALWLE
                     LEPSDDAQKGLDSAYYFENYASPAKATLDDTTVRLFYAPITDETEGELLGVAVFMAPE
                     TAAPGIERLLTVFAIGAFALFAALTGVSRFARDPTVGYAVLTLAGLSVVFIVFP"
BASE COUNT          405 a          746 c          630 g          348 t
ORIGIN      
        1 aggtcttcct tggacgcgaa atacgaatag agcgaaccga tcgagatgcc ggccttgcgg
       61 gacagctcgt tgatgcccgt cgccgagtag cccttggacg cgaactcggc catggccacg
      121 cgcagtatca tcgcccttcg ctcgggatcg atccggtcga aggtcttcct gtggaacttg
      181 ccgccttcca tcgcctctcc gttctacgct ctcgatgcgt cgcccagtcg accgcccgag
      241 caccgaatcc tacgccctgt cggctcgcag ggtcaagcgc ggcggctatg ccgaaaaatc
      301 caactagaat ccgctttctt caaccatatt taatttgcgc gccctaccaa gatgcgcgta
      361 caattataga aaatcaccgc gcgacagtaa tgtccgcgca cggaggaatc atgaagcgtg
      421 ctgtagtact atcagcgctc ctggcgctcg tcgtatcgtt cgccgcggcc caacaatcgg
      481 tcagcgccta tacgacgctc gaggagccgc tcgccaaggc cctgttcgag aaattcgagg
      541 ccgagaccgg gatcaaggtc aacttcgtac gcctgtccgg cggcgaggta gtcacccgcc
      601 tcgaagccga gcgggccaac ccccaggcct cgatctgggt cgggggcgtc ggcctcgatc
      661 acatcacggc caaggacaag gggctgacca cgccctacaa gtccagggcc gccggcaaga
      721 ccccggctga attcaaggac aaggacggct actgggtcgg catgtacgtg gggccgctca
      781 ccttcgtgac taataccgtc cgcgccgctg agctcggcat caagccgccg cagagctggg
      841 ccgacctcct gaagcccgag tacaagggca tgatacgcgt agccaacccg aacacctcgg
      901 gcacggccta taacgtgatc accacgctcc gctacatcta caagggcgac gagcagaagg
      961 tttacgatta cctcaagaag cttgacgcca acatcgacca gtacacccgc tccggctcgg
     1021 cccccggcaa gagcgtcgcg atcggcgaga tcccgatagc cataggctac gcacacgacc
     1081 aggtcaagct caaggccggc ggcgcgcccg tcgtgatcac agcccccaag gagggcaccg
     1141 gctacgagct ggcgtcgatg tccctggtcg ccggaggcaa ggacgcggtc aacgccaaga
     1201 agctctacga ctgggtccta tccagcccga ccgcccaggc cgagttcgtc aactattacc
     1261 tggtcctcgt gacgccaggc gccgccaagc acccggacgc cctgtcgctc aaggagatca
     1321 agaccgtgac ccaggacttc ctgtgggacg gcaacgccga taaccgcaac aggctcctca
     1381 agcgctggac cgacgagatc ggctccaagc gctagcgagc gcgcttccag aaagttaggg
     1441 gccggccgag aggtcggccc tattccgtat agtaaccgga gcgttccgtg cctaccaaac
     1501 agagactgat acgatacctg gcggccctgg cgatcttcgc cgccgcggat atctggatct
     1561 tcgcgacgct gcgctcgacc ctgcgcgatg acgccgcccg atcgcgcgac tcgtcgctcg
     1621 cactactcgc caagagcgcg ccgaccgatc tcgacctgat cggggactgg ctggcgtcag
     1681 tgccagggtc gctgccgggc ggcgcggcta tctacctcga gccgtccgag gacgacccgt
     1741 acgcgtacga gccctacgcc gccgaccccg ccgccctggc cctctggctc gagctcgagc
     1801 cgagcgacga cgcccagaag ggcctcgact cggcctatta tttcgagaac tacgcgtcgc
     1861 cggccaaggc gacgctcgac gatacgacgg tgcggctctt ctacgcgcca atcaccgacg
     1921 agacggaggg cgagctgctc ggcgtcgccg tcttcatggc gccggagacc gccgcgccag
     1981 gcatcgagcg cctgctgacc gtgttcgcga taggcgcctt cgccctgttc gccgcgctga
     2041 ccggcgtctc gcgcttcgcc cgcgacccga ccgtcggcta cgcggtgctc accctggcgg
     2101 gcctgtccgt cgtgttcatc gtcttcccg
//
LOCUS       DAOPHV010000496         2123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_73856_length_2123_cov_3.287234, whole genome shotgun sequence.
ACCESSION   DAOPHV010000496 DAOPHV010000000
VERSION     DAOPHV010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73856_length_2123_cov_3.287234"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2123)
                     /gene="rpoC"
                     /locus_tag="PLQ29_11305"
     CDS             complement(<1..>2123)
                     /gene="rpoC"
                     /locus_tag="PLQ29_11305"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPG87274.1"
                     /translation="AKSDKRLLKRIEIIESFRGSGNKPEWMIMDVIPVIPPELRPMVQ
                     LDGGRFATSDLNDLYRRVINRNNRLKRLQGLNAPDIIIRNEKRMLQEAVDALFDNSKK
                     KRVVKGASNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKIHQCGLPT
                     KMALELFKPFIMKKLVEKDVVYNIKKAKMLVEQETPEVFAVLDEVVREHPVMLNRAPT
                     LHRLGIQAFEPVLVEGKAIKLHPLVCRAFNADFDGDQMAVHVPLTHAAQSECWTLMLS
                     ARNLLDPANGKTIVYPSQDMVLGIYYLMRHKPDVKGQGHYFSSREEAYMACDAKACDW
                     EALIKAPVPKLPVWNSVGKDMADVTYGTTIETTAARLLFNEAMPPAVPFINYSMGEKE
                     LRALIEWVYKNEGPYVCVLMLDAIKDMGFKYATFFGATIGMDDIIIPKEKYDLINTAN
                     GQVEGIQKQYLAGHITQEERYNQVVEVWSKTNEDVTAVMMKTLEKDKKGFNNIHMMAH
                     SGARGSKNQIRQLAGMRGLMAKPGGDIIELPIRSNFKEGLSVIEFFISTNGARKGLAD
                     TALKTADAGYLTRRLVDIAQDVVINEDDCGTINGVAHASIKDGEEVVEHLRERIVGRY
                     TLERIKHPITGEIIIDVNEEITEEVAQRIEDAGIESVIIRTVLTCEARHGVCRKCYGR
                     NLATGRTVEIGEAVGIIAAQSIGQPG"
BASE COUNT          323 a          633 c          711 g          456 t
ORIGIN      
        1 cccggctggc cgatcgactg ggccgcgatg atgccgacgg cctcgccgat ctcgacggtg
       61 cgcccggtcg cgaggttgcg gccgtagcac ttgcggcaga cgccgtgcct ggcctcgcag
      121 gtgagcaccg tgcgtatgat caccgactcg ataccggcgt cctcgatacg ctgagcgacc
      181 tcctcggtga tctcctcgtt gacgtcgatg atgatctcgc cggtgatcgg gtgcttgatg
      241 cgctcgagcg tgtagcggcc gacgatgcgc tcgcggaggt gctcgacgac ctcctcgccg
      301 tcctttatgg aggcgtgggc cacgccgttg atggtaccgc agtcgtcctc gttgatgacg
      361 acgtcctggg caatatcgac gaggcgcctg gtcaggtaac cggcgtcggc ggtcttgagc
      421 gcggtatcag cgagtccctt tcgcgcgccg ttcgtcgaga tgaagaactc gatgacggac
      481 aggccttcct tgaagttcga ccgtatcggt agctcgatga tgtctccgcc cggcttggcc
      541 atgaggccac gcatgccggc gagctggcgt atctggttct tggagccgcg cgcgccggag
      601 tgggccatca tgtggatgtt gttgaagccc ttcttgtcct tctcgagggt cttcatcatc
      661 accgcggtca cgtcctcgtt ggtcttggac catacctcga cgacctggtt atagcgctct
      721 tcctgcgtga tgtgtccggc taggtactgc ttctggatgc cctcgacctg gccgttggcc
      781 gtgttgatca ggtcgtactt ttccttcggg atgatgatgt cgtccatgcc gatcgtcgcg
      841 ccgaagaacg tggcgtactt gaagcccatg tccttgatgg cgtcgagcat cagcacgcag
      901 acgtaggggc cctcgttctt gtagacccac tcgatcagcg cgcggagttc cttctcaccc
      961 atggagtagt tgataaaggg cacggccggg ggcatcgcct cgttgaacag cagccgcgcc
     1021 gcggtggtct cgatcgtcgt cccgtaggtg acgtcggcca tatccttgcc tacggaattc
     1081 cagacgggga gcttgggcac cggagccttg atcagggcct cccagtcgca ggccttcgcg
     1141 tcgcaggcca tgtaggcctc ttccctcgac gagaagtagt ggccctggcc cttgacgtcc
     1201 ggcttgtggc gcatgaggta gtagatgccg agcaccatgt cctgggacgg gtagacgatg
     1261 gtcttgccgt tcgcgggatc caggaggttc ctggcggaca gcatcagcgt ccagcactcg
     1321 gactgggcgg cgtgggtcag gggcacgtgg acagccatct ggtcgccgtc gaagtcagcg
     1381 ttgaaggcgc ggcagacgag cgggtgcagc ttgatcgcct tgccctcgac gagtaccggc
     1441 tcgaaggcct ggataccgag ccggtgcagg gtcggcgcgc ggttgagcat gaccgggtgc
     1501 tcgcgcacca cctcgtcgag caccgcgaag acctcggggg tctcctgctc gacgagcatc
     1561 ttggccttct tgatgttgta gacgacgtcc ttctcgacga gcttcttcat gatgaacggc
     1621 ttgaagagct cgagggccat cttggtcggc aagccgcact ggtggatctt gagctccggg
     1681 ccgacaacga tcacggagcg gcccgagtag tcgacgcgct tgccgagcaa gttctggcgg
     1741 aagcggccct gcttgccctt gagcatgtcg gacagggact tgagcggcct gttcgaggcg
     1801 cccttgacga cgcgcttctt cttcgagtta tcgaagaggg cgtcgacggc ctcctggagc
     1861 atgcgcttct cgttgcggat gatgatatcc ggggcgttga ggccctggag gcgcttgagg
     1921 cggttattac ggttgatgac gcggcggtac aggtcgttga ggtcgctcgt cgcgaagcgc
     1981 cccccgtcga gctggaccat cggcctgagc tccggcggga tgaccgggat cacgtccatg
     2041 atcatccact cgggcttgtt gccggagccg cggaagctct cgatgatctc gatgcgcttg
     2101 aggaggcgct tgtcgctctt ggc
//
LOCUS       DAOPHV010000497         2122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_73908_length_2122_cov_2.713595, whole genome shotgun sequence.
ACCESSION   DAOPHV010000497 DAOPHV010000000
VERSION     DAOPHV010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73908_length_2122_cov_2.713595"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLQ29_11310"
     CDS             complement(<1..383)
                     /locus_tag="PLQ29_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87275.1"
                     /translation="MRRRSRAIIAISASFSCLAALSSCDWISSLFPESFGPEYDDTSI
                     NYIASFPLEAPPAASDQGSVVALDAPGRWDWAWRGRTGNSFQYMTLSPAGTVGAAQTA
                     ASYTLAADEKVWRLELVNLAGDPYL"
     gene            complement(444..1598)
                     /locus_tag="PLQ29_11315"
     CDS             complement(444..1598)
                     /locus_tag="PLQ29_11315"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPG87276.1"
                     /translation="MPVPVYYLDARSRNARTSSVAKIRTLLAKLAEAAPDAVRKDDLC
                     AVKIHFGELGNDAYISPVFARAAADAALARGAKPFFTDTNTLYSGSRSNAIDHLNTAI
                     AHGFVPEVCGAPVVIADGLKGDDWREVPFPPEAAAASRLKSAKIAGGVLAADSMIVLS
                     HVKGHEMAGFGGAIKNLAMGCAPFIGKREQHCVKFQVNLERCVSCGQCASVCPTGAAT
                     LAKEKGSKATIDQEACIGCGECMTVCAPKAIGMDWDVGIEEFTEKMTEYALGAVLGKR
                     AAQGRPGRLWFVSVVQKVVPLCDCVPWNDAPVVPDIGFLASTDPVAIDQAAFDLVKAA
                     PVWPGSVLDGKAGAGDDKFTAMHPETRSELQMAHGERIGLGSRDYRLVRL"
     gene            1647..>2122
                     /locus_tag="PLQ29_11320"
     CDS             1647..>2122
                     /locus_tag="PLQ29_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87277.1"
                     /translation="MMDGRRGPWLRGVAAQVYADYRRDRVFVVGRLDDGRSFAAEDSS
                     WRPVVYLAAEDAASGESRFGGYRFETCELEAIDGRELVAWTAPGFGAYRDAVAALERA
                     GATVHGSGSIADLYRADLGLALGVRVSGDERPGRRVGLVLADAAVEADPDASAPLSV"
BASE COUNT          303 a          711 c          754 g          354 t
ORIGIN      
        1 tccaggtacg gatcccccgc cagattgacc agctcgagcc gccacacctt ctcgtcggcc
       61 gccagggtgt acgaagcggc cgtctgcgcg gcgcccacgg taccggcggg gctcagcgtc
      121 atgtactgga acgagttgcc ggtacggccg cgccaggccc agtcccagcg gcccggcgcg
      181 tcgagggcta cgacggagcc ctggtcgctc gcggccgggg gcgcctccag cggaaacgag
      241 gctatgtagt tgatgctggt gtcgtcgtac tcgggcccga acgactcggg gaagaggctc
      301 gatatccagt cgcacgacga cagcgcggcc aggcaggaga acgaggcgct tatcgctatg
      361 atcgctcggg aacggcgtcg catacccata ctatacctca ttatcggcgc cgcttcccgt
      421 tccgttcact cggggccgcg ggcttacagc ctgacgagcc ggtagtcgcg cgagccgagg
      481 cctatacgct cgccgtgggc catctgaagc tcgctgcggg tctcggggtg catcgcggtg
      541 aacttgtcgt cgccggcgcc ggccttgccg tcgaggacgg agccgggcca gacgggggcg
      601 gccttgacga ggtcgaaggc ggcctggtcg atcgcgaccg ggtcggtcga cgccaggaag
      661 ccgatgtccg gcacgacagg cgcgtcgttc cacgggacgc agtcgcagag cggcacgacc
      721 ttctggacga cgctgacgaa ccacagcctg cctgggcggc cctgagcggc ccgcttgccg
      781 agcacggcgc cgagggcgta ctcggtcatc ttctcggtga attcctcgat gcccacgtcc
      841 cagtccatgc ctatcgcctt gggcgcgcag acggtcatgc actcgccgca gcctatgcag
      901 gcttcctggt ctatcgtcgc cttcgagccc ttttccttgg cgagcgtcgc ggcgccggtc
      961 gggcagacgg aagcgcactg gccgcaggac acgcagcgtt ccaggttcac ctggaacttg
     1021 acgcagtgct gctcgcgctt gccgatgaac ggggcgcagc ccatcgcgag gttcttgatc
     1081 gcccctccga agccggccat ctcgtggccc ttcacgtggg acaagactat catgctgtcg
     1141 gcggcgagta cgccgccggc tatctttgcg gacttgagcc tggaggccgc ggcggcctcc
     1201 ggcgggaagg gaacctcgcg ccagtcgtcg cccttgaggc cgtcggctat gacgaccggg
     1261 gctccgcaga cctcgggcac aaagccgtgg gctatagcgg tgttcaggtg gtcgatcgcg
     1321 ttcgagcgcg agcccgagta cagggtgttc gtgtcggtga agaagggctt ggcgcctctg
     1381 gccagggcgg cgtcggcggc cgcccgcgcg aacacggggc tgatgtacgc gtcgttaccg
     1441 agctcgccga agtgtatctt gacggcgcat aggtcgtcct tacggacggc gtccggagcg
     1501 gcctcggcca gcttggctaa gagcgtccgg atcttggcca cggagctggt cctggcgttc
     1561 ctggagcggg cgtcgaggta gtatacgggt acgggcatgg cgtttccttc tggctctggt
     1621 atgtcagtcc atgatagcct aaacgcatga tggatggaag acggggaccc tggctcaggg
     1681 gcgtggcggc gcaggtctac gccgactacc gccgcgaccg cgtcttcgtc gtcggcaggc
     1741 tcgacgacgg gcggtccttc gccgcggagg actcgtcgtg gcggcccgtc gtctacctcg
     1801 ccgccgaaga cgccgcgtcc ggagaatccc gcttcggcgg gtaccgcttc gagacctgcg
     1861 agctcgaggc aatcgacggt cgcgagctgg tcgcgtggac cgctccgggc ttcggggcct
     1921 accgggacgc cgtcgcggcg ctagagcggg ccggcgcgac ggtccacggc tcaggctcga
     1981 tcgccgacct ataccgcgcc gacctcggcc tggccctcgg cgtccgcgtc tccggcgacg
     2041 agcggccggg ccgacgcgtc ggcctcgtgc tggccgacgc ggccgtagag gccgacccgg
     2101 acgcctccgc gccgctttcg gt
//
LOCUS       DAOPHV010000498         2120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_74002_length_2120_cov_3.476513, whole genome shotgun sequence.
ACCESSION   DAOPHV010000498 DAOPHV010000000
VERSION     DAOPHV010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74002_length_2120_cov_3.476513"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PLQ29_11325"
     CDS             <1..654
                     /locus_tag="PLQ29_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG87278.1"
                     /translation="KDYERAINSFQSYIGAFPEGDQVPASVFWSAECLYALGRLSDAE
                     KLYRSLGERYPDSVKAEAAKYRLSLIQFKYREDELLTLLKWSHEESLRIIEEFQRREK
                     AYEQALAVYQKRYGEAQRGVAQTQASLEDQVASLKLAMDDLAKRLQEKDARVVELEGE
                     LAGLSADDDRAAAEPSAAGGTVVASGEAELLAMKARALSLMQFYLERLAERSAGGSR"
     gene            651..1685
                     /locus_tag="PLQ29_11330"
     CDS             651..1685
                     /locus_tag="PLQ29_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87279.1"
                     /translation="MRRLTILALAAMAFAASAFGLEAKDGRIKLVVDDRTGRFSLYYL
                     VDVTRNRYEPLLYDQETRTTYATLMVDGKAFRLGDAPEFRIAVSREASGKVRVEYRSS
                     FCVVRQLFSFVTSPGAPMADGVTIQYSIENVSKAESTIGLRVLLDTYVGEKSAAHFVA
                     QGVGPLVSERALSGDYADSWVRGLGSAELSAANLQVLLGEPATRPDTLLAANWKRLND
                     SSWFFEANPSRNFTLLPYSINDSALALYYEPAPVRPGSTRAVVVGLSQAVEGYDAYVA
                     AKGDKPPVVAIAAPSATAPLDEMADLIAVRAVLEAIDAMLAAGQTPNAAELEALETTL
                     RRLEARKGQY"
     gene            1692..>2120
                     /locus_tag="PLQ29_11335"
     CDS             1692..>2120
                     /locus_tag="PLQ29_11335"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG87280.1"
                     /translation="MAKNPLDEAEALFRRRRWANLISLLEPLTAVYRESARFSVMLGC
                     AYLHREDTGGAYSCFRRAQSLDYRDVDAALGLAAVHIRRGESDKAVQQYIDALERHPR
                     DRRAKRGLGLLRKTASGGDEAVPSRQLRSLYPRPPRRWRLV"
BASE COUNT          366 a          711 c          695 g          348 t
ORIGIN      
        1 aaggactacg agcgggccat aaactcgttc cagtcctata tcggggcctt cccggagggc
       61 gaccaggtgc ccgcctcggt gttctggtcg gcggagtgcc tgtacgcgct cggacgcctc
      121 tcggacgccg agaaactata ccggtcctta ggcgagcgat accccgacag cgtcaaggcc
      181 gaggcggcca agtatcggct cagcctgatc cagttcaagt accgcgagga cgagctcctg
      241 actctgctca aatggagcca cgaggagtcc ctgcgcatca tcgaggaatt ccagcgccgc
      301 gagaaggcct acgagcaggc cctcgccgta taccagaagc gctacggaga ggcgcagcga
      361 ggcgtcgccc agactcaggc ctcgctcgag gaccaagtcg ccagcctcaa gctggccatg
      421 gacgacctgg ccaagcggct acaggagaag gacgcccgcg tcgtcgagct cgagggtgag
      481 ctggcgggtt tgagcgccga tgacgaccga gcggccgccg agccgtccgc tgcgggcgga
      541 acggtcgtcg cctccggcga ggccgagctt ctggcgatga aggcccgggc cctgagcctg
      601 atgcagttct acctggagcg cctcgccgag cgctccgccg gaggttctcg atgaggagat
      661 tgacgatact cgccctggcg gccatggcgt tcgccgcctc cgcgttcggt ctcgaggcca
      721 aagatgggcg gatcaagctc gttgtagacg atcggaccgg ccgcttctcg ctctattatc
      781 tcgtcgatgt cacgcgcaac cgctatgagc cgctcctgta cgaccaggag acccgtacca
      841 cctacgccac gctcatggtc gacggcaagg ccttcaggct cggcgacgcc ccggagttcc
      901 ggattgccgt gtcgcgggag gcctccggta aggtacgcgt cgagtaccgc tccagtttct
      961 gcgtcgtcag gcaactgttc tcgttcgtaa cgtcgccagg cgctcctatg gcagacgggg
     1021 tgacgatcca gtattccata gagaacgtct ccaaggccga gtcgacgata ggcctgcggg
     1081 tcctgctcga cacctacgtc ggcgagaagt cggccgcgca tttcgtggcg caaggagtcg
     1141 gtccgctcgt ctcggagcgg gcactgtccg gcgactacgc tgatagctgg gttcgcggcc
     1201 tcggatcggc cgagctttcg gccgccaacc tgcaggtgtt gctcggagag ccggctaccc
     1261 ggcccgatac cctcttggcc gccaactgga aacggctgaa cgactcgtca tggttcttcg
     1321 aggcgaaccc gtcgaggaac tttacgctac tgccgtactc gatcaacgac agcgccctgg
     1381 ccctgtacta cgagccggcc ccggtacgcc cgggttcgac tcgcgcggtg gtcgtcgggc
     1441 tgtcccaggc cgtcgaaggc tacgacgcct acgtggcggc caagggagac aagcccccag
     1501 tcgtcgcgat agcggctcct tccgcgaccg ctcccctcga cgagatggcc gacttgatag
     1561 ccgtgcgcgc cgtgctcgag gcgatcgacg ccatgctcgc ggctggccag accccgaacg
     1621 cggccgagct cgaggccttg gagaccacgc tcaggcggct cgaggcccgg aaggggcagt
     1681 actgataccc tatggcgaag aatccgctcg acgaggccga ggcgctgttc agacgccgtc
     1741 gctgggccaa cctcatcagc ctcctcgagc cgctgacagc ggtctatcgc gagtcggctc
     1801 gcttctccgt gatgctcggc tgcgcctacc tccacaggga ggatacgggc ggcgcctact
     1861 cgtgcttcag gcgggcacag tcgctcgatt atcgcgacgt cgacgccgcg ctcggcctcg
     1921 ccgcggtcca tatacgcagg ggcgagagcg ataaggcggt ccagcagtac atagacgcgc
     1981 tcgagcggca tccccgcgac cgtcgggcca agcgagggct cggcctactc cggaagacgg
     2041 cgagcggagg cgacgaagcg gtgccgtcca ggcagcttcg atccctgtac ccgagacctc
     2101 cgagacgctg gcgactcgtc
//
LOCUS       DAOPHV010000499         2116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_74192_length_2116_cov_3.776322, whole genome shotgun sequence.
ACCESSION   DAOPHV010000499 DAOPHV010000000
VERSION     DAOPHV010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74192_length_2116_cov_3.776322"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PLQ29_11340"
     CDS             complement(<1..777)
                     /locus_tag="PLQ29_11340"
                     /inference="COORDINATES: protein motif:HMM:NF025022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPG87281.1"
                     /translation="MADSSERKVFVVDTNILIHNPESVLSFRDNEIVIPLQVLEELDH
                     LKTYSDQRGKSARDAIRFLDGVSKRGDLHDGVKLENGSILRVSLAQSETAPKGFDTAK
                     NDNRIILCAAAIKESGRQVFFVSKDINARVKATALGVKAVDYEKEKVDIATLYQGVVE
                     VDVASDAIAALDATGNLPWKEAQRPNEFVLLRERGGAAERLARYRPDEGFVEALQGEL
                     GSVYGVSPLNARQRVALELLLDDRLSLVTIVGKAGTGKTLL"
     gene            complement(920..1477)
                     /locus_tag="PLQ29_11345"
     CDS             complement(920..1477)
                     /locus_tag="PLQ29_11345"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPG87282.1"
                     /translation="MRIRYNAPTTLTFTLICAIVLLFDSLLPGLTASWFSVPGRGGFD
                     PQSMRSYVRLFSHILGHADINHFLSNFSFILLLGPILESAYGSGGMAIMTLITAFVTG
                     ILNILLFQTGLMGASGIVFMMILLSSFTNFNKGEIPLTFILVLVLYLGKEVFNAFRDN
                     NISEFGHIIGGLCGSIFGYLRPARR"
     gene            complement(1502..>2116)
                     /locus_tag="PLQ29_11350"
     CDS             complement(1502..>2116)
                     /locus_tag="PLQ29_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPG87283.1"
                     /translation="RVYRPYWASAKRRGYSGVAVYSLKEPRSVRPLVIDEFDDEGRYL
                     EADFGELVVVSAYFPNSQEAGARLDYKLRFCDAVLARCDAIVAEGRHVSVAGDYNIAH
                     EPIDLTYPKANEGNPGYLPEERAWMTRFLSAGYADTFRRMHPGESGHYSWWSYRMKAR
                     EKDIGWRLDYHCVDEALFPAVRHAAIGKAVMGSDHCPVTLSLEL"
BASE COUNT          381 a          647 c          704 g          384 t
ORIGIN      
        1 gagcagcgtc ttgccggtgc cggccttgcc gacgatggta acgaggctga gcctatcatc
       61 gagcagcagc tcgagggcca cccgttgcct ggcgttgagg ggcgagacgc cgtagaccga
      121 gccgagctcc ccctgcagcg cctcgacgaa accctcgtcc ggcctatagc gggctaggcg
      181 ctctgccgcg ccgccgcgct cgcggagtag cacgaattcg ttgggacgct gcgcctcctt
      241 ccaaggcagg ttgccggtgg cgtcgagcgc ggctatcgcg tccgacgcca cgtcgacctc
      301 gacgacaccc tggtagagcg tggcgatgtc gaccttctcc ttctcgtagt cgaccgcctt
      361 gacgccgagg gccgtagcct tgaccctggc gttgatgtcc ttggacacga agaagacctg
      421 gcgcccggat tccttgatcg ccgcggcgca caggatgatc cggttgtcgt tcttggccgt
      481 atcgaagccc ttcggggccg tctccgactg ggcgagcgat acgcgcaaga tggagccatt
      541 ttcaagcttg acgccgtcgt ggagatcgcc ccgcttggag acgccgtcga gaaaacgtat
      601 cgcgtcgcgc gcgctcttgc cgcgctggtc cgaataggtc ttcaggtggt cgagttcctc
      661 gagcacctga agggggatga cgatctcgtt gtccctgaag gacagtaccg attccgggtt
      721 gtggatgagt atgttggtgt cgacgacgaa gaccttacgc tcgctggaat cagccatggt
      781 gccccctagt caggcgtttc gcgccatgcg tcaagtatag cgcgaggagc taggcgcgca
      841 aggccgcctg gccgccgtca gcgcctggcc gccgtcagcg cctggccgcc gttagcgcct
      901 ggccgccgcc tggccgccgt cagcgcctgg ccggccggag gtagccgaag atgctgccgc
      961 agagcccgcc tatgatgtgg ccgaattcgc tgatgttgtt gtcccggaag gcgttgaaca
     1021 cctccttgcc gaggtagagg acgaggacca ggatgaaggt gagcggtatc tcgcctttgt
     1081 tgaagttggt gaacgacgac aggagtatca tcatgaacac gatgccgctc gcgcccatca
     1141 ggccggtctg gaataggagg atgttcagga tgccggtcac gaaggcggtg atcagggtca
     1201 tgatggccat gccgcccgag ccgtaggccg actcgagtat gggcccgagc agcaggatga
     1261 acgagaaatt ggacaggaaa tggttgatat cggcgtgccc gagtatgtgg ctgaagagcc
     1321 gcacgtacga acgcatcgac tgcgggtcga agccgccgcg cccgggcacc gagaaccagg
     1381 aggccgtcag ccccggcaac aagctatcga acagcaggac gatggcgcat atcagggtga
     1441 aggtcagcgt ggttggcgcg ttatagcgta tcctcatatc gttctccagc gtatggcgga
     1501 atcaaagctc gagcgatagc gttaccgggc agtggtccga gcccatgacc gccttgccga
     1561 tggccgcatg gcggaccgcc gggaataggg cctcgtcgac gcagtggtag tcgaggcgcc
     1621 aacctatgtc cttctctcgg gccttcatcc ggtagctcca ccagctgtag tgaccggact
     1681 cgcccggatg catgcgcctg aaggtatcgg cgtagccggc cgacaggaag cgggtcatcc
     1741 aggcccgttc ctcgggcagg tagccgggat tgccctcgtt ggccttggga taggtcaggt
     1801 cgatgggctc gtgagcgata ttataatcgc ctgccaccga cacgtggcgc ccttcggcga
     1861 cgatagcgtc gcaacgcgcc aacacggcgt cgcagaagcg tagcttgtag tcgagcctgg
     1921 ccccggcctc ctgggaattc gggaaatagg cgctgacgac gacgagctcg ccgaaatccg
     1981 cctcgaggta gcgcccctcg tcgtcgaatt cgtctatcac caagggccgc acggatcgcg
     2041 gttccttaag cgagtacacg gcgacccccg aatagcctcg gcgcttggcg ctggcccagt
     2101 agggccggta cacgcg
//
LOCUS       DAOPHV010000500         2113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_74375_length_2113_cov_2.264820, whole genome shotgun sequence.
ACCESSION   DAOPHV010000500 DAOPHV010000000
VERSION     DAOPHV010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74375_length_2113_cov_2.264820"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1544
                     /locus_tag="PLQ29_11355"
     CDS             96..1544
                     /locus_tag="PLQ29_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87284.1"
                     /translation="MLILKLAFRNLMRHRRKTIVIGSLIAVGMAALFVANAVFESTNQ
                     GLRSSLIRSLTGDAALSARADTSFSLFGSEVPIVSEYESIPAMTEAGELLGLVNGLPE
                     TEASAPIVSALAQMRIGGIAVNVPVFGVEPDSYFKVCTDIAIERGDVSRLSDGGVFLN
                     GVLAASLEASLGRQLTLGEDVVFGMYSGGSFRLKKGSFAGVHRYVSSTETLDRVVLAD
                     PTLVRSIANYTLGYAMSSDDPAPTDDTFDLDSLFGDVSDSVAEGGSGLTLESVEDALA
                     DTSERDTLVMTDAAAWSFILVKARAGDDVRLARALFEAVRDREDEYRVLSWREAAGSS
                     AQALFAIQSAFYVGLGFVALGAVLVIMNALVISVLERSAEIGTMRGLGAGGPFIRGLF
                     VAESMALTLSAAAIGVVVGYIVALALAEGGIPLENPLLVSLFGGNVVRPAPSLRSAGM
                     HLVLAAFVGALAWIYPVTLAMRVQPVAAMSKA"
     gene            1549..>2113
                     /locus_tag="PLQ29_11360"
     CDS             1549..>2113
                     /locus_tag="PLQ29_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG87285.1"
                     /translation="MIAFLATRNLVLQRKRYALIALAVLLGFSLITVTSGAAYGALET
                     VKSKAARYFAGHVSVTGYYWGAPYMSNPDAVLETLEAADLPVRTVARRTIYYKTDAEL
                     FFGGETVRQRRLVGIDFAAERDELANLHFSDGSIDAMLGDDGRDGILISESAASLLGA
                     RVGDDVILYLTTDANQYNTATMIVRGVF"
BASE COUNT          336 a          653 c          703 g          421 t
ORIGIN      
        1 atgctgatac tgaaactagc catcaggttt ctgaaggcta gtttcagtat cagcatggtg
       61 ttcgggtgcc atttgctgta cagtacccga acaccatgct gatactgaaa ctagccttca
      121 ggaacctgat gcgccaccgg cgcaagacca tcgtcatagg tagcctcatc gccgtgggca
      181 tggccgcgct gttcgtggcc aacgcggtgt tcgagagcac caaccagggc ttgcgttcgt
      241 cgctcatacg gagcctgacg ggcgacgcgg ccctgtccgc acgggccgat acctccttca
      301 gcctgttcgg tagcgaggtg cccatcgtca gcgagtacga gtccatcccc gctatgacgg
      361 aggccggcga gctcctgggg ctcgtgaacg gtctgcctga gacggaggcg agcgcgccta
      421 tcgtgtcggc gctggctcag atgcgtatcg gcggtatagc ggtgaacgtg ccggtgttcg
      481 gcgttgagcc cgactcgtat tttaaggtct gtacggatat tgcgatagag cgcggcgatg
      541 tgtcgcggtt atccgacggc ggagtgttcc tgaacggcgt cctggctgcc tcgttggagg
      601 cctctctcgg ccggcagctg acccttggcg aggacgtggt tttcggcatg tactcgggcg
      661 gcagcttcag gctcaagaag ggtagtttcg ccggcgtcca tcgctatgtg agttcgactg
      721 agaccctgga tcgcgtggta ctggccgatc cgaccctggt acgttccatc gccaattata
      781 cgctcggtta cgccatgtcc tcggacgacc cggcgccgac cgacgatacc ttcgacctgg
      841 atagcctctt tggcgatgtt agcgacagcg tggccgaagg cggttccggg ctgacgctcg
      901 agtcggtgga agacgctctg gccgatacct ccgagcggga tacgctcgtg atgaccgatg
      961 ccgctgcctg gagttttata ttggtcaagg ctcgggctgg cgacgacgtt cgtctggcgc
     1021 gggcgctgtt cgaagccgtg cgagataggg aggacgagta tcgggtgctg agctggcgcg
     1081 aggccgccgg ctcgagcgcc caggccctgt tcgcgatcca gtccgcgttc tacgtgggcc
     1141 tgggcttcgt cgcgctcggc gcggtactgg tcatcatgaa cgccctggta atctcggtgc
     1201 ttgaacggag cgcggagata ggcacgatgc ggggactggg agccggcggt ccgttcatac
     1261 gcggcctgtt cgtcgccgag tccatggcgc ttaccctgtc ggccgccgcg atcggcgtcg
     1321 tggtaggcta tatcgtcgcc cttgccctgg ccgagggcgg catacccttg gagaaccccc
     1381 tgctcgtgtc gctgttcggc gggaacgtcg tgcgtccggc tcctagcctg cgtagcgcgg
     1441 gtatgcacct ggtgctggcg gccttcgtcg gcgccctggc ctggatctat cccgtgacgc
     1501 tcgccatgcg cgtgcagccg gtggccgcca tgagcaaggc gtagcgccat gatagcattc
     1561 ctggcgacgc gcaacctggt actgcagcgc aagcgctacg cgctcatagc cttggccgta
     1621 ctgctcggct ttagcctgat cacggtcaca tccggggcgg cctacggggc gctcgagacg
     1681 gtcaagtcca aggccgcgcg ttacttcgcg gggcacgtga gcgtgaccgg ctattattgg
     1741 ggcgcgccct acatgtcaaa cccggacgcc gtgctcgaga cgctcgaggc cgccgacctg
     1801 cccgttcgga ccgttgccag gcgaacgatc tactacaaga ccgacgccga gctgttcttc
     1861 ggcggcgaga cggtccgcca gcgcaggctc gtcggcatag acttcgccgc cgagcgggac
     1921 gagctagcca acctgcactt ctccgacggc tcgatagacg cgatgctcgg cgacgacggc
     1981 cgcgacggca tactgatcag cgagtcggcg gctagcctgc tcggcgcgcg ggtcggcgac
     2041 gacgtgatcc tgtacctgac caccgacgcg aaccagtaca ataccgctac catgatcgta
     2101 cgcggcgtgt tct
//
LOCUS       DAOPHV010000501         2111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_74442_length_2111_cov_3.863813, whole genome shotgun sequence.
ACCESSION   DAOPHV010000501 DAOPHV010000000
VERSION     DAOPHV010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74442_length_2111_cov_3.863813"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLQ29_11365"
     CDS             complement(<1..328)
                     /locus_tag="PLQ29_11365"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPG87286.1"
                     /translation="MESRSSVVTIAGRLKPARTATHSAPAAGYIAELFVRPGERVRAG
                     QALVSIGRDIPGESYRPLVVSSRIAGVVSTLALKEGEEVRSGAVLATVLDDSSFTLEA
                     AVSDKDA"
     gene            complement(650..>2111)
                     /locus_tag="PLQ29_11370"
     CDS             complement(650..>2111)
                     /locus_tag="PLQ29_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPG87287.1"
                     /translation="NSPEWAVCYLAVLFAGATVVPIDYQIKNEETVSIIKASDAVALF
                     VDEEKYDALDPAKLGLRARWSLSKKKPGYCYELAGASRTPDTAVESDLAAILFTSGTT
                     GTPKGVMLTHANLVSDCFLAQGNLSIFHTDVFYALLPIHHSYTMLAVFIEALSVGAEI
                     VFGQRMVTKAILKDLKEARVTMFLGVPLLFNKVLDGIMKGIKEKGALVYGLIRFLMSI
                     SGFVKKVFKVNPGKKMFGAILDKASLRTIRICISGGGPLAPSVFRMYNQLGIDFVQGY
                     GLTETSPIIALNPTEAYKEESVGKVLPGMEMRIIDKDDEGRGQIVVKGPMVMQGYYKN
                     DEATRESFRDGWLLTGDIGYLDDDDYLYLTGRAKNLIVTEGGKNVYPEEIENHFQLYT
                     EVEQVLVRGYLLDKKEKSEGIEACIYPNKDWFEQDGAKNGKVYDEAAIEAHIRKVVDE
                     VNADMLPYQRIGKVTMLAEPLEMTTTKKVKRFVAAN"
BASE COUNT          350 a          648 c          677 g          436 t
ORIGIN      
        1 acgcgtcctt gtcgctgacg gccgcctcga gcgtgaagga cgagtcgtcg agcaccgtcg
       61 cgagtacggc gccggagcgg acctcctcgc cttccttgag cgcaagcgtc gagacgacgc
      121 cggcgatgcg cgacgatacg acgagcggac gataagattc ccccgggatg tccctgccta
      181 tcgatacgag cgcctggcca gctcgaacgc gctctccggg cctgacgaac agctcggcga
      241 tatagccggc ggccggggcg ctatgtgtag ccgtcctggc gggcttgagc ctgccggcta
      301 tcgtgaccac ggagctacgg ctctccatcc gggccgtctt ggttctcggc ccgggcgtcg
      361 cgccttgaga tggcattccg ccgggcgaac ctcccgccgg cggtccgccc gatggctgac
      421 cgccggcgat agcggcggcg aggagcagta tgcatagtgt cgtcgcgtat cgtctcattg
      481 tatgtacctg taggcgctct tccgcgccgt atcccctgtg atgcatcgat ccgtgcatag
      541 tgtaaagcag gaggcggcgt cgtggataca aaaaacgcga gaccggccgg catgccgacg
      601 gtctcgcgtt catagctccg ttcgtcgagc tagttggcgg cgacgacgcc tagttggcgg
      661 cgacgaagcg ttttaccttc ttggtcgtcg tcatctcgag cggctcggcg agcatggtca
      721 ccttgccgat gcgctgatag ggcagcatgt cggcgttcac ctcgtccact accttacgga
      781 tatgggcctc gatggccgct tcgtcataga ccttgccgtt cttggccccg tcctgctcga
      841 accagtcctt gttcgggtag atgcaggcct cgatgccttc ggacttctcc ttcttgtcca
      901 ggagatagcc tcgtacgagc acctgttcca cctcggtgta gagctggaaa tggttctcga
      961 tctcctccgg atagacgttc ttgccgccct cggtaacgat caggttcttc gcgcgaccgg
     1021 tcaggtagag gtaatcgtcg tcgtcgaggt agccgatatc gccggtcaag agccagccgt
     1081 cacggaacga ctcgcgcgtc gcttcatcgt tcttgtagta gccctgcatg accatcgggc
     1141 ccttgacgac tatctggcct cgtccctcgt cgtccttgtc gatgatccgc atctccatgc
     1201 ccggaaggac cttgccgacg ctctcttcct tgtacgcctc ggtcgggttg agcgcgatga
     1261 tggggctggt ctcggtcagg ccgtagcctt ggacgaagtc gatgccgagc tggttgtaca
     1321 tcctgaacac gctcggcgcc agcgggccgc cgcccgagat gcagatgcgt atggtccgga
     1381 ggctggcctt gtcgaggatg gcgccgaaca tcttcttgcc cgggtttacc ttgaatacct
     1441 tcttgacgaa gccggagatg gacatcagga agcgtatcag gccgtagacg agcgctccct
     1501 tctccttgat gcccttcatg atgccgtcca gcaccttgtt gaacaggagc ggcacgccga
     1561 ggaacatcgt tacccgggct tccttcaggt ccttcaggat agccttcgtg accatgcgct
     1621 gaccgaacac gatctccgcg cctaccgaca gcgcctcgat gaagacggcg agcatcgtat
     1681 aggaatggtg gatgggcagg agggcgtaga agacgtccgt atggaagatg ctcaggttgc
     1741 cctgggcgag gaagcagtcg gagaccaggt tagcgtgggt gagcatgacg cccttcgggg
     1801 tgcccgtggt cccgctggtg aacaggatgg ccgcgaggtc ggactcgacc gccgtatctg
     1861 gcgtccgcga ggctccggcc agctcgtagc agtaacccgg ctttttcttg gacagagacc
     1921 agcgggcccg gaggccgagc ttggcggggt cgagcgcgtc gtacttctcc tcgtcgacga
     1981 acagggccac ggcgtcggac gccttgatga tcgagacggt ctcctcgttc ttgatctggt
     2041 agtcgatcgg tacgacggta gcgccggcga agaggacggc caggtagcag acggcccact
     2101 cgggcgagtt c
//
LOCUS       DAOPHV010000502         2097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_75202_length_2097_cov_3.523017, whole genome shotgun sequence.
ACCESSION   DAOPHV010000502 DAOPHV010000000
VERSION     DAOPHV010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75202_length_2097_cov_3.523017"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLQ29_11375"
     CDS             complement(<1..625)
                     /locus_tag="PLQ29_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPG87288.1"
                     /translation="MNLTTTRNIGIMAHIDAGKTTTTERILYYSGKTYKLGEVDDGEA
                     VMDWMEQEQERGITIQSAATTTFWRDHQINIIDTPGHVDFTAEVERSLRVLDGAVAVF
                     CAVAGVQPQSETVWRQAHRYHVPRIAFINKMDRVGADFDRAVATMRARLGANAVPLQM
                     PVGAEDRFNGVVDLVEFRMITWEGEGPTAEMVVSEVPEHLAGPARERR"
     gene            785..>2097
                     /locus_tag="PLQ29_11380"
     CDS             785..>2097
                     /locus_tag="PLQ29_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG87289.1"
                     /translation="MAKPKGERFERDLQYYKFCAYGFLKDLRFFDPFMILMFLDKGLS
                     YAQIGSLYALREVLINVMEIPSGVMADALGRRRTMIASFAAYLASFVVFYLARGYAVL
                     LLAMGVFAFGDAFRTGTHKAMIFDYLKLKGWADQKTHYYGHTRSWSQRGAALSSLIAA
                     ALVIWHGEYRVVFLFTLIPYALDLILMLSYPASLDGPRSAAYRAAIAGSGASAAAGPV
                     APAASEGADAHAASRSVAEEFRHVIKAFASSFKDLFVLKAISNQALYTGYYKAAKDYL
                     QPLLAGLALGLPVLLSWHEQERTALVVGLVYFVLYLATSYASRSSGRVSERFPSLARP
                     LNLTLLAGLGLGLASGLSYGLGLTALSALLYTGIYVIENLRKPMGIAYVSELMNQDAL
                     ATALSAESQAETLFAALVSLGLGFAADALGPGMGLAVVSGACLCLG"
BASE COUNT          300 a          741 c          670 g          386 t
ORIGIN      
        1 gccggcgctc gcgcgcgggc ccggccaggt gctccggaac ctccgagacg accatttccg
       61 cggtcgggcc ctcgccctcc cacgtgatca tccggaactc cacgaggtcc acgaccccgt
      121 tgaagcggtc ctcggccccg accggcatct gcagcggcac ggcgttcgcg ccgaggcgcg
      181 cccgcatggt cgcgaccgcg cggtcgaaat ccgcgccgac acggtccatc ttgttgatga
      241 aggcgatgcg ggggacgtgg tagcggtggg cctggcgcca gacggtctcg gactggggct
      301 gaaccccggc gacggcgcag aaaacggcca ccgcgccgtc gagcacgcgc aggctccgct
      361 ccacctcggc ggtgaagtcg acgtggccgg gcgtgtcgat gatgttgatc tgatggtcgc
      421 gccagaaggt cgtcgtcgcg gcgctctgga tcgtgatgcc gcgctcctgc tcctgctcca
      481 tccagtccat gacggcctcg ccgtcgtcca cctcgccgag cttgtaggtc ttgccggaat
      541 agtagaggat gcgctccgtc gtcgtcgtct tgcccgcgtc gatgtgcgcc atgatgccga
      601 tatttcgtgt cgtggtaagg ttcatgcggt gctccggccg ctactataga cggaaaagcg
      661 cttcgcttgc aatataagcg tacatttgcg agcataaccg agctcggcgc ggccggggta
      721 tcatcgacgc ccagccgggt accgtagccg cgaggttcgc ggccccagac gaggagtagc
      781 gaccatggcc aaaccgaagg gtgagcgctt cgagcgcgac ttgcaatact acaaattctg
      841 cgcgtacggc ttcctgaagg acctgcgctt cttcgatccg ttcatgatac tcatgttcct
      901 ggacaagggc ctgtcctacg cgcagatcgg cagcctctac gcgctccgcg aagtgctgat
      961 caacgtcatg gagataccct cgggcgtgat ggccgacgcg ctcggccgca ggcggacgat
     1021 gattgcgtcc ttcgccgcgt acctcgcgtc cttcgtcgtc ttctacctgg cccggggcta
     1081 tgcggtcctc ctgctggcga tgggcgtgtt cgccttcggc gacgcgttcc gcaccggcac
     1141 gcacaaggcg atgatcttcg attacctgaa gctcaagggc tgggcggacc agaagacgca
     1201 ctactacggg cacacgcgct cgtggagcca gaggggcgca gcgctatcgt cgctcatcgc
     1261 cgccgccctc gtgatctggc atggcgagta ccgcgtcgtc ttcctgttca cgctgatccc
     1321 gtacgcgctc gacctgatac tgatgctaag ctatccggcc agcctggacg ggccgcgtag
     1381 cgccgcctac cgcgccgcga tcgccggctc gggcgcctcg gcagcggccg gacccgtcgc
     1441 cccggcggcg tccgagggcg cagacgccca cgcggcgtcc cgctccgtgg ccgaggagtt
     1501 ccgccacgtg atcaaggcct tcgcgtcgag cttcaaggac ctcttcgtgc tgaaggccat
     1561 atcgaaccag gcgctctata ccggctacta caaggccgcc aaggactacc tccagcctct
     1621 cctcgccggc ctggcgctcg ggctgcccgt gctcctctcg tggcacgaac aggaacggac
     1681 cgcgctcgtg gtcggcctcg tctatttcgt tctctacctg gccacgtcgt acgcctcgcg
     1741 ttcgtcgggg cgcgtctccg agcgcttccc gtccctggcc cggcccctga atctcacgct
     1801 gctcgcgggg cttggcctcg gactcgccag tggcctgtcc tacggcctgg ggctgacggc
     1861 gctttccgcc ctgctctata cggggatcta cgtgatcgag aacctgagga agccgatggg
     1921 catcgcctac gtctccgagc tgatgaacca ggacgccctg gcgacggccc tgtcggccga
     1981 gtcccaggcg gagaccctct tcgccgcgct cgtctcgctc ggcctcggct tcgccgccga
     2041 cgccctcggc cccggcatgg gcctggccgt cgtctccggc gcctgcctct gcctggg
//
LOCUS       DAOPHV010000503         2095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_75291_length_2095_cov_3.521569, whole genome shotgun sequence.
ACCESSION   DAOPHV010000503 DAOPHV010000000
VERSION     DAOPHV010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75291_length_2095_cov_3.521569"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1942
                     /locus_tag="PLQ29_11385"
     CDS             <1..1942
                     /locus_tag="PLQ29_11385"
                     /inference="COORDINATES: protein motif:HMM:NF009881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG87290.1"
                     /translation="IRDAVESAKRERELYDRKTILFVDEVHRWNKSQQDALLPYVENG
                     TIVLIGATTEKPFFEVNKALVSRSRVFQLHPLGDDDLVAAARYALSDRERGYGRWKVE
                     FDDGALEHLVKVAEGDARSLLNALELAVETSVEPWPPADGERVLVTLDAAEQSIQRRA
                     VLYDRDGDYHYDASSALIKSLRGSDPDAALYWLARMVYAGEDPRFIFRRLLISAAEDV
                     GLADPAALGVVASCAEAYERIGLPEGQFLLAEAALYLATAPKSNSALAYFDALAAVEA
                     ERAEVPDHLKDANRDAEGLGHGAGYLYPHAYRSHWTAQQYLPDSLKGAVFYQPGSLGH
                     EARVRDEVLRRRDAQLAAWLSGELDVPRPTAAPTSHAQAASWTARADSSSAGGLSGLR
                     DLVFKLAFPPRHANVLVLAADDGLMLREAVRTCAEGSVCGVCRDAASIDRLAYAFKDV
                     EELLRPSLAACADAARVADTAMEAAGHAVFDLVVAAGFLSSYGAEAAVEGGRALVERL
                     RADGGRPAFVFCERSPVAGALPAIVAPLLEAGTLAAFTAADEAFFASSAAPGFSRERL
                     EEAIVAMGLEPRRSEPYRYDTQRSVGERDILAWLSADSAYGSAIASAIGAEVVAVVLD
                     ALKKGIESGQAAWPNLWNVTLA"
BASE COUNT          305 a          784 c          695 g          311 t
ORIGIN      
        1 catacgcgac gccgtcgagt ccgccaagcg cgagcgcgag ctgtacgacc gcaagaccat
       61 actcttcgtc gacgaggtgc accgctggaa caagtcccag caggatgccc tcctgcccta
      121 cgtcgagaac ggcaccatag tcctgatcgg cgcgaccacg gagaaaccct ttttcgaggt
      181 gaacaaagcc ctcgtgtcgc gctcgcgcgt gttccaactc catccgctcg gggacgacga
      241 cctggtcgcc gccgcgcgtt acgccctgtc cgaccgagag cgaggctacg gccgatggaa
      301 ggtcgagttc gacgatggag ccctcgagca cctggtcaag gtggccgaag gcgacgcgcg
      361 tagccttctc aacgcgctcg agctggccgt ggagacgagc gtcgagccct ggccaccggc
      421 cgacggggag cgggtcctcg tcacgctcga cgccgccgag cagagcatcc agcgacgcgc
      481 cgtactgtac gaccgcgacg gcgactacca ctacgacgcc tcgagcgccc tgatcaagag
      541 cctgcgcggc tccgaccccg acgcggccct gtactggctg gctcgcatgg tctacgccgg
      601 cgaggatccg cggttcatct tcaggcgact cctcatctcg gcggccgagg acgttggcct
      661 ggccgacccg gccgccctcg gcgtggtggc ttcctgcgcc gaggcctacg agcgcatcgg
      721 cctgcccgag gggcagttcc tcctggccga ggccgccctg tacctggcca ccgcgcccaa
      781 atccaactcg gcgctcgcct acttcgacgc gctcgcggcc gtcgaggcgg agcgagccga
      841 ggtgccggat cacctgaagg acgccaaccg cgatgccgag ggcctcggcc acggcgcagg
      901 ctacctctac ccgcacgcct accgcagcca ctggaccgcc cagcaatacc tacccgactc
      961 gctcaagggc gccgtcttct accagccggg ctcgctcggc cacgaggctc gcgtgcgcga
     1021 tgaggttctc aggcgccgtg acgcccagtt ggcggcctgg ctgtctggag agctcgacgt
     1081 cccgcggccg acggccgcgc cgacgagcca tgcgcaggcg gcttcctgga cggctcgcgc
     1141 cgactcgagc tcggccggcg ggctctccgg cctccgcgac ctcgtcttca agctggcgtt
     1201 cccgccgagg cacgcgaacg tgctcgtgct cgcggcggac gacggcctca tgttacgcga
     1261 ggcggtcagg acctgcgccg agggctctgt ctgcggcgtc tgccgcgacg ccgcttcgat
     1321 cgaccgcctg gcctacgcgt tcaaggacgt cgaagagctt cttcgcccga gcctcgccgc
     1381 ctgcgccgac gccgcgcgcg tggccgacac ggccatggag gcggcgggcc acgccgtctt
     1441 cgacctggtc gtcgccgcgg ggttcctgtc ctcctacgga gccgaggccg cggtggaggg
     1501 cgggcgggcc ctggtcgagc ggctacgcgc cgacggcggg cgcccggcct tcgtgttctg
     1561 cgagcgctcg ccggtagccg gagccttgcc ggccatcgtc gccccgctgc tcgaggcggg
     1621 aacgctcgcg gcgttcacgg ccgccgacga ggccttcttc gcgtcgagcg ccgcgccagg
     1681 cttctcccgg gagcgcctcg aggaggccat cgtcgccatg ggccttgaac cgagacggag
     1741 cgagccgtac cgctatgaca cgcagcgctc cgtcggcgag cgcgatatcc tggcctggct
     1801 atcggccgac tcggcctacg ggtcggccat agcctcggcc atcggcgccg aagtcgtcgc
     1861 cgtcgtactc gacgccctca agaaaggcat cgaatcaggc caggccgcct ggccgaacct
     1921 ctggaacgtg acgctggctt gagccggccg cgataggtcg ctatactccg cctccatggg
     1981 catcaggctc gtctacggac tcaagaccga cgacgcatcg tacctcgact ccttctaccc
     2041 cgcctggcgc ctccgcgagg ccgccgccgc caacggtctg gactatggcg cgacg
//
LOCUS       DAOPHV010000504         2080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_76053_length_2080_cov_3.481975, whole genome shotgun sequence.
ACCESSION   DAOPHV010000504 DAOPHV010000000
VERSION     DAOPHV010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76053_length_2080_cov_3.481975"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="PLQ29_11390"
     CDS             <1..1072
                     /locus_tag="PLQ29_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87291.1"
                     /translation="PATTRIPVPNPSGNWQLGVVDSLYSQSLEMYQVSGLSSDTHFAF
                     DKSGLLYASLPNGDVPGVGVFGTHLDTGPYDSIQFNPDYPVGSIALCSASNRIYSLCS
                     VEIEGTFNYYFQFVDLDDETLTPYDVTLDTPYAFDEYIDCGTLAVDALGYLYVPAYES
                     STETYGILRFSVGEPSGGTATATPLAFATLEELGLGYYDAESAFWPLAVEDMAIVGGT
                     LYIALSELNTQASLSGGILAGAAVCRGKIVALGIHPLQYLWEVGWRDDSDRRPESPDA
                     PGLFAPRRFLAVAPKRLYLLDEGFYVDVSKYSSGTWYPFEDVDRIAAVDVEARSLSFG
                     ARGLTDGLVNYDSDPYSTYANC"
     gene            1103..1981
                     /locus_tag="PLQ29_11395"
     CDS             1103..1981
                     /locus_tag="PLQ29_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87292.1"
                     /translation="MAGSGHVALGLSLGNALRRRARAGRAAEAEYRLVCVDSPFAPLA
                     SRLGFDIVTVPPEGEAELGPGAYEGSALYRAIVDYAPDLLVVDLFWFPLDSFIASLPC
                     RKVLLIRQVDPRFFTLPLRDRELRLRPGDYDLVLRTEPCFETPFPSEEIDPIIILDRG
                     EIMGRDEALADLGLAPDERACLFAFNGAEGEGALAWKSFSYLEDEGWRVVRSDNRSGG
                     LFPAARWFNAFDLVVCGAGYSAFWEARYFGKEACFVPYPRRFEDQARRIALCSDYTPA
                     NNGADELVDRLSALAE"
BASE COUNT          347 a          668 c          631 g          434 t
ORIGIN      
        1 tcccgcgacc acccgtattc ccgtgccgaa ccctagcgga aactggcagc tcggcgtcgt
       61 cgacagcctc tatagccaga gcctggaaat gtaccaggtc tctggcctga gcagcgatac
      121 tcatttcgcg ttcgacaagt cgggactgct gtacgcgtcg ctaccgaatg gcgatgttcc
      181 gggcgtcggc gttttcggga cccatcttga taccggtcca tatgatagta ttcaattcaa
      241 tccggattat cctgtaggaa gcatagcatt atgctcagct tccaatagaa tctacagcct
      301 ctgctctgtt gaaatcgaag gaacttttaa ctattacttc caatttgtcg atctcgacga
      361 tgaaactcta actccatatg atgtgacttt agatacacca tatgctttcg atgaatatat
      421 agattgtggt actcttgccg tagacgctct cggctacctc tacgtcccgg cgtacgagtc
      481 ctcgacggag acttatggca tcctccgctt ctcggtcggc gagccgagcg gcggcaccgc
      541 cacggctacg ccgctcgctt tcgccacgct cgaggagctc ggtctcgggt actacgatgc
      601 cgagtcagcc ttctggcccc tcgccgtaga agatatggcg attgtgggag gtacgctcta
      661 catcgccttg agcgagctga atactcaagc cagcctttcg ggcggaatac tcgccggcgc
      721 tgccgtctgc cgcggcaaga tcgtggcgct cggcatacat ccgctccagt atctctggga
      781 ggtcggttgg cgcgacgata gcgaccgtcg ccccgagtct ccggacgcgc ctggattatt
      841 cgcgccgagg cgtttcctcg ccgtcgctcc gaaacgcctg tacctactcg acgagggctt
      901 ctacgtcgac gtgagcaagt attcctcagg aacctggtac ccgttcgagg atgtcgacag
      961 gatagccgcc gtcgacgtcg aagcgcggtc cctgtcgttc ggcgctcgtg ggctgaccga
     1021 cggcctcgtc aactacgact ccgaccccta tagcacgtac gccaactgct agcccgtgcg
     1081 cgtcctcttc tgttctagcg gcgtggcggg gtcgggtcat gtcgcgctcg ggctatcgct
     1141 aggcaacgcg ctcaggcggc gcgctcgggc ggggcgcgcc gcggaagcgg agtatcggct
     1201 cgtgtgcgtc gactcgccgt tcgccccgct ggcctcaagg ctcggcttcg atatcgtcac
     1261 cgtgccgccg gagggcgagg ctgaactcgg ccccggcgcc tacgagggct cggccctcta
     1321 ccgggccatc gtcgactatg cgccggacct gctcgtcgtc gacctgttct ggttccccct
     1381 cgactcgttt atagcaagcc ttccgtgcag gaaggtcctg ctcatcaggc aggtcgatcc
     1441 gcgcttcttc acgctaccgc tccgggaccg cgagctgcgc ctgcggccgg gcgactacga
     1501 cctggtcctg cgcaccgagc cgtgcttcga gaccccgttc ccgtccgagg agatcgaccc
     1561 tatcatcatc ctcgaccggg gcgagatcat gggccgcgac gaggccttgg ccgacctcgg
     1621 cctggcgcct gacgagcggg cctgcctgtt cgcgttcaac ggcgcggagg gcgagggcgc
     1681 cctggcctgg aaaagcttct cgtacctgga ggacgagggc tggcgcgtcg tgcgttccga
     1741 taaccgctcg ggcgggctgt tcccagcggc ccgctggttc aacgccttcg acctcgtcgt
     1801 ctgcggcgcc ggctactcgg ccttctggga ggcgcgctac ttcggcaagg aggcctgttt
     1861 cgtgccgtat ccgaggcgct tcgaggacca ggcgagacgg atagcgctct gctcggacta
     1921 tacgccagcc aacaacggcg ccgacgagct ggtcgatagg ctatcggcgc ttgccgagtg
     1981 actacctacg attcggaacc ctagcgagcg gatgcgatgg tgggctatgt ggggtatact
     2041 ggtcgtatgg aaaccatcaa ggacaaggcc ctggcactgc
//
LOCUS       DAOPHV010000505         2079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_76104_length_2079_cov_3.784091, whole genome shotgun sequence.
ACCESSION   DAOPHV010000505 DAOPHV010000000
VERSION     DAOPHV010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76104_length_2079_cov_3.784091"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..708
                     /locus_tag="PLQ29_11400"
     CDS             61..708
                     /locus_tag="PLQ29_11400"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HPG87293.1"
                     /translation="MIIVLAGPSGSGKTSSCGIAVRLAREAGVRVAGMLCEAVFSGGS
                     KVGIDWRDLADEGAARRSLARRLPGFSQARSAGAPSQGRSPAFDDSNPHTVRFGSWEF
                     DRKALAEADAATAAAVSGGSGPIGAAPSLVIVDEIGPLELERGSGMVKTLMALDEAAS
                     PEWERAGDAERLGLTVVVARPDITDRLAARWPGSVRLDVSDVPFERTARRILEAL"
     gene            complement(744..1454)
                     /locus_tag="PLQ29_11405"
     CDS             complement(744..1454)
                     /locus_tag="PLQ29_11405"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HPG87294.1"
                     /translation="MRIRTAAALACLACAVAARDTFAFDVTSVPLHLYLSGLKEARPP
                     VVVDDYLVLSAQGAYRYVGAAFSYEGWRSVHSFEVNRYGVFVLAVPIPYGDAVTVRYR
                     LVLDGLWAADPANPAMERDGETGAAMSLATLPERPRTVLGVWDPAGDGAATFHFEGEP
                     GQRITVAGSFNGWDPFIHELEERSPGRYELRLSLPPGEHYYVFVYRGERIADPLNRRL
                     LYGKDGRPVSAITIAAAD"
     gene            1594..>2079
                     /locus_tag="PLQ29_11410"
     CDS             1594..>2079
                     /locus_tag="PLQ29_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87295.1"
                     /translation="MPTLQDIERFKASLKTLGKEPETLERWGETWHDLEAPPQGVPDD
                     IADLLQSDDAALGDAALGDAALGDAFSSFLDDIPLGDDQVAEPAPTDDFELPGGFGEE
                     SSPAGPPERETDDFTVPDSLLEDFGSLLETEEATEAAPTDDAPTEEPIEPIEPIESLE
                     AT"
BASE COUNT          352 a          690 c          716 g          321 t
ORIGIN      
        1 cggctttccg ctggcgtcgg tagatgtctc gttcgcaccg gccgaggact gaggcgagcc
       61 atgatcatcg tgctcgcggg gccgagcggc tcgggcaaga ccagctcctg cggcatcgcc
      121 gtacgattgg ccagagaagc cggggtccgg gtagcgggca tgctctgcga ggccgtcttc
      181 tcgggcggct cgaaggtcgg catcgactgg cgcgacctgg cggacgaggg ggcggcgcgg
      241 cgctcgctgg ccaggcgcct gccgggcttc tcgcaggctc ggtcggcggg cgctccgtcc
      301 caaggccgga gccccgcctt cgacgactcg aacccgcaca ccgtgcgatt cggctcctgg
      361 gaattcgata gaaaagccct cgccgaggcc gacgctgcga cggcggcggc cgtctccggg
      421 ggatccgggc cgataggcgc ggcgcccagc ctggtcatcg tcgacgagat agggccgctc
      481 gagctggaac gtggctccgg catggtaaag acgctcatgg ccctcgacga ggcggccagc
      541 ccagaatggg agcgagcggg cgacgctgag cgcctcgggc tcacggtcgt cgtcgccagg
      601 cccgacatca ccgacaggct agccgctcgc tggccaggct ccgtcaggct cgacgtctcg
      661 gacgtcccgt tcgaacggac agcgaggcgt atcctagagg cgctgtagtc gcttccggct
      721 tatttccgcc cagcgcccgg acgtcagtcc gcggccgcga tcgtgatggc tgagacgggt
      781 cggccgtcct tgccgtagag caggcgccgg ttgagcggat cggcgatgcg ttcgcctcgg
      841 tagacgaaga catagtagtg ctcgcccggc ggcaggctta gcctgagctc gtagcgcccc
      901 ggcgatcgct cctccagctc gtggatgaac ggatcccagc cgttgaacga cccggcgacc
      961 gtgatgcgct ggccgggctc gccctcgaaa tggaaggtcg ccgcgccgtc cccggccggg
     1021 tcccagacgc cgaggaccgt cctcggccgt tcggggagcg tggccagcga catggccgcg
     1081 cccgtctcgc cgtcgcgctc catcgccgga ttggccggat cggcggccca gagcccgtcg
     1141 aggaccagcc gatagcgcac ggtaaccgcg tcgccatagg ggatgggcac cgccagcacg
     1201 aagacgccgt agcgattcac ctcgaacgaa tggaccgagc gccagccctc gtacgagaag
     1261 gccgccccga catagcggta cgcgccctgg gcgctcagca cgagatagtc gtccaccacc
     1321 acaggaggcc tcgcctcttt gagccccgac aggtagaggt gcagcggcac ggacgtcacg
     1381 tcgaaggcga aagtgtctct agcggcgact gcgcaggcga ggcaggcgag cgccgcggct
     1441 gtacgaattc tcatcgatgc tccaataggg tgatgctcct atagtatcgg cccgcccagc
     1501 cgtccgcttg aggtcggtct gagggccgaa ggcggccaca gcgttttcgt cctatacatc
     1561 cttaattttt tgccgttatt ataagcatca gccatgccaa cactacagga tatagagcga
     1621 ttcaaagcct cactcaagac gctcggcaag gaaccggaga cgctggagcg atggggcgag
     1681 acctggcatg acctggaggc gccgccgcag ggcgtacccg acgatatagc ggacctattg
     1741 cagtccgacg acgcggcctt gggcgacgcg gccttgggcg acgcggcctt gggcgacgcg
     1801 ttctcctcgt tcctggacga tataccgctc ggcgacgacc aagtggcgga accggcgccg
     1861 accgatgatt tcgagttgcc cggcggcttc ggagaggagt cgtcccccgc tgggccgccc
     1921 gagcgggaga ccgatgactt cacggtaccg gactcgctgc tcgaggattt cggttcgttg
     1981 ctcgagacgg aagaggctac cgaagcggcc ccgacggacg acgctcctac ggaggaaccc
     2041 atcgagccga tagaacccat cgagtccctc gaggcgacc
//
LOCUS       DAOPHV010000506         2071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_76522_length_2071_cov_3.730159, whole genome shotgun sequence.
ACCESSION   DAOPHV010000506 DAOPHV010000000
VERSION     DAOPHV010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76522_length_2071_cov_3.730159"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PLQ29_11415"
     CDS             <1..780
                     /locus_tag="PLQ29_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HPG87296.1"
                     /translation="RAAGLNHFSCMLEASDRRSGRDLYPDVRAKAPAFFEREPGYSDM
                     LDSYRETGSLEESEKFEKSGSAKPSRYEWADRRIVKFMLENYGLLPITTDSHFGEYIS
                     WASDVVDHRGILDFFDVYKIMLSRAVERKIKLETTERVVPIIDGILGDEGFEESAVNV
                     RNDGAIADLPDWIAVEVPAVVGASGVNAIKVGELPKGYAALLRSYCGVYDLTAEAVIH
                     KRRDYAVQAILANPVVSKAAKVGELVDRMIAQQGRWLGYLR"
     gene            855..1907
                     /locus_tag="PLQ29_11420"
     CDS             855..1907
                     /locus_tag="PLQ29_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPG87297.1"
                     /translation="MATIKHIARLAGVSPTTVSNVLHGNTAKVSPAVRDRVLAIVAEE
                     NYAPNQGAVMLVRSRSRIIGVILFMEPRSGSTAIEDPFVSTIIGTLESEIRAAGYFIM
                     LRATSDKDEVLRLAATWKLAGLVMLCVPSEISSAIVRRIGSPVVFVDCYFADDGQSYH
                     SIRLDDEVGAYAMTNYLLSLGHRRFAFMANAADFPSVDSARYDGYRRAFAEAGLEAPA
                     DGLVTVPKDLDERLAMYRRLAAEPRPYSALFFSADFYAVEAIDYLSGLGVSVPGDLSI
                     VGFDDIWIARLSRPRLTTVHQDVVAKGKAASDMLMRLIHKEPVTDSDVRLPVRLEIRE
                     SAAESAGGPVSLPRER"
BASE COUNT          349 a          726 c          662 g          334 t
ORIGIN      
        1 agggccgccg ggctcaacca tttcagctgc atgctcgagg cgagcgaccg ccggagcggc
       61 agggatctgt acccggacgt gcgcgccaag gccccggcct tcttcgagcg cgagcccggc
      121 tactccgaca tgctcgactc gtaccgcgag acaggctcgc tcgaggagtc ggagaagttc
      181 gagaagtccg gctcagcgaa gccgagccgc tacgagtggg ccgaccggcg catcgtcaag
      241 ttcatgctcg agaactacgg gctcctgcct atcacgaccg acagccactt cggcgagtac
      301 atatcctggg cctcggacgt ggtcgaccac cgcggcatcc tcgacttctt cgacgtgtac
      361 aagatcatgc tgtccagggc cgtcgagcgg aagatcaagc tcgagacgac cgagcgtgtc
      421 gtgcccatca tcgacgggat actcggagac gagggcttcg aggaatcggc ggtcaacgtg
      481 cgcaacgacg gcgccatagc cgacctgccg gactggatag ccgtcgaggt gcccgccgtc
      541 gtcggcgcct caggcgtgaa cgctatcaag gtcggcgagc tgcccaaggg ctatgcggcc
      601 ctcctgcgct cgtactgcgg cgtctacgac ctgacggccg aggcggtcat ccataagcgc
      661 cgcgactacg cggtccaggc catcctcgcc aatcccgtgg tttccaaggc agcgaaggtc
      721 ggcgagctcg tcgaccggat gatagcccag cagggccgct ggcttggcta cttgcgctag
      781 aagcgcgttg tcgagcagta gacgcccctc gccgcggacg gatcctccgc ccgcagcgac
      841 gaggcaccga tagcatggct accatcaagc atatcgcccg cctcgcgggc gtcagcccca
      901 cgaccgtctc gaacgtgctg cacggcaata ccgccaaggt gtcgcccgcg gtgcgcgacc
      961 gcgtcctggc catcgtcgcg gaggagaact acgctccgaa ccagggcgcg gtcatgctcg
     1021 tgcgtagccg ctcgcgcatc atcggcgtca tcctgttcat ggaaccgcgc tccggtagca
     1081 cggccatcga ggaccccttc gtctcgacga tcataggcac cctcgagtcg gagatccggg
     1141 cggccggcta cttcatcatg ctgcgcgcaa cctcggacaa ggatgaggtc ctaaggctgg
     1201 cggccacctg gaagctggcc ggcctcgtca tgctctgcgt accgagcgag atatcgagcg
     1261 ccatcgtacg ccgcatcggc agcccggtcg tcttcgtcga ctgctacttc gccgacgacg
     1321 gccagtccta tcactcgata cggctcgacg acgaggtcgg cgcctatgcc atgacgaact
     1381 acctgctgtc gctcgggcat cgccgcttcg ccttcatggc caacgcggcc gatttcccga
     1441 gcgtcgacag cgcccgctac gacggctacc gtcgcgcctt cgccgaggct ggcctcgaag
     1501 cgccggccga cggcctcgtc accgtgccga aggacctcga cgagcgcctg gccatgtacc
     1561 gtcggctggc cgccgaaccg cgcccctata gcgccctctt cttttcggcg gacttctacg
     1621 ccgtcgaggc gatagactac ctgtccggcc tcggcgtctc cgtgccgggc gacctgtcca
     1681 tcgtcggctt cgacgatatc tggatcgccc gcctgtcgcg cccgcggctg accaccgtac
     1741 accaggacgt cgtggccaag ggcaaagccg cctccgacat gctgatgcgg ctcatccaca
     1801 aggaacccgt caccgactcg gacgtgcgcc tgcccgtgcg cctcgagata cgggagtcgg
     1861 ccgccgagtc cgccgggggc cccgtatcct tgccgcgcga gcgctgacgc tctagcgctc
     1921 cggggcgagc atggcgccgg gtatatcgcg acaacgcggg cagaggccct ctatgaagta
     1981 ttgtgacgac tcgaagaggt atccgtccgg cgcggagggc gcctcgggcg gcgtcagacg
     2041 agcgaggtcg tggacctcgc cgcaggagcg g
//
LOCUS       DAOPHV010000507         2071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_76529_length_2071_cov_3.139881, whole genome shotgun sequence.
ACCESSION   DAOPHV010000507 DAOPHV010000000
VERSION     DAOPHV010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76529_length_2071_cov_3.139881"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PLQ29_11425"
     CDS             complement(<1..593)
                     /locus_tag="PLQ29_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPG87298.1"
                     /translation="MTKDPYQVLGVSPGASDDDIAKAYRKLAKTYHPDVNHGSPEAAA
                     KMSEINAAYERIKSGAAAQGSSAYGQGQGGFGAQGTYGQAGQGGQGASDPFGFGFDPF
                     EFFSGGYAGGRQNASPLGQARAFINAGLFAKADDLLRSMDDRSAEWHCLRAVTQSGMG
                     NPIDALRHAKTAVQMEPGNPEYRGVLDQLQNGGQAYR"
     gene            complement(590..1441)
                     /locus_tag="PLQ29_11430"
     CDS             complement(590..1441)
                     /locus_tag="PLQ29_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HPG87299.1"
                     /translation="MFGFVSANPERLSPEAAERYQAGYCGLCHALGSRHGDAARLTVN
                     YDMTFLALYLAAVYDEPGERGTERCVVHPFKAHEYLGGRIADYAADMNVLLAYHDAMD
                     GWNDDRDALCLARAKLLEKEYRRVEAAYPRQSRAVGDRMAELAAIEKRGEPNPDLPAR
                     RFGELMGEIFVMDDEGDDERSGAVAAGLRDFGRALGAFVYIMDACVDLERDLRSESYN
                     PMVATPSDQFDDILDVLMAGCVRAHERLVALVPAMRDRDLIENILYSGVWTRYEAHKR
                     KKEKAAR"
     gene            complement(1507..2071)
                     /locus_tag="PLQ29_11435"
                     /pseudo
     CDS             complement(1507..2071)
                     /locus_tag="PLQ29_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
BASE COUNT          283 a          663 c          729 g          396 t
ORIGIN      
        1 tggcggtagg cctgcccgcc gttctggagc tggtcgagca cgccgcggta ctcgggattg
       61 cccggctcca tctgcacggc ggtcttggcg tgtctgagcg cgtcgatggg gttgcccatg
      121 cccgactgag tcacggcgcg gaggcaatgc cactcggcgc tccggtcgtc catcgacctg
      181 aggaggtcgt cggccttggc gaagagcccg gcgttgatga aggctcgcgc ctggccgagc
      241 ggggaggcgt tctggcggcc gcccgcgtag cctccggaga agaactcgaa ggggtcgaag
      301 ccgaagccaa aggggtcgga agcgccctgg ccgccctggc cggcttggcc gtacgttccc
      361 tgcgccccga agccgccttg gccctgaccg taggcgctcg aaccttgagc ggccgcgccg
      421 ctcttgatac gctcgtaagc ggcgttgatc tcgctcatct tggcggcggc ctcgggactg
      481 ccgtggttga cgtcggggtg gtaggtcttg gccagcttgc ggtaggcctt ggctatatcg
      541 tcgtccgagg cgcccggcga tacgccgagc acctggtagg gatccttggt catcgtgcgg
      601 ccttctcctt cttgcgctta tgggcctcgt agcgcgtcca gacgcccgaa tacagtatgt
      661 tctcgatcag gtcgcggtcg cgcatcgccg ggacgagcgc gacgagcctc tcgtgagcgc
      721 ggacgcagcc ggccatcagg acgtcgagta tatcgtcgaa ctggtccgag ggggtagcga
      781 ccatcgggtt gtacgactcg ctacgcaggt cgcgctcgag gtcgacgcag gcgtccatga
      841 tatagacgaa cgcgccgagc gcgcggccga agtcgcgcag gcccgcggct acggccccgg
      901 accgctcgtc gtccccctcg tcgtccatga cgaagatctc gcccatgagc tcgccgaagc
      961 gcctggccgg caggtcggga ttcggttcgc cgcgcttctc gatggccgcc agctcggcca
     1021 tgcgatcgcc gacggcgcgg ctctgcctcg gataggcggc ctcgacgcgg cggtactcct
     1081 tctcgagcag cttggcgcgg gctaggcata acgcgtcgcg gtcgtcgttc cagccgtcca
     1141 tagcgtcgtg gtaggccagg agcacgttca tgtcggcggc gtagtccgct atacggccgc
     1201 ccaggtactc gtgggccttg aacgggtgga cgacgcatcg ctccgttccg cgctcgcccg
     1261 gctcgtcgta gacggcggcc aggtataggg ccaggaaggt catgtcgtag ttgacggtca
     1321 gcctggcggc gtcgccgtgc ctggaaccga gcgcgtggca gaggccgcag tagccggcct
     1381 gatagcgctc ggcggcctcg ggcgacaggc gctcggggtt ggcgcttacg aatccgaaca
     1441 cgtgcgctcc tcgatggccg cgcgcagggc ctcgttggcg gccaggatct gggccgcgtc
     1501 cttcgacttg acggctttct tggcggcttt aatgagttcc tcgacggcgg ccttatcatc
     1561 gcgatcgagt cccttgagca tggaggaagc ctggcctatc aggcgctcgg cgtcgtcctt
     1621 ggccgatacc tcgctcttct gcctggcgtc ctcggcctgg tagcgcttgg cgtcgcggat
     1681 ggcgcgctcg atctcctcgt cgctaaggga ggagctcgcc tcgatggtga tggcctgggc
     1741 cttgccggtg ccgaggtctc gcgccgagac gttgacgatg ccgttggcgt cgatgtcgaa
     1801 gctcacctcg atctggggta cgccgcgcgg cgcccgccga atgccggaca gcttgaattt
     1861 gcctaaggtc ttgttgtgcc tggccatctc gcgctcgccc tggagcacgt ggacgtcgac
     1921 cgaggtctgg aagttcgccg ccgtggtgaa gacctggctc ttcttgatcg gtatcgaggt
     1981 gttgcggtcg atgatcctcg agaatacgcc gcccatcgtc tcgatgccca gggacagggg
     2041 cgtcacgtcg agaaggagga ggtccttgac c
//
LOCUS       DAOPHV010000508         2069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_76631_length_2069_cov_4.736346, whole genome shotgun sequence.
ACCESSION   DAOPHV010000508 DAOPHV010000000
VERSION     DAOPHV010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76631_length_2069_cov_4.736346"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PLQ29_11440"
     CDS             complement(<1..692)
                     /locus_tag="PLQ29_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPG87300.1"
                     /translation="MGRIIGIDLGTTNSCVSVMEGGEPLVIANSEGQRTTPSIVGFTG
                     KGERVVGQPAKNQMITNPENTVYSIKRFMGRRFEEVQNETSLVPYKIVAHQNDVRVDA
                     AGKLYSPQEVSAFILQKMKKTAEDYLGESVTEAVITVPAYFNDAQRQATKDAGRIAGL
                     EVKRIINEPTAASLAYGFNKDQKKEKTIAVYDLGGGTFDISILELGEGVFEVKSTNGD
                     THLGGDDFDRRV"
     gene            complement(703..1371)
                     /gene="grpE"
                     /locus_tag="PLQ29_11445"
     CDS             complement(703..1371)
                     /gene="grpE"
                     /locus_tag="PLQ29_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPG87301.1"
                     /translation="MSKHKHQDIPETQNEGQVTDQSRERTAGSPSQATPDSGADAVEA
                     RVKAAEAKAAAAEAKAVELEAEVSSLKDQYLRKLADYENFRKRMFREKEDAVQYANSQ
                     ILVDLVAVLDDFERAIKSSELSKDFASLHDGVGMIEKNLLSTLAGKYGLARFESVGTA
                     FDPNVHEAVMSEPGDCPEPTVVEEFIKGYKLRDRVLRSAKVKVRMPRPSGDSNEKSAP
                     EANA"
     gene            1648..>2069
                     /locus_tag="PLQ29_11450"
     CDS             1648..>2069
                     /locus_tag="PLQ29_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HPG87302.1"
                     /translation="MQFQRPSIIQEQRQKLSPQMIQSIRLMALPLQELKEEIQQEIEA
                     NPALEVLEDRSTMSLESIPEERESESEAVQRFENSSDPGYSRSGDDDSDAKRMFLEGA
                     ITYPETLQEHLLWQLRLQPITPSQREIGERLVQNLNADG"
BASE COUNT          358 a          649 c          618 g          444 t
ORIGIN      
        1 atgacgcggc ggtcgaagtc gtcgccgccg aggtgggtgt cgccgttggt gctcttgacc
       61 tcgaagacgc cctcgccgag ttcgagtatc gatatatcga aggtgccgcc gccgaggtcg
      121 tacacggcga tggtcttctc cttcttctgg tccttgttga agccgtacgc gagactggcc
      181 gcggtcggct cattgatgat gcgcttgacc tcgaggccgg cgatgcggcc cgcgtccttg
      241 gtggcctggc gctgggcgtc gttgaagtag gccggaaccg tgatcacggc ctcggtcacg
      301 ctctcaccca ggtagtcctc ggcggtcttc ttcatcttct gcaggatgaa ggcgctgacc
      361 tcctgcgggc tgtacagctt gcctgccgcg tcgacgcgga cgtcgttctg gtgagcgacg
      421 atcttgtacg gaaccaggct ggtctcgttc tgcacctcct cgaagcggcg ccccatgaag
      481 cgcttgatcg agtagacggt gttctccgga ttggtgatca tctggttctt ggccggctgg
      541 ccgacgacgc gctcgccctt gccggtgaag ccgactatgg acggggtggt tcgctgaccc
      601 tcggagttgg cgatgacgag gggctcgccg ccctccatca ccgacacgca gctattggtc
      661 gttccaagat cgattccgat tatccttccc atgggatgct ccttatgcgt tcgcctctgg
      721 cgctgatttc tcgttcgaat ccccgctcgg acggggcatg cgcaccttga ccttagcgct
      781 ccgcagtacg cggtcgcgca acttgtagcc ctttataaac tcctcgacca ccgtcggctc
      841 ggggcagtcg cccggctcgc tcatcacggc ctcgtgcaca ttcgggtcga aggccgtgcc
      901 gacggactcg aagcgggcca gcccgtactt gccggccagc gtcgatagca ggttcttctc
      961 tatcatgccg acgccgtcgt gcaacgacgc gaagtccttg gacagctcgg aactcttgat
     1021 ggcccgctcg aaatcgtcga gcacggccac gaggtcgacg aggatctggg agttggcgta
     1081 ctggaccgcg tcctcctttt ccctgaacat gcgcttgcgg aaattctcat aatccgccag
     1141 cttgcgcagg tactggtcct tgagagacga aacctcggcc tccagttcga cggccttggc
     1201 ctcggcggcc gcagccttgg cctcggcggc cttgaccctg gcctctacgg catcggcgcc
     1261 gctatcgggc gtcgcctggg aagggcttcc ggccgtccgc tcccgcgatt gatcggtaac
     1321 ctggccctcg ttctgcgttt ctggtatatc ctggtgcttg tgtttagaca tggcctcgct
     1381 ctacgaatat gataccccag ggtcttcgta cgcgcttgac gctgggagcc gtgcggctag
     1441 tatcatgtaa agtaataagg gtgattttaa agcgtcaagc cacttacggc attcttagac
     1501 gaataatcgt gaaaataggc acggttcttg taggttcggg ccaccgggct tacggtcgtt
     1561 cggcttgact ttagccgacg atgctatacc atttatctcg agggtagtcg ccccgcgtca
     1621 cgcatcgccg tcacgaaagg atcgtacttg cagttccaga gaccatcgat catccaggag
     1681 cagaggcaga agctcagtcc ccagatgatc cagtccatca ggctcatggc cctacccctc
     1741 caggagctga aggaagagat acaacaggag atcgaggcga atccagccct cgaagtgctc
     1801 gaggatcggt ccacgatgtc gctcgagtcc attcccgagg agcgcgaatc ggagtcggag
     1861 gccgtccagc gcttcgagaa cagctccgac ccgggctata gccgttccgg cgacgacgac
     1921 tcggacgcca aacgcatgtt cctcgagggg gcgatcacct accccgagac gcttcaggaa
     1981 cacctcctgt ggcagctcag gctccagcct atcaccccct cccagcgtga gataggcgaa
     2041 cgcctcgtcc agaacctgaa cgccgacgg
//
LOCUS       DAOPHV010000509         2066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_76776_length_2066_cov_4.473894, whole genome shotgun sequence.
ACCESSION   DAOPHV010000509 DAOPHV010000000
VERSION     DAOPHV010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76776_length_2066_cov_4.473894"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PLQ29_11455"
     CDS             complement(<1..381)
                     /locus_tag="PLQ29_11455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614150.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPG87303.1"
                     /translation="MRDEERRVLMSGREYVGAARIDGPLVFVRGTHPVGYRELVECVD
                     ADGNVRLGMVLDTSDDAVVVQVFEGTDGLTMPGTRTRFRGRPLTVGVSDRMLGRVFDG
                     LGRPVDGGPAPLADDEPDVNGRPIN"
     gene            complement(374..>2066)
                     /locus_tag="PLQ29_11460"
     CDS             complement(374..>2066)
                     /locus_tag="PLQ29_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPG87304.1"
                     /translation="MLELVKVGESGLIGEIIKLSGNGATIQVYEDTTGVAPLDNVYGT
                     GRPLSLELGPGLLGTIYDGIQRPLDALLALSGRFIERGVSAEALDRTRLWAFEPRVAV
                     GDEVSAGAVLGVVQETSRIEHRVCAPPGVSGRLVSVAPAGEYRVDETIAVVSGPDGER
                     ELSMMQRWPIREPRPVAQRKPLDIPLITGQRVIDTLFPLAKGGTVAIPGGFGTGKTMT
                     QHAIAKWCDADIIVYIGCGERGNEMTDVLNEFPKLVDPRTGKSLMERTILIANTSNMP
                     VSAREASVYTGVTLAEYYRDQGYHVAVMADSTSRWAEALRELSGRMEEMPAEEGFPAY
                     LPTRIAEFYERAGYAETLSGADGSVTIIGAVSPPGGDFSEPVTQHTKRFVRCFWGLDR
                     QLANARHYPAISWLDSYSEYLGDVRPWWEERVGSDWATAREEIMELLQKEVRLLQVVK
                     LVGPDALPDSQRFVIEVCSMFKNAFLQQSAFDDVDRYSTVEKQLRMLLAIMAYWRRGQ
                     EAIKRGVTLHKLKRVKAVQDLVKMKFTIPNESPEEFDRLMARLERGIDQLEAIYA"
BASE COUNT          321 a          733 c          670 g          342 t
ORIGIN      
        1 gttgataggg cggccgttga cgtccggctc gtcgtcggcg agcggcgccg gcccgccgtc
       61 gacggggcgg ccgaggccgt cgaagacgcg gccgagcatg cggtcggaca cgccgacggt
      121 cagcggcctg ccgcggaacc gcgtcctggt acccggcatc gtcaaaccgt cggtgccctc
      181 gaacacctgt acgacgacgg cgtcgtcgct cgtgtcgagc accatgccca gcctcacgtt
      241 cccgtcggcg tcgacgcact cgaccagctc gcggtagccg acgggatggg tgccccgcac
      301 gaacacgagc ggcccgtcta tcctggccgc tccgacgtac tcgcggccgg acatcaggac
      361 ccgcctctcc tcgtcacgca tagatagcct ccagctggtc gatgccgcgc tcaagcctgg
      421 ccatcaggcg gtcgaattcc tcgggcgact cgttcggtat ggtaaatttc atcttgacca
      481 ggtcctgtac ggcctttacc cgcttcagct tgtggagcgt cacgccgcgc ttgatggcct
      541 cctgccctcg gcgccaatac gccatgatgg ccagcagcat gcgtagctgc ttctccacgg
      601 tggagtagcg gtcgacgtcg tcgaaggcgc tctgctggag gaaagcgttc ttgaacatcg
      661 agcagacctc gatgacgaag cgctgactgt ccggcagggc gtccggaccg acgagcttga
      721 ccacctgcag gagccgcacc tctttttgga gcagctccat gatctcctcg cgcgcggtcg
      781 cccagtccga cccgacccgc tcctcccacc agggccgcac gtcgccgagg tattccgagt
      841 agctgtccag ccatgatatg gccggatagt ggcgcgcatt ggccagttgg cggtcgagcc
      901 cccagaagca gcgcacgaag cgcttggtgt gctgggtgac cggctcggag aagtcgccgc
      961 cgggcgggct gaccgcgccg atgatcgtca cggagccgtc ggccccggac aaggtctccg
     1021 cgtagccggc gcgctcatag aactcggcga tcctcgtcgg cagataggcc gggaagccct
     1081 cctcggccgg catttcctcc atgcgacccg acaattcgcg caacgcctcg gcccagcgcg
     1141 acgtcgagtc ggccatgacc gccacgtggt agccctggtc gcggtagtac tcggcgagcg
     1201 tcacgccggt gtacacgctc gcctcgcggg ccgacaccgg catgttcgag gtattggcga
     1261 tgagtatcgt gcgctccatg aggctcttgc cggtcctcgg gtcgacgagc ttcgggaact
     1321 cgttgagcac gtcggtcatc tcgttgccgc gctcgccgca gccgatgtac acgatgatgt
     1381 cggcgtcgca ccacttggct atcgcgtgct gggtcatcgt cttgccggtg ccgaagccgc
     1441 cgggaatggc cacggtgccg cccttggcca gcgggaacag cgtgtcgatc acgcgctgcc
     1501 cggtgatcag cggtatgtcg agcggcttgc gctgcgcgac cggccgaggc tcgcgaatcg
     1561 gccagcgctg catcatcgac agctcgcgct cgccatccgg ccccgagacg accgcgatcg
     1621 tctcgtcgac ccggtactcc ccggccggcg ccaccgatac gagccgcccg gaaacgccgg
     1681 gaggagcgca gacccggtgc tctatcctcg aagtctcctg caccacgccg agcaccgcgc
     1741 ccgctgacac ctcgtcgccg accgctacgc gcggctcgaa ggcccagagc ctggtacggt
     1801 cgagcgcctc tgccgagacg ccacgctcga tgaagcggcc cgacagggcc aggagcgcgt
     1861 caagcggccg ctggatgcca tcgtagatgg tcccgagcag gcccggaccg agctcaagcg
     1921 atagcggccg gccggtgccg tacacgttgt cgagcggcgc cacgcccgtg gtgtcctcgt
     1981 agacctggat cgtcgccccg ttcccggaca gcttgatgat ctcgcctatc aggcccgact
     2041 cgccgacctt gaccagctcg agcatg
//
LOCUS       DAOPHV010000510         2064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_76884_length_2064_cov_4.792932, whole genome shotgun sequence.
ACCESSION   DAOPHV010000510 DAOPHV010000000
VERSION     DAOPHV010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76884_length_2064_cov_4.792932"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLQ29_11465"
     CDS             <1..275
                     /locus_tag="PLQ29_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87305.1"
                     /translation="VETLSCASCEREWVVPRPVPAAPRPKLRGEPPDDSPAGSCPSCG
                     KVFCVACRKDGLVDGRFTCPDCGERLNLNDDRVRWIVLERLKGLPE"
     gene            369..1040
                     /locus_tag="PLQ29_11470"
     CDS             369..1040
                     /locus_tag="PLQ29_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HPG87306.1"
                     /translation="MKALPSIVLAALLAASPAMAQDILTASDFFKGVSERYASIKDYE
                     AAVEVKAGDQLMAGTVIYKAPSLMRMDFSKPASQVIVYDGQQLTIYVPELRAVLTQQT
                     SSSASAAAVTGEGLRMLGRNYSVTYETGPTPTPLPGAEGENVIRLVLSRLTVAEGFRT
                     ITLSIAPETRLIRRLEGVTLAGDLISYDFKAIKLDQDIPGTRFIYDSPASANMYNNFL
                     FSSDN"
     gene            1058..>2064
                     /locus_tag="PLQ29_11475"
     CDS             1058..>2064
                     /locus_tag="PLQ29_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPG87307.1"
                     /translation="MNAIAIGEQLRTAREARGLTIDYVADETNIAKRYIAAMEEENFS
                     VFPGEPYIIGFLRNYAEYLGLESASIVQAFRGIRIQEQPAPIQELLQPKRARIWPFVA
                     VGLAAALGVATFLILRPGSDDVATDGPAMTRGELVQHRFDAAVLEKRLYPGDTLLVPY
                     GGDVYRIVLTGIDERVAIETPVGVSRFMLGEEGAIDLDKDNQAELYAFIVDFQKNDQS
                     RGAMIRFKAAAPLAEELPTAADQADAGAGAGVAGTQPATAPVVVALPTGAERPSTQES
                     IIFSGKRSPHPFVVNVTFRNYAMFRHEIDRKDREERYYHKGDQISVTANNSAKLWTSN
                     AA"
BASE COUNT          387 a          684 c          627 g          366 t
ORIGIN      
        1 ccgtggagac cctgagctgc gcttcctgcg aacgcgagtg ggtcgttccg aggcccgttc
       61 cggccgcgcc tcgccccaag ctacgcggcg agccccctga cgactcgccg gccggatcct
      121 gcccttcctg cggcaaggtc ttttgcgtgg cctgtcgcaa agacgggctc gtggacggca
      181 ggttcacctg ccccgactgc ggagagcgtc tcaacctgaa cgacgacagg gttaggtgga
      241 tcgtgctcga gcggctcaag ggactccccg agtgacgata ctcggcccgg acggcctcat
      301 cgcttgccat tttaccggct agcggatact atatcgaaga atccacgacg cacggtaggt
      361 tattcgacgt gaaagctctc ccttcgatcg ttctagcggc cctcctcgcc gcctcgccgg
      421 cgatggccca ggatattctc accgccagcg acttctttaa aggagtctcc gaacgctacg
      481 cctcgatcaa ggactacgag gcggccgtcg aggtaaaggc cggcgaccag ctgatggccg
      541 gcacggtgat ctataaggca ccgtcgctga tgcgcatgga tttctccaag ccggcctcgc
      601 aggttatcgt ctacgacggc cagcagctga ccatctacgt tcccgaacta cgcgccgtgc
      661 tcacccagca gacgagctcg tccgcctccg cggcggccgt caccggcgag ggcttgcgca
      721 tgctcggcag gaactattcc gtgacctacg agaccgggcc taccccgacg ccgcttccgg
      781 gtgcggaagg ggagaacgtg atccggctcg tactgtcgag gctcacggtg gccgagggct
      841 tcaggaccat cacgttgtcc atagcgccgg agacgcggct gatacgcagg ctggagggcg
      901 tgactctggc cggcgacctc atctcgtacg actttaaggc tatcaagctc gatcaggata
      961 taccgggaac gcggttcatc tacgattcgc cggcgtccgc caatatgtat aacaatttct
     1021 tattcagttc cgataactag ggagcgacga cgtatccatg aacgctatcg cgattggtga
     1081 acagctacga acggctcgcg aggcacgcgg ccttaccatc gactatgtcg ctgacgagac
     1141 gaacatcgct aagcgctaca tagccgctat ggaggaagag aacttctccg ttttcccagg
     1201 cgaaccgtat ataatcggtt ttctcaggaa ctacgccgag tacctcggcc tggagtcggc
     1261 ctctatagtg caagcgttca ggggtattcg catccaggag cagccggcgc ccatccagga
     1321 gctcctccag ccgaagcgcg cgcggatctg gcccttcgtc gccgtgggct tagcggcggc
     1381 gctcggcgtg gccacgttcc tgatcctgag gccgggctcc gacgacgtgg cgaccgacgg
     1441 gcccgcgatg acgaggggcg agctggtcca gcaccggttc gacgcggccg tcctcgagaa
     1501 acgtctgtac ccgggcgata ccctgctggt gccctacgga ggcgatgtct atcgaatcgt
     1561 ccttacgggc atcgacgagc gcgtggccat cgagacgccg gtcggcgtct cgcgcttcat
     1621 gctcggcgag gaaggcgcga tcgacctcga caaggacaac caggccgaac tgtatgcctt
     1681 catcgtcgac ttccagaaga acgaccagtc tcgcggcgcg atgatacgct tcaaggccgc
     1741 cgccccgctc gccgaggagc tgccgaccgc cgccgaccag gccgacgccg gtgccggtgc
     1801 cggcgtggcc ggaacgcagc ccgctacggc tcccgttgtc gtcgcgttgc cgaccggggc
     1861 cgagaggcct tcgactcagg aatcgatcat cttctcgggc aagcgcagcc cccatccctt
     1921 cgtcgtcaac gtgaccttca ggaactacgc catgttccgc cacgagatcg accgcaagga
     1981 ccgggaggag cgctactacc ataagggcga ccagatcagc gtcaccgcca acaactccgc
     2041 caagctatgg accagcaacg ccgc
//
LOCUS       DAOPHV010000511         2059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_77141_length_2059_cov_4.920160, whole genome shotgun sequence.
ACCESSION   DAOPHV010000511 DAOPHV010000000
VERSION     DAOPHV010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77141_length_2059_cov_4.920160"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PLQ29_11480"
     CDS             <1..699
                     /locus_tag="PLQ29_11480"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HPG87308.1"
                     /translation="AHPEPRSVLVLGGGDGGALREALRHRTVETVDFVELDPDVVAFS
                     REHLTHVHRGSFDDPRVRFHYRDGRAFVEAAPPAYDVVIMDMTDPAGPARFLYTKEFF
                     AAVRRSLRDERGVFSMHGESPAARPAAYACIGATLRSVFPVVSAAAAFVPMYGTLWSF
                     RYASATVDPSVPGRDELERRVAERMDGRPSFATPAMWSSLFSPDPMLAEAEADPLGRI
                     VTDAEPDFPDAFGL"
     gene            727..1293
                     /locus_tag="PLQ29_11485"
     CDS             727..1293
                     /locus_tag="PLQ29_11485"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPG87309.1"
                     /translation="MASGDEAWLETGRVRLADYGIVTVDRARRRGPHGKEGSFVILDA
                     PDWAVVVPALERDGRRLLLTVRQYRHGSRSVSDEFPGGVIEPGEAPGAAAARELLEET
                     GYQAARLVPIGSCSPNPAFMANTFHVFAAEGLELVSAQSLDEHELVDVEAVPEDELLA
                     RVGVAPWDHALMIAAAFFYERYRAGSVS"
     gene            complement(1290..1832)
                     /locus_tag="PLQ29_11490"
     CDS             complement(1290..1832)
                     /locus_tag="PLQ29_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87310.1"
                     /translation="MTTVSEWPQLRRLAAAVEIDWNGWLGSGASVGTDDHSLSFERDD
                     ETSTLDRSGVSARATPLRVDEGILAALGVAAPRWLTAKAGVAVDFELARQRRYGYDPP
                     SDPIRFFVESSETLSWRPVHFNELGLIVGPASLSTGFSSTIAWGAARAESTTAGSLVI
                     PRSARHSTNGEIPRPEPSSS"
     gene            complement(1829..>2059)
                     /locus_tag="PLQ29_11495"
     CDS             complement(1829..>2059)
                     /locus_tag="PLQ29_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87311.1"
                     /translation="RIFLVDQYYETAYSTSVAATPPATSYSWTGLSSGSKYAFVIVPY
                     DDTSTPKRIAARLFTGTDRWTVDSSSLFVTVP"
BASE COUNT          294 a          733 c          700 g          332 t
ORIGIN      
        1 gcgcatccgg agccccgttc cgtgctcgtt ctcggcggcg gcgacggcgg ggccctgcgc
       61 gaggccctgc gccataggac cgtcgagacg gtcgacttcg tggaactcga cccggacgtg
      121 gtcgccttca gccgcgagca cctgacgcac gtgcaccgcg gatccttcga cgacccgcgc
      181 gtgcgtttcc attaccgcga cggccgggcc ttcgtcgagg ccgcgccgcc cgcctacgac
      241 gtcgtcatca tggacatgac cgaccccgcc ggaccggccc gcttcctcta cacgaaagaa
      301 ttcttcgccg ccgtccggcg ttcccttcgg gacgagcggg gcgtcttctc gatgcacggc
      361 gagtcgcccg ccgccaggcc ggccgcctac gcgtgcatcg gcgctacgct acgctccgta
      421 ttcccggtcg tctcggccgc ggccgcgttc gtgccgatgt acggcacgct gtggtcgttc
      481 cgctacgcct cggcgaccgt cgacccgtcg gtcccgggcc gggacgagct ggagcgccgc
      541 gtagccgagc gtatggacgg tcgcccgtcg ttcgcgacgc cggcgatgtg gtcctcccta
      601 ttctcccccg acccgatgct cgccgaggcc gaggccgacc cgctaggcag aattgtcacc
      661 gacgcggagc ctgacttccc ggacgcgttc gggttatagt cgctcgatag cgacgaggag
      721 gcggcgatgg cgagcgggga cgaggcgtgg cttgagacgg ggcgggttcg gctggccgat
      781 tacggtatcg ttacggttga tcgcgcgagg cggcgcgggc cgcacggcaa ggaaggctcc
      841 ttcgtcatcc tcgacgcacc cgactgggcc gtcgtcgtcc cggccctcga gcgcgacggc
      901 cgacgactcc tgctgaccgt ccgccagtat cggcacggct cgcgttccgt ctccgacgag
      961 ttccccggcg gcgtcatcga gccaggcgag gctcccgggg ccgccgccgc gcgcgagttg
     1021 ctcgaggaga ccggctacca ggccgccaga ctcgtgccca tcggctcgtg ctcgccgaac
     1081 ccggcgttca tggccaatac cttccatgtc ttcgccgccg agggcctcga gctcgtctcc
     1141 gcccagtcgc tcgacgagca cgagctcgtc gacgtcgagg ccgtgcccga ggacgagctc
     1201 ctggcccgcg tcggggtcgc cccctgggac cacgccctga tgatcgccgc ggccttcttc
     1261 tacgagcgct accgggctgg ctcagtatcc tagctcgagc tcggctccgg cctcggtatc
     1321 tccccgtttg tagagtgacg agcggacctg ggaatcacca gcgagccggc cgtcgtcgat
     1381 tcggcgcggg cggcgcccca ggctatcgtc gagctgaagc cggtggacag cgaggcaggg
     1441 ccgacgatca agccgagctc gttgaagtgc accggacgcc atgacagcgt ctcggacgac
     1501 tcgacgaaga accggatagg gtcggagggc ggatcgtagc cgtagcgccg ctgccgcgcc
     1561 agctcgaagt ccacggcgac gccggctttc gcggtcagcc atcgcggcgc ggccacgccg
     1621 agagccgcga ggattccctc gtcgactcga aggggcgttg cccgcgcgct gacgcccgag
     1681 cggtcgaggg tcgatgtctc gtcgtcgcgc tcgaaggaca gcgagtggtc gtcggtgccg
     1741 accgacgcgc ccgagccgag ccatccgttc cagtcgatct cgaccgcggc ggcgagcctg
     1801 cgtagctgcg gccattcgga tacggtcgtc acggcacggt cacgaacaaa gacgagctat
     1861 ccaccgtcca tcggtccgtc ccggtgaata gccgggccgc gatccgcttc ggcgtcgagg
     1921 tatcgtcata tggcacgatg acgaacgcgt acttcgagcc ggaactcagg cccgtccagc
     1981 tgtagctcgt cgcaggcggc gtcgccgcga cgctcgtgga gtaggccgtt tcgtaatact
     2041 ggtcgacgag gaaaatcct
//
LOCUS       DAOPHV010000512         2058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_77206_length_2058_cov_3.907639, whole genome shotgun sequence.
ACCESSION   DAOPHV010000512 DAOPHV010000000
VERSION     DAOPHV010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77206_length_2058_cov_3.907639"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PLQ29_11500"
     CDS             <1..552
                     /locus_tag="PLQ29_11500"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPG87312.1"
                     /translation="PKGSDLVVPIFIGQLETQAILIGLGRVEMPRPLTHDLILSMLAT
                     LEASLVRIEIRDLRDRTFFANVVLRGLEGEVSIDARPSDAIALAVRTTAEVFVSEDIV
                     EMAGVPVDMIRESPPDEAPGSGGLDTAPEADSSPIIEDVSVLRQGSPEFERAVLSAEL
                     ENAIEAEDYERAARIRDRLKELH"
     gene            629..>2058
                     /locus_tag="PLQ29_11505"
     CDS             629..>2058
                     /locus_tag="PLQ29_11505"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HPG87313.1"
                     /translation="MSRRMTMLALLAFAASWLGAEPIDLSAHEAYVRNGFSLEWVFQA
                     PLPGEAPWVEAPGSPGDRPLSMRDLGLPDAPAHGVFSLERLKPSRYTVMIPFDADDEL
                     LSSRTGVGLFLASVGQNWQIYLNGSLVRDEAFTSGDGTMRVERSVRGALVELDARYLR
                     RGRNQLSIMIAGDPRNAATGLSAGGPYLIDAYGTLESTRREGLKLMLIGIYFFFGLYH
                     AILFASRPKARSYLYFAIAAGLLAVYLFCRTYIVYDVILDTRVIAGLEYASLFLLAPA
                     ILAFFDAERGRPVSVFAKAYAAVFALAAVVRLFAWSDELLSLWQYTIALPALYALAFD
                     VVKPTADALRAGSGAGRRGLPARLAAAIAGGGAVKPLLAIAVVGLAALLDALAGSAGT
                     VSFMDYGYLLLVFGIGATLAGQFVRAHNDSEALGADLARRVAARSRELESTLAAQEGL
                     SVGLSEASERLKARLELSARDTRVAA"
BASE COUNT          282 a          740 c          697 g          339 t
ORIGIN      
        1 cccaagggct cggacctcgt cgtgccgatc ttcatcggcc agctggaaac gcaggccatc
       61 ctgatcggcc tcggccgggt ggagatgcca cgcccgctga cccacgacct gatcctgtcg
      121 atgctggcca cgctcgaggc cagcctggtc aggatagaga tacgcgacct gcgcgaccgc
      181 accttcttcg ccaacgtggt gctgcgcggc ctcgagggcg aggtgtcgat agacgccagg
      241 ccgtccgacg cgatagccct ggcggtcagg acgaccgcgg aggtgttcgt ctccgaggac
      301 atcgtcgaga tggccggcgt ccccgtcgat atgatacgcg agtccccgcc cgacgaggct
      361 cccggctcgg gaggcctcga taccgccccc gaggccgaca gctccccgat catcgaggac
      421 gtatcggtgc tgcggcaggg cagccccgaa ttcgagaggg ccgtgctcag cgccgagctc
      481 gagaacgcca tcgaggcgga ggattacgag cgcgccgcgc gcatccgcga ccggctcaag
      541 gagctgcact aggcccggtc ggccctatcc gtcgcttttt cgcttccatg atacgccgta
      601 ctcgcgatac aatggcccgg gggtcgatgt gtcacgacga atgacgatgc tcgcgcttct
      661 cgcctttgcc gcgtcatggc tcggggcgga accgatagac ttgagcgccc acgaggccta
      721 cgtccgcaac ggcttctccc tcgaatgggt cttccaggcg ccgctgcccg gcgaggcgcc
      781 ctgggtggag gcgcccggct cgcccggcga ccgcccgctg tcgatgcgcg acctgggcct
      841 gcccgacgcc ccggcgcacg gggtcttctc gctcgagcgc ctcaagccga gccgctacac
      901 cgtgatgatt cccttcgacg ccgacgacga gctactctcg tcgcgtaccg gcgtcgggct
      961 cttcctggcg agcgtcggcc agaactggca gatctacctg aacggctcgc tcgtgcgcga
     1021 cgaggccttc acctccggcg acggcacgat gcgggtcgag cggtcggtgc gcggcgctct
     1081 ggtcgagctg gacgcgcgct acctgcgccg cggcaggaac cagctgtcca tcatgatagc
     1141 cggcgacccg cggaacgcgg cgacgggcct gtccgcgggc ggaccctacc tgatagacgc
     1201 ctacggcacg ctcgagtcga cgcgccgcga gggcctcaag ctgatgctga tcggcatcta
     1261 cttcttcttc ggcctctacc acgccatcct gttcgcctcg aggcccaagg cgcgctcgta
     1321 cctgtacttc gcgatcgccg ccggcctcct ggcggtctac ctgttctgcc gcacctatat
     1381 cgtctacgac gtcatcctgg acacccgcgt gatagccggg ctcgagtacg cgtcgctgtt
     1441 cctgctcgcg ccggccatcc tggccttttt cgacgcggag cgcggccgcc cggtatcggt
     1501 cttcgccaag gcctacgcgg cggtcttcgc cctggccgcc gtcgtgcggc tgttcgcctg
     1561 gagcgacgag ctcctgtccc tgtggcagta tacgatagcc ctgccggcgc tgtacgcgct
     1621 ggccttcgac gtggtcaagc cgacggcgga cgcgctgcga gccggctcgg gcgccgggcg
     1681 ccgcggcctg ccggcgaggc tggccgcggc gatcgccggc ggcggagccg tgaagccgct
     1741 cctcgcgatc gccgtggtcg gcctcgcggc cctgctcgac gcgctcgccg gctcggcggg
     1801 tacggtatcg ttcatggatt acggctacct gctcctcgtg ttcggcatag gcgcgacgct
     1861 ggccgggcag ttcgtgcggg cgcataacga cagcgaggcg ctcggcgccg acctggcccg
     1921 ccgcgtggcg gcgcgatcca gggagctcga atcgacgttg gccgcccagg aggggctcag
     1981 cgtcgggctg tccgaggcca gcgagcggct caaagcccga ctggagctat ccgcccgtga
     2041 cacgcgcgtg gcggccag
//
LOCUS       DAOPHV010000513         2057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_77276_length_2057_cov_3.531469, whole genome shotgun sequence.
ACCESSION   DAOPHV010000513 DAOPHV010000000
VERSION     DAOPHV010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77276_length_2057_cov_3.531469"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..1647
                     /locus_tag="PLQ29_11510"
     CDS             247..1647
                     /locus_tag="PLQ29_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPG87314.1"
                     /translation="MEGKLRVLIVNGSPKGEHSLTLQHSLYMLGQEPAVEWTVLQAGE
                     ALSPIDYDESWLRAAIDEIERADAILWSTPVYTMLVPWQLLRLFQLVKEAGRNAVFKG
                     KYATSMMTCFHYYDHLAEAWLRATCEDLGMAYMEGRTADNMDMLRNDHRASMRFFMRE
                     FVEACRSRSPLERKYAPLSDRPSPRFELAATGAIAKDGQKAAPRTVLLTDEYRKDGNL
                     SRMIDAFVDAYPYPVEVVDINDFPYEAGCHGCLRCELVGECDRKDGFQEFYLNLVDTC
                     DALVFGMNLEGRYLRPVWKLFLDRTFSNGHRTSMMGKHTAYLVAGPLRSLPNVRQFLE
                     GKDNVGRENCMAIIGDEYDDSALLGRLLRDGAVRLARAIDGRYQKSVNFLGWGGLKIF
                     RDLIYGMRGVVRDDHRFYKTHGLYDFPQKDIPKQLFNLAMGLAFTLKPVRVQAFERMP
                     SMYILLHKRIVDSGKP"
     gene            1760..>2057
                     /locus_tag="PLQ29_11515"
     CDS             1760..>2057
                     /locus_tag="PLQ29_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87315.1"
                     /translation="MRATLLTVCLIVLFNVVGCDLPNASDDNPIADQNPNEAAVAIDI
                     LPAFSGSIRNPRSVIPAGSTAAQVLYSLFTPPLTRFTAAPTSFKVGDSLEVTILG"
BASE COUNT          407 a          670 c          591 g          389 t
ORIGIN      
        1 cctggaggcc cggcgcgacc tcgaactgat cgcggccggc ttcaacgacc tcctggagcg
       61 gtctgccacg gtcagaacgc tcagaccgta gcggcgcctg catgcgggac ctcgacgagg
      121 ccatctcggg cctccggcgt taggaggcgg ctcctaagcg gctagccgcc gatagtccga
      181 acttgactgc gtcgccctcc tgatccacac tcgtagcatc gattctccat ggagaaagga
      241 tataaaatgg aaggcaagtt acgagtcctc atcgtcaacg gcagtcccaa gggcgagcac
      301 agcctgaccc tgcagcacag cctctacatg ctcggacagg agccggccgt cgaatggacg
      361 gtgctccagg ccggcgaggc cctgagcccc atcgactacg acgagtcatg gctacgcgcg
      421 gcgatcgacg agatcgagcg cgccgacgcg atcctctgga gcacgccggt ctataccatg
      481 ctggtgccct ggcagctcct acgtctcttc cagctcgtca aggaagccgg acggaacgcc
      541 gtctttaaag gcaagtacgc gacgagcatg atgacctgct tccattatta cgatcacctg
      601 gccgaggcct ggttacgcgc cacctgcgag gacctcggca tggcctatat ggaaggccgc
      661 acggccgaca acatggacat gctccgcaac gaccaccgcg cgagcatgcg cttcttcatg
      721 cgcgagttcg tcgaggcttg ccgttcgcgg tcgccgctcg agcggaagta cgcgccgcta
      781 tcggatcggc cgtcgccgcg cttcgagctg gccgctaccg gcgccatcgc gaaggacggc
      841 cagaaggccg cgccgaggac cgtgctcctg accgacgagt accgcaagga cggcaacctg
      901 tcgcgcatga tcgacgcctt cgtcgacgcc tatccgtatc cggtcgaggt cgtcgatatc
      961 aatgactttc cctacgaggc cggctgccac ggctgcctcc gctgcgagct ggtcggcgag
     1021 tgcgaccgca aggacggttt ccaggaattc tacctgaacc tcgtcgatac ctgcgacgcc
     1081 ctggtcttcg gcatgaacct cgagggccgg tacctgcgcc cggtctggaa actgttcctg
     1141 gaccggacct tctcgaacgg gcaccgcacg agcatgatgg gcaagcatac cgcctatctc
     1201 gtcgccgggc ccctgcgctc gctcccgaac gtacgccagt tcctcgaggg caaggacaac
     1261 gtcggccgcg agaactgcat ggccatcata ggcgacgagt acgacgactc ggccctgctc
     1321 gggcgcctcc tccgcgacgg cgccgtccgg ctggctcgcg ccatcgacgg gcgctaccag
     1381 aagagcgtca acttcctcgg ctggggcggc ctcaagatct tccgcgatct gatctacggc
     1441 atgcgcggcg tggtccggga cgaccatcgc ttctataaga cgcacggcct gtacgacttc
     1501 ccccagaagg atatacccaa gcagctgttc aacctcgcca tgggtctcgc gttcaccctc
     1561 aagccggtac gtgtccaggc cttcgagcgc atgccctcga tgtacatact gctccataag
     1621 cgcatcgtcg actcggggaa gccttgaaat tgaagcgtga taaagcgaaa ccggcttatg
     1681 aactatcgaa tccatagttc aaaaaacgtg gcttgtaact ctatgtatcg gcttcctata
     1741 ttgctctaaa ggagccttca tgagagctac attgcttacg gtttgcttga tcgtactgtt
     1801 taacgtcgtc ggttgcgatt tgcccaacgc tagcgatgat aatccgatcg ccgatcagaa
     1861 tccgaatgag gctgcggtag cgatcgatat cctacctgca ttctctggca gtattcggaa
     1921 cccacgatcg gtcatcccgg caggatcgac cgccgcgcag gtcttatact cgttgttcac
     1981 gcctcccctg acgaggttca cggcagctcc gacgtcattc aaggtcggtg acagcctcga
     2041 agtaaccata ctaggaa
//
LOCUS       DAOPHV010000514         2056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_77311_length_2056_cov_4.720640, whole genome shotgun sequence.
ACCESSION   DAOPHV010000514 DAOPHV010000000
VERSION     DAOPHV010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77311_length_2056_cov_4.720640"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2056)
                     /locus_tag="PLQ29_11520"
     CDS             complement(<1..>2056)
                     /locus_tag="PLQ29_11520"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPG87316.1"
                     /translation="ADIEARALPDGGWASGRVEVSLSVGSAFDPATAETPPGTAPTDY
                     RRAAEEPDAPPESRPESTGWTVRLALYGPFARSPGGMESPPAEPVAEAEAFVDHAYRD
                     SVWQARATLSLDAPEPWSDELPSLYALVATLVKPDGPGSADEHASLRLGFRTVEVRDR
                     QLLLNGQPVMMRGVNRHEHDERAGKTLGLESMVRDIELMKRANFNAVRLSHYPNDERW
                     YELCDEYGLYLVDEANLESHAYYDHLCRDPRWASAFLERGARMLLRDRNHPSVILWSL
                     GNESGYGQNHDAMAAWMRRADPTRPIHYEGCMRPEWGQGRHDIESVKRGAGCSDLVST
                     MYPPLSLLDEWSRTTSDDRPFIMCEYSHAMGNSNGGLADYWELIESRHGLQGGFIWDW
                     VDQGIEAFDSEGVKYWKYGGDFGDAPSDLDFICNGLVFPDRSPKPALAECAWLQRPVS
                     VASDHPTTGRVRVTNKRYFSSIDELELRWYALADGLPAASGSAKLPHIEPRESAELVL
                     WTSLDLGAREALAGSEAILRLEFRLARDTAWATRGHLVAWEQLPMNDASVTPRPVASR
                     SGARSVAAAKPEATALEKAGGAWRAESRTRDDARLGARVSPDGFLDSLSLDGEPLIIA
                     PLVMELWRAPTENDGLKLFMDKRGLPDYLFYYEHKAMYEWLDAGLDELRFSPPRFERP
                     TDD"
BASE COUNT          292 a          663 c          776 g          325 t
ORIGIN      
        1 gtcgtcggtc gggcgctcga agcgcggtgg ggagaatcgt agctcgtcca ggccggcgtc
       61 gagccattcg tacatggcct tgtgttcgta gtagaagagg tagtcgggca ggccccgctt
      121 gtccatgaag agcttgagtc cgtcattctc ggtcggcgcc cgccacagct ccatgaccag
      181 gggcgcgatg atgagcggct ctccgtcgag gctgagcgag tccaggaagc cgtccggcga
      241 gacgcgagcc ccgagcctgg cgtcgtcccg ggtacgcgac tcggctcgcc aggctcctcc
      301 ggctttctcg agcgctgtgg cctcgggctt cgcggcggcg acggaacggg cgcccgaacg
      361 ggaggctacg ggccgaggcg tgacgctggc gtcgttcatc ggtagctgtt cccaggcgac
      421 gaggtggccc ctcgtcgccc aggccgtatc ccgggccagg cggaactcga gccgtaggat
      481 ggcctctgaa ccggccaggg cctcgcgggc tcccaggtcg agcgaggtcc agagcacgag
      541 ctcggccgac tcgcgcggct ctatatgcgg aagcttggcc gagccggagg ccgcaggtag
      601 gccgtcagcg agcgcgtacc agcgcagctc gagctcgtcg atggagctga agtaccgttt
      661 gttggttacg cgaacgcggc ccgtggtcgg gtggtcggag gcgaccgaga ccgggcgctg
      721 gagccaggcg cactcggcca gggccggctt cgggctacgg tcggggaata cgaggccgtt
      781 gcagatgaag tccaggtcgg acggcgcgtc gccgaagtcg ccgccgtact tccagtattt
      841 gacgccctcg ctgtcgaagg cctcgatgcc ctggtcgacc cagtcccaga tgaagccgcc
      901 ttggaggcca tggcgcgact cgatgagctc ccagtagtcg gccaggccgc cgttggagtt
      961 gcccatcgcg tgcgagtact cgcacatgat gaagggccgg tcgtcgctcg tggtcctgga
     1021 ccactcgtcg agcagggaca aaggcgggta catcgtcgag acgaggtcgg agcagcccgc
     1081 gccgcgcttg accgactcga tgtcgtggcg gccctggccc cactcgggac gcatgcagcc
     1141 ctcgtagtgt atcggcctcg tcgggtcggc gcgccgcatc cacgcggcca tggcgtcgtg
     1201 gttctggccg tagccggact cgttgccgag cgaccagagt atgaccgacg ggtggttgcg
     1261 gtcgcgcagc agcatccgag cgccgcgctc gaggaaggcc gaggcccagc gcgggtcgcg
     1321 gcacaggtgg tcgtagtagg cgtggctctc gaggttggcc tcgtcgacga ggtagaggcc
     1381 gtactcgtcg cacagctcgt accagcgctc gtcgttcggg tagtgggaca ggcggacggc
     1441 gttgaaattg gcgcgcttca tgagctcgat gtcgcggacc atcgactcga ggcccagggt
     1501 cttgccggcg cgctcgtcgt gctcgtggcg gttgacgccg cgcatcatga cgggctggcc
     1561 gttcaggagt agctggcggt ccctgacctc gaccgtgcgg aagcccaggc gcagggaggc
     1621 gtgctcgtcg gcgctacccg ggccgtctgg cttgacgagg gtcgcgacga gggcatagag
     1681 gctcggcagc tcgtcggacc agggttcggg cgcgtcgagg ctcagcgtcg cgcgggcctg
     1741 ccagacggag tcgcggtagg catggtcgac gaaggcctcg gcctcggcta ccggctcggc
     1801 gggcgggctc tccatcccgc ccgggctccg ggcgaagggg ccgtacaggg ccaggcgcac
     1861 ggtccagccc gtcgactcgg gccgcgattc gggcggagcg tccggctcct cggcggcccg
     1921 cctatagtcg gtcggagcgg ttcccggcgg cgtctcggcg gtggccgggt caaaggcgga
     1981 accgacgctt aaggagacct cgacgcggcc cgaggcccag cccccgtccg gcagggcccg
     2041 cgcctcgata tcggcg
//
LOCUS       DAOPHV010000515         2055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_77375_length_2055_cov_3.464500, whole genome shotgun sequence.
ACCESSION   DAOPHV010000515 DAOPHV010000000
VERSION     DAOPHV010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77375_length_2055_cov_3.464500"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1806)
                     /locus_tag="PLQ29_11525"
     CDS             complement(22..1806)
                     /locus_tag="PLQ29_11525"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPG87317.1"
                     /translation="MTPDQLRSKYLDFFKSKHHAVISGKSLIPENDPTVLFTTAGMHP
                     LVPYLLGEPHPAGARLVDYQKCVRTGDIDEVGDPAHLTCFEMLGNWSLGDYFKRDSIA
                     YSWEFLTSPDWLALDPRMLSVTVFQGDDNAPRDDESAGYWKAVGLPEERIAYLPASDN
                     WWAAGPTGPCGPDTEIFYWVGEGLPPAGSNKGTDPAHWMEIWNNVFMQFNRVDEKTLQ
                     KLPKQNVDTGMGLERTVCVLNGKTSVYQTESFQPIIAAIEAVSGYAYGTDPARDPSVR
                     IIADHVRSATMILGDPKGVTPSNVGAGYVLRRLIRRAVRHARKLGIEGGFLGKPAAVV
                     VDLLSGAYPELAENRAKIIEELAREEAKFLETLQKGEHEYEKMLPNLLRNPQKVMSGR
                     LAFKLYDTYGFPIELTQELAAESGMTVDLAEFDASFKKHQDLSRAGAEQTFKGGLADQ
                     SEQATKYHTATHLLHKALRMVLGDHVAQKGSNITAERLRFDFSHSAPMTPDEVARVQA
                     IVNEQIARDLPVTMEMMSLDDAKASGAMALFGEKYESVVKVYTIGDFSKEVCGGPHVE
                     RIGALGRFVIQKEQSSSAGVRRIRAVLE"
BASE COUNT          299 a          617 c          768 g          371 t
ORIGIN      
        1 gtcccgctcc gcggcgcgtc gctactcgag cacggctcgt atccggcgga cgccggccga
       61 cgagctctgc tccttctgga tgacgaacct gcccagggcg ccgatgcgct cgacgtgggg
      121 gccgccgcag acttccttgg agaagtcgcc gatggtgtag accttgacga ccgactcgta
      181 cttctccccg aacagggcca tggcgccgga ggccttggcg tcgtcgaggc tcatcatctc
      241 catggtcacg ggcaggtcgc gggcgatctg ctcgttgacg atggcctgga cgcgggccac
      301 ctcgtcaggc gtcatcgggg ccgaatggga gaagtcgaag cgcaggcgct cggcggtgat
      361 gttggagccc ttctgggcca cgtggtcgcc taagaccatg cgcagggcct tgtggaggag
      421 gtgcgtggcg gtgtggtact tggtcgcctg ctcggactgg tcggcgaggc cgcccttgaa
      481 ggtctgctcg gcgccggccc gcgacaggtc ctggtgcttc ttgaaggagg cgtcgaactc
      541 cgccaggtct acggtcatgc cgctctcggc ggcgagttcc tgggtcagct cgatcgggaa
      601 gccgtaggtg tcgtacagct tgaaggcgag ccggccggac atgaccttct gcgggttccg
      661 cagcaggttc gggagcatct tctcgtattc gtgctcgccc ttctgcaggg tctcgaggaa
      721 cttggcctcc tcgcgggcca gctcctcgat gatcttggcg cggttctcgg ccagctcggg
      781 gtaggcgccg gagagcaggt cgacgacgac ggcggctggc ttgccgagga agccgccctc
      841 gatgccgagc ttcctcgcgt ggcggacggc gcggcggatg agccgcctga gtacgtagcc
      901 ggcgccgacg ttggacgggg tgacgccctt ggggtcgccc aggatcatcg tcgcggagcg
      961 gacgtggtcg gcgatgatgc gcacggacgg gtccctggcg gggtcggtcc cgtaggcgta
     1021 gccggagacg gcctcgatgg cggcgatgat gggctggaag ctctcggtct ggtagacgct
     1081 ggtcttgccg ttgagcacgc agacggtgcg ctcgaggccc atgccggtgt cgacgttctg
     1141 cttggggagc ttctggaggg tcttctcgtc gacgcggttg aactgcatga agacgttgtt
     1201 ccagatctcc atccagtggg ccgggtcggt gcccttgttg gagccggccg gcggcaggcc
     1261 ctcgcccacc cagtagaaga tctcggtatc gggcccgcag gggccggtcg ggccggcggc
     1321 ccaccagtta tcggaggccg gcaggtaggc gatgcgttcc tcgggcaggc cgacggcctt
     1381 ccagtagccg gccgactcgt cgtcgcgggg agcgttgtcg tcgccctgga ataccgtgac
     1441 cgacagcatg cgcgggtcga gggcgagcca gtcggggctg gtcaggaact cccaggaata
     1501 cgctatcgag tcccgcttga agtagtcgcc gagcgaccag ttgccgagca tctcgaagca
     1561 ggtcagatgc gcggggtcgc cgacctcgtc gatgtcgccg gtgcgcacgc acttctggta
     1621 gtcgaccagg cgggcgccgg cggggtgcgg ctcgccgagc aggtagggca cgagggggtg
     1681 catgccggcg gtggtgaaca gcacggtcgg gtcgttctcg gggatgagcg acttgcccga
     1741 gatgacggcg tggtgcttcg acttgaagaa atccaggtac ttcgagcgca actggtccgg
     1801 tgtgatcata gtgggacgat agtaataagg aggcgagccc tcggtcaatg gaccatcgcg
     1861 cctttcgagc gcgccgagcc tatcggctcc gcgccagtag ggcgccgcgc ccgaagggcg
     1921 cgactagtcc tcgtccgggt gacgctcgcg gatgtcgtgg atgcggggga ggacctcgaa
     1981 gaaggactcg acgaggtctg ggtcgaactt gccgcccgag cacgagcgta tctcggcgag
     2041 cacgttgtcc tcggt
//
LOCUS       DAOPHV010000516         2035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_78467_length_2035_cov_4.134848, whole genome shotgun sequence.
ACCESSION   DAOPHV010000516 DAOPHV010000000
VERSION     DAOPHV010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78467_length_2035_cov_4.134848"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..1667
                     /locus_tag="PLQ29_11530"
     CDS             33..1667
                     /locus_tag="PLQ29_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG87318.1"
                     /translation="MINVSDLKLGFGERILFKDVNLKFVPGNCYGVIGANGAGKSTFL
                     KLLSGELEPDFGQISYPPKLRMAVLKQDHFEFEDERLVDVVIRGYGKLYSIMKEREAI
                     YAKEDFSDADGIRASELEGEFADLGGWEAEAQASILLSGLGVPETDHEKPMREVSESI
                     KVRALLAQALFGNPDILLLDEPTNGLDLDSISWLEDFLMDFQNIVIVVSHDRHFLNAV
                     CTQILDIDFGLIRQYPGNYDFWYKVSKMLQAQMKDEKKRREDKIKDLKEFIQRFASNA
                     AKSRQATSRRKVLDKLEVDDLQATSRRFPWVGFKPERDPGNNVVAVRGLTKIEDGKAV
                     LSGLDLTVNRGDKIAFVGREHLAKTALFRILSGEDEDYEGECSWGQTMSSSYFPKENA
                     ALFDSDDSIVDWLKRYTSSDDETYIRSFLGRMLFSGDEALKPVRVLSGGEKVRCLLSR
                     MMLQAANCLILDEPTNHLDLDSITALNNGLIEFPGVVLFTSHDHEFVNSIANRVVEIC
                     PGGVIDRLISFDEYLRDEQVKALRDQYYHGHVIAEI"
BASE COUNT          423 a          658 c          596 g          358 t
ORIGIN      
        1 actacggaat cttacaccgc aaggaagctc tcttgatcaa tgtcagcgac ctaaagctcg
       61 gcttcggcga gcgcatactc ttcaaggacg tcaacctcaa attcgtgccg ggcaactgct
      121 acggcgtgat aggcgccaac ggcgccggta agtccacctt cctcaagcta ctgtccggcg
      181 aactcgagcc ggacttcggc cagatatcct acccgcccaa gctgcgcatg gcggtgctta
      241 agcaggacca cttcgagttc gaggacgagc gcctcgtcga cgtggtcatc cgcggctacg
      301 gcaagctcta ctcgatcatg aaggaacgcg aggccatcta cgccaaggag gacttcagcg
      361 acgccgacgg catcagggcc agcgagctcg agggcgaatt cgccgaccta ggcggatggg
      421 aagccgaggc gcaggcgtcc atcctgctgt cgggcctcgg cgtgccggag accgaccacg
      481 aaaagccgat gcgcgaggtg tcggagagca tcaaggtgcg cgccctcctc gcccaggccc
      541 tgttcggcaa cccggatatc ctgctcctcg acgagccgac gaacggcctg gacctcgaca
      601 gcatctcgtg gctcgaggac tttctgatgg acttccagaa tatcgtcatc gtcgtctccc
      661 acgaccgcca cttcctcaac gccgtctgca cccagatact cgatatagac ttcggcctca
      721 tccgccagta ccccggcaac tacgacttct ggtacaaggt ctccaagatg ctccaggcgc
      781 agatgaagga cgagaagaag cgccgcgagg acaagataaa ggacctcaag gagttcatcc
      841 agcgcttcgc gtcgaacgcg gccaagagcc gccaggcgac gagccgccgc aaggtgctcg
      901 acaagctcga ggtcgacgac ctgcaggcga cgagccggcg cttcccctgg gtcggcttca
      961 agcccgagcg agacccgggc aacaacgtcg tcgccgtccg cgggctcacc aagatcgagg
     1021 acggcaaggc cgtgctgtcc gggctcgacc tgaccgtgaa ccgcggcgat aagatagcct
     1081 tcgtcggccg cgagcacctg gccaagaccg ccctgttccg catcctgtcc ggcgaggacg
     1141 aggactacga gggcgagtgc tcgtggggcc agacgatgag tagctcgtac ttcccaaagg
     1201 agaacgcggc gctgttcgac tcggacgact ccatcgtcga ctggctcaag cgctatacct
     1261 cgagcgacga cgagacctac atccgctcct tcctcgggcg catgctgttc tccggcgacg
     1321 aggcgctcaa acccgtgcgc gtgctatccg gcggcgagaa ggtgcgctgc ctcctctcgc
     1381 gcatgatgct ccaggccgcc aactgcctga tactcgacga gccgaccaac cacctggacc
     1441 tcgactcgat caccgccctc aataacggac tgatcgagtt ccccggcgtc gtactgttca
     1501 cgagtcacga ccacgagttc gtgaactcca tcgccaatcg cgtcgtcgag atctgccccg
     1561 gcggcgtcat cgaccgcctg atcagcttcg acgagtacct ccgggacgaa caggtcaagg
     1621 cgctcagaga ccagtactac cacgggcatg tgatagccga aatctagtaa aagcggacga
     1681 gtacgccgct tttactaccc gtagttgaag ctgactcgct tcgctcgccg cttaactacg
     1741 gggcggttag gcgtaggggc gcggccctac gggcgcgacg cgacttacgg actcgcttcg
     1801 ctcgccgtcg ccgcggtggt ggcagactcg cttcgctcgt cgtgaccttg ggcatagatg
     1861 caacaaggat acgtggtgga tgtaacaagg atacggcttg gtactaataa ggttctgtcg
     1921 aagcccggcc tgcttgagcg ttacgtccag atccttaatc gctctctata tcctgcgata
     1981 ctgaaccgcc ccgggattgt cggagactaa aatctttgag aggatacagt catgg
//
LOCUS       DAOPHV010000517         2034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_78552_length_2034_cov_2.588681, whole genome shotgun sequence.
ACCESSION   DAOPHV010000517 DAOPHV010000000
VERSION     DAOPHV010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78552_length_2034_cov_2.588681"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PLQ29_11535"
     CDS             complement(<1..747)
                     /locus_tag="PLQ29_11535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPG87319.1"
                     /translation="MARKNLLKGFKRPKGITYEQSESTESYGKFLAYPFEPGYGTTVG
                     NTLRRILLSSIQGYAITAVRITRHDADGAQHVVSSEFETIPGVSEDTIELLNNLKRLR
                     VKLADDEEQAVFAYEFNGPADITGADLMADKSLSILNPGVKLMSMAQGVKLEVEIQID
                     LGRGYVPSEVNERYIDVVGTIPMDAIFTPVTKARYSVEPTRVGHRSDYDKLVLEIWTD
                     GTLKPEDALAEAAKIAKDHFSIFVNFDEGEI"
     gene            complement(757..1392)
                     /gene="rpsD"
                     /locus_tag="PLQ29_11540"
     CDS             complement(757..1392)
                     /gene="rpsD"
                     /locus_tag="PLQ29_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPG87320.1"
                     /translation="MARYIGPRCRLCRAEGKRLFLKGDKCKSDKCPVNRKRMAPGKDP
                     KTRQKKQSEYAVQLREKQKLKRSYGLMEKQFKLTFEKAMRMPGKTGENLFALLERRLD
                     SVVYRLRFATSRSQARQIVLHGHILVNGKRVDVPSYQVKTGDTITVHQSYKTNKVLLS
                     ALAEYEKSGVMPWVSLDVDKLEGKFNVVPQRQDIVDMADIREQLVVELYSK"
     gene            complement(1410..1790)
                     /gene="rpsK"
                     /locus_tag="PLQ29_11545"
     CDS             complement(1410..1790)
                     /gene="rpsK"
                     /locus_tag="PLQ29_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPG87321.1"
                     /translation="MAQTKKRKEKKSVYEGNVYIQATFNNTIITVTDLNGNAISWGSS
                     GGLGFRGAKKSTPFAAQSVTEQAVAKALNVGLREVHVYVKGPGVGRESAIRQLGALGL
                     KIKSISDVTPIPHNGCRPPKARRM"
     gene            complement(1805..>2034)
                     /gene="rpsM"
                     /locus_tag="PLQ29_11550"
     CDS             complement(1805..>2034)
                     /gene="rpsM"
                     /locus_tag="PLQ29_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPG87322.1"
                     /translation="SNEEVNELRKLIENEYKVEGRLRSETALNIKRLMDIGCYRGLRH
                     RKGLPVRGQRTRTNARTRKGKKKTVANKKKA"
BASE COUNT          333 a          608 c          591 g          502 t
ORIGIN      
        1 aatctcgcct tcatcaaaat tgacgaagat ggagaaatgg tctttggcga ttttcgctgc
       61 ttcggccagc gcgtcttccg gtttcagggt gccgtcggtc catatttcca gcaccagttt
      121 gtcgtaatcg gatcggtgac cgacgcgggt tggctccacg gagtaacgtg ctttggtaac
      181 cggcgtaaag attgcatcca tgggtatggt gccaaccacg tcgatgtatc gctcgttcac
      241 ttcgcttgga acatagccgc ggcctaggtc gatctgaatc tcgacctcca gcttaacgcc
      301 ctgggccatg ctcatgagct tgacgccggg gttgagtatg gacaggctct tgtcggccat
      361 aaggtcggcg ccggtgatgt ctgccgggcc attgaactcg tatgcaaata cggcttgctc
      421 ttcgtcgtcc gcaagtttaa cccgcaaacg cttcaggttg ttcaacagct cgatggtatc
      481 ttcggaaacg ccgggtatgg tctcgaactc ggaggacact acgtgctgcg ctccgtcggc
      541 atcatgcctg gttatgcgca ccgccgtgat cgcgtagccc tgtatggaag acaggagtat
      601 gcgacgcagg gtgttgccaa cggtcgttcc gtagccgggc tcaaagggat acgcgagaaa
      661 cttcccgtaa ctttcggtac tctcgctctg ctcgtacgtg atgcctttcg gcctcttgaa
      721 tcccttgagc aggttctttc gcgccatgtc cactccttac ttcgaataga gctctaccac
      781 gagctgttcg cggatatcgg ccatgtcgac aatgtcctgg cgctgcggga cgacgttgaa
      841 cttgccttcg agcttgtcca cgtcgagcga gacccagggc atgacgccgc tcttctcgta
      901 ttcggccagg gccgagagaa ggaccttgtt ggtcttatag gactggtgca cggtgatcgt
      961 gtcgccggtc tttacctggt aggacggtac gtccacacgc ttgccgttga cgaggatgtg
     1021 cccgtggagc acgatctgcc tggcctgcga gcggctcgta gcgaaacgga gcctgtagac
     1081 gacgctgtcg agcctgcgct cgagcagggc gaacaggttc tcgccggtct taccaggcat
     1141 gcgcatggcc ttctcgaagg tcagcttgaa ctgcttctcc atgaggccgt aagacctctt
     1201 gagcttctgc ttctccctga gctggacggc gtactcggat tgcttcttct gcctggtctt
     1261 cgggtccttg ccgggcgcca tgcgcttgcg gttgaccggg cacttgtcgc tcttgcactt
     1321 gtcgcccttc aggaaaagcc tcttaccctc ggcgcggcag agcctgcagc gagggccaat
     1381 gtaacgtgcc attctttttc tcccctacct tacatcctgc gcgctttcgg ggggcggcag
     1441 ccgttgtgcg gaatgggcgt cacgtcgctg atgctcttga tcttgagccc cagggcgccg
     1501 agctgccgga tcgcggactc gcgaccgact cccgggccct tgacgtagac gtgcacttcg
     1561 cggaggccca cgttgagcgc cttcgccact gcctgctcgg tgaccgactg cgccgcgaag
     1621 ggggtggatt tcttcgcgcc gcggaagccg aggccaccgg aggagcccca cgatatagcg
     1681 ttaccgttca aatcggttac ggtgatgatg gtattgttga aggtcgcctg gatatagacg
     1741 ttaccctcat atacgctctt cttctctttg cgtttcttgg tctgagccac gagccctacc
     1801 ctccttaggc cttcttctta ttggccacgg tcttcttctt acccttgcgc gtgcgcgcgt
     1861 tcgtccgcgt gcgctggcct cggacgggca ggcccttccg gtgcctgagg ccccggtagc
     1921 aacctatatc catgagccgc ttgatattga gggccgtctc cgagcggagc cttccctcga
     1981 ccttatactc gttctcgatc agcttgcgaa gctcgttgac ttcctcgttc gaca
//
LOCUS       DAOPHV010000518         2030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_78778_length_2030_cov_3.076456, whole genome shotgun sequence.
ACCESSION   DAOPHV010000518 DAOPHV010000000
VERSION     DAOPHV010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78778_length_2030_cov_3.076456"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="PLQ29_11555"
     CDS             complement(<1..1533)
                     /locus_tag="PLQ29_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87323.1"
                     /translation="MRRRMLIEENLLISAALSIATLVVVLGSFFLIAGRHEIDGIRLQ
                     SEATADEIASILIEPLYNIDDNQAARIAAALVASGRIDSLRLESTVSGVIFEQRAATP
                     PRWIQAQDRRIERGELHLGDVRIAVSEREIRETLSLYMLASGLLAVATVIVNATVNLF
                     FIRRRVRKAFVDFEEGIRQINGGAFGYEIPPSGYHDLDHLILSFNRMSRSVALSNAQL
                     SSLNAELEERVTERTSELRTALAEQDKLRERLVEAAKMSVLGRLSAGVAHEINTPLGA
                     IVSSNGYLIEFIEDEYRSQLEYFAGLPALEREAYERLLTIGVAGNRGLSASMASSAER
                     RSFAEALSSAGVPDAEAIADELADLSLHGERLAVVDALSRCGGPLELTRRVAGVINAY
                     RMTRVIEEAGGKAAAVVSALRSHIGAFQDEPRDVDVHDEVRRSLTLMHGAIKYGIETR
                     IELAPGRPMARAVGLDRILINLTRNAAQAMRFRGVMTIRTEIKGDLVIVSIIDTGTGI
                     PPE"
     gene            complement(1623..>2030)
                     /locus_tag="PLQ29_11560"
     CDS             complement(1623..>2030)
                     /locus_tag="PLQ29_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87324.1"
                     /translation="KRAMESLEATASRSQSGFARVLEQTTLVASHDAVIKGAMDEQRS
                     GGSQVLEAIHRIGDITAEVRSGSECILGASAEARQLTGELADMAVEIGSGVREIAEGQ
                     AQIVQAIGRVNGIAADNRRDLDALMARFARFRT"
BASE COUNT          324 a          668 c          641 g          397 t
ORIGIN      
        1 ctcgggcggt atgccagttc ccgtatctat gatagatact atcacgagat caccttttat
       61 ctcggtacga attgtcatga cgcccctgaa gcgcatggcc tgggcggcgt tcctcgtcag
      121 gttgatgagt atgcggtcca atcctaccgc ccgcgccatc ggcctgccgg gggctagttc
      181 gatcctcgtc tcgatgccgt acttgatggc gccatgcatc agcgtcagcg atctgcgtac
      241 ctcatcgtgc acgtcgacgt cgcgcggctc gtcctggaac gcgccgatgt gcgagcgtag
      301 cgccgaaacg acggcggccg ccttgccgcc ggcctcctcg atcacgcggg tcatcctgta
      361 cgcgttgatt accccggcca cgcgcctggt tagctcgagc ggcccgccgc atcgcgacaa
      421 ggcgtcgacc acggccagac gctcgccgtg gagcgatagg tcggccagct cgtcggctat
      481 ggcctcggcg tcggggaccc ccgccgagga tagcgcctcg gcgaaggagc gcctctcggc
      541 ggacgatgcc atgctggcag acaggccgcg gttgcccgct actccgatgg tcaggagccg
      601 ctcgtaggcc tcgcgttcca aggcgggcaa tcccgcaaag tactcgagct gagaccggta
      661 ctcgtcctcg atgaactcga tcaggtagcc gttcgacgac acgatggcgc cgagcggcgt
      721 gtttatctcg tgggccacgc cggccgatag ccgtccgagc accgacatct tggcggcctc
      781 gaccaagcgc tcgcgaagct tgtcctgctc ggccagcgcc gtccggagct ctgaggtgcg
      841 ctcggtcacc cgctcctcga gctcggcgtt caggcttgaa agctgagcgt tggagagcgc
      901 gacgcttcgg ctcatcctat tgaacgagag gatcaggtga tcaaggtcgt gatagccgct
      961 cggaggtatc tcgtagccga acgctccgcc gtttatctgt cgaatccctt cctcgaagtc
     1021 gacgaacgcc ttacggacgc gcctgcgtat gaagaacagg ttgaccgtgg cgttcacgat
     1081 gacggtcgcg accgcgagca ggccgctggc gagcatgtag agcgatagcg tctcgcgtat
     1141 ctctcgctcg cttaccgcga tacgtacgtc gccgaggtgt agctcgccgc gctctatgcg
     1201 ccgatcctgg gcttgtatcc atcgcggggg ggtagccgcc ctctgttcga atatgacgcc
     1261 ggagaccgtc gattccagac gaaggctgtc gatgcgcccg gacgcgacga gggccgccgc
     1321 tatcctggcg gcctggttgt cgtcgatatt gtagagcggc tcgatcagga tgctggctat
     1381 ctcgtcggcc gtcgcctcgg actgtagccg tatgccgtcg atctcgtgcc ggccggctat
     1441 caggaagaac gagccgagca cgaccaccag ggtcgcgatc gacagggccg ccgagatgag
     1501 caggttctct tctatcagca tgcggcgtct catggcggag ttggctcctc ggcgttaaag
     1561 gctttcttta taactatatg tcgccggacg tctggcgcaa gagcccctgc gcggcggtct
     1621 cgtcaggtcc tgaacctggc gaagcgggcc attagcgcgt cgaggtcgcg acgattgtca
     1681 gccgctatgc cgttgacgcg gcctatcgcc tggactatct gcgcctggcc ctcggcgatc
     1741 tccctcacgc ccgagcctat ctccaccgcc atatccgcca gctcgcccgt cagctgccga
     1801 gcctcggcgc tggctcctag tatgcattcc gaaccggaac gcacctctgc cgtgatgtcg
     1861 ccgatacgat ggatggcctc gagcacctgg ctgccgccgg agcgctgctc gtccatggcg
     1921 cccttgatca cggcgtcgtg ggaagccacg agcgtcgtct gctccaatac gcgggcgaag
     1981 cccgactggg atctgctcgc ggtggcctcg agggactcca tggcccgctt
//
LOCUS       DAOPHV010000519         2028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_78891_length_2028_cov_4.564622, whole genome shotgun sequence.
ACCESSION   DAOPHV010000519 DAOPHV010000000
VERSION     DAOPHV010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78891_length_2028_cov_4.564622"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2028)
                     /gene="mfd"
                     /locus_tag="PLQ29_11565"
     CDS             complement(<1..>2028)
                     /gene="mfd"
                     /locus_tag="PLQ29_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPG87325.1"
                     /translation="SLIARDEVAVHPSGLSAGFSVPQAKLAFVHEGEIFGRRKRAPKS
                     LKSVRSKVIDTFVELNPGDFVVHVNYGIGRFVGIERVKVLGHDRDYVKIEYADEESVF
                     VPIEQSNLVQRYIGNEGDEPRMDRLGSKSWENRKNKVKKSVEDLAERLIRIYSRRKLA
                     RGFAFPPDGEWQTAFEAAFPYEETVDQLSCIDDVKRDMESERPMDRLVCGDVGYGKTE
                     IAMRAAFKAVMGGKQVAFLAPTTILAEQHYENCQDRFRGLPVRFGMLSRFVDRKEQKK
                     TLAGVAEGSIDVLVGTHRILQKDVAFKDLGLLIVDEEQRFGVKDKERLKELRANVDCL
                     TLTATPIPRTLHMSMLKIRDLSVLTTPPNNRHPIQTVVDEFQPDLIAKAIRREVERDG
                     QVFFLHNRVQSLDDTMAFIQRLVPEVMVEKAHGQMDARELEDIMHRFIHRGFHVLVST
                     TIIENGIDIPNVNTIIIDRADIYGISQLYQLRGRVGRSDRLAYAYLFYPDGRALSELA
                     MKRLQIISDFTDLGSGFKIAMKDLEVRGAGNLLGREQSGDIYSVGFDLYLKLLDEAVA
                     RLSDEGYKPEEEPYLELEYSGYIPDDYISQQAVKMEIYKKIASIMSQDDLEALYRELE
                     DRFGPVPEDASSLLALAEIRLLCKRLSIATLKERKGFVTVEFSKVSKISVDR"
BASE COUNT          319 a          654 c          653 g          402 t
ORIGIN      
        1 cctatcgacc gatatcttcg agaccttcga gaactcgacg gtgacgaagc ccttgcgctc
       61 cttgagcgtc gctatcgaca ggcgcttgca caggaggcgt atctcggcga gcgcgaggag
      121 gctcgaggcg tcctcgggta ccgggccgaa gcggtcctcg agctcccggt acagagcctc
      181 gagatcgtcc tgggacatga tcgaggcgat ctttttgtag atctccatct tgaccgcctg
      241 ctggctgatg tagtcgtcgg gtatgtagcc cgagtactcg agctccaggt acggttcctc
      301 ctcgggtttg tacccctcgt ccgataggcg ggcgacagcc tcgtcgagca gcttgaggta
      361 caggtcgaag ccgaccgagt agatgtcgcc ggattgctcg cggccgagca ggttcccggc
      421 cccgcgcacc tccaggtcct tcatcgctat cttgaagccc gagccgaggt cggtgaagtc
      481 cgagatgatc tgtagccgct tcatagccag ttccgacagg gctcggccgt cggggtagaa
      541 caggtacgcg taggcgagcc ggtccgaccg acccacgcga ccgcgcagct ggtacagctg
      601 gctgatgccg tagatgtcgg cgcggtcgat gatgatcgta ttgacgttcg gtatgtcgat
      661 gccgttctcg atgatggtcg tggagacgag cacgtggaag ccgcggtgga tgaagcggtg
      721 catgatgtcc tcaagctcgc gcgcgtccat ctgcccgtgg gccttctcga ccatcacctc
      781 cggcacgagc cgctggatga aggccatcgt gtcgtcgagg ctctgtaccc ggttatggag
      841 gaagaagacc tggccgtcgc gctcgacctc ccgccgtatc gccttggcga tcaggtcggg
      901 ctggaactcg tcgaccaccg tctggatggg atgcctgttg ttcggcgggg tcgtcagcac
      961 cgacaggtcc ctgatcttga gcatcgacat gtgcagggtc ctcggtatgg gcgtcgcggt
     1021 cagcgtgagg cagtcgacgt tggcccgcag ctccttgagc cgctccttgt ccttgacgcc
     1081 gaagcgctgc tcctcgtcga cgatcaggag cccgaggtcc ttgaacgcca cgtccttctg
     1141 caggatgcgg tgcgtcccga cgagcacgtc tatcgagccc tcggcgacgc cggccagcgt
     1201 cttcttctgc tccttgcggt cgacgaagcg cgacagcatg ccgaagcgca caggaaggcc
     1261 cctgaaacgg tcctggcagt tctcgtagtg ctgctccgcg agtatcgtcg tcggcgccag
     1321 gaaggcgacc tgcttcccgc ccatcaccgc cttgaacgcg gcgcgcatcg ctatctcggt
     1381 cttgccgtac ccgacgtcgc cgcagaccag gcggtccatc gggcgctcgc tctccatgtc
     1441 gcgcttcacg tcgtcgatgc acgacagctg gtcgaccgtc tcctcgtagg ggaaggccgc
     1501 ctcgaaggcg gtctgccact cgccgtcggg cgggaacgcg aagccccggg ccagcttgcg
     1561 cctcgagtag atgcgtatca gccgctcggc gaggtcctcg accgacttct tgaccttgtt
     1621 cttgcggttc tcccaggatt tggagccgag ccggtccatc ctcggctcgt cgccctcgtt
     1681 gccgatgtag cgttggacca ggttcgactg ctcgatcggg acgaagacgc tctcctcgtc
     1741 ggcgtactct atcttgacgt agtcgcggtc gtggccgagc accttgacgc gctcgatgcc
     1801 gacgaagcgg ccgatgccgt agttgacgtg cacgacgaag tcgcccgggt tgagctcgac
     1861 gaaggtgtcg atcaccttcg accgtacgct cttgagcgac ttgggcgcgc gcttgcggcg
     1921 cccgaagatc tcaccctcgt gcacgaaggc cagcttggcc tgcggcacgg agaagccggc
     1981 cgacagcccg ctcgggtgca ctgccacctc gtcgcgcgcg atcagcga
//
LOCUS       DAOPHV010000520         2025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_79065_length_2025_cov_4.947208, whole genome shotgun sequence.
ACCESSION   DAOPHV010000520 DAOPHV010000000
VERSION     DAOPHV010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79065_length_2025_cov_4.947208"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PLQ29_11570"
     CDS             complement(<1..733)
                     /locus_tag="PLQ29_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87326.1"
                     /translation="MRSPATRVLVLMLALGTAPTWAQTLAGASDGLYRVEGPSRATRL
                     LSGVEVKKIVRAGDGFYFMTSRGVLYSRDLSGFEERNAGFPVKTIKTYADGKKGFETE
                     VQELKDLEADPYDPATLVACTKDQVFLTRDGGLSWKAYPSPAPQPGMKAVAVTSMPEL
                     LVFASHPIKGPFVMAGSGTWREIGGDLGRSDPSGGPDEISDIVVEAGADGPVVWAANS
                     FLPRLYRYDHSARAFRVQYAGREDFA"
     gene            complement(787..1566)
                     /locus_tag="PLQ29_11575"
     CDS             complement(787..1566)
                     /locus_tag="PLQ29_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87327.1"
                     /translation="MDRRAYDRFSELRDEFKARVAAWSAALPGLADAQRRLAVETGDT
                     GYAVETPIVYNRALDEVGPGDDIAWLVVADNPGKREQEAAMNRYLVGRSGQAAERFFA
                     RELGIDFRRQVVVVNKTPVHTPKTAQLRKLARIIPEAGPVLEESQRYMAALVPELQAC
                     LGARVWVMGLSELRPKGLFEAWRAALNEAYRLADADALGRSLPAPSKGLYGFNHFSMG
                     SFATDLKRRRASGEPVVDAVLRIGAENRPRLLDLSTDAPFL"
     gene            1714..>2025
                     /locus_tag="PLQ29_11580"
     CDS             1714..>2025
                     /locus_tag="PLQ29_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG87328.1"
                     /translation="MSRIIVFVNQKGGVGKTTSVINLGAYLARAGKRTLLVDFDPQAN
                     LTSGVGGDKAAPGAYDVISGRAAMRDVIKPTKVDGLFLAPSSIALSGATVELDGGAGQ
                     SD"
BASE COUNT          323 a          687 c          680 g          335 t
ORIGIN      
        1 aagcgaaatc ctctcggccc gcgtattgga ccctgaaggc gcgcgccgag tggtcgtagc
       61 ggtagagcct gggcaggaac gagttggccg cccagacgac ggggccgtcg gcgcctgcct
      121 cgacgacgat gtccgatatc tcgtcgggac cgcccgaggg gtcgctgcgg ccaaggtcgc
      181 cgccaatctc gcgccaggtg cccgaaccgg ccatcacgaa gggtcccttg atcgggtgcg
      241 acgcgaagac gagcagctcg ggcatgctcg tgacggccac cgccttcatg cccggctggg
      301 gggccggcga cggataggcc ttccagctca agccgccgtc tcgggtcagg aagacctgat
      361 ccttggtgca ggcgacgagc gtcgctggat cgtaggggtc ggcctccagg tccttgagct
      421 cctgtacctc cgtctcgaag cccttcttgc cgtcggcgta ggtcttgatc gtcttgaccg
      481 ggaaaccggc gttgcgctcc tcgaagccgg acaggtcgcg gctgtacagc acgccgcggc
      541 tggtcatgaa atagaagcca tcaccggccc tgacgatctt cttgacctcg acgccggaca
      601 gcaggcgggt ggccctggac gggccttcga cacggtagag gccgtccgag gcccccgcga
      661 gggtctgggc ccaggttggg gcggtgccga gggcgagcat caggactagt acgcgggtgg
      721 cgggcgatcg catggggtaa ttctccgagt cggcgatgat cgagggcgct ctggccaacg
      781 tcgatactac aaaaaagggg cgtccgtgga taggtcgagc agcctagggc ggttctcggc
      841 gccgatgcgc agtaccgcgt cgacgacggg ctctcccgac gcccgtcgtc gcttgagatc
      901 ggtcgcgaag ctacccatcg agaaatggtt gaagccgtac agccccttcg acggcgctgg
      961 caggctgcgc ccgagcgcgt cggcgtcggc caggcggtag gcctcgttca gcgcggcccg
     1021 ccaggcctcg aagaggccct tgggtcgcag ctccgatagg cccatcaccc agactctcgc
     1081 cccgaggcag gcctgcagct cgggcacgag ggccgccata tatcgctggc tctcttcgag
     1141 caccggcccg gcctcgggta tgatcctggc aagcttgcgc agctgggccg tcttcggggt
     1201 atggaccggc gtcttgttga cgacgacaac ctggcgcctg aagtcgatgc cgagctcccg
     1261 cgcgaagaag cgctcggcgg cctgaccgga ccggccgacc aggtagcggt tcatggcagc
     1321 ttcctgttcg cgcttgcccg ggttgtcggc caccaccagc cacgcgatgt cgtctcccgg
     1381 cccgacctcg tcgagcgcgc ggttgtagac tatcggagtc tcgacggcgt agccggtatc
     1441 gccggtctcc acggccaggc gcctctgggc gtcggccaag ccgggcaggg cggcggacca
     1501 cgcggcgacc cgcgccttga actcgtccct gagttcgctg aacctgtcgt acgcccgtct
     1561 gtccaccgcg cccgccttcg tacctaagct tcgcaacact aacccgggct ggccccgatc
     1621 cgcaagcgcg ggcgatagtc gatcgtatgc cgtagccctt gccgcgcttt ccatgctatc
     1681 ctgatctata cctgagaacc ccaaggacga acgatgtcca ggataattgt attcgttaac
     1741 cagaagggcg gcgtcggcaa gacgacgtcc gtgatcaacc tcggagccta cctcgcccgc
     1801 gccggcaagc gtacgctgct cgtcgacttc gacccgcagg ccaacctgac cagcggcgtc
     1861 ggcggcgaca aggcggcccc gggcgcctac gacgtcatct ccggccgcgc ggccatgcgc
     1921 gacgtgatca agccgaccaa ggtggacggt ctgttcctgg ccccgtcgtc gatcgctctg
     1981 tccggcgcca ccgtcgagct cgacggtggc gccggacaga gcgat
//
LOCUS       DAOPHV010000521         2023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_79213_length_2023_cov_3.033028, whole genome shotgun sequence.
ACCESSION   DAOPHV010000521 DAOPHV010000000
VERSION     DAOPHV010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79213_length_2023_cov_3.033028"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="PLQ29_11585"
     CDS             complement(<1..1150)
                     /locus_tag="PLQ29_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87329.1"
                     /translation="MSPRPTEYDSCPSADWFEEATESFSDRWDSTPWHDNGERKLAPL
                     KGSLRVHAPSINRLPETFLPSVLDQYLAVLSEGYEYQALDCLRILGDDRYLCANDSGE
                     TFYVWSRSMTINVSEAGYTFLTAISRLRGEGGEPTPAITYGPVLSWKALRAADFSAIG
                     RELARDLYRLQGLSAVIRRDPVPFWAAWALGDAPIMKHGTEELCTCWQTGRFSASPET
                     ALSGSWKRDEVGKRIRLRKPGSKPFFEQVVIYDSKTSLGLVLTRRRGYQKKLIDSLAS
                     VFTPDAEEPCVASAALEMVFSDILKRKLGFSDWTRPFERLDEERIERDRARHPERESE
                     LDSLNDALQALLPHINAHREPDWEAFAAEHDVDAGLIDTLKSFYQKYAR"
     gene            complement(1713..1784)
                     /locus_tag="PLQ29_11590"
     tRNA            complement(1713..1784)
                     /locus_tag="PLQ29_11590"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1750..1752),aa:Cys,seq:gca)
BASE COUNT          357 a          656 c          610 g          400 t
ORIGIN      
        1 ctctggcgta cttctggtaa aagctcttca gcgtatcgat caagcctgcg tcgacatcgt
       61 gctcggccgc gaaggcctcc cagtccggct cccgatgggc gttgatgtga ggcaataatg
      121 cctggagcgc gtcgttcagg ctatccagct cggactcacg ctcgggatgc cgcgctcggt
      181 cgcgttcgat ccgctcctcg tccaatcgct cgaacggtcg ggtccagtcc gagaatccca
      241 gctttcgctt caggatatcg gaaaacacca tttctagggc cgcggaggcg acgcacggct
      301 cctccgcgtc tggagtgaac acggacgcga gcgagtcgat cagcttcttc tgatagccgc
      361 gccgtctcgt cagtacgagg ccgagcgacg ttttggagtc gtagatgacg acctgctcga
      421 agaagggctt ggaaccgggc ttcctgagcc gtattcgctt gccgacttcg tcgcgcttcc
      481 acgatcccga aagcgcggtc tcggggctgg ccgagaaccg gcccgtctgc caacaggtac
      541 agagctcctc ggtaccgtgc ttcatgatcg gagcgtcgcc gagagcccag gcggcccaga
      601 acggtacggg gtcgcgccga atcacggccg atagcccctg cagcctatag aggtcgcggg
      661 ccagctcccg cccgatggcg gagaaatcag cggcgcgcag ggctttccag gataggaccg
      721 gaccgtacgt gatggctgga gtcggctcgc caccttcgcc tctcaggcgg ctgatagccg
      781 tcaagaacgt gtatcccgct tcgctgacgt tgatggtcat cgatcgggac cagacgtaga
      841 acgtctctcc tgaatcattc gcgcacagat agcggtcgtc gcccaggatc cgcaggcagt
      901 cgagcgcctg gtattcgtag ccctccgaca acaccgcgag atactggtcg agcaccgacg
      961 gaaggaaggt ctcgggcagg cggttaatgg atggcgcatg gacgcggagg gatcccttta
     1021 gaggcgccag ctttcgctcg ccgttatcgt gccatggcgt agagtcccat cgatcgctga
     1081 acgattcggt cgcttcctcg aaccaatccg cgctcgggca ggaatcgtac tcagttggtc
     1141 taggactcat gtgaggctcc ttaaacgcga ggatactaca atacggcgta cttcttgaag
     1201 aaggcatcga gacgccctat tacggcttcc tgccttgggc ctacgggccg gggttctcgc
     1261 ttccgcggta gccagatatg cgtaaacacc cagtcgacgt tccgtgctca cgccgggcgg
     1321 aaccgccttt cgctcgacgc catcctgacg tctctcgctc caggccgcct ccgctcgctt
     1381 acggcgccaa ccatggcgcc tcttcgcgcc tttggcgcgc gctcgctacg gtacgtcgct
     1441 gcgcgccgca ttgtaaggta tcggattgaa cgtgcccgcc ggaagcgggc tgcactacga
     1501 ttccgcccgg ctccgagcct tgcctaggag tgcccgggcg ggatcgccct ccggccctcg
     1561 gcctcctgcc tcgggcctac gggccgcctc ggacgctcgc ggcgcatcca tgcgccgcgc
     1621 cctacgcttc gcttgggacg cgtcctcggt tcgattccgc ccggcgccct atataaaaag
     1681 acaaccaccc cgatggggtg gctttctttt taaggcgccg ggcggaatcg aaccgccgca
     1741 taaaggtttt gcagacctct cccttaccgc ttgggtacgg cgccgtattt gtagctgaat
     1801 gtcaaaagat ccagtgcgcc gtctagaacc ggtgccggat gaatatacct aaaacgttcg
     1861 atcgtgtaaa gcggtcggtg gttgctcgcc gaggccggcc atgaaggaga ccgtcgtgcc
     1921 cacgccgttc ctggaaccga tcatctccgc tgctgacata cagcttcttc gtccatcgtt
     1981 gcaggcggcc gcgaacgacc cctcgcatag gaaacgcacc cgg
//
LOCUS       DAOPHV010000522         2022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_79260_length_2022_cov_3.724453, whole genome shotgun sequence.
ACCESSION   DAOPHV010000522 DAOPHV010000000
VERSION     DAOPHV010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79260_length_2022_cov_3.724453"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PLQ29_11595"
     CDS             <1..632
                     /locus_tag="PLQ29_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG87330.1"
                     /translation="NLITGVYKVSSGTVSFGGQDITDLEPHRVAGLGVTRTFQNIRLF
                     KNLSVFENVLTACHASASYGLADSLLRLPRFRREEKAIKDKADGLLEILGLADRKDAI
                     ANGLPYGLQRRLEITRALALDPRLLLLDEPAAGMNPDETEQLMRLIGEIRDRFKLSVL
                     VIEHHMDLVMGLCEHIVVLNFGQKLADGAPDAIRGDKRVVEAYLGGGDQ"
     gene            634..1353
                     /locus_tag="PLQ29_11600"
     CDS             634..1353
                     /locus_tag="PLQ29_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG87331.1"
                     /translation="MLNVENLHVAYGGIRALKGVSLRVDEGEIVTIIGANGAGKSTLL
                     NAISGFLKPTSGYVAFRGKPLSRRPDLIVRDGICHVPEGRLVFANLSVESNLAMGAYL
                     RNDKKAVAADLERVYALFPRLAERAKQSAGTLSGGEQQMLAMGRALMTDGQLVLMDEP
                     SLGLAPLLVRTVFEIIGEIHKLGKTILLVEQNAFKALAVADRAYVLEQGRIVKEGKAS
                     EIAADPAVRDSYLGAESKAGG"
     gene            complement(1446..>2022)
                     /locus_tag="PLQ29_11605"
     CDS             complement(1446..>2022)
                     /locus_tag="PLQ29_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87332.1"
                     /translation="MSSTPDVFAESSPVIVVDLPEGYENYVATDVPFKYDGTSTVTPG
                     GNTFYTKKIVDSNNSIFQFSLSELYPKDASGNEMALEVFNFTGTEYKVYAPGIDGVPE
                     TYDILDYKDTPGMGILGNNIFTKVPWDGITILPETASVTFTLTWNLDGILELYDNKTS
                     DVTDDILVLADRFWERLSFSAVQYDINGNQL"
BASE COUNT          411 a          621 c          602 g          388 t
ORIGIN      
        1 tcaacctgat caccggcgtc tacaaggtca gctccggcac cgtctccttc ggcggccagg
       61 acatcaccga cctcgagcct caccgcgtcg ccgggctcgg cgtcacgcgt accttccaga
      121 acatcaggct gttcaagaac ctgagcgtct tcgagaacgt gctgaccgcc tgccacgcgt
      181 ccgcgagcta cgggctagcc gactcgctgc tgcggctgcc tcgcttcagg cgcgaggaga
      241 aggcgatcaa ggacaaggcc gacggactcc tcgagatact cggcctggcc gaccgcaagg
      301 acgccatcgc caacgggctg ccgtacggcc tgcagcgccg gctcgagata acccgggcgc
      361 tagcgctcga cccgaggctg ctcctcctgg acgagccggc ggccggcatg aaccccgacg
      421 agaccgagca gctcatgcgg ctcatcggcg agatccgcga ccgtttcaag ctgtcggtgc
      481 tggtcatcga acaccacatg gacctcgtca tgggcctctg cgagcacatc gtcgtgctca
      541 acttcgggca gaagctggcg gacggtgctc cggacgcgat acgcggcgac aagcgcgtcg
      601 tcgaggccta cctgggcgga ggagaccagt aacatgctca acgtagagaa tctccacgtc
      661 gcctacggcg gcatcagggc cctcaagggc gtatcgctcc gggtcgacga gggcgagata
      721 gtcacgataa tcggcgccaa cggggcgggc aagtcgacgc tcctgaacgc tatatccggc
      781 ttcctcaagc cgacctcggg ctacgtggcg ttcaggggca agcccctgtc gcgccgcccc
      841 gacctgatcg tgcgggacgg catctgccac gtgccagagg gccggctcgt attcgccaac
      901 ctgtcggtcg agagcaacct ggcgatgggc gcctacctgc gcaacgacaa gaaggccgtc
      961 gcggccgacc tcgagcgggt gtacgcgctg ttccctaggc tagccgagcg cgccaagcag
     1021 tcggccggca ccctgtcggg cggcgagcag cagatgctcg cgatgggccg cgccctgatg
     1081 accgacggcc agctcgtgct gatggacgag ccgtcgctcg ggctcgcgcc cctgctcgtg
     1141 cggaccgtct tcgagatcat cggggagatc cacaagctcg gcaagaccat cctgctcgtc
     1201 gagcagaacg ccttcaaggc gctcgcggta gccgaccggg cctacgtgct cgagcagggc
     1261 cgcatcgtca aggaaggcaa ggccagcgaa atagccgccg acccggccgt gcgcgactcc
     1321 tacctgggag cggagagcaa ggcggggggc tgatagtcgc tactagatga tatcagcaca
     1381 aggatgatcg gtaaatataa gggcgacctg aacgggtcgc ccttattcat ttttctgtac
     1441 aggatttata actgattgcc gtttatgtcg tactgtaccg cgctgaagga aagccgttcc
     1501 cagaaacggt cagccaatac gaggatatca tcggttacat cactcgtttt gttgtcatac
     1561 aactccagta tgccatcaag attccaggtt agtgtgaacg tcaccgaggc cgtttcgggt
     1621 agaatcgtta taccatccca tggtaccttg gtaaaaatgt tgttgcctaa tatgcccatt
     1681 cccggagtat ccttataatc gaggatgtcg tatgtctcgg gcacaccgtc tataccagga
     1741 gcatacactt tgtattctgt tccggtaaaa ttgaagactt ccaaagccat ctcattccct
     1801 gacgcatcct ttgggtacaa ctcgctcaag ctgaactgga aaatcgaatt attgctatcg
     1861 acaatctttt tcgtgtagaa ggtgttccct cccggggtga cggtcgaggt tccatcgtat
     1921 ttgaatggta catcggtcgc gacgtagttc tcatagcctt ccggtaaatc tactacgatg
     1981 acggggctgg attccgcgaa cacatcgggg gtggagctca tg
//
LOCUS       DAOPHV010000523         2022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_79265_length_2022_cov_3.465175, whole genome shotgun sequence.
ACCESSION   DAOPHV010000523 DAOPHV010000000
VERSION     DAOPHV010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79265_length_2022_cov_3.465175"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /gene="pheT"
                     /locus_tag="PLQ29_11610"
     CDS             <1..1081
                     /gene="pheT"
                     /locus_tag="PLQ29_11610"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPG87333.1"
                     /translation="ILMGADGGVLSMAPIINSADIGAVQVGDRDLLVEFTGTDMAAVC
                     LSASIVACDFSDAGYEIRPVRVDYGFDTPFGRSVTFPYYFQQPASVDSGRVSRLLGDS
                     FGPEQVVEALARMGVRAESHGQHVRATVPEYRDDFIHAVDLVEDVMIGRGLASFAPER
                     PRDFTIGRLSPIESFTRRAKDVMVGLGYQEMIYNYLGSGRDYIERMNVPADDVIRISN
                     PMTENFEYVRPSILPGLLQSEAVSSKAPYPHRIFEAGKVAFLDDARNYGTATRQRIGL
                     LCAHQAADYNELAGVVSALLYYLGEEYSVDDSDDPRFLPGRQARILIGGEPVGLFGEV
                     HPAVLENWGITMPCAACELDLESLL"
     gene            1098..>2022
                     /locus_tag="PLQ29_11615"
     CDS             1098..>2022
                     /locus_tag="PLQ29_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPG87334.1"
                     /translation="MDLEKRYSRLPDRDYPNFRAMFDSIFDRFGGLPAVKAKPEPGSP
                     YTVWSYADVRRESYAVGRFLLSKGLSAGDRVCVMAENRPEWCFAYIGIVAAGLVVVPV
                     DSTMDAAGLAGIIAASRSRALFYSPALGAKVAEALVQSPVDLAIEFPAVVGERKPAEK
                     PDAAASGAKERYGWLELGSPDDAGSGGAPEKSAERDARGLPAPAALDGDAPAAIIYTS
                     GTTGLAKGIVLSHRGVIANVNASIQSLNVDTRDVFIAVLPLHHTYAATCTFLSPMAAG
                     GDIVFIERIVPSVVLKHIREAGAMDAALSTKI"
BASE COUNT          324 a          703 c          641 g          354 t
ORIGIN      
        1 catcctgatg ggcgccgacg gcggcgtctt atcgatggct cccatcatca actctgcgga
       61 catcggcgcc gtccaggtgg gcgaccgcga cctgctcgtc gaattcaccg gcaccgatat
      121 ggccgcagtc tgcctctctg ccagcatcgt cgcctgcgac ttctccgacg ccggctacga
      181 gatacgtccg gtacgcgtcg actacgggtt cgatactccg ttcggccgct cggtcacctt
      241 cccgtactac ttccagcagc cggcctcggt agattcgggt cgcgtctcca ggctgctcgg
      301 cgacagcttc ggccccgagc aggtcgtcga ggcgctcgcc cgcatgggcg tccgcgccga
      361 gtcccatggg cagcatgtcc gggcgaccgt gcccgagtac cgcgacgact tcatccacgc
      421 ggtcgacctc gtcgaggacg tgatgatagg ccgcggtctg gccagcttcg ccccggagcg
      481 accgcgggac ttcaccatag gccgcctttc gccgatagag tcgttcacca ggcgcgcgaa
      541 ggacgtgatg gtcgggcttg gctaccagga gatgatctac aactacctcg gctccggcag
      601 ggactacatc gagcgcatga acgtaccggc cgacgacgtt atccggatct ccaacccgat
      661 gaccgagaat ttcgagtacg tacggccgtc tatcctgccg ggactcctgc agtccgaggc
      721 cgtgtcgagc aaggccccgt atccccaccg tatcttcgag gccggcaagg tcgccttcct
      781 cgatgacgcc aggaactacg gcacggccac gaggcagcgg atcggcctcc tgtgcgccca
      841 ccaggcggcc gactacaacg agttggcggg agtcgtctcg gccctgctct attacctcgg
      901 cgaggagtac tcggtcgacg actcggacga tccgaggttc cttcccggcc gtcaggcgcg
      961 tatactgata ggtggcgagc ccgtcggctt gttcggcgag gtgcatccgg ccgtcctcga
     1021 gaactggggc ataacgatgc cctgcgcggc ctgcgagctc gacctggaat cgctcctgta
     1081 acggccgggg aggtcgcatg gatctagaga aacgatactc caggctgcct gatcgggact
     1141 atccgaattt cagggcgatg ttcgactcga tcttcgaccg gttcggcggc ttgcctgcgg
     1201 tcaaggccaa acccgaaccc ggctcgccat atacggtatg gtcctacgcc gacgtccgcc
     1261 gcgaatcgta cgccgtcggg cggttcctcc tgtccaaggg cctgtccgcc ggcgatcgcg
     1321 tctgcgtgat ggcggagaac cggcccgagt ggtgtttcgc ctatatcggc atcgtggccg
     1381 ccggtctcgt cgtcgtgccg gtggactcga cgatggacgc tgccggcctc gccggcatca
     1441 tcgccgcgtc cagaagccgg gcgctgttct actcaccggc gctcggggcg aaggtggccg
     1501 aggcgctcgt ccagtcgccg gtcgatctcg cgatcgaatt ccccgcggtc gtcggcgagc
     1561 ggaagccggc cgagaagcca gacgccgccg cgtccggggc caaggaacgc tacggctggc
     1621 tcgagcttgg ctcgccggat gacgccggaa gcggcggcgc gcctgaaaaa agcgcggagc
     1681 gcgacgcccg agggctgccg gccccggccg ccctcgatgg tgacgcaccc gcggccatca
     1741 tctacacctc cggcacgaca ggcctggcca agggcatagt cctaagccac cgcggggtca
     1801 tagcgaacgt caacgcctcg atccagtcgc tcaacgtcga tacgcgggac gtcttcatcg
     1861 cggtgctgcc tctgcaccat acctacgccg ccacctgcac cttcctgtcg ccgatggccg
     1921 ccggcggcga catcgtgttc atcgagcgca tcgtcccgtc ggtcgtcctc aagcatatcc
     1981 gcgaggccgg cgccatggac gccgcgcttt cgaccaagat tc
//
LOCUS       DAOPHV010000524         2021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_79332_length_2021_cov_3.458291, whole genome shotgun sequence.
ACCESSION   DAOPHV010000524 DAOPHV010000000
VERSION     DAOPHV010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79332_length_2021_cov_3.458291"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLQ29_11620"
     CDS             <1..243
                     /locus_tag="PLQ29_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87335.1"
                     /translation="GEPVCVARRSWRELELFREPDDPVVAHALAFALEARRRAVSPER
                     RIAIATINGESAASSPYAGLLAGLGFEADRGVMTLW"
     gene            complement(340..>2021)
                     /locus_tag="PLQ29_11625"
     CDS             complement(340..>2021)
                     /locus_tag="PLQ29_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPG87336.1"
                     /translation="ASFSGALADVKEKFKQAPVAITIPMGEGADYKGVIDVLNQKAYP
                     TPASHDQKEAAVAIPAEYAAAVAEARAALSEAAAEGDDELMEKYLSEGELAQGEIVKG
                     LAEALAGNRVVPAFAGAATKNSGVAALIDFFVAIAPDPSKLSTEHAKTADGGETVMDV
                     DEKGPLAALVIKTQIDQFSGRLSYLKVVRGVLASDTEYLNVQENKKEKAGKLYTAQGK
                     KLEEVQELRAGDIGILAKSPSLKTNETISAPDAPVSFLPLRLPQPIHAIAISAVSKKE
                     EDKLSELLHKASEEDKTFTIRFDSETKETVIAGMGELHLNIILHKVKASQKIDVETRV
                     PRVAYRETITKHSGGEYTHKKQTGGHGQYARVVMEVEPLPRGEGYKFENKVFGGAVSK
                     GFIPGIEKGIHQAMEFGTIAGYPVVDVKSMLTDGKEHPVDSSEMAFKIAARGAFREAC
                     KAAGATLLEPIMRLAVFVDEKYLGDVMSDLSGRRGRISGQEPIGGGIVEIKAEVPQAE
                     LLRYSIDLKAMTSGTGGFEVEFDHYSPISGKIADDVAKAAAEFRIQEAEDE"
BASE COUNT          287 a          687 c          660 g          387 t
ORIGIN      
        1 ggcgagcccg tctgcgtggc caggcgatcg tggcgggagc tggaactgtt ccgcgagccg
       61 gacgaccccg tcgtcgcgca cgctctcgcc ttcgcgctcg aggccaggcg ccgggcagta
      121 tcgccggagc gccgcatcgc catcgccacg atcaacggcg agagcgccgc ctcgtcgccc
      181 tacgccggcc tgctcgccgg gctcggcttc gaggccgacc gcggcgtcat gacgctctgg
      241 tagccctgaa cggcgccgac ggacgcacac atccgcgggg cgtaaaaaaa gggaccgtcc
      301 cgcaggacgg ccccatggct cgctagcgcg gccgggcaat cattcgtcct cggcttcctg
      361 gatcctgaac tccgcggcgg ccttggccac gtcatcggcg atcttgccgg agatcggcga
      421 gtaatggtcg aattccacct cgaagccgcc ggtgccgctg gtcatggcct tgaggtcgat
      481 cgaatagcgc aggagctcgg cctgcggcac ctcggccttg atctcgacga tgccgccgcc
      541 tatcggctcc tggccggaga tgcggccgcg gcggcccgac aggtcggaca tcacgtcgcc
      601 gaggtatttc tcgtcgacga agacggccag cctcatgatc ggctcgagca gggtggcgcc
      661 ggcggccttg caggcctcgc ggaacgcgcc gcgcgcggcg atcttgaacg ccatctcgga
      721 cgagtcgacc gggtgctcct tgccgtccgt caacatgctc ttaacgtcga cgaccgggta
      781 accggctatc gtgccgaact ccatcgcctg gtggatgccc ttctcgatgc cggggatgaa
      841 acccttggac accgcgccgc cgaagacctt gttctcgaac ttgtagccct cgccgcgcgg
      901 caggggctcc acctccatca ccacgcgggc gtattggccg tgtccgccgg tctgcttctt
      961 gtgggtatac tcgccgcccg agtgcttggt gatcgtctcg cggtacgcta cgcgcggcac
     1021 gcgggtctcc acgtcgatct tctgcgaggc cttgacctta tggaggatga tattcaggtg
     1081 cagctcgccc atgccggcga tcaccgtctc cttggtctcc gagtcgaagc gtatcgtgaa
     1141 ggttttatcc tcctccgaag ccttgtggag tagctccgac agcttatcct cttccttctt
     1201 ggacacggcg ctgatcgcga tggcgtggat aggctggggc agccggagcg gcaggaacga
     1261 caccggcgcg tctggcgcgg agatggtctc gttcgtcttg agcgacgggc tcttggccag
     1321 gatgccgatg tcgccggcgc gcagttcctg gacctcctcg agcttcttgc cctgggcggt
     1381 atagagcttg cccgccttct ccttcttgtt ctcctgcacg ttcaggtact cggtatcgga
     1441 ggctagcacg ccgcgtacca ccttcaggta cgatagcctt ccggagaact ggtcgatctg
     1501 ggtcttgatc acgagtgcgg ccaagggtcc cttctcgtcg acgtccatga cggtctcgcc
     1561 gccgtcggcc gtcttggcat gctccgtcga cagcttggac gggtcgggcg cgatggcgac
     1621 gaaaaagtcg atcagagccg cgacgccgga gttcttggtg gcggcgccgg cgaaggccgg
     1681 cacgacgcgg ttgcccgcca gggcctcggc caggcccttg acgatctcgc cctgagccag
     1741 ctcgccctcg gacaggtatt tctccatcag ctcgtcatcg ccctcggccg ccgcctcgga
     1801 cagggcggcg cgcgcttcgg ccacggccgc ggcgtactcg gcggggatcg cgaccgccgc
     1861 ctccttctgg tcgtgcgacg ccggcgtcgg ataggccttc tggttgagca cgtcgatgac
     1921 gcccttgtag tcggcgccct cgcccatcgg tatggttatg gccacggggg cctgcttaaa
     1981 tttctctttc acgtcggcta aggcgccgga gaacgaggcc c
//
LOCUS       DAOPHV010000525         2020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_79378_length_2020_cov_3.907888, whole genome shotgun sequence.
ACCESSION   DAOPHV010000525 DAOPHV010000000
VERSION     DAOPHV010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79378_length_2020_cov_3.907888"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PLQ29_11630"
     CDS             complement(<1..772)
                     /locus_tag="PLQ29_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPG87337.1"
                     /translation="MATRTVIEIDEDKCDGCGLCASGCHEGALRIIDGKARLVGESLC
                     DGLGACIGECPRGALKTVERDVEEYDERRVIEHILPKGMATIAAHLDHLSSHGQDGWY
                     AQALEVLAEKGIAAPKTAVEAEAGGCGRGVSSAAGANRLPRFSLEPIPTDAPTPRAVD
                     NSMSGGCPGSAARSFRSAAAGRASASGSVEAPTAGSMASRLEHWPVQLHLVNPRAPHF
                     AGAHLLIAADCTAFACGAFHQALLAGRKLVIACPKLDTG"
     gene            complement(925..1401)
                     /locus_tag="PLQ29_11635"
     CDS             complement(925..1401)
                     /locus_tag="PLQ29_11635"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPG87338.1"
                     /translation="MPANGIIHLSDASLIAIHALVGLADAEGRLVQGRDLAAIIDASE
                     NHLAKVMQRLVRASIVRSVKGPNGGFALDKRPEDISFKEAIEAVDGPISSGFCPFRTD
                     RCNPNNCIFGKEISKHAFELVAYLSQRTVADIARERAPDSPPAYHAVIGNRTKRGA"
     gene            1585..>2020
                     /gene="aroQ"
                     /locus_tag="PLQ29_11640"
     CDS             1585..>2020
                     /gene="aroQ"
                     /locus_tag="PLQ29_11640"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPG87339.1"
                     /translation="MLISVINGPNLNMLGEREPQVYGSETLDDINSALGRAARDRGVD
                     VEFFQSNSEGSLIDRLHECSRRSDGVILNAGAYTHYSIAIRDAIAAIRPPVVEVHLSN
                     VHAREEFRRVSVIAPACVGQISGFGSTSYLLALEALVRIYEER"
BASE COUNT          353 a          616 c          639 g          412 t
ORIGIN      
        1 taccggtatc gagcttgggg caggctatta ctagtttcct acccgccaga agggcttggt
       61 ggaaggctcc gcaggcgaag gcggtgcagt ccgccgctat gagcagatgg gccccggcga
      121 aatgcggggc gcgcgggttg acgaggtgta gctgtaccgg ccaatgctcc agtctcgagg
      181 ccatgctccc tgcggtcggg gcctcgaccg atccggaggc ggacgcccgt ccggccgcgg
      241 cggatcggaa ggagcgcgcg gccgagcccg ggcagccccc ggacatgcta ttgtcgaccg
      301 cgcgcggggt cggtgcgtcg gtcggtatcg gttcgagcga gaaacggggg agccggttgg
      361 cgccagcggc tgacgagacg cctcggccac agccgccggc ctcggcctcg acggcggtct
      421 tgggcgcggc tatgcccttc tccgctagga cctcgagggc ctgggcatac cagccgtcct
      481 gtccatggct ggaaaggtgg tcgaggtggg cggctatcgt ggccatgccc ttgggcagga
      541 tgtgctcgat gacgcggcgt tcgtcgtatt cctcaacgtc ccgttcgacg gtcttgaggg
      601 cgccgcgcgg gcattcgccg atgcaggcgc cgagtccgtc acataggctt tcgccaacga
      661 gtctggcctt gccatcgatg atcctgagcg cgccttcgtg gcagccgctt gcgcatagtc
      721 cgcagccatc gcacttatcc tcgtctatct ctatgaccgt tcgtgtagcc atgatctcct
      781 cctcgtctcg atgttgactt atgtcgtgct tgcattcata agggcaaaga ggaactataa
      841 aaccgaaaaa ccgatattta aatcctctcc taccatacta tctgttattg tcctaactcg
      901 ccgtcagccg cggtcagcca tgattcacgc cccgcgttta gtcctattgc cgatgaccgc
      961 atgataggcc ggcggcgagt cgggcgcgcg ttcgcgcgcg atgtccgcca ccgtccgctg
     1021 gctcaggtag gccacgagct cgaaggcgtg tttggatatt tctttgccga agatacagtt
     1081 gttcggattg caacggtcgg ttcggaacgg gcagaacccg ctcgagatag gaccgtcgac
     1141 ggcctcgatc gcctccttga acgagatatc ctcggggcgc ttatcaagag cgaagccgcc
     1201 gttcgggccc ttgaccgagc gcacgatact ggcgcggacg agtcgttgca ttactttggc
     1261 aaggtggttc tcgctcgcgt cgatgatggc cgcgaggtcg cggccttgga ccagccgacc
     1321 ctcggcgtcg gcgaggccga cgagcgcgtg aatcgcgatg aggctggcgt ctgaaagatg
     1381 gatgatcccg ttcgcaggca ttgtggtatt ttaataccac aattaatatc cactgtcaag
     1441 cgcttgtatc ccgattcggt cgaactgtat cggctagccc accacaagga ggtgcctgcg
     1501 gtcgcgacgc tccagtccat agtttgacag cagcgtctcg cgcgtataga gttgtcccgc
     1561 ggtggaggcc ggagcgtgaa aacgatgctg atttccgtca tcaacgggcc taatttgaac
     1621 atgctcggcg agcgcgaacc acaggtgtac ggttccgaga cgctcgacga catcaatagc
     1681 gccttgggcc gggcggcccg cgatcgcggt gtcgacgtcg aatttttcca gtccaattcc
     1741 gagggctcgc ttatagaccg cctgcatgaa tgctcccggc gctccgacgg agtcattctc
     1801 aacgccggcg cgtacaccca ctactcgata gccatccgag acgctatcgc cgccatcagg
     1861 cccccggtcg tcgaggtaca cctctccaat gtccacgccc gcgaggaatt caggcgggtt
     1921 tcggtcatag cgccggcatg cgtcggccag atatcaggct tcggttcgac gagctacctg
     1981 ctggcgctcg aggcgctggt ccgtatatac gaggagcgat
//
LOCUS       DAOPHV010000526         2019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_79453_length_2019_cov_3.526477, whole genome shotgun sequence.
ACCESSION   DAOPHV010000526 DAOPHV010000000
VERSION     DAOPHV010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79453_length_2019_cov_3.526477"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PLQ29_11645"
     CDS             complement(<1..721)
                     /locus_tag="PLQ29_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG87340.1"
                     /translation="MASAAQMVALLRSHADGDDEHFYAVALQVAAQEAKQGHERLASE
                     IKQLVDEAKLTRARRTVVPLNKHTGELANLLTVSYPSSRLGDIVLGARLADQLKRVIK
                     EHRNAGRILEHGLSPRRKLMLVGPPGTGKTFSASVLAGELGLPLFVVRLDGLLTKYMG
                     ETAVKLRQIFDATDRTRGVYFFDEFDAIGSERGLSNDVGEVRRILNSFLQMIDHDPSN
                     SLIMAATNHPEILDRALYRRFD"
     gene            complement(797..1462)
                     /locus_tag="PLQ29_11650"
     CDS             complement(797..1462)
                     /locus_tag="PLQ29_11650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HPG87341.1"
                     /translation="MKNVRLSVYPPEGSVKMTVPLFTGDGAIHSAIVSRLAWIRRQQE
                     RFRTQARQTKREMVNGETIYWLGRRYRLNVVESPRASYVLQERIGYIDLYCRKDAGAE
                     RRESILYEWYRSRLKMVVPGLLATWEPVIGVHAEDWGIKRMKTRWGTCSPAAKRIWLN
                     LELAKKSPHCIEYVLVHELVHLLEPSHNARFTALMTRFMPLWTHYRSELNQAPLADED
                     WKY"
     gene            complement(1521..>2019)
                     /locus_tag="PLQ29_11655"
     CDS             complement(1521..>2019)
                     /locus_tag="PLQ29_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87342.1"
                     /translation="LIVERGPAALDELPEGLRGNGEAIAETIENNVRRTIVDQEAINP
                     KYYELMSALLDELIKKRREEALSYKEYLDEIVMLAKKITKPGGGSRYPPDITTQGQRA
                     LYDNLGDHDLALAVHEGVLREAQHGWREIVIRRRKLRQWLATMFTDEAELDRILGIIE
                     ANHEY"
BASE COUNT          315 a          591 c          668 g          445 t
ORIGIN      
        1 cgtcgaatcg cctatagagc gcccggtcta gtatctcagg gtggttagta gcggccatga
       61 tgaggctgtt cgacggatca tggtctatca tctgcaggaa gctgttgagg atacgcctga
      121 cctcgccgac gtcgttcgaa aggccgcgtt ccgagccgat ggcgtcgaac tcgtcgaaga
      181 agtagacgcc cctcgtcctg tcggtggcgt cgaatatctg cctgagcttt accgcggtct
      241 cgcccatgta cttggtgagc aggccgtcga gccggacaac gaagagcggc aggccgagct
      301 ctccggccag caccgacgcg gagaaggtct taccggtacc gggagggccc acgagcatca
      361 gcttgcggcg gggcgatagg ccgtgctcca gaatcctgcc ggcgttgcgg tgctccttga
      421 tgacgcgctt gagctgatcc gccagcctcg cgccgagcac aatgtcgccg aggcggctgg
      481 acggatagct cacggtcagg agattggcga gctctcccgt atgcttgttc agggggacga
      541 cggtgcgcct ggccctagtc agcttggcct cgtctaccag ctgcttgatc tcggaggcga
      601 gccgctcgtg cccctgctta gcctcctggg cggccacttg cagggctacg gcatagaaat
      661 gctcgtcgtc gccgtccgcg tgagaccgta ggagtgcaac catctgggcc gcgctggcca
      721 tccaaggctc cttgagctag aagatactac ggtataccac gttttacggt tcttgcttag
      781 tcccggacag ccctagtcaa tacttccaat cctcgtcggc caggggggct tgattcagtt
      841 cggaacggta gtgcgtccag aggggcatga accgggtcat gagggcggtg aagcgggcgt
      901 tgtggctcgg ctccaggagg tggacgagtt catggacgag gacgtactcg atgcagtggg
      961 gggatttctt ggccagttcg aggttgagcc agatccgttt ggccgcgggg ctgcaggtgc
     1021 cccagcgggt cttcatgcgc ttgatgcccc agtcctcggc gtggacgcct atcaccggct
     1081 cccaggtggc gaggagtccg ggtaccacca tcttgagccg gcttcggtac cattcgtaca
     1141 ggatgctttc gcgccgttcg gcgccggcgt ctttcctgca gtagagatcg atgtagccta
     1201 tcctctcctg cagtacgtag gacgcccgcg ggctttccac cacgttgagc ctgtagcgcc
     1261 ggcccagcca gtagatggtc tcgccgttga ccatctcgcg cttggtctgt cgggcctggg
     1321 tcctgaagcg ttcctgctgt ctgcgaatcc aggcgagccg ggagacgatg gcgctatgga
     1381 tggcgccatc gccggtaaag agcggcaccg tcatcttcac ggagccttcc gggggataga
     1441 cggagaggcg gacgttcttc acggcctttc gggtgacgag tacggtgatt tcctggacct
     1501 gtacggcgaa ggccttggct ttagtactcg tgattcgcct cgatgattcc gagtatccgg
     1561 tcgagctccg cctcgtcggt gaacatggtc gccagccatt gccgcagttt gcgcctgcgt
     1621 atgacgatct cgcgccagcc gtgctgggct tcgcggagta cgccttcgtg cacggccagg
     1681 gccaggtcgt ggtcgcccag gttgtcgtag agggcccgct ggccctgggt cgttatgtcc
     1741 gggggatagc gcgatccgcc gccgggcttg gttatctttt tggcgagcat gactatctcg
     1801 tcgaggtatt ccttatacga cagggcttct tcccgccgct tctttatcag ttcatcgagg
     1861 agggcgctca tcagttcgta gtacttgggg ttgatggctt cctggtcgac gatggtgcgg
     1921 cggacgttgt tctcaatggt ctcggctatg gcttcgccgt tgccgcggag accttcgggc
     1981 agttcgtcca gcgcggcggg gccccgctcg actatgagc
//
LOCUS       DAOPHV010000527         2010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_79928_length_2010_cov_3.857289, whole genome shotgun sequence.
ACCESSION   DAOPHV010000527 DAOPHV010000000
VERSION     DAOPHV010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79928_length_2010_cov_3.857289"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..360
                     /locus_tag="PLQ29_11660"
                     /pseudo
     CDS             76..360
                     /locus_tag="PLQ29_11660"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
     gene            536..877
                     /locus_tag="PLQ29_11665"
     CDS             536..877
                     /locus_tag="PLQ29_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87343.1"
                     /translation="MENGVSAASKVIISIIPIVGIVMGCTVVFFYILWSHREKMLMIE
                     KGSYAPKPLDVDVFSLLAGILLVAVGITLTAVFLVMDTAGYTLLGGLIPLSVGLGFLS
                     FYSLRARRRAT"
     gene            874..1431
                     /locus_tag="PLQ29_11670"
     CDS             874..1431
                     /locus_tag="PLQ29_11670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPG87344.1"
                     /translation="MTPSGSEAADEDIIARVLDGDADSFGALVRRYQRRILRLGYGFF
                     KDADEAEDFAQDVFVKAYVGLAGFKGRSSFSTWLTRIAYNAGINAKRKAGRYEPLDSE
                     PVDSRTLSPEDAHIRAETAEALRAAMAALPEKYAVCLDLYFREGMRYEDISASTGFPV
                     NTIKSHVFRAKRDLRKALGAEEGPL"
     gene            1428..1889
                     /locus_tag="PLQ29_11675"
     CDS             1428..1889
                     /locus_tag="PLQ29_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87345.1"
                     /translation="MNCRSFRNQLDALDNDDLPIEMLVHARSCPECAAEAAALKAALS
                     LYRLPDLVGSPYVAPRVMALLPFVPAPRRSVSMGNWLVTGFVIVFSMVLVPLLAEFRA
                     LKAVYGNVFTVSLSMALGLFVTLYAGAFIMSHLDEFTRRLKEREAQHGRAA"
BASE COUNT          328 a          667 c          651 g          364 t
ORIGIN      
        1 ggttgccggt gacggccagg ccgccgtcgg actgcctgtc gacgctcagg tggcagccgc
       61 tgccctcgcg gcgtcaagta cgccaacgag gagttgctcg cgcccaggcg cgtggtcagc
      121 gcgaccgcgg ccgtcgaggg cggcgccgcc tgctccggca acccgggcgg cttgtgcgcc
      181 gtctcacgcc tgccggtccg ctcgagcgcc gcctacccga agcagggcgt gcccgagctg
      241 ctcgaggcca tctacgcgct acgcgtcagc ctgccggtca gccgcggcga cgtactgatc
      301 aaggacttca agggctcagg cgtcgacgtc atagcgacga gaacccttaa gaaggcctga
      361 tagcggctcg gccgccgcct cccgcggccg cccagggccg catcggaata ccgctcgaca
      421 gagcccgccg acttcggatc gaagatccgc aaccggcggg ctattttatg gtctatatgt
      481 cagtgccccg ggagtacgcg ggcccgatag tgtatacttg cgatacggag cgcttatgga
      541 aaacggagtg tcggcggcgt ccaaggtgat catctcgatc atccccatcg tgggtatcgt
      601 gatgggttgc accgtcgtgt tcttttacat cctatggagc catagggaga agatgttgat
      661 gatcgagaag ggcagttacg cgcccaagcc gctcgacgtc gacgtgttca gcctgctggc
      721 ggggatactc ctcgtggcag tcgggataac cctgacggcg gtcttcctgg tcatggacac
      781 cgccggctac accctgctcg ggggactcat acccctttcg gtcggcctcg gctttctgtc
      841 cttctactcg ctacgcgccc gccgacgggc gacatgacgc cgagcggatc ggaagccgcc
      901 gacgaggata tcatcgccag agtgctcgac ggggacgcgg actcgttcgg cgcgctggtg
      961 cgcaggtacc agcggagaat actgcgcctc gggtacggct tcttcaagga cgccgacgag
     1021 gccgaggact tcgcccagga cgtgttcgtc aaggcttacg tcggcctggc cggctttaag
     1081 gggcgctcca gcttctcgac ctggctgacg cgcatcgcct acaacgccgg catcaacgcc
     1141 aagcgcaagg ccgggcgcta cgagccattg gactcggagc cggtggattc gaggacgcta
     1201 tctcccgagg atgcccacat ccgcgccgag accgcggagg cgctcagggc cgccatggcc
     1261 gccctgccgg agaaatatgc cgtctgcctg gacctgtact tcagggaggg tatgcggtac
     1321 gaggatataa gcgcgtcgac cggctttccg gtcaacacga tcaagtcgca cgtgttcagg
     1381 gccaagcggg acttgagaaa ggcgctcggc gccgaggagg ggccgctatg aattgcagga
     1441 gcttcaggaa ccagctcgac gcgctcgata acgatgacct gccgatcgag atgctcgtcc
     1501 acgcgcgttc gtgccccgaa tgcgcggccg aggccgccgc cctcaaggcg gcgctgagcc
     1561 tgtaccgcct gcccgacctg gtcggctccc cctatgtcgc gcccagggtc atggccctct
     1621 tgcccttcgt gccggccccg cgccgctcgg tgtccatggg gaactggctg gtgacaggct
     1681 tcgtcatcgt tttctcgatg gtgctcgtgc ctctcctcgc cgagttccgt gcgctcaagg
     1741 ccgtctacgg caacgtcttc accgtgtctt tgtcgatggc gctcggcctc ttcgtcaccc
     1801 tctatgccgg cgccttcatc atgagccacc tggacgagtt cacccggcgc ctcaaggagc
     1861 gcgaggccca gcacggacgg gctgcctaga ggcgcggggt agccctgatt atctgatatt
     1921 ccgtagccag gtacaggcgc ctcgccgagt cgacggctat ccccctggcg cataggtcga
     1981 ccgtccgtcc gtccaaatgg aagcgtagct
//
LOCUS       DAOPHV010000528         2001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_80446_length_2001_cov_3.013875, whole genome shotgun sequence.
ACCESSION   DAOPHV010000528 DAOPHV010000000
VERSION     DAOPHV010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80446_length_2001_cov_3.013875"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..1428
                     /locus_tag="PLQ29_11680"
     CDS             106..1428
                     /locus_tag="PLQ29_11680"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPG87346.1"
                     /translation="MRSRINSDKFNILVYFVSVIAVGTMLLSSPPAWAGEDRLGLLDS
                     LFTATSAVCVTGLAVVDTAGFSLFGQIVLLLLIQCGGLGIVAFATLYVAIPRRKVSLV
                     NRGIIKEMYMDEVESNPRYIVLNIAAVTLAIETIGFCILLARFRSIGVDGAAFSAAFH
                     AVSAFCNAGFSTFSDSLIGFAGDWVVNLVVMALIVSGGIGFVVMQDIGRVFMRSKRRL
                     SYHSRIVLSITATLIVFGAALYYALEYNSAFSGMGLGDGLLAALFESVTTRTAGFETV
                     PQGSFGYASVILTIFLMLTGGSPGSTAGGVKTTTMFMAFAAAFRGTEEDGSISYRGGA
                     LSTTSVTRAFSILAKALLIIGACLFVLLAFEGSRFAFVDLLFEIVSAFGTVGLSRGVT
                     PGLGAWGKLVIIAAMFIGRIGVFAMAVSRPSDRVERFAAYPRENLLLG"
     gene            1438..>2001
                     /locus_tag="PLQ29_11685"
     CDS             1438..>2001
                     /locus_tag="PLQ29_11685"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPG87347.1"
                     /translation="MARQFALVGLGSFGLSVLEKLAEATDQIIILDKEKSVIEKYKDL
                     AAKSYISDALNEEALRRIVPEGIDVAIVDVGDHLEAAIIVTNALKKLGAKQIIVRADS
                     EDRGEILEMVGATRIIYPAKEAAYRIVPMLVSSSLFSFMPISASMVMAEFRVPGRYVG
                     MTLIEANLRQTKGINVVALRKTDEDDYV"
BASE COUNT          353 a          574 c          638 g          436 t
ORIGIN      
        1 aaggtgcatt gaagactcga tagggtcacg cgacgggagg gcgcggggcg ttcggatatg
       61 gcgcgtcttg aaggtagcta tctggacgtg gtaccatcca gggggatgag atccaggatc
      121 aatagcgata agttcaacat acttgtttac ttcgtctcgg tgatagccgt cgggacgatg
      181 ctattatcgt cgccgccagc ctgggctggc gaggacaggc tcgggttgct cgattctctc
      241 tttaccgcta cgagtgccgt ctgcgtgact gggctcgctg tcgtcgatac ggccgggttc
      301 agcctgtttg gccagatagt cctgctccta cttatacaat gcggcggcct tggtatcgtc
      361 gccttcgcca ccctctacgt ggcgatcccg cgccgtaagg tatctttggt caaccggggc
      421 atcatcaagg aaatgtacat ggacgaggtc gaatcaaacc ctcgctatat cgtactcaac
      481 atagctgcgg ttaccctcgc catcgagacg ataggcttct gtatcctctt ggcgcggttc
      541 cggtcgatcg gcgtcgacgg ggccgcattc tcggccgcgt tccatgccgt ctcggccttc
      601 tgcaacgccg gcttctcgac cttcagcgac agcctgattg ggttcgccgg ggattgggtc
      661 gtcaacctcg tcgtgatggc gctgatcgtt tcgggcggta tcggcttcgt cgttatgcag
      721 gacatcgggc gcgtgttcat gcgctccaag cgtcggctca gctaccattc gcgcatcgtg
      781 ctgtcaataa cggcgacgct gatcgtgttc ggggctgcac tgtactatgc gctggagtac
      841 aacagcgcct tctccggcat gggcttgggc gatgggctgc tcgccgcgct gttcgagtcg
      901 gttacgacga ggacagccgg cttcgagact gtgcctcagg gctcctttgg gtacgcgtcc
      961 gtcatcctga ccattttcct gatgctcacg ggagggtctc ccgggtcgac ggccggcggc
     1021 gtcaagacga ccacgatgtt catggccttc gcagccgcct tccgcggcac ggaggaagac
     1081 gggtccatct cgtatcgagg cggagccctg tcgacgacat cggtgacgcg ggcgttctcg
     1141 atcctcgcca aggcgctgct gatcatcgga gcgtgtctgt tcgtgctcct cgcgttcgaa
     1201 ggtagcaggt tcgccttcgt cgaccttctg ttcgagatag tatcggcttt cggaaccgtg
     1261 ggcctgagcc gcggggtcac gcccgggcta ggtgcctggg gcaagctcgt catcatcgcg
     1321 gctatgttca taggcaggat tggcgtgttc gccatggcgg tatcgcgccc gtccgacagg
     1381 gtcgagcgtt tcgccgcgta tccgcgagag aacctgctgc tgggatagga gggaacgatg
     1441 gcaaggcaat tcgctctcgt cggcttgggt agcttcgggc tgagcgtcct cgagaagctc
     1501 gccgaggcta ccgatcagat catcatactg gacaaggaaa agtcggtaat cgagaagtac
     1561 aaggatctgg cggcaaagtc gtacatcagc gacgcgctga acgaggaggc cttgcgccgt
     1621 atcgtcccgg aggggatcga cgtggcgatc gtcgacgtcg gcgaccacct cgaggcggcg
     1681 atcatcgtca ccaacgccct caagaagctg ggggccaagc agatcatcgt gcgggccgac
     1741 agcgaggacc gtggcgagat actcgagatg gtcggcgcga ccaggatcat ctaccccgcc
     1801 aaggaagcgg catatcgtat cgtaccgatg ctcgtgtcca gttcgctttt ctccttcatg
     1861 ccgatcagcg cgagcatggt gatggccgag ttccgggtac ccggtcgcta tgtcggcatg
     1921 acccttatcg aggccaacct gaggcagacc aagggtatca acgtcgtcgc tctacgcaag
     1981 acggatgagg atgactacgt a
//
LOCUS       DAOPHV010000529         1995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_80786_length_1995_cov_3.923196, whole genome shotgun sequence.
ACCESSION   DAOPHV010000529 DAOPHV010000000
VERSION     DAOPHV010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80786_length_1995_cov_3.923196"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="PLQ29_11690"
     CDS             <1..921
                     /locus_tag="PLQ29_11690"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPG87348.1"
                     /translation="DLGLAGKRSGKVRDWYPLPGGRRLIVTTDRLSAFDRTLAAVPYK
                     GQVLNQLSALWFERTRDIVGNHLLSVPDPNAVVALEAEPLPIEVIVRGYITGVTSTAL
                     WYRYSLGERSIYGYDFPDGLRKNQALPEPIITPTTKGGPTGHDERLTGVEVVSGGYLD
                     EADWATVSRAALALFRRGQETAAAAGLLLVDTKYEFGRAPDGSIILIDEVHTPDSSRF
                     WKAESYRSRFEAGHEPECFDKEYVRLAFAELGYRGDGPVPPVRDSLWVETSSLYRSAY
                     ERLTGESFVPGESPVGPRLERNLRAAGLLS"
     gene            918..1430
                     /locus_tag="PLQ29_11695"
     CDS             918..1430
                     /locus_tag="PLQ29_11695"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPG87349.1"
                     /translation="MSGAMVVILMGSKSDEAHCATIAEALDALGVAHESRVGSAHKTP
                     ARVLAMLAEYEASDRPRVYVTVAGRSNALSGFVDGAVEAPVLACPPPPDAFGGADLYS
                     SIRMPGGIAPALVLDPANAALAAAKILGLSDPAIAARVRAFREANADAIATADAIAAT
                     RGDGTEGRLA"
     gene            1463..>1995
                     /locus_tag="PLQ29_11700"
     CDS             1463..>1995
                     /locus_tag="PLQ29_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HPG87350.1"
                     /translation="MGSDASIRRDKAHDECGVVAINNGVAINNGVVLASGTTSAGGSD
                     AARAAFYALFSLQHRGQESAGLASADGRGVRIRKGLGLVSRAFSEADVEELTGPLAIG
                     HTRYSTTGEPSLRNAQPFYVETMHGPLALAHNGNLVNAAELRRAMLERGVGLVSSSDT
                     EVMVMMLAAARGDDWPER"
BASE COUNT          302 a          695 c          704 g          294 t
ORIGIN      
        1 gatctagggt tggctggcaa gcgctcgggc aaggtgaggg actggtaccc gctaccgggc
       61 gggcggcggc ttatcgtcac caccgaccga ctttcggcgt tcgaccggac gctggcggcg
      121 gtaccgtaca aagggcaagt gctcaaccag ctgtcggcct tgtggttcga gcggacgagg
      181 gacatcgtcg gcaaccacct gctgtcggtc cctgacccga acgccgtcgt ggcgctcgag
      241 gccgagccgc tgcctatcga ggtgatcgtg cgcggctaca tcaccggggt cacctcgacc
      301 gccctctggt accgctactc gctcggcgag cgatcgatct acggctacga tttcccggac
      361 ggcctgcgca agaaccaggc cctgcccgag cccatcatca cgccgacgac gaagggcggg
      421 ccgaccgggc acgacgagcg gctgaccggg gtcgaggtgg tctccggagg ctacctcgac
      481 gaggcggact gggcgacggt gagccgcgcg gccctggcgc tgttcaggcg cggccaggag
      541 acggcagcgg ccgcggggct cctgctcgtc gacacgaagt acgagttcgg ccgcgccccg
      601 gacggctcta tcatactgat agacgaggtg catacgcccg actcgtcgcg cttctggaag
      661 gccgagagct accgctcccg attcgaggcc gggcacgagc cggaatgctt cgacaaggaa
      721 tacgtgcggc tggcgttcgc ggagctgggc taccgcggcg acggcccggt accgcccgtg
      781 cgcgactcgc tctgggtcga aacgagctcg ctgtaccgct cggcctacga gcgcttgacc
      841 ggcgagtcct tcgtgccggg agaatccccg gtcgggccga ggctcgagcg caacctgcgc
      901 gccgcggggc tgctatcgtg agcggcgcca tggtcgtcat cctgatgggc tccaagtccg
      961 acgaagcgca ctgcgctacg atagccgaag ccctcgacgc gctcggcgtg gcgcacgaga
     1021 gccgggtcgg ctcggcccac aagacgccag cgcgcgtgct ggccatgcta gccgagtacg
     1081 aggcctcgga ccggccgcgc gtctatgtaa cggtagccgg taggtccaac gcgctgtccg
     1141 gcttcgtcga cggcgcggtg gaggcgccgg tgctggcctg cccgccgccg ccagacgctt
     1201 tcggcggcgc cgacctctac tcgtcgatac ggatgcccgg cggcatcgcg ccggccctgg
     1261 tgctggaccc ggcgaacgcg gcgctggccg cggccaagat cctgggcctg tcggatccgg
     1321 ctatcgcggc ccgggtcagg gccttccgcg aggccaacgc cgacgccatc gcgaccgccg
     1381 acgccatcgc ggcaacgcgc ggagacggca cggaagggcg gctagcgtga tcggcgactc
     1441 gtccatccga cacgatgcgc ccatgggaag cgacgcgtct attcgacgcg acaaagcgca
     1501 tgacgagtgc ggcgtcgtcg ccatcaataa tggcgtcgcc atcaataatg gcgtcgttct
     1561 tgctagcggt acgacctccg ccggcggctc cgacgcggcg cgcgcggcct tttacgcgct
     1621 attctcgctc cagcaccggg gtcaggagtc cgcgggactc gcttcggccg acggccgcgg
     1681 ggtacggatc cgcaagggcc tcggcctcgt ctcgcgcgcc ttctccgagg ccgacgtcga
     1741 ggagctgacc gggccgctgg ccatcggtca tacccggtac tcgacgaccg gcgagccgag
     1801 cctccgcaac gcccagccgt tctacgtgga gacgatgcac ggcccgctcg cgctcgcgca
     1861 taacggcaac ctggtcaacg ccgccgagct caggcgcgcc atgctcgagc ggggcgtcgg
     1921 gctcgtctcg tcgtccgaca ccgaggtgat ggtgatgatg ctcgccgcgg cccgcggcga
     1981 cgactggccg gagcg
//
LOCUS       DAOPHV010000530         1994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_80844_length_1994_cov_4.984528, whole genome shotgun sequence.
ACCESSION   DAOPHV010000530 DAOPHV010000000
VERSION     DAOPHV010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80844_length_1994_cov_4.984528"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..993
                     /locus_tag="PLQ29_11705"
     CDS             277..993
                     /locus_tag="PLQ29_11705"
                     /inference="COORDINATES: protein motif:HMM:NF018604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HPG87351.1"
                     /translation="MHRLPAWVGLGSGVLAFTAGTMNVFTLESALHQAVTHHSGSSSS
                     MMLALSLGHYGLALRFLAIILAFVAGSVLSGFIIRDYHLRLGRRYGVSLMIESACILV
                     AWSLASERPFATQLLLAGASGLQNAMATTYSGAVVRTTHLTGIYTDIGVIVGNRLAGI
                     RSQPGKLRLLAGILASFLLGGLANSLLFPYLGNGVLAIPAAITGTLGFGYWVYRQLYL
                     KPRGQVADTPAGDGAAPGAP"
     gene            complement(1005..1430)
                     /locus_tag="PLQ29_11710"
     CDS             complement(1005..1430)
                     /locus_tag="PLQ29_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HPG87352.1"
                     /translation="MRKTIVVVDGMGGGLGAQLIAKTRELIGDAAEIVALGANSCATE
                     RMLKAGADRGASGENAVRVSVGLGDVVLGPIGVIAPNAMMGEITPAMAEAVFGARGRL
                     VLVPAAQRHFTLVGVEKLPMAALIAAAAAEAVAALGLGG"
     gene            complement(1511..>1994)
                     /locus_tag="PLQ29_11715"
     CDS             complement(1511..>1994)
                     /locus_tag="PLQ29_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPG87353.1"
                     /translation="VRRVAMLTGDSAASAERVAAEAGIAEVHHGLLPADKLERLEAIL
                     GQEQGRGSVVFVGDGVNDAPVLARADAGIAMGGGSDAAVESADVVLIGGDPSRVAEAI
                     DRARRTRRIVAQNIAFALVVKLAFLGLGAIGLAAMWEAVIADVGVALLAVLNSTRALK
                     "
BASE COUNT          284 a          747 c          635 g          328 t
ORIGIN      
        1 cggcccgcct ggccacgagc cacagctcgt cgaaggccgg gaggcccctg tagcgcgcgg
       61 tcccggccga ggctcccgcc agctccctgg cgaaggcctc cccgagccct gacgacgcgc
      121 cggtgactat cgctataccg ccactttcca tggggcgata ctagccccgg cggccaacgc
      181 tgtcaaaggg cgggcactcg cgccggatcg gggcttctcc ttctcacgcc gggctaatgt
      241 cgttatgctc atgacaactg cggaggtagc tcaatcatgc atagattgcc cgcgtgggta
      301 ggcctgggct cgggcgtcct ggcctttacc gccggtacga tgaacgtctt cacgctcgag
      361 agcgcgttgc accaggccgt tacccaccat tcgggtagtt cgtcgtcgat gatgctggcg
      421 ctgtcgctcg ggcactacgg attggccctg cgctttttgg ccatcatcct cgccttcgta
      481 gccggttcgg tcctgagcgg cttcattatc cgcgattacc atctacggct cggtagacgc
      541 tacggcgtca gcctgatgat cgagtcggcc tgcatcctgg tcgcgtggag cctggcctcc
      601 gaacggccgt tcgcgaccca gctactcctc gctggggcga gcgggctcca gaacgcgatg
      661 gctacgacct actcgggcgc cgtggtgcgc accacgcacc tgaccggcat ctataccgat
      721 atcggcgtca tagtgggcaa ccggctcgcc ggtatccgat cccagccagg caagctccgg
      781 cttctggctg gcatactcgc cagctttctg ctcggtggcc tggccaacag cctgctcttc
      841 ccgtatctcg ggaacggcgt actggccatc ccggccgcga tcaccggaac cctcgggttc
      901 ggctactggg tctataggca gctgtacctc aagcctcggg gccaggtcgc cgatacgccc
      961 gctggtgacg gggccgctcc cggcgccccg tagcggtttt cctgtcagcc gccgaggccc
     1021 agggccgcga cggcctcggc cgccgccgcc gctatcagcg cggccatcgg cagcttctcg
     1081 acgccgacga gggtaaagtg ccgctgggcc gcgggtacga gcaccagacg gccccgcgcg
     1141 ccgaagacgg cctcggccat cgccggcgtg atctcgccca tcatggcatt cggcgctatg
     1201 acgccgatcg ggccgagcac tacgtcgccg agcccgaccg agacgcgcac cgcgttctcg
     1261 ccggaggcgc cgcggtcggc gccggccttg agcatgcgct cggtggcgca cgagttcgcg
     1321 ccgagggcca ctatctcggc cgcgtcgcct atcagctccc tggtcttggc tatgagctgg
     1381 gccccgaggc cgccccccat gccgtccacc acgacgatcg tcttcctcat ggcccgaggg
     1441 tagccgcgag gcgagccagg gtcaagccgc ccgtcccgcg agccctcgag cgcgtagccg
     1501 caattccggt ctacttgagc gcccgagtcg agttgagcac cgcgagcagg gccacgccga
     1561 catccgctat gaccgcttcc cacatcgccg cgagccctat cgcgccgagg cccaggaaag
     1621 ccagcttgac gacgagcgcg aaggcgatgt tctgcgccac gatgcgcctc gtgcgcctgg
     1681 cccggtcgat agcttcggcc acccgcgacg ggtcgccgcc tatcagcacg acgtcggcgc
     1741 tctccaccgc ggcgtccgag ccgccgccca tcgcgatgcc ggcgtccgcg cgggccagca
     1801 ccggcgcgtc gttcacgcca tcgccgacga agacgaccga gccgcggccc tgttcctgcc
     1861 cgagtatcgc ctcgagccgc tcgagcttat ccgccggaag gagcccgtgg tgcacctcgg
     1921 ctatgccggc ttccgcggcc acccgctcgg ccgacgcggc cgagtcgccg gtcagcatcg
     1981 ccacgcgccg cacg
//
LOCUS       DAOPHV010000531         1986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_81298_length_1986_cov_3.378560, whole genome shotgun sequence.
ACCESSION   DAOPHV010000531 DAOPHV010000000
VERSION     DAOPHV010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81298_length_1986_cov_3.378560"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PLQ29_11720"
     CDS             complement(<1..1166)
                     /locus_tag="PLQ29_11720"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPG87354.1"
                     /translation="MDGQVRRTGEGIDGAADLLEVVTNAEPLESIPTAPSTADRAPSS
                     AERMSIRRYEALFKGLVQHASDVVLILESDGRVRFCGPSARSLLGYAPEEIEGRDFFS
                     LVGEGFAAEQRAMFSSNLVAEHPTFLAGFPLRKADGAELPVEAAASNYLRNPDIVGVI
                     MTLRDVSRQKKAEERALFYEYFDPLTRLPNRESFVRDVDRNVSIAMNRGRVFGVMALG
                     LDRFKMVNDMYGTKTGDEVLKNVAGALRSSFRNDDIVSRYRGDKFLALFPDIKSQDHI
                     KEIIAKARAAFIDPIPIGLGQKLNLSASMGVAFFPNDGKDAAELVRNAETALYMAKES
                     GRDGYRLFDARLNGEIIERQRIESDLGQAIGERRFEPFFQPKVDRSGFIVGVVLA"
     gene            1309..1391
                     /locus_tag="PLQ29_11725"
     tRNA            1309..1391
                     /locus_tag="PLQ29_11725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1342..1344,aa:Leu,seq:caa)
     gene            1414..1668
                     /locus_tag="PLQ29_11730"
     CDS             1414..1668
                     /locus_tag="PLQ29_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPG87355.1"
                     /translation="MECRPGCGACCVAPSVSSPIPGLPNGKPAGVPCPQLGDDYRCAL
                     FGLPERPAVCSSLRPEPEMCGASRAEALAYLESLERETAP"
     gene            1723..>1986
                     /locus_tag="PLQ29_11735"
     CDS             1723..>1986
                     /locus_tag="PLQ29_11735"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HPG87356.1"
                     /translation="MDAIRMWRNDTLGEAAVKALKKNGFDAVYVRTGAEAADLVAKHF
                     KKGASVGFGGSMTVKAIGVQGRAAAAGCELLDHNAPGLAPEAKG"
BASE COUNT          326 a          636 c          674 g          350 t
ORIGIN      
        1 gcgagcacca cgccgacgat gaagcccgag cggtcgactt tgggttggaa gaacggctcg
       61 aagcgccgct cgcctatggc ttggcccagg tcgctctcga tgcgctggcg ctcgatgatc
      121 tcgccgttca gcctcgcgtc gaacagacgg tagccgtcgc ggcccgattc cttggccatg
      181 tacagcgccg tctcggcgtt gcgtaccagc tctgcggcgt ccttgccgtc gttcgggaag
      241 aacgcgactc ccatcgacgc cgacaggttg agcttctggc cgaggccgat cgggatgggg
      301 tcgatgaagg cggcgcgcgc cttggctatg atctccttga tgtggtcctg cgacttgatg
      361 tcggggaaca gcgccaggaa tttgtcgccg cggtagcgcg agacgatgtc gtcgttgcgg
      421 aacgaggagc gtagcgcgcc ggccacgttc ttgagcacct cgtcgccggt cttcgtgccg
      481 tacatgtcgt tgaccatctt gaagcggtcg agtccgagcg ccatcacgcc gaagacccgg
      541 ccgcggttca tcgctatcga tacgttccgg tcgacgtcgc gcacgaagga ctcgcggttc
      601 ggcaacctgg tcagcgggtc gaagtactcg tagaacagcg cccgctcctc ggctttcttc
      661 tgcctggaca cgtcgcgcag cgtcatgatg acgccgacga tgtcggggtt gcgcaggtag
      721 ttcgatgcgg ccgcctccac cggcagctcg gcgccgtcgg ctttgcgtag cgggaagccg
      781 gcgaggaagg tcgggtgctc ggcgaccagg ttcgacgaga acatggctcg ctgctccgcg
      841 gcgaagccct cgccgaccag cgagaagaag tcccgcccct cgatctcctc gggcgcgtag
      901 ccgagcagcg agcgggcgct cggcccgcag aagcgcacgc ggccatcgga ctcgagtatg
      961 agcaccacgt cggaggcgtg ctgtacgagc cccttgaaca gcgcctcgta gcgccgtatg
     1021 gacatgcgtt cggcgctcga cggcgcgcga tccgccgtcg acggcgcggt cgggatgctt
     1081 tcgagcggtt cggcgttcgt cacgacttcg agtagatccg cggcgccgtc gataccctcg
     1141 ccggtgcgcc ttacctggcc gtccatcgct acagtgtacc cgggagcgcc ttgacgggca
     1201 agcgaaaagg gtttcggggg cttcggggta tacggatatc acgacgttcg gtggtcgcct
     1261 tgacgagccg tccgggggcg tagtacaaga agaggctctc cattcactgc cggcatggtg
     1321 aaatggcata cacggtagac tcaaaatctg ctgcccgcga gggcgtgagg gttcgagccc
     1381 ctctgccggt atcttctccg aacgggagcg accatggaat gcaggccagg ttgcggcgcg
     1441 tgttgcgtcg ccccgtccgt atcgtcgccc ataccgggcc tcccgaacgg taagccggcc
     1501 ggcgtcccct gcccccagct tggcgacgac taccgatgcg cgctgttcgg cctaccggag
     1561 cgacccgcgg tctgctcgtc gctacggccc gagcccgaga tgtgcggcgc ctcgcgcgcc
     1621 gaagcgctcg cgtacctcga atcgctcgag cgagaaacgg ctccttaggg tataatcgga
     1681 ttgtagtgct catccctact atatcgaacc caggaggatg ctatggacgc catcaggatg
     1741 tggaggaacg atacgctcgg cgaggcggcc gtcaaggcgc tcaagaagaa cggcttcgac
     1801 gcggtatacg ttagaaccgg tgccgaggcg gccgacctcg tcgccaagca tttcaagaag
     1861 ggcgcgagcg tcggcttcgg cggctcgatg accgtcaagg ccatcggcgt gcagggccgg
     1921 gccgcggcgg ccggctgcga actcctggat cataacgccc ccgggctggc gcccgaggcc
     1981 aagggc
//
LOCUS       DAOPHV010000532         1975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_81901_length_1975_cov_4.091667, whole genome shotgun sequence.
ACCESSION   DAOPHV010000532 DAOPHV010000000
VERSION     DAOPHV010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81901_length_1975_cov_4.091667"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PLQ29_11740"
     CDS             complement(<1..547)
                     /locus_tag="PLQ29_11740"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPG87357.1"
                     /translation="MRRYAIAACAILSAAVAAAFSWTSTPTIQVEAASLNGLASLSVR
                     CEVRGAEVWLDYQYRGTTPLDLTGLSPGSHVLALRADGYYEAVIALSLAADTKTTVTA
                     ALQRRTGYLDVRVSPPSATVVVDGESYAPGVIEVAAGQKTVTIKAFGYTEQTYSIYVP
                     ERLMASMSATLETASFEASGFR"
     gene            674..1672
                     /locus_tag="PLQ29_11745"
     CDS             674..1672
                     /locus_tag="PLQ29_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HPG87358.1"
                     /translation="MGVLIPIGSGKGGVGKTVFTANLGAVLASYGKTVILVDLDLGGA
                     NLHTCLGVRNNHPGVGSIIWKKEKRLENLVVPTGVDRLFLVPGDNLLPGTANLEFFTK
                     RRIMKELLELTADYVLVDLGAGASYNVIDFWLMSPDGIVVTAPEIPAILNAYSFLKTA
                     AFRLLFRSFKKGSEERERIVDFVVNRSEGQGDTFLAFAERLAASDGAAGSAAGLKALE
                     ELRRLRPRVVVNQGSGESDAAVAQRLREISSRNLGLGMEYIAYIMRDQNVPASIAGRS
                     PLAVSDPDAMFIRGVKAAADRILRAPEAPAPGLELDDEDLFSMIERSFDEQGSPAL"
     gene            1722..>1975
                     /locus_tag="PLQ29_11750"
     CDS             1722..>1975
                     /locus_tag="PLQ29_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87359.1"
                     /translation="MPRFVSSSRYPIARSISLAAFVAAAFVSSTAATPVEVVGLLSRA
                     ETIADSLDDEGLVGQLMMIGVEGVGRPSNASRLILERIRPG"
BASE COUNT          329 a          676 c          635 g          335 t
ORIGIN      
        1 gcctgaagcc gctcgcctcg aaactcgccg tctcgagggt cgcgctcatc gacgccataa
       61 gccgttccgg tacgtagatc gagtacgtct gctcggtata cccgaaagcc ttgatcgtca
      121 ccgttttctg cccggcggcc acctcgatga cgccgggcgc gtaggattca ccgtccacca
      181 cgacagtggc ggatggaggc gaaacgcgca cgtcgagata gcccgtcctg cgctgcagcg
      241 cggcagtcac cgtggtcttg gtatcggcgg cgaggctgag cgcgatgacg gcctcgtagt
      301 agccgtcggc gcgtagcgcc agcacgtgcg agcccggcga gaggccggtc aggtcgagcg
      361 gcgtggtgcc gcgatattgg tagtcgagcc agacctccgc gccgcgtacc tcgcaacgca
      421 ccgatagcga ggcgagcccg ttcaacgaag cggcttccac ctggatggtc ggcgtcgagg
      481 tccaggagaa ggccgcagcc acggcggccg aaaggatcgc gcaggcggcg atggcgtatc
      541 gtctcatgtc gtatactgta ccgcgccgcg tcggcgacca caagcgtccg aaccgcccgg
      601 acgcgatccg cgcttgtggc cccggccatt ccggcttaca atcgaacgat actcgaacgc
      661 gcaggagacc gacgtgggcg tactgattcc gataggctcg ggcaagggcg gggtaggcaa
      721 gacggtgttc acggccaacc tcggcgccgt gctcgcctcg tatggcaaga ccgtcatact
      781 cgtcgacctg gacctgggag gggcgaacct gcatacctgc ctcggggtac gtaacaacca
      841 cccgggcgtc ggctcgatca tctggaagaa ggagaagcgc ctcgagaacc tggtcgtgcc
      901 gaccggcgtc gaccgcctgt tcctcgtgcc tggcgacaac ctgctacccg gtacggccaa
      961 tctcgagttc ttcaccaagc gccgcatcat gaaggaactg ctcgagctga ccgccgacta
     1021 cgtgctcgtc gacctgggcg ccggcgcctc gtataacgtc atagacttct ggctcatgtc
     1081 ccctgacggc atcgtcgtca cggcgcctga gatccccgct atcctgaacg cctactcctt
     1141 cctgaagacc gccgcgttca ggttgctgtt ccggtcgttc aagaagggta gcgaggaacg
     1201 agagcgcatc gtcgatttcg tcgtgaaccg gtcggagggc cagggcgata ccttcctggc
     1261 cttcgccgag cggctagcgg cgtccgacgg cgccgcgggt agcgccgccg ggctcaaggc
     1321 gctcgaggag ctacgacgcc tgaggccgcg cgtcgtcgtc aaccagggca gcggcgagag
     1381 cgacgcggcc gtcgcccagc gcctccgcga gatatcgtcg cgcaacctcg gcctgggcat
     1441 ggagtatatc gcctatatca tgcgcgacca gaacgtgccg gcctccatcg ccggccgatc
     1501 gcccctggcc gtctctgacc ccgacgccat gttcatccgc ggcgtcaagg ccgccgccga
     1561 taggatactg cgagcgcccg aggcccccgc cccggggctc gagctcgacg atgaggacct
     1621 attctcgatg atagagcgct cgttcgacga acagggtagc ccggcgctct gatcccgggg
     1681 cggcccttgc cgcgagcgtc ctcggcgact atcatcgccc catgcctcgg ttcgtatcgt
     1741 cctcccgtta tcctatcgcc cgttccataa gcctggccgc gttcgtcgcg gccgccttcg
     1801 tgtcctcgac ggccgcaacg cccgtcgagg tcgtcgggct cctctcccga gccgagacta
     1861 tcgccgatag cctcgatgac gaggggctgg tcggccagct catgatgata ggcgtcgagg
     1921 gcgtcggtcg gccatcgaac gccagccgcc tcatcctcga gcgtatcagg cctgg
//
LOCUS       DAOPHV010000533         1966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_82457_length_1966_cov_4.152276, whole genome shotgun sequence.
ACCESSION   DAOPHV010000533 DAOPHV010000000
VERSION     DAOPHV010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82457_length_1966_cov_4.152276"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLQ29_11755"
     CDS             complement(<1..273)
                     /locus_tag="PLQ29_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87360.1"
                     /translation="MKKLFIVMAISIAFAANAFGQSAFENDMLIAPGTPNVTLGFDFG
                     LGLNAGFEYPLGSFDIDTARFTYGAKAVGSLRFAGGRHVLSDSNVLT"
     gene            complement(461..1198)
                     /locus_tag="PLQ29_11760"
     CDS             complement(461..1198)
                     /locus_tag="PLQ29_11760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPG87361.1"
                     /translation="MTGTTITIALCDDDDDLRAELLALVATMKPPEGFELDVAQYDSG
                     DGLANRLKAGDRFDIAILDMMMPGMDGIETARSIRAVDADTVIICLTNSPEYAIQSYR
                     VDAFDYLLKSGDASDLKATLSRAFSVVASREAARLHVKSGTTTHYVRCRDIEYVEVNG
                     KKLSYHLASGQVIDSYKPMRELEAEIAEHPRFFAIHRSIVVNLAYVAEIDPRFVRTVS
                     SDRLPVARGKYPSLEEAFLRHATTERA"
     gene            complement(1432..>1966)
                     /locus_tag="PLQ29_11765"
     CDS             complement(1432..>1966)
                     /locus_tag="PLQ29_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPG87362.1"
                     /translation="DSMVIVDLTGAVVEGKLRPSSDTKTHLVLYRSFAGIRGVCHSHS
                     PYAVAWAQAGRGIPCLGTTHADQTAGEIPCTPMIGPEAVARDYEEGTGELIVETFSGK
                     KPLESPMALVAGHGPFTWGKSAMDAVRNAAALEEIARMALLTYRLDPNASPLPDHVMR
                     KHFERKHGPGAYYGQPS"
BASE COUNT          340 a          575 c          664 g          387 t
ORIGIN      
        1 ggtcagcacg ttggagtcgg agagcacgtg cctgccgccg gcgaagcgga ggctaccgac
       61 ggccttggca ccgtaggtga agcgggcggt atcgatgtcg aagctaccga ggggatactc
      121 gaaaccggcg ttcaggccga gaccgaagtc gaagccgagc gtcacgttcg gcgtacccgg
      181 cgcgatcagc atgtcgttct caaaggcgga ctgaccgaat gcgttcgcgg cgaacgcgat
      241 cgagatggcc atgacgatga atagcttttt catgaaacct ccgaatggtt agcggcgccg
      301 cgtcggcgga gccgtcgatg atatcggccg cgtcctgtcc ggggcatgcg ctccgaaacg
      361 ggatagggcc gaaggtcaat gctattggaa ggagcgatga cgggcaacgc cttcgggcgc
      421 caattcgcgc ccgaaaaggc gctattcagg gaatgcccgg tcaggcccgc tctgtcgtcg
      481 cgtgacgcag gaacgcttcc tcgagcgatg ggtatttccc gcgggcgacc ggtagcctat
      541 cggacgagac ggttcgcacg aagcgagggt cgatctccgc tacgtaggcc aggttgacga
      601 cgatggagcg atggatggcg aagaaccgag gatgctcggc tatctcggcc tcgagctcgc
      661 gcatcggctt gtacgagtcg atgacctggc cggaggctaa gtggtacgat agcttcttac
      721 cgttcacctc tacgtattcg atgtcccggc atcggacgta gtgcgtcgtc gtgccggact
      781 tgacgtgcag cctcgccgcc tcgcgggagg cgacgaccga gaaggccctc gagagcgtcg
      841 ccttcaggtc gctcgcgtcc cccgatttga gcagatagtc gaaggcgtcg actctgtatg
      901 actgaatcgc gtattccggg gaattggtca ggcagatgat caccgtatcc gcgtctacgg
      961 cgcgtatcga cctggcggtc tcgatgccgt ccatgccggg catcatcata tcgagtatcg
     1021 ctatatcgaa gcgatcgccg gccttgagcc ggttcgcgag cccgtccccg gagtcgtact
     1081 gggctacgtc cagctcgaag ccctcgggcg gcttcatggt ggcgacgagg gcgagcagct
     1141 cggcgcggag gtcgtcgtca tcgtcgcaca gcgctatcgt gatggtggta ccggtcatgg
     1201 ctgttctgcc gctccatggt gatgtttgcc ggctcggccg tctctgatcc gtatcttact
     1261 ggattagcct acctatagca acgcgtcgtc gtcgagcacg gcttgggcgc gggtagcgta
     1321 gtgctcctct ttggggctta gtgtccgtga acaggatcga ctccagttca atggtatcga
     1381 aatctagcta gattctatag tatctcttag tggatacagg gcgggtatgg gtcagctcgg
     1441 ctgaccgtag tacgcgcccg gcccgtgttt ccgctcgaaa tgcttgcgca tgacgtggtc
     1501 gggcaggggg ctggcgttag gatcgagccg ataggtaagc agtgccatcc gggcgatttc
     1561 ctcgagggcc gcggcgttgc gcaccgcgtc catcgcgctc ttgccccagg tgaaggggcc
     1621 gtgcccggcc acgagcgcca tcggggactc gaggggcttc ttgccgctga aggtctcgac
     1681 gatgagctct ccggtgccct cctcgtaatc cctcgccacg gcctcggggc cgatcatcgg
     1741 ggtgcaggga atctcgccgg cagtctggtc ggcatgggtc gtgcccaggc agggaatgcc
     1801 gcgcccggcc tgggcccagg ctaccgcgta gggcgaatgg ctgtggcaga cgccgcggat
     1861 gcccgcgaag cttcggtaca ggacgagatg ggtcttggta tcggacgacg gccgcagctt
     1921 tccctcgacc accgcgccag tcaggtcgac gataaccata ctgtct
//
LOCUS       DAOPHV010000534         1963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_82644_length_1963_cov_3.015199, whole genome shotgun sequence.
ACCESSION   DAOPHV010000534 DAOPHV010000000
VERSION     DAOPHV010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82644_length_1963_cov_3.015199"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLQ29_11770"
     CDS             complement(<1..492)
                     /locus_tag="PLQ29_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87363.1"
                     /translation="MKALPTLLCILAATTATAALPAQPLPILEPPGGMRRGEPGIAAL
                     TLPAIDGPASAYTLSVRYADGADGPTYRGFAAPRGGPGKAGLGREIPFAGPSAASVRS
                     FVVLFMLSAPLDAPLGEASMVARGPDGEALAGARFSVGAREFGRQDLWLDEALTSIRV
                     DPDP"
     gene            580..1074
                     /locus_tag="PLQ29_11775"
     CDS             580..1074
                     /locus_tag="PLQ29_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87364.1"
                     /translation="MQLHLSMRRSGTALIYGIPLWYRLTASAIALTLAISSSISGAPS
                     LGALVVVAVAAVAALYQERWSFDAASGECSGRVGFVFAARGPSFKADEVARLRLDTFA
                     KGRLDQRDLPPDDKLPRGSQARLLVDLKDGESYMIDSVPVKRRPELELNARAIAEALG
                     ASLE"
     gene            1315..>1963
                     /locus_tag="PLQ29_11780"
     CDS             1315..>1963
                     /locus_tag="PLQ29_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPG87365.1"
                     /translation="MLILLADAFKADLPKRLEAYGEVTQDQGRVADAEIIIVRSKTKV
                     DKAMMDKAPKLKFIIRGGVGVDTIDVEYAKSKNVAVDNTPEASSLAVAELAFALMISL
                     PNHIAEADASMKQGKWLKKELERTELAGKTLGLVGIGRIAREVAVRAKAFGMRVIAYD
                     KFVEKSDAAEMMSLEQVLAQSDYISLHTPLTDETKGMINAKALASMKKGAYLVNTC"
BASE COUNT          350 a          690 c          642 g          281 t
ORIGIN      
        1 ggggtcggga tcgaccctga tgctcgtcaa cgcctcgtcg agccacaggt cctgtcggcc
       61 gaactcgcgg gcgccgacgg agaagcgggc gccggctagg gcctcgccgt ccggcccacg
      121 cgccaccatc gaggcttcgc cgagcggcgc gtcgagaggc gccgacagca tgaacaggac
      181 gacgaagctc cgtaccgacg ccgccgacgg ccccgcgaac gggatctccc ggccgaggcc
      241 ggccttgccc ggtccgcccc gcggcgccgc gaaaccgcga taggtcgggc cgtcagcgcc
      301 atcggcgtag cgcaccgata gcgtataagc gctcgctggg ccgtcgatag ccgggagcgt
      361 caaggcggcg atgccaggct cgccacgcct catgcccccg ggcggctcca ggataggcag
      421 gggctgggcg gggagcgccg cggtcgccgt agtggccgcg aggatgcaca acagagtcgg
      481 taaggccttc atggcgcgag tctatacggc cgcttcggcg agcgcaaccg ccagcgccgg
      541 cccttgcccg ggaaccggag ctacggctat agtcgactca tgcaactcca tctctccatg
      601 cgccgatccg gaaccgcgct gatctacggc atcccccttt ggtaccgtct aaccgcatcc
      661 gccatcgcgc tgacgctagc catctcatcg tccatctccg gcgccccgag cctcggcgcg
      721 ctcgtcgtcg tggccgtcgc cgcggtggca gcgctctatc aggagcgctg gagcttcgac
      781 gccgcgagcg gcgagtgctc cggcagggtg ggcttcgtct tcgccgcccg cggcccttcg
      841 ttcaaggccg acgaggtagc ccgcctcagg ctggatacct tcgccaaagg caggctcgac
      901 cagcgcgacc tgccgccgga cgacaagcta ccgcgcggct cccaggcgcg gctgctcgtc
      961 gacctgaagg acggcgagag ctatatgatc gactcggtgc cggtcaagcg caggccggag
     1021 ctcgagttga acgcccgcgc catcgccgag gcgctgggag cgagcctgga atagcggcgg
     1081 ccaggagccg ccggcggcgg ccaatagcgg cggccggcgt cggcctacca tcggtcgccg
     1141 gccgcccggc ttgacaaggc ttccttccat gataagatga ctgcatcgtc ccactcatag
     1201 cacgaatcca tgatgggttc gtctcgcccc gggccatcac cggcctcccg gggcggactg
     1261 tccatcgcca gagacgcgct acgatcgagc gacgatacaa caaaggagga tcccatgctt
     1321 atactgctcg ccgacgcctt caaggccgac ctgccgaagc gcctcgaggc gtacggagag
     1381 gtgacccagg accagggccg cgtcgccgac gccgagatca tcatcgtccg ctcgaagacc
     1441 aaggtcgaca aggcgatgat ggacaaggct ccgaagctca agttcatcat ccgtggcggc
     1501 gtcggcgtcg acacgataga cgtcgagtac gccaagtcca agaacgtcgc ggtcgataat
     1561 acccccgagg cctcgagcct cgcggtcgcc gagttggcct tcgcgctgat gatatcgctg
     1621 ccgaaccata tcgccgaggc cgacgcgtcg atgaagcagg gcaagtggct caagaaggag
     1681 ctcgagcgta ccgagctggc cggtaagacc cttggcctcg tcggcatcgg ccgcatcgcg
     1741 cgcgaagtcg ccgtccgcgc caaggccttc ggcatgcgcg tcatcgccta cgataagttc
     1801 gtcgagaagt cggacgccgc ggagatgatg agcctggagc aggtgctggc ccagtccgac
     1861 tacatcagcc tccacacccc gctgaccgac gagaccaagg gcatgatcaa cgccaaggcc
     1921 ctcgcctcga tgaagaaggg cgcctacctc gtcaacacct gcc
//
LOCUS       DAOPHV010000535         1961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_82773_length_1961_cov_3.284890, whole genome shotgun sequence.
ACCESSION   DAOPHV010000535 DAOPHV010000000
VERSION     DAOPHV010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82773_length_1961_cov_3.284890"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..949)
                     /gene="ugpA"
                     /locus_tag="PLQ29_11785"
     CDS             complement(59..949)
                     /gene="ugpA"
                     /locus_tag="PLQ29_11785"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HPG87366.1"
                     /translation="MVQQYEFKAKWLPYILVLPQMVIVFVFFFWPSLQGLWQSFFLQD
                     PFGGQVIFVGLENYIRLFTDPGYSYGESFGVSVAFAVCVAALSMILALFLATQANKKI
                     RFSSFYKTMMIWPYAVATMVAGVLWLFMFNPNVGIVAWFLKNRFGVEWQHLLNSGQAF
                     LLVTLAASWKQISYNFVFFLAGLQSVPVDFIEAAAIDGAGPMRRFWKIIFPLLSPTTF
                     YLLVMNLVYGFFETFPIIHQVTAGGPGKSTSILVYKVWRDGVINLDLGGSSAQSVILM
                     IMVIGLTVVQFRFIERRVHY"
     gene            complement(1013..>1961)
                     /locus_tag="PLQ29_11790"
     CDS             complement(1013..>1961)
                     /locus_tag="PLQ29_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPG87367.1"
                     /translation="PTITGYYSTSKGEMISIPFNSSTAVMYYNKDAFAKAGLDPEKPP
                     VTWPELFDAARKIKAAGYEAGFTTNWISWIQLENFSAWHNQPFGTKSNGFDGLNTELV
                     FNTPLVQRHLETIYQLSLDKTFIYGGRENKANPLFSSGQVGMHFESIGGYANFKNTCK
                     FDFGVARLPYYSDVAGAPQNSIIGGASLWVFNGKSKDEYKGVASFFSFLSSAEIQAFW
                     HQSTGYLPITMAAYDLTKSQGFYEKNPGPEVAIKQLLNKAPTPNSMGIRFGNFNIIRE
                     IEDQTWEDTLAGKISVKAGLDRMVREGNAKLRDFERLNK"
BASE COUNT          427 a          538 c          626 g          370 t
ORIGIN      
        1 gaggtagagg tgcggggtca gcctccgcag gcggttatgc tcggtcatcg atcgctcctc
       61 aataatgcac gcgccgctcg atgaagcgga attgtacgac ggtaagaccg atcaccatga
      121 tcatgaggat gaccgactgg gccgaggatc cgccgagatc caggttgatg acgccgtcgc
      181 gccatacctt gtacacgaga atgctcgtag acttgcccgg gcctccagcc gtcacctgat
      241 ggatgatcgg gaaggtctcg aagaagccgt agacgaggtt catcacaagc aggtagaacg
      301 tcgtcggcga caggagcggg aagatgatct tccagaaacg acgcatgggg ccagccccgt
      361 ctatcgccgc cgcctcgatg aagtcgaccg gcacgctctg tagccccgcc aggaagaaga
      421 cgaagttgta cgatatctgc ttccagctcg cggccagggt gaccagcagg aaagcctgcc
      481 cggagttgag cagatgctgc cactcgacgc cgaaacggtt cttgaggaac caggcgacta
      541 tgccgacgtt cgggttgaac atgaacagcc atagcacgcc ggcgaccatg gtcgccaccg
      601 cgtacggcca gatcatcatc gtcttataga acgacgagaa gcgtatcttc ttgttggcct
      661 gggtggcaag gaacagcgcc agtatcatcg acagggcggc tacgcacacg gcgaaggcga
      721 ccgatacccc gaaggattcc ccgtacgagt aacccggatc ggtgaagagc ctgatgtagt
      781 tctcgaggcc cacaaaaatg acctgcccgc cgaagggatc ctgcaggaaa aaggactgcc
      841 atagaccctg caacgacggc cagaagaaaa acacgaagac gatcaccatc tgcggcagca
      901 ccaggatata gggaagccac ttcgccttga actcgtattg ttgaaccatg gagcatcctg
      961 aggtaagaat cgagggggcg acacagcgtc gccccctcgt tacggagccg gattacttat
     1021 tgaggcgctc gaaatcgcga agcttggcgt tgccctcgcg aaccatcctg tccagtccgg
     1081 ccttgacgga gatcttgccg gcgagggtat cctcccaggt ctgatcctcg atctcgcgga
     1141 tgatattgaa gttgccgaag cggattccca tcgagttcgg ggtcggggcc ttgttgagca
     1201 gctgcttgat ggcgacctca gggcccgggt tcttctcgta gaatccctgg gacttggtca
     1261 gatcgtaggc ggccatcgtt atcggcaggt agccggtgct ctggtgccag aacgcctgga
     1321 tctcggcgga ggaaaggaac gagaagaagg acgcgacgcc cttgtactcg tccttcgact
     1381 tgccgttgaa gacccagagg ctcgcgccgc cgatgatgga gttctgaggg gccccggcca
     1441 cgtcggagta gtacggcaac ctggcgacgc cgaagtcgaa tttacaggta ttcttgaagt
     1501 tggcgtagcc gccgatggac tcgaagtgca tgcccacctg gccggacgag aacaacgggt
     1561 tggccttgtt ctcgcggccg ccgtaaatga aggtcttatc gaggctgagc tggtagatgg
     1621 tctccagatg gcgctggacc agcggcgtat tgaacacgag ctcggtattc aggccgtcga
     1681 acccgttaga cttggttccg aaaggctgat tatgccaggc cgagaaattc tcgagctgga
     1741 tccaggagat ccagttggtc gtgaaaccgg cctcgtagcc ggcggccttg atcttgcggg
     1801 cggcgtcgaa gagctcgggc caggtgaccg ggggcttctc ggggtcgagg ccggccttgg
     1861 cgaaggcatc cttgttgtag tacatgaccg cggtcgacga gttgaagggg atggagatca
     1921 tctcgccctt cgaggtcgaa tagtagccgg tgatggtggg g
//
LOCUS       DAOPHV010000536         1947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_83540_length_1947_cov_4.943446, whole genome shotgun sequence.
ACCESSION   DAOPHV010000536 DAOPHV010000000
VERSION     DAOPHV010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83540_length_1947_cov_4.943446"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1055..1762)
                     /locus_tag="PLQ29_11795"
     CDS             complement(1055..1762)
                     /locus_tag="PLQ29_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HPG87368.1"
                     /translation="MGIFDRMKTVISSNINDMINKAENPEKMLNQLVIDMNQQMIESK
                     KSVAMAIADEKKLERELIEQKRLSEDWERKAVIAVKAGRDDLAKEALLRKQEAENYYL
                     QLKPQWESQKASVEKLKETLRQLQNKIDEASRKKNMLVARAKRAEAQQKIQKTLASVS
                     GNTSAFDTFDRMAKKVDELEARADAQAELADLSQTSSLDKEFAKLEASGGGADVLLEE
                     LKRKMIGDGSAEPAGQA"
BASE COUNT          388 a          595 c          542 g          422 t
ORIGIN      
        1 cgacagacag agcatcgata cgccgaatcc gaggaaaaac ctgagaatcg cagccgcgat
       61 ttttttcatc gttaaaactg tagcatgagc catacggctt gacaagccga atggccacgc
      121 ggcgtccata ggcctatgga ttaccttgtt catacagaag agcacacaag ttatccacaa
      181 cggctcgaca aaaatagctg tattcggttt tgaccttttg gagcttccta gcgaggctca
      241 gcgtgcaagc catattcgct cgactgtaat tgctttccgg gcatgtattt atattttaaa
      301 caaagggccg tgtgtcgttc gaggctatgg tatacgattt aatacagaac gaaacggata
      361 cacacagttt tcggcacaaa caatgaacag gttttccaca agcgctattt tattcgatat
      421 ctagctttat acgaacattg aataggcgcc gggttcggcc ttgagccctc gagcggcctc
      481 gacggcgccg acgcttcgaa gtgcggctcg tagccgcgag acacgcatat ccaaggcgcg
      541 tcccgttatc gaacccatgc cagaaacggc tatgagcgtc gctctcataa cgggccggcc
      601 gcggttcgcc aacagcgcgg ccagcatgtt accgtcgccc gcgctcagga taaccgagcg
      661 gccggagcct ataaggagac ggccgtcgag gctagcgccg ccgctcaagg cccaacggcg
      721 cgaagacgtc gcggccctga ccctggctcg tagctcaggc tcgccccacg gctcgcatag
      781 ataatccgag cagccggcct cgaaacattc gtcggcgacc gacgccggcc cgcacgctag
      841 cgttacgaag aggtcggcgc gggatcgccg gtcaggcggc atagacagga accgaagggc
      901 cggaacgacc agtccatcaa aggcctcggc tacggatagg ccctcggcca agtcatcggg
      961 cgataaaaaa gagagggaga acggctcgcc gtcctccctc tccgctatag accgatagta
     1021 ctcttcgggg gtaccgacga agagtacccg cacgctaggc ctgaccggcc ggttccgcgc
     1081 tcccgtcgcc gatcatcttg cgcttcagct cttcgagcag cacgtcggcg ccgccgccgg
     1141 acgcctcgag cttggcgaat tccttgtcga gcgacgacgt ctgggacagg tcggccagct
     1201 ccgcctgggc gtcggcccta gcctccagct cgtccacctt cttggccatg cggtcgaagg
     1261 tatcgaaggc gctcgtattg cctgaaacgc tggcgagggt cttctgaatc ttctgttggg
     1321 cttccgcccg cttagccctg gccaccagca tgttcttctt gcgcgacgcc tcgtcgatct
     1381 tgttctggag ctgacgtagc gtctccttga gcttctcgac ggacgccttc tgggactccc
     1441 actgcggctt gagctgcaga tagtagttct cggcttcctg cttgcgcagg agcgcctcct
     1501 tggcgaggtc gtcgcggccg gccttgaccg cgatgacggc cttgcgctcc cagtcctcgg
     1561 acaggcgctt ctgctctatc aactcccgct caagcttctt ctcgtcggcg atagccatgg
     1621 ccacggactt tttcgactcg atcatctgct gattcatgtc gatgacgagc tggttgagca
     1681 tcttctcggg attctcggcc ttgttgatca tatcatttat gttggatgag atgaccgtct
     1741 tcatcctgtc gaatattccc atgatagcta gcctcctatg ctcggtcccg gaaagcgccg
     1801 aggacgctgt aatgcttgga cagggccaag ccgattgaat cgatggccgc ttcgaattcc
     1861 cgcttgtcca tcgtatcgta ctctaacgta tcgatcagga cgacgtcatc gccgtccagc
     1921 ccgtaggcgc cgtggaccat gtcggtg
//
LOCUS       DAOPHV010000537         1946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_83604_length_1946_cov_4.178213, whole genome shotgun sequence.
ACCESSION   DAOPHV010000537 DAOPHV010000000
VERSION     DAOPHV010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83604_length_1946_cov_4.178213"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="PLQ29_11800"
     CDS             complement(<1..1292)
                     /locus_tag="PLQ29_11800"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPG87369.1"
                     /translation="MLHSEFESLGLMPACLDALRHEGYEKPTPIQSASIPSLLAGRDL
                     LGCAQTGTGKTAAFALPTIQRLTTAANKRQPRKARSLVVAPTRELAIQIDASFAAYGR
                     KTELKRACVYGGVGKAPQIKAMERGVDVLVATPGRLLDLKNEGAIDLSAVEIVVFDEA
                     DRMLDMGFIHDVRKIIALLPPKRQTLLFSATMPTEIESLARSLLNDPVRVDIEPEKPA
                     VELIAQSLYYVEKGCKRDLLVHLIKELGVSRAVVFSRTKHGADRIAKALHVAGIPAGV
                     IHANKGQGNRTKTMDAFRAGETPVLVATDLAARGIDVDDISHVFNFDMPTEPETYVHR
                     IGRTARAGASGSAVSFCDTEEKRLLKQVERLLGTTIPVVKQHPYAGVKPVPTEEDLRD
                     AMFEERSKERMRARGQGRGGQGRGRQGSPLGSPRGAQA"
     gene            complement(1453..>1946)
                     /locus_tag="PLQ29_11805"
     CDS             complement(1453..>1946)
                     /locus_tag="PLQ29_11805"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPG87370.1"
                     /translation="VVSIGIGINAGPVVFGSVGARERMDFTSIGDTVNLAARLEGANK
                     TYGTKSLVAEAVYDRVKDDFLCREVDLLAVKGKNVPVRIYEVLQERDKASQKLIHIKE
                     GFEAGLAAYRSKRWSAAKKIFAKVSEAYGDEASEVFLRRVEVFEAAPPPADWDGVFRM
                     TVK"
BASE COUNT          307 a          625 c          655 g          359 t
ORIGIN      
        1 tgagcctgag cgcctcgggg ggagcccaaa ggggagccct gccgtccgcg accctggcct
       61 ccccgaccct ggccgcgcgc gcgcatgcgc tccttggacc gctcctcgaa catagcgtcg
      121 cgtaggtcct cctcggtcgg cacgggcttt acgccggcat aggggtgctg cttgaccacg
      181 gggatggtcg taccgagtag gcgctcgacc tgcttgagga gccgtttctc ctcggtatcg
      241 cagaacgata cggccgagcc ggaagcgccc gccctggcgg tgcggccgat gcggtgcaca
      301 taggtctccg gctcggtcgg catgtcgaag ttgaacacgt gcgatatatc gtcgacgtcg
      361 atgccacggg ccgcgaggtc ggtggcgacg agcaccgggg tctcgccggc gcggaacgcg
      421 tccatcgtct tggttcggtt gccctggccc ttgttggcgt ggatgacgcc cgccgggatg
      481 cccgcgacgt ggagggcctt cgcgatgcgg tcggccccgt gcttggtcct cgagaacacg
      541 accgccctgg agacgccgag ttccttgatc aggtggacga gcaggtcgcg cttgcagcct
      601 ttctcgacgt agtacaggct ctgcgcgatc agctcgacgg ccggtttctc cggctctatg
      661 tcgacacgga cggggtcgtt gagcaggctg cgcgccagcg actcgatctc ggttggcatg
      721 gtcgccgaga acagcagggt ctggcgcttc ggcggcagga gcgcgatgat cttgcgcaca
      781 tcgtggatga agcccatgtc gagcatgcgg tccgcctcgt cgaacacgac gatctcgacg
      841 gccgacaggt cgatcgcgcc ctcgttctta agatcgagga ggcggccggg cgtggcgacg
      901 agcacatcga cgccgcgctc catcgccttg atctggggcg ccttgccgac gccgccgtag
      961 acgcaggcgc gcttgagctc ggtcttgcgg ccgtaggcgg cgaagctggc atcgatctgg
     1021 atggccagct cgcgcgtggg ggccacgacg agggaacgag ccttgcgcgg ctgccgctta
     1081 ttcgccgcgg tcgtcaggcg ctggatggta ggcagcgcga acgcggcggt cttgccggta
     1141 ccggtctggg cgcagccgag caagtcgcgc ccggccagga ggctcgggat cgaagcgctc
     1201 tggatcggcg tcggtttctc gtagccttca tggcgcaacg cgtccaggca ggcgggcatg
     1261 aggccgaggg attcgaattc agaatgaagc atggatatcc ttagggcgag aaagaaggtt
     1321 tcggtcgatg gaacgcgtct atcgctaccg gtccgcgtac gttcctttta cggggccgcg
     1381 gatattctcg tccgtatgaa tatagcagaa actatcgcga acggatagcc cggtttcagg
     1441 ccgggcagcc gtctacttga ccgtcatcct gaacacgccg tcccagtcgg ccggcggagg
     1501 ggccgcctcg aagacctcga cgcggcgcag gaacacctcg ctcgcctcgt cgccatacgc
     1561 ttcgcttacc ttggcgaata tctttttcgc cgcggaccaa cgcttcgagc gatacgccgc
     1621 gaggccagcc tcgaagcctt ccttgatatg gatgagcttc tgggaggcct tatccctctc
     1681 ctggagcacc tcgtagatcc tgaccggcac attcttgccc ttgaccgcca gcaggtccac
     1741 ctcccggcac aggaagtcgt ccttgacccg atcgtagacg gcctccgcga cgagagactt
     1801 ggtgccgtag gtcttgttcg cgccctcgag cctggccgcg aggttgaccg tgtcgccgat
     1861 gctggtaaag tccatacgct cccgcgcgcc caccgagccg aagacgaccg gccccgcgtt
     1921 gatgcctatg ccgatggaca cgacgg
//
LOCUS       DAOPHV010000538         1943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_83784_length_1943_cov_3.875530, whole genome shotgun sequence.
ACCESSION   DAOPHV010000538 DAOPHV010000000
VERSION     DAOPHV010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83784_length_1943_cov_3.875530"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLQ29_11810"
     CDS             complement(<1..340)
                     /locus_tag="PLQ29_11810"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPG87371.1"
                     /translation="MNGSALSQYKGRRIAVGLSGGVDSSTALALLAEAGAHAFGLSMR
                     IWRPGAAPIAGVGDACYGPGELEDVAAAESLCAGLGVPYRSLDLSAEYEARILDYFKR
                     EYRAGRTPNPC"
     gene            504..1559
                     /locus_tag="PLQ29_11815"
     CDS             504..1559
                     /locus_tag="PLQ29_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87372.1"
                     /translation="MTIWRRAAAACAILAVAPLGAAAAPAAAVALDAFPDYLSVRSEF
                     LSAAITAAPAVALAFKPAYRETPAGRIRVSVERDGASFYVLFQRERDGGYPIGSAGNM
                     VIQRSAVTGYVTGVIWYLSDDGTSYVGLTPKNERTVVDYVVAGSLSRGDYALSRLVYQ
                     VFTGSFKAFHDATRGALDWSLVFGQPGPEASETFASALLAGGPSRAAKALLAAADDFT
                     AIGAYLGSVGRDGYEPEELREPRFAKAASFDDPRRPILRPIRAWSDDQGLPIEAAMAT
                     ILAAPPGGAYIALVGGVSDRPPRKLVVVPYRNGRGAYTLASFDADDSSAVDLAALVGS
                     MAGASVRLFSVPLPGTI"
     gene            1790..>1943
                     /locus_tag="PLQ29_11820"
     CDS             1790..>1943
                     /locus_tag="PLQ29_11820"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPG87373.1"
                     /translation="MDGLLIGFTSVSWQQGVMYLVGAALIILAVTKEYEPMLLLPIGF
                     GAILVNL"
BASE COUNT          295 a          683 c          622 g          343 t
ORIGIN      
        1 cgcaggggtt gggcgtacgg ccggcccggt actcgcgctt gaagtagtcg agtatcctgg
       61 cctcgtactc ggccgatagg tcgagcgagc ggtacggcac gccgagcccg gcgcagagcg
      121 actcggccgc ggccacgtcc tcaagctcgc cgggtccgta gcacgcgtcc ccgacgccgg
      181 ctatcggagc ggcgccgggc ctccatatcc tcatcgacag cccgaaggcg tgggcgccag
      241 cctccgcgag tagggccagg gcggtcgaag agtcgacgcc gcccgacagg cctaccgcta
      301 tgcgtcggcc cttatactga ctgagtgcgc ttccgttcat ctcctggata ctagcccgaa
      361 gcgggcgagg cggacaagct ggccgtatcg gcccgcggcg taaccatccg cgcgtattcg
      421 ccttttattg ccattgtggt atactcgcct acgtcgacca attccgctga agtcctcttt
      481 agcaatcaaa cggagcatga ctcatgacta tatggagacg cgcggcagcc gcctgcgcga
      541 tacttgccgt cgcccctctc ggcgctgccg ccgcgccagc ggccgccgta gccctggacg
      601 ccttccccga ctacctgtcg gtcaggtccg agttcctatc ggccgcgata accgccgccc
      661 cggccgtagc cctggccttc aagcccgcct atcgcgagac tccggccggc cgtatccgcg
      721 tctcggtcga gcgcgacggc gcgtccttct acgtactctt ccagcgcgag cgcgacgggg
      781 gctacccgat cggctcggcg ggcaatatgg tcatccagcg atccgcggtt acgggctacg
      841 tgaccggcgt gatctggtac ctgagcgacg acgggacgag ctacgtcggc ttgactccca
      901 agaacgagcg caccgtcgtc gactacgtcg tggccgggtc cctgtcgcgc ggcgactatg
      961 cgctgtctcg cctggtctac caggtgttca ccggcagctt caaggctttc cacgacgcga
     1021 cgaggggcgc cctcgactgg tcgctcgtgt tcggccagcc cggccccgag gcgtcggaga
     1081 ccttcgcctc cgccctcttg gccggcgggc caagtagggc ggccaaggca ctcctcgcgg
     1141 ccgccgacga ttttaccgcg atcggagcct acctaggctc ggtcggccgc gacggctacg
     1201 agcccgagga actccgcgag ccccggttcg ccaaggcggc ctcgttcgac gatccgcgca
     1261 ggccgatcct aaggcccatc cgcgcgtgga gcgacgacca gggcctgcct atcgaggccg
     1321 ccatggctac gatactggcg gccccgcccg gcggcgcgta catagcgctc gtcgggggcg
     1381 tctccgaccg gccgccgcgg aagctggttg tggtgcccta tcggaacggt cgtggcgcct
     1441 acacgctagc ctccttcgat gccgacgatt ccagcgccgt cgacctggcc gccctcgtag
     1501 gttccatggc cggcgcgtcc gtacgactat tcagcgtccc gttacccgga acgatatgat
     1561 tctgttgaca gataccgaat gagggcgtag cctcgagtca gagtcaggct gcggcccggg
     1621 ggggcgggac ggcgagcctg gcccggggta gcaccccgac tccatgaaac ctcctttcca
     1681 tggggccccc cgccgcccgc gcctagcgcg ggcggcttcg tcatccccct ccttgcctcc
     1741 gcggcgcgta ccgactacta tgtacccatc gaatcgggaa aggcggatta tggacggtct
     1801 actcataggc ttcacctcgg tgagctggca gcagggcgtg atgtatctgg tcggagcggc
     1861 gttgatcatt ctggccgtta cgaaggaata tgagccgatg ctgctcctgc ccataggctt
     1921 cggggccata ctcgtcaatc tgc
//
LOCUS       DAOPHV010000539         1942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_83848_length_1942_cov_4.254372, whole genome shotgun sequence.
ACCESSION   DAOPHV010000539 DAOPHV010000000
VERSION     DAOPHV010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83848_length_1942_cov_4.254372"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1572)
                     /locus_tag="PLQ29_11825"
     CDS             complement(<1..1572)
                     /locus_tag="PLQ29_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG87374.1"
                     /translation="MKLYRIHNVYCPLDHDERYLRERAAKELGVRPGHLAAFGIERRS
                     IDARDKRAVRFVYTLTAGLESPSRSLGAKAAELAPAEPYRFPAPSAEPSGKEGHRPII
                     VGAGPAGLFCALMLAEAGYRPIVIERGDAVDKRGLAVSSFWNGGELDPESNMQFGEGG
                     AGTYSDGKLTTSSGDGSGRAAQVLDELVAAGAPESIRFDGKPHIGTDTLVEVVKGLRR
                     KIESLGGEIRFRTRVEALAIEAGRARGVVLADGGRIDAGAVVLAIGHSSRDTFASLAA
                     QGVPMERKPFAMGLRIEHPQEMISRSQYGDAWDHPALGAADYKLTHRASDGRSVYTFC
                     MCPGGYVVDASSEPGLSACNGMSDYARDAPNANAAIVVSVRPDDFEGSGPLAGVEYQR
                     RWERLAWEAAREASGSPSAGHRGRSALPVQALGDFMAGRASACLGGVTPSVRGSYALA
                     ELSACLPPFVASGIREAVPAFDRRLSGFGRTDAALCGVETRTSSPVRVTRDDSLQSSL
                     RGLFPCGEGAGYAGGI"
     gene            1656..>1942
                     /locus_tag="PLQ29_11830"
     CDS             1656..>1942
                     /locus_tag="PLQ29_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPG87375.1"
                     /translation="MKKLRIGELSGLVGANASALRFWEERGITEPEKDSDNSYRLYSA
                     EDSCRFLMARRYRSFGFSVDDAKAMIGAADPEATDGLLARRLAELDEEARRA"
BASE COUNT          296 a          659 c          710 g          277 t
ORIGIN      
        1 gatgccgccg gcgtagccgg ctccctcgcc gcaggggaac agcccgcgta gcgacgactg
       61 cagggagtcg tccctggtta cgcggacggg cgacgaggtg cgcgtctcga cgccgcagag
      121 cgccgcgtcg gtcctgccga agccggacag cctgcgatcg aaggcgggca cggcctcccg
      181 tatgcccgac gcgacgaagg gcggtaggca ggcgcttagc tccgccagcg cgtaggagcc
      241 gcggacggat ggcgtcacgc cgcccaggca ggccgacgcc cggcccgcca tgaagtcgcc
      301 gagggcctgg accggcaggg cggacctccc gcgatggccg gccgaagggg agcccgacgc
      361 ctcgcgcgcg gcctcccagg ccagccgttc ccagcgccgc tggtactcga cgccggccag
      421 ggggcccgag ccttcgaagt cgtcggggcg caccgatacg acgatcgcgg cgttcgcgtt
      481 cggcgcatcg cgggcgtagt cgctcatgcc gttgcaggcg gataggccag gctccgaact
      541 ggcgtcgacc acgtagccgc cggggcacat gcagaacgtg tagacgctcc gcccgtcgga
      601 ggcgcggtgg gtcagcttgt agtcggcggc gccgagggcc ggatggtccc aggcatcgcc
      661 gtactgcgag cgcgatatca tttcctgagg gtgctcgatg cgcaggccca tggcgaaggg
      721 cttgcgctcc atcggcacgc cctgggcggc gagcgacgcg aaggtatcgc gcgacgagtg
      781 gccgatggcc agcacgacgg cgcccgcgtc gatacggccc ccgtcggcca agacgacgcc
      841 gcgcgcgcgg ccggcctcga tcgccagggc ctcgacccgg gtcctgaagc ggatctcgcc
      901 accgagcgac tctatcttgc ggcgcaggcc cttgaccacc tcgaccagcg tgtcggtgcc
      961 gatatggggc ttgccgtcga accgtatcga ctcgggcgcg cccgcggcca cgagctcatc
     1021 gaggacctgg gcggcgcggc cggacccgtc gccgctcgag gtcgtcagtt tgccgtccga
     1081 gtaggtgccg gcgccgccct cgccgaactg catgttcgac tccgggtcga gctcgccccc
     1141 gttccagaac gacgatacgg ccaggccgcg tttatcgacc gcgtcgccgc gctcgatgac
     1201 gatgggccga tagccggcct cggcgagcat gagcgcgcag aagaggccgg ccggacccgc
     1261 tccgactatg atcggtcggt gcccttcctt tcccgacggc tcggcggacg gcgccgggaa
     1321 gcggtatggt tcggcaggcg ccaactcggc ggccttggcc ccgaggctgc gggacggcga
     1381 ctcgagcccc gcggtgagcg tatagacgaa gcggacggcg cgcttgtcgc gcgcgtcgat
     1441 cgagcggcgc tcgatcccga aggccgcgag gtggcccggg cggacgccga gctccttggc
     1501 cgcgcgctcg cggaggtagc gctcgtcgtg gtcgagcggg cagtacacgt tatggatgcg
     1561 atagagcttc atggctccag tatacacgaa cgcgattcgc gatgctcgct cggaagactt
     1621 caaatccgag cacttttgtc gtagaatcgc gatcaatgaa gaagctgaga ataggcgagt
     1681 tgtccggtct cgtcggcgcg aacgcgtcgg cgctgcgctt ctgggaggaa cggggcatca
     1741 ccgagccgga gaaggactcg gacaatagct accgcctcta ttccgcggag gattcgtgcc
     1801 gtttcctgat ggcccggcgc tatcgcagct tcggcttctc ggtggacgac gccaaggcga
     1861 tgataggcgc cgcggacccc gaggccaccg acgggctcct ggccaggcgt ctggccgagc
     1921 tggacgagga ggctaggcgg gc
//
LOCUS       DAOPHV010000540         1940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_83997_length_1940_cov_2.635013, whole genome shotgun sequence.
ACCESSION   DAOPHV010000540 DAOPHV010000000
VERSION     DAOPHV010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83997_length_1940_cov_2.635013"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLQ29_11835"
     CDS             <1..310
                     /locus_tag="PLQ29_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87376.1"
                     /translation="EIVAGLNDLGGGTAEIDRAVVAVVDSSSSMAASVDSVDSKVAGN
                     NAAIESVRRLTMEARRDLELIAAGFDDILRRSATVRALGDRSGSCMQSLDEAISGLQG
                     "
     gene            585..1298
                     /locus_tag="PLQ29_11840"
     CDS             585..1298
                     /locus_tag="PLQ29_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPG87377.1"
                     /translation="MVGLAGGSGVTPFLSLAKAIADGDEDCSLTLLYGSRARGDMLFQ
                     EEFREIARRCGRFRLVNVLSDDELEGCEKGFISAELIRKHAPVDGDYSVFVCGPQAMN
                     DFVDAEIAELGLRPKFVRHELLGEYRNPGRNADYPEGARGRRFKLDVSVCGASYSIDC
                     YADETLLRAMERNGIAAPSECRSGECGWCHSRLVSGTVYVPEAVDGRRQADLQFGYVH
                     PCCSFPTSDLAIDVPPCRN"
     gene            complement(1295..>1940)
                     /locus_tag="PLQ29_11845"
     CDS             complement(1295..>1940)
                     /locus_tag="PLQ29_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87378.1"
                     /translation="LFVLGQDLLQSSRRALHDILGHSLTLVIFLLRRGLSEAARGDAI
                     DERAVLDECAERVREGLAGLDRRLGGQVSQEAVLSLALQGLLAVAPPDAIETELVSAG
                     GERALPPALVAAIRDACREAMTNALRHGKARRLSISLLWGATRLIVNVCDDGRGCDAL
                     EEGNGMTLMRDALAASGCDMSWQSEEGAGFQLSIRAPLPRDRGPAYAEAAAPKA"
BASE COUNT          304 a          648 c          691 g          297 t
ORIGIN      
        1 ggagatagtc gcgggcctga acgacctggg cgggggcacc gcggagatcg accgggccgt
       61 ggtggccgtc gtcgacagct cctcgagcat ggccgcctcg gtagactcgg tcgactcgaa
      121 ggtcgccggc aacaacgcgg ccatcgagtc ggtcaggcgg ctgaccatgg aggcccgtag
      181 ggacctcgag ctgatagccg ccggcttcga cgatatcctg aggcggtctg ctacggtccg
      241 tgcgctcgga gaccgcagcg ggtcgtgcat gcagtccctc gacgaggcca tctcgggcct
      301 ccagggctag cgccgctact cggccgtgat ctgcctgagg taggccacgg cccctgctcc
      361 gtcgccgcgc tcgatgaggt cgtagtagcg cctgagcctg agcgccgtct cgctcgggtc
      421 cctgacgtgg gaggaggctt cccagaaggc caggcccgcg ttcttgaagg cgttgaagat
      481 gagcgggatg accgtgttgc ccgagtcgac gcagacgcgg tggtggaacc tgaagtgcag
      541 cagcgccagg gcctggccgt cggccgctgc gcgacgccga gcgtatcgtc ggcctcgccg
      601 gcggcagcgg cgtcacgccc ttcctgtcgc tcgccaaggc gatagccgac ggcgacgagg
      661 actgctccct gaccctgctc tacggcagcc gcgccagggg cgacatgctg ttccaggaag
      721 aattccgcga gatcgcccga cgttgcggcc ggttccggtt ggtcaacgtg ctgagcgacg
      781 acgagctcga aggctgcgag aagggcttca tcagcgccga gctcatcagg aagcacgcgc
      841 ccgtcgacgg cgactattcc gtattcgtct gcggcccgca ggcgatgaac gacttcgtcg
      901 acgccgagat cgccgaactc ggcctcaggc cgaagttcgt gcgccacgag ctgttaggag
      961 agtacaggaa ccccggccgg aacgccgact atccggaagg cgccaggggc aggcgcttca
     1021 agctggacgt ctcggtctgc ggcgcgtcct attcgatcga ctgctacgcc gacgagaccc
     1081 tgctgcgcgc gatggagcgg aacggtatcg cagccccgtc ggaatgcagg agcggggagt
     1141 gcggctggtg ccactcgcgg ctggtgagcg gcacggtgta cgtgcccgag gccgtggacg
     1201 gaagacgcca ggccgacctc cagttcggct acgtgcatcc ctgctgctcg ttcccgacca
     1261 gcgacttggc tatagacgtt ccgccatgta ggaactaagc cttaggagcc gcggcctccg
     1321 cgtaggcggg gccgcgatcc cgaggcaacg gcgcccggat cgatagctgg aacccggcgc
     1381 cttcctcgct ctgccaggac atgtcgcaac cggacgcggc gagcgcgtcc ctcatgaggg
     1441 tcatgccgtt tccttcctcg agggcgtcgc agccgcggcc gtcgtcgcag acgttgacga
     1501 taagccgcgt cgcgccccag aggaggctga tgctcaggcg cctggccttg ccgtggcgca
     1561 gcgcgttggt catcgcctcg cggcacgcgt ctcggatggc cgctacgagc gccggcggaa
     1621 gcgcccgctc gccgccggcg ctgacgagct cggtctcgat ggcgtcagga ggggcgaccg
     1681 ccaggagccc ttgcagggcc agcgatagta cggcttcctg cgatacctgg ccgccgagcc
     1741 tgcggtcgag gccggcgagc ccctcccgta cgcgctcggc gcattcgtcg agaacggcgc
     1801 gctcgtcgat cgcgtcgccc cgggccgctt cgctcaggcc gcgcctgagc agaaagatga
     1861 ccagcgtcag cgaatggccg aggatgtcgt ggagggcgcg gcgcgacgac tgcagcaggt
     1921 cctgccccag gacgaagagc
//
LOCUS       DAOPHV010000541         1937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_84141_length_1937_cov_4.021785, whole genome shotgun sequence.
ACCESSION   DAOPHV010000541 DAOPHV010000000
VERSION     DAOPHV010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84141_length_1937_cov_4.021785"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1134
                     /locus_tag="PLQ29_11850"
     CDS             217..1134
                     /locus_tag="PLQ29_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87379.1"
                     /translation="MRLPPWFERFMAPEADRAAVLAAAIAELGVRPRILSIAGGRFVV
                     ARPRGPRRDGRYRVKVITAHHDRVPGTPGALDNSAACLQLVRFLAEARDAFNTVVVFT
                     DKEELGPAPHEYPAHRDDPARRSASPTEQGSYELGKVLAKELGPGAPMVFPLDVTGRG
                     DALVLSRAAEGLALRAGWEPWANAAVDSLASETAAMAATAARLMAGRGRALMASVPFG
                     EDLGFMLAGLPALTVTVLPRAEAEALAADGALPAWASPSAPGSRVPATWRVLHGPDDR
                     VGLYDDAAFELVARFLERLAALRVPAMIP"
     gene            1216..>1937
                     /locus_tag="PLQ29_11855"
     CDS             1216..>1937
                     /locus_tag="PLQ29_11855"
                     /inference="COORDINATES: protein motif:HMM:NF020028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HPG87380.1"
                     /translation="MANANEIPARRDVPAEHRWNLSRLSKDEASWEADLKSLEGMVGR
                     VAAFRGTLASSSDSLYAALSFYKEYSMLDERLGYYAHLRQTEDEGDSSSRGRMARYMM
                     AATKASGEWSWLTPELQRVPDASMKAWMAEERYAEFRVYLSKILRYKPHVLSAEEERL
                     LALASDAATTPQEAFSVLTNVDLDFGVVDTPEGPRPLSQSTFSSFMHSPDRKVRREAY
                     ARFYAEYDAHKNTIAQLYAGQC"
BASE COUNT          301 a          701 c          640 g          295 t
ORIGIN      
        1 ggcgatcgcc tcggagacct tgatgcccga cgggaagtcg agcgtgcggc ccgaagggta
       61 gcgtatggtc accagatgca tgttctctcc ttctggaacc gccgggcccc gtggcccgac
      121 gatttgcttt tatgcgaata taaccatagt atagaccgac tcgagcgggg gcgccatgaa
      181 gaaagctatc agtaccatcg ctgaacgggc gcgcgcgtga ggctaccgcc ctggttcgag
      241 cggttcatgg cccccgaggc cgaccgagcc gccgtcctcg ccgcggccat cgccgagcta
      301 ggcgtccgcc cgaggatcct gtcgatcgcc ggcggtcgct tcgtcgtcgc caggccgcgc
      361 ggcccgaggc gcgacgggcg ctaccgcgtc aaggtgatca ccgcccacca tgaccgagtg
      421 ccgggcaccc cgggcgcgct cgacaactcg gccgcctgcc tccaactcgt gcgcttcctg
      481 gccgaggccc gcgacgcctt caataccgtc gtcgtgttta ccgataagga ggaactcgga
      541 ccggcgcccc atgagtaccc ggcgcaccgc gacgaccccg cgcgccgctc ggccagtccg
      601 accgagcagg gctcgtacga gctcgggaaa gtcctggcca aggagctcgg tcccggggcg
      661 ccgatggtgt tccctctcga cgtgaccggg cgcggcgacg ccctcgtcct gtcgcgggcc
      721 gccgagggtc tggccctgcg cgccggctgg gagccctggg ctaacgccgc cgtcgactcg
      781 ctcgcctcgg aaaccgccgc gatggccgcg acggcggcca ggttgatggc cggtcgcggc
      841 cgggcgctca tggcgtcggt gcctttcggc gaggacctcg gcttcatgct ggcgggcctg
      901 ccggccctga ccgtgaccgt gctgccgcgg gccgaggccg aggccctcgc ggccgatggc
      961 gccctgccgg cctgggcctc cccttcggcg ccgggcagcc gcgtaccagc gacctggcgt
     1021 gtcctccacg ggccggacga ccgcgtcggg ctgtacgacg atgccgcctt cgagctggta
     1081 gcccgcttcc tcgagcggct ggcggcgctg cgcgtaccgg cgatgatacc gtgaggccgc
     1141 ttggatcggc ttttcgaatc cggtatagtg acgcgtcggt actacgaacg gaaccactaa
     1201 aaaggagtga tatacatggc gaacgcaaac gaaatcccgg cccgcaggga cgtacccgcg
     1261 gagcatcgct ggaacctgtc caggctgtcg aaggacgaag cctcctggga ggccgacctc
     1321 aagagcctcg agggcatggt cggccgcgtc gcggccttcc gcggcaccct cgcctcgtcg
     1381 tcggactcgc tgtacgcggc cctgtccttc tataaagagt actcgatgct cgacgagagg
     1441 ctcggctact acgcccacct caggcagacc gaggacgagg gcgactcgag ctcgcgcggc
     1501 cgcatggccc gctacatgat ggccgccacg aaggcctcgg gcgagtggag ctggctgacc
     1561 cccgagctgc agcgcgtccc cgacgcctcg atgaaggcct ggatggccga ggaacgctac
     1621 gccgagttcc gcgtctacct gtccaagatc ctgcgctaca agccccacgt actgtcggcc
     1681 gaggaggagc gcctgctcgc cctcgcctcg gacgcggcga ccaccccgca ggaagccttc
     1741 tccgtgctga ccaacgtcga cctcgacttc ggcgtcgtcg ataccccgga gggcccgagg
     1801 ccgctgtccc agtcgacctt ctcgtccttc atgcacagcc ccgaccggaa ggtgcgccgc
     1861 gaggcctacg ctcgcttcta cgccgagtac gacgcgcaca agaacaccat agcccagctc
     1921 tacgccggcc agtgcaa
//
LOCUS       DAOPHV010000542         1936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_84209_length_1936_cov_3.314726, whole genome shotgun sequence.
ACCESSION   DAOPHV010000542 DAOPHV010000000
VERSION     DAOPHV010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84209_length_1936_cov_3.314726"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PLQ29_11860"
     CDS             complement(<1..755)
                     /locus_tag="PLQ29_11860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPG87381.1"
                     /translation="MNMLFIEAGEAGLALGRGDPRRKHISSVLKKGPGDELAAGRADG
                     RLGVARIEADDEDGMTLSFVPGPEAPPLRPVTLLLGFPRPIQANRILKDLASLGVARI
                     MLSGTELGEKSYFQSDFFKNREFRSALIEGAEQAGNPRLPVVETAWTLARALDAIVPE
                     ADDAARDGASRFWAFDPYRAERPFGSVALSDSGPERPLVLAIGSERGWTDAELDLLSA
                     SGFSFASLGDRILKTATACVVAVAMALGKLGYI"
     gene            complement(1116..>1936)
                     /locus_tag="PLQ29_11865"
     CDS             complement(1116..>1936)
                     /locus_tag="PLQ29_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional NADH-specific enoyl-ACP
                     reductase/trans-2-enoyl-CoA reductase"
                     /protein_id="HPG87382.1"
                     /translation="AAVVALARERGLVFDQVVYSLASPVRVDPATGVMHRSVLKPLGE
                     PFTGRTFDVVDGRMSEVTIQPATDEEVEATVKVMGGEDWQLWIGALSEAGVLAPGCVT
                     LAYSYVGPAHTAGIYRGGSIGRAKEHLERTAAAISDRLAASGGQAFVAVNKAVVTRAS
                     AVIPVVSLYVAALFKTMKELGLHEDCQDQMLRLYRERLLAPSAVPTDEEGRIRLDDLE
                     MRADVQEATARRFSAATEENLAETVDLDGFRHDFLEAHGFEVPGVDYDADVAFR"
BASE COUNT          292 a          615 c          718 g          311 t
ORIGIN      
        1 cagatgtagc cgagcttgcc gagggccatg gccacggcga cgacgcaggc tgtcgcggtc
       61 ttgaggatgc ggtcgccaag gctcgcgaag gagaagccgc tggccgatag caggtcgagc
      121 tcggcgtccg tccagccgcg ctcgctgcct atggccagga cgagcggccg ttccggcccg
      181 gagtcggaca gggcgaccga gccgaaggga cgctcggccc ggtaggggtc gaaggcccag
      241 aaccggctcg cgccatcgcg cgcagcgtcg tcggcctccg gaacgatagc gtcgagggcg
      301 cgggccaggg tccaggcggt ctcgacgacc ggcagcctcg ggttgccggc ctgctcagcc
      361 ccctcgatga gcgcggagcg gaactcgcgg ttcttgaaga agtcgctctg gaagtacgat
      421 ttctcgccga gctcggtgcc cgacagcatg atgcgcgcca cgccgaggga cgcgaggtcc
      481 ttgaggatgc ggttggcctg gatgggccgc gggaagccga ggagcagggt gacgggccgc
      541 aggggcggcg cctcggggcc gggtacgaag gacagcgtca tgccgtcctc gtcgtcggcc
      601 tctatccgcg cgacgccgag cctgccgtcc gctcgcccgg cagccagctc gtcgccaggg
      661 cccttcttga gcacggagct tatatgcttg cggcgcgggt cgccgcgccc gagagcgagg
      721 ccggcctcgc cggcctcgat gaagagcatg ttcatgggag cctaggatag ccgaaggact
      781 cgagaaaaaa aagaccgccg cgttgctcga tggacggcgc cggaatgggc gcctttcggg
      841 cccgccatcg cggaggcggg ccgacgcgcc gatggctgat cggcgcgtat ccgttaggaa
      901 tgggtggtcg atgcctttcc tgatacctcc tagggtccat tcgagtaacg cggcggccgg
      961 caggtccgca ttcgtccccg gttcgggcgg tcgtctcccc gggggattgt ccaatcaaat
     1021 tggccatgga gacgcagagc gaggttagct tctaactaaa actcgttttc tccgtgtctc
     1081 cgtgtctccg tggttagtct ttccttatgt tggccctatc tgaacgccac gtcggcatcg
     1141 tagtcgacgc cgggtacttc gaagccgtgc gcctcgagga agtcgtgcct gaaaccgtcg
     1201 aggtcgacgg tctcggcgag gttctcctcg gtggccgcgg agaagcggcg ggcggtcgcc
     1261 tcctgcacgt cggcgcgcat ctcgaggtcg tcgaggcgta tgcggccctc ctcgtcggtc
     1321 ggcacggcgc tgggcgcgag gaggcgctcg cggtacagcc tgagcatctg gtcctggcag
     1381 tcctcgtgga ggccgagctc cttcatggtc ttgaagaggg cggccacata gagggagacg
     1441 acggggatga cggcgctggc gcgcgtgacg acggccttgt tcacggccac gaaggcctgg
     1501 ccgcccgagg cggcgagccg gtcggagatg gccgcggcgg tccgctcgag atgttccttg
     1561 gcgcggccta tggaaccgcc gcggtagatg cccgcggtgt gcgccggtcc cacgtaggag
     1621 taggcgaggg tgacgcagcc gggggccagg acgccggcct cgctcagggc gccgatccat
     1681 agctgccagt cttcgccgcc catcaccttg acagtcgcct cgacctcctc gtcggtggcg
     1741 ggctggatgg tcacctcgct catgcggccg tcgacgacgt cgaaagtgcg gccggtgaac
     1801 ggttcgccga ggggcttgag cacggaacgg tgcatgacgc cggtcgccgg gtcgacgcgt
     1861 acggggctcg ccagggagta gacgacctga tcgaagacga ggccgcgctc gcgggccagg
     1921 gcgacgacgg ccgccc
//
LOCUS       DAOPHV010000543         1934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_84336_length_1934_cov_4.361362, whole genome shotgun sequence.
ACCESSION   DAOPHV010000543 DAOPHV010000000
VERSION     DAOPHV010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84336_length_1934_cov_4.361362"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PLQ29_11870"
     CDS             <1..536
                     /locus_tag="PLQ29_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87383.1"
                     /translation="SWDDSLEGASEDRARLGLLEGVEPPYRSFNGITSRFGAHFILRI
                     DAILMKERQYDAIVGGVRARDFPRVPVDYGYRDSKSFWYTRFRKPLGVSVPARPGMDL
                     ASVKYAADRIDPEVSFSDEACEALRKIPRIFLKTALTGIAEQAKSMDLPRVGGKLVVD
                     AEVLAIVNDKRNAEKRG"
     gene            complement(533..1534)
                     /locus_tag="PLQ29_11875"
     CDS             complement(533..1534)
                     /locus_tag="PLQ29_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HPG87384.1"
                     /translation="MALFESYERRVGQILPLLKNYGIGSIEEAKKVCDDKGVDVAGIV
                     SGIQTICFENAKWAYTVGAAIAIKKGERDAVSVAKSLGEGLQAFCIPGSVADDRKVGL
                     GHGNLAAMLLSDETTCFCFLAGHESFAAAEGAIGIARSANRARKTPLKVILNGLGKDA
                     AQIISRINGFTYVQTQFDYYTGALKIVKEWKYSDGEKAAVRCYGADDVREGVAIMWKE
                     EVDVSITGNSTNPTRFQHPVAGTFKKECVDRGRKYFSVASGGGTGRTLHPDNMAAGPA
                     SYGMTDTMGRMHSDAQFAGSSSVPAHVEMMGLIGMGNNPMVGASVAVAVAVSEAMRR"
     gene            complement(1550..>1934)
                     /locus_tag="PLQ29_11880"
     CDS             complement(1550..>1934)
                     /locus_tag="PLQ29_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87385.1"
                     /translation="LVCDAINTAMRELFLQIIYGRSQSAFSEGGLPIGAGLEDLGKGL
                     RSQVATMYATLKKGPRYLEMAEGYVTRIGVDKDGAIIGYEFVSLGKMMDAIKKGVDAN
                     EALAKAKGSYGRFAEAVKHLDPRHE"
BASE COUNT          306 a          618 c          645 g          365 t
ORIGIN      
        1 gcagctggga cgactcgctc gagggtgcct ccgaggatag ggctcgcctc ggcctgctcg
       61 agggcgtcga gcctccgtac cggagcttca acggcatcac gagcaggttc ggcgctcact
      121 tcatcctgcg tatcgacgcg atcctcatga aggagcggca gtacgacgcc atcgtgggcg
      181 gcgtccgagc gcgtgatttc ccgcgcgttc cggtggatta tggctacagg gattccaaga
      241 gcttctggta cacccgattc aggaagccgc tcggcgtatc ggtcccggcc aggccaggca
      301 tggacctcgc ctcggtcaag tacgccgccg acaggatcga tcccgaggtg tctttcagcg
      361 acgaggcctg cgaggcgctc cggaagatac cccgcatctt cctcaagacg gccctgacgg
      421 gtatagccga gcaggccaag tccatggacc ttcccagggt gggcggaaag cttgtcgtcg
      481 acgccgaggt actcgccatc gtcaacgaca agcgtaacgc ggagaaacgg ggctagcgcc
      541 tcatcgcctc cgataccgcg acagccacgg cgacggaggc tccgaccatg gggttgttgc
      601 ccataccgat gaggcccatc atttcgacgt gggccggtac cgaggaggag ccggcgaact
      661 gggcgtcgga gtgcatgcgg cccatggtat cggtcatgcc gtaggaggcg ggaccggcgg
      721 ccatgttgtc cggatggagg gtgcggcccg tgccgccgcc cgaggccacg gagaagtact
      781 tgcggccccg gtcgacgcat tccttcttga aggtgccggc caccgggtgc tggaagcggg
      841 tggggttggt cgagttgccg gtgatggaga cgtcgacctc ctccttccac atgatggcca
      901 cgccctcgcg cacatcgtcg gcgccgtagc agcgcaccgc cgccttttcg ccgtcggagt
      961 atttccactc cttgacgatc ttgagagcgc ccgtgtagta gtcgaactgg gtctgcacgt
     1021 aggtgaagcc attgatgcgg ctgatgatct gggcggcgtc cttgccgagg ccgttcagga
     1081 tcaccttgag cggcgtcttc cgggcgcggt tggcgctgcg ggcgatgccg atggctccct
     1141 cggcggctgc gaacgactcg tggccggcga ggaaacagaa gcacgtggtc tcgtcggaca
     1201 ggagcatcgc cgccaggttg ccgtggccga gacccacctt gcggtcgtcc gcgacggagc
     1261 ccgggatgca gaaggcttgg agcccctcgc cgaggctctt ggcgacggac acggcgtccc
     1321 gttcgccctt cttgatcgcg atggcggccc ctaccgtgta ggcccacttg gcgttctcga
     1381 agcagatggt ctggatgccc gacacgatgc cggccacgtc cacgcccttg tcgtcgcaga
     1441 ccttcttggc ctcctcgatg gagccgatgc cgtagttctt gagtaggggt aggatctggc
     1501 caaccctgcg ctcgtagctt tcaaacagtg ccatgattcg ttccctccct tactcgtgcc
     1561 tggggtcgag gtgcttgacc gcctcggcga agcggccata gctacccttg gccttggcga
     1621 gggcctcgtt cgcatcgacg cccttcttga tcgcgtccat catcttgccg aggctcacga
     1681 actcgtagcc gatgatcgcg ccgtccttgt ccacgccaat gcgggtcacg tagccctcgg
     1741 ccatttccag gtagcgcggg cccttcttga gggtggcgta catcgtggcc acctggctgc
     1801 ggaggccctt gcccagatcc tcgagcccgg cgccgatggg gaggccgccc tcggagaagg
     1861 ccgactggct ccgtccgtag atgatctgga ggaagagttc gcgcatcgcc gtgttgatcg
     1921 cgtcgcagac gagg
//
LOCUS       DAOPHV010000544         1920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_85267_length_1920_cov_3.073458, whole genome shotgun sequence.
ACCESSION   DAOPHV010000544 DAOPHV010000000
VERSION     DAOPHV010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85267_length_1920_cov_3.073458"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLQ29_11885"
     CDS             complement(<1..296)
                     /locus_tag="PLQ29_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87386.1"
                     /translation="MTVGQKASVSLLVSTVLAAGFAALAYSGLFSVIETRFFDERVRR
                     SVDASVDGLLESTDAYHESNAARFEALLGNDFVKRSFLTNQSAQDAFDRTRAFGL"
     gene            462..>1920
                     /locus_tag="PLQ29_11890"
     CDS             462..>1920
                     /locus_tag="PLQ29_11890"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG87387.1"
                     /translation="MHIRDFSLKTKVLVLTQAGLAIIAAVIAVMYVRDIAKVAQANIV
                     EKSRAITLTVEATRDSMAEKLAAGVIADLETLAAEGDRDKLISAVPIITAMDVAARNA
                     DEGGYSFRVPKVRPRNPANEPDEVELEALKLLSGGLEEYVVREPRRIRYFRPVRLSQD
                     CMLCHGDPAGSIDPTGGEREGWRVGEVHGAFEVISSLGAAKLVGKQATVNVASFAGGV
                     MLLLGFALFLIIRVVLKPIVTYVDAFKSASTGDLTVRATVRARDEIGRIAAYFNDFIA
                     TLEHMVGEVKSVTQTTENISLDLAASSEETAASLHEIRINTKGMKNKIVHLDGEVSSS
                     SKSAEDVDSYLSRLSELIADQASAINQSSASIEQMSASINNIAMAAEEKLRVANELES
                     TALEGQSEMEETEQTIKKVSDSASVIMEMIQIIQDIASKTNLLAMNAAIEAAHAGDFG
                     KGFAVVADEIRNLAESSAESAKQITQSLGEVTDYIA"
BASE COUNT          406 a          606 c          604 g          304 t
ORIGIN      
        1 aggccgaagg ccctggtacg atcgaaggcg tcctgggcgc tctggttggt caggaagcta
       61 cgcttgacga agtcattgcc gagcagggcc tcgaagcggg ctgcgttcga ctcgtggtat
      121 gcgtccgtcg actcgagcag cccgtcgacc gaggcatcga ccgaccgcct cacccgttcg
      181 tcgaagaacc gggtctcgat gaccgagaac agccccgagt acgcgagcgc tgcgaagccg
      241 gccgccagca cggtcgagac gagtaggctt actgaggctt tctgccctac ggtcatcgct
      301 gtccttactt gctatctatt cgtttcatta tcggcatacg gggacgtttt ataaagccgc
      361 tcagatcgtc gcttgccgct cggcgctcgt gatcatatac tcatcctaga cgcgagccaa
      421 gctcggcaag cgagcgcccg cagatcgaac caggagacca gatgcatatc cgagacttca
      481 gcctcaagac gaaggtgctc gtactgaccc aggccggact ggctatcata gccgcggtca
      541 tagcggtcat gtacgtgagg gacatagcca aggtggccca ggcgaatata gtcgaaaaga
      601 gccgcgccat cacgctgacg gtagaggcga cccgagactc gatggccgag aagctggcgg
      661 ccggcgtcat cgcggacctc gagaccctgg ccgccgaggg agaccgcgac aagctgatct
      721 ccgccgtgcc cataataacg gcgatggacg tggcggcccg caacgccgat gaaggcggct
      781 acagcttcag ggtacccaag gtacggccga ggaacccggc caacgagccc gacgaggtcg
      841 agctcgaggc gctcaagctt ctgagcggcg gcctcgagga atacgtcgtg cgggagcctc
      901 gacgcatacg gtacttcagg ccggtcaggc tgtcgcagga ctgcatgcta tgccacggcg
      961 acccagccgg ctcgatcgac ccgaccggcg gcgagcgcga gggctggcgc gtcggtgagg
     1021 ttcacggcgc cttcgaggta atcagctccc tcggcgcggc caagctcgtc ggaaagcagg
     1081 ccaccgtcaa cgtggcgtct ttcgctggcg gcgtcatgct gctcctcggc ttcgcgctct
     1141 tcctgatcat ccgcgtcgtg ctcaagccta tcgtcaccta cgtcgacgcg ttcaagtcgg
     1201 cgtcgaccgg cgacctgacc gtcagggcga ccgtcagggc cagggacgag ataggccgga
     1261 tagcggccta cttcaacgac ttcatagcga ctctcgagca catggtcggc gaggtcaaga
     1321 gcgttaccca gaccaccgag aacatttcct tggacctagc cgccagttcg gaggagaccg
     1381 ccgccagcct gcacgagata cgcatcaaca ccaagggcat gaagaacaag atcgtccacc
     1441 tcgacggcga ggtctcctcg tcgtccaagt cggccgagga cgtcgatagc tacctgtcca
     1501 ggctgtccga gctgatagcc gaccaggcct ccgccatcaa ccagtcgagc gcgtcgatcg
     1561 agcagatgtc cgcgagcatc aataacatag cgatggcggc cgaggagaag ctcagggtgg
     1621 ccaacgagct cgagagcacc gcgctcgaag gccagagcga gatggaggag accgagcaga
     1681 cgatcaagaa ggtctccgac agcgcctccg tcatcatgga gatgatccag atcatccagg
     1741 acatagcgag caagacgaac ctgctcgcca tgaacgccgc gatcgaggcg gcccacgccg
     1801 gcgatttcgg caaaggcttc gcggtcgtcg ccgacgagat acgcaacctg gcggagtcga
     1861 gcgccgagag cgccaagcag attacccagt cgctcggcga ggtgaccgac tacatagccg
//
LOCUS       DAOPHV010000545         1919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_85326_length_1919_cov_3.681867, whole genome shotgun sequence.
ACCESSION   DAOPHV010000545 DAOPHV010000000
VERSION     DAOPHV010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85326_length_1919_cov_3.681867"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PLQ29_11895"
     CDS             <1..768
                     /locus_tag="PLQ29_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87388.1"
                     /translation="LGWYDPPAPAGRRAYGALGVSVGGPAWAAAGAAAASLGLPGRDA
                     AAGRAEARLSVAGYRLELVASCADRFWVDVDGGQAPFLRADLSLRRSRSGLAWALGWR
                     YLRPDAYTPEGTAVSMRASCDLAAWLGLASVRARLSLADSAEAGSSVVELDARYRPAP
                     APWLSLRSSWRVVGGASERFDLGAAISAGSRWPLELESGLRFVPEGRLYKAGLSLGVS
                     GDRASVRLGIGSDGWIPEGASWVEGLEYTVRLSARFP"
     gene            830..1426
                     /gene="pncA"
                     /locus_tag="PLQ29_11900"
     CDS             830..1426
                     /gene="pncA"
                     /locus_tag="PLQ29_11900"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HPG87389.1"
                     /translation="MRIDRSRTAFLAIDIQNDFCPGGALAVEGGNEIVPLVNELAAMF
                     ELCVATQDWHPAGHISFASVGPGGGPWPDHCVQGTRGAELHSALRQDPYRLILRKGYR
                     PDLDSYSAFYENDRSTPTGLDGYLRALGASAVVVAGLALDYCVYYSALDAARLGYSVV
                     VVSDATRAVGSPEGSVDAALADLRSRGVAIIDSLEVFR"
     gene            1423..>1919
                     /locus_tag="PLQ29_11905"
     CDS             1423..>1919
                     /locus_tag="PLQ29_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HPG87390.1"
                     /translation="MNSSALFTDFYELTMAQGYWKHGLDTSAVFDVFFRRQPFKGGFS
                     VFAGLEPLLEALEVFRFSQDDLAWLSAQPGFDPAFVAYLADFRFSGDIYAMPEGELAF
                     PQEPLLRVHANLIEAQIIEGLVLNTINFQSLIATKSARVALAAKGKKVMEFGLRRAQG
                     FDGAMS"
BASE COUNT          245 a          677 c          662 g          335 t
ORIGIN      
        1 ctcggctggt acgacccgcc ggccccggcc gggcgacggg cctacggagc gctcggcgtc
       61 tccgtaggcg ggcctgcctg ggcggccgcc ggggcggctg cggccagcct cggcttgccg
      121 ggccgagacg cggcggccgg ccgcgccgag gcgcgcctgt cggtcgccgg gtaccgcctc
      181 gagctagtcg cctcctgcgc cgaccggttc tgggtcgacg tcgacggcgg tcaggcaccc
      241 ttcctgaggg ccgacctgag cctgcgccgc tcgaggtccg gcctggcctg ggccctgggc
      301 tggcgctacc tccgcccgga tgcctatacg cccgaaggta cggccgtctc catgcgagcc
      361 tcctgcgact tggccgcgtg gctgggcctg gcgagcgttc gggcgcgcct gagcctggcc
      421 gactcggcgg aggctggctc gagcgtcgtc gagctcgacg cccgctaccg tccggctccg
      481 gcgccgtggc tatcgctgcg cagttcctgg cgcgtggtag gcggcgcctc ggagcgcttc
      541 gacctgggag ccgccatatc ggcaggaagt cgctggcccc ttgagctgga gtccggcctg
      601 cgcttcgtcc ccgaagggcg cttgtacaag gccggcctgt ccttgggcgt ctccggagac
      661 cgcgcgtcgg tgcgcctcgg catcggcagc gacgggtgga tccccgaggg cgcgagctgg
      721 gtcgagggcc tcgagtatac cgtacgcctg tccgccaggt tcccgtagcg cgcgccgtct
      781 ttaccggctc gtcattatcc gttactcttg tgccgcatgg gagggaccga tgcgtatcga
      841 cagatcgagg acggcgttcc tggccatcga tatccagaac gatttctgcc ccggcggcgc
      901 cttggcggtc gagggcggga acgagatcgt gcccctcgtc aacgagctgg ccgccatgtt
      961 cgagctatgc gtggccacgc aggactggca cccggccggt cacatctcct tcgcgtcggt
     1021 cgggccgggc ggcgggccct ggcccgacca ctgcgtccag ggcacgcgag gcgcggagct
     1081 acactccgcg ctgcggcagg atccctaccg gctcatactc cgcaagggct acaggccgga
     1141 cctggacagc tactcggctt tctacgagaa cgaccgatcc accccgaccg ggctcgacgg
     1201 ctacctgcgc gccctcggcg cctcggccgt cgtcgtcgcg gggctggccc tcgactactg
     1261 cgtatactac agcgccctcg acgctgcccg tctcggctac tcggtcgtcg tcgtctccga
     1321 cgccaccagg gccgtcggct cgccggaggg ttccgtcgac gccgcgctcg ctgacctcag
     1381 gtccaggggc gtggcgatca tagattcctt ggaggttttc cgttgaactc gagcgcccta
     1441 ttcaccgatt tctatgagct gacgatggcc cagggctatt ggaagcacgg cctggatacc
     1501 agcgccgtct tcgacgtctt cttccggcga cagcccttca agggcggctt ctcggtgttc
     1561 gccggcctgg agccgctcct cgaggcgctc gaggtcttca ggttctccca ggacgacctg
     1621 gcctggctgt cggcgcagcc tggcttcgac ccggccttcg tcgcgtacct ggcggacttc
     1681 aggttctccg gagacatcta cgcgatgccc gagggcgagc tcgccttccc gcaggagccg
     1741 ctcctgcgcg tccatgccaa tctgatagag gcgcagatca tcgagggcct ggtgctcaat
     1801 acgatcaatt tccagagcct catcgcgacg aaaagcgcca gggtcgccct ggcggccaag
     1861 ggcaagaagg tgatggagtt cgggctcagg cgggcccagg gcttcgacgg agcgatgtc
//
LOCUS       DAOPHV010000546         1915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_85569_length_1915_cov_3.060753, whole genome shotgun sequence.
ACCESSION   DAOPHV010000546 DAOPHV010000000
VERSION     DAOPHV010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85569_length_1915_cov_3.060753"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            461..1027
                     /locus_tag="PLQ29_11910"
     CDS             461..1027
                     /locus_tag="PLQ29_11910"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HPG87391.1"
                     /translation="MCVLSKESLRFQYTLEQGYDSSCGMSVVATALDLYWGVPTDEDE
                     LIETALGEKLEDGDYTVSLADMAGAFESRGVAARAFRLDWDGLVGMAAKGYAPIVVHY
                     DEPEKHFALLLGFKDGRAVTVDPARGLESLSREAFEARYSGTAMALASRTLEADAVLV
                     AEAIETAAGRQRRLEEAAAMTAVSGRRW"
     gene            1021..1869
                     /locus_tag="PLQ29_11915"
     CDS             1021..1869
                     /locus_tag="PLQ29_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87392.1"
                     /translation="MVASARLAVLAALSLACVSPTTAQVSEHQRIGVERKTDWELGFE
                     LGGAVTGDAYAAVDYLSVDGYASVEALLARTWTASLYAPVSSRLAVGDGVRRAAAAGL
                     GDLELSAGWTGRRADSRLSAALRVTAPTGEWNGYAATVGVLTPGSGRWSVGATLSAAM
                     LLDPVALGASFAYDVGLPRAERFAMTWRPGDMSLSLSATEVLNDRVGYTVKAAQSASL
                     PELDGGEPSLDSFAYAARASIELWYSDGDAQARFGVSKSLTDPDSPALLYAALSYTLR
                     SERDDA"
BASE COUNT          342 a          542 c          611 g          420 t
ORIGIN      
        1 agaccgttgg gctctatatt cctaatagct ccatgaggtt gaaggattat gtagttgcat
       61 ctttgcttat tcttcttctt ctgttgcctc ttagaagaac gacattcgct ttgttcgaat
      121 atgatattac gtatataaca atttccatag tgatctattg tatctctgcc ataactgaag
      181 aagtagcatt cagattgtca tttgtaaaga acttcttgcc ggaaaatagg ctagaaagca
      241 ttgttgacaa aggaccgagt tttctagatt ttgcactgat cgcaggaatt agcttgatat
      301 ttgcatttgc tcactttgaa ttctctttta aggtcattta tacacgagtc tttttttcag
      361 tcattagcat ttttatttta gctattactc gtaaggtgtc agtcttaata ataatacatt
      421 ttataaataa tatactaaca tgagattagc ctttattttt atgtgtgttt tatccaaaga
      481 atccctccgc ttccaataca ccctcgagca gggctacgac agctcctgcg gcatgagcgt
      541 cgtcgcgacg gccctggatc tctattgggg cgtgcctacg gacgaggacg agctgataga
      601 gacggcgctc ggcgagaagc tcgaggacgg cgactacacg gtgagcctcg ccgacatggc
      661 tggggccttc gagtcgcggg gcgtggcggc gcgggccttc aggctcgact gggacgggct
      721 ggtcggcatg gcggccaagg gctacgcgcc catcgtggtg cactacgacg agcccgagaa
      781 gcatttcgcg ctcctcctgg gcttcaagga cggacgggcc gttacggtgg acccggctcg
      841 cgggctcgag tcgctgagcc gcgaggcctt cgaggcgcgc tactccggga cggccatggc
      901 gctcgcgtcg aggacgctcg aagcggacgc ggtcttggtc gccgaggcta tcgagacggc
      961 cgctggaagg caacgccgcc tcgaggaagc ggccgcgatg acggctgtat cggggcgacg
     1021 atggtagcct cagcgagact cgccgtactc gcggcgctgt cgctggcgtg cgtatctccg
     1081 acgacggctc aggtctcgga gcatcaacgc atcggcgtgg aacgcaagac agactgggag
     1141 ctaggattcg agctcggcgg ggccgtgacc ggcgacgcgt acgcggcggt agactacttg
     1201 tcggtcgacg gctacgcgtc ggtcgaggcc ctgctcgcca ggacctggac ggcgtctctg
     1261 tacgcgccgg tatcgagccg cttggccgtg ggcgacggcg tcaggcgggc cgcggcggcg
     1321 gggctcggcg acctggagct gtcagccgga tggaccggca ggcgagcgga ctcgcgcctt
     1381 tcggccgcgc tgcgggtgac ggcgccgacc ggcgagtgga acggctacgc cgcgactgta
     1441 ggcgtcctga cgccggggtc cggacgatgg tccgtcggcg cgacgctgtc cgcggccatg
     1501 ctgctcgacc ccgtcgcgct cggagcctcg ttcgcgtacg acgtgggctt gccgcgggcc
     1561 gaacgcttcg cgatgacgtg gcggccgggt gatatgtcgc tttcgctgag cgccaccgag
     1621 gtcctgaacg accgcgtcgg ttatacggtc aaggccgctc agagcgcgtc gctaccggaa
     1681 ctggacggcg gggaaccgag tctcgactcc ttcgcgtacg cggcgcgcgc ctcgatcgag
     1741 ctgtggtact ccgacggcga cgctcaggcg cgcttcggcg tatcgaagag cctgactgac
     1801 cctgactctc cggcgctgct ctacgcggcg ctgtcatata ccctgcgatc ggagcgcgac
     1861 gatgcataga ttcgtacgga ctatgaccct tgtcctgttg gctctcggcg ccgcc
//
LOCUS       DAOPHV010000547         1892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_87089_length_1892_cov_3.688623, whole genome shotgun sequence.
ACCESSION   DAOPHV010000547 DAOPHV010000000
VERSION     DAOPHV010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87089_length_1892_cov_3.688623"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="PLQ29_11920"
     CDS             <1..1446
                     /locus_tag="PLQ29_11920"
                     /inference="COORDINATES: protein motif:HMM:NF019880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HPG87393.1"
                     /translation="LYERLAGTFRHFIVADSRGAEALAYARRRGLPDEVIASFRLGYA
                     PAERSWLYRFLRGKSYSPEFLARSGLFSKKYPETAIFSDRLMFPIADARGRVVAFGGR
                     LLHGDGPKYINSPETGIFKKHDTLFALPEAAQAMRTSSTAILCEGYMDAIAFHAAGVA
                     NAVAPLGTSFTEPQAALLKRYASTVVLCFDTDGAGIAAAERAIGIAESKGLGVRVLRL
                     EGAKDPAEILEKNGPERLKKNAVSTITSEDFILENAASRLRAGDSGSKTEAFEYLFPF
                     LANLSSDIRRDSLMVAASSRLGADPASVRSDFIRFLDKGSIRRHVQAPVPVASDDFAP
                     SADAELLAAIIANAELFEAARSEIGPADFDDESLKDAFIVMEECYRSGDQQVATVVER
                     LTSAGLKEFILQRISDGAYSINPDRFVLDGVRRVKLRALEARKQRVVSRIREYDAERD
                     GDELSLNDLLYEKMYLDGELTRIKEERHGRP"
     gene            1433..>1892
                     /locus_tag="PLQ29_11925"
     CDS             1433..>1892
                     /locus_tag="PLQ29_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HPG87394.1"
                     /translation="MGDLELDPAIAKLLEYAKAKKTISFDELSDFLPEDYLNSDKIDG
                     VLALLESNNVQLEEEDLQMEEPAEPKEHEAETEPEASSTKEKKRLVYNDKESAVDDPI
                     RLYLREIGKENLLTAEQEVELSKQMEEGENIIKGIIKQSGVLISEFHAIAV"
BASE COUNT          407 a          559 c          583 g          343 t
ORIGIN      
        1 ctgtacgagc gcctcgccgg caccttcagg catttcatcg tagccgactc gcgcggcgcc
       61 gaggcgctcg cgtacgctcg gcgcaggggt ctgcccgacg aggtgatcgc ctcgttccgg
      121 ctcggctacg caccggccga acgatcctgg ctctatcgct tcctgcgcgg caagtcgtat
      181 tcgcccgaat tcctggctcg ctccggcctc ttctcgaaaa agtacccgga gacggccatc
      241 ttctccgacc gcctgatgtt cccgatagcg gacgccaggg gccgggtcgt cgccttcgga
      301 ggcagactgc tgcacggaga cggccccaag tacatcaact cgccggagac gggtatcttc
      361 aagaagcacg acacgctgtt cgccctgccg gaggccgcgc aggcgatgag gaccagctcg
      421 acggccatac tatgcgaggg gtatatggac gccatagcgt tccacgcggc aggggtcgcc
      481 aacgccgtag ccccgctcgg gacgtcgttc accgagccac aggccgccct gctcaagcgc
      541 tacgcgtcga ccgtcgtcct ctgcttcgat accgacggcg ccgggatcgc cgccgccgag
      601 agggctatcg gtatcgcgga gtccaagggc ctgggcgtcc gggtgctcag gctcgagggt
      661 gccaaggatc ccgccgagat actggaaaaa aatggtcctg aacgattgaa aaagaatgcc
      721 gtttccacta taacttccga agattttata ttggaaaatg ccgcttctcg gcttcgagcg
      781 ggtgattcgg ggtctaaaac ggaggcgttc gaatatcttt tcccatttct ggcgaatcta
      841 tcgtctgata tacggcgaga ctcgctcatg gtggccgcgt cctctcgttt gggcgcagat
      901 cccgcttcag tcaggagcga tttcatacga ttcttggata aggggtcgat acggagacac
      961 gtccaggctc ccgtccctgt agcctcggac gattttgccc cgagcgccga cgccgagctg
     1021 ttggccgcca tcatagccaa cgcggagctg ttcgaggccg cgcgctcgga gataggcccg
     1081 gcggatttcg acgatgagtc gctcaaggac gcgttcatcg tgatggagga gtgctatagg
     1141 agcggcgacc aacaggtagc caccgtcgtc gagcgcctga cgagcgcggg gctcaaggag
     1201 ttcatactgc agaggatatc cgacggggcg tactcgatca atcccgatcg cttcgtactt
     1261 gacggagtcc ggcgagtgaa gcttagggcg ctcgaggccc ggaaacagcg cgtcgtctcg
     1321 cgtatccgcg agtacgacgc cgagcgggac ggcgacgagc tgtccctcaa cgaccttctc
     1381 tatgaaaaaa tgtatctaga tggagaatta accaggataa aggaagagcg gcatgggcga
     1441 ccttgaactc gatccggcga tagcgaaact actcgaatac gcgaaggcca agaagacgat
     1501 cagcttcgac gagctgtcgg acttcctgcc cgaggattac ctgaattcgg ataagataga
     1561 cggcgtgctg gcgcttctcg agagcaataa tgtgcagctc gaggaagaag acctccagat
     1621 ggaggagccg gcggagccca aggagcatga ggcggagacc gagcccgaag cctcctcgac
     1681 caaggagaag aagcgcctcg tctacaacga caaggagagc gccgtcgacg atcctatccg
     1741 gctgtacctc cgcgagatag gcaaggaaaa cctcctgacc gccgaacagg aggtcgagct
     1801 atccaagcag atggaggaag gcgagaacat catcaagggg atcatcaaac aaagtggtgt
     1861 cttgatttcc gagttccacg ccattgccgt ca
//
LOCUS       DAOPHV010000548         1883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_87667_length_1883_cov_4.193107, whole genome shotgun sequence.
ACCESSION   DAOPHV010000548 DAOPHV010000000
VERSION     DAOPHV010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87667_length_1883_cov_4.193107"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="PLQ29_11930"
     CDS             <1..1073
                     /locus_tag="PLQ29_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG87395.1"
                     /translation="STLRRELDAYTAELSSQRDKLSSLLREVERAADETSSVAQTLEA
                     EVAEVADEARRSAASANDIGVSASAQRSALDGASDSISSLLRSIASVQEELSLEAASI
                     ERTARDTGGMIDGMTRVGASIEGTATFASSLDRLTAEATGSMNKLAASMDKVTKASDE
                     IRSVVGAVGELADRTNLLAMNASIEAAHAGAAGRGFAVVAHEIKALAQASAERAASIG
                     DMVARIETAVGEGVESNRTVGESLARIAEGAASTSASVTEAALEARRQVGSGARVAEE
                     ARALAASASRMSEEAETESRLSERASADMKALEAGHAAIADSASLVIERNAGLAEKAQ
                     ALKVLAARARAAADSLVNAMSR"
     gene            1070..>1883
                     /locus_tag="PLQ29_11935"
     CDS             1070..>1883
                     /locus_tag="PLQ29_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPG87396.1"
                     /translation="MSAAESALDESGSDAPSTDEVAAFKAAVLRLYEREGRDLPWRRR
                     VGPWAVLVSEVMLQQTQVSRVEPRYRSWMERFPDAAALARAELAEVYEAWRGLGYNSR
                     ALRLRETARVCDERYGGVPPADERLLLELPGVGRYTARAVLAFAYDRPSAFLETNIRA
                     ALIFHFRPGRDRVGDRELEALAEAVLDRDDPRTWYYALMDYGAWLKKREPNPTRRAAA
                     YSTQSRFKGSVREARGAILRGLAGSGTIALTEIAAEDGIEYARLERAACAGSA"
BASE COUNT          318 a          621 c          679 g          265 t
ORIGIN      
        1 cctcgacgct acgccgggag ctcgacgcct acaccgccga actttcgtcc cagcgggaca
       61 agctgagctc gctcctgcgc gaggtggagc gggctgccga cgagacctcg agcgtagccc
      121 agacgctcga ggcggaggtg gcggaggtgg cggacgaggc taggcgctcg gcagcctccg
      181 ctaacgacat aggcgtctcg gcgtcagctc agcgctcggc gctcgacggc gcctcagact
      241 ccatctcgag cctgctccga tcgatagcgt ctgtccagga ggagctgtcg ctcgaggccg
      301 cgagcatcga gcgcacggcc cgcgacaccg gcggcatgat agacggcatg accagggtcg
      361 gggcgtccat cgagggtacg gccaccttcg cctccagtct cgaccggctg accgccgagg
      421 caaccggatc gatgaacaag ctagcggcat cgatggacaa ggtaacgaag gcgtcagatg
      481 agatacggtc ggtagtcggg gcggtgggcg aactggccga ccggaccaac ctgctcgcta
      541 tgaacgcctc gatcgaggcc gcgcacgccg gcgccgccgg acgcggcttc gcggtggtgg
      601 cgcacgagat aaaggcgctg gcccaggcca gcgccgagcg tgcggccagc atcggtgaca
      661 tggtcgcgcg tatagaaacc gcggttggcg agggcgtcga gtcgaaccgg acggtcggcg
      721 agtcgctggc taggatagcc gaaggcgcgg cgagcacgag cgccagcgta accgaggccg
      781 ccctcgaagc caggcgacag gtgggctcgg gagcccgcgt cgccgaggaa gccagagccc
      841 tcgcggcgtc cgcgagcagg atgtccgagg aggccgagac cgagtcgcga ctatcggagc
      901 gagcctccgc cgacatgaag gcgctcgaag ccggccacgc ggcgatagcc gattcggcct
      961 cgctcgtcat cgagcggaac gctgggctcg cggagaaagc ccaggcgctc aaggttctgg
     1021 cggctcgcgc cagggctgcc gccgactcgc tcgtgaacgc aatgagtcga tgagcgcggc
     1081 cgagtcagcc ctcgacgaat caggtagcga cgcgccttcg accgacgagg tggcggcctt
     1141 taaggccgcg gtgctccgcc tgtatgagcg ggagggccgc gacctgccat ggcgccgccg
     1201 cgtcgggccg tgggcagtac tcgtttcgga ggtgatgcta cagcagaccc aagtctcgcg
     1261 cgtcgagcct cgctatcgtt cctggatgga acgtttcccg gacgcggccg ccctggcgcg
     1321 cgccgagctc gccgaggtct acgaggcgtg gcgtgggctg ggctacaact cgcgggccct
     1381 acgcctcagg gagactgcgc gggtttgcga cgagcgatac ggcggcgttc ccccggccga
     1441 cgagcgcttg ctcctcgagc tacccggcgt cggccgctac acggccaggg ccgtgttggc
     1501 cttcgcctac gatcgaccct cggcgttcct cgagacgaac atccgggccg ccctcatctt
     1561 tcacttccgc cccggcagag accgagtcgg cgatcgagag ctcgaggccc tggccgaggc
     1621 agtgctcgac cgtgacgatc cgaggacctg gtactacgcg ctgatggact acggcgcctg
     1681 gcttaagaaa cgggagccca acccgacgcg gagggccgcc gcttattcga cgcagtcgcg
     1741 tttcaaaggt tcggtcaggg aggccagggg ggctatccta cgtggactcg caggtagcgg
     1801 gacgatcgcg ctgactgaaa tagccgccga ggacggcatc gagtacgctc ggctcgagcg
     1861 ggccgcctgc gccggctcgg cgt
//
LOCUS       DAOPHV010000549         1881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_87801_length_1881_cov_3.474808, whole genome shotgun sequence.
ACCESSION   DAOPHV010000549 DAOPHV010000000
VERSION     DAOPHV010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87801_length_1881_cov_3.474808"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PLQ29_11940"
     CDS             complement(<1..645)
                     /locus_tag="PLQ29_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPG87397.1"
                     /translation="MPEPPPNALPDSFHPLVRSWFTERYGEPTPVQAAAWPVIASGGH
                     VLASAPTGSGKTLTAFLGALSRFATGEYPAEGLSVLYVSPLKALNEDVRLNLLEPARE
                     LSAWFRDRGQAFPRLRIASRSGDTSQSERRGMIARPPAILCVTPESLAIMLASRSGLA
                     MLGSVRLLILDELHAVLGSKRGAALACSVGRLALFAGEFQRVALSATVEPFDVAA"
     gene            798..1277
                     /locus_tag="PLQ29_11945"
     CDS             798..1277
                     /locus_tag="PLQ29_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HPG87398.1"
                     /translation="MDANTLLVTIALSFLPISELRGAIPFAVARGTPLLEATAIAVAC
                     NVLVGPVAFVFLETFHRLLYRWAFYARVFDRFVAKARAKVKEKVEKYGYLGLMLFVAV
                     PLPVTGAWTGALGAWILGMGRRKAMLAVAAGVLVSGMIVSLIVGFGVEALSFLLKRV"
     gene            1285..>1881
                     /locus_tag="PLQ29_11950"
     CDS             1285..>1881
                     /locus_tag="PLQ29_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPG87399.1"
                     /translation="MHLDYEAELNPEQYAAVTAIEGPVLIIAGAGSGKTRVITYRAAY
                     MLEKGVPQSAILALTFTNKAAKEMSERVRSVTGKKLQNLTVSTFHSFGVKILRKEIHR
                     LGWRDNFSIYDEVDRTQLIKDCAREVGFKADAFDAQKAGLVFSDVKTGRKNWEGVDDG
                     YEKVYDEYQAALKAYNAVDFDDLIGLPIAVFERFEDARE"
BASE COUNT          307 a          621 c          650 g          303 t
ORIGIN      
        1 ggccgccacg tcgaagggct cgaccgtagc cgatagggcg acgcgctgga actcgcctgc
       61 gaacagggcg agcctgccga cggagcaggc gagcgcggcg ccgcgcttgg agcctagcac
      121 cgcgtggagc tcgtcgagta tcaggagccg gaccgagccg agcatggcca gcccggagcg
      181 cgaggcgagc atgatggcca ggctctcggg ggtcacgcag agtatggccg gcggcctggc
      241 tatcatgccg cggcgctcgg actggctcgt gtcgcccgag cgcgaggcta tcctgagccg
      301 cgggaaggcc tgcccccgat cgcggaacca ggcggacagc tccctggccg gctcgagcag
      361 gttgagccgg acgtcctcgt tgagcgcctt gagcggcgac acgtacagca cggacaggcc
      421 ctcggccggg tactcgccag tggcgaagcg tgacagggcg cccaggaagg ccgtcagggt
      481 cttgcccgag ccggtcggcg cggaggcgag gacgtgccct cccgaggcta tgaccggcca
      541 ggccgcggcc tgtaccggcg tcggctcgcc gtagcgctcg gtgaaccagg agcgtacgag
      601 cgggtggaac gagtcgggaa gggcgttcgg cggcggttcg ggcatgggac gatagtacgc
      661 gatcgtccgg gtacgctcaa gtatcgtcgg actaacggcg ccgacggtac tccggcgccg
      721 acggtactcc ggcgccgacg gtactccggc gcccatccgc ttgtccggcg ccacggcttc
      781 cggctatact ccggccaatg gacgccaaca cgctgctcgt taccatagcc ctgtccttct
      841 tgccgatctc ggagctacgc ggggccatac ccttcgccgt ggcccgcggg acgccgctcc
      901 tcgaggccac cgcgatcgcg gtggcctgca atgtcctcgt cggcccggtg gccttcgtct
      961 tcctcgagac cttccatcgg cttctgtatc gatgggcctt ctacgcccgc gtgttcgacc
     1021 gcttcgtggc caaggccagg gccaaggtca aggagaaggt cgagaagtac ggctaccttg
     1081 gcctcatgct gttcgtggcc gtgccgctgc ccgttacggg cgcctggacc ggcgcgctcg
     1141 gcgcctggat tctcggtatg ggtcgccgca aggcgatgct tgcggtggcg gccggcgtgc
     1201 tcgtctctgg catgatcgta tcgctgatcg tcggcttcgg cgtcgaggcc ctgtccttcc
     1261 tccttaaacg ggtctaggag gcgcatgcac ctcgactacg aggcggagct caacccggag
     1321 cagtacgccg ccgtcacggc gatagagggg cccgtgctga tcatcgcggg cgccggctcc
     1381 ggcaagaccc gcgtgatcac ctatcgcgcc gcgtacatgc tcgagaaggg cgtgccgcaa
     1441 tccgccatcc tcgcgctgac cttcacgaac aaagccgcca aggagatgtc ggaacgcgtc
     1501 cgctcggtca cgggcaagaa gctccagaat ctgaccgtca gcaccttcca ctcgttcggc
     1561 gtcaagatcc tgcgcaagga gatccaccgc ttggggtggc gcgacaactt ctcgatctac
     1621 gacgaggtgg ataggaccca gcttatcaag gactgcgccc gcgaggttgg cttcaaggcc
     1681 gacgccttcg acgcgcagaa ggccggcctg gtgttttccg atgtcaagac cgggcgcaag
     1741 aactgggaag gggtcgacga cggctacgag aaggtctacg acgagtacca ggccgccctc
     1801 aaggcctata acgcggtcga cttcgacgac ctgatcggcc tgccgatcgc ggtgttcgag
     1861 cggttcgagg acgcacgcga a
//
LOCUS       DAOPHV010000550         1881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_87810_length_1881_cov_3.282585, whole genome shotgun sequence.
ACCESSION   DAOPHV010000550 DAOPHV010000000
VERSION     DAOPHV010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87810_length_1881_cov_3.282585"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /gene="ftsA"
                     /locus_tag="PLQ29_11955"
     CDS             complement(<1..1167)
                     /gene="ftsA"
                     /locus_tag="PLQ29_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPG87400.1"
                     /translation="MGLDIGTSKTCAVIGEYNESGTLEITGVGVAPSTGLRKGVVVNI
                     EATLQSVTKAVEAAELMSGREARDCFVGVAGANVEGINSRGVVAVTGKGREICQSDVD
                     RVIEAARAIVIPMDREILHVIPQTFIVDDQKGIRDPLDMIGVRLEAEVHIITGSVTTA
                     QNLVRCVNRAGFKADGLILQSLAASRAVLTADEKELGCLLVDLGGGTTDVLMYSEGAP
                     YFSASVGAGGSQVTNDLSIILNVPMDAAERIKRESGCCYLPHVELDESIVVPGVGGRP
                     PQEIAKAKVASVIQPRMEEIFRLAREKVEQPGFWRHVKGGVVLTGGGALMPGAAELAY
                     EVFGVPVRIGAPASLGGLVDAYRNPAYSTAVGLVLLGSERLGAPEPDARGKASGA"
     gene            complement(1178..>1881)
                     /locus_tag="PLQ29_11960"
     CDS             complement(1178..>1881)
                     /locus_tag="PLQ29_11960"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPG87401.1"
                     /translation="AAFALPRTEVRGLTVSGAVTIGERELYAWSGLSERQHWFSVDRG
                     AVAACLSAHPRVASVSVETRFPDTVAVAVVERRPIAVVYARGDSGRTEAHCVDSEGVV
                     FAAASMYPGSSALPVLSGVEIRGLSYGIKLGFPFSELLSSLAEVAEASPALVSAISEL
                     RVVSKPGLPAELLLYPTDYRVPVRMRPVLNAGLLKSMMLVLDVVEGEGLSPGIRELDL
                     RTDTFVYRTKEAVSG"
BASE COUNT          315 a          681 c          602 g          283 t
ORIGIN      
        1 ggcgccggag gccttgcccc tcgcgtccgg ctcgggcgcg ccgagacgct cggaacccag
       61 cagcacgagg ccgaccgcgg tcgagtacgc cgggttgcgg tacgcgtcga cgagcccgcc
      121 gagcgacgcc ggcgcgccga tcctgaccgg caccccgaac acctcgtacg ccagctccgc
      181 cgccccgggc atcagcgcgc cgccgccggt cagcacgacg ccgcccttga cgtgtcgcca
      241 gaagccgggt tgctcgacct tctcgcgagc cagcctgaat atctcctcca tgcgcggctg
      301 gatcacgctg gcaaccttgg ccttcgctat ctcctggggc ggcctgccgc cgactcccgg
      361 cacgacgatg ctctcgtcga gctcgacgtg cggcaggtag caacagcctg actcgcgctt
      421 gatgcgctcg gcggcgtcca tcggcacatt caggatgatc gacaggtcgt tcgtgacctg
      481 cgagccgccg gctcccaccg acgcgctgaa gtacggggcg ccctccgagt acataagtac
      541 gtccgtggtg ccgccgccga ggtcgacgag caggcagccc agctccttct cgtcggccgt
      601 aagcacggcc cgcgacgccg cgaggctctg gagtatcagc ccatcggcct tgaagccggc
      661 gcggttgacg caacggacca ggttctgggc cgtcgtcacc gaaccggtga tgatgtgcac
      721 ctcggcctcg agcctgacgc ctatcatgtc caacggatcg cgtatgccct tctggtcgtc
      781 gacgatgaag gtctggggga tcacgtggag gatctcccta tccatcggaa tgacgatcgc
      841 cctggcagcc tcgatcaccc tgtccacgtc ggactggcag atctcgcgcc ccttgccggt
      901 caccgcgacc acgccgcgcg aattgatgcc ctcgacattc gcaccggcga cgccgacgaa
      961 gcagtcgcgc gcctcgcgcc cggacatcag ctcggccgcc tcgacggcct tcgtgaccga
     1021 ctgcagggtc gcttcgatat tgacgacgac gcccttgcgc aggccggtgc tcggggcgac
     1081 cccgacgccc gtgatctcga gagtcccgga ttcgttatat tcgccgatca ccgcgcaggt
     1141 cttggacgtg ccgatatcga gccccacgat cagattgtca cccagagaca gcctcctttg
     1201 tccggtacac gaacgtgtcg gtccgcaggt ccagctcgcg gatgccgggg gacagtccct
     1261 ccccctcgac gacgtcaagg acgagcatca tagacttcag tagcccggcg ttcaatacgg
     1321 gccgcatcct gaccggtacc cggtagtccg tcgggtacag gagtagctcg gccgggagcc
     1381 cgggcttcga gacgacgcgc agctcggaga ttgccgatac cagggcgggg ctcgcctcgg
     1441 cgacctcggc gagcgacgac aggagctcgg agaacgggaa accgagcttg atcccatagc
     1501 tcaggccccg tatctcgacg ccggacagca ccggcagggc cgacgagccc ggatacatcg
     1561 aggccgccgc gaacacgacg ccttcggaat cgacgcagtg cgcctcggtg cggccggaat
     1621 cgccgcgggc gtacaccacc gctataggcc tgcgctcgac caccgcgacg gctaccgtat
     1681 cgggaaaacg cgtctcgacc gagacgctgg ccacgcgcgg gtgggccgac aggcaggcgg
     1741 ccacggcccc gcggtcgacc gagaaccaat gctggcgctc cgaaaggccg gaccacgcgt
     1801 acagctcgcg ctcccctatc gtgacggccc cggatacggt gaggcccctc acctcggtgc
     1861 gtggtaacgc gaaggccgct a
//
LOCUS       DAOPHV010000551         1876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_88130_length_1876_cov_4.075233, whole genome shotgun sequence.
ACCESSION   DAOPHV010000551 DAOPHV010000000
VERSION     DAOPHV010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88130_length_1876_cov_4.075233"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PLQ29_11965"
     CDS             <1..929
                     /locus_tag="PLQ29_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPG87402.1"
                     /translation="SLAGLFLREVKRRADAASGVACAAGVVEEAVVARYRAACAYAGF
                     EEVHFVPEPVAAAAGMAGELSGTVLVFDFGGGTLDVAVAEPGADGVRILSSAGRDLGG
                     YLLNEDVSRARITRHFGSEGKIRTMKGSYLDMPRWITDQVASFYALPLSDLAATRRLI
                     KELIYDARPIDKPRLRGLAEFLDRNLTFELFDAIDAAKIALSVGQESEIAFALPPHVS
                     FVERLTRAEFEGVIGRRVDEARALVLEALERSGRRPADVSTVIRVGGSSRVPAFAAML
                     ESLFPGRVREGAVFTSIASGLLAAHDSGLSAA"
     gene            complement(926..>1876)
                     /locus_tag="PLQ29_11970"
     CDS             complement(926..>1876)
                     /locus_tag="PLQ29_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87403.1"
                     /translation="GYVYDRTLSAGAWDFARERLLGSAERLATLVAEKPRHYRFPYSL
                     VRPLVSGDRIYERFLDARSNLERPSCLGGDCAACGACQGDERAFLAAHRLGSATEADL
                     RAVEAVVAAKRRPYETWVRSTLPREAATADPAYPATLFRRDLYAALPALVDTVWTAED
                     ALLKNKTGLERLPGAWGDTWYRVLSSAPIDPDALETAGYAVGSAAPAPERLYLRVSLA
                     GASHAEAARLVAGFLDASAIPHTLRKTAAATVFAVSDKGRKKRNILEASIPASLQASV
                     QGRAIIDLACGSKYDVANLMKAAAKRGLYPELRVGLEPLP"
BASE COUNT          277 a          644 c          672 g          283 t
ORIGIN      
        1 agtccctggc cgggctgttc ctgcgcgagg tcaagcgcag ggccgacgcg gcctccggcg
       61 tagcatgcgc ggcgggcgtc gtcgaggagg ccgtcgtcgc ccgctaccgc gcggcctgcg
      121 cctacgccgg cttcgaggag gtacacttcg tgccggagcc ggtggccgcg gccgcgggca
      181 tggccgggga gctgtccggc accgtcctcg tattcgactt cggcggcggc acgctcgacg
      241 tggcggtggc cgagccggga gccgacggcg tccgaatact gtcgagcgcc ggccgggacc
      301 tcggcggcta cctcttgaac gaggatgtgt cgcgggccag gatcacgagg catttcggct
      361 ccgagggcaa gatacgtacg atgaagggca gctacctgga catgccgcgc tggatcaccg
      421 accaggtggc ctcgttctac gccctacccc tgtccgacct ggccgccacg aggcgcctga
      481 tcaaggagct gatctacgac gcgcggccta tcgacaagcc gcgtctgcgc ggcctggccg
      541 agttcctcga ccgcaacctg accttcgagc tgttcgacgc catcgacgcg gccaagatcg
      601 cgctatcggt cggccaggaa tcggagatag ccttcgccct gccgccgcac gtctcattcg
      661 tcgagcgtct gacgcgggcc gagttcgagg gcgtcatagg ccgcagggtc gacgaggcgc
      721 gcgccctggt gctcgaggcg ctcgagcgct cggggaggcg gccagccgac gtgtcgacgg
      781 tcatccgcgt cggcggctcg tcacgcgtgc cggccttcgc cgccatgctc gagtcgctgt
      841 tcccgggacg cgtccgggaa ggcgccgtgt tcacgagcat cgcttccggg cttctggccg
      901 ctcacgattc gggcctatcg gctgcctagg gtagcggttc aagccctacc cgcaactcgg
      961 ggtagagccc tcgcttggcc gcagccttca tcaagttggc tacgtcgtac ttcgagccgc
     1021 aggccaggtc gatgatggcc cggccctgaa cggaagcctg gagagaagcc ggaatggaag
     1081 cctcgagtat gttgcgcttc ttcctaccct tgtcggacac cgcgaagacc gtagcggccg
     1141 cggtcttcct gagcgtatgc gggatagccg acgcgtccag gaagcccgcg acaagcctcg
     1201 cggcctcggc gtggctggcg ccggccaggc tcacacgcag gtacaggcgc tcgggggccg
     1261 gcgccgcgga tccgacggcg tagcctgccg tctcgagcgc gtccggatcg atcggggccg
     1321 acgacaggac gcggtaccag gtatcgcccc acgcgccagg caggcgctcg agccccgtct
     1381 tgttcttgag caaggcgtcc tcggcggtcc ataccgtatc gacgagggcc ggcagcgcgg
     1441 cgtacaggtc cctacggaac aaggtcgccg gatacgccgg atcggccgtc gccgcctccc
     1501 tgggcagggt cgagcggacc caggtctcat acggccggcg cttggccgcg acgacggcct
     1561 cgacggcccg gaggtcggcc tcggtagccg agccgagcct atgagcggcc aggaaggccc
     1621 tctcgtcgcc ctggcaggcg ccgcaggccg cgcagtcgcc gccaaggcag ctcggccgct
     1681 ccaggttact cctggcgtcc aggaagcgct cgtagattcg gtcgccggag acgaggggac
     1741 gcacgagcga gtacgggaac cggtagtggc ggggcttctc ggcgaccagg gtcgccaggc
     1801 gctcagccga gccgaggagg cgttccctgg cgaagtccca ggcgccggcc gacagggtac
     1861 ggtcatagac gtagcc
//
LOCUS       DAOPHV010000552         1862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_89080_length_1862_cov_3.375761, whole genome shotgun sequence.
ACCESSION   DAOPHV010000552 DAOPHV010000000
VERSION     DAOPHV010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89080_length_1862_cov_3.375761"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..983)
                     /locus_tag="PLQ29_11975"
     CDS             complement(219..983)
                     /locus_tag="PLQ29_11975"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPG87404.1"
                     /translation="MTTTVPYTVPECAPIRVDADGPECCLCVHGFTSCPAVYTELAER
                     LAGMGYALSAPLLPGHGTTPEDLGAVAEIEWFEAVAREYEALRGGYRTIHVVGLSLGG
                     AIASWLAASRAGEPGLGRLVLLSPAYGLANKDYYAVDYASAEDRLIPIRVRPRLGDGL
                     DAGRYQYPAMPLRAVGQLMLAAERGRASRPGITAPTALLYTAADAVVDPVRIEEAATS
                     IRSIERVQRYDGGEHNLLLGPDRLDVVERVAGFLER"
     gene            1231..1596
                     /locus_tag="PLQ29_11980"
     CDS             1231..1596
                     /locus_tag="PLQ29_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87405.1"
                     /translation="MKKLGMLLAILLVAGVCAMADEAVAAQDDALFADIDAVALSQVE
                     MEAVEGDGFGTFVIGAIVGGILYDCANDTTKELTGKTIKGHYTDAINKAVESAKNYYN
                     NYSPNYDAPGPRARARARG"
BASE COUNT          396 a          498 c          567 g          401 t
ORIGIN      
        1 gttcatgcta caagtatccg caaaacggcg acgaaatgga agactggttc ctcgaagctt
       61 gtggagcgtc gcgggacgcc gcgataggcg ggccggcgtc ttcgccgggc taccagatcc
      121 tgtagcgacg gcttcttcgc cacaagcttc gaggaactag gtatcgagca cggccgacag
      181 gccgtcggat aacggcccgc caagcgatac gagcggcgct atcgctccag gaagccggct
      241 acgcgctcca cgacgtcgag gcgatcgggg ccgaggagca ggttatgctc gccgccgtcg
      301 taacgctgga cccgctcgat cgaccggatc gacgttgcgg cctcttcgat acgtaccggg
      361 tcgacgacgg cgtcggccgc ggtatacagg agcgccgtcg gggcggttat gccgggccgc
      421 gacgcccggc cgcgctcggc cgcgagcatg agctggccga cggccctgag cggcatcgcc
      481 gggtactgat agcggccggc gtccagcccg tcgccgaggc ggggccgtac ccgtatcggt
      541 atgagccggt cctcggcgct cgcgtagtcg acggcgtagt agtccttgtt ggctagcccg
      601 taagccggcg acaggaggac gagccgcccg aggcccggct cgcccgcgcg gctcgcggcc
      661 agccaggagg ctatcgcccc gccgagcgat agcccgacga cgtggatcgt ccggtagccg
      721 ccgcgcagcg cctcgtactc ccgggccacg gcctcgaacc actcgatctc ggccaccgcg
      781 ccaaggtcct cgggcgtcgt gccgtggccg ggaaggagcg gcgcgctcag cgcgtagccc
      841 atgccggcca gcctctcggc cagctccgta tagaccgccg ggcagctcgt gaaaccgtgc
      901 acgcagaggc agcactcagg gccgtcagcg tcgacgcgga taggagcgca ctcggggacg
      961 gtatagggaa cggtagtcgt catgggcgta tagtagcggg tttgaagctt atgttgaact
     1021 acgcgtatat atcgttgcct attcgcctat tagtagcctt gaagatttta gatacgactc
     1081 gattcaaata tacttgatac attgttatga gttcattctg taaataaata ttgacaaagt
     1141 tcgagagggg gggggtagca ttctatcgtc ttccgctcat ccgggcccgg cggaacggct
     1201 agataaaacc gttactaaat ggagtagcat atgaagaagc taggaatgct cctcgcgatc
     1261 ctgctggtcg ccggagtctg cgccatggcc gacgaggccg tagcggccca ggacgatgcc
     1321 ctcttcgccg atatagacgc cgtggcgctg agccaggtcg agatggaggc ggtggaggga
     1381 gatgggtttg ggacttttgt tattggtgcg atcgttggcg gcatcctata tgattgtgca
     1441 aatgatacga cgaaggagct tactggaaag acaatcaagg gccattatac cgatgctata
     1501 aacaaggctg tagaatcagc caaaaattat tataataact attcgccaaa ttatgacgct
     1561 cctggtccta gagctagggc tagggcaaga ggttagctat ttagggcggt atgcaataag
     1621 catccgccct tgttttattt gaggtcgtaa tgaaagtaat tgatttgtca atgctattgt
     1681 tggtgttatt cagtattact cttcaactag taagagaata taaaaacaaa tatattaaac
     1741 agtgcattgc gatgtttagt tgccttgtct caactaccgc ttcccagata ttctatgatt
     1801 tagttgttga ttgtaattat atcctcatca cattgttcgc gattgttatc attattaata
     1861 tt
//
LOCUS       DAOPHV010000553         1858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_89325_length_1858_cov_4.229617, whole genome shotgun sequence.
ACCESSION   DAOPHV010000553 DAOPHV010000000
VERSION     DAOPHV010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89325_length_1858_cov_4.229617"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..805)
                     /locus_tag="PLQ29_11985"
     CDS             complement(146..805)
                     /locus_tag="PLQ29_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPG87406.1"
                     /translation="MEKAIKDSYKRLIVEGGRQMLAEGLTVGTWGNLSTRDPETGLVY
                     VKPSGMKYGEITEGDVVVFDPSYEIVDGSRKPTIEYRFHIGIMNARPDVNCVIHTHPV
                     YSAVLGVLGIDLPGVCEDFVQLVGDRIVNCEYALPGTPELAANVVAALGDRNAVMIPN
                     HGTVCCGPDWDTVKKVCFVVEKAAQIYVLARSIGEPRLIGQADVEAMQDFARHHYGQG
                     R"
     gene            complement(857..>1858)
                     /locus_tag="PLQ29_11990"
     CDS             complement(857..>1858)
                     /locus_tag="PLQ29_11990"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPG87407.1"
                     /translation="DGSTLSLAEMIRGGVTCFADMYFFMDEVARATERAGLRARLSRG
                     LAFNAPGDEAKLDESRAFHAAWNGAAGGRILVDVAPHAVYTCPPAFIERASDLASELG
                     CMIHVHLSESRKEVADCLAAYGKSPVAHVRDLGLFRNRTYAAHCVHVDDADIATLAEG
                     GVAVVNNPSSNLKLGNGFAPVGRMLAAGVNVALGTDGQASNNNLNMFEEMHVAALVNK
                     GLEEDPTALSAYTTLRMATIGGARALGLDEEIGSLRVGKKADLIILDASAPHFYPRNS
                     VTAALVYAAQGQDVRTVFCDGEPLMLDGAMTTIDEAEACAKAQESASAMTGGAGFTP"
BASE COUNT          292 a          623 c          612 g          331 t
ORIGIN      
        1 ggaaccaaca gaaggtcgtc gcggccctgg cctcgcgcct atcggacgaa ggctacctgt
       61 atctcggcca ctccgagacg atcagcaacc tcgacctgcc actcaggacg ataggccacg
      121 cggtttacca gctcaggtga tacggtcagc ggccctggcc gtagtggtgc ctggcgaagt
      181 cctgcatggc ttccacgtcg gcctggccta tgagcctggg ctcgcctatg ctcctggcca
      241 gtacgtagat ctgggcagcc ttctccacca cgaagcatac cttcttgacc gtgtcccagt
      301 ccggcccgca acagacggtg ccatggttcg ggatcatcac cgcgttgcgg tcgccgaggg
      361 ccgcgacgac gttggcggcc agctcgggag tgccgggcag ggcgtactcg cagttgacga
      421 tccggtcccc gacgagctgg acgaaatcct cgcagacgcc gggcaggtcg atgcccagga
      481 cgccgagcac cgcggaatac accgggtggg tatggatcac gcagttgacg tccggccggg
      541 cgttcatgat gccgatatgg aagcggtact cgatggtagg cttgcgtgac ccgtcgacga
      601 tctcgtacga cgggtcgaag acgacgacgt cgccctcggt gatctcaccg tacttcatgc
      661 ccgacggctt gacgtagacg agtcccgtct ccgggtcgcg cgtgctcagg ttaccccagg
      721 tgccgaccgt caggccctcg gcgagcatct gcctgccacc ctcgactatc agccgcttat
      781 acgaatcctt gatggccttc tccatgtcgt gttcctcgtc cttcccgtcg ttccgctagg
      841 ccgcgctccg cggcgctcag ggagtaaagc ccgctccgcc ggtcatcgcg gaggcgcttt
      901 cctgggcctt ggcgcaggcc tcggcctcgt ctatcgtcgt catggcgccg tcgagcatga
      961 gcggctctcc atcgcagaag acggtacgca catcctgccc ctgggccgcg tagaccagag
     1021 ccgccgtaac gctgttgcgg ggatagaaat gcggcgcaga cgcgtcgaga atgatcaggt
     1081 cggccttctt tccgacgcgc aggctaccta tctcctcgtc gaggccgagc gccctcgccc
     1141 cccctatcgt cgccatgcgt agcgtcgtat aggccgacag cgcggtcgga tcctcctcga
     1201 ggcccttgtt gacgagggcc gccacgtgca tctcctcgaa catgttcagg ttgttgttcg
     1261 acgcctggcc gtcggtgccc agcgcgacgt tgacgccggc ggcgagcatg cgcccgaccg
     1321 gagcgaagcc gttcccgagc ttgagattgc tcgacgggtt gttgacgacc gcgacgccgc
     1381 cctcggcgag cgtcgctatg tcggcgtcgt cgacatggac gcagtgggcc gcgtaggtcc
     1441 tgttcctgaa caggccgagg tcccggacgt gggcgacggg gctcttgccg taggcggcca
     1501 ggcagtcggc cacctccttg cggctctcgg acaggtgaac atggatcata cagccgagtt
     1561 cggaggccag gtcggaggcg cgctcgatga aggccggcgg gcaggtatac accgcgtggg
     1621 gagccacgtc gaccaggatg cgcccgccgg cggcgccgtt ccaggccgcg tggaaggcgc
     1681 ggctctcgtc gagcttggcc tcgtcgccgg gggcattgaa ggcgagcccg cgcgatagcc
     1741 tggcccggag tccggcgcgc tcggtcgccc tggccacctc gtccatgaag aagtacatat
     1801 cggcgaagca ggtgaccccg ccgcgaatca tctcggcgag gctgagcgtc gagccgtc
//
LOCUS       DAOPHV010000554         1857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_89397_length_1857_cov_4.184240, whole genome shotgun sequence.
ACCESSION   DAOPHV010000554 DAOPHV010000000
VERSION     DAOPHV010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89397_length_1857_cov_4.184240"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLQ29_11995"
     CDS             complement(<1..463)
                     /locus_tag="PLQ29_11995"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPG87408.1"
                     /translation="MPAGRSLRVSFRTFGCKLNQAETEALADGFSAAGAVVVGPGELA
                     DLVVFNTCTVTSKAEQKARREMRLALRRNPEAVAIVTGCYAELEPEAVAAVAPRCVVV
                     PGSRKGSVVGMASGLVAARLEGLDPLDELRRLAALAAGREADPFAFAPGDAL"
     gene            complement(469..1107)
                     /locus_tag="PLQ29_12000"
     CDS             complement(469..1107)
                     /locus_tag="PLQ29_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG87409.1"
                     /translation="MGPRPNAARAVAAALALSVSSGLYAQDRPDALKMYREGRFDDAR
                     TVCLAELAQDPLNIESYVVLVWSLLALERYADAELYANKAYTTVRRDPRIVESLGEAA
                     YYQGKNRQALDHFRDYVNLLPDGARIGTVYYLSGETYLRLERLEHADIAMRTALQYEP
                     RNARWWARLGYVRERASDWANALAAYDAALAINPSLVDAVRGRERVLARTRR"
     gene            complement(1125..1496)
                     /locus_tag="PLQ29_12005"
     CDS             complement(1125..1496)
                     /locus_tag="PLQ29_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPG87410.1"
                     /translation="MTDVHLSVVDEDTLDTILASDVEFAGSMAFKEPMMIKGRVSGSI
                     VSDSDLHIDEKAVVEADVRAGNVTVRGSLKGNIVATGRVELYASCRVEGDVTAAEVTM
                     EPGCRFNGICTMTGDSSSTQA"
     gene            complement(1526..>1857)
                     /locus_tag="PLQ29_12010"
     CDS             complement(1526..>1857)
                     /locus_tag="PLQ29_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HPG87411.1"
                     /translation="CQSAYAVRSVFDLSPGSFWPQPRVSSSVVTMEARPDAVAHSGDK
                     AFTGFTRACFSSRRKTLRNNLKAAGRPEPLIAAACEAAGVSPDARAETLTPELFSELY
                     DAFGRLA"
BASE COUNT          285 a          612 c          687 g          273 t
ORIGIN      
        1 acagcgcgtc tcccggcgcg aaggcgaagg ggtcggcctc gcggccggcg gccagggcgg
       61 ccagccgccg tagctcgtcg agcgggtcga gcccctcgag cctcgcggcg acgaggcccg
      121 aggccatgcc gaccacggag cccttgcgcg agccgggcac cacgacgcag cgcggcgcca
      181 ccgcggcgac cgcctccggc tcgagctcgg cgtagcaacc ggtgactatg gcgacggcct
      241 cggggttgcg ccgcagggcc agccgcatct cgcggcgggc cttctgctcg gccttgctcg
      301 tcacggtgca ggtattgaag acgacgaggt cggccagctc gccgggaccg acgacaacgg
      361 cgccggcggc cgagaagccg tcggccaggg cctcggtctc ggcctggttg agcttacagc
      421 cgaaggtgcg aaacgagacg cggagggaac ggcccgcggg catgctcgtc agcgcctcgt
      481 cctcgcgagc acgcgctccc ggccgcgtac cgcgtcgacg aggctcggat tgatggcgag
      541 cgcggcatcg taggccgcca gggcgttggc ccagtcggag gcgcgctcgc gcacgtaacc
      601 cagcctagcc caccagcggg cgttgcgagg ctcgtactgc agggccgtgc gcatggcgat
      661 gtcggcgtgc tcgagccgct cgagccgcag gtaggtctcg ccgcttagat aatagacggt
      721 gccgatgcgc gcgccgtccg gcagtaggtt gacgtagtcc ctgaagtggt cgagagcctg
      781 ccggttcttg ccctggtagt aggcggcctc gccgagggac tcgacgatgc gcgggtcgcg
      841 gcgcacggtg gtataggcct tgttcgcgta cagctcggcg tcggcgtagc gttccagcgc
      901 caggagcgac cagacgagca ccacatacga ctcgatgttg agcggatcct gggccaactc
      961 ggccaggcag acggtcctgg cgtcgtcgaa gcggccttcg cggtacatct tgagcgcgtc
     1021 gggcctatcc tgggcgtaga ggccgctcga gaccgacagc gccagggcgg cggctacggc
     1081 gcgggcggcg ttcgggcgcg gtcccatggc tatccgggac gccctcaggc ctgggtcgac
     1141 gaggagtcgc cggtcatcgt gcagataccg ttgaaacggc agcccggttc catcgtcacc
     1201 tcggccgcgg tcacgtcgcc ttcgacgcgg cagctggcgt acagctcgac gcggcccgtg
     1261 gcgacgatgt tgcccttgag ggagccgcgc acggtgacgt tcccggcccg cacgtcggcc
     1321 tcgacgacgg ccttctcgtc gatatggagg tcgctatcgg agacgatcga gccggagacg
     1381 cggcccttga tcatcatcgg ctccttgaag gccatggagc cggcgaactc gacgtccgag
     1441 gcgagtatcg tatcgagggt atcttcatcc accacgctga gatgtacgtc ggtcatagcc
     1501 tgggatgata cggtaaagcc gcgagctagg caagcctgcc gaacgcgtcg tacagctcgg
     1561 agaacagctc cggcgtcagg gtctcggcgc gcgcgtcggg cgatacgccg gctgcctcgc
     1621 aggcggccgc gatcagcggc tcggggcggc cggccgcctt gaggttgttg cgtagcgtct
     1681 tgcgcctgga actgaagcag gcgcgcgtga agccggtgaa ggccttgtcg cccgagtggg
     1741 ccaccgcgtc gggccgggcc tccatcgtga cgacggacga actgacgcga ggctggggcc
     1801 agaacgatcc gggggacagg tcgaagacgg acctgaccgc gtaggccgac tggcaga
//
LOCUS       DAOPHV010000555         1856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_89468_length_1856_cov_3.411993, whole genome shotgun sequence.
ACCESSION   DAOPHV010000555 DAOPHV010000000
VERSION     DAOPHV010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89468_length_1856_cov_3.411993"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLQ29_12015"
     CDS             <1..594
                     /locus_tag="PLQ29_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87412.1"
                     /translation="GLVVVDFVEPNFAAGDMLRTGPHDGPRALVESAVAVPTLPFSMG
                     LELGSQNLGGAFGQYFGSAGGIFGYTCGYEKASALARTIGGMSVSMYYVTPVLGNFLS
                     AAHIGPGLLLGTQFMPEAGFGTAVVPVESIVLVAAGGFVEAEIAGFLVRAGARASLAL
                     GSFDLGYGADFLVDDRYVGLVVEPLLTVGYRLRLTGN"
     gene            603..1232
                     /locus_tag="PLQ29_12020"
     CDS             603..1232
                     /locus_tag="PLQ29_12020"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HPG87413.1"
                     /translation="MKTGTTIRNRAARLAVAALALAAASAACRMDLVKARLAPPELGE
                     FVAYRIGLDELSVGNPVPAYPVGTVLRYTLDGSDPGANAAMLGVGVTIAPVTEQATLR
                     VVASLAGFEDSAVATSVAADLVMTLSTPDLDKLVRGYTTIYLMSLFTGYPDGTVIRYT
                     LDGTEPDETSAGVTKDDDSIYANGPVTMKARAYCAGCRASAVASKFFDD"
     gene            complement(1255..>1856)
                     /locus_tag="PLQ29_12025"
     CDS             complement(1255..>1856)
                     /locus_tag="PLQ29_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPG87414.1"
                     /translation="SPLPDAPMGAYTLLSRDRLLFSATRSSPFPHGEIEFAEDREGPP
                     SRAYRKLWEALVMAGAMPGPGRRCVDAGACPGGWTWALAGLGAEVIAIDRAPIDDRVA
                     AMPGVSFVKHNAFTIKPEDIGPVDWFCSDVICYPPALYDWVSLWLDSGLARNFVCTIK
                     MQGTEWDRQTVARFAAIPGSRVLHLWHNKHELTWLLVRD"
BASE COUNT          281 a          602 c          648 g          325 t
ORIGIN      
        1 gggctggtgg tggtggattt cgtcgaaccg aacttcgccg ccggcgacat gctgcgtacc
       61 ggcccgcacg acggcccccg ggccctggtc gagtcggccg tggcggtgcc gacccttccg
      121 ttctcgatgg gtttggagct cggtagccag aacctgggcg gagcattcgg ccagtatttc
      181 ggctcggccg gtggcatttt cggctatacc tgcggttatg agaaggcgag cgcgctcgcc
      241 cgtaccatcg gcggcatgag cgtctcgatg tactacgtaa cgcccgtgct gggtaatttc
      301 ctatccgccg cccacatcgg gccaggcttg ctcctgggaa cccagttcat gccggaagcc
      361 ggcttcggta ccgcggtcgt acccgtcgaa tcgatagtcc tcgtcgccgc gggcggtttc
      421 gtcgaggccg agattgccgg cttcctggta cgggcgggag cgagggcctc cctggccctc
      481 ggtagcttcg acctcggcta cggagccgat ttcctcgtcg acgaccgcta cgtcggcctc
      541 gtcgtcgagc ccctgctcac cgtcggctac cgcctgagac tgaccgggaa ctaggaggcg
      601 atatgaagac tggaacgacg atacgcaatc gggccgcccg cctcgcggtg gctgcgctgg
      661 cgctggccgc cgcgtcggcc gcctgcagga tggacctcgt caaggccagg ctggcgccgc
      721 ccgagctcgg ggagttcgtc gcgtaccgga taggcttgga cgagctgtcc gtcggcaacc
      781 cggtaccggc ctatccggtc ggcacggtcc tgcgctatac gctcgacggc tccgatcccg
      841 gtgcgaacgc ggccatgctc ggcgtcggcg tgaccatcgc cccggtaacg gaacaggcga
      901 cgctccgggt cgtcgcgagc ctcgccggtt tcgaggattc agccgtcgct actagcgtcg
      961 cggccgactt ggtgatgacc ctgagcacac cggatttgga taagctcgta cgtggctata
     1021 ccacgatcta cctcatgtcg ttgttcacgg gctatccgga tggaaccgtc atccgctaca
     1081 cgctggacgg cacggaaccg gatgagacgt cggccggggt gaccaaggac gacgattcca
     1141 tatacgcgaa cggccctgtt accatgaagg cgagggcgta ctgcgcgggg tgtcgggcct
     1201 ccgcggtagc gtcaaaattc ttcgatgact agtcttcgag ccgtgcggac gcgcctagtc
     1261 cctgaccagg agccaggtca gctcgtgctt gttgtgccag aggtgcagta cgcgggagcc
     1321 ggggatcgcc gcgaagcgcg cgacggtctg gcggtcccac tcggtaccct gcatcttgat
     1381 ggtgcagacg aagttgcgcg ccaggccgga gtcgagccag agcgataccc agtcgtatag
     1441 ggccggcggg tagcagatga cgtcggagca gaaccagtcg actgggccga tgtcctcggg
     1501 tttgatcgtg aacgcgttgt gcttgacgaa gctgacgcct ggcatcgccg cgacccgatc
     1561 gtcgatgggc gcccggtcga tggctatcac ctcggcgccg aggccggcga gggcccaggt
     1621 ccagccgccg gggcaggctc cggcgtcgac gcagcgccgt ccggggccgg gcatggcgcc
     1681 cgccatgacg agggcctccc atagcttgcg gtacgcgcgg ctcggtggtc cctcgcggtc
     1741 ctcggcgaac tcgatctccc cgtgcgggaa cgggctcgag cgcgtggccg agaacagcag
     1801 ccggtcgcgc gagagcagcg tgtaggcgcc catcggcgcg tcgggcagcg gcgacg
//
LOCUS       DAOPHV010000556         1848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_90043_length_1848_cov_2.981037, whole genome shotgun sequence.
ACCESSION   DAOPHV010000556 DAOPHV010000000
VERSION     DAOPHV010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90043_length_1848_cov_2.981037"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PLQ29_12030"
     CDS             complement(<1..594)
                     /locus_tag="PLQ29_12030"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPG87415.1"
                     /translation="MKHLSRRKKAPFLRTILIAAVALPIPAFAASPLPPLVETVVKND
                     AKRTAALIREGADVNVFQEGMDTPLVEAVDREPSKFNLEILKLLLGAKDIQVNKYSAL
                     SMGSYVWERTPLIAAAYNGNAEAVKLLLAKGAKVDLTDTYYPISDVLGPEPRSTALTW
                     AATRGHYDVVVLLVEAGARIEWVNANAITPLIAAASNV"
     gene            complement(919..>1848)
                     /locus_tag="PLQ29_12035"
     CDS             complement(919..>1848)
                     /locus_tag="PLQ29_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPG87416.1"
                     /translation="GPAWSNSLFEDAAEFGMGMRLNADKMGQYARELVAANKAAIGAL
                     ADKLLSNEQADDLSIEAQRAAVSELKKALAGKKDEWAKELVNVADHLIRRSVWIFGGD
                     GWAYDIGFGGLDHVIASGRNVNIMVLDTEVYSNTGGQMSKATPFGAVAKFAAAGKDIG
                     KKDLPMIAMSYGYVYVAHIAMGANMNQALKAIREAESYDGPSIVVAYSHCAMHGIDMM
                     KGMDQQKLVVESGIWPLFRYDPRLVAQGKNPFQLDSKEPQLDKVSTFMGNEIRFRTLR
                     AADAERADMLEAKEKALVERRWREYKYLADRPF"
BASE COUNT          289 a          582 c          630 g          347 t
ORIGIN      
        1 gacgttcgag gcggcggcga tcaggggggt tatcgcattg gcgttgaccc attctatccg
       61 ggcgccggcc tcgacgagga ggacgacgac atcgtagtgg ccgcgcgtgg ccgcccaggt
      121 cagggcggtg gaacgtggct cgggccccag gacgtcgctt atgggatagt aggtgtcggt
      181 caggtcgacc ttggcgccct tggcgaggag cagcttgacg gcctccgcgt tgccgttgta
      241 ggcggccgcg atgagcggcg tccgctccca gacgtagcta cccatggaga gcgcgctgta
      301 cttgttgacc tggatgtcct tggcgcccaa gagcagcttc aggatctcca ggttgaattt
      361 actcggttcc cggtcgacgg cctccacgag cggcgtatcc atgccttcct ggaagacgtt
      421 gacgtcggcc ccctcgcgta tgagggcggc ggtcctcttg gcgtcgttct tgacgaccgt
      481 ctcgacgagg ggcggtagcg gcgacgccgc gaaggccggt atgggcaggg cgacggccgc
      541 gatcagtatc gttcgaagga atggcgcctt cttccgcctg gacagatgct tcaaactcga
      601 cgctcctggg cttggcatga gatctcctga tcgatgggac atatccgaac cactacttga
      661 atgtatgggc atgcgccgag cgatagcaag cgccgacgcc taccgtacga gcccgaaacc
      721 cgacgttcca aggcatgcgg tctcgaggaa ccgttacggg ccgaaaacaa aagcccgccg
      781 gcgcgcgagc gccgacgggc ttccgtaata cagctcagtc caaagcttaa cccgcgcgcg
      841 aagcgcgtcg ggttaaagcg ggcgggagcg gtatcgcggc tccgctagga gtccgcgata
      901 ccctcgaccg accgttcgtc agaacgggcg gtcggccaag tatttgtact ctctccagcg
      961 cctctccacg agcgccttct ccttggcctc gagcatgtcg gcgcgctcgg cgtcggccgc
     1021 ccgcagggtg cggaagcgga tctcgttgcc catgaaggtg gacaccttgt cgagctgcgg
     1081 ctccttggag tcaagctgga aggggttctt gccctgggcg acgaggcgcg ggtcgtagcg
     1141 gaacagcggc cagatgcccg actcgacgac gagcttctgc tggtccatgc ccttcatcat
     1201 gtcgatgccg tgcatggcgc agtggctgta ggcgacgacg atggacgggc cgtcgtagct
     1261 ttccgcctcg cggatggcct tgagcgcctg gttcatgttg gcgcccatcg cgatgtgggc
     1321 cacgtacacg tagccgtagc tcatcgcgat catcggcagg tccttcttgc cgatgtcctt
     1381 gccggccgcg gcgaacttgg ccaccgcgcc gaagggcgtg gccttggaca tctgcccgcc
     1441 ggtgttcgag tacacctcgg tgtcgagcac catgatgttc acgttgcggc cggaggcgat
     1501 cacgtggtcc aggccgccga agccgatgtc gtaggcccag ccgtcgccgc cgaagatcca
     1561 gaccgagcgg cggatcaggt ggtccgccac gttgacgagc tccttggccc actcgtcctt
     1621 cttgccggcg agcgccttct tgagctcgga caccgcggcg cgctgcgcct cgatggacag
     1681 gtcgtcggcc tgctcgttgg acaggagctt gtcggccagg gcgccgatgg cggccttgtt
     1741 ggcggcgacg agctcgcggg cgtactggcc catcttgtcg gcgttgagcc tcatgcccat
     1801 gccgaactcg gcggcgtcct cgaagaggct gttcgaccac gcggggcc
//
LOCUS       DAOPHV010000557         1846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_90183_length_1846_cov_4.009492, whole genome shotgun sequence.
ACCESSION   DAOPHV010000557 DAOPHV010000000
VERSION     DAOPHV010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90183_length_1846_cov_4.009492"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLQ29_12040"
     CDS             complement(<1..237)
                     /locus_tag="PLQ29_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87417.1"
                     /translation="MSTTNRRYARTIVAALAATACALVSACPTPYQGAIDIESRVIRV
                     DLPDVIFNPFSASAPATVRGVGAISAVVTVTTDAD"
     gene            complement(234..>1846)
                     /locus_tag="PLQ29_12045"
     CDS             complement(234..>1846)
                     /locus_tag="PLQ29_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPG87418.1"
                     /translation="YDFYNHVSPAFPITLAAGETSDLLTVGFYPTDNGDLDGRVMFEV
                     ADRVVSVGLTGRGVWQLTLSIHPDTLDEGFDAGQITSPVTVTPASGAVTFLSADGVVK
                     LTALAYIMSKFNAWEVLGTPKVEPDFADDNLKAEDTIVTLNAHTAIQASFYNPYVYFP
                     VAGATPVGRTAYDSLQAAIDACVPSPTLEAVIIKRGSHTVGAGVTLPEGVSIFGGYSA
                     DYATRAYKTPAERSTTNPAILTFAAGTGLSVSAASGQQSVIEGVTLTRSAASATPVIT
                     VGSGAKPVIRYTTITTQGEGPALQSSYGSPRIGDCVITGGAGAGKSVGAYFYGGAPYL
                     EWNDIRSGNTSGNYSEAYAVCFEDNSYPDLYMNTITAGTASGTKSASYGVYLDFGCRP
                     LIVGNTIDGGRAPGSGGISTGVFYINKDGKATVRYNTISGGFGALQARALYAAQGANY
                     YLCYNNLTADQASGSTVGLYLDTNGRVRRLIGNGFVGLGTLQYTWLSDEAIGDIDELN
                     GGYYLSDTDANKAEAISLPGVPSLGVRE"
BASE COUNT          303 a          566 c          639 g          338 t
ORIGIN      
        1 gtcagcgtcg gtcgtgaccg tgacgacggc cgagatggct ccgacgcctc ggaccgtcgc
       61 cggcgcgctc gcggagaacg ggttgaagat cacgtccggc agatcgaccc ggatgacgcg
      121 cgactcgatg tctatcgcgc cctggtaggg cgtcgggcag gcggatacta gcgcgcaggc
      181 ggttgccgcc agggcggcga ctatcgtacg ggcatatcgt cgattcgtcg tactcattcc
      241 ctcaccccca acgaaggcac gccgggcaag cttatcgcct cggccttgtt cgcgtcggtg
      301 tcgctcaggt agtagccgcc gttcagctcg tcgatgtcgc cgatagcctc gtcgctcagc
      361 caggtgtact gtagggtgcc gagcccgacg aagccgttgc cgatgaggcg gcgcacccgt
      421 ccgttcgtgt ccaggtagag gccgacggtc gaacccgagg cctggtcggc cgtcaggttg
      481 ttgtagcaca ggtagtagtt ggcgccctgg gcggcgtaca gggcgcgggc ctggagcgcg
      541 ccgaagccgc cgctgatcgt attgtagcgg accgtcgcct tgccgtcctt gttgatgtag
      601 aagacgccgg tgctgatgcc gccggagccc ggagcgcgtc cgccgtctat cgtgttgccg
      661 acgatcaggg gcctgcagcc gaagtccagg tacacgccgt acgacgccga cttggtgccg
      721 ctcgcggtgc cggccgtgat cgtgttcata taaaggtcgg gataggaatt gtcctcgaag
      781 cacacggcgt aggcctcgga gtaattaccc gaggtgttgc ccgagcgtat gtcgttccac
      841 tccaggtacg gcgcgccgcc gtagaagtag gcgccgaccg acttgccggc acccgcgcct
      901 ccggtgatca cgcagtcgcc gatgcgcggg ctgccgtagc tcgactgcag cgccggacct
      961 tcgccctgcg tcgtgatcgt cgtgtagcgg atgaccggct tagccccgga gccgaccgtg
     1021 atcaccggcg tggcgctcgc ggccgagcgt gtcagcgtga cgccctcgat gacgctctgc
     1081 tggccggagg cggccgatac ggagaggcca gtgccggccg cgaaggtaag gatagcgggg
     1141 ttggtcgtgg accgttcggc gggcgtcttg tacgcccgtg tcgcataatc ggcgctgtag
     1201 ccgccgaaga tcgagacgcc ctcgggcagg gtgacgccgg cgccgaccgt gtgcgacccg
     1261 cgctttatta tgacggcttc gagggtcggg cttgggacac aggcatcgat cgcggcttgc
     1321 agactgtcgt acgcggttct gcctacaggc gtggctccgg cgacggggaa gtagacgtac
     1381 ggattataga agctggcctg gatagccgta tgggcgttga gcgtgacgat cgtgtcctcg
     1441 gccttcaggt tatcatcggc gaagtccggt tctactttcg gcgtgcccag aacttcccag
     1501 gcattgaact tcgacatgat ataggccaag gccgtcagct tgacgactcc gtcggccgat
     1561 aaaaacgtga ccgcacctga agccggagta accgtgaccg gcgacgtgat ctgaccggcg
     1621 tcgaagcctt catcgagcgt atccgggtgt atcgacaacg tcagctgcca cacccctcgg
     1681 ccggtcaggc ccacggagac gacgcggtcg gccacctcga acatcacgcg gccgtccagg
     1741 tcgccgttgt cggtcggata gaagcctacg gtcaggaggt cgctcgtctc gccggccgcg
     1801 agggttatgg ggaaggccgg agagacgtgg ttatagaaat cgtagt
//
LOCUS       DAOPHV010000558         1845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_90272_length_1845_cov_3.753631, whole genome shotgun sequence.
ACCESSION   DAOPHV010000558 DAOPHV010000000
VERSION     DAOPHV010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90272_length_1845_cov_3.753631"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PLQ29_12050"
     CDS             <1..937
                     /locus_tag="PLQ29_12050"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HPG87419.1"
                     /translation="PEPSAGGLLVVCSSTLGFSVPYTVMRALRRAPRAAAGSRAAVAC
                     VCGSTMGRSGIIGGWSGAASIAALAALGRRGYRAAGSVDVSYPENWTQASPPAAGADR
                     DAMIARGDAEAEAFGESLVAGDGTFLRRDPLTRSLGRFVGFVFRVLARRALARLFVAD
                     GACTGCGLCADACPSAAIEMRDGRPAWSTACSACNRCINLCPAAAIQTSTARLVLVLG
                     LNAAAMLAAPRAADALLAAVGGGSGWPGFGLASFALSLALYAALTVLQLGPLDALLRA
                     AERSRALRRIFVAGPTRRFPRYLAPGFSPRIPSDR"
     gene            1073..>1845
                     /locus_tag="PLQ29_12055"
     CDS             1073..>1845
                     /locus_tag="PLQ29_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HPG87420.1"
                     /translation="MSEYLTNDATRAERAARLKVIIDKLAAGATPSAVKKEFHDLIKG
                     ADAAEVADLEQSLIEGGLPVEEVQRLCDVHADVFKAGLERGERAERMPGHPVHTYLAE
                     NKEARKRARSLRLAALGGSLEAVANAVRGLKPIITHFERKENQLFPYLERKGFTGPSK
                     VMWGKHDEIRAVFRELDAATGSAGSKAYRAKARDLARRIRAMIFMEEKILLPNALKRL
                     SDAEWAEVRRGEDAIGFSWVKPGAEWDPALVAPAGGGAYA"
BASE COUNT          262 a          636 c          672 g          275 t
ORIGIN      
        1 gccggagccg tcggccggag ggctcctggt cgtctgcagc tcgacgctcg gcttctccgt
       61 gccgtatacg gtgatgcgcg ccctgaggcg agcgccgcgc gccgcggccg ggtcgcgggc
      121 ggccgtggcc tgcgtctgcg ggtcgacgat gggcaggtcc ggcataatcg gcggctggag
      181 cggggccgcg tcgatcgcgg cgctggcggc gctcgggcgg cggggctacc gggccgccgg
      241 ctcggtcgac gtctcgtacc cggagaactg gacccaggca tcgccgccgg cggcgggcgc
      301 cgaccgggac gcgatgatcg cgcgggggga cgcggaggcg gaggccttcg gcgagagctt
      361 ggtggcgggc gacgggacgt tcctgaggcg cgatccgctg acgaggagcc tcggccgctt
      421 cgtcggcttc gtgttcaggg tcctggcgcg cagggccctg gcgcggctgt tcgtcgccga
      481 cggcgcgtgc accggttgcg gcctctgcgc cgacgcgtgc ccgtccgccg cgatcgagat
      541 gcgcgacggt cggccggcct ggtcgacggc gtgctcggcg tgcaaccgct gcataaacct
      601 ctgcccggcc gccgcgatcc agacatcgac ggctaggctc gtcctggtgc tcgggctcaa
      661 cgccgcggcg atgctagccg cgccgcgcgc cgcggacgca ctgctcgccg ccgtcggcgg
      721 cggttccggc tggcccggct tcggcctcgc gtccttcgcg ctctccctgg cgctctacgc
      781 ggcgctgacc gtcctgcagc tcggcccgct cgacgctctg ctcagggcgg ccgagcgatc
      841 gcgcgcgctt cgccggatct tcgtcgccgg cccgacgagg cgcttcccgc gctacctggc
      901 gccggggttc tcgcctagga ttccgtcaga ccgatagtcc ccggccgcgc ccgccccgat
      961 ttgaccccgt ctcccccatc gacgatcatg taaggcacgc gaaacgagcg cgccatttgt
     1021 ttttttaact tttatgacta ttttgataac ctataagcaa ggaggacgtt caatgagcga
     1081 gtacctgacc aacgacgcga cgagggccga acgggcggcc cggctcaagg tcatcatcga
     1141 caagctggcc gccggagcta ccccgtcggc cgtcaaaaag gaattccacg acctaatcaa
     1201 gggagcggac gccgcggagg tcgccgacct tgagcagtcc ctgatcgagg gaggcctgcc
     1261 ggtcgaggag gtacagcggc tctgcgacgt ccacgcggac gtgttcaagg cgggcctcga
     1321 gcgcggggag cgcgccgagc gcatgcccgg gcatccggtc catacgtacc tggcggagaa
     1381 caaggaagcc aggaagcgcg cgcgctcgct gaggctcgcg gccctcggcg gcagcctcga
     1441 ggcggtagcc aacgccgtgc gcgggctcaa gcctataatc acccatttcg agcgcaagga
     1501 gaaccagctc ttcccctacc tcgagcgcaa gggcttcacc ggcccgtcca aggtgatgtg
     1561 gggtaagcac gacgagatcc gggccgtctt ccgggagctg gacgcggcca cgggctcggc
     1621 gggctccaag gcgtatcgcg ccaaggcccg cgacctggcc aggcggatac gcgcgatgat
     1681 cttcatggaa gagaagatcc tgctaccgaa cgcgctcaag cgcctgagcg acgccgagtg
     1741 ggcggaggtg aggcgcggcg aggacgcgat cggcttctcg tgggtcaagc ccggcgccga
     1801 atgggatccg gcgctcgtcg ctccggccgg cggaggcgcc tacgc
//
LOCUS       DAOPHV010000559         1841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_90536_length_1841_cov_4.823068, whole genome shotgun sequence.
ACCESSION   DAOPHV010000559 DAOPHV010000000
VERSION     DAOPHV010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90536_length_1841_cov_4.823068"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1095)
                     /locus_tag="PLQ29_12060"
     CDS             complement(7..1095)
                     /locus_tag="PLQ29_12060"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPG87421.1"
                     /translation="MPGDDTRKRLLAPFTRSKGARLKRVIVLPFFVVTALGFALSWVL
                     YVNGSRAAVSDAIRAIAVESSKRVSDQAYARLGHAVHVASANAAFLGAFPRIDDETTA
                     IRLAFVEQLRHQPMMAIAAIGMENGDYLEAQRIGPDSYRVGAAGAVTGGSLVFRPVLD
                     DGGFGELYVEAPGYDPRERPWYRAAIEADGLAWSRPYSIFSTNEPAVAVSLPVRRDGE
                     VFGVAVATVELGTLSAYLATVEEARNGVAFVEDEEGRLIASSRSTILDVGGERAVAVI
                     HWDPLVAAAARADGEARSGGDSRAEDSDDPVPFSFTLRGASYLGVSRPVRLGADQAWR
                     AVLAVPQSAYTEKLRQTDAKALYRFKKE"
     gene            1187..1390
                     /gene="thiS"
                     /locus_tag="PLQ29_12065"
     CDS             1187..1390
                     /gene="thiS"
                     /locus_tag="PLQ29_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HPG87422.1"
                     /translation="MTITLNGSTERFDEPSMTVRGLLLAKRWSFPLIVVSINGAAVAR
                     DDWDAATVYDGDVVDATHLMSGG"
     gene            1402..1767
                     /locus_tag="PLQ29_12070"
     CDS             1402..1767
                     /locus_tag="PLQ29_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87423.1"
                     /translation="MTIPGVTKLMHDQCERAMLEGEFGPDVIGSAESVAVVLTQGWCP
                     QWRAMARWLGEAAAEAGARAYYVEYDEEPFFERFMAWKEEALGNRSVPYLRYYRGGEL
                     VAESNYVSRDGFAARLRRG"
BASE COUNT          294 a          575 c          681 g          291 t
ORIGIN      
        1 ggcgctctac tccttcttga agcggtagag cgccttggcg tcggtctggc gcagcttctc
       61 ggtataggcg ctctggggca cggccagtac cgcgcgccag gcctggtcgg cgccgagccg
      121 gacgggcctg gagacgccga ggtagctggc gccccggagc gtgaaggaga acgggacagg
      181 atcgtcgctg tcctcggcgc gggaatcgcc gccggaacgt gcctcgccgt cggcgcgggc
      241 ggcggcggcc acgagcgggt cccaatgtat gacggcgacg gcgcgctctc ccccgacgtc
      301 gagtatcgtc gacctggacg aggctatcag cctgccttcc tcgtcctcga cgaaggcgac
      361 gccgttcctg gcctcctcga cggtggccag gtaggccgag agcgtgccga gctcgacggt
      421 cgcgacggct acgccgaaga cctcgccgtc gcggcgtacg ggtagcgaca cggctacggc
      481 cggctcgttc gtcgagaaga tcgagtaggg acggctccag gcgagtccgt cggcctcgat
      541 cgccgcgcgg taccaggggc gctcgcgggg atcgtagccc ggcgcctcga cgtagagctc
      601 gccgaagccg ccgtcgtcga ggacgggcct gaagacgagc gatccgccgg tgactgcgcc
      661 ggcggcgccg acgcggtacg agtcgggccc gatgcgctgg gcctcgagat agtcgccgtt
      721 ctccatgcct atggccgcta tggccatcat gggctgatgg cgtagctgct cgacgaaggc
      781 gagccgaatg gcggtcgtct cgtcgtcgat ccgggggaat gcgccgagga aggcggcgtt
      841 ggccgacgcg acgtggacgg cgtggccgag cctggcgtag gcctggtccg agacccgctt
      901 gctcgactcg acggcgatag cccggatggc gtcgctcacg gcggcgcggg agccgttgac
      961 gtacaggacc cacgacagcg cgaagccgag cgccgtgacg acgaagaagg gtaggacgat
     1021 gacgcgcttg agcctggcgc ctttcgagcg cgtgaagggc gcgagtagcc ttttgcgtgt
     1081 atcgtctcct ggcatggtgc gtcagtatac tccggcgtta ccgattcagt aaagaaaaat
     1141 acctgaatgg agaaacgcag ccgagacgtg gtgtatattc gatggtatga cgataaccct
     1201 gaacggtagt accgaacggt tcgacgagcc ctcgatgacg gtgcgtggct tgctcctggc
     1261 caagcgctgg tcattcccgc tgatcgtcgt ctcgatcaac ggcgcggccg tggcgaggga
     1321 cgactgggac gcggcgacgg tctacgacgg cgacgtcgtc gacgcgaccc acctgatgtc
     1381 cggcggctga ggagtgaagg cgtgacgatc cccggtgtta cgaagcttat gcacgaccaa
     1441 tgcgaacggg cgatgctcga aggcgagttc ggccccgatg tcatcggctc ggcggaatcg
     1501 gtcgccgtcg tcctgaccca gggctggtgc ccgcagtgga gggcgatggc ccgctggctc
     1561 ggcgaggcgg cggccgaggc cggggcgcgc gcctattacg tcgagtacga cgaggagccg
     1621 ttcttcgagc gcttcatggc ctggaaggag gaggcgctcg gcaaccgcag cgtgccatac
     1681 ctgcgttact accgcggcgg ggagctcgtc gcggagagca actacgtgtc gcgggacggc
     1741 ttcgcggcca ggctgcgccg cggctaaggc agaccgggcg gcccttcgtc gacccgccta
     1801 gtcgcccagg gccttctccg tcagctcgat gagcaggggc a
//
LOCUS       DAOPHV010000560         1834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_91047_length_1834_cov_5.172569, whole genome shotgun sequence.
ACCESSION   DAOPHV010000560 DAOPHV010000000
VERSION     DAOPHV010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91047_length_1834_cov_5.172569"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..1010)
                     /locus_tag="PLQ29_12075"
     CDS             complement(222..1010)
                     /locus_tag="PLQ29_12075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPG87424.1"
                     /translation="MPSKASESEPVRFDEPGWFGDDGFWTDYAPLLFGGERWAEAPAV
                     VDAVLALSGAEPGSPVLDMCCGPGRHALEFASRGHPVVGVDITEPYLEAARDSAEAMG
                     LTAEFVRGDARSWCRPGAFSLAVNLYTSFGYFDSKAEDEAMLARLAASLAPGGALVME
                     MVGKETAARDFNDGEWFERDGRLVLTEFTVVGAWEGLRNRWVFVDADGRRVDRSWVQR
                     LYSATELRDSLLRSGFSSAEAYGSWAGAPYDQSAERLIAVARKA"
     gene            complement(1000..1377)
                     /locus_tag="PLQ29_12080"
     CDS             complement(1000..1377)
                     /locus_tag="PLQ29_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87425.1"
                     /translation="MQAIDRLKSDLDDWVRRSGRAPGNREFDSLRDTFARSVPKVPGE
                     TMAALFTTFLSRYGSGGSEYSAKAIDWLAGVGSLLLSDYDGTPFTREDWEEIRDIVTV
                     ESGEIDMDTLSYVLGQALEHGAV"
     gene            complement(1594..>1834)
                     /locus_tag="PLQ29_12085"
     CDS             complement(1594..>1834)
                     /locus_tag="PLQ29_12085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HPG87426.1"
                     /translation="LGALTGRGAGAVAAAAVSTGLFVAGHAYQGPAGMLASAIAGSAL
                     AAARLRGARLHALAWGHAAYDLAVLIAGFGLAARA"
BASE COUNT          312 a          605 c          596 g          321 t
ORIGIN      
        1 cgtgacgacg agtcgcttct tggccgccgc gccgtcttcg agaatcgaat agaatttgcc
       61 gtccgctagg cggattccga tcttagccat ggcttcttcc gttctgacca gattgtaatg
      121 ggatacgact acccttagta gtatcggccc atttcggcaa aaaatcgaga ggcaaccgtc
      181 ttggaatccc gttggatcgc gaaaggcgac gcctggccgt gttatgcctt cctggcgacg
      241 gctatcaaac gctcggcgga ctggtcgtag ggagcaccgg cccaggaacc gtatgcctcg
      301 gccgacgaga aaccggagcg gaggaggctg tcgcgaagct ccgtggccga gtagagccgc
      361 tgcacccagg agcgatcgac gcgcctgccg tcggcgtcca cgaagaccca gcggttcctc
      421 aggccctccc aggctccgac caccgtgaac tcggtcagta cgaggcgtcc gtcgcgctcg
      481 aaccattcgc cgtcgttgaa gtcgcgcgcg gcggtctcct tgccgaccat ttccatgacg
      541 agcgcgccgc cgggcgcgag gcttgcggcg agcctcgcga gcatcgcctc gtcctcggcc
      601 ttcgagtcga agtagccgaa gctggtgtac aggttgacgg cgagcgagaa cgcgcctggc
      661 cggcaccatg atcgggcgtc gccgcgtacg aattcggccg ttagccccat cgcctcggcg
      721 ctgtcccggg ccgcctccag gtacggctcg gtgatgtcga cgccgaccac cgggtggccg
      781 cgcgaggcga attccagcgc atgcctacca ggaccgcagc acatgtcgag taccggcgac
      841 ccgggctcgg ccccgctcag ggccaggacg gcgtcgacga cggccggcgc ctccgcccag
      901 cgctcgcctc cgaacagcag gggcgcgtag tcggtccaga agccgtcgtc tccaaaccat
      961 cctggttcat cgaaccgtac cggttcggat tctgaagcct tagacggcac cgtgctcgag
     1021 cgcctgtccg agcacgtagc tcaaggtgtc catgtctatc tcgccggact ctacggtgac
     1081 gatatcgcgt atttcctccc agtcctcccg ggtgaacggc gtgccgtcgt agtcggacag
     1141 gagtagcgag ccgactccgg ccagccaatc gatggccttg gccgagtatt ccgagccgcc
     1201 cgagccgtag cgggacagga aggtggtgaa gagggcggcc atcgtctcgc cgggcacctt
     1261 gggaaccgat cgcgcgaagg tgtcgcgtag cgagtcgaac tcgcggttgc ccggagctcg
     1321 gccggatcgg cgaacccagt cgtccaggtc ggactttaag cggtctattg cttgcatcac
     1381 cctataatac agtctgccgt gctacttggg tagtgttacg aaccttgttg atgggccgta
     1441 cgcgtgtttg agtgttacga atcttcgtgg tatcgcagtt ccgacagatg acgacttccc
     1501 aataaagccg ttcgccgagg tggaatcgct gctagcgggc cagcggcgcg gctgatacgc
     1561 gctctttgcg tgtcccccat aagattcgta acactatgcg cgggccgcga ggccgaaccc
     1621 ggcgatcaat acggcgagat cgtaggccgc gtggccccat gccagcgcgt ggagtctggc
     1681 gcctcgtagc ctggcggccg ccaaggccga gcccgcgatg gccgaggcca gcatcccggc
     1741 cggcccctgg taggcgtgcc cggctacgaa cagccccgtc gatacggcgg ccgcggcgac
     1801 ggccccggcg ccgcggccgg tcagcgcgcc gagc
//
LOCUS       DAOPHV010000561         1831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_91245_length_1831_cov_4.685248, whole genome shotgun sequence.
ACCESSION   DAOPHV010000561 DAOPHV010000000
VERSION     DAOPHV010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91245_length_1831_cov_4.685248"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PLQ29_12090"
     CDS             complement(<1..790)
                     /locus_tag="PLQ29_12090"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HPG87427.1"
                     /translation="MDRLYVSADIEGVCGIVDWKETEIGEAQGAYFRAEMTSEVAAAC
                     EAAVASGVTDILVKDAHGSGRSIDPSRLPREARVLRAWTRDPYVMMAGLEPSHEGVFF
                     IGYHSAAGTAGSPLAHTMDTNNVAVRINGELASEFLINAYIAAHVGVPVLLLAGDAAL
                     CATAASVNPNIRTVAVSEGRGNASISIHPALAQERIAAAAADALAADPRRMLVELPAS
                     FDVSIHFKQHWLANRGSYYPGARRLGAHEVGFSSKDWGAVMAFLF"
     gene            complement(949..>1831)
                     /locus_tag="PLQ29_12095"
     CDS             complement(949..>1831)
                     /locus_tag="PLQ29_12095"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPG87428.1"
                     /translation="ACGHDAHTAILLGVARWFAERRAELAGNVKLLFQPAEETVGGAA
                     TMIADGCLEHPRVDFVLGLHVMPYLPVGRVETRKGALNGSSTDLRVVVRGLGCHAAYP
                     ERGVDAVMIAAQVVNALHTLVSRSVSPLDSAVLTIGTIQGGTASNVVADEVTMNATLR
                     TASDEVRDLLVSKARAIVEGVPAALGGSGSLEVAYGYAALVNDSDVVDVVTAAADDVL
                     CPGNLEWKERPSMGVEDFSFFCKERPGAFYHLGCGNLGRGIVAPLHASTFDLDEDCLP
                     LGVAMQVSCALRLLERI"
BASE COUNT          300 a          611 c          633 g          287 t
ORIGIN      
        1 agaacaggaa ggccatcacc gcgccccagt ccttggacga gaagcctacc tcgtgggcgc
       61 cgaggcgcct ggcgcccgga tagtacgagc cccggttggc cagccagtgc tgcttgaagt
      121 gtatcgacac gtcgaaggac gcgggtagct cgacgagcat gcgcctcggg tcggccgcga
      181 gcgcgtcggc cgccgcggcc gcgatgcgct cctgggccag agccgggtgt atcgatatcg
      241 acgcgttgcc gcggccctcg gatacggcta cggtgcggat gttcggattg accgaggccg
      301 cggtggcgca gagggccgcg tcgccggcga gcagcaggac gggcacgccg acgtgggcgg
      361 ctatgtacgc gttgatcagg aactcggacg cgagttcgcc gttgatcctg acggccacgt
      421 tgttcgtgtc catcgtatgc gcgagcgggc tgcctgcggt gccggccgcg gaatggtagc
      481 cgatgaagaa tacgccctcg tggctcggct cgaggccggc catcatcacg tagggatcgc
      541 gggtccaggc gcgcaggacg cgggcctcgc gcggcaggcg cgacgggtct atcgagcggc
      601 cggagccatg ggcgtccttg acgaggatat ccgtgacgcc cgaggcgacc gccgcctcgc
      661 aggccgccgc cacctcgctc gtcatctccg ccctgaagta ggcgccctga gcctcgccta
      721 tctcggtttc cttccagtcg acgatgccgc agacgccctc tatgtccgcg ctgacgtaca
      781 gcctatccat ggatcacctc caccttcaac gtaaatacct taccacggag acacggaggt
      841 caagaacatg gtatgtagtg atggaaatcg cggcaaagcg atcctgccat gtacatgacc
      901 ttttcaagtt ctctccgtgc ctccgtgact ccgtggttca ttctccgatc atatccgttc
      961 caggaggcgt agggcgcagg acacctgcat cgcgacgccg aggggcaggc agtcctcgtc
     1021 gaggtcgaag gtcgacgcat ggagcggggc gacgatcccg cggccgaggt tgccgcaacc
     1081 caggtggtag aaggcgcccg gccgctcctt gcagaagaac gagaagtcct cgacgcccat
     1141 gctcggccgt tccttccact cgaggttgcc ggggcacagg acgtcgtcgg ccgcggcagt
     1201 gacgacgtcg accacgtcgg agtcgttgac gagggccgcg tagccgtagg cgacctcgag
     1261 cgagcccgag ccgccgagcg ccgccgggac gccctcgacg atggcacggg ccttgctcac
     1321 gagcaggtcg cggacctcgt ccgaggcggt acgcagcgta gcgttcatcg tcacttcgtc
     1381 ggcgaccacg ttcgacgccg tgccgccctg gatcgtgcct atcgtcagca cggccgaatc
     1441 gaggggagac acggagcgcg agacgagggt atggagcgcg ttcacgacct gggccgcgat
     1501 catcacggcg tcgactccgc gctccggata ggccgcgtgg cagcccagtc cccggacgac
     1561 cacgcggagg tccgtactgg agccgttcag ggcgcccttc ctggtctcga cgcggccgac
     1621 cggcaggtag ggcatcacgt ggaggccgag gacgaagtcg acgcgcggat gctccaggca
     1681 gccgtcggct atcatcgtcg ccgcgccgcc cacggtctcc tcggccggct ggaacaggag
     1741 cttcacgttg ccggccagct cggcccgcct ctcggcgaac cacctggcga cgccgagcag
     1801 gatggccgta tgggcgtcgt ggccgcaggc g
//
LOCUS       DAOPHV010000562         1828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_91528_length_1828_cov_2.146080, whole genome shotgun sequence.
ACCESSION   DAOPHV010000562 DAOPHV010000000
VERSION     DAOPHV010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91528_length_1828_cov_2.146080"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PLQ29_12100"
     CDS             <1..822
                     /locus_tag="PLQ29_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87429.1"
                     /translation="NRTHRTRAFAIAVGAIVFASCQSLTYTAEGSMPPHARPVLLARP
                     VYIADLIPPFEVDVDAFNANLFVGRIVFSGGVIRDATRGNIYRSARVALEGEASYRSS
                     IGDLVYDAVARGLRWPNVRGIDGVVSEGRIVASFTRRSDLVQRDGRFLRVDSINVPYR
                     EYTVGLVPGSLSMLRELGGSSDGIFVVPFIEYGYSHTAGWFNDQESGCLAGVRLAVHI
                     LGIDLATGETVFSFAEDFRIVDDFSSVLSEFAIAELFGSIGDTLTDHIGAAIGKR"
     gene            complement(843..1631)
                     /locus_tag="PLQ29_12105"
     CDS             complement(843..1631)
                     /locus_tag="PLQ29_12105"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HPG87430.1"
                     /translation="MPVPLRSDKYREPSLFSPGRFLEYMDETGFKGTGENAAPPEAVI
                     LSYQPALFAYVTRNHPVRFHPGMFRKKLAYLEETGGRVAIMGGFGFGAPAAVATFEEL
                     MALGARSFVSMGTAGALVPGLRPGDLVLCDGAFRDEGTSYHYMPDGGPALPDEALTEA
                     LAEAIGEAGIGFRRGRSWTTDAPYRETRAEIDAFAAEGALVVEMEASALFTVARYRGV
                     ALASAFTVSDSLAEPEWKPDFLSKPTNAGLETLYRAALTALAAG"
BASE COUNT          316 a          581 c          567 g          364 t
ORIGIN      
        1 aatcgtacgc acaggacccg tgccttcgcg atagcggtcg gcgcgatagt cttcgcttcc
       61 tgccagagcc tgacgtatac cgccgaaggg agtatgcctc cgcatgcccg tcccgtactc
      121 ctggcaaggc ccgtatatat agccgaccta atcccgccct tcgaggtcga cgtcgacgct
      181 ttcaatgcca acctgtttgt cgggcgcata gttttttctg gcggcgtgat ccgggacgcg
      241 acgaggggca atatctaccg ctcggcccgg gtcgctctcg aaggcgaggc atcctaccgc
      301 tcatcgatag gcgacctcgt ctacgatgcc gtcgcaagag gcctgcgctg gcctaacgta
      361 cgcgggatag acggggtagt atcggaaggg cgcatcgtcg cctcgttcac gaggcggtcg
      421 gatctagtcc agcgcgatgg gcgcttcttg cgcgtcgatt cgatcaacgt tccgtatcgg
      481 gagtataccg tcggcctcgt acccggttct ttgagtatgc ttcgcgaact gggcggctcg
      541 agcgacggga tctttgttgt cccgttcatc gagtatggtt attcgcatac ggccggatgg
      601 ttcaacgacc aggaatccgg atgcctggca ggcgtacgct tggcggttca catactcggt
      661 atcgacctgg cgacgggaga gacggtgttc tcgttcgccg aggatttccg tatcgtggac
      721 gatttctcct ctgtgcttag cgaattcgcg atagccgagc tattcggatc gatcggagac
      781 acgcttaccg accatattgg cgcggcgatc ggtaaacgct gataggagcc gtatttcggg
      841 cttcaccccg ctgctaaagc cgtcagggcg gcgcggtaga gcgtctcgag gccggcgttg
      901 gtcggcttgg acaggaagtc gggcttccat tccggctccg cgagcgagtc gctgacggta
      961 aaggccgagg ccagggccac gccgcggtag cgggccaccg tgaacagcgc cgacgcttcc
     1021 atctccacga ccagggctcc ctcggccgcg aaggcgtcga tctcggccct cgtctcgcgg
     1081 tacggagcgt ccgtcgtcca gctcctgcct cggcggaagc ctatgcctgc ttcgcctatg
     1141 gcctcggcca gggcttcggt cagcgcctcg tcgggcaggg ccggaccgcc gtcgggcatg
     1201 taatggtacg acgtcccctc gtcgcggaaa gcgccgtcgc acagcaccag gtctccgggc
     1261 cgcaggccgg gcacgagcgc cccggcggtg cccatggaca cgaacgagcg cgccccgagc
     1321 gccataagct cctcgaacgt ggcgacggcg gcaggcgccc cgaagccgaa gccgcccatg
     1381 atggccaccc tgccgccggt ctcctccagg taggccagct tcttcctgaa catgcccggg
     1441 tggaagcgga ccggatggtt cctcgtcacg taggcgaaca gcgccggttg gtacgacagg
     1501 atcaccgcct cgggaggcgc cgcgttctcg ccggtcccct tgaagccggt ctcgtccatg
     1561 tattccagga agcgccccgg gctgaacagc gacggctcgc ggtacttgtc agaacgcagc
     1621 ggcacgggca tggcatcctc ctcgtctagc gctccagtat acgccagagg gcttgtacga
     1681 cggaatgcgg aacaggatga tcaggatgaa ataaagatga acaagatgaa gacctaagct
     1741 ttagcttttc ctatcgtgtc catcctgtcc acatcttgtc catcttgttc cgcctttttc
     1801 ctatcccctc agcgccttta cgcgaaat
//
LOCUS       DAOPHV010000563         1818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_92246_length_1818_cov_3.742484, whole genome shotgun sequence.
ACCESSION   DAOPHV010000563 DAOPHV010000000
VERSION     DAOPHV010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92246_length_1818_cov_3.742484"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="PLQ29_12110"
     CDS             complement(<1..1063)
                     /locus_tag="PLQ29_12110"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting membrane transporter"
                     /protein_id="HPG87431.1"
                     /translation="MRLDDTKKRSYAAMAARGLGSVKPDIPTISVGLGTCGIGNGADV
                     LFDALRDASDGRVAIKKAGCFGFCAEEPLVMLYRPRLPILLFSDMKASKAGKLVTAAL
                     DDAACAKLAKTARAKISSWDFRTSTIDYGHGFDALPEWNELAFFQGQEKLVLRDCGLI
                     DPESLAEYAAVGGYSALLKAIEGEPEDVIDEVTRAKLRGRGGAGFPTGVKWKLMRQNE
                     APIKYVVCNADEGDPGAYMNRNEIESDPHMLLEGMAIGAYAMGAAEGLVYVRAEYPLA
                     VERLKKAIADATAAGFLGERIMGSGFSFAVRIVTGAGAFVCGEETALIASVEGKAGRP
                     SPRPPFPAQKGYRGMPTNIN"
     gene            complement(1060..1542)
                     /locus_tag="PLQ29_12115"
     CDS             complement(1060..1542)
                     /locus_tag="PLQ29_12115"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPG87432.1"
                     /translation="MSPARDASRQPSPGALLTALEEAQRADEYDHLSEDTLRKVAKDC
                     GEPLSRVYSVATFYSFFNLKPQGKHTIIVCRGTACHTRGSLNLLREAAARLGMPGFRE
                     DEESSFTTDDRLFSVRTVACFGQCALAPVVSIDGTIHSRMTQGRLCELLDAIRKGAKR
                     "
BASE COUNT          301 a          571 c          593 g          353 t
ORIGIN      
        1 tattgatgtt cgtcggcatg ccccggtagc ccttctgggc cgggaagggg ggcctcggcg
       61 acgggcgccc ggccttgcct tcgacgctgg ctatcagcgc cgtctcctcg ccgcagacga
      121 aggcgccggc gccggtgaca atcctgacgg cgaaggagaa gccgctaccc atgatacgct
      181 cgccgaggaa gccggccgcc gtggcgtcgg ctatggcctt cttgaggcgc tcgacggcca
      241 aggggtattc ggcgcgcacg tagacgaggc cctcggccgc gcccatcgcg taggcgccta
      301 tggccatgcc ttcgagcagc atgtgcgggt cgctctctat ctcgttccgg ttcatgtacg
      361 cgccggggtc gccctcgtcc gcgttgcaga cgacgtactt gatcggggcc tcgttctggc
      421 gcatcagctt ccatttcacg ccggtcggga agcctgcgcc cccgcggccg cgcaacttgg
      481 ccctggtcac ctcgtcgatg acgtcttccg gctcgccctc gattgccttg aggagggccg
      541 agtagcctcc gacggccgcg tactccgcca gactctcggg atcgatgagg ccgcagtccc
      601 gcaggaccag tttctcctgg ccctggaaga aggcaagctc gttccattcg ggcagcgcgt
      661 cgaagccgtg gccgtaatcg atcgtggagg tcctgaagtc ccaactcgat atcttggccc
      721 tggccgtctt cgccagcttg gcgcaggccg cgtcgtcgag ggcggcggtc accagcttgc
      781 cggccttgga cgccttcatg tccgagaaga gcagtatggg gagcctcggc cggtagagca
      841 tgaccaacgg ctcctcggcg cagaatccga agcaacccgc cttcttgatg gcgaccctgc
      901 cgtccgaggc gtcgcggagc gcgtcgaaca ggacgtcggc cccgttgccg atgccgcagg
      961 tgccgagccc gacggatatg gtcgggatgt ccggcttgac cgagccgagg ccgcgggcgg
     1021 ccatcgcggc gtaggagcgt ttcttcgtat cgtcgagcct catcgcttgg ctcctttgcg
     1081 tatcgcgtcg agcagttcgc atagcctgcc ctgggtcata cgcgagtgga tcgtgccgtc
     1141 tatcgatacg accggggcca gggcgcattg gccgaagcac gcgacggtcc tgacgctgaa
     1201 gagcctgtcg tcggtcgtga acgacgactc ctcgtcctcc ctgaagccgg gcatgccgag
     1261 cctggccgcc gcctcgcgga gcaggttgag cgacccgcgc gtatggcagg cggtaccgcg
     1321 gcagacgatg atcgtatgct taccctgcgg cttcaggttg aagaacgaat agaatgtggc
     1381 gaccgagtag accctggaca gcggctcccc gcagtccttg gccaccttcc tgagggtatc
     1441 ttcggataga tgatcgtatt cgtccgctct ctgggcttct tcgagagcgg tgaggagggc
     1501 tccggggcta ggctggcggc tagcgtctcg agcgggcgac atcgtcgggg acagagtatc
     1561 catcgatcct cccttacgta tcgccgcatg gtgcgaagca tagtcaggga gcgcgttgcc
     1621 ttacgatcga gcgtggcgat cgatgtaatc atatcccttt ttgccatttg tgcaagaaaa
     1681 taatatagat aataaactat gtacaagcca ggtgtcgttc ttgttgttgc acgaacgctg
     1741 ttgccgcaat acacttgtac cgatggacgc acgcgacgag tcatcgccac ggaaaaagag
     1801 acggagcggc ccgatgcg
//
LOCUS       DAOPHV010000564         1815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_92491_length_1815_cov_3.573864, whole genome shotgun sequence.
ACCESSION   DAOPHV010000564 DAOPHV010000000
VERSION     DAOPHV010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92491_length_1815_cov_3.573864"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLQ29_12120"
     CDS             complement(<1..424)
                     /locus_tag="PLQ29_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87433.1"
                     /translation="MLAPAARVSAQASSGTDVSSSALTIGWAELAVSDGVGDEYRNAA
                     VLVPRQLQRALAFADLRFPSDVEAAAASTRAALAAEELARKAVADARAKRDLAALAVR
                     DPAKRGRDVATAAAAVKKAEAALEAILATNRETNRVRSS"
     gene            complement(483..1620)
                     /gene="prfB"
                     /locus_tag="PLQ29_12125"
     CDS             complement(join(483..1550,1552..1620))
                     /gene="prfB"
                     /locus_tag="PLQ29_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002704758.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPG87434.1"
                     /translation="MLEDYMAPIAQLKKEVLEIWGRLDAATIRSKIETKESQSSEPGF
                     WNDQKRSERIMGELKVLRNRLETWEEIKRSTDALDEIYAMAIEEGEGSLEGEIASSLA
                     GVRDRFDKAVTLELMTGEADRTGVFLSIHAGSGGTEACDWAGMLMRMYTRWAERRGFK
                     YEIVDMLEAEGGIKSVTMEIDGAYAYGHLRGESGVHRLVRISPFDANARRHTSFASVS
                     AFPVIDDSIDVTVKPDEIRIDTYRAGGAGGQKVNKTDSAVRITHLATGIVVTCQNERS
                     QHKNKDVAMQVLKSRLYDLYRKEKDKENEKYVGDKKEIAWGSQIRSYVFQPYTMVKDH
                     RTKFFVGNIHGVMDGDLDPFIDSYLHFLWKGGTVQADGDDDDEV"
     gene            complement(1613..>1815)
                     /locus_tag="PLQ29_12130"
     CDS             complement(1613..>1815)
                     /locus_tag="PLQ29_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87435.1"
                     /translation="RATEKQLVALKRDIDKVTDYYDTVRIYQYPVEGRLAISILKENR
                     WKRVVVRAPAEKAPERPRRDHA"
BASE COUNT          266 a          596 c          599 g          354 t
ORIGIN      
        1 ccgaggagcg cacgcggttc gtctcgcggt tcgttgcaag gatggcctcg agggcggcct
       61 cggccttctt gactgcggcg gccgccgttg ccacatcgcg accgcgcttg gcgggatcgc
      121 ggacggccag ggcggcgaga tcgcgcttgg ccctcgcgtc ggccacggcc ttcctggcca
      181 gttcctcggc ggccagggcg gcccgcgtcg aggcagccgc ggcctcgacg tcgctcggga
      241 agcgcaggtc agcgaaggcc agggcgcgct ggagctgacg gggcacgagc accgcggcgt
      301 tgcggtactc gtcgccgaca ccgtcggaga cggcgagctc ggcccagcct atcgtcaacg
      361 cggaggacga tacgtcagta cccgacgaag cctgggccga cacccgcgcc gccggcgcga
      421 gcatcgccga gaacgctacg atacaggcta acccgcgggc cgacggcctt ctcatcggag
      481 ccctacacct cgtcgtcgtc gtcgccgtca gcctggacgg tgccgccctt ccataggaag
      541 tggaggtacg agtcgatgaa cgggtcgagg tcgccgtcca tgacgccgtg gatgttgccg
      601 acgaaaaact tggtccggtg atccttgacc atcgtatagg gctggaacac gtaggagcgt
      661 atctgggagc cccaggctat ctccttcttg tcgccgacgt acttctcgtt ctccttgtct
      721 ttctccttgc ggtacaggtc gtagagccgg ctcttgagca cctgcatcgc cacgtccttg
      781 ttcttgtgct ggctgcgctc gttctggcag gtgacgacga tgccggtggc gaggtgggtg
      841 atgcgcactg cggaatcggt tttgttgacc ttctggccgc cggcgccgcc ggcccggtag
      901 gtatcgatac gtatctcgtc gggcttgacc gtcacatcga tactgtcgtc gatcaccgga
      961 aaggccgaga cgctcgcgaa gctcgtgtgc ctgcgcgcgt tcgcgtcgaa ggggctgatg
     1021 cgcacgagac ggtgcacgcc ggactcgccg cgcaggtggc cgtaggcgta ggcgccgtcg
     1081 atctccatcg tcacgctctt gatgccgccc tcggcctcga gcatgtcgac gatctcgtac
     1141 ttgaagccac ggcgctccgc ccatcgggtg tacatgcgca tcagcatgcc cgcccagtcg
     1201 caggcctcgg tgccgcccga gcccgcgtgg atggacagga agacgccggt gcggtcggcc
     1261 tcgccggtca tcagctcgag cgtcaccgcc ttgtcgaagc gatcacggac gccggcgagc
     1321 gacgaggcta tttccccctc gagggagccc tcgccctcct ctatcgccat ggcgtagatc
     1381 tcgtcgaggg catcggtcga gcgcttgatc tcctcccagg tctcgagccg gttgcgcagc
     1441 accttgagct cgcccatgat gcgctcggag cgcttctggt cgttccagaa gccgggctcg
     1501 gacgactggg actccttcgt ctctatcttg gagcgtatgg tagcggcgtc aaagacgccc
     1561 ccagatttca aggacttctt tcttgagctg cgctatcggc gccatgtagt cttcaagcat
     1621 ggtctctcct cgggcgttcg ggggccttct cggccggcgc cctgacgacg acccgcttcc
     1681 atcgattctc cttgaggatc gagatggcga gccgtccctc gacggggtat tggtagatgc
     1741 ggacggtgtc gtaatagtcc gtcaccttgt cgatatcgcg cttgagcgcg acgagctgct
     1801 tctcggtggc gcgca
//
LOCUS       DAOPHV010000565         1808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_92998_length_1808_cov_4.124929, whole genome shotgun sequence.
ACCESSION   DAOPHV010000565 DAOPHV010000000
VERSION     DAOPHV010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92998_length_1808_cov_4.124929"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PLQ29_12135"
     CDS             complement(<1..1004)
                     /locus_tag="PLQ29_12135"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HPG87436.1"
                     /translation="MVKASGFIISFIALAAAYSCASAPSSGSLDGDTGTAGGMAGGAL
                     VAGLLLDSKEARRVPARKPRAMLAIASPRVLGKAIGMGVLEEKGYPTWRLSRSEALSD
                     VVKVRIGFSARLPEGGVERQSGMLSIPAPSGGGRRELSWIVFLKGSEHHRDKVPSRRG
                     ANESALMDAAAALGYAVWAPDYAGMGDAEGAQEYCVPESMAASALDGLAAARAYLADR
                     GDEYAESGRLYIIGYSQGGIATMASIKAATGGDIPVPGLRIVAAYPLGAALGIVLGGV
                     IAEKYGWRHAFGIVAIPGLVVALLFLRVRDYRTVDLVAAGSAAGGRAERRMKAPDIVR
                     T"
     gene            1101..>1808
                     /locus_tag="PLQ29_12140"
     CDS             1101..>1808
                     /locus_tag="PLQ29_12140"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG87437.1"
                     /translation="MLVIPRRCTDRVCTADPDSRRLERARSSLRRSFGAWIEASADRG
                     SIRLEGEAPSYDDKVRAGWLAARYGFKGVVNDARSPEEAAERLTPRGTAAPSLDGSYY
                     DVAIVGGGVIGCAIARELSRWDLSIVLLEKESDVAAHTSSRNDGMIHDGFAAKPGSKK
                     AAYNVRGNRLWEPLCIELGLDFKRPGSLVLYGNAAAAALYPAMAARARRNGVDGWEFW
                     SRARVAAEEPNVTPDQHG"
BASE COUNT          318 a          589 c          613 g          288 t
ORIGIN      
        1 gtccgcacga tgtccggggc cttcatccgg cgttcagcgc gccctccagc cgccgatccg
       61 gcggcaacca gatcgacggt tcggtagtcg cgaaccctta agaacagaag cgccacgacg
      121 agtccgggga tcgcgacgat gccgaacgcg tgacgccagc cgtatttttc agcgatgaca
      181 ccgccgagta ctatgccgag ggccgcgccg agcggatacg cggcgacgat gcgcaggccg
      241 gggacgggga tgtcgcctcc cgtcgccgcc ttgatcgagg ccatcgtcgc tatgccgccc
      301 tgcgagtagc cgatgatata cagcctgccc gactcggcgt actcgtcgcc tctgtcggcc
      361 aggtaggccc tggccgcggc cagcccgtcg agggcggagg cggccatcga ctcgggcacg
      421 cagtattcct gggctccctc ggcgtcgccc atacccgcgt agtcgggagc ccataccgcg
      481 tagccgaggg cggcggcggc gtccatgagg gcggactcgt tggcgcccct acgtgacgga
      541 accttgtcgc gatggtgctc ggagcctttc aggaagacga tccagctcag ctcgcgcctt
      601 ccgccgccgg acggagccgg gatcgacagc atgccgctct ggcgctcgac tcctccctcc
      661 ggaagcctgg ccgagaagcc tattctgacc ttgaccacgt cggacagagc ctcgctacgc
      721 gacagcctcc aggtcggata gcccttctcc tcgaggacgc ccatgccgat ggccttaccg
      781 agcactcgcg gcgaggctat ggccagcatg gcgcgcggct ttctggccgg cacgcgcctg
      841 gcttccttcg agtcgagcaa gaggcccgcg acgagggcgc cgccggccat gccgccagcg
      901 gttccggtgt cgccgtccaa ggaccccgat gatggagcgc tcgcgcagga gtaggcggcg
      961 gctagggcga tgaacgagat gatgaagccg gatgctttta ccattggatg aacatatagc
     1021 gcgacgacga cgatcgtcca tagcgctgca ccgggtaaaa ataacttgac gatacggtat
     1081 tcatggatta taaaactggt atgctggtaa taccaagaag gtgtaccgat agagtttgta
     1141 ccgcagaccc cgactcgagg cggctggagc gagcgcgctc gtcgcttcgg aggagtttcg
     1201 gtgcctggat cgaggcgtca gccgatcgcg gctcgatacg gctcgagggt gaggcgccga
     1261 gctacgatga taaggtacga gccggttggc tggcggcccg ctacgggttc aagggcgtcg
     1321 tgaacgatgc ccgctccccg gaagaagccg ccgagcggct gacaccgcgc ggcaccgccg
     1381 ctccatcgct cgacggctcg tattacgacg tggccatcgt cggcggcggc gtgatcggct
     1441 gcgcgatagc cagggaactg tcgcgctggg atctatccat cgtcctcctc gagaaggaga
     1501 gcgacgtggc ggcccatacc tcgagccgta acgacggcat gatccacgac ggcttcgcgg
     1561 cgaagcccgg atcgaagaag gccgcgtaca acgtccgggg caaccggctc tgggagcccc
     1621 tgtgcatcga actcggcctg gacttcaagc ggccaggatc gctcgtattg tacgggaacg
     1681 cggctgccgc cgccctgtat ccagccatgg ccgcccgggc gcgccgcaac ggggtggacg
     1741 gctgggagtt ctggagccgc gccagggtgg cggccgagga accgaacgtg acgcctgacc
     1801 agcatggc
//
LOCUS       DAOPHV010000566         1807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_93088_length_1807_cov_4.244292, whole genome shotgun sequence.
ACCESSION   DAOPHV010000566 DAOPHV010000000
VERSION     DAOPHV010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93088_length_1807_cov_4.244292"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PLQ29_12145"
     CDS             <1..754
                     /locus_tag="PLQ29_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87438.1"
                     /translation="RVSPGAGVESERGEYAFSVSLRVDSSLVIVPTGSFGAMFGSARA
                     PDALPPAVSSFRAEASAYVAGSFGSASLTGGTAVSVSAAMAPNLDVGAGMEINEFESV
                     AGRLGARLSTRLGDGLAAAVLVDGRIAEGTVGEPAWARLGAALGVGTPFLNAVAMPAF
                     AAYWEDGCSLRAGLGAAFNLSGYAFGASLSASALTGPLSGGLELDWPIGVTLELRFLP
                     PGLPLAFRLSGGVDWSPAPTGWNLGLAVSGGF"
     gene            complement(761..>1807)
                     /locus_tag="PLQ29_12150"
     CDS             complement(761..>1807)
                     /locus_tag="PLQ29_12150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG87439.1"
                     /translation="RRGLTAEVGEIKGRLKAKLYEFVKEFNIDVLIVENAFAIPMHIP
                     LGIALAELVAELEMPTVAHNHDFAWERQRFMTGCAEDYIVRAFPPRLHSVYHVVINSE
                     ARRQLAFRCGISSVMIPNVWDFDAEPTEADEYNAGLRSELGMGDEEVLFLQPTRIVAR
                     KGIESAIELVARYNGTVGPNQRGRLLISHPDLDEGDEYQHRVLDYAKYMDVELIVRPD
                     LIDVNRGTRQDGGKVYSLWDTYVRADFVTYPSTYEGFGNAFLEAIFYRKPILVNRYAI
                     YAQDIEPLGFRTTVMDGYVTDAVVKDVARMLSDPMEERKRVAENFAIGKRYLSYEVLR
                     RRLPTLFMNFGAVS"
BASE COUNT          259 a          604 c          617 g          327 t
ORIGIN      
        1 cagggtcagc cccggggccg gcgtcgagtc cgaacgcggc gagtacgcct tctcggtctc
       61 gctccgggtc gactcgagcc tggtcatcgt gccgaccggc tctttcgggg ccatgttcgg
      121 ctcggcgcga gcacccgacg cgctccctcc ggcggtatcg tccttcaggg ccgaggcgag
      181 cgcctacgtg gcggggagct tcggtagcgc ctcgcttacg ggcggcaccg ccgtgtcggt
      241 ctcggcggcc atggccccga atctcgacgt cggggcgggc atggagataa acgaattcga
      301 atcggtggcc gggaggctcg gcgcgcgact atcgacgagg ctcggcgacg gcttggcggc
      361 ggcggtcctg gtcgacggca ggatagccga gggtacggtg ggagagccgg cctgggcccg
      421 actcggcgcg gccctcggcg tcggcacgcc cttcctgaac gcggtggcca tgccggcctt
      481 cgcggcgtac tgggaggacg gctgctcgct cagggcgggg ctcggggcgg cgttcaacct
      541 gtccggctac gccttcggcg cgtcgctctc ggcctccgcc ctgaccggcc cgctatcggg
      601 cggcttggag ctcgactggc ccatcggtgt gacgctggag ctcagattcc tgccgccagg
      661 cctccccctc gcgttcaggc tctcgggcgg cgtcgactgg tcgccggctc cgaccggctg
      721 gaacctcggc ctcgccgtct cgggtgggtt ctagcggcgc tcaggatacg gcgccgaagt
      781 tcatgaacag cgtcggcagg cggcgcctga gcacctcgta ggacaggtag cgcttgccga
      841 tcgcgaagtt ctcggcgacg cgcttccgct cctccatcgg gtcggatagc atacgggcca
      901 cgtccttgac gaccgcgtcg gtcacgtagc cgtccatcac cgtcgtcctg aagcccaggg
      961 gctcgatgtc ctgcgcgtaa atcgcatagc ggttgaccag tatgggcttc cggtagaaga
     1021 tcgcctcgag gaaggcgttg ccgaagccct cgtaggtcga cgggtacgtg acgaaatctg
     1081 cgcgcacata ggtatcccag agcgagtaca ccttgccgcc gtcctgccgc gtcccccggt
     1141 tcacgtcgat caggtcgggc cgcacgatca gctcgacgtc catgtacttt gcgtagtcga
     1201 gcacgcggtg ctggtactcg tcgccctcgt cgaggtccgg gtgcgatatc aggaggcgcc
     1261 cccgctggtt cggaccgacc gtgccgttgt acctggcgac cagctcgatg gcgctctcga
     1321 tgcccttacg ggccacgatc ctggtcggct gaaggaacag cacctcctcg tctcccatgc
     1381 ccagctcgga gcggagaccg gcgttgtatt cgtcggcctc ggtcggctcc gcatcgaagt
     1441 cccagacgtt cgggatcatc accgacgaga tgccgcaccg gaaggcgagc tgcctgcgcg
     1501 cttccgagtt gatgacgacg tgatagacgc tgtgcaggcg cggcgggaag gcgcggacta
     1561 tataatcctc cgcgcagccg gtcatgaagc gctggcgctc ccaggcgaag tcgtggttat
     1621 gcgccaccgt cggcatctcc agctcggcga cgagctccgc gagggcgatg ccgagcggta
     1681 tatgcatcgg tatcgcgaag gcgttctcga cgatcagcac atcgatgttg aattccttga
     1741 cgaactcgta cagcttggcc ttgagccggc cctttatctc gccgacctcc gcggtcaggc
     1801 ctctacg
//
LOCUS       DAOPHV010000567         1801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_93502_length_1801_cov_5.158076, whole genome shotgun sequence.
ACCESSION   DAOPHV010000567 DAOPHV010000000
VERSION     DAOPHV010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93502_length_1801_cov_5.158076"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PLQ29_12155"
     CDS             <1..322
                     /locus_tag="PLQ29_12155"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPG87440.1"
                     /translation="GPGEELVCVVTVGYAAAPGRPHRTKPIEAFVPGGIADKPAWFVS
                     GVEAAMLAPTALNQQKFSFALDGNVVDARVSGLGVLTDVDLGIARLHFELGAGAEAFV
                     WKHA"
     gene            551..751
                     /locus_tag="PLQ29_12160"
     CDS             551..751
                     /locus_tag="PLQ29_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPG87441.1"
                     /translation="MLLRRSMRDKKIAGVCGGMARSMGLSSTSVRWFFVLLVLLFGMS
                     GWVYLVLWAIIPSDEDHGEYKE"
     gene            complement(778..1263)
                     /locus_tag="PLQ29_12165"
     CDS             complement(778..1263)
                     /locus_tag="PLQ29_12165"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPG87442.1"
                     /translation="MERYHPRRARNEILSDDEKLSLLRGGDHVTLALCADDDPYVVTL
                     SYGLDWENRRLYFHCANKGDKLEYIRRNGRACATLIKDNGYLETRCDHDYESLVIRGP
                     IVVVDDLDEKKRALRVLLEHLERDPEPIFERNIKDDRSYDGVTILRLDMESIVGKKYL
                     G"
BASE COUNT          348 a          511 c          579 g          363 t
ORIGIN      
        1 cggcccgggc gaagagctcg tctgcgtcgt caccgtcggc tacgccgccg cgcccggcag
       61 gccgcaccgg accaagccta tcgaggcctt cgtcccgggc gggatagccg acaagcccgc
      121 ctggttcgtc tccggcgtcg aggccgccat gctcgccccg accgccctga accagcagaa
      181 gttctcgttc gccctggacg gcaacgtcgt cgacgcgcgg gtgtccggcc tcggcgtcct
      241 gaccgacgtc gacctcggca tagccaggct ccatttcgag ctcggcgccg gcgctgaggc
      301 cttcgtctgg aagcacgctt agcccgagaa ggtctcgatc ggggtcgaga aggtctcgat
      361 cggggtcgag aaggtctcga ttggggtacg gaaggtctcg attggggtcg ggaaggtctc
      421 gattggggtc gggaaggtct cgattggggt acggaaggtc tcgatcaggg tcgagaaggc
      481 cgttgacgcg gggcgtggcg gccgattagc ctgagatccg ggttaaaaaa cgattatcga
      541 ggagaacgac atgctactga ggcgatcgat gcgcgacaag aagatagccg gcgtctgcgg
      601 cggtatggcc aggtcgatgg gactgagctc cacgtcggtt cgctggttct tcgtgctgct
      661 cgtgctgctc ttcggcatgt cgggctgggt ctacctcgtc ctgtgggcga tcatcccgag
      721 cgacgaggac cacggcgagt acaaggagta gccggaacgg cgagccgccg cggccggcta
      781 cccgaggtat ttcttgccga cgatcgattc catgtcgagc ctcaggatag tcaccccgtc
      841 ataggatcgg tcgtccttga tattgcgctc gaagatgggc tcgggatcgc gctcgaggtg
      901 ttcgagcaag acccgtaggg cgcgcttctt ctcgtccagg tcgtcgacta cgactatcgg
      961 gccgcgaatg accagcgact cgtagtcgtg gtcgcagcgc gtctccaggt agccgttatc
     1021 cttgatcagc gtcgcgcagg cccggccgtt cctgcgtatg tactcgagct tatcgccctt
     1081 gttggcgcag tggaaataca gccgccggtt ctcccagtcg agtccgtagc tcagcgtgac
     1141 gacgtaggga tcgtcgtcgg cgcacagcgc cagcgtgacg tggtcgccgc cgcgcagcag
     1201 cgacagcttc tcgtcgtccg atagtatctc gttccgcgcc cgcctcgggt gatagcgttc
     1261 catggctgcc tccgcgtagc agtatagcat cccgagagta atgcgaggac tatctcctgg
     1321 cgctggcagt ccttgagcgg aacgaacccc ggataacctt ggccgagatg agaaaagaac
     1381 ttggcctgga acgttgaatt tattctgacg gctggaaggc aacttaggaa gcttgacaaa
     1441 aagtggcaag ctttacttat cgattatctg gaggatgaag tagcctcttc ggagaatccg
     1501 agaactagag gcaaggcgct gatgggagat aagaagggtc tctggcgcta tcggataggg
     1561 taaccgccaa tttcacccca tcttgcattc tgtttaaaag ctgataccgt tgcccgttca
     1621 aggagaactg agcaatggta ttcaccaaag acaggaagat attcatcaag acgggcttca
     1681 ccgacatgcg aaagcagatc aacggactcg cggctatcgc ggaaagctcg atgcccgagc
     1741 cgccgttatc gggcgcctat ttcgtgttct gcggaaagac ccgcaggttg atcaaagtgt
     1801 t
//
LOCUS       DAOPHV010000568         1799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_93681_length_1799_cov_2.768922, whole genome shotgun sequence.
ACCESSION   DAOPHV010000568 DAOPHV010000000
VERSION     DAOPHV010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93681_length_1799_cov_2.768922"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PLQ29_12170"
     CDS             <1..378
                     /locus_tag="PLQ29_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HPG87443.1"
                     /translation="AILRRQLTSDVFVSGSNAVTLDGEIVNVDGTGNRVAALSFGPAK
                     TVVVVGINKVVRDLDEAFARIETSAAPMNNKRLDRPNPCVKAGACMDCDGDTRICRIY
                     QILRKRPGGTDFTVIVVGEDLGY"
     gene            complement(413..>1799)
                     /locus_tag="PLQ29_12175"
     CDS             complement(413..>1799)
                     /locus_tag="PLQ29_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HPG87444.1"
                     /translation="DFASYDSLMPRADGVLFVADGQVMRLDRQRGAVTKAVSETNAVL
                     TAASAVGTQLESLYLPGAAGSPSPAMSLSELWLVAFHDDKPHREAADGRHGIYLQTGF
                     MVRADSRAKYDALLDERGLDTVVVDLKDDLGRLRFEPRDPLVKAIGRAVNPLDVEDFV
                     GEMKAKGRYLVARIVVFKDQRLHEYLGGAYAVWDRKENAPWRGYELETKAVPVPTPPG
                     APPSSAAPAMTSVTERRYYGEYWVDPYSEKVWEYNVAIAREIIARGFDEVQFDYIRFP
                     TDGANLGDASYRWKDPGMDMESALMSFLSYARRNIDAPISIDIYGANGWYRSGVRTGQ
                     DVELLARYVDAICPMFYPSHFEQGFMAHAPAELRPYRIYKIGTLRNSYIARKRVVVRP
                     YVQAFYLNVRYDREYYSPAYVALEVDGVRDASNEGLLYWNNSGRYDDIPTLKRGADRR
                     IVSKPERSVLD"
BASE COUNT          289 a          581 c          623 g          306 t
ORIGIN      
        1 gccatacttc ggcgccaact cacgagcgac gtcttcgtct ccgggtcgaa tgccgtgacg
       61 ctcgacggcg agatagtcaa cgtcgacggt acgggcaacc gcgtggcggc gctcagcttc
      121 ggtccggcca agacggtcgt ggtggtcggg atcaacaagg tcgtccgcga cctcgacgaa
      181 gccttcgccc gcatcgagac cagcgccgcc ccgatgaaca acaagcggct cgaccggccg
      241 aacccctgcg tcaaggcggg cgcctgcatg gactgcgacg gcgacacgag gatctgccgg
      301 atctaccaga tactgcgcaa gcggccgggc ggtaccgact tcacggtcat cgtcgtcggc
      361 gaggacctgg ggtactagga tgggccgggc ggggccgcga gtcggctccg cctcagtcca
      421 gcacgctccg ttccggcttg gagacgatgc gccggtcggc gccccgcttc agcgtcggta
      481 tatcgtcgta ccttccggag ttattccagt acaacaggcc ctcgttcgac gcgtcgcgta
      541 cgccgtccac ctcgagcgcc acgtaggccg gtgagtagta ctcgcggtcg tagcgcacgt
      601 tcaggtagaa ggcctgcacg tagggccgga cgaccacgcg cttcctggcg atgtacgagt
      661 tgcgtagcgt gcctatctta tagatgcggt aggggcgtag ctccgccggc gcgtgggcca
      721 tgaagccctg ctcgaagtgc gagggataga acatcgggca gatcgcgtcg acgtagcggg
      781 cgagcagctc gacgtcctgg ccggtgcgga cgccggagcg gtaccagccg ttggcgccgt
      841 agatgtcgat gctgatcggc gcgtctatgt tgcgcctggc gtaggacagg aaggacatga
      901 gcgcgctctc catgtccatg cccgggtcct tccagcggta gctggcgtcg ccgaggttgg
      961 cgccatcggt cgggaagcgt atgtaatcga actggacctc gtcgaagccc cgggcgataa
     1021 tctcccgggc tatggctacg ttgtactccc agaccttctc ggagtacgga tcgacccagt
     1081 attcgccgta atacctgcgc tcggtgacgc tggtcatcgc cggcgccgcg gaggacggcg
     1141 gggccccagg cggcgtcggt accgggaccg ccttcgtttc aagctcgtag ccgcgccagg
     1201 gggcgttttc cttcctgtcc cagaccgcgt aggcgccgcc caggtactcg tggaggcgct
     1261 ggtccttgaa gacgacgatg cgggcgacga ggtagcggcc cttggccttc atctccccga
     1321 cgaagtcctc cacgtcgagc gggttgaccg cgcggcctat cgccttgacc aagggatcgc
     1381 gcggttcgaa gcgcaggcgc cccagatcgt ccttgaggtc gacgacgacc gtgtcgaggc
     1441 cgcgctcgtc gagcaacgcg tcgtacttgg cgcggctgtc ggcgcggacc atgaagccgg
     1501 tctgcaggta gatgccgtgg cggccgtcgg cggcctcgcg gtggggcttg tcgtcatgga
     1561 aagcgacgag ccacagttca gaaaggctca tcgccggcga cggggagcct gccgcgccgg
     1621 gcaggtagag cgactcgagt tgcgtcccga cggccgaggc ggccgtcagg acggcgttcg
     1681 tctcgctgac ggccttggtc acggcgcctc gctgcctgtc caggcgcatc acctggccgt
     1741 cggctacgaa cagcacgccg tcggctcgcg gcatcagcga gtcgtacgaa gcgaaatcc
//
LOCUS       DAOPHV010000569         1797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_93803_length_1797_cov_4.045350, whole genome shotgun sequence.
ACCESSION   DAOPHV010000569 DAOPHV010000000
VERSION     DAOPHV010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93803_length_1797_cov_4.045350"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLQ29_12180"
     CDS             complement(<1..251)
                     /locus_tag="PLQ29_12180"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPG87445.1"
                     /translation="MSRIAVRRFGILTSGGDCPGLNAAIRGVAKEASGVYGMEIVGIS
                     NGYRGLIEGDARDLAPDDFSGILTRGGTILGSSREKPFKP"
     gene            complement(293..931)
                     /locus_tag="PLQ29_12185"
     CDS             complement(293..931)
                     /locus_tag="PLQ29_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87446.1"
                     /translation="MKKTTLAVPAGTERVKAFAAEAKVSGRAVASLRLVEPGEPASGD
                     EPGDGADVIDWNPLSFASARAAVLEAETAFGGRLDELVIFADPPADAAGIVDQTPASI
                     ERAAMEWAAGYAELIREAAKRLDERGGGTIALIVAQAERGPIGSMAAGALLGLAEGLV
                     RSAPGKVRFVGAKDESDQADLLARQVVKTLDEEPRETRRLLRLGGRGSLFSR"
     gene            1006..>1797
                     /locus_tag="PLQ29_12190"
     CDS             1006..>1797
                     /locus_tag="PLQ29_12190"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPG87447.1"
                     /translation="MKLALCQIDAVIGDFDGNVERIAAAARVAASSPTPPDLVVFPEM
                     SLCGYPPMDLLDQPSFAERDEAAFRRLQKALPRGVAVAVGHIGRNRSGLGRPLRNALS
                     VVLDGEVVFEQAKTLLPTYDVFDEARYFEPASERRLWRHRGVAVGFAICEDLWRETAP
                     VPGMRYPVDPPRELLDAGADLIVVPSASPFQLDKSALRVGLCSDLARTGSVPVAYCNM
                     AGADDSLVFDGRSFLVDRTGRLVALGVFGEGILAVDTDECADEATI"
BASE COUNT          235 a          658 c          601 g          303 t
ORIGIN      
        1 ggcttgaacg gcttctcgcg ggacgaaccg agtatggtgc cgcctcgcgt caggatgccc
       61 gagaagtcgt ccggcgccag gtctcgggcg tcgccttcga tcaggcctcg gtaaccgttc
      121 gatatcccga ctatttccat gccgtagacg ccggaggcct ccttggcgac gccgcggatc
      181 gcggcgttca ggccggggca atcgcccccg ctcgtcagta tcccgaagcg ccgtaccgcg
      241 atcctcgaca tggcttcctc ctcgatacgg ccccgacggc tgcgcgttcg ggtcagcggc
      301 tgaacaggct ccctcggccg ccgaggcgga gtaggcggcg cgtctcgcga ggctcctcgt
      361 cgagcgtctt gaccacctgt ctggccagga ggtccgcctg gtcggactcg tccttggcgc
      421 cgacgaagcg caccttgccc ggcgcggaac gtacgagacc ctcggccagc ccgagcaggg
      481 ccccggccgc catcgagcct atcgggccgc gctcggcctg cgcgacgatg agggctatcg
      541 tccccccgcc gcgctcatcg aggcgcttgg cggcctcgcg gatgagctcg gcgtagccgg
      601 cggcccactc catggcggct cgctctatgg acgccggtgt ctggtcgact atgccggcgg
      661 cgtcggccgg cgggtcggcg aagatcacca gctcgtcgag cctgcccccg aaggccgtct
      721 ccgcctcgag cacggccgcc ctggccgaag cgaacgacag cgggttccag tcgatcacgt
      781 ccgcgccgtc tcccggctcg tcgccggagg ccggttcgcc aggctcgacg agccgcaggg
      841 acgcgacggc tcgcccggat accttggcct cggcggcgaa cgccttgacg cgctccgtcc
      901 ccgccgggac cgccaacgtt gtctttttca cgggcgcagt gtactatggg cggcgagcgg
      961 acgcaaggta gcgtcttcgt ccgagccgcc ttgaacggag ttcccatgaa gcttgcgcta
     1021 tgccagatcg acgcggtcat cggcgacttc gacggcaacg tcgagcgcat cgccgcggcc
     1081 gcgcgggtcg ccgcctcgtc cccgacgccg ccggacctcg tcgtattccc cgagatgagc
     1141 ctttgcggct acccgccgat ggatttgctc gatcagccga gcttcgccga acgcgacgag
     1201 gcggcctttc gccggctcca gaaggcgttg ccgcggggcg tcgcggtggc tgtcggccat
     1261 ataggccgca accgctcggg cctcggcagg ccgctgcgca acgcgctgtc ggtcgtcctc
     1321 gacggcgagg tggtcttcga gcaggccaag accctgctgc ccacctatga cgtattcgac
     1381 gaggcgcgct atttcgagcc ggcctccgag cgtcggctct ggcgccatcg cggcgtcgcg
     1441 gtcggtttcg ccatctgcga ggacctatgg cgggagaccg ccccggtccc cggtatgcgc
     1501 taccctgtgg atccgccgcg agagctgctc gacgccggcg ccgacctgat cgtcgtgccg
     1561 tccgcctctc cgttccagct ggataagtcg gccctgcgcg tcggactctg ctccgacctg
     1621 gccaggacag gctcggttcc ggtcgcctac tgcaacatgg ccggtgccga cgactcgctc
     1681 gtcttcgacg gccggtcctt cctcgtcgac cgcacaggcc gcctcgtcgc gctcggggtc
     1741 ttcggtgagg gcatactcgc cgtcgatacc gacgaatgtg ccgacgaggc cacgatc
//
LOCUS       DAOPHV010000570         1797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_93813_length_1797_cov_3.491963, whole genome shotgun sequence.
ACCESSION   DAOPHV010000570 DAOPHV010000000
VERSION     DAOPHV010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93813_length_1797_cov_3.491963"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLQ29_12195"
     CDS             complement(<1..413)
                     /locus_tag="PLQ29_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87448.1"
                     /translation="MDASPSDNTVLEANMRALRSRSTALAELVAAAEPYPLEPVVARS
                     GDPTATVAGAWLHSRYDPRAEGDRIAAEAAASGASTLLFLGLGLGYAAKAAVDSGATV
                     CAVESDASLLAASLRLVDLSALIASPRFSIALCPRG"
     gene            complement(413..>1797)
                     /locus_tag="PLQ29_12200"
     CDS             complement(413..>1797)
                     /locus_tag="PLQ29_12200"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HPG87449.1"
                     /translation="GLSGAEAAFDSWLSADPKEAGGGYAYGDAVYLTIDADLQYRLET
                     LSRAVLEENAAEALALVAMEAKTGDILAYVSLPDFDPNDFMAVDRSAWTDRVAIYAYE
                     PGSVFKVYSMASLMALGGIDERSRFFCDGSYEKVTASGEEIVIKCLGHHGNVDVTNIL
                     EYSCNAGAAYASETVSDIDFYAKIREFGFGERVGADLTGESPGLMRRPGDWSGRTKPT
                     MAMGQELLVTALQMADAATVVANGGVLMRARTLGRIVSADGIVLEEPQPIAVRRVMGE
                     YEAKAILRSMEAAVLDSGTGRRARIDDLRMSVKTGTAQVIDPRTRRYSDTDFIASTIA
                     LFPSEDPEYIVYAAIFNPKGASTYGGRIAAPFVKDAANAIADLYGVARSGSASATHGG
                     RIAVSGVEAAEVGDAMPDLRGLPKRALTGLLARDDLVVEIEGDGWVREQSPAPGEPIA
                     AGAVIRLRLE"
BASE COUNT          295 a          619 c          589 g          294 t
ORIGIN      
        1 cctcgagggc acaacgcgat cgagaagcgg ggcgatgcta taagcgccga caggtcgacc
       61 aggcggagcg aggcggccag caaggacgcg tccgattcca ccgcgcatac cgtcgcgccg
      121 gagtcgacgg ccgccttggc cgcgtagccg agccccaggc cgaggaacag cagggtcgag
      181 gcgccgctcg cggcggcttc agccgctatc ctatcgccct cggcgcgtgg atcgtagcga
      241 gagtgtagcc aggcgccggc aaccgtggct gtcggatcgc cgctccgggc gacgactggc
      301 tcgagcgggt acggctcggc cgccgcgacg agctccgcca aggccgtcga gcggctcctc
      361 agggcgcgca tattcgcctc gaggacggtg ttgtcggacg gcgacgcgtc catcactcca
      421 gcctcaacct gatgacggct ccggccgcta taggctcccc gggcgccggc gactgctcgc
      481 gaacccatcc gtcgccctct atctcgacga cgaggtcatc ccgggcgagc agtccggtca
      541 gcgcccgctt gggcaggcct cgcaaatcgg gcatcgcgtc gcctacctca gccgcctcga
      601 ctccgctgac cgctatccga ccgccatggg tcgcagaggc cgaaccggag cgggccacgc
      661 cgtagaggtc ggcgatcgcg ttcgccgcgt ccttgacgaa gggggcggca atacgtccgc
      721 cgtacgtcga ggcgcctttc gggttgaaga tggcggcata cacgatgtac tcggggtcct
      781 cgctcgggaa gagcgctatt gtgctcgcga tgaagtcagt atccgaatag cggcgcgtcc
      841 tcgggtcgat gacctgggcc gtacctgtct tcacagacat gcggaggtcg tcgatgcggg
      901 cgcggcggcc ggtaccggag tcgagcacgg ccgcctccat cgagcgcagt atcgccttcg
      961 cctcgtactc gcccataacc ctacgtaccg ctatcggctg cggctcctcg aggacgatac
     1021 cgtctgccga tacgatgcga ccgagcgtcc tagcgcgcat cagcacgcca ccgttcgcca
     1081 cgacagtcgc cgcgtcggcc atctgcagcg ccgtgacgag cagctcctgt cccatcgcca
     1141 tggtcggctt cgtcctgccg gaccagtccc ccggccgcct catgagcccg gggctctcgc
     1201 ctgtcaggtc ggcgccgacg cgctcaccga aaccgaattc ccgtatcttg gcatagaagt
     1261 ctatatccga taccgtctcg gaggcgtagg cggcgccggc gttgcacgag tactcgagaa
     1321 tgttagtcac gtcgacgttg ccgtggtggc cgaggcactt gatgacgatc tcctcgccgg
     1381 atgccgtcac cttctcgtag cttccgtcgc agaagaaacg cgagcgctcg tcgatgccgc
     1441 cgagggccat caacgaggcc atgctgtata ccttgaagac cgatcccggc tcgtaggcgt
     1501 agatggccac gcggtcggtc cacgccgacc gatcgaccgc catgaaatcg ttcgggtcga
     1561 aatccggcag ggatacgtac gccaggatat cgccggtctt cgcttccatc gcgacgaggg
     1621 ccagggcttc ggcggcgttc tcctcgagaa cggcgcggga aagcgtctcg aggcggtact
     1681 ggagatcggc gtcgatggtc aggtagacgg cgtcgccgta cgcatagcct cctccggcct
     1741 ccttcgggtc ggccgacagc catgagtcga aggccgcctc ggcgccggac aagccct
//
LOCUS       DAOPHV010000571         1793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_94103_length_1793_cov_4.220368, whole genome shotgun sequence.
ACCESSION   DAOPHV010000571 DAOPHV010000000
VERSION     DAOPHV010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94103_length_1793_cov_4.220368"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..748)
                     /locus_tag="PLQ29_12205"
     CDS             complement(209..748)
                     /locus_tag="PLQ29_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG87450.1"
                     /translation="MSDIKEIRVDENSPLFDKTGMLYKEFPSDYRQIRYFTLLIVQSA
                     PLEIKEINLLEQQISEVIKNGVKHGNRCEASKKVRVWYSFDSTHAHLIVQDEGEGFKD
                     LEKWNEFNRNRLEILSAQDFEKLSDYVSFRTQKSDDNDGGNALFAALEYWNGGFVFNE
                     ARNAVAMLKSFPQKKHAIG"
     gene            complement(776..1114)
                     /locus_tag="PLQ29_12210"
     CDS             complement(776..1114)
                     /locus_tag="PLQ29_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521480.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HPG87451.1"
                     /translation="MELKIRKNGNNYIIDVNGEMDLYNSYKLKELVMKMLEKKVERFI
                     INLENVDYIDSSGIGALIYICSTIKKMSLKLIITNIHGSVKKVIELTKLMGYFPITNS
                     IEEAILQMED"
     gene            complement(1130..>1793)
                     /locus_tag="PLQ29_12215"
     CDS             complement(1130..>1793)
                     /locus_tag="PLQ29_12215"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPG87452.1"
                     /translation="FPSLAIGAFTSPARGVYGDYYDVLRTREDRVVGVIGDVAGKGVQ
                     AALVMVMIRAILHLITNTNKDIATVLDWVNRGITGKIDMDHYATLAIVALNVATGELE
                     YANAAHQPLLVYRRSTDTIETIETKSVPIGVERANEYRRKALRLYDGDIAVLYTDGIV
                     EAMNEQGKQFGRKSLGQAVARNRDLAPKEIAGKIKSELSAFIGGARQHDDQTILVFKM
                     KL"
BASE COUNT          306 a          545 c          510 g          432 t
ORIGIN      
        1 tacttcggca tcgagttccg catgatggcg cccgaggact tcaggttcct gttcgagtac
       61 ctgtacggcc gtcccttcac cgacgaggac gccgattcgg tcgagggtat acgcatcgtc
      121 aacccgtagc aggccacgaa gaccccgcgc cataggaaga ggcggcccgc gagggccgcc
      181 tttccgtcgt cacggtggga aaccgggctc agcctatggc gtgcttcttt tgcgggaagc
      241 tcttgagcat cgctacggcg ttcctcgcct cgttgaacac gaagccgccg ttccagtact
      301 cgagggcggc gaacagcgcg ttgccgccgt cgttgtcatc gctcttctgc gtcctgaacg
      361 atacgtagtc cgacagcttc tcgaaatcct gggcggacag gatctcgagg cgatttcggt
      421 tgaactcgtt ccacttctcc agatccttga agccctcgcc ctcgtcctgg acgatcaggt
      481 gggcgtgggt cgagtcgaag gagtaccaga ccctcacctt cttcgaggcc tcgcaacgat
      541 tgccgtgctt gacgccgttc ttgatcacct cgctgatctg ctgctcgagc aggtttatct
      601 ccttgatctc gagcggggcc gactgcacga tcaggagcgt gaagtacctg atctgccggt
      661 agtccgaggg gaactccttg tagagcatcc cggtcttatc gaacaggggg ctattctcat
      721 cgacgcggat ttccttgatg tcgctcaggt acgtcttgtc agccatgccg ccctcctagt
      781 cttccatctg caggatggct tcctcgatgc tgttcgttat cgggaagtag cccatcagct
      841 tggtcagctc gatcactttc ttgaccgaac cgtggatgtt ggtgatgatc agctttaggc
      901 tcatcttctt aatggtagag caaatataga tgagggcacc tatcccgctc gagtcgatgt
      961 agtccacgtt ctccaggttg atgataaagc gctcgacctt cttctcgagc atcttcatga
     1021 cgagctcctt gagcttgtac gagttgtaaa gatccatctc cccgttgacg tcaataatgt
     1081 aattattgcc gttcttacgg atcttgagct ccatgcctca gcccctcccc tatagtttca
     1141 tcttgaagac gagtatcgtc tggtcgtcat gctgcctggc cccgccgatg aacgccgaca
     1201 gctccgactt aatcttaccg gctatttcct tgggcgccag gtcgcggttc ctggccacgg
     1261 cctgaccgag gctcttgcgg ccgaactgct tgccctgctc gttcatcgcc tcgacgatac
     1321 cgtcggtgta cagcacggcg atgtcgccgt catagagcct gagggccttg cgcctgtatt
     1381 cgttagctcg ctccacgcct atcggcacgc tcttcgtctc gatggtctcg atcgtatcgg
     1441 tcgagcggcg gtacacgagc agcggctgat gggccgcgtt ggcgtactcg agctcgcccg
     1501 tagccacgtt gagcgccacg atagcgagcg tcgcgtagtg gtccatgtcg atcttgccgg
     1561 tgatgccgcg gttgacccag tcgagcacgg tagcgatgtc cttgttcgtg ttcgtgatca
     1621 ggtggagtat cgcgcgtatc atcaccatga cgagggccgc ctggacgcct ttgcctgcca
     1681 cgtcgcctat cacgcctact accctgtcct cgcgggtacg tagcacgtcg tagtagtcgc
     1741 cgtacacgcc gcgggccggt gaggtgaacg cgccgatggc gagcgacggg aac
//
LOCUS       DAOPHV010000572         1786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_94658_length_1786_cov_3.093010, whole genome shotgun sequence.
ACCESSION   DAOPHV010000572 DAOPHV010000000
VERSION     DAOPHV010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94658_length_1786_cov_3.093010"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PLQ29_12220"
     CDS             <1..590
                     /locus_tag="PLQ29_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87453.1"
                     /translation="AYAGWTTFANVRKFRLCLKPGVETVTGSALSLEPGPRGGVGLRL
                     LYDNHATDPDFAYTDRGQAAALGSGVITVQPVWLENPAGLSYDVHSLVVNENLQALYD
                     PLYSSAAEYWPDAWTGSWTVDEIRSATGKSVAGSKVALVRQVTNLSAVNPLGDGNSSY
                     RFSYPAYVGKYLVVALVVTDGQLSVPTQAVVIPIE"
     gene            complement(662..>1786)
                     /locus_tag="PLQ29_12225"
     CDS             complement(662..>1786)
                     /locus_tag="PLQ29_12225"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HPG87454.1"
                     /translation="RGARDPASLASHVIPSASSSLGVWRTGSDDEMDMGDRGNLTAFA
                     AWATEAYPEYRRHALILWNHGSGLAASRSIGFDDEVYVDDGRYGSAGQLYIGELDEAL
                     EPVYGPGGLAGHRLEAFGADACHLGLYEAAYELRELAGYFAASPATEYGGWNYARLFS
                     TEAAFDSGRAFVREVVQGYEAKSTFYPNTMTALDLAGMESLKTAVDGFGAALLDYRDG
                     GADEAERSVRQAAIEALRNASVAYYSGSQYDLPLYAPYHDLGDFSERVNKAALPADAT
                     AAAEAVLEALSSAVVYTWTASEYGGFKGACGRGLGIFFSHGDETHTLNGQARSHYAYQ
                     GYYTDGYSGIQDIPYGYLEAADQGAGSTTWRGMLDAFYGQ"
BASE COUNT          282 a          603 c          600 g          301 t
ORIGIN      
        1 cggcctacgc tggctggacc accttcgcca acgtacgcaa gttcagactc tgcctcaagc
       61 ccggcgtcga gactgtcacc ggctccgctc tgtcgctcga gcccggccct cgcggcggcg
      121 tcggcctcag gctgctctac gataaccacg cgaccgaccc cgacttcgcc tacaccgacc
      181 gcggccaggc cgcggctctc ggctccggcg tcataaccgt gcaacctgtc tggctcgaga
      241 accccgcggg ccttagctac gacgtgcaca gcctcgtcgt gaacgagaac ctccaggcgc
      301 tctacgaccc cctgtacagc agcgccgctg agtattggcc ggacgcctgg accggttcgt
      361 ggaccgtgga cgagatacgt tcagcgacgg gcaagtcagt agccgggtcg aaagtggcgc
      421 tcgtccgcca ggtgaccaac ctgagcgccg tgaatccact cggcgacggc aacagctcgt
      481 accgcttctc gtaccccgcc tacgtcggca agtacctcgt ggtggccctg gtcgtcaccg
      541 acgggcagct gtcggtaccg acccaggcgg tggtcatacc gatagagtga tcgcgacgag
      601 tacgtcgcga tggcgcggga atgaagcggc ggctgggtcc gatggagcca gccgccttct
      661 tttattggcc gtagaacgcg tcgagcatgc cgcgccaggt ggtcgatccg gcgccttggt
      721 cggccgcctc gaggtacccg tagggaatat cctggatacc gctgtagccg tcggtgtagt
      781 agccctggta ggcgtagtgg ctgcgcgcct gtccgttcag cgtatgggtc tcgtcgccgt
      841 ggctgaagaa gatgcccagt ccgcggccgc aagcgccctt gaagccgccg tactcgctcg
      901 cggtccaggt gtagacgacg gccgaggaca gggcctccag cactgcctcg gcggcggcgg
      961 tggcgtcagc tggtaaggcg gccttattca cgcgttccga gaagtcgccg aggtcgtggt
     1021 agggggcgta gagcggcagg tcgtactgag agccggaata ataggcgacg ctcgcgttac
     1081 ggagcgcttc gatcgcggct tggcgaacgg atcgctccgc ttcgtcggcg ccaccgtcgc
     1141 gatagtcgag cagggccgct ccgaacccgt cgaccgccgt cttgagcgac tccatgcccg
     1201 ctaggtccag cgccgtcatc gtgttcggat agaacgtcga cttggcctcg tagccctgga
     1261 cgacctccct gacgaaggcg cggccggaat cgaaggcggc ttcggtggag aagaggcggg
     1321 cgtagttcca gcctccgtac tcggtggccg gcgaggccgc gaaatagccg gccagctcgc
     1381 gtagctcgta ggccgcctcg tagaggccca ggtggcaggc gtcggcgccg aacgcctcga
     1441 gccggtggcc ggccaggcct cccgggccgt agaccggctc gagcgcctcg tcaagctcgc
     1501 ctatgtatag ttggcccgcc gagccgtagc ggccgtcgtc aacgtaaacc tcgtcgtcga
     1561 aaccgatcga gcgagacgcc gcgagcccgc tgccgtggtt ccatagtatc agcgcgtgcc
     1621 gcctatactc ggggtaggct tccgtcgccc aggccgcgaa ggccgtcagg ttgccgcggt
     1681 cgcccatatc catctcgtcg tcgcttccgg tccgccagac tccgaggctc gacgaggcgc
     1741 tcgggatcac gtgggaggcc aggctggccg ggtcccgagc gcctcg
//
LOCUS       DAOPHV010000573         1780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_95092_length_1780_cov_6.194203, whole genome shotgun sequence.
ACCESSION   DAOPHV010000573 DAOPHV010000000
VERSION     DAOPHV010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95092_length_1780_cov_6.194203"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..972)
                     /locus_tag="PLQ29_12230"
     CDS             complement(115..972)
                     /locus_tag="PLQ29_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG87455.1"
                     /translation="MIEVERLCHDYEGKGVLAVDDVSFRAEPGTIYGFLGPSGAGKST
                     VQNILTGLLPLQRGSAALLGQAVTSLRPSFFNRVGYSFELPNLFGALTGYENLAYFAG
                     LFAVPTEDPMRLLERVGLAAAAGKRVSTYSKGMKQRLLFLRAILNRPEVLFLDEPESG
                     LDPTTSLVIKDMIREQRDRGATILLTTHNMYAADELCDRVAFMDKGRILAEGRPRELK
                     LRYGERVAAVEYRDGNELRSERIALDGSAGRARLAELASSGRIETMHSMEASLEAIFI
                     RLTGRKLSA"
     gene            complement(976..1614)
                     /locus_tag="PLQ29_12235"
     CDS             complement(976..1614)
                     /locus_tag="PLQ29_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPG87456.1"
                     /translation="MTNGDDLASASFRQLSEERQNSILSSARTEFATKGFARASTNAI
                     VQACGIAKGSLFNYFDGKDGLFLYLHWLSGKRQLTALRSRSATHGTTQSAMDGLVRAV
                     ELAFDLFADAPDEWSFAMTMAGADAAHLVPRYVALFDAAESAALFRAVVTPPGTDAAV
                     AQALQWMLTGIKMEIQAGLRPASDTESRAETLRGLRSSILGRLEQIRGVIKE"
BASE COUNT          294 a          598 c          558 g          330 t
ORIGIN      
        1 tgagcacgcc caccgcgccg atggcgatgg ctaccagcac gatggccagc acgagcgaca
       61 ccccgttccg cgccatgacg acgagctcct gcctgagtag cgtgatgaag gccttcatgc
      121 ggacagcttc ctgcccgtca gccggatgaa gatggcttcc aggctcgctt ccatcgagtg
      181 catcgtctcg atccggcctg aggacgccag ctcggccagc ctggctcggc cggccgagcc
      241 gtcgagcgct atccgctcgg atcgtaattc gttcccgtcc cggtactcca ccgcggccac
      301 tcgctcgccg tagcgcagct tcagctcgcg cgggcgacct tcggcgagga tcctgccctt
      361 gtccatgaac gcgaccctgt cgcatagctc gtcggcggcg tacatgttgt gcgtcgtcaa
      421 gagtatcgtg gcgccacggt cgcgctgctc ccgtatcatg tccttgatga cgagcgacgt
      481 ggtcgggtcc agcccggact cgggctcgtc caagaaaagc acctccggtc ggttcaggat
      541 ggctcggagg aataggagcc gctgtttcat gcccttggaa tacgtgctga cgcgcttccc
      601 ggccgccgcc gcgagaccca cccgctcgag caggcgcatc ggatcctcgg tcggtaccgc
      661 gaacaggccg gcgaaatacg ccagattctc gtaaccggtt agcgcgccga acaggttggg
      721 tagctcgaac gagtagccca cccggttgaa gaacgacggc cgtagcgatg tcaccgcttg
      781 gccgagcagg gccgcggagc cgcgctgcag cggcaggagg cccgtcagga tgttctgcac
      841 ggtgctcttg ccggcgcccg acggtcccag gaagccgtag atggtaccgg gctccgcgcg
      901 gaagctcacg tcgtcgaccg ccagtacgcc cttgccttcg taatcatggc acaggcgctc
      961 cacttctatc atgcgctact ccttgatgac gccgcggatt tgttccagcc gtccaagtat
     1021 ggacgacctg agtccgcgga gcgtctcggc ccgcgattcg gtatcggagg cgggtcgtag
     1081 tccggcctgg atctccatct tgatgccggt cagcatccac tgcagcgcct gcgcgaccgc
     1141 ggcgtcggtg cccggcggcg taacgacggc cctgaacaac gcggcgctct ctgccgcgtc
     1201 gaacagcgct acgtagcgcg gcaccaggtg cgccgcgtcc gcgcccgcca tcgtcatcgc
     1261 gaaggaccac tcgtccggcg cgtcggcgaa caggtcgaac gccagctcga cggctcgcac
     1321 gaggccgtcc atcgcgctct gcgtcgtgcc gtgggttgcg ctacgagaac gcagggccgt
     1381 cagttgccgc ttgcccgaca accagtgcag gtataggaac agcccgtcct tgccgtcgaa
     1441 atagttgaag agcgagcctt tggcgatgcc gcacgcttgt acgatggcgt tggtcgacgc
     1501 gcgtgcgaag cccttggtcg cgaactccgt tcgcgccgag cttaggatcg aattctggcg
     1561 ctcttccgac agttgcctga acgaagcgct cgctagatcg tcgccgttcg tcatcctcgc
     1621 tcctcttgac cgcccggtca aatatgacga tagcgcatat tgaccacgtg gtcaagttcg
     1681 atcgcgctgg ttccgcaacc tggggatagg tctcccgctt gacgagcctt gacgccacgc
     1741 ggctagccgt gcgacagtgc ggtttgtgtt aaccattgaa
//
LOCUS       DAOPHV010000574         1780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_95122_length_1780_cov_3.093913, whole genome shotgun sequence.
ACCESSION   DAOPHV010000574 DAOPHV010000000
VERSION     DAOPHV010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95122_length_1780_cov_3.093913"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="PLQ29_12240"
     CDS             complement(<1..142)
                     /locus_tag="PLQ29_12240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HPG87457.1"
                     /translation="MTTGNKVYRCAKCGNVVELLHEGGGELVCCGEPMKLLTENTSDG
                     AKE"
     gene            complement(154..729)
                     /locus_tag="PLQ29_12245"
     CDS             complement(154..729)
                     /locus_tag="PLQ29_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPG87458.1"
                     /translation="MKSVKGTQTEKNLMEAFAGESQARNRYTYFASKARDEGYMQIAA
                     VFEETANQEKEHAKRLFKLMDGGVISINGTFPAGPVGTTSENLLEAAKGEDHEWQEMY
                     PGFAKTARAEGFDAIATIFEAIAVAERQHSKRYSGLKANVDAGVAFKRTAPVVWQCRN
                     CGYIVEAVSAPQACPACAHPQSYFELLGENW"
     gene            901..1599
                     /gene="radC"
                     /locus_tag="PLQ29_12250"
     CDS             901..1599
                     /gene="radC"
                     /locus_tag="PLQ29_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPG87459.1"
                     /translation="MKHAIESYCWAGEASGQKPDIRERLIASGTPSVADYELVAAIIG
                     AGSRGKDVRTLSTEVLAGCDFSAGVPSVSSISSIPGMGEARACRVAAAFELGKRFYGT
                     RERRIASPNDAWQLVRHFDDRKQERFLCCTLNGAHDVIAVRVITVGLVNRTIVHPREI
                     YAEAVADRACAIFVAHNHPSGRLEPSPEDLDITTRLKEAGLVLGIPLLDHLVFSHAGY
                     TSMVESGLLVPDKA"
BASE COUNT          325 a          593 c          518 g          344 t
ORIGIN      
        1 tctccttggc gccatcgctc gtgttctcgg taaggagctt catcggctcg ccacagcaga
       61 cgagctcgcc gccgccctcg tggagcagtt ccacgacgtt accgcacttg gcgcaacggt
      121 acaccttgtt tcccgtggtc atgatgtccc tccttaccaa ttctcgccga gtagctcgaa
      181 atacgactgg ggatgagcgc aggccgggca ggcctggggt gccgatacgg cctcgactat
      241 gtagccacaa ttcctgcact gccagacgac gggggccgta cgcttgaagg cgacgcccgc
      301 gtcgacgttc gccttgagcc ccgaatagcg cttggaatgc tgccgctcgg ccacggcgat
      361 cgcctcgaag atggtagcga tggcgtcgaa gccctcggct cgggccgtct tggcgaagcc
      421 gggatacatc tcctgccact catggtcctc gcccttcgcc gcctcaagga ggttctccga
      481 ggtcgttccg accgggccag cggggaaggt gccgttgatg cttatgactc ccccatccat
      541 cagcttgaac agccgcttcg cgtgctcctt ctcctggttc gcggtctcct cgaacacggc
      601 ggctatctgc atatagccct cgtcgcgcgc cttgctcgcg aagtacgtat agcgattcct
      661 cgcctgcgac tcgccggcga aagcctccat caggttcttt tctgtctggg ttcccttaac
      721 gcttttcatc ttgcccccga tcctcatgaa ttcctcccgg ccaacgccgg gacgcataca
      781 ccagcgattc gaaagtatac aatcaaatat cgctggaagc aaatagagca tacagtttta
      841 gtcatgtcct agatcgaaag cgccgcggct ccgccgagcg taccggccgc ggaggcatcg
      901 atgaaacacg cgatcgagtc atactgttgg gcgggcgagg cgtcgggcca aaaaccggat
      961 atcagggaga ggctcatagc atcgggaact ccgtccgtcg ccgactacga actggtcgcc
     1021 gccatcatcg gcgccggttc gagaggcaag gacgtgcgaa cgctatcgac ggaggtgctc
     1081 gccggatgcg acttcagcgc cggcgtcccg agcgtctcga gcatatcgtc gatcccgggc
     1141 atgggcgaag cgcgagcctg ccgcgtagcc gcggctttcg agctcggtaa gcgcttctat
     1201 ggaacgaggg aacgcaggat cgcgtccccg aacgacgcct ggcagctcgt ccgccacttc
     1261 gacgaccgaa aacaggaacg cttcctctgc tgcacgctga atggcgctca cgatgtaatc
     1321 gccgtccgcg tcataaccgt cggcctcgtc aacaggacca ttgtccatcc tcgcgagatc
     1381 tacgcggaag ccgtggccga ccgcgcctgc gccatcttcg tcgcccacaa ccacccgtcg
     1441 ggtcggctcg agccgtcgcc ggaggacctg gacatcacca cgcggctcaa agaagccggc
     1501 ttggtgctcg gtatacccct cctcgaccat ctcgtcttct cgcacgccgg ttatacgagc
     1561 atggtggaga gcgggctgct cgtgcctgac aaggcctgag aaccgtcgct acgcttcgtc
     1621 ctcggcctgc atacgggact tgccgaagat gccacggaac atatcgccga ccaggtttct
     1681 cctggcgacg acccgcgctc cggtaaagga ggtcacgtcc ttctcggagc cggcgcacag
     1741 gagcacgtcg ccttcgcgca tccgatactt gggatcgaag
//
LOCUS       DAOPHV010000575         1776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_95443_length_1776_cov_3.276583, whole genome shotgun sequence.
ACCESSION   DAOPHV010000575 DAOPHV010000000
VERSION     DAOPHV010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95443_length_1776_cov_3.276583"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1776
                     /gene="pnp"
                     /locus_tag="PLQ29_12255"
     CDS             <1..>1776
                     /gene="pnp"
                     /locus_tag="PLQ29_12255"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPG87460.1"
                     /translation="IQVVPTTVSADLVNPPDIIAINAASAAVHISDIPFNGPIAAVRV
                     CYVGGTYVINPTYDQIEKSALEIVVAGSEEGITMVEGGADEVSEELMIGAIEAAKKPI
                     ADICGVIRAMRDAVGKPKLALAPLKAELARKAEIEAFGKERIGKAIFTKVKHERYAAV
                     AKAIAETKATFADSLGDDEVQLKLFAALMEDIQYNLLRSGILDRGQRVDGRGTEDIRP
                     ITCEIGVLARAHGSALFTRGETQALATTTLGTTFDEQVLDDIEGDRRERFMLHYNFPP
                     FSVGEVGRLGTGRREIGHGHLARRALERVLPTKEQFPYTIRVVSDILESNGSSSMASV
                     CGGCLSLLAAGVPIKAPVAGIAMGLITEGDRYAVLSDILGDEDHLGDMDFKVAGTEKG
                     ITGFQMDIKIAKVSTEILTKALDQARRGRLHILGVMNQTIAAPAKEISEYAPKILSVK
                     IDVDKIGAIIGPGGKNIKALCEQYGVRINVDDDGTVTIFGANQKASLEAKAAVLGIAQ
                     DPEVGIVYQGTVKRVMDFGAFVEILPGKEGLVHISRLSRTRINAASDVVKEGQVIPVR
                     LLEVDKLGRLNLSYIDAMDDAGQKK"
BASE COUNT          348 a          597 c          544 g          287 t
ORIGIN      
        1 agatccaggt tgtgccgacg accgtctccg ccgacctcgt caaccccccc gacattattg
       61 ccatcaacgc ggccagcgcc gccgttcata tcagcgacat cccgttcaac ggacccatag
      121 ccgccgtgcg cgtctgctac gtcggcggca cctacgtcat caatccgacc tacgaccaga
      181 tcgagaagtc ggccctggag atcgtcgtcg ccggttccga ggaaggcatc acgatggttg
      241 agggcggcgc cgacgaggtg tccgaggaac tgatgatcgg ggctatcgag gccgccaaga
      301 aacctatcgc cgacatctgc ggcgtcatcc gcgccatgcg cgacgccgtc ggtaagccca
      361 agctggcgct cgcgccgctt aaggccgagc tcgcccgcaa ggccgagatc gaggccttcg
      421 gcaaggagcg catcggcaag gctattttca ccaaggtcaa gcatgagcgt tacgccgcgg
      481 tcgccaaggc catcgccgag accaaggcaa ctttcgccga ctcgctcggc gacgacgaag
      541 tccagctcaa gctgttcgcc gctctgatgg aggacatcca gtataaccta ctgcgctccg
      601 gtatcctcga ccgcggacag cgcgtcgacg gccgcggcac cgaggacata cgtccgatca
      661 cctgcgagat cggcgtgctg gccagggccc acggctccgc gctgttcacg cgcggcgaga
      721 cccaggccct cgcgacgact accctcggta cgaccttcga cgagcaggtg ctcgacgaca
      781 tcgagggcga ccggcgcgaa cgctttatgc tgcactataa cttccctccg ttctcggtcg
      841 gcgaggtagg gcgcctcggc accggccgcc gcgagatagg ccacggccac ctggccaggc
      901 gcgcgctcga gcgcgtgttg ccgaccaagg agcagttccc ctatacgatc cgcgtcgtct
      961 ccgacatcct cgagtcgaac ggctcgtcct cgatggcctc agtctgcggc ggttgtctat
     1021 ccctgctcgc ggccggcgtg ccgatcaagg cgcccgtggc gggcatcgcg atgggactga
     1081 tcaccgaggg ggatcgctac gcggtactct cggatatcct cggagacgag gaccacctgg
     1141 gcgatatgga cttcaaggtt gccggtaccg agaagggcat caccggcttc cagatggaca
     1201 tcaagatcgc caaggtgtcg accgagatcc tgaccaaggc cctcgaccag gctcgccgcg
     1261 gcagactgca tatcctgggc gtcatgaacc agaccatcgc cgcgcccgcc aaggagatat
     1321 ccgagtacgc gcctaagatt ctctccgtca agatcgacgt ggacaagatc ggcgccatca
     1381 tcggcccggg cggtaagaac atcaaggccc tttgcgagca gtacggcgtc aggatcaacg
     1441 tcgacgacga cggcaccgtg accatcttcg gcgccaacca gaaggcgtcg ctcgaggcca
     1501 aggcagccgt gctcggcatc gcccaggatc ccgaggtcgg cattgtctac cagggcaccg
     1561 tcaagcgcgt gatggacttc ggcgccttcg tagagatctt gcccggcaag gaaggcctcg
     1621 tccatatctc caggctgtcg cggaccagaa tcaacgccgc cagcgacgtc gtcaaggaag
     1681 ggcaggtcat accggtcagg ctgcttgagg tcgacaagct cggccgcctg aacttgtcgt
     1741 atatcgacgc catggacgac gccggccaga agaagt
//
LOCUS       DAOPHV010000576         1775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_95531_length_1775_cov_3.163953, whole genome shotgun sequence.
ACCESSION   DAOPHV010000576 DAOPHV010000000
VERSION     DAOPHV010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95531_length_1775_cov_3.163953"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1589
                     /locus_tag="PLQ29_12260"
     CDS             <1..1589
                     /locus_tag="PLQ29_12260"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG87461.1"
                     /translation="RVVKDASLCGLGQTASNPVLTTLKYFRREYESHIVEQRCEGGTC
                     EALFEALCENSCPLHMNIPGYLALLGDGRIEDAFELTLRDNPLPGSMGRICHFHCQMR
                     CRRDTLDEPVAQGEIHRYLADTMYKMGREKDVWAKLVKERKPPTGRSIAIVGSGPAGL
                     AAGFYLSRLGHEVTVFESKPKAGGILRYGIPTYRLPKDVLDKELELWKRLGVKFVYNT
                     MVGRDVGFDSLRSQYDALIVAIGAQADKRLGIPGEELDGVHAGYAWLEAFSMGKATRR
                     GRRALVIGGGNVAIDAARTLFRLDCDVTVAYRRAKQSMPANAVELAGAEEEGIRFEYM
                     LQPLEALGDGGRVKAVCFRVMRPGGMDSSGRPKPVETDRTVDIPCDDVYVAVGERVDS
                     SFVASDGVTTTSDGRIETDRLTGKASGSVWAIGDAVTGPATAAEAMGLGKRVAKAIDA
                     ELMGREAFGELFKDFGHSMAVPPEPVKTGMYRSRLVPPEERRGNFQEISQGYSGDQAL
                     LECARCLRCDIRADARSPWR"
BASE COUNT          321 a          594 c          603 g          257 t
ORIGIN      
        1 cccgcgtcgt caaggacgcc tcgctctgcg gcctcggcca gaccgcctcc aaccccgtgc
       61 tcaccacgct caagtacttc cggcgggaat acgagagcca tatcgtcgag cagcgctgcg
      121 agggcggtac ctgcgaggct ctgttcgagg cgctctgcga gaactcctgc ccgcttcata
      181 tgaacatacc cggctacctg gccctgctcg gcgacggccg catcgaggac gcgttcgagc
      241 tgaccctgcg cgacaacccg ctacccggct cgatggggcg catctgccac ttccactgcc
      301 agatgcgctg ccgccgcgac acgctcgacg agccggtggc ccagggcgag atccaccgct
      361 acctggccga cacgatgtac aagatggggc gggagaagga cgtctgggcc aagctcgtca
      421 aggagaggaa gccgcccacg ggacgctcga tagccatcgt cggttcgggc cccgcgggcc
      481 tcgccgccgg cttctacctg tccaggctcg gccacgaggt cacggtcttc gagtccaagc
      541 ccaaggccgg cggcatactg cgctacggca tcccgaccta ccggctaccc aaggacgtgc
      601 tcgacaagga actggagcta tggaagcggc tcggcgtcaa attcgtgtac aacacgatgg
      661 tgggccggga cgtgggcttc gattcgctac gcagccagta cgacgcgctg atagtcgcca
      721 taggcgccca ggcggataag cgcctcggga tacccggcga ggagctcgac ggcgtacacg
      781 ccggctatgc ctggctcgag gcgttctcga tgggcaaggc gacgaggcga ggccggcgcg
      841 ccctggtcat cggcggcggc aacgtcgcca tcgacgcggc caggactctt ttcaggctgg
      901 actgcgacgt taccgtggcc taccggcgcg ccaagcagag catgccggcc aacgcggtcg
      961 agctggccgg cgccgaggag gaaggcatcc gcttcgagta catgctgcag cccctcgagg
     1021 ccctcggcga cggagggcgc gtcaaggcgg tctgcttcag ggtaatgcgg ccgggaggca
     1081 tggatagtag cggccgaccc aagccggtcg agaccgaccg gaccgtggac ataccctgcg
     1141 acgacgtcta cgtggcggtc ggcgaacggg tggactcctc gttcgtcgcc tccgacggcg
     1201 ttacaaccac cagcgacgga cggatcgaga ccgacaggct gaccggcaag gcctcgggct
     1261 ccgtatgggc gataggcgac gccgtaaccg ggccggccac ggcggccgag gctatgggcc
     1321 tcggcaagcg ggtcgccaag gcgatcgacg ccgagctcat gggccgcgaa gcgttcggcg
     1381 agctattcaa ggacttcggc cactcgatgg ccgtaccgcc ggagccggtc aagacgggca
     1441 tgtaccgctc gaggctcgtg ccgccggagg aacgccgggg caatttccag gagatatccc
     1501 agggatactc cggagaccag gccctgctcg agtgcgcgcg ctgcctgcgc tgcgacatca
     1561 gggccgacgc ccgtagccca tggcgatgat cggaggatag catggatgga accaaagcca
     1621 agacccttag cctatcgatc gacggccgag agctgagcgt acgcgaaggg acgacgatac
     1681 tcgaggcctg ccgcggtgct ggcgtcagga taccgaccct ctgccacctg ccgggtgtca
     1741 gctcgaacgc ctcatgcggc ctctgcgtcg tcgag
//
LOCUS       DAOPHV010000577         1774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_95618_length_1774_cov_2.499709, whole genome shotgun sequence.
ACCESSION   DAOPHV010000577 DAOPHV010000000
VERSION     DAOPHV010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95618_length_1774_cov_2.499709"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PLQ29_12265"
     CDS             <1..694
                     /locus_tag="PLQ29_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HPG87462.1"
                     /translation="ACTYILSFLLLKALTWALGFAGNAGRELAVNLWGMNFIFSALTA
                     MLVRAIVDKLRIGYVLDDDSLSRISGIAVDYMVTGSLAAISLVFVGRYWLPLLLLSTF
                     GGVIVAITIPWLCSRIFRDHRYERMIMIFGVSTGTLSTGLALLRILDPEFKTKVSSDY
                     MLSAGMTFALMLPFVLAVNLPAQAGQSGSLGPFWTMIGIAAAYLLYSIVAYALVARSR
                     SLAKPHRFWLED"
     gene            complement(691..>1774)
                     /locus_tag="PLQ29_12270"
     CDS             complement(691..>1774)
                     /locus_tag="PLQ29_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87463.1"
                     /translation="VIVPTHQGTLDPFMVGYFVPEPVYWVTSDGNMRSSIMKLLLGMV
                     GSIPKSKAIPDMETIGWIMDVIRKRGGVVGIFAEGQASWNGHTQEIIPSTAKLLKLLK
                     VPVIAAVLKGAYFSQPRWSWNKRPGDMEIEFKLVMDGDEAKASTTERIHEALVEAMEF
                     DEEEWRADHPARHRGNGRARHLELALFMCPVCGKAGSMRSHVNRLYCRSCGHVVRLSR
                     SYAFTPVGECEPRFRTIREWDAWQASAFESALRGAASGEPIFSDGGVMLLRGYRMNPL
                     RRIRTGVMILYRDRIELATLLGERLAFPIADIEGAGVLKQQLFEFYIGKSLYQFRFPR
                     RFQSARKWHGAVSVLKRLASERSDGS"
BASE COUNT          296 a          594 c          531 g          353 t
ORIGIN      
        1 agcctgtacc tatatcctgt cgttcctgct gctcaaggcc ctgacctggg cgctcggctt
       61 cgccgggaac gccggtcgcg aactggccgt gaacctctgg ggcatgaact tcatcttctc
      121 cgccctgacc gccatgctcg tgcgagccat cgtcgataag ctacggattg gctacgtcct
      181 cgatgacgac agcctatcca ggatatccgg catagccgtc gactacatgg taaccgggtc
      241 gctcgcggcc atttcactgg tctttgtcgg ccggtactgg ctaccgctgc tactgctgtc
      301 caccttcggc ggcgtcatcg tcgcgatcac cataccgtgg ctgtgctcga ggatattcag
      361 ggaccatcgt tacgagcgga tgatcatgat cttcggcgtt tcgaccggta ccctgtcgac
      421 cggcctggcc ctgctgcgga tactcgaccc ggaattcaag accaaggtgt cgagcgacta
      481 catgctgtcc gccggcatga ccttcgccct gatgctaccg ttcgtgctgg ccgtcaacct
      541 gcccgcccag gccgggcagt cgggttcgct cgggccgttc tggacgatga tcggcatagc
      601 ggccgcctac ctgctgtact cgatcgtcgc gtacgcgctg gtcgcccgtt ccaggtcgct
      661 ggccaagccg cacaggttct ggctcgagga ctaggagccg tcgctgcgct cggacgctag
      721 gcgcttcagt acgctcacgg cgccgtgcca cttgcgggcg gactggaagc gcctcggaaa
      781 gcggaactga tacagcgact ttcctatata gaactcgaac agttgctgtt tgagcacgcc
      841 ggcgccctcg atatcggcga tagggaaggc gagccgctcg ccgagcaggg tcgccagctc
      901 gatacggtcg cgatagagta tcatcacgcc ggtcctgatc ctcctgagcg ggttcatgcg
      961 atacccacgt agcagcatca ccccgccgtc ggagaagata ggctcgcctg aggccgctcc
     1021 cctgagcgcc gactcgaagg cggacgcctg ccaggcgtcc cattcgcgta tggtcctgaa
     1081 cctgggctcg cattcgccta ccggagtgaa cgcatagcta cgcgaaagcc tgacgacgtg
     1141 gccgcatgac cggcagtaca gccggttgac gtggctcctc atcgagcccg ccttcccgca
     1201 gaccgggcac atgaatagcg ccagctccag gtgcctggcc ctgccgttcc cgcggtgccg
     1261 cgccggatgg tcggcccgcc attcctcctc gtcgaactcc atagcctcga ccagggcctc
     1321 gtggatgcgt tccgtggtgc tggccttggc ctcgtcgcca tccatcacca gcttgaactc
     1381 gatctccata tcgccgggcc gcttgttcca cgaccagcgc ggttggctga aataggcgcc
     1441 cttgagcacc gccgcgatca ccggcacctt gagcagcttg aggagcttgg cggttgacgg
     1501 gatgatctcc tgcgtatgcc cgttccagct ggcctggccc tcggcgaaga tgccgacgac
     1561 gccgccgcgc ttgcggatca cgtccatgat ccagcctatc gtctccatat cggggatggc
     1621 cttggacttc gggatggatc cgaccatgcc caggagcagc ttcatgatcg acgagcgcat
     1681 attgccgtcg ctcgttaccc aatagacggg ctccggcacg aaatagccga ccatgaacgg
     1741 gtcgagcgtg ccctgatggg tcggcacgat cacg
//
LOCUS       DAOPHV010000578         1772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_95734_length_1772_cov_3.519511, whole genome shotgun sequence.
ACCESSION   DAOPHV010000578 DAOPHV010000000
VERSION     DAOPHV010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95734_length_1772_cov_3.519511"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PLQ29_12275"
     CDS             complement(<1..384)
                     /locus_tag="PLQ29_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87464.1"
                     /translation="MNAIPFKDLTPDTYYSEPVFIDESYILLTPETPLTSSLINRLAE
                     WEIRELYTDGAPLGGDEEPATAPEEAEGEAEGRPVTSQTAGDKENLDRVSTFYDQFLK
                     YVDSVYTRYVTKSEISLKELSDKVKT"
     gene            496..1326
                     /locus_tag="PLQ29_12280"
     CDS             496..1326
                     /locus_tag="PLQ29_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87465.1"
                     /translation="MKFKTIFILFNAILLFSFAFIFLMPFFLLGFDYSMPFWTKNWPL
                     FAFFAAVLVGFNAFFGRNWRLFTLLEAERWDELGVLLEDRVFVKKRRDSRTVRLLVNT
                     SLLRGDAATIERLENTLRAEKPRALRRDAVIFGAARLLRNDAEASVRFLSEYADGKGV
                     ENAAWIKFHHAFALVLDKRAREATAPLEASVGARDPVLSLLSAYLLGSLCAVSAVGGE
                     RDRLKAVAEAERSRLAGRYAARAWAREVERAKSEVHIVILSKILDEASEWLYRAPASA
                     "
     gene            1453..>1772
                     /locus_tag="PLQ29_12285"
     CDS             1453..>1772
                     /locus_tag="PLQ29_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HPG87466.1"
                     /translation="MSVSRPRSIAALLIAASFCVMIAVSAAVADPAVASGSRSHTVQK
                     GETLYAIAKRYDVSVAALMSLNGIADAGRLFVGMQLAIPGGSDTEPRRLDYVVKKGDT
                     LYGIA"
BASE COUNT          278 a          565 c          569 g          360 t
ORIGIN      
        1 cgtcttgacc ttgtcggaca gctccttgag gcttatctcg ctcttggtca cgtagcgcgt
       61 atagacggag tcgacgtatt tcaggaactg gtcgtagaac gtcgagacgc ggtcgaggtt
      121 ctccttgtcg ccggccgtct gggacgtgac gggacggcct tcggcctcgc cttcggcctc
      181 ctcaggcgcg gtggccggtt cctcgtcgcc gcccagcggg gcgccgtcgg tgtacagctc
      241 gcggatttcc cattcggcga gccggttgat cagcgacgaa gtcagaggcg tctcaggcgt
      301 caggaggatg tagctttcgt ctatgaagac cggctcgctg tagtacgtat ccggcgtcag
      361 atccttgaac gggatggcgt tcatgcccat tgacccctct caggcgatat tctatgcttt
      421 gtccgcgttt cgcgccaccg gcgaacggcg cgcagagccg ctaggccctc tcttctatta
      481 ttggagacaa tccgcatgaa gtttaagaca atattcatac ttttcaacgc gatcctcctg
      541 ttctcgttcg cttttatatt cctgatgccg ttcttcctgc tcgggttcga ctattcgatg
      601 ccgttctgga cgaagaactg gccgctgttc gcgttcttcg cggcggtgct cgtcggcttc
      661 aacgcgttct tcgggcggaa ctggaggttg ttcaccttgc tcgaggcgga acgctgggac
      721 gagcttggcg tcttgctcga ggatcgtgtc ttcgtcaaga agcgccgcga tagccgaacg
      781 gttcggctcc tcgtcaatac gtcgctgctc aggggggacg cggcgacgat agagcggctc
      841 gagaacacgc tacgcgctga aaaaccccgg gcgctcaggc gcgacgcggt gatcttcgga
      901 gccgcccgcc tgctgcgcaa cgacgccgag gcgagcgtgc gcttcctatc cgagtacgcc
      961 gacggcaagg gcgtcgagaa cgcggcctgg atcaaattcc accacgcctt cgccctcgta
     1021 ctcgacaagc gggctcgcga ggcgaccgcg ccgctcgagg cctcggtggg cgcgcgagat
     1081 ccggtgctgt cgctcctgtc ggcctatctg ctcggttcgc tgtgcgccgt ctccgcggtc
     1141 ggcggggagc gcgatcggct caaggccgtc gccgaggccg aacgctcgag gctggccgga
     1201 cgctacgccg cccgcgcctg ggctcgcgag gtcgagcggg ccaagagcga ggtgcacatc
     1261 gtcatcctgt ccaagatcct cgacgaggcg agcgagtggc tctatagggc cccagcgtcc
     1321 gcctagccct atccggcgcg acgcctagcc ctatccggcg cgacgcttaa cccgggccgg
     1381 cgcgacgcct agcccgggca gacctgtaat ctcgctcatg agccgtgcgg gcctggccga
     1441 caattgaggt gtatgagcgt atcacgaccg agatccatcg ccgcgctact gatagccgcc
     1501 tcgttctgcg tcatgatagc cgtgtcggcc gccgtggccg atccggccgt cgcgtccggt
     1561 tctcggtcgc atacggtaca gaagggcgag acgctgtacg cgatagccaa gcgctacgac
     1621 gtatcggtcg cggctctgat gagcctcaac ggcatcgcgg atgccggcag gttgttcgtc
     1681 ggcatgcagc tggccattcc gggcggttcg gacacggaac ctcgtcggct cgactacgtc
     1741 gtcaagaagg gcgacacgct ctacggcatc gc
//
LOCUS       DAOPHV010000579         1763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_96456_length_1763_cov_4.804450, whole genome shotgun sequence.
ACCESSION   DAOPHV010000579 DAOPHV010000000
VERSION     DAOPHV010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96456_length_1763_cov_4.804450"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1575)
                     /locus_tag="PLQ29_12290"
     CDS             complement(<1..1575)
                     /locus_tag="PLQ29_12290"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPG87467.1"
                     /translation="MVEILLVPSVRRGNGSGHLVRCFSLAAALGPKAAVYLADEPGPG
                     SWSADELRLAYPRETASTRIIGAIRSDARFRLVVLDRRRTNQDELERWIGIGAVAAID
                     EGGPARGSAHYLVDILPRIAGATRGRGVDAANLASLGFLALPVARREPPTRAGRVLVS
                     FGGEDPAGLADAFLSAALSSGLVTPQAVSVVSGALSGAAAEYEGVTALGPVQNLKEKL
                     RSYDLVVTQFGLTAFEAAWAGCAVLLLNPSDEHERLGRAVGFASLGVGRPNPRRLSAA
                     FSDIGGLAAASAAAAPPERLDLAAKLAELRPHHAGECPVCGRRLGEAIYRSERKTYLR
                     CPDCGLVRMTHFFPRDNPYAERAYFFDEYKAQYGRTYIDDIPSIRTACSKRLAVIESM
                     LPAGHEGQGVLDVGCAYGAFVAEAQARGWNAVGSDLAPDAVEYVKSTWKIPAFVGDFA
                     APAADGLYPRNLACLSMWYVIEHFDELGRVLRRVASLLRPGGVFAFSTPSGEGISAKR
                     DARRFYERSPDDHYTVW"
     gene            complement(1581..>1763)
                     /locus_tag="PLQ29_12295"
     CDS             complement(1581..>1763)
                     /locus_tag="PLQ29_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HPG87468.1"
                     /translation="PERFKSIRPECPPSYRLPEARVTVDTAEDYERAVAIVRELGNEP
                     SDEAVIAWLLSRREGR"
BASE COUNT          268 a          615 c          625 g          255 t
ORIGIN      
        1 ccagacggtg tagtggtcgt ccggcgatcg ctcgtagaat cggcgggcgt cgcgcttggc
       61 cgagatcccc tcgccggacg gcgtcgagaa ggcgaacacg ccgcctggcc gcaggagcga
      121 ggctacccgc cggagcaccc ggcccagctc gtcgaagtgc tcgatgacgt accacatgga
      181 caggcaggcg aggttccggg gatagaggcc gtcggctgcc ggggcggcga agtcgccgac
      241 gaaggcaggg atcttccagg tgctcttgac gtactcgacg gcgtcgggcg ccaggtccga
      301 gccgaccgcg ttccagcccc tcgcctgggc ctcggccacg aaggcgccat aggcgcagcc
      361 cacgtcgagt acgccctgcc cctcgtggcc ggccggtagc atggactcga tcaccgccag
      421 acgcttggaa caggccgtac ggatgctcgg gatatcgtcg atataggtgc ggccgtactg
      481 ggccttgtat tcatcgaaga aataggcccg ttcggcgtac gggttatccc gcgggaagaa
      541 atgcgtcatc ctgaccagcc cgcagtccgg gcagcgcagg taggtcttgc gctccgagcg
      601 atagatcgcc tcgccgaggc gccggccgca gacggggcat tcccccgcgt gatggggcct
      661 cagctcggcg agcttcgcgg ccaggtcgag ccgctccgga ggcgccgccg cggccgacgc
      721 cgccgcgagg ccgccgatgt cggagaaagc ggcgctcagg cggcgtgggt tcgggcggcc
      781 gacgccgagg ctcgcgaacc cgacggccct gccgagccgt tcgtgttcgt cgctcgggtt
      841 gaggagcagc accgcgcagc cggcccaggc cgcctcgaat gcggtcaggc cgaactgcgt
      901 aacgacaagg tcgtaggacc ggagcttctc cttgagattc tggaccgggc cgagggcggt
      961 cacgccctcg tactccgccg cggctccgga cagggcgccg gaaacgacgc tgactgcctg
     1021 gggcgttacc agcccgctgc tcagggccgc cgagaggaac gcgtccgcca atccggccgg
     1081 atcctctcct ccgaagctga cgagcacgcg gccggcgcgg gtcggcggct ctctgcgcgc
     1141 cacgggcagg gccaggaagc cgagcgacgc caggttggcc gcgtcgacgc cccgccctct
     1201 cgtggccccg gctatcctcg gcaggatgtc gacgaggtag tgggccgacc cgcgcgccgg
     1261 gccgccctcg tcgatagcgg cgacggcccc gatgcctatc catcgctcca gctcgtcctg
     1321 gttcgtacgg cgccggtcca gcacgacgag cctgaagcgc gcgtcggagc ggatggcgcc
     1381 gatgatcctc gtggacgcgg tctcgcgcgg ataggccagc ctgagctcgt cggcgctcca
     1441 cgagcccggg ccgggctcgt cggccaggta gacggctgcc ttcggtccca gtgccgccgc
     1501 cagcgagaaa cagcgcacca ggtggccgga accgttgcct cggcgaaccg acggtacgag
     1561 gagtatctct accatggcga ctacctaccc tctcggcgcg agagaagcca cgctataacg
     1621 gcttcgtcgc tcggttcgtt ccctagctcg cggactatgg cgacggcccg ctcgtagtcc
     1681 tcggccgtgt cgaccgtgac ccgcgcctct ggcaggcggt acgaaggcgg acattccggc
     1741 ctgatcgact tgaagcgctc ggg
//
LOCUS       DAOPHV010000580         1753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_97259_length_1753_cov_5.480565, whole genome shotgun sequence.
ACCESSION   DAOPHV010000580 DAOPHV010000000
VERSION     DAOPHV010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97259_length_1753_cov_5.480565"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PLQ29_12300"
     CDS             complement(<1..484)
                     /locus_tag="PLQ29_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87469.1"
                     /translation="MEKILLNPKQIVATYADEETRQIMLKTIDFFEGRGLKKMKDDFH
                     ACKWYTEFLDFMKENQIMAKLLTPSGFGGPASRWDTSRIVDFAEILGFYGLTYWYTFQ
                     VSELGLGPIFLGSNTEAKHKAANFLKEGAIFAFGLSEKEHGADIYSSDMMLYPNPDGT
                     Y"
     gene            complement(533..>1753)
                     /locus_tag="PLQ29_12305"
     CDS             complement(533..>1753)
                     /locus_tag="PLQ29_12305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPG87470.1"
                     /translation="GDKYYIGNGNEAAMVSTFAKMADSGDYVFFVADSKHEKYECVRN
                     VVNEQNYVAEYALHDYPITNADITEKGQKAWDDMLNTINVCKFNLGWGAIGLCTHALY
                     ESIDHAANRNVYGKFVTDFPHVKRLLTDAYCRLVAMKLFAERASDYMRSAGPEDRRYL
                     LYNPMVKMKVTREGEDVMNILHDVIAAKGFENEPFFEIAKHELPMLPKLEGTVHVNMA
                     LIVKFMANYFFNPKAYAEVPRRDDTANDEFMFHQGPTKGLGKIQFHDYNIAYNSVDLP
                     NVNIFKEQINLFKDFLMKAGPDKAQSSDIDYLLALGEVLTLVAYGQLIIESRKFIPVE
                     DDLLEEIFDFMVRDFSKYALTIHMKPSNSDKQKELSLKIIKTPLPNAERFEKIYKDHV
                     YALKGTYKMRDQNF"
BASE COUNT          322 a          462 c          523 g          446 t
ORIGIN      
        1 ggtaggtgcc gtccgggttg gggtacagca tcatgtcgct ggagtagata tcggccccat
       61 gctctttctc ggacaggccg aaagcgaaga tcgcgccttc tttaagaaaa ttggcggcct
      121 tgtgcttggc ctcggtgttg gaaccaagga agatgggtcc cagccccagt tcgcttacct
      181 ggaaggtgta ccagtaggtg aggccgtaga agcccaggat ttcggcaaag tcgacgatcc
      241 gggaggtgtc ccaccgcgat gcgggcccgc caaagccgct cggcgtaagt agcttcgcca
      301 tgatctggtt ttccttcatg aaatcgagga actcggtgta ccatttgcag gcgtggaaat
      361 catccttcat cttcttgagg ccccggccct cgaagaagtc gatggtcttg agcatgatct
      421 gcctggtttc ctcgtctgcg tatgtggcga cgatctgttt cgggtttaat aagatttttt
      481 ccatgattgt atcctttttt ttgtgccgcc gtgaatgcgg caaaattacg cattaaaaat
      541 tctggtccct catcttgtac gtgcctttaa gcgcgtagac gtggtccttg tatatcttct
      601 cgaaccgctc tgcgttcggc agcggtgtct tgattatctt tagcgaaagc tctttctgct
      661 tatcgctgtt cgacggcttc atgtggatgg taagcgcgta tttcgagaag tcgcgcacca
      721 tgaagtcgaa tatttcctcc aggaggtcgt cctcgaccgg aatgaacttc cggctctcta
      781 tgatgagctg gccgtaggcc acgagggtga ggacctcgcc gagcgccagg aggtagtcga
      841 tgtcgctcga ctgggccttg tccgggccgg ccttcatcag gaagtccttg aacaggttga
      901 tctgttcctt gaaaatgttc acgttgggca ggtccacgct gttataggct atgttgtaat
      961 cgtgaaactg gattttgccc agccccttgg tcgggccctg gtgaaacatg aactcgtcgt
     1021 tcgccgtatc gtcgcggcgc gggacctccg cataggcttt cgggttgaag aaatagttgg
     1081 ccatgaactt gacgatcagg gccatgttca cgtggacggt cccctcgagc ttcgggagca
     1141 tgggcagctc gtgcttggca atttcgaaga agggctcgtt ctcgaagccc ttggccgcga
     1201 tcacgtcgtg gaggatattc atcacgtcct cgccctcgcg cgtgactttc atcttgacca
     1261 tggggttata gaggaggtag cggcggtcct ccgggccggc cgagcgcatg tagtcgctgg
     1321 cgcgctcggc gaaaagcttc atcgccacca ggcggcagta ggcgtcggtc aggaggcgct
     1381 tcacgtgcgg gaagtccgta acgaacttgc cgtacacgtt ccggttggcg gcatggtcga
     1441 tcgactcgta cagcgcatgg gtacacagcc cgatggcgcc ccagcccagg ttgaacttgc
     1501 agacgttaat ggtgttgagc atgtcgtccc aggctttctg ccctttttcg gttatatcgg
     1561 cattggtgat cgggtagtcg tgcagcgcgt actcggcaac gtagttctgc tcgttcacca
     1621 cgttgcgcac gcactcatac ttctcgtgct tggagtcagc aacgaagaag acgtagtccc
     1681 ctgagtcggc catcttggcg aaggtggaga ccatggcggc ctcgttgccg ttgccgatgt
     1741 agtacttgtc gcc
//
LOCUS       DAOPHV010000581         1750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_97508_length_1750_cov_4.440118, whole genome shotgun sequence.
ACCESSION   DAOPHV010000581 DAOPHV010000000
VERSION     DAOPHV010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97508_length_1750_cov_4.440118"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PLQ29_12310"
     CDS             <1..356
                     /locus_tag="PLQ29_12310"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HPG87471.1"
                     /translation="SGRSHAENAATDPLLHLEMLKQWASYYAGGASLADPLVSPVFAD
                     FSGFPPLLLQVDSGELLLDDSLAIAERARAAGVDARLIRREGLWHVWPALGDLIPESR
                     QAFGAMKAFLASLGG"
     gene            409..1170
                     /locus_tag="PLQ29_12315"
     CDS             409..1170
                     /locus_tag="PLQ29_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPG87472.1"
                     /translation="MNIADFDDWAQELLGIQRFKPIDDSLNGLQVGRSDGPLERIAFA
                     VDASAETIRRARDAGAQALFVHHGLFWGKPAPLVGSMRARVAELLSADLALYACHLPL
                     DAHPELGNNAVLARLLGIVDPQPFGEYHGVKLGFEGRLDRPASVDEVVRRVLPYGDEP
                     RAMLRFGPASVSTVAVVSGGAPFEVLQAIAEGLDLYVTGEPSHSVYHEAMEAGINFAA
                     AGHYATEVHGVKAVAERVARDLGIRTLFIEYPTGL"
     gene            1204..1740
                     /gene="lspA"
                     /locus_tag="PLQ29_12320"
     CDS             1204..1740
                     /gene="lspA"
                     /locus_tag="PLQ29_12320"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPG87473.1"
                     /translation="MPEQKLRTPLAPFILTGGILALDQITKFAVVAAVKPGRIAWRAF
                     GDFFWLVHQKNTGAAFSMGDGLPEPLRVAILIVLPLALMAALCVYYFKTDELTKVQRW
                     ALCGIVGGGVGNIIDRIARPDGVIDFLSVKFYGLFGLERWPTFNVADSAVVVCGILLV
                     VSGFFVAPKGDESERPTP"
BASE COUNT          262 a          598 c          576 g          314 t
ORIGIN      
        1 actccggccg tagccacgcc gagaacgccg ccacggatcc cctcctgcac ctcgagatgc
       61 tcaagcaatg ggcgtcgtat tacgccggcg gggcttctct ggccgatccc ctggtctctc
      121 cggtcttcgc agatttctcc ggcttcccgc ctttgctcct gcaggtagat tccggggagc
      181 tgctgctcga cgactcgttg gcgatagccg agcgggcgag ggcggccggc gtggacgcga
      241 ggctgatacg ccgcgagggg ctgtggcatg tctggccggc gctcggcgac ctgatacccg
      301 agagccggca ggctttcggt gctatgaagg cgtttctcgc gtcgttgggc gggtgacgat
      361 cgaccccagc cttgtccgcg ctcggccgtc cggggtatac tgatccccat gaatatcgcc
      421 gatttcgacg actgggccca ggagctcctg ggcatacagc gcttcaaacc catcgacgac
      481 tcgctcaacg ggctccaggt cggccgatcc gacgggccgc tcgagcggat agccttcgcg
      541 gtcgacgcca gtgccgagac catacgccgc gccagggacg cgggcgcgca ggccctgttc
      601 gtccaccacg gtctcttctg gggcaagccg gcgccgctgg tgggctcgat gcgcgcgcgc
      661 gtcgccgagc tcctgtccgc cgacctggcg ctgtacgcct gccacctgcc gctcgacgcg
      721 catcccgagc tcggtaacaa cgccgtgctg gccaggctcc tcggcatcgt cgacccgcag
      781 cccttcggcg agtaccacgg cgtcaagctc ggcttcgagg gccggctcga taggccggca
      841 tccgtcgacg aggtcgtcag gcgcgtcttg ccgtacggag acgagcccag ggcgatgctc
      901 cgcttcggcc cggcctcggt gtcgacggta gccgtcgtgt ccggaggcgc gccgttcgag
      961 gttctgcagg ccatcgccga gggccttgac ctctacgtga ccggggagcc gtctcattcc
     1021 gtctaccacg aggcgatgga ggccggcatc aatttcgcgg cggccggcca ctacgccacc
     1081 gaggtgcacg gcgtcaaggc cgtcgccgag cgggtcgccc gcgacctcgg catacggacg
     1141 ctgttcatcg agtacccgac cgggctgtaa agcgccggaa tactatcgaa ggctggagcc
     1201 atcatgccag agcagaagct acgtacaccg ctcgccccgt tcatcctgac gggcgggatc
     1261 ctggcgctcg accagataac caagttcgcc gtcgtcgcgg ccgtcaagcc cgggcgtata
     1321 gcctggagag ccttcgggga cttcttctgg ctcgtgcacc agaagaacac cggcgcggcc
     1381 ttcagcatgg gcgacggcct acccgagccg ttgcgcgtgg ccatcctgat cgtgctgccg
     1441 ctcgccctga tggcggccct ctgcgtctac tatttcaaga ccgacgagct gacgaaggtc
     1501 cagcgctggg ccctctgcgg catagtcgga ggcggggtcg gtaatatcat cgaccgtata
     1561 gccaggcccg atggcgtcat tgacttccta tcggttaaat tctacgggct gttcggcctg
     1621 gagcgctggc cgaccttcaa cgtggccgat tcggccgtcg tcgtatgcgg catcctgctg
     1681 gtcgtcagcg gtttcttcgt agcgcccaag ggcgacgaat cggagcggcc gacgccttga
     1741 gccgacgcga
//
LOCUS       DAOPHV010000582         1750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_97518_length_1750_cov_3.568142, whole genome shotgun sequence.
ACCESSION   DAOPHV010000582 DAOPHV010000000
VERSION     DAOPHV010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97518_length_1750_cov_3.568142"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLQ29_12325"
     CDS             complement(<1..283)
                     /locus_tag="PLQ29_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HPG87474.1"
                     /translation="MYVVLITLGFSALLAFVLGAALGFFKEKFKVERDPKIDQVRAAL
                     PGVNCGGCGYPGCDGYAEAVAKGDAPITKCAPGGKAVADALGTLMGVSAS"
     gene            complement(297..872)
                     /locus_tag="PLQ29_12330"
     CDS             complement(297..872)
                     /locus_tag="PLQ29_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HPG87475.1"
                     /translation="MNLAKIFLSALLIDNIVLMRFLALCPFIGMSIDVSKSVGMGLAV
                     TFVTVIATVVTWPLYNFVLLPLNLTFLKILVFILVIASLVQLVEFFLKKNVPGLYASM
                     GIYLPLITTNCAILAVTFDNVSKGFNLIESVVYAVGVALGFMLSLVLLAGVRDRLKTA
                     PIPKFLQGTPILFASTSLLAVAFMGFSGLIK"
     gene            complement(869..1492)
                     /gene="rsxE"
                     /locus_tag="PLQ29_12335"
     CDS             complement(869..1492)
                     /gene="rsxE"
                     /locus_tag="PLQ29_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HPG87476.1"
                     /translation="MKRMWKIFVKGLVVENPLLMLMIGLCSSVAVTSSVSNGIGMGLA
                     MTFVIVFAELVISLFRKLIPNDARIPIFIIVIAAFTTMVDLSMQAYFPALSKSMGVFI
                     PLIVVNCIIMGRVEAFASKEKPLDAVVDALGMGLGYTWVLIGIAALRELLGNGTLLGA
                     RIMPEAFQPILFFTLPPGGFMVFALFISLNLWLKKKITNGAAVREAA"
     gene            complement(1489..>1750)
                     /locus_tag="PLQ29_12340"
     CDS             complement(1489..>1750)
                     /locus_tag="PLQ29_12340"
                     /inference="COORDINATES: protein motif:HMM:NF023149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HPG87477.1"
                     /translation="PAMASDSMPYETEWFSKEIRARIIDLPDGDCTVRLGFQEVILDS
                     VGERTMDIMINGVCVAEDFDIVKEAGAVGSAWLEANASGGSR"
BASE COUNT          390 a          510 c          536 g          314 t
ORIGIN      
        1 ccgaggcgct gactcccatg agcgtaccga gcgcatcggc gacggccttt ccgccaggag
       61 cgcacttagt aataggcgcg tcgcccttgg caaccgcttc tgcgtagccg tcgcagcccg
      121 gatacccgca cccgccgcag ttgacgcccg gcagggcggc tcggacctgg tcgatcttgg
      181 ggtcgcgctc gaccttgaac ttttccttga agaacccgag ggcggcgccg agcacgaagg
      241 cgagaagcgc ggagaatccg agcgttatca atacgacata catttccgga tactccttac
      301 ttgatcaggc cggagaagcc cataaacgcg acggcgagca ggctcgtcga ggcgaataga
      361 atgggcgtac cctgcaggaa cttaggtatc ggagccgtct taaggcgatc ccgaaccccg
      421 gccagcaata cgagcgacag catgaacccg agcgctacgc ccacggcgta cacgaccgac
      481 tcgatcaggt tgaagccctt ggatacgttg tcgaaggtga cggccaggat agcgcagttc
      541 gtcgtgatca gcggcaggta gatgcccatg gaagcgtaga ggcccggtac gttcttcttg
      601 aggaagaatt ccacgagctg tacgagcgat gcgatgacca ggatgaacac gagtatcttg
      661 aggaacgtca ggttaagcgg cagcaggacg aaattgtaga gcggccaggt tacgacggtc
      721 gcaattaccg tcacgaacgt gactgcgagg cccatgccga cgctcttcga gacgtcgatc
      781 gacatgccga tgaagggaca gagggcgagg aaacgcatca gcacgatgtt gtcgatcagc
      841 aaggctgaaa ggaatatctt cgcgaggttc atgctgcctc ccgtacggcg gcgccgttcg
      901 ttatcttctt cttgagccag agattaagcg agatgaacag ggcgaacacc ataaagccgc
      961 ctggcggcag ggtaaagaaa aggatgggct ggaacgcctc gggcatgatt cgcgcgccga
     1021 gtagcgtacc gttgccgagc agctcgcgga gagcggctat cccgatgagt acccacgtat
     1081 agccgaggcc catgccgagc gcgtcgacga cggcgtcgag cggcttctcc ttgctcgcga
     1141 aggcctcgac ccggcccata atgatgcagt tcaccacgat cagcggtata aagacgccca
     1201 tcgacttcga aagcgccggg aagtatgcct gcatcgatag gtcgaccatg gtcgtgaacg
     1261 cggcgatgac gatgatgaag atcggaatcc gcgcgtcgtt cgggatcagc ttcctgaaca
     1321 gggagatgac tagctccgcg aatacgatca cgaaggtcat cgcgaggccc atgccgatac
     1381 cgttcgacac gcttgaggtg acggcgaccg acgaacagag gccgatcatc agcatgagga
     1441 gcgggttctc gacgaccagc cccttcacga atattttcca catgcgcttc atcggctgcc
     1501 tccgctggcg tttgcctcga gccaggcgga tcctacggcg cccgcctcct tgacgatatc
     1561 gaagtcctcg gccacgcaca cgccattgat catgatgtcc attgttcgtt cgcccacgga
     1621 gtcgagaatg acctcctgga atcccagccg tacggtgcag tcgccgtcag gaaggtcgat
     1681 gatgcgcgcg cggatttcct tgctgaacca ctctgtctca tagggcatgg agtcagaggc
     1741 catcgccggt
//
LOCUS       DAOPHV010000583         1748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_97725_length_1748_cov_2.621973, whole genome shotgun sequence.
ACCESSION   DAOPHV010000583 DAOPHV010000000
VERSION     DAOPHV010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97725_length_1748_cov_2.621973"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            696..>1748
                     /locus_tag="PLQ29_12345"
     CDS             696..>1748
                     /locus_tag="PLQ29_12345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HPG87478.1"
                     /translation="MRRESASSSSSSSRIAPEKGGRIVAPGPIARTDDEAASVGLVDR
                     YRDRLRATHYSPMTERAYLYWLERYLKGRPLPKPSDRPEASINAFVTKLAVQENVSAS
                     TQNQALAAILFLYRQVLAVEVGDLGELIRAKRPIRMPVVLSPEEVRSVLTVMSGEMRL
                     MASLLYGTGMRLNECLSLRVQDIDFNRNTVIIRDGKGGKDRGTILPSSLIKPLQDHLK
                     RVRAIHEADLKDGWGKAQLPTSLEKKYPNAASEWIWQWVFPQKRRWRDSKSKSEGRFH
                     MDASILQRAVREAMIIAGITKHASCHTFRHSFATQLLENGYDIRTVQELLGHSDIRTT
                     MVYTHVLNKGPGGVRSP"
BASE COUNT          388 a          436 c          512 g          412 t
ORIGIN      
        1 ctttatccag atcgatatcg atggcgtcgt ctaatcctat ggtgggggtt tcggtaatcc
       61 agactgcgct attagcttat gggtcgccga accagcgccg ggagaggcct cttggtcgag
      121 ccatgtgtcg gtctccgggt tcgaggtttt ccttgcgctt gtcgatgttt ttatggacaa
      181 atagggctgg tgaggctaga ctgctttata taggttcggg gtagcctgtt tcggcggcat
      241 tccggatgaa aattattggt acataattac aacgtaggcg acggctctga tgtgccaatc
      301 tcctcctctt gcccaactgg gctatccata gtgtctacgt atgcacataa aatgccgctt
      361 tcatttcaat taatgaaacc gttgatgggc cgaagagtaa acccttgtaa agggtggagg
      421 atagggccat gactgtcacg attaaatggg ttggggattg ttggctatcg gtcgccttct
      481 gtcctgctga aagcgggtct ggcattgttc acgctatccc aggatttggt ttcgatagcc
      541 tgcgcaacgc gtggatcgtt ccgggaaccc aggaaggggt ggataggcta ctcgctgggc
      601 tgtggtctag tcgggctttc ggctatatcg agccgcgcgg tgagtgtcgg gtggcggcgc
      661 tcgtgccggc tcctagcctg ctcgtaggaa acgcaatgcg acgcgagtcg gccagttcgt
      721 cgagttcatc gagtcgaata gcgccggaaa aagggggccg catcgtagcc cccgggccga
      781 ttgcgcgtac cgatgatgaa gcggctagcg tcggcttagt cgatcggtac cgcgatcggt
      841 tgcgggcaac tcattatagc ccgatgactg agcgagccta tctctactgg ctcgagcgtt
      901 acttgaaggg gcgtccccta ccaaagccta gtgatcgtcc cgaggcgtca ataaacgcct
      961 tcgtgacgaa gctagcggta caagagaacg tcagtgcctc gactcagaac caagccctag
     1021 ccgctatact gttcctgtac cgacaggtac tcgctgtcga ggtgggagac ttgggagaac
     1081 tgatacgagc aaaaaggccg atcaggatgc cggttgtctt aagccctgaa gaggtgcgat
     1141 cggttcttac agtaatgagc ggcgagatgc ggctcatggc ctccttgctc tacggaacgg
     1201 gcatgcgctt aaacgaatgt ctttcgctca gggtacagga tatcgatttt aataggaaca
     1261 ccgttatcat ccgcgatggc aaaggaggca aggatagagg cactattctg ccttcgtccc
     1321 tcatcaaacc gcttcaggat catcttaagc gcgtcagagc tattcacgag gccgacttga
     1381 aagacggttg gggcaaggcc cagttaccga cgtcactcga gaagaagtat cctaacgcgg
     1441 cttccgaatg gatatggcag tgggtattcc cgcagaaacg acgatggcgc gactcgaaat
     1501 ctaagtcgga agggcgattc catatggacg cctccattct gcaacgagct gtacgtgaag
     1561 ctatgataat cgcaggcatc acgaaacatg ccagttgcca tacattcagg cattctttcg
     1621 ccacgcagct acttgagaac ggctatgaca tcaggacggt tcaggagctc cttggccata
     1681 gcgacatccg tacaacgatg gtctacacgc atgttctcaa caaagggccg ggcggggttc
     1741 gtagtcct
//
LOCUS       DAOPHV010000584         1745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_97974_length_1745_cov_3.002367, whole genome shotgun sequence.
ACCESSION   DAOPHV010000584 DAOPHV010000000
VERSION     DAOPHV010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97974_length_1745_cov_3.002367"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..900
                     /locus_tag="PLQ29_12350"
     CDS             37..900
                     /locus_tag="PLQ29_12350"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPG87479.1"
                     /translation="MALTTEERRRLEDKAHELRLLSVDTIAWAGSGHVGGSMSAMDIM
                     TILYHKYMRVDPKDPAKPDRDRFILSKGHVGVGYAPLLADLGYFDKELLKEYNHTGSP
                     LGMHLDANKVRGVEASTGSLGHGLAIGLGLALGARLKGEGWKTFVLLGDGECDEGEVW
                     ESAMAAAHFKATNLVVIVDRNHCMIDGPTEDVMALEPFADKWRAFGFEVIEADGHDFD
                     ALASALDRATAATDKPVVIIADTIKGCGIDFIAGDYKWHYGGFDADRLGQAKASLERY
                     HESRLTKGAER"
     gene            897..>1745
                     /locus_tag="PLQ29_12355"
     CDS             897..>1745
                     /locus_tag="PLQ29_12355"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPG87480.1"
                     /translation="MSGLTFTMLDTAKMSTAEIYGQALCELGEEHPEIVGLSADLAKS
                     TKIGAFSDKFPDRFFNVGIAEQNMFGMAAGLAKASFVPYVSTFAIFTAMRGLDQVHTD
                     ICYQNLDVKMIATHAGLSFGQAGTTHHCTEDIAIMRSMANLTVVVPADGIETAHAVRA
                     AYDRKGPVYIRINRGFDQVVYDQPDYGFELGKAVTMRDGTDLTVIACGSTVWRAVEAA
                     AMLEKSDGLKVRVLNMHTIKPIDSEAIERAVRETRRVITVEDHSVVGGLGSAVAEVAA
                     GTGKSFS"
BASE COUNT          340 a          570 c          575 g          260 t
ORIGIN      
        1 gtcgacccgc gcgcggccgg aaaagggagg tagacaatgg cattaacgac cgaggagagg
       61 cgacgcctcg aggacaaggc ccatgagctg cggctcctga gcgtcgacac gatcgcctgg
      121 gcgggctcgg gccacgtcgg cggctcgatg tcggcgatgg acatcatgac catcctgtac
      181 cacaagtaca tgcgcgtcga ccccaaggat ccggccaagc cggaccggga ccgcttcatc
      241 ctgtccaagg gccacgtcgg cgtcggctac gcgccgctcc tggccgacct cggctacttc
      301 gacaaggaac tgctcaagga atacaaccat accggcagcc cgctcggcat gcacctcgac
      361 gcgaacaagg tgcgcggcgt cgaggcgtcg accggctcgc tcggccacgg cctggccata
      421 ggcctcggcc tggcgctcgg cgccaggctc aagggcgagg gctggaagac ctttgtgctc
      481 ctcggcgacg gcgagtgcga cgaaggcgag gtctgggaat cggcgatggc ggcggcccac
      541 ttcaaggcga cgaacctggt cgtcatcgtc gaccgcaacc actgcatgat agacggcccg
      601 accgaggacg tgatggccct cgagccgttc gccgacaagt ggcgggcctt cggcttcgag
      661 gtcatcgagg cggacggcca cgacttcgac gcgctggcct ctgcgctcga ccgcgctacc
      721 gccgcgaccg acaagcccgt ggtcatcata gccgacacga tcaagggttg cggcatcgac
      781 ttcatagccg gcgactacaa gtggcactac ggcggattcg acgctgacag gctcggccag
      841 gccaaggcca gcctcgagcg ctaccacgag tccaggctga cgaaaggagc cgaacgatga
      901 gcggcttgac ttttacgatg ctcgacacgg ccaagatgtc gacggctgag atatacgggc
      961 aggcgctatg cgagctgggc gaggagcacc ccgagatagt cgggctgtcc gccgacctgg
     1021 ccaaatcgac caagataggc gccttcagcg acaaattccc cgaccgcttc tttaacgtcg
     1081 gcatagccga gcagaatatg ttcggcatgg ctgcgggcct ggccaaggcc agcttcgtgc
     1141 cctacgtgtc gaccttcgcg atctttaccg ccatgcgcgg cctcgaccag gtgcataccg
     1201 acatctgcta ccagaacctc gacgtcaaga tgatagcgac ccacgccggg ctgtcgttcg
     1261 gccaggcggg cacgacgcac cattgcaccg aggacatcgc catcatgcgc tcgatggcca
     1321 acctgaccgt cgtcgttccg gcggacggca tcgagacggc gcacgcggta cgcgcggcct
     1381 acgatcgcaa gggcccggtg tacatacgca tcaaccgcgg cttcgaccag gttgtctatg
     1441 accagccgga ctacggcttc gagctcggca aggccgtgac gatgcgcgac ggtaccgacc
     1501 tgaccgtcat agcctgcggc tcgaccgtct ggcgcgccgt cgaggcggcc gcgatgctcg
     1561 agaagtccga cgggctcaag gtacgcgtgc tgaacatgca tacgatcaag ccgatagact
     1621 ccgaggcgat cgagcgggcg gttagggaga cgaggcgcgt catcaccgtc gaggaccaca
     1681 gcgtcgtagg cgggctcggc agcgccgtag cggaggtcgc ggcgggcacg ggcaagtcgt
     1741 tcagc
//
LOCUS       DAOPHV010000585         1743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_98186_length_1743_cov_2.060427, whole genome shotgun sequence.
ACCESSION   DAOPHV010000585 DAOPHV010000000
VERSION     DAOPHV010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98186_length_1743_cov_2.060427"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="PLQ29_12360"
     CDS             <1..1262
                     /locus_tag="PLQ29_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPG87481.1"
                     /translation="RYVNPSYTRITAVEESDIVGKPLAEARPGSHLPTVVATGVQELG
                     VRRKQGNAEYMVNMVPICRAGVIIGGISILNEVNDVYRLVKELDRSNRLIQRLKDRVR
                     QIGNARYSFDDIVSNDARSQATKDLAARLARKDVNLLILGESGTGKELYAQSIHNASQ
                     RSNHPFLAVNCAALDPNLLESELFGYEDGSFTGARKGGKIGLFEEASGGTLFLDEIGE
                     LDVRLQAKLLRALQEGLIRPVGGASEVSVDVRVMAATNKDIETLAKDGSFRRDLYYRV
                     ATFTLILSPLRERRGDITPLVQKFTNDIAGRYRATLDIDPDAMAALEAWDWPGNVREL
                     KNTIEYAAIMAEDGRISARDLPPRMVHGRAGTAPDEVRPLSDVIREAETAAVDAALRR
                     YGQTVEGKRLAAEALGISLATLYNKLG"
     gene            1477..>1743
                     /locus_tag="PLQ29_12365"
     CDS             1477..>1743
                     /locus_tag="PLQ29_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87482.1"
                     /translation="MLAVLGILTIALLLVLVMTKKASPVVALIIVPIVMALIGGFGAD
                     ITKFVTDGVKSIATTGVMFIFAILFFGVLTDAGTFQPIIKGILKV"
BASE COUNT          360 a          559 c          520 g          304 t
ORIGIN      
        1 ttcgctacgt taatccgtcc tatacccgta taaccgcggt cgaggagagc gacatcgtgg
       61 gcaagccgct cgccgaggcg cgccccggta gccacctgcc tacggtagtc gccactggcg
      121 tacaggagct cggcgtcagg cgcaagcagg gcaacgccga gtatatggtc aacatggtgc
      181 ccatatgccg cgccggcgtt atcataggcg gcatatcgat cctcaacgag gtcaacgacg
      241 tgtaccggct cgtcaaggag ctcgaccgct cgaaccgctt gatacagagg ctcaaggacc
      301 gagtacgcca gataggcaac gcgcgctaca gcttcgacga catcgtatcg aacgacgcgc
      361 gctcgcaggc cacgaaggac ctggccgccc ggctcgcccg gaaggacgtg aacctgctga
      421 tactcggcga gagcggcacg ggcaaggagc tgtacgcgca gtcgatacac aacgccagcc
      481 agcgcagcaa ccatccgttc ctcgccgtca actgcgccgc cctcgacccg aacctgctcg
      541 agagcgagct gttcggctac gaggacggat cgttcacggg cgcgaggaag ggcggcaaga
      601 taggcctctt cgaggaggcg tcgggaggca cgctcttcct ggacgagata ggcgagctcg
      661 acgtccggct ccaggccaag ctactccgcg cccttcagga gggcttgata cgcccggtcg
      721 ggggcgcgtc ggaggtatcc gtcgacgtcc gcgtcatggc agccacgaac aaggacatag
      781 agacgctggc gaaggacgga tcgttccggc gcgacctgta ttaccgcgta gccacgttca
      841 cgctcatcct gtcgccgctc agggagcgtc ggggcgacat tacccccctc gtccagaagt
      901 tcacgaacga tatagccggg cgctaccgcg cgaccctcga tatcgatccg gacgccatgg
      961 cggccctcga ggcatgggat tggccgggca acgtacgcga actcaagaat accatcgagt
     1021 acgcggctat catggccgag gacggccgca taagcgctag ggacctacca ccgcgtatgg
     1081 tacacggccg agcgggcacc gctccggacg aggtacgacc gctctccgac gtcatacgcg
     1141 aagcggagac cgcggccgtc gacgccgccc tccgtcgcta cggccagacg gtcgagggaa
     1201 aacgcctggc cgccgaggcc ttaggcatat cgctcgccac cctctacaac aagctcggct
     1261 gaaccgtccg tttccaggct tttagaatac ccaattccat ttttctagaa ttctacaaac
     1321 tccaacattg gtcgtaccgc gaaaggttca agggatttcg attcgttgcg acctgatcac
     1381 ggagattgcg aaggatcgta gcggatcggc ataggcgatt cgacttggca cgcttcttga
     1441 ttataaggga agtactaccc atttaaggag gctgccatgc tggcagtatt aggcatcctg
     1501 accatcgccc tgctcctggt cctggtcatg accaagaagg cgtcaccggt cgtggcgctc
     1561 atcatcgtcc cgatcgtgat ggccctgatc ggcggcttcg gcgccgacat cacgaagttc
     1621 gtcacggacg gcgtcaagag catcgccacg accggcgtca tgttcatctt cgccatcttg
     1681 ttcttcggcg tactgaccga cgccggcact ttccagccca tcatcaaggg catcctcaag
     1741 gtc
//
LOCUS       DAOPHV010000586         1742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_98214_length_1742_cov_3.804979, whole genome shotgun sequence.
ACCESSION   DAOPHV010000586 DAOPHV010000000
VERSION     DAOPHV010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98214_length_1742_cov_3.804979"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /gene="fliM"
                     /locus_tag="PLQ29_12370"
     CDS             complement(<1..924)
                     /gene="fliM"
                     /locus_tag="PLQ29_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HPG87483.1"
                     /translation="MTEVLSQDEIDQLLTAINAGDTSLETVQSATDSRKIKIYDFRRP
                     DKFSKEQMRTVQNMHETFARLTTTSLSASLRILVNVHVASVDQLTYEEFIRSIPTTTT
                     LAVVSMDPLKGQIILEIDPAVTFTIIDRLFGGAGEGSKLTRELTDIEHSVMEGIIVKI
                     LGNLREAWTQVIDLRPRLSQIEVNPQFAQIVPPTEMVVLVTLETKIGDVEGMMNLCIP
                     YLTIEPIISKLSAQYWYSSVRRGATTENLNILKEKLSTIDVPVIAEIGRIQVTVGDVL
                     NLNLGDIVRLHNVKVDDPMVLNVGNKKKNMKL"
     gene            complement(1002..1556)
                     /locus_tag="PLQ29_12375"
     CDS             complement(1002..1556)
                     /locus_tag="PLQ29_12375"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HPG87484.1"
                     /translation="MSDEDFDKAGPDFSDSPEVADKPKGKGGGKAILGLLKWIGIGLG
                     AVIFIIVIVVVTVSLLNRQAKPMTTVPTSEEYQRATPIWATFTSIDQITTSTIDKEPW
                     SVMIKLNLAYDQADKEIQTELTSRKYQIQDALRNFFSMKSIKELMPNREQELKEELRE
                     MLNRMLSKPAIKDVYFQTFSRNQM"
     gene            complement(1576..>1742)
                     /locus_tag="PLQ29_12380"
     CDS             complement(1576..>1742)
                     /locus_tag="PLQ29_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HPG87485.1"
                     /translation="ALGVLHYLSDFGVPEARFQVSGLAATMPIADDSTAEGRAYNRRV
                     DIIILDEGHL"
BASE COUNT          303 a          515 c          520 g          404 t
ORIGIN      
        1 cagcttcatg ttcttcttct tgttgccgac gttgagcacc atcgggtcgt cgaccttgac
       61 gttatgcagc ctgacgatgt cgcccaggtt caggttgagc acgtcgccta ccgttacctg
      121 gatgcggcca atttccgcta tcaccggcac gtctatcgtc gacaacttct ccttgagaat
      181 attcaggttc tcggtcgtgg cgccgcgtcg taccgacgag taccagtact gggccgacag
      241 cttggagatg atcggctcta tcgtcaggta ggggatgcag aggttcatca tgccctcgac
      301 gtcgccgatc ttcgtctcca gggtcacgag cacgaccatc tccgtcgggg gtacgatctg
      361 cgcgaactgc gggttgacct cgatttggga cagcctcggg cgcaggtcga tgacctgggt
      421 ccaggcctcg cgcaggttgc cgagtatctt gacgatgatg ccttccatga cggaatgctc
      481 gatatctgtc agctcacggg tcagcttgga gccctcgccc gcgccgccga acaggcgatc
      541 gatgatggtg aacgtgaccg ccgggtctat ttccaggatg atctggccct tgagcggatc
      601 catactgacg accgcgaggg tcgtggtcgt cgggatcgac ctgatgaatt cctcgtaggt
      661 cagctggtcc accgacgcga cgtgtacgtt gaccaggata cgtaacgacg cggacaggct
      721 cgtggtcgtc aggcgcgcga aggtctcgtg catgttctgc acggtgcgca tctgctcctt
      781 cgagaactta tccgggcgcc tgaagtcgta gatcttgatc ttgcgcgagt cggtcgccga
      841 ctgtaccgtc tcgagcgacg tatcgccggc gttgatggcg gtgaggagct ggtctatctc
      901 gtcctgggac aggacttcgg tcatctgttc actccgcctg ctatcattgc atagaaaaac
      961 gcttttagca acgtcgcggg cccatccgcg acgtcgaacg cctacatctg attacgagag
     1021 aaggtctgga aatacacgtc cttgatcgct ggcttcgaaa gcatgcggtt gagcatctcc
     1081 cgtagctcct ccttgagctc ctgctcgcgg ttgggcatta gctccttgat gctcttcata
     1141 ctgaagaaat tgcgcagcgc atcctggatc tggtacttgc gcgaggtcag ctcggtctgg
     1201 atttccttgt cggcctggtc gtaggccaag ttcagcttga tcatgaccga ccagggctcc
     1261 ttgtcgatcg tgctcgtcgt gatctggtcg atcgacgtga aggtggccca gatcggggtg
     1321 gcgcgctggt attcctccga ggtcggcacg gtcgtcatag gcttggcctg ccgattgagg
     1381 agactcacgg tgacgacgac tatgacgatg atgaagatga ccgcgccgag gcctataccg
     1441 atccacttga gcaggccgag tatagccttg ccgccgccct tgcccttcgg cttgtccgcg
     1501 acctcggggg agtcgctgaa atccggtccg gccttgtcga aatcttcgtc gctcatatgg
     1561 cgctacctcc ggtcatcata gatggccctc gtccagtatt ataatgtcaa cgcgccggtt
     1621 gtacgcccgg ccctccgctg tagaatcgtc ggctatcggc atcgtcgccg cgaggcccga
     1681 cacctggaaa cgcgcctccg gcaccccgaa gtcgctcagg tagtgtagca cgccgagggc
     1741 cc
//
LOCUS       DAOPHV010000587         1738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_98566_length_1738_cov_4.128936, whole genome shotgun sequence.
ACCESSION   DAOPHV010000587 DAOPHV010000000
VERSION     DAOPHV010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98566_length_1738_cov_4.128936"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1198)
                     /locus_tag="PLQ29_12385"
     CDS             complement(5..1198)
                     /locus_tag="PLQ29_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HPG87486.1"
                     /translation="MAKPLEGVRVLDLTRVLAGPFCTMVLSDLGAEIIKVEVPGTGDD
                     SRAFGPFKNGQSLYFVNINRGKKSVAIDLKTDAGKKLLVDLAKECDVIVENFRPGTME
                     KLGLGWDVLKEANPRLIYAAVSGFGHTGPDSLRPAYDILVQAMGGVMSITGWPESPPT
                     RVGLSMGDITAAIFCAVGVNAALFQRQKTGLGQKVDVSMLDCQLSILENALSRYQVDG
                     RSPSPLGTRHPTITPFQAFRAADGWFVIAVGNDALWRKFCDAMGRRDLLEDPRFATNG
                     DRTKHYDDLIPELERLFESKDRAAWLETLEKAGVPSAPVNTVKDVMADRQLAARNMLV
                     TVEDDRAGTLTIPGNPIKMESVPESPDRPKAPAIGEHTASVLRDLLGLDEAAAASLRD
                     QGVVV"
     gene            complement(1350..>1738)
                     /locus_tag="PLQ29_12390"
     CDS             complement(1350..>1738)
                     /locus_tag="PLQ29_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPG87487.1"
                     /translation="EGEEDGAERVEQAAECDAHLKKKYGKGLSEVFADDKYQLMHIEM
                     FNVGIIHAECLGGDIDLLLNQRATIGCFPWRFVDGESSIARIVAMVDDERYEKLQAKK
                     AKCELTKFGDIAGAKAAWLHEEARKR"
BASE COUNT          277 a          557 c          553 g          351 t
ORIGIN      
        1 gcgttcatac gacgacgccc tggtcgcgca gggacgcggc ggccgcttcg tcgaggccga
       61 gcaggtcgcg cagcacgctg gcggtatgct cgcctatggc cggcgccttg ggcctgtccg
      121 ggctctcggg cacgctctcc atcttgatcg ggttgccggg gatggttaat gtgccggccc
      181 tatcgtcctc gacggtgacg agcatgttgc gcgccgcgag ctgacggtcg gccatcacgt
      241 ctttgacggt gttcaccggg gcgctcggca cgccggcttt ctccagcgtc tcgagccagg
      301 cggcgcggtc cttcgactcg aacaggcgct cgagctcggg gatcaggtcg tcgtagtgct
      361 tggttcggtc gccgttggtc gcgaagcgcg gatcctcgag caggtcccgc cgccccatcg
      421 cgtcgcagaa tttcctccag agcgcgtcgt tgccgacggc tatgacgaac cagccgtcgg
      481 ccgcgcggaa agcctggaat ggggtgatgg tcgggtggcg ggtgccgagc ggcgacggcg
      541 agcggccgtc gacctggtag cgcgacagcg cgttctcgag gatggacagc tggcaatcca
      601 acatcgacac gtccaccttc tgcccgaggc cggttttctg gcgctggaag agcgcagcgt
      661 tgacgccgac ggcgcagaat atagccgcgg tgatgtcgcc catcgacagc ccgacgcggg
      721 tgggcggaga ttccggccac ccggtgatgc tcatcacgcc gcccatggcc tgtacgagga
      781 tatcgtaggc cggccggagc gagtcggggc cggtatggcc gaaaccggag acggcggcgt
      841 agatcagcct cggattggct tccttgagca cgtcccagcc gagcccgagc ttctccatcg
      901 tccccggcct gaagttctcg acgatcacgt cgcattcctt ggccaggtcg acgagcagct
      961 tcttgccggc gtcggttttg agatcgatcg cgacgctctt cttgccgcgg ttgatgttga
     1021 cgaagtagag gctctggccg ttcttgaacg gcccaaaggc gcgagaatcg tcgccggtac
     1081 ccgggacctc gaccttgatg atctccgctc ccagatccga aagcaccatc gtgcagaacg
     1141 gtcctgccag tacccgggta agatcgagga cgcgcacgcc ctccagcggc tttgccatac
     1201 gtgctcctgg tcattgataa ccgattaaga atatcacgga ggcacggagg cacggagaat
     1261 gataagaaaa gataaggacc aggttttcct agcctattct tgaatccttc ttatctccgc
     1321 gtctccgtgc ctccgtggcc atattcgcct tagcgcttgc gggcttcctc atggagccag
     1381 gcggccttgg cgccggcgat gtctccgaac ttcgtcaact cgcacttggc cttcttggcc
     1441 tgcagtttct cgtagcgctc gtcgtctacc atggccacga tgcgcgcgat actcgactcg
     1501 ccgtcgacga agcgccacgg gaagcagcct atcgtcgccc tctggttcag gagcaggtcg
     1561 atgtcgccgc ccaggcactc ggcgtggatg atgccgacgt tgaacatctc gatgtgcatc
     1621 agctggtact tgtcgtcggc gaatacctcg gatagtccct tgccgtactt cttcttcagg
     1681 tgggcgtcgc actcggcggc ctgctctaca cgctcggcgc catcctcttc gccctcca
//
LOCUS       DAOPHV010000588         1738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_98605_length_1738_cov_2.511586, whole genome shotgun sequence.
ACCESSION   DAOPHV010000588 DAOPHV010000000
VERSION     DAOPHV010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98605_length_1738_cov_2.511586"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1414
                     /gene="rsxC"
                     /locus_tag="PLQ29_12395"
     CDS             68..1414
                     /gene="rsxC"
                     /locus_tag="PLQ29_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPG87488.1"
                     /translation="MVTKTFKGGTHPPERKERSASTPIQTLRNVSQVVIPVNQHFGAP
                     IQPLVKVGDQVKRGQKIADADGRMTVPLHASVYGTVKKIEPRMQANNLEGLAIVIEPL
                     PGDEAVFMPTLDPFSCTKEEALARVREAGIIGVGGAGFPAHVKFAPPPNKPIEYVIAN
                     AAECEPYLTIDERVMEEHPEDFIDGIAIVMKIVGATKGIVGLEDNKTHLVPGLEKAIA
                     AKRSGMDIRVQLLRTKYPQGGEKMLITAITGREVPSGGLPMDVGCVVSNVNTLLAVSE
                     AFRKGQPVIDTGLTVTGGACETPKNLFVPVGMLVQDLFPDVARYDEKKLKRIVMGGPM
                     MGPAVPSMLVPIQKNTSGVLLMDETEAKAYEEGPCIRCGRCMRACSCRLSPALLNVAL
                     KAGDLDEADKIGVMDCIECGTCSFVCPSHIQLVQRFRVGKQLVRAKKQKEAQRAGK"
     gene            1401..1673
                     /locus_tag="PLQ29_12400"
     CDS             1401..1673
                     /locus_tag="PLQ29_12400"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPG87489.1"
                     /translation="MPANNMLLSSSPHVYTGVTPRKLMLSVIIALSPVSVYGVVLYGV
                     HALLVIVVSIATARVLGFAAAGRAAKVTTATTIMNTSSRGRFTEKS"
BASE COUNT          340 a          568 c          552 g          278 t
ORIGIN      
        1 gccccaaagt cagcgtcgcg gagcctaggc cccgcggtag caacggaaga aggagcccct
       61 cctcgccatg gtgacgaaaa cattcaaagg cggaacgcat ccgccagagc gcaaggaacg
      121 aagcgcatct acgccgatac agaccttgcg caacgtatcg caggtggtca tcccggtcaa
      181 tcagcacttc ggcgcgccta tccagcccct cgtcaaggtc ggggaccaag taaagcgcgg
      241 tcagaagatc gcggacgccg acgggcgcat gaccgtcccg ctccacgcgt cggtctacgg
      301 caccgtcaag aagatcgagc cgcgcatgca ggctaacaac ctcgagggcc tggccatcgt
      361 catcgagccc ctgcccggcg acgaagcggt gttcatgccg acgctcgatc ccttcagctg
      421 cacgaaggaa gaagcgctcg ctcgcgtacg cgaagcgggt atcatcggcg tcggcggcgc
      481 tgggtttccg gctcatgtca aattcgctcc gccgccgaac aagccgatcg agtacgtgat
      541 agcgaacgcg gccgagtgcg agccctacct gacgatagac gagcgcgtga tggaggagca
      601 tcccgaggac ttcatcgacg gcatcgccat cgtcatgaag attgtcggcg cgaccaaggg
      661 catcgtcggg ctcgaggaca ataagaccca cctcgtgccc gggctcgaga aggccatcgc
      721 cgccaagcgc tcgggcatgg acatccgcgt ccagctcctc aggaccaagt accctcaggg
      781 cggagagaag atgctcatta cggccattac cggccgagag gtgccgtccg gcggcctgcc
      841 gatggatgtc ggctgcgtcg tcagcaacgt caacacgcta ctcgccgtgt ccgaggcttt
      901 ccgcaagggc cagccagtca tcgataccgg cctgaccgta accggtggcg cctgcgagac
      961 tccgaagaac ctattcgtcc ccgtgggcat gctcgtccag gacttgttcc ccgacgtggc
     1021 ccggtacgac gagaagaagc tcaagcgcat cgtgatgggc ggcccgatga tggggccggc
     1081 cgtaccgtcg atgctcgtgc cgatccagaa gaatacctcc ggcgtgctcc tgatggacga
     1141 gacggaggcc aaggcctacg aggaaggccc gtgcatccgt tgcggccggt gcatgcgcgc
     1201 ctgctcgtgc aggctgtcgc cggccctcct caacgtggcc ctcaaggcgg gcgacctcga
     1261 cgaggctgac aagataggcg tcatggactg catcgagtgc ggtacctgca gcttcgtctg
     1321 cccgtcccat atccagctcg tccagcgctt ccgcgtaggc aagcagctcg tccgggccaa
     1381 gaagcagaag gaggctcagc gtgccggcaa ataatatgct cctctcctcg agcccgcacg
     1441 tatataccgg ggtgacgccg aggaagctga tgctgtcggt gatcatagct ctgtccccgg
     1501 tgtcggtgta cggcgtggtg ctctacgggg tgcacgcgct cctcgtcatc gtcgtctcga
     1561 tcgcgaccgc gcgggtcttg ggctttgccg ccgcggggcg cgccgcgaag gtgaccaccg
     1621 cgacgaccat catgaacacc agcagcaggg gacgcttcac cgaaaaatca tagcagcggc
     1681 ggcggcggat ccttggccgg cgcctggcac gtctccatgt acgatgacgc cgcggatg
//
LOCUS       DAOPHV010000589         1737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_98672_length_1737_cov_3.056480, whole genome shotgun sequence.
ACCESSION   DAOPHV010000589 DAOPHV010000000
VERSION     DAOPHV010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98672_length_1737_cov_3.056480"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="PLQ29_12405"
     CDS             <1..1509
                     /locus_tag="PLQ29_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87490.1"
                     /translation="PDPFLNSVQGDDEVEVLVFKMAVALGFDAPRAFTLVSFRSSRDP
                     DFGLQIVGRLMRVDRRLQRILPVAPELMHGYVFLSDKSSQEGIVTAGDRINAIRNELA
                     SLETSVDVIALGTDDAALVSVRNGQPELPGVSYGSDHAGFPAGEPRQPAYAERSLFAE
                     WGLTPGGTGSGSIAKVPAAVSRPVQASSGFSWALKQSAPRAFTRAEVDVLSFDALTDI
                     YNRFRWDADTLLLAKIDSGKVLMERYEVFSHLREAPEEIRARLFKDELSRRSQLCLRN
                     ADRDGFLDQRALYAGLMDRLAAEASDKGISGLESREELEDGLAHILALRPDLLTKAVS
                     ESFAAHTVSVQADDLPSAIVSSEPLIGSRLNIYGVYPSDLNNWERPFAERLDNDLSGA
                     VLWWHRNPVHKRHSVSLPVAGHGFYFPDFIVGVKGRSTPGGVILVEIKYQINDPIGNA
                     AAKSRACHPDYGSVLMLYLDDKSGQWMTVVYDERKGQNVLDRVFREDQLVTF"
BASE COUNT          325 a          554 c          520 g          338 t
ORIGIN      
        1 cccgacccct tcctcaactc ggtgcagggc gacgacgagg tcgaggtcct ggtcttcaaa
       61 atggcggtcg ccctcggctt cgacgcgccg cgcgccttta cgctggtcag cttccgttcc
      121 agccgcgacc cggacttcgg cctccagatc gtcggccgcc tcatgcgcgt cgaccgccgg
      181 ctccaacgta tcctgcccgt ggcgccagag ctgatgcacg gctacgtctt cctctccgac
      241 aagtcgtccc aggagggcat cgtcacggcg ggagaccgca tcaacgccat caggaacgaa
      301 ctggcctcgc tcgagacgtc ggtggacgtc atagccctcg gaacggacga cgccgcgctg
      361 gtgagcgtgc ggaatggcca gcctgagcta cccggcgtaa gctatgggag cgatcacgcc
      421 ggcttcccgg ctggcgagcc tcgtcagccg gcctacgccg aacgatcgct gttcgccgaa
      481 tggggcttga ctccgggcgg gaccggctcc ggttccatcg ctaaggtacc cgcggccgta
      541 tctcggcccg tccaagcttc atccggtttc agctgggccc tcaagcagtc cgcgccgcgc
      601 gcctttaccc gcgccgaggt ggatgtgctg tctttcgacg cactgacgga catctataac
      661 cggttcaggt gggacgccga tacgctgttg ctcgccaaga tcgatagcgg caaggtgctg
      721 atggagcgtt acgaggtctt tagccacctg cgggaggctc ccgaggagat acgggcgagg
      781 ctgttcaagg acgagctgtc gcgacgttcg caactatgcc tgagaaacgc ggaccgggac
      841 ggcttcctgg atcagcgagc cctctacgcc ggcttgatgg atcggctcgc ggccgaggcg
      901 tccgacaagg gtatatccgg gttggagagc cgcgaagaac ttgaagacgg gttggcgcat
      961 atactcgcgc tcaggccgga cctcctgacc aaggctgtca gcgaatcctt cgccgcgcat
     1021 acggtttcag tccaggctga cgaccttccg tccgctatcg tatcctccga gccgctcatc
     1081 ggttcccggc tcaatatcta tggcgtgtac ccttcggacc tgaataactg ggagcggcca
     1141 ttcgccgagc gcctggacaa cgacctgtcg ggcgccgtgc tctggtggca ccggaatcct
     1201 gtgcacaaga ggcattcagt gtctttgccg gtggccggcc acggtttcta cttccccgat
     1261 ttcatcgtcg gcgtcaaggg ccgctccacg ccgggcggcg tcatcctcgt ggagatcaag
     1321 taccagataa acgacccgat aggcaacgcc gcggccaaga gccgcgcctg tcacccggac
     1381 tacggttctg tcttgatgct ctacctcgac gataagagcg gccagtggat gaccgtcgtc
     1441 tacgacgagc gtaaaggcca gaacgtcctc gaccgcgtgt tccgcgagga ccagctcgtc
     1501 acgttctagg ccaggaccaa cgttatcttc cagtcgagtc tccgcataac acgacggtgt
     1561 cagtatttta tcggatcatg aacgagcatc atccggaagc gcttgagtat ctctaggatg
     1621 cgactgaata gtacgcaggc caacgccggc ccgtttacga gtaccgttag ctgatactct
     1681 acctagggct tcctgaacaa catctaagaa tttacaagac aaggaaatag ttggtat
//
LOCUS       DAOPHV010000590         1734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_98913_length_1734_cov_3.127457, whole genome shotgun sequence.
ACCESSION   DAOPHV010000590 DAOPHV010000000
VERSION     DAOPHV010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98913_length_1734_cov_3.127457"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PLQ29_12410"
     CDS             complement(<1..511)
                     /locus_tag="PLQ29_12410"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPG87491.1"
                     /translation="MATLSFSIPRDIVYGENALERLSTLRGTRAVLVTGGSSMKKFGF
                     LDKAAALLAKAGIESTVIDGVEPNPSVQTVRRGAEAMLTFKPDWIVAIGGGSAIDAAK
                     VMWCFYEHPGVKFEDIITPGSMPSLRNKARFVAIPSTSGTASEITAFSVITDTEKHIK
                     YPIVAADMVP"
     gene            661..>1734
                     /gene="mnmE"
                     /locus_tag="PLQ29_12415"
     CDS             661..>1734
                     /gene="mnmE"
                     /locus_tag="PLQ29_12415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPG87492.1"
                     /translation="MAKGYLDIEDDIAAIATAPGTAALAVVRVAGPGSVERAARAFSR
                     PDALKSLGGYRAAYGKLVDPDGRAVDEVVALVFRGPVSYTGQDGVDLMCHGGRATTDG
                     ALSALEAVGFRRALPGEFTFRAFYNGKLDLARAEAVGELVAARTAASRADAFSRLSGG
                     LSRELAAIKASLVRMAAAFALRLDYGEDEAPEDIAAELPALRAAREACLALTDSYAVG
                     RALLDGSVVALAGAVNAGKSSLFNRLLKDERAIVSSEPGTTRDYLEAELDLGGLPVRL
                     VDTAGVRDSADPIELEGVRRSRLVAGGADAVVYVVDATAGLRPDDLAFVEANPAAIRA
                     WNKIDEAGATKAPAGWISVSAETG"
BASE COUNT          266 a          578 c          612 g          278 t
ORIGIN      
        1 cgggcaccat gtccgcggcg acgatcgggt acttgatgtg cttctcggta tcggtgatca
       61 ccgagaacgc ggtgatctcg ctcgcggtgc cgctcgtcga cgggatcgcg acgaagcgcg
      121 ccttgttcct gagcgacggc atcgagccgg gggtgatgat gtcctcgaac ttgacgccgg
      181 ggtgctcgta gaagcaccac atcaccttgg cggcgtctat ggcggacccg ccgccgatgg
      241 cgacgatcca gtcgggcttg aacgtgagca tcgcctcggc gccgcgcctg acggtctgca
      301 cggacgggtt cggctcgacg ccgtctatga ccgtgctctc gatgccggcc ttggcgagca
      361 gcgcggcggc cttgtccagg aagccgaatt tcttcatcga cgaaccgccg gtaacgagta
      421 cggccctcgt tcccctcagg gtcgagaggc gctcgagcgc gttctctcca tatacgatgt
      481 cccgcgggat cgagaatgaa agcgtagcca tggtgacctc ctagtcgctc agtagcgata
      541 aaacttatcg ataaaagaat acttaaaatg tacaagagtg tcaagcgcgc gatcggctcg
      601 cctcgcggat cgcgcgcgta tcacttgcgg gtcccggccg gaacgcgcta tcgttgcccg
      661 atggccaagg gatacctgga tatagaggac gacatagccg cgatagcgac ggcgccggga
      721 acggccgcct tggcggtggt tcgcgtcgcg gggccgggca gcgtcgagcg cgcggcccgc
      781 gccttctccc gcccggacgc gctcaagtcg ctcggcgggt accgggccgc gtacggtaag
      841 ctggtcgacc ccgacggtcg cgcggtcgat gaggtcgtgg cgctcgtctt cagggggccg
      901 gtgagctata caggtcagga cggtgtcgac ctcatgtgcc acggcggccg cgccacgacc
      961 gacggcgcgc tgtcggccct cgaggcggtc ggcttcaggc gcgccctgcc cggtgagttc
     1021 acgttcaggg ccttctacaa cggcaagctt gacctggccc gggccgaggc cgtaggcgag
     1081 ctcgtggcgg cgcgtaccgc ggcctccagg gccgatgcgt tctcaagact gtccggcggc
     1141 ctgtcgcggg aactcgcggc catcaaggcg tcgctcgtca ggatggccgc cgcattcgcc
     1201 ctgcgcctcg actacggcga ggacgaggcg cccgaggaca tcgcggcgga gctgccggcc
     1261 ctgcgcgccg cccgcgaggc ctgcctggcg ctgaccgaca gctacgccgt cggtcgggcc
     1321 ctgctcgatg gctcggttgt agcgttggcc ggggccgtca acgccggcaa gtcgtcgctg
     1381 ttcaaccgcc tactcaagga cgagcgcgcc atcgtatcga gcgagccggg caccacccgc
     1441 gactacctcg aggccgagct cgacctgggc ggcctgccgg tacgcctcgt cgatacggcc
     1501 ggcgtgcgcg acagcgccga cccgatagag ctcgagggcg tcaggcgctc gaggctcgtg
     1561 gcgggcgggg ccgacgccgt cgtctacgtg gtggacgcga ccgccggcct acggcccgac
     1621 gacctggcct tcgtcgaggc gaatccggcg gcgatcaggg cctggaacaa gatcgacgag
     1681 gccggtgcga ccaaggcccc ggccggctgg atatccgtgt cggccgagac cggc
//
LOCUS       DAOPHV010000591         1731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_99126_length_1731_cov_4.676014, whole genome shotgun sequence.
ACCESSION   DAOPHV010000591 DAOPHV010000000
VERSION     DAOPHV010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99126_length_1731_cov_4.676014"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="PLQ29_12420"
     CDS             complement(<1..1189)
                     /locus_tag="PLQ29_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87493.1"
                     /translation="MSRFSRLFASIAASSLLCLPCLATLACSGGAPGASEARSSSRTP
                     MRAVAVSPEVDEPRVLAALSARGIDDALVASRVMVPFSDFSRVVDAPLDSALRRSRDA
                     DPRRTPFLEELASRFTATGPDGSAWTVVYLPGPTAERDEAAALSLTSLGADWAWDARS
                     GAHSSPILWLPAATFALWLVVRKPKSGRTARLVVALASSPLLAPGAIGASTLFVSLTA
                     AFAAAYGVARSAPTRRRDHSRAILKALWPYGFAIAAPLAAIVALERSSLPYLAASAAL
                     AALSVALYPRLAKVSASRRLHGAPRFVALTGSRTTRESRSVAKAMLAPVLAMAAMQPF
                     ASTGVYRAGDASPGPAFSLTRSQRGVEDRTDAESLLAEHLAYQEALTYGRIGDAAWGR
                     SGYD"
     gene            complement(1193..1672)
                     /locus_tag="PLQ29_12425"
     CDS             complement(1193..1672)
                     /locus_tag="PLQ29_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HPG87494.1"
                     /translation="MEHQRVFFISALLRNNVFLAAALSLFMAQFAKALVAVFTNRKAG
                     IREIFSTLLWKTGGMPSSHSALVIAISTSIAFSSGIGSDVFALSFFFALVVIRDAMGV
                     RRAAGVQAKTLNSLGSRLSKRLHIPFKPVKEINGHTFPQVVAGSLMGFFIALAVNTL"
BASE COUNT          317 a          546 c          623 g          245 t
ORIGIN      
        1 gatcgtagcc gctccgcccc caggcggcgt cgccgatgcg cccataggtc agcgcctcct
       61 ggtaggccag gtgctcggcg agcagcgact cggcgtccgt tcggtcttcg acgccgcgct
      121 gggacctcgt cagcgaaaag gccggaccgg gcgatgcgtc gccagcgcgg tagaccccgg
      181 tcgacgcgaa gggttgcatg gcggccatgg ccaggacggg cgccagcatg gccttggcca
      241 cggaacgcga ctcccgcgtc gtcctcgatc cggtaagcgc gacgaaccga ggcgccccgt
      301 gcagccgcct cgacgccgaa accttcgcca gcctcggata gagcgccaca ctgagcgccg
      361 ccagggccgc tgaggccgcc aggtacggca ggctcgagcg ctccagggcg acgatggccg
      421 cgagcggagc ggcgatcgcg aacccgtacg gccagagcgc cttgaggatt gctctcgagt
      481 ggtctcgcct tcgggtagga gcgctccgcg cgacgccgta tgccgccgcg aaggcggccg
      541 ttagcgagac gaacagcgtg gaggcgccga tcgcgccggg cgcgaggagc ggcgacgatg
      601 cgagggcgac gacgagcctg gccgtccttc cgctcttcgg cttcctgacg acgagccaca
      661 gcgcgaacgt cgcggccggg agccagagta tcggggacga gtgcgcgccg gagcgggcgt
      721 cccaggccca gtcggctccg agcgacgtta gggacagagc cgcggcctcg tcgcgctcgg
      781 ccgtcggccc gggtaggtag acgaccgtcc aggccgagcc gtcggggccg gtcgccgtga
      841 acctcgaggc cagctcctcg aggaagggcg tacggcgcgg gtcggcgtcg cgggagcggc
      901 gcagggcgct gtcgagaggc gcgtcgacga cgcgggagaa gtcgctgaag gggaccatga
      961 ccctcgaggc taccagcgcg tcatcgatgc ctcgggccga gagggcggcc agtacgcgcg
     1021 gctcgtcgac ctcgggcgag acggccacgg cgcgcatcgg ggtgcgcgac gacgagcgcg
     1081 cctcggaggc cccgggagcg ccacccgagc aggcgagcgt ggccaggcaa ggcaagcaga
     1141 gcaggctgga ggcggctatc gatgcgaaga ggcgcgagaa ccggctcacg cgctacagcg
     1201 tgttgacggc cagggctatg aagaagccca tgaggcttcc ggcgacgacc tgcgggaagg
     1261 tatgcccatt gatctccttg accggcttga aggggatatg gaggcgtttc gatagcctgg
     1321 agccgagcga gttgagggtt ttagcctgta cgccagccgc gcgcctgacg cccatcgcgt
     1381 cgcgtatgac caccagggcg aaaaagaacg acagcgcgaa cacatccgag cctattccgc
     1441 ttgaaaatgc aatcgaggtg gatatggcta tgaccagggc gctgtggctc gagggcatac
     1501 cgccggtctt ccataggagc gtagaaaata tctcgcggat accggccttg cggttcgtga
     1561 agaccgcgac gagagccttg gcgaattgcg ccatgaagag gcttaacgcc gccgccagga
     1621 acacgttatt cctgagtaaa gcgcttataa aaaatactcg ctggtgttcc atcgcgagaa
     1681 tttcaagtat aatcgcttct cttgtcaagc ctatacgagt gtcaagccta t
//
LOCUS       DAOPHV010000592         1731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_99159_length_1731_cov_2.931981, whole genome shotgun sequence.
ACCESSION   DAOPHV010000592 DAOPHV010000000
VERSION     DAOPHV010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99159_length_1731_cov_2.931981"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(303..1466)
                     /locus_tag="PLQ29_12430"
     CDS             complement(303..1466)
                     /locus_tag="PLQ29_12430"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG87495.1"
                     /translation="MHEYIDRFITKAVRDDLRLFPAVALVGPRQCGKSSLAARITEDD
                     PTALMLDLENPRDRAKLADPLLYLEANASRLVCIDEIQLLPELFPVLRAEIDRDRRPG
                     RFLLLGSASRELVNRSSESLAGRIGYQELSPFLPTEYVAGSPPSMLESLCRGGFPESA
                     LAADSQASYRWREAFVRSVVERDLPMLGSRIPAVSMERFLTMCAHLQGQVLNAAKLGS
                     SLDLTGPAVRARLEFLSSALLVRLLPSWEGNLKKRLVKAPKLYLRDTGLCHALLGIES
                     VDDLLGHPGFGASWEAWCVEALCSSLPGWKASFYRSSGGAELDLILERGRRRLAFEFK
                     ASSAPSAGKGLYSAMSDLEPERCFVVGLIEGSYPLARNVVACGLRECIGLIQG"
BASE COUNT          365 a          512 c          528 g          326 t
ORIGIN      
        1 acattccggc cgtcacgtgg accacgaccg gccataccgc aacgcccgtg gacctgtacg
       61 catacggcgc aggctccgaa cgtttcctga ctatattgga caacaccgat atccacgctg
      121 ccctgctcga gctactagac taggcgaaga cgtgacatac tcgagggaat gaacgagccg
      181 ctcgagtcca gttgcgggtc ggttgatctg taaggtggga agtgcgctac gcctcccatg
      241 cccttaagct ttcatcttga tcatcctgtt ccgtctttat cctatccctt ggcgtctccg
      301 cgttagccct gtatcaggcc tatgcactcg cggaggccgc aggcgactac gttccgcgcc
      361 agggggtagc tgccttctat gaggcctacc acgaagcatc gttccggttc caggtcggac
      421 atggccgagt atagcccctt gcctgcgctc ggcgcgctcg aagccttgaa ctcgaaggcc
      481 agccggcgtc ggccgcgttc aaggataagg tctagctcgg ctccgccgga gctacgatag
      541 aacgacgctt tccatcccgg caggctcgag catagggctt ccacgcacca ggcttcccag
      601 ctcgccccga agcccggatg cccgagcagg tcgtcgacgg actcgatgcc gagcagcgcg
      661 tggcatagcc cggtatcgcg caggtatagc ttgggagcct tgacgaggcg tttcttcagg
      721 ttgccttccc atgacggcaa gagccgcacg agcagcgcag aggaaaggaa ttccagcctg
      781 gcccgtaccg cgggtcccgt caggtcgagc gacgatccta gcttggcggc gttcaggacc
      841 tgcccttgca ggtgggcgca catggtcagg aagcgctcca tggataccgc cggtatcctg
      901 gagccgagca tcggcaggtc ccgttctacg acgctcctga caaaggcttc ccgccaccgg
      961 tagctggctt ggctgtcggc tgccaaggcg gattccggga acccgcctcg gcagagcgac
     1021 tcgagcatcg aaggcgggga gcccgcgacg tactcggtcg gcaggaaggg cgaaagctcc
     1081 tgatagccta tacgcccggc taggctctcg gagctacggt tgacgagctc gcgggaggct
     1141 gaacccagga gcaggaagcg ccccgggcgg cggtcgcggt ctatctcggc gcgcaggacc
     1201 gggaataact ccggaaggag ctgtatctcg tcgatacaga cgagccgcga cgcgttggcc
     1261 tcgaggtaca ggagaggatc ggcaagcttg gccctgtcgc gagggttctc gaggtcgagc
     1321 atgagggccg tcggatcgtc ctccgtaatc ctggccgcga ggctggactt tccgcactgc
     1381 ctcggaccga cgagcgccac cgccggaaag agacgtagat cgtcacgcac ggccttcgta
     1441 atgaaacgat cgatgtattc atgcatatct aaagtgtagc tttagatgtg caatgaatca
     1501 agctgcctcg caaggaggag tcctaggcga agaagggaac atgatggtac aggatggaag
     1561 aagatgaaca agataaagca aagtccaggt ttaggacgta tcctgatcat cctgtcttca
     1621 tcttgatcat cctgttccgc ctttacccca ttcctaggcg aagacaagaa catgattaca
     1681 ggatggaaga agatgaacaa gataaagcaa agtccaggtt taggacgtat c
//
LOCUS       DAOPHV010000593         1729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_99318_length_1729_cov_3.029271, whole genome shotgun sequence.
ACCESSION   DAOPHV010000593 DAOPHV010000000
VERSION     DAOPHV010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99318_length_1729_cov_3.029271"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..>1729
                     /gene="fusA"
                     /locus_tag="PLQ29_12435"
     CDS             88..>1729
                     /gene="fusA"
                     /locus_tag="PLQ29_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPG87496.1"
                     /translation="MAFEISKMRNIGISAHIDSGKTTLSERILFYCNKIHAIHEVRGK
                     DGVGATMDSMELERERGITIASAATNVQWKDSPINLIDTPGHVDFTIEVERSLRVLDG
                     AVLVLCSVGGVQSQTLTVDRQLKRYHVPRLAFINKADRTGANPYRVKNQIVEKLGLNA
                     AFITLPIGLEDKLEGLVDLVQMKAVYFDGEGGVNLRYAEIPAHLQADAAKYREELLDC
                     ASMFSDELAEAYLEGAETPEMIKAAIRKGTVAEQFVPVFVGSAYKNKGVQLLLDGVVD
                     YLPNPTEVKNIALDLDQGEKEVVVQPDAKLPTVALAFKLEDGQYGQLTYVRIYQGEIK
                     KGSELFNTRARKKFKVGRLVRMHSATMEDIVEGGPGDIVALFGIECASGDTFCDPSLN
                     YAMTSMFVPDPVISLAIEPADKKSADQMSKALNRFTKEDPTFQTYVDPESNQTIIKGM
                     GELHLDVYIERMRREYKCEVTTGMPQVAYREAISQRAEFNYTHKKQTGGSGQYGRVAG
                     YMEPIEGAVYEFVDSVKGGAIPNEYIPSCDKGFKEAAKK"
BASE COUNT          353 a          568 c          559 g          249 t
ORIGIN      
        1 ctcgcgatcc aactacagaa cgacgtaact cgaggacggc gccggtccgg cgcggaccat
       61 accctgtgcg aaggtagagg caagagcatg gctttcgaaa tttcgaagat gcgtaatatc
      121 ggcatcagcg cccacatcga ctccggcaag acgacgctct cggagcgcat cctgttctat
      181 tgcaacaaga tccacgcgat ccacgaggtg cgcggcaagg acggcgtcgg cgcgacgatg
      241 gactcgatgg agctcgagcg cgagcgcggc atcacgatcg cctcggccgc gacgaacgtg
      301 cagtggaagg actcgccgat caacctgatc gacacccccg gccacgtcga cttcaccatc
      361 gaggtggagc gctcgctgcg cgtgctcgac ggcgcggtgc tcgtgctgtg ctcggtcggc
      421 ggcgtgcagt cccagaccct gacggtcgac cgccagctca agcgctacca cgtgccccgc
      481 ctggccttca tcaacaaggc cgaccgcacg ggcgccaacc cctaccgcgt caagaaccag
      541 atcgtggaga agctcggcct gaacgccgcc ttcatcaccc tgccgatcgg cctcgaggac
      601 aagctcgagg gcctcgtcga cctggtgcag atgaaggccg tctacttcga cggcgagggc
      661 ggcgtgaacc tgcgctacgc cgagatcccg gcccacctgc aggccgacgc ggccaagtac
      721 cgcgaggagc tgctcgactg cgcgtcgatg ttctccgacg agctggccga ggcctacctc
      781 gagggcgccg agacgccgga gatgatcaag gccgcgatcc gcaagggcac ggtggccgag
      841 cagttcgtgc cggtcttcgt cggctccgcc tataagaaca agggcgtgca gctgctgctc
      901 gacggcgtgg tcgactacct gccgaacccg accgaggtca agaacatagc gctcgacctg
      961 gaccagggcg agaaagaggt cgtggtccag ccggacgcca agctgccgac ggtggccctg
     1021 gcgttcaagc tcgaggacgg ccagtacggc cagctgacct acgtgcgcat ctaccagggc
     1081 gagatcaaga agggctccga gctgttcaat acgcgcgccc gcaagaagtt caaggtcgga
     1141 cgcctcgtgc gcatgcattc ggcgacgatg gaggacatcg tcgagggcgg ccccggcgac
     1201 atcgtggcgc tgttcggcat cgagtgcgcc tccggcgaca ccttctgcga cccgtcgctc
     1261 aactacgcga tgaccagcat gttcgtgccg gacccggtga tctcgctggc catcgagccg
     1321 gccgacaaga agagcgccga ccagatgtcc aaggcgctca accgcttcac caaggaagac
     1381 ccgaccttcc agacctacgt cgacccggag tcgaaccaga ccatcatcaa gggcatgggc
     1441 gagctgcacc tggacgtgta catcgagcgc atgcgccgcg agtacaagtg cgaggtgacg
     1501 accggcatgc cgcaggtggc ctaccgcgag gcgatcagcc agcgggccga gttcaactac
     1561 acgcacaaga agcagaccgg cggctcgggc cagtacggcc gcgtcgcggg ctacatggag
     1621 cctatagagg gcgcggtcta cgagttcgtc gactccgtca agggcggggc cataccgaac
     1681 gagtacatac cgtcctgcga caagggcttc aaggaggcgg ctaagaagg
//
LOCUS       DAOPHV010000594         1725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_99692_length_1725_cov_3.777246, whole genome shotgun sequence.
ACCESSION   DAOPHV010000594 DAOPHV010000000
VERSION     DAOPHV010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99692_length_1725_cov_3.777246"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..>1725)
                     /locus_tag="PLQ29_12440"
     CDS             complement(223..>1725)
                     /locus_tag="PLQ29_12440"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPG87497.1"
                     /translation="VDPAAQAMMTLSKAQKQTMDGYLALAPSDPSLKLDAFPEWEIVL
                     RELQADINAFYAKNKGKSAGKDMPGFDSVALHMWHIYTGGLRQMSDGSWIAPKLDLAR
                     KLASSSLDTFVWMDSVGLNASYGGKANYGGYPGLGTVLGAMWPRTHSFMSGADRIPQL
                     EKKALEFGVKIYTETRGTELVADASGRIVGAKAVQADGTSVSIKTAKGVVLATGGYCA
                     NPVMVKKYDKYWGDDLSERTLSTNMGTNTGDGIVMAMSVGAATTGLEVAQMMPSSSPT
                     KGTMTDGIWADAAEQIWIDGAGNRFVNEYAERDVLAKASLALDRGVFYIIYAGRGDPG
                     NPGMMIKGTTKDARIRDMVEGNHIWYGSTLAELAEASNKPAAGCTPRFTERQLRETIE
                     RYNAAVAAQKDPDFGKEVLAGAIDLAYIESHDDVGICISPRKASLHHTMGGVSIDTET
                     RVLGADGKPIMGLWAAGEVTGGIHAGNRLGGNAIADIFTFGRIAGVNAAK"
BASE COUNT          278 a          532 c          557 g          358 t
ORIGIN      
        1 ggagagagag tcctacccct gctaaaaaca aaggtcacgc gacagcgtgg cctttgtctt
       61 atggagtagg actcgaacca ttcgccgcga gccggcgagc ggcagagaag cgccagacgg
      121 atcctggtaa cgctcggaga gaaagaaggg gctgcccgat tttccgggca gccccttcgt
      181 ataatctcgg tagctgttta ctgtacgatc tgcctgcttg gtctacttgg ccgcgttgac
      241 gccggcgata cgcccgaagg tgaagatgtc ggcgatggcg ttgccgccga gtcggttgcc
      301 ggcatggatg cctcccgtga cctctcccgc ggcccagaga cccatgatgg gcttaccgtc
      361 ggcgcccagg acgcgcgtct cggtgtcgat gctcacgccg cccatggtgt ggtgcagcga
      421 agccttgcgg gggctgatgc agatgcccac atcgtcgtga gactcgatat acgccaggtc
      481 gatggcgccg gccagcactt ccttgccgaa atcaggatcc ttctgggcgg cgacggccgc
      541 gttgtaccgc tcgatggtct cccgtagctg acgttccgtg aagcgcggtg tacagcccgc
      601 cgcgggcttg ttgctggcct cggccagctc ggcgagggtg gagccgtacc agatatggtt
      661 tccttcgacc atatctctga tacgagcgtc tttcgtcgtg cccttgatca tcatgcctgg
      721 attgcccgga tcgccacgtc cggcgtagat gatatagaag acgccgcggt ccagggcgag
      781 tgacgccttg gcgaggacgt cacgctccgc gtattcgttg acgaagcgat tgcccgcccc
      841 gtctatccag atctgctcgg ccgcgtcggc ccagataccg tcggtcatcg tgcccttggt
      901 cggggaggac gagggcatca tctgggccac ctcgaggccg gtcgtggccg cgcctacgct
      961 catagccatc acgatgccgt cgccggtgtt ggtgcccatg ttcgtcgaca gagtgcgctc
     1021 agacaggtcg tctccccagt acttgtcgta cttcttgacc atgaccgggt tggcgcagta
     1081 gccgccggtc gccagcacca cgcccttggc cgttttaatc gacacggacg tgccgtccgc
     1141 ctgcacggcc ttggcgccga cgatcctgcc ggaagcgtcg gctacgagct cggtgccgcg
     1201 ggtctcggta tagatcttca cgccgaattc gagcgccttc ttttcgagct ggggtatgcg
     1261 gtcggcaccg gacatgaagc tgtgtgtgcg cggccacatg gcgccgagaa ccgttccgag
     1321 gccggggtag ccgccgtagt tggctttacc accgtatgag gcgttcagcc cgacgctgtc
     1381 catccagacg aatgtgtcga gtgagctgga ggccagtttc ctcgctaggt cgagcttggg
     1441 cgctatccac gagccgtcgc tcatctgcct gagaccaccc gtatagatat gccacatgtg
     1501 cagggcgact gagtcgaagc cgggcatgtc cttgccggcc gacttgccct tgttcttggc
     1561 gtagaaggcg ttgatatcag cctggagctc gcgtagcacg atttcccatt cggggaacgc
     1621 gtcgagcttc aggctagggt cggagggggc gagggccagg tagccgtcca tcgtctgctt
     1681 ctgcgccttg ctcagcgtca tcatggcctg cgcggccggg tcgac
//
LOCUS       DAOPHV010000595         1725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_99701_length_1725_cov_3.443114, whole genome shotgun sequence.
ACCESSION   DAOPHV010000595 DAOPHV010000000
VERSION     DAOPHV010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99701_length_1725_cov_3.443114"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLQ29_12445"
     CDS             <1..206
                     /locus_tag="PLQ29_12445"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPG87498.1"
                     /translation="LDEAFPGCRAGERPDGSAVFAFKYPEGAWLVRFLLSVGPGLVVR
                     KPESVRAAVAKAAMEIAEANEAP"
     gene            265..603
                     /locus_tag="PLQ29_12450"
     CDS             265..603
                     /locus_tag="PLQ29_12450"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HPG87499.1"
                     /translation="MDTMIAYCGIDCAACDARKATLTNDDALRAETAAKWNAAFGFPE
                     NPALVDCVGCSGDGAKIGHCAECDMRLCAMAKGLATCAGCAEFEACERIRRFLADVPE
                     ARANLALLRG"
     gene            complement(633..>1725)
                     /gene="glgP"
                     /locus_tag="PLQ29_12455"
     CDS             complement(633..>1725)
                     /gene="glgP"
                     /locus_tag="PLQ29_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPG87500.1"
                     /translation="ARADDALSPSALTISFARRFATYKRGNLLLTDPDRLLRLLSDKD
                     RPLQIIFAGKAHPHDMPGKEIIKELVHFSRRDDVAGRIVFLEDYDMTMARYMTSGSDV
                     WLNTPRRPLEASGTSGMKAGMNGVLNCSILDGWWAEGYTPECGWAIGKGEEYVDTELQ
                     DEVESKALYDLLEREIIPTFYERGRDGLPRDWIRMMKCSIRDVGKNFSFHRTLMEYTE
                     RYYLPALENARRLRSEGFGKAKETAEYLELARREWQHVAVEDVRSTARPVMERGDAVR
                     VEARVRTGALKPEQVLVELYHGPVSSTGAIYEPARVEMKASASQGGAYEYSVEVACES
                     TGQHGYSVRVLPRHEGLAHPFIPGLVRWA"
BASE COUNT          258 a          581 c          597 g          289 t
ORIGIN      
        1 ccctggacga ggcgttcccc ggttgccgcg cgggcgagcg ccccgacggc tccgccgtct
       61 tcgccttcaa gtaccctgaa ggagcctggc tcgtacgctt cctcctgtcg gtcggccccg
      121 gcctcgtcgt acggaagccc gagagcgtgc gcgccgccgt agcgaaggcc gcaatggaaa
      181 tcgccgaggc gaacgaggct ccctgacgct aattgtcagg gacggcgcgc tagaatcgtc
      241 ccatcacgtt tcgaggagac gaatatggac acgatgatag cctattgcgg gatcgactgc
      301 gccgcgtgcg acgcgcgcaa ggccacgttg acgaacgacg acgcgctgag ggccgagacg
      361 gccgccaagt ggaacgccgc gttcggtttc ccggagaacc cggccctggt cgattgcgtc
      421 ggctgctccg gcgacggcgc caagataggc cattgcgccg aatgcgatat gcgcctgtgc
      481 gcgatggcca aggggctcgc gacctgcgcc gggtgcgccg agttcgaggc ctgcgagcgc
      541 atccgccggt tcttggccga tgtgcccgag gctagggcga acctggcgct gcttcgcggg
      601 taggcgggtc ccgcccgcga agcctaccgg actcaggccc agcgtaccaa gcccgggatg
      661 aacgggtgcg cgagaccctc gtgcctgggt agcacgcgga cggagtagcc gtgctggccg
      721 gtcgactcgc aggcgacctc caccgagtac tcataggcgc cgccctgcga ggcgctggcc
      781 ttcatctcga ccctggcggg ttcgtagatc gctcctgtgc tcgatacggg gccgtggtac
      841 agctcgacga ggacctgctc cggcttgagc gcgcccgtgc gcacgcgcgc ctcgacgcgc
      901 accgcgtcgc cgcgctccat caccggcctg gccgtcgacc tgacgtcctc gacggccacg
      961 tgctgccact cgcgcctggc cagctccagg tactccgcgg tctccttggc cttaccgaag
     1021 ccctcggagc ggagcctgcg ggcgttctcg agcgccggta ggtagtagcg ctcggtatac
     1081 tccatcagcg tgcggtggaa cgagaagttc ttgccgacgt cgcggatgga gcacttcatc
     1141 atcctgatcc agtcgcgggg caggccgtcg cggccgcgct cgtagaaggt cgggatgatc
     1201 tctcgctcca gcaggtcgta gagcgccttg gactcgacct cgtcctgcag ctcggtgtcg
     1261 acgtactcct cgcccttgcc gatggcccag ccgcactcgg gagtatagcc ctcggcccac
     1321 cagccgtcga gtatcgagca gttgagcacc ccgttcatgc cggccttcat cccgctcgtg
     1381 ccggaggcct cgagaggccg gcgcggggtg ttgagccaga cgtccgagcc cgaggtcatg
     1441 tagcgggcca tcgtcatgtc gtagtcctcg aggaacacga tgcggcccgc cacgtcgtcg
     1501 cggcgcgaga agtgcaccag ctccttgatg atctccttgc ccggcatgtc gtgcgggtgg
     1561 gccttgcctg cgaagatgat ctgcagcggc cggtccttgt ccgacaggag cctgagcagg
     1621 cggtcggggt cggtcaggag caggttgccg cgcttatagg tggcgaagcg acgggcgaag
     1681 ctgatcgtga gcgcggaggg ggacagcgcg tcgtcggccc tggcc
//
LOCUS       DAOPHV010000596         1724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_99812_length_1724_cov_2.644098, whole genome shotgun sequence.
ACCESSION   DAOPHV010000596 DAOPHV010000000
VERSION     DAOPHV010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99812_length_1724_cov_2.644098"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1701)
                     /locus_tag="PLQ29_12460"
     CDS             complement(31..1701)
                     /locus_tag="PLQ29_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG87501.1"
                     /translation="MANDKHIVVIGGVAAGATAAARAKRLDPTAKVTIIEGGRYVSFA
                     NCGLPYFVSRDIDKRSALILQTPEGFMARYGVEVLLETRATAIDRAARTIAVDGPAGK
                     AEVAYDSLILAQGGLPFMPPIEGSDSDTVFRLWTIPDMDAVHSFIEKRKPASALVVGG
                     GFIGLEAAEAFVARGLATTVVELTSHVMPPADAEFGAMIGRAFEEAGARVITGRSVKA
                     IRGGVALLDDGTEVPAELVLVSAGVKPNVELARSAGLELGKTGALVVDEYMRTSDGHI
                     WAAGDMVEIRSRVSGQATRVPLAGPANRQGRIAATNALGGSMRYAGASGTSVFKAVDK
                     TFAMTGLSERAAKGLGLDAASVTVHRYHHVSYYPGAEELSLKLVYERGSGRLLGAQAF
                     GKEGVDKRIDVAATALAGGMTIDDLAELDLAYAPPYGAANDPVNMAAFAAQNAESGYA
                     PTVSAEEAWALAMSGEASIVDLRTNGDRRKAPVPSAVQIPLDELDWRLEELPAGRLLL
                     LSRAGYESHIALRKLLGLGRTDAQNISGGELSLELVPGFVQVLESIER"
BASE COUNT          262 a          581 c          581 g          300 t
ORIGIN      
        1 ccaacatggg tacgcctcga aatcgggcgc ttatcgttcg atcgactcga gcacctggac
       61 gaagccgggg acgagttcca ggctcagctc gccgcccgag atgttctgag cgtcggtcct
      121 gcctaggccg aggagtttgc gtagggcgat gtggctctcg tagccggccc tggacaggag
      181 gaggagtcta cctgcgggca gctcctcgag cctccaatcg agttcgtcga gggggatttg
      241 gactgccgac gggaccggcg ccttgcggcg gtcgccgttg gtccgcagat cgacgatgga
      301 cgcctcgccc gacatagcga gcgcccaggc ctcctcagcc gataccgtcg gagcatagcc
      361 ggactcggcg ttctgggcgg cgaaggcggc catgttgacg gggtcgttag cggccccgta
      421 gggcggcgcg taggccaggt caagttcggc taggtcgtca atcgtcatgc cgccggcgag
      481 cgccgttgcg gccacgtcga tgcgcttgtc gacgccctcc ttaccgaagg cctgggcccc
      541 gagcaagcgg cccgagccgc gctcgtagac cagcttgagg ctcagctcct cggcgcccgg
      601 gtagtagctc acatggtggt agcgatggac cgtcaccgag gcggcgtcca ggccgaggcc
      661 cttggccgcg cgctcggaga ggccggtcat cgcgaaggtc ttgtccacgg ccttgaagac
      721 gctcgtgccc gaggccccgg cgtagcgcat cgagccgccg agcgcgttcg tcgcggctat
      781 gcggccctgc ctattcgccg ggccggcgag cggcacgcgc gtcgcctgcc ccgagactcg
      841 gctcctgatc tcgaccatgt cgccggcggc ccagatatga ccgtcgctcg tcctcatgta
      901 ctcgtccacg acgagggcgc cagtcttgcc tagctcgagg ccggccgacc tggccagttc
      961 gacgttcggc ttgacgccgg cggacacgag cacgagctcc gcgggaactt ccgtgccgtc
     1021 gtcgagcagg gctacgcctc cgcgtatcgc cttgaccgag cggccggtga tcaccctggc
     1081 cccggcctct tcgaaagctc ggcctatcat cgccccgaat tcggcgtctg ccgggggcat
     1141 cacgtgcgag gtcagctcga cgacggtggt cgccagaccc cgggcgacga aggcctcggc
     1201 ggcctcgagg ccaatgaagc cgccgccgac gacgagagcc gacgctggct tgcgtttctc
     1261 gatgaacgag tgcacggcgt ccatgtcagg aatggtccag agtctgaaca cggtatcgga
     1321 atcggagccc tctatcggcg gcatgaacgg gaggccgccc tgggcgagta tcagcgagtc
     1381 gtaggcgacc tcggccttgc cggccggtcc gtcgacggcg atggtcctgg ccgccctgtc
     1441 gatcgcggta gcccgcgtct cgagcagcac ctcgacgccg taccgcgcca tgaagccttc
     1501 gggggtctgt aggatcagcg cgctgcgctt atcgatgtcc ctggagacga aatagggtag
     1561 gccgcagtta gcgaacgata cgtagcggcc accctcgatg atcgtgacct tagccgtagg
     1621 atcgagcctc ttggccctgg ccgccgcggt cgccccggcg gccactcccc cgattaccac
     1681 tatatgttta tcattcgcca tatcgtcctc ctcgttgttg gtcg
//
LOCUS       DAOPHV010000597         1715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_100615_length_1715_cov_3.199398, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000597 DAOPHV010000000
VERSION     DAOPHV010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100615_length_1715_cov_3.199398"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PLQ29_12465"
     CDS             complement(<1..573)
                     /locus_tag="PLQ29_12465"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HPG87502.1"
                     /translation="MMIMKEYGADGLEAMRQATIDRLTEAFAADVIGMDEYERRAEAA
                     NAAKRTDDLRALSADLPASRPAGPAVRGKATSKPASSSRKALDPSIVGAAPITTGCVM
                     GDRRLVGDWLSSDRVSAFTVMGSTKLDLRESVLPPGPIKIEAFTLMGEIQVIVPRDLP
                     VKLNAFAFMGESNAGKEVNQQVRGAETWVEV"
     gene            780..1166
                     /locus_tag="PLQ29_12470"
     CDS             780..1166
                     /locus_tag="PLQ29_12470"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HPG87503.1"
                     /translation="MEKVLIRLRMGAHDAHYGGELVDGAKMLQLFGDVATELLIRHDG
                     DEGLFAGYEKVEFLAPVFAGDYIEATGEIIHVGNSSRKMTFEARKVIAPRKDLSDSAA
                     DVLAEPVVVCRATGTCVVPKDKQRGA"
     gene            1271..>1715
                     /locus_tag="PLQ29_12475"
     CDS             1271..>1715
                     /locus_tag="PLQ29_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPG87504.1"
                     /translation="MPYSYFTIETADRVAVVTIRRPEALNALSTAVLRELDAVIGELE
                     SLAEADAVILTGEGKAFVAGADVAEMRGLSAEQGRAFGEYGSRVFRRLELLGKPVIAA
                     VNGFALGGGCELAMACDIRVASDKARFGQPEVGLGITPGFSGTQRL"
BASE COUNT          297 a          539 c          564 g          315 t
ORIGIN      
        1 cacctcgacc caggtctcgg ctccgcgtac ctgctggttg acttccttgc cggcgttgct
       61 ctctcccatg aacgcgaagg cgttgagctt gaccggcagg tccctcggca cgatcacctg
      121 gatctcgccc atcagcgtga acgcctcgat cttgatcggc ccggggggta gcaccgactc
      181 ccgtaggtcg agcttggtgg agcccatcac cgtgaaggcg gatacgcggt cgctcgacag
      241 ccaatcgccg accaagcgac gatcacccat cacgcagccg gtggtaatgg gcgcggcgcc
      301 gacgatagac gggtcgagcg ccttgcggga cgaggatgcc ggcttggacg tcgccttgcc
      361 cctgacagcc ggcccggccg gccgtgacgc cggaaggtcg gcagaaaggg cgcgcaggtc
      421 gtcggtccgc ttggccgcgt tggccgcctc ggccctgcgc tcgtattcgt ccatgccgat
      481 cacgtcggcc gcgaaggcct cggtcagcct gtctatcgta gcctgccgca tcgcctcgag
      541 accgtccgcc ccgtattctt tcatgatcat catcgtccgt tcctccgtcg cgggcttcga
      601 ataggaccct ttcggcgtcc ggaccctcta cccgtaaggc gtaggggcgt cgatccctgc
      661 gaatataata tcgcaaaata tggcgggctt ggctatggcc aagcccgggc ccgttcgctt
      721 ttcctttgcc gcaaacgggg ctatactaga tcctgtctgc taaaagggag gactctggta
      781 tggaaaaggt attgatcagg ctccggatgg gcgcccacga cgcgcactac ggcggagagc
      841 tcgtggatgg ggcgaagatg ctccagctgt tcggggatgt tgctacggag ctgctcatcc
      901 gccacgacgg cgacgaggga ctgttcgccg gctacgagaa ggtcgagttc ctggcgcccg
      961 tgttcgccgg cgactatatc gaggccaccg gcgaaatcat ccacgtgggc aactcgtcgc
     1021 gcaagatgac cttcgaggcc cggaaggtga tagccccgag gaaggacctc agcgactccg
     1081 ccgccgacgt gctcgccgag cccgtggtcg tctgcagggc gacgggtacc tgcgtggtac
     1141 cgaaggataa gcagcgcggg gcatgagcct ctggcgtcgt cgatatatgt taaaaaatat
     1201 cgatactaaa tgcttgacaa agcctcgggt gcggtctaca ttttctggtg ccggctaaga
     1261 ggaggcttcg atgccgtata gctactttac catagagacg gccgatcgcg tcgccgtcgt
     1321 aacgatacgt cggccggaag cgctgaacgc cttgtcgacc gccgtgctcc gggaactgga
     1381 tgccgtgatc ggcgaactgg aaagcctggc cgaggccgac gcggtgatct tgaccgggga
     1441 gggcaaggcc ttcgtcgccg gggcggacgt agcggagatg cgcggcctat cggccgagca
     1501 gggccgggcc ttcggagagt acggctcccg cgtattcagg cgcctcgagc tgctcggcaa
     1561 accggtcata gcggccgtca acggcttcgc gctcggcgga ggctgcgagc tcgcgatggc
     1621 ctgcgatatc cgcgtggcca gcgacaaggc caggttcggg caacccgagg tcggactcgg
     1681 catcacgccg ggtttctccg gtacccaacg cctgc
//
LOCUS       DAOPHV010000598         1714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_100682_length_1714_cov_4.088608, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000598 DAOPHV010000000
VERSION     DAOPHV010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100682_length_1714_cov_4.088608"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLQ29_12480"
     CDS             <1..427
                     /locus_tag="PLQ29_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87505.1"
                     /translation="GDPGGYGYVSHLDYLGYPYGGDVQLARADLEYLAPGVGTFSAYA
                     TAMIRGRMSFFTSHNLGGDNGGMANYEGSTPSGDAVDERLVVGLYARVALPKPAIAAE
                     ASAWLRVDWIGRRVRAKADGSYSDAEADLQVSAGLSASL"
     gene            complement(456..1283)
                     /locus_tag="PLQ29_12485"
     CDS             complement(456..1283)
                     /locus_tag="PLQ29_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPG87506.1"
                     /translation="MIDLHTHSTASDGALSPAALIGKAAEEGLGAIALTDHDCLDGLD
                     EARTAASSIGMRFIAGIEIEIAFEPGEFHLLGLDLFDTRRPLRDAAASLADSRERRNR
                     AVLELFRDDGVELDYDELRAKAGTGMIGRPHIASMLVEKRIVRSKQAAFDKYLAKGRP
                     YYVRKACLELPEALRVVKDSGGLAFVAHPMSLFASWKRLRALFEEWKALGVDGIEAWH
                     PTARLVDCQRLDAMAREFGFRVSAGSDFHGVARPDRRLGHTAGKRRIEDEFLACLER"
     gene            1373..>1714
                     /locus_tag="PLQ29_12490"
     CDS             1373..>1714
                     /locus_tag="PLQ29_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87507.1"
                     /translation="MKTEGDADAPQPWQAPIRSKFRRKYPDDRRYTDDRRYPDDRRYT
                     DDRRYPDDHPWFALDHAAIIYPSIMSDRVSAYYRIQAILDEPVDIALVQRALDALRPR
                     FPYFQIELRRGF"
BASE COUNT          288 a          578 c          574 g          274 t
ORIGIN      
        1 cggcgacccg gggggctacg gctacgtgag ccacctcgac tacctgggct atccgtacgg
       61 cggcgatgtc cagctggcgc gagccgacct cgagtacctg gcgccgggcg tcgggacctt
      121 cagcgcctac gcgacggcca tgatacgcgg ccggatgagc ttcttcacct cgcacaacct
      181 gggcggcgac aacggcggaa tggcgaacta cgaaggctcg acgccgtcag gcgacgcggt
      241 cgacgagcgc ctcgtcgtcg gtttgtacgc tagggtagcg ctaccgaagc cggccatcgc
      301 cgccgaggct agcgcctggc tacgggtgga ttggataggc aggcgcgtcc gcgccaaggc
      361 cgacgggtcg tatagcgacg cggaagccga ccttcaggtg tcggccgggc tgtcggcgtc
      421 gctctagtcg cgccgaggcg actgcgacga gacgcctagc gctcgaggca ggccaggaac
      481 tcgtcctcga tgcggcgctt gccggcggtg tggccgagac gtcggtccgg acgggcgacg
      541 ccgtgaaaat cgctgccggc gctgacgcgg aagccgaact cgcgggccat cgcgtcgagg
      601 cgctggcagt cgacgaggcg ggcggttggg tgccaggcct cgatgccgtc gacgccgagg
      661 gccttccatt cctcgaacag ggcgcgcagg cgcttccagc tcgcgaacag ggacattgga
      721 tgggcgacga aggccaagcc tcccgagtcc ttgacgacgc gtagcgcctc gggcagctcg
      781 aggcaggcct tgcgcacgta gtaaggccgc cccttggcca ggtacttgtc gaaggcggcc
      841 tgcttgctac gcacgatgcg cttctccacc agcatcgagg ctatatgggg acggccaatc
      901 atgccggtgc cggccttggc gcgtagctcg tcgtagtcga gctcgacgcc gtcgtcgcga
      961 aacagctcca ggaccgcccg gttacgccgt tcgcgcgagt ccgccagcga ggcggccgcg
     1021 tcgcgcaggg gccgacgtgt atcgaacagg tccaggccga gcaggtggaa ctcgccgggc
     1081 tcgaacgcta tctctatctc gatgcccgct atgaaacgca tgccgatgga cgaggcggcg
     1141 gtccgcgcct cgtccaagcc gtccaggcag tcgtgatcgg tcagcgcgat ggcgccgagg
     1201 ccttcctcgg cggccttgcc gatgagggcc gccggggata gggcgccgtc gctcgccgta
     1261 gaatgcgtat gcagatctat catctgatcc tattctgccg ctttcatcgt tttttgcata
     1321 gcgcccgcgc tcggcatggg caaagcgacg acttgtcagt attataatag ccgtgaaaac
     1381 cgaaggcgac gcggatgcgc cgcaaccatg gcaggctccc attcgctcca agttccgccg
     1441 taagtacccc gacgaccggc ggtacaccga cgaccggcgg taccccgacg accggcggta
     1501 caccgacgac cggcggtacc ccgacgacca tccctggttc gcgctcgatc acgcggccat
     1561 catctacccg tccatcatgt ccgaccgcgt cagcgcctac taccgcatcc aggccatact
     1621 cgacgagcca gtagacatcg ccctcgtcca gcgagcgctg gacgcgctca ggccgcggtt
     1681 cccctatttc cagatcgagc tgaggcgagg cttc
//
LOCUS       DAOPHV010000599         1711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_100920_length_1711_cov_4.664251, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000599 DAOPHV010000000
VERSION     DAOPHV010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100920_length_1711_cov_4.664251"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            584..1633
                     /locus_tag="PLQ29_12495"
     CDS             584..1633
                     /locus_tag="PLQ29_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87508.1"
                     /translation="MMAAHDDLDLRMLRIDKVLWSSGIRSSDVEALKKMQSSGKTNGT
                     NGSASTPGDSGDPMDPSAGGGTGSAIGEGTGTSGSGEATGGSLGGKPEGSAQISGEIR
                     LQGVLRYILPQISGGFKAPSCKVSWIIHWWHPPIPKFEFSRGYARVQVESAQQFDFKL
                     TGTLNLSAEVKVPIAMFVVIEPHTQLKFECGLYAKITLDGSITLGFEVSEYSRSEAHA
                     GCDLVWPFIPVKVGGGQDNYFHTAFRPFLAASAELTGGLYVGGELSWSGIGLASLEAG
                     GGLYVGVNGYIEPLGVMGYDSKIGGYGSFDEWIIYVNAEAGAFFKVTLGLLIWSHDLF
                     SMRWPFWTSANQWEF"
BASE COUNT          307 a          559 c          565 g          280 t
ORIGIN      
        1 ccttcttcgg ctacgacgag gtgggtaccc tgcgcggctc ggtggtccgc tcggtcggca
       61 ccgactcggt ggtcatgaag gactacgatg agtagccgcg aaaaagccct cctcgtcgcc
      121 ttggcgatca tcgtcgtcgg cgtcgcgacg gtcacggccg atccggctca gttcagcgga
      181 gcggagctcg tcttcatgcc ggccgtctct gacatgcgcg tggtcggcgc gatcatggcc
      241 cgcagcgcca acgcgccgac gccggccaag agcctcaagg acctgcgggc cggtagcgtc
      301 ttcatcaacg aggacggcca ggcccgcatc gtcaccgcgg tctacgagga ccgcggccgc
      361 gtcgtcatcg agaccgagga acccaggccg gagtacgtga tcagctatgc ctacgtgccg
      421 gatttccagc tgcagatgac gatggacaac ctcgcgagcc tcgcccccgg cgtgtcgata
      481 gccgagccgg gccgacgacg cgcgcgatct gttcgagagc gacctggacg ccgccacccg
      541 cgacaaggtg tcctggatca ccaccgacga cgagatcgac gacatgatgg ccgcccacga
      601 cgacctcgac ctccggatgc tcaggataga caaggtgctc tggtcgagcg gcatccggtc
      661 ctccgacgtc gaggcgctca agaagatgca gtcctcgggc aagacaaacg gcacgaacgg
      721 ctcggcctcg acgccgggcg attcgggcga tccgatggac ccatccgccg gcggcggcac
      781 gggtagcgcg ataggcgagg gtaccggcac ttccggctcg ggagaggcca cgggcggttc
      841 cctcgggggc aagcccgagg gctcggccca gatcagcgga gagatccgcc tccagggcgt
      901 gctacgctac atcctgcccc agatctccgg cggcttcaag gcgccctcat gcaaggtgtc
      961 gtggatcatc cactggtggc acccgcccat acccaagttc gagttcagcc ggggctacgc
     1021 gagggtgcag gtcgagtcgg cccagcagtt cgacttcaag ctgaccggca cgctcaacct
     1081 gagcgccgag gtcaaggtgc cgatagcgat gttcgtcgtg atcgagccgc atacccagct
     1141 caagttcgag tgcggactct acgccaagat caccctggac ggcagcatca ccctgggctt
     1201 cgaggtgtcc gagtactcga ggagcgaagc ccacgccggt tgcgatctcg tctggccgtt
     1261 cataccggtc aaggtcggag gcggccagga caactacttc cacacggcgt tcaggccgtt
     1321 cctggccgcg tccgcggagc tgaccggcgg cctgtacgtt ggcggcgaac tgagctggtc
     1381 gggcataggg ctggccagcc tcgaggccgg cggcggactc tacgtgggcg tgaacggcta
     1441 catcgagccg ctcggcgtga tgggctacga ctccaagata ggtggctacg gctccttcga
     1501 cgaatggatc atctacgtca acgccgaggc gggagccttc ttcaaggtga ccctgggatt
     1561 gctgatctgg agccacgacc tcttcagcat gcgatggccg ttctggacct cggccaacca
     1621 atgggagttc tagcgatggc cgatacgcgt ataacgaaga accgggtagc gctagccctg
     1681 atagcggcgg cgctcattgc ctcggccgtc t
//
LOCUS       DAOPHV010000600         1711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_100953_length_1711_cov_3.084541, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000600 DAOPHV010000000
VERSION     DAOPHV010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100953_length_1711_cov_3.084541"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="PLQ29_12500"
     CDS             complement(<1..864)
                     /locus_tag="PLQ29_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87509.1"
                     /translation="MAVLVKNIEREFLLSTAMRERSPITMGAGGGEWTVCILAVEKGK
                     LSFGHELPLDLQRKGSAYDFRYNVRGQTIAFKATMLEPGERRFATSMPDKLYKNLSRR
                     FVRLPPPGDLSASFAFAGERYDLDFPSSAGSGPSREPEPSPDFDPSDLRGLMAEFERK
                     ALAVASDRGIVMFKDRKPETVPERLAATTGRCFYLPTALGGLPRSDPFAERTILTRDD
                     FKQHFIDSGLEEDFAEDEIVRLERSKRSSNVLSELIVPILFQDYAIGYATVVNRQAGK
                     PPFDLRTVETFL"
     gene            complement(926..>1711)
                     /locus_tag="PLQ29_12505"
     CDS             complement(926..>1711)
                     /locus_tag="PLQ29_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87510.1"
                     /translation="RSRLYAITSAAIMLAVAVSALACSKSKETPAVETARTPEPASSP
                     AAATRDDAARDDAARDDAARDEPSQKPADKPVAMKASPKASTSVETDAKASKAIVSSN
                     LAGVDRRLVERPFDALAAGDFELGELYGSGASAPANAALSVALLELERSLLEGEPPYG
                     SMGAEASALARARFGDAFPLSFARVRFARPVVGPALVASVPFRAFADDSGARAIIGLA
                     ILAADDDGTWVIEHLELDPEALEDGASRSEPWDPYDTSRQAYE"
BASE COUNT          268 a          547 c          600 g          296 t
ORIGIN      
        1 caggaaggtc tcgacggtgc gtaggtcgaa cggcggcttg cccgcctgcc tgttgactac
       61 cgtggcgtag ccgatggcgt agtcctggaa caggatgggc acgatgagct cggacagcac
      121 gttcgacgag cgcttcgagc gctccagcct gacgatctcg tcctccgcga agtcctcctc
      181 gaggcccgag tcgataaaat gctgcttgaa gtcgtcgcgg gtcaggatgg tccgctcggc
      241 gaaagggtcg ctgcgtggca ggccgccgag cgccgtcggt aggtagaagc aacggccggt
      301 ggtggccgcc agccgctccg ggacggtctc cggcttgcgg tccttgaaca tcacgatgcc
      361 gcggtcgctg gcgacggcga gggccttgcg ctcaaattca gccatcaggc cgcgcaggtc
      421 ggacgggtcg aagtcgggcg acggctccgg ctcgcgggac gggcccgagc ccgcgctcga
      481 gggaaaatcc aggtcgtagc gctctccggc gaacgcgaag gaggcggaaa ggtcgcctgg
      541 cggaggcagg cgcacgaaac gcctgctcag gttcttgtag agtttatcgg gcatcgacgt
      601 agcgaagcgg cgttctcccg gctcgagcat cgtcgccttg aaggctatcg tctggcccct
      661 gacgttgtag cggaagtcgt aggcggagcc tttacgctgc aggtcgagcg gtagctcgtg
      721 gccgaagctc agcttgccct tctcgacggc caggatgcac acggtccact cgccgccgcc
      781 ggcgcccatc gtgatgggcg aacgctcgcg catcgccgtc gacaggagaa attctcgctc
      841 tatgtttttt actagcaccg ccatcggcat ggtcctccga tagcattgtg cctcaatcgc
      901 gatgattgtt caacgttttc gtcgattatt cgtaggcctg gcggctcgta tcgtaggggt
      961 cccatggctc cgaccgagac gctccgtcct cgagcgcctc cgggtcgagc tccaggtgct
     1021 cgatgaccca ggtcccgtcg tcgtcggcgg ccaggatggc caggccgatg atagcccggg
     1081 cgccgctatc gtcggcgaag gctcggaacg ggacgctggc gaccagcgcc ggtccgacga
     1141 cgggcctggc gaaacgtacc cgggcgaacg atagcgggaa cgcgtcgccg aacctggcgc
     1201 gggccagggc cgacgcctcg gcgcccatcg atccgtacgg cggctcgccc tcaaggaggc
     1261 ttcgctccag ctcgagaagc gccacgctca gcgcggcgtt agccggggcg ctcgcgcccg
     1321 agccgtagag ctcgccgagc tcgaagtccc cggcagctag cgcgtcgaaa ggccgttcga
     1381 cgaggcgccg gtcgactccg gctaggttcg acgagacgat cgccttggag gctttcgcgt
     1441 ccgtctcgac ggaggtcgag gccttgggcg acgccttcat cgcgacgggt ttatcggcgg
     1501 gcttctggct aggctcatcg cgcgcggcgt catcgcgcgc ggcgtcatcg cgcgcggcgt
     1561 catcgcgcgt agcggcggcc ggcgacgatg cgggctcggg cgtacgagcc gtctcgaccg
     1621 ccggcgtttc cttgctcttc gagcaggcta aggccgagac ggctactgca agcatgatag
     1681 cggcgctcgt aatggcgtat agtcgagatc g
//
LOCUS       DAOPHV010000601         1705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_101457_length_1705_cov_4.092121, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000601 DAOPHV010000000
VERSION     DAOPHV010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101457_length_1705_cov_4.092121"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLQ29_12510"
     CDS             complement(<1..213)
                     /locus_tag="PLQ29_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPG87511.1"
                     /translation="MTTEIRNLGYYAGGRQKISATTTYMDCFDPSTGEVIARAPQCLP
                     AEVEEAVAAAAAAYPAWSSTPVTKRVQ"
     gene            complement(288..>1705)
                     /locus_tag="PLQ29_12515"
     CDS             complement(288..>1705)
                     /locus_tag="PLQ29_12515"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPG87512.1"
                     /translation="RAVLHGLYDLDRAAAERRAAEWGAPRVYASYAEVLADPEVDAVE
                     VLVPHPLHEELAVAALRAGKHVSVQKPMTTSLRSADRMLAAARASGRVLKVAENYALY
                     PPIVLARRLIDEGAIGEPMTVRIKMMSGGSGGWPVSDSTWAWRLREFAAGRGMNTFDH
                     GHHMWATAWALLGPFRKVNAWVDEQNGVVDSPAVVMWAHEGPRRYGQCEFHYGKELAV
                     PSSYYGNDEWIDVSGSRGVLVIRRCTASILEGPVVGVYSGGAWAWHEAETDWATGFRL
                     STKNFIAAMRGDEAPRPSAEEGRHLLAMDLAVAKSDRERRTVWLGELDSPAPRLYAAL
                     ARRSERRAKAAFFRVLAGGGDDVACGDGDRARELTLALPGRFRAEAASGFEGTVGLAL
                     SDCAGGFSLTVAGRTISVIETSVPVTVPDTAVLCLRVSSSTWASILEGRSRIETAYLR
                     GKLRVDGEIALALRLRDMLGL"
BASE COUNT          277 a          601 c          564 g          263 t
ORIGIN      
        1 ctggacgcgc ttcgtcaccg gggtcgacga ccaggcggga taggcggccg cggccgcggc
       61 gacggcctcc tccacctccg ctggcaggca ctgcggcgcc ctggctatga cctcgcccgt
      121 tgaagggtcg aagcagtcca tgtatgtcgt cgtcgcggat atcttttgcc gaccacccgc
      181 gtagtatccc aggtttctaa tttctgtagt catatcttct ccttccttgt aatcgttgaa
      241 gcgaagctct gtccccggaa gcgaagctct gtccccagga agcggcttta taaacccagc
      301 atgtccctga gcctcagggc cagggcaatc tccccgtcga cgcgcagctt gccacgcagg
      361 taggccgtct cgatgcgcga ccgcccctcg aggatggagg cccaggtgga gctcgagacc
      421 cgtaggcaga ggacggcggt atcgggaacc gtaacgggga cagaggtctc gataacgctt
      481 atggttctac cggcgaccgt gagcgagaag ccgcccgcgc aatcgctcag ggcgaggccg
      541 acagtgcctt caaacccgct tgccgcctcg gccctgaatc ggccgggcag ggccagggtc
      601 agctcgcggg cccggtcgcc gtcgccgcag gctacgtcgt cgccaccacc ggcgaggacc
      661 ctgaagaacg cggccttggc cctacgctcg gaacggcggg ccagcgcggc gtagagcctc
      721 ggcgccggcg agtcgagctc tccgagccat acggtccggc gctcgcggtc ggacttggcc
      781 acggccaggt ccatggccag aaggtgccgg ccttcctcgg ccgacgggcg aggcgcctca
      841 tcgccgcgca tggccgcgat aaagttcttg gtcgacagcc tgaacccggt cgcccaatcg
      901 gtctcggcct cgtgccaggc ccaggcgccg cccgagtaga cgccgacgac cggcccttcc
      961 aggatgctgg cggtgcagcg ccgtatcacg agcacgcccc gggagcccga gacgtctatc
     1021 cattcatcgt taccgtagta gctcgaaggc accgcgagct ccttgccgta atggaactcg
     1081 cactggccgt agcgcctggg cccttcgtgg gcccacatga ccacggcggg ggagtcgacc
     1141 acgccgttct gctcgtccac ccaggcgttc accttacgga aggggccgag caaggcccag
     1201 gccgtcgccc acatgtggtg accgtggtcg aaggtgttca tgccccggcc cgccgcgaat
     1261 tcacgcaggc gccaggccca ggtcgagtcc gagacgggcc agccgcccga gccgcccgac
     1321 atcatcttga tacggacggt catcggctcg ccgatggccc cctcgtcgat caagcgccta
     1381 gcgaggacaa tcggcgggta cagggcatag ttctcggcca ccttcagcac gcggcccgaa
     1441 gcgcgggccg cggcgagcat gcggtcggcg ctacgcaagc tcgtcgtcat gggcttctgt
     1501 accgagacgt gcttgccggc gcgcagggcg gccacggcca gctcctcgtg gagggggtgg
     1561 ggcacaagca cctccaccgc gtcgacctcg ggatcggcca ggacctcggc gtacgaggca
     1621 tagacccgcg gcgctcccca ctcggcggcc cgacgctccg cggccgcccg gtcgagatcg
     1681 tagaggccgt gaagaacggc cctcg
//
LOCUS       DAOPHV010000602         1704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_101561_length_1704_cov_3.293511, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000602 DAOPHV010000000
VERSION     DAOPHV010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101561_length_1704_cov_3.293511"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLQ29_12520"
     CDS             complement(<1..564)
                     /locus_tag="PLQ29_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPG87513.1"
                     /translation="MEYAPRHARAYKPRYCELDERGEATPAAMMALFEETAVSHCDDT
                     GWDVFRLRREGLGWILLEGGFSMLRYPRYGERFTVETWLSGARLFYGLRGFEVKGDDG
                     STMGRAWSLWTFYDLARRRPAPILGDILAAWRPDPAVSPSRRTPKAGGAEPASCVSIP
                     SPEAAIEVRPLDIDTNGHVNNVRYLEWA"
     gene            complement(843..>1704)
                     /gene="gguB"
                     /locus_tag="PLQ29_12525"
     CDS             complement(843..>1704)
                     /gene="gguB"
                     /locus_tag="PLQ29_12525"
                     /inference="COORDINATES: protein motif:HMM:NF040906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPG87514.1"
                     /translation="IGLAIGVWQGFWIAYVRLPAFIVTLAGMLTFRGLTTFMLKGLTI
                     GPFPEVFQQISSGFVPDFLGDEESLYNITAIAVGIVIAIIYALSQLRQRLRRKRNGYE
                     CSPAPMFAGKLAIVVSVIALAAFWLASYRGVPIVFIIIGALVVAYSLLTSSTVPGRYL
                     YAMGGNEKAAQLSGINTNKVLFLAYVNMGVLSAIAGIAVASRLNAASPLAGQNFELDA
                     IAACYIGGASASGGIGTIGGAIIGALVMGVLNNGMSLLGIGSDIQQFVKGMVLLAAVA
                     FDVLSKRKAR"
BASE COUNT          295 a          566 c          587 g          256 t
ORIGIN      
        1 ggcccattcc aggtagcgca cgttgttcac gtggccgttc gtgtcgatat cgagcggccg
       61 cacctcgatg gcagcttccg ggctcgggat ggagacgcac gaagccggct cagccccacc
      121 ggccttaggc gtccgccgcg acggcgagac cgccgggtcc ggcctccagg ctgccaggat
      181 gtcgcctagg atcggggccg gccgccggcg cgcgaggtcg tagaaggtcc acagcgacca
      241 agcccgcccc atcgtcgacc cgtcgtctcc cttaacctcg aagccacgca ggccgtagaa
      301 caacctggcg ccggacagcc aggtctcgac cgtgaagcgc tcgccgtagc gcgggtagcg
      361 gagcatagag aagccgccct cgagcaggat ccagccgaga ccctcgcggc gcaggcggaa
      421 cacgtcccag ccggtgtcgt cgcagtgcga caccgcggtc tcctcgaaca gcgccatcat
      481 cgcggccggc gtcgcctcgc cgcgctcgtc cagctcgcag tagcgcggct tgtaggcccg
      541 cgcgtgcctc ggtgcatact ccatgatgcc cagtacgacg cgcagagcga atcgaaagtt
      601 gcgatcgccg actcatcgcg gcgatcgccg gccgtcgttc gacccggctc gctgagctcg
      661 cgggtcaacg gcggcgcgtt gcggggcggc gccgttgacc ggacgcgact gagtgttgtc
      721 cggatgcgac tgagcatcac ccggacgcga ctgagtgttg tccggatgcg gccgagcatg
      781 actcggccgt cgttgacccg gcccgcgata gctcgtgggt caacggcggc gcgccgcggt
      841 gactatcgcg ccttgcgttt cgacagcacg tcgaaggcga cggccgcgag gagcaccatg
      901 cccttgacga actgctggat atcgctgccg atgccgagca gggacatgcc gttgttgagc
      961 acgcccatga ccagggcgcc gatgatcgcg ccgcctatgg tgccgatgcc gccgctcgcc
     1021 gaggcgccgc cgatgtagca ggcggcgatg gcgtccagct cgaagttctg accggccagc
     1081 ggcgaggcgg cgttcagcct ggaggccacg gcgatgcccg ctatcgccga cagcacgccc
     1141 atgttgacgt aggcgaggaa gagcaccttg ttggtgttga tgcccgacag ctgcgcggct
     1201 ttctcgttac cgcccatggc gtagagatag cggccgggga cggtgctcga agtcaggagg
     1261 gaataggcta cgacgagggc cccgatgatg atgaagacga tcggcacgcc gcggtacgag
     1321 gcgagccaga acgccgcgag cgctatcacg ctgacgacga tcgcgagctt gccggcgaac
     1381 atcggcgcgg gcgagcactc gtagccgttc cgcttgcggc gcaggcgttg cctgagctgc
     1441 gacagcgcgt agatgatcgc gatgacgatt ccgaccgcga tcgccgtaat attgtacagg
     1501 gactcctcgt cgcccaggaa gtccggcacg aaaccggagc tgatctgctg gaacacctcg
     1561 gggaacggcc ctatcgtcag gcccttgagc atgaacgtgg tcaggccgcg gaaggtgagc
     1621 atgcccgcca gggtgacgat gaaggccggc agccgcacgt acgctatcca gaagccctgc
     1681 cagacgccta tggccaggcc gatg
//
LOCUS       DAOPHV010000603         1704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_101578_length_1704_cov_2.958156, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000603 DAOPHV010000000
VERSION     DAOPHV010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101578_length_1704_cov_2.958156"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PLQ29_12530"
     CDS             complement(<1..952)
                     /locus_tag="PLQ29_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPG87515.1"
                     /translation="MSLRDDALKLHKDHAGKLEIRSTVPLRDKADLSLAYTPGVAEPC
                     LEIAARPDAVYEYCGKGNLVAVVSDGSAVLGLGDIGPAAAIPVMEGKAALFKAFGGVD
                     AFPICLDTKDVDELVDTIARLEPVFGGVNLEDISAPRCFEVEARLKERMAIPVFHDDQ
                     HGTAIVLLAGLLNALKLVGKPIGELVVAINGAGSAGIAIARMLLAAGAGDVILSDIRG
                     ILRDGDESGLNRAQAEIAALTNRDKRRGLLADAMRGSDVFIGVSRANLVTRDMVRAMA
                     RDPIVFAMANPVPEIDPDEALAAGARVVGTGRSDRPNQVNN"
     gene            complement(964..1515)
                     /locus_tag="PLQ29_12535"
     CDS             complement(964..1515)
                     /locus_tag="PLQ29_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HPG87516.1"
                     /translation="MIRLSLPLGDEEARSLRAGDRVLLSGVLLTGRDAAHKRLVELME
                     RGGALPVDLSGQTIYYVGPCPAAPGRVIGSAGPTTSGRMDVYTPRLLDAGLKGMIGKG
                     PRSRAVIDAIMRNGAVYFAAIGEAGALLARAIKEARVVAFGELGPEAIYRLRVEDFPV
                     TVAVDANGADLYSIGRNSYRRHA"
     gene            complement(1512..>1704)
                     /locus_tag="PLQ29_12540"
     CDS             complement(1512..>1704)
                     /locus_tag="PLQ29_12540"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPG87517.1"
                     /translation="SYIKHIPAKDIEEINALGIGPQGFGGTVTALDVRIDTFPAHLAS
                     LPVVVNLQCHAARHQEAVL"
BASE COUNT          247 a          569 c          582 g          306 t
ORIGIN      
        1 cgttgttcac ctggttgggc cggtcggagc gcccggtgcc gacgacgcgg gctccggccg
       61 ccagggcctc gtcggggtct atctcgggta cgggattggc catcgcgaac acgatcgggt
      121 cgcgagccat ggcgcggacc atgtcccggg tcaccaggtt ggctctggag acgcctatga
      181 acacgtcgct gccgcgcatc gcgtcggcca ggaggcctcg tcgcttatcc cggttcgtca
      241 gcgccgcgat ctcggcctgg gcccggttca gccccgactc gtcgccgtcg cggagtatgc
      301 cgcgtatgtc ggacaggata acgtcgcccg ccccggccgc cagcagcatc ctggctatcg
      361 ctatgcccgc cgagccggct ccgttgatgg ccacgacgag ctctccgatc ggcttgccta
      421 ccagcttgag cgcgttcagg agcccggcca ggaggacgat cgcagtgccg tgctggtcgt
      481 cgtggaacac cgggatggcc atgcgctcct tgagcctggc ctcgacctcg aagcagcgcg
      541 gggcggagat gtcctcgagg ttgacgccgc cgaagaccgg ctcgaggcgg gctatggtat
      601 cgaccaactc gtcgacgtcc ttggtgtcca ggcagatggg gaacgcgtcg acgccgccga
      661 aggccttgaa gagggcggcc ttgccctcca ttaccggtat cgcggcggcg gggcctatat
      721 cgccgaggcc gagcaccgcg ctgccgtccg acacgaccgc gaccaggttg cccttgccgc
      781 agtactcgta gacggcgtcc ggcctggcgg ctatctcgag gcagggctcg gcgacgccgg
      841 gcgtataggc caggctgagg tcggccttgt ccctcagggg caccgtgctg cgtatctcga
      901 gcttgccggc gtggtccttg tgcagtttaa gcgcgtcgtc gcgtaacgac attggtcttc
      961 ctttcaggca tggcgccgat acgagtttct tcctattgaa taaaggtcgg ccccgttcgc
     1021 gtcgacggcg accgttaccg ggaagtcttc gacgcgcagc ctgtagatag cctccggtcc
     1081 gagctcgccg aaggcgacga cccgggcctc cttgatcgcc ctggcgagta gggcgccggc
     1141 ctcgcctatg gcggcgaagt agaccgctcc gttccgcatt atcgcgtcta tcacggcccg
     1201 gctccgcggg cccttgccta tcatgccctt gagcccggca tcgaggagcc tcggagtata
     1261 gacgtccatg cgcccgctcg tcgtcgggcc ggccgagcct atgaccctgc cgggggctgc
     1321 cgggcacggt cctacgtagt agatggtctg gcccgacagg tcgaccggaa gcgcgcctcc
     1381 cctctccatg agctcgacga ggcgcttatg cgcggcgtcc ctgcccgtaa gaagaacgcc
     1441 tgacaggagc acgcgatcgc cggcccggag gcttctcgcc tcctcgtcgc caaggggcag
     1501 gctcagtcgg atcataggac ggcctcctgg tgacgggcgg cgtggcactg caggttgacg
     1561 acgaccggca ggctcgcgag gtgagccggg aaggtatcga tccgcacgtc gagggcggtg
     1621 accgtgcctc cgaagccctg gggcccgata ccgagggcgt tgatctcctc gatatccttt
     1681 gcggggatgt gcttgatgta gctc
//
LOCUS       DAOPHV010000604         1702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_101753_length_1702_cov_2.924712, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000604 DAOPHV010000000
VERSION     DAOPHV010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101753_length_1702_cov_2.924712"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PLQ29_12545"
     CDS             complement(<1..621)
                     /locus_tag="PLQ29_12545"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPG87518.1"
                     /translation="MPIEQPVTAKPWKRLDTLTRSFIAVLASYQLAQFAVFSAYPLPL
                     GFFGAFALPFALSSMGFHLLTFALLVVFRNDFVIEPAGRRLERVNLANGITLFRVSTL
                     PTVLFVILASRDYPVRFSLVALVAIVFATDFLDGFVSRRYNETTRVGRMMDSASDYAL
                     LFVISLVFYYFRIIPAWFLCLLVGRLAGQILMVLAVLAVKKRVTPRT"
     gene            701..1285
                     /gene="pgsA"
                     /locus_tag="PLQ29_12550"
     CDS             701..1285
                     /gene="pgsA"
                     /locus_tag="PLQ29_12550"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPG87519.1"
                     /translation="MNVADALTASRLFMAPLFFVVFIWGDSLGLPPGAAVAILWALFA
                     LIELSDLLDGWAARSMKTVSAFGKLFDPFADVFARLTYFVCFAFTGIMPLWIFLVIIY
                     REFCQLFLRQLLAERGVAMGARKGGKTKAVFYMISGAASLVLWSLRSLGALPGIHEGL
                     AVAVFVLYCVSAALSVGSFVDYLVQFKKISSAGS"
     gene            1289..>1702
                     /locus_tag="PLQ29_12555"
     CDS             1289..>1702
                     /locus_tag="PLQ29_12555"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HPG87520.1"
                     /translation="MRPVASLSAEEASSVRFVLFDIDDTITEDGRLLRESYDAIWSLY
                     EAGIAAVPVTGRPAGWCDLIARQWPVSGVVGENGAFAFYMEGDRLDRLYHEAAPDPAG
                     VRDRLATLGREALEAFPGLRIAKDQPYRVFDVALDF"
BASE COUNT          309 a          497 c          557 g          339 t
ORIGIN      
        1 cgtacgcggg gtgacgcgct tcttgacggc gagtaccgcg agcaccatca ggatctggcc
       61 cgcgagcctc ccgaccagca ggcacaggaa ccaggccggt atgatgcgaa agtaataaaa
      121 gacgaggctg atgacaaaca ggagcgcata gtcgctcgcc gagtccatca tccggccgac
      181 ccgggtcgtt tcattatagc gccgcgacac gaagccatcg aggaagtcgg tggcgaagac
      241 gatggcgacc agggcgacga gggagaagcg gactgggtag tcgcggctgg ccaggatcac
      301 gaatagcacc gtcggcaggg tgctgaccct gaacagggtg atgccgttgg ccaggttgac
      361 gcgctcgagc ctgcgtccag ccggctctat cacgaagtca ttcctgaaga cgacgagcag
      421 ggcgaaggtg agcaggtgga agcccatcga gcttaacgcg aatggtagcg cgaaggcgcc
      481 gaagaagccg agcggtaggg ggtaggccga gaagacagcg aactgcgcca actgatacga
      541 ggcgaggacg gcgatgaagc tacgggtgag cgtatcgagc cgtttccatg gcttagcggt
      601 aacaggctgt tcgatgggca tatgcgtcct tatcggccga tacgagccgg ggaaatgata
      661 caacttgaca ccagcggtgt caaatggaca tactttcagt atgaacgtcg cagatgccct
      721 taccgcgagc cggcttttta tggctccttt gttcttcgtc gtgttcatct ggggcgattc
      781 gctcgggctt cctccgggcg cggccgtcgc tatactctgg gctctgttcg cgctgatcga
      841 gctatccgat ctactcgatg gatgggccgc caggtcgatg aagaccgtca gcgccttcgg
      901 caagctgttc gatcccttcg ccgacgtgtt cgccaggctt acctatttcg tctgcttcgc
      961 gttcaccggc atcatgcccc tctggatttt cctcgtcatc atctataggg agttttgcca
     1021 gctgttcctc aggcagctac tcgccgagcg cggcgtggcg atgggcgcga gaaagggcgg
     1081 caagacgaaa gccgtcttct atatgatatc cggagcggcc tccctggtct tgtggagcct
     1141 tcggtcgctc ggcgccctgc ccgggatcca cgagggcctc gctgtcgcgg tgttcgtcct
     1201 gtactgcgtc tcggccgctc tttcagtcgg ttccttcgtc gactaccttg tgcagttcaa
     1261 gaagatcagc tcggccggta gctgagccat gaggcccgtc gcgtcgctct cggccgagga
     1321 agcctcctcg gtcaggttcg tactattcga tatcgacgac acgatcaccg aggacggccg
     1381 cctgctgcgc gagtcgtacg atgccatctg gtcgttgtac gaggcgggca tcgcggccgt
     1441 gccggtgacc ggccggcccg cgggctggtg cgacctgata gccagacagt ggccggtgtc
     1501 gggcgtcgtc ggtgagaacg gcgccttcgc gttctatatg gagggcgata ggctcgatcg
     1561 gctctaccat gaagcggctc ccgatccggc cggcgtgcgc gatcgcctag caaccctcgg
     1621 acgggaggcg ctcgaggcct ttccgggctt gcgtatagcc aaggatcagc cgtatcgcgt
     1681 gttcgacgtg gcgctcgact tc
//
LOCUS       DAOPHV010000605         1693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_102505_length_1693_cov_3.377289, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000605 DAOPHV010000000
VERSION     DAOPHV010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102505_length_1693_cov_3.377289"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1693)
                     /locus_tag="PLQ29_12560"
     CDS             complement(<1..>1693)
                     /locus_tag="PLQ29_12560"
                     /inference="COORDINATES: protein motif:HMM:NF015089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HPG87521.1"
                     /translation="GVAAQVYADYRRDRVFVVGRLEDGRSFAAEDASWRPVVYVAADE
                     AASPRARAVACRFAPCALEAIDGRGLLSWTAPSFGAYRDAVAALERASLAVHGSGPIA
                     ELYRADRGLALGVRVSGDERAGRRVGLVLADALVEADPDAAAPLSVLSLDIETDESDR
                     SVRAASLSMADWSPSGGGLVTRQGSTIVLVVAAPGAVGREALAAGQAQGYEAIGCPDE
                     ASLVRALSERVLALDPDVITGWNVVDFDLARLVERAEANGLALDIGRSSDGARVLPKG
                     RGRTATAVVPGRQVIDAMRVARSGPERYDDYSLETVARRALGQGKGSALRGRAKLEWL
                     DRAYRDDPSAFADYAALDAELVPQILDRTGLFALTVARAALVGVGLERAWTSVAAFER
                     AYGQALRDRSVAPPPFEQGRRVSGAAGGTVLPAEAGYRDGVVVLDFKSLYPSVMRTFC
                     VDPLAYERSGRPDDVVAPNGARFAREKPGERWPLPSLVDAYMARREAAMAEGDATGSY
                     VYKILMNSFYGVLGTDSCRYARTELAGAITSFGRLVLLRAKARIDILERENELKR"
BASE COUNT          247 a          620 c          627 g          199 t
ORIGIN      
        1 tcacgcttca gctcgttctc gcgttcgagg atgtcgatgc gcgccttggc ccgcaggagg
       61 acgagcctgc cgaacgaggt gatcgctccc gccagctcgg tacgggcgta gcggcaggag
      121 tcggtaccga ggacgccgta gaacgagttc atcaggatct tgtagacgta ggagccggtc
      181 gcgtcgcctt cggccatcgc cgcctcgcgc ctggccatgt acgcgtcgac cagggagggc
      241 agcggccagc gttcccccgg cttttcgcgg gcgaagcggg cgccgttcgg agcgacgacg
      301 tcgtccggcc ggcccgagcg ctcgtaggcg agcgggtcga cgcagaaggt gcgcatcacc
      361 gacgggtaga ggctcttgaa gtcgagcacg acgacgccat ctcggtagcc ggcctcagcc
      421 ggcagcaccg tgccgccggc cgctccggag acccgccgcc cctgctcgaa cggcggcggc
      481 gctaccgacc gatcgcggag ggcttggccg taggcccgct cgaaggcggc gacgctcgtc
      541 caggcccgct cgaggccgac gccgaccagg gccgcccgag cgaccgtcag cgcgaagagg
      601 cccgtgcggt ccaggatctg cggcacgagc tcggcgtcga gcgcggcgta gtcggcgaag
      661 gcggacgggt cgtcgcggta cgcgcggtcg agccactcga gcttggcccg gccgcgtagg
      721 gccgagccct tgccctgccc gagcgcgcgc cgcgccaccg tctctaagct gtagtcgtcg
      781 tagcgctccg gaccggaacg ggcgacgcgc atcgcgtcga tgacctggcg gcccggcaca
      841 acggcggtgg cggtccggcc gcggcccttg ggcaacacgc gggcgccgtc gctcgagcgg
      901 ccaatgtcga gggccaggcc gttggcctcg gcccgctcga cgagccgggc caggtcgaag
      961 tcgacgacgt tccagccggt gatcacgtcg gggtcgaggg ccaggacgcg ctccgacagg
     1021 gcgcgcacca gggaagcctc gtcggggcag ccgatagcct catagccttg agcctggccg
     1081 gccgcgagcg cctcgcggcc gacggcgccc ggcgccgcga cgaccaacac tatcgtcgac
     1141 ccttgcctcg tcacgagccc gccaccggac ggcgaccagt cagccatcga aaggctggcc
     1201 gcgcggacgg agcggtccga ctcgtccgtc tcgatgtcca gggacaggac cgacagggga
     1261 gcggcggcgt ccgggtcggc ctcgacgagg gcgtccgcga gcacgagccc gacacggcgg
     1321 cccgctcgct cgtcgccgga gacgcgcacg ccgagggcca ggccccgatc ggcccggtag
     1381 agctcggcta tcgggccgga gccatgcacg gccaggctcg cccgctccag ggccgcgacg
     1441 gcgtcccggt aggctccgaa gctcggcgcg gtccacgaaa ggagcccgcg cccgtcgata
     1501 gcctcgagag cgcacggtgc gaagcggcac gctaccgccc gcgcccgggg agacgcggcc
     1561 tcgtcggcgg ctacgtagac gaccggcctc caggacgcgt cctccgccgc gaaggaacgg
     1621 ccgtcttcga gccggcccac gacgaagacg cggtcgcggc ggtagtcggc gtagacctgc
     1681 gccgccacgc ccc
//
LOCUS       DAOPHV010000606         1687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_103041_length_1687_cov_3.658701, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000606 DAOPHV010000000
VERSION     DAOPHV010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103041_length_1687_cov_3.658701"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="PLQ29_12565"
     CDS             <1..1084
                     /locus_tag="PLQ29_12565"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPG87522.1"
                     /translation="DYLVKPFDPDELVIRLRKAVRDRRLSADREASRRPAVDRTRMVG
                     DSPAIKEALRLIERAAPTPATVLITGESGTGKEVVARLIHERSGRQGAFVAVNLGAIP
                     ESLLESELFGYEKGAFTGAAERKTGLFELADGGTLFLDEIGEMPAHMQAKLLRALQER
                     KVTRLGGTKGVPVDARVLAATNRDLEAMVASGSFREDLYYRVNVVRIHLPPLRERPGD
                     AVALAAYFVETLAPSLGRRNARLAQSALRLISGYPFPGNVRELSNAVERALILAEGDE
                     LQADDFELGPRSHGRAVEGRSPGEQAERSFVSEVGEALSLAELERRAILEALRRNGGR
                     REASALELGITRRTLFNKLNEYGYTD"
     gene            complement(1091..>1687)
                     /locus_tag="PLQ29_12570"
     CDS             complement(1091..>1687)
                     /locus_tag="PLQ29_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87523.1"
                     /translation="LFDEPLSSLDAALRKRLRFDIVEEQRRLGFTAVYVTHDLEEAMA
                     VGDRLAVMADGRVLQCGPPVELWERPVSAEVAAFMGSGPCLPVIRVERDGCLLVAVTA
                     AGRFVVPPSSVSVPGAWLFFDRSSITPCAAAPGDLGVFSVRCVRLDYAGDSLECVVEA
                     EGAGLSSLRLPKSTRARVGDLISLRVPASAVRLLPPSA"
BASE COUNT          305 a          557 c          588 g          237 t
ORIGIN      
        1 cgactacctg gtcaagccct tcgatccgga cgaactggtc atacgcctcc gcaaggccgt
       61 acgcgatcgt cgcctgagcg ccgaccgcga ggcttcgagg cgcccggcag tcgaccggac
      121 caggatggtc ggcgactcgc ccgccatcaa ggaagcgctc cggctgatag agcgagcggc
      181 gcccacgccg gccaccgtgc tgatcaccgg ggagagcgga acgggtaagg aggtggtagc
      241 gaggctgatc cacgagcgtt ccggtcgcca gggagccttc gtagctgtga acctcggcgc
      301 catcccggaa agcctgctcg aaagcgagct gttcggctac gagaagggtg ccttcaccgg
      361 cgcagcggag cgcaagaccg gcctcttcga gctggccgac ggcggcacgc tgttcctcga
      421 cgagataggc gagatgccgg cccacatgca ggccaagctc ctcagggccc tccaagagcg
      481 caaggtgacg aggctcggcg gcacgaaggg cgtaccggtc gacgccaggg tcttggcggc
      541 gaccaatcgc gacctcgagg cgatggtcgc ctcaggctcc ttcagggagg atctatatta
      601 tcgcgtcaac gtggtccgga tacatctccc accgctaagg gaacgccctg gcgacgccgt
      661 agcgttggcc gcttacttcg tcgagacgct cgcgccgtcg ctcggtcgac ggaacgcccg
      721 gctcgcgcag agcgctctcc ggctgatctc cggctatccg ttcccgggga atgtccgcga
      781 actgtccaac gccgtcgagc gcgcgctgat cctggccgag ggcgacgagc tccaggccga
      841 cgattttgag ctcgggccga ggtcgcacgg ccgggcggtc gaaggccggt cgccaggcga
      901 acaggccgag cggtccttcg tctccgaagt cggtgaggcc ctgtcgctcg ccgagctcga
      961 gagacgggcc atcctcgagg cgttgcgtcg caacggcggc aggcgcgagg ccagcgcgct
     1021 ggagctgggc atcaccaggc ggacattgtt caataagctg aacgagtacg gctataccga
     1081 ttgaggcgga ctaggccgac gggggaagga gcctgacggc cgacgcgggc acgcgcaggc
     1141 tgattaggtc accgacgcgc gctcgggtcg acttgggtag gcgtagcgac gacagacccg
     1201 ctccttcggc ctccacgacg cactcgagcg agtcgcccgc gtagtcgagg cggacgcatc
     1261 ggacgctgaa gacgcccaga tcacccggcg ccgcggcgca cggcgtgatc gacgatcggt
     1321 cgaagaacag ccatgcgcct gggaccgaca ccgaggacgg tggtacgacg aatcgaccgg
     1381 ctgccgtgac ggccacgaga aggcagccgt cgcgctcgac gcggatgacg ggcaggcagg
     1441 ggcccgagcc catgaaagcg gccacctccg cgctaaccgg ccgttcccac agttcgacgg
     1501 gcgggccgca ttgcagaacc ctgccgtcag ccatcaccgc gaggcgatcg ccgacggcca
     1561 tcgcttcctc gaggtcgtgg gtcacgtaga ccgctgtgaa gccgagtctg cgttgctcct
     1621 ccacgatgtc gaagcgcagg cgcttacgta gcgcggcgtc gagagacgaa agaggctcgt
     1681 cgaacag
//
LOCUS       DAOPHV010000607         1685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_103229_length_1685_cov_3.635583, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000607 DAOPHV010000000
VERSION     DAOPHV010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103229_length_1685_cov_3.635583"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLQ29_12575"
     CDS             <1..240
                     /locus_tag="PLQ29_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HPG87524.1"
                     /translation="MGTLPDTGLDPAMLVRLANARMPFGKYAGRLLVDLPEAYVVWFS
                     HKGYPKGELGELLASLYAIKENGLEPLLRPLVRGS"
     gene            complement(227..937)
                     /locus_tag="PLQ29_12580"
     CDS             complement(227..937)
                     /locus_tag="PLQ29_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HPG87525.1"
                     /translation="MAAHGFGSGSRTTMGRAGRAMLGAAVAAILSASATGCWTPTRRP
                     AESVFPLPAFECYEPAAVSETQRLIADGGRTLAGRKALIVHGVSYPLDCTGLVRAAYG
                     FASIDLAYRFDDYAGNGVRRLYQTLRDEGLLYAVRYPAPADLVFWDDTYDADGDGRPG
                     DELTHVGVVLSVGTDGSVEYLHYHYRLGPVIERMNLTRPDDPANATLRMRGSPDGPGT
                     NAAQLFRAFGKGYLLKSP"
     gene            complement(940..1524)
                     /locus_tag="PLQ29_12585"
     CDS             complement(940..1524)
                     /locus_tag="PLQ29_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPG87526.1"
                     /translation="MTTQSAASDPRAILLEALDDYRSARPEERDTCDSFIAFVASRAD
                     CLRRSCLEGHVTGSAFVVDPELRMTLLVHHAKLDKWLQPGGHCEPGEIVAETAERETL
                     EETGVAASPRSRAIFDIDVHPIPARGSEPAHSHYDVRYLLVAEPGATTVSDESRAVEW
                     VGLDEAERRNPEPSITRMAAKARALRGVAPRSVG"
BASE COUNT          252 a          568 c          597 g          268 t
ORIGIN      
        1 atgggaacgc tgccggatac gggcctcgac ccggcgatgc tcgtccgcct ggccaacgcg
       61 cgcatgccct tcggcaagta cgctgggcgc ctgctcgtcg atctgccgga agcctacgtc
      121 gtctggttct cgcataaagg ctaccccaag ggcgaactcg gcgagctgct cgcttcgctg
      181 tacgcgatca aggagaacgg cctggaaccg ctcctgcggc cgctggtcag gggctcttga
      241 gcaggtagcc cttgccgaag gcgcggaaca gctgggcggc gttggtcccg gggccgtccg
      301 gcgagccgcg catcctgagc gtcgcgttgg ccgggtcgtc cggtctggtc aggttcatcc
      361 gctcgatgac cggcccgagt cggtagtggt agtgcaggta ctcgacgctc ccgtcggtcc
      421 cgacggacag tacgacgccg acatgggtca gttcgtcgcc ggggcggccg tcgccgtcgg
      481 cgtcgtaggt gtcgtcccag aacacgaggt cggccggcgc gggatagcgt accgcgtaga
      541 gtagcccctc gtcgcgtagc gtctggtaca gcctgcggac gccgttgccc gcgtagtcgt
      601 cgaagcggta ggccaggtca atcgacgcga agccatacgc ggcgcggacg agtccggtac
      661 agtcgagggg gtaactgact ccatggacga tgagggcctt cctgccggcg agcgtacgcc
      721 cgccgtcggc tatcagccgc tgggtctccg agacggcggc cggctcgtag cactcgaacg
      781 cgggcaacgg gaagacactc tcggcgggcc gcctcgtcgg cgtccagcag ccggtcgccg
      841 acgccgagag gatagccgcg accgcggcgc cgagcatcgc tcggcccgct cggcccatcg
      901 tcgttctgga accggaaccg aaaccgtgcg cggccacggc tagcccacgc tcctcggagc
      961 gacgcctcgg agcgcccgcg ccttggccgc cattcgcgtt atcgacggct cggggttgcg
     1021 gcgctccgcc tcgtcgaggc cgacccactc gacggcccgc gactcgtcgc tgaccgtcgt
     1081 cgcgcccggc tcggccacga gcaggtacct gacgtcgtaa tggctatggg ccggctcgct
     1141 tccccgcgcg ggaatgggat gcacgtcgat atcgaagatg gcccggctcc gcggcgacgc
     1201 cgctaccccg gtctcctcga gcgtttcgcg ctcggctgtc tcggcgacga tctctcccgg
     1261 ctcgcagtgg ccgccgggct ggagccactt gtcgagcttg gcgtgatgga cgaggagggt
     1321 catgcgtagc tcggggtcga cgacgaaggc ggacccggtg acgtggccct cgaggcagga
     1381 ccgtcgcagg cagtccgcgc ggctcgcgac gaaggcgatg aacgagtcgc aggtatcgcg
     1441 ttcctcgggg cgcgccgaac ggtagtcgtc gagggcctcc agcagtatcg ccctcgggtc
     1501 ggatgcggca gattgcgtcg tcataatccc aggacctccg gtagggcggt tatggtcggc
     1561 caggccatgt acagcgagaa gccgagcacc agcaggtacg ataccagttc gagcaggtcc
     1621 gagaccagcc cgatcgtctt ctcgcgggcc ttgaggctgc cgaatagccc gacgcgaccg
     1681 gcgta
//
LOCUS       DAOPHV010000608         1673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_104358_length_1673_cov_3.010507, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000608 DAOPHV010000000
VERSION     DAOPHV010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104358_length_1673_cov_3.010507"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..>1673)
                     /locus_tag="PLQ29_12590"
     CDS             complement(124..>1673)
                     /locus_tag="PLQ29_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HPG87527.1"
                     /translation="TAMLGDAAFKELSEAIIAANTTKVDVVSGASESSAGFVAAVEDA
                     LAKAGVVLAAKKGPAKAAPVVFDKDYDVVVIGAGGAGLSAAISAAQSGAKVIIVEKMA
                     MVGGNTVRATGGMNAAGTEAQAKAGIADSVESFYKDAMKGGYEKNDPVLVRKLAEESA
                     GSVAWLTSLGADLSDVGRLAGASVNRAHRPTGGAKVGPEIVETLNATVVKTAGVTVYT
                     KTRATAILKDKSGAVTGIKVIAADGREYDIKAKAVIIATGGFGANNDMAASYVPSLKG
                     FATTNHPGATGDGILLAEAAGAALVDMKEIQTHPTHAPDKEMITEAVRGNGAILVSKE
                     GARFVDELATRDVVSAAVLAQTGKTAFLVFDQSIRASLKAIEGYAKLGIVVEGDTPEA
                     LAAKIGSDPARLAATLAAYNGFVAAKRDAEFGRADMPRAVSVGPFYAIEITPAIHHTM
                     GGVKIDAEARVIAASGSPIVGLWAAGEVTGGVHGGNRLGGNALADIVTFGRIAGNGAA
                     AYATSSR"
BASE COUNT          215 a          538 c          622 g          298 t
ORIGIN      
        1 gctcgaggtc ccgcgcggag gttgacaaga gttccatgcg cggattatac gccccgcgcc
       61 ttctcggggc aatcggtgag gccgccccga cggggcggcc tcggtatcag gcgggtctag
      121 cgcttagcgt gaggacgtgg cgtaggccgc ggcgccgtta ccggctatgc ggccgaaggt
      181 gacgatgtcg gcgagcgcgt tgccgccgag gcggttgcct ccgtggacgc cgccggtgac
      241 ctcgcccgcg gcccagaggc cgacgatcgg ggagccggag gcggcgatga cgcgggcctc
      301 ggcgtcgatc ttgacgccgc ccatcgtatg gtggatggcc ggggttatct cgatggcgta
      361 gaacggcccg acggacacgg ccctgggcat gtcggcgcgg ccgaactcgg cgtcgcgctt
      421 ggcggcgacg aagccgttat aggcggcgag cgtggcggcc agccgggcgg ggtcggagcc
      481 tatcttggcg gccagagcct cgggggtgtc gccctctacg acgatgccaa gcttcgcgta
      541 gccctctatg gccttgaggc tggcgcgtat cgactggtcg aacacgagga aggcggtctt
      601 gccggtctgg gcgagcacgg ccgcggacac gacgtcgcgg gtagccagct cgtccacgaa
      661 gcgggcgcct tccttgctga cgaggatggc gccgttgccg cgtacggcct cggttatcat
      721 ctccttgtcg ggggcgtgcg tcgggtgggt ctggatctcc ttcatgtcga cgagggccgc
      781 gccggcggcc tccgcgagga gaatgccgtc gccggtggcg ccggggtggt tcgtcgtcgc
      841 gaagcccttg agggacggca cgtaggacgc ggccatgtcg ttgttggcgc cgaagccgcc
      901 cgtggcgatg atgacggcct tggccttgat gtcgtactcg cgtccgtcgg cggctatcac
      961 cttaataccg gtgacggcgc ccgacttgtc cttgaggata gcggtcgccc tggtcttggt
     1021 gtacaccgtg acgccggccg tcttgacgac ggtcgcgtta agggtctcga cgatctccgg
     1081 tccgaccttg gcgccgccgg tgggcctgtg cgcgcggttg acgctggcgc cggcgaggcg
     1141 tccgacgtcg gagaggtcgg cgccgagcga ggtcagccac gcgacggagc cggcggattc
     1201 ctcggccagc ttgcgcacga ggacggggtc gttcttctcg taaccgccct tcatcgcgtc
     1261 cttatagaag gattcgacgc tatcggctat accggccttg gcctgggcct cggtgcccgc
     1321 ggcgttcatg ccgccggtcg ccctgaccgt attgccgccg accatggcca tcttctcgac
     1381 gattatgacc ttggcccctg actgcgccgc ggatatggcc gcggacaggc cggcgccgcc
     1441 ggcgccgatg accacgacgt cgtagtcctt gtcgaagacg acgggcgcgg ccttggcggg
     1501 gcctttcttg gcggccagca cgacgccggc cttggccagg gcgtcctcca cggcggcgac
     1561 gaagcctgcg ctcgactctg acgcgccgga gactacgtcg accttggtgg tgttggcggc
     1621 tatgatggcc tcggacagct ccttgaacgc ggcgtcgccg agcatggcgg tct
//
LOCUS       DAOPHV010000609         1670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_104634_length_1670_cov_3.702167, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000609 DAOPHV010000000
VERSION     DAOPHV010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104634_length_1670_cov_3.702167"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="PLQ29_12595"
     CDS             complement(<1..1062)
                     /locus_tag="PLQ29_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPG87528.1"
                     /translation="MAKTKYVYYFGDGKAEGDAKMKEALGGKGANLAEMTNLGIPVPP
                     GFTVSTDVCAAFYENKRKYPDGLEAEVAEHLARLEKSMGKKLGDAVDPLLVSVRSGAA
                     QSMPGMMDTILNLGLNDKAVEGLAKKTGNPRFAYDSYRRFIQMYGDVVLEVPHDEFEH
                     ALAAAKKAKKVELDTGLSADDLKKLVETYKAIVKKHTKKDFPQNPQDQMWGAMGAVFG
                     SWMNERAIKYRDLNGIKNLKGTAVNIQSMVFGNWGDDSGTGVCFSRDPSTGKNEFYGE
                     FLMNAQGEDVVAGIRTPEKIASLAKKNAAVYKQLVGIKDNLEKHFRDMQDMEFTVQQG
                     KLFILQTRNGKRTGAAAVKM"
     gene            complement(1176..>1670)
                     /locus_tag="PLQ29_12600"
     CDS             complement(1176..>1670)
                     /locus_tag="PLQ29_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87529.1"
                     /translation="AEYVALNVMELAANAENNNLKREAKAIFKGAVDMNAVLFDPNVR
                     HQVLESLQHRGELVSISWKLGSKGTSIGTQGRLHITIYNKESEYEKMKEAFDEKKNAD
                     LKKRTLQDFYKALPEGESNTDLGLYYLSYLSEACEKVNVKFESFVNQISGSDLTVITM
                     AINL"
BASE COUNT          268 a          481 c          539 g          382 t
ORIGIN      
        1 catcttgacc gccgcggcgc cggtgcgctt gccattacga gtctgtagga tgaacagctt
       61 gccctgctgg acggtgaact ccatgtcctg catgtcgcgg aagtgcttct cgaggttgtc
      121 cttgatgccg acgagctgct tgtagacggc ggcgttcttc ttggcgaggg aggcgatctt
      181 ctcgggggtg cggatgccgg cgacgacgtc ctctccctgg gcgttcatca ggaactcgcc
      241 gtagaactcg ttcttgccgg tcgacgggtc gcggctgaag cagacgccgg tgccggagtc
      301 gtcgccccag ttaccgaaca ccatcgactg gatattgacg gcggtgccct tgaggttctt
      361 tatgccattg aggtcgcggt acttgatggc ccgctcattc atccaggagc cgaagacggc
      421 tcccatggcg ccccacatct gatcctgcgg attctggggg aaatccttct tggtgtgctt
      481 cttgacgatg gccttatagg tctcgacgag cttcttgagg tcatcggcgg agaggccggt
      541 gtcgagctcg accttcttgg ccttcttggc ggccgcgagc gcgtgctcga attcgtcgtg
      601 cggcacctcg aggacgacgt cgccgtacat ctggatgaaa cggcggtacg aatcgtaggc
      661 gaagcgggga ttgccggtct tcttggccag gccctcgacg gccttgtcgt tgaggcccag
      721 gttgaggatg gtgtccatca tgccgggcat ggactgggcc gcgccggagc gtacggatac
      781 gagcaggggg tcgacggcgt cgccgagctt cttgcccatg gacttctcga gcctggcaag
      841 gtgctccgct acctcggcct cgaggccatc ggggtacttg cgcttgttct cgtagaatgc
      901 cgcgcagaca tcggtggaca ccgtgaatcc cggggggacc gggatgccca ggttggtcat
      961 ctcggcgagg ttggcgccct tgccgccgag cgcttccttc atcttggcgt cgccttcggc
     1021 cttgccgtcg ccgaagtagt atacgtactt ggtctttgcc atcgagtaaa atcctccgtt
     1081 caacccatga ggctatagga aaccacagcc tctgtcaaga aaccgtaagg gagggtatcg
     1141 agggacggag ggtttcgggc gaagtgcccc tcgggctaca ggttgatggc catcgtgatg
     1201 acggtcaggt cggagccgga gatctggttg acgaaggact cgaacttgac gttgaccttc
     1261 tcgcaggcct cggacaggta ggacaggtag tagagcccga ggtcggtatt cgactcgccc
     1321 tccggcaggg ccttgtagaa gtcctgcagg gtgcgcttct tgaggtcggc gtttttcttc
     1381 tcgtcgaacg cctccttcat cttctcgtac tcggattcct tgttatagat cgttatatgc
     1441 agccggccct gggtcccgat cgaggtgccc ttggagccga gcttccatga tatggacacc
     1501 agctcgcccc ggtgctggag cgactcgagc acctgatggc gcacgttcgg gtcgaacagg
     1561 acggcgttca tgtcgactgc gcccttgaag atggccttgg cctcgcgctt caggttattg
     1621 ttctcggcgt tggccgcaag ctccatcacg ttgagcgcca catactcggc
//
LOCUS       DAOPHV010000610         1665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_105095_length_1665_cov_3.113665, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000610 DAOPHV010000000
VERSION     DAOPHV010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105095_length_1665_cov_3.113665"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1528
                     /gene="yqeB"
                     /locus_tag="PLQ29_12605"
     CDS             <1..1528
                     /gene="yqeB"
                     /locus_tag="PLQ29_12605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HPG87530.1"
                     /translation="TPRGKAKMLVTASGVVAGTVGGGAVEARVIEEARRAIALDRAIM
                     LEYSLDHGPGAASLPMECGGSMRVFVEVFGSRPRLVIVGAGHVGQAIARAAKAIGYRV
                     AMVDDRPDYATAERLSCADELYVDAELAAAMAAAPIGALDCVVVSTPSHRSDEQAIRH
                     YAGSDCRYLGVLGSRRKVTAMFSRMRADGVPEERIAEVRAPIGLDLGAETPEEIAVSV
                     LAEVMAVVSGRDGAPLAGRQSDLIVVRGAGDLATGVIVRLKAAGFRVAALEIERPTAI
                     RRSVALCEAVYSGEARVEGVLARRVAGLEEARSAIADGVVPILVDPDCRYVRALAPVA
                     LVDATLSKRNTGVRRGMAPAVIGLGPGFEAGGDVDAVIETQRGHDLGRVILRGKAADD
                     TGVPGELGGKSAERVIKSTAAGVVEAIASIGQLVEIGDPVIGVRGPDGLTVVKTTIAG
                     VVRGAIRPGLEVPVGMKIADVDPRGRVDSCHSVSDKARSIGGAVLEAILALKGRQKRD
                     "
BASE COUNT          237 a          548 c          636 g          244 t
ORIGIN      
        1 gacgccgcgc ggcaaggcca agatgttggt cacggcctcc ggcgttgtgg cgggaacggt
       61 cggcggcggc gcggtcgagg cccgggtcat cgaggaagcc aggcgggcca tcgccctgga
      121 cagggccatc atgctcgagt actcgctcga ccacggcccg ggcgcggcca gcctgccgat
      181 ggaatgcggt ggctctatgc gcgtattcgt cgaggttttc ggctcgaggc cgcggctcgt
      241 catcgtcggg gcgggacacg tcggccaggc aatcgccagg gccgccaagg ccatcggtta
      301 ccgcgtcgct atggtcgacg accggcccga ctacgcgacg gccgaacgcc tatcgtgcgc
      361 cgacgaactg tacgtcgacg ccgagctcgc ggcggcgatg gcggccgcgc cgatcggcgc
      421 cctggattgc gtcgtcgtct cgacgccgtc gcaccgcagc gacgagcagg cgattcgaca
      481 ctatgccggg tccgactgcc gttatctcgg cgtgctcggc tcgaggcgca aggtgaccgc
      541 gatgttctcg cgcatgcgcg cggacggcgt gcccgaggag cgaatcgcgg aggtcagggc
      601 tcctatcggc ctcgacttgg gcgccgagac gccggaggag atagccgtat cggtgctggc
      661 cgaggtcatg gccgtcgtat cgggccgcga cggggctccg ttggcaggcc gtcagtccga
      721 tctgatcgtg gtgcgaggcg ccggcgactt ggcgacgggc gtcatcgtca ggctcaaggc
      781 ggccggtttc agggtagccg ccctcgagat agagcggccg acggctatca ggcgctcggt
      841 cgccctgtgc gaggccgtgt actcgggcga ggcgcgagtc gagggcgtgc tcgcgaggcg
      901 ggtcgccggc ctcgaggagg cccgttccgc gatcgccgac ggcgtcgtgc ctatcctcgt
      961 cgacccggac tgccgctacg tccgcgccct tgcgcccgtc gccctcgtcg acgcgacgct
     1021 cagtaagcgc aataccggcg tccgccgcgg catggcgcca gcggtgatag gactcgggcc
     1081 gggcttcgag gcgggcggcg acgttgatgc ggtgatcgag acgcagcgag gccacgacct
     1141 cggcagggtc atcctccgcg gcaaggcggc cgacgacacc ggtgtgcccg gcgagctggg
     1201 cggcaagagc gccgagcgcg tgatcaagtc tacggcggcc ggcgtcgtcg aggcgatagc
     1261 gtcgataggc cagctggtcg agatcggcga cccggtgata ggcgtgcgtg ggcccgacgg
     1321 gctgaccgtg gttaaaacca cgatagccgg cgtcgtgcgc ggcgcgatcc ggcccggcct
     1381 cgaggtgccc gtcggtatga agatagcgga cgtcgacccg cgcggccgcg tggatagctg
     1441 ccacagcgtc tcggataagg cccgctcgat aggcggcgcc gtcctcgagg cgatactcgc
     1501 gctcaagggg cgccagaagc gcgactaggc ccggttagag gctcggctag gcccggttag
     1561 aggcccaggc cctcgatata cgctacggcg cgccgcttga cgagcgcctc ggctggctcg
     1621 ccgggcggca gggccgtggc cgggatgaag ccgccgccca tgtgg
//
LOCUS       DAOPHV010000611         1664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_105199_length_1664_cov_2.830329, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000611 DAOPHV010000000
VERSION     DAOPHV010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105199_length_1664_cov_2.830329"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PLQ29_12610"
     CDS             complement(<1..658)
                     /locus_tag="PLQ29_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87531.1"
                     /translation="MPMTVFVDSILESNLGYAALFICVGVGFLFLMQVDSGEPGPGLW
                     SLGFFLNALGFLLWSGTVAIPTVLYLLAGEVFHVAGFMMMITGLYRFTGRAYTPAFFA
                     LVAAWLLIWAVSIVMLRYFVYPAGIALKLLRAVLFVVAGFMALDRRGDGSRFGSRVAG
                     YSLIAWGAYTVVFALVRFDSLLNLFYGFLTGLQVLSAFGMVAMVVDRVQAKAEASERR
                     A"
     gene            complement(807..1253)
                     /locus_tag="PLQ29_12615"
     CDS             complement(807..1253)
                     /locus_tag="PLQ29_12615"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HPG87532.1"
                     /translation="MRIIECAERDALSVRYRTPVAKLSESMGPAYGEIAGYMAKNGIP
                     FAGPPYAMYYNMDMDDLDVEIGFPVGAPAKGEGRVRPGKLPGGRHATATHGGSYETMA
                     KTYDALTAFCKERGVELEERMYEEYLNSPEEVPPEKLETVIHFLLR"
     gene            complement(1336..1548)
                     /locus_tag="PLQ29_12620"
     CDS             complement(1336..1548)
                     /locus_tag="PLQ29_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87533.1"
                     /translation="MKTLRRRHSLLPVLALLAAALRAEPCVVISEIMYHPADPQEALE
                     FVELLEPERLRAVIRDAAGKIRSLYE"
BASE COUNT          311 a          538 c          559 g          256 t
ORIGIN      
        1 cggcgcgtcg ctcgctggcc tcggccttgg cctgtacgcg gtcgacgacc atggccacca
       61 tgccgaaggc cgacaggacc tggagcccgg tcaggaagcc gtagaatagg ttgagcaagg
      121 aatcgaagcg gaccagcgcg aagaccaccg tgtacgcgcc ccaggctatc aggctgtagc
      181 cggcgacgcg gctgccgaag cgcgagccgt ccccgcgccg gtcgagcgcc atgaagccgg
      241 cgacgacgaa cagtacggcc cgtaatagct tgagcgcgat gcccgccggg tagacgaagt
      301 accggagcat gacgatggat acggcccaga tcaggagcca ggcggcgacg agcgcgaaga
      361 aagccggcgt gtaggcgcgg ccggtgaacc gatagagccc ggttatcatc atcatgaagc
      421 cggcgacatg gaagacctcg cccgccagga ggtacaggac ggtcggtatc gccaccgtac
      481 ccgaccagag caggaagccg agcgcgttca ggaagaagcc gagcgaccag agcccagggc
      541 ccggttcgcc cgagtccacc tgcatgagga acaggaagcc cacgcccacg cagatgaaca
      601 gcgcggcgta cccgaggttg ctctccagga tagaatcgac gaaaacggtc atcggcatac
      661 gccgctcctt gccctaggtg tctggtaatc acttttagct aggctggagg cgcggaacga
      721 tccgcggtcg acagaatacg tcgatactag catgctcggc gtttcagcac tagcccggcg
      781 gcgacggtac gccgccgggc tagcgctcac ctgagcagga agtggatgac cgtctcgagc
      841 ttctccggag gcacctcctc ggggctgttc aagtactcct cgtacatgcg ctcttcgagc
      901 tcgacgcccc gctccttgca gaaggccgtc agcgcatcgt aggtcttggc catggtctcg
      961 tacgagcccc cgtgcgtggc ggtcgcgtgc cgcccgccgg gtagcttgcc cggcctcacg
     1021 cggccctcgc ccttggccgg ggcgcctacc gggaagccta tctccacgtc caggtcgtcc
     1081 atgtccatgt tgtagtacat ggcgtagggc ggccccgcga acgggatgcc gttcttggcc
     1141 atgtagccgg cgatctcgcc gtaggcggga cccatgctct ccgacagctt ggcgaccggc
     1201 gtccggtagc gtaccgacag cgcgtcgcgt tcggcgcatt cgatgatcct catgctcgct
     1261 ctccttctgg aaccaggatg gacgcatggt aacgcgggat atacgacacc tctatgtcgg
     1321 ggttaatcgt accgcctatt cgtataacga gcgaattttc ccggccgcgt cgcggataac
     1381 ggcgcgtagg cgctcgggct cgagaagctc gacgaactcg agggcctcct ggggatcggc
     1441 ggggtgatac atgatctcgg agatgacgac gcacggctcc gcgcggagcg ccgccgcgag
     1501 gagcgcgagg acagggagca gggagtgtct ccggcgcagt gtcttcattc ggccatccgc
     1561 acaggggatc accgctagtc tacaccggag agcggcgcgg tcaacggaac ggccgcgcgg
     1621 cccgcgccga agggctgccc gccggggcgc cgcgctaccc gagg
//
LOCUS       DAOPHV010000612         1663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_105268_length_1663_cov_3.177239, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000612 DAOPHV010000000
VERSION     DAOPHV010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105268_length_1663_cov_3.177239"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLQ29_12625"
     CDS             complement(<1..253)
                     /locus_tag="PLQ29_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87534.1"
                     /translation="MSVDPLSVSIASVFASVGLDAFAVAGVDTLVEFARRADSAAVDR
                     YGLLESSGAVVAALPYDPRPSPLPGDDVSGDDVSGDDVSG"
     gene            complement(250..1482)
                     /locus_tag="PLQ29_12630"
     CDS             complement(250..1482)
                     /locus_tag="PLQ29_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPG87535.1"
                     /translation="MDNPLQLSFVNFKRDSYIIVEGKQNADRFFIIKSGKVRISKEVE
                     VVEEEGGNIYSPGDFFGVISTMSSHSHIETAQAITDVTLISVHRDQYGLLIEKNTPVA
                     MKIIMGFSKRMRYLDEALTRITLKKTAEIDPSHLYKVAECYARQSQFNQAYYAYYQYL
                     KYCPNGQHASAAKERMAKIKPYSKAVYLDGSTEEFNRYYPKDTMVFSEGMPGTELYII
                     QKGSVKITKIVDNTEVLLAVLKAGDIFGEMSLLENKPRSASAIAYEDAYLLAVNKQNF
                     ERMVQAQPQIVTRLTQLLAERIWFIYKQLANTLISDPLGRMYDALHIQLEKNRVPIKN
                     GATYAFEFGPKELVNMVGLPIAEGNAVVQQLLANKLFRLIDNKIVVQDMSEIVKQAEY
                     YRKMQKIEKARKEAQAAR"
BASE COUNT          283 a          490 c          535 g          355 t
ORIGIN      
        1 cgccggacac gtcgtcgccg gacacgtcgt cgccggacac gtcgtcgccg gggagcggcg
       61 acggccgagg gtcgtacggc agcgccgcta cgaccgcgcc cgacgactcg aggaggccgt
      121 agcgatccac ggccgctgag tcggcgcggc gggcgaactc gacgagggta tcgacaccgg
      181 cgaccgcgaa ggcgtccaag ccgaccgaag cgaacaccga ggctatggat acggagagtg
      241 gatcgacgct caacgagccg cctgggcctc tttgcgggct ttctctatct tctgcatctt
      301 gcggtagtac tcggcctgct tgacgatttc gctcatgtcc tgcacgacta tcttgttatc
      361 gatcagcctg aacagcttgt tcgccaggag ctgctggacg accgcgttgc cctcggcaat
      421 cggcaggccg accatgttga ccagctcctt cggcccgaac tcgaaggcgt aggtcgcgcc
      481 gttcttgata ggcacccggt tcttctcgag ctggatatgg agggcgtcgt acatgcgtcc
      541 gagcgggtcg gagatcagcg tgttggccag ctgcttgtag atgaaccaga tgcgctcggc
      601 gagcagctgc gtcagcctgg tgacgatctg cggctgagcc tggaccatgc gctcgaaatt
      661 ctgcttgttg acggcgagca ggtaggcgtc ttcataggct atcgcgctcg cggacctcgg
      721 cttgttctcg agcagggaca tctccccgaa gatgtcgccg gccttgagca cggcgagcag
      781 cacctcggta ttgtcgacga tcttggtgat cttgaccgag cccttttgga tgatatacag
      841 ctcggtgcct ggcatgccct ccgagaacac catcgtatcc ttcggatagt agcggttgaa
      901 ctcctcggtg gagccgtcca ggtagacggc cttggagtac ggcttgatct tggccatacg
      961 ctccttggcc gccgaggcgt gctggccgtt cgggcagtac ttgaggtact ggtagtaggc
     1021 gtagtaggcc tggttaaact ggctttgcct ggcgtagcac tccgccacct tgtacaggtg
     1081 ggacgggtcg atctccgcgg tcttcttgag ggtgatgcgg gtcagcgcct cgtcgaggta
     1141 gcgcatgcgc ttcgagaagc ccatgatgat cttcatcgcg accggggtat tcttctcgat
     1201 caggaggccg tactggtcgc gatgcacgct gatgagcgtc acgtcggtga tggcctgggc
     1261 cgtctcgata tggctatggc tggacatggt ggagatgacg ccgaagaagt cgccgggcga
     1321 gtagatgttg ccgccctctt cctcgacgac ctccacttcc ttggagatcc gcaccttgcc
     1381 ggacttgatt atgaagaagc ggtcggcgtt ctgcttgccc tcgacgatga tatacgagtc
     1441 tcttttgaaa ttgacgaagg acagctggag cggattatcc atgccgttac cgtttctccc
     1501 gtcgcgaagc tctctcgagt ataggtacgc cccggggggt tgtcaattgc cgatagtccg
     1561 gcgccccggg gcgcccgggg aacatccccg ggatcgctac ggcccagtgt atcacagatc
     1621 ggccggaagg ccgaagcgtc cggtcaattt ctgcacctcc gcc
//
LOCUS       DAOPHV010000613         1662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_105337_length_1662_cov_5.023647, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000613 DAOPHV010000000
VERSION     DAOPHV010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105337_length_1662_cov_5.023647"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PLQ29_12635"
     CDS             <1..864
                     /locus_tag="PLQ29_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPG87536.1"
                     /translation="ATDFAVVFAAMGVKHDVARFFMRSFEETGALDNVALFLSLADAP
                     SIERIITPRTALTLAEHLAYDRGLHVLVVMTDMSNYCESLREISTLRGEIPSRKGYPG
                     YLYSDLAELYERSGMIRGVKGSITQLPILTMPNDDISHPIPDLSGYITEGQIVFEREM
                     NGRGIYPPVAGLPSLSRLMKDGIGGGMTREDHPHLASQLFAAYSYVKDVRNLASVIGE
                     EELTPLDHQYMEFGEFFERRFVNQRRDEDRTIEQTLDLGWEALKLLPAEELHRVTDEE
                     LDAYYHGNGQS"
     gene            861..1499
                     /locus_tag="PLQ29_12640"
     CDS             861..1499
                     /locus_tag="PLQ29_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPG87537.1"
                     /translation="MRNVAPTKTNLIRLKDELKFARMGHELLDQKRNILVLELLNLVD
                     QAVEYDEKVDTALAKAYAGLEDSVLAMGRLKVGALAGAIDVSSTIALKQRRVMGVALP
                     VVRTEFAAGGPYYSPLDTSASIDNAVAAFRDALAMLGRSAELRVSIVRLAREVRKTIR
                     KVNALEKIAIPELDETLHYIQNRLEESERDMFVLMKMVKDRLSRPDSEVSQG"
     gene            1520..>1662
                     /locus_tag="PLQ29_12645"
     CDS             1520..>1662
                     /locus_tag="PLQ29_12645"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPG87538.1"
                     /translation="MVYVDGSEQSITAAQYAIVLAKTLGADLYALYVVDTRALAELVN
                     SRI"
BASE COUNT          316 a          517 c          552 g          277 t
ORIGIN      
        1 gcgacggact tcgcggttgt gttcgccgcg atgggcgtca agcacgacgt ggcgcgcttc
       61 tttatgcgct cgttcgagga gaccggagcg ctcgacaacg tggcgctgtt cctgtcgctc
      121 gcggacgcgc cgtcgatcga gcgcatcatc acgccgcgca ccgcgctgac cctggccgag
      181 cacctggcct acgaccgcgg cctccatgtg ctcgtcgtga tgaccgacat gtcgaactac
      241 tgcgagtcgc tacgcgagat ctcgacgctg cgcggcgaga taccgtcccg caagggctac
      301 ccgggctacc tgtactcgga tctcgcggag ctgtacgagc gctccggtat gatacgcggc
      361 gtcaagggct cgatcaccca gctgcccatc ctgacgatgc cgaacgatga catctcccac
      421 cctataccgg atctgtccgg ctacatcacc gagggacaga tcgtcttcga gcgcgagatg
      481 aacggccgcg gcatctaccc gccggtcgcc ggcctgccgt ccctgtcgcg cctgatgaag
      541 gacggcatcg gtgggggcat gacccgtgag gaccacccgc accttgcgag ccagctgttc
      601 gcggcctata gttacgtcaa ggacgtgcgc aacctggcgt ccgtgatcgg cgaggaggag
      661 ctgacgccgc tcgaccacca gtacatggag ttcggcgagt tcttcgagag gcgcttcgtc
      721 aaccagcgca gggacgagga tcggaccatc gagcagacgc tcgacctcgg ctgggaggcg
      781 ctcaagctgc tgccggctga ggagctgcac cgcgtgaccg acgaggagct tgacgcgtac
      841 taccacggga atgggcagtc gtgaggaacg tcgcgccgac caagaccaac ctgatacgcc
      901 tgaaggacga gctcaagttc gccaggatgg gccatgagct cctggaccag aagcgcaata
      961 tcctggtgtt ggagctgctg aacctggtgg accaggcggt cgagtatgac gagaaggtcg
     1021 acacggcgct ggccaaggcc tacgccggcc tcgaggactc ggtgctcgcg atgggcaggc
     1081 tcaaggtcgg ggcgctggcc ggcgccatcg acgtcagctc gacgattgcg ctcaagcagc
     1141 gccgcgtgat gggcgtagcg ttgccggtcg tccggaccga gttcgcggcg ggggggccgt
     1201 actacagccc gctcgatacg agcgccagca tcgacaacgc ggtggccgcc ttccgcgacg
     1261 cgctcgcgat gctcggccgg tccgccgagc tgcgcgtctc tatcgtcagg ctggccaggg
     1321 aggtgcgcaa gacgatacgt aaggtgaacg cgctcgagaa aatagcgata ccggaattgg
     1381 acgaaacgct gcattatata cagaacaggt tggaagagag tgagcgcgac atgttcgtcc
     1441 tcatgaagat ggtcaaggac aggctatcgc gaccggattc ggaggtatcg caaggatgac
     1501 gaggccaata cgcaagatca tggtctatgt ggacggctcc gagcagtcga tcacggcggc
     1561 ccagtacgcc atcgtgctgg ccaagacgct cggcgccgac ctctacgcgc tgtacgtcgt
     1621 cgatacgagg gcgctcgccg agctcgtcaa ctcgcggatc tt
//
LOCUS       DAOPHV010000614         1662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_105359_length_1662_cov_3.439950, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000614 DAOPHV010000000
VERSION     DAOPHV010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105359_length_1662_cov_3.439950"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1662)
                     /gene="dnaE"
                     /locus_tag="PLQ29_12650"
     CDS             complement(<1..>1662)
                     /gene="dnaE"
                     /locus_tag="PLQ29_12650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPG87539.1"
                     /translation="ITDIDPLAYALLFERFLNPERVSMPDFDVDFCYEGRGSVIDYVT
                     EKYGEDRVGQIITFGTLKAKAVIKDVARALDLSIDESNMITKLVPEDPKMTLAKALEL
                     EPKLRELAENPRYQELFEMARKLENKNRHTSFHAAGIVIGKTVLTDYVPLFKDPKTGI
                     VATQYTMDQLEECGLVKMDFLGLKTLTLIKNTLALIRRRGVDVVEDGIPDTDEATFRM
                     LGEGKSTSVFQFESDGMQGILRQAKPSSVEDLIALNALYRPGPMDNIPQFIDSKWGRT
                     PITYPHPSLEKYLKETYGVIVYQEQVMQVAQEVAGYSLGRADLLRRAMSKKKVEVLEK
                     EKAPFIEGALKRGYTKEKAQEIFDILVPFAGYGFNKSHAAAYSVVAYRTAWLKANYPA
                     EYMAANLTNEISNTDKLTQYISEARSMGIGILAPDINRSEATFSVSEGKIVYGLIGVK
                     GVGEAVVEAVVAEREKAGPYADFMDFLERVGVRAMNKRAIETLIQAGCFDTMGRGRAE
                     LALNLERAFDYAQKKAEAGAYGQTSLFEASSEEEFPPFRFEPCQEL"
BASE COUNT          259 a          527 c          523 g          353 t
ORIGIN      
        1 agctcctggc agggttcgaa acggaacggc gggaattcct cctcgctcga tgcctcgaac
       61 aggctcgtct gaccgtaggc gccggcctcg gccttcttct gcgcgtagtc gaaggcgcgc
      121 tccaggttga gcgccagctc ggcgcgcccg cgccccatcg tgtcgaagca gccggcctgg
      181 atcagcgtct cgatagcccg cttgttcatc gccctgacgc cgacccgctc gaggaagtcc
      241 atgaaatccg cgtagggacc ggccttctcc cgctccgcga cgacggcttc gacgaccgcc
      301 tcgccgacgc ccttgacgcc gatgagaccg tagacgatct tgccctcgga taccgagaag
      361 gtcgcctccg aacggttgat gtccggggcc agaatgccga tgcccatgct cctggcctcg
      421 gagatgtact gggtcagctt gtcggtgttc gatatctcgt tcgtcaggtt ggcggccatg
      481 tactcggccg gataattggc tttgagccag gccgtccggt aggcgaccac cgaatacgcg
      541 gccgcgtggc tcttgttgaa gccgtagccg gcgaacggca ccaggatgtc gaagatctcc
      601 tgcgccttct ccttcgtgta gccgcgcttc agggcgccct ctatgaatgg cgccttctcc
      661 ttctccagga cctcgacctt cttcttgctc atcgcgcgcc gtaggaggtc ggcgcggccg
      721 agcgagtagc cggcgacctc ctgggccacc tgcatcacct gttcctggta gacgatgacg
      781 ccgtaggtct ccttgagata cttttcgagt gacgggtgcg ggtacgtgat cggcgtacgg
      841 ccccatttcg agtcgatgaa ctgcggaata ttgtccatcg ggccggggcg gtacagggcg
      901 ttcagggcta tgagatcctc gacgctcgac ggcttggcct gcctgagtat gccctgcatg
      961 ccgtcggact cgaactggaa cacgctcgtg ctcttgccct cgccgagcat cctgaaggtc
     1021 gcctcgtcgg tgtcggggat gccgtcctcg accacgtcga cgccgcgtct gcgtatcagc
     1081 gccagggtgt tcttgatcag ggtcagggtc ttgagtccca ggaagtccat cttgaccagg
     1141 ccgcattcct cgagctggtc catcgtgtac tgcgtcgcga cgataccggt cttagggtcc
     1201 ttgaacagcg gcacgtagtc ggtcaggacg gtcttgccga tcacgatacc ggcggcgtgg
     1261 aagctcgtgt ggcggttctt gttctcgagc ttgcgcgcca tctcgaacag ctcctggtag
     1321 cgcgggttct cagcgagctc cctgagcttc ggctccagct cgagtgcctt ggcgagggtc
     1381 atcttcggat cctctggcac gagcttcgtg atcatgttcg actcgtcgat cgacaggtcg
     1441 agggcacgcg cgacgtcctt tatcacggcc ttggccttga gcgtgccgaa ggtgatgatc
     1501 tggccgaccc tatcctcgcc gtacttctcc gtgacgtagt cgatgaccga gccgcggccc
     1561 tcgtagcaga agtccacgtc gaagtccggc atcgacacgc gctcgggatt gaggaagcgc
     1621 tcgaacagca gggcgtaggc cagcggatcg atgtcggtga tg
//
LOCUS       DAOPHV010000615         1660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_105532_length_1660_cov_4.530218, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000615 DAOPHV010000000
VERSION     DAOPHV010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105532_length_1660_cov_4.530218"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="PLQ29_12655"
     CDS             <1..917
                     /locus_tag="PLQ29_12655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG87540.1"
                     /translation="SPADAAPPNDAPRSPADAAPPAAGTPAAGTPAIIELRDVRASSG
                     GFEILDGACVAFPEGLCSVVMGPAGSGKSTLLKVAAGLVVPDSGSVLYRGADLARFGR
                     KDEAAFRAATGFVFQDAALWADQNILMNVTMPLRVHKAWLGPSAMAELARETLGRLGY
                     DEGMAYRPAELSAGEQKLVSIARAVIHEPQIVLMDDPTASLDEDAAERVYALIAKLRE
                     RRRTVVIVANSSELAYRFADRLGIIKGGRLVAFGAYEETVGAAEAALSGSIARLKARG
                     ERVAEPGRERGPAPGLDAGSENSQAGDV"
     gene            921..>1660
                     /locus_tag="PLQ29_12660"
     CDS             921..>1660
                     /locus_tag="PLQ29_12660"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPG87541.1"
                     /translation="MKFKIKFADQIVGLFIIVALAFFAVIVVLIGANQRWFAKDYRFV
                     TSFPSAAGVAPGTSILMKGFTIGKIDKLRLNADNAVDADFHIYDTYYDKVREYSILEL
                     TVSPIGLGSQLLFHPGTGEARLEEGAFVPTADSIQGQSLIDQELVVIPPKDDTITRLL
                     ANVNPLLENTNKTIVTVNRTLTEVNRALAGQSSGPLGDIVGDVSVAAAALPGAMAGVG
                     GIVGDVESRVAILLDQAGSLLAELETIAG"
BASE COUNT          268 a          600 c          554 g          238 t
ORIGIN      
        1 ggtcaccggc cgacgccgcg cccccgaacg acgccccccg gtcaccggcc gacgccgcgc
       61 ccccggccgc cggtactccg gccgccggta ctccggcgat catcgagcta cgcgacgtgc
      121 gcgcctcgtc cggcggcttc gagatactcg acggcgcctg cgtcgccttc cccgagggcc
      181 tatgctccgt ggtgatgggg ccggccggct cgggcaagag caccctgctc aaggtggccg
      241 ccggcctcgt cgtgcccgac tcgggttcgg tgctctaccg cggcgccgac ctggcgcgct
      301 tcggccgcaa ggacgaggcc gcgttccgcg ccgccactgg cttcgtgttc caggacgccg
      361 cgctctgggc cgaccagaac atcctgatga acgtgacgat gccgctgcgc gtgcacaagg
      421 cctggctcgg cccgagcgcg atggccgagc tggcgcgcga gacgctcggc aggctcggct
      481 acgacgaggg catggcctac cggccggcgg agctgtcggc cggcgagcag aagctcgtct
      541 ccatcgcgcg cgccgtgatc cacgagccgc agatcgtgct catggacgac ccgacggcca
      601 gcctggacga ggacgcggcc gagcgggtct acgccctgat agccaaactg cgcgaacggc
      661 ggcgcacggt cgtgatcgtg gccaacagct ccgaactggc ctaccgcttc gccgaccggc
      721 tcggtatcat caagggcggc aggctggtag ccttcggcgc ctacgaggag acggtcggcg
      781 ccgcggaggc ggcactatcg ggctcgatag ccaggctcaa ggcgcgaggc gagcgcgtgg
      841 cggagccggg acgggaacgc gggccggcgc cgggcctgga cgccggatcg gagaattctc
      901 aagcggggga cgtgtaaacg atgaaattca agatcaagtt cgccgaccag atcgtaggcc
      961 tgttcatcat cgtcgcgctg gccttcttcg cggtcatcgt cgtactgatc ggcgccaacc
     1021 agcgctggtt cgccaaagac taccgcttcg tgacctcctt cccgtcagcc gccggcgtgg
     1081 ctccgggcac gtcgatactc atgaagggct tcacgatagg caagatcgac aagctgaggc
     1141 tgaacgccga caacgcggtg gacgccgact tccatatcta cgacacctac tacgacaagg
     1201 tacgcgagta ttcgatactc gagctgacgg tgtccccgat cggcctcggc tcccagctcc
     1261 tgttccaccc aggcacgggc gaggcccggc tcgaggaggg cgccttcgtg ccgacggccg
     1321 actcgatcca gggccagtcg ctgatcgacc aggagttggt cgtcataccg cccaaggacg
     1381 acacgatcac ccggctcctg gccaacgtga acccgctcct cgagaacacc aacaagacca
     1441 tcgtcaccgt caaccgcacc ctgaccgagg tcaaccgggc cctcgccggg cagagctccg
     1501 gcccgctcgg cgacatcgtc ggcgacgtca gcgtcgccgc ggccgccctg cccggcgcga
     1561 tggccggcgt cggcggcatc gtcggcgacg tcgagtcccg ggtcgcgata ctcctggacc
     1621 aggccggctc cctgctcgcc gagctcgaga ccatagccgg
//
LOCUS       DAOPHV010000616         1659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_105657_length_1659_cov_3.421446, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000616 DAOPHV010000000
VERSION     DAOPHV010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105657_length_1659_cov_3.421446"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLQ29_12665"
     CDS             complement(<1..238)
                     /locus_tag="PLQ29_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87542.1"
                     /translation="MKDAPSAALLVVDVQNYYLNPGSPFYRYSERCWPGCMAYIGRRV
                     ETSVFPAVTRLRQAFSAVGWPTVYLRLCASETDRS"
     gene            complement(243..1514)
                     /locus_tag="PLQ29_12670"
     CDS             complement(243..1514)
                     /locus_tag="PLQ29_12670"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HPG87543.1"
                     /translation="MHKVLSIVLGGGKGTRLFPLTKDRAKPAVPFGGRYRIVDIPISN
                     CINAGFKQIYILTQFNSASLHLHIAKTYVFDNFSNGFAEILAAEQTLTHSGWYEGTAD
                     AVRKNLVHFRTQQPSHYLIVSGDQLYRMDLADMFQRHLESGKALTKACTTVSREDASD
                     FGIMKIDADGRIVEFQEKPGPVKDISDYKIPKSMLKERRGSKEYLASMGIYIFDAGLL
                     ESALENDSNDFGKEIIPEILASRPVNSYIFDGYWEDIGTVRSFYEANLNLTDIAPEFN
                     FYDETSPIYTHRRNLPSSKINYSTMNQAITSDGCIITNASISRSIVGIRTIIETGANF
                     DGVVCMGADYYETEAQKRENAERGVPNIGIGRGSIIKGAIIDKNARIGADCRIGIDAR
                     DRADGEYGDYYIVDGVIVIPKNSVVMPGSII"
BASE COUNT          307 a          464 c          517 g          371 t
ORIGIN      
        1 cgctgcggtc cgtctccgag gcgcatagcc gcaggtagac ggtgggccag ccgacggcgg
       61 agaaggcctg tctgagcctg gttacggcgg ggaacaccga cgtctcaacg cgccggccta
      121 tgtacgccat gcaaccgggc cagcatcgct cggagtagcg gtagaacggc gagccggggt
      181 tgaggtaata gttctgcacg tcgacgacga gcagggcggc ggatggagca tccttcatgt
      241 gtctagatga tgctacccgg catgaccacc gagttcttgg ggattacgat gaccccgtcc
      301 acgatgtagt agtcgccgta ctcgccgtcg gcgcgatccc tcgcgtcgat gccgatgcgg
      361 cagtcggcgc cgatcctcgc gtttttatcg atgatggcgc ccttgatgat cgatccgcgg
      421 cctatgccta tattcggcac gccgcgctcg gcgttctccc gcttctgggc ctcggtctcg
      481 tagtaatcgg cgcccatgca gaccacgcca tcgaagttcg ctcccgtctc gatgatggtg
      541 cggattccga cgatcgacct cgagatcgac gcgttcgtga tgatgcagcc gtcggaggtg
      601 atggcctggt tcatcgtgct gtagttgatc ttcgacgaag gcaggttgcg gcgatgggtg
      661 tagatcgggc tcgtctcatc gtagaagttg aattctggcg cgatgtcggt caggttgaga
      721 ttggcctcgt agaaggaacg cacggtaccg atatcctccc agtacccgtc gaagatatac
      781 gagttgaccg ggcggctggc caggatctcg ggaattatct ccttaccgaa atcgttcgaa
      841 tcgttctcca gggcagattc gagcagaccg gcgtcgaaga tatagatacc catcgaagcc
      901 aggtactcct tcgatcctcg ccgttccttg agcatggatt tcggaatctt gtagtcggag
      961 atgtctttga ccggtcccgg cttctcctgg aattcgacta tgcggccatc agcgtcgatt
     1021 ttcatgatgc cgaagtcgct ggcgtcctcc cgtgagacgg tggtgcaggc tttggtcagg
     1081 gccttgcctg attccaggtg cctctggaac atgtccgcga ggtccatgcg gtagagctgg
     1141 tcgcccgaca cgatcaggta atgggaaggt tgctgggtcc tgaaatggac caggttctta
     1201 cggacggcat cggcggtgcc ctcgtaccag ccggaatggg tgagcgtctg ctcggcggcg
     1261 agaatctcgg cgaagccgtt cgagaagttg tcgaagacgt aggtcttggc gatgtgcagg
     1321 tggagggagg ccgagttgaa ctgcgtcagg atgtagatct gcttgaagcc ggcgttgatg
     1381 cagttcgata tggggatgtc gacgatcctg tagcggcctc cgaacggtac cgccggcttg
     1441 gccctatcct tggtaagcgg gaatagcctc gtacccttgc cacccccgag cacgatcgac
     1501 agcaccttat gcatcgctct cctccgttct attttttagc ccttcgcgcc gcttcagtat
     1561 agcacgccaa cggcggcggt ggcatcgatt taccaaactc gccgtatacc gtatcatagt
     1621 atagccatga tcgaagaaga cgccatagac cccgtcgat
//
LOCUS       DAOPHV010000617         1657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_105845_length_1657_cov_4.961923, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000617 DAOPHV010000000
VERSION     DAOPHV010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105845_length_1657_cov_4.961923"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..716)
                     /locus_tag="PLQ29_12675"
     CDS             complement(291..716)
                     /locus_tag="PLQ29_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87544.1"
                     /translation="MNARVVYYSATGNTRKVADAIAGAVGLEASTIDAYADAPVDLLF
                     LGAAVYATHDHGLHAGMKAFIAALDPAKVGRVALFSTGFVHSEAIAMMRALLERRGIP
                     VAEKSFFCLGRFALFNLGRPNAKDLAAAADFARGLIARP"
     gene            complement(838..1281)
                     /locus_tag="PLQ29_12680"
     CDS             complement(838..1281)
                     /locus_tag="PLQ29_12680"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPG87545.1"
                     /translation="MDDGLVYRKLNVGDIDSVVRMETDFRPGFIVQENAERFLRDPMN
                     WLIACLSGDRIVGFAYGYELYRLNGIGNMVYIHEVGVLPEYQRQGIGTELLQTIRRLC
                     KSTGICRFFLFTERSNTGACSLYRKMKGEEAHTDDVAYFFSDLDE"
     gene            1474..>1657
                     /locus_tag="PLQ29_12685"
     CDS             1474..>1657
                     /locus_tag="PLQ29_12685"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HPG87546.1"
                     /translation="MERIDAIAFTDPSAVPDDAQLERVLGASYEAYRGLLGVFEKRGL
                     TYEWRYYSDGKAWLCKV"
BASE COUNT          312 a          484 c          523 g          338 t
ORIGIN      
        1 cgtagttcaa gcgggctcgc tgcgctcgcc gcttaactac ggggcgctgc gttcatgcct
       61 gcgatgtcga acgccgacgc gtggcgcggc gttcgcttcc cgtagttcaa gcgggctcgc
      121 tgcgctcgcc gcttaactac ggggcgctgc gttcaggcct gcgatgtcga acgacggccg
      181 acgatcgccg cgatgagtcg gcgatcgcgt gcatgcgagg cctgggtacg agcaatgttc
      241 actgggcggc gcgtcgttgc agctagcggg tagggtatcg gtaagcgcta ctacggacgg
      301 gcgatcaggc cccgggcgaa gtcggcggcg gcggcgaggt ccttggcgtt cggtcggccc
      361 aggttgaaga gcgcgaagcg cccgaggcag aagaagctct tctcggccac tggtatgccg
      421 cgccgctcga gcagggcgcg catcatcgct atggcctcgc tatggacgaa gccggtcgag
      481 aacagcgcga ccctgccgac cttggccggg tccagagcag cgatgaacgc cttcatgccg
      541 gcgtgcaggc cgtggtcgtg cgtggcgtag acggccgcgc cgagaaagag caggtctacc
      601 ggcgcgtccg cgtacgcgtc gatcgtcgac gcctcgagcc cgacggctcc agcgatggcg
      661 tctgccacct tcctcgtgtt tccggtggcc gagtaataga cgacccgagc gttcatgcgg
      721 tagtcccttc cacggcgatg gagcgccgcg cccccagtct gcacgcgacg gccttttctt
      781 gcaagcggaa tagcacgacg cgccgcggcg ggcggtcgtc ggactcggtc cgtgaatcta
      841 ctcgtccagg tcgctgaaga aataggcgac gtcgtcagta tgggcttctt cgcctttcat
      901 ttttctatat aacgagcagg ccccggtatt cgatcgttca gtaaatagga agaacctgca
      961 tattcccgtc gatttgcaga gccttcgtat cgtttgtaat aattcggtgc cgattccttg
     1021 tctctgatat tcgggtaaga cgccgacctc atggatatag accatattcc cgataccgtt
     1081 caggcgataa agctcatacc catacgcgaa accgactatc ctatcgccgg acaggcaggc
     1141 gatcagccag ttcatgggat cccgcaggaa tcgttcggcg ttttcttgta cgatgaaccc
     1201 gggcctgaaa tcggtttcca tccggactac cgaatcgata tcgccgacgt tcagtttcct
     1261 gtaaacgagt ccgtcatcca taccggcttc cctcgaccta aggatgcgat catcatagcg
     1321 agtatgaagg aatagtcatc caggacgcgg atgcgggacg atggacccgg aaggtcgcgg
     1381 ctagcctcta caaggttcga ggaacagact atacactagc gggcatggat acggccgtga
     1441 tctctattcg ttatggatgc caaggaggcg gttatggagc gaatcgacgc catcgcgttt
     1501 actgatccgt cggctgttcc cgacgatgcc cagcttgaac gggttctcgg cgcgtcatac
     1561 gaggcgtacc gcggccttct tggagtattc gagaagcgcg gcctgacgta cgagtggcga
     1621 tactacagtg acgggaaggc ctggctgtgc aaggtgc
//
LOCUS       DAOPHV010000618         1657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_105858_length_1657_cov_3.677903, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000618 DAOPHV010000000
VERSION     DAOPHV010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105858_length_1657_cov_3.677903"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /locus_tag="PLQ29_12690"
     CDS             complement(<1..1348)
                     /locus_tag="PLQ29_12690"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA];
                     GO_process: GO:0008380 - RNA splicing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase"
                     /protein_id="HPG87547.1"
                     /translation="MDPKSLPVYQQKERILASLRDNQVIVVESPTGSGKTTQLPVILN
                     EAGYADHGVIGVTQPRRIAAISVSEFISRQLGTPLPGLVGYKMRFEDMTNQYTRIKIM
                     TDGILLQEMKLDPSLSRYSVIMVDEAHERSLNIDFILGLLKRVLEARPEFKVIVSSAT
                     INAEVFSAYFGDCPIVKIETQVYPVTVVWDPPAIEANPDSLIDKIEQIVDRIIGDRRE
                     GDILVFLPGEKLIKDCMERLAYGESRSRIHLVPLYARLGKDEQARVFDKAPLGKKKVI
                     ISTNIAETSVTIDGVTSVIDSGQAKLNFYNPRTYTASLVQVPVSKASSNQRKGRAGRT
                     APGYCYRLYPREDFENRSLFTMEEIYRTDLSEVVLRMAELGITDFERFDFISKPPRAG
                     VLGAMDVLNLLEAFEPDRSLSKVGQMMCAFPLLPRLSRMIVEAILRYPDVVEEIVIV"
     gene            1414..>1657
                     /locus_tag="PLQ29_12695"
     CDS             1414..>1657
                     /locus_tag="PLQ29_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87548.1"
                     /translation="MIKEILSVLVVVAAMAAIAAGRVPGLRMNRAAIAFAAAALLVAI
                     GAIPLKDAFAAIDLGTIVLLLSMMVLVAAIRLSGLFD"
BASE COUNT          274 a          492 c          541 g          350 t
ORIGIN      
        1 cgacgatgac gatctcctcg acgacgtcgg ggtagcggag tatggcctcg acaatcatgc
       61 gcgacagcct gggcaggagc gggaaggcac acatcatctg gccgaccttg gacaggctcc
      121 ggtccggctc gaaggcctcg agcaggttga gcacgtccat cgcgcccagg acaccggccc
      181 gcggcggttt ggagatgaag tcgaagcgct cgaagtcggt gatgcccagc tcggccatgc
      241 gtagcaccac ttcggagagg tcggtgcggt agatctcttc catcgtgaac agcgatcggt
      301 tctcgaagtc ctcgcgggga tagaggcggt agcagtagcc gggggcggta cggcccgccc
      361 tgcccttgcg ctggttactc gacgccttgg acaccggcac ctggaccagg ctggcggtat
      421 aggtcctggg attgtagaaa ttgagcttgg cctggcccga gtcgatgacg ctcgtgacgc
      481 cgtcgatggt cacgctggtc tcggcgatgt tcgtcgagat gatcaccttc ttcttgccta
      541 gcggcgcctt gtcgaacacg cgcgcctgct cgtccttgcc gagcctggcg tagagcggca
      601 ccaggtggat gcgcgatcgg ctttcgccgt aggcgaggcg ctccatgcag tccttgatca
      661 gtttttcacc gggcaggaac acgaggatgt ccccttcgcg cctgtccccg atgatccggt
      721 cgacgatctg ctctatcttg tcgatcagcg agtcggggtt ggcttcgatg gccggcggat
      781 cccagacgac ggttaccgga tagacctggg tctctatctt gacgatgggg cagtccccga
      841 agtaggcgct gaagacctcg gcgttgatcg tggcgctcga gactatcacc ttgaactccg
      901 gcctcgcctc gagcacccgt ttgaggagcc cgaggatgaa gtcgatgttc aggctgcgct
      961 cgtgcgcctc gtcgaccatg atcaccgagt acctggacag cgacgggtcg agcttcatct
     1021 cctggagcag gatgccgtcg gtcattatct ttattctggt gtattggttg gtcatgtcct
     1081 cgaagcgcat cttgtagccg acgaggcctg gtagcggcgt cccgagctgt ctggagatga
     1141 actcgctgac cgagatggcg gcgatgcgtc gcggctgggt cacgccgatg acgccgtggt
     1201 cggcgtagcc ggcttcgttc agtatgaccg gaagctgggt ggtcttgccc gagccggtcg
     1261 ggctctcgac gacgatgacc tggttgtcgc gcagcgaggc gagaatacgc tctttctgct
     1321 gatagactgg taggctttta ggatccatag gatccattgt aatccaaagg gaccgattat
     1381 caaaggaccg gcggtcgctg tatactccgc cgcatgatca aggaaatcct gtcggtactc
     1441 gttgtcgtcg cggcgatggc cgcgatagcc gccggccgcg tgccggggct gcgcatgaat
     1501 cgcgctgcca tcgcgttcgc ggcagcggcg ctgctcgtcg ccatcggggc catcccgctc
     1561 aaggacgcct tcgcggccat agacctaggc accatagttc tcctcctctc gatgatggtg
     1621 cttgtcgcag ccatccgcct ctcgggcttg ttcgacg
//
LOCUS       DAOPHV010000619         1656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_105978_length_1656_cov_3.156777, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000619 DAOPHV010000000
VERSION     DAOPHV010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105978_length_1656_cov_3.156777"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..>1656
                     /locus_tag="PLQ29_12700"
     CDS             52..>1656
                     /locus_tag="PLQ29_12700"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPG87549.1"
                     /translation="MIRRVPTFCGKDCGGNACPLLAVLNGGRVERLERNPAGAELKPC
                     PRGYALHREHYAAERLLSPLIADGPRGCGRYREASWDEALSLVADRLGELRARHGPTG
                     LMALGSSGSTGLVHDSQNLLKRFVNACGGGVHPRSNYSNGAAGFALPYLFGAAASESG
                     WDAKTVRQSRLIVLWGANILEARLGAELGPEVAAAARSGVPVVYIDPRRTMTAKALGA
                     SWIPIRPGTDAAMMLATLRVLFRDGLVDRDGARRLAVGLDELERYVSGDDDGVERSPA
                     WAERICGVPAGVIEGFARSYAAARPAMLVPGYSIQRVARGEETFRLSVALQVATGNLG
                     VPGGSSGSLNNRLPGVRTGAMPDLADRLVGDARTLTVPVLRWPDAILSGRSGGFPGDV
                     KAAYVAGFNALGQGADNRKSARALESLEFSVCHEMFMTPTARRCDVVLPVLSPLEKED
                     ACAPWHGNYLLYKPAALPPRGLARSDYDIFAELSERLGAGEAFSEGLSATGWLDRLLA
                     ESEIRDLEAFKATGVYFGADRERVGLA"
BASE COUNT          246 a          593 c          575 g          242 t
ORIGIN      
        1 cggcttgact gcggcttgac tgcgggtcgc cgtacgtcgt ctactcaggg catgatacgg
       61 cgagtaccga ctttctgcgg caaggactgc ggcggcaacg cctgcccgct cctggcggtc
      121 ttgaacggcg gccgcgtcga gcgcctcgag aggaaccccg ccggagccga gctcaagcca
      181 tgcccgcgag gctacgcgct ccaccgagag cactacgcgg cagaacggct cctctcgccg
      241 ctcatcgccg acggcccgcg cggctgtggt cgctaccggg aggcgagctg ggacgaggcg
      301 ctgtcgctgg tcgccgatcg cctcggcgag ctccgggcca gacatgggcc gaccggcctg
      361 atggcgctcg gtagctccgg ctcgacgggc ctcgtccacg acagccagaa cctgctcaag
      421 cgcttcgtga acgcctgcgg cggcggcgtc catccgagga gcaactattc aaacggggcg
      481 gctggcttcg cgctaccgta cctattcgga gccgcggcca gcgaatccgg ctgggacgcg
      541 aagacggtca ggcaaagccg cctgatcgtc ctttggggcg cgaacatact cgaggccagg
      601 ctcggcgcgg agctcgggcc cgaggtcgcc gcagccgccc gatcgggcgt gccggtcgtc
      661 tatatcgacc cgcgcaggac gatgacggcc aaagccctcg gggcgagctg gataccgata
      721 cggccgggca ccgatgcggc catgatgctc gcgacgctgc gcgtcctatt ccgggacggt
      781 ctcgtcgacc gcgatggcgc ccgccgcctg gcggtcgggc tcgacgagct ggaacgctac
      841 gtctcaggag acgatgacgg cgtcgagcgc tcgcccgcct gggccgagcg tatctgcggc
      901 gtgccggccg gcgtcatcga gggcttcgcc cgtagctacg cggccgcccg cccagccatg
      961 ctcgtccccg gctactcgat ccagcgcgtc gcccgcggcg aggagacctt caggctatcg
     1021 gtcgccctgc aggtcgcgac cggcaacctc ggcgtcccgg gaggctcgtc gggctcgctc
     1081 aacaatcgcc tgcccggcgt gcgcacggga gcgatgccgg acctggccga ccgcctagtc
     1141 ggcgacgccc gaacccttac cgtgccggtg ctacgctggc ccgacgccat cctgtccggc
     1201 cgctcgggcg gattcccggg ggacgtcaag gccgcctacg tggccggctt caacgcgctg
     1261 ggccagggcg ccgacaaccg aaaatcggcc cgagcgctcg agtcgctcga gttctcggtc
     1321 tgtcacgaga tgttcatgac tccgacggcc aggcgatgcg acgtggtact accggtcctc
     1381 tcgccgctcg agaaggaaga cgcctgcgcg ccctggcacg gcaactacct gctgtacaaa
     1441 cccgccgcgc tgccgccgcg gggcctggcc aggagcgact acgacatctt cgcggagctg
     1501 tcggagcgtc tcggcgcagg cgaagccttc tcggagggcc tgagcgctac gggctggctc
     1561 gaccggctcc tagccgaatc agaaatccgc gacctcgagg cgttcaaggc gacaggcgtc
     1621 tacttcggcg ccgaccgcga gcgcgtgggg ctggcc
//
LOCUS       DAOPHV010000620         1652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_106316_length_1652_cov_5.147151, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000620 DAOPHV010000000
VERSION     DAOPHV010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106316_length_1652_cov_5.147151"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="PLQ29_12705"
     CDS             complement(<1..1020)
                     /locus_tag="PLQ29_12705"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPG87550.1"
                     /translation="MEILARSSYIRRKDMDSVLNCLVTDRLGPGVFADRFIKVAKERL
                     GFEYAVAVRSPIVALRLSFECLGVGSGDRVGLSALSPSWAYKAVSSLGATPVWLDVDD
                     SSASLSAKAYDRLGEASVKALYLAEPWGIMPDPAPLAELGVPIVEDASYSIGAKAGEA
                     KAGFLGTYGIISLEPASSITAGGGALLYSPGRREAQALRNASEGLPPEELMNDMNAAL
                     AASQLKDLERFIEKRRELADLYAQSLARAHRKPLAPTIECEPSNFGCVVVLDSGVKDV
                     RAYARKKDVDTAMAFDDTCVAAGFVPDGACPIAASLANRSVAFPMNQRVGKSAAQKIA
                     KVLATL"
     gene            complement(1033..1512)
                     /locus_tag="PLQ29_12710"
     CDS             complement(1033..1512)
                     /locus_tag="PLQ29_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPG87551.1"
                     /translation="MVETEALLQLVTFQLSEELYGIDIMDVKEIVTLQEIRTIPNAPS
                     FVEGLYNLRGEIIPIINLHKRFHLRKAQLGEDETLLSGFIIIDLGGMRIGVIIDKVER
                     VISIDYKEIQPPPQMISGIGAEYINGVVNQKNGYLIILDIRKLFSAKELQKIEEIRK"
BASE COUNT          329 a          493 c          477 g          353 t
ORIGIN      
        1 cagggtagcg agcaccttgg cgatcttctg cgcggcgctc ttgccgacgc gctggttcat
       61 cgggaaggcg accgatcggt tcgccagcga cgcggcgatg gggcaggctc catcggggac
      121 gaagccagcc gcgacgcagg tatcgtcgaa ggccatcgcg gtatcgacgt ctttcttcct
      181 ggcgtaggcg cgcacgtcct tgacccctga gtcgagtacc acgacgcagc caaaattcga
      241 cggctcgcat tcgatagtcg gtgcgagcgg cttacgatga gcgcgtgcca gtgactgggc
      301 gtatagatcg gccagctccc gccgcttctc gatgaaccgt tccaggtcct tgagctgcga
      361 ggccgccaac gccgcgttca tgtcgttcat gagctcctcg ggcggcaagc cctcggacgc
      421 gttccggagt gcctgggcct cgcgcctgcc gggggagtac aacaacgcgc ctcctccggc
      481 agtgatcgac gaggccggtt cgaggctgat gatgccgtag gtaccgagga atccggcttt
      541 cgcctcgccg gccttggcgc caatcgagta gctggcgtcc tcgacgatgg gtacgccgag
      601 ttccgccagc ggggctggat ccggcatgat gccccagggc tcggccaggt acagcgcctt
      661 gacggaggct tcgccgaggc ggtcgtaggc cttcgccgag agcgacgcgc tcgagtcgtc
      721 gacatccagc cagaccggag tcgctccgag cgacgatacg gccttatacg cccaggaggg
      781 agagagcgct gacagaccga ccctgtcgcc agagccaacg ccaaggcatt cgaacgatag
      841 gcgtagggca acgattgggc tcctgacggc tacggcgtac tcgaagccta agcgctcctt
      901 ggctaccttg ataaaacgat ccgcgaacac tccggggcca agccggtcgg tgacgagaca
      961 attcaagacc gaatccatat cttttctgcg gatatatgag ctacgcgcta aaatttccat
     1021 ggacaggaaa gcctacttcc tgatctcttc gatcttctgc agttccttgg cgctgaacag
     1081 cttgcgaata tcgagaatta tgagatagcc gttcttttgg ttgacgacgc cgtttatata
     1141 ctccgccccg atccctgaga tcatttgcgg cggcggctgt atttccttgt aatctataga
     1201 gataacacgt tcaaccttgt cgattataac acctattctc attccgccga gatcgataat
     1261 gatgaagccg gacagcaggg tctcatcctc gccaagctgc gccttcctta agtgaaatcg
     1321 cttatgcaga ttgatgatgg gaattatttc gccacgcaga ttatacagac cctcgacgaa
     1381 cgacggggcg ttcgggatgg ttcttatctc ctgaagcgtt acaatttcct tgacgtccat
     1441 tatatcgata ccatagagct cttcgctgag ctggaatgta acgagctgca atagagcttc
     1501 agtctcgacc atgcggccct ccggcttcgt tttcatagga tgcacgtcgg caaggatagc
     1561 atctttcgaa ccggcaagca agggttcggc ttttgcgccg aacgccccgc gccctcccgt
     1621 cgcgtgaccc tatcgagtct tcaatgcacc tt
//
LOCUS       DAOPHV010000621         1650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_106544_length_1650_cov_2.957367, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000621 DAOPHV010000000
VERSION     DAOPHV010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106544_length_1650_cov_2.957367"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..863
                     /locus_tag="PLQ29_12715"
     CDS             291..863
                     /locus_tag="PLQ29_12715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPG87552.1"
                     /translation="MRGGDDAALAFEALWDAYYRRLMVFAAAYRGLPSAERHDAAADA
                     LIAAFGGLRGFDPRRPLSPWVYRIAANHFSDAARRARRESTIEVGQADSAGSDDGATG
                     GYRGPAVVEPATSGDHEADSVERDLVERCGAAIAALPEADRRVAMLRFYEGFSAADVG
                     RALGMPAGTVRWRVGVIRAAIRAATGEDRP"
     gene            860..1192
                     /locus_tag="PLQ29_12720"
     CDS             860..1192
                     /locus_tag="PLQ29_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87553.1"
                     /translation="MTADERVRAAFESEVRRVEAREPSSVETRRALAAARLMPISATR
                     RRLGAGELIPVVAMAAAAFAVAAIDLGSMQRLRPLAGVLAAAVPDSAGERFVDFVLEA
                     GESYRSAN"
     gene            1224..>1650
                     /locus_tag="PLQ29_12725"
     CDS             1224..>1650
                     /locus_tag="PLQ29_12725"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPG87554.1"
                     /translation="MLKRIVFPMLVVVAVIALTMFVCAVTPWMYIDIASFILVPIGSL
                     LFMGLAFGWKASGAAFSSAWRPNASRRELEASVRFFKGLRAVVWSFGALGAMIGLIAV
                     LSNLTDRARVGPNLAVALVSVLYAAIFDLLAALPFLSVST"
BASE COUNT          248 a          482 c          607 g          313 t
ORIGIN      
        1 ggtacgggtg aagtcagccg tagggaagcg tactcgccgc tggggcggct cgtcgcttcg
       61 tggtacgggt gaagtcagcc gtggggacgc gtactcgccg cttcgtggta cgggcggcgg
      121 caaccggaag ataaattccg ccaacaccga ggcggttcgg ggcgtacaat aagcgtatgc
      181 aagatagtga cggcgcagaa gaaggcgcta gcgagcgttg tacgactgct ggggctcagc
      241 gagctcgagc ggcgtgagag ccgggcccgg cggagcttcg agacgcggcc atgaggggtg
      301 gggacgatgc ggcgctggcc ttcgaggcgc tgtgggacgc ctattatagg cggctgatgg
      361 tcttcgccgc cgcttaccgg ggacttccta gcgccgaacg gcacgacgcg gccgcggacg
      421 cgttgatcgc cgcgtttggg ggcctacgag gcttcgaccc gaggaggcct ttgtcgccgt
      481 gggtataccg catcgcggcc aatcacttct cggacgcggc caggcgggct cgacgagagt
      541 cgacgataga ggtcgggcag gctgattcgg ccggttccga tgatggcgct acagggggct
      601 atcgaggtcc ggccgtagtc gagcccgcga cgagcggaga ccatgaggcg gactctgtcg
      661 agcgtgacct ggtcgagcgc tgcggagccg ctatagcggc gttgcccgag gcggatcgga
      721 gagtcgccat gctacgcttc tacgaaggct tcagcgccgc cgacgtcggc agggcgctcg
      781 gtatgccggc cggtacggtg cgttggcgcg tcggcgttat tcgcgcggcc atccgcgcgg
      841 ctacggggga ggataggcca tgacggcgga cgaacgggta cgggcggctt tcgagtcgga
      901 agtgcgccga gtcgaggctc gggagccttc gagcgtcgag acgaggcggg ccttggccgc
      961 ggccaggctt atgcctatct cggcgacgcg acgacggcta ggcgcgggcg agctgatacc
     1021 ggtcgtcgcg atggccgctg cggctttcgc cgtggcggcc atcgatcttg gttcgatgca
     1081 gcgcctgcgc cccctcgccg gggtgcttgc cgccgcggtg cctgacagcg ccggggaacg
     1141 tttcgtggat ttcgtactcg aggccggcga gtcgtatcgg tcagcaaact agtcatcttc
     1201 aacgcaaatt ccaggaggaa agagtgctca agcgaatcgt gttcccgatg ctcgtcgtcg
     1261 tcgccgtgat cgcgctcacc atgtttgtgt gcgcggtgac gccttggatg tatatcgaca
     1321 tcgcgtcgtt catcctcgtg cctatcggct ccctgctctt catggggctg gctttcggct
     1381 ggaaggcgag cggggcggct ttctcgtcag cttggagacc caacgcctcg aggcgcgagc
     1441 tcgaggcatc ggttaggttc ttcaaggggc tacgcgcggt cgtctggtcc ttcggggctc
     1501 tcggcgctat gatcggcctc atcgccgtat tgagcaatct gacggatagg gcgagggtgg
     1561 ggccgaacct ggccgtggcc ctcgtctcgg tattgtacgc ggccatcttc gacctgctcg
     1621 ctgccttgcc cttcctatcc gtatcgacga
//
LOCUS       DAOPHV010000622         1649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_106629_length_1649_cov_3.656211, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000622 DAOPHV010000000
VERSION     DAOPHV010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106629_length_1649_cov_3.656211"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PLQ29_12730"
     CDS             <1..379
                     /locus_tag="PLQ29_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG87555.1"
                     /translation="ALDDLLEEFGISSLRRQYAYTLSGGERRRTEIARALAIRPKFLL
                     LDEPFAGIDPIAVHEIKSIVRRLSDKGIGVLITDHNVRDTLEITHEAHIINKGEILVS
                     GDRDAILESELARQIYLGETFRM"
     gene            505..1278
                     /locus_tag="PLQ29_12735"
     CDS             505..1278
                     /locus_tag="PLQ29_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPG87556.1"
                     /translation="MRYFDTHAHIGLIFDDPIEQLLACQQAKQYGVQRIVSICNSLMD
                     FFQVYDSLKTSEHVYHAAGVSPSEVASPGRDWRAKLDEALKLPRVIAVGEIGLDYYHR
                     YGDKDSQIELFIDQLDIAAKHDLPVIIHNREAGKDVLDVLRDRLPPAGGVLHCYSENA
                     EYALRALDLNLYFSFAGNLTYRNAKNLHETVRALPLDRILVESEAPFMVPAKYRGKRN
                     KPEYLPATVDDLAMLLDLPVEDVAEATYQNACRLFRVPE"
     gene            1373..>1649
                     /locus_tag="PLQ29_12740"
     CDS             1373..>1649
                     /locus_tag="PLQ29_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HPG87557.1"
                     /translation="MNLHVPVRVGSYTTRGNLFLAPVAGYSDAAFRSICADFGCDLAF
                     TEMVSSEALTRGHLKTEVLLAREDNEPDYAVQLFGSGPETMAAAALIV"
BASE COUNT          317 a          542 c          488 g          302 t
ORIGIN      
        1 ggccctcgac gacctgctcg aggaatttgg catatcgtcg ctacgcagac aatacgccta
       61 taccctctcg ggcggcgaac gccgacgtac cgagatagca cgcgcgctgg cgatacggcc
      121 caagttcctg ctgctcgacg agcccttcgc cggcatcgac ccgattgccg tgcacgagat
      181 caagagcatc gtccgtaggc tgtcggacaa gggcatcggc gtgctgatca ccgaccacaa
      241 cgtgcgcgat accctggaga taacccacga ggcccatatc atcaacaagg gtgagattct
      301 cgtctccgga gaccgggacg cgatacttga atcggagctg gctaggcaga tatacttggg
      361 tgagacgttc cgaatgtagc ctcctcatgc cgagggacgg agtcccggta gcttatcgtg
      421 ctaaacttga cggcacggta ggctcgtgtt acattgaggt acccggttac gggctccctc
      481 atcactcaac ccaatggaag acagatgcga tactttgaca ctcatgccca catcgggctt
      541 atcttcgatg atcctatcga gcagctgctc gcctgccagc aggccaagca gtacggcgtc
      601 cagcggatcg tcagcatatg caacagcctc atggatttct tccaggtgta cgatagcctg
      661 aagacgtcgg agcacgtgta ccacgccgcc ggcgtgtcgc cgtccgaggt cgcctcgccc
      721 ggccgcgact ggcgcgccaa gctcgacgag gccctcaagc taccccgtgt cattgccgtg
      781 ggcgagatcg gcctggacta ctatcatcgc tacggcgaca aggatagcca gatcgagctg
      841 ttcatcgacc agctggatat agcggccaag cacgatctgc cggtcatcat tcataaccgc
      901 gaggccggca aggacgtgct cgacgtgcta cgcgaccgcc tgcccccggc tggaggcgtg
      961 ctccactgtt attccgagaa cgccgagtac gcgctacgag ccctcgatct gaacctgtac
     1021 ttctcgttcg cgggcaacct gacctaccgg aacgccaaga acctgcacga gaccgtgcgc
     1081 gccctgccgc tcgaccgcat cctggtcgag tccgaggcgc cgttcatggt gccggccaag
     1141 taccgcggta agcgcaacaa acccgagtac ctgcccgcca cggtcgacga cctggccatg
     1201 ctcctcgact tgccggtcga ggatgtggcc gaggcgacct accagaacgc ctgcaggctg
     1261 ttccgcgtgc ccgaatgata ccgtgggccg gccgtacgcg ccgcgccgcg acgataggag
     1321 gcgccccgtg gtacgggcgc ctttttttag ctacattctt cccggagaca ccatgaacct
     1381 gcacgtacct gtccgcgtcg ggtcgtatac gacgagaggc aacctgttcc tggccccggt
     1441 ggccggatac tccgacgcgg cgttccgttc gatctgcgct gacttcggtt gcgacctggc
     1501 ctttaccgag atggtcagtt ccgaggccct gacccgcggc catctcaaga ccgaggtcct
     1561 gctggcgagg gaggacaacg aaccggacta cgccgtccag ctgttcggct cggggcctga
     1621 gacgatggcg gcggccgccc tcatcgtcg
//
LOCUS       DAOPHV010000623         1643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_107180_length_1643_cov_3.620277, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000623 DAOPHV010000000
VERSION     DAOPHV010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107180_length_1643_cov_3.620277"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PLQ29_12745"
     CDS             <1..404
                     /locus_tag="PLQ29_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87558.1"
                     /translation="QRRQRRAGLGRAADQRRIQQLNSVIQENASSSEELASTAEEISA
                     QTMAMRDEVAFLKTGKRNLGAYDSPRPRTAAHTAGSRLGSGVAVKRPATDAGEGPSHV
                     APKAQDKRPHAGNTAITIAHADTEDDEFERF"
     gene            407..967
                     /locus_tag="PLQ29_12750"
     CDS             407..967
                     /locus_tag="PLQ29_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPG87559.1"
                     /translation="MTSTIDTNPAAAPDTARGGATGQYLLFELAGEHYAFDVVRAKEV
                     LEDVSITPLPTKAPFLSGVINLRGSIIPIIDLRTKFEISGRGGRGSVIVIEIGDKDRQ
                     AIVGALVDSVKGVARFGPDELEAPPKFGMRVESALVKAIAKQSGLFVVILDSELLFSE
                     RHLWSDGPAGIQAGTPESTTSGIRVG"
     gene            969..>1643
                     /locus_tag="PLQ29_12755"
     CDS             969..>1643
                     /locus_tag="PLQ29_12755"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HPG87560.1"
                     /translation="MHDRSSIAPLSDSEFRKIASFIEQEFGIRMPEAKKALLQGRLHK
                     RLVACGKRSYGEYFGFVTEDPAGKDEFLVFTDLVSTHETSFFREPGHFKHLRQILPDL
                     AAERSRLRFLCAACSTGEEAYSLAMTIDETLSEAGLGRVPFTIEGFDLSSHAVEAARR
                     GVFSEARAKKIPAEMTKRYMMRSKNPGKEVRRAVPELRARMLFHTGNLLDDMGLAERR
                     YDVIFCR"
BASE COUNT          328 a          532 c          527 g          256 t
ORIGIN      
        1 atcaacgccg ccagcggcga gcaggcctcg ggcgtgcagc agatcaacgc cgcatacagc
       61 agctcaactc ggtcatccag gagaacgcct cctcgtccga ggagctggcc tccaccgccg
      121 aggagatatc ggcccagacc atggccatgc gcgacgaggt ggcgttcctt aaaactggca
      181 aacgaaacct gggcgcctac gatagcccgc gccctcggac cgcggcccat accgccggga
      241 gccggctcgg ctcaggcgtc gccgtcaagc ggccggcgac ggacgccggc gaaggtccat
      301 cccacgtcgc gcctaaggcg caggataaga ggccgcacgc aggcaatacg gccatcacga
      361 tcgcccacgc cgatacagag gacgacgaat tcgaaaggtt ctgagcatga catcgacgat
      421 agacacgaac cccgccgcgg caccggacac cgccagggga ggggcgacgg ggcagtacct
      481 tttattcgaa ctggctggcg agcactacgc cttcgatgtg gtcagggcca aggaggtgct
      541 cgaggacgtc tcgatcacgc cgctaccgac caaggcgccc ttcctgtcgg gcgtgatcaa
      601 cctccgcggc agcatcatcc ccatcatcga cctgcggacg aaattcgaaa taagcggacg
      661 cggcggccga ggttcggtca tcgtcattga aataggggac aaggacaggc aggccatagt
      721 cggcgccctt gtcgactcgg tcaaaggcgt cgctcgcttc ggcccggatg agctcgaggc
      781 gccgcccaag ttcggcatgc gcgtcgagtc cgcgctcgtc aaggcgatag ccaagcagtc
      841 ggggctcttc gtcgtcatcc tggattccga gttactcttc tcggagcgcc acctctggag
      901 cgacggtccc gctggaatcc aggccgggac acccgagtcg acgacatccg ggatccgagt
      961 aggctgagat gcacgaccga tcgagcatag cgccgctgtc cgatagcgag ttcaggaaga
     1021 tagcgtcgtt catcgaacag gagttcggta tacgtatgcc ggaggccaag aaggcgctcc
     1081 tgcagggccg cctgcacaag cggctcgtgg cctgcggcaa gcgtagctac ggcgagtact
     1141 tcggcttcgt gaccgaggac ccggccggca aggacgaatt tctggtcttt accgacttgg
     1201 tttcaaccca cgagaccagc ttcttcaggg agcccggcca tttcaagcac ctcaggcaga
     1261 tcctgccgga cttagccgcc gagcgctcgc gtctacgctt cctatgcgcg gcctgctcca
     1321 cgggagagga agcctatagc ctcgcgatga ccatcgacga gacgctgtcg gaggcggggc
     1381 tcggtcgcgt accgttcacg atcgagggct tcgacctgtc gtcgcacgcg gtcgaggccg
     1441 cgaggcgagg cgtcttctcc gaggcgcgcg ccaagaagat cccggccgag atgacgaaac
     1501 ggtacatgat gcgtagcaag aacccgggca aggaggtccg ccgcgccgtg cccgagctgc
     1561 gcgcgcgcat gctgttccat accggcaacc tgctcgacga catgggcctg gcggaacgtc
     1621 ggtacgacgt gatcttctgc agg
//
LOCUS       DAOPHV010000624         1641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_107395_length_1641_cov_2.870744, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000624 DAOPHV010000000
VERSION     DAOPHV010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107395_length_1641_cov_2.870744"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..>1641)
                     /locus_tag="PLQ29_12760"
     CDS             complement(128..>1641)
                     /locus_tag="PLQ29_12760"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPG87561.1"
                     /translation="EAAFCLELKTDDDRAGLPEFVLDAAKSVAADKGLGGYAINLSPS
                     LVDPFLTYSTRRDLREQVWRAFKARGESRAERDTKPVAEKILRLRAELAGLMGYASYA
                     DYALVDRMAKKPAAVDDLLMRVWKPARARALEEQKDLEAIARKEGFSGPIMGWDWNFY
                     AEKLRQERYALDEAELKPYFQLEKMTDAMFDAAGRLYGVAFKELTGVPLYHPDARLYE
                     VSDKASGAIVGVFITDSFARPTKRGGAWMNEFRNQSRNRSGGYPYPIVINNNNFAKAP
                     AGKPTLLSLDDVRTLFHEFGHGLHGLLSNVTYNRLAGTNVLRDFVELPSQINEHWALT
                     PEILKKHAVHAVSGKPISDELISLIKKSEHFNQGFLTVAYTSCALIDMSLHQHPNPDR
                     LDLAAFEKAECERLGVPEAVGMRHRLPQFRHLFADNGYAAGYYVYMWAEVLDADGFEA
                     FEASGDVFNADLAAKFKRFVLSAGNTLDPAEAYRAFRGRDPDVGALLRGRGLS"
BASE COUNT          249 a          540 c          562 g          290 t
ORIGIN      
        1 tcgaagtccg gccccctgct cgacgtaccg ttcgccgagc tcgagggacg ataggccgac
       61 gcggactgca actggtcgta cagagaacgg ggcccgcgac cgcctgagcg atcacgggcc
      121 ctgtatttca cgacagccct cgtccccgta gcagggcgcc cacgtcggga tcccgtcccc
      181 tgaaggcgcg gtacgcctcc gcggggtcga gcgtgttgcc ggccgacagc acgaagcgct
      241 tgaacttggc ggccaggtcg gcgttgaaca cgtcgccgga cgcctcgaag gcctcgaagc
      301 cgtccgcgtc gagcacctcg gcccacatgt atacgtaata gccggccgca tagccgttgt
      361 cggcgaacag gtgcctgaac tgcggcaagc ggtggcgcat gcccacggct tccggcaccc
      421 cgaggcgctc gcactcggcc ttctcgaagg cggccaggtc gagccggtcg gggttcgggt
      481 gctggtgtag cgacatgtcg atcagcgcgc acgaagtgta ggccaccgtc aggaagccct
      541 ggttgaagtg ctcgctcttc ttgatcagcg agatcagctc gtccgagatg ggcttgccgc
      601 ttaccgcgtg caccgcgtgc ttcttgagta tctccggcgt cagcgcccag tgctcgttga
      661 tctggctggg tagctcgacg aagtccctga gcacgttggt gccggcgagc cggttatagg
      721 tcacgttcga caggagcccg tgcaggccgt ggccgaactc gtggaacagc gtacgcacgt
      781 cgtcgagcga cagcagggtc ggcttgccgg ccggcgcctt ggcgaagttg ttgttattga
      841 tgacgatcgg gtatgggtag ccgccggagc ggttcctgga ctggttgcgg aactcgttca
      901 tccaggcgcc gccgcgcttc gtcggcctgg cgaaggagtc ggtgatgaac acgccgacga
      961 tggcgccgga ggccttgtcg ctcacctcgt agagcctggc gtcgggatgg tagagcggca
     1021 cgcccgtaag ctccttgaac gccacgccgt agagcctgcc ggccgcgtcg aacatcgcgt
     1081 cggtcatctt ctcgagctgg aagtacggct tgagctccgc ctcgtcgagc gcgtagcgct
     1141 cctgcctgag cttctcggcg tagaagttcc aatcccagcc catgatcggg cccgagaagc
     1201 cctccttgcg agctatcgcc tccaggtcct tctgctcctc gagcgccctg gcccgggccg
     1261 gcttccagac gcgcatcaag aggtcgtcga cggccgccgg cttcttggcc atacggtcga
     1321 cgagggcgta gtcggcgtag ctcgcgtagc ccatgaggcc ggccagctcg gctcgcagcc
     1381 ggaggatctt ctcggccacc ggcttcgtgt cgcgctcggc gcggctctcg ccgcgggcct
     1441 tgaacgcgcg ccagacctgc tcgcgcaggt cgcgccgcgt cgagtaggtc aggaaagggt
     1501 cgaccagcga tggcgacagg ttgatagcgt agccgcccag gcccttgtcg gccgcgacgg
     1561 acttggccgc gtcgagcacg aactcgggca ggccggcgcg gtcgtcgtcg gtcttgagct
     1621 cgaggcagaa ggccgcctcg t
//
LOCUS       DAOPHV010000625         1640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_107474_length_1640_cov_3.227129, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000625 DAOPHV010000000
VERSION     DAOPHV010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107474_length_1640_cov_3.227129"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="PLQ29_12765"
     CDS             <1..1233
                     /locus_tag="PLQ29_12765"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPG87562.1"
                     /translation="RLLYDVHCHAMSLARPAIGRFVDAFLRGGLQSFFSQVAAPGYIL
                     AALGRNMGESVRNLFAVVENDPDALLELIENDLSGLYLSDDEAHRSVILPGGPKLLGE
                     DWDGWLVCPLLMDFGARATSHDTYYCEPIRKSLAEGVREALAGVSGYRRERPNGRLVI
                     RPFLGLDPGARGASETEAALSTYFSGFSRALPAQLRAFKSAARWKGDPASPPRNTFAG
                     VKLYPPLGFDPWPEDQAALDATRLLYAFCERRGVPIVVHCDDQGYRTIALDLAMRYTD
                     PERWVSALKAYPDLVVDFAHFGERYLGRSRARGAWTESIIRLMERYPGVYADTAFNGC
                     EPAYWDRLDRFVASLPAATSEIVRSRLLFGTDFVISLTKTRSYLDYVTDFASSSLDDG
                     LKRAMMTVNPAAFLFGAS"
     gene            complement(1230..>1640)
                     /locus_tag="PLQ29_12770"
     CDS             complement(1230..>1640)
                     /locus_tag="PLQ29_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87563.1"
                     /translation="RAQQALGPRRPPNRLASVEQVDPSELLAEIAVRNLEPIAARNRA
                     ICSRNLGLLDGFFERRQDFARWTPPAAGSVAFPRLSDGSDAEELAERLVAESGVLLLP
                     GAYYDYDRSYFRLGFGRANLPEALDRFELWLERR"
BASE COUNT          241 a          599 c          521 g          279 t
ORIGIN      
        1 agactgctgt acgacgtaca ctgccacgcg atgtcgctcg cgcgccccgc gatcggccgc
       61 ttcgtcgacg ccttcctgcg cggcggcctc cagtcgttct tcagccaggt ggccgcgcca
      121 ggttacatcc tggccgccct tggccgtaac atgggcgagt cggtacgcaa cctgttcgcc
      181 gtcgtcgaga acgatcccga cgccctgctc gagctgatcg agaacgacct gtccggcctc
      241 tacctgtccg acgacgaggc gcatcggagc gtgatcctcc ccggcggccc gaagctactc
      301 ggcgaagact gggatggctg gctcgtctgc ccgctcctga tggatttcgg cgccagggcg
      361 acgtcgcacg atacgtatta ctgcgaacct atacggaaga gcctggccga gggcgtgcgc
      421 gaggccctcg ccggcgtctc cggctatcgg cgcgaacgcc ccaacggccg gctggtgatc
      481 cggcccttcc tgggcctcga ccctggtgcc cgcggagcct cggagacgga ggccgcgctg
      541 tctacctact tctcgggctt ctcgcgcgcg ctcccggccc aactgcgcgc cttcaagtca
      601 gcggcgcgct ggaagggcga tccggccagt cccccgagga acacgttcgc cggcgtcaag
      661 ctgtatcccc cgctcggctt cgacccgtgg cccgaggatc aggccgccct cgacgccacc
      721 aggctcctgt acgcgttctg cgagcggcgg ggcgtgccga tcgtcgtcca ctgcgacgat
      781 cagggctacc gcaccattgc gctcgacctg gcgatgcgct acaccgaccc cgagcgctgg
      841 gtgtcggctc tgaaggccta tcccgacctg gtcgtcgact tcgcccactt cggtgagcgc
      901 tatctcggcc gctccagggc ccggggcgcc tggaccgagt ccataatcag gctgatggaa
      961 cggtatcccg gcgtctacgc cgacacggcg ttcaacggtt gcgagcctgc gtactgggat
     1021 cggctggacc gcttcgtcgc ttccctgccc gccgccacgt ccgagatcgt ccgttcgcgg
     1081 ctactgttcg gcaccgactt cgtcatcagc ctgaccaaga ccaggtcgta cctcgattac
     1141 gtgaccgact tcgcgtcgtc ctcgctcgac gacgggctca agcgggcgat gatgaccgtg
     1201 aacccggcgg ccttcctgtt cggggccagc tagcgccgct ccagccagag ctcgaaccga
     1261 tcgagcgcct cgggcaggtt ggcccgcccg aagcccagcc tgaagtacga gcggtcgtag
     1321 tcgtagtacg ctcccggcag caatagcacc ccggattcgg ctaccagacg ctccgccagc
     1381 tcctcggcgt ccgatccgtc gctcagcctc gggaaggcca cggaacccgc cgctggcggc
     1441 gtccatcggg cgaaatcctg ccgccgctcg aagaagccgt cgagcaggcc caggttcctc
     1501 gagcagatcg cgcggttgcg agcggcgatg ggctccaggt tgcgcaccgc tatctccgcg
     1561 agcagctcgc tcgggtcgac ctgctcgacg ctagccagcc tattcggagg ccggcgaggc
     1621 ccgagtgctt gctgagcacg
//
LOCUS       DAOPHV010000626         1636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_107849_length_1636_cov_2.977862, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000626 DAOPHV010000000
VERSION     DAOPHV010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107849_length_1636_cov_2.977862"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..699)
                     /locus_tag="PLQ29_12775"
     CDS             complement(187..699)
                     /locus_tag="PLQ29_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HPG87564.1"
                     /translation="MYYNTAIAYLLWALGGFGALGFHRFYLRKIPTGLLWLCTGGLGM
                     VGSIYDFFTLSGQVREANIRAGYREALDMPRERVVIRERYVEPSQDRAHAKESIEKVI
                     LRTAKKNGGLVTPGEIAIESDYDSEAARSALEKLAAKGFCEMRVRPSGVIVYRFPEFA
                     AGDEGFEPGM"
     gene            809..>1636
                     /locus_tag="PLQ29_12780"
     CDS             809..>1636
                     /locus_tag="PLQ29_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPG87565.1"
                     /translation="MKDIKAILAERSVGSAITAKGWARTKRSTKGATFVELYDGSCFS
                     GLQCVFDSGAAAPGLEATLARVTTGASLAVSGVLVDSPAAGQPVELKATSVEVIGDCP
                     ADSYPLQKKAHSLEFLREIAHLRPRTNTIGAAARMRDEMAYAVHTFFHDRGFRYVHTP
                     LITASDCEGAGEMFQVTTLDLDAIAKRGEAVDYAKDFFGKKAFLSVSGQLNVETYCMA
                     LGKVYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYDLNDNMDLAEDFVKYLIRYALEN
                     "
BASE COUNT          309 a          529 c          497 g          301 t
ORIGIN      
        1 cttgcccatg cgctcgtggg aagggtccca gctacgcagg tcgagcttcg gctcgcgccc
       61 gttccacgag accaggttga gctcgaccgt ccagtccttg cccgacggcc cgagcacagc
      121 caggcggcga tcgatccgga attgaatcaa gtcgcggttc ctatccactc cgtacctccg
      181 cgctgtttac atacccggct cgaagccctc gtctccggcg gcgaactcgg ggaatcggta
      241 gacaatgacg cccgacgggc gcacgcgcat ctcgcagaag cccttggcgg ctagtttctc
      301 gagggcactc ctcgcggcct ccgagtcgta gtcgctctcg atggcaatct cgcccggagt
      361 gacgagcccg ccgttctttt tggcggtgcg caggatgacc ttctcgatcg attccttggc
      421 gtgcgcgcga tcctggcttg gctcgacata gcgctcgcgg atgacgacgc gctcgcgcgg
      481 catgtcgagg gcctcgcggt agccggcgcg gatgttggcc tcgcgcacct gccccgacag
      541 cgtgaagaag tcgtagatgg atccgaccat gccgaggccg ccggtgcaca gccagaggag
      601 gcccgtgggt atcttgcgta ggtagaacct atggaaaccc agggcgccga agcctccgag
      661 cgcccagagc aggtaggcaa tcgcggtgtt atagtacatt gatcactccg tacgatggat
      721 gcgcatatag taccacagaa tctcccgatg gaaaaatact gcgcgcgggg ctttgaccaa
      781 ggacggcgcc tagtggtatt actatgctat gaaggatatt aaagcgattc tggcggagcg
      841 atccgtaggc tccgcgatca cggccaaggg ttgggcccgc accaagcgct cgaccaaggg
      901 cgccacattc gtcgagctgt acgacggctc gtgcttctcc gggctccaat gcgtcttcga
      961 ctccggagcc gcggcgcccg ggctcgaggc gactcttgcc agggtgacga ccggcgcctc
     1021 gctggccgtg agcggcgtgc tcgtcgactc gcccgccgcg gggcagccgg tcgagctcaa
     1081 ggccacatcc gtcgaggtga tcggcgactg cccggccgat agctacccgc tccagaagaa
     1141 ggcccactcg ctcgagttcc tgcgcgagat agcccatctg cgcccccgga ccaatacgat
     1201 aggcgcggcg gcgcgcatgc gcgacgagat ggcctacgcc gtccatacct tcttccacga
     1261 ccgcggcttc cgttacgtgc atacgccctt gatcaccgcc agcgactgcg agggcgccgg
     1321 cgaaatgttc caggtgacca ccctcgacct ggacgcgata gccaagcgcg gcgaggccgt
     1381 cgactacgcc aaggacttct tcggcaagaa ggcctttctg tccgtctccg ggcagctgaa
     1441 cgtcgagacc tactgcatgg ccctcggcaa ggtgtatacc ttcggtccga ccttcagggc
     1501 agaaaattca aatacaccca ggcatcttgc cgagttctgg atgatcgaac cggaaatggc
     1561 attctacgac cttaatgaca atatggacct tgccgaggat ttcgtcaagt acctcataag
     1621 atacgctctt gaaaac
//
LOCUS       DAOPHV010000627         1635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_107920_length_1635_cov_4.021519, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000627 DAOPHV010000000
VERSION     DAOPHV010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107920_length_1635_cov_4.021519"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="PLQ29_12785"
     CDS             <1..926
                     /locus_tag="PLQ29_12785"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPG87566.1"
                     /translation="FSMRDLKAIGLLERLERTGSPADEIRIIREIGAYGTAVAERELL
                     GYLSSPRLDVRVEALLAMENLARLSAKALRAMIREVERHPYTTAYIAARVLGRRRCVE
                     ALPVLRSALGADDYMLRGAAVTALAALRDEESLPAIEALASDSDNPRLMINAASAIEA
                     FGRVSSVPALVAVLKRREPPPYAFDEIVLALSGILEGLGGFYRLYYDFELDRDGAVAA
                     LLEQLDGSAAIGAPPREEFSAALRAFVTRGERGEVVARAIAESSFLDEGSATVLAEAC
                     LDEELAAHRGFRFFLAACAVEARIASGQPKG"
     gene            931..>1635
                     /gene="proC"
                     /locus_tag="PLQ29_12790"
     CDS             931..>1635
                     /gene="proC"
                     /locus_tag="PLQ29_12790"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPG87567.1"
                     /translation="MGRTIGFIGAGKIVDIMLGALCPAFGDDELWVWNRTASRSEALA
                     ARYPVSVSPSVKDLAERSDWLFVAVKPYAVAKVLSEACLSVRDDALVISMAAGVTIDA
                     IKTHFAAPPAIVRIMPNLPLKAGCGMTAICPDPSVKQADIDALLALFSSVGRARLIEE
                     RLFDTVTALSGSGPAFAFLFIESMADGAVLNGMDRAQAYEFAAQTVMGAAKIILESDR
                     HPGELKDIVASPAGTTM"
BASE COUNT          235 a          591 c          551 g          258 t
ORIGIN      
        1 tattctcgat gcgcgacctc aaggccatcg gcctcctcga gcgtctggag cggaccgggt
       61 cgccggccga tgagatacgc atcatccggg agatcggcgc ctacggcacc gcggtggccg
      121 agcgggagtt gctcgggtac ctgtccagcc cgaggctcga cgtacgcgtc gaggccctgc
      181 tggccatgga gaacctggcc aggctttcgg ccaaggctct cagggccatg atccgcgagg
      241 tggagcggca cccctacacg accgcctaca tcgcggcgcg cgttctcggc aggcgccgct
      301 gcgtcgaggc gctaccggtc ctgcgctcgg cgctcggggc cgacgactac atgctccggg
      361 gcgccgcggt gacggcgctc gccgcgctcc gcgacgagga gagcctgccg gcgatcgagg
      421 ccctcgccag cgacagcgac aacccgcggc tcatgatcaa cgccgcctcg gccatcgagg
      481 cgttcggccg ggtctcgtcg gtgccggctc tcgtcgccgt gctcaagcgc agggagccgc
      541 cgccctacgc gttcgacgag atcgtgctcg ccctgtccgg catactcgag ggccttggcg
      601 gtttctaccg gctctattac gacttcgagc tcgaccgcga cggcgccgta gccgccctgc
      661 tcgagcagct cgacggctcg gccgcgatag gcgccccccc gagggaggag ttttcggcgg
      721 cgctccgggc cttcgtcacg cgcggggagc gcggcgaggt cgtggccagg gccattgccg
      781 aatccagctt cctcgacgag ggctccgcca cggtgctggc cgaagcctgc ctcgacgagg
      841 agctggcggc tcaccgcggc ttcaggttct tcctggccgc ctgcgcggtc gaggcgcgaa
      901 tcgcatcggg acagccgaaa ggatgacact atgggtagga cgataggatt cataggagcc
      961 ggcaagatcg tcgacatcat gctcggcgcg ctctgcccgg ccttcggcga cgacgagcta
     1021 tgggtctgga accggaccgc ctcgcggtcg gaggcgctgg ccgcccgcta cccggtcagc
     1081 gtatcgccaa gcgtcaagga cctggccgag cgttccgact ggctcttcgt cgccgtcaag
     1141 ccctacgcgg tggccaaggt cttgtccgag gcctgcctga gcgtgcgaga cgacgccctg
     1201 gtcatctcga tggccgccgg cgtcaccatc gacgcgatca agacgcactt cgcggcgccc
     1261 ccggccatcg tgcgcatcat gccgaacctg ccgctcaagg ccggctgcgg catgaccgcc
     1321 atctgcccag acccttccgt caagcaggcg gacatcgacg ccctgctggc gttgttctcg
     1381 tcggtcggcc gggcccgcct catcgaggag cgcctcttcg acacggtcac cgccctgtcc
     1441 ggctcggggc cggccttcgc cttcctgttc atcgagtcga tggccgatgg ggccgtgctc
     1501 aacggcatgg accgggccca ggcctacgaa ttcgcggccc agaccgtgat gggggccgcc
     1561 aagatcatcc tcgagtccga ccgccacccc ggcgagctca aggacatcgt cgcctcgccg
     1621 gccggcacca cgatg
//
LOCUS       DAOPHV010000628         1633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_108121_length_1633_cov_3.250317, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000628 DAOPHV010000000
VERSION     DAOPHV010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108121_length_1633_cov_3.250317"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PLQ29_12795"
     CDS             complement(<1..433)
                     /locus_tag="PLQ29_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87568.1"
                     /translation="MKRSTALTVAIFAAFSAFGQGSGPATGETVGVAFVGFDSGDISE
                     SGRLILSDLVRREISIAPGFALVDRGNVELLLEEQELRLSEAFDAADPVRVGELTGAG
                     SYIVGRIGLLGTLYIISLRMIDVETGQVTRTATTALAPQQMW"
     gene            complement(587..1555)
                     /locus_tag="PLQ29_12800"
     CDS             complement(587..1555)
                     /locus_tag="PLQ29_12800"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPG87569.1"
                     /translation="MGYYFDARALARWGIGRESLPQGSVILYDEKSFWDRYWYALVIG
                     AAVFALESLLILALVRSSSQRAKAMRALAVERSRLELTVQARTADLSAANDDLVREIG
                     DRERAESKAVAMAAEKAMLLRELQHRVKNSLTIIVSLIGLESSKSGSAELRESLESLE
                     SRVSALAALYDILYDTGGIERLPLAAYLDRVVDAAAESLGADEKGISLTRDIAPIAMD
                     MKRAVSLGLIVNELVTDCLKYAFPGGARGTVRVSLRAEAGRYLLVVEDDGVGLPEGFD
                     PDSVDGFGFKLVGMLARQLHGEFSWSSRDGSRFSLSIPAEDQASSG"
BASE COUNT          274 a          544 c          556 g          259 t
ORIGIN      
        1 cccacatttg ttggggcgca agggccgtcg ttgcggtgcg cgtcacctgg ccggtttcga
       61 cgtctatcat ccgcagagag atgatgtaca gggtgcccag gaggccgata cggccgacga
      121 tatacgagcc ggcgccggtc agttcgccga cacgcaccgg gtcggcggcg tcgaaggcct
      181 cggacaggcg cagctcctgc tcctcgagca ggagctcgac gttgccgcgg tcgacgagcg
      241 cgaagccggg ggcgatggat atctcgcgac ggaccaggtc ggacaggatc agcctgccgg
      301 attcgctgat atcgcccgag tcgaagccga cgaaggccac cccgacggtc tcgcctgtgg
      361 ccggacccga gccctgcccg aaggcggaga aggccgcgaa gatggcgacg gttaatgcgg
      421 tggatcgttt catacgcttt cgaggcgacc gctcggcggc tcgacgccgg ggcgcgagcg
      481 aggcctcacc tccccgatgg ccggctagcg ccgttccgtg cagtacggcg gcgccgcgct
      541 tctaagtata tcccgaaaac cggtcgagcg gccatgcgtt tcgtcgtcat cccgaggagg
      601 cttggtcttc ggccggtatg gagagcgaga accgggagcc gtcgcggctg gaccaggaga
      661 actcgccatg gagctgcctg gccagcatcc cgacgagctt gaagccgaag ccgtcgaccg
      721 agtcggggtc gaagccctcg ggcaacccga cgccgtcgtc ctcgacgacg agcaggtagc
      781 ggccggcctc ggcccgtagg ctgacccgta ccgttcctcg agccccgccg gggaaggcgt
      841 acttgaggca gtcggtcacg agctcattga cgatgagccc gagcgacacg gcccgcttca
      901 tgtccatcgc gatgggcgcg atgtccctcg tcaggctgat acccttctcg tcggccccga
      961 gactctcggc cgcggcgtcg acgacacggt cgaggtaggc cgccaggggc aggcgctcga
     1021 tgccgccggt gtcgtagaga atgtcgtaga gggccgcgag cgccgatacc ctcgactcca
     1081 gcgactccaa cgactcgcgc agctcggccg agccggactt gctcgactcg agccctatga
     1141 ggctgacaat aatggtaagg ctgttcttga cgcgatgctg cagctcgcgg agcagcatcg
     1201 ccttctccgc cgccatggcc acggccttgg actcggcgcg ctcgcggtca ccgatctcgc
     1261 ggaccaggtc gtcgttcgcc gcggacaggt cggcggtgcg ggcctggacg gtcagctcga
     1321 gcctggagcg ctcgacggcg agggcccgca tcgccttggc ccgctgcgac gacgagcgta
     1381 cgagggccag gatcaagagc gactccaagg cgaagacggc ggctcctatg accagcgcgt
     1441 accagtacct atcccagaag ctcttctcgt cgtacaggat gacgctgccc tggggcagcg
     1501 actcgcggcc gatgccccag cgggccaagg cgcgggcgtc gaagtagtag cccatcgtcg
     1561 cctcggcgta gccggtctgt aaccgccaat ttcaccccgt cttgcgttcc gcttaaaagc
     1621 tgataccgtt gcc
//
LOCUS       DAOPHV010000629         1630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_108393_length_1630_cov_4.294603, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000629 DAOPHV010000000
VERSION     DAOPHV010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108393_length_1630_cov_4.294603"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1187)
                     /locus_tag="PLQ29_12805"
     CDS             complement(12..1187)
                     /locus_tag="PLQ29_12805"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPG87570.1"
                     /translation="MARVLAIDDDPSYLESMTDYLRLKGHEAAGVTSPPEPGAITGTY
                     DIVLLDIALDGESGFDWLERYAAAGVPTAMVSGLADARTAVRAVKAGAVDVLEKPVDP
                     ERLEVVIAMAEEKAALARSDRAGRDAWLAEHLYVGGSEAMRSLVEVAERGAATSLCVL
                     LHGPSGSGKDPLARWIHFRSRRSAGPFVAVNCASIPADLAESELFGHRRGSFTGADRD
                     RAGCFAEAAGGTLFLDEVGELPPALQPKLLRAIESGEFRPLGADSPRRSDARIVAATN
                     RDLKAEAAKGAFREDLLYRLAQIPLAVPPISARVDDITALARFFAGRLGGRGFSDDAL
                     EYLGRRDYPGNVRELRNLVERALALAPEAAEVDASAVRAVDETGSHRQNVVSPVRSQ"
     gene            complement(1180..>1630)
                     /locus_tag="PLQ29_12810"
     CDS             complement(1180..>1630)
                     /locus_tag="PLQ29_12810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG87571.1"
                     /translation="EYYDGEAVATLRAKGATISDDSLTIERPDGETLARFDRGYLVEA
                     FINLLDNSIEACAERSIPFGARLRLGTEGQVYVIEYSDSVVGLPAELVERVGSARFST
                     KPGGSGLGVWLIGRIAALHGGETRIRLSDSGGLVFTLVFPNGGEDDG"
BASE COUNT          251 a          560 c          565 g          254 t
ORIGIN      
        1 tcaggcaccg actactgtga tctcactggc gagactacgt tctggcgatg gctgcccgtt
       61 tcgtcgacgg cgcgcacggc cgaggcgtcc acctcggccg cctcgggggc tagggcgagg
      121 gctcgctcga cgaggttgcg cagctcgcgg acgttgccgg gatagtcgcg ccgtccgagg
      181 tactcgaggg cgtcgtccga gaagccgcga ccgccgagcc tgccggcgaa gaaacgcgcc
      241 aaggccgtta tatcgtcgac cctggccgat atgggcggaa ccgccagcgg tatctgcgcg
      301 agccggtaga ggaggtcttc cctgaaggcg cccttcgccg cctcggcctt gaggtcgcgg
      361 ttggtggccg ccacgatgcg agcgtcggag cggcgtggcg agtcggcgcc gagcggccgg
      421 aactcgcccg actcgatagc ccggagcagc ttgggctgca aggccggcgg cagctccccg
      481 acctcgtcca ggaacagcgt gccgccggcc gcctcggcga agcagccggc ccgatcgcgg
      541 tcggccccgg tgaaggaacc cctgcggtgg ccgaacaact cgctctcggc caggtctgcg
      601 gggatggacg cgcagttgac cgcgacgaag gggccggccg agcgtctgga acgaaagtgt
      661 atccacctgg cgagcgggtc cttgcccgag ccgctcggcc cgtggagcag cacgcagagc
      721 gacgtcgccg ctccccgctc cgcgacctcg acgagagagc gcatcgcctc ggagccgccc
      781 acgtagaggt gctcggctag ccaggcgtcg cgaccggccc ggtcggatcg cgccagggcg
      841 gccttctcct cggccatcgc gatgaccacc tcgagtcgct ccgggtccac gggcttctcg
      901 aggacgtcga cggccccggc cttgacggcc ctcacggccg tcctggcgtc tgccaggccc
      961 gagaccatcg cggtaggcac gccggccgcc gcgtagcgct caagccagtc aaagccgctc
     1021 tcgccgtcga gggctatgtc gagcaacacg atatcgtagg ttccggtgat cgcgcccggc
     1081 tctggaggac tcgtcacgcc ggccgcctca tggcccttga gcctcaggta gtcggtcatc
     1141 gactcgaggt acgacgggtc gtcgtcaatg gccagcactc tagccatcgt cttctcctcc
     1201 gttcgggaat acgagcgtga acacgagccc gcccgagtcg gatagcctta tccgcgtctc
     1261 gccaccatgc agagcggcta tgcggccgat gagccagacg ccgaggcccg agccgccggg
     1321 cttggttgag aagcgggccg agcctacgcg ttcgactagc tcggccggca gcccgactac
     1381 cgagtctgag tactcgatga catagacctg tccttcggtg ccgagccgga ggcgcgcccc
     1441 gaaggggatg gagcgttcgg cgcaggcctc gatgctgtta tcgagcaggt tgatgaaggc
     1501 ctcgacgagg tagcctcgat cgaacctggc gagggtctcg ccatcggggc gctcgatggt
     1561 caggctatcg tcggatatgg tagcgccttt cgcccgtaac gtcgcgaccg cctcgccgtc
     1621 gtagtactcg
//
LOCUS       DAOPHV010000630         1628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_108624_length_1628_cov_3.076287, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000630 DAOPHV010000000
VERSION     DAOPHV010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108624_length_1628_cov_3.076287"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      <1..96
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            245..1144
                     /locus_tag="PLQ29_12815"
     CDS             245..1144
                     /locus_tag="PLQ29_12815"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HPG87572.1"
                     /translation="MKRVIMSLCALAIAVAAAAADPVSFVDERGATISMPGKARRVVS
                     LSPELTESLFAAGAGDAVVGVTTYCNYPAEASGRTKIGGFSAKTISVETIVALKPDLV
                     VGGLSAHGQLAAQFERAGLRFAALPTTDFDDIYATIELLGRVAGDETTALALVADMKT
                     RLAAVSAKIAAVPVSERPLVFWETWDEPLMSAGPGTFTGQVIEAAGGRNCFADATSDW
                     PVVSFEALLARDPDYIMAADSHGEALTPERLAKRSGWTSLRAVRNGKLVFLDGDIVAR
                     AGPRFVEAVELMARALYPDLYDR"
     gene            1154..>1628
                     /locus_tag="PLQ29_12820"
     CDS             1154..>1628
                     /locus_tag="PLQ29_12820"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HPG87573.1"
                     /translation="MSKDRNQAALAYLGLVTLLVASFAAAIALGAVRLSPAEAARALL
                     SSRRAWPGAAGSSGEASSPEAIIVGIRFARACLAAAVGASLAASGAVFQGLFRNPLAD
                     PYVIGSSGGAALGAVCAIAFGSGAGALGFSGIGLAAFAGGLGATLIVYLVAGATRL"
BASE COUNT          227 a          603 c          554 g          244 t
ORIGIN      
        1 aatccggtga gaatccggag ctagcccgta actgtaggcc gcgcccgctc cgggcgccgg
       61 cgagccagga tacccgcgcc ggcgtgatct tcgtggaacg cttcgtggta caggcgtaaa
      121 gcgatagtcc ggcggccagg cggcgccggc ccgtcgatgg caccccgatc aggaaccgtt
      181 cctccgtcac gcgcgcacgg catccgcgac atagcggcgc ccgcgcccga tcaaggagga
      241 agctatgaaa cgcgtcatca tgtcgctctg cgcgctcgcg atcgccgtcg cggcggccgc
      301 ggccgacccc gtatcgttcg tcgacgagcg aggagcgacg atcagcatgc ccggcaaggc
      361 gaggcgcgtc gtgtcgctgt cgcccgagct gaccgagtcg ctgttcgcgg ccggagccgg
      421 agacgcggtc gtcggcgtga ccacctactg caactacccg gccgaggcgt cgggcaggac
      481 caagataggc ggcttctccg ccaagacgat cagcgtcgag accatcgtcg cgctcaagcc
      541 ggacctcgtg gtcggcggcc tctcagccca cggccagctc gccgcccagt tcgagcgcgc
      601 cggcctgcgc ttcgccgcgc taccgacgac cgacttcgac gatatatacg cgaccataga
      661 gctgctcggg cgcgtagccg gggacgagac gaccgcgctc gcgctcgtag ccgatatgaa
      721 gaccaggctc gccgccgtca gcgcgaagat cgccgccgta cccgtctcgg agcggccgct
      781 cgtgttctgg gaaacctggg acgagccctt gatgagcgcg ggtcctggca ccttcacagg
      841 ccaggtaatc gaggcggccg gcggccgcaa ctgcttcgcg gacgcgacct ccgactggcc
      901 ggtcgtcagc ttcgaggcgc tgctcgcgcg cgaccccgac tacatcatgg ccgccgacag
      961 ccacggcgag gccctgacgc ccgagcgcct cgccaagcgg tccggctgga cgtcgctacg
     1021 ggccgtcagg aacggcaagc tcgtgttcct cgacggcgac atagtcgcgc gcgccggccc
     1081 gcgcttcgtc gaggccgtcg agctgatggc gcgcgccctg tacccggacc tgtacgacag
     1141 gtagcggcgg cgcatgtcga aggacaggaa ccaggccgcc ttggcctacc taggactcgt
     1201 cacgctgctc gtggcgtcgt tcgcggcggc catagccctc ggcgcggtca ggctatcgcc
     1261 ggccgaggcc gcccgagccc tgctatcgtc gcgcagggcc tggcctgggg ccgccggctc
     1321 gtccggcgag gcttcgtcgc cggaagctat catcgtcggc atcaggttcg cccgcgcctg
     1381 cctcgccgcg gcggtcggcg cgtccctggc cgcctcgggc gccgtgttcc agggcttatt
     1441 ccgcaatccg ctcgccgacc cctacgtcat cggtagctcc ggcggcgccg ccctcggcgc
     1501 ggtctgcgcg atagccttcg gctcgggggc tggagcgctc ggcttctcgg gcatcggact
     1561 cgcggccttc gctggcgggc tcggcgccac cctcatcgtc tacctcgtcg ccggcgctac
     1621 ccgcctcc
//
LOCUS       DAOPHV010000631         1626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_108813_length_1626_cov_2.916614, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000631 DAOPHV010000000
VERSION     DAOPHV010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108813_length_1626_cov_2.916614"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PLQ29_12825"
     CDS             <1..278
                     /locus_tag="PLQ29_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPG87574.1"
                     /translation="FEVDKLAQADPAYANMICRCEKVSEAEIVQAIRAGHTTLDGIKY
                     FTRAQMGRCQGGFCTYKIIKIIMRETGLSWDEITKNGDGSRILKDAL"
     gene            275..1531
                     /locus_tag="PLQ29_12830"
     CDS             275..1531
                     /locus_tag="PLQ29_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG87575.1"
                     /translation="MKDLVFDSVVIGGGAAGMAAALELDRAGFSCAIVEREEALGGIL
                     TQCIHNGFGLIEFNEELSGPEFAERIEERLAGTRITSFTGATVMELDASGDIKTLYCY
                     TGAHGVMRLSARAVVLAMGCRERNRGNIRIPGDRPAGVYTAGLAQRLVNIEGFVPGKD
                     VVIIGSGDIGLIMARRMSWIGCKVHAVVEILPYPSGLTRNIVQCLHDFGIPLYLSHLT
                     TNIVGRERVEGVEITPIENGATRFDKAFTIPCDTVLLSVGLVPENELSKMAGVELNPM
                     TNGPVADSMLMTSVPGVFACGNVLHVHDLVDYVVEEARRAGAHAARWLKGERPKAEAR
                     VKAGPNVRYCAPSRVDLHAANKLYMRTLIVKNDATLEVKVNNRVVKSVKKNHIQPSEM
                     IAVELGPKDLEFAVGAPDPVVELSIS"
BASE COUNT          313 a          541 c          516 g          256 t
ORIGIN      
        1 ccttcgaggt cgacaagctg gcccaggccg acccggctta cgccaacatg atctgtcgct
       61 gcgagaaggt gtcggaggcg gagatcgtcc aggccatcag ggctgggcat accacgctcg
      121 acggcatcaa gtacttcacg cgcgcgcaga tgggccgctg ccagggcggc ttctgcacct
      181 ataagatcat caagatcatc atgcgcgaga ccggcctctc gtgggacgaa ataaccaaga
      241 acggcgacgg cagccgcata ctcaaggacg ctctatgaaa gacctcgttt tcgactcggt
      301 agtgataggc ggcggggcgg ccggcatggc cgccgccctc gagctggacc gcgccggttt
      361 ctcctgcgcc atcgtcgagc gcgaggaagc gctcggcggc atcctgacgc agtgcatcca
      421 caacggcttc ggcctcatcg agttcaacga ggagttgtcc ggccccgagt tcgccgagcg
      481 gatcgaggaa cgcctggccg ggacgcgcat cacctcgttc accggcgcca ctgtgatgga
      541 gctggatgcc tccggtgata tcaagacgct gtactgctac accggcgccc atggcgtgat
      601 gcggctctcc gcccgcgccg tcgtgctggc tatgggctgc cgcgagcgca accgaggcaa
      661 catccgcata cccggtgacc gccccgcagg cgtttatacc gccggcctgg cccagcgcct
      721 ggtcaacatc gagggcttcg tgcccggcaa ggacgtcgtc atcatcggct ccggcgacat
      781 cggcctgatc atggccaggc gcatgagctg gataggctgt aaggtgcacg ccgtcgtcga
      841 gatcctgccg tacccgtccg ggctgacgcg caacatcgtc cagtgcctgc acgacttcgg
      901 catcccactg tacctgtcgc accttaccac caacatcgtc gggcgcgagc gggtcgaggg
      961 cgtggagatc accccgatcg agaacggcgc cacgcgcttc gacaaggcct tcacgatacc
     1021 ctgcgacacg gtcctcttgt ccgtcggcct cgtgcccgaa aacgagctgt ccaagatggc
     1081 cggcgtcgag ctgaatccga tgacgaacgg ccccgtggcc gactccatgc tgatgaccag
     1141 cgtgccgggc gtgttcgcct gcggcaacgt gctgcacgta cacgacctgg tcgactacgt
     1201 ggtcgaggag gctaggcggg ccggcgccca cgccgcgcgc tggctcaagg gcgagcgacc
     1261 caaagccgag gccagggtca aggcggggcc gaacgtgcgc tactgcgcgc cgtcccgcgt
     1321 cgacctgcac gcggccaaca agctgtacat gaggaccctg atcgtcaaga acgacgcgac
     1381 cctcgaggtc aaggtgaaca accgcgtcgt caagagcgtc aagaagaacc atatccagcc
     1441 gtcggagatg atcgcggtcg agctggggcc gaaggacctc gaattcgcgg tcggcgcgcc
     1501 cgaccctgtc gtcgagctgt ccatcagctg acgctgaagc ggaggagacc accatgagcg
     1561 aagaactgat atgcatcacc tgcccgctcg gctcgacaaa cgacaacagt acttgaccgg
     1621 caggat
//
LOCUS       DAOPHV010000632         1616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_109821_length_1616_cov_3.796284, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000632 DAOPHV010000000
VERSION     DAOPHV010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109821_length_1616_cov_3.796284"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..>1616
                     /locus_tag="PLQ29_12835"
     CDS             27..>1616
                     /locus_tag="PLQ29_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPG87576.1"
                     /translation="MPEIAIRRRLYRSGESEYFINGQPAKLKELRELFWDTGIGKSAY
                     SVMEQGRIDQILSSKPEERRYLFEEAAGITKHKARSREAELKLERTEENMRQVEGILG
                     EVKRSHDTLKVQAEKTLSYRSLREEVFNAELDLHLLRLRGFVNDKERRDGEIAQRTAE
                     RDALKAQIDGINLSLEENLDIVNSMEAKLVDVQKTVYGLAVERVGKEKERKLLSERVA
                     EAKAKIDQARLKLKALEEKLETLREDEDEKNDILRGHKGRIAEIEKNILGFEGSIKNA
                     EERIKANDQAIARSEADIVAFDEELVSAQAELDAITEDIVSELDARLKDIGYSAAERK
                     AAEEAIDALIAGIIARLSGKAAILDDLSRLKDYGAAEARALIENARTAMAESADRARE
                     LKERYEAYRLTTPSFIDEFLSPEGIITKKRSVDGRMAAIKDGVTERRERIATAREENR
                     ELASRIDEYRKTLEELRGNRIRMQTQAQSAEEQLAMIRREIASQEGYYRELDAEAAME
                     GRRLSEAEEDLSSIDEEIADIE"
BASE COUNT          360 a          489 c          549 g          218 t
ORIGIN      
        1 acgagacgtc cgtccttccc atggacgtgc ccgagatagc aatacggcgc cgcctgtacc
       61 gttccgggga gagcgagtac ttcatcaacg gccagccggc caagctcaag gaactgcgcg
      121 agctgttctg ggacacgggc ataggcaaga gcgcctactc ggtgatggag cagggccgca
      181 tcgaccagat cctgtcgagc aagcccgagg agcgccgcta tctgttcgag gaggcggccg
      241 gcatcacgaa gcacaaggcg cgctcccgcg aggccgagct caagctcgag cgcaccgagg
      301 agaacatgcg ccaggtcgag ggcatactcg gcgaggtcaa gcgcagccac gacaccctca
      361 aggtccaggc cgagaagacg ctgtcgtacc ggtccctgcg cgaggaggtg ttcaacgccg
      421 agctcgacct gcacctgctc aggcttcgag gcttcgtcaa cgataaagag cggcgcgacg
      481 gcgagatagc ccagcgcacg gccgagcgcg acgcgctcaa ggcccagatc gacggcatca
      541 acctgtcgct cgaggagaac ctcgacatcg tcaactcgat ggaagctaag ctcgtcgatg
      601 tgcagaagac ggtctacggc ctggccgtgg agcgggtggg taaggagaag gagcgtaagc
      661 tcctgtccga gcgcgtcgct gaggccaagg ccaagatcga ccaggccagg ctcaagctca
      721 aggcgctcga ggagaagctc gagacgctac gcgaggacga ggacgagaag aacgacatcc
      781 ttcgcgggca caagggtcgc atcgccgaga tcgagaagaa catcctcggc ttcgagggct
      841 cgatcaagaa cgccgaggag aggatcaagg ccaacgacca ggctattgcc cgttcggagg
      901 ccgacatcgt cgctttcgac gaagagctgg taagcgctca agccgagctg gacgccataa
      961 ccgaagacat cgtctccgag ctcgatgcga ggctcaagga tatcggctat tcggcggccg
     1021 agcgtaaggc ggccgaggag gccatcgacg cgctgatagc tggaatcatc gcgcgcctgt
     1081 ccggtaaggc ggccatactc gacgacctgt ccaggctcaa ggactatggc gcggccgagg
     1141 ctcgggcgct gatcgagaac gccagaaccg cgatggccga gtcggcagac cgggcccgcg
     1201 agctcaagga acgctacgag gcgtaccgcc tgacgacgcc gtcgttcatc gacgagttcc
     1261 tgtcccccga gggcatcatc accaagaagc gttccgtcga cgggcgcatg gcggcgatca
     1321 aggacggcgt gacggagcgg cgcgagcgca ttgccacggc ccgcgaggag aaccgcgagc
     1381 tggcttcccg tatcgacgag taccgtaaga ctctcgagga gttgcgcggc aatcgtatca
     1441 ggatgcagac ccaggcccag tcggccgaag agcagctcgc catgatcagg cgggagatag
     1501 ccagccaaga gggctactat cgcgagctcg acgccgaggc cgcgatggag ggccgcaggc
     1561 tgtcggaggc ggaggaggat ctctcgtcga tcgacgagga gatcgccgat atcgag
//
LOCUS       DAOPHV010000633         1613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_110180_length_1613_cov_2.874840, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000633 DAOPHV010000000
VERSION     DAOPHV010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110180_length_1613_cov_2.874840"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..>1613
                     /locus_tag="PLQ29_12840"
     CDS             118..>1613
                     /locus_tag="PLQ29_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG87577.1"
                     /translation="MKIRTAFSLFVAVVLIVGVAVSLLMGILPAREYIQKNFYASVGN
                     IVDASKSGIQTEIARGWEVSLALARNPFLADWLLGDERDTAAGRKVLSMAVEYASRTG
                     FSSSFIANRSTGSFYSGDKLMQVLSPTRADDSWFFDVLGSDKELTLNLDYNEQLGSTS
                     LWFNTQVLKGRAVIGVAGIALSIDKVVNDFKAAVPSANSGLHLVDSSGMVVVSSDDEA
                     IGKELRSLISGTAKSIPGLADVKTFDDPVLGPTLLSEEQILESGYSIAFVGPVSDFVP
                     SFISLSGGSILFTAVFAALVVTGAVLFISRRFAAPILEMNRLALGLAAGDLSVSADAS
                     ISRRKDEIGTLAQSMSATVGRLASVVSEVQATANKVASESDEMSSAARQMAIGIAGIA
                     DSSQQLSQGASEQAASAEQVSASVEQMGANIKQNADNSFQTEKIATKAAGDARQGAEA
                     VAQTVLAMRQIAEKIGIIEEIARSTNMLSLNASIEAARAGEHGKGFAVV"
BASE COUNT          322 a          492 c          524 g          275 t
ORIGIN      
        1 acggtcaaga aaccgtcgac cgcgatcacg ctcaaggcgc agcccaagcg cgactcccgt
       61 gacgacgacg atttcattga gtactagcac gagggataca ggaagcgagg acaggatttg
      121 aagatacgga ccgccttttc actattcgta gccgtcgtcc tcatcgttgg agtggcggta
      181 tccctgctga tgggcatcct gccggcgcgc gaatatatac agaagaattt ctacgccagc
      241 gtgggcaata tcgtagacgc ctcgaaatcg ggcatacaga ccgagatagc ccgcggctgg
      301 gaggtgtctc tggcgttggc caggaatccg ttcctcgccg actggctgct cggcgacgag
      361 cgggacacgg ccgccggccg caaggtgcta tcgatggccg tcgagtacgc ctcgaggacc
      421 ggcttctctt cgagcttcat agcgaaccgt agcaccggat cgttctactc tggagataag
      481 ctcatgcagg tcctgtcgcc cacgagggcg gacgactcct ggttcttcga cgtgttgggc
      541 tccgataagg aactgacctt gaacctcgac tataacgagc agctcggctc gacgtcgcta
      601 tggttcaaca cccaggtgct aaagggccgg gcggtcatag gcgtcgccgg catcgcgctg
      661 tccatcgaca aggtcgtcaa cgacttcaag gcggccgtgc cgagcgcgaa cagcgggctc
      721 cacctggtgg actcgtccgg tatggtggtc gtctcgtccg acgacgaggc gatcggcaaa
      781 gagctgcgct cgctaataag cgggacggcg aagagcatcc ctggcctggc cgatgtcaag
      841 accttcgacg acccggttct cggtccgacc ctgctgtccg aggagcagat actggaatcc
      901 ggctactcca tcgccttcgt cggtcccgtg tcggacttcg tgccgtcgtt catcagcctg
      961 agcggcggtt cgatactatt cacggccgtc ttcgcggccc tcgtcgtcac gggcgccgtg
     1021 ctgttcatat cgaggcgttt cgcggcgccg attctggaga tgaaccggct ggcgctcggg
     1081 ctggccgccg gcgacctgag cgtcagcgcc gacgcatcga tatcgcggcg caaggacgag
     1141 atagggacgc tggctcagtc catgagcgcg acggtgggac ggctggcgag cgtcgtctcg
     1201 gaggtacagg ccacggccaa caaggtcgcg agcgaatccg acgagatgtc atcggccgcc
     1261 aggcagatgg ctataggtat agccggcata gccgattcca gccagcagct gtcccagggc
     1321 gcgtccgaac aggccgccag cgcggaacaa gtatcggcaa gcgtcgaaca gatgggcgcc
     1381 aacatcaagc agaacgccga caactcgttc cagaccgaga agatagccac caaggccgcc
     1441 ggcgacgcca ggcagggcgc cgaggcggtc gcccagaccg tgctggcgat gcgtcagata
     1501 gccgagaaga tcggcatcat cgaggagatc gcgcgatcga cgaacatgct gtcactgaac
     1561 gcgagcatcg aggcggcgcg ggccggcgag cacggcaagg gcttcgccgt cgt
//
LOCUS       DAOPHV010000634         1609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_110577_length_1609_cov_4.483269, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000634 DAOPHV010000000
VERSION     DAOPHV010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110577_length_1609_cov_4.483269"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLQ29_12845"
     CDS             complement(<1..448)
                     /locus_tag="PLQ29_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87578.1"
                     /translation="MAEARRLASLAAAMLFAASAMVGADPAPSGWAPGAADSARRAPA
                     GTPQEAAAALLYGVPDGLALASIVGKPTLLGTVNYLFKDGDERRLAGYVDVVAVYDLP
                     LEAMAAALVDFEAAPGYVPHILAATVRRRGPDGVEIYYKSGFRVLGI"
     gene            complement(451..1347)
                     /locus_tag="PLQ29_12850"
     CDS             complement(451..1347)
                     /locus_tag="PLQ29_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPG87579.1"
                     /translation="MAKYGLGKGLEALIPESAPESPKSDAGRTDGGVASVPLSRVSPS
                     ADQPRKRFDDQSIAELADSIRRHGVIQPLILEADGSGGYRIVAGERRWRAATLAGLAE
                     VPAIVKEYGLEKRLEIALVENVQREDLNPIDEAEAYRQLMEATGLTQDQVAERVGKSR
                     PAVANALRLLGLPADAMEALRSGAISAGHARAILSAVDPAHRRTILRRAVDDGISVRE
                     AEALAAACNSGGKPADKKPAKQAEPRPLDPNLAAIEQDLIGRLGTKVAIRGDASRGSI
                     TIDYFSMDDLDRVFGIIAGGGS"
     gene            complement(1340..>1609)
                     /locus_tag="PLQ29_12855"
     CDS             complement(1340..>1609)
                     /locus_tag="PLQ29_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPG87580.1"
                     /translation="IQLVQKGMNPGLKITGILFTMYDSRTRLAQDVVQQVSSYFGERV
                     FSTIIPRNVRLSEAPSHGVPICLYDPACIGAKSYEKLAAEVLARG"
BASE COUNT          226 a          553 c          574 g          256 t
ORIGIN      
        1 cgatgccgag gacgcggaag cccgacttgt agtatatctc gacgccgtcg gggcctcgtc
       61 gccggacggt cgccgcgagt atatgcggaa cgtagccggg cgccgcctcg aagtccacca
      121 gggcggcggc catcgcctcg aggggcagat cgtagacggc cacgacatcg acatagccgg
      181 cgagccgcct ctcgtcgccg tccttgaaga ggtagttcac cgtgccgagg agggtcggct
      241 tgccgactat cgacgcgagc gcgagcccgt cggggacgcc gtagagcagg gccgcggccg
      301 cctcctgcgg ggtgcctgcc ggagcgcgcc tggccgaatc cgcggccccc ggcgcccatc
      361 cgctcggcgc ggggtcggcg ccgaccatcg cgctggccgc gaacagcatc gcggcggcca
      421 gcgaggcgag gcgccgcgcc tcggccatcg ctagctcccg cccccggcta tgatgccgaa
      481 gacccggtcc aggtcgtcca tcgagaaata atctatcgtg atcgagccgc gcgaggcgtc
      541 accccggatc gccaccttgg taccgagtcg gcctatcagg tcctgctcga tggccgccag
      601 gttcgggtcg agcggccgcg gctcggcctg cttggccggc ttcttgtcgg ccggcttgcc
      661 gcccgagttg caggcggccg cgagggcctc ggcctcgcgc acggagatgc cgtcgtccac
      721 ggcccggcgc agtatggtcc gcctatgggc ggggtctacg gccgatagga tggcgcgggc
      781 gtggccggcg ctgatcgcgc cggagcgcag ggcctccatc gcgtcggccg gcaggccgag
      841 cagcctgagc gcgttggcca ccgcggggcg gctcttgcca acccgctcgg ccacctggtc
      901 ctgggtcaag ccggtggcct ccatcagctg acggtaggcc tcggcctcgt ctatcgggtt
      961 gaggtcttcg cgctgcacgt tctcgacgag ggctatctcg agccgcttct cgaggccgta
     1021 ttccttgacg atggccggca cctcggccag cccggccagc gtggccgccc gccagcggcg
     1081 ctcgccggcg acgatgcgat agccgccgga gccgtcggct tccaggatga gcggctggat
     1141 gacgccgtgg cgccgtatcg agtcggccaa ctcggctatc gactggtcgt cgaagcgttt
     1201 acgcggctga tcggcgctcg gggaaaccct ggacagcggc acggatgcca cgccgccgtc
     1261 tgtgcggccc gcgtcggact tgggagactc gggggcgctc tccggaatca gggcctcgag
     1321 ccccttaccc agtccgtact tagccacggg cgagaacctc ggcggctagc ttctcgtagc
     1381 tcttggcgcc tatgcaggcc gggtcgtaca ggcagatcgg cacgccgtgg gagggcgcct
     1441 ccgacagcct gacgttacgc gggatgatgg tggagaaaac ccgttcgccg aagtagctgg
     1501 agacctgctg caccacgtcc tgggccagcc gggtcctgga atcgtacatg gtgaacagga
     1561 taccggttat cttgaggccc gggttcatgc ccttctggac gagctggat
//
LOCUS       DAOPHV010000635         1606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_110840_length_1606_cov_4.169568, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000635 DAOPHV010000000
VERSION     DAOPHV010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110840_length_1606_cov_4.169568"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..956)
                     /locus_tag="PLQ29_12860"
     CDS             complement(108..956)
                     /locus_tag="PLQ29_12860"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPG87581.1"
                     /translation="MTALKAEGLPLVGLRYNDGWRNETRSRIDDFLSAVTHILCVVDT
                     ADTSGSWLAYIVGLARGRGTPLALYNAGPSRKTEAWLADAPLFDDPDRTVAYFRGEAV
                     AWAVKEGRRMAKAALLELGISWHAESLAQCVRDGDTKAVELFIASGFPPDVRDKAGVP
                     MLCLAARSRHVGIVELLLDKGANIDAQSDDRGYSALMDAAQQGDDRLLEILLARHADP
                     DLRSKDGQTALVLAVGRSDATAVERLLRGGADPDIADKLGLSARKYAKLFHDPRIDEA
                     FSAIAG"
     gene            1267..>1606
                     /locus_tag="PLQ29_12865"
     CDS             1267..>1606
                     /locus_tag="PLQ29_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HPG87582.1"
                     /translation="MKEHAERGPRRAVVSAAFKASVPVLLGYTSIGFAFGLVLVGLGL
                     PWWLSPLMATVVYAGAAQFMGAGLLAAGSGVVEIAILTLLLNARHAVYGLSMLERYAG
                     AGRRKPYLVFG"
BASE COUNT          293 a          493 c          495 g          325 t
ORIGIN      
        1 cccccacaag cagatccaga gtctgctgtg ctaccattac acaaccgggg aatgcattgg
       61 gcaggtggaa tatagctgaa atcgattcag agagtcaaga gtatcgctta tccggcgatg
      121 gccgaaaacg cctcgtcgat gcgcggatca tggaacagct tcgcgtactt cctggcgctg
      181 aggcctagct tatcggcgat gtcgggatcg gctccgccgc gcaggagccg ctccaccgcg
      241 gtcgcgtcgc tacgccccac ggcgagcacg agcgcggtct gaccgtcctt gctacggagg
      301 tcggggtcgg cgtgcctagc gagcaggatc tcgagcagtc tatcgtcgcc ctgctgcgcc
      361 gcgtccatca gcgcggagta gccccggtcg tcgctctggg cgtctatgtt cgcgcctttg
      421 tcgaggagca gctcgacgat accgacatgc ctcgatctgg ccgccaggca gagcatcggg
      481 acgccggcct tgtcgcgcac gtcgggcggg aagcccgagg cgatgaatag ctcgacggcc
      541 ttcgtgtcgc catcgcggac gcactgcgcc aaggactcgg cgtgccagga tatgcccagc
      601 tcgagcaggg cggccttagc catccggcgc ccttccttga cggcccatgc caccgcctcg
      661 cctctaaaat acgctaccgt ccggtccgga tcgtcgaaca gcggcgcgtc ggcgagccag
      721 gcctcggttt tgcgcgacgg cccggcgtta tataaggcta gtggcgtccc cctgccgcgg
      781 gcaaggccga cgatataggc aagccaggat ccgctcgtat cggccgtatc gacgacgcat
      841 aggatatggg taacggcgct caggaagtcg tctatccggg aacgcgtctc gttgcgccat
      901 ccgtcgttat acctgaggcc gacaagaggc aggccttcgg ccttcagtgc ggtcataacc
      961 gtacgggcgc ggtctatggt ctctggcgaa gccatcactg cgatcttcaa gcggagcctc
     1021 tgggcgatta ttgcattcca ccggccacgt tggcaaggaa catagctgca ttaccgtgtt
     1081 gacacaatat agagaatttg gatattatgc gaacaagtca atcgaacagg gtggttttcg
     1141 gctttatgca gcgtagttgc tagagcatat acccagttag tgcgatagag cttccggggt
     1201 ttacctcctt gcccgggagc tcattttttt cttgcctgcc ctcatcgcaa tcgatatagt
     1261 atctccatga aagaacatgc ggaacgaggc ccacgacgag cggtcgtatc ggccgcgttc
     1321 aaggccagcg tcccggtact cctcggatac accagcatcg gcttcgcgtt cggcctcgtc
     1381 ctggtaggtc tcggcttgcc ctggtggctc tcgccgctga tggcaacggt agtatacgcc
     1441 ggagctgcgc agttcatggg cgcgggtctc ctggccgcag gttcgggagt cgtagagata
     1501 gcgatactga ccctgctctt aaacgctcgc cacgccgtat acgggctttc catgctggaa
     1561 cggtacgcgg gggcgggccg ccgcaaaccg tacctcgtgt tcggcc
//
LOCUS       DAOPHV010000636         1602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_111277_length_1602_cov_4.098255, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000636 DAOPHV010000000
VERSION     DAOPHV010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111277_length_1602_cov_4.098255"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PLQ29_12870"
     CDS             complement(<1..559)
                     /locus_tag="PLQ29_12870"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPG87583.1"
                     /translation="MSKRRVAPLALLAVAMLAVIGGCATGTARNGEAPVSGTYEGTGY
                     GMQGPIKVQVTVDGGKIAAVKILSSKETPNVVKVAFERLPAQIVEHQALGLDAVCGAT
                     LASNGIKNAVADAVKKAGMDATKLREKTYKPQPKAPQTWSADVLVMGAGGAGLTAAIS
                     AAQTGASVILIEKGSVLGGNTMMAGA"
     gene            890..>1602
                     /locus_tag="PLQ29_12875"
     CDS             890..>1602
                     /locus_tag="PLQ29_12875"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPG87584.1"
                     /translation="MKYKIGSFRRWTGLGEGALRYYEDLGLISPYRDVGNDYRYYDER
                     NFLQLVQVRQLSGFDIQLGELPRVDTTVPIEDMRAILSRRRAALESEIDALYDRLARI
                     KLHETYFGQAYPDVSGIQKGNIRGIYRLFISDPAVANHPETAGIVRNWLSRMPYAHAT
                     LRIPLNELRERVDGPYSAQVGIGLLERYFNDIGDTFREPMQYSAPNTSVSGTILVDDP
                     RAIRRADLEPMLAYIDERSL"
BASE COUNT          313 a          485 c          467 g          337 t
ORIGIN      
        1 cggcgccggc catcatggtg ttcccgccca ggacggagcc cttctctatc agtatgaccg
       61 aggcgccggt ctgggcggcg ctgatggccg cggtgagccc ggctcctccg gctcccatga
      121 ccagtacgtc ggccgaccag gtctggggag ccttgggctg gggcttatag gttttctccc
      181 ggagcttcgt cgcgtccatg ccggctttct tgacagcgtc ggcgacggcg ttcttgatgc
      241 cgttgctggc cagcgtcgct ccgcagaccg cgtccaggcc gagcgcctgg tgctccacga
      301 tctgggcggg gaggcgctcg aacgctacct tgacgacgtt cggcgtttcc ttgctgctca
      361 gtatcttgac ggcggctatc ttcccgccat ccaccgtgac ctgtaccttg atgggaccct
      421 gcattccata gccagtacct tcataggttc cagataccgg cgcttcgccg ttcctagccg
      481 taccggtggc gcatccgcct atgactgcca acatggccac ggccagaagc gctaacggcg
      541 ctacccttct cttgctcatc gtgcatacct ccatagggga ataccccatc cgaaacggta
      601 cacccgtagg cgactgtagg gtcaatctct aaaaatatat aaatatcttt tataatcgga
      661 tcctacggtc gacaggtcgc ttctacttga tcgcgactat tgatcgcgag tcggcataga
      721 gcagacagtc gatgcctaaa gcattataat gtatcgagat gctactgccg gcttcggtgt
      781 tcgtgatcac gccgcatggt ccggctcgga tcgcgcggtc tggctttacg gcgaacctta
      841 aagcggctac ggggttatgg gcgactacat tgaaacatgg agcaacgcga tgaagtacaa
      901 gataggttcg ttcaggcgat ggaccggatt gggcgagggg gcgctgcggt actacgagga
      961 tcttggcttg atcagcccgt accgcgatgt cggtaacgac tataggtatt acgatgaacg
     1021 aaacttcctg cagctggtgc aggtccggca actgagcggc ttcgatatcc agcttggcga
     1081 gctccctcgg gtagatacga ccgtgcccat cgaggatatg cgcgcgatac tgtcccggag
     1141 gcgagcggcc ctggagtcgg agatcgacgc gctatacgat cgcctggcca ggatcaagtt
     1201 gcacgaaacc tacttcggcc aagcctatcc ggacgtgtcg ggcatacaga agggtaatat
     1261 ccgaggcatc tacaggctgt tcatctccga tcccgcggtg gcgaaccatc ccgagaccgc
     1321 cggaatcgtc aggaactggc tatcgagaat gccgtacgcg catgcgacgc tacgcatacc
     1381 tctaaacgag ctacgtgagc gcgtcgacgg cccctattcg gcccaagtcg ggatcggact
     1441 gctcgaacgg tacttcaacg acatcggcga tacgttccga gagccgatgc agtactcggc
     1501 tccgaacacc agcgtatcgg gtactatcct cgtcgacgac cctcgagcca tacgccgcgc
     1561 cgatctcgag ccgatgctcg cctatattga cgagcgcagc ct
//
LOCUS       DAOPHV010000637         1601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_111376_length_1601_cov_4.150065, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000637 DAOPHV010000000
VERSION     DAOPHV010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111376_length_1601_cov_4.150065"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLQ29_12880"
     CDS             <1..199
                     /locus_tag="PLQ29_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87585.1"
                     /translation="MANHGLVACGRNLQEALLCAQIAEKGCRAFVEAEFLGGAKSIGR
                     FEAAVMHQVYLKKYSKGKGER"
     gene            196..>1601
                     /locus_tag="PLQ29_12885"
     CDS             196..>1601
                     /locus_tag="PLQ29_12885"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPG87586.1"
                     /translation="MKGDELFVVAYDIGTTGAKTCLYRFDRAGVALAAAASSEYGIRF
                     LPNGGAEQNPDDWWKAICVGTREVLSRSGRRADEIRAAAFCCQMQGLVLVDGSGRAVR
                     PAMSYMDQRGTEQKRRGLEEGALKVAGMDARKLLPSLAVTGGVSASAKDPVWKYHWVR
                     DHEPEAFERAKRWLDVKEYLVAKATGRFAMTPDSANATFMYDTRPTRLDGRGLGSWSE
                     AMCRLFDVDRAMLPEVVAPTEVVGPVLPAAAAELGLEAGTPVFGGGGDLSLVGLGSGA
                     VEPGETHVYMGTSGWVSAVTDRRVVDTNAMIASVMGARAGLYNYMSEQETSGKCVEWV
                     RDHLVLDEVGAYLGARNAAEDPDARYASLYELLDETIESVAPGADGVMFAPWLHGNRS
                     PFEDPAARGAFFNIGLGSGKRSLVRAVSEGVAMHNRWQLESIRRKTAARGPIRFVGGG
                     ARSRAEARIMADVLGEAI"
BASE COUNT          264 a          535 c          568 g          234 t
ORIGIN      
        1 gatggccaat cacggcctcg tcgcctgcgg ccgcaaccta caagaagcgc tactctgcgc
       61 ccagatcgcc gagaaaggtt gccgcgcctt cgtcgaggcg gagttcctcg gcggggccaa
      121 gagcatcggc cgcttcgaag ccgcggtgat gcatcaggta tatttaaaga aatactcgaa
      181 aggcaaggga gagcgatgaa gggcgacgaa ctgttcgtcg tcgcctacga cataggcacc
      241 accggcgcca agacctgcct ctaccgcttc gaccgcgccg gcgtcgcgct ggcggcggcc
      301 gcgtcgtcgg agtacggtat acgcttcctg ccgaatggcg gcgccgagca gaatcccgac
      361 gactggtgga aagccatctg cgtcggcacg cgcgaggtgc tgtcccgctc cggccgccgc
      421 gccgacgaga tcagggccgc cgcgttctgc tgccagatgc agggtctcgt gctggtcgac
      481 ggctcgggcc gagcggtacg cccggcgatg agctacatgg accagcgcgg taccgagcag
      541 aaacggcgcg gactcgagga aggcgcgctc aaggtggccg gcatggacgc tcgcaagctg
      601 ctgccttcgc tcgcggtcac cggcggagtc tcggccagcg ccaaggaccc ggtttggaag
      661 taccactggg tcagggacca cgagcccgag gccttcgagc gcgccaaacg ctggctcgac
      721 gtcaaggagt acctcgtggc caaggccacc ggccgcttcg cgatgacgcc cgattcggcc
      781 aacgccacct tcatgtacga cacgaggccc acgaggctcg atgggcgcgg gctcggctcc
      841 tggagcgaag ccatgtgccg cctgttcgac gtggaccgcg ccatgctgcc ggaggtggtg
      901 gcaccgaccg aggtagtcgg gccggtgttg cccgcggccg cggccgagct gggcctggag
      961 gccggtacgc cggtcttcgg cggcggcggc gacctgtcgc tcgtcggact gggctccggc
     1021 gcggtcgagc cgggcgagac gcacgtgtac atgggcacct ccggctgggt atcggcggtg
     1081 acagatcgcc gcgtcgtcga cacgaacgcg atgatagcgt cggtcatggg cgcgcgggca
     1141 gggctctata actacatgtc ggagcaggag acctcgggca agtgcgtcga atgggtacgc
     1201 gaccacctgg tgctcgacga ggtcggcgcc tacctcggcg cgaggaacgc cgcagaggac
     1261 ccggacgccc gctatgcctc gctctacgag ctactcgacg agacgatcga atcggtagcg
     1321 ccgggcgccg atggcgtgat gttcgcgccc tggctccacg gcaaccgctc gcccttcgag
     1381 gacccggccg ctcgcggcgc gttcttcaat atcggcctcg gctcaggcaa gcgctcgctc
     1441 gtgcgcgccg tgtccgaagg cgtcgccatg cacaaccgct ggcagctcga gtcgatacgg
     1501 cgcaagaccg ccgcccgcgg ccccatacgc ttcgtgggcg gcggcgcccg gtcgcgggcc
     1561 gaggcgagga tcatggcgga cgtgctcggc gaagccatcg a
//
LOCUS       DAOPHV010000638         1600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_111464_length_1600_cov_5.386408, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000638 DAOPHV010000000
VERSION     DAOPHV010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111464_length_1600_cov_5.386408"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(334..1395)
                     /locus_tag="PLQ29_12890"
     CDS             complement(334..1395)
                     /locus_tag="PLQ29_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HPG87587.1"
                     /translation="MNKFVKRYLVDALGGMALGLFSTLIIGLILKQVGGFFPGTAPGR
                     FLVGLGNLATVLTGAGIAAGVAHSLGSPRLVIYASLLCGLVGAYASRFVDGSLFSAAG
                     VALSGPGDPLGALVAALAGAELGRLVSGKTRVDIIVTPAVTIVAGCLAGALLGPTLSQ
                     GSIALGGIIRYATELRPFLMGVVISVCMGLFLTLPISSAAIGIILGLSGLSAGAATAG
                     CSAQMIGFAVISFRDNGWNGLFAQGLGTSMLQMPNIVRNWRIWIPATAASAITGPLAT
                     TVFGMMNLPAGSGMGTSGLVGPLLAWQAMTGAGMGAPRVLFSILMVCLVLPALLSYAF
                     YALMRKKGWIRDGDMKLSS"
BASE COUNT          297 a          507 c          579 g          217 t
ORIGIN      
        1 acggtgttca ggagcaggtc gtgggccgtc agcgtgatcg tgttgatcag gttcttggcc
       61 cccatgactg agcccaggta gccgaagcag gcgataagga cgacggacgt gacgaggccc
      121 tcgatgagcc gcttgttcgc ggacggggcc ttggtggctg gcatgatttc ctccgtaagt
      181 gatcgcggag cgagtatcct cggaaggcga gacgcgcgtc aatcgctacg tcggtatcga
      241 tagaaaagaa cgctcgcgcc cgggtcccat cccaagggcc gcggagccga tagcggcggc
      301 cggctgacgc ctagggccgc cgctatcgtc gccctaggaa ctcagtttca tgtcgccgtc
      361 gcgtatccag cccttcttgc gcatcagcgc gtagaaggcg tacgacagca gggccgggag
      421 cacgaggcag accatcagga tcgagaacag gacgcgcgga gcgcccatac cggccccggt
      481 catcgcctgc caggccagga gcgggccgac gagacccgag gtgcccatgc ccgagccggc
      541 cggcaggttc atcatgccga agaccgtggt ggcgagcggc ccggtgatcg cgctcgcggc
      601 cgtggcgggt atccagatcc tccagttgcg cacgatgttc ggcatctgca gcatcgaggt
      661 tccgaggccc tgggcgaaca gaccgttcca gccattgtcc ctgaagctga tcacggcgaa
      721 gcctatcatc tgggccgagc agccggcggt ggcggccccc gctgacagcc ccgacagccc
      781 gaggatgatg ccgatggcgg ccgagctgat cggcagggtc aggaagaggc ccatgcagac
      841 cgagatgacg acgcccatca ggaagggcct gagctcggtg gcgtagcgga tgatgccacc
      901 gagcgcgatg gagccctggg acagcgtcgg gccgagcagg gcgccggcca ggcagccggc
      961 gacgatggtc acggcgggcg tgacgatgat gtcgacgcgg gtcttgcccg agacgaggcg
     1021 accgagttcc gcgccggcga gcgccgcgac gagggcgccg agcgggtctc cggggccgga
     1081 cagggccacg ccggccgccg agaatagcga gccgtccacg aagcgcgaag cgtaggcgcc
     1141 gacgaggccg cagaggaggc tggcgtagat cacgagcctt gggctgccga gcgagtgcgc
     1201 cacgccggcc gcgatgcccg cgccggtcag caccgtggct aggttgccga ggccgacgag
     1261 gaagcgaccc ggcgcggtgc ccgggaaaaa gccgccgacc tgtttgagta tcaggccgat
     1321 gatcagggtg gagaacaggc ccaaggccat gccaccgagc gcgtccacga ggtaacgctt
     1381 gacgaatttg ttcatggcgc gtagcgctcc atgcggtacc gatccatgcc caggcgggag
     1441 acgggccgga tctttcggac gggacacgca catagctccg tcagggagcg ttggtttgct
     1501 ccaggagact ccggtaccat gccgaccata cccgtccccg ccgcccgcgt caagcgggcg
     1561 aacacgcgtc aagcgggcga acacgcgtca agcgggcgaa
//
LOCUS       DAOPHV010000639         1596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_111870_length_1596_cov_3.855938, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000639 DAOPHV010000000
VERSION     DAOPHV010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111870_length_1596_cov_3.855938"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..>1596)
                     /gene="ftsH"
                     /locus_tag="PLQ29_12895"
     CDS             complement(177..>1596)
                     /gene="ftsH"
                     /locus_tag="PLQ29_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPG87588.1"
                     /translation="KAFSFGKSRAKRYQESDRKITFADVAGQKEAKYELLEVVDYLKN
                     PQKFVKMGARIPKGVLLVGMPGTGKTLLAKAVAGEADVPFFHMSGSDFVEMFVGVGAS
                     RVRDLFDQGRKSAPCILFIDELDAVGRTRGAGYGGGHDEREQTLNQMLVEMDGFDTKD
                     GVIILAATNRPDVLDPALLRPGRFDRQVVVAMPDIQEREDILKIHMAKVPMAEGVDAQ
                     RLSRATPGMSGADLANMVNEATLFAVRRGRNVVEMQDFEEARDKIMMGIARTSMVISE
                     EEKKATAYHEAGHALLHYYLPNADPLHKVTIVPRGRALGVAFSLPAQDAYSRARGWIR
                     DRIVISYGGYAAETIVYGETTTGTAQDLKQATDLARKMACEWGMSEELGAVAYGQEDE
                     PIFIGKEIAQHKDYSEDTAMRIDRAVFNILEEAKKKAFDILTEHRDQLLSLAEELIRK
                     ETLLDDDIKAMFGFKSPEANEA"
BASE COUNT          239 a          519 c          527 g          311 t
ORIGIN      
        1 gcagggccag ggcctggtcg gtatgcccga gcgccagggc cgcgacggcg ccctgggtca
       61 gcgaggcgca gatggcggcg tccctggcgg tcgagtcagc cgccgcgggc agcgtcgaga
      121 tgagcaatag cgacgcggcg agggctagcg agatggctgc cccgccgcgt cccggcctag
      181 gcctcgttgg cctccgggga cttgaaaccg aacatggcct tgatgtcgtc atcgagcagg
      241 gtctccttgc ggatcagctc ctcggccagg ctgagcagct ggtcacggtg ctcggtcagg
      301 atgtcgaacg ccttcttctt ggcttcctcg aggatattga acaccgcgcg gtcgatgcgc
      361 atcgccgtat cctccgaata gtccttatgc tgggctatct ccttgcctat gaagatcggt
      421 tcgtcctcct gcccgtacgc gacggctccg agctcctcgg acatgcccca ttcgcaggcc
      481 atcttcctgg ccaggtcggt cgcctgcttc aggtcctggg ccgtgccggt cgtcgtctcg
      541 ccgtacacta tagtttcggc cgcgtagccg ccgtacgaga tgacaatcct atccctgatc
      601 cagccgcgag cgcgcgagta agcgtcctgg gccggtagcg agaaggccac gccgagggcg
      661 cgcccccgtg ggacgatggt caccttatgg agcggatcgg cgttcggcag gtaatagtgg
      721 agcagcgcgt ggccggcctc atggtaggcc gtcgctttct tctcctcctc ggagatgacc
      781 atcgaggtgc gggctatgcc catcatgatc ttgtcgcggg cttcctcgaa gtcctgcatc
      841 tcgacgacgt tccgaccccg cctgacggcg aatagcgtcg cctcgttaac catattggcc
      901 aggtcggcgc cgctcatgcc cggggtggcg cgtgaaagcc gctgggcgtc gacgccctcg
      961 gccatcggca ccttggccat gtgtatcttg aggatatcct cgcgctcctg gatgtcaggc
     1021 atggcgacga cgacctggcg gtcgaagcgg ccgggcctga gcagggccgg gtcgaggacg
     1081 tccgggcggt tggtcgccgc gaggatgatg acgccgtcct tcgtgtcgaa gccgtccatc
     1141 tcgacgagca tctggttgag cgtctgctcg cgctcgtcgt gcccgccgcc gtagccggcg
     1201 cctcgcgtgc ggccgacggc gtcgagctcg tctatgaaga ggatgcacgg cgcgctcttg
     1261 cggccctggt cgaagaggtc gcgtacgcgg ctggcgccga cgccgacgaa catctcgacg
     1321 aagtccgatc cggacatatg gaagaacggc acgtcggcct cgccggccac ggccttggcc
     1381 aggagggttt taccggttcc gggcatgccg acgagcaaca cacccttagg tatgcgcgca
     1441 cccatcttca cgaatttctg cgggttcttg agatagtcga ccacctcgag gagctcgtac
     1501 ttggcctctt tctggcccgc cacgtcggcg aaggtgatct tacggtcgct ctcctggtaa
     1561 cgcttggccc gactcttccc gaagctgaag gccttg
//
LOCUS       DAOPHV010000640         1596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_111890_length_1596_cov_3.117456, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000640 DAOPHV010000000
VERSION     DAOPHV010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111890_length_1596_cov_3.117456"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1205)
                     /locus_tag="PLQ29_12900"
     CDS             complement(165..1205)
                     /locus_tag="PLQ29_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG87589.1"
                     /translation="MYKWYALKRILRGVFMFALLMFVFSFLFNQVNEQTQRSQINEQV
                     KMESMRLKGMKPEQITAWRDNRTDQLIKMYKLDRPLMERVLFRAVNTVTFNFGKSTVI
                     KSSKGEQDVLDILMEALPRTMILFTSAIVFELIIGIALGLKKAQKPGGKLDKSTSFIT
                     MIIYGMPSWWLGMMLILLFVYVLKLFPSGGVNSVPTPAGFAFVLDRIWHMALPILTLV
                     ILGFWSLSFVIRNLVLSTLQEDYIMSARARGIPESKVLFGHTLRTAAPPIVTMALLSL
                     LASLSGAIIFEGIFSWPGLGNLYWVAVQQNDIPVLMGDLAITTGIYQAGLVFLDLIYG
                     FLDPRIKVGGKA"
     gene            complement(1342..>1596)
                     /locus_tag="PLQ29_12905"
     CDS             complement(1342..>1596)
                     /locus_tag="PLQ29_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87590.1"
                     /translation="PSGSPATSASTPLDKKAKVELRLQMDDGSEKVYAAKYLKPGSFS
                     ATIPAKDLAALKSGKSYTVVIISSLASEAPSVVPTSLVLF"
BASE COUNT          340 a          444 c          511 g          301 t
ORIGIN      
        1 gatgttgcgc cagttgccgt ccacgtcctt gaacggcagg aacaccggct cgaacagcac
       61 gacgaccagc gagaggacga gtatcccgag gccgatcagg ccggaggtct ccttgcggaa
      121 atcgtcccag aattccttga agcggtagcc cacgtctttc atgctcatgc cttgcctcct
      181 accttaatgc gcggatccag gaagccgtag atgaggtcca ggaagacgag gccggcctgg
      241 tagatgccgg tcgtgatggc gaggtctccc atcagcaccg ggatgtcatt ctgctggacc
      301 gccacccagt acaggttgcc gagtccgggc caggagaaga tgccttcgaa gatgatcgcg
      361 cccgagaggg acgccagcag ggacaggagg gccatcgtga cgataggcgg ggccgcggtg
      421 cgcagggtgt ggccgaagag caccttgctc tcggggatgc cgcgggcgcg ggcgctcatg
      481 atgtagtctt cctgcagggt gctgagcacc aggttcctga tgacgaacga caggctccag
      541 aagccgagta tgacgagggt caggataggc agggccatgt gccagatacg gtcgagcacg
      601 aaggcgaagc ccgcgggcgt cgggacggag ttgactccgc cggacgggaa cagcttgagc
      661 acgtagacga acagcagtat cagcatcatg ccgagccacc acgacggcat gccgtagatg
      721 atcatcgtga tgaagctggt cgacttgtcg agcttaccgc ccggcttctg agccttcttg
      781 aggccgagag cgatgccgat gatcagctcg aagacgatag ccgacgtgaa cagtatcatc
      841 gtccgcggaa gggcttccat gagtatgtcg agcacgtcct gctcgccctt cgacgacttg
      901 atgacggtcg actttccgaa attgaacgtg acggtgttga cggcgcggaa cagtacgcgc
      961 tccatcaagg gccggtccag cttatacatc ttgatgagct ggtccgtccg gttatcccgc
     1021 caagccgtga tctgctcagg cttcatgccc ttgagccgca tggactccat cttgacctgc
     1081 tcgttgatct gcgatcgctg agtctgctcg ttcacctggt tgaacaggaa cgagaagacg
     1141 aacatcagca acgcgaacat gaacacgccg cggagaatgc gctttagcgc ataccactta
     1201 tacatagtac cacctaaaag aactcgattg gatcagacga cgttttcttg ttttgattgt
     1261 tgaaaaaccg tttcacgtat caagaagccg tctgaaccga aagcggcgcc ccgaagggcg
     1321 ccgctgaacc tctttagagg attagaacag gacgaggctg gtcgggacta ccgacggggc
     1381 ctcggaggcg agagaggaga tgatgaccac ggtgtaggac ttgccggact taagcgcggc
     1441 caggtccttg gcggggatcg tcgcggagaa cgaaccgggc ttcaggtact tcgcggcgta
     1501 gaccttctcg ctgccgtcgt ccatctggag cctgagctcg accttggcct tcttgtcgag
     1561 gggggtcgac gcggaggtag cgggcgaccc gctggg
//
LOCUS       DAOPHV010000641         1593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_112182_length_1593_cov_3.099480, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000641 DAOPHV010000000
VERSION     DAOPHV010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112182_length_1593_cov_3.099480"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1593
                     /locus_tag="PLQ29_12910"
     CDS             <1..>1593
                     /locus_tag="PLQ29_12910"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPG87591.1"
                     /translation="KVNLARLVELRKRVEGFMDMASPPVARPSLDGSADGEPADLDVA
                     GKRLDAIAADINGLRERQKDLQDQILKLGEIRRQLEAFGDLKAGAAASSAYSFLSIRA
                     GLMPADVADGLHEALEGIPSVVLPSSGRDAAAASQARRHEAVGVMLVTLKRDAARVEP
                     ILERLGWEDARLPESAGQGNAEALETIDRKIDELRARQAACVDDLNAYFASIGSGLGS
                     MWAALRTEELIATVRSGFARTDRTVLFSGWIPAEKAAAVEAGIRKAASGACSVEWLEP
                     GKGEAARLEAPVEMRNPKPLKPFEGLVRNYAVPEYGSVDPTPFVAVAYLCMFGLMFGD
                     AGHGLVVLLVGLLGLASARRSGKPGDLYRVIAYCGGAAIVAGVLFGSYFGMQWLPPLW
                     FDYHGIVNGHEAAGSVRTLYDILGLTIRFGMVVLGLGFAINWVNLFRKRDWFTLLLDK
                     AGVIGGWIYGAGAWAAFYYVRHDYRELPPVGVLALLLGLPTMILAFKAPLEFVIHGRA
                     SGKRFKPGLIVDFVMEWIVEVL"
BASE COUNT          231 a          536 c          556 g          270 t
ORIGIN      
        1 ccaaggtcaa cctggccagg ctcgtcgagc tgcgcaagcg tgtcgagggc ttcatggaca
       61 tggcctcacc cccggtggcc aggccgtcgc tcgacggctc cgcggacggg gagcccgccg
      121 acctcgacgt cgccggcaag cgcctggacg ctatagcggc cgacatcaac ggcctgcggg
      181 agcgccagaa ggacctgcag gaccagatcc tgaagctcgg cgagatacgc aggcagctcg
      241 aggccttcgg cgacctcaag gccggggcgg ccgcgtcgtc ggcgtattcg ttcctgtcca
      301 tacgcgccgg actcatgccg gcggacgtcg cggacggcct ccacgaggcc ctcgagggca
      361 taccgtcggt ggtgttaccc tcgtccgggc gggacgccgc ggccgcctcg caggctcgtc
      421 gacacgaggc cgtcggcgtc atgctcgtta cgctcaagcg cgacgcggcg cgggtggagc
      481 ccatcctcga gcggctcggc tgggaggacg cgaggctacc cgagtcggcc ggacagggca
      541 acgccgaggc gctcgagacg atagaccgca agatagacga gctccgggcc aggcaggcgg
      601 cctgcgtaga cgacctgaac gcctatttcg cgtcgatagg ctccggcctc ggctcgatgt
      661 gggccgccct gaggaccgag gagctcatcg cgacggtccg ctcgggcttc gcgcgtaccg
      721 accgtaccgt gctgttctcg ggctggatcc cggccgagaa agccgccgct gtcgaggcgg
      781 gtatccggaa agcggcctcc ggcgcctgct cggtcgagtg gctggagccc ggcaagggcg
      841 aggccgccag gctcgaggcg ccggtggaga tgcgcaaccc gaaacccctg aagcccttcg
      901 agggcctcgt gcgcaactat gcggtgcccg agtacggaag cgtcgacccg acccccttcg
      961 tcgcggtcgc gtacctatgc atgttcggcc tgatgttcgg cgacgctggg cacggcctcg
     1021 tcgtgctgct cgtcggcctc ctcggactgg cctcggcgag gcgatccggc aagccgggcg
     1081 acctgtaccg cgtcatcgcg tactgcggcg gcgcggccat cgtcgctggc gtgttgttcg
     1141 gctcgtactt cgggatgcag tggctgccgc cgctctggtt cgactatcac ggcatcgtca
     1201 acggccacga ggccgcgggc tcggtacgca ccctgtacga tatcctgggt ctcaccatac
     1261 gcttcggcat ggtcgtgctc gggctcggct tcgcgatcaa ctgggtcaac ttgttcagga
     1321 agcgggactg gttcacgtta ctgctcgaca aggcgggcgt catcggtggc tggatctacg
     1381 gcgccggcgc ctgggccgcg ttctactacg tgcggcacga ctaccgggag ctgccgccgg
     1441 tcggggtcct ggccctgctg ctcggccttc ccactatgat cctggccttc aaggctcccc
     1501 tggaatttgt cattcacggc cgagcctcgg gcaagcgctt caagccgggc cttatcgtcg
     1561 actttgtcat ggagtggata gtcgaggtgc tgg
//
LOCUS       DAOPHV010000642         1590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_112507_length_1590_cov_3.224104, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000642 DAOPHV010000000
VERSION     DAOPHV010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112507_length_1590_cov_3.224104"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="PLQ29_12915"
     CDS             complement(<1..1160)
                     /locus_tag="PLQ29_12915"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPG87592.1"
                     /translation="MHVLMVTSEATPYAKSGGLADAVSALSKALRRLGHDVRVVMPRY
                     GSVPVEGLSRREGELVVEMGGSVERALVYKGLLPDSDLPVYLIDHPGFFGRDGLYGDK
                     TEPDYADNPRRFAFLSRAAFRLCDALRWKPDVFHAHDWPGGFLPAYLRYAEPGYASSA
                     SLLSIHNLGYQGIYPKDAYPYFGLPWELFHGAGFEYYDSINALKAGIVCADGLSTVSP
                     TYAREIQTPEFGCTLDGLLRSRGADLVGILNGVDAQDWDPAHDRYLPARYSTDDLRGK
                     AACKAALQREFGLPEDPDKPLFGMVSRLTDQKGIGELFGPTYGCVEGMCRDMDLQFVV
                     LGSGEPWCERAIVELAGRLPNFGSRIGYSEPAAHLIEAGSDFFMMPSRYEPCG"
     gene            complement(1283..>1590)
                     /locus_tag="PLQ29_12920"
     CDS             complement(1283..>1590)
                     /locus_tag="PLQ29_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPG87593.1"
                     /translation="RLSEDGKRRVMAMKSTTDGFEIAEEDLRIRGPGEITGTTQAGAL
                     RLAFADPVRDAELLEAAREDAAALLATDPGLVGQEGGIVRAVLERASPFSERTAATG"
BASE COUNT          274 a          515 c          553 g          248 t
ORIGIN      
        1 ccgcacggct cgtagcggct cggcatcatg aaaaagtcag agcccgcctc gatcaggtgg
       61 gccgcgggct cggagtagcc tatcctcgag ccgaagttcg gaagtcgacc cgccagctcg
      121 acgatggcgc gctcgcacca gggctcgccc gagcccagga cgacgaactg caggtccatg
      181 tcccggcaca tcccctcgac gcagccgtag gtggggccga agagctcgcc gatgcccttc
      241 tggtcggtca agcgcgacac catgccgaag aggggcttgt ccggatcctc gggtaggccg
      301 aattcacgct gcagggccgc cttgcaggcc gccttgccgc ggaggtcgtc ggtcgagtag
      361 cgggcaggca ggtagcggtc gtgggccgga tcccaatcct gggcgtccac cccgttgagt
      421 atgccgacga ggtcggcgcc ccgggagcgc aggaggccgt ccagggtgca gccgaattcg
      481 ggcgtctgga tctcgcgcgc gtaggtaggc gaaacagtcg acaacccgtc ggcgcagacg
      541 atgcccgcct tgagcgcgtt gatcgagtcg tagtactcga agccggcgcc gtggaacagc
      601 tcccatggca ggccaaagta tggataggcg tccttggggt agataccctg gtaaccgagg
      661 ttatggatgc tcaggaggct ggccgatgag gcgtagccag gctccgcgta gcgcagatag
      721 gccggcagga aaccgcctgg ccaatcgtgg gcgtggaaga cgtcgggctt ccagcgtagc
      781 gcgtcgcata gcctgaaggc ggccctggat aggaacgcga agcggcgcgg gttatcggcg
      841 taatcgggct cggttttgtc gccgtataat ccgtcgcggc cgaagaagcc ggggtgatcg
      901 atgaggtaca cgggcaggtc ggaatcgggc aggaggccct tgtagacgag cgctcgctcg
      961 accgagcccc ccatctcgac gacgagctcc ccctcccgcc gagaaagccc ctcgacggga
     1021 accgagccgt agcggggcat cacgacgcgg acatcgtgcc cgaggcggcg cagggccttg
     1081 gataaggccg agacggcgtc cgcgagcccg ccagacttgg cgtagggcgt cgcctcgctg
     1141 gttaccataa gcacgtgcac aggccggctc ctcgtatgcg caaaacctgc gcggtattag
     1201 tcgttctcag tgcttaacaa acaagcagta tactaagata cgggcctgcg ggcaacgccg
     1261 cgacggatgg cgccagccgc attcagccgg tggcggccgt acgctccgag aaggggctgg
     1321 ctcgctcgag caccgcgcgg actatcccgc cctcctgccc gacgagccct ggatctgttg
     1381 ccaggagggc cgcggcgtcc tctctggccg cctcgagcag ctcggcgtcg cgcactgggt
     1441 cggcgaaggc cagcctcagg gcgccggcct gggtcgtgcc ggtgatctcg ccggggcctc
     1501 ggatgcgcag gtcctcctcg gctatctcga agccatccgt cgtcgacttc atcgccatca
     1561 cgcggcgctt gccgtcctcg gacaacctgg
//
LOCUS       DAOPHV010000643         1590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_112546_length_1590_cov_2.255375, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000643 DAOPHV010000000
VERSION     DAOPHV010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112546_length_1590_cov_2.255375"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..81)
                     /gene="rplC"
                     /locus_tag="PLQ29_12925"
     CDS             complement(<1..81)
                     /gene="rplC"
                     /locus_tag="PLQ29_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692855.1"
                     /note="binds directly near the 3' end of the 23S rRNA,
                     where it nucleates assembly of the 50S subunit; essential
                     for peptidyltransferase activity; mutations in this gene
                     confer resistance to tiamulin; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPG87594.1"
                     /translation="MIGLIGKKIGMTQVFDDAGKLMPVTVI"
     gene            complement(126..431)
                     /gene="rpsJ"
                     /locus_tag="PLQ29_12930"
     CDS             complement(126..431)
                     /gene="rpsJ"
                     /locus_tag="PLQ29_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPG87595.1"
                     /translation="MKDRIRVRLRGFDVRLIDDSAKSIVTTVQRAGAKVSGPIPLPTK
                     ISKYTVLRSPHVHKKAREQFEMRTHKRLIDILSPTPDVMDALMKLELPAGVDVEIKQ"
     gene            complement(809..>1590)
                     /gene="ugpE"
                     /locus_tag="PLQ29_12935"
     CDS             complement(809..>1590)
                     /gene="ugpE"
                     /locus_tag="PLQ29_12935"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HPG87596.1"
                     /translation="LLFPIILVFVASTHTSADILAAPMPMKPGPHLVENYTRAILEGN
                     KNLGASALTMMKNSLIMALGIAIGKIVVSLLAAYAIVFFKFPLRNFCFWAIFVTLMLP
                     VEVRIMPTYKVISDLGLLNSYAGLILPMIVSATAVFLFRQFFMQIPREIAEASQIDGA
                     TPMQFFRSILLPMTRTPIAAMFVIQFIYGWNQYLWPLLITTKQEYYTILIGINRMLSG
                     ADVQIEWQIVMATTLLAMIPPALIVVVMQKQFIAGMTDTEK"
BASE COUNT          347 a          460 c          449 g          334 t
ORIGIN      
        1 gatcaccgtg acaggcataa gcttgccggc gtcgtcgaag acctgggtca tgccgatctt
       61 ctttccgatc aatccaatca tcggttactc ctaagcgttg aacggtacgg tacggaaccg
      121 tccgtttact gcttgatctc gacgtccacc cctgcgggca gctcgagctt cattaacgcg
      181 tccatgacat cgggggtcgg gctcagaatg tcgatcagcc gcttatgggt gcgcatctcg
      241 aactgctcgc gggccttctt atgcacgtga ggggatcgaa gcaccgtata cttgctaatc
      301 ttggtcggca gcgggatcgg accggagacc ttcgctcccg ccctctgaac tgtcgttacg
      361 atggatttcg cactgtcgtc tataagccga acatcgaaac ccctgagcct gacacggatc
      421 ctgtctttca tgtgtcctcc aaagatcgct tgcgccggca tccatcaggc cgtaccctgg
      481 cgggcgtgcc caagaaccct gacgctcgag gtcgtggaac ctcgcgccgc cgtagcggaa
      541 cccgcttacg acggctaccc gtccttcgcg gacgccacgg cgcccagacg aatagcccgg
      601 ggattatgcc cttatacgga ataaatgtca aggctgtttg gcgactcggg aaggcgattg
      661 aatcatacag gagactaaaa ccatgaaaac acggagaaga aggacctagc tattgagaaa
      721 gaggggcgag aaccctatcc acgcccctgc tctctagatg tcccacagtg cccctgggcc
      781 tcaacggcca agattcggga gttacgtctt atttctccgt atcggtcata cccgctatga
      841 actgcttctg catgacgacg acgatgaggg ccggcggtat catcgcgagc agcgtcgtgg
      901 ccatgactat ctgccattcg atctgcacgt cggctcccga cagcatacgg ttgataccga
      961 tcagtatggt gtagtattcc tgcttcgtgg tgatcagcag cggccacagg tactgattcc
     1021 agccgtagat gaactggatg acgaacatcg cggcgatcgg cgtcctggtc atcggcaaca
     1081 ggatgctccg gaagaactgc atcggcgtgg cgccgtctat ctgcgaggct tcggctattt
     1141 cgcgcggtat ctgcatgaag aactgcctga acaggaatac cgccgtcgcg gagactatca
     1201 tgggcaggat gagccccgcg tagctgttga gcaagcccag atccgatatg actttatacg
     1261 tcggcatgat gcgcacctcg accggcagca tcaacgtgac gaagatcgcc cagaaacaga
     1321 agtttcgcag ggggaacttg aagaacacta tcgcgtaggc cgcgagcagc gacaccacga
     1381 tcttgcctat cgctatgccg agggccatga tcagcgagtt cttcatcatc gtaagggccg
     1441 aggccccgag atttttattt ccctcgagta tcgctcgagt atagttctcg accaggtgcg
     1501 gccccggctt catcggcatc ggcgcggcga ggatatccgc gctcgtatgg gtcgaggcga
     1561 cgaacaccag gatgataggg aacagcaata
//
LOCUS       DAOPHV010000644         1585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_113019_length_1585_cov_3.811765, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000644 DAOPHV010000000
VERSION     DAOPHV010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113019_length_1585_cov_3.811765"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1585
                     /locus_tag="PLQ29_12940"
     CDS             <1..>1585
                     /locus_tag="PLQ29_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPG87597.1"
                     /translation="VRDGAERYLLAESRLGAYYKEGQQPEILWRKKGSELVGIRYEPL
                     FPYFAELAAQKAFRTWTGGHVTTEDGTGIVHTAPGFGEEDHAVLKGTGVPTVCPIDAE
                     CKFTPEVSDYAGRFVKDCDKDIMERLKAEGKLAKRDQILHAYPHCWRCDHPLIYRAVS
                     SWFVKIDPIKPKFVATNQQIHWEPEHIKNGRFGKWLDGARDWAISRSRYWGNPLPIWK
                     CPDCGKTICIGSRDELEKRSGVRLSDLHKHFVDEVTMPCSCGATMSRIPDVLDCWFES
                     GSMPYAQVHYPFENKEHFESHFPADFISESLDQTRGWFYSLTVLAAALFDRPAYQNCI
                     VSGMVLAADGKKMSKSQRNYTDPMEAIDKFGADALRLYLMASPVLKADDLCFTDDGVR
                     DVVKSILLPLWSAYGFFVTYANIDGIEPGEAPIAAANPMDRWILSKCETMVRDVGAGL
                     EAYTMQAAIAPMLDFIDALNNWYIRRSRRRFWRSENDGDKVEAYSTLYRALKRLALCM
                     APFTPFITETMWRNLRREGE"
BASE COUNT          307 a          550 c          480 g          248 t
ORIGIN      
        1 cgtccgggac ggcgccgagc gctacctgct cgccgagagc cgcctcggcg cgtattacaa
       61 ggaaggccag cagcccgaga tactctggcg caaaaagggc tccgagctgg tcggcatacg
      121 ctacgagccg ctgttcccct acttcgccga actcgcggcc cagaaggcct tccgcacctg
      181 gaccggcggc cacgtaacca ccgaggacgg caccggcatc gtgcataccg cgccaggctt
      241 cggcgaggag gatcatgcgg tgctcaaggg caccggcgtg ccgacggtct gccccattga
      301 cgccgagtgc aagttcacgc cggaggtatc ggactacgcc gggcgcttcg tcaaggactg
      361 cgacaaggac atcatggagc ggctcaaggc cgagggcaag ctggccaagc gcgaccagat
      421 cctgcacgcc tacccgcact gctggcgttg cgaccatccg ctcatctacc gcgcggtgtc
      481 gagctggttc gtcaagatag acccgataaa gccaaaattc gtagcgacga accagcagat
      541 ccactgggag ccggagcata tcaagaacgg ccgcttcggc aagtggctcg acggcgcccg
      601 cgactgggcc atatcgcgca gccgctactg gggcaacccc ttaccgatct ggaaatgccc
      661 cgactgcggc aagaccatct gtatcggctc gcgcgacgag ctggagaaac gttccggcgt
      721 caggctgagt gacctgcaca agcacttcgt cgacgaggtg accatgccct gctcctgcgg
      781 ggcgacgatg agccgtatcc ccgacgtgct cgactgctgg ttcgagtccg gctcgatgcc
      841 ctacgcgcag gtccactacc ccttcgagaa taaggaacat ttcgagagcc acttcccggc
      901 agacttcatc tccgagagcc tcgaccagac gcgcggctgg ttctactccc tgaccgtgct
      961 cgcggccgcc ctgttcgacc gcccggccta ccagaactgc atcgtctccg gcatggtgct
     1021 cgcggccgac ggcaagaaga tgtccaagag ccagcgcaac tacaccgacc cgatggaagc
     1081 tatcgacaag ttcggagcgg acgcgctcag gctctacctg atggcctcgc cagtgctcaa
     1141 ggccgacgac ctgtgcttca ccgacgacgg cgtccgcgac gtggtcaaga gcatactcct
     1201 accgctatgg agcgcctacg gcttcttcgt cacctacgcg aacatcgacg gcatcgagcc
     1261 aggcgaggcg ccgatcgcgg ccgcgaaccc gatggaccgc tggatcctat ccaagtgcga
     1321 gacgatggtg cgcgacgtcg gggccgggct cgaggcctac accatgcagg ccgccatcgc
     1381 gccgatgctc gacttcatcg acgccctgaa caactggtac atccgccgct cgcgccgccg
     1441 cttctggcgc tccgagaacg acggcgacaa ggtcgaggcc tactcgaccc tgtaccgcgc
     1501 gctcaagcgt ctggccctgt gcatggcgcc gttcacgccc ttcatcaccg agactatgtg
     1561 gcgcaacctg cgccgcgaag gcgag
//
LOCUS       DAOPHV010000645         1585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_113026_length_1585_cov_3.620261, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000645 DAOPHV010000000
VERSION     DAOPHV010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113026_length_1585_cov_3.620261"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PLQ29_12945"
     CDS             <1..512
                     /locus_tag="PLQ29_12945"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPG87598.1"
                     /translation="AYSAGVPCRLYRGERHFPAAKSLSMLLSTVAFRSAAAAGCYDAL
                     LVDRRGEITEGTRTNLVYARRGDASTIYTPPTDRALEGITRRTLIAALAEAGVAVAER
                     PLSVAEALSGEYALAVTSTSSRVIQVCALLPVPGETAGATDCPRSPELASAAAVYDAY
                     LERYSMELS"
     gene            613..1557
                     /locus_tag="PLQ29_12950"
     CDS             613..1557
                     /locus_tag="PLQ29_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87599.1"
                     /translation="MKDRVAIASVLASFALSALVVAAQPAVGQPAAARGSGSRHVLVL
                     YSYSRLLPWEDRVMRGMDAALGAIALNRRPFLYEESLDAGRVGTIGDLDAWRRYLEEK
                     YRDVRLDAVITESQQAASLLLSLPELFPGAERYVLHYAEGSSLGGGNGAERRFSSSED
                     MERALRLIPTLRPGTRRVVVVTDRTVNGMARVEQIAQLADRLEGLGIDIWDTFTEEEL
                     LSRAADLPKDTAVMYLPVQRDRNGGRLVPGVVGERLAAAASVPVFTHFDSLLGSGIVG
                     GYLVSGDLLGRLMGEIAARGDMAVPVSQTGVTANFTPS"
BASE COUNT          221 a          559 c          544 g          261 t
ORIGIN      
        1 cggcctatag cgccggcgtg ccatgtcggt tgtaccgcgg cgagcggcat ttcccggccg
       61 ccaagagcct gtccatgctg ctgtcgaccg tcgcgttccg gtcggccgcc gcggccggtt
      121 gctacgacgc cctgctcgtc gacaggcgag gggagatcac cgagggaacg cggaccaacc
      181 tggtctacgc gcgccggggc gacgcctcga ccatctacac gccgccgacc gaccgcgcgc
      241 tcgagggcat cacgcggcgc accctgatcg ccgcgctggc cgaagccggc gtcgccgtgg
      301 ccgagcggcc cctgtcggtc gccgaggcgc tatccggcga gtacgcgctc gcggtgacca
      361 gcacctcgag ccgggtcatc caggtctgcg cgctactgcc ggtccccggc gagacggccg
      421 gcgccacgga ctgcccccgg tcgcccgagc tggcctccgc ggcggccgtc tacgacgcct
      481 atctggagcg ttattcgatg gagctctcgt agcgcgcggc gtcgccggcc gtttttcgtt
      541 gcgccgatct cgttcatgcc gatatactga gtatcgcgtg atgcgagccc cgcatcggaa
      601 cggagagtcg tcgtgaagga ccgagtggcg attgctagcg tcctcgcctc gttcgcccta
      661 tcggcgctcg tcgtcgccgc ccagccggcc gtcggccagc cggcggccgc tcggggctcc
      721 ggttcaaggc acgtgctcgt cctatattcg tactcccgcc tcctaccctg ggaggaccgg
      781 gtcatgcgcg gcatggacgc ggcgctcggc gccatcgcgc tgaaccgccg accgttcctc
      841 tacgaggaga gcctcgacgc cggccgcgtc gggacgatag gcgacctgga cgcctggcgc
      901 cgctacctcg aggagaagta ccgcgacgtc cgcctcgacg cggtgatcac cgagagccag
      961 caggcggcgt cgctattgct gtccttaccc gagctgttcc cgggagccga gcgctacgtg
     1021 ctgcactacg ccgagggctc gtcgctcgga ggcgggaacg gggccgagcg acgcttctcg
     1081 tcgtccgagg acatggagcg ggcgttgcgc ctcatcccga cgctacgccc cggcacgagg
     1141 cgggtcgtgg tcgtcacgga ccgtaccgtg aacggcatgg ccagggtcga gcagatcgct
     1201 cagctcgccg accggctcga aggcctcggc atcgatattt gggacacgtt caccgaggag
     1261 gagctgctct cccgagcggc cgacctcccc aaggacacgg ccgtcatgta ccttccggtc
     1321 caacgggata ggaacggcgg gcgcctcgtg cccggagtcg tcggcgaacg cctggccgcg
     1381 gcggcgtcgg tgccggtgtt cactcatttc gactcgttgc tcggatcggg catagtcggt
     1441 ggctacctgg tctccggcga cctgctgggc cgcttgatgg gagagatagc cgcccgcggc
     1501 gacatggccg taccggtctc ccagaccggc gtaaccgcta atttcacccc gtcttgacgg
     1561 gaataagggc ttgtggatcg agcgt
//
LOCUS       DAOPHV010000646         1578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_113787_length_1578_cov_3.512147, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000646 DAOPHV010000000
VERSION     DAOPHV010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113787_length_1578_cov_3.512147"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLQ29_12955"
     CDS             <1..242
                     /locus_tag="PLQ29_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87600.1"
                     /translation="TVSVVLPAASINTDMKGGHWSQIQIYVDGAIQRGQTLQLPPGRH
                     LIKITSGGLQVEGFYDIQAGVSYTIEPFMGLNLRQ"
     gene            370..810
                     /locus_tag="PLQ29_12960"
     CDS             370..810
                     /locus_tag="PLQ29_12960"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HPG87601.1"
                     /translation="MSKLERALATALAVVAVVVLTGTVYAGLSGSRARKLAREAVPAA
                     AAGRGVYDGLGRLRARTADEAVVVVDLAFPYDASDRQFREELGRRRGDLREAVVGYLS
                     SRRADELGSESEPAVKAALRDTLNAILSLGSVEELYFAEFNVIQ"
     gene            841..1242
                     /locus_tag="PLQ29_12965"
     CDS             841..1242
                     /locus_tag="PLQ29_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87602.1"
                     /translation="MTKAIGVFVPVLACLLTIACPMSVPKFSLVGYSYHRADSGSRDV
                     ASGDTVNFDVVLEYEAEGIIQIDFYWTYGIDDEVNSNYSEGQGPMYSKSGTDQSLAGS
                     YTFSTGFTGVNWVTVKGVAFKQDGEVITDKV"
     gene            1258..>1578
                     /locus_tag="PLQ29_12970"
     CDS             1258..>1578
                     /locus_tag="PLQ29_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87603.1"
                     /translation="MGPYIDVEPRNLSGVSSLERITVRIADGGSDAGVDYDRTSFTLR
                     DGDGTLVPLASPTHDGKGTYTASLVRTPSVGTYSVSVIGYDGIGNPAEFKSTFELADT
                     NAVAP"
BASE COUNT          300 a          469 c          508 g          301 t
ORIGIN      
        1 gcacggtgtc ggtggtcctg ccggccgcga gcatcaatac ggatatgaag ggcgggcact
       61 ggagccagat ccagatctac gtcgacgggg ccatccagcg cggccagacc ctccagctgc
      121 cgcccgggcg tcacctgatc aagatcacct ccggcggcct ccaggtcgag ggcttctacg
      181 acatccaggc cggcgtgagc tacacgatcg agccgttcat ggggctgaac ctgaggcagt
      241 aatcgatggc cgcgacgcgg ccgatcggaa cgcctttagc cgccgggcct cgccccggcg
      301 gtttttttat tgtctatcgt cgaccgtatc cactcggcgg aaatgacgta aacggggtat
      361 actccgccca tgagcaagct tgagcgggcg ttggcgacgg ccctggctgt cgtcgcggtg
      421 gtggtgctga cgggcacggt ctacgcgggc ctgagcggct cgagggccag gaagctggcg
      481 cgcgaggccg tgccggcggc cgccgcgggg cggggcgtct acgacgggct gggccgcctg
      541 agggccagga ccgccgacga ggccgtggtc gtggtcgacc tggcctttcc gtacgacgcg
      601 tccgaccggc agttccgcga ggagctgggc cggcgcaggg gcgacctgcg cgaggcggtc
      661 gtcggctacc taagctcgag gcgcgccgac gagctcggct ccgagagcga gccagccgtc
      721 aaggccgccc tgcgcgatac cctcaacgcc atcctgtctc tcggctccgt cgaggaactg
      781 tacttcgcgg aattcaacgt gatacaataa cttggcttgc atgctaaaag gagatccgat
      841 atgacgaagg ccatcggcgt gtttgttccg gttctagctt gccttctaac aatcgcttgc
      901 ccgatgtctg tccccaagtt ctctctcgtg ggctattcat atcaccgcgc cgattctggt
      961 agcagagacg tcgcctcggg agataccgtc aacttcgacg tcgtgctcga gtacgaggcc
     1021 gaaggcatca tacagataga cttctactgg acgtacggaa tagatgacga ggtcaattct
     1081 aactatagcg aagggcaggg gccgatgtat agcaagtcgg gtacggatca gagccttgcc
     1141 ggatcatata cgttcagtac cggttttacc ggtgtaaact gggtcaccgt caagggcgtc
     1201 gccttcaagc aggacggtga ggtgatcacc gataaggtct gattcaatat agacaacatg
     1261 ggaccctata tcgatgtgga gcccaggaac ctctcgggcg tatcgagcct cgagcgcata
     1321 accgtccgta tcgcggacgg gggatcggac gcgggcgtcg actacgaccg gacttcgttc
     1381 acattgcggg acggagacgg caccctggtt ccgctcgcta gtcctacgca cgacggcaag
     1441 ggaacgtata cggcttcgct cgtacggaca ccgtctgtcg gtacgtattc ggtaagcgtc
     1501 atcggctacg atggtatcgg taatccggca gagttcaaat ccacgttcga attggccgat
     1561 acgaacgctg tcgctccc
//
LOCUS       DAOPHV010000647         1575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_114130_length_1575_cov_3.886842, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000647 DAOPHV010000000
VERSION     DAOPHV010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114130_length_1575_cov_3.886842"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1020)
                     /locus_tag="PLQ29_12975"
     CDS             complement(124..1020)
                     /locus_tag="PLQ29_12975"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPG87604.1"
                     /translation="MEGVLDWGVEIIRAIQTVASPGLTAVMRALTLMGSEYFYLIFLP
                     ILFWCVDERYGARFGLVFLLSSFVNGWLKIVFAQPRPYHLDPAVGLGTESSYGLPSGH
                     AQGTATFWGLLAPKIRRPWGLVLALAVPLVISFTRLYLGLHFPTDLFLGLALGWAFAI
                     AGIVLGDRAARLLASWNVRVRILLAAGIALAMNALNMKDTNLSGIFFGTAVGACFLFD
                     RLRFDASSGGFGQKALRLALGLAGVAAIYFGVKLMAPAWGEPLYALFRFVRYALVGAW
                     VSFGAPWAFMRLGLAGARPAEG"
     gene            complement(1041..>1575)
                     /locus_tag="PLQ29_12980"
     CDS             complement(1041..>1575)
                     /locus_tag="PLQ29_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPG87605.1"
                     /translation="GLPDIPVSINLSPRQFGQEFLVDDITAIIESTGIDPRRLEFEIT
                     ESGIMTNERESIRKLLELKALGAAVSIDDFGTGYSSFSKLKDYPVDTIKMDKSFIDPL
                     PHDKKASIIASAIVDMAHTLSFSVVAEGVETLDQMAFLDTIFCDQYQGYLFSKPVPED
                     EFRLMLSAGAALLPRSA"
BASE COUNT          296 a          488 c          539 g          252 t
ORIGIN      
        1 gtagtcggcg tagaccggcg cggcgtggag catgcccgct atcgatatgt cgtcggcgta
       61 ggtggcgcgc ccggttactt tggcgcgcgc gtcgttgcgg aaggcggtat cgggccgcat
      121 cgctcagcct tcggccggcc tggcgcccgc caggccgagg cgcatgaagg cccagggggc
      181 gccgaacgaa acccaggccc cgacgagggc gtagcggacg aagcggaaca gcgcgtagag
      241 cggttcgccc caagccggcg ccatcagctt gacgccgaag tagatggccg cgaccccggc
      301 gaggccgagg gccagccgta gggccttctg gccgaagccg ccggacgagg cgtcgaagcg
      361 caatctatcg aataggaagc aggctccgac ggccgtgccg aagaagatgc cggacaggtt
      421 cgtgtccttc atattgagcg cgttcatcgc gagcgcgatg cccgcggcga ggagtatcct
      481 gacgcgcacg ttccaggatg cgaggagcct ggccgccctg tcgcccagga cgatgccggc
      541 tatcgcgaag gcccagccga gggccaagcc caggaacagg tccgtcggga agtggagccc
      601 gaggtacagc ctcgtgaagc tgatgacgag cggcacggcc agggccagga cgaggcccca
      661 gggcctgcga atcttcggcg ccaggagccc ccagaaggtg gccgtgccct gggcgtgccc
      721 cgaaggcagg ccgtagctcg actcggtgcc caggccgacc gccgggtcga ggtgataggg
      781 cctcggctgg gcgaagacta tctttagcca gccattgacg aagctcgaca acaggaagac
      841 cagcccgaaa cgcgcgccgt agcgctcgtc gacgcaccag aacaggatgg gcaggaagat
      901 caggtagaag tactcggacc ccatcagggt caacgcgcgc atgaccgcgg tcaggcccgg
      961 gctcgctacg gtctggatgg cccggataat ctcgactccc cagtccaaga cgccttccat
     1021 cgttcgctcc tctcttcgat ctacgcgcta cgcggcaaca gggccgcgcc tgccgacagc
     1081 atgagcctga attcatcttc cggaacgggc ttcgagaata ggtagccctg gtactggtcg
     1141 cagaatatcg tgtcgaggaa agccatctgg tccagagtct cgacgccctc ggccacgacg
     1201 ctgaagctca gggtgtgcgc catatcgacg atggccgacg cgatgatcga ggccttcttg
     1261 tcgtgcggca gcgggtcgat gaacgacttg tccatcttga tcgtgtccac ggggtagtcc
     1321 ttgagcttcg agaacgacga gtagccggtg ccgaagtcgt ctatcgagac ggcggcgccg
     1381 agggccttga gctcgagcaa cttccgtatg ctctcacgct cgttggtcat gatgccgctc
     1441 tcggtgatct cgaattcgag ccgccgcgga tcgatgccgg tggattctat tattgccgtt
     1501 atatcgtcga ccaggaactc ctggccgaac tggcgcggcg acaggttgat cgagacgggg
     1561 atgtccggaa ggccg
//
LOCUS       DAOPHV010000648         1567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_115116_length_1567_cov_1.681217, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000648 DAOPHV010000000
VERSION     DAOPHV010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115116_length_1567_cov_1.681217"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="PLQ29_12985"
     CDS             complement(<1..1093)
                     /locus_tag="PLQ29_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87606.1"
                     /translation="MNRLRSSRTAPRALACLALVAVLCAGNAFALDFGLTLSNDSELS
                     GGGGLSLSQSDSAVLWLSLPIGASSSIYASAVYEFSGDFTYLPSYSDRVRWYSITAGR
                     VEWEGFVGLSESMGLGWSVGRIPLQDYSGRVLNGLLDGAVVRLSAGQAIVSAAVAYTG
                     LTFKDDAMILIDADDESAWSDDEVAFAPRRLLASLGLRLVELVPFHDLGLDGWAQFDL
                     AAAGGTATHPQYAEPFIEGRVARSIRWRLWGAAEFGQDPGFFYGMAAGGRIRLSAPEL
                     AGLRVTASCAWAGGDYDGDGAMRAFKPITTGSLSSVAGTMFSDALGASLDASFVPLRG
                     LTIGAGCGANFRPNGDDSYLGFELTGRVEY"
     gene            complement(1090..1542)
                     /locus_tag="PLQ29_12990"
     CDS             complement(1090..1542)
                     /locus_tag="PLQ29_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87607.1"
                     /translation="MKRVCASLILASLCVVAAIGQSNDFVDGLIASDSVSVGQAAYLV
                     LVASDNLGEDADAARAFELLGTFGWAPTGASADVPILLKDYAYLLMSAFGLRGGLMYA
                     LFPGPRYAYRQLVSSFVIQGRSDPDMAVSGPVAVRMLGRVFDVKGIAE"
BASE COUNT          310 a          504 c          541 g          212 t
ORIGIN      
        1 tatattcgac gcgtccggtc agctcgaaac cgagatacga gtcgtcacca ttcggcctga
       61 aattggctcc gcatccggca ccgatagtca agccgcgcaa ggggacgaag gacgcgtcga
      121 gcgacgcgcc gagcgcgtcg gagaacatcg tcccggcgac ggacgatagc gaaccggtcg
      181 tgatcggctt gaaggcccgc atcgccccgt cgccgtcgta gtccccgccg gcccaggcgc
      241 aggaagccgt gacgcgcagc ccggccagct ccggcgccga cagcctgatc cgcccacccg
      301 ctgccatgcc gtagaagaag ccgggatctt gaccgaattc ggccgcgccc cagagccgcc
      361 agcgtatcga ccgcgcgacg cggccctcga tgaagggctc ggcgtattgc ggatgggtag
      421 cggtcccgcc ggcggctgcc aggtcgaact gggcccagcc gtcgaggccg aggtcgtgga
      481 acggtacgag ctcgaccagg cgcaggccga gtgaggcgag caggcggcgc ggggcgaagg
      541 ccacctcgtc gtccgaccat gcgctctcgt cgtcggcgtc tatcaggatc atcgcgtcgt
      601 ccttgaaagt cagcccggta taggccacgg ccgcgctcac gatcgcctgc cctgccgaca
      661 ggcggacgac ggccccgtcc aggaggccat tcaggacgcg gcccgaatag tcctggagcg
      721 gtatacggcc gaccgaccag ccgaggccca tcgactcgct caggccgacg aagccttccc
      781 actcgacgcg tccggcggtg atcgaatacc agcggacgcg gtccgagtac gagggcaggt
      841 aggtgaagtc gcccgagaac tcgtagacgg cggacgcgta gatggacgag gaggcgccta
      901 tcggtagcga cagccataac acggcggagt cgctctgcga cagcgatagg ccgccgccgc
      961 cggacagctc ggagtcgttg gatagggtaa ggccgaaatc gagggcgaag gcgttgccgg
     1021 cgcataagac cgcgacgagg gcgagacagg cgagggccct aggggccgtg cggcttgatc
     1081 tcaggcggtt cattcggcta ttcccttcac gtcgaacacg cggccgagca tcctgacggc
     1141 gaccgggccg gagacggcca tatcagggtc ggaacggccc tggatgacga agctcgagac
     1201 gagctgccta taggcatagc gaggcccggg gaacaacgcg tacatgaggc cgccccgaag
     1261 gccgaaagcg ctcatcaaga gatacgcata gtccttgagt agaatgggca cgtcggcgga
     1321 agcgcccgtc ggagcccagc cgaaggtgcc gaggagctcg aaggcgcggg cggcatcggc
     1381 gtcctcgccg aggttgtccg aggcgaccag cacgaggtag gccgcctggc cgacgctgac
     1441 cgagtccgac gctatcaggc cgtcgacaaa gtcgttcgac tgcccgatgg cggcgacgac
     1501 gcatagcgac gccaggataa gggatgcaca tactcttttc atatgccacc tttaaacggg
     1561 aatatgc
//
LOCUS       DAOPHV010000649         1564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_115358_length_1564_cov_4.807157, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000649 DAOPHV010000000
VERSION     DAOPHV010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115358_length_1564_cov_4.807157"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /gene="ybeY"
                     /locus_tag="PLQ29_12995"
     CDS             <1..273
                     /gene="ybeY"
                     /locus_tag="PLQ29_12995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPG87608.1"
                     /translation="PTDVLSFSLGEWRDGENGRRYIAGDVVICLGVMERNAAEYGATR
                     DEELKRLIAHGVLHLSGMDHGTNEPDEPMLARQEALLSTLAEETIF"
     gene            297..1061
                     /locus_tag="PLQ29_13000"
     CDS             297..1061
                     /locus_tag="PLQ29_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPG87609.1"
                     /translation="MRRAFRSRPDGDEADEVSSERQDMIRGIQELSETTVKEVMVPRI
                     DTVFIAIDAPRDELLETVTTCGHSRIPVYNETIDDVVGVLYVKDLLLAIAKGEEIDLA
                     AMIRKPFFVPESKRIDSLLREFKRRHVHIAIAIDEYGGVSGIVCMEDIIEEIVGDIQD
                     EFDNEREDIVAIGDAAWLCDARVNLEDLNEETGLGLPVEGFDTLGGFVFDLFGKIPAR
                     YEKVSWRTFDFVVQEMDGHKIGAVKILRRPGDVEDA"
     gene            1058..>1564
                     /locus_tag="PLQ29_13005"
     CDS             1058..>1564
                     /locus_tag="PLQ29_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG87610.1"
                     /translation="MTRPARRFGTAVALAAIALVVAGAPPVSAQAAKPVSARERYEAG
                     VELLASGATYRAVDAFMAAVAANPAYADAWAMLARCQYELGEYERAVSFIAEAYKYGP
                     RSPSLVTLEAFSRIGQGKLAEARALLDEALARLPNDRDARFGLALLDLRGGRPGDARA
                     RLASSLKTA"
BASE COUNT          263 a          516 c          536 g          249 t
ORIGIN      
        1 ccgaccgacg tgctgtcgtt ctcgctcggc gagtggcgcg acggcgaaaa cggccgtcgc
       61 tatatcgccg gagatgtggt aatatgtctg ggcgtcatgg agcgtaacgc cgccgagtac
      121 ggcgccacgc gcgacgagga gcttaagcgc ctcatcgcgc acggtgtcct gcacctgtcc
      181 ggcatggacc acggcacgaa cgagcccgac gagccgatgc tcgccaggca ggaggcgctc
      241 ctatcgacgt tagcggagga aacgatattt tgaagacgag cggtttcttg agcggcttga
      301 ggagggcctt ccgctccagg cccgacggcg acgaggccga cgaggtgagt tcggagcgcc
      361 aggacatgat acgcggcatc caggagctgt ccgagaccac ggtcaaggaa gtgatggtgc
      421 ccaggatcga cacggtgttc atcgccatcg acgcgccgcg cgacgagctt ctcgagacgg
      481 tgaccacctg cgggcactcg aggatacccg tctacaacga gaccatcgac gacgtcgtcg
      541 gggtgctgta cgtcaaggac ctgctgctcg ccattgccaa gggcgaggag atcgacctgg
      601 ccgcgatgat acgcaagccc ttcttcgtgc ccgagtcgaa gcgcatcgac tccctgctcc
      661 gggaattcaa gcgccggcac gtgcatatag ccatagccat cgacgagtat ggcggcgtgt
      721 cgggcatcgt ctgcatggag gatatcatcg aggagatcgt cggcgatatc caggacgagt
      781 tcgataacga gcgggaggat atcgtcgcca taggcgacgc ggcctggctg tgcgacgcgc
      841 gcgtcaatct cgaggatctg aacgaggaga ccgggctcgg cctgcccgtc gagggtttcg
      901 atacgctcgg cggcttcgtc ttcgacctgt tcggcaagat tcccgcccgc tacgagaagg
      961 tgtcctggcg cacgttcgac ttcgtcgtcc aggagatgga cggccataag atcggcgccg
     1021 tcaagatact caggcggccg ggcgacgtag aggacgcatg acgcggccgg cgcggcgctt
     1081 cggaaccgcg gtggcgctcg ccgccatcgc gctggtcgtc gcgggcgctc ccccggtctc
     1141 cgcccaggcg gccaagcccg tctcggcgcg cgagcgctac gaggccggcg tggagttgct
     1201 ggcgtccggc gctacctacc gcgccgtcga cgcgttcatg gcggccgtcg ccgccaaccc
     1261 cgcctacgcc gacgcgtggg ccatgctggc ccgttgccaa tacgagctgg gcgagtacga
     1321 gcgcgcggta tccttcatcg ccgaggctta caagtacggg ccgcgctcgc cgtccctggt
     1381 caccctggag gcgttctcgc gcatcgggca gggcaagctg gccgaggccc gcgccctgct
     1441 cgacgaggcc ctcgcccggt tgccgaacga ccgcgacgcc cgcttcggcc tggccctgct
     1501 cgacctgagg ggcggccggc ccggcgacgc cagggccagg ctcgcctcgt cgctcaagac
     1561 cgcc
//
LOCUS       DAOPHV010000650         1564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_115376_length_1564_cov_3.487740, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000650 DAOPHV010000000
VERSION     DAOPHV010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115376_length_1564_cov_3.487740"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PLQ29_13010"
     CDS             complement(<1..230)
                     /locus_tag="PLQ29_13010"
                     /inference="COORDINATES: protein motif:HMM:NF015442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HPG87611.1"
                     /translation="MAETQQVFPEWRSFLKAEDGQGRYVAKRSGSVERYDRSRIESAI
                     EKAFKAVKGVADKDLVQRIAADVESRLALMLSG"
     gene            complement(232..702)
                     /gene="nrdR"
                     /locus_tag="PLQ29_13015"
     CDS             complement(232..702)
                     /gene="nrdR"
                     /locus_tag="PLQ29_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPG87612.1"
                     /translation="MRCPHCGSMDDRVTDSRTLANGESIRRRRECLSCGFRFTSYERI
                     EEKPLMVVKRDGRREPFDRQKMERGLQRALEKRPVSQMTIENILNEIEDDAAMLGKGG
                     NEVQSSALGEMTLHKLYDLDKVAYIRFASVYRKFETMDEFMQEIERLENAREGE"
     gene            939..1280
                     /locus_tag="PLQ29_13020"
     CDS             939..1280
                     /locus_tag="PLQ29_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87613.1"
                     /translation="MTFSERMKQLVDQGISASKELARQAGDKAQEWGEKGVLKLEVVQ
                     LRNQAEKLTARLGAEVYEVLVEKGQKSVGKDSPAVRETIDNIEALRAQIEVKEQAFKK
                     AGGKEEDLASD"
     gene            complement(1272..>1564)
                     /locus_tag="PLQ29_13025"
     CDS             complement(1272..>1564)
                     /locus_tag="PLQ29_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA methyltransferase"
                     /protein_id="HPG87614.1"
                     /translation="PARVKFLARRQWSLLSSAFLLLAPGGCLVYATCALSPAENDGPM
                     RRLIEKYEGLAELDPPEAEAGAERTEYGIHYLPDVASGAGPLYVARIRKRSV"
BASE COUNT          306 a          480 c          476 g          302 t
ORIGIN      
        1 cccgagagca tcaacgcaag tcgcgactcg acgtcggcgg cgatacgctg gaccaggtcc
       61 ttgtcggcga cgcccttgac ggccttgaac gccttctcga tggcggactc gatgcggcta
      121 cgatcgtagc gctcgacgct accggagcgc ttggcgacgt agcgcccctg tccgtcctcg
      181 gccttcagga agctgcgcca ctcggggaag acctgttgcg tctcagccat ctcactctcc
      241 ctcgcgagcg ttctctagcc gctcgatctc ttgcatgaac tcgtccatgg tctcgaattt
      301 cctatacacg ctggcgaaac ggatgtacgc gaccttatcc aggtcgtaga gcttgtgcag
      361 ggtcatctcg ccgagcgccg agctctggac ctcgttgccg cccttgccga gcatggccgc
      421 gtcgtcctct atctcgttga ggatgttctc aatggtcatc tgggagaccg ggcgcttctc
      481 gagggcgcgc tggagtcctc gttccatttt ctgccggtcg aagggctcac gcctgccgtc
      541 gcgcttgacc accatcagcg gcttttcctc gatgcgctcg tagctcgtga agcggaagcc
      601 gcaggacagg cactcgcggc gccgcctgat gctctctccg ttggcgagcg tgcgtgagtc
      661 cgtgacccga tcatccatcg atccgcagtg agggcacctc atttttttta aacctccaca
      721 gaatcgaaca acgggaacaa cggcggcgca cgacttggta cgattcttgc tattgtacat
      781 ataccagtta tagtatccaa ataccaaacg acagctagat atagtgccta gccgagccct
      841 tgtcaagata gcacgatacc gtggatacgt cattgatttt ttactgtttc cgggcgattt
      901 tagttgagag catcatcatg aacgacaagg agcattgaat gacgttttcc gagcgcatga
      961 agcaactcgt cgaccaggga atcagcgcgt cgaaggagct ggccaggcag gccggcgaca
     1021 aggcccagga atggggcgag aaaggcgtgc tcaagctcga ggtggtccag ctgcgcaacc
     1081 aggccgagaa actgaccgcc aggctcggcg ccgaggtcta cgaggtactc gtcgagaagg
     1141 gccagaagag cgtcggcaag gattccccgg ccgtccgcga gaccatagac aacatcgagg
     1201 ccctgcgcgc ccagatcgaa gtgaaggaac aggccttcaa gaaggccggc ggcaaggaag
     1261 aggacctcgc gtcagactga gcgcttgcgt atcctggcca cgtagagcgg tccggctccc
     1321 gaggcgacgt cgggaaggta gtggatcccg tactcggtcc gctcggcgcc tgcctcggct
     1381 tccggtggat cgagttcggc caggccttcg tacttctcga tcaggcgacg catcgggcca
     1441 tcgttctccg cgggcgacag ggcgcaggtc gcgtagacca ggcatccgcc cggcgccaac
     1501 aggaggaagg cgctcgacaa gagggaccat tgcctcctgg ccaggaactt gacgcgcgcc
     1561 gggc
//
LOCUS       DAOPHV010000651         1563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_115463_length_1563_cov_4.026525, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000651 DAOPHV010000000
VERSION     DAOPHV010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115463_length_1563_cov_4.026525"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PLQ29_13030"
     CDS             complement(<1..1099)
                     /locus_tag="PLQ29_13030"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HPG87615.1"
                     /translation="MSRSPVRFFKSVSQRVVFSLEQFSRHEMANHAAAGAYAFLLSAI
                     PAVLVILFVSSRVLGTLDSGLVLGVLDPIIAVFGGREAIASFVSTPLAGFTGAFGVVN
                     LLWAARLFIVSMQRGMRIVYADGAKVNPVRENALTFAVEIILILAIVLIISASQVARA
                     AIGAIHWKPMARLLGLAVSLGLHAFPTLALWLFVFLTYKNVPPVKPRAVNAAIGSAAC
                     LVTYTALGALLSLTLNTARYGLLYGILGNLIIGLIKVYFFFWLYFFFAELCHTLENFD
                     SMLFARFHRLAMSDKPAKGLDRTLFSNPARLIRRYAREYSAGETIFEKGDDDRSALYL
                     YEGSVEIGLPPAAGEPGAILSTVGEGELFGEM"
     gene            complement(1155..>1563)
                     /locus_tag="PLQ29_13035"
     CDS             complement(1155..>1563)
                     /locus_tag="PLQ29_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87616.1"
                     /translation="KPGAITAQGFLGDDERALADIIEADLEAWRRAGVDPDDAATFLE
                     RLESLGQKGLGEPTVVDGRWTVTVGDARGRLPCPFHDGAFHKNSIEAVDGTNGARVVY
                     SDLSIHLLRRHRFCQGLGSPFRLDPEVVASMMR"
BASE COUNT          287 a          497 c          542 g          237 t
ORIGIN      
        1 ccatctcgcc gaagagctcg ccctcgccga ccgtcgacag gatggcgccc ggctcgccgg
       61 cggccggggg cagccctatc tccaccgagc cctcgtacag gtagagcgcg ctacggtcgt
      121 cgtcgccctt ctcgaagatc gtctcgccgg cgctgtattc ccgggcatag cgcctgatca
      181 gcctggccgg gttggagaac agcgtgcggt cgaggccttt ggccggcttg tccgacatcg
      241 ccagccggtg gaagcgggcg aacagcatgg agtcgaaatt ctcgagagtg tggcatagct
      301 cggcgaagaa gaagtagagc caaaagaaga agtatacctt gatgagcccg atgatcaggt
      361 tcccgaggat accgtacagg aggccatagc gcgccgtgtt cagggtcagg ctgagcaggg
      421 ccccgagcgc cgtataggtt accaggcagg ccgccgatcc gatcgcggcg ttgacggctc
      481 gcggcttgac cggcggcacg ttcttgtacg tcaggaagac gaacagccag agggccagcg
      541 tcgggaacgc atggagcccc aggctgaccg ccaggcccaa gagtcgcgcc atcggtttcc
      601 agtggatggc gccgatagcg gccctggcca cctggctggc cgagatgatg agcacgatcg
      661 ccaggatgag gatgatctcg acggcgaagg tcagggcgtt ctcgcggacc gggttgacct
      721 tggctccgtc ggcgtagacg atgcgcatgc ctcgctgcat cgacacgatg aacagtctgg
      781 ctgcccagag caggttgacg acgccgaacg ccccggtgaa accggccagg ggcgttgaaa
      841 cgaagctcgc tatcgcttca cggccgccga atacggcgat gatcgggtcg agcacgccga
      901 gcaccaggcc cgagtcgagc gtgccgagca ctcgggacga gacgaacagg atgacgagga
      961 ccgcggggat cgccgacagc aggaacgcgt aggcgcccgc cgcggcgtgg ttggccatct
     1021 catggcgcga gaactgttcc aatgagaaga cgacgcgctg cgagacggac ttgaagaatc
     1081 ggaccgggga cctgctcatc gcgtcattgt gcaacggccg ggagcgatcg cgcaagggcg
     1141 cggtgccacg gcggctatcg catcatcgag gcgactacct cggggtccag cctgaacgga
     1201 ctacccaggc cctggcagaa cctgtgccgc ctcaggaggt ggatcgacag gtccgagtat
     1261 acgacgcgcg cgccgttcgt cccgtcgacc gcctctatgg agttcttgtg gaaggcgccg
     1321 tcgtggaagg ggcagggtag ccgaccgcgc gcgtccccca cagttacggt ccagcggccg
     1381 tcgacgacgg tcggttcgcc gagtcccttc tggccgagcg actcgagccg ctccaggaac
     1441 gtggcggcgt cgtccgggtc cacgccggcg cggcgccacg cctccaggtc cgcctcgatt
     1501 atatccgcga gcgcgcgctc gtcgtcgccc aggaaaccct gggccgtgat ggcgccaggc
     1561 ttc
//
LOCUS       DAOPHV010000652         1562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_115620_length_1562_cov_2.927007, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000652 DAOPHV010000000
VERSION     DAOPHV010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115620_length_1562_cov_2.927007"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLQ29_13040"
     CDS             <1..426
                     /locus_tag="PLQ29_13040"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPG87617.1"
                     /translation="LLFNMLGLFFFGAAVERSLGSREFLFFYLLTGALAGLFSLAAFA
                     LGGAMGVSLVGASGAVYAVLLAFAVINPRSRIFIWGVVPVPAPILVAGYTVIELWSQL
                     FGSGGGVAHLTHLAGFAFAALYFPIRHGVNPIKRLASGR"
     gene            434..1168
                     /locus_tag="PLQ29_13045"
     CDS             434..1168
                     /locus_tag="PLQ29_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87618.1"
                     /translation="MRTTIGRSGTPPSRAARRFAVAVVALLSAASSVAGQDRDVILRA
                     EFPVDLASPPSVLAFRDVADPGYVARVPDGEAASALLAEARWTFSGMIWGFSYSYTPS
                     DRARSIDERFELAPLAPEASAAMMPRAVAARLDGTVLYATVEFYPDASQRAELASWKA
                     SSAAAQGRGRAPFLAPGARRLAVEDAVREALRAYLRQVTHNKPREVRGTVALAAPPRL
                     SLLEGSWVASARVLARVDEIVSYGAY"
     gene            complement(1169..>1562)
                     /locus_tag="PLQ29_13050"
     CDS             complement(1169..>1562)
                     /locus_tag="PLQ29_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPG87619.1"
                     /translation="AINLASMKKLAELRTQGKLVLVFPAGTRFRPWDPASKRGVREID
                     SYIRSFDYMSLVSINGNILRINPEGEMLEDLICRDAVRYGVSEPRRCDEFRDAVKASL
                     AEGQDRKQAIVDAVMAELDAMHLAAERA"
BASE COUNT          184 a          562 c          540 g          276 t
ORIGIN      
        1 cttctcttta acatgctcgg cctgttcttt ttcggcgccg cggtcgagcg ctcgctcggc
       61 tcgcgggagt tcctgttctt ctacctgctg accggcgccc tggcggggct gttctcgctc
      121 gccgccttcg ccctgggcgg cgccatgggc gtcagcctcg tcggcgcgtc cggcgccgtc
      181 tacgcggtgc tgctagcgtt cgcggtgatc aacccgcggt ccagaatctt catctggggc
      241 gtggttccgg tacccgcccc gatcctcgtg gccggctaca ccgtcatcga gctgtggtcg
      301 cagctgttcg gctcgggcgg cggcgtcgcc cacctgaccc acctggccgg cttcgcgttc
      361 gccgcgctgt attttccgat ccggcatggc gtcaacccga tcaagcgcct cgcctcggga
      421 cgctgaggca tccgtgagga cgacgatagg ccggtccgga acgccgccct cgcgggcggc
      481 gcgccgcttc gcggtcgccg tcgtcgccct gctatcggcc gcctcgagcg tcgccggcca
      541 ggaccgcgac gtcatcctgc gggccgaatt cccggtcgac ctggcgtctc cgccgtcggt
      601 gctggccttc cgggacgtgg ccgaccccgg ctacgtagcc agggtgcccg acggcgaggc
      661 ggcctcggcc ctgctcgccg aggcgcgctg gaccttctca gggatgatat ggggcttctc
      721 gtactcgtat acgccgagcg accgcgcgcg ctcgatagac gagcgcttcg agctggcgcc
      781 gctggctccg gaggcgtccg ccgcgatgat gccgagggcg gtcgccgcca ggctcgacgg
      841 taccgtgctg tacgcgacgg tcgaattcta cccggacgcg tcgcagcgcg ccgagctggc
      901 ttcgtggaag gcgtcgagcg cggccgccca gggcagggga cgggcgccgt tcctcgcgcc
      961 gggcgctagg cgccttgccg tcgaggacgc cgtccgcgag gcgctcaggg cctacctgcg
     1021 ccaggtaacg cacaacaagc cgcgcgaggt gcgcggcacg gtggcgctgg ccgcgccgcc
     1081 gcggctgtcg cttctcgagg gtagctgggt cgcgagcgcg cgggtactgg cccgcgtcga
     1141 cgagatcgtc tcgtacggcg cgtactagct acgcgcgctc ggccgccaag tgcatcgcgt
     1201 cgagctccgc catcaccgcg tcgacgatgg cctgcttgcg gtcctgtccc tcggcgaggg
     1261 acgccttcac cgcgtcgcgg aactcgtcgc agcgcctcgg ctcgcttact ccgtagcgga
     1321 ccgcgtcgcg gcagatcagg tcctcgagca tctccccctc cgggttgatg cgcaggatgt
     1381 tgccgttgat cgatacgagc gacatatagt cgaacgagcg gatgtacgag tctatctccc
     1441 gcacgccgcg ctttgaggcc gggtcccagg gcctgaaacg ggtgcccgcg gggaagacca
     1501 gcaccagctt gccctgggtc cgcagctccg ccagcttctt catcgaggcc aggttgatcg
     1561 cg
//
LOCUS       DAOPHV010000653         1558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_116033_length_1558_cov_4.284764, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000653 DAOPHV010000000
VERSION     DAOPHV010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116033_length_1558_cov_4.284764"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PLQ29_13055"
     CDS             <1..419
                     /locus_tag="PLQ29_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPG87620.1"
                     /translation="GLTFALRNGPLVAISGIITGIAAAFSMAASDYLASKADGDPRAA
                     KSALYTGVAYLVTVVLLVMPYLVLPRDGSWIFVSLAITLAIAVAIIAAFNFYLAVAKD
                     LNFKRRFGEMAGISLGVSAFSFGVGLVVRAVFGIDV"
     gene            519..1343
                     /locus_tag="PLQ29_13060"
     CDS             519..1343
                     /locus_tag="PLQ29_13060"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPG87621.1"
                     /translation="MDARTQVREAAGYIAARIRVKPTIALVLGSGLGSLADEVEDPVA
                     LPYGEIPHFPSSTAPGHAGRLVVGTMAGKAVLVMQGRFHYYEGHPLERIAFPVRVFKA
                     LGIDTLFLTNAAGGANEGYKPGDFMIIRDHINIMGQNPLIGPNDDELGPRFPDMSTTW
                     TPELADAAKRAAVDVGVAAREGVYMWFTGPSFETPAEVRLARMLGADAVGMSTVPEAT
                     VARHCGMRVVGISCITNMAAGILPQPITGEEVIEIANQRRPQFVALVKRTLELIQG"
BASE COUNT          270 a          557 c          467 g          264 t
ORIGIN      
        1 caggcctgac cttcgcgctg cgtaacggcc cgctcgtagc gatatcgggc atcatcacag
       61 gcatagcggc ggccttctcc atggcggcct ccgactacct ggccagcaag gccgacggcg
      121 acccgagggc ggccaagtcc gccctgtaca ccggagtcgc ctacctcgtc acggtcgtcc
      181 tcctcgtcat gccctacctc gtgctacctc gggacggatc ctggatcttc gtcagcctgg
      241 cgatcacgct cgccatcgcc gtcgccatca tcgcggcgtt caacttctac ctagccgtcg
      301 ccaaggacct gaatttcaag cgccgcttcg gcgagatggc gggcatcagc ctgggcgtct
      361 cggcgttctc gttcggcgtc ggcctcgtcg tcagggccgt gttcggcatc gacgtataag
      421 gccgcccgag gccacggcac cgaggcccgg ccgccattga caggccatgg gcccgctggt
      481 agcctgtggc cccaccttag ccactcgagg agagcgacat ggacgctaga acacaggtac
      541 gagaagccgc cggttatata gccgcgagga tacgggttaa gccgacgata gccctggtgc
      601 tcggctcggg cctcggcagc ctggccgacg aggtcgaaga cccggtcgcc ctgccctacg
      661 gcgagatccc gcacttcccc tcgtcaaccg cccccggcca cgcgggccgc ctcgtcgtcg
      721 gcacgatggc cggcaaggcc gtgctcgtga tgcagggccg cttccattac tatgagggcc
      781 atccgctcga gcgcatagcc tttccggtgc gcgtcttcaa ggcgctcggc atagacacgc
      841 tcttcctgac caatgcggcc ggcggcgcta acgaaggcta taagcccggc gacttcatga
      901 tcatccgcga ccacatcaac atcatgggcc agaacccgct cataggaccg aacgacgacg
      961 aactcggtcc gcgcttcccc gatatgtcga cgacctggac gcccgagctg gcagacgccg
     1021 ccaagcgggc ggccgtcgac gtaggcgtag ccgcgcgcga gggcgtctac atgtggttca
     1081 ccgggccgag cttcgagacg ccggccgagg tcaggctcgc gcgcatgctc ggcgccgacg
     1141 ccgtcggcat gtccaccgtg cccgaggcca ccgtcgcccg ccactgcggc atgcgcgtcg
     1201 tcggcatatc gtgcatcacc aacatggccg ccggcatcct gccccagccc atcaccggcg
     1261 aggaggtcat cgagatcgcc aaccagcgcc gaccccagtt cgtcgccctg gtcaaacgga
     1321 cattggagct gatccaaggc taggcccgcc cggtcggttt tctattgaca tatcgcctat
     1381 acggggtaca ctgcatacaa agtgagtgat tgctcacttt atgctatgac gcgcgcctag
     1441 cggcgcgccg ccctaaggag agccatatgt cattcgctat cagctcgtac gacaagcccg
     1501 acgagatata ttccgccctg gacgccctcg tctcgcggcc gccggtgccg ctcaagaa
//
LOCUS       DAOPHV010000654         1555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_116369_length_1555_cov_4.934667, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000654 DAOPHV010000000
VERSION     DAOPHV010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116369_length_1555_cov_4.934667"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1555)
                     /locus_tag="PLQ29_13065"
     CDS             complement(<1..>1555)
                     /locus_tag="PLQ29_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87622.1"
                     /translation="VRRVEKGQSVTVPLYAVFDERVLALTENGSTSAEIAVEYSYVGT
                     RRTAKEGLSLSLHHRNAMSWEDDRRAAAFVSPTDPAVLWFSRYSASVVRDRLRTELPP
                     NLQYALGLFEALKLYGLNYVIDPNSAYVDMSESAAVVDYLQYPSQTLMYRGGDCDDLS
                     ILFCSLLESQGIATAFITIPGHIYMAFDLGLSEAEARERFFDPGLLLFRDGRAWAPVE
                     ITMVKDGFVKAWRVGAKQWIDNDRVGTAAFYAMRDAWIEYKPSGLQDAATRFSLPDEA
                     RAMAAFDSGVDRFVAREMEPVVAEYERKLARRREPEVLNEYGTAMARVGMLDQAWERF
                     AEASKAGYAWAWNNLASIAFVRKDYELAYSYYEWTTTLLPGDPVAVLGMARCAYELDR
                     YAEAAILYEALTVDAPALAVKYGYLASAYGGTGRAWSMADRLAGSVWSRPDMSFDLPS
                     SAVAPAVARTAPVAPTPAPAARIAPPPADDTPAETEKVAVPTPTASPEPAVEPEPAAK
                     AEPAVEPEPA"
BASE COUNT          234 a          510 c          562 g          249 t
ORIGIN      
        1 gccggctcgg gctcgaccgc tggctcggcc ttggctgccg gctcgggctc gaccgcgggt
       61 tcggggctcg cagttggcgt aggcacggcg accttctcgg tctcggcagg cgtgtcgtcg
      121 gccggcggcg gcgcgatccg ggctgccggg gcgggcgtcg gggctaccgg cgcggtcctg
      181 gccaccgccg gcgccacggc gctcgagggc aggtcgaagc tcatgtcggg cctcgaccag
      241 acggagccgg cgaggcggtc ggccatggac caggcgcgcc cggtcccgcc gtaggccgag
      301 gccaggtagc cgtacttgac agccagcgcc ggggcatcga cggtgagggc ctcgtagagt
      361 atggccgcct cggcgtagcg gtccagctcg taggcgcagc gcgccatgcc gagcaccgcg
      421 acggggtcgc cgggcagcag ggtcgtcgtc cattcgtagt acgaataagc cagctcgtag
      481 tctttgcgta cgaaggcgat gctggccagg ttgttccagg cccaggcgta gccggccttg
      541 gaggcctcgg cgaaacgctc ccaggcttgg tcgagcatgc cgacgcgggc catcgccgtg
      601 ccgtactcgt tgagtacctc gggctcgcgt cgcctggcca gcttcctctc gtactcggcg
      661 acgaccggct ccatctcgcg ggccacgaag cggtcgacac ccgagtcgaa ggcggccatc
      721 gcgcgggcct cgtcgggcaa cgagaagcgg gtcgcggcgt cttgaaggcc gctcggctta
      781 tactcgatcc aggcgtcgcg catcgcatag aaggccgcgg taccgacccg atcgttgtct
      841 atccattgct tggcgccgac acgccacgcc ttgacgaagc cgtccttgac catcgttatc
      901 tcgaccggcg cccaggcgcg gccgtccctg aacagaagca gaccggggtc gaagaaacgc
      961 tccctggcct cggcctcgga gaggcccagg tcgaaggcca tgtagatatg gcccgggatc
     1021 gtgatgaagg ccgtcgctat gccctgcgac tcgagcaggg agcagaacag gatggacagg
     1081 tcgtcgcagt cgcctcctcg gtacatcaag gtctggctcg gatactgcag gtagtcgacg
     1141 acggcggcgc tctcggacat gtcgacgtag gcgctgttcg ggtcgatgac gtagttaagc
     1201 ccgtagagct tgagcgcctc gaagaggccg agcgcgtact gcaggttcgg cggcagctcg
     1261 gtgcgcagtc ggtcgcgcac gacgctggcc gagtagcggg agaaccagag caccgccggg
     1321 tcggtcggcg agacgaaggc ggcggcgcgc cggtcgtcct cccaggacat cgcgttgcgg
     1381 tggtggagcg acaggctcag gccttccttg gccgttcgcc gcgtgccgac gtacgagtac
     1441 tcgaccgcta tctcggcgct cgtcgagccg ttttccgtga gggccagcac ccgctcgtcg
     1501 aagacggcgt acaggggcac ggtaacgctc tggcctttct cgacacgcct gacga
//
LOCUS       DAOPHV010000655         1555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_116375_length_1555_cov_4.288667, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000655 DAOPHV010000000
VERSION     DAOPHV010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116375_length_1555_cov_4.288667"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1509)
                     /locus_tag="PLQ29_13070"
     CDS             complement(<1..1509)
                     /locus_tag="PLQ29_13070"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HPG87623.1"
                     /translation="MIPDGALVFYKNKPAVAHRDGDRIELSFKEGAPVRVRDKDVELI
                     HTGPVKAPPEPAVGGEFEPAWEMTAGTNLGLAELAELAFGSAGPAEVLACRLAAAEGL
                     PFRMDGELIASMSTEDRSAEESRRKRKETEASERAAFVERARHHAVEPGDERFWGELE
                     ALALGRTQKSRTAGDIGVGENPESAQAWLIKAGIWTNRVNPHPSRSGHPSKAPELELG
                     PDDDESRVDLRSMEAWAIDNAWSHDPDDAISWDGEAVWVHVADPASVIVPGSAPDVEA
                     SNRSGTLYLPEGAIPMLPDAALDRFGLGLTNESRALSFRITMDDDGLARDVTVTPSLV
                     RVRRASYAEADVLLGSGALASLAKAAELRKAYRLRNGAVDIAIPEVRVWVEDGEPRIS
                     PVCQYASAAVVREMMVLAGEGLARWAFDRGLPFPYYSQEAPGSRDDLPDGLAGEFAKR
                     RLMKAGMAGVQPRAHQGLGVTMYAQATSPLRRYGDLLGHQQARAALAAEAGRT"
BASE COUNT          232 a          526 c          528 g          269 t
ORIGIN      
        1 ggttctgccg gcctcggcgg ccagggcggc cctagcctgt tggtggccga gcaagtcgcc
       61 gtagcggcgt agtgggctcg tggcctgagc gtacatcgtc acgccgagcc cctggtgggc
      121 tcgaggctgg acgccggcca tgcccgcttt catgaggcgg cgcttggcga actcaccggc
      181 gaggccgtcg ggcaggtcgt cgcgtgagcc cggcgcctcc tggctgtagt acgggaatgg
      241 cagcccgcgg tcgaaggccc agcgggccag gccctcgccg gcgagcacca tcatctcgcg
      301 cacgacggcg gccgatgcgt actggcacac gggcgatatt ctcggctcgc catcctcgac
      361 ccagacgcga acctccggga tggcgatgtc cacggcgccg ttacggagcc tataggcctt
      421 gcgcagctcg gccgccttcg cgagggacgc gagcgcgccg gagcccaaca ggacatcggc
      481 ctcggcgtac gaagcccgcc tgacccgcac gagactcggc gtcaccgtta catcgcgcgc
      541 gaggccgtcg tcgtccatcg ttatcctgaa cgacagggcc cgcgactcgt tcgtgagacc
      601 gaggccgaag cggtcgagcg ccgcgtcggg tagcatcggg atggcgcctt cgggcaagta
      661 cagagtgccg gagcggttgg acgcctccac gtcgggggcg ctgcctggga cgatgacgct
      721 cgcgggatcg gccacgtgta cccagacggc ctctccgtcc caggatatcg cgtcgtcggg
      781 atcgtggctc caggcattgt cgatggccca agcttccatc gaccgcaggt cgactcggct
      841 ctcgtcgtcg tcagggccca gctccagctc gggcgccttg gacgggtgcc cggagcgcga
      901 cggatggggg ttcacgcggt tggtccagat accggctttg atcagccagg cctgagccga
      961 ttctggattt tcaccgactc cgatgtcgcc cgccgttcgc gatttctgcg tacggccgag
     1021 cgcgagggcc tcgagctcgc cccagaaacg ctcgtccccc ggctccacgg catgatgcct
     1081 ggcccgctcg acgaacgcgg ctcgctcgga cgcctcggtc tccttgcgct tacggcggga
     1141 ctcctcggct gatcgatctt cggtggacat cgaggcgatc agctcgccgt ccatcctgaa
     1201 cggtaacccc tcggccgccg cgaggcggca ggccaggacc tcggctgggc ccgccgagcc
     1261 gaaggccagc tcggccagct cggctaggcc aaggttcgta ccagcggtca tctcccatgc
     1321 cggctcgaat tcgccaccga ccgctggctc aggcggcgcc ttgactgggc cggtgtgtat
     1381 aagctcgacg tccttgtcgc gaaccctgac cggagcgcct tctttaaacg acagttcgat
     1441 acggtctcca tcgcggtggg cgactgcggg tttattctta tagaaaacga gggctccgtc
     1501 gggtatcatg gcggtccttt cggggatata aagcaggtga tcagtatacg ggcgt
//
LOCUS       DAOPHV010000656         1553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_116629_length_1553_cov_3.608144, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000656 DAOPHV010000000
VERSION     DAOPHV010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116629_length_1553_cov_3.608144"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLQ29_13075"
     CDS             complement(<1..531)
                     /locus_tag="PLQ29_13075"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPG87624.1"
                     /translation="MELSPLQEARYDYAPKLPSVLSGGVGRVVVEFGEPTESVADRDQ
                     LRALFPACYGKPVARFVQGEGAARRTRMTIGAILSGGQAPGGHNVIAGLFDGLKAGNP
                     ESTLLGFKGGPGGLIDDKAVVLDAATIDRYRNTGGFDIIGSGRTKIETPEQFSKSAAT
                     ARARGLDAIVVIGGDDS"
     gene            complement(650..>1553)
                     /locus_tag="PLQ29_13080"
     CDS             complement(650..>1553)
                     /locus_tag="PLQ29_13080"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPG87625.1"
                     /translation="NLDRVSTFYDQFLKYVDSVYTRYVTKGEISLKELSDKVKTLCDV
                     ILENKRYILRVLSRESGSTNYLVNHSTRSTILAIVLGGHLKLPPHRLIELGVAAVLHE
                     IGMVRLPPQMYMADRKLSDNERKSITAHPILGYNILKDKQFPLSICLAALEHHERMNG
                     TGYPRALTGDKISLYARIIAVACSYDAVTASRPYKEAREGYAGMVDILKNEGKQYDDA
                     VIKSLVYSLSVYPVGSYVLLTNARLGQVIDVNPDNPRYPIVLVLGSKTPDGKETMVQT
                     SETGVRILKPVRREDVERLSQAKG"
BASE COUNT          239 a          470 c          549 g          295 t
ORIGIN      
        1 ggagtcgtcg ccgcctatga cgacgatcgc gtcgaggccc ctggcacggg ccgtagcggc
       61 gctcttggag aactgctcgg gcgtctcgat cttggtgcgg cccgagccga tgatgtcgaa
      121 gccgccggta ttgcggtaac ggtcgatcgt cgcggcgtcc aggaccacgg ccttgtcgtc
      181 gatcaggccg cccgggccgc ccttgaagcc gagcagcgtc gactcggggt tgccggcctt
      241 gagcccgtcg aagagcccgg caatgacgtt gtggccgccc ggggcctggc cgccggagag
      301 gatggcccct atcgtcatcc tggtccgcct cgcggcgccc tcgccctgga cgaacctggc
      361 cacgggctta ccgtaacagg cggggaacaa cgcgcgcagc tgatcgcgat cggcgaccga
      421 ctcggtcggc tcgccgaatt caacgacgac gcggccgacg ccacccgata gtacggacgg
      481 tagcttgggg gcgtagtcgt atctggcttc ctgaagcggg gatagttcca tgattggctc
      541 cttcgtggtc gagccgctcg gcgcggagag ctctggcttc cggaggcggc gttaccatcg
      601 aggataccgg atagagcctc ggggggcaag gtcgagccga cccgaggcct tatcccttgg
      661 cctgggagag gcgctcgacg tcctccctgc gtaccggctt caggatgcga acgccggtct
      721 cggaggtctg gaccatcgtc tccttgccgt cgggggtctt ggagccgagg accaggacga
      781 tggggtatcg cgggttgtcg ggattcacgt cgatgacctg gccgagcctc gcgttggtca
      841 ggagcacgta ggagccgacc gggtagaccg acagcgagta gacgagcgac ttgatcaccg
      901 cgtcgtcgta ctgcttgccc tcgttcttga gtatgtcgac catgccggcg tagccctcgc
      961 gcgcctcctt gtagggccgg gaggcggtta ccgcgtcgta cgagcaggcg accgcgatga
     1021 tgcgcgcgta cagcgatatc ttgtcgccgg tcagcgcgcg cggatagccg gtgccgttca
     1081 tgcgctcgtg gtgctcgagc gccgcgaggc atatcgacag cgggaactgc ttgtccttga
     1141 gtatgttgta gccgagtatc gggtgcgcgg tgatcgactt gcgctcgttg tcggacagct
     1201 tgcggtcggc catgtacatc tgcggcggca gcctgaccat gcctatctcg tggaggaccg
     1261 ccgcgacgcc tagctcgatc agccggtgcg gcggcagctt gaggtggccg ccgagcacga
     1321 tggccaggat ggtcgagcgc gtcgagtggt tgacgaggta gttggtcgag ccggactcgc
     1381 ggctcagcac gcgcaggatg tagcgcttgt tctcgagtat aacgtcgcag agcgtcttga
     1441 ccttgtccga cagctccttg aggctgatct cgcccttggt cacatagcgc gtgtagaccg
     1501 agtcgacgta tttcaggaac tggtcgtaga acgtcgagac gcggtcgagg ttc
//
LOCUS       DAOPHV010000657         1549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_117081_length_1549_cov_3.514726, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000657 DAOPHV010000000
VERSION     DAOPHV010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117081_length_1549_cov_3.514726"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..507
                     /locus_tag="PLQ29_13085"
     CDS             112..507
                     /locus_tag="PLQ29_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HPG87626.1"
                     /translation="MQIEVASIRIRKRIRKDLRDIDQLAASMDRFGQLHPITVTRRNV
                     LIAGRRRLEAARRLGWSTIEATVVGKSDAAQRLELEIEENIQRFPLDRDEIEQALLRL
                     ERLRNPGFLRRAWYAILSFFRRLFGIDDR"
     gene            539..1516
                     /locus_tag="PLQ29_13090"
     CDS             539..1516
                     /locus_tag="PLQ29_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87627.1"
                     /translation="MIQSLPIAIVAVLGVTLVCPAPLGAQERQAVVRVSPFTAVGVGS
                     SEAAMLERLVVSYIVETRSFRVVDAQGQDRALVETEAALSRGETTAVALPLTADFIVN
                     GSIGRIGELFVLSLEVTRVSDGIMLSVADTAESISDIVLRARASTRRLFGLEPTGGLP
                     TAASPATFQASADLAASATQTQAIDTVEPEASAMFVDRPSVSDISGTWRGDKGLETVR
                     IFPNGAGLAVLSGGGTLKLRVTIRGGEVVIAQDQANDAALYKASSVSFAMAKAIAERA
                     RPMRWVFRLDATRDLLSGMKESVAVSYSGDEQLIDNDNVREASWTRISR"
BASE COUNT          293 a          526 c          487 g          243 t
ORIGIN      
        1 cgagcgcagc ccctcggccg agtcgccgac cgccgaggat agcgcccgaa tccttgatta
       61 gccacccgat cggcgatacc attaagctca agtacgacgc cggaggtacg cgtgcagatc
      121 gaggtcgctt ccatccgaat acgcaagcga atacgtaagg accttcgcga tatcgaccag
      181 ctggcggcca gcatggatcg cttcgggcaa ttgcatccca ttaccgttac ccggcggaac
      241 gtgctcatag ccggcaggcg aaggctcgag gccgccaggc gtctgggctg gtcgaccatc
      301 gaggctaccg tcgtcggcaa gagcgacgcc gcgcagcgcc tggagctcga gatcgaggag
      361 aacatccagc gcttccccct cgacagggac gagatcgagc aggcgctgct caggctcgag
      421 cgcctgcgca accccggttt cctcaggcgc gcctggtacg ccatcctgtc cttcttccga
      481 aggctatttg ggatagacga ccgatgagct ccgagaccgt caggcgaagg gaggtcacat
      541 gatacaatcg cttccgatcg ccatagtcgc agtcctcggc gtcaccttgg tatgccccgc
      601 gccgctaggc gcccaagaac gccaagccgt ggtcagggtc tcgccgttca cggccgtagg
      661 agtcggctcc agcgaggccg cgatgctcga gcgcctcgtc gtctcctaca tcgtcgagac
      721 gcgttcgttc agggtcgtcg acgcccaggg gcaagacagg gccctcgtcg agaccgaggc
      781 ggccctgagc cgcggggaga cgaccgccgt cgccctgccc ctgacggcgg acttcatcgt
      841 caacggctct atcggcagaa tcggcgagct gttcgtgctc agcctggagg tcacgagggt
      901 atcggacggc atcatgctat cggtagcgga taccgccgaa tccatcagcg acatcgtgct
      961 acgcgctcgc gcttccacga ggcggctatt cggcctcgaa ccgacgggcg gcctaccaac
     1021 ggccgccagc cccgcgacct tccaggcttc ggccgaccta gcggcatccg ccacgcagac
     1081 acaggccatc gacacggtcg agcccgaggc gagcgccatg ttcgtcgacc ggccgagcgt
     1141 ctcggacatc agcggcacct ggaggggcga caagggcctc gaaacggtac gtatcttccc
     1201 gaatggcgct ggcctggccg tactgagcgg aggcggcacc ctaaaactgc gcgtgacgat
     1261 acgcggagga gaggtcgtca tcgcccagga ccaagccaat gacgccgcgc tgtacaaggc
     1321 ctcctcggtc agcttcgcga tggccaaggc tatcgccgag cgcgccaggc ccatgcgatg
     1381 ggtcttccgc ctcgacgcga cccgagacct cctgtccggc atgaaggaat cggtagccgt
     1441 cagctactcc ggcgacgagc aacttatcga caacgataac gtccgggagg cttcctggac
     1501 caggatctcg cgctgaccgg cgcgagatcc tggtccagga agcctcccg
//
LOCUS       DAOPHV010000658         1549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_117112_length_1549_cov_2.877510, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000658 DAOPHV010000000
VERSION     DAOPHV010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117112_length_1549_cov_2.877510"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /gene="ptsP"
                     /locus_tag="PLQ29_13095"
     CDS             complement(<1..1526)
                     /gene="ptsP"
                     /locus_tag="PLQ29_13095"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPG87628.1"
                     /translation="MFLDDEEPTIPRYAVTESELDAEWDRFVVAVGKAKDDVEALRDR
                     AVREMGQEHGAIFDSHLIMLDDPDLLESIEASLRSSLLNMEWIVHQYAQAIVKRLEGL
                     DDPVLQERTVDVHDITRRILNHLMFRERFSLADLNSDVVLVARNLLPSDMIAMNRSKV
                     RAIVMDAGGKTSHTAILARAFEIPAVLGLGGSARAIRNGDIVVVDGNTGDVRVQPDPQ
                     TLERYERIRARMAERDETLSGLASLPAKTLDGKEVLIKANIEVPEETDSVLRHGAQGI
                     GLFRSEFLFLQPGRAPSEESQYVAYGRVIEAMGDRPVTIRTLDVGGDKVVPDLAIEEE
                     KNPLLGWRAIRYCLSDKELFQSQLRAILRASVSGDARIMFPMISGPVELDSALAQLEA
                     AKAECRRRGQSFREDIPVGIMIEVPSAAMIADILAPKVDFFSIGTNDLIQYTIAVDRG
                     NERVAYLYEPFHPAVIRLVKKVIDDGHAAGIPVSMCGEMASDPFAAVVLLGLGLDEFS
                     "
BASE COUNT          240 a          508 c          517 g          284 t
ORIGIN      
        1 atcgagaact cgtcgaggcc gaggccgagc agcacgacgg ccgcgaaggg gtcgctggcc
       61 atctcgccgc acatcgacac cgggatgccg gcggcgtggc cgtcgtcgat caccttcttg
      121 accagacgga tcacggcggg atggaacggc tcgtacaggt aggctacccg ctcgttgcct
      181 cggtcgaccg ctatcgtgta ctggatcagg tcgttcgtgc cgatcgagaa gaaatccacc
      241 ttcggggcca ggatgtcggc tatcatcgcc gcgctcggca cctcgatcat gatgccgacc
      301 ggtatgtcct cgcggaagga ctggccccgt cgcctgcact cggccttggc cgcctcgagc
      361 tgggccaggg cagagtcgag ctcgaccggg cccgagatca tcgggaacat gatgcgcgcg
      421 tcgcccgata cgctggcgcg caggatggcc cggagctggc tctggaacag ttccttatcc
      481 gaaaggcagt agcggatggc ccgccacccg agcagggggt tcttttcctc ctcgatggcc
      541 aggtcgggga ccaccttgtc cccgccgacg tcgagggtgc gtatggtgac cggccggtct
      601 cccatcgcct ctatcacccg gccgtaggca acgtactgcg attcctcgct cggggcccgg
      661 ccgggctgca ggaacaggaa ttccgaccgg aacaggccga tgccctgggc tccgtggcgt
      721 aacacagaat cggtctcctc cggcacctct atgttcgcct tgatcagcac ttccttgccg
      781 tcgagcgtct tggccggtag cgaggccagg ccgctcagcg tctcgtcgcg ctcggccatc
      841 ctggctcgga tgcgctcgta gcgctcgagg gtctgcgggt ccggctgcac ccggacgtcg
      901 ccggtgttgc cgtccacgac gacgatgtcg ccgttcctga tggctcgagc cgagccgccg
      961 aggccgagca ccgccggtat ctcgaaggcc ctggcgagga tggccgtgtg gctggtcttg
     1021 ccgccggcgt ccatcacgat ggccctgacc ttggagcggt tcatcgctat catgtccgag
     1081 ggtagcaggt ttcgggcgac caggacgacg tcggagttca ggtcggcgag cgagaagcgc
     1141 tcgcggaaca tcaggtggtt gagtatgcgc ctcgttatgt catgcacgtc taccgtgcgc
     1201 tcctgcagga cggggtcgtc caggccctcc aggcgcttga ctatggcctg ggcgtactgg
     1261 tggactatcc actccatgtt gaggaggctc gaccgcaggg aggcctcgat ggactccagc
     1321 aggtccgggt cgtcgagcat gatcaggtgc gagtcgaaaa tggcgccgtg ctcctgaccc
     1381 atctcgcgga ccgcccggtc gcgtagcgcc tcgacgtcgt ccttggcctt gccgacggcc
     1441 acgacgaacc gatcccactc cgcgtccagc tcggactcgg tgacggcgta cctggggatg
     1501 gtcggctcct cgtcgtcgag gaacatgaag gccgacccta tggctatgc
//
LOCUS       DAOPHV010000659         1546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_117444_length_1546_cov_3.848424, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000659 DAOPHV010000000
VERSION     DAOPHV010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117444_length_1546_cov_3.848424"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PLQ29_13100"
     CDS             complement(<1..745)
                     /locus_tag="PLQ29_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG87629.1"
                     /translation="MNSATMDSSTTIGVPEKLPFWKKLMYALGQLGWSLGSYGAANFL
                     NQYYLPFNEGGEAIYPRMIHQGYVIGVLTVIGLALGFGRVFDAVTDPLIAVMSDRSKL
                     RLGRRRGFLAFSALPFALFSVLVFIPIGGAGAGANIVLNSVWLFACITLLYLFMTMYV
                     TPFFAWMSELGHNADERLQLSTMISITWAMGAMLGAQAPVLQGVFQARGLSALQSFQT
                     TMALFGGVAFLLMMLPVVFIDEKKYCVSIP"
     gene            complement(759..>1546)
                     /locus_tag="PLQ29_13105"
     CDS             complement(759..>1546)
                     /locus_tag="PLQ29_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87630.1"
                     /translation="GYKLLRGMRGDETPKPMPKGSVVYTGHYIDHELVSNLEEDTAAR
                     VSRLQGGQPLRVLLTIGGAGAQRELFKAVIERLLPAVGIGRVCLLINVGDHHEVLDWL
                     RGAVPGLSAAVVHSDDWAGTKRLAAAALAEGASSGSVEGVHLFHDADIFAAVYATNLL
                     MRASDLLVTKPSELAFYPIPKLHLRRVGGHEAWGAIRSAELGDGSVECGSTKETLAML
                     DLLLDDREGLALMNERILSAKRAGVYDGAYRAVDLASGRRENK"
BASE COUNT          274 a          486 c          528 g          258 t
ORIGIN      
        1 tcggtatgga gacgcagtat ttcttctcgt cgatgaagac gacgggcagc atcatgagca
       61 ggaaggcgac gccgccgaag agggccatgg tcgtctggaa cgactggagc gccgagagcc
      121 cgcgagcctg gaagacgccc tggagcaccg gggcttgggc gcccagcatc gcgcccatgg
      181 cccaggtgat ggagatcatg gtcgatagct gcaggcgctc gtcggcgtta tggccgagtt
      241 cagacatcca ggcgaagaac ggcgtcacgt acatggtcat gaacaggtac agcagcgtga
      301 tgcacgcgaa gagccatacg gagttgagga cgatgttggc ccctgctcca gcgccgccaa
      361 tcgggatgaa caccaatact gaaaagagcg cgaacggtag ggccgagaaa gcgaggaagc
      421 cgcggcgcct cccgagcctc agcttggagc ggtctgacat cacggcgatc agggggtcgg
      481 tgaccgcgtc gaagacgcgg ccgaagccga gggccagccc gatgaccgtg agcacgccga
      541 tgacgtagcc ctggtggatc atcctggggt agatggcctc gccgccctcg ttgaacggca
      601 ggtagtactg gttgaggaaa ttagcggctc catagctgcc gagggaccag ccgagctggc
      661 ctagggcgta catcagcttc ttccagaacg gaagcttctc cgggacgccg atcgtcgtgc
      721 ttgaatccat ggtcgcgctg ttcatcgctt ccgtctcctt atttgttctc tcggcggccg
      781 gacgcgaggt ccaccgcgcg gtatgcgccg tcatagacgc cggctcgctt ggccgacagg
      841 atgcgctcgt tcatcagcgc caggccctcg cggtcatcga ggagcaggtc gagcatcgcc
      901 agcgtctcct tggtcgagcc gcattcgacg ctgccgtcgc ccagctccgc tgacctgatc
      961 gctccccagg cctcgtgccc gccgacgcgc ctgaggtgca acttcggtat cgggtagaag
     1021 gccagctcgc tcggcttggt cacgaggagg tcgctcgccc gcatgaggag gttcgtcgcg
     1081 tagacggcgg cgaagatatc cgcgtcgtgg aagaggtgga cgccttcgac gctccccgac
     1141 gacgcgccct cggcgagagc ggccgcggcg aggcgtttcg tgccggccca gtcgtccgag
     1201 tgaaccacgg ccgccgagag cccgggcacg gcgccgcgga gccagtcgag tacttcgtga
     1261 tggtctccga cgttgatgag caggcagacc ctaccgatgc cgaccgccgg caggagacgc
     1321 tcgatcacgg ccttgaagag ctcgcgctgg gcgccagcgc cgcctatcgt cagcagcacg
     1381 cggagcggct ggccgccctg gagcctggag accctggccg ccgtatcctc ctcgaggttg
     1441 gagacgagct cgtggtcgat gtagtggccc gtatacacga cgcttccctt gggcatgggc
     1501 ttcggggtct cgtcgcctcg catgccgcgg agcagcttat agccct
//
LOCUS       DAOPHV010000660         1546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_117453_length_1546_cov_3.543260, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000660 DAOPHV010000000
VERSION     DAOPHV010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117453_length_1546_cov_3.543260"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PLQ29_13110"
     CDS             <1..637
                     /locus_tag="PLQ29_13110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPG87631.1"
                     /translation="ALHDRLCGAEGAFDESVAGIIAMIAAGISVQTNSTLTRANAEAL
                     LGLPAFVVGLGVRRMSMNLFIPTGTGRHGGGLFFPYAEAGAFIDEARRRAHEAGVDLY
                     WYSPMPMCLYNPIARGLGNKSCAACDGLLSVSPSGDVLPCSSWPEPVGNLLSDGFERV
                     WFSEAAAWFKRKRYAPEPCMTCGSFTACQAACPLYWKFAGHDEIKPRAEAR"
     gene            634..1527
                     /locus_tag="PLQ29_13115"
     CDS             634..1527
                     /locus_tag="PLQ29_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87632.1"
                     /translation="MMEIRFRFLKLGLGSRLVAAAALYTAAVGLQLILGSPFFGLPAI
                     LAAWLLLALKPATNKPDDQGLEEWRAVTDGEVSRMADALARSRKLRNGVIGPVAAGVM
                     LLGLLLFGTIVLAAVGSRLWLVTLDLALFSLPGLFFGRIGVHVPAELDLAMGRFLAAM
                     SVPLPEGYAMTPYLRFDKDGQGRDVPENLRLMLEPRRKPDDLVGVQFQAAINKGPNGN
                     VPYMYAVALTRGRSGPAYAQLSGLRLRGYEVEPGGDDEYGTVVVRQDTSGGGYHTTDD
                     DCESLVRLMVKALERTEALTL"
BASE COUNT          228 a          526 c          514 g          278 t
ORIGIN      
        1 ggccctgcac gatagactat gcggcgccga aggcgcgttc gatgaaagcg tcgccggtat
       61 catagccatg atcgccgccg gcatatccgt ccagaccaac tcgacgttga cccgggccaa
      121 cgccgaggcg ctcctcgggc tgccagcctt cgtcgtcggg ctcggcgttc gtcgcatgtc
      181 gatgaacctg ttcattccga ccggcaccgg ccgccatggc ggcggcctct tttttccgta
      241 cgccgaggcc ggcgccttca tcgacgaggc caggcgtcgc gcccacgagg ccggcgtcga
      301 cttgtactgg tactcgccga tgccgatgtg cttgtacaac cccatcgcca gggggctcgg
      361 caataaaagc tgcgccgcct gcgacggtct cctgtccgtc tcgccatccg gcgacgtgct
      421 accgtgctcg tcgtggcccg agccggttgg caacctgctg tcggacggct tcgagcgggt
      481 ctggttctcg gaggcggcgg cctggtttaa gcgcaaacgc tacgcgccgg agccgtgtat
      541 gacctgcggc tcgttcacgg cctgtcaggc cgcctgcccg ctctactgga aattcgccgg
      601 ccacgacgag atcaagccgc gcgcggaggc tcgatgatgg aaatacgttt taggttcctc
      661 aagctgggtc tcggctcccg gctcgtggcg gccgccgccc tgtacaccgc ggcggtcggc
      721 cttcagttga tcctgggctc gccgttcttc ggcctgccgg cgatactcgc cgcctggctt
      781 ctcctggcgc tcaagccggc cacgaacaag cccgacgacc agggcctgga ggagtggcga
      841 gccgtgactg acggcgaggt ctccaggatg gccgacgctc tggcccggtc gcggaagctg
      901 cggaacggcg tcatagggcc cgtagccgcc ggcgtcatgc tgctcggcct gcttctcttc
      961 ggaaccatcg tgctggccgc cgtcggctcg aggctgtggc tggtaacgct cgacctggcg
     1021 ctgttctcgt tgcccgggct gttcttcggg cgtatcggcg tccacgtacc ggcggaactc
     1081 gaccttgcga tgggccgttt cctggctgcg atgagcgtgc cgctaccgga gggctacgcg
     1141 atgacgccat acctacgctt cgacaaggac ggacagggcc gtgacgttcc cgagaacctg
     1201 cgcctgatgc tcgagccgag gcgcaagccg gacgacctgg tgggcgtgca attccaggcg
     1261 gccatcaaca aggggccgaa cggcaacgtg ccctatatgt acgccgtcgc cctgactcgt
     1321 ggccgctcag ggccggccta cgcgcagctg agcgggctgc gactccgcgg ctatgaggtt
     1381 gagccgggcg gcgacgacga gtacggcacc gtcgtggtca ggcaggatac ctccggcgga
     1441 ggctaccata cgaccgatga cgactgcgag tcgctcgtcc ggctcatggt caaagccctc
     1501 gagcgtacgg aggccctgac cctatagcgg gcctccgtct ccgacc
//
LOCUS       DAOPHV010000661         1545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_117557_length_1545_cov_4.073154, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000661 DAOPHV010000000
VERSION     DAOPHV010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117557_length_1545_cov_4.073154"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PLQ29_13120"
     CDS             complement(<1..335)
                     /locus_tag="PLQ29_13120"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPG87633.1"
                     /translation="MDDASLRERIRALVFAAPALSEALASGGFRSVFKGRGMDFEALR
                     EYEATDDALRMDWNATARFGRPFVKTYKDDRNLTLYLIVDESRSMDFGAGRAKRETAA
                     LAASLLAYA"
     gene            complement(335..1348)
                     /locus_tag="PLQ29_13125"
     CDS             complement(335..1348)
                     /locus_tag="PLQ29_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG87634.1"
                     /translation="MDETRSGREASEAYRVIERVRAAMAGRLVGKGEIVDAILTAVMS
                     DGHLLLEGVPGLAKTLAVRTFAAAAGLSYRRIQFTPDLLPGDITGTLFYDAASGAFVP
                     RRGPVFANIVLADEINRAPAKVQSALLEAMEERQVTMGDHSLPLPEPFFVLATQNPIE
                     HEGTFRLPEAQLDRFMLKVVVDYPDEAEELDVVLRYADRPAGKADEAAVQPVIGQAEI
                     AALKAAAASVRFERDAAAYAVAVVRATRPSRDGKQAAGALRELSRYIEYGASPRGSIA
                     LYRAARAAALLAGRDYVLPDDVKRSAPAALRHRVIPSYEAEADRVGPDAIVKGVLSAV
                     PVP"
BASE COUNT          235 a          533 c          562 g          215 t
ORIGIN      
        1 caggcgtagg ccaggagcga ggcggccagg gcggcggtct cgcgcttggc ccggccggcg
       61 ccgaagtcca tcgagcggga ctcgtcgacg atcaggtaga gcgtcaggtt gcggtcgtcc
      121 ttataggtct tgacgaaggg ccgaccgaag cgcgcggtcg cgttccagtc catgcgcagc
      181 gcgtcatcgg tagcctcgta ctcgcggagc gcctcgaagt ccatgccgcg ccccttgaac
      241 accgacctga agccgcccga ggcgagcgcc tcggacaggg ccggcgcggc gaacacgagg
      301 gcgcgtatcc gctcgcgcag cgaagcgtcg tccatcaggg caccggcacg gccgacagca
      361 cgcccttgac gatggcgtcc ggcccgaccc ggtcggcctc cgcctcgtag gacgggatga
      421 cgcggtggcg tagcgccgcc ggagcggatc gcttgacgtc gtcgggcagc acgtagtcgc
      481 ggccggcgag cagcgcggcg gcccgggccg cgcggtacag cgcaatcgag ccgcgcgggc
      541 tggcgccgta ctcgatgtag cgcgacagct cgcgcagggc gccggcggcc tgcttgccgt
      601 cccgcgacgg cctcgtggcc cggaccacgg cgacggcgta ggcggcggcg tcccgctcga
      661 agcgcaccga cgcggccgcc gccttgagcg cggcgatctc cgcctgcccg atgaccggct
      721 ggacggccgc ctcgtcggcc ttgccggctg ggcgatcggc gtagcgcagc accacgtcga
      781 gctcctcggc ctcgtcgggg tagtcgacga cgaccttgag catgaagcgg tccagctggg
      841 cctccggcag cctgaaggtg ccctcgtgct cgatcgggtt ctgcgtcgcc agcacgaaga
      901 acggctcggg caggggcagg ctatggtcgc ccatcgtcac ctggcgctcc tccatcgcct
      961 cgagcagggc agactgcacc ttggcgggcg cgcggttgat ctcgtcggcc agcacgatgt
     1021 tcgcgaagac cgggccgcgc cgcgggacga aggcgccgga ggcggcgtcg tagaacaggg
     1081 tgccggtgat gtcgccgggc aggagatcgg gcgtgaactg gatgcggcgg tacgataggc
     1141 cggccgcggc agcgaaggtc ctgaccgcca gggtcttggc caggccgggc acgccctcga
     1201 gcagcaggtg gccgtcggac atcaccgcgg tcaggatcgc gtcgacgatc tcgcccttgc
     1261 ccaccagccg gccggccatc gccgcccgta cccgctcgat gacgcgatac gcctcggagg
     1321 cttccctgcc cgatctcgtc tcgtccatgc tgcctgctcc atttcccgct ctataggcgt
     1381 cgtcaccgtc catagtatac ggccccggcc cctccggaca agtctgggcg agatcgacgc
     1441 gcccgcgagg ccgatgctag gaacgtctcg cgcggaggaa ctggggagga gggcttgtac
     1501 gacggaatac ggaacatgat ggacaggata aggacaagat gaaca
//
LOCUS       DAOPHV010000662         1541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_118017_length_1541_cov_4.096231, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000662 DAOPHV010000000
VERSION     DAOPHV010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118017_length_1541_cov_4.096231"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /gene="nusA"
                     /locus_tag="PLQ29_13130"
     CDS             <1..1027
                     /gene="nusA"
                     /locus_tag="PLQ29_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPG87635.1"
                     /translation="LGRVEGFFPKKFQSPRETYQPGERIKALIAEVSKNGPQIVLSRT
                     HPEFVQKILELEVPEVYDKTIEVFKIVREPGYRTKIAVYSKRDDVDPVGACVGLKGIR
                     IQSIIRELEGEKIDVLKYEIDPRRFIKNALSPADVREVYIIDEARHTALAVVDESQLS
                     VAIGKQGLNVRLANRLCDWSIDVKTEEQFRELDLSSEARRAASSLFGDEEEILRLDEL
                     PGLDPGLVAALAGAGIEMIEDFLARSEDELLAIEGVAEDALSSLRAVIDENVEVIEEE
                     IEENAVPAEAMEPPPVEQEDVEQETYECPDCHAVITPDMTHCPQCGVALSFEYEDAEA
                     EAEDQES"
     gene            1037..>1541
                     /locus_tag="PLQ29_13135"
     CDS             1037..>1541
                     /locus_tag="PLQ29_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87636.1"
                     /translation="MSDNNENKPKPVLIKQAKPRPAEETAESNPAAPIEKRKVVVVKK
                     KPPVPAAPSAPLAAPSVGQAPARKPAARVVAHHQERADDQAHPAAPTSAPSPQPSTEA
                     PRPAAPQDRPRPSDSSSTNLRTTRPAGTGPRVVGRVGGYRGSSGGYQGGGSSGGYQGG
                     GSSGGYQG"
BASE COUNT          322 a          502 c          493 g          224 t
ORIGIN      
        1 cctcggcagg gtcgagggct tctttccgaa gaaattccag tctccgcgcg agacctacca
       61 gccaggagag cgcatcaagg ccctcatcgc cgaggtcagc aagaacggcc ctcagatcgt
      121 gctgtcccgt actcatcccg agttcgtcca aaaaatactc gagctcgaag tgcctgaggt
      181 ctacgacaag accatagagg tcttcaagat agtgcgcgag ccaggctatc gtaccaagat
      241 agccgtgtac tcgaagcggg atgacgtcga ccccgtcggc gcctgcgtcg gcctcaaggg
      301 catacgcatc caaagcatca tacgcgagct cgagggcgag aagatcgacg tgctcaagta
      361 cgagatcgat ccgcgccgct tcatcaagaa cgcgctgtcc cccgcggacg tgcgtgaggt
      421 ctacatcatc gacgaggcta ggcataccgc cctcgccgtc gtcgacgaga gccagttgtc
      481 tgtcgccatc ggcaagcagg gcctgaatgt acgccttgcc aaccggctct gcgactggag
      541 catcgacgtc aagaccgagg agcagttcag ggagctcgac ctctcgagcg aggccaggcg
      601 tgccgcctcg agcctgttcg gcgacgagga ggagatcctc aggctggacg aactgccggg
      661 tctcgacccg ggcttggtcg cagccctcgc tggcgccgga atcgagatga tagaagactt
      721 cctagccagg tcggaagacg agctgctcgc catcgagggc gtcgcagaag acgccctctc
      781 gagcctgcgg gcggtcatcg acgagaacgt cgaggtcatc gaagaggaga tcgaggagaa
      841 tgccgtaccg gcggaggcga tggaaccgcc tccggtcgag caggaggacg tcgagcagga
      901 gacgtacgag tgcccggatt gccacgcggt cataacgccg gatatgaccc attgcccgca
      961 atgcggcgta gccctctcgt tcgaatacga ggacgcggaa gcggaagcgg aagaccagga
     1021 atcttgaagg tgagctatgt ccgataataa cgagaataaa cccaagcccg tcctcatcaa
     1081 gcaggcaaaa cccagacccg cggaggagac ggccgagtcg aatcccgcgg cgccgatcga
     1141 aaagcgcaag gtggtcgtcg taaagaagaa gccacccgtc ccggcggcgc cttccgctcc
     1201 ccttgccgcg ccgtccgtcg gccaggctcc cgcgaggaag ccggccgcca gggtcgtagc
     1261 ccatcatcag gaaagggccg acgaccaggc tcatccggcc gccccgacct cggcgccgtc
     1321 cccccagcca tccactgagg ctcctcgccc cgccgcgccg caggatagac cgaggccgtc
     1381 cgactcctcg tcgacgaatc tgcggactac gaggcccgcg ggcacgggcc ctcgcgtcgt
     1441 aggcagggtc ggaggctatc gaggctcatc cggcggctac cagggcggcg gctcatccgg
     1501 cggctaccag ggcggcggct cgtccggcgg ctaccagggc g
//
LOCUS       DAOPHV010000663         1538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_118382_length_1538_cov_3.585974, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000663 DAOPHV010000000
VERSION     DAOPHV010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118382_length_1538_cov_3.585974"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLQ29_13140"
     CDS             <1..337
                     /locus_tag="PLQ29_13140"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPG87637.1"
                     /translation="AAELEAMRGAFLVNVGRGDVVDERALYEALRDGVLTGAGIDVWY
                     VYPPKGSVTGAPSRYPIHRLPNVVMSPHVAGSTYEAVDLNIAQTVENLDRWLRTGDAE
                     HRVDLRASY"
     gene            complement(355..>1538)
                     /locus_tag="PLQ29_13145"
     CDS             complement(355..>1538)
                     /locus_tag="PLQ29_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87638.1"
                     /translation="ALEAVGPLHVDTGYVDQDDLDGHTGPVVRLVQPAIAVDDLDVEI
                     AADLVECPADRVLRDAVDAAVENLSRGAQAAVGASLDKDGLERGAGVLWNQGIAVTPE
                     LYLGMPGDDYDSAIATFDYLGMTGMGQDAKLSPMPLAAGSPVRADSSAFGVKEYLESP
                     PYWVIETDWMDEDDLLDAVADYEESFDVALGSPVAPTFKPERGGYVCFADLRSGRGGA
                     DGLDALLVAPERLASSVTLLLDADDPERAARVARAARDLRKENPFCLWQIALRSDSGL
                     PPAGTVRRLADAFSMPEHYFELSRLFALDPQPDYQVRCFFVTGSEALALAALRERPDV
                     ETVFTLGDAMPGAKLLEALPFLAFDREACPFELLYDVMSAYRDYPDLLIEAPRRLFGH
                     E"
BASE COUNT          239 a          528 c          540 g          231 t
ORIGIN      
        1 cgccgccgag ctcgaggcga tgcgcggggc ctttctggtc aacgtggggc gcggcgacgt
       61 ggtcgacgag cgggctctct acgaggctct gcgcgacggc gtcctgaccg gggccggtat
      121 cgacgtctgg tacgtctacc cgcccaaggg ctcggtcacg ggcgcgccct cgcgctatcc
      181 gatccatagg ctgccgaacg tggtcatgtc gcctcacgtg gccggctcga cctacgaggc
      241 tgtcgacttg aacatagccc agaccgtcga gaacctggat cgatggcttc ggaccgggga
      301 cgccgagcac cgcgtcgacc tgcgcgcatc ctattgaagg ctagcggcgt ccggctactc
      361 gtgcccgaat agccttcgcg gcgcctcgat gagcaggtcg gggtagtcgc gataggcgct
      421 catcacgtcg tacagcagct cgaaggggca ggcctcgcgg tcaaaggcca ggaagggcag
      481 ggcctcgagc agcttggcgc cgggcatcgc gtccccgagc gtgaagaccg tctcgacgtc
      541 cggccgctcg cgtagcgccg ccagggcgag cgcctccgag ccggtgacga agaagcacct
      601 gacctggtag tccggctggg ggtcgagggc gaacagccgc gacagctcga agtagtgctc
      661 gggcatcgag aacgcgtcgg cgaggcgcct gaccgtcccc gctggcggca gacccgagtc
      721 ggagcgtagc gctatctgcc agaggcagaa cgggttctcc ttgcgcaggt ccctggccgc
      781 cctggccacc ctggccgcgc gctccggatc atccgcgtcg aggagcaggg tgaccgacga
      841 ggcgagccgc tccggcgcga cgaggagcgc gtcgagcccg tcggcgccgc cgcggcccga
      901 tcgcaggtcc gcgaagcaga catagccgcc gcgctcgggc ttgaaggtcg gggcgaccgg
      961 cgagccgagc gccacgtcga agctctcctc gtagtcggcc accgcgtcga ggaggtcgtc
     1021 ctcgtccatc cagtcggtct ctataaccca gtacggcggg ctctcgaggt attccttgac
     1081 gccgaaggcg gacgagtcgg ctcggaccgg cgagccggct gctagcggca tcgggctcag
     1141 cttcgcgtcc tggcccatgc ccgtcatgcc gaggtagtcg aaggtcgcga tggccgagtc
     1201 gtagtcgtcg ccgggcatcc cgaggtacag ctccggcgtc accgcgatac cctggttcca
     1261 taggacgcca gcgccgcgct cgaggccgtc cttgtcgagc gacgcgccga cggccgcctg
     1321 ggcccctcgg gagaggttct ctacagcagc atcgacggcg tcacgcagca cccggtcggc
     1381 cgggcactcg accaggtccg ccgcgatttc cacgtcgaga tcatctacgg ctatcgccgg
     1441 ctgtacgagc cgcacaacgg ggcccgtgtg gccgtcgagg tcgtcctgat cgacgtatcc
     1501 cgtatcaacg tgcagcgggc caacagcttc aagggccg
//
LOCUS       DAOPHV010000664         1533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_118983_length_1533_cov_3.586604, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000664 DAOPHV010000000
VERSION     DAOPHV010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118983_length_1533_cov_3.586604"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PLQ29_13150"
     CDS             complement(<1..904)
                     /locus_tag="PLQ29_13150"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG87639.1"
                     /translation="MLEELSIRDFAIIDRLSARFEPGLNLLTGETGAGKSILIGAMGF
                     ILGAKSDTGIIRSGAEETLVTAVVSVDGNRDALAWLSERGIEPEDGAVMVRRGLKRSG
                     RGSAYIQNVPVVRQDLQDFSSTLVELHGQRDGHALLKKDRHRQLIDRYAGIDGEVAEY
                     GEAYVELSAKRKALETMAGSEAERAREIEFLRFMIEEIDAAAPRAGEELELEEEERRL
                     SQHEKLFSAVSVARELLSETDGAVARLRKARQQFEIASAIDQRLADQARRLDDAYYEL
                     EDLGLSLATYADQLLFDPARLEEIE"
     gene            complement(897..>1533)
                     /locus_tag="PLQ29_13155"
     CDS             complement(897..>1533)
                     /locus_tag="PLQ29_13155"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPG87640.1"
                     /translation="APFGVPILPVNLGTLGFIAWVKRDEWKERFDEAVSGGLAVSERF
                     MLDVSVERDGKAVAGFSALNDGVISGSGPAKIVELCVSVNGARLGKYRSDGLIVSTPT
                     GSTAYSLASGGPVLDPDMEAMVFTPICPFTLSNRPLVLPGGESLELYVERGQRERIIL
                     TVDGQEFYDLQEGDRVSFRRSAFKARLFRHERAAFFQVLRSKLNWSGGPDA"
BASE COUNT          268 a          497 c          479 g          289 t
ORIGIN      
        1 attctatctc ctcgagacgg gccgggtcaa acaggagctg atccgcgtag gtagccagcg
       61 acagccccag gtcttccagc tcgtagtacg cgtcgtcgag gcgtctggcc tggtcggcga
      121 ggcgctggtc gatggcgctg gcgatctcga attgttgtct ggccttcctg agcctggcta
      181 cggcgccatc ggtctccgat aagagctcgc gcgctaccga gacggcggag aacagtttct
      241 cgtgctggga gaggcgacgc tcctcttcct cgagctcgag ctcttctccg gcccgaggcg
      301 cagccgcgtc gatctcttct atcataaagc gaagaaattc tatctcacgc gcccgctccg
      361 cctctgagcc cgccatcgtc tccagggcct tgcgcttggc gctcagctcg acgtaggcct
      421 ctccgtattc cgccacctcg ccatcgatgc ccgcatagcg gtctatcagc tgtcggtggc
      481 ggtccttctt gagtagggcg tggccgtccc gctggccgtg cagctcgacg agagtcgacg
      541 agaagtcctg cagatcctgc ctgacgacgg gtacgttctg gatataggcc gaaccccttc
      601 cggagcgctt aaggccgcgg cgtaccatga ccgcgccgtc ctcgggttct atgccccgct
      661 ctgacagcca agcgagcgcg tcccggttgc cgtctaccga cacgacggcc gtcacgagag
      721 tttcttcggc gccggagcgt atgatcccgg tatctgactt ggcccccagg atgaagccca
      781 tcgcgcctat caggatggac ttgccggcgc cggtctctcc ggtcagtaga tttaggcccg
      841 gctcgaagcg agccgacagc cggtcgatga tggcgaaatc gcggatggac agttcctcaa
      901 gcatcggggc ctcccgacca attcagcttc gacctcagga cctggaagaa cgccgcgcgc
      961 tcgtggcgga acagtcgcgc cttgaaagcc gaccggcgga agctgacgcg atcgccctcc
     1021 tggaggtcgt agaactcctg gccgtcaacc gtgaggatga tgcgctcgcg ttgcccgcgc
     1081 tcgacgtaca gttcgaggct ctccccgccg ggtagcacga ggggacggtt cgatagggtg
     1141 aaggggcaga tgggagtaaa gaccatcgct tccatgtccg ggtcgaggac cggcccgccg
     1201 gacgctagcg aataggcggt cgaacccgtc ggagtcgaga ctatgaggcc gtcggagcgg
     1261 tatttgccta gcctagcccc atttaccgaa acgcatagct cgacgatttt ggcagggccg
     1321 gaaccggata tgacaccgtc gttgagggcg ctgaagcccg ctacggcctt gccgtcgcgc
     1381 tcgaccgaca cgtcgagcat gaagcgctcc gataccgcga ggcccccgga taccgcctca
     1441 tcgaagcgtt ctttccattc gtcgcgcttc acccaggcta tgaagccgag cgtgcctaga
     1501 ttaaccggca gtatcggaac gccgaacggg gcg
//
LOCUS       DAOPHV010000665         1530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_119321_length_1530_cov_5.224407, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000665 DAOPHV010000000
VERSION     DAOPHV010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119321_length_1530_cov_5.224407"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..>1530
                     /locus_tag="PLQ29_13160"
     CDS             153..>1530
                     /locus_tag="PLQ29_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HPG87641.1"
                     /translation="MRIARRGGSASLALAAFLTLAGASASAQGVYVVPIHGDIAASTV
                     VFVRRQATKAIGDGAAAIIFDIDTFGGRVDSALRISSFIGSIREADTVAYVRSGPDGL
                     GVSWSAGALIAMSCGSLYMAPGTSIGAAAPVVAGADGSTTRADEKTVSAVRAQMAALA
                     EKNGYPPAIALAMVDADVELYEVRRDGAVALATADELGLEKGADGVPVDPASGERLRL
                     VSAKGKLLSLTAGEAERYGLSSGTAEGLENLLEAVGLEGPAIELTPSVADGLVVFLSS
                     AAVQSLLILVGLVAVFIEINSPGFGIPGTVALVAFMTLFGVNALMGTVGSLELVLFML
                     GAALLAVELFLIPGFGITGIAGILLIAVSLVLSMQDFALPTLDWEWELLGRNALTVAV
                     GILSAIAGIGVLALAGPRLRMFDRLTLHTTIGGTAGGQSLPPAVPEAPETSPAAADSL
                     AGRRGVALT"
BASE COUNT          209 a          553 c          518 g          250 t
ORIGIN      
        1 cgtcgaccgt ggcggctcgg ccaacgagct cagctcgacc gacggcaccg gccccgccag
       61 cgccccggtg atgcacggca ttccctgcgc cttaaagaag atccgcccga ccgtcccttg
      121 agtttcagcc ggaagcgact atagtaaaag gcatgagaat agctcgtcgc ggcggttcgg
      181 cgtctctggc gctggccgcg ttcctaacct tggccggagc ctccgcctcg gcccaggggg
      241 tctatgtcgt cccgatccac ggcgatatag cagcgtccac ggtcgtcttc gtccgccgcc
      301 aggcgacgaa ggccatcggc gatggggccg cggccatcat cttcgacatc gataccttcg
      361 gcggccgcgt cgactccgcc ctacgcatct cgtcgttcat aggctcgatc cgcgaggccg
      421 acaccgtggc ctacgtccgc tccggccccg acggcctcgg cgtctcgtgg tcggccgggg
      481 cgctcatcgc catgtcctgc ggctcgctgt acatggcgcc gggcacatcg ataggggcgg
      541 ccgccccggt ggtcgcgggc gcggacgggt cgacaacacg ggccgacgag aagaccgtca
      601 gcgccgtgcg agcccagatg gccgccctgg ccgagaagaa cggctatccc ccggccatcg
      661 ccctcgcgat ggtcgacgcg gacgtggaac tctacgaggt ccggagggac ggggcggtcg
      721 cgctggccac cgcggacgag ctaggcctcg agaagggagc cgacggcgtc cccgtggacc
      781 cggcctctgg cgagaggctc aggctcgtca gcgccaaggg caagctcctg tcgcttaccg
      841 ccggcgaggc ggagcgctac ggcctatcgt ccggcaccgc cgaggggctc gagaacctgc
      901 tggaggccgt cggcctcgag gggccggcca tcgagctgac cccgagcgtc gcggatgggc
      961 tcgtcgtctt cctgagctcg gcggccgtcc agtcgctgct gatactcgtc ggcctcgtgg
     1021 ccgtgttcat cgagatcaac tcgcccggtt tcgggatacc cggcaccgtg gcgctcgtcg
     1081 ccttcatgac gctgttcggc gtgaacgccc tgatgggcac ggtcggctcg ctcgagctgg
     1141 tgctcttcat gctcggcgcg gccctgttgg ccgtggagct cttcctgata ccgggcttcg
     1201 gtatcaccgg catcgccggc atcctgctca tcgccgtctc gctcgtgctg tcgatgcagg
     1261 atttcgcgtt gccgaccctc gactgggaat gggagctgct cgggcgcaac gcgctcacgg
     1321 tggcggtcgg catcctgtcg gccatcgccg gcataggtgt gctcgccctc gccggacctc
     1381 gcctgcggat gttcgaccgg ctgaccctgc acacgacgat aggcggcacc gccggcgggc
     1441 agtcgctccc gcccgccgtc cccgaggccc cggaaacctc accagccgcg gccgacagcc
     1501 tcgcaggcag gcgcggcgtc gcgctgacca
//
LOCUS       DAOPHV010000666         1529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_119538_length_1529_cov_2.090231, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000666 DAOPHV010000000
VERSION     DAOPHV010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119538_length_1529_cov_2.090231"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLQ29_13165"
     CDS             complement(<1..194)
                     /locus_tag="PLQ29_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPG87642.1"
                     /translation="MNRLRSMNIMMVIYVFSIMMLAGIVTSLSFVALYAMGVRPTIVA
                     APMLSPLFALLVSSVIGTSIS"
     gene            complement(191..865)
                     /locus_tag="PLQ29_13170"
     CDS             complement(191..865)
                     /locus_tag="PLQ29_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPG87643.1"
                     /translation="MFSILVVEDDDNARRLMETVLRRGGYAVTCAADGAVALERMRER
                     HVDLAVVDLMMPRMDGYEFTEALRRYRDETPVLMVTAMEGRADKRRGFVSGADDYMVK
                     PVDEEELLLRIKALLRRARIASERRLEVGGTTLDYDAMTVARDGQAAELPAKEFMLLY
                     KLLSYPGKIFTRRALMDELWDDNPDSDQRTVDVHVNRLRDRFSGNPDFQIVTVRGLGY
                     KAAVSS"
     gene            1023..>1529
                     /locus_tag="PLQ29_13175"
     CDS             1023..>1529
                     /locus_tag="PLQ29_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPG87644.1"
                     /translation="MGKAIGIDLGTTNSCVAIMEGGDAVVLPNAEGGRTTPSVVAFTA
                     AGERLVGQAARRQAAINHGRTISSIKRDMGSDRRVAIDGAEYSPQELSAMILQKLKAD
                     AEAYLGEPVREAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGVDKD
                     YGQKVMVYD"
BASE COUNT          281 a          528 c          477 g          243 t
ORIGIN      
        1 gagatcgagg tgcctatcac gctgctgacg agcagggcga agagcggcga caacatcggc
       61 gcggccacga tcgtcggcct cacgcccatc gcgtacagcg ccacgaagga cagcgaggtg
      121 actatccccg cgagcatcat gatggagaat acgtagatga ccatcatgat gttcatcgac
      181 ctgagcctat tcacgacgag accgccgcct tgtagcccag cccgcgcacg gtgacgatct
      241 ggaagtccgg gttaccggag aagcggtcgc gcaggcggtt cacgtgcacg tccaccgtgc
      301 gctggtcgga gtcggggtta tcgtcccaca gctcgtccat cagcgcccgc ctggtgaaga
      361 tcttgcccgg gtaggacagg agcttgtaga gcagcatgaa ctccttagcc ggcagctccg
      421 ccgcctgacc gtccctcgcc acggtcatcg cgtcgtaatc gagcgtcgtg ccgccgacct
      481 cgagcctgcg ctcggaggcg atgcgtgccc gcctgaggag cgccttgatc cgcaggagca
      541 gctcctcctc gtccaccggc ttgaccatgt agtcgtccgc gccggacacg aagccgcgcc
      601 gcttatcggc ccggccctcc atcgcggtga ccatcagcac cggcgtctcg tcgcgatagc
      661 gcctgagcgc ctcggtgaac tcgtagccgt ccatccgcgg catcatcagg tcgacgacgg
      721 ccaggtcgac gtggcgctcc cgcatgcgct cgagcgccac cgccccgtcg gccgcgcagg
      781 tcactgcgta gccgccgcgg cgcagcaccg tctccatgag gcgcctcgcg ttatcgtcgt
      841 cctcgaccac aagaatgctg aacatatccc tccgagccat cctactatac gaccaacaag
      901 cttcgtacca ctactacata gtcgttattc cggggcgatt cgccaagccg tttacattca
      961 gttcatcttg ggtcgctatc ctatccacgt aacggcgccg gctggcgccc cgaggagagt
     1021 acatgggcaa ggctataggc atagacctgg gcacgaccaa ttcgtgcgtg gctatcatgg
     1081 agggcggcga cgccgtcgtg ctgccgaacg cggagggcgg gcgcacgacg ccgtcggtcg
     1141 ttgcgttcac cgcggccggc gagcgcctcg tcggtcaggc cgccaggcgc caggcggcga
     1201 tcaaccatgg gcgcacgata tcgtcgatca agcgcgacat gggaagcgac cgccgcgtcg
     1261 cgatcgacgg cgccgagtac tcgccccagg agctgtcggc gatgatactc cagaagctca
     1321 aagccgacgc cgaggcctac ctgggcgagc cggtccgcga ggcggtgatc accgtgccgg
     1381 cctacttcaa cgacgcccag cgccaggcga ccaaggacgc cggccgcatc gccgggctcg
     1441 aggtaaagcg catcatcaac gagccgaccg ccgccgccct cgcctacggc gtcgacaagg
     1501 attacggcca gaaggtgatg gtctacgac
//
LOCUS       DAOPHV010000667         1522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_120265_length_1522_cov_4.280845, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000667 DAOPHV010000000
VERSION     DAOPHV010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120265_length_1522_cov_4.280845"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLQ29_13180"
     CDS             complement(<1..651)
                     /locus_tag="PLQ29_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87645.1"
                     /translation="MEAKLAPWRSGRVGFIAGLSMAAVLVGAGVYLGVAVSSAPLAPA
                     VESSFSSYVEGLSATGKIVVVEARERLEIRRTTPGLLFGDTTVGRLLGIRSDATVEAS
                     AWAELSFIIDLKATESWSVRYDPADGGRLSVAAPPIAMLTPAIHTDTITILTTDRSVF
                     LDERLLEARVMRDLTARFVEAASAMLDEPELRSKAAAAIEGIARSFSRTAGFPLASV"
     gene            complement(701..1231)
                     /locus_tag="PLQ29_13185"
     CDS             complement(701..1231)
                     /locus_tag="PLQ29_13185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPG87646.1"
                     /translation="MRILVVSDTHGDFRGLVAVLGLLGRSVQALIHLGDGSGDVKAAM
                     RYGAPTPPAFVVRGNVDADSSVPLARRFDAEGRLLILAHGHRYPLGDGTSSLVYAAKE
                     AGARAFFFGHTHVPYCEERSGVLLLNPGSLSRPRGPWGPSLAIVEAPPSSSCLDVKLF
                     ELVGPQAAPRLRAIRL"
     gene            complement(1228..>1522)
                     /locus_tag="PLQ29_13190"
     CDS             complement(1228..>1522)
                     /locus_tag="PLQ29_13190"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPG87647.1"
                     /translation="YLYRGSVEIGLPPAAGEAGAVLSTVGEGELFGEMAWLLEEPRSA
                     RAVAKTDCMVFVLPPEMFERYLAQDAGASRRLVELMAARLKANNERVGRSGER"
BASE COUNT          242 a          526 c          529 g          225 t
ORIGIN      
        1 taccgacgcc agcggaaagc cggcggtccg cgagaacgac ctggctattc cctcgatcgc
       61 ggcggccgct ttggaccgca gctccggctc gtcgagcatc gccgaggccg cctcgacgaa
      121 gcgcgccgtc aggtcgcgca tgacgcgggc ctcgagcagc cgctcgtcga ggaatacgga
      181 acggtcggtg gtgaggatcg tgatcgtatc ggtatgtatg gccggcgtca gcatcgcgat
      241 cggcggcgcg gcgacgctga gcctgcctcc atcggccggg tcgtagcgta ccgaccagga
      301 ttccgtggct ttcaggtcga tgatgaacga cagctccgcc caggccgagg cctcgacggt
      361 ggcgtcggag cggatgccga gcagtcggcc gaccgtcgta tcgccgaaca ggagccccgg
      421 cgtcgtcctg cggatctcga gccgctcgcg ggcctcgacg acgactatct tgccggtggc
      481 cgatagaccc tcgacgtagg agctgaacga gctctccacg gccggggcaa gtggggcgct
      541 cgatacggct acgcctaggt atactccggc tcccacgagg acggcggcca tcgagagccc
      601 ggctatgaac ccgactcgtc cggatcgcca cggggctagc ttcgcttcca taaccggtac
      661 tatccagctt ttatgccctg ggcgcaagtc cggctccggg ctatagccgt atcgcccgga
      721 gcctcggggc cgcctggggg ccgaccaact cgaacagctt gacgtcgagg cagctcgacg
      781 acgggggcgc ctcgacgatg gccaggctcg gtccccacgg cccgcgcggg cgggataggc
      841 tgccgggatt gaggaggagt accccggatc gttcctcgca gtacgggacg tgggtgtggc
      901 cgaagaagaa ggcccgcgcg cctgcctcct tggccgcgta gacgaggctg gaggtgccgt
      961 cgccaagggg gtagcggtgg ccgtgcgcca ggataaggag ccgtccctcg gcgtcgaagc
     1021 ggcgagcgag cggcaccgac gagtcggcgt cgacgttgcc ccgtacgacg aaggccggcg
     1081 gcgtcggggc tccgtagcgc atcgcggcct tcacgtctcc cgacccgtcc ccgaggtgga
     1141 tcagggcctg gaccgaacgt ccgagcaggc cgagcaccgc gacgagtccc cggaaatccc
     1201 cgtgggtatc cgagacgacc agaatcctca tcgctcgccg gaccttccga cgcgctcgtt
     1261 gttcgccttg agcctggccg ccatcagctc gaccagccgg cgcgacgcgc cggcgtcctg
     1321 ggccaggtat cgctcgaaca tctcgggtgg caacacgaat accatgcagt cggtcttggc
     1381 gaccgctcgc gccgacctgg gctcctcgag tagccaggcc atctcgccga agagctcgcc
     1441 ctcgccgacc gtcgacagga cggcgcccgc ctcgccggcg gccgggggca gccctatctc
     1501 caccgagccc cggtacaggt ag
//
LOCUS       DAOPHV010000668         1521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_120381_length_1521_cov_5.068895, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000668 DAOPHV010000000
VERSION     DAOPHV010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120381_length_1521_cov_5.068895"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLQ29_13195"
     CDS             complement(<1..598)
                     /locus_tag="PLQ29_13195"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPG87648.1"
                     /translation="MTETVDILGLGFEAFFSASGLVSGKRPNLRAAYRAAFRDGRWEP
                     GRFLSRADAEALFPRFRVGLLEAARVASESGEAGTTAKVVFRLDDGQSVESVRIPSPG
                     GGATLCLSSQVGCRMGCAFCETGRAGLARNLTAAEIVSQAVSARVALGWEFSNVVFMG
                     MGEPLDNFDGLAGALAVMFDQSALGLAQERVTVCTVGLP"
     gene            complement(675..>1521)
                     /locus_tag="PLQ29_13200"
     CDS             complement(675..>1521)
                     /locus_tag="PLQ29_13200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HPG87649.1"
                     /translation="TGGCGCIQLAKRKNGLEAEILRCAAAAGELASLPLFDCIGSMES
                     LYGLAKASRRAESGGGALDAEAPDDELLVLVSGEASLTYGGIAFENLTPGAALGDERT
                     LCRSMRLFSAVGRGPTTWLAFPRAVVETRPALLWRLREGLERRLAIVKAVFEFRWRPE
                     YELGITAMDEQHRRIFGAMDELTGAAENDAILVLERLEDLLADHFSDEEALQAAVGYP
                     GREEHAREHAKLLSELSEFMERIRTGVVATGLSNFMKDCFIRHTLLEDRKYAPWVHDG
                     DPRRS"
BASE COUNT          261 a          544 c          494 g          222 t
ORIGIN      
        1 ccggcaggcc cacggtgcag acggtcacgc gctcctgggc caagccgagg gccgactggt
       61 cgaacatcac ggcaagcgcc ccggccagcc cgtcgaagtt gtccagcggc tcgcccatgc
      121 ccatgaacac gacgttcgag aattcccagc cgagcgcgac cctggcgctc accgcctggg
      181 acacgatctc cgctgccgtc aggttccgcg cgaggccggc gcgtccggtc tcgcagaagg
      241 cgcaccccat gcggcaaccg acctgggacg acaggcacag cgtcgccccg ccgccgggcg
      301 atggtatgcg cacgctttcg acgctttggc cgtcgtcgag cctgaagacg accttggccg
      361 tcgtccccgc ctcgccgctc tccgaggcga cgcgggccgc ctccagcagc ccgacgcgga
      421 agcgcgggaa caacgcttcc gcatcggcgc gagacaaaaa gcgccccggc tcccagcggc
      481 cgtcgcggaa ggccgcccga tatgcggcgc gtaggttggg gcgctttccc gagacgaggc
      541 cgctggcgga gaagaaggcc tcgaagccca ggcccaggat gtctacggtc tcggtcatcg
      601 cgcgagcata gcgtatatgg gtatcccggg tcatccggcc gcggcggacg aagcccggcg
      661 gaaacggccg gcgttcacga cctgcgcgga tccccgtcgt gtacccaagg cgcgtacttg
      721 cgatcctcga gtagcgtatg gcggatgaag caatccttca tgaaattcga caagccagtg
      781 gcgacgacgc ccgtacggat acgctccatg aattcggata gctcgctgag aagcttcgcg
      841 tgctcgcggg cgtgctcctc gcggccgggg tagccgaccg ccgcctgtag cgcctcctcg
      901 tcggagaaat ggtcagccag cagatcctcg agacgttcca ggaccaagat cgcgtcgttt
      961 tccgccgcgc cagtcagctc gtccatcgcc ccgaagatgc ggcgatgttg ctcgtccata
     1021 gcggtaatgc cgagctcgta ctcgggtctc cagcggaact cgaagaccgc cttgacgatg
     1081 gccagccggc gctcgagccc ctcgcggagc cgccacagta gcgccggcct cgtctccacg
     1141 acggcgcgcg ggaacgccag ccaggtcgta ggcccgcgcc caacggccga aaacagccgc
     1201 atcgatctgc agagcgtacg ttcgtcgccg agcgccgcgc ccggggtcag gttctcgaag
     1261 gcgatgccgc cgtaggtcag cgaggcctcg ccggagacga gcacgagtaa ctcgtcgtcc
     1321 ggcgcctcgg catcgagggc tccgccgccc gactctgcac ggcgggaagc cttcgccagc
     1381 ccatagaggc tttccatcga gccgatacag tcgaaaagcg gcagcgaggc cagctcaccc
     1441 gcggcggccg cacagcgtag tatctcggcc tcgaggccgt tcttcctctt tgcgagctgg
     1501 atacatccgc atccgcccgt g
//
LOCUS       DAOPHV010000669         1519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_120618_length_1519_cov_4.245219, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000669 DAOPHV010000000
VERSION     DAOPHV010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120618_length_1519_cov_4.245219"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..766)
                     /gene="rsmG"
                     /locus_tag="PLQ29_13205"
     CDS             complement(74..766)
                     /gene="rsmG"
                     /locus_tag="PLQ29_13205"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPG87650.1"
                     /translation="MNETLLTRGLAELGLADDRTASLLGRYVASIEAWNPAYGLVGAS
                     GDELVIKHILDSLAPLAIVRRSLAEAATGTAGYGERASLVDLGTGAGLPGVPLAIAMP
                     EVDVTLLDRMTRRIRFLEAVRAELALENVDIVEEQVERAKGSYDVVTFRAFRPFERKL
                     FKRVFSVCSPGGRVVAYKGKADRACAELEAIDGLYESAEITPVVVPFLADERCVVVMR
                     PKASPTASPRKP"
     gene            complement(804..>1519)
                     /locus_tag="PLQ29_13210"
     CDS             complement(804..>1519)
                     /locus_tag="PLQ29_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87651.1"
                     /translation="KILLTSHVYQRLKGAGVSIQQVDGINKVDFFNTGAIEFKGISLT
                     VYDTVFLDTEGYRLGYREAMENLYVAIDKGMWKSSIFETAATLAEAIIDNLPGGEKGG
                     ASRRSMLDDLKAATDSFGVERYEKAVASFGELVRKLEALPGMDGLALEYLRGVHDNYT
                     ALVDAFTARLDRDIDEHLDTVFGQKELERFRLYRQHHALYGELREQTRLQARSRKVIW
                     FREADRISPELSVRIMARK"
BASE COUNT          242 a          495 c          499 g          283 t
ORIGIN      
        1 ctcgtggggc aacccgctcg tcgagcggga gttcatggtt acggagcgtg aggatacgta
       61 gtcggtagtc ggcctagggc ttgcgcgggc tagccgttgg gctagccttt ggccgcatga
      121 cgacgacgca gcgctcgtcg gccaggaacg gaacgacgac cggcgttatt tctgcgctct
      181 cgtagagccc gtctatcgcc tcgagctcgg cgcaggcgcg gtcggccttg cccttgtacg
      241 cgacgacgcg gccgcctggc gagcagaccg agaagacgcg cttgaacagc ttccgctcga
      301 aaggcctgaa ggccctgaag gtgactacat cgtaagaacc cttggcgcgc tcgacctgct
      361 cctcgacgat gtcgacgttc tcaagcgcta gctcggcgcg gaccgcctcc aggaaacgga
      421 tgcgcctggt catgcggtcg agcagggtca cgtcgacctc cggcatcgct atggccagcg
      481 gcacgccggg caggccggcc ccggtcccga ggtcgacgag actggcccgc tcgccgtagc
      541 ccgcggttcc ggtcgccgcc tcggcgagcg atcgcctgac gatggccaac ggcgccaggc
      601 tgtcgaggat atgcttgatg acgagctcgt cgcccgaggc tccgacgagg ccgtaggcgg
      661 ggttccaggc ctcgatcgag gcgacgtagc gaccgagcag gcttgcggtc cggtcgtccg
      721 cgaggccgag ctcggccagc ccccgggtaa gcagcgtttc gttcacggct cgaatgtagc
      781 ctgaaagagg gccgcccgca tagttacttc ctggccatga tgcggacgct cagctcaggc
      841 gagatcctat cggcctcgcg gaaccagatg accttgcggg agcgcgcctg cagcctggtc
      901 tgctcccgga gctccccgta cagggcgtgg tgctggcggt atagcctgaa gcgttccagc
      961 tccttctgcc cgaagaccgt gtcgaggtgc tcgtcgatat cgcggtcgag cctggcggtg
     1021 aacgcgtcga cgagcgccgt ataattatcg tgtacccccc gcaggtactc gagcgccaat
     1081 ccgtccatcc cgggtaacgc ttcgagcttg cgcaccagtt cgccgaacga cgcgaccgct
     1141 ttctcgtagc gctctacccc gaagctatcg gtggcggcct tgaggtcgtc gagcatcgag
     1201 cgcctcgagg cgccgccctt ctcccctccg ggcaggttgt cgatgatggc ctccgccagg
     1261 gtcgcggccg tctcgaagat gctcgacttc cacatgccct tgtcgatggc tacgtacagg
     1321 ttttccatcg cctcgcggta gccgagcctg tagccctcgg tatcgagaaa gaccgtgtcg
     1381 tagaccgtca ggctgatgcc cttgaactcg atggcgccgg tattgaagaa gtccaccttg
     1441 ttgatcccgt cgacctgctg gatcgagacg cccgcgccct tgagccgctg gtagacgtgg
     1501 ctggtcagga gtattttgt
//
LOCUS       DAOPHV010000670         1518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_120775_length_1518_cov_3.125085, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000670 DAOPHV010000000
VERSION     DAOPHV010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120775_length_1518_cov_3.125085"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..940
                     /locus_tag="PLQ29_13215"
     CDS             80..940
                     /locus_tag="PLQ29_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87652.1"
                     /translation="MYFNELVAAYDRERHERDTFHDLMRHRVREILLVASLYDAFVLE
                     SDGALSEQIYGEFFKLNLTTAPRVSCAYTPESAMELFGSGGYDMVILMAGLDFDVPLA
                     LASRMKALWPSVPILLLAMNNSSLSGLDAGSKACRDVIDRVFVWNGYSKLFIGMIKYV
                     EDLWNVDNDTRVGMVRVVLLIEDSIRYYSRYLPLLYSVVMKQTQQLVEEERVVETYKI
                     LRMRGRPKVLLATSYEEALTLYERYEPYLLTVISDVRYGREGLEDPEAGFRFLEMTKA
                     RKPDLPVLIQ"
     gene            1064..>1518
                     /locus_tag="PLQ29_13220"
     CDS             1064..>1518
                     /locus_tag="PLQ29_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87653.1"
                     /translation="MFRTPEGVELSRARNMEEFVEKMRAVPPECLLYHATRNHFSAWL
                     MARGEVRFARIIRNYLPEDFAGPTELRDFICRALDDLQRGKARGVIPAIGANFGDRGL
                     VRLGGGSVGGKGRGLAFIKSLIDNLAFPNRGGGVDIRLPYTTFIGIDEF"
BASE COUNT          267 a          511 c          476 g          264 t
ORIGIN      
        1 gaggcgctca acgaggcgct caacgaggcg ctcgcgggcg tctcttgcga ggggcgcgga
       61 gccgcgctac gatagcgcca tgtacttcaa cgagctggtc gccgcgtacg accgggagcg
      121 ccacgagcgc gacaccttcc acgacctgat gcgccaccgc gtgcgcgaga tcctgctcgt
      181 cgccagcctc tacgacgcct tcgtgctcga aagcgacggc gccctgtcgg agcagatcta
      241 cggcgagttc ttcaagctca acctgaccac cgccccgagg gtgtcctgcg cctatacacc
      301 cgagtcggcc atggagctgt tcggctccgg cggctacgac atggtcatcc tgatggccgg
      361 cctcgacttc gacgtgccgc tggccctcgc ctccaggatg aaggccctct ggccgtccgt
      421 gccgatactg ctgctcgcga tgaacaactc gagcctttcc ggcctcgacg ccgggtcgaa
      481 ggcctgtcgc gatgtgatcg accgcgtgtt cgtctggaac ggctattcga agctcttcat
      541 cggcatgatc aagtacgtcg aggacctctg gaacgtcgat aacgacacca gggtcggcat
      601 ggtacgggtc gtgctgctca tcgaggactc gatacgctac tactcgcgct acctgccgct
      661 cctgtactcg gtcgtgatga agcagacgca gcaattggtc gaggaggaac gggtcgtcga
      721 gacctacaag atcctgagga tgcgcggtcg tcccaaggtc ctgctcgcga cgagctacga
      781 ggaggcgctg actctctacg agcgttacga gccctacctc ctgaccgtca tctcggacgt
      841 acgctacggg cgcgaagggc tcgaggatcc cgaggccggc ttccgcttcc tcgagatgac
      901 caaggcccgc aagccagacc tgccggtgct gatccagtag agcgaggcga ccaatcgcga
      961 caaggcgtac gcgctcggcg cgtcttttgc agataagaac tcgaacaccc tggccaggga
     1021 actcggcgcg ttcttccagg cccagctcgg cttcggcccc ttcgtgttca ggacgcccga
     1081 gggcgtcgaa ctgtccaggg ccaggaacat ggaggaattc gtcgagaaga tgcgggccgt
     1141 accgcccgag tgcctcctgt accacgcgac ccgcaaccac ttctccgcct ggctgatggc
     1201 caggggcgag gttcgcttcg cccgcatcat ccgcaactac ctgcccgagg atttcgccgg
     1261 gccgaccgag cttcgcgact tcatctgccg agccctcgac gacctgcagc gcggcaaggc
     1321 gcgcggcgtc atcccggcca tcggcgccaa tttcggcgac cgtggcctgg tcaggctcgg
     1381 cggcggctcg gtcggcggca aaggccgcgg gctggccttc atcaagagct tgatcgacaa
     1441 cctggccttc ccgaaccggg gcggcggcgt cgacatcagg ctgccgtaca cgacgttcat
     1501 aggcatagac gagttcga
//
LOCUS       DAOPHV010000671         1514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_121241_length_1514_cov_4.446196, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000671 DAOPHV010000000
VERSION     DAOPHV010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121241_length_1514_cov_4.446196"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1514)
                     /locus_tag="PLQ29_13225"
     CDS             complement(<1..>1514)
                     /locus_tag="PLQ29_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87654.1"
                     /translation="IPLDEWDALLAWCPEELREAALAALASPGAGTGLLSRLEAFTLP
                     GAQEAVVTRREGKAALIEAASRPDGQAALAARWLPYAGPVTLGRLGCLFGPGAAEPGL
                     LEAAGVVVGPGLGVQPRSRSGEPAVEAPSVSAHAADAPGACDRDVLESLLRQGRAATR
                     PKVRARPARDLPAFVAALQGLRDRLLPDAEPAPDDAMAGLAATRRALETLSGYPAPAG
                     LWETEILPARVPGYRPEYLDRLLAEGEFIWFGAGERVVAFAAAADYEVFAPGTPSALL
                     SPRDGPLDAWAIKARHGLSIAELEERLWAELWAGAIGSDGFEAVRLAAGRGFKRVATA
                     SPADPIGTDRRPTRGKPTRVPSALRARWKDGPVLVGAWFALALDDEGPADEADLLELD
                     CRAVRAVVRRYGVLFPAMLDRELPRARWGNLARALRRMELSGELACGRFFEGPDGPQF
                     IGSDALRLFTDGFDDRLASMNACDAASPAGWPIGGLPEGLPSRLPVNRLSTAGD"
BASE COUNT          202 a          550 c          581 g          181 t
ORIGIN      
        1 ctatcgccgg ccgtcgatag gcggttcacc ggcaggcgcg acggtaagcc ctcgggcagg
       61 ccgccgatgg gccaaccggc cgggctcgcg gcgtcgcagg cgttcatcga cgcgaggcgg
      121 tcgtcgaagc cgtcggtgaa cagcctgagc gcgtcggagc cgatgaactg agggccgtcg
      181 gggccttcga agaaacggcc gcaggccagc tccccggaca gctccatgcg cctgagggcc
      241 ctcgcgaggt tgccccagcg agccctcggc agctcccggt cgagcatggc cgggaagagg
      301 acgccgtagc gcctgactac cgcccggacc gcgcggcagt ccagctcgag gaggtcggcc
      361 tcgtcggcgg ggccttcatc gtcgagcgcg agagcgaacc aggcgccgac cagaacgggg
      421 ccgtccttcc agcgggcgcg cagcgccgac ggcacgcgcg ttggctttcc gcgcgtcggc
      481 ctacggtccg ttccgatggg atcggccggg gacgccgtgg ccacccgctt aaagccgcgt
      541 ccggccgcca gcctgacggc ctcgaaacca tcagagccta tggctccggc ccagagctcc
      601 gcccacagcc gctcctccag ctccgctatc gagaggccgt gcctggcctt gatggcccag
      661 gcgtcgagcg ggccatcgcg cggcgagagg agggcgctcg gcgttcccgg cgcgaagacc
      721 tcgtagtcgg cggcggccgc gaacgctacg acgcgctccc cggcgccgaa ccagatgaac
      781 tcaccttcgg cgaggagccg atccaggtac tcgggacggt agcccggtac gcgggccggc
      841 aggatctcgg tctcccagag ccccgccggc gccgggtagc ccgaaagcgt ctcgagcgca
      901 cgcctcgtgg ccgctaggcc ggccatggca tcgtccgggg ccggctccgc gtcgggcagt
      961 aagcggtcgc gcaggccctg cagggccgcg acgaaggcgg gcaggtctcg ggcgggcctt
     1021 gcccgtacct tgggcctggt ggcggcgcgg ccctgcctga ggagcgattc gagcacgtcg
     1081 cggtcgcagg cgcccggggc gtcggcggcg tgagcgctga ccgagggagc ttcgaccgcc
     1141 ggctcgccgg atcgcgagcg gggctggacg ccgaggccgg ggccgacgac gacgccggcc
     1201 gcctcgagga gcccgggctc ggccgcgccc ggcccgaaca ggcagccgag ccggccgagc
     1261 gttacggggc cggcgtaggg cagccagcgc gccgcgagcg cggcctggcc gtcgggccgg
     1321 ctcgcggcct cgatcagggc ggccttgccc tcgcgcctcg tcacgacggc ttcctgggcg
     1381 ccgggcagcg tgaaggcttc gagcctggat aggagcccgg ttccggcgcc cgggctggcg
     1441 agcgccgcta gcgcggcttc gcgaagctcc tcggggcacc acgcgaggag ggcgtcccat
     1501 tcgtcgagcg gtat
//
LOCUS       DAOPHV010000672         1505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_122325_length_1505_cov_3.911724, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000672 DAOPHV010000000
VERSION     DAOPHV010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122325_length_1505_cov_3.911724"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLQ29_13230"
     CDS             complement(<1..488)
                     /locus_tag="PLQ29_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG87655.1"
                     /translation="METTANGAPLVEMRGICKSFPGVQASDGVDLRLDSGEVLALLGE
                     NGAGKSTLMNILSGLYRQDSGDVYLRGELVDIRSPRDAAALGVGMVHQNFMLVDTMTV
                     AENVILGMAGLPFVPDMVAVKRSIVELGERYNMAVDPDAYIWQLSVGEQQRVEILKQL
                     YR"
     gene            complement(572..>1505)
                     /locus_tag="PLQ29_13235"
     CDS             complement(572..>1505)
                     /locus_tag="PLQ29_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPG87656.1"
                     /translation="DVARLELEAALGDKVKTAYMENVPEGPDATRVVRQYAQDGYDMI
                     FATSFGYMDPMAEVAAEFPDVKFVHCSGYKTSDNMSVYFGRIYQARYLAGIVAGRMTK
                     SNIIGYPGAFPIPEVVRGINAFTLGARSVNPKAEVRVVWTNTWYDPVKEREAAVALLD
                     AGADIITQHQDTVEPQKAAQERGKLSIGYDSDMRKFVGDTVLTSPVWNWAPFYIDTVK
                     QGIAGTWKTGSYWEGLSADVVRLAEFSPLVPQEIRDEVSAVKQKIVGGAWDVFSGPIK
                     DNTGKLRVSEGAKMSDEELLSFDWFVEGVVGKVQ"
BASE COUNT          242 a          485 c          494 g          284 t
ORIGIN      
        1 ttgcggtaca gctgcttgag gatctcgacg cgttgctgct cgccgaccga cagctgccaa
       61 atgtaggcgt ccgggtcgac ggccatgttg tagcgctccc cgagctcgac gatggagcgc
      121 ttgaccgcga ccatgtccgg cacgaacggt aggcccgcca tgccgaggat gacgttctcg
      181 gccacggtca tcgtatcgac cagcataaaa ttctggtgga ccatgccgac gccgagcgcg
      241 gccgcgtccc ggggcgagcg tatgtcgacc agctcgccgc gcaggtacac gtccccggag
      301 tcctgtcggt acaggccgga cagtatgttc atcagggtgc tcttgcctgc cccgttctcg
      361 ccgagcaggg cgagcacctc gccgctatcc agcctgaggt cgacgccgtc cgacgcctgt
      421 accccgggga agctcttgca tatcccgcgc atctcgacga gcggagcccc gtttgcggta
      481 gtttccatcg ttgctgacac cttgtacgtg aatgatttga agtaggaaaa aaagggcgtc
      541 ccgggaggga cgcccctcgc tctctttgga cttactgaac cttgcctacc acgccctcga
      601 cgaaccagtc gaacgacagg agctcctcgt cggacatctt ggcgccttcg gagacgcgca
      661 gcttgccggt gttgtccttg atggggccgg agaacacgtc ccaggcgccg ccgactatct
      721 tctgcttgac agccgagacc tcgtcgcgga tctcctgcgg cacgagcggg ctgaactccg
      781 cgaggcgcac cacgtcggcc gacaggccct cccagtacga gccggtcttc caggtgccgg
      841 ctatgccctg cttgaccgtg tcgatataga acggggccca gttccatacg gggctcgtca
      901 gcacggtgtc gccgacgaac ttgcgcatgt cggagtcgta gccgatggat agcttgccgc
      961 gctcctgggc cgccttctgc ggctcgacgg tgtcctggtg ctgggtgatg atgtcggcgc
     1021 ccgcgtcgag cagggccacg gcggcctcgc gctccttgac cgggtcgtac caggtgttcg
     1081 tccagacgac gcggacctcg gccttcgggt tgaccgagcg cgcgccgagc gtgaacgcgt
     1141 tgatgccgcg gacgacctcg gggatcggga aggcgcccgg gtagccgatg atgttagact
     1201 tggtcatgcg gccggcgacg atgccggcga ggtagcgggc ctggtatatc ctgccgaaat
     1261 agacggacat gttatcggag gtcttgtatc ccgagcagtg cacgaacttt acgtccggga
     1321 actcggccgc gacctcggcc atcgggtcca tgtagccgaa cgacgtggcg aagatcatgt
     1381 cgtagccgtc ctgggcgtac tgtcggacga cgcgcgtggc gtccggcccc tcgggcacgt
     1441 tctccatata ggccgtcttg accttgtcgc cgagggcggc ctcgagctcg aggcgggcca
     1501 cgtcg
//
LOCUS       DAOPHV010000673         1503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_122653_length_1503_cov_2.264503, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000673 DAOPHV010000000
VERSION     DAOPHV010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122653_length_1503_cov_2.264503"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1355
                     /locus_tag="PLQ29_13240"
     CDS             183..1355
                     /locus_tag="PLQ29_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HPG87657.1"
                     /translation="MKEEYEFPLEDYMSRERFERMKRFSQDKATPCLILDLDVVREKY
                     EELRRNMPYAKVYYAVKANPNDEVVALLRDLGSCFDVATRFELDQLLRLGVTPDRMSF
                     GNTIKKERDIAYFYEKGVRLYTTDSFEDVDKIARAAPESKVFFRILTEGLGADWPLSR
                     KFGSHPEMTRNLILHARDLGLEPYGISFHVGSQQRDIGQWSTALGNVGNIYSWAEDQG
                     VKLRMINMGGGFPAMYTDPTNPIEHYAGDIRSFLTNNFGDELPEIIIEPGRSMTGDCG
                     MIVTEIVNIAKKSTHDLYHWVFFDIGKFGGLIETLDEAIKYPIYFEKSGPTLDIILAG
                     PTCDSMDILYEKTPYEMPAGTKIGERAYILTTGAYTSSYSSIYFNGFPPLATYVMK"
BASE COUNT          328 a          460 c          445 g          270 t
ORIGIN      
        1 tgctggctgg tggctatgga catgatcagc tcgaataata cggtatggcc tatctttggt
       61 caatcaaagg ggatggatgg accggctata aacgatattg acaaaggggg tggctgaacc
      121 tatcttttgc gcatgcggcg cccggggcgc ggccatcaaa ccgctaggtt aaggtaggat
      181 cgatgaaaga agagtacgag ttcccgctcg aggactacat gagccgggag cgcttcgagc
      241 ggatgaagcg gttctcccag gacaaggcga cgccgtgcct gatcctggat cttgacgtcg
      301 tacgggagaa gtacgaggag ctccgccgga acatgccgta cgccaaggtc tattacgcgg
      361 tcaaggctaa cccgaacgac gaggtagtcg ccttgctgcg ggatctcggc tcctgcttcg
      421 acgtggccac gcgcttcgag ctggatcagc tgctcaggct cggcgtgacg ccggaccgca
      481 tgagcttcgg gaacacgatc aagaaagagc gcgacatcgc ctatttctac gaaaagggcg
      541 tcaggctcta caccaccgat agcttcgagg acgtggacaa gatagcccgc gccgcgccgg
      601 agtccaaggt gttcttcagg atcctcaccg agggcctcgg agccgattgg cccctgtcga
      661 ggaaattcgg ttcccacccc gagatgacgc gcaacctgat actccatgcc cgcgacctcg
      721 ggctggagcc ctacggcatc tcgttccacg taggcagcca gcagcgcgac atcggccagt
      781 ggtcgacggc gctcggcaac gtaggcaaca tctactcctg ggccgaggat cagggcgtca
      841 agctgcgcat gatcaacatg ggcggcggct tcccggccat gtataccgat ccgaccaacc
      901 ccatcgagca ttacgcgggc gacatcaggt ccttcctgac caacaacttc ggcgacgagc
      961 tacccgagat catcatcgaa cccggtcggt cgatgaccgg cgactgcggt atgatcgtca
     1021 ccgagatcgt caacatcgcc aagaagagca ctcacgacct gtaccattgg gtcttcttcg
     1081 acataggtaa attcggcggg ctcatcgaga ccctggacga ggcgatcaag tacccgatct
     1141 acttcgagaa gtctgggccg accctcgaca ttatactcgc cggccccacc tgcgacagca
     1201 tggacatact ctacgagaag accccgtacg agatgcccgc cggcaccaag attggcgagc
     1261 gcgcctacat cctgacgacc ggcgcctaca cctcgagcta tagctcgatc tacttcaacg
     1321 gcttcccgcc gctggccacc tacgtgatga aataaaacga tcggaggccg tcacggctat
     1381 gcgagcggcg acggcctcca gtattcaggc ttcccgaggc tcttcgcctc gcgaagcgcg
     1441 cctcgtcagg agcgcgtcat cgaggcggcc gtgcggggta tgttgcccaa gaacaagctg
     1501 ggc
//
LOCUS       DAOPHV010000674         1494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_123744_length_1494_cov_3.585823, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000674 DAOPHV010000000
VERSION     DAOPHV010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123744_length_1494_cov_3.585823"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="PLQ29_13245"
     CDS             complement(<1..1258)
                     /locus_tag="PLQ29_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87658.1"
                     /translation="MEDRARDEIRDDILERVFPELRFGEDPDIERYFELRASGRMLDA
                     LAVYRSRLRPRYPDDAQRVVLLKLYRIKSPAFPEYLRGLMRARADDIIARLRANIDAL
                     LEPLSGIKLGKTYAVLKAVERMARMLPDDPERARQAAWAYADYARILGYRRAEAERAS
                     YLLGQFYDQATEDEGADFIAASVATEERRLKERREESEKNFFDLSKIEFDEEDVRRIE
                     IPAGLERDEDLVLAWCHKYWLRVDDPAFERIVWLYSKKYGTKHYEVFKAIKTGRHRKY
                     QDDDILTMVATTIATRYSYTVQGDLYMQSAWRRVKASMYGQVPPRRPAQTETTEREPA
                     QRKPRSAKPRRERKAATPLVTQTRPKPDERPKLRPAAAPAKAIAIPDDRRPTGSISDR
                     IRRLSGRAYDVYRDIFLAKVRVSIREE"
     gene            complement(1370..>1494)
                     /locus_tag="PLQ29_13250"
     CDS             complement(1370..>1494)
                     /locus_tag="PLQ29_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPG87659.1"
                     /translation="DADMASEMVEFTKNQILAQAGNAMLAQANQKTQQVLQLLQ"
BASE COUNT          209 a          452 c          507 g          326 t
ORIGIN      
        1 gttcctcgcg tatgcttacg cgtaccttgg cgaggaaaat atcccgatat acgtcatagg
       61 cgcgaccaga aagccgcctg atcctgtcgc tgatggagcc cgtcggcctt cggtcgtccg
      121 gtatggctat ggccttggcg ggagcggccg ccggccgaag tttcggccgc tcgtcgggct
      181 tcggtcgggt ctgggtgacg aggggcgtcg ccgccttacg ctctcgcctg ggctttgccg
      241 accgcggctt acgctgggcg ggctctctct cggtggtttc tgtttgcgcc ggcctacgcg
      301 gcgggacctg tccgtacatg gacgccttga cgcgacgcca tgctgactgc atgtacaggt
      361 cgccttgaac ggtgtaactg tagcgggttg cgatggtcgt ggcgaccatg gtcagtatat
      421 cgtcgtcttg gtacttccgg tgacggccgg tcttgatggc tttgaacacc tcgtagtgct
      481 tcgtgccata tttcttggag tagagccata cgatgcgctc gaaggccggg tcgtcgaccc
      541 tgagccaata cttgtggcac caggcgagca cgaggtcctc gtcgcgttcg aggccggccg
      601 ggatctcgat gcgccgaacg tcctcttcgt cgaattctat cttggatagg tcgaagaagt
      661 tcttctcgct ctcctcgcgt cgctctttga gcctgcgttc ctcggtggcg acgctcgcgg
      721 ctatgaagtc ggcgccttcg tcctcggtcg cctggtcgta gaactgtccg agtaggtagg
      781 acgctcgctc ggcttcggcg cgccggtagc ccagtatcct cgcgtagtcg gcgtaggccc
      841 aggccgcctg cctggcccgc tccgggtcgt cgggtagcat cctggccatg cgctcgacgg
      901 ccttgagcac cgcgtaggtt ttgcccagct tgatgccgga caggggctcg agtagcgcgt
      961 cgatgttggc gcgtaggcgg gcgatgatat cgtcggcccg cgcgcgcatg aggccgcgta
     1021 gatactcggg gaaggccggg gacttgatcc tgtacagctt gaggaggacg acgcgctggg
     1081 cgtcgtccgg gtagcggggg cggaggcggc ttcggtagac ggcgagcgcg tcaagcatgc
     1141 gccccgacgc tcgtagctcg aagtagcgct ctatgtccgg atcctcgccg aagcgtaatt
     1201 ctgggaagac gcgctcgagt atatcgtcgc gaatctcgtc ccgggctcta tcttccatct
     1261 ggcggcatta tacgcggctc tcggacgctc ttcaagcgag cggtgctggt ccgaaaaaaa
     1321 agggccgccc ccggaagggc ggccctcgtc acgtccgcgc ggccacggcc tactggagga
     1381 gctgcagtac ctgctgggtc ttctggttgg cctgggcgag catcgcgttg ccggcctgcg
     1441 cgaggatctg gttcttggtg aactcgacca tctcgctggc catgtccgcg tcgc
//
LOCUS       DAOPHV010000675         1494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_123748_length_1494_cov_3.480195, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000675 DAOPHV010000000
VERSION     DAOPHV010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123748_length_1494_cov_3.480195"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1494)
                     /gene="secA"
                     /locus_tag="PLQ29_13255"
     CDS             complement(<1..>1494)
                     /gene="secA"
                     /locus_tag="PLQ29_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695026.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPG87660.1"
                     /translation="GRRYSDGLHEAIEAKERIKIARRNRTLATITFQNYFRMYDKIAG
                     MTGTAETESVEFQKIYELDVVVIPTNKPVARLDEDDLVFLNESEKFEAICDEIAEAHA
                     KGQPMLVGTVSIDKSERLSAALTRRGVRHEVLNAKNHAREATIIAEAGAKGAVTIATN
                     MAGRGTDIKLGGNPEYRARKKVGTSADERTYREALEKEYELWRADYEEVKAAGGLYVL
                     GTERHESRRIDNQLRGRSGRQGDPGRSRFFISLDDDLMRLFGGSNLKGLMMKIGMKEG
                     EPIYHPLLNKTMANAQKRVEERNFDIRKHLLEYDDVLNQQRKFIYDQRDAIVKDDDLP
                     ARVVGTAREMLDALLERYEDGLKADQKAAYAELQDALKASFGFVLPFRSDAPESRKLD
                     DLRATATGELDRDLEDKLAMAGSDNLNHFLRWKYLSAIDQKWLDHLENMEALREAVYL
                     RHYAQKNPLLEYKLEGFELFDQMIEDIRKSVASTLYLVRIQRQEDRA"
BASE COUNT          201 a          457 c          503 g          333 t
ORIGIN      
        1 cggcccggtc ctcctgcctc tggatgcgca cgaggtacag cgtgctcgcg acgctcttgc
       61 ggatgtcctc gatcatctgg tcgaacagct cgaagccttc cagcttgtac tcgagcaggg
      121 ggttcttctg cgcgtagtgc ctgaggtaca ccgcctcgcg gagcgcttcc atgttctcga
      181 ggtggtcaag ccacttctgg tctatagccg acaggtactt ccagcgcagg aagtggttca
      241 ggttgtccga gccggccatc gccagcttgt cctcgaggtc gcggtccagc tcgcctgtcg
      301 cggtggcgcg aaggtcgtcg agcttcctgg attcgggggc gtccgagcgg aacggcagga
      361 cgaagccgaa cgaggcttta agggcgtcct ggagctcggc gtaggcggcc ttctggtcgg
      421 ccttgagtcc gtcctcgtag cgctcgagca gggcgtcgag catctcccgg gccgtgccga
      481 cgactctggc tggcaggtcg tcgtctttta cgatggcatc gcgctgatcg tagatgaact
      541 tacgctgctg gttgagcacg tcgtcgtact cgaggaggtg cttgcggatg tcgaagttgc
      601 gctcctcgac gcgcttctgg gcgttggcca tcgtcttgtt gaggagcgga tggtagatcg
      661 gctcgccttc cttcatgcct atcttcatca tcaggccctt gaggttggat ccgccgaaca
      721 ggcgcatcag gtcgtcgtcg agcgagatga agaagcgcga tcggccggga tcgccctgac
      781 ggccggagcg gccgcgtagc tggttatcga tgcgcctcga ctcgtggcgc tcggttccga
      841 gcacgtacag gcctccggcg gccttgacct cctcgtagtc ggctcgccat agctcgtact
      901 ccttctcgag cgcctcgcgg taggtccgtt cgtcggccga ggtgccgacc ttcttgcgcg
      961 cgcggtactc ggggttgccg ccgagcttga tgtcggtacc gcggccggcc atgttcgtcg
     1021 ctatggtcac ggcgcccttg gcgccggcct cggcaataat cgtagcctcg cgggcgtggt
     1081 tcttggcgtt gaggacctcg tggcgcacgc cgcgcctggt gagggcggcg gagaggcgct
     1141 cggacttgtc gatgctgacc gtgccgacga gcatcggctg gcccttggcg tgggcctcgg
     1201 ctatctcgtc gcagatggcc tcgaatttct ccgactcgtt caggaacacg aggtcgtcct
     1261 cgtcgagcct ggcgaccggc ttgttcgtcg ggatcacgac gacgtccagc tcgtatatct
     1321 tctggaactc gaccgactcg gtctcggccg tacccgtcat gcccgctatc ttgtcgtaca
     1381 tgcggaagta gttctggaag gtgatcgtgg cgagggtgcg gttgcgcctg gctatcttga
     1441 tgcgctcctt ggcctcgata gcctcgtgga gcccgtcgga gtatcggcgg ccgt
//
LOCUS       DAOPHV010000676         1489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_124414_length_1489_cov_4.582287, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000676 DAOPHV010000000
VERSION     DAOPHV010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124414_length_1489_cov_4.582287"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PLQ29_13260"
     CDS             complement(<1..363)
                     /locus_tag="PLQ29_13260"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HPG87661.1"
                     /translation="MRGEPAFDRLAVVLCRVEGSMNVGAACRAMKAMSVRRLVLAACP
                     DHDQTEVRTHALGAYDLYEAAERFDDLGEALAGFSLAAGLTRRVGQRRKESAALPDFA
                     ARLAARLVAAEGRAGQASS"
     gene            429..1187
                     /locus_tag="PLQ29_13265"
     CDS             429..1187
                     /locus_tag="PLQ29_13265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPG87662.1"
                     /translation="MTNKSDERGSTGLDGRTALVIGATGGIGAAIAGALAEGGAVLTI
                     HGRDDSRLAAKASELAKYGNEVGTIRADLSEGRAPPELLRAAASIDLLVVAYGPFVYK
                     PLAETSQADWSLAALGCLALPGTIATEAAVGMAGRGFGRILLFGGTRTDAVRGFKANA
                     AYAAAKTGLGVVAKSIAVEFGGRGVSCAVVCPGFVDTEYLDEAMRSRLEKASPRGGLI
                     PAAELASLVAYLLGGGMDLANGSVVVADRGLYAL"
     gene            1368..>1489
                     /locus_tag="PLQ29_13270"
     CDS             1368..>1489
                     /locus_tag="PLQ29_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HPG87663.1"
                     /translation="MPNNDIVPGFDDEKDDSLKIRLQKIDQNCLALFLTGYIDT"
BASE COUNT          274 a          476 c          472 g          267 t
ORIGIN      
        1 tgagcttgcc tgtccggccc gcccctcggc ggcgacgagg cgcgccgcga gtctggccgc
       61 gaaatcgggt agcgccgcac tctccttacg ccgctgtccg acccgcctcg tcaagccggc
      121 ggccagcgag aaaccggcga gggcctcgcc taaatcgtcg aatcgctcgg ccgcctcgta
      181 gaggtcatag gctccgagcg cgtgcgttcg cacctccgtc tgatcgtgat cggggcaggc
      241 cgccagcact agtcggcgca cgctcatcgc cttcatcgca cggcatgccg cccctacgtt
      301 catcgagcct tcgacgcggc atagcaccac cgccagccgg tcgaacgccg gttctcccct
      361 catcgggcct ccatcgagcc tccccgttgt ttacacgggg cgccgatcgg actatagttc
      421 aagctctgat gacgaacaag agcgacgagc ggggctcgac cggcctcgac ggaaggaccg
      481 cgctggtgat cggcgcgacg ggcggcatag gggcggccat tgccggcgca ctggccgagg
      541 ggggcgccgt cctgaccatc cacgggcgcg acgacagccg cctggcggcc aaggcttccg
      601 aactggccaa atatgggaac gaggtcggta ccatcagggc cgacctatcc gagggccggg
      661 ctcctcccga gctgctgagg gccgccgcct cgatcgacct cctcgtcgtc gcctacgggc
      721 ccttcgtcta taagcccctc gcagagacca gccaggctga ttggagcctc gccgcgctcg
      781 ggtgcctggc cttgccgggg acgatagcga ccgaggccgc cgtcgggatg gccgggcgcg
      841 gcttcggtcg tatcctcctc ttcggcggca cgaggacgga tgccgtacgt ggcttcaagg
      901 ctaatgccgc ctatgcggcc gccaagaccg gcctgggcgt cgtggccaag tcgatagccg
      961 tggagtttgg cggccgcggc gtctcgtgcg ccgtcgtatg tccggggttc gtggataccg
     1021 aataccttga cgaagcgatg cgttcgaggc tcgagaaggc ctccccacgg ggcggactga
     1081 tcccggccgc ggagctggcc tctttggtag cgtatctatt aggtggcggc atggatctcg
     1141 cgaacggttc ggtcgtcgtc gccgatagag gcctatacgc tctgtagcaa gcgcgatata
     1201 taatagagct tacgtggacg acttaaattg ccgaaaataa cgtcgaaggg atcagtcgat
     1261 cggtcgggcc gtcagtatcg atttgacaag ccattgacga cggcctaaga tggtcatagt
     1321 attgaccttc agtaaaacaa tagacctcac gatggaagag gagcaccatg ccaaataacg
     1381 atatcgttcc tggcttcgac gacgagaagg acgatagcct taaaattcgg ctccagaaaa
     1441 tcgaccagaa ctgcctggcg ctgttcttaa ccggctatat cgatacata
//
LOCUS       DAOPHV010000677         1489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_124425_length_1489_cov_3.937238, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000677 DAOPHV010000000
VERSION     DAOPHV010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124425_length_1489_cov_3.937238"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..947)
                     /locus_tag="PLQ29_13275"
     CDS             complement(117..947)
                     /locus_tag="PLQ29_13275"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPG87664.1"
                     /translation="MNGSLYLVPVPIGDGDPADELAPRVLRIAAGIRDFVVENERSAR
                     RFLCRVMAQDALDASRFGVLDEHTRPEDVRALLAPLLAGRDVAILSEAGSPCVADPGA
                     ALVAEAAEAGIRVVPLPGPSSILMAVMASGLGGQRFAFAGYLPPDQAGREAALRALEA
                     DSRRQGSTQAFIETPYRNDAMVASAACALAPDTVFCAAIGLSTPEERIVRMTAAAWKA
                     GPVRIGKKPTVFLLKAADGNRGGASGRDRRGGSPFDRKAQPDRKAPPDRKAPPRKRGS
                     "
     gene            complement(944..>1489)
                     /locus_tag="PLQ29_13280"
     CDS             complement(944..>1489)
                     /locus_tag="PLQ29_13280"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPG87665.1"
                     /translation="TATVRNLATLGGNVASRDSFMTCFPSLACMDATVELRDASGSRW
                     TGIHSLVDGSGRPAFPAATLLTRIRIPTAPWDAFALHRLGEPTKGDAFASTFAAAARF
                     EKETISELRLIASGRTLARDRALELSLIGKRLPLTVKDVEEAQEAAREQAIEAGFPAR
                     LAERFGAYVMSFLANVQEGGR"
BASE COUNT          223 a          509 c          516 g          241 t
ORIGIN      
        1 aggcccatgc gggcctgggc gaacggcgtt atcgcggcgg tttcgttctg gtattgggct
       61 aataggtccg tttgatgatg catggctatg ccagtgtaac cttcaatgcg gaatcgtcaa
      121 gatccgcgtt tgcggggcgg cgcctttcga tcgggcggcg cctttcgatc gggctgcgcc
      181 tttcgatcga aaggcgatcc tccgcgcctg tcccggccgc tggccccgcc ccggttgccg
      241 tcggcggcct tcagcaggaa taccgtcggc ttcttgccta tgcgcacggg cccggctttc
      301 caggcggcgg cagtcatgcg aacgatgcgc tcctccggcg tcgataggcc gatcgcggcg
      361 cagaacacgg tatcgggagc cagtgcgcag gccgcgctgg cgaccatggc gtcgttgcgg
      421 tatggcgtct cgatgaaggc ctgcgtcgaa ccctgccgcc tcgagtcggc ctcgagcgcg
      481 cgcagggcgg cctccctgcc ggcctggtct ggcggtagat agccggcgaa cgcgaagcgc
      541 tggcccccga ggcccgaggc catgacggcc atcaggatcg agctcgggcc gggcaggggg
      601 acgactctga tccccgcctc ggctgcctcc gccaccaggg ccgctcccgg gtcggcgacg
      661 caggggctac cggcctccga cagtatggcc acgtcccgcc cggccagcag gggcgccagc
      721 agcgcccgca catcctcggg ccgcgtatgc tcgtccagca cgccgaagcg cgacgcatcg
      781 agggcgtcct gcgccatgac gcggcatagg aagcgcctgg cgctgcgctc gttctcgacg
      841 acgaagtcgc gaatgccggc cgctatcctg agtaccctcg gggccagttc gtccgccggg
      901 tcgccgtcgc ctatcggcac cggcaccaaa tatagtgagc cgttcatctc ccgccttcct
      961 ggacgttggc caggaacgac atgacgtacg cgccgaagcg ttcggccagc ctggccggga
     1021 aaccagcctc gatcgcctgt tcgcgcgcgg cctcctgggc ctcctcgacg tcctttacgg
     1081 tcaacggcaa cctctttcct atcagcgaaa gctcgagggc gcggtcgcgc gccagcgtcc
     1141 tccccgacgc tatgagccgc agctccgata tggtctcttt ctcgaaacgg gcggcggcgg
     1201 cgaaggtcga cgcgaaggcg tcccccttcg tcggctcgcc gagccggtgg agcgcgaacg
     1261 cgtcccaggg ggcggtagga atgcgtatcc gggtcagcag ggtagcggcc gggaaggccg
     1321 gtcgcccgga accgtcgacg agggagtgga tgccggtcca gcgcgagccc gaggcgtcgc
     1381 ggagctccac ggtcgcgtcc atacaggcca gggacggaaa acaggtcatg aacgagtcgc
     1441 gcgaggcgac gttaccgccg agcgtggcca gattgcgtac tgtagccgt
//
LOCUS       DAOPHV010000678         1488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_124588_length_1488_cov_3.302861, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000678 DAOPHV010000000
VERSION     DAOPHV010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124588_length_1488_cov_3.302861"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="PLQ29_13285"
     CDS             <1..893
                     /locus_tag="PLQ29_13285"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPG87666.1"
                     /translation="ISRVKTDGPNSLYLWLPFPADTPSQRGVSALARSAEPLLPDYRG
                     LSAYRLVDLAPDKLATVGHDHLVQVYGVDTDVNPDRIKPPPSPAPRLYETLVRPDALV
                     PADDPSVVALAKKAAGREKNHYRIALATLQALNQAVRYDSGAVSESPVKALEGKVADA
                     WDLAVLYAAALRASGVPALPVAGVVVDDTRRAWNHAWAEFYIYGFGWVPVDPVLASGG
                     DVGRFSPSFEDRSRYFGDMDDRHIAFSRGLAIVDRVTPDGRTVSAARRYSFQNIFEEA
                     AGGLKAYTSFWSDIEITGVY"
     gene            1147..>1488
                     /locus_tag="PLQ29_13290"
     CDS             1147..>1488
                     /locus_tag="PLQ29_13290"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525450.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPG87667.1"
                     /translation="MSYIQNEDSELYSAIQGEQDRQRKNLELIASENYASKAVMEATG
                     SVLTNKYAEGYPGKRYYGGCEYVDVAEDLARDRAKALFGADYANVQPHSGSQANMGVY
                     FAALKPGDTILG"
BASE COUNT          255 a          494 c          467 g          272 t
ORIGIN      
        1 ccatatcgag ggtcaagacc gacgggccca actcgctgta cctctggcta ccgttcccgg
       61 ccgatacgcc gtcgcagcgc ggagtgtcgg cgctcgcgcg cagcgccgag ccgctcctgc
      121 ccgactatcg cggcttgtcg gcctacaggc tcgtcgacct ggcgccggac aagctggcga
      181 ccgtcggcca tgatcacctg gtccaggtct acggcgtgga taccgacgtg aaccctgacc
      241 gcatcaagcc gccgccgtcg ccggccccga ggctgtacga gacgctggtc cggcccgacg
      301 cgctcgtgcc ggccgacgat ccctccgtcg tcgccctggc caagaaggca gccggccgcg
      361 agaagaacca ttaccgcatc gcgctcgcga ccctgcaggc gctcaaccag gcggtccgct
      421 acgattccgg agcggtgtcc gagtctccgg tcaaggcgct cgagggcaag gtcgccgatg
      481 cctgggacct ggccgtgctc tacgccgcgg cgctcagggc ctcgggcgtt ccggccctgc
      541 cggtagctgg cgtcgtggtg gacgataccc ggcgcgcctg gaaccatgct tgggccgagt
      601 tctacatcta cggcttcggt tgggtgccag tggacccggt gctcgcgtct ggcggagacg
      661 tcggccggtt ctcgccctcg ttcgaggata gatcccgcta cttcggcgac atggacgata
      721 ggcatatcgc gttctcccgg ggcctggcca tcgtcgatcg cgtcacgccc gacggccgca
      781 cggtctcggc cgccaggcgc tattcgttcc agaacatctt cgaggaggcc gcgggcggcc
      841 tcaaggccta tacctcgttc tggagcgaca tagagatcac cggcgtgtat taggccgatg
      901 gggaaatgaa ccacggaggc acggagacgc ggaggaaatg cgataggact ggatagttag
      961 gataaggggg agccgctaaa ggcctccttg ccccgctcat ccgacgcgta tagcggaatc
     1021 ctgccctttg ctctttctcc tcttctccgt gcctccgtgt ctctgtggct agattcatcg
     1081 ctaccgctcg ctttttaccc gctcggttag tatggtatgc tgtagcgtct tctaaggagg
     1141 tatctcatgt cgtatatcca gaacgaggat tcggaactct attcagccat acagggagag
     1201 caggataggc agcgcaagaa tctcgagctc atcgccagcg agaactacgc gtccaaggcc
     1261 gtcatggagg ctaccggctc ggtgctgacc aataagtacg ccgagggcta tccaggcaag
     1321 cgctactacg gtggctgcga atacgtcgac gtggccgagg acctcgcgcg cgaccgcgcc
     1381 aaggccctgt tcggcgccga ctacgccaac gttcagcccc attcgggctc gcaagccaac
     1441 atgggcgttt acttcgcggc gctcaagcca ggcgacacca tcctcggc
//
LOCUS       DAOPHV010000679         1486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_124804_length_1486_cov_4.013277, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000679 DAOPHV010000000
VERSION     DAOPHV010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124804_length_1486_cov_4.013277"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLQ29_13295"
     CDS             complement(<1..417)
                     /locus_tag="PLQ29_13295"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPG87668.1"
                     /translation="MLAAVQFPSWIRPEILPFLPQFPLRWYGLMYLVAFGVTYLLFKR
                     QVRTRRLGYSDDDISGFFLAGIVGLILGARLFGTLLYDTSGLYWSKPWLIFWPFGEGG
                     KFTGFMGMSFHGGMVGLIVGILIYSKRLRLDFLEWAD"
     gene            complement(542..>1486)
                     /locus_tag="PLQ29_13300"
     CDS             complement(542..>1486)
                     /locus_tag="PLQ29_13300"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPG87669.1"
                     /translation="KDPSVVDPPSFDDSIVEERRENGDRLLKAAKSGELRFEKNALSI
                     EAAQRVKGDVGPATGNIKFPGPVAIAGTVLTGYAVVAGGDVTVTGAVEAALVSADGSV
                     KIGEGVKGAKRGTIRARKGLEASFAEQAMLLAVEDITLKNSALLCNVKTNGLLKLLGE
                     RGHLIGGICRARKGVEVQNLGSANGARTQVSFGQDYLVKDAIEAEEREIERVKALILQ
                     TDKRMRELEADGGSLDKVRQDKLRLVKLLEQRSLRVFELREKFEEHFPSEIVVRGTVF
                     AGVVIESHNRFHEVKQAKQKVAFSFDPQLGRVVERPLK"
BASE COUNT          268 a          500 c          461 g          257 t
ORIGIN      
        1 atcggcccac tcgaggaaat ccagccgcaa gcgcttcgag tagatcagaa tgccgacgat
       61 caggccgacc atgccgccgt ggaacgacat gcccatgaag ccggtaaact tgccgccctc
      121 gccgaagggc cagaagatca gccaaggctt gctccagtac aggccgctcg tatcgtagag
      181 cagggtgccg aacagcctgg ccccgaggat cagcccgacg atgccggcca gaaagaaacc
      241 ggagatatcg tcgtcggaat agccgaggcg cctcgtcctg acctgacgct tgaacagcag
      301 gtaggtgacg ccgaaggcga cgaggtacat gagcccgtac cagcgcagcg ggaactgcgg
      361 caggaacggc agtatctcgg gtcggatcca cgacggaaac tgaactgcgg cgagcatcgg
      421 tacctccacg gtctgcttgg cttgctggta ctatacccgg gcgggcggcc cgggcgcaac
      481 ggccgcgacg ccgctagcgc ggcgacgcta ctgcggcgac gacgctactg cggcgacgac
      541 gctacttgag cggccgctcg acgacgcggc cgagctgggg gtcaaaggaa aaggccacct
      601 tctgcttggc ctgcttgacc tcgtgaaagc ggttatggct ctcgatgacc acccccgcga
      661 acaccgtgcc ccggacgacg atctcgctcg ggaagtgctc ctcgaatttt tccctgagct
      721 cgaagacccg gagcgatcgc tgttcgagca acttgacgag ccggagctta tcctggcgga
      781 ccttatcgag gctcccgccg tcggcctcga gctcgcgcat ccgcttatcg gtctggagaa
      841 tcagcgcctt gactctctcg atctcccgct cctccgcctc gatcgcgtcc ttgacgagat
      901 agtcctggcc aaaggacacc tgggtgcgcg caccgttggc cgagccgagg ttctgcacct
      961 cgactccctt cctggcccgg cagatcccgc cgatcaggtg gccgcgctcg ccgagcaact
     1021 tgaggaggcc gttggtcttg acgttacaga ggagggcgga atttttaagc gtaatgtcct
     1081 cgacggccag gagcatggcc tgctcggcga acgacgcctc gaggcccttg cgcgctcgta
     1141 tcgttccgcg cttggcgccc ttgacgccct cgcctatttt aaccgagcca tcggcggaga
     1201 cgagggccgc ctctacggcg ccagtcaccg tgacatcgcc cccggccacc acggcatagc
     1261 cggtcagcac cgtaccggct atggccaccg ggccggggaa cttgatgttg ccggtggccg
     1321 gcccgacgtc gcccttgaca cgctgcgccg cctcgatgga cagggcgttc ttctcgaagc
     1381 gcagctcgcc gctcttagcg gccttgagca gtcgatcgcc gttctccctt cgctcctcga
     1441 ctatcgaatc gtcgaagctc gggggatcga caacgctcgg gtcctt
//
LOCUS       DAOPHV010000680         1486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_124807_length_1486_cov_3.922432, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000680 DAOPHV010000000
VERSION     DAOPHV010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124807_length_1486_cov_3.922432"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLQ29_13305"
     CDS             complement(<1..350)
                     /locus_tag="PLQ29_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87670.1"
                     /translation="MKKKYSLDRVLPGSRFTADAFSDGNNLFVPAGVKVRAKDLEGLK
                     RWGITAVFSEGQLESPDEKPVGQDSSSGAGTGHDDGRAPASKGLAASFSDHALYASYT
                     DMIDRLKALMDKIR"
     gene            complement(347..604)
                     /locus_tag="PLQ29_13310"
     CDS             complement(347..604)
                     /locus_tag="PLQ29_13310"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HPG87671.1"
                     /translation="MRRRSGKKTVALRYDETLPAPIVLAKGSGRVAERIEAIATRTGV
                     PLVRDDVVAAALEPVDIGEFVPPEYWELVARVFVFIRKVRS"
     gene            complement(597..812)
                     /locus_tag="PLQ29_13315"
     CDS             complement(597..812)
                     /locus_tag="PLQ29_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87672.1"
                     /translation="MAADIRCGDSRRLLELRGPRSRPDIAYYSDDQRELSTFELEFGG
                     KGTVRVEGLERGYCGELASGNGLDADA"
BASE COUNT          263 a          507 c          461 g          255 t
ORIGIN      
        1 atacggatct tatccatcag ggccttgagc ctgtcgatca tatccgtata ggacgcgtag
       61 agcgcgtgat ccgagaacga cgccgcgagc cccttcgacg cgggggcgcg cccgtcgtcg
      121 tggccggtcc cggcgccgga cgaggagtcc tggcccacgg gcttctcgtc cggagactcg
      181 agctggccct cgctgaatac cgcggtaata ccccagcgct tgagcccctc gaggtccttg
      241 gccctgacct tgacgccggc gggcacgaat aggttattac cgtccgagaa cgcgtcggcg
      301 gtgaatcgag agcctggcaa gaccctgtcg agcgaatact tcttcttcat gatcgcactt
      361 tccttataaa gacgaacacg cgcgctacga gttcccaata ctccggtggc acgaactcgc
      421 cgatatcgac gggttcgagg gccgcagcca cgacgtcatc gcggaccagc ggcacgcccg
      481 tccgggtcgc tatcgcctcg atgcgctcgg ccactcgccc gctacccttc gctaacacta
      541 tcggcgcagg aagcgtctcg tcatagcgca gggctacggt ttttttaccg gagcgcctac
      601 gcatcggcgt ccaagccgtt cccggaggcc aactcgccgc aatagccgcg ctccagcccc
      661 tcgacgcgca cggtcccctt ccccccgaat tcgagctcga aggtcgacag ttcgcgctgg
      721 tcgtcgctat agtaggcgat atcgggacgc gaacgcgggc cgcgaagctc caggagccgc
      781 cgcgaatctc cgcaacggat atcagcggcc attcttcccg ctactcgagc tcccccctta
      841 ctataatacc aaattcggat aaaaccccga aactccgttc ctccgtactc cagctcgaac
      901 ggcgcgcaga cccaaccgga gccgtcggcc tcaggcttgt gcatggcggc gagcctcgga
      961 tcggacatgg ctcgcccggc cgccttcatg agggcctcgg atagaatagc gacagaaatg
     1021 gcctcgtcct ctgatgtcga ccgtcgagcc gactctcccc cggttcggcg gtcggagttt
     1081 ccggaatttc cggagtcacc ggagccgtcc gaaccgccgc gacggcctcc cggcgagtcg
     1141 aggatacgga gcacgctatc cagatcgggc gactcaggat cgatgccagc ggctaccgcc
     1201 cttgcggcca agcgggcggc ggccggatcg gagccgtggc gcagggcggc gcgacgtacg
     1261 gccatgatcc tgcttggttc tagcctgagt ccctctccca tcagcgcgga tatagccgcc
     1321 gccgacagtg ggtcggcggg aacgcgtagc gaggcgcata gatcggcggt cttggctacc
     1381 cggccatcat gaagagcgct ggaggccggc gctatgggcg gagcgagggt cggtgcggcg
     1441 cgagaccacg gcgacgatgg ttttagcgcc gtcgccccct gaacgg
//
LOCUS       DAOPHV010000681         1484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_125090_length_1484_cov_3.790063, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000681 DAOPHV010000000
VERSION     DAOPHV010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125090_length_1484_cov_3.790063"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..912
                     /locus_tag="PLQ29_13320"
     CDS             46..912
                     /locus_tag="PLQ29_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPG87673.1"
                     /translation="MTDASEISRKPDSGKALLSRSDDRLSIFFIGAGSAFTKRYYQNN
                     ALVVKGDDHLLIDCGTRAPEALTRLGLSVTDIDTYLITHSHADHIGGLEEVMLLNRYM
                     AGKKPTMIAPAAYRAYLWKRSLKGGAAHNERRDGRYLRFEDLWDAVDLEPVPGADRQL
                     CETSVGSIRLAMFRTMHIPDSAPSWRSCAPSYGVVIDGRVLYTSDTRYDPETLRFVEE
                     RYAIEAAFHDCQLFTGGVHASLDELAGLPPALKAKTLLMHYGDAVDSAEAKAETLGFK
                     GFARQWTPYLFG"
     gene            888..>1484
                     /locus_tag="PLQ29_13325"
     CDS             888..>1484
                     /locus_tag="PLQ29_13325"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPG87674.1"
                     /translation="MDAVPLRLGLVAALASALSIVAVGCAGAPPSDDGYADLLMELGA
                     EPRSFMKAGGATVQAFVIGDPGAPKRVVFVHGWSGSGAEFVEMALRLSRARGDVLCIC
                     VDLPGSGSSDKPADASYDVPYFRDVLAEAVEAAATYGLARGQRSTDITLVGHSLGGHL
                     SVAYAASADASIARLALVSPAGWPGEVGAIQAWASKNEF"
BASE COUNT          246 a          507 c          479 g          252 t
ORIGIN      
        1 cgcctcgttt tgaaatcgct ccgaccgtac agtatagtat ggtttatgac cgatgcaagc
       61 gaaatttcgc gcaaaccgga ctcgggcaag gccttgctgt cgaggtcgga cgaccgtcta
      121 tccatattct ttatcggcgc gggtagcgcc ttcaccaagc gctactatca gaacaacgcg
      181 ctcgtggtga agggcgacga ccatctgctc atcgactgcg gcaccagggc gcccgaggcc
      241 ctcacgaggc tcggcctatc ggttaccgat atcgatacct accttataac gcattcccat
      301 gcggaccata taggcgggct cgaggaggtc atgctgctca accgctacat ggccggcaaa
      361 aagcctacga tgatagctcc ggccgcttac cgagcgtacc tctggaagcg ctccctcaag
      421 ggaggcgcgg cccataacga gaggcgcgac ggccgatact tgcgcttcga ggacctctgg
      481 gacgcggtcg acctcgagcc ggtgccgggc gccgaccgtc agctctgcga gacgagcgtg
      541 ggctccatcc ggctggccat gttcaggacc atgcacatcc ccgactcggc gccgagctgg
      601 cgctcctgtg cgcccagcta cggcgtggtc atcgacggtc gcgtgctcta cacgagcgac
      661 acgcgctacg accccgagac gctgcgcttc gtcgaggagc gctacgccat cgaggccgcg
      721 ttccacgact gccagctgtt caccggcggc gtgcacgcct cgctcgacga gctggccggg
      781 ttgccgcccg cgctcaaggc caagaccctg ctgatgcact acggcgacgc cgtcgattcg
      841 gcggaggcca aggccgagac gctcggcttc aagggcttcg cgcgacaatg gacgccgtac
      901 ctcttcggct agggctcgta gcagccctcg cctcggcgct gtcgatcgtc gcggtcggct
      961 gcgccggcgc tccgccctcc gacgatggct acgccgacct ccttatggaa ctcggcgcgg
     1021 agcccaggtc cttcatgaag gctggcggcg cgacggtcca ggccttcgtg atcggagacc
     1081 cgggggcgcc caagcgggtc gtcttcgtgc acggctggtc cggtagcggg gcggagttcg
     1141 tcgaaatggc cctgaggcta agccgggctc gaggagacgt cctttgcatc tgcgtcgacc
     1201 tgccgggttc gggctcctcc gacaagccgg ccgacgcgtc ctacgacgtg ccgtacttca
     1261 gggacgtact agcggaagcg gtcgaggcgg ccgcgaccta cggcctggcc agagggcagc
     1321 gctcgaccga cataactctg gtaggccaca gcctcggcgg ccacctgtcc gtcgcctacg
     1381 ccgcgtcggc ggacgcgagc attgccaggc tcgcgctcgt ctcgccggcc ggctggcctg
     1441 gcgaggtcgg cgccatccag gcctgggcct ccaagaacga attc
//
LOCUS       DAOPHV010000682         1477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_125956_length_1477_cov_3.942335, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000682 DAOPHV010000000
VERSION     DAOPHV010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125956_length_1477_cov_3.942335"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..1054)
                     /locus_tag="PLQ29_13330"
     CDS             complement(218..1054)
                     /locus_tag="PLQ29_13330"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HPG87675.1"
                     /translation="MKRCTRILSLIAVAALAFACAEPDGVSVGFALTDAPIDESLVTA
                     VNVTVSEVAVNESADASLADGDGSWKKVTIDPPVTINLLDLQGGLYEDLGDPIVLAGG
                     TQINQIRLGVDAVQVMEGDVAAVTTMPSATGLKIVNAFRVPPSGAITVTVDFDVRKSV
                     VLTGSTYVVKPVLRAVVDNEAGQLTGTVPAGVVTVYAYTDGSYVDAEATTANADDLYF
                     TGAYTSATVKADATYVLAFMDAGAYDLYGVDSTGAVIAELQDAVVTEGDVTDHAHLTA
                     IP"
     gene            1161..>1477
                     /locus_tag="PLQ29_13335"
     CDS             1161..>1477
                     /locus_tag="PLQ29_13335"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HPG87676.1"
                     /translation="MRVDDETLAARAKDGDERTFEELALRYSDRLYAFCRARLGSEND
                     AKDSAQDALVRAYRSLPSYDSSKPWSAWVFSIAANRVKSRYAAASSAERLALRVSAEQ
                     AVA"
BASE COUNT          223 a          488 c          499 g          267 t
ORIGIN      
        1 tcgataacct acccatgcac ggcacgatgg gccgccctga aaaccttgct cgtactcagg
       61 cctcgcattt ccagggagga gggtgaccaa gagacggcgg ccctcgcgtg agcgagggcc
      121 ccgcctcttg ggcaggaccc tccgagctac tatacgaggc ctgtatacta tcatgagccg
      181 cagggcggcg cgtcgtgtga tttccgcggc gcgtcgttta gggaatcgcc gtcaggtgcg
      241 cgtgatcggt cacgtcccct tcggtgacga cggcgtcctg cagctccgcg atgacggcgc
      301 cggtggagtc gacgccgtac aggtcgtagg cgccggcgtc catgaaggcg agcacgtagg
      361 tggcgtccgc cttgaccgtc gcgctcgtgt aggcgccggt aaagtacagg tcgtcggcgt
      421 tagccgtcgt ggcctcggcg tccacgtagc tgccgtcggt atacgcgtag accgtgacga
      481 ccccggccgg tacggtgccg gtaagctggc ctgcctcgtt gtccactacc gctcgcagga
      541 ccggcttgac gacgtaggtc gagccggtca gcacgaccga cttgcgcacg tcgaagtcga
      601 cggtcaccgt gatggccccg gatggcggca cgcggaaggc gttgactatc ttgaggccgg
      661 tggcggacgg catggtcgtg accgctgcga cgtcgccttc catgacctgg accgcgtcga
      721 cgccgagcct gatctggttg atctgcgtgc cgccggccag gactatcggg tcgccgaggt
      781 cctcgtatag cccgccctgc aggtcgagca ggtttatcgt gacgggcggg tctatcgtca
      841 ccttcttcca cgagccgtcg ccgtcggcca agctggcgtc ggcgctctcg ttgacggcca
      901 cctcgctgac cgttacgttg accgcggtga cgagcgactc gtcgatcggc gcgtcggtca
      961 gcgcgaagcc gaccgatacg ccgtccggtt cggcgcaggc gaacgcgagc gccgcgacgg
     1021 cgatcaggct gagtatccga gtacagcgtt tcatacgacc tccttgtacg ggacggccct
     1081 ggggccgcgt ccgtatagcc tgctataccc gacgcggccg tagtcggttg cgcatgaatc
     1141 cgtgctattt tctccttgcc atgcgggtgg acgacgagac cctggccgcg cgcgccaagg
     1201 atggcgacga gcggaccttc gaggagctgg cgctccgcta ttccgatcgc ctctacgcgt
     1261 tctgccgggc caggctcggc tcggagaacg acgccaagga ctcggctcag gacgcgctcg
     1321 tgcgcgcgta ccgttcgctg ccctcgtacg atagctcgaa gccatggtcc gcctgggtct
     1381 tctcgatagc cgccaaccgg gtcaagtcgc gctacgccgc cgcctcgtcg gcagaacgcc
     1441 tcgcgctcag ggtatccgcc gagcaggccg tggcgga
//
LOCUS       DAOPHV010000683         1475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_126199_length_1475_cov_4.138732, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000683 DAOPHV010000000
VERSION     DAOPHV010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126199_length_1475_cov_4.138732"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /gene="rplC"
                     /locus_tag="PLQ29_13340"
     CDS             <1..543
                     /gene="rplC"
                     /locus_tag="PLQ29_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPG87677.1"
                     /translation="VGPNKVVARKTAEKDGYDAVVLGYKDMKKVQVTKPYAGQFPEGV
                     APTKVLRELRDFSGEAEVGKELGVSLFEGTRFVDVIAVSKGKGFQGVVKRWGFGGGRN
                     THGSKFHREPGSTGMSTYPHRTFKNKKMPGRMGRERVTVQNLKLVKVDVENGFMLIRG
                     AVPGPRNCVVVVRSAVKKAK"
     gene            556..1191
                     /gene="rplD"
                     /locus_tag="PLQ29_13345"
     CDS             556..1191
                     /gene="rplD"
                     /locus_tag="PLQ29_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPG87678.1"
                     /translation="MEGKVFSVQGKELRSIELSDDVFGLPVNEDVIWYAINNELANAR
                     VGTADTKTRSDVHGTNRRPYAQKGTGRARQGDVKSPIYVGGGIVFGPHPRDYSYSINK
                     KAKRLAMKSILSLKAQGSTMKIVEDFSVDSGKTKDLASILAGLVPEGKAERTVVILKD
                     DDSMVKRAGRNIPWLHFLSYNRLRAHDLFYGRTVIVMESAVKSLNEFYADK"
     gene            1206..>1475
                     /locus_tag="PLQ29_13350"
     CDS             1206..>1475
                     /locus_tag="PLQ29_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPG87679.1"
                     /translation="MQYESILIEPVLSEKTNLLREEGQYVFRVDPRATKTQVKEAVKR
                     LFEVHPISCTIMNVKGKPKRLRYRSGYTSSWKKAVVRLAKGETIKA"
BASE COUNT          327 a          442 c          466 g          240 t
ORIGIN      
        1 gtagggccga acaaggttgt cgcccgcaag accgcggaga aggacggcta tgacgccgtc
       61 gtgctcggct acaaggacat gaagaaggtc caggtgacca agccgtacgc tggccagttc
      121 cccgagggcg tggcgccgac gaaggttctt cgcgagcttc gcgacttctc gggcgaggcc
      181 gaggtcggta aggagctcgg cgtatctctg ttcgagggta cgcgtttcgt cgacgtgatc
      241 gccgtgagca agggaaaggg tttccagggc gtcgtgaaac gctgggggtt cggaggcgga
      301 cgtaatacgc acggctccaa gttccaccgc gagcccggct cgaccggcat gagcacctac
      361 ccgcacagga cgttcaagaa caagaagatg cccggccgca tgggccgaga gcgcgtcacc
      421 gttcagaacc tgaagctggt caaggtcgac gtcgagaacg gcttcatgct gatccgcggc
      481 gccgtccccg gtccgcgcaa ctgcgtcgtc gtcgtgcgtt cggccgtcaa gaaagctaag
      541 tgaggataga ctgatatgga aggcaaagtc ttttccgtac agggcaagga gcttcggtcg
      601 atcgagctgt ccgatgacgt gtttggcctg ccggtcaacg aggacgttat ctggtacgcc
      661 atcaacaacg agctcgcgaa cgcccgcgtc ggcaccgccg acacgaagac ccgatccgac
      721 gtccacggca ccaaccgcag gccctatgcc cagaagggca ccggccgcgc ccgccagggc
      781 gacgtcaagt cgccgatcta cgtaggcggc ggtatcgtct tcggccccca cccgagggac
      841 tactcgtact cgatcaacaa gaaggccaag cgcctcgcga tgaagagcat cctgagcctc
      901 aaggcgcagg ggtcgacgat gaagatcgtc gaggacttct cggtcgacag cggcaagacc
      961 aaggacctcg cgtccattct ggccggtctc gtccccgaag gcaaggctga gaggaccgtc
     1021 gtgatcctga aggacgacga ctcgatggtt aagcgcgcgg gtcgcaatat cccgtggcta
     1081 cacttcctgt cgtataaccg cctccgcgct cacgacctgt tctacggacg gaccgtgatc
     1141 gtgatggagt ccgcggtgaa gagcctcaac gaattctacg cggacaaata aggaaggctg
     1201 aagcaatgca gtacgaaagc atactgatag agcctgtcct cagcgagaag acgaaccttc
     1261 tgcgcgaaga gggacagtac gttttccgcg ttgacccgag ggcgaccaag acccaggtca
     1321 aggaagccgt aaagcggctc ttcgaggtgc acccgatctc gtgcaccatc atgaacgtca
     1381 agggcaagcc gaagaggctt cgctaccgct ccggatacac ctcgtcgtgg aagaaagccg
     1441 tcgtccgcct cgccaagggc gagaccatca aggcg
//
LOCUS       DAOPHV010000684         1473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_126499_length_1473_cov_2.697461, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000684 DAOPHV010000000
VERSION     DAOPHV010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126499_length_1473_cov_2.697461"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLQ29_13355"
     CDS             <1..360
                     /locus_tag="PLQ29_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87680.1"
                     /translation="MSSEARHFAKLFRVELDDLIDEIGLLVDRMENRYERGELTEYVH
                     LENAAFYKRMSDSLLKFAGIVDGIDPSIYKCMADIEADLLARSRDFTIKLEDPEAVHI
                     LLRRKLDKVKKFISSDE"
     gene            400..1020
                     /locus_tag="PLQ29_13360"
     CDS             400..1020
                     /locus_tag="PLQ29_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HPG87681.1"
                     /translation="MDIRVQELLEKIKRDGVEHAEAEAAKILADAEKKRADVIAAAEK
                     EAKAIVAKAESDAARAAESGRAALTQASRDLILAFSGEVQNLLAELVKAEVEASFDAG
                     TVKKALPTILEAWSKDGRDELSVLLPAKDLKDLEAFFKEKLAASFKKGVEFKPLKDAR
                     AGFRIVEKGGAAYYDFSAQAVAEMLGSYLNARLAAIAASSVAEAAK"
     gene            1049..>1473
                     /locus_tag="PLQ29_13365"
     CDS             1049..>1473
                     /locus_tag="PLQ29_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87682.1"
                     /translation="MATLPSLLAGASAPPLSHAEFLERSSRFLSEKDMAVLEGARLFI
                     PEDGSFPTAAGSSPLLARYYRWELSLRNELARLRAGRLQKPVEKHVKPGEPEWDGLKV
                     AQAAMQADDPLQGELLIERERWSFVESLAVGRFFDLEYLV"
BASE COUNT          302 a          414 c          487 g          270 t
ORIGIN      
        1 atgagtagcg aagcgaggca ttttgccaag ctgttcaggg tcgagctcga cgaccttata
       61 gacgagatag gcctgctcgt cgataggatg gagaatcgct acgagcgcgg cgagctgacc
      121 gagtacgttc acctggagaa cgccgcattc tataagagga tgtccgattc gctacttaaa
      181 ttcgcgggca ttgttgacgg aatcgatccg tcgatctata aatgtatggc tgatattgaa
      241 gcggacttgt tggctaggtc gagggatttt actataaaac tagaggatcc cgaggccgta
      301 catattttac tgaggcgaaa gctcgataaa gtcaagaaat tcatctcgtc ggacgaatga
      361 gcgccagcgt tctaactatc gaccgggaaa gaggatgcca tggatattcg ggtgcaggaa
      421 ctactggaga agatcaagcg cgacggcgtg gaacatgccg aggcagaggc ggcgaagata
      481 ctcgccgatg ccgagaagaa gcgggccgac gtcatcgcgg ccgccgagaa ggaagccaag
      541 gccatagtcg ccaaggccga gtccgacgcg gccagggccg ctgagtccgg cagggcggcg
      601 ctgacgcagg cgtcgcggga tctgatcctc gccttctcgg gcgaggtcca gaacctgctc
      661 gccgagctgg tcaaggccga ggtcgaagcc tcgttcgatg ccgggaccgt caagaaggcc
      721 ctgccgacca tcctcgaagc ctggtccaag gatggtcgag acgagctgtc ggtgctgttg
      781 cccgccaagg acctcaaaga cctcgaggcg ttcttcaagg agaagctcgc cgcctcgttc
      841 aagaagggcg tcgagttcaa gccgctcaag gacgcgagag ccgggttcag gatcgtggag
      901 aagggcgggg cggcctacta cgacttctcg gcccaggccg tggccgagat gctcggttcc
      961 tacctgaacg ccagactggc cgcgatcgcg gcctcgtccg tcgcggaggc ggcgaagtag
     1021 ggccgccatg tcttcctatt attacttcgt ggcgacgctc ccgtcgctgc tggccggcgc
     1081 ttcggccccg ccgctatctc acgccgagtt cctggaacgc tcctcgcgct tcctatccga
     1141 gaaggacatg gccgtgctcg agggggccag gctcttcatt ccagaggacg gctcctttcc
     1201 gacggcggcc ggctcgtcgc cgctactggc aaggtactat cgctgggagc tgtcattgcg
     1261 caacgagctg gcgagacttc gcgccgggag gctacagaaa ccggtcgaga aacacgtgaa
     1321 gccgggcgag ccggagtggg acgggctcaa ggtagcccag gccgcgatgc aggcggacga
     1381 tccgctccag ggcgagctat tgatcgagcg ggagcgatgg tccttcgtcg agtcgctcgc
     1441 cgtgggtcgg ttcttcgatc tagagtacct cgt
//
LOCUS       DAOPHV010000685         1471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_126796_length_1471_cov_2.443503, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000685 DAOPHV010000000
VERSION     DAOPHV010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126796_length_1471_cov_2.443503"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PLQ29_13370"
     CDS             <1..686
                     /locus_tag="PLQ29_13370"
                     /inference="COORDINATES: protein motif:HMM:NF015889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HPG87683.1"
                     /translation="GHRAGAPGARPDGGSGPVTDGSPRLGWEHFKGPSVLLVVVIVGF
                     VTGLALPELALDYGSVTGWVLNALLFMIGLQFAQSGISLRSAFVRADTALVPIATMVG
                     SLAGGLVVAAAFSISAGKALSLAAGFGWYSLSGVLLSDLGDPVLGSAAFLANMARESM
                     ALLLIPLLSRSKRPYTAVGAGGATAMDVTLPLIEQCLGPDSVPVSFTSGAILSLLVPV
                     LVPLFYGLG"
     gene            complement(719..>1471)
                     /locus_tag="PLQ29_13375"
     CDS             complement(719..>1471)
                     /locus_tag="PLQ29_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87684.1"
                     /translation="EDCKTEFSVVFTGKKSGMVNGLYKPLTREILIHNKNFENDGQLI
                     YTAIHEYAHHIHCERGGFVPGARSHTNEFWAIFHELLEKAEGLGLYENSFDSDPDFVE
                     MTGRIRALLPENGRVMLEFGKLVVEAEALCRKHLVRFEDYLDRAIGIPRTSAAAAMKA
                     YSFQVPAEIGWDAMKVVSGIRQSEVRGQAIEAFKSGKSPDTVKAMVRRDKPADDPSER
                     LQKERKRLERTIKNLQDRLTMVESELSKMAAD"
BASE COUNT          190 a          504 c          481 g          296 t
ORIGIN      
        1 gcggccatcg cgccggcgcg cccggcgcgc ggcccgacgg cggctcaggg ccggtgaccg
       61 acgggtcgcc gcggctgggc tgggaacatt tcaaggggcc gtcggtcctg ctcgtcgtcg
      121 tcatcgtcgg tttcgtgacc gggctcgccc taccggaact ggcgctcgac tacggctcgg
      181 tgaccggctg ggtgctcaac gcgctcctgt tcatgatagg gctccagttc gcccagtccg
      241 gcatatcgct gcgctccgcc ttcgtgcggg ccgacaccgc gctcgtgccg atagctacga
      301 tggtcggcag cctcgccggt ggactcgtcg tcgcggcggc cttctcgatc agcgccggca
      361 aggccctctc actcgcggcc ggcttcggct ggtatagcct ctcgggcgtg ctgctatccg
      421 acctcggcga cccggtcctc ggctcggcgg ccttcctcgc caacatggcc cgcgagtcga
      481 tggccctcct cctgataccg ctcctgtcgc ggtccaagcg gccgtacacg gcggtcgggg
      541 ccgggggggc caccgcgatg gacgtgacgc tcccgctcat cgagcagtgc ctcggcccgg
      601 actcggtgcc ggtcagtttc acgagtgggg cgatactatc gctcctcgtg ccggtgctgg
      661 tgccgttgtt ctacggcctc gggtagcgtc ccctggctgc ggcttagtcg gccgaggcct
      721 agtcagccgc catcttcgac agctcggact cgaccatggt caggcggtcc tgcaggttct
      781 tgatggtccg ctcgagccgc ttgcgctcct tctgcaggcg ctccgacggg tcatcggcgg
      841 gcttgtcccg cctgaccatg gccttgacgg tgtcggggct cttgcccgac ttgaacgcct
      901 cgatagcctg gccgcgaacc tcggactgcc tgatgccgga gacgaccttc atcgcgtccc
      961 agcctatctc ggcgggtacc tggaagctat aggccttcat cgcggcggcg gcgctcgtcc
     1021 tcgggatacc gatggcgcgg tccaggtaat cctcgaagcg gaccaggtgc ttacggcaca
     1081 gcgcctccgc ttcgacgacg agcttgccga actcgagcat cacgcggcca ttctccggca
     1141 gaagggccct gatgcgcccg gtcatctcga cgaagtccgg gtcggagtcg aagctgttct
     1201 cgtacagccc caggccctcg gccttttcga gcagctcgtg gaagatggcc cagaactcgt
     1261 tcgtgtgcga cctggcgccg ggtacgaagc cgcctcgctc gcagtggata tggtgggcgt
     1321 actcgtggat ggccgtatag atcagctggc cgtcgttctc gaagttcttg ttatggataa
     1381 gtatctccct ggtcaggggc ttgtagaggc cgttgaccat accgctcttc ttgcccgtga
     1441 acacgacgct gaactccgtc ttgcagtcct c
//
LOCUS       DAOPHV010000686         1469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_126998_length_1469_cov_4.186704, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000686 DAOPHV010000000
VERSION     DAOPHV010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126998_length_1469_cov_4.186704"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PLQ29_13380"
     CDS             <1..614
                     /locus_tag="PLQ29_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87685.1"
                     /translation="SAPQASAAASDGRQEAPAQRRKPARFSSVTADVGSAATIAVFLG
                     NNYDIDLYTHAEEDSEAVSGFSYGISGIFPSGLFYLSAAASLTDTEHEDYSWYSYTQS
                     VEASAGAGLDFMVGPVMLYAGARLGYLMLTWTEEYYDYSTNEGTWSGLAYGFETGADA
                     RLGRLVLGLRYSFVMGTLSEIYEDWVDLDAATGSLSLRAGFAF"
     gene            complement(673..>1469)
                     /locus_tag="PLQ29_13385"
     CDS             complement(673..>1469)
                     /locus_tag="PLQ29_13385"
                     /inference="COORDINATES: protein motif:HMM:NF021943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87686.1"
                     /translation="STLAHEFQHMIHFYQKGVLRGGDYFNEATWIDELCAMQVEDLLS
                     DKLGVAGPRGVYPLDYSAGASGNVYGRLREYLPWPDLSLEAWPATDDELISSYYSLAY
                     AFGAYLTRNYGGSEFVRRLVQSASTDAGAVAAAASAFSGRDESIVGLLRRWGAAVLLS
                     HRTDAPAYYRYNSGGAFSSSSGGLTYNLGSINMYNYLCLDAIDTDGDDIGDADLAEPY
                     AFSSATIGDLYGGAYSNAFMDLGDPSSSSDWRFVLPAGMYATVVID"
BASE COUNT          250 a          464 c          504 g          251 t
ORIGIN      
        1 cgtccgcccc gcaggcctcg gccgccgcgt ccgacggccg gcaggaggct cctgcgcagc
       61 ggaggaagcc ggcccgcttc tcgagcgtca ccgccgacgt cggctccgcg gcgacgatag
      121 cggtcttcct aggaaacaac tatgatatcg atctctatac acacgccgaa gaggattcag
      181 aagcggtctc tggtttctcg tacggcatat ccggaatctt cccgtccggc ctgttctacc
      241 tgtcggccgc cgcgtccctg accgatacag agcatgagga ctacagctgg tacagctaca
      301 cgcagtccgt cgaggcaagc gccggagccg gcctcgactt catggtcggg ccggtgatgc
      361 tctacgccgg agccaggctt ggctacctga tgctcacctg gaccgaggaa tattacgact
      421 attcgacgaa tgagggaacc tggtcggggc tggcctacgg cttcgagacc ggtgccgacg
      481 cgcggctcgg gcgactggtg ctgggccttc gttactcatt cgtgatgggc accttatccg
      541 agatctacga ggactgggtc gacctcgacg cggcgaccgg ctccctgtcc ctgcgggcgg
      601 gcttcgcgtt ctagccccgc ctgcgatttg atcgacgcgc ccgaccggta tcgttccggc
      661 cgggcgtttt agttagtcga tgacgacggt ggcgtacatg ccggccggca gcacgaagcg
      721 ccagtcggac gacgacgagg ggtcgccgag gtccatgaag gcgttcgagt aggcgccgcc
      781 gtacaggtcg cctatcgtcg cggagctgaa cgcgtagggc tcggccaggt cggcgtcgcc
      841 tatatcgtcg ccgtcggtat cgatggcgtc caggcatagg tagttgtaca tattgatcga
      901 gccgagatta taggtcaggc cgcccgagct cgacgagaac gcgccgcccg agttgtagcg
      961 gtagtaggcc ggcgcgtcgg tccgatggga caggaggact gccgcgcccc agcgtctgag
     1021 cagcccgact atcgactcgt cgcgcccgga gaacgccgac gcggcggccg cgacggcccc
     1081 ggcatcggtc gaggccgact gcacgagacg gcgcacgaac tccgagccgc cgtagttgcg
     1141 ggtcaggtag gcgccgaagg cgtaggcgag cgagtagtac gaggagataa gttcatcatc
     1201 ggtcgcgggc caggcttcga gcgacaggtc cggccagggc aggtactcac gcagccgccc
     1261 gtagacgttg cccgacgcgc cggcgctgta atcgagcggg tagacgccgc gcggcccggc
     1321 gacgccgagc ttgtctgaga gcaggtcctc cacctgcatc gcgcacagct cgtcgatcca
     1381 ggtggcctcg ttgaagtagt cgccgccgcg cagtacgccc ttctggtaga agtggatcat
     1441 gtgctggaac tcgtgggcga gggtggaga
//
LOCUS       DAOPHV010000687         1469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_127019_length_1469_cov_3.304102, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000687 DAOPHV010000000
VERSION     DAOPHV010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127019_length_1469_cov_3.304102"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLQ29_13390"
     CDS             complement(<1..432)
                     /locus_tag="PLQ29_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HPG87687.1"
                     /translation="MLGKGPGMDATGKLSILADAAKYDASCSSSGSARDNTPGGLGNG
                     AVGGICHSWAADGRCVSLLKVLLSNACVYDCAYCVNRRSNDVPRAGFEPEELVRLVID
                     FYKRNYIEGVFLSSGVLGCPDHTMERMIRVAKTLRVRGRWNG"
     gene            complement(491..>1469)
                     /locus_tag="PLQ29_13395"
     CDS             complement(491..>1469)
                     /locus_tag="PLQ29_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87688.1"
                     /translation="DDETRRFQTALFYRTNERSIRIMSDHPAIRERVFGPLRRLPDAA
                     MFEHFLGLGSGKPTFTKIRSDAAPLPIGLDGSCDKVEVTSVIGSRAFGLYCRRRFPLG
                     LFVSPPEWYFSMKYFVNRVYDYVCAMRVHGHSLAAIGFDAPRAVDGVDPDYRFLRNAH
                     KLMLHLQELIRTRVGAYGVEVDYSWMSATRFKRLVDIDGDGFRRDFDAMVLDGGLLMP
                     SEAERYRVLRGRVLAKSGDRVMEGRAQDLASLPEGLRYETIYQDADRFKIRIPYSWSD
                     LAFFAFSAIGSRMARIVEGRLMKALPALGMPPADARRYQLVMHEQARRR"
BASE COUNT          273 a          451 c          481 g          264 t
ORIGIN      
        1 gccgttccag cgtccccgga cgcgcagggt cttggcgacg cggatcatgc gctccatcgt
       61 atgatcggga cagccgagga cgccggacga gaggaagacg ccttcgatgt agttgcgttt
      121 atagaagtcg atgacgaggc gtacgagctc ctcgggctcg aacccggccc ggggaacgtc
      181 gttggatcgg cgattgacgc agtaggcgca gtcgtagacg caggcgttcg acaggagcac
      241 cttgagcagg gagacgcagc ggccgtccgc ggcccagcta tggcaaatac ccccgaccgc
      301 cccgttaccc agccctccgg gtgtgttgtc cctggcgcta ccggacgatg aacacgaggc
      361 gtcgtatttg gctgcgtccg ctagtatcga tagcttcccc gttgcatcca tgcccggacc
      421 tttcccgagc atgatactat acgaatgtat agtagtcaac gagatcggct atggaacggc
      481 cagctcagca tcagcgcctg cgcgcctgtt catgcatgac gagctggtag cgtctggcgt
      541 cggcgggagg catgccgagc gccggaaggg ctttcatgag tcgtccttcg acgatgcggg
      601 ccatcctgga gcctatcgcg ctgaacgcga aaaacgcgag gtccgaccag gagtacggta
      661 tcctgatttt gaagcggtcc gcgtcctggt agatagtctc gtagcgtagg ccttcgggca
      721 ggctcgcaag gtcctgcgcc ctgccttcca tgactctgtc gccagatttg gcaaggacgc
      781 gcccgcgtag gacgcgatag cgttcggcct cgctcggcat gagcagcccc ccgtccagga
      841 ccatagcatc gaagtcgcgt cggaacccgt ctccgtctat gtcgacgagc cgcttaaacc
      901 tggtggcgga catccacgag tagtcgacct cgacgccgta ggcgcctacg cgcgtgcgga
      961 tgagctcctg taggtggagc atcagcttat gggcgttgcg taggaagcgg tagtccgggt
     1021 caacgccgtc aaccgcgcgc ggcgcgtcga agccgatcgc ggcgagcgaa tggccgtgga
     1081 cccgcatggc gcagacgtag tcgtagacgc ggttgacgaa gtacttcatc gagaaatacc
     1141 actccggcgg cgatacgaac agcccgagcg ggaagcgcct acggcagtag aggccgaagg
     1201 cccggctacc gatgaccgac gttacctcca ccttgtcgca gctaccgtcg agcccgatgg
     1261 gcagtggagc cgcgtccgag cgtatcttgg tgaaggtcgg cttgccggag ccgagcccca
     1321 ggaaatgctc gaacatcgct gcgtcgggca ggcgccgcaa ggggccgaac acccgctctc
     1381 gtatggccgg atggtcgctc atgatcctga tgctgcgctc attcgtccgg tagaacaggg
     1441 cggtctggaa gcgcctcgtc tcgtcgtcc
//
LOCUS       DAOPHV010000688         1462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_127896_length_1462_cov_4.070362, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000688 DAOPHV010000000
VERSION     DAOPHV010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127896_length_1462_cov_4.070362"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="PLQ29_13400"
     CDS             <1..1227
                     /locus_tag="PLQ29_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG87689.1"
                     /translation="PAAPPAWTNLFAAEKSATTAYLNDPIVEDVDDPDFGLARVYTFK
                     YNYKYDKSPLDIIFRAKGDGMLFMNLSVIRPDGETVYLGQVYEDGLAGDDIRFTVDTD
                     ARSSVYEFIRAHASEEAIANSDSSNVKATDIIFAKAGADMLSAREPLKGEYLYKVTIP
                     ASLFEDEANRIDEAKLIVTGRVSGVLGTDTSKRDIYSGIIAGLKWALFIGLVTSVISV
                     LVGVLYGIISAYFGGTTDTIMSFIFEVFVSMPMLPILIVLSATFKPSIWIIIGAMIVF
                     YWVGPVKTVRSMALQIKEETYIEAAKALGAGKWRIIFKHMAPILLPYSFANMALSVPG
                     AIVAESSLSLLGLGDPSIISWGQILHDAQLSGATLNGLWWWIVPPGLFIAVMGMTFAF
                     LGFAMDKILHPKLRTR"
     gene            1231..>1462
                     /locus_tag="PLQ29_13405"
     CDS             1231..>1462
                     /locus_tag="PLQ29_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG87690.1"
                     /translation="MDKLLEVKNLKLYYHTSKGAVRALDDVSFDLYKGETLGLVGESG
                     CGKTTTGTALLRMPTAPGRYDGGQILIDGRDII"
BASE COUNT          281 a          495 c          421 g          265 t
ORIGIN      
        1 cccgcggccc cgcccgcctg gaccaaccta ttcgccgccg agaagtcggc cacgaccgcg
       61 taccttaacg atccgatcgt cgaggacgtg gacgatccag acttcggcct agccagggtc
      121 tataccttca agtacaacta taagtacgac aagtcgccgc tcgacatcat ctttagggcc
      181 aagggcgacg gcatgctgtt catgaacctt tcggtgatcc ggcccgacgg cgagacggtc
      241 tacctcggcc aggtctacga ggacgggctc gcgggcgacg acatacgctt caccgtcgac
      301 acggacgccc gttcgtcggt ctacgagttc atccgggccc acgcgagcga ggaggccata
      361 gccaactcgg actcctcgaa cgtcaaggct accgacatca tcttcgccaa ggccggcgcg
      421 gacatgctgt ccgccaggga gccgctcaag ggcgagtacc tgtacaaggt cactatcccc
      481 gcgtccctgt tcgaggacga ggccaaccgc atcgacgagg ccaaactgat agtcaccggc
      541 cgcgtctccg gcgtgctcgg caccgacacc tcgaagcgcg acatctactc gggcatcata
      601 gccggcctca agtgggcatt gttcatcggc ctcgtcacga gcgtcatctc ggtgctcgtc
      661 ggcgtactct acggcatcat cagcgcctac ttcggcggca cgaccgacac gatcatgtcc
      721 ttcatcttcg aggtattcgt gtcgatgccg atgttaccga tcctgatcgt gctgtcggcg
      781 accttcaagc cgagcatctg gatcatcatc ggcgcgatga tcgtgttcta ctgggtcggc
      841 ccggtcaaga ccgtcaggtc gatggccctg cagatcaagg aagagaccta tatcgaggcg
      901 gccaaggccc tcggcgccgg caagtggcgc attatcttca agcacatggc cccgatactc
      961 ctgccctact cgttcgccaa catggcgctg tcggtgccag gcgcgatcgt cgccgaatcg
     1021 tcgctgtccc tcctgggcct gggcgacccg agcatcatct cctggggtca gatactccac
     1081 gacgcgcagc tctccggcgc cacgctgaac gggctctggt ggtggatcgt cccgccggga
     1141 ctcttcattg ccgtgatggg catgacgttc gccttcctgg gtttcgccat ggacaagatc
     1201 ctgcacccga aactccgcac gaggtaaacc atggacaaac tactggaagt caagaacctt
     1261 aagctgtact accacacctc gaaaggggcg gtacgcgccc tcgacgacgt cagcttcgac
     1321 ctgtacaagg gcgagaccct cgggctcgtc ggcgagtcgg gctgcggcaa gaccaccacc
     1381 ggcaccgcgc tgctgcgcat gcccacggct cccgggcgct acgacggcgg ccagatcctg
     1441 atcgacggca gggacatcat cc
//
LOCUS       DAOPHV010000689         1456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_128752_length_1456_cov_2.685225, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000689 DAOPHV010000000
VERSION     DAOPHV010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128752_length_1456_cov_2.685225"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PLQ29_13410"
     CDS             <1..643
                     /locus_tag="PLQ29_13410"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPG87691.1"
                     /translation="SFLQALAPYVSIAVENALIHDRLEVLNRALSDEKRRLERATLKI
                     SHLANHDTLTGLPNRRLLVELMDKSVETARRTGSKIGVVFMDLDDFKPINDQFGHTAG
                     DSALVAMADRIRGLVRASDIVARIGGDEFLAIITNIQHRDDVERVARKVIEECGTPLT
                     FSGASWKVGVSIGIALFPDDGESIEDLVNRADSAMYRVKHMSKNAYSFWGDSA"
     gene            790..>1456
                     /locus_tag="PLQ29_13415"
     CDS             790..>1456
                     /locus_tag="PLQ29_13415"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPG87692.1"
                     /translation="MASNNDKHVRPESSDRRPRGNSFIYVGTVVLLVITVVAFVFVPA
                     MGSMGGEGGDLTFGSWNGKPIAFAQGGYFASQVQQVKAQYESQGYKDSGDQFFAYQVW
                     RRAFENTAVHLALLDYADKVGIAASESYIDAQMVKHPAFQESGAFSKRKYREAGDSYK
                     LSLRKDIALSSVKSRYVSDSVGYVTSQAEIAFLKAMAQSRRSIEFVAIPFASSPDEER
                     AAFA"
BASE COUNT          286 a          479 c          435 g          256 t
ORIGIN      
        1 gtccttcctc caggccctgg ctccgtatgt cagcatcgcc gtcgagaacg ccctgatcca
       61 cgaccgcctg gaggtcctga accgcgccct ctcggacgag aagcggcggc ttgagcgcgc
      121 tacgctcaag atcagccacc tggctaacca cgacacgctg acggggctcc cgaaccggcg
      181 cctcctcgta gaactgatgg ataagagcgt tgagacggcc aggcgcaccg gaagcaagat
      241 cggcgtcgtg ttcatggacc tggacgactt caagccgatc aacgaccagt tcggccatac
      301 ggccggagac agcgccctcg tcgcgatggc cgaccgcatc cgcggcctcg tgcgggcctc
      361 cgacatcgtg gcgcgcatcg gcggcgatga gttcctggcc atcatcacga acatccagca
      421 ccgcgacgac gtggagcgag tcgcccgcaa ggtcatagag gagtgcggaa cccccctgac
      481 cttctccggc gcctcctgga aggtgggcgt atcgataggc atagcgctct tccccgatga
      541 tggtgagtcc atcgaggacc tcgtcaaccg cgccgactcg gccatgtacc gggtcaagca
      601 tatgagcaag aacgcctact cgttctgggg tgacagcgcc tagaaacgcc cggatcggac
      661 tcgattgaca ctgccatagc cccgtgctat gtttttccgc gctttacgtc gagggattgt
      721 tgctaacgag agtaccgtca ataccggccg agcgaccgtc cacccatcat atgttccagg
      781 aaggtttcga tggcatctaa caacgacaag cacgtcaggc ccgaatcgag cgaccgtcgc
      841 cctcgaggca attcattcat ctacgtaggc accgtcgtgc tcctcgtcat aacggtcgtc
      901 gcgttcgtgt tcgtccccgc gatgggcagc atgggtggcg agggcggcga cctgaccttc
      961 ggctcctgga acggcaagcc gatagcgttc gcgcagggcg gctacttcgc ctcccaggtc
     1021 cagcaggtca aggcccagta cgagagccag ggctacaagg actccggaga ccagttcttc
     1081 gcgtaccagg tatggcgcag ggccttcgag aacaccgccg tccacctcgc cctgctcgac
     1141 tacgccgaca aggtgggcat agccgccagc gagtcataca tagacgccca gatggtcaag
     1201 catccggctt tccaggaaag cggggccttc tcgaagcgca agtaccgcga ggcgggcgac
     1261 tcgtacaagc tatcgctccg caaggatata gccttgtcgt cggtcaagtc ccgctacgtc
     1321 tcagactcgg tgggctacgt gacgagccag gccgagatcg ccttcctgaa ggccatggcc
     1381 cagtcgaggc gctccataga attcgtggcc atcccgttcg cctcgtctcc ggatgaggag
     1441 cgagcggcct tcgccg
//
LOCUS       DAOPHV010000690         1453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_129121_length_1453_cov_4.708155, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000690 DAOPHV010000000
VERSION     DAOPHV010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129121_length_1453_cov_4.708155"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="PLQ29_13420"
     CDS             complement(<1..1256)
                     /locus_tag="PLQ29_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HPG87693.1"
                     /translation="MDKRGAREFVIELGALGGTAEEGGRPAGRVEFHVSKAARDRFGL
                     AGVPYATAGGVTFADYHEARAFAAAINDHAAANGGPVVKAGAINAMGLLDEILHAVCA
                     LYRERVDAAAFDTALASAGAAIGASRVRSSLVAFSRAFPTGDVYAGRLEPEAWLDGLD
                     DGRCKRAVALEELLLLRLANENPALEPYRFLFDDAALRSRAPVDASLDAAEAALAAMP
                     PFGPDSLSLPDLLRAPMRASPRSLAGQLEYVRRRWGLLLGDRLSRLLGAADMLAEDDR
                     QGFAPGPGPIRVPSYAALDAEYERFSEDKDWMPRVVMMAKSTLVWLDQLSKAYGRAIR
                     TLDAIPDEELDLLAARGFNALWLIGLWERSQASRRIKELCGNPEAAPSAYSLFDYEIA
                     WELGGWDALGSLRERAGKRASYPLPH"
BASE COUNT          202 a          490 c          551 g          210 t
ORIGIN      
        1 cagtgtggga gcggatatga agcgcgcttt ccggcgcgtt cccgaagcga gccgagcgcg
       61 tcccagccgc cgagttccca ggctatctcg tagtcgaaca gcgagtaggc gctcggcgcc
      121 gcctccgggt tgccgcacag ctccttgatg cggcgcgagg cctggctgcg ttcccagagg
      181 cctatgagcc acagcgcgtt gaagccccgg gcggcgagca ggtcgagctc ctcgtcgggg
      241 atggcgtcga gggtgcgtat ggcgcggccg taggccttgg acagctggtc cagccagacg
      301 agggtgctct tggccatcat cacgacgcgg ggcatccagt ccttgtcctc gctgaagcgc
      361 tcgtactcgg cgtcgagcgc ggcgtaggac ggcacccgta tcggccccgg ccccggcgcg
      421 aagccttgcc tgtcgtcctc ggcgagcatg tcggcggcgc cgagtagcct ggacaggcgg
      481 tcgccgagca ggaggcccca gcggcggcgc acgtactcga gctggccggc gagcgagcgg
      541 gggctggccc gcatcggcgc gcgtaggagg tcgggcaggc tgagcgagtc ggggccgaag
      601 ggcggcatgg cggcgagcgc cgcctcggcc gcgtcgaggc tggcgtccac cggcgcgcgc
      661 gagcggagcg cggcgtcgtc gaacaggaag cggtagggtt ccagggccgg gttctcgttg
      721 gccagcctga ggaggaggag ctcctcgagg gctacggctc gcttgcagcg gccatcgtcg
      781 agaccgtcga gccaggcttc cggctccagg cggcccgcgt agacgtcgcc ggtcgggaag
      841 gcgcgcgaga acgctacgag gctcgagcgg acccggctcg cgccgatagc tgcgcctgcc
      901 gaggcgagcg ccgtatcgaa agccgccgcg tcgacccgtt cgcggtacag cgcgcagacg
      961 gcgtgcagga tttcgtcgag gaggcccatc gcgttgatcg cgccggcctt gacgacgggt
     1021 ccgccgttcg ccgccgcgtg gtcgttgatg gccgcggcga aggccctggc ctcatggtag
     1081 tccgcgaagg tcaccccacc ggcggtcgcg tagggtacgc cggccaggcc gaagcggtcg
     1141 cgagccgcct tggatacgtg gaattccacc cggccggcgg gacggccgcc ctcctcggcc
     1201 gtcccgccga gggctccgag ctcgatcacg aattccctgg cgccgcgctt gtccatcgtt
     1261 cgtactccgt tcctgccgtc gcgacgctcg atcgcgccga cccgcccttc gatgataacc
     1321 gatgcgccgg gcggggacaa ggacgggttc ttccgcgcgg tccgtcccta tcgggatgcc
     1381 ctctgggcga cgagccaacc gatgccgtag aggacgggca ggtggacgag gtacacgggg
     1441 agcgagagcc tgc
//
LOCUS       DAOPHV010000691         1453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_129144_length_1453_cov_3.552933, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000691 DAOPHV010000000
VERSION     DAOPHV010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129144_length_1453_cov_3.552933"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLQ29_13425"
     CDS             complement(<1..350)
                     /locus_tag="PLQ29_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87694.1"
                     /translation="MFIALAAASALFYGLGDFSGGYAAGKSKVLSVLVVSQACGLATA
                     LLAVAVTQPAPPGFVDLVWGLLGGVAGAFGIVTLYRGIAKGVVAIVSPTAALVSAALP
                     IAVGLALGDEPGSLA"
     gene            452..1357
                     /locus_tag="PLQ29_13430"
     CDS             452..1357
                     /locus_tag="PLQ29_13430"
                     /inference="COORDINATES: protein motif:HMM:NF009888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HPG87695.1"
                     /translation="MIDYRLLEAFAAVLDLGGFERAADGLGLTQSAVSQRVKALEDEM
                     GRMLVVRDTPPRPTPAGERILRHYRQVSELEKDATRDLGIRDESGFRRLPVAVNADSL
                     SVWFLDAVAPFLLDERVALELLVDDQEQTLRFLRSGAAAGAISSEVQAVQGFRRVALG
                     AIRYRLVATPAFIRRWFPGGFDRDSAGRAPIIHFNRADGLQLRALERAFGEPRVKPPA
                     HYVPSTDKLAEAVRLGLGYAMMPEAQASRELGDGRLVELDERCRFETPLHWYYWNRPS
                     EPLERFTEALVSGATRLLGPTLRRW"
BASE COUNT          252 a          500 c          474 g          227 t
ORIGIN      
        1 gctagcgatc ccggctcgtc gccgagggcg aggccgacgg cgatcggcag cgcggcgctc
       61 acgagggcgg cggtcggcga gacgatggcg acgactccct tggcgatgcc gcgatatagg
      121 gtgacgatac cgaaggcgcc ggccacgcct cccagtaggc cccagaccag gtcgacgaat
      181 cctggcggcg ccggctgagt gacggcgacg gccagcaggg ccgtagccaa tccgcaggcc
      241 tgggacacga ccaataccga caagaccttg ctctttccag cggcgtatcc gccggagaag
      301 tcgccgaggc cgtagaacag ggccgacgcc gcggctaagg ctataaacat tcattggctc
      361 cttacgcgag tttcgtcgtc agcatactgg aaccatgaat attagtgaaa ttaattattt
      421 gaatgctgca taaatcctgc tactatgcca tatgatagat tatcgcctcc tcgaggcctt
      481 cgcggcggtg ctcgaccttg gcggcttcga gcgagcggcc gacggcctcg gcctcaccca
      541 atcggcggtg tctcagcggg taaaggcgct ggaggatgag atgggccgta tgctcgtcgt
      601 gcgcgacacc ccgccccgcc ctacccccgc cggcgagcgg atcctcaggc attaccgcca
      661 ggtgtccgaa ctcgagaagg acgccacaag ggacctcggg atacgcgacg aatcgggatt
      721 ccgccgcctg cccgtcgcgg tcaacgccga ctccctatcc gtatggttcc tggacgccgt
      781 cgcgcccttc ctgctagacg agcgggtcgc gctcgagctg ctcgtcgacg atcaggaaca
      841 gacgctacgc ttcctgcgct ccggcgccgc cgccggcgcg atatcgtccg aggtccaggc
      901 cgtgcagggc ttcaggcgcg tagcgctcgg cgcgatacga tacaggctgg tcgcgacgcc
      961 agcgttcatc cgccgctggt tccccggagg cttcgaccgg gactcggccg ggcgagcgcc
     1021 catcatccac ttcaaccgag ccgacggcct gcagctacgc gcgctcgagc gggccttcgg
     1081 cgagccgcgg gtcaagccgc cggcccacta cgtgccgtcc acggataagc tcgcggaggc
     1141 cgtacggctc ggcctcggct acgctatgat gccggaggcg caggcgtcga gggaactcgg
     1201 agacggacgc ctcgtcgagc tcgacgagcg ttgccgcttc gagacgccgc tacattggta
     1261 ctactggaac cggccttccg agccgctcga gcgttttacc gaggcgctag tcagcggcgc
     1321 gacgaggctc ctcggcccca cgctccgccg ctggtagccg ccagaggaac gcggcatagc
     1381 ccagcgccaa cgcggtgatc gccccggcga cgataagcga ccctgccgtc ccggccggtt
     1441 cgacgccgaa tgc
//
LOCUS       DAOPHV010000692         1448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_129822_length_1448_cov_2.954056, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000692 DAOPHV010000000
VERSION     DAOPHV010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129822_length_1448_cov_2.954056"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PLQ29_13435"
     CDS             <1..759
                     /locus_tag="PLQ29_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HPG87696.1"
                     /translation="GGKCITEDLGIKLESLELSDLGRAKSIVVEKENTTIVEGAGKNS
                     EIQGRVNQIRRQIEETTSDYDREKLQERLAKLAGGVAVINVGAATEVEMKEKKHRVED
                     ALSATRAAIEEGIVPGGGLALIQAAIALDKNGADKLSDDAKVGYKIVKRALEEPIRQI
                     AENAGVDGAVVADRAKGEKKGIGFDAAKMEWVDMVKAGIIDPAKVTRSALQNAASVAS
                     LMLTTECAITDLPEPKAAPAGGMGGMGGMGGMDY"
     gene            1152..>1448
                     /locus_tag="PLQ29_13440"
     CDS             1152..>1448
                     /locus_tag="PLQ29_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87697.1"
                     /translation="MPTDELSIAALQYLFDTLYGAATLFLFSGVPATRRNVLTMALTA
                     SLAYAFVFVAIFTHGLEGLGIVYTLGVHLPCVLTVAIGLKRPLRITLMAFFFSYF"
BASE COUNT          279 a          488 c          453 g          228 t
ORIGIN      
        1 ggtggcaagt gcatcaccga ggacctcggc atcaagctcg agagcctcga gctgtccgac
       61 ctcggccgtg cgaagagcat cgtcgtcgag aaggagaaca ccacgatcgt cgagggtgcc
      121 ggcaagaact ccgagatcca gggccgcgtg aaccagatcc gccgccagat cgaagagacc
      181 acttccgatt acgaccgcga gaagctccag gagcgcctcg ccaagctcgc cggcggcgtg
      241 gccgtcatca acgtcggcgc cgcgaccgag gtcgagatga aggagaagaa gcaccgcgtc
      301 gaggacgccc tctccgccac ccgggccgcc atcgaggagg gtatcgtccc cggcggcggt
      361 ctagcgctga tccaggccgc gatcgccctc gacaagaacg gcgccgacaa gctgtccgac
      421 gacgcgaagg tcggttacaa gatcgtcaag cgcgccctcg aggagccgat ccgccagatc
      481 gccgagaacg ccggcgtcga cggcgcagtc gtcgccgatc gcgccaaggg cgagaagaag
      541 ggcatcggct tcgacgccgc gaagatggaa tgggtggata tggtcaaggc cggcatcatc
      601 gacccggcca aggtcacccg ctccgcgctc cagaacgccg ccagtgtcgc cagcctcatg
      661 ctgacgaccg agtgcgccat caccgacctg cccgagccca aggcggcccc cgccggcggc
      721 atgggtggca tgggcggcat gggcggtatg gattactgat ccaagcgcct gacatagcct
      781 gaacggccgc acccgtaagg gtgcggcttt ttattggtac cgcagcccag gccgcccatg
      841 cgatgaaacg tccgcgcctg aaggcgcggg cgcgaagcgc tctctggggc ggcggtcgcc
      901 gcgcggccta ccggccgcgc actacaagac aattcctcgc gacccgcggc gcccgaagga
      961 cgacgccacg gcggcatgga ttactgatcc aagcgcctga tatagcctga acggccgcgt
     1021 ccgcaagggt gcggcctttt tgttggtacc gcagcccagg ccgcccatgc gatgaaacgt
     1081 ccgcacctga aggcgcggtt gatagcctgc gctcgatccg atacgatgca ctcggagaaa
     1141 cgtgaccatt gatgcctacc gacgagctct cgatagcggc cctacagtac cttttcgata
     1201 ccttgtacgg cgccgcgacg ctgttcctat tctccggcgt tccggctacg agacggaatg
     1261 tcctgacgat ggccctgacc gccagcctgg cgtacgcgtt cgtattcgtg gcgatcttta
     1321 cgcatggact cgagggcctc ggcatcgtct acacactggg cgtccatcta ccctgcgtgc
     1381 tgacagtcgc catagggctc aagcgcccct tgcgaatcac cctcatggca ttcttcttct
     1441 catacttc
//
LOCUS       DAOPHV010000693         1447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_129915_length_1447_cov_4.403736, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000693 DAOPHV010000000
VERSION     DAOPHV010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129915_length_1447_cov_4.403736"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PLQ29_13445"
     CDS             complement(<1..846)
                     /locus_tag="PLQ29_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87698.1"
                     /translation="MKAGKNGTNKNARSGSGAAVAAGVIIGLALAAAAIWAMIRFMPA
                     REAEKAIVEDAPAAASEPSAPAGYSAVRGPAPGSEAPYDGELLAYARWLDTAGEHLVI
                     LSALRPADGPASAAVSSAETAETAETAETADSGVQARSYLLGGSGWALAAERFDPMPA
                     GAMAGYYGDAPWTGDFDGDGLGEALAAYWIDESPDAGAKRLSLLAFTAGAIASVDGST
                     RYDPADAPRLTPSAVAGEGWAALPAAIRGEAARLWSAAQFDLAEPPAFPDFARHARFD
                     GAVLKG"
     gene            complement(843..>1447)
                     /locus_tag="PLQ29_13450"
     CDS             complement(843..>1447)
                     /locus_tag="PLQ29_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87699.1"
                     /translation="RIVAAYPLGAPLDLMPGIPFLADDGVTVERPDFAVLLAIGWSRA
                     YPASVKLDEILSERAIERVVPLFDGTRDTDELLWKLSKALGRKAGEVRDIDLYAPAYR
                     TAIAEDPSSSAFFSAQEKARLDRWAPPRGLRLVLAAAPSDELVPFVTSARAYEWIKAE
                     DAEADVSLVRLESQSHGLAAVEGLLYALIDLDTIEGGEGL"
BASE COUNT          219 a          493 c          524 g          211 t
ORIGIN      
        1 gcccttgagc accgcgccat cgaagcgggc gtgcctggcg aagtccggaa aggccggggg
       61 ctcggccagg tcgaactggg cggcggacca gaggcgcgcg gcctcgccgc ggatcgccgc
      121 tggcaaggcg gcccagccct cgccggccac cgcgctcggg gttagccggg gcgcgtcggc
      181 cggatcgtag cgcgtcgagc cgtcgacgct ggctatagcg cccgccgtga acgccaggag
      241 ggataggcgc tttgctccgg catcagggga ctcgtctatc cagtaggccg cgagcgcctc
      301 gccgaggccg tcgccgtcga agtcgccggt ccagggcgca tcgccgtagt acccggccat
      361 ggcgccggcc ggcatcgggt cgaatcgctc ggcggccaat gcccagccgg agccgccgag
      421 gaggtagcta cgcgcctgga cgccggaatc ggcggtttcg gcggtttcgg cggtttcggc
      481 ggtttcggcg gacgataccg cggctgaggc cggtccgtcc gcgggccgta acgccgacag
      541 gatgacgagg tgctcgccag cggtgtccaa ccagcgcgcg taggccagta gctcgccgtc
      601 gtagggcgcc tcgctaccgg gagccggccc tcggacggcc gagtagccgg cgggggcgga
      661 cggctcggat gccgcggcgg gagcgtcctc gacgatagcc ttctctgcct cgcgagccgg
      721 catgaagcgt atcatcgccc agatcgccgc ggccgcgagc gccaagccga tgatcacgcc
      781 agcggcgacc gcggcgcctg agccgctacg ggcgttcttg ttcgttccgt tctttccggc
      841 tttcatagtc cctctccacc ttctatcgtg tccaggtcga tgagggcgta tagcaggccc
      901 tcgaccgcgg ccaagccgtg gctctggctc tccaaacgaa cgagcgaaac atcggcctcc
      961 gcatcctccg ccttgatcca ctcgtaggcg cgggcgctgg tcacgaacgg cacgagctcg
     1021 tccgagggag cggccgccag cacgaggcgc aggccccgtg gcggcgccca gcggtcgaga
     1081 cgggcctttt cctgggcgga gaagaaagcc gacgacgacg gatcctcggc tatcgccgtc
     1141 ctgtacgctg gcgcgtagag gtcgatgtcg cgcacctcgc ccgccttacg cccgagagcc
     1201 ttggatagct tccaaaggag ttcgtcggtg tccctggtac catcgaagag cggcacgacg
     1261 cgctcgatcg cccgctcgga cagaatctcg tcgagcttga ccgaagcggg gtaggcgcgg
     1321 gaccagccga tggccagcaa taccgcgaag tccggccgct cgacggtgac gccgtcgtcg
     1381 gcgaggaagg gtatgccggg catcaggtcg agcggagcgc cgagtggata cgcggcgacg
     1441 atgcgca
//
LOCUS       DAOPHV010000694         1433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_131943_length_1433_cov_3.351959, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000694 DAOPHV010000000
VERSION     DAOPHV010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131943_length_1433_cov_3.351959"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="PLQ29_13455"
     CDS             <1..1396
                     /locus_tag="PLQ29_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPG87700.1"
                     /translation="VPIKKEALERYLDYYESGCPVSKATVGEAKRYIPGGVQHNLAFN
                     YPFPLVIDRAEGAYLHDADGHRYIDFLQAGGPTILGSNDPVVRDQVIDVLRRVGPSTG
                     LFHEFELKLAKEICSHMPAVEMFRMLGSGTEAVMAAIRVARLATGKRRVIKVGGAYHG
                     WSDQLVYSLKIPKTGRFEAHGIPGSAQRLTDEVAPNDLGSLERKLAANRLRGGTACVL
                     IEPVGPESGTRPVAFDYNQGVRALCDKYGALLVFDEVVTAFRIGLEGAQGYFGVRPDL
                     TTFGKIVAGGYPSAGGLGGRKDLILMMAAGLESGKKRAYVGGTMAANPLSAAAGYFTI
                     KEIEKRDACAVAGRAGDRLTAGLKKLIAERGLPFVAYNQGSICHLETVGAMFIKMDYL
                     KIVRVLKEIKIRKKMMETYGAAYAAEGIVTLAGSRMYTSGADTDAVIDDALARFGRVF
                     DNVDVEAMNRGAAE"
BASE COUNT          250 a          501 c          472 g          210 t
ORIGIN      
        1 ggtgccgatc aagaaggaag cgctcgagcg ctacctcgat tactacgaga gcggctgccc
       61 agtctccaag gccacggtag gcgaggccaa gcgctacata cccggcggcg tccagcacaa
      121 cctggccttc aattaccctt tcccgctcgt catcgaccgc gccgagggcg cctacctgca
      181 cgacgccgac gggcaccggt atatcgattt cctccaggcc ggcggcccga ctatcctcgg
      241 cagcaacgac ccggtcgtcc gcgaccaggt tatcgacgtg ctcaggcgcg tcggcccgtc
      301 gaccggcctg ttccacgagt tcgagctcaa gctggccaag gagatctgct cccacatgcc
      361 cgccgtcgag atgttccgca tgctcggctc gggtacggaa gccgtaatgg ccgccatccg
      421 cgtggctagg ctcgcaaccg gcaaaaggcg cgtcatcaag gtcggcggcg cctaccacgg
      481 ctggagcgac cagttggtct acagcctcaa gatcccgaag accggccgtt tcgaggccca
      541 cggcatcccc ggtagcgccc agcgcctgac cgacgaggtc gccccgaacg acctgggctc
      601 gctcgagcgc aagctggcgg ccaaccgcct gcgcggcggc accgcctgcg tgctgatcga
      661 gccggtcggc cccgagtcgg gcacgcggcc cgtcgccttc gactacaacc agggcgtgcg
      721 cgcgctctgc gacaagtacg gcgccctcct cgtgttcgac gaggtggtca ccgcgttccg
      781 catcggcctc gagggcgccc agggctactt cggcgtacgg cccgacctga ccaccttcgg
      841 caagatagtc gccggcggct acccgtcggc gggtggcctt ggcgggcgca aggacctgat
      901 cctgatgatg gccgcgggcc tcgagagcgg caagaagcgc gcttacgtcg gcggcaccat
      961 ggccgcgaac cccctgtccg cggcggccgg ctactttacg atcaaggaga tcgagaagcg
     1021 cgacgcctgc gccgtagccg ggcgtgccgg cgatcggctc acggccggcc tcaagaagct
     1081 gatagccgaa cgcggcctgc ccttcgtcgc ctacaaccag ggctccatct gccacctcga
     1141 gacggtcggc gcgatgttca tcaagatgga ctaccttaag atcgtgcgcg tcctcaagga
     1201 aattaagatc cgcaagaaga tgatggagac ctacggcgcg gcctacgcgg ccgagggcat
     1261 cgtgacgctc gcgggtagcc gcatgtacac gagcggcgcg gataccgacg ccgtgatcga
     1321 cgacgcgctc gccaggttcg gccgggtctt cgacaacgtc gacgtcgagg cgatgaaccg
     1381 cggggccgcg gaatgaccga ggccgaggcg agggacggtc cgggctgccg gcg
//
LOCUS       DAOPHV010000695         1428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_132594_length_1428_cov_4.060452, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000695 DAOPHV010000000
VERSION     DAOPHV010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132594_length_1428_cov_4.060452"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="PLQ29_13460"
     CDS             <1..995
                     /locus_tag="PLQ29_13460"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG87701.1"
                     /translation="SGSGVAGLRGIPAVAGMIGRPLLGLAKADILAYLDAEGLDYRVD
                     STNDSDAYLRNRVRHRLVPAAMDVFPSLRASLATVAEKASLDEEALSALADALFGLGD
                     DGARADWLEAGRYDAAPEAVRIRALYSLAMPLFEGAGATRLPWRLVRAAASSARREGV
                     LASGAGVLFSRNGGRIEARQALGPHLSRLSAPAGADRADSGFSLIANGPGSYRIGKGG
                     GCTIYSCGQAPGLRADAFSWPLCVRSRRPGDRLATRGGAKRVDAILSELGLSGASREA
                     TPVVEDALGLVAVLASSVGLCDVYRRNDALVGEPVGGYLVVELKGVMYIDAVRR"
     gene            979..>1428
                     /locus_tag="PLQ29_13465"
     CDS             979..>1428
                     /locus_tag="PLQ29_13465"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HPG87702.1"
                     /translation="MQFDDKNTPRTPRTPNKPDARPPRNARVSLILFIALLAASVGLF
                     MFDGAKTSPSIPYSTFLTHLGEGNVDSVRIVDQRLIEGTLRGKDGSSQEFSSVIPYVD
                     ASLLEQLKARGVRVEGSSARMGPLSVLAELFPWFFGFFLIWMVFRQSQ"
BASE COUNT          211 a          475 c          479 g          263 t
ORIGIN      
        1 gctccggctc aggcgtcgca ggcctgcgcg gtatccccgc cgttgccggg atgatcggcc
       61 gccccctcct cggcctggcc aaggccgaca tccttgcgta cctcgacgcc gagggactgg
      121 attaccgcgt cgactcgacc aacgactccg atgcatacct gcgcaaccgg gtcaggcata
      181 ggctcgtgcc cgctgcgatg gacgtcttcc cgtcgctgcg cgcgtcgttg gctacggtcg
      241 ccgagaaggc cagcctcgat gaggaagccc tctcggctct ggccgacgct ttgttcgggt
      301 taggagacga tggcgcccgc gccgattggc tcgaagcggg tcgctacgac gccgcgccgg
      361 aggccgtcag gatacgagcg ctctactcgc tggccatgcc gctgttcgag ggcgcaggag
      421 cgacgagact gccgtggcgg ctcgtccggg cggccgcgtc gtccgctagg cgcgaagggg
      481 tgctggcttc cggggccggc gtattgttct cgcggaacgg cggcaggatc gaggcccgac
      541 aggccctcgg cccgcacctg tcgcgcctgt ccgccccggc cggtgccgac agggccgact
      601 ccggcttcag cctcatcgcc aacggtcccg gatcgtatcg aataggcaaa ggcggcggct
      661 gtacgatata ctcgtgcggg caagcgcctg gattgcgcgc ggacgccttc tcgtggccgc
      721 tgtgcgtccg ctccaggcgg cccggcgatc gtctagccac gcggggcggc gccaagaggg
      781 tcgacgccat cctatccgag ctcggcttga gcggggcttc gcgcgaggct acgcccgtcg
      841 tggaggacgc cttgggcctg gtcgccgtcc tcgcctcgag tgttggcctg tgcgacgtct
      901 ataggcggaa tgatgccctg gtcggtgaac cggtcggcgg ctacctggta gtggagttga
      961 aaggagtcat gtacatcgat gcagttcgac gataagaata cgccgaggac gccgaggacg
     1021 ccgaataagc cggatgcgag gccgcctcgc aatgctcgcg tctcccttat actattcatt
     1081 gccttgctcg cggcctcggt cggtctgttc atgttcgacg gcgccaagac gtcgccgagc
     1141 atcccgtact cgacgttcct cacccatctc ggggagggca acgtcgattc tgtccgcatc
     1201 gtcgatcagc gcctcataga aggcacgcta cgcggcaagg acggctcgtc ccaggaattc
     1261 tcgagcgtca tcccctacgt cgacgctagc ctcctcgaac agctcaaggc gcgcggggtc
     1321 agagtcgagg gctcgtctgc caggatgggg ccgctcagcg tcctcgcgga gctattcccg
     1381 tggttcttcg gcttcttcct gatctggatg gtgttcagac agtcccag
//
LOCUS       DAOPHV010000696         1425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_133004_length_1425_cov_3.799270, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000696 DAOPHV010000000
VERSION     DAOPHV010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133004_length_1425_cov_3.799270"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1425
                     /locus_tag="PLQ29_13470"
     CDS             <1..>1425
                     /locus_tag="PLQ29_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPG87703.1"
                     /translation="PMTSGVFPVGTGLMRVIPIEKAIMGETRRASYEEISKYLEENDL
                     FTVSNCSCRSTREIMGEGCGHLKEDMCIQMGHAAEYYIRTKRGRQITREEAYEILKRA
                     EENGLMHQIPNIDGGGKTHAICNCCGCGCLSLRTAEMFHNTNMVRSNYVAKVDAEKCM
                     ACGECVENCPVGALKLGQKIHTKKPLPKLKKPDLPEFTDWTPEHWNPDYRTNREVSFA
                     TGTSPCKAECPAHIGVQGYVKLASEGKYAEALELIKRENPFPAVCGRVCSKRCEAGCT
                     RNGLDEPVAVDDIKKFIADQDLNATRRFVPNKRHDYSDKKIAIVGAGPAGLSCAYYLA
                     LDGYSVTVFEKRAMLGGMLTLGIPSFRLEKDVINAEIDIIRELGVEFRTGVEVGVDVT
                     LAELRKQGYTGFYLAIGAQAGRRMGIEGEDAEGVMTGVDFMRSVNLGEKVSLPGKVVV
                     IGGGNVAIDVARTAARVGAATVGM"
BASE COUNT          290 a          457 c          462 g          216 t
ORIGIN      
        1 gcccatgacc tccggcgtct tccccgtcgg cacgggcctc atgcgcgtca tcccgatcga
       61 gaaggccatc atgggcgaga ccaggcgggc ctcctacgag gaaatatcca agtacctgga
      121 ggagaacgac ctcttcaccg tgtcgaactg ctcatgccgc tccacgcgcg agatcatggg
      181 cgagggctgc ggccacctga aggaagacat gtgcatccag atgggccacg ccgcggagta
      241 ctacatacgc acgaaacgcg gccgtcagat cacccgagag gaagcctacg agatactcaa
      301 gcgggccgag gaaaacggcc tgatgcacca gatacccaac atcgacggcg gaggcaagac
      361 ccacgccatc tgcaactgct gcggctgcgg ctgtctatcc ctgcgtaccg ctgaaatgtt
      421 ccacaacacc aacatggtac gctccaacta tgtggccaag gtcgacgccg agaagtgcat
      481 ggcctgcggc gagtgcgtgg agaactgccc cgtcggcgcc ctcaagctcg gccagaagat
      541 ccataccaag aagccgctac cgaagctcaa gaagccggac ctgcccgagt tcaccgactg
      601 gacgccggag cactggaatc ccgactaccg caccaaccgc gaggtatcct tcgcgaccgg
      661 cacgagtccc tgcaaggccg agtgcccggc gcatatcggt gttcaaggct acgtcaagtt
      721 ggcgtccgag ggtaagtacg ccgaggcgct ggagctgatc aagcgcgaga acccgttccc
      781 ggccgtctgc ggccgcgtct gctccaagcg ctgcgaggcg ggttgcaccc gtaacggcct
      841 ggacgagccg gtggccgtgg acgacatcaa gaagttcata gccgatcaag atctcaacgc
      901 aacgcgccgc ttcgtgccga ataagcgcca cgactatagc gacaagaaga tagctatcgt
      961 gggcgccggc cccgcgggcc tgtcctgcgc ctactacctg gccctggacg gctactcggt
     1021 caccgtgttc gagaagcgcg cgatgctcgg cggcatgctg acgctgggca tcccgagttt
     1081 ccgcctggag aaggacgtca tcaacgcgga gatcgatatc atccgcgagc tgggcgtgga
     1141 gttccggacc ggcgtcgagg tcggtgtcga cgtgacgctg gcggagctgc gcaagcaggg
     1201 ctataccggc ttctacctgg ccataggtgc gcaggccggc cgcaggatgg gcatcgaggg
     1261 cgaggacgcc gagggggtga tgaccggcgt cgacttcatg cgttcggtca acctgggcga
     1321 gaaggtcagc ctgccgggca aggtcgtcgt cataggcggc ggcaacgtgg ccatcgacgt
     1381 ggcgcgcacc gcggcccgcg tcggcgccgc gaccgtcggc atgta
//
LOCUS       DAOPHV010000697         1424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_133156_length_1424_cov_3.575603, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000697 DAOPHV010000000
VERSION     DAOPHV010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133156_length_1424_cov_3.575603"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..>1424)
                     /locus_tag="PLQ29_13475"
     CDS             complement(255..>1424)
                     /locus_tag="PLQ29_13475"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87704.1"
                     /translation="PPPEPIAADAGQVAAEAGRGAAEAVAADVAAWSETDRLMVELSF
                     ASYAGFWLDDPELAAAEVSAFLAGFLPGQELVWGPAAHSPDTLVFTHPTDALVFIIRD
                     AASGDYTVVFRGTNPISGYEWLFQDFMIDGQTPWNAVTVFVGEPRTGSGGAPDEALVS
                     EGTATALSLRLALTPGQGFPGAGTSLADALVGLAGSADGTSRLRFTGHSLGGLLAPVA
                     ALWLSERLDDALEGAARPSLSVHGIASPTPGNAAFAAYLDSRLPDYDRYANSLDVAAL
                     AWAEDSMAILPGLYKPTIRMTSILKQLYRFCSRLARGKGYAHAGELVPVPSSVVPVYG
                     DVYSLQMIYQHSVAYLHMLEPERREAIVAGVIGPLAELVRIPGAEPFDVRKLFAD"
BASE COUNT          245 a          484 c          496 g          199 t
ORIGIN      
        1 agaagctgat cttccagagc gcgtggcggg acgaagccgg agaggtcgcc ggcctcgtcg
       61 aactgtcgct cgtcctgccg gccgatatgc cccactacgt gcgcggctga gacgccggca
      121 gcgccgtggc gacctcccag gtctcgttgc cgccggatgg gttcccgacg cggccgaaga
      181 ccagcacgaa ccgcccgcgc ggtatctccg cgaaccggcc gcgtagctga tggaatcgcc
      241 gcaacccatg cagctcagtc ggcgaacagc ttccgcacgt cgaacggctc tgctccggga
      301 atgcgcacca gctcggcgag cgggccgatg actcccgcga cgatggcctc gcgcctctcc
      361 ggttcgagca tgtgcagata ggccaccgaa tgctggtaga tcatctgtag gctgtataca
      421 tcgccataga ccggcacgac cgacgacggc accggtacga gctcgcctgc gtgagcgtag
      481 cccttgcccc tcgccagccg cgaacagaac cggtacagct gcttcaggat cgaggtcatg
      541 cgtatcgtcg gcttgtacag gcccggaagg atcgccatcg agtcctccgc ccaggccagg
      601 gccgcgacgt cgagactgtt ggcgtagcgg tcgtagtcgg gcaggcgcga gtcgaggtag
      661 gcggcgaagg cggcgtttcc cggggtcggc gaggcgatac catggaccga tagggacgga
      721 cgagccgcgc cctcgagggc atcgtcgagc cgctcggaca gccatagggc agcgaccggc
      781 gccaggaggc ccccgaggct gtgtccggtg aagcggaggc ggctcgtccc gtcggcagac
      841 cctgccagcc cgacgagggc gtccgcgagg ctggtcccgg cgccgggaaa accctgcccc
      901 ggcgtcaggg cgagccgcag gctcagagcg gtcgccgtac cctcggagac gagcgcctcg
      961 tcgggggctc cgccgctacc ggtgcgcggc tccccgacaa agacggtcac ggcattccac
     1021 ggcgtctggc cgtctatcat gaagtcctgg aacagccact cgtaacctga aataggattg
     1081 gtacccctga agacgaccgt atagtcgccg gaggccgcgt cgcggataat aaagacgagg
     1141 gcgtcggtcg gatgggtgaa gacgagcgtg tcggggctat gcgcggccgg gccccatacc
     1201 agttcctggc ccggtaggaa gcccgccaga aaagccgata cctccgcggc cgccagctcc
     1261 gggtcgtcca gccagaaacc ggcgtaggag gcgaaggaca gctcgaccat cagcctgtcg
     1321 gtctcggacc aggcggcgac gtcggccgct actgcttcag cggcgccccg accggcctcc
     1381 gcggcgacct gcccagcgtc ggcggctatc ggctcgggcg gcgg
//
LOCUS       DAOPHV010000698         1424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_133167_length_1424_cov_3.311907, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000698 DAOPHV010000000
VERSION     DAOPHV010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133167_length_1424_cov_3.311907"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PLQ29_13480"
     CDS             complement(<1..938)
                     /locus_tag="PLQ29_13480"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87705.1"
                     /translation="MLKTHLILGTYNHLPEGLDETEFERTYQSCYRPFLSTLNRFPEI
                     QAALYYSGSLLKRFEARHPEYLMLLEEMSARRQIELIGGGFYAPILPLIPNSDRLGQT
                     ELMTTYLRKSFGKRPKGCWLPEYAWEPWLASTLQSSGMDYTFLLDRQFKEAEGRDDAA
                     LSPVVTEDQGRCLTVLPVRDCSFSFGKPLGLVEAAEAAGPSQLTVLMVAGERIRPLWE
                     ESGLESPDLYMERSLAWFRKNSLEAETTTPSRYLKSKRVARKLYFGGSASERFMKASA
                     PDGAEHGCGSLRRSIIRYAASSALYSRMYYVHLLIG"
     gene            complement(1022..>1424)
                     /locus_tag="PLQ29_13485"
     CDS             complement(1022..>1424)
                     /locus_tag="PLQ29_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87706.1"
                     /translation="LLSPWGLESLAPILFSALLFGAFAAIKAIGTALGRPSRPLHDET
                     LFQPTLVLYAVALSIGSRFSSTGLLLLGGAAAALGYIAATGFLEAIMERLELERVPEP
                     FRGVPLRFLSAALMALAFAGVDAGFFSRLSL"
BASE COUNT          290 a          437 c          457 g          240 t
ORIGIN      
        1 tgcccgatga ggagatggac gtagtacatc ctcgaataaa gcgcggagga agccgcgtat
       61 cggatgatgg agcgccgcag gctaccgcag ccatgctcgg cgccgtcggg agcgctcgcc
      121 ttcatgaatc gctctgaggc cgagccgccg aagtacagct tgcgcgccac gcgcttggac
      181 ttcagatagc gcgatggcgt ggtggtctcc gcctcgaggg agttctttcg gaaccaggcc
      241 agggaacgct ccatgtacag gtccggcgac tcgaggcccg attcctccca gagcggccgt
      301 atcctctcgc cggcgaccat cagcacagtc agctggctcg ggccagccgc ctcggccgcc
      361 tctaccaggc ctaagggttt gccgaagctg aagctgcagt cgcgcaccgg tagcacggtc
      421 aggcatcggc cctggtcctc ggtgacgacc gggctcaggg ccgcgtcgtc acgcccctcg
      481 gcctccttga attgccggtc cagaaggaag gtatagtcca tgcccgacga ctgcaacgtg
      541 gaggccagcc agggctccca ggcgtattcg ggcagccaac agcccttggg acgcttaccg
      601 aaactcttac gcaggtaggt ggtcatcagc tcggtctggc caagccgatc agagttgggg
      661 attaaaggca ggatgggcgc gtagaaaccg ccgccgatca gctcgatttg acgcctggcg
      721 ctcatctcct cgagaagcat caggtattct ggatgcctgg cctcgaaacg cttcagcaag
      781 ctgcccgaat agtacagggc cgcctggatc tctggaaaac ggttcaacgt cgataggaac
      841 ggcctatagc acgactggta ggttctctcg aactccgtct cgtcgagccc ttccgggaga
      901 tgattgtacg tccctagtat gaggtgcgtc ttaagcattc cggaacatcc tttataatca
      961 tgaacgatca ttatatctca gttcgtggcg gcgcgataga ctgaaaggcc cggcggccct
     1021 ctcagagaga aagcctcgag aagaaaccgg cgtcgacccc ggcgaaggcg agcgccatca
     1081 gcgcggcact caggaagcgg agcggtacac ccctgaaagg ctcgggcacg cgttccagct
     1141 ctagccgttc catgatggcc tcgaggaagc ccgtcgccgc gatatagccg agggccgccg
     1201 ctgcgccacc gagtaacaga agcccggtgg acgagaaccg cgagcctatg gagagggcca
     1261 cggcgtagag caccagcgtc ggctggaaga gggtttcgtc gtggagcggc cgtgatggcc
     1321 ttccgagagc ggtccctatc gccttgatcg cggcgaacgc cccgaacagg agggccgaga
     1381 acaggatcgg cgccagagat tcgaggcccc agggcgacag cagg
//
LOCUS       DAOPHV010000699         1422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_133462_length_1422_cov_3.629846, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000699 DAOPHV010000000
VERSION     DAOPHV010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133462_length_1422_cov_3.629846"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLQ29_13490"
     CDS             complement(<1..235)
                     /locus_tag="PLQ29_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87707.1"
                     /translation="MNKTKRFIIVLVVLAVAFAFLFPSLQWYAMTPKEDQALALGSRE
                     QIREYAWRMARADLAEIERLVAASVVHRDESGSR"
     gene            complement(309..677)
                     /gene="yajC"
                     /locus_tag="PLQ29_13495"
     CDS             complement(309..677)
                     /gene="yajC"
                     /locus_tag="PLQ29_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPG87708.1"
                     /translation="MDSLFSGLSLFQAAPANSTGSMISTVVMFGAVFAIFYFLIIRPQ
                     NKKQKEAQKMIAAVKKGDKVVTIGGVHGTVSAVKEKTVVIKVDDSAKIEFTKSAIASV
                     DARGDEAPAEAEDSKKEASK"
     gene            complement(950..>1422)
                     /locus_tag="PLQ29_13500"
     CDS             complement(950..>1422)
                     /locus_tag="PLQ29_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPG87709.1"
                     /translation="GYRRVVASPIPVDVVEKGVIHELLDSGKVVIAVGGGGVPVCRLA
                     DGTLEGVDAVIDKDRASALLAGLIKADELLILTGVDKVAVNYKKPDQKTLDTLTVAEA
                     ERYMAEGQFPKGSMGPKIEAAIDFVKRGGKTVVITSLERAQDAVDGNAGTRVVK"
BASE COUNT          252 a          438 c          460 g          272 t
ORIGIN      
        1 tgcgggagcc gctctcgtcg cggtgcacga cggaggcggc cacgaggcgc tcgatctccg
       61 ccaggtccgc gcgcgccatg cgccacgcgt actcgcgtat ctgttcgcgg gagccgagcg
      121 ccagcgcctg gtcttccttc ggggtcatgg cgtaccactg cagcgagggg aagaggaagg
      181 cgaacgcgac ggccaacact acgagcacga tgatgaatct tttggttttg ttcatgcgtt
      241 gactccggtg gtgtcgtaag ggggtaggac tagcggcccc gaccgcttcg gtcggggccg
      301 ccgggacgtt acttcgaggc ttctttcttg gagtcctcgg cctcggccgg ggcctcgtcg
      361 ccgcgcgcgt caacggacgc tatcgccgac ttggtgaact cgatcttggc cgagtcgtcg
      421 accttgatca cgacggtctt ttccttgacg gcgctgaccg tgccgtgaac gccgccgatg
      481 gtgacgacct tgtcgccctt cttgacggcg gcgatcatct tctgggcttc cttctgcttc
      541 ttgttctggg ggcggatgat aaggaagtag aagatggcga agacggcgcc gaacatgacg
      601 acggtcgaga tcatcgagcc ggtcgaattg gcgggggcgg cctgaaacag gctcaggccg
      661 gaaaacaaac tatccatgtc agtgtcctca atctatagaa aggattgacc gcaaaggtac
      721 catagaagat atgcaccgtc aagtttgccg agaagcggga taactacaaa aacggcgaac
      781 cacggaggca cggagacacg gagaggaaag ggcgagagcg gattcggaaa tgggcaaagc
      841 accctggcgt tactactcta ccaggatatc gcccacctat cccacacgat cgccttcctc
      901 cgtgcctccg tggctccgtg gccggattcc tcgttataag cttgacgcct tacttcacga
      961 cgcgggtgcc ggcgttgccg tccacggcgt cctgggcccg ttcgaggctg gtgatgacca
     1021 ccgtcttgcc gccgcgcttg acgaagtcga tggcggcctc gatcttggga cccatcgagc
     1081 ccttggggaa ctggccctcg gccatgtagc gctcggcttc cgccacggtg agcgtatcga
     1141 gcgtcttctg gtcgggcttc ttgtagttga cggccacctt gtcgacgccg gtcaggatca
     1201 ggagctcgtc ggccttgatc agcccggcga gcagggcgct cgcgcggtcc ttgtcgatga
     1261 cggcgtccac accctcgagc gtgccgtcgg ccagcctgca gaccggcacg ccgccgccgc
     1321 ctacggcgat gacgaccttg ccggagtcga gcaactcgtg gatcacgccc ttctcgacca
     1381 cgtcgacggg gatcggagaa gcgacgacac ggcggtagcc ac
//
LOCUS       DAOPHV010000700         1420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_133728_length_1420_cov_4.955311, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000700 DAOPHV010000000
VERSION     DAOPHV010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133728_length_1420_cov_4.955311"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..982
                     /locus_tag="PLQ29_13505"
     CDS             125..982
                     /locus_tag="PLQ29_13505"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPG87710.1"
                     /translation="MRVAILGAGKMGAWLIRSLAAAGHELAAYDPSPGRLGAATAGVV
                     GSATGKPVEAADDVRALAAFGPELLVNCAPLGLTTEAFDAVTPHLDEACALSDIASVK
                     TGLASWYASSGRPFSSSHPMFGPTYADGSNLSEQSAVIIAESSDDGKAFWKSFYGSFG
                     IRVFEESFDGHDRTVAYSLGTPFASTMVFAACMKRLDAPGTNFRKHLDIARGLLAEDD
                     RLLAEIMFNPYTIRRLELINSQLAYLTHIIKDRDHEEMGKFLDKLRGNLGEAPDGPAG
                     YAAAPPTRP"
     gene            complement(972..>1420)
                     /locus_tag="PLQ29_13510"
     CDS             complement(972..>1420)
                     /locus_tag="PLQ29_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87711.1"
                     /translation="EAAGAELERVPGGYRVGGGALLGFDFDATDCPDLFPPLVALASR
                     CRGTSRLTGAARLRGKESDRAAALSEEFGRLGLEVAASGDELTVVGLGDGRLGAGTVS
                     SRGDHRIAMAAAVAALAGSGPVAIDAAECVSKSWPGFYGALDSIRA"
BASE COUNT          206 a          542 c          477 g          195 t
ORIGIN      
        1 gccatcttca tcgacttcgc tggctccgac cgcgacgaag cggtgatcaa ggcgctcgac
       61 cgcgccggcg gcctgtcgcg aggcttcagg ttgctcggct gctacgccga gaggaggctg
      121 tagcgtgcgc gtcgcgatac tcggcgccgg caagatgggc gcctggctga tacgttccct
      181 cgcggccgcc ggccacgagc tggcggccta cgacccgtca cccggtcgcc tgggggcggc
      241 gaccgccggc gtcgtcggct cggccaccgg gaagcccgtc gaggccgccg acgacgtccg
      301 cgccctggcg gccttcgggc cggagctgct cgtcaactgc gcgccgctcg gcctgacgac
      361 ggaggccttc gacgccgtga cgccccatct ggacgaggcc tgcgcgctgt ccgacatagc
      421 ctcggtcaag accggcctcg cgtcatggta cgcctcgtcg ggccggccct tctcgtcgtc
      481 ccacccgatg ttcggcccga cctacgccga cggctcgaac ctgagcgagc agagcgcggt
      541 gatcatcgcc gagtcgagcg acgacggcaa agccttctgg aagtcgttct acggatcctt
      601 cggcatccgg gtcttcgagg agagcttcga cgggcacgac cggacggtcg cctactcgct
      661 gggcacgccc ttcgcctcga cgatggtctt cgccgcctgc atgaagcgcc tcgacgcgcc
      721 cggcaccaac ttccgcaagc acctggacat cgcgcgcggc ctactcgctg aggacgaccg
      781 cctgctcgcc gagatcatgt tcaacccgta cacgatacgg cgcctggagc tgatcaactc
      841 ccagctggcc tacctgaccc atatcatcaa ggaccgcgac cacgaggaga tgggcaagtt
      901 cctggacaag ctgcgaggca acctcggcga ggctcccgac ggcccggccg ggtacgccgc
      961 cgcgccgccg actaggccct gatcgagtcg agcgccccgt agaagcccgg ccagctcttg
     1021 gacacgcact cggcggcgtc gatggccacc gggcccgagc cggcgagggc ggccacggcg
     1081 gcggccatcg ctatacggtg gtcgcctcgc gacgataccg tcccggcgcc caggcggccg
     1141 tcgcccaggc cgaccacggt cagctcgtcg ccgctcgccg ccacctcgag cccgagccgg
     1201 ccgaactcct ccgacagcgc ggcggcccgg tcgctctcct tgccgcgcag cctggcggcc
     1261 ccggtcagcc ggctcgtccc tcggcaacgc gaggccaggg cgacgagcgg cgggaacagg
     1321 tcggggcaat cggtcgcgtc gaagtcgaag ccgaggaggg cgccgccgcc gacccgatag
     1381 cccccgggta cgcgctcgag ctctgccccg gccgcctcga
//
LOCUS       DAOPHV010000701         1420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_133738_length_1420_cov_4.192674, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000701 DAOPHV010000000
VERSION     DAOPHV010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133738_length_1420_cov_4.192674"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PLQ29_13515"
     CDS             complement(<1..833)
                     /locus_tag="PLQ29_13515"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPG87712.1"
                     /translation="MPEEKRNSSLARINLLLLGFIALILALAALKLSASVLLPLIIAI
                     LLTFVLEPVVVLLEKIRIPRALGAIVVVVLIAAGVYMVGAILFGSLKTILALYPKYEL
                     RFNHLYSLFANLFNLPYDEHISLFQNLWGQLGVRERVQALALTTSESFMGFATDTVMV
                     VLFVVFLLLEIGHFRKRIELAFEGFMSTRIQRIVADVIKQVARYLSTKFFISLATGVL
                     VSVLLGWIGMDLPIIWGVISFILNFIPNIGSIAAGAGVTLFAVVQFWPQPGPILAAFA
                     T"
     gene            complement(845..>1420)
                     /locus_tag="PLQ29_13520"
     CDS             complement(845..>1420)
                     /locus_tag="PLQ29_13520"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPG87713.1"
                     /translation="VAPPRSPAPDEYLRSIEPAPVPSTVPAPTPDAAAASGASDIIVV
                     YGDKPAPTALPGIAPSASAQATTSQPVPKPYAPAPASPVASKPAAAQSSSSAAAKPAA
                     KAPVTVAEYWIQAAAFTSRTRADDLQRELAAKGLSTLITVKDLDGVTWYRVRIGPYTN
                     EREAKGWLEQIRVVAGCADAYVSKQTVQRSS"
BASE COUNT          261 a          410 c          506 g          243 t
ORIGIN      
        1 atcgtcgcga aggccgccag aatcggtccc ggctgcggcc agaactgcac gaccgcgaag
       61 agcgtgactc cggctccggc ggctattgag cctatgttcg ggataaaatt cagtatgaac
      121 gaaatgacgc cccagatgat cgggagatcc atgcctatcc agcccagtag gacgctgacg
      181 agcacgccgg tagccaggct gatgaagaac ttggtactca ggtagcgggc cacctgcttg
      241 atcacatcgg ccacgatgcg ctgaatcctc gtcgacatga agccctcgaa ggccagctcg
      301 atgcgcttcc tgaagtggcc tatctcgaga agcaggaaca ccacgaacag cacgaccatc
      361 acggtatcgg tcgcgaagcc catgaacgac tcggaggtgg tcaaggcgag cgcctgtacc
      421 cgctcgcgga cgcccagctg gccccagagg ttctggaaca ggctgatatg ctcgtcgtag
      481 ggcaggttga acaggttcgc gaagagcgag tataggtggt tgaagcgtag ctcgtacttc
      541 ggatacaagg cgaggatggt cttgaggcta ccgaacagga tggccccgac catatagacg
      601 ccggcggcga tcagcacgac gacgacgatg gcgcctagcg cccgcggtat ccgtatcttc
      661 tccaggagga cgacgaccgg ctctagtacg aaggtcagga ggatggcgat gatgagcggt
      721 agcagtaccg atgcgctgag cttgagggcc gccagggcca ggatcagagc gatgaagccg
      781 agcaggagca ggttgatcct ggccaggctg gaattgcgct tctcttcggg cacgattgta
      841 tcctttagct cgagcgctgg acggtctgtt tcgagacgta ggcgtcggcg caaccggcga
      901 ctacgcgtat ctgctcgagc cagcccttcg cctcgcgctc gttcgtatac gggccgatgc
      961 gtacgcggta ccaggttacc ccgtcgaggt ccttgacggt gatcagcgtg gacaggccct
     1021 tggcggccag ctcgcgctgt aggtcgtcgg cgcgggtccg gctcgtgaac gcggcggcct
     1081 gtatccagta ttcggctacc gtgaccggag ccttggcggc aggcttggca gccgcggagc
     1141 tggacgactg ggctgcggcg ggcttcgagg cgaccggcga ggctggcgca ggcgcgtacg
     1201 gcttcggtac gggctggctc gtcgtcgcct gagcgctcgc gctcggggcg atgccgggca
     1261 gggccgtcgg agcgggcttg tcgccgtaga cgacgatgat atctgaggct ccgctcgcgg
     1321 cggccgcgtc gggcgtcggc gccggaacgg tggacggtac gggggcgggt tcgatggagc
     1381 gtaggtactc atcgggcgcg ggcgagcgcg gcggggcgac
//
LOCUS       DAOPHV010000702         1420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_133748_length_1420_cov_3.738462, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000702 DAOPHV010000000
VERSION     DAOPHV010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133748_length_1420_cov_3.738462"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1420)
                     /locus_tag="PLQ29_13525"
     CDS             complement(<1..>1420)
                     /locus_tag="PLQ29_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HPG87714.1"
                     /translation="AGAVKSGKTMKASVSDFMEMEKERGISISTSVMGFEYGDRKINL
                     LDTPGHADFSEDTYRGLSAVDSALLVIDSVKGVEDRTRKLCEVCRMRHTPIATFMNKL
                     DREGKDPMELLDEAERELAIGVRPVTWPVGQGRSFKGVYDLVQKRLLLFFPGESQRPE
                     DVVTIRDLADPLLEQKVGPSLAAKLRDDVALLSGVYPAFDAAEYLAGKATPVFFGSAI
                     NNFGIRELLDAMTSLAPAPTPKRCDERVVHPGEDKFSGFVFKIHANMNPRHRDRVAFM
                     RVCSGTFERNKTYYHVRSGKPFRTANPTAFMAQDSEIVNEAWPGDIIGIHDTGTFKIG
                     DTVTEGEAINFKGIPSFAPQIFRYIVNADPLKEKQYHKGLDQLAEEGVVQIFSKPHQP
                     NVRVLGVVGQLQLEVLQYRMEHEYGAKCSYRPIDLSIAHWITSDDRKALEAFADEHSR
                     RILVDIRDSFVFMSESEWQFER"
BASE COUNT          221 a          437 c          487 g          275 t
ORIGIN      
        1 atgcgctcga actgccactc ggactccgac atgaacacga agctatcgcg tatgtcgacg
       61 agtatccgcc gcgagtgctc gtcggcgaag gcctcgagcg ctttgcggtc gtccgacgtg
      121 atccagtggg ctatggaaag gtcgatggga cgatagctac acttggcgcc gtactcgtgc
      181 tccatgcggt actgcaatac ctcgagctga agctgaccga ccacgccgag tacgcgcacg
      241 ttcggctggt gcggcttcga gaagatctgc accacgcctt cctcggccag ctggtccagg
      301 cccttgtggt actgcttctc cttgagcggg tcggcgttga cgatatagcg gaagatctgc
      361 ggcgcgaagc tcgggatgcc cttgaagttg atcgcctcgc cctcggtgac ggtgtcgcct
      421 atcttgaagg tgccggtgtc gtggatgccg atgatgtcgc cgggccaggc ctcgttgacg
      481 atctcgctgt cctgggccat gaacgcggtc gggttggcgg tcctgaaggg cttgccggag
      541 cgcacgtggt agtaggtctt gttgcgctcg aaggtgcccg agcagacgcg catgaaggcg
      601 acccggtcgc ggtgcctcgg gttcatgttg gcgtggatct tgaacacgaa gccggagaat
      661 ttatcctcgc cggggtggac cacccgctcg tcgcagcgct tcggggtggg ggccggggcg
      721 agggaggtca tcgcgtcgag cagctcgcgg atgccgaagt tgttgatggc cgagccgaag
      781 aagaccggcg tcgccttgcc cgcgaggtac tcggccgcgt cgaaggccgg atagacgccg
      841 gacaggaggg ccacgtcgtc gcgtagcttg gccgccagcg acgggccgac cttctgttcg
      901 agcagcgggt ccgcgaggtc gcgtatcgtc accacgtcct cggggcgctg gctctcgccg
      961 gggaagaaca ggagcaggcg cttctggacg aggtcgtaga cgcccttgaa gctccggccc
     1021 tgcccgactg gccaggtgac cggcctgacg cctatggcca gctcgcgctc cgcctcgtcg
     1081 agcagctcca tcgggtcctt gccctcgcgg tcgagcttgt tcatgaaggt ggctatgggc
     1141 gtatgccgca tccggcagac ctcgcacagc ttacgcgtgc ggtcctcgac gcccttgacc
     1201 gagtcgatca cgagtagcgc gctgtcgacc gccgacaggc cccggtaggt gtcctccgag
     1261 aagtcggcgt ggccgggcgt gtcgaggaga ttgatcttgc ggtcgccgta ctcgaagccc
     1321 atcaccgagg tgctgatcga tatgccgcgt tccttctcca tctccatgaa gtcggagacg
     1381 gaggccttca tcgtcttgcc gctcttgacc gcgccggcga
//
LOCUS       DAOPHV010000703         1416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_134303_length_1416_cov_5.742101, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000703 DAOPHV010000000
VERSION     DAOPHV010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134303_length_1416_cov_5.742101"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..444
                     /locus_tag="PLQ29_13530"
     CDS             193..444
                     /locus_tag="PLQ29_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87715.1"
                     /translation="MKGISVAKRTNYSFEKRQKDLAKKKKREDKLKKKQERKASGVQT
                     IVQIDEYGNVVEIEVDADGNPIVDSDDDDDDDDDSDDED"
     gene            complement(487..714)
                     /locus_tag="PLQ29_13535"
     CDS             complement(487..714)
                     /locus_tag="PLQ29_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HPG87716.1"
                     /translation="MAVSVYSTPSCSYCRLVKDWLKARNVRFDDYNVAQDQRRAEEMV
                     RKSGQMGVPVTDVNGRVIVGFNTAELERALR"
     gene            811..>1416
                     /locus_tag="PLQ29_13540"
     CDS             811..>1416
                     /locus_tag="PLQ29_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87717.1"
                     /translation="MSHTGHGGLDPLKAGGLAPIAIRAAIAAAWLLSAGLDAWPSPQR
                     AASAIGALELSNVDGLDCKAAIATAAAGLRFSYRVPVPASDDPAEVGFTMTLPYLLVG
                     SARPVGLAAFVEAPASAIVTLLGRSGAPLEAAWPLAATRVGAALGDDYGAYAELPAFD
                     GGSAGDWAAYGFWAAPRGSPLSALASVVETEAEPGGLGWYDP"
BASE COUNT          258 a          466 c          449 g          243 t
ORIGIN      
        1 cgaccctgag caagagttcc ttgacctgat cctggttcat cgatggcctc cgccgcgcac
       61 tgtactacgg cgccgctcgg ggcggcaagc gactcagtat cgcgaccggc atagaccatg
      121 aggtaaaaaa ggtttaggat agtagctctc aggcgtcggt tcgaagcgcc gagccccaac
      181 catcgttcaa tcatgaaagg gatatccgtg gcgaaaagga ccaattattc gttcgagaag
      241 cgccagaagg atctggctaa gaagaagaag cgcgaggata agctcaagaa gaagcaggag
      301 cgcaaagcct ccggcgtcca gaccatcgta cagatagacg agtacggcaa cgtggtcgag
      361 atcgaggtgg acgcggacgg caatcccatc gtcgactcgg atgatgacga cgacgatgac
      421 gacgactcgg atgacgaaga ctagcgggag ccgtttcagc cggccaggct ccccacgggc
      481 cgacgtctag cgcagcgcgc gctccagttc ggccgtattg aatccgacga tgacgcgtcc
      541 gttgacgtcc gtcaccggca cgcccatctg tccggatttc ctgaccatct cctcggcccg
      601 cctctgatcc tgggccacgt tgtaatcgtc gaagcgtacg ttcctggcct tgagccagtc
      661 ctttaccagc ctgcagtacg agcagctcgg cgtcgagtat accgatacag ccatatagcg
      721 cctcctaata tatggaaaag tatatgtata gaataccgat agatacgccg gtcgtcaagc
      781 gaggcggctc cccgcgtcgt atgcgcccgt atgagtcata caggacacgg cggccttgat
      841 ccgctcaaag ccggagggct agctccgatc gcgatcaggg ccgcgatcgc cgcggcttgg
      901 ctcctctcgg ccggccttga cgcatggccc tctcctcagc gggccgcgtc ggcgatcggc
      961 gccctcgagc tgtcgaacgt cgacggcctc gactgcaagg ccgcgattgc cacggccgcc
     1021 gccgggttac ggttcagcta ccgcgttcct gttcccgcct ccgacgatcc ggccgaggtc
     1081 ggctttacca tgaccctacc gtacctgctc gtaggctcgg ccaggccagt ggggttggcc
     1141 gccttcgtcg aggcgccggc ctcggccatc gtgacgctgc tcggccgctc gggagcgccg
     1201 ctcgaggccg cttggcccct cgcggcgacg cgggtcggcg cggcgctcgg agacgactac
     1261 ggcgcctacg ccgagctgcc ggccttcgac ggcggctcgg ccggggactg ggccgcgtac
     1321 ggattctggg cggcgccccg aggctcgccg ctgtcggcgc tcgccagcgt cgtcgagacc
     1381 gaggccgagc ccggcggcct cggctggtac gacccc
//
LOCUS       DAOPHV010000704         1413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_134805_length_1413_cov_3.336524, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000704 DAOPHV010000000
VERSION     DAOPHV010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134805_length_1413_cov_3.336524"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="PLQ29_13545"
     CDS             complement(<1..729)
                     /locus_tag="PLQ29_13545"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HPG87718.1"
                     /translation="MGSQPTILMAMLECGFGHKNPALAVRDAIEEDYPGAFAVEVVDL
                     ARESGAIGADELLKRSWDFSLAFPPPARIGYLLVELAGERKEYLDVLFREFVVKGMAY
                     IDRYRPDLIFSTHPLCTYIAVKAREKLGLAFRLVTYVVDPFDGYSWWAEKGSDYTLVA
                     TDRSRDRLLRHGIPPEKIRVIGFPINKRFMRVEADRDEILDRLGLDPANTTLLVSAGG
                     QGIGKVFSYVEALARHELPLNVITV"
     gene            829..>1413
                     /locus_tag="PLQ29_13550"
     CDS             829..>1413
                     /locus_tag="PLQ29_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG87719.1"
                     /translation="MKEQLIVKNLPFIKILPSWAQELSYKYCSKTANLYIVHGNIRDF
                     LPHKMNEGEFNFVKIQDYVSEVLFGNRDIIVFWDRSSGVSFCSPEMQRDYIRAMKERF
                     PDTEDDELLSSDPAVAFRTLERYFLLNIPQKKRIVMIIDYAETVLPAGEVAKLDESDR
                     YCFVTLNRWSHDPVFTKGDVSVLLLSENLADLNPR"
BASE COUNT          269 a          456 c          432 g          256 t
ORIGIN      
        1 caccgtgatg acgttcagcg gcaactcgtg cctggcgagc gcctcgacgt acgagaagac
       61 ctttccgatg ccctgtccgc cggctgagac gagtagcgtc gtgttcgcgg gatccaggcc
      121 gagccggtcg agtatttcgt cgcggtcagc ttcgacgcgc atgaagcgct tattgatggg
      181 gaagcctatg acgcggatct tctcgggggg tatgccgtgg cgcaggagcc ggtcccgtga
      241 tcggtcggta gccaccaggg tatagtccga gcccttctcg gcccaccacg agtagccgtc
      301 gaaggggtcg acgacgtagg tgacgagcct gaacgccagg ccgagcttct cccgggcctt
      361 gaccgcgata taggtgcata gcgggtgggt cgagaagatc aggtctggtc ggtagcggtc
      421 gatataggcc atgcccttga cgacgaattc cctgaacagc acatcgaggt attctttgcg
      481 ctcgccggcc agctcgacga ggaggtagcc gatgcgcgcc ggcggcggga acgccagcga
      541 gaagtcccag gaacgcttga ggagctcgtc ggcgccgatc gcgccgctct ccctggccag
      601 gtcgaccacc tcgacggcga acgcgccggg atagtcctct tcgatggcgt cgcggacggc
      661 cagggcggga ttcttgtggc cgaagccgca ctcgagcatg gccatcagta tcgtaggctg
      721 gctacccata cggtaaggat actgccgccc gagcccctag tgaagcggaa agccgacgac
      781 acttgccccc gtggcccgcg atcgtacata ctcctccatg gagggcgcat gaaagagcag
      841 ctcatcgtca agaaccttcc ctttatcaag atactgccat cgtgggccca ggagctgtcg
      901 tacaagtact gctccaagac cgcgaacctt tatatcgtcc acggcaacat ccgcgacttc
      961 ctgccccaca agatgaacga gggcgagttc aacttcgtca agatccagga ctacgtctcc
     1021 gaggtgctgt tcggcaaccg cgacatcatc gtgttctggg accgctcctc cggcgtcagc
     1081 ttctgctcgc ccgagatgca gcgcgactat atacgcgcca tgaaggagcg cttcccggac
     1141 accgaggacg acgagctcct gtcgtcggac cccgccgtcg ccttcaggac cctcgagcgc
     1201 tacttcctgc tcaacatccc gcagaagaag cgtatcgtga tgatcatcga ctacgccgag
     1261 acggtcctgc ccgccggcga ggtggccaag ctcgacgagt cggaccgcta ctgcttcgtc
     1321 acgctcaacc gttggtccca cgaccccgtg ttcaccaagg gcgacgtctc ggtgctgctc
     1381 ctgtcggaga acctggcaga cctcaacccg cgc
//
LOCUS       DAOPHV010000705         1409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_135400_length_1409_cov_4.115953, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000705 DAOPHV010000000
VERSION     DAOPHV010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135400_length_1409_cov_4.115953"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLQ29_13555"
     CDS             complement(<1..185)
                     /locus_tag="PLQ29_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87720.1"
                     /translation="MGDYYPVDAGEVRKNGMRGVISTGAGIGLMLFNSLLNIPIVGWI
                     LGGGLVVEAKGLKKMIVA"
     gene            complement(312..1088)
                     /gene="folE2"
                     /locus_tag="PLQ29_13560"
     CDS             complement(312..1088)
                     /gene="folE2"
                     /locus_tag="PLQ29_13560"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HPG87721.1"
                     /translation="MVDVQSMRDERCIPIRKVGVKGVRYPITVLDRANSIQHTTATVN
                     LFVDLPHHYKGTHMSRFIEVFNEHRRDLSMPRFLAMLDELRDALDAETAYCDMHFPYF
                     IEKKAPVSGQPGMMSYDCSYEGSSSAEEREFWVSVQVPVQTVCPCSKAISEYGAHNQR
                     GLVKVRVKLGPFFWIEDLVAIVERSASSDVYTLLKREDEKYVTERSFERPMFVEDIVR
                     EVHGGLTALGIFPKFCVEAENFESIHNHSAYACAEYPLDR"
     gene            complement(1115..>1409)
                     /locus_tag="PLQ29_13565"
     CDS             complement(1115..>1409)
                     /locus_tag="PLQ29_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HPG87722.1"
                     /translation="LAEHTRRADILIACAGTPELIKADMVKPGACVIDVGVNRVDDQS
                     RAKGWRLVGDVEFDAVKDVAGSITPVPGGVGPMTITMLLFNTVESAERAAGRA"
BASE COUNT          263 a          451 c          424 g          271 t
ORIGIN      
        1 gcgacgatca tcttcttgag ccctttcgct tcgacgacga gtccgccgcc aaggatccat
       61 ccgacgattg gtatgttgag caatgaattg aacagcatca agccgatccc agcgcccgtc
      121 gagatgacgc cgcgcatacc gttcttgcgg acctcgcccg cgtcgaccgg atagtaatcg
      181 cccatagagt cctccttaat gatcggccgt cacctcgtcg ctcccatacg gcgagcggct
      241 tcgcgttgcc gcgacggcgg cgccaataag caatataccc aaaagctgac ggaccgtaaa
      301 ggaggcgaaa gctaccggtc gagcggatac tcggcgcacg cgtacgccga gtgattgtga
      361 atgctctcga aattctcggc ctcgacgcag aatttcggga aaatgccgag ggccgtaagg
      421 ccgccgtgca cctcgcgtac gatatcctcc acgaacatcg gccgttcgaa cgaccgctcg
      481 gtcacgtact tctcgtcctc tcgcttgagg agcgtgtaca cgtcgctgga cgcggaccgc
      541 tcgactatgg ccaccaggtc ctctatccag aaaaaaggcc cgagcttgac gcggacctta
      601 acgaggcctc gctggttatg ggctccgtac tcgctgatcg ccttggaaca ggggcagacg
      661 gtctgtaccg gcacctgaac ggagacccag aattcccgct cctcggccga cgacgagcct
      721 tcgtaggaac agtcgtagct catcatgccg ggctgcccgg ataccggcgc cttcttctcg
      781 atgaaatacg ggaaatgcat gtcgcagtac gcggtctccg cgtcgagggc gtcgcgtagc
      841 tcgtcgagca tggccaggaa gcggggcatg gacaggtcgc gcctgtgctc gttgaacacc
      901 tctatgaagc ggctcatgtg ggtgcccttg tagtggtgcg gcaggtcgac gaacaggttg
      961 accgtggcag tggtgtgctg gatagagttg gctcggtcca gcaccgtgat cggatatcgg
     1021 actcccttga cgccgacttt gcgtatcggg atgcatcgct cgtcgcgcat gctctggacg
     1081 tcgaccatgg ggcgcgccac cttcgccgcg gccctcatgc tcgcccggcc gcgcgctcgg
     1141 cggactcgac cgtattgaag aggagcatcg tgatggtcat cggaccgacg cctcccggca
     1201 ctggcgtaat cgagccggcc acatccttga cggcgtcgaa ctcaacatcg ccgacgagcc
     1261 gccagccctt ggccctgctc tggtcgtcga cccgattgac tccgacgtcg atgacacagg
     1321 cccccggctt gaccatgtcg gccttgatga gctcgggtgt accggcgcag gctatcagga
     1381 tgtccgcacg cctcgtatgc tccgccagg
//
LOCUS       DAOPHV010000706         1405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_136020_length_1405_cov_4.416296, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000706 DAOPHV010000000
VERSION     DAOPHV010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136020_length_1405_cov_4.416296"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLQ29_13570"
     CDS             <1..639
                     /locus_tag="PLQ29_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPG87723.1"
                     /translation="PYSVVLFDEIEKAHPDVFNLLLQLLEEGELRDNLGHAVSFRNCV
                     VIMTSNAGTRELTSGVRLGFGAETRRFDYDAVKSAALAELKRLFNPEFINRVDQVVVF
                     HPLDRDQVRVVLELELSRLRRRLAERGVSLELDRKAMDRLIRDGYDEAYGARPMRRMI
                     SKLIEDPLAAIMVEGRCPPGATARVREKDGELSIRVSTPQRAPSVPSAVSKD"
     gene            complement(655..>1405)
                     /locus_tag="PLQ29_13575"
     CDS             complement(655..>1405)
                     /locus_tag="PLQ29_13575"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HPG87724.1"
                     /translation="TEYADADRYRELGDILMANSGQALSGPSLRAEDFFRGGEVVIRV
                     DPSKSVVDNAKALYEKAKKARSGALETAAELDELREAIAALDEERRRVEATENPYAIR
                     AFLAKRRTAPSQGPKRFPGLSLERDGWLFLIGRSAVENDELLRRHVRGNDLWFHARDY
                     SGSYVFVKAKANKSVPLEIMLDAGSLALYYSKGRSSGAGDLYYTQAKYLRRAKNGPKG
                     LVIPTQEKNLRVKLDEGRLKELRRLIGRDGD"
BASE COUNT          218 a          450 c          486 g          251 t
ORIGIN      
        1 ccgtatagcg tggtgctgtt cgacgagatc gagaaggccc atccggacgt gttcaacctg
       61 ctcctgcagt tgctcgagga gggcgagctg cgcgataacc tgggacacgc cgtttcgttc
      121 aggaactgcg tcgtgatcat gaccagcaac gccggcaccc gcgagctgac gagcggggtt
      181 aggctgggct tcggggccga gacgaggcgt ttcgactacg atgccgtcaa gtcggcggcg
      241 ctggccgagc ttaagcgtct attcaacccg gaattcatca accgcgtcga ccaggtggtc
      301 gtcttccacc cgctcgaccg cgaccaggtg cgcgtcgtgc tcgagctgga gctgtccagg
      361 ctacgtaggc gcctcgccga gcgtggcgtc tcgctggagc tcgaccgcaa ggcgatggat
      421 cggctgatcc gcgacggcta cgacgaggcc tacggagcca ggccgatgcg gcgcatgatc
      481 tccaagctca tcgaggatcc gctcgcggcc atcatggtcg agggccgctg ccctcccggg
      541 gcgacagcga gggtgcgcga gaaggatggc gagctgtcga tcagggtgtc gacgccgcaa
      601 cgcgcgccgt ccgtaccgag cgccgtctcc aaggactgag cctcgccacc gaggttagtc
      661 gccgtcccgg ccgatgaggc gcctgagctc cttgagccgt ccctcgtcga gcttgacgcg
      721 caggttcttc tcctgggtcg ggataaccag gcccttgggg ccgttcttgg cccgccggag
      781 gtacttggcc tgcgtgtagt ataggtcgcc ggcgccggat gagcggccct tcgagtagta
      841 cagggccagg gagccggcgt cgagcatgat ctcgagcggc acgctcttgt tcgccttggc
      901 cttgacgaac acgtacgatc ccgagtagtc gcgcgcatgg aaccacaggt cgttgccgcg
      961 gacatggcgg cgcaggagtt cgtcgttctc gaccgccgag cggccgatca ggaacagcca
     1021 gccgtcgcgc tcgagcgaca gtcccgggaa gcgcttcggc ccctgggacg gggcggtcct
     1081 ccgcttggcc aggaaggcgc ggatagcgta agggttctcg gtggcctcga cgcgccgtcg
     1141 ctcttcatcg agcgccgcga tggcttcgcg tagctcgtcg agctcggcgg ccgtctccag
     1201 ggcgccagag cgggccttct tggccttctc gtagagggcc ttggcgttgt cgacaacgct
     1261 cttggatgga tcgacgcgta tgaccacctc gccaccgcgg aagaagtctt ccgctctgag
     1321 cgacggaccc gatagcgcct ggcccgagtt ggccatcagg atatctccca gctcgcggta
     1381 gcggtcggcg tcggcgtact cggtg
//
LOCUS       DAOPHV010000707         1405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_136033_length_1405_cov_3.662222, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000707 DAOPHV010000000
VERSION     DAOPHV010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136033_length_1405_cov_3.662222"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLQ29_13580"
     CDS             <1..580
                     /locus_tag="PLQ29_13580"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPG87725.1"
                     /translation="QDVANGAIRPGSENPFRDAPCEPDEAGVLALYRYKTGRYTFSLP
                     LALGAAIAGADEQTSQALERAGESLGVLFQLKDDELGLFADEDELGKPIGADVREDKK
                     TLLRLYLFALASGDERARLESMFGNPSAGAAEIEYLRSMAIGLGARERLEAAMRRYAA
                     VAETQAAIVRATTSSARAAFDELVRYSLERRS"
     gene            complement(624..>1405)
                     /locus_tag="PLQ29_13585"
     CDS             complement(624..>1405)
                     /locus_tag="PLQ29_13585"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPG87726.1"
                     /translation="VSLFVYRDQDPYLQAASVLLVINAQYLIPNRFSFSVVASLIVAA
                     AGAVSLVYGHGALSPSVMNAFVVDFVLMALLSALIWLRTCRSRRREYSKSLELEYISK
                     TDQLTGLGNRRYLAERYAEAKARMMRYGEGSAIIMADLDYFKALNDNYGHEEGDKVLK
                     EASRRFSESLRSEDSLCRWGGEEFVALISYATLDAALESAKRLQSALRSTPMEVVGTV
                     RASFGVTMVGTDETLDDTVARADRALYRAKNAGRDRVELEP"
BASE COUNT          237 a          491 c          458 g          219 t
ORIGIN      
        1 gcaggacgtg gccaacggcg ccatcaggcc cggctcggag aacccgttcc gcgacgcgcc
       61 ctgcgagccg gacgaggccg gcgtgctcgc gctataccgc tacaagaccg gccgctatac
      121 cttcagcctg ccgctggccc tcggcgcggc cattgccgga gccgacgagc agacgagcca
      181 ggcgctcgag cgcgccggcg agagcctcgg cgtcctattc cagctcaagg acgacgaact
      241 gggcctgttc gccgatgagg acgaactcgg aaagcccatc ggcgccgacg ttcgggagga
      301 caagaagacg ctcctgcgcc tgtacctgtt cgcgctcgct tccggcgacg aacgcgcccg
      361 gctcgagtcc atgttcggga acccgtccgc cggcgcggcc gaaatcgagt acctgcgctc
      421 gatggcgatc gggctgggcg cgcgcgagcg gctcgaggcc gcgatgcgcc gctacgccgc
      481 cgtggccgag acccaggccg ccatcgttcg cgccacgacg tcttcggcca gggccgcgtt
      541 cgacgagctg gtcaggtaca gcctggaacg tcgttcttag ccgaacggcg ttctaagccg
      601 aacgtcgttc ttagcccggg cagctacggc tccagctcca cccggtcccg gccggcgttc
      661 ttggcccggt acagcgcccg gtcggccctg gccaccgtgt cgtcgagcgt ctcgtcggtg
      721 ccgaccatcg tcacgccgaa gctggcgcga acggtaccga ccacctccat cggcgtcgac
      781 ctgagggccg actgcaggcg cttggccgac tcgagcgccg cgtcgagcgt ggcgtacgat
      841 atgagcgcca cgaactcctc gcctccccat cggcacagcg aatcctcgga ccggagcgat
      901 tccgagaagc gcctggaggc ttccttgagc accttgtcgc cttcctcgtg cccgtagttg
      961 tcgttgagcg ccttgaaata gtcgaggtcg gccatgatta tcgcgctgcc ctctccgtac
     1021 cgcatcatcc tggccttagc ctcggcatag cgctcggcca ggtagcgacg gttgcccagt
     1081 ccggtcagct ggtcggtctt ggagatgtac tccagctcga gcgacttgga gtattcccga
     1141 cgcctggacc tgcaggtccg tagccagatc agggccgaca ggagggccat cagcacgaag
     1201 tcgacgacaa aggcgttcat caccgaaggc gacagcgcgc catgcccgta cacaaggctg
     1261 acggcgcccg ccgcggccac gatcagcgac gcgaccaccg aaaacgagaa tcggttcggg
     1321 atcaggtact gggcgttgat caccagcagc acgctggccg cctgcaggta cgggtcctgg
     1381 tcgcggtaga cgaacaggga gacgc
//
LOCUS       DAOPHV010000708         1401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_136631_length_1401_cov_3.294205, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000708 DAOPHV010000000
VERSION     DAOPHV010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136631_length_1401_cov_3.294205"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLQ29_13590"
     CDS             complement(<1..423)
                     /locus_tag="PLQ29_13590"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPG87727.1"
                     /translation="MRVVEAAAITEAVRKLCIESNYVLPADVRGALEAGAQAEESPVG
                     RAILADILANADLAAADRMAICQDSGFAVCFVELGQEAAVVGGDFEAAIHEGVRRGYA
                     QGYLRASIVDDPVFERRNTGDNTPAVIHVRVVPGNRIRI"
     gene            complement(584..>1401)
                     /locus_tag="PLQ29_13595"
     CDS             complement(584..>1401)
                     /locus_tag="PLQ29_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87728.1"
                     /translation="NIVFAGTIIETASDDSQVETGKVELIYKKETSRFSFHSELLIAD
                     PENVLGNGSDFYAYTVVDIPETTINDDYSFLASASTLAMLMPGTINEFQYLDAIEIYS
                     GIGNTGDPVVGFASGAFTSSLVSDHAALDGADFASMVSAEGIPLPTAVFQPIKDAIIA
                     ARSVLAAAIEDNGAHMLGYRIIESPVTSTYVYNAPEYATDGVYLVDADGNRLKNSSGE
                     DIEFARDAPDATMESKVVSNVVKLVSGFPTQAWREASRLPGVYATRSTYVDDR"
BASE COUNT          280 a          424 c          400 g          297 t
ORIGIN      
        1 tatcctaatc cggttgcctg gcacgacgcg tacgtggatg acggcggggg tattgtctcc
       61 ggtgttccgg cgctcgaaga cgggatcgtc gacgatggac gcgcgcaggt agccctgggc
      121 gtagcctcgg cgcacgccct cgtgtatcgc ggcctcgaag tcgccgccga ccactgccgc
      181 ctcctgcccg agctccacga agcatacggc gaagcctgag tcctggcaga tggccatgcg
      241 gtcggcggcc gctaggtcgg cgttcgcaag gatgtcagcg aggatggctc ggccgacggg
      301 cgactcctcg gcctgggctc cagcctcgag cgccccgcgc acgtcggccg ggaggacgta
      361 attcgactcg atgcacagtt tcctgaccgc ctcggtgata gcggccgcct cgactacacg
      421 catgatgccc ccgttacgcg cagtttcttc cgagttacct acctaagctt ccgaggggag
      481 tatacgcgcc gccgagcggc tcgggaagag ccgactcggc gacgccaaag cggaaattgc
      541 agttctgcta atgatcttag gtcagcgtac ctggttacta ccgctatcta tcgtcgacat
      601 aggtagacct agttgcatag acgccaggta gccttgaagc ttccctccaa gcctgggtcg
      661 gaaaaccgct taccagcttg accacgtttg atactacttt cgactccatg gtggcatccg
      721 gcgcatcgcg agcgaattcg atatcctcgc cgctcgagtt cttcaggcga ttcccatccg
      781 catcgacgag atacactcca tccgtagcat attccggtgc gttataaaca taggtactcg
      841 taaccgggct ctcgatgatc ctatagccga gcatgtgagc gccgttatcc tcgattgcgg
      901 cggcgagcac gcttcgggcg gcgatgatcg cgtccttgat gggttggaac acggcggttg
      961 gcagcggtat accctcggcg ctcaccatcg acgcaaaatc cgcgccgtcg agcgccgcgt
     1021 ggtccgagac cagagacgac gtaaacgcgc ctgaagcgaa gccaaccact gggtcacccg
     1081 tattccctat cccgctatag atctctatcg cgtcgagata ctggaattca ttgatcgtcc
     1141 cgggcatgag catggccaga gtgctagccg atgccaagaa tgagtaatca tcgttgatcg
     1201 tagtttctgg aatatccact accgtatacg cgtagaaatc cgaaccatta ccgagcacat
     1261 tttcgggatc tgcgatcagt agctcggagt ggaaagaaaa cctcgaagtc tcctttttgt
     1321 agatgagctc gactttgccg gtctcgacct ggctatcgtc ggaggcggtc tcaattatcg
     1381 ttcccgcgaa aactatattc c
//
LOCUS       DAOPHV010000709         1399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_136904_length_1399_cov_2.882440, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000709 DAOPHV010000000
VERSION     DAOPHV010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136904_length_1399_cov_2.882440"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLQ29_13600"
     CDS             complement(<1..258)
                     /locus_tag="PLQ29_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87729.1"
                     /translation="MARVIVDGIGLEYSILAAASGAADGERSEPPTVALLNGIGMSIA
                     HWRPVAERLAAAGYRVLLHDFRGQLLSERPTGPYSFEAHAHD"
     gene            complement(261..965)
                     /locus_tag="PLQ29_13605"
     CDS             complement(261..965)
                     /locus_tag="PLQ29_13605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG87730.1"
                     /translation="MILDIKKLETGYGKLRVLRGVDLSVGRESVGLFGPNGAGKTTLI
                     NAIMGMVRPWAGSIEFEGAPIGGRPTHLVARAGIALVPQERELFPGMSVADNLELGAA
                     YVPRGRDIIEKQLDTVLSLFPILKERYRQLVGTMSGGQQRMVAIGRALMANPILLILD
                     EPSLGLQPSIVAEVFDRLSVLKKDVAILVTEQNVRESLRAIDRGYVIENGRVLLEDTA
                     EGLAKNPHVVSAYLGL"
     gene            complement(962..>1399)
                     /locus_tag="PLQ29_13610"
     CDS             complement(962..>1399)
                     /locus_tag="PLQ29_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPG87731.1"
                     /translation="VLENVMVGAVFSRGKSLREARLSAEGICDELGLGGLDRDTAQLS
                     ILEIKKMEIARALANEPEVLFLDEVMAGLNSDETKQMIETVRRIAGERRMAVGVVEHV
                     MGVIRELTQRVIVLEAGELIAEGPYEKVSKDPRVIEAYLGGAA"
BASE COUNT          210 a          501 c          443 g          245 t
ORIGIN      
        1 gtcgtgcgcg tgggcctcga acgagtacgg cccagtcggc cgctcggaca ggagctgccc
       61 ccggaagtcg tggagcagga cgcggtaccc ggcggcagct agccgctcgg ctaccggccg
      121 ccagtgggcg atcgacatgc cgatgccgtt gagcagcgcg accgtcggcg gctcgctccg
      181 ctccccgtcg gcggccccgc tcgccgcggc caggatcgag tactcgaggc cgatcccgtc
      241 gacgatcacg cgcgccacgg ctacagcccc aggtaggcgg acacgacgtg ggggttcttg
      301 gccaggccct cggccgtgtc ctcgagcagc acccgcccgt tctcgatcac gtagccgcgg
      361 tctatggcac gcaggctctc gcgtacgttc tgctcggtga cgagtatggc cacgtccttc
      421 ttgaggacag agagccggtc gaacacctcc gccacgatcg acggctggag tccgagcgac
      481 ggctcgtcga gaatcaagag gatcgggttg gccatcagcg cgcggcctat cgccaccatg
      541 cgttgctggc caccggacat cgtgccgacg agctgcctgt agcgctcctt gagtatcggg
      601 aagaggctca gcaccgtgtc gagctgtttc tcgatgatat ccctgccgcg cggcacgtag
      661 gccgccccga gctccaggtt atcagcgacg ctcatgccgg gaaacagctc gcgctcctgc
      721 ggcacgaggg ctatgccggc cctggccacg aggtgcgtcg ggcgtccgcc tatgggcgcc
      781 ccttcgaatt cgatagatcc ggcccacggc ctgaccatgc ccatgatcgc attgatcagc
      841 gtggtcttgc ccgccccgtt cgggccgaac aggcccaccg actcgcggcc cacggacagg
      901 tccacgccgc ggagcacccg cagcttgccg tagccagtct cgagcttctt gatgtccagt
      961 atcatgcggc tcctccgagg taggcctcga tcacgcgggg gtccttggag accttctcgt
     1021 acggtccctc ggctatcagc tcgccggcct cgagcacgat cacgcgctgt gtcagctcgc
     1081 ggatgacgcc catgacgtgc tccacgaccc cgaccgccat tctgcgctcg ccggcgatgc
     1141 gcctgacggt ctctatcatc tgcttggtct cgtcgctgtt gagcccggcc atcacctcgt
     1201 cgaggaacag cacctcgggc tcgttcgcca gggcgcgggc tatctccatc ttcttgatct
     1261 cgaggatgga cagctgggcg gtatcgcggt ccagcccgcc caggcctagc tcgtcgcaga
     1321 tgccctcggc cgagagcctg gcctccctga ggctcttgcc tcgactgaag acggcgccga
     1381 ccatcacgtt ctcgagcac
//
LOCUS       DAOPHV010000710         1392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_137938_length_1392_cov_2.686612, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000710 DAOPHV010000000
VERSION     DAOPHV010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137938_length_1392_cov_2.686612"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..598
                     /gene="pth"
                     /locus_tag="PLQ29_13615"
     CDS             23..598
                     /gene="pth"
                     /locus_tag="PLQ29_13615"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPG87732.1"
                     /translation="MIKLYAFLGNHGREYSGNRHNVAWLFLESLPFYASLQWKRGYKG
                     RWAQRETPLGRAWFIVPETYMNLSGDSVAEIARFYKVEPGEVLVVHDELELGFGFFGF
                     KKGGGLGGHNGLRSMKERLGTADFMRLRFGIGRPSHDDVAGYVLSDFSRDEREKLECS
                     VFPRAATALELCADRGFDEALAAYAKVNALA"
     gene            complement(611..>1392)
                     /gene="era"
                     /locus_tag="PLQ29_13620"
     CDS             complement(611..>1392)
                     /gene="era"
                     /locus_tag="PLQ29_13620"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPG87733.1"
                     /translation="SPVPQTTRNTVRGIKTGEYYQLVFLDTPGLHDSEKAMNRRLRDL
                     ALRALQDADLVLYVIDTTRAPGPEEEAIIEKVGPFIGKTLVAVNKIDARDSDAPRARA
                     FLDERMPGSRAFELSAEQGTGVDALVAALAEAAPVGPEYYPPEYYTDQEPVFRIAEVI
                     REKIFLHTRNEVPHAVFVEYTAHHTLDDGTLVYEGDLVVERDTQKGILIGAGGSMIRL
                     IREEAERDLEDIFGYPVRLSIKVRVQPDWKKDDKILKKLIQ"
BASE COUNT          205 a          457 c          484 g          246 t
ORIGIN      
        1 gaatcgctat gctgtgggcc gtatgatcaa gctgtacgcc ttcctgggca accacggccg
       61 ggagtattcc ggcaacaggc acaacgtggc gtggctcttc ctcgagtcgc tgccgttcta
      121 cgcgtcgctc caatggaagc gaggctacaa gggccgctgg gcccagcgcg agacgccgct
      181 cgggcgcgcc tggttcatcg taccggagac gtacatgaac ctgtccggcg acagcgtcgc
      241 cgagatagcc aggttctaca aggtggagcc cggagaggtc ctcgtggtcc acgacgagct
      301 ggagctcggc ttcggcttct tcggcttcaa gaagggcggc ggcctcggcg gccacaacgg
      361 cctcaggtcg atgaaggagc ggctcggcac ggccgacttc atgcggctgc gcttcggcat
      421 aggccggccg agccacgacg acgtggccgg ctacgtgctg tccgacttct cgcgcgacga
      481 gcgggagaag ctcgagtgct cggtgttccc gcgcgccgcg accgccctcg agctatgcgc
      541 cgatcgcggc ttcgacgagg cgctcgcggc ctacgccaag gtcaacgcgc tcgcgtgagg
      601 ccggccggcc ctactggatc agcttcttga ggatcttgtc gtccttcttc cagtcgggct
      661 ggacgcgcac cttgatcgac agcctgaccg ggtagccgaa gatgtcctcg aggtcgcgct
      721 cggcctcctc gcgtatcagc cttatcatcg acccgccggc ccctatcagg atgcccttct
      781 gggtgtcgcg ctcgacgacc aggtcgccct cgtagacgag ggtgccgtcg tcgagcgtat
      841 ggtgcgccgt atactcgacg aacacggcgt gcggcacctc gttcctcgta tgcaggaaga
      901 tcttctcgcg tatcacctcg gcgatgcgga acacgggctc ctggtcggtg tagtactcgg
      961 gcgggtagta ttccggcccg acaggggcgg cctcggccag cgccgcgacg agcgcgtcga
     1021 cgccggtacc ctgttccgcg gacagctcga aggcgcgcga gccgggcatg cgctcgtcca
     1081 ggaaggcgcg cgcgcgcggc gcgtcggagt ccctcgcgtc gatcttgttg accgcgacga
     1141 gcgtcttgcc gatgaacggc ccgaccttct cgatgatagc ctcctcctcg gggcccggcg
     1201 cgcgggtggt gtcgatcacg tacagcacga ggtcggcgtc ctggagcgcc cgcagggcca
     1261 ggtcgcgcag ccggcggttc atggccttct cggagtcgtg caggccgggc gtgtcgagga
     1321 acacgagctg gtagtactcg ccggtcttga tgccgcggac ggtgttgcgc gtggtctgcg
     1381 gcacgggcga ga
//
LOCUS       DAOPHV010000711         1391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_138028_length_1391_cov_4.260479, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000711 DAOPHV010000000
VERSION     DAOPHV010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138028_length_1391_cov_4.260479"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1391
                     /locus_tag="PLQ29_13625"
     CDS             <1..>1391
                     /locus_tag="PLQ29_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87734.1"
                     /translation="QFGDGDRYFGVCAMMATMPGLPMFGHGQFEGFSEKYGMEYRRAY
                     RDERPNEALVRRHERDIVPLLKRRRLFSGVEHFLLFDLVRDDGSVDENVFAYSNGHGP
                     ERALVLFNNEWERTAGTIRRSCPFADKRPDGTRPGTTRSLAEGLGLSGRPGRFVMMTE
                     AGTWKRYLRRSDELARDGLRVALEGFQCQVFVDVTEVDDDEAGSYARLCDALGGRGVP
                     DLGWALQDLEFAPAYDAWKAALGVGYFGREPRPEPDQEATAAFIEALAAAALPIGTAA
                     PTGESASPDYRAMAMAAIEGRERLERASSASTAPLAARVAAVPGAREAVAALAALLPI
                     AELAGGLAEAPKARALAESAGLGRKLREAMAEAGLDPGLAVAASGFAMAMLGRLGRVS
                     ERLGGGPGGISGRATARVILADDEARALFRVNRWNNESWIDADRYRLGSTLYAVAAVA
                     FGGLGAAEAVRPL"
BASE COUNT          192 a          501 c          504 g          194 t
ORIGIN      
        1 tcagttcggc gacggagacc gctacttcgg cgtctgcgcg atgatggcca cgatgcccgg
       61 cctgccgatg ttcggccacg gccagttcga gggcttctcg gagaagtacg gcatggagta
      121 ccgccgggcc taccgcgacg agagaccgaa cgaggccctg gtccgtcgcc atgagcgcga
      181 tatcgtgccg ctcctcaagc gccgccgcct cttctccggc gtcgagcatt tcctgctctt
      241 cgacctggtc cgtgacgacg gctcggtcga cgagaacgtg ttcgcctact cgaacggcca
      301 cgggccggag cgggccctcg tgctgttcaa caacgagtgg gagcggaccg cggggacgat
      361 acgccgctcc tgccccttcg ccgacaaacg tcccgacggg accaggcccg gaacgacgag
      421 gagcctggcc gaggggctcg gcctgtccgg ccgacccggc cgcttcgtga tgatgaccga
      481 ggccggcacc tggaagcgct acctcaggcg ctcggacgag ctggcccgcg acggcctgcg
      541 ggtcgccctc gagggcttcc agtgccaggt tttcgtcgac gtaaccgagg tggacgacga
      601 cgaggccggc tcctacgcga ggctctgcga cgcgctcggc ggacgcggcg ttcccgacct
      661 cggctgggcg ctccaggatc tcgagttcgc gccggcctac gatgcctgga aggccgccct
      721 cggcgtcggc tacttcggac gggagcctcg gcccgagccg gaccaagagg ctacggccgc
      781 cttcatcgag gcgctggccg ccgccgcgct accgatcggg actgcggccc cgaccggcga
      841 gtccgcgtcg cccgactacc gggccatggc gatggcggcc atcgaaggcc gcgagcgcct
      901 cgagcgcgca tcgtccgcct ccacggcgcc gctcgccgcc agggtcgccg ccgtaccggg
      961 cgcccgcgag gccgtggccg cgctcgcagc cctgctaccg atagcggaac tggcgggcgg
     1021 actcgccgag gccccgaagg cgcgggcgct agccgagtcg gccggcttgg gacgcaagct
     1081 acgcgaggcg atggccgagg ccgggctcga tccaggcctg gccgtcgctg cctccggctt
     1141 cgccatggcg atgctcggac gcctgggcag ggtctccgaa cggctgggag gcggcccggg
     1201 cggcatctcg ggacgcgcta cggccagggt catcctggcc gacgacgaag cccgcgccct
     1261 attcagggtg aaccgctgga ataacgagag ctggatagac gccgaccggt accggctcgg
     1321 ctcgacgctg tacgccgtgg cggctgtggc gttcggcggg ctcggcgccg ccgaggcggt
     1381 ccgcccgctg t
//
LOCUS       DAOPHV010000712         1391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_138062_length_1391_cov_3.074102, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000712 DAOPHV010000000
VERSION     DAOPHV010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138062_length_1391_cov_3.074102"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..>1391
                     /locus_tag="PLQ29_13630"
     CDS             225..>1391
                     /locus_tag="PLQ29_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPG87735.1"
                     /translation="MRFSSRLGPRFEPNAFSSLQAELVSRGVELLNMSDSNPTRHGLS
                     PCGVIGALADARCLAYRPDPRGLESARSALAGRFGGEAASYFLSASTSEAYAWIFKLL
                     CDPGDAVLVPKPGYPLFDSLAGLESVRAVPYRLEYRHPEGWSVDLEDLRLVAKASGAK
                     AVVLINPNNPSGSYIRADERRRIVGLCSELGMAVIADEVFFPYALEAVGGMARLAGEE
                     ACLTFALDGLSKLLCLPQLKLGWIRISGPRDQVDEAAARLELIADTYLSAGAPVMNAL
                     PSLLPGVDDFVGLVLGRLRSNLSEARTVFGGDGSPYRVLRCDGGWTALLEFPRYQGEE
                     SVILGLLEHASVAIQPGYFFDMEREGSLALSLILEPEVFAEGVRRIKAYIDSMGA"
BASE COUNT          236 a          449 c          438 g          268 t
ORIGIN      
        1 atctacctga agcagccgcc tttgcgcccg agatacgagt acgagtcgac tatcgacgca
       61 tatttacggc tcaaaggcat cgaccggctg atagaagaat acgagcgccg ccacggcggg
      121 gccaagcggc acgcggccgg accgtcgagc gtgaccacga cgctgcaatg acgtcatcgt
      181 aagccgggcc ttgccgtcaa ggggcgttcc ggctaggatg cggcatgcgt ttttcatcac
      241 gcctcggccc acgcttcgag ccgaacgcgt tctcttccct ccaggccgag cttgtcagcc
      301 gtggggtcga gctgctcaat atgtccgact cgaatccgac gcgccacggc ttgtcgccat
      361 gcggtgtcat cggcgcgctg gccgatgcta gatgcctcgc atatcggccc gaccctcgcg
      421 gtctcgagtc cgcccgatct gccctcgccg ggcgcttcgg cggcgaggcc gcgtcgtact
      481 tcctgtcggc cagcacgtct gaagcctatg cctggatctt caagctattg tgcgatcccg
      541 gcgacgcggt actcgtgcct aagccaggct atcccctgtt cgattctctc gccggcctcg
      601 agtccgtccg cgccgtaccg tatcgtctcg agtatcgcca cccggaagga tggtccgtcg
      661 acctcgagga cctacggctg gtcgcgaagg cgtcgggagc caaggctgtc gttctcatca
      721 acccgaataa cccgagcggc tcgtatatca gggccgacga gaggcgccgg atagtcgggc
      781 tatgctccga gcttggcatg gcggtcatcg ccgatgaggt gttcttcccg tatgcgctcg
      841 aagccgtagg cggcatggcc cggctagccg gcgaggaggc ttgccttacc ttcgccctcg
      901 acggattgtc gaagctactt tgcctgcccc agctgaagct gggctggata cggatttccg
      961 gcccgcggga ccaggtcgac gaggcggctg cgaggctcga gctgatcgcc gatacctacc
     1021 tgtccgccgg ggcgcccgtg atgaacgcgc taccgagcct gctacccgga gtcgatgatt
     1081 tcgtaggttt ggtcctcggg cgtctgcgct caaacctgag cgaggcgcga acggtattcg
     1141 gaggcgacgg atctccgtac cgggtactgc gctgcgacgg cggatggacg gccctcctcg
     1201 aattcccacg ctaccaaggc gaggagagcg tgatccttgg cctcctcgag catgcgtcgg
     1261 tagcaatcca gccgggatac ttcttcgata tggaacgaga gggctctcta gccctaagtc
     1321 tgatcctcga acccgaggtc ttcgccgaag gggtccgcag gatcaaggcc tacatagact
     1381 cgatgggagc g
//
LOCUS       DAOPHV010000713         1390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_138182_length_1390_cov_4.268914, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000713 DAOPHV010000000
VERSION     DAOPHV010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138182_length_1390_cov_4.268914"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..435)
                     /locus_tag="PLQ29_13635"
     CDS             complement(19..435)
                     /locus_tag="PLQ29_13635"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPG87736.1"
                     /translation="MDSERNDEDGGYSGPILYYTDAVIDHFTNPRNVGEMADDEADGY
                     ACIGDPGCGDEMKLWIAVRDDRIERIAFKSYGCPGAISTSSMLTVLAEGRTLDEALAI
                     TDDDVVGALGGIPENKQHCSLLGVKALHAAIASSRR"
     gene            567..1211
                     /locus_tag="PLQ29_13640"
     CDS             567..1211
                     /locus_tag="PLQ29_13640"
                     /inference="COORDINATES: protein motif:HMM:NF017985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HPG87737.1"
                     /translation="MNYRRAVASMLFALCAAPALWAHPHVFIDSSLEFEMSGRDCVGI
                     VVSWTFDVVFSADIIGQFDKDRDGAFNEAETATVQAKAFSNLRKYGYFTFVRKGSVRT
                     CPEAVEAFGVSQSDGRVTYRFRVPLEGKGVSDDFSVATFDSTFYCATKYVDGAASVRW
                     IGDRPADEPTIAIAANKDYPVYYNPLGSATDSRVYTKWEKGLQTAYPEEIRVRF"
     gene            complement(1201..>1390)
                     /locus_tag="PLQ29_13645"
     CDS             complement(1201..>1390)
                     /locus_tag="PLQ29_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87738.1"
                     /translation="RCVSTIGPCEPGARTVGNGGASSSLDVNGLARSGAAASAMAQAS
                     VAALTAGRPILIIVAIRT"
BASE COUNT          241 a          470 c          446 g          233 t
ORIGIN      
        1 gtagccgtcg cggcgcggct accgcctgga cgaggctatg gccgcgtgca gcgccttgac
       61 gccgaggagc gagcagtgct gcttgttctc cgggatgccg ccgagcgcgc cgaccacgtc
      121 gtcgtcggta atcgcgagcg cctcgtcgag ggtccggccc tcggcgagga cggtgagcat
      181 gctgctcgtc gagatggcgc cggggcagcc gtacgacttg aacgcgatac gctcgatcct
      241 atcgtcgcgc acggctatcc atagcttcat ctcgtcgccg cagccggggt cgccgatgca
      301 ggcgtagccg tccgcctcgt cgtcggccat ctcgccgacg ttacgcggat tggtgaagtg
      361 gtcgatgacg gcgtcggtat aatacaggat cggccccgaa tagccgccat cctcgtcgtt
      421 acgctcggaa tccatggcac aagctcctat ttcgtgccaa tatagcatat gccaatattt
      481 ccggacaatg ggatccgaga ttttctacgc ccggaacgct tcacgatccg gccgcttgac
      541 tatagaatcg acggcgagga gcacccatga actaccgacg agccgtagcc tctatgctgt
      601 tcgcgctatg cgcggcgcca gccctctggg cccatccgca cgtcttcatc gactcgagcc
      661 tcgagttcga gatgagcggc cgggactgcg tcgggatagt ggtatcatgg accttcgacg
      721 tcgtcttcag cgccgacatc atagggcagt tcgacaagga ccgcgacggc gccttcaacg
      781 aggccgagac cgcaacggtg caggccaagg cgttctcgaa cctccgcaag tacggctact
      841 tcaccttcgt gcgcaagggt tccgtcagga cctgcccgga agcggtcgag gccttcgggg
      901 tcagccagag cgacggccgc gtgacctacc gcttcagggt cccgctcgag ggcaagggcg
      961 tctcggacga cttcagcgtc gccaccttcg actcgacctt ctactgcgcg accaagtacg
     1021 tcgacggcgc ggcgagcgtc cgctggatag gcgaccggcc ggccgacgag ccgaccatcg
     1081 cgatagccgc caacaaggac tacccggtct actataaccc gctcggcagc gccaccgact
     1141 cccgcgtcta cacgaagtgg gagaaggggc tccagacggc ctaccccgaa gagatacggg
     1201 tcaggttctg atggcgacga tgatgaggat gggccgcccc gccgtcaggg cggccacgct
     1261 ggcctgcgcc atggcgctcg ccgcggcccc gctcctcgcg agcccgttca cgtcgagcga
     1321 cgacgacgcg ccgccgttgc cgacggtgcg cgcgcccggc tcgcagggcc cgatggtcga
     1381 gacgcagcgc
//
LOCUS       DAOPHV010000714         1390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_138248_length_1390_cov_2.591760, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000714 DAOPHV010000000
VERSION     DAOPHV010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138248_length_1390_cov_2.591760"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLQ29_13650"
     CDS             <1..410
                     /locus_tag="PLQ29_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87739.1"
                     /translation="WALAMKAAAAVNESAQAIFRKVDEEADAVAGAMDEIGRGLSEIS
                     AGSGEILQGTSESVQITTTVRDASRLMGEAIHENERDLGRLSDRLEAVRLSLRSVVEG
                     FQKVRVESDSLGEAGRLSELALTNLMESLEASR"
     gene            complement(417..929)
                     /locus_tag="PLQ29_13655"
     CDS             complement(417..929)
                     /locus_tag="PLQ29_13655"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPG87740.1"
                     /translation="MKARSICHAGLTVSDFGAAVRWYHDTFGFLLVSEDVLEPERTKE
                     LFPLYGVEGARVRMGFMRAPGGSVLELFEFSPKAERREPTWNAPGFTHLAMNVSGVSS
                     WYERLKADGESFVTPPQRTGSVDWVFMRDPDGNLVELIDLKALRPAIRLVGGALGAVM
                     KRGKFAKHYG"
     gene            complement(939..>1390)
                     /locus_tag="PLQ29_13660"
     CDS             complement(939..>1390)
                     /locus_tag="PLQ29_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87741.1"
                     /translation="FKGLLAMSAALGATKPPATEAERELVVKLYFYLLSSGISQLNKA
                     GHPDVARWVAASPDRVYAWSVEGKPELSAYLRLKAGNSRAARGVYERSKPFFTMSFDS
                     TASALGILMSIDDMVEATKAGRIIMKGAPEYGAAIGEYMMLVGAYAK"
BASE COUNT          229 a          474 c          470 g          217 t
ORIGIN      
        1 cctgggcgct cgcgatgaag gccgccgccg ccgtcaacga gagcgcccag gccatcttca
       61 ggaaggtcga cgaggaggcc gacgccgtcg ccggcgcgat ggacgagatc ggccgcgggc
      121 tatccgagat atcggccggt tccggcgaga tactccaagg cacctccgag tccgtacaga
      181 tcaccaccac ggtccgcgat gcctcgaggc tcatgggcga ggccatccat gagaacgagc
      241 gcgacctggg ccggttgagc gacaggctcg aagccgtgcg cctgtcgctc cggtcggtgg
      301 tcgagggctt ccagaaggtc agggtcgagt ccgactcgct tggcgaagcg ggccgcctct
      361 ccgaactcgc cctgaccaac ctgatggaga gcctcgaggc tagccgatag gcggcgctag
      421 ccgtagtgct tggcgaactt ccctcgtttc atgaccgccc cgagcgcgcc tccgaccagc
      481 ctgatagccg gccggagggc cttgagatcg atcagctcga cgaggttgcc atccgggtcg
      541 cgcatgaaca cccagtcgac cgacccggta cgctggggcg gcgtcacgaa gctctcgccg
      601 tccgccttga gccgctcgta ccacgaggac acgcccgaca cgttcatcgc gaggtgcgtg
      661 aagcccggcg cgttccaggt gggttcccgc cgttcggcct tcgggctgaa ctcgaagagc
      721 tcgagcaccg agcctcccgg tgcgcgcatg aagcccatgc gcacgcgcgc tccctccacc
      781 ccgtagagcg ggaatagttc cttggtccgc tccggttcca ggacgtcctc cgagacgagc
      841 aggaacccga aggtgtcgtg gtaccagcgc acggcggccc cgaagtcgga gaccgtcagg
      901 ccggcgtggc agatcgaacg agccttcacg gtagcctcct acttggcgta ggcgccgacg
      961 agcatcatgt actcgccgat cgccgccccg tactccgggg cgcccttcat gatgatgcgg
     1021 ccggccttgg tcgcctcgac catgtcgtct atcgacatga gtatgccgag cgcgctcgcg
     1081 gtggagtcga acgacatcgt gaagaacggc ttcgagcgct cgtagacgcc gcgcgcggcc
     1141 cggctgttgc cggccttgag ccgcaggtag gccgacagct cgggcttgcc ctcgaccgac
     1201 cacgcgtaga cgcggtcggg gctggcggcg acccagcgcg ccacgtcagg gtggccggcc
     1261 ttgttgagct ggctgatgcc cgacgacagt aggtagaagt acagcttgac cacgagctct
     1321 cgctctgcct cggtagccgg cggcttggtc gcgccgagcg ccgcggacat ggcgaggagc
     1381 cccttgaagg
//
LOCUS       DAOPHV010000715         1389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_138346_length_1389_cov_3.664168, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000715 DAOPHV010000000
VERSION     DAOPHV010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138346_length_1389_cov_3.664168"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLQ29_13665"
     CDS             <1..189
                     /locus_tag="PLQ29_13665"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPG87742.1"
                     /translation="CPGAISTSSMLTVLAEGRTLDEALAITDDDVVGALGGIPENKQH
                     CSLLGVKALRAAVASARR"
     gene            complement(226..564)
                     /locus_tag="PLQ29_13670"
     CDS             complement(226..564)
                     /locus_tag="PLQ29_13670"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPG87743.1"
                     /translation="MRIAIPLTGGKLSAHFGHCQEFAIIDADPDAKTIGVRDDVTPPP
                     HEPGLYPAWLAERGATHIIAGGMGQKAQELFARNKIAVVTGAPADDPEAIVGLFLRGH
                     LVTGANGCDH"
     gene            complement(594..902)
                     /locus_tag="PLQ29_13675"
     CDS             complement(594..902)
                     /locus_tag="PLQ29_13675"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPG87744.1"
                     /translation="MRYAVPTDDGETVGKVFGRASSFAIFDQADSSLVVHRNDGINAE
                     HGAGTGAASFLADKGVNVVLAPEVGPKASAGLAAAGIEVVVAATGTKLRAAIDAAIAK
                     "
     gene            complement(899..>1389)
                     /locus_tag="PLQ29_13680"
     CDS             complement(899..>1389)
                     /locus_tag="PLQ29_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87745.1"
                     /translation="RIVHAELEPGEDTSGKLVAQVRGRAAELAGEDAVIVVDAPPGIG
                     CPVIASITGCDLVVVVVEASASGVRDAARLVELVGSMRRRAMAIVNKSGLNQAMDDAA
                     RTMLKEAGIPVAGEIPFAPALRSVEESNRTWASLDCEAATRIRSALGAVDGAIGALKE
                     KR"
BASE COUNT          183 a          480 c          490 g          236 t
ORIGIN      
        1 tgccccggcg ccatctcgac gagcagcatg ctcaccgtcc tcgccgaggg caggaccctc
       61 gacgaggcgc tcgcgatcac cgacgacgac gtggtcggcg cgctcggcgg catccccgag
      121 aacaagcagc actgctcgct gctcggggtc aaggcgctgc gcgcggccgt cgcctccgcg
      181 aggcgatagc gtcgctgggc gagtcccgtc cggcgacgcc ccgtcctagt ggtcgcagcc
      241 gttggcgccg gtaacgaggt ggccccgcag gaacagcccg acgatggcct cggggtcgtc
      301 cgccggcgcg ccggtgacga cggcgatctt gttccgggcg aacagttcct gggccttctg
      361 gcccatgccg ccggcgatga tatgggtggc gcctcgctcg gcgagccagg cggggtagag
      421 accgggctcg tggggcggcg gcgtgacgtc gtcgcggacg ccgatagtct tggcgtcggg
      481 gtcggcgtcg atgatcgcga attcctggca gtggccgaag tgagcggata gcttgccgcc
      541 ggtcaacggt atggcgattc tcataggttt tcctttctat tcggaggcta atcttacttg
      601 gctatggcgg cgtcgatggc ggcgcgcagc ttcgtgccgg tcgcggcgac gacgacctcg
      661 atgccggccg cggcgagccc cgcggaggcc ttggggccga cctcgggggc tagcacgacg
      721 ttcacgccct tgtccgcgag gaaagaggcc gctccggtgc ccgcgccgtg ctcggcgttt
      781 atgccgtcgt tcctgtggac gaccaggctc gagtcggcct ggtcgaagat cgcgaagctc
      841 gaggctcgcc cgaagacctt gcctacggtt tcgccgtcgt cggtgggtac cgcgtatctc
      901 atcgtttctc cttaagggcg cctatcgcgc cgtctacggc gccgagcgcc gatcgtattc
      961 gggtagccgc ctcgcagtcg agcgaggccc acgtcctgtt cgattcctcg accgagcgca
     1021 gcgcgggggc gaacgggatc tccccggcta cggggatgcc cgcttccttg agcatggtcc
     1081 gcgccgcgtc gtccatcgcc tggttcaggc ccgacttgtt gacgatggcc atcgcccgcc
     1141 gcctcatcga gcctaccagc tcgacgagcc tcgcggcgtc gcgtacgccg gaagcgctcg
     1201 cctcgacgac gacgacgacc aggtcgcagc ctgtgatcga cgcgatgacc gggcagccaa
     1261 tgccgggcgg cgcgtcgacg actatcaccg cgtcctcgcc ggccagctcg gcggcgcgcc
     1321 cgcggacctg cgcgacgagc ttgccgcttg tgtcctcgcc gggctcgagc tcggcgtgca
     1381 cgatgcgca
//
LOCUS       DAOPHV010000716         1389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_138391_length_1389_cov_2.663418, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000716 DAOPHV010000000
VERSION     DAOPHV010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138391_length_1389_cov_2.663418"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PLQ29_13685"
     CDS             <1..358
                     /locus_tag="PLQ29_13685"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HPG87746.1"
                     /translation="ELADVPLILREEGSGAREYFKRMMQSAGIPFTVRWSSYSHSAII
                     DAVEHDLGVGVISGMLARKYSGGGRLHICEIADVELDRSFKLVYKKNKFITNILMRFT
                     QICGNTKYFNMAPEQL"
     gene            475..>1389
                     /locus_tag="PLQ29_13690"
     CDS             475..>1389
                     /locus_tag="PLQ29_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87747.1"
                     /translation="MQSSYNLWDPGVWSFVFALAILFLGMIAANALIRLVKPLRNMLI
                     PSSVLGGFMLVAFLAIMKAVTGKSLIEPSTLEMITYHGLGLGCTALALKVEKKTKRRH
                     AQRDIFNSALVTTSGYLLQVVIGLSVSFALYFVLAEVWPASGMLLPMGYGQGPGQAYN
                     WAHTYETSWGFANGTSFGLTVSSVGFIACSIGGITFLNLMRLRRNPKATSRIGAELRD
                     EVRPEDIAAPNEVPLSDTLDKMTLQFGLVFLTYALAFGIIYALSALCDLSGVELLTST
                     VKPLIWGFNFIFAALAALLVQGSIGFLTK"
BASE COUNT          267 a          445 c          410 g          267 t
ORIGIN      
        1 ggagcttgcc gacgtgccgc tgatcctgcg cgaggaagga agcggcgccc gggagtattt
       61 caagcggatg atgcagtccg cgggcatacc cttcacggtc cgctggagca gctacagcca
      121 tagcgcgatc atcgacgcgg tcgagcacga cctgggcgtc ggcgtcatct ccggcatgct
      181 ggccaggaag tactcaggcg gcggcaggct ccatatatgc gagatagccg acgtcgagct
      241 cgaccgtagc ttcaagctgg tctacaagaa gaacaagttc atcacgaaca tcctgatgag
      301 gttcacgcag atatgcggga acacgaagta cttcaatatg gccccggagc agctgtagcg
      361 ggctcggatc gcgggagcat aaggagatgc ctatacatcc catagaataa ttatatttcc
      421 cctcctggaa atgcgatgct acgctgaggc ccggacgcaa tggaggccga agcgatgcaa
      481 tcaagctaca acctatggga tccgggagta tggtccttcg tattcgcgct cgcgatcctc
      541 ttcctcggga tgatcgccgc gaacgccctg atccgactcg tcaagcccct ccggaacatg
      601 ctgataccga gctcggtatt gggagggttc atgctcgtgg ccttcctcgc catcatgaag
      661 gccgttaccg gaaagagcct gatcgagccg tccacgctcg agatgatcac ctaccacggc
      721 ctcggcctgg gctgcaccgc tctggcgctg aaagtggaga agaagacgaa gcgacgccac
      781 gcgcagcgcg acatcttcaa ctcggcgctg gtaaccacct cgggctacct gcttcaggtc
      841 gtgatcggcc tctctgtatc cttcgccctc tacttcgtgc tggccgaggt ctggcccgct
      901 tccggcatgc tgttgccgat gggctacggg caggggcccg gccaggccta caactgggcc
      961 catacctacg agacgagctg gggcttcgcc aacggcacca gcttcggcct taccgtatcg
     1021 tcggtcggct tcatagcctg ttccataggc ggcatcacgt tcctgaacct gatgcgccta
     1081 cggcgtaacc ccaaggccac ctcgcgcatc ggcgccgagc tgcgtgacga ggtgcggccc
     1141 gaggacatcg ccgcgccgaa cgaggtgccc ctgtccgaca cgctcgacaa gatgacccta
     1201 cagttcggcc tcgtcttcct tacctacgcg ctcgccttcg gcatcatcta cgcgctcagc
     1261 gccctctgcg acctgagcgg ggtcgagctt ctgacgagca cggtgaagcc gctgatctgg
     1321 ggcttcaact tcatcttcgc ggcgctggcc gccttgctgg tacagggctc catcggcttc
     1381 ctgaccaag
//
LOCUS       DAOPHV010000717         1388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_138560_length_1388_cov_2.575394, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000717 DAOPHV010000000
VERSION     DAOPHV010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138560_length_1388_cov_2.575394"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..639)
                     /locus_tag="PLQ29_13695"
     CDS             complement(226..639)
                     /locus_tag="PLQ29_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPG87748.1"
                     /translation="MPGISCSWRGDMSFSTVVNGHEIIIDADAEFGGKDSGPRPKPLI
                     LAALAGCSGMDVVSILKKMREPISWFNVRVEADQSDEQPKTYTSMRIIYEFKASDGLK
                     DENVRKAVDLSLERYCGVAALLKKAVPLGSEIAYL"
     gene            complement(717..1238)
                     /locus_tag="PLQ29_13700"
     CDS             complement(717..1238)
                     /locus_tag="PLQ29_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87749.1"
                     /translation="MRRAGIALSCFLALGVAAWADGTRYVYEIRALGSAGTDPWSMNM
                     AVAAEAARAYAPVSFKRTGTAVSFAMDTELSLSLGPESELERSAERVLVRRAVTIRGE
                     RVLPKARKKARIRFSLSDLMAEDGKAARSPLEYALAKAVEASRYASGRAWVVSAEYDG
                     AGRFLVVVALTDR"
BASE COUNT          258 a          463 c          396 g          271 t
ORIGIN      
        1 aagagcccga atgtatgccg cagcacccgg ttgaaccaac ggggggcgcg cggcctgtac
       61 ctagctttgt ctatcatggc gtcctcggta ctactatgag tcgagatcgc cgatactgca
      121 acgtagaaat cgaggcgatc gtggatgaat cgtcagccta tcgcctcagg acgacgatat
      181 cccggttaaa aagcggcggc cgtgcggccg ccgcccctgg cgggcctaga gatacgctat
      241 ctcgctaccg agcggtacgg ccttcttgag cagggcggcc acgccgcagt agcgctcgag
      301 cgagaggtcc accgccttgc gcacgttctc gtccttgaga ccgtccgagg ccttgaactc
      361 gtagataatc ctcatgctcg tataggtctt gggttgctcg tcggactggt cggcctcgac
      421 gcgcacgttg aaccagctta tgggctcgcg catcttcttg aggatcgaca cgacgtccat
      481 gcccgagcag ccggccaggg ccgccaggat caatggcttg ggcctcggcc ccgaatcctt
      541 gccgccgaat tctgcgtccg cgtctatgat gatctcgtgt ccgttcacca cggtcgagaa
      601 ggacatatcg ccgcgccatg agcacgaaat acctggcatc gcccacctcc gtcaatgaat
      661 atactcaacc caatatagta tattggcgcg gacgattata gtctcagcgc gcggctttag
      721 cggtcggtca gcgccacgac gaccaggaag cgccccgcgc cgtcgtactc cgcggagaca
      781 acccaggccc tgccgctcgc gtagcgcgac gcctcgacgg ccttcgccaa ggcgtactcg
      841 agcggggaac gggccgcctt gccgtcctcg gccatcaggt cggacaagct gaaccgtatc
      901 ctggccttct tccgcgcctt gggtagcacc cgctcgcctc ggatcgtcac agcgcgccgt
      961 accaacaccc gctccgccga gcgttccagc tccgactccg gtccgagcga cagcgacagc
     1021 tccgtgtcca tcgcgaacga gaccgccgtc ccggtcctct tgaacgatac tggcgcgtac
     1081 gcccgcgccg cctccgccgc tacggccatg ttcatcgacc acgggtcggt cccggcgctc
     1141 ccgagcgccc tgatctcgta tacgtagcgc gtaccgtccg cccatgccgc gactccgagc
     1201 gcgaggaagc acgatagggc aatgccggct cttctcatac gaagctcctt ccaggactcg
     1261 ttcctaacca taatgatagc gtatcggcac ttgattaaac gccttgtttt cgctatagtc
     1321 gcattcgctt aacggggaat tagctcagtt ggtagagcga taggttcgca atctataggt
     1381 caggggtt
//
LOCUS       DAOPHV010000718         1387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_138638_length_1387_cov_4.617868, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000718 DAOPHV010000000
VERSION     DAOPHV010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138638_length_1387_cov_4.617868"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PLQ29_13705"
     CDS             complement(<1..724)
                     /locus_tag="PLQ29_13705"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87750.1"
                     /translation="MSIATRCRFRFDTIHDAGGTKTAMKLSAALASAAALAALAAAFL
                     SSEPASALRAFFLAPFRTRTAFLSTLELAAPLAVCALGVIVTFRAGHYSLGGEGQAYA
                     GAFAAALAAPILYESLGPASAPLAILVGALAGAIVSAPSAIGKRLAGADVLLTSILVS
                     QGVIFAVDWAIGGPFRDQSSNLVAMRAIPRGALLPRLAPPSTLTAASIVALAACLIAA
                     FYFERTRSGTAHDLYGRNQRFAE"
     gene            complement(721..>1387)
                     /locus_tag="PLQ29_13710"
     CDS             complement(721..>1387)
                     /locus_tag="PLQ29_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG87751.1"
                     /translation="LTVRSGEIVSVVALGGNGLESLEDIAAGVVGPDEGDVLVKGRPL
                     AKWPRRELRSRVMAYLPTDRENRGLSPKASVAMNAVAGRLFDYSFAEFAAGSSPLTDA
                     GRMLGEFGVANWKGRRVDTLSGGNRQRVVASRELSGDAELVVAANPAQGLDRGSRSRL
                     LSRLAAMRDSGSAVLLLSSDPDDAAELADRSFALYRGRLTPIERTGSFDAAFSEALTG
                     AKP"
BASE COUNT          235 a          473 c          497 g          182 t
ORIGIN      
        1 gctcggcgaa gcgttggtta cgtccgtaga ggtcgtgggc cgtcccgctc cgcgtacgct
       61 cgaagtagaa agccgcgatg aggcaggcgg ccagggcgac gatcgaagcc gccgtcaggg
      121 tcgacggcgg agcgagcctc ggtagtaggg cgccgcgcgg tatggcccgc atcgccacca
      181 ggttgctcga ctggtcgcgg aacggcccgc ctatggccca atccaccgcg aagataacgc
      241 cttgggagac tagaatgctc gtcaggagca cgtcggcccc ggccaggcgc ttgcctatgg
      301 ccgagggagc cgatacgatg gcgcctgcca gggcgccgac caggatcgct agcggcgccg
      361 aagccggccc cagggactcg tacaagatgg gcgcggccaa ggccgcggca aacgctccgg
      421 cgtaggcctg gccctcaccc ccgaggctgt agtgaccggc gcggaacgtg acgatcacgc
      481 cgagcgcgca gacggcgagg ggggcggcca gctccaaggt ggacaggaac gcggtcctgg
      541 ttctgaaggg cgccaagaag aaagcgcgca gggccgaggc tggctcgctt gataggaatg
      601 ccgccgccag ggccgcgagg gcggccgcgc tggcgagcgc ggcgcttaat ttcatagcgg
      661 tcttggttcc gccggcgtca tgaatcgtgt cgaaacggaa tcggcaacgg gtggcgatgc
      721 tcacggcttc gcccccgtca aggcctcgga gaaggcggcg tcgaacgagc ccgtacgctc
      781 gatcggcgtc agccgccccc tatacagggc gaacgagcgg tcggcgagct cggcggcgtc
      841 gtcggggtcg gagctcagga gcagtaccgc cgagccggag tcgcgcatcg ccgccagccg
      901 cgagagcagc cgcgagcggg agccgcgatc caggccctgg gcagggttag ccgccacgac
      961 gagctcggcg tctccggaca gctcgcgcga cgcgaccacg cgctgccggt tgccgccgga
     1021 cagcgtgtcg acgcgcctgc ccttccagtt ggccacgccg aactcgccga gcatgcgacc
     1081 cgcgtcggtc aacgggctcg agccggccgc gaactcggcg aacgaataat cgaataggcg
     1141 gccagccacg gcgttcatcg ctacgctggc tttcggggat agaccgcggt tctccctgtc
     1201 ggtgggtaga tacgccatca ccctcgagcg aagctcccgc ctcggccatt tcgccagggg
     1261 ccggcccttg accagcacgt ctccctcgtc cgggccgacg acgccagcgg cgatgtcctc
     1321 gagcgactcc agcccgtttc caccgagggc cacgaccgac acgatctcgc ccgagcggac
     1381 ggtcagg
//
LOCUS       DAOPHV010000719         1386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_138827_length_1386_cov_3.537941, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000719 DAOPHV010000000
VERSION     DAOPHV010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138827_length_1386_cov_3.537941"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1386)
                     /locus_tag="PLQ29_13715"
     CDS             complement(<1..>1386)
                     /locus_tag="PLQ29_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HPG87752.1"
                     /translation="YREFGGKVSAVAVKAFAILVAFYHLYGAVFGTPVTLKHRSLHVA
                     MILALGFIFYPFRKKGNRSKVAIPDLVLALLAAATTVYVIVDYIGIVGRAGMPNLTDL
                     VFAALLILLVLEGARRVTGWALPALGVVFILYALFGRGLPGFFGHRGYSWADLSSFLF
                     VSTDGVYGTAVGVSASYIYLFILFGAFMDKSGMGQFINDIALALAGHQKGGPAKVSVI
                     ASALLGTINGSAVANVVTTGTFTIPLMKKTGYDKDFAGAVEASSSVGGQLMPPIMGAA
                     AFIMAEVLGIQYKVIAVAAILPALLYYVGIIVQVHLRARKCGLEGVSRENLPKAWAVL
                     KERGHLLLPIVFLLVMLFASGKTVIFSAFMTILVTIATSMLKKSTRMSLKDILDALFE
                     GTRSMVPVAIACASVGLVIGVASLTGFGLSMASAIVALGGKSLFLTLAFTMVASMILG
                     MGLPSIQATIVK"
BASE COUNT          283 a          425 c          468 g          210 t
ORIGIN      
        1 cttcacgatg gtcgcctgta tgctcggcag gcccatgccc aggatcatgg aggcgaccat
       61 cgtgaaggcg agcgtcagga acaggctctt gccgccgagg gcgacgatgg cgctggccat
      121 cgacaagccg aagccggtca ggctcgccac gccgataacg aggccgacgg aggcgcaggc
      181 tatcgctacg ggaaccatcg acctggtacc ctcgaacagc gcgtcgagga tgtccttgag
      241 gctcattctg gtggacttct tgagcatcga cgtcgcgatg gtgacgagta tggtcatgaa
      301 ggccgagaag ataaccgtct taccggaggc gaagagcatg accagcagga acacgatggg
      361 caggagcaga tggccgcgct ccttgagcac agcccaagcc ttcggcaggt tctcgcgcga
      421 tacgccttcg agcccgcact tccgggcccg caggtgcacc tgcacgatga tgccgacata
      481 gtacaggagg gccggcagga tggccgcgac ggctatgacc ttgtattgga tgccgagcac
      541 ctccgccatg atgaaggcgg ccgcccccat gatcggcggc atcagctggc ctccgaccga
      601 ggacgaggcc tcgaccgcgc cggcgaagtc cttgtcatag ccggtcttct tcatgagcgg
      661 gatcgtgaag gtgcccgtgg tgacgacgtt cgctaccgcc gagccgttga tcgtaccgag
      721 gagggcgctc gcgatgaccg acaccttagc cggcccgccc ttctgatggc cggccagggc
      781 caaggcgatg tcgttgatga actgccccat gccggacttg tccatgaagg cgccgaacag
      841 gatgaacagg tagatgtacg aggccgacac gccgacggcc gtgccgtaga cgccgtcggt
      901 cgagacgaac aggaagctcg acaggtcggc ccatgaataa ccgcggtggc cgaagaagcc
      961 gggcaggccg cgaccgaaca aggcgtacag gatgaagacc acgccgagcg ccggcagggc
     1021 ccagccggtc acgcgcctgg cgccctcgag cacgagcagt atcagcaggg cggcgaagac
     1081 gaggtcggtc aggttgggca taccggcgcg gccgacgatg ccgatatagt cgactatcac
     1141 gtagaccgtg gtcgccgcgg cgagcagcgc gagtaccagg tcgggtatgg cgaccttgct
     1201 acggttcccc ttcttcctga acggatagaa gatgaacccg agcgccagta tcatcgccac
     1261 gtggagcgag cggtgcttga gggtcaccgg cgtgccgaaa acggcgccgt acaggtggta
     1321 gaacgccacg agtatcgcga acgccttgac ggctacggcg ctgaccttgc cgccgaactc
     1381 gcggta
//
LOCUS       DAOPHV010000720         1385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_138967_length_1385_cov_4.550376, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000720 DAOPHV010000000
VERSION     DAOPHV010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138967_length_1385_cov_4.550376"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..>1385
                     /locus_tag="PLQ29_13720"
     CDS             127..>1385
                     /locus_tag="PLQ29_13720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPG87753.1"
                     /translation="MNAVIETKPVQGDDGACNQGGPDACCAACAAHERARAEAFSGDA
                     EPRGHGLSAGTRREIATLTVSALLAVAGYVAQAASAESAGGPARWIALALFAVAYLLA
                     GRDVLASAARNIARGKVFDELFLMSIASLGAFAIGAMEEAIGVMIFYKVGEMLQESAA
                     DKSRASIRSLLAMRPDKARVRRGGDWVECRPEDVAPGDLVLVRPGERVPVDGVVTDGA
                     GAFDTSSMTGESAPRPAGPGAEALSGFIAVEAALTIRAERVASESSAARVVELVENAS
                     KAKAKTELFITRFARWYTPAVVAVALLVAFGPPLLLPGQRLADWAYRALVMLVISCPC
                     AFVISVPLGYFGGLGGAARRGILIKGASVLDALADAKTVVFDKTGTLTDGSFSVRELS
                     PEGGFSDEDLLGGAVAAETHSNHPLATA"
BASE COUNT          202 a          470 c          512 g          201 t
ORIGIN      
        1 acgccgctcc agactggtac gctccaggcg cgacggcaag tcggtgttct atgcgctcga
       61 cgacggacac gtgggccaga tcgtcggcat cgccaccgag cacgtggccg agaggcggga
      121 ggaaccatga atgcggtcat cgagacgaag ccggttcagg gcgacgatgg ggcctgcaac
      181 cagggcggcc cggacgcctg ctgcgcggcc tgcgcggccc acgagcgggc cagggccgag
      241 gccttctcgg gcgacgcgga gcctcgtgga catggattgt ccgcgggaac gaggcgcgag
      301 atagcgacgc tgaccgtatc ggccctactg gcggtcgccg gctacgtcgc gcaggccgcc
      361 tcggcggaga gcgcgggcgg tccggcgcgc tggatagccc tcgcgctctt cgccgtagcc
      421 tacctgctgg caggccgcga cgtgctcgca tcggccgcca ggaacatagc ccggggcaag
      481 gtcttcgacg agctgttcct gatgtccata gcctcgctcg gcgccttcgc catcggcgcc
      541 atggaggagg cgataggcgt catgatcttc tataaagtcg gcgagatgct ccaggagagc
      601 gccgccgaca agagccgcgc ctcgatacgc tcgctcctgg cgatgcgccc cgacaaagcg
      661 cgcgtcaggc gcgggggcga ctgggtcgaa tgccggcccg aggacgtggc gcccggcgac
      721 ctggtgctgg tcaggccagg cgagcgcgtg ccggtcgacg gtgtcgtgac cgacggagcc
      781 ggggccttcg acacctcgtc gatgaccggc gagagcgcgc cgaggccggc gggtccaggc
      841 gccgaggcgc tgtcgggctt catcgcggtc gaggccgcgc tgacgatacg ggcggagcgc
      901 gtagcctcag agtcatcggc ggcgcgcgtc gtcgagctgg tggagaacgc ctccaaggcc
      961 aaggcgaaga ccgagctgtt catcacgcgc ttcgcccgct ggtacacgcc ggcggtcgtc
     1021 gcggtcgccc tgctcgtcgc gttcggtccg ccgctgctgc tacccgggca gcgcctggcg
     1081 gactgggcct accgagcgct cgtgatgctg gtcatctcct gcccctgcgc cttcgtcatc
     1141 agcgtgcctc tcggctactt cggcggcctg ggtggcgcgg ccaggcgcgg catcctgatc
     1201 aagggcgcct cggtgctcga cgcgctggct gacgccaaga ccgtagtctt cgacaagacc
     1261 ggcacgctga ccgacgggag cttcagcgtc cgcgagctgt cgccagaggg cggcttcagc
     1321 gatgaggacc tgctcggcgg ggcggtcgcg gccgagacgc actcgaacca cccgctggcg
     1381 acggc
//
LOCUS       DAOPHV010000721         1384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_139100_length_1384_cov_4.245297, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000721 DAOPHV010000000
VERSION     DAOPHV010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139100_length_1384_cov_4.245297"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1384)
                     /gene="glmS"
                     /locus_tag="PLQ29_13725"
     CDS             complement(<1..>1384)
                     /gene="glmS"
                     /locus_tag="PLQ29_13725"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPG87754.1"
                     /translation="RDEPLKDGEAPLSVIKKTGKIAELRQAVPKDLPGTCGIGHTRWA
                     THGGVTDANAHPHVDGSGRIAIVHNGIIENSGSLKEMLEKEGARFASETDSEVIAHLV
                     AYYYDGNLETALKAALGHLRGTYGIAAIHADEPGRIVGARNGSPLVVGVGDGEMLLAS
                     DVTALLAHTASVVYMNDGEVVSMTPAEFRISDGDDRAVDPKIDTITWKLDAIEKEGFS
                     SYMEKEIFEQPESIDRALTGRMDLEYGSAKLGGLNLSKKELHAIERVRVIAAGTSWHA
                     GLVGCQLLESVARLPAQAELASELRYRNPVVEPDSLYLAVSQSGETADTLYAMREIQR
                     RGGSVLGVCNVVGSTIARESDGGIYVHSGPEIAVASTKAFTSQLAAFYLLTLSLARLH
                     DMSHHEGQRFLAELRAIPEAVRACLERRDAIQAIAKRYARYRDFLFLGRGLLYPIALE
                     GALKLKEISY"
BASE COUNT          193 a          451 c          492 g          248 t
ORIGIN      
        1 atgtacgaaa tctccttgag cttgagcgcg ccctcgaggg ctatcgggta caggaggcca
       61 cgcccgagga acaggaagtc gcggtagcgg gcgtaacgct tggcgatggc ctggatcgcg
      121 tcgcgccgct cgaggcaggc gcgcaccgcc tcgggtatag cgcgcagctc cgccaggaag
      181 cgctgcccct cgtggtgcga catgtcgtgc aggcgggcca gcgacagggt caggaggtag
      241 aaggcggcga gctggctcgt gaaggccttg gtcgacgcga cggctatctc cgggccggag
      301 tgcacgtaga tgccgccgtc gctctcgcgg gctatggtcg agccgacgac gttgcagacg
      361 ccgagcaccg agccgccgcg gcgctggatc tcgcgcatcg cgtagagcgt gtcggcagtc
      421 tcgccggact gcgagaccgc gaggtacagg gagtcgggct cgaccaccgg gttgcggtag
      481 cgcagctcgc tggccagctc ggcctgggcc ggcaagcgcg ccaccgactc caggagctgg
      541 cagccgacga ggccggcgtg ccagctcgtg ccggccgcta tgacgcgcac gcgctcgatc
      601 gcgtgcagct ccttcttgga cagattgagg ccgccgagct tggccgagcc gtactccagg
      661 tccatgcggc cggtcagggc gcggtctatg gactctggct gctcgaagat ctccttctcc
      721 atgtacgacg agaagccttc cttctcgatc gcgtcgagct tccaggtgat cgtgtctatc
      781 ttcgggtcga cggcgcggtc gtcgccgtcg gatatcctga actcggccgg cgtcatcgac
      841 accacctcgc cgtcgttcat gtagacgacg ctcgcggtat gggccaggag cgcggtcacg
      901 tcgctcgcga gcagcatctc gccgtcgcct acgccgacga cgagggggct gccgttgcgg
      961 gccccgacga tacggccggg ctcgtcggcg tggatggcgg ctatgccgta ggttccgcgc
     1021 aggtgtccga gggccgcctt gagcgccgtc tccaggttgc cgtcgtagta gtaggcgacg
     1081 aggtgggcga tcacctcgct gtcggtctcg ctcgcgaagc gcgcgccctc tttctcgagc
     1141 atctccttga gggagcccga gttctcgatg atgccgttat ggacgatggc tatcctgccc
     1201 gagccgtcga cgtggggatg ggcgttcgcg tcggtcaccc cgccgtgggt ggcccagcgc
     1261 gtatggccga tgccgcacgt tccaggcagg tccttcggca cggcctgcct gagctcggct
     1321 atcttgccgg tcttcttgat gacggacagg ggggcttccc cgtccttgag cggctcgtcc
     1381 ctgc
//
LOCUS       DAOPHV010000722         1383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_139336_length_1383_cov_2.528614, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000722 DAOPHV010000000
VERSION     DAOPHV010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139336_length_1383_cov_2.528614"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="PLQ29_13730"
     CDS             complement(<1..1137)
                     /locus_tag="PLQ29_13730"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPG87755.1"
                     /translation="MASDAQKKVLVLNSGSSSLKYEVFLMPKRESLGKGLVERIGEAK
                     GYLTQSSPKGSIRIDEVFPDHKHAMLRVGQALVDPLKGILGSIDEIEAIGHRVVHGGE
                     RFASSVRIDEAVVKALEDNVELAPLHNPANLTGIAEAEQLWPGVPQVAVFDTAFHQTM
                     APTAYLYGLPRELYDKYRIRRYGFHGTSHRFVARRALELLGRRAENTNLITCHLGNGA
                     SITAIEGGRSIDTSMGFTPLEGLMMGTRSGDFDPAILGFLVDRGYSPQELTTMINKKS
                     GLLGLSGISNDLRDLEAAAEKGNRNAVEALDVYAHRVRQYIGAYMAELVKVDAIVFTG
                     GVGQHGVKMRERICHRLENIGIMMDYDANRDDGSAEGAVSQPYSP"
BASE COUNT          244 a          381 c          455 g          303 t
ORIGIN      
        1 gggcgagtag ggctgcgaca cggctccctc ggctgagccg tcgtcgcgat tggcatcgta
       61 gtccatcatg atgccgatgt tctcgagccg gtggcaaata cgttctcgca tcttgacgcc
      121 gtgctggccg acgccgccgg tgaacacgat ggcgtcgacc ttgacgagct cggccatgta
      181 cgccccgatg tactggcgaa cgcggtgagc gtagacgtcg agcgcctcga cggcgttgcg
      241 gttgcccttc tcggcggcgg cctccaggtc gcgcaggtcg ttggagatgc cggagaggcc
      301 gaggaggccg ctcttcttgt tgatcatcgt cgtcagttcc tgaggcgaat agccgcgatc
      361 gacgaggaag cccaagatgg ccgggtcgaa gtcgccggag cgggtgccca tcatcagtcc
      421 ctcgagcggc gtgaagccca tggacgtgtc gatggagcgg ccgccttcga tggcggtgat
      481 cgacgcgccg ttgccgaggt ggcaggtgat caggttggtg ttctcggctc tgcggccgag
      541 tagctcgagg gcgcgcctgg ccacgaagcg gtggctcgtg ccgtggaagc cgtagcgcct
      601 gatacggtac ttgtcgtata gctcgcgggg taggccgtac aggtatgccg tgggcgccat
      661 ggtctggtgg aatgcggtgt cgaagacggc gacctgaggg acgccgggcc atagctgctc
      721 ggcctcggct ataccggtca ggttggcggg attgtggagc ggcgccagtt cgacgttgtc
      781 ttcgagcgcc ttgacgacgg cctcgtcgat acggacggag cttgcgaagc gctcgccgcc
      841 gtgcacgacg cggtgtccga tggcctcgat ctcgtcgatg gagccgagta tgcccttgag
      901 cgggtctacg agggcctgcc cgacgcggag catcgcgtgt ttatggtcgg ggaacacctc
      961 gtcgatcctg atcgagcctt taggcgaact ctgggtcaag tagcctttgg cttcaccgat
     1021 gcgctcgacg agtcccttgc cgaggctttc acgctttggc atgaggaaca cctcgtattt
     1081 gagcgatgag ctgccgctat tgagcacgag cactttcttc tgagcgtctg aagccattat
     1141 tattcgtccc ttccgtcggt cgatgcctga tgaacgggta gaaggccgac gaatggatca
     1201 taagccaata tgatcgtttc atcaatcgat atcatctgta gagatatgaa ccgtattgac
     1261 gaatcaagtc cgtgtttgct gttcggacta acctctggaa tctccgaccg tgacctgccg
     1321 ttatgatgaa agcaattctt accaaaataa gaattagttg catgaggacc actcagcttt
     1381 gtt
//
LOCUS       DAOPHV010000723         1382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_139491_length_1382_cov_2.792766, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000723 DAOPHV010000000
VERSION     DAOPHV010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139491_length_1382_cov_2.792766"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLQ29_13735"
     CDS             complement(<1..611)
                     /locus_tag="PLQ29_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPG87756.1"
                     /translation="MSLVFEKRKSVSRTAIVVVPVVSFMASLALTGALLMIFGADPLA
                     TYASMAKGAFGSARAFTETLVKAIPLMLTGLGVALAFKLRFWNIGAEGQLTLASIAAT
                     WVALYWNWLPGPLVLPVLVLVGCAAGALWAGVPALLKTRLNVDETLVTLMLNYVALLL
                     AEFLYYGPWRDPKGFGFPGTMVFPKEAWFPRIAGRAHIGLYFA"
     gene            complement(608..>1382)
                     /locus_tag="PLQ29_13740"
     CDS             complement(608..>1382)
                     /locus_tag="PLQ29_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG87757.1"
                     /translation="GAVVLELKDVCADDERGLPALRELSLSVRAGEIVGIAGVAGNGQ
                     RELSEVITGLRPAKSGGVSINCVDLTNRTPLEIIKSGVSHIPSDRIAVGAVGDMSVAS
                     NLAMKDYRAAPISKGALLLPRRILEFARKMIGLFNIATPSPETRVKFLSGGNIQKTIL
                     AREIESCGCLLVAVYPSRGLDIGATESVRKELVAQRDAGKAVLLVSEDIDELLTVADT
                     IAVMYDGRVVGSMPAAGADPEEIGLLMAGVAPSAEEVRA"
BASE COUNT          232 a          468 c          470 g          212 t
ORIGIN      
        1 atggcgaagt acaggcctat atgcgcgcgg ccggctatgc gcgggaacca tgcctccttg
       61 gggaagacca tcgtgcccgg gaagccgaag cccttggggt cgcgccaggg gccgtagtac
      121 aggaactccg cgaggagcag ggccacgtag ttgagcatca gggtgacgag ggtctcgtcg
      181 acgttgagac gcgtcttgag caacgccggc acgccggccc agagcgcccc ggccgcgcag
      241 ccgaccagga ccagcaccgg cagcacgagc gggccgggca gccagttcca gtacagggcg
      301 acccaggtgg ccgcgatgct cgcgagcgtc agctggccct ccgcgccgat gttccagaag
      361 cgtagcttga acgcgagcgc cacgccgagg cccgtcagca tcagcggtat cgccttgacg
      421 agcgtctcgg tgaacgcgcg ggcagatccg aaggcgccct tggccatcga cgcgtaggtg
      481 gccagcgggt ccgccccgaa tatcatgagc agcgcgccgg tcagcgcgag cgacgccatg
      541 aacgacacga cggggacgac gacgatggcc gtcctcgata ccgacttccg tttctcgaat
      601 accaggctca tgccctcacc tcctcggccg acggggcgac gcccgccatg agaaggccta
      661 tctcctcggg atcggccccg gccgccggca tcgagccgac gacccggccg tcgtacataa
      721 cggctatcgt atccgccacg gtcagcagct cgtcgatgtc ctcggatacg agcagtacag
      781 ccttgcccgc gtcgcgctgg gcgaccagct ccttgcgtac cgactcggtg gcgcctatgt
      841 ctaggccgcg ggacgggtag acggccacga gcaggcagcc gcacgactcg atctcgcgag
      901 ccaggatggt cttctggatg ttgccgccgg acaggaactt gaccctggtc tcaggcgacg
      961 gggtggctat gttgaacagc cctatcatct tgcgcgcgaa ttcgaggatg cgcctcggca
     1021 ggagcagcgc gcccttcgag atgggcgccg cgcggtagtc cttcatcgcc aggttgctgg
     1081 cgacgctcat gtcgccgacg gctccgacgg cgatgcggtc ggacgggata tggctgacgc
     1141 cgctcttgat gatctccagc ggcgtgcggt tggtcaggtc gacgcagttg atcgacacgc
     1201 cgcctgactt ggccggccga aggcccgtga tcacctcgga cagctctcgc tggccgttgc
     1261 cggcgacgcc ggctatgccg actatctcgc ccgcgcgcac gctgagcgac agctcgcgga
     1321 gggccgggag gcctcgctcg tcgtcggcgc atacgtcctt aagctcgagc acgaccgcgc
     1381 cg
//
LOCUS       DAOPHV010000724         1377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_140343_length_1377_cov_2.034039, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000724 DAOPHV010000000
VERSION     DAOPHV010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140343_length_1377_cov_2.034039"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PLQ29_13745"
     CDS             <1..728
                     /locus_tag="PLQ29_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPG87758.1"
                     /translation="RRAVAENPANAAMLFVPPRFTKDAVFEALDSGIEKIVTIADGIP
                     LQDAIQIRKAALSCGARVVGGNTSGVISPGLAMMGMFPYWIDRVYKSGRVGVMTRSGS
                     LTNEVTAMVVSAGFGVSSLIGVGGDPVPGTRFAEMLPDYQADPDTDAVVIIGELGGTM
                     EEEVADAMLSGAFTKPVVAFLGGRTAPAGKRMGHAGAIVSGGKGSVKDKIEAFEKAGA
                     LVADRPRMVGELLRTALGETHKR"
     gene            742..>1377
                     /locus_tag="PLQ29_13750"
     CDS             742..>1377
                     /locus_tag="PLQ29_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPG87759.1"
                     /translation="MATLQAKNTTVRNGVILAVALAIGLAVSLTPAPASLVSTIVNAV
                     DPGTLKPSYPASAHSGGPGMAALGVMAFALMLWITEALPFHITGLMAMIFLTLLKVGK
                     YKEIISTGFGNDIVVFFIGVLVITAFITKSGLGKRISAVILSITGNSSRAILFGFLAA
                     GAVLSMWITDMAVAVMLMPLARAILQEEGCKPKESNFGKALMISVAWGPLIG"
BASE COUNT          245 a          451 c          449 g          232 t
ORIGIN      
        1 tacgccgagc cgtagcggag aacccggcca acgccgcgat gctgttcgtg ccgccgcgct
       61 tcacgaagga cgcggtcttc gaggcgctcg actcgggtat cgagaagatc gtgacgatag
      121 cggacggcat accgctccag gacgcgatcc agatccgcaa ggcggccctg tcatgcggcg
      181 cgcgcgtcgt gggcggcaac acctccggcg tcataagccc gggcctggcg atgatgggga
      241 tgttccccta ctggatagac cgcgtctaca agagcggccg cgtaggcgtg atgacgcgct
      301 ccggctcgct gaccaacgag gtcacggcca tggtggtcag cgccggcttc ggcgtgtcca
      361 gcctcatagg cgtcggcggg gatcccgtgc cggggacccg cttcgccgag atgctgccgg
      421 actaccaggc cgaccccgat accgacgccg tcgtcatcat cggcgagctc ggcggaacca
      481 tggaagagga ggtggccgac gcgatgctct ccggcgcctt cacgaagccg gtcgtcgcgt
      541 tcctcggcgg acggaccgct ccggcgggca agcgcatggg ccatgccggc gccatcgtct
      601 caggcggcaa gggctcggtc aaggacaaga tcgaggcgtt cgagaaggcc ggcgcgctcg
      661 tcgccgatag gccgcgcatg gtcggggaac tgctcaggac cgcgctcggc gagactcaca
      721 agcgataagg acaggaactc aatggcaact ctacaagcaa agaacacgac ggttcggaac
      781 ggcgtcatac tcgcggtcgc gctggccata ggcctcgccg tgtcgctgac gccggcgcct
      841 gcgtctctcg tatcgaccat cgtcaacgcc gtggacccag gaaccctgaa accgtcctat
      901 ccggcgtccg cccactccgg aggcccgggt atggccgcgc tcggcgtcat ggccttcgcc
      961 ctgatgctct ggatcaccga ggccctgcca ttccatatca ccggcctcat ggcgatgata
     1021 ttccttaccc tgctgaaggt aggcaagtac aaggagatca tctcgacggg cttcggtaac
     1081 gacatcgtcg tgttctttat cggcgtgctc gtcataaccg cgttcataac caagtcgggc
     1141 ctcgggaagc gcatctcggc ggtcatcctg tcgataacgg gcaactcgag ccgggccata
     1201 ctgttcggct tcctcgcggc gggcgccgtg ctgtccatgt ggatcaccga tatggccgtc
     1261 gcggtcatgc tcatgcccct cgccagggcc atactccagg aggaaggctg caagcccaag
     1321 gagtcgaact tcggcaaggc cctgatgata tcggtagcgt gggggccgct catcggc
//
LOCUS       DAOPHV010000725         1374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_140749_length_1374_cov_3.509477, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000725 DAOPHV010000000
VERSION     DAOPHV010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140749_length_1374_cov_3.509477"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1374
                     /locus_tag="PLQ29_13755"
     CDS             <1..>1374
                     /locus_tag="PLQ29_13755"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPG87760.1"
                     /translation="FDEARHLTNLSLSLYGQEMDNATAALAKMNMILHDAPEAEIWKD
                     NTLSSPHWKSRTGGLLTHDFVVANPPFSTKAWANGIDPLNDQYRRFEYGIPPEKNGDY
                     AFLLHVLASLKPTGKGAIILPHGVLFRGGAEAAIRTSILKQGYIKGIIGLPANLFYGT
                     GIPACIIILDKEGAVGRKGVFLIDASKGFIKDGPKNRLRAQDIHKIVTTFNEGVELER
                     YSRMVPLSEIEANEYNLNLPRYIDSSDPEDAQDIDAHLRGGIPNRDLDDLAAYWNVFP
                     GIRKALFKRGDRPGYSNLKIPQAELRKTIFDDPEFTAFNESTMALFSEWAALNRKKLR
                     AVKAGTPPKTLIDELSEDLLAAFREARLIDAYDVYQHLLNYWEGTMQDDAYQISQLGW
                     QPVVDGGPNVDLLPPELMIRKFFGAEAEAIEALESERDELSRQIEEVCEEHGGDGSVE
                     RQERGEN"
BASE COUNT          295 a          480 c          401 g          198 t
ORIGIN      
        1 ccttcgacga ggccaggcac ctgaccaatc tatctctgag cctctacggg caggagatgg
       61 acaacgccac ggccgccctg gccaagatga atatgatact ccacgacgcc ccggaggccg
      121 agatctggaa ggacaacacc ctctcgtcgc cccattggaa aagccggacg ggcgggctcc
      181 tgacccatga cttcgtggtg gccaatcccc cgttcagcac caaggcctgg gccaacggca
      241 tcgaccccct gaacgaccag taccgtcgct tcgagtacgg catcccgccg gagaagaacg
      301 gcgactacgc cttcctgctc cacgtgctgg ccagcctcaa gcccacgggc aagggcgcca
      361 tcatcctgcc ccacggggtg ctcttccgcg gaggcgcaga ggccgcgatc cggaccagca
      421 tactcaagca gggctacatc aagggcatca tcggcctgcc ggccaacctc ttctacggca
      481 ccggcatccc ggcctgcatc atcatactcg acaaggaagg cgccgtcggc cgcaagggcg
      541 tcttcctgat cgacgccagc aagggcttca tcaaggacgg ccccaagaac cggctccggg
      601 cccaggacat ccacaagatc gtcaccacct tcaacgaggg cgtcgagctg gagcgctact
      661 cccgcatggt gcccctgagc gagatcgaag ccaacgagta caacctcaac ctcccccggt
      721 acatagattc cagcgacccg gaggacgcgc aggacataga cgctcacctg cggggcggca
      781 tacccaaccg cgacctcgat gacctagccg cctattggaa cgtcttcccc ggcatccgca
      841 aggcgttgtt caagagaggg gatcgcccgg gctattccaa tcttaagatc ccccaggccg
      901 agctcaggaa gaccatcttc gacgacccgg agttcaccgc gttcaacgag tcgacgatgg
      961 cgctcttctc ggaatgggcc gcgctcaaca ggaagaagct cagggccgtc aaggccggca
     1021 cgcctcccaa gacgctcatc gacgaattga gcgaggatct gctcgccgcc ttccgcgagg
     1081 cccggctcat cgacgcctac gacgtgtacc agcacctcct caactactgg gaaggcacga
     1141 tgcaggacga cgcctaccag atcagccagc tcggctggca gcccgtcgtc gacggcggcc
     1201 ccaacgtcga cctccttccc cccgagctga tgatacgcaa attcttcggc gccgaggccg
     1261 aggcgataga agccttggag tccgagcggg atgagctgag ccgacagatc gaggaggtct
     1321 gcgaggagca cggcggcgac ggctccgtag agcggcagga gcgcggcgag aacg
//
LOCUS       DAOPHV010000726         1356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_143661_length_1356_cov_2.592621, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000726 DAOPHV010000000
VERSION     DAOPHV010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143661_length_1356_cov_2.592621"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PLQ29_13760"
     CDS             complement(<1..256)
                     /locus_tag="PLQ29_13760"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HPG87761.1"
                     /translation="MKAQLLRLVLILFGCTVVIVPVAIVLSRSLTKPIERISAVFREI
                     SSGELTDLSRSLDARAGDEIGEMSRVVNETFERIRGLVAAI"
     gene            492..>1356
                     /locus_tag="PLQ29_13765"
     CDS             492..>1356
                     /locus_tag="PLQ29_13765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG87762.1"
                     /translation="MINKKASLPLLFSVVFMDMVGFGFIIPLIPDYIRAFGGAPSLAG
                     VLMASYALGQFFAAPIVGRLSDRWGRKPLFLFSIGGTFLSLLLLGSARSLPLIFASRI
                     LDGLTGGNITVAQSYIVDITDDRNRAKGLGIIGMAFGFGFIIGPLFGGVLSRFGLSAP
                     AFVAAGIAALNLVLITAILPESLSTERREDLQRNPRRAFSGALLLETLRRKGTGTVLR
                     LSMVYSFAFTMFESMFALSAAEALGVSPSTRGWLLAYIGVLVALVQGGGIGYLAKRFD
                     ERRLGAVAISTV"
BASE COUNT          241 a          427 c          408 g          280 t
ORIGIN      
        1 gaatggccgc tacgaggccg cggatgcgct cgaaggtctc gttgaccact cgggacattt
       61 cgcctatttc gtctccggcg cgcgcgtcca ggctcctcga caggtcggtc agctcgccgc
      121 tcgatatctc cctgaataca gcgcttatac gctcgatcgg cttcgtcagc gaacgcgaca
      181 gcacgatggc gacaggcacg atcacgacgg tacagccgaa caggatcagg acgaggcgca
      241 ggagttgagc cttcataatc gaagcgatgg cgccggcgga aacgtcgagg cacaggagtc
      301 cgtcgaaact cccgatatac aatattcatt ctgatgcgct ttggcttaac caaatggccc
      361 agcgcgcgag tagccctatt cagcggccgg tccgaagccc ttgcccgcgg gcgggcggcg
      421 ctactatctt acaaacgtgc tgcatatcca agggctgttt aaaagtaccg gcacagatca
      481 aggaagcatt gatgatcaat aagaaagcgt ctctgccgct attgttctcg gtcgtcttca
      541 tggacatggt gggcttcggc ttcatcatcc cactgatccc ggactacatc cgcgccttcg
      601 ggggagcccc gtcgctagca ggcgtcctga tggcgagcta cgccctgggt cagttcttcg
      661 ccgctccgat cgtcggcagg ctgtccgacc gctggggccg taagcctctc ttcctgtttt
      721 cgataggcgg caccttcctt tcgctgttgc tgctcggctc cgcgcggtcg ctgccgctga
      781 tattcgcgag caggatcctg gacggcttga ccggcggcaa tatcaccgtg gcccaatcgt
      841 acatcgtcga catcaccgac gacaggaaca gggcgaaggg acttggcatc atcggcatgg
      901 ccttcggctt cggtttcatc atcggtccgc tgttcggagg cgtcctgtca cgcttcggcc
      961 tgtcggctcc ggctttcgta gccgccggca tagctgccct gaacctggta ctgatcaccg
     1021 ccatcctgcc cgagagcctt tccaccgaac ggcgcgagga tctccagagg aatcccagac
     1081 gcgcgttctc cggcgcgttg ctcctggaaa cgctgaggcg aaaagggacc ggcacggtac
     1141 tccgcctgtc gatggtctac tccttcgcct tcacgatgtt cgagagcatg ttcgccctat
     1201 ccgccgccga ggcgctcggc gtaagcccgt cgacacgcgg gtggctcctg gcctatatag
     1261 gcgtgttggt cgccttggtg cagggaggcg gcatagggta cctggccaag cgcttcgacg
     1321 agcgcaggct gggggccgtc gccatatcga cagtgg
//
LOCUS       DAOPHV010000727         1353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_144083_length_1353_cov_5.838213, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000727 DAOPHV010000000
VERSION     DAOPHV010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144083_length_1353_cov_5.838213"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="PLQ29_13770"
     CDS             complement(<1..1149)
                     /locus_tag="PLQ29_13770"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPG87763.1"
                     /translation="MIAPMKRAFVMMMEREKKEALHTLRRLGSLHVEPLEGVGGDYDE
                     LSASVAAMREAIGLLSEFKAEKQLEELNMRDGLDLANRVRRLDAESKAANDEAAATAR
                     EIERIRPWGDFDPSAIAELSGKGIPLRLVELSAKKLEAASFGSDFIRLGEAKGSVRLA
                     VIAGPEAALPSDCVEFRLPAKGLSELEAELIDARSRASAARQAMAAEAPRSEGLKKAL
                     ARVERDMAFEALRSGMSAGDGVSWLAAWVPAKEEKRLSDLAAKEGWGLLLDDPLEEEQ
                     PPTKIENPAIIRMIQPVFDFLGTVPNYREYDISGLFLLFFSVFFAMIFGDGGYGGILL
                     LAGAALAIKSKASGKPVPDFIRLLMVLAATTVVWGALTMSWFGLPPERV"
     gene            complement(1146..>1353)
                     /locus_tag="PLQ29_13775"
     CDS             complement(1146..>1353)
                     /locus_tag="PLQ29_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPG87764.1"
                     /translation="EIKVLEEQIARLSRELRTTTQRVNLFEKVKIPETAASIKKIGIY
                     LGDQQTAQVVRGKMAKRKVERIAS"
BASE COUNT          212 a          467 c          432 g          242 t
ORIGIN      
        1 cacgcgctcg ggcggcaggc cgaaccagct catcgtcagc gctccccaga cgacggtcgt
       61 cgccgcgagc accatgagga gcctgatgaa gtcgggcacc ggcttgccgg aggccttcga
      121 cttgatcgcc agcgcggcac cggccagtag caggatgccg ccgtagccgc cgtcgccgaa
      181 gatcatcgcg aagaaaaccg agaagaacag gaggaacagg ccggagatgt cgtactcgcg
      241 gtagttgggc acggtgccga ggaagtcgaa taccggctgt atcatcctga tgatagccgg
      301 attctctatc ttggtaggcg gctgctcctc ttcgagcggg tcgtcgagga gtagccccca
      361 gccttccttg gcggcgaggt ccgacaggcg cttctcctcc ttggcaggca cccacgccgc
      421 cagccaggat acgccgtcgc cggcggacat gccggagcgc agggcctcga aggccatgtc
      481 gcgctcgacc ctggccagcg ccttcttgag accctcggag cgcggcgcct cggcggccat
      541 cgcctggcgg gccgccgagg ccctcgagcg ggcgtcgatc agctcggcct ccagctctga
      601 aagccccttg gcgggcaggc ggaactcgac gcagtcgctc ggcaaagccg cttcgggtcc
      661 ggcgatcacg gccagcctga ccgacccctt cgcctcgccg aggcggatga agtccgaccc
      721 gaacgacgcg gcctccagct tcttggccga cagctcgacg aggcggagcg gtatcccctt
      781 gccggatagt tccgcgatcg cggacgggtc gaagtctccc cacggcctga tgcgctctat
      841 ctcgcgggcc gtcgccgcgg cctcgtcgtt cgccgccttg gactcggcat cgaggcggcg
      901 cactcgattg gccaggtcga gaccgtcgcg catgttcagc tcctcgagct gcttctcggc
      961 cttgaattcc gacaggagcc cgatcgcctc gcgcatagcc gcaaccgagg ccgacaactc
     1021 gtcataatcc ccgcctacgc cctcgagggg ctcgacgtgg aggctaccga gcctgcgtag
     1081 cgtgtggagc gcctctttct tctcgcgctc catcatcatg acgaaggcgc gtttcatcgg
     1141 ggctatcatg acgctatcct ctcgaccttg cgcttggcca tcttgccgcg gacgacctgg
     1201 gcggtctgct gatcgccgag gtagatgccg atcttcttga tgctcgcggc ggtctccggg
     1261 atcttgacct tctcgaacag gttgacgcgt tgcgtcgtcg tgcgcagttc ccgggataac
     1321 cgggctatct gctcctcgag caccttgatc tcg
//
LOCUS       DAOPHV010000728         1352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_144302_length_1352_cov_3.359291, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000728 DAOPHV010000000
VERSION     DAOPHV010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144302_length_1352_cov_3.359291"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PLQ29_13780"
     CDS             <1..344
                     /locus_tag="PLQ29_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87765.1"
                     /translation="DPAYFSDADFTVVKQKLEDEQIINLETPEHFIETLSFWWSVAST
                     EYFFDYVPSMKKVQRADIGAFLTKFVLSNVPVIAVRLNPSDYATEAGALEAAGFKTVS
                     ADNAFWWKDGE"
     gene            341..>1352
                     /locus_tag="PLQ29_13785"
     CDS             341..>1352
                     /locus_tag="PLQ29_13785"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPG87766.1"
                     /translation="MMTRTYTSRPRAGMVAAAIAAALLAASCAGAPASAPETVTPAAT
                     EAPAVASSRPLPSSPVSGWKLSAEPGKFMRANLTTIETRTLANGVKLIVKKNPANRVY
                     SMKVAFRGGSAMTSAEKAGIEAMTLAMLTRGSSKFSYAAIQRLQYELSSSIGYSASSY
                     DWASFDLNTIDKYWDQMLAVYADCLLNPAFDPAQFSLVQNDFRVSLQKAQADPYSYAV
                     TKLHDSMFAGHPYAADFGGSAASISAISLDDVKAFYRDAIAAERMIVVAVGNFDTDAL
                     AASLNASIGAVPTKGSAIPEVARYDARRSLMLERFDKSKGIGYVRGDFPIATPDSPDF
                     ATL"
BASE COUNT          259 a          469 c          411 g          213 t
ORIGIN      
        1 ccgacccggc ctacttctcc gacgccgact tcacggtggt caagcagaag ctggaggacg
       61 agcagatcat caacctcgag actccggaac acttcataga gacgctgtcg ttctggtggt
      121 cggtcgcctc gaccgagtat ttcttcgact acgtaccgag catgaagaag gtgcagcgcg
      181 cggacatcgg cgcgttcctg acgaaattcg tgctatcgaa cgtgcccgtc atcgccgtca
      241 ggctgaaccc gtccgactac gcaacggagg cgggcgccct cgaggccgcg ggcttcaaga
      301 cggtgagcgc cgacaacgcg ttctggtgga aggatggcga atgatgactc gaacgtatac
      361 gagccggccc cgggccggca tggtcgcggc ggcgatagcc gcggcgcttc tagcggcctc
      421 ctgcgccggc gcccccgcgt ccgcgcccga aacggttacg cccgcggcga cggaggcgcc
      481 cgcggtagcc tcatccaggc cccttccctc ctcgccggtc tccggatgga aactgtcggc
      541 cgagccagga aaattcatgc gggccaacct gacgaccata gagaccagaa ccctggccaa
      601 cggcgtcaag ctgatcgtca agaaaaaccc ggccaaccgc gtctattcga tgaaggtggc
      661 gttccgcggc ggctccgcga tgacgagcgc cgagaaggct ggcatagagg cgatgaccct
      721 ggccatgctg acgcgcggct cgtccaagtt cagctacgcg gcaatccagc gcctgcagta
      781 cgagctgtcg agttcgatag gctatagcgc ctcgagctac gactgggcca gcttcgacct
      841 gaatacgatc gacaagtact gggaccagat gctcgccgtc tacgccgact gcctgctcaa
      901 cccggccttc gacccggcgc aattttcgct cgttcagaac gacttcaggg taagcctcca
      961 gaaagcccag gccgacccct atagctacgc ggtcacgaag ctccacgact ccatgttcgc
     1021 cggccacccc tacgccgccg acttcggcgg gagcgccgcc tcgatctcgg cgatcagcct
     1081 cgacgacgtc aaggccttct accgcgacgc gatagcggcc gagcgcatga tcgtcgtcgc
     1141 cgtgggcaac ttcgacacgg acgccctggc cgcctcgctc aacgcaagca tcggcgccgt
     1201 accgaccaag ggctcggcca tccccgaggt ggcgcgctac gacgcgaggc ggtcgctcat
     1261 gctcgagcgc ttcgacaagt ccaagggcat aggctacgtg cggggcgact tcccgatagc
     1321 gacccccgac agccccgact tcgcgacgct gc
//
LOCUS       DAOPHV010000729         1351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_144423_length_1351_cov_4.267747, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000729 DAOPHV010000000
VERSION     DAOPHV010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144423_length_1351_cov_4.267747"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..98
                     /locus_tag="PLQ29_13790"
     tRNA            27..98
                     /locus_tag="PLQ29_13790"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Gln,seq:ttg)
     gene            135..779
                     /locus_tag="PLQ29_13795"
     CDS             135..779
                     /locus_tag="PLQ29_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPG87767.1"
                     /translation="MTQHVINAGAREVGTKGALNELRRNGGIPAIIYNRHGKTTALSL
                     DAKEFQKSLEGVSESTIVKLNVGSESFDCLVKDRQIDWLHGKILHVDFFEVEKGVAMR
                     AKVPVHLIGIPVGVRNGGILENPVHEIEVECMPKDMPEKFELDISGLEANHSIHVRDV
                     AHADAVKLLSAADQVVVLVKFAKAEAEPEAAAAPAAAAAAPAAAAAAPATPAAK"
     gene            866..>1351
                     /gene="tilS"
                     /locus_tag="PLQ29_13800"
     CDS             866..>1351
                     /gene="tilS"
                     /locus_tag="PLQ29_13800"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPG87768.1"
                     /translation="MNRVGRPGTRASAIPAAVRTFLAREPKRLGAVLVALSGGPDSVA
                     LLHATAIEAKAAAVSVTACWVDHAIRPEAELAAEREFVVGLCERLGVPLIVEALRRGA
                     VEAAAKAEGGIEAAARRFRYDALERARIASSSDLVLTGHNADDRVETLLMRFCLPRCA
                     TL"
BASE COUNT          241 a          450 c          430 g          230 t
ORIGIN      
        1 acgtcccttg cgcggcggcg taccactggg acgtcgacaa gaggtaagtc agcgggcttt
       61 ggttccgcca ttcgcaggtt cgaatcctgc cgtcccaacg cgacacatta cgatcagtac
      121 gaggagagac atccatgacc cagcatgtca ttaacgccgg agcccgcgag gtcggcacca
      181 agggcgccct gaacgagctt cgaagaaacg gcggcatccc cgcgatcatc tataaccgcc
      241 atggcaagac cacggccctt tcgctcgacg cgaaggagtt ccagaaatcg ctggaaggcg
      301 tatcggagag cacgatcgtc aagctgaacg tcggttccga gtcgttcgat tgcctcgtca
      361 aggatcgcca gatcgattgg ctccacggca agatcctgca cgtcgatttc ttcgaggtcg
      421 agaagggcgt cgctatgcgc gccaaggtgc ccgtgcacct tatcggaatc cctgtcggcg
      481 tccgcaacgg cggtatcctc gagaatccgg tccacgagat cgaggtcgag tgcatgccca
      541 aggatatgcc cgagaagttc gagctcgata tctccggcct cgaggctaac cactcgatcc
      601 acgtacgtga tgtcgcccat gccgacgccg tcaagctgct gagcgccgcc gaccaggtcg
      661 tggtgctcgt caagttcgcc aaggccgagg ctgagccgga ggccgccgcc gctccggccg
      721 ccgccgctgc cgctccggcc gccgctgccg ctgctcccgc gactcccgcc gcgaagtaat
      781 agaaccagaa cacgcattac ggggccgtcg atttcggcgg cccttatcat tgtacggcta
      841 ttgcgactcg tggaggcagt gcgtcatgaa ccgtgtcgga cggcctggaa cgcgcgcgtc
      901 ggctattccg gccgccgtgc ggaccttcct ggcccgcgag ccgaagcgcc taggagccgt
      961 tctcgtggcg ctatccggcg ggcccgactc cgtagccttg ctccatgcga cggcgatcga
     1021 ggctaaggcc gcagctgtct ccgtcacagc ttgctgggta gaccacgcca tccgccccga
     1081 ggccgagctc gccgcggagc gcgaattcgt cgtagggctg tgcgaacgcc tcggcgttcc
     1141 gctgatcgtc gaggcgctga ggcggggcgc cgtcgaagcg gccgccaagg cggagggcgg
     1201 gatagaggcg gccgcgagga ggttcaggta cgacgccctc gagcgcgcca ggatcgcttc
     1261 gtcgagcgac ctcgtcctga ccggccataa cgccgacgat cgcgtcgaga cgctcctgat
     1321 gcgcttctgc ttgcccagat gcgcgacact c
//
LOCUS       DAOPHV010000730         1350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_144612_length_1350_cov_3.580695, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000730 DAOPHV010000000
VERSION     DAOPHV010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144612_length_1350_cov_3.580695"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="PLQ29_13805"
     CDS             <1..943
                     /locus_tag="PLQ29_13805"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HPG87769.1"
                     /translation="DVEGLLIIAELESGGSSSVVEIRGGHTDVVRIERDGRPLPLPGG
                     LDTATAKPGAAAPEDTEPPYERLTLAGIYAYATEADLGPVKGALDEQIRCNSAISREG
                     LERPYGERVGATLLRCYGDGVMIMARAAAAAGADARMGGCAMPVIINSGSGNQGITAS
                     VPVIVFARDKGVSDETLHRALALSNLVSIHIKRGIGKLSAFCGAVSAACGSGAGITWL
                     SGGDLETVAMTVTNILANTSGIICDGAKASCAAKVASSLDSAILSHCMAMDRMVFKPG
                     EGIVMGDIEDTIRSVVRIGRDGMRSTDREILNIMMGC"
     gene            1007..1195
                     /locus_tag="PLQ29_13810"
     CDS             1007..1195
                     /locus_tag="PLQ29_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87770.1"
                     /translation="MAEKETLVIASKVKAFIKDASELKVSAAVIDVLSDKVRSLCEEA
                     IKNAKADGRKTVQEKDFK"
BASE COUNT          271 a          407 c          448 g          224 t
ORIGIN      
        1 ggatgtcgag gggctcctca tcatcgccga gctggaatcg ggcggctcga gctccgtcgt
       61 cgagatccgc ggtggccata ccgacgtcgt caggatcgag cgcgacggcc ggcccttgcc
      121 cctgccagga ggcttggata ccgcgactgc gaagcccggg gccgccgcgc ccgaggacac
      181 ggagccgccg tacgagcgcc tgacgctcgc cggcatctac gcgtacgcga ccgaggccga
      241 cctggggccg gtcaagggcg cgctcgacga gcagatccgc tgcaactcgg ctatatcgcg
      301 cgaaggcctc gagagaccgt acggcgagcg ggtcggggcg acgttgctac gctgttatgg
      361 cgacggggtc atgatcatgg ccagggcagc ggccgccgcc ggcgcggacg cgcgcatggg
      421 cgggtgcgcc atgcccgtca ttattaactc cggcagcggc aaccagggca tcaccgcgag
      481 cgtaccggtc atcgtcttcg cgcgcgataa gggcgtctcg gacgagaccc tgcatcgggc
      541 gctggcgctc agcaatctcg tcagcatcca catcaaacgg gggatcggga aactgagcgc
      601 cttctgcggg gcagtttcgg cggcctgcgg tagcggggcc ggcatcacct ggctgtcggg
      661 cggcgacctc gagaccgtcg ctatgaccgt caccaatatc ctggccaata cctccggcat
      721 catctgcgac ggagccaagg cgagctgcgc tgccaaggtc gcctcatcgc tggattcggc
      781 gatcctgagc cactgcatgg ctatggatag gatggttttc aagccaggcg aaggcatcgt
      841 gatgggcgac atcgaggata cgatacggag cgtggtcagg atcgggcggg atggtatgcg
      901 atcgaccgac cgagagattt tgaatattat gatgggctgc taatttccac tcgacaagcg
      961 gcgccgctat ggtctacgat aacgcattcg atcgcaagga gaacatatgg cggaaaaaga
     1021 gaccctagta atcgcctcca aggttaaggc gttcatcaag gacgcttccg aactcaaggt
     1081 ttcggcggca gttatcgacg tgctgtccga taaggtccgc tccctctgcg aagaggctat
     1141 caagaacgcc aaggcggatg gccgcaagac cgtccaggaa aaagacttca agtagagctt
     1201 cgtggcgccg ggtcgtccaa acgggacgtc ccggagctcg tcgaaaggaa gccgcgcatc
     1261 aaacacgatg aggcgattcc gcgcaaaggc accctcgccc aggatcagaa gctggccacg
     1321 gcttggttat cgaccaagtc gcgctttggc
//
LOCUS       DAOPHV010000731         1348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_144954_length_1348_cov_4.984532, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000731 DAOPHV010000000
VERSION     DAOPHV010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144954_length_1348_cov_4.984532"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PLQ29_13815"
     CDS             complement(<1..947)
                     /locus_tag="PLQ29_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG87771.1"
                     /translation="MSLIRVRSLSYTYPGAESPALSDVSLELDDGEYLAIVGANGSGK
                     STLVRCLNGLLVPPAGSVSVAGADPSDPVGRAAARRALSIVFQSPPDQLVASVVEEDV
                     AFGLENLGVPRQEMRERVAAALRDVGLESERTRSPRFLSAGQQQRLAVAGAIAMRPRA
                     VAFDEATSMIDPVGRDDVLRLMDELVAEGVAVLHVTHDMDEAARASRVLVLSDGRVAY
                     DGSPNGLFASGEAARYRLGLPRSYAAALELGLEPVVGESAAALGHRIASSDGPAEAAR
                     RLVGISRTPEARAAAATGSAATGSAATGSATTGSAATDSA"
     gene            1124..>1348
                     /locus_tag="PLQ29_13820"
     CDS             1124..>1348
                     /locus_tag="PLQ29_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87772.1"
                     /translation="MRCRLCGAEAAEFVSGGAGYGECPVCGYIGLEPSRLPSPTAEEA
                     RYRLHRNSRGDSGYVAWILEFIDAVEPYLQT"
BASE COUNT          211 a          491 c          453 g          193 t
ORIGIN      
        1 gccgagtccg tggctgccga gcccgtggtt gccgagcccg tggctgccga gcccgtggct
       61 gccgagcccg tggctgcggc ggccctggcc tccggggttc gcgatatgcc gacgaggcgc
      121 ctagcggctt cggccggacc gtccgatgag gctatcctgt gcccgagagc cgcggcgctt
      181 tcccccacga ccggctcgag ccctagctcg agcgccgccg catacgaacg cggcaatccg
      241 agccggtagc gcgccgcctc tcccgaggcg aacagcccgt tcggcgagcc atcgtaggcc
      301 acccggccgt ccgacagcac gagcacgcgg gaggcccggg ccgcctcgtc catgtcatgc
      361 gtcacatgga gcacggcgac gccctcggcg accagctcgt ccatcaggcg aagcacgtcg
      421 tcgcggccca ccgggtctat catcgaggtc gcctcgtcga aggccacagc cctcggccgc
      481 atcgcgatcg ctccggctac ggccaggcgc tgctgctggc cggccgacag gaagcggggc
      541 gagcgcgtcc gctccgactc gagcccgacg tcgcgcagcg ccgccgccac gcgttcgcgc
      601 atctcctggc ggggcacccc gaggttctcg agcccgaacg cgacatcctc ttcgacgacc
      661 gaggcgacga gctggtccgg aggcgactgg aagacgatgg acaaggccct gcgggcagcc
      721 gccctgccga ccgggtcgga aggatcggcc ccggccaccg agaccgaacc ggccggcggg
      781 acgaggaggc cgttcaggca acggacgagg gtgctcttac cggagccgtt ggccccgacg
      841 atggccagat actcgccatc gtcgagctcg aggctcacat cggacagggc cggggactcg
      901 gcccctggat aggtataact cagggatcga acacgtatca gcgacatacg cgccttcgaa
      961 ggctcgcgag cgtggcgtgg gtcatggtac gtcctcgcgt ctcggtccgt tcaccggatc
     1021 caagcgtagg ccaaagctac gcagaactcg cctccatgtc aagccgcgcc gtcgcccgga
     1081 gcggccttcc atctgcgcct ccgtccgtca tagtatccac gccatgagat gcagattatg
     1141 cggcgccgag gccgcggaat tcgtatcggg cggagccggc tacggcgagt gtccggtctg
     1201 cggctacatc ggcctcgagc cgtcccgcct gccttcgccc acggcggagg aagcccgcta
     1261 taggctgcac cgcaactctc ggggcgactc gggctacgtc gcgtggatac tcgagttcat
     1321 cgacgccgtc gagccgtacc ttcagacc
//
LOCUS       DAOPHV010000732         1348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_144963_length_1348_cov_3.800464, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000732 DAOPHV010000000
VERSION     DAOPHV010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144963_length_1348_cov_3.800464"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..351
                     /locus_tag="PLQ29_13825"
     CDS             124..351
                     /locus_tag="PLQ29_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPG87773.1"
                     /translation="MADFESVNKAIQQVRPSLQADGGDIELISVSDQGKVTVRLKGAC
                     GSCPMALMTLKQGVEAYLKKVMPDVSEVVQG"
     gene            complement(362..>1348)
                     /gene="cheB"
                     /locus_tag="PLQ29_13830"
     CDS             complement(362..>1348)
                     /gene="cheB"
                     /locus_tag="PLQ29_13830"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HPG87774.1"
                     /translation="PDVIVLDIEMPEMNGIEFLKERKRLGIEIPVVILSSVAKSGAQV
                     TMEALSLGAADFITKPSGSVSSDIHTVAGELSRMLIAYGSQYQREKGKEPPRYDFSSP
                     ERMFEAPSGEPSAAKPLARREPRPAPPKPKRAPASTEIVAIGISTGGPNALREVFARI
                     SPDLGLPVVVVQHMPAGFTEEFAASLDRICPLEVKEAADGDLLKPGRILIAPGDWHIV
                     VERRSLAGVVRINQDPPENGHRPSADVLFRSVAREYGNKALAVIMTGMGKDGAIELGS
                     VFGEGGITLGQDEASSVVYGMPKVAFEMGHVMEQVPLGSMAARISELAMEKR"
BASE COUNT          243 a          408 c          413 g          284 t
ORIGIN      
        1 tatcaccatg ctttcgatgc tatccgtggt tttgatccat aagattcgga acactatgag
       61 cgcctatgaa ccaggcttga cccctccctg cggcggtcgt tacattattg cgcaggagtt
      121 cacatggcag atttcgagag tgttaacaag gctatacaac aggtcaggcc atcgcttcag
      181 gccgacggtg gcgatataga gctcatctct gtttcggatc aaggcaaagt cacggtacgg
      241 ctcaagggag cctgtggttc ctgcccgatg gcccttatga ccttgaagca aggcgtcgag
      301 gcatacctta agaaggtgat gcccgacgtt agcgaagtgg tccagggctg agctaagccg
      361 cctatcgttt ctccatggca agctccgaga tgcgggcggc catactgccg agcggcacct
      421 gctccatgac gtgtcccatc tcgaaggcga ccttcggcat gccatagacg acgctcgacg
      481 cctcgtcctg cccgagcgtg ataccacctt ctccgaaaac cgatccgagt tcgatcgccc
      541 cgtcctttcc catgccagtc atgatcacgg cgagggcctt gttgccgtat tccctggcta
      601 cacttcggaa aagaacgtcg gcgcttggcc tgtgcccgtt ctccggagga tcctgattga
      661 tccgtacgac gcctgcgagc gagcgcctct cgacgactat atgccagtcc cccggggcta
      721 tcaggatgcg gcccggtttg agtaggtcgc cgtcggctgc ctctttaacc tcgagcgggc
      781 atatacggtc gaggctggcg gcgaattcct cggtaaatcc ggccggcata tgctgtacca
      841 cgacgacggg tagcccgaga tccggcgata ttctggcgaa gacctcgcgg agtgcgttcg
      901 ggccgccggt cgagatgcct atcgctacga tctctgtgga cgcgggggct cgcttgggct
      961 tgggcggcgc cggcctcggc tcgcgccgag cgaggggctt ggcggcggac ggctctcccg
     1021 agggggcctc gaacatgcgc tccggcgacg agaagtcgta gcgaggcggc tccttgccct
     1081 tctcgcgctg gtactgggag ccgtaggcga tgagcatcct cgatagctcg ccggcgacgg
     1141 tgtgtatatc cgaggacacc gagcccgagg gcttcgtaat gaaatcggcg gccccgaggg
     1201 acaaggcctc catcgtcacc tgagctccgc tcttggcgac gctggatagg atgacgaccg
     1261 gtatctctat gcctagccgc ttgcgctcct tcaggaattc gatgccgttc atctcgggca
     1321 tctctatgtc gagcacgatg acgtcggg
//
LOCUS       DAOPHV010000733         1344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_145670_length_1344_cov_2.421257, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000733 DAOPHV010000000
VERSION     DAOPHV010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145670_length_1344_cov_2.421257"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..913)
                     /locus_tag="PLQ29_13835"
     CDS             complement(17..913)
                     /locus_tag="PLQ29_13835"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPG87775.1"
                     /translation="MMPVSPELAREIFWIEAWHIAALVLNVGGNALLHRYADKTSLLR
                     AYQWLQGALLLWIVSKMLKTVSPRVELRWFFIVTQYLAICFIGPLFLSFAVRFRYDRA
                     PSIPLMRALYLVSAAFFGLFATNPLHGLFYSYFDFWRDRFGPVFPWFTGYTYSLIGVS
                     IAFCLMRIRKIRSMAVGLLIATAALIPLVINILYTSRVIRPLFDVTPIAMTTSIFFFA
                     LAALRFNFLGPIPVARSIVLETLDDLVVFTDGAGRVVHARGAPRGFVIGSTALSDELT
                     LGDRVYRLHSSRLKGKRDEWSR"
     gene            complement(910..>1344)
                     /locus_tag="PLQ29_13840"
     CDS             complement(910..>1344)
                     /locus_tag="PLQ29_13840"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPG87776.1"
                     /translation="DEGLDTKIALLTTFPDAEMVHRAIALGVDGFLLKDVEPAVFVRA
                     IRAIAGGLVVFQEALKPFLAAGRLQPTGAPPSDMGLTDKDLDYIRHIVNGLGNKEIAF
                     RESCSEGTVKNRVSSILAKLGLEARTQVAVFAIKRGLVVDLE"
BASE COUNT          273 a          389 c          472 g          210 t
ORIGIN      
        1 cgctgggcga ccgggtctac cggctccact cgtcgcgctt ccccttgagc cgcgacgagt
       61 ggagccggta gacccggtcg cccagcgtca gctcgtcgga tagcgctgtc gagcctatga
      121 cgaaccctcg cggggcgccg cgcgcgtgga cgactcggcc ggctccgtcg gtgaagacga
      181 cgaggtcgtc gagggtctcg agcactatcg accgagctac ggggataggg ccgaggaagt
      241 tgaagcgcag ggccgccagg gcgaagaaga agatagaggt cgtcatggcg atgggcgtca
      301 cgtcgaagag cgggcgtatg acgcggctcg tatagaggat gttgatcacg aggggaatga
      361 gggcagccgt cgctatgagc agcccgacgg ccatggagcg tatcttgcgt atcctcataa
      421 ggcagaacgc gatgctgacg cctatgagcg agtacgtata gccggtgaac cacgggaaga
      481 ccgggccgaa gcggtcacgc cagaaatcga aatagctata gaagagccca tggaggggat
      541 tggtggcgaa gagcccgaag aaggcggcgg aaacgaggta gagggcgcgc atcaggggga
      601 tcgacggggc cctgtcatag cggaagcgca cggcgaagga caggaacagc ggcccgatga
      661 aacagatggc caggtactgg gtgacgatga agaaccagcg cagctccacg cggggagaga
      721 cggtcttgag catcttactg acgatccaca ggagcagggc tccctggagc cattgatacg
      781 ccctgagcag ggacgtcttg tcggcgtagc gatgcaggag agcgttgccg ccgacgttca
      841 ggaccagcgc ggctatatgc cacgcctcga tccagaatat ctcgcgggcg agctcgggcg
      901 agaccggcat cattccaggt cgacgacgag gccgcgcttg atggcgaaca ccgctacctg
      961 ggtacgcgcc tcgagcccga gcttcgcgag tatggacgat acgcggttct tgaccgttcc
     1021 ctccgagcac gactcccgga aggctatctc cttattgccg aggccgttga cgatatggcg
     1081 tatgtagtcc aggtccttat cggtaaggcc catgtccgac ggcggggcgc cggtcggctg
     1141 cagcctcccg gcggctagga acggcttgag cgcttcctgg aagacgacga ggccgccggc
     1201 tatcgccctg atggcccgta cgaagaccgc cggctcgacg tccttgagca ggaatccgtc
     1261 gaccccgagc gcgatcgccc gatggaccat ctccgcgtcc gggaaggtgg tcagcagcgc
     1321 tatcttggtg tcgaggccct cgtc
//
LOCUS       DAOPHV010000734         1342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_145966_length_1342_cov_2.816628, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000734 DAOPHV010000000
VERSION     DAOPHV010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145966_length_1342_cov_2.816628"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..105)
                     /locus_tag="PLQ29_13845"
     tRNA            complement(25..105)
                     /locus_tag="PLQ29_13845"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69..71),aa:Leu,seq:tag)
     gene            complement(191..263)
                     /locus_tag="PLQ29_13850"
     tRNA            complement(191..263)
                     /locus_tag="PLQ29_13850"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(228..230),aa:Lys,seq:ctt)
     gene            complement(290..363)
                     /locus_tag="PLQ29_13855"
     tRNA            complement(290..363)
                     /locus_tag="PLQ29_13855"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(327..329),aa:Arg,seq:tcg)
     gene            complement(406..477)
                     /locus_tag="PLQ29_13860"
     tRNA            complement(406..477)
                     /locus_tag="PLQ29_13860"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(443..445),aa:His,seq:gtg)
     gene            complement(522..>1342)
                     /locus_tag="PLQ29_13865"
     CDS             complement(522..>1342)
                     /locus_tag="PLQ29_13865"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPG87777.1"
                     /translation="YHFMPGSATYSVGYLGCNLRCPFCQNHGISQRTDAPTWRVEPAG
                     LVAEARAARCPSLAHTYSEPLAHAEFVMDCMKAAREAGLANVLVTNGYCGEEAAAAVL
                     SLCDAVNVDVKAWDEGFYREELGGDLNSVKRFVMMAVECGVHVEATTLVIPGKNDDPA
                     QIDAIAGFLAALSPDIPYHLSAYRPMYRYTIPATKASTLRSLADLARRRLRYVYSGNL
                     PGERSLTLCRACGAVLVSRMGYAVDASGLRGCRCASCGEPSPIHGARKKNQKCP"
BASE COUNT          267 a          411 c          436 g          228 t
ORIGIN      
        1 cgacgctcgg tacgtccgat ctactgcgag agaagggact tgaaccctta cgccggaggc
       61 accagatcct aagtctggcg tgtctgccaa ttccaccact ctcgctcgga actatagggt
      121 ccgaacgata tcggaatcga caaaccctgt caagggcgct cgcctcggcg acgagcgcct
      181 gagacgttct tgagccgtgc agggatcgaa cctgcgaccc gcagattaag agtctgctgc
      241 tctaccagct gagctaacgg ccctggtaca aaacacaaaa caagaacgct gcgtccggag
      301 ggagtcgaac ccccgaccta cggattcgaa gtccgtcgct ctatccagct gagctacgaa
      361 cgcccgtcgc cgatcggccc caggcgccgg catcagacga gctagggtgg atgacggggt
      421 tcgaacccgc gacaaccaga tccacattct gggactctac cgctgagcta catccaccgt
      481 gcgcttggcg agacgtacta tgccaaaacg caaaaaaagt gtcaagggca tttctggttt
      541 ttttttctag cgccgtgaat cgggctcggc tcgccgcagg aggcgcagcg gcagccccgt
      601 agtccggagg cgtcgacggc gtagcccatt ctcgagacga ggacggcgcc gcaagcccgg
      661 catagcgtga gggatcgctc gcccggtagg ttgcccgagt agacgtagcg caggcgacgg
      721 cgggccaggt cagcgaggga gcgtagcgtc gaggccttcg tcgccggaat cgtatagcgg
      781 tacatgggac gatacgccga caggtggtac ggtatgtcgg gcgacaaggc ggctaggaag
      841 ccggctatgg cgtcgatctg ggccgggtcg tcgttcttgc cgggaatgac cagcgtggtc
      901 gcctcgacgt ggacgccgca ctcgacagcc atcatcacga agcgcttgac cgagttcagg
      961 tcgccgccta gctcttcgcg atagaagccc tcgtcccagg ccttcacatc gacgttgacg
     1021 gcatcgcaca gcgacaagac cgctgccgcg gcctcctcgc cgcagtagcc gttggtgacg
     1081 agtacgttgg caaggccggc ctccctggcg gctttcatgc agtccatgac gaattccgca
     1141 tgcgcgagcg gctccgaata ggtatgagcc agcgatgggc aacgcgccgc cctcgcctcg
     1201 gcgacgaggc cggccggctc gacgcgccag gtcggggcat cggtgcgttg cgagatgccg
     1261 tggttctggc agaaggggca gcggaggttg cagcctaggt agccgacgga gtaggttgcg
     1321 ctacccggca tgaagtggta ca
//
LOCUS       DAOPHV010000735         1338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_146711_length_1338_cov_2.445051, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000735 DAOPHV010000000
VERSION     DAOPHV010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146711_length_1338_cov_2.445051"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..>1338
                     /locus_tag="PLQ29_13870"
     CDS             253..>1338
                     /locus_tag="PLQ29_13870"
                     /inference="COORDINATES: protein motif:HMM:NF018938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HPG87778.1"
                     /translation="MKKIRIGSGAGYAGDRLEPSLELIEHGALDYICYECLAERTIAI
                     AQQARLKDPSKGYNALLEYRMNAALPLAHKHGVKIITNMGAANPLAAVEVVKRIARDK
                     GISGIKIAAVYGDDVFPRIDEYRGAAVWETGEPLERLAGEIVSANAYLGCEGIVEALK
                     RGADVVITGRVSDPALFIAPLVYEFGWKPDDWDRLGKGTVIGHLLECGGQATGGYFAD
                     PGKKDVPEPWRLGFPLIEADENGDAFITKVVGSGGVVTTDTCKEQLIYEIHDPSTYIT
                     PDVTADFSRVSFTQSGKDVVEIRGATGRARTDTLKVSIGYKDCFIGEGEISYGGPNCV
                     ERARLAREIVRRRLELRAVPTDELRMDI"
BASE COUNT          249 a          448 c          430 g          211 t
ORIGIN      
        1 ttcccggtga gccccctgac cggctccacg ttcctgctca tcggcctcgc cggcgtcgac
       61 ctcggcgagc accagaagaa gaccattccg ttggcgttcg ccacgaccct ggtcatgctg
      121 ctcgtcgccg tagtcatagg cgtcattcct ctctagcgtc cgtcgctact gccgccgggc
      181 ggagcaatcg cctccgctcc gtccggcgtt tcctttcttt agggcaggcc ccacggggcc
      241 gggagattca ccatgaagaa gatccgaatc gggagcggcg ccggctacgc cggcgacagg
      301 ctcgaaccgt cgctcgagct gatcgagcac ggcgcgctcg actacatctg ctacgagtgc
      361 ctcgccgagc gcaccatagc catagcccag caggccaggc tcaaggaccc gtcgaagggc
      421 tacaacgccc tgctcgagta ccgcatgaac gccgcccttc cgctggcgca taagcacggc
      481 gtcaagatca ttaccaacat gggcgccgcc aatcccctgg cggcggtcga ggtggtcaag
      541 cgaatagcgc gggacaaggg catctcgggc atcaagatag ccgccgtcta cggcgacgac
      601 gtgtttccca ggatcgacga gtaccgcggc gccgccgtct gggagaccgg cgagccgctc
      661 gagcggctcg caggcgagat cgtatcggcc aacgcttacc taggatgcga gggcatcgtc
      721 gaggcgctca agcggggcgc ggacgtggtg ataacaggcc gcgtttccga ccccgccctg
      781 ttcatcgcgc cgctcgtcta tgaattcggc tggaagcccg acgactggga taggctcggc
      841 aagggtaccg tcatcggcca cctgctcgaa tgcggcggcc aagccaccgg cggctatttc
      901 gccgaccccg gcaagaagga cgtaccggag ccctggcgcc tcggcttccc gctcatcgag
      961 gcggacgaga acggcgacgc gttcattacc aaggtagtcg gctcgggcgg cgtcgtcact
     1021 acggacacct gcaaggaaca gctgatctac gagatacacg acccgtctac gtatatcacg
     1081 cccgacgtca cggccgactt ctcgagggta tcgttcaccc agtccggcaa ggacgtcgtg
     1141 gagatacggg gcgccacggg cagggccagg accgacacgc tcaaggtaag catcggctac
     1201 aaggactgct tcatcggcga gggggagatc agctacggcg gccccaactg cgtcgagcgg
     1261 gccaggctgg cgcgagagat cgtgcgcagg cggctcgagt tgcgcgccgt gccgactgac
     1321 gagctgcgca tggacata
//
LOCUS       DAOPHV010000736         1331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_147834_length_1331_cov_4.907524, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000736 DAOPHV010000000
VERSION     DAOPHV010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147834_length_1331_cov_4.907524"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PLQ29_13875"
     CDS             <1..678
                     /locus_tag="PLQ29_13875"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPG87779.1"
                     /translation="AFIAISVLDAAGVNLSALLGAAGIAGIAIGFAAQTSVSNFISGF
                     FIVSEKTFSQGDVIDVDGTSGLVHSIDAMSVKLRTFDNRLIRIPNETLIKSNVKTITR
                     FPIRRLNMDILVTYDTDIERARQVLLELAAAEPNALRSPEPVFMVSGFRDSGIALFFG
                     VWFATNEWFDGNNAMHIAVKKRFDEEGIRFALPTRTVYQGKAVAAPKKPGARRAAAGG
                     PRGNNGS"
     gene            790..1236
                     /locus_tag="PLQ29_13880"
     CDS             790..1236
                     /locus_tag="PLQ29_13880"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPG87780.1"
                     /translation="MSHESALSKVSLFANLEPKYIKGIAQISTERDFAPGDHLMKQGE
                     DGIGLFIILSGKVKIEKVNANGQSVEIASNGPGDVMGELAVLDGAKRTATVTAVEPTK
                     CLVLASWEFNSFMKSHPEVGTAILPIVVRRFRETNDALVGLSGVKA"
BASE COUNT          262 a          432 c          410 g          227 t
ORIGIN      
        1 gcattcatcg cgatcagcgt gcttgacgcg gccggcgtca acctatcggc cctactcggc
       61 gccgccggca tagccggtat cgccatcggc ttcgccgccc agacctccgt gtccaacttc
      121 atctcgggct tcttcatcgt ctcggagaag accttctccc agggcgacgt gatagacgtg
      181 gacggcacct ccggtctcgt ccactcgatc gacgcgatgt cggtcaagct acgcaccttc
      241 gacaaccgcc tcatacgcat accgaacgag acgctgatca agtcgaacgt gaaaaccatc
      301 acgcgcttcc cgatacggcg gctgaacatg gacatcctcg tgacctacga caccgatatc
      361 gagcgcgcca ggcaggtgct cctcgagctg gccgccgccg agccgaacgc ccttcgcagc
      421 ccggaaccgg tcttcatggt gtcgggcttc agggacagcg gcatagccct gttcttcggc
      481 gtctggttcg cgacgaatga gtggttcgac ggcaataacg cgatgcacat agccgtcaag
      541 aagcgcttcg acgaggaggg catacgcttc gcgctgccga cgcggaccgt gtaccagggg
      601 aaggccgtag ccgcgcccaa gaagcctgga gcccggcgcg ccgcggccgg aggcccgcgc
      661 ggaaacaacg gttcatagtc gaaccgtagc cccgttaacc ggagacttca ggttgacggt
      721 ccgccgacct aggcgtatct tgagataagc cgcgcaccgt agcgtagcgg ccttatacgg
      781 aggtactgga tgagtcatga aagcgcgctc tcgaaggtca gcctgttcgc caacctcgag
      841 ccgaagtata tcaagggcat agcgcagatc tcgaccgagc gcgacttcgc ccccggcgat
      901 cacctgatga agcagggcga ggatggcatc ggcctgttca tcatcctgtc gggcaaggtc
      961 aagatcgaaa aggtgaacgc caacggccag tcggtcgaga tagcctcgaa cggccccggc
     1021 gacgtgatgg gcgagctggc cgtgctcgac ggcgccaagc gcaccgccac ggttaccgcg
     1081 gtcgagccga ccaagtgcct cgtgttggcc tcgtgggagt tcaactcctt catgaagagc
     1141 caccccgagg tcggcacggc catcctgccc atagtcgtgc gacgcttccg cgagacgaac
     1201 gacgccctcg tagggctgtc aggcgtcaag gcctaagcgc gcggggccga cgatcggcca
     1261 ggtacggaac gggcggcttc atgacggaag ccgcctgttt tttatcatcc cgggtggtac
     1321 gaaccttgtg c
//
LOCUS       DAOPHV010000737         1325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_148897_length_1325_cov_2.985039, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000737 DAOPHV010000000
VERSION     DAOPHV010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148897_length_1325_cov_2.985039"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PLQ29_13885"
     CDS             complement(<1..536)
                     /locus_tag="PLQ29_13885"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPG87781.1"
                     /translation="MKHVLVVLAALSILAGPLSAQAERPLRVAVAEFQTKGEATFEDA
                     GRIIPEWLIGSLSRIPEFDVVERVLLDRVLEEQGLALSGLVGEGGTTAGALAGADAVV
                     AGSVIAWAGSLSVTVRLIDATSGEVIRAVTLQDASPKSLPSELDGVARVLAGQQPASA
                     LRPPAVPGVKAKEYPVSV"
     gene            complement(602..>1325)
                     /locus_tag="PLQ29_13890"
     CDS             complement(602..>1325)
                     /locus_tag="PLQ29_13890"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPG87782.1"
                     /translation="MQLVRTDHQIDFPERLRQDIDSFLTLLEYEREELLGRRNLGAKV
                     RKLVDTIDYWGYLVSENQHNDKAAKWKFLNVESLIKGIETWEKDPDNLDPSLFAWLNR
                     ISLITRDDQEDDDSGKVNLMTIHAAKGLEFDVVFVAGCEDGLIPHARSLEEGEGNVEE
                     ERRLFYVAITRARKRLVLSACKRRRRNSMPVDCVPSPFLAEIPEELLCWREDEAPLTE
                     DEQADAWARVHRMFAQPGDEAS"
BASE COUNT          220 a          453 c          415 g          237 t
ORIGIN      
        1 atgaccgata ccgggtactc cttggccttg actcccggga cggccggggg cctgagcgcc
       61 gacgcgggct gctggccggc caggaccctg gcgaccccgt cgagctccga aggcaggctc
      121 ttcgggctcg cgtcctggag cgtcacggcg cgtatcacct cgccgctcgt cgcgtcgatc
      181 agccggacgg tcaccgacag gctaccggcc cacgctatga ccgagcccgc caccacggcg
      241 tccgcccccg ccagggcccc ggccgtggtc ccgccctcgc cgacgaggcc ggatagagcc
      301 aggccctgct cctcgagcac gcggtcgagg agcacccgct ccacgacgtc gaactcgggg
      361 atcctcgata gcgagcctat gagccattcg gggataatgc ggccggcgtc ctcgaacgtg
      421 gcctcgccct tcgtctggaa ctcggctacc gccacgcgca acggacgctc ggcttgggcc
      481 gaaagcggcc ccgccagtat cgacagcgcc gccagtacta ccaggacgtg tttcatacga
      541 cctccggtac cagcgctcag tatagcacga gttggttgat actaaaaaaa tattatataa
      601 cctagctagc ctcgtcgccg ggctgcgcga acatcctatg caccctggcc caggcgtcgg
      661 cctgttcgtc ctcggtcagc ggcgcctcgt cctcgcgcca acacaggagt tcctcgggta
      721 tctcggccag gaagggcgac ggaacgcaat cgacgggcat ggagttgcga cggcgccgct
      781 tgcaggccga cagcacgagc cgcttccgcg cacgcgttat cgcgacgtag aagagccggc
      841 gttcctcctc gacgttgccc tcgccctcct cgagcgatcg ggcgtgcggg atgagcccat
      901 cctcgcaacc ggccacgaag accacgtcga actcgaggcc cttggccgcg tggatggtca
      961 tcaggttgac cttacccgaa tcgtcgtcct cctggtcgtc gcgggtgatt agcgagatgc
     1021 gattcagcca cgcgaacagg ctcgggtcca ggttgtcggg gtccttctcc caggtctcga
     1081 tacccttgat caggctctcg acgttcagga acttccactt ggcggctttg tcgttatgct
     1141 ggttttcgga gacgaggtag ccccagtagt cgatcgtgtc gacgagcttg cggaccttgg
     1201 cgcctaagtt acggcggccc aggagctcct cgcgctcgta ctcgagcagg gtcaggaagg
     1261 agtcgatgtc ctgcctgagc cgctcgggga agtctatctg atgatcggtg cgcacgagct
     1321 gcatc
//
LOCUS       DAOPHV010000738         1322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_149488_length_1322_cov_2.484609, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000738 DAOPHV010000000
VERSION     DAOPHV010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149488_length_1322_cov_2.484609"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PLQ29_13895"
     CDS             <1..839
                     /locus_tag="PLQ29_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HPG87783.1"
                     /translation="GLGPGASHDDYRSAGDALCRLVNEELSSMAEREYGLASRLELRF
                     DKAYARFVIPPLRVAEADGDDAAARGLAHGTTRGLAHGTTRGLAHGTTRGRGKGYAGL
                     LRGRDGDRVDVKGMEAVRSDWTPLAREFQLQLLELAFSGSGERALGDYIASTLAALER
                     GELDARLVYTRVLRRSASAYVKNTPPQVKAARLAGVTGRGAVSYVIAADGPAPVDPER
                     YGAAGPGEPRADYRHYAGKQLLPIARSLAASGLAVPLGAFGEGVPGGAGVPGVRQPEL
                     GF"
     gene            complement(836..>1322)
                     /locus_tag="PLQ29_13900"
     CDS             complement(836..>1322)
                     /locus_tag="PLQ29_13900"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPG87784.1"
                     /translation="NAFRVVPSGGCRHPLDLIIYARRVEGLAPGLYRHLATDRALALL
                     RPASLVPGADPERNGCLSLDEELDAALSGQLWNSAAVFVWTAVPYRTEWRYTVAAAKT
                     LLLDAGHACQALHGACEALGLGTCAQAAYDQQRLDEALGVDGIDEAAVYVASTGRVAA
                     R"
BASE COUNT          171 a          477 c          509 g          165 t
ORIGIN      
        1 cgggcctcgg gcctggcgcg agccacgacg actaccggag cgccggcgac gcgctctgcc
       61 gcctcgtcaa cgaggagcta tcctcgatgg ccgagcggga gtacgggctc gcttcgaggc
      121 tcgagctgcg cttcgacaag gcctacgcgc gcttcgtgat cccgccgctg cgcgtcgcgg
      181 aggccgacgg cgacgacgcg gccgcccgag ggctcgccca cgggactacc cgaggtctcg
      241 cccacgggac tacccgaggt ctcgcccacg ggactacccg tgggcgggga aagggctacg
      301 ccgggctgct gcgcggccgc gacggcgatc gggtcgacgt caagggcatg gaggcggtgc
      361 gctccgactg gacgccgctc gctcgcgagt tccagctcca gctgctcgag ctggccttct
      421 cgggctccgg cgagagagcg ctcggcgact atatcgcctc gacgctggcc gccctggaac
      481 gcggcgagct cgacgcccgg ctcgtctaca cgcgcgtcct taggcgctcg gcgtccgcct
      541 acgtcaagaa tacgccgccg caggtcaagg ccgcgcggct cgcgggcgtc accggccgag
      601 gcgccgtgtc gtacgtgatc gcggctgacg gccccgcgcc ggtcgacccg gaacgctatg
      661 gcgcggcggg tccgggggaa ccccgggccg actaccgcca ctacgcgggc aagcagctcc
      721 tgccgatagc ccgctcgctc gcggcctcgg gcctggccgt gccgctcggc gcgttcggcg
      781 agggagtccc gggaggggcg ggcgtaccgg gcgtcaggca gccggagctg ggattctagc
      841 gcgcggccac gcgccccgtc gacgcgacgt acacggccgc ctcgtcgatg ccgtcgacgc
      901 cgagcgcctc gtcgagccgc tgctggtcat aggcggcttg ggcgcaggtg ccgaggccca
      961 gggcctcgca ggcgccgtgc agggcctggc aggcgtggcc cgcgtcgagg agcagggtct
     1021 tggccgccgc taccgtgtag cgccactcgg tgcggtacgg cacggccgtc cagacgaaca
     1081 cggcggcgct gttccagagc tggcccgaca aggccgcgtc cagctcctcg tcgagcgaca
     1141 ggcagccgtt ccgctccggg tcggcgccgg gcacgagcga ggccggccgg aggagcgcca
     1201 gggctcggtc ggtcgccagg tggcggtaca ggccgggcgc gaggccctcg acgcggcgcg
     1261 cgtagatgat caggtcgagg ggatgcctgc agccgccgga cggcacgacg cggaaggcgt
     1321 tg
//
LOCUS       DAOPHV010000739         1318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_150116_length_1318_cov_3.342043, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000739 DAOPHV010000000
VERSION     DAOPHV010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150116_length_1318_cov_3.342043"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PLQ29_13905"
     CDS             <1..550
                     /locus_tag="PLQ29_13905"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPG87785.1"
                     /translation="LIADVAGHDVGTSYVTPAVKVLLKQSATPAHSVEETLAYLNGVL
                     AKTVLEDNYLTAFALRVNRRAGKVVFAAAGHPPALFIPKDGSPRLAERENPFIGMLDD
                     SSYEATSLDVAEGDRFILYTDGLIEGGGDDKASWTDAKGGLVTVAERLRGLPLEELPL
                     AFVKAMGADKADDDVAVLAVEI"
     gene            561..1013
                     /locus_tag="PLQ29_13910"
     CDS             561..1013
                     /locus_tag="PLQ29_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HPG87786.1"
                     /translation="MRLWTLDPRYLDAKGLVAAWREALLAQAVLEGRTVGYARHPQLE
                     RFRRAPDPAASLGRFLEALAGEAGARGYTFDASKIHGLDRPDPAPLPVTLGQVAYELE
                     LLRSKLNARDPELATGLPASVPVALNPVFVSTPGPVESWERVRPDVAR"
     gene            1079..>1318
                     /locus_tag="PLQ29_13915"
     CDS             1079..>1318
                     /locus_tag="PLQ29_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87787.1"
                     /translation="MDTQTLTLAVAMAGLSIVAVTALFVAFRHERSLAIFSLGFLSAM
                     VGFVLLIGQNKPHDWASFILANALIVFYQLCMAWGV"
BASE COUNT          208 a          461 c          422 g          227 t
ORIGIN      
        1 cctgatagcg gatgtggccg gccatgacgt cggtacgagc tacgtgacgc cggccgtcaa
       61 ggtgctcctg aagcagagcg cgacgccggc ccactcggtc gaggagacgc tggcctatct
      121 aaacggcgtg ctggccaaga ccgtgctcga ggacaattac ctgacggctt tcgcactacg
      181 cgtcaaccgg cgcgcgggca aggtcgtctt cgccgccgcg ggccatccgc cggccctatt
      241 catccccaag gacggctcgc cacggctggc cgagcgggag aaccccttta tcggcatgct
      301 ggacgactcc agctacgagg cgacgtcgct cgacgtcgcg gagggcgatc gcttcatctt
      361 gtataccgac ggcctgatcg agggcggcgg agacgacaag gctagctgga ccgacgccaa
      421 gggcggcctc gtaacggtgg cggaacgcct tcgcggcttg cccctggagg agttgccgct
      481 cgccttcgtc aaggccatgg gagccgacaa ggcggacgac gacgtggccg tgctggcggt
      541 agagatctag gcgccgcgcg atgcgcctgt ggaccctgga cccgcgctac ctcgacgcca
      601 agggcctcgt cgccgcctgg cgcgaggcgc tactcgccca ggccgtgctc gagggtcgta
      661 ccgtcggcta cgcccgccat ccgcaactcg agcgcttcag gcgggcgccc gaccccgcgg
      721 cgtcgctcgg ccgcttcctc gaggcgctcg ccggcgaggc cggtgctcgc ggctacacgt
      781 tcgatgcctc caagatccac ggcctcgatc gccctgaccc ggcgccgctt cccgtgaccc
      841 tcgggcaggt cgcctacgag ctcgaactac tcaggagcaa gcttaacgcc cgcgacccgg
      901 agctggctac ggggctaccc gcctcggtac cggtggcgct caacccggtc ttcgtatcga
      961 cgccggggcc cgtcgagtcc tgggagcgag tccggcccga cgtggcgcgt taaggccggc
     1021 gcggtacgcg agagccgacg gctggattcc gaatcgctaa taaactacta tgatcgccat
     1081 ggatactcaa acgttgacgc tcgccgtcgc catggccgga ctatccatcg tagccgtcac
     1141 ggcgttattc gtagcctttc gtcacgagcg tagcctcgcc atcttctccc tcggctttct
     1201 ctcggccatg gtaggcttcg tactgctgat cggccagaat aagccccatg actgggcctc
     1261 gttcatactc gccaacgctc tgatcgtctt ctaccagctc tgcatggcct ggggcgtc
//
LOCUS       DAOPHV010000740         1318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_150128_length_1318_cov_3.088678, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000740 DAOPHV010000000
VERSION     DAOPHV010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150128_length_1318_cov_3.088678"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PLQ29_13920"
     CDS             <1..638
                     /locus_tag="PLQ29_13920"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPG87788.1"
                     /translation="GFGLTRETADKLFAAGAVAITSGNHVCEKKGAAELLDGDPRILR
                     PANYPALAPGRGLCELEKGGRRWAVINLQGREGMSPIDNPFDRADELLAAIEPGSLVV
                     VDFHAESFAEKEALGLYLDGRVSVFAGTHTHVQTADERVLPKGSGYIGDLGMSGPDDS
                     IIGVRVDVCIRRNLSQMPLKMESADGPSRLRGAVFELGDDGRCLSIRRLVV"
     gene            complement(692..>1318)
                     /locus_tag="PLQ29_13925"
     CDS             complement(692..>1318)
                     /locus_tag="PLQ29_13925"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPG87789.1"
                     /translation="AHVALLAGLGYEALGVDPSEAMLAEAARRHGYLSATGRASFRSG
                     GMLDVDALVEPGGAALVLCVGNTLPHLARSELSSFLGKAARALEPGGRLVVQLLNYGR
                     LLAERPERLPDIRAGGWLFRRSYRYRADGLVEFDTELSRLDGVGVPSGDPSGADRGST
                     TLTPFTVDELLEAASEAGLEARGAYSSWARQPFEPSSSMVALLELVRT"
BASE COUNT          214 a          462 c          455 g          187 t
ORIGIN      
        1 gcggcttcgg gctgaccagg gagacagccg ataagctgtt cgccgccggc gccgtcgcta
       61 tcacgagcgg caaccatgtc tgtgagaaga agggcgccgc ggagctgctc gacggcgacc
      121 cgcgcatact caggccggcc aactatccgg ccctggcacc cggccgcgga ctctgcgagc
      181 tcgagaaggg cggtcggcgc tgggcagtca tcaacctgca gggccgcgag ggcatgagcc
      241 cgatcgacaa ccccttcgac cgggccgacg agttgctcgc ggctatcgag cccggcagcc
      301 tcgtcgtcgt cgattttcac gccgagtctt tcgccgagaa ggaggccctg ggcctctacc
      361 tggacggccg cgtatccgtc ttcgccggta cccataccca cgtccagacc gccgacgagc
      421 gcgtcctccc gaagggctca ggctacatcg gggacctcgg catgtctggc cccgatgact
      481 cgatcatagg cgtccgcgtc gacgtctgca tacgccgcaa cctgagtcag atgccgctca
      541 agatggaatc ggccgacggc ccgtcccgcc tgcgcggcgc cgtcttcgag cttggcgacg
      601 acggccgctg cctgtcgatc cgcaggctcg tcgtctgagt acagccgtcg tctgagtaca
      661 gccgtcgtct gagtacagcc gtcgtcgtgg cttaggtccg taccagctcg agcagggcga
      721 ccatcgacga ggacggctcg aagggctggc gcgcccagga cgagtaggcg cccctcgctt
      781 ccaggcccgc ttcggaagcc gcctcgagta gctcgtcgac ggtgaacggc gtcagggtcg
      841 tcgaaccgcg gtcggctccc gatgggtcgc ccgacggtac gccgacgccg tctagtcggg
      901 agagctccgt gtcgaactcg acgaggccgt cggcgcggta gcggtacgaa cggcggaaca
      961 gccagccgcc agcccggata tcgggtagcc gctcgggccg ctcggcgagg agcctgccat
     1021 agttgagcaa ctggacgacg agccggccgc ccggctccag cgcgcgggcg gctttgccca
     1081 ggaaggacga aagctcggat cgggccaggt gcggcagggt gttaccgacg cacagcacga
     1141 gggccgcccc gcccggctcg acgagagcgt cgacgtccag catgccgccc gaccggaatg
     1201 acgcccgacc ggtagccgag aggtagccgt gacgtcgcgc cgcctcggcg agcatcgcct
     1261 ccgacggatc gacgccgagc gcctcgtacc cgaggccggc cagtagcgcg acgtgggc
//
LOCUS       DAOPHV010000741         1317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_150336_length_1317_cov_2.721078, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000741 DAOPHV010000000
VERSION     DAOPHV010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150336_length_1317_cov_2.721078"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PLQ29_13930"
     CDS             complement(<1..786)
                     /locus_tag="PLQ29_13930"
                     /inference="COORDINATES: protein motif:HMM:NF020983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2012 domain-containing protein"
                     /protein_id="HPG87790.1"
                     /translation="MKKIVRIGAIALIAFAAIGVMSCDFVSFIIASVSGSAVNALAEG
                     DAVEFYKGEGTATLVGATITLTNQSTTGAHASGTVASDGTFTLYNVEPGRYILSGRHT
                     GWTFVPRLVDITGFMTTLPTVIAYPDVGEEEILLLVEWENMDYDVDSYMVRDDDASFT
                     DSTDATPGTKVAGYKTSNPYGYEVDPANKVVLERDVKVADPDSIPRVETIRVYGPVAT
                     GTTEYLRYYLRLFNQSSAYLTGLDSIEAAGATVFVMQGGTHLGT"
     gene            992..>1317
                     /locus_tag="PLQ29_13935"
     CDS             992..>1317
                     /locus_tag="PLQ29_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87791.1"
                     /translation="MKRIVLVSLALLALAAAAFGQQTVTQAAVGAASTVASADMGPGI
                     ESLKANAQEDYAVVSGDTLKAVSEAAYGDYRYWPIVYLTNKEAISNPELIEPGLAIKI
                     YRLPFDA"
BASE COUNT          292 a          372 c          365 g          288 t
ORIGIN      
        1 ggttccaaga tgggttccgc cttgcatcac gaaaacagtc gcaccagcgg cttcaataga
       61 atcgagtcca gtgagatacg ctgacgactg attaaaaagc cgcaggtagt acctgaggta
      121 ctcggtcgtt ccagtagcta ctggcccata gactcttatc gtttcaaccc tgggaataga
      181 atccgggtca gcgactttga catcccgttc taatactact ttatttgctg gatcaacttc
      241 gtatccgtac ggattagaag ttttataacc agcgacttta gtgcctggtg tagcatcagt
      301 actatctgta aacgatgcat catcatcgcg gaccatgtaa ctgtctacgt cgtagtccat
      361 gttctcccat tcaaccaata gaagaatctc ttcttctccg acatctggat acgcgataac
      421 agtggggagc gtcgtcataa agccggtgat atcaacgagc ctcggcacga aagtccagcc
      481 cgtatgcctt ccagacagga tatagcgccc cggctccacg ttgtacaatg taaaggttcc
      541 atcgctggcg acggttccgg aggcatgagc gccagtagtt gactggttgg taagcgtaat
      601 ggtagcgcca acgagtgtag cggtgccctc tcccttgtag aattctacgg cgtctccttc
      661 cgcgagcgcg ttcacggcgc ttccgctgac gctagcgatt atgaagctga cgaaatcgca
      721 gctcatcacg ccgatggccg cgaacgcgat gagcgcgatc gctccgatgc gtactatctt
      781 cttcattcga tcctccttat ggactacgaa catactcaca ggcgctccgt ttaacgcgcg
      841 ggcgactgaa aggcatctgt aagtatatga cctttccggc gaaaatcaac aagcccccga
      901 tcgggcgaga aaatttgcat tcgccgagcg ccggcgctag gcttgagtcc gcgcccgtag
      961 ggccgagatg taatggtatg aggagggact catgaaacga atcgttctgg tatcgttagc
     1021 gttgctggcg ttggccgcgg cggcgttcgg acagcagacc gtgacccagg ccgcggtcgg
     1081 ggccgcgtcg acggtggcgt cggccgacat ggggccgggc atcgagagcc tcaaggccaa
     1141 cgcgcaggag gactacgcgg tggtctccgg cgacaccctc aaggcggtgt ccgaggccgc
     1201 gtacggcgac tatcgctact ggcccatcgt ctacctgacc aacaaggagg cgatctccaa
     1261 tcccgagctg atcgagccgg gcctggctat caagatctac aggctgccct tcgacgc
//
LOCUS       DAOPHV010000742         1313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_151064_length_1313_cov_3.558824, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000742 DAOPHV010000000
VERSION     DAOPHV010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151064_length_1313_cov_3.558824"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..89)
                     /locus_tag="PLQ29_13940"
     CDS             complement(<1..89)
                     /locus_tag="PLQ29_13940"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HPG87792.1"
                     /translation="MRIYMLEKLRERPWCVCELAAIVGVDKSVA"
     gene            259..510
                     /locus_tag="PLQ29_13945"
     CDS             259..510
                     /locus_tag="PLQ29_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPG87793.1"
                     /translation="MKVQILGSGCPKCKLLEQHAREAVAELGVDAEIVKVTNTDDIME
                     MGVMMTPALAIDGEVKSVGKVLTKDQVASYLKVVKFHAL"
     gene            500..868
                     /locus_tag="PLQ29_13950"
     CDS             500..868
                     /locus_tag="PLQ29_13950"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HPG87794.1"
                     /translation="MPCKCDGTCETTLIYACSGAANTGLLADQVMRILSRNKVGSSTC
                     LAAMGADLSGFLESARNATRNIVLDGCKVSCGKRIFEKNGLAYQHYVMTDYDVEKGKT
                     PITGDLTEAVAERIEGLVKA"
     gene            865..>1313
                     /locus_tag="PLQ29_13955"
     CDS             865..>1313
                     /locus_tag="PLQ29_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPG87795.1"
                     /translation="MSSKEISPNQKLLIVFGVFLAAYFIPWGAARVLGATNEAFMMLG
                     EYARQHVLLCLVPAMFIAGAITVFLNQQAVMRYLGPDANRFTAYGVASVSGAILAVCS
                     CTVLPIFKGIYKKGAGLGPAVAFLYSGPAINILAIVLSAKVFGWKLGV"
BASE COUNT          273 a          376 c          397 g          267 t
ORIGIN      
        1 gccactgact tgtcgacacc aacgatggcg gccagctcgc agacgcacca ggggcgctcc
       61 cgcaacttct cgagcatgta tatcctcatc gggtgggcca gagccttgaa catctccgtc
      121 ctcagttcat agcgtcgcaa tgctatatcg tccatagact gaatgttgca atgtttctaa
      181 acaattgtca agaaggtact tgacaattgt gtgcattgtt tcaataattt gcaacatgag
      241 gttgcaatgg aggattctat gaaggtacag attcttggtt ccggttgtcc caaatgcaag
      301 ctactggaac agcacgcccg cgaggcggta gccgagctcg gcgtagacgc ggagatcgtc
      361 aaggtgacga ataccgacga catcatggag atgggcgtga tgatgacgcc cgcgctggcg
      421 atagacggcg aggtcaagag cgtcggcaag gtgctgacga aggaccaagt cgcctcgtac
      481 ctgaaagtcg ttaagtttca tgccctgtaa gtgtgacggt acttgcgaaa ccactttgat
      541 ctacgcgtgc tcgggagcgg cgaataccgg gttgctcgcc gaccaggtga tgcggatcct
      601 gagtcggaac aaggtcggct cgagcacctg cctggccgcg atgggcgccg acctctcggg
      661 cttccttgag tcggcgcgca acgcgacgcg caatatcgtg ctggacggct gcaaggtatc
      721 ctgcggcaag aggatattcg agaagaacgg cttagcgtac cagcactacg tcatgaccga
      781 ctacgacgtc gagaagggaa agacgcccat aaccggcgac ctgaccgagg ccgtggccga
      841 gcgtatagag gggctggtca aggcatgagc tccaaggaaa tctcccccaa ccagaaactg
      901 ctcatcgtct tcggcgtctt cctcgcggcg tacttcatac cgtggggcgc ggcgcgcgtc
      961 ctcggcgcga cgaacgaggc gttcatgatg ctcggcgagt atgctaggca gcacgtgctc
     1021 ctgtgcctcg taccggcgat gttcatagcc ggcgccatca ccgtgttcct gaaccagcag
     1081 gcggtgatgc gctacctcgg gcccgacgcc aaccggttca ccgcgtacgg cgtggcctcg
     1141 gtctccggcg cgatactcgc ggtctgctcc tgtaccgtat tgcccatctt caagggcatc
     1201 tacaagaagg gcgccggact gggtccggcg gtggcgttcc tgtattcagg tccagccatc
     1261 aatatcctcg ccatcgtact gtcggccaag gtcttcggct ggaagctcgg cgt
//
LOCUS       DAOPHV010000743         1312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_151316_length_1312_cov_2.597454, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000743 DAOPHV010000000
VERSION     DAOPHV010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151316_length_1312_cov_2.597454"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="PLQ29_13960"
     CDS             <1..1147
                     /locus_tag="PLQ29_13960"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPG87796.1"
                     /translation="RLGPDAVYDFSLGNPDLEPPAEFLAALKSAASSDELGSHAYMTN
                     AGYPFARAAMAEKVALEHGVPTTSDDVVMTVGAAGALNVILRTVVNPGDEVVVVRPYF
                     AEYSAYVDNCGGVLVVADSAPDFSLDPDAVARVLSPRTAAVIVNSPNNPTGRVYPASA
                     IAALAAVLSRHAAASGRAPYLVVDEPYRDIVYDGVAVPPVLSAYPDSVVASSFSKTLS
                     IPGERLGYLAVNPACSDKALLMAGLAASNRVLGFVNAPALMQRAVAASWRAKADVSRY
                     ERRRAMLSAVLDEAGIRYARPEGAFYLFCEAPAAAAARAATGESAATGESADVAFAMA
                     LKDRGVLSVPGVGFGYPGWIRLCYCVPEATIEGSRKAFLDTMEAYRG"
BASE COUNT          181 a          515 c          418 g          198 t
ORIGIN      
        1 caggctcgga ccggacgccg tctacgactt ctcgctgggc aaccccgacc tcgagccgcc
       61 ggccgaattc ctggcggcgc tcaagtccgc ggcgtcgagc gacgagctcg gctcgcacgc
      121 ctacatgacc aacgccggct atcccttcgc gcgcgccgcg atggccgaga aggtggccct
      181 cgagcacggc gtgccgacga cctccgacga cgtggtgatg acagtcggcg cggccggcgc
      241 cctcaacgtg atcctgcgca ccgtcgtcaa ccccggcgac gaggtcgtcg tcgtcaggcc
      301 gtatttcgcc gagtactccg cctacgtcga caactgcggc ggcgtcctcg tcgtcgccga
      361 ctcggcgccc gatttctcgc tcgacccgga cgccgtggcg cgcgtcctgt cgcccaggac
      421 cgcggccgtc atcgtcaaca gcccgaacaa cccgaccggc cgcgtctatc ccgcctcggc
      481 gatcgccgcg ctggcggccg tcctgtcgag gcacgccgcc gcctcgggcc gcgcgccata
      541 cctcgtcgtc gacgagccgt accgggacat cgtctacgac ggcgtggcgg tgccgccggt
      601 cctgtcggcc tatcccgaca gcgtcgtcgc ctcgagcttc tcgaagaccc tgtccatacc
      661 cggcgaacgg ctcggctacc tggccgtcaa ccccgcctgt tccgacaagg ccctgttgat
      721 ggccgggctc gccgcctcga accgcgtgct cggcttcgtc aacgcgccgg ccctgatgca
      781 gcgcgccgtg gccgccagct ggcgcgccaa ggcggacgtg tcgcgctacg agcgtcgccg
      841 cgccatgttg tccgcggttc ttgacgaggc cggcatacgc tacgcccgcc ccgagggcgc
      901 cttctacctg ttctgcgagg cgcccgccgc cgcggccgcg cgagccgcca cgggcgagag
      961 cgccgccacg ggcgagagcg ccgacgtggc cttcgccatg gccctcaaag accgcggcgt
     1021 cctgtccgta cccggcgtcg gtttcggcta cccgggctgg atacggctct gctactgcgt
     1081 ccccgaggcg accatagaag gctccaggaa agcgttcctg gataccatgg aggcgtaccg
     1141 aggttgaacg atcgggcggc gcctacccgc ataggcatcc ggcgcccgac acactccctg
     1201 ctacccggca cgacgcgccg ccccgagatc cttactcgta gccgggcctc gcataaacgc
     1261 gatcgccgac tgacgcggcg atcgccggcc gtcgttcgac ctgactcgct ac
//
LOCUS       DAOPHV010000744         1307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_152140_length_1307_cov_5.383387, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000744 DAOPHV010000000
VERSION     DAOPHV010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152140_length_1307_cov_5.383387"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /gene="pgi"
                     /locus_tag="PLQ29_13965"
     CDS             <1..1093
                     /gene="pgi"
                     /locus_tag="PLQ29_13965"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625632.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPG87797.1"
                     /translation="LERWAAERGELALEASFISNVDPDDANGVLSGCDQETSLFVLVS
                     KSGTTQETLANEALVRSWLGRRPGVDASRHIVAVTSETSPLARNAGYLDSFYIDDYIG
                     GRYSATSAVGAVVLSLALGPDAFKALLDGAADADRLALEPDPTKNAALLDAMLGIYER
                     NVLGRPSVAVLPYSQALARFPAHLQQLDMESNGKRVDRYGKPVTIPSGPAVFGEPGTN
                     GQHSFYQLLHQGTDIVPLQFIGFRKNQSGFDPEFQGSTARRKLNANLAAQIVAFACGK
                     DDDDPNKAFPGGRPSSLIVGDELDPGALGALLAHYENKVMFQGFAWNLNSFDQEGVQL
                     GKKLTGSVLADGGPSDPALAAYARLLGAL"
     gene            1090..>1307
                     /locus_tag="PLQ29_13970"
     CDS             1090..>1307
                     /locus_tag="PLQ29_13970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPG87798.1"
                     /translation="MSRDDEMTAGSASPRGGDSACIAFTGGGTGGHVFPGLAVIEALR
                     VRWPGRIVWIGSGKEVERQAVEAAGVEFV"
BASE COUNT          222 a          470 c          420 g          195 t
ORIGIN      
        1 cctcgaacgc tgggccgccg agcgcggcga attggcgctc gaggcgtcct tcatcagcaa
       61 cgtcgacccc gacgacgcga acggcgtgct gtccggctgc gaccaggaga cgagcctgtt
      121 cgtactcgtg tcgaaaagcg gcaccaccca ggagactctc gcgaacgagg ctctcgtccg
      181 gtcctggctc ggcaggaggc ccggcgtcga cgcgtcgcgg catatcgtcg ccgtaaccag
      241 cgagacgagc cctctggcgc gcaacgccgg ctacctcgat tcgttctaca tcgacgacta
      301 cataggcggc cgctacagcg ccacgagcgc ggtaggcgcc gtcgtcctat cgctggccct
      361 cggccccgac gccttcaagg ccctgctcga cggcgccgcc gacgccgacc gactggcgct
      421 cgagccggat cctaccaaga acgcggccct gctcgacgcc atgctcggca tctacgaacg
      481 gaacgtcctc ggccgcccga gcgtagcggt cttgccctac tcccaggcgc tggcgcgttt
      541 cccggcccac ctccagcagc tcgacatgga atccaacggc aaacgggtcg accgctacgg
      601 taagcccgtg acgatcccga gcggcccggc ggtattcggc gagccgggca cgaacggcca
      661 gcactcgttc taccaactcc tccatcaggg caccgatatc gtaccgctac agttcatcgg
      721 cttccgcaag aaccagagcg gcttcgatcc ggagttccag ggttcgaccg cgcggcgcaa
      781 gctgaacgcg aacctcgccg cccagatcgt cgccttcgcc tgcggcaagg acgacgacga
      841 ccccaacaag gccttccccg gcggccggcc ttcttcgttg atagtcggcg atgagctcga
      901 cccgggcgct ctcggcgccc tgctcgccca ttacgagaac aaggtaatgt tccagggctt
      961 cgcatggaac ctgaactcgt tcgaccagga gggcgttcag ctcggcaaga aactgaccgg
     1021 ctcggtactg gcggacggag gcccctcgga tccggccctg gcggcctacg cccgcctcct
     1081 aggcgcgcta tgagccgaga cgacgaaatg acggccggct ccgccagccc ccgcggtggc
     1141 gacagcgcct gcatcgcctt cacggggggc ggcaccggcg gccacgtatt ccccggcctg
     1201 gccgtcatcg aggccctgcg cgtgcggtgg ccgggcagga tagtctggat aggctccggg
     1261 aaggaggtag agcgacaggc ggtcgaggcg gctggggtcg agttcgt
//
LOCUS       DAOPHV010000745         1303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_152861_length_1303_cov_4.068109, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000745 DAOPHV010000000
VERSION     DAOPHV010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152861_length_1303_cov_4.068109"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PLQ29_13975"
     CDS             complement(<1..484)
                     /locus_tag="PLQ29_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPG87799.1"
                     /translation="MKKDILRLGIALALFAAGACAALAVVYTVTGPTIAGHEREQLQA
                     SLKDLFPDADGFVEIDGSIRSSVPDVTVLGSWKAARGDATLGVAIRASASSYGGDAVM
                     LVGVATDRRIAGARVMTLSDTPGLGANATNPSYFVDKAAKTTFPGQFSGKSVLDEFEV
                     K"
     gene            complement(481..>1303)
                     /locus_tag="PLQ29_13980"
     CDS             complement(481..>1303)
                     /locus_tag="PLQ29_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPG87800.1"
                     /translation="AVTKQDVRIGDLSAVVTGLLVALISPPSVPLWMVALGSFAAIVL
                     AKEFFGGLGANPFNPALIGRAILLMSFPAAMTTWHVPFKGLLDATTGATPLNVVKMGG
                     TIADVGAGFVQNSLSSVPDYSSTLWTLFVGNRAGCIGESSILLILVGFIFLLALRVVS
                     FITPLAMVGTAFLASWLLGMDPVFGILSGGLLFGAVFMATDYSSSPVTPLGKAIFGAG
                     AGLITVIIRRFGGYPEGVTYAILIMNAVAPFMDKLRVKKYGFVPPAKPVAKEAAK"
BASE COUNT          277 a          400 c          424 g          202 t
ORIGIN      
        1 tcttgacctc gaactcgtca aggacgctct ttccggagaa ctgccccggg aaggtggtct
       61 tagccgcctt gtcgacgaaa tacgaaggat tcgtcgcgtt cgcgccgagt ccaggggtat
      121 cggatagggt catcacgcgc gcaccggcga tacgacgatc ggtggcgact ccgaccagca
      181 tcacggcgtc accaccgtag ctcgaggcgc tagcgcggat ggccacgccg agcgtcgcgt
      241 cgccccgggc ggccttccac gagcccagga cggtcacgtc ggggacagag gatcgaatcg
      301 acccatcgat ctcgacgaac ccatccgcgt cgggaaagag gtccttcagc gaggcctgga
      361 gctgctcgcg ctcgtggccc gcgatggtcg gtccagttac ggtatagacg acggctaagg
      421 cggcgcacgc gccggcggcg aagagcgcca acgcgatgcc taacctgaga atgtccttct
      481 tcatttggcg gcctccttgg cgacgggctt ggccggaggc acgaatccgt atttcttgac
      541 ccggagctta tccatgaacg gcgccacggc gttcataatc aggatggcgt aggtcacgcc
      601 ttcgggatag ccaccgaagc ggcggatgat tacggttatc agccccgcgc cggcgccgaa
      661 tatcgccttg ccgagcggag tcaccgggct cgacgagtag tcggtagcca tgaataccgc
      721 gccgaatagg agtcctcccg ataggatgcc gaacaccggg tccatcccta gaagccacga
      781 ggctagaaag gcggttccga ccatcgccag cggggtaatg aaggagacca ctcggagagc
      841 gagcaagaag ataaaaccga ccaggataag gaggatcgag ctctcgccga tgcagccggc
      901 acggttaccc acgaacaacg tccacagcgt cgagctgtaa tcggggacag agcttagaga
      961 attctggaca aacccggcgc ccacgtcggc aatcgtccca cccatcttga cgacgttgag
     1021 cggcgtcgcg ccggttgtag cgtccagaag gcccttgaac ggtacatgcc aggtggtcat
     1081 ggccgcggga aagctcatca agagaatcgc gcggccgata agcgccggat tgaagggatt
     1141 ggcgccgagc cccccgaaga attccttggc cagtacgatg gcggcgaacg agccgagggc
     1201 caccatccac agggggacgg acggcgggct gatcagcgcg acgaggagac cggtcacgac
     1261 ggccgacagg tcgccgatac ggacgtcctg cttcgtgacc gcg
//
LOCUS       DAOPHV010000746         1303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_152864_length_1303_cov_3.905449, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000746 DAOPHV010000000
VERSION     DAOPHV010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152864_length_1303_cov_3.905449"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLQ29_13985"
     CDS             <1..292
                     /locus_tag="PLQ29_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HPG87801.1"
                     /translation="VLYSLIGLAFLLVAFIVFRLASREVKRRRRIAEEKRALEQQMLR
                     ENAIRQAEEQNIEVSMSVEERKRLDLQEHAINMAKEHPEDVAQLIRTWLREE"
     gene            296..>1303
                     /gene="fliG"
                     /locus_tag="PLQ29_13990"
     CDS             296..>1303
                     /gene="fliG"
                     /locus_tag="PLQ29_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HPG87802.1"
                     /translation="MPQAAPAKGKSTGPGASGSTGKKPKSAKDLNGRQKAAIFLITLG
                     SEISAEIFKHLREDEIETLTFEIARLENVEPELKDAILMEFQELMMASEFISSGGIDY
                     ARELLEKALGSQKAIDVINRLTSSLQVRPFDFIRRTDPANLLNFIQTEHPQTIALILA
                     YLEPNKASYILTKLPKEHQSDVAKRIATMDRTSPETLREVERVLEKKLSTLSSEDYTA
                     AGGVDAIVEILNLADRTTEKSILEALEEENPELAEEIKKKMFVFEDIVMLDNRSIQKV
                     IREVDTQDLTRALKGVDTEVQEKVFQNVSKRAAQALKEDMEYMGPVRIKDVEEAQQKI
                     VA"
BASE COUNT          306 a          406 c          405 g          186 t
ORIGIN      
        1 agtgttatac tcgctgatcg gcctggcctt cctgctcgtc gcgttcatcg tcttcaggct
       61 ggcctcgagg gaggtcaagc gcaggcgcag gatagcggag gagaagcggg ccctcgagca
      121 gcagatgctc agggagaacg cgatacggca ggcggaggag cagaacatcg aggtgtccat
      181 gtccgtggag gagcgcaagc gactggatct ccaggagcac gccatcaaca tggccaagga
      241 gcatcccgag gacgtggctc agctcatccg gacctggctc agggaggagt gacagatgcc
      301 ccaagccgcc ccagccaagg gcaagtcgac gggaccggga gcgagcggat cgaccggcaa
      361 gaagcctaaa agcgccaagg acctgaacgg tcgccagaag gccgccatct tcctcatcac
      421 cctcggtagc gagatatccg ccgagatctt caagcacctg cgcgaggatg aaatcgagac
      481 gctgaccttc gagatagcgc gcctggagaa cgtcgagccg gagctcaagg acgccatcct
      541 gatggagttc caggagctga tgatggccag cgagttcatc agctcaggcg gcatcgacta
      601 tgcccgcgag ctgctcgaga aagccctcgg ctcccagaag gcgatagacg tcatcaaccg
      661 cctgaccagc tccctccagg tcaggccgtt cgacttcatc cgccgtaccg acccggccaa
      721 cctgctcaac ttcatccaga ctgagcaccc gcagaccatc gccctgatcc tggcctacct
      781 cgagccgaac aaggccagct acatcctgac caagttgccc aaggagcacc agtcagacgt
      841 ggccaagcgc atcgcgacga tggatcgtac ctcgccggag accctgcgcg aggtggaacg
      901 cgtgctcgag aagaagctgt caacgctgtc gagcgaggac tataccgccg cgggcggcgt
      961 cgacgccatc gtcgagatac tcaacctcgc cgaccgcacc acggagaaga gcatcctcga
     1021 ggcgctcgag gaggagaacc ccgagctggc cgaggagatc aagaagaaga tgttcgtctt
     1081 cgaggacatc gtcatgctgg acaaccgctc gatccagaag gtcatccgcg aggtcgatac
     1141 ccaggacctc acgcgggccc tcaagggtgt cgacaccgag gtccaggaga aggtgttcca
     1201 gaacgtgtcc aagcgcgccg cccaggcact caaggaggac atggagtaca tgggcccggt
     1261 acgcatcaag gacgtcgagg aagcccagca gaagatagtc gcg
//
LOCUS       DAOPHV010000747         1303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_152899_length_1303_cov_2.942308, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000747 DAOPHV010000000
VERSION     DAOPHV010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152899_length_1303_cov_2.942308"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLQ29_13995"
     CDS             complement(<1..423)
                     /locus_tag="PLQ29_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPG87803.1"
                     /translation="MRIISRKRLAVAALLVGAALGANAQSAKQYEVTKPIKIEWWHAL
                     ESQYAPLVERVVADFERQNPLIDVEAIYQGSYKDLNEKLIAAHAAGVTLPAVTVANTP
                     YIAEYGASGVCEVLDPYIKATKFDLGDFGSGLKVASSYG"
     gene            complement(410..1231)
                     /locus_tag="PLQ29_14000"
     CDS             complement(410..1231)
                     /locus_tag="PLQ29_14000"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPG87804.1"
                     /translation="MSHIYYSELCLIGGDVSRNVDRLVEAGADGVELMLDGAGWDGVY
                     DRPGDYASALAGKGVAYSVHVPVWDANLTSENPRIREATAASYRFSIEFAALIGARHV
                     VLHPGYISDPRFSRHAAMERARDAVVALVSFNEAYGRSLLVENIGSRDSCIFTEGQYA
                     AFLNGFPPEVGYVVDVGHANVCGWRLESLLPALGPRLRALHLHDNDGRSDAHAPIGRG
                     TVDWASVMAAAGRGGADPILVLEYDVGTDLRRLAEGKAFLLGTTSTQGGEDDEDH"
BASE COUNT          211 a          423 c          441 g          228 t
ORIGIN      
        1 gccgtagctc gaggcgacct tgaggcccga gccgaagtcg cccaagtcga acttggtggc
       61 cttgatgtag gggtcgagca cctcgcagac gcctgaggcg ccgtactcgg cgatataggg
      121 cgtattcgcc acggtgaccg ctggcagcgt gacgccggcg gcgtgggcgg ctatcagctt
      181 ctcgttcagg tccttgtagc taccctggta gatcgcctcg acgtcgatca gtgggttctg
      241 gcgctcgaaa tcggctacga cccgctcgac gagcggggcg tactgcgact cgagcgcgtg
      301 ccaccactcg atctttatcg gcttggtgac ctcgtactgc ttggcgctct gggcgttcgc
      361 gccgagcgcc gctccgacga gcagcgccgc gacggcgagc ctcttacggc taatgatcct
      421 catcgtcttc tcctccttgg gtggacgtag tgcctaatag gaaagccttg ccctcggcaa
      481 ggcgcctaag atcggtgccg acgtcgtact cgagcacgag gatagggtcc gcccctcccc
      541 ggccggccgc ggccatgacg gacgcccagt cgacggtgcc gcggccgatg ggcgcgtgcg
      601 cgtcggagcg tccgtcgttg tcgtgcaggt gcagcgcgcg cagcctgggg ccgagggcgg
      661 gtagcaggga ctcgagccgc cagccgcaga cgttggcgtg gccgacgtcg acgacgtagc
      721 cgacctcggg cgggaagccg ttcaggaagg cggcgtactg gccctcggtg aagatgcacg
      781 agtcgcggga gcctatgttc tcgacgagca ggctacggcc gtaggcctcg ttgaagctta
      841 cgagggccac gacggcgtcc ctggcacgct ccatcgcggc gtggcggctg aaacgcggat
      901 cggatatgta gcccgggtgc aggacgacgt ggcgcgcgcc tatcagcgcc gcgaactcta
      961 tcgagaaccg gtacgaggcg gcggtcgcct cgcggatacg cgggttctcg ctggtcaggt
     1021 tggcgtccca gaccgggacg tgcaccgagt acgcgacgcc cttgccggcg agggcgctcg
     1081 cgtagtcgcc cggtcgatcg tagacgccat cccagccggc tccgtcgagc atcagctcga
     1141 cgccgtccgc gcccgcctcg acgagccgat cgacgttgcg cgagacatcg ccgccgatca
     1201 agcatagctc cgaatagtag atatggctca tcagtacccc gataccgcat acgtacccca
     1261 cggcactgtg gggctaatcc atcatgccat ctatttaata ttt
//
LOCUS       DAOPHV010000748         1299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_153584_length_1299_cov_4.377814, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000748 DAOPHV010000000
VERSION     DAOPHV010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153584_length_1299_cov_4.377814"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLQ29_14005"
     CDS             complement(<1..325)
                     /locus_tag="PLQ29_14005"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HPG87805.1"
                     /translation="MPVRLLALDLDDTLLTPELRIHPANLDAVRRALDSGVDVMLASG
                     RTVESMMPYAIELGMKGRGLPMICANGAEVRDVDSGELTRRLTLSADACRAAIEALSG
                     YGLPVQ"
     gene            457..>1299
                     /locus_tag="PLQ29_14010"
     CDS             457..>1299
                     /locus_tag="PLQ29_14010"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HPG87806.1"
                     /translation="MIAKRLLLALLSVSALALAGAQDAELAKALDAAASGYYLLEVRA
                     AFGTFTYEYSGLPTPFSRWVEDRALLAAAGSSRVRVLNRNAAAAMDPVLGATYADFIR
                     ETGAEALLSGRFFDEGGKARVRFELTELSSGILIGAGDWYVPAQAIPAYASVRPATGV
                     LDRSRELSRLSGSTPGGLSVSVSTDRGAGAAYRVGEALTLLVGVSDDAYARVYHVDGT
                     GRVQLIWPNRFGGGDGHIAAGSPVRLPPDESAPYAFVMEPPYGTEFIKVVASTAPFGD
                     GLEDF"
BASE COUNT          177 a          424 c          486 g          212 t
ORIGIN      
        1 cctggaccgg gaggccgtag ccggacaggg cctcgatcgc cgcccggcag gcgtcggccg
       61 acagggtgag cctgcgcgtc agctcgcccg aatcgacgtc gcggacctcg gcgccgttgg
      121 cgcagatcat cggcaggccg cggcccttca tgccgagctc gatggcgtag ggcatcatcg
      181 actcgacggt ccgccccgag gcgagcatca cgtcgacgcc cgagtcgagg gcgcgtcgca
      241 ccgcgtcgag gttggccggg tggatgcgta attcgggggt caggagcgtg tcgtcgaggt
      301 cgagggcgag tagtcgtact ggcatgtcga ttgtatacca tgggtgcggc gggcgtcaca
      361 aggacgggcc cggtacggat gggcggccgg gaggatgcgc cgtttcgccg ggcgcgatat
      421 actctacggc gtgttcgtgg tcgggatgga ggtcgcatga tagctaaaag actgctgctc
      481 gcgctgttgt ccgtatcggc cctggccctg gcgggggccc aggacgccga gctggccaag
      541 gccctggacg ccgcggcgtc tggatactat ctactggagg tcagggccgc gttcggcacc
      601 ttcacctacg agtattcggg cctgcccacg ccgttctcgc gctgggtcga ggaccgcgcc
      661 ctgctcgccg cggccggctc gtccagggtc cgcgtactca accgcaacgc cgccgccgcg
      721 atggaccccg tgctcggcgc cacgtacgcc gacttcatcc gcgagacggg cgctgaggcg
      781 ctgctgtcgg ggcgcttctt cgacgagggc gggaaggccc gggttcgttt cgagctgacc
      841 gagctgtcga gcggcatcct gataggcgcc ggcgactggt acgtgccggc ccaggcgata
      901 cccgcctacg cctccgtgag gccggccacg ggcgtgctcg ataggtcgcg cgagctatcg
      961 cggctgtcgg gctccacgcc cggcggcctg tccgtgtcgg tgagcaccga ccgcggcgcc
     1021 ggcgccgcgt accgggtcgg cgaggcgctg acgttgctag tgggcgtcag cgacgacgcg
     1081 tacgcgcgcg tctaccacgt ggacggcacc ggtagggtgc agttgatctg gccgaaccgc
     1141 ttcggaggcg gcgacggcca tatagcggcc ggctcgccgg tgcggctgcc gccggacgag
     1201 tcggcgccct acgccttcgt gatggagccg ccctacggta ccgaatttat aaaagtggta
     1261 gcgtcgacgg cgccgttcgg cgatggcctc gaggatttc
//
LOCUS       DAOPHV010000749         1296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_154160_length_1296_cov_3.311845, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000749 DAOPHV010000000
VERSION     DAOPHV010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154160_length_1296_cov_3.311845"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PLQ29_14015"
     CDS             <1..758
                     /locus_tag="PLQ29_14015"
                     /inference="COORDINATES: protein motif:HMM:NF021610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HPG87807.1"
                     /translation="EDREVDGHELFDAPVAAGRPLATRRVPAQGDRYRLFFRYAKEGR
                     AAFFAHHELQAMLSSAIDRAGAPIAYSEGFNPMPRLEISEPMSLGFESSDEYGSVILT
                     GRLPVDPSDVASAANRLLHGDLRILEAYTLLCQDGKKFPSLSSVHWGSRYSVDLLSTG
                     LDPRLLASAVRKRIQSDTRLADATVGVDGDVVTLLLPFTGKRELGLPALFEDAYGFSI
                     REARISVRRSAQYAKSADGGVETYADRYSQYRC"
     gene            complement(892..>1296)
                     /locus_tag="PLQ29_14020"
     CDS             complement(892..>1296)
                     /locus_tag="PLQ29_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87808.1"
                     /translation="YLASSSYLGYQRLVLNGESSLPTIHKDYLTDDYYITVELPSGGA
                     REPYLTIVDVATASIVRSYVIRRAETGSDNAYLTFLDEPKPGAADYRSSTNDTDDYYY
                     VQFYAAAYGIDLNDFSNLFGDAISIGRLRLSF"
BASE COUNT          254 a          392 c          386 g          264 t
ORIGIN      
        1 aggaagatcg ggaggtcgac gggcacgaat tgttcgacgc gcccgtagcc gccggccggc
       61 cgctggctac tcgtcgcgtc ccggctcagg gagaccggta tcggttgttc ttccggtatg
      121 cgaaggaggg cagggcggcc ttcttcgcgc accatgaact ccaagccatg ctcagctccg
      181 ccatcgaccg cgccggtgct ccgatagcgt actccgaggg cttcaacccg atgcctcgcc
      241 tcgagatctc cgagcccatg tccctcggtt tcgagtcgag cgacgagtac ggctccgtca
      301 tattgaccgg ccggctcccg gtcgatccct ccgacgtagc gtctgccgcc aaccgcctcc
      361 tgcatggaga cctcaggata ctcgaggcct atacgctgct atgccaggat ggcaagaagt
      421 tcccatccct gtcttccgtc cactggggtt cgcgctacag cgtcgatctc ctgagtaccg
      481 gcttggatcc gaggctactc gcatcagcag tacggaaacg tatacagtcc gatacgaggc
      541 ttgccgatgc caccgtcggc gtcgatggcg atgtcgtgac gctcttgctg ccattcacgg
      601 gcaagcgcga gttaggcctg ccggcgctct tcgaggatgc gtacggattc tcgatccgcg
      661 aagccaggat atcggtccgt cgttcggccc agtacgccaa gtcggccgac ggcggtgtcg
      721 agacgtacgc cgatcgctac tcgcaatacc gatgctaggc agcgccgcat aagcttcgtg
      781 acaggtatgc ggcataatgg aaggcgagct ggtagacaga atatacatta tcggaagtcg
      841 tcagtattcg atagggccac ggtcaagcga tccctggact cgtccctatc gctagaacga
      901 tagccttagc ctgccgatcg agatggcgtc tccgaagagg ttgctgaagt cgttcaagtc
      961 tataccatag gccgccgcgt agaattggac gtaataatag tcgtcggtat cgttcgtgct
     1021 cgacctatag tcagcggcgc ccggcttggg ttcgtcgagg aacgtcaggt acgcattgtc
     1081 cgacccggtc tctgcccgcc tgatcacgta ggagcggacg atactggcgg tagccacgtc
     1141 gacgatcgtc aagtacggtt ccctggcgcc gccagaaggc aactccacgg ttatatagta
     1201 atcgtccgtc aggtagtctt tatggatagt cggcaaggac gactctccgt tcagcacgag
     1261 ccgctgataa ccaaggtacg acgacgaggc cagata
//
LOCUS       DAOPHV010000750         1294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_154604_length_1294_cov_2.185634, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000750 DAOPHV010000000
VERSION     DAOPHV010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154604_length_1294_cov_2.185634"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PLQ29_14025"
     CDS             complement(<1..679)
                     /locus_tag="PLQ29_14025"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPG87809.1"
                     /translation="MHVLTTNDTRFQAVDSANARAWDILSAQPQSALEEAERALATAS
                     EAGYAKGVADAHLNIGWSMYYLTHLPEAYRAFLEVHREYLALGDVLGVCKTLNALGVY
                     HYSIFRLEKAVDYFTQSLEVAKANGIEDRELIVGINIGELCLLLGNPQDALEYLIPAY
                     VRMIGVLPPSNVADCLRNIGKAFLRLDNPALAAEYIRRSYSLAKDVGESIMATDSVET
                     LADVAIES"
     gene            819..>1294
                     /locus_tag="PLQ29_14030"
     CDS             819..>1294
                     /locus_tag="PLQ29_14030"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPG87810.1"
                     /translation="MSIVTRTGDEGMTGLWSGERVRKEDPRVEAYGTVDELDSVIGEA
                     KHWLKRVDCRRIAEELQRDLYRVAGQLATRTGSFSKPMTDADEVRLASYVGAFEDEIP
                     LTGFVVPGSTLQSAKLDVARTVCRRAERRIVALSRDADVPGQVIRYMNRLSDLLFM"
BASE COUNT          231 a          380 c          445 g          238 t
ORIGIN      
        1 cgctctcgat ggccacatcg gccagggtct cgacgctgtc ggtcgccatg atggactcgc
       61 ctacgtcctt ggcgaggctg tacgagcggc gtatgtactc ggccgccagg gccgggttgt
      121 caagccgcag gaaggccttg ccgatgttgc gcaggcagtc ggcgacgttg ctcggcggca
      181 acacgcctat catcctgacg taggcgggta tcaggtattc gagggcgtcc tgcggattgc
      241 cgagcaacag gcagagctcg ccgatgttta tgccgacgat gagttcccgg tcctcgatgc
      301 cgttcgcctt ggctacctcg aggctctgcg tgaaatagtc cacggccttc tcgaggcgga
      361 aaatcgagta gtggtagacg cccaaagcgt tcagcgtctt gcagacgccg agtacgtcgc
      421 ctagggcgag gtactcgcga tggacctcca ggaaggcgcg ataggcctcg ggcaggtgcg
      481 tcaggtagta catggaccag ccgatattga ggtgggcgtc ggcgacaccc ttggcgtaac
      541 cggcctcgga cgccgtcgcc aaagcccgct cggcttcctc gagggccgac tggggctggg
      601 cgctcaagat atcccaggcg cgagcgttgg ctgagtcgac ggcttggaag cgggtatcgt
      661 tcgtggtcaa tacgtgcacc tattcaagaa tattactatg gatcgggcga ttgtatccag
      721 atcattgtag cacgagttgg cgccgctgag cgtgctatat atgctactat acgcgcgccg
      781 ccaaacgcgg cgcgacggag acggggcgag gagcgttcat gagcatcgtt acgaggaccg
      841 gggacgaggg aatgaccggg ttatggtcgg gcgagcgggt ccgcaaggag gatccccgcg
      901 tcgaggcgta tggcaccgtg gacgagctgg actcggtcat cggcgaggcc aagcactggc
      961 tcaagcgcgt cgactgcagg cgtatcgccg aggagctgca acgcgatctg taccgggtag
     1021 ccgggcaact ggccactcgt accggctctt tctccaagcc catgacggac gcggatgagg
     1081 tcaggctggc atcctacgtc ggggccttcg aggacgagat accgctgacc ggcttcgtcg
     1141 tacccggctc gacgctccag tcggccaagc tcgacgtggc caggaccgtc tgcaggcggg
     1201 cggagcggcg catcgtcgcg ctgtcccgcg acgcggacgt gcccggccag gttatccgct
     1261 acatgaaccg gctgtcggac ctgctgttca tgat
//
LOCUS       DAOPHV010000751         1293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_154764_length_1293_cov_2.522617, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000751 DAOPHV010000000
VERSION     DAOPHV010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154764_length_1293_cov_2.522617"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1293)
                     /locus_tag="PLQ29_14035"
     CDS             complement(<1..>1293)
                     /locus_tag="PLQ29_14035"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HPG87811.1"
                     /translation="RADVEVARSATAPAEGLLSEEAAEGLLSEEPASPVEASSFWSQA
                     GVDEEAALDALLARMGPDERLGQLFMLSYGGEEPTPLLYSWIRRRGLGGVKIFGWNAD
                     DTGKLAAAIEAIQNAAENGPLDVPPFVATDQEGGWIRHVRGSTSVTPGNMAIGASGWP
                     SDAYRSAYYIGVELAALGVTMNFAPTVDLATRPESTIIGPRAFSSDPTETGILAAAWA
                     RGMADAGVLATAKHYPGHGHTELDSHGILPVIDIDEETLWNRELVPYRMLAAEGVPGV
                     MSGHLAFPRISGSNEPASLSRHFIQELLRERMGYDGLVITDDLMMSGAASPGGLSETC
                     ELAIRAGNDILMFSRTLALDDAAWARLARLYRSDASFKARVDESARRVLRAKLRWLLP
                     LGRDGVRPDDDAEAAMRSEESRAFFAEQAMRSATVLGRS"
BASE COUNT          172 a          448 c          477 g          196 t
ORIGIN      
        1 ggagcggccg agcaccgtgg cgctgcgcat cgcctgctcg gcgaagaacg cgcgtgattc
       61 ttcgctgcgc atcgcggctt ccgcgtcgtc gtcgggcctg accccgtctc ggccgagtgg
      121 caggagccag cggagcttgg cgcgtagcac gcgcctggcc gactcgtcga ctcgcgcctt
      181 gaacgaggcg tcgcttcggt acagcctggc caggcgggcc caggcggcgt cgtcgagcgc
      241 gagcgtgcgg ctgaacatca ggatgtcgtt gccggcgcgg atcgccagct cgcaggtctc
      301 ggacaggccg ccggggctgg ccgccccgct catcatcagg tcgtcggtga tcacgaggcc
      361 gtcgtagccc atccgctcgc gcagcagctc ctggatgaag tgtcgggaca gggaggctgg
      421 ctcgttcgag ccggatatcc tcgggaaggc caggtgcccg ctcatcacgc cgggtacgcc
      481 ctcggcggcc agcatgcggt acggcaccag ctcgcgattc cagagcgtct cctcgtcgat
      541 gtcgatgacc ggcaggatgc cgtgggagtc gagttcggtg tggccgtggc cggggtagtg
      601 cttggcggtc gccaggaccc ccgcgtcggc catgccccgg gcccaggcgg ccgcgaggat
      661 gccggtctcg gtcgggtcgg acgagaaggc gcgcgggccg atgatggtcg attccgggcg
      721 cgtggccagg tcgaccgtcg gcgcgaaatt catcgtgacg ccgagggccg cgagctcgac
      781 gcctatgtag taggcgctcc ggtaggcgtc gctcggccag ccggaggcgc ctatggccat
      841 gttgccaggc gtcacgctag tcgaaccgcg cacgtgcctg atccagccgc cctcctggtc
      901 ggtggcgacg aacggcggta cgtcgagggg gccgttctcg gcggcgttct ggatggcctc
      961 gatggcggcg gccaacttgc ccgtgtcgtc ggcgttccag ccgaagatct tgacgccgcc
     1021 gagcccgcgc ctacgtatcc acgagtatag gagcggcgtg ggctcctcgc cgccgtacga
     1081 cagcatgaac agctggccga ggcgctcgtc gggccccatg cgcgccagga gggcatcgag
     1141 cgccgcctcc tcgtcgacgc cggcctgcga ccagaacgac gacgcctcga ccgggctggc
     1201 cggctcctcg gacaggagcc cctcggccgc ctcctcggac aggagcccct cggccggggc
     1261 cgtcgccgag cgagcgacct cgacgtcggc ccg
//
LOCUS       DAOPHV010000752         1292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_154883_length_1292_cov_3.928052, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000752 DAOPHV010000000
VERSION     DAOPHV010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154883_length_1292_cov_3.928052"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..1076
                     /gene="speD"
                     /locus_tag="PLQ29_14040"
     CDS             276..1076
                     /gene="speD"
                     /locus_tag="PLQ29_14040"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HPG87812.1"
                     /translation="MVRRIKGQTIKLDGFNNLTKSLSFNIYDICYARSTESQIEYLEY
                     IDEEYNSARLGTIVEEVTRIINAKLVQVSTQDYEPRGASVVALLNEDQYPAHTTPAPG
                     VLGHLDKSHIAIHTYPEYNQLSGIATFRVDVDIATCGMISPLSALHYLIESLESDVVI
                     IDYRVRGFTRDSRGVKLFIDHDISSIQDFIAEDALDDYTAYDVNIVSDNNFHTKMRRK
                     GLKLKDYLFGDEVTSLPPKEKRRIRQLLDLEVQEIFECKNLGRRMGRP"
     gene            1073..>1292
                     /locus_tag="PLQ29_14045"
     CDS             1073..>1292
                     /locus_tag="PLQ29_14045"
                     /inference="COORDINATES: protein motif:HMM:NF036419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87813.1"
                     /translation="MTANCRDDGRDAATGGHRELRERLNAHAGMFYDIGRVIVEARTP
                     YQDAELVETETFGRVLLLDGVTQVSERWE"
BASE COUNT          266 a          435 c          375 g          216 t
ORIGIN      
        1 gctcgttatg gcaggccatc aggctcgagc cgacgagcgc ccgcccgccg tccttctgga
       61 acgtcgccgc cgacttgtcg ttcatgtaga ctatcctgtg atcgcggtcc gataccgtca
      121 ccgcgccggg gaacccggtt acccaatcgg gcatgtcgct catattcgct cctcgctcgc
      181 cgcattgccg gcgcccggca actgtaccaa agcgccaggc cgggttacaa tggccgttcg
      241 ctcatcacca tgagattcga gggggcacgg gcacattggt acggagaatc aagggacaaa
      301 cgatcaagct ggacgggttc aataacctga cgaaatcttt aagcttcaac atctacgaca
      361 tctgctacgc caggtcgacc gaatcgcaga tcgagtacct cgagtacatc gatgaggagt
      421 acaactccgc caggctcggg accatcgtcg aggaggtgac gcgcatcatc aacgccaagc
      481 tcgtgcaggt ctcgacccag gactacgagc cgcgcggggc cagcgtcgtc gccctgctca
      541 acgaggacca gtatccggcg cacacgaccc ccgcgcctgg cgtcctgggc cacctggaca
      601 agagccacat agcgatccat acctatcccg agtacaacca gctgtccggc atcgccacct
      661 tcagggtcga cgtcgacata gccacctgcg gcatgatcag cccgctgtcc gccctccact
      721 acctgatcga gagcctcgag tcggatgtcg tcatcatcga ctaccgagtc cgcggcttca
      781 cgcgcgacag ccgcggcgtc aagctgttca tcgatcacga tatatcgtcg atccaggact
      841 ttatcgcgga ggacgccctc gacgattaca cggcctacga cgtcaacatc gtctccgaca
      901 acaacttcca taccaagatg cgccggaagg gtctcaagct caaggattac ctgttcggcg
      961 acgaggtgac gagcctgccg cccaaggaga agcggcgcat caggcagctc ctcgacctcg
     1021 aggtgcagga gatcttcgaa tgcaagaacc tcggccgccg catgggacgc ccatgacggc
     1081 gaactgccgc gacgacgggc gcgatgcggc gaccggcggt catcgcgagc tgcgcgagcg
     1141 cctcaacgcc catgccggca tgttctacga catcggccgc gtgatcgtcg aggcgcgcac
     1201 tccgtaccag gacgccgagc tcgtcgagac ggagaccttc ggccgcgtgc tgctcctcga
     1261 cggcgtcacc caggtgtcgg agcgatggga gg
//
LOCUS       DAOPHV010000753         1286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_156019_length_1286_cov_4.248578, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000753 DAOPHV010000000
VERSION     DAOPHV010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156019_length_1286_cov_4.248578"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="PLQ29_14050"
     CDS             <1..908
                     /locus_tag="PLQ29_14050"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPG87814.1"
                     /translation="RAEVQKLKPMVRGLIGVLMGVSGFCSKVLRVPAGRVLLAFIRKK
                     AGLGSVRLGVAGGGPLALDTAEFFDTIGFNLVQGYGMSENGPLIAVNLPEYKDNRSVG
                     LPVKRTLVRIADPGPDGVGEIQVQSPSMMLGYLDAPEATRAVITDDRWLRTGDLGYVD
                     RRGFIFITGRSKNIIVTEGGKNVYPEEIELRFEGSAWINEALVLGRKVSGLNAGEQVI
                     AVCVPNWDRVDAERGGVPRDEFALERVRDEVRRVNKSLPSFMKIIDFIVRNDEFEKTS
                     TRKIRRFLYADYGRAPAPERPEVGR"
     gene            905..1204
                     /locus_tag="PLQ29_14055"
     CDS             905..1204
                     /locus_tag="PLQ29_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87815.1"
                     /translation="MSLDIAALALRFAVMAVATFLAIIVWSRVRDVSWMLIVVGIIAG
                     YGDILYSLLVEFGLVPDIGEAMGGLSFLAFVAPNLPWVFFSIAFITMLARRRSRP"
BASE COUNT          213 a          399 c          430 g          244 t
ORIGIN      
        1 tacgagccga ggtgcagaag ctgaagccca tggtccgcgg cctcatcggc gtcctgatgg
       61 gcgtctcggg gttctgctcc aaggtcctgc gcgtgccggc cggacgcgtc ctcctcgcct
      121 tcatccgcaa gaaggccggc ctcggttcgg tcaggctcgg cgtcgccggc ggagggccgc
      181 tggcgctcga tacggcggag ttcttcgaca ctatcggctt taacctggtc cagggctacg
      241 ggatgagcga gaacgggccg ctcatcgcgg tcaacctgcc tgagtacaag gacaaccgct
      301 cggtgggtct gccggtcaag cggacgcttg tgcgtatagc cgatcccgga cccgacggcg
      361 tcggagagat ccaggttcaa agcccctcga tgatgctcgg ctacctcgac gctccggaag
      421 caacccgagc ggtcataacg gatgaccgat ggctgcgtac cggggacttg ggctacgtcg
      481 ataggcgggg tttcatcttc atcactggcc gctccaagaa catcatcgtc accgagggcg
      541 gcaagaacgt gtatcccgag gagattgagt tgcgcttcga gggctcggcc tggataaacg
      601 aggcgttggt gctcggccgc aaggtctccg gcctcaacgc gggcgagcag gttatagccg
      661 tctgcgtgcc gaattgggac agggtcgacg ccgagcgcgg aggggtgccg cgcgacgaat
      721 tcgcgctcga acgcgtacgc gatgaggtgc gccgggtcaa caagagcctc ccatcgttca
      781 tgaagatcat cgactttatc gtacggaacg atgaattcga gaagacgagc acccgtaaga
      841 tcaggcgctt cctgtacgcc gactacgggc gcgccccggc gccggagcgg cccgaggtcg
      901 gccgatgagc ctcgatatcg ccgccctggc gctgcgcttc gcggtgatgg cggttgctac
      961 cttcctggcc atcatcgtgt ggtcgcgcgt gcgcgacgtg tcgtggatgc tcatcgtcgt
     1021 cggcatcatc gccgggtatg gagacatcct ctactcgtta ttggtcgaat tcggcctcgt
     1081 gccggatatc ggggaggcga tgggcggctt gagcttcctg gccttcgtcg ctccgaacct
     1141 cccgtgggta ttcttctcga tcgccttcat taccatgctg gcccggagac gctccaggcc
     1201 ctgatcccgc aagcgccgag gccccgccgc gcgtatggcg aggtggaggc cggccatgac
     1261 agtctacgcg ttcgcgccga taggct
//
LOCUS       DAOPHV010000754         1285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_156244_length_1285_cov_3.082114, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000754 DAOPHV010000000
VERSION     DAOPHV010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156244_length_1285_cov_3.082114"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(322..>1285)
                     /gene="eno"
                     /locus_tag="PLQ29_14060"
     CDS             complement(322..>1285)
                     /gene="eno"
                     /locus_tag="PLQ29_14060"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPG87816.1"
                     /translation="GVSMAVSRAAADYLDVPLYKYLGGAHTTLLPVPMSNIINGGKHA
                     DNKIDFQEFMIMPVGAESFREAVRMNAEVFHNLKSLLKSDGHNTSVGDEGGFAPNLGN
                     EEALDYIMKAIEKAGYRPGEQIAIALDCASSELFDEGDKKGYKFWKSNPGKLFSADEM
                     IEIYTKWVAKYPIVSIEDGLDQNDWEGYVKLTKALGGKIQIMGDDFFVTNTARLEKGI
                     AMGACNSILIKLNQIGTVTETIEAINMAKRAGYTAVVSHRSGETEDTFLADLTVACET
                     GQIKTGSMSRTDRIAKYNQLMRIEDAMEGIAEYPGMKAFYNIKR"
BASE COUNT          208 a          395 c          427 g          255 t
ORIGIN      
        1 cggtctaccc cccggacgac gacgcggccg ccgagaagac ccggcgcctc gtcgacctgg
       61 gccgcgccaa ggtcgccgcc ccggctgact actcggaagg ctcgagcctc gagctgcgcg
      121 tccaggcgat cgtcgatcat atcgaggcgg gttgccggat acgatgagcg ccagaggtct
      181 cgacggccga acctggcggg cctagcctcg ccgacagtgc catacagccc gattccagcg
      241 cctcgcgcgg gaacgcgcgc caaaaaaacc gccccccgag gggggcggcc acggatcgcc
      301 cacgcgcggg cgggatgcta cttacctctt gatgttgtag aacgccttca tccccggata
      361 ctccgcgatg ccttccatcg cgtcctcgat gcgcatcagc tggttgtact tggcgatgcg
      421 gtcggtgcgc gacatcgagc cggtcttgat ctggccggtc tcgcaggcga cggtgaggtc
      481 ggcgaggaag gtgtcctcgg tctcgcccga gcggtgcgag acgacggcgg tatagccggc
      541 gcgcttggcc atgttgatgg cctcgatggt ctccgtgacg gtaccgatct ggttgagctt
      601 aatgaggatg gagttgcagg cgcccatcgc gatgcccttc tccaggcgcg cggtattggt
      661 gacgaagaag tcgtcgccca tgatctggat cttgccgccg agggctttcg tcagcttaac
      721 gtagccttcc cagtcgttct ggtccaagcc atcctcgatg gagacgatgg ggtacttggc
      781 gacccacttg gtgtagatct cgatcatctc gtcggcgctg aaaagcttgc cggggttcga
      841 cttccagaac ttgtagccct tcttgtcgcc ctcgtcgaac aactcggaag aggcgcagtc
      901 gagcgcaatg gctatctgct cgccggggcg atagccggcc ttctcgatgg ccttcatgat
      961 gtagtcgagg gcctcctcgt tgccgaggtt cggagcgaag ccgccctcgt cgccgacgga
     1021 ggtgttgtgt ccgtcggact tgaggaggct cttgaggttg tggaagacct cggcgttcat
     1081 gcgcacggcc tcgcggaagc tctcggcgcc gaccggcatg atcatgaatt cctggaagtc
     1141 gatcttgttg tcggcgtgct taccgccgtt gatgatgttg gacatcggca cgggcaggag
     1201 cgtcgtgtgg gcgccgccca ggtacttgta gaggggtacg tcaagatagt cggcggccgc
     1261 cctggagact gccatcgaca cgcca
//
LOCUS       DAOPHV010000755         1284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_156517_length_1284_cov_2.225386, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000755 DAOPHV010000000
VERSION     DAOPHV010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156517_length_1284_cov_2.225386"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLQ29_14065"
     CDS             <1..417
                     /locus_tag="PLQ29_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87817.1"
                     /translation="AIVCAAVSSCSFMGISGSSVPAGTYRAITDPDGDHVVTTVELAD
                     GSFKYQRAIDGDVQVRYEATFDSLTDIGNNTYEAVLTSLTGLAGSTDWTSLNTSVAQL
                     QLIYNSPTLRVNLDVNGNGAFDAGLVDLVEVSFEAE"
     gene            404..1087
                     /locus_tag="PLQ29_14070"
     CDS             404..1087
                     /locus_tag="PLQ29_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87818.1"
                     /translation="MRRSDARTVPFRRLVVALLLATMMAAPALYAQDAPANAVSIDPL
                     LIIFNMYKGTYELRVADQFSAMAEVAYTPNFFWGIHQYTKLTYLDLIGKGRYYVGSLL
                     KGAADDIGNEKIRGYVGRAFTDALGGVFVGGFLGYVNSVVEQGAGADYFRADFNGFGG
                     GLELGLKYIFGDSGLSFFAEPYVLLRYYAGGYAYRDESGVTIVKPEGFADGFDRNGIA
                     AGVNIGVSF"
BASE COUNT          238 a          422 c          388 g          236 t
ORIGIN      
        1 gcgatcgtat gcgcggccgt gtcctcctgc tcgttcatgg gcatcagcgg cagttccgtg
       61 ccggccggca cgtatagggc cataacggat cccgacggcg accacgtggt aaccacggtg
      121 gagctcgccg acggtagctt caagtatcag cgggcgatag acggcgacgt ccaggtacgg
      181 tacgaggcca ccttcgatag cttgaccgac atcggtaaca atacctacga ggccgtactg
      241 accagcctga ccgggctagc aggctcgacc gactggacta gcctgaacac ctccgtcgcg
      301 cagctgcagc tgatctataa cagccccacg ctcagggtta acctcgacgt caacggcaac
      361 ggcgccttcg acgccggcct cgtcgacctg gtggaggtaa gctttgaggc ggagtgacgc
      421 acgaacggtt ccctttaggc gactggtcgt cgcgctcctg ctggcgacca tgatggcggc
      481 cccggcgctc tacgcccagg acgccccggc taacgccgtg tcgatagacc cgctcctgat
      541 catcttcaac atgtacaagg gcacctacga actgcgagtg gctgaccagt tctcggctat
      601 ggccgaggtg gcgtacacgc cgaatttctt ctggggcata caccagtaca cgaagcttac
      661 ctatctggac ctgataggca agggccgcta ttacgtcggc agccttctca agggcgcggc
      721 cgacgacatc gggaacgaga agatacgcgg ctacgtcggc cgcgcgttta ccgacgccct
      781 cgggggcgta ttcgtcggcg gtttcctggg ctacgtcaac tcggtcgtgg agcagggcgc
      841 cggcgcggat tacttccggg ccgacttcaa cggcttcggg ggcggtctcg aactcggcct
      901 caagtacatc ttcggcgata gcggcctgag cttcttcgcc gagccgtacg tactgctacg
      961 ctactacgcg ggcggctacg cctaccgcga cgagagcggc gtgactatag tcaagcccga
     1021 aggcttcgcc gacggcttcg accgcaacgg catcgccgcc ggggtcaaca tcggcgtatc
     1081 gttctagtct tccctgcggc cgcccgggcg ccttgtcgtc ccggcggccg cgttctcccg
     1141 cggcgccgat ctcccggaac ttgacgacct cgccatcctg ccccacactc atcgcatcga
     1201 ctctatatac agaaaggatg catgaatgga tagcaagcta cggatcctgg tcgtcaacgg
     1261 cagccccaag ggcgactata gcct
//
LOCUS       DAOPHV010000756         1276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_157953_length_1276_cov_2.549550, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000756 DAOPHV010000000
VERSION     DAOPHV010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157953_length_1276_cov_2.549550"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="PLQ29_14075"
     CDS             complement(<1..954)
                     /locus_tag="PLQ29_14075"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPG87819.1"
                     /translation="MRTQNVLEMRGICKSFPGVRALHDVSFTLRAGEVHALMGENGAG
                     KSTLIKVMTGVYEKDSGQIAIEGNAIHFKSPQDAQAFGVGTVYQEITLCPNLTVAENM
                     FIGRGRYAFVNWKRMRERAARLLDSLGIPARPTQQLSSCSLAIQQMIAIARAIDMDCK
                     ILILDEPTSSLDEDEVRKLFSLMRELKSRGVGIVFVTHFLDQVYEISDRITVLRNGEF
                     IGEYEASALPQFELVSKMMGKALDDVARIRKHEPSTRDGETPVFEATGLSSAAGVRPF
                     DFSIRKGEVNGFTGLLGSGRSESVRAIYAADKVTGGDIKIKG"
     gene            complement(1061..>1276)
                     /locus_tag="PLQ29_14080"
     CDS             complement(1061..>1276)
                     /locus_tag="PLQ29_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPG87820.1"
                     /translation="VQAGYWDCDIQCSPRQAAEISGWIKTLESGKKSPTGIVYQKEIY
                     ADTATITDELILSKGINADPGKGVITK"
BASE COUNT          219 a          410 c          372 g          275 t
ORIGIN      
        1 gcccttgatc ttgatgtcgc cgccggtaac cttatcggcc gcgtagatcg cgcggacgct
       61 ctcgctgcgc ccggagccga gcaaaccggt gaacccgttc acttcgcctt tccgtatgga
      121 gaagtcgaag ggcctgaccc ccgccgcgct cgatagcccc gtcgcctcga acaccggcgt
      181 ctcgccgtcg cgagtcgaag gctcatgctt ccgtatcctc gcgacgtcgt cgagcgcctt
      241 acccatcatc ttcgatacca gctcgaactg gggcagggcc gacgcctcgt actcgcctat
      301 gaattcgccg ttgcgcagca ccgtgatcct gtcgctgatc tcgtatacct ggtccaggaa
      361 gtgggttacg aagacgatgc ccacgcccct ggacttcagc tcgcgcatca gcgagaagag
      421 cttgcgaacc tcgtcctcgt cgagcgacga ggtcggctcg tccaggatga ggattttgca
      481 gtccatgtcg atggcgcggg cgatcgctat catctgctgg atggccagcg agcagctcga
      541 cagctgctgg gtcggcctgg ccgggatgcc gagcgagtcc aggagcctag cggcccgttc
      601 cctcatccgt ttccaattga cgaaggcgta gcggcccctg cctatgaaca tgttctccgc
      661 gacagtcagg tttgggcaga gtgtaatctc ctggtacacg gtgccgaccc cgaaggcctg
      721 agcatcctgg ggcgacttaa aatggatggc gtttccttct atcgctatct ggccggagtc
      781 cttctcgtag acgccggtca tcaccttgat caaggtggac ttgccggcgc cgttctcccc
      841 catgagggcg tgaacctctc ccgcccgaag ggtgaagctg acgtcgtgca gggccctgac
      901 cccgggaaac gacttgcata tgccccgcat ctcaagcaca ttctgcgttc gcactcgttc
      961 ctctccctga tgcatcgaga ataaaaacgt gcggcgcggc aagtctgact atacccagcg
     1021 ccgcgccgca cgcctcaagc ggttctatga ctatatacgg ttacttcgtg atcacgccct
     1081 tgcctggatc ggcattgatg cccttgctca ggatgagctc gtcggtgatc gtggccgtat
     1141 cggcgtagat ctctttctga tagacgatgc cggtggggct cttcttgccg gattccaggg
     1201 tcttgatcca accggagatc tcggccgcct ggcgggggct gcactggatg tcgcaatccc
     1261 agtagccggc ctgtac
//
LOCUS       DAOPHV010000757         1274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_158307_length_1274_cov_3.105004, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000757 DAOPHV010000000
VERSION     DAOPHV010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158307_length_1274_cov_3.105004"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLQ29_14085"
     CDS             complement(<1..472)
                     /locus_tag="PLQ29_14085"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HPG87821.1"
                     /translation="MKFVFFGAPGAGKGTMAARAAVAFGLPHISTGEIFRAAMRAGTP
                     LGLRVKAVIESGGLVSDELTIGLVRERLGEADAARGWLLDGFPRTLAQAEALAGFCPE
                     DFVIDLEVSDAKVVERLSGRRMCRGCGRSFHVSLMPPAEEGVCDGCGGELYVRDD"
     gene            complement(545..>1274)
                     /locus_tag="PLQ29_14090"
     CDS             complement(545..>1274)
                     /locus_tag="PLQ29_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87822.1"
                     /translation="LEPVDLSDFPQVIRFPRYEIRAALFIGSFDPFQMTHLATALRFL
                     ASAAAGAPVVFVVPEGHDNPRKPRKSDYRYRYELLSMQVAGVFDPLIVPLDIGEGADT
                     IGIVRRFIAMFPGARVSMTHLLGSDTLPFAASMLPEDLNAWRQESERRRVDFTYEAFV
                     VTREGSPVSASDVEAIRSRGLPIQVDDEPIGTPSSTDFRENRAFSIVFPTESVIRHME
                     VLFRYNLNKPWSVCGLDEGPGRTR"
BASE COUNT          234 a          432 c          400 g          208 t
ORIGIN      
        1 catcatcccg gacatagagc tcgccgccgc agccgtcgca gacaccttcc tcggctggag
       61 gcatcaggct gacatggaag ctgcgcccgc agccgcggca catccgccgc ccggacaggc
      121 gctcgacgac cttggcgtcg ctcacctcaa ggtcgatcac gaagtcctcc gggcagaacc
      181 cggccagggc ctcggcctgg gccagagtcc tcgggaaacc gtcgagcaac cagcccctcg
      241 ccgcgtcggc ctcgccgagg cgctcgcgaa cgaggccgat ggtcagctcg tcggacacga
      301 gaccacccga ttcgatgacg gccttgaccc tgagcccgag cggcgttccg gcgcgcatgg
      361 ccgccctgaa gatttcgccc gtcgagatat gcggcaggcc gaacgccacc gccgccctgg
      421 ccgccatggt gcctttaccc gctccaggcg ctccgaagaa tacgaatttc atagtcgacc
      481 cgctccttgc gcgctatatg ccagcatcct atctcaagtc gcgggcgcgc gctaggccgt
      541 acggctaccg agtccggccg gggccctcgt ccaggccgca cacgctccac ggcttgttca
      601 ggttgtaccg gaacagcacc tccatgtgcc tgatcaccga ttcggtaggg aacacgattg
      661 aaaacgcccg attctccctg aaatccgtgc tggatggcgt accgatgggc tcatcgtcga
      721 cctggatagg tagtccgcgg ctcctgatcg cctcgacgtc ggatgccgag actggagagc
      781 cttccctggt cacgacgaag gcctcgtacg tgaaatcgac gcgcctacgt tcagattcct
      841 ggcgccatgc gtttaggtcc tcgggcagca tcgacgccgc gaacggcagc gtatccgaac
      901 ccaggaggtg ggtcatcgag acccgagcgc cggggaacat cgcgatgaag cgcctgacga
      961 tgccgatggt gtcggctccc tcgcctatgt ccagcggcac gatcagcggg tcgaagactc
     1021 cggctacctg catcgataga agctcgtacc gatagcgata gtcgctcttc ctgggctttc
     1081 tcgggttatc gtggccctcg ggcacgacga acacgacggg cgctccggcg gcggccgagg
     1141 cgaggaacct gagcgccgtg gccaggtgcg tcatctggaa ggggtcgaag gagccgatga
     1201 ataacgcggc gcggatctcg taacgcggga agcgtatcac ctggggaaaa tcggacaggt
     1261 ccaccggctc gagc
//
LOCUS       DAOPHV010000758         1271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_158848_length_1271_cov_4.127467, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000758 DAOPHV010000000
VERSION     DAOPHV010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158848_length_1271_cov_4.127467"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="PLQ29_14095"
     CDS             <1..173
                     /locus_tag="PLQ29_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011193502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HPG87823.1"
                     /translation="NQDNPLTNGLRPILGLDVWEHAYYLKYQNRRQEYIDNFFNVIAW
                     KEVSRRFDAANT"
     gene            224..>1271
                     /locus_tag="PLQ29_14100"
     CDS             224..>1271
                     /locus_tag="PLQ29_14100"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPG87824.1"
                     /translation="MVYDYDLHTIADIAPIGAAEFRDRPALGLVGGEHVDYRELELQS
                     RRVAADLYRRGVRKGDRVAILSESRPEWGVASLGIARTAAVAVPLLVDFSSSQLREIL
                     AHAKASIVYASTKQRPKLEGLAITALPIEDLTSMSAELDRDAAELELPRPTPDDLAAI
                     VYTSGTTGNPKGVMLTHRNYISDALACSAIIVLNPRDVLLSILPLAHAYEYTIGFLIP
                     MMSGAFVQYLDRPPSASALMPALASVRPTIVLSVPLVIEKTYRQAVKPALEKMRAYRN
                     PVLRPILTRLAGAKLYRAFGGRLRFFGVGGAPLDPEVERFLQMVRWPYAIGYGLTETA
                     PIIAASAVGKTFPKA"
BASE COUNT          233 a          427 c          387 g          224 t
ORIGIN      
        1 agaaccagga caacccgctc acgaacggcc tacgccctat actcggcctg gatgtctggg
       61 agcacgcgta ctaccttaag taccagaacc gtaggcagga gtacatcgat aacttcttca
      121 acgtgatcgc ctggaaggag gtctccaggc gattcgacgc tgcgaatacc taaaccatgc
      181 aaccatcctg gatcccggcg gtctaactcg ccgaggatcc aagatggtat acgactacga
      241 cctacatacg atcgccgaca tcgccccgat cggggcggcg gaatttagag acaggccggc
      301 gctcggcctc gtcggtgggg aacacgtcga ctatcgcgag ctcgagctcc agtcgaggcg
      361 cgtggctgcc gacctgtatc gccgcggcgt ccgtaagggc gaccgcgtgg ccatcctatc
      421 ggagagccga cccgagtggg gcgtcgcgtc gctcggtata gccaggacgg ccgcggtcgc
      481 cgtgccgctc ctcgtcgatt tctcctcgtc ccagctacgc gagattctcg cccatgctaa
      541 agccagtata gtctacgctt cgacgaagca acggccgaag ctcgagggcc tcgccatcac
      601 ggccttgccg atagaggatc tgacgagcat gtccgcggag cttgaccgcg atgcggccga
      661 gctcgagctg ccgagaccga ctccggacga tcttgcggcc atcgtctata cctccggcac
      721 gaccggcaat cccaagggcg tgatgctgac ccaccggaac tatatctcgg acgcgctcgc
      781 ctgttcggcc atcatcgtac tgaaccctcg cgacgtgctc ctgtccatac tgcccttggc
      841 ccacgcctac gagtatacga taggcttcct gatcccgatg atgagcggcg ccttcgtcca
      901 gtacctggac cggccgccgt ccgcgtcggc cctgatgccg gcgctcgcgt cggtacggcc
      961 gaccatcgtg ctgtcggtgc cgctggtgat cgagaaaacc tataggcagg cggtcaagcc
     1021 ggcgctcgag aagatgcgag cctaccggaa tcccgtcctg cggccaatcc tgacgcgcct
     1081 ggccggagcc aagctgtacc gggccttcgg cgggcggctg cgcttcttcg gcgtcggcgg
     1141 agcgccgctc gacccggagg tcgagcgctt cctccagatg gtcaggtggc cgtacgccat
     1201 aggctatggg ctcaccgaga cggccccgat catagcggct tcagcggtcg gcaagacctt
     1261 tcccaaggcg g
//
LOCUS       DAOPHV010000759         1267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_159634_length_1267_cov_4.013201, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000759 DAOPHV010000000
VERSION     DAOPHV010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159634_length_1267_cov_4.013201"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1267)
                     /locus_tag="PLQ29_14105"
     CDS             complement(<1..>1267)
                     /locus_tag="PLQ29_14105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG87825.1"
                     /translation="LEPRGVSATLGPDSIPVSLWIHPVGEAGSEGAVLTAVDLRAEFA
                     NARTKRRLADADRLLDEFIARSLDGIVLTDADGLIVRWNYPMVAMTGIEAEEALGSHY
                     WDALTMVQPESGFEPARMRRAIRAMLAMRDPARHRQVLETVIWRRDGSRRIVQNDLFP
                     IPLADGTILAIIARDVTDERRLAEENIERIRKLDHAQKMEAVGTLSGGIAHDFNNTLA
                     GIVGAASLIRQGIESGAVTTAADVERELELIERASKRAASSVRRLMTLTRKRSPESLV
                     FRLDEALRRVEEFAARSMDPSVSLSLRHGMPEAWVRGDSGQLEQLFLNLIVNAEHAVT
                     IMRPREQRRGGSVVISLYRFAPDRDFLIANPNVAEAEYWALSVKDDGVGVPRHIQRRI
                     FDPFYTTKPNESSSGLGLAMVHAIATQHGG"
BASE COUNT          192 a          423 c          431 g          221 t
ORIGIN      
        1 gccgccgtgc tgcgtggcga tggcgtggac catggccagg ccgagcccgc tcgaggactc
       61 gttcggctta gtcgtgtaga acgggtcgaa gatgcgcctc tggatatgcc tgggcacgcc
      121 gactccatcg tccttgaccg atagggccca gtactccgct tccgcgacgt tcgggttggc
      181 tatcaggaag tccctgtcgg gcgcgaagcg gtacagggag atgacgacgc ttccgccccg
      241 ccgctgctct ctcggcctca tgatggtgac ggcgtgctcg gcgttcacga taaggttgag
      301 gaacagttgc tcgagctggc ccgagtcgcc gcgcacccaa gcctcgggca taccgtggcg
      361 cagcgatagc gagaccgatg gatccatcga gcgcgcggcg aattcctcga cccgcctaag
      421 cgcctcgtcc agcctgaaaa cgagcgactc gggcgaccgc ttacgggtca gcgtcatgag
      481 gcggcgtacc gacgaggcgg cgcgcttcga ggcgcgttct atcagctcga gctcccgctc
      541 gacgtcggcg gcggtcgtca cagcgccgct ctcgatgccc tggcgtatga gcgaggcggc
      601 tccgacgatg ccggcgagcg tgttattgaa gtcgtgggct atgccgccgg atagcgtgcc
      661 gaccgcctcc atcttctgcg cgtggtcgag cttacggatg cgctcgatat tctcttcggc
      721 caggcggcgc tcgtcggtca cgtcccgcgc gatgatcgcc aggatggtac cgtcggccaa
      781 cgggatgggg aacaggtcgt tctgaacgat gcgcctggag ccatcccgcc tccagatgac
      841 cgtctcgagc acctgcctat gccgggccgg gtcgcgcatg gccagcatgg ccctgatggc
      901 cctcctcatg cgcgccggct cgaagccgct ctccggctgc accatcgtca gcgcgtccca
      961 gtagtggctg ccgagagcct cctcggcctc gatgcccgtc atcgcgacca tcgggtagtt
     1021 ccaccggacg atgaggccgt cggcgtcggt cagcacgatg ccgtcgagcg acctggcgat
     1081 gaactcgtcg agcagtcggt cggcgtcggc gagcctgcgc ttggtgcgcg cattggcgaa
     1141 ctcggctcgc aggtcgaccg cggtcagtac ggcgccctcg gagccggcct cgccgaccgg
     1201 atggatccac aggctgacag gtatagaatc gggtccgagc gtcgccgaga cgccgcgcgg
     1261 ctcgagc
//
LOCUS       DAOPHV010000760         1265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_160039_length_1265_cov_3.752893, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000760 DAOPHV010000000
VERSION     DAOPHV010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160039_length_1265_cov_3.752893"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLQ29_14110"
     CDS             <1..347
                     /locus_tag="PLQ29_14110"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPG87826.1"
                     /translation="FQIMDDVLDYESTEGELRKPVGNDVREGLCTLPLILALEAGAPG
                     LRPLLGPAAPNDDSVAAIVAAVRDSGALSSAKARAATYTDRAMRELASLPRGRAREDL
                     HAIVGRLLTRTF"
     gene            357..932
                     /locus_tag="PLQ29_14115"
     CDS             357..932
                     /locus_tag="PLQ29_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPG87827.1"
                     /translation="MLTYILAGFALAMDAFAVSVSSGVCVSGLTVTYAVRTAFSFGLF
                     QFLMPLAGYAIGSTFAAAIGRWDHWLAFGLLATVGGKMVIESVGAVKDASCTDEDRRK
                     LNVLDLRVLLVLSIATSIDAMAVGIGYSVIGAPIWIASSIIGGITFALCLIGCEFGKR
                     IGAWLERWAELAGGLVLIGIGVKILLEHLLA"
     gene            complement(1116..1262)
                     /locus_tag="PLQ29_14120"
     CDS             complement(1116..1262)
                     /locus_tag="PLQ29_14120"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPG87828.1"
                     /translation="MADVRLAAGAGFIYPLSGEINTMPGLSSKPGYIGMDIDTDTGRI
                     IGLS"
BASE COUNT          219 a          423 c          383 g          240 t
ORIGIN      
        1 cgttccagat catggacgac gtgctggact acgagtcgac cgagggcgag ctgcgtaagc
       61 cggtaggcaa cgacgtacgc gagggcctgt gtaccctgcc actgatcctc gcactcgagg
      121 caggcgcacc cggcctgcgg ccattgctcg gcccggccgc cccgaacgac gactcggtag
      181 cggccattgt tgcggcggtc agggactcgg gcgcgctcag ctcggccaag gccagggccg
      241 ccacctacac cgacagggcc atgcgagaac tcgcgagcct tcctaggggt agagcccgcg
      301 aagaccttca tgctatcgtc ggacgcctac tgacccgtac cttctgaggt gtatccatgc
      361 tcacgtacat cctggccggc ttcgcgctgg ccatggacgc gttcgccgtt tccgtatcga
      421 gcggcgtctg cgtctccggc ctgaccgtca cctacgccgt ccgcaccgcg ttctccttcg
      481 gcctcttcca gttcctgatg cccctagccg gttatgccat cggatcgaca tttgccgccg
      541 ccatcgggcg ctgggaccat tggctggcct tcggcctact cgccacggta ggcggcaaga
      601 tggtcataga gtccgtcggc gctgtcaagg acgcatcgtg taccgacgag gaccgccgca
      661 agctcaacgt gctcgacctg cgggtgctgc tcgtactgtc catagcgacg agcatcgacg
      721 cgatggccgt aggcatcggc tacagcgtga taggggcgcc gatctggata gcctcgtcga
      781 tcatcggcgg catcaccttc gcgctgtgcc taatcggctg cgagttcggc aagcgcatag
      841 gcgcctggct cgagcgctgg gccgagctgg ccgggggcct cgtcctcatc ggcatcggag
      901 taaaaatcct cctcgaacac ctcctcgcct aaccgtttac gataagccag cgaagtgcat
      961 aagaggacaa gagagaaagg ggaagcaggg gacgaggcga gccgatccac gtcgccgcct
     1021 cgcccgctcc atcccccagc cattgccgta tttcttctcc ggctttcgct cttttactcg
     1081 tatcctctac gcctaattag cctaccgtgc ccctatcaag ataggcctat gatgcggccg
     1141 gtatccgtat cgatatccat gccaatgtag ccgggcttgc tcgacaggcc gggcatcgtg
     1201 ttgatctcgc cggacagcgg gtagatgaaa ccggcacctg ccgcgaggcg gacgtccgcg
     1261 atcgg
//
LOCUS       DAOPHV010000761         1262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_160654_length_1262_cov_3.533554, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000761 DAOPHV010000000
VERSION     DAOPHV010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160654_length_1262_cov_3.533554"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PLQ29_14125"
     CDS             <1..356
                     /locus_tag="PLQ29_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87829.1"
                     /translation="PKNLKIALATAVADMRWQAAKEKASYYWMEEGLTGQYYQWFVKQ
                     KLKGDVKEFFIQDYITWITKESDGIQKLDKEVRGIFWRLMPFSQPIKDKLRDRSFVYQ
                     ELYQRDINRTLSDGY"
     gene            complement(350..1261)
                     /locus_tag="PLQ29_14130"
     CDS             complement(350..1261)
                     /locus_tag="PLQ29_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPG87830.1"
                     /translation="MATTKKTAILLATLTAFIWGLSFLSIKTAVAVIPPITLGLARFV
                     VADVVLLAAFAIMRKRPRLAPKDLPLMASAGLVGVTLYFLGENNGVLLLSASEASIIV
                     GTIPVITMLAERLFTKALLSPWQYAGAAASALGVALIVVESLRLSPAPLGYLYMALAA
                     LSWVAYAFMTKPLLGTYEPLEVTFWQSVFGAAGFVPFALVERTVWSAVTPVVMLNVLY
                     LGIFCSAVGYLFYVASLKALGAGVSSVFINLIPVVSVAASFVILGERLSPIQLGGGAV
                     AIGGVYLASFSGGRASARREPAQKWRQ"
BASE COUNT          263 a          403 c          415 g          181 t
ORIGIN      
        1 accccaagaa cctgaagata gccctagcca ccgccgtcgc ggacatgcgt tggcaggcgg
       61 ccaaggagaa ggcctcgtac tattggatgg aggagggcct gacgggccag tactaccagt
      121 ggttcgtcaa gcagaagctc aagggcgacg tcaaggagtt cttcatccag gactacatca
      181 cctggatcac caaggagagc gacggcatcc agaagctcga caaggaggtg cgcggcatct
      241 tctggcggtt gatgccgttc tcccagccca tcaaggacaa gctacgcgac cgcagtttcg
      301 tctaccagga actgtaccag cgcgacatca accgcacctt atccgacggt tattgacgcc
      361 acttctgggc gggctcccgc cgcgcgctcg cgcggccgcc ggagaagctg gccaggtaga
      421 cgccgccaat ggcgaccgcg cctccgccca gctggatggg gctcagccgc tcgcccagta
      481 tgacgaacga cgccgcgacg gacaccaccg gtatcaggtt gatgaacacg ctcgacacgc
      541 cggccccgag cgccttgagg ctcgctacgt agaacaggta gccgaccgcc gagcagaata
      601 tacccaggta cagcacgttg agcatgacga ccggagtgac ggccgaccag acggtcctct
      661 cgacgagggc gaacgggacg aaccccgcgg ccccgaacac gctttgccag aacgtcacct
      721 cgaggggctc gtacgtgccg agcaggggct tcgtcatgaa ggcgtaggcc acccaggaca
      781 gggccgccag ggccatgtac aggtagccga gcggggccgg gctcaggcgt agcgactcga
      841 ccacgatcag ggcgacgccc agggccgatg ccgccgctcc ggcgtactgc caaggcgaca
      901 ggagcgcctt ggtgaacagc cgctcggcga gcatcgttat caccggtatg gtgccgacga
      961 tgatcgacgc ctcggaggcc gacaggagca gtacgccgtt gttctccccg aggaagtaca
     1021 gcgtcacgcc gaccaatccg gcgctggcca tcagtggcag gtctttcggc gcgagcctgg
     1081 gccgcttgcg catgatggcg aaggcggcga gtagcacgac gtcggcgacg acgaagcggg
     1141 ccaagccgag cgtgatgggc gggatcacgg ccaccgccgt cttgatgcta aggaacgaaa
     1201 ggccccagat gaaggccgta agcgtggcga gcagtatcgc cgtcttcttg gttgtcgcca
     1261 tg
//
LOCUS       DAOPHV010000762         1260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_161115_length_1260_cov_2.511203, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000762 DAOPHV010000000
VERSION     DAOPHV010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161115_length_1260_cov_2.511203"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1042
                     /locus_tag="PLQ29_14135"
     CDS             176..1042
                     /locus_tag="PLQ29_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87831.1"
                     /translation="MVTYSYDIFAIVLCSAVCLAALSMETARSFHRRPLLYASWFASS
                     AALLACLPLSVIAPWPRAASALRAVALLSIAPAIISGALVLLLFIRRRPDDLVKRHEG
                     LLLDSIREGIVVFDAAGRIISIGRENPLPEVFVLDHRPDPPADHPAAFLSALLAAPEE
                     AAGTARLDSRSFYWRFKPLPGGRGSLLTLLDTSREQELADSLERAGAALAARQRLLVS
                     IERLDSEAARARIVEHVSGEIDREVRVKLKRFLSYAARNRSLSDCLGLAEESLAEVRS
                     LVNELAPRREAP"
     gene            1039..>1260
                     /locus_tag="PLQ29_14140"
     CDS             1039..>1260
                     /locus_tag="PLQ29_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPG87832.1"
                     /translation="MNCRIIFAEDQQLIRESVHLLLDGDPELSILDTAADGREALELC
                     RAKRPDIILMDVRMPVMDGREARLQIRNAE"
BASE COUNT          195 a          441 c          389 g          235 t
ORIGIN      
        1 gtatgtatac gtcgggcggc accatggcgg aagtgccgta ggtcacgtcg gccgccattc
       61 tttagtcagg ctcggtggca tgaccaaagg cacgatggac aaagacggcg actggcgttt
      121 ttttttaaac ccggcgtttg cagaccgcga ttcatggtct atacttgccc agcccatggt
      181 cacctacagc tacgacatct tcgcgatcgt tctctgctcc gccgtctgcc tcgccgccct
      241 ctcgatggag acggctcgtt cctttcaccg gcggccgctc ctgtacgcat cctggttcgc
      301 ctcgtcggct gcattgctcg cgtgcctgcc tttgtccgtt atagccccct ggccgcgcgc
      361 ggcatccgcc ttgcgagccg tcgccctcct gtcgatcgcg ccggcgatca taagcggggc
      421 gctcgtactg ctgctcttca tccgtcgccg tcccgacgac ctcgtcaagc gccatgaagg
      481 cctcctgctc gacagcatcc gcgagggcat agtcgtcttc gacgcggcgg gcaggatcat
      541 ctcgatcgga cgggagaacc cgttgccgga agtcttcgtc ctggaccata ggcctgaccc
      601 gccggccgac catccggcgg ccttcctttc ggcgctcctc gccgcccccg aggaggccgc
      661 cggcaccgcg cgcctggact cccgaagctt ctactggcgc ttcaagccgc tgccgggcgg
      721 ccgcggctcg ctcctgaccc tcctcgacac cagccgcgaa caggaactgg cggactcgct
      781 ggagcgcgcc ggcgccgccc tggccgcgcg ccagcggctc ctggtatcga tcgagcggct
      841 ggacagcgag gcggcgcggg cccgtatcgt cgagcacgtc tcgggcgaga tagaccgcga
      901 ggtgcgcgtg aagcttaagc gattcctgtc ctacgcggca aggaatcgat cgctctcgga
      961 ctgcctcggg ctcgccgagg aatcactcgc ggaggtacgt tcactcgtca acgagctagc
     1021 gccgcgcagg gaggctccat gaactgccgt atcatcttcg ccgaggatca gcagctcata
     1081 cgcgagtcgg tgcatctcct gctcgacggc gaccctgagc tgtccatact cgacaccgcg
     1141 gcggacggcc gcgaggcgct cgagctatgc cgcgccaagc ggccggacat catactcatg
     1201 gacgtacgca tgccggtcat ggacgggcgt gaagctcgcc ttcagatcag gaatgcggag
//
LOCUS       DAOPHV010000763         1260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_161154_length_1260_cov_2.184232, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000763 DAOPHV010000000
VERSION     DAOPHV010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161154_length_1260_cov_2.184232"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLQ29_14145"
     CDS             complement(<1..295)
                     /locus_tag="PLQ29_14145"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPG87833.1"
                     /translation="MKRSDNAPSVDRKTADELLYGSLRAVYHFERSLVERFGLGYQEI
                     YLLQLLRRREIARVGEIADALGAKMFTVSRLVKRLQERGYVSKAQDSGDRRGIL"
     gene            complement(407..>1260)
                     /gene="hcp"
                     /locus_tag="PLQ29_14150"
                     /gene_synonym="priS"
     CDS             complement(407..>1260)
                     /gene="hcp"
                     /locus_tag="PLQ29_14150"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPG87834.1"
                     /translation="HSEMLPAQYYPAFKKYPHFAGNYGNAWWKQREEFEQFRGAILMT
                     TNCIVPPADSYKARMYTTGVAGYPGCPRVEADASGHKDFSALIAAAKACQPPVEIETG
                     TIVGGFAHDQVAALAPKVVEAIKAGKIRKFVVMAGCDGRQKGRDYYREFAEALPKDAV
                     ILTAGCAKYRYNKLGLGDIDGIPRVLDAGQCNDSYSLVVTALLLKDAFGLASVNELPL
                     AFNIAWYEQKAVIVLLALLSLGVRNIHLGPTLPAFLSPNVAKVLVDAFGIAGIGAVED
                     DIKLLVG"
BASE COUNT          206 a          389 c          415 g          250 t
ORIGIN      
        1 cgagtatgcc cctgcggtcg cccgagtcct gggccttgct gacgtagccg cgctcttgca
       61 gccgcttgac tagccgcgat acggtgaaca tcttcgcacc gagagcgtcg gctatctcgc
      121 cgacgcgggc tatctctcgt ctacgcagca gctgtaggag gtatatttcc tgatagccga
      181 gcccgaagcg ctccaccagg ctccgctcga aatggtacac cgcgcggagg ctcccataga
      241 gcaactcgtc ggctgtcttc ctgtcgacgc tcggggcgtt atccgatctt ttcatggttt
      301 taatatagcg cctaatggag ctgaatgtga aacgctccga gccagcgtat cttggatacg
      361 ccagcccggg gcggaccgtg ctatcaggct atacgcgcct cggggatcag cctacgagca
      421 gcttgatgtc gtcttcgacc gcgccgatgc cggcgatgcc gaaggcgtcg acgaggacct
      481 tggccacgtt cggcgacagg aaggccggga gtgtcggccc caggtggatg ttcctgacgc
      541 cgagcgacag cagggccagg agcacgatga cggctttctg ctcgtaccag gctatgttga
      601 acgcgagcgg tagctcattc acgctggcca ggccgaaggc gtccttaagg agcagcgccg
      661 tgacgacgag cgagtacgag tcgttgcact gaccggcgtc gagcacgcgt gggatgccgt
      721 cgatgtctcc gaggccgagc ttgttgtagc ggtacttggc gcagcccgcc gtgagtatca
      781 ccgcgtcctt cggcagggct tcggcgaatt ccctatagta atcgcggccc ttctgacggc
      841 cgtcgcagcc cgccatcacg acgaacttgc ggatcttgcc cgccttgatc gcctcgacga
      901 ccttgggcgc gagcgcggcg acctggtcat gggcgaagcc tccgactatc gtgccggttt
      961 ctatctccac cggcggctgg caggccttgg cggcggcgat gagcgctgag aagtccttgt
     1021 ggccggaggc gtccgcttcc acgcgcggac agccgggata gccggcgacg cctgtcgtgt
     1081 acatcctggc cttgtaggag tcagccggcg gcacgatgca gttggtcgtc atgaggatgg
     1141 cgccgcggaa ctgctcgaat tcctcgcgct gcttccacca cgcgttgccg tagttcccgg
     1201 cgaagtgcgg gtacttcttg aaggccgggt agtactgggc ggggagcatc tcgctgtggg
//
LOCUS       DAOPHV010000764         1258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_161517_length_1258_cov_2.375727, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000764 DAOPHV010000000
VERSION     DAOPHV010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161517_length_1258_cov_2.375727"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PLQ29_14155"
     CDS             complement(<1..544)
                     /locus_tag="PLQ29_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HPG87835.1"
                     /translation="MALKKTELYSTIWKSCDELRGGMDASQYKDYVLILLFIKYVSDR
                     YSGDPKALIDIPAGGSFADLVALKGKANIGERINVIIAKLAEANGLKGVIDVADFNDA
                     DKLGKGKDMVDRLSNLVALFDNPNLDFRNNRACGDDLLGDAYEYLMKNFATESGKSKG
                     QFYTPAEVSRIMAKVIGVGDA"
BASE COUNT          248 a          379 c          376 g          255 t
ORIGIN      
        1 tggcgtcgcc cacgccgatg accttggcca tgatgcggct gacctccgcg ggcgtgtaga
       61 actgcccctt ggacttgccg ctctcggtgg cgaagttctt catcaggtac tcgtaggcgt
      121 cgcccaggag atcgtcgccg caggcccggt tgttacggaa gtccaggttc gggttgtcga
      181 acagggccac caggttggac aggcggtcca ccatgtcctt gcccttgccg agcttgtcgg
      241 cgtcgttgaa gtcggccacg tcgataacgc ccttgagccc gttggcctcg gccagcttgg
      301 ctatgatgac gttgatcctc tcgccgatgt tggccttgcc cttgagcgcc accaggtcgg
      361 cgaagctgcc gccggcggga atatcgatca gggcctttgg gtcgcctgag tagcggtcgg
      421 acacgtattt tatgaagagc aggataagga cgtagtcttt atactgcgag gcgtccatgc
      481 cgccgcgcag ctcgtcgcag gatttccaga tggtgctgta cagctctgtc tttttcagtg
      541 ccacgattta cctcaatgat gcgaaactgc cccattctag tgcccgaacc accccctggc
      601 aacagcaata agccatcgcg cgcaggcgtt cagtaaaagg tttcgcgcag agacgctaag
      661 taagtggccg gtacgactta atgcagaaca tgatgacagg atggatcagg atgaacacga
      721 tgggatggag gggtaaagac taggctctcg ttacgatgcc caaccctccc gatgctgaag
      781 ctatcggaag atgatgggga caggatctac atgataaagc gcggctatcg ggcgccatgc
      841 tagcttcgac cgaatcgcaa accagatact gagcacagcc cgctacccgg cacgacgcgc
      901 cgccccgaga tccttgctcg tacccaggcc tcgcatacac gagatcgccg gccgtcgttc
      961 gacctgactc gcgatgctcg caggtcaacg gcggtgcaca aaccgagctg attaccggtg
     1021 cctgatagac agcccgctac ccggcacgac gcgccgcccc gaaaaccttg ctcgtaccca
     1081 ggcctcgcat acacggggag gcgggtgtcc agaaagcgtc gtccctcgcg tagcgaggga
     1141 cacgcttcct ggacaggccc tgccgagatg atatacgagg cctgtataca ttcctgagcc
     1201 ggcatggggc ggcgcgtcgt gttagcgcga cgccccgatc gggtctattt tctttttt
//
LOCUS       DAOPHV010000765         1257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_161799_length_1257_cov_1.810316, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000765 DAOPHV010000000
VERSION     DAOPHV010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161799_length_1257_cov_1.810316"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1257)
                     /locus_tag="PLQ29_14160"
     CDS             complement(<1..>1257)
                     /locus_tag="PLQ29_14160"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPG87836.1"
                     /translation="ITQQDIDDAEVRLRPVLGIAPRLYVAGLYGLGLIAAAFMILLYP
                     GLRRPGASYSFKADPPGSAVFIDGAYVGYTPCELFVPAGEHELRVSRPGFADHSASMT
                     TKGRVFGTLIVKPREELFVSLEGADVEAVLSGGIERYASWALAGTPSESYQIPMDLSD
                     AVRAAGVVGDRADGLLGAAASYARHSQSLRDAARAASIAYGGSAAVTPASAGRLVAAV
                     IGELRDDPAILTVLAALGPETVRRRLESTGAYRAALGASDAVVEPVAGRSSMFAGEEF
                     VPMGGGVSIVKAGSALPAVSQLRPFWLASSETTVGRYRRFVAERPEWAPSAAASLAAE
                     GLADDAYLTGFGEAADSDPLRYVSKPAAEAYCAWLSAYAPAGYRFALPTEAQWSYAAA
                     TSGASATMGAVLLDGGYDAPLAPALLR"
BASE COUNT          201 a          419 c          474 g          163 t
ORIGIN      
        1 cgcaggagag ccggggcgag cggcgcgtcg tatccgccat cgagcagcac ggcgcccatg
       61 gtggcgctgg cgcccgaggt ggcagccgcg tacgaccact gagcctcggt cggcaacgcg
      121 aaacggtagc cggctggcgc gtacgcggat agccaggcgc aataggcctc ggcagcgggt
      181 ttcgatacgt agcgtagcgg atcggagtcg gcagcctcgc cgaaaccggt aaggtacgcg
      241 tcgtccgcga ggccttcggc ggccaaggac gcggccgccg aaggcgccca ttccggccgc
      301 tccgctacga agcgcctata acgcccgacg gtcgtctcgg acgaagcgag ccagaagggc
      361 cttagctggc tgacggcggg aagggccgag ccggccttaa cgatcgaaac gccgccgccc
      421 atggggacga attcctcgcc ggcgaacatc gacgagcgcc cggcgaccgg ttcgacgacg
      481 gcgtctgacg ctccgagcgc ggctcggtag gcgccggtcg actcgagccg ccgcctgacc
      541 gtctcgggcc cgagcgcggc gagcacggtc aggatcgcgg ggtcgtcgcg cagctcgccg
      601 atcacggccg cgacgagcct gccggcgctg gccggcgtga ccgcggcgga tccgccgtag
      661 gcgatggagg cggcgcgggc cgcgtctcgt aacgactggg agtgcctggc gtacgaggcg
      721 gcggcgccga ggaggccgtc ggcccggtcg ccgacgacgc cggcggcgcg gacggcgtcg
      781 gacaggtcca tgggtatctg gtacgattcg cttggggtgc cggccaaggc ccaagacgcg
      841 tagcgctcga tcccgccgga gaggacggcc tcgacgtcgg cgccttccag cgagacgaac
      901 agctcttcgc gcggcttgac gatcagcgtg ccgaagaccc ggcccttcgt cgtcatcgag
      961 gccgagtggt cggcgaagcc cggccgcgag acgcggagct cgtgctcgcc ggccggcacg
     1021 aacagctcgc acggcgtata gccgacgtag gctccgtcta tgaacacggc ggagccgggc
     1081 gggtcggctt tgaacgaata gcttgctccg ggacgcctga gcccgggata gagcaggatc
     1141 atgaaggccg cggcgatgag cccgaggccg tacagcccgg ctacgtacag gcgcggggct
     1201 atgccgagga ccggcctgag ccgcacttcg gcgtcgtcta tatcctgttg ggttatc
//
LOCUS       DAOPHV010000766         1256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_161869_length_1256_cov_3.544546, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000766 DAOPHV010000000
VERSION     DAOPHV010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161869_length_1256_cov_3.544546"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..163
                     /locus_tag="PLQ29_14165"
     CDS             14..163
                     /locus_tag="PLQ29_14165"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HPG87837.1"
                     /translation="MAIANVGAAAMGAIGERPEIASQALIFIALAEGLVVFGFITALM
                     ILGKI"
     gene            170..478
                     /locus_tag="PLQ29_14170"
     CDS             170..478
                     /locus_tag="PLQ29_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HPG87838.1"
                     /translation="MKYYVIGDEDTVLGFGMVGVDGRAVSDAAEAEAAFEAATADPEV
                     GILVVTERAAELIRPRVDRYLFSDEFPLIVEIPDRRGRMPGRPTTREMVNKAIGIKLG
                     "
     gene            503..1150
                     /locus_tag="PLQ29_14175"
     CDS             503..1150
                     /locus_tag="PLQ29_14175"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HPG87839.1"
                     /translation="MEQSLNDADAIMAGILADADAEAARLLAEADEYAAQAAARAREQ
                     AATLAAEAAAKAEGQAAAIVAEAEAKAAIERRKRSLLSQERLAAGIIERAVAEVAAMA
                     GAPGYRDVLLAWITEAAIGLSAESAAVNASRADLPLIDDAMLRVAEGAALKATGKATS
                     LRRVDGDPVLGQGVYLTADGGRLAYDNRAATRFERYRADIRRRIYTALGAGKPRS"
BASE COUNT          211 a          395 c          475 g          175 t
ORIGIN      
        1 gatcggcgcc ggcatagcca tagccaacgt cggtgctgcc gcgatgggcg cgatcgggga
       61 gcggcccgag atcgccagcc aggcgctgat cttcatagcg ctggccgagg gcctcgtggt
      121 gttcggcttc atcaccgcgc tgatgatcct gggcaagatc taagggaccg tgaagtacta
      181 cgtgatcggc gacgaggata ccgtactcgg cttcggcatg gtcggcgtcg acggcagggc
      241 cgtctccgac gcggccgagg ccgaggcggc cttcgaggct gccaccgccg acccggaggt
      301 cggcatactc gtcgttaccg agcgcgccgc cgagcttata cggccgcgcg tcgatcgcta
      361 cctgttctcc gacgagttcc cgctgatagt cgagataccc gaccggcgcg gccgcatgcc
      421 cggccggccg acgacgcgcg agatggtgaa caaggccatc gggatcaagc tgggctaggc
      481 gccggttatt aaggatgaag agatggaaca gagcttgaac gacgcggacg cgatcatggc
      541 cgggatcctg gccgacgccg acgccgaggc cgcgcgcctg ctcgcggagg ccgacgagta
      601 cgccgctcag gccgccgcga gggctcgcga gcaggcggcg acgttagccg ccgaggcagc
      661 ggccaaggcc gagggtcagg cggcggccat agtcgccgag gcggaggcca aggccgcgat
      721 agagcggcgc aagcgctctc tcctgtcgca ggagcgcctc gccgccggta tcatcgagcg
      781 ggccgtagcc gaggtcgccg cgatggccgg cgcgccgggc taccgggacg tgttattggc
      841 ttggataacg gaagcggcga tagggctgtc ggccgagtcg gcggcggtca acgcttcaag
      901 ggcggacttg ccgttgatag acgacgcgat gttgcgggtg gccgagggcg cggcgctcaa
      961 ggcgaccggc aaggcgacga gcctgcgcag ggtcgacggc gaccctgtcc tgggccaggg
     1021 cgtctacctg acggccgacg gcggccggct ggcctacgat aatcgggcgg ccacgcgctt
     1081 cgagcgctac cgggccgata ttcgcaggcg tatctatacg gcgctcggcg ccgggaagcc
     1141 gcgctcctag ggcggcgacg aggatggcat gaacgaacgg atcataggac aggtgcgcag
     1201 ggtcaacggg ccggtcatcg aggccatggg catcggcgac gcccgtatgc tcgagc
//
LOCUS       DAOPHV010000767         1254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_162259_length_1254_cov_3.266055, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000767 DAOPHV010000000
VERSION     DAOPHV010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162259_length_1254_cov_3.266055"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PLQ29_14180"
     CDS             complement(<1..464)
                     /locus_tag="PLQ29_14180"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPG87840.1"
                     /translation="MRRTRAPSTLWLYVAKEFLLSFIVSFLFFFIIFFINQLLLMAED
                     ILSKKAPVVDVIKLVVYATPAIIAMSFPFGSLVGALMAAGRLSSDNELLVMRASGVSR
                     PAMMTPFVALGVVFSLISFVTNDYFLPLGTINYGKLYRKLITTAPALELKPYS"
     gene            complement(466..906)
                     /gene="dut"
                     /locus_tag="PLQ29_14185"
     CDS             complement(466..906)
                     /gene="dut"
                     /locus_tag="PLQ29_14185"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPG87841.1"
                     /translation="MDEPVVFLNLMPGAGAPEYRSSGAAGADLRARLDAPVELRPGAT
                     AVIPTGVRIQLPSGYEAQVRSRSGLAAKHGVSCLNAPGTIDSDYRGEIGVILHNHGIE
                     PISIADGDRIAQLVVAPVSRARFVEVDSLSDTDRGAGGFGSTGR"
     gene            complement(899..>1254)
                     /locus_tag="PLQ29_14190"
     CDS             complement(899..>1254)
                     /locus_tag="PLQ29_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87842.1"
                     /translation="SLHREGLTEIEVDEALSRMRGMLELASEDPEFRMKRLARQYMYD
                     GGAETVDATMARLDDGGAVDPVSVNRIIMDRLDPDGESVLLYGKIGAKAAMAGASAFG
                     AEFDEAHSSGGGGHG"
BASE COUNT          237 a          390 c          415 g          212 t
ORIGIN      
        1 gaatacggct tgagttcgag agccggggcc gtcgtgatga gcttgcggta cagcttgccg
       61 tagttgatgg taccgagggg taggaagtag tcgttcgtga cgaaggatat gagcgagaag
      121 acgaccccga gcgcgacgaa aggggtcatc atagcggggc gcgagacgcc ggaggcgcgc
      181 atgaccagga gctcgttatc gctcgacagg cgtccggcgg ccatgagcgc cccgaccagg
      241 gatccgaacg ggaaggacat cgcgatgata gccggcgtgg cgtagacgac gagcttgata
      301 acgtcgacga cgggggcttt cttggacagg atatcctccg ccatcaggag gagctggttt
      361 ataaaaaaga tgatgaagaa gaacaggaac gagacgatga acgacaggag gaactccttg
      421 gccacgtaga gccagagggt cgacggcgcc cgcgtccttc tcatgttagc gcccggtcga
      481 gccgaagccg ccggccccgc gatcggtatc cgaaagcgag tcgacctcga cgaagcgcgc
      541 ccgcgacacc ggcgccacga cgagctgcgc tatccggtcg ccatcggcga tcgaaatcgg
      601 ctcgatgcca tggttgtgga gtatcacgcc tatctcgccc ctataatcgg agtctatcgt
      661 gccgggagcg ttgagacagg agacgccgtg cttggcggcc agtcccgagc gggaccgcac
      721 ctgggcctcg tagcccgaag gtagctggat tctgaccccg gtcgggataa cggccgtggc
      781 gccgggtcgt agctcgacgg gcgcgtcgag tctggcgcgc aggtcggctc cagcggctcc
      841 ggagctccgg tactcgggtg cgccagcccc aggcatgagg ttcaggaata ctaccggttc
      901 atccatggcc gccccctccc gaggaatggg cctcgtcgaa ctcagcgccg aacgccgacg
      961 cgccggccat ggcggccttg gcgcctatct ttccatacag cagcacgctc tcgccgtcgg
     1021 ggtcgagcct gtccatgatg attcggttca ccgataccgg gtcgacagcg cctccgtcat
     1081 cgagcctggc catcgtcgcg tcgaccgtct ccgcgccacc gtcgtacatg tactgcctgg
     1141 ccaggcgctt catcctgaat tccgggtcct ccgaggccaa ttcgagcatg ccgcgcatcc
     1201 tcgacagcgc ctcgtcgact tcgatctcag ttaggccctc gcggtggagc gacc
//
LOCUS       DAOPHV010000768         1247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_163701_length_1247_cov_2.930369, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000768 DAOPHV010000000
VERSION     DAOPHV010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163701_length_1247_cov_2.930369"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="PLQ29_14195"
     CDS             <1..1097
                     /locus_tag="PLQ29_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87843.1"
                     /translation="GSSKFSFGLPDGSVEIDLIAYPDDRFGEAREGAEDTARRLSGRG
                     SFAAFDYQGRGAALGELSFGSGQSAMRGYGLFVNDVAPPADSGGEPYDLVVLAYAPAD
                     GYDGYADFVASAIDGFSIDYGRRAAPGPIGVASRAAVGAARPRPVSLRFGEAEIRTEW
                     NPAEAALSQQLVEREYRVLTAYGRSPELIDAAIARFYRMAFRDAAPSLDRLALELSAA
                     WESGAWAGKKPKSAGAAGAGPAATAEPRAYAEALLAWVQGFTYERDPDGSDVVNPISA
                     AFERRGDCDSRALVMAILLRRENIRSIMMVSLEHEHALAAVDAPGPGARFPFDDGAWL
                     VAETTARVGIGMIDSTQADPSAWIGVAFPF"
BASE COUNT          186 a          439 c          433 g          189 t
ORIGIN      
        1 acggatcgag caaattctcg ttcgggctgc cggacggttc ggtggagatc gaccttatcg
       61 cctaccccga cgaccgcttc ggggaggccc gcgaaggcgc cgaggatacc gccaggcgcc
      121 tgtcgggtcg cgggtcgttc gcggccttcg actaccaggg ccgcggcgcg gcgctcggcg
      181 agctgtcctt cggctcgggc cagtcggcga tgcgaggcta cggcctcttc gtgaacgacg
      241 tcgcgccgcc cgccgattcc gggggcgagc cgtacgacct cgtcgtgctg gcctacgcgc
      301 cggccgacgg ctacgacggc tacgccgact tcgtcgcgtc tgcgatcgac ggcttctcga
      361 tcgactacgg ccgcagggcc gccccggggc cgatcggcgt cgcctcccgc gcggccgtcg
      421 gcgcggcgcg gccgaggccg gtatcgctgc gcttcggcga ggcggagata cggaccgagt
      481 ggaacccggc cgaggccgcg ctgtcgcagc agctggtcga gcgcgagtac cgcgtgctga
      541 ccgcctacgg ccggtcgccc gaactgatag acgccgcgat agccaggttc tacaggatgg
      601 cgttccgcga cgcggcgccg tcgctcgacc gcctggccct cgagctgtcg gcagcgtggg
      661 aatccggcgc ctgggccgga aaaaagccca agtcggccgg ggcagccggc gccggtccgg
      721 ccgcgaccgc cgagcctcgc gcctacgcgg aggcgctgct cgcctgggtg cagggcttta
      781 cctacgagcg cgaccccgac ggctccgacg tggtcaaccc gatatcggcc gccttcgagc
      841 gccgcggcga ctgcgattcc agggccctcg tcatggccat actcctgcgc cgcgagaaca
      901 tacgctccat catgatggta tcgctcgagc acgagcacgc cctcgccgcc gtcgacgcgc
      961 ccggccccgg ggcgcgcttc ccgttcgacg acggggcctg gctggtcgcc gagacgaccg
     1021 ccagggtcgg catcggcatg atagactcga cccaggccga cccgtcggcg tggataggcg
     1081 tagcctttcc gttctgatgg ctgatcgcgg gacaaacagg cgtattatgt catagtgcgg
     1141 cgttgacacc tgaacgacgc cgcaactata cttccggcag cccgaacatg gtcgcggtcg
     1201 cgaccggagg accgttatgg aaccgataag cgctcgttat aaaaata
//
LOCUS       DAOPHV010000769         1241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_165022_length_1241_cov_2.946037, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000769 DAOPHV010000000
VERSION     DAOPHV010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165022_length_1241_cov_2.946037"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="PLQ29_14200"
     CDS             complement(<1..1228)
                     /locus_tag="PLQ29_14200"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HPG87844.1"
                     /translation="MAGGGDVPLSVGANEIAIVVTAEDGATVRTYTLDVTRYGRESTN
                     LASVSLSGISTLSFDPVDDQYELTAPYALTSTVITAAAEYPGSTIRLDTDQDGTYESD
                     FVSGVTAITLGEPGETVSFEFEVTAEYSGATGYYSFDITRAAESASNVAALETLYLRY
                     HADPIATQHPDTLTYSATVPSFVSEVTVDAVASDGGSVVGDGDRALELGANELQLTVT
                     AADGITTAVYTINVTRLAPPTLSITAPTAGAVVDLEASTNLTVSGAFDDPNGEVQGVT
                     VLIGSNQVEATVEGATYSASADLSGMTNGTKNILVFATNDGYYGGDTPIALATSSITV
                     TGGAEGYDLSFSIALPPGLASLEAGYLSIALLDPDTGATAALTLDIPCSTITFPYAYG
                     FEGVASGSYGLMIMVTD"
BASE COUNT          219 a          394 c          397 g          231 t
ORIGIN      
        1 cgtcggtcac catgatcatc aagccatagc tgccggaagc gacgccctcg aagccatagg
       61 cgtaggggaa cgttatcgtc gagcaaggga tatccagcgt cagcgccgcg gtcgcccccg
      121 tatccggatc gagcagggcg atggacaggt accccgcctc gaggctggcg agccccggcg
      181 gcaaggctat cgagaacgac aggtcgtagc cctcggcccc gcccgtcacc gttatggacg
      241 aggtggctaa cgctatcgga gtgtcgccac catagtatcc gtcattggta gcgaagacca
      301 agatattttt ggtaccgttc gtcatcccgc tcaggtccgc gctagcgctg tacgtggctc
      361 cctcaacggt cgcctccacc tggttcgacc cgatgagtac ggtaacgcct tggacctcgc
      421 cgttcgggtc atcgaaggcg ccagagacgg tcaggttcgt cgaggcctcc aggtcgacga
      481 cggcgccagc cgtcggagcc gttatggata gggtcggtgg agcgagcctc gtcacattga
      541 tcgtgtatac cgccgtagtt atcccgtcgg cggcggtcac cgtgagctgc aactcgttgg
      601 ctcccagctc tagggcgcgg tcgccgtcgc cgacgacgga gccgccgtcg ctggctaccg
      661 cgtccaccgt cacttcggag acgaagctcg gcacggtggc ggaatacgtc agggtgtcgg
      721 gatgctgcgt tgcgatagga tcggcatggt atctaaggta caaggtttcc agggcggcca
      781 cattcgaggc tgactccgcg gctcgggtta tatcgaaact gtaatacccg gtcgcacccg
      841 aatactcggc ggtcacctcg aactcgaagg atacggtctc gccgggctct ccgagcgtaa
      901 tggcagtcac gccgctaacg aagtcgctct cataggttcc atcttgatcc gtgtccagcc
      961 gtatcgtgga gccgggatac tcggccgccg ccgtgatgac ggtgctggta agggcgtacg
     1021 gggccgtcaa ctcatactga tcgtccacag gatcgaaact cagcgtcgag atgccgctca
     1081 gggacaccga ggccaggttc gtgctctccc ggccgtagcg ggtcacgtcg agggtatagg
     1141 tccggacggt ggcgccgtcc tccgcggtca cgacgatcgc tatctcgttg gcgccgacgc
     1201 tcagcggcac gtctccgccg ccggccaccg ccgcgccatc g
//
LOCUS       DAOPHV010000770         1240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_165199_length_1240_cov_3.459072, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000770 DAOPHV010000000
VERSION     DAOPHV010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165199_length_1240_cov_3.459072"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLQ29_14205"
     CDS             complement(<1..196)
                     /locus_tag="PLQ29_14205"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPG87845.1"
                     /translation="MFSLAAFSLGGAMGVSLVGASGAVYAVLLAFAVINPRARIFIWG
                     VVPVPAPILVAGYTVIELWSQ"
     gene            complement(214..516)
                     /locus_tag="PLQ29_14210"
     CDS             complement(214..516)
                     /locus_tag="PLQ29_14210"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPG87846.1"
                     /translation="MTFIQRIRKPFRFTNGNVTLVLIGVNVLVYALTSVAREAYVYLA
                     MIPAAVLQLGWVWQPFTYMFVHANLRHLLFNMLGLFFFGSSVERSLARGSSCSSTC"
     gene            652..840
                     /locus_tag="PLQ29_14215"
     CDS             652..840
                     /locus_tag="PLQ29_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87847.1"
                     /translation="MPTNPPRAPNCLQCVHFRVTWDPAFPRSCDVFDIKTRRMPSAEV
                     YAATGLHCPSFELKPGLK"
     gene            complement(841..>1240)
                     /locus_tag="PLQ29_14220"
     CDS             complement(841..>1240)
                     /locus_tag="PLQ29_14220"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPG87848.1"
                     /translation="LDPSGGVEYSSADHSGALYLGADRVRAAPLRPKGQGDYMGPPLG
                     RRGVDAPYTSMRFKLRQGDVILIYTDGFGGAENVDGAPFGEAGMLEALTSAPEGDAAQ
                     MLDFIVREWRFHVSGARVSDDATAVLLKRL"
BASE COUNT          224 a          418 c          424 g          174 t
ORIGIN      
        1 gctgggacca tagctcgatg accgtgtagc cggcgacgag tatgggggcg ggcaccggta
       61 ccacgcccca gatgaagatc ctggcccgcg ggttgatcac cgcgaacgcg agcagcaccg
      121 cgtacaccgc gcccgacgcg ccgaccaggc tgacgcccat cgcgccgccg agcgagaagg
      181 cggccagcga gaacagcccc gcgagcgcgc cggtcagcag gtagaagaac aggaactccc
      241 tcgagccagc gatcgctcga ccgagctgcc gaagaagaac aggccgagca tgttgaacag
      301 gaggtgcctg agattggcgt ggacgaacat gtaggtgaag ggctgccaga cccagccgag
      361 ctggagcacg gcggccggga tcatggccag gtagacgtag gcctcgcgcg cgacgctggt
      421 cagcgcgtag acgagcacgt tgacgccgat cagcacgagc gtgacgttgc cgttggtgaa
      481 acggaagggc ttcctgattc tctgtatgaa agtcatatcg ttaatgtacg caaaaaacgc
      541 cgcctctgtg tagtgtcacg aaccttatgc atggacgggg cggcctcgag gctcggaacg
      601 gcgacgacgc gcgcccttgc ccggcggccg ccgggccgct acactatcgt catgccgacc
      661 aacccgccgc gggcgccgaa ctgcctgcaa tgcgtccact tccgcgtgac ctgggacccg
      721 gccttccccc gctcctgcga cgtcttcgac atcaagacgc gccggatgcc gagcgcggag
      781 gtctacgcgg cgaccgggct ccactgcccg tcattcgagc tgaagcccgg cctcaagtag
      841 ctacagccgc ttgagcagca ccgcggtcgc gtcgtcggag accctggccc cgctgacgtg
      901 gaaacgccat tccctgacga tgaagtcgag catctgggcc gcgtcgccct cgggggccga
      961 ggtcagggcc tcgagcatgc ccgcctcgcc gaacggggcg ccgtcgacgt tctcggcgcc
     1021 gccgaagccg tccgtgtaga tcaggatgac gtcgccctgc cgtagcttga agcgcatcga
     1081 cgtatacgga gcgtcgacgc cgcgccggcc gaggggcggg cccatgtagt cgccctggcc
     1141 cttgggccgt agcggcgcgg cgcgaaccct gtcggccccg aggtacagcg ctcccgagtg
     1201 gtcggcgctc gagtactcga cgccgccgga cgggtcgagg
//
LOCUS       DAOPHV010000771         1239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_165389_length_1239_cov_4.486486, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000771 DAOPHV010000000
VERSION     DAOPHV010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165389_length_1239_cov_4.486486"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PLQ29_14225"
     CDS             <1..845
                     /locus_tag="PLQ29_14225"
                     /inference="COORDINATES: protein motif:HMM:NF037014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HPG87849.1"
                     /translation="HSWVMAFESELASFEAYADMYPDGTTLLIDTFDTLGSGLPNAIK
                     VGNRLAGRGKKLGVRLDSGDIDYLSRKVREGLDEAGLDDAFIVVSNELDEEIIETLVA
                     AGAPVDVWGVGTHLVTGGDEASFTGVYKLAAFERGGVWSPTMKFSDNPDKSTNPGVKD
                     LYRVYDESGAAVADVMTLAGETIEPGKMVTFHHPSLDTRRVTFEPCGEIRPMLGKVME
                     GGKRLAPAPTMQELRARRAASMRSFDHTFLRFLNPHIYKVSISGRLRDLKLEFVERYR
                     GSKG"
     gene            complement(852..>1239)
                     /locus_tag="PLQ29_14230"
     CDS             complement(852..>1239)
                     /locus_tag="PLQ29_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87850.1"
                     /translation="RDESWLLPPRTTLGALVASGLDRRADLALGPAAFASAEAATPFW
                     LARASSTPTADGATGDSRPTRVGDGYLYPDETVVTPPPGEYAPTGRRVVAAAGSPPER
                     RVAVALALALLYGLKAWLGLRRRRAD"
BASE COUNT          203 a          431 c          424 g          181 t
ORIGIN      
        1 cccatagctg ggtgatggcc ttcgagagcg agctcgcctc gttcgaggct tacgccgaca
       61 tgtacccgga cggcaccacc ctgttgatcg acaccttcga cacgctcggc tccggcctgc
      121 cgaacgcgat caaggtcggc aatcgcctgg cagggcgagg caagaagcta ggcgtcaggc
      181 tcgactccgg cgatatagac tacctgtcgc gcaaggtacg cgaggggctt gacgaggctg
      241 gcctcgatga cgccttcatc gtcgtatcca acgagctcga cgaggagatc atcgagacgc
      301 tcgtagccgc cggcgcgccg gtcgacgtct ggggcgtcgg tacccacctg gtcaccggag
      361 gcgacgaggc atccttcacc ggcgtctaca agctggccgc ctttgagcgg ggcggcgtat
      421 ggtcgccgac gatgaagttc tcggacaatc ccgacaagtc gaccaatccc ggcgtcaagg
      481 acctgtaccg cgtctacgac gagtctggcg ccgccgtggc cgacgtgatg acgctcgccg
      541 gcgagacgat agagcccggg aagatggtca ccttccatca tccgtcgctc gacaccaggc
      601 gcgttacctt cgagccgtgc ggcgagatac ggcccatgct cggcaaggtc atggagggtg
      661 gcaagcgcct cgcgccagcc ccgacgatgc aggagctacg cgccaggcgc gccgcgtcga
      721 tgcggtcgtt cgaccacacc ttcctgcgct tcctgaaccc gcacatctat aaagtatcca
      781 tcagcgggcg gctacgggac ctcaagctcg aattcgtcga gcgctaccga ggctccaagg
      841 gctagccgct ctcagtcggc ccggcgtcgc ctcaggccga gccaggcctt gaggccgtag
      901 agcagggcga gcgccagcgc gacggcgacc ctgcgttcgg gcggcgagcc cgcagccgcg
      961 acgaccctgc gtcccgtggg cgcgtactcg cccggcggcg gcgtgacgac ggtctcgtct
     1021 gggtacaggt atccgtcgcc gacgcgcgtc ggcctcgagt cgcctgtcgc cccgtcggcc
     1081 gtcggcgtcg acgaggcccg ggcgagccag aagggcgtcg ccgcctcggc cgaagcgaag
     1141 gccgccggcc cgagcgccag gtcggcccgc cggtccagcc cggaggcgac gagggcgccg
     1201 agggtggtcc tcggcggcag gagccacgac tcgtcccgg
//
LOCUS       DAOPHV010000772         1230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_167293_length_1230_cov_4.053617, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000772 DAOPHV010000000
VERSION     DAOPHV010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167293_length_1230_cov_4.053617"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1230)
                     /locus_tag="PLQ29_14235"
     CDS             complement(<1..>1230)
                     /locus_tag="PLQ29_14235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPG87851.1"
                     /translation="MFSPKARTMFGVEDGEDRIGCLVTDFMAPEDRPRAAVNIGRMLA
                     GEPLGAEQYTGLRADGSTFDMEINGAIIRDGDGRPKRILFVVRDITARNRLVGKYRIL
                     FDQSPLGIGLFDFCTERFVDANPALQRMTGYGLDELRSMTFLDVTAPEEIERSRELVS
                     LLGERGTLGPYEKLYRRKDGALMPVSINAVKFDDYDGRPVVWGIIEDITVRRAAERRV
                     ESLLAEKDLILKEVHHRLKNNFSVIRGLLGLQADSMTDPSAAEAFLATEARVQSMALL
                     YEKLYESSRFSEASMADYLPSLVREVVANYAHAADVRVETDAADVRLDVKRLQALGIL
                     VNELVSNSMKHAFKGRASGLVFLRAGLEGGRVTVTVGDDGPGMPEPPPSDASGGGFGL
                     TLVSLLATQLRADIARE"
BASE COUNT          205 a          416 c          402 g          207 t
ORIGIN      
        1 actcgcgagc aatatcggct cggagttgcg tagccagtag ggacacgagg gtcaacccga
       61 aaccgccgcc ggacgcgtcg ctcggcggcg gctcgggcat gccaggcccg tcgtcgccca
      121 ccgtcaccgt cacccggccg ccctcgagcc cggcgcgcag gaacacgagc ccgctcgctc
      181 gccccttaaa ggcgtgcttc atgctgttcg acacgagctc gttgaccagg atgccgaggg
      241 cctggagccg cttcacgtcc aggcgcacgt ccgccgcgtc ggtctccacg cgcacgtccg
      301 cggcatgggc gtagttggcg accacctcgc ggacgagcga gggcaggtag tcggccatgg
      361 acgcctccga gaagcgcgag ctctcgtaca gcttctcgta caggagcgcc atgctctgca
      421 cacgggcctc ggtggcaagg aaggcctcgg cggcggacgg gtcggtcatc gaatcggcct
      481 gcagaccgag tagcccgcgt atcacggaga aattgttctt gaggcggtgg tgcacttctt
      541 tgaggatcag gtccttctcg gcgagcagcg actcgacgcg gcgctcggcg gcccggcgca
      601 cggtgatgtc ctcgatgatg ccccagacga ccggccggcc gtcgtaatcg tcgaacttga
      661 cggcgttgat ggagaccggc atcagtgccc cgtctttccg tcgatacagc ttctcgtagg
      721 gcccgagggt cccgcgctcc ccgaggaggc tcacgagttc ccgggatcgc tcgatttcct
      781 ccggtgcggt tacgtccagg aaggtcatcg agcgcagctc gtcgagcccg tagccggtca
      841 tacgttgcag ggccggattc gcgtcgacga aacgctcggt gcagaaatcg aacaacccta
      901 tcccgagcgg cgactggtcg aacagtatcc ggtacttgcc gaccaggcgg ttcctggcgg
      961 taatgtcgcg gacgacgaac aggatacgct tggggcgccc gtctccatcg cggatgatcg
     1021 ccccgtttat ctccatatcg aaggtcgagc cgtcggcgcg gaggccggta tactgctcgg
     1081 cgccgagcgg ctccccggcc agcatgcggc cgatattgac cgccgcccgc ggcctatcct
     1141 ccggcgccat gaagtccgtg accaggcagc caatgcgatc ctcgccgtcc tcgacgccga
     1201 acatcgtcct ggccttgggc gagaacatga
//
LOCUS       DAOPHV010000773         1230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_167295_length_1230_cov_3.980426, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000773 DAOPHV010000000
VERSION     DAOPHV010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167295_length_1230_cov_3.980426"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..>1230)
                     /locus_tag="PLQ29_14240"
     CDS             complement(120..>1230)
                     /locus_tag="PLQ29_14240"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPG87852.1"
                     /translation="AAKTLLTGSKHGVGAYSESKGLKVVRDAVAAFIERRDGIAADPD
                     AIYLTDGASKGVQAALRLLLSGPDDGIMIPIPQYPLYSATITMYEGRQVPYYLDEAHD
                     WALSRDMLEEAIAKAEAAGVRTKAIVVINPGNPTGSVLRPDNVKMVIRFAKDHGLAIL
                     ADEVYQDNVYRDGDRFESFAKAMAELGETGVSLFSFHSTSKGYLGECGQRGGYMEVRN
                     VGPEVMAQVLKMQSVSLCANLPGQVAMYSLVAPPAVGGPSRPEYDRERSAILGELRER
                     ARLLADGLNAIPGFHCNAVAGAMYAFPRLELPAGRTDEDWCMALLESKGICVVPGSGF
                     GQLPGTAHFRTTILPPRDQLAEVVAAIASFNAEYR"
BASE COUNT          169 a          384 c          463 g          214 t
ORIGIN      
        1 gagaactgcc tggcgaggga gccgaagtcg gcgccctgct tggccttgcg tacgagctcc
       61 tgcgccaggg cgcggtcgct gacgagtatg tggctggctc tccattccat gatcgtctcc
      121 tatcggtact cggcgttgaa cgacgctatc gccgccacga cctcggcgag ctggtctcgg
      181 ggcggcagta tggtggtgcg gaagtgggcg gtaccgggta gctgtccgaa gcccgagccc
      241 ggcacgacgc agatgccctt gctctcgagc agggccatgc accagtcctc gtcggtcctg
      301 cccgccggca gctcgaggcg ggggaaggcg tacatggcgc cggcgacggc gttgcagtgg
      361 aagccgggga tggcgttgag gccgtcggcg aggagcctcg cgcgctcgcg cagctcgccg
      421 agaatggccg agcgctcgcg gtcgtactcg gggcggctcg gtccgccgac cgcgggcggg
      481 gcgacgaggc tgtacatggc cacctggccg gggaggttgg cgcagaggct gacggactgc
      541 atcttgagca cctgggccat cacctcgggc ccgacgttgc ggacctccat gtagccgcct
      601 ctctggccgc actcgcctaa gtatcccttg ctggtcgagt ggaagctgaa cagcgacacg
      661 ccggtctcgc cgagttcggc catcgccttg gcgaacgact cgaagcggtc gccgtcgcgg
      721 tagacgttgt cctggtagac ctcgtcggcc aggatggcca ggccgtggtc cttggcgaag
      781 cggatgacca tcttgacgtt gtccggcctc aggaccgagc cggtcgggtt cccggggttg
      841 atgacgacga tggccttggt cctgacgccg gcggcctcgg ccttggcgat cgcctcctcg
      901 agcatgtcgc gggacagggc ccagtcgtgc gcctcgtcga ggtagtaggg tacctggcgg
      961 ccctcgtaca tggtgatggt agcggagtac agcgggtact ggggtatcgg gatcatgatg
     1021 ccgtcgtcgg ggccggatag gagcaggcgc agcgccgcct ggacgccctt ggaggcgccg
     1081 tcggtcaggt agatcgcgtc ggggtcggcg gctatgccgt cgcggcgctc gatgaaggcg
     1141 gcgacggcgt cgcggacgac cttgagcccc ttgctctcgg agtaggcccc gacgccgtgc
     1201 ttggagccgg ttagcagcgt cttggcggcc
//
LOCUS       DAOPHV010000774         1222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_169049_length_1222_cov_3.716367, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000774 DAOPHV010000000
VERSION     DAOPHV010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169049_length_1222_cov_3.716367"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PLQ29_14245"
     CDS             complement(<1..986)
                     /locus_tag="PLQ29_14245"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPG87853.1"
                     /translation="MSTDRTPLVDLALRDDDVLYDKLSIIIDSLDNLHQLDKTAFLKQ
                     VFEAAFLLIPEAQKGSLYELREGRYRPIFSRGYDEALLSRLTFGESEAFVDFTMDDDG
                     RSSAYETLIIKRNSSAFNPETLELFRRLGTDGGFRTLYAPIRAEGKTFGLISLEKFDD
                     GDFGRFSRKILQYYAWLISEFYAQRLRQEQETGHYREVIGSLISAIEVNDSYTNGHGR
                     RVAAISGRLGMFLGLEPPELRDLHTAALLHDIGKIGVPSEILRKPGGLDEREYSIVKR
                     HPVDSAKILGGISGFARVVELALRHHERYDGTGYPEGLSGDQLPLSAYVLQLA"
BASE COUNT          219 a          345 c          404 g          254 t
ORIGIN      
        1 gccagctgga ggacgtaggc cgacagcggc agctggtcgc cagagagtcc ctcggggtag
       61 cctgtgccgt cgtagcgctc gtggtggcgc agagccagct cgacgacccg ggcgaagccg
      121 ctgatgccgc cgagtatctt ggcggagtct accggatgcc gcttgacgat gctgtattcc
      181 cgctcgtcga gcccgcctgg cttgcgcagt atctccgagg gcacgcctat cttgccgatg
      241 tcgtggagca gggcggccgt gtgcaggtcg cgcaactccg gcggctcgag gcccaggaac
      301 atgccgaggc ggccgctgat cgcggcgacg cgcctgccat ggccattggt gtagctgtcg
      361 ttcacctcga tggcggagat cagcgagcct atcacctcgc ggtagtggcc ggtctcctgc
      421 tcctgcctga ggcgttgagc gtagaattcc gatattagcc acgcgtagta ctggagtatc
      481 ttgcgcgaaa agcggccgaa atcaccgtcg tcgaatttct ccaggctgat gagcccgaag
      541 gtcttgccct cggcccgtat cggcgcgtac agcgtgcgga agcccccgtc ggtgccaagc
      601 cgcctgaaca gctcgagcgt ttcgggattg aaggccgagg aattgcgctt gatgatcaag
      661 gtctcatagg cgctggatcg gccgtcgtcg tccatggtga aatcgacgaa ggcctcgctc
      721 tcgccgaacg tcagcctcga caggagggct tcgtcgtagc cgcgcgagaa gatcgggcgg
      781 tacctgcctt cccgcagctc gtagagggag cccttctgcg cctcgggtat cagcaggaat
      841 gcggcctcga agacctgctt gaggaacgcg gtcttgtcga gctggtgcag gttgtcgagc
      901 gagtcgatga tgatggagag tttgtcgtag agaacatcgt cgtcgcgtag ggctagatcg
      961 accagcggcg tcctgtccgt gctcacgtgg cgcggcctcc tcaggcgatt gctcgagatg
     1021 gttactgttc gttgggttac ctagtcgacg taccgcgacc cgctcggagc cttcgaatag
     1081 tcgaagctat gtatagcaaa gaacgtgatg tctgtatagc gggcctttgg actctttcga
     1141 cagaatcgat atatgcagac tggcttcgcg atggtggata cgatagaacg gccctgtgtc
     1201 gtgaaagtgt ttcattaaga aa
//
LOCUS       DAOPHV010000775         1219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_169699_length_1219_cov_3.969072, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000775 DAOPHV010000000
VERSION     DAOPHV010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169699_length_1219_cov_3.969072"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PLQ29_14250"
     CDS             complement(<1..563)
                     /locus_tag="PLQ29_14250"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPG87854.1"
                     /translation="MAQFLQQMVNGLSIGSVYALMAVGYSLVYSIMNFSNFAHGGVIM
                     FGAYFGFFAMTLLKLPFVASFALAFVGTSVLAVVIERVAYKPLRERSAPFLYFIISAM
                     GASIFLENIIIATIGPTFRTYPDVFGRSSISLGSVSIGRLDLIMFVISAVCLTGLVLF
                     IDKTKMGRAIQASAYNMKAAALMGVNT"
     gene            complement(709..>1219)
                     /locus_tag="PLQ29_14255"
     CDS             complement(709..>1219)
                     /locus_tag="PLQ29_14255"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPG87855.1"
                     /translation="FRPQLSKIKAQNPDIIFMPYFFKEVALSANQARELGITQVLMGG
                     DGWPSDQLISMGGAAVEGCYFVNHLDYEDPDVADYKARYLKKYGIETELNGYLVHDAV
                     VLLVDALKRAGEPSGEAIAKALESADVKGITGRIKISPQTHNPEGKDAAIIKIVGGKY
                     IFQQKYAAE"
BASE COUNT          229 a          353 c          413 g          224 t
ORIGIN      
        1 tcggtgttca cgcccatcag ggcggcggcc ttcatgttgt aagccgacgc ctgtatggcg
       61 cggcccatct tggtcttgtc gatgaagagt acgaggccgg tcaggcagac ggccgagatg
      121 acgaacatga tcaggtcgag ccgccctatg gacaccgagc cgagcgatat ggacgaacgg
      181 ccgaaaacgt ccgggtaggt gcggaaggtc gggcctatcg tcgctatgat gatattctcg
      241 aggaagatcg aggcgcccat cgccgagatg ataaagtaca ggaagggcgc gctacgctcg
      301 cgcaacggct tgtaggcgac gcgctcgatg acgaccgcca ggactgacgt accgacgaag
      361 gccagagcga acgacgcgac gaagggcaac ttgagtagcg tcatcgcgaa gaagccgaag
      421 taagccccga acatgatgac gccgccgtgc gcgaagttcg agaaattcat gatggagtac
      481 acgagcgagt agccgacggc catcagcgcg tagacgctac cgatggagag gccgttgacc
      541 atctgctgca ggaactgagc cataagcgct ccgggtttca ggcgacgggg gccgccgctg
      601 gggatagggc ggccgaggcc ccccgattcg tcaaggaggg agcccccgcc tgagcgggcg
      661 ctccctccgg tctgcattca gtgattgttc agaaatgctc accccggatc actccgcggc
      721 gtatttctgc tggaagatgt acttgccgcc gacgatcttg atgatggcgg cgtccttgcc
      781 ctcggggtta tgggtctgcg ggctgatctt gatccgcccg gtgatgccct tgacgtcggc
      841 gctctcgagg gccttggcta tcgcctcgcc cgagggctcg ccggcgcgct tcagggcgtc
      901 gacgaggagc accaccgcgt cgtggaccag gtagccgttc agctcggtct cgatgccgta
      961 cttcttgagg tagcgggcct tgtagtcggc cacgtccggg tcctcgtagt cgaggtggtt
     1021 gacgaagtag caaccttcga cggctgcgcc gcccatgctg atcagctggt cgctcggcca
     1081 gccgtcgccg cccatcagta cctgtgtgat gccgagctcg cgcgcctggt tggcggacag
     1141 cgcgacctcc ttgaagaagt agggcatgaa gatgatgtca gggttctggg ccttgatctt
     1201 ggacagctgg ggcctgaag
//
LOCUS       DAOPHV010000776         1219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_169749_length_1219_cov_2.764605, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000776 DAOPHV010000000
VERSION     DAOPHV010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169749_length_1219_cov_2.764605"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            378..1085
                     /locus_tag="PLQ29_14260"
     CDS             378..1085
                     /locus_tag="PLQ29_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87856.1"
                     /translation="MNKKSTDFIQQARTLIENAAGTPEIAKALAVYGYTEARFKDGLD
                     LLGRTEKLMMRKNMESGEKIEATADFVKAWREANVMYIKTLKIARIALEDEAKSDAGL
                     LLSGARKQTFSGWSFQASNFYGNLLSRKALVDKMGVYGYNKEALAKEKDVVDQAVKTY
                     GRHAIQSGEAQVATQEKDAKLDELDAWVSALRAVCEIALYDDREQLEKLGPLAPIRRS
                     RPAKKAPKPTPEPAVIA"
BASE COUNT          267 a          412 c          353 g          187 t
ORIGIN      
        1 cctctctcag tcgttcggga acctctctca gtcgttcggg aacctctctc agtcgttcgg
       61 gaacctctct cagccgctcg ggagcctctc tcagtcgctc gggaacctct ctcagccgct
      121 cgggaacctc tctcagtcgc tcgggaacct ctctcagtcg ctcgggaacc tctctcagcc
      181 gctcgggagc ctctcagccg ctcgggaacc tctctcagtc gctcgggaac catacaaggc
      241 aggtcgacga tcaatcctga ttgctcaagc aacgactcgg tcggcaatga agccaagcaa
      301 cgccgacaga gccgatgtat tccaagcaca tcgatgcctg tcgttgacag gtcaataaag
      361 aacagaggag gaatgggatg aacaaaaaga gcacggattt catccagcag gcgcgcaccc
      421 tgatcgagaa cgcagcgggc acgccggaga tagccaaggc gctcgccgtc tacggctaca
      481 cggaggcccg gttcaaggat gggctcgacc tcctcggacg gaccgagaaa ctgatgatgc
      541 ggaagaacat ggagagcggc gagaagatcg aggcgacggc cgacttcgtc aaggcctggc
      601 gcgaggccaa cgtcatgtac atcaagacgc tcaagatagc gcgcatagcg ctcgaggacg
      661 aggccaagag cgacgccggc ctcctcttat ccggcgcccg caagcagacc ttctccggct
      721 ggagcttcca ggccagcaac ttctacggca atctcctgtc gcgcaaggcc ctcgtcgaca
      781 agatgggcgt ctacggctat aacaaggaag cgctcgccaa ggagaaggac gtggtcgacc
      841 aagccgtcaa gacctatggg cgccacgcca tccagtcggg cgaggcccag gtcgccaccc
      901 aggagaagga cgccaagctc gacgaactgg acgcctgggt ctcggccctc cgcgccgtct
      961 gcgagatagc cctctacgac gaccgcgagc agctcgagaa gctcggcccc ctcgccccta
     1021 tccgccgctc ccgcccagcc aagaaggcgc caaaaccgac gcccgaaccc gccgtcatag
     1081 cctgactgcc agccggcgag atgccctaag cctctcgccg gcgtttcgcc cagcgcgact
     1141 cgccggccgc cgtcagactc cgcgctcgcg gaggcggacg tgatacgaat aatggcgaca
     1201 tgatgaacct gatagtacg
//
LOCUS       DAOPHV010000777         1214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_170820_length_1214_cov_3.041415, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000777 DAOPHV010000000
VERSION     DAOPHV010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170820_length_1214_cov_3.041415"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PLQ29_14265"
     CDS             <1..745
                     /locus_tag="PLQ29_14265"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HPG87857.1"
                     /translation="SALGRMHGRPVGIVGAGRTDSGVHAAGQVAHFVTDIARIPANRF
                     EPALNRLMPRDVRIVRAEDAHPDFHARFDARLRRYKYYIRYGDAQLPHLDRYSWRLFR
                     RPDLRRLNRLASCLRGELDCEAFAAAKDPSSSRFRYLHHAAFYAEGDAIVFDIAANAF
                     LWRMVRSIVGTLIELDASGAPETAMRDILESRERGRAGVTAPARGLFLWNVEYYDPPT
                     KVRDVSEGDDVSDGDVRYKGDDVSEGDDE"
     gene            742..>1214
                     /locus_tag="PLQ29_14270"
     CDS             742..>1214
                     /locus_tag="PLQ29_14270"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPG87858.1"
                     /translation="MKPRMSAALFDLDGTLLDSEPAYLESDRAFLAGYGVDYTDELNR
                     GYVGRGSKAMLEDLAARFPDSRLASAPIAELVALKDKAYASYAPGRVRAFPATAALAR
                     ALAARGVALAIASGSSRAVIDLMIGSGSLGALFPVRVSSEEVPRGKPEPDVFIEAA"
BASE COUNT          182 a          449 c          390 g          193 t
ORIGIN      
        1 gtcggcactt ggccgcatgc acggccgccc ggtgggcata gtcggcgccg gccgtaccga
       61 ttcgggcgtg cacgccgcgg gccaggtggc ccatttcgtc accgacatcg cccgcatacc
      121 agccaaccgc ttcgagcctg ccctgaaccg gctcatgccg cgcgacgtcc ggatcgtgcg
      181 cgcggaggac gcccatcccg atttccacgc ccgcttcgac gccaggctca ggcgctacaa
      241 gtactatatc cgctatggcg acgcgcagct gccgcatctc gaccgttact cgtggcggct
      301 cttcaggcgg cccgacctgc gcaggctcaa ccgcctggcc tcctgcctac gcggcgagct
      361 ggactgcgag gcgttcgccg ccgccaaaga cccgagctcc agccgcttcc gctacctgca
      421 ccacgccgcg ttctacgccg agggcgacgc catcgtcttc gacatagccg ccaacgcgtt
      481 cctgtggcgc atggtccgtt cgatagtcgg cacgctgatt gagctggacg cctccggcgc
      541 ccccgaaacg gcgatgcgcg acatcctcga gtcgcgggag cggggcaggg ccggcgtcac
      601 cgccccggct cgcggcttgt tcctctggaa cgtcgaatac tatgacccgc cgaccaaagt
      661 ccgcgacgtc tccgaaggag acgacgtctc cgatggtgac gtccgctaca agggagacga
      721 cgtctccgaa ggagacgacg agtgaaaccc cgtatgtccg ccgcgctgtt cgacctggac
      781 ggtaccctgc tcgattcgga gccggcctac ctggagagcg accgcgcctt cctcgccggc
      841 tacggcgtcg actataccga cgagctgaac cgcggttacg taggccgcgg ctccaaggcc
      901 atgctcgagg acctcgcggc tcggttcccc gatagccggc tggcctccgc gccgatagct
      961 gagctggtcg cgctgaagga caaggcctac gcgtcctacg cgcctggtag ggtgcgcgcc
     1021 ttcccggcca cggccgcgct cgcccgagcc ctggcggccc gcggcgtcgc cctggccata
     1081 gcctccggct cgtcgcgcgc cgtgatagac ctgatgatag gctcgggaag cctcggcgcg
     1141 ctgtttcccg tcagggtctc gtccgaggag gtgccgcgag gcaagcccga gcccgacgtg
     1201 ttcatcgagg cggc
//
LOCUS       DAOPHV010000778         1212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_171236_length_1212_cov_3.644771, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000778 DAOPHV010000000
VERSION     DAOPHV010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171236_length_1212_cov_3.644771"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="PLQ29_14275"
     CDS             complement(<1..1098)
                     /locus_tag="PLQ29_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HPG87859.1"
                     /translation="MNAKPVLALFNSELRAINLGLDSFAANLDREGAKAVQVDWRPPA
                     GGDVKAIAALDAIAAGATVDIEKANAEAVRRILAAEPTVIGIGVAKDVVPGMEPNLIL
                     HAGPPVTWEKMCGPMRGAVIGGLLFEGLAKTPEEAAALAASGKIRFDPCHHHSAVGPM
                     AGVMTASMPVWILENKAFGNKAYCTLNEGLGKVLRYGAYSAEVIDRLAWMRDELAPIL
                     SGALALHGPIDMKSVIAQILQMGDEGHNRNRAGTSIVIRELAPHIVMLDYPREKLSKA
                     LAFLNANDHFFLNLTMPSAKCSVDAAAGIEGSTIITTMARNGTEFGIRLSGLPGRWFT
                     GPSGIVDGLYLPGFGPEHAARGRGQDVPAEPN"
BASE COUNT          177 a          390 c          428 g          217 t
ORIGIN      
        1 gttcggctcg gccggcacat cctggccccg gccgcgggcc gcgtgctctg ggccgaaacc
       61 cggcaggtac agtccatcga cgattccgga cggcccggtg aaccagcggc ccggtaggcc
      121 ggagagcctg atgccgaact cggtaccgtt gcgggccatc gtcgtgatga tcgtcgagcc
      181 ctcgatgccg gcggccgcgt cgacggagca cttggcgctc ggcatcgtca ggttcaggaa
      241 gaaatggtcg ttggcgttca ggaaggcgag cgccttcgac agcttctccc tggggtagtc
      301 gagcatcacg atatgcggcg ctagctcccg gatgacgatg gaggtgccgg cgcgattgcg
      361 gttgtggccc tcgtctccca tctgcaggat ctgggctatc acgctcttca tgtctatcgg
      421 gccgtggagc gccagggccc cggataggat cggagccagc tcgtcgcgca tccaggcgag
      481 gcggtctatg acctcggcgg agtaggcgcc gtagcgcagc accttgccca gaccctcgtt
      541 gagggtgcag taggccttat tgccgaaggc cttgttctcc aggatccaga cgggcatgct
      601 cgcggtcatg acgccggcca tggggccgac cgcactatgg tgatggcatg gatcgaaacg
      661 tatcttgccc gatgcggcga gggccgcggc ttcctcgggc gtcttggcca agccctcgaa
      721 cagaagcccg ccgatgaccg cgccgcgcat cgggccgcac atcttctccc aggtgaccgg
      781 ggggccggcg tgcaggatga ggttcggctc catgcctggc acgacgtcct tggcgacgcc
      841 tatgccgatg acggtcggct cggccgcgag gatgcgtcgg acggcctcgg cgttggcctt
      901 ctcgatgtcg actgtcgcgc cggcggctat cgcgtcgagg gcggctatgg ccttgacgtc
      961 gccgccggcc ggtggccgcc agtcgacctg gactgccttg gcgccctcgc ggtcgaggtt
     1021 ggcagcgaag gagtcgagcc ccaggttgat cgcacgcagt tcggagttga ataaggcgag
     1081 tacgggcttg gcgttcatag ggcgcctcct ttcgcggcga gcttggctat ctcgatggcg
     1141 acgagggccg cccggtagtt cgacggcatc acgacgacgc cgcgaggcct ccccgacgtc
     1201 agcgcgttcc cc
//
LOCUS       DAOPHV010000779         1208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_172150_length_1208_cov_3.666088, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000779 DAOPHV010000000
VERSION     DAOPHV010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172150_length_1208_cov_3.666088"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PLQ29_14280"
     CDS             <1..691
                     /locus_tag="PLQ29_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPG87860.1"
                     /translation="WLASLKVTFTYTFWEIVITLVGGILLAALFNREGRVFAAMRSIV
                     FMPKYIAISTSAIVFMWILNDQYGFLNYVLGAFGVGPVAWLNSERTALTSVLILTGWR
                     VVGYGMMIYLSAMKGISRDYYEAAELDGAGALKRFRHITLPLLSPTTLFLFVTTFIAS
                     MKVFQSIDVMTAGGPYESTNVMVYWIYDLAFVQFRVDRAAVVASVFFVILLAFTAATM
                     KISNKSVHYDS"
     gene            704..>1208
                     /locus_tag="PLQ29_14285"
     CDS             704..>1208
                     /locus_tag="PLQ29_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPG87861.1"
                     /translation="MTNQALAANVRARRLRAAATVLQYALAIAIVLIVVFPLYWMIIS
                     SLKTSEELLRPVPSLWPAEFRWRNYSDALAMAPFGRYFFNTVVMTVGIMALQLVIGSF
                     AAYGFAKGRFPGRDALFVVVLGALMIPIQVTFVPIYGMVAKLHWINTFAGLIVPHAVS
                     AYMIFLLR"
BASE COUNT          190 a          407 c          355 g          256 t
ORIGIN      
        1 ctggctcgcg tcgctcaagg tgacgttcac ctataccttc tgggagatcg tcataacgct
       61 ggtcggaggc atcctgctcg ccgcgctgtt caaccgcgag ggccgggtct tcgccgcgat
      121 gcgctcgatc gtgttcatgc ccaagtacat cgcgatatcg acctcggcca tcgtgttcat
      181 gtggatactg aacgaccagt acggcttcct gaactacgtg ctcggcgcct tcggagtcgg
      241 gccggtcgcg tggctcaact cggagcggac cgccctgacc tcggtgctga tcctgaccgg
      301 ctggcgcgtc gtcggctacg gcatgatgat ctacctgtcg gcgatgaagg gcatctccag
      361 ggactactac gaggccgccg agctcgacgg cgccggggcg ctcaagcgct tcaggcacat
      421 cacgttgccg ctcttgtcgc cgacgacgct gttcctgttc gtcaccacct tcatcgcgtc
      481 gatgaaggtc ttccagtcga tagacgtgat gaccgccggc gggccctacg agagcacgaa
      541 cgtgatggtc tactggatct acgacctggc cttcgtccag ttccgcgtcg accgggccgc
      601 cgtcgtcgcc agcgtgttct tcgtcatcct gctcgccttc acggccgcga cgatgaagat
      661 atcgaacaag agcgtccatt acgactccta ggagggaacg gcgatgacga accaagctct
      721 cgcggcgaac gtccgcgcca ggcggctcag ggccgccgcc accgtcctcc agtacgcgct
      781 cgcgatagcg atcgtgctga tcgtggtctt cccgctctac tggatgatca tctcgtcgct
      841 caagaccagc gaggagctgc tgcggcccgt gccgtcgctc tggccggccg agttccgctg
      901 gcggaactac tccgacgcgc tcgcgatggc gccttttggc aggtacttct tcaataccgt
      961 cgtgatgacg gtcggcatca tggccctgca gctggtcatc ggctcattcg cggcctacgg
     1021 cttcgccaag ggtcgcttcc ccggccgcga cgccctattc gtcgtggtgc tcggcgccct
     1081 catgattccg attcaggtca ccttcgtacc gatctacggc atggtcgcca agctccactg
     1141 gatcaacacc ttcgccggtc tcatagtgcc gcacgcggta tcggcctaca tgattttcct
     1201 cctcaggc
//
LOCUS       DAOPHV010000780         1201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_173825_length_1201_cov_2.416230, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000780 DAOPHV010000000
VERSION     DAOPHV010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173825_length_1201_cov_2.416230"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..741)
                     /locus_tag="PLQ29_14290"
     CDS             complement(154..741)
                     /locus_tag="PLQ29_14290"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPG87862.1"
                     /translation="MRKLPLVATILAALLLTGASAFAAEAPGANASRPAAVDARSVIV
                     VDMANGEVLYERDADLVIPPASLTKIMTMMVALEAVDSGRVGLSDRVLITRDDVTLPY
                     RSSLMYLQEGMSVPFDDLLRGMAVISGNDAAKTVARVLAGSQEAFAGLMNAEAERLGV
                     STGRLVRDLATDPALWQAVNAGRSRCGHRPLRADG"
     gene            complement(738..>1201)
                     /locus_tag="PLQ29_14295"
     CDS             complement(738..>1201)
                     /locus_tag="PLQ29_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPG87863.1"
                     /translation="ADAIAELERRKEPFEFKPYWGCDLQTEHERYLSETVVGGPVIVT
                     DYPKELKAFYMRLNDDGKTVRAMDVLVPRIGEIIGGSEREYRYDVLVENMRAKDLAPE
                     DYWWYLDLRRFGSAPHAGFGLGFERLLRYVTGIENIRDVIPFPRAVKQADF"
BASE COUNT          180 a          393 c          429 g          199 t
ORIGIN      
        1 ggggtctccc gccgccgctg ccctcgtggc agtggggcga cgatcgggga gacaggggcg
       61 aggcgtcgcc cctcttccca cgagtcttcg cgccaccgag acgagctcgg tggccgcgtc
      121 tcggcctcga cgtcgcccgt agactggagt cggtcagccg tcggcgcgca acgggcggtg
      181 gccgcagcgg ctgcgaccgg cgttgacggc ctgccagagg gctggatcgg tggccagatc
      241 ccggaccagg cgtccggtgc tgaccccgag ccgttcggcc tcggcgttca taaggccggc
      301 gaaggcctct tgcgagcccg cgagcaccct cgcgacggtc ttggccgcgt cgttgcccga
      361 tatgaccgcc atgccccgga ggaggtcgtc gaagggcacg ctcatgccct cctgcaggta
      421 catcagcgat gaccggtagg gcagggtcac gtcatccctg gtgatgagta cgcggtcgga
      481 gagcccgacg cggcccgagt cgacggcttc gagcgcgacc atcatggtca tgatcttcgt
      541 gaggctggct ggcgggatga cgaggtcggc gtcgcgctcg tatagtacct cgccgttagc
      601 catgtcgacc acgatgacgg acctggcgtc gacggcggcc ggacgagagg cgttggctcc
      661 cggcgcctcg gccgcgaagg cggaggcgcc ggtcaggagc aaggcggcta ggatcgtcgc
      721 gacgagagga agctttctca aaagtccgcc tgcttcacgg cccgcgggaa ggggatcacg
      781 tccctgatgt tctcgatgcc cgttacgtag cggagtaggc gctcgaagcc gaggccgaag
      841 cccgcatggg gcgccgagcc gaagcgcctg aggtccaggt accaccagta gtcctcaggc
      901 gccaggtcct tggcgcgcat gttctcgacg agcacgtcgt agcggtactc gcgctccgag
      961 ccgccgataa tctcgccgat gcgcggcacc agtacgtcca tcgcgcgcac ggtcttgccg
     1021 tcgtcgttca gccgcatgta gaaggctttg agctccttcg ggtagtcggt cacgatcacg
     1081 gggccgccga cgaccgtctc ggacaggtat cgctcgtgct cggtctggag gtcgcagccc
     1141 cagtagggct tgaactcgaa gggctccttg cggcgctcca gctcggctat ggcgtcggcg
     1201 t
//
LOCUS       DAOPHV010000781         1199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_174200_length_1199_cov_3.771853, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000781 DAOPHV010000000
VERSION     DAOPHV010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174200_length_1199_cov_3.771853"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..600
                     /locus_tag="PLQ29_14300"
     CDS             133..600
                     /locus_tag="PLQ29_14300"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPG87864.1"
                     /translation="MSHEITKSNADRIRLTIAPKGGGSENMSAMAMLKPSDGAAGVRR
                     FVVDTVDKAGPNPCPPVIVGVGVGGTIETAALIAKRSLLRAVGERHPDPRVARLEEET
                     LAEINALGIGPQGFGGTVTALDVHIEAFPAHIASLPVVVNLQCHAARHLEAVL"
     gene            597..1175
                     /locus_tag="PLQ29_14305"
     CDS             597..1175
                     /locus_tag="PLQ29_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HPG87865.1"
                     /translation="MSASGSHSGRLAIRLSLPIVGDDARSLRAGDRVLLSGVLYTGRD
                     VAHRRLVELLDRGGALPFDPSGQVIYYVGPCPAAPGRVIGSAGPTTSGRMDAYAPRLL
                     DAGLRGMIGKGPRSRAVIEAMTRNGAVYFAAIGGAGALLARAIKEAEVVAFGELGPEA
                     IYRLRVEDFPVTVAVDSTGTDLYAIGREPYRR"
BASE COUNT          205 a          399 c          415 g          180 t
ORIGIN      
        1 tcctgcattt cggcgcgcgc gatgcgcgta tccagcgcgc gctgcacctg gtgctcgaag
       61 cccgaatagg cgtggacgac ataccaacgc ttggccacgt actgaactcc ttattgaccc
      121 agcaagagcc ggatcagcca cgaaataacg aaatcgaacg ccgacaggat caggctgacg
      181 atcgcgccca agggcggcgg cagcgagaac atgagcgcga tggcgatgct caagccgtcg
      241 gacggcgcgg ccggcgtcag gcgcttcgtg gtagataccg tcgacaaggc cggaccgaat
      301 ccctgcccgc cggtgatcgt cggcgtcggc gtcggcggca ccatagagac ggcggccctg
      361 atcgccaagc gctcattgct gcgagccgtg ggcgaacgcc atcccgatcc cagggtcgcg
      421 aggctcgagg aggagacgct cgccgagatc aacgctctcg gcatcgggcc gcagggcttc
      481 ggcggcacgg tcaccgccct cgacgtccat atcgaggctt tcccggccca catcgcgagc
      541 ctgccggtcg tcgtcaacct gcagtgccac gcggcccgcc acctggaggc cgtcctatga
      601 gcgcgtccgg atcgcatagc ggacggctgg cgatccgtct gagccttccg attgtcggcg
      661 acgacgcgag gagcctcagg gccggcgacc gcgtcctgct gtccggcgtc ctctataccg
      721 gcagggacgt tgcccacagg cgcttggtcg agctcttgga taggggaggg gcgctcccgt
      781 tcgacccgtc cggccaagtc atctactacg tgggcccgtg cccggcggcc cccggcaggg
      841 tcataggctc ggcaggcccg acgacgagcg gccgcatgga cgcctacgcg ccgaggctgc
      901 tcgacgccgg gctcaggggc atgataggca agggcccgag gagccgggcc gtgatagagg
      961 cgatgacgcg gaacggcgcg gtctacttcg ccgcgatagg cggcgccggc gccctgctcg
     1021 ccagggcgat caaggaggcc gaggtcgtcg ccttcggcga gctcgggccg gaggcgatct
     1081 acaggctgcg cgtcgaggat ttcccggtca ctgtcgccgt cgattccacg ggaacggact
     1141 tatatgctat aggtagggag ccgtatcggc gatagaccta cgaggagtac ccatgtccc
//
LOCUS       DAOPHV010000782         1198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_174399_length_1198_cov_4.352581, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000782 DAOPHV010000000
VERSION     DAOPHV010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174399_length_1198_cov_4.352581"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PLQ29_14310"
     CDS             complement(<1..600)
                     /locus_tag="PLQ29_14310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669361.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPG87866.1"
                     /translation="MKKVYSHIESIKGSVITVRATGVTYGELAEVKTRFGVSLAEVNR
                     LDEDMVSLQVFAGGRGISTGDEVRFLGNPMRVSFGDSLKGRIFTGSGEPRDNGPTLSE
                     NLIPIGGPSVNPTNRIIPREMVRTGIPMIDLFNTLVKSQKLPIFSISGEPYNQLLARI
                     AMQAEVDLIILGGMGLKYDDYLFFKDTLEEGGSLQKTIMF"
     gene            complement(606..>1198)
                     /locus_tag="PLQ29_14315"
     CDS             complement(606..>1198)
                     /locus_tag="PLQ29_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPG87867.1"
                     /translation="TVSPAGGNFEEPVTQATLKVVGAFHGLSRERSDARKYPAIHPLD
                     SWSKYGGLLDASSVAWAHSFLKRGTEVGQMMKVVGEEGTSLDDFLVYLKGEFLDDVYL
                     QQNSFDAVDASVSPERQKASFRSCVRILGSSFDFKDKDAARAWFNQLRQRFLDGNGIE
                     WGTDAYVSAEAELKAFIDERVSGLDKKAARIIESLE"
BASE COUNT          212 a          376 c          389 g          221 t
ORIGIN      
        1 gaacatgatc gtcttctgga gggagccgcc ctcctcgagg gtatccttga agaacaggta
       61 gtcgtcgtac ttgaggccca taccgccgag gatgatcagg tcgacctcgg cctgcatcgc
      121 gatacgggcc agtagctggt tgtacggctc gccggagatc gagaagatcg gcagcttctg
      181 gctcttcacg agcgtgttga agaggtcgat catcgggatg ccggtgcgca ccatctcgcg
      241 cgggatgatg cggttcgtcg ggttgaccga cgggccgcct atcgggatca ggttctcgct
      301 cagcgtcgga ccgttgtcac gaggctcgcc cgagccggtg aagatacggc ccttgagcga
      361 gtcgccgaag ctgacgcgca tcgggttgcc caggaaacgc acctcgtcgc cggtcgagat
      421 gcctcggcct ccggcgaaga cctggagcga gaccatatcc tcgtcgagcc tattgacctc
      481 ggcgagcgaa acgccgaagc gcgtcttgac ctcggccagc tccccatagg tcacgccggt
      541 ggcccgcacg gtgatgacgc tgcccttgat cgattcaatg tgggaatata ccttcttcat
      601 atccgttact ccaggctctc aatgatccgg gccgccttct tatcgagccc cgatacccgc
      661 tcgtcgatga aggccttgag ctcggcctcg gccgacacgt acgcgtcggt accccactcg
      721 atgccgttgc cgtcgaggaa gcgctgcctg agctggttga accaggcccg ggcggcgtcc
      781 ttgtccttga agtcgaacga cgagccgagg atgcgaacgc aggagcggaa cgacgccttc
      841 tgccgctccg gcgacacgga tgcgtcgacc gcgtcgaacg agttctgctg caggtacacg
      901 tcgtcaagga actcgccctt gagatagacg aggaagtcgt cgaggctcgt gccttcctcg
      961 ccgacaacct tcatcatctg gccgacctcg gtgccgcgct taaggaagga atgcgcccag
     1021 gctaccgatg acgcgtcgag caggcctccg tacttggacc aggagtcgag cgggtggatc
     1081 gcggggtact tgcgggcgtc ggagcgctcg cgggacaagc cgtggaaggc gccgaccacc
     1141 ttgagcgtgg cctgggtgac cggctcctcg aagttgccgc cggccggcga caccgtgc
//
LOCUS       DAOPHV010000783         1198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_174409_length_1198_cov_4.050744, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000783 DAOPHV010000000
VERSION     DAOPHV010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174409_length_1198_cov_4.050744"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..656
                     /locus_tag="PLQ29_14320"
     CDS             153..656
                     /locus_tag="PLQ29_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87868.1"
                     /translation="MDVSEKTRDTWILAGAMALIVAASGFASSYASGQAARQLAKMTS
                     EALGPSPRGEATWTTMREGSVLGRVDRRGEPAAYLVVARYADGEFRAAASVDGDGSIL
                     ELKPLGDSGDAYSKRLAVLFSGAVRRGSRADASTLDASLKALVTDTFEALAILERGRT
                     EADDGDR"
     gene            640..>1198
                     /locus_tag="PLQ29_14325"
     CDS             640..>1198
                     /locus_tag="PLQ29_14325"
                     /inference="COORDINATES: protein motif:HMM:NF014557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HPG87869.1"
                     /translation="MMVIDNGRVSAGPLASLAGLAPLLVFSNSLSQGVALGVAFFLAY
                     SAAGAMALLLPARLGRSLVFMFSILGAAVVSSLTASALRLFDPFLFEAACDRVFLSVF
                     TLPVLRACVMPETVSDRERAIERLLWGIGYSFLIAVTGAAREFLATGAVSTRFGTERG
                     SLLPIFAQPAGAFMLIALAAAAFTVV"
BASE COUNT          177 a          383 c          404 g          234 t
ORIGIN      
        1 cgcctcgata ggtgcgctga gcttcatatt caggcgctgg ggcgccttcg ccgaaggcgt
       61 cgcgtacgcc gtgctcgtca tgaacgtgac ggtgccgttc atcgagaagg ccgccgcgag
      121 cctgcggctg aggcgcggga gggtcgcgta gtatggacgt atccgaaaag acgagggata
      181 cctggattct ggccggcgcc atggcgctaa tcgtggcggc ctcgggcttc gcctcctcgt
      241 acgcttccgg gcaggcagcc aggcagctgg ccaagatgac gagcgaggcg ctcggcccgt
      301 cgccccgggg agaagctacc tggacgacta tgcgcgaggg gagcgtactc ggcagggtag
      361 acaggcgggg cgagccagcg gcctacctgg tggtggctcg gtacgccgac ggtgaattca
      421 gggccgcggc cagcgtcgac ggcgacggat ccatactcga actgaagccc ctcggggatt
      481 ccggcgacgc ctattcgaag cggctcgccg tgctgttctc cggcgcagtc aggcgcggtt
      541 cgcgggcgga cgcctcgacg ctcgacgcga gtctcaaggc gctcgttacc gatacgttcg
      601 aggcgctggc aatactggaa cgaggcagga cggaggctga tgatggtgat agataacggg
      661 cgcgtatcgg ccgggccctt ggcctccctc gccggactag caccgctcct ggtgttctcg
      721 aacagcctgt cgcagggcgt cgcgctcggc gtcgccttct tcctagccta tagcgccgct
      781 ggcgccatgg cattgctcct gccagcgcgg ctcggccgct ccctcgtgtt catgttctcg
      841 atcctaggcg cggccgtcgt ctcgtcgttg acggcctcgg ctttgcgcct attcgacccg
      901 ttcctgttcg aggccgcctg cgatagggtt ttcctatcag tttttacgct cccggtactg
      961 cgcgcctgcg tcatgccaga gacggtttct gaccgggagc gcgcgataga gcgcctctta
     1021 tggggtatag gctattcatt cctgatagcc gtaaccggcg ctgccaggga attcctagcc
     1081 accggagccg tctcgacgcg tttcggcact gaacgcggct cgctgctacc catcttcgca
     1141 cagcccgccg gggcgttcat gctcatagcc ttggccgcgg ccgcattcac ggtggtta
//
LOCUS       DAOPHV010000784         1195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_175221_length_1195_cov_2.182456, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000784 DAOPHV010000000
VERSION     DAOPHV010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175221_length_1195_cov_2.182456"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLQ29_14330"
     CDS             <1..581
                     /locus_tag="PLQ29_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPG87870.1"
                     /translation="YFRSIRTDEEKLFQRVKASVEKGHFDEALSICDNNESPLSALIK
                     VGIEYRAYPESAQKEVLKDAAAQEVPRLERNVSTLGTIAHIAPLLGLLGTVTGTMKAF
                     GVLGRFGSVADPSILAKGISEALITTVGGIVVAVPAVIFYNLLVTKVNLILLKMENQV
                     TALVMMINSGKRRGLASAAAAVKEEAGRGAGA"
     gene            578..997
                     /locus_tag="PLQ29_14335"
     CDS             578..997
                     /locus_tag="PLQ29_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPG87871.1"
                     /translation="MTIERRLKPQINIDLTPLIDIIFQLVIFFMISSTFKTAPGIELQ
                     LPDSGTATAITITELSVVAVSEDEVYVNKTRTTAQGAESVIRLELEGKQVDEVQAVLE
                     AGAEAPYQLVVTLLDSLRRNGVDSVGLSTRTTAEEAP"
     gene            994..>1195
                     /locus_tag="PLQ29_14340"
     CDS             994..>1195
                     /locus_tag="PLQ29_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87872.1"
                     /translation="MTSARDEWLRARERKRATVAVSSALGAYLLLFGVAAILSLFAVQ
                     DMADFSGPIIVRLGSPDGVDAPH"
BASE COUNT          220 a          399 c          384 g          192 t
ORIGIN      
        1 tgtacttcag gagcatcagg accgacgaag agaagctgtt ccaacgggtc aaggcctcgg
       61 tggagaaggg tcatttcgac gaggcccttt ccatctgcga caacaatgag tcgccgctct
      121 cggcgctgat caaggtcggc atcgagtacc gtgcgtaccc cgagtccgcc cagaaggaag
      181 tactcaagga cgccgccgcc caggaggtgc cgagactcga gcgcaacgtc tcgacgctcg
      241 gtacgatagc gcacatagcc ccgctccttg gcctactcgg caccgtgacc ggcaccatga
      301 aggccttcgg cgtgctcggg cgcttcgggt cggtcgccga cccgtccatc ctggccaagg
      361 gcatctctga agcgctgatc accacggtcg gcggcatcgt ggtagccgtg ccagcggtga
      421 tcttctataa cctgctcgtc accaaggtca acctgatcct gctcaagatg gagaaccagg
      481 tgaccgccct cgtcatgatg atcaactccg gcaagcgccg cgggctggcg tcggccgcgg
      541 ccgccgttaa ggaagaggcg ggccggggag ccggcgcatg acgatagaac gcaggctcaa
      601 gccccagata aacatcgacc tgacgccgct gatcgacatc atcttccagc tggtcatctt
      661 cttcatgata tccagcacct tcaagaccgc gccgggcata gagctgcagc tgcccgactc
      721 cggtaccgcc accgctatca ccatcaccga gctgtcggtc gtcgccgtat ccgaggacga
      781 ggtctacgtc aacaagacgc ggaccaccgc ccagggcgcg gagtcggtga taaggctgga
      841 gctcgagggc aagcaggtcg acgaggtgca ggccgtgctc gaggcgggcg ccgaggcgcc
      901 gtaccagctc gtcgtcacgc tgctcgactc gctcaggagg aacggcgtag actcggtcgg
      961 cctgtcgacg cggaccaccg cggaggaagc cccatgacca gcgcccgcga cgagtggctg
     1021 cgcgcccgcg agcggaagcg cgcgaccgtg gcggtaagct cggcgctcgg ggcgtatctc
     1081 ctgctgttcg gcgtggccgc gatcctgtcg ctgttcgccg tgcaggacat ggccgacttc
     1141 tccgggccga tcatcgtacg gctcggctcg cccgacggcg tcgacgcgcc gcatc
//
LOCUS       DAOPHV010000785         1192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_175819_length_1192_cov_3.935796, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000785 DAOPHV010000000
VERSION     DAOPHV010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175819_length_1192_cov_3.935796"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            882..>1192
                     /locus_tag="PLQ29_14345"
     CDS             882..>1192
                     /locus_tag="PLQ29_14345"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPG87873.1"
                     /translation="MRSLFSVDMLTLFPYVASGGFSLGLVLIALLNGKRRDEVTWFVA
                     VLALEAAWAATFLLEVMASTTAAKLVWDALHWPLIVATDASILLFARAYVDPVGSRHG
                     SA"
BASE COUNT          208 a          349 c          375 g          260 t
ORIGIN      
        1 gatccttgtg acgagtacgt gatgcaccgt ggcaagtacg tgatgcaccg tgacgagtac
       61 gtgatactta gttattagga tatagcgctt aatgcctggt tcgacctact caaggacgat
      121 gcatagatgc agtaaggcaa aacccagccg cgctatgcca ggttcgtgat gctcaagcat
      181 gaatccgtgc tacatcatcg caaagacatg atgcggagtc gccggaatgt gatgctcaag
      241 catgaatccg tgctacatca tcgcaaagac gtgatgcgga gtcgccggaa cgtgagctgg
      301 agtcgccgga acgtgatgcg gagtcgccgg aacgtgagct ggaatgcctg ggcgctgact
      361 tggactcatg cggacgcggt cgtacttgat tgattcgtct ctccgacgcc gaatgggagc
      421 gttggcgcgc tcctctacgt cgagcggaac gtccggcggg gcgacgacgc agacgcctcg
      481 ccgcgtatct actcagccgt ttcttgcgag acaagggtgg atgcgaggcg cgacgccgga
      541 tgggagcgaa ggcgtactcc tgtacgtcga gggcagcgtc ccgcggcgcg acgacgcaga
      601 cgtctcgcct cgcaagaaaa gctattcttc gtgcttcgag gcggccttct cccgcttctc
      661 cttgcgcttc tccagtagcg tcttctgggc tttcttcttc ggttccttgg cttgcttgct
      721 cttctccgcc atatgaatcc tcctgggttg ggccgtagtc atgaacggcc gagttaccgg
      781 atatagtata ccccgctctc ggtacggcgg caacggaggg gcgtgaagac gggccgctcg
      841 aggcttgcgg atcgaccggc gatccgctac ctttagcgac gatgcgtagt ttgttctccg
      901 tcgacatgct taccctgttc ccttacgtcg cttccggcgg attctccctc gggctggtcc
      961 tcatcgccct gctcaacggc aagcgcaggg acgaggtaac ctggttcgtg gcggtgctcg
     1021 cgctcgaggc ggcctgggcg gcgaccttcc tgctcgaggt gatggcctcg acgacggccg
     1081 ccaaactggt ctgggacgcg ctacactggc cgctgatagt ggcgaccgat gcgtccatcc
     1141 tgctgttcgc tcgagcctac gtggatccgg tcggctccag gcacggctcg gc
//
LOCUS       DAOPHV010000786         1186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_177198_length_1186_cov_3.328028, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000786 DAOPHV010000000
VERSION     DAOPHV010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177198_length_1186_cov_3.328028"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..393)
                     /locus_tag="PLQ29_14350"
     CDS             complement(10..393)
                     /locus_tag="PLQ29_14350"
                     /inference="COORDINATES: protein motif:HMM:NF015889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015661 - L-lysine efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903401 - L-lysine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HPG87874.1"
                     /translation="MGAFAQILVLLAFMAAGALIHRVRLSPPAKVTDRLIKMVLWVLL
                     LVMGFRLGNARELRDRLGEIGLLAVGTALATLAGTIAAICAAYSLLDAMRTRRGGHRA
                     GAPGARPDGGSGPDRRVAAAGLGTF"
     gene            complement(396..1064)
                     /locus_tag="PLQ29_14355"
     CDS             complement(396..1064)
                     /locus_tag="PLQ29_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87875.1"
                     /translation="MDDSIRLRKDDDPRLSGSLVCMVLAEVDDMAPAPRALSGALGEL
                     AARRASKDFPPAAVKEPVRAMLRAGGFKPAGRQKPASEYLARAARDGVFPSINGPVDC
                     NNLLSLETGLPISLLDADELGGSALIRVCGAGEAYVFNASGQEMDLAGLLCVCAADGS
                     PLGNPVKDSMRGKLKDGTRRLAGFIYAPLGLYSPASLERVGERFAALLREHCGATARV
                     AGTV"
BASE COUNT          188 a          423 c          410 g          165 t
ORIGIN      
        1 gacggcccct taaaatgttc ccagcccagc cgcggcgact cgtcggtccg gccctgagcc
       61 gccgtcgggc cgcgcgccgg gcgcgccggc gcgatggccg cctcgacggg ttcgcatcgc
      121 gtcgaggagc gagtaggccg cgcagatcgc cgctatcgta ccggcgagcg tggcgagcgc
      181 cgtgccgacc gccaacaagc ctatctcgcc gagccggtcg cgcagctcgc gcgcgttccc
      241 aagcctgaag cccatcacga gcagcaggac ccagagtacc atcttgatca aacggtcggt
      301 caccttggcc ggcggcgata gcctgacgcg gtggatcagc gcgccggcgg ccatgaaggc
      361 caggaggacg agtatctgcg cgaaggcgcc catgcctaga ccgtcccggc gaccctggcc
      421 gtcgcgccgc aatgctcgcg gagcagggcc gcgaagcgct cccccacccg ttcgaggctc
      481 gctggcgagt ataagccgag aggcgcgtag ataaagccgg cgagccgcct cgtgccgtcc
      541 ttgagcttgc cgcgcatcga gtccttgacc gggttgccga gcggcgagcc gtcggcggcg
      601 cagacgcaca ggagcccggc caagtccatc tcctggccgg aggcgttgaa cacgtaggcc
      661 tcgccggccc cgcagacgcg gatcagggcc gagccgccga gttcgtcggc gtcgagcagg
      721 ctgatgggca gcccggtctc gagcgacagc aggttgttgc agtcgaccgg cccgttgatc
      781 gacgggaaga cgccgtcgcg ggcggcccgc gccaggtact cgctggccgg cttctgtcgg
      841 ccggccggct tgaagccccc cgcgcgcagc atcgcccgca ccggctcctt gacggcggcc
      901 ggcgggaagt ccttcgaggc tcggcgagcg gccagctcgc cgagcgcgcc cgaaagggcg
      961 cgcggcgccg gggccatgtc gtcgacctcg gccaggacca tgcacacgag cgatcccgac
     1021 agccgcgggt cgtcgtcctt acgtaatcgt atcgagtcgt ccataacgcg acggtatcac
     1081 gccgccgctc cgtccgcaag cgaccggaag gttctccttg atacccggcc ggtagatgct
     1141 atacttatgc catgcccagg aggattcgat gatcaagcga gcctgt
//
LOCUS       DAOPHV010000787         1180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_178663_length_1180_cov_2.755556, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000787 DAOPHV010000000
VERSION     DAOPHV010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178663_length_1180_cov_2.755556"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            436..789
                     /locus_tag="PLQ29_14360"
     CDS             436..789
                     /locus_tag="PLQ29_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87876.1"
                     /translation="MMIAVENKTIDSLSRPGSDRVNILYVTATDGVLTVTVSAYLTAH
                     TAGDLRIDVSINNSFGQYRARRILVGEQAHELLNMALDLAYSEHCDGEVGKMACSNAI
                     IEYVTKDKLEINAAR"
     gene            874..1179
                     /locus_tag="PLQ29_14365"
     CDS             874..1179
                     /locus_tag="PLQ29_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87877.1"
                     /translation="MMREIKVSDQDREAAKRIGHEMNSFSVSSQGVARAIYQNEHPTL
                     IQGIMRVSMQLIWDMAEEGRYHDDRTAASAKIAKAIKKALVEQGLGTEDFPPALPFI"
BASE COUNT          305 a          283 c          371 g          221 t
ORIGIN      
        1 tgtaggcctt gaacggcgcg taaagggctt ctagctggag gctatccgac agctagaacg
       61 agcaggcgcg ttcgacagga aaataccccc agacccgatg aacctgtggg cggcggacgg
      121 cggcatgatc cgcgaaccat cgcaagcagc gggagggctg gcctgagagg ggcaagtgag
      181 ttcgggcagt aggtgggcgg gcgaataacg cctgcatagc gcggggaacc ggcgaaggac
      241 gcctgaaaaa aggccatgta gcagcagttt tgatgtttta tggccgggta ctgaggaggg
      301 aagagggata ccttcttccc tcctccacct gggggagcgc gactagggcg aacatgccaa
      361 acctgcagta taacccatgt tttgggggct gacctagcag ggcaggtgtg ggcatgatgg
      421 catggaggtg cgaacatgat gatagcggtg gaaaacaaga ccatcgattc cctgagcagg
      481 ccgggaagtg accgcgttaa tatcttatac gtaacggcga ccgacggagt cctgacggta
      541 acggtatccg cgtacctaac ggcgcatacg gcgggcgatc tgcggataga cgtttcgatt
      601 aacaattcat tcggacagta cagggcgcgg cgtattctcg taggcgaaca ggcccatgaa
      661 ctgctgaata tggcattaga ccttgcttat tccgaacatt gcgatggcga agtagggaaa
      721 atggcttgtt caaatgccat catcgagtac gtaaccaagg ataagctgga gataaacgct
      781 gcccgctaag ttggtgcgga gaatgcccgc acaagggaag gccagcgcaa caggccttcc
      841 ctttcgttta tgggcaaccg aaaggagcga tatttgatgc gagagataaa ggtttccgac
      901 caggaccgcg aggcagccaa aaggatcggc cacgaaatga actcgttttc cgtttcttcc
      961 cagggcgtgg cgagggctat ttaccagaac gagcatccta cgctgataca gggcatcatg
     1021 agggtatcaa tgcagctcat atgggacatg gccgaagaag gcaggtatca cgacgaccgt
     1081 accgccgcat ccgcaaagat agcgaaagct ataaaaaagg ccctagtgga acagggtttg
     1141 gggacagaag actttcctcc tgcgctgccg ttcatatagc
//
LOCUS       DAOPHV010000788         1179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_178884_length_1179_cov_3.192171, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000788 DAOPHV010000000
VERSION     DAOPHV010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178884_length_1179_cov_3.192171"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PLQ29_14370"
     CDS             complement(<1..932)
                     /locus_tag="PLQ29_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HPG87878.1"
                     /translation="MSGNVFDRVLNAAGEALIIAELGTGHGGDELKAMELAAAAVEAG
                     ADCVKLQHVYADEIIHPETGLVPLPGGLTRLYDRFLAVETGPDFIGRVKDRVESLGAE
                     FLCTPFGLRSARELRELGVRAMKVASPELNHLPLLDELASYGLPTILSSGVSTLADIE
                     LALSRFHGVRTALLHCVTAYPAPEADYNLRLLPALSALFGLPVGVSDHSSDPILVPAL
                     AVALGAAIIEKHFCLSRSDDGLDDPIALPPAEFAAMASAIRARPSLDGLRAEYGSARV
                     EATLGDGVKRLAPSERANYTRTNRSIHAKRRIRAG"
BASE COUNT          228 a          374 c          381 g          196 t
ORIGIN      
        1 cccgctcgaa tgcgcctctt cgcgtggata gaccggttcg tacgcgtata gttggcccgc
       61 tcgctcggag ccaggcgctt gacgccatcg ccgagcgtcg cctcgactcg tgccgaaccg
      121 tactcggcgc ggagaccgtc gaggctcggc cgcgcgcgta tggccgacgc catcgcggcg
      181 aattcggctg gcggcagggc tataggatcg tccaggccgt catcgctacg cgacaggcag
      241 aaatgcttct cgatgatcgc cgcgccgagg gctacggcga gcgccggtac caggatcggg
      301 tcggacgaat gatcggacac gccgacggga aggccgaaca gcgcggacag cgcaggcaac
      361 agcctgaggt tgtagtcggc ctcgggggcg ggataggcgg taacgcaatg gagcagggcc
      421 gtccgaaccc cgtgaaagcg gctgagcgct agctcgatgt cggctagcgt cgataccccg
      481 gaggacagga tggtcggcaa tccgtatgag gccagttcgt cgagcaacgg caggtggttg
      541 agctccgggc tggcgacctt catcgcgcgc acgcctagct cgcgtaactc gcgggcgctt
      601 cgaaggccga acggcgtgca caggaactcg gcaccgagcg actcgacgcg atccttgacg
      661 cgcccgatga agtccggccc cgtctccacg gccaggaacc ggtcgtacag gcgggtcagc
      721 ccgccgggta acggcacgag gcccgtctcg ggatgtatga tctcgtcggc gtagacatgc
      781 tgcagcttga cgcaatccgc cccagcctcg accgcggcgg cggccagttc catcgccttg
      841 agctcgtcgc caccgtggcc ggtgcccagc tcggcgatga tcaaggcctc gccggccgcg
      901 ttcaagacgc gatcgaatac gtttccgctc atcttagctc cgttccagcg ccagaacgct
      961 attcctctcg acaatcgacg ccctatacta tagaatcgtc ggcatcgaca gcaatcgagg
     1021 agagtcaata tgaagcactt attctgcgat gtctgcaaga aagaagtagt cgatcccatc
     1081 ccgacgagga cgttcttcca catacgcgag ttcgatctat gcgaggcctg ccatgacgac
     1141 ctcgaggcgg cgatcaagta tactgtgcgc ggaaagaag
//
LOCUS       DAOPHV010000789         1175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_179938_length_1175_cov_2.422321, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000789 DAOPHV010000000
VERSION     DAOPHV010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179938_length_1175_cov_2.422321"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PLQ29_14375"
     CDS             <1..221
                     /locus_tag="PLQ29_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87879.1"
                     /translation="EPVSVFDIYEGDPDELRSRKDLTRAAFERGIEAFHEHRLGDART
                     LFSQVLATIPDDGAALRYLNAIGERDPA"
     gene            complement(262..1011)
                     /locus_tag="PLQ29_14380"
     CDS             complement(262..1011)
                     /locus_tag="PLQ29_14380"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPG87880.1"
                     /translation="MEALWDAIAKTVRVDQADPVAAWRAHQAALNARLAFLNGHAFKA
                     LRYRNAAGTDLTIALPAGHVWFGGGDDIVGAAETYAFVANMPTEEVFTMPRRDGANGR
                     IVSTMPLNYNGNLVEDFWFRLEDGVVVDFGARKGAEVLKEMLDTDEGARRLGEVALVP
                     YESPISRMGILFYNTLFDENASCHFALGKAYPTCIAGGPEMSKQELLAAGVNDSLIHV
                     DFMVGSKDLEIVGVMADGSEVPVFTNGNFAL"
BASE COUNT          184 a          397 c          395 g          199 t
ORIGIN      
        1 ccgagccggt gtcggtcttc gatatctacg agggcgaccc ggacgagctg cgttccagga
       61 aggacctgac ccgagcggcc ttcgagcgcg gcatcgaagc cttccatgag caccgcctcg
      121 gcgacgcccg cacgctattc agccaggtac tggcgaccat ccccgacgac ggggccgccc
      181 tgcgctacct gaacgccatc ggggagcgcg acccggccta gcgcgccggg tcgcgccgcg
      241 ctcgctatag cgccgcgctc gctatagcgc gaagttaccg ttcgtgaaga cggggacttc
      301 ggagccgtcg gccatcacgc cgactatctc gagatccttg gaaccgacca tgaagtcgac
      361 gtggatcagc gagtcgttga cgccggcggc gagcagctcc tgcttgctca tctcggggcc
      421 tccggcaatg caggtcgggt aggccttgcc gagggcaaag tggcacgagg cgttctcgtc
      481 gaacagcgtg ttatagaaca ggatgcccat cctcgagatc ggcgactcgt agggcacgag
      541 ggccacctcg ccgagcctgc gcgcgccttc gtcggtgtcg agcatctcct tgagcacctc
      601 ggcgcccttc ctcgcgccga agtcgacgac gacgccgtcc tcgaggcgga accagaagtc
      661 ctcgaccagg ttgccgttgt agttgagggg catggtgctg acgatcctgc cgttcgcgcc
      721 gtcgcgtcgc ggcatcgtga acacctcctc ggtgggcatg ttggccacga aggcgtaggt
      781 ctcggcggcg ccgacgatat cgtccccgcc gccgaaccag acgtggccgg caggcagggc
      841 tatcgtaagg tcggtgccgg ccgcgttacg gtagcgcagg gccttgaagg cgtggccgtt
      901 caggaaggcg agcctggcgt tcaaggcggc ctggtgggcg cgccacgcgg cgaccgggtc
      961 ggcctggtcg acgcggaccg tcttggctat agcgtcccag agggcctcca tggcctccta
     1021 caccccgctg gtcccagtgg cccactacga cttggtgggc ttcacaccgg aagctctggc
     1081 cacccggcct gaactgccgc ctgcgattcg cgtggcctgc ttcgtgctcc agcgcagccc
     1141 catcgcgtcg gcattgatgg acgagctgag cttga
//
LOCUS       DAOPHV010000790         1171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_180802_length_1171_cov_3.662186, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000790 DAOPHV010000000
VERSION     DAOPHV010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180802_length_1171_cov_3.662186"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /gene="tpx"
                     /locus_tag="PLQ29_14385"
     CDS             <1..306
                     /gene="tpx"
                     /locus_tag="PLQ29_14385"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HPG87881.1"
                     /translation="KESAGALVLTVSRDLPFAQRRFCEAEGIDAVITLSELRDRSFGK
                     DYGVELIDGPMAGLLSRAVVVADATGRVVYVEQVPEIKREPDYGKALDAVRAALAGY"
     gene            382..591
                     /locus_tag="PLQ29_14390"
     CDS             382..591
                     /locus_tag="PLQ29_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HPG87882.1"
                     /translation="MKKILTIEGMSCGHCAMRVKKALEEVAGVSSATVDISEGKAVVE
                     GEAPDPNMLRDAVEKAGYVVTGVLP"
     gene            complement(663..>1171)
                     /locus_tag="PLQ29_14395"
     CDS             complement(663..>1171)
                     /locus_tag="PLQ29_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HPG87883.1"
                     /translation="FTGVVEEVKPEKQKLKVTIPPGIDDGKRLSVSGQGDAGQNGGRP
                     GDLYVFIHVKPHEFFERDGYDLYCAVPVSVMKATLGGEITVPTIDGKRVKVSVPAGSQ
                     HGKMLRLREEGVPGAGGSAKRGDMYIKLMVEVPQKLSKRGRELLEELMSVEGDVTEPK
                     PIRLSDLK"
BASE COUNT          199 a          368 c          381 g          223 t
ORIGIN      
        1 aaggagtcgg ccggcgccct ggtcctgacc gtcagccgcg acttgccgtt cgcgcagcgg
       61 cgattctgcg aggccgaggg catcgatgcc gtcataacgc tgtcggagct acgcgaccgg
      121 tctttcggga aggactacgg cgtcgagcta atagacggac cgatggccgg gctcctatcc
      181 agggccgtcg tcgtcgcgga cgcgacgggc cgcgtggtct acgtcgagca ggttccggag
      241 atcaaacgtg aacccgatta tggcaaggca ctcgacgcgg tccgggccgc gttggcgggt
      301 tactgacctc tacctattct cattgctccg tagtcgtgat atattatacc ctataggggt
      361 attatagacg aaggagagcc tatgaagaag atactgacca tcgagggcat gagctgtggg
      421 cattgcgcca tgcgggtcaa gaaggccctc gaggaggtcg ccggcgtttc gtccgcgacg
      481 gtggacataa gcgagggcaa ggccgtggtc gagggcgagg cgcctgaccc gaacatgctg
      541 cgcgacgcgg tagagaaagc cggctacgtc gtcaccggcg tgctccccta ggctcgcggc
      601 accggaatac gtcgaagccc cggactccgc tggagcccgg ggcttcgacg taatgagtcg
      661 tattacttaa ggtcggatag cctgatcggt ttcggctcgg tcacgtcgcc ctcgacgctc
      721 atcagctcct cgaggagctc gcggccacgc ttcgacagct tctgaggcac ttcgaccatg
      781 agcttgatgt acatgtcgcc gcgcttggcg ctgcctccgg cgccaggcac gccttcctcg
      841 cgcagccgga gcatcttacc gtgctgcgat ccggccggca cgctcacctt gacccgcttg
      901 ccgtctatcg tcggcacggt gatctcgccg ccgagcgtgg ccttcatcac ggagacgggc
      961 acggcgcagt agagatcgta gccgtcgcgc tcgaagaact catgcggctt gacgtggatg
     1021 aatacgtaca ggtcgccggg ccggccaccg ttctggcccg cgtcgccctg ccccgagacc
     1081 gataggcgct tgccgtcgtc gatgcccggc gggatggtca ccttgagctt ctgcttctcg
     1141 ggcttgacct cttcgaccac gcccgtgaag t
//
LOCUS       DAOPHV010000791         1169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_181383_length_1169_cov_2.636445, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000791 DAOPHV010000000
VERSION     DAOPHV010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181383_length_1169_cov_2.636445"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="PLQ29_14400"
     CDS             complement(<1..1147)
                     /locus_tag="PLQ29_14400"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPG87884.1"
                     /translation="MGYLVLGVSPDVTLRGGVGAAAIAALVLISLAFLRVGRLKTAIY
                     SYLISTVVIVSAVRIYGAFTDPETAFTAYIYYMLYVIVFVAAFGERSHVAPAVVVFVL
                     NNVATLLIVRRAEGVSAATATGFVNGTIGMAITGVSAFSLVSLMDSYTSQLRANAEAA
                     SDKMKKIEEAVSTVRDGLDVGERLVSEAKSMEDSLGEIDRSLEASQGRLASLADEIAE
                     AKGANDEIVRASSDLGVSSDSYRAISVQASAAVNQMTASIQEISSITGRGGRSMESLS
                     ESIARGEGAADLSSESMERLSGNADSILSIVDVINGIASQTNLLAMNAAIEAAHAGDS
                     GKGFSVVAEEIRRLAEETADNISAITEGLKAFMDDLGQANQANRGIGK"
BASE COUNT          196 a          401 c          366 g          206 t
ORIGIN      
        1 ccttccctat tccgcggttg gcttggttag cctggccaag gtcgtccatg aaggccttga
       61 ggccctcggt gatcgcgctg atattatcgg cggtctcctc ggcgagacgc cggatctcct
      121 cggctaccac gctgaaaccc ttgccggagt cgccggcgtg ggccgcctcg atcgcggcgt
      181 tcatcgcgag caggttggtc tggctggcga ttccgttgat cacgtcgacg atggacagga
      241 tggagtcggc gttgcccgac agccgctcca tcgactcgga cgacaggtcg gcggcgccct
      301 cgcctctcgc gatgctctcc gacaggctct ccatgctccg gccgccccgc ccggtgatcg
      361 acgatatctc ctggatcgac gcggtcatct ggttcaccgc ggccgacgcc tgcaccgaga
      421 tggcccggta cgagtcgctg gagacgccca ggtccgagct ggccctgacg atctcgtcgt
      481 tagcgccctt cgcctcggct atctcgtccg ccagcgacgc gagccggccc tggctggcct
      541 cgaggcttcg gtcgatctcc ccgaggctat cctccatcga cttcgcctcc gatacgagcc
      601 gctcgccgac gtcgaggccg tcgcgaaccg tcgacacggc ctcctcgatc ttcttcatct
      661 tatcggacgc ggcctccgcg ttggccctca gctgcgatgt gtaggagtcc atcaggctga
      721 cgagcgagaa ggccgagacc ccggtgatcg ccatgcctat tgtcccgttg acgaagccgg
      781 tcgccgtcgc ggcgctgacg ccttcggccc gccgcacgat cagcagcgtc gccacgttgt
      841 tgaggacgaa gacgacgacg gccggcgcga cgtgactgcg ctcgccgaag gcggcgacga
      901 agacgatgac gtacagcatg tagtagatat aggcggtgaa cgcggtctct ggatcggtaa
      961 aggcgccgta gatgcgcacc gccgacacga tcactaccgt gctgatcaaa tacgaataga
     1021 tggccgtctt gagccgccct acgcgtagga acgccaggct gatcagcacg agcgcggcga
     1081 tagccgccgc gccgacgcct ccccgcagcg taacgtcggg agacacgccc agcaccaggt
     1141 atcccacggg aataaagcag agcaggaac
//
LOCUS       DAOPHV010000792         1165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_182199_length_1165_cov_4.629730, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000792 DAOPHV010000000
VERSION     DAOPHV010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182199_length_1165_cov_4.629730"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLQ29_14405"
     CDS             complement(<1..364)
                     /locus_tag="PLQ29_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG87885.1"
                     /translation="MPKPPPLLSLQHISKSFYGNPVLEDVSFDVGPGEIVGLVGENGA
                     GKTTLMRILFGMPVIAETGGYGGRIELDGAQVSFKTPFDALDAGIGMVHQEFSLIPGF
                     STTENIVLNREPMKANAAC"
     gene            complement(540..>1165)
                     /locus_tag="PLQ29_14410"
     CDS             complement(540..>1165)
                     /locus_tag="PLQ29_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HPG87886.1"
                     /translation="TFAFETAPDPTSDVGVAGAQQFILEKVPAWIEKYGKETAFFCTN
                     DAHTEPLLKQLLQYGGYFVEADLPSPLMGYPGALGVDLTAEAGNFPAILKKVEQAVVD
                     KGGAGRFGTWAYSYGFTVTAGLGEFAKNVIEGKTTKDSLDGLFEAFAVYTPGAQWNGG
                     YYIDANTGVRAKNHLLVYMDTYILGKGYLPTTKQTVPEKYFSIKFKK"
BASE COUNT          199 a          338 c          401 g          227 t
ORIGIN      
        1 cgcaggcggc gttggccttc ataggctctc ggttgagcac gatgttctcc gtcgtgctga
       61 agcccggtat cagggagaat tcctggtgaa ccatgccgat gccggcatcg agcgcgtcga
      121 acggcgtttt gaaggagacc tgagccccgt ccagctcgat ccgaccgccg tagccgccgg
      181 tctcagctat cacgggcatg ccgaacagga tgcgcatcag cgtggtcttg ccggcaccgt
      241 tctcgccgac gagaccgacg atctcgcccg gcccgacgtc gaacgagacg tcctccagga
      301 ccgggtttcc gtagaagctc ttcgatatat gctgcaagga aagcaagggt ggtggtttgg
      361 gcatgtgtcc tacctttttt cgtgcgaggt cggccgtcct gggacgtcga taccgctggc
      421 cctgactgga tcggcgggct agcgagcccg cgcgcgagag gcatcataat cgatgccggc
      481 ggtattgtca aaaaaaatga gccgccccgg agggcggctc gatacggcgg gaaagggaat
      541 tacttcttga atttgatcga gaagtacttc tcgggcaccg tctgcttggt ggtgggcagg
      601 tagcccttac cgaggatgta ggtgtccatg tagacgagga ggtggttctt ggcgcggacg
      661 ccggtgttgg cgtcgatgta gtagccgccg ttccactggg cgcccggcgt atagacggcg
      721 aaggcctcga acaggccgtc caggctgtcc ttggtggtct tgccctcgat cacgttcttg
      781 gcgaactcgc cgaggccggc ggtgacggtg aagccgtagc tataggccca ggtgccgaaa
      841 cggccagcgc cgcccttatc gaccacggcc tgctcgacct tcttgaggat ggcggggaag
      901 ttccctgcct cggcggtcag gtcgacgccg agcgctcccg ggtagcccat gagcggcgac
      961 ggcaggtcgg cctcgacgaa gtatccgccg tactggagca actgcttgag gagcggctcg
     1021 gtgtgggcat cgttcgtgca gaagaaggcg gtctccttgc cgtacttctc gatccaggcc
     1081 gggaccttct cgaggatgaa ctgctgggcg ccggccacgc cgacgtcgga ggtcgggtcg
     1141 ggggcggtct cgaacgcgaa ggtca
//
LOCUS       DAOPHV010000793         1165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_182230_length_1165_cov_3.416216, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000793 DAOPHV010000000
VERSION     DAOPHV010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182230_length_1165_cov_3.416216"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1165
                     /locus_tag="PLQ29_14415"
     CDS             <1..>1165
                     /locus_tag="PLQ29_14415"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPG87887.1"
                     /translation="SCDRDASARVDVSPDRLRATLTVLKGRGRGKPLSLTMVSEALAG
                     RRLKGVKIDKLKTDVVAFYRGKETELEDYVLAVGKEPTKGKDRTLIYSVAFLPVPQAK
                     EYLEAVEAAPLLSKYAQRLDEFPLSAAGRVALVKKGQEIARFSQPSPGQAGVDVYGDA
                     IPAPPGNDPLVKAFDYVRFSKESIEGEEDGLVLIGDGEAETASVAEAKPSSGPGDSEP
                     GSEAAAAPKEPPKPSAILVRVLPYRDAKVAVTIAEDAMSAAVDVEKGYGLGRELTLEL
                     AQEALAQAGVTEGADLKELAAALNEAREGRIVSSRIIARGAPPVPAGGYRLNWLIRIA
                     SGAALTMRTDGSVDYKNQDRSTVVVEGQPLLELLAIGVEGQDGRDVLGGIIPAP"
BASE COUNT          212 a          380 c          398 g          175 t
ORIGIN      
        1 gtcctgtgac cgcgacgcct cggcgagggt cgacgtttcg cccgacaggc tcagagccac
       61 gctgacggtg ctcaagggtc ggggccgagg caaaccgcta tcgctgacga tggtcagcga
      121 ggctctggcc gggcgtcgcc tcaagggcgt caagatcgat aagctcaaga ccgatgtggt
      181 cgcgttctat agaggcaagg agacggaact cgaggactac gtcctggccg tcggcaagga
      241 gccgaccaag ggcaaagaca ggacgttgat ctatagtgtc gcgttcctgc cggtgcccca
      301 ggccaaagag tatctcgagg ccgtcgaggc ggcgcctctc ctatccaagt acgcccaaag
      361 gctcgacgaa ttcccgctct cggccgccgg ccgcgtcgct ctggtcaaga agggacagga
      421 gatagccagg ttcagccagc cctctcccgg gcaggccggc gtcgacgtat acggagacgc
      481 tattccggct cctcccggta acgatccgct ggtcaaggcc ttcgactacg tccggttctc
      541 caaggagtcg atagagggtg aggaggacgg gctggtcctt atcggcgacg gagaggccga
      601 gactgcgtcc gtggccgaag cgaagccctc ctccggtccc ggagactccg agcccggatc
      661 ggaggcagcc gcagctccga aggagccccc gaagccttcg gccattctcg tcagggtact
      721 gccgtaccgc gacgccaagg tcgccgtgac catagccgag gacgcgatgt ccgcggccgt
      781 cgacgtcgag aagggctatg ggctcggccg ggagctgacc ctggagctgg cccaggaagc
      841 gctcgcgcag gccggcgtga ccgagggcgc cgacctgaag gaactggccg ccgcgctcaa
      901 cgaagcccgc gagggccgaa tcgtttcgtc gcggatcata gcgcggggag ccccgccggt
      961 gccggccgga ggctaccgcc tcaactggct aattaggata gccagcggcg ccgccttgac
     1021 catgcggacc gacggatccg tcgactataa gaaccaggat cgctctaccg tcgtcgtcga
     1081 ggggcagcct ctgctcgagc tcctggccat cggcgtcgag ggccaggacg gccgcgacgt
     1141 gctcggtggg atcatccccg cgccc
//
LOCUS       DAOPHV010000794         1164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_182444_length_1164_cov_3.806132, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000794 DAOPHV010000000
VERSION     DAOPHV010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182444_length_1164_cov_3.806132"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1164
                     /gene="pyk"
                     /locus_tag="PLQ29_14420"
     CDS             <1..>1164
                     /gene="pyk"
                     /locus_tag="PLQ29_14420"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPG87888.1"
                     /translation="CSMNTQKLTKIVATISDSQCEPEFLRSLYEAGMNVVRLNTAHQT
                     PEASAKVVANIRKVSDTIGILVDTKGPEVRVKGLPAPLELKAGETVTIPKVDEVRKGF
                     SVSYAGFTAEVPVGSRILIDDGAIELTVKNVANGMLLCEVMNDGLLKDRKSVNAPGVP
                     LRIPALSDKDREYLKFVADNDVDFIAHSFVRNKDDVLEVRRILDGYGSKARIIAKIEN
                     IQGVTNLPEILDFADGAMVARGDLGVEIPFERLPGIQKTIIDACVRKGKPVITATQML
                     HTMIDNPRPTRAEVSDVANAVFDGTDALMLSGETAFGKYPLEAVRTMARIAVQAESQK
                     PRREAFVSEATPNRVRDYMARSVALSALSLPIKAIIADTESGRIARLISSYRSH"
BASE COUNT          236 a          390 c          373 g          165 t
ORIGIN      
        1 tgtagcatga acacccagaa actcaccaag atcgtcgcga ccatatcgga ttcccagtgc
       61 gagccggagt tcctccgctc gctctacgag gccggcatga acgtggtcag gctcaatacc
      121 gcccaccaga cgccggaagc ctccgccaag gtggtggcca atatcaggaa ggtgtccgat
      181 acgataggca tcctggtgga caccaagggc ccggaggtcc gcgtaaaggg cctgccggcg
      241 cccctggagc ttaaggccgg ggagacggtg accatcccca aggtggacga ggtgcgcaag
      301 ggcttctccg tgtcctacgc gggcttcacc gcggaggtgc cggtcggctc gcgcatcctg
      361 atcgacgacg gggcgatcga gctgacggtc aagaacgtcg cgaacgggat gctgctctgc
      421 gaggtgatga acgacggcct cctcaaggac cgcaagagcg tcaacgcgcc gggcgtgccg
      481 ctgcggatac cggccctgtc ggacaaggac cgcgagtacc tcaaattcgt agccgacaac
      541 gacgtcgact tcatcgccca ctcgttcgtg cgcaacaagg acgacgtgct cgaggtcagg
      601 cgcatcctgg acggctacgg cagcaaggcc aggatcatcg ccaagatcga gaacatccag
      661 ggcgtgacca acctgcccga gatactcgac ttcgccgacg gcgcgatggt cgcgcgcggc
      721 gacctgggag tggagatacc cttcgagcgc ctgccgggca tccagaagac catcatcgac
      781 gcctgcgtgc gcaagggcaa gccggtgatc accgccaccc agatgctcca cacgatgata
      841 gacaacccgc ggcccacgcg cgccgaggtc tccgacgtgg ccaacgccgt gttcgacggt
      901 accgacgcgc tgatgctctc cggcgagacg gccttcggca agtacccgct ggaggcggtg
      961 cgcacgatgg ccaggatagc cgtccaggcc gagagccaga agcccaggcg cgaggccttc
     1021 gtcagcgagg ccacgccgaa ccgcgtccgc gactacatgg ccaggagcgt ggcgctgtcg
     1081 gccctgtcgc taccgatcaa ggcgatcata gccgacaccg agtccgggcg tatcgcccgc
     1141 ctgatctcca gctaccgcag ccac
//
LOCUS       DAOPHV010000795         1160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_183526_length_1160_cov_2.794570, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000795 DAOPHV010000000
VERSION     DAOPHV010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183526_length_1160_cov_2.794570"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PLQ29_14425"
     CDS             complement(<1..988)
                     /locus_tag="PLQ29_14425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPG87889.1"
                     /translation="MAKRVSAAVRRVFSSVTGAAARFFKAGRQKLTIMLVPHSEKRIF
                     NLQISLFGLAAVIALALGAAGFLAYSTVGYGDVVGKLAGKTESLKATQADLDAIRDST
                     ARLVKSARSFQAALDSTLAGIGIKSQSDAASRADGDLAAFFSPSEAGSGSLREAAEIE
                     RVSAYLERSVKPLGELGDTIAAQGGIMSEIPNIWPIKGGIGHISMYYGQNENPIYGSW
                     YIHKGIDLSTFRSGDPILATADGKVVTVAYDISLGNYIIIEHKHGFLTRYAHLSAFKV
                     QKGAVVKQGQVIGYIGNTGLSTGPHLHYEVHLGTETIDPLRFLNIRSGSATLT"
BASE COUNT          207 a          332 c          396 g          225 t
ORIGIN      
        1 gcgtcaacgt agccgagccg gagcggatat tgaggaagcg gagcgggtcg atggtctcgg
       61 tgccgaggtg cacctcgtaa tggaggtgag ggccggtcga gaggccggta ttgccgatat
      121 agcctatcac ctggccctgc ttgacgacgg cgcccttctg gaccttgaag gccgaaaggt
      181 gggcgtagcg ggtcaggaag ccgtgtttat gctcgatgat gatgtagttg ccaaggctga
      241 tgtcgtaggc gacggtgacg accttgccgt cggcggtggc caggatgggg tctcccgagc
      301 ggaaggtgga caggtcgata cccttatgga tgtaccagga gccgtagatc gggttctcgt
      361 tctgcccata gtacatcgag atgtggccga tgccgccttt gatcggccag atattcggta
      421 tctccgacat gatgccgccc tgggccgcta tcgtgtcgcc gagttcaccg agcggcttaa
      481 cgctccgctc gaggtaggcg ctgacccgct cgatctcggc ggcttcgcgc aggctgcccg
      541 agccggcctc cgaaggggag aagaacgccg cgaggtcgcc gtcagcgcgc gaggcggcgt
      601 cggactggct tttgatgccg atgccggcaa gagtcgagtc gagggcggcc tggaaggagc
      661 gagccgactt gaccagcctg gccgtgctat cgcggatggc gtcgaggtcg gcctgggtgg
      721 ccttgaggct ctcggtcttg ccggccagct tgccgaccac atcgccgtag ccgacggtcg
      781 aatacgccag gaagccagcg gcgccgagcg cgagggcgat cacggcagct agtccgaaga
      841 ggctgatctg aaggttgaag atgcgcttct cggagtgggg gaccagcatg atggtaagct
      901 tctgcctacc cgccttgaag aaccgtgccg cggcgccggt aacggacgag aaaacgcggc
      961 ggaccgcggc gcttacgcgc ttggccaccg atttctctac atccttatat cgttgcagcg
     1021 tcgccaacgc aggcctccgg tctctataaa ttcgttcaac gataccacgt cgcggccgtc
     1081 gctgtcaaac cgctggcttc gcgcacgcct cgttttgaaa tcgctccgac cgtacagtat
     1141 agtatggttt atgaccgatg
//
LOCUS       DAOPHV010000796         1153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_185261_length_1153_cov_2.865209, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000796 DAOPHV010000000
VERSION     DAOPHV010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185261_length_1153_cov_2.865209"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PLQ29_14430"
     CDS             complement(<1..914)
                     /locus_tag="PLQ29_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87890.1"
                     /translation="MPEIDNRACPDDDTVSLVDLVAVLLKYRRLIIVSTLAAAALAMA
                     AYLIYPPYSLAKAESWRVVEVNASMMLESSLESSIGETEGLNFVTQSLLDTSNIVEAL
                     RGAGYETVERVAIGAGADEDKAFYTIRRRFVENRSANGSPLKETNRAYSVKVDKGVVS
                     IMFKNGDAEKAKAFIAGMIDLVRRDLCEFSRPKALSTIASYERLLAIPNPSEAVETSI
                     AQGYRDYSAAKALIEGASSPLTVLRRPYVLVPVLTVEVIRADLLKKCVLLVFGVFFLA
                     VFGAFVLNYVDTVKRDPEAMGKIRSALKR"
BASE COUNT          217 a          373 c          333 g          230 t
ORIGIN      
        1 cgcttcaggg cggagcgtat ctttcccatc gcctccggat cgcgcttgac cgtatcgaca
       61 tagttgagca cgaaggctcc gaacaccgct aggaagaaca cgccgaagac cagcagtacg
      121 cacttcttga gcaggtcggc tcgtatcacc tcgacggtaa gcaccggcac gagcacgtac
      181 ggcctgcgca ggacggtcaa gggcgagctg gcgccttcga tcagggcctt ggctgccgaa
      241 taatcacgat agccttgcgc tatgctcgtc tccaccgcct cgctcgggtt gggtatagcc
      301 aggagtcgct cgtacgacgc tatcgtcgac agcgccttgg gcctggagaa ttcgcacagg
      361 tcccgcctga cgaggtcgat catcccggcg atgaaagcct tggccttttc agcgtcgccg
      421 ttcttgaaca tgatcgagac gacgccctta tcgaccttga ccgagtacgc gcgattggtc
      481 tccttgagcg gcgagccgtt agccgacctg ttctcgacga agcgcctgcg tatcgtatag
      541 aacgccttgt cctcgtcggc cccggcaccg atggccaccc tctcgaccgt ctcatagccg
      601 gccccgcgca gagcctcgac gatattcgac gtgtcgagca ggctctgcgt cacgaagtta
      661 agaccctcgg tctcgccgat actcgactcc aggctcgact cgagcatcat gctcgcgttc
      721 acctctacca cgcgccagct ctcggccttg gccaggctat agggcgggta gatcaggtac
      781 gccgccatgg ccagcgcagc ggcggccagc gtcgaaacga taatcaggcg tcggtacttg
      841 agcagtacgg ccaccaggtc gacgagcgat acggtatcgt catcgggaca ggcgcgattg
      901 tctatctcag gcattatatt actccctcgg gaacggtgtg ctttctatca gaacgagact
      961 tccatctcga gtccgaatac cagagccagt cggcggtcgg ctacatcctc gttaaaagtc
     1021 gttacgtagc gagatccgtc cgggccgtag gctaccggta cggccacggt cgccgttata
     1081 tacttccacg gcttccaggt aacgcccgcg gtcgccgatc cggatcatcg ccttggttcc
     1141 ggccggggcg gcc
//
LOCUS       DAOPHV010000797         1149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_186220_length_1149_cov_3.332724, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000797 DAOPHV010000000
VERSION     DAOPHV010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186220_length_1149_cov_3.332724"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLQ29_14435"
     CDS             <1..321
                     /locus_tag="PLQ29_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87891.1"
                     /translation="ETDPVVQEAIAFGIMKELADDAKRGRIVGGIVNVATPLAAAGGH
                     AIAAAATGDWSDFDDRVIGTMSWTLPSLVSGIIMLVSGRSTEERMLDSYRAMSASYAA
                     GARD"
     gene            331..852
                     /locus_tag="PLQ29_14440"
     CDS             331..852
                     /locus_tag="PLQ29_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87892.1"
                     /translation="MSAWLKALTLGIAAALGLSRAAEPPPLTLRLSRDATTIYASVEV
                     YELPGTDLERLVEASFVVRLEATIWAGRAAVEAYRDIRYTGRYYEIRVSETGGVHRTA
                     DPAAAWAIASRFSRLPLGPATAVSFPLSAGCKATISLPEDPGHDPMVVWGYRAAADYR
                     EIDSLGHVPYWQP"
     gene            849..>1149
                     /locus_tag="PLQ29_14445"
     CDS             849..>1149
                     /locus_tag="PLQ29_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87893.1"
                     /translation="MRLRSGIFIVSFSVSVALLGGAAVAMTSFYRSKGDRSLASLEAA
                     TAELSAMARALAEGEAPDPDTMMEVLAPIRRDLTTRMVLERDAAVSSALAWAAFLG"
BASE COUNT          164 a          401 c          396 g          188 t
ORIGIN      
        1 gagaccgacc cggtcgtaca ggaggcgata gcgttcggca tcatgaagga gctggccgac
       61 gacgcgaagc gcggcaggat agtcggcggt atcgtgaacg tcgccacccc cctcgccgcc
      121 gccggcgggc acgcgatcgc agcggccgcc accggcgact ggagcgattt cgacgacagg
      181 gtcatcggta cgatgagttg gaccctgccg agcctcgtct cgggcatcat catgctcgtc
      241 tccggacgct cgaccgagga gcgcatgctc gactcgtacc gcgcgatgag cgccagctac
      301 gccgccgggg cgcgtgacta gcgtggcggc atgagcgcct ggctcaaggc tctcaccctc
      361 gggatagcgg cggccctggg cctctccagg gccgccgagc cgccgccgct gactttgagg
      421 ctgtcccgcg acgctacgac gatctacgcg tcggtcgagg tctacgagct gccggggact
      481 gatcttgaac gactcgtcga ggcgtcgttc gtcgtacggc tcgaggcgac gatatgggcc
      541 ggccgtgccg ccgtggaggc ctaccgcgac atccgctata ccggtcgcta ctatgagatc
      601 cgcgtctcgg agaccggggg agtccaccgt acggccgatc ccgcggcggc ctgggcgata
      661 gcctcgcgct tctcgcgcct gccgctcggc ccggcgaccg cagtatcgtt cccgctgagc
      721 gccggctgca aggcgacgat ctccctgccc gaggatcccg gccacgaccc catggtcgtc
      781 tggggctaca gagcggccgc cgactatcgg gagatcgact cgctcggcca cgtgccgtac
      841 tggcagccat gaggctgcgc tccggtatct tcatcgtgtc gttctccgtc tcggtagcct
      901 tgctcggagg ggccgccgtg gccatgacgt ccttctatcg gtcgaagggc gaccggtccc
      961 tggcttcgct cgaggccgcc acggctgaac tgtcggccat ggcccgcgcc ttggccgagg
     1021 gcgaggcgcc cgatcccgac acgatgatgg aagtcctagc gccgatcagg cgtgacctga
     1081 cgacgcggat ggtgctcgag cgcgacgcgg ccgtatcgtc ggccctggcc tgggccgcct
     1141 tcctcggcg
//
LOCUS       DAOPHV010000798         1148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_186472_length_1148_cov_5.743824, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000798 DAOPHV010000000
VERSION     DAOPHV010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186472_length_1148_cov_5.743824"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PLQ29_14450"
     CDS             complement(<1..267)
                     /locus_tag="PLQ29_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87894.1"
                     /translation="MIGPAVLAAALAGALLASCGRAPEAAAGPGPAVSAAAPPLSGVA
                     SPNDPGAAAPPPSARRMTVLACGDILLARTPGKRAAEYGFRYLFE"
     gene            complement(264..>1148)
                     /gene="nadE"
                     /locus_tag="PLQ29_14455"
     CDS             complement(264..>1148)
                     /gene="nadE"
                     /locus_tag="PLQ29_14455"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPG87895.1"
                     /translation="ADEATIAAFPDRWEEAERVLVEGIRGYMRKCGFKKAHLGLSGGI
                     DSAIVAVLAAKAIGPENLTCIAMPSRFSSTGSIDDSVELAANLRCRLETIPIEGPFSS
                     FLATMEPHFAGLPFGLTEENLQARVRGDILMAWSNKFDSMLLTTGNKSELAVGYCTLY
                     GDMCGALAPIGDLLKTEVFELCESVYAREGLLPRAIIDKPPSAELRPNQLDQDSLPPY
                     ELLDAVLRLYIEDDLAVDAIVALGFDEGLVRRVVSMTARAEYKRRQAAPVVKVSPRAF
                     GMGRRMPIARIVSEARPR"
BASE COUNT          190 a          393 c          397 g          168 t
ORIGIN      
        1 ctcgaacagg tagcggaaac cgtactcggc cgctcgctta cccggcgtcc tggcgagcag
       61 gatgtcgccg caggccagga cggtcatcct gcgggcgctc ggcggcggag cggcggcgcc
      121 gggatcgttc gggctcgcga cgcccgatag cggcggagcg gcggccgata cggcgggacc
      181 cgggccggcc gccgcctcgg gcgctcggcc gcacgaggcg agcaacgctc cggccaacgc
      241 cgcggccagg accgccgggc ctatcaccga ggccttgcct cggatacgat gcgcgcgatg
      301 ggcatacgcc gccccatacc gaaggcccgc ggagacacct tgacgaccgg ggccgcctgt
      361 ctgcgcttat actcggcgcg cgccgtcatc gatacgaccc ttcggacgag cccttcgtcg
      421 aagccgagcg cgacgatggc gtccacggcc aggtcatcct cgatatagag cctgagcacc
      481 gcatcgagca gctcgtaagg cggtagcgag tcctggtcga gctggttggg ccggagctcg
      541 gcggacgggg gcttgtcgat gatggcgcgg ggcaggagcc cctcgcgcgc atagacgctc
      601 tcgcacagct cgaaaacctc ggtcttgagc agatccccta tcggagccag ggcgccgcac
      661 atatcgccgt acagcgtgca atagccgacg gccagctcgc tcttgttacc ggtcgtcagg
      721 agcatcgaat cgaatttgtt ggaccaagcc atcaggatgt cgccccggac gcgcgcctgc
      781 aggttctcct cggtcaggcc gaacggcagg cccgcgaagt gcggctccat cgtcgccagg
      841 aaactcgaga acggcccctc tataggtatc gtctccaggc ggcagcgcag gttggccgcg
      901 agctcgacgc tgtcgtctat ggagccggtc gacgagaagc gcgacggcat ggcgatgcag
      961 gtcagattct ccggcccgat agccttggcg gccagcaccg cgacaatggc cgagtcaatg
     1021 ccgccggaca ggccgaggtg ggccttcttg aagccgcact tgcgcatgta gccgcggata
     1081 ccctcgacga ggacgcgctc ggcctcctcc caccggtccg ggaaggcggc gatcgtggcc
     1141 tcgtcggc
//
LOCUS       DAOPHV010000799         1147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_186775_length_1147_cov_3.312271, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000799 DAOPHV010000000
VERSION     DAOPHV010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186775_length_1147_cov_3.312271"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1147
                     /gene="tig"
                     /locus_tag="PLQ29_14460"
     CDS             <1..>1147
                     /gene="tig"
                     /locus_tag="PLQ29_14460"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPG87896.1"
                     /translation="GPQLKLDAMGRVMDEAVEKALDGEALVPLSYAQPELDGQPAFEL
                     DADFSFSVTYDVFPEVVAGDISGMELELPQVTIAKADEERELDEIRDRNAIVMDKDDK
                     AAAKMGDIATVDYAELDESGEPIKGTERQDFVFEIGSGHNLYKFDDDVIGMKKDAEKT
                     IEKTFPADFEYAELAGQTKKLRVKLTKLKEKKLPELDDELAQDVSEQFKTLDDLKADI
                     RAKLEKRLEDRLKSLREKGVVDALVEKSTVDLPASMVAAELEMRLRNLMQRMGIDSEE
                     RLAQIVSMSGRSVDQLREEWRPEAEKAIKTRLVLDKLTEEGKYAASDDDVAAEYAKMA
                     ADSNLSVDEVKAEYERRGMVEYLVDRIKEDRLIADLEKKMKVKKGKKI"
BASE COUNT          274 a          347 c          366 g          160 t
ORIGIN      
        1 gggccgcagc tcaagctcga cgcgatgggc cgcgtgatgg acgaagcggt cgagaaggca
       61 ctcgacggcg aggcccttgt cccgctctcc tacgcccagc ccgagctcga cggccagccc
      121 gccttcgagc tcgacgccga tttctccttc tccgtgacct acgacgtgtt ccccgaggtc
      181 gttgccggcg atatatccgg catggagctc gagctacccc aggtgaccat agccaaagcc
      241 gacgaggagc gagagctcga cgaaatccgc gaccgcaacg ccatcgtcat ggacaaggac
      301 gacaaggcgg cggccaagat gggcgacatc gccacggtcg actacgccga gctcgacgag
      361 tcgggcgagc cgatcaaggg taccgagcga caggacttcg tcttcgagat cggttccggc
      421 cacaacctgt ataagttcga cgatgatgtt atcggcatga agaaagacgc cgagaagacc
      481 atcgagaaga cctttcccgc cgacttcgag tacgccgagc tggccggtca gaccaagaag
      541 ctacgcgtca agcttaccaa gctcaaggag aagaaactcc ccgagctcga cgacgagctg
      601 gcccaggacg tatcggagca atttaaaacc ctcgacgacc taaaggccga catacgggcc
      661 aagctcgaga agcgactcga ggacaggctc aagagcctcc gtgagaaggg cgtcgtcgac
      721 gcgctcgtcg agaagtcgac cgtcgatctg ccggcctcga tggtcgcagc cgagctcgag
      781 atgcggctac gcaacctgat gcagcgtatg ggtatcgaca gcgaggagcg cctggcccag
      841 atcgtcagca tgagcggccg aagcgtcgac cagctacgtg aggagtggcg accggaggcc
      901 gagaaggcga tcaagacgcg cctcgtgctc gacaagctga ccgaggaggg caagtacgcc
      961 gcctcggacg acgacgtcgc cgccgagtac gctaagatgg ccgccgactc gaacctatcg
     1021 gtcgacgagg tcaaggcgga gtacgagcgc cgcggcatgg tcgagtacct tgtcgacagg
     1081 atcaaggaag accgcctgat agccgacctc gagaagaaga tgaaggtcaa gaagggcaag
     1141 aagatag
//
LOCUS       DAOPHV010000800         1146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_187016_length_1146_cov_4.703025, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000800 DAOPHV010000000
VERSION     DAOPHV010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187016_length_1146_cov_4.703025"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLQ29_14465"
     CDS             <1..287
                     /locus_tag="PLQ29_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPG87897.1"
                     /translation="FRSEKDTSIEEIDALLKKASETYLKGILGYGEEELVSSDFIHDQ
                     RSSIYDSLATKQNNLPGEKRFFKIVSWYDNEWGYSNRVVDMLRHMAGLKK"
     gene            354..>1146
                     /gene="pgk"
                     /locus_tag="PLQ29_14470"
     CDS             354..>1146
                     /gene="pgk"
                     /locus_tag="PLQ29_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPG87898.1"
                     /translation="MIRTIKDTSLSGKRVVMRVDFNVPMKDGVVQDDTRILAAIPSIE
                     YILAQGARSVTLMSHLGDPKKDSKKAKEKAEQAGKAWDEAAYIAGKHRMRPVADYLAA
                     KLGRPVVFAGEDGCYGKKAFVESQPDGTLIMLENTRFHKEETSSDPAERDAMAKELAG
                     YGEVFVNDAFGTAHRDHASTASIAKFVPVAVGGFLMDKEVRYLEPMVTNPPKPMVAII
                     GGAKVSSKIAVLESLIKNASVLIIGGGMAYTFLKAQGRSVGASLVE"
BASE COUNT          245 a          349 c          362 g          190 t
ORIGIN      
        1 ccttccgcag cgagaaggat acctcgatcg aagagatcga cgccctgctc aagaaggcca
       61 gcgagaccta cctcaagggc atccttggct atggcgagga ggagctcgtc tcctcggact
      121 tcatccacga ccagcgctcg tcgatctacg acagcctggc cacgaagcag aacaacctgc
      181 ccggcgagaa gcgcttcttc aagatcgtct cctggtacga caacgaatgg ggctattcga
      241 accgcgtggt ggacatgctc cgccacatgg ccggcctcaa gaagtagctg tagcctacct
      301 acggcccgcc tggcaggcgg gccttttttt cacgccgact acggaggaag atgatgatca
      361 ggaccatcaa ggatacgagc ctgtccggca agcgcgtcgt gatgcgcgtt gacttcaacg
      421 tgccgatgaa ggacggcgtc gtgcaggacg acacgcgcat cctagcggcg ataccgtcga
      481 tagagtatat cctggcccag ggggccagga gcgttacgct gatgagccac ctcggggatc
      541 ccaagaagga ttccaaaaag gctaaggaga aggcagagca ggcgggcaag gcctgggacg
      601 aggcggccta tatagcgggt aagcaccgca tgaggccggt ggcagactat ctcgccgcca
      661 agctcggacg cccggtcgta ttcgccggcg aggacggatg ctacggcaag aaggctttcg
      721 tcgagtccca gcccgacggc acgctgatca tgctcgagaa tacgcgcttc cataaggaag
      781 agacgagctc ggatccggcg gagcgcgacg ccatggccaa ggagctggct ggctacggcg
      841 aggtgttcgt caatgacgcc ttcggcacgg cccaccgcga ccacgcgtcg accgcctcca
      901 tcgccaagtt cgtgccggtc gccgtcggcg gcttcctgat ggacaaggag gtccgatacc
      961 tcgagccgat ggttacgaac ccgcccaagc cgatggtcgc catcatcggt ggcgccaagg
     1021 ttagctccaa gatcgcggtg ctcgagagcc tgatcaagaa cgcctctgtc ctgataatcg
     1081 gtggcggcat ggcgtatacc ttcctgaagg cccaagggcg ctcggtcggc gcttcgctcg
     1141 tcgaga
//
LOCUS       DAOPHV010000801         1146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_187023_length_1146_cov_4.159487, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000801 DAOPHV010000000
VERSION     DAOPHV010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187023_length_1146_cov_4.159487"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1146)
                     /locus_tag="PLQ29_14475"
     CDS             complement(<1..>1146)
                     /locus_tag="PLQ29_14475"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPG87899.1"
                     /translation="LRSKLGRARGIQGRLTVLFTGLILLIIVVLATSLLRDFGSTLLS
                     AIMDNGRSLADRGASIVKTNISDKIAVDDYLAIEAAKNEGAAFPFSSVTYYRRDPKSG
                     SYAARASTDPAIVGQAPSSVPEAGAAEAMIEAMIEAPGTIEFRAPVTLSGVNLGFVSV
                     VYDRQTIYGPYYLTTVKTAIISFLFLYASIFVTYLFGRGIVFPILFLRMSVNAIATRL
                     ASMVKGSERVSADSLRYDDRVATKDEIKKLSLEIGNMAAVIRGVVPYISASTLRHSER
                     EKPTTERRELAFLFTDIRGFTTICEGRSPEEVVAMLNHYLDIQTEAIVANGGDIDKFV
                     GDEVMAVFDGPEKELQACRAGMAIRKAMAEAQEQARKESGAVVSIGI"
BASE COUNT          192 a          367 c          388 g          199 t
ORIGIN      
        1 ctatgccgat ggacacgacg gcccccgatt ccttgcgcgc ctgctcctgg gcctcggcca
       61 tcgccttgcg gatggccatg ccggcgcggc aggcctgcag ctccttctcc gggccgtcga
      121 acacggccat cacctcgtca cccacgaact tgtctatgtc gccgccgttg gcgacgatgg
      181 cctcggtctg gatgtcgagg tagtggttga gcatcgcgac gacctcctcc ggggagcgcc
      241 cctcgcagat ggtcgtaaag ccgcggatat cggtgaatag gaaggccagc tcgcgccgct
      301 cggtcgtcgg cttctcccgc tcggaatggc ggagcgtcga cgcggagatg tacggcacga
      361 cgccgcgtat gaccgcggcc atgttgccga tctcgagcga cagcttcttg atctcgtcct
      421 tggtggccac gcggtcgtcg tagcgcaggc tatcggccga gacacgctcg gagcccttga
      481 ccatcgacgc cagccgcgtc gctatggcgt tgacgctcat gcgcaggaac agtatcggga
      541 acacgatgcc tcgcccgaac aggtaggtga cgaagatcga cgcgtagagg aacaggaacg
      601 agatgatggc ggtcttgacg gtcgtcaggt agtacgggcc gtagatcgtc tgacggtcgt
      661 agaccaccga cacgaagccc aggttgacgc cggagagcgt gaccggcgcc ctgaactcta
      721 tagtccccgg cgcctcgatc atcgcctcga tcatcgcctc ggcggcaccg gcctcgggca
      781 cggaggaggg cgcctgcccg acgatggcgg gatcggtcga ggcgcgggcc gcgtaggaac
      841 cgctcttagg gtcgcggcgg taatacgtta cgctggagaa cgggaacgcg gccccctcgt
      901 tcttggccgc ctcgatggcc aggtagtcgt ccacggctat cttgtcgctg atattcgtct
      961 tgacgatgct cgccccccga tcggcgagcg agcggccgtt gtccatgatg gcgctgagta
     1021 gggtcgagcc gaagtcgcgt aggagcgagg tcgcgagcac gacgatgatc aggagtatca
     1081 ggccggtgaa caggaccgtc agccgcccct gtatgccccg ggcccgcccg agcttggagc
     1141 gcaggc
//
LOCUS       DAOPHV010000802         1141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_188369_length_1141_cov_3.552486, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000802 DAOPHV010000000
VERSION     DAOPHV010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188369_length_1141_cov_3.552486"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="PLQ29_14480"
     CDS             complement(<1..1103)
                     /locus_tag="PLQ29_14480"
                     /inference="COORDINATES: protein motif:HMM:NF017106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HPG87900.1"
                     /translation="MIRGKRLALFAAMALALSAVPLPADDALDVDETEVSSAQGRAIQ
                     FINYEGPHEVIETAESIRGIGRALGRAVAAGATRSGEVGRYSVIHAVDPAVPAGFDAD
                     IILLGEGARVDHVKNVRRIVAGFLEEAYGYAPADADTLAVFVTIYNAVYRGDMGYFGA
                     RYKPVVTRELSPSTAGMSTRWDEWAGRTRIVIPLSGRAGMGVVGSVDTTPITDAPTVA
                     SLKDESPTAGVAERMDVVDIKERAQDEEKAAIEAEKLRLERETAAIAAEKAKAAEEKP
                     ATVAAAEAASQTGAAETGTETGTGTGREADGADGAAEKGGDQEASLAVSEKAREEEAA
                     AAERETSAREAQLEADKAALAAREEASAAKDAE"
BASE COUNT          128 a          435 c          383 g          195 t
ORIGIN      
        1 atctcggcgt ccttggctgc ggaggcctcc tcgcgtgcgg ccagggccgc cttgtcggcc
       61 tcgagctggg cctcgcgggc gctcgtctct cgctcggccg cggccgcctc ctcctcgcga
      121 gccttctccg acaccgcgag gctcgcctcc tggtcgccgc ccttctccgc ggccccatcg
      181 gccccgtcgg cctcgcgccc ggtcccggtc ccggtctcgg tcccggtctc ggcggcgcca
      241 gtctggctgg ccgcctcggc cgcggcgacc gtcgctggct tctcctcggc ggccttggcc
      301 ttctccgcgg ctatcgcggc cgtctcgcgc tcgagtcgca gcttctcggc ctcgatggcg
      361 gccttctcct cgtcctgggc ccgctccttg atgtccacca cgtccatccg ctccgccacg
      421 ccggcggtag gcgactcgtc cttgaggctc gctacggtcg gcgcgtcggt aatcggggtg
      481 gtatcgaccg agccgacgac acccatgccg gcgcggcccg acagcggtat gactatcctc
      541 gtacggccgg cccattcgtc ccagcgggtc gacatgccgg cggtggacgg ggacagctcc
      601 ctcgtgacga cgggtttgta acgggcgccg aagtagccca tgtcgccgcg atagacggcg
      661 ttatagatgg tgacgaacac ggcgagcgtg tcggcgtcgg ccggcgcata gccgtaggct
      721 tcctcgagga acccggccac gatgcgccgc acgttcttga cgtggtcgac gcgggcgccc
      781 tcgccaagga ggatgatatc ggcgtcgaag ccggcaggca cggcggggtc gaccgcgtgg
      841 atcaccgagt aacggccgac ctcgccggac ctggtagcgc cggcagcgac ggcccgcccg
      901 agcgcgcggc caataccgcg tatcgactcg gccgtctcga tcacctcgtg gggtccctcg
      961 tagttgatga actgtatcgc ccgtccctgg gcgctcgaga cctcggtctc gtcgacgtcc
     1021 agcgcgtcgt cggccgggag cgggacggcc gacagcgcca gggccatcgc cgcgaacaga
     1081 gccagtcgct tgcctcgtat catcccgtct ctcctcccgg cgcgagggcc gccgcgcttc
     1141 c
//
LOCUS       DAOPHV010000803         1141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_188503_length_1141_cov_2.100368, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000803 DAOPHV010000000
VERSION     DAOPHV010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188503_length_1141_cov_2.100368"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PLQ29_14485"
     CDS             <1..715
                     /locus_tag="PLQ29_14485"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPG87901.1"
                     /translation="TVSCCGDVLVAEDGGTMQVVAVLPDGRVKPLVQVVGHDGSEICG
                     PAFDPRTRLRLYSPDPRLREILEGQMKAPYFPVGLDDMAAGKEFIRLESGVDIGGVRI
                     MHRAMSHPGGCYSYAVEEAGKKAIYSTDTELDPKDFNRTPENESYFRGADALIIDAQY
                     TLGEAIEKQYWGHSSFSLAADFAASWNIKRLVLFHHEPAYGDQKIGSILKSASWYLKH
                     IEDHGIEVVLAREGVEIDV"
     gene            705..932
                     /locus_tag="PLQ29_14490"
     CDS             705..932
                     /locus_tag="PLQ29_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87902.1"
                     /translation="MTFNALYESTFEVELAGAELAGLYVSLTAAEERLDRNQLQALDR
                     LRSVLYENLSVEELESLPGLYTAAAGPGGSA"
     gene            929..>1141
                     /locus_tag="PLQ29_14495"
     CDS             929..>1141
                     /locus_tag="PLQ29_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87903.1"
                     /translation="MSRRTKVQGTGAVGKAAKAAFVVVALLVIAAVALGLGVIALAER
                     YDRPTGEVADEGLSFTVGQGESGSAVA"
BASE COUNT          213 a          359 c          390 g          179 t
ORIGIN      
        1 cacggtgtcg tgctgcggcg acgtgctcgt ggccgaagac ggcggcacca tgcaggtcgt
       61 ggcggtgctg cccgacggcc gcgtcaagcc cctggtgcag gtggtggggc acgacgggtc
      121 agagatctgc gggccggcct tcgacccgag gacgaggcta cggctgtaca gtcccgaccc
      181 caggctacgc gagatactcg aggggcagat gaaggcgccg tacttccccg tcgggcttga
      241 cgacatggcc gccggcaagg aattcatccg gctcgagagc ggcgtcgaca taggcggcgt
      301 acgcatcatg caccgcgcga tgagccatcc cggcggttgc tatagctacg ccgtcgagga
      361 agccggcaag aaggccatct actcgaccga taccgagctc gaccccaagg acttcaatcg
      421 gacgccggaa aacgagtcgt acttcagggg cgcggacgcc ctgatcatcg acgcccagta
      481 cacgctcggc gaggcgatag agaagcaata ctggggccat tcgtcgttca gcctggcggc
      541 cgacttcgcg gcctcatgga acatcaagcg gcttgtcctg ttccatcacg agccggccta
      601 cggcgaccag aagataggct cgatcctcaa gtcagcgtca tggtacctta agcatatcga
      661 agaccacggc atagaggtgg tcctggcgag ggaaggcgta gagattgacg tttaacgccc
      721 tgtacgagtc tacattcgaa gtcgagctgg ccggagctga gctggcgggc ctctacgtct
      781 cgctgacagc cgccgaggaa cggctcgaca ggaaccagct ccaggccctc gaccgcctca
      841 ggtccgtcct atacgagaat ctgtcggtag aggagctcga gagccttccc gggctctata
      901 ccgcggccgc cggcccgggg ggatcggcat gagccgacgt accaaggtcc agggaactgg
      961 cgcggtcggc aaggccgcca aggccgcctt cgtcgtcgtc gcgctcctcg tcatcgccgc
     1021 cgtggccctc gggctcggcg tcatcgcgct ggcggaacgc tacgacaggc cgacaggcga
     1081 ggtcgccgac gaggggttga gcttcacggt ggggcagggg gagtcgggct cggccgtagc
     1141 c
//
LOCUS       DAOPHV010000804         1126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_192385_length_1126_cov_3.055089, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000804 DAOPHV010000000
VERSION     DAOPHV010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192385_length_1126_cov_3.055089"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PLQ29_14500"
     CDS             <1..517
                     /locus_tag="PLQ29_14500"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPG87904.1"
                     /translation="GLADMMATIPTAERRDYAVVVDTPIWIKRYKLYAAPGHAALEAM
                     SRMRSVEDLKRSGLSVISYLGNDWSRATLERSGVRTINAGSVEGMYRMLLAGRADVAI
                     EDPILVERTLGALGLSGRLVETDGIVEQSAFHLMLSKRSPFAGLVDELDGALAEMRVD
                     GTIESIMSRYR"
     gene            complement(498..920)
                     /locus_tag="PLQ29_14505"
     CDS             complement(498..920)
                     /locus_tag="PLQ29_14505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPG87905.1"
                     /translation="MKKGAILCVDDEPIILLSLVQELKRTFGTDLAYERAMNAEAALA
                     TIESLEAEGVRVILIISDWLMPGIKGDEFLRIVHDRYPDIRAIMISGNADPEEMKEFR
                     KLESLVAVFGKPWDRKRLAEAIRSVLSAWKIEPSDNGS"
     gene            complement(945..>1126)
                     /locus_tag="PLQ29_14510"
     CDS             complement(945..>1126)
                     /locus_tag="PLQ29_14510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG87906.1"
                     /translation="ELTDEIWKPFFTTKGNGEGLGLGLDICRRLAERSGGRIYFESEP
                     GHTEFHIELPLVAGT"
BASE COUNT          192 a          353 c          355 g          226 t
ORIGIN      
        1 aggcctggcc gacatgatgg ccaccatccc cacggccgag cgccgcgatt acgcagtagt
       61 cgtcgatacg ccgatctgga tcaagcgcta caagctttac gccgcgcccg ggcatgcggc
      121 gctcgaggcg atgagccgca tgagatccgt cgaggacctg aagcgctcgg ggttgtcggt
      181 gatctcctac ctgggcaacg actggtccag ggcgacgctg gagcgctccg gcgtgaggac
      241 catcaacgcc ggctcggtcg agggtatgta ccggatgctc ttggctggcc gggcggacgt
      301 cgctatcgag gaccccatcc tggtggagag gacgcttggc gctctcggcc tgtcgggtag
      361 gctggtcgag accgatggca tagtcgaaca gagcgcgttc cacctgatgc taagcaagcg
      421 ctcccccttc gctgggctcg tcgacgaact ggacggcgcc ttggccgaga tgcgggtcga
      481 cggtacgata gagtccatca tgagccgtta tcgctaggct cgatcttcca ggcgctcagc
      541 acggatcgta tcgcctccgc caggcgtttc ctgtcccagg gcttgccgaa caccgcgacg
      601 agcgactcca gcttccggaa ttctttcatt tcctccggat cggcgtttcc gctgatcatg
      661 atggcccgaa tgtcggggta acgatcatgg actatcctga ggaactcgtc gcccttgatg
      721 ccgggcatga gccagtcgga gatgataagg atgacgcgca cgccttccgc ctccaaggac
      781 tcgatcgtcg cgagggccgc ctcggcgttc atcgcccgct cgtaggccag gtcggtcccg
      841 aaggtgcgct tgagttcctg taccagcgat agcaggatga tgggttcgtc gtcgacgcat
      901 aggatggcgc cttttttcac tgtaccctcc attttagaat gcgctcaggt gccggcgacg
      961 agcggcagtt cgatatggaa ctccgtatgc cccggctcgg actcgaagta tatccgccct
     1021 ccggagcgtt ccgccagcct gcggcagata tcgaggccca ggccgagccc ctcgccgttg
     1081 cccttcgtcg tgaagaacgg tttccatatc tcgtcggtca gctcgg
//
LOCUS       DAOPHV010000805         1124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_192913_length_1124_cov_3.095416, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000805 DAOPHV010000000
VERSION     DAOPHV010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192913_length_1124_cov_3.095416"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..>1124
                     /locus_tag="PLQ29_14515"
     CDS             347..>1124
                     /locus_tag="PLQ29_14515"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HPG87907.1"
                     /translation="MRRRTILVLILVTAFACLYGESRSTSVDFVLLVDTSLSMADAID
                     DAKRYAAGEVIGRLVEPGDWVSVLEFYGRSETVWQGDVRGEEDVAAIVRSLNSLVADG
                     RFTDIGGVLDEMDTLILARGQPDRPKYILLITDERQEAPEGTRYYAPDYVVRHPLLEY
                     VKRVDLGAFRVITIGYGLSSRIEGEARSLMTTLSEPPARREPTLPGAPSGVAAADGTA
                     SIGTEPPETVGPGAETGTSSGTSFGTGAATEATSRAADGSG"
BASE COUNT          222 a          331 c          337 g          234 t
ORIGIN      
        1 gaaccttatt cgtctcctcg tgcaggagct gatcgacctc tttctgctgg tctcggttct
       61 cctgtgccag agagtttcgg gagccgacgg cgctcggctt ctcgggatga taaacgttag
      121 gactcttggg aagatctatg gcgcccatgt ggttctcctt gcggtataat ttcgatactc
      181 gtcaatacta ccccgaaacg caaaaatgta aagctcttcg gctttttttc cactggatat
      241 gatttgaatt ttaatggaag atatcgttca tacgcctttt tagcttgcaa ataggtaaaa
      301 aataggcaat gctgtttctg tcaataaaat aacgatagaa ggcagcatga gaagacgaac
      361 catactagta cttatactcg taacggcgtt cgcctgcctg tacggcgagt cgcgatcgac
      421 gagcgtcgat ttcgtactgc tcgtcgacac atccctgtct atggccgacg ctatcgacga
      481 cgccaagcgc tacgcagccg gggaggtcat cgggcgtctc gtcgagccgg gcgactgggt
      541 aagcgtcctg gagttctacg gccgatccga gactgtctgg cagggagacg tacggggcga
      601 ggaagacgtc gccgccatcg tgcgtagcct gaactctctg gtcgccgacg gccgctttac
      661 cgacatcggc ggcgtcctcg acgagatgga taccctgatc ctggcccgag gtcagccgga
      721 tcgccctaag tacatcctgc ttatcaccga cgagcgccag gaggcgccgg agggcacccg
      781 ctattacgct cccgactacg tagtcaggca tccgctactc gagtacgtca agcgcgtcga
      841 cctgggggcg ttccgcgtca tcaccatagg ctacggcctc tcgtcgcgca tcgaaggcga
      901 ggcgcgctcg ctgatgacca ccctgtccga accgccggcc aggcgggagc cgactttacc
      961 cggtgcgcct tcgggcgtcg cggcggccga cggaacggcg agtatcggga ctgagccgcc
     1021 ggagacagtg ggtcccggag cggagaccgg aacctcgtcc ggaacctcgt tcggaacggg
     1081 cgccgctacg gaggcgacgt cgcgcgctgc cgacggttcc ggca
//
LOCUS       DAOPHV010000806         1124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_192920_length_1124_cov_2.961646, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000806 DAOPHV010000000
VERSION     DAOPHV010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192920_length_1124_cov_2.961646"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..673
                     /locus_tag="PLQ29_14520"
     CDS             116..673
                     /locus_tag="PLQ29_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87908.1"
                     /translation="MPVKAMDLFDAYQKSMLPSDQGFIVSSFFSPSSAYSRYEVVSYN
                     NVKSIYPADNGLTFQTDGKKLHILIEPANYPKKAEEPYVRSSQEQIPHRFSELDLHTC
                     KNQTKIYSSKEAIMSYTSFTVMKPAGVNFAFIFFNLPDVYESMTLFFEKTFNKEAGVP
                     MVDAKKVAKGLGVKIQDALKWEYTS"
     gene            complement(759..>1124)
                     /locus_tag="PLQ29_14525"
     CDS             complement(759..>1124)
                     /locus_tag="PLQ29_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPG87909.1"
                     /translation="GEIAKYSEFFSFGTNDLTQTTLGISRDDFNTFMPDYSQFDLVEG
                     NPFSNLVTPVKELVAMATRRGRMTRPSLHVGLCGEHGANPDNVRFCMEAGLDYVSCSP
                     YSVPIACLAVAQAELERSK"
BASE COUNT          259 a          320 c          319 g          226 t
ORIGIN      
        1 caacgagagg gccgcggcaa tcgagacggg cggcaagcct agaaagaaac gatagtcatt
       61 gagcggatcg ccattatatc gtatcctact atcagtactt ctcaggagga atcatatgcc
      121 ggttaaagcg atggacctgt tcgacgccta tcagaagagt atgctgccga gcgatcaggg
      181 cttcattgta tcgtcgttct tcagcccaag ctcagcctat tcacgttacg aggtggtctc
      241 gtacaataac gtcaagtcca tctatcccgc cgacaacggg ctgaccttcc agaccgatgg
      301 caagaagttg cacatcctga tcgagccggc caattatccg aagaaggccg aggagcccta
      361 cgtccgctcc agccaggagc agattccgca ccgcttttcc gagctagacc tgcatacctg
      421 taagaaccag acgaagatat actcgagcaa ggaagcgatt atgtcctata cctcgttcac
      481 ggtaatgaag ccggccgggg tcaatttcgc tttcatcttc ttcaacctgc ccgacgtcta
      541 cgaaagcatg acgctgttct tcgagaagac cttcaacaaa gaggccggcg tacctatggt
      601 cgatgccaag aaggtcgcca agggactcgg cgtcaagatc caggacgccc tcaagtggga
      661 gtatacgagc taggccggcc atagccgcgg caaattatcg ctatgataca agaagccgtc
      721 cgggaatccg gacggcttct tgcttactga gcggtggctt acttggagcg ctccagttcg
      781 gcttgggcca cggccaggca cgcgatcggc accgagtacg gtgaacacga gacatagtcc
      841 aggccggctt ccatgcagaa gcgcacgttg tcgggattgg cgccgtgctc cccgcagagc
      901 cccacgtgca ggctcggccg cgtcatacgg ccgcgcctcg tggccatggc gacgagttcc
      961 ttgaccggag tgactagatt ggaaaacggg ttgccctcca ccaggtcgaa ctgcgagtag
     1021 tcgggcatga aggtattgaa gtcgtcgcgg ctgatgccga gcgtcgtctg agtcaggtcg
     1081 ttcgtgccga agctgaagaa ctcgctgtac ttggcgatct cccc
//
LOCUS       DAOPHV010000807         1121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_193858_length_1121_cov_2.201689, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000807 DAOPHV010000000
VERSION     DAOPHV010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193858_length_1121_cov_2.201689"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..734)
                     /locus_tag="PLQ29_14530"
     CDS             complement(231..734)
                     /locus_tag="PLQ29_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87910.1"
                     /translation="MQVQIAIDTYDDLFSDFDIRDYRERFFSNDFLDELKMRTNRPLD
                     AKALAIKLVIPSDERDDGAEAIILDRIREFFAMRLERNKAKKRAILFTAVKFEAIGLL
                     IMLVANYLARFITDYLKDFLLIPSWFFVWSGLEKYMYNIRVLDKKIRFYDVLTRSSVT
                     FEGIEAR"
BASE COUNT          208 a          370 c          324 g          219 t
ORIGIN      
        1 tatcgacgcc gcgctccacc gccgcgtcta ccgcgcccac caggtcctcg ggcctcgcgt
       61 cgtcgagcag cggcttggcc ttccgggccg cctccgcgtc gcccccggcc agcatcgcca
      121 tgagcagctc gtcgaacccc gccgccctgt cggccgcgtt ccggattgcc tcagccatat
      181 cgcctccagt cgctccgtct ccgatagcga acgcgccttg cgcgacgcgc ctagcgcgcc
      241 tcgattccct cgaacgtaac cgaggatcgc gtcaggacgt cgtagaatcg gatcttctta
      301 tccaggaccc gtatattgta catgtatttt tccaagccgc tccagacgaa gaaccatgac
      361 ggaattaaaa ggaagtcctt caggtagtcc gtgatgaagc gggccaggta attggcgact
      421 agcatgatga gcaggccgat cgcctcgaat ttgaccgcgg taaacaggat cgcccgcttt
      481 ttcgccttat tccgctcgag ccgcatcgcg aagaattccc tgatcctgtc cagtatgatg
      541 gcttcggcgc catcatccct ttcgtccgag gggatgacga gcttgatggc cagggccttg
      601 gcgtcgaggg gcctgttcgt cctcatcttc agctcatcga ggaagtcgtt cgagaagaac
      661 cgttcccgat agtccctgat atcgaaatcc gagaacaggt cgtcgtaggt gtcgatcgcg
      721 atctgtacct gcatggccgc ctcccgaacg gacgcgccga gatacccgac cgtccatcca
      781 gcactatact atgatatcga acgctgaata gcgaggatcg acgataacgc ctccccgcgc
      841 ggcgcgccgt gtttctatcc ggggcggact gccgctatcc ttcctggggc ttcgcgcctc
      901 gtgctatagt ctcgccgcca gatcgaccga aagcgaggaa cgagggatgc ccatagccaa
      961 agcggtgcga gccagcctgg agcgcggctc gtggatacgg aagatgttcg aggccggaat
     1021 cgagctcaag gccaggctcg gcccggacgc cgtctacgac ttctcgctgg gaaatccgga
     1081 tctcgagccg ccggccgagt tcctggccgc gctcaaggcg g
//
LOCUS       DAOPHV010000808         1120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_194001_length_1120_cov_3.542723, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000808 DAOPHV010000000
VERSION     DAOPHV010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194001_length_1120_cov_3.542723"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..313)
                     /gene="rplT"
                     /locus_tag="PLQ29_14535"
                     /pseudo
     CDS             complement(1..313)
                     /gene="rplT"
                     /locus_tag="PLQ29_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261031.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
     gene            complement(449..649)
                     /gene="rpmI"
                     /locus_tag="PLQ29_14540"
     CDS             complement(449..649)
                     /gene="rpmI"
                     /locus_tag="PLQ29_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPG87911.1"
                     /translation="MPKMKTKRAASKRYTLSADGKVKYKKMNLRHILTKKSSKRKRKL
                     RKAGFVASGPVYQIKKKLLPYG"
     gene            complement(663..>1120)
                     /gene="infC"
                     /locus_tag="PLQ29_14545"
     CDS             complement(663..>1120)
                     /gene="infC"
                     /locus_tag="PLQ29_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPG87912.1"
                     /translation="ELGLDLVEIAPQSNPPVCKILDYGKFKFEQEKKLKDNRKHQKIV
                     KIKEVRMQPKIDDHDLDFKSKHVRTFLLEGNKVKVTIRFRGRELAHTEIGEEVLKSVL
                     TKIEGIYAMESPPRMEGRFMSMMLIPKAPAVKKPEPQRPQPDAAPETKA"
BASE COUNT          167 a          319 c          343 g          291 t
ORIGIN      
        1 gcggcgggat cgcgcacggc tatgtcggcc aggaccttgc ggtcgatcgc catgttcgcc
       61 ttggcggcag cctcgatgaa gcggctgtag gtgatgcccc ggccctgcac gcggcattga
      121 tgcgcgtgat ccatagggcg cggaaggtcg gcttcttgtc gcggcgatcg cgataggcgt
      181 acgacagggc cttggcgacc gcgtccttcg cggtcttatg gttggtggac ctgcgtccgt
      241 agtatccttt ggcaagcttg aggatcttct tgcggtgatc ctttcgcctg gtgccgtcaa
      301 ctgctctggg cattgctatt ctccttggaa aatatgaacc ggccgtcggg gaaaggtcct
      361 cgtcgaccgg cgcgccgtag cggcgccgag cctcatccta tcgtcgagtc cgccggcggc
      421 cctaacgggg gaaaggccgc ttgtcggatt aaccgtaggg gagaagcttc ttcttgatct
      481 gatatacggg gccggaggcc acgaagccgg ccttgcgtag cttgcgcttg cgcttcgaac
      541 tcttcttggt gaggatatgg cgaaggttca tcttcttgta cttgaccttg ccgtccgcgc
      601 tcagcgtata gcgtttagag gcagccctct tcgtcttcat cttaggcatt acgatccatc
      661 cttcaggctt tggtctctgg tgcggcgtcc ggctggggcc gctgaggctc gggcttcttg
      721 accgccggag ccttcggtat aagcatcatc gacatgaagc gcccttccat tctaggagga
      781 ctctccatgg cgtatattcc ttcgatcttg gtcagcacgc tcttcaggac ttcctcgcct
      841 atctcggtat gggcgagctc gcggcccctg aagcgtatgg tcaccttgac cttgttgccc
      901 tcgagcagga acgtgcgcac gtgcttggac ttgaagtcca ggtcgtggtc gtcgatcttg
      961 ggctgcattc ggacttcttt gatcttgacg atcttctggt gcttacggtt atccttgagc
     1021 ttcttttcct gctcgaactt gaatttgccg tagtcgagta tcttgcagac cggcgggttc
     1081 gattgcggcg ctatctcgac gaggtcgaga ccgagctcct
//
LOCUS       DAOPHV010000809         1119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_194368_length_1119_cov_2.636278, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000809 DAOPHV010000000
VERSION     DAOPHV010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194368_length_1119_cov_2.636278"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PLQ29_14550"
     CDS             complement(<1..885)
                     /locus_tag="PLQ29_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87913.1"
                     /translation="MSYALRWIMVAFLLAMAGIQIADPAQRTAGLSALYTLGVAVAFN
                     IAVALIIRRETRQAWLSWTAVTVDVLLVSSTIFSTAEHMHPSGAMTSAIVMLYPVVIT
                     IAAFRQRKRLVLYATGLSIAAYTGIYLTMVSRIDPALYELAPHLRPTGHFYKCMYLLG
                     FGAVLTVLPQTSRRLLATQQAAFEEATGKYERLAAELRSKLEALESGGKDLAEGMDRS
                     SSDLGRLSELASSAENGMASQGHAVVQVGSLMAELGPFSEALGRLIDDQGSSMREAAA
                     ATEEMLGNIQSIGNRVEQT"
BASE COUNT          204 a          375 c          334 g          206 t
ORIGIN      
        1 cgtctgctcc acccgattgc caatggactg gatgttgccg agcatctcct cggtagcggc
       61 ggccgcctcg cgcatcgacg agccctggtc gtcgatcagg cggccgagcg cctcggagaa
      121 cgggccgagc tcggccatca gcgagcctac ctgcacgacg gcgtggccct gcgacgccat
      181 gccgttctcg gcgctcgagg ctagctccga cagcctgccg aggtcgctcg agctcctgtc
      241 cataccttcg gccagatcct tcccgccgga ctcgagcgcc tcgagcttcg agcgcagctc
      301 ggctgccagg cgctcgtact tgccggtcgc ctcctcgaag gccgcctgct gcgttgcgag
      361 gaggcgcctc gacgtctgtg gcaacaccgt cagtaccgcc ccgaagccga gcaggtacat
      421 gcacttatag aagtgacccg tcggccgaag gtgcggcgcc agctcgtaga gggccgggtc
      481 gatgcggctg accatggtaa ggtagatgcc agtatacgct gctatcgaca gccccgtagc
      541 gtagagcact aggcgcttcc gctgcctgaa cgccgcgatc gtgatgacga ccgggtacag
      601 catcacgatc gccgaggtca tagcgccgga tgggtgcata tgctccgcgg tagagaatat
      661 cgtcgagctg acgaggagca cgtcgacggt caccgccgtc catgataacc atgcctgccg
      721 ggtctcgcgc cggataatga gggctaccgc tatgttgaac gccacggcca ccccgagcgt
      781 atacagcgcg gatagaccgg ccgtccgttg agccggatcg gctatctgaa tgccggccat
      841 cgccagcaaa aaggccacca tgatccagcg gagcgcgtac gacatccgct cgccgcgctc
      901 agactctcga ctgaaaatat catcctggga acccatcgat tgcctccggc cataccttgc
      961 atgaagccgg catcctcgtc aacgaaggat acgaccaatc gccttcggcg gccgggccgt
     1021 attgctgggt tcgccgtccc gcttgacttt tttctacgtt tcaggtaatt tctcctcgct
     1081 tccaacgaag cattcccctg tagctcagtt ggcagagca
//
LOCUS       DAOPHV010000810         1116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_195118_length_1116_cov_4.962300, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000810 DAOPHV010000000
VERSION     DAOPHV010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195118_length_1116_cov_4.962300"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /gene="lepB"
                     /locus_tag="PLQ29_14555"
     CDS             <1..606
                     /gene="lepB"
                     /locus_tag="PLQ29_14555"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPG87914.1"
                     /translation="LTEVEPDRWRGSPRGRLSAARIIVAAVALAVALKAFALDLVIVS
                     GESMSPTIGSGAVAVVAPCAYGLRLPLSGSYLVRWGEPAPGDIVLVSPFPPERRKAIK
                     RVFDVGPAFIVAEAGSLRGRGGVVSLGPSPRSSLAGTAYLPAGKAFVVGDNAERSIDS
                     RRYGAVPIEKLAGKVLLWFGGAAEPTLRTILSKDAAEDVDR"
     gene            593..>1116
                     /locus_tag="PLQ29_14560"
     CDS             593..>1116
                     /locus_tag="PLQ29_14560"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87915.1"
                     /translation="MSIDKRFIRHYVIIAALLAVAGSTLVRYARLAGGAGADRPSASA
                     SRVVSIRGPIYDREGRLLAVDTDLYDVSIWKPSFQADKVGSFARSASAALGVDPEVLA
                     GKLAEDGADFAYLARRVSGDAAKELERVIRDEGVNGLRIDRVSGRVYPERALAAHLVG
                     FVGTENKGLSGAEAA"
BASE COUNT          155 a          375 c          390 g          196 t
ORIGIN      
        1 ttgacggagg tcgagcccga taggtggcgc ggatcgcccc ggggccggct ctcagcggcg
       61 cgcatcatag tcgctgccgt ggcgctcgcg gtagcgctca aggcgttcgc cctggacctc
      121 gtcatagtca gcggcgagtc gatgagtccg accataggct ccggcgccgt ggccgtcgtc
      181 gcgccctgcg cctacggtct ccgcctaccg ctatctggat cgtacctggt ccgctgggga
      241 gagcctgccc cgggtgacat cgtgctcgtg tcgccgttcc cgcccgagcg tcgcaaggcc
      301 atcaagcgcg tgttcgacgt cggcccggcc ttcatcgtag cggaggccgg cagcctgcgc
      361 gggcgaggag gcgtcgtctc cctaggcccg tctccgcgct cgtcgctcgc cggcacggcg
      421 tacctgccgg caggcaaggc cttcgtcgtc ggcgacaacg cggaacgttc gatagactcg
      481 aggcgctacg gggctgtacc gattgagaaa ctggccggaa aggtgctact gtggttcggc
      541 ggagccgccg agccgacgtt acgaacgatt ttaagcaagg acgctgccga ggatgtcgat
      601 cgataagcgc tttatccgtc attacgtcat catcgccgcg ttgctggccg ttgccggctc
      661 gaccctggtg cgctacgccc gcctggccgg cggcgccggg gcggatcggc catcggcctc
      721 ggcctccaga gtcgtctcga tacgtggccc gatctacgac cgggagggcc gccttctcgc
      781 ggtcgatacc gacctctatg acgtctcgat ctggaagccg tccttccagg cggataaggt
      841 cggttcgttc gcgcgttcgg cctcggcagc gctcggcgtc gacccggaag tgcttgcggg
      901 caagctggcc gaagacgggg cggacttcgc gtacctggcc cggcgcgtct cgggcgacgc
      961 ggccaaggaa ctcgagcgcg tcatacgcga cgagggcgtg aacggcctcc ggatcgaccg
     1021 tgtatccggc cgggtgtatc cggaacgcgc gcttgcggcc caccttgtcg gcttcgtcgg
     1081 caccgagaac aagggcttgt ccggcgccga ggcggc
//
LOCUS       DAOPHV010000811         1115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_195354_length_1115_cov_4.217925, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000811 DAOPHV010000000
VERSION     DAOPHV010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195354_length_1115_cov_4.217925"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1115)
                     /locus_tag="PLQ29_14565"
     CDS             complement(<1..>1115)
                     /locus_tag="PLQ29_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPG87916.1"
                     /translation="QIGSLDSLHVPTLTVALSVVYVGSVLFSALLYIFKRRFRGDKLM
                     ILSQSLFAAAAFGLIAQDNGLSYEFLSLTNAAMLAAVLCIGHGLTRFGLMSRFSPWAY
                     AIVPLGVGGWFALGPFGVSARIVLFASAFTVVGALFAADVRRSSKDEFGSLRALFYLF
                     FIIVSVSSAGRGIRTVVWEPPRTIALDGSIGAASHLSALLIGFMNMFGYFLLSSAHAE
                     RQLGLREREIRKRNEELLETIETKDALISVIAHDLRAPVWSATRYVRSHLVDFDGDLN
                     VKRDSIVTLAEGLDRISGLLDSLLEWAMCASGRIKLQPVPVSLGEVLGEAIADLGPTA
                     ELKGVSIEAGLSDGLVVADRRALATALRNLLSNAIKYS"
BASE COUNT          245 a          350 c          349 g          171 t
ORIGIN      
        1 gaatacttga tcgcgttcga taataggttc cgaagcgcgg tcgcgagcgc ccgccggtcg
       61 gccacgacca agccatcgga taggccggcc tctatggaca cgcccttgag ctcggcagtc
      121 gggcctagat ccgcaatcgc ctcgccgagc acctccccga ggctgaccgg gaccggctga
      181 agcttgatgc gcccggaagc gcacatggcc cactcgagca gcgagtccag gaggccggaa
      241 atgcggtcca ggccctcggc caacgtgacg atgctgtcgc gcttaacgtt gagatcaccg
      301 tcgaagtcga cgagatggct ccgcacgtaa cgagtggcgc tccagaccgg agcgcgcagg
      361 tcgtgggcta tgacgctgat cagcgcgtct ttcgtctcga tggtctcgag cagctcctcg
      421 ttacgcttcc gtatctcccg ctcccgtaac cccagctgac gctcggcgtg ggcggacgac
      481 aggaggaagt acccgaacat attcatgaag cctatcagca acgccgacag gtgcgaggcc
      541 gcgcctatcg atccatcgag cgcgatcgtg cgcggcggtt cccagacgac agtccgaatg
      601 ccgcgacccg cgctcgaaac gctgacgatg ataaagaata ggtagaacaa cgcccgaagg
      661 ctaccgaatt cgtccttact ggaccgtcga acgtccgccg cgaagagcgc gccgacgacc
      721 gtgaaggccg aggcgaatag gacgatgcgg gcgctgacgc cgaacggtcc aagcgcgaac
      781 catccgccga cgcccagcgg cactatcgcg taggcccatg gcgagaatcg ggacatgagg
      841 ccgaagcgcg tcaagccgtg cccgatgcac agcacggcgg cgagcatggc cgcattggta
      901 agcgacagga actcgtacga caggccgtta tcctgggcta tcaacccaaa ggcggcggcc
      961 gcgaacaggg actgactgag tatcatcagc ttatcgccgc ggaaccttcg cttgaagata
     1021 tagagtaggg ccgaaaacag cacgctgccg acgtagacta ccgacagcgc gacggtaaga
     1081 gtcggtacgt gtagggaatc cagcgatcct atttg
//
LOCUS       DAOPHV010000812         1111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_196570_length_1111_cov_2.869318, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000812 DAOPHV010000000
VERSION     DAOPHV010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196570_length_1111_cov_2.869318"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PLQ29_14570"
     CDS             <1..548
                     /locus_tag="PLQ29_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPG87917.1"
                     /translation="RPYRSEVPAVRSFIQDVVGLASGWTMGVERVLFDLPTARSGNVR
                     FAAPICFEDAFAYLCRRFVLDGAEILVNLTNDSWSRTESAEIQHFVAARLRAVELRRT
                     LVRSTNGGVSAVVGPDGRVLDSLPLFVAEARLVKVPVYVGEETPYLRFGDWFAALLAC
                     ILASLAFILYFDDVASRKDSP"
     gene            545..919
                     /locus_tag="PLQ29_14575"
     CDS             545..919
                     /locus_tag="PLQ29_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87918.1"
                     /translation="MSVREYLSSPFYELQPYKSDPPQDALAFTGSPRKHPYDDGKIIL
                     LVEPNAGESAIYEFKAADIVGAQDLPSPVTEAGESYRIVRLWIRRGAVGIRYEPFEVE
                     APLRFMNDAKAFKDKMLGRHRG"
BASE COUNT          187 a          354 c          359 g          211 t
ORIGIN      
        1 cgaggccata ccgttctgag gttccggccg tccgctcgtt catccaagac gtcgtaggcc
       61 tggcgtcggg ctggacgatg ggcgtggaac gcgtgctgtt cgacctgccc acggcccggt
      121 cggggaacgt tcgcttcgca gctcccatct gcttcgagga tgccttcgcc tacctgtgcc
      181 gccgtttcgt cctcgacggg gcggagatcc tggttaatct gacgaacgac tcctggtcca
      241 ggaccgagtc ggcggagata cagcatttcg tggcggcgcg cctcagggcc gtcgagctca
      301 ggcggaccct cgtgcgctcg acgaacggag gcgtgtcggc ggtggtcggc cccgacgggc
      361 gggtgctgga ctctctgccg ctcttcgtgg ccgaagcccg cttggttaag gttccggtct
      421 acgttggcga ggagacgccc tacctgcgtt tcggcgactg gttcgcggcg ctcctggcct
      481 gtattctagc ctcattggcg tttatactat atttcgacga cgtagcgtcg aggaaggata
      541 gcccatgagc gtacgagaat acctgtccag cccgttctac gagctacaac cctataagag
      601 cgaccctcct caggatgcgc tcgccttcac cggctcaccg cgcaagcacc cctacgatga
      661 cggcaagatc atcctgctcg tcgaaccgaa tgccggcgag tcggccatct acgaattcaa
      721 ggcggctgac atcgtcggag cccaagacct gccgagcccc gtgaccgagg ccggggagag
      781 ctaccgcatc gtccgcctct ggatacgtcg cggggcagtc ggaatacgct atgagccgtt
      841 cgaggtcgag gccccgctac ggttcatgaa cgatgcgaag gcgttcaagg acaaaatgct
      901 cggccggcat agaggctaga gccgtggatc ctcgcgccgg gccggtggca cggtactatg
      961 aatgtctccc cgacggggac atccgttgcc ggctatgccc gcgccgctgc gtcatacgcg
     1021 acggcggcgc gggagcttgc cgggtcagga gcaactcagg cggaaccatg acgcttccgt
     1081 tctatggcat ggccagcgcc gtatcggccg a
//
LOCUS       DAOPHV010000813         1110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_196856_length_1110_cov_3.242654, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000813 DAOPHV010000000
VERSION     DAOPHV010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196856_length_1110_cov_3.242654"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PLQ29_14580"
     CDS             <1..538
                     /locus_tag="PLQ29_14580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPG87919.1"
                     /translation="RIHHLALNKPPGYICAMRDDYGRPLAASLFKPAIAERVYNVGRL
                     DLESCGLILFTNDGEFAARVGHPSSGVVKEYLVETDKPVPADFARRFVAGIEDDGETL
                     RALEATVTGERRCSIRLVEGRNREIRRALKGFGLRATLLRRVAIGDVRLGDLAEGAWR
                     SLSAKEVAGLMATMEGRR"
     gene            535..>1110
                     /gene="cmk"
                     /locus_tag="PLQ29_14585"
     CDS             535..>1110
                     /gene="cmk"
                     /locus_tag="PLQ29_14585"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPG87920.1"
                     /translation="MIVAMDGPAGTGKSTIARMVAERRGFTYINSGNLYRAITYGAMR
                     SGVPLDDEAALTSYAEGAGLEYRGSALYLDGEPIEGRLRSAEVDAVVAQVSAIVPIRH
                     IVNRLIRSIGAGLDAVVEGRDICTVVYPDAEVQIYIDASAEARARRRFDQGTSSLSLD
                     EIKKNIEMRDRIDMNKATFRLLHVYPIAHLYI"
BASE COUNT          204 a          349 c          375 g          182 t
ORIGIN      
        1 acgtatccac catctcgcgc tcaacaagcc gccgggctac atctgcgcca tgcgcgatga
       61 ctacggccgg ccgctcgccg cgtcgctgtt caagccggct atagccgagc gggtttataa
      121 cgtcgggcgg ctcgacctgg aatcgtgcgg gctcatcctg ttcacgaacg acggcgagtt
      181 cgccgcgagg gtaggccacc cgtcgtcggg cgtcgtcaag gagtacctcg tcgagaccga
      241 caagcccgtt ccggccgact tcgcgcggcg tttcgtcgcc gggatcgaag acgacggcga
      301 gaccctgcgg gcactcgagg cgaccgtcac gggcgagcgt cgctgctcga tcaggctcgt
      361 cgagggcagg aaccgcgaga tccggcgggc gctcaagggc ttcgggctca gggcgacgct
      421 cctacggcgc gtggccatcg gggacgtgcg gctcggcgac ctggccgagg gagcttggcg
      481 cagcttgagc gcgaaagagg tcgccggcct catggcgacc atggagggac gacgatgatc
      541 gtggcgatgg acgggccggc gggaaccggt aaaagcacga tagccagaat ggtggccgag
      601 cgccgaggct ttacctatat caactcgggc aacctgtatc gcgccataac ctacggcgcc
      661 atgcgctcgg gcgtgccgct cgacgacgag gccgccttga cgtcctatgc cgaaggcgcc
      721 ggactggaat accgcggcag cgctctctac ctggacggcg agccgatcga gggccggcta
      781 cggagcgccg aggtggacgc ggtcgtggcc caggtctcgg cgatcgtgcc catacggcat
      841 atcgtcaacc ggctgatccg ctccattggc gccggcttgg acgccgtcgt cgagggccgg
      901 gacatctgca ccgtcgtcta tccggacgcc gaggtgcaga tctacatcga cgccagcgcc
      961 gaagccaggg ccaggaggcg cttcgaccag ggtacgagct cgctaagtct cgatgagatc
     1021 aagaagaata tagagatgcg cgataggata gacatgaata aggctacctt ccgccttctt
     1081 catgtctatc ctatagcgca tctctatatt
//
LOCUS       DAOPHV010000814         1109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_197098_length_1109_cov_4.740038, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000814 DAOPHV010000000
VERSION     DAOPHV010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197098_length_1109_cov_4.740038"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1109)
                     /locus_tag="PLQ29_14590"
     CDS             complement(<1..>1109)
                     /locus_tag="PLQ29_14590"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87921.1"
                     /translation="ADSPERLDAFCYVSASHNPPGHNGVKFGLSDGGVLPGPEAKALI
                     ASLKDGACTAADIAAMAALSAAAEPRAVAALYSGCATAKRRAISDYTLFAREVAGASP
                     ELAEQERELDALEAAIAARGAGVVAEMNGSARSLSIDGDYLASIGVATLRLNDEPRRF
                     AHRIVPEGESLEPCRLALAEARRADPRFSLGYVPDCDGDRGNIVYWDEADGEAKALEA
                     QDTFALAALAELSCSAARAFARGESPRLAVACNDATSLRVEALASALGAQAFRAETGE
                     ANVVGLARSLRERGYEVRVLGEGSNGGVITHPASVRDPLNTMTALLKLLYLRDDGDAP
                     GPFRLWLERSGQGEAYREDFGLADVLASLPRFSTT"
BASE COUNT          144 a          397 c          419 g          149 t
ORIGIN      
        1 ctcgtcgtcg agaagcgcgg caggctcgct agcacgtcgg ccaggccgaa gtcctcgcgg
       61 taggcctcgc cctgcccgga ccgttcgagc cacagcctga aggggccggg cgcgtcgccg
      121 tcgtcgcgta ggtacagcag cttgagcagg gcggtcatcg tgttgagcgg gtcgcgcacg
      181 ctcgccggat gggtgatgac gccgccgttc gagccctcgc cgaggacgcg gacctcgtag
      241 ccgcgctcgc gcagggaacg ggccaggccg acgacgttcg cctcgccggt ctcggcccgg
      301 aacgcctgcg cgccgagggc ggaggccagc gcctcgacgc gcaggctggt ggcgtcgttg
      361 caggcgaccg cgagccgcgg cgactcgccc ctggcgaagg cgcgggcggc cgagcaggac
      421 agctcggcga gcgcggccaa cgcgaaggtg tcctgggcct cgagcgcctt ggcctcgccg
      481 tcggcctcgt cccagtagac gatgttgccg cggtcgccgt cgcagtccgg cacgtagccg
      541 agcgagaagc gcgggtcggc ccgcctcgcc tcggccagcg ccaggcggca cggctcgagc
      601 gactcgccct ccggcacgat gcggtgggcg aagcggcgcg gttcgtcgtt caggcgcagg
      661 gtggcgacgc ctatcgacgc caggtaatcg ccgtctatcg acagcgaccg ggccgagccg
      721 ttcatctcgg ccaccacgcc ggcgcccctg gccgctatcg cggcctcgag cgcgtccagt
      781 tcgcgttcct gctcggcgag ttccggagag gctccggcca cctcgcgggc gaacaaggtg
      841 tagtccgaga tcgcgcggcg cttggccgtg gcgcagccgg agtacagcgc ggccaccgct
      901 cgcggctcgg cggcggcgct cagcgccgcc atcgcggcta tatcggccgc cgtgcaggcg
      961 ccgtccttga gcgaagctat cagggccttg gcctccgggc cgggcagcac gccgccgtcg
     1021 gacaggccga acttgacgcc gttatggccg ggcgggttat ggctcgcgga gacgtagcag
     1081 aacgcgtcga gccgctcggg gctgtcagc
//
LOCUS       DAOPHV010000815         1107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_197679_length_1107_cov_4.553232, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000815 DAOPHV010000000
VERSION     DAOPHV010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197679_length_1107_cov_4.553232"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..>1107
                     /locus_tag="PLQ29_14595"
     CDS             184..>1107
                     /locus_tag="PLQ29_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87922.1"
                     /translation="MTRRSKDTIRKAAIVAAGVAMIASLAILTSARIQSRFVESQSGS
                     ALVFIDKYVDEPLRAEFTAGIDSYTGGFLKAARLLGEAPIRGPVAFFAADRHYGVISA
                     NDPLVKRRLSGRPSSDFVLFRSIGSNFYANELVARSIASQDGAYVFVNIDEGWRASLV
                     HAYAHSIAAANAPAALSPAFVLDQDFDSEMKFSYRFADETFALLASDLLELAEDLGSV
                     EQAWSSFSAATARRYADPESDVAAREAEIIMATYDLPQKSAEFYAACNGFASWLLDAY
                     GRDALCAAARTFFSGRYDGLDDPFIAFGGLEK"
BASE COUNT          188 a          374 c          349 g          196 t
ORIGIN      
        1 tcgctcatga tacgctcctt tttcgccagg ccgagggtga agcgcggctt ctcgcggccc
       61 gacgccgcgt attatgcgac gaatggacgg aatggtcaaa tacgccgggc cgacgccgtc
      121 gaagctcgac gccgcttgcg cggcccacgg cgctggggat agtatcgccc gagaggttcg
      181 aacatgacga gacggagcaa ggatacgata cggaaggcgg cgatcgtggc ggcaggcgtc
      241 gccatgatcg cctcgcttgc catcctgacc tcggcgcgga tacagagccg tttcgtggag
      301 agccagtccg gaagcgcgct tgtattcatc gacaagtacg tcgacgagcc tctgcgcgca
      361 gaattcacgg cgggcatcga ctcctatacc ggaggcttcc tgaaagccgc acgcctgctc
      421 ggcgaagccc cgatacgcgg gccggtggcc ttcttcgccg ccgatcggca ctacggcgtc
      481 atcagcgcca acgacccgct cgtcaagcgg cgcctcagcg ggcggccgtc gtcggacttc
      541 gtgctcttca ggtcgatagg atccaatttc tacgccaacg agctggtggc gaggtcgata
      601 gcgtcccagg acggcgccta cgtcttcgtc aatatcgacg aaggctggcg ggcctcgctc
      661 gtccacgcct acgcccactc gatagccgct gccaatgcgc cggccgccct gtcgccggca
      721 ttcgtcctgg atcaggactt cgattccgag atgaaattct cgtaccgctt cgccgacgag
      781 accttcgccc tgctcgcgag cgacctcctc gagctggccg aggacctggg gagcgtcgag
      841 caggcctggt cgtcgttctc ggcggccacg gcgaggcgct acgccgaccc cgaatccgac
      901 gtggcggccc gcgaggccga gatcatcatg gctacctatg acttgccgca gaaatcggcg
      961 gagttctacg ccgcttgcaa cggtttcgcc tcatggctcc tcgacgccta cggccgcgac
     1021 gcgctatgcg ccgccgccag gaccttcttc tcgggacgct acgacggcct cgacgatccg
     1081 ttcatagcct tcgggggcct ggagaag
//
LOCUS       DAOPHV010000816         1107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_197742_length_1107_cov_2.799430, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000816 DAOPHV010000000
VERSION     DAOPHV010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197742_length_1107_cov_2.799430"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..906
                     /locus_tag="PLQ29_14600"
     CDS             346..906
                     /locus_tag="PLQ29_14600"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPG87923.1"
                     /translation="MRALYPWIELSSKSVLDCRVFKVSSSERQSTKGKRGTFFSVDAP
                     DWAGVIPIVETPEGRSFVMIRQFRHGTRHLSIEFPGGIVEPGEDPATAIARELIEETG
                     YRAEMIVPLGILSPNPAFMTNTFHAFVAEGCVLERAQCLDEHEEIEVLLVPEREAIDM
                     IGAEDMGHALMTATLFFYERYRGKLD"
     gene            complement(968..1099)
                     /locus_tag="PLQ29_14605"
     CDS             complement(968..1099)
                     /locus_tag="PLQ29_14605"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPG87924.1"
                     /translation="MALRRYGEPDEFGRTAAWLLSPASSYLTGQLVTVDGGSLRGSW"
BASE COUNT          202 a          363 c          362 g          180 t
ORIGIN      
        1 cacgtcgtgc tcgtgcgtcc agccgaggtc cccggggacg ccgatataga tgaaggctac
       61 cttgtacggc tcggcgacga ccggctcggc gaccggctcg gccttcttgg cgcatccgaa
      121 catggtcagc gccgcgagca gggcgctcgc gatgatcaac gtctttttca tcagttctct
      181 cctttaaaca gcgatgcgtg atagccgtct agtcgggaaa agctcgaagc gggacgacgg
      241 tgcaatcaat aaacaacgcg agccgtggct tgtcaaatcg tttcttcgcg acggcacgat
      301 cgcggcgggc ttgccggccg gtcgcggaag aattagtatt cctgtatgcg cgcgctatac
      361 ccgtggatag agctatcgtc caagagcgtc ctcgactgca gggtgttcaa ggtcagctcg
      421 agcgagcggc agtctaccaa gggtaagcgc ggcaccttct tctcggtcga cgcgcccgac
      481 tgggcgggcg tcatacccat cgtcgagacc cccgagggcc gctccttcgt gatgatacgg
      541 cagttcaggc acggcacgag gcacctgtca atcgagttcc cggggggtat cgtcgagccg
      601 ggcgaggacc cggcgacggc gatagccagg gagctgatcg aggagacggg ctaccgcgcc
      661 gagatgatcg tgccgctcgg catcctgtcc ccgaacccgg ccttcatgac caataccttc
      721 cacgccttcg tcgccgaggg atgcgtcctc gagcgggccc agtgcctcga cgagcacgag
      781 gagatcgagg tgctcctcgt gcccgagaga gaggccatcg acatgatagg cgcggaggac
      841 atgggccacg cgctgatgac ggccaccctg ttcttctacg agcgctaccg cggcaagctc
      901 gactagccgg agccgcccgg agccgctcag agccgcccgg agccgcccgg agtcgcccgg
      961 agccgcccta ccaagagccg cgcaggctgc ccccgtcgac ggtgacgagc tggccggtca
     1021 ggtacgacga cgccggtgac aggagccacg ccgccgtacg accgaattcg tccggctcgc
     1081 cataacgccg gagcgcgatg gccgcct
//
LOCUS       DAOPHV010000817         1105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_198340_length_1105_cov_2.624762, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000817 DAOPHV010000000
VERSION     DAOPHV010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198340_length_1105_cov_2.624762"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLQ29_14610"
     CDS             complement(<1..500)
                     /locus_tag="PLQ29_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87925.1"
                     /translation="MNDRTDDARARFRHEARTILNHVVGFGELLSGDADLTTDPAKGK
                     ALADIGKAAAALRQPVLEYVGSVIDGSGPSDALRRQVYERIYDLVSLVQAAKRTSSRG
                     DRFIGDVRKVHEAVNALAALFDEAGPASGKDADRAPAGGRSLEPAKAGRSGRILVVDD
                     DSFNRE"
     gene            complement(513..647)
                     /locus_tag="PLQ29_14615"
     CDS             complement(513..647)
                     /locus_tag="PLQ29_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87926.1"
                     /translation="MTYKLDGAKFQTLDELVEALYPMYADEMSEEEFRKYAEEHAEKG
                     "
     gene            774..>1105
                     /locus_tag="PLQ29_14620"
     CDS             774..>1105
                     /locus_tag="PLQ29_14620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPG87927.1"
                     /translation="MDRTRIIKRAAVVSLVGNAALAVLKVAAGFAAGSLAVVGDGVDS
                     STDVLISIVALVAAAVSSKPSDKEHPYGHARAETTATTVLAFFIFFAGAQLILATVRS
                     LLAGEARSV"
BASE COUNT          161 a          381 c          345 g          218 t
ORIGIN      
        1 atctcgcggt tgaacgagtc gtcatcgacg accaggattc ggcctgaacg cccggccttg
       61 gcgggttcga ggctcctgcc gccggccggc gccctgtcgg cgtccttgcc cgaggccggg
      121 ccggcttcgt cgaagagggc ggccagcgcg ttgaccgcct cgtgtacctt acgtacgtcg
      181 cctatgaagc ggtctcccct cgacgaggtc cgcttggccg cctggacaag cgagacgagg
      241 tcgtagatac gctcgtagac ctgcctacgg agcgcgtcgg agggcccgga gccgtcgatg
      301 acggaaccga cgtattccag gaccggctgc ctgagggccg cggcggcctt gccgatgtcg
      361 gctagggcct tgcccttggc cggatccgtg gtcaggtcgg catcgccgga tagaagctcc
      421 ccgaagccga cgacgtggtt gaggatggtt cgcgcctcgt ggcggaaacg ggcccgcgcg
      481 tcgtcggtcc tgtcgttcat cgcctcttcg tcctatccct tctcggcatg ctcttcggca
      541 tacttccgga attcctcttc cgacatctcg tcggcgtaca tcggatacag cgcctcgacg
      601 agctcatcta gcgtctggaa ttttgctccg tcaagtttat atgtcatcta cggctccctg
      661 cgcccggcgt cgggcgagcg tgcatcctag cctaaggtaa cgcatttagc aaggaagtaa
      721 cgcttccatg gaaggttctt gcccgccgcg acgccgcggc ctatactcgc ccgatggata
      781 gaacccggat catcaaacgc gccgccgtcg tcagcctcgt cggtaacgcg gcgctggctg
      841 tgcttaaggt cgcggccggc ttcgcggccg gctcgctcgc cgtcgtgggc gatggcgtcg
      901 attcgtcgac cgacgtgctg atctctatcg tcgccctggt cgcggccgcc gtcagctcca
      961 aaccgtccga caaagagcac ccctacggac acgccagggc cgagaccacg gccaccacgg
     1021 tcctcgcctt cttcatcttc ttcgccgggg cccagctcat cctcgctacg gtgcgctcgc
     1081 tgctggccgg cgaagcccgg tccgt
//
LOCUS       DAOPHV010000818         1094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_201433_length_1094_cov_4.003850, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000818 DAOPHV010000000
VERSION     DAOPHV010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201433_length_1094_cov_4.003850"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLQ29_14625"
     CDS             <1..266
                     /locus_tag="PLQ29_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87928.1"
                     /translation="PFAAYYLSVEKAGEGGKGLLEVFGPGAKPVIPADCLGALGPAVE
                     AAAAKTAAIIERGSIYVPAVRDRDDVCEGCELRPVCRARYAVR"
     gene            269..>1094
                     /locus_tag="PLQ29_14630"
     CDS             269..>1094
                     /locus_tag="PLQ29_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPG87929.1"
                     /translation="MGAWNDIKAGLDPARQLPAVEETRSAVVSAGAGSGKTRVLAVRY
                     LHLVKERGIPPERILCMTFTKKAAAEMSERIRAMLAACAVDDDDFKKALEAFPSSRVS
                     TLDSFCSEVARDGCARWGVPPDFAVDKDPGDLGALAIEFLLERRDDPVAASFIAASGF
                     EKAAEALAALAGGREGLIATDRPFDPDSQAAALGALLADAHGQALELLRGGLALDDAG
                     VKGAMAWLEAAGAFAPEPPAANDAEALGAAAAAYRALAGLRLSTGKAEAAVYYNEAG"
BASE COUNT          151 a          389 c          404 g          150 t
ORIGIN      
        1 agcccttcgc ggcgtactac ctgtccgtcg aaaaggccgg cgagggcggc aaggggctgc
       61 tcgaggtctt cggcccgggt gccaaaccgg tcatccccgc cgactgcctc ggtgcgctcg
      121 ggccggccgt ggaagcggcg gccgccaaga ccgcggccat catcgagcgg ggctcgatct
      181 acgtcccggc cgtgcgcgac cgggacgacg tctgcgaagg gtgcgagctg cggccggtct
      241 gccgcgcccg ctacgcggtg aggtaggcat gggagcctgg aacgatatca aggcggggct
      301 cgaccccgcg cgccagctac cggcggtcga ggagacccga tcagccgtcg tctcggccgg
      361 cgccggctcc ggcaagacgc gcgtgctcgc ggtgcgctac ctgcatctgg tcaaggagcg
      421 cgggatcccg ccggagcgca tactctgcat gaccttcacc aagaaggcgg ccgcggagat
      481 gagcgagcgc atccgcgcga tgctggccgc ctgcgcggtc gacgacgacg atttcaagaa
      541 ggcgctcgag gccttcccct cgtcgcgcgt gtcgacgctc gactcgttct gctccgaggt
      601 agcgcgcgac ggctgcgccc gctggggcgt gccgccggat ttcgcggtgg acaaggatcc
      661 cggcgaccta ggcgctctgg cgatagaatt cctactcgag cggcgcgacg atccggtcgc
      721 ggcctcgttc attgcggctt ccggcttcga gaaggccgcc gaggcgctcg ccgccttggc
      781 gggaggccgc gaggggctca tcgccacgga ccgacccttc gaccccgata gccaagcggc
      841 ggccctcggc gccctcctgg ctgacgccca cggtcaggcg ctcgagctct tgcgcggcgg
      901 gctcgctctg gacgacgccg gggtcaaggg agcgatggcc tggctcgagg cggcgggggc
      961 cttcgcgccc gagccgccgg ccgcgaacga cgccgaggcg ctcggggctg ccgcagcagc
     1021 ctatcgcgcg ctcgccgggc tacgcctctc gaccggcaag gccgaggcgg ccgtctacta
     1081 caacgaggcg ggca
//
LOCUS       DAOPHV010000819         1091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_202494_length_1091_cov_2.552124, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000819 DAOPHV010000000
VERSION     DAOPHV010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202494_length_1091_cov_2.552124"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLQ29_14635"
     CDS             complement(<1..228)
                     /locus_tag="PLQ29_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87930.1"
                     /translation="MEHRLERRRPFVLVGMNFFGNPFGAAAAWDMDNEIGSLWKRLMA
                     YLADHPVAALPPDDPAWFEAHIMHPASAATGD"
     gene            450..923
                     /locus_tag="PLQ29_14640"
     CDS             450..923
                     /locus_tag="PLQ29_14640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmO family methyltransferase"
                     /protein_id="HPG87931.1"
                     /translation="MKEMTVRAIGRVRADDAKGYYALEVDEPYREGLLALGEFGRLNA
                     LWWAHGMDTLEFRGTLSCELPYAPGVRAGVFSCRSEYRPNPIALTSCGIGSVDVDTGL
                     ITLDYIDAVDGTPLLDIKPYIPVCDRARDVRVAPWFREWPDCIEDSAAFFAKFEL"
BASE COUNT          160 a          350 c          391 g          190 t
ORIGIN      
        1 gtcgccggtc gccgcgctgg ccggatgcat gatgtgggcc tcgaaccagg ccgggtcgtc
       61 gggcggcaac gccgccacgg gatggtcggc caggtaggcc atcaggcgct tccagagcga
      121 gcctatctcg ttgtccatgt cccaggccgc ggccgcgccg aacgggttgc cgaagaaatt
      181 catgccgacg agtacgaagg gcctccggcg ctcgagcctg tgttccatcc cggcctccat
      241 acgcgacgat agcgccggag tgcccgcctg gcaaggccgc gggcgctccc cggctactga
      301 tagggcaatt ccggtcggtt tcgacctctg tccctctgga gctcggcctg gtgtcgtcct
      361 cgcgccgcgg cgtccggcaa tcgaggtcga gaggcgcgcg ccgccgaggc gctacgctga
      421 ggccatcacg acaccttatg gaggcgacga tgaaggagat gacggtacgg gcgatcggcc
      481 gggtgcgggc ggacgacgcg aagggctact acgcgctcga ggtggacgag ccgtatcgcg
      541 agggactcct ggccctgggg gagttcgggc gcctgaacgc gctatggtgg gcgcacggca
      601 tggatacgct agagttccgc ggcacgctgt catgtgagct gccgtacgcg ccgggcgtca
      661 gggccggagt gttctcgtgc cgttcggagt accggcccaa cccgatagcg ctgacgagct
      721 gcgggatcgg gtcggtcgac gtcgatacgg gcctgataac gctcgactac atcgacgccg
      781 tggatggtac gccgctcctc gacatcaagc cctacatacc ggtttgcgac cgcgccaggg
      841 atgtccgtgt ggcgccatgg ttcagggagt ggcctgactg catcgaggac tccgcggcgt
      901 ttttcgcgaa gttcgagctc tagcggcgaa tcgttcggcg cgggggcctc cgaggctccc
      961 gcgccgtgcg cgttgggcgc gttacgcgcc gggtatgatc acctcgccgt cgccctggta
     1021 gcgcctcgtg ctcgtccagt cgttcgtcgg cagccatagc gcggcccatt cgtagttatc
     1081 caggtcgacg g
//
LOCUS       DAOPHV010000820         1089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_203067_length_1089_cov_2.625725, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000820 DAOPHV010000000
VERSION     DAOPHV010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203067_length_1089_cov_2.625725"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..>1089
                     /locus_tag="PLQ29_14645"
     CDS             106..>1089
                     /locus_tag="PLQ29_14645"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPG87932.1"
                     /translation="MLRDDTGIIFNRELVPGFRLLADEMPGATGVAIGLWFPVGSALE
                     RDGERGLSHFVEHMVFKGAGDRDAAELSRSIDRVGGYLNAFTEREAVCLHCLVPARHA
                     ELAVDILMDMAYRPRLSAVEFEREKDVIVNEILAAEDDLEEAAQDEFFALAYDGHPAA
                     RRIAGEVGDIREAVFSSLAEFHDERFSRGPVTLTVSGAVQAEALAERVVAALPAQRRS
                     ASGLAEAWDPGEPRFVRCRRMVKAEGSQVYLFTGLPIEPTISEDDFWRLSVASMAYGE
                     SMSSRLFMRLREERGLCYGISSAFSLSRYAGLWGVSSSTTPAQLSRFSEAYL"
BASE COUNT          170 a          337 c          379 g          203 t
ORIGIN      
        1 cgacgcgggc cagaagaagt aggatacatc gggccagtcc cgagcgcgta tagaaggccg
       61 gccgcgagtc cggccttctt cattcataag acgcgaggat cgcagatgct ccgtgacgat
      121 actggaatca tctttaaccg cgagctggtc cccggcttca ggcttctggc cgatgagatg
      181 ccgggggcga cgggcgtagc catcggcctt tggttccccg tcggctcggc cctcgagcgg
      241 gacggggagc gtgggctgtc gcacttcgtc gagcacatgg tgttcaaggg cgcgggcgac
      301 cgggacgcag ccgagctttc gcgctcgata gaccgggtcg gtggctacct gaacgccttc
      361 accgagcgcg aagccgtctg tctgcactgc ctggtgccgg ccaggcatgc ggagctggcc
      421 gtcgatatac tcatggacat ggcctatagg ccgcggctgt cggctgtaga attcgagcgc
      481 gagaaggacg tcatcgtcaa cgagatcctg gctgccgagg acgacctcga ggaggctgcc
      541 caggacgagt tcttcgcgtt ggcctacgac ggacatccgg ctgctaggcg catagccggc
      601 gaggtcggcg atatccgcga ggcggttttc tcctcgctgg ccgagttcca cgacgagcgc
      661 ttctccaggg ggccggtaac gttgacggtc tccggggccg ttcaggccga ggccttggcc
      721 gaacgcgtcg ttgcggcgtt accggcgcag aggcgctcgg cgagcggcct ggcggaagcc
      781 tgggatcctg gcgagcctcg gttcgtccgc tgccggcgca tggtcaaggc cgagggtagc
      841 caagtctact tgttcaccgg cctgcctatc gaaccgacca tttcggagga cgacttctgg
      901 cgcctgtcgg tcgccagtat ggcctatggc gagagcatga gctcgcggct cttcatgcgg
      961 ctgcgcgagg agcgcggcct gtgttacggc atatcgtcgg cgttcagcct cagccggtac
     1021 gccggccttt ggggcgtctc gtcatccacg acgccggccc agctcagccg tttctcggaa
     1081 gcgtacctg
//
LOCUS       DAOPHV010000821         1087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_203719_length_1087_cov_2.270349, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000821 DAOPHV010000000
VERSION     DAOPHV010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203719_length_1087_cov_2.270349"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="PLQ29_14650"
     CDS             complement(<1..736)
                     /locus_tag="PLQ29_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87933.1"
                     /translation="MRRFSFTLTVLALLGVAVFMIGWIPLRLRPGSYAVLVTKTGGVD
                     PKVVAPGHFRWAAEALLPTNLVLRRFSPAAAERRLEASGELPSAEVYGAFMAGEPDFS
                     YSLVASLIAAPKPEALPELYERWGVTDDEALAAWLASELDLATADLRAAIQEALRSGS
                     SSPLGGVDERALALALSQSHPLLDLRGVRIVSSRMPDARLYEEARRLYALYLETYRAA
                     VEPALAESSAGAAVENVRLERLERYGE"
     gene            853..>1087
                     /locus_tag="PLQ29_14655"
     CDS             853..>1087
                     /locus_tag="PLQ29_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPG87934.1"
                     /translation="MNDDRALTVSELTGIVKELLEGSLPSVLVEGEISNWRPASSGHV
                     YFTLKDAGASLQAVMFKGRASRLDFKPSDGALVR"
BASE COUNT          186 a          361 c          387 g          153 t
ORIGIN      
        1 gctcaccgta gcgctcgagc cgctcgagcc tgacgttctc gaccgcggct ccggccgacg
       61 actcggcgag ggccggctcg acggcggctc gataggtctc gaggtacagc gcgtacagcc
      121 gcctggcctc ctcgtacagc cgcgcgtcgg gcatgcgcga cgatacgata cggacgcctc
      181 gcaggtcgag gagcggatgg gactgagaca gggccagggc cagcgcgcgc tcgtcgacgc
      241 cgccgagtgg agacgacgat ccggagcgca gggcctcctg gatggcggcg cgcaggtcgg
      301 ctgtcgccag gtcgagctcg gaggcgagcc aggcggccag agcctcgtcg tcggtgacgc
      361 cccagcgctc gtacagttcg ggcagggcct ccggcttagg cgccgctatc aggctggcga
      421 cgagcgaata cgagaaatcc ggctcgccgg ccatgaaagc gccgtagacc tcggctgagg
      481 gtagctcgcc ggacgcctcg agcctacgct cggccgcggc cggggagaag cggcgcagga
      541 ccaggttggt cggcagcagc gcctcggccg cccagcggaa gtgtccgggt gcgaccacct
      601 tcgggtcgac gccgccggtc ttggtgacca gcaccgcgta gctacccggc cggagccgta
      661 gcggtatcca cccgatcatg aagacggcta cgccgagcag ggccaggacg gtcaacgtga
      721 acgagaaacg tctcatgcca tccgttatac aggatattcg acccttttgc cactggacgt
      781 gcgccgccgc accggacgtg cgcgacagtg ccggacttgc gccgccgagc cgggccgggc
      841 taaagtgtcg gcatgaacga cgaccgagcg ctgaccgtga gcgagctcac cggcatcgtc
      901 aaggaactcc tcgagggctc gctaccgagc gtactggtcg agggcgagat atcgaactgg
      961 cgacccgcct catccggcca cgtctatttt acgctgaagg acgcaggcgc gtcgctccag
     1021 gcggtgatgt tcaaggggcg cgcgtccagg ctcgacttca agccatcgga cggcgccttg
     1081 gtcaggg
//
LOCUS       DAOPHV010000822         1086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_203868_length_1086_cov_4.546072, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000822 DAOPHV010000000
VERSION     DAOPHV010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203868_length_1086_cov_4.546072"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1086
                     /locus_tag="PLQ29_14660"
     CDS             <1..>1086
                     /locus_tag="PLQ29_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPG87935.1"
                     /translation="DYDDYDDYDDYDYDDDGIDNAGIDNAGIDNTGIDNTGIDNMGID
                     NAGDGAEDYAEDYAWDEPGQVDTLGAYDDLAALGANGPLDDVPGAIGEALKAGLEELT
                     GLSLALTVQPDRAWLRFGVELAAGGTLAELAEAASSGDRSLPYLSYCDADALISFAWS
                     APYDWSLPLLEGLYALILPDGSFADDAIAAAKAYAAASGLNGGMSISLAPSAELVRAV
                     RTNADLDDAEALELVSRGLGLSVSGAMELRDRQAFRDAAAEAFGAIAAPEYAELMEAS
                     GLSMSVDRGVGVASGMPYDAYRYEFTPTEAEEADSGEAMAMRLIGRLMAPVMVYRDDK
                     AFIGLGDPKAVASGIAKDGAASPLRAD"
BASE COUNT          185 a          361 c          387 g          153 t
ORIGIN      
        1 acgattatga cgattacgac gattacgacg actacgatta cgacgacgac ggcatcgaca
       61 acgcaggcat cgacaacgca ggcatcgaca acacgggaat cgacaacacg ggcatcgaca
      121 acatgggcat cgacaacgca ggcgacgggg ccgaggacta cgccgaggac tacgcgtggg
      181 acgagccggg ccaggtcgac actctaggag cgtacgacga cctggcggcg ctcggcgcta
      241 acggcccgct cgacgacgtg ccgggggcga taggcgaggc gctgaaggcg ggcctggaag
      301 agctgaccgg actctcgctt gcgttgaccg tccagcccga ccgggcctgg ctccgcttcg
      361 gcgtggagct ggccgccggc ggaacgctgg ccgagctagc cgaggccgcg tcctcgggcg
      421 accgctccct accctacctg agctactgcg acgccgatgc gctcatatcc ttcgcgtggt
      481 cggccccgta cgattggagc ctgccgctgc tcgaggggct ctacgcgctg atcctgcccg
      541 acggctcgtt cgccgacgac gcgatcgcgg cggccaaggc ctacgccgcg gcgagcggcc
      601 tgaatggcgg gatgtcgata tccctggcac cgtcggccga actggttcgg gctgtccgga
      661 cgaacgccga cctggacgac gccgaggcgc tcgagctggt gtcgcgcggc ctcgggctgt
      721 cggtctccgg ggcgatggag ctacgcgaca ggcaggcttt ccgcgacgcc gcggccgagg
      781 ccttcggcgc catagccgcg cccgagtacg cggaactgat ggaagcgtcc gggctttcga
      841 tgtcggtgga ccgcggcgtc ggcgtggcct cgggcatgcc ctacgacgcc taccgctacg
      901 agttcacccc gacggaggcc gaggaggccg attcgggcga ggccatggcg atgaggctga
      961 tcggcaggct gatggccccg gtcatggtct atcgcgatga caaggccttc atcggcctgg
     1021 gggatcccaa ggccgtggcc tccgggatag ccaaagacgg cgccgcgagc ccgctacgcg
     1081 cggaca
//
LOCUS       DAOPHV010000823         1084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_204497_length_1084_cov_3.689990, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000823 DAOPHV010000000
VERSION     DAOPHV010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204497_length_1084_cov_3.689990"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLQ29_14665"
     CDS             complement(<1..405)
                     /locus_tag="PLQ29_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87936.1"
                     /translation="MPKLATFIEKEFILNQVQKSGSRVVVIGSGKSVDGRLKAFDKET
                     LSLSATASAAEAFKSWDPVSVFLSYQSQRVTFPGKVKRVAGGEIVIGLPENLVKAPQR
                     KAVRVPPPKDLRLEFYLQNERIRIDCPESEEYS"
     gene            599..>1084
                     /gene="pgi"
                     /locus_tag="PLQ29_14670"
     CDS             599..>1084
                     /gene="pgi"
                     /locus_tag="PLQ29_14670"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPG87937.1"
                     /translation="MERWKRIDELAGWRELVRRRPEILRGALTPGRLASRTLPAGGGL
                     SYSYAAMDADDGTIASLAAAATELGLVDQYRALASGEVMNVGEGRMVLHHLARGTPCG
                     PVVKGGRDYRAFYEGERSRAAAFSAMVRSGELRGSTGKRFERVVQIGIGGSDLGPRAL
                     YL"
BASE COUNT          189 a          335 c          361 g          199 t
ORIGIN      
        1 tgagtattcc tcagactcgg ggcaatcgat gcggatacgt tcgttctgga ggtagaattc
       61 gagcctgagg tctttgggag gaggcaccct gaccgccttg cgctgcgggg ccttgacgag
      121 gttctcgggc aggccgatga cgatctcgcc gccggcgacg cgcttgacct tgccggggaa
      181 ggtgactcgc tggctctggt acgataggaa gacggagact gggtcccagg atttgaacgc
      241 ctcggctgcg gacgctgtgg cgctcaggct gagcgtctcc ttatcgaagg ccttgagccg
      301 gccgtcgacg ctcttgcccg agcctatgac caccaccctg ctaccggact tctgcacctg
      361 gttgagtatg aattcttttt ctatgaacgt cgctagcttc ggcatcggca tcccctgcga
      421 ttaggtcgtt cgattgcgtg tcgacgacgc cagtatacca gaaactccgg caaccgtata
      481 cgaatccccg gctcgctttc tatattgaaa aaaggtgccg ctaagcatag tataagcgta
      541 tcgaaatgaa acactcgtat ccggccagaa gccaaggtcc cggatgaaag gatacccgat
      601 ggaacgttgg aagcgtatcg acgagctcgc cggctggcgg gagctggtga ggcgacggcc
      661 agagatcctg cgcggagccc taaccccggg gcgcctcgcg tccaggacgc tgccagccgg
      721 cgggggcctc agctactcct acgcggcgat ggacgccgac gacgggacga tagcctcgct
      781 ggcggcggcg gcgaccgagc tcggcctggt cgaccagtac cgggccctgg cctccggcga
      841 ggtgatgaac gtcggcgaag gccgcatggt gctgcaccac ctggcccgcg ggactccctg
      901 cggccccgtc gtcaagggcg gccgcgacta tcgggccttc tacgagggag agcgctctcg
      961 cgccgcggcc ttctccgcga tggtccgttc cggggagcta cgcggctcga ccggcaagcg
     1021 cttcgagcgc gtcgtgcaga taggcatagg cggctccgac ctggggccgc gagcgctgta
     1081 cctg
//
LOCUS       DAOPHV010000824         1082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_205161_length_1082_cov_4.103213, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000824 DAOPHV010000000
VERSION     DAOPHV010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205161_length_1082_cov_4.103213"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PLQ29_14675"
     CDS             complement(<1..342)
                     /locus_tag="PLQ29_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87938.1"
                     /translation="MRRMLLAASAACILGASCASLGDPFLVAFGVDSAYQSEALTASG
                     IDEYRERLLGQGDVAAAASIQRLFEAALRYDPNNAEAARYLALVEDFRASRFSSSMAK
                     AEGLLAKPKRNP"
     gene            604..>1082
                     /locus_tag="PLQ29_14680"
     CDS             604..>1082
                     /locus_tag="PLQ29_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HPG87939.1"
                     /translation="MEKGKTMDAALAKKVSGFQRDEITGCILYGKLSRVIKDANNAKV
                     LADMAQAERQHYEFWKSLSGKDEAPYRGLVFFYYWCARILGLTFALKLMEKGEKAAID
                     GYRAVAHLVPQAERIGREEEEHEDSLLAMLDEERLAYMGSIVLGLNDALVELTGTLAG
                     "
BASE COUNT          224 a          334 c          353 g          171 t
ORIGIN      
        1 ggggttgcgc ttaggcttgg cgagcaggcc ctccgccttg gccatcgagc tggagaagcg
       61 actggccctg aaatcctcga cgagggccag gtacctcgcg gcctcggcgt tgttcggatc
      121 gtagcgcaac gcggcctcga acaggcgctg gatcgacgcc gcggccgcca cgtcgccctg
      181 gcctaagagg cgctctcggt actcgtcgat gcccgacgcc gtcagggcct ccgattggta
      241 cgccgaatcg acgccgaacg cgacgaggaa ggggtcgccg agcgacgcgc acgaggcgcc
      301 cagaatacag gccgccgagg cggctagcaa cattctacgc atggctcact ccgggtagcg
      361 catgagcgct acgacacacg atatagtatc gtgcatggga agcctttcca acagccccgg
      421 accgatacac ttcgtagcgc cgcggcttcc attccactcg aggccagaat ttgggtacaa
      481 tagacgcatg tcatcgatcg accgggcggg gccatcgagc cgctcggcat gaggccgagc
      541 gcccttcaga gcgccatgaa gcgcctgggt atcgccaaga accgtaaaac aaaggaggat
      601 agtatggaaa aaggcaagac tatggacgcg gccctggcta agaaagtctc agggttccag
      661 cgcgacgaga taaccggctg catcctgtac ggcaagcttt ccagggtcat caaggacgcg
      721 aacaacgcca aggtcctggc cgacatggcc caggccgaac ggcagcacta cgagttctgg
      781 aaatcgctgt ccggcaagga cgaggcgccc taccggggac ttgtattttt ctattattgg
      841 tgcgcgcgta tcctcggcct caccttcgcg ctcaagctga tggaaaaggg cgagaaggcc
      901 gcgatcgacg gctaccgggc ggtggcccac ctcgtaccgc aggccgagcg catcggccgg
      961 gaggaggagg agcacgagga ttcgctcttg gccatgctcg acgaggaacg cctggcgtac
     1021 atgggctcca tcgtgctcgg cctcaacgac gcgctggtcg agctgaccgg gacgctggca
     1081 gg
//
LOCUS       DAOPHV010000825         1081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_205438_length_1081_cov_5.022417, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000825 DAOPHV010000000
VERSION     DAOPHV010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205438_length_1081_cov_5.022417"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="PLQ29_14685"
     CDS             complement(<1..1044)
                     /locus_tag="PLQ29_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87940.1"
                     /translation="MPVIALLFVALRLRRRLVYPHELLADRSGARPTALLLRMMRLYF
                     DTALDAICAVVVGLALAGYPRPAADKGPVVVMDASASMLAGMRGDRPLDEAARLALSY
                     YSQDEAGARRTRLFALGWDPTRRRHSLTDRSALLETAKSPQELAAGLESSESFMSVDY
                     ALLAGLARRGYGEVTLLTDDASVEGLGVEIVRLGAKPPRYLLLATAAWDRGSGKSVAR
                     FVAAGGAEPDSLWRLGPGGAAARAKPEDYEIRRSPSGFELALARPGPWAVRWDGRLLP
                     FVAPGPPEPLRARGTMAERVIAGLKPIGGATGRGVMVVDRGGAGRPGSLSVAAARDES
                     WLLLPRTTLGALVA"
BASE COUNT          158 a          397 c          397 g          129 t
ORIGIN      
        1 ggcgacgagg gcgccgaggg tggtcctcgg cagcaggagc cacgactcgt cccgggcggc
       61 ggcgaccgac agcgagcccg gcctgccggc cccgccgcgg tcgacgacca tgacgccccg
      121 cccggtagcg ccgcctatcg gcttgagacc cgcgatgacg cgctcggcca tcgttccgcg
      181 agcccgcagc ggctcgggcg gcccaggcgc gacgaagggc aggagccggc cgtcccatcg
      241 taccgcccag gggccgggcc tcgccaaggc cagctcgaag cccgaggggg agcgtcgtat
      301 ctcgtagtcc tcgggcttgg cgcgcgccgc ggctccgccc ggcccgagcc gccataggct
      361 atcgggctcg gcgccgcccg cggctacaaa gcgcgcgacg ctcttgcccg agccgcggtc
      421 ccaggccgcc gtcgccagga gcaggtagcg cggcggcttg gcccctagcc tgacgatctc
      481 gacgccgagc ccctcgaccg aggcgtcgtc ggtaaggagc gtcacctcgc cgtagcctcg
      541 gcgagccagg cccgcgagca gggcgtagtc gaccgacatg aacgactccg acgattcgag
      601 gcccgccgcc agctcctggg ggctcttggc cgtctcgagc agggcgctcc tatcggtcag
      661 ggagtgcctg cgcctcgtcg ggtcccaacc gagggcgaag agcctcgtcc ggcgcgcgcc
      721 agcctcgtcc tggctgtagt acgacagggc caggcgcgcc gcctcgtcga gagggcggtc
      781 gccgcgcatg cctgccagca tcgaggccga tgcgtccatg acgacaaccg ggcccttgtc
      841 cgccgctggc cgcgggtagc ccgcgagggc cagcccgacg acgacggcgc agatggcgtc
      901 cagggcggtg tcgaagtaga gccgcatcat tctcaggagc agggcggtcg gcctcgcgcc
      961 gctccgatcg gcgaggagct cgtgaggata gaccagcctg cgcctgagcc tgagcgccac
     1021 gaacaggagc gctatgacag gcacgaggag ccaggcgatt ggtccgagcg ccgaggttag
     1081 c
//
LOCUS       DAOPHV010000826         1073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_207943_length_1073_cov_3.376228, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000826 DAOPHV010000000
VERSION     DAOPHV010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207943_length_1073_cov_3.376228"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1073)
                     /locus_tag="PLQ29_14690"
     CDS             complement(<1..>1073)
                     /locus_tag="PLQ29_14690"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPG87941.1"
                     /translation="ITQQDIDDAEVRLRPVLGVAPRLYVAWLYGLGLLAAAFMLLLYP
                     GLRHPGATYSFTVDPPGSAVFIDGAYVGYAPCELFVPAGEHELRVSRPGFADHEAAIT
                     TKGRAFGTLIVKPRSELSVSLGGADVEAVLAGGIERYASWALAGTPSESYQLPMDLSD
                     AVRAAGDVGDNAVGLLGAAASYARHGPSLRDAARAASIAYGRSATVTPVSSGRLVAAL
                     TDELRDDPAILTVLAALGPEAVRARLETSRTYRDALGSSAPAAVAGASSTFAGEAFVA
                     MGCGVSVVGAGAALSAVHRIEPFWIASSETTVGRFRRFIRERPEWAPEEAASLEAGGL
                     ADAAYLVGFGEAADSDPLRYVSK"
BASE COUNT          179 a          360 c          400 g          134 t
ORIGIN      
        1 gtttcgatac gtagcgtagc ggatcggagt cggcagcctc gccgaaaccc accaggtacg
       61 cggcgtcggc gagacccccg gcctccagcg aggcggcctc ctccggcgcc cattcgggac
      121 gctccctgat aaagcgcctg aaacgcccga cggtcgtttc ggatgacgcg atccagaagg
      181 gctcgatacg atgaacggcc gagagggcgg cgccggcccc gacaaccgag acgccgcagc
      241 ccatggcgac gaaggcctcg cctgcgaagg tcgatgaggc gccggcgacg gccgccgggg
      301 cggacgatcc gagcgcgtcg cggtaggtgc gggacgtctc gagccgcgcc cgcaccgcct
      361 ccgggccgag ggctgcgagt acggtcagga tcgcggggtc gtcgcgcagc tcgtctgtca
      421 gggccgcgac gaggcgaccg gagctgaccg gagtgacggt cgccgatcgg ccataggcga
      481 tggaagcggc gcgggcggcg tcgcgtagcg aagggccgtg cctggcgtac gaggcggccg
      541 cgccgaggag accgacggcg ttatcgccga cgtcgcccgc ggcgcggacc gcgtccgaca
      601 ggtccatcgg tagctggtac gattcgctcg gggtcccggc cagggcccag gacgcgtagc
      661 gttcgatccc gcccgcgagc acggcctcga cgtctgctcc accgagcgag acggacagct
      721 cggagcgcgg cttgactatc agcgtgccga aggcccggcc cttcgtcgta atggccgcct
      781 catgatcggc gaaacccggc cgcgagactc ggagctcgtg ctcgcccgcc gggacgaaca
      841 gctcgcaggg cgcatagccg acgtaggcgc cgtcgatgaa cacggcggaa ccgggaggat
      901 cgacggtgaa cgagtaggta gcccctggat gccggaggcc cgggtacagc aggagcatga
      961 aggccgccgc caggaggccg aggccgtaga gccaggcgac gtatagcctc ggtgccacgc
     1021 cgaggaccgg cctgagccgc acttcggcgt cgtctatatc ctgttgggtt atc
//
LOCUS       DAOPHV010000827         1066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_210166_length_1066_cov_2.697329, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000827 DAOPHV010000000
VERSION     DAOPHV010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210166_length_1066_cov_2.697329"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLQ29_14695"
     CDS             complement(<1..328)
                     /locus_tag="PLQ29_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HPG87942.1"
                     /translation="MSLTTRLFILMLFSVMVPMLGMSFFLKSEISESILVEKRDKLFG
                     LARQLDAALEGSYDDILAEEGALDADRDTKIAVLNARLRGITDFVASGNAGVGVGYYG
                     RDLDAVL"
     gene            445..>1066
                     /locus_tag="PLQ29_14700"
     CDS             445..>1066
                     /locus_tag="PLQ29_14700"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPG87943.1"
                     /translation="MNPIFEERFKVCTWDVDRADRLTLAAAFNYFQDAAGHHASDLGV
                     GTEYMKANGQAWILSRMSARIDHRPGWGARLRARTWPRGTDRLFAVRDYELWDEAGTR
                     IGVGRSAWLIVDAATFRPRRPEELAAGLPSNDGLEALPGGGQAIRQSEGLSPVASRTV
                     AYSDLDYNGHMNNARYVQWIQDALDPDELAAAPSLRLDINYLSEMLP"
BASE COUNT          186 a          362 c          345 g          173 t
ORIGIN      
        1 tgagcaccgc gtccaggtcg cggccgtagt agccgacgcc gacgccggcg ttgcccgagg
       61 ccacgaagtc ggttatgccg cgcagcctgg cgttgagcac ggctatcttg gtatcgcgat
      121 cggcgtcgag cgcgccttcc tcggcgagta tatcgtcgta gcttccttcg agagcggcgt
      181 ccagctgcct ggccagtccg aagagcttat cgcgcttctc gacgaggatg ctctcgctta
      241 tctcgctctt cagaaagaac gacatgccga gcatcggcac catcacggag aacagcatca
      301 ggatgaacag ccgggtcgtc aggctcatgg tgtatacaag ccgttccggc gagggttgtc
      361 aatggacgcg gcgcaccatc gccgggctcg tgacaatcgg actccggttg tcacgagccg
      421 tggcccgcgc tatagtggta cgctatgaac ccgatattcg aagaacgctt caaggtatgc
      481 acctgggacg tcgaccgggc cgaccggcta accctggccg cggccttcaa ctattttcag
      541 gacgcggccg gtcatcacgc ttccgacctc ggcgtcggca ccgagtacat gaaggcgaac
      601 ggtcaggcct ggatactgtc caggatgagc gccaggatcg accataggcc cggttggggg
      661 gcgcggctcc gcgcgcgcac ctggccgcga ggcaccgacc gcctcttcgc cgtacgcgac
      721 tacgagctat gggacgaggc cggcacgcgg atcggcgtcg ggcgcagcgc ctggctcatc
      781 gtcgacgccg cgaccttcag gccgcgtcgg cccgaggagt tggcggccgg gctgccgtcc
      841 aacgacggac tcgaggcact ccccggcggc ggccaggcga tacgccagtc cgaaggcctg
      901 tcgcccgtgg ccagccgcac ggtagcctac tcggacctcg actacaacgg ccacatgaac
      961 aacgcccgct acgtgcagtg gatccaggac gcgctcgacc cggacgagct ggcggccgcc
     1021 ccgtcgctcc ggctcgacat caactacctg tcggagatgc tccccg
//
LOCUS       DAOPHV010000828         1062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_211415_length_1062_cov_3.720953, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000828 DAOPHV010000000
VERSION     DAOPHV010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211415_length_1062_cov_3.720953"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1033
                     /gene="secY"
                     /locus_tag="PLQ29_14705"
                     /pseudo
     CDS             1..1033
                     /gene="secY"
                     /locus_tag="PLQ29_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188390.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
BASE COUNT          210 a          332 c          305 g          215 t
ORIGIN      
        1 agcctcaaga agatggcgga ggaggagggc gggcgcaaga agatagcgtt gtacccgcct
       61 cggggccatc gtggtagcga tactccagtc gttcgcgctc gtcgcctacg ccaagaacat
      121 cgcgaagacc catcccgacg tgctcatgat cagcccccag gcctatacgc tgctggccat
      181 gctgacggta accaccggca ccatgttcct catctggata ggcgagcaga tcaccaagcg
      241 cggcatcggt aacggcatat cgctgatcat cttcgcgggc atcgtggcca ggctaccgaa
      301 cgccctgtac gcgctattct ccaagatggt ctcgggcgag gtcaaccccg tcgtcgccat
      361 cttcgtactg ctgctgttcg tcgctgtggt cgtgctggtc atcttcgagc agcagggcca
      421 gaggaagatc ccggtcaact acgccaagcg cgtggtcggt cgcaagatgt acggcgccca
      481 gaacacctac attccgttca agatcaaccc ctccggcgtc atccccgtga tcttcgcctc
      541 cagcctgctg acgttcccgc ttcagatcgc ccagagcctg ggcggcggcg ccaagtggct
      601 gcgcgacttc gcgtacttcc tacggccaga gggttggtgg tacaatatct tctacatcct
      661 gttcatcgtc ttcttcgcgt acttctatac gcaggtgtcg ctgaatccgc aggagataag
      721 caagaacatc cgcgagaacg gcggttcgat tccgggcata cgctcggaga agatggaaga
      781 gtacctgtcc aagatactca accgtatcgt gttgcccggc gccctgtacc tcgcgctcat
      841 agcgctgata ccgacgatcg tgcagcgcgt gttcaatttc ccgagcgagc tttcctacct
      901 gttgggcggt acctcgctcc tcattctagt cggagtcgac ttggatacca tgtcccaggt
      961 ggaggcgcag ctcaagatgc accaccacga cgggctcacg aagcgcggac atatcaagtc
     1021 gcgcaacctg tagcatggta gacgagggcg gcccgggcgt gg
//
LOCUS       DAOPHV010000829         1060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_212140_length_1060_cov_3.102488, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000829 DAOPHV010000000
VERSION     DAOPHV010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212140_length_1060_cov_3.102488"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PLQ29_14710"
     CDS             <1..524
                     /locus_tag="PLQ29_14710"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPG87944.1"
                     /translation="VDRPQVGVEGVAAALGEAVPIAGGALIIIAAARGLSFYTMEAGV
                     PELFAGWITSAVSSRFVFLLLLNLALLVVGCFMDIFSAVLVISPLVIPIGLAYGVHPV
                     HLGVIFIANLSIGFLTPPIGMNLFLASYAFGKPVMRIYKDVLPFFAAQLVVLALITWI
                     PWFSLALLPGGSP"
     gene            521..>1060
                     /locus_tag="PLQ29_14715"
     CDS             521..>1060
                     /locus_tag="PLQ29_14715"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPG87945.1"
                     /translation="MNSTDLPGHGPSTIPIFMRRPGASDAVLLVHGYTGFPGEMGYVA
                     EALFRAGHTVYAPRLPGHGTDRADFMATGADDWIRRARDAYIELAAEYGTVRIVGHSM
                     GGAIAVILAADYAPDRVALLTPAVDIADRRLALAASLGRLVPVIKRDDPPSPEDAEGE
                     RRRLHEEYRKDTLVRQAGEL"
BASE COUNT          149 a          377 c          335 g          199 t
ORIGIN      
        1 ctgtggatcg cccgcaggtc ggcgtcgagg gtgtcgctgc ggccctcggc gaggccgtcc
       61 ccatagccgg aggggccctg atcatcatcg ccgccgccag gggcctgtcc ttctacacga
      121 tggaagccgg agttccggaa ctgttcgccg ggtggatcac ctcggccgtg tcgtcgcgct
      181 tcgtgttcct gttgctcctc aacctggccc tgctcgtcgt cggttgcttc atggacatct
      241 tctccgcggt gctcgtcata tcgcctctcg tcattccgat cgggctggcc tatggcgtcc
      301 acccggtcca cctcggcgtc atcttcatcg ccaacctgtc cataggcttc ctgacgccgc
      361 cgatagggat gaacctgttc ctggcgagct acgccttcgg gaagcccgtc atgaggatct
      421 ataaggacgt actaccgttc ttcgcggccc agctcgtcgt cctcgcgctg atcacctgga
      481 tcccatggtt ctcgttggcg ctactaccag gagggtctcc atgaactcga ccgatctgcc
      541 gggccacggc ccgtcgacca tccccatctt catgcgccgc cctggcgcct cggacgccgt
      601 gctactcgtg cacggctata ccggcttccc gggcgagatg ggctatgtgg ctgaagcgct
      661 gttccgggcc ggccataccg tctacgcgcc tcgcctgccc gggcacggca ccgacagggc
      721 agacttcatg gcgaccggcg ccgatgactg gattaggcgg gcgcgcgacg cgtacatcga
      781 gctggcggcc gagtacggca cggtaaggat agtcggccac tcgatgggcg gcgccatcgc
      841 cgtcatcctg gccgccgact acgcgcccga ccgtgtcgcg ctcctgacgc cggccgtcga
      901 catagccgac cgccgcctcg cgctcgcggc gtcgctcggc cgactcgtgc cggtgatcaa
      961 gcgtgacgac ccgccgtcgc cggaagacgc cgagggagag cgtcgcaggc tccacgagga
     1021 gtaccgtaag gacaccctgg tcaggcaggc cggcgagctg
//
LOCUS       DAOPHV010000830         1060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_212145_length_1060_cov_3.028856, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000830 DAOPHV010000000
VERSION     DAOPHV010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212145_length_1060_cov_3.028856"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLQ29_14720"
     CDS             <1..244
                     /locus_tag="PLQ29_14720"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPG87946.1"
                     /translation="KVETPVPEGIEGRVPYKGELRDYLRQLVAGLKKGMGYTGCGDIE
                     TLRTYRKFVRITGAGLRESHAHDVAITQEAPNYSRR"
     gene            266..>1060
                     /locus_tag="PLQ29_14725"
     CDS             266..>1060
                     /locus_tag="PLQ29_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HPG87947.1"
                     /translation="MNVAVIGAQWGDEGKGKIVDYLATDAQIIVRFSGGANAGHTIVR
                     GGEQFALHLVPSGVLYPDKRVILGTGMVIDPEALFAELGMLAERGIDWEGRVFISDRA
                     HLVLPRYRTLDKSLEAACKRPIGTTGRGIGVAYAMKSSRDGVRLADLFDDRLLENLED
                     DDRRFIAEWRDRLAPLAVDLTHYLSGHRNTYCLFEGAQGALLDLDTGTYPYVSSGMSG
                     AGGAATQGGIGPRRLDKVLGVFKAYSTRVGNGPFPTEFDDEFARTIR"
BASE COUNT          194 a          356 c          359 g          151 t
ORIGIN      
        1 caaggtcgag acgcccgtgc ccgagggcat cgagggccgg gtgccctaca agggcgagct
       61 ccgagactac ctgcgccagc tcgtcgccgg cctcaagaag ggcatgggct ataccggctg
      121 cggggatata gagacgctcc gcacgtacag gaagttcgta cggatcaccg gggccggcct
      181 gcgcgagagc cacgcccacg acgtggccat aacccaggaa gcgccgaact actcgcggcg
      241 ataggcgaag gataaggaga acggcatgaa cgtagcggtt attggcgccc agtggggcga
      301 cgagggcaag ggtaagatag tcgactacct ggcgaccgac gcgcagatca tcgtgcgttt
      361 ctcgggcggc gccaacgctg gccataccat cgtcaggggc ggcgagcagt tcgcgctaca
      421 cctcgtcccc tcgggcgtgc tctacccgga caagcgggtg atactcggca ccggcatggt
      481 catcgacccc gaggccctgt tcgccgagct cggcatgctc gccgagcgcg gcatcgactg
      541 ggaaggccgc gtcttcatct cggaccgggc gcacctcgtg ctaccgcgct accggacgct
      601 cgacaagtcc ctcgaggccg cctgcaagcg gccaatcggc acgaccggcc gcggcatagg
      661 cgtcgcgtac gcaatgaagt cgtcgcgcga cggggtcagg ctggccgacc tcttcgacga
      721 taggctactc gagaacctcg aggacgacga taggcgcttc atagccgagt ggcgcgatag
      781 gctggcgccg ctggccgtcg acctgacgca ctacctctcc ggccaccgga acacgtactg
      841 cctgttcgaa ggcgcgcaag gggccctcct cgacctcgac acggggacct atccctacgt
      901 gtcgtcgggc atgtcggggg cgggaggcgc ggccacccag ggcggcatcg gccccaggcg
      961 cctcgacaag gtgctcgggg tgttcaaggc ctactcgacc cgagtcggga acggcccctt
     1021 cccgaccgaa ttcgacgacg agttcgcgcg cacgatccgc
//
LOCUS       DAOPHV010000831         1059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_212476_length_1059_cov_3.277888, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000831 DAOPHV010000000
VERSION     DAOPHV010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212476_length_1059_cov_3.277888"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /gene="pepT"
                     /locus_tag="PLQ29_14730"
     CDS             complement(<1..874)
                     /gene="pepT"
                     /locus_tag="PLQ29_14730"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF003976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripeptide aminopeptidase PepT"
                     /protein_id="HPG87948.1"
                     /translation="MKDFADILARPHTAETIERLVRYAKIETTSDRHVDVIPSTPSQW
                     NLARLLESEIRALGVADVSVDEHCFVIARVPATPGYEKKPTIGLMAHLDTSSEVSGAD
                     VKPRVVEGYDGKALTLSPGWVLDPDEYAELRDYAGDTIVVTDGTTLLGADDKGGIAVV
                     MTAIAAVLKDPSFAHGPIVLVFSPDEETGKGMDGFPAQAVRMDACYTFDGGKGGEIES
                     ECFTAYEAKLAFAGKSSHPGYARGVMANAVSMAASFVAMLPRSESPEATDGWYGYYYA
                     HEIHGGLESASVDVL"
BASE COUNT          162 a          342 c          374 g          181 t
ORIGIN      
        1 gcagcacgtc gacgctggcc gattcgaggc cgccatggat ctcatgggcg tagtagtagc
       61 cgtaccagcc gtcggtcgcc tcggggctct cgcttcgcgg cagcatcgcg acgaagctgg
      121 ccgccatcga cacggcgttg gccatcacgc cgcgggcgta gccgggatgg gacgacttgc
      181 cggcgaaggc cagcttggcc tcgtaggcgg tgaagcactc ggattcgatc tcgcctccct
      241 tgccgccgtc gaaggtatag caggcgtcca tccgcaccgc ctgggcgggg aagccgtcca
      301 tgcccttgcc ggtctcctcg tcgggggaga agacgaggac gatggggccg tgcgcgaagg
      361 aagggtcctt gaggacggcg gcgatcgcgg tcatcacgac ggcgatgccg cccttgtcgt
      421 cggccccgag cagggtggtg ccgtcggtca cgacgatggt gtcgccggcg tagtccctga
      481 gctcggcgta ctcatctggg tccaggaccc agcccggcga cagagtcagg gccttgccgt
      541 cgtagccctc gacgactcgc ggcttaacgt cggcgccgga tacctcgctg gaggtgtcca
      601 ggtgggccat caggcctatc gtcggcttct tctcgtagcc gggcgtggcc gggacgcggg
      661 cgatgacgaa gcagtgctcg tcgacgctga cgtcggcgac gccgagcgcc ctgatctctg
      721 actcgaggag cctggcgagg ttccactggg acggagtcga cgggatgacg tcgacgtgac
      781 gatcgctcgt ggtctctatc ttggcgtagc gcacgagccg ctcgatagtc tcggccgtat
      841 gaggcctggc aagtatgtcg gcgaagtctt tcataccacg agagtatgcc ttcagccacg
      901 gctctatgca agccgggctc gcgtttctcc cgggagcgga cgaggcgcgc accgccacac
      961 gccgccccct ccgacgctag gcctgcaaga gctcggccag gccgagattc ctgaaggcgg
     1021 tcgctacctc ggagagcgcg tcgaggtggg cctccggac
//
LOCUS       DAOPHV010000832         1059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_212601_length_1059_cov_2.312749, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000832 DAOPHV010000000
VERSION     DAOPHV010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212601_length_1059_cov_2.312749"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="PLQ29_14735"
     CDS             complement(<1..171)
                     /locus_tag="PLQ29_14735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG87949.1"
                     /translation="MSDILRVRGLTKRFGGLAAVNEVDFDVREGGISGLIGPNGAGKT
                     TIFNLITGVYKVS"
     gene            complement(168..>1059)
                     /locus_tag="PLQ29_14740"
     CDS             complement(168..>1059)
                     /locus_tag="PLQ29_14740"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPG87950.1"
                     /translation="INLIAVLGLSLLTGFAGLFSFGHAGFMAIGAYSAAICTKLLGLP
                     FFAGIAAAAAVAAGVSWLIGRATLKLKGDYFCIATLGFGEAIRLILDNFQGLGGARGW
                     PGIPPHTTLLNILILDVIGVVLLANLVRSRHGRNMIAVREEELAAKVAGIDTFRYKML
                     ALVISAAYAGVAGALLGHYMTFIQPKMFSMVKSTELTIIVIFGGLGSISGSVLGAVVL
                     TFLPELLRAVDKWRLVVYGLAVIGIMIGRPRGLMGGFELTPKSIRAHFARRREERAIK
                     AGQAAEDEAFAGGEDGKGGL"
BASE COUNT          180 a          353 c          359 g          167 t
ORIGIN      
        1 gctgaccttg tagacgccgg tgatcaggtt gaagatcgtg gtcttgccgg cgccgttcgg
       61 cccgatgagg ccggagatgc ctccctcccg cacgtcgaag tccacctcgt tgacggccgc
      121 gagcccgccg aagcgcttgg tcagtccgcg gacccggagt atgtcgctca tagcccgccc
      181 tttccgtctt cgccgccggc gaacgcctcg tcctcggccg cctggcccgc cttgatggcg
      241 cgctcctccc gacggcgagc gaaatgcgcc ctgatggact tcggcgtgag ctcgaagccg
      301 cccatcaggc cgcgcggcct gcctatcatg atgccgatga ccgcaaggcc gtagacgacc
      361 aggcgccact tgtcgaccgc gcgcagtagc tcgggcagga aggtgagcac cacggccccg
      421 agcacggagc cggagatcga gccgaggccg ccgaagatga cgatgatggt cagctcggtc
      481 gacttgacca tgctgaacat cttgggctgg atgaaggtca tgtagtggcc gagcagggcc
      541 ccggctacgc ccgcgtacgc ggcgctgatg acgagcgcca gcatcttgta gcggaacgtg
      601 tcgatgccgg ccaccttggc cgcgagctcc tcctcgcgta cggctatcat gttgcggccg
      661 tgccgcgagc gcacgaggtt ggccaatagc acaacgccga tcacgtcgag tatcaggatg
      721 ttgagcaggg tggtgtgcgg cgggatgccg ggccagccgc gcgcgccgcc gagcccctgg
      781 aaattgtcga ggatgagccg tatggcctcg ccgaagccga gcgtcgctat gcagaaatag
      841 tcgcccttga gcttgagcgt ggctcggcct atcagccagc tgacgccggc cgctaccgcc
      901 gccgccgccg cgatgcccgc gaagaacggc agcccgagca gcttggtgca gatggccgcc
      961 gagtaggcgc ctatcgccat gaagccggcg tggccgaacg agaacagccc cgcgaagccc
     1021 gtgagcaggc tcaggcctag cacggctatg aggttgatg
//
LOCUS       DAOPHV010000833         1058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_212762_length_1058_cov_4.125623, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000833 DAOPHV010000000
VERSION     DAOPHV010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212762_length_1058_cov_4.125623"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..>1058
                     /gene="pyrF"
                     /locus_tag="PLQ29_14745"
     CDS             245..>1058
                     /gene="pyrF"
                     /locus_tag="PLQ29_14745"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPG87951.1"
                     /translation="MQFFDRIEAAQVERGTALCVGLDPRLGPGEGPDDLFRRSLAIAE
                     ATARYAACFKPNAAFYEAFGAAGLDALVRLVKAMPGGVPVLLDAKRCDIGSTAEAYAK
                     ACFEAIGAGAVTLSPYMGRDAVDPFLAYPDKAVFLLARTSNPRAGVFQDLEAGGDSLY
                     ARVAAECASWSDRVGLVAAGNDPHGLSIVRAAAPCAWLLAPGIGAQGGDIAEAWRAGA
                     RADGRGILPVAARAVAEAPDPSAAAAELVASMRRAAEAGTRVAVSGYDRKSLL"
BASE COUNT          168 a          349 c          357 g          184 t
ORIGIN      
        1 ctaagggcat tgtatgcctc attgtagtga tatcaatgcc cttagccttc ggcgtcttcg
       61 ccgaaggcta agggcattga tatcactaca atgaggcata ctataagaat ttttcgcatc
      121 gcgcgctcct cctgcatcat gagtatctcc atgcgatact cgcaagtata gccctttttg
      181 actataaaat caccacgcgc tatgctcggg gcccgtcgat ggcatcgacg aaaggcggga
      241 aaccatgcag ttcttcgaca ggatcgaggc ggctcaggtc gagcggggca cggccctatg
      301 cgtcgggttg gatccgaggc ttggaccggg ggaggggccg gacgacttgt tccgccgctc
      361 gctggctata gccgaggcca cggccagata cgcggcctgt ttcaagccga acgccgcgtt
      421 ctacgaagcc ttcggagccg cggggttgga cgcgctggtc cgcctcgtca aggcgatgcc
      481 gggcggggtg ccggtcctgc tcgacgccaa gcgctgcgac atcggctcga ccgccgaagc
      541 ctatgccaag gcctgcttcg aggcgatcgg cgccggcgcc gtgacgctca gcccctacat
      601 ggggcgcgac gcggtcgacc ccttcctggc ctacccggac aaggcggtat tcctgcttgc
      661 caggacgagc aatccgcggg ccggcgtctt ccaagacctc gaggcgggcg gcgatagcct
      721 ctacgcccgt gtggctgccg aatgcgcttc gtggtccgac cgggtagggc tcgtcgccgc
      781 cgggaacgac ccgcacggcc tgtccattgt ccgtgcggcc gcgccttgcg cctggctact
      841 ggcgccgggc ataggcgccc agggcggcga catcgccgag gcctggagag ccggcgcgcg
      901 ggccgacggg agaggaatcc tgccggtagc cgcgagggcc gtcgccgagg ctcccgatcc
      961 gtcggccgcg gcggccgagc tggtcgcctc catgcgccgg gccgccgagg ccggcacgcg
     1021 cgtcgcggtc tccggctacg accgtaagag cctcttga
//
LOCUS       DAOPHV010000834         1058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_212764_length_1058_cov_4.003988, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000834 DAOPHV010000000
VERSION     DAOPHV010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212764_length_1058_cov_4.003988"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PLQ29_14750"
     CDS             complement(<1..1007)
                     /locus_tag="PLQ29_14750"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87952.1"
                     /translation="MEESLDAVGLGVGDLAGVFHTHVHDDHFVGLTSLMRADKRLSYY
                     AVPMVRRSATEKLRALCGLGADEFARLFDVVDLRPDEWSDVGGFDVKPVYSPHPLETT
                     ILRFRVAVADGYRTYAHYADLSSFAVIDSMVTDDASVAGITAAEASRAKAAYLEPADV
                     KKVDVGGGMIHGDPDDFAGDRSGDLLLSHGDSAEALSGKGFGRIASFGEVSLLAPERR
                     DWAAEAAADALRGYFPDAPAEAIEALASGPRRERKAGDRLLAAGNEPPAALLTLDGRV
                     TRVDGVGPAASYGPGALLGAAAVLRGEAEPDDWVVERDTAIVELPAEAFAAFARKNGL
                     EA"
BASE COUNT          156 a          381 c          370 g          151 t
ORIGIN      
        1 gcctcgaggc cgttcttcct cgcgaacgcc gcgaacgcct ccgccggtag ttcgacgatg
       61 gccgtgtcgc gctcgacgac ccagtcgtct ggctcggctt cgccgcgcag cacggcggcc
      121 gccccgagca aggcgcccgg cccgtatgac gccgctggcc cgacgccgtc gacacgcgtc
      181 acccggccgt caagggtcag cagcgcggcc ggaggctcgt ttccggcggc gagcaggcga
      241 tcgcccgcct tgcgctcgcg ccttgggccc gaggccaggg cctcgatcgc ctcggcgggg
      301 gcgtcgggaa agtagccccg gagcgcgtcc gcggccgcct cggcggccca gtcccgccgc
      361 tcgggcgcca gtaggctgac ctcgccgaac gaggcaatcc tcccgaagcc cttgcctgac
      421 agcgcttccg ccgagtcacc gtggctcagg agcaggtcgc ccgagcgatc gccggcgaaa
      481 tcatccgggt cgccgtgtat catgccgccg ccgacgtcca ccttcttgac gtccgccggc
      541 tcgaggtagg ccgccttggc gcgcgacgcc tcggcggccg tgatcccggc gaccgaggcg
      601 tcgtccgtga ccatcgagtc gatcaccgcg aacgagctca ggtcggcgta atgggcgtag
      661 gtcctatacc cgtccgcgac ggcgaccctg aaccggagga tagtcgtctc gagcgggtgc
      721 ggcgagtaga ccggcttgac atcgaagccg ccgacgtcgc tccactcgtc tggccgcagg
      781 tcgaccacgt cgaagaggcg ggcgaactcg tcggcgccga ggccgcagag ggcccgaagc
      841 ttctcggtag ccgagcgtct gaccatcggc acggcgtagt acgacagccg tttgtcggcc
      901 cgcatcaggc tggtcaagcc gacgaagtgg tcatcgtgga cgtgcgtatg gaagacgcca
      961 gcgaggtcgc cgacgccgag gccgaccgcg tcgaggctct cctcgatgtt cgggccagca
     1021 tcgataaggt agcggcgccc agcgtggacg acgaggct
//
LOCUS       DAOPHV010000835         1054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_214086_length_1054_cov_3.399399, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000835 DAOPHV010000000
VERSION     DAOPHV010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_214086_length_1054_cov_3.399399"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PLQ29_14755"
     CDS             <1..688
                     /locus_tag="PLQ29_14755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPG87953.1"
                     /translation="PSAEEEQASLDTDSCEKAHEGSLWSALRSRPTLLVFAALTTFYG
                     FSYAQHRFAMPLQATSLFGDSGARLYGFMMTLNAVCVVALTTPLVALTRRVRPVFNVA
                     LAGALYAVGFGMLALARSPWLFFASTVLWTVGEIVQATNESVYVSNHTPMSHRGRFNA
                     VLPLISGLGFSLSTPIAGRIADRSGLSAVWIVVAASTGLAAVGVWTLGRYEKRTAPAG
                     RAGTGDEASV"
     gene            complement(733..>1054)
                     /locus_tag="PLQ29_14760"
     CDS             complement(733..>1054)
                     /locus_tag="PLQ29_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87954.1"
                     /translation="GALLHDFFLYDWRDGTNPRHPTGHARVALANARARFELNPVEED
                     IIVSHMWPIGKPFYSYSESALVSLVDKYVSTMEVALLFVRTLRSFGYAVAQRLKARLI
                     ASRA"
BASE COUNT          158 a          340 c          369 g          187 t
ORIGIN      
        1 gccgagtgcc gaggaagagc aggcgagcct ggatactgac tcgtgcgaga aagcccacga
       61 gggtagcctc tggtcggcgc tacgctcgag gcctaccctc ctcgtcttcg ccgcgctgac
      121 gaccttctac ggcttttcgt acgcgcagca ccgcttcgcg atgccgctcc aggcgacgag
      181 cctgttcggc gactcgggcg ccaggctgta cggcttcatg atgacgttga acgccgtctg
      241 cgtggtcgcg ctgacgacgc cgctcgtcgc cctgacgcgg cgagtacgcc cggtcttcaa
      301 cgtggcgcta gccggcgccc tgtacgccgt gggcttcggc atgctcgcct tagcccgctc
      361 gccgtggctg ttcttcgcgt cgaccgtact atggacggtc ggcgagatcg tgcaggcgac
      421 gaacgagagc gtctatgtgt cgaaccatac gccgatgtcg catcgcggcc gcttcaacgc
      481 ggtgctaccg ctgatatccg gcctgggctt ctcgctgtcc acgccgatag cgggccgtat
      541 cgcggaccgc tccgggctat cggcggtatg gatcgtcgtg gcggcgtcca cgggcctggc
      601 cgccgtcggc gtctggacgc tcgggcgcta cgagaagcgt acagctccgg ccggccgagc
      661 cggaacgggt gacgaggcga gcgtctgagg atgcccggtt ccgaggctcg gcgtacgcgc
      721 cgcggcgagc ctctacgctc ttgaggctat caagcgtgcc ttgaggcgct gcgctacggc
      781 gtagccgaag gatcgcaggg ttcggacgaa caacagggcg acctccatcg tcgacacgta
      841 cttgtcgacg aggctgacga gggccgactc gctgtacgag tagaatggct tgcctatggg
      901 ccacatgtgc gagacgatga tgtcctcctc gaccgggttg agctcgaagc gggccctggc
      961 gttggccaac gccacgcgcg cgtggccggt cgggtgccgg ggattcgtac cgtcgcgcca
     1021 gtcgtacagg aagaagtcgt gcaggagggc gccg
//
LOCUS       DAOPHV010000836         1052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_214785_length_1052_cov_3.084253, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000836 DAOPHV010000000
VERSION     DAOPHV010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_214785_length_1052_cov_3.084253"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1052)
                     /locus_tag="PLQ29_14765"
     CDS             complement(<1..>1052)
                     /locus_tag="PLQ29_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010258822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HPG87955.1"
                     /translation="QRPGGYQGQRPGGYQGGGSSGGYQGQRPGGYQGGGSSGGYQGQR
                     PGAPSGGYGSRPGGYQGSRPGYGPRPGGPGGPGRPGGPGRPGGPGRPGSGGPGSSPLG
                     ADKKPTSKRFIKAKKPSYQRKEESHEQKLHAQRKKADAPKANPIPKSIDIMETVSVSE
                     LAKKMNLKPAELIGKLMSLGFMATINQQIDSDTATILANEYGCEVNIVSLYDETIIEK
                     ETDKPEDLAHRPPIVTIMGHVDHGKTKLLDAIRKTNVIASEFGGITQHIGAYQVETPK
                     GRITFLDTPGHEAFTKMRARGSQVTDIVILVVAANDGVMPQTLEAIDHAKAAKVPIIV
                     AINKIDLPEANPDRVKT"
BASE COUNT          139 a          332 c          347 g          234 t
ORIGIN      
        1 gtcttgacgc ggtccgggtt cgcctcgggg aggtcaatct tgttgatggc gacgatgatc
       61 ggcaccttgg ccgccttggc atggtcgatg gcctcgaggg tctggggcat cacgccgtcg
      121 ttcgcggcga cgacgaggat gacgatatcc gttacctgcg aacccctggc gcgcatcttg
      181 gtgaacgcct cgtgtccggg ggtgtcgagg aaggtaatcc tgccttttgg cgtctcgacc
      241 tgataggctc cgatatgctg cgtgatgccg ccgaactcgc tggctatcac gttagtcttg
      301 cgtatcgcgt caaggagctt ggtcttaccg tggtcgacgt ggcccatgat cgtgacgatg
      361 ggcggcctat gggccagatc ctcaggcttg tcggtttctt tctcgatgat ggtctcgtcg
      421 tacagcgata cgatattaac ctcgcaaccg tactcgttgg ccaggatggt ggccgtgtcg
      481 gagtcgatct gctggttgat ggtcgccatg aacccgaggc tcatcagctt acctatgagc
      541 tcggccggct tgaggttcat cttcttggcg agctccgata ccgagaccgt ctccataata
      601 tcgatggact tcgggatcgg attcgccttc ggggcgtctg ccttcttacg ctgggcatgg
      661 agcttctgct cgtggctctc ttccttgcgc tggtaggaag gcttcttggc ctttataaag
      721 cgcttggagg tgggcttctt gtccgctccg aggggagagg aaccggggcc gccggatccc
      781 ggcctaccgg gtccgcccgg cctaccgggt ccgcccggcc tgccggggcc gcccggtccg
      841 cccggcctcg ggccgtagcc cggcctggat ccctggtatc cacccggcct cgaaccgtag
      901 ccgccggacg gggcaccggg tctctggcct tggtagccgc cggacgaacc gccgccctgg
      961 tagccgccgg gcctctggcc ctggtagccg ccggacgaac cgccgccctg gtagccgccg
     1021 ggcctctgac cctggtagcc gccgggcctc tg
//
LOCUS       DAOPHV010000837         1051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_215067_length_1051_cov_5.108434, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000837 DAOPHV010000000
VERSION     DAOPHV010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215067_length_1051_cov_5.108434"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..484)
                     /locus_tag="PLQ29_14770"
     CDS             complement(218..484)
                     /locus_tag="PLQ29_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPG87956.1"
                     /translation="MKVNPLGDRVLIKVQEGDTKTASGIIIPQTAQEKTQTGVVVAIG
                     TDTDVIKVKKNDKVMYDKYAGTQIKIDNAEHLIVKFSDILAILE"
     gene            628..>1051
                     /locus_tag="PLQ29_14775"
     CDS             628..>1051
                     /locus_tag="PLQ29_14775"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HPG87957.1"
                     /translation="MPRKNDSWNSKKPKKPLKGAPIPPINGWKYTIAYVCIAVISITL
                     FNWFVESADSSIVPYSEFKSLVASGQIERIEMDSNYYTGYGPTSARQDGRPSGQSDGR
                     VFKTVPIYDVEFTKLLDEKGVTYSAVSREGNAMLGFVLN"
BASE COUNT          211 a          308 c          319 g          213 t
ORIGIN      
        1 agaagacggg ccctcacctg cagttcctcg ccgaggtgag ccagctgttc aagagcgaga
       61 cgaagcgggc cgccgcgatg gccgcccgta ctcccgccga gctgctcgcg gtcatcaccc
      121 actgagcctg tagcgggcgc ccagcgatcc catagacacg acggccgccg gatcgatcac
      181 gatccggcgg ccgtttcgat cctcggacgg aaccgtacta ctcgaggatg gcgaggatgt
      241 cggagaactt gacgatcagg tgctcggcgt tgtcgatctt gatctgggtg ccggcgtact
      301 tgtcgtacat taccttgtca ttcttcttga ccttgatgac gtcggtgtcc gtgccgatgg
      361 ctacgacgac gccggtctgg gtcttctcct gggcggtctg cgggatgatg atgccgctgg
      421 cggtcttggt atcgccttcc tgaaccttga tgagcacgcg atcgcccaac gggtttactt
      481 tcatgtcgta tcctcctgga acctgtgtga gtggtttcgt tgacggacga aatgtactaa
      541 aaacgacatg gaggggtcaa ggaagaaagg cgggcgcggc tggccgatac gacgcctgaa
      601 ttcgtacatt atacgtcgga ggaaacgatg ccccgcaaga acgactcatg gaactccaag
      661 aagcctaaga aaccgttgaa gggggcgcct atccccccga taaacggctg gaaatatacc
      721 atcgcctacg tatgcatcgc cgtcatctcg ataaccctgt tcaattggtt cgtcgagtcg
      781 gccgacagct ccatcgtgcc gtacagcgaa ttcaagtcgc tcgtcgcatc gggccagatc
      841 gagcgcatcg agatggactc gaactactac accggctacg ggccgacgag cgccaggcag
      901 gacggtcggc cctcggggca gtccgacggc cgcgtcttca agaccgtgcc gatctacgat
      961 gtggaattca ccaagttgct cgacgagaag ggcgtgacct attccgcggt gtcgcgggag
     1021 ggcaacgcca tgctcggctt cgtgctcaat t
//
LOCUS       DAOPHV010000838         1050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_215411_length_1050_cov_4.569849, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000838 DAOPHV010000000
VERSION     DAOPHV010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215411_length_1050_cov_4.569849"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLQ29_14780"
     CDS             complement(<1..292)
                     /locus_tag="PLQ29_14780"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPG87958.1"
                     /translation="MGKYDEYREKVVEYSKMVFSKGYTAGSGGNVSMLIEGENALAIT
                     PSGKDYSRFTPAEICIVDLDLQPIEGTMRPSIETGMHAAVYKNRRDVNAIIHA"
     gene            complement(303..>1050)
                     /locus_tag="PLQ29_14785"
     CDS             complement(303..>1050)
                     /locus_tag="PLQ29_14785"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPG87959.1"
                     /translation="FERNEKEGGTAAVILEPLGPESGTRPVVYDYNKRVRELCDGFGS
                     LLIFDEVVTAFRVGLGGAQGFFGVKPDLTVFGKCVAGGYPAAGGVGGRRDVMECFAAG
                     IQGMKKRAMVGGTLSANPLSCAAGYYAIKAMEDTNAPVVAGRAGDRLTRGLQEIIERR
                     GLPFVAFNHGSICHLETAAAMLMDINNPKIFTEVTERKHIMEEMGAAMMAEGIISLAG
                     SRLYTSMADTDEVIDDALERFDRVLSCVEI"
BASE COUNT          198 a          323 c          277 g          252 t
ORIGIN      
        1 gcgcgtggat tatcgcgttt acgtcgcggc ggtttttata caccgcggcg tgcatgccgg
       61 tttcgatcga gggacgcatg gtcccctcga taggttgaag atcaaggtct actatacaga
      121 tttcggcagg tgtaaatctc gaataatcct tacccgacgg cgtgattgcg agagcgttct
      181 ctccttcgat gagcattgag acattgccgc ccgagccggc ggtatacccc ttcgaaaaga
      241 ccattttact gtattcgacg actttttcgc ggtattcgtc gtattttccc atttccgcct
      301 ccttatattt ctacgcagga gagtacacgg tcgaagcgtt cgagcgcgtc gtctataacc
      361 tcgtcagtat cggccatgga tgtatacagc ctggagccgg cgagcgatat tatgccttcg
      421 gccatcatag cggcccccat ttcctccatg atatgctttc gctcggtaac ctcggtgaat
      481 atcttcggat tatttatatc cattagcatc gcggcggccg tctccagatg gcagatggag
      541 ccgtggttga aggccacgaa ggggagccca cgcctctcta tgatctcctg aagccctctg
      601 gtcaggcggt cgccggcccg gcccgcgact accggcgcgt tcgtatcctc catcgcttta
      661 atcgcgtaat agccggccgc gcaggaaagc ggattggctg agagggtgcc tcccaccatc
      721 gcgcgctttt tcatgccctg tatgccggct gcaaagcatt ccattacatc ccgtctgccg
      781 ccgactcccc ccgccgccgg gtaaccgccc gctacgcatt ttccgaacac tgttaagtcg
      841 ggcttcacac cgaagaagcc ctgggcgccg cctaagccca ctctaaaggc cgttacgact
      901 tcgtcaaaga tgagtagaga gccgaagccg tcgcaaagct cgcgcacgcg cttattgtaa
      961 tcgtaaacca cgggccttgt gccgctctcc gggcctagcg gttcgaggat gaccgccgcg
     1021 gtgcctccct ccttttcgtt gcgctcgaac
//
LOCUS       DAOPHV010000839         1046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_216753_length_1046_cov_3.671039, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000839 DAOPHV010000000
VERSION     DAOPHV010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216753_length_1046_cov_3.671039"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PLQ29_14790"
     CDS             complement(<1..525)
                     /locus_tag="PLQ29_14790"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPG87960.1"
                     /translation="MKMIDLTIPLGIGTPPWPTYEPLEVKYFKRLAPNGANGQLVTHS
                     NHIGTHLDGEIHFYTPGKDIASLDMDFLVHEGAVVDLSDIAGDYDVYTSKMVEDRVEV
                     KEGDILIIHTGFHHYGWDQPTGDEIRYMIKHPGPDREFAEWAKKKKLRWIGVDCGSAD
                     HPMNTKIRDWMPKQA"
     gene            complement(687..1004)
                     /locus_tag="PLQ29_14795"
     CDS             complement(687..1004)
                     /locus_tag="PLQ29_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87961.1"
                     /translation="MAAAPAIVKFVGGSPRDAIDVTMRMYEITNAESEAYRIPALDFR
                     GTPTGIDVRKVVETGILPAINTGIAHKDPGVGMVGAGLVKPPENAFRDALIAFAEKYA
                     KKL"
BASE COUNT          174 a          317 c          327 g          228 t
ORIGIN      
        1 ggcctgcttg ggcatccagt cgcggatctt cgtgttcatc gggtgatcgg ccgagccgca
       61 gtccacgcca atccagcgca gcttcttctt cttggcccat tcggcgaact cgcggtccgg
      121 ccccgggtgc ttgatcatgt agcggatctc gtcgccggtc ggctggtccc agccgtagtg
      181 gtggaagccg gtatggatta tcaggatatc gccttccttg acctcgacgc gatcctcgac
      241 catcttggag gtgtacacgt cgtagtcgcc ggcgatgtcg gacaggtcga cgacggcgcc
      301 ctcgtgcacg aggaaatcca tgtccaggct cgctatatcc ttgcccggcg tgtagaagtg
      361 gatctcgccg tcgaggtgcg tgccgatgtg gttcgagtgg gtgacgagct gcccgttggc
      421 gccgttcggc gcgagacgct tgaagtactt gacctcgagc ggctcgtagg tgggccaggg
      481 cggggtgccg atgccgagcg gaatcgtcaa atcgatcatc ttcataagaa cctcctgtag
      541 attaactgaa cgaatcagac cacggaggca cggagccacg gagcgccgga atgactcgag
      601 ataaatcaag tgatctaata ttcacaagta tcccgcttca ttttttccga ctccgtgcct
      661 ccgtgcctcc gtggttctct tgtcgattat agcttcttcg cgtacttctc ggcgaaggcg
      721 ataagggcgt ccctgaaggc gttctcgggc ggcttgacga ggccggcgcc gaccatgccg
      781 acgccggggt ccttgtgagc tatgccggtg ttgatcgccg gcagtatgcc cgtctcgacc
      841 accttgcgga cgtcgatgcc cgtcggggtg cccctgaagt cgagcgccgg tatgcggtag
      901 gcctcgctct cagcgttcgt gatctcgtac atcctcatag tcacgtcgat cgcgtccctg
      961 ggcgaaccgc cgacgaactt gacgatagcc ggggcggccg ccatcgcgga ctacatccgc
     1021 cagcgcggcg actccggtca tcaggt
//
LOCUS       DAOPHV010000840         1044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_217486_length_1044_cov_2.694641, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000840 DAOPHV010000000
VERSION     DAOPHV010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_217486_length_1044_cov_2.694641"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PLQ29_14800"
     CDS             <1..783
                     /locus_tag="PLQ29_14800"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HPG87962.1"
                     /translation="DFELPVFAAPVGAVGLHYSDAYTDLSYSAAVLEGCKAAGSAAFT
                     GDGVKDEVYRGTIEAIKAMGGHGVPTIKPWSVAEVIAKAKMAEDAGAFAFAMDIDAAG
                     LVVLAMQGKPVAPMPVASLSKIASSVRMPFILKGVMTPAGALKALEAGAQAIVVSNHG
                     GRVLDETPSTIEALPPIVEALHGRMKVLIDGGFRTGLDVFKALALGADGVLIARPFTW
                     SVYGGGAEGAKLYLEKVKAELAEAMLMTGASSLEEIDGSRLA"
     gene            complement(821..>1044)
                     /locus_tag="PLQ29_14805"
     CDS             complement(821..>1044)
                     /locus_tag="PLQ29_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87963.1"
                     /translation="AFRPYLVEFKGGTAVTFYKDGKVRSGTLSTRQDLIQSIEPNGGD
                     PRYRSVDAGQEVEFDHNGFILAPGDDRTL"
BASE COUNT          184 a          312 c          363 g          185 t
ORIGIN      
        1 gacttcgagc taccggtctt cgcagcgccg gtcggggcgg tcggcctgca ctacagcgac
       61 gcctataccg acctgagcta ctccgcggcc gtgctcgagg gctgtaaagc cgccggcagc
      121 gcggccttca ccggagacgg cgtcaaggac gaggtttacc gcggtaccat cgaggcgatc
      181 aaggcgatgg gcggccatgg ggtgccgacc atcaaaccgt ggagcgtggc ggaggtcata
      241 gccaaggcga agatggccga ggacgccggc gcgttcgcct tcgcgatgga catcgacgcc
      301 gccggtctcg tggtgcttgc gatgcagggt aagcccgtcg cgccgatgcc ggtcgcgtcg
      361 ctgtccaaga tcgcgtcgtc ggtacggatg ccgttcatcc taaagggcgt gatgacccct
      421 gctggggcgc tcaaggccct cgaggcaggc gcccaggcca tcgtcgtctc gaaccacggc
      481 gggcgcgtgc tcgacgagac gccatcgacg atagaggcct tgccgcccat cgtcgaggcg
      541 cttcacggac gcatgaaagt actgatagac ggcggcttca ggaccggcct cgacgtattc
      601 aaggcgctgg ccctgggcgc ggacggtgtg ctgatagcca ggccctttac ctggtcggta
      661 tacggcgggg gcgccgaggg cgctaagcta tatctggaga aggtcaaggc cgaactggcg
      721 gaggctatgc tgatgaccgg ggcctcaagc ctcgaggaaa ttgacgggag caggctggct
      781 taagcgtcgg gtgccgggat cttcggatcc cggcgttccc tcagagagtt cggtcgtccc
      841 cgggggcgag tatgaaaccg ttatggtcga attcgacctc ttgccccgca tccacggatc
      901 ggtatcgagg gtctccaccg ttcggctcga tggactggat caggtcttgc cttgtagaca
      961 gggtgcctga acggaccttg ccgtccttgt agaaggtaac ggcggtaccg cccttgaatt
     1021 cgaccagata gggcctgaag gcca
//
LOCUS       DAOPHV010000841         1041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_218421_length_1041_cov_2.973631, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000841 DAOPHV010000000
VERSION     DAOPHV010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_218421_length_1041_cov_2.973631"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1041
                     /locus_tag="PLQ29_14810"
     CDS             <1..>1041
                     /locus_tag="PLQ29_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87964.1"
                     /translation="ELAETLRSARSDRLYIRTSPDERVAVTVVGRSAAGQGLSDEARA
                     ALDAVLGIPRPEAEAGSDGFLARLGAGAALEAYPRLYDSGDPGTPWSWAERLPLLSIP
                     IELGARRAEGAIDPGAPAPTGALYARIIVDLREDHNALDGTVTPANVSNWIESADYID
                     YTFPHEAFIAGEYGALSASFGRDRLRWGPGSALTLSEEPDYYDYLDAALTTRRFSYRY
                     AWISIDPSLRPSELDALDGGAFESVKNLFLHRLEFLPFDRVAVAIVEGLMLGGVEPDL
                     AYANPFLILHNRFPWNTTAYDGIGGNDFVPAATVVALELRVNPWRYAEVYGTLAMNQL
                     QSSYEIERYGAAA"
BASE COUNT          152 a          358 c          365 g          166 t
ORIGIN      
        1 cgagctcgcc gagacactgc gctccgcacg ctcggaccgc ctctacatcc ggacatcacc
       61 cgacgagcgg gtcgcggtga ccgtggtggg ccgctcggcg gccggccagg gcctgtccga
      121 cgaggcccgg gcggcgctgg acgccgtcct gggtatcccg cggccggagg ccgaggccgg
      181 ttccgatggc ttcctcgcca ggctaggggc gggggcggcg ctcgaggcct atccccgcct
      241 gtacgatagc ggtgatccgg ggacgccctg gtcgtgggcg gagcgcttac cgttactctc
      301 cataccgatc gagctcgggg cgcgtcgcgc cgaaggcgcg atcgacccgg gcgcgcccgc
      361 cccgacgggc gcgctgtacg cacggatcat cgtcgacctc agggaagacc acaatgcgct
      421 cgacggaacg gtgacgccgg ccaatgtctc gaattggatc gagagcgccg actacatcga
      481 ttataccttc ccgcacgagg cgttcatcgc gggcgagtac ggggcgctgt ccgcttcctt
      541 cgggcgcgac cggctgcgct ggggacccgg ctcggccctg acgctgtcgg aggagcccga
      601 ctactacgac tacctggacg cggcgctgac gacgaggcgc ttctcgtacc gctacgcctg
      661 gatatcgatc gacccgtcgc tgcgcccgtc cgagctcgac gccctggacg gcggcgcgtt
      721 cgagtcggtc aagaacctct tcctgcaccg gctggagttc ctgcccttcg accgggtggc
      781 cgtggccatc gtcgaaggcc tgatgctcgg cggcgtggag ccggatctgg cctacgcgaa
      841 ccccttcctg atcctgcata accgctttcc gtggaatacg acggcctacg atggcatagg
      901 cggcaacgac ttcgtgccgg ccgcgacggt ggtggcgctg gagctgcggg tgaacccgtg
      961 gcgctacgcc gaggtgtacg gaacgctcgc gatgaaccag ctgcagtcga gctacgagat
     1021 tgagcgctat ggggccgccg c
//
LOCUS       DAOPHV010000842         1039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_219074_length_1039_cov_3.049797, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000842 DAOPHV010000000
VERSION     DAOPHV010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_219074_length_1039_cov_3.049797"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..540
                     /gene="rpsE"
                     /locus_tag="PLQ29_14815"
     CDS             31..540
                     /gene="rpsE"
                     /locus_tag="PLQ29_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPG87965.1"
                     /translation="MDRRDRDDRSRDIYVEKLVKLNRTAKVVKGGRRFSFSALTVVGD
                     RSGNVGYGFGKANDVTEAIRKSIDKAKRSMVKLPVKNGTIPHDVIGEYKASRVIMMPA
                     CPGSGIIAGGAVRAIMEAGGITDIQAKCVGSRSAVNVIRATFKGLEGIFDARVVAHNR
                     GKALKDMWG"
     gene            543..749
                     /gene="rpmD"
                     /locus_tag="PLQ29_14820"
     CDS             543..749
                     /gene="rpmD"
                     /locus_tag="PLQ29_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPG87966.1"
                     /translation="MAEMIVIKLKKSLIGRKPEQRATVRSLGLRKISSSVVQEATPSI
                     LGMVDAVKHLVEVRPYAPGQEQKK"
     gene            746..>1039
                     /gene="rplO"
                     /locus_tag="PLQ29_14825"
     CDS             746..>1039
                     /gene="rplO"
                     /locus_tag="PLQ29_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPG87967.1"
                     /translation="MSDFNLHPPVGATHRKKIVGRGQGSGLGGTAGKGNKGQQSRSGG
                     KTYPGFEGGQMPLYRRLAHRGFNNKNFRVDFQIVNLNDIEARFEAGEIVDGVSL"
BASE COUNT          226 a          313 c          334 g          166 t
ORIGIN      
        1 aaggccggga tcaaattcta gggggaccat gtggatagga gagacaggga cgacaggtcc
       61 cgcgatatct acgtcgagaa gctcgtcaag ctgaatcgta cggccaaggt cgtcaagggc
      121 ggtaggcggt tttcgttctc cgcgctaacc gtggtgggcg accgctccgg caacgtcggc
      181 tacggattcg gcaaggctaa cgacgtgacc gaggcgatcc gcaagagcat cgacaaggcc
      241 aagaggagca tggtcaagct tcccgtgaag aacggcacca tacctcacga cgtcatcggc
      301 gagtacaaag cctcgagggt catcatgatg cccgcctgcc ccggttccgg catcatcgcc
      361 ggcggcgcgg tccgcgcgat catggaggcc ggcggtatca ccgacatcca ggccaagtgc
      421 gtcgggagcc gctccgccgt caacgtgatc cgcgccacct tcaaggggct cgagggcatc
      481 ttcgatgctc gcgtcgtcgc ccataaccgt ggcaaggcgc tcaaggatat gtggggttaa
      541 tcatggctga aatgatcgtg atcaagctca agaagagcct tatagggcgc aagcccgagc
      601 agcgcgcgac cgtgcgctct ctcggcctgc gcaagataag ctcgagcgtg gtacaggagg
      661 cgactccttc gatactcgga atggtcgacg cggtcaagca cctcgtcgag gtcaggccct
      721 acgcgccggg ccaggagcag aagaaatgag cgacttcaat ctacatcccc ccgtcggcgc
      781 cacgcatcgc aagaagatcg taggccgcgg ccagggctcc ggcctcggag gtaccgccgg
      841 caagggcaat aagggacagc agtcccgctc cggcggcaag acctatccgg gtttcgaggg
      901 cgggcagatg ccgctgtaca ggcggctagc gcacaggggc ttcaacaaca agaacttcag
      961 ggtcgatttc cagatcgtca acctgaacga catcgaagcc cgtttcgagg ccggcgagat
     1021 cgtcgacggc gtctcgctc
//
LOCUS       DAOPHV010000843         1038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_219359_length_1038_cov_5.119023, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000843 DAOPHV010000000
VERSION     DAOPHV010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_219359_length_1038_cov_5.119023"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..>1038)
                     /locus_tag="PLQ29_14830"
     CDS             complement(107..>1038)
                     /locus_tag="PLQ29_14830"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPG87968.1"
                     /translation="SFVVGNYSIESGKLVSYLTGRGALDENERYIIFEVRLPRIVMAS
                     LTGAALAAGGVSMQAIFRNPLVSPFVIGLSSGAALGAAVGIVYLNASVAAVESLSFLF
                     GTVAVFASYLLSGFGRETTLLLLFGLVATSFSHSAIGLMQYFADPEHQLPALTFWMLG
                     GFDGITLSSLKYSVPVILACMAVLYAYSYKMNLLTLSDAEARSLGVNVAAAKFVIIFF
                     STLMVCSCVSKAGVIGWIGLTVPHISRIILGPDNRLVFVSSSILGAAFLLASDDLART
                     LTPGEIPVGIITSAIGAPVFAAILLKYRKSGWN"
BASE COUNT          266 a          281 c          303 g          188 t
ORIGIN      
        1 aaccgcctgg taggagacgc ctccgagggc gaggatcgtc ccggccgcga aggatattta
       61 aacgacagat tttctatctg aattctagcg ttcataaaaa ctccgttcaa ttccatccgg
      121 attttctgta ctttaatagt atggcggcaa agaccggcgc gccgatcgcg gaggttatta
      181 tgccgaccgg aatttcgccg ggcgtgagcg ttcgggccag atcgtccgac gctagcagga
      241 acgctgctcc caggatagaa gacgaaacga aaacgagacg gttgtcgggg cccaggatta
      301 ttcgcgagat gtgcggaacc gtaaggccta tccagcctat gacgcccgct ttcgacacac
      361 acgagcagac catgagcgta ctgaaaaata ttatgacgaa tttcgcggcc gccacgttaa
      421 cgccgagcga cctggcctcc gcgtcgctga gcgtgagcag gttcatttta taagaatacg
      481 cgtaaagaac ggccatgcag gctaatataa ccggaaccga atatttaagc gatgaaagcg
      541 ttatgccgtc gaagccgccg agcatccaga aggtgagcgc cggaagctga tgttcagggt
      601 cggcgaaata ctgcatcagg cctatcgccg agtgggagaa ggaggtggcc accagaccga
      661 agagcagcaa aagcgtcgtt tcgcgtccga aaccggacag caggtacgac gcgaaaacgg
      721 cgactgtgcc gaaaagaaac gaaagcgact ctaccgccgc cacggaggcg ttcagatata
      781 ctatgccgac ggcggcgccc agagcggctc cagatgaaag tcctataacg aaagggctca
      841 ccaggggatt ccggaaaata gcctgcatcg agacgcctcc cgccgcaagg gcggccccgg
      901 ttagcgaggc catcacgatt cgcggcaggc gcacctcgaa tataatgtag cgttcgtttt
      961 cgtccagcgc gccgcggccg gtcaggtatg aaacgagctt tccgctttct atgctgtagt
     1021 tgccgacgac gaacgaaa
//
LOCUS       DAOPHV010000844         1038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_219392_length_1038_cov_3.527976, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000844 DAOPHV010000000
VERSION     DAOPHV010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_219392_length_1038_cov_3.527976"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1038
                     /locus_tag="PLQ29_14835"
     CDS             <1..>1038
                     /locus_tag="PLQ29_14835"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPG87969.1"
                     /translation="TEDATEIPVNAELSITFSKNIDAATVNGSTLPIVDASGTAVGGK
                     YSVSDATVTFAATGGFGYGKSYTVTITSGVLDTDGNPLAEAFSWTFITSLTPDVVAPS
                     LASLSFEAAAAYVDELDRTWLGSLCVELTVAASDDRAVAQVMLSDSPSFLNASWVTFN
                     AAAPVYELVLGGMDGPKRLYGKVKDGAGNVSDVRVSEEVHLDLEPPTIDNLLINGGAS
                     ATNSGLVSLKVFAGDDEGASSGLHRFRYRPANGSWSDWLDLSLDSGAGAGEVSNVATN
                     SELGGSAVFEAQVIDRVGHVSAVASRTIRYEQTPPTVIGVSWDDQALFPYNGSVLRIQ
                     FDEEMRPDSFP"
BASE COUNT          189 a          350 c          327 g          172 t
ORIGIN      
        1 cgaccgagga cgcgaccgag atcccggtca acgccgagct gtcgatcacc ttctccaaga
       61 acatcgacgc cgcgaccgtc aacggctcga cgctacccat cgtcgacgca tcgggaacgg
      121 cggtcggcgg gaaatactca gtttcagacg ccacggttac cttcgccgcg acgggaggct
      181 tcggttacgg caagagctac accgtgacga tcacctccgg cgtactcgac acggacggca
      241 atccgctcgc cgaggcgttt tcctggacct tcatcaccag cctcacgccc gacgtggtag
      301 cgccttccct cgcctcgctg tcgttcgaag cggcggccgc gtacgtcgac gagctcgata
      361 ggacctggct cggctccctc tgcgttgagc ttaccgtggc cgcctcggac gaccgggccg
      421 tcgcgcaggt gatgctatcc gacagcccgt cgttcttgaa cgcctcctgg gttaccttca
      481 acgccgccgc tccggtctat gagttggtac tcggcggcat ggacgggccg aagcgcctct
      541 acggcaaggt caaggacggc gccggcaacg tcagcgacgt acgcgtgtcc gaggaagtcc
      601 acctagatct agagccgccc acgatcgaca acctgctcat caacggcggc gctagcgcca
      661 ccaactccgg cctggtcagc ctcaaggtct tcgcgggcga cgacgagggc gcctcgagtg
      721 gcctccatag gttccgctac cggccggcga acgggagctg gagcgattgg ctcgacctat
      781 cgctcgatag cggcgctggc gctggcgagg tgtccaatgt agccacgaac tccgagctag
      841 gcgggagcgc ggtattcgag gcgcaggtga tcgacagggt gggacacgta tccgcggtag
      901 cgtccaggac gatacggtac gagcagacac ctcccaccgt cataggcgtc agttgggacg
      961 accaggcgct cttcccgtac aacggcagcg tgctgcgcat ccagttcgac gaggagatga
     1021 ggccggacag cttccccg
//
LOCUS       DAOPHV010000845         1037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_219740_length_1037_cov_3.432790, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000845 DAOPHV010000000
VERSION     DAOPHV010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_219740_length_1037_cov_3.432790"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PLQ29_14840"
     CDS             complement(<1..412)
                     /locus_tag="PLQ29_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87970.1"
                     /translation="MRTSCIVIVCAAIAMSCVSAPEPAREPPPASAQQGSSDGSTSQA
                     YKDRAVELVQGGSQGGSTGGAAGSSAAATTGSASGDAVDVPPPAPGVMTPEEEAFLNN
                     YLARLQYMVYYDEAAGIDERNAKVAVNQANRYLLE"
     gene            complement(527..>1037)
                     /locus_tag="PLQ29_14845"
     CDS             complement(527..>1037)
                     /locus_tag="PLQ29_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87971.1"
                     /translation="IFGSPPASKGSALRVDLEAGAFISAVTLEGGGELAVSSAGSITL
                     RGADTGLSVATRSSEAPFRVRPGVARYHISVAQSADGVKVFGSDGASVLVDARLDGAS
                     GWRLWPAVNTSATLAAVSVSAFDPAMVSSVRRDSAAEGLSMLDSRAEATPTGRLSIAV
                     ETPSVLANL"
BASE COUNT          200 a          329 c          352 g          156 t
ORIGIN      
        1 tctcgagcag gtaccggttg gcttgattga cggcgacctt ggcgttgcgt tcgtcgatgc
       61 cggcagcctc gtcgtagtag accatgtact ggagacgggc caggtagttg ttcaggaagg
      121 cttcctcctc gggggtcatc accccggggg ccggaggcgg cacgtcgacg gcgtcgcccg
      181 aggcggaacc cgtggtcgcg gcggccgagg aaccggcggc gccgccagtc gatccgcctt
      241 gagagccgcc ctgcacgagc tcgacggccc tatccttata agcctgcgac gtactcccgt
      301 cggacgagcc ttgctgggcc gacgcgggcg ggggctctcg agccggctcc ggagcgctga
      361 cgcatgacat ggctatggcg gcgcagacga tgacgataca cgatgttctc atacggccct
      421 tccacaaata cggtaaggtt actatagcgc gctcgccgcg ctaaaacagc acggtaccaa
      481 tggtagtcaa ttactagaaa tattcaaggc tcggggaaga aaagggttac agattcgcca
      541 ggaccgacgg cgtctcgacg gcgatggata gcctgccagt cggagtagcc tcggcgcgag
      601 agtcgagcat ggataggccc tcggccgcag agtcgcggcg gacggacgat accatggcag
      661 ggtcgaaggc cgagaccgac acggcggcca gggtcgccga cgtattcaca gcaggccaca
      721 ggcgccatcc agaagcgccg tcgagacgag cgtccacgag taccgaggcg ccgtcggagc
      781 cgaagacctt gacgccgtcc gcggactggg cgaccgatat atggtagcgc gccacgccgg
      841 gcctgacacg gaacggcgct tccgaggacc tggtcgccac ggacaagccg gtatccgcgc
      901 cacgtagcgt tatgctccct gctgacgaaa cggccagctc cccaccgccc tcgagcgtca
      961 cggcggatat gaacgcgccc gcctcgaggt cgacgcgcag cgctgagcct ttcgaggccg
     1021 ggggcgagcc gaaaatc
//
LOCUS       DAOPHV010000846         1037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_219741_length_1037_cov_3.416497, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000846 DAOPHV010000000
VERSION     DAOPHV010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_219741_length_1037_cov_3.416497"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLQ29_14850"
     CDS             <1..540
                     /locus_tag="PLQ29_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HPG87972.1"
                     /translation="RIDKVIGNDKLSPVIQSLGCGIGADFDVSKLRYHKVIIMADADV
                     DGSHIRTLLLTFFFRYMTDLLRRGHVYLAMPPLYKIVIDRKIQYAYDDAEKDRILAES
                     GKDPDKVAIQRYKGLGEMNPDQLWETTMDPEHRNIIQVHMEDLVEAETVFSTLMGEQV
                     EPRRRFIEENALSVSRLDV"
     gene            542..>1037
                     /locus_tag="PLQ29_14855"
     CDS             542..>1037
                     /locus_tag="PLQ29_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPG87973.1"
                     /translation="MAELTGKIIPVAVEEEVRTAYLTYAMSVIVSRALPDARDGLKPV
                     HRRILYAMDELGLKPTAATKKSARITGDAMGKYHPHGDLSLYDALVRMAQTFSLRYPL
                     ITGQGNFGSVDGDPPAASRYTEAKLSKVGDELLADLEKETVEFVPNYDESLKEPSVLP
                     AAVPN"
BASE COUNT          225 a          333 c          317 g          162 t
ORIGIN      
        1 cgcatcgaca aggtgatagg caacgataag ctcagcccgg tcatccagtc gctcggctgc
       61 ggcataggcg ccgacttcga cgtgtcgaag ctacgctacc acaaggtcat catcatggcc
      121 gatgcggacg tcgacggctc gcatatccgc accctgctcc tgaccttctt cttcaggtac
      181 atgaccgacc tcctgcggcg cgggcacgtg tacctcgcga tgccgccgct gtacaagatc
      241 gtgatcgacc gcaagatcca gtacgcctac gacgacgccg agaaggatcg catcctcgcg
      301 gagagcggca aggatcccga caaggtggcc atccagcgct ataagggctt gggagagatg
      361 aacccggacc agctatggga gacgacgatg gaccccgagc accggaacat catccaggtg
      421 catatggagg acctcgtgga ggccgagacg gtcttctcga cgctcatggg cgagcaggtg
      481 gagccccgaa ggcgatttat agaggagaac gcgctgtcgg tctcccgctt ggacgtataa
      541 catggcagaa ctaacaggaa agatcatacc cgtagcggtc gaggaagagg tccggaccgc
      601 ctacctgacc tatgcgatga gcgtcatcgt cagccgcgcc ttaccggacg cgcgagacgg
      661 cctcaagccg gtgcataggc gcatcctcta cgcgatggac gagctcgggc tcaagccgac
      721 cgccgccacc aagaagagcg cccgcatcac cggcgacgcg atgggaaagt accacccgca
      781 cggcgaccta tcgctgtacg acgccttggt acgcatggcc cagaccttca gcttgcgcta
      841 cccgcttatt accgggcagg gaaacttcgg ctcggtcgac ggcgatccgc ccgccgcgag
      901 ccgctatacc gaagccaagc tgtcgaaggt cggcgacgag ctgctggccg atctcgagaa
      961 ggagacggtc gagttcgttc ccaactacga cgagagcctc aaggagccct cggtcctgcc
     1021 ggcggccgtg ccgaacc
//
LOCUS       DAOPHV010000847         1034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_220893_length_1034_cov_2.569969, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000847 DAOPHV010000000
VERSION     DAOPHV010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220893_length_1034_cov_2.569969"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1034)
                     /locus_tag="PLQ29_14860"
     CDS             complement(<1..>1034)
                     /locus_tag="PLQ29_14860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG87974.1"
                     /translation="HEAAVEDALFEQEHARSIVKAAKEDNERKTIRIRSDKLDGLLNL
                     VGELVTVQAQLSQTVRDSTDRHLHGISDYVKRLAGELRATSMELRLVPVESLFSKFKR
                     LARDLSKNLGKEFEIRFEGGDTEIDKSVIDVLNDPLVHLVRNAADHGLESTDDRAALK
                     KPRCGTITMKAQHAGAFVRIGVADDGRGLNREKILKKAIDRGIVPPDAKLADEEIDQL
                     IFKPGFSTADVISNVSGRGVGLDVVKTAIDQLGGTLNVKSRIGQGTEFCLDIPLTLAI
                     IDGFLVRSGRRMFVIPLSSVDSCFERERPAEPERLIVRGKDFIPYVSSRELFGEDEKP
                     PLREEVVIVS"
BASE COUNT          156 a          330 c          342 g          206 t
ORIGIN      
        1 gctgacgatg acgacctcct ccctgagcgg cggcttctcg tcctcgccga acagctcgcg
       61 cgacgatacg tagggtatga agtccttgcc cctgacgatc aggcgctcgg gctcggccgg
      121 gcgctcccgc tcgaagcacg agtcgaccga cgatagcggg atgacgaaca tgcgccgccc
      181 ggatcgcacg aggaagccgt cgatgatcgc gagggtcagc ggtatgtcca ggcagaattc
      241 ggtgccctgg ccgatccgag acttgacgtt tagcgtgccg ccgagctggt cgatggccgt
      301 cttgaccacg tcgaggccga ccccacggcc cgagacgttg gagatcacgt cggcggtcga
      361 gaagcccggc ttgaagatca gctggtctat ctcctcgtcg gcgagcttgg cgtccggcgg
      421 tactatgccg cggtcgatcg ccttcttgag tatcttctcg cggttcaggc cgcggccgtc
      481 gtccgccacg ccgatgcgca cgaaggcgcc ggcatgctgg gccttcatgg tgatcgtgcc
      541 gcagcgaggc ttcttaagcg cggcgcggtc gtcggtcgat tctaggccgt ggtcggccgc
      601 gttgcgcacc aggtgcacga gcggatcgtt caggacgtct atcaccgact tgtctatctc
      661 ggtgtccccg ccctcgaagc ggatctcgaa ctccttgccc aggttcttgg acaggtcgcg
      721 ggccaggcgc ttgaacttgg agaacaggga ctcgaccggt acgagcctga gctccatcga
      781 ggtggcccgt agctcgccgg caaggcgctt cacgtagtcg ctgatgccgt gcaggtggcg
      841 gtctgtcgag tccctgacgg tctgggaaag ctgggcctgt acagtgacca gctcgccgac
      901 caggttgagc aggccgtcga gcttatccga gcgtattcgt atggtcttgc gctcgttgtc
      961 ctccttggcg gccttgacga tgctccgcgc gtgctcctgc tcgaacagcg catcctcgac
     1021 cgcggcttcg tgct
//
LOCUS       DAOPHV010000848         1032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_221609_length_1032_cov_2.342886, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000848 DAOPHV010000000
VERSION     DAOPHV010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221609_length_1032_cov_2.342886"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..585
                     /locus_tag="PLQ29_14865"
     CDS             136..585
                     /locus_tag="PLQ29_14865"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPG87975.1"
                     /translation="MDRTKRSVDAGLLEALRDALSPVASIRLVVLFGSRARGEARPDS
                     DVDVAVSFGSTPSLLDFGDVVSRCEAATGLNVDVVELDGLPSSKPALAYEIARDGTVV
                     LDRDDGFWRYRARAFSLYLDQRDFLERQRALFSDRLDEGNLGMAKHA"
     gene            578..973
                     /locus_tag="PLQ29_14870"
     CDS             578..973
                     /locus_tag="PLQ29_14870"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPG87976.1"
                     /translation="MLERLAQLDQNVSSLEMLRAENAPEAFGSSITLQWALRHGLIES
                     IQIVIDTACEVCSKNNLGVPSSYGDCVRLLARARYLSSDCAEELVKVIGLSNLLIHEY
                     TAIDTGRLYGFLAQLDIFRRYAKELRPYL"
BASE COUNT          197 a          319 c          311 g          205 t
ORIGIN      
        1 aaccctgcat ctaccgaacg cttagtccta tccacgatag cgcccccgta cccgtccacg
       61 aatccttacg cgccagccgc gccgacgatc acgcgggaca ggctcacctc cgtagccggg
      121 tacgggggcg ctatcgtgga taggactaag cgttcggtag atgcagggtt gctcgaagcc
      181 cttcgcgacg ctctctctcc cgtggcgtcg atacggctcg tcgtgctgtt cggttcgcgg
      241 gcccgtggcg aagcgcgacc cgatagcgac gtcgacgtgg ccgtatcgtt cggatcgacg
      301 ccgtcgctcc tggacttcgg ggacgtcgtg tcgagatgtg aagccgccac ggggctgaac
      361 gtagacgtcg tcgagctcga cggcctcccc tcgtcgaaac cggccttggc ctacgagata
      421 gcccgggacg gaaccgtcgt cctcgaccgc gacgacggtt tctggcgcta tagggcgagg
      481 gcgttcagcc tctatctgga ccagcgagac ttcttggaac ggcaacgggc ccttttctcg
      541 gatcgtcttg acgaagggaa cttagggatg gcgaagcatg cttgagagat tggcgcagct
      601 agaccagaac gtttcaagcc tcgagatgct acgagcagag aacgccccgg aagcgttcgg
      661 ttcctcgata accctacaat gggcgcttcg ccatggcttg atagagtcga tccagatcgt
      721 catcgatacg gcctgcgagg tttgctctaa gaacaacctg ggcgtaccgt ctagctacgg
      781 cgactgcgtc cgattgctcg cgcgagcgcg atacctaagc tccgactgcg ccgaagagct
      841 ggtcaaggtc atcggcctca gcaatctcct tatccatgag tatacggcta tcgatacggg
      901 gcgcctctac ggcttcctgg cccaactaga tattttcagg cgatacgcga aggagcttag
      961 gccataccta tgagccagac gatctcgttg acgaccatca tcctctgcgg cggcgccggc
     1021 acgcggctat gg
//
LOCUS       DAOPHV010000849         1030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_222379_length_1030_cov_1.836923, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000849 DAOPHV010000000
VERSION     DAOPHV010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_222379_length_1030_cov_1.836923"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLQ29_14875"
     CDS             <1..309
                     /locus_tag="PLQ29_14875"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPG87977.1"
                     /translation="PLDKAIPCGLVVNELVANAMERAAPDRPVDTVTVEFRRADSAWR
                     LSVSDDGPAGDDGPKADATAGACLDLQLAEGLVAQLGGVIRREPLPDGGTLVIVDFPA
                     "
     gene            467..>1030
                     /locus_tag="PLQ29_14880"
     CDS             467..>1030
                     /locus_tag="PLQ29_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87978.1"
                     /translation="MKRTLVALLLVAALAAPAFGQDVGAPWIDSGLPFAAAYDGTTAS
                     PTFAVALPTSILAFISNGIYADEIDMVVRNPAALSQFSGYTLWTLYGNYEAFNNAATL
                     NTPQAITGAEVQPFNTTTLNDANIGNFQAGIGMPVPFMDGWRMGLLGGRQFTRANGLT
                     GGYSTYDTSDKPTLTTVDADADGTADAT"
BASE COUNT          187 a          376 c          289 g          178 t
ORIGIN      
        1 cccctggata aggccattcc ctgcggcctc gtcgtcaacg agctggtcgc gaacgccatg
       61 gagcgcgccg caccggaccg ccccgtcgat accgtcaccg tcgaattccg ccgcgccgac
      121 tccgcgtggc gcctgtccgt ctccgacgac ggccccgccg gcgacgacgg ccccaaagcg
      181 gacgccaccg ccggcgcgtg cctcgacctc cagctcgccg agggcctcgt cgcccagctc
      241 ggtggcgtca tccgccgcga gccgctcccg gacggcggca ccctcgtcat cgtcgacttc
      301 ccggcctagt cccgtcagaa aaggttacag aatccgggct tttgtcacga ttttttatta
      361 ataattttac attgcccata cagtgagcta tactccctat ggcgccgtcg gcgcgggagc
      421 cccaggctcc gctcatgcat tgcgcaccta taaggaaagg actcttatga aacgcacatt
      481 agtagcgctc ctgctcgtgg ccgccctcgc ggcgcccgcg ttcgggcagg atgtcggagc
      541 gccctggata gactcaggcc ttccgttcgc ggccgcgtat gacggcacga cagcctcgcc
      601 taccttcgcg gtggccctgc ccacctcgat cctggccttt atctccaacg gcatctacgc
      661 ggacgagata gacatggtcg tacgaaaccc cgcagcgtta agccagttca gcggctacac
      721 gctctggacg ctgtacggta actatgaggc gttcaacaac gccgcgaccc ttaatacgcc
      781 ccaggcgatt acgggcgcgg aagtccagcc gttcaatacc acgacgctga acgacgcgaa
      841 tatcggcaac ttccaggccg gtatcggcat gccggtaccg ttcatggacg gctggcgcat
      901 gggcctcctc ggcggcaggc agttcaccag ggccaacggc ttgaccggag gctactcgac
      961 ctacgatacc tccgacaagc ctaccctcac gaccgtagac gccgacgccg atggcaccgc
     1021 cgacgccacc
//
LOCUS       DAOPHV010000850         1029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_222468_length_1029_cov_4.113963, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000850 DAOPHV010000000
VERSION     DAOPHV010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_222468_length_1029_cov_4.113963"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PLQ29_14885"
     CDS             complement(<1..207)
                     /locus_tag="PLQ29_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPG87979.1"
                     /translation="MSGLEDRTARIIICEDERIVALDLRAFLQRNGYEVPSLFASAED
                     LLESVEALEPDLVLMDIHLQGAMDG"
     gene            complement(262..630)
                     /locus_tag="PLQ29_14890"
     CDS             complement(262..630)
                     /locus_tag="PLQ29_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPG87980.1"
                     /translation="MAKSILIIDDSAAIRQSISYILQQEGYATVEAGDGVEGLQRLDE
                     GTRFDCIITDVNMPNMDGITFIRKARENPKFKFVPILVLTTESQGGKMNEGKEAGATG
                     WIVKPFSADKLLAVVKKVAE"
     gene            complement(702..>1029)
                     /locus_tag="PLQ29_14895"
     CDS             complement(702..>1029)
                     /locus_tag="PLQ29_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HPG87981.1"
                     /translation="LLKRSGARYYVEVKEGPLVCRHRPSVDVLFRSAARYAGNNAVGV
                     IMTGMGDDGSRGMKEMHDAKAWTIAQDEQSCVVFGMPNEAIKLGGVDVVLPLGKIASE
                     VLRAAK"
BASE COUNT          172 a          343 c          302 g          212 t
ORIGIN      
        1 gccgtccata gcgccctgca ggtggatatc catcagcacg aggtccggtt ccaaggcctc
       61 gacggactcg agcagatcct cggccgaggc gaagagggac ggcacctcgt agccattacg
      121 ctggaggaag gcgcgaaggt cgagggcgac gatgcgctcg tcctcgcaga tgattatacg
      181 cgctgtgcga tcctcgaggc cgctcatccg cgcctcgagg cgttccccgc gctcggcgga
      241 cgcggagtcg gcgtacgcat gctactcggc gaccttcttg acgacggcca gtagcttatc
      301 ggcgctgaac ggcttgacta tccaaccggt agcgccggct tccttgccct cgttcatctt
      361 gccgccctgc gactcggtgg tcagcacgag gatcggcacg aacttaaatt tcgggttctc
      421 cctggccttg cggatgaagg tgatgccgtc catgttcggc atgttgacgt cggtgatgat
      481 gcaatcgaag cgggtgccct cgtcgagccg ctggagccct tcgacgccgt ctcccgcctc
      541 taccgtagcg tagccttcct gctgcaggat ataggaaatg ctctgccgta tggcggccga
      601 atcgtcgata atcaagatac tcttcgccat gatctctcct cgggccgcgc ggctcgacgc
      661 cggaccctta tgtattgcta gcgccgttca aagcgaacgc gttacttggc cgccctcaag
      721 acctcggacg ctatcttgcc gaggggcaac accacatcca cccccccgag cttgatcgcc
      781 tcgttcggca tgccgaagac cacgcatgac tgctcgtcct gggctatagt ccaggccttc
      841 gcgtcgtgca tttccttcat gcctcgcgag ccgtcgtccc ccatgccggt catgatcacg
      901 ccgacggcgt tattgccggc gtacctggcc gccgacctga acagcacgtc gacgctcggc
      961 ctgtgcctgc ataccagcgg gccttccttg acctcgacat agtagcgggc gccggatcgc
     1021 ttgagtagt
//
LOCUS       DAOPHV010000851         1029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_222539_length_1029_cov_2.714579, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000851 DAOPHV010000000
VERSION     DAOPHV010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_222539_length_1029_cov_2.714579"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PLQ29_14900"
     CDS             <1..397
                     /locus_tag="PLQ29_14900"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPG87982.1"
                     /translation="FVLVVMVGILNTYRVIVYERTREIGTMRALGMSSGAVSRLFVVE
                     ALGLALLSSAGGLVAALVLFKLAGFIDLSSVPSAGLFTERGRLRFYIDARIMTLNLAI
                     MVGGVVLAALGPARKASLIHPADAMRDEA"
     gene            454..>1029
                     /locus_tag="PLQ29_14905"
     CDS             454..>1029
                     /locus_tag="PLQ29_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPG87983.1"
                     /translation="MNRYRLPIALSIALAALGSVQAQGTDYSELLKRVDATVTFEGSN
                     LSAEYTIVKRDPGGATSTTVATMFRRDATGQFLILILAPAIDKGKGYLKEGDNLWLYD
                     PVDRVFTFTSAKERFQNSSFRNSDFNRSNFSKDYTPVSGTREKLGKFECTVLELRATN
                     DTVSFPRMRIWVTDDYLVRKIEDYSLSGQLMR"
BASE COUNT          198 a          298 c          307 g          226 t
ORIGIN      
        1 cttcgtgctc gtggtgatgg tcgggattct gaatacctac cgcgtgatcg tctacgagcg
       61 tactcgcgag ataggtacga tgcgcgccct gggcatgagt tcgggcgccg tgtctcgtct
      121 cttcgtcgtc gaggcgctcg ggctagccct gctgtcgtcg gccggaggcc tggtcgcggc
      181 actcgtcctg ttcaagctag ccggattcat agacctgtcg agcgtaccgt cggctggcct
      241 ctttaccgag agggggaggc tacgcttcta tatcgacgcg cgcatcatga ccttgaacct
      301 ggccatcatg gtcggcggcg tcgtgctggc ggccctggga cccgcgcgca aagcctcctt
      361 gatccacccg gcggatgcga tgcgcgacga ggcttgaaac cggaaggccc gggcgtttgt
      421 tacgatagta cattccgtta gtaaaggatg ctcatgaaca gatatagact gcctatagcc
      481 ttgagtatag ccttggcggc ccttggctcc gtacaggcgc agggtacgga ctatagcgag
      541 ctcctcaagc gagtggacgc caccgttacc ttcgagggat ctaacttgtc cgccgagtac
      601 accatcgtca aacgcgatcc cggcggggcg acgagtacga cggtggccac gatgttccgc
      661 cgcgatgcca ccggccaatt cctgattctg atcctggcgc cggccatcga caagggcaag
      721 ggctacttga aggaaggcga caacctttgg ctctacgacc ccgtggaccg ggtctttacc
      781 tttacgagcg ccaaggagcg tttccagaat tcaagcttca ggaattctga tttcaatcgg
      841 tctaatttct ccaaggacta tacgcccgtt tccgggacga gggaaaagtt aggcaagttc
      901 gaatgcacgg tgctcgagct ccgggccacc aacgatacag tatcctttcc tcgcatgcgt
      961 atctgggtaa ccgacgatta cctggtccgt aagatcgagg actatagctt gtccggtcag
     1021 ctgatgcgc
//
LOCUS       DAOPHV010000852         1023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_224590_length_1023_cov_3.474174, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000852 DAOPHV010000000
VERSION     DAOPHV010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_224590_length_1023_cov_3.474174"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PLQ29_14910"
     CDS             complement(<1..452)
                     /locus_tag="PLQ29_14910"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87984.1"
                     /translation="MEPTTIIHPATGFAIGDPYTDPLATRPTPEKARAFFDTLILSAS
                     GWRGVFGSSDDDLGETITPAAAYAVARMADTFAGFLIRGGAGAGAPVRGPVVALGVDT
                     RPTGPAIADVMARVFLARGLSVRYAFVCAAPEIMAWVRRAGALPADSP"
BASE COUNT          192 a          313 c          337 g          181 t
ORIGIN      
        1 tcggggctgt cagctggcag ggccccggcg cgcctgaccc aggccatgat ctccggcgcg
       61 gcgcagacga aggcgtagcg caccgacagg cctcgggcca ggaagacgcg ggccatcacg
      121 tcggcgatgg ccggcccggt cggcctcgtg tcgacgccga gcgcgacgac gggcccccgg
      181 acgggcgccc cggctcccgc ccctccgcgt atgaggaagc cggcgaaggt atcggccatc
      241 ctggcgacag cgtaggcggc cgcgggcgtg atagtctcgc ccaggtcgtc gtccgacgag
      301 ccgaacacgc cgcgccagcc ggacgccgac aggatgagcg tatcgaagaa ggcgcgcgcc
      361 ttttcgggcg tcggcctcgt agcgagagga tcggtatatg ggtcgcctat cgcgaagccc
      421 gtggcgggat ggatgatcgt tgtaggttcc atgcgcgtat tatagtaaaa aacgatgccg
      481 aggaaagacc ccgatccgca cgggccgttc taggctccgt cacgcgaagg cgccaaggca
      541 cggaggacgt ggcatacgcg aagacgagat cttcttaggc aaggcctcac accaaggcac
      601 gaagggatag ggttaagacg gaacacgatg atcaggatgg cgcaggatga ccaagaagaa
      661 cgctaaggtc ttgtttattt cccttgtaca tcgtgtcttc atcttgtcat cctgttcgta
      721 ttcgacaatc cgccgccggg ctcagtcgct cgggagccat gttccgtcgc cgggagccat
      781 gttccgtcgc cgaggagcca tgttccgtcg ccgaggagcc atgtttcgtc gccgggagcc
      841 atgttctgtc accgaggagc catgttccgt caccgggagc catgtttcgt caccgggagc
      901 catgtttcgt cgctcgggaa ccatggttcg tcgccgagga accatgttcc gtcgccgagg
      961 aaccatgttt cgtcaccgaa gaaccatgtt tcgtcaccga agaaccatgt ttcgtcgctg
     1021 gga
//
LOCUS       DAOPHV010000853         1021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_225427_length_1021_cov_2.879917, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000853 DAOPHV010000000
VERSION     DAOPHV010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_225427_length_1021_cov_2.879917"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PLQ29_14915"
     CDS             complement(<1..473)
                     /locus_tag="PLQ29_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87985.1"
                     /translation="MKPLPESESATSVKALAPVSRQRARYLALVGLSSALLVARVALT
                     SPSFDAVAVARAAVSGLALVVVGILTVDASLNKKRPLVARLSSKSRRLAAAAYLTTYA
                     VCVLDVAAGVDPYLFIPGMVVYCFFVWDRPGFLAVSAGLCAYAVTRAMAAFGRGVG"
     gene            complement(642..>1021)
                     /locus_tag="PLQ29_14920"
     CDS             complement(642..>1021)
                     /locus_tag="PLQ29_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87986.1"
                     /translation="MKLAEAHGADAVFAPEPAALDGVGPAVYGASLSLGWEPPTASAS
                     FAELVIARKLSLAAFADAAWAGSPAGLAVPALPAALTIGLRAGCDFSLIGLKSSSLSL
                     EAGYDLAGGSFAARLYLSPVGGY"
BASE COUNT          192 a          342 c          359 g          128 t
ORIGIN      
        1 ccgacgcctc gcccaaaggc cgccatggcc cgcgtcacgg cataggcgca tagccccgcg
       61 gatacggcca ggaagccggg ccggtcccag acgaagaagc agtagacgac catccccggt
      121 atgaacaggt acggatcgac gccggcggct acgtcgagga cgcagacggc gtaggtggtc
      181 aggtaggcgg cggccgcgag gcgacggctc ttggacgata ggcgggcgac gagcggccgc
      241 ttcttgttga gcgacgcgtc gaccgtcaag atcccgacga caaccagggc caatccggat
      301 acggcggccc gcgcgacggc gacggcatcg aaggacggcg aggtcaacgc gacccgcgcg
      361 acgaggagag cgctcgacag gcctacgaga gcgaggtagc gggctcgctg acgagacacg
      421 ggggcgagag ccttgaccga cgtcgcgctt tccgattcag gcaatggttt cataaatgat
      481 cagccttttt ctattcgtgt catgataacg gcggtcttcg acggcgagac gtgagcgagg
      541 cggccgttgc accgaggagc catcgaaacc ctccgcatac gagtaccgtt taaccgtaag
      601 cgccttgccc ccgcttgtca accttcgcaa cgtcgccggg ctcagtaccc cccgaccggc
      661 gacaggtaca gccgagcggc gaacgagccg ccggccaggt cgtagcctgc ctcgagtgac
      721 aggctcgacg acttgagccc gatcagcgag aagtcgcagc cggcccgcag cccgatggtc
      781 agcgccgccg ggagcgccgg gacggcgagg ccggcagggc tgccggccca ggcggcgtcc
      841 gcgaaggccg ccagcgacag cttcctggct atgacgagct cggcgaacga ggccgaggca
      901 gtcggaggct cccagcctag gctcagcgac gccccgtaga ccgctggccc gacgccgtcg
      961 agggcggccg gctcgggcgc gaacacggcg tccgcgccgt gcgcctcggc aagcttcatg
     1021 t
//
LOCUS       DAOPHV010000854         1021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_225479_length_1021_cov_2.537267, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000854 DAOPHV010000000
VERSION     DAOPHV010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_225479_length_1021_cov_2.537267"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PLQ29_14925"
     CDS             <1..834
                     /locus_tag="PLQ29_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87987.1"
                     /translation="DVETGFLHPGPDGGQDIAINGYEVWRSANGGDYERIARYYYDTA
                     TSGTHTYYDVDPSLELGVMYSYKVRAYCGNAISAFSDVAQAQFVKSFRTSLDAAYNED
                     WGSSSPGSGVISTKAVNLEFAVSDSSILSTSSPIADEFGFLMVIKEKNGQFLAYLDFL
                     YDITNRLGNGTGKFYYRSVNGSTWYSAGNAVQKYDQVYIDIYSGSFGISHNPGVTYEW
                     NIYGRYLGGGNFAQSPNMSPSYLKKYFTNGEAVSYSDIYQNGNDTSNGWYSYTIADDA
                     E"
BASE COUNT          243 a          300 c          282 g          196 t
ORIGIN      
        1 gatgttgaga cagggtttct ccatccggga ccggatggcg ggcaggacat cgccatcaac
       61 ggatacgagg tctggcggtc agccaacggc ggagactatg agcgcatagc gcgctactac
      121 tacgacaccg ccacgagcgg cacccatacc tactacgacg tcgacccgag ccttgaatta
      181 ggcgtcatgt actcgtataa agttcgcgcc tactgcggca acgccatctc cgcgttctcg
      241 gacgtggcgc aggctcagtt cgtcaagagc ttcaggacca gcctcgacgc ggcatataat
      301 gaagactggg ggtcatctag cccaggatcc ggagtcatta gcacgaaagc cgtaaaccta
      361 gagttcgcgg taagcgactc cagcatccta agtacatctt caccgatcgc tgacgaattc
      421 ggattcctga tggttatcaa ggagaagaac gggcagttcc tggcctatct ggatttcctc
      481 tatgacatca cgaacaggct aggcaacgga accggcaagt tctattaccg tagtgttaat
      541 ggtagcacct ggtatagcgc ggggaacgct gtccagaagt atgaccaggt atacatcgac
      601 atttacagcg gctccttcgg cataagccat aatcccggcg tcacctatga gtggaacatc
      661 tacggaaggt acctcggagg tggcaacttc gcccaatcgc cgaacatgtc gccgagctac
      721 ctcaagaagt acttcaccaa cggcgaggcc gtctcgtact cggacatcta ccagaacggc
      781 aacgacacgt ccaacggctg gtattcctat accatagccg acgacgcgga ataaggaggc
      841 gaacatgaag acactcaagc tgtttttagg cgcctccctc gccctgtgcg tggccgcgtc
      901 gtgcgcgaac ccgtcgctca acgtgaatcc ggcatccgga gggagcgcgt cagcgcaggc
      961 tgataccgtc aaggacgccg tcaagatcgt aggcgtaccg tcgcccgtcc cggctgagct
     1021 c
//
LOCUS       DAOPHV010000855         1021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_225519_length_1021_cov_2.307453, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000855 DAOPHV010000000
VERSION     DAOPHV010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_225519_length_1021_cov_2.307453"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PLQ29_14930"
     CDS             complement(<1..571)
                     /locus_tag="PLQ29_14930"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPG87988.1"
                     /translation="MDRKQAKKERTRRFFLDAAKGIIRAEGVDSLTTKKIGEKAAYSY
                     ASIYNYFENFNGLVCECLEELARESADWVAARIKGETPAERVVDFARLMIEANAREPN
                     VYSVFLSTNIDYGYFARRDGRPFVHPAYPLLLAELERFPELSSGGERGEARVLADILT
                     YVFHSKLHFYIRYGTPTSLKRLHAEVEEEV"
     gene            750..>1021
                     /locus_tag="PLQ29_14935"
     CDS             750..>1021
                     /locus_tag="PLQ29_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87989.1"
                     /translation="MTDRSGTIFGNAMGAAVIRKAERAKASYLREFGDDSGKPLRLGA
                     RPVPALADLGVDELTVSEGGSLDAGIDLRADERALVIGTIRMGFGH"
BASE COUNT          197 a          309 c          329 g          186 t
ORIGIN      
        1 gcacctcctc ctccacttcg gcgtggagcc gcttaaggct ggtcggggtg ccgtagcgga
       61 tgtagaagtg cagcttcgag tggaagacgt aggtgaggat atccgcgagc acgcgcgcct
      121 cgccccgctc cccgcccgac gatagttcag ggaaacgctc gagctccgcc agcagcaacg
      181 ggtaggccgg gtggacgaag gggcggccgt cccgcctcgc gaagtagccg tagtcgatgt
      241 tggtggacag gaacaccgaa tagacgttgg gctcgcgggc gttcgcctcg atcatcaggc
      301 gggcgaagtc gacgacgcgc tcggccgggg tctcgccctt gatccgggcg gccacccaat
      361 cggcgctttc gcgggccagc tcctcgaggc actcgcagac gagcccgttg aaattctcga
      421 agtagttata gatggacgcg taggagtacg ccgccttctc gccgatcttc ttcgtcgtga
      481 ggctatcgac gccctccgcc cggatgatgc ccttcgcggc gtccaggaaa aagcgcctcg
      541 tccgttcctt cttcgcctgc ttcctgtcca tcgagcctcc gttcaattta tgaatgccat
      601 taaataacag ttcgcgcgtc gatgcaagct tcggcttgac agttcggtat aacggccata
      661 aagtattaat ggctttcagt ttttgaacgc aacaaatatt ccagatcgaa gacgggcctc
      721 gagactatag cgacgcggga gcgagcacga tgacggatag aagcggaacg attttcggca
      781 acgcgatggg ggccgcggtc atacgcaagg cggaacgcgc caaggcatcc tacctcaggg
      841 agttcggcga cgactcggga aagccgctcc gactcggggc caggccggtg ccggcgctcg
      901 ccgacctcgg cgtagacgag ctcacggtga gcgaaggcgg atcactggac gcgggcatcg
      961 acctgcgcgc cgacgagcgg gccctggtga tcggcacgat acgcatgggc ttcggccatt
     1021 a
//
LOCUS       DAOPHV010000856         1020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_225791_length_1020_cov_2.675648, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000856 DAOPHV010000000
VERSION     DAOPHV010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_225791_length_1020_cov_2.675648"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PLQ29_14940"
     CDS             <1..571
                     /locus_tag="PLQ29_14940"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPG87990.1"
                     /translation="FYRVEEAPSRPDLASAELLKSVSDYMNRNEKGRIEDWAIARATS
                     FAAAAKADFAAAVAAAGYSVKATSPFPLNYGDALDIGYFSLLGSLDAETLPELRGADS
                     NERFLQAVFSLEAGEVSAPVVHNDNAIVIRVKEINTAADGELDLLEAYYPMVVQQNAS
                     SELASAILADPLLKDDFIAAFSRAFAQAD"
     gene            580..>1020
                     /locus_tag="PLQ29_14945"
     CDS             580..>1020
                     /locus_tag="PLQ29_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87991.1"
                     /translation="MTGSEPLLVDTGRGFSIRSGARWLYSSRAPETAPVEAARTATIL
                     PETLYLIPSPCLWYGAAELLERLPPSSALLGVEAVPELLALAASSLAKSGLEGLSGGR
                     VALARCSGAVAAYRGMEAALGLRFGRVVVVRLSAGDRLDEAAYRA"
BASE COUNT          153 a          380 c          320 g          167 t
ORIGIN      
        1 cttctaccgc gtggaggagg cgccgagccg gcccgacctc gcgtccgcag agctcctgaa
       61 gtccgtcagc gattacatga accgtaacga gaagggccgt atcgaggact gggccatcgc
      121 gagggcaacc tcgttcgccg cggccgccaa ggccgacttc gccgcggccg tcgccgccgc
      181 cggctactcg gtcaaggcta cgtcgccctt cccgctcaac tacggcgacg ccctcgacat
      241 cggatacttc tccctgctcg gctccctcga cgccgagacc ctgccggagc tacgcggcgc
      301 cgactcgaac gagcgcttcc tccaggcggt gttctccctc gaggccggcg aagtctcggc
      361 gccggtggtc cataacgaca acgccatcgt catcagagtc aaggagatca acaccgccgc
      421 cgacggcgag ctagacctcc tcgaggccta ctatccgatg gtcgtccagc agaacgcctc
      481 atccgagcta gcctcggcca tcctggccga cccgctcctg aaggacgact tcatcgcggc
      541 gttctctcgg gccttcgccc aggccgacta gggccgtcca tgaccggctc cgaacccctg
      601 ctcgtcgata cgggcagggg tttttctata cgctccggcg ccagatggct ctactcgagc
      661 agggctcccg agaccgcccc cgtcgaggcg gccagaacgg caaccatcct acccgagacg
      721 ctatacctga tcccttcgcc atgcctctgg tacggcgccg ccgagctcct cgagaggcta
      781 ccgccgtcga gcgccctgct cggcgtcgag gccgtgcccg agttgctcgc tctggcggcc
      841 tcgagcctgg ccaagagcgg cctagaaggt ctatctggcg gcagggtcgc cctggcccgc
      901 tgttcgggcg cggtcgccgc gtaccgcggt atggaggccg cgctcggcct gcgtttcggg
      961 cgcgtcgtcg tcgtgcggct atcggctggc gacaggctcg acgaggcggc ctaccgggcg
//
LOCUS       DAOPHV010000857         1018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_226451_length_1018_cov_3.960540, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000857 DAOPHV010000000
VERSION     DAOPHV010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_226451_length_1018_cov_3.960540"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PLQ29_14950"
     CDS             complement(<1..504)
                     /locus_tag="PLQ29_14950"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPG87992.1"
                     /translation="MDMKRIGCIVALALLAGSAFAQSSEDEARRRLVDAAFKYRGVPY
                     VYGAESPRAFDCSGFVRYVYREALGLELPRSARSFWSSGSPIDWRKARAGDVLVYDTV
                     GGAPSHVALVVGDGTVIHAVSAGTKTGVIVSPMSDRYWAPRLLGARSFIATSTATVSA
                     APIAPASP"
     gene            716..>1018
                     /locus_tag="PLQ29_14955"
     CDS             716..>1018
                     /locus_tag="PLQ29_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87993.1"
                     /translation="MKRFVCLAMIATTLSIGAFGQSYAALPTGSVGSADPQFALSILT
                     DYFEKHERSSRTGGVVLSSIGGALLAGGLAGVGYSFAAPSTSFSDPEGQMLMRTNSR"
BASE COUNT          188 a          330 c          331 g          169 t
ORIGIN      
        1 gggcgaggcg ggtgctatcg gcgctgccga cacggtcgcc gtcgacgtag cgatgaacga
       61 gcgggcgccg agcaggcgtg gagcccagta gcggtcgctc atcggcgaga cgatgacgcc
      121 ggtcttcgtc ccggccgata cggcgtggat gacggtgccg tcgccgacga ccagcgcgac
      181 gtggctcggg gcaccgccga ccgtgtcata gaccagcacg tcgccggccc gggccttgcg
      241 ccagtctatg ggcgagccgc tcgaccagaa cgaccgcgcg ctacgcggca gctcgaggcc
      301 gagcgcttcg cgatacacgt agcggacaaa gcccgagcag tcgaaggctc ggggggactc
      361 ggcgccgtag acgtacggta ccccccggta cttgaaagcg gcgtcgacga gcctgcgcct
      421 ggcctcatcc tcagaactct gagcgaaggc cgaacccgcg agcagcgcca gggccacgat
      481 acacccgatt cgtttcatat ccataccacg actatccgtc ggcctcgccc gctcgtcaag
      541 cgcgaggcgt ctccatgtgc tatgctatcg aaggtgatag cgatgaacta tcgattctga
      601 tatgagtgag gagcggcaag cttgtagaac ggccttcgag ccataccata accatggcac
      661 gcttattgca gtattaccgc taggcgcccg agtatcgagc gccgggaggt tgaccatgaa
      721 acgattcgtc tgtctagcga tgattgccac gacgctgtcg atcggagcct tcggccagtc
      781 ctacgccgcg ctaccgaccg gttcggtcgg ctcggccgac ccgcagttcg ccttatccat
      841 actgaccgac tacttcgaga aacacgagcg ctcgagcagg accggcggcg tcgtgctgtc
      901 gagcataggc ggcgccctgc tcgccggggg cctggcgggc gtcggctatt cgttcgccgc
      961 accgtcgacg agcttctccg atcccgaggg tcagatgctg atgcgaacga atagccga
//
LOCUS       DAOPHV010000858         1018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_226469_length_1018_cov_3.544133, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000858 DAOPHV010000000
VERSION     DAOPHV010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_226469_length_1018_cov_3.544133"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..287)
                     /locus_tag="PLQ29_14960"
                     /pseudo
     CDS             complement(1..287)
                     /locus_tag="PLQ29_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519007.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(294..>1018)
                     /locus_tag="PLQ29_14965"
     CDS             complement(294..>1018)
                     /locus_tag="PLQ29_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87994.1"
                     /translation="TGTNGTLFAIAANGRSTSDAAAYSIYYLDGGSFVASSIADSAAI
                     GLPSSVAYVSGSYWFAAGAALVSGASAGALSVAATEPPSGDSSYSGVCAYDSGVIVST
                     RTGYLHYYDGAAWTSAGPFVNAKDKVFSLSEPTYVASDKVLLVGTNMKPRGSSDTPPV
                     DGYLEFDLSGGFSAAIAPLAAHSIVSDATNFAASLSGTSITSMPSFDLGGGKRRIFAL
                     TDGVGPWSNTYENGAWGGWVRE"
BASE COUNT          180 a          316 c          364 g          158 t
ORIGIN      
        1 cggcgtcgcg tatggcctgg acgccggcgc accgcgttaa tcgaaatgaa acgcgagctg
       61 gtcgtgttgg ctatgactcg ggcgagcgtc gtcttgccgg tgcccggagg accggagagg
      121 atgatcgacg acaagcggtc gcgctggatc gcccgcctga gcagacggcc ggggccgacg
      181 atgtggtcct ggcctatgaa ctcgtcgagg acgcgcggcc gcatcctgtc ggccagcggg
      241 ccgcacccgt ccgtatggac ggagcccgag aataagtcct cgctcacggc ccgctattcc
      301 ctgacccagc ctccccaggc gccgttctcg taggtgttgg accatgggcc gacaccgtcg
      361 gtcagagcga atatcctgcg cttgccaccg ccaaggtcga acgagggcat cgaggtgatc
      421 gacgtgcctg acaggctcgc cgcgaaattc gtggcgtccg atacgatcga atgggcggct
      481 agaggggcga tagccgccga gaaaccgccc gacaggtcga actccaggta gccgtcgacc
      541 gggggagtgt cgctcgagcc gcgcggcttc atgttcgtgc cgacgagcag gaccttgtcc
      601 gaggcgacgt aggtaggctc ggacagcgag aagaccttgt ccttagcgtt gacgaatgga
      661 ccggcgctcg tccaggcggc gccgtcgtag tagtgcaggt agccggtcct cgtcgagacg
      721 atgacgccgc tatcgtaggc gcagacgccg gaatacgatg agtccccgga aggcggctcg
      781 gtcgctgcta cggacagcgc gccggcggac gcgccggaca cgagggccgc gccggcggcg
      841 aaccaatagg agccgctgac gtaagcgacc gaggacggca ggcctatcgc cgcgctatcg
      901 gcgatggagc tggctacgaa ggagccgcca tctaggtagt agatcgagta ggcggcggca
      961 tcgctcgtgc tcctgccgtt cgcagcgatc gcgaacaggg tgccgttcgt gccggtgc
//
LOCUS       DAOPHV010000859         1017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_226892_length_1017_cov_2.641372, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000859 DAOPHV010000000
VERSION     DAOPHV010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_226892_length_1017_cov_2.641372"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PLQ29_14970"
     CDS             <1..808
                     /locus_tag="PLQ29_14970"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HPG87995.1"
                     /translation="HFKELFPEYNARMPVVPQILSNDAAGFVATASALEALGYVEVNW
                     NLGCPYPMVTRKRRGAGLLPHPDVVRAFLEEACGRIRLELSIKLRLGLADSAEILGLM
                     PILNDYPLRSVILHPRVGAQLYEGSVDLDAFAAAASLSTRELAYNGDIVDEASYATIR
                     ARFPSVSEFMLGRGVVSDPFLPARIKGLGLPDDPKATIKAFHDDVYFSYRETLSGRTH
                     ALDKMKELWSYLGASFARSEDGLKEIRKAASFEAYEKAVRMAFSDDTAQQ"
     gene            complement(851..>1017)
                     /locus_tag="PLQ29_14975"
     CDS             complement(851..>1017)
                     /locus_tag="PLQ29_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPG87996.1"
                     /translation="DRDSGQSKGFGFVEMGSDAEAQAAIQGINGREVDGRQLKVNEAM
                     DKPRRDNNRY"
BASE COUNT          174 a          318 c          335 g          190 t
ORIGIN      
        1 ccacttcaag gagctattcc cggagtacaa cgcgcgcatg ccggtcgtgc cgcagatcct
       61 gagcaacgat gcggcgggct tcgtcgcgac ggcctcggcg ctcgaggcgt taggctacgt
      121 cgaggtgaac tggaacctag gctgcccgta cccgatggtc acgcgtaagc ggcgcggggc
      181 ggggctattg ccgcatcccg acgtagtcag ggccttcctc gaggaggctt gcggtcgtat
      241 acggctcgag ctgtcgatca agctacggct cggcctcgcg gactcggcgg agattctcgg
      301 gctcatgccg atcctgaacg attatccgct acgctcggtc atactgcacc cacgggtcgg
      361 cgcgcagctg tacgaaggct cggtagacct ggacgccttc gccgcggccg cgagcctgtc
      421 gacgcgcgag ctcgcgtata acggggatat cgtcgacgag gcctcgtacg cgacgatacg
      481 cgcccgcttc ccttccgtga gcgagttcat gctggggcgg ggcgtcgtat ccgatccctt
      541 cctgccggcc aggatcaagg gcctaggcct gcccgacgat cccaaggcga cgatcaaggc
      601 gttccatgac gatgtgtatt tcagctacag ggaaacgctg tcgggacgga cgcacgcgct
      661 cgacaagatg aaggagctat ggtcgtacct gggcgcctcg ttcgcgcgct cggaggacgg
      721 gcttaaggag atccgtaagg cggcgagttt cgaagcctac gagaaggcgg ttcggatggc
      781 gttttccgac gacacggcgc agcaataaaa aaaccggcct cgcggccggt tccttatcca
      841 cggttactga ttaatagcgg ttgttgtcgc ggcgcggctt atccatcgcc tcgttcacct
      901 tgagctgcct gccgtcgacc tcgcggccgt tgatgccctg gatggcggcc tgcgcctcgg
      961 catccgagcc catctcgacg aagccgaagc ccttggactg gccggagtcg cgatcgg
//
LOCUS       DAOPHV010000860         1013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_228324_length_1013_cov_2.964509, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000860 DAOPHV010000000
VERSION     DAOPHV010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228324_length_1013_cov_2.964509"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PLQ29_14980"
     CDS             complement(<1..547)
                     /locus_tag="PLQ29_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPG87997.1"
                     /translation="MSAFDSVRLELVELTKAWPGRTVSVSMTAEAGGILAVAGPSGCG
                     KSTALRMAAGLECPDSGRVIVGGRDVSSLEPSQRGIGMVFQDYALFPHLDVAGNIEYG
                     LKTRGIGAAERRATVARLLDSLGLAGFERRRPRELSGGERQRVALARTLAVEPAVVLF
                     DEPLSSLDAALRKRLRLDIVEE"
     gene            complement(534..>1013)
                     /locus_tag="PLQ29_14985"
     CDS             complement(534..>1013)
                     /locus_tag="PLQ29_14985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPG87998.1"
                     /translation="PFLSKTLPLLPLGVSGVVLAYGWNSALGRSSTLAVAAVQAVSAY
                     PFALRAIQSSVGLSDERYVEAARCLGSSRLGAALRVRLPMAASSIASGFAFAFAMSAG
                     DANALIAAPVAGFETLALMLYRLAGSYRLNEACAAAVLLAIGTGFIFFIKDASDVRL"
BASE COUNT          187 a          348 c          351 g          127 t
ORIGIN      
        1 gctcctccac gatgtcgagg cgcaggcgct tacgtagcgc ggcgtcgaga gacgaaagag
       61 gctcgtcgaa cagcacgacc gccggttcga cagccagggt cctggctagg gccacccgct
      121 ggcgctcgcc gccggacagc tcccgtgggc gcctacgctc gaagcctgcc aggccgagcg
      181 aatcgagcag tctggccacg gtggcgcggc gttcagcggc cccgatacca cgggtcttga
      241 gtccgtactc gatattgcca gccacgtcca ggtgcgggaa cagcgcgtag tcctggaaga
      301 ccatgccgat gcctcgctgg ctcggctcga gggagctcac gtcgcgccct ccgacgataa
      361 cccggcccga gtccgggcac tcgagccccg cggccatcct gagcgccgtg ctcttgccgc
      421 aaccggaggg cccggccacg gccaggatgc cgccggcctc agcggtcatg gatacgctga
      481 ccgtccggcc gggccaagcc ttggtcagct cgaccagctc caggcgtact gaatcaaagg
      541 cggacatcgc tcgcgtcctt gatgaagaag atgaagccag ttccgatggc cagcagcacc
      601 gccgcggcgc aggcctcgtt cagcctatag gaaccggcca atcggtagag catcagggcc
      661 agcgtctcga agccggcgac cggggccgct atcaacgcgt tcgcgtcgcc ggcgctcatc
      721 gcgaacgcga acgcgaaacc agaggctatc gaggaggcgg ccatcggtag ccgtacgcgc
      781 agcgcggcgc cgagccgcga cgagccgagg cagcgggccg cctcgacgta gcgctcgtcg
      841 gaaaggccca ccgacgactg gatggcgcgt agcgcgaagg ggtaggccga gacggcctgc
      901 accgccgcga cggccaaggt ggaactccgc ccgagcgccg agttccaacc gtaggcgagc
      961 acgacgcccg atacgccgag cggcaagagg ggtagggtct tggacaagaa ggg
//
LOCUS       DAOPHV010000861         1012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_228706_length_1012_cov_2.815047, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000861 DAOPHV010000000
VERSION     DAOPHV010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228706_length_1012_cov_2.815047"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1012)
                     /locus_tag="PLQ29_14990"
     CDS             complement(<1..>1012)
                     /locus_tag="PLQ29_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPG87999.1"
                     /translation="KASVSLLVSTVLAAGFAALAYSGLFSIIETRFFDERVRRSVDAS
                     VDGLLESSDAYHESNAARFEALLGNDFIKRSFLTNQSAQDSFDRTRAFGLLAEQTPGL
                     VGVRFIDRDGKRIHFSTFPADAVRSEALRVVYRDYGEPGDAPYAALEPPEGSGSGSGS
                     IAVSSDPARRAFAYRFPFVDGFEVRRGTAVFYVSYSGLLERLVKDGRIALGDDAVAIG
                     DGGILFGSPSWGGNELVSRVAEIWAAGPNAEPIPVGSAEASDSFLLFSRRGASGLVGR
                     LVPASWFAMPEAFRWLLLTAFFVTVYLILFLVLNLRQDRFAVLAARIKRFQIELLEEY
                     LER"
BASE COUNT          190 a          320 c          359 g          143 t
ORIGIN      
        1 tgcgttcgag gtactcctcg agcagctcga tctggaagcg cttgatcctg gccgccagca
       61 cggcaaaacg gtcctgcctc aggttgagga cgaggaaaag gatcaggtag acggtaacga
      121 agaaggcggt caggaggagc caacggaagg cttccggcat ggcgaaccac gaggccggta
      181 cgagcctgcc gaccagcccg gacgcgccgc gcctagagaa cagcaggaac gagtccgaag
      241 cctcggctga accgacgggg ataggctcgg cgttcgggcc ggcggcccag atctcggcta
      301 cgcgcgagac gagctcgttg ccgccccacg acggcgagcc gaacagtatg ccgccgtcgc
      361 cgatggccac ggcgtcgtcg ccgagcgcta tgcgcccgtc cttgacgagc ctctcgagca
      421 ggccggagta cgacacgtag aatacggcgg tgccgcggcg cacttcgaag ccgtcgacga
      481 acgggaaacg gtacgcgaac gcgcgcctgg ccgggtcgga cgatacggct atcgagccgg
      541 agccgctccc cgagccctcg ggcggctcca gcgcggcgta cggggcgtcg cccggttcac
      601 cgtagtcgcg gtagactact cggagcgcct cggaacgcac agcgtcggcg gggaaggtgc
      661 tgaaatggat gcgctttccg tccctgtcta tgaagcggac gccgacgagg ccgggcgtct
      721 gctcggcgag caggccgaag gccctggtac ggtcgaacga gtcctgggcg ctctggttgg
      781 tcaggaagct acgcttgatg aagtcgttgc cgagcagggc ctcgaagcgg gccgcgttcg
      841 actcgtggta cgcgtccgac gactcgagca gcccgtcgac cgaggcgtcg accgatcgcc
      901 tgacccgctc gtcgaagaac ctggtctcta tgatcgagaa cagccccgag tacgcgagcg
      961 cggcgaagcc ggccgccagc acggttgaga cgagtaggct tactgaggct tt
//
LOCUS       DAOPHV010000862         1006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_230935_length_1006_cov_2.583596, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000862 DAOPHV010000000
VERSION     DAOPHV010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_230935_length_1006_cov_2.583596"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLQ29_14995"
     CDS             <1..364
                     /locus_tag="PLQ29_14995"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPG88000.1"
                     /translation="KALGVSLSVDDFGTGYSSLSYLKNLPIDMVKIDRDFIKDIETSN
                     AAREIVSGIVALAHRMNLSVVAEGVEAAEQFYLLKSMGCDFAQGFLFSHALPPLELEG
                     RYLSRGTGSRYSSFLLRR"
     gene            complement(375..>1006)
                     /locus_tag="PLQ29_15000"
     CDS             complement(375..>1006)
                     /locus_tag="PLQ29_15000"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPG88001.1"
                     /translation="GRGVGADDFMMLAFGVIVALYAADTVKKSVLPSEERLRALSLEN
                     EELWNLSFRDTLTGLYNRRYIQQIAIHLFSRAVRYKEQLHVLMIDIDHFKRVNDKLGH
                     AVGDEVLKGVAQTIQATIRTSDTVARFGGEEFIVYMVRSDPETTQFIANRIRDGVAAV
                     QFPGVPWQITISVGVAGLQENDTIDSLVERSDQFLYVSKHRGRNRVSGF"
BASE COUNT          186 a          318 c          306 g          196 t
ORIGIN      
        1 caaggcgctc ggcgtgtcgc tgtcggtcga cgacttcggc accggctact cgtcgctgtc
       61 gtacctgaag aacctgccca tcgacatggt gaagatagac cgcgacttca tcaaggacat
      121 cgagacgtcg aacgccgcga gggagatagt cagcggcatc gtcgcgttgg cccaccgcat
      181 gaacctttcg gtggtcgccg aaggtgtgga ggcggcggag cagttctacc tactcaagtc
      241 catgggctgc gacttcgccc agggcttcct gttcagccac gcgctaccgc cgctcgagct
      301 cgagggcagg tacctgtccc gcggaaccgg ctcgcgctac tcgtcgttcc tgctacgccg
      361 ctaagcgccg cgggctaaaa gccggagacg cggttacggc cgcgatgctt cgacacgtag
      421 aggaactgat ccgaccgttc caccagcgag tctatggtgt cgttctcctg gaggccggcc
      481 acgcctacgc tgatggtgat ctgccagggc acaccgggga actggaccgc ggctacgccg
      541 tcgcggatcc tgttggctat gaactgcgta gtctcaggat ccgacctgac catgtacacg
      601 atgaattcct cgccgccgaa gcgggctacc gtgtcgctcg tgcgtatggt ggcctggatg
      661 gtctgagcca cgcccttgag cacctcgtca ccgacggcgt gcccgagctt gtcgttgacc
      721 cgcttaaaat ggtcaatgtc tatcataagc acgtgtagct gctccttgta gcgtacggcc
      781 cgcgaaaaca ggtggatggc gatctgctgg atatagcgac gattatagag gccggtcagc
      841 gtatccctga acgaaaggtt ccatagctcc tcgttctcga gactcagggc gcgcagtcgc
      901 tcctcgctcg gcaacacgct cttcttcacg gtgtcggcgg cgtacagggc cacgatcacg
      961 ccgaacgcga gcatcatgaa gtcgtcggca ccgacgcctc gcccaa
//
LOCUS       DAOPHV010000863         1006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_230946_length_1006_cov_2.528917, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000863 DAOPHV010000000
VERSION     DAOPHV010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_230946_length_1006_cov_2.528917"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PLQ29_15005"
     CDS             <1..614
                     /locus_tag="PLQ29_15005"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPG88002.1"
                     /translation="VKLLQVLQQKEVTPLGSNRPVKMNARVVAATNADLAALVESGAF
                     RRDLYFRLKVVEIYVPPLRERRDDIPLLINHFIASFAGEMGKDVRALSGDAARELLEY
                     DYPGNVRELANIVEHGVLMADGPSIRLADLPPELAGSKPACPEPAEADLAAYLGGKSL
                     AELEAIAIKAALERNGGRRRETATSLGISERGLRNKIHEYGLG"
BASE COUNT          208 a          336 c          308 g          154 t
ORIGIN      
        1 aggtgaagct cctccaggtc ctccagcaga aggaggtgac gccgctcggt tccaaccgcc
       61 cggtcaagat gaacgcccgc gtggtcgcgg cgaccaatgc cgacctggcg gccctcgtcg
      121 agtcgggcgc tttccggcgc gacctgtact ttaggctcaa ggtcgtcgag atctacgtgc
      181 cgccgctccg cgagcggcgc gacgacatac cgctcctgat caaccatttc atcgcctcgt
      241 tcgccggcga gatgggaaag gacgtccggg cgctctccgg cgatgcggcc agggagctgc
      301 tcgagtacga ctacccgggc aacgtcaggg agctggccaa catcgtcgag cacggcgtgc
      361 tgatggccga cggcccctcg atacggctgg cggacctgcc ccctgagctg gcaggatcca
      421 agcctgcctg ccccgagcca gccgaggcgg atctagcggc ctatctcggc ggcaagagcc
      481 tcgcggagct cgaggccata gcgatcaagg ccgcactcga acgaaacggc ggccggcgtc
      541 gcgagacggc cacgagccta ggcatcagcg agcgcgggct acgcaacaag atccacgagt
      601 acggcctcgg ctgagtcgtc agagcggcag caaatgccga gagcggcaaa tactaccgtt
      661 cgatatgaga cagggagtca taaccagctc ccaccagccg tcgccctaaa gagtgccacg
      721 ctataccaga catgcaatta gataggctta tgcgcgacag tccgagacgc ggcgtaccat
      781 ggcacgaatc ttgcaatacg tttaggtagc actacgttcc caaggaggtt cccatgaagc
      841 actcgaggat catcaccgta atcgccatcg tagcgatcgc cgccgtggcg ttctcgtcct
      901 gcgccaagaa ggaagaggcg gccaagcccg ccgcgatcga caagaactcc atcttcatca
      961 ccgtcgcgtc cggccccacc agcggcctgt actacccgat cggcgg
//
LOCUS       DAOPHV010000864         1005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_231270_length_1005_cov_3.047368, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000864 DAOPHV010000000
VERSION     DAOPHV010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_231270_length_1005_cov_3.047368"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..>1005
                     /locus_tag="PLQ29_15010"
     CDS             159..>1005
                     /locus_tag="PLQ29_15010"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPG88003.1"
                     /translation="MRRIACIVAVAAVASAGVAAQGAGDTVDLGQAVVVGAWAPSELS
                     PTAATVVIDAEEIAASGASTLDELLDGRAGLELREYGYPGSAGSLSVRGGAGGHVVVV
                     VDGVRLNDARTGYADLSGVSLAGVESVEVLRGGASALYGADAIAGVVYVTTARARSDS
                     FSATVSTTAYPLAAAEGASALLASQELSFAARKAVGAATVSVSGGAERAADELPVADE
                     ESYSVRGNTGLLAGRAGLGVVSPLLGGTVTASASGSVSDKGVPGSVSYASPNAYQKEY
                     SARGII"
BASE COUNT          137 a          344 c          381 g          143 t
ORIGIN      
        1 cggggccgcc ttcgaggatc gaggagtccc tgcatgaaac ccgccttccc ggcgggccgg
       61 cgtcgccgct gcgactgacc ggtccgcgac gataccgcca atttacaggc tcatcgtcct
      121 ctctcgtccg gcgtgaccgg acggaaagga ttcgcatcat gcgcaggatc gcatgcatcg
      181 tagcggtcgc cgccgtagcc tcggccggcg tcgcggccca gggggcgggc gataccgtcg
      241 acctgggcca ggccgtcgtc gtcggcgcct gggccccgtc ggagctgtcg ccgaccgcgg
      301 cgacggtggt catcgacgcc gaggagatag ccgcctcggg cgcgagcacg ctggacgagc
      361 tgctcgacgg ccgcgccggg ctcgagctgc gggagtacgg gtatcccggc tcggccggct
      421 cgctgtcggt acgcggcggc gctggcggcc acgtcgtcgt cgtggtggac ggcgtccggc
      481 tgaacgacgc ccgcaccggt tacgccgacc tctcgggcgt atcgctggcc ggcgtcgaga
      541 gcgtcgaggt gttgcgcggc ggcgcgtcgg cgctgtacgg ggcggacgcg atcgccggcg
      601 tggtctacgt gacgacggcg agggccagaa gcgattcgtt ctcggcgacc gtctcgacga
      661 cggcgtatcc tctcgcggcg gcggagggcg cgagcgcgct gctggcgagc caggagctga
      721 gcttcgccgc gcggaaagcc gtcggggccg cgaccgtcag cgtctcgggc ggcgcggaaa
      781 gggccgccga cgagctaccg gtggccgacg aagaaagcta ctcagtacgc ggaaataccg
      841 gtctcctggc cggacgcgcc ggcctcggcg tggtctcgcc gctcctcggc ggaacggtaa
      901 cggcgtccgc gagcggctcc gtctccgaca aaggcgtgcc cggcagcgtc tcgtacgcgt
      961 ccccgaacgc ctaccagaag gagtactcgg ccaggggtat catag
//
LOCUS       DAOPHV010000865         1005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_231330_length_1005_cov_2.580000, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000865 DAOPHV010000000
VERSION     DAOPHV010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_231330_length_1005_cov_2.580000"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PLQ29_15015"
     CDS             <1..686
                     /locus_tag="PLQ29_15015"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HPG88004.1"
                     /translation="EWLDAGLDELRFSPPRFERPTDGAPRLRVLHEVETRTGRPAGRF
                     VQSLRFGRDEVLVECIFDLDPGLPELPRVGLGTALAPLFEKARWYGRGPHECYPDRKA
                     GAALGVWESSVDGLQVPYVLPQENGNRCDVRWVELASDETALRIDGWPAFNFVASHHE
                     TEQLWKATHTHELARRPETYLRLDAAQRGLGTASCGPDTLERYRVYPAAYRLSLRLAP
                     RPARGRAAP"
     gene            complement(688..>1005)
                     /locus_tag="PLQ29_15020"
     CDS             complement(688..>1005)
                     /locus_tag="PLQ29_15020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPG88005.1"
                     /translation="RASPDRRRILFGGRVSLSCRSRADGVEVRVADTGIGMDEETARR
                     MFDKFYQGDRSRASRGNGLGLSIAKRIVDLYGGRVSVRSAPGRGTTITVVIPRLGQAA
                     IEG"
BASE COUNT          149 a          389 c          330 g          137 t
ORIGIN      
        1 acgaatggct cgacgccggc ctggacgagc tacgcttctc cccaccgcgc ttcgagcgcc
       61 cgaccgacgg cgcgccccgc ctgcgcgtgc tccacgaagt cgagacgcgg accggcaggc
      121 ctgccggccg cttcgtccag tccctacgct tcggcagaga cgaggtcctg gtcgagtgca
      181 tcttcgacct ggaccccggc ctgcctgaac tgccccgggt cgggctcggt accgcgttgg
      241 cgccgctctt cgagaaggcg cgctggtacg gccgaggacc ccacgaatgc taccccgacc
      301 gcaaggccgg ggcggccctc ggcgtctggg aatcgagcgt cgatgggctc caggtgccct
      361 acgtgctacc gcaggagaac ggcaaccgct gcgacgtccg ctgggtcgag ctcgcgagcg
      421 acgaaaccgc cctgcgcatc gacggttggc cggcgttcaa cttcgtcgcc tcccaccacg
      481 agacggaaca gctttggaaa gccacccata cccacgagct cgcgcgccgt ccggagacct
      541 acctgcgtct cgacgcggcc cagcgcggcc tcggcacggc cagctgcgga ccggacacgc
      601 tcgaacgcta cagggtctac ccggctgcct atcgcctatc gctacgactg gcgccgcgcc
      661 ccgcacgagg ccgagccgcg ccctgagcta gccctctatc gccgcctgcc cgagcctcgg
      721 tatgacgacc gtgatcgtcg tgccgcggcc cggggcgcta cgcaccgaga cgcggccgcc
      781 gtacaggtcg acgatgcgct tggctatcga caggccgagg ccgttgcccc tcgaggcgcg
      841 cgaccggtcg ccctggtaga acttgtcgaa catgcgccgg gcggtctcct cgtccatgcc
      901 gatgccggta tccgccacgc gcacctcgac gccgtcggcg cggctccggc acgacagcga
      961 cacccggccg ccgaacagga tgcggcgccg gtccggcgag gcgcg
//
LOCUS       DAOPHV010000866         1004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_231594_length_1004_cov_3.849315, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000866 DAOPHV010000000
VERSION     DAOPHV010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_231594_length_1004_cov_3.849315"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PLQ29_15025"
     CDS             complement(<1..583)
                     /locus_tag="PLQ29_15025"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPG88006.1"
                     /translation="MRLACVGNALYDVVAFVETDFASRLGFHAGSTVHLDRDGMEPIL
                     SALAKSSRGAGGGAVNTAKVFASLGHGAAFAGMVGDDELGTLFRRDLEACGIASYLQV
                     GRGRTGAFCAMIAPDGERTIAVAPELAPRLDPSAIPDEFFASDSTMYVDGFIAANPDA
                     LRALTRRAADSGMGIALDVAGYRIAVKHRDIEGY"
     gene            712..785
                     /locus_tag="PLQ29_15030"
     tRNA            712..785
                     /locus_tag="PLQ29_15030"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:746..748,aa:Val,seq:tac)
BASE COUNT          184 a          318 c          341 g          161 t
ORIGIN      
        1 tatagccctc gatgtcgcga tgcttgacgg cgatgcggta ccccgcgaca tcgagggcta
       61 tacccatgcc tgagtcggcg gcgcggcgcg tcagggcccg aagcgcgtcc gggttcgcgg
      121 cgatgaagcc gtcgacgtac atcgttgagt cggaggcgaa gaactcatca gggatggccg
      181 acgggtcgag gcgaggggcg agttccggcg cgaccgctat cgtgcgctcg ccgtccgggg
      241 ctatcatcgc gcagaaggcg ccggtacggc ctcgtcctac ctggaggtag gacgcaatgc
      301 cgcaggcctc gaggtcgcgg cggaatagtg tacccagctc gtcgtcgccc accatgcccg
      361 cgaacgcggc gccgtgcccg agcgaggcga ataccttggc cgtattgacg gcgcctccgc
      421 cagcgcctcg cgatgactta gctaaggccg ataggatggg ctccatgccg tcacggtcga
      481 ggtggaccgt cgaaccggcg tggaaaccga gacgggacgc gaagtccgtc tccacgaacg
      541 ccacgacgtc gtataacgcg ttcccgacgc aggcaagcct cacgggtacc tctccgattc
      601 catgagccat tctaccatga tccgtcgaac aaagccatgc cggaatcgag gaggcgtccg
      661 gcgcccgtct tcttgacccc cgctcgtccc gcgtggtatc gtctcgcgtc ggggcgctta
      721 gctcagctgg tcagagcgcc tggtttacac ccaggatgtc gggggttcaa atccctcagc
      781 gcccaagaaa agcccgttcg gcctttggcc gggcgggctt ttatgataag ggcgccgagg
      841 gatttgaacc gagcgttgtg gcgagcgaaa gcgagcccga ggcgcaggaa gcgccgagaa
      901 acgcgaaggc gggctgacgt gagcgcacag gacgcgcgcg agcggaagcc aaatccctca
      961 gcgcccaaga aaagcccgtt cggcctttgg ccgggcgggc tttt
//
LOCUS       DAOPHV010000867         1001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetales bacterium isolate AMR_MDS_3320
            NODE_232807_length_1001_cov_3.181818, whole genome shotgun
            sequence.
ACCESSION   DAOPHV010000867 DAOPHV010000000
VERSION     DAOPHV010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865055
            Sequence Read Archive: SRR5007219
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetales bacterium (wastewater metagenome)
  ORGANISM  Spirochaetales bacterium
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 20:26:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,978
            Genes (coding)                    :: 2,955
            CDSs (with protein)               :: 2,955
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 7 of 23
            Pseudo Genes (incomplete)         :: 11 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Spirochaetales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_232807_length_1001_cov_3.181818"
                     /isolate="AMR_MDS_3320"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026792"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.12 N 87.65 W"
                     /collection_date="2015-03-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1001)
                     /locus_tag="PLQ29_15035"
     CDS             complement(<1..>1001)
                     /locus_tag="PLQ29_15035"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HPG88007.1"
                     /translation="ATGAAATGAAATGAAATGAAATGGNHAAGTVFYIDWPGWAGRGK
                     GEGGGSAAMLSMSADKERYDVGQTVSVSFPSNKEGRAFVTIERAGRVLREEWVEGKDG
                     TTVYSFKATADMAPNVYVHASFVQPHLQTLNDLPIRLYGVVPVMVEDPATRLQPVIAA
                     PASLEPMKKAVVTVSERSGKPMTYTLAAVEEGLLGITRYAAPNPWSEFYKKEASLLRS
                     FDLYKDVAGAYSGKLQTLIAIGGSEFGDSGGTRKVSRFPPVVKYFGPFLLERGAKASH
                     ELELGPYVGSVRLMVVAGTPDGAYGKAELETPVRSELMAYLTAPRVLGPGETVTLPVT
                     "
BASE COUNT          148 a          330 c          353 g          170 t
ORIGIN      
        1 gtcaccggca gcgtcaccgt ctcgccgggg ccgagcacgc gcggcgcggt caggtaggcc
       61 atcagctcgg agcgaaccgg cgtctcgagc tcggccttgc cataggcgcc gtcgggcgtg
      121 ccggcgacga ccatcaggcg caccgagccg acgtacggcc cgagctcgag ctcgtggctg
      181 gccttggctc cgcgctcgag caggaacggc ccgaagtact tgacgaccgg cgggaagcga
      241 ctgaccttgc gggtgccgcc tgagtccccg aattcggagc cgcctatagc tatgagggtc
      301 tggagcttgc ccgagtaggc gccggccacg tccttataca ggtcgaagga gcgtagcaga
      361 ctagcttcct tcttgtagaa ctcgctccac gggttcggcg cggcatagcg ggtgatgccg
      421 aggagacctt cttcgaccgc ggccagggta taggtcattg gcttgccgga tcgctcggat
      481 accgtaacca cggccttctt catcggctcg aggctggcgg gggccgcgat gaccggctgg
      541 agcctggtgg ccggatcctc gaccatcacc ggcacgacgc cgtagaggcg tatcggtagg
      601 tcattcaggg tctgcaggtg gggctggacg aagctggcgt gcacgtagac gttaggcgcc
      661 atgtcggcgg tggccttgaa gctatagacg gtggtgccgt ccttgccctc gacccattcc
      721 tcgcgcagca cgcggccggc tcgctcgatc gtcacgaagg cgcggccttc cttattcgac
      781 gggaagctga ccgacacggt ctgcccgacg tcgtagcgct ccttgtcggc gctcatcgaa
      841 agcatcgcgg ccgagccgcc gccctcgccc ttgccgcggc cggcccagcc cggccagtcg
      901 atatagaaga ccgtgccggc ggcatggttg ccgcctgtgg cggcagcgcc tgtggcggca
      961 gcgcctgtgg cggcagcgcc tgtggcggca gcgcctgtgg c
//
